LOCUS       DAOQTZ010000001        87959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_314_length_87959_cov_13.329018, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000001 DAOQTZ010000000
VERSION     DAOQTZ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 87959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 87959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87959
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_314_length_87959_cov_13.329018"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PKN51_00005"
     CDS             complement(<1..1058)
                     /locus_tag="PKN51_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83505.1"
                     /translation="MSARAVDAVSALLAAALLAALLGLSVWLWQGGQRALLLAPAIGE
                     LADCLELAPAQTPLEPACSGERGSAAQRIEATLGPLGPRRSADGHFELGYTLVVPLLN
                     LFEPQGAGWAIDQQAVQRIVRTVRDVQRPVVLYLFSTHFSEQAPIEPVLAQDPANLAH
                     TPQGPLPVDQFMGWPLYPWSIARTDNAITQRREQAIGALVQGVCALPAAARQRIVGLN
                     LLGEVHHLYPDFEAGMGHDRPYVLTDYADASRRGFRAFLRQRFGHVAALNAYLGSDFA
                     SFDAVDPPSRDIRREPLQHFWQHLDDAAAGTLAISGWAHDAALPAGATPWVRVYLDGL
                     PVARVPAHFVRQDVGQALP"
     gene            complement(1239..1778)
                     /gene="msrA"
                     /locus_tag="PKN51_00010"
     CDS             complement(1239..1778)
                     /gene="msrA"
                     /locus_tag="PKN51_00010"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNT83506.1"
                     /translation="MSDSQTLVLAGGCFWCTEAVFKQVRGVLDVESGYSNGQATAPSY
                     EQVCTGATGCAEVVKLVYDPGQIGTRQILEIFFLVHDPTQLNRQGHDVGTQYRSGVYF
                     TTAEQADVAHALIREMSQDGLYDGAIVTEVAPLAHYFPAEDYHQDFFERNPTQGYCVA
                     TAAPKVAKFRQTFRELVKA"
     gene            2101..3147
                     /locus_tag="PKN51_00015"
     CDS             2101..3147
                     /locus_tag="PKN51_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HNT83507.1"
                     /translation="MPHPPTPRRWRAARLVPALCAGLLAGLAPPAQAAWEPTAPVELV
                     VPAGTGGGADQMARFIQQMVSKHGLMKQPLNVVNKTGNSGVEGLMDVKAAQGNPHKLI
                     ITLSNLFTAPLATGTPFAWSDITPVQMLALDEFVLWVAKGSPYKSPKDVLDALRASPA
                     GSLRLGGTGTKQEDQLISVLLETAASARIQYVPFKGGGDVARALAAGEVDLTVNNPIE
                     AEKLWREGKLRPLCVFDGSKLAYPDKVADNQSWSDLPTCMSFGIPVEYLMMRGIFMAP
                     GATPEQVAYYAQLLDKVRALPEWNALMKQGAYKQTTMAGPSFSSWLDRSNRFHRTLMR
                     EARLTAAAPGAARN"
     gene            complement(3159..3641)
                     /locus_tag="PKN51_00020"
     CDS             complement(3159..3641)
                     /locus_tag="PKN51_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNT83508.1"
                     /translation="MTTPRQYWLMKSEPDECSIDDALAAPGQTVPWTGVRNHQARNFM
                     RDAMRVGDGVLFYHSSCAEPGIAGIARVASAAHADPTQFDPGSAYFDPTARPDSPRWL
                     LVDVQALRKTRLLGLAELRGVPALADMLVLRRGNRLSITPVTPGQWQAIERLLAAAPA
                     "
     gene            3673..4722
                     /locus_tag="PKN51_00025"
     CDS             3673..4722
                     /locus_tag="PKN51_00025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNT83509.1"
                     /translation="MTDHAHPFATLTPDFVLDALAALGLMGDGRLMTLNSYENRVYLA
                     HLEPGTPLAEAHPAVVLKFYRPGRWTQAQIEEEHAFARELAAAEVPVVAPLVIDGRTL
                     HRHGDFWFSLTPRRGGRAPELGDADTLAWMGRFLARLHLVGARQAFVHRPALDLRSHG
                     VEPREWLLAHAALPPEAERAWAEVSQDAIDHIAAGADWASSETHFDHPTRPIATLRLH
                     ADCHVGNILWTPTEQPGGGPHFVDLDDCRSGPAVQDLWMLLAGERAERQRQLGALLDG
                     YEQVRAFDRRELALIEPLRTLRLIHYSAWLAQRAHDPAFAAAFAGFGTPDYWRGQTAL
                     LREQIEAMAEPPLCA"
     gene            4829..5488
                     /locus_tag="PKN51_00030"
     CDS             4829..5488
                     /locus_tag="PKN51_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT83510.1"
                     /translation="MNTLQDLPCPPSRRTRRKQARPGELLDAALALFVEKGFAATRVE
                     EVATRAGVSKGTLFLYFPSKQALFKAVVRENVGRHLDQALADLASYTGSSADLLREFL
                     RRWWTHYGNTPAAGLCKLIVVEVAHFPELARDYQEQVVLPSHELLRRVLERGIASGEF
                     RRVDVAQTVPLIIAPLLQLVLWRQWPTDCMPAPTSDPQALLAQHADLLLHGLRPDTAT
                     P"
     gene            5485..6657
                     /locus_tag="PKN51_00035"
     CDS             5485..6657
                     /locus_tag="PKN51_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT83511.1"
                     /translation="MNPSFSRGLVRALLLLAVLAAVLLGWRGYQARQAKAPAPAAAAA
                     SAPVIELAATDLAHARQVALGTDIPITGTLKAVRSALVKARVPGELQGLTVREGDRVR
                     AGQVLARIEPTEYSERLRQTQQQADAARAQVDIAQRQYDNNAALVNQGFISRTALDNS
                     LASLEAARANHRAAQAGVEVVRKSLADTVLRAPINGQIAQRLAQPGERVATEARIVEI
                     VDASELELEAALSPADSVAVRVGQSAHLDIEGVTQPVAARVVRINPSAQPGSRSVLVY
                     LSVAPQPGLRQGLFAQGQLATGEQQALAVPLSAVRTDKPAPYVQVVAGERIAHRPVRL
                     GVRGRVDGEPWVQVEGVDAGATVLRGSAGVLREGTATRVPTPPAPASSAPAEAARS"
     gene            6816..10001
                     /locus_tag="PKN51_00040"
     CDS             6816..10001
                     /locus_tag="PKN51_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT83512.1"
                     /translation="MWFTKVSLNNPVFAAMVMLAFVVLGLFSLQRLQVDQFPNVDFPV
                     VVITTEYPGAAPTIVESEVTKKVEEAVNSVAGINALYSRSYENLSVVIVEFQLHIEGR
                     KAAEDVREKIGQVRPLLREEVKEPRVLRFDPASRAIWSVAVLPQASEGRAAPSPIELT
                     NWADQVLKKRLENVRGVGAVNLVGGAKREIHIQLRPAALEALGVTPEQVMAAVRGDNQ
                     DQPLGALRTPAQELTVQLQGRVERPEDFGRLIVARRGGAPIHLAQVADVRDGAQEQES
                     LALHNGERTLLLNVQKAQDENTIGVVDGLYRAVAEIRAELPPGVRLEPVIDQSRPIRV
                     AVQNVRQTLIEGAVLTVLIVFLFLNSWRSTVITGLTLPISIIGTFFFMQVLGFTVNMI
                     TLMALSLCVGLLIDDAIVVRENIVRHAQMGKRSYDAAMDGTQEIGLAVLATTLSIVAV
                     FLPIGFMQGIIGKFFHEFGLTIVAAVLISMFVSFTLDPMLSSVWHDPSIHAHGQRSGP
                     PVTLYERTIGRVTAWFDHASDRLADGYQRLLGWSLAHKPLTLLIAAAIFGLSVAMVRL
                     LGTEFVPKADFSETNVAFYTPQGSSLEATEAKARQVDALLRSYPEVRYTVATLNAGNA
                     QGKTNASLYIRLVDRHERTRNADDLAADFRQRLRAVPGITVTQAGLLEPVGGQKQIEF
                     SLQGPDQAELERLARPLMERLRDIPGLVDLDSSSKPDKPTVEVTVKRAAASELGLSSA
                     QIAAPLRTLIAGTTVGNWRAPDDQTYDVVVRLAADARSVPQDLERLPLTAGLNADGTP
                     RIVRLNQVATLNEASGTNQINRRAMLREIQITANTHGRATGEVSGDIRAALAQMPFPP
                     GYSYSFGGATKNMEESFTYALQALAMAIIFIYMILASQFKSFVQPLALMTSLPLTLIG
                     VVLALLMFGSAMSMFSIIGIVMLMGLVTKNAILLVDFAIRAREGRVSEGGVTEPLPRE
                     QALLAAARVRLRPILMTTLAMVFGMVPLAFAVSEGSEQRAPMGQAVIGGVITSSLLTL
                     VVVPVVYCYLDDLAGWLKRRLHRAPPDASAG"
     gene            10049..10723
                     /locus_tag="PKN51_00045"
     CDS             10049..10723
                     /locus_tag="PKN51_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNT83513.1"
                     /translation="MNVEQARFNMIEQQIRPWDVLDAHVLDLLAAVRREDFVPPAHRA
                     LAFADLEIPLKPGEQAVARGQVMLAPRVEARMLQDLQVAKHEKVLEIGTGSGFMAALL
                     GHRAQRVLSLEIDDELARTAHANLQRAGVLNVEVRALDGARTDLSAHGPFDVIVLSGS
                     VAELPDALLALLKPGGRLMAIVGHEPVMRATLVLRTDNGLVSSQPWDSNAPRLCHFPE
                     PSRFQF"
     gene            10757..11098
                     /locus_tag="PKN51_00050"
     CDS             10757..11098
                     /locus_tag="PKN51_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNT83514.1"
                     /translation="MIEQVSPRDLASWLRAAGGDRQPLLLDVREPQEWRLASVQIDGA
                     ELQQMAMHTIAPRLHELDPARPVAVLCHHGGRSMQVAMFLAQRGFARVANVAGGIDAW
                     ALQLDPALPRY"
     gene            11109..12455
                     /locus_tag="PKN51_00055"
     CDS             11109..12455
                     /locus_tag="PKN51_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNT83515.1"
                     /translation="MPLPFRRPRARLALLLLWGATTAQAQSLLQLYQTAHGYDATYRS
                     ALANAQASQARADQARAGLLPQLGMQAGAQHNWADTHVLGGGSSRSFNTLNAALVGSQ
                     PLYRPANRIAWDQGQRAAEAAQINLVGAEQDLIVRLAQAYFDVLAAQDALASVRALKA
                     AVNEQLQAARRNFEVGTATITDTREAQARFDLATAQEIATENDLRVRKLALDQLVGRS
                     GSEPRPLAQPITLPAPQPDDVTAWVERAAEQHPGVRQARAALDIARLETDKARTGHRP
                     TVDLQASVGQTRYPDGNPSLSAAPRASARTTNASIGVVLNWPLFAGFAVENRIRETLS
                     LEDRAQADLDQLQRSVSQATRAAFFGVQSGLGQVKALQAAEASSQTALQANQLGYQVG
                     VRINIDVLNAQSQLYQTQRDLARARYDVLVGTLRLKQAAGTLSQDDLIPINQLLVP"
     gene            complement(12741..13376)
                     /locus_tag="PKN51_00060"
     CDS             complement(12741..13376)
                     /locus_tag="PKN51_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83516.1"
                     /translation="MRNTLIGRFLLLSILWLALLLPLWYWAARWLAMPVIWLAGTVMQ
                     ALFPWVDGWAQEGVSAVLHTLVQVRMPGAQGDVLGELAPEASYPTYGYGLVLLWAMLL
                     ASRTERWWLKGLIGSLLLIPAQAWGICFQWLRDVVLMSGTYGAAYLKWSPLAQNAVAY
                     GYQFGFLMLTPVAPILLWLLFNKRFVAALWLEAALEAKPDPRAQAPTRSVE"
     gene            complement(13390..13908)
                     /gene="xrtH"
                     /locus_tag="PKN51_00065"
     CDS             complement(13390..13908)
                     /gene="xrtH"
                     /locus_tag="PKN51_00065"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HNT83517.1"
                     /translation="MKRFFLIFLTVQAVLFGIEMLNPVQTAVVHPWTGWLAKVSAAIV
                     HLIDPTVISYGRVLQSAKTGFGVSIEAGCNGVEATIVLVAAMLAFPSSWAMKAWGIGL
                     GFIAVQAVNLLRIVSLFFLGQWNFKVFEFAHLYMWQALIMLDVLVVWLLWMRYVAKRD
                     PQPAAGASAAAS"
     gene            14170..15831
                     /gene="ettA"
                     /locus_tag="PKN51_00070"
     CDS             14170..15831
                     /gene="ettA"
                     /locus_tag="PKN51_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNT83518.1"
                     /translation="MAQYVFSMNRVGKIVPPKRHILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGIDKDIEGEAVPMPGLDIGYLPQEPQLDPAHTVRQEVELAMGEVNAARA
                     RLEEIYAAYAEPDADFDALSEEQGKLEAVIAAAGTDSEHQLEIAADALRLPPWDAVIG
                     QLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEIFLKRFPGTVVAITHDR
                     YFLDNAAEWILELDRGQGIPWKGNYSTWLEQKEARLEQEKKSEDARMKAMKKELEWAR
                     QNPKGRQAKSKARLARFEELSDYEYQKRNETQEIFIPVAERLGTEVIEFKSVSKSFGD
                     RLLIDNLSFKMPAGAIVGIIGPNGAGKSTLFKLIAGREQPDSGEVVIGKTVQLAFVDQ
                     SREALADDKTVWEDVSGGLDLITVGKFQMPSRAYCGRFNFNGQDQQKKVGMLSGGERG
                     RLHLAKTLMQGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLDRICT
                     HILAAEGDSQWVFFDGNYQEYEADKIKRLGEEGARPHRVRYKALK"
     gene            15863..16519
                     /locus_tag="PKN51_00075"
     CDS             15863..16519
                     /locus_tag="PKN51_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83519.1"
                     /translation="MGTPATLIVTGFDAQHFDLAQDFADSFRQAYQEQYQLAAVVFGP
                     QAPPPQLSDRFDRITRLDASGPSFDAGRNGYFLAHAGVNARLRDIFPGYASYCWVDAD
                     CWFQGDESLPRILAGMPGFDLCIHPEFDVHYLNFPTPSERTLHIYRTNEAANLSAMPL
                     RMPMVNAGVFAMRTDSRVWALWDAELTGLRQRHERGEAVFFSDQIALHKLVYLNQLRI
                     "
     gene            16595..16726
                     /locus_tag="PKN51_00080"
     CDS             16595..16726
                     /locus_tag="PKN51_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83520.1"
                     /translation="MPTPPHEKIGVMHLAGRSKHQQLQIDGQTLTLRYRDMARLFAA"
     gene            complement(16821..17009)
                     /locus_tag="PKN51_00085"
     CDS             complement(16821..17009)
                     /locus_tag="PKN51_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNT83521.1"
                     /translation="MKNVGGIDRILRIVVGLVLVGLAATGSVGWWGWIGVVPLLTGLL
                     GSCPAYKLLGMNTCPMKK"
     gene            complement(17207..17554)
                     /locus_tag="PKN51_00090"
     CDS             complement(17207..17554)
                     /locus_tag="PKN51_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83522.1"
                     /translation="MYEIWLMLNIVYELALSIWPWLLALAALWLLLMGRAWGRRGADW
                     RASLPGAIVLGLLVAIVIFAVTPIWNKSALGEMKYWVDWANLLGIAAAWGVAGVAFGW
                     PLLTGLRRSGKAG"
     gene            complement(17582..18916)
                     /locus_tag="PKN51_00095"
     CDS             complement(17582..18916)
                     /locus_tag="PKN51_00095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNT83523.1"
                     /translation="MTHRSDQRPSRRDLLRTLGAAPLAASALQAAPTARAQTSSSQAR
                     IVIAGSGLGGIAVASRLRQLVPGAKITIVDAKQEHNYQPGYTLVATGIWPIEKVKNRN
                     AEFQPEGVEWVQEMVASFDPASNSLTTTGGRKIGYDFLVVATGLQLGYDQIEGMDVKA
                     IGSNGLGSVYASPEAALATWQAMDAFRAKGGQAVMTLPAGPLKCAGAPLKMTFMVADR
                     LRQAGTLDKSKVDFFSGLPDDTVFGVKPVNDNVLARWKALGIQTHYLRKLVAIDPGAR
                     KAFFTSPEGERQEVGYDFLHVVPPMRAPDAVKGSDLAAKEGPMAAGGWLEVSKDTLQH
                     KRYPNVFGVGDINGTPRGKTAATVKKSAPLVAHNLVRVIDGQPPDQIFDGYTSCPMIV
                     REGSAMLIEFDYDGKLTPSLPMIEPLQESFMAWFMKYRLLKPAYIAVLKGRV"
     gene            complement(18944..19648)
                     /locus_tag="PKN51_00100"
     CDS             complement(18944..19648)
                     /locus_tag="PKN51_00100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT83524.1"
                     /translation="MDWSQARIERDELNLLAELVPLAGLQVIELGCGSARLARDLLAR
                     HPDARVTGLEVDAIQHARNLAAPAERLSFIAAGAEAIPLPEAQFDLALMLKSLHHVPL
                     ALMDQALREVHRVLRTGGHLYVSEPVYAGALNQITRLYNDEGAVRAAAQAALDRALAS
                     GQWEAAAERHFAVPVRYADFADFERRMLDVTYAERHLDDDLREQVRARFAAHVGPEGA
                     HFQRPMHVRLLRKAGA"
     gene            complement(19660..20802)
                     /locus_tag="PKN51_00105"
     CDS             complement(19660..20802)
                     /locus_tag="PKN51_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT83525.1"
                     /translation="MNGVAAPQIVVLGAGFGALSTVRSLRQQGCTAPITLVAPRAELH
                     YLPGIIWIPSGLRTRADLVVPLDNFFARMNVRHHLASVTGLSDDGRVVHTSAGELANE
                     ALVIASGARFIRKLPGIEHAITPCEGIDAAERIRDRLRQMSGGTIAVGFGANPNEPSA
                     VRGGPMFEFLFGIDRQLRREGRRERFQLVFFNPSQQPGARLGSRTVARLLAMMAQRGI
                     QTHLGHKMVRFEADRVVTEGGEFAADLILFMPGLTGPAWLEQSSLPRSPGGLIAADAG
                     CRVPQLERVYVVGDSGSFPGPDWMPKQAHMADLQAEAAARNLLSELAGQPGTQGFKVE
                     LLCIIDALEEGTLVLRTERRQIVLPPTRLMHWAKRSFERRYLARYR"
     gene            complement(20799..22172)
                     /locus_tag="PKN51_00110"
     CDS             complement(20799..22172)
                     /locus_tag="PKN51_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT83526.1"
                     /translation="MKRLTRLVLVGAALGVPVGAWAQDLLTVWRAAAGHDQRLAVARA
                     EHGASQTLRAQADALWRPQVGLNLGLGLGVQDTAMRGARFSAPGMGQVDEARFATSVT
                     GGLATRVAVQAQQPLVNATRQAQQAQLRLGADMGDIAWRGAQADLMLRTTERYLALAV
                     AQERVRVVERQWQAVSRSRAEAHDRYQLGASPITDTHEADAALAGVRAQQSAAELELS
                     VQRDRLAGSTGLAQPRARLPLAPLAPVEALPFWLAAAESDNPRLRLLAQAVALAEQKL
                     RQHSVAGRATLDLVAQAGHERIAGGGDFGSARNRSLNGMVGVQLNIPLYTGGMREAQE
                     REAAERLNQAMAELELAREDVAQQVRATWQGLQTGAARLAALQEGLVASRARLDATRL
                     GREVGDRTTLDVLNAENAQADAELALAQARSDQVLTRLRLAALADRLDEALLTQVDAT
                     LAPEVPR"
     gene            complement(22169..22525)
                     /locus_tag="PKN51_00115"
     CDS             complement(22169..22525)
                     /locus_tag="PKN51_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNT83527.1"
                     /translation="MVENYTNLIAAVSANSAKLRKDIPEVMQGFNAMAQAASKAGALD
                     AKTKELIAMALSVGARCDPCVGYHAKALVKLGATRAEVEEMLGMCVYMGGGPGLMYAA
                     SALAAYEEFGGEKASA"
     gene            complement(22557..22772)
                     /locus_tag="PKN51_00120"
     CDS             complement(22557..22772)
                     /locus_tag="PKN51_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNT83528.1"
                     /translation="MTSWRIVRIFAGTFILLSLALGVQGSPIFHSSNWLWFTAFVGAN
                     LLQSGFTKWCLLNNILLKLGVPAESCS"
     gene            complement(22788..26093)
                     /locus_tag="PKN51_00125"
     CDS             complement(22788..26093)
                     /locus_tag="PKN51_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT83529.1"
                     /translation="MGQPLHADRAPRLGVSGRIAAYFQSAQITPLLALVAFLLGLFAV
                     LVTPREEEPQIDVTMANVIVPFPGASARDVEAMVATPAEHVLSQMSDVEHVMSVSRPG
                     VAVLTVQFKVGVPRQTAVVRLYDAVGANADWLPRGLGVLPPIVKPKGVDDVPIVALTL
                     SSARQDSGAFDLERVAHSLESELKRVPGTREVYTLGGPGRAVMVEADPQRLAAAGVSL
                     HELHGALQSANLGLPVGELLGGNRALAVETGAYLHSAQDVAELVLAVRGGKPVYLREV
                     AQVRDGAPPPTRYVWHGEVRRGDGGAPATALEQPAVTLAITKKPGENAIDVADAVLRR
                     VDQLRNTVLPADVQVTETRNYGATANDKATTLIKKLLFATLSVVALVFFALGRREAAI
                     VGVAVILTLTVTLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHQSLYPE
                     RTLAQIIPGAVDEVGGPTILATFTVIAALLPMAFVSGLMGPYMSPIPINASMGMLLSL
                     AIAFSVTPWLARLWMKATHQADHGQAAGHGLAARLTPLFERIFRPLLDERRGARHRRL
                     MGLAVAGLIGLSVALPVLGLVVLKMLPFDNKSEFQVVVDMPAGTPVEKTAAVLRELGA
                     HLASVPEVTHYQAYAGTAAPINFNGLVRQYYLRAGGEVGDLQVNLVDKAQRKDQSHAI
                     AVRERAALRAIGQRHGANVKVVEVPPGPPVLAPIVAEIYGPDADGRRAVAQQVRAVFE
                     ATPGVVDVDDTTIVDAPRTVLVVDRRKAAQLGVSQQAIASTLRMGLTGDAATYLHDAS
                     RFAVPAFIQLPAELHGSLDALLQLGVRSASGALVPIRELVTPSNTLREQPIYHKDLLP
                     VSFVMGDMAGQRAGELDSPLYGMFQMRGALAELKMPDGGRLDEYFIRQPQDPYRGYAL
                     KWDGEWQITYETFRDMGAAYAVGLVLIYLLVVAHFGSYLTPLIIMAPIPLTIIGVMPG
                     HALLGAQFTATSMIGMIALAGIIVRNSILLVDFIKLQVSEGMPLARAIVQSAIIRAQP
                     IGLTALAAMIGALFILDDPIFNGLAISLIFGILVSTVLTLVVIPILYFAAYRRTHEKN
                     SALAPTQPAQAAT"
     gene            complement(26093..27115)
                     /locus_tag="PKN51_00130"
     CDS             complement(26093..27115)
                     /locus_tag="PKN51_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT83530.1"
                     /translation="MLLLAACGALVLPVSAQELPHMTVNAQAARSAQAFEGTVEAVRQ
                     SVLAAQVPGAVLELNVKAGDRVRAGQTLVRIDARAAEQGAAASQAQVVAARAALEVAA
                     AELARKRQLYERNYIAKAALEQAEAAQRAAQAQVNALQAQAGAARTQTAFHVVTAPFD
                     GVVAAVSVERGSMAMPGSPLLTLYDPAALRVSAAVPSAVVAAGVQQARIELDRARAPI
                     HPTRVQVLPTVDPGTLTQQVRAELPVGLEGVAPGLFARLWLPGTGGADAAAPQRLFVP
                     RTALVQRAEMTGLYVLGEQGQPLLRQVRLGPVQGDQVEVLAGLSAGDKVVTAPQAAVR
                     LAPAGR"
     gene            27288..27650
                     /locus_tag="PKN51_00135"
     CDS             27288..27650
                     /locus_tag="PKN51_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT83531.1"
                     /translation="MQQLPPEALEHVAHYFRTLSEATRLRILNVLGHGEMSVGDIAEK
                     VDSSVANVSRHLAQMAQHGLVARESRGNSAYYRIADPAVHQLCDLVCGNIARRFEDVA
                     STRAAFVRTARAKPTTGR"
     gene            complement(27663..28103)
                     /locus_tag="PKN51_00140"
     CDS             complement(27663..28103)
                     /locus_tag="PKN51_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT83532.1"
                     /translation="MYKHILVPVDGSTASMAAIDKAAGLARAYGVPVTAIFVIDPYPF
                     TGVGADFAYGQDQYLNAARAEAGAAIEAANQRLQAAGVQVDSRVVESHAVWRGILEAA
                     GAVGADLIVMGSHGRRGLEKLVLGSVAQSVLSHTKIDALVVRAS"
     gene            28295..28948
                     /locus_tag="PKN51_00145"
     CDS             28295..28948
                     /locus_tag="PKN51_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83533.1"
                     /translation="MPMDTINLALVGISLGLALSFRPWRLLAGGALLSPLLASLVITP
                     WLWALPWLHRMPIQLQLSGACLIGLALGWPLAVPVFCLIAVLVGLIAPVSWAQQLDMA
                     LWLGIVPATLSFGLGMVLRRWVWRNLFVYILGRAFLGTALCMFASGALAQWTGHRLAA
                     AVDPDLAMVARWLIAWGDAFMTGMMAAICVAFRPHWLATWSDWLYIAPPPSPRSNQP"
     gene            29028..29165
                     /locus_tag="PKN51_00150"
     CDS             29028..29165
                     /locus_tag="PKN51_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HNT83534.1"
                     /translation="MKLWQDLFGTDYGLMSIAGIIFMIGMAIWFVRFFLRKIAQSSDQ
                     Q"
     gene            complement(29181..29771)
                     /locus_tag="PKN51_00155"
     CDS             complement(29181..29771)
                     /locus_tag="PKN51_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT83535.1"
                     /translation="MSAPAAAAKVPTPPEARARLTSRPGDAPVQAALSAQVPAPRRAA
                     EQRRLAALQARGDDAHAYLVETALRICRMPLGAMALRDGPWLRFKAQAGVAVEALPLP
                     GSPCELAMRCADQVTVIEDLGGDPRFSGCALLAQGLAIRTWAGAPLVGASGKVWGVLW
                     VADRRAGPLSPVQLRTLQLLARQTALLLEAAEAPRG"
     gene            29916..32318
                     /locus_tag="PKN51_00160"
     CDS             29916..32318
                     /locus_tag="PKN51_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNT83536.1"
                     /translation="MGLRLLRHMAIGLAGMLALGAMAAPAFDQVRADFRPSETVLLDR
                     SGQPLHRLRTDARVRRGDWVRLDQVSPALRQALVLSEDKRFYEHSGVDWHAVGAAAWA
                     NLWHTRTRGASTLTMQLAGLLDDDLRLGSGGRSLRQKVGQVVLATQLEAQWRKDQILE
                     AYLNLVPWRGELVGIDALSRTLFAKAPHGLQDGEAAIAAALVRAPNAPAAQVARRACG
                     VLQSMRGDRAAADCVALDMQTEAALARRAFAPSDGIAPHFARRLLAATRNGSDRAPAT
                     LPSTLDGRLQRLALEALQGQLRELRGRHVADGAVIVLDNASGEVLAWVGSSGALSSAA
                     EVDGVLAPRQPGSTLKPFLYAQAIAERRITAASLLHDSPAQLHTASGLYIPRNYDLQF
                     KGWVSARTALAASLNVPAVRVGVMVSPRAFAQQLGRLGIELAESGDYYGYSLALGSAE
                     VSLLQLTNAYRALANGGRLCPVRLLPNAPGSLPLPLGEGRGETETHSRAASLSLPLPS
                     VGEGRGEGNRSPQVTATTPCTQALDAGAAFITSDILSDRNARAPTFGTDSILATRFWS
                     AVKTGTSKDMRDNWAVGYSRRYTVGVWVGNASGAAMWDVSGTSGAAPVWATLMRELHR
                     HTPSPPPAPPAGLQHVAVHYAAGLQAPRSEWFLPGTAQSEFAIETEAASADPAKAGGQ
                     KGLKTASHPSAPAGVPPRITAPQDGTILALDPDIPPAHQRLRLAAEPGSAPANSLRWW
                     VDDQPIGRGAQADWLPMPGRHRITLCDGRGRVVDERRIEVRGAQPRHPRSAGAADRLA
                     KQ"
     gene            32338..33795
                     /locus_tag="PKN51_00165"
     CDS             32338..33795
                     /locus_tag="PKN51_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNT83537.1"
                     /translation="MFEQTFKNIDDILHKDAGCTSELDYTEQSSWLLFLKYLDDLEAD
                     KAAEAELAGKPYRYILDAPYRWSSWAAPQDAAGALDHNSALTGDDLRDFVNQRLFPYL
                     AGFKARASGPNTIEYKIGEVFGEIKNKLQSGYTLRDVIEQVDALRFRSQTEKHELSHL
                     YEAKIRNMGNAGRNGGEYYTPRPLIRAMIQVTKPRIGERIYDGACGSAGFLCEAFDYL
                     RSQPGLTTADLQTLQQRSFYGKEKKSLAYVIAIMNMILHGIEAPNIIHTNTLAENLAD
                     IQDQDRYDIVLANPPFGGKERKEVQQNFPIKTGETAFLFLQHFIKSLKAGGRAAIVIK
                     NTFLSNTDNASVSLRKLLLEGCNLHTVLDCPGGTFQGAGVKTVVLFFEKGAPTRSIWY
                     YQLDPGRSLGKTNPLNDADLAEFVTLQASFADSAQSWSVDASRIDAASCDLSVRNPNG
                     GEAVALRSPHEILDEIAALDAESAEALARIRELVA"
     gene            33792..35033
                     /locus_tag="PKN51_00170"
     CDS             33792..35033
                     /locus_tag="PKN51_00170"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNT83538.1"
                     /translation="MNGRWPVRPLGSLCRIARGGSPRPIQAFLTDTDDGINWIKISDA
                     TASGKYIYSTVEKIKPSGVSRSRMVKEGDFLLSNSMSFGRPYIMRTMGCIHDGWLVLS
                     GYQNFFDQDFLYHLLGSPQVFQQFNQLAAGSTVRNLNIDLASRVQLPIPSLPEQRRIV
                     AILDEAFDAIATAKANTEANWQGARAVLNQCRDDALSSLAAEFGTVNLGSVCDFENGD
                     RGKNYPGKQHRVAEGVPFINAGHLTEDGIDFESMDYISPERYSLLGNGKIKPRDILFC
                     LRGSLGKFANVGDTPVGAIASSLVILRPRSPLYDKYLEIYLASSCCIEMINKYKGGAA
                     QPNLGAKDLKQFVLPLPPQSLQRQLTERLDGIKEQTHLLEAIYSEKSAALDELKKSLL
                     HLAFTGQLTARAADRQLQAVA"
     gene            35030..37336
                     /locus_tag="PKN51_00175"
     CDS             35030..37336
                     /locus_tag="PKN51_00175"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNT83539.1"
                     /translation="MNEAETRAEHIDPALAAAGWGVVEGSRIRREVIAPGRLQGRGQR
                     ARADIADYVLIYRNTKLAVVEAKAWDKPHTEGLAQAKRYAAKLALRFAYATNGQQIYG
                     VDMATGAEGDVPAFPGPDALWAQTFAQANAWRDRFAAVPFEDKGGAWESRYYQEIAIR
                     RVLEAIAGGQSRLLLTLATGTGKTAIAFQIAWKLYHSRWNLGDWQAPGAPTRRPRILF
                     LADRNILADQAYNAFSAFADDALVRIAPGEIRKKGRVPRNGSVFFTIFQTFMSGPGGA
                     PHFGAYPPDFFDFIVIDECHRGGASDESQWRAILEHFAPAVQLGLTATPRRRSNADTY
                     AYFGEPVYTYSLKEGINDGYLTPFKVRQFATTLDDYTYTPDDAVVEGEVVPGKRYVEG
                     EFNRVIVIREREQYRVGLFMGMIDPREKTLVFCATQEHALMVRDLINQAKTSPDPNYC
                     MRVTADDGAMGEQFLRDFQDNEKSIPTILTTSQKLSTGVDARNVRNIVLMRPVASMIE
                     FKQIIGRGTRLFDGKDYFTIYDFVKAHHHFSDPEWDGEPVEPEPPRPRAPAPTADPGE
                     PPAVTEVPPAYRIRIKLADGKARAIQSMTATSFWGADGRPLSAAQFLEMLFGALPEFF
                     KDEDELRALWSDPATRRRLLDQLAERGFGREPLAEMQKIIEAEHSDLFDVLAYVAFAT
                     QPRTRAQRADAAKAAAAPGLSDKQQAFVDFVLAQYVQQGVDELDQDKLSPLLRLKYRA
                     LPDAFAELGRPEQIRGVFVDLQRHLYLD"
     gene            37470..37772
                     /locus_tag="PKN51_00180"
     CDS             37470..37772
                     /locus_tag="PKN51_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNT83540.1"
                     /translation="MRVIAVSTLRAFWQRHPDAEQPLNAWYEEATSATWTQPADIKAR
                     YRSASVLKNRRVVFNIKGNDYRLIVAIAYRLQIVYVKFVGTHQEYDAVDAETIESA"
     gene            37791..38162
                     /locus_tag="PKN51_00185"
     CDS             37791..38162
                     /locus_tag="PKN51_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNT83541.1"
                     /translation="MDIRPIRTEADYQATLKEISGLMACDPELGTPEGDRLDILATLV
                     QAYEAKHFAIGAPDPVEAIKFRMEQSGLSVKDLEPLIGKSNRVYEVLNRKRPLTLAMI
                     RRLHQSLGIPADVLIAETAAG"
     gene            complement(38435..39187)
                     /locus_tag="PKN51_00190"
     CDS             complement(38435..39187)
                     /locus_tag="PKN51_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT83542.1"
                     /translation="MDRSTAGAQSLRRGLQLLRLLAEHQEDGIRLSEAMAASGLQRST
                     CHRLLACLVEEQFAERDAGGKVYRLGVDAMQLGFAAMRRVPLVDQCRALMQRLARMSG
                     DTVFLVIRQGDHCLCLHREEGPFPVKVFTTDIGGRRLLGLGAGGLAIMARLPDAEVLR
                     IYERNAAEYAQADFTRERLLRAVHQTRAQGWADITDTLTAGVSGVGVTFAAAPGAIAA
                     LSFGAITPRLPIERKRELARLLRQECPAVAGG"
     gene            39283..40086
                     /locus_tag="PKN51_00195"
     CDS             39283..40086
                     /locus_tag="PKN51_00195"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNT83543.1"
                     /translation="MAFDTLAIETHGAVRRILLARPAQRNAQNWQLLDELMAALDQAR
                     TDDAIHAVVLGGQGEHFSAGHDLKQAQAERANFTVEQRWAFEELRYFDYSMKIWDFPK
                     PTIAQVQGACVAGGFMIANMCDLVVAGESAYFSDPVGHTLGAAATEVLIHPWVMGARK
                     AKELLFTGARMSAQEAQAIGMVNRVVPDAELADATLALAQQIAKAPPFGLRLIKRSIN
                     RTLDAQGLRSALAAHFDTHQLSHLSDGFLSARDRGLASAIQKNPAKAQQ"
     gene            40092..42179
                     /locus_tag="PKN51_00200"
     CDS             40092..42179
                     /locus_tag="PKN51_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNT83544.1"
                     /translation="MHRLQPLLDPASIAIVGASNNPARIGGMPIAHLTKFGYAGAIYP
                     INPKYQEVFGLRCWPDIEALPAAPDLVVLAIAAPEVLPMLRRCAAKGVRAVIVYAAGF
                     AEEGERGAALQAELAAYVASTDMVVAGPNAMGFANLNTQAHTNFASVFNTAPMQAGTG
                     SVSLLTQSGNVCAAVYAIARRLGVAFSHFINTGNEACVDFAQYLEYLAQDEATDVCIG
                     YIEELRDGARFIAAAEEFARRDKLLILYKAGETDKGSEAVRSHTSALAGDQAIYRAAF
                     RQLDIIQSHDFVQMAQLAHLASYRRRRAGKRVAIITISGALGAILADKFIGAGLEVPT
                     LPAELQQQLRAGIPDYGMVANPVDVTGNVVNDPGFVRTALEALATSDAVDTVVIYAPG
                     YMLDRMAGDLCAVAAAHPRLFVAIDTGAAQSREQLRAGGVPVFEDIGVATAALAPFLL
                     WQQQRQRNRWRALRHQAPPPATALPEVLDEHQCKAVLAASGVPAAPERRARSADEAVA
                     AADALGYPVALKVLSPDIAHKTEAGGVRLHLADGAQVRAAFDQILASARAHAPQARLD
                     GVLVAPMQSGVAELIVGATRDPVFGMALTVGLGGVLTELYQDVSHRLLPVDEAMAADM
                     LAELKAYPLLTGYRGRPPADVDAACAAIAAFSRAVQALGRQVSEAEVNPLLIKASGQG
                     AAMLDALLLAPAD"
     gene            42214..43179
                     /locus_tag="PKN51_00205"
     CDS             42214..43179
                     /locus_tag="PKN51_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT83545.1"
                     /translation="MIKQLLAGVALAAAALCTPAQAQGYPSKPVNIVVPFAPGGATDI
                     MTRLLAERLSKRLGQPVIVENKPGAGTMIASEYVAKAPADGHTVLMAASSLGIAPSLY
                     SKVNYDPVKSFAPISLVASVVHVLEVHPSVPVKSVGELITWLKANPGKANYGSVGAGT
                     STHLESELFNTMAGVKMVHVPYKGSAPALTDLVGGSLQVMFDAWASSGPFVKDGKTRL
                     LAVTTAQRSRILPDVPTVAESGLPGYEAMPWLGLLAPAGTPDAVVRKFHDELIEVLKE
                     QPVQEKFHSLGLDIIGNTPQEFAEFIKKDITKWAKVIQETGAKAN"
     gene            43258..>43881
                     /locus_tag="PKN51_00210"
     CDS             43258..>43881
                     /locus_tag="PKN51_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT83546.1"
                     /translation="MQVGYAAEDEAFRAELRAWFETHYPRFVAAWPQAPDPNALDWRR
                     AWEDYVCQAGWSGLGWPLAYGGKAWSLTRQAIFHEEQARIGAPLGVNLIGHGILAPTL
                     IHFASEAQKQRYLPGILSNREVWCQGYSEPGAGSDLAALGTRAERRGDHYLLNGHKIW
                     TSFAHIADFCFVLARTDPAAQRHKGISFLLVDMKSPGVRVQPIRQITG"
     assembly_gap    43882..43981
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <43982..44259
                     /locus_tag="PKN51_00215"
     CDS             <43982..44259
                     /locus_tag="PKN51_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT83547.1"
                     /translation="RALTLALRGGPPGAEGSATKLHWSEAHQRLTSTAAELLGERALA
                     GPTPGGHDPAAALWAREFLWTRAETILAGTSEVQRNILAEQMLGLPR"
     gene            44264..45325
                     /locus_tag="PKN51_00220"
     CDS             44264..45325
                     /locus_tag="PKN51_00220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT83548.1"
                     /translation="MDFALNEAQQMLQASARRFLADNHPLTRARQALPWSDAAQQRLW
                     ADMADMGFLALLLPEDQGGLALGLTEASLLAEEAGHQLLNLPWAASAVWLALACRATP
                     ASVPAPLRDAMQAMLAGRRALHVVDAGDGWWDHAGQCSDIVVIRESPAPGEPLQLALI
                     DGAAVTAAPALDPTLRQAPALAPTGPWHAWALDAASRAQARTGYRLMRAAELVGVAQA
                     ALELAAGYARERVQFGRPIGSNQAIKHQLANAWMGVDNARLAVLYATAALNERLPDAR
                     LACAAAEASAIEGALQATRSALQVHGGMGFTWESDVHLYLKRAQHLATRLGGASRALQ
                     QLEALAWDAAANAPSATPV"
     gene            complement(45546..46307)
                     /locus_tag="PKN51_00225"
     CDS             complement(45546..46307)
                     /locus_tag="PKN51_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNT83549.1"
                     /translation="MNNTLIKGLRLLEVLVARAQPVGISELAQELEMGASNVHRLLQA
                     LVELGYAVNEGGRGGYRASLKVWELGAQALHKLDFRETAAPAMRMLLAETNETVHLSV
                     LSGDEVIYIDKLDSPEPVRAYSVIGGRAPAYCVATGKALLAWREQPAVQLMTVRPLQV
                     YTPTTLADTSALAMELDRVRQQGYAVNRGEWRASVWGIAAPVFDERQRVVAALGISGP
                     AERIRARGVKRLAELVVQAARQASSRQLALAGEAR"
     gene            complement(46536..47363)
                     /locus_tag="PKN51_00230"
     CDS             complement(46536..47363)
                     /locus_tag="PKN51_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT83550.1"
                     /translation="MLWKTLKVPEIETLPGPVFVRAQDIPPGYRFPSHSHPWHQLIYA
                     ISGSLVVEAAHQRVVCGPEQAVWIPTGTVHAVASRYGAQFRSLYLRTSVGRGMAARYA
                     LLQVSALLRELIREAAWLGEQRVAGVATLAYTQRVHGLILDQLPRLERVQAPLPWPQS
                     PMLRKLCEAIDRAPADADDIATWGARLGASERTLTRRFQAELGMNFREWKRRLRLFKS
                     MELLGAGMSVTATALELGYGSPSAFSYMFTREMGCSPKTYRAAPGRERQRSARSRAR"
     gene            47494..48858
                     /locus_tag="PKN51_00235"
     CDS             47494..48858
                     /locus_tag="PKN51_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT83551.1"
                     /translation="MPAHIEPPRRIGFAATALFIYLILLAGYVLSYVHRTAPAAIAGE
                     LTQAFQVSGALLGTLAATYFYVYTVLQVPVGVLADTVGPRVVVTAGAVVAAVGSIMFG
                     LAPGWELAAVGRLLVGMGVAVTFVALLKVCANWFPPQRFATFNGITLLAGNLGAAAAG
                     APLAWLVTVASWRSVFVGLGLASLALAVLTWIVVRDSPRECGFEVSYAPPPAVATHWA
                     QALRAVLANPASWPGFFVNVGVAGSFFAFAGLWVVPYLREGRGLSQAVASHHASLLVL
                     GVAAGSLVVGLLSDRLRSRIGVMRLFALMYALSWLPLVLRWEPSLALSYAWFFVMGLF
                     VPAFVLTWTVAKEMNPPEHAGMAVSLVNVGIFLGAGILQPLAGALLDAVRPVLGPTGA
                     WDRAIWLFAASAAAGALCTLFVRHEYPAPAPAHSRRQTGAAATAARPGAGHGSVCHSK
                     RGNP"
     gene            48978..51317
                     /locus_tag="PKN51_00240"
     CDS             48978..51317
                     /locus_tag="PKN51_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HNT83552.1"
                     /translation="MTASHWGVYEATGQGEALRLQGFARDPSPSPIGLSMLDAARGPV
                     RVRRPAVRRSWWQAMRGDAPHAAPQLRGTDPFVEVGWNEAIDLVARELDRVRLRHGNE
                     AIFGGSYGWSSAGRFHHAQSQVHRFLNAIGGYVRHADTYSLGAARVLLPHIVAPMEEL
                     FTQHTSWRVMARHTRLFITFGGVPAKNAQVSSGGATEHAVPTGLRRMAAAGVRFINIS
                     PVRDDIDTGGEVEWIPIRPGTDTALLLAMAHTLWTEKRHDAGFLASHCVGFERVAAYL
                     RGDTDGQPKSADWAARITGVPAERIVRLAREAAATRTMLNIAWSLQRSHHGEQPYWAL
                     ITVAAMLGQIGLPGGGFGVGYGSTNMMGNDNAKFSGPTLPQGHNAVRAFIPVARITDM
                     LEQPGASFDYNGQRHTYPDIRLIYWAGGNPFHHHQDLNRLLAAWQSKPETIVVHEQYW
                     TPTAKLADIVLPATTAMEREDIGYATRERYMVAMKRLIAPVGEARDDYAIFSDLAERL
                     GVREAFTEGRDARDWLSLMYEQSRQRAAQAGIELPPFDDFWTRGLLDLAQPEAEVVML
                     DRFRADPQSHPLATPSGRIELFSERIASFGYDDCPGHACWLEPLEWLGSPAAVRFPLH
                     LISDQPHTKLHSQLDHAACSQANKVDGREPLTLCRIDAEARGLKAGDLVRVFNARGAC
                     LAALRISDALRPGVARMSTGAWFDPTSWLASGPGNVERHGNPNVLTPDIGASRLSQGC
                     MAQTCLVEVERCTSPETRPTPYLLPEFVGTAHPHEPACT"
     gene            51366..52967
                     /locus_tag="PKN51_00245"
     CDS             51366..52967
                     /locus_tag="PKN51_00245"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HNT83553.1"
                     /translation="MNTPVCDYLIVGGGTAGCVLANRLSADPSCRVILLEAGGPDDDK
                     WIHIPAGIRYLLREKKHNWFYMTEPSPLTNGRSVYWPRGKVLGGSSSINGMVYIRGQR
                     TDFDRWAASGATGWDWDSLFPYFLKIENQTRGADAYHAVDGPLTVSDRTNRSEVWTRF
                     IAAAEQLGIPRNPDFNGADQEGVGYYQATVGNGRRCSAAVGYLRPVKGRANLSVITGA
                     MVHHISFEQRRATGVRYEKDGHWHELRAAREVLVCGGSINSPQLLMLSGIGPAAHLQE
                     MGITPRVDAPQLGKNLQDHLQLRLTYRLNRPISFNDQFHSLAGKVGMGLEYLLRRSGA
                     IAYPTAQVGLFTRSLPELASPDIQYHFSNYTTDEKTGMPDRFPGMTFSVCHLRPESRG
                     EILLRSPDPGQHPRILPNYLATPEDCRVAVEEVRLTRRLAATQPLADIVAEELAPGPD
                     VQSDEACLDFARSNGTSIYHPVGTCRMGSDAEAVVDPALCVRGVSHLRVVDASVMPTL
                     ISGNTYAATLAIAERAADLILNTRP"
     gene            53003..53965
                     /locus_tag="PKN51_00250"
     CDS             53003..53965
                     /locus_tag="PKN51_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT83554.1"
                     /translation="MKLTPILTGLSACLMAAAAHAQSVDKYPERPIKIIIPYQAGGGT
                     DVMTRIIVEKVRERTKQPFIIETKPGGNTVIGVKALLAAPADGYTLMMTSSATFHTAP
                     IIVKNPGYDPIESFSLISYIAYTPVVFVINSSVKANNLKEFIDLAKAAPGKYALSTYG
                     RQLGTDLFQKVAGFKLNSIPYKGVEAVNAVVAGHTDAMFDGVFTALPYLKQGKTRALA
                     VMQDKRTRFAPDIPTVTELGYKSMPDIPIWYGLVGPKGVDQKIIDKLANEFKIAMESP
                     EVIEKLANLSVESVGSGPQALRKQITAEYGHYRKIADELGIQPE"
     gene            54009..54989
                     /locus_tag="PKN51_00255"
     CDS             54009..54989
                     /locus_tag="PKN51_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT83555.1"
                     /translation="MTHPDRLRLSLPLLLAAPQLLWSVPARAQDKWPSGPIRMVVPFP
                     PGGSSDMLGRLMADKLSALLKSNIFVENKPGGTTQIGTELVANAAPDGHTLLMASATG
                     FTALPNLRKLNYGIDSFEIAGGVADYVAVMAVRKTLPIRTVKEFIDYAKQHPGKLSFG
                     SAGEASAGHIYGLTLARDTGIQVLHVPFRGSAAAVNALVAGDVDFIIDGAVTPMVKAD
                     RVQPLAVLYRRRHPELPGVPTLEEAGFTISSTRGSGWGVLAPKGTPAAIMSRLSEAIR
                     QVLQQQDVKDALARANSVPSWQAPDEYRRSLDADRKLYAELLPAIGIRGN"
     gene            55000..57129
                     /locus_tag="PKN51_00260"
     CDS             55000..57129
                     /locus_tag="PKN51_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNT83556.1"
                     /translation="MVDVSNPAAWVAPRQSVRDILHPRSVAIVGASDSEDKWGGRLLR
                     YMLRHRHPGALYPINARATELMGLPAYPSLRDCPGPVDLAILLVPRAHVRAAIEDCVA
                     KGVGCALCITAGFAETGAAGRADEQALVDLARAGGVRLIGPNCMGLMNTHHGLCATTG
                     VVMGTLDRLPVGGIGMASQSGALMGAMISRGVDIGAGFSTTVSVGNQADLDLNDFFEY
                     LIDDPKTEVITLYMEGVKDAPRFTALLERAAAAGKPVCIAKSGRSAAGAKAAASHTAS
                     LAGAWPAFEATCRRWGVYLFETIYDLLQGALLLQRGKRAASDQVAVFSGSGGGGALLT
                     DGLEAHGLAPAELSDATQQAVAAVLPSTHRQLPFDFGMLNHAAAPDPQVAGGAIGIAL
                     DRALHDPAVGAGILLLTTQPGQELVVQAAIAAADRSSKPLLFVQGAGNHGDVARQMLR
                     AAGHGYFDSPHDALKVLSVLARRKPPPPSAPPQPGVSVAADLPAGFLTEPQARRLLEG
                     AGIPTTRWRFAAGADAAAQAARELGGSVAIKAVSAHIVHKSDMGGVKLGLQGDAAVRQ
                     ACADIAAAARAAGVDTLDGYLVTEMWRADTELILGVQRDPDFGPLVMVGAGGVLVELM
                     KDVQLAPAPLSHATALAMLRQLRCLPLLSGYRGRTPADLDAIADVMVRLGALAADPSA
                     RLRELDINPLFIAGPRIAAADARATLA"
     gene            57167..57907
                     /locus_tag="PKN51_00265"
     CDS             57167..57907
                     /locus_tag="PKN51_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNT83557.1"
                     /translation="MTAVRYASEGGIARITIHRPEARNAINDEVVQGMQAAWQRFAQD
                     ADDRVAVLHGAGEQAFTSGADLKALPRDIWLAMPNLSVPCDKPIIAAVSGLAVGAGAT
                     MVMLADMAVADEDARFVYPEARIGAFAGVMAGFPPRMQYKAGLEWLLTGDPMTAQRAY
                     EIGLVNRLAPHGQALEVAMQVASRIAANAPLVVQAMKSIARRTLPASPTELYYPQHRM
                     LQGIAASADLREGVASFKEKRAPRFSGR"
     gene            57939..58916
                     /locus_tag="PKN51_00270"
     CDS             57939..58916
                     /locus_tag="PKN51_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT83558.1"
                     /translation="MTLTRRSLHLLALAALSGAVAPALAQTSKPWPDKPIRFVTPYPP
                     GGSSDVITRFIGDGVSKILGQPVIVENKPGAAATMGTEFASRQAPDGYTFLVAPTAAL
                     ALAPYLMKNVKYTFESFDPVAKIASSYGLVTARKDAPFTDYKGMLAYAKANPGKLTFA
                     TNGVGSIVHMTAVLLHKHAGVQLTHIPYKGAGESVIDLLAGRIDLMYDPATAPRVKSG
                     DLKGLGTTSGIRNPELPNLPTLKEQGFGDGGSFSWFAMFAPKGTPAPVVNKMADAVRQ
                     VLEAPEVKAKLQLSALYPNYEDPATFAKSVRNDAETLRNVIQQEGIKLE"
     gene            complement(59087..>59938)
                     /locus_tag="PKN51_00275"
     CDS             complement(59087..>59938)
                     /locus_tag="PKN51_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNT83559.1"
                     /translation="SYSVHGLAMLPFYIYYSMFGFQRVGDAIWAAADQRARGFLLGAT
                     SGRTTLGGEGLQHQDGTSHLVAATIPNCKAYDPAFAGEMAVIVDAGMREMMVEQKDVF
                     YYVTLMNENYAQPALPADAHPGVLRGCYLFGSYRRTPDGRELPETAPTVTLLGSGAIL
                     TEVILAAELLVRQGIAATVLSVTSWSELARDGLACQQRRLAGADTPGVPWVTQQLSDT
                     QGPVIAATDYVCAVPESVRAFVPEGRSYLTLGTDGFGRSDTRAALRRFFGVDARALVR
                     AARLALG"
     assembly_gap    59939..60038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<60039..61878)
                     /gene="aceE"
                     /locus_tag="PKN51_00280"
     CDS             complement(<60039..61878)
                     /gene="aceE"
                     /locus_tag="PKN51_00280"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNT83560.1"
                     /translation="MHAPIDSQALASSASLPADADPQETAEWRAAFEALIATQGPARA
                     CFMLDELARLARARQLGWRPELNTPALNTIAAQDQPVFPGDLAIEERLGSLIRWNALA
                     MVVRANQAYGELGGHIASYASAADLFEVGFNHFFRAREGLAPGQHRGDLVFFQPHSAP
                     GVYARAFLEGRLSEQDLLHFRQELTAPGAGARGLCSYPHPYLMPDFWQFPTGSMGIGP
                     ISSIYHARFMHYLTDRGLLDCAGRKVWGVFGDGEMDEPESTSALTLAAREGLDNLVWV
                     VNCNLQRLDGPVRGNGRIVDELERLFTGSGWNVIKLLWGSDWDGLLARDLTGTLVRTL
                     GETVDGQMQTFAAKDGRFNRDNFFGQSPELQQLAQGMTDEQIDRLKRGGHDLVKIHAA
                     YAAAHAHRGQPTVILAHTKKGYGMGAAGQGKMTTHSQKKLDEQELIAFRNRFNLPLTD
                     AQAANLEFLKPAVDSAELQYLLQRRRALGGWLPARQTLCEAVAVPALTSYAQFAVAAD
                     GKDMSTTMAFVRMLGNLLKDGQLGPRIVPIVADEARTFGMANLFKQVGIYSSVGQRYA
                     PEDIGSILSYREARDGQILEEGISEAGALASWTAAATSYSVHGLAML"
     gene            complement(62007..63260)
                     /locus_tag="PKN51_00285"
     CDS             complement(62007..63260)
                     /locus_tag="PKN51_00285"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018826 - methionine gamma-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HNT83561.1"
                     /translation="MTRSLDRAGGFSTRAIHFGYDPAEHLGALVPPIHTSATYAFPDT
                     GYGARCFTGEEKGYIYSRIANPTLALLEARLASLEQGAGAVVFASGMGAITATLWSML
                     TPGDEVLADLTLYGCTFSFLHHGIGHFGVTVRHVDMTDPEQVAAALTERTRVVYIESP
                     ANPNMRLADIAAISRLSHARGARVVVDNTYCSPYLQQPLVLGADVSLHSVTKYLGGHG
                     DLMAGAAVFADAELAERVRLYGLKDMTGAVMSAHDAHLVMRGLKTLALRMDRHCQNAQ
                     QVAERIAAHPAAAVVHYPGLPSFAQHALAQRQMRQMGGMIAFELHGGLQAGVRFMDAL
                     RLVTRAVSLGDAETLAQHPASMTHSTYTPEERAAHGIPEGLVRLSIGLEDVEDILADI
                     GQALDHALAPEGAARGGARSLSSVF"
     gene            63394..63873
                     /locus_tag="PKN51_00290"
     CDS             63394..63873
                     /locus_tag="PKN51_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNT83562.1"
                     /translation="MSSPSGSFDHVDRALLEVLQRDGRATVGQLAERVSLSPSPCWRR
                     VRQLEESGVISGYHAHLDRRRIGYGVLGFVQLNLHDHTSSICAAFEREVLALPQVLAC
                     HNLSGRHDYQLEVVAPDLEYFAEFVRSRIRSLPGVREISTSFSLKEIKRTIALPLDH"
     gene            63939..65327
                     /gene="gorA"
                     /locus_tag="PKN51_00295"
     CDS             63939..65327
                     /gene="gorA"
                     /locus_tag="PKN51_00295"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HNT83563.1"
                     /translation="MADFDFDLFVIGGGSGGVRAARFAAQRGARVALAESGRMGGTCV
                     NVGCIPKKLYSYGAHYAEAFEEARGYGWQLPGAPRLDWDALKARRAQEISRLNGIYEG
                     LMTGAGVRRLQGHGRLLDAHTVDVTAPDGGRSRHRARHILLATGGTPVRPELPGHELI
                     VTSDDMFDLPRFPRHLVVVGGGYIGCEMASIFHGLGAQVTLLYRGEQILRGFDDEVRD
                     FTAAELRKAGIDLRVRTEVTRIEAAPDGTRRLTLNDGGVLQADVVLYAVGRRPNVAGL
                     GLEAVGVRMTASGAIAVDEHFATAVPGLYALGDVRGGLELTPVALAEAMALVDHLFGP
                     LPGQARRTMDYEHIPTAVFTHPPIGTVGLTEAQARARVGTIRVYRSDFRPLKHTLSGR
                     DERCLVKLIVDDASDRVLGLHMVGADAGEIVQGFAVALKCGATKAQFDATLGIHPTSA
                     EEFVTLRDVTRT"
     gene            complement(65375..65806)
                     /locus_tag="PKN51_00300"
     CDS             complement(65375..65806)
                     /locus_tag="PKN51_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83564.1"
                     /translation="MKAKLHGVFGLVALLCIVTFWLSTVVAELLLDQASVVFVKNMVV
                     AGMWVLIPAMAATGGSGFSLARSRRGRLVEAKQRRMKMVAVNGLLVLLPSALLLARWA
                     SAGRLDAGFYALQGLELVAGATNITLLALNLRDGRRLAGRR"
     gene            complement(65803..66273)
                     /locus_tag="PKN51_00305"
     CDS             complement(65803..66273)
                     /locus_tag="PKN51_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNT83565.1"
                     /translation="MNALPQIDAISALALAVFRLNGALLHWGDRRVATLGLSSARWQM
                     LGALALADSPLSAPQVGAAMGVSRQGALKQLGVLQGLGLVQVDPNPAHRRSPLYCLTP
                     HGRKCYEQADALWRDQVAQLARQISATQARQATEVLQHIEHVLGSATADLEDAT"
     gene            complement(66331..67680)
                     /locus_tag="PKN51_00310"
     CDS             complement(66331..67680)
                     /locus_tag="PKN51_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT83566.1"
                     /translation="MRVIAGDAWAVFVGQVAVMGFAVVDTIVAGRHSDLSLATLSIGA
                     AVFVTVHVSLMGLFTALLPIWAELRGARRLGEVGGSVRQALYIWAAASLLGLAVLLQP
                     EPLLRWTDVPAGLQPVVVDYLAVLGWSLPAALLFRAFGTLNQALGRPQLVSWLQVLAL
                     AVKLPLSVWLTFGGAGVPALGVVGCAWATFAVHGLLLGLALWLLRTQPLYEPLQLWRR
                     PEPPHGPTLAAFARLGVPAALTMLVEITSFTLMALFVARLGTTATAAHQIAANVAALM
                     YMAPLSLAVASSARVGWWRGAGDEARARAVVMQSFWLAGGLGIALAAMVFVAKPWIAA
                     VYSAQAGVVLLAARLLGWVALLHAADALQTVAMFLLRCYRVTLAPFVVYGVLLWGVGL
                     AGGYLLAYHGVGPLAAQHSPVAFWQAGGGALIVVALLVWLMLGRVSRRAAASVSVTH"
     gene            67871..69034
                     /locus_tag="PKN51_00315"
     CDS             67871..69034
                     /locus_tag="PKN51_00315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNT83567.1"
                     /translation="MTDLSKITCIEDLRVLAKCRVPRMFYDYADSGSWTEGTYRSNQA
                     DFAPIQLRQRVAINMEGRSTATTMIGEPVAMPVALAPTGLTGMQHADGEILAARAARA
                     FGVPFTLSTMSICSIEDVAQHAGPGFWFQLYVMRDRDFIERLIDRAKAAGCGALVITL
                     DLQILGQRHKDIKNGLSTPPKPTLANLINLATKPRWIMGMLGTPRRKFGNIHGHVKGV
                     SDMSNLGAWTADQFDPRLNWGDVEWIKKRWGGKIIIKGIMEPEDARLAVDSGADALIV
                     SNHGGRQLDGAPSAIAALPTIVDAVGTQIEVHMDSGIRSGQDVLKAWALGARGTYIGR
                     AFLWGLGAAGEAGVAKALQIIHKELDLTMAFCGHTRIESVDRRILRPGTFPTP"
     gene            69048..70001
                     /locus_tag="PKN51_00320"
     CDS             69048..70001
                     /locus_tag="PKN51_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT83568.1"
                     /translation="MLHPQAQALIDLLVAHGLPPTHTLTPAEARAFYRERRALTQPAP
                     PDVAELRELRLPGPAGELAARAYRPLGARPEAVLPVLVYFHGGGWVIGDLDTHDTLCR
                     ELANASGCAVVSVDYRLAPEHPFPAAFDDALAATRWVARHAGELGADPTRLAVGGDSA
                     GGNLAAAVALAARDDSDAGGPLPIAFQLLIYPATDMRRGHGSHQRNGQGYILTRETMD
                     YFIGHYLPPGTSPTDWRGSPLLAASHVGLPPALVLTAGFDPLVDEGLDYARALTAGGS
                     RASYVCFGRQIHGFITMGRVLDEANSAVALCATELRRALTP"
     gene            complement(70021..70497)
                     /locus_tag="PKN51_00325"
     CDS             complement(70021..70497)
                     /locus_tag="PKN51_00325"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNT83569.1"
                     /translation="MRRWYEHRIPPPVIDLACAAVMAWLAGAVPAARLWPAGGAWWVW
                     ALALGLALIGALVALAGLLEFRRARTTFNPLAPQRARTVVTRGVYRWTRNPMYLGMLV
                     VLAGWAVWLGSAAALLGLPLSVLLLNRLQIGPEERILRERFGQDYIDYQARVRRWL"
     gene            complement(70543..73530)
                     /locus_tag="PKN51_00330"
     CDS             complement(70543..73530)
                     /locus_tag="PKN51_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNT83570.1"
                     /translation="MFPFSRESNDPQPAPDSPARPAAEAHPLDALTGGVFSAATSGER
                     AQRVRDWLASGPTPEQMQEVFKELSAKDKGAARALRERLDELRRAKGQEAVAAEWAAK
                     AQALLQAARLNIADALAWQRDAAKAGAPLSREPLAGLRTQLAQRVKVVEDLQQRVMVQ
                     REAAILLAQRIELLSTKPWQDAQAQQIGLAADVAQWRQQAAALLADAQWPSVDLRFPP
                     QMEAAQTQLSAVWDAFGAALAQAQAAAADAAAPLPSVPVWADELRAARGEAPAAAAEA
                     DKPARPRIDPEQRAAAHKAVEAGVMLLEQAVTAGHTKNMHSATQALRQSLKTHGRLID
                     DALEARVHAVLVSAGELEGWQRWSADKLREQLVARAEALLVKRRKKAPAQGAPDAAPA
                     PSQAESGQASATEPTAVPAAPEASASPAAPEQTAPEGQPDTIESAAAGADPASAGAEN
                     DAELTARPLPATTLAADEQWVPAMGGRKLQEAIRKLRDEWKAADQGGPPNHALWKRFD
                     RAVSTAHAFVDEWIAGVRAQAAEHKAQREALIDEVRAWAASHADAGEGGDWKAVNRQI
                     HQFAERWRNAGHLSEKQFAELQPRWKEAIHAAALPLENMQKHSTARRQALIQEAEALG
                     AAPQLRIDAVRALQQRWQAEAQAVPLDRKHEQKLWDAFRKPIDEAFNRKSAEREKAQA
                     AMSAHDRAVLEAAKALEAANASGDAQRIRAAMAQLEAATRAQPAPGSEEKPAAAPADG
                     APEAIEIEATEVPTDAPDAPAEGAAPAPAPKPAKPLVAMRGDDRPGARKAEPAPAGRP
                     GERRPAGRDGRAGDRREGAAGRAGERGARRFDDARGPRRSDEARGPRLGDAAFRAQRD
                     AVEQAQAQLRKLAAQAHGEALTQLLGAWQARQPDQVPGVQELGRGVTPALRNAWTQAI
                     GGGAGAPAAEALLRLEMAAEVPTPAEHLQARRALQLQLLTRRNDPSPAQTWGQDTAAV
                     LAGPHDDATARRLQTALKGLLRK"
     gene            complement(73687..77910)
                     /locus_tag="PKN51_00335"
     CDS             complement(73687..77910)
                     /locus_tag="PKN51_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNT83571.1"
                     /translation="MSGSLLSSPPPEPTRAAVAASPAPPERAWWPPRRWRRRLTIALV
                     GLLALWALAWLALPPLLKWQGEKIASAELGRPVHIGAVVVRPWSLQLALHDLRIDAAP
                     GAPPLLSVARLWADAELQSVFRLAPVIDALRIESPALHLTHLGEGRYDIDDLLARLAA
                     RPAAPEPAALPRFALYNIELTDGRIDFDDRAAGRQHQVRQLRLALPFLSSLPAARQIK
                     VEPQLAFVVNGSAFDSRAQALPFDASRQTEASLQLKSFDLQPYLAYLPAALPLRLQAA
                     TLDADLRAAFAQAGAPTLSVSGNLALRGLKAVDAQGAERLAFDALELELDELRPLERR
                     LALRSVELRGPRLALHRRANGQLDLLAQGAPDATDSIAAGAHPAGSEGPSDPASGSAP
                     AGPAGAADGWRLAIGRLALQGGALDFSDASTSAHGAPAARLQLQHLELAARELAWPPD
                     QPLHWEGTAQLTGAPDAGQGAATLAFEGQATRRQAELQARIDGLPLALGAPYLAGVLK
                     PRLDGQLAARLALHWAAPEQAGAPAQLTLSAEQVTLSQLALTDPDAAPAPARGRRRSA
                     LPDDARLVAAEGIELQRLHLDWPARAVRVARLGLRAPLARLAREADGRWMVERWLRQP
                     LPADAPAPTAAAPAWKLTLDELTLADGILGWHDAAVADGPVQAELTQLTLGLRQFELD
                     GRRPMPLQLAARLRAGRSEPGRLSWRGQLTLSPPALQGTADVQRLPLHALQPYFGEWL
                     NVDLLRADASFKGQLELAQTEQGARVRVTGEASLEELRAHSRPGSVVASASASTPAAG
                     DTTPPATPATPVTSAASVTPAAAAQAPRAALAPVAADRAGARSGGLGEELLSWKLLRL
                     GSVDLRLDPGQPAQLGIGETLLSDFFARVVILPDGRLNLQDVLKSEHAAGGSPGAAPA
                     TPPGAMPAAGEFSGTTHSIAAGAPAAGASAPLAAAPAALAPVVRVGPVRLEGGRVDFA
                     DRFIRPNYSADLSELAGTLGAFSSVAPDGQVQMAELNLSGRAEGSAQLRVSGRLNPLA
                     TPLALDIRGQVSELELPPLSPYAVRYAGHGIERGKLSMDVNYRIQPDGQLTASNQLVL
                     KQLQFSEPVAGAPASLPVRLATALLSDSQGVIDLDLPISGSLNDPQFSLGPVIVKAIL
                     NLIGKAITAPFTLLARALGGAGGEDLSQVAFAPGSARLDARARQQLDRVAKALAERPQ
                     LTLTVVGHARLAPEREGWRRQRLQALLAAERRAEATAAPAASAAANPAEDRSPAPDAM
                     PSGAAGADPAGAGAASDATDAMDAEQRLRLLRRLYRRADVPGRPRNAIGLLRDVPQAE
                     MEALLLAHIEVGQDAMRQLAVQRAVAVKDYLAAHGAPADRLFLGAAQAAADAAAAAPA
                     SAASAATSGSAPAAAPAWTPHAELKLATR"
     gene            78663..79484
                     /locus_tag="PKN51_00340"
     CDS             78663..79484
                     /locus_tag="PKN51_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNT83572.1"
                     /translation="MTASTRLAALSSGLRTFAGDVAEGFFEITHNGLALVGVVVVLAV
                     ALLGAHPELRQQGEQQLTEWLTARKVAALGITPELGAIERATAANPRELPREQANLAL
                     WISKKYRVAPEPVAALVAEAYEAGQAAKLDPTLILAVMAIESSFNPFAQSSVGAQGLM
                     QVMTQIHSDKYEHFGGQYAAFDPKTNLRVGVKVLQEYIGRTGSVEMGLKWYVGAANLA
                     SDGGYADKVLAEHARLYQVAKGKPLPAALPGLRAQKPAPASPPEPTAPVEVATLS"
     gene            79679..80929
                     /gene="glyA"
                     /locus_tag="PKN51_00345"
     CDS             79679..80929
                     /gene="glyA"
                     /locus_tag="PKN51_00345"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNT83573.1"
                     /translation="MFNRSSHTIAHTDPELWAAICAEHQRQEDHIELIASENYASPAV
                     LAAAGTQLTNKYAEGYPGRRYYGGCEHVDVAEQLAIDRVKQLFGAEAANVQPHCGASA
                     NIAAFMAFLKPGDTIMGMSLAEGGHLTHGMPLNMSGKWFNVVSYGLNAQEAIDYDAME
                     KKAHETRPRLIIAGASAYSLEIDFARFARVAKDVGAIFMVDMAHYAGLIAGGCYPNPV
                     PHADVVTSTTHKSLRGPRSGFILMKAEHEKAINSAVFPGLQGGPLMHIIAAKAVAFKE
                     ALQPEFKQYAAQVRKNAAVVAQTLTERGLRIVSGGTQSHVMLVDLRAKGITGKEAEAV
                     LGSAHMTINKNAIPNDPEKPMVTSGVRIGTPAMTTRGFKEDEARATAHLVADVLDQPR
                     DEAHIAAVRAKVAELTKRFPVYGN"
     gene            81063..81512
                     /gene="nrdR"
                     /locus_tag="PKN51_00350"
     CDS             81063..81512
                     /gene="nrdR"
                     /locus_tag="PKN51_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNT83574.1"
                     /translation="MRCPFCAHTETQVVDSRVSDEGDFIRRRRQCAHCGKRFTTYERP
                     DVSYPSIIKKDGRRVPYARAKLQGSLQLALRKRPVSTEQQDAAIERIEEKLLATGARE
                     VSSSRIGELVMRELKKLDKVAYVRFASVYRSFEDIDEFRALVDEVKR"
     assembly_gap    81656..81755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(81764..82210)
                     /gene="trxC"
                     /locus_tag="PKN51_00355"
     CDS             complement(81764..82210)
                     /gene="trxC"
                     /locus_tag="PKN51_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNT83575.1"
                     /translation="MTDTLHVVCPHCHTTNRVRAGQLGSAPTCGQCKQPLFTGQPLAL
                     DDAAAFDRHLARSQIPLLVDFWAPWCGPCRMMAPAYAQAAALLEPQFRVAKVDTEAAP
                     ALGTRYNIRSIPTLALFVHGREVARQPGALTSPAQIAQWARAQLAR"
     gene            complement(82207..82548)
                     /locus_tag="PKN51_00360"
     CDS             complement(82207..82548)
                     /locus_tag="PKN51_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83576.1"
                     /translation="MKSLLSPPRTAIVLARLLERLDASRQPVDAHQYQGVAARLAALL
                     SDPEVDWQPLLADSPAAAALYENLHYGEAGLCRTPLGAAMRAELQARQAIAAARAAAA
                     PGTPSDAEDRP"
     gene            82709..83203
                     /locus_tag="PKN51_00365"
     CDS             82709..83203
                     /locus_tag="PKN51_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNT83577.1"
                     /translation="MATFTLSSPDIPSGGSVPRHFEFDGFGCSGDNRSPVLRWSGAPA
                     GTRSFAVNVYDPDAPTGSGFWHWYVIDLPASVSELPANAGALGGALLPAGARQIRNDY
                     GAYAWGGVCPPPGDPPHRYVFTVHALSVERIDVPDDAPAALTGFMVNAHTLAKASFTA
                     TYGR"
     gene            complement(83240..83713)
                     /locus_tag="PKN51_00370"
     CDS             complement(83240..83713)
                     /locus_tag="PKN51_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNT83578.1"
                     /translation="MRLTQWTDYALRVLMYCAACHGRAQPVTISEVAEKHGISRSHLT
                     KIVQQLAALGLLDTTRGRGGGMRLMRPAEQINLGAVVRATEGDFELVECFNADLNTCR
                     LSSHCHLKNVLGRAMQGYLAVLDGVTLADLVPPAGAPQGRSAQPMRFVPGLPAPG"
     gene            83801..84523
                     /gene="ytfE"
                     /locus_tag="PKN51_00375"
     CDS             83801..84523
                     /gene="ytfE"
                     /locus_tag="PKN51_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HNT83579.1"
                     /translation="MNATTQATRPALDADQAIGQIAVELPGATAVFRRLKLDFCCGGQ
                     LSLRQASADKGLDLQAVMNVLSTLQRPSQLPDTGTPGELIDHILTRFHDVHRQQLPEL
                     IRMAHRVEAVHRGNPDVPAGLGDALEAMQQELLTHMHKEEAILFPMLRQGGNSFVSQP
                     IGVMRHEHNDHGAALEHLASLTHDMTPPMGACNTWRALYTGLAQLRDDLIQHIHLENN
                     VLFPPFEAATAGGCGSSGCGCS"
     gene            complement(84558..84776)
                     /locus_tag="PKN51_00380"
     CDS             complement(84558..84776)
                     /locus_tag="PKN51_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNT83580.1"
                     /translation="MADTARVDDRLDALEVKAAFAEDLLEQLNLTVYRQQQQIERLQR
                     ELTQLRQQLSEGSGAAPRSLRDELPPHY"
     gene            complement(84782..85984)
                     /locus_tag="PKN51_00385"
     CDS             complement(84782..85984)
                     /locus_tag="PKN51_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNT83581.1"
                     /translation="MSLPFAQRLEHVETSAIRELFKLLGKPGIISFAGGFPDSAMFDV
                     AGIGEAVNAALATEPGAALQYGATEGHPPLREQLAAFMRSKGVSELTPEQLIVTTGSQ
                     QALDLLGKCLVDPGSKAIVEGPTFLATIQCFRLYGADLVSAPIDGEGVQVDELERLIR
                     THRPKFVYLIPTFGNPSGAMLSLARRRRVLELAVQYQTLIIEDDPYGDLYFDAPPPPS
                     LLALSSQVPGSRDWLVHCGSLSKVLSPGLRVGWMVAPPALLAKATMCKQFSDAHTSTF
                     AQATAAQYLAAGRMPGTLARVRQVYGARARAMGDALRQELGAALEFVQPQGGLFVWAR
                     LTGAGGALRDGNELARRAIERGVAFVPGAPFYCAQPDVSTLRLSFATVGEERIREGVA
                     RLAQALAG"
     gene            86047..86685
                     /gene="nth"
                     /locus_tag="PKN51_00390"
     CDS             86047..86685
                     /gene="nth"
                     /locus_tag="PKN51_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNT83582.1"
                     /translation="MTPAQIDRFFTTLQAANPLPASELEYTSVFELLTAVLLSAQATD
                     VGVNKATRRLFPVANTPQQVLALGLERLESHIRTIGLYRSKARHLMQTCAILVERHGG
                     EVPRTREALERLPGVGRKTANVVLNVAFGEPTMAVDTHIFRVGNRTGLAPGKTPLAVE
                     QQLLRRVPERFLPHAHHWLILHGRYVCLARKPRCWECAVADCCDFQPKTPAP"
     gene            complement(86744..87958)
                     /locus_tag="PKN51_00395"
     CDS             complement(86744..87958)
                     /locus_tag="PKN51_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNT83583.1"
                     /translation="MTRPLDGITVISLEHAVAAPFATRQLADLGARVIKVERPGGGDF
                     ARGYDRRVRGQSSHFTWLNRNKESLALDVKQTEAKAALLQLLKTADVLVQNLAPGAAA
                     RMGLSYEALKGHNPRLIVCDISGYGANGPYRDKKAYDLLIQSEAGFLSVTGTPETPSK
                     SGISVADIAAGMYAYTNILSALLLRGKTGEGSHIDVSMLEAMGEWMGYPMYYAFDGAP
                     PPPRTGASHASIYPYGPFAAGDGGTVMLGLQNEREWQAFCEQVLRRPEVAVDARFCSN
                     AARNQHRDELQALILQEFATLTAAQVVERLDAAGIANARVNDMAGLWAHPQLAARERW
                     REVDTPAGRIPALLPPGVSSAFEYRMEQVPAVGQHNAAILAELGWTAERIAALAAAPA
                     AQETIKTIAAGA"
BASE COUNT        13839 a        31588 c        29527 g        12705 t
ORIGIN      
        1 ggcagcgcct ggccgacgtc ctggcgcacg aaatgcgccg gcacgcgcgc caccggcaga
       61 ccgtccaggt acacgcgcac ccagggcgtg gcgccggccg gcagcgcggc gtcgtgcgcc
      121 cagccgctga tggccagcgt gccggcggcg gcatcgtcca ggtgctgcca gaagtgctgc
      181 agcggctcgc ggcggatgtc gcgcgaaggc ggatcgacgg cgtcgaagct ggcgaagtcc
      241 gaccccaggt aggcgttgag cgccgccacg tggccgaagc gctggcgcag aaaggcgcga
      301 aagccgcggc gcgaggcgtc ggcgtagtcg gtcagcacgt agggccggtc gtggcccatg
      361 ccggcctcga agtcggggta caggtggtgc acctcgccca gcaggttcag gccgacgatg
      421 cgctggcgcg ccgccgccgg cagcgcgcac acgccctgca ccagcgcgcc gatggcctgc
      481 tcgcgccgct gggtgatggc gttgtcggtg cgggcgatgc tccaggggta cagcggccag
      541 cccatgaact ggtccaccgg cagcgggccc tgcggcgtgt gcgccaggtt ggccgggtcc
      601 tgcgccagca ccggctcgat cggcgcctgc tcggaaaaat gggtggaaaa caggtacagc
      661 accaccggcc gctgcacgtc gcgcacggtg cggacgatgc gctgcaccgc ctgctggtcg
      721 atggcccagc cggcgccctg cggctcgaac aggttgagca gcggcaccac cagggtgtag
      781 ccgagctcga agtgcccgtc ggcactgcgg cgcgggccca gcgggccgag cgtggcctcg
      841 atgcgctgcg cggccgagcc gcgctcgccg ctgcaggcgg gctccagcgg cgtctgcgcc
      901 ggcgccagtt ccagacagtc ggccagctcg ccgatggccg gtgccagcag cagcgcgcgc
      961 tgcccgccct gccacagcca gaccgacagg cccagcagcg cggccagcag cgcggcggcc
     1021 agcagcgcgc tcaccgcgtc gacggcgcgc gcgctcatcg gccgcacccg cacgagcacg
     1081 cctcgggctg ggcggcagca caccgaccag cctggcgcgc ggccgggtcg gccggcatca
     1141 ccgttgggca gaaaactatc gaattcatag ctgctcgcgc agattcagca agcggcgcag
     1201 gccaatgggt tgcccatcgg atgcttgccg gacgggcctc aggccttcac cagctcgcgg
     1261 aaagtctggc gaaacttggc caccttgggc gccgccgtgg ccacgcagta gccctgggtc
     1321 gggttgcgct cgaaaaagtc ctgatggtag tcctcggccg gaaagtaatg ggccagtggc
     1381 gccacctcgg tcacgatggc gccgtcgtac agcccgtcct ggctcatctc gcgaatcagc
     1441 gcgtgcgcca cgtcggcctg ctcggcggtg gtgaagtaca cgccgctgcg gtactgggtg
     1501 cccacatcgt ggccctggcg gttgagctgg gtcgggtcgt gcaccagaaa gaagatctcc
     1561 aggatctggc gcgtgccgat ctgccccggg tcgtacacca gcttgaccac ctcggcgcaa
     1621 ccggtggcgc cggtgcacac ctgctcgtag ctgggcgccg tggcctggcc attgctgtag
     1681 ccgctttcga cgtcgagcac gccgcgcacc tgcttgaaca ccgcctcggt gcaccagaag
     1741 cagccgccgg ccagcaccag ggtttgactg tcagacatcc atccactcct tcgatcgggc
     1801 cgcaccaggc cccgtgtcgc ccacgagcaa ccaccgcccg cacccggccc cggcgtgcat
     1861 tgtgctccgc cgccggccag cgccagcccg cacccatgcg acgcgccctg gcggcagacc
     1921 ggcgaccccg ccggcggcgc ccgacgccca agcccggcgc ttcgtgattt agtaatcaat
     1981 aatgtctaaa tatttccggc aacggcgttt caggaccggc ccagtccgct cagaatcagg
     2041 ccgcaccggc agcacctcat cactcgccga acgcatcgat acccgcagac catgaacgcc
     2101 atgccacacc cccccacccc gcggcgctgg cgcgccgctc ggcttgtacc cgccctctgc
     2161 gctggcctgc tggccggcct ggcgccaccg gcgcaggcgg cctgggaacc gacggcaccg
     2221 gtggagctgg tggtgcccgc cggcaccggc ggcggtgccg accagatggc ccgcttcatc
     2281 cagcagatgg tgagcaagca tggcctgatg aagcagccgc tgaacgtggt caacaagacg
     2341 ggcaattcgg gggtcgaggg cctgatggac gtcaaagcgg cgcagggcaa cccgcacaag
     2401 ctcatcatca cgctgtccaa cctgttcacc gcgccgctgg ccaccggcac gccgtttgcc
     2461 tggtcggaca tcaccccggt gcagatgctg gcgctcgacg agttcgtgct gtgggtagcc
     2521 aagggctcgc cgtacaagtc gcccaaggac gtgctggatg cgctgcgcgc cagcccggcc
     2581 ggcagcctca ggctcggcgg caccggcacc aagcaggaag accagctgat cagcgtgctg
     2641 ctggaaaccg cggccagcgc gcgcatccag tacgtgccgt tcaagggcgg cggcgacgtg
     2701 gcacgggcac tggccgccgg tgaggtggac ctgacggtca acaacccgat cgaggccgaa
     2761 aagctgtggc gcgaaggcaa gctgcggccg ctgtgcgtgt tcgacggcag caaactggcc
     2821 tatcccgaca aggtggccga caaccagagc tggagcgacc tgcccacctg catgtcgttc
     2881 ggcatcccgg tggaatacct gatgatgcgc ggcatcttca tggcgccggg tgccacgccc
     2941 gagcaggtgg cgtactacgc gcagctgctc gacaaggtgc gcgcactgcc cgaatggaac
     3001 gctctgatga agcagggcgc ctacaagcag accaccatgg ccgggccgag cttcagcagc
     3061 tggctcgatc gcagcaaccg ctttcaccgc accttgatgc gcgaagccag gttgacggcc
     3121 gccgcccccg gcgccgccag gaactgaggc attgccggtc acgccggcgc ggcggccagc
     3181 aggcgctcga tcgcctgcca ctgacccggc gtcaccggtg tgatcgacag ccggttgccg
     3241 cgccgcagca ccagcatgtc ggccagcgcc ggcacgccgc gcagctcggc caggcccagc
     3301 aggcgcgtct tgcgcagcgc ctgcacgtcc accagcagcc agcgcgggct gtcgggccgg
     3361 gcggtggggt cgaagtacgc ggagccgggg tcgaactggg tcgggtcggc atgggcggcg
     3421 gaggccacgc gcgcaatgcc ggcgatgccc ggctcggcac agctggagtg gtagaacagc
     3481 acgccgtcgc ccacccgcat ggcgtcgcgc atgaagttgc gcgcctggtg gttgcgcacc
     3541 ccggtccacg gcacggtctg gccaggcgcc gccagcgcgt cgtcgatcga gcactcgtcg
     3601 ggttcggatt tcatcaacca gtactggcgc ggcgtcgtca tggcgccgat tgtgccgccg
     3661 acaatgctgg gcatgaccga ccacgcccac cccttcgcca ccctcacccc cgacttcgtg
     3721 ctggacgcgc tggccgccct ggggctgatg ggcgatgggc gactgatgac gctcaactcg
     3781 tacgagaacc gcgtctacct ggcgcatctg gagccgggca cgccgctggc cgaggcgcac
     3841 ccggcggtgg tgctcaagtt ctaccgcccc gggcgctgga cgcaggcgca gatcgaggaa
     3901 gagcacgcct tcgcgcggga gctggccgcg gccgaggtgc cggtggtggc gcccctggtg
     3961 atcgacgggc gcacgctgca ccggcacggc gacttctggt tcagcctgac gccacggcgc
     4021 ggcggccgcg cgcccgagct gggcgacgcc gacacgctgg catggatggg ccgctttctg
     4081 gcccggctgc acctggtggg ggcgcgccag gcgtttgtgc accgccccgc gctcgacctg
     4141 cgcagccacg gcgtggagcc gcgtgaatgg ctgctggcgc acgctgcgct gccgccggag
     4201 gccgagcgcg catgggccga agtcagccag gatgctatcg atcacatagc tgctggcgca
     4261 gattgggcaa gctctgaaac ccattttgac catccaaccc ggccgatcgc cacgctgcgt
     4321 ctgcacgccg actgccatgt cggcaacatc ctgtggacgc cgaccgagca gcccggcggc
     4381 ggcccgcact tcgtcgatct ggacgactgc cgcagtggcc cggcggtgca ggacctgtgg
     4441 atgctgctgg ccggcgagcg cgccgagcgc cagcgccagc tgggcgcgct gctggacggc
     4501 tacgagcagg tgcgcgcgtt cgaccggcgc gagctggcgc tgatcgagcc gctgcgcacg
     4561 ctgcgactga tccactacag cgcctggctg gcgcagcgcg cgcacgaccc ggcctttgcc
     4621 gcagcctttg ccggcttcgg cacgcccgac tactggcgcg gccagacggc gctgctgcgc
     4681 gagcagatcg aggccatggc cgagccgccg ctgtgcgcct gagcgcctgc gcgcgccgcc
     4741 gcgccagcgg acaacggcgc ggccccgccc gctcgatctt gacgccgttc aggcgcctgc
     4801 gccaaaatac tgacccccca gtcattaaat gaacaccctc caggacctcc cgtgcccgcc
     4861 gtcccggcgc acgcgccgca agcaggcgcg gccgggcgag ctgctggacg ccgcgctggc
     4921 gctgttcgtc gaaaagggct ttgccgccac ccgggtggaa gaagtcgcca cccgcgccgg
     4981 cgtctccaag ggcacgctgt ttctgtactt tcccagcaag caggcgctgt tcaaggccgt
     5041 ggtgcgcgaa aacgtcggcc gccacctgga ccaggcgctg gccgatctgg ccagctacac
     5101 cggctccagc gccgatctgc tgcgcgaatt cctgcgccgc tggtggaccc attacggcaa
     5161 cacgcccgcc gccgggctgt gcaagctgat cgtggtcgag gtggcccact ttcccgaact
     5221 ggcgcgcgac taccaggagc aggtggtgct gcccagccat gagctgctgc gccgcgtgct
     5281 ggagcgcggc atcgccagtg gcgaattccg ccgcgtcgat gtggcgcaga cggtgccgct
     5341 catcatcgcc cccctgctgc agctggtgct gtggcgccag tggccgaccg actgcatgcc
     5401 ggcgccaacc tccgatcccc aggccctgct ggcgcagcac gccgacctgc tgctgcacgg
     5461 cttgcgcccc gacaccgcaa caccatgaat ccgtccttct cccgtgggct cgtccgggcc
     5521 ttgttgctgc tggccgtgct ggccgccgtg ctgctgggct ggcgcggcta ccaggcccgg
     5581 caggccaagg ccccggcgcc ggctgcggcc gcggccagcg cgccggtgat cgaactggcc
     5641 gcgaccgacc tggcgcacgc acgccaggtg gcgctgggca cggacatccc catcaccggc
     5701 acgctcaagg cggtgcgctc ggccctggtc aaggcgcgcg tgccgggcga gctgcaaggg
     5761 ctgacggtgc gcgaaggcga ccgcgtgcgc gccggccagg tgctggcccg catcgagccg
     5821 accgagtaca gcgagcgcct gcgccagacc cagcagcagg ccgacgccgc gcgcgcccag
     5881 gtggacatcg cccagcgcca gtacgacaac aacgccgcgc tggtcaacca gggcttcatc
     5941 tcgcgcaccg cgctggacaa ctcgctggcc agcctggaag cggcgcgcgc caaccaccgc
     6001 gcggcgcagg cgggcgtgga agtggtgcgc aagtcgctgg ccgacaccgt gctgcgcgcg
     6061 ccgatcaacg gccagatcgc gcagcgcctg gcccagccgg gcgaacgcgt ggcgaccgag
     6121 gcgcgcatcg tcgaaatcgt cgacgccagc gagctggagc tggaggccgc gctcagcccg
     6181 gccgattcgg tggcggtgcg ggtgggccag agcgcgcacc tggacatcga gggcgtgacc
     6241 cagccggtgg ccgcgcgcgt ggtgcgcatc aaccccagcg cccaaccggg cagccgcagc
     6301 gtgctggtgt acctgagcgt ggcgccccag cccggcctgc gccagggcct gttcgcccag
     6361 ggccagctgg ccaccggcga gcagcaggcg ctggccgttc ccctgagcgc cgtgcgcacc
     6421 gacaaaccgg cgccctacgt gcaggtggtg gcgggcgagc gcatcgccca ccggccggtg
     6481 cgcctgggcg tacgcggccg cgtggacggc gagccgtggg tgcaggtcga aggcgtggac
     6541 gccggcgcca ccgtgctgcg cggcagcgcc ggcgtgctgc gcgaaggcac ggccacccgt
     6601 gtgccgacgc cgcccgcccc ggccagcagc gcccccgccg aggccgcgcg ctcgtgatga
     6661 cccgcgcccg catgcccgcc agcgccgccc gggtggcacc acccagcggc cagatagcta
     6721 cctttttgat agctgctggc gcgcattccg caagcgccaa aggcattttc tacatgtatt
     6781 catcgggccg ccgcgcctga gcctcgcacc ccgccatgtg gttcaccaaa gtcagcctca
     6841 acaacccggt gttcgccgcc atggtgatgc tggccttcgt ggtgctgggc ctgttttcgc
     6901 tgcagcggct gcaggtcgat cagttcccca acgtcgactt cccggtggtg gtcatcacca
     6961 ccgaataccc gggtgcggcg cccaccatcg tcgagagcga agtcaccaag aaggtcgagg
     7021 aagcggtcaa ctcggtggcc ggcatcaacg ccctgtactc gcgcagctac gagaacctgt
     7081 cggtggtgat cgtggagttc cagctgcaca tcgagggccg caaggcggcc gaggacgtgc
     7141 gcgaaaagat cggccaggtg cgcccgctgc tgcgcgaaga ggtcaaggag ccgcgcgtgc
     7201 tgcgtttcga cccggccagc cgcgccatct ggtcggtggc ggtgctgccg caggccagcg
     7261 agggtcgggc cgcgccctcg cccatcgagc tgaccaactg ggccgatcag gtgctgaaga
     7321 agcgcctgga aaacgtgcgc ggcgtcggtg ccgtcaacct ggtgggcggt gccaagcgtg
     7381 aaatccacat ccagctgcga ccggccgcgc tggaagccct gggcgtgacg cccgagcagg
     7441 tgatggccgc cgtgcgcggc gacaaccagg accagccgct gggcgcgctg cgcacccccg
     7501 cgcaggagct gacggtgcag ctgcagggcc gggtggagcg gcccgaggac ttcggccgcc
     7561 tgatcgtggc gcggcgcggc ggcgcgccga tccacctggc gcaggtggcc gacgtgcgcg
     7621 acggcgcgca ggagcaggaa tcgctggcgc tgcacaacgg cgagcgcacg ctgctgctga
     7681 acgtgcagaa ggcgcaggac gaaaacacca tcggcgtggt cgacgggctg taccgcgcgg
     7741 tggccgagat ccgcgccgag ctgccgcccg gcgtgcggct ggagccggtg atcgaccagt
     7801 cgcgcccgat ccgcgtggcg gtgcagaacg tgcgccagac gctgatcgag ggcgccgtgc
     7861 tgacggtgct gatcgtgttc ctgttcctca actcctggcg cagcaccgtc atcaccggtc
     7921 tgacgctgcc gatctccatc atcggcactt tcttcttcat gcaggtgctg ggcttcaccg
     7981 tcaacatgat cacgctgatg gcgctgtcgc tgtgcgtggg cctgctgatc gacgacgcca
     8041 tcgtggtgcg cgaaaacatc gtgcgccacg cccagatggg caagcgctcg tacgacgccg
     8101 ccatggacgg cacgcaggaa atcggcctgg cggtgctggc caccacgctg tcgatcgtgg
     8161 cggtgttcct gccgatcggc ttcatgcagg gcatcatcgg caagttcttc cacgagttcg
     8221 gcctgaccat cgtggcggcg gtgctgatct cgatgttcgt cagcttcacg ctcgacccca
     8281 tgctgtcgtc ggtctggcac gaccccagca tccatgccca cgggcagcgc agcggaccgc
     8341 cggtgacgct gtacgaacgc accatcggcc gcgtcaccgc ctggttcgac cacgccagcg
     8401 accgcctggc cgacggctac cagcgcctgc tgggctggtc gctggcgcac aagccgctca
     8461 cgctgctgat cgccgccgcc atcttcggcc tcagcgtggc catggtgcgg ctgctgggca
     8521 ccgaattcgt gcccaaggcc gacttctccg aaaccaacgt cgccttctac acccctcagg
     8581 gttcgtcgct cgaggccacc gaggccaagg cgcgccaggt cgacgccctg ctgcgcagct
     8641 accccgaggt gcgctacacc gtcgccaccc tgaacgccgg caacgcccag ggcaagacca
     8701 acgccagcct gtacatccgc ctggtcgacc ggcacgaacg cacgcgcaac gccgacgatc
     8761 tggcggccga cttccgccag cgcctgcgcg ccgtgcccgg catcaccgtc acccaggccg
     8821 gcctgctgga gccggtgggc gggcaaaagc agatcgagtt ctcgctgcag ggcccggacc
     8881 aggccgagct cgaacgcctg gcgcgcccgc tgatggagcg cctgcgcgac atccccggcc
     8941 tggtcgacct ggactccagc agcaagcccg acaagccgac ggtggaagtg acggtcaagc
     9001 gcgcggccgc ctccgagctg ggcctgtcca gcgcgcagat cgcggcgccg ctgcgcaccc
     9061 tgatcgccgg caccacggtg ggcaactggc gcgcgcccga cgaccagacc tacgacgtgg
     9121 tggtgcggct ggcggccgac gcccgcagcg tgccgcagga cctggagcgc ctgccgctga
     9181 ccgccggcct caacgccgac ggcacgccgc gcatcgtgcg cctgaaccag gtggcgacgc
     9241 tcaacgaagc cagcggcacc aaccagatca accgtcgcgc catgctgcgc gaaatccaga
     9301 tcaccgccaa cacgcacggc cgcgccaccg gcgaggtgtc aggcgacatc cgcgccgcgc
     9361 tggcgcagat gccctttccg cccggctaca gctactcgtt cggcggcgcc accaagaaca
     9421 tggaggagtc cttcacctac gcgctgcagg cgctggccat ggccatcatc ttcatctaca
     9481 tgatcctggc cagccagttc aaaagcttcg tgcagccgct ggcgctgatg acctcactgc
     9541 cgctgacgct gatcggcgtg gtgctggcgc tgctgatgtt cggctcggcg atgtcgatgt
     9601 tttccatcat cggcatcgtg atgctgatgg gcctggtgac caagaacgcc atcctgctgg
     9661 tggacttcgc catccgcgcg cgcgaaggcc gggtcagcga aggcggcgtc accgagccgc
     9721 tgccgcgcga gcaggcgctg ctggcggccg cgcgcgtgcg gctgcgcccg atcctgatga
     9781 ccacgctggc gatggtgttc ggcatggtgc cactggcgtt tgccgtcagc gaaggctccg
     9841 agcagcgcgc gcccatgggc caggcggtga tcggcggcgt catcacgtcc tcgctgctga
     9901 cgctggtggt ggtgccggtg gtgtactgct acctggacga cctggccggc tggctgaagc
     9961 ggcgcctgca ccgcgcgccg cccgatgcgt ccgcgggctg accgataatt catggtttac
    10021 ccgacctttc tgccctggag cctttgagat gaacgtcgaa caagcccgct tcaacatgat
    10081 cgagcagcag atccgtccct gggacgtgct ggacgcccac gtgctggatc tgctggcggc
    10141 ggtgcggcgc gaggacttcg tgccgccggc gcaccgcgcg ctggcgttcg ccgacctcga
    10201 aatcccgctc aaaccaggcg agcaggccgt ggcccgcggt caggtcatgc tggcgccgcg
    10261 cgtggaagcg cgcatgctgc aggacctgca ggtggccaag cacgaaaagg tgctggagat
    10321 cggcaccggc tcgggtttca tggccgccct gctgggtcac cgcgcgcagc gcgtgctgag
    10381 cctggagatc gacgacgagc tggcccgcac cgcgcacgcc aacctgcagc gcgccggcgt
    10441 gctgaacgtg gaggtgcgcg cgctcgacgg cgcccgcacc gacctgtcgg cgcacggccc
    10501 gttcgacgtc atcgtgctgt ccggttcggt ggccgagctg cccgacgccc tgctggcgct
    10561 gctcaagccc ggcggacggc tgatggccat cgtgggccac gagccggtga tgcgcgccac
    10621 cctggtgctg cgcaccgaca acggcctcgt cagcagccag ccctgggaca gcaacgcgcc
    10681 gcgcctgtgc cacttcccgg agccttcgcg cttccaattc tgaagcgcct ggcgcaagca
    10741 ggacaagcgc tgcacgatga ttgaacaagt atctccgcgc gatctggcaa gctggctgcg
    10801 ggccgccggc ggcgaccgcc agccgctgct gctggacgtg cgcgaacccc aggaatggcg
    10861 cctggccagc gtccagatcg acggcgccga actgcagcag atggccatgc acaccattgc
    10921 gccacgcctg cacgaactcg accccgcgcg cccggtggcg gtgctgtgcc accacggcgg
    10981 gcgcagcatg caggtggcca tgttcctggc gcagcgcggc tttgcccgcg tggccaacgt
    11041 ggcgggcggc atcgacgcct gggccttgca actcgacccc gccctgcccc gctactgaag
    11101 gaccgccgat gccgctgccg tttcgccgcc cccgggcccg cctggccctg ctgctgctct
    11161 ggggtgccac gaccgcgcag gcgcagtccc tgctgcagct gtaccagacc gcgcacggct
    11221 acgacgccac ctaccgctcg gcgctggcca acgcccaggc cagtcaggcg cgcgccgacc
    11281 aggcccgcgc cggcctgctg ccgcagctgg gcatgcaagc cggagcgcag cacaactggg
    11341 ccgacaccca tgtgctgggc ggcggcagca gccgcagctt caacaccctg aacgccgccc
    11401 tggtcggctc ccagcccctg tatcggccgg ccaaccgcat cgcctgggac cagggccagc
    11461 gcgccgccga ggccgcccag atcaatctgg tcggtgccga gcaggacctg atcgtgcggc
    11521 tggcgcaggc ctatttcgac gtgctggcgg cgcaggacgc gctggcgtcg gtgcgcgctc
    11581 tgaaggccgc cgtgaacgag caattgcagg ccgcgcggcg caacttcgag gtcggcaccg
    11641 ccaccatcac cgacacccgc gaggcgcagg cgcgcttcga cctcgccacg gcgcaggaga
    11701 tcgctaccga aaacgatctg cgcgtcagaa aactcgcgct cgaccagctg gtgggccgca
    11761 gcggcagcga accgcggccg ctggcacagc ccatcacgct gccggcgccg cagccggacg
    11821 acgtcaccgc ctgggttgaa cgggccgccg agcagcaccc cggcgtgcgt caggcccgcg
    11881 cggcgctgga catcgcgcgc ctggaaaccg acaaggcccg caccggccac cggcccacgg
    11941 tggacctgca ggccagcgtc ggccagaccc gctaccccga tggcaacccc agcctgtcgg
    12001 cggcgccgcg cgccagcgcg cgcaccacca acgccagcat cggcgtggtg ctgaactggc
    12061 cgttgtttgc cgggttcgcg gtggaaaacc gcatccgcga gaccctgtcg ctggaagacc
    12121 gcgcccaggc cgatctggac cagctgcagc gcagcgtgtc acaggccacg cgggcggcgt
    12181 ttttcggtgt gcaatcgggc ctcgggcagg tcaaggcgct gcaggcggcc gaagcctcca
    12241 gccagaccgc gctgcaggcc aaccagctgg gctaccaggt cggggtgcgc atcaacatcg
    12301 acgtgctgaa cgcgcaaagc cagctctatc agacgcagcg cgacctggcg cgcgcgcgct
    12361 acgacgtgct ggtgggcacg ctgcgcctga agcaggccgc cggcacgctg tcgcaggacg
    12421 acctgatccc gatcaaccag ctgctggtgc cctgatgcgg cgccgcggtg agcgccgaca
    12481 ccgcggtcat cgacgcgctg cgcggccagg cctgaaggcc ggccagcggg tgggcgcgct
    12541 gcgccaccgg ccgataccaa cttgcctgca caatgaccca tgacggatgg cctcgctgcg
    12601 cttcgatgac ccatgggtca gcggtcatgc cggcgcagcc ggtacttggg tcacagttct
    12661 gatcgcgcaa ccgtatccga gccaaaagtg gccacgacgc ttgcctgtcg tgcgccataa
    12721 gctatcaaaa agatagccat tcactcgacc gaccgggtcg gtgcctgggc ccgcgggtcg
    12781 ggtttggctt ccagcgcggc ctccagccac agtgcggcca cgaagcgctt gttgaacagc
    12841 agccacagca ggatgggggc caccggcgtc agcatcagaa agccgaactg gtaaccgtag
    12901 gccacggcgt tctgggccag tggcgaccat ttcagatacg ccgcgccgta cgtgccggac
    12961 atcagcacca cgtcgcgcag ccactgaaag cagatgcccc aggcctgggc cggaatcagc
    13021 agcagactgc cgatcagccc cttgagccac cagcgctcgg tgcgcgaagc cagcagcatg
    13081 gcccacagca gcaccaggcc atagccatag gtcgggtagc tggcctccgg tgccagctcg
    13141 cccagcacat cgccctgcgc gcccggcatg cgcacctgca ccagggtgtg cagcaccgcg
    13201 ctgacgcctt cctgcgccca gccgtcgacc cacggaaaca gcgcctgcat caccgtgccg
    13261 gccagccaga tcaccggcat cgccagccag cgcgcggccc agtaccacag cggcagcagc
    13321 agggccagcc acaggatcga cagcaacaga aagcgaccga tcagcgtgtt gcgcatggcc
    13381 aggctcccgt cacgacgccg cggccgatgc gccggccgcc ggttgcgggt cgcgcttggc
    13441 gacgtagcgc atccacagca gccacaccac cagcacgtcc agcatgatca gggcctgcca
    13501 catgtacagg tgggcgaact cgaacacctt gaagttccac tgccccagga agaacaggct
    13561 gacgatgcgc agcaggttga ccgcctgcac ggcgatgaag cccaggccga tgccccaggc
    13621 cttcatggcc cagctggaag gaaaggccag catcgccgcc accagcacga tggtggcctc
    13681 gacgccattg cagccggcct cgatcgacac cccgaagccg gtcttggcgc tttgcagcac
    13741 gcggccatac gaaatcaccg tcgggtcgat caggtgcacg atggcggcac tgaccttggc
    13801 cagccagccg gtccacggat gcaccacggc cgtctgcacc ggattgagca tctcgatgcc
    13861 gaacaacacc gcctgcacgg tcaggaaaat cagaaaaaag cgcttcacgg tacggcccga
    13921 atccttgaca actccagtgc gccgtggcac ctgatgcgcc actgccctgg ctcacagtgt
    13981 agcggcttgt atcctcgggc tagtggaggg tccatgcggg gtccacgggg cgcccggtcg
    14041 ataatggccc ctgccgcttc tttgcccgcc acccaccgag cgggcaccca ccttcgaaat
    14101 atttcaggtg ttttcagcct ctggtgcacg actggcgtgc gccatcagct atcaattcaa
    14161 gagtaatcta tggcccagta cgttttttcc atgaaccgcg tcggcaagat cgtgccgccc
    14221 aagcgacaca tcttgaagga catctcgctg tcgttttttc ccggcgccaa gatcggcgtg
    14281 ctgggcctca acggctcggg caagtccacc ttgctgaaga tcatggccgg catcgacaag
    14341 gacatcgagg gcgaagccgt gccgatgccc ggcctggaca tcggctacct gccgcaggag
    14401 ccgcagctcg accccgcgca caccgtgcgc caggaggtcg aactggccat gggtgaagtc
    14461 aacgcggcgc gcgcccggct ggaggaaatc tacgccgcct acgccgaacc ggacgccgac
    14521 ttcgacgccc tctccgaaga gcagggcaag ctggaggcgg tgatcgccgc cgccggcacc
    14581 gacagcgagc accagctcga aatcgccgcc gacgcgctgc gcctgccgcc ctgggacgcc
    14641 gtgatcggcc agctctccgg cggcgaaaag cgccgcgtgg cgctgtgcaa gctgctgctg
    14701 tccaagcccg acatgctgct gctggacgaa cccaccaacc acctggacgc cgaaagcgtg
    14761 gaatggctgg aaatcttcct caagcgcttt cccggcaccg tggtggccat cacgcacgac
    14821 cgttactttc tggacaacgc cgccgagtgg atcctggagc tcgaccgcgg ccagggcatt
    14881 ccctggaaag gcaactactc cacctggctg gaacagaagg aagcgcgcct ggaacaggaa
    14941 aagaagagcg aagacgcgcg catgaaggcc atgaagaagg agctggaatg ggcgcgccag
    15001 aaccccaagg gtcgccaggc caagagcaag gcgcggctgg cgcgcttcga ggagctgagc
    15061 gactacgaat accagaagcg caacgagacg caggaaatct tcatcccggt ggccgaacgc
    15121 ctgggcacgg aagtgatcga gttcaagagc gtcagcaaga gcttcggtga ccgcctgctg
    15181 atcgacaacc tgagtttcaa gatgccggcc ggcgccatcg tcggcatcat cggtcccaac
    15241 ggcgccggca agtccacgct gttcaagctg atcgccggtc gcgagcagcc cgacagcggc
    15301 gaggtggtga tcggcaagac cgtgcagctg gccttcgtcg accagagccg cgaggcgctg
    15361 gccgacgaca agacggtgtg ggaagacgtc tccggcggcc tggacctgat caccgtcggc
    15421 aagttccaga tgcccagccg ggcctactgc gggcgcttca acttcaacgg ccaggaccag
    15481 cagaagaagg tcggcatgct gtcgggcggc gaacgcggcc gcctgcacct ggccaagacg
    15541 ctgatgcagg gcggcaacgt gctgctgctg gacgagccca gcaacgacct ggacgtcgaa
    15601 accctgcgcg cgctggaaga cgcgctgctc gaattcgccg gcagcgtgat ggtgatcagc
    15661 cacgaccgct ggttcctcga ccgcatctgc acccacatcc tggccgccga gggcgacagc
    15721 cagtgggtgt tcttcgacgg caactaccag gagtacgaag ccgacaagat caagcgcctg
    15781 ggcgaagaag gcgcgcggcc acaccgggtg cgctacaagg ccctcaagtg aggccaggcc
    15841 gcaagacgct gcaggaaagc cgatgggcac ccccgccacc ctgatcgtca ccggcttcga
    15901 cgcgcagcat ttcgatctgg cgcaggattt tgccgactcg tttcgccaag cctaccagga
    15961 acagtaccag ctggccgcgg tcgtgttcgg ccctcaggcg ccgccgcccc agttgtctga
    16021 ccggttcgat cggatcaccc ggcttgacgc cagtggcccg tcgttcgacg ctggaaggaa
    16081 cgggtacttt cttgcccatg ccggagtgaa cgcccgactg cgcgacatct ttccgggcta
    16141 tgccagctac tgctgggtcg acgccgactg ctggtttcag ggcgatgaat ccctgccacg
    16201 catcctggcc ggcatgccgg ggttcgacct ctgcatccac cccgagttcg acgtccacta
    16261 cctgaacttc cccacgccga gcgaacgcac gctccacatc tatcggacca atgaagcggc
    16321 caacctgtcg gccatgccgc tgcgcatgcc catggtcaat gccggcgttt ttgccatgcg
    16381 gaccgactcc cgcgtgtggg ccctgtggga tgccgaactg accgggctgc gccagcggca
    16441 cgagcgcggc gaagcggtat tcttttccga ccagattgcg ctgcacaagc tggtctacct
    16501 gaaccagttg cgcatctaac cgctgcgtgc catcgacaac tggcagacct acgcctgcct
    16561 gccgatgatt caccgggaga cacggtgtct gcgcgtgccc acaccgccgc acgagaaaat
    16621 cggcgtcatg cacctggccg gccgcagcaa acaccagcag ctgcagatcg acgggcagac
    16681 gctgaccctg cgctaccgcg acatggccag gctgttcgcc gcctgacgcc gttcgtcagg
    16741 ccatatccgt cagaaaccct taccgacagt gcgccagcag ctatcgatag tgaagcatgc
    16801 gggcctctgt cgggacgggt tcacttcttc atcggacagg tgttcatgcc cagcagcttg
    16861 taggccgggc aactgcccag caggccggtg agcagcggca ccacgccgat ccagccccac
    16921 cagcccacgc tgccggtggc cgccaggccc accagcacca ggccgacgac gatgcgaaga
    16981 atacgatcga taccacccac gtttttcatg acagactcct tgttgcagtg ttgaatgttt
    17041 gagcgcagaa ggcaggaagg caggccccat gcccgaagcg ggcgccccca cgccgccgat
    17101 ccaggcgcca ggcacggccg cagcctgggc tgcggcgagc atggtcaacg ccagaccggc
    17161 ggttcggtgc gggccaggcg ggcatgaggg attcgttcac accttgtcag cccgctttgc
    17221 cagaacggcg cagcccggtc agcagcggcc agccaaaggc cacaccggcg acgccccagg
    17281 cggcggcgat gcccagcagg ttggcccagt cgacccagta cttcatctcg cccagggcgg
    17341 acttgttcca gatcggcgtg accgcgaaga tcacgatggc caccagcagg cccagcacga
    17401 tggcgccggg caggctggcg cgccagtcgg cgccccggcg accccaggcg cgccccatca
    17461 gcagcagcca cagggcggcc agcgccagca gccagggcca gatcgacagg gccagttcgt
    17521 agacgatgtt cagcatcaac cagatttcat acatgacggc tctccttcgg tcacgggatg
    17581 gtcagacgcg gcccttgagc accgcgatgt aggccggctt gagcaggcgg tacttcatga
    17641 accaggccat gaagctctcc tgcagcggct caatcatggg cagcgagggc gtgagcttgc
    17701 cgtcgtagtc gaactcgatc agcatggccg agccttcgcg cacgatcatc gggcaggagg
    17761 tgtaaccgtc gaagatctgg tcgggcggct gcccgtcgat cacgcgcacc aggttgtgcg
    17821 ccaccagcgg cgcgctcttc ttgacggtgg ccgcggtctt gccgcgcggt gtgccgttga
    17881 tgtcgccgac gccgaacacg ttcgggtagc gcttgtgctg gagcgtgtcc ttgctgacct
    17941 ccagccagcc gccggcggcc atcgggcctt ccttggcggc cagatcgctg cccttgacgg
    18001 cgtcgggcgc gcgcatgggc ggcaccacgt gcaggaagtc gtagccgacc tcctggcgct
    18061 cgccctcggg gctggtgaag aaggccttgc gcgcccccgg atcgatcgcc accagcttgc
    18121 gcaggtaatg cgtctggatg cccagcgcct tccagcgcgc cagcacgttg tcgttgaccg
    18181 gcttgacgcc gaacacggtg tcgtcgggca ggccgctgaa gaagtcgacc ttggacttgt
    18241 ccagcgtgcc ggcctggcgc aggcgatcgg ccaccatgaa ggtcatcttc agcggcgcac
    18301 cggcgcattt gagcggcccg gcgggcagcg tcatcaccgc ctggccgccc ttggcgcgga
    18361 aggcgtccat ggcctgccag gtggccagcg cggcctcggg gctggcgtag acgctgccca
    18421 ggccgttgct gccgatggcc ttcacgtcca tgccctcgat ctggtcgtaa cccagttgca
    18481 ggccggtggc caccaccagg aagtcgtagc cgatcttgcg gccgccggtg gtggtcaggc
    18541 tgttgctggc ggggtcgaac gaagccacca tttcctgcac ccattccacg ccctcgggct
    18601 ggaactcggc gttgcggttc ttcacctttt cgatcggcca gatgccggtc gccaccaggg
    18661 tgtagccggg ctggtagttg tgctcctgct tggcgtcgac gatggtgatc ttggcgcccg
    18721 gcaccagctg acgcaggcgg ctggccacgg caatgccgcc caggccgctg ccggcgatga
    18781 cgatgcgcgc ctggctggaa gaggtctgcg cccgcgcggt cggtgccgcc tgcagcgcgc
    18841 tggccgccag tggcgccgcg cccagcgtgc gcagcaagtc gcgccgcgaa gggcgctgat
    18901 cggatcggtg ggtcatggct tgcctcctgt cttggggttg aactcaggcg cctgccttgc
    18961 gcagcagacg gacgtgcatg gggcgttgga agtgggcgcc ctcgggaccc acgtgggccg
    19021 caaagcgggc ccgcacctgt tcgcgcaagt cgtcgtcgag gtgacgctcg gcataggtca
    19081 catcgagcat gcgacgctcg aaatcggcaa agtcggcgta ccgcacgggc accgcaaaat
    19141 gccgctcggc cgccgcctcc cactggcccg aggccagcgc acggtccagc gccgcctgcg
    19201 ccgcggcgcg cacggcgcct tcgtcgttgt acaggcgggt gatctggttg agcgcgccgg
    19261 catacaccgg ctccgacacg tacagatggc cgccggtccg cagcacgcgg tgcacctcac
    19321 gcagcgcctg gtccatgagc gccagcggca cgtggtgcag cgacttgagc atcagcgcca
    19381 gatcgaactg cgcctcgggc agcggaatcg cctcggcgcc ggcggcgatg aagctcaggc
    19441 gttcggccgg ggcggccagg tttctggcgt gctgaatggc gtccacctcc aggccggtca
    19501 cacgcgcgtc ggggtggcgg gccagcagat cgcgcgccag ccgtgcgctg ccacagccca
    19561 gctcgatgac ctgcagcccg gccagcggca ccagctcggc cagcaggttc agttcgtcgc
    19621 gttcgatgcg cgcttgggac cagtccacgg tgcttccttt catcgatagc gcgccaggta
    19681 gcgccgctcg aagctgcgct tggcccagtg catcagccgg gtcggtggca gcacgatctg
    19741 gcggcgctcg gtgcgcagca ccagggtgcc ttcttccagg gcgtcgatga tgcagagcag
    19801 ctccaccttg aagccctgtg tgcccggctg ccccgccagt tcggacagca ggttgcgcgc
    19861 cgccgcctcg gcctgcaggt ctgccatgtg ggcctgcttg ggcatccagt cgggccccgg
    19921 aaagctgccg ctgtcgccga ccacgtacac ccgctccagc tgcggcaccc ggcaacccgc
    19981 atcggcggcg atcaggccgc ccggcgagcg tggcaggctg gattgctcaa gccaggccgg
    20041 gccggtcagg ccgggcatga acagaatcag atcggccgca aactcgccgc cttcggtgac
    20101 cacgcggtcg gcctcgaagc gcaccatctt gtggcccaga tgggtctgga tgccgcgctg
    20161 cgccatcatg gccagcaggc gcgccaccgt cctgctgccc agccgggcgc caggctgctg
    20221 cgacgggttg aagaacacca gctggaagcg ctcacgccgc ccttcgcgcc gcaactgccg
    20281 atcgatgccg aacaggaact cgaacatcgg cccgccgcgc accgcgctgg gctcgttcgg
    20341 gttggcgcca aagccgacgg cgatggtgcc gccgctcatc tggcgcagcc ggtcacggat
    20401 gcgctcggcg gcgtcgatgc cttcgcaggg agtgatggcg tgctcgatgc ccggcagctt
    20461 gcggatgaag cgcgcaccgc tggcgatcac cagtgcctcg ttggccagct cgccggccga
    20521 ggtgtgcacc acccgcccgt cgtccgacag gccggtgacg ctggccagat ggtggcgcac
    20581 gttcatgcgg gcaaagaagt tgtccagcgg caccaccaga tcggcgcgcg tgcgcaggcc
    20641 actgggtatc cagatgatgc cgggcaggta gtgcagctcg gcgcgcggcg ccaccagcgt
    20701 gatgggcgcc gtacagccct gctggcgcag gctgcgcacg gtggacaggg cgccgaagcc
    20761 ggcgcccagc accacgatct gcggcgcggc cacgccgttc atcggggcac ctccggcgcg
    20821 agcgtggcgt cgacctgcgt cagcagcgct tcgtccagcc gatcggccag ggcggccagc
    20881 cgcaggcgcg tcagcacctg gtcgctgcgc gcctgggcca gcgccagttc ggcgtcggcc
    20941 tgggcgtttt cggcgttcag cacgtcgagc gtggtgcggt cgcccacctc gcgtcccagg
    21001 cgggtggcgt ccaggcgggc gcggctggcc accaggccct cctgcagcgc cgccaggcgc
    21061 gcggcaccgg tctgcaggcc ctgccaggtg gcgcgcacct gctgggccac gtcctcgcgc
    21121 gcgagttcga gttcggccat ggcctgattc aggcgttcgg cggcttcgcg ctcctgggcc
    21181 tcacgcatgc cgccggtgta gagcgggatg ttgagctgca cgcccaccat gccattcagg
    21241 ctgcggttgc gtgccgaacc gaagtcgcca ccgccggcga tgcgttcgtg gcctgcctgc
    21301 gccaccaggt ccagcgtggc gcggcccgcc acgctgtgct ggcgcagctt ctgttcggcc
    21361 agcgccaccg cctgcgcgag caggcgcagg cgcgggttgt cggactcggc ggccgccagc
    21421 cagaacggca gcgcttccac cggcgccagc ggcgccagcg gcagccgggc gcggggttgc
    21481 gccaggccgg tgctgccggc cagccggtcg cgctgcacgc tgagttccag ctcggcggcc
    21541 gactgctgcg cccgcacacc ggccagggcg gcgtcggctt catgggtatc ggtgatcggc
    21601 gaggcgccca gctggtagcg gtcgtgcgcc tcggcgcgcg agcgcgacac ggcctgccac
    21661 tggcgctcga ccacgcgcac gcgctcctgc gccacggcca gggccaggta gcgctcggtg
    21721 gtgcgcagca tcagatcggc ctgcgcgccg cgccaggcga tgtcgcccat gtcggcgccc
    21781 agccgcagct gagcctgctg cgcctgccgc gtggcattga cgagcggctg ctgcgcctgc
    21841 accgccacgc gcgtggccag gccgccggtg accgaggtgg caaagcgcgc ctcgtcgacc
    21901 tggcccatgc cgggcgccga aaaacgcgcg ccccgcatgg cggtgtcctg cacgcccagg
    21961 cccaggccga ggttcaggcc cacctggggg cgccacagcg cgtcggcctg tgcgcgcagc
    22021 gtctggctgg caccgtgttc ggcacgcgcc acggccaggc gctgatcgtg cccggccgca
    22081 gcgcgccaca cggtgagcag gtcctgcgcc cacgcgccga ccggcacgcc gagcgcggca
    22141 cccaccagga ccagacgcgt cagacgcttc atgccgacgc cttttctccg ccgaattcct
    22201 cgtaggcggc cagcgccgag gcagcgtaca tcaggcccgg gccaccgccc atatagacgc
    22261 acatgcccag catttcttcg acctcggcgc gcgtggcgcc cagcttgacc agcgccttgg
    22321 cgtggtagcc gacgcagggg tcgcagcgcg cgcccaccga cagcgccatg gcgatcagct
    22381 ccttggtctt ggcgtccagc gcaccggcct tgctggccgc ctgcgccatg gcgttgaagc
    22441 cctgcatgac ctcggggatg tccttgcgca gcttggccga attggccgac acggcggcga
    22501 tcaggttggt gtagttctcg accatggaat gctcctttca gatgcctgga tgaggttcag
    22561 ctgcagctct cggccggcac gccgagcttg agcaggatgt tgttgagcag gcaccatttg
    22621 gtgaaaccgc tttgcagcag gttggcgccg acgaaggcgg tgaaccagag ccagttgctg
    22681 ctgtggaaga tcgggctgcc ctgcacgccc agggccagcg acagcaggat gaaggtgccc
    22741 gcgaagatac ggacgatacg ccaggaagtc atgtcaattg ctcctattta agtagctgct
    22801 tgcgcaggct gggtgggcgc cagagccgaa ttcttttcat gagtccggcg gtaggcggcg
    22861 aaatacagga tcgggatcac caccagcgtc agcacggtgg acaccaggat gccgaagatc
    22921 agcgatatgg ccagcccgtt gaagatcggg tcgtccagga tgaacagcgc gccgatcatg
    22981 gcggccagcg ccgtcaggcc gatcggctgc gcccggatga tggcgctctg cacgatggcg
    23041 cgcgccagcg gcatgccctc gctcacctgc agcttgatga agtccaccag caggatggag
    23101 ttgcgcacga tgatgccggc cagcgcgatc atgccgatca tgctggtggc ggtgaactgc
    23161 gcgcccagca gcgcatgccc cggcatcacg ccgatgatgg tcagcgggat cggcgccatg
    23221 atgatcagcg gcgtcaggta ggagccgaag tgcgccacca ccagcaggta gatcaacacc
    23281 agcccgaccg cgtaggccgc acccatgtcg cggaaggtct cgtaggtgat ctgccactcg
    23341 ccgtcccact tcagggcgta gccgcggtac gggtcctgtg gctggcgtat gaagtactcg
    23401 tccagccgcc cgccatcggg catcttcagc tcggccaggg cgccgcgcat ctggaacatg
    23461 ccgtacagcg ggctgtccag ctcgccggcc cgctggcccg ccatgtcacc catcacgaag
    23521 ctgaccggca gcaggtcctt gtggtagatc ggctgctcgc gcagcgtatt gctgggcgtg
    23581 accagctcgc ggatcggcac cagagcgccg ctggccgagc gcacgcccag ttgcagcagc
    23641 gcgtccaggc tgccgtgcag ctcggccggc agctgaatga aggccggcac ggcaaagcgg
    23701 ctggcgtcgt gcaggtaggt ggcggcgtcg cccgtcagcc ccatgcgcag cgtgctggcg
    23761 atggcctgct gcgacacgcc caactgcgcc gccttgcgac gatccaccac cagcacggtg
    23821 cgcggcgcat cgacgatggt ggtgtcgtcc acgtcgacca cgccgggcgt ggcctcgaac
    23881 acggcgcgca cctgctgcgc cacggcgcgt cggccgtcgg cgtcggggcc gtagatctcg
    23941 gccacgattg gggccagcac cggcgggccg ggcggcacct cgaccacctt gacgttggcg
    24001 ccatgacgct gaccgatggc gcgcagggca gcgcgctcgc gcacggcgat ggcgtggctc
    24061 tggtccttgc gctgcgcctt gtcgaccagg ttgacctgca ggtcgcccac ttcgccgccg
    24121 gcgcgcaggt agtactggcg caccaggccg ttgaagttga tcggcgccgc ggtgccggcg
    24181 taggcctggt agtgggtgac ctcgggcacg ctggccaggt gggcgcccag ctcgcgcagc
    24241 accgccgccg tcttctcgac cggcgtgccg gccggcatgt cgaccaccac ctggaactcg
    24301 gacttgttgt cgaacggcag catcttcagc accaccagac ccagcaccgg cagcgccacc
    24361 gacagcccga tcaggccggc caccgccagg cccatcaggc ggcgatggcg cgcgccccgg
    24421 cgctcgtcca gcagcggccg gaagatgcgc tcgaacagcg gcgtcagccg ggccgccagg
    24481 ccgtggccgg ccgcctggcc gtggtcggcc tggtgcgtgg ccttcatcca cagccgcgcc
    24541 agccagggcg tgacgctgaa ggcgatcgcc agcgacagca gcatgcccat gctggcattg
    24601 atggggatgg ggctcatgta cggccccatc aagccgctca cgaaggccat cggcaacagc
    24661 gccgcgatga ccgtgaaggt ggccaggatg gtcggcccgc cgacttcgtc gaccgcgccg
    24721 gggatgattt gtgccagcgt gcgctccggg tacaggctct ggtggcggtg gatgttctcg
    24781 accaccacga tagcgtcgtc gaccaagatg ccgatggaaa agatcagcgc gaacagcgac
    24841 acgcgattga gcgtgaagcc ccaggcccag gaggcaaaca gcgtcaccgt cagcgtcagg
    24901 atcaccgcca ccccgacgat ggccgcctcg cgccggccca aggcaaagaa caccagcgcc
    24961 accaccgaca gggtggcaaa cagcagcttc ttgatcagcg tggtggcctt gtcgttggcg
    25021 gtggcgccgt agttgcgcgt ctcggtcacc tgcacgtcgg ccggcagcac cgtgttgcgc
    25081 aactgatcga cgcgccgcag cacggcatcg gcgacgtcga tggcgttctc gcccggcttc
    25141 ttggtgatgg ccagggtgac cgccggctgt tccagcgccg tggccggcgc gccgccgtca
    25201 ccgcgccgca cctcgccatg ccacacatag cgcgtgggcg gcggcgcgcc atcgcgcacc
    25261 tgagccactt cacgcagata caccggcttg ccgccgcgca ccgccagcac cagctcggcc
    25321 acatcctgtg cgctgtgcag gtaggcgccg gtctcgaccg ccagcgcgcg gttgccgccc
    25381 agcagctcgc ccaccggcag accgaggttg gccgactgca gggcgccgtg caactcgtgc
    25441 aggctgaccc cggcggcggc caggcgctgc gggtcggcct cgaccatcac ggcgcggccc
    25501 gggccgccca gggtgtagac ctcgcgcgtg ccgggcacgc gcttgagctc gctctccagg
    25561 ctgtgcgcca cccgctcgag gtcgaacgca ccgctgtcct ggcgcgcgct ggacagggtc
    25621 agcgcgacga tcggcacatc gtccacgccc ttgggcttga cgatgggcgg cagcacgccc
    25681 aggccacgtg gcagccagtc ggcgttggcg ccgaccgcgt cgtacaggcg caccactgcg
    25741 gtctggcgcg gcacgcccac cttgaactgc accgtcagca ccgccacgcc agggcgcgac
    25801 acgctcatca cgtgctcgac gtcgctcatc tgcgacagca catgttcggc cggcgtcgcc
    25861 accatggcct cgacgtcgcg cgcgctggca cccgggaagg gcacgatcac gttggccatg
    25921 gtgacgtcga tctgcggctc ttcctcgcgc ggcgtcacca gcacggcgaa cagaccgagc
    25981 aggaacgcca ccagcgccag cagcggcgtg atctgggcgc tctgaaagta cgccgcaatg
    26041 cgcccggaca cccccaggcg cggcgcgcga tcggcatgca ggggctgtcc catcagcgcc
    26101 ccgccggagc cagccgcacc gccgcctgcg gtgccgtcac cactttgtcg cccgccgaca
    26161 ggccggccag cacctccacc tggtcgccct gcaccgggcc cagccgcacc tggcgcagca
    26221 gcggctgccc ctgctcgccc agcacgtaca ggccagtcat ctcggcgcgc tgcaccagcg
    26281 ccgtgcgcgg cacgaacaga cgctgcggcg cggcagcatc ggcgccaccc gtgccgggca
    26341 gccacaggcg cgcgaacaac cccggcgcca cgccttccag gccgaccggc aactcggcgc
    26401 gcacctgctg cgtcagcgtg cccggatcga ccgtcggcag cacctgcacc cgcgtcgggt
    26461 ggatcggcgc cctggcgcga tcgagctcga tgcgcgcctg ctgcacgccc gccgccacca
    26521 ccgcgctggg caccgccgcg ctgacgcgca gcgccgccgg gtcatacagg gtcagcaagg
    26581 ggctgccggg catggccatc gagccacgct cgaccgacac cgccgctacc acaccgtcaa
    26641 acggtgccgt cacgacgtga aacgccgtct gcgtgcgcgc cgcgcccgcc tgggcctgca
    26701 gcgcattcac ctgcgcctgc gcggcacgct gcgctgcctc ggcctgctcc agggcggcct
    26761 tggcgatgta gttgcgctcg tagagctggc gcttgcgggc cagttcggcc gccgccacct
    26821 ccagtgcggc acgcgcggcc accacctggg cctgactggc cgccgcgccc tgctcggcgg
    26881 cacgggcgtc gatgcgcacc agggtctgtc cggcgcgcac ccggtcgccc gccttcacgt
    26941 tcaactccag cacggcgccg ggcacctggg ccgccagcac cgactggcgc accgcctcca
    27001 ccgtgccttc aaacgcctgg gccgaacgtg cggcctgggc attcaccgtc atgtgcggca
    27061 gctcctgcgc cgacaccggc agcaccagcg cgccgcaggc ggccagcagc agcacacctg
    27121 cagcccgtga gccacgccac gcaaaaaggg gtttgcttgt caccgtcatt gcgatattat
    27181 ttgcgcaatc acgcaaatat gcaatgttgc aaatacccaa cgaccccggg gcaccgctag
    27241 actgcgggga acacccgtcc aacttgcagg tggttttctt ggcctcgatg cagcaacttc
    27301 cccccgaagc gcttgagcat gtggcgcatt actttcgcac gctgtccgag gcaacgcgcc
    27361 tgcgcatcct gaacgtgctg gggcatggcg agatgagcgt tggcgacatc gccgagaagg
    27421 tggattccag cgtcgccaac gtgtcgcgcc atctggcgca gatggcgcag cacggcctgg
    27481 tggcacgtga aagccggggc aacagcgcct actaccgcat cgccgacccg gccgtgcacc
    27541 agttgtgcga tctggtgtgc ggcaacatcg cgcgccgctt cgaggacgtg gccagcaccc
    27601 gcgccgcctt cgtccgcacc gcgcgcgcca aacccacgac cggccgctga acggccaccc
    27661 ggtcagctgg cgcgaaccac cagggcgtcg atcttggtgt gcgacagcac gctttgcgcc
    27721 acgctgccca gcaccagctt ttcgagtccg cggcggccgt gcgaccccat cacgatcagg
    27781 tcggcaccaa cggcgccggc cgcctccaga atgccgcgcc acacggcgtg ggactccacc
    27841 acccggctgt cgacctgcac cccagccgcc tgcagacgct ggttggcggc ttcgatcgcc
    27901 gctcccgcct ccgcccgcgc cgcgttcagg tactggtcct gcccgtaggc gaagtcggcg
    27961 cccacgccgg tgaacgggta gggatcgatc acgaagatcg ccgtcaccgg cacgccgtac
    28021 gcccgcgcca gccccgctgc cttgtcgatg gctgccatcg acgcggtcga tccgtcgacc
    28081 ggaaccagaa tgtgcttgta catgatggac tccttggttg cctatgcgcc tgaaaccatt
    28141 ctctgatcgc atgcccggcc gcagtttgac acgcatcaag tatcagggcc cgtcaagccc
    28201 caacctcccg tcacggcagc acgctgcgga tgcgccgggg cgcgcccgcc cgggttgacg
    28261 acaatcggcg tgctgcccgg cgcgcggcca cctcatgccc atggacacca tcaaccttgc
    28321 actcgtgggc atcagcctgg ggctggccct gtcgtttcga ccctggcggc tgctggcggg
    28381 cggcgccctg ctctcgccct tgctggcatc gctggtcatc acgccctggc tgtgggccct
    28441 gccctggctg caccggatgc cgatccagtt gcagctgtcc ggtgcctgcc tgatcgggct
    28501 ggccctgggc tggccgctgg cggtaccggt gttctgcctg attgcggtgc tggtcggcct
    28561 gatcgcaccg gtgagctggg cacagcagct ggacatggca ctgtggctgg gcatcgtgcc
    28621 ggccaccctg tcgttcggcc tcggcatggt gctgcggcgc tgggtctggc gcaacctgtt
    28681 cgtctacatc ctggggcgcg cgtttctggg caccgcgctg tgcatgttcg ccagcggggc
    28741 cctggcgcag tggacgggtc accgcctggc cgccgcggtg gaccccgacc tggccatggt
    28801 ggcgcgctgg ttgatcgcct ggggggatgc cttcatgacc ggcatgatgg ccgcgatctg
    28861 cgtggccttc aggccacatt ggctggccac ctggtcagac tggctgtaca tcgcaccgcc
    28921 accgtcgccc cgatcgaacc agccctgacg ttgaggcgga tcaagttcgg ccatgtccat
    28981 aacatggata ctcgtcacga tccgatgaac caaagaggaa tgggacgatg aaactgtggc
    29041 aagacctgtt cggcaccgac tacggactga tgagcatcgc cggcatcatc ttcatgatcg
    29101 gcatggcgat ctggttcgtg cgcttctttc tgcgcaagat cgcgcagtcg tccgatcagc
    29161 aatgaactga ccgggcggct tcagcccctg ggcgcctcgg ccgcctccag cagcagcgcg
    29221 gtctgacgcg cgagcaactg cagcgtgcgc agctgcaccg gcgacagcgg gcccgcacgc
    29281 cggtcggcca cccagagcac gccccacacc ttgcccgagg cgccgaccag cggagcgccg
    29341 gcccaggtgc ggatggccag gccctgcgcc agcaaggcgc agccgctgaa ccgcggatca
    29401 ccccccaggt cttcgatcac cgtcacctga tcggcgcagc gcatcgccag ttcgcagggt
    29461 gagccaggca gcggcagcgc ctccacggcg accccggcct gcgctttgaa ccgaagccac
    29521 ggtccatcgc gcagcgccat cgcccccagc ggcatgcggc agatgcgcaa cgccgtctcc
    29581 accagatagg catgggcgtc gtccccccgc gcctgcaggg cggccaggcg ccgctgctcg
    29641 gccgcgcgcc tgggcgccgg cacctgcgcc gacaaggcgg cctgcacggg ggcatcgccc
    29701 ggccgggagg tcaggcgcgc acgcgcctcg ggcggggtcg gcaccttggc ggcggccgcc
    29761 ggggcgctca aaaatctgct gacgaaccac ccccacatgc ccacaccctg aatgtaactt
    29821 tttgttgatt ggtcgcgatt attgcgcatc cgcagccggt cgacaggttt ttttgcgcgg
    29881 cccccgcgcc gccaccggcg gcgacaatcg gcgccatggg tttgcggctc ttgcgacaca
    29941 tggccatcgg tctggccggc atgctggcgc tgggcgccat ggcggcgccg gccttcgacc
    30001 aggtgcgggc cgacttccgg ccctccgaaa ccgtgctgct ggatcgcagc ggccagccgc
    30061 ttcatcgcct gcgcaccgac gcgcgcgtgc gccgcggcga ttgggtgcgg ctggaccagg
    30121 tctcgccggc cctgcgccag gcgctggtgc tgtcggagga caagcgcttc tacgagcaca
    30181 gcggggtcga ctggcacgcc gtgggcgccg ccgcctgggc caacctgtgg cacacgcgca
    30241 cgcgtggcgc ctcgaccttg accatgcagc tggccggcct gctggacgac gacctgcgcc
    30301 tgggcagcgg cggtcgcagc ctgcgccaga aggtcgggca ggtggtgctg gccacgcagc
    30361 tcgaagcgca gtggcgcaag gaccagatcc tggaggccta cctgaacctg gtgccctggc
    30421 gtggcgagct ggtcggcatc gatgcgctct cgcgcaccct gtttgccaag gcgccgcatg
    30481 ggctgcagga cggcgaagcc gccatcgccg ccgccctggt gcgggccccc aacgcccccg
    30541 cggcccaggt cgcgcggcgc gcctgcggcg tgctgcagag catgcgtggc gaccgcgccg
    30601 ccgccgattg cgtggcgctg gacatgcaga ccgaagccgc gctggcgcgc cgcgccttcg
    30661 cgcccagcga cggcatcgcg ccgcactttg cgcgccgcct gctcgccgcc acccgcaacg
    30721 gcagcgaccg cgcccccgcc acgctgccca gcacgctcga cggccggctg cagcgcctgg
    30781 cgctggaggc gctgcaaggc cagctgcgcg aactgcgcgg ccgccatgtg gccgacggcg
    30841 ccgtcatcgt gctggacaac gccagcggcg aggtgctggc ctgggtgggc tccagcggtg
    30901 cgctgagcag cgccgccgag gtcgatggcg tgctcgcccc acgccagccc ggcagtacgc
    30961 tgaaaccctt tttgtacgcg caggccatcg ccgagcggcg catcacggcc gcgtcgctgc
    31021 tgcacgattc gcccgcgcaa ctgcacaccg ccagcggcct gtacatcccg cgcaactacg
    31081 acctgcagtt caagggctgg gtgtcggcac gcaccgcgct ggccgcctcg ctcaacgtgc
    31141 cggcggtgcg cgtgggcgtg atggtctcgc cgcgcgcctt tgcgcagcaa ctggggcggc
    31201 tgggcattga gctggccgaa agcggcgact actacggcta cagcctggcc ctgggcagcg
    31261 ccgaggtcag cctactgcag ctcaccaacg cctatcgcgc gctggccaat ggcgggcgcc
    31321 tgtgtccggt gcggctgctg cccaatgccc ccggatcact ccctctccct ctgggagagg
    31381 gccggggtga gaccgaaact cactctcgcg ccgcgagcct ttcactccct ctcccgtctg
    31441 tgggagaggg tcggggtgag ggcaaccgga gcccgcaggt cactgccacc acgccctgca
    31501 cgcaggccct cgacgccggc gccgccttca tcaccagcga catcctgtcc gaccgcaacg
    31561 cccgcgcccc caccttcggc accgactcga tcctggccac gcgcttctgg agcgccgtca
    31621 agaccggcac cagcaaggac atgcgcgaca actgggcggt cggctacagc cgccgctaca
    31681 ccgtcggcgt atgggtcggc aacgccagcg gcgccgccat gtgggacgtc agcggcacca
    31741 gcggcgccgc ccccgtctgg gccacgctga tgcgcgagct gcaccggcac accccctcac
    31801 cgccccccgc cccacccgct ggcctacagc acgtcgccgt gcactacgcc gccggcctgc
    31861 aggcgccgcg cagcgagtgg ttcctgcccg gcaccgcaca gagtgaattc gctatagaaa
    31921 cagaagctgc cagcgcagac ccagcaaagg ctggaggcca aaaaggcttg aaaacggcca
    31981 gccaccccag cgcccccgcc ggcgtgcccc cgcgcatcac cgcgccgcaa gacggcacca
    32041 tcctcgccct cgaccccgac atcccccccg cccatcagcg cctgcgcctg gccgccgaac
    32101 ccggcagcgc ccccgccaac agcctgcgct ggtgggtgga cgatcagccc attggccgtg
    32161 gcgcgcaggc cgactggctg ccgatgccgg gcaggcatcg gatcaccttg tgcgatgggc
    32221 gcgggcgggt ggtggatgaa aggcggatcg aagtgcgggg cgcccagccc cgccatcccc
    32281 gctcagccgg ggccgccgat cgacttgcga aacaataggg cccccacgag gaacaccgtg
    32341 tttgaacaaa ccttcaagaa catcgacgac atcctgcaca aggacgccgg ctgcaccagc
    32401 gaactggact acaccgagca aagctcctgg ctgctgtttc tcaaatacct ggacgacctg
    32461 gaggccgaca aggccgccga ggccgagctg gccggcaagc cctaccgcta catcctcgac
    32521 gcaccctacc gctggtcgag ctgggccgcc ccgcaagatg cggccggcgc gctggaccac
    32581 aacagcgccc tgaccggcga cgacctgcgc gacttcgtca accagcggct gttcccgtac
    32641 ctggcgggct tcaaggcccg cgccagcggc cccaacacca tcgaatacaa gattggcgag
    32701 gtctttggcg agatcaagaa caagctgcaa agcggctaca ccctgcgcga cgtgatcgag
    32761 caggtggacg cgctgcgctt tcgctcgcag accgagaagc acgagctctc gcatctgtac
    32821 gaagccaaga tccgcaacat gggcaacgcc ggtcgcaacg gcggcgagta ctacaccccc
    32881 cgcccgctga tccgcgccat gatccaagtg accaagcccc gaatcggcga gcgcatctac
    32941 gacggcgcct gcggatcggc cggctttctg tgcgaggcgt tcgattacct cagaagccag
    33001 ccgggcctga ccaccgccga cctgcagacg ctgcagcagc gcagcttcta cggcaaggag
    33061 aaaaagagcc tggcctacgt gatcgcgatc atgaacatga tcctgcacgg catcgaggcg
    33121 cccaacatca tccacaccaa caccctggcc gagaacctgg ccgacatcca ggaccaggac
    33181 cgctacgaca tcgtgctcgc caacccgccc ttcggcggca aggagcgcaa ggaggtgcag
    33241 cagaacttcc cgatcaagac cggggaaacc gccttcctgt ttttgcagca cttcatcaag
    33301 agcctcaagg ccggcggccg cgccgccatc gtcatcaaga acaccttcct ctccaacacc
    33361 gacaacgcct cggtcagcct gcgcaagctg ctgctggagg gctgcaacct gcacaccgtg
    33421 ctcgactgcc ccggcggcac cttccagggc gcgggcgtca agaccgtggt gctgttcttt
    33481 gaaaaaggcg cgcccacgcg cagcatctgg tactaccagc tcgacccggg ccgcagcctg
    33541 ggcaagacca acccgctcaa cgacgccgac ctggccgagt tcgtgacgct acaggccagc
    33601 tttgccgatt cagcgcaaag ctggtcggtg gatgccagcc gcatagacgc cgccagctgc
    33661 gacctgtcgg tcaggaaccc caatggcggc gaggccgtgg cgctgcgcag cccgcacgaa
    33721 atcctggacg agatcgcggc actcgatgcc gagagtgccg aggcgctggc gaggattcgg
    33781 gagttggtgg catgaatggg agatggccag ttcgaccctt gggctcacta tgccgcattg
    33841 cacgaggagg ctcgccgcgc ccgattcaag cgttcttgac cgatactgat gacgggatca
    33901 attggatcaa gatcagcgat gcgacggcga gtggaaagta catttattcc acagttgaga
    33961 agatcaaacc ttcaggtgtg tcgagatcac gcatggtgaa ggaaggggat ttcctacttt
    34021 cgaattccat gagctttggc cgcccataca tcatgcggac gatggggtgc atccatgacg
    34081 gctggctcgt tctttctggg taccagaatt tcttcgatca agattttttg taccacttac
    34141 tcggatcacc acaagtcttc caacagttca atcaacttgc agctggatcg actgtcagaa
    34201 acctgaacat tgatctggcc agccgcgttc agcttcctat tccatcgctc cccgaacagc
    34261 gccgcatcgt cgccatcctt gacgaagcct tcgacgccat cgccaccgcc aaggccaata
    34321 ctgaggcgaa ttggcagggt gcaagggcgg tgctcaacca atgtcgggat gacgcgctgt
    34381 cctccttggc ggccgagttc ggcaccgtga atctcggttc agtttgcgat tttgagaatg
    34441 gagacagagg caaaaactac cctggaaaac aacaccgtgt ggctgaagga gtgcctttta
    34501 tcaacgctgg tcatctcacc gaagatggaa tcgactttga atcaatggac tacatctctc
    34561 ctgagcgtta ttcgctgcta gggaatggca agattaagcc aagagatatc ctgttttgct
    34621 tgcgaggttc tctggggaaa tttgctaacg ttggcgatac ccccgttggg gcaattgcat
    34681 cgtcactagt cattttgaga ccgagaagtc cgctctacga caagtattta gaaatctatc
    34741 ttgcgagcag ttgttgcatt gaaatgatca acaaatacaa aggcggcgcg gcacaaccga
    34801 acttgggtgc caaagactta aagcagttcg tgcttccgct tcctccacag agcctgcagc
    34861 ggcaactaac tgagcgactt gacggaatta aagaacagac ccatctccta gaggcaatct
    34921 actcagagaa atctgccgcc ctcgacgaac tcaagaaatc cctgctgcac ctggccttca
    34981 ccggccaact gaccgcccgg gccgccgacc ggcaactgca ggccgtggca tgaacgaggc
    35041 cgaaacccgc gccgagcaca tcgaccctgc cctggcggcg gcgggctggg gcgtggtgga
    35101 gggcagccgc atccgccgcg aggtgatcgc ccccggccgc ctgcaggggc gcggccagcg
    35161 cgcgcgggcc gacattgccg actatgtgct gatctaccgc aacaccaagc tggcggtggt
    35221 cgaggccaag gcctgggaca agccccacac cgaggggctg gcgcaggcca agcgctacgc
    35281 ggccaagctg gcgctgcgct ttgcctatgc caccaatggc cagcagatct acggcgtgga
    35341 catggccaca ggtgccgagg gcgacgtgcc ggccttcccc ggcccggatg cgctgtgggc
    35401 gcagaccttt gcccaggcca atgcctggcg cgaccgcttt gccgccgtgc cgttcgagga
    35461 caagggcggc gcgtgggaaa gccgctatta ccaggagatc gcgatccgcc gcgtgctgga
    35521 ggccatcgcc ggcgggcaaa gccgcctcct gctgacgctg gccaccggca cgggcaagac
    35581 ggcgattgcc tttcagatcg cgtggaagct ctaccacagc cgctggaacc tgggcgactg
    35641 gcaggcgccg ggcgcgccca cgcggcgccc gcgcattttg tttctggccg accgcaacat
    35701 cctggccgac caggcctaca acgccttcag cgcctttgcc gacgatgcgc tggtgcgcat
    35761 cgcgccgggc gaaatccgca agaagggccg cgtgcccagg aacggcagcg tcttcttcac
    35821 catcttccag accttcatga gcgggccggg cggcgcgccg catttcggcg cgtacccgcc
    35881 ggacttcttc gacttcatcg tcatcgacga atgccaccgc ggcggcgcca gcgacgagag
    35941 ccagtggcgc gccatcctgg agcactttgc cccggcggtg cagctgggcc tgacggccac
    36001 gcccaggcgg cgcagcaacg cagacaccta cgcctacttc ggcgagccgg tctacaccta
    36061 ttcgctgaaa gagggcatca acgacggtta cctcacgccc ttcaaggtgc ggcagttcgc
    36121 caccacgctg gacgactaca cctacacccc cgacgacgcc gtggtggaag gcgaggtggt
    36181 gcccggcaag cgctatgtcg agggcgagtt caaccgcgtg atcgtgatcc gcgagcgcga
    36241 gcaataccgc gtggggctct tcatgggcat gatcgatccg cgcgaaaaga cgctggtgtt
    36301 ctgcgccacg caggagcacg cgctgatggt gcgcgacctg atcaaccagg ccaagaccag
    36361 ccccgacccg aactactgca tgcgcgtgac ggcggacgat ggggcgatgg gcgagcagtt
    36421 cctgcgcgac tttcaggaca acgagaagtc catccccacc atcctcacca cctcgcagaa
    36481 gctctcgacc ggcgtcgatg cgcgcaacgt gcgcaacatc gtgctgatgc ggccggtggc
    36541 gtcgatgatc gagttcaagc agatcatcgg gcgcggcacg cgcctgttcg acggcaagga
    36601 ctacttcacg atctacgact tcgtcaaggc gcaccaccat ttcagcgacc cggagtggga
    36661 cggcgagccg gtggagccgg agccgccgcg cccgcgtgcg ccggcgccga ccgccgaccc
    36721 gggcgaaccg cctgccgtga ccgaagtgcc gcccgcgtac aggatcagga tcaaactggc
    36781 cgacggcaag gcgcgcgcga tccagagcat gacggccacc agcttctggg gcgcggacgg
    36841 ccggccgctg tcggccgcgc agttccttga aatgctgttc ggcgcgctgc ccgagttctt
    36901 caaggacgag gacgaattgc gcgccctctg gagcgacccg gcaacgcgca gaaggctgct
    36961 ggaccagctg gccgagcgcg gcttcggccg cgagccgctg gccgagatgc agaagatcat
    37021 cgaggccgag cacagcgacc tgttcgacgt gctggcctat gtggcctttg ccacccagcc
    37081 caggacccgc gcccagcgcg ccgacgccgc caaggccgcc gccgcgcccg gcctgagcga
    37141 caagcagcag gccttcgtcg atttcgtgct ggcgcagtac gtccagcagg gcgtggacga
    37201 gctcgatcag gacaagctct cgcccctgct gcgcctgaag taccgcgcgc tgcccgacgc
    37261 ctttgccgaa ctcggccggc ccgagcagat tcgcggcgtc tttgtggacc tgcaacggca
    37321 tctgtacctg gattgagagc ggccccggct gccgcgtgcc aggccgggcg gcaccgcgca
    37381 aggcgcagcg ctggcgttct tccactggcc atcccgcgtg ccgcgcaggg cgcttgtccg
    37441 agtcccattt tgggactaaa ctgcaaaccg tgcgagtgat tgccgtgtct acccttcggg
    37501 ccttctggca gcgccacccc gacgccgagc agccgttgaa cgcctggtac gaggaagcca
    37561 cgagcgcgac ctggacccag cccgccgaca tcaaggcgcg ctaccgcagc gccagtgtgc
    37621 tgaagaaccg tcgtgtggtc ttcaacatca agggcaatga ctaccggctg atcgtggcca
    37681 ttgcgtacag gttgcagatc gtctacgtga aatttgtcgg cacccatcag gagtacgacg
    37741 ccgttgatgc agaaaccatt gaatcggcct gagcggccac ggagaacccc atggacattc
    37801 gccccatccg caccgaagca gactaccagg ccactctcaa ggagatttcg ggcttgatgg
    37861 cttgcgaccc cgagctgggt acgccagagg gtgatcgcct cgacattctg gccacgctgg
    37921 tacaagccta tgaagccaag cactttgcca tcggcgcgcc cgacccggtg gaggccatca
    37981 aattccggat ggagcaaagc ggtctgtccg tcaaggacct ggagccgctc atcggcaaga
    38041 gcaaccgcgt ctacgaagtc ctgaaccgca aacgcccgct gacgctggcc atgatccgcc
    38101 ggttgcacca aagcctgggc atcccggccg atgtgctgat tgcggagacc gcggccgggt
    38161 gatcggcggc ctgggtaacg gggtcagatg ggaaacgggg ggaaacgggg tcagatgaaa
    38221 attttctccc aagccgggcc agtgtcccca ccaggccggc cgacgcagtc ttcgaccggc
    38281 tccaggccaa gtacgccggc aagtccaagc gctgatgcaa ctggccttca cgccgctggt
    38341 cgagcacaag cttgaggcga ttgccgactg aatcccggcc aacatccgcc gggccacacg
    38401 ggttgcagca acctttattc ggcaccgccg ggtttcaccc gcccgccacc gccggacact
    38461 cctgccgcag cagccgcgcc agctcgcgct tgcgctcgat cggcaggcgc ggcgtgatgg
    38521 cgccaaaact cagcgccgcg atggctccgg gcgcggcggc aaaggtgacg cccacgcccg
    38581 acacgccggc ggtcagggtg tcggtgatgt cggcccagcc ctgggcgcgg gtctggtgca
    38641 cggcgcgcag caggcgctcg cgcgtgaagt cggcctgcgc gtattccgcc gcgttgcgct
    38701 cgtagatgcg cagcacctcc gcatcgggca ggcgcgccat gatggccagg ccgcccgcgc
    38761 ccaggcccag caggcgccgc ccgccaatgt cggtggtaaa caccttgacc ggaaacggcc
    38821 cctcctcgcg gtgcaggcac aggcagtggt cgccctggcg gatgaccaga aacaccgtgt
    38881 cgcccgacat gcgcgccagc cgctgcatca gcgcgcggca ctggtcgacc agcggcacgc
    38941 gccgcatggc ggcaaagccc agctgcatgg cgtccacccc cagccgatag accttgccac
    39001 ccgcgtcgcg ctcggcgaac tgctcctcga ccaggcaggc cagcaggcgg tggcaggtgg
    39061 agcgctgcag gcccgaggcg gccatggcct cgctcaggcg gatgccgtct tcctgatgct
    39121 cggccagcag gcgcagcagt tgcagcccac ggcgcaggct ttgagcccct gcggtcgatc
    39181 tgtccatgat gtgggaattt tttaataaat tgtttgcagt gcaggaatgg tacttcctag
    39241 actgcggcca agccacccca cccctacccg agattgacac ccatggcatt tgacaccctg
    39301 gcgatcgaaa cccacggcgc ggtgcgccgc atcctgctgg cgcgaccggc gcagcgcaac
    39361 gcgcaaaact ggcagctgct cgacgagctg atggccgcgc tggatcaggc gcgcaccgac
    39421 gacgccatcc acgccgtggt gctgggcggg caaggcgagc atttctcggc cgggcacgac
    39481 ctcaagcagg cgcaggccga gcgcgccaac ttcacggtcg agcagcgctg ggcgttcgag
    39541 gagctgcgct atttcgacta ctcgatgaag atctgggact ttcccaagcc caccatcgcc
    39601 caggtgcagg gcgcctgcgt ggccggcggc ttcatgatcg ccaacatgtg cgacctggtg
    39661 gtggccggcg agagcgcgta tttttccgac ccggtgggcc acaccctggg cgcggcggcc
    39721 accgaggtgc tgatccaccc ctgggtgatg ggcgcgcgca aggccaagga gctgctgttc
    39781 accggcgcgc gcatgagcgc gcaggaggcg caggccatcg gcatggtcaa ccgtgtggtg
    39841 cccgacgccg agctggccga cgccacgctg gcgctggcgc agcagatcgc caaggcaccg
    39901 ccctttggcc tgcgcctgat caagcgctcg atcaaccgca cgctggacgc gcagggcctg
    39961 cgcagcgcgc tggcggcgca cttcgacacg caccagctgt cgcacctgag cgatggcttt
    40021 ctgagcgcgc gcgaccgcgg cctggcaagc gccatccaga agaacccggc caaggcgcag
    40081 cagtgaccgg catgcatcgt ctgcaacccc tgctcgaccc ggcctcgatc gccatcgtcg
    40141 gcgccagcaa caacccggcg cgcatcggcg gcatgccgat cgcccatttg acgaagttcg
    40201 gctacgccgg cgcgatctac ccgatcaacc ccaagtacca ggaggtgttt ggcctgcgct
    40261 gctggcccga catcgaagcg ctgcccgccg cgcccgatct ggtggtgctg gccattgccg
    40321 cgcccgaggt gctgcccatg ctgcgccgct gcgcggccaa gggcgtgcgc gccgtcatcg
    40381 tctatgcggc gggctttgcc gaagaaggcg agcgcggcgc ggcgctgcag gccgagctgg
    40441 ccgcctacgt ggccagcacc gacatggtgg tggccggccc caacgccatg ggctttgcca
    40501 acctcaacac ccaggcgcac accaactttg cctcggtatt caacaccgcg cccatgcagg
    40561 cgggcacggg cagcgtcagc ctgctgacgc aaagcggcaa cgtgtgcgcg gcggtgtacg
    40621 ccatcgcgcg ccggctgggc gtggccttca gtcacttcat caacaccggc aacgaagcct
    40681 gcgtggactt tgcccagtac ctggagtacc tggcgcagga cgaggccacc gacgtctgca
    40741 tcggctacat cgaggagctg cgcgacggcg cgcgtttcat cgccgccgcc gaggaatttg
    40801 cgcggcgcga caagctgctc atcctgtaca aggcgggcga gaccgacaag ggcagcgagg
    40861 cggtgcgctc gcacacctcg gcgctggcgg gcgaccaggc catttaccgc gctgcctttc
    40921 gtcagctcga catcatccag agccacgact tcgtgcagat ggcgcagctg gcgcatctgg
    40981 ccagctaccg ccggcgccgc gccggcaagc gggtggccat catcaccatc agcggggcgc
    41041 tgggcgccat cctggccgac aagttcatcg gcgccgggct ggaagtgccc accctgcccg
    41101 ccgagctgca gcagcagctg cgcgccggca tcccggacta cggcatggtc gccaatccgg
    41161 tggacgtgac cggcaacgtg gtcaacgacc cgggcttcgt gcgcaccgcg ctggaagcgc
    41221 tggccaccag cgacgcggtg gacacggtgg tgatctacgc ccccggctac atgctcgacc
    41281 gcatggccgg cgacctgtgc gccgtcgcgg ccgcgcaccc caggctgttc gtggccatcg
    41341 acaccggcgc cgcccagagc cgcgagcagc tgcgcgccgg cggcgtgccg gtgttcgagg
    41401 acatcggcgt ggccaccgcc gcgctggcgc cctttctgct gtggcagcag cagcgccagc
    41461 gcaaccgctg gcgggcgctg cgccaccagg cgccaccgcc cgccaccgcg ctgcccgagg
    41521 tgctggatga gcaccagtgc aaggccgtgc tggccgccag cggcgtgccc gccgcgcccg
    41581 agcgccgcgc gcgcagcgcc gacgaggcgg tggccgcggc cgacgccctg ggctacccgg
    41641 tggcgctcaa ggtgctgtcg cccgacatcg cccacaagac cgaagccggc ggcgtgcgcc
    41701 tgcacctggc cgatggcgcg caggtgcgcg ccgccttcga ccagatcctg gccagcgcgc
    41761 gcgcccacgc gccgcaggcg cgcctcgacg gcgtgctggt ggcgcccatg cagtctggcg
    41821 tcgccgagct gatcgtcggc gccacgcgcg acccggtgtt tggcatggcg ctcaccgtcg
    41881 gcctgggcgg cgtgctgacc gagctgtacc aggacgtcag ccaccgcctg ctgccggtgg
    41941 acgaggccat ggccgccgac atgctggccg agctcaaggc ctatccgctg ctgaccggct
    42001 accgcggccg cccgccggcc gatgtcgatg cggcctgtgc cgccattgcc gcgttctcgc
    42061 gcgccgtgca ggcgctgggc cggcaggtga gcgaagccga ggtcaacccg ctgctcatca
    42121 aagccagcgg ccagggcgcc gccatgctgg acgcgctgct gctggccccc gccgattgaa
    42181 tttcaaccca ccccaacccc aaggagactc accatgatca agcaactgct ggccggcgtg
    42241 gcgctggccg ctgcggccct gtgcaccccg gcacaggccc agggctaccc cagcaaaccg
    42301 gtcaacatcg tcgtgccgtt cgcccccggt ggcgccaccg acatcatgac gcgcctgctg
    42361 gccgaacgcc tgagcaagcg cctgggccag cccgtgatcg tcgagaacaa gcccggcgcc
    42421 ggcaccatga tcgcgtccga gtacgtggcc aaggcgccgg ccgatggcca caccgtgctg
    42481 atggcggcgt cgtcgctggg catcgcgccc tcgctctaca gcaaggtcaa ttacgacccg
    42541 gtcaagagct tcgcgccgat ctcgctggtg gcctcggtgg tgcacgtgct ggaagtgcac
    42601 cccagcgtgc cggtcaagag cgtcggcgag ctgatcacct ggctcaaggc caatccgggc
    42661 aaggccaact acggctcggt cggcgccggc acctccaccc acctggaatc cgagctgttc
    42721 aacaccatgg ccggtgtgaa gatggtgcac gtcccttaca aaggcagcgc cccggcgctg
    42781 acggacctgg tgggtggcag cctgcaggtg atgttcgacg cctgggcctc gtccggcccc
    42841 ttcgtcaagg acggcaagac gcgcctgctg gccgtcacca cggcgcagcg ctccaggatc
    42901 ctgcccgacg tgcccaccgt ggccgaatcc ggcctgcccg gctacgaagc catgccctgg
    42961 ctgggcctgc tggcgccggc cggcacgccc gacgccgtgg tcaggaagtt ccacgacgag
    43021 ttgatcgagg tgctcaagga acagccggtg caggagaagt tccacagcct gggcctggac
    43081 atcatcggca acacgccgca ggagtttgcc gagttcatca agaaggacat caccaagtgg
    43141 gccaaggtga tccaggaaac cggcgccaag gccaactgaa gccttcgaaa actattgttt
    43201 tgatagctgc tgacgctcac tctacgggcg ctgcggcttg atcgtcaagg aattccgatg
    43261 caggtcggct acgccgccga ggacgaagcc tttcgcgccg agttgcgcgc gtggttcgag
    43321 acccactacc cgcgcttcgt cgccgcctgg ccccaggcgc ccgaccccaa cgccctggac
    43381 tggcgtcgcg cctgggaaga ctacgtctgc caggccggct ggtccggcct gggctggccg
    43441 ctggcctacg gcggcaaggc ctggtcgctc acgcgccagg ccatctttca tgaagagcag
    43501 gcgcgcatcg gcgcgccgct gggcgtcaat ctgatcggcc acggcattct ggcgcccacg
    43561 ctgatccact tcgccagcga ggcgcaaaag cagcgctacc tgcctggcat cttgtccaac
    43621 cgcgaggtct ggtgccaggg ctattccgaa ccgggcgcgg gctccgatct ggcggcgctt
    43681 ggcacgcgcg ccgagcgccg cggcgaccac tacctgctga acggccacaa gatctggacc
    43741 tcgttcgcgc acatcgccga cttctgcttc gtgctggcgc gcaccgaccc cgcggcccag
    43801 cgccacaagg gcatcagctt tctgctggtg gacatgaaaa gccccggcgt gcgcgtgcag
    43861 ccgatccgcc agatcaccgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43981 ntgcgcgcgc tcacgctggc gctgcgcggc ggcccgcccg gcgccgaggg ctcggccacc
    44041 aagctgcact ggagcgaggc gcaccagcgt ctgaccagca cagcggccga actgctgggc
    44101 gagcgcgccc tggctggccc cacgcccggc gggcacgacc cggcggccgc cctgtgggcg
    44161 cgcgagtttt tgtggacgcg cgccgaaacc attctggccg gcacctccga agtgcaacgc
    44221 aatatcctgg ccgagcagat gctgggcctg cccaggtaag cccatggact ttgccctgaa
    44281 cgaagcgcag cagatgctgc aggcgtcggc gcgccgcttt cttgccgaca accaccccct
    44341 gacccgcgcg cgccaggcct tgccctggag cgacgccgcc cagcagcgcc tgtgggccga
    44401 catggccgac atgggctttc tggccctgct gttgcccgaa gatcagggcg gcctggccct
    44461 gggcctgacc gaagccagcc tgctggccga ggaagccggc caccagctgc tcaacctgcc
    44521 ctgggccgcc agcgccgtct ggctggcgct ggcctgccgc gccacgcccg ccagcgtgcc
    44581 cgcgcccctg cgcgacgcca tgcaggccat gctggccggc cggcgcgccc tgcacgtggt
    44641 cgatgcgggc gatggctggt gggaccacgc cggtcagtgc agcgacatcg tcgtgattcg
    44701 cgaaagcccc gcgccgggcg agccgctgca gctggcgctg atcgacggcg ccgccgtgac
    44761 cgccgcgccc gcgctcgacc ccaccctgcg ccaggctccg gcgctggcgc ctactgggcc
    44821 ctggcacgcc tgggcgctgg acgccgccag ccgcgcccag gcgcgcaccg gctaccggct
    44881 gatgcgcgcg gccgagctgg tcggcgtggc tcaggctgcg ctcgaactgg ccgccggcta
    44941 cgccagggag cgcgtgcagt ttggccgccc gatcggcagc aaccaggcca tcaagcacca
    45001 gctggccaac gcctggatgg gcgtggacaa cgcgcgcctg gccgtgctgt acgccaccgc
    45061 cgcgctgaac gagcgcctgc ccgacgcccg cttggcctgc gccgccgccg aggcgagcgc
    45121 catcgagggc gcgctgcagg ccacccgcag cgccctgcag gtgcacggcg gcatgggctt
    45181 cacctgggag agcgacgtgc acctgtacct caagcgcgcc cagcacctgg ccacccgtct
    45241 gggcggcgcc agccgcgcgt tgcagcagct ggaggcgctg gcctgggatg ccgcggcgaa
    45301 tgcgccatct gcgacaccgg tttgaaggcg tggatccgtg tcgcgccgcc gatggtcgaa
    45361 aacccgacct cggcgctgac atgactacag catcgcactc gaattgccat tgtttcgata
    45421 gcgttcggcg cagtccagaa aagcgccaga agccaatttc aatcggattg tcggtcaccg
    45481 cccggcacca caccggcccc ttgcggtggt aggccgcgct ccgacccaaa gccccggcgt
    45541 ccgcgctatc gtgcctcgcc cgccagcgcc agctgccgtg acgaagcctg tcgcgcggcc
    45601 tgcaccacca gttccgccag gcgcttgacg ccacgcgccc ggatgcgctc tgccgggcct
    45661 gagatgccga gcgccgccac gacgcgctgg cgttcatcga acaccggcgc cgcaatgccc
    45721 cagacgctgg cgcgccattc gcctcggttg acggcgtaac cctgctgccg cacgcgatcg
    45781 agctccatcg ccagcgccga agtgtcggcc agcgtggtcg gcgtgtagac ctgcaggggc
    45841 cgcacggtca tcagctgaac cgccggctgt tcgcgccagg ccagcagcgc cttgcccgtg
    45901 gccacgcaat aggccggtgc gcggcccccg atgacgctgt aggcgcgcac cggctcaggg
    45961 ctgtcgagct tgtcgatgta gatcacctcg tcgccactga gcaccgacag atgcaccgtt
    46021 tcgttggttt ccgccagcag catgcgcatg gccggcgctg cggtttcacg gaaatccagt
    46081 ttgtgcagcg cctgcgcccc cagctcccac accttgagcg aggcccgata accaccacgc
    46141 ccgccctcgt tgacggcata gccgagctcg accagggcct gcagcaagcg gtgcacgttg
    46201 ctggcgccca tctccagctc ctgggccagc tcgctgattc ccacgggctg tgcgcgtgca
    46261 accagcacct cgagcaagcg cagccccttg atcagagtgt tgttcattcg atcaccgccc
    46321 tcaatttcta tatgtcggaa caattatctc atttacagaa tttttcaaca cagcttccga
    46381 atcggcaacg ggcacccgcg atccgcatgt tgtggccgtc accgctcccg catggccacg
    46441 ccaacccgtg ccatcgcccc tgacctgcgc acattggccg cgggagtgcg ccttccagcc
    46501 ccctgcgcgg cagacacatg gcaccggtca cggcatcaac gggcccggga ccgcgctgaa
    46561 cgctggcgct cacggcccgg tgcagcccga taggtcttgg ggctgcatcc catttcgcgc
    46621 gtgaacatgt aggaaaacgc ggacggtgaa ccgtagccca gttcgagcgc cgtcgccgtc
    46681 acgctcatgc cggcgcccag cagttccatc gacttgaaca ggcgcaggcg ccgcttccat
    46741 tcgcgaaagt tcatgcccag ttcggcctgg aaacggcgcg tcagcgtgcg ctccgacgca
    46801 cccaggcgcg cgccccatgt ggcgatgtca tcggcgtcgg ccggcgcacg atcgatcgcc
    46861 tcgcacagct tgcgcaacat gggagactgc ggccagggca acggcgcctg aacgcgttcc
    46921 agccgcggca gttgatcgag gatcagaccg tgcacccgtt gcgtataggc cagtgtggcg
    46981 acgcccgcca cacgctgctc gcccagccac gccgcctcac ggatcaactc gcgcagcaag
    47041 gcagacacct gcagcaacgc atagcgcgcc gccatgcccc gacccacgga agtgcgcaaa
    47101 tacagactgc gaaattgcgc gccgtagcgc gatgcgaccg cgtgcacggt accggtcgga
    47161 atccacaccg cctgctcggg cccgcagacc acgcgctgat gggcggcctc gacgaccagt
    47221 gatccgctga tggcatagat gagttgatgc catggatgcg agtgagacgg gaatcggtag
    47281 cccggcggga tgtcctgcgc gcgcacgaac accggcccgg gcaaggtctc gatttccggc
    47341 accttcagcg tcttccacag catcgcaagc cttgagcaac cccttgtcgc ttcatcgaca
    47401 tattctgtct ctttatcgaa agcgcacaag ccggccgctg cctacagtct ccacatgcga
    47461 gacacagaag cttggtcgac aacgcctcgc gcgatgcctg cgcatatcga accgccgcgc
    47521 cgcatcgggt ttgcggccac tgcgctgttc atttacctga tcctgctggc cggctatgtg
    47581 ctgtcctacg tgcaccgcac ggcgccggcg gcgatcgcag gcgagctgac ccaggccttc
    47641 caggtcagcg gcgcgctgct cggcacgctc gccgcgacct acttctacgt ctacaccgta
    47701 ttgcaggtgc cggtgggggt gctcgccgat accgtcggcc cacgcgttgt ggtcactgcc
    47761 ggcgcagtgg tggccgccgt cggctcgatc atgttcgggc tggcgcccgg ctgggaactg
    47821 gccgccgtcg gccgcttgct ggtgggcatg ggcgtggcgg tgaccttcgt ggccctgctc
    47881 aaggtgtgtg ccaactggtt tccaccacaa cgctttgcaa ccttcaacgg catcaccctg
    47941 cttgccggca acctgggcgc cgcggccgcc ggcgcacccc tggcctggct ggtgacggtg
    48001 gcgtcctggc gatcggtgtt cgtcggcctg ggcctggcct cgctcgcgct ggccgtgctg
    48061 acatggatcg tggtacgcga cagtccccgc gagtgcggat tcgaggtgtc ctacgccccg
    48121 ccgcccgcgg tcgcaacgca ctgggcacag gcgctgcgcg ccgtgctggc caatccggcc
    48181 agctggccag gcttcttcgt caacgttggg gtggccggca gcttcttcgc ctttgccggc
    48241 ctgtgggtgg tgccctacct gcgcgaaggc cgcggcctgt cgcaggcggt ggccagccat
    48301 cacgccagcc tgctggtgct cggcgtggcg gccggctcgc tggtggttgg tctgctgtcc
    48361 gatcgtctgc gcagccgcat cggcgtgatg cgcttgttcg ccctgatgta cgcgctctcg
    48421 tggctgcccc tggtgctgcg atgggaaccg tcgctggcgc tcagctacgc ctggttcttc
    48481 gtcatgggcc tgttcgtgcc ggccttcgtg ttgacgtgga ctgtagcgaa ggaaatgaac
    48541 ccgcctgaac acgcaggcat ggccgtctcg ctcgtcaacg tcggcatttt cctgggggcc
    48601 ggcatcctgc agccgctggc cggtgctctg ctcgacgcgg tcaggcctgt gctggggccg
    48661 acgggggcct gggaccgagc gatctggttg ttcgcagcca gcgccgctgc cggcgccttg
    48721 tgcaccctgt tcgtacgaca cgagtatcca gcccccgcgc cagcgcattc acgccgtcag
    48781 accggggcgg cagcaacggc ggctcgaccc ggcgcaggcc atggatcagt gtgtcactca
    48841 aagagaggga atccttaatt cccttttttg gaatcatagt gtcattttac ggaatcactt
    48901 aggatgccac gcctgccgcc gatccaagcc atccctggac catgatgacc cccgtcacgc
    48961 cccccacagc catgcccttg acggcttccc actggggggt ctatgaagcc acgggccagg
    49021 gcgaggcgtt gcgactgcag ggattcgcac gcgacccctc gccctcgccg atcggcctgt
    49081 cgatgctcga cgccgcgcgc ggtccggtgc gcgtgcgtcg acctgcggta cgtcgcagct
    49141 ggtggcaggc catgcgcggc gatgcaccgc acgcggcacc tcagctgcgc ggcaccgacc
    49201 cgttcgtcga ggtgggctgg aacgaggcga tcgacctggt ggcgcgtgag ctcgatcgcg
    49261 tgcgcctgcg gcatggcaac gaagcgatct tcggtggctc ctacggctgg tccagcgcag
    49321 ggcgctttca ccacgcgcag agtcaggtgc atcggtttct caacgccatc ggcggctacg
    49381 tccgacacgc cgacacctac agcctgggtg cggcccgggt gctgctgccg cacatcgtgg
    49441 caccgatgga agagctgttc acccagcaca ccagctggcg cgtcatggcg cggcacacca
    49501 ggctgttcat cacctttggt ggcgtgccgg ccaagaacgc gcaggtcagt tcgggcgggg
    49561 ccaccgagca cgcggtcccc accgggctgc gccgcatggc cgctgcgggg gtgcgcttca
    49621 tcaacatcag tccggtgcgc gacgacatcg acaccggcgg cgaggtggag tggatcccca
    49681 ttcgcccggg caccgacacc gccctgctgc tggccatggc gcacacgctg tggacggaaa
    49741 aacgtcacga cgccggcttc ctggccagcc actgcgtcgg ctttgaacgc gttgccgcct
    49801 acctgcgggg cgacaccgac gggcagccca agagcgccga ctgggcggcc cggattaccg
    49861 gcgtgccggc cgagcgcatc gtccgcctgg cacgggaagc ggccgccacg cgcaccatgc
    49921 tcaacatcgc ctggtcgctg caacgctccc accacggcga gcagccttac tgggccctga
    49981 tcaccgtggc cgccatgctg ggccagatcg ggctgcccgg cggcggcttc ggtgtgggct
    50041 atggcagcac caacatgatg ggcaacgaca acgccaagtt ctccggcccg acgctgccgc
    50101 aggggcacaa cgcggtccgc gccttcattc cggtggcgcg catcaccgac atgctggagc
    50161 agcccggtgc cagcttcgac tacaacggcc agcgccacac ctaccccgac attcgcctga
    50221 tctactgggc cggcggcaat ccgttccacc accatcagga cctcaatcgc ctgctcgccg
    50281 cctggcagtc caagccggaa accatcgtcg tccacgagca gtactggacc cccaccgcga
    50341 agctggccga catcgtgctg ccggccacca cggcgatgga gcgcgaagac atcggctatg
    50401 ccacgcgcga gcgctacatg gtggcaatga agcggctcat cgcgccggtg ggcgaggcgc
    50461 gcgacgacta cgccatcttc agcgatctgg ccgaacgcct gggcgtgcgc gaggcgttca
    50521 cggagggacg cgacgcccgc gactggctga gcctgatgta cgaacagtcg cggcagcgcg
    50581 cggcccaggc cggcatcgag ctgcccccgt tcgacgactt ctggacccgg ggcctgcttg
    50641 acctggcaca gcccgaggcc gaagtcgtga tgctcgaccg cttccgcgcc gacccgcaga
    50701 gccacccgct ggccacgccc agcggccgca tcgagctgtt cagcgagcgc atcgccagct
    50761 tcggctacga cgactgtccc ggacacgcct gctggctgga accgctggaa tggctcggca
    50821 gcccagccgc agtccggttt ccgctgcacc tgatcagcga tcaaccccac accaagctgc
    50881 acagccagct cgaccacgcc gcctgcagcc aggccaacaa agtcgacggc cgtgagccac
    50941 tgaccctgtg ccgcatcgac gccgaggccc gcggactcaa ggccggtgac ctggtgcgcg
    51001 tgttcaatgc ccgcggcgcc tgcctggcgg cactgcgcat ctccgacgcg ctgcgtcccg
    51061 gcgtcgctcg catgtcgacc ggggcatggt tcgacccgac aagctggctg gccagcggcc
    51121 ccggcaatgt cgaacgacac ggcaacccca acgtgctgac gccggacatc ggcgcttcgc
    51181 gactgtccca gggctgcatg gcccagacct gtctggtcga agtggagcgc tgcacgtccc
    51241 cggagaccag gccaacgcct tacctgctgc ctgaattcgt cggcaccgca caccctcatg
    51301 agccggcctg cacctgaaac gctggaactc agggcaccac cacctcaccc atcgaaacag
    51361 acgagatgaa taccccagtt tgcgactacc tgatcgtcgg cggcggcacc gccggctgcg
    51421 tgctcgccaa ccggctgtcg gccgacccgt cgtgtcgcgt gatcctgctg gaagccggtg
    51481 gcccggacga cgacaaatgg atccacatcc cggccggcat ccgctacctg ctgcgggaaa
    51541 aaaagcacaa ctggttctac atgaccgagc ccagcccgct gaccaacggc cggtcggtgt
    51601 actggccgcg cggcaaggtg ctgggcggct cgagcagcat caacggcatg gtctacatcc
    51661 gtggccagcg cacggacttc gaccgctggg ccgcatcggg cgccaccggc tgggactggg
    51721 acagcctgtt tccgtacttc ctgaagatcg aaaaccagac ccggggcgcc gacgcctacc
    51781 acgcggtcga cggcccgctg acggtgtcgg accggaccaa tcgcagcgaa gtctggacgc
    51841 gcttcatcgc ggcggcggaa cagctgggca ttccccgcaa cccggacttc aacggtgccg
    51901 accaggaggg cgtcggctac taccaggcca cggtaggcaa cggccgccgc tgcagtgcgg
    51961 ccgtggggta tctgcggccg gtgaagggtc gcgccaacct gagcgtcatc accggggcga
    52021 tggtgcacca catcagcttc gagcagcggc gcgccaccgg tgtgcgctac gaaaaggacg
    52081 gccactggca tgagctgcgc gccgcgcgcg aggtgctcgt ctgcggcggt tcgatcaatt
    52141 caccgcagct gctgatgctg tcgggcatcg ggccggcagc ccaccttcag gagatgggca
    52201 tcacgccacg ggtggacgca ccgcagctgg gcaagaacct gcaggatcac ctgcagttgc
    52261 gcctgaccta ccgactcaac cgtccgatct cgttcaacga ccagttccat tcgctggccg
    52321 gcaaagtcgg catggggctg gaatacctgc tgcgccgcag cggcgccatc gcctacccga
    52381 cggcccaggt cgggctgttc accagaagcc tgcccgagct tgcgagcccc gacatccaat
    52441 accacttctc caactacacc accgacgaaa aaaccggcat gccggaccgc tttccgggca
    52501 tgaccttcag tgtctgtcac ctgcggcccg aaagccgcgg cgagatcctg ctgcgcagtc
    52561 cggacccggg ccaacacccc cggatcctgc ccaactacct ggccacgccg gaagactgcc
    52621 gcgtggccgt cgaggaggtt cgcctcacgc gccggctggc cgccacgcaa ccgctggccg
    52681 acatcgtcgc cgaagagctg gcccccggcc ccgacgtgca gagcgacgaa gcgtgcctgg
    52741 atttcgcccg ctcgaatggc acctccatct accacccggt cggcacctgc cgcatgggca
    52801 gcgacgccga ggcggtggtc gacccggcgc tgtgcgtgcg cggcgtcagt cacctgcggg
    52861 tggtggacgc gtcggtgatg ccgacgctga tttccggcaa cacctacgcc gccaccctgg
    52921 ccatcgccga acgcgcggcc gacctgatcc tgaacacccg cccgtaggcg ccaaccccat
    52981 acccacactg aaggagcaag ccttgaaact cacccccatc ctcaccggcc tgtccgcctg
    53041 cctgatggcc gcggcagcgc acgcccagtc ggtcgacaag taccccgagc ggcccatcaa
    53101 gatcatcatt ccgtaccagg ccggtggcgg cacggatgtg atgacgcgca tcatcgtcga
    53161 gaaggtgcgc gagcgcacca agcagccctt catcatcgaa accaaacccg gaggcaacac
    53221 cgtcatcggc gtcaaggccc tgctggccgc gcccgccgac ggctacacgc tgatgatgac
    53281 cagcagcgcc accttccaca ccgcacccat cattgtcaag aaccctggct acgaccccat
    53341 cgagtcgttc tcgctcatca gctacatcgc ctataccccg gtggtgttcg tcatcaattc
    53401 gtcggtgaag gccaacaacc tgaaggaatt catcgatctg gccaaggccg cgccgggcaa
    53461 gtacgcgctc agcacctacg gacgacagct gggcaccgac ctgttccaga aggttgccgg
    53521 cttcaaactc aactccatcc cgtacaaggg cgtggaagcg gtcaatgccg tggttgctgg
    53581 ccacacggat gcgatgttcg atggcgtctt caccgccctg ccctacctca agcagggcaa
    53641 gacgcgcgca ctggccgtga tgcaggacaa acgcacccgc ttcgcacccg acattcccac
    53701 ggtcaccgag ctgggctaca agtccatgcc cgacatcccg atctggtacg gcctggtggg
    53761 ccccaagggc gtcgaccaga aaatcattga caagctggcc aacgagttca agatcgccat
    53821 ggagtcgccc gaagtgatcg agaagctggc caacctcagc gtcgaatccg tcggctcggg
    53881 cccgcaggcc ttgcgcaagc agatcaccgc cgagtatggc cactaccgca agattgccga
    53941 cgaactgggc atccagcccg aatgacgccg cgccgcgccg atcgacgccc ggccccacgg
    54001 agacacccat gacacacccc gaccgcctga gactgagcct gccgctgctg ctggctgccc
    54061 cgcaactgct gtggtccgtg cccgcacggg cgcaggacaa atggccgtcc gggccgatcc
    54121 gcatggtggt gccgtttccg cccggcggtt catcggacat gctgggtcgc ctgatggcag
    54181 acaagctcag cgcgctgctg aagtccaaca tcttcgtcga gaacaagccc ggtggcacca
    54241 cgcagatcgg caccgagctg gtggccaacg cggcccccga cggccacacc ctgctgatgg
    54301 cctctgccac cgggttcacg gccctgccca acctgcgcaa gctgaactac ggcatcgaca
    54361 gcttcgagat cgccggcggc gtggccgact acgtcgccgt catggcggtg cgcaagacct
    54421 tgccgatccg caccgtcaag gagttcatcg actacgccaa gcagcatccc ggcaagctgt
    54481 cgttcggctc ggccggtgag gcgtcggcgg gccacatcta cggcctgaca ctggcgcgcg
    54541 acaccggcat tcaggtgctg catgtgccat ttcgcggttc ggcggcggcc gtcaacgccc
    54601 tggtggccgg cgacgtcgac ttcatcatcg acggcgccgt cacccccatg gtcaaagccg
    54661 atcgcgtgca gccgctggcg gtgctgtatc ggcgtcgcca ccccgagctg cccggcgtgc
    54721 cgacgctgga ggaagccggg ttcaccatca gctccacccg cggttcgggc tggggcgtgc
    54781 tggcgcccaa gggcacaccg gcggccatca tgtcgcggtt gtccgaggcg atccgtcagg
    54841 tgctgcagca gcaggacgtg aaggacgcgc tggcacgggc caactcggtg ccttcgtggc
    54901 aggcgccgga cgagtatcga cgcagcctgg atgccgaccg caagctgtac gccgagctgc
    54961 tgccggcgat cggcattcgc ggcaactgag gcaccggtca tggtcgacgt ctccaacccg
    55021 gcggcgtggg ttgcaccacg ccagagcgtc cgcgacatcc tgcacccgcg cagcgtggcc
    55081 atcgtggggg cttccgacag cgaagacaaa tggggcgggc gcctgctgcg ctacatgctg
    55141 cggcatcgcc acccgggcgc gctctacccc atcaacgccc gtgccaccga gctcatgggc
    55201 ctgccggcct acccttcgct gcgcgactgc cccggcccgg tggacctggc gattctgctg
    55261 gtgccgcgcg cgcatgtccg cgcggccatc gaggactgcg tggccaaagg tgtgggctgc
    55321 gcgctgtgca tcacggccgg gtttgccgag accggcgcgg ccggccgcgc cgacgaacag
    55381 gcgctggtcg acctggcgcg cgccggcggt gtgcgcctga tcggccccaa ctgcatgggc
    55441 ctgatgaaca cccaccacgg gctgtgtgcc accaccggcg tggtgatggg caccctcgac
    55501 aggctgccgg tcggcggcat cggcatggcc agccagagtg gtgcgctgat gggcgcgatg
    55561 atttcgcgcg gggtcgacat cggggctggg ttctcgacca cggtctcggt cggcaaccag
    55621 gccgacctcg acctgaacga cttcttcgaa tacctgatcg acgatccgaa gaccgaggtc
    55681 atcacgctct acatggaagg cgtgaaggac gcgccgcgct tcaccgccct tctggagcgc
    55741 gccgccgccg cgggcaaacc ggtgtgcatc gccaagtcgg ggcgcagcgc ggccggcgcc
    55801 aaggccgccg cctcgcacac cgccagcctg gccggcgcct ggcccgcctt cgaggcgacc
    55861 tgccgccgct ggggtgtcta cctgttcgag accatctacg atctgctgca gggcgcgctg
    55921 ctgctgcaac gcggcaagcg ggcggcgtcg gatcaggtgg ccgttttttc gggctccggc
    55981 gggggcggtg cgctgctgac cgacggtctg gaagcgcacg gcctcgcccc ggccgagctg
    56041 tcggacgcca cccagcaggc cgtcgccgcc gtgttgccgt ccacgcaccg ccagttgccg
    56101 ttcgacttcg gcatgctcaa ccatgcggca gcgcccgatc cgcaggtggc gggcggcgca
    56161 atcggcatcg ccctggaccg cgccctgcac gaccccgcgg tcggcgccgg catcctgctg
    56221 ctgaccaccc agcccggcca ggagctggtg gtgcaggcgg ccatcgccgc cgccgaccgc
    56281 tcctccaagc ccctgctgtt cgtccagggc gccggcaacc acggcgacgt ggcgcgccag
    56341 atgttgcgcg cggccggcca cggctacttc gacagcccgc acgacgcgct gaaggtgctg
    56401 tcggtgctgg cgcggcgcaa gccgccaccg cccagcgcgc caccgcagcc cggcgtcagc
    56461 gtcgccgccg atctgccggc cggtttcctg accgaacctc aggcccgccg cctgctggaa
    56521 ggggcgggca tcccgaccac acgctggcgg ttcgccgccg gcgccgacgc cgcggcccag
    56581 gccgcgcgcg aacttggcgg cagcgtggcc atcaaggcgg tcagcgcgca catcgtgcac
    56641 aagagcgaca tgggcggcgt caagctcggc ctgcagggcg atgccgccgt gcgccaggcc
    56701 tgcgccgaca tcgccgcagc ggcgcgcgcg gccggcgtcg atacccttga cggctacctg
    56761 gtcaccgaga tgtggcgcgc cgacaccgag ttgatcctgg gtgtgcagcg cgaccccgac
    56821 ttcggcccgc tggtgatggt gggtgccggg ggcgtgctgg tcgagctgat gaaggacgtg
    56881 cagctggcgc ccgctccgct gtcgcacgcc acggcgctgg cgatgctgcg acagctgcgt
    56941 tgcctgccgc tgctgagcgg ctaccgcggc cgcacgccgg ccgacctgga cgccatcgcc
    57001 gacgtcatgg tgcgcctggg cgcgctggcg gccgacccca gcgcccggct gcgcgaactc
    57061 gacatcaacc cgctgttcat cgccgggccg cgcatcgcgg cggccgacgc gcgcgccacg
    57121 ctggcctgat gcacctacca ccgaacagct tgcacctgaa gcaaccatga ccgccgtacg
    57181 ctacgcctcc gagggcggca tcgcccgcat caccatccac cggcccgaag cccgcaacgc
    57241 catcaacgac gaggtggtgc agggcatgca ggccgcatgg caacgcttcg cccaggacgc
    57301 cgacgaccgc gtggcggtgc tgcacggcgc gggcgagcag gccttcacgt cgggcgccga
    57361 cctgaaagcg ctgccgcgcg acatctggct ggccatgccc aacctgtcgg tgccgtgcga
    57421 caagccgatc atcgccgccg tcagcggcct ggccgtcggt gccggcgcca ccatggtgat
    57481 gctggccgac atggcggtgg ccgacgagga cgcccgcttc gtctacccgg aggcccggat
    57541 cggcgccttt gccggtgtga tggctggttt tccgccccgc atgcaataca aggccggcct
    57601 ggaatggctg ctgaccggcg accccatgac ggcccagcgc gcctacgaga tcggcctggt
    57661 caaccgcctg gcgccgcacg gtcaggcgct ggaggtggcc atgcaggtgg ccagccggat
    57721 cgccgccaac gcaccgctgg tggtgcaggc catgaagtcg atcgcgcggc gcacgctgcc
    57781 ggcttcgccg accgagctgt attacccgca gcaccgcatg ctgcaaggca tcgccgccag
    57841 cgccgacctg cgcgaaggcg tggcctcgtt caaggaaaag cgcgccccgc gcttcagcgg
    57901 ccgctgatcc ccgcactcaa ccccaaggag cattcaccat gaccctcacc cgtcgatccc
    57961 tgcacctgct ggcgctggcc gcgctgtccg gtgccgtcgc gcccgcgctg gcccagacgt
    58021 ccaagccctg gccggacaaa cccatccgct tcgtcacacc gtacccgccc ggcggctcgt
    58081 cggacgtcat cacgcgcttc atcggcgatg gcgtctccaa gatccttggc cagccggtga
    58141 tcgtcgagaa caagcccggc gccgccgcca ccatgggcac cgagttcgcg tcgcgccagg
    58201 cgcccgatgg ctacaccttc ctggtggcgc ccaccgccgc cctggcgctg gcgccctacc
    58261 tgatgaagaa cgtcaagtac accttcgaga gcttcgaccc ggtggccaag atcgcttcct
    58321 cctacggtct ggtgacggcc cgcaaggacg cgccgtttac cgattacaag ggcatgctgg
    58381 cctacgccaa ggccaacccc ggcaagctca cgttcgccac caacggcgtg ggctccatcg
    58441 tgcacatgac cgcggtgctg ctgcacaagc acgccggcgt gcagctcacc cacatcccgt
    58501 acaagggggc gggcgagtcg gtgatcgacc tgctggccgg ccgcatcgac ctcatgtacg
    58561 acccggccac ggcaccgcgc gtgaagtccg gcgacctcaa ggggctgggc accaccagcg
    58621 gcatccgcaa ccccgaactg cccaacctgc cgacgctcaa ggagcaaggc tttggcgacg
    58681 gtggctcgtt cagctggttt gccatgttcg caccgaaggg cacgccggcg ccggtggtca
    58741 acaagatggc cgacgccgtg cgacaggtgc tggaagcgcc ggaagtcaag gccaagctgc
    58801 agctgtcggc gctgtacccc aactacgagg acccggcgac ctttgccaag tcggtcagga
    58861 acgacgcgga aacgctgcgc aacgtgatcc agcaggaagg catcaagctg gagtgacctt
    58921 caaagcggcc gctgaccgtt ggtcagcttc gcaaagctgg gacgaacgca gggatgggcg
    58981 gcctggtgga tcgtcagctg gtgagtcagg ctcagctgcg ccgccagacc ctgcagggat
    59041 gcgcctgcag acgcacaaca gccgcagcca gccgccgcgt tcagactcag cccagcgcca
    59101 gccgcgccgc ccgcaccagg gcccgcgcgt cgacgccgaa gaagcgccgc agcgccgcac
    59161 gggtgtcgct gcgcccaaag ccgtcggtgc ccagcgtgag gtagctgcgc ccttcgggca
    59221 cgaacgcccg cacgctctcc ggcacggcgc acacgtagtc ggtggcggcg atgacgggcc
    59281 cctgggtgtc actcagctgc tgggtgaccc agggcacgcc gggcgtgtcc gcgccggcca
    59341 gacggcgctg ctggcaggcc aggccatcgc gcgccagttc gctccagctg gtgacgctga
    59401 gcacggtggc ggcaatgccc tgacgcacca gcagctcggc ggccaggatc acttcagtca
    59461 ggatggcgcc ggagccgagc aaggtcaccg ttggggccgt ttctggcagt tcgcgtccgt
    59521 ccggcgtgcg ccgatagcta ccaaatagat agcaaccacg caacacccct ggatgggcat
    59581 ccgccggcag cgcgggctgg gcgtagttct cgttcatgag cgtgacgtaa tagaacacgt
    59641 ccttctgctc caccatcatc tcgcgcatgc cggcgtccac gatgaccgcc atctcgcccg
    59701 caaaggccgg gtcgtaggcc ttgcagttgg ggatggtggc ggccaccagg tggctggtgc
    59761 cgtcctggtg ctgcaggccc tcgccgccca gcgtggtgcg gcccgaggtg gcgcccagca
    59821 ggaagccgcg cgcgcgctgg tcggcggcgg cccagatggc gtcgcccaca cgctgaaaac
    59881 cgaacatgga gtagtagata tagaacggca gcatggccag gccgtgcacg ctgtagctnn
    59941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    60001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc agcatggcca ggccgtgcac
    60061 gctgtagctg gtggccgcgg ccgtccagct ggccagcgca ccggcttcgc tgatgccttc
    60121 ttccagaatc tggccgtcgc gcgcctcgcg gtaggacagg atggagccga tgtcctccgg
    60181 cgcgtagcgc tggccgacgc tgctgtagat gcccacctgt ttgaacaggt tggccatgcc
    60241 gaaggtgcgc gcctcgtcgg cgacgatggg cacgatgcgc ggcccgagct gcccatcctt
    60301 gagcaggtta cccagcatgc gcacgaaggc catggtggtg ctcatgtcct tgccgtcggc
    60361 ggccacggca aactgcgcgt agctggtcag ggccggcacg gccaccgcct cgcacagcgt
    60421 ctgacgggcc ggcaaccacc cgcccagggc gcgccggcgc tgcagcaggt actgcagctc
    60481 ggcgctgtcg accgccggct tgaggaactc caggttggcg gcctgcgcgt cggtcagcgg
    60541 caggttgaag cggttgcgaa acgcgatcag ctcctgctca tcgagcttct tctggctgtg
    60601 cgtggtcatc ttgccctgtc cggccgcgcc catgccgtag cccttcttgg tgtgcgccag
    60661 gatcacggtg ggctggccgc ggtgggcatg ggcggccgca taggcggcat ggatcttcac
    60721 caggtcgtgg ccgccgcgct tgaggcggtc gatctgctcg tcggtcatgc cctgggccag
    60781 ttgctgcagc tcggggctct ggccgaagaa gttgtcgcgg ttgaagcgcc cgtccttggc
    60841 ggcgaaggtc tgcatctggc cgtccacggt ctcgcccagg gtgcgcacca gcgtgcccgt
    60901 gaggtcgcgc gccaacaggc catcccagtc gctgccccaa agcagcttga tgacgttcca
    60961 gccgctgccg gtgaacaggc gctccaattc atcgacgatg cggccgttgc cgcgcaccgg
    61021 gccatcgagc cgctgcaggt tgcagttgac cacccagacc aggttgtcca ggccctcgcg
    61081 cgcggcgagc gtgagggcgc tggtgctctc gggctcgtcc atctcgccgt cgccgaacac
    61141 gccccacacc ttgcgaccgg cacagtccag cagaccgcgg tcggtcaggt agtgcatgaa
    61201 gcgggcgtgg tagatgctgc tgatcgggcc gatccccatc gagccggtgg gaaactgcca
    61261 gaagtccggc atcaggtagg ggtgcggata gctgcacagg ccgcgcgcgc cggcgccggg
    61321 cgccgtcagc tcctggcgga aatgcagcaa atcctgctcg gacagccgcc cttcgagaaa
    61381 ggcgcgggcg tagacgccgg gggcgctgtg cggctggaag aacaccagat cgccccggtg
    61441 ctggcccggc gccagaccct cgcgggcgcg gaagaagtgg ttgaagccga cctcgaacag
    61501 atcggccgcg ctggcgtagc tggcgatgtg gccgcccagc tccccatagg cctggttggc
    61561 gcgcaccacc atggccagcg cgttccagcg gatcagcgag ccgagccgct cctcgatcgc
    61621 cagatcgccc ggaaacaccg gctggtcctg cgccgcgatg gtgttgagcg ccggcgtgtt
    61681 gagctcgggc cgccagccca gctggcgcgc gcgcgccagg cgcgccagct cgtccagcat
    61741 gaaacacgcg cgcgccgggc cctgagtggc gatcagcgcc tcgaacgcgg cgcgccattc
    61801 ggcagtttcc tgcgggtcgg catcggcggg cagggacgcg gaagacgcca gggcctggga
    61861 atcgatcgga gcatgcatgg tggcgactca gtggactgca cagaaaaacc atcgcggcgc
    61921 gccggcacac accgtcgccg accgcgccat cgtcggcatg atcccgcagc gcctgtcggt
    61981 ggtcaatgcg ggccggcggc accggctcag aacacgctgc tcaggctgcg cgccccgcca
    62041 cgggcggcgc cctccggcgc cagcgcgtgg tccagcgcct gaccgatgtc ggccaggatg
    62101 tcctccacgt cctccaggcc aatggacagc cgtaccagcc cttccggaat gccatgcgcg
    62161 gcgcgttctt cgggggtgta ggtcgagtgc gtcatgctgg ccgggtgctg cgccagggtc
    62221 tcggcatcgc ccaggctgac ggctcgcgtc accaggcgca gggcgtccat gaagcgcaca
    62281 cctgcctgca ggccgccgtg cagctcgaac gcgatcatgc cgcccatctg gcgcatctgg
    62341 cgctgcgcca gcgcgtgctg cgcaaagctt ggcagcccag ggtagtgcac cacggccgcc
    62401 gccggatgtg cggcgatccg ctcggccacc tgctgcgcgt tctgacagtg gcgatccatg
    62461 cgcagtgcca gggtcttgag accgcgcatc accaggtggg cgtcatgggc cgacatcacg
    62521 gcaccggtca tgtccttcag gccgtacagg cgcacgcgct cggccagttc ggcgtcggca
    62581 aacacggccg cgccggccat caggtcaccg tggccgccca ggtacttggt gaccgaatgc
    62641 aggctgacgt cggcccccag caccagcggc tgctgcaggt agggcgagca gtaggtgttg
    62701 tccaccacca cccgcgctcc gcgggcatgc gacagacgcg agatggcggc gatgtcggcc
    62761 agtcgcatgt tggggttggc gggcgattcg atgtagacca cgcgtgtgcg ctcggtgagc
    62821 gccgccgcca cctgctcggg atcggtcatg tcgacgtggc gcaccgtgac gccgaagtgc
    62881 ccgatgccgt ggtgcaggaa cgaaaaggtg cagccgtaca gcgtcaggtc ggcaagcacc
    62941 tcgtcacccg gcgtcagcat ggaccacagc gtggccgtga tggcgcccat gccggaggcg
    63001 aacaccaccg cacccgcgcc ctgctccagg ctggccagac gcgcctccag cagcgccagc
    63061 gtggggttgg cgatgcggct gtagatgtag cctttctcct cgccggtgaa gcagcgcgcg
    63121 ccgtagcccg tgtcgggaaa ggcgtaggtg gccgaggtgt ggatgggcgg caccagcgcg
    63181 cccagatgct cggccgggtc gtagccgaag tgaatggcgc gggtgctgaa cccacccgct
    63241 cgatcaaggg acctggtcat gaaagaatct cctgcagcgt caaatcagaa gcgtattttt
    63301 tccttttacg acgggcaaga attggctatc ttgatgcttt tatggcagca cgatagcagt
    63361 ttgttttttt ttcattgttg tttggggcaa atcatgtctt caccctccgg ctccttcgat
    63421 catgtggatc gcgccctgct cgaagtgctg cagcgcgacg gccgcgccac ggtgggccaa
    63481 ctggccgaac gggtctcgct gtcgccctcg ccctgctggc ggcgcgtgcg ccagctggaa
    63541 gaaagcggcg tcatcagcgg ctaccacgcc cacctcgacc gtcggcgcat cggctatggc
    63601 gtgctgggct tcgtccagtt gaacctgcac gatcacacgt ccagcatctg cgccgcgttc
    63661 gagcgtgagg tgctggcgct gccgcaggtg ctggcctgcc acaacctgtc cggccgccac
    63721 gactaccagc tcgaagtggt ggcgcccgat ctggagtact tcgccgagtt cgtgcgcagc
    63781 cgcatccgca gcctgcctgg cgtgcgcgag atttccacca gcttctcgct caaggagatc
    63841 aaacgcacca ttgccctgcc gctcgaccac tgagcccagg ctgaatccca ccctgcatac
    63901 actgcggcct ttcaaggcgt gcaaggacca caaacaccat ggcggacttc gacttcgacc
    63961 ttttcgtcat cggcggcggc tcgggcggcg tgcgggcggc gcgcttcgcg gcgcagcgcg
    64021 gcgctcgcgt ggcgctggcc gaaagcggtc gcatgggcgg cacctgcgtc aacgtgggct
    64081 gcatccccaa gaagctctac agctatggcg cgcactacgc cgaggccttc gaggaggcgc
    64141 gcggctacgg ctggcagctg cccggcgcgc cacggctgga ctgggacgcg ctgaaggccc
    64201 gccgagcgca ggaaatctcg cgtctgaacg gcatctatga aggtttgatg accggcgccg
    64261 gcgtgcggcg tctgcaaggg catgggcggc tgctcgacgc ccacacggtc gacgtgacgg
    64321 cgccggacgg cggccgcagc cgccaccgcg cgcgccacat cctgctggcc accggcggca
    64381 cgccggtgcg acccgagctg cccgggcacg agctgatcgt cacttcggac gacatgttcg
    64441 acctgccgcg gtttccgcgc cacctggtgg tggtgggcgg aggctacatc ggctgcgaga
    64501 tggccagcat cttccacggt ctgggcgcgc aggtcaccct gctgtaccgg ggcgagcaga
    64561 tactgcgcgg cttcgacgac gaggtgcgcg acttcaccgc ggccgagctg cgcaaggccg
    64621 gcatcgatct gcgcgtgcgc accgaggtca cgcgcatcga ggccgcgccc gacggcacgc
    64681 gccgcctgac cctgaacgac ggcggcgtgc tgcaggccga cgtggtgctc tatgccgtcg
    64741 gccgccggcc caacgtggcg ggcctcggcc tcgaagccgt gggcgtgcgg atgaccgcca
    64801 gcggcgccat tgcggtggac gagcacttcg ccaccgcggt gcccggcctg tacgccctgg
    64861 gcgacgtgcg cggcgggctg gagctgaccc cggtggcgct ggccgaggcg atggccctgg
    64921 tcgaccacct gttcggcccc ctgcccggcc aggccaggcg caccatggac tacgagcaca
    64981 tcccgaccgc cgtgttcacc catccgccca tcggcaccgt gggcctgacc gaagcccagg
    65041 cgcgcgcgcg ggtcggcacc atccgcgtct atcgcagcga cttccggccg ctcaagcaca
    65101 ccctgtcggg gcgcgatgag cgctgtctgg tcaagctgat cgtcgatgac gccagcgacc
    65161 gcgtgctggg cctgcacatg gtcggcgccg acgccggcga gatcgtgcag ggctttgccg
    65221 tggccctgaa atgcggcgcc accaaggcgc aattcgacgc caccctgggc atccacccca
    65281 ccagcgcgga agaattcgtg acgctgcggg acgtgacgcg cacctgagca gtgcgcatga
    65341 acccctcgtg tccatcttct gaaccggcca gcgctcagcg tcgaccggcc aggcgccggc
    65401 catcgcgcag gttcagcgcc agcaacgtga tgttggtggc cccggccacc aactccagcc
    65461 cctgcagcgc atagaagcca gcgtccagcc ggcccgcgct ggcccatcgc gccagcagca
    65521 aggcactggg cagcagcacc agcaggccgt tgaccgccac catcttcatg cgccgctgct
    65581 tggcttcgac cagccggccg cgccgcgagc gcgccaacga gaagccactg ccgccggttg
    65641 cggccatcgc cgggatcagc acccacatgc cggccaccac catgttcttc acgaacacca
    65701 cgctggcctg gtcgagcagc agctcggcga cgaccgtcga cagccagaac gtgacaatgc
    65761 acagcagcgc caccagaccg aacacaccat gcagtttcgc tttcatgtcg catcctccaa
    65821 gtcggcggtg gcgctgccca gcacgtgctc gatgtgctgc agcacctcgg tggcttgacg
    65881 cgcctgcgtt gccgaaatct gtctggccag ctgcgcgacc tgatcgcgcc acagcgcatc
    65941 ggcctgctcg tagcacttgc gcccgtgtgg cgtaaggcaa tagagcggcg agcgccggtg
    66001 cgccgggttg ggatcgacct gcaccaagcc cagcccctgc aacacgccca gttgcttgag
    66061 cgcgccctgg cgagacacgc ccatcgccgc acccacctgg ggcgcgctca gaggcgagtc
    66121 cgccagcgcc agcgcaccca gcatctgcca gcgcgcgctg ctcagcccca gcgtcgccac
    66181 ccgtcgatca ccccagtgca gcaaggcgcc attcaggcga aacaccgcca gcgcaagtgc
    66241 gctgatcgca tcgatctgcg gcagggcatt catggttcag gcttcggccg tgatattgac
    66301 aaccagttta cccatttggg aaaccggttg tcaatgcgtg acggagaccg aagccgcggc
    66361 gcgccggctg acgcgcccca gcatcagcca gaccagcagc gccaccacga tcagcgcgcc
    66421 gccgccggcc tgccagaagg ccaccggcga atgctgcgcg gccagcggcc cgacgccgtg
    66481 gtacgccagc aggtagccgc cggccaggcc cacgccccac agcagcacac cgtagaccac
    66541 gaacggcgcc agcgtgacgc ggtagcagcg cagcaggaac atggccaccg tctgcaaggc
    66601 gtcggccgca tgcagcagcg ccacccagcc cagcagccgc gccgccagca gcaccacccc
    66661 ggcctgcgcc gaatagaccg ccgcgatcca cggttttgca acaaaaacca tagctgccag
    66721 cgcaatcccc aagccgcctg caagccagaa tgactgcatg acgacggcac gcgcccgggc
    66781 ctcgtcgccg gccccgcgcc accagcccac gcgggcgctg gaggccaccg ccagcgacag
    66841 cggcgccatg tacatcagcg cggcgacgtt ggcggcgatc tggtgcgccg ccgtcgccgt
    66901 ggtgcccagc cgcgccacga acagcgccat cagggtgaag gacgtgatct ccaccagcat
    66961 ggtcagcgcc gccggcacgc ccaggcgcgc aaaggccgcc agcgtcgggc catgcggcgg
    67021 ttcggggcgc cgccacagtt gcagcggctc atacagcggc tgggtgcgca gcagccacag
    67081 cgccagcccc agcaacaggc catgaacggc gaaggtggcc caggcgcagc ccaccacgcc
    67141 cagggccggc acaccggcgc cgccaaaggt cagccacacc gacagcggca gcttgacggc
    67201 cagcgccagc acctgcaacc agctcaccag ctgcggccgg cccagcgcct ggttgagcgt
    67261 gccgaaggca cgaaacagca gcgccgccgg caggctccag cccagcacgg ccaggtagtc
    67321 gaccaccacc ggctgcaacc cggccggcac gtcggtccag cgcaacagcg gctccggttg
    67381 cagcagcacc gccagaccca gcagactggc cgcggcccag atgtacagcg cctgccgcac
    67441 cgagccgccg acctcgccca gccggcgcgc gccgcgcagc tcggcccaga tcggcagcag
    67501 cgccgtgaac aggcccatca gcgacacatg cacggtgacg aacacggccg cgccgatcga
    67561 cagcgtggcc agcgacaggt cggaatgccg ccccgccacg atggtgtcga ccaccgcaaa
    67621 acccatcacc gcgacctggc cgacgaacac cgcccaggcg tcgccggcga tcacccgcag
    67681 atcgcttcgg tcggcacgcg cggcaggctg gcaggagtcg ctggcggtcg tcacggcgcg
    67741 cattgtggca gtgaccgtgc gccgcagccg gacggcgagc cacccgggcg acagggcggg
    67801 cgcgctgcgg cgccaggcca tcgccggcgc acccaggcgc tacgatggtg cctcgcgagg
    67861 agagcacccc atgaccgacc tgagcaagat cacctgcatc gaagacctgc gcgtgctggc
    67921 caagtgccgc gtgccgcgca tgttctacga ctacgccgat tccggcagct ggaccgaagg
    67981 cacctaccgc tccaaccagg ccgactttgc acccatccag ctgcgccagc gcgtggccat
    68041 caacatggaa gggcgcagca ccgccaccac catgatcggc gagccggtgg ccatgccggt
    68101 ggccctggcc cccaccggcc tgaccggcat gcagcacgcc gacggcgaga tcctggcggc
    68161 gcgcgcggcg cgggcgtttg gcgtgccctt cacgctgtcg accatgagca tctgctcgat
    68221 cgaggacgtg gctcagcacg ccggtccggg cttctggttt cagctctacg tgatgcgcga
    68281 ccgcgacttc atcgagcgcc tgatcgaccg cgccaaggcc gccggctgcg gcgcgctggt
    68341 gatcacgctg gacttgcaga tcctcggcca gcgccacaag gacatcaaga acggcctgtc
    68401 caccccgccc aagccgaccc tggccaacct gatcaacctg gccaccaagc cgcgctggat
    68461 catgggcatg ctgggcacgc cgcggcgcaa gttcggcaac atccacggcc atgtgaaagg
    68521 cgtgtcggac atgagcaacc tgggcgcctg gacggccgac cagttcgacc cgcgcctgaa
    68581 ctggggcgac gtcgaatgga tcaagaagcg ctggggcggc aagatcatca tcaagggcat
    68641 catggagccg gaagatgcgc gcctggcggt cgactcgggc gccgacgcgc tgatcgtcag
    68701 caaccatggc ggccgccagc tcgacggcgc gccctcggcc atcgccgccc tgcccaccat
    68761 cgtggacgcc gtgggcacgc agatcgaggt gcacatggac agcggcatcc gcagcggcca
    68821 ggacgtgctc aaggcctggg cgctgggcgc gcgcggcacc tacatcggcc gcgccttcct
    68881 gtggggcctg ggtgcggccg gcgaggcggg cgtcgccaag gcgctgcaga tcatccacaa
    68941 ggagctggac ctgaccatgg ccttctgcgg tcacacgcgc atcgaaagcg tggatcggcg
    69001 cattctgcgg cccggcacct ttcccacccc ctgaacccga ggcgcccatg ctgcaccccc
    69061 aagcccaggc cctgatcgat ctgctggtgg cccatggcct gccacccacc cacacgctga
    69121 cgccggccga agcgcgcgcc ttctaccgcg agcgccgcgc gctgacgcag cccgccccgc
    69181 ccgacgtggc cgagctgcgc gagctgcgcc tgccgggccc ggcgggcgag ctggcggcgc
    69241 gcgcctaccg gccgctgggc gccaggcccg aggccgtgct gccggtgctg gtgtactttc
    69301 acggcggcgg ctgggtgatc ggcgatctgg acacgcacga cacgctgtgt cgcgagctgg
    69361 ccaacgccag cggctgcgcc gtggtgtcgg tggactaccg gctggcaccg gagcacccgt
    69421 ttcccgccgc cttcgacgac gcgctggcgg ccacgcgctg ggtggcgcgc cacgccggcg
    69481 agctgggcgc cgacccgacc cgcctggcgg tgggcggcga cagtgccggc ggcaacctgg
    69541 cggccgccgt ggcgctggcc gcgcgcgacg acagcgacgc cggcggcccg ctgccgatcg
    69601 cctttcagct gctgatctac ccggccaccg acatgcgccg tggccatggc tcgcaccagc
    69661 gcaacggcca gggctacatc ctcacgcgcg agaccatgga ctacttcatc ggccactacc
    69721 tgccgcccgg cacctcgccc accgactggc gcggctcgcc gctgctggcg gccagccacg
    69781 tcggcctgcc gccggcgctg gtgctgacgg ccggcttcga cccgctggtg gatgaagggc
    69841 tggactacgc gcgcgccctg accgccggtg gcagccgcgc cagctacgtg tgcttcggcc
    69901 gccagatcca cggcttcatc accatgggcc gggtgctcga cgaagccaac agcgccgtcg
    69961 ccctgtgcgc caccgagctg cgccgcgcgc tgacgccgtg acgcccgccg cgccgccggc
    70021 tcacagccag cgccgcacgc gcgcctggta gtcgatgtag tcctgcccga agcgctcgcg
    70081 caggatgcgc tcctcggggc cgatctgcag ccggttgagc agcagcaccg acagcggcag
    70141 ccccagcagc gccgccgcgc tgcccagcca cacggcccag ccggccagca ccaccagcat
    70201 gcccaggtac atggggttgc gcgtccagcg gtacaccccg cgggtcacca cggtgcgcgc
    70261 gcgctgcggc gccagcgggt tgaaggtggt gcgcgcgcgg cgaaattcga gcagcccggc
    70321 cagcgccacc agcgcgccga tcagcgccag cccgagtgcc agcgcccaca cccaccaggc
    70381 gccaccggcc ggccacagcc gcgcggctgg cacggcgccg gccagccagg ccatcacggc
    70441 cgcgcaggcc aggtcgatca ccggcggcgg gatccggtgt tcgtaccagc gcctcatcgc
    70501 ggcccttcca aactattgaa aacagagcac ccggcgcacg tcctacttgc gcaacagccc
    70561 tttcagggca gtctgcaggc ggcgtgccgt ggcgtcgtcg tgcgggccgg ccagcacggc
    70621 ggcggtgtcc tgaccccagg tctgggccgg cgacgggtcg ttgcgccgcg tcagcaactg
    70681 cagttgcagc gcgcgccgcg cctgcagatg ttcggccggg gtgggcacct cggccgccat
    70741 ttccagccgc agcagggctt cggccgccgg cgcgccggcg ccgccgccga tggcctgcgt
    70801 ccaggcgttg cgcagcgccg gcgtgacgcc gcggcccagc tcctgcacgc cgggcacctg
    70861 gtcgggctgg cgcgcctgcc aggcgcccag cagctgggtc agcgcctcgc catgggcctg
    70921 cgcggccagc ttgcgcagct gcgcctgggc ctgctcgacg gcgtcgcgct gggcgcggaa
    70981 ggcggcgtcg cccagacgcg ggccacgcgc ctcgtccgag cggcgcgggc cgcgagcgtc
    71041 gtcgaagcgg cgcgcaccgc gctcgcccgc acggccggcc gcaccttcgc gccggtcacc
    71101 cgcgcgcccg tcgcgaccag cggggcggcg ctcgcccggg cggccggccg gcgccggctc
    71161 ggccttgcgg gcaccggggc ggtcgtcgcc gcgcatcgcc accaggggct tggcgggctt
    71221 gggcgcgggg gccggcgcgg cgccctcggc gggggcgtcc ggggcgtcgg tgggcacctc
    71281 ggttgcttct atttcgatag cttctggcgc gccatcggcg ggcgctgcgg ccggtttttc
    71341 ttcagatccc ggggcaggct gggcacgcgt ggcggcttcc agctgggcca tggcggcgcg
    71401 gatgcgctgc gcgtcgccgc tggcgttggc cgcctccagc gccttggcgg cttcgagcac
    71461 ggcgcggtcg tgcgcgctca tcgccgcctg cgccttttcg cgctcggcgc tcttgcggtt
    71521 gaaggcctcg tcgatcggct tgcggaaggc gtcccagagc ttctgctcgt gcttgcgatc
    71581 cagcggcacg gcctgggcct cggcctgcca gcgctgctgc agcgcgcgca cggcgtcgat
    71641 gcgcagctgc ggcgcggcgc ccagcgcttc ggcctcctgg atcagcgcct ggcggcgcgc
    71701 ggtgctgtgc ttctgcatgt tttcgagcgg cagcgcggcg gcgtggatgg cttccttcca
    71761 gcgcggctgc agttcggcga actgcttttc cgacaggtgg ccggcgttgc gccagcgctc
    71821 ggcgaactgg tggatctgac ggttgacggc cttccagtcg ccgccctcgc cggcgtcggc
    71881 gtggctggcg gcccaggcgc gcacttcgtc gatcagtgcc tcgcgctgcg ccttgtgctc
    71941 ggccgcctgg gcgcgcacgc cggcgatcca ctcgtcgacg aacgcatggg cggtgctgac
    72001 ggcgcggtcg aagcgcttcc acagggcgtg gttgggcggc ccgccctggt cggcggcttt
    72061 ccattcgtcg cgcagcttgc ggatcgcctc ctgcagcttg cgaccgccca tggcgggcac
    72121 ccactgctcg tcggcggcca gcgtggtggc gggcaacgga cgggcggtga gttcggcatc
    72181 gttttctgcc ccggcgcttg cagggtctgc gccggcagct gcgctttcga tagtatccgg
    72241 ttgaccctcc ggcgccgtct gctcgggcgc cgccgggctg gcggaggctt cgggggcggc
    72301 ggggacggcg gtgggttcgg tggcgctggc ctggccagac tcggcctgcg acggcgccgg
    72361 cgcggcgtcg ggcgcgccct gggccggcgc cttcttgcgg cgcttgacca gcagggcttc
    72421 ggcgcgcgcc accagctgct cgcgcagctt gtcggcgctc cagcgctgcc agccttccag
    72481 ctcgccggcc gacaccagca cggcgtgcac gcgcgcttcc agcgcgtcgt cgatcaggcg
    72541 gccgtgcgtc ttgagcgact ggcgcagcgc ctgggtggcg ctgtgcatgt tcttggtgtg
    72601 gccggcggtc accgcctgct ccagcagcat gacgccggct tcgaccgcct tgtgggcggc
    72661 ggcgcgctgc tcggggtcga tgcgcggccg cgccggtttg tcggcttcgg cggcggcggc
    72721 cggcgcctcg ccgcgggcgg cgcgcagttc gtcggcccac accggcaccg aaggcagcgg
    72781 cgccgcagcg tcggcggccg ccgcctgggc ctgtgccagc gcggcgccga aggcgtccca
    72841 cacggcgctc agctgggtct gtgcggcctc catctgcggc ggaaagcgca gatcgacgct
    72901 gggccactgc gcgtcggcca gcagggcggc ggcctgctgg cgccactgcg cgacgtcggc
    72961 cgccagcccg atctgctgcg cctgcgcgtc ctgccacggc ttggtggaca gcagctcgat
    73021 gcgctgggcc agcaagatgg cggcctcgcg ctgcaccatg acgcgctgct gcaggtcttc
    73081 caccaccttg acgcgctgcg ccagctgggt gcgcaggccg gccagcggct cgcgcgacag
    73141 cggcgcgccg gccttggcgg cgtcgcgctg ccaggccagg gcgtcggcga tgttcaggcg
    73201 cgccgcctgc agcagcgcct gggccttggc cgcccattcg gcggccaccg cttcctggcc
    73261 cttggcgcgg cgcagctcgt cgagccgttc gcgcagcgcg cgggcggcgc ccttgtcctt
    73321 ggccgacaac tccttgaaca cctcctgcat ctgctcgggc gtcggcccgc tggccagcca
    73381 gtcgcgcacg cgctgggcgc gctcgcccga ggtggcggcg gaaaacacgc cgccggtcag
    73441 cgcgtccagc gggtgggcct cggcggccgg ccgggcgggg ctgtccggcg cgggttgcgg
    73501 gtcgttcgat tcgcgtgaga aggggaacat gctgacaggt gctcttcgac aaaaggccga
    73561 gcgcgccgac ctggcgcgcc cgatgggcgt attttcgccg ggaattggcc gctcgccgat
    73621 cgccccaccg atccggccac cgggaaggcg gtcgcagccg gccgggcggc cgcgccgcca
    73681 ccggcctcag cgcgtggcca gcttgagttc ggcgtgcggc gtccaggccg gcgcggcggc
    73741 cggcgcggac cccgaagtgg cggccgacgc ggcgctggcc ggcgccgccg cggccgcgtc
    73801 cgccgccgcc tgcgcggcgc ccagaaacag ccgatcggcc ggcgccccgt gcgccgccag
    73861 gtagtccttc accgccaccg cgcgctgcac cgccagctgg cgcatggcgt cctggccgac
    73921 ttcgatgtgc gccagcagca gcgcctccat ctcggcttgc ggcacgtcgc gcagcaggcc
    73981 gatggcgttg cgcggccggc ccggcacgtc ggcgcggcgg tacaggcgcc gcagcaggcg
    74041 caggcgctgc tcggcgtcca tcgcatctgt tgcatccgac gcggccccag cgcccgccgg
    74101 atcagcgccg gcagctcctg atggcatagc atccggggcc gggctgcggt cctcggccgg
    74161 gttggcggcg gcgctggcgg cgggcgcggc ggtggcttcg gcgcgccgct cggccgccag
    74221 cagggcctgc aggcgctggc gccgccagcc ctcgcgctcg ggcgccagac gggcatggcc
    74281 gaccacggtc agcgtcagct gcgggcgctc ggccagcgcc ttggccacgc ggtcgagctg
    74341 ctggcgcgcc ctggcatcga gccgggcgct gccgggcgcg aaggcgacct gcgacagatc
    74401 ctcgccaccc gcgccgccca gcgcgcgtgc cagcagggtg aagggcgcgg tgatggcctt
    74461 gccgatcagg ttgaggatgg ccttgacgat caccggcccg agcgaaaact gcgggtcgtt
    74521 gagcgagccg ctgatcggca ggtcgaggtc gatcacgccc tggctgtcgg acagcagggc
    74581 ggtggccagg cgcaccggca ggctggccgg cgcgccggcc accggctcgc tgaactgcag
    74641 ctgcttgagc accagctggt tgctggccgt gagctggccg tcgggctgga tgcggtagtt
    74701 cacgtccatg ctcagcttgc cgcgctcgat gccgtggccg gcgtagcgca ccgcgtaggg
    74761 cgacagcggc ggcagctcca gctcgctcac ctggccgcgg atgtccagcg ccagcggcgt
    74821 cgccagcggg ttgagccggc cgctcacccg cagctgggcc gagccttcgg cacggccgct
    74881 caggttcagc tcggccatct gcacctggcc gtccggcgcc accgagctga aggcgcccag
    74941 ggtgccggcc agctcgctca ggtcggccga gtagttgggg cggatgaagc ggtcggcaaa
    75001 atccacccga ccaccttcga gccgcaccgg ccccacccgc acgaccggcg ccagcgccgc
    75061 cggtgcggcg gccaatgggg cgctggcgcc cgcggcgggt gcgccggcag cgatcgaatg
    75121 ggtggtgccg ctgaattccc cggcggccgg catggcgccc ggcggggtgg ctggggcggc
    75181 gccgggcgag ccgcccgcgg cgtgctcgga cttgagcacg tcctgcaggt tcaggcggcc
    75241 gtcgggcagg atcacgacgc gggcgaagaa gtccgacagc agggtctcgc cgatcccgag
    75301 ctgcgccggc tggccgggat ccagccgcag gtcgacgctg cccaggcgca gcagcttcca
    75361 gctcagcagc tcctcgccca ggccgcccga gcgagcgccg gcgcggtcgg cggccaccgg
    75421 cgccagcgcg gcgcgcggcg cctgggcggc ggcggcgggc gtgaccgagg cggccgatgt
    75481 gacgggcgtg gccggtgtgg ccggtggggt ggtgtcgccg gccgccgggg ttgacgccga
    75541 tgccgacgcc accacgctgc ccggccggct gtgggcacgc agctcttcca ggctggcctc
    75601 gccggtcacg cgcacgcgcg ccccctgctc ggtctgcgcc agctcgagct ggcccttgaa
    75661 actggcgtcg gcacgcagca gatcgacgtt gagccactcg ccgaagtacg gctgcagggc
    75721 atgcaggggc aggcgctgca cgtcggccgt gccctgcagc gccggcggcg acagcgtcag
    75781 ctgaccgcgc cagctcagcc gccccggctc gctgcgaccg gcgcgcagcc gcgccgccag
    75841 ctgcagcggc atcggccgcc ggccgtccag ctcgaactgg cgcaggccca gcgtcagctg
    75901 cgtcagctcg gcctgcaccg ggccgtccgc caccgcggcg tcgtgccagc cgagtatgcc
    75961 gtcggccagc gtcagctcat cgagcgtcag cttccaggcc ggcgccgcgg cggtgggcgc
    76021 gggcgcgtcg gccggcagcg gctggcgcag ccagcgctcg accatccagc gcccgtcggc
    76081 ctcgcgcgcc agccgcgcca gcggcgcgcg cagaccgagc cgcgccacgc gcacggcgcg
    76141 cgccggccag tccaggtgca accgctgcag ctcgatcccc tcggccgcca ccaggcgagc
    76201 gtcgtcgggc agcgcactgc gccggcgccc gcgcgccggt gccggcgcgg cgtcggggtc
    76261 ggtcagggcc agctggctca gcgtcacctg ttcggcgctg agcgtgagtt gcgccggcgc
    76321 cccggcctgc tcgggcgcgg cccagtgcag cgccagccgg gccgccagtt gaccgtccag
    76381 gcgcggcttc agcacgccgg ccaggtaggg cgcgcccagg gccagcggca ggccatcgat
    76441 gcgcgcctgc agctcagcct gccggcgcgt ggcctggccc tcgaaggcca gggtcgccgc
    76501 gccctggccg gcgtccggcg caccggtcag ctgggccgtg ccctcccagt gcagcggctg
    76561 gtccggcggc caggccagct cgcgcgcggc cagctccagg tgttgcagct gcaggcgcgc
    76621 ggccggcgcg ccgtgcgcgc tggtcgaggc gtcgctgaaa tccagcgcgc caccttgcag
    76681 cgccagccgc ccgatcgcca gccgccagcc gtcggccgcc cctgcggggc cagccggggc
    76741 gctcccagat gcagggtcgg atgggccttc agagcccgcc ggatgtgcgc cagcagctat
    76801 tgaatcagta gcatccgggg cgccctgggc aagcaggtcg agctggccgt tggcgcgccg
    76861 gtgcagcgcc agccgcggcc cgcgcagctc gaccgagcgc agggccagcc ggcgctccag
    76921 cggtcgcagc tcgtccagtt cgagttcgag cgcgtcgaag gccagccgct cggccccctg
    76981 ggcatcgacc gccttcagcc cgcgcagcgc caggttgccg ctgacgctca gcgtgggggc
    77041 gccggcctgc gcgaacgcgg cgcgcaggtc ggcgtccagc gtggccgcct gcagccgcag
    77101 cggcagcgcc gccggcaggt aggccaggta gggctgcagg tcgaacgact tcagctgcag
    77161 gctggcttcg gtctggcgcg aggcatcgaa cggcagtgcc tgcgcgcgcg agtcgaaggc
    77221 gctgccattg accacgaagg ccagctgcgg ctccaccttg atctggcgcg ccgccggcag
    77281 gctgctcaga aacggcagcg ccagccgcaa ctggcgcacc tggtgctgcc ggccggccgc
    77341 gcggtcgtcg aagtcgatgc gcccgtcggt cagctcgatg ttgtagagcg caaagcgcgg
    77401 cagcgccgcg ggttcgggcg ccgccggccg cgccgccagc cgcgccagca ggtcgtcgat
    77461 gtcgtagcgc ccctcgccca ggtgcgtcag gtgcagcgcg ggcgactcga tgcgcagggc
    77521 atcgatgacg ggcgccaggc gaaacaccga ttgcagctcg gcgtcggccc acaggcgcgc
    77581 cacgctcagc agcggcggcg cgcccggcgc ggcgtcgatg cgcaggtcat ggagcgccag
    77641 ctgcaacgac cagggccgca ccaccaccgc gccgatgtgc accggccgcc ccagctcggc
    77701 actggcgatc ttctcgccct gccacttgag cagcggcggc agcgccagcc aggccagcgc
    77761 ccacagcgcc agcaggccga ccagggcgat ggtcaggcgg cggcgccagc gccggggggg
    77821 ccaccaggcc cgctccggcg gggcgggcga cgcggcgacc gccgcgcggg tgggctcggg
    77881 gggtggggaa gacagcagag aaccagacat cgggcacgga ttgtcgccct cgacctggcg
    77941 ccctccacct gcgacatggc aaccgcctgc aacaggcgcg cgcccatgag aaagcccggc
    78001 gtttcggcct gcggccggcc cgccgggctt tcaggcagcg tgacaaaagc gtcgtggtct
    78061 gccagggtcc gcatcggaaa gcttgctgtt tccggctgcg gcgggaagca agagattgct
    78121 tcctttcaac ggctgacggc gggtccattc gctgtatcgg ctacagccga cggatgcttg
    78181 ccaacccatg cccgggtcac ccgggcgact tcactgtacc cagccggcgc cgccagacca
    78241 agcgcaaata ttcaatccgc cggcccaccc gcattgcccg cgccaatcgg cgcgcgcagc
    78301 ccgccagatg ccgccatcgc ctgctcccac ggacggagca gccaccaggc gcatccgttt
    78361 cttatatcga aattagaatc aaaactccag atttcagggt tgaccaggta ttttgagcac
    78421 tcctagaatc gtctgttccg acaaagtttg tgggaaaccc tgagacgtct gcccgaccgg
    78481 gcagtcccgg tctgacgaca cctcccgtcc cgcgcgcctg ctgtcaccgg cgcacgtcag
    78541 ccgcttcagg acccgaccaa gagactgaca ccaccgcgca ccctcgttgc tcccttgtcc
    78601 tgcgcgcccc gtggcgcgcc ggcctgtgac ctggtgccgt gtgtcgtgaa tcaaggagaa
    78661 ccatgacagc gtccacacgc cttgccgctc tttcaagcgg cctcaggacc ttcgccggtg
    78721 acgtggcgga aggctttttc gaaatcacgc acaacggtct ggccctggtg ggcgtcgtgg
    78781 tggtgctggc cgtggccctg ctcggcgccc accccgaact gcgccagcag ggcgagcagc
    78841 agttgaccga gtggctgacg gcgcgcaagg tcgccgctct gggcatcacg cccgaactgg
    78901 gcgccatcga gcgcgccacc gccgccaacc cgcgcgaact gccgcgcgaa caggccaacc
    78961 tggcgctgtg gatcagcaag aaataccgcg tcgcgcccga gccggtggcg gcgctggtgg
    79021 cggaggccta tgaagccggc caggccgcca agctggaccc gaccctgatc ctggccgtga
    79081 tggcgatcga atcgagcttc aacccctttg cccaaagctc ggtcggcgcc caggggctga
    79141 tgcaggtgat gacgcagatc cactctgaca agtacgagca tttcggcggt cagtacgcgg
    79201 ccttcgaccc caagaccaac ctgcgcgtgg gcgtgaaggt gctgcaggaa tacatcggcc
    79261 gcaccggctc ggtcgaaatg ggcctgaagt ggtatgtggg ggcggccaac ctggccagcg
    79321 acggcggtta cgccgacaag gtgctggccg aacacgcccg cctgtaccag gtcgccaagg
    79381 gcaagccgct gccggcggcg ctgcccggcc tgcgcgcgca gaagccggcc cctgccagcc
    79441 cgcccgaacc gacggcgccg gtcgaggtcg ccaccctgtc ctgaagccgt ggccgtggcg
    79501 gccctaggct aaactgacgc ccgtgcgcaa ctggcgatag gcgcgcggcc cacgggccgg
    79561 cgtcgacgtg ggccaccacg gggaagcgca ccgcccgccg tgcgcggcag gcgcgttccg
    79621 ccgttcgcct gggccgcccc ggtctgacac cgggagcgac gtcaccccaa ggaccgccat
    79681 gttcaaccgt tccagccaca ccatcgccca caccgacccc gagctctggg ccgccatctg
    79741 cgccgagcac cagcgccagg aagaccacat cgagctgatt gccagcgaga actacgcctc
    79801 gccggcggtg ctggccgccg ccggcacgca gctgaccaac aagtacgccg aaggctatcc
    79861 gggccgccgc tactacggtg gctgcgagca tgtcgacgtg gccgagcagc tggccatcga
    79921 ccgcgtcaag cagctgttcg gcgccgaggc cgccaacgtg cagccgcact gcggcgccag
    79981 cgccaacatt gccgccttca tggccttcct caagccgggc gacaccatca tgggcatgag
    80041 cctggccgaa ggcggccacc tgacccacgg catgccgctg aacatgtcgg gcaaatggtt
    80101 caacgtggtc agctacggcc tgaacgcgca ggaagccatc gactacgacg cgatggaaaa
    80161 aaaggcgcac gaaacccgcc ccaggctgat catcgccggc gccagcgcct acagcctgga
    80221 gatcgacttc gcccgctttg cccgcgtggc caaggacgtg ggcgccatct tcatggtcga
    80281 catggcgcac tacgccggcc tgatcgccgg tggctgctac cccaacccgg tgccgcacgc
    80341 cgacgtggtc accagcacca cccacaagag cctgcgcggc ccgcgctcgg gcttcatcct
    80401 gatgaaggcc gaacacgaaa aggccatcaa cagcgcggtg ttccccggcc tgcagggcgg
    80461 cccgctgatg cacatcatcg ccgccaaggc ggtggccttc aaggaagcgc tgcagcccga
    80521 gttcaagcaa tacgcggccc aggtgcgcaa gaacgccgcc gtggtggccc agacgctgac
    80581 cgagcgcggc ctgcgcatcg tcagcggcgg cacgcaaagc cacgtcatgc tggtcgacct
    80641 gcgcgccaag ggcatcaccg gcaaggaagc cgaggcggtg ctgggcagcg cccacatgac
    80701 gatcaacaag aacgccatcc ccaacgatcc ggaaaagccc atggtcacca gcggcgtgcg
    80761 catcggcacc ccggccatga ccacgcgcgg cttcaaggaa gacgaagcgc gcgccaccgc
    80821 gcacctggtg gccgacgtgc tggaccaacc gcgcgacgaa gcccacatcg ctgccgtgcg
    80881 cgccaaggtg gccgagctga cgaagcgctt tccggtgtac ggcaactaaa aaacccaccg
    80941 ccctacgcaa aggacgcaaa ggattcgcag aggacgcaga agaaacccaa atgaatttct
    81001 tgcgtcctct gcccaatctc tgcttctctg cgtacgctag ttcttgatcg gtacgcctgc
    81061 agatgcgctg ccccttctgt gcccacaccg agacccaggt ggtggacagc cgcgtgtcgg
    81121 acgagggtga cttcatccgc cgccggcgcc agtgcgcgca ttgcggcaag cgtttcacca
    81181 cctacgagcg cccggacgtc agctacccca gcatcatcaa gaaggacggc cggcgcgtgc
    81241 cctatgcgcg cgccaagctg cagggctcgc tgcaactggc gctgcgcaag cgcccggtca
    81301 gcaccgaaca gcaggacgcc gccatcgaac ggatcgagga aaaactgctg gccaccggcg
    81361 cgcgcgaagt ctcgtccagc cgcatcggcg agctggtgat gcgcgagctg aaaaagctcg
    81421 acaaggtggc ctacgtgcgc tttgccagcg tctaccgcag cttcgaggac atcgacgagt
    81481 tccgcgcgct ggtcgacgaa gtcaagcgct aaaaaagggc gtgcacccgg cgcacgccct
    81541 tcagggtgcc ggccgccctc aggcgcgtcg acgcaggcgc cgcatccccg tgccgcccgc
    81601 gcgcacgctc agcgcgccag ctgcgcccgc gcccactggg cgatctgggc cgggcnnnnn
    81661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    81721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgca cgctcagcgc gccagctgcg
    81781 cccgcgccca ctgggcgatc tgggccgggc tggtcagggc gcccggctgg cgcgccactt
    81841 cgcgcccgtg cacaaacagc gccagcgtcg ggatgctgcg gatgttgtag cgcgtgccga
    81901 gcgccggcgc ggcctcggtg tcgaccttgg cgacgcgaaa ctgcggctcc agcagcgccg
    81961 cggcctgggc gtaggccggc gccatcatgc ggcaggggcc gcaccagggg gcccagaagt
    82021 cgaccagcag cgggatctgg ctgcgcgcaa ggtgccggtc gaacgcggcg gcgtcgtcca
    82081 gcgccagcgg ctggccggtg aacagcggct gcttgcattg gccgcaggtc ggcgcgctgc
    82141 ccagctggcc ggcgcgcacc cggttggtgg tgtggcagtg ggggcagacc acatgcaggg
    82201 tgtcggtcat gggcggtcct cggcgtccga tggggtgccc ggcgcggcag cggcacgcgc
    82261 cgcggcgatg gcctggcggg cctgcagctc ggcgcgcatg gcggcgccca gcggcgtgcg
    82321 gcacaggccg gcttcgccgt agtgcaggtt ttcatacagc gcggcggcgg cggggctgtc
    82381 ggccagcagc ggctgccagt cgacctcggg gtcgctcagc agcgcggcca ggcgggccgc
    82441 cacgccctgg tactggtgcg cgtccaccgg ctgtcggctg gcgtccagcc gctccagcag
    82501 ccgcgccagc acgatggcgg tgcgtggggg tgagagcagc gatttcatgc ccctggactt
    82561 gggggcgtcg cgcgccattg caagagcggc gcaggcgtcg tccggcgcgc cgattgctat
    82621 gctattggta gctgttggcg cttgatgggt gtctgtcagg ccagttttga tataagcatg
    82681 gcgtgccctc actgcaagga gacgacgcat ggccaccttc accctgtcca gccccgacat
    82741 tcccagcggc ggcagcgtgc cgcggcattt cgagttcgac ggctttggct gctcgggcga
    82801 caaccgctcg ccggtgctgc gctggagcgg cgcgccggcc ggcacccgga gcttcgcggt
    82861 caatgtctac gaccccgacg cgcccaccgg ttcgggcttc tggcactggt acgtgatcga
    82921 tctgccggcc agcgtgagcg agctgcccgc caacgccggt gccctgggcg gcgccctgct
    82981 gccggccggc gcgcgccaga tccgcaacga ctatggcgcc tacgcctggg gcggcgtgtg
    83041 cccgccgccg ggagacccgc cgcaccgcta cgtgttcacc gtgcacgcgc tgtccgtcga
    83101 gcgcatcgac gtgccggacg acgcgcccgc ggcgctgacc ggcttcatgg tcaacgccca
    83161 cacgctggcc aaggcctcgt tcaccgccac ctacggccgc tgagcgccgg tctgggcgag
    83221 acctggcgcc gccgcggcgt cagcccggcg ccggcaggcc gggcacgaag cgcatcggct
    83281 gcgcgctgcg gccctgcggc gcgcccgccg gcggcaccag atcggccagg gtgacgccgt
    83341 cgagcaccgc cagatagccc tgcatggcgc ggcccagcac gttcttgaga tggcagtggc
    83401 tgctcagccg gcaggtgttg aggtcggcgt tgaagcactc gaccagctcg aagtcccctt
    83461 cagtggcgcg caccacggcg cccaggttga tctgttcggc cgggcgcatc aggcgcatgc
    83521 cgccaccgcg cccgcgcgtg gtgtccagca ggcccagcgc ggccagctgc tgcacgatct
    83581 tggtcaggtg actgcgcgag atgccgtgct tttcggccac ctcgctgatg gtcaccggct
    83641 gcgcccgccc gtggcaggcg gcgcagtaca tcagcacgcg cagggcgtag tcggtccact
    83701 gggtcagtcg catggtggtg tcctcggacc aaaagattca cacgcgatga attttagtca
    83761 ataaaatagc catatccgat gaacctttga ggaagccccg atgaacgcca ccacccaagc
    83821 cacccgccct gccctggacg ccgaccaggc gatcggccag atcgccgttg aactgcccgg
    83881 ggcgacggcg gtgtttcgcc gcctgaagct ggacttctgc tgcggcggtc agctcagcct
    83941 gcgccaggcc agcgccgaca aggggctgga tctgcaggcg gtgatgaacg tgttgtcgac
    84001 cctgcagcgc cccagccagc tgcccgacac gggcacgccg ggcgagctga tcgaccacat
    84061 cctgacgcgc tttcacgacg tgcaccgcca gcagctgccc gagctgatcc gcatggcgca
    84121 ccgggtggaa gccgtgcacc gcggcaaccc cgacgtgccc gccggcctgg gcgacgcgct
    84181 ggaggccatg cagcaggaac tgctgacgca catgcacaag gaagaggcga tcctgttccc
    84241 catgctgcgc cagggcggca acagcttcgt cagccagccg atcggcgtca tgcgccacga
    84301 gcacaacgac cacggcgccg cgctggagca cctggccagc ctgacgcacg acatgactcc
    84361 gccgatgggc gcctgcaaca cctggcgcgc gctgtacacc ggcctggcgc aactgcgcga
    84421 cgacctgatc cagcacatcc acctggaaaa caacgtgctg ttcccgccct tcgaagcggc
    84481 caccgccggc ggctgcggct cctcgggctg cggctgcagc tgagcgcggc gccgcggcgc
    84541 gcccggtagg ccgcgcctca ataatgcggc ggcaactcgt cgcgcaggct gcgcggcgcg
    84601 gcgccgctgc cttcggacag ctgctggcgc agctgggtca attcgcgctg cagccgctcg
    84661 atctgctgtt gctggcggta gaccgtgagg ttgagctgct ccagcaggtc ttcggcaaac
    84721 gcggccttga cctccagcgc gtccagccga tcgtcgaccc gcgcggtgtc ggccatggcg
    84781 ctcagcccgc cagcgcctgg gccagtcgcg ccacgccttc gcggatgcgc tcttcgccca
    84841 cggtggcgaa cgacaggcgc agcgtgctga cgtcgggctg ggcgcagtag aacggcgccc
    84901 ccggcacgaa ggccacgccg cgttcgatgg cgcgccgcgc cagctcgttg ccgtcgcgca
    84961 gcgcgccgcc agcgccggtc aggcgcgccc acacgaacag cccgccctgc ggttgcacga
    85021 attcgagcgc cgcgcccagc tcctgccgca gggcgtcgcc catggcacgc gcccgcgcgc
    85081 cgtagacctg gcgcacgcgc gccagcgtgc ccggcatgcg gccggcggcc aggtactgcg
    85141 ccgccgtggc ctgggcaaag gtgctggtgt gggcgtcgga aaactgcttg cacatggtgg
    85201 ccttggccag cagcgccggc ggcgccacca tccagcccac gcgcaggccg ggcgacagca
    85261 ccttgctcag cgagccacag tgcaccagcc agtcgcggct gccgggcacc tgcgacgaca
    85321 gcgccagcag gctgggcggc ggcggcgcgt cgaagtacag gtcgccatac gggtcgtcct
    85381 cgatgatgag cgtctggtac tgcaccgcca gctccagcac gcggcggcgg cgcgccaggc
    85441 tgagcatggc gccgctgggg ttgccgaaag tggggatcag atagacgaac ttcggccggt
    85501 gcgtgcggat cagccgctcc agctcgtcca cctgcacgcc ctcgccgtcg atcggcgcgc
    85561 tgaccagatc ggccccatag agcctgaaac actggatggt ggccagaaag gtcggtccct
    85621 ccacgatggc cttgctgccc ggatcgacca ggcatttgcc cagcagatcc agcgcctgct
    85681 ggctgccggt ggtgacgatc agctgctcgg gtgtcagctc ggacacgccc ttgctgcgca
    85741 tgaaggcggc cagctgttcg cgcagcggcg gatggccctc ggtggcaccg tactgcaggg
    85801 ccgcgccggg ttcggtggcc agggctgcgt tcacggcttc gccgatgccg gccacgtcga
    85861 acatggcgct gtcgggaaag ccgccggcaa agctgatgat gccgggcttg cccagcagct
    85921 tgaacagctc gcggatggcg gaggtttcga cgtgctctag gcgctgggca aacggaaggc
    85981 tcatgggtca acctgtggaa cgggagcctt attgtgacgc ggcgcgcgct ggccataatc
    86041 ggcgccatga ccccggccca gatcgaccgc ttcttcacca ccttgcaggc cgccaacccg
    86101 ctgccggcca gcgagctgga atacaccagc gtgttcgagc tgctgacggc ggtgctgctg
    86161 tcggcgcagg ccaccgacgt gggggtgaac aaggccacgc ggcggctgtt tccggtggcc
    86221 aacacgccgc agcaggtgct ggcgctgggg ctggagcggc tggagagcca catcaggacc
    86281 atcggcctgt accgcagcaa ggcgcgccac ctgatgcaga cctgcgccat tctggtggaa
    86341 cgccacggcg gcgaggtgcc gcgcacgcgc gaggcgctgg agcgcttgcc cggcgtcggc
    86401 cgcaagaccg ccaacgtggt gctgaacgtg gccttcggcg agccgacgat ggcggtcgac
    86461 acgcacatct tccgcgtcgg caaccgcacc ggcctggcgc cgggcaaaac gccgctggcg
    86521 gtggagcagc agctgctgcg ccgcgtgccc gagcgctttc tgccgcacgc gcaccactgg
    86581 ctgatcctgc acggccgcta cgtctgcctg gcgcgcaagc cacgctgctg ggaatgcgcg
    86641 gtggccgact gctgcgactt ccagccgaag acgccggcgc cgtgacggcg ccccggggcg
    86701 ccgcgccgat tcataagcaa aaaacgcctc tggcgcgctt gcatcaagcg ccagcagcta
    86761 tcgttttgat agtttcctgg gccgccgggg cggccgccag cgcggcgatc cgttcggccg
    86821 tccagcccag ctcggcgagg atggcggcgt tgtgctggcc cacggcgggg acttgttcca
    86881 tgcggtattc gaacgcgctg ctgacgcccg gcggcagcag ggccgggatg cggcccgcgg
    86941 gggtgtccac ctcgcgccag cgctcgcgtg ctgcaagctg cgggtgcgcc cacagccccg
    87001 ccatgtcgtt cactcgcgca ttggcaatcc ctgccgcatc caggcgctcg accacctggg
    87061 ctgcggtgag cgtcgcgaac tcctgcagga tcagcgcctg cagctcatcg cggtgctggt
    87121 tgcgcgcggc gttggagcaa aagcgtgcat cgacggccac ctcggggcgc cgcagcacct
    87181 gctcgcaaaa ggcctgccat tcgcgctcgt tctgcaggcc cagcatgacc gtgccgccat
    87241 cacccgcggc aaacggcccg taggggtaga tgctggcgtg cgatgcgccg gtgcgcggcg
    87301 gtggtggggc gccatcgaag gcgtagtaca tgggatagcc catccactcg cccatggcct
    87361 cgagcatgga gacgtcgata tggctgccct cgccggtctt gccgcgcagc agcagggccg
    87421 acaggatgtt ggtgtaggca tacatgccgg cggcgatgtc ggccaccgag atgccggatt
    87481 tggacggcgt ctcgggcgtg ccggtgacgg acagaaaacc cgcctcgctc tggatcaaga
    87541 ggtcataggc cttcttgtcg cggtacggcc cgttggcgcc atagccgctg atgtcgcaga
    87601 cgatgagcct ggggttgtgg cctttgagcg cctcgtacga cagccccatg cgcgcggccg
    87661 cgccgggcgc gaggttctgc accagcacgt cggcggtttt caggagctgc aagagcgcgg
    87721 ccttggcctc ggtttgcttg acgtccagcg ccaggctttc cttgttgcgg ttgagccagg
    87781 tgaagtgcga cgactgaccg cgcacgcgcc ggtcgtagcc gcgcgcgaag tcgccgccgc
    87841 cggggcgctc gaccttgatc acgcgcgcgc ccaggtcggc gagctggcgc gtggcaaagg
    87901 gcgcggcgac cgcgtgttcc agggagatga cggtgatgcc gtcgaggggg cgggtcatg
//
LOCUS       DAOQTZ010000002        61299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_547_length_61299_cov_13.977892, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000002 DAOQTZ010000000
VERSION     DAOQTZ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 61299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61299
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_547_length_61299_cov_13.977892"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..2585)
                     /locus_tag="PKN51_00400"
     CDS             complement(144..2585)
                     /locus_tag="PKN51_00400"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HNT83584.1"
                     /translation="MVSTPPPPSPHRLAQQVRERFLQVLDAAVIELGERIQTHLTGQA
                     QGGIRGATLADMQRAMEAEAAFRRARTAWVTAARRHWRDALTQMTDSRAARRSLPGDL
                     GLVDDDSVERKIAASRLGAAVQDAAGAEFNDLRLRMQALERSTDLPARDVLRPDTFAQ
                     QLLKAWRDAGLDRPMWVLVHPEIQAAMIERLVPLYGQANAFLIERGVLAEIDLKALVR
                     RGDGDAGPVAGARASAVAGARDRAAPGPAENAASRESGARSGGAGLFGDGRSARGDAF
                     TTHGRANAADDTQTPDVRHSALARAAQETRMMTAISPMTRVRQRAHGVLGQLRRLLTD
                     QLANFDLAAPAEPPSPPLARAMAEAAPFAVTELLDRSDADVTIVQPVASVEAVATRLR
                     QRATELKAKAERPSEKATIEIVALMFQAILAEERIAAAIRVWFARLQIPVLRLALAEP
                     DFFASIQHPARQLIDRMGACALGFDAAQVGGSRLEREIKRIVQVIEQYPETGRRVYQL
                     VLEEFKKFLGRSLTEGGSAQQMATLAQQIEQKEALTVQYTIELRRMLEPVPVPEPVRE
                     FLFRVWCEVLAMAAVRRGPQAPDTLRFKQAAADLLWAVSPKADRSERGRVVQQLPTLL
                     HALREGMEGLALSTSDQDAHIKVLNDAVVQAFVSRGEGLSAERLAELSRNLAGLEDVV
                     SDDAQGDVLLDPGVIELMFGAEGDALEVIASGGSQPGEGMLHWAGELELGAWFTLDYL
                     GSVTQVQYVWRSKRGQLHLFGDGSGKSYLVQTWRLASYLQAGLLVPVEDETLTVRATR
                     EALARLNAEPDRLLN"
     gene            complement(2644..3594)
                     /gene="secF"
                     /locus_tag="PKN51_00405"
     CDS             complement(2644..3594)
                     /gene="secF"
                     /locus_tag="PKN51_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNT83585.1"
                     /translation="MEFFRIGRDIPFMRYARLFNAISIVTFLLAVVFLFTRGLHLSVE
                     FTGGTVMEVAYSQPADLAQVRGTVAALGYGDVQVQNFGTARDVIIRLPVQQGKSSAQQ
                     SEQVLAALRSASPDVQLRRTEFVGPQVGDELARDGLYALAFVVVGIMIYLSMRFEWKY
                     AVAGIIANLHDVVIILGFFALFQWEFSLSVLAAILAVLGYSVNESVVIFDRIRENFRR
                     YRKMSTVEIINNSITANISRTIITHGSTQIMVLSMLLFGGETLHYFAVALTIGILFGI
                     YSSVFVAAAIAMWLGIRREDLVKTGRKDGDPGDPNAGAVV"
     gene            complement(3635..5494)
                     /gene="secD"
                     /locus_tag="PKN51_00410"
     CDS             complement(3635..5494)
                     /gene="secD"
                     /locus_tag="PKN51_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNT83586.1"
                     /translation="MNRYPLWKYIVILITLVVAALYTLPNFFGEAPAVQVSAGKATTR
                     IDGGTLSRVEGALQAAGLTPQRLTLEGSSVWARFDTPDTQLKAKDVIERALNPDAADP
                     AHIIALNLVSRSPAWLAAINAQPMYLGLDLRGGVHFMLQVDMRTAITKRLDTLANDIR
                     VALREKDVRHGGISREGNQIEIRARDADTLEAARRVIVDTQPDLQVEPTQGTEPRLVA
                     SFKPQALQRVQADAVKQNITTLHNRVNELGVAEPVIAQQGVDRVVVQLPGVQDTAKAK
                     DILGRTATLEMRLVDESAEGRAAETGGPVPFGSEKFLERDGRPVIVKRQVILTGDSLT
                     DAQPGFDQQTQQPKVDLTVDAKGGRVMRDVSRENLKKRMAIVLFEKGKGEVLTAPVIQ
                     AELGTRFQISGSMSVVEANDLALLLRAGSLAAPMEIIEERTIGPSLGADNISKGFHSV
                     MWGFLAIVVFMIAYYMLFGVFSAVALSVNLLLLVAILSMLQATLTLPGMAAVALTLGM
                     AIDANVLINERVREELRGGASPQAAIHAGYDRAWATILDSNVTSLIAGLALLAFGTGP
                     VRGFAVVHCIGILTSMYSAVFFSRGLVNLWYGRQKKLKSVSIGTVWRPAPEKV"
     gene            complement(5559..5888)
                     /gene="yajC"
                     /locus_tag="PKN51_00415"
     CDS             complement(5559..5888)
                     /gene="yajC"
                     /locus_tag="PKN51_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNT83587.1"
                     /translation="MFISNAYAQAVPAAPGADLASTFTSMLPLVLMFVVLYFIMIRPQ
                     MKKQKEHRAMIDALAKGDEVVTAGGLMGRVTALSEGQIGLQIASGVEVQMQRSAIVQV
                     LPKGTLK"
     gene            complement(6113..9718)
                     /locus_tag="PKN51_00420"
     CDS             complement(6113..9718)
                     /locus_tag="PKN51_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNT83588.1"
                     /translation="MNAPLPEHIRKALETVTLDDKYSLEHGRAFMSGIQALVKLPMLQ
                     RQRDALAGKNTAGFISGYRGSPLGGYDQALWAARKHLQGQHIVFQPGVNEELAATAVW
                     GTQQLGFAPPGSQRFDGVFGIWYGKGPGVDRSVDVFKHANMAGTTPWGGVIAVAGDDH
                     LAKSSTAAHQSDHIFKACGFPVFFPASVQDILDLGVHAFAMSRYAGVWAGMKTIQEIV
                     ESSASALIDPERVQIALPTDFDMPPGGVHIRWPDAALPQEERLFNTKWYAALAYVRAN
                     RLNRNVIEGPNDRLGLIASGKAYNDTRQALLDLGLDDAACRQLGIRLHKVAVVWPLEA
                     QITRQFAAGLREILVIEEKRQVIEYQLKEQLYNWRADVRPNVLGKYREGAAGDYSGGE
                     WSLDNPTANVLLRATADLSPALIARAIAERLKKLGIDADTTARIDRQLAILDAGERNL
                     QVLQASSSGADRQPWFCSGCPHNTSTRVPEGSRAMAGIGCHFMSIWMDRATVGFTQMG
                     GEGVPWVGQQPFSNEQHVFANLGDGTYYHSGSLAVRQSVAAGVNITYKILYNDAVAMT
                     GGQPIGERPEGLSVIQIAQNMRAEGAVKITVVTDEPEKYAQARQHGLPEGIDVQHRDR
                     LDAIQREFREIAGTTVIIYDQTCATEKRRRRKRGTMVDPAVRVLINEAVCEGCGDCSV
                     QSNCLSVEPLDTEFGRKRTINQSTCNKDISCVKGFCPSFVTVEGGQLRKKAKARASVS
                     PFELGALPEPPLPQPEAGGAWGIVVAGVGGTGVITIGQLLGMAAHLEGKGIVTQDAAG
                     LAQKGGATWSHVLIGATQDDIRTTRVSAAAADLIIGCDPIVTANKETVARMREGRTHV
                     ALNAHATPTAAFVKDGGWQNPQARCAADIARAVGEQGLASFDADRLAGRLLGDTIYVN
                     PMILGYAWQRGWVPLRRESLLRAIELNNVQVDNNKAAFEWGRQAAHDGARVEQALAGA
                     GGEQVVAFKPRETLESIVARRVEFLTGYQNAAYAERYRAFVARVQQQEAALGRTQLTE
                     AVAQGLFKLMAYKDEYEVARLHSDPAFLARIDAMFEGDYKLHYHLAPPMTAQRNERGE
                     LVKKKYGPGMGRVFRLLAGLKGLRGGALDLFGKTDERQTERALIEQYRASIDELLATL
                     NADNHAIAVEIARIPQAIKGFGHVKERHLKAARARWDELRAQYRQPPVAPARQIA"
     gene            9874..10368
                     /locus_tag="PKN51_00425"
     CDS             9874..10368
                     /locus_tag="PKN51_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNT83589.1"
                     /translation="METLDAHDLAILEQLQADGRLTNAELAQRVGLSAAPCWRRVRAL
                     EQAGFIRGYRAEIDRHKLGLGVLAFVRLDAAQVSGAALRALEAAIRTRPEIVSCHYIS
                     GTGTFELQVVSTDLEGFSRFAREVLLNLPNVKDMHTSFSLGEVKAAGALPLEHLRAKP
                     RRGG"
     gene            complement(10379..10957)
                     /locus_tag="PKN51_00430"
     CDS             complement(10379..10957)
                     /locus_tag="PKN51_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83590.1"
                     /translation="MSRARAPEAGRRAAWAGAARPASMFKAKMAAPPARSAPEATLFI
                     AFRRLAGGLALAALGGCASWQDAPRLAYQCPHELRFEARLYQDMALLEGLRGHAVLER
                     VPGAEGDALHYADPTVRARFGLGLDARLVRLDYTGIPEPVYCQHLPGADAPAAAVRAS
                     PRLGPRPPPPRPDPDAPVQTNIRQGDGRNGPG"
     gene            complement(10954..12111)
                     /locus_tag="PKN51_00435"
     CDS             complement(10954..12111)
                     /locus_tag="PKN51_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HNT83591.1"
                     /translation="MQRVSRILAAAAMAALLAACALRPPPAPAPLPPAEPGLPPAGPV
                     ADTAPLGPPIERARSRWVPVRWAELPGFEQDDTAAAWNAWLKSCERPGPTFARLCSEV
                     RRLALGSTQQQRDWMRARLQPYRVEPLAGAPAGDGLLTSYFEPVYEASRVARPGFGAP
                     VYRAPPGASQRKPWYTRQELDTLPEARAQLAGHELVWLADPLDALILQIQGSGRVRVQ
                     EADGSQRLLRLAFAGTNEHPYRSPGKWLIEQGLLRGDATWPGIRAALAAQPQHLQGFL
                     WSNPRVVFFREEPLSALEAQFGPRGAQGVALTPGRSIAVDPQSIPYGTPVWLSSQGPV
                     AQLQRLVLAQDTGSAIVGAVRADYFAGWGAEAGELAGRMKQPLRLWVLWPR"
     gene            12110..12235
                     /locus_tag="PKN51_00440"
     CDS             12110..12235
                     /locus_tag="PKN51_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83592.1"
                     /translation="MTLILLEALGALLLFVLLVWWTMFAGRKKGELPEPADDDAE"
     gene            complement(12273..12893)
                     /locus_tag="PKN51_00445"
     CDS             complement(12273..12893)
                     /locus_tag="PKN51_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT83593.1"
                     /translation="MSAADAMVYIVDDDAEVRAALAWLLRSRRLCSEGFDSAQAFEQA
                     LGEAPPQTPACLLLDVRMPGTSGLALFDRLLERGIATAWPVIFLTGHASVATAVEAVK
                     RGAFDFCEKPFSDNALVDRVERALAVSRERVAQLGAQRALRARLDELTEREREVLQRV
                     ASGRPNKLIADELGISVRTVEVHRARVFEKMDVKSAVELAQLLQQI"
     gene            13093..14286
                     /locus_tag="PKN51_00450"
     CDS             13093..14286
                     /locus_tag="PKN51_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT83594.1"
                     /translation="MSTPFLRRLVMPATLCLAAALSACGGGGDDDATPPPPAPPGQAS
                     GSLLSSAPVNTWSAEQIAQALSDPDSKVQSGVTPRYAVRSHRVTYVSSDKHGQPVTAS
                     GLISVPLKPPGARSPVLSYQHATTFHDDQAPSNKVEAVEPPIVLASLGYIVLSADYVG
                     FGAAKGLEHPYLTAAPSAAVVLDLLHAGQTWRQQQAVADNGQLFLAGYSEGGYVTLAA
                     QRAIETTGDALRQQLQASVPGAGPYDVQATLDAQVEAVRAYNSTLGKLFEPHRLSQAP
                     EWVRNELRRLLLRQMVPDDADVRYQTLFLDRYMADDQAAIAAEHSVHLGWAPAVPVYL
                     FHGRDDRTVPYAAATSALNTLRAAGARDISLTDCTTPDYGHLKCVPEYFRFAVERMGR
                     LARDL"
     gene            complement(14322..14804)
                     /locus_tag="PKN51_00455"
     CDS             complement(14322..14804)
                     /locus_tag="PKN51_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNT83595.1"
                     /translation="MSSIDRIDRKILDQLQRDGRISMTELAQQVGLSTSPCAERVRRL
                     ERSGVIQGYHARVNPEALGRGLLVFVELTLSSKSAEVFRQVRDELLLEPRVLECHLVS
                     GTFDYLVKARLSAMREYRDLLGQILRKMPVPAQSNSYVVMEEIKEGLALPVDPVPVGA
                     "
     gene            14934..16232
                     /locus_tag="PKN51_00460"
     CDS             14934..16232
                     /locus_tag="PKN51_00460"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNT83596.1"
                     /translation="MKVIVLGGGVIGVSTAYYLARAGAEVTLLERQDDVALETSFANA
                     GQVSPGYSTPWAAPGIPLKAVKWLFQKHAPLSIRPDGTLFQLRWMAHMLRHCTSARYA
                     VNKERMTRLAEYSRDCLRQLRADTGIEYEQRSLGTLQVFRTAAQLQAARRDTAVLQEC
                     GVPYRLMTDVAELAEYEPALARARGLVGGLQLPGDETGDCRRFTTALAEQARALGVRF
                     RLGVQIAELLPRGGQIGGVRLASGEVLSADRYVLALGSCSRALLAPLGLDLPVYPVKG
                     YSLTVPLLDPANAPRSTVMDETYKVAITRFDQRIRVGGMAELGGFDLRLNPRRRETLE
                     LVLGNLFTGGDIPRAEFWTGLRPMTPDSTPVVGATRYANLLLNTGHGTLGWTMAAGSG
                     KLVADLTLGRQSDISTAGLGLERYGRSQAGPRSLPRHVPA"
     gene            complement(16555..17910)
                     /locus_tag="PKN51_00465"
     CDS             complement(16555..17910)
                     /locus_tag="PKN51_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNT83597.1"
                     /translation="MSVTATVPAPAATRRDAAWLEAHWMPFTGNRQFKRDPRLIVAAQ
                     GAYFTDADGRQVFDGLSGLWCSGLGHGRREIAEAVGRQAAQLDYAPAFQFGHPLSFEL
                     ANAIKQRTPQGLDHVFFTASGSEAADTALKMARAYWRARGQGGKTRLIGREKGYHGVN
                     FGGISVGGMVGNRKLFGQGIEADHLPHTQPPAGTFAKGQPAEGGVALADRLLELIALH
                     DASNIAAVIVEPFSGSAGVVIPPVGYLQRLREICTQHDILLIFDEVITGFGRCGAMTG
                     SDAFGVVPDLMTFAKQVTNGVQPLGGVVASRAIYDTFMAAGGPDYMLEFAHGYTYSAH
                     PVACAAGLAALELLEREDGPGRVKALSAHFEAAVHSLRGARHVLDIRNYGLAAGFTIA
                     PVAGEPARRPYDIAMACWKKGFYVRYGGDTIQLAPPFISEKAEIERLVNALGDALAEV
                     A"
     gene            18021..18983
                     /locus_tag="PKN51_00470"
     CDS             18021..18983
                     /locus_tag="PKN51_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT83598.1"
                     /translation="MQVNPTPARSRAVLGQVSDMDLRLLQVFKAVVECGGLSAAELEL
                     NIGTSTVSRHVKDLETRLGLTLCRRGRGGFAVTPEGQRVYDETRRLLAAVDGFRASVD
                     DIHQRMGGTLAVALFDKTVSNPQARIHAAIADFTQQAPDVALHLHVGSIQTIERGVMD
                     GSFAVGVIPAHRASASLRYQRLFGETMHLYCARPHALFDSEHSALDWPALRARQDFAG
                     LGYHSPNMELAHGQRLRRGATAYDQEAIATLILSGRFIGFLPDHYAAAFVQRGQLRAV
                     APERLHYDCDFVSVLRRSPQPARAALAFHRALLAAHADAMTHDA"
     gene            complement(19033..21087)
                     /locus_tag="PKN51_00475"
     CDS             complement(19033..21087)
                     /locus_tag="PKN51_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT83599.1"
                     /translation="MSATAPVRAAASPDPGSAAALRRLRAGAGRRWLLWGVLSLTVVG
                     LLAIAVWLARGHEIEQVQRALERDSLEAVQVLRERLGRNLQDLHGLGAAPADPEAWSA
                     GAARLLETHREWLRLEWRGAALEPRAARDSPFHTPLPAEVREAMAPELQRLCQQASVQ
                     GEAIYAPPSFVLRAGGLGREVMTLCLPLQDGARTQGFVLASYTLDELLRLLPTSLTRG
                     QAVAFTELDGTRLALHGAARRGARLFVAQQTLDLPGAPMLLRLEGGRPLPDVFPNVLT
                     ALVTLLSMALLTALGLLARDFRRRQQAEAELAEALAFRKAMEDSLITGLRARDLQGRI
                     TYVNPAFCAMVGYTPQELIGQSTPAPYWPPERAHEYGARQALRLSGQAAAPREGVETE
                     FMRKDGTHFPVLIFETPLITAQGQHSGWMGAVVDMTAQRRAEESSRASLERLQASARL
                     ATVGEMASLMSHELNQPLAAIASYASGSLNLLPAGAGGDAATARELRTALERIAEQAG
                     RAGRVIHSVRELVRQRSTAPQAVAPRALVDAVLPLVQLQARKLGASVRVQVAPELPAV
                     WCEPTMIEQVLLNLARNGLQAMAEVPGERLLTLQARLPTAPAGPALLEFGVADTGTGM
                     DAPVVERLFTPFFTTKPEGMGLGLSLCRTVIEQHGGTLVHAPNLPRGTLFRFTLAVAD
                     AP"
     gene            complement(21084..21914)
                     /locus_tag="PKN51_00480"
     CDS             complement(21084..21914)
                     /locus_tag="PKN51_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNT83600.1"
                     /translation="MKVAAIQMVSGSRVEDNLHRAQALLAQAAQAGAELAVLPEYFCL
                     MGQRDTDKLALAEPLGDGPIQRWLADSARALGLWLVGGTLPLAPDGAAPPLTHCRNAT
                     LVFSPAGERVAVYDKIHLFQFDDGTRRYDEAAVLRPGRQPVRFDLPSRDGHRWRVGLS
                     ICYDLRFPELYRQHAADGAELLLVPSAFTHVTGQAHWEVLLRARAIENLCGVVAAAQG
                     GTHDNGRRTWGHSMVVDPWGQVLAVQDEGAAVVLAELEPARLAACRARLPALRHRVLG
                     "
     gene            complement(21911..25981)
                     /locus_tag="PKN51_00485"
     CDS             complement(21911..25981)
                     /locus_tag="PKN51_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HNT83601.1"
                     /translation="MRWVVRGVGVLLALVLLAAAILHWVIVPRVDEWRPRLERLASQA
                     IAAPVQIGAIEAQSNGLVPSVALRDVRVLDPDGRPALQVPRVLAAFSVLSLGTLGLEQ
                     LVIDAPELELRRTRDGRLLAGGIDLSGDATGDTRAADWFFSQDEIIVRDGRVRWVDEQ
                     RAAPPVQLSAVLFVSRNGYGRHQLRLDATPEPAWGERFTLIGQFRQRVFSRHPGYWRE
                     WDGQAFASAPRIDVSRLRQYVDLTADWGVDVQAGHGALRLWLEQKQGRPVAATADLAL
                     GAVSVTLGPRLAPLAFSELTGRLAWRDPGDGMTLGTRDLRFVDADGLTWPGGNLRLAL
                     HDAAGRHPGGGEFSGDRLDLAALAKIAQRLPLPPAVHERLQAHPVQGLVERIEARWQG
                     PLEAPTDWRVQTRVSGLALAAPAQATPATGEPVAGIPGIEGAALELEATPAGGRASLA
                     IADGALTFPGVFEEPRIPLARLDLRGRWRVQGEQLAVEVEQLDLRNADATGSFKARWQ
                     TLAGAQGAARFPGLLDLSGSFSRANGARVHRYLPLVIPAEARHYVRDAIVRGDARDVA
                     VRVRGDLRQLPFDQHPEAGEFRFAGPVQGVTMAYVPRALQPAGQPPWPALEGLAGELI
                     FERNSMRVRQASARVQGHPGWRFTRVQADIDDLQHARVRVEAEGRGVLASALDIVRGS
                     PVAGFIEHALDRASASGDASLQLGLDLPIDAIDRSRVNGRVALAGNELRIAPEAPLLT
                     QAQGVVTFSETGFAVADARVQLLGGQARLSGGSTAAAQGGPTVLLRASGSASAEGLRE
                     MADWAPLPALARQATGSASYEAQIGFRGGEPEVRVTSDLRGLAFDLPAPLDKPADAAW
                     PLRYDSGPVAGSAGRQRLRVEVAQRLAIEYEHAADSDRVLRGLIGLGPRATALALPAS
                     GVQAGIELPHLDVERWRDALQRVLGGGATLSAEGGRLPSRWTLRTAELVVDGRSLNDV
                     VASGTQQGGQWRGEVQARELAGRIDFREGADGRAGKLSARLARLSIPAGGSDDRTLLA
                     QPPAQIPALDVVVEQFELRGKALGRLEIQAVNHDVAGPAAAGETLQNWELSRLSLQAP
                     EAHLTAHGRWAALARGPGLPLDPRGPRAPEDRRRTQLEFKLDIRDAGKLLARLDMPDV
                     LARGRGELRGGLGWRGTPFSPHYPSMAGQLHLDVDAGQFLKADPGVAKLLGVLSLQAL
                     PRRLTLDFRDLFSTGFAFDFIRGDVEVQAGIARTNNLQMKGPSAAVLMDGEADLDHET
                     QRLRVLVVPEIDAGTAALVATAINPAIGIGAFVAQLVLKRPLIKAATREFQISGRWDD
                     PQVTPLGAPAADAQTPAPATPPTPRSATDTPEEERS"
     gene            26101..28926
                     /gene="glnE"
                     /locus_tag="PKN51_00490"
     CDS             26101..28926
                     /gene="glnE"
                     /locus_tag="PKN51_00490"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNT83602.1"
                     /translation="MTPADVQGSHGDGESLLSDFRGLPGSAAHSRLVQRLRRRYADEL
                     PLLPAGTPTRASMQDCLTALAARGHALGPRLRILRQLVMERLVVLDCEQGAPLHDITG
                     AVTELAELALDAAFTDAFAQLDERHGEPLTEAGQRPRFWVIGMGKLGARELNVSSDID
                     LIYLYEEDGQTRGRADGRGQISVHEYFGRAARLAQTLIGDTTEHGFVFRVDLALRPNG
                     ESGPIVCSLDALEEYMLAQGREWERLAWLKSRALAPRADLDGDAARALRAVVLPFVFR
                     KYLDYNVFESLRTLHRQIREHASKRSAGRPERANDVKLSRGGIREIEFTVQLLQVVRG
                     GQFPELRTRPTLAALERVAHAGLMPPDTADALARAYVFLRRVEHRIQYLDDQQTHVLP
                     TQDEDLDWIARTLGYPSACPFLADLCAHRERVAHEFDGLLGGDAPCKSCGKKSADAPA
                     PAPHDLPAVLDAFPERVRERLAPWQDHPRVRALREEALGRLLRLLQRTAAWLDEGRVS
                     DDAVLRLADWVEPLLRRESYLALLLERPAVHERLLRVLGTARWPARYVMQHPGVIDEL
                     AGPGLLSGRFDALAFEQELERRRQALASTGEDDDESLLNLLRRAHHAETFRTLVRDVE
                     RRITVEQVADDLSALADAILRLTTCWCWPRLRQRHREQPHFAIIAYGKLGGLELGYGS
                     DLDLVFVFDDEHEDAPDIYAALARKLINWLTAQTREGDLYEIDTALRPNGNSGLLVTS
                     FDAYADYQQNRGSNTAWTWEHQAMTRARLLAPRLAADAAAPGGAAAEPAFDLSARFQA
                     VRHAVLTAPRDHAALRAEIIAMRERLQAAHPVRAGLFDLKHSPGGMIEAEFATQYLVL
                     AHTREHPELEPNLGNIALLIRAEQVGLLPAGVGQGAADAYRHLRHLQHLARLDEQSTQ
                     IDPATVTEQRAAIQRLWQAVFTPASRA"
     gene            29127..29762
                     /locus_tag="PKN51_00495"
     CDS             29127..29762
                     /locus_tag="PKN51_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83603.1"
                     /translation="MNRWQRSAWPLACLLLIVASALAGLAVASGRLPPELLSWRRGDW
                     PHAPWTLWSGALLHRLAPHALANMLALAALAVLGAALGARPRDALALLVAWPLGTLGL
                     RCWPQVAAYWGLSGPIHAAAAVLALRALTAADTRWLGLLLGGGLSVKLALEHGWESPI
                     AFDAGWGFNVVFAAHLSGAIAGALLAVALNVAGWLRLASASATDQRSSHGK"
     gene            complement(29832..31220)
                     /locus_tag="PKN51_00500"
     CDS             complement(29832..31220)
                     /locus_tag="PKN51_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNT83604.1"
                     /translation="MSHPTPYAARRARVAAQLGPDAIALIPTAPARPRNRDSDHPYRH
                     DSYFYYLTGFTEPDAWLVLTSDGRSTLFCPPKDLEREIWDGIRLGPEAAPAALGVDEA
                     YSVHELNARLPGLLENRRSLWFPFAIHEGLSARVDGWLQQVRARVRYGALCPGEQHDL
                     CRVLDELRLIKDAGELATMRRAGQISAAAHVRAMQRSAAMLRAGQDVREYHLEAELLH
                     EFRQHGAQSPAYPSIVAAGANACVLHYRAGDAAIRAGELVLIDAGCELDGYASDITRT
                     FPADGRFAGPQRALYELVLASQQAAIAATRPGARFTDPHEAAVRVLTQGMLDLGLIDK
                     GQHGTLEDAIAHRAYFAHYMHRTGHWLGMDVHDCGSYVEPGEVGQPSPRRDPLSGELI
                     QDRPSRILRPGMVLTIEPGLYVRPSEKVPSAFWNIGIRIEDNAVVTADGCELLSRGVP
                     VEPDAIEALMRA"
     gene            complement(31229..32005)
                     /locus_tag="PKN51_00505"
     CDS             complement(31229..32005)
                     /locus_tag="PKN51_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT83605.1"
                     /translation="MPHPAPRALILAAGRGERMRPLTDRCPKPLLPLRGKPLMQWALE
                     ALLRAGVRELVVNTAWLGQQIEDHFAAQRLSDVRQQLSISEHIGLHFSSEGRDFGGAL
                     ETAGGIVRALPLLAPGARDVFWVAAGDVFAPDFDFAADAVRRFVASDRLAHLWLVPNP
                     AHHPAGDFAIGADGLAQNDAPAGGRRHTFSTIALYRRALFEPPWCDIPAGNPQGVAAP
                     LAPLLRRAIAAGRVGAELYQGPWTDVGTPERLAALNAGVS"
     gene            complement(32158..33924)
                     /locus_tag="PKN51_00510"
     CDS             complement(32158..33924)
                     /locus_tag="PKN51_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNT83606.1"
                     /translation="MNTVVTDVLVIGTGPAGAATALCLARAGVKVLAVSKYHATSPDP
                     RAHITNQRTMEFLRDFGLEEAAKKIAMPWEDMGEHVFGRSLVGEEWGRIPAWASSAIA
                     QGEHMAASPSRYCDLPQLYLEPLLVNDAGLRGADIRFKTEYLSHTQDAEGVTATLLDR
                     VSGSEYQVRAKYLVGADGGRSKVAADIGLEFDGAMGLGETGNINIEIQADLSQFCAHR
                     RSDMYWMLQTGENLMGLPQGPGIAVLRMVRPMDRWVCVVGYALAKGLPSLKHEDLLPF
                     VHRTIGSDQVPVKILSVSTWTTNRQYALTNTQGRVFCIGDAVHRHTPFGGLGSNTCIQ
                     DAYNLSWKLAMVIQGQAGASLLNTYQDERAPIAKQITEHAFNGGASMGPLLMALGVPP
                     GSGPEAMEQAVARLKEPSAEGAERRAKLRQAMDGTLNGFGRAHGIELNQRYRSSAIVS
                     DGTPDPGFARDPVQYHQASSRPGAHLPHAWVTRGQRKISTFDLCGQGRFTLLTGLSGM
                     AWKATAEQAAAALGIELVVHVIGFGQEVVDSHGDYLRISEVEESGALLVRPDLMVAWR
                     AANADSTQLAQLPSVLRQILGR"
     gene            complement(33944..34804)
                     /gene="bphD"
                     /locus_tag="PKN51_00515"
     CDS             complement(33944..34804)
                     /gene="bphD"
                     /locus_tag="PKN51_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
                     hydrolase"
                     /protein_id="HNT83607.1"
                     /translation="MLIHTDEDTSRFITIDEPGLENFQLHYNDAGSGPAVIMLHGGGP
                     GASGWSNFYKNIDHFVDHGFRVLLLDCPGFHKSDELVSDQVRPLLNARAVKGLMDGLG
                     IERAHLLGNSLGGATALHFALEFPERLDRLVLMGPGGLGHSIMQANPQEGIRHMIKLY
                     KQPSYAHFESMLEVFVYNPAAITEELRQGRWANIQARPNHLSNFLRCVALSPIATWDM
                     MARAHQIQHPTLITWGRDDRFVPIDHGLRLLHTLGKAELHVFSQCGHWAQWEHAPAFN
                     ELVINFLKRA"
     gene            complement(34938..35837)
                     /locus_tag="PKN51_00520"
     CDS             complement(34938..35837)
                     /locus_tag="PKN51_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT83608.1"
                     /translation="MASISNLGYLVLGVKDLEAWKQFAVDTIGLQPGRSEPGKLLALR
                     MDGYEQRIVLERHDDDDMLAAGWELDTQEDLNAFAAQLRQQGVAVQQGDAALAKERRV
                     EDLYFCTDPNGIRHEFYVGAQRASMVNPFHSSKLVGGFSTGRLGMGHFVAAANDAQET
                     IAFCKQVLGIKISDFIRGELAPGVLLDVAFFHARTGRHHSMATVKIPFPYPKRIHHIM
                     VEVDNPDDVGLAYDRCVQAGYTINMGLGRHPNDRMFSFYVHTPSGFLLEFGAGGIVVD
                     DADWEIKSYTRLSEWGHHHPEPH"
     gene            complement(35881..36147)
                     /locus_tag="PKN51_00525"
     CDS             complement(35881..36147)
                     /locus_tag="PKN51_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83609.1"
                     /translation="MSTTTCGICRTGARGVLSGMAGRGCCAMIHKNLHEIMKSINFGT
                     WVASDMRGITRGERGKPTKAQRQTEGFPLDLGQSFLAGTVLAEL"
     gene            36146..37939
                     /locus_tag="PKN51_00530"
     CDS             36146..37939
                     /locus_tag="PKN51_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT83610.1"
                     /translation="MKLPQIRELATQLRFAPEEGRIWMDERRMVLVHSDTFADLREEL
                     LASLGYEMTRGLLTRIYFAAGQRDAASVLKRLGPGASLKEVLNTGGRMHALQGYLLPE
                     HVGRGLAAGDLRSDDYYGEALWKDSLEDEAHVARHGVGQHAVCWNAVGYCSGYLSYCA
                     QRTIVVREVECRASGGNHCKIVAQPAAQWDNVEEDLRFLLPQPVGRRARIVAPVADGG
                     PTTALAQSAVTDSRDDDRAAPRSLAETAFVGASTAFSVLRHKIDRVAPTQATVLLLGE
                     SGVGKSLIARQVHRRSQRAQAQLIEVNCAAIPEALVESELFGVERGAFSGASQARAGR
                     FEAAHGGTLFLDEVATLSPTTQGKLLRVLQSGALERLGSNQTRVTDVRVIAATNEDLK
                     QAVRQGRFREDLYFRLNVFPVVVPPLRERRDDIPLLVQALLQRFAPLHGRGHVGITSR
                     ALQALIHHDWPGNIRELENVIERAVIMLHEGEMLDLQHLSYADDTLNTPSYFGLDDTG
                     QLMINTDYDFGASQSPASTSAASPAALDMNRLADDLLRQGPGQLAAMERALVRSAVRQ
                     AGGNITRAAALLGLSRAQTEYRYRKQQSAPG"
     gene            complement(38006..38317)
                     /locus_tag="PKN51_00535"
     CDS             complement(38006..38317)
                     /locus_tag="PKN51_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNT83611.1"
                     /translation="MSQWIDVAAEDDIFEGAAIAVTPIGHDIALYKTEAGEVFATHNI
                     CSHGHARLCEGFLEGHEIECPFHQGRFDVRTGQATLAPCVDPIRSYPVKIEAGRVWVQ
                     L"
     gene            complement(38333..38809)
                     /locus_tag="PKN51_00540"
     CDS             complement(38333..38809)
                     /locus_tag="PKN51_00540"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HNT83612.1"
                     /translation="MRLDFDTWQALVQLQADYAAVVDGGDWDRWPRFFTEDGVYKLQP
                     RENHERGFPLCTLAFDCQAAMRDRVYGIKETLFHDPYYQRHVVGAPLVHAADSDGIRC
                     ESHYAVFRTKLDGLSSVFNVGRYLDRVVRTPLGLKLAERLVVYDSEMIPNSIIYPI"
     gene            complement(38806..40101)
                     /locus_tag="PKN51_00545"
     CDS             complement(38806..40101)
                     /locus_tag="PKN51_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNT83613.1"
                     /translation="MIPSTSLDWPAGDTSRVPFAIYTNEQIHRKELQRFFYRGHWCYV
                     GLEAEVPRPGDFKRTVVGERSVILSRAADGQLHCFENVCAHRGMQFCRKRHGSGMKEF
                     VCPYHQWSYTLSGELQGVPLRRGVRQDGRVLGGMPADFDTKAHGLTRLKVATRGGVVF
                     ASFDHDVEPLEDYLGPTILPYFDRLFNGRQLKILGYNRQRIPGNWKLMQENIKDPYHP
                     GLLHTWFVTFGLWRADNKAALRMDAHHRHAAMISTRGNAGKQSDVSQVSSFKAAMELE
                     DKRFLDIVHEDWWGEPTVVMTTVFPSVIFQQQVNSVSTRHIQPDGHGHFDFVWTHFGF
                     ADDTAEMTARRLRQANLFGPAGFVSADDGEAIELSQCGFTQKPGHHALVEMGGRGVEE
                     TDYMATETLIRGMYDYWRKVMERPDQASDAVAPQEGAQA"
     gene            40219..41136
                     /locus_tag="PKN51_00550"
     CDS             40219..41136
                     /locus_tag="PKN51_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT83614.1"
                     /translation="MDLSSIDLNLLRILHRLLQERHVSRAAQDLGLSQPAVSNALRRL
                     RVQLGDDLLVRSAAGMQPTPLAQRLAGPIAQALELLDDALRAQAPFDPADSVRQFTLA
                     MSDVGELYFLPVLMRALAQLAPGVTLRTTALDSPGLGPSLSTGEIDLALGSLPRLQGG
                     FYQQSLFRQGYLCLMRNGHPMAEGPLTRAAFLACEHVRVEAPGTGHGRVDSEFERRGI
                     RRRIRLSVPNYVALGHVLASTDLIATVPERFAERVCAALALRTHPLPVRIASGVIRQF
                     WHQRSHRDSGHEWLRRQVSSLFADSATRA"
     gene            41330..42892
                     /locus_tag="PKN51_00555"
     CDS             41330..42892
                     /locus_tag="PKN51_00555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNT83615.1"
                     /translation="MTAPCLLAIDCGTQSVRALLFDLQGQLLARAQVAIDSYRAPEPG
                     WMEGAPEAFWQHLCAACHRLWATGTVPREAIAGVVLTAQRAVPVTLDADGQPTRPSMI
                     WLDQRRAARAPRLPWWWEAALTAVGMRAAVRHFQHEAEACWIAQHQPELWARTRHFLL
                     LSGYLNWRLCGRLADSVAAQVGYVPFDYRRGRWAAPWDWKWHALPVRRAMLPELLPPG
                     ARLGTVSAEAAQASGIPAGLPLIAGAADKACEVLGAGCLSPEVACLSYGTAATVNITT
                     ARYLEATRFIPPYPAAVPGHFNPEVQITRGFWMVRWFREQFGQHEERLARERGVAPET
                     LFDELVGTVPPGAQGLTLQPFWNPGIKQPGPEAKGAIIGFGDVHTRAHLYRAILEGLA
                     YALREAAERVERRSGIAIERVRVAGGGSQSDAALQITANVFNRPAERPHLYETSGLGA
                     AIIASVGLGLQPDFASAVRAMTRVGQVFAPQPEHAHTYERLYRRVYLRMYRQLAPLYR
                     DIRDITGYPSRD"
     gene            42905..44254
                     /locus_tag="PKN51_00560"
     CDS             42905..44254
                     /locus_tag="PKN51_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83616.1"
                     /translation="MKRLLITVALSFGAHLSAQAWSNHALASYRAFEPMPEVAQAPAV
                     LAEPLEQFLAAQAQPIADLLQAQDAWAQAHLPHYPPLPAALRFDPAVASQGPQALRRA
                     FLTALRVSPDSRLALYVQPDPWGPEPQAERLPHDAVSALPLRDKDGEKHRFVRLHAGQ
                     AVAPIAVLASASDEPDYGMDLNLWQDSPSSWGPGYGFGHIPFGNPSLDFATQAPFHMG
                     YYHESGLIYAAAGFLKRTYPLLRAHQYQGLAQLAFRRGHPYWGWRFAGLALHYLQDLT
                     QPYHASLAPGYSSARLIGIQLLALLGRPGAKNDMVVRLSNRHLVLERYESQMIQASAQ
                     ARKAGPLEQALRDTAADASYGPWSDTTLRDEVARQAHAAGETVTAQLLATVPAGYVDD
                     PTFDFGVHAGGIDLMAEVAQRGAPAKQALEQTLATLMRHFGVHSRRLVRAILQGAAP"
     gene            44280..44771
                     /locus_tag="PKN51_00565"
     CDS             44280..44771
                     /locus_tag="PKN51_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT83617.1"
                     /translation="MFDVTPLSATDRSEQYAELARQVDGLLHGETQLIANAANFAALV
                     FHALPELNWCGFYFAEGQELVVGPFQGKPACVRIAFGRGVCGTAAATRQTQVVPDVHA
                     FAGHIACDSASRSEIVVPLLRRDGSLLGVWDVDSPRPARFDEVDRVGMQALCARFLRA
                     CAL"
     gene            complement(44798..45247)
                     /locus_tag="PKN51_00570"
     CDS             complement(44798..45247)
                     /locus_tag="PKN51_00570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNT83618.1"
                     /translation="MDPCTSPDIPAGFHPLQAGGGFMAFNGPLYLRHQGDDVRFGLRV
                     EPRHCNPMGNLHGGMMASFCDMLLPLSVHRKGAGLGQRFLPTISLQIDYLAPAPLGCW
                     IEGTAELLRATRTLVFAQGLVTADGEPCARASGVFKIGPEMPTELAG"
     gene            45413..47449
                     /gene="tkt"
                     /locus_tag="PKN51_00575"
     CDS             45413..47449
                     /gene="tkt"
                     /locus_tag="PKN51_00575"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNT83619.1"
                     /translation="MANPSRMANAIRALAMDAVQQAKSGHPGAPMGMADMAVALWTRH
                     LRHNPANPQWADRDRFVLSNGHASMLLYALLHLSGYELPLQELKDFRQLHSKTAGHPE
                     VGLTPGVETTTGPLGQGVTNAVGMALAEKLLAAEFNRPGHAIVDHHTYVFLGDGCLME
                     GVSHEACALAGAWKLNKLIALYDDNGISIDGQVAPWFVDDVPARFAAYRWNVIGPIDG
                     HDVDAVDRAIAQARRSSDRPTLIVCKTHIGKGSPNRQDSAKAHGEPLGTEEIALTRAA
                     LGWDAAPFEIPADVGAAWDARAAGQAREAEWTQRLAAYQAAHPELAAELLRRLRGELP
                     PNFHQVAVDAAVAAHQKAETVASRKASQIALEAFTAALPELLGGSADLTGSNLTNTSH
                     TPALRVDAAGDVVHDAQGRIGRHINYGVREFGMAAIMNGVALHGGFIPYGGTFLTFSD
                     YSRNAIRMAALMKQRVIHVFTHDSIGLGEDGPTHQAVEHAASLRLIPGLDVWRPADTA
                     ETVVAWAVALQQRTLPTALLLSRQNLPYCPRRDLGDISRGAYVLSEPSDLGLKRKAQA
                     VLIATGSEVQLALKAQELLAREHRIAVRVVSMPSTTTFDRQELKYQKAVLPAGLPRIA
                     VEMGATDGWWKYGCAAVVGLDRFGESAPAPVLFEHFGFTPENVADTVRAALRRR"
     gene            47639..48640
                     /gene="gap"
                     /locus_tag="PKN51_00580"
     CDS             47639..48640
                     /gene="gap"
                     /locus_tag="PKN51_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNT83620.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAVANYKDIEVVGINDLLEPDYLAY
                     MLKYDSVHGVFAGDIAVEGNTLVVNGKKIRLTAERDPANLKWGEVGADVVIEATGLFL
                     TKEAAQKHLDAGARKVMMSAPSKDDTPMFVFGVNHDTYAGQAIISNASCTTNCLAPVA
                     KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VLPALKGKLTGMSFRVPTSDVSVVDLTVELDKPASYAEICAEMKAQSEGKLKGILGYT
                     EDKVVATDFRHDARSSIFDADAGIALDATFVKLVSWYDNEWGYSNRCLDMARVMSQK"
     gene            48856..50706
                     /locus_tag="PKN51_00585"
     CDS             48856..50706
                     /locus_tag="PKN51_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT83621.1"
                     /translation="MRILTAHLLPALALGTALLTSLPARATDATVARGLIVTLKAPSD
                     GGRESPQATRERLRAVAADAGLGGAQAPMPVGPGAHLLPFAAPLTGTAVQDAERRVRL
                     NPQVASVEPDVRLKRLATPNDPYYTDGIQWHLRTPAQGGAAALNLPPAWERTRGSAEQ
                     VVAVVDSGALYGHPDLAGKLLPGYDLISDLDTSNDGDGRDPDASDPGDWITSSEAGSG
                     RYPLCPAEDSSWHGSFIAGEIAAATHNGVGVAGVGWDTRVQPVRVSGKCGAWLSDVVE
                     GIRWAAGLPVSGVPTNPTPARVINVSFGGSSSCNSAYQTAIDDAGAAGSLVVVAAGNE
                     NGALTRPADCAGVLAVGAARQDGLKTYYSSYGPNVGIMAPGGPGAADTGPRLYSTSNA
                     GLRGPGEHIYDSKDGTSFASPLAAGVAALMLSVNPALTPAQLISRIQSGARPFPSSAA
                     YPTCRVGMPASSACNCTRETCGPGLLDADRALQLATSPSAVIAPIGSVPAGSTVTLDG
                     RGSAAVAGASIASYQWSQVSGSPVGIRDAGAPVASVSLPDARGDWVFRLLVTDSEGRA
                     ADNYVTVSTFGGDVDEGGGTTGPAWGAGLWALALGALWHRRTRRRTAATS"
     gene            complement(50909..51610)
                     /locus_tag="PKN51_00590"
     CDS             complement(50909..51610)
                     /locus_tag="PKN51_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNT83622.1"
                     /translation="MSDTPTPAHLAPTPAIDCDHPAVQALARQHAQGASERQRAVALT
                     HAVRDAFRYDPYRIDLSPAGMRASTVIAHGYGWCVPKAVLLAAAARAAGIPARLGFAD
                     VRNHLSTERMRQSMQTDLFIWHGYTELWLDGAWRKATPAFNLSLCQKFNLLPLDFDGV
                     HDSIYHPFDASGNRHMEYVRQRGSFDDLPLDRIVADFDRVYGPWLRRGTDSALGEGDF
                     LADVARETGAPSERA"
     gene            complement(51837..52718)
                     /locus_tag="PKN51_00595"
     CDS             complement(51837..52718)
                     /locus_tag="PKN51_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT83623.1"
                     /translation="MNDLDWNWLKAFLHTAQSGSLSAAARKLGLTQPTLSRQVAAIER
                     QMGVTLFERVGKSMVPTPTGLDLLEHARAMGNAAEALGLAANGRSQAVGGVVTVSASD
                     AVAAHLLPVIVRRLRAQEPGIAIEVISSNAVSDLLRREADIAIRHVKPEQPELIARLV
                     REAQALFYASESWVQTYGHPRTATDAAGLPFVGSDRNGRYLAYLRDHGLNLSEDSFSC
                     YADHSVTHWALVRQGLGIGAMMDEIARETPGMVRVLDDVAPVRFPIWLVTHRELRTSR
                     RIRVVFEALAEGLANGH"
     gene            52772..53545
                     /locus_tag="PKN51_00600"
     CDS             52772..53545
                     /locus_tag="PKN51_00600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT83624.1"
                     /translation="MDSIWGLLVANACDHHFGMSYGLHPMLLPPNTTVRDTSPSAASR
                     SASASKARFWDRIAPKYAADPIADMAGYETTLRRAQELLSPQQDVLEVGCGTGHTALR
                     LAPFTRRLMATDVSAEMIAIACLRQAAQPSPQLTFALADAEATDFGRATYDAVLAFNV
                     LHLTSNLGDALKPLVHALRPGGLLISKTPCIAEMHPWIPYLAVPLMRAIGKAPPVLCF
                     DAQRLQLTMVQQGLQIVCAERHGTRGKDTRVFIVARKPG"
     gene            53694..54263
                     /locus_tag="PKN51_00605"
     CDS             53694..54263
                     /locus_tag="PKN51_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83625.1"
                     /translation="MSLFSPTLHSTNRSGWSGNVLIAMAIVSLTACGGGSDGESGPPD
                     GGSGGTGPSVSIGALEGDWLQKGCVKAGAQSYKRTLRVRITGQTIVDYFEGVLTFRGN
                     DCAGASQAAGPTRTGTVTFARSEGSQVLTAHWGELRTVIGTRSGAIWTLQPQDLLCIL
                     GDEIPTIQPTLTAVSSSVATIPVDNCFGR"
     gene            54304..54729
                     /locus_tag="PKN51_00610"
     CDS             54304..54729
                     /locus_tag="PKN51_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83626.1"
                     /translation="MKPRMRLLSIAPMACVVAAQVLLPGCASTLDQAGLEQRTAQAIG
                     RSAGQFTITDQQEEIGGRVSYTVSTSEGATYRCYLYGATGFQKAMSFGQTPHSDAICT
                     DMVGSKPSSKHQAPATAPEQPGRNRAKACNALQRTAGRC"
     gene            54760..55614
                     /locus_tag="PKN51_00615"
     CDS             54760..55614
                     /locus_tag="PKN51_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT83627.1"
                     /translation="MFNLLKPDQWGKWPCVVLVLTLGLCGCTTVPGAAIETVDGRKVE
                     YAQSGQGTPVVVFENGLGATLDWWAKVWPDAVAEARSLAYNRAGYGHSDTSLAPRDGA
                     QVVDELRALLQARNLPPPYVLVGHSLGGLYMQLFARKFPDEVAALVLVDSTHPDQFKG
                     AGNPDQWPTWLKVVLHATTSETAKQELAALDATGEQVSGLPVAPSMPVWVLSALRATG
                     TPSALTDDADRKRSNIARLYPGSVQIWVNSGHAIPLEKPGAVVSAIRAAARATRASGA
                     ARMEPQDR"
     gene            55700..56011
                     /locus_tag="PKN51_00620"
     CDS             55700..56011
                     /locus_tag="PKN51_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83628.1"
                     /translation="MKLRTLAAGAGHDSQFEQYLRPSKPGMFLATTEQIVPWPELCAV
                     TEPHYPMPANGRPPAELERMLRTCLAQLRISPVDEPCEEALMDGTLVQARGLKAGTPV
                     P"
     gene            complement(56158..56838)
                     /locus_tag="PKN51_00625"
     CDS             complement(56158..56838)
                     /locus_tag="PKN51_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNT83629.1"
                     /translation="MRFEAILFDCDGVLVDSEPITHGVLRQQLAESGWDMPLAECMRH
                     FMGRTVRSQAALIEARTGRPLTDAWMARFYARRDAQLRQHLQPIDGAPDAVRAAHALT
                     GGRIACASGADRGKLVLQLAHTGLAPWFGPHVFSGHELPRSKPFPDVYLAAAAALAVP
                     PARCLVIEDTPTGIQAGVAAGATVWALCPQPEAAPALREAGAHALFGAMAELPRLWRA
                     GQAPAWAE"
     gene            complement(56955..58415)
                     /locus_tag="PKN51_00630"
     CDS             complement(56955..58415)
                     /locus_tag="PKN51_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNT83630.1"
                     /translation="MRRHVHVLRVLAWLIMGFSLAFLVPAAWTLVDARYRQAEIWLLA
                     GAGTAACGALLWAATRRFRRELSVKDGFLLVNLVWTVLPGFAAAPLLLTVPGISVTDA
                     YFEAMSGLTATGATALAGLDLLDPAVNMWRCFLQLIGGLGIMLLAVAVLPFLGLGGMQ
                     LFKAEMPGPMKDTRLTPRIAETARGLWGVYFVLSLACMLAYRWGGMSWDDAFMHMCTT
                     VGLGGLSSHDQSFGYWNSPALEWLAVLFMALSGISFARYFMVWRTRSVRPLWRDAEVR
                     AYVVVLLVSVAAVWLLLLREGKHVDAGHALRDAAFQVVSVATTTGYATTDYLLWPTIG
                     PVLMLLLGAIVSCAGSTGGGIKMVRVLILAKVTLHELVQLIHPRVVNPVTLGGQTVPA
                     RVIGAVIAYMLVYGAVLVGLSLLMLASGLDVVTAVSAIIACLNNIGPGMGKVGPAGNY
                     AVLTDFQIWLCSLAMMLGRLELLSVLVLFTPQFWRR"
     gene            complement(58439..59827)
                     /gene="trkA"
                     /locus_tag="PKN51_00635"
     CDS             complement(58439..59827)
                     /gene="trkA"
                     /locus_tag="PKN51_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNT83631.1"
                     /translation="MKILILGAGRVGQSVAETLASEANDITIIDPDPERLRGLEERLE
                     LRGVAGNGVQPSVLRHAGADDCDLFIACADLDATNLVACKVAHDVFGIPTTIARLRLP
                     DFRQDDRLLGPEGFAVSRVICPEASVTRTIQELIEHPEALQVLQFSQGRAHLVAARVA
                     RGSALDGRSIADFIAQVPEVPMRLVAVYRRDEETPCEADTRVLAGDEVFVLVAREHVQ
                     AALAAIGPREPPVRRVMIAGGGKVGLGVARSLIGQCEVKLIEKQRGRCRYLAGELPGD
                     MLVLQGDAADEELLAAEHVGDMDLFISLTSDDEDNIMSAMLAKRMGARRVMALINRRA
                     YAEMMQASPIDIIISPADAVIGELLAHVRRGAVAAVHSLRRGVAEALEGVAHGDARTS
                     RLVGRRIEQLALPAGARVGAIVRGEGDAGQVLVPHHDTVIEEGDHVIIFVPHKRLLPQ
                     VEKLFQVRATFF"
     gene            complement(59914..60336)
                     /locus_tag="PKN51_00640"
     CDS             complement(59914..60336)
                     /locus_tag="PKN51_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HNT83632.1"
                     /translation="MPRAHQADTPCPCGGRPPGTPYEACCGRYLDHWADTPAPDAEAL
                     MRSRYTAFVRGDADYLLSSWHASQRPATLALEPHIRWLGLEVRAHRVLDETHAEVEFV
                     ARSRVAGGRATRLHERSRFVRENVQGGWRWFYLDGDLS"
     gene            complement(60314..>61299)
                     /locus_tag="PKN51_00645"
     CDS             complement(60314..>61299)
                     /locus_tag="PKN51_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83633.1"
                     /translation="DLRRHQRRAEDHHHARAVALLKSDRVSSLLMEIPTNPEIPFFAY
                     GAFRPGELAFLKIREFVDRCEQDGVPGTLRIRDGLPIADLASTNSQIDGFLIFFREGT
                     QEEAHKKIVELEPDKQYEWKPIQSIGGQTANILAGLKPRKGSKELEEPWTGKNDPLFT
                     SALEVIDEVLDDIQNHQSTWDLKPLFRLEMAYLLLWTAIERYASLRYHLYRNANQKVE
                     EISTEMAFQNALRKYVKSEPDGNFRTVNRADNPEESYKLDPGNPKKSIKYYYQVRSNL
                     THRGKGIHADHHMMLSSLQELIAIFRETLAAAFAKSAQMLAENRGAASASS"
BASE COUNT         9335 a        21561 c        21081 g         9322 t
ORIGIN      
        1 cgtctggggc ggggctgacg cgaacccgct tacaacctgg tgcagtgttt cgcgccgtca
       61 ggcacttaca aaaaccagaa aaaagcttgc ggcaaggcgc ggctcgcaat aagtgcctga
      121 cggcgcgaaa cactgcacca gggtcagttg agcagccgat cgggctcggc gttgagccgc
      181 gccagggctt cgcgcgtggc gcgcacggtc agggtttcgt cctccaccgg caccagcagg
      241 ccggcctgca ggtacgaggc caggcgccag gtctgcacca ggtagctctt gcccgagccg
      301 tcgccgaaca ggtgcagctg gccacgcttg ctgcgccaca cgtactgcac ctgggtcacg
      361 ctgcccaggt agtcgagcgt gaaccaggcc ccgagctcca gctcgccggc ccagtgcagc
      421 atgccctcgc cgggctgtga gccgccgctg gcaatcacct ccagcgcgtc gccttcggcg
      481 ccgaacatca gctcgatcac gcccgggtcg agcagcacgt cgccctgggc gtcgtcgctg
      541 accacgtctt ccagaccggc caggttgcgc gacagctcgg ccagccgctc cgccgacagg
      601 ccctcgccgc gcgagacgaa ggcctgcacc accgcgtcgt tcagcacctt gatgtgggca
      661 tcctggtccg aggtcgacag cgccaggcct tccatgccct cgcgcagggc gtgcaacagc
      721 gtcggcagct gctgcaccac gcggccgcgt tcgctgcggt cggccttggg gctgaccgcc
      781 cacagcagat cggccgccgc ctgcttgaag cgcagcgtgt cgggcgcctg cggcccgcgc
      841 cgcaccgcgg ccatggccag cacctcgcac cagacgcgga acaggaactc gcgcaccggc
      901 tcgggcacgg gcaccggctc cagcatgcgc cgcagctcga tggtgtactg cacggtcagc
      961 gcttccttct gctcgatctg ctgcgccagc gtcgccatct gctgtgcgga gccgccttcg
     1021 gtcagtgagc gaccgagaaa cttcttgaac tcttccagca ccagctggta gacgcggcgg
     1081 ccggtttcag ggtattgctc gatcacctgc acgatgcgct tgatctcgcg ctccaggcgg
     1141 ctgccgccga cctgcgcggc gtcgaagccc agcgcgcagg cacccatgcg gtcgatcaac
     1201 tggcgcgccg ggtgctggat ggaggcgaag aagtccggct cggccaacgc cagccgcaac
     1261 accggaatct gcaggcgcgc gaaccagacg cggatcgccg ccgcgatgcg ctcttcggcc
     1321 aggatggcct ggaacatcag ggccacgatc tcgatggtgg ccttctcgct cggtcgctcc
     1381 gccttggcct tcagctcggt ggctcgctgg cgcagacgcg tcgccaccgc ctcgacactg
     1441 gccacgggtt gcacgatggt cacgtcggcg tcgctgcggt ccagcaactc ggtgactgcg
     1501 aaaggcgccg cctcggccat ggcgcgagcc aacggcggcg aaggtggctc tgccggcgcc
     1561 gccaggtcga agttcgccaa ctgatcggtc agcagccgtc gcagctgccc cagcacgcca
     1621 tgcgctcgtt gccgtacgcg cgtcatgggc gagatggccg tcatcatgcg ggtttcctgg
     1681 gctgcgcgcg ccagcgcgga gtgccgcacg tcgggtgtct gggtgtcatc cgcggcattg
     1741 gcccggccat gggtggtaaa cgcgtcgcca cgggccgacc ggccatcgcc gaacagtccg
     1801 gcaccgcctg agcgcgcgcc cgattcccgg gaagcggcat tttccgcggg tccgggcgct
     1861 gcgcgatcgc gcgcacccgc aaccgccgac gctcgagcac ctgcgacagg cccggcatcg
     1921 ccatcaccac gccgcaccag cgccttcagg tcgatttcgg ccagcacccc gcgctcgatc
     1981 aggaaagcat tggcctgccc gtagagtggc accaagcgtt cgatcattgc cgcctggatc
     2041 tccgggtgaa ccagtaccca catcggacga tccaggccgg cgtcccgcca ggccttgagc
     2101 aactgctgtg caaaggtgtc gggccgcagc acgtcgcgcg ccggcaggtc ggtgctgcgc
     2161 tcgagcgcct gcatgcgcag ccgcagatcg ttgaattcgg cgccagcagc gtcctgcacg
     2221 gccgccccca gtcgcgaggc ggcgatcttg cgctcgacac tgtcgtcgtc caccagaccc
     2281 agatcacccg gcagactgcg acgcgcggcg cggctgtccg tcatctgcgt cagtgcatcg
     2341 cgccagtggc gccgtgccgc cgtcacccag gcggtgcgcg cacgccgaaa cgctgcctcg
     2401 gcctccatgg cacgctgcat gtcggccagc gtcgcgccgc ggatcccgcc ttgcgcctgg
     2461 cccgtcagat gagtctggat tcgctcaccc agctcgatga cggctgcgtc gagcacctgc
     2521 aggaagcgct cgcgcacctg ctgcgcgagg cgatgaggcg acggtggtgg tggcgtcgaa
     2581 accatgggag cggttgtggc ccctcagtat ggcacgcccg gcgtggaatc cagggccgct
     2641 gtcttacacc accgcgccgg cgttcgggtc accggggtcg ccatccttgc ggccggtctt
     2701 gaccaggtcc tcgcgccgga tgcccagcca catggcgatg gcggcggcca cgaacaccga
     2761 ggaatagatg ccgaacagga tgccgatggt cagtgccacg gcgaagtaat gcagcgtctc
     2821 gccgccgaac agcaacatcg acagcaccat gatctgcgtc gagccgtggg tgatgatggt
     2881 gcggctgatg ttggcggtga tggagttgtt gatgatctcc accgtgctca tcttgcggta
     2941 gcggcggaag ttctcgcgga tgcggtcgaa gatcaccacc gattcgttga ccgaatagcc
     3001 cagcacggcc aggatcgccg ccagcaccga gagcgagaac tcccactgga acagggcgaa
     3061 gaagcccaga atgatcacca cgtcgtgcag gttggcgatg atgccggcca cggcgtactt
     3121 ccattcgaag cgcatcgaca ggtagatcat gatgccgacc accacgaagg ccagcgcgta
     3181 caggccgtcg cgcgccagct cgtcgccgac ctgcgggccg acgaattcgg tgcggcgcaa
     3241 ctgcacgtcg gggctggcgc tgcgcagcgc ggccagcacc tgctcgctct gctgggccga
     3301 gctcttgccc tgctgcaccg gcagccggat gatgacgtcg cgcgcggtgc cgaagttctg
     3361 tacctgcacg tcgccgtaac ccagcgccgc caccgtgcca cgcacctgcg ccagatcggc
     3421 cggctggctg taggccacct ccatcaccgt gccgccggtg aactcgaccg acaggtgcag
     3481 gccacgcgtg aacagaaaca ccaccgccag caggaaggtg acgatcgata tggcgttgaa
     3541 aagccgcgca tagcgcatga acgggatgtc ccgtccgatc ctgaagaatt ccatctgcct
     3601 gttccttgct tgtctgacgg cggttgcggc gcgctcacac cttctcgggg gcgggtcgcc
     3661 acacggtgcc gatcgacacc gacttgagct tcttctgccg cccataccac aggttgacca
     3721 ggccgcgcga gaaaaacacc gccgagtaca tcgaggtcag gatgccgatg cagtgcacca
     3781 cggcaaagcc gcgcaccggg ccggtgccga acgccagcag cgccaggccg gcgatcagcg
     3841 aggtgacgtt ggagtccagg atggtggccc aggcgcggtc gtagccggca tggatggccg
     3901 cctgcggcga ggcgccaccg cgcagctcct cgcgcacgcg ttcgttgatc agcacgttgg
     3961 cgtcgatcgc catgcccagc gtcagcgcca ccgccgccat gcccggcagc gtcagcgtgg
     4021 cctgcagcat cgacagaatg gccaccagca gcagcaggtt gacgctcagc gcaacggccg
     4081 aaaacacgcc gaacagcatg tagtaggcga tcatgaacac cacgatggcc agaaagcccc
     4141 acatcacgct gtggaaaccc ttgctgatgt tgtcggcgcc caggctgggg ccgatggtgc
     4201 gctcctcgat gatctccatc ggcgcggcca gcgaaccggc gcgcagcagc agcgccaggt
     4261 cgttggcctc gacgaccgac atggagcccg aaatctgaaa acgcgtaccc agctcggcct
     4321 ggatcaccgg cgccgtcagc acctcgccct tgcccttctc gaacagcacg atggccatgc
     4381 gcttcttcag gttctcgcgg ctgacgtcgc gcatcacacg cccgcccttg gcgtccaccg
     4441 tcaggtcgac cttgggctgc tgggtctgct gatcgaagcc gggctgggcg tcggtcaggc
     4501 tgtcgccggt caggatcacc tggcgcttga cgatcaccgg gcggccgtcg cgctcgagga
     4561 atttttcgga gccgaacggc accgggccgc cggtttcggc ggcacggcct tcggcgcttt
     4621 cgtcgaccag ccgcatctcc agcgtggcgg tgcgccccag gatgtccttg gccttggccg
     4681 tgtcctgcac gccgggcagc tgcaccacca cgcggtccac cccttgctgg gcgatcaccg
     4741 gttcggccac gcccagctcg ttgacgcggt tgtgcagcgt ggtgatgttc tgcttgaccg
     4801 catcggcctg cacgcgctgc agcgcctgcg gcttgaagct ggccaccagc cgcggctcgg
     4861 tgccctgggt cggctccacc tgcaggtcgg gctgggtgtc gacgatgacg cggcgcgccg
     4921 cctccagcgt gtcggcatcg cgcgcgcgga tctcgatctg gttgccttcg cggctgatgc
     4981 cgccgtggcg cacgtccttc tcgcgcagcg ccacccggat gtcgttggcc agcgtgtcca
     5041 ggcgcttggt gatggccgtg cgcatgtcca cctgcagcat gaagtgcacg ccaccgcgca
     5101 gatccagccc caggtacatg ggctgggcat tgatggccgc cagccaggcc ggcgaacgcg
     5161 acaccaggtt gagcgcaatg atgtgggccg ggtcggccgc gtcggggttg agtgcgcgct
     5221 cgatgacgtc cttggccttg agctgggtgt cgggcgtgtc gaagcgcgcc cacaccgagc
     5281 tgccttccag cgtcaggcgc tggggcgtca ggccggccgc ctgcagcgcg ccttccacac
     5341 gcgacagcgt gccgccatcg atccgggtgg tggccttgcc ggccgacacc tgcaccgccg
     5401 gcgcctcgcc gaagaagttg ggcagggtgt agagcgccgc caccaccagg gtgatcagga
     5461 tgacgatgta tttccagagg gggtagcggt tcatggcgac ggcaggatgg gggcggccag
     5521 atgcgcgccg gcgcgagggc cggcgccggc gtccgggctt acttcagggt tcccttgggc
     5581 agcacctgca cgatggcgct gcgctgcatc tgcacctcga cgccgctggc gatctgcagg
     5641 ccgatctggc cttccgacag cgccgtgacg cgccccatca ggccgccggc ggtcaccacc
     5701 tcgtcgccct tggccagcgc gtcgatcatg gcgcggtgtt ccttctgctt cttcatctgc
     5761 gggcggatca tgatgaagta cagcaccacg aacatcagca ccagcggcag catgctggtg
     5821 aaggtggaag cgaggtcggc cccgggggcg gcggggacgg cttgggcgta ggcgttggag
     5881 atgaacaccg gcaggctcct gaaatgggcg gtgatggatg gatcgcgcgc gtggcgcgca
     5941 actgcgcgcc cggcggggcg cgcgcaggca accgtgcatt gtaaggcgcg ctggcgcgcg
     6001 gggccgcgct gggggcccaa cgcgcagcct tcgaatcatg gatcgataac tatcaatatc
     6061 atagctgctg gcgcagactg ggcggtcgcg aaacccatat ttcgcccgaa tttcaggcga
     6121 tctgccgcgc cggcgcgacg ggcggctggc ggtactgcgc ccgcagctcg tcccagcgcg
     6181 cacgcgccgc cttcaggtgg cgctccttga cgtggccgaa gcccttgatg gcctgcggaa
     6241 tgcgcgcgat ctcgaccgcg atggcgtggt tgtcggcgtt cagcgtggcc agcagctcgt
     6301 cgatgctggc gcggtactgc tcgatcagcg cgcgctcagt ctggcgctcg tcggtcttgc
     6361 cgaacagatc cagcgcgccg ccgcgcaggc ccttgagccc ggccagcaga cggaacaccc
     6421 gccccatgcc ggggccgtat ttcttcttga ccagctcgcc gcgctcgttg cgctgggccg
     6481 tcatgggcgg cgccaggtgg tagtgcagct tgtagtcgcc ttcgaacatg gcgtcgatgc
     6541 gcgccaggaa ggccgggtcg ctgtgcaggc gcgccacctc gtactcgtcc ttgtaggcca
     6601 tcagcttgaa caggccctgc gccaccgcct cggtcaactg ggttctgccc agcgccgcct
     6661 cctgttgctg cacccgggcc acgaaggcgc ggtagcgctc ggcataggcg gcgttctggt
     6721 agccggtgag gaattcgacg cgccgggcga cgatggattc cagcgtctcg cgcggcttga
     6781 acgccaccac ctgctcgccg ccggcgccgg ccagcgcctg ctcgacgcgc gcgccgtcgt
     6841 gcgccgcctg gcggccccat tcgaaggccg ccttgttgtt gtcgacctgc acgttgttca
     6901 gttcgatggc gcgcagcagc gactcacgcc tgagcggcac ccagccgcgc tgccaggcgt
     6961 agcccaggat catggggttg acgtagatgg tgtcgcccag cagccggccg gccaggcggt
     7021 cggcgtcgaa gctggccagg ccctgttcgc ccacggcgcg ggcgatgtcg gcggcgcagc
     7081 gcgcctgcgg gttctgccag ccgccgtcct tgacgaaggc cgccgtgggc gtggcgtggg
     7141 cgttcagcgc cacgtgcgtg cgcccctcgc gcatgcgggc cacggtttcc ttgttggcgg
     7201 tgacgatcgg gtcgcagccg atgatgaggt cggccgccgc cgcgctcacc cgcgtggtgc
     7261 ggatatcgtc ctgcgtggcg ccgatcagca catggctcca ggtggcgccg cccttttgcg
     7321 ccaggccggc ggcgtcctgg gtgacgatgc ccttgccttc caggtgcgcc gccatgccca
     7381 gcaactgacc gatggtgatg acgccggtgc cgcccacgcc ggccaccacg atgccccagg
     7441 cgccgccggc ctcgggctgg ggcaacggcg gctcgggcag cgcaccgagc tcgaacggcg
     7501 acacgctggc gcgcgccttg gccttcttgc gcagctgccc gccttccacc gtgacgaagc
     7561 tggggcaaaa gcccttgacg caggagatgt ccttgttgca ggtgctctgg ttgatggtgc
     7621 gcttgcggcc gaattcggtg tccagcggct ccaccgacag acagttgctc tgcacgctgc
     7681 agtcgccgca gccttcgcac accgcctcgt tgatgagcac gcgcaccgcc gggtccacca
     7741 tggtgccgcg cttgcggcgg cggcgctttt cggtggcgca ggtctggtcg tagatgatga
     7801 cggtggtgcc ggcgatctcg cggaactcgc gctggatggc gtccagccgg tcgcggtgct
     7861 gcacgtcgat gccctcgggc aggccgtgct ggcgcgcctg ggcgtatttt tcgggctcgt
     7921 cggtgacgac ggtgatcttc accgcgccct cggcacgcat gttctgcgcg atctggatca
     7981 ccgacagacc ctcggggcgc tcgccgatcg gctggccgcc ggtcatggcg acggcgtcgt
     8041 tgtagaggat cttgtaggtg atgttgacgc ccgcggccac cgactggcgc accgccagcg
     8101 agccgctgtg gtagtaggtg ccgtcgccca ggttggcgaa cacgtgctgt tcgttgctga
     8161 acggctgctg gccgacccag ggcacgccct cgccgcccat ctgcgtgaag ccgacggtgg
     8221 cgcggtccat ccagatcgac atgaagtggc agccgatgcc ggccatggcg cgcgagccct
     8281 cgggcacgcg ggtgctggtg ttgtgcgggc agcccgagca gaaccagggc tgacggtcgg
     8341 cgccgctgct gctggcctgc agcacctgca ggttgcgctc gccggcgtcc aggatggcca
     8401 gctgacggtc gatgcgggcg gtggtgtcgg cgtcgatgcc gagctttttc aggcgctcgg
     8461 cgatggcgcg cgcgatcagc gccggcgaca ggtcggcggt ggcgcgcagc agcacgttgg
     8521 ccgtggggtt gtcgagcgac cattcgccgc cgctgtagtc gcccgcggcg ccctcgcggt
     8581 acttgcccag cacgttgggc cgcacgtcgg cgcgccagtt gtagagctgc tccttgagct
     8641 ggtattcgat gacctggcgc ttttcctcga tcaccaggat ctcgcgcagg ccggcggcga
     8701 actggcgcgt gatctgcgcc tccagcggcc acaccaccgc caccttgtgc aggcggatgc
     8761 cgagctggcg gcaggcggcg tcgtccagcc ccaggtccag cagcgcctgg cgggtgtcgt
     8821 tgtaggcctt gccgctggcg atcaggccca ggcggtcgtt cggcccttcg atgacgttgc
     8881 ggttcaggcg gttggcgcgc acataggcca gggccgcgta ccacttggtg ttgaacaggc
     8941 gctcctcctg cggcagcgcg gcgtcgggcc agcggatgtg cacgccgccg ggcggcatgt
     9001 cgaagtcggt cggcagcgcg atctgcaccc gttcggggtc gatcagcgcg ctggcgctgg
     9061 attcgacgat ctcctggatc gtcttcatgc cggcccacac gccggcgtag cggctcatgg
     9121 cgaaggcgtg cacgcccaga tccaggatgt cctgcacgct ggccggaaag aacaccggaa
     9181 agccgcaggc cttgaagatg tggtcgctct ggtgcgcggc ggtgctgctc ttggccaggt
     9241 gatcgtcgcc ggcgacggca atcacgccgc cccagggcgt ggtgccggcc atgttggcgt
     9301 gcttgaacac gtcgacgctg cggtccaccc ccggcccctt gccgtaccag atgccgaaca
     9361 cgccgtcgaa gcgctggctg ccgggcggcg caaagcccag ctgctgcgtg ccccagacgg
     9421 cggtggcggc cagctcttcg ttgacgccgg gctggaacac gatgtgctgg ccctgcagat
     9481 gcttgcgggc ggcccacagc gcctggtcgt agccgcccag gggcgagccg cggtagccgc
     9541 tgatgaagcc ggcggtgttc ttgccggcga gcgcatcgcg ctggcgctgc agcatcggca
     9601 gcttgaccag cgcctggatg ccgctcatga aggcgcggcc gtgctccagg ctgtatttgt
     9661 cgtccagcgt gacggtttcc agcgccttgc ggatgtgttc gggcagcggg gcgttcatcg
     9721 gggtggtctc ctgtgtcggg ccgcctggcc gatggccggc agccgcctgt gtgtcgggtc
     9781 ggcaaagtgt aggtcggctg acgcgacagg tgctttcttt atgtgccgcg atccacggtg
     9841 gtcaggaaag attctttcta gaatccaccc atcatggaaa cacttgacgc ccacgatctg
     9901 gccatcctgg aacaactgca ggccgacggc cggctcacca acgccgaact tgcgcagcgc
     9961 gtcggcctgt cggccgcacc ctgctggcgg cgcgtgcgcg cgctggagca ggctggcttc
    10021 atccgcggtt accgcgccga aatcgatcgc cacaagctcg gcctgggcgt gctggccttc
    10081 gtgcggctgg atgcggccca ggtcagcggc gcggcgctgc gggcgctgga ggcggcgatc
    10141 cgcacccgcc ccgaaatcgt cagctgccac tacatcagcg gcaccggcac cttcgagctg
    10201 caggtggtga gcaccgacct ggaaggcttc agccgcttcg cgcgcgaggt gctgctgaac
    10261 ctgcccaacg tgaaggacat gcacaccagc ttctcgctgg gcgaggtcaa ggcggccggc
    10321 gcgctgccgc tggagcacct cagggccaag ccgcgccgcg gcggttagtt gcccgccctc
    10381 aacccggccc gttgcgaccg tcgccctgcc ggatgttggt ctgcaccggg gcgtcggggt
    10441 cgggtcgcgg cggcggcggg cgcgggccga ggcgcgggct ggcgcgcacg gccgccgccg
    10501 gggcgtcggc gccgggcagg tgctggcagt acaccggctc ggggatgccg gtgtagtcca
    10561 gccgcaccag gcgcgcgtcc agaccgaggc cgaagcgcgc ccgcaccgtg gggtcggcat
    10621 agtgcagcgc gtcgccttcg gcgccgggca cgcgctcgag cacggcgtgg ccgcgcaggc
    10681 cttcgagcag ggccatgtcc tggtacagcc gggcctcgaa gcgcagctcg tgcgggcact
    10741 ggtaggccag gcgtggcgca tcctgccagc tcgcgcagcc gcccagcgcg gccagtgcca
    10801 ggccgccggc gagccgcctg aatgctataa ataacgtagc ttctggcgca gatcgggcgg
    10861 gcggcgcagc catttttgct ttgaacatcg aggcggggcg cgcggcgccc gcccaggcgg
    10921 cgcggcggcc cgcttcgggc gcgcgggcgc ggctcaccgg ggccacagca cccacaggcg
    10981 cagcggctgc ttcatgcggc cggccaattc gcccgcctcg gcgccccagc cggcaaagta
    11041 gtcggcgcgc acggcgccga cgatggcgct gccggtgtcc tgcgccagca ccaggcgctg
    11101 cagctgcgcc accggcccct gcgaggacag ccacaccggc gtgccgtagg gaatgctctg
    11161 cgggtcgacg gcgatcgagc gccccggcgt cagcgccaca ccctgcgcgc cgcgcgggcc
    11221 gaactgcgcc tccagcgccg acagcggctc ctcgcggaag aacaccacgc gcgggttgct
    11281 ccacagaaag ccctgcaggt gctgcggctg cgccgccagc gcggcgcgga tgccgggcca
    11341 ggtggcgtcg ccgcgcagca gcccctgctc gatcagccac ttgccggggc tgcggtaagg
    11401 gtgttcgttg gtgccggcaa aggccagccg cagcaggcgc tggctgccgt cggcctcctg
    11461 cacgcgcacc cggcccgagc cctggatctg caggatcagc gcgtccagcg gatcggccag
    11521 ccagaccagc tcgtggccgg ccagctgggc gcgcgcctcg ggcagggtgt cgagctcctg
    11581 gcgcgtgtac cagggcttgc gctggctggc gccgggcggg gcgcggtaca ccggcgcgcc
    11641 aaagcccggg cgcgccacgc gcgaggcttc gtacaccggc tcgaagtagc tggtcagcag
    11701 gccgtcgccg gccggcgcgc cggccagggg ctcgacgcgg tagggctgca ggcgcgcgcg
    11761 catccagtcg cgctgctgct gcgtgctgcc cagcgccagg cgccgcacct cgctgcacag
    11821 acgggcaaag gtggggccgg ggcgctcgca gctcttgagc caggcgttcc aggcggcggc
    11881 ggtgtcgtcc tgctcgaagc cgggcagctc ggcccagcgc accggcaccc agcggctgcg
    11941 cgcgcgctcg atgggcgggc ccagcggcgc cgtgtcggcc accgggccgg cggggggcag
    12001 accgggctcg gccggcggca gcggggcggg cgccggcggc gggcgcagcg cgcaggcggc
    12061 cagcagagcg gccatggccg ccgccgccag aatccgtgat actcgttgca tgaccctgat
    12121 tctgctggaa gcgctgggcg ccctgttgct gttcgtgctg ctggtgtggt ggaccatgtt
    12181 cgccggccgc aagaagggcg agctgcccga gccggccgac gacgatgccg aatgaaattg
    12241 gcctctggcg cccgcctggc aagcgccgat agctatattt gttgtagcaa ctgggccagc
    12301 tcgacggccg atttgacgtc catcttctcg aacacccggg cgcggtgcac ctccaccgtg
    12361 cgcacgctga tgcccagctc gtcggcgatc agcttgttgg ggcgcccgct ggccacccgc
    12421 tgcagcacct cgcgctcgcg ctcggtcagc tcgtccaggc gcgcgcgcag cgcacgctgc
    12481 gcaccgagct gcgccacgcg ctcgcgcgac accgccagcg cgcgctcgac gcgatcgacc
    12541 agcgcgttgt cggaaaacgg cttctcgcag aagtcgaagg cgccgcgctt gaccgcctcc
    12601 accgccgtcg ccacgctggc gtgaccggtc agaaagatca ccggccaggc ggtggcgatg
    12661 ccgcgctcca gcaggcgatc gaacagcgcc agcccgctgg tgccgggcat gcgcacgtcg
    12721 agcaacaggc aggccggcgt ctgcggcggc gcctcgccca gcgcctgctc gaaggcctgt
    12781 gcgctgtcga agccttccga gcacagccgg cgcgagcgca gcagccaggc cagcgccgcg
    12841 cgcacctcgg catcgtcgtc gacgatgtag accatggcat cggcagcgga catgcggcgg
    12901 attctatgga ttcggaatcg tgaccatcgc gccgttcgct ctgagcctgt ccaagggctg
    12961 cgacaggctc ggcccgaacg gtgctcaaga catcccggag gccgaatcca caggaggctg
    13021 tcgaagctcg gcttggctgg gcctggccgg ctggccgtaa catccggcct cgttcatctg
    13081 ggagagttct gcatgtccac cccgttcctg cgccgtctcg tcatgccggc caccttgtgc
    13141 ctggccgcgg ccctgagcgc ctgcggcggc ggcggcgacg acgacgccac cccgccgccg
    13201 cctgcgcccc cgggtcaggc cagcggcagc ctgctgtcga gcgcgccggt caacacctgg
    13261 tcggccgagc agatcgcgca ggcgctgtcc gaccccgaca gcaaggtgca gagcggcgtc
    13321 acgccgcgct acgcggtgcg ctcgcaccgt gtgacctacg tcagcagcga caagcacggc
    13381 cagccggtca cggcgtcggg gctgatcagc gtgcccctca agccgcccgg cgcccgcagc
    13441 ccggtgctga gctaccagca cgccaccacc ttccacgacg accaggcgcc cagcaacaag
    13501 gtggaggcgg tggagccgcc gatcgtgctg gcctcgctgg gctacatcgt gctgtcggcc
    13561 gattacgtgg gtttcggtgc cgccaagggc ctggagcacc cctacctgac ggccgcgccg
    13621 agcgccgcgg tggtgctgga cctgctgcac gccggccaga cctggcgcca gcagcaggcc
    13681 gtggccgaca acggccagct gttcctggcc ggctattcgg aaggcggcta cgtcaccctg
    13741 gcggcgcagc gggccatcga aaccacgggc gacgcgctgc gccagcagct gcaggccagc
    13801 gtgccgggcg ccggccccta cgacgtgcag gcgacgctgg acgcgcaggt cgaggccgtg
    13861 cgcgcctaca acagcacgct gggcaagctg ttcgagccgc atcgcctgtc gcaggcgccc
    13921 gaatgggtgc gcaacgaact gcgccgcctg ctgctgcgcc agatggtgcc ggacgacgcc
    13981 gacgtgcgct accagacgct gttcctcgac cgctacatgg ccgacgacca ggccgccatc
    14041 gccgccgagc acagcgtgca cctgggctgg gcgcccgccg tgccggtgta cctgttccac
    14101 ggccgcgacg accggaccgt gccctacgcg gcggccactt cggcgctgaa caccctgcgc
    14161 gccgccggtg cgcgcgacat cagcctgacc gactgcacca caccggacta cggccacctg
    14221 aaatgcgtgc cggaatactt ccgcttcgcg gtcgagcgca tggggcggct ggcgcgcgac
    14281 ctgtgacgac ccttgaacct atggattctt gggtgagcac gctacgcgcc caccggcacc
    14341 gggtcgaccg gcagcgccag cccctccttg atttcctcca tcaccacgta gctgttgctc
    14401 tgcgccggca ccggcatctt gcgcaggatc tggcccagca ggtcgcggta ctcgcgcatc
    14461 gccgacaggc gcgccttgac caggtagtcg aaggtgcccg acaccagatg gcattccagc
    14521 acgcgcggct ccagcagcag ctcgtcgcgc acctggcgga acacctcggc cgacttggac
    14581 gacagcgtca gctccacgaa caccagcagc ccgcgcccca gcgcctcggg gttgacgcgc
    14641 gcgtggtagc cctggatcac gccgctgcgc tccagccgcc gcacccgctc ggcacagggc
    14701 gaggtggaca gccccacctg ctgcgccagc tcggtcatcg agatgcggcc atcgcgctgc
    14761 agctggtcga ggattttgcg gtcgatgcga tcgatggagc tcatttcact gccaaacgat
    14821 attcattcag tgattttcac tgactcagtg gccgcgagca agtcatttca gtgacttttg
    14881 tgcccctccc cgccctagac ttccggcatt cccacaggat catggagaca ggcatgaagg
    14941 tcatcgttct gggcggcggc gtgatcggcg tcagcacggc gtactacctg gcgcgcgccg
    15001 gcgccgaagt caccctgctg gagcgccagg acgacgtggc gctggaaacc agttttgcca
    15061 acgccggtca ggtgtcgccc ggctattcca cgccctgggc cgcgcccggc attccgctca
    15121 aggccgtcaa gtggctgttc cagaagcacg cgccgctgtc gatccgcccg gacggcacgc
    15181 tgttccagtt gcgctggatg gcccacatgc tgcgccactg caccagcgcg cgctacgccg
    15241 tcaacaagga gcgcatgacg cgcctggccg aatacagccg cgactgcctg cgccagctgc
    15301 gcgccgacac tggcatcgag tacgagcagc gcagcctggg cacgctgcag gtgttccgca
    15361 ccgcggcgca gttgcaggcg gcccggcgcg acaccgccgt gctgcaggag tgtggcgtgc
    15421 cctaccgcct gatgaccgac gtagccgagc tggccgaata cgaaccggcg ctggcgcgcg
    15481 cgcgcggcct ggtcggcggc ctgcagctgc cgggcgacga aaccggcgac tgccggcgct
    15541 tcaccaccgc gctggccgaa caggcgcgcg ccctgggcgt gcggttccgc ctgggcgtgc
    15601 agatcgccga gctgctgccg cgcggcggtc agatcggcgg cgtgcggctg gccagcggcg
    15661 aggtgctgag cgccgaccgc tacgtgctgg ccctgggcag ctgctcgcgg gcgctgctgg
    15721 cgccgctggg gctcgatctg ccggtgtacc cggtcaaggg ctattcgctc accgtgccgc
    15781 tgctcgaccc ggccaacgcg ccgcgctcca ccgtgatgga cgaaacctac aaggtcgcca
    15841 tcacccgctt cgaccagcgc atccgcgtcg gcggcatggc cgagctgggc ggcttcgacc
    15901 tgcgcctgaa cccgcgccgg cgcgaaacgc tggagctggt gctgggcaac ctgttcaccg
    15961 gcggcgacat cccgcgcgcc gagttctgga ccggcctgcg ccccatgacg cccgacagca
    16021 cgccggtggt cggcgccacg cgctacgcca acctgctgct caacaccggc cacggcaccc
    16081 tgggctggac catggcggcc ggctcgggca agctggtggc cgacctgacg ctggggcgtc
    16141 agagcgacat ctcgaccgcc gggctgggcc tggagcgcta cggccgcagc caggccggcc
    16201 cgcgctcgct gccgcggcac gtgccggcct gacggcacgg gctgccgcgt cccgcgccat
    16261 ccgcgggcgg ggctggacgc aaccatccgg agcgtcgcac gcccctgcgg cgcatcgacc
    16321 atcgcgcgcg tgtcggccag cacgccaagg cgcggccggc ctccgtggtt ggccccgggt
    16381 ttgatggcaa atcaggctct ggcgctttac tggcaagcgc tggcagctat caaaacagga
    16441 gtcttcggac ctcgaacgcc aggagccatc cccgccaacg acccatgacc catgggtcat
    16501 cgacgcgcag cgaggtcatt tcgtcatccg tcaggcctgg aaccacccgc ccgcttaggc
    16561 cacttcggcc agggcgtcgc ccagcgcgtt caccaggcgc tcgatctcgg ccttttcgct
    16621 gatgaagggc ggcgccagct ggatggtgtc gccgccgtag cgcacgtaaa agcccttctt
    16681 ccagcaggcc atggcgatgt cgtacgggcg tcgcgccggc tcgcccgcca ccggggcgat
    16741 ggtgaagccg gcggccaggc cgtagttgcg gatgtccagc acatggcggg cgccccggag
    16801 gctgtgcacg gcggcttcga aatgcgcgct cagggccttc acgcggccgg ggccgtcttc
    16861 gcgctcgagc agctccagcg ccgccagacc ggcggcgcag gccaccgggt gcgcgctgta
    16921 ggtgtagccg tgggcgaact ccagcatgta gtcggggccg ccggcggcca tgaaggtgtc
    16981 gtagatcgcc cggctggcca ccacgccacc cagcggctgc acgccgttgg tgacctgttt
    17041 ggcaaaggtc atgaggtcgg gcaccacgcc aaaggcgtcg ctgccagtca tggcgccgca
    17101 gcggccgaag ccggtgatga cctcgtcgaa gatcagcagg atgtcgtgct gggtgcagat
    17161 ctcgcgcagg cgctgcaggt agccgaccgg cggaatcacc accccggccg agccggaaaa
    17221 cggttcgacg atgacggcgg cgatgttgct ggcgtcgtgc agggcgatca gctccagcag
    17281 gcgatcggcc aacgccaccc caccctcggc cggctggccc ttggcgaagg tgccggccgg
    17341 cggctgggtg tgcggcaggt gatcggcctc gatgccctga ccgaacagct tgcggttgcc
    17401 caccatgccg ccgaccgaga tgccgccgaa gttgaccccg tggtagccct tttcgcgccc
    17461 gatcaggcgc gtcttgccgc cctggccccg ggcgcgccag taggcgcgcg ccatcttcag
    17521 cgcggtgtcg gcggcttccg agccgctggc ggtgaagaac acgtgatcca gcccctgggg
    17581 cgtgcgctgc ttgatggcgt tggccagctc gaacgacagc gggtggccga actggaacgc
    17641 cggcgcgtag tccagctgcg cggcctggcg gcccacggcc tcggcgatct cgcgccggcc
    17701 atggcccagg ccactgcacc acagtccgga caggccgtcg aacacctggc ggccgtcggc
    17761 gtcggtgaaa taggccccct gggccgccac gatcaggcgc gggtcgcgct tgaactggcg
    17821 gttgccggta aagggcatcc agtgcgcctc gagccaggcg gcgtcgcggc gggtggcggc
    17881 gggggcagga acggtggcag tgacgctcat gggggtctcc tcggatcggc agcttgggcc
    17941 cggacgggcg gaacaacgcc attgtcggcg ccgtcgttag ccgtttaaat acgcaaatat
    18001 cgaagttcag ttgccggatc atgcaagtca atcccacccc tgcccgcagc cgcgccgtgc
    18061 tgggtcaggt gagcgacatg gacctgcgcc tgctgcaggt gttcaaggcg gtggtcgaat
    18121 gcggcggcct gtcggcggcc gagctggagc tcaacatcgg caccagcacc gtcagccgcc
    18181 acgtcaagga cctggaaacc cgcctgggcc tgacgctgtg ccggcgcggg cgcggcggct
    18241 ttgccgtcac gcccgagggc cagcgcgtgt acgacgagac gcggcgcctg ctggcggcgg
    18301 tggacggctt tcgcgccagc gtcgacgaca tccaccagcg catgggcggc acgctggcgg
    18361 tggcgctgtt cgacaagacc gtcagcaacc cgcaggcgcg catccacgcg gccatcgccg
    18421 acttcacgca gcaggcgccg gacgtggcgc tgcacctgca cgtgggcagc atccagacca
    18481 tcgaacgcgg ggtgatggac ggcagcttcg ccgtcggcgt gattccggcg caccgcgcct
    18541 cggccagcct gcgctaccag cggctgttcg gcgagaccat gcacctgtac tgcgcccggc
    18601 cccatgcgct cttcgattca gagcattcgg cgctggactg gcctgccctg cgggcgcgcc
    18661 aggactttgc cggcctgggc taccactcac ccaacatgga gctggcgcac ggccagcggc
    18721 tgcggcgcgg cgccaccgcc tacgaccagg aggcgatcgc cacgctgatc ctgtcgggcc
    18781 gcttcatcgg ttttctgccc gaccactacg ccgccgcctt cgtgcagcgc ggccagctgc
    18841 gcgccgtggc gcccgagcgg ctgcactacg actgcgactt cgtcagcgtg ctgcgccgct
    18901 cgccgcagcc ggcgcgcgcg gcgctggcgt tccaccgggc gctgctggcg gcgcacgccg
    18961 acgccatgac gcacgacgcc tgaccctgca gcgcttgggc agcggcgcac gaagccacgg
    19021 ccgggcgtct ggtcatggcg catcagccac cgccagcgtg aagcggaaca gcgtgccgcg
    19081 cggcaggttg ggcgcatgca ccagcgtgcc gccatgctgc tcgatcacgg tgcggcacag
    19141 cgacagaccc aggcccatgc cctcgggttt ggtggtgaag aagggggtga acaggcgttc
    19201 gaccaccggc gcatccatgc cggtgccggt gtcggcgacg ccgaactcca gcagtgcggg
    19261 gccggcgggc gcggtcggca ggcgcgcctg cagggtcagc aggcgctcgc ccggcacctc
    19321 ggccatggcc tgcaggccgt tgcgcgccag gttcagcagc acctgctcga tcatggtcgg
    19381 ctcgcaccag acggcgggca gctcgggcgc cacctgcacc cgcacgctgg cgcccagctt
    19441 gcgcgcctgc agctgcacca gcggcagcac ggcatcgacc agggcgcgcg gcgccaccgc
    19501 ctgcggcgcg gtgctgcgct ggcgcaccag ctcgcgcacg ctgtggatca cccgcccggc
    19561 acgcccggcc tgctcggcga tgcgctccag cgcggtgcgc agctcgcgtg cggtggcagc
    19621 gtcgccgccc gcaccggcgg gcaacaggtt gagcgagccg ctggcgtagc tggcaatggc
    19681 cgccagcggc tggttcagct cgtggctcat gagcgaggcc atttcgccca ccgtggccag
    19741 gcgcgcgctg gcctgcaggc gctccaggct ggcgcgcgag gattcttcgg cccggcgctg
    19801 cgccgtcatg tccaccacgg cgcccatcca gccgctgtgc tggccctggg cggtgatcag
    19861 cggggtctcg aagatcagca ccggaaagtg cgtgccgtcc ttgcgcatga actcggtttc
    19921 cacgccctcg cgcggcgccg ccgcctggcc ggacaggcgc agcgcctggc gcgcgccgta
    19981 ttcgtgggcg cgttcgggtg gccagtaggg cgcgggcgtg gactggccga tcagctcctg
    20041 cggcgtgtag ccgaccatgg cgcagaacgc cgggttgacg taggtgatgc ggccctgcag
    20101 gtcgcgcgcg cgcaggccgg tgatgagcga gtcttccatc gccttgcgaa aggccagcgc
    20161 ctcggccagc tcggcctcgg cctgctggcg ccggcggaaa tcgcgcgcca gcaggcccag
    20221 cgccgtcaac agcgccatcg acagcagggt caccagcgcc gtgagcacgt tggggaacac
    20281 gtccggcagc ggccgcccgc cctccagccg cagcagcatg ggcgcgccgg gcaggtccag
    20341 cgtctgctgc gccacgaaca ggcgcgcgcc gcgccgcgcc gcgccgtgca gggccagccg
    20401 ggtgccgtcc agctcggtga aggccacggc ctggccgcgc gtcaggctgg tcggcagcag
    20461 gcgcagcagc tcgtccagcg tgtagctggc cagcacgaag ccctgggtcc gtgcgccatc
    20521 ctgcagcggc agacacagcg tcatcacctc gcgccccagc ccgccggcgc gcagcacgaa
    20581 gctgggcggc gcgtagatcg cctcgccctg cacgctggcc tgctggcaca ggcgctgcag
    20641 ctcgggcgcc atggcctcgc gcacttcggc cggcagcggg gtgtggaacg ggctgtcgcg
    20701 cgccgcgcgc ggctccagcg ccgcgccgcg ccactccagc cgcagccact cgcggtgcgt
    20761 ctccagcagg cgggcggcgc cggcgctcca ggcctcgggg tcggcgggcg ctgcgcccag
    20821 cccgtgcagg tcctgcaggt tgcgccccag gcgctcgcgc agcacctgca ccgcttccag
    20881 gctgtcgcgc tccagcgccc gctgcacctg ttcgatctcg tgcccgcgcg ccagccacac
    20941 ggcgatcgcc agcagaccga ccaccgtcag actgagcacg ccccacagca gccagcgccg
    21001 cccggcgccg gcgcgcagcc ggcgcagcgc ggcggcgctg ccggggtcgg ggctggcggc
    21061 cgcgcgcacc ggcgccgtgg cgctcacccc agcacccgat gccgcagcgc cggcaggcgg
    21121 gcgcggcagg ccgccagccg ggccggctcc agctcggcca gcaccaccgc cgcgccttcg
    21181 tcctgcaccg ccagcacctg gccccagggg tcgaccacca tgctgtggcc ccaggtgcgg
    21241 cggccgttgt cgtgcgtgcc gccctgggcg gcggcgacca cgccgcacag gttctcgatg
    21301 gcgcgtgcgc gcagcagcac ctcccagtgc gcctgaccgg tgacgtgggt gaaggcgctg
    21361 ggcaccagca gcagctcggc gccgtcggcg gcgtgctggc ggtacagctc gggaaagcgc
    21421 aggtcgtagc agatgctcag accgacgcgc cagcggtggc cgtcgcgcga cggcaggtcg
    21481 aagcgcaccg gctgacgccc gggccgcagc accgccgcct cgtcgtagcg gcgcgtgccg
    21541 tcgtcgaact ggaacaggtg gatcttgtcg tacaccgcca cccgctcgcc ggcgggcgaa
    21601 aacaccagcg tggcgttgcg gcagtgggtg agcggcggcg ccgcgccgtc cggtgccagc
    21661 ggcagcgtgc cgcccacgag ccacaggccg agcgcgcgcg cgctgtcggc cagccagcgc
    21721 tggatcgggc cgtcgcccag cggctcggcc agggccagct tgtcggtgtc gcgctgaccc
    21781 atgaggcaga agtattccgg cagcaccgcc agctcggcgc cggcctgcgc ggcctgcgcc
    21841 agcagggcct gggcgcggtg caggttgtct tcgacccgcg agcccgagac catctggatg
    21901 gcggcgactt tcatgagcgt tcctcctcgg gggtgtcggt cgccgagcgc ggcgtcggcg
    21961 gcgtcgccgg cgcgggggtc tgggcgtcgg cggccggcgc gccaagcggc gtcacctgcg
    22021 ggtcgtccca acgcccgctg atctgaaact cgcgcgtggc cgccttgatg agcgggcgct
    22081 tgagcaccag ttgcgccacg aaggcgccaa tgccgatggc cgggttgatg gcggtggcca
    22141 ccagggccgc cgtgccggcg tcgatctcgg gcaccaccag cacgcgcaga cgctgcgtct
    22201 cgtggtcgag gtcggcctcg ccgtccatca gcaccgccgc gctggggcct ttcatctgca
    22261 ggttgttggt acgggcgatg ccggcctgca cctcgacgtc gccgcggatg aagtcgaacg
    22321 cgaagccggt cgaaaacagg tcgcggaagt ccagcgtcag ccgccgcggc agcgcctgca
    22381 ggctgagcac gcccagcagc ttggccaccc cggggtcggc cttgaggaac tggccggcgt
    22441 cgacgtccag gtgcagctgg ccggccatgc tggggtagtg cggcgaaaac ggcgtgccgc
    22501 gccagcccag accgccgcgc agctcgccgc gcccgcgcgc cagcacgtcg ggcatgtcca
    22561 gccgcgccag cagcttgccg gcgtcgcgga tgtcgagctt gaactccagc tgcgtgcggc
    22621 gccggtcctc gggcgcgcgc gggccgcgcg gatccagcgg caggccgggg ccgcgggcca
    22681 gcgccgccca gcggccatgg gcggtcagat gggcctctgg cgcttgcagg gacagccgcg
    22741 acagctccca attttgcagc gtttcaccgg cggctgcggg gcccgccacg tcatggttga
    22801 cggcctggat ttccagccgc ccaagcgcct tgccgcgcag ctcgaactgc tccaccacca
    22861 cgtccagcgc cgggatctgg gccggcggct gcgccagcag ggtgcggtcg tcgctgccgc
    22921 cggccggaat gctcaggcgc gccagccgcg ccgacagctt gccggcacgg ccgtcggcgc
    22981 cttcgcgaaa gtcgatgcgg ccggccagct cgcgcgcctg cacctcgccg cgccactggc
    23041 cgccctgctg ggtgccgctg gcaacgacgt cgttcaggct gcgcccgtcg accaccagct
    23101 cggcggtgcg cagcgtccac cgactgggca gccggccgcc ttcggccgac agcgtggcgc
    23161 cgccgcccag cacccgctgc agggcgtcgc gccagcgttc gacgtccagg tgcggcagct
    23221 cgatgccggc ctgcacgccg ctggccggca aggccagcgc cgtggcccgc gggcccagcc
    23281 cgatcaggcc gcgcagcacg cggtcgctgt cggcggcatg ctcgtactcg atcgccagcc
    23341 gctgcgccac ttccacacgc aggcgctggc gcccggcgct gccggccacc gggccgctgt
    23401 cgtagcgcag cggccaggcg gcgtcggccg gcttgtcgag cggcgccggc agatcgaagg
    23461 ccaggccgcg caggtcgctg gtcacgcgca cctcgggctc gccaccgcga aagccgatct
    23521 gcgcctcgta gctggcgctg ccggtggcct ggcgcgccag tgccggcagc ggggcccagt
    23581 cggccatctc gcgcaggccc tcggcgctgg cgctgccgct ggcgcgcagc agcaccgtgg
    23641 ggccaccctg cgccgccgcg gtgctgccgc cgctcagccg cgcctgcccg cccagcagct
    23701 gcacgcgggc gtcggcgacg gcaaagccgg tttcgctgaa ggtcaccacg ccctgcgcct
    23761 gcgtcagcag cggcgcctcg ggggcgatgc gcagctcgtt gccggccagc gcgacgcggc
    23821 cgttcaccct ggatcggtcg atggcgtcga tcggcaggtc gagcccgagc tgcaggctgg
    23881 cgtcgccgct ggcgctggca cggtccagcg cgtgctcgat gaagccggcc accggcgagc
    23941 cgcgcacgat gtcgagcgcc gaggccagca cgccgcgccc ttcggcttcg acccgcacgc
    24001 gggcgtgctg caggtcgtcg atgtcggcct gcacgcgcgt gaagcgccag ccggggtggc
    24061 cctgcacgcg ggcactggcc tggcgcaccc gcatgctgtt gcgctcgaag atcagctcgc
    24121 cggccagccc ctccagcgcc ggccagggtg gctgcccggc cggctgcagg gcgcgcggca
    24181 cgtaggccat ggtgacgccc tgcaccggcc cggcgaagcg gaattcgccc gcctccgggt
    24241 gctggtcgaa cggcagctgg cgcaggtcgc cacgcacgcg caccgccacg tcgcgcgcgt
    24301 caccgcgcac gatggcgtcg cgcacgtagt ggcgcgcctc ggccgggatc accagcggca
    24361 ggtagcggtg cacgcgcgcg ccgttggcac ggctgaagct gcccgacagg tccagcaggc
    24421 ccggaaagcg cgccgcgccc tgggcgccgg ccagcgtctg ccagcgcgcc ttgaagctgc
    24481 cggtggcgtc ggcgttgcgc agatcgagct gctcgacctc caccgcgagc tgctcgccct
    24541 gcacgcgcca gcgtccgcgc aggtcgagcc gcgccagcgg gatgcgcggc tcttcgaaca
    24601 caccgggaaa ggtcagcgcg ccgtcggcga tggccaggct ggcgcggccg ccggcgggcg
    24661 tggcctccag ctccagcgcc gcaccctcga tgccggggat gccggccacc ggctcgccgg
    24721 tggcgggtgt cgcctgcgcc ggcgcggcca gcgccaggcc gctgacccgg gtctgcacgc
    24781 gccagtcggt cggagcctcc agcgggccct gccagcgcgc ctcgatgcgc tcgaccaggc
    24841 cctgcaccgg gtgcgcctgc agccgttcgt gcaccgccgg cggcagcggc aggcgctgcg
    24901 cgatcttggc cagcgccgcc aggtcgagcc ggtcgccgct gaactcgccc ccgcccgggt
    24961 ggcggccggc ggcgtcgtgc agcgccagcc gcaggttgcc gcccggccag gtcaggccat
    25021 cggcgtcgac gaagcgcaga tcgcgcgtgc ccagcgtcat gccgtcgccg ggatcgcgcc
    25081 aggccaggcg cccggtgagt tcgctgaagg ccagcggcgc caggcgcggc cccagcgtga
    25141 ccgacaccgc gcccagcgcc agatcggccg tggcggccac cgggcggccc tgcttctgct
    25201 ccagccacag gcgcagcgcg ccgtggccgg cctgcacgtc gacgccccag tcggccgtca
    25261 gatcgacgta ctggcgcagc cgcgaaacgt cgatgcgcgg cgcgctggcg aaggcctggc
    25321 cgtcccactc gcgccagtag cccgggtggc gcgagaacac gcgctggcgg aattgaccga
    25381 tcagggtgaa gcgctcgccc caggccggct cgggcgtggc gtccagccgc aactggtggc
    25441 ggccgtagcc gttgcgagaa acgaacagca ccgcgctcag ttgcaccggc ggcgcggcgc
    25501 gctgctcgtc cacccagcgc acgcggccat cgcgcacgat gatttcgtcc tgcgagaaga
    25561 accagtcggc cgcgcgggtg tcgccggtgg cgtcacccga caggtcgatg ccaccggcca
    25621 gcagccggcc gtcacgggtg cggcgcagct ccagctcggg ggcgtcgatc accagctgct
    25681 ccagccccag ggtgcccagc gacagcaccg agaacgccgc cagcacgcgc ggcacctgca
    25741 gcgccgggcg gccatccggg tcgagcacgc gcacgtcgcg cagcgccacc gagggcacca
    25801 ggccgttcga ctgcgcttcg atggcgccga tctgcaccgg cgccgcgatg gcctgcgagg
    25861 ccagccgctc cagccgcggg cgccattcgt cgacacgcgg cacaatgacc cagtgcagga
    25921 tcgccgcggc cagcagcaca agcgccagca acacgcccac accgcggacg acccagcgca
    25981 tcaggacggc ggcaaggtgg aagaacgatt ggaccaagcg gcttgctaac gaagacagga
    26041 gcaaggccca gcgcaggcgc ggcgcgcgcc ggctggcgcc tcggagaaac gacagaaatt
    26101 atgacccctg ccgatgtgca aggttctcac ggcgacggtg aatctttgtt aagcgacttt
    26161 cgcggtctgc cgggcagcgc cgcccattcg cgcctggtgc agcggctgcg ccgccgctac
    26221 gccgacgagc tgccgctgct gcctgcgggc acccccacgc gggcgtcgat gcaagactgc
    26281 ctgacggcgc tggccgcacg cggccacgcg ctgggcccgc gcctgcgcat cctgcgccag
    26341 ctggtgatgg agcgcctggt ggtgctggac tgcgaacagg gtgcgcccct gcacgacatc
    26401 accggcgccg tcaccgagct ggccgagctg gcgctggacg ccgctttcac cgacgccttc
    26461 gcccagctcg acgagcgcca cggcgagccg ctgaccgagg ccggccagcg cccgcgcttc
    26521 tgggtcatcg gcatgggcaa gctgggcgcg cgcgagctca acgtctccag cgacatcgac
    26581 ctgatctacc tgtacgagga agacggccag acccgcggcc gcgccgacgg tcgcggccag
    26641 atctcggtgc acgaatactt tggccgcgcc gcccgcctgg cccagacgct gatcggcgac
    26701 accaccgagc acgggttcgt gttccgcgtc gatctggcgc tgcgccccaa tggcgagtcc
    26761 ggccccattg tctgctcgct cgacgcgctg gaggaataca tgctggcgca gggccgcgag
    26821 tgggaacgcc tggcctggct caagagccgt gcgctggcgc cgcgcgccga cctcgacggc
    26881 gacgccgcgc gggcgctgcg cgccgtggtg ctgcccttcg tgttccgcaa gtacctcgac
    26941 tacaacgtgt tcgagtcgct gcgcacgctg caccgccaga tccgcgagca cgccagcaag
    27001 cgcagcgccg gccggcccga gcgcgccaac gacgtcaagc tctcgcgcgg cggcatccgc
    27061 gaaatcgaat tcacggtgca gttgctgcag gtggtgcgcg gcggccagtt tcccgaactg
    27121 cgcacccgcc ccaccctggc ggcgctggag cgcgtggcgc acgccggcct gatgccgccc
    27181 gacaccgccg acgcgctggc gcgcgcctac gtgttcctgc gccgcgtcga gcaccgcatc
    27241 cagtacctgg acgaccagca gacccatgtg ctgcccaccc aggacgaaga cctggactgg
    27301 atcgcccgca ccctgggcta ccccagcgcc tgcccctttc tggccgatct gtgcgcccac
    27361 cgcgagcgcg tggcgcacga gttcgacggc ctgctgggcg gcgacgcccc ctgcaagtcc
    27421 tgcggcaaga aaagcgccga cgcccccgcg ccggcgccgc acgatctgcc cgcggtgctc
    27481 gacgcctttc ccgagcgcgt gcgcgaacgc ctggccccgt ggcaggatca cccgcgcgtg
    27541 cgcgcgctgc gcgaggaggc cctgggccgc ctgctgcgcc tgctgcagcg caccgccgcc
    27601 tggctggacg agggccgcgt cagcgacgac gccgtgctgc gcctggccga ctgggtcgag
    27661 ccgctgctgc ggcgcgaaag ctacctggcc ctgctgctgg agcgcccggc ggtgcacgaa
    27721 cgcctgctgc gcgtgctggg cacggcgcgc tggccggcgc gctacgtcat gcagcacccc
    27781 ggcgtgatcg acgagctggc cggccccggc ctgctgtcgg ggcgctttga cgcgctggcg
    27841 ttcgagcagg aactggagcg ccgccgtcag gccctggcca gcaccggcga agacgacgac
    27901 gaaagcctgc tgaacctgct gcgccgcgcc caccacgccg agacctttcg caccctggtg
    27961 cgcgacgtcg agcgccgcat caccgtcgag caggtggccg acgatctgtc ggcgctggcc
    28021 gacgcgatcc tgcgcctgac cacctgctgg tgctggccgc gcctgcgcca gcgccaccgc
    28081 gagcagccgc actttgccat catcgcctac ggcaagctgg gcgggctgga gctgggctac
    28141 ggcagcgacc tggacctggt gttcgtgttc gacgacgagc acgaggacgc gcccgacatc
    28201 tatgccgcgc tggcgcgcaa gctgatcaac tggctgaccg cgcaaacgcg cgagggcgac
    28261 ctgtacgaga tcgacaccgc gctgcgcccg aacggcaact cgggcctgct ggtgaccagc
    28321 ttcgacgcct acgccgacta ccagcaaaac cgcggcagca acaccgcctg gacctgggaa
    28381 caccaggcca tgacgcgcgc ccgcctgctg gcgccgcgcc tggcggcgga tgccgccgcg
    28441 cctggcggcg ccgcggccga gccggcgttc gacctgagcg cacgctttca agccgtgcgc
    28501 catgccgtgc tgaccgcgcc gcgcgaccac gccgcgctgc gcgccgaaat catcgccatg
    28561 cgcgagcgcc tgcaggcggc gcacccggtg cgcgccggcc tgttcgacct caagcacagc
    28621 cccggcggca tgatcgaggc cgagttcgcc acccagtacc tggtgctggc ccacacgcgc
    28681 gagcatccgg aactggagcc caacctgggc aacatcgcgc tgctgatccg cgccgagcag
    28741 gtcgggctgc tgcccgccgg cgtcggccag ggcgccgccg acgcctaccg ccacctgcgc
    28801 cacctgcagc acctggcgcg gctggacgaa cagagcaccc agatcgaccc ggccaccgtg
    28861 accgagcagc gcgccgccat ccagcggctg tggcaggcgg tgttcacgcc cgccagccgg
    28921 gcgtgacaag gcgcacggca cgcaccaccc atgggcatgg caggtcgcgg cttcgctcag
    28981 gaccaccgct cgggcgtccg cgtgccgcgg ccgcaccacc ccaccgattt ccgaatggtt
    29041 ttggcctctg gcgcccgact ggtgcgcgcc agcagctcat ttattcatag catgcagcgg
    29101 ccgccgatgg gccgggtggg cgccccatga accgctggca gcgcagcgcc tggccgctgg
    29161 cctgcctgct gctgatcgtc gccagcgcgc tggccgggct ggcggtggcc agcggccggc
    29221 tgccgcccga gctgctgagc tggcggcgtg gcgactggcc gcacgcgccc tggacgctgt
    29281 ggagcggcgc cctgctgcac cggctggcgc cgcacgccct ggccaacatg ctggcgctgg
    29341 cggcgctggc ggtgctgggc gcggcgctgg gggcgcggcc gcgcgacgcg ctggcgctgc
    29401 tggtggcctg gccgctgggc acgctgggcc tgcgctgctg gccacaggtg gccgcctact
    29461 ggggcctgtc gggcccgatc cacgccgccg ccgcagtgct ggcgctgcgc gcgctgacgg
    29521 ccgcggacac gcgctggctc ggtctgctgc tgggcggcgg gctgagcgtc aagctggcgc
    29581 tggagcacgg ctgggagtcg cccatcgcct tcgacgccgg ctggggcttc aacgtggtgt
    29641 tcgccgccca cctgagcggc gccatcgcgg gggcgctgct ggccgtggcg ctgaacgtgg
    29701 cgggctggct gcgactggcg agtgccagcg ccacggatca gcgctcaagc catggcaaat
    29761 gacgcgtgac ccttcggtca tcgaagcgca gcaaggtcat tcgtcattgg tgatcggaac
    29821 gcgcccccgc atcaggcccg catcaacgcc tcgatggcgt ccggctcgac cggcacgccg
    29881 cgcgacagca gctcgcagcc gtcggcggtg acgacggcgt tgtcctcgat gcggatgccg
    29941 atgttccaga acgcgctggg caccttctcg ctcgggcgca cgtacaggcc gggctcgatg
    30001 gtcagcacca tgccggggcg caggatgcgg ctggggcggt cctggatcag ctcgcccgac
    30061 agcgggtcgc gccgcgggct cggttggccg acctcgcccg gctccacgta gctgccgcag
    30121 tcgtgcacgt ccatgcccag ccagtggccg gtgcggtgca tgtagtgcgc aaagtaggcg
    30181 cggtgggcga tggcgtcttc cagcgtgccg tgctggccct tgtcgatcag gcccagatcc
    30241 agcatgccct gcgtcagcac ccgcaccgcc gcctcgtgcg ggtcggtgaa gcgcgcgccc
    30301 ggccgggtgg cggcgatggc agcctgctgg ctggccagca ccagttcgta cagcgcgcgc
    30361 tgcgggccgg caaagcggcc gtcggccgga aaggtgcgcg tgatgtcgct ggcgtagccg
    30421 tcgagctcgc agccggcgtc gatcagcacc agctcgccgg cgcggatggc ggcgtcgccg
    30481 gcgcggtagt gcagcacaca ggcgttggcg ccggcggcca cgatgctggg gtaggccggc
    30541 gactgcgcgc cgtgctggcg gaattcgtgc agcagctcgg cttccaggtg gtattcgcgc
    30601 acgtcctggc cggcgcgcag catggcggcg ctgcgctgca tggcgcgcac gtgggcggcg
    30661 gcgctgatct ggccggcgcg gcgcatggtg gccagctcgc cggcgtcctt gatcaggcgc
    30721 agctcgtcca gcacgcggca caggtcgtgc tgctcgcccg ggcacagcgc cccgtagcgc
    30781 acgcgcgcgc gcacctgctg cagccagccg tcgacgcgcg ccgacagccc ctcatggatg
    30841 gcgaacggaa accacaggct gcggcggttt tccagcaggc cgggcaggcg cgcgttcagc
    30901 tcgtggaccg aataggcctc atcgacgccc agcgcggccg gcgccgcctc ggggcccaaa
    30961 cggatgccgt cccagatttc gcgctccagg tccttgggtg gacaaaacag cgtgctgcgg
    31021 ccgtcgctgg tcagcaccag ccaggcgtcc ggctcggtga accccgtcag gtaatagaag
    31081 tagctgtcgt ggcggtacgg gtggtcgctg tcgcggttgc gcgggcgcgc gggcgcggtg
    31141 gggatgaggg cgatggcgtc ggggcccagc tgggcggcga cgcgggcgcg gcgggcggcg
    31201 taaggcgtgg ggtggctcat ggcgtcgatc atgacacgcc ggcgttcagc gccgccagcc
    31261 gctcgggcgt gccgacgtcg gtccaggggc cttggtacag ctcggcgccc acccgaccgg
    31321 cggcgatggc gcggcgcaac agcggcgcca gcggcgccgc cacgccctgc gggttgccgg
    31381 ccgggatgtc gcaccagggc ggctcgaaca gcgcgcgccg gtacagcgcg atggtgctga
    31441 aggtgtggcg ccgcccgccc gccggggcgt cgttctgcgc cagcccgtcg gcgccgatgg
    31501 cgaagtcgcc cgccggatgg tgcgccgggt tgggcaccag ccacaggtgg gccaggcggt
    31561 cgctggcgac gaagcgccgc accgcgtcgg cggcaaagtc gaagtcgggc gcgaacacgt
    31621 cgccggcagc gacccagaac acgtcgcggg cgcccggcgc cagcagcggc agggcgcgca
    31681 cgatgccccc cgcggtctcc agcgcgccgc cgaagtcgcg cccttcggac gagaaatgca
    31741 acccgatatg ctctgaaatc gatagctgtt ggcgcacgtc ggacaagcgc tgggccgcaa
    31801 aatgatcttc aatctgctga cccagccagg cggtattgac caccagctcc cgcacgcccg
    31861 cgcgcagcag cgcctccagc gcccactgca tcagcggctt gccgcgcagc ggcagcagcg
    31921 gtttgggaca gcggtcggtg agcggacgca tgcgctcgcc gcggccggcg gccaggatca
    31981 gggcgcgggg ggcgggatgg ggcatcgggc gactgtagtg gatcagcaga cggcctcccg
    32041 ccccaaaggt cgaacacctt cggcaagggt tcaccttaca ccctgcaggc gcaggcaccg
    32101 atgccggtca accacccggc ctgaaaggtc gcacgcagcc ggaaagggag cggcgggtca
    32161 gcgcccaaga atctgccgca gcacgctcgg cagctgagcc agctgggtgg aatcggcatt
    32221 ggccgcgcgc caggcaacca tgaggtcggg gcgcaccagc agtgcgccgc tttcctcgac
    32281 ttcgctgatg cgcaaatagt cgccgtggct gtccaccacc tcctggccaa agccaatgac
    32341 gtgcaccacc agctcgatgc ctagggcggc tgcggcctgt tcggcggtgg ccttccaggc
    32401 catgccagac aggcccgtca gcagggtgaa gcgcccttgg ccgcacaggt cgaaggtgga
    32461 gatcttgcgc tgcccgcgcg tcacccaggc gtgcggcagg tgcgcgccgg ggcggctgct
    32521 ggcttggtgg tactgcaccg ggtcgcgtgc aaagccaggg tcgggcgtgc cgtcgctgac
    32581 gatggcgctg gagcggtagc gctgattgag ctctatgccg tgggcacggc caaagccgtt
    32641 cagggtgccg tccatggcct ggcgcagctt ggcgcggcgc tcggcccctt cggcgctggg
    32701 ctccttcagc cgcgccacag cctgctccat ggcctcgggg ccgctgcccg ggggcacgcc
    32761 cagcgccatc agcagcgggc ccatgctggc accgccgttg aaggcgtgct cggtgatttg
    32821 cttggcaatc ggcgcgcgct cgtcctggta ggtgttcagc aggctggcgc cggcctggcc
    32881 ttggatgacc atggccagct tccagctcag gttgtaggca tcctgaatgc aggtgttgga
    32941 gcccagaccg ccaaacgggg tgtggcggtg cacggcgtcg ccgatgcaga acacccgtcc
    33001 ctgggtattg gtcagcgcgt attgacggtt ggtggtccag gtggagaccg acaggatctt
    33061 taccggcacc tggtcgctgc cgatggtgcg gtgcacgaag ggcaacaggt cttcgtgctt
    33121 gagcgagggc aggcccttgg ccagcgcata gcccaccacg cagacccagc ggtccatggg
    33181 gcgcaccatg cgcagcaccg cgatgccggg gccctggggc agacccatca ggttctcgcc
    33241 ggtttgcagc atccagtaca tgtcgctgcg gcggtgggcg cagaactgcg acaaatcggc
    33301 ctggatttcg atgttgatgt tgccggtctc gcccaggccc atggctccgt cgaactccag
    33361 cccgatatcg gccgccacct tggagcggcc gccgtcggcg cccaccaggt atttggcgcg
    33421 cacctggtac tcgctgccgc tgacacggtc cagcaaggtg gcggtcacgc cctcggcgtc
    33481 ctgagtgtgc gacaggtatt cggtcttgaa acggatgtcg gcaccgcgca gaccggcgtc
    33541 gttgaccagc agcggctcca ggtacagctg gggcaggtcg caatagcggc tggggctggc
    33601 ggccatgtgc tcgccctggg cgatggccga gctggcccag gccggaatac gcccccactc
    33661 ctcgcccacc agcgaccggc caaacacatg ttcgcccatg tcctcccagg gcatggcgat
    33721 tttcttggcc gcctcttcga gaccgaagtc gcgcaggaac tccatggtgc gctggttggt
    33781 gatgtgggcg cgcgggtcgg gtgaggtggc gtggtatttg ctgacggcca gcaccttcac
    33841 gcccgcgcgc gccaggcaca gggcggtggc ggcaccggcg gggccagtgc cgatgacgag
    33901 tacgtcggtc actacggtgt tcatagcagc atccccttgt gtgtcaagcg cgtttcaaga
    33961 aattgatcac cagctcattg aacgctggcg catgctccca ctgagcccag tggccacatt
    34021 gcgaaaacac atgcagctcg gccttgccca gggtgtgcaa taggcgcagg ccgtggtcga
    34081 tgggcacgaa gcgatcgtcg cgcccccagg tgatcagcgt ggggtgttga atctggtgcg
    34141 cccgggccat catgtcccag gtggcaatgg gcgacagcgc cacacaccgg aggaagttgc
    34201 tcaggtgatt gggtcgcgcc tggatgttgg cccagcgacc ctgtcgcagc tcttcggtga
    34261 tggcggcggg gttgtagaca aacacctcca gcatgctttc gaagtgggca tagctgggtt
    34321 gcttgtagag cttgatcatg tggcgtatgc cctcttgcgg gttggcctgc atgatgctgt
    34381 ggcccaggcc gccgggcccc atcagcacca gacggtccag gcgctcgggg aattccagcg
    34441 cgaagtgcag cgccgtggcc ccgcccagtg agttgcccag caggtgcgcc cgctcgatgc
    34501 ccaggccgtc catcagaccc ttgacggcac gggcattgag cagcgggcgc acctggtcgg
    34561 acaccagctc gtcgctcttg tggaagcccg ggcaatccag cagcagcacg cgaaagccat
    34621 ggtccacgaa gtggtcgatg ttcttgtaga agttgctcca gccgctggcg cctgggccac
    34681 caccgtgcag catgatgacc gcggggccgc tgccggcgtc gttgtagtgc agttggaagt
    34741 tttccaggcc gggttcgtcg atggtgatga atcggctggt gtcttcgtcg gtatggataa
    34801 gcatggtgat ctcaacagtc attcgcagtg ctgcgcaccg ctggtactca ttcagtcgtg
    34861 atggctgctg gcgcagaatg gtcgcggtgg cgcggctgtt cttctcgaca tcaagccatt
    34921 gcatggcgcc tggcctttca gtgcggctcg gggtggtggt ggccccattc gctcaggcgg
    34981 gtgtagctct tgatctccca gtcggcgtcg tcaaccacga tgccgccggc accaaactcc
    35041 agcaagaacc ctgaaggtgt gtgcacgtag aacgagaaca tgcggtcatt gggatggcga
    35101 cccaacccca tgttgatggt gtaacccgcc tgcacgcagc ggtcgtaggc cagaccgacg
    35161 tcgtcggggt tgtccacctc gaccatgatg tggtggatgc gcttgggata ggggaagggg
    35221 atcttcaccg tggccatgga gtggtgccgc ccggtgcgag catggaaaaa cgccacgtcc
    35281 agcagcacgc ccggggccag ctcaccacga atgaagtcac tgatcttgat gcccagcacc
    35341 tgcttgcaaa aggcgatggt ttcctgcgca tcgttggccg ctgccacgaa atgccccatg
    35401 cccaggcggc cagtggaaaa accgcccacc aacttgctgg aatgaaaggg gttgaccatg
    35461 ctggcgcgtt gcgcgccgac gtagaactcg tgccggatgc cgttggggtc ggtgcagaag
    35521 tacaggtctt ccacgcgccg ctctttggcc agcgccgcgt cgccctgttg cacggccaca
    35581 ccctgctgcc gcagctgggc ggcaaaggca ttcaaatctt cctgggtatc gagctcccag
    35641 ccggcggcca gcatgtcatc gtcgtcatga cgctccagca cgatgcgctg ttcgtagcca
    35701 tccatgcgca gcgccagcag tttgccgggc tcgctgcgcc cgggttgcag gcctatggtg
    35761 tccacggcga attgcttcca ggcttccagg tccttcacac ccagcaccag ataccccagg
    35821 ttcgaaatgc ttgccatcgt ctgtctccta taaacgaccc cgcccacgcg gtcagggata
    35881 tcagagctct gcgagaaccg tgccggccag gaaggattga cccaaatcca ggggaaaacc
    35941 ctctgtttgc cgctgcgctt ttgtgggttt tcctcgctcg ccccgggtta tccctcgcat
    36001 atctgaggcg acccaggttc caaaattgat ggatttcata atttcatgaa gattcttgtg
    36061 aatcattgcg cagcagccgc ggcccgccat gcccgatagc acaccccgcg cccccgtcct
    36121 gcaaatgccg caagtggtgg tggacatgaa gctgccgcaa atccgcgagc tggcgacgca
    36181 gttgcgattt gcgcccgagg aagggcgcat ctggatggat gagcggcgta tggtcctggt
    36241 gcacagcgac acctttgccg acctgcgcga ggagctgctg gccagcctgg gctatgaaat
    36301 gacgcgcggg ctgctcacgc gcatctactt cgctgccggc cagcgcgatg ccgccagcgt
    36361 gctgaagagg ctgggtccgg gcgcgagtct gaaagaggtg ctcaacaccg gtggacgcat
    36421 gcacgcccta caaggctatc tgctgcctga gcatgtcggc cgtgggttgg ccgccggcga
    36481 cttgcgcagc gacgactatt acggcgaggc cttgtggaag gactcgctcg aagacgaggc
    36541 acatgttgcc cgccatggcg tgggtcagca cgccgtctgc tggaatgccg tgggctactg
    36601 ctcaggctac ctgagctact gcgcgcaacg caccattgtg gtgcgcgagg tcgagtgccg
    36661 cgccagcggt ggcaaccact gcaagatcgt cgctcagcct gccgcccaat gggacaacgt
    36721 ggaggaggat ctgcgttttc tgctgccgca gcccgtgggc cggcgcgcca gaatcgtcgc
    36781 ccccgtcgcc gatggcgggc ccaccactgc ccttgcccag agcgccgtca ccgacagcag
    36841 agacgatgac cgcgcggcgc cgcggtcgct ggcagaaacc gcctttgttg gcgcctcgac
    36901 cgccttttcc gtgctgcggc acaagatcga ccgcgtcgcc cccacccagg ccacggtgct
    36961 gctgctgggc gaaagcgggg tgggaaaaag cctgatcgca cgccaggtgc accgacgcag
    37021 ccagcgcgcc caggcgcaac tgattgaagt caactgcgcc gccatcccag aggcgctggt
    37081 ggagtcggag ctgtttgggg tcgagcgcgg ggccttctcg ggcgccagtc aggcccgcgc
    37141 cgggcgcttt gaggccgcgc atggaggcac gctgtttctg gacgaggtcg caacgctcag
    37201 cccgaccacc cagggcaagt tgctgcgcgt gctgcaaagc ggtgcgctgg agcgtctggg
    37261 cagcaatcag acccgcgtga ccgacgtacg cgtgattgcc gccaccaacg aagaccttaa
    37321 gcaggccgtc cgacagggac gctttcgaga agacctttac tttcgcctca atgtctttcc
    37381 ggtggtggtg ccgccactgc gcgagcggcg cgacgacatt ccactgctgg tgcaggcgct
    37441 gctgcagcgc tttgccccgc tacacgggcg tggccacgtt gggatcacgt cgcgggcact
    37501 gcaagcactg atccaccacg actggccggg caatatccga gagctggaaa acgtcatcga
    37561 acgcgccgtc atcatgctgc atgaaggcga aatgctggac ttgcagcacc tgtcctacgc
    37621 cgatgacacc ctgaacaccc cgagctactt cggcctggac gacacggggc agttaatgat
    37681 caacaccgat tacgacttcg gcgcaagcca gtcaccggca tcgacgagtg cggccagtcc
    37741 tgcggcactg gacatgaacc ggttggccga tgacttgctg cgccagggcc ccggtcagct
    37801 ggccgccatg gagcgcgccc tggtgcgcag cgccgtgcgg caggcggggg gcaacatcac
    37861 tcgtgccgcc gcactgctgg gtctgtcgcg ggcacaaacc gaataccggt acaggaagca
    37921 gcaaagcgcg ccgggatgac gtcgcgtaga cgcgggcaaa ggaggcgagg cctccgaccc
    37981 tggccgcccg cgtcttaggc tggggtcaga gctggaccca gacgcgccca gcctcaatct
    38041 tgacaggata gctgcggatc gggtcgacac atggggccag agtggcttgg ccggtgcgca
    38101 cgtcgaaccg cccctggtgg aacgggcact cgatctcgtg accttcgagg aaaccctcgc
    38161 acagccgcgc atggccatgc gagcagatgt tgtgagtggc gaagacttcg cccgcctcgg
    38221 tcttgtagag cgcgatgtcg tgccctatcg gcgtgacggc gatggccgcg ccctcgaaga
    38281 tgtcgtcctc ggcggcgacg tcgatccatt ggctcatgtt cggatttcct gctcagatcg
    38341 ggtagatgat ggagttcgga atcatctcgc tgtcgtacac cacaaggcgc tcggccagct
    38401 tcaacccgag cggcgtgcgc accacgcggt ccaggtagcg gcccacgttg aacacgctgg
    38461 acagtccgtc gagcttggtg cggaacaccg cgtagtggct ttcgcagcgg atgccgtcgc
    38521 tgtctgcggc gtgcaccagc ggcgcgccga ccacgtggcg ctggtagtac gggtcatgga
    38581 acagcgtctc cttgatgccg tagacgcgat cgcgcatggc cgcctggcag tcaaaggcca
    38641 gggtgcacag cggaaagccg cgctcatggt tttcgcgtgg ttgcagcttg tacacaccgt
    38701 cctcggtgaa aaaccggggc cagcggtccc agtcgccacc atcgacgacg gcggcatagt
    38761 cggcctgcaa ctgcaccagc gcctgccagg tgtcgaaatc gagcctcatg cctgcgcccc
    38821 ctcttgcggc gcgaccgcat cggacgcctg gtcagggcgc tccatgacct tgcgccagta
    38881 gtcgtacatg ccacggatca gcgtctcggt ggccatgtag tcggtctcct cgacgccgcg
    38941 cccgcccatc tcgaccagcg cgtggtgccc cggcttctgg gtgaagccgc attgcgacag
    39001 ctcgatcgct tcgccgtcgt cggccgacac gaaaccggcc ggaccgaaca ggttggcctg
    39061 gcgcagacgg cgcgccgtca tctcggccgt atcgtcggca aagccgaagt gcgtccagac
    39121 gaagtcgaaa tggccatggc cgtccggctg gatgtgccgc gtcgacacgc tgttgacctg
    39181 ctgctggaag atcacgctgg ggaacacggt ggtcatgacc accgtcggct caccccacca
    39241 gtcctcgtgc acgatatcca ggaagcgctt gtcctccagt tccatggcgg ccttgaagct
    39301 cgacacttgc gacacatcgg actgcttgcc ggcattgccg cgcgtcgaga tcatggccgc
    39361 gtggcgatgg tgcgcatcca tcctgagcgc tgccttgttg tcggcgcgcc aaagaccgaa
    39421 agtgacgaac caggtatgca gcaaacccgg gtgatagggg tccttgatgt tttcctgcat
    39481 cagcttccag ttgcctggga tgcgctggcg gttgtagcca aggatcttca attgacgtcc
    39541 gttgaacaat cgatcgaagt agggcaggat cgtagggccc aggtaatcct ccagcggttc
    39601 cacatcatgg tcgaagctgg cgaaaacgac accaccccgg gtggcgactt tgagccgtgt
    39661 caaaccatgc gccttggtgt cgaaatccgc gggcatgccg cccaacaccc gcccatcctg
    39721 gcgcacgcca cgacgcagcg gcaccccctg cagttcgcca ctgagcgtat agctccactg
    39781 gtggtagggg cagacgaact ccttcatgcc gctgccatgc cgcttgcgac agaactgcat
    39841 gccgcggtgt gcgcacacgt tctcgaaaca atgcagctgg ccatctgcgg cacgactgag
    39901 gatcactgag cgctcaccca ccacggtgcg cttgaaatcg ccaggacggg gcacttcagc
    39961 ctccagaccg acgtagcacc agtggccacg gtagaagaag cgctgcaact ccttgcggtg
    40021 gatttgctcg ttggtgtaga tggcgaatgg cacgcggctg gtgtcgccag cgggccaatc
    40081 aagcgatgtg gacgggatca agccaagtct ccgtaagtga tgcaggcacg cgccttgcct
    40141 gacatgatgc cgagcatgag tccctgcctc aagctcgcat ctggaatgtg tgatatttcg
    40201 tttgagaata atgtcgagat ggatttgtcc tccattgacc tcaacctgct gcgtatcctg
    40261 catcgcctgc tacaggagcg ccacgtttca cgcgccgcgc aagacctagg tctgtcgcag
    40321 ccggcggtga gcaatgcgct gcgcagactg cgcgtgcagc tgggggacga tctgctggtg
    40381 cgcagcgcgg ctggcatgca gcccacgccc ctggcccaac gcttggctgg ccccatcgca
    40441 caagcgctgg aactgctcga cgatgcgctg cgtgcgcagg cgccattcga cccagccgac
    40501 agcgtgcgcc agttcacgct ggccatgtcc gatgtgggag agctctattt cttgcccgtg
    40561 ctcatgcgag ctctggccca gctggcgccc ggagtcacct tgcgcaccac agcgctggac
    40621 agccccgggc tggggccatc gctgtccacc ggcgagatag acctggcgct gggttcgctg
    40681 cctcgcctgc aaggaggctt ctaccagcaa tcgctgtttc gccaaggcta tctttgcctg
    40741 atgcgcaatg ggcacccgat ggctgagggc ccgctgacgc gcgctgcgtt cctggcctgc
    40801 gagcacgtgc gcgtggaggc tccgggcacc ggtcacggcc gcgttgacag cgagttcgag
    40861 cgccgaggca tcaggcggcg gattcggctg tcggtgccga attacgtggc gttgggccac
    40921 gtgctggcca gcaccgacct gattgccaca gtgccggagc gcttcgccga acgggtctgc
    40981 gccgcgctgg cgctgcgcac gcatcccctg cccgtgcgca ttgcctccgg tgtcattcgc
    41041 cagttctggc accagcgttc acatcgcgac agcggccacg aatggcttcg gcggcaggta
    41101 tcgagcctgt tcgccgacag cgccacccga gcctgatgac tctcgcccga cctgtcggcg
    41161 tcgcgacggc gctggcagat cagattcatc cggcggcgaa catgttcacc accacccgca
    41221 cccggctgcc gcgtgccccg gctgccgcgc ttgctcgggt tacgatggca cacgcgcctg
    41281 caggcgaggc ggtgcgacta catcgcctgc gccgccaccc caccacccca tgaccgcgcc
    41341 ctgcctgctg gccatcgact gcggcacgca gagcgtgcgc gcgctgctgt tcgacctgca
    41401 gggccagctg ctggccaggg cgcaggtggc catcgacagc taccgcgcgc ccgagccagg
    41461 ctggatggaa ggcgcgcccg aggccttctg gcagcacctg tgcgccgcct gccatcggct
    41521 gtgggccacc ggcacggtgc cgcgcgaggc gatcgccggc gtggtgctga ccgcccagcg
    41581 cgcggtgccg gtgacgctgg acgccgacgg ccagccgacg cgcccgtcga tgatctggct
    41641 cgaccagcgc cgcgccgccc gggcgccgcg cctgccctgg tggtgggagg cggcgctgac
    41701 ggcggttggc atgcgcgcgg cggtgcgcca cttccagcac gaggccgagg cctgctggat
    41761 cgcccagcac cagcccgagc tgtgggcgcg caccaggcac tttctgctgc tgtcgggcta
    41821 cctgaactgg cgcctgtgcg ggcggctggc cgactcggtg gcggcgcagg tgggctatgt
    41881 gccgttcgac taccggcgcg ggcgctgggc cgcgccctgg gactggaaat ggcacgcgct
    41941 gccggtgcgc cgcgccatgc tgcccgagct gctgccgccc ggcgcgcggc tgggcaccgt
    42001 gagcgccgag gcggcgcagg ccagcggcat tccggccggc ctgccgctga tcgccggcgc
    42061 cgccgacaag gcctgcgagg tgctgggcgc gggctgcctg tcgcccgagg tggcctgcct
    42121 gtcctacggc acggcggcca cggtcaacat caccaccgca cgctacctgg aagccacgcg
    42181 cttcatcccg ccctacccgg ccgccgtgcc gggccacttc aaccccgagg tgcagatcac
    42241 gcgcggcttc tggatggtgc gctggttccg ggagcagttc ggtcagcacg aagagcgcct
    42301 ggcgcgcgag cgcggggtgg cgcccgagac cctgttcgac gaactggtgg gcaccgtgcc
    42361 gccgggcgcc cagggcctga cgctgcagcc gttctggaac ccgggcatca agcaacccgg
    42421 ccccgaagcc aagggcgcca tcatcggctt tggcgacgtg cacacgcgcg cccacctgta
    42481 ccgcgccatc ctggaaggac tggcctacgc gctgcgcgag gcggccgagc gcgtcgagcg
    42541 gcgcagcggc atcgccatcg aacgggtgcg cgtggccggc ggcggctcgc agagcgacgc
    42601 cgcgctgcag atcaccgcca acgtgttcaa ccggccggcc gaacgcccgc acctgtacga
    42661 gaccagcggc ctgggcgcgg ccatcatcgc cagcgtgggg ctggggctgc agcccgactt
    42721 cgccagcgcg gtgcgcgcca tgacgcgcgt gggccaggtg tttgcgcccc agcccgagca
    42781 tgcgcacact tacgagcgct tgtaccggcg cgtgtacctg cgcatgtacc gccagctggc
    42841 gccgctgtac cgcgacatcc gcgacatcac gggttaccct tcacgcgact gaaccgccct
    42901 gcccatgaaa cgtctgctga tcaccgtcgc gctgagcttc ggcgcccatc tttccgccca
    42961 ggcctggagc aaccacgcgc tggccagcta ccgcgccttc gagcccatgc ccgaggtggc
    43021 ccaggccccg gccgtgctgg ccgagccgct ggagcagttc ctggccgcgc aggcccagcc
    43081 gatcgccgac ctgctgcagg cgcaggacgc ctgggcgcag gcgcatctgc cgcactaccc
    43141 gccgctgccg gcagccctgc gctttgaccc ggcggtggcc agccagggtc cgcaggcgct
    43201 gcggcgggcg ttcctgacgg cgctgcgcgt gtcgcccgac agccggctgg cgttgtacgt
    43261 gcagcccgac ccctggggcc ccgagccgca ggccgagcgc ctgccgcacg acgccgtcag
    43321 cgcgctgccg ctgcgcgaca aggacggcga aaagcaccgc ttcgtgcgcc tgcacgccgg
    43381 ccaggccgtg gccccgatcg ccgtgctggc gtcggccagc gatgaacccg actacggcat
    43441 ggacctgaac ctgtggcagg acagcccctc cagctggggc ccgggctacg gcttcggcca
    43501 catcccgttc ggcaacccca gcctggactt cgccacccag gcgccgtttc acatgggcta
    43561 ctaccacgag tcgggcctga tctacgcggc ggccgggttt ctgaaacgca cctacccgct
    43621 gctgcgcgcc caccagtacc agggactggc gcagctggcc tttcgccgcg gccacccgta
    43681 ctggggctgg cgctttgccg ggctggcgct gcactacctg caggacctga cccagcccta
    43741 ccacgccagc ctggcgccgg gctactcgag cgcgcgcctg atcggcatcc agctgctggc
    43801 cctgctggga cggcccggcg ccaagaacga catggtggtg cgcctgtcca accgccacct
    43861 ggtgctggag cgttacgaaa gccagatgat ccaggccagc gcccaggccc gcaaggccgg
    43921 cccgctggag caggcgctgc gcgacaccgc ggccgacgcc agctacggcc cgtggagcga
    43981 taccacgctg cgcgacgaag tggcgcgcca ggcgcacgcc gccggcgaaa cggtgacggc
    44041 gcagctgctg gccaccgtgc ccgccggcta cgtcgatgac ccgacgttcg acttcggcgt
    44101 gcacgccggc ggcatcgacc tgatggccga ggtggcgcag cgcggcgcgc ccgccaagca
    44161 ggcgctggaa cagaccctgg ccacgctgat gcgccatttc ggcgtccaca gccggcggct
    44221 ggtgcgcgcc atcctgcaag gcgccgcacc ctgacacaag caccgatcca ccctgaccca
    44281 tgttcgacgt cacccccctg tccgccaccg accgatccga gcaatacgcc gagctggcgc
    44341 gccaggtcga cgggctgctg cacggcgaaa cgcagctgat cgccaacgcc gccaactttg
    44401 ccgcactggt gtttcacgcc ctgcccgagc tgaactggtg cggcttctac tttgccgaag
    44461 gccaggagct ggtggtcggg ccgtttcagg gcaaaccggc ctgcgtgcgc atcgccttcg
    44521 gtcgcggggt gtgcggcacg gcggcggcca cgcgccagac ccaggtggtg ccggacgtgc
    44581 atgcctttgc cggccacatc gcctgcgaca gcgcctcgcg ctcggaaatc gtggtgccgc
    44641 tgctgcgccg tgacggcagc ctgctgggcg tgtgggacgt ggacagcccg cggccggcgc
    44701 gcttcgacga ggtcgaccgc gtcggcatgc aggcgctgtg cgcgcgcttt ctgcgcgcct
    44761 gcgcgctctg acgctccgcc agccccagcg cggcggctca gccggccagc tcggtcggca
    44821 tctcgggccc gatcttgaac accccgctgg cgcgcgcgca gggttcgcca tcggcggtca
    44881 ccaggccctg cgcgaacacc agcgtgcggg tggcgcgcag cagctcggcc gtgccctcga
    44941 tccagcagcc cagcggggcc ggcgccaggt agtcgatctg caggctgatg gtgggcagaa
    45001 aacgctggcc gagcccggcg cccttgcggt gcaccgacag cggcagcagc atgtcgcaga
    45061 agctggccat catgccgccg tgcaggttgc ccatgggatt gcagtggcgc ggctccaccc
    45121 gcaggccgaa gcgcacgtcg tcgccctggt gccgcagata gagcgggccg ttgaaggcca
    45181 tgaagccgcc gcccgcctgc agcggatgaa agccggccgg gatgtccggt gaagtgcaag
    45241 ggtccatggg gcgcaggata agcccgcgcc acgtggcggc ggtgcggcgc gcgtgccgca
    45301 ggcgacagcc gcccggcttg cggggccatg cgcggcgtcg ccgcgccggc cctggggggc
    45361 ccggtaaaat cgccggtttc ccccctcctg caccgccttc cggagccgct ccatggcaaa
    45421 tccttcccgc atggccaacg cgatccgcgc cctggccatg gacgccgtac aacaagccaa
    45481 gtcgggccac ccgggcgccc ccatgggcat ggccgacatg gccgtggcgt tgtggacgcg
    45541 gcacctcagg cacaacccgg ccaacccgca gtgggccgac cgcgaccgct tcgtgctgtc
    45601 caacggccat gcgtcgatgc tgctgtacgc cctgctgcat ctgtcgggct acgagctgcc
    45661 gctgcaggaa ctgaaggact tccgccagtt gcacagcaag accgccggcc accccgaggt
    45721 cggtctgacc cccggcgtgg aaaccaccac cggcccgctg ggccagggcg tgaccaacgc
    45781 ggtcggcatg gcgctggccg agaagctgct ggcggccgag ttcaaccgcc ccggccacgc
    45841 catcgtcgat caccacacct acgtgttcct gggcgacggc tgcctgatgg agggcgtcag
    45901 ccacgaggcc tgcgcgctgg ccggggcctg gaaactgaac aagctgattg cgctgtacga
    45961 cgacaacggc atcagcatcg acggtcaggt cgcaccctgg ttcgtcgatg acgtgccggc
    46021 gcgctttgcc gcctatcggt ggaatgtgat cgggccgatc gacggccacg acgtcgacgc
    46081 cgtcgatcgc gccatcgcgc aggcgcgccg cagcagcgac cggccgacgc tgatcgtctg
    46141 caagacccac atcggcaagg gcagcccgaa ccgccaggac agcgccaagg cgcatggcga
    46201 gccgctgggg accgaggaaa tcgccctcac ccgcgccgcg ctgggctggg acgccgcacc
    46261 gttcgagatc ccggccgacg tcggcgccgc ctgggatgcg cgcgccgccg gccaagcccg
    46321 cgaagccgag tggacgcagc gcctggcggc ctaccaggcc gcgcacccgg agctggcggc
    46381 cgagctgctg cgccgcctgc gcggcgagct gccgccgaac ttccatcagg tggcggtgga
    46441 cgccgccgtg gcggcgcacc agaaagccga gaccgtggcc agccgcaagg ccagccagat
    46501 cgcgctggag gccttcaccg ccgcgctgcc cgagctgctg ggtggcagcg ccgacctgac
    46561 cggctccaac ctcaccaaca ccagccacac gccggcgctg cgcgtcgacg cggccggcga
    46621 tgtggtgcac gacgcccagg gccgcatcgg ccgccacatc aactacgggg tgcgcgagtt
    46681 cggcatggcc gccatcatga acggggtggc gctgcatggc ggcttcatcc cctacggcgg
    46741 caccttcctg accttcagcg actacagccg caacgccatc cgcatggcgg cgctgatgaa
    46801 gcagcgcgtg atccacgtct tcacgcacga ctccatcggc ctgggcgagg acggccccac
    46861 acaccaggcg gtcgagcacg ccgccagcct gcgcctgatt ccggggctgg acgtgtggcg
    46921 tccggccgat accgccgaga ccgtggtcgc ctgggcggtg gcgctgcagc agcgcaccct
    46981 gccgacggcg ctgctgctgt cgcgccagaa cctgccctac tgccccaggc gcgacctggg
    47041 cgacatctcg cgcggcgcct atgtgctgag cgagccgtcc gacctgggcc tcaagcgcaa
    47101 ggcgcaggcc gtgctgatcg ccaccggctc cgaggtgcag ctggcgctga aggcgcagga
    47161 gctgctggcc cgcgagcacc gcatcgcggt gcgcgtggtg tcgatgccct cgaccaccac
    47221 cttcgaccgc caggagctca agtaccaaaa ggccgtgctg cccgccggcc tgccgcgcat
    47281 cgccgtcgag atgggtgcca ccgacggctg gtggaagtac ggctgcgccg cggtggtggg
    47341 ccttgaccgc ttcggcgagt cggcgccggc gccggtgctg ttcgagcact tcggcttcac
    47401 gcccgagaac gtggccgaca ccgtgcgtgc ggcgctgcgc cggcgctgag catgccggag
    47461 cctccctacg cagacaacgc agacaacgca gatgatgttt cgtttccctt ctgcgccttt
    47521 tgcgcaatct ctgcgcgctc tgcctacggg tgtttgatgt ttgataacaa atggcctcgc
    47581 agcgcttgat ggataagcgc aaccagctat cgatttacta gcaaccaagg aaactgtcat
    47641 gaccatcaag atcggtatca acggcttcgg gcgcatcggg cgcatggtgt ttcgcgcggc
    47701 cgtcgccaac tacaaggaca tcgaggtcgt cggcatcaac gacctgctgg agcccgacta
    47761 cctggcctac atgctcaagt acgacagcgt gcacggcgtg tttgcgggcg acatcgcggt
    47821 cgagggcaac accctggtgg tgaacggcaa gaagatccgc ctgacggccg agcgcgaccc
    47881 cgccaacctg aagtggggcg aggtcggcgc cgacgtggtg atcgaagcca ccggcctgtt
    47941 cctgaccaag gaagcggcgc agaagcacct ggacgccggc gcccgcaagg tgatgatgag
    48001 cgcgcccagc aaagacgaca cgccgatgtt cgtgttcggc gtcaaccacg acacctacgc
    48061 cggccaggcc atcatctcca acgccagctg caccaccaac tgcctggccc cggtggccaa
    48121 ggtgctcaac gacaagtggg gcatcaagcg cggcctgatg accaccgtgc acgccgccac
    48181 cgccacccag aaaaccgtgg acggccccag caacaaggac tggcgcggcg gccgcggcat
    48241 cctggaaaac atcatcccgt ccagcaccgg cgccgccaag gcggtgggcg tggtgctgcc
    48301 cgcgctcaag ggcaagctga ccggcatgag cttccgcgtg ccgacctccg acgtctcggt
    48361 ggtcgatctg acggtggagc tggacaagcc cgccagctac gccgagatct gcgccgagat
    48421 gaaggcccag agcgaaggca agctcaaggg catcctgggc tacaccgaag acaaggtggt
    48481 ggccaccgac ttccgccacg acgcgcgctc ctcgatcttc gacgccgacg ccggcatcgc
    48541 gctggatgcc accttcgtca agctggtgag ctggtacgac aacgagtggg gctattccaa
    48601 ccgctgcctg gacatggcac gggtgatgag ccagaagtaa gccgccggcg gctgcaacga
    48661 aaaacgcgcc ctggggcgcg tttttcatgt ccgtcgcgcg ccctcgcacc ggctgcagca
    48721 cgtgaatcac gcggctggcc acccagtcgc ggcaaggttg ggagggatcg ccgcgtgggc
    48781 gctccggtgt ggtgcggcgc cgcgcgcgtt acagagatcc ggcggctgcg gtcggcgctc
    48841 atgctacaac acgccatgcg catcctcacc gcccacctgc tccccgccct ggccctgggc
    48901 accgccctgc tgaccagcct gcccgcacgt gccaccgacg ccaccgtggc gcgcggcctg
    48961 atcgtcacgc tgaaggcacc gtccgacggc gggcgcgaat cgccccaggc cacgcgcgag
    49021 cgcctgcggg ccgtggccgc cgacgccggc ctgggcggcg cgcaggcgcc gatgccggtg
    49081 ggacctgggg cgcacctgct gccgtttgcc gccccgctga ccggcaccgc cgtgcaggac
    49141 gccgagcgcc gcgtgcgcct gaacccgcag gtggccagcg tcgagccgga cgtgcgcctg
    49201 aagcgcctgg ccacgcccaa cgacccgtac tacaccgacg gcatccagtg gcatctgcgc
    49261 acgccggccc aaggcggcgc cgccgccctc aacctgccgc cggcctggga gcgcacgcgc
    49321 ggcagcgcgg agcaagtggt ggcggtggtg gattcgggcg cgctgtacgg ccacccggac
    49381 ctggccggca agctgctgcc cggctacgac ctgatcagcg acctggacac cagcaacgac
    49441 ggcgatgggc gcgaccccga cgcctccgac ccgggcgact ggatcacgtc gtccgaagcc
    49501 ggcagcgggc gctatccgct gtgcccggcc gaggacagct cctggcacgg cagcttcatc
    49561 gccggcgaga tcgccgccgc cacccacaac ggtgtgggcg tggccggcgt gggctgggac
    49621 acgcgggtgc agccggtgcg cgtgtcgggc aagtgcggcg cctggctgtc ggacgtggtg
    49681 gagggcattc gctgggccgc cggcctgccg gtcagcggcg tgcccaccaa cccgacgccg
    49741 gcacgggtga tcaacgtcag ctttggcggc agctccagct gcaactcggc ctaccagacg
    49801 gccatcgacg acgccggcgc cgccggcagc ctggtggtgg tggcggccgg caacgaaaac
    49861 ggcgccctga cgcggccggc cgactgtgcg ggcgtgctgg cggtgggcgc ggcacgtcag
    49921 gacgggctga agacctacta ctccagctac ggccccaacg tcggcatcat ggcgccgggc
    49981 ggcccgggcg ccgccgacac cggcccgcgg ctgtattcca cctccaacgc cggcctgcgc
    50041 gggccgggcg agcacatcta cgacagcaag gacggcacca gttttgcgtc gccgctggcg
    50101 gccggcgtgg cggcgctgat gctgtcggtc aaccccgcgc tgacgccggc gcagctgatc
    50161 tcgcgcatcc agtcgggcgc gcggccgttc cccagcagcg cggcctaccc cacctgccgc
    50221 gtcggcatgc cggccagcag cgcctgcaac tgcacgcgcg agacctgcgg tcccggcctg
    50281 ctggacgccg accgcgcgct gcaactggcc accagcccgt cggcggtgat cgcgccgatc
    50341 ggcagcgtgc cggccggcag caccgtcacg ctggacggcc ggggcagcgc ggcggtggcc
    50401 ggcgccagca tcgccagcta ccagtggagc caggtctcgg gtagcccggt cggcatccgc
    50461 gacgccggcg cgccggtggc cagcgtcagc ctgcccgacg cgcgcggcga ctgggtgttc
    50521 cgcctgctgg tgaccgattc cgagggccgc gccgccgaca actacgtcac cgtcagcacc
    50581 ttcggcggcg acgtggacga aggcggtggc accaccggtc cggcctgggg cgccggcctg
    50641 tgggcgctgg cgctgggggc gctgtggcac cgccgcaccc gccgccgcac ggccgccacc
    50701 agctgatgcg ggcgccggcc atactattgt tttgatagct gttggcgctt gccagacaag
    50761 cgccggagcc tgatctgacc aagaacggtc agggacttcc cgcgcacccg cgttcccaat
    50821 ggcccatgcc ggatgagctc gctgcgcttc gatgaccgat gggttcggcg cagccggtgt
    50881 catgggacgt ttttagcgtg aaacggtatc aggcgcgctc ggacggtgcg ccggtctcgc
    50941 gcgccacgtc ggccaggaag tcgccctcgc ccagcgcgct gtcggtgcca cggcgcagcc
    51001 acgggccgta gacgcggtcg aagtcggcca cgatgcgatc gagcggcagg tcgtcgaagc
    51061 tgccgcgctg ccggacgtat tccatgtgcc ggttgccgct ggcgtcgaac gggtggtaga
    51121 tcgagtcgtg cacgccgtcg aagtcgagcg gcagcaggtt gaatttctga cacaggctga
    51181 ggttgaacgc cggcgtggcc ttgcgccagg cgccgtccag ccacagctcg gtgtagccgt
    51241 gccagatgaa caggtcggtc tgcatgctct ggcgcatgcg ctcggtgctc aggtggttgc
    51301 gcacgtcggc aaagcccagc cgcgccggga tgccggcggc gcgcgccgcc gccgccagca
    51361 gcaccgcctt gggcacgcac cagccgtagc cgtgggcgat cacggtgctg gcgcgcatgc
    51421 cggcgggcga caggtcgatc cggtaggggt cgtagcggaa ggcgtcgcgc acggcgtgcg
    51481 tcagcgccac ggcgcgctgg cgctcgctgg cgccttgcgc gtgctggcgc gccagggcct
    51541 gcaccgccgg gtggtcgcag tcgatggcgg gcgtgggcgc caggtgggcg ggggtggggg
    51601 tgtcgctcat ggttcccatc ttagggcgac actgaaccag tccttctcgc gccgcgcatc
    51661 cctggtgtga aaggtctgcg gtgttgcaac tgctcgtcat caccatggct gtgggtgggc
    51721 tgatggagct tgccatccat cctcaatcat ggcgaagggc gcgcgccatc gcagggactt
    51781 gttcagcgtc gccttggtgc gcgtggtttt ctctcgcacc actcggattg gcgtcgtcag
    51841 tgaccattgg ccagtccctc tgccagcgcc tcaaaaacca cccgaatgcg ccgggatgtg
    51901 cgcagctctc gatgcgtcac cagccagatc gggaacctta cgggcgccac atcgtccagc
    51961 acccggacca tgcccggggt ctctcgagca atctcgtcca tcatcgcacc gatgcccaag
    52021 ccctggcgca ccagagccca atgggtcaca ctgtgatccg cgtagcagct gaagctgtct
    52081 tcgctcagat tcagcccatg atcgcgcagg tatgcgagat accggccatt gcgatccgac
    52141 cccacaaacg gcagcccggc agcgtccgtt gcagtgcggg gatggccgta cgtctgaacc
    52201 caagattcag acgcataaaa aagcgcctgg gcttcgcgca ccaggcgggc gatcaactca
    52261 ggctgttcag gcttgacgtg gcggatggcg atgtcggcct cgcggcgcag caggtcgctc
    52321 acggcgttgg atgaaatcac ctcgatcgca atgcccggct cctgggctcg caagcgccgc
    52381 acgatgacag gaaggagatg ggccgccacg gcatcgctgg ccgacaccgt caccacgccg
    52441 cccaccgctt gcgacctgcc gttggcggcc aagcccaggg cctctgccgc gttgcccatg
    52501 gcacgggcgt gctccagcag atccagccct gtgggtgttg gcaccatgga cttgcccacg
    52561 cgctcgaaca gggtcacccc catttgccgc tcgatggctg ccacctgccg actcagcgtg
    52621 ggctgggtca agcccagctt gcgagcggca gcagacaggg agccgctctg cgcggtgtgc
    52681 agaaaggcct tcagccaatt ccagtcaagg tcattcatac ataaatgcat gtatccactg
    52741 caattcaaag ccattttcta cgcttcattg catggatagc atctgggggt tgctcgttgc
    52801 aaatgcctgc gatcaccact tcggcatgtc ctacggtctg caccctatgc tcttgcctcc
    52861 caataccacg gtccgtgaca cctcaccttc ggccgcatcc cgttcggctt ctgcgtccaa
    52921 ggcacggttt tgggaccgca tcgcccccaa gtacgcagcg gaccccattg ccgacatggc
    52981 ggggtatgaa accacattgc gccgcgcgca ggagctgctt tccccccaac aagatgtgct
    53041 ggaagttggt tgcggaacgg gccatacggc gctgcgcctg gctcccttca cccggcgttt
    53101 gatggccacg gatgtgtcgg cggagatgat tgccattgcc tgtctgcggc aagctgcaca
    53161 accctcgccg caactcacgt ttgccctggc cgatgccgag gccaccgact ttgggcgagc
    53221 cacctatgac gcggtgctgg ccttcaatgt gctgcacctg acgagcaatc tgggtgatgc
    53281 actgaagccg ttggtgcatg cattgcggcc aggtggactg ttgatttcca aaacgccctg
    53341 cattgccgaa atgcaccctt ggattcctta cttggctgta ccgttgatgc gggccattgg
    53401 aaaagcgccg cccgtactgt gctttgacgc gcaacggctg caattgacta tggtgcagca
    53461 gggtctgcag atcgtttgcg ccgagcggca cggtacgcgg ggcaaggata cccgcgtctt
    53521 tatcgtggcc cgcaagccgg gctgaaccgc ccccagtcac gcgcatgtgg cccgttgcaa
    53581 ggccgcaggg gcagttttgc cttctgggtt tctgctgcgc tgttgccgtc atctttcctg
    53641 gccaccactc aaccccggtt gacatttctc tgccgatccc caaggagccc gtcatgtctc
    53701 tcttctcccc tacgctgcac tccaccaacc gatctggctg gtctggcaat gtgctgatcg
    53761 ccatggccat cgtttcactg acagcttgcg gtggtggctc ggacggcgag agtggcccac
    53821 cagacggcgg ttcgggtggc acggggcctt ccgtgtcgat cggcgcattg gagggggatt
    53881 ggttgcagaa aggatgcgtg aaggccggcg cacaatccta caagcggacc ctgcgcgtgc
    53941 gcatcacagg ccagaccatc gttgactatt tcgaaggcgt gctgaccttc cgtggcaatg
    54001 actgtgctgg cgcatcgcaa gctgctgggc caaccagaac cggcacggtg acttttgccc
    54061 gctcggaagg ttcccaagta ctgaccgctc attggggcga gttacgcacc gtcatcggca
    54121 cccgctcagg agccatctgg acgctgcaac cccaagacct gctgtgcata ttgggcgacg
    54181 agattccaac cattcagccc acgctcacag ccgtgtcctc cagtgtggcc accatccctg
    54241 tagacaactg ctttggccgc tgacaccgcc cacgccatga catccattgc atccaacact
    54301 gccatgaaac cgcgcatgcg cctgctgtcc atcgccccta tggcctgcgt tgtcgcagcc
    54361 caggtgctgc tcccaggttg cgcctccacg cttgatcagg caggtctgga gcagcgcacg
    54421 gcgcaagcca tagggcgttc tgccggccag ttcaccatca cagaccaaca ggaggaaatt
    54481 ggtggccgcg tcagctacac cgtcagcacc agtgagggcg caacctaccg gtgctatctc
    54541 tacggcgcca cgggtttcca aaaggccatg agctttggtc aaacgcccca ttccgatgcg
    54601 atatgcaccg acatggtcgg cagcaagccc agcagcaaac accaggcacc agcgaccgca
    54661 ccagagcagc cgggacgcaa ccgcgccaag gcatgcaatg ctctgcagcg cactgcgggg
    54721 cgttgctgaa taccgctgtg ctgacactcg atccgatcca tgttcaacct tctgaaaccg
    54781 gatcaatggg ggaaatggcc ttgcgtcgtc ctggtactga cactgggctt atgcggctgc
    54841 acgacagtgc cgggcgccgc gatcgaaacg gtggacgggc gcaaggtgga gtacgcgcaa
    54901 tctgggcagg gcacaccggt ggtcgtgttt gagaacgggc tgggtgccac gctcgactgg
    54961 tgggcaaagg tttggcctga tgcagtggcc gaagcacgtt ccttggccta taaccgggcc
    55021 ggttatggcc acagcgatac gtccctggct ccgcgtgacg gcgctcaagt agtggatgag
    55081 ttgcgagcat tgctgcaggc gcgcaatctg ccgccaccgt atgttcttgt gggacattct
    55141 ctgggcggcc tgtacatgca actgttcgca cgcaaattcc ccgacgaggt ggcggcgctc
    55201 gtgctggtgg actccactca tcctgatcag ttcaagggtg ctggcaaccc ggatcagtgg
    55261 cccacttggc tgaaagtggt tctccacgca acgacatccg aaacggcaaa gcaggaactt
    55321 gcggcactgg atgccacagg agagcaagta tccggcttgc cagtggctcc ctcgatgccc
    55381 gtatgggtgc tgagtgcgct gcgcgccaca ggaacgcctt cagcgctgac ggacgacgcg
    55441 gacagaaagc ggtccaacat tgcccgtctg taccccggct ctgtccagat atgggtgaac
    55501 agcggccacg ctattcctct ggaaaaaccc ggggccgtgg tcagcgcgat acgggctgcg
    55561 gcgcgcgcga cgcgcgcatc cggcgctgcg cgcatggaac cgcaagatcg gtagcctaca
    55621 gtcgaggcaa gggtgccctg cattgtgagg gccacgctga aaaagtacat cactgaccgg
    55681 ccagatgctt tatgagtgga tgaagcttcg gaccctcgcc gcaggtgccg gccacgacag
    55741 ccagttcgag caatacctcc gcccgagcaa gcccggcatg ttcctggcga cgacggaaca
    55801 gatcgtgcca tggccggagc tgtgcgcagt caccgagccg cactacccca tgccggccaa
    55861 tggacgcccg ccggcagagc tcgagcgcat gctgcgcacg tgccttgcac agctccggat
    55921 cagcccggtc gatgagcctt gcgaggaggc gctgatggac ggcacgctgg tacaggcgcg
    55981 agggctgaag gcgggcacac ccgtgccgtg atcggcttgc ccggcagcat gaatgcggcc
    56041 tgcccgaagt tgacaagcgg tcaactgccg tttctggggt gagccgttca ccctgagcct
    56101 atcgaagggc gtcggcaggc tcagcccgaa cggtattcaa gacctccaaa ggccgaatca
    56161 ttcggcccag gccggcgcct gcccggcacg ccacagccgc ggcagctcgg ccatggcacc
    56221 gaacagcgcg tgcgcgcccg cctcgcgcag cgccggcgcc gcctcgggct gcgggcacaa
    56281 ggcccagacg gtggcgcccg cggccacgcc ggcctggatg ccggtgggcg tgtcctcgat
    56341 caccaggcag cgcgccggcg gtaccgccag ggcggcggcg gccgccaggt agacgtcggg
    56401 aaagggcttg ctgcgcggca gctcgtggcc gctgaacacg tgcgggccga accatggcgc
    56461 caggccggtg tgggccagtt gcagcaccag cttgccgcgg tcggcgcccg aggcgcaggc
    56521 gatgcgcccg ccggtcagcg cgtgggcggc gcgcacggcg tcgggcgcgc cgtcgatggg
    56581 ctgcaggtgc tggcgcaact gggcgtcgcg ccgggcgtag aagcgggcca tccaggcgtc
    56641 ggtgagcggc cggccggtgc gcgcctcgat cagcgccgcc tggctgcgca cggtgcgccc
    56701 catgaagtgc cgcatgcact cggccagcgg catgtcccag ccgctttcgg ccagttgctg
    56761 gcgcagcacg ccgtgggtga ttggctcgct gtccaccagc acgccgtcgc agtcgaacag
    56821 aatcgcttca aatctcatag ctgtttgcgc acgtccatgg cgggttggat cgacatttct
    56881 ttcatggtcc ggaggctcga acggcgtgcg cagcccgtcc acccagggaa gcggcgcagc
    56941 ggctctgggg ccctctacct gcgccagaac tgcggcgtga acagcaccag caccgacagc
    57001 agctccagcc gccccagcat catggccagg ctgcacagcc agatctggaa atcggtcagc
    57061 accgcgtagt tgccggccgg ccccaccttg cccatgccgg ggccgatgtt gttcaggcag
    57121 gcgatgatgg ccgacaccgc ggtcaccacg tccagcccgc tggccagcat cagcagcgac
    57181 agcccgacca gcacggcgcc atagaccagc atgtaggcga tgacggcgcc gatcacgcgc
    57241 gccggtaccg tctggccacc gagcgtgacc gggttgacca cgcgcgggtg gatcagctgc
    57301 accagctcgt gcagcgtcac cttggccagg atcagcacgc gcaccatctt gatgccgcca
    57361 ccggtagagc cggcgcagga gacgatggcg cccagcagca gcatcagcac cgggccgatg
    57421 gtgggccaca gcaggtagtc ggtggtggcg tagccggtgg tggtggccac cgacaccacc
    57481 tggaacgccg cgtcgcgcag cgcatggccg gcgtccacat gtttgccctc gcgcagcagc
    57541 agcagccaga ccgcggcgac ggacaccagc agcaccacga cgtaggcgcg cacttcggcg
    57601 tcgcgccaca acggtcgcac cgagcgcgtg cgccagacca tgaagtagcg cgcaaagctg
    57661 atgcccgaca gcgccatgaa cagcaccgcc agccactcca gcgcgggcga gttccagtag
    57721 ccgaagctct ggtcgtgcga agacagcccg cccagcccca ccgtggtgca catgtgcatg
    57781 aaggcgtcgt cccagctcat gccaccccag cggtaggcca gcatgcaggc cagcgacagc
    57841 acgaagtaca cgccccacag gccgcgcgcg gtttcggcga tgcgcggcgt cagccgggtg
    57901 tccttcatgg ggccgggcat ttcggccttg aacagctgca tgccgcccag ccccagaaac
    57961 ggcagcaccg ccaccgccag cagcatgatg cccaggccgc cgatcagctg cagaaagcag
    58021 cgccacatgt tgaccgccgg gtcgagcaga tccagccccg ccagcgcggt ggcgccggtg
    58081 gcggtcaggc cgctcatggc ctcgaagtag gcgtcggtga cgctgatgcc gggcacggtc
    58141 agcagcagcg gtgcggcggc aaagcccggc agcaccgtcc acaccagatt gaccagcaga
    58201 aaaccgtcct tgaccgacag ctcgcgccgg aagcgccgcg tggccgccca cagcagcgcg
    58261 ccacaggccg ccgtgccggc accggcgagc agccagatct cggcctggcg gtaacgcgcg
    58321 tccaccagcg tccaggccgc cggcaccaga aacgccagag aaaaacccat gatcagccac
    58381 gccagcaccc gcagcacgtg cacgtggcga cgcatcagca tccgcatgtc cgcagctttc
    58441 agaagaaggt ggcgcgcacc tgaaacagct tttccacctg cggcagcaga cgcttgtggg
    58501 gcacgaagat gatgacgtgg tcgccctcct cgatcaccgt gtcgtggtgc ggcaccagca
    58561 cctggccggc gtcgccctcg ccgcgcacga tggcgcccac gcgtgcgccg gccggcagcg
    58621 ccagctgctc gatgcgccgc cccaccaggc gcgaggtgcg cgcatcgcca tgggccacgc
    58681 cctccagcgc ttcggccacg ccgcggcgca ggctgtgcac cgccgccacc gcgccgcgcc
    58741 gcacatgcgc cagcagctcg ccgatcaccg cgtcggcggg cgagatgatg atgtcgatgg
    58801 ggctggcctg catcatctcg gcgtaggcgc ggcggttgat cagcgccatc acgcggcgcg
    58861 cgcccatgcg cttggccagc atggcggaca tgatgttgtc ctcgtcgtcg ctggtcagcg
    58921 agatgaacag gtccatgtcg cccacgtgct cggcggccag cagctcctcg tcggcggcgt
    58981 cgccctgcag caccagcatg tcgccgggca gctcgccggc caggtagcgg cagcggccgc
    59041 gctgcttctc gatcagcttg acctcgcact ggccgatcag cgagcgcgcc acgcccagcc
    59101 cgaccttgcc gccgccggcg atcatcacgc gcctgaccgg cggctcgcgc gggccgatgg
    59161 cggccagcgc ggcctggaca tgctcgcgcg ccaccagcac gaacacctcg tcgccggcca
    59221 gcacgcgggt gtcggcctcg cagggggttt cttcgtcgcg ccggtacacc gccaccaggc
    59281 gcatcggcac ctcgggcacc tgggcgatga agtcggcgat gctgcggccg tcaagcgcgc
    59341 tgccgcgcgc cacgcgcgcc gccaccaggt gcgcgcgccc ctgcgagaac tgcagcacct
    59401 gcagcgcctc ggggtgttcg atcagctcct ggatggtgcg cgtgacggac gcctcgggac
    59461 agatcacgcg gctgacggca aagccttccg gtcccagcag gcgatcgtcc tggcggaagt
    59521 cgggcaggcg cagccgcgcg atggtggtgg ggatgccgaa cacgtcgtgc gccaccttgc
    59581 aggccaccag gttggtggcg tccaggtcgg cgcaggcgat gaacaggtcg cagtcgtcgg
    59641 cgccggcgtg gcgcagcacc gagggctgaa cgccgttgcc ggccacgccg cgcagctcca
    59701 gccgctcctc cagcccgcgc aggcgctcgg ggtcggggtc gatgatggtg atgtcgttgg
    59761 cctccgaggc cagcgtctcg gccacgctct gccccacgcg gccggcgcca agaatcagga
    59821 tcttcatcgg gggcgacctc cgaacgatcg gccaatgctc atatttttat agctgcttgc
    59881 gctttcagga cgggcgccag agggcggaaa cacctaggac agatcaccat ccaggtaaaa
    59941 ccagcgccag ccgccctgga cattctcgcg cacgaaacgg ctgcgctcgt gcagccgcgt
    60001 ggcgcgcccg ccggcgacgc gcgagcgcgc gacgaactcg acctcggcgt gcgtttcgtc
    60061 cagcacccgg tgcgcgcgca cctccaggcc cagccatcgg atgtgcggct ccagcgccag
    60121 cgtggccggg cgctggctgg cgtgccagct ggacagcagg tagtcggcgt cgccgcgcac
    60181 gaaggcggtg tagcgcgagc gcatcagcgc ctcggcgtcg ggcgctggcg tgtcggccca
    60241 gtgatcgagg tagcgcccgc agcaggcttc ataaggcgtg ccgggcgggc ggccgccgca
    60301 ggggcagggc gtgtcagctt gatgcgctcg cggcaccgcg attctccgca agcatctggg
    60361 cagatttagc gaatgcagcc gcaagtgtct cacgaaagat tgcgattaac tcctgcagcg
    60421 atgagagcat catgtggtga tcagcatgga tgcccttgcc tctgtgtgtc aaattgcttc
    60481 tgacttgata ataatattta atagactttt ttggattgcc gggatctagt ttgtacgatt
    60541 cttctggatt atcagctcga tttaccgttc ggaagtttcc atctggctcg gatttgacgt
    60601 attttcgtaa tgcattttgg aatgccattt ccgtcgatat ttcttcaact ttctggtttg
    60661 cattcctgta aaggtgatac ctcaatgagg cgtagcgttc aatggctgtc cataataaaa
    60721 gatacgccat ttcaagccga aatagtggtt ttaaatccca tgtactttgg tgattctgta
    60781 tatcatccaa gacttcatca attacttcta gagcggaggt aaaaagtgga tcgtttttac
    60841 cagtccatgg ctcttcaagt tccttgctgc cctttcttgg tttcaggccg gctagaatgt
    60901 tggcggtttg accgcctatt gattgaatgg gtttccattc atattgtttg tctggctcca
    60961 gttcgacgat ttttttgtgg gcttcctctt gcgtgccctc acgaaagaat atcaagaaac
    61021 catcaatctg tgagttggta gatgcaagat cggcaattgg caagccgtcg cgaatccgca
    61081 atgtgcccgg aacaccatcc tgctcgcatc tgtcaacaaa ctctcgaatt ttcaaaaaag
    61141 cgagctcgcc cggtctgaac gcaccatagg cgaagaatgg gatctcagga tttgttggaa
    61201 tttccataag caaactcgat acgcgatcgc tcttcagcaa ggctacagcg cgcgcgtgat
    61261 gatgatcttc tgcacgtcgc tggtgccttc gtagatctg
//
LOCUS       DAOQTZ010000003        60010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_569_length_60010_cov_13.481945, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000003 DAOQTZ010000000
VERSION     DAOQTZ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 60010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60010
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_569_length_60010_cov_13.481945"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKN51_00650"
     CDS             <1..365
                     /locus_tag="PKN51_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNT83634.1"
                     /translation="QYPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLAAAV
                     VAKPDPKWGETPCAFLELKPGAEVSEADIVAHCKQHLAGFKVPRAIVFGELPKTSTGK
                     IQKFELRKQAGSAAAIDV"
     gene            complement(465..833)
                     /locus_tag="PKN51_00655"
     CDS             complement(465..833)
                     /locus_tag="PKN51_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HNT83635.1"
                     /translation="MSADALPQWLQWLIAGLLLASGVFALAAAWGLVRLSSFYQRMHP
                     PALVYVGSSWCVTLASILFFSGQQQGPQLHVWIIIILLSITVPITTVLLSRAALFRHR
                     VAGRTDVPEALRPQAPAPQD"
     gene            complement(830..1108)
                     /locus_tag="PKN51_00660"
     CDS             complement(830..1108)
                     /locus_tag="PKN51_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HNT83636.1"
                     /translation="MSPLLSGALALTLGAYAVAMLIALWRVLRGPTAQDRVLALDMIY
                     VIAMLIMLVLGIRHASDLYFEAALLIALFGFVGSAAMAKFLLRGEVIE"
     gene            complement(1105..1596)
                     /locus_tag="PKN51_00665"
     CDS             complement(1105..1596)
                     /locus_tag="PKN51_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNT83637.1"
                     /translation="MRRWLPAPLLTAALILLWLVLNRPFSLGQLPLALLFGVAAPALL
                     APLRPARPRIRHPLKVLRLILTVGHDVLVSSLDVFWTLVRNRSRPVHSRFVVIPLTLR
                     DPTALASLTMITTVVPGTVWCELARDCSAMLLHVWHVPDEAEFVAFFKRRYEQPLKEI
                     FES"
     gene            complement(1593..3302)
                     /locus_tag="PKN51_00670"
     CDS             complement(1593..3302)
                     /locus_tag="PKN51_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HNT83638.1"
                     /translation="MNLSAWIESLMPHLVVAPILLPMLTAALMLLLGDTRRPLKAALN
                     VLASAAGVLVAAALVLQVQRSGAAQSIGVYLPGNWPVPYGIVLVADRLSAMMLLLGSL
                     LGLAGVLYASARWHRAGVHFHPLYQLQLMGLAGAFLTADLFNLFVFFEIMLTASYGLL
                     LHGSGWPRVRAGLHYIAINLMASSLFLIGVAMLYGITGTLNMADMAIIVPTIAEDDRG
                     LLHAGAAILAIAFLVKAAAWPFNAWLPAAYASAAAPVAALFAVMTKLGVYAILRLWTL
                     LFPQDAGISALFGGPVLIGLGMLTLGFGVIGVIAVQQLGRMAAFCAIVSSGTLLAAIG
                     FGRPAVTGGALYYALGSTLAVGAFFLLVELIDRARDVEEQPVYVGESDPDDEPFAFPV
                     DLVPTHGVNLDDDEEALIGRAIPAAMAFLGLAFIVCALTIAGLPPMAGFVGKVVMLSA
                     LLNPAGFGVQAPVSPAAWLLLALLVTGGLLTIIAMSRAGIRCFWAPAGRPPPRLRIIE
                     TLPIALLLLPIGVLVWQADAVLRYANATARHLHDPRDYIGSVMAARPQPSPHASDQGG
                     LRR"
     gene            complement(3299..3691)
                     /locus_tag="PKN51_00675"
     CDS             complement(3299..3691)
                     /locus_tag="PKN51_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HNT83639.1"
                     /translation="MEVILALAIGVLGGCGVWLVLRPRTFQVLMGLSLLSYAVNLFIF
                     AMGSLFEDKPPIVQPGLTPSLEHYTDPMPQALVLTAIVIGFATSALFLVVLMAARGLA
                     DNDHVDGDNVDDNTALPLLRDDAGGPPR"
     gene            complement(3693..6620)
                     /locus_tag="PKN51_00680"
     CDS             complement(3693..6620)
                     /locus_tag="PKN51_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HNT83640.1"
                     /translation="MDLIYLLLLPFAGSLVAALLPTHARNAAAGWAALVGAAALVWVA
                     TRYPALQGGAVLRQELRWLPSAGIDFSLRMDGFAWMFALLVTGIGTLVALYARYYMSR
                     EDPVPRFFSFFLAFMGAMLGVVLSGNVVQLAFFWELTSLFSFLLIGYWHHRKDARRGA
                     RMALTVTGAGGLALLAGVLLLGHIAGSYRLDDILGAGEQIRAHRLYVPMMLLVLLGAL
                     TKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGVFLLARLWPALAGSEAWFWIVAS
                     AGLLTLLLGALLAMFQNDLKGLLAYSTISHLGLITLLLGLGSELAAVAAVFHIMNHAT
                     FKASLFMAVGIIDHETGTRDLRRLSGLWQAMPITGTLAFVASAAMAGVPLLNGFLSKE
                     MFFAETVHLSRNPTLDIGLPVVATLAGVFAVVYSLRFAVGVFLGPPARDLPLTPHEPS
                     RWMRAPVEVLVLVCLLVGVLPQWAIGAALELAARPVVGGAMPAYSLAIWHGINKPLLM
                     SLIALVGGVAMFLWGRQRLAAGDLRLLALPRRLDGQRLFQRLLLGLDRASRGLLRRLS
                     TPRLQPQLLVVVLAAIVLGAAALWPTGISWGERERVPGSAAFALLWVLGGLAALGTAH
                     QAKFHRLAALIMLGAVGLVVSLSFVWLSAPDLALTQLTVEVVTTILFLLGLRWLPKRM
                     PQPGQRLHPRVRARRLRDLLVAGVAGTGMAALAYAMMTRPAPQSIAPFFIDRALPEGG
                     GNNVVNVMLVDFRAFDTLGEITVLGIVALTVYALLRRFRPPREMATLPPQQRAIASDL
                     VSDLINPRTAQDTALGYMLVPAVLVRLLLPVAAVIAMHLFMRGHNQPGGGFVAGLVVA
                     IAFIAQYIVAGTLWVEAHLQLKVIRWIALGLLLAALTGLGSLPLGFPLLTTHTAHLHL
                     PLLGDVHLPSALFFDLGVFAVVVGATLLILTALAHQSVRAYRQAPETMATDAPAHRLP
                     EVG"
     gene            6793..7470
                     /locus_tag="PKN51_00685"
     CDS             6793..7470
                     /locus_tag="PKN51_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT83641.1"
                     /translation="MRMLLVEDDAMIGEVVLDALRAEHHAVDWVRDGAMALTALATAP
                     YDLLLLDLGLPRQDGLSVLRQLRADHQRLPVLIATARDAVADRVAGLDAGADDYIVKP
                     YDLDELLARVRALLRRADGRAEPAYEWGEVRIVPSTREVTLRGQPVQLSAREWAVLEP
                     LLARPGRVLSRQQLEDKLYAWRDEVSSNAVEVYIHGVRKKLGAGLIENVRGLGYLVPV
                     APPESGS"
     gene            7530..8867
                     /locus_tag="PKN51_00690"
     CDS             7530..8867
                     /locus_tag="PKN51_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT83642.1"
                     /translation="MKTPAPSLRARLTALLLLAVLLTAALQAAITWRTALAETEAVFD
                     AQMERIALSLTGGLAASVLGDDALAATRGAEELIIQVWRADGIMLYRSPSARLLPPQT
                     VIGFSHLRAGDEDWRVYALETPSQVIQIAQAERARRRIAGQLALRAVLPVALLAPLLM
                     LIVWWVVGRALAPIERLRRQLAARGAADLAPLPTGGLPAEVLPLVTGMNGLLARLAAA
                     WQQLADFTADAAHELRSPLAALRLQVQSLQRADTEQARAVATGRLLAGVDRATRLVEQ
                     LLALARHDGDADAVREPVDLPVLARQALQEADAEAQARGIALRLEGDASLWLDARPQA
                     LAVLLRNLIDNALRHTPAGGQVCVAVQRRPDGVAELAVEDSGPGIPAAERQRVLDRFY
                     RVPGAAGHGSGLGLAIVQAVARRHGAQVVVQASPRLGGARLAVIWPAAAGPED"
     gene            9027..10517
                     /locus_tag="PKN51_00695"
     CDS             9027..10517
                     /locus_tag="PKN51_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNT83643.1"
                     /translation="MNRLTLKSSRLFAALLAAGVIGGAGVAAVQELTPAQAQPSAAVQ
                     AVAPAAVATPDFAAIAARYGGAVVNISTRGVQPVADEGADSGPGARIDPGLQDFLRRF
                     GLPPGAFQFAPPGPGGPGAVPMHGQGSGFIVSPDGLILTNAHVVRNARTVNVKLTDRR
                     EFQAKVLGRDDKTDVAVLKIDATGLPTVPLGSSQALRPGDWVLAIGSPFGFENTVTVG
                     VVSAKGRSLPDDSAVPFIQTDVAVNPGNSGGPLFNARGEVVGINSQIYSRSGGYQGVS
                     FAIPIELARHVEQQLVAHGKVEHARLGVTIQSVNQALADSFKLPKPEGALVAQVEPGS
                     AAERAGLKAGDVILRANGQAIVDSGDLPARIALARPGDKLALEVWRQGRPEQLSATLQ
                     GARPADTALAQGSAASHGKLGLSLRPLQADEKRQTRLDEGLVVEQASGAAALAGVQPG
                     DVVLSINGQPASSVEKLRAVLAKADKSVALLIERDGQQLFVPVPLA"
     gene            complement(10811..11260)
                     /gene="comJ"
                     /locus_tag="PKN51_00700"
     CDS             complement(10811..11260)
                     /gene="comJ"
                     /locus_tag="PKN51_00700"
                     /inference="COORDINATES: protein motif:HMM:NF022486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComJ"
                     /protein_id="HNT83644.1"
                     /translation="MSIDHGQVVVHGEGEPGAGLLWTDEHVAQGFAWSEKLLTLGVPD
                     HDGECRIQVELVPEATVSAQALWAVQMPLEVTQPLHIGALFERHRVVVPNGRYALLYQ
                     ALPGTQGEAYVLRLSLAATPQPAFRILRTGGDVTADAVLRRDAQLLG"
     gene            complement(11421..11690)
                     /locus_tag="PKN51_00705"
     CDS             complement(11421..11690)
                     /locus_tag="PKN51_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83645.1"
                     /translation="MPLPTRRLAAIALLATLIASPTLAQAKKEGAGKTPRSGAGSRGV
                     RLKPSPTATYASGESPAERQRNEDARLRRECRGRPNAGACLGYTR"
     gene            11775..12950
                     /locus_tag="PKN51_00710"
     CDS             11775..12950
                     /locus_tag="PKN51_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT83646.1"
                     /translation="MRQAVLNLEESKIREVANAGMGRADVLPFWFGESDEVTPEAVRA
                     AGIASLQAGETFYSHNLGLPELREAVAAYASRLHGELAPQRLAITSGGVNALIVAMQA
                     LIDAGDEVLAVTPVWPNLTAQPRILGAQLRCVALQPDAEGAWQLDVPALLAAITPRTR
                     LLVLNSPNNPTGWTLTRAEQQAILAHCRQTGTWILSDEVYERLYFRDDTANGAAPSFL
                     DIAAPDDRLVVVQSFSKAFLMTGWRLGWLVMPPAMTPQVGKLLEFNTSCAPVFVQRAA
                     LVALAQTEAITPRIVAHLRHCRDTLVPLLQAVPGVQVGRAPGGMYAFLRLVGQNDSLQ
                     LAKRLVQEAGLGLAPGVAFAPQAEGWLRWCFASRDVARLRQGVDRLHHWLRTAAPAP"
     gene            13001..13963
                     /locus_tag="PKN51_00715"
     CDS             13001..13963
                     /locus_tag="PKN51_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNT83647.1"
                     /translation="MSRRLRLAQLDLFEKVLETGSILAASRELAITQPAVSKSLRELE
                     QQLGGALFVRGKHGVTLTELGQLFARHARAMTSGLQDMADELNAWHGGTAGRVVVGAM
                     LSATVALLPEAISRLRDMAPDVLVTIRVGPNSVLFPALARGEIDLMVGYLPANASAAT
                     QDAAPGTRLVHVPLYDEGMSVVVGPDHPLAHRTDVRVAELHRFEWIVPTPDAVAYPAV
                     RALFEREGLSLPRSRVESISILTNVGLLARRPMVALMPHSVATRLASAGLVVVLPLPS
                     LGSFSALGYTRRAEQGMNMALQHLIDALHAVGDALQRGAGLDLS"
     gene            14107..15594
                     /locus_tag="PKN51_00720"
     CDS             14107..15594
                     /locus_tag="PKN51_00720"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HNT83648.1"
                     /translation="MGRSFALPATLGSLRNYVDGEFVETGRTFDNVSPIDGSVLAKVH
                     EADAALVDRAVRAARRALDEGPWGRTRVEQRAEALHRVADIIARRFDEFLQAEVADTG
                     RPVEQARKLDVYRGIANFRSFADLGKTAHGEFYETHLPDGSELINYTKRKPLGVVASI
                     SPWNLPILSLTWKAAPALICGNTIVCKPSEETPSSAVLIAEAFHEAGVPPGVFNVVHG
                     FGPGSAGEALTTHPGIDAVTFTGESRTGAAIMQAVAPGVKEVSFELGGKNAAIVFADC
                     DFEPTVEGMARALFTNTGQVCLCPERVFVERSIFERFVAALKAKTESLKIGWPDEPDV
                     AMGSLISREHREKVLSYFRLAVEEGATVVTGGGVPKFGDARDNGAFVQPTLWTGLPDS
                     ARCVREEVFGPVCHLSPFDSEDEVLRRANDSDYGLAATVWTSNLSRAHRVAPQLHVGI
                     VWVNTWFSRDLRTPFGGTRLSGIGREGGRWSLDFYSETTNICIKV"
     gene            15627..16880
                     /gene="kynU"
                     /locus_tag="PKN51_00725"
     CDS             15627..16880
                     /gene="kynU"
                     /locus_tag="PKN51_00725"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNT83649.1"
                     /translation="MPITLQQCQTWDQADELAALRDEFDLPAGTIYLDGNSLGAMPRN
                     ALARAQDIIARQWGVDLINSWNKAGWWQLPTRLGDQLAPLVGAGAGEIVVTDTTSLNL
                     YKVLANAVRIQQRRQPERKVILAERDAFPTDLYMVQGLLGFMDRGYTLELIDRPEDLP
                     SRLGEQTAVVLLSHVNYRTGYQWDLSAASQQAHAAGALIVWDLCHSIGAVPIDLEAAD
                     ADFAVGCTYKYLNGGPGSPAMLWAKPRHRADFWQPLSGWWGHARPFDMAVDYAPDRGI
                     RGFLCGTQPIVSLGLVACGLEIFAKTDMHKLRRKSLALTDLFIALVEQECAGQGLTLV
                     TPREHAQRGSHVSFRHPEGYAVVQALIARGVIGDYREPEVARFGVTPLYLRFVDIWDA
                     VQHLKAVLTQREWDQPRYRQRGAVT"
     gene            16906..17361
                     /locus_tag="PKN51_00730"
     CDS             16906..17361
                     /locus_tag="PKN51_00730"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNT83650.1"
                     /translation="MTATFNRSTQASVVAGKATPRGRFPHIKRAGDFLFVSGTSSRRP
                     DNSFAGVQVDALGVTNLDIRAQTRAVIENIRDILLSQGAQLSDLVEAQVFLVNMNDFG
                     GFNETWAEFFDYEGPTRTTVAVHQLPHPHLLIEIKATAYHPRPGTGQPR"
     gene            17373..17936
                     /gene="nbaC"
                     /locus_tag="PKN51_00735"
     CDS             17373..17936
                     /gene="nbaC"
                     /locus_tag="PKN51_00735"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HNT83651.1"
                     /translation="MQPKYGLPKNLMDWVGAHSDRLRPPVCNAALFPDGNYIINMVGG
                     GNTRTDFHDNPTEEIFYQIRGTAYLNIWDRGKFDRIDLKEGDVWLMEPHLQHSPQRPD
                     PHGLCFLVELKRPQGAIDTLNWYCPRCAALVWGASMELKDLVADLPATYQKFYALSDA
                     ERTCPHCGTVHPGKDYAAWHAIREAHA"
     gene            17933..18952
                     /locus_tag="PKN51_00740"
     CDS             17933..18952
                     /locus_tag="PKN51_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT83652.1"
                     /translation="MSGALSMIDIHAHFLPQIGQQEAAAVERERAPWLRIDAGGETGQ
                     IMVGEREFRPVYRALWDADFRVAELDAQGIALQLVCATPIMFGYAWEAGRAADWAAQM
                     NDKALSFCARHPTRLKALAQVPLQDTARACAEASRAMAAGCVGVQIGNHVGDKDLDHP
                     DLVDFLIHCADHRIPVLVHPWDMMGGPGRMKQWMLPWLVSMAAETQLGMMSLILSGAL
                     ERIPTSLRICFAHGGGSFAYLLGRADNAWRQRDIVRKDCPRPPSEYARRLHTDAIVFD
                     PRALHLLIDVMGVDNVMLGSDYPFPLGEQQVGSLVRGVDFLSGSQRAQILSDNARRFF
                     ALPEG"
     gene            19001..20164
                     /locus_tag="PKN51_00745"
     CDS             19001..20164
                     /locus_tag="PKN51_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT83653.1"
                     /translation="MKSTVRNVIAAAALSMLASHALADVKIGFMATMSGSAAALGQDM
                     YDGFMLALEQNGGKLGGQKIDLIKEDDQLKPDVGVQIVQKFIEKDKVDIVAGVTFSNV
                     MMAIAKPLADADMTYVGSNAGPAPLAGKQCSKGFFFASWQNDQQAEVMGAFANDKGYK
                     KAYLLAPNYQSGRDQIAGFKRQYKGSVVGEVYTQLNQPDYSAELAQLQAASPDVVYVF
                     YPGGMGVNFIKQYRQAGLLGKVPLVSASTVDGTTLPALKEIALGAVTGTFWGPDFNNA
                     TSKAFVAAFEKKYNRIPSQYAAQAYDSALLIDSALKKTGGKVADKQALRTALRAADYA
                     SLRGPFKFDVNGFPIQDFHVFEVAKDGAGRVSLKTIATPLKAHRDAYVGECKL"
     gene            20418..21338
                     /locus_tag="PKN51_00750"
     CDS             20418..21338
                     /locus_tag="PKN51_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT83654.1"
                     /translation="MTPELLLIQTLNGLQFGVLLFLMAAGLTLVFGIMSFVNLAHGAL
                     YMFGAYFAAAAWNASGSFLLAVLGGFGGAFVLGALVDRVGLFALYPRDHLDHVLATFG
                     LVLFANELARMIWGASPVFMELPESLAASVPLLGINYPVYRMLIIVVGLAVAACLYLL
                     IERTRVGMLIRAGASNRAMVSALGVNIGWLNTFIFALGAGLAGLAGAMVGPIMSVQPG
                     MGDPILITTLVVIVIGGIGSIRGAFLGALVVGVVDTVGRTLVPALLRGALAPEMANTL
                     GPALSSLAVYVLMAVVLALKPDGLFPVRKR"
     gene            21452..22582
                     /locus_tag="PKN51_00755"
     CDS             21452..22582
                     /locus_tag="PKN51_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT83655.1"
                     /translation="MASATPCGRRPAASPRVDGRADHGAGGRAQRLVPGLKGRNMVSS
                     SVGVHAADRLAPTLLGLSKARWRTVLTVLLAAFFVLVPLYAWLTGDSYVLILMGRILV
                     FALAAVGLNLALGYGGMVSFGHAMYLGIGAYVVAIASHHGLDNGLVHLLLVAALTVAV
                     AIPVGLIALRTQGIAFIMITLAFAQMLFFVAVGLKQYGGDEGLSIVHLSRFGALTGNK
                     TALYLSMLLALGLALWLLRRVVASRFGLVLRATMINERRVRAVGTPPLGYRLAGYVAS
                     ALLCALAGYFLANLTSFASPAYMAWTVSGELIVMVLLGGMGTLVGPVVGALVFLLLEE
                     GLKALTDHWLVIMGPVIVVIVLFLKNGLWGIVSDVPAARGGH"
     gene            22584..23348
                     /locus_tag="PKN51_00760"
     CDS             22584..23348
                     /locus_tag="PKN51_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT83656.1"
                     /translation="MTQSQPLLSLRGLVKRYGGLLVTDHVDLDVLPGEVHAIIGPNGA
                     GKTTLIGQIVGEVASDEGSIRLQGQSIDGLSVPERARAGLGRSYQITSVIGAFTVLEN
                     VILAVQGCLGHAFHFWRPASETAELVERADEVIERVGLSAQRDVHAAALAYGQQRQLE
                     MAMALAMQPSVLLLDEPMAGMGAVESSQLVALLQRLKGQYGILLVEHDMGAVFALADR
                     ISVLFHGKVICCGDPATVKAHPLVREAYLGDEEIPA"
     gene            23351..24049
                     /locus_tag="PKN51_00765"
     CDS             23351..24049
                     /locus_tag="PKN51_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT83657.1"
                     /translation="MLEVEGLAAGYGQSQVLFDIGLAVAPGQCVTLLGRNGMGKTTTI
                     KTIMGLLRPWGGVVRWKGRAVQGRPPHAMARLGLGLVPEGRQVFPNLTVRENLVATGR
                     GGGSSHAWTLARVHALFPRLAERASNFGNQLSGGEQQMLAIGRALMTNPELLILDEAT
                     EGLSPLVRREIWLALRQIKAEGMAILVVDKNLAPLLELAERHHIVEKGQVAWSGSSEE
                     LRDQRSLMQRYLSA"
     gene            24307..24750
                     /gene="speD"
                     /locus_tag="PKN51_00770"
     CDS             24307..24750
                     /gene="speD"
                     /locus_tag="PKN51_00770"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNT83658.1"
                     /translation="MRVLPHTDSPTPVVASGATLRSAHAVARMAPGLHLIGDLYGCRG
                     DTRLMTDAATLEAFCKQAVADAGLTTVGSLFHSFGEGEGVTGAVVLAESHLALHTWPE
                     DNYVTLDVYVCSYTNDNSAKAERLFDALMQAFQPADPHLHRVVRA"
     gene            24759..25718
                     /gene="speE"
                     /locus_tag="PKN51_00775"
     CDS             24759..25718
                     /gene="speE"
                     /locus_tag="PKN51_00775"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNT83659.1"
                     /translation="MSASIGTGGQFAVEQLSPDFGFYLRTTGLLAERRSSMQHIEIVD
                     TPLFGRAMRIDGCFMTSERDEFFYHEPMVHLPAIAHGDPRQALVVGGGDGGAAHHLLR
                     YPNMARVVLAELDRDVIDMARAWLPAVHRGAWDDPRLELRLGDGRAFIESCESRFDQI
                     VLDLTDPFGPAVALYTCEFYRACRRALRPGGVLSLHVQSPIHRGATMARLLASLRSVF
                     PVVRPFLQYVPLYGTLWAMAMASDRADPLALTAAEVDARLARHGLNDLQLYSGDTHLA
                     LLSLPPFVRRLLAEPARPVVDGDSLDDPSLDPGAERTLRLVRG"
     gene            25822..26379
                     /locus_tag="PKN51_00780"
     CDS             25822..26379
                     /locus_tag="PKN51_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNT83660.1"
                     /translation="MKKLLIAVALGLAGWSAGAVETGISAREAYEKVQSNAPGVLFVD
                     VRDPVEIMFIGFTDVVHTNIPFMMVDRSKWDEQRGIFRLYPNPDFVAQIKAELARRGM
                     GMDAEVITLCRSGSERGEPSARLLRENGLPNARYVINGFQGAALKDGPQAGLRIQNGW
                     QNSGLPWSAKMSPGKVYRVDRQPGN"
     gene            26515..26781
                     /gene="rpsO"
                     /locus_tag="PKN51_00785"
     CDS             26515..26781
                     /gene="rpsO"
                     /locus_tag="PKN51_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNT83661.1"
                     /translation="MISKENKAAVVKDNARAANDTGSPEVQVALLTARINELTPHFKE
                     HAKDHHGRRGLLRMVSRRRKLLDYLKAKDADRYLALIAKLGLRK"
     gene            26997..29279
                     /gene="pnp"
                     /locus_tag="PKN51_00790"
     CDS             26997..29279
                     /gene="pnp"
                     /locus_tag="PKN51_00790"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNT83662.1"
                     /translation="MSMFNKITKSFQWGDKTVVMETGEIARQATGAVLVDIDGTVILA
                     TVAASKSAKPGQDFFPLTVDYIEKTYAAGKIPGSFFKREAKPSELETLTSRLIDRPIR
                     PLFPEGFFNEVHVVIHTVSLNPEVDADIAAMIGVSAALSISGIPFNGPIGAARVGYIN
                     GEYVLNPGPTARKSSQLDLVVAGTEAAVLMVESEADQLSEDVMLGAVVFGHQQGQIAI
                     NAIHELVREAGKPLWADNGTWVPEAKDQAFIAQVNAAAEGPLRAAYQIRSKQARTQAC
                     REAYAGVKAALTEQGVAFDPVKLDNLLFEIEARIVRGQILAGEPRIDGRDTRTVRPIE
                     IRNSVLPRTHGSALFTRGETQALVVTTLGTERDAQRIDALAGEFEDRFLFHYNMPPFA
                     TGEVGRMGSTKRREVGHGRLAKRALIPLLPAKDEFPYTIRVVSEITESNGSSSMASVC
                     GGCLSLMDAGVPMKAHVAGIAMGLIKEDNKFAVLTDILGDEDHLGDMDFKVAGTTAGI
                     TALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQEAMGQARTEISSYAPKLYTMKI
                     NPEKIRDVIGKGGATIRALTEETGTTIDIAEDGTITIASTDAAKADHARRRIEEITAE
                     AEVGKVYEGPVTKLLEFGALVNILPGKDGLLHISQIAHERVEKVSDYLQEGQVVKVKV
                     LETDDKGRIKLSMKALLERPAHLEGSDERPPRGDRPPRGERGERRERAPREDRGEREE
                     RPAPAAGAPEAAPELPSPQI"
     gene            29340..30359
                     /locus_tag="PKN51_00795"
     CDS             29340..30359
                     /locus_tag="PKN51_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HNT83663.1"
                     /translation="MKCIEIREPGEPAVLVPAERPLPQAGPGELRIRVSASGVNRPDV
                     LQRAGHYPAPPGASDLPGLEVAGVIESGDAAALSAAGLQLGQRVCALVAGGGYAEVCV
                     APVAQCLPVPAGCSDVEAAALPETFFTVWSNVFDRGRLQAGETLLVQGGTSGIGVTAI
                     QLAKAAGATVIVTAGSDDKCAAALRLGADHAINHRTQDFAQQALQLTGGRGVDVILDM
                     VAGDYVQREVECLAEDGRLVIIAVQGGTRSQFNAGLVLRRRLTITGSTLRPRPVAFKA
                     AIAQALRQRVWPWLEQGRVRPVIHQVWPALEAGDGLPSGAARAHALMESGQLIGKLVL
                     TWSQP"
     gene            30356..31108
                     /gene="tpiA"
                     /locus_tag="PKN51_00800"
     CDS             30356..31108
                     /gene="tpiA"
                     /locus_tag="PKN51_00800"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNT83664.1"
                     /translation="MSERKKLVAGNWKMNGSLAANQALVQALRQGVGQPACDVAVCPP
                     SVYLGQLQQLLAGQTAIALGAQDLSQHEGGAFTGDVSATMLKDFGVRYAIVGHSERRQ
                     HQGETDLQVALKVRRALAAGITPIVCVGETLREREEGLTEFIVKRQLSAVLHLNGHAI
                     NETVVAYEPVWAIGTGQTATPAQAQSVHAVLRAQLRAAGAQAEHVRILYGGSMNAGNA
                     AELLAQPDIDGGLIGGAALKAPDFLTIVAAAA"
     gene            31149..31592
                     /gene="secG"
                     /locus_tag="PKN51_00805"
     CDS             31149..31592
                     /gene="secG"
                     /locus_tag="PKN51_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNT83665.1"
                     /translation="MNVILNLILVVQLLSALAMIGLILVQHGKGADMGTAFGSGASGS
                     LFGATGGANFLSRTTAVLATVFFVCTLALAYFGFSVRSAPADSGSVLDRAAVTAPAAG
                     ASAAAPATAADAIPSGGTPAPATEPSRPATPAAPAASGAGQIPGQ"
     gene            31719..31803
                     /locus_tag="PKN51_00810"
     tRNA            31719..31803
                     /locus_tag="PKN51_00810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31753..31755,aa:Leu,seq:gag)
     gene            31862..32221
                     /locus_tag="PKN51_00815"
     CDS             31862..32221
                     /locus_tag="PKN51_00815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNT83666.1"
                     /translation="MNLAQYLPVLLFILVGLAVGALLLGMGNFLKPHKADAAKNSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVSLREVGLVGFVAVLIFLAI
                     LVVGFVYEWKKGALDWE"
     gene            32261..32740
                     /locus_tag="PKN51_00820"
     CDS             32261..32740
                     /locus_tag="PKN51_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNT83667.1"
                     /translation="MNSEFQQKGFLLTGVDAAVNWAKTGSIWPVTFGLACCAVEMMHA
                     AASRYDIARFGAEVFRASPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGCDRVVPVDVYVPGCPPTAEALLYGIIQLQQKVRRTHTIARV"
     gene            32743..33351
                     /locus_tag="PKN51_00825"
     CDS             32743..33351
                     /locus_tag="PKN51_00825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNT83668.1"
                     /translation="MTKHAIAPEVLNDTVASALGELVQHIDVARGEVTVSVSAGEYHQ
                     VMRLLRDAAGCRFEQLIDLCGVDYSTYGDGRWEGPRFAVVVHLLSVSLNQRLRVRVFC
                     TDDEFPVIRSVTDLWSGADWFEREAFDLYGIVFEGHADLRRILTDYGFIGHPFRKDFP
                     LTGHVEMRYDAERQRVIYEPVTIEPREITPRVIREDNYGGLH"
     gene            33357..34610
                     /locus_tag="PKN51_00830"
     CDS             33357..34610
                     /locus_tag="PKN51_00830"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNT83669.1"
                     /translation="MADIKNYTLNFGPQHPAAHGVLRLVLEMDGEVIHRADPHIGLLH
                     RATEKLAEHKTYIQSLPYMDRLDYVSMMCNEHAYCLAIEKLLGIEVPIRAQYIRVMFA
                     EITRLLNHLMWLGSHGHDCGASTMLLYTFREREDLFDMYEAVSGARMHAAYFRPGGVY
                     RDLPDSMALYKSSKIRSSKSLDAINANRQGSLLDFIDDFTKRFPVLMDEYETLLTDNR
                     IWKQRTVGIGVVTPERALNLSLTGPMLRGSGIAWDLRKTQPYDVYAALDFDIPVGKTG
                     DSYDRYLVRVQEMRESNRIIKQCVDWLRANPGPVIVDNHKVAPPPRERMKSGMEDLIH
                     HFKLFSEGMHVPEGEAYAAVEHPKGEFGIYLISDGANKPYRMKIRAPGFAHLSAFDEL
                     TKGHMLADAVAIIGTMDVVFGEIDR"
     gene            34607..35125
                     /gene="nuoE"
                     /locus_tag="PKN51_00835"
     CDS             34607..35125
                     /gene="nuoE"
                     /locus_tag="PKN51_00835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNT83670.1"
                     /translation="MSSAVAPSTATPFSQATLARFAREVAKYPADQKQSAVMACLAIV
                     QQEQGWVSPDSERAVAAYLGMPVMAVHEVTTFYNMYNQQPVGRFKLNVCTNAPCMFAG
                     GPQALEHLCETLGVQPGETTADGLFTVQACECLGACGDAPVMLVNDRHMCSFMSNDKL
                     DQLVNGLRHAED"
     gene            35129..36475
                     /gene="nuoF"
                     /locus_tag="PKN51_00840"
     CDS             35129..36475
                     /gene="nuoF"
                     /locus_tag="PKN51_00840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNT83671.1"
                     /translation="MARVQDILAQFQARGLETCFHGRHIHPQIYAGLDGTNWGLKDYE
                     ARGGYQALRKVLGQDGGPGMTQDEVIAELKASSLRGRGGAGFPTGLKWSFMPRNFPGQ
                     KYLVCNSDEGEPGTFKDRDILMHNPHAVIEGMAIAAYAMGASVGYNYIHGEIFRVYER
                     FEAAIEQAREAGYLGQGIMGSGFDFQLHAHHGFGAYICGEETALLESLEGKKGQPRFK
                     PPFPASFGLYGKPTTINNTETFAATPWIIRHGGQAYLECGKPNNGGTKIFSIQGDVAR
                     PGNYEIPLGTPFAKLLELAGGVRGGKTLKAVIPGGSSSPVLPADIIMQCTMDYDSIAK
                     AGSMLGSGAVIVMDESRDMVETLRRLSYFYMHESCGQCTPCREGTGWLWRMVDRIQEG
                     QGRAEDLDKLDNVAENIMGRTICALGDAAAMPVRAMIKHFRHEFAAKIDAAQTLTS"
     gene            36499..38613
                     /gene="nuoG"
                     /locus_tag="PKN51_00845"
     CDS             36499..38613
                     /gene="nuoG"
                     /locus_tag="PKN51_00845"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNT83672.1"
                     /translation="MVELEIDGKKVEVPEGSMVMHAAEKADIYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPMPACATPVTQGMVVRTKSDKAIKAQKSVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGASGSRYGDEKRVVAPKNVGPLISMKEMTRCIHCTRCVRFGQEI
                     AGAMELGMSNRGEHSEIETFVGQSVDSELSGNMIDICPVGALTSKPFRFSARTWELSR
                     RKSISPHDSTGANLVVQVKNHQVMRVVPFENEAVNECWIADRDRFSYEALNGEQRLTK
                     PMLKQDGVWREVDWQTALEHVANGLSEIRASHGAGRIGALVSPHSTLEELHLAASLVR
                     GLGSENIDHRLRHSEFPAPEGVRWLGTSVASLSELQSVLVVGSNLRKDHPLFAQRIRQ
                     AARHGCAVAAITSRALLKTDTAWAMPLRAVQAVEAGDWAQALADVATVLAADKGVPAP
                     VAGQATDSARALAGSLQSGERKAILLGNAAAHHAQASVLLALCQWIGEQTGATVGWLT
                     EAANTVGAQVVHAVPGKGGLNAARMLAGDVDALLLLACEPGADSAPLQRQGCGLVVTL
                     SPFKANLDISDVLLPIAPFTETSGTFINAEGRVQSFHAVVRPLGDTRPGWKVLRVLAD
                     LLQVVQPAFETSQEVLAHALGGSVSEFVPAARLSNRTTAAIQIRAGAVHVPPVGIYQL
                     DGIVRRAPSLQQTADALEGASA"
     gene            38610..39686
                     /gene="nuoH"
                     /locus_tag="PKN51_00850"
     CDS             38610..39686
                     /gene="nuoH"
                     /locus_tag="PKN51_00850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNT83673.1"
                     /translation="MIDAIYNVGLHLFAGSWWSGIAWPVVWSLIKIVVLLLPLMGAVA
                     YLTLWERRLLGFMQARLGPNRVGPAGLLQPIADAVKLLTKEIIRPTAAAKGLFFIGPV
                     MAIMPALAAWVVIPFGPEAALANINAGLLLVLAITSIEVYGVIIAGWASNSKYPILGA
                     LRASAQMVSYEIALGFCFLVVLMVSGSMNLTDIVMGQAKGMAADKGLGFLSWNWLPLL
                     PIFMVYLISVVAEVNRHPFDMAEGEAEIVAGHMVEYSGMGFATFFLAEYASMWLVSIL
                     AVLMFLGGWLPPIGALDFIPGWIWLGIKTFIVVSMFIWIRATFPRYRYDQIMRLGWKI
                     FIPVTLIWLIVVGLWMHSPWNIWH"
     gene            39701..40210
                     /gene="nuoI"
                     /locus_tag="PKN51_00855"
     CDS             39701..40210
                     /gene="nuoI"
                     /locus_tag="PKN51_00855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNT83674.1"
                     /translation="MASVTASPFSLRDFLKSFVLVELFKGMALTGRYAFRRKVTIQYP
                     EEKTPLSPRFRGLHALRRYDNGEERCIACKLCEAVCPAMAITIEAGERADGSRRTTRY
                     DIDLTKCIFCGFCEESCPVDSIVETHILEYHGEKRGDLYFTKDMLLAVGDRYESEIAA
                     AKAADARYR"
     gene            40245..40916
                     /locus_tag="PKN51_00860"
     CDS             40245..40916
                     /locus_tag="PKN51_00860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNT83675.1"
                     /translation="MDVKTGFFYVFALVMLFSAFRVITARNPVHAVLYLMLTFSQAAG
                     IWLLLKAEFLGIALVLVYLGAVMVLFLFVVMMLDIHIDTVRQGFWRHVPLALAIGALI
                     TLEMAVVLFGGFRLSEAPQVPETMTNAAGQVVQYSNTKVLGTLLYTKYLYPVEIAAVL
                     LLIAMVAAIALTLRERKDTKKVDPSLQVRVKASDRLQVLKLAPTQAPLPVEPPAAAIV
                     EEVKP"
     gene            40913..41221
                     /gene="nuoK"
                     /locus_tag="PKN51_00865"
     CDS             40913..41221
                     /gene="nuoK"
                     /locus_tag="PKN51_00865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNT83676.1"
                     /translation="MTLTLAHFLTLGAILFSLAVVGIFLNRKNIIVLLMAIELMLLAI
                     NMNFVAFSHYLGDMHGQVFVFFILTVAAAEAAIGLAILMLLFRAKSSINVDEINSLKG
                     "
     gene            41229..43250
                     /gene="nuoL"
                     /locus_tag="PKN51_00870"
     CDS             41229..43250
                     /gene="nuoL"
                     /locus_tag="PKN51_00870"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNT83677.1"
                     /translation="MSQTLSAATLLAVPLAPLAGSLIAGILGTAFGGNRIGRTASHTV
                     TILGVLIAFVLSALTLQSVIAGARFEGTLYEWMVVGGLKMEVGFLIDSLTATMMCVVT
                     FVSLMVHIYTIGYMADDDGYNRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSY
                     LLIGFWFNRPTAIFANLKAFLVNRVGDFGFILGIGLIAAYAGSLNYAEVFGKAGQLAG
                     LGFPGTDWLLVTVICICLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAG
                     IFMVARMSPLFELSDAALNFVLVIGAITALFMGFLGIIQNDIKRVVAYSTLSQLGYMT
                     VALGASAYSVAVFHLMTHAFFKALLFLAAGSVIIGLHHNQDIRWMGGVRKYMPITWIT
                     FLLGSLALIGTPFFSGFYSKDAIIEAVHASKLPGAGFAYFAVLAGVFVTAFYSFRLYF
                     LVFHGQERYDQNPDAHHHDDHHGHGHDAKPHETPWVVWLPLVLLAIPSVVIGAITLMP
                     MLFGDFFRLNDVIHVDAARHPAMAVLAEKIHGWVPMALHGFVAPPFWLALAGVACAYY
                     MYMVNPALPAAIQRAFRPVYSLLENKYYLDWFNENVVARGARLLGTGLWKAGDRALID
                     GLLVNGSWRLVGWVSGMVRWIQSGYLYHYALVMILGIFVLMTYFVWLNK"
     gene            43269..44744
                     /locus_tag="PKN51_00875"
     CDS             43269..44744
                     /locus_tag="PKN51_00875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNT83678.1"
                     /translation="MGWLSLAIWTPIVFGAVLLALGRDSQAQLVRWIALIGSVVSLLI
                     TLPLYTGFRLGTAEMQFVEKMPWISRFNVNYYLGVDGISLWFVLLTAFITVIVVIAGW
                     QVIQRKVNQYMAAFLILSGLMVGVFAALDGVLFYVFFEATLIPMYLIIGIWGGPRKIY
                     AAFKFFLYTLLGSMLMLVAFIYLYAVSGGSFDVLTWHKLPLSATAQTLLFFAMFAAFA
                     VKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFLRFSMPILPDASREWAWLM
                     IALSLIAVIYIGLVALVQQDMKKLVAYSSVAHMGFVTMGFFIFNDLGVTGGLVQMIAH
                     GFVSGAMFLCIGVLYDRVHSREISSYGGVVNSMPRFGAFALLFFMANCGLPGTAGFIG
                     EWMVILATVRANFWLGLLLATALITGAAYSLWMFKRVYFGAVANDDVRAMPDIDGREF
                     LVLALLAIAVLWMGLYPKPFTDVMDASVVNLLQHVANSKLN"
     gene            44755..46242
                     /gene="nuoN"
                     /locus_tag="PKN51_00880"
     CDS             44755..46242
                     /gene="nuoN"
                     /locus_tag="PKN51_00880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNT83679.1"
                     /translation="MIDRFSWMAVYPEIVLLVMACVITLVDLLDRNPRRLAAYVLSIL
                     TLLVLAVLTGTYARGGQTWYGFEGMVVSDPMTNWLKCFAALAVAVSLVYARTYVTGRG
                     MLRGAEWYVLSLLSLLGMYVLISANNFLLVYLGLECMTLSGYALVALRRDHVTSIEAA
                     MKYFVLGAMASGFLLYGLSMLYGATGTLDIGGVFKAVASGQIKHQVLVFGVVFVVAGL
                     AFKFGAVPFHMWLPDVYEGAPTAVTLMIGGAPKLAAFAMAVRLLVEGLLPLAIDWQQM
                     LAVLAVASLLIGNLAAIAQTNLKRMLAYSTIAQMGFVLLGLMAGVVNHSALAAANAYS
                     SAMFYVVAYVLTTLVSFGIILLLSREGFESEEIVDFAGLNQRSPLYAGLMAICLFSLA
                     GIPPMVGFQAKLAVLQALVSTGLPSYIGLAVFAVMMSLVGAFYYLRVVKVMYFDEPLS
                     SAPIQAPFDMRAVLSVNGLLLLVLGIVPGSLMALCAKAVVQMLAT"
     gene            46245..46550
                     /locus_tag="PKN51_00885"
     CDS             46245..46550
                     /locus_tag="PKN51_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HNT83680.1"
                     /translation="MSQNLSIWLLILLALVAANLPFVNHRLMAVLPLRTAAKGLAWRL
                     LELLLWYGFVIGVGMAIERAQGQNQPQGWEFYAVTAALFLTLAFPGFVYRYLVRRGR"
     gene            46550..47143
                     /locus_tag="PKN51_00890"
     CDS             46550..47143
                     /locus_tag="PKN51_00890"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNT83681.1"
                     /translation="MASDLRETRVGGQELFKGDFLHAMRDRVRLPDGQLTQREYIVHP
                     GAVMIVPWLQGPDGSLQLVLERQYRYPVQQTIIEFPAGKLDPGEAPLVCAQRELREET
                     GYTAREWARAGLLHPVVSYSTEFIEVWFARGLQAGERRLDEGEFLEVFTASPEQLYAW
                     CRDGEITDAKTLIGALWLQNVASGAWSLNWQPADPAA"
     gene            47151..47627
                     /locus_tag="PKN51_00895"
     CDS             47151..47627
                     /locus_tag="PKN51_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HNT83682.1"
                     /translation="MKVLNLQCAHGHAFEGWFASEDDFQDQRTRQLVECPLCGDVEVT
                     KLPSAPRLSLSGASAADTASSASATGPTAAAASVANLPPAALQAAWMHLVRQVMARTE
                     DVGERFAEEARRMHYGETEDRGIRGQATPAETEALLEEGIAVMPLPLPPGFKGTLQ"
     gene            complement(47743..48462)
                     /gene="fnr"
                     /locus_tag="PKN51_00900"
     CDS             complement(47743..48462)
                     /gene="fnr"
                     /locus_tag="PKN51_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HNT83683.1"
                     /translation="MTPQAIKVACSNCNLRELCMPMGLSDTEMGKLDDLVATRRKVKR
                     GTSLFANGDRFTALYAIRTGFFKTCVATADGRDQVTGFQMAGEVIGLDGIVGDRHTCD
                     AVALEDAEVCVMPYDRIEELSREVQALQSHVHKIMSREIVREHGVMLLLGSMRAEERL
                     AAFLLNLVQRLHARGFSQSELVLRMTREEIGSYLGLKLETVSRTFSKFAEEGIVEVKQ
                     RHVRILNTEALRAIVNAPSCS"
     gene            48656..50038
                     /gene="hemN"
                     /locus_tag="PKN51_00905"
     CDS             48656..50038
                     /gene="hemN"
                     /locus_tag="PKN51_00905"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNT83684.1"
                     /translation="MQAITPELLTKFDVPGPRYTSYPTADRFVEAFGEPDYLRALDQR
                     RTGPVAGARPLSLYVHIPFCESLCYYCACNKIITKHHDRSAPYLRYLSKEVDLHVQHL
                     GAGQSVSQLHLGGGTPTFFSDDELGELMSMLRRNFNLVAGGEYSIEVDPRTVDATRLQ
                     TLARLGFNRLSFGVQDFDPLVQKAVHRIQPAEQVFALVDAARRIGFESINVDLIYGLP
                     RQTPESFDRTLAQVAQLRPDRIALYGYAHLPERFKPQRRIVAVDLPGAANKVAMLARA
                     LAAFDAAGYDYIGMDHFALPDDALAVAKRQGRLHRNFQGYSTQPDCDLIGLGVSAIGK
                     IGATYSQNAKALEDYQDLLDQGHLPVVRGLAVSRDDLLRRAVIMALMCQGEVLYESIE
                     LAHLIRFQDYFASELETLRGMQAQGLVRLTDTGIEVTPMGWFFVRGVAMVFDKYLQAD
                     RTRARFSKII"
     gene            50038..50721
                     /locus_tag="PKN51_00910"
     CDS             50038..50721
                     /locus_tag="PKN51_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT83685.1"
                     /translation="MQSSLAVTALVMGLAGGPHCIAMCGAACAGIGQAAAPRSTQALA
                     LFQLGRLTGYAALGALAAATMQGLGWLTVQSAALRPVWTMVHVAAVLLGLSLLAFARQ
                     PVWLEAGARRVWARVRTATQSLGLVAPLGLGVAWALLPCGLLYSALMVAALAGSVGGG
                     AAVMALFALGSGLWLGLGPWLLLRLGRSGRGAWSMRVAGLALAATSAWGLWMGLVHDQ
                     APWCVVPTP"
     gene            complement(50809..51633)
                     /locus_tag="PKN51_00915"
     CDS             complement(50809..51633)
                     /locus_tag="PKN51_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT83686.1"
                     /translation="MPIEPLLFIELAALGLATGFLAGLLGIGGGMIMVPFISTMLVGR
                     AVAPGLAVKMAIATSMATIIFTSMSSVRAHHKKGAVRWDIVKRLAPGIVLGGLVASLG
                     VFALLKGAALYFVFAGFVAFSAVQMYLDKKPAASRQLPGTTGQLAAGSVIGFLSGLVG
                     AGGGFVSVPFMTWCNVAIHNAVATSAALGFPIALSNVLGYIVAGQGLQGLPAYSFGYL
                     WLPALVVIASCSVLMAPVGARAAHALPVKKLKRAFAGVLFVLALYMLNKGIGALTG"
     gene            complement(51965..52300)
                     /locus_tag="PKN51_00920"
     CDS             complement(51965..52300)
                     /locus_tag="PKN51_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNT83687.1"
                     /translation="MKQVEVRIMGQAYLLAAPEGGEQRLQQAVQRVDAAMCKIRDAGR
                     IKARDRIAVLAALNLAFELTDAPRPADAATTAPTDTPAGADEPASQAALGALLARLDQ
                     ALGNDGQLL"
     gene            complement(52297..52560)
                     /locus_tag="PKN51_00925"
     CDS             complement(52297..52560)
                     /locus_tag="PKN51_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HNT83688.1"
                     /translation="MTTPPLIEQLSERVERLLVRHEELQRTNDLLAQQVQALTHERDN
                     LRSRLMAARARIDSLIDRLPATAGVLSGEATPDTPPAGKEATR"
     regulatory      52620..52888
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            52983..54815
                     /locus_tag="PKN51_00930"
     CDS             52983..54815
                     /locus_tag="PKN51_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT83689.1"
                     /translation="MSLRLQSFRPAALPLALACAFCAHAAQDTTVAQLAAPSLPDTVV
                     TATRVAQPLTDVLADVTVLGDEQIRASGAVSIGDLLARQPGLELARNGGLGSATSVYL
                     RGAETRFTAVYIDGIRIDSQATGGAPWEAIALGQVERIEIVRGPAAAVYGSDAIAGVV
                     QIFTRKGEGPFAPYVGVGLGSHRSGKLEAGFSGRNGALDYALGAARELSRGYNSMPAG
                     NPDRDGYRQSSASGRLGWNINDAHRLELSGTHTDIDAQYDGFLPGLDDHGLNRLGTLG
                     AAWSAKWSKRYSTRLSVSESDQRYETVPSPYLTKTRLRNYLLQNEWREGAHLITATLE
                     RREDHLVNAPIAQGRHQNAIALGYGFSGGPHTLQVNLRHDRDSEFGGQTTGGVAYGYG
                     FAPGWRATVAAGTAFRVPTLYQRFSEYGQPALQPEKGRNVELGLHWAQGDSRFGVSVW
                     RNRVSNLIVFGAPGPCAGAFGCYENAGRARLQGVTISGAHRLGGVNLSAALDLQQPRN
                     TLTDKLLPRRAQRLLKLSADIRVGGWTLGAEWQASSHRWDNAANTRRLAGYGIVNLYA
                     STTLARDWQVLARLDNLGDRDYQLAHGYATPGRSLFVGLRWTPR"
     gene            54839..55420
                     /locus_tag="PKN51_00935"
     CDS             54839..55420
                     /locus_tag="PKN51_00935"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNT83690.1"
                     /translation="MDVARSELILGGQKSGKSRRAESLAAAWLAAAPTHRAVLIATAG
                     AHDAEMRARIARHQRERAARVPGLVTHEAPHELAAAIAQAGGAETLRIVDCLTLWLTQ
                     RLMPLPGHESHGPAALDGQAPAAIIIEALRSCPGPIVLVGNEIGLGVIPLGAEVRAFV
                     DALGQLNQAVAARCQRVTLMAAGLPLTLKDAAP"
     gene            55417..56283
                     /locus_tag="PKN51_00940"
     CDS             55417..56283
                     /locus_tag="PKN51_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNT83691.1"
                     /translation="MSRWLLALLAWVWVGLVSAQELRDDAGQPLRLDAAPRRIVSLLP
                     SLTETVCALGACERLVGVDRYSNWPASVQTLPQVGGGLDPNIEAVVALKPDLVLIGTS
                     SRAALRLRALGLNVLALEPRTRAELRRVTQRLGTALRLDGADALARRIDDGVAAAAAA
                     LPVAARGQRVYYEITPAPHAAGQGSFIHELLLALGLVNVIDDPALGPFPRLNPELVVR
                     ADPDLIMVSQGGAADMARRPGWAGLRALREGRVCEFNAAQRDILVRPGPRMPEAARLM
                     ADCAARRPLQNR"
     gene            56319..57422
                     /locus_tag="PKN51_00945"
     CDS             56319..57422
                     /locus_tag="PKN51_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNT83692.1"
                     /translation="MNLHTRAMDAPAHAATAALPPQGGARVRWLALALLALALLGLGL
                     GASVGSTGFDSLLRAWHDAVAWQIVWDIRLPRTLGAWLAGALLGLAGAVAQGLFRNPL
                     ADPFLLGSASGAALAVALTFAALGAGGLVAGGHGVALLQAASPWLARLGFTGAAFVGA
                     LGGVLLALVLAQGVQQTLRLLLAGVVVGYVLGAVRELVQLAQPDVLQAMQGFSLGVTG
                     FVGWGACAVMAAVLAPLLPLAWALARGLDALSLGEATARSLGLPLGLLRALLIGVLAL
                     ATGTAVAQTGLIGFVGLVAPHLARALARCTHGPLLLLASLMGGLLLTAADIAARLLIA
                     PQELPVGVLTAVLGGLYLLWLMHRRGRAGGALQ"
     gene            57503..58228
                     /locus_tag="PKN51_00950"
     CDS             57503..58228
                     /locus_tag="PKN51_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT83693.1"
                     /translation="MALELPQGRWCAVVGPNGAGKSTLLRVLAGLLPCQGQISLWGRP
                     LHDWPARARARQLAWLGQQEGGGEDLLAWDIAMLGRLPHRPWLADASAHDQAIVADAL
                     RSMQVWALRGRRLGELSGGERQRVLLARLLAVQAPLLLMDEPLAHLDPPHQADWLAVV
                     RAQVAAGGTVVSVLHELNMALAADDIVVLRAGRLLHHGPAACPHTHAALRAAFDGRIS
                     IRQLERQWVALPQPDRSEPDHAD"
     gene            58218..58799
                     /gene="cobO"
                     /locus_tag="PKN51_00955"
     CDS             58218..58799
                     /gene="cobO"
                     /locus_tag="PKN51_00955"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNT83694.1"
                     /translation="MQIESPPTEQRYERPAGERRGLVIVHTGDGKGKSTAAFGLALRA
                     FGRQHVHGKQVVIYQFMKVPTARFGEHRAFEQLGLPVHGLGDGFSWKSQDLEHSAQLA
                     REGWQKARAAILDGQHFLVVLDEITYPLIYGWLPLGDVLHTLRQRPREVHVCLTGRRC
                     PPELIELADTVTEMTLVKHAFKAGIPAQRGIED"
     gene            58876..59412
                     /locus_tag="PKN51_00960"
     CDS             58876..59412
                     /locus_tag="PKN51_00960"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNT83695.1"
                     /translation="MPLTSHTYATPLGQMLALLSERGLCLLEFSQHTRGLGREIAQVE
                     AARGGPAQAGAHALGEQLGRELAEYFAGRRRHFGIPLDLVGTPFQQSVWQALLQIPYG
                     QTRSYAQQAQSIGRPTATRAVAAANGSNKIALIVPCHRVIGSDGSLTGYGGGMPRKQW
                     LLALERSGVPPGGGMDLT"
     gene            59731..>60010
                     /locus_tag="PKN51_00965"
     CDS             59731..>60010
                     /locus_tag="PKN51_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83696.1"
                     /translation="MNARNFIRRGITALAMVLGAASQAQAANVAFWSDYYSAASFTAL
                     NATHTVTQVTVANIDAGALSAYDVLVLGHLNPSIPPTATTCSQIQSFLA"
BASE COUNT         9343 a        20128 c        20727 g         9812 t
ORIGIN      
        1 tgcagtaccc cgacggctac atcaagatca aggaccgcag caaggacatc atcatttcgg
       61 gcggcgagaa catctcttcc atcgaggtcg aagacgtgct gtaccgccac cccgccgtgc
      121 tggccgcggc ggtggtcgcc aagcccgacc cgaagtgggg cgaaacgcct tgcgcctttc
      181 ttgagctcaa acccggtgcc gaggtcagcg aagccgacat cgtggcgcac tgcaagcagc
      241 acctggccgg cttcaaagtg ccacgcgcca tcgtgttcgg tgagctgccc aagaccagca
      301 ccggcaagat ccagaagttc gagctgcgca aacaggcggg ttcggccgcg gcgatcgatg
      361 tctgaatttg ctatcaggaa tatagctgtt ggcgcagttt gaacaagcgc tgagcggcga
      421 ttcgatgtct gatcgccaaa agggggtgtg gggcacccgc cggttcagtc ctgtggcgcc
      481 ggtgcctgcg gccgcagtgc ctccggcacg tcggtgcggc cggcgacgcg gtggcgaaac
      541 agcgccgcgc ggctcagcag cacggtggtg atcggcaccg tgatggacaa caggatgatg
      601 atgatccaca cgtgcagttg cggcccctgc tgctggcccg aaaagaacag gatcgaagcc
      661 agggtcacgc accaggagga gcccacgtac accagggccg gtgggtgcat gcgctggtag
      721 aagctggaca gccgcaccag gccccaggcg gccgccagag cgaacacgcc gctggccagc
      781 agcaggccgg cgatcagcca ctgcagccat tgcggcaggg cgtcggcgct cattcgatca
      841 cctcgccgcg cagcaggaac ttggccatcg ccgccgaacc gacgaaaccg aacagggcga
      901 tcagcagggc ggcctcgaaa tacaggtcgc tggcgtggcg gatgcccagc accagcatga
      961 tcagcatggc gatgacgtag atcatgtcca gcgccagcac gcggtcctgt gccgtcgggc
     1021 cgcgcagcac gcgccacagg gcgatcagca tggcgacggc gtaggcaccc agcgtcaggg
     1081 ccagcgcgcc ggagagcagg gggctcatga ctcgaagatc tccttcagcg gttgttcgta
     1141 gcgtcgtttg aagaaggcga cgaattcggc ctcatcgggc acatgccaca cgtgcagcag
     1201 catggcgctg cagtcgcgcg ccagttcgca ccagacggtg ccgggcacca cggtggtgat
     1261 catggtgagc gaggccagcg cggtagggtc gcgcagcgtc agcggaatca cgacgaagcg
     1321 gctgtggacg ggtcggctgc ggttgcgcac cagggtccag aacacgtcca ggctcgacac
     1381 caacacgtcg tgacccacgg tcaggatcag tcgcagcacc ttcagcgggt gccgtatccg
     1441 cgggcgcgcc gggcgcagcg gtgccagcag cgccggcgcc gccacgccga acagcagcgc
     1501 cagcggcagc tgtcccaggc tgaacggccg gttcagcacc agccacagca ggatcagcgc
     1561 cgccgtcagc agcggtgcgg gcagccagcg tctcatcggc gcagtcctcc ctggtcgctg
     1621 gcgtggggcg aaggctgcgg ccgcgccgcc atcacgctgc cgatgtagtc gcgtgggtcg
     1681 tgcaggtggc gcgcggtggc gttggcgtag cgcagcacgg cgtcggcctg ccacaccagc
     1741 acgccgatgg gcagcagcag cagggcgatc ggcagggttt cgatgatgcg caggcgcggc
     1801 ggtggccggc cggcgggcgc ccagaagcag cgtatgccgg cgcgcgacat ggcgatgatg
     1861 gtcagcaacc cgcccgtcac cagcagcgcc agcagcagcc aggcggccgg cgaaacgggt
     1921 gcctgcacgc cgaagccggc cgggttgagc agcgccgaca gcatcaccac cttgccgacg
     1981 aagccggcca tgggcggcag gccggcaatg gtgagcgcgc agacgatgaa ggccagcccc
     2041 aggaaggcca tggcggccgg aatggcgcgg ccgatcagcg cctcttcgtc gtcgtcgagg
     2101 ttgacgccgt gcgtgggcac cagatcgacc gggaaggcaa acggttcgtc gtccgggtcg
     2161 gactcgccca cgtacaccgg ctgctcttcg acgtcgcgtg cgcggtcgat cagctccacc
     2221 agcagaaaga aggcgcccac cgccagcgtg gagcccagcg cgtagtacag cgcgccgccc
     2281 gtcaccgccg gccgaccgaa gccgattgcg gccagcagcg tgcccgacga cacgatggca
     2341 cagaacgccg ccatgcgccc cagctgctgc accgcgatca cgccgatcac gccgaagccc
     2401 agggtgagca tgcccagccc gatcagcacc gggccgccga acaacgcgga gatgccggcg
     2461 tcctgcggga acagcagcgt ccacaggcgc aggatggcgt acacgcccag cttggtcatg
     2521 accgcaaaca gcgccgccac cggcgccgcc gcgctggcat aggcggccgg cagccaggca
     2581 ttgaacggcc aggcggcggc cttgaccagg aaggcgatcg ccaggatggc ggcgccggcg
     2641 tgcagcagac cgcgatcgtc ctcggcgatg gtcggcacga tgatggccat gtcggccatg
     2701 ttgagcgtgc cggtgatgcc gtacagcatg gccaccccga tcaggaacag gctggacgcc
     2761 atcaggttga tggcgatgta gtgcaggccg gcgcgcacgc gcggccaacc ggacccatgc
     2821 agcagcaggc cgtaggaggc cgtcagcatg atctcgaaga acacgaacag gttgaacagg
     2881 tcggccgtca ggaaggcgcc ggccaggccc atcagctgca actggtacag cgggtgaaag
     2941 tgcaccccgg cccggtgcca gcgcgcgctg gcgtagagca cgccggccag cccgagcagg
     3001 ctgcccagca gcagcatcat ggccgacagc cggtcggcca ccagcacgat gccatagggc
     3061 acgggccagt tgccaggcag gtagacaccg atcgactgcg ccgcgccact gcgctgcacc
     3121 tgcagcacca gcgcggcagc caccagcacg ccggcggcgc tggccagcac gttgagcgcg
     3181 gccttcagcg gccggcgcgt gtcgcccagc agcagcatca gcgcggccgt cagcatgggc
     3241 aacaggatgg gcgccaccac caggtgcggc atcagggact cgatccacgc ggacaggttc
     3301 atcggggtgg ccctccggcg tcgtcgcgaa gcaagggcag ggccgtgttg tcgtcgacgt
     3361 tgtcgccgtc cacgtggtcg ttgtcggcca ggccgcgcgc ggccatcagc accaccagaa
     3421 acagggccga ggtggcaaag ccgatcacga tggcggtcag caccagcgcc tgcggcatcg
     3481 ggtcggtgta gtgctccagg ctgggagtca ggccgggctg cacgatgggg ggcttgtcct
     3541 cgaacaggct gcccatggca aagatgaaca ggttgaccgc gtacgacagc agcgacaacc
     3601 ccatcagcac ctggaaggtg cgcggacgca gcaccagcca cacaccgcaa ccgcccagca
     3661 cgccgatggc cagggccagg atcacttcca tctcagccga cctccggcag gcggtgggcg
     3721 ggagcgtcgg tggccatcgt ctccggtgcc tggcgatacg cgcgcaccga ctggtgcgcc
     3781 agcgccgtca ggatcagcag cgtggcgccc accaccacgg cgaatacccc caggtcgaaa
     3841 aacagcgcgc tgggcaggtg gacatcgccc agcagcggca ggtgcaggtg cgcggtgtgc
     3901 gtggtcagca gcggaaagcc cagtggcagc gagcccagac cggtgagcgc ggccagcagc
     3961 aggccgaggg cgatccagcg gatcaccttc agttgcagat gcgcttccac ccacagcgtg
     4021 ccggccacga tgtattgggc gatgaaggcg atcgccacca ccagaccggc gacgaagcca
     4081 cccccgggct ggttgtgccc gcgcatgaac aggtgcatgg cgatcaccgc cgccaccggc
     4141 agcagcaggc gcaccagcac cgccggcacc agcatgtagc ccagcgccgt gtcctgtgcc
     4201 gtgcgcggat tgatcaggtc gctcaccaga tcggaggcga tggcgcgttg ctgcggcggc
     4261 agcgtggcca tctcgcgcgg cggacgaaag cgccgcagca gggcgtacac ggtgagcgcc
     4321 acgatgccca gcacggtgat ttcgcccagg gtgtcgaagg cacggaagtc caccagcatc
     4381 acgttgacca cgttgttgcc gccgccttcg ggcagcgcgc ggtcgatgaa gaagggcgcg
     4441 atgctctgcg gcgccgggcg cgtcatcatg gcgtaggcca gcgccgccat gccggtgccg
     4501 gcaaccccgg ccaccagcag gtcgcgcagg cgccgtgcgc gcacccgcgg gtgcaggcgc
     4561 tggcctggct gcggcatgcg cttgggcagc cagcgcagac ccagcaaaaa caggatggtg
     4621 gtgaccacct ccaccgtcag ctgggtcagc gccaggtcgg gcgccgacag ccagacgaag
     4681 ctcaggctca ccaccaggcc cactgcgccc agcatgatca gtgccgccag ccggtggaac
     4741 ttggcctggt gagcggtgcc cagggcggcc agcccaccga gcacccacag cagggcaaac
     4801 gccgcgctgc cgggcacgcg ctcgcgttcg ccccagctga tgccggtcgg ccacagcgcc
     4861 gccgcgccca gcacgatggc cgccagcacc accaccagca actgtggctg caggcgtggc
     4921 gtgctcagac gcctgagcag gccgcggctg gcgcggtcca gacccagcag caggcgctgg
     4981 aacagccgct gcccgtccag ccggcgcggc agcgccagca gccgcaggtc gcccgcggcc
     5041 aggcgctgcc ggccccacag gaacatggcc acgccgccga ccagggcgat caggctcatc
     5101 agcagcggct tgttgatccc atgccagatc gccaggctgt aggccggcat cgccccgccc
     5161 accaccgggc gggccgccag ctccagcgcc gcgccgatgg cccattgcgg cagcacgccc
     5221 accagcaggc acaccagcac cagcacctcg accggtgcgc gcatccagcg cgacggttcg
     5281 tggggcgtca gcggcaggtc cctggccggc gggccgagga acacgccgac cgcgaaacgc
     5341 agcgaataca ccacggcaaa aacgccggcc agcgtggcga cgacgggcaa gccgatgtcg
     5401 agcgtcgggt tgcggctcag gtgcacggtt tcggcaaaga acatttcctt ggacagaaag
     5461 ccgttcaaga gcggcacgcc ggccatggcg gcgctggcca cgaacgccag cgtgccggtg
     5521 atcggcatgg cctgccacag gccggacagg cgccgcagat cgcgggtgcc ggtttcgtgg
     5581 tcgatgatgc cgacggccat gaacagcgag gccttgaagg tggcgtggtt catgatgtgg
     5641 aacaccgccg ccaccgccgc cagctcgctg cccaggccca gcagcagggt gatcaggccc
     5701 aggtggctga tggtcgagta ggccagcagc cccttgaggt cgttctggaa catcgccagc
     5761 agggcgccca gcagcagcgt caacaggccg gcgctggcga cgatccagaa ccaggcttcg
     5821 ctgcccgcca gcgccggcca cagccgggcc agcaggaaca ccccggcctt gaccatggtg
     5881 gccgaatgca ggtaggccga caccggcgtg ggcgccgcca tggcgtgcgg cagccagaag
     5941 tggaacggaa actgtgcgct cttggtcagc gcccccagca gcaccagcag catcatgggg
     6001 acgtacaggc ggtgcgcccg gatctgttcg cccgcaccca ggatgtcgtc cagccggtag
     6061 ctgcccgcga tgtgacccag cagcagcaca ccggccagca gcgccaggcc gccggcgccg
     6121 gtcacggtga gcgccatgcg tgcgccgcgg cgggcgtcct tgcggtggtg ccagtagccg
     6181 atcagcagga aggaaaacag gctggtcagc tcccagaaga acgccagttg caccacattg
     6241 cccgacagca ccacgcccag catggcgccc atgaaggcga ggaagaacga aaaaaagcgc
     6301 ggcaccgggt cttcgcggct catgtagtag cgcgcgtaca gtgccaccag ggtgccgatg
     6361 ccggtcacca gcagggcgaa catccaggca aagccgtcca tgcgcagcga gaagtcgatg
     6421 ccggccgagg gcagccagcg cagctcctgg cgcagtacgg cgccgccctg cagcgccggg
     6481 tagcgcgtgg ccacccacac cagcgccgcg gcgcccacca gggccgccca gcccgccgcc
     6541 gcgttgcgcg catgcgtggg cagcagcgcc gccaccaggc tgccggcgaa ggggagcaac
     6601 aacaggtaga tgaggtccat cgggtggctg gggcgatcgg cggggcgggc gcggcggcag
     6661 gtgttcccgg gtcgccgctg cagcaccgaa tataccagtg ccattcatgg cttgggcgct
     6721 tgtgggacaa gcgccagaag ctatcaaaat aggagttttg tggaagctgt cgtgcggcct
     6781 cacaatgccg gcatgcggat gctgctggtg gaagacgacg ccatgatcgg cgaggtggtg
     6841 ctggacgcgc tgcgcgccga gcaccacgcg gtggactggg tgcgtgacgg cgccatggcg
     6901 ctgacggcgc tggccaccgc cccctacgat ctgctgctgc tcgacctggg cctgccgcgc
     6961 caggacggtc tgagcgtgct gcggcagctg cgcgccgacc accagcggct gccggtgctg
     7021 atcgccaccg cgcgcgacgc cgtggccgac cgcgtggccg ggctggacgc cggcgccgac
     7081 gactacatcg tcaagcccta cgacctggat gaactgctgg cgcgcgtgcg tgcgctgctg
     7141 cgccgcgccg acgggcgcgc cgagccggcc tacgaatggg gcgaggtgcg catcgtcccc
     7201 agcacgcgcg aagtcacgct gcgcggccag ccggtgcagc tgtcggcgcg cgaatgggcg
     7261 gtgctggaac ccctgctggc acgcccgggc cgggtgctgt cgcgccagca gctggaagac
     7321 aagctctacg cctggcgcga cgaggtcagc agcaacgcgg tggaggtcta catccacggc
     7381 gtgcgcaaga agctgggcgc cgggctgatc gagaacgtgc gcggtctggg ctacctggtg
     7441 ccggtggcgc cgcctgagtc gggttcatga gtgctcctgg aatggaagct gccagcgtaa
     7501 gtccatcaag cgctgagagc caaaaagact tgaagacgcc ggccccttcg ctgcgcgccc
     7561 gcctgacggc gctgctgctg ctggcggtgc tgctgacggc ggcgctgcag gcggccatca
     7621 cctggcgcac ggcgctggcc gagaccgagg cggtgttcga tgcgcagatg gagcgcatcg
     7681 cgctgtcgct caccggcggc ctggccgcca gcgtgctggg cgacgacgcg ctggcggcca
     7741 cgcgcggcgc cgaggagctg atcatccagg tctggcgcgc cgacggcatc atgctgtacc
     7801 gctcgccctc ggcgcggctg ctgccgccgc agacggtgat cggcttttcc cacctgcgcg
     7861 ccggcgacga ggactggcgc gtgtacgcgc tggagacacc cagccaggtg atccagatcg
     7921 cgcaggccga gcgcgcgcgc cggcgcatcg ccggccagct ggcgctgcgc gcggtgctgc
     7981 cggtggcgct gctggcgccg ctgctgatgc tgatcgtgtg gtgggtggtg gggcgcgcgc
     8041 tggcgccgat cgagcgcctg cgccgccagt tggccgcgcg tggtgccgcc gacctggcac
     8101 cgttgccgac tggcggtctg ccggccgagg tgttgccgct ggtgaccggg atgaacgggc
     8161 tgctggcgcg cctggcggcc gcctggcagc aactggccga cttcaccgcc gacgccgcgc
     8221 acgagctgcg ctcaccgctg gccgcactgc gcctgcaggt gcagagcctg cagcgcgccg
     8281 acaccgagca ggcgcgcgcc gtggccaccg ggcggctgct ggccggggtc gatcgcgcca
     8341 cgcgcctggt cgagcagttg ctggcgctgg cacgccacga tggcgatgcg gacgcggtgc
     8401 gcgagccggt cgatctgccc gtgctggcgc gccaggcgct gcaggaggcc gacgccgagg
     8461 cgcaggcgcg cggcatcgcc ctgcggctgg aaggtgatgc gtcgctgtgg ctggacgccc
     8521 ggccgcaggc gctggcggtg ctgctgcgca acctgatcga caacgccttg cgccacacgc
     8581 cggcgggcgg gcaggtgtgc gtggcggtgc agcgtcggcc cgacggcgtg gccgagctgg
     8641 cggtggaaga cagcggcccc ggcatcccag cggccgagcg ccagcgcgtg ctcgaccgct
     8701 tctaccgtgt gccgggcgcc gccggccacg gcagcggcct gggcctggcg atcgtgcagg
     8761 cggtggcacg ccggcatggc gcgcaggtgg tggtgcaagc ctcgccgcgc ctgggcgggg
     8821 cgcggctggc ggtgatctgg ccggccgccg ccgggcctga ggactgatgg tgttttaggc
     8881 atccagagca cgccagacaa gcgccaaaag ctattgaaat catagtattc agcagccctg
     8941 gcgtggtctt aagcctggcc taattcccat ccatcacagt gccgccacgg ctccgctgac
     9001 ggagcgtctt gctgaaagga actgtgatga accgtttgac cttgaaatcc tcacgtctgt
     9061 ttgccgccct gctggcggcg ggggtgatcg gcggcgccgg cgtggcggcg gtgcaggagc
     9121 tgacgcccgc ccaggcgcag ccgagcgcgg ccgtgcaggc ggtggcgccg gcggccgtgg
     9181 cgacacccga cttcgccgcc attgccgcgc gctacggcgg cgccgtcgtc aacatcagca
     9241 cccgcggcgt gcagccggtc gccgacgagg gtgccgacag cggcccgggc gctcggatcg
     9301 acccggggct gcaggacttt ctgcgccgct tcggcttgcc gcccggcgcg ttccagttcg
     9361 ccccgcccgg ccccggcggg cccggcgcgg tgccgatgca tggtcagggc tcgggcttca
     9421 tcgtcagccc cgacggcctg atcctgacca acgcccacgt ggtgcgcaac gccaggaccg
     9481 tcaacgtcaa gctgaccgac cggcgcgagt tccaggccaa ggtgctgggg cgcgacgaca
     9541 agaccgacgt ggcggtgctg aagatcgatg ccaccgggct gcccaccgtg ccgctgggca
     9601 gcagccaggc gctgcgcccc ggcgactggg tgctggcgat cggctcgccg ttcggcttcg
     9661 agaacaccgt gacggtgggc gtggtcagtg ccaagggccg ctcgctgccg gacgacagcg
     9721 cggtgccctt catccagacc gacgtggcgg tcaaccccgg caactcgggc gggccgctgt
     9781 tcaatgcgcg cggcgaggtg gtgggcatca actcgcagat ctacagccgc tcgggcggct
     9841 accagggggt gtcgttcgcc atcccgatcg agctggccag gcacgtcgag cagcaactcg
     9901 tcgcgcacgg caaggtcgag cacgcgcgcc tgggcgtgac catccagtcg gtcaaccagg
     9961 ccctggccga ctcgttcaag ctgcccaaac ccgagggcgc gctggtggcg caggtcgagc
    10021 ctggcagcgc ggccgagcgc gcaggcctga aggcgggcga cgtgatcctg cgcgccaatg
    10081 gccaggcgat cgtcgattcg ggcgacctgc ccgcccgcat cgcgctggcc cggcccggcg
    10141 acaagctggc gctggaggtc tggcgccagg gccggcccga gcagctgagc gccacgctgc
    10201 agggcgcgcg gcctgccgac accgcgctgg cgcagggcag cgcggccagc cacggcaagc
    10261 tggggctgtc actgcggccg ctgcaagccg acgagaagcg ccagaccagg ctggacgagg
    10321 gtctggtggt cgagcaggcc agcggcgccg ccgcgctggc cggggtgcag ccgggcgacg
    10381 tcgtgctgtc gatcaacggc cagccggcca gcagcgtcga gaagctgcgc gccgtgctgg
    10441 ccaaggccga caagtcggtg gcgctgctga tcgagcgtga cggtcagcaa ctgttcgtgc
    10501 cggtgccgct ggcttgaaac ctgcccggct tctgaatgac ccacgacctc gcggcgcttc
    10561 gatcaccgca ggggaagggg tcatcaatat caaatcgggc tctggcgctt gctgggcaag
    10621 cgccggtagc tatcgttggt gaagtttccg ggagggtgca cgatcggtgc cacacggtgc
    10681 cccggccagg tcccgatcgg gtggggctgg ccgcgctgct gcctgcgggc acgcgttggc
    10741 ggcggcgtcg cgccttgccg ggcgcgccga tcgcgttgcg ggcaggtgcg ccctgcggcc
    10801 gcttgcaggc tcagcccagc aactgcgcgt cgcggcgcaa cacggcatcg gcggtcacgt
    10861 caccgcctgt gcgcaggatg cggaacgcgg gctgcggcgt ggccgccaag ctcagccgca
    10921 gcacataggc ctcaccctgc gtgccgggca gcgcctggta gagcagggcg tagcgaccgt
    10981 tcggcacgac gacgcggtga cgctcaaaaa gcgccccgat gtgcagcggc tgggtgacct
    11041 ccagcggcat ctgcaccgcc cacagcgcct gtgcgctgac cgtggcctcg ggcaccagtt
    11101 cgacctggat gcggcactcg ccgtcgtggt ccggcacgcc cagagtcagc agcttttcgc
    11161 tccaggcaaa accctgcgcc acatgctcgt cggtccacag caggccggcg cccggctcgc
    11221 cttcgccgtg gaccaccacc tggccatgat cgatgctcac cacgaaatcc gccaacatcg
    11281 ttgcggtgtc ctggtcggtg tggggtaggg ggcggtgtgg gggcatcggt gttcggatgc
    11341 cgatgtcaga taaaaaatgg ctccggcgct tgctgggcaa gcgccagcag ctatgcattc
    11401 aggagttatc agggccgcct tcagcgcgtg taacccaggc aggcgccggc gttggggcgg
    11461 ccgcggcatt cgcggcgcag gcgcgcgtcc tcgttgcgct ggcgctcggc cggcgattcg
    11521 ccgctggcat aggtggcggt gggcgaaggc ttcaggcgta cgccgcgcga gccggcgccg
    11581 ctgcgcggtg tcttgcccgc gccttctttc ttggcctggg ccagcgtggg cgaggcgatc
    11641 agcgtggcga gcagggcgat ggcggccagg cggcgcgtgg gcaggggcat ggtggtcttt
    11701 ccgctggtgc agggccgcgc tcgcacggga tatgcgatgc tcgcgccctg ccgaattaaa
    11761 tgagtccgtc accgatgcgt caagccgtcc tgaacctcga agaatccaag atccgcgaag
    11821 tcgccaacgc cggcatgggc cgcgccgacg tgctgccgtt ctggttcggc gagagcgacg
    11881 aggtcacgcc cgaggcggtg cgcgccgccg gcatcgcctc gctgcaggcg ggcgagacct
    11941 tctattccca caacctgggc ctgcccgagc tgcgcgaggc ggtggcggcc tacgccagcc
    12001 gcctgcacgg cgagctggcg ccgcagcggc tggccatcac ctcgggcggg gtgaacgcgc
    12061 tgatcgtcgc catgcaggcg ctgatcgacg ccggcgacga ggtgctggcc gtcacccccg
    12121 tgtggcccaa cctgacggcg cagccgcgca tcctgggtgc ccagctgcgt tgtgtggcgc
    12181 tgcaacccga cgccgaaggc gcctggcagc tcgacgtgcc ggcattgctg gccgccatca
    12241 cgccgcgcac gcggctgctg gtgctgaact cgcccaacaa cccgaccggc tggacgctga
    12301 cgcgggccga gcagcaggcg atcctggcgc attgccgcca gaccggcacc tggatcctgt
    12361 cggacgaggt ctacgagcgt ctgtacttcc gcgacgacac cgccaacggc gcggcgccga
    12421 gctttctcga catcgccgcg ccggacgacc ggctggtggt ggtgcagagc ttttccaagg
    12481 cctttctgat gaccggctgg cgcctgggct ggctggtgat gccgccggcc atgacgccgc
    12541 aggtcggcaa gctgctggag ttcaacacct cctgcgcgcc agtgttcgtg cagcgcgccg
    12601 ccctggtggc gctggcgcag accgaggcca tcacgccgcg catcgtggcg cacctgcggc
    12661 actgtcgcga caccctggtg ccgctgctgc aggccgtgcc cggggtgcag gtcggtcgcg
    12721 cgcccggcgg catgtacgcc tttctgcgcc ttgtcggcca gaacgattcg ttgcagctgg
    12781 ccaagcgcct ggtgcaggaa gccgggctgg ggctggcgcc gggcgtggcg tttgcgccgc
    12841 aggccgaggg ctggctgcgc tggtgctttg cctcgcgcga cgtggcgcgg ctgcggcagg
    12901 gcgtggatcg actgcaccac tggctgcgga ccgccgcgcc cgccccatga acccggggtc
    12961 gcgcccgcgc cggcgtgtgc cagcgctgtc cggtgcgtcg ttgtcgcgac ggctgcgtct
    13021 ggcgcagctc gacctgttcg aaaaggtgct ggaaaccggc tccattctgg ccgcttcgcg
    13081 cgagctggcc atcacccagc cggcggtcag caagtcgctg cgcgagctgg aacagcagct
    13141 cggcggggcc ctgttcgtgc gcggcaaaca cggcgtgaca ctgacggagt tgggccagct
    13201 cttcgcccgc cacgcgcgtg ccatgacctc cggtctgcaa gacatggccg acgaactcaa
    13261 cgcctggcac gggggcaccg ctggacgcgt ggtggtgggg gccatgctga gtgccacggt
    13321 ggcgctgctg ccggaggcga tttcgcgctt gcgcgacatg gcaccggacg tcctggtgac
    13381 gattcgggtg gggcccaatt cggtgttgtt tccggccctg gcgcgcggcg agatcgatct
    13441 catggtgggc tatctgccgg ccaatgcgtc ggccgccaca caggatgccg ctcccggcac
    13501 gcgcctggtg catgtgccgc tgtacgacga aggcatgagc gtggtggtcg ggcccgacca
    13561 cccgctggcg caccggaccg acgtgcgcgt ggccgaactg caccggttcg agtggatcgt
    13621 gccgacaccg gacgccgtgg cctatccggc cgtgcgcgcc ttgttcgagc gcgaagggct
    13681 gagtctgccg cgcagccgcg tcgaatcgat ctccatcctg accaacgtgg gtctgctcgc
    13741 gcgtcgcccg atggtggcgc tgatgccgca ctcggtggct acgcgcttgg ccagtgccgg
    13801 tctggtggtc gttctgccgc tgcccagcct ggggtcattc agcgcgctcg gctatacccg
    13861 gcgcgccgag cagggaatga acatggccct gcagcatctg atcgatgcct tgcatgcggt
    13921 cggcgacgcc ttgcagcggg gcgcaggttt ggatctgtcc tgagcggcgg gctcggcgaa
    13981 catctatatc cgagagttat agatgagtca cccgaactca tttgccagcc gcaggtggcc
    14041 tgcctagact gcgcgatatc agacgtcacg gggcaggtgt gacccggacg gcacgaggag
    14101 cgcgtcttgg gacgatcttt cgcactgccc gccacgctgg gctcgctgcg caactatgtg
    14161 gatggcgaat tcgtagaaac cggcaggacc ttcgacaacg tcagcccgat cgacggcagc
    14221 gtgctggcca aggtgcacga ggccgacgcc gccctggtcg accgcgccgt gcgcgccgcg
    14281 cgccgcgcgc tggatgaagg cccctggggc cgcaccaggg tggagcagcg cgccgaggcg
    14341 ctgcaccgcg tggccgacat cattgcccgg cgcttcgacg agtttctgca ggccgaggtg
    14401 gccgacaccg gacgcccggt ggagcaggcg cgcaagctcg acgtctaccg cggcatcgcc
    14461 aacttccgca gctttgccga cctgggcaag accgcgcacg gcgagttcta cgaaacccac
    14521 ctgcccgacg gcagcgagct gatcaactac accaagcgca aaccgctggg cgtggttgcc
    14581 agcatctcgc cctggaacct gcccatcctc tcgctcacct ggaaggcggc gccggcgctg
    14641 atctgcggca acaccatcgt gtgcaagccg tccgaggaaa cgccctcgtc ggcggtgctg
    14701 atcgccgagg ccttccacga ggctggcgtg ccgccgggcg tgttcaacgt ggtgcatggc
    14761 ttcggccccg gctcggccgg cgaggcgctg accacgcacc cgggcatcga tgccgtcacc
    14821 ttcaccggcg aatcgcgcac cggcgcggcc atcatgcagg ccgtggcgcc gggggtgaag
    14881 gaagtctcgt tcgagctggg tggcaagaac gccgccatcg tgtttgccga ctgcgatttc
    14941 gaacccacgg tggagggcat ggcgcgcgcg cttttcacca acaccggcca ggtgtgtctg
    15001 tgtcccgagc gggtgttcgt cgagcgatcg atcttcgagc gcttcgtcgc cgcgctcaag
    15061 gccaagaccg agtcgctcaa gatcggttgg cccgacgagc cggacgtggc catgggctcg
    15121 ctgatctcgc gcgagcaccg cgagaaggtg ctgtcctact tcaggctggc ggtggaagaa
    15181 ggcgccaccg tggtgaccgg cggcggcgtg ccgaagtttg gcgacgcgcg cgataacggg
    15241 gccttcgtgc aacccacgct gtggacaggc ctgcccgaca gcgcgcgctg cgtgcgcgag
    15301 gaggtgttcg gcccggtctg ccacctgagc cccttcgaca gcgaggacga ggtgctgcgc
    15361 cgcgccaacg acagcgacta cggcctggcc gccaccgtct ggaccagcaa cctgtcgcgc
    15421 gcacaccgcg tggcacccca gctgcacgtg ggcatcgtct gggtcaacac ctggttctcg
    15481 cgcgatctgc gcacgccctt tggcggcacc aggctgtcgg gcatcgggcg cgagggcggg
    15541 cgctggtcgc tcgacttcta ttccgaaacc accaacatct gcatcaaggt ctgatgcccg
    15601 tcgccagcct tgcccaagga gccgtcatgc cgatcaccct gcaacagtgc cagacctggg
    15661 accaggccga cgaactggcc gctttgagag acgaattcga cctgcccgca ggcaccatct
    15721 acctcgacgg caattcgctg ggcgccatgc cccgcaacgc gctggcgcgg gcgcaggaca
    15781 tcatcgcccg tcagtggggc gtcgatctga tcaacagctg gaacaaggcc ggctggtggc
    15841 agctgccgac ccggctcggt gaccagctgg cgccgctggt cggggcgggc gcgggcgaga
    15901 tcgtggtcac cgacaccacc tcgctcaacc tgtacaaggt gctggccaac gcggtgcgca
    15961 ttcagcagcg tcggcagccc gagcgcaaag tgattcttgc cgagcgtgac gcctttccga
    16021 ccgacctgta catggtgcag ggcctgctgg gcttcatgga ccgtggctac acgctggagc
    16081 tgatcgaccg cccagaagac ctgccctcgc ggctgggcga gcaaaccgcg gtggtgctgc
    16141 tttcgcacgt caactaccgc accggctacc agtgggacct gtccgccgcc agccagcagg
    16201 cccacgccgc cggcgcgctg atcgtctggg acctgtgcca ctcgatcggc gccgtgccga
    16261 tcgacctcga ggccgccgac gccgactttg ccgtcggctg cacctacaag tacctcaacg
    16321 gcggccccgg ctcgcccgcc atgctgtggg ccaagccgcg ccaccgggcc gatttctggc
    16381 agccgctgtc gggctggtgg ggccatgcca ggcccttcga catggcggtc gactacgcgc
    16441 cggatcgggg tatccgcggc ttcctgtgcg gtacccaacc catcgtctcg ctgggcctgg
    16501 tggcctgcgg cctggagatc ttcgccaaga ccgacatgca caagctgcgg cgcaagtcgc
    16561 tggcgctgac cgacctgttc atcgccctgg tcgagcagga gtgcgccggc cagggcctga
    16621 ccctggtcac cccgcgcgag cacgcgcagc gcggcagcca tgtcagtttc cgccaccccg
    16681 agggctacgc cgtggtgcag gcgctgatcg cgcgcggcgt gatcggcgac taccgcgagc
    16741 ccgaggtggc gcgctttggt gtgaccccgc tgtacctgcg ctttgtcgac atctgggatg
    16801 cggtgcagca cctcaaggca gtgctgaccc agcgcgaatg ggaccagcct cggtaccgcc
    16861 aacgtggcgc cgtgacgtga tcgcagcatc ctgaaggaaa cctccatgac cgccactttc
    16921 aaccgctcaa cccaggccag cgtggtggcc ggcaaagcca cgccgcgcgg gcgctttccg
    16981 cacatcaagc gggcgggcga ctttcttttt gtgtcgggca ccagctcgcg ccggccggac
    17041 aacagcttcg ctggcgtgca ggtcgacgcg ctgggcgtga ccaacctgga catccgggcg
    17101 cagacgcgcg ccgtgatcga gaacattcgc gacatcctgc tcagccaggg tgcgcagctg
    17161 tccgacctgg tggaagccca ggtgttcctg gtcaacatga acgatttcgg cggcttcaac
    17221 gaaacctggg cagagttttt cgactacgaa ggcccgacgc gcaccacggt ggcggtgcat
    17281 cagctgcccc acccgcatct gctgatcgaa atcaaggcca ccgcctacca cccccggccc
    17341 ggcacgggcc agccacgctg aaggagacga ccatgcagcc gaaatacggc ctccccaaga
    17401 acctgatgga ctgggtcggc gcccacagcg accgcctgcg gccaccggtg tgcaatgcgg
    17461 ccctgtttcc tgacggcaac tacatcatca acatggtggg tggtggcaac acgcgcaccg
    17521 acttccatga caacccgacg gaagaaatct tctatcagat ccggggcacc gcctacctca
    17581 acatctggga ccgtggcaag ttcgatcgca tcgacctgaa ggagggcgac gtctggctga
    17641 tggagccgca tctgcagcac tcgccgcagc gccccgaccc ccatggcctg tgctttctgg
    17701 tcgagctcaa gcgaccccag ggcgccatcg acacgctcaa ctggtactgc ccccgatgtg
    17761 cggctctggt ctggggagcc tcgatggagc tgaaagatct ggtggccgac ctgccggcca
    17821 cctaccagaa gttctacgcc ctgagcgacg ccgaacgcac ctgtccgcac tgcggcaccg
    17881 tgcaccctgg caaggactac gccgcgtggc acgccatccg tgaggcccac gcatgagcgg
    17941 cgccttgtcg atgatcgata tccatgcgca tttcctgccg cagatcggcc agcaggaagc
    18001 cgccgcggtg gagcgcgagc gggcgccctg gctgcgcatc gacgccggtg gcgaaaccgg
    18061 ccagatcatg gtgggcgagc gcgagttccg acccgtctat cgggcgttgt gggacgcgga
    18121 tttccgcgtc gccgaactgg acgcccaggg cattgcgctg cagctcgtgt gcgccacgcc
    18181 gatcatgttc ggctacgcct gggaggccgg gcgtgccgct gactgggcgg cgcagatgaa
    18241 cgacaaggcg ctgtcgttct gcgcccgtca cccgacacgg ctgaaggcgt tggcccaggt
    18301 gccgctgcag gacaccgcac gggcgtgcgc ggaagccagc cgggccatgg ccgccggttg
    18361 cgtgggtgtg cagatcggca accacgtggg cgacaaggac ctggaccacc ccgacctggt
    18421 cgacttcctg attcactgcg cggaccaccg cattccggtg cttgtccacc cctgggacat
    18481 gatgggcggg cccgggcgca tgaagcaatg gatgctgccc tggctggtgt cgatggccgc
    18541 cgagacacag ctgggcatga tgtcgctgat cctctccggc gcgctggagc gcattccgac
    18601 atccctgcgt atctgctttg cccacggtgg cggcagtttt gcctatctgc tgggccgcgc
    18661 cgacaacgcc tggcggcagc gcgacatcgt gcgcaaggat tgcccgcgcc cgccgtccga
    18721 gtacgcgcgc cgcctgcata ccgacgccat cgtgttcgac ccgcgggcct tgcacctgtt
    18781 gatcgacgtg atgggggtgg acaacgtcat gctcggctcg gactacccgt tcccgctggg
    18841 cgagcagcag gtcgggtcgc tggtgcgtgg ggtcgacttc ctgtcgggca gccagcgcgc
    18901 gcagatcctg agcgacaacg cgcgccgctt cttcgccctg cccgaggggt gagcggcaca
    18961 cgctatattt caaccgtcaa acaacaactg gagacaacag atgaaatcca ctgtgcgcaa
    19021 cgtgattgca gccgcggcac tgtccatgct ggcgagccac gccctggccg acgtgaagat
    19081 cgggttcatg gccaccatgt cgggcagcgc cgccgcgctg ggtcaggaca tgtacgacgg
    19141 cttcatgctg gcgctggagc agaacggcgg caagctcggc ggccagaaga tcgatctgat
    19201 caaggaagac gatcagctca aacccgatgt gggcgtgcag atcgtgcaga agttcatcga
    19261 gaaggacaag gtggatatcg ttgccggcgt gacgttttcc aacgtcatga tggccattgc
    19321 caaaccgctc gcggacgccg acatgaccta cgttggctcc aacgccggtc ccgcgccgct
    19381 ggcgggcaag cagtgcagca agggtttctt cttcgcctcc tggcagaacg atcagcaggc
    19441 ggaagtcatg ggcgccttcg ccaacgacaa gggctacaag aaggcctacc tgctggcgcc
    19501 caactaccag tcggggcgtg accagatcgc cggcttcaag cgccagtaca agggcagcgt
    19561 ggtgggcgag gtgtacacgc agctgaatca acccgattac tccgccgaac tggcgcagct
    19621 gcaggccgcc agcccggacg tggtgtacgt gttctatccc ggcggcatgg gcgtgaactt
    19681 catcaagcag taccggcagg ccggcctgct gggcaaggtc cccctggtgt ccgcctcgac
    19741 ggtggacggt accacgttgc cggccctcaa ggagatcgcg ctgggcgccg tcaccggcac
    19801 gttctgggga cctgacttca acaacgccac cagcaaggcc ttcgtcgccg cgttcgagaa
    19861 gaaatacaac cgcatcccct cgcagtacgc cgcacaggcg tacgactcgg cattgctgat
    19921 cgacagcgcg ctcaagaaga ccggcggcaa ggtggcggac aagcaggcgc tgcgcacggc
    19981 gctgcgggcg gccgactacg cctcgctgcg cgggcccttc aagttcgatg tcaacggatt
    20041 tccgatccag gatttccatg tcttcgaggt cgccaaggac ggtgctggcc gggtctcgct
    20101 caagaccatc gccacgcccc tcaaggcaca ccgtgacgcg tatgtgggcg aatgcaagct
    20161 ctgatgcctt caccgctcga aaaggctgac gccgatgcgg accgtccgca tgcggtgcat
    20221 gcgttgggtc gggctgaccg ttcgggccgg cgcacgcgcc tgggcatggc gggcgagcgg
    20281 tcgtcgccgg caggggcggt gccgcggaca ggtgacggcc cgatgcccgc aggccgatgc
    20341 acggcgctgc aacccggttg cgcggcaagg tgagcccgtg cctgtgatgt cgtcccgcgt
    20401 ggatcgccgg gtgccgcatg acacctgagc tgctgctgat acagacgctc aatggcctgc
    20461 agttcggggt gctgctgttt ctgatggcgg ccggcctgac gctggtgttc ggcatcatga
    20521 gcttcgtcaa cctggcgcac ggcgcgctct acatgttcgg cgcctacttc gccgccgccg
    20581 cctggaacgc cagcggatcc ttcctgctgg cggtgctggg gggctttggt ggagcgttcg
    20641 tgcttggcgc gctggtcgat cgcgtcggtc tgtttgccct ttacccgcgc gaccacctgg
    20701 accatgtgct ggccaccttc ggtctggtgc tgtttgccaa cgaactggcg cgcatgatct
    20761 ggggcgcctc gccggtgttc atggaactgc ccgaatcgct tgcggcttcc gtgcccttgc
    20821 tgggcatcaa ctacccggtg taccggatgc tgatcatcgt cgtcgggctg gccgtggcgg
    20881 cgtgtctgta tctgctgatc gagcgcacgc gcgtgggcat gctgatccgt gccggtgcca
    20941 gcaaccgcgc catggtgtcg gcactggggg tgaacatcgg ctggctcaac acattcatct
    21001 tcgcgctggg cgccggtctg gccggtctgg cgggcgccat ggtgggcccg atcatgtcgg
    21061 tgcagccggg catgggtgat ccgatcctga tcaccacgct ggtggtcatc gtgatcgggg
    21121 gcatcgggtc gatccgtggt gccttcctgg gcgcgctggt ggtcggcgtg gtcgataccg
    21181 tggggcgcac gctggtgccg gcgctgctgc gcggcgcgct ggcgccggaa atggccaaca
    21241 cgctggggcc ggcgctgtcg tcgctggccg tctatgtgct gatggcggtg gtgctggcgc
    21301 tcaagccgga tggcctgttt ccggtcagga agcgctgagc ccagcaacgg tcctcgatca
    21361 cttcccctca tcctgagagc cgcatgaaca ctgggattca ctgcttcgct ggcctgcacc
    21421 tgccgggtga gtgcgcggcc cgaccgtcag catggcgtct gccacgcctt gcgggcggcg
    21481 ccctgcggcg tcgccgcgtg ttgacggtcg cgccgatcat ggcgcgggcg gacgcgccca
    21541 gcggctggtt ccgggactga aggggcggaa catggtttcg tcttcggtgg gtgtgcacgc
    21601 tgcggatcgc ctggcaccga cgttgctggg gctttccaag gcgcgctggc gaaccgtcct
    21661 cacggtgttg ctggcggcct tcttcgtgct ggtgccgctg tacgcctggc tgaccggcga
    21721 cagctacgtc ctgatcctga tgggccgcat tctggtgttt gcgctggctg cggtgggact
    21781 gaatctggcg ttggggtacg gaggcatggt gagtttcggg catgccatgt acctcggcat
    21841 cggcgcatat gtggtggcca tcgcttcgca ccatggcctg gacaatgggc tggtgcatct
    21901 gctgctggtg gctgccctca cggtggcggt tgccatcccg gtggggctca ttgcgctgcg
    21961 cacccagggc attgcgttca tcatgatcac gctggcgttt gcgcagatgt tgttcttcgt
    22021 cgccgtaggt ctgaagcagt atggcggcga cgaggggttg agcatcgtcc atctgtctcg
    22081 cttcggcgcc ctgacgggga acaagaccgc cttgtacctg tcgatgctgc tcgcgctggg
    22141 cctggcgctg tggctgttgc gtcgcgtcgt ggcgtcgcgt tttggcctgg tgctgcgcgc
    22201 cacgatgatc aatgaaaggc gggtgcgcgc ggtcggcacg ccgccactgg gctatcgcct
    22261 ggcgggctat gtggcgtcgg ccctgctgtg cgccctggcg ggttatttcc tggccaacct
    22321 gacctcgttt gcgtcgccgg cctacatggc ctggacggtt tcgggcgagc tcatcgtgat
    22381 ggtgttgctg ggcggcatgg gcacgctggt ggggccggtc gtaggggcgc tggtgtttct
    22441 gctgctggag gaggggctca aggcgctcac cgaccactgg ctggtcatca tgggcccggt
    22501 catcgtcgtc atcgtgctgt ttctgaagaa cgggctgtgg ggcatcgtct ccgacgtgcc
    22561 ggcagccagg ggagggcatt gaagtgacgc agtcgcagcc cttgctgagc ctgcggggtc
    22621 tggtcaagcg ctacggtggc ttgctggtga ccgatcatgt cgacttggac gtgctgcccg
    22681 gcgaggtgca tgccatcatc ggccccaatg gcgctggcaa gaccaccctg atcggccaga
    22741 tcgtgggaga ggtggccagc gacgagggca gcatccggct gcaggggcag tccatcgacg
    22801 gactgagcgt gcccgagcgc gcccgcgcgg gccttgggcg ctcctatcag atcacctcgg
    22861 tcatcggcgc tttcaccgtg ctggaaaacg tgattcttgc ggtgcagggc tgcctgggtc
    22921 atgccttcca tttctggcga ccggccagcg aaacggctga actggtggag cgtgccgatg
    22981 aggtgatcga gcgggtcggg ctgagtgcgc agcgtgatgt ccatgcggcg gcgctcgcct
    23041 acggtcagca acgccagctc gaaatggcca tggctctggc catgcagccc agcgtgttgc
    23101 tgctggacga gcccatggcg ggcatggggg cggtcgaatc gtcgcaactg gtggctctgt
    23161 tgcagcgcct gaaggggcag tacggcattc tgctggtcga gcacgacatg ggggcggtgt
    23221 ttgccctggc cgatcgcatc agcgtgctgt ttcacggcaa ggtcatctgc tgcggtgacc
    23281 ctgcgaccgt caaggcgcac ccactggtgc gggaagccta tctgggcgac gaggagattc
    23341 cggcgtgaac ctgcttgagg tggaaggcct tgccgccggc tacggtcaga gccaggtgct
    23401 gttcgacatc ggcctggcgg tggcacccgg tcaatgcgtc accttgctgg ggcgcaacgg
    23461 catgggcaag accaccacca tcaagaccat catggggctt ctgcgcccat ggggtggcgt
    23521 ggtgcgctgg aaggggcgcg ccgtccaggg gcgccccccc catgcgatgg cgcggctcgg
    23581 cctgggcctg gtgccggaag gccggcaggt gtttcccaac ctcaccgtgc gcgagaacct
    23641 ggtggccacc gggcgcggcg gcggttcgtc ccatgcctgg acgctggcgc gggtgcacgc
    23701 gctgtttccc cgcctggccg aacgggcgtc caacttcggc aaccagctgt ccggcggcga
    23761 gcagcagatg ctggccattg gacgtgctct gatgaccaac cctgagttgc tcatcctgga
    23821 cgaggccacg gaagggttgt cgcccctggt gcggcgtgag atctggcttg cgctgcgaca
    23881 gatcaaggcc gaaggcatgg cgattctggt ggtcgacaag aacctggcgc cgttgctgga
    23941 actggctgag cgccaccaca tcgtcgagaa gggccaggtg gcctggagcg gcagctccga
    24001 ggaactgcgc gaccagcggt ccctgatgca gcgctacctg tcggcttgaa cagggcagcc
    24061 tttggtgcag caggtttgcc ttcggccgac aaaggcatta aactgctagg ttacccgggc
    24121 ccttggcggc ccgggccatc ttccgcccag tgctgcaacc cgcttcggtg tgagcgcagc
    24181 cgtgttgggc ggactttcgt ttccgcgcag gcgcgccgtt ttcggatggg gcctggtcag
    24241 cggtttccgt ggccgctgcc gctgggcggc gtgccctgtt tgccaccttg ctcgtggaga
    24301 tgtgtcatgc gcgttctgcc tcacaccgat tcccccaccc ccgtggttgc ctcaggcgcc
    24361 acgctgcgca gcgcgcatgc ggttgcgcgc atggcgcccg gtctgcatct gattggcgat
    24421 ctgtacggct gtcgtggcga cacccgactg atgaccgacg ccgccacgct ggaggccttt
    24481 tgcaagcagg ccgtggccga cgccggcctg accacggtgg gcagcctgtt tcacagcttt
    24541 ggtgaaggcg agggcgtgac cggggccgtg gtgctggccg agtcgcacct ggccctgcac
    24601 acctggcccg aagacaacta cgtcacgctc gacgtgtacg tgtgcagcta caccaacgac
    24661 aactctgcca aggccgagcg cctgttcgac gccttgatgc aggcctttca gccggccgat
    24721 ccgcatctgc accgcgtcgt gcgcgcatga gcgcgccgat gagcgcgtcg atcggcaccg
    24781 gcggccaatt cgccgtcgag cagttgtcgc ccgacttcgg cttctatctg cgtaccaccg
    24841 ggttgctggc cgagcgccgc tcgtccatgc agcacatcga aatcgtggac acgccgctgt
    24901 tcggccgcgc catgcgcatc gacggttgct tcatgacctc ggagcgcgac gagttcttct
    24961 atcacgagcc gatggtgcac ctgcccgcca tcgcccatgg cgatccgcgc caggcgctgg
    25021 tcgtgggcgg gggcgacggt ggcgcggcgc accatctgct gcgctacccg aacatggcgc
    25081 gggtggtgct ggccgagctg gaccgtgacg tgatcgacat ggcgcgcgca tggctgccgg
    25141 ccgtgcatcg cggcgcttgg gatgaccctc gcctggagct gcgcctgggt gacggccgcg
    25201 ccttcatcga gtcgtgcgag agccggttcg accagatcgt gcttgatctg accgaccctt
    25261 tcggccccgc cgtggcgctg tacacctgcg agttctaccg tgcctgccgg cgtgcgctgc
    25321 ggcccggtgg ggtgctgtcg ctgcacgtgc agtcgccgat ccatcgcggc gcgaccatgg
    25381 cgcgcctgct ggcgtcgctg cgcagcgtgt tcccggtggt gcgaccgttt ctgcagtacg
    25441 tgccactgta cgggacgttg tgggcgatgg cgatggcgtc cgaccgcgcc gacccgctgg
    25501 cgctgacggc ggccgaggtc gacgcccgtc tggcgcgcca cggcctgaac gatctgcagc
    25561 tctacagcgg cgacacgcac ctggcgctgc tcagcctgcc gccgttcgtg cggcgcctgc
    25621 tggccgagcc ggcgcgcccg gtggtggacg gcgacagcct ggacgatccg agcctggacc
    25681 ccggcgcaga gcgcacgctg cggctggtgc gcggctgagc cgcgcccgca gcggtgtcgg
    25741 tcgcgggcga aattctacaa tttgcgtaat tgcgcaaaca gtcgtagcat acgaccctcg
    25801 tcaacttgtg gagtccatgc gatgaagaag ctgctgatcg cggtggcgct ggggctggcc
    25861 ggctggtccg ccggggccgt ggagacgggc atttcagccc gcgaggcgta tgaaaaggtg
    25921 cagagcaatg ccccgggtgt gctgttcgtc gacgtgcgcg acccggtgga aatcatgttc
    25981 attggcttca ccgatgtggt gcacaccaac atcccgttca tgatggtgga ccgctcgaaa
    26041 tgggacgagc agcgcgggat attccggctc tacccgaacc ccgatttcgt ggcgcagatc
    26101 aaggccgagc tggcgcgccg cggcatgggc atggatgccg aggtcatcac cctgtgccgc
    26161 tcgggcagtg agcgcggtga gcccagcgcg cgtctgctgc gcgaaaacgg cctgccgaac
    26221 gcgcgctacg tgatcaacgg ctttcagggc gcggcgctca aggacgggcc gcaggccggc
    26281 ctgcgcatcc agaacggctg gcaaaacagt ggcctgccct ggagcgccaa gatgagtccg
    26341 ggcaaggtgt accgcgtcga ccgccagccc ggcaactgag tcgccggtac cagtagggtg
    26401 cgggcagggt gctgaccgcg ctataatttc aaggctttgc gtgttgcaga gcgcacgtgg
    26461 cgtgcagttc cggcgcgcca cgcaagtcaa accctttgga acgaggacat tgagatgatc
    26521 tccaaggaga acaaggccgc cgtggtcaaa gacaacgcgc gcgccgccaa tgacaccggc
    26581 agccccgaag ttcaggtggc gctgctgacg gcgcgcatca acgagctgac gccgcacttc
    26641 aaggaacacg ccaaggacca ccacggccgc cgcggcctgc tgcgcatggt cagccgccgt
    26701 cgcaagctgc tggactacct gaaggccaag gacgccgacc gctacctggc cctgatcgcc
    26761 aagctgggtc tgcgcaagta agcccgtgat gagcgcaaac gcctgacgcc tcccagggct
    26821 caggcgtttt tgtctttttt caacccggag cgcggggttt tcagaagctg aagctgtgtc
    26881 attccacgac ctttcgtcct tcaccggcag gttctggaat ggcatcgtgt tctgaaacaa
    26941 atccggctcc aacgcttgtg cagcaagcgc aagcagctat caatatagga gcaatcatga
    27001 gcatgttcaa caagatcacc aagtcgttcc agtggggcga caagacggtg gtgatggaaa
    27061 ccggcgagat cgctcgccag gccaccggtg ccgtgctcgt ggacatcgac ggcaccgtga
    27121 ttctggccac cgtggctgcc agcaagagcg ccaagccggg gcaggacttc ttcccgctga
    27181 cggtcgacta catcgaaaag acctacgccg ccggcaagat cccgggcagc ttcttcaagc
    27241 gcgaggccaa gccgagcgag ctggagacgc tgacgtcccg cctgatcgac cgcccgatcc
    27301 gcccgctgtt ccccgaaggt ttcttcaacg aagtgcatgt ggtgatccac accgtctcgc
    27361 tcaaccccga ggtggatgcc gacatcgcgg ccatgatcgg cgtcagcgct gcgctgtcga
    27421 tcagcggcat cccgttcaac ggcccgatcg gtgccgcgcg ggtcggttac atcaacggcg
    27481 aatacgtgct caacccgggt ccgacggcgc gcaagagcag ccagctcgac ctggtggtgg
    27541 ctggcaccga ggccgccgtg ctgatggtgg aaagcgaagc cgatcaactg tccgaggacg
    27601 tgatgctggg cgccgtggtg ttcggtcacc agcagggcca gatcgccatc aacgccattc
    27661 acgagctggt gcgcgaagcc ggcaagccgc tgtgggccga caacggcacc tgggtgccgg
    27721 aagccaagga ccaagccttc atcgcccagg tcaacgccgc cgccgagggc ccgctgcgcg
    27781 ccgcctacca gattcgcagc aagcaggcgc gcacccaggc ttgccgcgag gcctatgccg
    27841 gcgtcaaggc cgcgctgacc gagcagggcg tggcgttcga cccggtcaag ctcgacaacc
    27901 tgctgttcga gatcgaggcg cgcatcgtgc gcggccagat cctggccggc gagccgcgca
    27961 tcgacggccg cgacacgcgc accgtgcgcc cgatcgaaat ccgcaacagt gtgctgccgc
    28021 gcacacacgg ctcggcgctg ttcacgcgtg gcgagacgca ggccctggtg gtgaccacgc
    28081 tgggcaccga gcgcgacgcg cagcgcatcg acgcgctggc cggcgagttc gaggaccgct
    28141 tcctgtttca ctacaacatg ccgccgtttg ccacgggcga ggtgggtcgc atgggctcga
    28201 ccaagcgccg cgaggtcggc cacggtcgcc tggccaagcg cgcactgatc ccgctgctgc
    28261 cagccaagga tgagttcccc tacaccatcc gcgtggtgag cgagatcacc gaatccaacg
    28321 gctccagctc gatggcctcg gtgtgcggtg gctgcctgtc gctgatggac gccggcgtac
    28381 ccatgaaggc gcacgtggcc ggcatcgcca tgggcctgat caaggaagac aacaagttcg
    28441 ccgtgctcac cgacatcctg ggcgacgagg accacctggg cgacatggac ttcaaggtgg
    28501 ccggcaccac cgccggcatc accgcgctgc agatggacat caagatccag ggcatcacca
    28561 aggagatcat gcaggtggcg ctggcgcagg ccaaggaagc gcgcatgcac atcctgggca
    28621 agatgcagga ggccatgggc caggcgcgca ccgagatctc gagctacgcg cccaagctct
    28681 acacgatgaa gatcaacccc gagaagatcc gcgacgtgat cggcaagggc ggcgccacca
    28741 tccgcgccct gaccgaagaa accggcacca ccatcgacat cgccgaggac ggcaccatca
    28801 ccatcgccag caccgacgcc gccaaggccg accacgcccg gcgccgcatc gaggagatca
    28861 ccgccgaggc cgaagtgggc aaggtctacg aaggcccggt caccaagctg ctggagttcg
    28921 gcgccctggt caacatcctg cccggcaagg acggcctgct gcacatcagc cagatcgccc
    28981 acgagcgcgt cgaaaaggtc agcgactacc tgcaggaagg ccaggtggtc aaggtcaagg
    29041 tgctggagac cgacgacaag ggccgcatca agctgtcgat gaaggcgctg ctggagcgcc
    29101 cggcgcatct ggaaggcagc gacgagcgtc cgccgcgcgg cgatcgtccc ccgcgcggtg
    29161 agcgtggcga gcgcagggag cgtgcgccgc gtgaggatcg cggtgagcgc gaagagcgtc
    29221 cggcacccgc cgccggcgca cctgaggccg cccccgagct gccgtcgccg cagatctgat
    29281 tgctatataa agcatagctt ttggcgcagt acggacgagc gctggagccc tatctggcca
    29341 tgaaatgcat cgagatccgc gaacccggcg agccggccgt gctggtgccg gccgagcgcc
    29401 cgctgccgca ggccggcccg ggtgagctgc ggatccgcgt cagcgcctcg ggcgtcaatc
    29461 gcccggacgt gctgcagcgc gccggccact acccggcgcc gcccggtgcg tccgacctgc
    29521 ccgggctgga agtggccggg gtgatcgagt ccggcgacgc cgctgcgctg tctgcggccg
    29581 gcttgcagct cggccagcgc gtgtgcgcgc tggtggccgg tggcggctat gccgaggttt
    29641 gcgtggcccc ggtggcgcag tgcctgccgg tgcctgcggg ctgctccgac gtcgaggcgg
    29701 cggcgctgcc ggaaaccttt ttcactgtct ggagcaacgt gttcgaccgc gggcgcctgc
    29761 aggccggcga aaccctgctg gtgcagggcg gcaccagtgg catcggcgtc accgccatcc
    29821 agctggccaa ggctgcgggc gccaccgtga tcgtcaccgc cggttcggac gacaagtgtg
    29881 ccgccgcact ccgcctgggc gccgaccacg ccatcaacca ccgcacgcag gacttcgcgc
    29941 agcaggcgct gcagctcacc ggcgggcgcg gcgtcgacgt catcctcgac atggtggcgg
    30001 gcgactacgt gcagcgcgag gtcgaatgcc tggccgaaga cggtcgcctg gtcatcatcg
    30061 ccgtgcaggg cggcacgcgc agccagttca acgccggtct ggtgttgcgc cggcgcctga
    30121 cgatcaccgg ctccaccctg cggccacgcc cggtcgcgtt caaggccgcc attgcgcagg
    30181 ccctgcgcca gcgcgtctgg ccgtggctgg agcaggggcg tgtcaggccg gtgattcacc
    30241 aggtatggcc ggcgctggag gcgggcgatg gcctgcccag cggcgctgcc cgggcccatg
    30301 ccctgatgga aagcggtcag ctgatcggca agcttgtgct gacctggagc caaccatgag
    30361 cgaaagaaag aaactcgttg ccggcaactg gaagatgaac ggcagtctgg cggccaacca
    30421 ggcgctggtg caggcgctgc gccagggtgt cggccagccg gcgtgcgacg tcgccgtgtg
    30481 cccgccctcg gtctatctgg ggcagctgca gcaactgctg gcgggccaga cggcgattgc
    30541 gctgggcgcg caggacctgt cgcagcacga aggcggtgcc ttcaccggtg acgtctcggc
    30601 caccatgctg aaagacttcg gcgtgcgcta cgccatcgtg ggccacagcg aacggcgcca
    30661 gcaccagggc gagaccgacc tgcaggtggc gctcaaggtc aggcgtgcgc tggcggccgg
    30721 catcacgccc atcgtctgcg tcggcgagac gctgcgcgag cgcgaagagg gcctgaccga
    30781 attcatcgtc aagcgccagc tgtcggcggt gctccatctc aacggtcacg ccatcaatga
    30841 aaccgtggtc gcctatgaac ccgtgtgggc catcggtacc ggccagaccg ccacgccggc
    30901 gcaggcgcag agcgtgcatg cggtgctgcg tgcgcaactg cgcgccgccg gtgcccaggc
    30961 cgaacacgtg cgcatcctct acgggggcag catgaacgcg ggcaacgccg ccgagctgct
    31021 ggcgcagccc gacatcgacg gcgggctgat cgggggcgcc gccctcaagg ccccggattt
    31081 cctgaccatc gtggccgccg ccgcatgacg ggcgtgcgcc acatgctatt gattttggag
    31141 taactggaat gaatgtcatc ttgaacctga tcctggtggt gcagctgctg tcggcactgg
    31201 cgatgatcgg tctgatcctg gtgcagcatg gcaagggtgc cgacatgggc acggccttcg
    31261 gcagcggcgc ctcgggcagc ctgttcgggg ccaccggcgg cgccaacttc ctgtcgcgca
    31321 ccacagcggt gctggccacg gtgttcttcg tctgcaccct ggcgctggcg tatttcggct
    31381 tcagcgtgcg cagcgcgccg gccgacagtg gcagcgtgct ggaccgtgcc gcggtcacgg
    31441 cgccggccgc cggtgcctcg gcggcggcgc ccgccaccgc ggccgacgcc atcccgtccg
    31501 gcggcacgcc ggcaccggcc accgagccgt ccaggcccgc cacaccggcg gcaccggccg
    31561 ccagcggagc gggtcagatt ccgggccagt aaggggctcg tcagcccttg aaaaatcgac
    31621 cgtttcctct ctgtaacggc cggttttcag ggtaaactct tagctgctcc tgcgagtgcg
    31681 aaggcgttca cgctgacgtc gcgcaagagc cacccaatgc cgtcgtggtg aaattggtag
    31741 acacgctatc ttgagggggt agtggcgaaa gctgtgcgag ttcgagtctc gccgacggca
    31801 ccagaacgac aacgaaccgc gggtgtccca ggtccgtggt tcacccagac gaagaagccc
    31861 gatgaatctc gctcagtatc tccccgtcct gctgttcatc ctggtgggtc tggctgtcgg
    31921 ggctctcctg ctgggcatgg gcaacttcct caagccgcac aaggccgatg cggccaagaa
    31981 ctcgccctac gaatgcggct tcgaagcctt cgaggacgcg cgcatgaagt tcgacgtgcg
    32041 ctactacctg gtggcgatcc ttttcatcct gttcgacctc gagatcgcgt tcctctttcc
    32101 ctgggcggtg tcgctgcgtg aggtgggcct ggtcgggttt gttgccgtgc tcatctttct
    32161 ggccatcttg gtggtcggct tcgtctacga gtggaagaag ggcgcgctcg attgggagtg
    32221 agccgcgcgc ccggcgcgcg ccatgacagg acatccgcac atgaattcag agtttcagca
    32281 aaagggcttc ttgctcaccg gcgtggacgc ggcggtgaac tgggccaaga cgggctccat
    32341 ctggccggtg actttcggtc tggcctgttg cgcggtggag atgatgcacg ccgccgcatc
    32401 gcgctatgac attgcccggt ttggcgccga agtgtttcgc gccagtccgc gccagtccga
    32461 cctgatgatc gttgccggca ctctgtgcaa caagatggcg ccggcactgc gcaaggtgta
    32521 tgaccagatg gccgagccgc gctgggtgct cagcatgggc tcctgcgcca acggtggcgg
    32581 ctattaccac tacagctatt ccgtggtgcg cggctgcgac cgcgtggtgc cggtggacgt
    32641 ctacgttccg ggctgccccc cgacggccga ggcgctgctg tacgggatca tccagctgca
    32701 gcagaaggtg cgccgcaccc acaccatcgc gcgggtatga gcatgaccaa gcacgccatt
    32761 gcccctgaag ttctgaacga caccgtggcc agcgcgctgg gcgagctggt gcagcacatc
    32821 gacgtggccc gaggtgaggt cacggtcagt gtgtcggcgg gcgaatacca ccaggtgatg
    32881 cgcctgttgc gcgacgccgc cgggtgccgt ttcgaacagt tgatcgacct gtgcggggtg
    32941 gattactcca cctatggcga tggccgttgg gaaggcccgc gctttgccgt cgtcgtgcat
    33001 ctgctgtcgg tcagtctgaa ccagcgcctg cgtgtgcgtg tgttctgcac cgacgatgaa
    33061 ttcccggtca tccgctcggt caccgacctc tggagcgggg ccgactggtt cgagcgcgag
    33121 gccttcgacc tctacggcat cgtgttcgag gggcacgccg acctgcgtcg catcctgacc
    33181 gactatggct tcatcggtca tccgttccgc aaggatttcc cgctgaccgg tcatgtcgag
    33241 atgcgctacg acgccgagcg ccagcgcgtg atctacgagc cggtgacgat cgagccgcgc
    33301 gaaatcacgc cgcgcgtgat ccgcgaagac aactacggcg gcctgcactg accatcatgg
    33361 cggacatcaa gaactacacc ctcaacttcg ggccgcagca cccggcggcg cacggcgtac
    33421 tgcgtctggt gctggagatg gacggcgagg tgatccaccg tgccgacccg cacatcgggc
    33481 tgttgcaccg cgccaccgag aagctggccg agcacaagac ctacatccag tcgctgccct
    33541 acatggaccg tctcgactac gtgtccatga tgtgcaacga gcacgcctac tgtctggcga
    33601 tcgaaaagct gctcggcatc gaagtgccga tccgggcgca gtacatccgc gtcatgttcg
    33661 ccgagatcac gcgcctgctc aaccacctga tgtggctggg ttcgcacggc cacgactgcg
    33721 gcgcctcgac catgctgctc tacaccttcc gtgagcggga ggacctgttc gacatgtacg
    33781 aagcggtgtc gggcgcgcgc atgcacgcgg cgtatttccg ccccggtggc gtgtaccgtg
    33841 acctgcccga cagcatggcg ctgtacaagt ccagcaagat ccgcagcagc aagtcgctgg
    33901 acgcgatcaa cgccaaccgc cagggttcgc tgctggactt catcgacgac ttcaccaagc
    33961 gtttcccggt cctgatggac gaatacgaga cgctgctcac cgacaaccgc atctggaagc
    34021 agcgcaccgt gggcatcggc gtggtgacgc ccgagcgcgc actcaacctg agcctgaccg
    34081 gccccatgct gcgcggctcc ggcatcgcgt gggacctgcg caagacgcag ccctacgacg
    34141 tctacgctgc cctggatttc gacatcccgg tgggcaagac gggcgacagc tacgaccgct
    34201 acctggtgcg cgtgcaggaa atgcgcgaat ccaaccgcat catcaaacag tgtgtcgact
    34261 ggctgcgtgc caaccctggt ccggtgatcg tggacaacca caaggtggcg ccgccgccgc
    34321 gggaacgcat gaagtcgggc atggaggacc tgatccacca cttcaagctg ttttccgaag
    34381 gcatgcacgt gccggagggc gaagcctacg cggcggtcga acaccccaag ggcgagttcg
    34441 gcatctacct catcagcgat ggcgccaaca agccctatcg gatgaagatc cgggcgcccg
    34501 gttttgccca tctgtccgcg ttcgatgagc tgaccaaggg tcacatgctg gccgacgccg
    34561 tggccatcat cgggacgatg gacgtcgtgt ttggagagat cgatcgatga gcagtgcagt
    34621 tgccccttcc actgcgacac cgttttcgca agccacgctg gcgcgttttg cgcgcgaggt
    34681 cgccaagtac cctgccgacc agaagcagtc ggccgtgatg gcctgcctgg ccatcgtgca
    34741 gcaggagcag ggctgggtca gccccgacag cgaacgggcg gtggcggcct acctgggcat
    34801 gccggtgatg gccgtgcatg aggtcacgac cttctacaac atgtacaacc agcagccggt
    34861 cggccgcttc aagctgaacg tgtgcaccaa cgcgccgtgc atgttcgccg gcgggccgca
    34921 ggcgctggag cacctgtgcg agacgctcgg cgtgcagccg ggtgaaacca ccgccgatgg
    34981 cctgttcacg gtgcaggcct gcgagtgcct gggggcctgt ggtgacgcgc cggtgatgct
    35041 ggtcaacgac cgccacatgt gcagcttcat gtccaacgac aagctggatc agctggtcaa
    35101 tggcctgcgc cacgcggagg actgagtcat ggctcgtgtg caagacatcc tcgcgcagtt
    35161 ccaggccagg ggcctggaga cctgcttcca cggtcgccac atccatccac agatctatgc
    35221 cgggctggac ggcaccaact ggggcctgaa ggactacgaa gcacgcggcg gctaccaggc
    35281 gctgcgcaag gtgctgggcc aggacggtgg gcccggcatg acgcaggacg aggtgatcgc
    35341 cgagctgaag gcgtcgagcc tgcgtggccg cggcggcgcg gggttcccga ccggcctcaa
    35401 gtggagcttc atgccgcgca acttcccggg ccaaaagtac ctggtgtgca actccgacga
    35461 gggcgaaccg ggtaccttca aggaccgcga catcctgatg cacaacccgc atgcggtgat
    35521 cgagggcatg gcgatcgccg cctacgccat gggtgccagc gtgggctaca actacatcca
    35581 cggcgagatc ttccgggtct acgaacgctt tgaagccgcc atcgagcagg cacgcgaggc
    35641 cggctacctg ggccagggca tcatgggcag cggcttcgat ttccagttgc acgcgcacca
    35701 cggcttcggt gcctacatct gcggcgagga aaccgcgctg ctggagtcgc tcgagggcaa
    35761 gaagggtcag ccgcgcttca agccgccgtt cccggccagt ttcggcctgt acggcaagcc
    35821 gaccaccatc aacaacacgg aaacctttgc ggccacgccc tggatcattc gccacggcgg
    35881 tcaggcctac ctggagtgcg gcaaacccaa caacggcggc accaagatct tctccatcca
    35941 gggcgacgtg gcgcgccccg gcaactacga aattccgctc ggcacgccgt ttgccaagct
    36001 gctcgaactg gccggtggcg tgcgcggcgg caagacgctg aaggcggtca tcccgggcgg
    36061 ctcgtcctcg ccggtgctgc cggccgacat catcatgcaa tgcacgatgg actacgactc
    36121 catcgccaag gccggctcca tgctgggctc gggtgccgtg atcgtgatgg acgagtcgcg
    36181 cgacatggtc gaaaccctgc gccgcctgtc gtacttctac atgcacgaat cgtgcggtca
    36241 gtgcacgccg tgtcgcgaag gcaccggctg gctgtggcgc atggtcgatc gcatccagga
    36301 gggtcagggg cgcgccgaag acctggacaa gctcgacaac gtggccgaga acatcatggg
    36361 ccgcaccatc tgcgcgctgg gcgatgccgc ggcgatgccg gtacgcgcca tgatcaagca
    36421 tttccggcac gaattcgcgg ccaagatcga cgccgcacag acgctgacga gctgaagcga
    36481 accgaatcgg acaagcccat ggttgaactc gaaatagacg gcaagaaggt cgaggtgcct
    36541 gaaggcagca tggtgatgca tgcggccgag aaggcggaca tatacatccc gcacttctgc
    36601 tatcacaaga agctgtccat tgccgccaac tgccgcatgt gcctggtgga cgtcgaaaag
    36661 gctcccaagc ccatgccggc ttgcgccacc cccgtgacgc agggcatggt ggtgcgtacc
    36721 aagagcgaca aggcgatcaa ggcgcagaag tcggtgatgg agttcctgct catcaatcac
    36781 ccgctcgact gcccgatctg cgaccagggc ggcgaatgcc agctgcagga cctggcggtc
    36841 ggctatgggg cctccggctc gcgctacggc gacgaaaagc gcgtggtcgc gcccaagaat
    36901 gtcgggccgc tcatttccat gaaggagatg acgcgctgca ttcactgcac ccgctgcgtc
    36961 cgcttcggcc aggagatcgc cggcgcgatg gaattgggca tgtccaaccg gggcgaacac
    37021 tccgagatcg agaccttcgt cgggcagtcg gtcgattccg aactgtcggg caacatgatc
    37081 gatatctgtc cggtaggcgc actcaccagc aaaccgtttc gcttcagtgc ccgtacctgg
    37141 gagctgtcgc gccgcaagag catcagcccg cacgactcga ccggcgccaa cctggtggtg
    37201 caggtcaaga accatcaggt gatgcgcgtc gtcccgttcg agaacgaggc cgtcaacgaa
    37261 tgctggatcg ccgatcgcga ccgcttctcc tacgaagcgc tgaacggcga gcaacgcttg
    37321 accaagccca tgctcaagca ggacggcgtg tggcgggagg tcgactggca gaccgcgctg
    37381 gagcacgtgg ccaacggctt gagcgagatc cgtgccagcc acggcgcggg ccgcatcggt
    37441 gccctggtca gtccgcacag cacgcttgaa gaactgcatc tggcggcttc gctggtgcgc
    37501 gggctgggca gcgaaaacat cgaccatcgc ctgcgccaca gcgaattccc cgcgcccgag
    37561 ggggtgcgct ggctgggtac ttcggtcgcc tccctgtccg agttgcaatc ggtgctggtg
    37621 gtggggtcga acctgcgcaa ggaccatccg ctgttcgcgc aaagaattcg gcaggcagcc
    37681 cgccatggct gtgcggtggc agctatcact tcaagagcat tgttgaagac cgacacggcc
    37741 tgggccatgc cgctgcgcgc cgtgcaggcg gtcgaagcgg gcgactgggc gcaggccctg
    37801 gccgatgtgg cgacggtgct ggcggccgac aagggcgtgc ccgccccggt ggctggtcag
    37861 gccaccgatt cggcccgcgc gctggccggc agcctgcagt cgggtgagcg caaggcgatc
    37921 ttgctgggca atgcggcggc acaccacgct caggcttcgg tgttgctggc cttgtgccaa
    37981 tggatcggcg agcagaccgg cgccaccgtg ggttggctca ccgaggcggc caacacggtg
    38041 ggcgcacagg tggtgcacgc cgtaccgggc aagggcggcc tgaacgccgc ccggatgctg
    38101 gcgggcgatg tggacgcgct gctgctgctg gcctgcgagc caggcgccga ttcggcgccc
    38161 ctgcagcggc agggctgcgg cctggtggtc acgctcagtc cgttcaaggc caacctggac
    38221 atcagcgacg tgctgctgcc gatcgccccg ttcaccgaaa cctcgggcac cttcatcaat
    38281 gccgagggca gggtgcagag cttccatgcc gtggtgcggc cgctgggcga tacgcgcccg
    38341 ggctggaagg tgctgcgcgt gctggccgac ctgctgcagg tggtgcaacc ggccttcgag
    38401 acctcgcagg aggtgctggc ccatgcgctg ggcggcagcg tgtccgagtt cgtgccggcc
    38461 gcacgcctga gcaaccgcac gacggccgca atccagatcc gcgcgggcgc tgtgcacgtt
    38521 ccccctgtgg gcatctacca gctcgacggc atcgtgcgcc gggcgccgtc gctgcagcag
    38581 accgccgacg ccctggaagg agcaagcgca tgatcgacgc catctacaac gtcggcctgc
    38641 acctgtttgc cggcagctgg tggagcggca tcgcctggcc ggtggtctgg agcctgatca
    38701 agatcgtcgt gctgctgctg ccgctgatgg gcgcggtggc gtacctgacc ctgtgggagc
    38761 ggcggctgct gggtttcatg caggcgcgtc tggggcccaa tcgggtcggt ccggccggct
    38821 tgctgcaacc catcgccgac gccgtcaagc tgctgaccaa ggaaatcatt cgtcccacgg
    38881 cagcggccaa ggggctgttc ttcatcggtc ccgtcatggc catcatgccg gcgctggccg
    38941 cctgggtggt aatcccgttc ggccccgagg ctgcgctggc caacatcaat gccggtctgc
    39001 tgctggtgct ggcgatcacg tcgatcgagg tctatggcgt catcatcgcc ggctgggctt
    39061 ccaactccaa gtacccgatc ctgggtgccc tgcgcgcatc ggcgcagatg gtcagctacg
    39121 agatcgcgct gggcttctgc ttcctggtgg tgctgatggt gtcgggcagc atgaacctga
    39181 ccgacatcgt gatggggcag gccaagggca tggcggccga caagggcctc ggcttcctgt
    39241 cctggaactg gctgccgctg ttgccgatct tcatggtcta cctgatctcg gtggtggccg
    39301 aggtcaaccg ccatccgttc gacatggccg agggtgaggc cgagattgtg gccgggcaca
    39361 tggtcgagta ttccggcatg ggctttgcca ccttcttcct ggccgagtac gccagcatgt
    39421 ggctggtgtc gatcctcgcc gtgttgatgt tcctgggcgg ctggttgcct ccgatcggtg
    39481 cgctggactt cattccggga tggatctggc tgggcatcaa gaccttcatc gtggtgtcga
    39541 tgttcatctg gatccgtgcc accttcccac gttaccgcta cgaccagatc atgcgcctgg
    39601 gctggaagat cttcatcccg gtgacgctca tctggctgat cgtcgtgggc ctgtggatgc
    39661 acagcccctg gaacatctgg cactgaccgc agggttcgtc atggcttccg tcactgcttc
    39721 accgttttcg ctccgcgatt tcctcaagag cttcgtgctc gtggaattgt tcaagggcat
    39781 ggccctgacc gggcgctacg ccttccggcg caaggtcacc atccagtacc ccgaggaaaa
    39841 aaccccgctg tcgccgcgct ttcgcggcct gcatgcgctg cgccgctacg acaatggcga
    39901 agagcgctgc atcgcctgca agctgtgcga ggccgtatgc ccggccatgg ccatcaccat
    39961 cgaggccggc gagcgcgccg acggttcgcg tcgcaccacc cgctacgaca tcgatctgac
    40021 caagtgcatc ttctgcggct tctgcgaaga gagctgtccg gtcgactcga tcgtcgaaac
    40081 ccatatcctc gagtaccacg gcgagaagcg gggtgacctg tatttcacca aggacatgct
    40141 gctggccgtg ggcgatcgct acgaatctga aatcgccgcc gccaaggcgg cagatgccag
    40201 gtaccgctga cccgcgctgg ctggcccacc actagacaag acccatggac gtcaagaccg
    40261 gctttttcta cgtgttcgcc ctggtgatgc tgttttcggc attccgggtg atcaccgcgc
    40321 gcaacccggt gcacgcggtg ctgtacctga tgctcacctt ctcgcaggcg gccggcatct
    40381 ggctgctgct gaaggcggag ttcctcggca tcgcgctggt gttggtctac ctgggcgcgg
    40441 tgatggtgtt gttcctgttc gtggtgatga tgctggacat ccacatcgac accgtgcggc
    40501 agggcttctg gcgccatgtg ccgctggcgc tggcgatcgg tgccttgatc acgctcgaaa
    40561 tggcggtggt gctgttcggt ggcttccgcc tcagcgaagc gccccaggtg cccgagacca
    40621 tgaccaatgc cgcggggcag gtggtgcagt actccaacac caaggtgctg ggtacgctgc
    40681 tctacaccaa gtacctgtat ccggtggaaa tcgccgcggt cctgttgctg atcgccatgg
    40741 tggcggccat cgcgttgacc ctgcgtgagc gcaaggacac caagaaggtc gatcccagcc
    40801 tgcaggtcag ggtcaaggcg agcgaccgcc tgcaggtgct caagctggcg cccacgcagg
    40861 cgccgctgcc ggtcgagccg cccgccgcgg ccatcgtgga ggaggtcaaa ccatgacgct
    40921 gacgctggcc cactttctga cgctgggcgc catcctgttt tctctcgccg tggtggggat
    40981 ctttctcaac cgcaagaaca tcatcgtgct gctcatggcc atcgaactga tgctgctggc
    41041 catcaacatg aatttcgtgg cgttttcgca ttacctgggc gatatgcacg gtcaggtgtt
    41101 cgtgttcttc atcctgaccg tggcggcggc cgaagcggcc attggcctgg cgatcctgat
    41161 gctgctgttc cgcgccaagt ccagcatcaa cgtggacgag atcaattcgt tgaagggtta
    41221 acagagtcat gagtcagacc ctttctgcag ccacactgct ggcggtgccg ctggcgcccc
    41281 tggcgggctc gctgatcgcg ggcatcctgg gcaccgcctt cggcggcaac cgcatcggcc
    41341 gcacggcctc gcacacggtc accatcctcg gggtgctgat cgcgttcgtg ctgtcggcgc
    41401 tgaccctgca aagcgtgatc gccggtgcac gcttcgaagg caccctctac gagtggatgg
    41461 tggtgggcgg actgaagatg gaagtcggct tcctgatcga cagcctgacc gccaccatga
    41521 tgtgcgtggt gaccttcgtg tcactgatgg tgcacatcta caccatcggc tacatggccg
    41581 acgacgatgg ctacaaccgc ttcttctcgt acatctcgct gttcacgttc tcgatgttga
    41641 tgctcgtgat gagcaacaac ttcctgcaac tgttcttcgg ctgggaagcc gtgggcctgg
    41701 tgtcctatct gctgatcggc ttctggttca accggccgac ggccatcttc gccaacctga
    41761 aggccttcct ggtcaaccgc gtgggcgact tcggcttcat cctcggcatc ggtctgatcg
    41821 ccgcctacgc cggcagcctg aactacgccg aggtgttcgg caaggcgggt cagcttgcgg
    41881 gcctgggctt tccagggacg gactggctgc tggtcaccgt gatctgcatc tgcctgttca
    41941 taggcgccat gggcaaatcc gcccagttcc cgctgcacgt gtggctgccc gactcgatgg
    42001 aaggtccgac acccatttcc gccctgatcc acgcggcgac catggtgacc gcgggcatct
    42061 tcatggtggc gcgcatgtcg ccgctgttcg agctgtcgga tgcggcgctg aacttcgtgc
    42121 tggtgatcgg tgcgatcacg gcgctgttca tgggctttct gggcatcatc cagaacgaca
    42181 tcaagcgcgt ggtggcctat tccaccctgt cgcagctggg gtacatgacg gtggcgctgg
    42241 gtgcgtcggc gtattcggtg gcggtgttcc acctgatgac gcatgccttc ttcaaggccc
    42301 tgctgttcct ggcggcgggc tcggtgatca tcggcctgca ccacaaccag gacatccgct
    42361 ggatgggtgg cgtgcgcaag tacatgccga tcacctggat caccttcctg ctgggctcgc
    42421 tggcgctgat cggcacgccg ttcttctccg gcttctactc gaaggacgcc atcatcgagg
    42481 cggtgcatgc cagcaagctg cccggcgccg ggttcgcgta cttcgcggtg ctggccgggg
    42541 tcttcgtcac cgccttctat tcgttccgtc tgtacttcct ggtctttcac ggtcaggagc
    42601 gctacgacca gaaccccgac gcccaccacc atgacgatca ccacggccat ggccacgatg
    42661 cgaaaccgca cgagacgccc tgggtggtct ggttgccgct ggtgctgctg gccattccct
    42721 ccgtggtgat cggcgccatc acgctgatgc cgatgctgtt cggcgatttc ttccggctca
    42781 acgacgtgat ccatgtggat gcggcgcgcc atcctgccat ggcggtgctg gccgagaaga
    42841 tccacggctg ggtcccgatg gcgctgcacg gcttcgtggc accgccgttc tggctggcgc
    42901 tggctggcgt ggcatgcgcc tactacatgt acatggtgaa cccggccctg ccggccgcca
    42961 ttcagcgcgc cttccggccc gtctacagcc tgctggagaa caagtattac ctggactggt
    43021 tcaacgaaaa cgtggtggcg cggggcgcgc gtctgctcgg caccggtctg tggaaggcgg
    43081 gcgatcgtgc gctgatcgac ggcctgctgg tcaatggctc ctggcgcctg gtgggttggg
    43141 tgtccggcat ggtgcgctgg atccagtcgg gctacctgta tcactacgcc ctggtgatga
    43201 tcctgggcat cttcgtgctg atgacgtact tcgtctggct caacaagtaa ggaagacggg
    43261 ataagaacat gggttggttg agccttgcca tctggacgcc gatcgtcttc ggcgccgtgt
    43321 tgctggcgct gggacgggac agccaggcac agctggtgcg ctggattgca ctcatcggct
    43381 ccgtggtcag cctgctgatc acgctgccgc tgtacaccgg ctttcgcctg ggcaccgccg
    43441 agatgcagtt cgtcgagaag atgccctgga tctcgcgctt caatgtcaat tactaccttg
    43501 gcgtggacgg catctcgctg tggttcgtgc tgctgacggc gttcatcacc gtgatcgtcg
    43561 tgatcgccgg ctggcaggtg atccagcgca aggtgaatca gtacatggcc gcgttcctga
    43621 tcctctcggg tctgatggtc ggcgtgtttg cggcgctgga cggggtgctg ttctacgtct
    43681 tcttcgaggc cacgctgatt ccgatgtacc tgatcatcgg catctggggc gggccacgca
    43741 agatctacgc cgcgttcaag ttcttcctct acacgctgct gggctcgatg ctgatgctgg
    43801 tggcgttcat ctacctgtat gcggtctcgg gtggcagctt cgacgtgctc acctggcaca
    43861 agctgccgtt gtcggcaacg gcgcagacgc tgctgttctt tgccatgttc gcggcgtttg
    43921 ccgtgaaggt gcccatgtgg ccggtgcaca cctggctgcc cgacgcgcac gtggaagcgc
    43981 ccaccggcgg ttcggtggtg ctggccgcca tcatgctgaa gctgggtgcc tacggttttc
    44041 tgcgcttttc catgccgatc ctgcccgacg cctcgcgcga gtgggcgtgg ctgatgatcg
    44101 cgctgtcgct catcgcggtg atctacatcg gtctggtggc gttggtgcag caggacatga
    44161 agaagctggt ggcctattcg tcggtcgctc acatgggctt cgtgaccatg ggtttcttca
    44221 tcttcaacga cctgggcgtc accggtggcc tggtgcagat gatcgcgcac ggcttcgtgt
    44281 ccggtgccat gttcctgtgc atcggcgtgc tgtacgaccg ggtccattcg cgcgagatct
    44341 ccagctacgg cggggtggtc aattcgatgc cacgcttcgg cgccttcgcg ctgctgttct
    44401 tcatggccaa ctgcggtctg ccgggcaccg ccggcttcat cggtgagtgg atggtgatcc
    44461 tggccacggt gcgcgccaac ttctggctcg gtttgctgct ggccacggcg ctgatcaccg
    44521 gtgccgccta cagcctgtgg atgttcaagc gggtctactt cggtgcggtc gccaacgacg
    44581 acgtccgtgc catgcccgac atcgatgggc gcgagttcct ggtgctggcg ctgctggcca
    44641 tcgccgtgct gtggatgggc ctgtatccga aacccttcac cgacgtcatg gacgcttcgg
    44701 tggtcaacct gctccagcac gtggcgaatt ccaagctgaa ctgaggacga ggacatgatt
    44761 gacagattca gttggatggc cgtctatccc gagatcgtgc tgctggtcat ggcatgcgtg
    44821 atcacgctgg tggatctgct ggaccgcaac ccgcgccggc tggcggccta tgtgctgtcc
    44881 atcctgacgc tgctggtgct ggcggtgctg accggcacct acgcgcgggg cggccagacc
    44941 tggtacgggt tcgagggcat ggtggtcagc gacccgatga ccaactggct caagtgcttc
    45001 gcggcgctgg cggtggccgt ctcgctggtc tacgcgcgca cctacgtcac cggtcgtggc
    45061 atgttgcgcg gcgccgagtg gtacgtgctc agtctgttgt cgttgctcgg catgtacgtg
    45121 ctgatctcgg ccaacaactt cctgctggtc tatctgggcc tggagtgcat gacgctgtcc
    45181 ggctacgccc tggtggcgct gcggcgcgac cacgtgacct cgatcgaggc cgccatgaag
    45241 tacttcgtgc tgggcgccat ggcttccggt ttccttctgt atgggctgtc catgctctac
    45301 ggcgccaccg gcacgctgga catcggcggt gtcttcaagg ccgtggcctc gggtcagatc
    45361 aagcaccagg tgctggtgtt cggcgtggtg ttcgtggtgg ccgggctggc gttcaagttc
    45421 ggtgccgtgc cgtttcacat gtggctgccg gacgtctacg aaggcgcgcc caccgcggtc
    45481 acgctgatga tcggcggcgc gcccaagctg gccgcgttcg ccatggccgt gcgcctgctg
    45541 gtggagggcc tgctgccgct ggccatcgac tggcagcaga tgctggcggt gctggcggtg
    45601 gcttcgctgc tgatcggcaa cctggcggcc atcgcgcaga ccaacctcaa gcgcatgctg
    45661 gcgtattcca ccatcgcgca gatgggcttc gtgctgctgg gcctgatggc gggcgtggtc
    45721 aaccacagtg cgctggcggc cgccaacgcc tacagctcgg ccatgttcta cgtcgtggcc
    45781 tatgtgctga ccacgctggt gagcttcggc atcatcctgc tgctgtcgcg cgagggcttc
    45841 gaaagcgagg agatcgtcga tttcgccggc ctgaaccaac gcagcccgct gtacgccggc
    45901 ctgatggcca tctgcctgtt ctcgctggcc ggcattccgc cgatggtcgg cttccaggcc
    45961 aagctggcgg tgctgcaggc cctggtcagc accggcctgc cgtcctacat tggcctggcg
    46021 gtgttcgccg tcatgatgtc gctggtgggt gcgttctact acctgcgcgt ggtcaaggtg
    46081 atgtacttcg acgagccgct gtcgagcgcg ccgatccagg cgccgttcga catgcgcgcg
    46141 gtgctgtccg tcaacggcct gctgctgctg gtgctgggca tcgtgccggg ctcgctgatg
    46201 gcgctgtgcg ccaaggccgt ggtccagatg cttgcgactt gaggatgtcg cagaacctct
    46261 ccatctggct gctgattttg ctggccctgg tggccgccaa tctgccgttt gtcaaccatc
    46321 gtctgatggc ggtgcttccc ctgcgcacgg cggccaaggg tctggcgtgg cgcctgctgg
    46381 agctgctgct ctggtatggc ttcgtgatcg gcgtcggcat ggcgatcgag cgcgcccagg
    46441 gtcagaacca gccccagggc tgggagttct atgccgtcac ggcggcgctg ttcctgaccc
    46501 tggcgtttcc gggcttcgtc tatcgctacc tggtgcgtcg ggggcgttga tggcgtccga
    46561 tttgcgcgag acccgcgtcg gtgggcagga gctgttcaag ggcgacttcc tgcacgccat
    46621 gcgtgaccgg gtgcggctgc ccgatggtca gctcacacag cgcgaataca tcgtgcaccc
    46681 aggcgcggtg atgatcgtgc cgtggctgca ggggccggat gggtcgctgc aactggtgct
    46741 ggaacgacag tatcgctatc cggtgcagca gaccatcatc gagtttccgg ccggcaagct
    46801 cgatccaggc gaagcgcctc tggtctgcgc tcagcgcgaa ctgcgcgagg agaccggcta
    46861 caccgcccgc gagtgggcgc gggccggcct gttgcatccg gtggtctcct actcgaccga
    46921 gttcatcgag gtttggttcg cccgtggcct gcaggccggc gagcggcggc tcgacgaggg
    46981 tgagttcctc gaggtcttca cggcttcccc cgagcagttg tatgcctggt gccgggatgg
    47041 ggagatcacc gacgccaaga ccctgatcgg ggccttgtgg ctgcagaatg tggccagcgg
    47101 cgcctggtcg ctgaactggc aaccggccga tccggcagca tgaccccgca atgaaagtcc
    47161 tcaacctgca atgtgcccac ggccacgcgt tcgagggctg gttcgcgtcc gaggacgact
    47221 tccaggacca gcgcacgcgg cagcttgtcg aatgcccgct gtgcggtgac gtcgaggtga
    47281 ccaagctgcc cagcgccccg cggctcagcc tgagcggcgc cagtgcggcc gacaccgcat
    47341 cgtctgcgtc ggcaaccggg ccgaccgctg cagcggcctc ggtggccaac ctgccaccgg
    47401 cggcgctgca ggccgcctgg atgcacctgg tgcgccaggt catggcacgc accgaagacg
    47461 tgggcgagcg ctttgccgaa gaagcgcgcc gcatgcacta cggcgaaacc gaagaccgtg
    47521 gcatacgcgg gcaggccacg ccggccgaga ccgaagcgct gctggaggag ggcatcgccg
    47581 tcatgccgct gcccctgcca ccgggcttca agggtacgtt gcaatagtcc tagatgactt
    47641 cgatagctgc cggcgctttc ctgaaggggc cggagcttga ttgggcaccg agggccgcgc
    47701 ctggccgcca gcctcgacca aagccggctg tggggccccg tctcagctgc aactgggggc
    47761 gttgacgatg gcgcgcagcg cttcggtgtt caggatgcgc acgtggcgct gcttgacttc
    47821 gacgatgcct tcctcggcga acttcgagaa ggtgcggctc acggtttcca gcttcagccc
    47881 caggtagctg ccaatttcct cgcgcgtcat gcgcagcacc agctccgact gcgagaagcc
    47941 gcgcgcatgc aggcgttgca ccaggttcag caggaaggcc gccaggcgct cttcggcgcg
    48001 catgctgccc agcagcagca tcacgccatg ctcgcgcacg atctcgcggc tcatgatctt
    48061 gtgcacgtgc gactgcagcg cctgcacctc tcgcgacagc tcttcgatgc ggtcgtaggg
    48121 catgacgcag acttcggcgt cttccagcgc caccgcatcg caggtgtggc ggtcgcccac
    48181 gatgccgtcc aggccgatca cctcgccggc catctgaaag cccgtcacct gatcgcgccc
    48241 gtccgcggtg gcgacgcagg tcttgaagaa gccggtacgg atggcgtaca gcgcggtgaa
    48301 gcggtcgccg ttggcaaaca gcgaggtgcc gcgcttgacc ttgcgccgag ttgccaccag
    48361 gtcgtcgagc ttgcccattt ccgtgtccga caggcccatc ggcatgcaca gctcgcgcag
    48421 gttgcagttg gagcaggcga ctttgatggc ttgtggagtc atgcgggcgt cgggtggtca
    48481 gtgccgggca cggtacatgc gcggtgacgc tgcattctag gcgatcgggg ctggtgccgg
    48541 tggattcttg atgcggctca aaatccatcc gtgccggaac gggtacttga tcgccgtcaa
    48601 ggcgcgatcg cgtcagcccg gcgacagttg agcctgacgt caaaggactt attggatgca
    48661 agccattacc cccgaacttc tcaccaagtt cgacgtgccg gggccgcgtt acacctccta
    48721 cccgaccgca gatcgctttg tcgaggcctt cggcgagccc gactacctgc gggcgctgga
    48781 tcagcggcgc accgggccgg tggccggtgc gcggccgctg tcgctgtacg tgcacatccc
    48841 gttctgcgaa tcgctgtgct actactgcgc ctgcaacaag atcatcacca agcaccacga
    48901 ccgcagcgcg ccctacctgc gctacctgag caaggaggtg gacctgcacg tgcagcacct
    48961 cggcgccggc cagtcggtca gccagctgca tctgggcggg ggcacgccca ccttcttcag
    49021 cgacgacgag ctgggcgagc tgatgagcat gctgcgccgc aacttcaacc tggtggcggg
    49081 cggtgaatat tccatcgaag tcgatccgcg caccgtggac gcaacgcgcc tgcagacctt
    49141 ggcgcggctg ggcttcaacc gcctgtcgtt cggcgtgcag gacttcgatc cgctggtgca
    49201 gaaggcggtg caccgcattc agccggccga acaggtgttc gccctggtgg acgccgcgcg
    49261 gcgcatcggc ttcgagtcga tcaacgtcga tctgatctac ggcctgccca ggcagacgcc
    49321 tgaatccttc gatcgcacgc tggcccaggt ggcccagttg cgacccgacc gcattgcgct
    49381 gtacggctac gcgcacctgc ccgagcgctt caagccgcag cggcgcatcg tggcggtgga
    49441 cctgccgggc gccgccaaca aggtggccat gctggcgcgc gcgctggcgg cgttcgacgc
    49501 cgccggctac gactacatcg gcatggacca cttcgcgctg cccgacgacg cgctggccgt
    49561 ggccaagcgc caggggcggt tgcaccgcaa cttccagggc tacagcacgc agcccgactg
    49621 cgacctgatc ggtctgggcg tgtcggcgat cggcaagatc ggtgccacct acagccagaa
    49681 cgccaaggcg ctggaagact accaggacct gctggaccag ggccacctgc cggtggtgcg
    49741 cggcctggcg gtgtcgcgcg acgacctgct gcgccgcgcc gtcatcatgg ccctgatgtg
    49801 ccagggcgag gtgctgtacg aatcgatcga gctggcgcac ctgatccgct ttcaggacta
    49861 cttcgccagc gaactggaaa ccctgcgcgg catgcaggcg cagggcctgg tcaggctcac
    49921 cgacaccggc atcgaagtca cgcccatggg ctggttcttc gtgcgcggcg tggccatggt
    49981 gttcgacaag tacctgcagg ccgaccgcac ccgggcgcgg ttttctaaaa tcatctgatg
    50041 caatcttccc tggccgtgac cgccctggtc atggggctgg ccggcggtcc gcactgcatc
    50101 gccatgtgcg gtgcggcctg cgccggcatc gggcaggcgg cggcgccgcg aagcacccag
    50161 gccctggcgc tgttccagct cggccggctg accggctatg cggcgctggg cgcgctggcc
    50221 gccgccacca tgcagggcct gggctggctc acggtgcagt cggccgcctt gcgcccggtg
    50281 tggaccatgg tgcacgtggc tgcggtgctg ctgggtctgt cgctgctggc gttcgcgcgc
    50341 cagccggtct ggcttgaagc cggcgcgcgg cgcgtctggg cacgcgtgcg caccgccacg
    50401 cagtcgctcg gcctggtggc tccgctgggc ctgggtgtgg cctgggcgct gctgccctgc
    50461 gggctgctgt actcggcgct gatggtggcg gccctggccg gcagcgtggg cggtggcgcg
    50521 gccgtcatgg ccttgttcgc cctcggctcc ggcctgtggc tgggtctggg gccgtggctg
    50581 ctgctgcggc tgggccggtc cgggcgcggc gcctggagca tgcgcgtggc cgggttggcg
    50641 ctggccgcca cctcggcctg ggggctgtgg atggggctgg tgcacgacca ggcgccctgg
    50701 tgcgtggtgc ccacgccctg acaattgcta ttatttgggt agctgctggc gcttgctgca
    50761 caagcgctgg ggccagaaaa gcctgtgatt ctggccgggc ggccccggtc agcccgtcag
    50821 cgcgccgatg cccttgttca gcatgtacaa ggccagcacg aacaacacgc cggcaaaggc
    50881 gcgcttgagc ttcttcaccg gcagcgcgtg cgcggccctg gcgcccaccg gcgccatcag
    50941 cacgctgcac gaagcgatca ccaccagcgc cggcagccac aggtaaccga acgaataggc
    51001 cggcagcccc tgcaggccct ggccggcgac gatgtagccc agcacgttcg acagcgcaat
    51061 cggaaagccc agcgccgcgc tggtggccac cgcgttgtgg atggccacgt tgcaccaggt
    51121 catgaagggc acgctgacga agccgccgcc ggcgcccacc aggcccgaca gaaagccgat
    51181 cacgctgccg gccgccagct ggccggtggt gcccggcaac tggcggctgg cggccggctt
    51241 cttgtccagg tacatctgca cggccgagaa ggccacgaag ccggcgaaca cgaagtacag
    51301 cgccgccccc ttgagcagcg caaacacgcc caggcttgcc accaggccgc ccagcacgat
    51361 gccgggggcc aggcgcttga cgatgtccca gcgcaccgcc cccttcttgt ggtgcgcgcg
    51421 cacgctgctc atggaggtga agatgatggt ggccatcgag gtggcgatgg ccatcttcac
    51481 cgccaggccg ggcgccaccg cgcgccccac cagcatggtc gagatgaagg gcaccatgat
    51541 catgccgccg ccgatgccca gcaggccggc cagaaagccg gtggccaggc ccagcgcggc
    51601 cagttcgatg aaaagaagcg gttcgatcgg catcggggtg gtggcatcga gcttgcctgg
    51661 cgcggcacgg gcgtcaagca aaaaggccgg acacgcgccg gcctgggaaa tgaggtgtct
    51721 gcgaatgcca ccggaccgtc atcctgaacg cgggggttca ggtgggcgag gtcagactga
    51781 tcttgggttc atctgacgcg agacgatcac gccgaccaga cgatgttcca agcccgggct
    51841 catacgagca ttggttcaag gaaatataaa gcccggcatg caccgcagac ggctcgattc
    51901 tatcgcctcg ggcgtccgtc gctgcgcgtg ccgcccggcg ggctggggcg gccggcgcac
    51961 gcggctacag caactggccg tcgttgccca gcgcctggtc cagccgggcc agcagggcgc
    52021 ccagcgcggc ctggctggcg ggctcgtcgg cgcccgccgg ggtgtcggtg ggcgccgtgg
    52081 tggcggcatc ggcggggcgc ggcgcgtcgg tcagttcaaa ggccaggttc agcgccgcca
    52141 gcacggcgat gcggtcgcgc gccttgatgc ggccggcatc gcggatcttg cacatggcgg
    52201 catcgacccg ttgcaccgcc tgctgcaggc gctgctcgcc gccttccggc gccgccagca
    52261 ggtaggcctg gcccatgatg cgcacttcga cctgcttcat cgcgtggcct ccttgcctgc
    52321 gggtggcgta tcgggcgtgg cttcgccgct cagcacgccg gcggtggccg gcaggcgatc
    52381 gatcagcgaa tcgatgcgcg cgcgcgccgc catcaggcgc gagcgcaggt tgtcgcgctc
    52441 gtgcgtcagc gcctgcacct gctgggccag caggtcgttg gtgcgctgca gttcctcgtg
    52501 gcgcacgagc aggcgctcga cgcgctcgga cagctgttcg atcaggggcg gggtcgtcat
    52561 cgggagggca ccgtgtcgct gaaggcgccc cgattgtagg cggcgcgcga caggcgcccg
    52621 ctacaatcgc caccgttggt gctcgcgcgc cgtgactctc acggccgcag ttcaacggga
    52681 agcaggaggg catgccgacc gacccggtcg ccgcctaacc tgcgctgccc ccgcaacggt
    52741 aagcggatgg cgtcgtgacc cgtcacggcg ccgactcctg cctcacaagg tcactggcgc
    52801 cgcgtcctcg acgcgctgcc gggaaggcca ggcgatcacg gagccaattc cgccagcccg
    52861 gataccggcc aacgaggcga cgtcgcccgc gcagtgcccg ccaggcctgc cgtgcctcgt
    52921 catgtcgccc catttgccgg cggggaagcc ggccggggct gctgcatccc gtgtttcccg
    52981 tcatgtccct tcgtctgcaa tccttccggc cggctgcgct gccgctggcg ctcgcctgtg
    53041 ccttctgcgc ccatgcggcg caggacacca ccgtcgccca gctggccgcg cccagcctgc
    53101 ccgacaccgt ggtgaccgcc acccgcgtgg cccaacccct gaccgacgtg ctggccgacg
    53161 tcaccgtgct cggcgacgag cagatccgcg ccagcggcgc ggtcagcatc ggcgatctgc
    53221 tggcgcgcca gccggggctg gagctggccc gcaacggcgg cctcggcagc gccaccagcg
    53281 tgtacctgcg cggggctgaa acccgcttca ccgccgtcta catcgacggc atccgcatcg
    53341 actcgcaggc caccggcggc gccccctggg aggccatcgc gctgggccag gtcgaacgca
    53401 tcgaaatcgt gcgcggcccg gccgccgccg tgtacggctc ggacgccatc gcgggcgtgg
    53461 tgcagatctt cacccgcaag ggcgaaggcc cgttcgcacc ctatgtcggc gtgggcctgg
    53521 gcagccaccg cagcggcaag ctggaggccg gcttttcggg ccgcaacggc gcgctcgact
    53581 acgccctggg cgccgcgcgc gagctgagcc gcggctacaa cagcatgccg gcgggcaacc
    53641 ccgaccgcga cggctaccgc cagagctcgg ccagcggccg cctgggctgg aacatcaacg
    53701 acgcccaccg gctcgaactg agcggcaccc acaccgacat cgacgcccag tacgacggct
    53761 ttctgcccgg cctggacgac cacggcctga accgcctggg caccctgggc gccgcctggt
    53821 cggcaaagtg gagcaagcgc tacagcaccc gcctgtcggt gagtgaatcc gaccagcgct
    53881 acgaaaccgt gccttcgccc tatctcacca agacgcgcct gcgcaactac ctgctgcaga
    53941 acgaatggcg cgagggcgct cacctgatca ccgccacgct ggagcggcgc gaagaccatc
    54001 tggtcaacgc cccgatcgcg cagggcaggc accagaatgc catcgccctg ggctacggct
    54061 tcagcggcgg gccgcacacc ctgcaggtca acctgcgcca cgaccgcgac agcgagttcg
    54121 gcggccagac caccggtggc gtggcctacg gctacggctt tgcgcccggc tggcgcgcca
    54181 ccgtggcggc cggcacggcg tttcgcgtgc ccacgctgta ccagcgcttt tccgaatacg
    54241 gccagcctgc gctgcagccg gaaaagggcc gcaacgtcga gctggggctg cactgggcgc
    54301 agggcgacag ccgcttcggc gtcagcgtct ggcgcaaccg cgtcagcaac ctgatcgtgt
    54361 tcggcgcccc cggcccctgc gccggcgctt tcggctgcta cgaaaacgcc ggccgcgcgc
    54421 gcctgcaggg cgtgacgatc agcggcgcac accggctggg cggcgtcaac ctgagcgccg
    54481 cgctcgacct gcagcaaccc cgcaacacgc tcaccgacaa gctgctgccg cggcgtgcgc
    54541 agcgcctgct caagctcagc gccgatatcc gtgtgggcgg ctggacgctg ggcgccgaat
    54601 ggcaggccag cagccaccgc tgggacaacg ccgccaacac ccgtcgcctg gccggctacg
    54661 gcatcgtcaa cctgtacgcc agcaccaccc tggcgcgcga ctggcaggtg ctggcgcgcc
    54721 tggacaacct gggcgacagg gactaccagc tggcgcacgg ctacgccacg cccggccgca
    54781 gcctgttcgt cggcctgcgc tggacgccgc gctgagcgcg gcaagcgggg cagcgggcat
    54841 ggacgtcgcg cgcagcgagc tgatcctggg cggccagaag agcggcaagt cgcgccgcgc
    54901 cgaaagcctg gccgccgcct ggctggcggc cgcgcccacg caccgggcgg tgctgatcgc
    54961 caccgccggc gcgcacgacg ccgagatgcg cgcccgcatt gcccgccacc agcgtgagcg
    55021 cgccgcgcgc gtgcccgggc tggtcacgca cgaggcgccg cacgaactgg ccgccgccat
    55081 cgcccaggcc ggcggcgccg aaacgctgcg catcgtcgac tgcctgaccc tgtggctcac
    55141 gcagcgcctg atgccgctgc ccggtcatga atcccatggg cctgcggcgc ttgatgggca
    55201 agcgccggca gctatcatta ttgaagcact gcgaagctgc cccggcccca tcgtgctggt
    55261 cggcaacgaa atcggcctgg gcgtgatccc gctcggcgcc gaggtgcgcg ccttcgtcga
    55321 cgcgctgggc cagctcaacc aggcggtggc cgcgcgctgc cagcgcgtca cgctgatggc
    55381 cgccgggttg ccgctcaccc tgaaggacgc cgccccatga gccggtggct gctggccctg
    55441 ctggcctggg tctgggtcgg cctggtgtcg gcccaggagc tgcgcgacga cgcaggccag
    55501 ccgctgcgcc tggacgccgc gcccaggcgc atcgtcagcc tgctgccttc gctgaccgaa
    55561 accgtgtgcg cgctgggcgc ctgcgagcgc ctggtcggcg tcgaccgcta ctccaactgg
    55621 ccagcgagcg tgcagaccct gccccaggtc gggggcgggc tggacccgaa catcgaggcg
    55681 gtggtggcgc tcaaacccga cctggtgctg atcggcacct cctcgcgcgc ggcgctgcgg
    55741 ctgcgcgccc tgggcctgaa cgtgctggcg ctggagccgc gcacgcgcgc cgagctgcgc
    55801 cgcgtcacgc agcggctggg taccgcgctg cgcctggacg gcgccgacgc cctggcgcgg
    55861 cgcatcgacg acggcgtggc ggccgctgcg gcggcgctgc cggtggcggc gcgcggccag
    55921 cgcgtctact acgaaatcac cccggcgccg cacgccgccg ggcagggcag cttcatccac
    55981 gagctgctgc tggcgctggg cctggtcaac gtcatcgacg atccggcgct ggggccgttc
    56041 ccgcgcctca accccgagct ggtggtgcgc gccgaccccg acctcatcat ggtcagccag
    56101 ggcggggcgg ccgacatggc gcgccgcccc ggctgggcag gcctgcgcgc gctgcgggaa
    56161 ggccgggtgt gcgaattcaa cgccgcccag cgcgacatcc tggtgcgccc cggcccgcgc
    56221 atgcccgagg cggcacgcct gatggcggac tgcgcggcgc gccgcccact tcaaaaccga
    56281 tagctggccg cgccgaacgg aacttggctg acggcccgat gaacctgcac actcgcgcca
    56341 tggacgcgcc cgcccacgcc gccaccgccg ctctgccacc gcagggcggg gcgcgcgtgc
    56401 gctggctggc gctggccctg ctggcgctgg cgctgctcgg tctggggctg ggggccagtg
    56461 tcggcagcac cggtttcgac agcctgctgc gcgcctggca cgacgccgtc gcctggcaga
    56521 tcgtctggga catccgcctg ccgcgcaccc tgggcgcctg gctggccggc gcgctgctgg
    56581 ggctggccgg cgcggtggcg caggggctgt ttcgcaaccc gctggccgac ccgttcctgc
    56641 ttggcagcgc ctcgggcgcg gcgctggcgg tggcgctgac ctttgccgcg ctgggcgccg
    56701 gcggcctggt ggcgggcggc cacggcgtgg cgctgctgca ggcggcctcg ccctggctgg
    56761 cgcgcctggg cttcaccggc gccgccttcg tcggcgcgct cggcggcgtg ctgctggcgc
    56821 tggtgctggc gcagggcgtg cagcagacgc tgcgcctgct gctggctggc gtggtggtgg
    56881 gctacgtgct gggcgccgtg cgcgagctgg tgcaactggc ccagcccgac gtgctgcagg
    56941 ccatgcaggg cttcagcctg ggtgtcaccg gcttcgtcgg ctggggcgcc tgcgccgtca
    57001 tggccgccgt gctggcgccg ctgctgccgc tggcctgggc gctggcgcgc gggctcgacg
    57061 cgctcagcct gggcgaagcc acggcgcgca gcctgggcct gccgctgggc ctgctgcgcg
    57121 cgctgctgat cggcgtgctg gcgctggcca ccggcaccgc ggtggcgcag accggcctga
    57181 tcggcttcgt cggcctggtg gcgccgcacc tggcgcgcgc gctggcgcgc tgcacgcacg
    57241 ggccgttgct gctgctggcc agcctgatgg gcggcctgct gctgaccgcg gccgacatcg
    57301 cggcgcgcct gctgatcgcg ccgcaggagc tgccggtggg cgtgctcacg gccgtgctgg
    57361 gcgggcttta cctgctgtgg ctgatgcatc gccgcgggcg cgccgggggg gcgttgcagt
    57421 gacccaccgt gctacgcaaa ccatagctgt cggcgcacgt ctggcaagcg ccagcatcgg
    57481 aaaacaccag atactgcacg acatggcgct ggagctgccg cagggccgct ggtgcgccgt
    57541 cgtcgggccc aacggcgccg gcaaatcgac cctgctgcgc gtgctggcgg ggctgctgcc
    57601 gtgccagggc cagatcagcc tgtggggccg cccgctgcac gactggccgg cgcgcgcgcg
    57661 cgcccgccag ctggcctggc tgggccagca ggaaggcggc ggcgaagacc tgctggcctg
    57721 ggacatcgcc atgctcgggc ggctgccgca tcgcccctgg ctggccgatg ccagcgcgca
    57781 cgaccaggcc atcgtcgccg acgccctgcg ctccatgcag gtctgggcgc tgcgcgggcg
    57841 gcggctgggc gagctgtcgg gtggcgagcg ccagcgcgtg ctgctggcgc gcctgctggc
    57901 ggtgcaggcg ccgctgctgc tgatggacga gccgctggcg cacctggacc cgccgcacca
    57961 ggccgactgg ctggccgtgg tgcgcgccca ggtggccgcc ggcggcaccg tggtcagcgt
    58021 gctgcacgag ctgaacatgg cgctggcggc cgacgacatc gtggtgctgc gcgccggtcg
    58081 cctgctgcac cacggccccg ccgcctgccc ccacacccac gccgcgctgc gggcggcgtt
    58141 cgacggccgc atcagcattc gccagctcga acgccaatgg gtggcgctgc cccaacccga
    58201 tcgaagcgag cctgaccatg cagattgaat ctccccccac cgaacaacgc tacgagcgcc
    58261 ccgcaggtga acgccgcggt ctggtcatcg tgcacaccgg cgacggcaag ggcaaaagca
    58321 cggccgcctt cggcctggcg ctgcgcgcgt tcggccgcca gcatgtgcac ggcaagcagg
    58381 tggtcatcta tcagttcatg aaggtgccga ccgcgcgctt tggcgaacac cgggcgttcg
    58441 agcaactcgg cctgccggtg cacggcctgg gcgacggctt ttcatggaag agccaggacc
    58501 tggagcactc ggcccagctg gcgcgcgaag gctggcagaa ggcgcgggca gcgatcctgg
    58561 acggccagca ctttctggtg gtgctggacg aaatcaccta cccgctgatc tacggctggc
    58621 tgccgctggg cgacgtgctg cacaccctgc gccagcgccc gcgcgaggtg cacgtgtgcc
    58681 tcacgggccg gcgctgcccg cccgagctga tcgagctggc cgacacggtg acggagatga
    58741 cgctggtcaa gcacgccttc aaggccggca ttccggcgca gcgcggcatc gaggactgac
    58801 ggtccgccgc tccgcgcgcc gccggaaagc gtcggacgcc cgcacccgga cccctggcga
    58861 taggatgagc gcaccatgcc cctgacttcg cacacctatg ccacgccgct gggccagatg
    58921 ctggccctgc tgtccgagcg cggcctgtgc ctgcttgaat tcagccagca cacgcgtggc
    58981 ctggggcgcg agatcgccca ggtcgaagcc gcccgcggcg gccccgcgca ggccggcgcc
    59041 catgccctgg gcgagcagct cggccgcgag ctggccgaat actttgccgg ccggcgccgc
    59101 cacttcggca tcccgctcga cctggtgggc acgccgttcc agcaaagcgt gtggcaggcg
    59161 ctgctgcaga tcccctatgg ccagacgcgc agctacgcgc aacaggcgca gtccatcggt
    59221 cgcccgaccg ccacgcgcgc cgtggccgcc gccaacggca gcaacaaaat tgccctgatc
    59281 gtgccctgcc accgcgtcat cggcagcgac ggcagcctga ccggctacgg cggcggcatg
    59341 ccgcgcaagc agtggctgct ggcgctggag cgcagcggcg tgccgccggg cggcggcatg
    59401 gatctgacct gactcagcgt tcgcgatggc tttgcagcgg cgctgcgcaa caccttaggc
    59461 gcgagcagcg ctgcgccagc cgccccacgc cgcaggcgcg ggtaggtgct cgattcgaca
    59521 tgcaccggcg cgcacaaact gatggcgctg gcttggggct ggaggtgacc gcgatggtgg
    59581 cacgactgct gggagcggct ctccgatcgg ttgggtgcgg gtcggcacgg ctgcgtcggt
    59641 ctgctggcgt gtgcaccgac gggttaaagt gcggacacgg cgttacaatt ttcgcgtttt
    59701 tcgtccatac atatatctaa ggagatctgc atgaatgcaa ggaatttcat tcgccggggc
    59761 atcactgccc tggccatggt gctgggtgcg gccagccagg cccaggctgc caacgtggcg
    59821 ttctggagtg actactactc ggctgccagt ttcacggcat tgaatgccac gcacacggtc
    59881 acccaagtca cggtggccaa catcgacgcg ggtgcgctgt cggcctacga cgtgctggtg
    59941 ctggggcact tgaatcctag catccctccc acggccacca cgtgcagcca gattcagagc
    60001 ttcctggccg
//
LOCUS       DAOQTZ010000004        59276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_579_length_59276_cov_13.570828, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000004 DAOQTZ010000000
VERSION     DAOQTZ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 59276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59276
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_579_length_59276_cov_13.570828"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKN51_00970"
     CDS             <1..198
                     /locus_tag="PKN51_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83697.1"
                     /translation="PQNQGAWFFIQHNIHENMHEGQKLGYAGRAASASPAVGYAHLHQ
                     DQQKALIDAAFGRLKGFVLQK"
     gene            300..1568
                     /gene="odhB"
                     /locus_tag="PKN51_00975"
     CDS             300..1568
                     /gene="odhB"
                     /locus_tag="PKN51_00975"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNT83698.1"
                     /translation="MAIVEVKVPQLSESVAEATLLQWKKKAGEAVQVDEILIEVETDK
                     VVLEVPAPASGVLTEIVQADGATVVADQLLARIDTAAVAGAAAPAPAAAPSAAPAPAA
                     APAADKSGVAMPAAAKLMADHQLPAGSVAGTGKDGRVTKGDVLGAIQSGAAGAGGASS
                     GAQKAAQIPTGVPTTALPQVAAPAAPQLGDRPEQRVPMTRLRARVAERLLQSQASNAI
                     LTTFNEVNMQPVMDMRKRFQDKFEKEHGVKIGFMSFFVKAAVHALKKYPVLNASVDGN
                     DIVYHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKKIAEFGAKARDGKLGIEEMT
                     GGTFSISNGGVFGSMMSTPIINPPQSAILGVHATKDRAVVENGQVVVRPMNYLAMSYD
                     HRIIDGREAVLGLVAMKEALEDPARLLFDI"
     gene            1639..3069
                     /gene="lpdA"
                     /locus_tag="PKN51_00980"
     CDS             1639..3069
                     /gene="lpdA"
                     /locus_tag="PKN51_00980"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNT83699.1"
                     /translation="MSNQQFDVIVIGAGPGGYIAAIRAAQLGQKVACIDEWKNDQGGP
                     APGGTCTNVGCIPSKALLQSSEHFEHANKHFAEHGISTGKVTMDVAKMVGRKDAVVKQ
                     NNDGILYLFKKNKVSFFHGRGSFVRAVDGGYEIAVKGAKDETLVGRQIIVATGSSARA
                     LPGVPFDEEQVLSNDGALRIAAVPKRLGLIGSGVIGLEMGSVWRRVGAEVTVLEALPT
                     FLGAVDEQIAKEARKAFDKQGLKIELGVKVGEVKVGKKGVSVAWTDAKGAAQELVVDK
                     LIVSIGRVPNTTGLNAEAVGLRLDERGAVVVDDDCRTNLPGVWAVGDVVRGPMLAHKA
                     EEEGVAVAERIAGQHGHVDFNLIPWVIYTSPEIAWVGKTEQQLKAEGAKYKAGSFPFM
                     ANGRARALGDTTGLVKFLADAATDEILGVHIVGPMASELIAEACVAMAFRASSEDIAR
                     ICHAHPSLGESMKEAALAVDKRTLNF"
     gene            3143..4243
                     /gene="zapE"
                     /locus_tag="PKN51_00985"
     CDS             3143..4243
                     /gene="zapE"
                     /locus_tag="PKN51_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNT83700.1"
                     /translation="MSDVRQAYELELKARGFQSDPAQLRAVQALQRCADDWAAYKSSR
                     SNRLKKLFNRPEIPRGVYMYGGVGRGKSFLMDCFFNAVPIRRKTRLHFHEFMREVHRE
                     LAALQGTVNPLDELGRRMAKRFKLICFDEFHVADITDAMILHRLLVALFDNGVGFVTT
                     SNFKPDDLYPGGLHRDRILPAIELLNQRMEVVNVDNGTDYRHRTLQQVQLYHTPLGAA
                     ADAAMQAAFDQLASRADEDPVLHIEAREILARRRAGGVVWFDFRALCGGPRSQNDYLE
                     IASQFHTVLLSGVPHMPVRMASEARRFTWLVDVLYDRRVKFILSAEVPPEQLYTEGPL
                     AHEFPRTVSRLNEMQSAEYLALEHRTVDTQLT"
     gene            4240..4635
                     /locus_tag="PKN51_00990"
     CDS             4240..4635
                     /locus_tag="PKN51_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83701.1"
                     /translation="MSRLPISLLLCTLLAACASGSLHDQHLARRAADAADAQALAATA
                     PPAGQPLAPWLQAQRARIVSERGAATRHFEEAEKACWQRFAVNDCLQQARVQRRTALD
                     RLRQQELAINDLERQRQADARRLEPRNGS"
     gene            complement(5173..5973)
                     /locus_tag="PKN51_00995"
     CDS             complement(5173..5973)
                     /locus_tag="PKN51_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNT83702.1"
                     /translation="MEKGYRLSAATGLHKGDRDYQQDQVALFAHARANGCVMGVVADG
                     MGGRTGGRKAADQVMLTARQLFERYAPDSEDATAVLRQIGEEAHLIIKLTAIAAEQEP
                     HSTMAAFIINVGGECHWAHTGDSRLYHFRGARLVRRTKDQSYVQALVDQGELTEDEAA
                     VHPKSNVLLGCLGTEEAPKFDVHYIPQLQIGDALLACTDGLWHYFTPEELGSVLHSLP
                     PREASEFLIDKARTRAMGGGDNLALAIVKVEPLQTNQPVVGSAFSPLR"
     gene            6097..6300
                     /locus_tag="PKN51_01000"
     CDS             6097..6300
                     /locus_tag="PKN51_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNT83703.1"
                     /translation="MTANLHSPERRLIELRMEHADLDASIDRLAGQAPLDELMLQRLK
                     KRRLALRDAIARLQAQLEPDEPA"
     gene            6287..8368
                     /locus_tag="PKN51_01005"
     CDS             6287..8368
                     /locus_tag="PKN51_01005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNT83704.1"
                     /translation="MNRPERVSPVATPGAPHGPEPGALAQAVGAAFDDHGPLSQAAEG
                     FRLRDGQTRLALALARVIEQGGVLVAEAATGVGKTFAYLVPALLSGERVLLSTATKTL
                     QDQLFGRDLPRLARALGLPVSMALLKGRASYLCLHRLEMAHQTGVSSAPGVRQQLSKI
                     ERWSKITRSGDLAELPDLDERSPVIPLVTSTRENCLGADCPQFRACHVQLARRQAMAA
                     DVVVINHHLFFADLAIRESGMAELLPSVRVAVFDEAHQLNETGIQFLGQQLASGQLLD
                     FARDLLAAGLRQARGLCDWQALASAVEVATRELRLAAGAGRAAGRLRWLEAVPEGVDA
                     AAWPPALRQLHQALADAAAALATVSELAPDFVRLHQRALLLAQRAERFGQPCEPALVR
                     WVDVGASLRLVESPLDIADTVRTRLLAGPQDDGRARAWAFVSATLGDDERLSWFTEPC
                     GLQDAEVLRIGSPFDYARQAALYVPTHIARPADPAHSADVARLAGDAAARLGGRTMVL
                     TTTLRALRRIGELLGERFGAGADIEVLMQGSLPKRVLVERFRAGGSAGRPGCVLVASA
                     SFWEGVDLPGQALQLVVIDKLPFPPPGDPLVEARCRRLQAQGRSAFADHSVPEAAVAL
                     KQGAGRLIRRESDRGVLVVCDTRLVQMGYGRRLLAALPPMRRLPSPQAFEAALDELLA
                     EDASKTIAAGA"
     gene            complement(8430..9233)
                     /locus_tag="PKN51_01010"
     CDS             complement(8430..9233)
                     /locus_tag="PKN51_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNT83705.1"
                     /translation="MQGARLSVAGAVAAALAVLLAACSSKPIDRTAGWSPNRIHAEAK
                     DELNAGAYDKAIPLYETLEGRAAGTTLAQQAQLDKAYALFKDGQKAEAIATLDRFMRL
                     HPASPAIDYALFLKGVINFNDELGMLSFLARQDLSERDQKAAKEAFESFNELVTRFPD
                     SRYAPDARARMRYTINALAQYEVHVARYYFTRGAYVAAINRAQQALADYRDAPSLEEA
                     LYILTQSYDRLGMTELRDDARRVLDRNYPNSTYLTHGLRGADKPWWQLW"
     gene            9332..10315
                     /locus_tag="PKN51_01015"
     CDS             9332..10315
                     /locus_tag="PKN51_01015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT83706.1"
                     /translation="MEDAGAAAELRQLALPAELHGQRLDRALAALVPEFSRNYLRQLI
                     EAGVVHMQGQPVVKPAQKVRAGDHLAIELRPTPMSQAFVPQAMALDVVYEDQHLAVID
                     KPAGLVVHPAPGHWSGTLLNGLLARDAAAAHLPRAGIVHRLDKDTSGLLVVARSRAAM
                     DALVALIAARQVRREYLALAHRPWSGAPAREIDAAIGRDPRQRLRMAVLDPARHPARP
                     AQTRFELLQNAPQGCLLRCLLRTGRTHQIRVHLAHLGHPLVADALYGGAPAAGLTRQA
                     LHARRLAFRHPLTGQTLDFVAPLPTDLTHALSAWGLTMPADDMDPAASPAR"
     gene            10417..11214
                     /gene="scpB"
                     /locus_tag="PKN51_01020"
     CDS             10417..11214
                     /gene="scpB"
                     /locus_tag="PKN51_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNT83707.1"
                     /translation="MNSTDAKRILETALICAPQPMPLRELRVLFDDELGADTLKNLLL
                     ELQDDWAQRGVELVQVASGWRFQSRPEMREYLDRLHPEKPPRYTRATLETLAIIAYRQ
                     PVTRGDIEDIRGVTVNTQLIKQLEDRGWVEVIGHRETVGRPGLYATTRQFLDDLGLES
                     LDQLPMLDTPSEQAASMFDALGQAAEDAPAAAATDAEPAATPDVEAGEPAAAPAAKAE
                     PESPSAPDADDSAAPAATPASAPEPEPVDDSPPSRPDTAEHEAPHVP"
     gene            11204..12565
                     /locus_tag="PKN51_01025"
     CDS             11204..12565
                     /locus_tag="PKN51_01025"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT83708.1"
                     /translation="MCPDEPTAPAADDQASATPGPARRSRTKPTSEPAQTAPNATDMV
                     AIEADAEPTGAPTAADQAPVVIDDVLSGRFDAEADGGASDVPPKRVLLPQPEQPKLHK
                     VLAQAGLGSRLEMEQLILQGRISVNNQPAHIGQRIQFGDAVKINGKPIKLRIAPPPAR
                     VLAYHKPVGEVVTHDDPQNRPTVFRKLPRLPQGKWQSVGRLDLNTEGLLLLTNSGELA
                     NRLMHPRFGLEREYAVRVLGALSDEEKQQLLDGVPLDDGMAQFSSIQEEGQGEGANQW
                     YRVTISEGRNREVRRMIESVGHAVSRLIRIRYGAMMLPRGLRRGQWMELGERDIRALG
                     AASGTPEHVLRGGREPGPRPSEPPRRGRGDRGRGSGPRPNMAGGGRPPANTGSGKPRR
                     RDGGPAGGAGAPQPDPMKTSFGYIGADSLSRQRQSAGRGAPGARGGGGRARRGPAPGG
                     RRG"
     gene            12688..13113
                     /gene="ndk"
                     /locus_tag="PKN51_01030"
     CDS             12688..13113
                     /gene="ndk"
                     /locus_tag="PKN51_01030"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNT83709.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYARFEAAGLKIVAARMQHLSR
                     REAEQFYAVHKERPFFKDLVEFMISGPVMIQVLEGEGAIAKNRDLMGATDPKKADKGT
                     IRADFADSIDANAVHGSDAPETAAVEVAFFFSGMGVYSR"
     gene            13141..14307
                     /gene="rlmN"
                     /locus_tag="PKN51_01035"
     CDS             13141..14307
                     /gene="rlmN"
                     /locus_tag="PKN51_01035"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNT83710.1"
                     /translation="MTTPVNLLDFDLEGLAAWCDTLGEKRYRATQLFRWIHQRGVAEF
                     GQMSDLARSLQGKLHGRALVAAPAVIRQHASKDGTIKWLFDVGGGNAVEAVYIPEDDR
                     GTLCVSSQAGCAVACRFCSTGHQGFSRNLSTAEIVGQLWFAEHFLRRHLKTDERVISN
                     VVMMGMGEPLQNYDRLLPALRTMLHDHGYGLSRRRVTVSTSGVVPMIERLARDCPVAL
                     AVSLHAPTDALRDHLVPLNRKYPIDELLRTCTAYLAHAPRDFITFEYCMLDGVNDSAE
                     HAQALVELMRRHAASGLRGKFNLIPFNPFPQSGLTRSPMARVQAFGKLLNDAGIVTTI
                     RKTRGDDIDAACGQLAGDVQDRTRVGVRIARQRTVALHQLAPAGAAPAPRKVSS"
     gene            14304..15134
                     /gene="pilW"
                     /locus_tag="PKN51_01040"
     CDS             14304..15134
                     /gene="pilW"
                     /locus_tag="PKN51_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNT83711.1"
                     /translation="MSLHACAPSAASRRRLLALASFGLAAWLLAACAAPPGLGDNPQE
                     LPTASDETDARRRARTRLALATGYYENGQHTVALDEQKKALQADPNFGDAYNLGGLIY
                     MALGDQALAQSHFQRAIALNPRDGDALHNLGWLQCQQGQLAAANQSFERALAVPTYPG
                     RARTLMAQGVCEARAGNLARAEAALMRSYELDAGNPVTAFNLSQLLYQRGDYTRAQFY
                     VRRLNNSELANAESLWLGIRVERRLNNRQAMEQLASQLRRRFPQSRELLAYERGAFDD
                     "
     gene            15127..16008
                     /locus_tag="PKN51_01045"
     CDS             15127..16008
                     /locus_tag="PKN51_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT83712.1"
                     /translation="MTEAHTGPASLHDTPAHGAGPRSAGQMLRALRESAGVDPALLAS
                     AMKVSPQKLDALENDRLDELPDVTFARALASAICRAFGTDPAPVLERMPVSAPGLRPV
                     AQAPINEPFRRSSDGPATVLAGGLSKPLAIGVIALLLGAAALWLLPTLPIQLNAPAPA
                     SEPAATDGVVRESVMPPAATSAEPPAPPAPAELSASAAAAEQAPPPAAAAPAAASTDL
                     LYFSAQAETWVTVRDASGKNLINRALNAGDSVGVSGEPPLAVTIGRKDAVEVRVRGEP
                     FDHRSISKTPVSRFEVR"
     gene            16011..17279
                     /gene="ispG"
                     /locus_tag="PKN51_01050"
                     /gene_synonym="gcpE"
     CDS             16011..17279
                     /gene="ispG"
                     /locus_tag="PKN51_01050"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNT83713.1"
                     /translation="MPSPNCTPIEVARPAPRRTRQARVVWGRRVVTVGGDAPVRVQSM
                     TNTDTEDAIATAIQVKELALAGSELVRITVNTPEAAAQVPYIREQLDRMGIDVPLIGD
                     FHYNGHRLLTEHPDCAQALSKYRINPGNVGKGAKRDTQFGQMIEAALKWDKPVRIGVN
                     WGSLDQDLLAGLMDENSRRAQPWDARQVMYEALISSALGSASQAVELGMDPAQVILSC
                     KVSGVQDLIAVYRELASRGDHPLHLGLTEAGMATRGTVASSVALAILLQEGIGDTIRV
                     SLTPQPGEARTQEVVVASEILQALGLRAFVPSVTACPGCGRTTSTVFQELAKQIDDHL
                     RAQMPLWRSQYPGVEKLKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVYIDGEK
                     AMTLRGEHIAAEFQQIVARYIEQRFGRRQP"
     gene            17356..18663
                     /gene="hisS"
                     /locus_tag="PKN51_01055"
     CDS             17356..18663
                     /gene="hisS"
                     /locus_tag="PKN51_01055"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNT83714.1"
                     /translation="MSGSKQITAIKGMNDILPPASARWDWLEDVVRRVMAQHAYRNVR
                     TPIMELTQLYTRGLGEVTDIVEKEMYAFEDRADQHGRADHLALRPEGTAGLVRAVAEH
                     NLLYDGGKRLFYMGPMFRRERPQRGRYRQFHQIGAEALGFGGPEVDAELILLAVALWR
                     ELGLTGWRLELNSLGQPDERREHRAALVAYLQAHHDQLDEDARRRLHSNPLRILDTKN
                     PAMQALVEGAPKLLDYLGEASRRHFAAVQAILAAAGVSWHINPRLVRGLDYYNLTVFE
                     FVTDRLGAQGTICGGGRYDYLIEQIGGKPAPAVGWALGVERVLELLQEEGVHTLEPVP
                     DAYAIIPDAAALPQAMRCLQQLRALGVAVQMHAGSGEGMGSMKSQFKRADASGARHAL
                     IFGADELAAGEVTVKALRDGAGAQLRQPLAQPDAWADILKSTA"
     gene            18681..19358
                     /locus_tag="PKN51_01060"
     CDS             18681..19358
                     /locus_tag="PKN51_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT83715.1"
                     /translation="MATHLDLEEQEQLDALKHFWNRWGNLITWVLIAVLGAYAAWNGW
                     QYWERRQAGLAAVLFDEVERAAQAGDAARVERALADIKDKFGGTTYAAHAGLRGARFF
                     DEQGKTAETKATLAWVAEKAPNDGLRAVARLRLASVQLGEKAYDEALRTLEAGFPPSF
                     VALAADRRGDVLLQQGKRELAAAEFGKAYQGMAADTAGDYRRLVGIKLNALGIDPDAG
                     AKAGAAS"
     gene            19355..20494
                     /gene="bamB"
                     /locus_tag="PKN51_01065"
     CDS             19355..20494
                     /gene="bamB"
                     /locus_tag="PKN51_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNT83716.1"
                     /translation="MTARGTVRRRIACGASLLAALVLTACAGGSPRPQPAELPANIAL
                     IGVRQAWTARLPAIDFPLQVAVQGQQVTLAANDGSVVTLDAASGRELGRASAGAPLTA
                     GVGSDGRVSAVVTRDNRVVALEGGRELWRQKLGTQSYTAPLVAGGRVFVLGADRSLSA
                     FDGRNGARLWHLERPAEPLVLRQAGVLQAVGNTLVAGLAGRLVGVDPDSGSVRWEAPI
                     ASARGTNDVERLIDLVGGVSRHEGVVCARAFQTAVGCVDTASGRLRWSKPASGATGVA
                     GDGQLLFGAEADGRVLAWRADTGDRAWVNESLQWRRLTAPLVLGRSVVVGDGNGLLHF
                     LSRQDGTPLDRLSTDGTGIAATPVAAGNTLVVATRGGTVYGFVPE"
     gene            20630..21976
                     /gene="der"
                     /locus_tag="PKN51_01070"
     CDS             20630..21976
                     /gene="der"
                     /locus_tag="PKN51_01070"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNT83717.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTRSRDAIVADFAGLTRDRHYGQ
                     GHLGKREYIVIDTGGFEPRAESGIYKEMAKQTRQAVAEADVVLFLVDARAGLSAQDHD
                     IADYLRRLGKPCLLVANKAEGMREGAQLAEFYELGLGAVIPVSAAHGQGVRSLLEGAL
                     DALDLPDVDEAPPDAADGVIRLAVAGRPNVGKSTLINTWLGEERLVAFDLPGTTRDAI
                     SVPFERAGRQFELIDTAGLRRKGKVFEAIEKFSVVKTLQAIESAHVVLLLLDATQGVT
                     DQDAHIAGFILEAGRAVVLAINKWDAVDDYQRELVRRSVESRLGFLKFAELHFISVRK
                     RQGLGPLWASIARAHQAAMRKLPTPQLTRTLLEAVQFQAPKRAGMYRPKLRYAHQGGM
                     NPPVIVVHGNSLEGVTDTYKRYLEGRFRQVFDLIGTPLRIEMKTTHNPYATRPDQG"
     gene            22123..22368
                     /gene="hfq"
                     /locus_tag="PKN51_01075"
     CDS             22123..22368
                     /gene="hfq"
                     /locus_tag="PKN51_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNT83718.1"
                     /translation="MSNKGQLLQDPFLNTLRREHIPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNNVTQMVYKHAISTIVPGRPVNFSATGEVAESD"
     gene            22455..23651
                     /gene="hflX"
                     /locus_tag="PKN51_01080"
     CDS             22455..23651
                     /gene="hflX"
                     /locus_tag="PKN51_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNT83719.1"
                     /translation="MPRTATPERAAAILVGVDLGVPHFDGQLQELGLLAETAGLQPVA
                     RLTCKRQAPDAALFVGRGKVEEIRLLAQSEGAREVLFDQALSPVQQRNLERAIGLPVN
                     DRTLLILEIFAQRARSHEGKLQVELARLQYLSTRLVRRWSHLERQQGGIGGRGGPGET
                     QIELDRRMIGEAIKRTKDRLQKVKRQRQTQRRQRERRHTFNISLVGYTNAGKSTLFNA
                     LVKARAYAADQLFATLDTTTRQLYLGQAGRSVSLSDTVGFIRDLPHGLIEAFEATLQE
                     AADADLLLHVVDAANPGHLEQIEEVQRVLREIGAAELPQLLVFNKLDELEPARRPLQL
                     KDSVTLDGRELTRIFVSARSGEGLPLLRAELEAAVQSQALEAGGDGVPAPIGHNDGLH
                     SREVDA"
     gene            23648..24994
                     /gene="hflK"
                     /locus_tag="PKN51_01085"
     CDS             23648..24994
                     /gene="hflK"
                     /locus_tag="PKN51_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNT83720.1"
                     /translation="MNDRTSAPRPASWPGRIRGMFNLHDPRWGRDDEPSGQEPGQRPD
                     EPQRPPPRGQGPQAGPPDLDELWRDFNRKLGGLFGGGRGRGRNPYSGGGMPPEMKGRG
                     AGIGIGVIAAVALAIWLASGVFIVREGEQAVVTTFGRFDGIRKAGLNWRMPYPIQRHE
                     TVRVTQIRSVDVGRDSIIRSTGLRESAMLTQDENIVEIKFAVQYRLNDARAYLFESKS
                     PDDAVVQAAESAVREVVGKMTMDTAMAEERDQIAPRVRDLMQAILDRYQVGIEVVAVN
                     MQQGGVRPPEQVQAAFDDVLKAGQERERVKNEAQAYANDVVPRAVGTAARLKQEAEGY
                     KARIVAQAQGDAQRFSSVLSEYQKAPQVTRERMYLDAMQQIYGGVNKIIIDSRAGGNL
                     MYLPLDKLLQGAAGAGAAAGAAPAAAQAPAPATAPAAPAADPRARDNARTRERETR"
     gene            25010..25897
                     /locus_tag="PKN51_01090"
     CDS             25010..25897
                     /locus_tag="PKN51_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNT83721.1"
                     /translation="MNRVGFIGTTVLILLALLGSTVFVVDQRQFGVVYQLGQIQRVIT
                     QPGLNFKLPPPMQNVTYIDKRLLTLDSMDTEPMLTAEKQRVVIDWYVRWRITDPQAYI
                     RNVGLDESAGALQLNRVVRNAFQEEINRRTVRELISTKRETLMADVKREVSDAVKGAK
                     PWGIDIIDVRITRADYVESITESVYRRMQAERQRVANELRSTGAAEGEQIRANADRQR
                     EVTVADAYREAEKVRGEGDAQATRIYGDAFGRDPQFAEFYRSLDAYKASIGKKGDVLV
                     IDPASSDFFRNLRGAPARR"
     gene            25915..26100
                     /locus_tag="PKN51_01095"
     CDS             25915..26100
                     /locus_tag="PKN51_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNT83722.1"
                     /translation="MDGATWLAALGLALVLEGLVPFLAPRAWRQAFSQLLQLRDGQVR
                     FFGLLALAGGLALLWLA"
     gene            26170..27333
                     /locus_tag="PKN51_01100"
     CDS             26170..27333
                     /locus_tag="PKN51_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNT83723.1"
                     /translation="MSAWLLPDHIADVLPSEARHIEELRRELLDTARGYGYELVIPPL
                     IEHLESLLTGTGEALGLQTFKLVDQLSGRSLGVRADTTPQVARIDAHLLGRQAVTRLC
                     YCGPVLHTQPERPHATREPLQLGAEIYGHAGREADLEILSLALDCLRAAPVHGLSVDL
                     ADARIVRALLAGVLVDPERLTEVHLALAAKDASELARLTAAFPASARQGLCALVGLYG
                     DAGVLDEAEKALSGAPGVREVLSNLKWLASQLEGVRVSFDLADARGWGYYTGARFAVY
                     AEGAGDALLRGGRYDGVGAVFGHKLDRERPAVGFSLDLKQLVGVAAPRPLRAAIRAPW
                     GDAPGLRKAVAGLRRRGETVVCALPGHADQIDEFHCDRELIEAGGQWVIQALG"
     gene            27443..28822
                     /locus_tag="PKN51_01105"
     CDS             27443..28822
                     /locus_tag="PKN51_01105"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNT83724.1"
                     /translation="MKPSTTGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHN
                     AGHTLVINGVKTALHLIPSGIMRPGVTCYIGNGVVLSVAKLLEEIAGLERAGVQVRER
                     LRISEGCPLILPLHAAMDVAREAAREQGGSEKIGTTGRGIGPAYEDKIARRALRAQDL
                     KHPERFAVKLRELLALYNHELLGYLGSARIAFNEALTPYLKDGALQFEPIYDEAMRQA
                     EQVRPMLADVSRELNEAHQQGANLLFEGAQGTLLDVDHGTYPFVTSSNTVAGNAAAGA
                     GVGPGLLHYVLGITKAYCTRVGGGPFPTELDWQTPGTPGWHMSTVGAEKGVTTGRSRR
                     CGWFDAALLKRSAQVNGLSGLCITKLDVLDGLPELQLCTGYELDGETIDILPLGADDI
                     ARCKPIYETMPGWSESTVGATRYEQLPIHARLYLQRIEHVTGVPIHVVSTSPDRDHTI
                     MLRHPFLPT"
     gene            28892..29416
                     /locus_tag="PKN51_01110"
     CDS             28892..29416
                     /locus_tag="PKN51_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNT83725.1"
                     /translation="MLTEDGKHLYVSYDEYHALIEKLALKVHQSGWRFDTILCLARGG
                     MRPGDILSRIFDVPLAIMSTSSYRAEAGKVQGHLDIAHYITTPQGQIAGRVLLVDDLA
                     DTGVTLQAVVERLRNDYPPITELRTAVIWTKGSSSFQADYSVEHLPTNPWIHQPFESY
                     DGLTPDKLLAKWKV"
     gene            29526..30743
                     /locus_tag="PKN51_01115"
     CDS             29526..30743
                     /locus_tag="PKN51_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNT83726.1"
                     /translation="MTDPITQLIHHPYQPPAGFAAPQPAVHKASTVFFPNVAAMRERD
                     WKHKSGYTYGLHGTPTTFLLEERIATLEGGLQCVLVPSGLAAIANVALALLQTGDEVL
                     IPDNAYGPNKALAEGELAAWGITHQYYDPMDLADLAARIGERTRLVWLEAAGSLTLEF
                     PDLIGQVRLCRARGVPCALDNTWGAGLAFNPFDLDGQGLGVDLSAHALTKYPSGGGDV
                     LMGSVTTRDEALHLKLKLTHMRLGLGVAANDAEAVLRALPSVALRYRAQDQAARALAG
                     WCAQQSAFAQVLHPALADAPGHAHWQQVCGAADARGQGAAAGLFSAIVHERYSQAQVD
                     AFCDTLKRFKLGYSWGGPISLVVPYDLAALRARGWPAHLRRGTLVRFSTGLEALADLQ
                     ADLQQALNRALPS"
     gene            30913..>31691
                     /locus_tag="PKN51_01120"
     CDS             30913..>31691
                     /locus_tag="PKN51_01120"
                     /inference="COORDINATES: protein motif:HMM:NF021472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HNT83727.1"
                     /translation="MSQPDTDPTAAPPAAAPAPAYQPYVPPPVTTPQAIMVPLGLADP
                     LRWLGRGWRDFMAAPGIGLFYGVCFWLMAALLALVFRSKPEYVMSIASGCLLVGPFLA
                     MGLYEVSRRREQGLVPELAASLTCWDRHVRSMGMLVLVLIVLELLWGRASLVVFAVFF
                     NTGMPSTTGVLQAIFNPDNLEFVLVYLTVGGFFALLVFSTTVVSIPMILDRDTDAISA
                     AITSIRVVFANPLTLLWWGLLITLLIALSLLSLGLGLLLVGP"
     assembly_gap    31692..31791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(31874..33052)
                     /gene="mnmH"
                     /locus_tag="PKN51_01125"
     CDS             complement(31874..33052)
                     /gene="mnmH"
                     /locus_tag="PKN51_01125"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNT83728.1"
                     /translation="MSVQTIAAADALARLAEFSAIIDARSESEFAHDHLPGAVNWPSL
                     NDQQRARVGTLYKQASPFEARKLGAALVARNIADHIERELAHTSRRWRPLLYCWRGGQ
                     RSGALALVLGQIGFQVTLIEGGYKAFRVALLTDIPAQVQRLRLRVVCGPTGSGKTRLL
                     HALAAAGAQVLDLEGLAQHRASVLGLIPGQPQPSQKHFEMRVWDALRHLEPARPVYVE
                     AESKKVGNVTVPEALIAAMRASDCLRIELADDERVALLLEDYPFFVRDPDFFCQRLDT
                     LVALRGRAVIDDWKARVAAGHTDQVVRELLARHYDPGYASSTQRNFARFGQALAVPLA
                     DRSPASLRAACALILASQAPATPPDGVQPGSGAGAGTGAGTQASAGRGGGSRHCTAPR
                     "
     gene            complement(33049..33681)
                     /locus_tag="PKN51_01130"
     CDS             complement(33049..33681)
                     /locus_tag="PKN51_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNT83729.1"
                     /translation="MNRRDCIRFLLAAAPAGLAAQTAAPPLILVLGDSISAEYGIARG
                     SGWVALLEQRLAREQIAARVLNASVSGDTTAGGRSRLPALLKAHRPAVVVIELGGNDA
                     LRGLSLEMTEANLTAMTQAAQAAGAKVLLAGMQMPPNYGADFGRRFFALYPQVAKAQR
                     AALVPFLLKGVADGDHAQALFQPDRIHPTAAAHPIILDNVWPELRRLLKP"
     gene            33697..34434
                     /locus_tag="PKN51_01135"
     CDS             33697..34434
                     /locus_tag="PKN51_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT83730.1"
                     /translation="MTDVSHGAPAGARRAPIIAVEHVFKSVTDSSGTLDILRDIHFSL
                     AERETVAIVGASGSGKSTLLSIIAGLDTPTRGTVRIAGDDLFAVSEDARAALRARRIG
                     FVFQSFQLLGNLTALENVMLPLELAGRRDARAAATEMLARVGLAERLRHYPKVLSGGE
                     QQRVALARAFVLHPAVLLADEPTGSLDHATGEKVMALMFELNREQGTTLVLVTHDRGI
                     AARCQRRLTIDAGCIAADEQAESLSIK"
     gene            complement(34476..35957)
                     /locus_tag="PKN51_01140"
     CDS             complement(34476..35957)
                     /locus_tag="PKN51_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNT83731.1"
                     /translation="MIRLPTLSPLEHAAFVAAGLLLYVLVTRARQQRRHPYAALAWVL
                     GIAAFPYLGVPLFLLFGTRKTVRPSTPRQPVPAGPWARLAPAWATGLLAALGVPEARP
                     QAALRFHADGAQALAELRALIASAERTLDVCTYVLGDDQVGAAVADELAARARAGVRV
                     RLLVDSVGSLRSRRSHDARLRAAGVRVRRFMPALRNPRRGRTNLRNHRKLVVADGLRL
                     WGGGRNLADEYFIGRAGEAPWIDLSFSAEGALATQAMALFEGDWRIALGLRRASGVPY
                     AERALRHEALLHAYQPADASAAALASAPPAASGAAPSAQVALAHWVPSGPDFHEDVLH
                     ALLVSAAFHAQQRLLLATPYFVPDEGLLEALLLAAKRGVRVTLLLPRRSNHRLADWAR
                     GRAVRALVEHGVEVRLLPTMLHAKAVVVDQALALCGSANLDSRSLFINYEAMAACYGR
                     AEIDWLAGWITRTAADGEPASARPPGLLRDVLEGVVGALAFQL"
     gene            36201..36935
                     /gene="rlmB"
                     /locus_tag="PKN51_01145"
     CDS             36201..36935
                     /gene="rlmB"
                     /locus_tag="PKN51_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNT83732.1"
                     /translation="MSTPKVLFGFHAVGVRLKTAPASVIEVYVDPGRRDARMKQFLAR
                     AAEAGVRVIEADGPRLARLCGGAGHQGVVARVQPIAQTHSLDELLDGVAEPPLLLVLD
                     GVTDPHNLGACLRVADGAGAHAVIAPKDHAAGINATVAKVASGAAETVPYLMVTNLAR
                     TLGELKERGIWVIGTSDDAPQTLYQADLKGPTALVLGAEGPGMRQLTRKSCDLLVRIP
                     MHGAVESLNVSVASAVCLYEARRQRG"
     gene            37388..38458
                     /locus_tag="PKN51_01150"
     CDS             37388..38458
                     /locus_tag="PKN51_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT83733.1"
                     /translation="MPSSLVARPAEPSRRLLLRLAAAGALGLAGAAAAQRPTPERTSV
                     TLAVGGKTSLPCLPLTIAEQLGYFRDEGLQVELQDHPGGGLAQQALLLGRADVAAGGF
                     EHPVLLRPRGTHCRAFVLLGRAPQLVFGVGTRALPEFRSLAQLRGRRVGVTSADSSSH
                     WFAQRLLARAGLAPTDVEYVGVGTAITPVAALREGRIDALADIDPAISLLEYRADIRV
                     VADTRSLRGTHELYGGPMPGACLYARDAFVLRYPQTVQALTNAVVRALKWLHTAGPSD
                     IVRTVPESYMHGDRSVYLSALDKAREALSPDGMASDDSVATAHRIVAQQLQATDGLRA
                     TAVGASHTNDFARRARQRFLVS"
     gene            complement(38490..40088)
                     /locus_tag="PKN51_01155"
     CDS             complement(38490..40088)
                     /locus_tag="PKN51_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNT83734.1"
                     /translation="MKWTLSLTLLAGLWLAGCAAPPGPATPERDVSTATPTRAEPRGA
                     QRMPDALPPVRITELRGNHVAALETPADLWERIRRGYAMPDLQDELVQRHEQWYSTRP
                     DYIERMVERSRLYLFHVVEELELRGMPTELALLPYIESAFNPQAVSSAKAAGMWQFMP
                     GTGRDFQLTQNILRDDRRDVLDSTRAALDYLQKLHDMFGDWHLALAAYNWGQGNVKRA
                     IERNQAAGLGTGYLDLKMPAETRNYVPKLQAVKNIIAQPQRHGAVLPLIENHPFFDTV
                     DIKHDIDVDVAARLAQVRLEDFKALNPSQRKPVIFAAGTPQVLLPWNNAALFQQALAK
                     ADPGTLSSWTAWVAPQNLPSREVASRFGMDESDLREINNLPHGVVIKSGSTVLVRRAN
                     GSATAVASHVVNNAQLAYAPEIVLRRTRVRARKGDTIASVAARYDLPAATVAGWNGTQ
                     AGARLKPGQSVVMFLPVRASSAAARESADSPRSARADRSPGKTKAKARAAGGAKQAAA
                     PTRAARGGKAAAAAPGKGGRKARR"
     gene            complement(40085..40855)
                     /gene="gloB"
                     /locus_tag="PKN51_01160"
     CDS             complement(40085..40855)
                     /gene="gloB"
                     /locus_tag="PKN51_01160"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNT83735.1"
                     /translation="MDLIALPAFEDNYLWLLHDGQQALVVDPGDAAPVQAALRRLGLR
                     LACILVTHHHGDHTGGVTALREASGATVYGPARERIPEPFTPLAGGDRLRVLGLDFEV
                     LDVPGHTAGHIAFFARDVDGAPLLFCGDTLFSGGCGRIFEGTPAQLLASLDALAALPP
                     TTRVCAAHEYTLGNLRFARTVEPDNTALADHERQCLQRRADGRPTLPSTIGLERAINP
                     FLRSREAAVTRAVRAHSPSANDAVAVFAALRQWKNEFR"
     gene            40943..41734
                     /locus_tag="PKN51_01165"
     CDS             40943..41734
                     /locus_tag="PKN51_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT83736.1"
                     /translation="MHHWFQTPPGQYLLAWERAQFDAALADVFGYHALQLGLADIDAL
                     AANRMPHRWLALTALPPAADEAAPSAGRWALVSDSTALPFAEASLDLVVLPHTLELSP
                     DPHATLREVQRVLVHEGKVAIAGLNPISLWGLRQWRSHLYRGLGGGQPFLPQAGEFIG
                     HWRLRDWLQLLQFELESVSFGCWRPAVRSPRRLARYAWMDRLGARWWPVFGAAYVMLA
                     VKRTHGARLIGARWKAAPVAATAPATVAHRRADAPTLTETELEPR"
     gene            41721..42158
                     /gene="rnhA"
                     /locus_tag="PKN51_01170"
     CDS             41721..42158
                     /gene="rnhA"
                     /locus_tag="PKN51_01170"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNT83737.1"
                     /translation="MNHVEIYTDGACKGNPGPGGWGVLLRAPGHEKELYGGEPQTTNN
                     RMELTAVIEGLRALKRPCEVDLYLDSQYVRQGITEWIAGWKARGWRTASKQPVKNVDL
                     WQQLDALVGGAGHRIRWHWVRGHTGHEGNERADALANRGVPRG"
     gene            42336..43601
                     /locus_tag="PKN51_01175"
     CDS             42336..43601
                     /locus_tag="PKN51_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT83738.1"
                     /translation="MVAVGALLALSAGAWWWQRAGGSAPAGGPVPAASAARGPGNGAA
                     GGAGGGAPPVVELATARAMVLSDSASAVGSLRARQSVVLRPEVAGRVTQLGFRDGERV
                     RRGQLLVQLDDRLALAQVQQARAELGIAESNHRRNADLAGQGFISARAVDESQAAVAV
                     ARAKLELAQASAARLKILAPFGALAGIGNVHVGDVLQAGADIVHLDDLDAMLLDFRLP
                     ERWQGRVRRGQISHVTLDALPGHAFEAVIQAIDPQIDAQGRSVAVRACIDNRQALLRP
                     GMFARVDTRFGERSGAVMLAEEAVLSQGGNLYVYRLAAGPAAGASAPEGTAEVERVPV
                     TLGARQAGLVEAVSGVAAGERIVVAGQQRLNAARTRVRLTRAADAAAGADGPAPAASA
                     AAAAPAPAAPLQWHRAGSGGNACAAAARG"
     gene            43720..46821
                     /locus_tag="PKN51_01180"
     CDS             43720..46821
                     /locus_tag="PKN51_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT83739.1"
                     /translation="MQLAEVSIRRPVLATVLSLLIVLIGLVSYSRLAVREYPKIDEPV
                     VTVSVRYVGASAEVIETQVTKPLEDSIAGIDAVDVLTSISRPEQAQISVRFRLEKDAD
                     TAAAEVRDRVSRVRNRLPQSIDEPVIAKVEADAFPVIWLAFSSDTRSRLEINDLVNRI
                     VKARLQTVTGVADVRIFGERKYAMRVWLDPERLAAYRLTTQDVEDAIRRNNLELPAGR
                     IESSQREFSVTSQTDLARPAQFADIVLRTVSGFPVRLRDVARVEEGAADERSAVRLNG
                     RSAISAGVIRQTTANPLLLSRGVRDMLPRLQDDLPPDVKIDIANDNSVFIDRAVSSVY
                     TTITEAIVLVALVIFVFLRTVRASIIPIVTIPVSLIGAFGLMSLAGFSINTLTLLALV
                     LAIGLVVDDAIVMLENIHRHIEEGMAPFAAAIHGAREIGFAIVAMTLTLAAVYAPLAF
                     TPGRTGRLFVEFALALAGAVLVSGFVALTLSPMMCSKLLRHNPRPGVFDRGMERALMG
                     LARAYGRLLRRVLTRARALVLLGMAGCAAAIAWLYPSMRQELSPLEDRGVILANINAP
                     DGATLDYTDRYARRLEAIGQQYPEFDRIFANIGNPSVSQGSVVYRAVDWSERRRSTLD
                     IARQMQPQFNQLAGVNAFPITPPSLGQGFRERPVNFVVQTSDSYDNLNRAMRALMDEV
                     AANPGFIAPDVDLRLSKPELRIEVDRERAADLGVSVDAVARAIETMLGGRQVTRYKRD
                     AEQYDVIVQTTASGRGTPADIDRIQLRASKGGVDTMVPLSSLVQVREGVAPRELNHFG
                     QRRSATLTANLAPDYSLGQALTFLDQAAARVLKSGYTTDLNGISREFRSSQGALVVVF
                     VLALLFIFLVLAAQFESFVDPLVIMFSVPLSMIGALLALKWSGGSLNVYSQIGLITLV
                     GLVTKHGILIVEFTNQLRQRGWDMLDALVHASSQRLRPILMTTGAMVLGAVPLALASG
                     AGSESRRQIGWVIVGGMSLGTLLTLFVVPTVYALLARRAVPGANTTPVEAPQAAQPAP
                     TGH"
     gene            46856..47359
                     /locus_tag="PKN51_01185"
     CDS             46856..47359
                     /locus_tag="PKN51_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HNT83740.1"
                     /translation="MTRPSTPRRALLAALTLCGGAAFAAGSHHGPHGEHAATDSAIGQ
                     PGVAARASRSVRIDMNDRMRFSPERLAVRQGETVRLVVRNTGQVAHELSLGTLQDLLA
                     HRDQMARQPGMTHAEPNQITLAPGQQGEIVWHFSRAGTVHFACLIPGHYEAGMRGEIQ
                     IRSSGQH"
     gene            complement(47512..48432)
                     /locus_tag="PKN51_01190"
     CDS             complement(47512..48432)
                     /locus_tag="PKN51_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNT83741.1"
                     /translation="MSVYSMTGYASGQQALAGDAEGGSGAKNARGGVGMELRSVNSRF
                     LDLSFRLADELRPHEPALRELLGQRLRRGKVELRAWLQGQADDALAEPSPRLLQRLAT
                     LEDQVCNWLPQATRLSVAEVLRLAGGTDSLSDEAAAEALRALARRVLDDFVAAREREG
                     ARLARLIGERVTQLRALARAAEPLVPQAVQAQKQRFLDRWREALASGEGQIGAEAAQE
                     RALAEATAYAIRIDVAEELGRLAAHLDEIEAVLHQGGETGKRLDFLIQELHREANTLG
                     SKAAVLELTRISVDMKVLIEQMREQVQNIE"
     gene            48525..49532
                     /locus_tag="PKN51_01195"
     CDS             48525..49532
                     /locus_tag="PKN51_01195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT83742.1"
                     /translation="MSKAKPAPLPPDTEIGGYRVLRRISAGGFGVVYLAVDREGQQVA
                     IKEYLPSALTLREPGELVPQVAPEKLSLYRLGLKSFFEEGRSLAQISHPSVVSVLNFF
                     RQNETVYMVMNYLEGATLQDFIVTARELKKQKVFRESTIRSLFDEILRGLRIVHQHKM
                     LHLDLKPANIFITDDNKAVMIDFGAAREVLNKEGNFTRPMYTPGFAAPEMYRRDSTMG
                     PWTDVYAIGACIYSCMHGYPPNDAPQRLEKDRLSLSLTRMRGVYSDNLIEVVEWCMSL
                     DPLSRPQSVFALQKELSREGERQYTRLSVGERVRLQFDNMMNDTKQSLKRVTRFGARP
                     K"
     gene            49544..50452
                     /locus_tag="PKN51_01200"
     CDS             49544..50452
                     /locus_tag="PKN51_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNT83743.1"
                     /translation="MKFSVFQLSRRGGREKNEDRMGYSYTRDAGLFALADGMGGHPEG
                     EVAAQIALQTMSALFQREARPSLAVPTSFLASSLMGAHHQIIRYASERGLLDTPRTTL
                     VAALVQEGELHWMHCGDSRLYVVRDGQLLVRTRDHSYSEAQAGAGMTSEAINRNVLFT
                     CLGSTVRPMFDIAGPLRLMQGDRVLLCSDGLWGSLDDDLIVRTLGALPVAKAVPALVD
                     VALQNAGEHSDNVTVLAFEWESPPESAFEHSGFTETQVLSDEVFASTIQAGAYDPLLD
                     DLDDEAIERSIAEINAAIQRTAMNRR"
     gene            50509..51246
                     /gene="rph"
                     /locus_tag="PKN51_01205"
     CDS             50509..51246
                     /gene="rph"
                     /locus_tag="PKN51_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNT83744.1"
                     /translation="MNASVSSPARTDRAPDQLRPVRITRHYTRYAEGSVLIEFGHTQV
                     LCTASVEEKVPPHKRGSGEGWVTAEYGMLPRATHTRGDREAARGKQSGRTQEIQRLIG
                     RSLRAVFDLTKLGERSILLDCDVLQADGGTRTAAITGAFVAAQDAVSGLLARGLIAES
                     PILHPVAAISVGIVGGVPMLDLDYAQDSSCDTDMNVVMTGAGHYVEVQGTAEGAAFSR
                     AEMDQLLRLAEKGIGELIEMQRRSLLN"
     gene            51290..51889
                     /gene="rdgB"
                     /locus_tag="PKN51_01210"
     CDS             51290..51889
                     /gene="rdgB"
                     /locus_tag="PKN51_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNT83745.1"
                     /translation="MRIVLASNNPGKLAELQAMLAPLGMDLVRQAELGIGEAAEPFRT
                     FVENALAKARHAAAASGLPALADDAGLCVDAFGGLPGVDTAYYGTQFGYAKGDAGNVC
                     ALLEQMQGIDNRRAAMVSTLVALRRADDPEPLIAVGRVVGEIARAPRGSGGFGFDPVM
                     FIPEHGQTFAEMPTDTKNALSHRGRAVAAMVALMRERWL"
     gene            51880..53088
                     /gene="hemW"
                     /locus_tag="PKN51_01215"
     CDS             51880..53088
                     /gene="hemW"
                     /locus_tag="PKN51_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNT83746.1"
                     /translation="MAVNNDVRHYLRPGTLQLPALPPLSLYLHLPWCLKKCPYCDFNS
                     HERRTGVDARAQQGRYVDALLADLEASLPLVWGRPVYSVFIGGGTPSLFEPDDIDRLI
                     AGVRARLPLEPMCEITLEANPGTFERERFAAFRAAGVTRLSVGVQSFHDRHLKALGRV
                     HDGAQARAALQEAARAFDTFNLDLMYALPGQTLAELEQDLRTALDFAPPHLSIYHLTI
                     EPNTVFAKFPPRLPDEDTAWDMLDRITALTADAGLARYEVSAYARPGHRCVHNLNYWE
                     FGDYLGLGAGAHGKLSFPHRIVRQVRHRQPERYMEQALAGDAVAQAHEVARAELPLEF
                     MLNALRLKDGFSLTDYTERTGMPPSSIASALQQAEQSGWLQREGAWIRPTARGFDFLS
                     DLQQLFLPEG"
     gene            complement(53105..53707)
                     /locus_tag="PKN51_01220"
     CDS             complement(53105..53707)
                     /locus_tag="PKN51_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNT83747.1"
                     /translation="MTAAAASEPAFSICVYCGSRPGEAQAFADVAAQVGHWIGRHGGQ
                     LVYGGGRAGLMGVVADATLAAGGRVVGIIPRVLVEREHAHRGCHELHVVETMHERKRQ
                     MAERADAFLALPGGIGTFEEFFETWTWRQLGYHDKPVGLLNQDGYYDAMLSFLQHSVA
                     SGFMGAAQMALIEVGTDWRALLGALVHATGFPAPDLLSQI"
     gene            complement(53704..54117)
                     /locus_tag="PKN51_01225"
     CDS             complement(53704..54117)
                     /locus_tag="PKN51_01225"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNT83748.1"
                     /translation="MPPTDRPREQAPAHAHQRRTGLARLWHATGHSLAGLRAGWGEPA
                     FRQEALLALVLLPAALWLGRTWVEVALLVGSLLLVLMVELLNSGIESAIDRIGPEWHA
                     LSKRAKDMGSAAVLLSLLLGAGIWLAALYTHLFSS"
     gene            complement(54144..54692)
                     /locus_tag="PKN51_01230"
     CDS             complement(54144..54692)
                     /locus_tag="PKN51_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNT83749.1"
                     /translation="MNSSAATPSTPIAVAGSPLRAPSLRRRMACWLYEGTLMFGVVVL
                     PGLVFGIATNTRHALDNRHALQALLFVVLALYFVYFWVKGQTLPMKTWHIRVVDRHGL
                     PLRWPRALLRYLLAWLWFLPPLAAMAPFRLSGSEVTVLMFGWIAVWALLSRFQREQQF
                     WHDAWAGTRLIDVQPDAARRRQ"
     gene            complement(54753..55373)
                     /locus_tag="PKN51_01235"
     CDS             complement(54753..55373)
                     /locus_tag="PKN51_01235"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNT83750.1"
                     /translation="MRHFLPVLLLALTALTLWPAHAQEVAKPGSAAAVGGQIEWRSLS
                     TAQRTALAPLERDWASLGSDQQRKWIALTQNFSRMTPQERATLQGRMTEWANLTPAQR
                     TQARLNFGEVRKVPTDERRAKWEEYQALPEEERARLARERPKPPAGAAPALRPVPAER
                     LVRPARPPVPVVLPEAPASAPVPARVNRNTLLPQRPRPAAPNTESR"
     gene            complement(55406..55873)
                     /locus_tag="PKN51_01240"
     CDS             complement(55406..55873)
                     /locus_tag="PKN51_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HNT83751.1"
                     /translation="MNTNFSQHHTESLRSRFGLRVGARLSAGTHELPHDVRERLRAAR
                     ERALAQRRQPVTQRVARPSWAAVRQGATLTLGAGDNGAGFWGRLASAALMLVLVAGLV
                     AIDVVQDNDRATEVAELDAALLTDDLPPEAYADPGFLQFIKSDLGRTSSTLSQ"
     gene            complement(55870..56436)
                     /locus_tag="PKN51_01245"
     CDS             complement(55870..56436)
                     /locus_tag="PKN51_01245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT83752.1"
                     /translation="MATDQELSDFLRNVEKRAFKRTVYHVRDDDAALDIVQDSMMKLA
                     EHYGDKPAAELPLLFQRILSNSTLDWFRRQKTRNALFSNMSDFDAEDGDGEFDLLEIF
                     AGASDSTQIESAEDATRRAQVLHEIELQIQALPNRQREAFLLRYWEEMDVAETAAVMG
                     CSEGSVKTHCSRAVQALAKALRAKGIQP"
     gene            56738..58525
                     /locus_tag="PKN51_01250"
     CDS             56738..58525
                     /locus_tag="PKN51_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HNT83753.1"
                     /translation="MEISKAELKSAAVAASADTSQAPQELRGAEILIKALQAEGVKYV
                     WGYPGGAVLHIYDAFYKQDTIQHVLVRHEQAAVHAADGYARATGDVGVALVTSGPGVT
                     NAVTGIATAYMDSIPMVIITGQVPTAAIGQDAFQECDTVGITRPIVKHNFLVKDVRDM
                     AEVMKKAFHIARSGRPGPVVVDIPKDVSFEKTAYKGYPQSVEMRSYNPVRKGHGGQIR
                     KALQLLLAARRPYIYTGGGVLLADACAELRTLVDLLNCPVTHTLMGLGAFPASDRRFL
                     GMLGMHGTWEANNAMQHCDVLLAVGARFDDRVIGNPKHFAQNERKIIHIDIDPSSISK
                     RVKVDVPIVGDVKDVLTEMIGMIREAGARPDEAALADWWKTVEGWRARDCMKYDRANT
                     DVIKPQKVIETLWEMTRGADAYITSDVGQHQMWAAQYYRFDEPRRWINSGGLGTMGVG
                     IPYAMGIKLARPDSEVYCVTGEGSVQMCIQELSTCLQYNTPIKIISLNNRYLGMVRQW
                     QELQYAGRYSHSYMDALPNFVKLAEAYGHVGMLIERPQDVEPALREARKLKDRTVFMD
                     FRTDPTENVFPMVEPGKGITEMLLGSEEL"
     gene            58655..59146
                     /gene="ilvN"
                     /locus_tag="PKN51_01255"
     CDS             58655..59146
                     /gene="ilvN"
                     /locus_tag="PKN51_01255"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNT83754.1"
                     /translation="MKHIIAVLIENEAGALSRVVGLFSARGYNIESLIVAPTEDPSLS
                     RMTIQTRGSDEVIEQITKHLNRLIEVVKVVDLTEGSYIEREHMMVKVRAVGKEREEIK
                     RMADIFRGRIIDVTDKSYTIELTGDQGKHDAFLNAIDKSAILETVRTGASGIGRGERL
                     LKV"
BASE COUNT         9063 a        21027 c        20600 g         8486 t
ORIGIN      
        1 ccgcagaacc agggcgcctg gttcttcatc cagcacaaca tccacgagaa catgcacgaa
       61 ggtcagaagc tgggctacgc cggccgggcc gcctcggcat cacccgccgt gggttacgcg
      121 cacctgcacc aggaccagca gaaggcgctg atcgatgccg ccttcggccg tctgaaaggc
      181 ttcgtgctgc agaagtaagg atggaggttg ccgggcccgg gctgtagcgc ttccgccagc
      241 ttccgccctc gcgccggcgc cagtcgcaac cgcattcaag aacaccggaa gaatcccaaa
      301 tggctatcgt cgaagtcaaa gttccccaac tgtccgaatc cgtggccgag gccacgctgc
      361 tgcaatggaa gaagaaggcc ggtgaggccg tccaggtcga tgaaattctg atcgaggtcg
      421 aaaccgacaa ggtggtgctg gaagtgcccg cgcccgccag cggcgtgctg accgagatcg
      481 ttcaggccga cggcgccacc gtggtggccg accagctgct ggcgcgcatc gacaccgccg
      541 ccgtggccgg ggccgccgcg cccgccccgg ccgccgcgcc gagcgccgct cctgcgcccg
      601 ccgccgcccc cgcggccgac aagtccggcg tggccatgcc cgccgccgcc aagctgatgg
      661 ccgaccacca gctgcccgcc ggcagcgtgg ccggcaccgg caaggacggt cgggtgacca
      721 agggcgatgt cctgggcgct attcaatcag gagctgctgg cgcaggcggg gcaagctctg
      781 gagcccaaaa agctgctcag atcccgaccg gtgtgccaac caccgcgctg ccccaggtgg
      841 cggccccggc ggcgccccag ttgggcgacc gccccgagca gcgcgtgccc atgacgcgcc
      901 tgcgcgcgcg cgtggccgag cgcctgctgc agtcgcaggc cagcaacgcc atcctgacca
      961 cgttcaatga agtgaacatg cagccggtga tggacatgcg caagcgcttc caggacaagt
     1021 tcgagaagga gcacggcgtc aagatcggct tcatgagctt cttcgtcaag gcggcggtgc
     1081 atgcgctcaa gaagtacccg gtgctcaacg ccagcgtcga cggcaacgac atcgtctatc
     1141 acggctactt cgacatcggc attgccgtcg gctcgccgcg tggcctggtg gtgccgatcc
     1201 tgcgcaacgc cgaccagatg agctttgccg acatcgagaa gaagatcgcc gagttcggtg
     1261 ccaaggcgcg cgatggcaag ctgggcatcg aggaaatgac cggcggcacc ttctcgatca
     1321 gcaacggcgg cgtgttcggc tccatgatgt ccacccccat catcaacccg ccgcagtcgg
     1381 ccatcctggg cgtgcacgcc accaaggacc gcgccgtggt cgagaacggg caggtcgtgg
     1441 tgcgccccat gaactacctg gcgatgagct acgaccaccg catcatcgac ggccgcgaag
     1501 ccgtgctggg cctggtggcg atgaaggaag cactggaaga cccggcgcgc ctgctgttcg
     1561 acatctgatc ggatcacccc acggcccttg gcgggccgtg cccctgtggc gggctgggcc
     1621 cggaacggaa caacgcacat gagcaaccaa caattcgacg tcatcgtcat cggcgccggt
     1681 cccggcggct acatcgccgc catccgcgcg gcacagctgg gccagaaggt ggcctgcatc
     1741 gacgagtgga agaacgacca gggcggtccg gccccgggcg gcacctgcac caacgtgggc
     1801 tgcatcccgt ccaaggcgct gctgcagtcg tccgagcatt tcgagcacgc caacaagcac
     1861 ttcgccgagc acggcatcag caccggcaag gtgacgatgg acgtggccaa gatggtgggc
     1921 cgcaaggacg ccgtggtgaa gcagaacaac gacggcatcc tgtacctgtt caagaagaac
     1981 aaggtcagct tcttccacgg ccgcggatcg ttcgtgcgcg ccgtggatgg cggctacgag
     2041 atcgccgtca agggcgccaa ggatgagacc ctggtcggca ggcagatcat tgtcgccacc
     2101 ggctccagcg cgcgtgcgct gcccggcgtg ccgttcgacg aggagcaggt gctcagcaac
     2161 gacggcgcgc tgcgcatcgc tgccgtgccc aagcgcctgg gtctgatcgg ctcgggcgtg
     2221 atcggtctcg aaatgggcag tgtgtggcgc cgcgtgggcg ccgaagtcac ggtgctggaa
     2281 gccttgccga ccttcctggg tgcggtggac gagcagatcg ccaaggaagc gcgcaaggcc
     2341 ttcgacaagc agggcctgaa gatcgagctg ggcgtcaagg tgggcgaggt caaggtcggc
     2401 aagaagggtg tcagcgtcgc ctggaccgac gccaagggcg cagcgcagga gctggtggtc
     2461 gacaagttga tcgtctccat cggccgcgtg cccaacacca ccggcttgaa cgccgaggcc
     2521 gtgggcctca ggctcgatga gcgcggtgcg gtggtggtgg acgacgactg ccgcaccaac
     2581 ctgcccggcg tctgggcggt gggcgacgtg gtgcgcggcc ccatgctggc gcacaaggcc
     2641 gaggaagagg gcgtggcggt ggccgaacgc atcgccggcc agcacggcca cgtggacttc
     2701 aacctgattc cctgggtcat ctacaccagc cccgagatcg cctgggtcgg caagaccgag
     2761 cagcagctca aggccgaggg cgcgaagtac aaggccggca gcttcccctt catggccaac
     2821 ggccgggccc gcgcgctggg cgacaccacc ggcctggtca agttcctggc cgacgccgcc
     2881 accgacgaaa tcctgggcgt gcacatcgtc ggccccatgg ccagcgaact gatcgccgag
     2941 gcctgcgtgg cgatggcgtt ccgcgccagc agtgaagaca tcgctcgcat ctgccatgcc
     3001 cacccctcgc tgggcgagag catgaaggaa gctgccctgg cggtcgacaa gcgcaccctc
     3061 aatttctgat gaaatcggcc attggcgctt gtgcagaaag cgcgagcagc tatttttttc
     3121 atagcatcgg tggtctgatc gcatgagcga tgtgcgccag gcctacgagc tcgagctgaa
     3181 ggcgcgtggc ttccagagcg acccggcgca gctgcgcgcc gtgcaggcgc tgcagcgctg
     3241 cgccgacgac tgggcggcct acaagtccag ccgttccaac cggctcaaga agctcttcaa
     3301 ccgccccgag atcccgcgcg gcgtctacat gtacggcggg gtggggcgcg gcaagagctt
     3361 cctgatggac tgcttcttca acgccgtgcc gatccggcga aagacgcgcc tgcactttca
     3421 cgaattcatg cgtgaggtgc accgcgagct ggcggcgctg cagggcacgg tcaacccgct
     3481 cgacgagctg ggccggcgca tggccaagcg cttcaagctg atctgcttcg acgagtttca
     3541 cgtcgccgac atcaccgacg ccatgatcct gcaccgcctg ctggtggcgc tgttcgacaa
     3601 cggcgtaggc ttcgtcacca ccagcaactt caagcccgac gacctgtacc cgggcggcct
     3661 gcaccgcgac cgcatcctgc cggccatcga gctgctgaac cagcgcatgg aggtggtcaa
     3721 cgtcgacaac ggcaccgact accgccaccg cacgctgcag caggtgcagc tctaccacac
     3781 gccgctgggc gcggcggccg acgccgccat gcaggcggcc ttcgaccagc tggccagccg
     3841 cgccgacgaa gacccggtgc tgcacatcga ggcgcgcgag atcctggcgc gccggcgcgc
     3901 cggtggtgtg gtgtggttcg actttcgagc gctgtgcggc ggcccgcgct cgcagaacga
     3961 ctacctggaa atcgccagcc agttccacac cgtgctgctg tccggcgtgc cgcacatgcc
     4021 ggtgcgcatg gcgtcggagg cgcggcgctt cacctggctg gtggacgtgc tgtacgaccg
     4081 ccgggtcaag ttcatcctgt cggccgaggt gccgcccgaa cagctctaca ccgaaggtcc
     4141 gctggcgcac gagtttccgc gcaccgtcag ccgcctcaac gagatgcagt cggccgagta
     4201 cctggcgctg gagcatcgca ccgtggacac acaactgaca tgagccgcct gcccatcagc
     4261 ctgctgctgt gcaccctgct ggccgcctgc gcgtccggct cgctgcacga tcagcacctg
     4321 gcccggcgcg ccgccgacgc cgccgacgcg caggccctgg ccgccacggc gccgccggcg
     4381 ggccagccgc tggcgccctg gctgcaggcg cagcgtgccc gcatcgtcag cgagcgcggc
     4441 gcagccacgc gccacttcga ggaggccgag aaagcctgct ggcagcgctt tgccgtcaac
     4501 gactgcctgc agcaggcgcg cgtgcagcgg cgcacggcgc tggaccggct gcgccagcag
     4561 gaactcgcca tcaacgatct ggagcgccag cgtcaggccg atgcgcgccg gcttgaacct
     4621 agaaacggca gttgaccgct tgtcatcttc gggcaggccg catccctgca gggtttggcg
     4681 gctccgatgg ccttcaccgc ctgggtgagc acgctgcgca cccacccgca ccgcgctcgg
     4741 cgcgccatgc ggcgttgctc cttcttgcgg gcacgagccc gcggcggcgt tgcgtcttgc
     4801 ctggcgcgcc gatcacggca cgggcgggcg cgtccaactg ccgtttctag gaggctgtcg
     4861 ggcttggagc gccgaaagcg ttctcgaaga gcggcgtagc caaaatcggc cccagcgaga
     4921 ccagtttttg ccggatttgc agcccaatag ccgagctatt gggcaaaaag ccggtgaaaa
     4981 atgggccgct gcggtcgatt tgcagccggc gatctccaag tccgacagcc tcctaggttg
     5041 aactggagcg gcaggagtcc ggcgcgacgc gccgatgagt gcatggcgct caagccgcga
     5101 agcacggtga gcacatccgc ggcgcaccgc tgaccgaggg ggtcaacgcg cccatgccgg
     5161 gcgcgggcgc ggtcagcgca gtggcgagaa ggccgagccc accaccggct ggttggtctg
     5221 aagcggctcg accttgacga tggccagcgc caggttgtcg ccgccgccca tggcgcgcgt
     5281 gcgtgccttg tcgatcagga attcgctggc ctcgcgcggt ggcagcgaat gcagcaccga
     5341 acccagctct tccggcgtga agtagtgcca cagcccgtcg gtgcaggcca gcagggcgtc
     5401 gccgatctgc agctggggga tgtagtgcac gtcgaacttg ggcgcttctt cggtgcccag
     5461 gcagcccagc agcacgttgg acttggggtg cacggcggcc tcgtcttcgg tcagctcgcc
     5521 ctggtcgacc agcgcctgca cgtaggactg atccttggtg cggcgcacca ggcgggcgcc
     5581 acgaaaatgg tagagccgcg agtcgccggt gtgcgcccaa tggcattcgc cgcccacgtt
     5641 gatgatgaag gccgccatgg tgctgtgcgg ctcctgctcg gcggcgatcg ccgtcagctt
     5701 gatgatcagg tgcgcttcct cgccgatctg gcgcagcacc gcggtggcgt cttcgctgtc
     5761 gggggcgtag cgctcgaaca gctggcgcgc cgtcagcatc acctggtcgg cggccttgcg
     5821 cccgccggtg cgcccgccca tgccgtcggc caccacgccc atcacgcagc cgttggcgcg
     5881 cgcgtgggcg aacagggcca cctggtcctg ctggtagtcg cggtcgccct tgtgcagacc
     5941 ggtggcggcg ctgagtcgat agcctttttc catggtgccc cttgcagagg cggcggggta
     6001 cgccgcaagt cgtattattg aatgcggtgg cacggaaggc ccggcgccgt gtcggcggca
     6061 agtgcaatcc ttgtgcttta gtacgcaatc ttacacatga cggccaacct gcattccccc
     6121 gagcgccggc tcatcgagct gcgcatggag catgccgatc tcgacgcctc gatcgaccgg
     6181 ctggcgggtc aggcgccgct ggacgagctg atgctgcaac ggctcaagaa gcgccgcctg
     6241 gcgctgcgcg acgccatcgc gcggctgcag gcgcagctgg agcccgatga accggcctga
     6301 acgcgtgtcg ccagtggcca cccccggcgc gccgcacggg cccgagcccg gggcgctggc
     6361 gcaggcggtc ggggccgcct tcgacgatca cgggccgctg tcgcaggcgg ccgagggctt
     6421 tcgcctgcgc gatggccaga cccggctggc gctggcgctg gcgcgggtga tcgagcaggg
     6481 cggggtgctg gtggccgagg ccgccaccgg cgtcggcaag acctttgcct acctggtgcc
     6541 ggccctgctg tcgggcgagc gggtgctgct gtcgaccgcc accaagacgc tgcaggacca
     6601 gctgttcggg cgcgatctgc cgcggctggc gcgcgcgctg gggctgccgg tgagcatggc
     6661 gctgctcaag ggccgcgcca gctacctgtg cctgcaccgc ctcgaaatgg cgcatcagac
     6721 aggggtttca tcggcgcctg gtgtgcgcca gcagctatca aaaatagagc gttggtcgaa
     6781 gatcacgcgc agtggcgacc tggccgagct gcctgatctg gacgagcgct cgccggtcat
     6841 cccgctggtc accagcacgc gcgagaactg tctgggcgcc gactgcccgc agtttcgtgc
     6901 ctgtcacgtg cagctggcgc ggcgccaggc catggcggcc gacgtggtgg tgatcaacca
     6961 ccacctgttc tttgccgatc tggcgatccg cgaatcgggc atggccgagc tgctgcccag
     7021 cgtgcgcgtg gcggtgttcg acgaagcgca ccagctcaac gaaaccggca tccagtttct
     7081 cggccagcag ctggccagcg gtcagttgct ggactttgcg cgcgatctgc tggccgccgg
     7141 cctgcggcag gcgcgcggcc tgtgcgactg gcaggcgctg gccagcgcgg tcgaggtggc
     7201 cacgcgtgag ctgcgcctgg cggccggtgc ggggcgcgcc gccggccggc tgcgctggct
     7261 ggaagcggtg cccgaggggg tcgatgccgc cgcctggccg ccggcgctgc ggcagctgca
     7321 ccaggcgctg gccgacgccg ccgccgcgct ggccacggtc agcgagctgg cccccgactt
     7381 cgtgcgcctg caccagcgcg cgctgctgct ggcgcagcgc gccgaacgct tcggtcagcc
     7441 gtgcgagccg gcgctggtgc gctgggtcga cgttggtgcc tcactgcggc tggtggagtc
     7501 gccgctggac atcgccgaca ccgtgcgcac ccgcctgctg gccgggccgc aggacgacgg
     7561 ccgcgcgcgc gcctgggcgt tcgtgtcggc cacgctgggc gacgacgagc gcctgtcctg
     7621 gttcaccgag ccctgcggcc tgcaggacgc cgaggtgctg cgcatcggca gcccgttcga
     7681 ctacgcgcgc caggccgcgc tgtacgtgcc gacccacatc gcccgtccgg ccgacccggc
     7741 gcacagcgcc gacgtggcgc gcctggcggg cgacgcggcg gcccgcctgg gcgggcgcac
     7801 catggtgctg accaccacgc tgcgcgcgct gcgccgcatc ggcgagctgc tgggcgagcg
     7861 cttcggcgcc ggcgcggaca tcgaggtgct gatgcagggc agcctgccca agcgcgtgct
     7921 ggtggagcgc tttcgtgccg gtggcagcgc cggtcgtccg ggctgcgtgc tggtggcctc
     7981 ggcctcgttc tgggaaggtg tcgacctgcc gggccaggcg ctgcaattgg tggtgatcga
     8041 caagctgccg tttccgcccc cgggtgaccc gctggtggaa gcgcgctgcc gtcgcctgca
     8101 agcgcaggga cgcagcgcct ttgccgacca ttcggtgccc gaggcggcgg tggcgctcaa
     8161 gcagggcgcg ggccggctga tccggcgcga aagcgaccgc ggcgtgctgg tggtctgcga
     8221 cacgcgcctg gtgcagatgg gctacggtcg ccgcctgctg gcggcactgc cgccgatgcg
     8281 gcggctgccg tcgccgcagg ccttcgaggc cgcgctggac gagttgctcg ccgaagatgc
     8341 ttccaaaaca atagctgctg gcgcttgata cgcaagcgct ggagccagat ttgacagcta
     8401 ttccgcgaga tccgggctag gccgcccgct caccacagct gccaccaggg cttgtcggcg
     8461 ccgcgcaggc cgtgcgtcag gtaggtgctg ttggggtagt tcctgtcgag cacgcggcgc
     8521 gcgtcgtcgc gcaactcggt catgcccaga cggtcgtacg actgggtgag gatgtacagc
     8581 gcctcttcca gcgacggcgc atcgcggtag tcggccagcg cctgctgggc gcggttgatc
     8641 gccgccacgt aggcgccgcg cgtgaagtag tagcgcgcca catgtacttc gtattgcgcc
     8701 agcgcgttga tcgtgtagcg catgcgcgcg cgcgcgtcgg gggcgtagcg cgaatcggga
     8761 aagcgcgtca ccagttcgtt gaacgattcg aaggcttcct tggccgcctt ctggtcgcgc
     8821 tcggacagat cctggcgtgc caggaaagag agcatgccca gctcgtcgtt gaaattgatc
     8881 acgcccttga gaaacagcgc gtagtcgatc gccgggctgg ccgggtgcag gcgcatgaag
     8941 cgatccagcg tggctatcgc ctcggccttc tggccgtcct tgaacagtgc gtaggccttg
     9001 tccagctgcg cctgctgagc cagcgtggtg ccggcggcac ggccttccag ggtttcgtag
     9061 agcgggatcg ccttgtcgta ggcaccggcg ttgagctcgt ccttggcctc ggcgtggatg
     9121 cggttggggc tccagccggc ggtccggtcg atcggcttgg acgagcaggc cgcaagcagc
     9181 accgccagcg ccgccgcgac ggcgcctgcg accgataatc gagcaccttg catgagcggc
     9241 taccttcttg aatcgagcaa cggcaattat atcgggcacc cccccgtcga ccgcacccgc
     9301 tgacgccgac cgggacgccg acggcgaacc gctggaagac gccggcgccg ccgccgagct
     9361 gcgccagctg gcgctgccgg ccgagctgca cggccagcgc ctggatcgcg cgctggccgc
     9421 gctggtgccg gagttctcgc gcaactacct gcgccagctg atcgaagccg gcgtggtgca
     9481 catgcagggg cagccggtgg tcaagccggc gcaaaaggtg cgcgccggcg accatctggc
     9541 catcgagctg cgccccacgc ccatgagcca ggccttcgtg ccgcaggcca tggcgctgga
     9601 cgtggtgtac gaagaccagc atctggcggt gatcgacaaa ccggccggcc tggtggtgca
     9661 cccggcgccc ggccactgga gcggcaccct gctcaacggc ctgctggcgc gcgacgccgc
     9721 cgccgcccac ctgccgcgcg ccggcatcgt gcaccggctc gacaaggaca ccagcggcct
     9781 gctggtggtg gcgcgcagcc gggcggccat ggacgcgctg gtggcgctga tcgccgcgcg
     9841 ccaggtgcgg cgcgaatacc tggcgctggc gcaccggccc tggtccggcg cgccggcgcg
     9901 cgagatcgac gccgccatcg gccgcgaccc gcgccagcgg ctgcgcatgg cggtgctgga
     9961 ccccgcgcgc catccggcca ggccggccca gacccgcttc gagctgctgc agaacgcgcc
    10021 ccagggctgc ctgctgcgct gcctgctgcg caccggtcgc acgcaccaga tccgcgtgca
    10081 tctggcacac ctgggccatc cgctggtggc cgatgcgctg tatggcggcg ccccggccgc
    10141 cgggctgacg cgccaggcgc tgcatgcgcg ccggctggcc tttcggcacc cgctgaccgg
    10201 ccagacgctg gatttcgtgg cgccgctgcc gaccgatctg acgcacgcac tgagcgcctg
    10261 gggccttaca atgcccgctg acgacatgga cccggcggcc agccccgcgc gttgagtcca
    10321 tcacggcccg cagcccgcgg cccctggccg gtgtggccgg ctcgcttgcc gagcccgcgc
    10381 gcaccggtct ccagacagac acggaacacc ctcgctgtga attccacgga cgccaagcgc
    10441 atcctcgaaa ccgccctgat ctgcgccccg cagcccatgc cgctgcgcga actgcgcgtg
    10501 ctgttcgacg acgaactggg cgccgacacg ctgaagaacc tgctgctgga gctgcaggac
    10561 gactgggccc agcgcggcgt cgagctggtt caggtggcca gcggctggcg cttccagagc
    10621 cggcccgaga tgcgcgagta cctcgaccgc ctgcaccccg aaaagccgcc gcgctacacc
    10681 cgcgccacgc tggagacgct ggccatcatc gcctaccgcc agccggtcac gcgcggcgac
    10741 atcgaggaca tccgcggcgt caccgtcaac acccagctca tcaagcagct ggaagaccgc
    10801 ggctgggtcg aagtcatcgg ccaccgcgaa accgtcggcc gtcccggcct gtacgccacc
    10861 acgcgccagt tcctcgacga cctggggctg gagtcgctgg atcagctgcc gatgctggac
    10921 accccctccg agcaggccgc cagcatgttc gacgccctcg gtcaggccgc cgaggacgcg
    10981 ccggccgctg ccgccaccga cgccgagccg gccgccacgc cggacgtcga ggcgggtgag
    11041 cccgccgccg cgcccgccgc caaggccgaa cccgaatccc catccgcacc cgacgccgac
    11101 gacagcgccg cgcccgccgc caccccggcg tcggcgcccg agcccgagcc cgtggacgac
    11161 agcccgccgt cccgccccga caccgccgaa cacgaggccc cccatgtgcc ctgacgagcc
    11221 gaccgcgcct gccgccgacg accaggcttc ggccaccccc ggacccgccc ggcgatccag
    11281 aacaaaaccg acttcggagc ccgcccagac tgcgccgaat gctactgata tggtagcaat
    11341 cgaggccgac gccgaaccca ccggggcgcc gacggccgcg gaccaggcgc cggtcgtgat
    11401 cgacgacgtg ctgtccggcc gcttcgacgc cgaagccgac ggcggtgcgt ccgacgtccc
    11461 gcccaagcgc gtgctgctgc cgcagcccga gcagcccaag ctgcacaagg tgctggccca
    11521 ggccgggctg ggctcgcgtc tggagatgga gcagctcatc ctgcaggggc gcatctcggt
    11581 caacaaccag ccggcccaca tcggccagcg catccagttc ggcgatgcgg tcaagatcaa
    11641 cggcaagccg atcaagctgc gcatcgcgcc gccgccggcg cgcgtgctgg cctaccacaa
    11701 gccggtgggc gaggtggtga cgcacgacga cccgcagaac cgccccacgg tgtttcgcaa
    11761 gctgccgcgg ctgccgcagg gcaagtggca gtcggtcggt cggctggacc tgaacaccga
    11821 aggcctgctg ctgctgacca attccggcga gctggccaac cgcctgatgc acccgcgctt
    11881 cggcctggag cgcgaatacg ccgtgcgggt gctgggcgcg ctgtccgacg aggaaaagca
    11941 gcagttgctc gatggcgtgc cgctggacga cggcatggcg cagttcagct cgatccagga
    12001 agaagggcag ggcgaaggcg ccaaccagtg gtaccgcgtc accatctccg aaggccgcaa
    12061 ccgcgaggtg cggcgcatga tcgagtccgt cggtcacgcc gtcagccggc tgatccgcat
    12121 ccgctacggc gccatgatgc tgccgcgcgg cctgcggcgc gggcagtgga tggaactggg
    12181 cgagcgcgac atccgcgccc tgggtgcggc ctcgggcacg cccgagcatg tgctgcgcgg
    12241 cggccgtgag cccgggccca ggccgtccga gccgccgcgc cgtggtcgcg gcgaccgcgg
    12301 ccgcggcagt gggccacgcc ccaacatggc cggtggcggc cggccaccgg cgaacaccgg
    12361 cagcggcaag ccgcgccggc gcgacggcgg gccggccggc ggcgccggcg cgccgcaacc
    12421 cgatcccatg aagacctcgt tcggctacat cggcgccgac agcctgtcgc gccagcgcca
    12481 gagcgccggc cggggcgcac cgggcgcgcg cggcggtggc ggccgggcgc gccgcggccc
    12541 agctcccggc ggccggcgtg gctgagcggc cggcgccatc ctgcgaccgt cccgcccgcc
    12601 gccgcgaccg cgggtgggcc ggttaaaatc aatggctttg cccagccggg cagcagatcc
    12661 ttaccgagaa ccttagaaag cacctccatg gctatcgaac gcaccctgtc catcatcaag
    12721 cccgacgccg tcgccaagaa cgtcatcggc cagatctacg cccgcttcga ggccgccggc
    12781 ctgaagatcg tcgccgcccg catgcagcac ctgtcgcgcc gcgaggccga gcagttctat
    12841 gccgtgcaca aggagcgccc cttcttcaag gacctggtgg agttcatgat ctccggcccg
    12901 gtgatgatcc aggtgctgga aggcgagggt gccatcgcca agaaccgcga cctgatgggc
    12961 gccaccgatc ccaagaaggc cgacaagggc accatccgtg ccgacttcgc cgattccatc
    13021 gacgccaacg ccgtgcatgg ctccgacgcg cccgagacgg ccgccgtcga ggtggcgttc
    13081 ttcttctccg gcatgggtgt ctacagccgc tgagccggcg caaggcggcc cgcgcagcgc
    13141 atgaccacgc ccgtcaacct gctcgacttc gacctcgaag gcctggccgc ctggtgtgac
    13201 acgctgggcg aaaagcgtta tcgcgccacc cagctgtttc gctggatcca tcagcgcggc
    13261 gtcgccgagt tcggccagat gagcgatctg gccaggtcgc tgcagggcaa gctgcacggc
    13321 cgcgccctgg tggcggcacc ggccgtcatc cgccagcacg cctcgaagga cggcaccatc
    13381 aagtggctgt tcgacgtcgg tggcggcaac gcggtcgagg cggtctacat cccggaagac
    13441 gaccgtggca cgctgtgcgt gtcgtcgcag gccggctgcg ccgtggcctg tcgtttctgc
    13501 tccaccggtc accagggctt ttcgcgcaac ctgagcacgg ccgagatcgt cggccagctg
    13561 tggtttgccg agcattttct gcgccgccac ctgaagaccg acgagcgcgt catctccaac
    13621 gtggtgatga tgggcatggg cgagcccctg cagaactacg accgtctgct gccggcgctg
    13681 cgcaccatgc tgcacgacca tggctatggc ctgtcgcgcc ggcgcgtgac ggtgtccacc
    13741 tcgggcgtgg tgcccatgat cgagcgcctg gcgcgcgact gcccggtggc gctggcggtg
    13801 tcgctgcacg cgcccaccga cgcgttgcgc gaccatctgg tgccgctcaa ccgcaaatac
    13861 ccgatcgatg agctgctgcg cacctgcacc gcctacctgg cgcacgcgcc gcgcgacttc
    13921 atcaccttcg aatactgcat gctcgacggc gtcaacgact ccgccgagca cgcccaggcg
    13981 ctggtcgagc tgatgcggcg gcacgccgcc agcggcctgc gcggcaagtt caacctgatt
    14041 cccttcaacc cgttcccgca gtcgggactg acgcgctcgc cgatggcgcg cgtgcaggcc
    14101 ttcggcaagc tgctgaacga cgccggcatc gtcaccacca tccgcaagac gcgcggcgac
    14161 gacatcgacg ccgcctgtgg ccagctggcc ggggacgtgc aggatcgcac gcgcgtgggc
    14221 gtgcgcatcg cgcgccagcg cacggtggcg ctgcaccagc tggcgcccgc aggcgccgcc
    14281 ccggcgccga gaaaggtgag ttcatgagcc ttcatgcttg cgccccgagc gcggcttcac
    14341 gccggcggct gctggccctc gccagcttcg gtctggcggc ctggctgctg gccgcttgcg
    14401 ccgctccgcc cggtctgggc gacaacccgc aggagctgcc caccgcatcc gacgagaccg
    14461 acgcccgtcg gcgtgcccgc acccggctgg cgctggccac cggttactac gagaacggcc
    14521 agcacaccgt ggcgctggac gagcagaaaa aggcgctgca ggccgacccg aacttcggcg
    14581 atgcctacaa cctgggcggt ctgatctaca tggcgctggg tgaccaggcg ctggcgcaat
    14641 cgcatttcca gcgcgccatc gcgctcaacc cgcgcgacgg cgacgccctg cacaacctgg
    14701 gctggctgca atgccagcag gggcagctgg cggcggccaa ccagtcgttc gagcgcgcac
    14761 tggcggtgcc cacctacccg ggccgcgcgc gcacgctgat ggcgcagggc gtgtgcgagg
    14821 cgcgcgccgg caacctggcg cgcgccgagg cggcgctgat gcgctcctac gagctggacg
    14881 ccggcaaccc ggtgactgcc ttcaacctgt cgcagctgct gtaccagcgc ggcgactaca
    14941 cgcgggcgca gttctacgtg cggcgcctga acaacagcga gctggccaat gccgaatcgc
    15001 tctggctggg catccgcgtc gagcggcgtc tgaacaaccg ccaggccatg gagcagctgg
    15061 cgtcccagct gcgccggcgc ttcccgcagt cgcgcgaact gctggcctac gagcgcggag
    15121 ccttcgatga ctgaagccca caccgggcca gccagcctgc acgacacccc ggcccacggc
    15181 gccggcccgc gttcggccgg gcagatgctg cgcgcgctgc gcgaatcggc gggcgtcgat
    15241 ccggcgctgc tggccagcgc catgaaggtg tcgccgcaga agctcgacgc gctcgaaaac
    15301 gaccgcctgg acgaactgcc cgacgtcact ttcgcgcgcg cgctggcctc ggccatctgc
    15361 cgtgccttcg gcaccgatcc ggcgccggtg ctggagcgca tgccggtgtc ggcacccggt
    15421 ctgcgcccag tggcgcaggc gccgatcaac gagccgttcc gtcgcagcag cgacgggccg
    15481 gcgacggtgc tggcgggggg gctgtccaag ccgctggcga tcggtgtcat cgccttgctg
    15541 ctgggggccg cggcgctgtg gttgctgccc acgctgccga tccagctgaa tgctcccgcg
    15601 ccggccagtg agccggccgc gaccgacggc gtggtgcgcg aaagcgtcat gccgccggcc
    15661 gccaccagtg ccgagccacc cgctccgccc gccccggccg aactgtcggc cagcgcggcg
    15721 gcggctgaac aggcgccgcc gccggccgct gccgccccgg cggcggccag caccgatctg
    15781 ctgtatttca gcgctcaggc cgaaacctgg gtcaccgtgc gcgacgccag cggcaagaac
    15841 ctgatcaacc gggcgctgaa cgccggtgac agcgtgggcg tgagcggtga gccgccgctg
    15901 gcggtcacca tcggtcgcaa ggatgcggtc gaggtgcgcg tgcgcggcga gccgttcgat
    15961 caccgctcga tcagcaagac gccggtgtcg cgcttcgagg tccgatgagc atgccgtcac
    16021 ccaactgcac accgatcgag gtggcccggc cggcgccgcg ccgcacccgc caggcgcgcg
    16081 tggtctgggg ccggcgtgtg gtgacggtgg gcggcgacgc gccggtgcgc gtgcagtcga
    16141 tgaccaacac cgacaccgaa gatgccatcg ccaccgccat ccaggtcaag gaactcgcgc
    16201 tggccggcag cgagctggtg cgcatcaccg tcaacacgcc cgaggcggcg gcgcaggtgc
    16261 cctacatccg cgagcagctc gaccgcatgg gcatcgacgt gccgctgatc ggcgacttcc
    16321 actacaacgg ccaccgcctg ctgaccgagc acccggattg cgcgcaggcg ctgtccaagt
    16381 accgcatcaa cccgggcaac gtgggcaagg gcgccaagcg cgacacccag ttcggccaga
    16441 tgatcgaggc cgcgctcaag tgggacaagc cggtgcgcat cggcgtcaac tggggcagcc
    16501 tggatcagga cctgctggcc ggcctgatgg acgaaaacag ccgccgtgcc cagccctggg
    16561 acgcgcgcca ggtgatgtac gaggcgctga tcagctcggc cctgggatcg gccagccagg
    16621 cggtggagct cggcatggat ccggcgcagg tcatcctgtc gtgcaaggtc agcggggtgc
    16681 aggacctgat cgcggtctac cgcgagctgg ccagccgcgg cgaccatccg ctgcacctgg
    16741 gcctgaccga ggccggcatg gcgacgcgcg gcacggtggc ctccagcgtg gccctggcga
    16801 tcctgctgca ggaaggcatc ggcgacacca tccgcgtcag cctcacgccg cagccgggcg
    16861 aggcgcgcac gcaggaagtg gtggtggcca gcgaaatcct gcaggcgctg gggctgcgcg
    16921 ccttcgtgcc cagcgtcacc gcctgcccgg gctgcgggcg caccaccagc accgtgttcc
    16981 aggagctggc caagcagatc gacgaccatc tgcgcgcgca gatgccgctg tggcgcagcc
    17041 agtatccggg cgtggaaaag ctcaaggtgg cggtgatggg ctgcatcgtc aacggcccgg
    17101 gcgagagcaa gcatgccgac atcggcatca gcctgccggg caccggcgag gcgccggcgg
    17161 cgccggtcta catcgacggt gagaaagcca tgacgctgcg cggcgaacac atcgcggccg
    17221 agttccagca gatcgtggcg cgctacatcg agcagcgctt cgggcgccgg caaccctgag
    17281 cggttttgag tcaaattcgg ctcaaaccag cacccatcgt gcgcaagcag ctatcaaaac
    17341 catagttatt caagtatgtc tggcagcaag cagatcaccg ccatcaaggg catgaacgac
    17401 atcctgccgc cggcgtcggc gcgctgggac tggctggagg acgtggtgcg ccgcgtgatg
    17461 gcgcagcacg cctaccgcaa cgtgcgcacg cccatcatgg agctgacgca gctgtacacg
    17521 cgcggcctgg gcgaagtgac cgacatcgtc gagaaggaga tgtacgcctt cgaggaccgc
    17581 gccgaccagc acggccgcgc cgaccacctg gcgctgcgcc ccgaaggcac ggccggcctg
    17641 gtgcgcgccg tggccgagca caacctgctg tacgacggcg gcaagcggct gttctacatg
    17701 ggccccatgt tccggcgcga gcggccgcag cgcggccgct accgccagtt tcaccagatc
    17761 ggcgccgagg cgctgggctt cggcggcccc gaagtggacg ccgaactgat tctgctggcc
    17821 gtggcgctgt ggcgcgagct gggcctgaca ggctggcggc tggagctcaa cagcctgggt
    17881 cagcccgacg agcggcgcga gcaccgcgcg gcgctggtgg cctacctgca agcccaccac
    17941 gaccagctcg acgaggacgc gcgccggcgc ctgcacagca acccgctgcg catcctcgac
    18001 accaagaacc cggccatgca ggcgctggtc gagggcgcgc ccaagctgct ggactacctg
    18061 ggcgaggcct cgcgccggca tttcgccgcc gtacaggcca tcctggcggc cgccggggtg
    18121 tcgtggcaca tcaacccgcg cctggtgcgt ggcctcgact actacaacct gacggtgttc
    18181 gagttcgtca ccgaccgtct gggcgcgcaa ggcaccatct gcggcggcgg ccgctacgac
    18241 tacctgatcg agcagattgg cggcaagccg gcgccggccg tgggctgggc gctgggcgtc
    18301 gagcgcgtgc tggaactgct gcaggaagag ggcgtgcaca cgctggagcc ggtgcccgac
    18361 gcctacgcca tcatccccga cgccgcggcg ctgccgcagg ccatgcgctg cctgcagcag
    18421 ctgcgcgcgc tcggcgtggc ggtgcagatg cacgccggca gcggcgaagg catgggcagc
    18481 atgaagagcc agttcaagcg cgccgacgcc tccggcgcgc gccacgcact gatctttggt
    18541 gccgatgagc tggccgccgg cgaagtcacc gtcaaggcgc tgcgcgacgg cgccggtgcc
    18601 cagctgcgcc agccgctggc acagccggac gcctgggccg acatcctaaa atcgaccgct
    18661 taaacaccga accaccacgc atggccaccc atctcgacct cgaagagcag gaacagctcg
    18721 acgccctcaa gcatttctgg aaccgctggg gcaacctcat cacctgggtg ctgatcgccg
    18781 tgctgggcgc ctatgcggcc tggaacggct ggcagtactg ggagcggcgc caggcgggtc
    18841 tggcggcggt gctgttcgac gaggtcgagc gcgccgccca ggccggcgac gcggcgcgcg
    18901 tggagcgcgc gctggccgac atcaaggaca aattcggcgg caccacctac gccgcccacg
    18961 ctggcctgcg cggggccagg ttcttcgacg aacagggcaa gaccgccgaa accaaggcca
    19021 ccctggcctg ggtggccgag aaggcgccca acgacgggct gcgcgccgtg gcgcgcctgc
    19081 gcctggcctc ggtccagctg ggcgagaagg cctacgacga ggcgctcagg acgctggagg
    19141 ccggcttccc gccgtcgttc gtcgcgctgg cggccgatcg ccgcggcgac gtgctgctgc
    19201 agcagggcaa gcgcgagctg gcggcggccg aattcggcaa ggcctaccag ggcatggcgg
    19261 ccgacaccgc gggcgactac cggcgcctgg tcggcatcaa gctgaacgcg ctgggcatcg
    19321 accccgacgc cggcgccaag gcgggagccg cgtcatgacg gcgcgcggta cggtgcggcg
    19381 ccggatcgcc tgcggcgcca gcctgctggc tgcgcttgtg ctgacggcct gcgccggcgg
    19441 ctcaccgcgg ccccagccgg ccgagttgcc ggccaacatc gccctgatcg gcgtgcgcca
    19501 ggcctggacg gcccggctgc cggcgatcga ctttccgctg caggtggcgg tgcagggcca
    19561 gcaggtcacg ctggccgcca acgacggcag cgtcgtcacc ctggacgccg cttccggccg
    19621 cgaactcggt cgcgccagcg ccggtgcgcc gctgacggcg ggtgtgggca gcgatggccg
    19681 cgtgagcgcc gtggtcacgc gcgacaaccg cgtggtggcg ctcgaaggcg gtcgcgaact
    19741 gtggcgccag aagttgggca cacagagcta caccgcgccg ctggtggccg gcgggcgcgt
    19801 cttcgtgctc ggcgccgacc gctcgctgag cgccttcgac ggccgcaacg gcgcccgtct
    19861 ttggcatctg gagcgtccgg ccgagccgct ggtgctgcgc caggccggcg tgctgcaggc
    19921 cgtgggcaac accctggtgg ccgggctggc ggggcggctg gtgggggtcg atcccgacag
    19981 cggcagcgtg cgctgggagg cgccgatcgc ctcggcacgt ggcaccaacg acgtcgagcg
    20041 cctgatcgac ctggtgggcg gcgtcagccg ccacgagggc gtggtgtgcg cgcgggcctt
    20101 ccagacggcg gtcggctgtg tcgacaccgc cagtggccgc ctgcgctgga gcaagccggc
    20161 cagcggcgcc accggcgtgg ccggtgacgg tcagctgctg ttcggtgccg aagccgacgg
    20221 ccgcgtgctg gcctggcgtg ccgacaccgg cgaccgcgcc tgggtcaacg aatccctgca
    20281 gtggcgccgg ctgacggcgc cgctggtgct tggccgctcg gtggtggtgg gcgacggcaa
    20341 cggcctgctg catttcctgt cgcgtcagga cggcacgccg ctggatcgcc tgagcaccga
    20401 cggtaccggc atcgccgcca ccccggtggc ggccggcaac acgctggtgg tggccacgcg
    20461 cggcggtacg gtgtatggct tcgtgcccga atgacatggg cgccgctgca acctgcctgc
    20521 gccgccggac catgctggcg gcgcctgcgg acgccgccat ggccggcatg agccgcgccg
    20581 ccgccacggg ctggtgcggc aggccgcgct ccggccccgg atcgacacca tgaaacccgt
    20641 cattgcgctg gtggggcggc ccaatgtggg caaatcgacg ctgttcaacc ggctcacccg
    20701 cagccgcgat gccatcgtgg ccgacttcgc cgggctgacg cgcgaccggc attacggtca
    20761 gggccacctg ggcaaacgcg aatacatcgt catcgacacc ggcggcttcg agccgcgggc
    20821 cgagagcggc atctacaagg aaatggccaa gcagacgcgc caggcggtgg ccgaggccga
    20881 cgtggtgctg tttctggtcg acgcccgggc cggcctgtcg gcgcaggacc acgacatcgc
    20941 cgactacctg cgccgcctgg gcaagccctg cctgctggtc gccaacaagg ccgagggcat
    21001 gcgcgagggg gcgcagctgg ccgagttcta cgagctgggc ctgggcgcgg tgattccggt
    21061 gtcggccgcg cacggccagg gcgtgcgcag cctgctggaa ggggcgctgg atgcgctcga
    21121 tctgcccgac gtcgacgaag cgccacccga cgctgccgat ggcgtgatcc gcctggcggt
    21181 ggcgggccgc cccaacgtgg gcaagtccac cctcatcaac acctggctgg gcgaagagcg
    21241 cctggtggcg ttcgacctgc ccggcaccac gcgcgacgcc atcagcgtgc cgttcgagcg
    21301 cgccggtcgc cagttcgagc tgatcgacac cgccgggctg cgtcgcaagg gcaaggtgtt
    21361 cgaggcgatc gaaaaattct cggtggtcaa gacgttgcag gccatcgaat cggcccatgt
    21421 ggtgctgctg ctgctggacg cgacccaggg tgtgaccgac caggatgccc acattgccgg
    21481 cttcatcctc gaagccgggc gcgccgtggt gctggcgatc aacaagtggg acgcggtgga
    21541 cgactaccag cgcgagctgg tgcggcgctc ggtggaatcg cgcctgggct tcctgaagtt
    21601 cgccgagctg cacttcatct ctgtgcgcaa gcgccagggc ctggggccgc tgtgggcctc
    21661 gatcgcgcgc gcccaccagg ccgccatgcg caagctgccg acaccgcagc tcacccgcac
    21721 cctgctggag gcggtgcagt tccaggcgcc caagcgcgcc ggcatgtacc ggcccaagct
    21781 gcgctacgcc caccagggcg gcatgaaccc gccggtgatc gtggtgcacg gcaactcgct
    21841 cgaaggcgtg accgacacct acaagcgcta cctggagggg cgctttcgac aggtgttcga
    21901 cctcatcggc acgccgctgc gcatcgagat gaagaccacc cacaacccct acgccacaag
    21961 gcctgaccag gggtgaacgg ccccccatgc cggcttggac cggcctgcca cggccgctcg
    22021 gcggcgcctt ttctacccgg tttacccctt gtgctgggcg gcaccacccc catgtggtaa
    22081 gttccatctc tcttcttaca ctttccgaac acggagaata tcgtgagcaa caaagggcag
    22141 cttctacaag acccgtttct gaacaccctg cgccgtgagc acatcccggt ctccatctat
    22201 ctggtgaacg gcatcaagct ccagggtcag atcgaatcct tcgaccagta cgtggtgctg
    22261 ttgcgcaaca acgtcaccca gatggtctac aagcacgcga tctccaccat cgtgcccggc
    22321 cgtccggtca atttctcggc caccggggaa gtcgcggaat cggattgatt ttggcctcca
    22381 gcgcccaccc ggcgtgcgcc agcagctatt gattcaatag caatctggcg tttctgacaa
    22441 ggcgctttgc cactttgccc cgtaccgcca cccctgaacg cgctgccgcc atcctggtcg
    22501 gggtggatct gggggtgccc catttcgacg gccaactgca ggagctgggc ctgctggccg
    22561 aaaccgccgg cctgcagccg gtggcgcgcc tgacctgcaa gcgccaggcg cccgatgcgg
    22621 cgctgttcgt cggccgcggc aaggtcgagg agatccgcct gctggcgcaa agcgagggcg
    22681 cgcgcgaagt gctgttcgac caggcgctct cgccggtgca gcagcgcaac ctggagcgcg
    22741 ccatcggcct gccggtcaac gaccgcaccc tgctgatcct ggaaatcttt gcccagcgcg
    22801 cgcgcagcca cgaaggcaag ctgcaggtcg agctggcgcg cctgcagtac ctcagcaccc
    22861 gtctggtgcg ccgctggtcg cacctggagc gccagcaggg cggcatcggc gggcgcggcg
    22921 gcccgggcga aacccagatc gaactggacc gccgcatgat cggtgaagcc atcaagcgca
    22981 ccaaggatcg cctgcagaag gtcaagcgcc agcgccagac tcagcggcgc cagcgcgagc
    23041 ggcgccacac cttcaacatc tcgctggtcg gctacaccaa cgccggcaag tcgacgctgt
    23101 tcaacgccct ggtgaaggcg cgcgcctatg ccgccgacca gctgtttgcc acgctcgaca
    23161 ccaccacgcg ccagctctac ctgggccagg ccgggcgttc ggtatcgctg tcggacacgg
    23221 tgggcttcat ccgcgacctg ccgcacggcc tgatcgaggc cttcgaggcc acgctgcagg
    23281 aggcggccga cgccgacctg ctgctgcacg tggtcgacgc cgccaacccc ggtcacctgg
    23341 agcagatcga ggaggtgcag cgcgtgctgc gcgaaatcgg cgccgccgag ctgccgcagc
    23401 tgctggtgtt caacaagctc gacgagctgg agccggcgcg ccgcccgctg cagctcaagg
    23461 acagcgtgac gctggacggc cgcgagctga cgcgcatttt cgtcagcgcg cgcagtggcg
    23521 aaggcctgcc gctgctgcgt gccgaactgg aggcggccgt gcagagccag gcgctggaag
    23581 cgggcggcga cggggtgccg gcgccgattg ggcacaatga tgggttgcac agccgagaag
    23641 tcgacgcatg aatgaccgca ccagtgcccc gcgaccggcc tcatggccgg gccggatccg
    23701 gggcatgttc aacctccatg acccgcgctg ggggcgcgac gacgaaccgt cggggcagga
    23761 gccgggccag cgcccggacg agccccagcg gccaccgccg cgtggccagg gcccgcaggc
    23821 cggaccgccc gacctcgacg agctgtggcg cgacttcaac cgcaagctgg gcggcctgtt
    23881 cggcggcggg cgcgggcggg gtcgcaaccc gtactcgggt ggcggcatgc cgcccgagat
    23941 gaagggccgc ggcgccggca tcggcatcgg cgtgatcgcc gccgtggcgc tggcgatctg
    24001 gctggcctcg ggcgtgttca tcgtgcgcga aggcgagcag gccgtggtca ccaccttcgg
    24061 tcgcttcgac ggcatccgca aggccggcct gaactggcgc atgccgtacc cgatccagcg
    24121 gcacgagacg gtgcgggtga cgcagatccg ctcggtcgac gtggggcgcg acagcatcat
    24181 ccgcagcacc ggtctgcgcg agtcggccat gctgacgcag gacgagaaca tcgtcgagat
    24241 caagttcgcc gtgcagtacc gcctgaacga cgcgcgcgcc tacctgttcg aaagcaagag
    24301 cccggacgac gccgtggtgc aggccgccga aagcgcggtg cgcgaagtgg tcggcaagat
    24361 gaccatggac accgccatgg ccgaggagcg cgaccagatc gccccgcgcg tgcgcgacct
    24421 gatgcaggcc atcctcgacc gctaccaggt gggcatcgag gtggtggccg tcaacatgca
    24481 gcagggcggg gtgcgtccgc ccgagcaggt gcaggcggcg ttcgacgacg tgctcaaggc
    24541 cggccaggag cgcgagcgcg tcaagaacga ggcccaggcc tacgccaacg acgtggtgcc
    24601 gcgtgccgtc ggtaccgccg cccgcctgaa gcaggaggcc gaaggctaca aggcccgcat
    24661 cgtggcgcag gcgcagggcg atgcgcagcg cttctcgtcg gtgctgagcg aataccagaa
    24721 ggcgccgcag gtcacgcgcg agcgcatgta cctcgacgcc atgcagcaga tctacggcgg
    24781 cgtcaacaag atcatcatcg acagccgcgc cggcggcaac ctgatgtacc tgccgctgga
    24841 caagctgctg cagggtgcag caggcgcggg cgcggcggcc ggcgcggcgc cggccgccgc
    24901 ccaggcaccg gcgccggcca ccgccccggc tgcgcccgcc gccgacccgc gcgcgcgcga
    24961 caacgcccgc acgcgcgagc gtgaaacccg ctaagtcagg ccgcacccca tgaacagagt
    25021 cggattcatc ggaaccaccg tcctcatcct gctggccctg ctgggctcga ccgtgttcgt
    25081 ggtcgaccag cgccagttcg gcgtggtcta ccagctcggt cagatccagc gcgtcatcac
    25141 ccagcccggg ctgaatttca agctgccgcc gccgatgcag aacgtcacct acatcgacaa
    25201 gcgcctgctc acgctcgaca gcatggacac cgaacccatg ctgacggccg aaaagcagcg
    25261 cgtggtgatc gactggtacg tgcgctggcg catcaccgac ccgcaggcct acatccgcaa
    25321 cgtcggcctg gacgaaagcg ccggcgccct gcagctcaac cgcgtggtgc gcaacgcctt
    25381 ccaggaagaa atcaaccgcc gcaccgtgcg cgagctgatc tcgaccaagc gcgaaaccct
    25441 gatggccgac gtcaagcgtg aagtgtcgga cgccgtcaag ggcgccaagc cctggggcat
    25501 cgacatcatc gacgtgcgca tcacgcgcgc cgactacgtg gaatcgatca ccgagtcggt
    25561 ctaccgccgc atgcaggccg agcgccagcg cgtggccaac gaactgcgct ccaccggcgc
    25621 cgccgagggc gagcagatcc gtgccaacgc cgaccgccag cgcgaagtga ccgtggccga
    25681 cgcctaccgc gaagccgaga aggtgcgtgg cgagggcgac gcccaggcca cccgcatcta
    25741 tggcgacgcc ttcggccgcg acccgcagtt cgccgagttc taccgcagcc tggacgccta
    25801 caaggccagc atcggcaaga agggcgacgt gctggtgatc gatccggcct cgtcggactt
    25861 cttccgcaac ctgcgcggcg caccggcgcg gcgctgacac ccgggggtcc gacggtggac
    25921 ggtgccacct ggctggccgc gctcggcctg gccctggtcc tggagggcct ggtgccgttt
    25981 ctggccccgc gcgcctggcg tcaggccttc agccagttgc tgcaactgcg cgacggccag
    26041 gtgcgcttct tcggtctgct ggcgctggcg ggcggtctgg ccctgctgtg gctggcgtga
    26101 cggcgggccg cgcgcagcca cgccggtaga atcgcctttt caacagccga cgcgaaagct
    26161 gccccagcca tgtccgcctg gctcctgccg gaccacatcg ccgatgttct gccctccgag
    26221 gcccggcaca tcgaagaact ccgccgcgaa ctgctcgaca ccgcccgtgg ctatggctac
    26281 gagctggtga tcccgccgct gatcgaacac ctggaatcgc tgctcaccgg caccggcgaa
    26341 gcgctgggcc tgcagacctt caagctggtc gaccagctgt cgggccgctc gctgggcgtg
    26401 cgcgccgaca ccacgccgca ggtggcacgc atcgacgccc atctgctggg ccgtcaggcc
    26461 gtcacgcggc tgtgctactg cggcccggtg ctgcacaccc agccggagcg cccgcacgcc
    26521 acgcgcgagc cgctgcagct gggcgccgaa atctacggcc acgccggccg cgaagccgac
    26581 ctcgaaatcc tgtcgctggc gctggactgc ctgcgcgccg cgccggtcca tggcctgtcg
    26641 gtcgacctgg ccgacgcgcg catcgtgcgc gccctgctgg ccggcgtgct ggtcgacccc
    26701 gagcgcctga ccgaggtgca tctggcgctg gccgccaagg acgccagcga actggcccgg
    26761 ctgaccgcgg cgtttcccgc cagcgcgcgc cagggcctgt gcgcgctggt cggcctgtat
    26821 ggcgatgcgg gtgtgctgga tgaggccgaa aaagccctct cgggcgcacc tggcgtgcgc
    26881 gaggtgctat ccaatctgaa gtggctggcg agccagctgg agggtgtgcg ggtcagcttc
    26941 gatctggccg acgcgcgcgg ctggggctac tacaccgggg cccgctttgc cgtctatgcc
    27001 gaaggggcgg gcgacgcgct gctgcgcggc ggccgctacg acggcgtagg cgcggtgttc
    27061 ggccacaagc tggaccgcga acggccggct gtcggcttca gcctcgacct caagcaactg
    27121 gtgggcgtgg ccgcgccgcg cccgctgcgt gccgccatcc gcgccccctg gggcgatgcc
    27181 ccgggcttgc gcaaggcggt ggcggggctg cgccggcgcg gcgaaaccgt ggtctgcgcc
    27241 ctgccgggcc acgccgacca gatcgacgaa tttcactgcg accgcgagct catcgaggcc
    27301 ggcgggcagt gggtgatcca ggcgctcgga tgagcccgcc tgcccgatcc gctgcgcgcc
    27361 gccacggcgg tgcgcgccgg gccacgccgc tggccggccg gcccgatccc gacccccgaa
    27421 tccacagcaa tcaaggcaaa gcatgaaacc ctccaccacc ggacgcaacg tcgtcgtcgt
    27481 cggcacccag tggggcgacg aaggcaaggg caagctggtc gactggctga ccgagagcgc
    27541 gcagggcgtg gtgcgcttcc agggcggcca caacgccggc cacaccctgg tcatcaacgg
    27601 cgtcaagacc gcgctgcacc tgatccccag cggcatcatg cgcccgggcg tgacctgcta
    27661 catcggcaac ggcgtggtgc tgtccgtcgc caagctgctg gaagaaatcg ccggcctgga
    27721 gcgcgccggc gtgcaggtgc gcgagcgcct gcgcatcagc gaaggctgcc cgctgatcct
    27781 gccgctgcac gccgccatgg acgtggcgcg cgaagccgcg cgcgagcagg gcggcagcga
    27841 gaagatcggc accaccggcc gcggcatcgg tccggcctac gaggacaaga tcgcccgccg
    27901 cgccctgcgc gcacaggacc tgaaacaccc cgagcgcttc gccgtcaagc tgcgcgagct
    27961 gctggcgctg tacaaccatg agctgctggg ctatctgggt tcggcgcgca tcgccttcaa
    28021 cgaggcgctg acgccctacc tgaaagacgg cgcactgcag ttcgagccca tctacgacga
    28081 ggccatgcgc caggccgagc aggtgcgccc catgctggcc gacgtctcgc gcgagctgaa
    28141 cgaggcccac cagcagggcg ccaacctgct gttcgaaggc gcccagggca cgctgctgga
    28201 cgtcgaccac ggcacctacc cgttcgtcac cagcagcaac accgtggccg gcaacgccgc
    28261 cgccggcgcc ggcgtcggcc cgggcctgct gcactacgtg ctgggcatca ccaaggccta
    28321 ctgcacgcgc gtcggcggcg ggccgttccc gaccgagctg gactggcaga cccccggcac
    28381 ccccggctgg cacatgagca ccgtcggcgc cgaaaaaggc gtcaccaccg ggcgcagccg
    28441 gcgctgcggc tggttcgacg ccgcgctgct caagcgctcg gcccaggtca acggcctgtc
    28501 gggcctgtgc atcaccaagc tggacgtgct cgacggtctg cccgagctgc agctgtgcac
    28561 cggctacgaa ctggatggcg agacgatcga catcctgccg ctgggcgccg acgacatcgc
    28621 gcgctgcaag ccgatctacg aaaccatgcc cggctggagc gaaagcacgg tcggcgccac
    28681 ccgctacgag cagctgccga tccacgcgcg gctgtacctg cagcgcatcg agcatgtcac
    28741 cggggtgccg atccacgtcg tttccaccag ccccgatcgc gaccacacga tcatgctgcg
    28801 gcaccccttc ctgcctacct gatacaggcc aaatcacctt gcagcgcccg ccagaacggc
    28861 gccaccagct atcaaaagag gagagacccg catgttgacc gaagacggca aacacttgta
    28921 cgtgagctac gacgagtacc acgccctgat cgagaagctg gcgctcaagg tgcaccagtc
    28981 gggctggcgg ttcgacacca tcctgtgtct ggcgcgcggc ggcatgcgcc cgggcgacat
    29041 cctgagccgc atcttcgacg tgccgctggc catcatgtcc accagcagct accgcgccga
    29101 ggccggcaag gtgcagggcc atctggacat cgcgcactac atcaccacgc cccagggcca
    29161 gatcgccggt cgggtgctgc tggtggacga cctggccgac accggcgtca cgctgcaggc
    29221 ggtggtcgag cgcctgcgca acgactaccc gcccatcacc gaattgcgca ccgccgtgat
    29281 ctggaccaag ggcagctcca gctttcaggc cgactactcg gtcgagcacc tgcccaccaa
    29341 cccctggatc caccagccct tcgagagcta cgacggcctg acgcccgaca agctgctggc
    29401 caagtggaag gtgtgagctg caagctgtga ggcgcgctcg gccgctgccc cggcctttgc
    29461 gctgagccgc acgggctgcc gcgcgccggg ggcggtcgcg gtcgccgatc gcctaccatc
    29521 gccccatgac cgaccccatc acccagctga tccaccaccc ctaccagccg ccggccggct
    29581 ttgcggcgcc gcagccggcc gtgcacaagg cctcgacggt gttcttcccg aacgtggccg
    29641 ccatgcgcga gcgcgactgg aagcacaaga gcggctacac ctacggcctg cacggcacgc
    29701 ccaccacctt cctgctggaa gagcgcatcg ccaccctgga aggggggctg cagtgcgtgc
    29761 tggtgcccag tggcctggcc gccatcgcca acgtggcgct ggcgctgctg cagaccggcg
    29821 acgaggtgtt gatccccgac aacgcctacg gccccaacaa ggcgctggcc gagggcgaac
    29881 tggcggcctg gggcatcacg caccagtact acgacccgat ggatctggcc gatctggcgg
    29941 cgcgcatcgg cgagcgcaca cgcctggtgt ggctggaagc ggccggctcg ctgacgctgg
    30001 agtttcccga cctgatcggg caggtgcgcc tgtgccgcgc gcgtggcgtg ccgtgcgcgc
    30061 tggacaacac ctggggcgcc ggcctggcct tcaacccgtt cgatctggac ggtcagggcc
    30121 tgggggtgga cctgagcgcg cacgcgctga ccaagtaccc cagcggcggc ggcgacgtgc
    30181 tgatgggcag cgtcaccacg cgcgacgagg cgctgcacct gaagctcaag ctgacccaca
    30241 tgcggctggg cctgggcgtg gccgccaacg acgccgaagc ggtgctgcgc gccctgccca
    30301 gcgtggccct gcgctaccgg gcgcaggacc aggccgcgcg ggcactggcc ggctggtgcg
    30361 cgcagcagtc ggcgttcgcc caggtgctgc acccggcgct ggccgatgcg cccggccatg
    30421 cccactggca gcaggtgtgc ggcgccgccg acgctcgcgg gcagggcgcg gcggccggcc
    30481 tgttcagcgc gatcgtgcac gagcgctaca gccaggccca ggtcgacgcc ttctgcgaca
    30541 cgctcaagcg gttcaagctc ggctacagct ggggcgggcc gatcagcctg gtggtgccct
    30601 acgacctggc ggccctgcgc gcgcgcggct ggccggcgca cctcaggcgc ggcacgctgg
    30661 tgcgcttttc caccggcctg gaggcgctgg ccgacctgca ggccgatctg cagcaggcgc
    30721 tgaaccgcgc cctgccgtcc tgaagctata gtttatgtag ctgttggcgc ttgttgggcg
    30781 ggcgccagag tgcgatttga tgctgcgtcg ccggtgtcaa ccaggcgccc gggtcattac
    30841 ccattgagtg ggcatggttt tttggctatc ttgccgcccg agcaccgccc ttcggcggtc
    30901 acccggtcac ccatgagcca gcccgacacc gatcccaccg ccgccccgcc ggccgccgcc
    30961 ccggcgccgg cctatcagcc ctacgtgccg cccccggtca ccaccccgca ggccatcatg
    31021 gtgccgctgg gcctggccga cccgctgcgc tggctggggc gcggctggcg cgacttcatg
    31081 gccgcgccgg gcatcggcct gttctacggc gtgtgcttct ggctgatggc ggcgctgctg
    31141 gcgctggtgt ttcgcagcaa gcccgaatac gtgatgtcga tcgcctccgg ctgtctgctg
    31201 gtggggcctt tcctggccat ggggctgtac gaggtcagcc gccggcgcga gcagggcctg
    31261 gtgcccgagc tggccgcctc gctgacctgc tgggaccggc acgtgcgcag catgggcatg
    31321 ctggtgctgg tgctgatcgt gctggagctg ctgtggggcc gcgcctcgct ggtggtgttc
    31381 gcggtgttct tcaacaccgg catgccgtcc accaccggcg tgctgcaggc catcttcaac
    31441 cccgacaacc tcgaattcgt gctggtctat ctgacggtgg gcggcttctt cgcgctgctg
    31501 gtgttttcca ccacggtggt gtcgatcccc atgatcctcg accgcgacac cgacgccatc
    31561 agcgccgcca tcaccagcat ccgcgtggtg ttcgccaacc cgttgacgct gctgtggtgg
    31621 ggcctgctga tcaccttgct gatcgcgctg tcgctgctca gcctgggtct gggcctgctg
    31681 ctggtggggc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgctgatcg
    31801 cgctgtcgct gctcagcctg ggtctgggcc tgctgctggt ggggccgctg ctggggcacg
    31861 ccagctggca tgcctaccgc ggcgccgtgc agtggcgcga gccgcccccg cgcccggccg
    31921 acgcctgcgt gccggccccg gtgccggcgc cggcgccgct gcccggctga acgccgtcag
    31981 gcggggtggc cggcgcctgg ctggccagga tcagcgcgca ggcggcgcgc aggctggcgg
    32041 gcgagcggtc ggccagcggc accgccaggg cctggccaaa gcgcgcgaag ttgcgctgcg
    32101 tcgagctggc gtagcccggg tcgtagtgcc gggccagcag ctcacgcacc acctgatcgg
    32161 tgtggccggc ggccacgcgc gccttccagt cgtcgatcac ggcgcgcccg cgcagcgcca
    32221 ccagggtgtc caggcgctgg cagaagaaat ccgggtcgcg gacgaagaag gggtagtcct
    32281 ccagcagcag cgccacccgc tcgtcgtcgg ccagctcgat gcgcaggcag tcgctggcgc
    32341 gcatggcggc gatcagcgcc tcgggcacgg tgacgttgcc gaccttcttg ctctcggcct
    32401 ccacgtacac cggtcgcgcc ggctccaggt ggcgcagcgc gtcccacacc cgcatctcga
    32461 agtgcttctg cgacggctgc ggctggcccg gaatcagccc cagcaccgag gcgcggtgct
    32521 gcgccagccc ttccaggtcc agcacctgcg cgccggccgc tgccagcgcg tgcagcaggc
    32581 gcgtcttgcc cgagccggtg gggccgcaca ccacgcgcag gcgcaggcgc tgcacctgcg
    32641 ccggaatgtc ggtcaggagg gcgacccgga aagctttgta gccgccttcg atcagcgtca
    32701 cctgaaagcc gatctgtccc agcaccagcg ccagcgcgcc gctgcgctga ccgccgcgcc
    32761 agcagtacag cagcgggcgc cagcggcgcg aggtgtgggc cagctcgcgc tcgatgtggt
    32821 cggcgatgtt gcgcgccacc agggcggcgc ccagcttgcg cgcctcgaac gggcttgcct
    32881 gcttgtacag cgtgcccacg cgggcgcgct gctggtcgtt caggctgggc cagttgacgg
    32941 cgcccggcag gtggtcgtgc gcgaattcgc tttcgctgcg ggcgtcgatg atggcgctga
    33001 actcggccag tcgcgccagg gcgtcggcgg cggcgatggt ctggacgctc atggtttcag
    33061 cagccggcgc agctcgggcc agacgttgtc gaggatgatc gggtgcgccg ccgccgtggg
    33121 gtggatgcgg tcgggctgga acagcgcttg ggcgtggtcg ccatcggcca cgcccttgag
    33181 cagaaacggc accagcgccg cccgctgcgc cttggccacc tgcgggtaga gcgcgaagaa
    33241 gcgccgcccg aagtcggcgc cgtagttggg cggcatttgc atgccggcca gcagcacctt
    33301 ggcgccggcg gcctgcgccg cctgggtcat ggcggtcagg ttggcctcgg tcatctccag
    33361 cgacagcccg cgcagcgcgt cgttgccgcc cagctctatc accacgacgg ccggccggtg
    33421 cgccttcagc agcgccggca ggcgcgagcg cccgcccgcc gtggtgtcgc ccgagacgct
    33481 ggcgttcagc acgcgggcgg cgatctgctc gcgcgccagg cgttgctcca gcagcgccac
    33541 ccagccgctg ccgcgggcga tgccgtattc ggcgctgatc gagtcgccca gcaccaggat
    33601 caggggcggc gcggccgtct gcgcggccag gcccgccggc gcggcggcca gcaggaagcg
    33661 gatacagtct cgacgattca aatcggatgc cttttcatga cggatgtttc gcacggggcg
    33721 ccagcgggcg cccgccgcgc tcccatcatc gcggtggagc acgtgttcaa gtcggtcacc
    33781 gactccagcg gcacgctgga tattctgcgc gatatccact tcagcctggc cgagcgcgag
    33841 acggtcgcca ttgtcggcgc ctccggctcg ggcaagagca ccctgctgtc gatcatcgcc
    33901 gggctggaca cgcccacccg cggcacggtg cgcattgccg gcgacgacct gtttgccgtc
    33961 tccgaagacg cgcgcgcggc gctgcgggcg cgtcgcatcg gcttcgtgtt ccagagcttt
    34021 cagctgctgg gcaacctgac ggcgctggaa aacgtgatgc tgccgctgga gctggccggc
    34081 cggcgcgacg cccgcgccgc cgccaccgag atgctggcgc gcgtgggcct ggccgagcgc
    34141 ctgcgccact accccaaggt gctgtcgggc ggtgagcagc agcgcgtggc gctggcgcgc
    34201 gccttcgtgc tgcacccggc ggtgctgctg gccgacgagc ccaccggcag cctcgaccac
    34261 gccaccggcg agaaggtgat ggcgctgatg ttcgagctca accgcgagca gggcaccacc
    34321 ctggtgctgg tcacgcacga ccgcggcatc gcggcgcgct gccagcgccg gctgaccatc
    34381 gacgccggtt gcatcgcggc cgacgagcaa gctgaatcgc tatcgataaa gtagctgctg
    34441 gcgctcacca gacgggcgct ggagcctgat tttccttaga gctggaaggc gagggcgccg
    34501 accacgcctt ccagcacgtc gcgcagcagg ccgggcggcc gggcggaggc cggctcgccg
    34561 tcggcggcgg tgcgggtgat ccagccggcc agccagtcga tctcggcgcg gccgtagcag
    34621 gccgccatgg cttcgtagtt gatgaacagg ctgcggctgt ccaggttggc cgagccgcac
    34681 agcgccagcg cctggtccac caccaccgcc ttggcatgca gcatggtggg cagcaggcgc
    34741 acctcgacgc cgtgctccac cagcgcgcgc accgcgcgtc cgcgcgccca gtcggccagc
    34801 cggtggttgg agcggcgcgg cagcagcagc gtcacgcgca cgccgcgctt ggccgccagc
    34861 agcagcgctt ccagcagccc ctcgtccggc acgaaatagg gcgtggccag cagcaggcgc
    34921 tgctgcgcat ggaaggcggc cgacaccagc agcgcgtgca gcacgtcctc atggaaatcc
    34981 ggcccgctgg gcacccagtg cgccagcgcc acctgggccg acggcgctgc gccgctggcg
    35041 gcagggggcg cgctggccag cgcggcggcg ctggcgtcgg ccggctggta ggcgtgcagc
    35101 agcgcctcgt ggcgcagcgc gcgctcggca tagggcacgc ccgaggcgcg ccgcagcccc
    35161 agggcgatgc gccagtcgcc ttcgaacagc gccatggcct gcgtcgccag ggcgccctcg
    35221 gcgctgaagc tgaggtcgat ccagggcgcc tcgcccgccc ggccgatgaa gtattcgtcg
    35281 gccaggttgc gcccgccgcc ccacagacgc aggccgtcgg ccaccaccag cttgcggtgg
    35341 ttgcgcaggt tggtgcggcc gcgccgcgga ttgcgcagcg ccggcatgaa gcggcgcacg
    35401 cgcaccccgg cggcgcgcag ccgggcgtcg tggctgcgcc ggctcctgag gctgccgacg
    35461 ctgtcgacca gcaggcgcac gcgcacgccg gcgcgggcgc gcgcggccag ctcgtcggcc
    35521 accgccgcgc ctacctggtc gtcgcccagc acataggtgc acacgtccag cgtgcgttcg
    35581 gccgacgcga tgagcgcgcg cagctcggcc agcgcctgcg cgccatcggc atgaaagcgc
    35641 agcgccgcct gcggccgcgc ctcgggcacg cccagcgccg ccagcaggcc ggtggcccag
    35701 gccggcgcca gccgcgccca ggggccggcc ggcaccggct ggcgcggggt gctcgggcgc
    35761 accgtcttgc gcgtgccgaa cagcagaaac agcggcaccc ccagataggg aaaggccgca
    35821 atccccagca cccaggccag cgccgcgtag gggtggcgcc gctgctgacg cgcccgcgtc
    35881 accagcacgt acagcagcag gccggcggcc acgaaggcgg catgttccag cggactgagc
    35941 gtgggcagac ggatcatgcg aaacgttcag gggcttcggg ttgctcttga aaacatagcg
    36001 tgcggcgccc ggcaggtacg gccggcaggc cgcaatcggt ctgattatgc ggcgcggcgc
    36061 gtgggatcgg cgcggtggga tgccccgccc gggccgctcg catgctattg aataactagc
    36121 tgctggcgct ttgccggtgc gcgccgcagc cccaaaacgc tcaccaaccg ggggcgccgc
    36181 cgggcaccga taatcacccc atgtccaccc ccaaagtgct gttcggcttc catgccgtcg
    36241 gcgtgcgtct gaagaccgcg cccgcctccg tcatcgaggt ctatgtcgat cccggccggc
    36301 gcgacgcgcg catgaagcag tttctggcgc gtgcggccga agccggcgtg cgcgtgatcg
    36361 aggccgatgg cccgcgcctg gcccggctgt gcggcggcgc cggccaccag ggcgtggtgg
    36421 cgcgcgtgca gccgatcgcg cagacgcatt cgctggacga actgctggac ggcgtggccg
    36481 agccgccact gctgctggtg ctcgacggcg tgaccgaccc gcacaacctg ggcgcctgtc
    36541 tgcgcgtggc cgatggcgcc ggcgcgcacg ccgtcatcgc gcccaaggac cacgccgccg
    36601 gcatcaacgc caccgtcgcc aaggtggcca gtggggcggc cgaaaccgtg ccctacctga
    36661 tggtcaccaa cctggcgcgc acgctgggcg agctcaagga gcgcggcatc tgggtcatcg
    36721 gcacctcgga cgacgcgccg cagaccctgt accaggccga cctgaagggc ccgaccgctc
    36781 tggtgctggg cgccgaaggc cccgggatgc gccagctcac gcgcaagagc tgcgacctgc
    36841 tggtgcgcat tcccatgcac ggcgcggtgg agagcctgaa cgtctcggtg gccagcgcgg
    36901 tctgcctgta tgaggcgcgg cgccagcggg gctgaagcgg cttcgggttc aagattcgtg
    36961 acccatgacc ccatgaccga tggaccgtgg gtcattgaac ctagaaacag cagttgaccg
    37021 cttgccaact tcgggcagac cgcatcgatg cgcggtctgg cggctgcgat ggccttcacc
    37081 gcctgggtga gtccgctgcg cacccacccg caccgcgctc ggcgcgccat gcggcgttgc
    37141 tcctgcttgc gggcacgagc ccgcggcgtc gtcgcgtctt gcctgccacg ccgagcgcgg
    37201 cacgggcggg cgcgtccaac tgccgtttct aggttgaacc caggttgaag cgcagcgagg
    37261 tcatccttca tgtcgaaagc gagttcgtgc gagccgtcgc tgaagtacga atcgtaacta
    37321 cgcacaaacc cgatgcggga agctgccgat gcacgcccgc agggcgctcg gctaccgtcg
    37381 ggcgatcatg ccgtcatcgc tggtcgcccg tcccgccgaa ccctcgcgcc gccttttgct
    37441 gcggctggcg gcggccggcg cgctcggcct ggctggcgcg gcggcggcac agcggccgac
    37501 gcccgagcgc accagcgtga cgctggcggt gggcggcaag acctcgctgc cctgtctgcc
    37561 gctgaccatc gccgagcagc tgggctactt tcgtgacgag ggcctgcagg tcgaactgca
    37621 ggaccacccc ggcggcggcc tggcgcagca ggcgctgctg ctggggcgtg ccgacgtcgc
    37681 ggccgggggg ttcgagcacc cggtgctgct gcgcccgcgc ggcacccact gccgcgcctt
    37741 cgtgctgctg gggcgcgcgc cgcaactggt gttcggtgtc ggcacgcggg cgctgcccga
    37801 gtttcgttcc ctggcccagc tcagggggcg gcgcgtgggc gtcacctcgg ccgactcgtc
    37861 ctcgcactgg ttcgcccagc gcctgctggc gcgcgccggg ctggcgccga ccgatgtcga
    37921 atatgtcggc gtcggcaccg ccatcacgcc ggtagcggcg ctgcgcgaag ggcgcatcga
    37981 cgccctggcc gacatcgacc ccgccatcag cctgctggag taccgcgccg acatccgcgt
    38041 ggtggccgac acgcgctcgc tgcgcggcac gcacgagctg tacggcggcc ccatgccggg
    38101 cgcctgcctg tacgcgcgcg acgccttcgt gctgcgctac ccgcagaccg tgcaggccct
    38161 gaccaacgcg gtggtgcggg cgctcaagtg gctgcacacc gccgggccca gcgacatcgt
    38221 gcgcaccgtg cccgaaagct acatgcacgg cgaccgctcg gtctacctgt cggcgctcga
    38281 caaggcgcgc gaggccctgt cgccggacgg catggccagc gacgacagcg tggccaccgc
    38341 gcaccgcatc gtggcgcagc agctgcaggc caccgacggc ctgcgcgcca ccgcagtggg
    38401 ggcgagccac accaacgact tcgcgcggcg cgcgcggcag cgctttctgg tgtcgtgacg
    38461 cggcgcggcc acgccgccgc ggcgtcggct cagcggcgcg ccttccggcc gcccttgccg
    38521 ggcgccgccg cagcggcctt gccaccgcgc gcggcgcgcg tgggcgcggc ggcctgcttg
    38581 gcgccacccg ccgccctggc cttggccttg gtcttgcccg gcgaccggtc ggcacgtgcc
    38641 gaccgcgggc tgtcggcgct ttcgcgcgcg gcggcgctgc tggcgcgcac cggcaggaac
    38701 atcaccaccg actggcccgg cttcaggcgg gcgccagcct gggtgccgtt ccagccggcg
    38761 acggtggcgg ccggcaggtc gtagcgcgcg gccacgctgg cgatggtgtc acccttgcgt
    38821 gcgcgcaccc gggtgcggcg cagcacgatc tcgggcgcat acgccagctg ggcgttgttc
    38881 accacatggg aggcgactgc ggtggcgctg ccgttggcgc gccgcaccag cacggtggag
    38941 ccgctcttga tcaccacgcc gtgcgggagg ttgttgatct cgcgcaggtc ggactcgtcc
    39001 atgccgaagc ggctggccac ctcgcgcgag ggcaggttct gcggcgccac ccaggcggtc
    39061 cacgagctca gcgtgcccgg atcggccttg gccagcgcct gctgaaacag ggcggcgttg
    39121 ttccagggca gcagcacctg cggcgtgccg gcggcgaaga tcaccggctt gcgctgcgac
    39181 gggttgagcg ccttgaagtc ctccagccgc acctgggcca ggcgggcggc gacgtcgacg
    39241 tcgatgtcgt gcttgatgtc caccgtgtcg aagaaggggt ggttctcgat cagcggcagc
    39301 accgcgccgt ggcgctgcgg ctgggcgatg atgttcttga ccgcctgcag cttgggcacg
    39361 tagttgcgcg tctcggccgg catcttcagg tccaggtagc cggtgcccag gccggcagcc
    39421 tggttgcgtt cgatggcgcg cttgacgttg ccctggcccc agttgtaggc ggccagcgcc
    39481 aggtgccagt cgccgaacat gtcgtgcagc ttctgcaggt agtccagcgc cgcccgggtg
    39541 gagtcgagca cgtcgcgccg gtcgtcgcgc aggatgttct gcgtcagctg gaagtcgcgc
    39601 ccggtgccgg gcatgaactg ccacatgccg gccgccttgg cgctggacac cgcctgcgga
    39661 ttgaaggccg attcgatgta gggcagcagc gccagctcgg tcggcatgcc gcgcagctcc
    39721 agctcctcga ccacgtggaa caggtacagg cgcgagcgct cgaccatgcg ctcgatgtag
    39781 tccgggcgcg tgctgtacca ctgctcgtgg cgctgcacca gctcgtcctg caggtccggc
    39841 atggcgtagc cgcggcggat gcgctcccac agatcggccg gcgtttccag cgccgccacg
    39901 tggttgccgc gcagctcggt gatgcgcacc ggcggcagcg cgtccggcat gcgctgggcg
    39961 ccgcgcggct cggcgcgcgt gggcgtggcg gtgctgacgt cgcgttccgg cgtggcgggg
    40021 ccgggggggg cggcgcaacc ggccagccac aggccggcca gcagcgtcag agagagggtc
    40081 cacttcatcg gaattcgttc ttccattgac gcagggcggc gaacaccgcc accgcgtcgt
    40141 ttgccgaggg gctgtgcgcc cgcaccgccc gggtcaccgc cgcttcgcgg ctgcgcagga
    40201 atgggttgat cgcacgctcc agcccgatcg tcgagggcag ggtcggccgg ccgtcggcgc
    40261 gccgctgcag gcactggcgc tcatggtcgg ccagggcggt gttgtccggc tccacggtgc
    40321 gcgcaaagcg caggttgccc agcgtgtatt cgtgtgcggc gcacacccgg gtggtcggcg
    40381 gcagcgccgc cagcgcatcc agcgaggcca gcagctgcgc cggcgtgcct tcgaaaatgc
    40441 gcccgcagcc acccgaaaac agcgtgtcgc cgcagaacag cagcggcgcg ccatccacgt
    40501 cgcgggcaaa gaaggcaatg tggccggccg tgtggcctgg cacgtccagc acctcgaagt
    40561 ccagccccag cacgcgcagc cggtcaccgc cggccagcgg ggtgaacggc tcggggatgc
    40621 gctcgcgcgc cgggccgtac acggtggcgc cgctggcttc gcgcagcgcg gtcacgccgc
    40681 cggtgtgatc gccgtggtgg tgcgtgacta gaatgcaggc cagccgcagg ccgagccgcc
    40741 tgagcgccgc ctgcaccggc gctgcgtcgc cggggtccac caccagggct tgctggccat
    40801 cgtgcaacaa ccagaggtag ttgtcctcga aggcggggag ggcgatcaga tccatgtggg
    40861 ggcggattat agaaaccgat ggggcggcct tggcgcgcag attcgagcct caccacgtac
    40921 gccggagcat cccgtgaccg gcctgcacca ctggttccag accccgcccg gccagtacct
    40981 gctggcatgg gagcgcgccc agttcgacgc cgcgctggcc gacgtgttcg gctaccacgc
    41041 gctgcaactg ggcctggccg acatcgacgc cctggccgcc aaccgcatgc cgcaccgctg
    41101 gctggcactg accgcgttgc cgccagctgc ggacgaggcg gcgcccagcg ccgggcgctg
    41161 ggccctggtg agcgacagca ccgcgctgcc ctttgccgaa gccagcctgg acctggtggt
    41221 gctgccccac acgctggagc tcagccccga cccgcacgcc accctgcgcg aagtgcagcg
    41281 cgtgctggtg cacgaaggca aggtggcgat tgccggcctc aacccgatca gcctgtgggg
    41341 cctgcgccag tggcgcagcc acctgtaccg cggcctcggc ggcgggcagc cgtttctgcc
    41401 ccaagcgggc gaattcatcg gccactggcg actgcgcgac tggctgcagt tgctgcagtt
    41461 cgagctggaa tcggtcagct tcggctgctg gcgcccggcc gtgcgcagcc cccgccggct
    41521 ggcgcgctac gcctggatgg accgcctggg cgcgcgctgg tggccggtgt tcggcgccgc
    41581 ctatgtcatg ctggcggtca agcgcacgca tggcgcgcgg ctgatcggcg cgcgctggaa
    41641 ggcggcgccc gtggccgcca cggcgccggc gacggtggcc caccgccgcg ccgacgcccc
    41701 caccctgacg gaaaccgaac ttgaaccacg ttgagatcta caccgacggc gcctgcaagg
    41761 gcaaccccgg ccccggcggc tggggcgtgc tgctgcgcgc gcccggccac gaaaaagagc
    41821 tgtacggcgg cgagccgcag accaccaaca accgcatgga actgacggcg gtgatcgagg
    41881 gcctgcgcgc gctcaagcgc ccgtgcgaag tcgatctgta cctggacagc cagtacgtgc
    41941 gccagggcat caccgaatgg atcgccggct ggaaggccag gggctggcgc accgccagca
    42001 agcagccggt caaaaacgtc gatctgtggc agcagctcga tgccctggtc ggcggcgccg
    42061 gccatcggat ccggtggcac tgggtgcgcg gccacaccgg ccacgaaggc aacgaacgcg
    42121 ccgacgcgct ggccaaccgt ggcgtgccgc gcggctgaac ggtgcgtgca ggcgccggtc
    42181 gcagcgccac cgcgcccacc tcggcgcggc cggcgcgcaa ccggtaaatt acgcgcctga
    42241 ttcacacttg ctcacagctt cgatgccagc acctttgcac ccccccatgc cagccgccgc
    42301 cggagcccgc cgtggctagg cgcccgtggg tatcgatggt cgccgtcggg gcgctgctgg
    42361 cgctgtcggc cggcgcctgg tggtggcagc gcgcgggcgg cagcgcgccc gcgggtggcc
    42421 cggtgccggc cgccagtgcc gcgcgcgggc cgggcaacgg cgcggccggc ggggccggtg
    42481 gcggggcgcc gccggtggtc gagctggcaa cggcgcgggc gatggtgctg agcgactcgg
    42541 ccagcgccgt cggcagcctg cgtgcgcgcc agagcgtggt gctgcggccc gaggtcgccg
    42601 gacgcgtgac gcagctcggc tttcgcgacg gcgagcgcgt gcggcgcggt cagttgctgg
    42661 tgcagctcga cgaccggctg gcgctggcgc aggtgcagca ggcgcgggcc gaactgggca
    42721 tcgccgaatc caaccaccgg cgcaacgccg atctggcggg gcagggcttc atcagcgcgc
    42781 gcgcggtgga cgaaagccag gccgcggtgg cggtggcgcg cgccaagctg gagctggccc
    42841 aggccagcgc cgcgcggctg aagatcctgg cgccgttcgg cgcgctggcc ggcatcggca
    42901 acgtgcacgt gggcgacgtg ctgcaggcgg gcgccgacat cgtgcacctg gacgacctgg
    42961 acgccatgct gctggacttt cgcctgcccg agcgctggca ggggcgggtg cggcgcggcc
    43021 agatcagcca cgtgacgctg gacgcgctgc ccggtcacgc cttcgaggcc gtgatccagg
    43081 ccatcgaccc gcagatcgac gcccagggcc gcagcgtggc ggtgcgcgcc tgcatcgaca
    43141 accgccaggc gctgctgcgc cccggcatgt tcgcgcgcgt ggacacccgc tttggcgagc
    43201 gcagcggcgc cgtcatgctg gccgaggagg cggtgctgtc gcagggcggc aacctgtacg
    43261 tgtaccggct ggccgcgggg ccggcggcgg gcgcgtcggc gcccgaaggc accgccgagg
    43321 tcgagcgcgt gcccgtcacc ctgggggcgc gccaggccgg cctggtcgag gcggtaagtg
    43381 gcgtggccgc cggcgaacgc atcgtggtgg ccggccagca gcgcctgaac gcggcgcgca
    43441 cgcgcgtgcg cctgacgcgc gcggcagacg cggccgccgg cgccgacggc ccggcgccgg
    43501 cggccagcgc cgccgcggcg gccccggcgc ccgccgcacc gctgcagtgg caccgcgccg
    43561 ggtccggcgg caacgcctgc gcggcggcgg cgcgcggctg agggggcatg cccatgccga
    43621 cggcgccctt cgatactacg aaaatgatag ctgctggcgc ttgctgcata agcgccatag
    43681 cctgtttttg cttaaaaccc agcgtgccga gcagtgccca tgcaactggc tgaagtctcg
    43741 atccgccgcc cggtgctggc caccgtgctg tcgctgctga tcgtgctgat cggcctggtc
    43801 agctacagcc ggctggcggt gcgcgaatac cccaagatcg acgagccggt ggtcaccgtc
    43861 agcgtgcgct acgtcggcgc ctcggccgag gtgatcgaga cccaggtcac caagccgctg
    43921 gaagactcca tcgccggcat cgacgcggtg gacgtgctga cctccatcag ccgccccgag
    43981 caggcgcaga tcagcgtgcg cttccggctc gaaaaggacg ccgacaccgc cgccgccgag
    44041 gtgcgcgacc gcgtctcgcg cgtgcgcaac cgcctgccgc agagcatcga cgagccggtc
    44101 atcgccaagg tcgaggccga cgccttcccg gtcatctggc tggccttttc cagcgacacc
    44161 cgctcgcgcc tggagatcaa cgacctggtc aaccgcatcg tcaaggcgcg cctgcagacg
    44221 gtgaccggcg tggccgacgt gcgcatcttc ggcgagcgca agtacgccat gcgcgtgtgg
    44281 ctggacccgg agcgcctggc cgcctaccgc ctgaccacgc aggacgtcga agacgccatc
    44341 cgccgcaaca acctggagct gcccgccggc cgcatcgaat ccagccagcg cgagttctcc
    44401 gtcacctcgc agaccgacct ggcgcggccg gcgcagttcg ccgacatcgt gctgcgcacc
    44461 gtcagcggct ttccggtgcg cctgcgcgac gtcgcccggg tcgaggaggg tgccgccgac
    44521 gagcgcagcg ccgtgcgcct gaacggccgc tcggccatct cggccggcgt gatccgccag
    44581 accaccgcca acccgctgct gctgtcgcgc ggcgtgcgcg acatgctgcc gcgcctgcag
    44641 gacgatctgc cgccggacgt caagatcgac atcgccaacg acaactcggt gttcatcgac
    44701 cgcgccgtca gcagcgtcta caccaccatc accgaagcca tcgtgctggt ggcgctggtg
    44761 atcttcgtct ttctgcgcac ggtgcgcgcc tccatcatcc ccatcgtcac catcccggtc
    44821 agcctgatcg gcgccttcgg gctgatgtcg ctggccggct tttccatcaa caccctcacc
    44881 ctgctggcgc tggtgctggc catcggcctg gtggtggacg acgccatcgt catgctggag
    44941 aacatccacc gccacatcga agaaggcatg gcgccgtttg ccgccgccat ccacggcgcg
    45001 cgcgaaatcg gctttgccat cgtcgccatg acgctcacgc tggcggcggt gtacgcgccg
    45061 ctggccttca cccccgggcg caccgggcgg ctgttcgtcg aattcgcgct ggcgctggcc
    45121 ggcgcggtgc tggtgtcggg cttcgtggcg ctcaccctgt cgcccatgat gtgctccaag
    45181 ctgctgcgcc acaacccgcg ccccggcgtg ttcgaccgcg gcatggagcg cgccctcatg
    45241 ggcctggcgc gcgcctacgg tcgcctgctg cggcgcgtgc ttacgcgcgc gcgagcgctg
    45301 gtgctgctgg gcatggccgg ctgcgccgcc gccatcgcct ggctctaccc cagcatgcgg
    45361 caggagctgt cgccgctgga agaccgcggc gtgatcctgg ccaacatcaa cgcacccgac
    45421 ggcgccacgc tcgactacac cgaccgctac gcgcgccggc tggaagccat cggccagcag
    45481 taccccgagt tcgaccgcat cttcgccaac atcggcaacc ccagcgtgtc gcagggcagc
    45541 gtggtgtacc gcgcggtcga ctggagcgag cgccggcgct ccacgctcga catcgcgcgc
    45601 cagatgcagc cgcagttcaa ccagctggcc ggcgttaacg cctttcccat cacgccgcct
    45661 tcgctggggc agggctttcg cgagcgcccg gtcaacttcg tggtgcagac ctccgacagc
    45721 tacgacaacc tcaaccgcgc catgcgcgcg ctgatggacg aggtggccgc caaccccggc
    45781 ttcatcgccc ccgacgtcga cctgcgcctg tccaagcccg agctgcgcat cgaagtcgac
    45841 cgcgaacgcg ccgccgacct gggcgtcagc gtcgacgcgg tggcgcgcgc catcgaaacc
    45901 atgctgggcg ggcgccaggt cacccgctac aaacgcgacg ccgagcagta cgacgtcatc
    45961 gtgcagacca ccgccagcgg ccgcggcacc ccggccgaca tcgaccgcat ccagctgcgc
    46021 gccagcaagg gcggggtgga caccatggtg ccgctgtcca gcctggtgca ggtgcgcgaa
    46081 ggcgtggcgc cgcgcgagct caaccacttc ggccagcgcc gctcggccac gctcaccgcc
    46141 aatctggcgc ccgattactc gctcggccag gcgctgacgt tcctcgacca ggccgcggcg
    46201 cgcgtgctca agagcggcta caccaccgac ctgaacggca tctcgcgtga attccgctcc
    46261 agccagggcg cgctggtggt ggtgttcgtg ctggcgttgc tgttcatctt cctggtgctg
    46321 gcggcgcagt tcgaaagctt cgtcgatccg ctggtcatca tgttctcggt gccgctgtcc
    46381 atgatcggcg cgctgctggc gctcaaatgg agcggcggtt cgctcaacgt gtattcgcag
    46441 atcggcctca tcaccctggt cggcctggtc accaagcacg gcatcctgat cgtcgaattc
    46501 accaaccagc tgcgccagcg cggctgggac atgctcgacg ccctggtgca cgccagcagc
    46561 cagcgcctgc gccccatcct catgaccacc ggcgccatgg tgctgggcgc cgtgccactg
    46621 gcgctggcca gcggtgccgg ctccgaaagc cggcgccaga tcggctgggt catcgtcggc
    46681 ggcatgagcc tgggcaccct gctcaccctg ttcgtcgtgc ccaccgtcta cgccctgctg
    46741 gcgcgccgcg ccgtgcccgg cgccaacacc accccggtcg aagcgccaca ggccgcccag
    46801 ccggcgccca cgggccactg aacaccttgt ttcgcctgat cccgagaggc accccatgac
    46861 ccgcccgagc accccccgcc gcgccctgct ggccgccctg accctgtgtg gcggcgccgc
    46921 cttcgctgcc ggcagccacc atggcccgca cggcgagcac gccgccaccg acagcgccat
    46981 cggccagccc ggcgtggccg cgcgcgccag ccgcagcgtg cgcatcgaca tgaacgaccg
    47041 catgcgcttc agccccgaac gccttgccgt gcgccagggc gaaaccgtgc gcctggtggt
    47101 gcgcaacacc ggccaggtgg cgcacgaact cagcctgggc accctgcagg atttgctggc
    47161 gcaccgcgac cagatggcac gccagcccgg catgacgcac gccgaaccga accagatcac
    47221 gctggccccc ggccagcagg gggaaatcgt ctggcacttc agccgcgccg gcaccgtgca
    47281 cttcgcctgc ctcatccccg gccactacga agccggcatg aggggtgaaa tccagatccg
    47341 cagcagcggt caacactgag cgcaaggacc gccccagtgc gcctggatgg gagggcggcg
    47401 ctttgaagtc tgcgctcgat cacagcccgc gaagcgccgc ggcatccacc tccgtcccct
    47461 cgtcacccct ggttcaacgg gttcgccact ggccgatgtc gcatcgtcgg ttcactcgat
    47521 gttctgcacc tgctcgcgca tctgctcgat cagcaccttc atgtcgacgc tgatgcgcgt
    47581 gagttccagc acggccgcct tcgaacccag cgtgttggct tcgcggtgca actcctggat
    47641 caggaagtcg agccgcttgc cggtttcgcc gccctggtgc agcaccgctt cgatttcgtc
    47701 caggtgtgcc gccagccggc ccagttcctc ggccacgtcg atgcggatgg cgtaggccgt
    47761 ggcctcggcc agggcgcgct cctgcgccgc ctcggcgccg atctgaccct cgccgctggc
    47821 cagcgcttcg cgccagcggt ccagaaagcg ctgcttctgc gcctgcaccg cctgcggcac
    47881 cagcggttcg gcggcgcgtg ccagcgcgcg cagttgggtg acgcgttcgc cgatcaggcg
    47941 cgccagccgt gcgccttcgc gctcgcgtgc ggcaacgaag tcgtccagca cccggcgcgc
    48001 cagcgcccgc agcgcctcgg ccgcggcctc gtcgctcagg ctgtcggtgc cgcccgccag
    48061 gcgcagcacc tcggccaccg acaggcgcgt ggcctgtggc agccagttgc acacctggtc
    48121 ctccagcgtg gccagtcgct gcagcaggcg tggcgacggc tcggccagcg cgtcatcggc
    48181 ctggccctgc agccaggcgc gcaactccac cttgccgcgc cgcaggcgct ggcccagcag
    48241 ctcgcgcagc gccggttcgt gcggacgcag ttcgtcggcc agccgaaagc tcaggtccag
    48301 aaagcggctg ttgacggagc gcaactccat gcctacgccg ccccgtgcat tcttggcgcc
    48361 ggagccgccc tcggcgtcgc cggcgagggc ttgctgaccg ctggcgtaac cggtcatact
    48421 gtaaactgac atattacttt tccgggtaca gggtggtctt ggcgacgggt cccagcggga
    48481 tcggtgcgcg acccagtggc ccgggccgat cgacgccgca aattatgtca aaggccaagc
    48541 ccgcgcccct gccgcccgat accgagatcg gcggctaccg cgtgctgcgc aggatctcgg
    48601 ccggcggctt cggtgtggtg tacctggcgg tcgaccgtga aggccagcag gtggccatca
    48661 aggaatacct gccctcggca ttgacattgc gtgaaccggg cgaactggtg ccccaagtgg
    48721 cgcccgaaaa gctgtccctg taccggctgg gtctcaagag ctttttcgag gaaggccggt
    48781 cgctggcgca gatctcccac ccgtcggtgg tgagcgtgct gaacttcttc cgccagaacg
    48841 agaccgtcta catggtgatg aattacctgg agggcgcgac cctgcaggat ttcatcgtca
    48901 ctgcgcgcga gttgaagaag cagaaggtgt tccgcgagtc caccatccgg tcgctgttcg
    48961 acgagatcct gcgcggtctg cgcatcgttc atcagcacaa gatgctgcac ctggatctga
    49021 agccggccaa catcttcatc accgacgaca acaaggcggt gatgatcgac tttggcgctg
    49081 cgcgcgaggt gctgaacaag gaaggcaact tcactcgtcc catgtacacg ccaggctttg
    49141 ccgcacccga gatgtaccgg cgcgactcca ccatgggacc gtggaccgac gtgtacgcca
    49201 tcggtgcctg catctattcg tgcatgcacg gctatccgcc caacgacgcg ccgcagcgac
    49261 ttgaaaagga ccgtctgtcg ctgtcgctga cgcgcatgcg cggtgtctac tcggacaacc
    49321 tgatcgaggt ggtcgaatgg tgcatgtcgc tcgacccgct gtcgcgcccg caatcggtat
    49381 ttgcgctgca gaaggaactg tcgcgcgaag gtgagcgcca gtacacccgc ctgtcggtcg
    49441 gcgagcgcgt gcgcctgcag ttcgacaaca tgatgaacga caccaagcag agtctgaagc
    49501 gggtcacgcg cttcggtgcg cgtccgaagt aagttcttct gccatgaaat tttcggtctt
    49561 ccagctcagc cgccgcggcg ggcgtgagaa aaacgaggat cgcatgggtt acagctacac
    49621 gcgcgatgcg gggttgtttg cactggccga cggcatgggc ggacaccccg agggtgaagt
    49681 ggcggcccag attgccttgc agacgatgtc ggcgctgttt cagcgcgagg cgcggccgag
    49741 cctcgcggtg ccgaccagct ttctggcgtc cagtctgatg ggcgcgcacc accagatcat
    49801 tcgctatgcc agcgagcgcg gtctgctgga taccccgcgt accaccctgg tggctgccct
    49861 ggtgcaggaa ggggagctgc actggatgca ctgcggcgac tcgcgactgt acgtggtgcg
    49921 cgacggccag ttgctggtgc gcacccgcga ccattcgtac agcgaggcgc aggccggtgc
    49981 cggcatgaca tccgaagcca tcaaccgcaa cgtgctgttc acctgcctgg gctcgaccgt
    50041 acggccgatg ttcgacatcg ctggcccgct gcgcctgatg cagggcgacc gcgtgctgct
    50101 gtgctccgat ggtctgtggg gcagcctgga cgatgacctg atcgtgcgca ccctgggcgc
    50161 gctgccggtg gccaaggccg tgccggcgtt ggttgatgtg gccttgcaga acgcgggcga
    50221 acactcggac aacgtcacgg tgctggcgtt cgagtgggaa agcccgcccg aatcggcgtt
    50281 cgagcacagc ggcttcaccg aaacccaggt gctgagcgac gaggtgttcg cctccaccat
    50341 ccaggccggg gcctacgatc cgctgctgga cgacctggac gacgaggcca tcgagcgctc
    50401 catcgccgag atcaatgccg ccatccagcg cacggccatg aaccgccgct gagcgggtgc
    50461 gggccgcggc gtggcatgct gcgcgcctgg ccgactttct tcgacaccat gaatgcctcc
    50521 gtttcgtccc ccgcgcgcac cgaccgtgcg cccgaccagt tgcgcccggt gcgcatcacc
    50581 cgccactaca cccgctacgc cgaaggttcg gtgctgatcg aatttggtca cacccaggtg
    50641 ctgtgcaccg cctcggtgga agaaaaggtg ccgccgcaca agcgcggcag cggtgagggc
    50701 tgggtcacgg ccgaatacgg catgctgccg cgcgccaccc acacgcgggg cgaccgcgaa
    50761 gcggcgcgcg gcaagcaaag cgggcgcacg caggagatcc agcgtctgat cgggcgcagc
    50821 ctgcgcgccg tgttcgacct caccaagctg ggcgagcgca gcatcctgct ggactgcgac
    50881 gtgttgcagg ccgacggcgg cacgcgcacc gcggccatca ccggcgcgtt cgtggcggcg
    50941 caggacgcgg tgagcggcct gctggcgcgc gggctgatcg ccgagtcgcc gatcctgcac
    51001 ccggtggcgg cgatctcggt aggcatcgtc ggcggcgtgc cgatgctcga tctcgactac
    51061 gcccaggact catcctgcga caccgacatg aacgtggtca tgaccggcgc cggccactac
    51121 gtcgaggtgc agggcacggc cgagggcgcg gcgttttcgc gcgccgagat ggaccagctc
    51181 ctgcggctgg ccgaaaaggg cattggcgag ctgatcgaga tgcaacgccg ttcgctattg
    51241 aattgatagc ttgtggcgct tgctggacaa gcgccagagg gtgatttgaa tgagaatcgt
    51301 gctggcctcc aacaacccgg gcaagctggc cgagctgcaa gccatgctgg ccccgctggg
    51361 catggacctg gtgcgccagg ctgaactggg catcggcgag gcggccgagc ccttccgcac
    51421 cttcgtggaa aacgcgctcg ccaaggcgcg ccacgccgcc gcggccagcg gtctgccggc
    51481 gctggccgac gacgccggcc tgtgcgtcga cgccttcggc ggcctgccgg gcgtggacac
    51541 ggcgtactac ggcacccagt tcggctacgc caaaggcgac gccggcaacg tgtgcgcgtt
    51601 gctggagcag atgcagggca tcgacaatcg ccgcgccgcc atggtcagca ccctggtggc
    51661 gctgcgccgc gccgacgacc ccgagccgct gatcgccgtc ggacgcgtgg tgggcgagat
    51721 cgcgcgcgcg ccgcgcggca gcgggggctt cggcttcgat ccggtgatgt tcatccccga
    51781 gcacggccag acctttgccg agatgccgac cgacacgaag aacgccctca gccaccgtgg
    51841 tcgcgccgtg gcggcgatgg tggccttgat gcgcgagcgc tggctgtgaa caacgacgtc
    51901 cggcactacc tgcgccccgg caccctgcag ctgccggcgc tgccgccgct gtcgctttat
    51961 ctgcacctgc cctggtgcct gaagaagtgc ccgtactgcg acttcaactc gcacgagcgg
    52021 cgcaccggtg tcgacgcgcg ggcccagcag gggcgctacg tcgacgcgct gctggccgac
    52081 ctggaggcca gcctgccgct ggtgtggggg cggccggtct acagcgtgtt catcggcggc
    52141 ggcacgccca gcctgttcga gccggacgac atcgaccgtc tgatcgccgg cgtgcgcgcg
    52201 cgcctgccgc tggagccgat gtgcgagatc acgctggaag ccaaccccgg caccttcgag
    52261 cgcgagcgtt tcgcggcgtt tcgggcggcc ggcgtgacgc gcctgtcggt cggggtgcag
    52321 agctttcatg accggcacct gaaggcgctg ggccgggtgc acgacggcgc ccaggcgcgc
    52381 gccgcgctgc aggaagcggc gcgcgctttc gacaccttca acctcgacct gatgtatgcg
    52441 ctgcccggcc agaccctggc cgagctggag caggacctgc gcacggcgct cgactttgcg
    52501 ccgccgcacc tgtcgatcta tcacctgacc atcgagccca acaccgtgtt cgccaagttc
    52561 ccgccgcgcc tgccggacga ggacacggcc tgggacatgc tcgaccgcat caccgcgctg
    52621 accgccgacg ccggcctggc gcgctacgaa gtgtcggcct acgcgcgccc cggccaccgc
    52681 tgcgtgcaca acctcaacta ctgggaattc ggcgactacc tggggctggg cgccggtgcg
    52741 cacggcaagc tgagctttcc gcaccgcatc gtgcgtcagg tgcggcaccg ccagcccgag
    52801 cgctacatgg agcaggcgct ggccggcgac gccgtggcgc aggcgcacga agtggcgcgt
    52861 gccgagctgc cgctggaatt catgctgaac gcgctgcgcc tgaaggacgg cttcagcctg
    52921 accgactaca ccgagcgcac cggcatgccg cccagcagca tcgccagcgc gctgcagcag
    52981 gccgagcagt ccggctggct gcagcgcgag ggggcctgga tccggccgac ggcgcgcggt
    53041 ttcgactttc tgagcgatct gcagcagctg tttctgcccg aaggctgagc ctggcggcgc
    53101 gccgtcagat ctggctgagc aggtcgggtg ccggaaagcc ggtggcgtgg accagggctc
    53161 caagcagggc ccgccagtcg gtgccgacct cgatcagcgc catctgcgcc gcccccatga
    53221 aaccgctggc cacgctgtgc tgcagaaagc tcagcatggc gtcgtagtag ccgtcctggt
    53281 tgagcaggcc caccggcttg tcgtggtagc cgagctggcg ccaggtccag gtctcgaaga
    53341 actcttcgaa ggtgccgatg ccgcccggca gcgccagaaa ggcgtcggcc cgttcggcca
    53401 tctggcgctt gcgctcgtgc atggtttcca ccacatgcag ctcgtggcag ccgcggtgcg
    53461 cgtgctcgcg ctccaccagc acgcgcggga tgatgccgac cacgcgcccg cccgccgcca
    53521 gcgtggcgtc ggccaccacg cccatcaggc cggcgcggcc gccgccgtac accagctggc
    53581 cgccgtggcg accgatccag tggccgacct gggcggcgac gtcggcaaac gcctgcgcct
    53641 cgcccgggcg cgagccacag tagacacaga tggaaaaagc aggttcagaa gcagcagcag
    53701 cggtcatgag gagaacaggt gggtgtacag cgcggcgagc cagatacccg cccccagcag
    53761 caggctcagc agcacggcgg ccgaacccat gtccttggcg cgtttggaca aagcatgcca
    53821 ttcggggccg atgcggtcga tggccgactc gatgccggag ttgagcagct cgaccatcag
    53881 caccagcagc agcgagccga ccagcagcgc cacctcgacc caggtgcggc caagccacag
    53941 cgccgccggc agcagcacca gggccagcag ggcttcctgg cggaaggcgg gttcgcccca
    54001 gccggcccgg agcccggcca gcgaatggcc tgtggcgtgc cacagccgcg ccagcccggt
    54061 gcgccgctgg tgcgcatggg cgggcgcttg ctcgcgaggg cgatcggtgg gcggcatggc
    54121 gtcggggagc gcggcggcac ggatcactgg cgacgcctgg cggcatcggg ctgcacgtcg
    54181 atcaggcgcg tgccggccca ggcgtcgtgc cagaactgct gttcgcgctg aaaccggctc
    54241 agcagggccc agacggcgat ccagccgaac atcagcacgg tgacctcact gcccgacagg
    54301 cgaaacggcg ccatggccgc cagcggcggc agaaaccaca gccaggccag caggtagcgc
    54361 agcagcgcgc gcggccagcg cagcggcagg ccgtgccggt cgaccacgcg gatgtgccag
    54421 gttttcatgg gcagggtctg gcccttcacc caaaagtaca cgaaatacaa ggccagcacc
    54481 acgaacagca gcgcctgcag cgcgtggcgg ttgtccagcg catggcgcgt gttggtggca
    54541 ataccgaaca ccaggcccgg cagcaccacc acgccgaaca tcagcgtgcc ttcgtacagc
    54601 cagcaggcca tgcggcggcg cagcgagggg gcgcgcagcg gcgagcctgc gacggcaatg
    54661 ggcgtggatg gggtggcggc ggaggagttc atgcaacaag cggggcgcgg gcgcccgggt
    54721 ccgacacgcc ccggagcaaa gccaccggcc gctcagcgcg attcggtgtt gggcgcggcc
    54781 ggcctggggc gctggggcag cagtgtattg cggttgaccc gggccggcac cggcgcactg
    54841 gcgggggctt caggcagcac cacgggcacc ggcgggcgcg ccgggcgcac cagccgttcg
    54901 gccggcacgg gccgcagcgc cggcgccgcg cctgccggag gcttgggccg ctcgcgcgcc
    54961 aggcgggcgc gttcttcttc gggcagcgcc tggtattcct cccatttggc gcgccgttcg
    55021 tcggtgggca ccttgcgcac ctcgccgaaa ttgagccgag cctgggtgcg ctgggccggt
    55081 gtgaggttgg cccattcggt catgcggccc tgcagggttg cgcgctcctg cggcgtcatg
    55141 cgactgaagt tctgggtcag ggcgatccac ttgcgctgct ggtcggaacc cagcgacgcc
    55201 cagtcacgct ccagcggcgc cagcgcggtg cgctgggccg tgctcaggct gcgccactcg
    55261 atctggccac cgacggcggc cgctgagccc ggcttggcca cctcctgggc atgcgccggc
    55321 cacagcgtca acgccgtcag ggcaagcaac agaaccggca ggaaatggcg catcaggggc
    55381 aatgccagcg gcccatgggt ctgaatcact ggctgagggt ggaagaggtg cggccgagat
    55441 cggacttgat gaattggagg aaacccggat cggcgtaagc ctccggcggc agatcgtcgg
    55501 tcagcagggc ggcgtcgagc tcggccacct cggtggcgcg gtcgttgtcc tgcaccacat
    55561 cgatggcgac caggccggcc accagcacca gcatcaaggc ggcggacgcc aggcggcccc
    55621 agaagccggc gccgttgtcg cccgcgccga gcgtcagggt tgcgccctga cgcaccgctg
    55681 cccagctggg gcgcgcgacc cgctgtgtca ccggctggcg gcgctgggcc agcgcgcgct
    55741 cgcgcgccgc ccgcagacgt tcgcgcacgt cgtgcggcag ctcatgggtg ccggccgaca
    55801 ggcgcgcgcc cacgcgcagg ccgaagcggc tgcgcagcga ttccgtgtgg tgttgactga
    55861 aattcgtgtt catggctgga tacccttggc tctcaaggcc ttcgccagcg cctggactgc
    55921 gcgcgagcag tgcgtcttga cactgccttc ggaacacccc atgaccgctg ccgtttcggc
    55981 aacatccatt tcctcccagt aacgcaacag aaatgcctcg cgttgacggt tgggcagggc
    56041 ctggatttgt aactcgattt cgtgcagcac ctgcgcgcgc cgggtggcgt cttcggcgct
    56101 ttcgatctgc gtgctgtcgc tggcgccggc gaagatctcc agcaggtcga attctccgtc
    56161 gccatcttcg gcgtcgaagt cgctcatgtt ggaaaacagc gcgttacggg tcttctggcg
    56221 acgaaaccag tccagcgtgc tgttggacag gatgcgctgg aacagcagcg gcagctcggc
    56281 ggccggcttg tcgccgtagt gctcggccag cttcatcatg ctgtcctgca cgatgtccag
    56341 cgccgcgtcg tcgtcgcgca cgtggtagac cgtgcgcttg aacgcccgct tctcgacgtt
    56401 gcgcagaaag tcggagagtt cctgatcggt ggccaaggaa atgtgcggcg ggcgagacgg
    56461 tgaacgggcg cctcgccgcg aggtgcaagg ggcgggcaag ggcagtgcgc gcgagattat
    56521 ggcaccgggg gcgaacacga tttttgggag tcggcgcagc atgccatgtt gcagtgcgat
    56581 aatgcgtgtt gcagtcatca gagcaaacgg gtcagcgccg gcggcagaag tgaggccgcc
    56641 catggtgtct ggcctcaagt cctgaagcgg cctcacaagg gctggcaagg ggcacccaag
    56701 gtttttcgtc agagaaattc tcgaaaggtt gaacgacatg gaaatcagca aagccgaact
    56761 gaagtcggcc gccgtcgcgg cgtccgccga cacttcccag gccccgcagg agctgcgcgg
    56821 cgccgaaatc ctcatcaagg ccctgcaggc cgaaggcgtc aaatacgtct ggggttaccc
    56881 cggcggcgcc gtgctgcaca tctacgacgc cttctacaag caggacacca tccagcatgt
    56941 gctggtgcgt cacgagcagg cggcggtgca cgcggccgac ggctacgcgc gtgccaccgg
    57001 cgacgtcggc gtggccctgg tcacctccgg ccccggcgtg accaatgccg tcaccggcat
    57061 cgccaccgcc tacatggact cgatcccgat ggtgatcatc accggccagg tgccgacggc
    57121 cgccatcggc caggacgcct tccaggaatg cgacaccgtg ggcatcacgc gccccatcgt
    57181 caagcacaac ttcctggtca aggacgtgcg cgacatggcc gaggtgatga agaaggcctt
    57241 tcacatcgcg cgctccggcc ggcccggccc ggtggtggtg gacatcccca aggacgtgtc
    57301 gttcgagaag acggcctaca agggctatcc gcagagcgtt gagatgcgct cgtacaaccc
    57361 ggtcaggaag ggccacggcg gtcagatccg caaggcgctg caactgctgc tggcggccag
    57421 gcggccctac atctacaccg gcggcggcgt gctgctggcc gacgcctgcg ccgagctgcg
    57481 cacgctggtc gatctgctca attgcccggt gacccacacg ctgatggggc tgggcgcgtt
    57541 cccggcgtcg gaccggcgct ttctgggcat gctgggcatg cacggcacct gggaagccaa
    57601 caacgccatg cagcactgcg acgtgctgct tgccgtgggc gcgcgcttcg acgaccgcgt
    57661 gatcggcaac cccaagcatt tcgcgcagaa cgagcgcaag atcatccaca tcgacatcga
    57721 cccgtcgtcg atctccaagc gcgtcaaggt cgacgtgccc atcgtcggcg acgtcaagga
    57781 cgtgctgacc gagatgatcg gcatgatccg cgaggccggt gcgcgccccg atgaggccgc
    57841 gctggccgac tggtggaaga cggtcgaggg ctggcgcgcg cgcgactgca tgaagtacga
    57901 ccgcgccaac accgacgtca tcaagccgca gaaggtgatc gaaaccctgt gggaaatgac
    57961 ccgcggcgcg gacgcctaca tcacctccga cgtcggccag caccagatgt gggcggcgca
    58021 gtactaccgc ttcgacgagc cgcgccgctg gatcaactcg ggcggcctcg gcaccatggg
    58081 cgtgggcatt ccctacgcca tgggcatcaa gctggcccgg cccgacagcg aggtgtactg
    58141 cgtgaccggc gaaggctcgg tgcagatgtg catccaggag ctgtccacct gcctgcagta
    58201 caacacgccg atcaagatca tctcgctcaa caaccgctac ctgggcatgg tgcgtcagtg
    58261 gcaggaattg cagtacgccg gccgctacag ccacagctac atggacgcgc tgccgaactt
    58321 cgtcaagctg gccgaagcct atggccacgt cggcatgctg atcgagcggc cgcaggacgt
    58381 cgagcccgcg ctgcgcgagg cgcgcaagct caaggaccgc acggtgttca tggacttccg
    58441 caccgatccg accgagaacg tgttcccgat ggtggagccc ggcaagggca tcaccgaaat
    58501 gctgctgggc tcggaagagt tgtaagaaaa acggctctgg cgcagactgg atcagcgcca
    58561 gcagctatcg tattaatagc aagacgaatc tattgtccgc caagcccgcg cattaccgtg
    58621 cgcgggggag ggcggcgaaa agaggaatcg acccatgaaa cacatcattg ccgtactgat
    58681 cgagaacgaa gcgggcgccc tgtcccgtgt ggtgggcctg ttttcggccc ggggctacaa
    58741 catcgagtcg ctcatcgtgg cgcccaccga agaccccagc ctgtcgcgca tgaccatcca
    58801 gacgcgcggc tccgacgagg tgatcgagca gatcaccaag catctcaatc gcctgatcga
    58861 agtggtcaag gtggtcgacc tgaccgaggg cagctacatc gagcgcgagc acatgatggt
    58921 caaggtgcgc gccgtcggca aggagcgcga ggaaatcaag cgcatggccg acatcttccg
    58981 cggccgcatc atcgacgtca ccgacaagag ctacaccatc gagctcaccg gcgaccaggg
    59041 caagcacgac gccttcctga acgcgatcga caaatccgcc atcctggaga ccgtgcgcac
    59101 cggcgccagc ggcatcgggc gtggcgaacg actgctgaag gtttgaggcc acagcgcccg
    59161 cccatcctgc gcaagcagct ctttattcca tagcaagcaa agcatcagaa ggagaaaccc
    59221 gtgaaagttt tctacgacaa agactgtgac ctgagcctga tcaagggcaa gaccgt
//
LOCUS       DAOQTZ010000005        56084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_641_length_56084_cov_13.192293, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000005 DAOQTZ010000000
VERSION     DAOQTZ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 56084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56084
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_56084_cov_13.192293"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1085)
                     /locus_tag="PKN51_01260"
     CDS             complement(138..1085)
                     /locus_tag="PKN51_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT83755.1"
                     /translation="MSQRPVQALALPDDLVVLQRDWLSSNNLLCLGEQAAIVDTGHVK
                     HAAQTVRRVRAVLGTQPLATIAHTHLHSDHCGGTGALQAAWPTATTWVPEPSLDAVQR
                     WDEDALSFDGTGQRCARFGADRALVPGQPVRLGAHDWQVHAAPGHDSLAVLLFEPRHG
                     ILVSGDALWEHGVGVIFPHIDGSAGFEPFNETLDVIERLAPEIVIPGHGAPFGRAGGA
                     IDAALGRARERIATFQAQPAQHALYAAKVLIKYQMLDVDAMTHADFERWLNAAPSLHL
                     LHRQHRPDLDWPSWLAHVLAPMFGKGVLQRDATHVRDGN"
     gene            1284..1718
                     /locus_tag="PKN51_01265"
     CDS             1284..1718
                     /locus_tag="PKN51_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83756.1"
                     /translation="MRHVGLVGRARCVATRDAHGHVGVLLMIETPQRIPHEARSEIQA
                     YFRRKLMEFGELKGRPFHLLLRDRDELSQTQRDPVRSSSSRIAAIVAAANVGVDAVTL
                     AEQVADRREQVRQRLSERRQARQESAYVPLAPVTDVAPLAEV"
     gene            complement(2000..2941)
                     /locus_tag="PKN51_01270"
     CDS             complement(2000..2941)
                     /locus_tag="PKN51_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT83757.1"
                     /translation="MARAALPPLPPRDGVGPSCVVVPGAGWPTVLDFLAERLPALTRQ
                     AWQQRLQGGQVLDAQGRPVAAELACRSGLRLYYYRGWADEPALEGAPEQIVFQDDWLV
                     VADKPHFMPVTPAGRFVQRSLLVRLKRRLGLPGLSPIHRIDRETAGLVVLAVQPHTRA
                     AYQGLFRDRAVHKLYEAVAPDRAELAQPRTHRSRLEPDPQRFFISREQPGAPNSETHV
                     ARAAVLGKHALYHLRPVSGQRHQLRLHLCALGAPILGDAFYPHVLRGPDAPDDRTRPL
                     QLLARELAFDDPYTGQRRQFTSGLELAAAAQRWPLTS"
     gene            complement(2952..3713)
                     /gene="trmB"
                     /locus_tag="PKN51_01275"
     CDS             complement(2952..3713)
                     /gene="trmB"
                     /locus_tag="PKN51_01275"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNT83758.1"
                     /translation="MAHPPDAAGPAAQAGADVTHPRAIRSFVTRAGRTTRGQARALAE
                     LGPRFVLPYDQNVPDLIASCASQTSAGGQKYQKAVLEIGFGMGHATAHIAALMPDTLF
                     IGCEVHEAGVGALLKLIGEQGLDNIRIVPHDAVQVLRHMIAPGTLDGIHIFFPDPWHK
                     TRHHKRRLIQPPFVRELAERLRPGGQLHLATDWQPYAEQMLAVLSAEPLLVNTADGYA
                     PRPDYRPQTKFERRGLRLGHGVWDLVFRRRLAEMA"
     gene            complement(3713..4618)
                     /locus_tag="PKN51_01280"
     CDS             complement(3713..4618)
                     /locus_tag="PKN51_01280"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNT83759.1"
                     /translation="MSSSLHPMLNVAIRAARAAGAIINRAALDVEAVRISQKQVNDFV
                     TEVDHASEDAIIDTLLNAYPGHGIHAEESGTTRGNPQSDHVWIIDPLDGTTNFIHGFP
                     VYCVSIALAVKGRIEQAVIYDPTRNDLFTATRGRGAFLNDRRIRVSKRTRLPECLIST
                     GFPYRKGDDFQGYLRMMADVMQRTAGLRRPGAAALDLAYVAAGHTDGFFETGLKPWDL
                     AAGSLLITEAGGLIGNFSGEAGFLEQGECLAGNPRVFAQLVQLLRPHSRFDGAPQPSG
                     EQRAPLSLRKTDEAAQPATTPPLQP"
     gene            4816..5244
                     /locus_tag="PKN51_01285"
     CDS             4816..5244
                     /locus_tag="PKN51_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT83760.1"
                     /translation="MANPIVRSRLFDDFFNDFAPGFFVKPLHGDPLPTAAQIRIDVKE
                     NNGTYTVEAEMPGVGKDDIHVSIDGSVVTVRAEIKQQDSQSSGERVLRSERYYGAVAR
                     SFQLPQDIDQSAAKARYDNGVLTLTLPKKQVSGSQRLTIE"
     gene            complement(5284..6681)
                     /locus_tag="PKN51_01290"
     CDS             complement(5284..6681)
                     /locus_tag="PKN51_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNT83761.1"
                     /translation="MSSPARRAFLRRLGQLGLAGSAAPWAINLAAIGEAAAFSADGYK
                     ALVCVFLYGGNDNGNTLIPYDSASHADYATARQSLAIARANLAGTVLSGSDLPAGREY
                     ALAPQLAPLKPLFDAKRLALQLNVGPLRVPTTLEQYRARSVPLPPKLFSHNDQQSVWQ
                     SSAAEGAVRGWGGRLGDLALAGNEGSALFTCLSVTGNAVFLSGEHALAYQIGAGGAVA
                     IKGVTQDDGMYGAQACRQALRTLITEPRAQVLESELNRITARSLDSHAVLAGALATSV
                     GQFDAVLPDQVDGRRRTLNQQLKMVARLIAARGQLGPRRQVFLVSLGGFDNHDNLMNR
                     HPALLTELGAGLAGFQQALDALGVGPQVTTFTASDFGRTLVSNGDGSDHGWGSHHFVL
                     GGAVRGGAFYGQAPQAVLNGPEDVGQGRLLPTTAVDQYAATLARWFGVSDTELPLVAP
                     AIGQFGTRDLGFLGA"
     gene            complement(6702..8435)
                     /locus_tag="PKN51_01295"
     CDS             complement(6702..8435)
                     /locus_tag="PKN51_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNT83762.1"
                     /translation="METLAPDTALDHAADAAPPEPATQPASSAPSPGSVAALSGLSAL
                     LASGLAACGGGGEATQADPPAAPISAAEAARFLQQAQFSSSDADIAAVRSQGYARWLD
                     AQMQAPSHTSGWDWLVEQGYQQESLRFNGGYADHMMWNQLIAAPDAVRKRVALAWSEI
                     MVVSGNGIDGYWPSFAMAAYWDLLNQHAFGNFRELLQAITLNPAMGAYLNTRGNKKAN
                     ASGRVPDENYAREVMQLFTIGLYELNPDGTLRGGQPQETYGPADVSQLAQVFTGYDLD
                     AASGAYKTDPAPFRRPMKLNPAQHAGDSVTVLGRSIAAGDGTARLRQALDILFEHPNV
                     GPFIGRQLIQRLVTSAPSPAYVGRVAAAFNDNGAGVRGDLRAVTRAVLLDPEARQDPK
                     LNGPAWGKLREPMIRFVQWARSFGATSSDGKWILWDLSDPSTALGQSPLRSPSVFNFF
                     RPGYVPPGTALAERGQPAPEFQLTNENSVAGYLNYMQGAIAWGAWGSKTSKVWVERYT
                     HEMALVQDAAALVERLNLLLAAGQLSPATVATIRDAIATLKPDSDWGRNQRVWSAIAL
                     VMACPEYLVQK"
     gene            complement(8569..9546)
                     /gene="miaA"
                     /locus_tag="PKN51_01300"
     CDS             complement(8569..9546)
                     /gene="miaA"
                     /locus_tag="PKN51_01300"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNT83763.1"
                     /translation="MATPSATEAAGLRPIGIAGPTASGKSALALALAERLPVEIISVD
                     SALVYRGLDIGSAKPSLAERAAVPHHLIDIRDPSQPYSAAEFVRDATRLMAEIRARGR
                     TPLLVGGTMLYFKALQQGLDEMPPADPTVRAQLEARARQHGWAALHAELARVDPASAA
                     RLPPGDSQRIQRALEVWQLSGRPLSSFHATNSVAAGAHQVNAWPLFSLEPQDRAWLHR
                     RIAERFDAMLSAGLLDEVAALRARGDLSPQLPALRAVGYRQAWQALEQGWPLALLRDR
                     GSAATRQLAKRQLTWLRGMPWRRPLPADAADALPRAVQLILATARGNEK"
     gene            complement(9524..10774)
                     /locus_tag="PKN51_01305"
     CDS             complement(9524..10774)
                     /locus_tag="PKN51_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNT83764.1"
                     /translation="MSSPASVTGPGRAPAAMSASTVVVLLGLLLGLQPVTTDVYLPAL
                     PALQHELAASMSQVQLTFAGLLLAFGCSQLVWGPLSDRFGRRPVLLWGMGAYLLASLA
                     SALAPSMPLLIAARVAQGAAMGAAVMCARAIVRDLYEPLEGARMMSRGLSGLGVIAVT
                     CAPVGGLLTELIHWRAALAALALFGALTLALVALRFHETIPARNPRALAPGTLWQAWR
                     RIARHPTFVTYSLLATASYAALFTFLSTSPFVLIEALGLSRFGYGVLMAAGSLVYIAG
                     TMACRRLLQRWGIRRSVWAAGWVTFTAGALMAGLAWLGWGRPWYGWWAVFVPQALFLL
                     AHGVHQPVSQSGTVAPFPDMAGAASALNGFIMMAVAFPMGLWLGRMMDGTPRALALGF
                     FGWSTVIALTAWFLVPRHGDPERH"
     gene            complement(10893..12692)
                     /gene="mutL"
                     /locus_tag="PKN51_01310"
     CDS             complement(10893..12692)
                     /gene="mutL"
                     /locus_tag="PKN51_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNT83765.1"
                     /translation="MPAPRRPIRELPDELISQIAAGEVIERPASVVRELLDNALDAGA
                     SQITVRLLAGGVRLISVEDDGAGIAADELAIALRRHATSKIASLAELESVATMGFRGE
                     ALAAIASIAELTLLSRTAEQPGAWQLDARSGELRPAARAVGTTVEVRELFFAVPARRK
                     FLKSDATELAHCIEALRRHALARPDVGFALWHEGRLIEQWRSHPGAAGLAQRLADVLG
                     EDFLAGSVAIDHQAGGVRVSGHAGLPDRARARADQQYAWVNGRFVRDKVIAHAARAAY
                     EDVLHGQRQPVYVLHVAVDPARVDVNVHPTKIEVRFRDSREVHQAVRHAIEHALATPR
                     AALLARTADPTPPAPAPELFKPFSAPAPARSAQAAIDFKAPAGHAVAELQALWQAAPD
                     AGAAALPAAPADDDWPLGRAIAQLHGIYILAENRAGLVLVDMHAAHERIVYERLKRQL
                     DGARVASQPLLIPATFAATAQEVATAEGCAEVLARLGLEITAFSPRTLAVRAVPTALA
                     AGDAVALARSVLAELAQHEASTVVERARHELLATMACHGAVRAHRRLTHEEMNALLRQ
                     MEATERSDQCNHGRPTWRQLSLRELDALFLRGR"
     gene            complement(12806..14788)
                     /locus_tag="PKN51_01315"
     CDS             complement(12806..14788)
                     /locus_tag="PKN51_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT83766.1"
                     /translation="MSMRKELWFGFSIMALVVASAAWLLLGVEHITNGHLGLLMLSLV
                     VVAIMLGFPTAFTLMGMGMIFTWLAYDRNVTRTLDLMVQAAYKSMANDVLIAIPLFVF
                     MGYLVERANLIEKLFKSLHLAMARVPGSLAVATLVTCAIFATATGIVGAVVTLMGLLA
                     LPAMLRAGYSIPMSAGAITAGGCLGILIPPSVLLILYGATAGVSVVQLYAGAFFPGVM
                     LAGLYVAYVIIVAKLKPAWAPPLSAEERQVDLPTLTSALPAGTRDYAPLRLLNALKGR
                     RNADVPLAHVLRQLAVVVLPGLLFLLLATSTWQAVTKVPAEQAVELQAIGSEGSGAES
                     SDSGLAEPPAEDELQEPPTEDALQEPPGADAPDTLATPPGAEVDAASEPTAAGVAEPP
                     GASAAASDAAGAAQAGTQPAPTWWWVVMGVLGACTALFYLFLSFARLEIFKMLLGSFF
                     PLMILILAVLGSIVFGLATPTEAAAMGSLGGFLLAAAYKRLTMDVLRESVYLTAKTSA
                     MVCWLFVGSAIFSAAFALLGGQELIEQWVLSMNLSKLEFMVLAQVVIFLLGWPLEWTE
                     IIVIFMPIFIPLLNHFGVDPLFFGLLVALNLQTAFLSPPVAMSAFYLKGVAPPYVTLN
                     QIFLGMMPFMAIQVLAIVLLYVFPSIGLWLPNLLYK"
     gene            complement(14785..15444)
                     /locus_tag="PKN51_01320"
     CDS             complement(14785..15444)
                     /locus_tag="PKN51_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNT83767.1"
                     /translation="MQKLLLGIDAVSTFIGKAAAWACVLLTALICWEVFSRYALNKPH
                     AWVLDAQIMLYGTMFMMAGAYTLAKNGHVRGDVLYGFFSPRVQAGLDLLLYFLFFLPG
                     VLAMTWAGWGYAQESLAIREQTFSAEPLPLYPFKFVIPLAGAALLLQGLAEIARCVIC
                     LRQGAWPSREADVEEVDVEKLKEMVHVTDADRAAIAVQPPRPSPPSPGHADHGHTDRG
                     A"
     gene            complement(15562..16686)
                     /locus_tag="PKN51_01325"
     CDS             complement(15562..16686)
                     /locus_tag="PKN51_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT83768.1"
                     /translation="MSKPTQTTGRRNLLKGAAVAAGAMSVPMISVAQTTTLRFQSTWP
                     AKDIFHEFAQDYAKKINDMAGGRLKIEVLPAGAVVPAFQLLDAVAKGTLDGGHGVVAY
                     WYGKNQAVALWGSGPAFGMDANMVLAWHNYGGGKELLDEIYKSLNLDVVSKLYGPMPT
                     QPLGWFKKPVAKVEDLKGLKFRTVGLAVDVFTEMGVAVNPLPGGEIVPALDRGLLDAA
                     EFNNASSDRVLGFPDVAKNCMLQSFHQSSEQFEVLFNKTKYDALPAELKAIIDYATQA
                     ASADMSWKAADRYAKDYAEMSAKQGVKFYKTPDAILRAQLAAWDKVTAKKAAENPMFK
                     KVMDSMRTFAQRSGRWQNDTTIDYRMAWNHYFGAKKGAMG"
     gene            16867..17514
                     /locus_tag="PKN51_01330"
     CDS             16867..17514
                     /locus_tag="PKN51_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNT83769.1"
                     /translation="MDVLMMLVDFVLHVDRHLGEFVAAYGAWVYALLFLIVFVETGLV
                     VMPFLPGDSLLFVVGALAGAGHLSWPLACAVLLAAAILGDQCNYSLGRYFGPRVFKWE
                     SSRLFNRQAFDRAHRFYERYGGVTVILARFMPFIRTFVPFVAGVAEMTRHRFTLFNVA
                     GALIWVLGVVSAGYLFGNLPWVQANLSKIIWALILVPGLIALYGGWKAGRAAPSA"
     gene            complement(17695..19089)
                     /locus_tag="PKN51_01335"
     CDS             complement(17695..19089)
                     /locus_tag="PKN51_01335"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT83770.1"
                     /translation="MTPVTPESPGWPPAPGRRRALLQGGSLVLWLGAHQLARGAGILA
                     VRVWPAEDYSRVTVESDTALKASYRLVGTPPRLAVDIDGLTLDPTLRELVAKVRADDP
                     NIAGIRVGQHQPGVVRLVFDLKHEIKPQVFTLGPVAAYQHRLVFDLYPAHPPDPLAQL
                     IAERMRELGRSPAPVPVEPAAAPAHGGTDTLGELIARQIERANELTRRDAARRPAPPP
                     AGAGATERLIIVALDPGHGGEDPGAIGPGGTQEKDVVLQVALKLRERINASSVGGNPM
                     RAFLTREGDYFVPLQTRVEKARRVQADLFVSIHADAFMRPEASGASVYALSERGASST
                     AARWLAQKENASDRIGGVSLGAKDQSVQHVLLDMSTTAQIRDSLRLGDTVLREIGGVG
                     RLHKRSVERANFAVLRNPDVPSVLVETAFISNPDEERRLRSQAYQNELADALISGIRK
                     YFTANPPLARHRQV"
     gene            complement(19086..19592)
                     /gene="tsaE"
                     /locus_tag="PKN51_01340"
     CDS             complement(19086..19592)
                     /gene="tsaE"
                     /locus_tag="PKN51_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNT83771.1"
                     /translation="MSSHAQIVGSPDAPALSLLWRSEDDTAAFARRLAAAPELRQACI
                     ELHGELGAGKTSFVRHLLRALGVAGRIKSPTYAVVEPHQTPDGLAVSHFDFYRFTDPR
                     EWEDAGLRDLFAAPGLKLSEWPDKAAGLLPAPDLALRLTVRDDGARAVRVSAHTPRGR
                     RLLTELGA"
     gene            19629..20678
                     /gene="queG"
                     /locus_tag="PKN51_01345"
     CDS             19629..20678
                     /gene="queG"
                     /locus_tag="PKN51_01345"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNT83772.1"
                     /translation="MRGWARELGFSHLGVADIDLSAAEPGLLAWLHNGFHGRMDYMAA
                     HGLRRARPAELVPGTVSVITARMDYLPRATPEDWQDVELARLGRPQEGVVSLYARGRD
                     YHKVLRGRLQKLQDRIALAIGPFGHRVFTDSAPVLEVELASRSGQGWRGKHTLLLSRE
                     GGSMFFLGEIYVDLHLPRTAPTGAHCGQCSACIDACPTGAIVAPYRLDARRCISYLTI
                     EHDGAIDEALRPLIANRIYGCDDCQLVCPWNKYAGRSALPDFDPREGLGGAQLTDLLD
                     WSEDEFLRRTEGSPIRRIGHERWLRNVAVALGNALAALPPGDARRAPLHAALRRRANH
                     PSALVREHVRWALGR"
     gene            complement(20854..21771)
                     /locus_tag="PKN51_01350"
     CDS             complement(20854..21771)
                     /locus_tag="PKN51_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNT83773.1"
                     /translation="MHHVTLLLPDFSLIALGWLLCRHTALGRGVWQAVEQLVYFFLFP
                     VYLFQSIVRAPLDVQAASSLIGAGLALGAAGIALAWALPHLPLLGRHIDAHDHAASAQ
                     IAFRFNSFIGLAVAERLAGAQGQQLMAVLIGVSVPLYNVAAVWPMARRSGRAIGGELL
                     RNPLILATVLGLVANLLGLGLPDWAATTLSRIGSASIALGLMAAGAGMQLQSLARAKL
                     LGTALLTIRHLLQPLIAWLLARAFGLGAAEAAALLVFAALPTSSSGYVLAARMGYNGP
                     YVAGLVTLSTLLALLSLPWAFTLLERVAP"
     gene            21879..22844
                     /locus_tag="PKN51_01355"
     CDS             21879..22844
                     /locus_tag="PKN51_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugE"
                     /protein_id="HNT83774.1"
                     /translation="MQRRQWIVLAAATLSAGAVCAQAYPSKPIRLVVPFAPGGTTDII
                     ARTIADPLGKALGQAVVVENKAGGGGVIGATEVVRAAPDGYTLSVATVSTTAANPAIN
                     KKIPYDPINDFVPVINIAATPNVIAVHPSFPAKDYASFVALLKKAPGKYSYASSGTGG
                     IGHLQTELWKSLAGVFVTHIPYRGAGPALNDTVAGQVPIIFDNLPSALPFIKGGKLVP
                     LVVAAPERLADLPNVPTFKEIGLPAVNRLAYYGILAPKGTSKEIVDKLNAATRKVLED
                     PAVKKRIADTGSIMVANTPEQFAQQIKDEFAVYKEVVAKQKLTLD"
     gene            23024..23935
                     /gene="xerD"
                     /locus_tag="PKN51_01360"
     CDS             23024..23935
                     /gene="xerD"
                     /locus_tag="PKN51_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNT83775.1"
                     /translation="MAAAAPDDPLIDRFIDALWLEDGLSANSLAAYRRDLTLYAAWLQ
                     ATHRVALAQTQEPHLNAYMAARADGKATSANRRLTVFKRFFRWALREHLVSADPTLRL
                     LAARQPLRVPKTLGEAQVDALLTAPNTGTPLGLRDRAMLELMYASGLRVSELVALRSF
                     HVGFNEGVLRVLGKGSKERLVPFGEQARDWLERYLREARPALLGGRQSDALFVTSAGR
                     TPGTAMSRVMFWNLVKRYARAAGVTAPLSPHTLRHAFATHLLNHGADLRAVQLLLGHA
                     DISTTTIYTHIARQRLKELHAQHHPRG"
     gene            23952..24815
                     /locus_tag="PKN51_01365"
     CDS             23952..24815
                     /locus_tag="PKN51_01365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT83776.1"
                     /translation="MVRCINLHNPLLTKGERIVTVSESSGCAAGLRVAVVTPYFKESP
                     ATLQRCLTSVRAQTYPQVVHYMVADGFPQNALLTQWPGVRHIQLPNGHANFGCTPRGV
                     GAQCALADGFDVVCFLDADNLIEPEHVATVVETVAKAQAAGVALDAVFSLRYLFLPGH
                     EHLRLVAPREDVGSGFVDTSCISLTRSAGFLWGAWCQIPSSLTPICDRVMCWLMQHHR
                     LKVAWTGQRTVLYESSWAHTYVQAGVPVPASGTHDHTLRAVGQGLTPEELWARLRVRW
                     TFVRPIDGAAS"
     gene            24812..25816
                     /locus_tag="PKN51_01370"
     CDS             24812..25816
                     /locus_tag="PKN51_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT83777.1"
                     /translation="MTRAVVTGGAGFIGSHLCEALLIQGVQVTCVDDLSSGSSDNVAH
                     LLAVPGFAFVPGDVVDPALWQRLPAAQRVFHLASPASPVDYQAAPVQTIRTNVLGTLN
                     ALDFARAHGARMLLTSTSEVYGDPQVHPQTEAYRGHVNPVGPRSCYDEGKRCAEALTV
                     AHARQHGTQVRLARLFNTYGPRLRPQDGRVVSTFITSALRAEPLPLHGDGRQTRSFCY
                     VSDTVRGLLALMAQERVTGPINLGNPQEVSMHALAAMVLRLTGSNAGVRTLPAREEDP
                     RCRQPDISQAQRWLEWQPEVPLVQGLAQTIEWFRAQGAVGANRLPCGSQPVVDLAANT
                     "
     gene            complement(25923..27362)
                     /gene="argH"
                     /locus_tag="PKN51_01375"
     CDS             complement(25923..27362)
                     /gene="argH"
                     /locus_tag="PKN51_01375"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNT83778.1"
                     /translation="MSANQLDKKSQAWSALFSEPMSELVQRYTASVDFDRRLASADIT
                     GSLAHADMLAAQGIISAQDLADIRRGMAQIQGEIERGEFSWQLALEDVHLNIEARLTQ
                     LVGDAGKRLHTGRSRNDQVATDIRLWLRGEIDLITGLLRELQGALVEVAQRHIDVILP
                     GFTHLQVAQPVSFAHHLLAYVEMFSRDEQRMLDARKRVNQLPLGSAALAGTTYPLDRE
                     RVARTLGMDGVCQNSLDAVSDRDFAIEFSAAASLVMVHISRLSEELILWMSQNFAFIR
                     IADRFTTGSSIMPQKKNPDVPELARGKTGRVVGHLMGLITLMKGQPLAYNKDNQEDKE
                     PLFDTVDTLRDTLRIFVEMIGGQPNPVSGAKEGGISVNPAAMEAAAQKGYATATDLAD
                     YLVKKGLPFRDAHETVAHAVKAAQSHACDLSELPLKVLQEFHPAIDKDVYQVLSLRGS
                     LNARATLGGTAPAQVQTQIARHRARLALG"
     gene            27473..28525
                     /locus_tag="PKN51_01380"
     CDS             27473..28525
                     /locus_tag="PKN51_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNT83779.1"
                     /translation="MQDSQILSTFGERKRPPKARPAPPPLVFDACQVGVILRAVLYVE
                     AVIGVGLMYLTADLSDWLARVALVTGGALPATLLWLVLACALKRLLARLSWPLQAAAG
                     VALGALAGLYACGTLALLGFAAAPPWLASASTGALLAAGLMAGLLWRVRGRQPAATTA
                     RLAELQSRIRPHFLFNTLNTAIALVRAEPAKAERVLEDLSDLFRHALAEQGDSATVGQ
                     EIELAQRYLEIEQLRFGERLRVEWALDERVHGARLPPLILQPLVENAVKHGVEPSPGG
                     AELRISTQRRGQRALIKVTNTVPAGQGRAGFGIAQANVRDRLRLLHDLEGDFRTALVD
                     GVYQARIEVPLPGRGA"
     gene            28525..29271
                     /locus_tag="PKN51_01385"
     CDS             28525..29271
                     /locus_tag="PKN51_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNT83780.1"
                     /translation="MALQVLVVDDEPLARARLRALLADCREPEAVAAAEAGTAAQALA
                     LAQHQRFDVALLDIHMPGADGMQLACALRALPEPPRVVFVTAHAEHAVRAFEIDAVDY
                     LTKPVRLERLQAALQKTQRVAQLQSAPEPDSAPDWLLITERHRTLRVPLAEVLYLKAE
                     LKYITVRTADASHLLDGSLNQLEERHGGRFLRIHRSTLVARNAIRALVRHQDPDEGEG
                     WAVQLAGLDEALPVSRRQLAAVRAALGEPR"
     gene            29367..30272
                     /gene="hemC"
                     /locus_tag="PKN51_01390"
     CDS             29367..30272
                     /gene="hemC"
                     /locus_tag="PKN51_01390"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNT83781.1"
                     /translation="MSLSITIATRESRLALWQAEHVRALLTQRGHSVRLLGMTTQGDQ
                     ILDRTLSKVGGKGLFVKELETALEDGRADIAVHSLKDVPMELPAGLQLACVMAREDPR
                     DAWVSPAHASLDELPAGAVVGTSSLRRTVLLRALRPDLRIEPLRGNLDTRLRKLDEGR
                     YAGIVLAAAGLKRLGLAARIRHIFDSDAMLPAAGQGALGIEIREGRGELTQALAPLAD
                     QPTWLAVAAERAVSRAMGGSCSMPLAAHATRQGERLHLRAAWGDPEGAPVLVRAEGEA
                     QVSTLAQAEALGERVAAGLRAGGAR"
     gene            30281..31072
                     /locus_tag="PKN51_01395"
     CDS             30281..31072
                     /locus_tag="PKN51_01395"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNT83782.1"
                     /translation="MAAPRVLVTRPAHDAAHWVGQLRQCGIDAQALPLIDIVPEPLAG
                     RLLQARQHLGDYAVAMFVSGNAVTGFMGSNQAAVQLQQALTAIETRAWSPGPGTTAAL
                     QRAGWPAARIDQPAPEAPQFDSESLWAQVAPQVRPGLRVLIVRGGDAAGSVAGRDWLA
                     AQLTAAGAEVDQVLAYRRTAPRLDAAGLARATAAARDGTLWLFSSSEAIAHLRQCLPQ
                     IGWQAARALVTHPRIGAAARAAGFGTVHHSQPTLDAVSASIKSLA"
     gene            31069..32193
                     /locus_tag="PKN51_01400"
     CDS             31069..32193
                     /locus_tag="PKN51_01400"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNT83783.1"
                     /translation="MTSEPSPAAPAAPPPVPAPVATEAGTAAPASPLAPAHAAAPRGA
                     GVSPGLWLAWLLAAAGLVVGLLLWQKLTGMQEQLARQSREAGTQSVEARTLARQAEEG
                     VRDMAGKVAALDARLAELAAYRAQLDELVHSVARARDENLAVDLEAALRLAQDQTQLT
                     GSVEPLLAALRTAERRLARSSDPRLAPVGRAVARDLERVKSASIPDAAGLLARLDQLL
                     RQVDDLPAANEVPHARSAPATTAPAAAAESAWGRLWQSVRDEAQGLLRVSRIERPEAS
                     MLSPEQVFFVRENLKLRLQGARLALLARQYEAARADLTAAAMSLGTWFDPASRRTQAA
                     ATLLQQVQASTRSAELPRVDDSLVALGQAVATAHTVPGTR"
     gene            32199..33479
                     /locus_tag="PKN51_01405"
     CDS             32199..33479
                     /locus_tag="PKN51_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNT83784.1"
                     /translation="MRAALWLMGLFAAAVALALFAGDNEGTVTVFWPPHRVDVSVNLA
                     LLLLLALFVLLYLALRALSAMVQLPRQARRWRTQQRERAMHATLIDAAAQLAAGRFLR
                     ARKAAESALTRVQALSDAAEAPPHAPSLRTLAHMTVAESAHALRDEAMRDQHLEHALD
                     VAGRAGAAPELREGVLLRAARWSLDQRDAGAALARLNELPAGATRRTAALRIKLKAAR
                     QSGHHAEALETARLLAKHGAFSPAAARSLLRGLAASLIDEVHDPAQLQAVWGSLDAGE
                     RAMPELALRAAQRMLALDGPRALVRQWLRPVWEQALAQPGSFGPALRARLVQVLEQAL
                     IATGEASDQQWLALVESAQQAHPRDPSLQYLAGMACRHRGLWGRAQLLLGQAAQSLPD
                     AELRRHAWRALAELAEQRGDEPAAAQAWKQAARS"
     gene            complement(33672..34694)
                     /locus_tag="PKN51_01410"
     CDS             complement(33672..34694)
                     /locus_tag="PKN51_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT83785.1"
                     /translation="MHAPLPDPRLRTATLASHGLIAASLLLVMWQGLLPGLLCVCVGF
                     FCTRWLSPRLALPARQADRQAPRLAATVVALLPLLLLALALPRTRGLILDAPAQYREL
                     LGFLARTVLELRDRLPPDIGAQLPEGAVEIQRAMASYLASKAGTLATTGRAWLSGLLF
                     AYVGLLIGALAAARPSTPGLKPLAAQLHLRVRRFGETFRQIVVAQFWIALFNTCLTAV
                     FLLAILPLTGHRLPYTMALILLTFVAGLIPIVGNLICNGVLTLVGLSVSPGVALACLA
                     FLIAIHKAEYFINAKVIGHRTHMGVWELLSVMFVMEAVFGPAGLVAAPLFYAYGKKEL
                     QASGWV"
     gene            complement(34731..35777)
                     /gene="purM"
                     /locus_tag="PKN51_01415"
     CDS             complement(34731..35777)
                     /gene="purM"
                     /locus_tag="PKN51_01415"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNT83786.1"
                     /translation="MSPSSTPSPLSYKDAGVDIDAGDALVERIKPLARRTLREGVLSG
                     LGGFGALFEVPRRYREPVLVSGTDGVGTKLKLAFEWAMHDGVGIDLVAMSVNDVLVQG
                     AEPLFFLDYFACGRLDVDTAATVVGGIARGCELAGCALIGGETAEMPGMYPPGEYDLA
                     GFCVGAVEKSALLTGRDVAAGDVVLGLASSGVHSNGFSLVRKCIERAAGQLPATLDGQ
                     PFRQAVMAPTRIYVKPVLAALAAPGGIKALAHITGGGLLENIPRVLPAGLAAHLRAGS
                     WPRSELFTWLQATAGIDDHEMNRTFNNGIGMVVVVAPAQAAACAQALRAAGEQVFEIG
                     HIAARGDGAAVQVA"
     gene            35943..36629
                     /gene="hda"
                     /locus_tag="PKN51_01420"
     CDS             35943..36629
                     /gene="hda"
                     /locus_tag="PKN51_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HNT83787.1"
                     /translation="MRQIALDIGLAPEPTLSGFLAGANAAAVQHLRLWTGGATRSPVP
                     TYLWGPPGSGKTHLLRAARQALAEQGARVGWLDPETPPRLPFDEAWDAVLMDDVQRYG
                     AELQHTAFNWFVNAITPPSGRPRAVLAAGGVPPADLPLREDLRSRLGWGHVFELHPLP
                     EPELRAALRQAADARGVFLSDEVMDFVLRRFARDLGSLMQLLDQLDRYALQAQRAITI
                     PLVKAMLENE"
     gene            36669..37346
                     /locus_tag="PKN51_01425"
     CDS             36669..37346
                     /locus_tag="PKN51_01425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT83788.1"
                     /translation="MLKLALFDLDHTLLPIDSDYAWGEFTTRIGWTDPVDFQRRNDEF
                     YARYQAGTLDVREYVRFATEAVRRQGPEQAARAHARFMREVVGPALRPQARALIEAHR
                     QAGHGLLMVTATNEFVTRPIAQAFGIEELIAIELVRDGAGWITGEIAGTPSFREGKVA
                     RVADWLRARGHDWSDVDITFYSDSRNDLPLLEKAQQPVATNPDDALRAIASERGWRVL
                     ELFKEDT"
     gene            37343..38893
                     /gene="pcnB"
                     /locus_tag="PKN51_01430"
     CDS             37343..38893
                     /gene="pcnB"
                     /locus_tag="PKN51_01430"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNT83789.1"
                     /translation="MIKQLIHKLIDSATGGNARKRRGKRVEIGPEGHGINPALVDERA
                     AGVVRTLKEAGYEAYIVGGAVRDLLLGLKPKDFDVATNATPEQVKQLFRRAFIIGRRF
                     RIVHVVFGRGREHEVIEVSTFRAYVDNATAEAIRGNERTAKGQLAGMKHAVDASGRVL
                     RDNVWGSQDEDAARRDFTVNAMYYDPQARVVVDYHGGIADAHKKLLRMIGDPATRYRE
                     DPVRAIRAVRFGAKLAALGFRIEPKTARPLVEVLPLLAEVPQSRLFDEMLKLLQTGHA
                     LASVEQLRKLGLARGIYPLLDVVVERADQPFVKAALQDTDRRVGEGKPVAPSFLLACV
                     LWQDVLAGWNARLARQEHPFPALQDAIDEVFDARVGDVSGRGKLGADMREIWMMQPRF
                     EKRSGQTPFSLVEQPRFRAGFDFMRLRADVGEVSEELADWWQQFSLADDGERESMMTE
                     LRAEQQRSKRQAPRVHRVPAGTLETAPPAPAPDAAATPAKKRRRRRRKPAGAGGSAPE
                     AGPPPAQP"
     gene            38890..39384
                     /gene="folK"
                     /locus_tag="PKN51_01435"
     CDS             38890..39384
                     /gene="folK"
                     /locus_tag="PKN51_01435"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNT83790.1"
                     /translation="MTQPLSHLRAPVLAYVALGANLGDAAASVRAAIEALARLPDTQL
                     QARSSLYRSAPVDAGGPDYVNAVVALRTGLSAPELLAGLQRLEQAAGRQRPYRHAPRT
                     LDLDLLRYGDGRIDSAALCVPHPRMLQRAFVLRPLAEIAPQLVTPAALAAVAGQGIER
                     LEGA"
     gene            39661..40308
                     /locus_tag="PKN51_01440"
     CDS             39661..40308
                     /locus_tag="PKN51_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HNT83791.1"
                     /translation="MLLSKLLPKEGNFFELFNQHADRIVEAANAFSLLVAHYGDLGQR
                     EKYARDVDNAERAADRVTEEVNRLIHTTFITPIDREQIHGLINLMDDVADLLQDSAET
                     MALYDIHHMTEEIVRLTELSVKCCQRVQAAVRLLGKIAEPTTAEATLKTCEEIDRLES
                     DADRVMRSAMSKLFREEPDVREVIKLKAIYELLETITDKCEDVANQIEGIVLENS"
     gene            40324..41334
                     /locus_tag="PKN51_01445"
     CDS             40324..41334
                     /locus_tag="PKN51_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNT83792.1"
                     /translation="METVQAALWVVVLLVALAIVFDFMNGFHDAANSIATVVSTGVLK
                     PTQAVVFAAFFNFIAIFIFHLSVAATVGKGIVQPGVVDTHVVFGALVGAISWNVLTWY
                     YGIPSSSSHALIGGIVGATVAKAGTGPLVMAGVLKTVAFIFVSPLLGYLLGSAMMVAV
                     AWVFRRVRPGRVDKWFRSLQLLSAGAYSLGHGGNDAQKTIGIIWMLLIATGYAGAGES
                     SPPLWSIVSCYAAIAAGTMFGGWRIVKTMGQKITKLKPVGGFCAESGGALTLFIATGL
                     GIPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWVLTIPASALVAALAYWLSLL
                     LF"
     gene            complement(41406..41852)
                     /locus_tag="PKN51_01450"
     CDS             complement(41406..41852)
                     /locus_tag="PKN51_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83793.1"
                     /translation="MKNKTLATWLAFLGGPLGLHRFYLRGFGDLLGWLLPIPTALGLY
                     GIERVQQFGQDDPLSWLLIPLLGFTISACALTAIVYGLMSAEHWNARFNPQADPQAAP
                     GQTHWGTVFGISVALLIGAGVLMASLAFSFQRYFEYQIEEARKISQ"
     gene            42106..42366
                     /gene="rpsP"
                     /locus_tag="PKN51_01455"
     CDS             42106..42366
                     /gene="rpsP"
                     /locus_tag="PKN51_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNT83794.1"
                     /translation="MVVIRLSRGGAKARPFYSIVVADKRARRDGSFIERIGFYNPGAA
                     QGEEGLRIAQDRLTHWLGVGAQPSLTVQRLIKQAAKAAVAAA"
     gene            42381..42947
                     /gene="rimM"
                     /locus_tag="PKN51_01460"
     CDS             42381..42947
                     /gene="rimM"
                     /locus_tag="PKN51_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574720.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNT83795.1"
                     /translation="MVPGLEPAELPADAVEVGRILDAWGIQGWFKVQPYSASPEALFS
                     CKRWWLLPPERAPKPAWPGAVALNIRQARAHTDAVVASAADITDRDAALGLKGARIFI
                     PRSAFPAPAEGEYYWVDLIGLTVVNREGLVLGQVSDLLATGPQQVLVVGHQEAGKARE
                     RLIPFVDAYVDEVDLPARQIRVDWQPDY"
     gene            complement(42979..43257)
                     /locus_tag="PKN51_01465"
     CDS             complement(42979..43257)
                     /locus_tag="PKN51_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNT83796.1"
                     /translation="MTSRVVPAILLLLLVVIHAQLWIGRGSLPNVSALQRKLDQQRSA
                     NQQARLVNEQLASEVEDLRAGLGMVEERARIELGMVKPNEIFVQVAPR"
     gene            complement(43380..44666)
                     /gene="eno"
                     /locus_tag="PKN51_01470"
     CDS             complement(43380..44666)
                     /gene="eno"
                     /locus_tag="PKN51_01470"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNT83797.1"
                     /translation="MSAIVDIVGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDKKRYLGKGVLKAVEHINTEISEAVLGLDAAEQAFLDKTLIDLDGTDN
                     KSRLGANALLAVSMAVARAAAEESGLPLYRYFGGMNGCQLPVPMMNVINGGAHANNNL
                     DLQEFMIIPVGAPSFREALRWGAEVFHALKKIIHDKGMSVAVGDEGGFAPNVENHEAA
                     IQLILQAIQDAGYTAGQDIVLGLDCASSEFFKDGQYVLDGEGGLKLSAAQWTDMLATW
                     VDKYPIISIEDGMAEGDWDGWKLLTERLGDKVQLVGDDLFVTNTKILKEGIDKGIANS
                     ILIKINQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGS
                     LSRSDRMAKYNQLLRIEEDLGDMARYPGRAAFYNLK"
     gene            complement(44750..45040)
                     /locus_tag="PKN51_01475"
     CDS             complement(44750..45040)
                     /locus_tag="PKN51_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNT83798.1"
                     /translation="MPAGYIIALVTITNPQQYEEYKRWSSEAMRVHGAEICVRGDRVE
                     VLEGDWHPGRTVILKFPSFEAAKAFHDSPEYSRARAARQGAAVMRLLCVEGV"
     gene            complement(45044..45901)
                     /gene="kdsA"
                     /locus_tag="PKN51_01480"
     CDS             complement(45044..45901)
                     /gene="kdsA"
                     /locus_tag="PKN51_01480"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNT83799.1"
                     /translation="MKLCGFDVGLRRPFFLIAGPCVVESEQLQMDVAGQLKEITASLG
                     IPFIFKSSYDKANRSSGASFRGPGMDAGLQILARVRREIGVPVLTDVHTEAEVPTVAA
                     VVDVLQTPAFLCRQTDFIRAVAQSGKPVNIKKGQFLAPHDMVNVIDKARAAAAEKGLD
                     TDRFMACERGASFGYNNLVSDMRSLAIMRETGAPVVFDATHSVQLPGGLGQASGGQRE
                     MVPVLARAAVAVGVAGLFMETHPDPARALSDGPNAVPLRHMKALLETLVALDQVTKRQ
                     GFLEDDFGA"
     gene            complement(45898..47571)
                     /locus_tag="PKN51_01485"
     CDS             complement(45898..47571)
                     /locus_tag="PKN51_01485"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNT83800.1"
                     /translation="MTKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYI
                     NVDPGTMSPIQHGEVFVTDDGAETDLDLGHYERFITTRMKKANNFTTGQIYQSVLEKE
                     RRGDYLGKTVQVIPHITGEIQDFIRRGAGVGTPDAVDVAIVEIGGTVGDIESLPFLEA
                     VRQLSLKLGPNHSAFVHLTYLPWIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADR
                     TIPQDEREKISLFTNVPAWGVISMPDVDTIYKVPRLLHQQGLDGLICDKLRLNTPPAN
                     LKRWDALVHEVESPRHEVSIAMVGKYVDLSDSYKSVNEALRHAGMHNHARVHIEHIDS
                     ETITPATAAQLARFDAILVPGGFGERGVEGKIATVQYAREHKLPYLGICLGMQVATIE
                     YARHVAGLEGANSTEFNPATRHPVIALVTEWKDKDGRIQRRDAGSDLGGTMRLGAQSA
                     DVVPGTLAHRIYGDVVTERHRHRYEANVDYLERLRAAGLVISALTQREQLTEIVELPQ
                     GVHPWFVGVQFHPEFKSTPWDGHPLFNAFIEAALRHQASRRAAAPKETA"
     gene            47660..48892
                     /gene="coaBC"
                     /locus_tag="PKN51_01490"
     CDS             47660..48892
                     /gene="coaBC"
                     /locus_tag="PKN51_01490"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNT83801.1"
                     /translation="MDFAGELQGRHIVLGLSGGAACFKAAEYCRLLTKAGATVQVVMT
                     EAACQFITPVAMQALSGRPVYVSQWDARPDNAMAHINLSREADAIVVAPASADFIAQL
                     AQGRAGELLALLCLARPIEQVPLLLAPAMNREMWAHPATQRNLRQVAQDGAQVLGVAH
                     GAQACGEFGDGRMLEAAELLEELIAFLAPKSLLGQRVLITAGPTFEAIDPVRGITNLS
                     SGKMGFAIARAAREAGAEVTLVAGPVHLPTPRGVHRIDVGSARQMHAAATGHAQHAHI
                     FIATAAVADWRPASAAEHKIKKDGSGQVPQLGFVENPDILADIAHSARAQAGELYCVG
                     FAAESQDLQANAQAKRVRKGVPLLVANLGPATFGKDDNALLLVDDEGVTELPRASKRH
                     LAQQLIHEIARRVSGLDA"
     gene            48889..49335
                     /gene="dut"
                     /locus_tag="PKN51_01495"
     CDS             48889..49335
                     /gene="dut"
                     /locus_tag="PKN51_01495"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNT83802.1"
                     /translation="MRLDVRILDQRLRTQMPAYATPGSAGLDLRACLDAPLTLAAGAW
                     QLVPTGMAIHLADPGYAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSAWNRSA
                     SAFTIEPMERIAQLVIVPVVQASFQIVDAFEASQRGAGGYGSTGRG"
     gene            49441..49905
                     /locus_tag="PKN51_01500"
     CDS             49441..49905
                     /locus_tag="PKN51_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNT83803.1"
                     /translation="MHLSQRFVRSLATAAAALTLAACAQHGGYPQGNYPGSYPGNQAY
                     SQFGRVTNVEYVRGGQSQGVAGAVIGGAVGGLAGSQVGGGSGRTAATVAGVIGGALIG
                     NMLERNMDRNRVEHYRVTVQFDNGGVRQFEYAQHPNVQIGDRVRAEGDQLYR"
     gene            complement(49960..50484)
                     /locus_tag="PKN51_01505"
     CDS             complement(49960..50484)
                     /locus_tag="PKN51_01505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT83804.1"
                     /translation="MEITPQCVVALTWVLKDSLGDTLDELHEPVEFLVGSEDLLPVLS
                     AALQGHESGATLNLNLEPEDAFGDFDEQLVFLARRDQLPPGIEEGMLIEAASLPAGSV
                     AQAPADALFTITEIYPEHVVLDANHPLAGMALRLQLKVVAVREALDDELERGSAGAGF
                     FRLQPMAPGSPHLH"
     gene            50540..51661
                     /locus_tag="PKN51_01510"
     CDS             50540..51661
                     /locus_tag="PKN51_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT83805.1"
                     /translation="MQIDQPLTLLGGLSPARFMQRHWQKKPLLVRQAMPGFKPLLSRA
                     ELFALAGDDAVESRLVRRSSSGWQMRRGPLARRALPPLQQADWTLLVQGVDLHHQPAH
                     ELLQAFRFVPDARLDDLMISYASDGGGVGPHFDSYDVFLLQAHGRRRWRIGRQKDLTL
                     VEGLPVKILADFRPQQEFLLEPGDMLYLPPGWAHDGVAVGECMTYSIGFRQPARDEMV
                     RELLQRVADDATDLVGEAVYRDPAQPASAQPAQLPEAMLAFARDALQCALEQPDHLAL
                     LLGEWLTEPKPQVWFEAGDGAGQVQAGVRLDRRTRMLYDARHVFINGEGFRASGTDAR
                     LMRALADARQLTAAQVGRLGADARALLQQWHRAGWLHGN"
     gene            51651..52154
                     /locus_tag="PKN51_01515"
     CDS             51651..52154
                     /locus_tag="PKN51_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83806.1"
                     /translation="METEAPPSPLPSGSFIGHESFRNHLRTGLLAAAQQGWRELLLSD
                     ADFRHWPLGEREVVEALTAWVRGARRFTMLAKSYDEVPRLHARFVEWRRLWSHRIDCR
                     VCREADATELPSALWTPGWTVQRLDLLHSSGLCGDDPARRLLLREQIDGWLVRSTPGF
                     PAYTLGL"
     gene            52340..52666
                     /locus_tag="PKN51_01520"
     CDS             52340..52666
                     /locus_tag="PKN51_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83807.1"
                     /translation="MNKSLLLAALIAAASLAACGKKEEPAPAPAPAAEAPAAPAAAAA
                     ASAVDAAASAVQGAASDAAAAVTNAASTAATAAGDAASAAAAAATDAAAAAANAAKDA
                     ASAAAK"
     gene            complement(52780..53556)
                     /locus_tag="PKN51_01525"
     CDS             complement(52780..53556)
                     /locus_tag="PKN51_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT83808.1"
                     /translation="MLRFRSLASGSAGNATLLEADDGLRRTRVLIDCGLGLRPLTARL
                     AAEGLALTDLDGVFITHEHGDHLGCARSLMRRHGVPLWTSAGTARSLDTPDAGAARPR
                     LVRAGETFAIGGIEFHPFAVPHDAREPLQLRCTDGARSLGVLTDLGHVTPQVLDALAG
                     VHALLLESNHDSELLARGDYPDFLKRRVGGTHGHLSNAQAAAALTALGHAQLTTVVAA
                     HLSERNNRPELVRQRFAAVLGCAAGEVLLAERHGTCWLGV"
     gene            complement(53581..54699)
                     /gene="bamC"
                     /locus_tag="PKN51_01530"
     CDS             complement(53581..54699)
                     /gene="bamC"
                     /locus_tag="PKN51_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNT83809.1"
                     /translation="MLNVTFIPRSCLPLLGVAVLAASGCSVLEPDRIDYKSAGRGVAL
                     EVPPDLSQLPGQSRYAAPGGSVTASAYQAGQAATVAAGTPTAANRIADVHFMRQDGER
                     WLRVDRPADKLWGDVRDFWLESGFLLAVDQPSMGLMETDWAENRAKIPQDIIRQTIGK
                     VFDSLYSTGERDKFRIRLERNPAGGTDIFVTHRGMEEVYSSARRDSTIWQPRARDPEL
                     EAEFLRRLMVRLGVSQEQSKAALAAAAAPTATPQARLVTAANGTPQVQLAEDFDRAWR
                     RVGLALDRTGFTVEDRDRSKGIYFVRYVDPSLEKQEPGFFGKLFGRGSAPLPTQQYQV
                     VVRSEGQSSQVSVLDRAGGAAPGADAQRIVKVLADELK"
     gene            complement(54767..55651)
                     /gene="dapA"
                     /locus_tag="PKN51_01535"
     CDS             complement(54767..55651)
                     /gene="dapA"
                     /locus_tag="PKN51_01535"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNT83810.1"
                     /translation="MTPITGSIVALVTPMTEDGSVDYPTLRKLIDWHIAEGTDCIGVV
                     GTTGESPTVNVEEHQEIIRVSVEQAAGRVPIMAGCGANSTLEAIELARYAKKVGADCQ
                     LQVVPYYNKPTQEGQYQHFKAIAEAVGDLPMVLYNVPGRTVGDMLHDTVLRLAQVPGI
                     VGIKEATGNIERAQWLIRDVPKGFAVYSGDDATAVALMLCGGQGNISVTANVAPRLMH
                     ELCVAAIAGDRRRAMEIQFKLMPLHKHLFVEANPIPVKWAMARLGLCGGALRLPMTRL
                     SKACEPAVEQALRDSDLL"
BASE COUNT         8187 a        19253 c        20211 g         8433 t
ORIGIN      
        1 caggaggtga aggccatcgc agccgccaga ccccgcatcg atgcggtctg cccgaggttg
       61 gcaagcggtc aactgccgtt tctaggttca atggcctgga tttgttcggt aggcgcattc
      121 agccgtcgat tgcaggctca gttgccgtcc cgtacgtggg tggcgtcgcg ctgcagcacg
      181 cccttgccga acatgggcgc cagcacatgc gccagccagc tgggccagtc caggtcgggc
      241 cggtgctggc ggtgcagcag gtgcagcgag ggcgcggcgt tcagccagcg ctcgaagtcg
      301 gcgtgcgtca tggcgtcgac gtccagcatc tggtacttga tcagcacctt ggcggcgtac
      361 agggcgtgct gcgccggctg tgcctggaac gtggcgatgc gctcgcgcgc gcggcccagt
      421 gccgcgtcga tggcgccacc cgcgcggccg aacggggcgc cgtggccggg gatgacgatc
      481 tcgggggcca gccgctcgat cacgtccagc gtctcgttga agggctcgaa accggccgaa
      541 ccgtcgatgt gcggaaagat cacgcccacg ccgtgttccc acagcgcgtc acccgacacc
      601 aggatgccgt ggcgcggctc gaacagcagc accgccaggc tgtcgtgccc cggcgcggcg
      661 tgcacctgcc agtcgtgggc gcccaggcgc accggctggc ccggcaccag cgcccgatcg
      721 gcgccgaagc gggcgcagcg ctggccggtg ccgtcgaagc tcagggcgtc ttcgtcccag
      781 cgctggaccg cgtcgagcga gggctcgggc acccaggtgg tggcggtcgg ccaggccgcc
      841 tgcagcgccc cggtgccacc gcagtggtcg ctgtggaggt gggtgtgggc gatggttgcc
      901 agcggctgcg tgcccagcac cgcgcgcacc cgccgcacgg tctgcgccgc gtgcttgacg
      961 tgcccggtgt cgacgatggc ggcctgctcg cccaggcaaa gcaggttgtt ggacgacagc
     1021 cagtcacgct gcagcacgac cagatcgtcg ggcaaggcca gcgcttgcac aggtctttgt
     1081 gacatacaaa caaaagttat cgtataactg cgccagcccc ccaattcggg gggggcggtt
     1141 tgcgcagggt gtctgcggag ccggtttgcc cgcaagggtg ctctgggtac ggggtttcga
     1201 tgcagtttat ccaacgtctt cggcgccgcc tgcgcgcgcc gtcgcttgac ctcaggcgtc
     1261 tgcggggcgc cagtcggttt ttgatgcgcc atgtcggcct ggtggggcgc gcacgctgcg
     1321 tcgccacgcg cgatgcgcat ggccacgtgg gtgtgctgct gatgatcgag acgccgcaac
     1381 gcattccgca cgaggcgcga agcgaaatcc aggcgtactt tcgtcgcaag ctgatggagt
     1441 ttggcgagct caagggccgc cccttccacc tgctgttgcg cgatcgcgac gaactgagcc
     1501 agacccagcg tgacccggtg cgcagcagtt cgtcgcgcat tgcggccatc gtggcggccg
     1561 ccaacgtggg tgtcgatgcc gtcacgctgg ccgaacaggt ggccgacagg cgcgaacagg
     1621 tgcgccagcg cctgagcgag cgccgccagg cgcgccagga aagcgcctac gtgccgctgg
     1681 cgccggtgac cgacgtcgcc ccgctggccg aagtgtgaga ggctgtgagt tctcccctgc
     1741 gcccgccttg cacgccaaga cacccccgct catcgttgca gatgcgcgtc acagcccgca
     1801 ttgtggttgc gttgggtgcc ttccgaacgg tattccacca ggaaacggcc gttggacgcg
     1861 cccacccgcg ccgtgagcgg catgccaggc aagggtgaag gccgcggcgc cagagttgcc
     1921 tgcgcgcctt gggacaacgc ggtcgggccc agcctctggc tgggctgcga cgcgcgtcag
     1981 ggacttgtgc tgcgccggcc tatgacgtga gcggccagcg ctgcgccgcc gccgccagct
     2041 ccagaccgga agtgaactgg cgccgctggc cggtgtacgg gtcgtcgaac gccagctcgc
     2101 gcgccagcag ttgcagcggg cgcgtccggt cgtccggcgc gtcgggaccg cgcagcacgt
     2161 gcggatagaa ggcatcgccc aggatcggcg cgcccagggc gcacaggtgc agccgcagct
     2221 ggtggcgctg gccgctcacc gggcgcaggt ggtagagcgc gtgtttgccc agcacggcgg
     2281 cgcgcgccac atgggtttcg ctgttgggcg cgccgggttg ctcgcgcgag atgaagaagc
     2341 gctgcggatc gggctccagc cggctgcggt gggtgcgggg ctgcgccagc tcggcccggt
     2401 cgggcgccac cgcctcgtac agcttgtgca ccgcgcggtc gcggaacagc ccctggtagg
     2461 cggcgcgggt gtgcggctgc acggccaaca ccaccagccc ggcggtttcg cggtcgatgc
     2521 ggtggatggg gctgaggccg ggcaggccca gccggcgctt caggcgcacc agcaggctgc
     2581 gctgcacgaa gcggccggcc ggcgtgaccg gcatgaagtg cggtttgtcg gccaccacca
     2641 gccagtcgtc ctgaaacacg atttgctcgg gcgcgccctc cagcgcaggc tcgtcggccc
     2701 agccgcggta gtagtacaga cgcaggccgc tgcggcaggc cagctcggcg gccaccgggc
     2761 gaccctgcgc gtccagcacc tgaccgcctt gcaggcgctg ctgccaggcc tggcgcgtca
     2821 gcgccggcag gcgttcggcc aggaagtcca gcaccgtggg ccagccggcg ccgggcacca
     2881 ccacgcagct ggggccgacg ccgtcgcgcg gcggcagcgg gggcagggcg gcgcgggcca
     2941 tgtgcgggtg ttcacgccat ctcggccagc cggcgccgga acaccaggtc ccacacgccg
     3001 tggcccagcc gcaggccgcg gcgctcgaac ttggtctgcg gccgatagtc cgggcgcggt
     3061 gcatagccgt cggcggtgtt gaccagcagc ggctcggcgc tcagcacggc cagcatctgc
     3121 tcggcgtagg gctgccagtc ggtggccaga tgcagctggc cgcccggccg caggcgctcg
     3181 gccagctcgc gcacgaaggg cggctggatc aggcggcgct tgtggtggcg cgtcttgtgc
     3241 caggggtcgg gaaagaagat gtggatgccg tccagcgtgc cgggcgcgat catgtggcgc
     3301 agcacctgca cggcgtcgtg cggcacgatg cggatgttgt ccagaccctg ctcaccgatc
     3361 agcttgagca gcgcgccgac gcccgcttca tgcacttcgc agccgatgaa cagggtgtcc
     3421 ggcatgagcg ccgcgatgtg cgccgtggca tggcccatgc caaaaccgat ctccagcacc
     3481 gctttctgat atttttggcc tccggcgctt gtctggcttg cgcaggaagc tatcaaatca
     3541 ggaacattct ggtcgtaggg caggacaaag cgcggcccca gctcggccag cgcgcgggcc
     3601 tggccgcggg tggtgcggcc ggcgcgcgtg acgaagctgc ggatggcgcg cgggtgcgtc
     3661 acgtcggcgc cggcctgcgc cgccgggccg gcggcgtccg gagggtgggc catcacggct
     3721 gcaggggtgg ggtggtggcg ggctgggccg cttcgtcggt cttgcgcagg ctcaggggcg
     3781 cgcgctgctc gccgctgggc tgcggcgcgc cgtcgaagcg cgaatgcggg cgcagcagct
     3841 gcaccagctg ggcgaacacg cgcgggttgc cggccaggca ttcgccttgc tccagaaagc
     3901 cggcttcgcc gctgaagttg ccgatcagac cgcccgcttc ggtgatcagc aggctgccgg
     3961 cggccaggtc ccagggtttg aggccggtct cgaagaaacc gtcggtgtga ccggccgcca
     4021 cgtaggccag gtcgagcgcg gcggcgccgg ggcggcgcag gccggcggtg cgctgcatca
     4081 cgtcggccat catgcgcaga tagccctgga agtcgtcgcc cttgcggtac ggaaagccgg
     4141 tggagatcag gcactcgggc aggcgcgtgc gcttgctgac gcggatgcgc cggtcgttca
     4201 ggaaggcgcc gcgcccgcgc gtggcggtga acaggtcgtt gcgcgtgggg tcgtagatga
     4261 cggcctgctc gatgcggccc ttgaccgcca gcgcgatgct gacgcagtag accggaaagc
     4321 cgtggatgaa gttggtggtg ccgtccagcg gatcgatgat ccagacatgg tcggactggg
     4381 ggttgccgcg cgtggtgccc gattcctcgg catggatgcc gtggccgggg taggcgttca
     4441 gcagggtgtc gatgatggcg tcttcgctgg cgtgatcgac ttcggtgacg aagtcgttga
     4501 cctgcttctg cgagatgcgc acggcctcga cgtcgagcgc ggcgcggttg atgatggcgc
     4561 cagcggcgcg cgcggcccgg atggccacgt tgagcatggg gtgcagggaa gacgacatgg
     4621 gttgtgggaa gagcgtgggg cccgcggcgc gcgatggcgc ggggcaacag gagacgcatt
     4681 ttcccatgct ttggcgaccg gcgccggcgc cgccgccagc accggcgcgg cacccttgaa
     4741 atcgggcgcg ccgttcacac ctgctgagca agggaggttt caacccttca tcgcatccac
     4801 cgaaaggaga ttcacatggc caaccccatc gttcgcagcc gactcttcga tgacttcttc
     4861 aacgacttcg cgcccggctt cttcgtcaag ccgctgcatg gcgatccgct gccgacggcg
     4921 gcgcagatcc gcatcgacgt gaaggaaaac aacggcacct acaccgtcga ggccgaaatg
     4981 cccggcgtcg gcaaggacga cattcacgtc tcgatcgacg gcagcgtggt gacggtgcgc
     5041 gccgaaatca agcagcagga cagccagtcc agcggcgagc gcgtgctgcg tagcgagcgc
     5101 tactacggtg ccgtggcgcg cagcttccag ctgccccagg acatcgacca gagcgccgcc
     5161 aaggccaggt acgacaacgg cgtgctgacg ctcaccctgc ccaagaagca ggtcagtggc
     5221 tcgcagcgcc tgaccatcga gtaagcgcca cgccaacccg ccgggctgcg ccggcgggtt
     5281 ggcctatgcg cccagaaaac ccaggtcgcg cgtgccgaac tgcccgatgg cgggcgccac
     5341 caggggcagt tcggtgtcgc tgacgccgaa ccagcgcgcc agcgtggcgg cgtactggtc
     5401 gaccgccgtg gtgggcagca ggcggccctg accgacgtct tccgggccgt tcagcacggc
     5461 ctgcggcgcc tgaccgtaga aggcaccgcc gcgcaccgcg ccgcccagca cgaaatggtg
     5521 gctgccccag ccgtggtcgg agccgtcgcc gttggacacc agggtgcggc cgaagtccga
     5581 cgccgtgaag gtggtgacct gtggccccac gcccagcgcg tccagcgcct gctgaaaccc
     5641 ggccagaccc gcgcccagct cggtcagcag cgccgggtgc cggttcatca ggttgtcgtg
     5701 gttgtcgaag ccacccagcg agaccaggaa cacctggcgc ctgggcccca gctggccgcg
     5761 cgcggcgatc aggcgcgcca ccatcttgag ctgctggttg agcgtgcggc gccgcccgtc
     5821 cacctgatcg ggcaggacgg cgtcgaactg gccgacgctg gtggccagcg cccccgccag
     5881 cacggcgtgc gagtccagcg aacgcgcggt gatgcggttg agttcgctct ccagcacctg
     5941 ggcgcgcggc tcggtgatca gcgtgcgcag cgcctggcgg caggcctggg cgccgtacat
     6001 gccgtcgtcc tgggtcacgc ccttgatcgc caccgccccg ccggcgccga tctggtaggc
     6061 cagagcgtgc tcgcccgaca ggaacaccgc gttgccggtg accgacaggc aggtgaacag
     6121 ggcgctgcct tcgttgccgg ccagggccag atcgcccagc cggccacccc agcccctgac
     6181 cgcaccttcg gcggccgagc tttgccagac cgattgctgg tcgttgtgcg agaacagctt
     6241 gggcggcaga ggcaccgagc gcgcccggta ctgctccagc gtggtgggca cgcgcagcgg
     6301 gccgacgttg agctgcaacg ccagccgctt ggcgtcgaac agcggcttga gcggcgccag
     6361 ctggggtgcc agcgcatatt cgcggccggc cggcaggtcg ctgccgctca gcacggtgcc
     6421 ggccaggttg gcgcgcgcga tggcaagcga ctgccgcgcc gtggcgtagt cggcgtggct
     6481 ggcgctgtcg tagggaatca gggtgttgcc gttgtcgttg ccgccataca ggaagacgca
     6541 caccagggcc ttgtagccgt cggcgctgaa ggccgcggct tcgcctatcg ccgccaggtt
     6601 gatggcccag ggtgcggcgc tgccggcaag gccgagctgg ccgagccggc gcaggaaggc
     6661 gcgccgggca ggggaagaca tgcggtggat ggccgtggga ttcatttctg caccaggtat
     6721 tcgggacagg ccatcaccag cgcgatggcc gaccagacgc gctggttgcg gccccagtcg
     6781 ctgtcgggct tgagggtggc gatggcgtcg cggatggtgg cgacggtggc gggcgagagc
     6841 tggccggccg ccagcagcag gttgagccgc tcgaccagcg ccgccgcgtc ctgcaccagc
     6901 gccatctcgt gcgtgtagcg ttcgacccag accttgctgg tcttgctgcc ccaggcgccc
     6961 caggcgatgg cgccctgcat gtagttgagg tagccggcca ccgagttctc gttggtgagc
     7021 tggaactccg gcgccggctg gccgcgctcg gccagcgcgg tgcccggcgg cacgtagccg
     7081 gggcggaaga agttgaacac cgaggggctg cgcagcgggc tctggccgag cgcggtggaa
     7141 gggtcggaca ggtcccacag gatccacttg ccgtcgctgg aggtggcgcc gaagctgcgc
     7201 gcccactgca cgaagcggat catgggttcg cgcagcttgc cccaggccgg gccgttgagc
     7261 ttggggtcct ggcgcgcctc cggatccagc agcacggccc gggtgacggc gcgcaggtcg
     7321 ccgcgcacgc cggcgccgtt gtcgttgaag gccgccgcca cccgcccgac ataggcgggg
     7381 ctgggcgcgc tggtgaccag gcgctgaatc agctggcggc cgatgaaggg ccccacgttg
     7441 gggtgctcga acaggatgtc gagcgcctgg cgcaggcgcg ccgtgccgtc gccggcggcg
     7501 atgctgcggc ccagcacggt cacgctgtcg cccgcgtgct gggccgggtt gagcttcatc
     7561 ggacggcgaa acggcgccgg atcggtcttg taggcaccgc tggcggcgtc gaggtcgtag
     7621 ccggtgaaga cctgagccag ctggctgacg tcggccgggc cgtaggtttc ctgcggctgg
     7681 ccgccgcgca gcgtgccgtc ggggttgagc tcgtacaggc cgatggtgaa cagctgcatc
     7741 acctcgcgcg cgtagttctc gtccggcacg cggccgctgg cgttggcctt cttgttgccg
     7801 cgtgtgttca gataggcgcc catggccggg ttgagggtga tggcctgcag cagctcgcgg
     7861 aagttgccga acgcgtgctg gttcagcagg tcccagtagg ccgccatggc gaacgagggc
     7921 cagtagccgt cgatgccgtt gcccgagacc accatgattt ccgaccacgc cagcgccacc
     7981 cgcttgcgca cggcgtcggg cgcggcgatc agctggttcc acatcatgtg gtcggcgtag
     8041 ccgccgttga agcgcaggct ttcctgttga tagccttgct cgaccagcca gtcccagccg
     8101 ctggtgtggc tgggcgcctg catctgcgcg tccagccagc gcgcgtagcc ctgcgaccgc
     8161 acggcggcga tgtcggcgtc gctggacgaa aactgcgcct gctgcagaaa gcgcgccgcc
     8221 tcggcggccg agatcggggc ggcaggcgga tccgcctgag tggcctcgcc gccgcccccg
     8281 caggcggcca ggccgctcgc cagcagcgcc gacaggccgc tcagggcggc gacgctgcct
     8341 ggcgagggcg ccgacgatgc gggctgggtg gcgggttcgg gcggggccgc gtctgcggcg
     8401 tggtcgaggg cggtgtcggg ggcgagcgtc tccatgcgtg tcgtgttctg gcgctggccc
     8461 gcaagctcct ctgacgtgag ttccccgggc tggatctccc cggcgcggat tagaccgcag
     8521 ttggccgccg tgccggcgct gcggcacgaa acgttagtta caaagtgttc atttctcgtt
     8581 gccccgagcg gtggccagga tcagctgcac cgccctgggc agggcgtcgg cagcgtcggc
     8641 cggcagcggc cggcgccagg gcatgccgcg cagccaggtg agctggcgct tggcgagctg
     8701 gcgcgtggcg gcgctgccgc ggtcgcgcag cagcgccagc ggccagccct gttccagcgc
     8761 ctgccaggcc tggcggtaac cgacggcgcg cagcgcgggc agctggggcg acagatcgcc
     8821 gcgcgcccgc agcgccgcca cctcgtcaag caggccggcg ctcagcatgg cgtcgaagcg
     8881 ctcggcgatt cgacggtgca gccaggcgcg gtcctgcggt tccagagaaa aaagcggcca
     8941 ggcgtttacc tggtgtgcgc cagcagctac gctatttgta gcgtgaaagc tggacagcgg
     9001 ccggccgctg agctgccaca cttcgagcgc gcgctggatg cgctggctgt cgccgggcgg
     9061 caggcgggcg gcgctcgccg ggtcgacacg cgccagctcg gcgtgcaggg cggcccagcc
     9121 gtgctggcgc gcgcgcgcct ccagctgggc gcgcacggtg gggtcggccg gcggcatttc
     9181 gtccaggccc tgttgcagcg ccttgaaata cagcatggtg ccgccgacca gcagcggcgt
     9241 gcgtccgcgc gcacggatct cggccatcag ccgggtggcg tcgcgcacga actcggcggc
     9301 gctgtagggc tggctggggt cgcggatgtc gatcaggtgg tgcggcacgg cggcgcgttc
     9361 ggccaggctg ggcttggcgc tgccgatgtc cagcccgcgg tagaccaggg ccgagtcgac
     9421 gctgatgatt tccaccggca accgctcggc cagcgccaac gccagcgcgc tcttgccgct
     9481 ggcggtgggg ccggcgatgc cgatcggccg caggccggca gcttcagtgg cgctcggggt
     9541 cgccatgcct tggcaccaga aaccaggcgg tgagggcgat caccgtgctc cagccgaaga
     9601 agcccagcgc cagcgcgcgc ggcgtgccgt ccatcatgcg gcccagccac aggcccatgg
     9661 gaaaggccac cgccatcatg atgaagccgt tgagcgccga ggccgcgccc gccatgtcgg
     9721 gaaacggcgc caccgtgccg ctctggctga ccggctgatg cacgccgtgg gccagcagga
     9781 acagcgcctg cggcacgaac accgcccacc agccgtacca gggccggccc cagcccagcc
     9841 aggccaggcc cgccatcagc gcgccggcgg tgaaggtgac ccagccggcc gcccacacgc
     9901 tgcgccggat gccccagcgc tgcagcaggc ggcggcaggc catggtgccg gcgatgtaca
     9961 ccagcgagcc ggctgccatc agcacgccgt agccgaagcg gctcaggccc agcgcctcga
    10021 tcagcacgaa gggcgaggtg gaaagaaagg tgaacagcgc cgcgtaggag gccgtggcca
    10081 gcagcgaata ggtgacgaag gtcgggtggc gggcaatgcg ccgccaggcc tgccacagcg
    10141 tgcccggcgc cagcgcccgg gggttgcggg cgggaatggt ttcgtgaaag cgcagcgcca
    10201 ccagcgccag cgtcagggcc ccgaacagcg ccagcgccgc cagcgccgcg cgccagtgga
    10261 tcagctcggt cagcagcccg cccaccggcg cgcaggtgac ggcgatcacg cccaggcccg
    10321 acaggccgcg cgacatcatg cgcgcgcctt ccagcggctc gtacaggtcg cgcacgatgg
    10381 cgcgtgcgca catcaccgcc gcccccatgg ccgcgccctg ggccacgcgg gcggcgatca
    10441 gcagcggcat gctgggtgcc agcgcgctgg ccagcgaagc cagcagatag gcgcccatgc
    10501 cccacagcag caccggccgg cggccaaagc ggtccgacag cggcccccac accagctgcg
    10561 agcagccaaa ggccagcagc aggccggcga aggtcagctg cacctgcgac atcgaggccg
    10621 ccagctcgtg ctgcagggcc ggcagcgccg gcaggtagac gtcggtggtg accggctgca
    10681 ggcccagcag caggcccagc aggacgacga cggtggaggc gctcatggcc gccggggcgc
    10741 gcccggggcc ggtgacggaa gcgggggaag acacgcgggc gagggtagca gatgacggat
    10801 ggcggatgac cgcgcggcgt gtcgatcgcc agggcgcggc tcaggtctgc gcggccgggg
    10861 tcaggggtcg agcgcctggg cgccgccggg cctcagcggc cgcgcaggaa cagggcgtcg
    10921 agctcgcgca ggctgagctg gcgccaggtc gggcggccgt ggttgcactg gtcgctgcgc
    10981 tcggtggctt ccatctggcg cagcagggcg ttcatttctt cgtgcgtcag gcgccggtgg
    11041 gcgcgcaccg cgccgtggca ggccatggtg gccagcagct cgtggcgggc gcgttcgacc
    11101 acggtgctgg cctcgtgctg cgccagctcg gccagcacgc tgcgcgccag cgccacggcg
    11161 tcgccggccg ccagcgcggt gggcacggcg cgcaccgcca gcgtgcgggg cgagaacgcg
    11221 gtgatctcca ggcccaggcg ggccagcacc tcggcgcagc cttcggcggt ggccacttcc
    11281 tgcgcggtgg cggcaaaggt ggccgggatc agcagcggct ggctggccac gcgggcgccg
    11341 tcgagctggc gcttgagccg ctcgtagacg atgcgttcgt gcgccgcatg catgtccacc
    11401 agcaccaggc cggcgcggtt ttcggccagg atgtagatgc cgtgcagctg cgcgatggcg
    11461 cggcccagcg gccagtcgtc gtcggccggg gcggcgggca gggcagccgc gccggcgtcg
    11521 ggtgcggcct gccacagggc ctgcagctcg gccacggcgt ggcccgcagg tgctttgaaa
    11581 tcgatagctg cttgtgctga tctggcgggc gctggggctg aaaatggctt gaataactcg
    11641 ggtgctggcg cgggcggtgt cgggtcggcc gtccgggcca gcagggcggc gcgcggggtt
    11701 gccagcgcgt gctcgattgc gtggcgcacg gcctggtgca cttcgcggct gtcgcgaaag
    11761 cgcacctcga tcttggtcgg gtgcacgttg acgtccaccc gcgccggatc gaccgccacg
    11821 tgcagcacgt acaccggctg gcgctggccg tgcagcacgt cttcgtaggc ggcgcgtgcg
    11881 gcgtgggcaa tcaccttgtc gcgcacgaag cggccgttga cccaggcgta ctgctggtcg
    11941 gcgcgggcgc gcgcgcgatc gggcaggccg gcgtggccgc tcacgcgcac gccgccggcc
    12001 tggtgatcga tggccaccga gccggccagg aagtcttcgc ccagcacgtc ggccaggcgc
    12061 tgcgccaggc cggcggcgcc cgggtgactg cgccattgct cgatcagccg gccttcgtgc
    12121 cagagagcaa agcccacgtc gggtcgcgcc agcgcgtggc ggcgcagcgc ctcgatgcag
    12181 tgcgccagtt cggtggcgtc gctcttgagg aacttgcgcc gcgccggcac ggcaaagaac
    12241 agctcgcgca cctccaccgt ggtgccgacg gcgcgcgcgg cggggcgcag ttcgccgctg
    12301 cgggcgtcca gctgccaggc gccgggctgc tcggcggtgc gcgacagcaa ggtcagctcg
    12361 gcgatggagg cgatggccgc caacgcctcg ccgcgaaaac ccatggtcgc caccgactcc
    12421 agctcggcca ggctggcgat cttgctggtg gcgtggcgcc tgagggcgat ggccagctcg
    12481 tccgcagcga tgccggcgcc gtcgtcctcg acgctgatca gtcgtacgcc gccggccagc
    12541 agccgcaccg tgatctggct ggcgccggcg tctagcgcgt tgtccagcag ctcgcgcacc
    12601 accgaggccg ggcgctcgat cacctcgccg gcggcgatct ggctgatcag ctcgtcgggc
    12661 agttcacgga tcgggcggcg cggcgcgggc atgggcgcga ttgtagaaag cgcgccccgg
    12721 ccaccccctg ggcttcggtg gacgccccgt atgctatgaa tagaatagct gttggcgcag
    12781 attgggcaag cgccagagcc ggtttttact tgtagagcag gttgggcagc cagagcccga
    12841 tcgaggggaa cacgtacagc agcacgatgg ccagcacctg gatcgccatg aagggcatca
    12901 tgcccaggaa gatctggttc agcgtcacgt agggcggcgc cacccccttg aggtagaagg
    12961 ccgacatggc caccggcggc gacaggaagg cggtctgcag gttcagcgcc accagcaggc
    13021 cgaagaacag cggatcgacg ccaaagtggt tcagcagcgg gatgaagatc ggcatgaaga
    13081 tgacgatgat ctcggtccac tccagcggcc agccgagcag gaagatcacc acctgcgcca
    13141 gcaccatgaa ctccagcttg ctgaggttca tcgacagcac ccactgctcg atcagctcct
    13201 gcccgcccag cagcgcaaag gcggccgaga agatggccga gccgacgaac agccagcaca
    13261 ccatggccga ggtcttggcc gtcaggtaga ccgactcgcg cagcacgtcc atggtcagcc
    13321 gcttgtaggc cgccgccagc agaaagccgc ccagcgagcc catggccgcc gcctcggtcg
    13381 gcgtggccag gccgaacacg atggagccca gcaccgccag aatcaggatc atcagcggga
    13441 agaacgagcc cagcagcatc ttgaagattt ccagccgggc gaagctgagg aacaggtaga
    13501 acagggcggt gcaggcgccc agcaccccca tcacgaccca ccaccaggtg ggcgccggct
    13561 gcgtgccggc ctgggctgcg ccggcggcat cggaggccgc ggcgctggcc ccggggggct
    13621 cggccacgcc ggcggccgtc ggctcggacg cggcatcgac ctcggcgccg ggcggggtgg
    13681 ccagggtgtc gggcgcatcg gcccctggcg gctcctgcag ggcgtcctcg gtcggaggtt
    13741 cctgcaactc atcttcggcc ggcggctcgg ccagcccgga gtcggagctt tcggcgccgc
    13801 tgccctccga tccgatggcc tgcagctcca ccgcctgctc ggccggcacc ttggtcacgg
    13861 cctgccaggt gctggtggcc agcagcagaa acagcagacc cggcagcacc accaccgcca
    13921 gctggcgcag cacgtgggcc agcggcacgt cggcgttgcg ccggcccttg agcgcgttca
    13981 gcaggcgcag cggcgcgtag tcgcgcgtgc cagccggcag ggcgctggtc agggtcggca
    14041 gatcgacctg gcgctcttcg gccgacagcg gcggcgccca ggcaggcttg agcttggcca
    14101 cgatgatgac gtaggccaca tacagcccgg ccagcatgac gccggggaag aaggcgccgg
    14161 catacagctg caccaccgac accccggccg tggcgccgta caggatgagc agcaccgagg
    14221 gcggaatcag aatgcccagg cagccgccgg cggtgatggc gcccgccgac atggggatgc
    14281 tgtagcccgc gcgcagcatg gccggcagcg ccagcagccc catcagcgtg accacggcgc
    14341 cgacgatgcc ggtggccgtg gcgaagatgg cgcaggtcac cagcgtggcc accgccagcg
    14401 agcccggcac gcgcgccatg gccaggtgca ggctcttgaa cagcttttcg atcaggttgg
    14461 cgcgctcgac caggtagccc atgaagacga acagcgggat ggcgatgagc acgtcgttgg
    14521 ccatgctctt gtaggccgcc tgcaccatca ggtcgagcgt gcgcgtgacg ttgcggtcgt
    14581 acgccagcca ggtgaagatc atgcccatgc ccatgagcgt gaacgcggtg ggaaagccca
    14641 gcatgatggc caccaccacc agcgacagca tcagcaggcc caggtggccg ttggtgatgt
    14701 gctccacgcc cagcagcagc caggcggcgc tggccaccac cagcgccatg atggaaaagc
    14761 cgaaccagag ttccttgcgc atgctcaggc cccccggtcg gtgtgcccgt ggtccgcgtg
    14821 cccggggctc ggcggcgacg gtcggggcgg ctgcaccgcg atggcggcgc ggtcggcgtc
    14881 ggtgacgtgc accatttcct tgagtttttc cacgtccact tcctcgacgt cggcctcgcg
    14941 gctgggccag gcaccctggc gcaggcagat cacgcagcgg gcgatctcgg ccagaccctg
    15001 cagcagcagc gccgcgccgg ccagcggaat gacgaacttg aaggggtaca gcggcagcgg
    15061 ctcggccgag aaggtctgct cgcggatcgc cagcgattcc tgcgcatagc cccagccggc
    15121 ccaggtcatg gccagcacgc cgggcaggaa gaacagaaag tacagcagca ggtccagccc
    15181 ggcctgcacg cgcgggctga agaagccgta cagcacgtca ccgcgcacat ggccgttctt
    15241 ggccagggtg taggcgccgg ccatcatgaa catggtgccg tacagcatga tctgcgcgtc
    15301 cagcacccag gcgtggggct tgttgagggc gtagcgcgaa aacacttccc agcagatcag
    15361 ggccgtgagc agaacgcagg cccaggcggc ggccttgccg atgaaggtcg acacggcgtc
    15421 gatcccgaga agcaactttt gcatggcgaa acagccgcga gggccgatga aaacgaacag
    15481 ggcccggcga tcgatttcgc ccagggcccc gggtcgacaa aaaagaaggc cttgcgcagg
    15541 ccttctcgaa tgcgggcggc gtcagcccat ggcgcccttc ttggcgccga agtaatggtt
    15601 ccaggccatg cggtagtcga tggtggtgtc gttctgccag cgcccgctgc gctgggcaaa
    15661 ggtgcgcatg gaatccatga ccttcttgaa catcgggttc tcggccgcct tcttggccgt
    15721 caccttgtcc caggcggcga gctgcgcgcg caggatggcg tcgggcgtct tgtagaactt
    15781 cacgccctgc ttggccgaca tctcggcgta gtccttggcg tagcggtcgg cggccttcca
    15841 gctcatgtcg gcgctggcgg cctgcgtggc gtagtcgatg atggccttga gctcggccgg
    15901 cagcgcgtcg tacttggtct tgttgaacag cacctcgaac tgttcgctgg actggtgaaa
    15961 gctctgcagc atgcagttct tggccacgtc ggggaagccc agcacgcggt cgctcgacgc
    16021 attgttgaac tcggccgcgt cgagcaggcc gcggtcgagc gccggaacga tctcgccgcc
    16081 gggcagggga ttgacggcca cgcccatctc cgtgaacacg tccacggcca ggcccacggt
    16141 gcggaacttc aggcccttca ggtcttccac cttggccacg ggcttcttga accagcccag
    16201 cggctgcgtg ggcatggggc cgtagagctt ggagaccacg tccaggttca ggctcttgta
    16261 gatctcgtcg agcagctcct tgccgccgcc gtagttgtgc caggccagca ccatgttggc
    16321 gtccatgccg aaggccgggc ccgagcccca gagcgccacg gcctggttct tgccatacca
    16381 gtaggccacc acgccatggc cgccgtcgag cgtgcccttg gccacggcat cgagcagctg
    16441 aaaggccggc accacggcgc cggcgggcag cacctcgatc ttcagacggc caccggccat
    16501 gtcgttgatt ttcttggcgt agtcctgtgc gaactcgtgg aagatgtcct tggccggcca
    16561 ggtgctctga aagcgcaggg tggtggtctg cgccacgctg atcatgggca ccgacatggc
    16621 gccggcggcg acggcggcgc ccttcagcag gttgcgacga ccggtggtct gggtgggttt
    16681 gctcatcttc ctctgtctcc gggtgggttg gtgtctggac agtccagagg ggtgatgatc
    16741 cgatcgcaag cccttgtgtt caaagagggt tttcaccgag ggcgcggccg tggtgcgcga
    16801 aaaccacgcc tgccgggggg cgcggcgtgc cgccggcgcg tcgctgggcg ccggataatc
    16861 ggggccatgg atgtcctgat gatgctggtg gacttcgtgc tgcacgtcga tcgccacctg
    16921 ggcgagttcg tggcggccta cggggcctgg gtgtacgcgc tgctgtttct gatcgtgttc
    16981 gtcgagaccg gcctggtggt gatgccgttt ctgcccggcg attcgctgct gttcgtggtc
    17041 ggcgccctgg ccggggccgg gcacctgagc tggccgctgg cctgcgcggt gctgctggcg
    17101 gcggcgatcc tgggcgacca gtgcaactac agcctggggc gctatttcgg gccgcgcgtg
    17161 ttcaagtggg aaagctcacg cctgttcaac cggcaggcgt tcgaccgcgc gcaccggttc
    17221 tacgagcgct acggcggcgt cacggtgatc ctggcgcgct tcatgccgtt catccgcacc
    17281 ttcgtgccct tcgtggccgg cgtggccgag atgacgcgcc accgcttcac gctgttcaac
    17341 gtcgccgggg cgctgatctg ggtgctgggg gtggtgagcg cgggctacct gttcggcaac
    17401 ctgccctggg tgcaggccaa cctgagcaag atcatctggg ccctgatcct ggtgccgggc
    17461 ctgatcgcgc tgtacggcgg ctggaaggcg ggccgcgccg cgcccagcgc ctgatcaggt
    17521 atcaaaaccg gctctggcgc gctaccggat tgcgccggaa gctatcaaaa caatagtatt
    17581 cttgacgccg tttccagggt cacaggcggg ccggggacgc tgcctgcccg tggcgtgacg
    17641 cctcaccccc tgggtcatcg aagcgcagcg cacgcacccg ccatctgcac cacctcagac
    17701 ctgccggtgg cgcgccagcg gcgggttggc ggtgaagtac ttgcggatgc cgctgatcag
    17761 ggcgtcggcc agctcgttct ggtaggcctg gctgcgcagg cggcgctctt cgtcggggtt
    17821 gctgatgaag gcggtctcga ccagcacgct gggcacgtcg gggttgcgca gcacggcgaa
    17881 gttggcgcgc tcgacgctgc gcttgtgcag tctgccgacg ccgccgatct cgcgcagcac
    17941 cgtgtcgccc aggcgcaggc tgtcgcggat ctgcgccgtg gtgctcatgt ccagcagcac
    18001 gtgctgcacc gactggtcct tggcgcccag gctgacgcca ccgatgcggt cggaggcgtt
    18061 ttccttttgc gccagccaac gcgcggcggt gctggaggcg ccgcgctcgg acagcgcgta
    18121 gacgctggcg ccgctggcct cgggccgcat gaaggcgtcg gcgtggatgc tgacgaacag
    18181 gtcggcctgc acccgccgtg ccttttcgac ccgcgtctgc agcggcacga agtagtcgcc
    18241 ctcgcgcgtc aggaaggcgc gcatcgggtt gccgcccacg ctgctggcgt tgatgcgctc
    18301 gcgcagcttc agcgccacct gcagcacgac gtccttttcc tgcgtgccgc cagggccgat
    18361 ggcgcccggg tcctcgccgc cgtggcccgg atcgagcgcc acgatgatca ggcgctcggt
    18421 ggcgccggcg ccggccggcg gtggggcagg gcggcgcgcg gcgtcgcgcc gggtgagttc
    18481 gttggcgcgc tcgatctggc gcgcgatcag ctcacccagc gtgtcggtgc cgccgtgcgc
    18541 gggcgcggcc gccggctcga ccggcaccgg cgcggggctg cggccgagct cgcgcatgcg
    18601 ctcggcgatg agctgggcca gcgggtcggg cgggtgcgcc gggtacagat cgaacaccag
    18661 ccggtgctgg taggccgcca ccggccccag ggtgaacacc tgcggcttga tctcgtgctt
    18721 gaggtcgaac accaggcgca ccacgccggg ctggtgctgg cccacgcgga tgccggcgat
    18781 gttggggtcg tcggcgcgca ccttggccac cagctcgcgc agcgtggggt cgagcgtcag
    18841 gccgtcgatg tccaccgcca gccgcggcgg cgtcccgacc aggcggtagc tggccttgag
    18901 cgcggtgtcg gattcgacgg tgacgcgcga atagtcttcg gccggccaca cgcgcaccgc
    18961 caggatgccg gctccgcgcg ccaactggtg cgcgcccagc cacagcacca gcgagccgcc
    19021 ctgcagcagt gcgcggcgcc gcccgggtgc cggtggccag ccgggcgact cgggggtgac
    19081 cggcgtcatg cgcccagctc cgtcagcagg cgccgacccc gcggcgtgtg ggcgctgacg
    19141 cgcacggcgc gggcgccatc gtcgcgcacc gtcagccgca gcgccaggtc gggcgccggc
    19201 agcaggccgg cggccttgtc gggccattcg gacagcttca gcccgggcgc ggcgaacagg
    19261 tcgcgcagac cggcgtcttc ccactcgcgc gggtcggtga agcggtagaa gtcgaaatgc
    19321 gacaccgcca gcccgtcggg cgtctggtgg ggttcgacca cggcataggt cgggctcttg
    19381 atgcgcccgg caacgcccag ggcgcgcagc aggtggcgca cgaagctggt cttgccggcg
    19441 ccgagctcgc cgtgcagctc gatgcaggcc tggcgcagct cgggcgcggc cgccaggcgg
    19501 cgggcaaagg cggcggtgtc gtcctcgctg cgccacagca ggctgagggc gggcgcatcg
    19561 gggcttccta caatttgggc gtggctggag atggcgctca aatcgattcc cttgatctgt
    19621 tgaaccagtt gcgcggctgg gcgcgcgagc tggggttttc gcacctgggc gtggccgaca
    19681 tcgacctctc ggctgccgag cccggcctgc tggcctggct gcacaacggt tttcacggcc
    19741 gcatggacta catggcggcg cacggcctgc ggcgcgcgcg cccggccgaa ctggtgcccg
    19801 gcacggtgag cgtgatcacc gcgcgcatgg attatctgcc gcgcgccacc cccgaggact
    19861 ggcaggacgt ggagctggcg cggctgggcc gcccgcagga gggcgtggtg tcgctctacg
    19921 cccgcgggcg cgactatcac aaggtgctgc gcgggcgcct tcagaagctg caggaccgca
    19981 tcgcgctggc catcggcccg ttcggccacc gcgtgttcac cgattcggcg ccggtgctgg
    20041 aggtggagct ggccagccgc agcggccagg gctggcgcgg caagcacacg ctgctgctgt
    20101 cgcgcgaagg cggctcgatg ttctttctgg gtgaaatcta cgtcgacctg cacctgccgc
    20161 gcaccgcacc gaccggcgcc cactgcggcc agtgcagcgc ctgcatcgac gcctgcccga
    20221 cgggcgccat cgtggcgccg taccggctgg acgcgcgccg ctgcatctcc tacctgacga
    20281 tcgagcacga cggcgccatc gacgaggcgc tgcgcccgct gatcgccaac cgcatctatg
    20341 gctgcgacga ctgccagctg gtgtgtccgt ggaacaaata cgccgggcgc agtgcgctgc
    20401 ccgatttcga cccgcgtgag ggcctgggcg gcgcccagtt gaccgatctg ctggactgga
    20461 gcgaagacga gttcttgcgc cgcaccgaag gcagcccgat ccgccgcatc ggccacgagc
    20521 gctggttgcg caacgtcgcc gtggcgctgg gcaacgccct ggccgcgctg ccgcccggcg
    20581 acgcgcggcg cgcgcccctg cacgccgccc tgaggcgccg cgccaaccac ccgagcgcgc
    20641 tggtgcgcga gcatgtgcgg tgggcgctgg ggcgttgaca tcggggcgat cactatggtt
    20701 tttgccggct cgcatggcgc gggatcggcg ccgcatgctg ctgtatttat agcatttcgt
    20761 cgggcgcgtc aacccgcgat cggcgtggcc aggcgcagac ctcgaacaag agggggtcgc
    20821 cgcgcgcgcc agcaaggcgc cggcagcctc gcttcaaggg gcaacccgct ccagcagcgt
    20881 gaacgcccag ggcaggctca gcagcgccag cagggtcgac agcgtgacca gccccgccac
    20941 gtaagggccg ttgtagccca tgcgcgcggc cagcacgtag ccgctggacg aggtgggcag
    21001 ggcggcaaac accagcagcg ccgccgcttc ggccgcgccc aggccaaagg cgcgcgccag
    21061 cagccaggcg atcagcggtt gcagcaggtg gcggatggtc agcagcgcgg tgcccagcag
    21121 cttggcgcgc gccaggctct gcagctgcat gccggcgccg gccgccatca gcccgagcgc
    21181 gatcgacgcg gagccgatgc gcgacagcgt ggtggcggcc cagtccggca ggccgaggcc
    21241 cagcaggttg gccaccagcc ccagcacggt ggccaggatc agcgggttgc gcagcagctc
    21301 gccgccgatg gcgcggccgc tgcggcgcgc catcggccac accgcggcca cgttgtagag
    21361 cggcacgctg accccgatca gcaccgccat caactgctgg ccctgggcgc cggccaggcg
    21421 ctcggccacc gccaggccga tgaaggaatt gaagcgaaac gcgatctggg cgctggccgc
    21481 gtggtcgtgg gcgtcgatgt gccgccccag cagcggcaga tgcggcagcg cccaggccag
    21541 ggcgatgccg gcggcgccca gggccaggcc ggcgccgatc aggctggatg cggcctgcac
    21601 gtccagcggc gcgcgcacga tggactgaaa caggtagacc ggaaacagaa agaaatacac
    21661 cagttgttcc accgcctgcc agacgccacg gcccagggcc gtgtggcgac acagcagcca
    21721 gccgagcgca atcagcgaaa aatcgggcag cagaagcgtg acgtggtgca cggcgcgagc
    21781 atagcggcct gcgcaatccc taatggccag gccggcggcg gcatggttaa catggcgcgc
    21841 ttttttatcc ttcaaccact cagaggaacc ggaccgccat gcaacgacgt caatggatcg
    21901 ttctcgccgc tgccaccctg tccgccggcg cggtgtgcgc gcaggcctac cccagcaagc
    21961 cgattcgcct ggtggtgccg ttcgcacccg gcggcaccac cgacatcatc gcccgcacca
    22021 tcgccgaccc gctgggcaag gcgctggggc aggccgtggt ggtcgaaaac aaggctggcg
    22081 gcggcggcgt gatcggcgcc accgaagtgg tgcgcgccgc ccccgacggc tacaccctca
    22141 gcgtggccac ggtgtccacc acggccgcca acccggccat caacaaaaag attccgtacg
    22201 acccgatcaa cgacttcgtg ccggtgatca acatcgccgc cacgcccaac gtgatcgccg
    22261 tgcaccccag ctttccggcc aaggactacg cctccttcgt cgcactgctc aagaaggcgc
    22321 caggcaagta ctcctacgcg tcctccggca ccggcggcat cggccacctg cagaccgagc
    22381 tgtggaagag cctggccggt gtgttcgtca cccacattcc gtaccgcggc gccggcccgg
    22441 cgctcaacga caccgtggcc ggccaggtgc ccatcatctt cgacaacctg ccttcggcgc
    22501 tgcccttcat caagggcggc aagctggtgc cgctggtggt cgccgcgccc gaacgcctgg
    22561 ccgatctgcc caacgtgccg accttcaagg aaatcggtct gccggcggtc aaccgtctgg
    22621 cctactacgg catcctggcg cccaagggca cgtccaagga gatcgtcgac aagctgaacg
    22681 ccgccacccg caaggtgctg gaagatccgg cagtcaagaa gcgcatcgcc gacaccggct
    22741 ccatcatggt ggccaacacg cccgagcagt tcgcccagca gatcaaggac gagttcgccg
    22801 tctacaagga ggtggtggcc aagcagaagc tgacgctcga ttgatcggcg ccggcccgcg
    22861 accggacacg cccgcaggtc ccaggccggc ggcgtgcccg gatcgcagcg gacttgggtt
    22921 gctattatta acgtagctgc tggcgattga tgggcgcgtg ccagagccca atatgacacc
    22981 gaatccggcg ctcgcatcga tgcccattgc atcgcgccga cccatggccg ctgccgcacc
    23041 cgacgacccg ctgatcgacc gcttcatcga cgccctgtgg ctggaagacg gtctgtcggc
    23101 caactccctg gccgcttacc ggcgcgacct caccctgtac gccgcctggc tgcaggccac
    23161 gcaccgcgtg gcgctggcgc agacccagga gccgcacctg aacgcctaca tggccgcgcg
    23221 cgccgacggc aaggccacct cggccaaccg gcggctgacg gtgttcaaac gcttttttcg
    23281 ctgggcgctg cgcgaacacc tggtgagcgc cgacccgacg ctcaggctgc tggccgcgcg
    23341 ccagccgctg cgcgtgccca agacgctcgg cgaggcgcag gtcgatgccc tgctgaccgc
    23401 gcccaacacc ggcaccccgc tcggcctgcg cgaccgcgcc atgctggaac tgatgtacgc
    23461 cagcggcctg cgcgtgagcg aactggtggc gctcaggagc tttcacgtcg gtttcaacga
    23521 aggcgtgctg cgcgtgctgg gcaagggcag caaggagcgc ctggtgccgt ttggcgagca
    23581 ggcgcgcgac tggctggagc gctacctgcg cgaggcccgt ccggcgctgc tgggcgggcg
    23641 ccagagcgat gcgctgttcg tcaccagcgc cggccgcacg cccggcaccg cgatgtcgcg
    23701 cgtgatgttc tggaacctgg tcaagcgcta tgcgcgcgcc gccggcgtga cggcgccgct
    23761 gtcaccgcac acgctgcgcc acgccttcgc cacccacctg ctcaaccacg gagccgacct
    23821 gcgtgcggtg cagctgctgc tggggcatgc cgacatttcc accaccacca tctacaccca
    23881 catcgcacgc cagcggctga aggaattgca cgcgcagcac catccgcggg ggtgagggca
    23941 agtgcctgag cgtggtgcga tgcatcaacc tgcataatcc cctgctgacg aaaggggaaa
    24001 ggatcgtgac agtcagcgaa tcgagcggat gcgccgctgg actgcgtgtg gctgtggtga
    24061 cgccgtattt caaggagtca ccggcgacgc tgcagcgctg cttgaccagc gttcgcgccc
    24121 agacctaccc gcaggtggtt cactatatgg tggccgacgg ctttcctcag aacgccttgc
    24181 tgacgcagtg gccaggcgtg cgccacatcc agctgccgaa cggccacgcc aatttcggct
    24241 gtaccccgcg aggtgtgggc gcccagtgcg cgctggccga tggtttcgat gtggtgtgct
    24301 ttctggacgc cgacaatctg atcgagcccg agcatgtcgc caccgtcgtg gaaaccgttg
    24361 ccaaggcgca ggctgccggg gttgctctgg acgcggtgtt ttcgctgcgg tacctgttct
    24421 tgccgggtca cgaacatctg cggttggtgg cgccgcgcga agacgtgggc tctggattcg
    24481 tcgacaccag ttgcatcagt ctgacgcgct cggcgggctt tctgtggggt gcgtggtgcc
    24541 agataccgtc ctcgttgacg ccgatctgtg accgggtcat gtgctggctc atgcagcatc
    24601 atcgcctcaa ggtggcctgg actggccaac gcacggtgct gtacgaatcc agctgggcgc
    24661 acacctacgt acaggccggc gtgcccgtgc cggcgagcgg gacgcacgac cacaccttgc
    24721 gcgccgtggg tcaaggattg acgcccgaag agctgtgggc acggttgcgg gtgcgctgga
    24781 cattcgttcg acccatcgat ggcgccgcgt catgacccgt gccgtggtta ccggcggggc
    24841 cggcttcatc ggcagccacc tttgcgaggc cctgctgatc cagggcgttc aagtcacgtg
    24901 cgtggacgac ctgtccagtg gaagcagcga caacgtggcg cacctgctgg cggtccctgg
    24961 gtttgccttc gtgcctgggg acgtggtgga tccggccttg tggcagcgat tgccggctgc
    25021 ccagcgcgtg tttcacctgg ccagcccggc cagcccggtc gactaccagg ccgcgccggt
    25081 gcagaccatt cgcaccaacg tcctgggcac cctgaacgcg ctggactttg cgcgggccca
    25141 cggtgcccgc atgttgctga cttccaccag cgaggtgtac ggcgacccgc aggtgcatcc
    25201 acagaccgag gcctaccggg gccacgtcaa cccggtgggc ccgcgcagtt gctacgacga
    25261 gggcaagcgt tgcgcggagg ccctgacggt ggcgcatgcc cggcagcacg gcacccaggt
    25321 gcggctggcg cgtctgttca acacctatgg cccgcgcctg cgaccacagg acggtcgcgt
    25381 ggtcagcacg ttcatcacca gcgcgttgcg cgcagagccg ctgccgctgc acggcgatgg
    25441 tcggcagacc cgctccttct gctacgtcag cgataccgtg cgtggtctgc tggccctgat
    25501 ggcgcaggag cgcgtgacgg gtccgatcaa cctgggcaat ccgcaggagg tgtcgatgca
    25561 tgcgttggct gccatggtgc tgcgcctgac cggcagcaac gctggcgtgc ggacgctgcc
    25621 ggcacgtgaa gaggacccgc gctgtcgcca gcccgacatc agccaggcac agcgttggct
    25681 cgaatggcaa ccggaagtgc ccttggtgca aggcctggcg cagaccatcg agtggttccg
    25741 cgcgcaaggt gccgtaggtg cgaatcggtt gccgtgcggg agccagccgg tggtggatct
    25801 ggcggccaac acctgatccc cccactcagc ctggaagcgg cggttggagg ggcttgcccg
    25861 cgccgacgca gattgcggcg cgcgccggga gggtgggccg ttcgcccagt cagcgacgcc
    25921 gctcagccaa gcgccagccg cgcccggtgt cgtgcgatct gtgtctgcac ctgcgccggc
    25981 gccgtgccac ccagggtggc gcgggcgttc aggctgccgc gcaggctgag gacctggtag
    26041 acgtccttgt cgatggccgg gtggaattcc tgcagcacct tcagcggcag ctccgacagg
    26101 tcgcaggcgt gcgactgggc ggctttcacg gcgtgggcca cggtttcgtg tgcgtcgcgg
    26161 aagggcaggc ccttcttgac aaggtagtcg gccaggtcgg tggcggtggc gtagcccttc
    26221 tgcgccgccg cctccatggc ggcggggttc acgctgatgc cgccttcctt ggcgccgctg
    26281 accgggttgg gctggccgcc gatcatctcg acgaagatgc gcagggtgtc gcgcagcgtg
    26341 tccacggtgt cgaacagcgg ctccttgtct tcctggttgt ccttgttgta ggccagcggc
    26401 tggcccttca tcagggtgat caggcccatc aggtggccga ccacgcggcc ggtcttgccg
    26461 cgcgccagtt cgggcacgtc ggggtttttc ttctgcggca tgatcgacga gccggtggtg
    26521 aagcggtcgg cgatgcggat gaaggcgaag ttctggctca tccacagaat cagctcttcg
    26581 ctcagacggc tgatgtgcac catcaccagg ctggcggcgg cggaaaattc gatcgcgaag
    26641 tcgcggtcgc tcaccgcgtc caggctgttc tggcagacgc catccatgcc cagcgtgcgc
    26701 gccacgcgct cgcggtccag cgggtaggtg gtgccggcca gggcggcgct gcccagcggc
    26761 agctggttga cgcgctttcg ggcatccagc atgcgctgtt cgtcgcggct gaacatctcg
    26821 acgtaggcca gcaggtggtg ggcaaagctc acgggctggg ccacctgcag gtgggtgaag
    26881 ccgggcagga tgacgtcgat gtggcgctgc gcgacctcca ccagcgcgcc ctgcagctcg
    26941 cgcagcaggc cggtgatgag gtcgatctcg ccgcgcagcc acaggcggat gtcggtggcc
    27001 acctggtcgt tgcggctgcg gccggtgtgc aggcgcttgc cggcgtcgcc caccagctgg
    27061 gtcaggcgcg cctcgatgtt caggtgcacg tcttccagcg ccagctgcca gctgaattcg
    27121 ccgcgctcga tctcgccctg gatctgcgcc atgccgcgcc tgatgtcggc caggtcctgg
    27181 gcgctgatga tgccctgcgc cgccagcatg tcggcgtgcg ccaggctgcc ggtgatgtcg
    27241 gcgctggcca ggcgccggtc gaagtcgacg ctggccgtgt agcgctgcac cagctcgctc
    27301 atcggttcag agaacagggc ggaccaggcc tgggatttct tgtcgagttg gttggcggac
    27361 atgcgggtgt gaagaagtgg cgcgcggact gacgaacggc gcggctggac ggacggacgc
    27421 gcccggagcg cgcgggttgc ggcctacact gcggccacaa tcgccacggc ccatgcagga
    27481 ctcgcaaatt ctatcgacct tcggtgaacg caagcggccg cccaaggcgc gcccggcgcc
    27541 gccaccgctg gtgttcgacg cctgtcaggt gggggtgatc ctgcgcgccg tgctgtacgt
    27601 ggaggcggtg atcggggtcg ggctgatgta cctcaccgcc gacctgtcgg actggctggc
    27661 gcgggtggcg ctggttaccg gcggcgccct gccggccacg ctgctgtggc tggtgctggc
    27721 ctgcgcgctc aagcgcctgc tggcgcgcct gagctggccg ctgcaggccg ccgccggggt
    27781 ggcgctgggg gcgctggccg gcttgtacgc ctgcggcacg ctggcgctgc tcggttttgc
    27841 cgccgcgccg ccctggctgg ccagcgccag caccggggcg ttgctggccg ccggcctgat
    27901 ggccgggttg ctgtggcgcg tgcgcggtcg ccagccggcg gccaccacgg cgcggctggc
    27961 cgagctgcag tcgcgcatcc gcccgcattt tttgttcaac accctcaaca ccgccatcgc
    28021 cctggtgcgc gccgagccgg ccaaggccga acgggtgctg gaagacctgt ccgacctgtt
    28081 ccgccacgcg ctggccgagc agggcgacag cgccaccgtc ggccaggaaa tcgagctggc
    28141 gcagcgctat ctggagatcg agcaactgcg tttcggcgag cgcctgcggg tggagtgggc
    28201 gctggacgag cgcgtgcacg gcgcgcgcct gccgccgctg atcctgcagc cgctggtgga
    28261 aaacgccgtc aagcacggcg tcgagcccag ccccggcggc gccgagctgc gcatttcgac
    28321 ccagcggcgc ggccagcgcg cgctgatcaa ggtcaccaac accgtgccgg ccgggcaggg
    28381 gcgggcgggc ttcggcatcg cgcaggccaa cgtgcgcgac cggctgcgcc tgctgcacga
    28441 tctggagggc gacttccgca cggcgctggt ggacggcgta tatcaggccc gcatcgaggt
    28501 gcccttgccg gggcgagggg cgtgatggcg ctgcaggtgc tggtggtcga cgacgagccg
    28561 ctggcgcgtg cgcgcctgcg cgcgctgctg gccgactgcc gcgagcccga ggctgtggcc
    28621 gccgccgagg ccggcacggc cgcccaggcg ctggccctgg cgcagcacca gcgcttcgat
    28681 gtggcgctgc tggacatcca catgccgggg gccgacggca tgcagctggc ctgcgcgctg
    28741 cgcgccttgc ccgagccgcc gcgggtggtg ttcgtcaccg cccacgccga acacgcggtg
    28801 cgcgccttcg agatcgatgc cgtcgactac ctgaccaagc cggtgcggct cgaacgcctg
    28861 caggcagcgc tacaaaaaac gcagcgcgtg gcgcagttgc agagcgcgcc agagcccgat
    28921 tcggcccctg actggttgct catcaccgag cgccaccgca cgctgcgcgt gccgctggcg
    28981 gaggtgctct acctgaaggc cgagctcaag tacatcaccg tgcgcacggc cgacgccagc
    29041 catctgctgg acggcagcct gaaccagctg gaagagcgcc acggcgggcg ctttctgcgc
    29101 atccaccgca gcacgctggt ggcgcgcaac gccatccggg ccctggtgcg ccaccaggac
    29161 ccggacgagg gcgagggctg ggccgtgcag ctggccggcc tcgacgaagc gctgccggtg
    29221 tcgcgccggc agctggcggc ggtgcgtgcg gcgctcggcg agccgcgctg aacggcgcgc
    29281 cggccgtcgc cggcggccgg gcgcacaggg cacgccgctg aggtctcgcg ggtgccgggc
    29341 cgcggcgtcc ggtagcatcg ccccccatgt ctctttccat caccatcgcc acccgtgaaa
    29401 gccgcctggc cctgtggcag gccgaacatg tgcgtgccct gctgacgcag cgcggccaca
    29461 gcgtgcggct gctgggcatg accacccagg gcgaccagat cctcgaccgc acgctcagca
    29521 aggtgggcgg caagggcctg ttcgtcaagg agctggaaac cgcgctcgaa gacggccgcg
    29581 ccgacattgc cgtgcactcg ctcaaggacg tgccgatgga gctgcccgcg ggcctgcagc
    29641 tggcctgcgt gatggcgcgc gaggacccgc gcgacgcctg ggtgtcgccc gcgcacgcca
    29701 gcctggatga gctgccggcc ggcgccgtgg tcggcacctc cagcctgcgc cgcacggtgc
    29761 tgctgcgcgc cctgcggccc gacctgcgca tcgagccgct gcgcggcaac ctcgacacgc
    29821 ggctgcgcaa gctcgacgaa ggccggtacg ccggcatcgt gctggccgcc gccggcctca
    29881 agcgcctggg gctggcggcg cgcatccgcc acatcttcga cagcgacgcc atgctgccgg
    29941 cggccgggca gggggcgctg ggcatcgaaa tccgcgaagg ccgcggcgag ctgacgcagg
    30001 cgctggcgcc gctggccgac cagccgacct ggctggcggt ggcggccgaa cgcgccgtca
    30061 gccgcgccat ggggggcagc tgctcgatgc cgctggcggc gcacgccacg cggcagggcg
    30121 agcgtctgca cctgcgcgct gcctggggcg accctgaagg cgcgccggtg ctggtcaggg
    30181 ccgagggcga ggcccaggta agcaccctgg cgcaggccga agccctgggc gagcgtgtgg
    30241 cggccgggct gcgcgccggc ggcgcccgtt gagccggccc atggctgcgc cgcgcgtgct
    30301 cgtcacccgg ccggcgcacg acgccgccca ctgggtgggc cagctgcgcc aatgcggcat
    30361 cgacgcgcag gcgctgccgc tgatcgacat cgtgcccgag cccctggccg gtcgcctgct
    30421 gcaggcgcgc cagcacctgg gcgactatgc cgtggcgatg ttcgtcagcg gcaacgcggt
    30481 caccgggttt atggggtcaa atcaggctgc agtgcagttg cagcaagcgc tgacagctat
    30541 cgaaacaaga gcatggtcgc ccggtccggg caccacggcg gcgctgcagc gggccggctg
    30601 gccggccgct cgcatcgatc agccggcgcc cgaggcgccg caattcgact ccgaatcgct
    30661 gtgggcccag gtggcaccgc aggtgcgccc tggcctgcgc gtgctgatcg tgcgcggcgg
    30721 tgacgccgcg ggcagcgtgg ccggccgcga ctggctggcg gcgcagctga cggcggccgg
    30781 cgccgaggtc gatcaggtgc tggcctaccg ccgcacggcg ccgcggctgg atgccgccgg
    30841 cctggcgcgg gccacggcgg cggcgcgcga cggcacgctg tggctgttca gcagctccga
    30901 agccattgcc cacctgcgcc agtgcctgcc gcagatcggc tggcaggcgg cgcgcgcgct
    30961 ggtcacccac ccacgcatcg gcgccgcggc gcgggccgcc gggtttggca cggtgcacca
    31021 cagccagccc acgctggacg cggtgagtgc gtcgataaaa tcgctcgcat gacgtccgag
    31081 ccgagccccg cagcgcctgc cgcgccgccc cctgtgccgg cgcccgtggc gaccgaggcc
    31141 ggcacggcgg cgccggccag cccgctggcg ccagcgcatg ccgcggcacc gcgcggcgcc
    31201 ggcgtgtccc ccggcctgtg gctggcctgg ctgctggccg cggccggcct ggtggtcggc
    31261 ctgctgctgt ggcagaagct gaccggcatg caggaacagc tggcgcgcca gagccgggaa
    31321 gccggcacgc agtcggtgga ggcgcgcacc ctggcgcgcc aggccgagga gggcgtgcgc
    31381 gacatggccg gcaaggtggc ggcgctggac gcccgcctgg ccgaactggc cgcctaccgg
    31441 gcgcagctcg acgagctggt gcacagcgtc gcccgcgcgc gcgacgagaa cctggcggtg
    31501 gatctggaag ccgccctgcg cctggcgcag gaccagaccc agctgaccgg cagcgtggag
    31561 ccgctgctgg ccgcgctgcg caccgccgag cggcgcctgg cgcgcagcag cgacccgcgc
    31621 ctggcgccgg tcggccgcgc cgtggcgcgc gacctcgagc gcgtcaagtc ggccagcatc
    31681 cccgacgccg ccggcctgct ggcgcggctc gatcagctgc tgcggcaggt cgacgatctg
    31741 cccgccgcca acgaggtgcc gcacgcgcgc agcgccccgg ccaccaccgc ccccgccgcc
    31801 gccgccgaaa gcgcctgggg ccggctgtgg cagagcgtgc gggacgaggc ccagggcctg
    31861 ctgcgcgtca gccgcatcga acgccccgag gccagcatgc tcagccccga gcaggtgttc
    31921 ttcgtgcgcg aaaacctcaa gctgcgcctg cagggcgcgc gcctggcgct gctggcgcgc
    31981 cagtacgaag ccgcgcgcgc cgacctgacc gccgccgcca tgtccctggg cacctggttc
    32041 gacccggcct cgcgccgcac ccaggccgcc gccaccttgc tgcagcaggt ccaggccagc
    32101 acccgctcgg ccgagctgcc gcgcgtggac gattccctgg tcgccctggg gcaggccgtg
    32161 gccacggccc acacggtgcc cgggacgcgc tgaccaccat gcgcgccgcc ctgtggttga
    32221 tgggtctgtt cgccgccgcg gtggcgctgg cgctgtttgc cggtgacaac gaaggcacgg
    32281 tgacggtgtt ctggccgccg caccgggtgg acgtgtcggt caacctggcg ctgctgctgc
    32341 tgctggcgct gttcgtgctg ctgtacctgg cgctgcgggc gctgtcggcc atggttcagc
    32401 tgccgcgcca ggcgcgccgc tggcgcaccc agcagcgcga gcgcgccatg cacgccaccc
    32461 tgatcgacgc tgccgcgcaa ctggccgccg gacgctttct gcgtgcgcgc aaggccgccg
    32521 aaagcgcgct gacccgggtg caggccctga gcgacgccgc cgaagccccg ccgcacgcgc
    32581 ccagcctgcg caccctggcc cacatgacgg tggccgaaag cgcgcacgcc ttgcgcgacg
    32641 aggccatgcg cgaccagcat ctggaacacg cgctcgacgt ggccggccgt gccggcgccg
    32701 cccccgagct gcgcgaaggc gtgctgctgc gcgccgcgcg ctggtcgctc gaccagcggg
    32761 acgccggcgc ggcgctggcg cggctcaacg agctgcccgc cggtgccacc cggcgcacgg
    32821 cggcgctgcg catcaagctc aaggcggcgc gccagagcgg gcaccacgcc gaggccctgg
    32881 aaaccgcgcg cctgctggcc aagcacgggg ccttttcgcc ggccgcggcg cgcagcctgc
    32941 tgcgcgggct ggcggccagc ctgatcgacg aggtgcacga cccggcgcag ctgcaggccg
    33001 tgtggggctc gctggacgcc ggcgagcgcg ccatgcccga gctggcgctg cgcgccgccc
    33061 agcgcatgct ggcgctggac ggcccgcgcg ccctggtgcg gcaatggctg cgcccggtgt
    33121 gggagcaggc gctggcccag ccaggcagct tcggcccggc gctgcgcgcc cgcctggtgc
    33181 aggtgctgga gcaggccctg atcgccaccg gcgaggcctc cgaccagcaa tggctggcgc
    33241 tggtcgagtc ggcccagcag gcccacccgc gcgaccccag cctgcaatac ctggccggca
    33301 tggcctgccg ccaccgcggc ctgtggggcc gggcgcagct gctgctgggt caggcggcgc
    33361 agtcgctgcc cgacgccgag ctgcgccgcc atgcctggcg cgcgctggcc gagctggccg
    33421 agcagcgcgg cgacgagccc gccgccgcac aggcctggaa gcaggcggcc cggtcctgac
    33481 ggccggcgcc accggcgggg ggggggctgt attccaagga taatcggcct ccagcgcttg
    33541 tctggcaagc gccaaaagct attgaataaa tagcaaatcg ggtgccgacg gcggtccgaa
    33601 gccactcccc gggatcctgc cgtcatcgac ccgcagcgag gccatccgtc accgatgcat
    33661 gccggttggc gtcagaccca gcccgaggcc tgcaattcct tcttgccgta ggcgtagaac
    33721 agcggcgccg ccaccagacc ggccggaccg aacacggctt ccatcacgaa catcacgctc
    33781 agcagctccc acacgcccat atgggtgcgg tggccgatga ccttggcgtt gatgaagtat
    33841 tcggccttgt ggatggcgat cagaaaggcc aggcaggcca gcgccacccc gggcgacacc
    33901 gacaggccga ccagggtcag cacgccgttg cagatcaggt tgccgacgat cgggatcagg
    33961 ccggccacga aggtcagcag gatcagcgcc atcgtgtagg gcaggcggtg gccggtgagc
    34021 ggcaggatcg ccagcaggaa gacggcggtc aggcaggtgt tgaacagcgc gatccagaac
    34081 tgcgccacca cgatctggcg aaaggtctcg ccgaagcgcc gcacgcgcag gtgcaactgg
    34141 gcggccagcg gcttgaggcc cggcgtgctg gggcgggccg ccgccagcgc gccgatcagc
    34201 agcccgacgt aggcaaacag caggcccgac agccaggcgc gcccggtggt ggccagcgtg
    34261 ccggccttgc tggccaggta gctggccatg gcgcgctgga tctcgaccgc gccttcgggc
    34321 agctgggcgc cgatgtccgg cggcagccgg tcgcgcagct ccagcacggt gcgcgccaga
    34381 aagcccagca gctcgcggta ctgggccggc gcgtccagaa tcagcccgcg cgtgcgcggc
    34441 agcgccagcg ccagcagcag cagcggcagc agcgccacca cggtggcggc caggcgcggt
    34501 gcctggcggt cggcctggcg tgccggcagc gccagccgtg ggctgagcca gcgggtgcag
    34561 aaaaagccca cgcacacgca cagcaggccc ggcagcaggc cctgccacat gaccagcagc
    34621 aggctggcgg cgatcagccc gtggctggcc agggtggcgg tgcgcaggcg cgggtcgggc
    34681 aggggcgcgt gcatcggctg caaggtcgat cgggtagggg gtggccggct tcaggccacc
    34741 tgcacggcgg cgccgtcgcc gcgcgcggcg atgtggccga tctcgaacac ctgctcgccg
    34801 gccgcgcgca gcgcctgggc acaggccgcg gcctgcgccg gcgccaccac gaccaccatg
    34861 ccgatgccgt tgttgaaggt gcggttcatc tcgtggtcgt cgatgccggc ggtggcctgc
    34921 agccaggtga acagctcgct gcgcggccag ctgccggcgc gcaggtgggc ggccaggcct
    34981 gcgggcagca cgcgcgggat gttttcgagc aggccgccgc cggtgatgtg cgccagcgcc
    35041 ttgatgccgc cgggcgccgc cagcgccgcc agcaccggct tgacgtagat gcgggtgggc
    35101 gccatcacgg cctggcgaaa cggctgcccg tccagggtgg ccggcaactg gccggcggcg
    35161 cgctcgatgc acttgcgcac cagcgagaag ccgttgctgt gcacgccgct ggaagccagg
    35221 cccagcacca cgtcgccggc ggccacgtcg cgcccggtga ggagagcgga cttttcgacc
    35281 gcgccgacgc aaaagcccgc caggtcgtac tcgccgggcg ggtacatgcc gggcatttcg
    35341 gcggtttcgc cgccgatcag ggcgcagccg gccagctcgc agccgcgcgc gatgccgccc
    35401 accaccgtcg ccgccgtgtc cacgtccagt cgcccgcagg cgaagtagtc gaggaagaac
    35461 agcggttcgg cgccctgcac cagcacgtcg ttgacgctca tggccaccag atcgatgccg
    35521 acgccgtcgt gcatggccca ttcgaaagcc agcttcagct tggtgccgac gccgtcggtg
    35581 cccgacacca gcaccggctc gcggtagcgc cgcggcactt cgaacagcgc gccgaagccg
    35641 cccaggccgg acagcacccc ttcgcgcagg gttcgccggg ccagcggctt gatgcgttcg
    35701 accagcgcat cgccggcgtc gatgtcgaca ccggcatcct tgtagctgag gggggagggg
    35761 gtggaggagg ggctcatcga ggctgggcgg ttgaaaatgg cgggcggtcc gcgcgcggga
    35821 catgcgctgc gtagaatcga ggccagattc taggcgctgc acccccgtgc ccctgaccgc
    35881 gccccgcctg tttccggctt catgcacccc ctgttcatgc cgtttttcgc ccctggcgtc
    35941 cgatgaggca gatcgccctg gacatcgggc tggcgcccga acccaccctg agcggctttc
    36001 tggccggcgc caacgccgcc gccgtgcagc acctgcggct gtggacgggt ggcgccacgc
    36061 gctcgccggt gccgacctac ctgtggggcc cgcccggcag tggcaagacc cacttgctgc
    36121 gcgcggcgcg ccaggcgctg gccgagcagg gcgcgcgggt cggctggctg gaccccgaaa
    36181 ctccgccgcg cctgcccttc gacgaggcct gggatgccgt gctgatggac gacgtgcagc
    36241 ggtacggcgc cgagctgcag cacaccgcct tcaactggtt cgtcaacgcg atcacgccgc
    36301 ccagcggccg gccgcgcgcc gtgctggcgg ccggcggcgt gccaccggcc gacctgccgc
    36361 tgcgcgagga cctgcgcagc cggctcggct ggggccatgt gttcgagctg cacccgctgc
    36421 ccgagccgga gctgcgcgcc gcgctgcgcc aggccgccga cgcgcgcggc gtgttcctgt
    36481 ccgacgaggt gatggacttc gtgctgcggc gctttgcacg cgatctgggc agcctgatgc
    36541 aactgctcga ccagctcgac cgctacgccc tgcaggccca gcgcgccatc accattccgc
    36601 tggtcaaggc gatgctggaa aatgaatagc aggcggcgcc ctgtccgcct gaaccaacca
    36661 ctgattcgat gctcaaactc gccctgttcg acctcgacca cacgctgctg ccgatcgatt
    36721 cggactacgc ctggggcgag ttcaccaccc gcatcggctg gaccgatccg gtcgacttcc
    36781 agcgccgcaa cgacgagttc tacgcccgct accaggccgg cacgctggac gtgcgcgagt
    36841 acgtgcgctt tgccaccgaa gcggtgcgcc gccagggccc cgagcaggcg gcccgggcgc
    36901 atgcgcgctt catgcgcgag gtggtgggcc cggcgctgcg cccgcaggcc cgcgcgctga
    36961 tcgaggcgca ccgccaggcc gggcatggcc tgctgatggt gacggcgacc aacgaattcg
    37021 tcacacgacc gatcgcccag gcgttcggca tcgaagagct gatcgccatc gagctggtgc
    37081 gcgatggcgc cggctggatc accggcgaaa tcgccggcac cccctcgttt cgcgaaggca
    37141 aggtggcgcg cgtggccgac tggctgcgcg cccgcgggca cgattggagc gacgtcgaca
    37201 tcaccttcta ttccgactcg cgcaacgacc tgccgctgct ggaaaaggcg cagcagccgg
    37261 tggccaccaa cccggacgac gcgctgcgcg ccatcgccag cgagcgcggc tggcgcgtgc
    37321 tggagctgtt caaggaagac acatgatcaa gcaactgatc cacaagctga tcgacagcgc
    37381 caccggaggc aacgcgcgca agcggcgcgg caagcgggtc gaaatcggcc ccgagggcca
    37441 cggcatcaac ccggcgctgg tggacgagcg tgccgccggc gtggtgcgca cgctcaagga
    37501 agccggctac gaggcctaca tcgtcggcgg cgccgtgcgc gatttgctgc tggggctcaa
    37561 gcccaaggac ttcgacgtcg ccaccaacgc cacgccggag caggtcaagc agctgttccg
    37621 gcgcgccttc atcatcgggc gccgctttcg catcgtgcac gtggtgttcg gccgcgggcg
    37681 cgagcacgag gtgatcgagg tgtccacctt ccgcgcctac gtggacaacg ccaccgccga
    37741 ggccatccgc ggcaacgaac gcaccgccaa gggccagctg gccggcatga agcacgcggt
    37801 agacgccagc gggcgggtgc tgcgcgacaa cgtctggggc agccaggacg aagacgcggc
    37861 gcggcgcgac ttcaccgtca acgccatgta ctacgacccg caggcgcgcg tggtggtgga
    37921 ctaccacggc ggcatcgccg acgcgcacaa gaagctgctg cgcatgatcg gcgatcccgc
    37981 cacgcgctac cgcgaagacc cggtgcgcgc catccgcgcc gtgcgctttg gcgccaagct
    38041 ggcggcgctg ggctttcgta tcgaacccaa gaccgcccgc ccgctggtcg aagtcctgcc
    38101 gctgctggcc gaggtgccgc aaagccgcct gttcgacgaa atgctcaagc tcctgcagac
    38161 cggccacgcg ctggccagcg tcgagcagct gcgcaagctg gggctggcgc gcggcatcta
    38221 cccgctgctg gacgtggtgg tcgagcgcgc cgaccagccc ttcgtgaagg cggcgctgca
    38281 agacaccgac cggcgcgtgg gcgaaggcaa accggtggcg ccctcgttct tgctggcctg
    38341 cgtgctgtgg caggacgtgc tggcgggctg gaacgcgcgg ctggcgcgcc aggaacaccc
    38401 gttcccggcg ctgcaggacg ccatcgacga ggtgttcgac gcccgcgtcg gtgacgtgtc
    38461 cgggcgcggc aagctgggcg ccgacatgcg tgaaatctgg atgatgcagc cgcgctttga
    38521 aaagcgcagc ggccagacgc cgttcagtct ggtcgagcag ccgcgctttc gcgccggctt
    38581 cgacttcatg cgcctgcgcg ccgacgtggg cgaggtgagc gaggagctgg ccgactggtg
    38641 gcagcagttc agcctggccg acgacggcga gcgcgaaagc atgatgaccg agctgcgtgc
    38701 cgagcagcag cgcagcaaac gccaggcgcc gcgcgtgcac cgggtgccgg cgggcacgct
    38761 ggagacggcg ccacccgcgc ccgcccccga cgccgccgcc accccggcca agaagcgccg
    38821 gcggcgccgt cgcaagcctg ccggcgcggg cggatcggcg cccgaggccg gcccgccgcc
    38881 ggcccagccg tgacgcagcc gctgtcccat ctgcgcgcgc cggtgctggc ctatgtggcg
    38941 ctgggcgcca acctgggcga cgcggccgcc agcgtgcgcg ccgccatcga ggcgctggcg
    39001 cggctgccgg acacgcagct gcaggcgcgc tccagcctgt accgcagcgc gccggtggac
    39061 gccggcgggc cggactacgt caacgccgtg gtggcgctgc gcaccggcct gtcggcgccc
    39121 gagctgctgg ccgggttgca gcggctggag caggcggccg gccgccagcg gccgtatcgc
    39181 cacgcgccgc gcacgctgga cctggacctg ctgcgctatg gcgacggccg catcgacagc
    39241 gctgcgctgt gcgtgccgca cccgcgcatg ctgcagcgcg ccttcgtgct gcgtccgctg
    39301 gccgagattg cgccgcagct ggtcacgcca gcggcgctgg cggccgtggc cgggcagggc
    39361 atcgaacgtc tggagggcgc atgagccctg ggcggtccat gccggattcg gagccaaacc
    39421 gggctccagc gcatatcaat actgcgccag cagctatgat tattgtagct tttcgaacgc
    39481 gacaccgggt gcggccgacc gcccggcggt gccaaggcgt ctcagcggtg gggcagtggt
    39541 tgatttgcca cgtgtgtcag ccagcgccgc agtcggggtg gttaaactca ccggatcgtc
    39601 gtgggcgctg cgatccagtg gtccgcagac tttcgattta ctgaagcaag gttatccctc
    39661 atgctgctca gcaagctgtt gcccaaggag ggcaattttt ttgagctgtt caaccagcac
    39721 gccgaccgca tcgtcgaagc cgccaatgcc ttttcgctgc tggtggcgca ctacggcgac
    39781 ctgggacagc gcgaaaaata cgccagggac gtggacaacg ccgagcgcgc cgccgaccgc
    39841 gtgaccgaag aggtcaaccg cctcatccac accaccttca tcaccccgat cgaccgcgag
    39901 cagatccacg gcctgatcaa cctcatggac gacgtggccg acctgctgca ggactcggcc
    39961 gagacgatgg cgctgtacga catccatcac atgaccgagg aaatcgtgcg cctgaccgag
    40021 ctgtcggtca agtgctgcca gcgcgtgcag gccgcggtgc gcctgctggg caagatcgcc
    40081 gagccgacca cggccgaggc cacgctcaag acctgcgagg agatcgaccg cctcgaatcc
    40141 gatgccgacc gcgtcatgcg cagcgccatg agcaagctgt tccgtgaaga gccggacgtg
    40201 cgcgaggtca tcaagctcaa ggccatctac gaactgctgg agaccatcac cgacaagtgc
    40261 gaggacgtgg ccaaccagat cgagggcatc gtcctcgaaa actcctgagc cgagcgcgca
    40321 gccatggaaa cggtgcaggc cgcgctctgg gtcgtcgtgc tgctggtcgc gctggcgatc
    40381 gtgttcgact tcatgaacgg ctttcatgac gccgccaact cgatcgccac ggtggtctcg
    40441 accggcgtgc tcaagcccac ccaggcggtg gtgtttgccg ccttcttcaa cttcatcgcc
    40501 atcttcatct tccacctcag cgtggccgcc accgtgggca agggcatcgt gcagccgggg
    40561 gtggtggaca cccacgtggt gttcggcgcc ctggtggggg ccatcagctg gaacgtgctg
    40621 acctggtact acggcatccc cagcagctcc tcgcacgcgc tgatcggtgg catcgtcggc
    40681 gccaccgtgg ccaaggccgg caccggcccg ctggtgatgg ccggggtgct caagacggtg
    40741 gccttcatct tcgtgtcacc gctgctgggc tacctgctgg gctcggccat gatggtggcg
    40801 gtggcctggg tgttccggcg tgtgcggccg gggcgggtcg acaaatggtt tcgcagcctg
    40861 cagctgctga gcgccggcgc ctacagcctg ggccacggcg gcaacgacgc ccagaagacc
    40921 atcggcatca tctggatgct gctgatcgcc accggctatg ccggcgccgg tgaatccagc
    40981 ccgccgctgt ggtccatcgt gagctgctac gccgccatcg ctgccggcac catgttcggt
    41041 ggctggcgca tcgtcaagac catggggcag aagatcacca agctcaagcc ggtcgggggc
    41101 ttttgcgccg aatcgggcgg ggcgctgacc ctgttcatcg ccaccgggct gggcatcccg
    41161 gtgtcgacca cgcacaccat caccggcgcc atcgtggggg tcggctccac gcagcgcgcc
    41221 agcgcggtgc gctggggcgt ggccggcaac atcgtctggg cctgggtgct gaccataccg
    41281 gccagcgcgc tggtggcggc gctggcctac tggctgagcc tgctgctgtt ctgaacgcgc
    41341 tatgtgttca atagctggtg gcgcagtctg ggcgctattg agcgcctgat tgttcgcttc
    41401 acgcgctact gcgaaatctt gcgcgcttcc tcgatctggt actcgaagta gcgctgaaag
    41461 ctgaacgcca gcgaggccat cagcacaccg gcgccgatca gcagcgccac cgagatgccg
    41521 aacaccgtgc cccaatgggt ctggccgggc gcggcctgcg ggtcggcctg ggggttgaag
    41581 cgggcgttcc agtgctcggc gctcatcagg ccgtagacga tggcggtcag cgcgcaggcc
    41641 gagatggtga agcccagcag cgggatcagc agccagctca gagggtcgtc ctggccgaac
    41701 tgctgcacgc gctcgatgcc gtacaggccg agcgcggtcg ggatcggcag caaccagccc
    41761 agcaggtcgc caaagcccct cagatagaag cggtgcaggc ccagggggcc gccgagaaag
    41821 gccagccagg tggccagggt cttgtttttc atgcggttcg gcaaacggtg tcggacgggc
    41881 gcccgcgccg tgcgggccgg ggtttctggg cgccgccggc ggcggccagc ctcggggggc
    41941 agggcgcagc ggcggcagcc gctataatca taggcttttc ggtgtgtcgc tggccgggtg
    42001 gccatgtcgc gtgtctcagc accggaagaa cctgggcctt gcgcgctttt gccccaacct
    42061 gaagcgccga ggcccgagcc accacccaca ggatttcagt tcaacatggt cgttattcgt
    42121 ctctcgcgcg gcggcgccaa ggcccgtccc ttctacagca tcgtggttgc cgacaagcgc
    42181 gcgcgccgcg acggcagctt catcgagcgc atcggtttct acaaccccgg cgccgcccag
    42241 ggcgaagagg gcctgcgcat cgcccaggat cgcctgacgc actggctggg cgtcggcgcg
    42301 cagccgtcgc tcacggtgca gcgcctgatc aagcaggccg ccaaggccgc tgtggccgcc
    42361 gcctgagcgc gcccgcccgc atggtcccgg ggctggagcc ggccgagctg ccggcggacg
    42421 cggtcgaagt cggccgcatc ctggacgcct ggggcatcca gggctggttc aaggttcagc
    42481 cctacagcgc ctcgcccgag gcgctttttt cttgcaaacg ctggtggctg ctgccacccg
    42541 agcgcgcgcc caagcccgcc tggcccggcg ccgtggcgct gaacatccgc caggcgcgcg
    42601 cccacaccga tgccgtggtc gccagcgcgg ccgacatcac cgaccgcgac gctgccctgg
    42661 gcctcaaggg cgcacgcatc ttcattccgc gttcggcgtt tccggcaccg gccgaaggcg
    42721 agtactactg ggtcgatctg atcggcctga ccgtggtcaa ccgtgagggc ctggtgctgg
    42781 ggcaggtgag cgacctgctg gccaccggcc cgcagcaggt gctggtggtc ggtcaccagg
    42841 aggccggcaa ggcgcgtgag cgcctgatcc cgttcgtcga cgcctatgtc gacgaggtcg
    42901 acctgccggc ccggcagatc agggtcgatt ggcagcccga ctactgaacc tggccacgct
    42961 ggccgcaggc gctcatcgtc agcgcggcgc cacctgcacg aagatttcgt tgggcttgac
    43021 catgcccagc tcgatgcggg cgcgttcctc gaccatgccc agcccggcgc gcaggtcttc
    43081 gacctcggac gccagctgct cgttgaccag ccgcgcctgc tggttggcgc tgcgttgctg
    43141 atccagcttg cgttgcagcg ccgacacgtt gggcaggctg ccgcgcccaa tccacagctg
    43201 ggcgtggatg acgaccagca ggagcagcag gatggccggg acgacgcggg aggtcatggc
    43261 agcatggcgc agggtgcaag cggattcgtt gctatctttt tagtatagct ccgaaccgcg
    43321 ccggcacgct gccaggacgc aggccatgcc gacccgccga cgtgccgcag cgcgccgcat
    43381 cacttcaggt tgtagaaagc ggcgcgcccg gggtacctgg ccatgtcgcc cagatcctct
    43441 tcgatgcgca gcagctggtt gtacttggcc atgcggtccg agcgcgacag cgagccggtc
    43501 ttgatctggc ccgcgttcag gccgaccgcg atgtcggcga tggtcgagtc ctcggtctcg
    43561 cccgagcggt ggctgatgac ggcggtgtag ccggcgcgct tggccatctc gatggcgtcg
    43621 aacgtctcgg tcagggtgcc gatctggttg atcttgataa ggatcgagtt ggcgatgccc
    43681 ttgtcgatgc cttccttgag gattttggtg ttggtgacga acaggtcgtc gcccaccagc
    43741 tgcaccttgt cgcccaggcg ctcggtgagc agcttccagc cgtcccagtc gccctcggcc
    43801 atgccgtctt cgatgctgat gatggggtat ttgtcgaccc aggtggccag catgtcggtc
    43861 cattgcgcgg cgctcagttt caggccgcct tcgccatcga gcacgtactg gccgtccttg
    43921 aagaactcgc tgctggcaca gtccaggccc agcacgatgt cctggccggc ggtgtagccg
    43981 gcgtcctgga tggcctgcag gatcagctgg atggcggcct cgtggttttc gacgttgggg
    44041 gcaaagccgc cttcgtcgcc caccgccacg ctcatgccct tgtcgtggat gatcttcttg
    44101 agcgcgtgga acacctcggc gccccagcgc agcgcttcgc ggaaactggg cgcgccgacc
    44161 gggatgatca tgaattcctg caggtccagg ttgttgttgg cgtgtgcgcc gccgttgatg
    44221 acgttcatca tcggcacggg gagctggcag ccgttcatgc cgccgaagta gcggtacagc
    44281 ggcaggccgg attcttcagc cgccgcgcgc gccaccgcca tcgacaccgc cagcagggcg
    44341 ttggcgccca ggcgactctt gttgtcggtg ccatccagat cgatcagggt cttgtccaga
    44401 aaggcctgtt cggccgcgtc caggcccagc acggcctcgg agatttcggt gttgatgtgc
    44461 tcgacggcct tgagcacgcc cttgcccagg tagcgcttct tgtcgccgtc gcgcagctcg
    44521 atggcttcgc gcgagccggt ggaagcgccg ctgggcacgg cggcgcggcc catcacgccc
    44581 gactccagca gcacgtcgca ctcgacggtg gggttgccgc ggctgtccag cacttcgcgg
    44641 ccgacgatgt caacgatggc actcatggtt ttccttttcc gaaagtttca ataaaaatcg
    44701 ggctctagcg caataccagc aagcgccagc agctatcaaa ctaatagcat cagacgccct
    44761 cgacgcagag caggcgcatg acggcggcgc cctggcgggc ggcgcgcgcg cggctgtact
    44821 cgggtgagtc gtgaaaggcc ttggcggcct cgaagctggg gaacttcagg atcaccgtgc
    44881 gccccgggtg ccagtcgccc tccagcacct ccacccggtc tccgcgcacg cagatttcag
    44941 cgccgtgcac gcgcatggct tcgctgctcc agcgcttgta ttcctcgtac tgttgcgggt
    45001 tggtgatggt gaccagggcg atgatgtagc cggcgggcat ggttcaggct ccgaagtcgt
    45061 cttccagaaa gccctggcgc ttggtgacct gatcgagcgc caccagcgtc tccagcagcg
    45121 ctttcatgtg cctgagcggg accgcgttgg gcccgtcgga cagcgccctg gccgggtcgg
    45181 gatgggtttc catgaacagg ccggccacac ccacggcgac cgccgcgcgc gccagcaccg
    45241 gcaccatctc gcgctggccg ccgctggcct ggcccagacc gcccggcagt tgcaccgaat
    45301 gcgtggcgtc gaacaccacc ggcgcaccgg tttcacgcat gatggccaag ctgcgcatgt
    45361 ccgacaccag gttgttgtag ccaaagctgg cgccgcgctc gcaggccatg aagcggtcgg
    45421 tgtccaggcc tttttcagcc gccgccgcac gcgccttgtc gatcacgttg accatgtcgt
    45481 gcggcgccag gaactggccc ttcttgatgt tgaccggttt gcccgactgc gccacggcgc
    45541 ggatgaaatc ggtctggcgg cacaggaagg cgggtgtctg cagcacgtcc accacggcgg
    45601 ccacggtggg cacctcggcc tcggtgtgca cgtcggtgag caccggcacg ccgatctcgc
    45661 gccgcacgcg ggccaggatc tgcaggccgg cgtccatgcc cgggccgcgg aagctggcac
    45721 cgctggagcg attggccttg tcgtagctgc tcttgaagat gaaggggatg cccaggctgg
    45781 cggtgatttc cttcagctgg ccggccacgt ccatctgcaa ctgctcggac tcgaccacgc
    45841 aggggccggc gatgaggaag aacggacgcc gcaggccgac gtcgaagccg cacagcttca
    45901 tgccgtctcc ttgggcgcgg cggcgcggcg gctggcctgg tgccgcaggg cggcctcgat
    45961 gaaggcgttg aacagcgggt ggccgtccca gggcgtggac ttgaactcgg ggtggaactg
    46021 cacgccgacg aaccacgggt gcacgccctg cggcagctcg acgatctcgg tcagttgctc
    46081 gcgctgcgtg agcgccgaaa tcaccaggcc ggcggcgcgc aggcgctcca ggtagtccac
    46141 gttggcttcg tagcggtggc gatggcgctc ggtcaccacg tcgccgtaga tgcggtgcgc
    46201 cagcgtgccg ggcaccacgt cggcgctttg cgcgcccagg cgcatggtgc cgcccaggtc
    46261 ggagccggcg tcgcgccgct gaatgcggcc gtccttgtcc ttccattcgg tgaccagcgc
    46321 gatgaccgga tgcctggtgg cagggttgaa ctcggtgctg ttggcgcctt ccagtccagc
    46381 cacgtggcgc gcgtattcga tggtggccac ctgcatgccc aggcagatgc ccaggtaggg
    46441 cagcttgtgc tcgcgcgcgt actgcacggt ggcgatcttg ccttcgaccc cgcgctcgcc
    46501 aaagccgccg ggcaccagga tggcgtcgaa gcgcgccagc tgcgccgccg tggcgggcgt
    46561 gatggtctcg gaatcgatgt gctcgatgtg cacgcgcgca tggttgtgca tgccggcgtg
    46621 gcgcagcgcc tcgttgaccg atttgtagct gtccgacagg tcgacgtatt tgccgaccat
    46681 ggcgatcgag acctcgtgcc gcgggctttc gacctcgtgc accagggcat cccagcgctt
    46741 gaggttggcc ggcggcgtgt tcaggcgcag cttgtcgcag atcaggccgt cgaggccctg
    46801 ctgatgcagc aggcgcggca ctttgtagat ggtgtcgacg tcgggcatgc tgatgacgcc
    46861 ccaggccggc acgttggtga acaggctgat cttctcgcgc tcgtcctgcg gaatggtgcg
    46921 gtcggcgcgg cacagcaggg cgtcgggctg gatgccgatt tcgcgcagct tctgcaccgt
    46981 gtgctgggtc ggcttggtct tgagctcgcc ggcggcggcg atccagggca ggtaggtcag
    47041 gtgcacgaag gcgctgtggt tgggccccag cttcaggctg agctgacgca ccgcctcaag
    47101 gaaaggcagc gattcgatgt cgcccacggt gccgccgatc tcgacgatgg ccacgtccac
    47161 cgcgtcgggc gtgccgacgc cggcaccgcg ccggatgaag tcctggatct cgcccgtgat
    47221 gtgcggaatc acctgtaccg tcttgcccag gtagtcgccg cgccgttcct tctcgagcac
    47281 gctctggtag atctggccgg tggtgaagtt gttggccttc ttcatccggg tggtgatgaa
    47341 gcgctcgtag tggcccaggt cgaggtcggt ttcggcgccg tcgtcggtga cgaacacctc
    47401 gccgtgctgg atcgggctca tggtgcccgg atcgacgttg atgtacggat cgagcttgat
    47461 cagggtgact ttgaggccgc gcgattccag gatcgcggcc agggaggccg aggcgattcc
    47521 cttgcccagg gaagacacca caccgccagt gacgaagacg aatttggtca tgtcaggggc
    47581 gaaggccgca acctccgctg ggtgggaaat ttcaattata ggagccggac cctctgctac
    47641 attgcgcgcc atgaatgcca tggacttcgc gggggagctg cagggccgcc acatcgtgct
    47701 gggcctgtcg ggtggcgcgg cctgcttcaa ggcggccgaa tactgccgcc tgctgaccaa
    47761 ggccggcgcc acggtgcagg tggtgatgac cgaggcggcc tgccagttca tcaccccggt
    47821 ggcgatgcag gcgctgtccg gccggccggt gtacgtgtcg cagtgggacg cgcggccgga
    47881 caacgccatg gcgcacatca acctcagccg cgaggccgac gccatcgtgg tggcgccggc
    47941 cagtgccgac ttcatcgccc agctggcgca gggcagggcc ggcgagctgc tggcgctgct
    48001 gtgcctggcg cggccgatcg agcaggtgcc gctgctgctc gccccggcca tgaaccgcga
    48061 gatgtgggcc catccggcaa cgcagcgcaa cttgcggcag gtggcgcagg acggcgcgca
    48121 ggtgctgggc gtggcgcacg gcgcgcaggc ctgcggcgag ttcggcgatg gccgcatgct
    48181 ggaggcggcc gagctgctgg aggagctgat cgcctttctg gcccccaagt cgctgctggg
    48241 ccagcgcgtg ctgatcacgg ctgggccgac gttcgaggcc atcgacccgg tgcgcggcat
    48301 caccaacctg tccagcggca agatgggttt cgcgattgcc cgcgcggcgc gcgaagccgg
    48361 cgccgaggtg acgctggtgg ccgggccggt gcatctgccg accccgcgcg gggtgcaccg
    48421 catcgacgtc ggctccgcac gccagatgca cgcggcggcc accggacatg cgcagcatgc
    48481 tcatattttc atagcgacgg cggcggtggc ggactggcgc ccggccagcg ccgccgaaca
    48541 caagatcaag aaggacggct ccggccaggt gccgcagctg ggcttcgtcg aaaaccccga
    48601 catcctggcc gacatcgccc attcggcgcg cgcccaggcc ggcgagctgt actgcgtcgg
    48661 cttcgcggcc gaaagccagg acctgcaggc caacgcccag gccaagcgcg tgcgcaaggg
    48721 cgtgccgctg ctggtggcca acctcggccc ggccaccttc ggcaaggacg acaacgccct
    48781 gttgctggtg gacgacgaag gggtgacgga gttgccgcgc gcctccaaac gccatctggc
    48841 gcagcagctg atccacgaga tcgcgcgccg cgtctcgggg ctggacgcat gaggctggac
    48901 gtccgcattc tcgaccagcg tctgcgcacg cagatgccgg cctacgccac gccgggcagc
    48961 gccgggctgg acctgcgtgc ctgcctggac gcgccgctga ccctggcggc cggcgcctgg
    49021 cagctggtgc ccacgggcat ggcgattcac ctggccgacc ctggctacgc ggcgctgatc
    49081 ctgccgcgct ccggtctcgg gcacaagcac ggcatcgtgc tgggcaacct ggtcggactg
    49141 atcgacagcg actaccaggg ccagctgatg gtcagcgcct ggaaccgcag cgccagcgcc
    49201 ttcaccatcg agccgatgga gcgcatcgcc cagctggtca tcgttccggt ggtgcaggcc
    49261 agctttcaga tcgtcgacgc cttcgaggca tcgcagcggg gtgcgggcgg ctacggctcg
    49321 accggtcgcg gttgatggcg ggcgtgttgg cgcgggtgtg accgctctgc cagaatgcca
    49381 gggtgtctgg tgccgcactt accgcgctgc gccagggcat gtttttccag gagtaccacc
    49441 atgcatcttt ctcaacgatt cgtccgttcg ctcgccaccg ccgccgcagc actgacgctg
    49501 gccgcctgcg cgcagcacgg gggctacccg cagggcaact acccaggcag ctatccgggc
    49561 aaccaggcct actcgcagtt cggccgcgtg acgaatgtcg aatacgtgcg cggcggccag
    49621 tcgcagggtg tggccggcgc agtgatcggc ggcgccgtgg gtggcctggc gggcagccag
    49681 gtgggtggcg gcagcggccg cacggcagcc acggtggcgg gcgtgatcgg cggcgcactg
    49741 atcggcaaca tgctggagcg caacatggac cgcaaccggg tcgagcatta ccgcgtcacg
    49801 gtgcagttcg acaacggcgg cgtgcgccag ttcgagtacg cccagcaccc caacgtgcag
    49861 atcggcgatc gcgtgcgggc cgaaggcgat cagctgtacc gctgaccggg tccgggcata
    49921 gacagcgcca cggcacctca gggtgccgtt gtcgcaggtt cagtgcaggt gcgggctgcc
    49981 cggcgccatc ggttgcagtc gaaagaagcc ggcgccggcg ctgccgcgct ccagctcatc
    50041 gtctaatgct tcgcgcaccg ccaccacctt cagttgcagc cgcaacgcca tgccggccag
    50101 cggatggttg gcatccagca ccacgtgctc ggggtagatc tcggtgatgg tgaacagcgc
    50161 gtccgccggc gcctgcgcca cgctgccggc cggcagcgaa gctgcctcga tcagcatgcc
    50221 ttcttcgatg ccgggtggca gctggtcgcg ccgggccaga aacaccagtt gctcatcaaa
    50281 gtcgccgaag gcgtcctccg gctccaggtt caggttcagc gtggcgcccg actcatgccc
    50341 ctgcagggcc gcgctgagga cgggcagcag gtcttcgctg ccgaccagaa actcgaccgg
    50401 ctcgtgcagt tcgtccaggg tgtctcccag gctgtctttc agcacccagg tcagggcgac
    50461 cacgcattgg ggggtgattt ccatggggcg attgtcgcac cgcaaaccgg gtcggccacg
    50521 gcttgcgtat gcttgcccaa tgcagatcga tcaacccctg accctgctgg gcgggctgtc
    50581 gccagcccgg ttcatgcagc ggcactggca gaaaaagccc ttgctggtgc gccaggccat
    50641 gcctggcttc aagcccttgc tgtcgcgcgc cgagctgttc gcgctggcgg gcgacgacgc
    50701 ggtggagtcg cgcctggtgc gccgttcatc ctccggctgg cagatgcgcc gtggaccgtt
    50761 ggcgcgccgc gcgctgccgc ccttgcagca ggccgactgg acgctgctgg tgcagggcgt
    50821 ggacctgcat caccagccgg cgcacgaact gctgcaggcc ttccgctttg tgcccgatgc
    50881 gcgcctggac gatctgatga tcagctacgc cagcgacggc ggcggcgtcg gcccgcactt
    50941 cgacagctac gacgtgttcc tgctgcaggc gcatggtcgg cgccgctggc gcatcggccg
    51001 acagaaggac ctgacgctgg tcgaaggctt gccggtcaaa atcctggccg atttccggcc
    51061 ccagcaggag ttcctgctcg aaccgggcga catgctctac ctgccgcccg gctgggcgca
    51121 cgatggggtg gcggtcggcg aatgcatgac ctactcgatc ggctttcgcc agccggcgcg
    51181 cgacgaaatg gtgcgcgagc tgctgcagcg cgtggccgac gacgccaccg acctggtcgg
    51241 ggaggcggtg taccgcgatc cggcccaacc ggccagcgcg cagccggcac agctgcccga
    51301 ggccatgctg gcgtttgccc gcgatgccct ccagtgtgcg cttgagcagc ccgaccacct
    51361 ggcgctgctg ctgggcgagt ggttgaccga gcccaagccc caggtctggt ttgaggccgg
    51421 cgacggtgcc gggcaggtgc aggccggcgt tcgactcgat cgccgcacac gcatgctcta
    51481 cgacgcgcgc catgtgttca tcaatggcga gggctttcgc gccagcggca ccgacgcgcg
    51541 gttgatgcgc gcgctggccg acgcgcggca actgaccgcg gcgcaggtgg gccggctcgg
    51601 cgccgacgcg cgcgcactgt tgcagcaatg gcatcgggcc gggtggttgc atggaaactg
    51661 aagccccgcc cagcccgctg ccttcgggca gcttcatcgg acacgagagt ttccgcaacc
    51721 atctgcgcac cggcctgctg gcggcggcgc aacagggctg gcgtgagctg ctgctgtcgg
    51781 acgccgattt tcggcactgg ccgctgggcg aacgcgaggt ggtcgaagca ctgacggcgt
    51841 gggtgcgtgg cgcacgccgc ttcaccatgc tggccaagag ctacgacgag gtgccgcgcc
    51901 tgcacgcacg cttcgttgag tggcgccgcc tgtggtccca caggatcgat tgccgggtgt
    51961 gccgcgaggc cgacgcgacc gagttgccca gcgccctgtg gacacccggc tggaccgtgc
    52021 agcggctgga tctgctgcac agcagcggtc tgtgcggcga cgatccggcg cgacgcctgc
    52081 tgctgcgcga gcagatcgac ggctggctgg tgcgcagcac gccggggttt cctgcctaca
    52141 ccctcggtct gtgacgtttg cacaccggcg tcgcgtccgc gcatcagggt ttgtcaccat
    52201 tgatcgacat gccccatata attaaggcct gagtggaagc ggtgttttgt gcttctccct
    52261 ccgtgcagca gggcgccgga acactggccg gtgtctttgc gccatccggc aattcttttc
    52321 ttgacctagg aaccttgaca tgaacaaatc cctcctgttg gccgccctga tcgccgccgc
    52381 ttcgctcgcc gcctgcggca agaaggaaga gcccgctccg gcgccggctc ctgcggccga
    52441 agcaccggct gctcctgccg ctgccgccgc cgcttcggcg gttgacgccg ccgccagcgc
    52501 ggttcagggc gccgccagcg atgccgctgc cgccgtgacc aacgccgctt cgacggcggc
    52561 caccgctgcc ggcgacgctg cttcggccgc cgccgccgct gcgaccgacg ccgctgccgc
    52621 tgccgccaac gccgccaaag atgcggccag cgccgccgcc aagtaacccc ttgcgggtcc
    52681 ggggtgtctg cgccgcaggc ctgatcggcc agttccagcg cccactcctg tgcaaatcag
    52741 ccaccttcgg gtggcttttt tgcgtctggg gcaggccatc tagactccga gccagcaggt
    52801 gccgtgccgc tcggccagca gcacctcgcc ggcggcgcag ccgagcacgg cggcgaaccg
    52861 ttggcgcacc agttcaggcc ggttgttgcg ttcgctcagg tgggccgcca ccacggtggt
    52921 cagctgcgca tggccgagcg cagtcagggc ggccgcggcc tgcgcgttgc tgagatggcc
    52981 gtgggtgccg cccacgcgcc gcttgaggaa gtcggggtag tcgccgcgcg ccagcagttc
    53041 gctgtcgtgg ttgctttcca gcagcagcgc gtgcacgccg gccagggcgt cgagcacctg
    53101 cggggtgaca tggcccagat cggtgagcac gccgagggag cgggcgccgt cggtgcagcg
    53161 cagttgcagc ggttcgcgcg cgtcgtgcgg cacggcaaag gggtggaact cgatgcctcc
    53221 gatggcaaac gtttctccgg cgcgtaccag ccgcgggcgc gcagcgcccg cgtcgggcgt
    53281 gtccagcgag cgggcggtgc cggcactggt ccacagcggg acaccatggc gccgcatcag
    53341 gctgcgcgcg cagcccaggt ggtcgccgtg ctcgtgggtg atgaagacgc catccagatc
    53401 ggtcagcgcc agcccttcgg cggccaggcg ggccgtcagc ggccgcaggc ccagcccgca
    53461 gtcgatcagc acccgggtgc ggcgcaggcc gtcgtcggcc tcgagcagcg tggcgttgcc
    53521 ggcgctgccg ctggccaggc tgcgaaagcg cagcatctcg gtgccgccgc tgcgccgtgt
    53581 ctacttcagt tcgtcggcca ggactttgac gatgcgctgg gcgtcggccc cgggggcggc
    53641 gccaccggcg cggtcgagca cgctgacctg gctgctctgg ccctcgctgc gcaccaccac
    53701 ctggtactgc tgcgtcggca gcggcgcgct gccgcggccg aacagcttgc cgaagaagcc
    53761 gggttcctgc ttttcgagcg acggatcgac gtagcgcacg aagtagatgc ctttgctgcg
    53821 gtcgcggtct tcgacggtga agccggtgcg gtccagcgcc aggccgacgc ggcgccaggc
    53881 gcggtcgaag tcttcggcca gttgcacctg gggcgtgccg ttggcggcgg tcaccaggcg
    53941 cgcctgcggc gtcgccgtcg gggctgcggc cgctgccagc gcggccttgg actgctcctg
    54001 cgagacgccc agacgcacca tcaggcggcg caggaattcg gcttccagct cggggtcgcg
    54061 cgcacgcggc tgccagatgg tgctgtcgcg ccgggccgag gaatacacct cttccatgcc
    54121 gcggtgggtg acgaagatgt cggtgccgcc ggcggggttg cgctccagcc ggatgcggaa
    54181 cttgtcgcgc tcgccggtgg agtagagcga atcgaagacc ttgccgatgg tctgccggat
    54241 gatgtcctgc gggatcttgg cgcggttttc ggcccagtcg gtttccatca ggcccatgct
    54301 gggctgatcg acggccagca ggaaaccgct ttccagccag aagtcgcgca cgtcgcccca
    54361 cagtttgtcg gccggccggt ccacgcgcag ccagcgctcg ccgtcctggc gcatgaagtg
    54421 cacgtcggca atgcggttgg ccgccgtggg cgtgcccgcc gccacggtgg ccgcctgccc
    54481 ggcctggtag gcgctggcgg tgacgctgcc gcccggggcg gcgtagcgcg actggccggg
    54541 cagttgcgac aggtcgggcg gcacttccag cgccacgccg cggccggcgc tcttgtagtc
    54601 gatccggtcg ggctccagca cggagcagcc gctggcggcc agcacggcca cgcccagcag
    54661 gggcaggcag gagcggggga tgaacgtcac gttcagcatt cctttgaaag aaattgggtt
    54721 ccggcgcccg ccgggcaagc gcaagcagct atgaatatta tagtaatcaa agcaggtcac
    54781 tgtcgcgcag ggcctgctcc accgcgggtt cacaggcctt ggacagccgc gtcatgggca
    54841 gcctcagcgc gccgccgcac aggcccaggc gggccatggc ccatttgacg gggatcgggt
    54901 tggcttcgac gaacaggtgc ttgtgcagcg gcatcagctt gaactggatt tccattgccc
    54961 ggcgacgatc gcccgcgatg gcggccacgc acagctcgtg catcaggcgc ggcgccacgt
    55021 tggcggtgac gctgatgttg ccctggccgc cgcacagcat cagtgccacg gcggtggcgt
    55081 cgtcgccgga gtagacggca aagcccttgg gcacgtcgcg aatcagccac tgcgcgcgct
    55141 cgatgttgcc ggtggcctcc ttgatgccga cgatgccggg cacctgcgcc aggcgcagca
    55201 cggtgtcgtg cagcatgtcg cccacggtgc ggccgggcac gttgtacagg accatgggca
    55261 gatcgcccac ggcttcggcg atggccttga agtgctggta ctggccttcc tgcgtgggct
    55321 tgttgtagta gggcaccacc tgcagctggc agtcggcgcc gacctttttg gcgtagcgcg
    55381 ccagctcgat cgcctcaagg gtggagttgg cgccgcagcc ggccatgatg ggcacgcgcc
    55441 cggcggcctg ttcgaccgag acgcggatga tctcctggtg ctcctccaca ttgacggtgg
    55501 gcgactcgcc ggtggtgccg accacgccga tgcagtcggt gccttcggcg atgtgccagt
    55561 cgatcagctt gcgcagggtg gggtagtcga cgctgccgtc ctcggtcatg ggggtgacga
    55621 gggcgacgat gctgccggtg atgggtgtca tgaggtccgg tttctctgga caggtaaatc
    55681 gggcatttta gtgcagtgtt tcgcgctgtc aggcactcat agaagccgga aaaaggcttg
    55741 cggcaaggcg cggctcgcag ccaaggcggc ggccttggca agaggcgcaa cgccgccgca
    55801 agcccttttt tcttcccgca gggcgtgccg ccaagaggcc atccgcgcgg cgcgtggtct
    55861 tgacatggct ccaccatgcc tgcggccacg cgccgcgcag ctggcccctt gtcgactcgc
    55921 tataagtacc tgacggcgcg aaacactgca cgggtgcgct gccagcgggt cggcgcgggc
    55981 ggcggcgtca gcgcagcggg tagcgcggcg gggcgtcggc agcggcgggg gcagcgccgg
    56041 tgcgcacggc cgcgatgcgc tgcacgaagc gatcgggcgc cggc
//
LOCUS       DAOQTZ010000006        54972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_661_length_54972_cov_14.722873, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000006 DAOQTZ010000000
VERSION     DAOQTZ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 54972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54972
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_661_length_54972_cov_14.722873"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PKN51_01540"
     CDS             <1..1029
                     /locus_tag="PKN51_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT83811.1"
                     /translation="ESIRIMGDKVSAKQAMIKAGVPCVPGSDGELPDDPAAIRRIAKS
                     IGYPVIIKAAGGGGGRGMRVVHTEAALLGAVQMTKGEAAAAFNNPAVYMEKFLQNPRH
                     IEIQVLADKHRNAVYLGERDCSMQRRHQKVIEEGPAPGIPRRLIERIGDRCVAACKKI
                     GYRGAGTFEFLYENGEFYFIEMNTRVQVEHPVTEWITGVDIVRTQIMVAAGEKLPFTQ
                     RQIQVKGHAIECRINAEDPFKFTPSPGRVTHWHMPGGPGVRVDSHVYTNYFVPPNYDS
                     MIGKIIVYGDTREQAIARMQTALAETVIEGIHSNVPLHRELMVDAKFMEGGTNIHYLE
                     KWLSEHQR"
     gene            1176..2063
                     /gene="prmA"
                     /locus_tag="PKN51_01545"
     CDS             1176..2063
                     /gene="prmA"
                     /locus_tag="PKN51_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNT83812.1"
                     /translation="MFELRLICPEDRVEPLSDALEALEALSVSVEDADADTAAERALF
                     GEPGLPPPAQSWSRSRVVALFGAEAPAREAARLLALQDFWDGGCQIAAIAAVPEQDWV
                     RLTQAQFEPVPITPSFWIVPTWHQVPAQAERVIRLDPGLAFGTGTHPTTRMCLRWLAA
                     HAPQGQRVLDYGCGSGILAIGAALHGAAAVDAVDIDPAAVQATQDNAAANGVVLRAGL
                     PELAQGAYDTVLANILATPLKVLAPLLAAHVAPGGALVLAGVLERQTEELQDAYRPWL
                     ALQVADREDGWVLLVARRA"
     gene            2106..>2837
                     /locus_tag="PKN51_01550"
     CDS             2106..>2837
                     /locus_tag="PKN51_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNT83813.1"
                     /translation="MSLITRCPACATTFKVVPDQLRIAAGWVRCGHCQQVFDAAAHML
                     PYSESVRLQVPPPPPPPAPAEPAPAAAVPPAPQAATPPAAPPAAAQAAAAPSFTPPGA
                     IFLRRPASPAAASGAPPDGWPSPHAETLPAPLSIEPPVALTSARASAPAAPEGDGATV
                     VGHEPDQPQGEIAPPAAPSDAQAATSAPEGVADAIVEADPPADSRADSEAETGAGAGA
                     DRGAAAVAPEPEPEAAPEPEPEAAPE"
     assembly_gap    2838..2937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2938..3679
                     /locus_tag="PKN51_01555"
     CDS             <2938..3679
                     /locus_tag="PKN51_01555"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HNT83814.1"
                     /translation="PEPEPEAAPEPEPEAAPEPEPEAAPEAEPEPEAEPEAEPEAAPE
                     AEAEAEAGASGGVVPEPEPASEPEPSAAPAAPSFVAEAKRRAFWSSRPMRALLWSAFF
                     LLALGLALQAALSRRDWLAARAPALAAPLGALCAVVGCEIRPHRQLEAIVIDGSAFQR
                     ASADGFRFSVSLRNTADLPVATPSLELSLTDVADQVLLRRVIAPAELGAPAALAARGE
                     FNASQRLTVADSASPSAIVGYRLVAFYP"
     gene            3738..4649
                     /locus_tag="PKN51_01560"
     CDS             3738..4649
                     /locus_tag="PKN51_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNT83815.1"
                     /translation="MATLICGSLAFDTIMSFEGRFAEQILPDQLHILNVSFLVPALRR
                     DYGGCAGNIAYAMRLLGGQPLPMATLGNDGGDYLERLRQLGIGTDFVRQIDDTYTAQA
                     MIMTDRDNNQITAFHPGAMMQAHRTAVSARADVRLGIVAPDGRDAMLQHAEQFHAAGI
                     PFVFDPGQGLPMFDGRELAHFVELASWVTVNDYEGRMLCERMGVEPAELSRRVRGLVV
                     TLGGAGCELWEDGRKTHVPAVQPEAVVDPTGCGDAWRGALLHGLEQGWPLARCAALGN
                     RLGALKIASRGPQNYQIDPVTLALAGG"
     gene            complement(4672..5517)
                     /gene="asd"
                     /locus_tag="PKN51_01565"
     CDS             complement(4672..5517)
                     /gene="asd"
                     /locus_tag="PKN51_01565"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229092.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNT83816.1"
                     /translation="MVTFAVLPQYLLPKRWLTRLAGRAAGARLGDLTHAAIRRFVARY
                     GVDMSEAADPRIESYASFNDFFTRALKPGARPLAAADYLCPVDGAISQFGAIERDQIF
                     QAKGHAYSTTALLGGDARLAAEFEHGQFATLYLSPRDYHRIHMPCAGRLRTMVHVPGD
                     LFSVNPVTARGVPGLFARNERVVCAFDTARGPLALVLVGATIVGSMATAWHGVVNPPR
                     PGRIRRWSYDEPSIELQRGEEMGRFLLGSTVVLLWPHGTLRFAPDWAPGGAVRMGQAL
                     GKALG"
     gene            5772..9233
                     /locus_tag="PKN51_01570"
     CDS             5772..9233
                     /locus_tag="PKN51_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HNT83817.1"
                     /translation="MRELTERRFLRAATRGRPLAGAIFAVTALLGTAGWAQAQSADLV
                     LAQQVVSPDPVPAGGVATITMTLQNNGPDAASGVKLGDTIPPGSTFVSLGASDGGTCT
                     SVAPYECTWAASVPFQASRTVTLQVILPAAGVWPNSASVSATTADPNGSNNSLSRNIT
                     AVAAADLRITASHNAAPGAVAGTPYSYTLNVSNGGPNALPSGQSATVTFNVPAGATIT
                     GVPTGSGWSCSPSGGYPLSNPPSPGALITCTRTDGLASGSSYPPITVPAMGNVNGTVS
                     ASFGVGSTYPDGDLANNTGLVDVPFTAGTDMALTKTASPTGTVAVGAPVTFTLQAQRR
                     GGVNPSNVVVTDTLPAGLTYVSHAAPAPWSCSFSAPTLTCTYAGTYTGAPYTNLPAIT
                     LTATVGLGPISNTATVALPTGQTDPVPGNNTGSVSVTGSNDADLSVNKTATISPVVPN
                     QDYNWTITVRNNGPVAVAPGQTVTVTENIPAGMRLNALPTGTGWTCSSSGGGAFPQNG
                     PVTVTCTRSDGRPASSNFPSITVPVRHTGTGTLNNNVCVNLTGAGPVDNNPGNNCVTR
                     GNASTATQADLSISKTATPEPVVVGQNVTYTLTVRNAGPDASTNVTLTDNLANLITAG
                     GLQSIVASQGSCTPATTPANGPTQNVSCNLGTLSSGASATVTIVARLANTTASDRTHT
                     NTASVTSPDVGDPNRSNNSASVNSTVQPRVDVTVSKSVTPNPVRVGEPLVYVITARNN
                     GPSTATNVTITDALPANTAMLGTATATNAGTCTAPADGAISGSVACSWASIPSGTQYT
                     ATFRVRPLAAALGTTIHNVVNIATDSTETDTNNNSATADATVIAADLDVLVHKTDDVD
                     PVVLGADTEYTITVTNVGPSYGTNLLITDTFPKAGTTPSARFSYQGSLSASIGGTPVA
                     APTCTEPAVGAISGTLTCSFPTIAIGASNAIVIKYRMRAESIVTAGDYGGTQRNNVVV
                     AVAETEREMDNNETDEDTTTRREAIATDLSLTKSINKQTLAAGEQAVFSLTVTNHGPL
                     DSLGAQVVDPLPAGMSFVSSVDGCVASGSTVSCAVGALASGASRTFTFTVQLDDPYNG
                     SSPITNTATLDAPGDTNPANNTGTARVRVPSREAVAPIPTLGEWAMVMLMATLAGLGA
                     TRLRRRT"
     gene            complement(9510..10727)
                     /locus_tag="PKN51_01575"
     CDS             complement(9510..10727)
                     /locus_tag="PKN51_01575"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HNT83818.1"
                     /translation="MAEPQHILVVGGGIGGLAAALALARLGLHVTLLEQAAEIGEIGA
                     GIQLGPNAFAALDALGVGENARRRAVFTDELIMMDAVDASVVGRFPVGEAFRARFGNP
                     YAVIHRADIHTSILEGVQGSDRIQFHTSTKVVHIEQDAASVTAIDQHGQRWQADALVA
                     ADGVRSVARDILFGDAPRVSGHVVYRAVVPVADMPRELRVNAPVVWAGPNCHLVHYPL
                     RGGSEYNLVVTFHSRQPEQWGVTDGSKQEVLSYFQGIHPRPRQLLECPKSWRRWATAD
                     RHPIPRWTAGRVTLLGDAAHGMLQYLAQGACMAIEDAVTLGLAMQACALDIGRAFALY
                     EQSRIPRTARVVLSAREMGRLYHADGVERLVRNQLWEGRTPERFYDALEWLYGWRADT
                     ALARPRAIEPRPS"
     gene            complement(10803..11501)
                     /locus_tag="PKN51_01580"
     CDS             complement(10803..11501)
                     /locus_tag="PKN51_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNT83819.1"
                     /translation="MTSYIFPPAPQAAVPVRGSPQQYAVSRIFCVGRNYAAHAREMGF
                     DPDREPPFYFNKTPIDLVPSGATVPYPLGSQNYHYEMELVIALGQPAFRVAPEQAQAC
                     VWGYACGLDMTRRDLQIKSREMGRPWDFGKDFEQSAVISELVPASAIGHPTRGAIQLS
                     VNGQLRQNADLKDLIWSVPEIVANLSHYYHLQPGDLIYTGTPEGVGAVQPGDHITGRI
                     DGVGEIALTIGQPE"
     gene            complement(11556..12629)
                     /locus_tag="PKN51_01585"
     CDS             complement(11556..12629)
                     /locus_tag="PKN51_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT83820.1"
                     /translation="MNATDALNPLGHAASLGRLEDLPPEYVRAIHDLSVAPLWPQLRG
                     LLPRHQPVRHTQPFMWRYQDVRPHLLRAGELTPIDKAERRVLVLCNPGHGLDKLVATP
                     SIYVGLQLILPGEVAPNHRHTPTAVRFVIEGEGGYTTVNGEKCPMHPGDLILTPALNW
                     HEHGHEGSGPVVWLDALDLPFIYQMEASYAIEGPPQAAGAGPDASQNRYLRAGLLPYA
                     SLGQALRPQYPQIRFPWQDVRQALGALASVTARDEAVQLAYVNPETGRPCLPTLGFSA
                     QQLRAGQDFSPRRRSASSVIHVIEGRGESEIDGVTLKWTQGDTLAVPTHAQVTHRATG
                     RDAYLFHVDDAPMQRALGFYEDF"
     gene            12807..14300
                     /locus_tag="PKN51_01590"
     CDS             12807..14300
                     /locus_tag="PKN51_01590"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNT83821.1"
                     /translation="MVSHAPFTPPVAFTDPAAALAQAQQIHDAGCAHLRAALAAYLAG
                     PAEPPAELARVRACYPFVRLRVPHGRHPDSRLAYGFVQRAGQYETTLTRPDLYADYYQ
                     EQFRLLIDNHGVTLEVGTSHQPIPLPFALAEDEHFEGQLSPAQRQRLPEVFDLPDLTA
                     MDDRIANGTHEVAPGAPEPLALFTAPRVDYSLHRLRHYTGTSPEHFQNFVLFTNYQFY
                     IDEFIALGEREMGRADSGYDSFVQPGNLVTRRAGLAPLPGDQRGAAPPRLPQMPAYHL
                     TRPDRGGISFVNIGVGPANAKTITDHIAVLRPHAWIMVGHCAGLRGSQQLGDYVLAHG
                     YVREDHVLDEDLPLWIPVPALAEVQIALQQAVADVTRLQGIELKRVLRTGTVATTDNR
                     NWELLGAGSPQRRFSQSRAIALDMESATIAANGFRFRVPYGTLLCVSDKPLHGEIKLP
                     GMANHFYRERVAQHLLIGIRAIERLRENGLHRLHSRKLRSFAEVAFQ"
     gene            complement(14339..16291)
                     /locus_tag="PKN51_01595"
     CDS             complement(14339..16291)
                     /locus_tag="PKN51_01595"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNT83822.1"
                     /translation="MHVRAFLDQSIRFSTLALCALGTLLALVWMALAGPGPWSTALML
                     LCAVVVGVGVYDLQQTRHAILRNYPILGHLRFALEFIRPEIRQYFIEGDVEAGEPFTR
                     AQRSVVYQRAKGVPDVRPFGTKLDVGANGYEWINHSMQTTHIESHDFRIWIGGQPGVP
                     REGVAPCTQPYNASVFNISAMSFGALSANAILALNLGARMDGFAHDTGEGGISRYHRQ
                     HGGDLIWEIGSGYFGCRNDDGSFSPERFARQAVDPQIKMIEIKISQGAKPGHGGMLPG
                     AKVTPEIAAARGIPVGQDCVSPSAHSAFSTPIELMHFIARLRQLSGGKPVGFKLCIGH
                     IWEWFAIVKAMLETDITPDYIVVDGAEGGTGAAPIEFADHMGAPVQEGLNLVSNTLIG
                     VNLRHRIKIGAAGKVINSFDLARMFALGADWCNSGRGFMMALGCIQAQVCHTGHCPTG
                     ITTQDPLRERALVVADKATRVAQYHANTLHALKELLQAAGLMHPDQLYTHHIVRRIDA
                     TRVRLLSAMMPTMRFGAILNDLEHQHNVYRLYWPLADAHSFSLHPPTAEELAASPGIR
                     PTIAGRPASAVQIAEELTRRRLPTPAPVSHLVPEQARTFHSSAVSPSQVPPEWTGSGS
                     DLLAAAMQLQDEHAHDAQAHADDRRP"
     gene            complement(16464..18623)
                     /locus_tag="PKN51_01600"
     CDS             complement(16464..18623)
                     /locus_tag="PKN51_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNT83823.1"
                     /translation="MELQTLRYAIEDTASGPVATITFDEQGSPVNTMCRQWQDDLVAV
                     TDQVLHDKARLRGLILASAKKTFFAGADLKGVMRTTQADATSVFTEIERIKKCFRSLE
                     TLGIPVVTCINGSALGGGMETALVGHHRIAVDDPKLQLGLPELTLGLIPGGTGITKFT
                     RLLGLMGAQPYIMESKLFGPREALEIGVVHELVASSDELRPRALAWIAEHPQAAHPWD
                     VKGYKMPGGTPANPKIAGALVVAPAMLKLKTRGRYPAPEAALAAMVEGAQVDYDTASR
                     IESRYLASLMTGPVARNMINTFFFNLNAIKSGQSRPQVATRFKPQKVGILGAGMMGAG
                     IAWAQASRGIATVLKDVSPDKAEQGKAYSAKLTQKRVDKGRMSAPEQQALLARITPTA
                     DAADLKGCELIIEAVFEQRELKAKVTQEAEPMLAEGGFFASNTSTLPISGLARASRDA
                     RKFVGIHFFSPVDKMKLVEIIRGRETDDETVARAYDYVLALGKLPIVVNDSRGFYTSR
                     TFGTFVMEGAAMLGEGIPAAAIENAAVQAGLPVGPLAVLDETALSLSVHVLDQTRADF
                     AAEGKTYQATPGELLVERMVKELGRPGRAGGGGFYDYPQGGRKHLWPQLKALFEKPDT
                     AWSVQDIQDRLLYRQSVETARCLAEGVLTSVHDANIGSIFGIGFPAWTGGAMQFIYGQ
                     GIEAFEQRCAALAARHGDGFRITDEVAATLRRLQPRY"
     gene            complement(18719..19924)
                     /locus_tag="PKN51_01605"
     CDS             complement(18719..19924)
                     /locus_tag="PKN51_01605"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNT83824.1"
                     /translation="MTEAYVFDAVRTPRGKGKKDGSLHEVKPIDLLAGTLEALRTRHD
                     FDTAAVDDVVMGIVSPIGEQGSVLPKVAALKAGWDWQCAGVQLNRFCASGLEAVNLAA
                     QKVRSGWEDLVVAGGVESMSRVPIGSDGGAWAQDPATNSATDFVPQGIGADLIATLEG
                     FTRQDVDAYAVESQQRAARARASGCFAGAVVPVKDFLGQTILSEDEFIKPRTTLEALA
                     GLKPSFEQLGAMGFDAVAIGRYPQVERIDHVHHAGNSSGIVDGAAAVLIGSEAAARTH
                     GLTPRARIVATAVSGADPTIMLTGPMPATRKVLAKAGLTIDQIDLFEVNEAFAAVPMK
                     FMKAFGVPHDKVNVNGGAIAMGHPLGATGAMILGTLIDELHRRGLRYGLATLCVGGGM
                     GIATIVERL"
     gene            complement(20066..22834)
                     /locus_tag="PKN51_01610"
     CDS             complement(20066..22834)
                     /locus_tag="PKN51_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HNT83825.1"
                     /translation="MLTDLQLAQIMRHASTRRRAQCLDPLNRAMQAHRIDANLQRVAA
                     FLAQLGHESGELQFLEELWGPTPAQKRYEPPGELARRLGNTQRGDGRRFKGRGPIQIT
                     GRANYARYGELLGLDLVGQPEQAADPEVGFEIAALFWDRNGLNALADAGDFETITRRI
                     NGGLNGLADRQRLHALALQVLQGGLPEPVGSSASTLPVAAAAARRRARTRAGSAALRL
                     ARGHEAMVADQAERPDALAATRTPRGRRPATGAVPTRRLDARPDTLDFRDQMFVPTLV
                     EVPSHVALGEYLEWQVPVLDQGTEGACTGYGLATVAHYLLRRRRVVADATPVSPRMLY
                     ELARRYDEWPGEDYDGSSARGAMKGWYKHGVCAETLYPSRPRGGRRTRPVPPAGGLDE
                     ARVSDALRRPLGAYFRVNHKDLVAMHSAIAEVGVLYASCTVHAGWSAVGADGVIGPST
                     RILGGHAFAIVAYDERGFWVQNSWGPGWGRNGFGLLSYDDWLEHGTDVWVARLGAPIT
                     LQRGASTASIHARSAAQSVGYSFAELRPHIVSVDNNGTLKAGGDYGSTPEELQRVFEQ
                     DIPRVSAGWAVPRVLLYAHGGLVSEQAGVQRLAEYRPLLLDAQVYPLAFIWRTDYWTT
                     ITNIIKDAVSRRRPEGLLDATKDFMLDRLDDALEPLARRLTGKSAWDEMKQNALAASA
                     RGGAAERVADLLLALRARLPGLEVHLVGHSAGSVLLAPLVDLLGARGLVVKTCTLWAP
                     ACRVELFTRHYQPALESRQIEKMALFALTDKVEQDDHCAGIYHKSLLYLISHAFEERP
                     RIPLFRDGEPFLGMERHIDRALMQRLGVDLVLAPNTEPADSLSASGAMHHGDFDDDAR
                     TVTATFRRVAPSVAAARPPASAPAGPRQAGADLLSHDLGALDPLFHRSAAALREQRQW
                     IDLRTRAA"
     gene            22912..24750
                     /locus_tag="PKN51_01615"
     CDS             22912..24750
                     /locus_tag="PKN51_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNT83826.1"
                     /translation="MIERAWRRDQRRRASTAVGVWGCGAFKGMGQITAHSVVVHRRCC
                     DRVGEGRPCQVPGGPPRRIRAGQRHEEEQASGPASGRHRVSEGDPLRIRCRRMPILED
                     GHYHRRSVPGSMAHAIGSRGMYKHCFRMACCAAALLTAGCASMAPPHERPIVAMPPTF
                     ASATPADVGTQTVAALGWRSYFTDPALTRLIEMALAHNPDLRTAVLRVQEARATYGVQ
                     RSAQLPTVGVGLDASRTRTPGDLSPSGSAVTANQFQASVGISSWEIDFWGRIASLNEA
                     ALQSYLATDAARRAASVSLIAQVADGWLGLREAQERLRLAQASAANQRESLRIFTRRL
                     EVGAASYLELTQVQTLLVQAELLVAQMQQQRDARRLSLNTLLGDLSDGSTVATEANTP
                     LDDTMIAPVAPGLPSDLLTRRPDVLAAEHRLQGANANIGAARAAFFPQISLTAFGGTA
                     STELERLFRGANRVWGFSPSLSLPIFDAGRKRANLALAEVRKNLAVVQYEQTVQNAFR
                     EVADALSAQHWALRQVEVHQRALAIERERARLSRLSYDHGASSFLEVLDAERNLISAE
                     QQLVQTRRALLSSRVALYAALGGGSHDVDETPPGPTSASPANFTRR"
     gene            24756..25829
                     /locus_tag="PKN51_01620"
     CDS             24756..25829
                     /locus_tag="PKN51_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNT83827.1"
                     /translation="MISSIKNKLLAVLAIAALAAGGWYAWQASTAKVDKPSFVAGNGR
                     IEATEVDIATKLAGRVNDILVTEGAFVKAGQVLATMQVQTLEAQLREAQAQRAQAVTS
                     VAAAEAQLAMRHSDKAALAAQVHQAAAERNAAQRRLARSETLVREGATSVQALDDDRT
                     RMESAVAAVAAAQAKVTSADASIGAAKAQVVSAQSQVRALDATLERIQAEIADSQLKA
                     PRDGRVQFVVAQPGEVLGAGGRVMNMLDVSDVYMTFFLPTEAAGRVALGADVRLVLDA
                     APDFVIPAKVSFIASAAQFTPKTVETASERQKLMFRVRARIDRQLLLEHLEHVKTGLP
                     GEAWVRLDPAQDWPTRLALKARP"
     gene            25826..28570
                     /gene="rbbA"
                     /locus_tag="PKN51_01625"
     CDS             25826..28570
                     /gene="rbbA"
                     /locus_tag="PKN51_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HNT83828.1"
                     /translation="MTGLANAPVVRVEALRLRHGKVQALAGVSLELPAGRMVGLIGPD
                     GVGKSSLLSLLAGARAVQEGRVEVLGGDMASKRHRDTVGPRIAYMPQGLGKNLYPTLS
                     VEENLQFFARLFGHDRAERRRRIDELTQATGLHPFLDRPAGKLSGGMKQKLGLCCALI
                     HDPDLLILDEPTTGVDPLARAQFWDLIANIRTGRPGMSVVVATAYMDEAQGFDWLVAM
                     DDGRVLATGTPAELLERTGSDALEAAFIALLPESRKRGHAPVVIPPLQADADDIAIEA
                     KDLTMRFGDFVAVDRVSFRIRRGEIFGFLGSNGCGKSTTMKMLTGLLPASAGQAWLFG
                     REVDPRDIATRNRVGYMSQAFSLYSELTVRQNLVLHARLFDVPEADIPARVAEMVERF
                     GLGPVLDELPESLPLGIRQRLSLAVAMVHKPELLILDEPTSGVDPVARDAFWRLLVEL
                     SRRDRVTIFISTHFMNEAERCDRMSMMHAGKVLDSAAPAKLVEKRGAATLEQAFIGYL
                     VEAGGATPASAAPIGAVAAAAPSREAAAHARRPFSLQRLYSYLWREALELQRDPVRAT
                     LALAGSIILMIVMGFGIGNDVAGIRYAVLDRDQSALSNSYLLDIGGSRYFQEQPPIAD
                     YDELDRRMRNGELTLAIEIPPGFGRDLLRGRQVAIGAWFDGAMPQLAETVRGYIQGMH
                     QHWLADQARGMQGGHTTGRISVENRFRYNPDVKSLPAMVPAVIPLLLMMLPAMLTALA
                     VVREKEMGSIINLYVTPVTRTEFLLGKQLPYVALAMVNFALMTLLAVTVFEVPIKGSV
                     LALVLASVLFCLISTGMGLLASAVTRSQIAAMFFAMVGTLIPTTQFGGLMDPVSSLEG
                     GARVIGEIYPASHMFIISRGVFNKALGFGDLSANFWPLLLAVPVIVGTAIALLRKQEV
                     "
     gene            28573..29700
                     /locus_tag="PKN51_01630"
     CDS             28573..29700
                     /locus_tag="PKN51_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT83829.1"
                     /translation="MWQRLSNIYHLGVKELWSLWRDPIMLVLTVFSFTFAVYSAASSM
                     PETLNNAPIAIVDEDQSQLSQRIVSAFYPPQFTAPFLIDQSRVDPGMDSGVYTFALII
                     PPNFQRDVLAGRDAQVQLNTDATRMSQAFNGSGFIQQVVLGEVQEFVQRYRAGAEPAV
                     GLELRARFNPALEKSWFGGLAQIINQITMLSIVLAGAALIREREHGTVEHLLVMPVTP
                     AEIMLAKIWSMGLVVLLASAVSLHVVVRWALGVPVAGSLPLFFFGAALLLFATTSMGI
                     FMATVARNMPQFGMLVMLVLLPLQMLSGGQSPRESQPGWLQDLMLAAPTTQFVKFGQA
                     ILFRGAGIEVVWVQLLAMALIGAALFAFSLARFRKTIGQMA"
     gene            complement(29842..31803)
                     /gene="htpG"
                     /locus_tag="PKN51_01635"
     CDS             complement(29842..31803)
                     /gene="htpG"
                     /locus_tag="PKN51_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNT83830.1"
                     /translation="MSTQTHAFQAEVANLLHLVTHSLYSNKEIFLRELISNASDACDK
                     LRFEALADSSLYGDQPELRVRVAFDRNARTLSISDTGVGMSEQETIEHLGTIAKSGTR
                     EFMSRLTGDQKQDAQLIGQFGVGFYSGFIVADRITVETRRAGLPPEQGVRWVSSGSGD
                     FSVERLTLPERGTRVILHLREDALEFADGWRLKELIGKYSDHIALPIQMEGEKWEDGK
                     EEGQPGQMVKTGDWETVNQATALWTRPRKDVTDEQYRDFYQQVAHDWQPPLAWSHNRV
                     EGSTEYTQLLYLPSHAPMDLWDRDKKPGVKLYVRRVFIMDDAEQLLPRYLRFVKGVVD
                     SADLPLNVSRELLQESRDVRSIREGNTRRVLTMLEELAKAPAEAPAEAPAEAAGGEPA
                     TDVQNPPPAGDRYATFWREFGAVLKEGLGEDHANRERIARLLRYASTASDTPAVSLAD
                     YKARMKDGQKAIYYIAADTLAAASHSPQLEIFRKKGIEVLLMTDRVDEWALSYLREFD
                     GTPLQSVAKGAVDLGELQNEAEKQAAEAAAEAVRPLLDKLKDVLKDSVEDVRVTTRLV
                     DSPACLVVKDSGISLQLARVLKQAGQPVPDTRPVLEINPEHALVKKLDGHARFDDLAQ
                     VLFDQALLAEGGLPDDPAGYVRRVNALLV"
     gene            complement(32096..33394)
                     /locus_tag="PKN51_01640"
     CDS             complement(32096..33394)
                     /locus_tag="PKN51_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT83831.1"
                     /translation="MTPTPPPGPDRPKQAARKRWRAADLRGAARLATQATMGVTRIVE
                     GVHRSVLGTLGLPGAAAPGRTRGITGMVYRSIQGVTLGVDAALQAALRRLEPWLERAQ
                     GAPDESAEAERDAVLAALNGVLGDRLAADDNPLALPMALRQHGRALELAALHAQGAAT
                     GRVLLLIHGLCMNDRQWLRSGHDHGAHLARTLGYTPVYLRYNTGLHISVNGRALAQHL
                     EALLASWPVPVQELGILAHSMGGLVARSAVWHATQTGQRWPAQLRHLIFLGTPHHGAP
                     LARAGPWVDLLLASTPYSRPFTRLGHLRSAGISDLRYGHVLDADWQGRDRLARSPDTR
                     TPVPLPDGVACHAVAATLAAQRSPLAERLVGDGLVPLRSALGAHDNPARCLAFAPADQ
                     YVVAQRGHLDLLSDAAVARQLVRWLTPPGERDPATATAER"
     gene            complement(33421..34167)
                     /locus_tag="PKN51_01645"
                     /pseudo
     CDS             complement(33421..34167)
                     /locus_tag="PKN51_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(34313..35020)
                     /locus_tag="PKN51_01650"
     CDS             complement(34313..35020)
                     /locus_tag="PKN51_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT83832.1"
                     /translation="MAHPSALHERRRPLNAAELAGVPWLSHLKAEEFDRAAPELRISL
                     AEPGELVCRRGRPVTYWFGVVDGLLKMSTETAEGQTITYSGLAPGGWFGEGTVIKREP
                     YRYNVSALRRSLVAGLPADTFHWLLDHSIGFNRFIMNQLNERLGHFIGTLEADRTPKP
                     EQRVARSIAALVNPVLFPGVGEVLRITQQELAYLVGLSRQRVNEALQSLSAQQLLRIE
                     YGGLRILDLAALRSRDF"
     gene            35164..37113
                     /locus_tag="PKN51_01655"
     CDS             35164..37113
                     /locus_tag="PKN51_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT83833.1"
                     /translation="MATTFPRLLQKHAAERPGDPALREKEYGIWQTWSWQRALDEVRW
                     LACGLTALGFTPGQNLAIVGDNRPHLYLMFLATQSLRGVPVPMYQDAVATEMAFVLRD
                     AEVHFALAEDQEQVDKLLELRAATGEQAVPGLTHIIYDDPRGMRNYRQPGLISVDELM
                     ALGRAHEREHPQLFADLVAQGQPDDVAVILYTSGTTGRPKGVCQTHAAFVASARGGVE
                     TDGLKAGDDVLSYLPPAWVGDFLFSMAQWLVAGFTINCPESSDTVGIDMREIGPTYYF
                     APPRVFEGMLTSVSIRMEDAARFKQRLHAHFMAVARRVGSDLLDGKPVGLADRLRYGI
                     GNLLVYGPLKNAMGLSRIRVAYTAGAAIGPDLFRFFRSIGVNLKQLYGQTETCAYVCI
                     QRNGQVKLESVGQAAPGIELKIADSGEVLVKGVSVLKEYYKRPDATAEVIDENGYFRT
                     GDAGVLDRDGHLRIIDRAKDVGKLSGGAMFAPNYIENKLKFFPQIKEAVCFGHEREQV
                     CAFINIDFEAVGNWAERQGLPYAGYVDLAGKPEVLAMMKDCVEQVNAGLAAEEGMQDT
                     QIARFLVLHKELDPDDDELTRTRKVRRGFIAEKYGVLIDALYSGRAEQFIETAVKFED
                     GRAGKVSATLKILDARTYPPVRSAA"
     gene            37155..37970
                     /locus_tag="PKN51_01660"
     CDS             37155..37970
                     /locus_tag="PKN51_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT83834.1"
                     /translation="MGAAAGSDLQAGGKKIGDVILDVQNISLRFGGVKALTDISFNVR
                     EHEIRSIIGPNGAGKSSMLNCINGVYTPSEGSITFRGKTFSHMNSRQVAEMGVARTFQ
                     NLALFKGMSVIDNIMTGRNLKIKSNLLMQALRIGPAEREEIAHREFVEHIIDFLEIQA
                     HRKTPVGQLPYGLQKRVDLGRALAMEPQVLLLDEPMAGMNVEEKQDMSRFILDVNDEF
                     GTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPDEVRGNEDVIRAYLGTSH"
     gene            37991..38920
                     /locus_tag="PKN51_01665"
     CDS             37991..38920
                     /locus_tag="PKN51_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT83835.1"
                     /translation="MGFFLETLFGGLMTGMLYSLVALGFVLIFKASGVFNFAQGAMVL
                     FAALAMARLAEWLPQWLGFENRVLANLLAFALAALIMWSVAWLIERLVLRHLVNQEGA
                     TLLMATLGITYFLDGLGQTLFGSASYQINIGMPKDPMFLFENVFQGGVLVNKEDLVAA
                     LIAAALVTGLTLFFQKTKTGRALRAVADDHQAAQSIGIPLSRIWVIVWTVAGVVALVA
                     GMIWGSKLGVQFSLATVALRALPVVILGGLTSVPGAIVGGLIIGVGEKLSEVYLGPFV
                     GGGIEIWFAYVLALAFLLFRPQGLFGEKIIDRV"
     gene            38989..40065
                     /locus_tag="PKN51_01670"
     CDS             38989..40065
                     /locus_tag="PKN51_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT83836.1"
                     /translation="MFYRENGQFKTSYRADQQIFPIAQDRWAVLLLVACAFVVVPLLA
                     SEYLFLSVLIPFLIMAMAAIGVNILVGYCGQISLGSGAFMAVGAYAAYNMYARIPGMQ
                     MLVAVLTGGAFAALVGIVFGIPSLRVRGLYLAVATLAAQFVCDWAFLRISWFTNHSTS
                     GSVSVSNLHVLGLPVETPVQKYLFCLSLLVVFGLLAKNLVRSAVGREWMAIRDMDVAA
                     AVIGIRPMYAKLSAFAVSSFIVGVAGALWGFVNLGSWEPAAFSVDRSFQLLFMVIIGG
                     LGSILGSILGAAFIIVLPIVLNLLLPWLGSLFGLEVSIAAVEHTQVMLFGALIVWFLI
                     VEPHGLAKLWSVGKQKLRIWPFPH"
     gene            40122..41444
                     /locus_tag="PKN51_01675"
     CDS             40122..41444
                     /locus_tag="PKN51_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT83837.1"
                     /translation="MKFRQLTLAAAAALTLGASASAMAQAQQFFPVLSGRTGPVAPNA
                     TPWANGHNDYMKLVNARGGINGVMTLVEECETAYATDKGVECYERLKGKHGGATVFQP
                     LSTGITFALTEKVPGDKISLITSGYGRSDTADGGVFKWNFPLIGHYWVAGDTVLQHIA
                     KKEGGWDKLKGKKFAIVYHDSPFGKELLPIVQERAKMHGFELQLLPVPAPGVEQKAIW
                     LQVRQQRPDFVIMQTWGVMTPTAIKEAVATGYPREKMFGTWWSGAEPDLKDVGAAAKG
                     YNAVMMQHGAEPQSAVVKEILEKVHAKGQGTGPKEEVGSVLYMRGVMGAMLAVEGVRA
                     AQERFGKGKVMTGEQARWGYENLNLTQAKLDALGFKGVMRPVSTSCQDHIGSGWARVH
                     TWDGSKFVWSSDWLEADQQIIKPMVKQSADKYAAEKKLTRRTAADCGS"
     gene            41569..42384
                     /locus_tag="PKN51_01680"
     CDS             41569..42384
                     /locus_tag="PKN51_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT83838.1"
                     /translation="MTTASAPIVLTVNGIEVIYNHVILVLKGVSLTVPESGIVAILGG
                     NGAGKTTTLRAVSNLLKGERGEVTKGSIELRGERIENLSPAELVKRGVVQVMEGRHCF
                     AHLTIEENLLTGAYTRSDKGEIAANLDKVYTYFPRLKTRRTSQAAYTSGGEQQMCAIG
                     RALMCNPSMVLLDEPSMGLAPQIVEEVFNIVRDLNTKERTTFLLAEQNTNMALKYADY
                     GYIMESGRIVMDGKASDLASNEDVKEFYLGVGGGERKSFKDVKSYKRRKRWLA"
     gene            42489..43739
                     /locus_tag="PKN51_01685"
     CDS             42489..43739
                     /locus_tag="PKN51_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT83839.1"
                     /translation="MTEYFDALETRDPAAREAALLAALPRQLAHAQQHSAAFADILAG
                     IDAAAVTSRAALARLPVTRKSELLARQQASRAAGRDPFGGFATGRWGSAMRRVYASPG
                     PIYEPEGCASDYWRTARALYAAGFRAGDLVHNAFSYHMTPGAFILEAGALAVGCTVFP
                     AGTGQTEQQLQAIAELRPQAYVGTPSFLRILFEKAAEGGADIRSLTKAMVSAEPFPPS
                     LRDWFAERGMTAYQSYATADVGLIAYETAARQGLVLDEGVIVEIVRPGTGDPVPEGEV
                     GEVVVTVLNPDYPLIRFGTGDLSAVLAGPCPTGRTNTRIKGWLGRADQTTKIRGMFVH
                     PGQVAEIVRRFPGVGRARLVVSGAMANDQMTLRVEAADLPGLAERIADAVRDVTKLRG
                     QIELVAPGSLPNDGKVIEDARSYE"
     gene            43862..44851
                     /locus_tag="PKN51_01690"
     CDS             43862..44851
                     /locus_tag="PKN51_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HNT83840.1"
                     /translation="MSPLFKLSIATVAALAALGAQAQAFPAAGKTVTLIVPFAAGGPT
                     DKVARDLAEALRKPLGGATVVIDNAAGAGSTIGAAKAFRAAPDGYTLLVTHIGMATTP
                     ALYRKLPYNVEEFEYLGLINEVPMVVVSKPGLPANNWAELKAWIGSNKGKVNLANAGL
                     GAASHLCGLMLQQALATEMTTIAYKGTAPAMTDLMGGQVDIMCDQTTNTTSQIEAKKI
                     KAFAVTTPKRITMPPVYKDLPTLEEVGMKGFNVSIWHGLYAPKGTPAAVVKVLNDALQ
                     AALKDADFIKKEEALGAVVAHDDRVTPAGHKKFVTAEVAKWGAVIKAAGQYAD"
     gene            44913..45290
                     /locus_tag="PKN51_01695"
     CDS             44913..45290
                     /locus_tag="PKN51_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83841.1"
                     /translation="MNRPLRLVDSITELRPGADTGCLAVSGSHGGISSAHYALAAQPM
                     LAVFNDAGVGKDVAGLAALPFLQRHGIAACTVAHTSARIGEARSTLHDGVVSHCNAQA
                     LSLGIEPGQACAALIRQLQGSGG"
     gene            45371..46309
                     /locus_tag="PKN51_01700"
     CDS             45371..46309
                     /locus_tag="PKN51_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT83842.1"
                     /translation="MAWAQGAWPSKPVRIVVPFAPGGTTDILARALAPELSKAFGQQF
                     IVDNRAGAGGNVGAEVVAKSPGDGYTLLMGTVGTHGINRALYPRLPYDPIKDFAPITL
                     VASVPNVMEMNADKAAQLGIHNVQDFIRYVKAHPGRLNMASSGNGTSIHLAGELFKHM
                     TGVFMTHIPYRGSGPALLDMVAGNMDVMFDNLPSSMAQIKAGKLKPLAVTSARRSAAL
                     PDVPTVEEAGGPALKGYEASSWFGLLAPAGTPADVVSRLQQESAKALASPAVKEKLLA
                     QGAVPGGIPPGEFALMIADEHAKWAKVVKASGAKVD"
     gene            complement(46370..46714)
                     /locus_tag="PKN51_01705"
     CDS             complement(46370..46714)
                     /locus_tag="PKN51_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HNT83843.1"
                     /translation="MLYKFKSQAAADVIMLKASAEELLKIIGKPASPTGIVTVDQAPA
                     AIAALKAEIERREAQGQGHAAPTSDDEARTQATADEFARQELVTLRQRAAPFIELLQI
                     SSAAGKDVVWGV"
     gene            46879..49209
                     /locus_tag="PKN51_01710"
     CDS             46879..49209
                     /locus_tag="PKN51_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HNT83844.1"
                     /translation="MKIVCIGGGPAGLYFALLMKRLNPAHDITVIERNRPYDTFGWGV
                     VFSDATMDNMRQWDAPTADAIQQAFNHWDDIELLFKGQVIRSGGHGFVGIGRKKLLNI
                     LQARCEALGVRLVFEQEVDDDAAFADADLIVASDGINSRIRTLHQAQFRPELVVRPNR
                     YIWLGTRKLYDAFTFIFEKTEHGWFQAHIYKFDQHTSTFIVECPEPVWRAHGLDQATQ
                     EASVAFCEKLFARHLQGERLMTNARHLRGSAWLNFTRVKCDRWWLRNANGAHVVLMGD
                     AVHTAHFAIGSGTKLALEDAIGLTREFKRLGDSPAQIEQVLSDYQASRSVEALRLQNA
                     AWNAMEWFEVCGERYCDQLEPPQFMYSMLTRSQRISHENLRLRDRQWLEGYERWFARR
                     DGLAVADAARPPPPMFTPYTVRGVTLKNRVVVSPMAQYSCVDGVPGDYHLVHLGARAL
                     GGAGLVMAEMTCVSPEGRITPGCPGLYNAEQKAAWQRIADWIHEHSDARFGMQIGHAG
                     PKASTRLAWDGTDLPLPQGNWPIVSASPQQYLPGISQRARALTQAEMQVILQQFVQAA
                     QSAADIGVDWLELHCAHGYLLSAFISPLTNQRTDEYGGSLENRLRFPLEVFRAMRAVW
                     PPQRPMSVRISATDWVEGGTTPADAVAIARAFKEAGADLIDCSSGQVSKQEQPVFGRM
                     WQTPFADRIRQEAGIATMAVGAISEADHVNSIIAAGRADLCAVARPHLANPAWTLTEA
                     ARIGYADMTWPRPYFQGKRQLEAGFERARAAGGAPI"
     gene            49220..49684
                     /locus_tag="PKN51_01715"
     CDS             49220..49684
                     /locus_tag="PKN51_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT83845.1"
                     /translation="MDLEARAHAEHPEALRLWLRLLTCTQMVERDLRNRLREAFDTTL
                     PRFDLMAQLERVPEGLRMNELSRRLMVTGGNVTGITDQLEAEGLVERVPVEGDRRAFH
                     VRLTPAGRQQFQAMAQAHEQWIVDAFAGLTSAEIRTLHQLLGRVKQHFLETT"
     gene            49681..50538
                     /locus_tag="PKN51_01720"
     CDS             49681..50538
                     /locus_tag="PKN51_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HNT83846.1"
                     /translation="MKHYIPSANPMRPQYRAQAAYQARHFAWQCDADGVATVTLDRPE
                     RKNPLTFDSYGELRDLFNGLRHATDVKVVVVTGAGGNFCSGGDVHDIIGPLTKMSMPE
                     LLEFTRMTGDLVKAMRGCPQPVIGAIDGVCAGAGAMMALACDLRYGTAATKTAFLFTR
                     VGLAGADMGACALLPRVIGQGRASELLFTGRAMSADEGLAWGFFNALHGSEELLAKAQ
                     AMAGQLAAGPMFGHAMTKNMLQQEWAMTIDQAIEAEAQAQAICMQTQDFRRAYEAFAA
                     RQKPVFGGD"
     gene            50538..51761
                     /locus_tag="PKN51_01725"
     CDS             50538..51761
                     /locus_tag="PKN51_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT83847.1"
                     /translation="MSTGILQPTALRPSTSHLGLPFFDEAHRRIAAELPDWTNAQGVD
                     ESDDRAACRDWVRRLGAGGWLRYCVPGDCGGALPQLDSRALVLLRETLAFHSPLADFA
                     FAMQGLGSGAISIGGSASQRQAYLPQVARGARIAAFALSEPEAGSDVAAMQLRAAGAR
                     PDCVAGQKDDSYLLDGRKTWISNGGIADFYCVFAKTDPAGGSRGISAFIVDAGTPGLD
                     DSAHIPVMAPHPLATLVFERCRVAGEQMLGELNGGFKLAMQTLDIFRASVAGAALGMA
                     RRALAEAIAHARQRRMFGQTLADFQLTQAKLGEMAALIDGAALLTYRAAWLRDTGQGS
                     PKERSVAAAMAKMTATENAQRVIDMALQMHGGLGVMVGRKVESLYRDIRALRIYEGAT
                     EVQQLIIGKSVLQEA"
     gene            51764..53386
                     /locus_tag="PKN51_01730"
     CDS             51764..53386
                     /locus_tag="PKN51_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT83848.1"
                     /translation="MSVPSAQTDRYVHDRLPPREQWPEFRYDLPELRIPDQANLVTVL
                     FDAARANGWSNRLLLCSPGQTLTYAEAEAQVARIAEMLIRDFKLEPGNRVLLRGGNSI
                     GMALAWLAVVKAGLIVVPTMPLLRALELGKVIDKAQPALALCEVDLLEDMVLAAQDAR
                     GRLPILAFGDPGRADGLEQRAARRSPDHAGCATAADDIALLAFTSGTTGVPKAVVTTH
                     RDILAACNTWPRHVLKMTPDDIVAGSPPLAFTFGTGGLLIYPMWAGASVYYPDVRFSP
                     ETMVRKMRDNGITVCYTAPTFYRQMAPYAKALGIPSLRICVSAGEALPDATRQLWKDA
                     TGIEMLDGIGGTEMWHIYISAAGADVRPGTIGKVVPGYQARVVDEHDQEVPRGTVGRL
                     AVIGPTGCRYMDDARQTRYVAHGWNHPGDACIQDEDGYFHYQARDDDMIVTAGYNVGG
                     PEVEDCLLTHPAVAECGVIGKPDAVRGMIIKAFVVLRPGQPDNEAMVEALQAHVRQHI
                     AAYKYPREIQFVTALPRTDTGKLQRFKLRELA"
     gene            53383..53781
                     /locus_tag="PKN51_01735"
     CDS             53383..53781
                     /locus_tag="PKN51_01735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNT83849.1"
                     /translation="MNPQRIHPEGWAPAKGYANGVAARGTQIYVGGQIGWNAQQQFES
                     DDFIAQVHQALRNVAAVLQAAGAGPEHMVRMTWYVLDRDEYNRRLKELGGVYRDVMGR
                     HFPAMSCVQVAGLMEARAKVEIEVTAVIPD"
     gene            53820..>54972
                     /locus_tag="PKN51_01740"
     CDS             53820..>54972
                     /locus_tag="PKN51_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNT83850.1"
                     /translation="MSKTKTPFSWQDPFALHDQLSADERAVQDAAHAYCQDKLLPRVQ
                     QAFRSEQTDPAIFREMGELGLLGPTIPEQYGGAGLGYVSYGLIAREVERVDSGYRSMM
                     SVQSSLVMVPINEFGNEATRQKYLPKLARGEWIGCFGLTEPNHGSDPGSMVTRAKKVP
                     GGYSLSGSKMWISNSPIADVFVVWAKDDEGAIRGFVLEKGWKGLSAPAIHGKVGLRAS
                     ITGEVVMDEVFCPEENAFPEVRGLKGPFTCLNSARFGIAWGALGAAEDCYARARQYVI
                     DRKQFGKPLAANQLIQKKLADMLTEISLGLQACLRLGRMKDEGHPPVELTSIIKRNSC
                     GKALDIARQARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDIHALILG"
BASE COUNT         8532 a        18825 c        18998 g         8517 t
ORIGIN      
        1 gagtcgatcc gcatcatggg cgacaaggtg tcggccaagc aggccatgat caaggccggc
       61 gtgccctgcg tacccggctc ggacggtgag ctgcccgacg acccggcggc catccgccgc
      121 atcgccaagt cgatcggcta cccggtgatc atcaaggccg ccggcggcgg tggcgggcgc
      181 ggcatgcgcg tggtgcacac cgaggcggcg ctgctgggcg cggtgcagat gaccaagggc
      241 gaggcggcgg ccgccttcaa caacccggcg gtctacatgg agaagttcct ccagaacccg
      301 cgccacatcg aaatccaggt gctggccgac aagcaccgca acgccgtcta cctgggcgag
      361 cgcgactgct ccatgcagcg gcgccaccag aaggtgatcg aggaaggccc ggcgccgggc
      421 atcccgcgcc gcctgatcga gcgcatcggc gatcgctgcg tggccgcctg caagaagatc
      481 ggctaccgcg gtgccggcac cttcgagttc ctgtacgaaa acggcgagtt ctatttcatc
      541 gaaatgaaca cccgcgtgca ggtcgagcac ccggtgaccg aatggatcac cggggtcgac
      601 atcgtgcgca cccagatcat ggtggcggcg ggcgagaagc tgcccttcac ccagcgccag
      661 atccaggtca agggccacgc catcgagtgc cgcatcaacg ccgaggaccc gttcaagttc
      721 acgccctcgc cggggcgcgt gacgcactgg cacatgccgg ggggccccgg tgtgcgggtc
      781 gactcgcacg tctacaccaa ctacttcgtg ccgcccaact acgactccat gatcggcaag
      841 atcatcgtct atggcgacac gcgcgagcag gccatcgccc gcatgcagac cgcgctggcc
      901 gaaaccgtga tcgagggcat tcacagcaac gtgcccttgc accgcgagct gatggtggac
      961 gccaagttca tggaaggcgg caccaacatc cattacctgg agaagtggct gagcgagcac
     1021 cagcgctgac cctgcgcggc catcgcgggc gctgggtggc cgtctggcgc cggggcacgg
     1081 cgcacgtcac cggctgcgaa ttccgaggct tttgtggctc tggcgcccga tgagaaagcg
     1141 ccggcagcta tggtttttga agtgttccac ctgccatgtt tgaacttcgc ctgatctgcc
     1201 cggaggaccg cgtcgaaccc ctgtccgacg cgctggaggc gctggaggcg ctgagcgtgt
     1261 cggtggaaga cgccgacgcc gacaccgcgg ccgaacgcgc cctgttcggc gagcccgggc
     1321 tgccgccgcc ggcgcagagc tggtcgcgct cgcgcgtggt ggccctgttc ggcgccgagg
     1381 caccggcgcg cgaggcagcg cgcctgctgg cgctgcagga cttctgggac ggcggctgcc
     1441 agatcgccgc catcgccgcc gtgcccgagc aggactgggt gcgcctgacg caggcgcagt
     1501 tcgagccggt gccgatcacg cccagcttct ggatcgtgcc gacctggcac caggtgccgg
     1561 cgcaggccga acgcgtgatc cggctcgacc ccgggctggc cttcggcacc ggcacccacc
     1621 ccaccacccg catgtgcctg cgctggctgg cggcccacgc gccgcagggg cagcgcgtgc
     1681 tcgactacgg ctgcggctcc ggcatcctgg ccatcggcgc cgcgctgcac ggcgcggccg
     1741 cggtcgacgc cgtggacatc gaccctgccg ccgtgcaggc cacgcaggac aacgccgccg
     1801 ccaacggcgt cgtgctgcgc gccggcctgc ccgagctggc ccagggcgcc tacgacaccg
     1861 tgctggccaa catcctggcc acgccgctga aggtgctggc gccgctgctg gccgcgcacg
     1921 tggcgcccgg cggtgccctg gtgctggccg gcgtgctgga gcgccaaacc gaggagctgc
     1981 aggacgccta ccggccctgg ctggcgctgc aggtggccga ccgcgaagac ggctgggtgc
     2041 tgctggtggc ccggcgcgcc tgagccagcg gccgcgcgcc gccggctccc tacaatccgc
     2101 gcaccatgag cctgatcacg cgctgcccgg cctgtgcgac caccttcaag gtcgtgcccg
     2161 accagctgcg catcgccgcc ggctgggtgc gctgtggcca ttgccagcag gtgttcgacg
     2221 ccgctgcgca catgctgccc tacagcgaaa gcgtgcgcct gcaggtgccg ccaccgccac
     2281 cgccaccggc gcccgccgag ccagcgccag ccgctgccgt gccgccggcg ccgcaggccg
     2341 cgaccccgcc cgcggcgccg ccggctgccg cgcaggccgc cgccgcgccc agcttcaccc
     2401 caccgggagc gatcttcctg cggcggccag ccagcccggc tgccgcgtcc ggtgcgccac
     2461 cggacggctg gccctcgccg catgccgaga ccctgccggc acccctgtcg atcgagccac
     2521 ccgtggcttt gacgagtgcc cgcgccagcg cgccggccgc gcccgagggc gacggtgcca
     2581 cggtggtcgg ccacgaaccc gaccagcccc agggcgagat cgcgccgccg gcggcgccgt
     2641 cagacgcgca ggccgcgact tcggcgcccg aaggcgtggc cgacgccata gtcgaagccg
     2701 atccgccggc cgattcgcga gccgattcag aggccgaaac cggtgcggga gccggggccg
     2761 atcgaggagc cgccgccgtt gcgcccgaac ccgaacccga agccgcgccc gaacccgaac
     2821 ccgaagccgc gcccgaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc
     2941 cgaacccgaa cccgaagccg cgcccgaacc cgaacccgaa gccgcgcccg aacccgaacc
     3001 cgaagccgcg cccgaagccg aacccgaacc cgaagccgaa cccgaagccg aacccgaagc
     3061 cgcgcccgaa gccgaagccg aagccgaagc cggggcgagc gggggagtcg tccctgaacc
     3121 cgagccggcg tcggagcctg aaccctccgc cgccccggcg gcaccgtcct tcgtcgccga
     3181 ggcgaagcgg cgcgccttct ggtcgtcccg gccgatgcgt gcgctgctgt ggtcggcgtt
     3241 cttcctgctg gcgctgggcc tggcgctgca ggcggcactg agccggcgcg actggctggc
     3301 cgcgcgtgcg ccggcgctgg cggcgcccct gggcgcgctg tgcgcggtgg tgggctgcga
     3361 gatccggccc catcggcaac tggaggccat cgtcatcgac ggctcggcct tccagcgcgc
     3421 cagcgccgac ggctttcgtt tcagcgtcag cctgcgcaac accgccgatc tgccggtggc
     3481 cacgccgtcg ctggagctga gcctgaccga cgttgccgac caggtgctgc tgcgccgcgt
     3541 gatcgcaccg gccgaactgg gcgcaccagc ggccctggcc gcgcgcggcg aattcaacgc
     3601 cagccagcgc ctgaccgtcg ccgacagcgc cagcccgtcg gccatcgtcg gctaccgact
     3661 ggtggccttc tacccctgaa ccccggggcg ccggcgcggc gcccgcatgg caacaccttc
     3721 taggagcttc accccccatg gccaccctga tctgcggctc gctggcgttc gacaccatca
     3781 tgagtttcga ggggcgcttt gccgagcaga tcctgccgga tcagctgcac atcctcaatg
     3841 tgtcctttct ggtgccggcg ctgcggcgcg actatggcgg ctgcgccggc aacatcgcct
     3901 acgccatgcg cctgctgggc ggccagccgt tgcccatggc cacgctgggc aacgacggtg
     3961 gcgactacct ggagcggctg cgccagctgg gcatcggcac cgacttcgtg cgccagatcg
     4021 acgacaccta caccgcgcag gccatgatca tgaccgaccg cgacaacaac cagatcaccg
     4081 cctttcaccc gggcgccatg atgcaggcgc accgcacggc cgtcagcgcg cgcgccgacg
     4141 ttcgtctggg catcgtcgcc cccgacgggc gcgacgccat gctgcagcac gccgagcagt
     4201 ttcacgccgc cggcattccc ttcgtgttcg accccggcca gggcctgccg atgttcgacg
     4261 gccgcgagct ggcgcacttc gtcgagctgg ccagctgggt caccgtcaac gactacgaag
     4321 gccgcatgct gtgcgaacgc atgggggtgg agccggccga gctgtcgcgg cgggtgcgcg
     4381 gcctggtggt gacgctgggc ggcgccggct gcgagctgtg ggaggacggc cgcaagaccc
     4441 atgtgccggc agtgcagccc gaggccgtgg tcgaccccac cggctgcggc gacgcctggc
     4501 gcggcgccct gctgcacggc ctggagcagg gctggccgct ggcgcgctgc gcggcgctgg
     4561 gcaaccgcct gggcgcgctg aagatcgcca gccgcgggcc gcagaactac cagatcgatc
     4621 cggtcacgct ggcgctggcc ggcggctgaa gggcggcgga tcgccgcgct gtcagcccag
     4681 cgccttgccc agggcctggc ccatgcgcac cgcgccgccg ggtgcccagt cgggggcaaa
     4741 gcgcagtgtg ccgtgcggcc acagcagcac cacggtggag ccgagcagaa agcgccccat
     4801 ttcctcgccg cgctgcaact cgatgcttgg ctcgtcgtag ctccagcgcc ggatccggcc
     4861 cggtcgcggc gggttgacca cgccgtgcca ggcggtggcc atgctgccga cgatggtggc
     4921 gcccaccagc accagcgcca gcgggccgcg cgcggtgtcg aacgcacaca ccacgcgctc
     4981 gttgcgtgca aacagccccg gcaccccacg cgccgtcacc gggttgaccg aaaacagatc
     5041 gcccggcaca tgcaccatgg tgcgcagccg cccggcgcag ggcatgtgga tgcggtggta
     5101 gtcgcgcggg ctcaggtaca gggtggcgaa ctggccgtgt tcgaactcgg ccgccagccg
     5161 cgcgtcgccg cccagcaggg cggtggtgct gtaggcatgg cccttggcct ggaagatctg
     5221 gtcgcgctcg atggcgccga actggctgat ggcgccgtcc accgggcaca ggtaatcggc
     5281 ggcggccagc gggcgcgcgc ccggcttgag cgcgcgggtg aagaagtcgt tgaagctggc
     5341 gtagctttcg atgcgcgggt cggccgcctc gctcatgtcg acaccgtagc gggcgacgaa
     5401 gcggcggatg gcggcgtggg tgaggtcgcc cagccgggcg ccggcggcgc ggccggccag
     5461 ccgggtcagc caccgcttgg gcagcagata ctggggcaga acggcgaacg tgaccatcgg
     5521 acgaaggcag aaaggcaggt ggtgggcgac gcgggggact gggattttat gggttgtcga
     5581 atggcaacgt ggcgatcggt tttcgtgacg cagttgcgaa aaccgcggat ggttgccggg
     5641 aagcggcaac ttcttgccta gactccgggc ggtttcaatg tggaacaaat gccgttcgct
     5701 gcgacccgcc cctcgtcacg ggcaaggttc ccttgtgcgg atggttcaaa tctgatgaat
     5761 aggaggttct catgcgtgag ttgacggaac gccgtttcct gcgcgcggcc actcgtggcc
     5821 gaccgctggc gggcgcgatt tttgcggtga ccgcgctgct gggcacggcc ggctgggcgc
     5881 aggcgcagtc ggccgatctg gtactggccc aacaggtcgt gagcccggat ccggtgccgg
     5941 cgggcggtgt ggcgaccatc accatgacgc tgcagaacaa cggtccggac gctgccagcg
     6001 gggtcaagct gggcgacacg attccgcctg gttccacgtt cgtcagcctt ggcgcctcgg
     6061 acggcggtac ctgcacctcg gtggcgcctt acgagtgcac ctgggccgcc agcgtcccgt
     6121 ttcaagcgtc ccgcacggtg acgctgcagg ttatcctgcc tgcggcgggt gtgtggccga
     6181 acagcgccag tgtgagcgcc accacggccg atccgaacgg cagcaacaac agcctgagtc
     6241 gcaacatcac cgccgtggcg gcggccgacc tgcgcatcac cgccagccat aacgcggcgc
     6301 ccggtgccgt cgcgggtact ccctacagct acaccttgaa tgtgtccaac ggcgggccca
     6361 atgcgctgcc gtcgggccag tcggccacgg tgacgttcaa cgtgcccgcc ggtgccacca
     6421 tcaccggggt acccacgggt tcgggctgga gctgctcgcc cagcggcggc tatccacttt
     6481 ccaacccgcc gagccccggc gcgctgatca cctgcacgcg cacagacgga ctggcttccg
     6541 gcagctccta cccccccatc accgtgcctg ccatgggcaa cgtcaatggc accgtgtcag
     6601 ccagcttcgg ggtcggatcg acctacccgg atggtgacct ggcgaacaac acggggctgg
     6661 tcgacgtgcc attcacggcg ggcaccgaca tggccctcac caagactgcc agcccgaccg
     6721 gcacggtggc cgtcggcgcc ccggtgacat tcacgctgca ggctcagcgc cggggcggcg
     6781 taaacccctc caatgtggtg gtcaccgaca ccctgccggc aggtctgacc tatgtcagcc
     6841 acgctgcgcc agcgccctgg agctgctcct tttctgcccc cacgctgacc tgcacctacg
     6901 ccggtaccta caccggcgcc ccgtacacca acctgcccgc catcacgttg acggccacgg
     6961 tcggtctggg tcccatcagc aacaccgcga cggtggcgct gccgacgggg cagaccgacc
     7021 cggtgcccgg caacaacacc ggttcggtgt cggtaaccgg cagcaatgac gccgatctgt
     7081 cggtgaacaa gacggccacc atttcgccgg tggttcccaa ccaggactac aactggacca
     7141 tcaccgtgcg caacaacggc ccggtggcgg tggctcccgg gcagaccgtc acggtgacgg
     7201 agaacattcc ggcgggcatg cggttgaatg ccctgcccac cggaaccggc tggacgtgca
     7261 gttccagcgg cggcggcgcc ttcccgcaga acgggccggt cacggttacc tgtacacgca
     7321 gcgacggccg acccgccagc agcaacttcc cgagcatcac ggtgccggtg cggcacacgg
     7381 gcacggggac gttgaacaac aacgtctgtg tcaatctgac cggtgctggt cctgtcgaca
     7441 acaaccccgg caacaactgc gtcacgcggg gcaacgccag cacggccact caggccgacc
     7501 tgtcgatctc caagacagcc acgccggaac cggtggtggt gggtcagaac gtgacctata
     7561 cgctcacggt ccgcaatgca gggcctgatg cctcgaccaa cgtgacgctc acggacaacc
     7621 ttgcgaacct gatcactgcc ggtggcctac agtccattgt ggcgtctcag ggctcctgca
     7681 cccctgccac cacaccggcc aatggtccga cccagaacgt gagttgcaac ctgggaacct
     7741 tgagcagtgg agcctcggcc acggtcacca tcgtggcccg gctggccaac accacggcga
     7801 gcgaccgcac gcacaccaac acggcgtcgg tcacctcccc tgacgtgggc gacccgaatc
     7861 gcagcaacaa cagtgccagc gtcaacagca cggtccagcc acgcgtggac gtgacggtca
     7921 gcaagtcggt cacgccgaac ccggtgcggg tcggggaacc actggtctat gtgattacgg
     7981 cacgcaacaa cggcccatcg accgccacca acgtgacgat cacggacgcg ctgccggcca
     8041 acacggcgat gctgggcacg gccacggcca ccaacgccgg cacctgcacg gcgccggccg
     8101 acggggccat cagcggcagc gtggcttgca gctgggcgtc gatcccgtcg ggcacccagt
     8161 acaccgccac cttccgggtg cggccgctgg ctgcggcgct gggcacgacg atccacaacg
     8221 tggtcaacat tgccacagac agcacggaaa ccgacaccaa caacaactcg gccactgccg
     8281 atgcgaccgt gattgcggcc gacctggacg ttctggttca caagaccgac gacgttgatc
     8341 ctgtggttct gggtgcggac accgaataca ccatcaccgt gaccaacgtt ggcccgtcct
     8401 atggcaccaa tctgctgatc accgacacct tcccgaaggc gggcaccacc cccagtgcac
     8461 gtttcagcta tcagggcagt ttgtcggcat cgatcggcgg cacaccggtg gcggcaccca
     8521 cctgcaccga accggcggtg ggcgccatat cgggcacgct gacctgcagt ttcccgacca
     8581 ttgcgatcgg tgcatccaac gccatcgtga tcaagtaccg catgcgtgcg gaaagcatcg
     8641 tcaccgcagg tgactatggc ggaacccagc gcaacaacgt ggtggtcgcg gtcgccgaga
     8701 ccgagcgcga gatggacaac aacgaaaccg atgaggacac caccacgcgc cgcgaggcga
     8761 ttgccaccga cctgtcgctg accaagtcga tcaacaagca gacgctcgct gcgggcgaac
     8821 aggcggtctt ctcgctgacg gtgaccaacc atggcccgct cgacagcctg ggcgcccagg
     8881 tggtcgatcc gctgccggcc ggcatgagct tcgtctcgtc ggtggacggc tgtgttgcca
     8941 gcggcagcac ggtgagctgc gcggtgggcg ccctggccag cggcgccagc cggacgttca
     9001 cctttacggt tcagctggac gatccgtaca acggttcgag ccccatcacc aacacggcga
     9061 ctctggacgc ccctggcgac accaacccag ccaacaacac cgggacagcc agagttcggg
     9121 tgcccagccg cgaagccgtg gccccgatcc cgacactggg tgagtgggcc atggtgatgc
     9181 tgatggccac cctggccggc ctgggtgcca cgcgtctgcg ccgccggact tgagaagctt
     9241 caacgaggca ctcatgcccg ccgtgaccgc cccaacacag ccgcgcgtcg ttgccagtgc
     9301 tcgccgtagc ccaggctgcg gctgtggctg gcacctcgat cggtggcgtt ggggtgctcc
     9361 tctcgggcgc ccggcatcgc ttcgcatctt ctgacgcgcc gggcgtgtcg actccaaccg
     9421 ccccctcgag gggcggtttt tttgtgtgtg tggggtgcgg gtgtgccgca cggggcgggt
     9481 tggatccggg ccctaccgaa tggggtgtgt cagctcgggc gcggctcgat tgcgcgcggc
     9541 cgcgccagcg cggtgtcggc gcgccagccg tacagccatt ccagcgcatc gtagaagcgc
     9601 tcgggcgtgc ggccttccca gagctggttg cgcaccaggc gctcgacgcc gtcggcgtgg
     9661 tacaggcgcc ccatttcgcg cgccgacagc accacgcgcg cggtgcgcgg gatgcggctt
     9721 tgctcgtaca gcgcaaaggc gcggccgatg tccagcgcgc aggcctgcat cgccaggccc
     9781 agcgtcaccg cgtcttcgat cgccatgcag gcgccctgcg ccaggtattg cagcatgccg
     9841 tgcgcggcgt cgcccagcag ggtgacgcgg cccgccgtcc agcgcgggat cgggtggcgg
     9901 tcggcggtgg cccagcggcg ccagctcttg gggcattcca gcaactggcg cgggcgcggg
     9961 tggatgccct ggaagtagct cagcacttcc tgcttgctgc cgtcggtgac gccccactgc
    10021 tccggctggc ggctgtggaa ggtgaccacc aggttgtatt cgctgccgcc gcgcagcggg
    10081 tagtgcacca ggtggcagtt ggggccggcc cagaccaccg gggcgttgac ccgcagctcg
    10141 cgcggcatgt cggccaccgg caccacggcg cggtagacca catggcccga cacgcgcggc
    10201 gcgtcgccga acaggatgtc acgcgccacc gagcgcacgc cgtcggcggc caccagcgcg
    10261 tcggcctgcc agcgctggcc gtgctggtcg atggcggtca cactggcggc gtcctgctcg
    10321 atatgcacca ccttggtcga ggtgtgaaac tggatgcggt ccgagccttg caccccctcc
    10381 aggatcgagg tgtggatgtc ggcgcggtgg atcaccgcat aggggttgcc aaagcgggcg
    10441 cggaaggcct cgccgaccgg aaagcgaccg accacactgg catcgacggc gtccatcatg
    10501 atcagctcgt cggtgaagac ggcgcggcgg cgcgcgttct cgcccacgcc cagcgcgtcc
    10561 agggcggcaa aggcgttggg gccgagctgg atgccagcgc cgatctcgcc gatctcggcc
    10621 gcctgttcca gcagcgtgac gtgcaggccc agccgggcca gtgccagcgc cgcggccagg
    10681 ccgccgatgc cgccacccac caccagtatg tgctgtggtt cagccatggg atttccgttg
    10741 actacaaaat taatagctgc ttgcgctttc caggcaagcc ctgaagcctt gtttttcttg
    10801 aatcactcgg gctggccgat ggtgagcgcg atctcgccca cgccgtcgat ccggccggtg
    10861 atgtggtcgc ccggctgcac cgcgcccacg ccttcgggcg tgccggtgta gatcagatcg
    10921 cccggttgca ggtggtagta gtgcgacagg ttggcgacga tctcgggcac gctccagatc
    10981 aggtccttca ggtcggcgtt ctgccgcagc tggccattga cgctgagctg gatggcgccg
    11041 cgggtcgggt gaccgatggc gctggcgggc accagctcgc tgatcaccgc cgactgctcg
    11101 aagtccttgc cgaagtccca gggccggccc atctcgcgcg acttgatctg caggtcgcgc
    11161 cgcgtcatgt ccagcccgca ggcgtagccc cacacgcagg cctgcgcctg ctcgggcgcc
    11221 acacggaagg ccggctggcc gagggcgatc accagctcca tctcgtagtg gtagttctgg
    11281 ctgcccagcg gatacggcac ggtggcaccg ctcggcacca gatcgatggg cgtcttgttg
    11341 aagtagaagg gcggctcgcg gtcggggtcg aaccccattt cgcgcgcgtg ggcggcgtag
    11401 ttgcggccga cgcagaagat gcggctgacg gcgtactgct gcgggctgcc gcgcaccggc
    11461 acggcggcct ggggggcggg ggggaagatg taggaagtca tgtggagtgc tcttgaaaat
    11521 gtagctgctg gcgctcgact ggcgggcgtt ggaggtcaaa aatcctcgta aaaaccgagc
    11581 gcgcgctgca tcggcgcgtc gtccacgtgg aacaggtagg cgtcccgtcc ggtggcgcgg
    11641 tgcgtgactt gggcatgcgt cggcaccgcc agcgtatcgc cttgcgtcca cttcagggtg
    11701 acgccgtcga tttcggattc gccgcggcct tcgatcacgt ggatcacgct gctggccgag
    11761 cgcctgcgcg gcgaaaaatc ctgcccggcg cgcagctgct gcgccgaaaa gcccagtgtc
    11821 ggcaggcagg gacggccggt ttcggggttg acgtaggcca gctgcaccgc ctcgtcgcgc
    11881 gcggtcacgc tggccagcgc gcccagcgcc tggcgcacgt cctgccacgg aaagcggatt
    11941 tgcgggtatt gcgggcgcag cgcctgaccg agcgaggcat agggcagcag gccggcgcgc
    12001 aggtacctgt tctgcgacgc gtccggtccg gcgcccgccg cctgcggcgg gccttcgatg
    12061 gcgtaggagg cctccatttg gtagatgaag ggcagatcca gcgcgtccag ccacaccacc
    12121 gggccgctgc cctcgtggcc atgctcgtgc cagttgaggg ccggcgtcag gatcaggtcg
    12181 cccgggtgca tggggcattt ttcgccgttc accgtggtgt agccgccttc gccttcgatg
    12241 acgaagcgca ccgccgtcgg cgtgtggcgg tggttgggcg ccacctcgcc cggcagaatg
    12301 agctgcaggc cgacgtagat ggagggcgtg gccaccagct tgtccagccc gtggcccggg
    12361 ttgcacagca ccagcacccg gcgctcggcc ttgtcgatcg gcgtcagctc gccggcgcgc
    12421 agcaggtgcg ggcgcacgtc ctggtagcgc cacatgaagg gctgcgtgtg gcgcaccggc
    12481 tggtggcgcg gcagcagccc gcgcagctgc ggccacaggg gcgccaccga caggtcgtgg
    12541 atggcccgca cgtactcggg cggcaggtct tccagccggc ccagcgacgc ggcgtggccg
    12601 agcggattca gggcgtcggt ggcgttcatg gggtgtctcc aggggtgagg ccgggcgccg
    12661 cgtgcggccc gggcgatgga tttaatatgt atgcttagta ttctaacggc atgcgttcgc
    12721 ccatgcaagg gggcgcggtg ggcgacgccg gtgcggcgcc gcccgcgatg acgcacggcg
    12781 gtcacacagg ccatggacaa tgcgccatgg tctcgcatgc tcccttcaca ccccccgttg
    12841 ccttcaccga cccggcggcc gccctggccc aggcccagca gatccacgac gccggctgcg
    12901 cccatctgcg tgcggcgctg gcggcctacc ttgccggccc ggccgaaccg ccggccgagc
    12961 tggcgcgcgt gcgtgcctgc tacccgttcg tgcggctgcg tgtgccgcac ggccggcatc
    13021 ccgactcgcg cctggcgtac ggattcgtgc agcgagccgg tcagtacgaa accacgctga
    13081 cgcgccccga tctgtacgcc gactactacc aggagcagtt ccgcctgctg atcgacaacc
    13141 acggcgtgac gctggaagtg ggcaccagcc accagccgat ccccttgccc ttcgcgctgg
    13201 ccgaggacga gcacttcgag ggccagctca gcccggcgca gcgccagcgc ctgcccgagg
    13261 tgttcgacct gcccgatctg accgccatgg acgaccgcat cgccaacggc acgcacgagg
    13321 tggcgcccgg cgcgcccgaa ccgctggcgc tgttcaccgc gccgcgcgtg gactactcgc
    13381 tgcaccgcct gcggcactac accggcacct cgcccgagca ctttcagaac ttcgtgctgt
    13441 tcaccaacta ccagttctac atcgacgaat tcatcgccct gggcgagcgc gaaatgggcc
    13501 gggcggacag cggctacgac agcttcgtgc agcccggcaa cctggtcacg cggcgtgccg
    13561 gcctggcgcc gctgccgggc gaccagcgcg gcgccgcgcc gccgcgcctg ccgcagatgc
    13621 cggcctacca tctgacgcgc cccgaccgcg ggggcatcag cttcgtcaac atcggcgtcg
    13681 gtccggccaa cgccaagacc atcaccgacc acatcgccgt gctgcgcccg cacgcctgga
    13741 tcatggtcgg ccactgcgcc ggcctgcgcg gcagccagca gctgggcgac tacgtgctgg
    13801 cgcacggcta cgtgcgcgag gaccatgtgc tcgacgaaga cctgccgctg tggatcccgg
    13861 tgccggcgct ggccgaggtg cagatcgcgc tgcagcaggc ggtggccgac gtcacgcggc
    13921 tgcagggcat cgagctcaag cgcgtgctgc gcaccggcac cgtggccacc accgacaacc
    13981 gcaactggga actgctgggc gctggcagcc cgcagcggcg cttttcgcag tcgcgcgcca
    14041 tcgcgctgga catggaaagc gccaccatcg ccgccaacgg ctttcgcttc cgcgtgccct
    14101 acggcaccct gctgtgcgtg agcgacaaac cgctgcacgg cgagatcaag ctgcccggca
    14161 tggccaacca cttctaccgc gagcgcgtgg cccagcacct gctgatcggc attcgcgcca
    14221 tcgaacgcct gcgcgaaaac ggcctgcacc gtctgcacag ccgcaagctg cgcagctttg
    14281 ccgaggtggc gttccagtga tccggcgccg ctgacccgcg gctttcagcg cgcgccgatc
    14341 acggccgccg gtcgtcggcg tgcgcctggg cgtcgtgcgc gtgctcgtcc tgcagctgca
    14401 tggcggcggc cagcaggtcg ctgccggagc cggtccattc cggcgggacc tgtgagggcg
    14461 agacggcgct gctgtggaag gtcctggcct gctccggcac caggtggctc accggcgcgg
    14521 gtgtgggcag ccgccggcgc gtcagctctt cggcgatctg taccgccgag gccgggcggc
    14581 cggcgatggt cggccggatg ccggggcttg ccgccagctc ttcggccgtg ggcggatgca
    14641 gcgagaagct gtgcgcgtcg gccagcggcc agtacagccg atagacgttg tgctggtgct
    14701 ccaggtcgtt caggatggca ccgaagcgca tggtcggcat catggccgac agcagccgca
    14761 cgcgggtggc gtcgatgcgg cgcacgatgt ggtgggtgta gagctggtcg gggtgcatca
    14821 ggccggccgc ctgcagcagc tccttgagcg cgtgcagggt gttggcgtgg tactgggcca
    14881 cgcgcgtggc cttgtcggcc accaccagcg cgcgctcgcg cagcgggtcc tgggtggtga
    14941 tgccggtcgg gcagtggccg gtgtggcaga cctgcgcctg gatgcagccc agcgccatca
    15001 tgaagccccg gcccgaattg caccagtcgg cgcccagcgc gaacatgcgc gccaggtcga
    15061 acgagttgat caccttgccg gcggcgccga tcttgatgcg atggcgcagg ttgacgccga
    15121 tcagcgtgtt gctgaccagg ttcaacccct cctgcaccgg cgcgcccatg tggtcggcaa
    15181 actcgatcgg cgccgcgccg gtgccgcctt cggcgccatc gaccacgatg tagtcgggcg
    15241 tgatgtcggt ctccagcatg gccttgacga tggcgaacca ttcccagatg tggccgatgc
    15301 acagcttgaa accgaccggc ttgccgcccg acagctggcg caggcgggcg atgaagtgca
    15361 tcagttcgat cggcgtcgag aaggcgctgt gcgccgacgg cgagacgcag tcctgaccga
    15421 ccgggatgcc gcgtgcggcg gcgatttcgg gcgtgacctt ggcgcccggc agcatgccgc
    15481 cgtgcccggg cttggcgccc tggctgatct tgatttcgat catcttgatc tgcggatcga
    15541 cggcctgtct ggcaaagcgc tcggggctga agctgccgtc gtcgttgcgg cagccgaagt
    15601 agccggagcc gatctcccag atcaggtcgc cgccgtgctg gcggtggtag cggctgatgc
    15661 cgccctcgcc ggtgtcgtgc gcgaagccat ccatcctggc gcccaggttg agcgccagga
    15721 tggcgttggc cgacagcgcg ccgaaactca tggccgagat gttgaatacg ctggcgttgt
    15781 acggctgcgt gcagggcgcc acgccttcgc gcggcacgcc gggctgaccg ccgatccaga
    15841 tgcggaagtc gtgcgattcg atatgggtgg tctgcatgga gtggttgatc cactcgtagc
    15901 cgttggcgcc gacgtccagc ttggtgccga aggggcgcac gtccggcacc cccttggcgc
    15961 gctgatacac cacgctgcgc tgcgcgcgcg tgaacggttc gccggcctcg acgtcgcctt
    16021 cgatgaagta ctgacggatt tccggccgga tgaattcgag cgcgaagcgc aggtgcccga
    16081 ggatcgggta gttgcgcaag atggcgtggc gcgtctgctg caggtcatag acacccacgc
    16141 ccaccaccac ggcgcacagc agcatcagtg ccgtcgacca tggaccgggg ccggccaagg
    16201 ccatccacac cagcgcgagc agggtgccca gcgcgcacag ggccagcgtg ctgaagcgga
    16261 tcgactggtc cagaaaggcc cgaacgtgca tggcagagct ccggtgttgg ccgagagcgc
    16321 tcctgccgat ccgccagccg ccacagcggg ctgagcacag ggcaggcgcc ctcggatcac
    16381 atcttagcca tgccccgccc ggtggaaaac cccgggcgcg ggcgctgcgc cggccgcgct
    16441 ggcaggggcg gccgggcgcc ggctcagtac cggggctgca ggcgccgcag cgtggccgcc
    16501 acctcgtcgg tgatgcggaa cccgtcgcca tggcgggccg ccagcgcggc gcagcgctgc
    16561 tcgaaggcct cgatgccctg accgtagatg aactgcatgg cgccgccggt ccaggcggga
    16621 aagccgatgc cgaagatcga gccgatgttg gcgtcgtgca cgctggtcag cacgccttcg
    16681 gccaggcagc gcgcggtctc gaccgactgg cgatagagca ggcggtcctg gatgtcctgc
    16741 acgctccagg ccgtgtcggg tttctcgaac agcgccttca gctggggcca taggtgcttc
    16801 ctgccgccct gcgggtagtc gtagaagccg ccaccgccgg cccggcccgg gcgccccagc
    16861 tccttgacca tgcgctcgac cagcagctcg ccgggcgtgg cctggtaggt cttgccttcg
    16921 gccgcgaagt cggcgcgggt ctgatccagc acatgcaccg acagcgacag cgcggtttcg
    16981 tccagcaccg ccagcggccc caccggcaga ccggcctgca cggcggcgtt ctcgatggcg
    17041 gcggcgggga tgccctcgcc cagcatggcc gcgccttcca tcacgaaggt gccgaaggtg
    17101 cggctggtgt agaagccccg cgagtcgttc accacgatgg gcagcttgcc cagcgccagc
    17161 acgtagtcgt aggcgcgcgc cacggtttcg tcgtcggtct cgcggccgcg gatgatttcc
    17221 accagcttca tcttgtccac cgggctgaag aagtggatgc cgacgaactt gcgcgcatcg
    17281 cggctggccc tggccaggcc gctgatcggc agggtgctgg tgttgctggc aaaaaagccg
    17341 ccctcggcca gcatgggttc ggcctcctgc gtgaccttgg ccttcagctc gcgttgctcg
    17401 aacacggctt cgatgatcag ctcgcagccc ttcaggtcgg ccgcgtcggc ggtgggcgtg
    17461 atgcgcgcca gcagcgcctg ctgctcgggc gcgctcatgc ggcccttgtc gacgcgcttt
    17521 tgcgtcagtt tggcgctgta ggccttgccc tgctcggctt tgtcgggcga cacgtctttc
    17581 agcacggtgg cgatgccgcg gctggcctgg gcccaggcga tgccggcgcc catcatgccg
    17641 gcacccagaa tgcccacctt ctgcggcttg aagcgcgtgg ccacctgcgg gcgcgactgg
    17701 ccgctcttga tggcgttcag gttgaagaaa aaggtgttga tcatattgcg cgccaccggg
    17761 ccggtcatca ggctggccag gtagcggctt tcgatgcgcg aggcggtgtc gtagtccacc
    17821 tgcgcgcctt cgaccatggc ggccagcgcg gcctcgggcg cggggtagcg cccacgcgtc
    17881 ttgagcttga gcatggctgg cgccaccacc agcgcaccgg cgatcttggg gttggcgggg
    17941 gtgccgccag gcatcttgta gcccttgacg tcccagggat gcgccgcctg cgggtgctcg
    18001 gcgatccagg ccagggcgcg cgggcgcagc tcatcgctgc tggccaccag ctcgtgcacc
    18061 acgccgatct ccagcgcctc gcgcgggccg aacagcttgc tctccatgat gtagggctgc
    18121 gcgcccatca ggcccagcag gcgggtgaat ttggtgatgc cggtgccgcc gggaatcagg
    18181 cccagcgtca gctcgggcag gcccagctgc agtttggggt cgtccaccgc gatgcggtgg
    18241 tggccgacca gcgccgtctc catgccgccg cccagtgcgc tgccgttgat gcaggtgacc
    18301 accgggatgc ccagggtctc cagtgagcgg aagcacttct tgatgcgttc gatctcggtg
    18361 aacacgctgg tcgcgtccgc ttgcgtggtg cgcatcacgc ccttgaggtc ggcgccggcg
    18421 aagaaggttt tcttggccga ggcgaggatc aggccccgca ggcgcgcctt gtcgtgcagc
    18481 acctggtcgg tcaccgctac caggtcgtcc tgccattgcc ggcacatggt gttgacgggc
    18541 gagccctgct cgtcaaaggt gatggtggcg acggggccgc tggccgtgtc ttcgatcgcg
    18601 tagcgcagtg tctggagttc catgatgact atgaaattga tagctgccgg cgcttgacca
    18661 tcaagcgccg gaggccgatg agattctgat gttcaggggc ggcaccgcag cgcgcggatc
    18721 acagccgctc gacgatggtg gcgatgccca tgccgccacc cacgcacagc gtggccaggc
    18781 cgtagcgcag gccgcgccgg tgcagctcgt cgatcagggt gcccaggatc atggcgccgg
    18841 tggcgcccag cgggtggccc atggcgatgg cgccgccgtt gacgttgacc ttgtcgtgcg
    18901 gcacgccgaa cgccttcatg aacttcatgg gcacggcggc aaaggcctcg ttcacttcga
    18961 acaggtcgat ctgatcgatc gtcaggcccg ccttggccag caccttgcgt gtggccggca
    19021 tcgggccggt cagcatgatg gtcgggtctg cgcccgagac ggcggtggcg acgatgcgtg
    19081 cgcgcggggt caggccatgg gtgcgcgccg ccgcttcact gccgatcagc accgctgccg
    19141 ccccatcgac gatgcccgaa gagttgcccg cgtggtgcac atggtcgatg cgctcgacct
    19201 ggggatagcg gccgatggcc acggcgtcga aacccatcgc ccccagctgc tcgaacgaag
    19261 gtttgaggcc ggccagcgcc tccagcgtgg tgcgcggctt gatgaattcg tcctcggaca
    19321 agatggtttg tcccagaaag tccttcaccg gcaccacggc gccggcaaag cagccgctcg
    19381 cgcgcgcccg cgctgcgcgc tgctgcgact cgacggcata ggcgtccacg tcctggcgtg
    19441 tgaagccttc gagtgtggca atcaggtcgg ccccgatgcc ttgcggcacg aagtcggtgg
    19501 cactgttggt ggccgggtcc tgtgcccagg cgccgccgtc ggagccgatg ggcacccggc
    19561 tcatgctttc cacgccgcca gccaccacca ggtcttccca gccgctcctg accttctgtg
    19621 ccgccaggtt gaccgcctcc aggcccgagg cgcagaagcg gttgagctgc acgcccgcgc
    19681 attgccagtc ccagccggcc ttcagcgccg ccaccttggg cagcaccgaa ccctgctcac
    19741 cgatcggcga gacgatgccc atcaccacgt cgtcgaccgc ggcggtgtcg aaatcgtggc
    19801 gcgtgcgcag cgcctccagc gtgccagcca gcagatcgat cggcttgacc tcgtgcaggc
    19861 tgccgtcctt cttgcccttg ccgcgcggcg tgcgcacggc gtcgaatacg taggcttcgg
    19921 tcatgggaag tctttcgtgc gtaagtgaag tggggggagc gtcagtatag atattttcca
    19981 aagcgatcgc ttgataaaaa tagatggggc attcccgcag cggctggcgc cgatccgaag
    20041 gtgggtacgg gccgttcgca cccgctcagg cggcgcgggt gcgcagatcg atccactggc
    20101 gctgctcgcg cagggccgcg gccgagcggt gaaacagcgg gtccagggcg cccagatcgt
    20161 gcgacagcag gtcggcgccc gcctggcgcg gccccgccgg ggcgctggcc ggcggccgcg
    20221 ccgccgccac gctgggtgcg acgcgccgga aggtggcggt gacggtgcgc gcgtcgtcgt
    20281 cgaagtcgcc gtggtgcatg gcacccgagg ccgacagcga atcggccggc tcggtgttgg
    20341 gggccagcac caggtccacg cccaggcgct gcatcagcgc gcggtcgatg tggcgctcca
    20401 tgccgaggaa cggttcgccg tcgcgaaaca gggggatccg cggccgctcc tcgaaggcgt
    20461 gggagatcag gtacagcagc gacttgtggt agatgccggc gcagtggtcg tcctgctcga
    20521 ccttgtcggt gagggcgaac agggccatct tttcgatctg gcgagactcc agcgcgggct
    20581 ggtagtgccg ggtgaacagc tcgacccggc aggccggcgc ccacagggtg caggttttca
    20641 ccaccaggcc gcgcgcgccc agcagatcga ccagcggcgc cagcagaacc gagccggcgc
    20701 tgtggccgac caggtgcact tccagcccgg gcaggcgggc acgcagcgcc agcagcagat
    20761 cggccacgcg ctcggccgcg ccgccccggg cgctggcggc cagggcgttc tgcttcatct
    20821 cgtcccaggc cgatttgccg gtgagccggc gcgccagcgg ctccagggca tcgtcgagcc
    20881 ggtcgagcat gaagtccttg gtggcgtcga gcaacccttc gggccggcgc cggctgaccg
    20941 cgtccttgat gatgttggtg atggtggtcc agtagtcggt gcgccagatg aaggccagcg
    21001 ggtacacctg ggcgtccagc agcagcgggc ggtattcggc cagccgctgc accccggcct
    21061 gctcgctcac caggccgcca tgggcataca gcagcacgcg cggcaccgcc cagccggcgc
    21121 tgacgcgcgg gatgtcctgc tcgaacacgc gctgcagctc ctcgggcgtg ctgccgtagt
    21181 caccgccggc cttgagcgtg ccgttgttgt cgacgctgac gatgtgaggt cgcagctcgg
    21241 cgaacgagta acccaccgac tgcgcggcac tgcgggcatg gatggaggcg gtcgacgcgc
    21301 cgcgctgcag cgtgatgggg gcacccagac gggccaccca gacgtcggtg ccgtgctcca
    21361 gccagtcgtc gtagctcagc aggccaaagc cgttgcggcc ccagcccggg ccccaggaat
    21421 tctggaccca gaagccgcgc tcgtcgtagg ccacgatggc aaaggcgtga ccgcccagga
    21481 tgcgtgtcga agggccgatc acgccgtcgg cgccgaccgc cgaccagccg gcgtgcaccg
    21541 tgcagctggc atagagcacg ccgacctcgg cgatcgccga gtgcatggcc accaggtcct
    21601 tgtggttgac gcggaagtag gcccccagcg ggcggcgcag cgcgtcgctg acgcgcgcct
    21661 cgtccaggcc gccggcgggc ggcaccgggc gggtgcgtcg gccgccgcgc gggcggctgg
    21721 ggtagagcgt ttcggcgcac acgccgtgct tgtaccagcc cttcatggcg ccgcgcgcgc
    21781 tggagccgtc gtagtcctcg cccggccatt cgtcgtagcg gcgtgccagt tcgtacagca
    21841 tgcgcgggct gaccggcgtg gcgtcggcca ccacacgccg gcgccgcagc aggtagtgcg
    21901 ccaccgtggc caggccgtag ccggtgcagg cgccttcggt gccctggtcg agcaccggca
    21961 cctgccattc gaggtactcg cccagcgcca cgtgggaggg cacctcgacc agcgtgggca
    22021 cgaacatctg gtcgcgaaag tccagcgtgt ccggtcgcgc gtccaggcgg cgcgtgggca
    22081 ccgcgccggt cgccgggcgg cggccgcggg gcgtgcgggt ggctgcgagg gcgtccggcc
    22141 gctcggcctg gtcggccacc atcgcttcgt gtccgcgcgc cagccgcagc gccgccgagc
    22201 cggcgcgggt gcgcgcgcgg cgccgggccg ctgcggcggc caccggcagc gtggaggcgc
    22261 tgctgccgac cggctcgggc agaccgcctt gcagcacctg cagggccagc gcgtgcaacc
    22321 gctggcggtc ggccaggccg ttcagcccgc cgttgatgcg ccgtgtgatg gtctcgaagt
    22381 cgcccgcgtc ggccagggca ttgaggccgt tgcggtccca gaacagggcg gcgatctcga
    22441 agccgacctc agggtcggcg gcctgctcgg gctggcccac cagatccagc ccgagcagct
    22501 cgccgtagcg tgcgtaattg gcgcgtccgg tgatctggat cggcccgcgc cccttgaagc
    22561 gccggccgtc gccgcgctgg gtgttgccga ggcggcgcgc cagctcgccg ggcggttcgt
    22621 agcgcttttg cgccggggtg gggccccaca gctcctccag gaactgcaac tcgcccgatt
    22681 catgccccaa ctgcgccagg aaggcggcga cccgctgcag gttggcgtcg atccggtgcg
    22741 cctgcatggc gcggttgagc ggatcgaggc attgcgcccg gcggcgcgtg gatgcgtggc
    22801 gcatgatctg cgcgagctgc aggtcggtca gcatggcggt ctcctcggtg cgcgccagcc
    22861 ggacttggtg ccgggattgc gccaacaagc cctccagcgc atcgtccgat catgatagag
    22921 cgcgcatggc ggcgtgacca gcggcgccgt gcgtcgactg ccgtcggcgt ctggggctgc
    22981 ggcgcgttca aaggcatggg ccagatcacg gcccattccg tggtggtgca ccgacgctgc
    23041 tgcgacaggg taggagaagg caggccatgc caggtacctg gcgggccacc caggcggatc
    23101 cgggcaggac aacgccatga ggaggaacag gcatcaggcc cagcgtccgg acggcatcgc
    23161 gtcagcgaag gcgatccact tcgcattcga tgccgacgga tgcccatctt ggaagacggg
    23221 cactaccatc gacgatctgt tcctggatcg atggcccatg cgataggctc cagaggtatg
    23281 tacaagcact gctttcgaat ggcctgctgt gcggcggccc tgctgactgc agggtgcgcc
    23341 tcgatggcgc caccgcatga gcgacccatc gtcgccatgc cccccacctt cgcgtctgca
    23401 acgcccgctg atgtgggcac gcaaaccgtc gccgccctcg gctggcgcag ctatttcacc
    23461 gatcccgcgc tcacgcgact gatcgagatg gccctggccc ataaccccga cctgcggact
    23521 gccgtgctgc gcgtgcagga agcgcgggcc acctacggcg tgcagcgcag cgcgcagctg
    23581 ccgacggtgg gtgtgggcct ggacgcgagc cgcacgcgca caccgggcga cctgagcccg
    23641 agtggcagtg cggtgacggc caaccagttc caggccagcg tgggcatcag cagttgggag
    23701 atcgatttct ggggccgcat cgccagtctc aacgaggcgg cgctgcagag ctatctggcg
    23761 accgatgcgg cgcgccgggc ggcaagcgtg tcgctgatcg cccaggtggc cgacggctgg
    23821 ctgggcttgc gtgaagcgca ggaacgactg cgcttggcac aggccagcgc ggccaaccag
    23881 cgcgagtcgc tgcgcatctt cacacggcgc ctggaggtgg gggcggcttc gtatctggag
    23941 ttgacccagg tgcagacgct gctggttcag gccgagttgc tggtggcgca aatgcagcag
    24001 cagcgcgacg cgcggcggct gtccctgaac acgttgctgg gcgatctgtc ggacggcagc
    24061 actgtggcga ccgaagccaa cacgccgctg gacgacacca tgatcgcacc tgtggcgcct
    24121 ggtctgccat cggacctgct gacccgccgg cccgacgtgc tggccgcgga gcatcggttg
    24181 cagggcgcca acgccaacat cggcgcggcg cgtgctgcgt tttttccgca gatcagcctg
    24241 acggctttcg gtggaacggc cagcaccgag ctggaacggc ttttccgggg cgccaaccgc
    24301 gtctggggct tttctcccag cctgtccctg cccatcttcg acgccgggcg caagcgcgcc
    24361 aacctggcat tggccgaggt gcgcaagaac ctggccgtgg tgcagtatga gcagaccgtg
    24421 cagaacgcct tccgcgaggt tgccgacgcc ctgtcggccc agcattgggc cttgcgccag
    24481 gtggaggtgc accagcgtgc cctggcgatc gaacgcgaac gtgcccgtct gtcgcgattg
    24541 agctacgacc acggcgcgtc cagctttctc gaggtgctcg acgccgagcg caacctgatc
    24601 tccgccgaac agcaactggt gcagacgcgc cgtgcgttgt tgtccagccg tgtggcgctt
    24661 tacgccgcgc tggggggcgg atcacacgac gtggacgaga cgccgcccgg tccgacgtct
    24721 gcctcccccg ccaacttcac ccgtcgctga tcgccatgat ctcctccatc aagaacaagt
    24781 tgcttgccgt cctcgccatc gctgcgctcg ctgcgggtgg atggtatgcg tggcaggcat
    24841 ccaccgccaa ggtggacaag ccgtcctttg tcgccggcaa cggtcgcatc gaagccaccg
    24901 aggtggacat cgccaccaag ctggcggggc gcgtgaacga catcctcgtc acggaagggg
    24961 ctttcgtgaa agccgggcag gtgctggcga ccatgcaggt gcagaccctg gaggcacagt
    25021 tgcgcgaggc gcaggcacag cgcgcacagg ccgtgacctc cgtggcagca gccgaggcgc
    25081 aactggccat gcggcacagc gacaaagccg cgctggccgc tcaggtgcat caggccgcgg
    25141 ccgaacgcaa tgccgcgcag cgccgcttgg cgcgctccga aaccctggtg cgggaggggg
    25201 ccacctcggt ccaggcgctg gacgacgatc gcacccgcat ggagagcgcc gttgccgccg
    25261 ttgccgccgc acaggcgaag gtgacttcgg ccgacgcgtc cataggcgcc gccaaagcgc
    25321 aggtggtgag cgcgcaatcg caggtcaggg cgctggacgc cacgctggag cgcatacagg
    25381 cggagatcgc tgacagtcag ctcaaggcac cgcgcgatgg ccgcgtccag ttcgtcgtgg
    25441 cgcagcctgg cgaggtgttg ggtgcgggcg gccgggtgat gaacatgctc gatgtctccg
    25501 acgtctacat gacattcttc ctgcccaccg aggcggcggg ccgggtcgcg cttggtgcgg
    25561 atgtgcgcct cgtgctggat gccgcgccgg acttcgtgat cccggccaag gtttccttca
    25621 tcgccagtgc ggcgcagttc acgcccaaga cggtggagac ggcgagcgag cgccagaagc
    25681 tgatgttccg ggtgcgcgcc cggatcgatc ggcagttgct gctcgagcac ctcgaacacg
    25741 tcaagaccgg cttgcccggc gaggcctggg ttcgcctcga tccggcgcag gactggccga
    25801 cccggctggc gttgaaggca agaccatgac agggctggcc aacgcacctg tggtgcgcgt
    25861 ggaagcgttg cgcctgcgcc atggcaaggt gcaggcgctg gcgggcgtga gcctggagtt
    25921 gccggccggc cgcatggtgg ggctgatcgg ccccgacggc gtgggcaagt ccagcctgct
    25981 gtcgctgctg gccggcgcgc gcgcggtgca ggaagggcgc gtcgaggtgc tgggcggcga
    26041 catggccagc aagcgccacc gcgacaccgt cggcccgcgc attgcctaca tgccacaggg
    26101 gctgggcaag aacctctacc ccacgctgtc ggtggaggaa aacctgcaat tcttcgcgcg
    26161 cctgttcggc catgacaggg ccgagcgccg ccggcgcatc gacgagctga cgcaagccac
    26221 cggcctgcac cccttcctcg atcgtccggc gggcaagctg tcgggcggca tgaagcagaa
    26281 gctggggctg tgctgcgcgc tgatccacga cccggatctg ctgatcctcg acgagccgac
    26341 caccggtgtc gatccgctgg cgcgcgcgca gttctgggac ctgatcgcga acatccgcac
    26401 cggtcgcccc ggcatgagcg tggtggtcgc cacggcctac atggacgagg cccagggctt
    26461 cgactggctg gtggcgatgg acgacggccg ggtgctggcc accggcacgc cggccgagct
    26521 gctcgaacgc accggcagcg atgcgctgga ggccgccttc attgcgctgc tgcccgaaag
    26581 caggaagcgc ggccacgcgc cggtggtgat cccgccgctg caggccgatg ccgacgacat
    26641 cgcgatcgaa gccaaggacc tcaccatgcg cttcggtgat ttcgtcgcgg tcgaccgcgt
    26701 gagctttcgc atccgccgcg gcgagatctt cggcttcctc ggctccaacg gctgcggcaa
    26761 gtcgaccacg atgaagatgc tgaccggcct cctgccggcc agcgcgggtc aggcctggct
    26821 gttcggccgc gaggtcgatc ccagggacat cgccacccgc aaccgcgtcg gctacatgtc
    26881 gcaggcgttc tcgctctaca gcgaactgac ggtgcggcag aacctggtgc tgcacgcgcg
    26941 gcttttcgac gtgccggagg cagatatccc ggcgcgcgtg gcggagatgg tcgagcgttt
    27001 tggcctcggg ccggtgctgg acgaactgcc cgaatcgttg ccgctgggca ttcgtcagcg
    27061 gctgtcgctg gcggtggcga tggtgcacaa gcccgagctg ctgatcctgg acgaaccgac
    27121 ctcgggcgtc gacccggtgg cgcgcgatgc cttctggcgc ttgctggtcg agctgtcgcg
    27181 gcgcgaccgg gtgaccatct tcatctccac ccacttcatg aacgaggccg agcgttgcga
    27241 ccgcatgtcg atgatgcacg cgggcaaggt gctcgacagt gccgccccgg ccaagctggt
    27301 ggaaaagcgc ggcgcggcga ccctggagca agccttcatc ggctacctgg tggaggccgg
    27361 cggcgcaacg ccggccagcg cggccccgat cggcgccgtg gcggctgctg cgccgtcgcg
    27421 ggaggcggca gcgcacgcgc gcagaccctt cagcctgcag cgcctgtaca gctacctgtg
    27481 gcgtgaagcg ctggagctgc agcgcgaccc ggtgcgcgcc acgctggcgc tggccggctc
    27541 gatcatcctg atgatcgtca tggggtttgg tattggcaac gacgttgccg gcatccgcta
    27601 cgctgtgctc gatcgcgacc agagcgcgct cagcaacagc tatctgctcg acatcggtgg
    27661 ttcgcgctac tttcaggagc agccgcccat cgccgactac gacgaactcg accggcgcat
    27721 gcgcaacggc gaactcacgc tggcgatcga gatcccaccc ggtttcggcc gtgacctgct
    27781 gcgcgggcgc caggttgcca tcggcgcctg gttcgacggc gcgatgccgc aactggccga
    27841 gaccgtgcgt ggctacatcc agggcatgca tcagcactgg ctggccgacc aggcgcgtgg
    27901 catgcagggc ggccacacga cgggccgcat ctcggtcgaa aaccgctttc gctacaaccc
    27961 cgacgtcaag agcctgccgg cgatggtgcc ggcggtgatc ccgctgctgc tgatgatgct
    28021 gcccgccatg ctgacggcgc tggcggtggt gcgcgagaag gagatgggat cgatcatcaa
    28081 cctgtacgtc actccggtca cccgcaccga gttcctgctc ggcaagcaac tgccctacgt
    28141 ggctctggcc atggtcaact ttgcgttgat gactctgctg gcggtgaccg tgttcgaggt
    28201 accgatcaag ggcagcgtgc tcgcgctggt gctggcctcg gtgttgttct gcctgatttc
    28261 caccggcatg ggcctgctgg cctcggcggt gaccaggagc cagatcgccg cgatgttctt
    28321 cgccatggtc ggaaccctga ttccgaccac gcaatttggc ggcttgatgg acccggtgtc
    28381 ctcactggag gggggtgcac gcgtcatcgg cgagatctac cccgccagcc acatgttcat
    28441 catcagccgc ggcgtgttca acaaggcgct tggctttggc gacctgtcgg ccaacttctg
    28501 gccgctgctg ctggcggtgc cggtgatcgt cggcaccgcg atcgcgctgc tccgcaagca
    28561 ggaggtctga gcatgtggca gaggctgtcc aacatctacc acctgggcgt caaggaactg
    28621 tggagcctgt ggcgcgaccc gatcatgctg gtgctgaccg tgttctcctt caccttcgcg
    28681 gtgtattcgg cggcgtcctc gatgcccgaa accctgaaca acgcgccgat cgccatcgtc
    28741 gacgaggacc agtcgcagct ttcgcagcgc atcgtcagcg cgttctaccc gccgcagttc
    28801 acggcgccgt tcctgatcga ccagtcgcgg gtcgacccgg gcatggactc gggcgtctac
    28861 accttcgccc tgatcattcc acccaatttc cagcgtgacg tgctggccgg gcgcgacgcg
    28921 caggtccagc tcaacaccga cgccacccgc atgagccagg ccttcaacgg cagtggcttc
    28981 atccagcagg tggtgctggg cgaggtgcag gagttcgtgc agcgctaccg cgccggcgcc
    29041 gagccggcgg tgggcctgga gttgcgcgca cgcttcaacc cggcgctgga gaagtcctgg
    29101 ttcggcggcc tggcgcagat catcaaccag atcaccatgc tgtcgatcgt cctggccggt
    29161 gcggcgctga ttcgcgagcg cgagcacggc accgtcgagc acctgctggt gatgccggtg
    29221 acgccggccg agatcatgct cgccaagatc tggtccatgg ggctggtggt gctgctggcg
    29281 tcggccgtgt cgctccacgt cgtggtgcgc tgggcgctgg gcgtgccggt ggccggctcg
    29341 ctgccgctgt tcttcttcgg cgccgcgctg ctgttgttcg ccaccacgtc gatgggcatc
    29401 ttcatggcca cggtcgcgcg caacatgccg cagttcggca tgctggtgat gttggtgctg
    29461 ctgccgctgc agatgctctc gggaggtcag tcgccgcgcg agagccaacc cggctggctg
    29521 caggacctga tgctcgctgc gccgaccacc cagttcgtca agttcggcca ggcgatcctg
    29581 tttcgcggcg ccggcatcga ggtggtgtgg gtgcagctgc tggccatggc actgattggc
    29641 gcggcgctgt tcgccttctc gctggcgcgc tttcgcaaga ccatcggcca gatggcgtga
    29701 tggccgctgc ctgcaggccg acgacaaacg tcttccatgc cgggcacccc gtgggtctga
    29761 ctgcggcggg tgggtgcccg cccgaaggag ccacgccgca tggcgcgacg agcacgctgc
    29821 gggcgcctgc cgtttctagg ctcagaccag cagcgcgttg acccgtcgca cgtagccggc
    29881 cgggtcgtcg ggcaggccgc cttcggccag cagcgcctgg tcgaacagga cctgagccag
    29941 atcgtcgaag cgcgcatgac cgtcgagctt cttcaccagc gcgtgctcgg ggttgatttc
    30001 gagcaccggg cgggtgtcgg gcaccggctg gccagcctgt ttgagcacac gcgccagctg
    30061 caggctgatg ccgctgtcct tgaccaccag gcaggccggc gagtcgacca ggcgggtggt
    30121 gacgcgcacg tcttccacgc tgtccttgag cacgtccttg agcttgtcca gcagcggcct
    30181 gacggcttcg gcggcggctt cggcggcctg cttttcggct tcgttctgca gctcgcccag
    30241 atccaccgca cccttggcca ccgactgcag cggcgtgccg tcgaattcgc gcaggtagga
    30301 cagcgcccac tcgtccacgc ggtcggtcat cagcaggact tcgatgccct tcttgcggaa
    30361 gatttcgagc tgcgggctgt ggctggccgc cgccagggtg tcggcggcga tgtagtagat
    30421 ggctttctgg ccgtccttca tgcgcgcctt gtagtcggcc agcgagacgg cgggcgtgtc
    30481 gctggcggtg gaggcgtagc gcagcaggcg ggcgatgcgc tcgcggttgg cgtggtcttc
    30541 gcccaggcct tccttgagca cggcgccgaa ttcgcgccag aaggtggcgt acctgtcgcc
    30601 ggcgggcggc gggttctgca cgtcggtggc cggctcgcca ccggccgctt cggcgggcgc
    30661 ttcggcgggc gcttcggcgg gcgccttggc cagctcttcc agcatggtca gcacgcggcg
    30721 cgtgttgcct tcgcggatcg accgcacgtc gcggctttcc tgcagcagct cgcggctgac
    30781 gttgagcggc aggtcggccg aatcgaccac gcccttgacg aagcgcaggt agcgcggcag
    30841 cagctgctcg gcgtcgtcca tgatgaacac gcgcctgacg tacagcttga cgccgggctt
    30901 cttgtcgcgg tcccacaggt ccatcggcgc gtggctgggc aggtacagca gctgggtgta
    30961 ttcggtgctg ccttcgacgc ggttgtggct ccaggccagc ggcggctgcc agtcgtgcgc
    31021 gacctgctgg tagaagtccc ggtattgctc gtcggtcacg tccttcctgg ggcgcgtcca
    31081 gagggcggtg gcctggttga cggtttccca gtccccggtc ttgaccatct ggccgggctg
    31141 gccctcttcc ttgccgtctt cccacttctc gccttccatc tggatcggca gggcgatgtg
    31201 gtcggagtac ttgccgatca gctccttcag gcgccagccg tcggcgaatt ccagcgcgtc
    31261 ttcgcgcagg tgcaggatca cgcgcgtgcc gcgctcgggc agggtgaggc gctcgacgct
    31321 gaagtcgccg ctgccgctgc tgacccagcg cacaccctgt tccggcggca ggccggcgcg
    31381 gcgcgtttcg accgtgatgc ggtcggcgac gatgaagccc gaatagaagc cgacgccgaa
    31441 ctggccgatc agctgcgcgt cctgcttctg gtcgccggtc aggcggctca tgaactcgcg
    31501 cgtgccgctc ttggcgatgg tgcccagatg ttcgatggtc tcctgctcgc tcatgccgac
    31561 gccggtgtcg ctgatgctca gcgtgcgggc gttcctgtcg aacgccacgc gcacgcgcag
    31621 ctcgggttgg tcgccgtaca ggctgctgtc ggccagcgcc tcgaagcgca gcttgtcgca
    31681 ggcgtcggag gcgttggaaa tcagctcgcg caggaagatt tccttgttgg agtacagcga
    31741 atgggtgacc aggtgcagca ggttggccac ttcggcctgg aaggcatggg tttgggtgct
    31801 catggtgcgg gatagccgtt catgggtgaa acaacgggag gtgcagcgcg cttcgttcgc
    31861 gtcggtcaat gcggcgcgcc gtgccgcaac tggggttgcg gggcggattt tcaagccgat
    31921 ggtgcgccac gggggcagaa tcgctattga aaaagtagct gctggcgctg gattgaagag
    31981 cgccgaatgc gattttgtct ccggatcggg ccgaggttcc gatgccggcc acgccgcctt
    32041 gcatcgcgtt cgcatccagg gcgcgccgcg tggcagaggc gatttcggcg acgcctcagc
    32101 gctcggcggt ggccgtggcc gggtcgcgct cgcccggcgg cgtcagccag cgcaccagct
    32161 ggcgcgccac cgccgcgtcg ctgagcaggt ccaggtggcc gcgctgggcg accacgtact
    32221 ggtcggcggg ggcaaaggcc aggcagcgtg cggggttgtc gtgcgcgccc agcgcgctgc
    32281 gcaggggcac cagcccgtcg ccgaccagcc gctcggccag cgggctgcgc tgcgccgcca
    32341 gcgtggcggc cacggcgtgg cacgccaccc cgtcgggcag cggcacgggg gtgcgggtgt
    32401 cggggctgcg ggccaaacga tcacggccct gccagtcggc gtcgagcaca tggccgtagc
    32461 gcaggtcgct gatgccggcg ctgcgcaggt gccccaggcg cgtgaaggga cggctgtacg
    32521 gggtgctggc cagcagcagg tcgacccacg ggccggcgcg cgccagcggt gcgccgtggt
    32581 gcggcgtgcc tagaaagatc aggtgcctga gctgcgccgg ccagcgttgg ccggtctgcg
    32641 tcgcgtgcca cacggcgctg cgcgccacca gcccgcccat gctgtgcgcc aggatgccga
    32701 gttcctgcac cggcaccggc cagctggcca gcagcgcctc cagatgctgg gccagcgcgc
    32761 gcccgttgac cgagatgtgc agcccggtat tgtagcgcag gtagacgggg gtgtagccca
    32821 gcgttcgcgc caggtgtgcg ccatggtcat ggcccgaacg cagccattgg cggtcgttca
    32881 tgcacaggcc atggatcagc agcagcaccc ggcccgtggc cgcgccttgc gcgtgcaacg
    32941 ccgccagctc gagtgcgcgc ccgtgctggc gcagggccat cggcaaggcc agcgggttgt
    33001 cgtcggccgc caggcggtcg cccagcacgc cgttcagcgc cgccagcacg gcatcgcgct
    33061 cggcctcggc cgactcgtcg ggcgcgccct gcgcccgctc cagccagggt tcgagccgcc
    33121 gcagcgcggc ctgcagcgcg gcgtccaccc ccagcgtcac gccctggatg ctgcggtaga
    33181 ccatgccggt gatgccgcgc gtgcggcccg gtgcggccgc gccgggcagg ccgagtgtgc
    33241 ccagcaccga ccggtgcacg ccttcgacga tgcgcgtcac gcccatcgtg gcctgggttg
    33301 ccaggcgcgc cgcaccgcgc aggtcggccg cgcgccaccg ctttcgcgcg gcctgctttg
    33361 gcctgtcggg tccgggcgga ggggtcgggg tcatggtggc aagggctggc tgcggtgggg
    33421 tcagaagccc tcgtgcgcgc ccaggtaccg ccactgaccg accggcagct cgcccaggcg
    33481 cacctggccg atgcgtacgc gcttgagtcc ggtgaccttc aggccgacca gctcgcacat
    33541 gcggcggatc tggcgcttct tgccctcgcg cagcacgaag cgcagctgct cggggttttg
    33601 ccagtcgacc tgggcgggct tgagcggctg gccgtccagg ctcaggccat ggcgcaggcg
    33661 cgccagctgt gcggccggaa aggcggcctg cacgttctcg gtctgcgcgc cgtagctgac
    33721 gcgcaccagg tattccttgt cgatcgtcga gtcttcgccg atcaactggc gcgccacgcg
    33781 gccgtcctgg gtcagcacca gcagaccgac cgagtcgatg tccagccgcc cggcgggggc
    33841 cagaccgcgc agctgggccg gcgcaaagcg ggtgcggctg gcgtcgccgc gccaatggct
    33901 gcgcgcgtgg atcagggtca cggcgggctg gtggccgtct tcggcctggc cgctgacgta
    33961 gccgaccggc ttgtgcagca ggatggtcac gcgttcggcc tgggcggcgt cggcgcgccg
    34021 gtccacacgc acctggtcgg ccgtggtcac cttcaggccc atcaccgcca cctggccgtt
    34081 gacgcgcacc cagccgcgct cgatccagtc gtcggcttcg cggcgcgaac acagccccag
    34141 atcggccagg cgcttgttga ggcggatcga cgccggcgcc gcgccggcgg cggcggtgtc
    34201 ggggcggggc gcacggcgaa agcgggcggg gtcggaggtc atgggcggtg ttgaatacta
    34261 cacaaataat agctgctggc gcttgttggt aaagcgatag aggccgattt gatcaaaaat
    34321 cccttgaccg cagcgcggcc agatcgagga tgcgcagccc gccatattcg atgcgcagca
    34381 gctgctgggc cgacagcgat tgcagcgctt cgttgacgcg ctggcgcgac aggcccacca
    34441 ggtaggccag ctcctgctgc gtgatgcgca gcacttcgcc cacgccggga aacagcaccg
    34501 gattgaccag cgccgcgatg ctgcgcgcca cgcgctgctc gggtttgggc gtgcggtcgg
    34561 cttccagtgt gccgatgaag tgtcccaacc gctcgttgag ctgattcatg atgaagcgat
    34621 tgaagccgat cgagtggtcg agcagccagt ggaaggtgtc ggccggcaga ccagccacca
    34681 ggctgcgccg cagcgcactg acgttgtagc ggtagggctc gcgcttgatc accgtgcctt
    34741 cgccgaacca gccgccaggc gcgaggcccg agtaggtgat ggtctgtcct tcggcggttt
    34801 cggtgctcat cttcagcagc ccgtcgacga cgccgaacca gtaggtcacc gggcggcccc
    34861 tgcggcacac cagctcgccc ggctcggcca gcgagatgcg cagctcgggc gcggcgcggt
    34921 cgaactcctc ggccttgagg tgcgacagcc agggcacacc cgccagctcg gccgcgttca
    34981 gcgggcggcg gcgttcatgc agggcggacg gatgggccat gtgcgggaaa tcccgggtcg
    35041 gatttgcccg caattgtcgt tcaaacgaca acccagcgtc aaagtggcgc cgtaccatgc
    35101 gcgtcgatcg aacgcatgcg cctggggcca cgccaggcgc tcgaatcaca ggagacgcgc
    35161 cccatggcga ccacctttcc ccgcctgctg cagaagcacg ccgccgagcg accgggcgat
    35221 ccggcgctgc gcgagaagga atacggcatc tggcaaacct ggagctggca gcgcgcgctc
    35281 gacgaagtgc gctggctggc ctgcggcctg acggcgctgg gcttcacgcc gggacagaac
    35341 ctggccatcg tgggcgacaa ccggccgcac ctgtacctga tgttcctggc cacgcagtcg
    35401 ctgcgcggcg tgccggtgcc gatgtaccag gacgcggtgg ccaccgaaat ggccttcgtg
    35461 ctgcgcgacg ccgaagtgca tttcgccctg gccgaggacc aggagcaggt cgacaagctg
    35521 ctggagctgc gcgccgccac cggcgagcag gccgtgccgg gcctgaccca catcatctac
    35581 gacgacccgc gcggcatgcg caactaccgc cagccgggcc tgatcagcgt cgacgagctg
    35641 atggcgctgg gtcgggcgca cgaacgggag catccgcagc tgttcgccga cctggtggcc
    35701 cagggccagc ccgacgacgt ggcggtgatc ctctacacct cgggcaccac cgggcgaccc
    35761 aagggggtgt gccagaccca cgcggcgttt gtcgcctcgg cgcgcggcgg ggtcgaaacc
    35821 gacgggctga aggccggcga cgacgtgctg tcgtatctgc cgccggcctg ggtgggtgac
    35881 ttcctgtttt cgatggcgca gtggctggtg gcgggtttca ccatcaactg cccggaatcg
    35941 agcgatacgg tcggcatcga catgcgcgag atcgggccga cgtattactt cgcgccgccg
    36001 cgcgtgttcg agggcatgct gacttcggtg tcgatccgca tggaagatgc ggcgcgcttc
    36061 aagcagcgcc tgcacgccca tttcatggcc gtggcgcggc gcgtcggctc cgacctgctg
    36121 gacggcaagc cggtgggcct ggccgaccgg ctgcgctacg gcatcggcaa tctgctggtc
    36181 tacggcccgc tgaagaacgc catgggcctg tcgcgcatcc gcgtcgccta caccgcgggc
    36241 gcggccatcg ggccggacct gttccgcttc tttcgctcca tcggcgtcaa cctcaagcag
    36301 ctctacggcc agaccgaaac ctgcgcctac gtctgcatcc agcgcaacgg tcaggtcaag
    36361 ctcgaaagcg tcgggcaggc cgcgcccggc atcgaactga agatcgccga cagcggcgaa
    36421 gtgctggtca agggcgtttc ggtgctcaag gaatactaca agcgccccga cgccaccgcc
    36481 gaggtgatcg acgagaacgg ctacttcaga accggcgacg ccggcgtgct ggaccgcgac
    36541 ggccacctgc gcatcatcga ccgtgccaag gacgtgggca aactgtctgg cggcgccatg
    36601 ttcgcgccca actacatcga gaacaagctc aagttcttcc cgcagatcaa ggaggccgtg
    36661 tgcttcggcc acgagcgcga gcaggtctgc gccttcatca acatcgactt cgaggccgtg
    36721 ggcaactggg ccgagcgcca gggcctgccg tatgccggct acgtcgatct ggccggcaag
    36781 cccgaggtgc tggccatgat gaaggactgc gtcgagcagg tcaacgccgg tctggccgcc
    36841 gaagagggca tgcaggacac gcagatcgcc cgcttcctgg tgctgcacaa ggaactggac
    36901 cccgacgacg acgagctgac gcgcacgcgc aaggtgcggc gcggcttcat cgccgagaaa
    36961 tacggcgtgc tgatcgatgc gctctacagc gggcgcgccg agcagttcat cgagaccgcg
    37021 gtcaaattcg aggacgggcg cgccggcaag gtgtcggcca cgctgaagat cctcgacgcc
    37081 cgcacctacc cgcccgtccg gagcgcggca tgaacacgat tgctactgaa acaatagctg
    37141 ccagcgctca ccagatgggc gctgcagccg gatccgatct gcaagccggg ggcaagaaga
    37201 tcggcgacgt gatcctcgac gtgcagaaca tcagcctgcg attcggcggc gtcaaggcgc
    37261 tgaccgacat cagcttcaac gtgcgcgagc acgagatccg ttccatcatc ggccccaacg
    37321 gcgccggcaa gtcgtcgatg ctcaactgca tcaacggcgt ctacacgccc agcgaaggca
    37381 gcatcacctt ccgtggcaag accttcagcc acatgaacag ccgccaggtc gccgagatgg
    37441 gcgtggcgcg caccttccag aacctggcgc tgttcaaggg catgagcgtg atcgacaaca
    37501 tcatgaccgg gcgcaacctg aagatcaaaa gcaacctgct gatgcaggcg ctgcgcatcg
    37561 gcccggccga gagggaagag atcgcacacc gcgagttcgt cgagcacatc atcgacttcc
    37621 tggagatcca ggcgcaccgc aagactccgg tgggccagct gccctacggc ctgcaaaaac
    37681 gcgtcgacct gggccgcgcg ctcgccatgg agccgcaggt gctgctgctg gacgagccca
    37741 tggctggcat gaacgtcgag gaaaagcagg acatgagccg cttcatcctc gacgtgaacg
    37801 acgaattcgg caccaccatc gtgctgatcg agcacgacat gggcgtggtg atggacatca
    37861 gcgaccgcgt ggtggtgctg gactacggca agaagatcgg tgacggcacg cccgacgaag
    37921 tgcgcggcaa cgaggacgtg atccgtgcct acctgggcac cagccactga gcgcgcgagg
    37981 aagcacaacc atgggattct ttctggaaac gctgttcggc ggcctgatga cgggcatgct
    38041 gtattcgctg gtggcgctgg gcttcgtgct gatcttcaag gcctcgggcg tgttcaattt
    38101 cgcccagggc gcgatggtgc tgttcgcggc gctggccatg gcgcgcctgg ccgagtggct
    38161 gccgcagtgg ttgggcttcg aaaaccgggt gctggccaac ctgctggcct ttgcgctggc
    38221 ggcgctgatc atgtggtccg tggcctggct gatcgagcgc ctggtgctgc gccatctggt
    38281 caaccaggaa ggcgccacgc tgctgatggc gacgctgggc atcacctact ttctggacgg
    38341 cctgggccag acgctgttcg gcagcgccag ctaccagatc aacatcggca tgcccaagga
    38401 cccgatgttc ctgttcgaga acgtcttcca gggcggcgtg ctggtgaaca aggaagacct
    38461 ggtggcggcg ctgatcgccg ccgcgctggt gaccggtctg accctgttct tccagaagac
    38521 caagaccggc cgggcgctgc gcgcggtggc cgatgaccac caggccgcgc agtcgatcgg
    38581 catcccgctc agccgcatct gggtcatcgt gtggacggtg gccggcgtgg tggccctggt
    38641 ggccggcatg atctggggca gcaagctggg cgtgcagttc tcgctggcca cggtggcgct
    38701 gcgcgcgctg ccggtggtga ttctgggggg gctgaccagc gtgccggggg ccatcgtcgg
    38761 cgggttgatc atcggcgtcg gcgagaagct gtccgaggtc tacctgggtc cgttcgtcgg
    38821 cggcggcatc gagatctggt ttgcctatgt gctggcgctg gcgtttctgc tgttccggcc
    38881 gcagggcctg ttcggcgaaa aaatcatcga ccgcgtttga catcgctcct gttccgatag
    38941 cttctggcgc gcgttgaata agccctgacc accgattcga gacccaccat gttctacaga
    39001 gaaaacggcc agttcaagac cagctaccga gccgaccagc agatcttccc gatcgcccag
    39061 gatcgctggg cggtgctgct gctggtggcc tgtgccttcg tggtggtgcc gttgctggcc
    39121 agcgaatacc tgttcctgtc ggtgctgatc ccgtttctga tcatggccat ggcggccatc
    39181 ggcgtgaaca tcctggtcgg ctactgcggc cagatttcgc tcggttcagg cgccttcatg
    39241 gcggtgggcg cctacgcggc ctacaacatg tatgcgcgca tcccgggcat gcagatgctg
    39301 gtggcggtgc tgaccggcgg tgcctttgcg gccctggtgg gcatcgtgtt cggcattccc
    39361 agcctgcgcg tgcgcggcct gtacctggcg gtggccacgc tggcggcgca gttcgtgtgc
    39421 gattgggcct tcctgcgcat cagctggttc accaaccaca gcacctccgg gtcggtgtcg
    39481 gtgtccaacc ttcatgtgct gggcctgccg gtggagacgc cggtgcagaa gtacctgttc
    39541 tgcctgagcc tgctggtggt gttcggcctg ctggccaaga acctggtgcg cagcgccgtg
    39601 gggcgcgaat ggatggccat ccgcgacatg gacgtggccg cggcggtgat cggcatccgg
    39661 cccatgtacg ccaagctgag cgcgttcgcg gtctccagct tcatcgtcgg cgtggcgggg
    39721 gcattgtggg ggttcgtcaa cctcggctcg tgggagccgg ccgccttttc ggtcgatcgc
    39781 tcgtttcagc tgctgttcat ggtgatcatc ggcggtctgg gctcgatcct gggttcgatc
    39841 ctgggtgccg cgttcatcat cgtgctgccg atcgtgctca atctgctgct gccctggctg
    39901 ggttcgctgt tcgggctcga ggtgtcgatc gccgccgtcg agcacaccca ggtgatgctg
    39961 ttcggcgcac tcatcgtctg gttcctgatc gtcgagccgc atggcctggc caagctgtgg
    40021 agcgtgggca agcagaaact gcgcatctgg cctttccccc actgaccggg caccacagcg
    40081 cccgcaaacc caaccaccgt ttttttccta ggaggcaaac catgaagttc cgtcaactga
    40141 cccttgccgc cgcggcggcc ctcacgctgg gcgcctcggc gtcggccatg gcccaggcgc
    40201 agcagttctt tccggtgctc agcggccgca ccgggccggt ggcgcccaac gccacgccct
    40261 gggccaacgg ccacaacgac tacatgaaac tggtcaacgc gcgcggcggc atcaacggcg
    40321 tcatgaccct ggtggaagaa tgcgaaaccg cctacgccac cgacaagggc gtggaatgct
    40381 atgaacgcct gaagggcaag cacggtggcg ccacggtgtt ccagccgctg tccaccggca
    40441 tcacgttcgc actgaccgaa aaggtgccgg gcgacaagat ctcgctcatc acctccggct
    40501 atggccgcag cgacaccgcc gatggcggcg tgttcaagtg gaacttcccg ctcatcggtc
    40561 actactgggt ggcgggcgac acggtgctgc agcacatcgc caagaaggaa ggcggctggg
    40621 acaagctcaa gggcaagaag ttcgccatcg tctaccacga cagcccgttc ggcaaggagc
    40681 tgctgccgat cgtgcaggag cgcgccaaga tgcacggctt cgagctgcaa ctgctgccgg
    40741 tgccggcccc tggtgtcgag cagaaggcga tctggctgca ggtgcgtcag cagcggcccg
    40801 atttcgtcat catgcagacc tggggcgtga tgacccccac cgccatcaag gaggccgtgg
    40861 ccaccggcta tccgcgcgag aaaatgttcg gcacctggtg gtcgggcgcc gagcctgacc
    40921 tgaaggacgt cggcgccgcc gccaagggct acaacgccgt catgatgcag cacggagccg
    40981 agccgcagtc cgccgtggtg aaggaaatcc tcgagaaggt gcacgccaag ggccagggca
    41041 ccggtcccaa ggaagaggtg ggcagcgtgc tgtacatgcg cggcgtgatg ggcgccatgc
    41101 tggcggtcga gggcgtgcgc gcggcccagg agcgcttcgg caagggcaag gtcatgaccg
    41161 gcgagcaggc acgctggggc tatgaaaacc tgaacctgac ccaggccaag ctggacgcgc
    41221 tgggcttcaa gggcgtgatg cgcccggtgt ccaccagctg ccaggaccac atcggttccg
    41281 gctgggcgcg cgtgcacacc tgggacggca gcaagttcgt ctggtcttcc gactggctgg
    41341 aggccgacca gcagatcatc aagccgatgg tcaagcagtc ggccgacaaa tacgccgccg
    41401 agaagaagct gacccgccgc accgccgccg actgcggcag ctgagccagc ggcaggagcg
    41461 agccgccgcc caccgcaggg tgcgcccgca cgcgcgtgcg ggtggcgctg cggcgctcgc
    41521 tgcaccggcg ttcgcccgtc gcgcacgggc gccattggag cctagatcat gaccaccgca
    41581 tccgccccca tcgtgctgac cgtcaacggc atcgaagtca tctacaacca cgtcatcctg
    41641 gtgctcaagg gggtttcgct gacggtgccg gaaagcggca tcgtcgccat cctgggcggc
    41701 aacggcgccg gcaagacgac cacgctgcgc gctgtctcca acctgctcaa gggcgagcgc
    41761 ggtgaagtga ccaagggctc gatcgagctg cgcggcgagc gcatcgagaa cctctcgccg
    41821 gccgagctgg tcaagcgtgg cgtggtgcag gtgatggaag ggcgccactg cttcgcccac
    41881 ctgacgatcg aggaaaacct gctgaccggc gcctatacgc gcagcgacaa gggcgagatc
    41941 gccgccaacc tggacaaggt ctacacctac ttcccgcgcc tgaagacccg gcgcaccagc
    42001 caggcggcct acacctcggg tggcgagcag cagatgtgcg ccatcgggcg cgccctgatg
    42061 tgcaacccca gcatggtgct gctcgacgag ccctccatgg ggctggcgcc gcagatcgtc
    42121 gaagaggtgt tcaacatcgt gcgcgacctc aacaccaagg agcgcaccac cttcctgctg
    42181 gccgagcaga acaccaacat ggcgctgaag tacgccgact acggctacat catggaaagc
    42241 gggcgcatcg tgatggacgg caaggccagc gatctggcca gcaacgagga cgtcaaggag
    42301 ttctacctcg gtgtcggcgg cggcgagcgc aagagcttca aggacgtcaa gagctacaag
    42361 cggcgcaagc gctggctggc ctgagcgtca tcctacaggc gtgcgccgca gtccggatat
    42421 ctgagtcaaa tcgaacgctg gcgcttgtga atgctgcgcc gacagctcca gtttttagag
    42481 cattcatcat gaccgaatac tttgacgcgc tcgaaacccg cgaccccgcg gcgcgtgagg
    42541 cggcgctgct ggcggcgctg ccccggcagc tggcgcatgc ccagcagcac agcgcggcgt
    42601 tcgccgacat tctggccggc atagatgccg ccgcggtgac ctcgcgcgcc gcgctggcgc
    42661 gcctgccggt gacgcgcaag agcgagctgc tggcgcgcca gcaggccagc cgcgccgctg
    42721 gccgtgaccc gttcggcggt tttgccaccg ggcgctgggg gtcggccatg cggcgcgtct
    42781 atgccagccc gggcccgatc tacgaacccg agggctgcgc cagcgattac tggcgcaccg
    42841 cgcgcgcgct gtacgcggcc ggctttcgcg ccggcgatct ggtgcacaac gccttcagct
    42901 accacatgac gccgggtgcc ttcatcctcg aagccggggc gctggcggta ggctgcacgg
    42961 tgtttccggc cggcaccggt cagaccgagc agcagttgca ggccatcgcc gagctgcggc
    43021 cgcaggccta cgtcggcacg cccagctttc tgcgcatcct gttcgagaag gcggccgagg
    43081 gcggcgccga catccgcagc ctgaccaagg ccatggtcag cgccgaaccg tttccaccct
    43141 cgctgcgcga ctggttcgcc gagcgcggca tgacggccta ccagagctac gccaccgccg
    43201 acgtcggtct gatcgcctac gagacggcgg cgcgtcaggg cctggtgctg gacgagggcg
    43261 tgatcgtcga aatcgtacgc cccggcaccg gcgaccctgt gcccgagggc gaggtgggcg
    43321 aagtggtggt cacggtgctg aacccggact acccgctgat tcgctttggc accggcgatc
    43381 tgtcggcggt gctggccggc ccgtgcccca ccgggcgcac caacacccgc atcaagggct
    43441 ggctggggcg cgccgaccag accaccaaga tccgcggcat gttcgtgcac ccgggccagg
    43501 tggccgagat cgtcaggcgt ttcccggggg tggggcgagc tcgcctggtg gtcagcggcg
    43561 ccatggccaa cgaccagatg acgctgcgcg tcgaagcggc cgacctgccc ggtctggccg
    43621 agcgcatcgc cgacgccgtg cgcgacgtca ccaagctgcg tggccagatc gagctggtcg
    43681 ccccgggcag tctgcccaac gacggcaagg tgatcgagga cgcgcgcagc tacgaataaa
    43741 ccgcggggat gcgctttttt ggcgcggcct cggggaattc ccggtgcgcg cggggctggc
    43801 ccaggcgttt cgaatcgata ccattggccg tttcattccc tcattgcaag gagctgctgt
    43861 catgtcaccc ctgttcaagc tgagcatcgc caccgtcgcc gccctggctg cgctgggcgc
    43921 ccaggcccag gccttcccgg ccgcgggcaa gaccgtcacc ctgatcgtcc cctttgccgc
    43981 cggcggcccc accgacaagg tggcgcgcga cctggccgag gcgctgcgca agccgctggg
    44041 cggtgccacc gtggtgatcg acaacgcggc cggcgccggc agcaccatcg gcgccgccaa
    44101 ggccttccgc gccgcaccgg atggctacac cctgctggtc acccacatcg gcatggccac
    44161 cacgccggcg ctgtaccgca agctgcccta caacgtcgag gagttcgagt acctgggcct
    44221 catcaatgag gtgccgatgg tggtggtcag caagccgggc ctgccggcca acaactgggc
    44281 cgagctgaag gcctggatcg gcagcaacaa gggcaaggtc aatctggcca acgccggcct
    44341 gggcgccgcc tcgcacctgt gcggcctgat gctgcaacag gcgctggcca ccgaaatgac
    44401 caccatcgcc tacaagggca cggcgccggc catgaccgac ctgatgggcg gtcaggtcga
    44461 catcatgtgc gaccagacca ccaacaccac cagccagatc gaggccaaga agatcaaggc
    44521 ctttgccgtc accacgccca agcgcatcac catgccgccg gtctacaagg acctgccgac
    44581 gctggaagag gtgggcatga agggcttcaa cgtctcgatc tggcacggcc tgtacgcgcc
    44641 caagggcacg ccggcggcgg tggtcaaggt gctcaacgac gccctgcagg cggcgctcaa
    44701 ggacgccgac ttcatcaaga aggaagaagc cctgggcgcg gtggtggcgc acgacgatcg
    44761 cgtgacgccg gccggccaca agaaattcgt caccgccgaa gtcgccaagt ggggtgcggt
    44821 gatcaaggcg gccgggcagt acgccgactg agaccgggcg gcgcggccca cgcccggccg
    44881 cgtggcgggc gtggcgccca ccaggcggca ccatgaaccg acctctgcgg ctggtggact
    44941 cgatcaccga gctcaggccc ggcgccgaca ccggctgcct ggcggtcagc ggctcgcacg
    45001 ggggcatcag ctcggcgcac tacgcgctgg cggcgcagcc gatgctggcc gtcttcaacg
    45061 atgccggcgt gggcaaggac gtcgccgggc tggcggccct gccgttcctg cagcggcacg
    45121 gcatcgccgc ctgcaccgtg gcccacacca gcgcgcgcat cggcgaggcg cgcagcaccc
    45181 tgcacgacgg tgtggtcagc cactgcaacg cccaggcgct gagcctgggc atcgagccgg
    45241 ggcaggcgtg cgccgcgctg atccggcaac ttcaaggctc tggaggatga acatggcaca
    45301 aggtttcaaa gcaaatcggc gtctggcgct tgccgggatt gcgctggcag ctacgcaatt
    45361 tctagcaaac atggcctggg cacagggcgc ctggccgagc aagccggtca ggatcgtggt
    45421 gccgttcgcc cccggcggca ccaccgacat cctggcgcgc gccctggcgc cggagctgag
    45481 caaggcgttc ggccagcagt tcatcgtcga caaccgcgcc ggtgccggcg gcaacgtcgg
    45541 ggccgaggtg gtggccaagt cgccgggcga cggctacacc ctgctgatgg gcacggtcgg
    45601 cacgcacggc atcaaccgtg cgctctaccc caggctgccc tatgacccga tcaaggactt
    45661 cgcccccatc accctggtgg ccagcgtgcc caacgtgatg gagatgaacg ccgacaaggc
    45721 ggcgcagctg ggcatccaca acgtgcagga cttcatccgc tacgtcaagg cccaccctgg
    45781 caggctgaac atggcttcca gcggcaacgg cacctcgatc cacctggccg gcgagctgtt
    45841 caagcacatg accggggtct tcatgaccca cattccctac cgtggctccg gcccggcgct
    45901 gctcgacatg gtggccggca acatggacgt gatgttcgac aacctgccat cgtcgatggc
    45961 gcagatcaag gccggcaagc tcaagccgct ggccgtgacc agcgcccggc gctctgccgc
    46021 cctgcccgac gtgccgacgg tggaggaggc cggtggcccg gccctcaagg gttacgaggc
    46081 cagcagctgg ttcgggctgc tggcgccggc cggcacgccg gccgatgtgg tgagccgact
    46141 gcagcaggaa tcggccaagg cgctggcctc accggccgtg aaggaaaagc tgctggccca
    46201 gggcgcggtg ccgggtggca ttccgcccgg cgaattcgcc ctcatgattg ccgacgaaca
    46261 cgccaagtgg gccaaggtgg tcaaggcctc gggcgccaag gtcgattgag cgcggcggcg
    46321 gcccgggccg gccgctcgat atgcggggtg gggccggagg gccgcgccgt cagacccccc
    46381 acaccacgtc tttgccggcc gcgctgctga tctgcagcag ctcgatgaac ggcgcggcgc
    46441 gctgacgcag cgtcaccagc tcctggcgtg caaattcatc ggcggtggcc tgcgtgcgcg
    46501 cctcgtcgtc gctggtcggt gcggcgtgcc cctgtccctg cgcctcgcgg cgctcgatct
    46561 cggccttcag ggcggcgatc gcggccggcg cctgatccac ggtgacgatg ccggtcgggc
    46621 tcgcgggttt gccgatgatc ttgagcagct cttcggccga ggccttgagc atgatgacgt
    46681 cggcggcggc ctgcgacttg aatttgtaaa gcatggctgg ggtctccggt tgatggcgcg
    46741 cccatggcgc ctgtatccga ttgtgccccg tccgggtaac taccaagccg cccgacgtca
    46801 aatcctcttg acctgattta gttgagacat aaactatttt tgcatcaacc aaatcggctg
    46861 tggctcagga gacggagcat gaagattgtg tgcatcggag gcggaccggc gggcctgtac
    46921 ttcgcactgc tgatgaagcg gctcaacccg gcgcatgaca tcacggtgat cgagcgcaac
    46981 cggccctacg acacctttgg ctggggggtg gtgttttccg atgccaccat ggacaacatg
    47041 cgccagtggg acgcccccac ggccgacgcc atccagcagg ccttcaacca ctgggacgac
    47101 atcgagctgc tgttcaaggg gcaggtgatc cgctccggcg gccacggctt cgtcggcatc
    47161 ggccgcaaaa agctgctcaa catcctgcag gcgcgctgcg aggcgctggg cgtgcggctg
    47221 gtgttcgagc aggaggtcga cgacgatgcc gcgtttgccg acgccgacct gatcgtggcc
    47281 agcgacggca tcaactccag aatccgcacg ctgcatcagg cgcagtttcg acccgaactg
    47341 gtggtgcgcc cgaaccgcta catctggctg ggcacccgca agctctacga cgccttcacc
    47401 ttcattttcg agaaaaccga acacggctgg ttccaggccc acatctacaa gttcgaccaa
    47461 cacacctcca ccttcatcgt cgaatgcccc gagccggtgt ggcgggcgca cgggctggac
    47521 caggcgacgc aggaggcgtc ggtggcgttc tgcgaaaagc tgttcgcccg ccacctgcag
    47581 ggggagcgcc tgatgaccaa cgcgcgccac ctgcgtggct cggcctggct caacttcacg
    47641 cgcgtcaagt gcgaccgctg gtggctgcgc aacgccaacg gggcccatgt ggtgctgatg
    47701 ggcgacgccg tgcacacggc gcatttcgcc atcggctcag gcaccaagct ggcgctggag
    47761 gacgccatcg ggctgacgcg cgagttcaag cgcctgggcg acagcccggc acagatcgag
    47821 caggtgctaa gcgactacca ggcgtcgcgc agcgtcgagg cgctgcgcct gcagaacgcc
    47881 gcctggaacg cgatggagtg gttcgaggtc tgtggcgaac gctactgcga ccagctggag
    47941 ccgccgcagt tcatgtattc catgctcacg cgcagccagc gcatcagcca cgagaacctg
    48001 cgcctgcgcg accggcagtg gctggaaggc tatgagcgct ggttcgctag gcgcgacggt
    48061 ctggccgtgg ccgatgccgc ccggccgccg ccgccgatgt tcacccccta caccgtgcgt
    48121 ggtgtgacgc tgaagaaccg cgtggtggtg tcgccgatgg cgcagtactc ctgcgtcgac
    48181 ggcgtgccgg gcgactatca cctggtgcac ctgggcgcac gcgccctggg cggcgccggc
    48241 ctggtgatgg ccgagatgac ctgcgtcagc cccgaaggcc gcatcacccc cggctgtccc
    48301 ggcctctaca acgccgagca gaaagccgcc tggcagcgca tcgccgactg gatccacgag
    48361 cacagcgacg ccaggttcgg catgcagatc ggccacgccg gccccaaggc ctcgacccgc
    48421 ctggcctggg acggcaccga cctgccgctg ccgcagggca actggcccat cgtctcggcg
    48481 tcgccgcagc agtacctgcc gggcatcagc cagcgggccc gggcgctgac gcaggccgaa
    48541 atgcaggtca tcctgcagca gttcgtgcaa gccgcgcaga gcgcggccga catcggcgtc
    48601 gactggctgg agctgcactg tgcgcacggc tacctgctgt cggccttcat ctccccgctc
    48661 accaaccagc gcacggacga atacggcggt agcctggaaa accgcctgcg gtttccgctc
    48721 gaagtgttcc gggcgatgcg tgccgtgtgg ccgccgcagc ggccaatgtc ggtgcgcatc
    48781 tcggccaccg actgggtcga aggcggcacc acgccggccg atgcggtggc catcgcgcgc
    48841 gccttcaagg aggccggtgc ggatctgatc gactgctcca gcgggcaggt cagcaagcag
    48901 gagcaaccgg tgttcggccg catgtggcag acgccgttcg ccgaccgcat tcgccaggag
    48961 gcaggcatcg ccaccatggc cgtcggtgcc atcagcgaag ccgaccatgt caacagcatc
    49021 atcgccgccg gccgcgccga cctgtgcgcg gtggctcggc cgcacctggc caacccggcc
    49081 tggaccctga ccgaggcggc ccgcatcggc tacgccgaca tgacctggcc acgcccgtat
    49141 ttccagggca agcgccagct cgaagccggg ttcgaacgcg cccgggccgc cggcggcgcc
    49201 ccgatctgag gaagcgacga tggacctgga agcgcgcgcc cacgccgaac accccgaagc
    49261 gctgcgcctg tggctgcgcc tgctgacctg cacccagatg gtcgagcgcg atctgcgcaa
    49321 ccgcttgcgc gaggcgttcg acaccacgct gccgcgcttc gacctgatgg cgcagctcga
    49381 acgcgtgccc gagggcctgc gcatgaacga gctgtcgcgc cgcctgatgg tcaccggcgg
    49441 caacgtcacc ggcatcaccg atcagctgga ggccgaaggc ctggtcgagc gcgtgccggt
    49501 cgaaggcgac cggcgcgcct ttcatgtgcg gctcacgccg gccggccgcc agcagtttca
    49561 ggccatggcc caggcgcacg agcaatggat cgtcgatgcc ttcgccggtc tgacctcggc
    49621 cgagatccgc accctgcacc agctgctggg ccgggtgaag caacattttc tggagaccac
    49681 gtgaagcact acatcccttc agccaacccg atgcggccgc agtaccgggc gcaggcggcc
    49741 taccaggcgc gccactttgc ctggcagtgc gacgccgacg gcgtggccac cgtcacgctc
    49801 gatcgtcccg agcgcaagaa cccgctcacg tttgacagct acggcgagct gcgcgatttg
    49861 ttcaatggcc tgcgccacgc caccgacgtc aaggtggtgg tggtgacggg ggcgggcggc
    49921 aacttctgct cgggcggcga cgtgcacgac atcatcgggc cgctgaccaa gatgagcatg
    49981 cccgagctgc tggagttcac gcgcatgacg ggtgatctgg tcaaggccat gcggggttgc
    50041 ccgcaacccg tcatcggcgc catcgacggc gtgtgcgccg gcgccggcgc catgatggcg
    50101 ctggcctgcg acctgcgcta cggtacggcg gcgaccaaga cggcctttct gttcactcgc
    50161 gtcggcctgg ccggcgccga catgggcgcc tgcgcgctgc tgccgcgcgt gatcggccag
    50221 ggccgcgcca gcgagctgct gttcaccggc cgcgcgatga gcgccgacga aggcctggcc
    50281 tggggttttt tcaatgcctt gcacggcagt gaggaactgc tggccaaggc ccaggccatg
    50341 gcgggccagc tggccgcagg gcccatgttc ggccacgcca tgaccaagaa catgctgcag
    50401 caggaatggg ccatgacgat agatcaggcc atcgaagccg aagcccaggc ccaggccatc
    50461 tgcatgcaga cccaggactt ccgccgcgcc tacgaagcct ttgccgccag gcaaaagccg
    50521 gtgttcgggg gtgactgatg agcaccggca tcctgcaacc cacggccttg cggccgtcca
    50581 ccagtcacct gggcctgccc ttcttcgatg aagcgcatcg gcgcattgcc gccgagttgc
    50641 ccgactggac caatgcccag ggtgtggacg aaagcgacga ccgcgccgcc tgccgcgact
    50701 gggtgcggcg cctgggcgcg ggcggctggc tgcgctactg cgtgccgggc gactgcggcg
    50761 gcgcgctgcc ccagctcgat tcgcgcgccc tggtgctgct gcgcgagacc ctggcctttc
    50821 attcgccgct ggccgacttt gcctttgcca tgcagggcct gggcagcggg gcgatttcga
    50881 tcggcggctc ggccagccag cgccaggcct atctgccgca ggtggcccgc ggcgccagga
    50941 tcgccgcctt tgccctgtcc gagccggagg ccggctccga cgtcgctgct atgcaattaa
    51001 gagctgctgg cgcgcgcccc gattgcgtcg caggccaaaa ggacgactca tatttgctgg
    51061 acggccgcaa gacctggatc agcaatggag gcatcgccga cttctactgc gtgtttgcca
    51121 agaccgaccc cgcgggcggc agccgcggca tcagtgcctt catcgtcgat gccggcacgc
    51181 cgggcctgga cgactcggcc cacatcccgg tgatggcgcc gcacccgctg gccaccctgg
    51241 tgttcgagcg ctgccgcgtg gcgggcgagc agatgctggg cgagttgaat ggcggcttca
    51301 agctggccat gcagacgctg gacatcttcc gcgccagcgt cgccggcgcg gccctgggca
    51361 tggcgcggcg cgcgctggcc gaggccatcg ctcatgcgcg ccagcgccgc atgtttggcc
    51421 agaccctggc cgactttcag ctcacgcagg ccaagctggg cgagatggcg gcgctgatcg
    51481 acggggcagc gctgctgacc taccgcgccg cctggctgcg cgacaccggg caaggcagcc
    51541 ccaaggagcg cagcgtggcc gccgccatgg ccaagatgac cgccaccgaa aacgcccagc
    51601 gcgtgatcga catggcgctg cagatgcacg gcggcctggg cgtgatggtg ggccgcaagg
    51661 tcgagtcgct gtaccgcgac attcgcgccc tgcgcattta cgagggcgcg accgaagtcc
    51721 agcaactcat catcggcaag agtgtgctgc aggaggcatg accatgagcg tcccatccgc
    51781 ccagaccgac cgctacgtgc acgatcgcct gccaccgcgc gagcagtggc ccgagttccg
    51841 ctacgacctg ccggagctgc gcattccgga tcaggccaac ctggtcaccg tcctgttcga
    51901 cgccgcccgc gccaacggct ggagcaaccg cctgctgctg tgctcacccg gccagacgct
    51961 gacctacgcc gaggccgagg ctcaggttgc gcgcatcgcc gagatgctga tccgggactt
    52021 caagctcgag cccggcaacc gcgtgctgct gcgcggtggc aactcgatcg gcatggcgct
    52081 ggcctggctg gcggtggtca aggccgggct gatcgtggtg cccaccatgc cgctgttgcg
    52141 cgcgctcgag ctgggcaagg tgatcgacaa ggcgcagccg gcgctggcgc tgtgcgaggt
    52201 cgatctgctc gaggacatgg tgctggcggc gcaggacgcc cggggcaggc tgccgatcct
    52261 ggcgttcggc gatcccgggc gggccgacgg gctggagcag cgcgccgcgc gtcgttcgcc
    52321 cgaccacgcg ggctgcgcca ccgccgccga cgacattgcc ctgctggcct tcaccagcgg
    52381 caccaccggc gtgcccaagg cggtggtcac cacgcaccgc gacatcctgg ccgcctgcaa
    52441 cacctggccg cgccacgtgc tgaagatgac gcccgacgac atcgtcgccg gctcgccgcc
    52501 gctggccttc accttcggca ccggggggct gttgatctat cccatgtggg caggcgcctc
    52561 ggtgtactac cccgacgtgc gcttcagccc cgagaccatg gtgcgcaaga tgcgcgacaa
    52621 cggcatcacc gtgtgctaca ccgcgcccac cttctaccgc cagatggcgc cctatgccaa
    52681 ggcgctgggc attcccagcc tgcgcatctg cgtcagcgcc ggcgaggccc tgcccgatgc
    52741 cacgcgccag ctctggaagg acgccaccgg catcgagatg ctcgacggca tcggcggcac
    52801 cgagatgtgg cacatctaca tctccgcggc gggcgccgac gtgcgcccgg gcacgatcgg
    52861 caaggtggtg ccgggctacc aggcgcgcgt ggtggacgag cacgaccagg aagtgccacg
    52921 cggcaccgtg gggcggctgg ccgtgatcgg ccccaccggc tgccgctaca tggacgatgc
    52981 gcgccagacc cgctacgtcg cccatggctg gaaccacccc ggcgacgcct gcatccagga
    53041 cgaggacggc tacttccact accaggcgcg cgacgacgac atgatcgtca ccgccggcta
    53101 caacgtcggc ggccccgagg tcgaggactg cctgctcacg catccggcgg tggccgagtg
    53161 cggtgtgatc ggcaagcccg acgccgtgcg cggcatgatc atcaaggcct tcgtggtgct
    53221 caggcccggc cagccggaca acgaggccat ggtcgaggcc ctgcaggcgc atgtgcggca
    53281 gcacatcgca gcctacaagt acccgcgcga aatccagttc gtgacggcgc tgccacgcac
    53341 cgacaccggc aagctgcagc gcttcaagct gagggagctg gcatgaaccc gcaacggatc
    53401 caccccgagg gctgggcgcc ggccaagggc tacgccaacg gcgtggccgc gcgcggcacg
    53461 cagatctatg tcggcggtca gatcggctgg aacgcgcagc agcagttcga gagcgacgac
    53521 ttcatcgcgc aggtgcacca ggccttgcgc aatgtcgccg cggtgctgca agccgccggc
    53581 gccggccccg agcacatggt gcgcatgacc tggtacgtgc tcgaccgcga cgaatacaac
    53641 cgccggctga aggaactggg cggcgtttac cgcgacgtca tgggcaggca ctttccggcc
    53701 atgagctgcg tgcaggtggc ggggctgatg gaggcgcgcg ccaaggtcga gatcgaagtc
    53761 acggcggtca ttcccgactg atggacaatc ctcatccgcc ttaccccgtg gaacccgcca
    53821 tgtccaagac caagaccccc ttcagctggc aagacccgtt tgccctgcac gatcagctca
    53881 gcgccgacga gcgcgcggtg caggacgccg cccacgccta ttgccaggac aagctgctgc
    53941 cgcgcgtgca gcaggccttc cgcagcgagc agaccgaccc ggcgatcttt cgtgaaatgg
    54001 gcgaactggg cctgctgggc cccaccatcc ccgagcaata cggcggcgcc ggcctgggct
    54061 acgtcagcta cggcctgatc gcgcgcgagg tcgagcgcgt ggactcgggc taccgctcga
    54121 tgatgagcgt gcagtcttcg ctcgtcatgg tgccgatcaa cgaattcggc aacgaggcca
    54181 ccaggcagaa atacctgccc aagctggcgc gcggcgaatg gatcggctgc tttggcctga
    54241 ccgagcccaa ccacggctcc gaccccggca gcatggtcac gcgcgccaag aaggtgcccg
    54301 gcggctattc gctgtcgggc agcaagatgt ggatcagcaa cagcccgatc gccgacgtgt
    54361 ttgtggtctg ggccaaggac gacgagggcg cgatccgcgg cttcgtgctc gagaaaggct
    54421 ggaagggcct gtccgcgccg gcgattcacg gcaaggtcgg cctgcgcgcc agcatcaccg
    54481 gcgaggtggt gatggacgag gtgttctgcc ccgaggaaaa cgccttcccc gaggtgcgcg
    54541 gcctgaaggg cccgttcacc tgcctcaact cggcgcgctt cggcattgcc tggggcgcgc
    54601 tgggcgcggc cgaggactgc tacgcccgcg cgcgccagta cgtgatcgac cgcaagcagt
    54661 tcggcaagcc gctggcggcc aaccagctga tccagaaaaa gctggccgac atgctgaccg
    54721 agatcagcct cggtctgcag gcctgcctgc gcctgggccg catgaaggac gagggccacc
    54781 cgccggtgga gctgacctcc atcatcaagc gcaacagctg cggcaaggca ctcgacatcg
    54841 cccgccaggc gcgcgacatg atgggcggca acggcatcag cgacgagttc ggcgtggcgc
    54901 gccacctggt gaacctggag gtggtcaaca cctacgaggg cacgcacgac atccacgcgc
    54961 tgatcctggg cc
//
LOCUS       DAOQTZ010000007        49589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_787_length_49589_cov_14.388137, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000007 DAOQTZ010000000
VERSION     DAOQTZ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 49589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49589
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_787_length_49589_cov_14.388137"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKN51_01745"
     CDS             complement(<1..209)
                     /locus_tag="PKN51_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83851.1"
                     /translation="MQRQQALEALLLNLLPADHSTVGNATLQQQFLAAAQAAGHPGPV
                     TADDFQAAREALLAAGLAVKGKGRGG"
     gene            322..>1346
                     /locus_tag="PKN51_01750"
     CDS             322..>1346
                     /locus_tag="PKN51_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNT83852.1"
                     /translation="MTAPAYTRAAALPAILRQRLVILDGAMGTMIQQLKLSEAQFRGE
                     RFADFAKDVKGNNELLSLTRPDVIQAIHEKYLAAGADLVETNTFGATTIAQADYGMEA
                     LAREMNVASARLARAACDKYSTPDKPRFVAGALGPTPRTASISPDVNDPGARNISFEE
                     LRAAYREQAEGLIEGGADVLLVETIFDTLNAKAALFAIDEAFEHSGESLPIIISGTVT
                     DASGRVLSGQTVTAFWYSVRHARPLAVGLNCALGAALMRPYIQELARAAPDTFISCYP
                     NAGLPNPMSDTGFDETPEVTSRLLHELAAEGLVNIVGGCCGTTPEHIGAIARAVGPLA
                     PRGVQAAG"
     assembly_gap    1347..1446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1620..2501)
                     /locus_tag="PKN51_01755"
     CDS             complement(1620..2501)
                     /locus_tag="PKN51_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT83853.1"
                     /translation="MTTLTRRQLAGLLLLTLMWGLNWPIMKLSLRELAPLHFRALTMG
                     LGALVLAAYFAARGLRLWPRGTAEWRDVLVLGLPNILGWHALSIIGVAHLPAGRAAIL
                     GFTMPVWTVLLSVLFYGERLSARLLLAVGAVLAGITLLLWGELAQLAGRPAGIAWMQG
                     AALAWALGTIWMRRAHLTLPAESLVVWMMAWSALVLALLAWWLEPPQRASLSAAVWLS
                     LLWSVLINYGAAQVIWFALARALPAATSAMSVMAVPLVGTLSAPLIVGEWPGWHDLAA
                     MVCVVVAIGTVLLRPNR"
     gene            complement(2539..3489)
                     /locus_tag="PKN51_01760"
     CDS             complement(2539..3489)
                     /locus_tag="PKN51_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNT83854.1"
                     /translation="MKTVTFRQLRVFVEVARHLSFVRAAEALHLTPPAVTMQIKELES
                     AIELPLFNREGRKVSLTTVGEYFLVYAKRLLATLKDADDAMARFRKLESGALSIGLVS
                     TAKYFVPQLLTRFRDEHPGVELKLLVGSNREQLVAMLQQGDVDLAIMGRPPKEMATRA
                     EPFAAHPHVFVAPPRHPILAVSQPSLAALARFPLIVREAASGTRALMDRFFQDHRVEP
                     RIAMEMPSNETIKQAVMAGMGLSFLSLHTIGLELRCGLLERVPVEHTPLVRTWNVVHM
                     QSKLLSPAAEALRYFILEHGEAHLAAHDRALLAADSAPSV"
     gene            3582..4277
                     /gene="rpe"
                     /locus_tag="PKN51_01765"
     CDS             3582..4277
                     /gene="rpe"
                     /locus_tag="PKN51_01765"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNT83855.1"
                     /translation="MDHRIAPSILSADFARLGEEVRDVIAAGADWIHFDVMDHHYVPN
                     LTMGPAVAQAIKPHCKRADGTPVPLDVHLMVQPVDALAAAFAQAGADLITFHPDASTH
                     VDRTLQLIRAAGCQAGLAFNPAAPIDVLEWVIDRVDLVLVMSVNPGFGGQTFIDGTLP
                     KLRRLRQWIDAEHERSGRTIRLEVDGGIKADNIRRVADAGADTFVAGSAIFGQPDYRA
                     VIERMRAELERAR"
     gene            4322..5410
                     /locus_tag="PKN51_01770"
     CDS             4322..5410
                     /locus_tag="PKN51_01770"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNT83856.1"
                     /translation="MPLSERQTLTQYLIEQRRRFPGASGELNALLLDVAIACKGIARA
                     VAQGELGADPASANADAGDVNVQGEVQKRLDVISNQMFLRQTEWSGNLAGMASEEMAQ
                     PYPIPARYPRGKYLLVFDPLDGSSNLDVNGAVGSIFSILRAPPEVVGAHRDLTEADFL
                     QPGTCQVAAGYALYGPVTMLVLSVGSGVVGFTLDAHIGEFKLTHPNIAVPEDANEFAI
                     NTSNSRFWEPPVKRYIDECLAGRSGPRGKDFNMRWIASMVAEAHRILMRGGVFMYPRD
                     TKDPSKPGRLRLLYEANPIGFIMEQAGGRASTGRQNVLGVAPSSLHQRIGLVFGSRNE
                     VERIERYHQEPARKAPAPLFAERSLFRE"
     gene            5436..6320
                     /locus_tag="PKN51_01775"
     CDS             5436..6320
                     /locus_tag="PKN51_01775"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNT83857.1"
                     /translation="MSERNPIIAITGSSGAGTSSVTRTFENIFRREGVTAAVIEGDSF
                     HRYDRKEMKARQAEAERNGNKHFSHFGPENNLFSELEQLFRSYSETGTGRRRKYLHDP
                     VEAAPYQQEPGTFTPWEPLPEGTELLFYEGLHGAVVTPEVNVARYPDLLIGVVPVINL
                     EWIQKLYRDKNVRGYSAEAVTDTILRRMPDYVNYICPQFAHTHVNFQRVPVVDTSNPF
                     IARDIPAADESICVIRFANPKGIDFPYLLNMIHNAWMSRANTIVVPGGKMDLAMQLIF
                     TPFIWRMMERRRHAMGGL"
     gene            6320..8362
                     /gene="tkt"
                     /locus_tag="PKN51_01780"
     CDS             6320..8362
                     /gene="tkt"
                     /locus_tag="PKN51_01780"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNT83858.1"
                     /translation="MAAHATERVVRGSAGCSDPRANALRALAIDAVQQASSGHPGAPM
                     GMAEMAVALWARHLRHSPANPQWPNRDRFVLSNGHASMLLYALLHLSGYDLPVEEIRR
                     FRQLHSRTPGHPEVGITPGVETTTGPLGQGIGNAVGMALAEQMLAREFNRPGHTIVDH
                     HTYAFVGDGCLMEGVSHEACALAGVWGLGKLIALYDDNGISIDGPVHGWFRDDTPSRF
                     RAYGWEVIGPVDGHDIGAVEQAIAQARAGADRPTLIVCRTTIGQGSPARAGTARAHGE
                     PLGAEEVVATRAAIGWTADAFDVPPAVRAQWDATTAGRAHEAAWQAKFDAYRDAHPAL
                     AAEFERRVRGELPATFGATLHAAIETLGATTDKVASRKASQQVLGLLAPKVPEMFGGS
                     ADLTGSNLTDFPGADQRHLSYGVREFGMAAIMNGVALHGGFIPYGGTFLTFSDYSRNA
                     IRMAALMKQRVIHVFTHDSIGLGEDGPTHQAVEHVPALRLIPHLDVWRPADNVETAVA
                     WGAALQRRDGPTVLALSRQGLPQLTRPTQAEAMARGGYVFAEPPQELPAQAVIIATGS
                     ELQLARQAQQLLWAEGLPARVVSMPSTSVFDRQDAAYREHVLPSQLPTVAVEAAHPDF
                     WRKYVGRRGGVLGVATFGESAPAEDLYRHFGLTAERVAAAVRACVRAAAPGGGAGS"
     gene            8362..9027
                     /gene="gph"
                     /locus_tag="PKN51_01785"
     CDS             8362..9027
                     /gene="gph"
                     /locus_tag="PKN51_01785"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548220.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNT83859.1"
                     /translation="MRAVLFDLDGTLMETAPELTDALNDTLRRHDLPAVDEARVRGWI
                     GDGARALLAKAFAHAGATPAAANAAWPGFHRDYSARCGTRSSLFPGARELLARLSKAG
                     IRTALLTNKESAFAHRLLARHDLASAFDLIVAGDTLAVKKPDPGVVMHALQKLHIEPG
                     QALLLGDSLTDVRTARAAGIAVRIVRHGYPQGELAGADAPDAFIDHFDDFDPGACRHA
                     PAT"
     gene            9055..10092
                     /gene="fba"
                     /locus_tag="PKN51_01790"
     CDS             9055..10092
                     /gene="fba"
                     /locus_tag="PKN51_01790"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348752.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNT83860.1"
                     /translation="MALVSLRQVLDHAAEHGFGVPAFNVNNLEQIQAIMEAAQATDSP
                     VILQASAGARNYAGEAYLRHMVLAAVETHPDIPVVLHQDHGASPAVCMQAIRSGFTSV
                     MMDGSLQEDARTPASYAYNVDVTRRVCAMAHAVGVSVEGELGCLGSLETGEAGEEDGS
                     GAQGKLSHDMLLTDPAQARDFVAATGVDALAIAIGTSHGAYKFSRQPTGDMLAIDRIA
                     SIHAAIPHTHLVMHGSSSVPQEWLAIIRKHGGTIKETYGVPVEEIQRGIAHGVRKINI
                     DTDIRLAMTGAMRRLFAEQPAEFDPRKAMAEARRAAKGICQARFEAFGCAGHAGRMRP
                     IPLDAMALRYR"
     gene            10224..10970
                     /locus_tag="PKN51_01795"
     CDS             10224..10970
                     /locus_tag="PKN51_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNT83861.1"
                     /translation="MLRALIWDVDGTVAETEADGHRIAFNQAFEACGLDWHWDLPTYG
                     QLLEVTGGRERLLAYMDRRDDAPASPAGRAALARAVHLRKNAFYADLVARGGIPARPG
                     VRRLMDECQRQEVRLAIATTTSTSNVEALFSSLFGPTWRDRFAAIVCAEDAPRKKPDP
                     QAYLLALRRLGVTPQEAFAIEDSPNGLRAARAAGLRCGITCSRYFADAGFEGAAWLRP
                     DLETPRPMTLQTLQALDGAAESETRPRGRP"
     gene            complement(11040..13370)
                     /locus_tag="PKN51_01800"
     CDS             complement(11040..13370)
                     /locus_tag="PKN51_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83862.1"
                     /translation="MSQTARFDAYGKAPPPPAAAASRHAALRERLDCRFPAIDDVFDD
                     CIDEALRTLTPAGMAAYLDQARWFGKLGRGAQPVLQFLAAWPAVARCCGESSLAPLCA
                     AAAAFQKSPNGPAIVTLLHSLPAVARQLPAPHLLAQYLALTVELMQRTSLSIHGRHAT
                     EPSPGLPAFLQQAGVLVALVPMEGLRRWVQEGIRLHGAHPQRQIDYFSLTSADARALL
                     QRERRGTLLIDVERRLALGLRGLWDVALPLVPYPVHVDHPTGQPLLPHLQDDGLRLPE
                     VLDDRAGVSALQRYRAMLAHLAGHLRWSTPQLADNLSPLQRLALECFEDARVDHLVLR
                     RYPGLRPALLALHPMPAEDACDAARVSCLRHRLVCLSRALLDPAHPYRDATLLAFAGR
                     FRAALRDGVSSTREMAVLALDWSAATRRASDQRARVQFEDTLVDWRDDNRHLWTFIEP
                     GDEEDTGAEPRRRAPVADEGGLPPRLYPEWDHLTRGHRPDWVSVYDILQPPGQAGDVD
                     ALLAQYAPVARHLKRRIDLLKPQDRTRLRHQEEGTELDLDLALRAIADWRAGAQPDPR
                     IHTSTRTDGRDISVLLLMDLSQSVNQTVGQPHTGAPAGGAPTVLSLSRAAAALLGWAI
                     AALGDELAIAGFQSNTRQEVRYLHLKGFTEAWGDAPKARLAAAQGAWSTRMGAALRHA
                     GHLLSGRRADKRLLLVLTDGEPADIDVDDPQYLIADAAQAVRELDRQGLYTHCISLDA
                     RADAYVSRIFGQRWTVIDRVEQLPRRLPELFLALTR"
     gene            complement(13517..14320)
                     /locus_tag="PKN51_01805"
     CDS             complement(13517..14320)
                     /locus_tag="PKN51_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNT83863.1"
                     /translation="MNPLADWRVAAEPFYAPQGGEIELFEAAYAARLPVMLKGPTGCG
                     KSRFVEHMAWKLQRPLVTVACNEDMTAADLVGRFLLEPDGTRWQDGPLTTAARHGAIC
                     YLDEVVEARQDTTVVIHALTDHRRALPLDKKGELVRAHADFQLVVSYNPGYQSLMKDL
                     KTSTRQRFAALDFDYPEAALEARIVVAETGIDAAVAAALVQVAGAARRLKGHGLDEGI
                     STRLVVYAATLIRQGIDARAACRMAMVRSITDDTDIRESLDHAIDAVFE"
     gene            complement(14443..15825)
                     /locus_tag="PKN51_01810"
     CDS             complement(14443..15825)
                     /locus_tag="PKN51_01810"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HNT83864.1"
                     /translation="MDQSNRYADLSLREEDLIRGGKHILVAYKMRPKAGHGYLAAAAH
                     FAAESSTGTNVEVSTTDEFTKGVDALVYLIDEVSEEMRIAYPLDLFDRNLIDGRMMIV
                     SFLTLAIGNNQGMGDIEYAKMYDFWVPPRALQLFDGPSKDISDLWRVLGRPLKDGGYI
                     AGTIIKPKLGLRPEPFAQAAYEFWLGGDFIKNDEPQGNQVFAPMKQVMPLVRDAMKRA
                     MDRTGEAKIFSANITADDHSEMLARGEYILETFGPDADKVAFLVDGYVGGPGMVTTAR
                     RWFPNQYLHYHRAGHGAVTSPQSKRGYTAYVLAKMSRLQGASGIHVGTMGYGKMEGEK
                     DDRAIAYIIERDSYTGPAYHQEWLGMRPTTPIISGGMNALRLPGFFENLGHGNVINTA
                     GGGSYGHIDSPADGARSLRQAYECWKAKADPIEWAREHREFARAFDSFPGDADKLYPG
                     WRDKLAGVHR"
     gene            complement(16022..17263)
                     /locus_tag="PKN51_01815"
     CDS             complement(16022..17263)
                     /locus_tag="PKN51_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HNT83865.1"
                     /translation="MHPSGPHPLWFCLALALPAMASAQSPAMPAGAERIDAATAWQAC
                     LGQPDAQRLACFDGWAHGQRQLIEAIAQKAQSVEATPTRNAAPSAQAAAVRGELAQAL
                     ATTQPERADGALVSGSAGIVGVGLEQGCRDRQFSDLSRFWELESGSSCPTFGLRAFHP
                     TTASIAAGDHINRLPTSPNPANSAITPIDYGKREMRLALSVRTKLAGGLLTPAGGTLR
                     DSLWAAYTQQSYWQVFNGSLSRPFRNTDHMPELIYVYPTTLKLPGGWLWRYSGIGLAH
                     QSNGQSDPLSRSWNRAYLMAGFERGNALNLQLRLWKRLPEDSAKDNNPDIVRHIGRGD
                     LSLAWNVDAKNTLRATYTGSFNRYGSGRLEWTRSLGDGWGNSFSSLRLYTALFHGYGD
                     SLLDYNFKRTVFSIGLSLVDF"
     gene            17521..17964
                     /locus_tag="PKN51_01820"
     CDS             17521..17964
                     /locus_tag="PKN51_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HNT83866.1"
                     /translation="MTPSPLLFPLQGALRRVLYVGLYEALAIAVVTVAFVAGDRSLGS
                     ASGVAVGSSLIAVLWNLVFNAAFERWESRQRVRGRSRARRLAHAIGFEGGLALWLVPF
                     MAWWLEVGLWQALLLDLGLLLFFLAYTFVFTWVFDRLFGLPRAAA"
     gene            17977..19182
                     /locus_tag="PKN51_01825"
     CDS             17977..19182
                     /locus_tag="PKN51_01825"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNT83867.1"
                     /translation="MLELADIQQAAERVRGQVLDTPCVESRTLSQLTGTQLFLKMENL
                     QFTASFKERGACNKLAQLTDQEKARGVIAMSAGNHAQGVAYHAERLGIPATIVMPSFT
                     PGVKVARTRGFGARVILHGDSLEAAYQHARTLAEAQNLVFVHPYDDLAIMAGQGTLAL
                     EMLAAQPELDTLVVPVGGGGLISGIATAAKALRPGIRVIGVQTQRYPAMVNAVQGTRH
                     AQGGNTIAEGIAVGAPGAHCLEVIRQRVDDLLLVDEGDIEQAIVLLLEIEKTLAEGAG
                     AAALAALLRHGERFAGRKVGMVLSGGNIDPQLLAQIIGRGLVRSGRLIRIVISARDVP
                     GSLARITALVAEAGANVDEVHHQRAFTLLAAQNVEIEMVLQTRGHEHVQELLQRLRGA
                     GLEVRLATA"
     gene            19282..19431
                     /locus_tag="PKN51_01830"
     CDS             19282..19431
                     /locus_tag="PKN51_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83868.1"
                     /translation="MPPPPSAPQAPHRHPPLLLRSAGWRLGVAAALGALLLLLWRWAL
                     AGASH"
     gene            19447..20235
                     /gene="aztA"
                     /locus_tag="PKN51_01835"
     CDS             19447..20235
                     /gene="aztA"
                     /locus_tag="PKN51_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HNT83869.1"
                     /translation="MSAPILLHDLTLGYDGHPAVHHVSGVIEAGALLAVVGPNGAGKS
                     TLIKGLAGLLKPLGGRIEGLAGQRVAYLPQQATLERGFPVTVGEFAAMGLWRECGAFG
                     GFSPAQRQRVREALAQVGLEGHARQPIDTLSGGQLQRTLFARLMLQDADVLLLDEPFA
                     AIDQRTTDELLALLRQWHAGGKTVLAVLHDLHQVRASFPRTLMLARELRAWGDTADVL
                     TPANLQGLRVMPEAFDEHAPVCEAAPGHDHSHHGRAHHHAEHDR"
     gene            20301..21128
                     /locus_tag="PKN51_01840"
     CDS             20301..21128
                     /locus_tag="PKN51_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNT83870.1"
                     /translation="MRRALVGCLALSLGCAPVGVFLLLRRMSLTGDAMAHAILPGAAI
                     GYLLSGLSLTAMSLGGVAAGLLVAVGSGLVARSTVLREDASLAAFYLLSLALGVLIVS
                     TRGSSVDLLHVLFGSVLALDDAALGLLVGIALLTLATLALLYRPLVLECLDAQFLRSV
                     SRFSPVAHYGFLVLVVINLVAGFHALGTLMAVGIMVLPAATARLWVRGVPALMALAAG
                     LALAACVVGLLLSYHLDWPTSPTIVLCLGGVYLLSLVAGRFGAWRQGQHGRQRHLRG"
     gene            21223..22140
                     /locus_tag="PKN51_01845"
     CDS             21223..22140
                     /locus_tag="PKN51_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNT83871.1"
                     /translation="MRRHLVRATLALLTALPALAQGAAPLRVVASFSLLADMVRQVGG
                     DRVTVSALVGPGADAHVFQPTPAQASQVGQAQLVVTNGLGYEGWLPRLLRSAGYRGPQ
                     VVAARGIEPLPATPGHGHGGHGRDAHGHTDPHAWQSVPHAMVYVKNIADGLCAADPAG
                     CADYRAGAARYSAELKALDAEIRAAWAPIPAPRRLVITAHAAYAYYGAAYGVRFLAPQ
                     GVSTDSEASARGVAQLVRQIRQQGIRALFIENIADPRLIEQIARETGVRPAGRLYADA
                     LTEADGPAPSYVALMRANTRALTQAVGGP"
     regulatory      22154..22252
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            22276..25068
                     /gene="metH"
                     /locus_tag="PKN51_01850"
     CDS             22276..25068
                     /gene="metH"
                     /locus_tag="PKN51_01850"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNT83872.1"
                     /translation="MSAAAPPPPPPMRLSGLEPLTLGEGTLFVNVGERTNVTGSKAFA
                     RLILNGEYEQALAVARQQVENGAQIVDVNMDEAMLDSQAAMVRFLNLMASEPDIARVP
                     VMIDSSKWSVIEAGLRCLQGKGIVNSISMKEGVEEFRRQARLIRRYGAAAVVMAFDEQ
                     GQADTYQRKIEICERAYRILVDEVGFAPEDIIFDPNIFAIATGIEEHAGYAVDFIEAT
                     RWIKQHLPGAKVSGGVSNVSFSFRGNDPVREAIHTVFLYHAIRAGMDMGIVNAGMIGV
                     YDELDPELRQRVEDVVLNRRPDAAERLLDIAEAAKGAAKDDSKKLEWRQAPVRERLTH
                     ALVHGITDFITEDTEQAYRQILQDGGRPLHVIEGPLMDGMNVVGDLFGAGKMFLPQVV
                     KSARVMKQAVAHLLPYIEAEKQALAAAGGDVRSKGKIVVATVKGDVHDIGKNIVTVVL
                     QCNNYEVVNMGVMVPAHEILAKAREEGADIVGLSGLITPSLEEMQHVAGEMQKDAYFR
                     ERQIPLLIGGATTSRVHTAVKIAPHYDGPVIYVPDASRSVGVAQGLLGADVARYWADV
                     RADYERVRQLHAGKKQAPLWPIERARANKARIDWGNYTPPVPKFIGRRVFQHIELAEL
                     LPFIDWTPFFQTWDLAGKYPAILNDEVVGQEARRVFQDAQTMLKKIVDGRWLTANAVV
                     GFWPANSVHDDDIELYADEARQHVVLTWHGLRQQSEKQEDAAGQLRPSRCLADFVAPR
                     NAIDSVADGACQSGSGGQNAFKDYLGAFAVTAGIGVEDKVAEFMAAHDDYSAITLKAL
                     ADRLAEACAEWLHHRVRTDLWGYAADEGLSNEELIAERYRGIRPAPGYPACPDHSVKR
                     PLFELLRTQEIGMGLTESYAMTPPASVSGFYFSHPQSSYFNVGRIDMTQVADMAARRG
                     MAPAALERLLAPNLGL"
     gene            complement(25084..25887)
                     /locus_tag="PKN51_01855"
     CDS             complement(25084..25887)
                     /locus_tag="PKN51_01855"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNT83873.1"
                     /translation="MKVRVLGCSGAIARDCRTTSFLVDQDILIDAGTGVGELTRDEML
                     RIEHVFLTHSHLDHVACLPLMVDTMACDRIGRPIHVHALPQTIAALRAHVLNDVIWPD
                     FSRLPTPELPLIQFHPLQVGHTRELGGRVIEALPARHAVPALGFALRRQATDAPWWVF
                     SGDTSGHPDFWQRLNQLRVHSLVIETAFANRERELAQMAQHLSPETLAEELDRIDRRH
                     RFPIYITHTKPADTETIMREIAQFDQVRPPRREVAHDIRWLSAGHVFEL"
     gene            complement(25905..28109)
                     /locus_tag="PKN51_01860"
     CDS             complement(25905..28109)
                     /locus_tag="PKN51_01860"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT83874.1"
                     /translation="MLASALLVLLASLHAVGVWRAPLLDRLDEVLYDLRLRLTMPGTL
                     DERVVIIDVDEPSLARHGQWPWRRAEVARLIEELIERQQVAALGLDVVFAELDAGGGL
                     GALERLARGELRDNPAFLDWLARTAPTLDGDRLLAARLARAPVALGYYFSSDRAGSRT
                     GALPEPWIRPDRLPRGLLHWDGYGGNIAVLNGAARTAGFFNAVADPDGSVRAVPLVAG
                     FEGALYPSLSLATLRLGLGARQLREERLGPEADAPLQALWLGDDVHRWRLPVDDRGTV
                     LVPYRGPGGPQGGSFRYISASDVLQGRLAPAELKGRYALLGFTAPGLMDLRATPVAEV
                     YPGVEIHANLISGMLDGRVAVRPDYAAGYEVGLLVLLGGVLALGLPLLSVGGALGLGA
                     ALLAALLALDLSLYVGAGLALPLATPLALILIALTLDMALGYFVEGRAKRALARQFAT
                     YVPPELVQQMLQQPERYGMAARSQELTVMFCDLHGFTSLAETMEPQTLQTLLGEVLSH
                     LTEVIQTHQGTIDKYIGDCVMAFWGAPVAMPDHARRAVDAAVAIVASLRDLNADHAAR
                     GLPAVSVGIGLNTGVMSVGNMGSDLRRAYTVIGDAVNLAARLEALSRVYEVDLVASQA
                     TRDAAAAAGHVWQELDRVRVKGRHQAVSIHTVRAAAGQDDVALQEELAAWRQALSLWR
                     AGHFAEFGSAVEALQARNANYYLYRLYAERLVFLLRFPPGPDWDGTTVFDVK"
     gene            complement(28235..28897)
                     /locus_tag="PKN51_01865"
     CDS             complement(28235..28897)
                     /locus_tag="PKN51_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNT83875.1"
                     /translation="MPKLIVSIGGVVVKEVQLTKDRTTIGRRPYNDVVIDNLAVSGEH
                     AVLQMGAGQVTIEDLGSTNGTYVNGRSVKSEVLESEDSIEIGKYKIRFLDGEASAPSP
                     EPVGIHSDSATAIGLSLPMGIDAPPAPSAEQLVGPRVRVLSGLAAGRELPLTKVVTTI
                     GKPGICVASITRKSHGFDLAQVEGQQMARVNGVTVDVGPITLVDRDQMELGGVRLEFL
                     ER"
     gene            complement(28982..29767)
                     /locus_tag="PKN51_01870"
     CDS             complement(28982..29767)
                     /locus_tag="PKN51_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNT83876.1"
                     /translation="MEFFYSALTDVGRVRANNEDAVRVDAEHGIVVLADGMGGYNAGE
                     VASAMAVDLIVEELGRWLHEAAAAATLRDVRRAMEICVDNANRAIFEAANTNDDCAGM
                     GTTLVMGAVLGSTLLVGHVGDSRAYRWRAGVLTLLTRDHSLLQEQIDIGLITPADAAA
                     SGYRNLVTRALGVEDTVLLDVQEVGIASGDVLLLCSDGLNDMLHDADIAAVLGSAESP
                     HEMSQHLIELANGRGGRDNVSVIVVKAQEPPRKPGMMAKLLRK"
     gene            complement(29835..30542)
                     /locus_tag="PKN51_01875"
     CDS             complement(29835..30542)
                     /locus_tag="PKN51_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNT83877.1"
                     /translation="MEDFLTAHFWLAVGQIIMIDILLGGDNAVVIALACRGLPPHQRT
                     KGIIWGTVGAIVLRVILIAFALALLQIPFLKLVGALLLIWIGVKLIAPEEEDGHDGIQ
                     SSDKLWGAVKTVIVADLVMSVDNVIAIAGAAQGAGEQHQLALVIFGLLVSIPIIVWGS
                     QLVLKLMDRFPVIILLGGMLLGWIAGTMAQSDPALATFIPQDRAWHYGMGVVGALLVL
                     ALGKFLERRRASASAVT"
     gene            complement(30647..31540)
                     /gene="sucD"
                     /locus_tag="PKN51_01880"
     CDS             complement(30647..31540)
                     /gene="sucD"
                     /locus_tag="PKN51_01880"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNT83878.1"
                     /translation="MSIYINKHTKVITQGITGKTGQFHTEKCQEYANGKNCFVAGVNP
                     KKAGESIFNIPIFATVKDAARETGATCSVIYVPPAGAAAAIWEAVEADLDFVVCITEG
                     IPIRDMLEVRNRMKEKEARGGKKTLLLGPNCPGVITPDEIKIGIMPGHIHKKGRVGVV
                     SRSGTLTYEAVAQLTELGIGQSSAVGIGGDPINGLKHIDVMKAFNDDPDTDAVIMIGE
                     IGGPDEAEAARWCKDHMKKPIVGFIAGVTAPPGKRMGHAGALISGGADTADAKLAIME
                     ECGFKITRNPSEMGRLLKALL"
     gene            complement(31554..32717)
                     /gene="sucC"
                     /locus_tag="PKN51_01885"
     CDS             complement(31554..32717)
                     /gene="sucC"
                     /locus_tag="PKN51_01885"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNT83879.1"
                     /translation="MKIHEYQGKEILRQFGVPVPRGIPAFTVQEAVEAAQKLGGPVWV
                     VKAQIHAGGRGKGGGVKVAKSLEQVKELAGQILGMQLKTHQTGPEGQKVRRLYIEDGA
                     DIQKEYYLSVVTDRASQKVAFIASSEGGMDIEEVAHSTPEKIVKVFVDPLVGFTDAQG
                     QELARGVGMPADSAAQFVDVCRKLYQCYMDTDASLVEINPLNRDSKGNIIALDAKFNF
                     DSNALFRHPEIVALRDLDEEDPAEIEASKFDLAYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGAEPANFLDVGGGATPEKVTEAFKIMLRNPKVKAILVNIFGGIMKCDTIATGVIT
                     ASRAVDLKVPLVVRMKGTNEELGKKMLAESGLPIISADTMAEAAQKVVAAVQA"
     gene            32915..33829
                     /gene="argC"
                     /locus_tag="PKN51_01890"
     CDS             32915..33829
                     /gene="argC"
                     /locus_tag="PKN51_01890"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNT83880.1"
                     /translation="MAKVFIDGEAGTTGLQIRERLQGMAGVELLSIDPARRKDPQAKR
                     DLMAAVDLVVLCLHDDAARESVAMIDALGERRPRIIDASTAHRVAPGWVYGFPELSAQ
                     QRAAVQAAERVANPGCYATGAIALLRPLVDAGLLPPEHPVALPSVSGYTGGGRQMIEA
                     YEAGNAPPYELYALGLKHKHIPEIMRYGRVTRRPIFVPAVGNFDQGMLVELPLHLDLL
                     PGQPSAEQLLRTYRAHYAGAEAVSVEAAPESGKLDAVALKGTNQLEIRVFANPEAAQA
                     VCVARLDNLGKGASGAAVQNLKLMLGLD"
     gene            complement(34115..34594)
                     /gene="recX"
                     /locus_tag="PKN51_01895"
     CDS             complement(34115..34594)
                     /gene="recX"
                     /locus_tag="PKN51_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HNT83881.1"
                     /translation="MARAPLSLKARALRYLAAREHSRAELATKLARHATEHESPAAIE
                     RVLDELAAKGFIDEARVAESVLHRRAPRLGSARVLHELRTKGLPEAVVADAARQLQGS
                     EFDRALAVWRRRFAAPPGDAGERARQLRFLAGRGFSAEVAWRVLSAAAQATGPSNGG"
     gene            complement(34666..35712)
                     /gene="recA"
                     /locus_tag="PKN51_01900"
     CDS             complement(34666..35712)
                     /gene="recA"
                     /locus_tag="PKN51_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNT83882.1"
                     /translation="MDMAVADKNEKAKALQAALAQIEKQFGKGTIMRLGEGEAIEDIQ
                     VVSTGSLGLDVALGVGGLPRGRVIEIYGPESSGKTTLTLQVIAEMQKLGGTCAFIDAE
                     HALDTQYAQKLGVNLSDMLISQPDTGEQALEIVDSLVRSGAIDLVVVDSVAALVPKAE
                     IEGEMGDSLPGLQARLMSQALRKLTATIKKTNCTVIFINQIRMKIGVMFGSPETTTGG
                     NALKFYASVRLDIRRIGTIKKGDEAIGNETRVKVVKNKVSPPFKQAEFDILFGEGISR
                     EGEIIDMGVTAKVLDKSGAWYAYNGEKIGQGRDNAREFLRENAELAREIENKVRESLG
                     VAQRPAALADADEV"
     gene            35854..36327
                     /locus_tag="PKN51_01905"
     CDS             35854..36327
                     /locus_tag="PKN51_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT83883.1"
                     /translation="MSPDAPEDRWRQTHLGRLLGHAMRRFDARVLQLMAASERAPLAL
                     SNLAARDKVGAAIVHVTRHLPLTGARLTELAAWAGMSKQAMGDLVDQCVAWGLVTREG
                     DPLDARARRIAFTPAGLDWLAAFRDAVAQAEAEFRAAVGTEVATVVALGLEAYAA"
     gene            36464..37138
                     /locus_tag="PKN51_01910"
     CDS             36464..37138
                     /locus_tag="PKN51_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT83884.1"
                     /translation="MRILIAEDDQVLADGLMRGLRNSGAAVDHVASGSEADAALMTNA
                     EFDLLILDLGLPKMNGLEVLRRLRARGQTLPVLILTAADGVEERVKGLDYGADDYMAK
                     PFALSELEARVRALTRRGMGAASAIIRHGPLEYDQTGRVASIDGKMIELSARELGLLE
                     VLLQRSGRLVSKEQLVELLCEWGEEVSNNAIEVYIHRLRKKIEKGPIRIATVRGLGYC
                     LEKVSA"
     gene            37196..38647
                     /locus_tag="PKN51_01915"
     CDS             37196..38647
                     /locus_tag="PKN51_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HNT83885.1"
                     /translation="MKLFQRNQRSLFGEILDWMLTPLLLLWPISLALTWLVAQNLANK
                     PFDRALVYNVQALAQFIQVGPRQRARFSLPQPASELLRADDADNVYFQVMGAHGEFLA
                     GERELPAPPAPGLSEDDAALSDMPQLRDAEFRGLPVRVAYLWVPIAGAPQPALVQVAE
                     TREKRSVLATEIIKGVMLPQFAILPLAVLLVWLALVRGIRPLSELESRIRARRPDDLS
                     PLDDHAVPQEVAPLVASVNDLLTRLKDSIATQKRFLADAAHQLKTPLAGLRMQAELAQ
                     REGASEEELKQSLQQIGLASVRATHTVNQLLALARAEGGSAALAHEPCDLAALTIEVV
                     RQAVPTALEHGTDLGYEGPEPGAPHLVRMGNPVLLKEMLRNLVENALHYARTPGTGAA
                     VVTVRLRANPPGGGLALEVEDNGPGVPAAERELVFQPFYRVLGTNVDGTGLGLPIVRE
                     IAEQHGASVSLSEARPGQQPPGARFVVRFMAPA"
     gene            complement(38751..40160)
                     /gene="mnmE"
                     /locus_tag="PKN51_01920"
     CDS             complement(38751..40160)
                     /gene="mnmE"
                     /locus_tag="PKN51_01920"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNT83886.1"
                     /translation="MPAAAPDPIVAIATAPGRGAVGIVRVSGAGLGPLAQALTGRVLT
                     PRHASYGPFTEADGSPIDHGLALFFPAPHSYTGEDVLELQAHGGPVVLQLLLARCLQA
                     AAEPDPVRGAPRLPHLRLARPGEFTERAFLNDKLDLAQAEAVMDLIDASTAAAARSAS
                     RSLAGEFSRQIRQLVDGLIQLRLLIEATLDFPEEEIDVLAAHDVPGQLSKLQQTLTQV
                     RARARQGALLREGIQVVIAGQPNAGKSSLLNALAGAELAIVTPIAGTTRDAVRQTIQI
                     DGVPLHVLDTAGLREAGDEVERIGIERAWGHIEQADAVLWLHDLTRQGAPDYAAAEAQ
                     IARGLADRLPAGVPVLHLWNKHDAAPHAAIAPGDIALSARTGEGLDALRRRLLELVGW
                     QGGAGEGLFIARQRHLQALARAHEHLQTAQAQLDGSAALDLLAEELRLAQQALGEITG
                     AFSADDLLGAIFARFCIGK"
     gene            40206..41489
                     /locus_tag="PKN51_01925"
     CDS             40206..41489
                     /locus_tag="PKN51_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT83887.1"
                     /translation="MDRLLIQNVHVPDLRRGTRRAHHQVLVEGGVIRAVTDEALPAAG
                     ARVLDGGGRTLLPGLIDCHAHVTFSQTNLALLADMPQTTLVLRALPILRGMLGRGFTT
                     VRDAGGADWALKQAIDDGLDGSVLGPRLFVSGPALSPTGGHGDFRPRSDRLRPLGCGC
                     QRLASTRVVDGVDGVRRAVRELLQMGADQIKIMSSGGVASPTDPIDALGYSVEETRAI
                     VDEAAARHTYVLAHAYTPAAIRRAVDCGVRSIEHGNLIDAPTADRMAERGAWAVPTLI
                     TYEALAQGGVRLGLGADSAAKIERVRAAGLRSLAIFKAAGVKMALGTDLLGGSHGLQS
                     EELLIRAQVQSNLEVLQSATLIGAELLGQAGQLGEVSPGARADLLLVDGDPLQDLRCL
                     TGQGEGIALVMQAGRIVIDRRSGPTRPPTEGQSGL"
     gene            complement(41604..43190)
                     /locus_tag="PKN51_01930"
     CDS             complement(41604..43190)
                     /locus_tag="PKN51_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT83888.1"
                     /translation="MARPLFVRGAVALAALTLAGAAQAAALRIAGANDILTFDPHAQN
                     HQTTLAYQQMVYESLVRYTEKYEVEPSLATKWTFVTPTQLRFELRKGVKFHDGAPLTA
                     DDVVFSLNRAMTPPSNMTAYVQSVKEVKKVDDHTVDVILKAPSPVVLRELTDARIMNK
                     AWAEKNNATKAQDYKAKEENFAARNANGTGPFILEAWQPDTKTTLKKNDAWWDKPKGN
                     VDSIVFTPIKAAATRSAALISGQVDMVVDPPVQDLERIKRDPNISVIEGAENRTIFIG
                     FDQFRDELLGSNVKGKNPLKDKRVRQALYQAVDIAAIERNVMRGLGKPTGTMIAPMVN
                     GWTKELATRAAKFDVAAAKKLLADAGYPDGFELTLDCPNDRYLNDEAICQAITSMWTK
                     IGVKTKLQSNPMAIHSAKIQRFEVSAYLLGWGVATFDALYSLDSLISTVDTKGGAAGN
                     FNCGRMSDPKVDGLIQQIKSEMEAKKRTEEIHQALQIVKDNYNYLPLHDQIRPWAMRK
                     GVTTVHRADDRPMAIWTTVK"
     gene            complement(43425..45113)
                     /gene="yidC"
                     /locus_tag="PKN51_01935"
     CDS             complement(43425..45113)
                     /gene="yidC"
                     /locus_tag="PKN51_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNT83889.1"
                     /translation="MKDIRRTILWVIFAFSLVMLWDQWQVHNGRKATFFPGAPTPASQ
                     ASAPASAVPAASAQPAAVPGVAPAGVPPGDAAPAAAAAAPRERIEVTTDVLKLVLDTE
                     GGSIVRSEFLKFDQTEHPGQAFVLLDDSPQRTYQAQSGLIGGPFPTHKTAMRFTGERT
                     LAEGASELVLRFESPELGGLKLVKTYTLRRGDYVAQVRHEVVNTGGQPVSPQLYVQLV
                     RDGNPAPGGTSFYSTFTGPAFYTDAKKFQKVEFADIDKGKAEFATPSPDGWVAMVQHY
                     FASAWLLPQGMGHDNFARKLDGGLYAAGMIASLGAIAPGASQAVQARLFIGPQDEKVL
                     EGIAPGLELVKDYGYFTILAKPLYWLLDKLHGVLGNWGWAIVALVVLLKIAFYWLNAK
                     AYASMAKMKAINPRIQELRERLKDNPQQMQMEMMRIYREEKVNPMGGCLPIMIQIPVF
                     IALYWVLLSSVEMRGAPWILWIKDLSVKDPYFILPVVMTLSTLLQTALNPLPPDPMQA
                     RMMWIMPLVFSVMFFFFPAGLVLYWVTNNILTIAQQWIINTRMGVPPQFNLPKF"
     gene            complement(45110..45397)
                     /gene="yidD"
                     /locus_tag="PKN51_01940"
     CDS             complement(45110..45397)
                     /gene="yidD"
                     /locus_tag="PKN51_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNT83890.1"
                     /translation="MMRALLIGLVKAYRLLLSPWLGSSCRFEPTCSLYAIGALQRHGA
                     LAGSYLTLRRLLRCHPGCDGGCDPVPPQPPRFFTRLIDRERSDPSTPRQPS"
     gene            complement(45394..45804)
                     /locus_tag="PKN51_01945"
     CDS             complement(45394..45804)
                     /locus_tag="PKN51_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNT83891.1"
                     /translation="MRSATPGRLRTREQFQALLAQRPVARTAHFALHRLAGPQGLPDA
                     VAPGLFCPAGVAWLGAMAPKRWARRAVTRNLIRRQIYAVGERLQPPLAAGAHLVRLRA
                     AFAPAQYPSAASVALQRAVRAELCQLFAQGRSAS"
     gene            complement(45863..45997)
                     /gene="rpmH"
                     /locus_tag="PKN51_01950"
     CDS             complement(45863..45997)
                     /gene="rpmH"
                     /locus_tag="PKN51_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNT83892.1"
                     /translation="MKRTYQPSKTRRARTHGFLVRMKTRGGRAVINARRAKGRKRLAV
                     "
     gene            46399..47826
                     /gene="dnaA"
                     /locus_tag="PKN51_01955"
     CDS             46399..47826
                     /gene="dnaA"
                     /locus_tag="PKN51_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNT83893.1"
                     /translation="MHIDQPQRASASHGAALWDTCLETLARELPEQQFNTWIKPLSAQ
                     LAADHSRLTLYVGNRFKLDWVRAQYATRIAAVLEALQGHAVQVELALAPRAAPIKPSF
                     SIPTSETTQGDPEPARAPEPLPAPTGDRDSAGPVRTRLNGALTFDTLVEGTANRMARA
                     AALHVAGAPGQLYNPLFIYGGVGLGKTHLMHAVGNRLLQTRPDAKVLYIHAEQFVSDV
                     VKAYQRKTFDQFKERYHSLDLLLIDDVQFFANKDRTQEEFFNAFEALLAKKSHIVMTS
                     DTYPKGLTDIHERLVSRFDSGLTVALEPPELEMRVAILISKAAAEGAEMPEEVAFFVA
                     KNVRSNVRELEGALRKILAYSRFNQKDISIALAKEALRDLLSIQNRQISVENIQKTVA
                     DYYKIKVADMYSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVR
                     KIAGERQQLTELNQQLHVLEQTLKG"
     gene            47945..49075
                     /gene="dnaN"
                     /locus_tag="PKN51_01960"
     CDS             47945..49075
                     /gene="dnaN"
                     /locus_tag="PKN51_01960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNT83894.1"
                     /translation="MIVLKTTQDRLLAALQSVAGIVERRHTLPVLANVLLRKSGDTLQ
                     LTTSDLEIQIRTAAELGGDSGHFATTVGARKLIDILRTMPADQTVSLESSQSKLILKG
                     GKSKFTLQTLPAEDFPLVQEAPSFGASFQVPQKTLKTLLDQVAFAMAVHDIRYYLNGI
                     LFVAEGKQLSLVATDGHRLAYASATLEADVPKQEVILPRKTVIELQRLLSDASVPEGQ
                     QAPMIEMQFAANQAKFSFGAMEFVTKLVEGKFPDYNRVIPRNHRNSVTLGRAPLLASL
                     QRTAILTSEKFKGVRLNFEPGSLQIASNNSEQEEAVDEIDIDYDGEPIEIGFNVTYLI
                     DALANMNQQEMVRVELQDGNSSALITLPDNAHFKYVVMPMRI"
     gene            49160..>49589
                     /locus_tag="PKN51_01965"
     CDS             49160..>49589
                     /locus_tag="PKN51_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT83895.1"
                     /translation="MTDPRPDPDQPAAATPSAGAEGYGEGAIQILEGLEAVRKRPGMY
                     IGDTSDGTGLHHLVFEVVDNSIDEALAGHCDDIVVTIHSDGSLSVVDNGRGIPTGVKM
                     DDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVS"
BASE COUNT         7873 a        17171 c        16901 g         7544 t
ORIGIN      
        1 ccgccgcggc ccttgccctt gacggccagg ccggcggcca gcagggcttc gcgcgcggcc
       61 tgaaagtcgt cggcggtgac cgggccgggg tgcccggcgg cctgcgcggc ggccagaaac
      121 tgctgctgca gcgtggcgtt gcccacggtg ctgtggtcgg cgggcagcag gttgagcagc
      181 agtgcttcca gcgcttgctg gcgttgcatg gacatggact ccgtcccctc ttggtatgga
      241 ttgcagtaag ttatagcagg cgggccgagg ggaatacggc cgtgcaaaca agcgttgcga
      301 cgcgctttct acaatcagct catgaccgcc cccgcctaca cccgcgccgc cgcgctgccg
      361 gccatcctgc gccaacgcct cgtgatcctg gacggggcca tgggcaccat gatccagcag
      421 ctcaagttga gcgaggcgca gtttcgcggc gagcgctttg ccgatttcgc caaggacgtg
      481 aagggcaaca acgagctgct gagcctgacg cggcccgacg tgatccaggc gatccacgag
      541 aaatacctgg ccgccggcgc cgatctggtt gagaccaaca ccttcggcgc caccaccatc
      601 gcccaggccg actacggcat ggaagcactg gcgcgcgaga tgaacgtggc ctcggcccgg
      661 ctggcgcgcg ccgcctgcga caaatacagc acacccgaca agccgcgctt tgtggccggc
      721 gccctgggcc cgacgccgcg caccgccagc atcagccccg acgtcaacga ccctggcgcg
      781 cgcaacatca gcttcgagga gctgcgcgcc gcctaccggg agcaggccga agggttgatc
      841 gagggcggcg ccgacgtgct gctggtggaa accatcttcg acaccctgaa cgccaaggcg
      901 gcgctgtttg ccatcgacga ggcgttcgag cacagcggcg agtccctgcc gatcatcatc
      961 agcggcaccg tgaccgacgc ctcgggccgc gtgctgtcgg gccagaccgt caccgccttc
     1021 tggtacagcg tgcgccacgc ccggccgctg gccgtgggcc tgaactgcgc gctgggcgcg
     1081 gcgctgatgc gcccctacat ccaggagctg gccagggccg cgcccgacac cttcatcagc
     1141 tgctacccca acgccggcct gcccaacccg atgagcgaca ccggcttcga cgaaacgccc
     1201 gaggtcacca gccgcctgct gcacgaactc gccgccgagg gcctggtcaa catcgtcggc
     1261 ggctgctgcg gcaccacgcc cgagcacatc ggcgccatcg cccgcgccgt ggggccgctg
     1321 gcgccgcgcg gggtgcaggc ggcgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnngcgc catcgcccgc gccgtggggc cgctggcgcc gcgcggggtg caggcggcgg
     1501 ggttttacaa ggaagcggcc tgaagggcgg gcgccccggt gcctggggct cgctgaattt
     1561 tttgtgaaac agggcgtcaa cccttgctgg atattcccga tcagctcttg attttgtagc
     1621 tatcggttgg gccgcagcaa caccgtgccg atggccacca ccacgcagac catcgccgcc
     1681 aggtcgtgcc aaccaggcca ttcgcccacg atcagcgggg cgctcagggt gccgaccagc
     1741 ggcaccgcca tcacgctcat ggcgctggtg gccgcaggca gggcgcgcgc cagcgcaaac
     1801 cagatcacct gcgccgcgcc gtaattgatc agcaccgacc acagcaggct gagccagacc
     1861 gcggcgctca aggatgcccg ctgcggcggc tccagccacc atgccagcag cgccagcacc
     1921 agcgccgacc aggccatcat ccacaccacc agcgactcgg ccggcagggt cagatgcgcg
     1981 cgccgcatcc agatggtgcc caaggcccag gccagcgctg cgccctgcat ccaggcgatg
     2041 cccgccgggc ggccggccaa ctgggccagc tcgccccaca gcagcagggt gatgcccgcc
     2101 agcacggcgc ccaccgccag cagcagacgc gcgctgaggc gctcgccgta gaacagcacc
     2161 gacagcagca ccgtccacac cggcatggtg aagcccagga tggcggcccg accggcgggc
     2221 agatgggcca cgccgatgat cgacagcgcg tgccagccca gaatgttggg cagacccagc
     2281 accaggacgt cgcgccactc ggcggtgccg cgcggccaca ggcgcaggcc acgggcggca
     2341 aagtaggccg ccagcaccag cgcacccagg cccatggtga gggcacggaa gtgcagcggc
     2401 gccagctcgc gcagcgacag cttcatgatg ggccagttca ggccccacat cagggtcagc
     2461 agcagcaggc cggcgagctg gcggcgcgtg agggtggtca tcggcgccag cttaccgagc
     2521 ccagcaggca gcccgggttc acaccgatgg cgcagagtct gccgcgagca gggcgcgatc
     2581 gtgcgcggca agatgtgcct cgccatgctc gaggatgaaa tagcgcagcg cctcggctgc
     2641 cggcgacagc agcttggact gcatgtgcac cacgttccag gtgcgcacca acggcgtgtg
     2701 ctcgaccggc acccgttcca gcaggccgca gcgcagctcc aggccgatgg tgtgcagcga
     2761 cagaaagctc aggcccatgc cggccatcac cgcctgcttg atggtctcgt tgctcggcat
     2821 ctccatggcg atgcgcggct cgacccggtg gtcctgaaag aagcggtcca tcagtgcgcg
     2881 cgtaccggaa gcggcctcgc gcacgatgag cgggaacctg gccagcgccg ccagcgatgg
     2941 ttggctgaca gccaggatgg gatgtcgcgg cggtgcgaca aagacatgcg ggtgcgcggc
     3001 aaagggctcg gcgcgcgtgg ccatctcctt cggtgggcgg cccatgatcg ccagatccac
     3061 gtcaccttgc tgcagcatcg ccaccagctg ctcgcggttg ctgcccacca gcagcttgag
     3121 ctcgacgccg gggtgttcgt cgcgaaaacg cgtcagcagc tgcggcacga aatacttggc
     3181 cgtgctgacc agtccgatcg acaaggcccc tgattccagc ttgcgaaagc gcgccatcgc
     3241 atcgtcggcg tccttcagcg tcgcgagcaa ccgcttggcg tagaccagaa agtactcacc
     3301 gaccgtggtg agcgacacct tgcgcccctc gcggttgaac agcggcagct cgatcgccga
     3361 ctccagctcc ttgatctgca tcgtcaccgc cggcggcgtc agatgcagcg cctcggccgc
     3421 gcgcacgaag ctcaggtgcc tggccacttc gacaaaaacc ctgagctgtc ggaaggtgac
     3481 ggtcttcatt cagggattct gaatcgaaag ccaaagatat gtgaatttac cataagcatg
     3541 gtgccgccta cattgaaaaa aagacgcatg ccgcagcacc catggaccat cgcattgccc
     3601 cgtcgatcct ttctgccgat ttcgcccgcc tgggcgagga ggtgcgcgat gtcatcgccg
     3661 ccggtgccga ctggatccac ttcgatgtga tggaccacca ctacgtgccc aacctgacca
     3721 tgggcccggc tgtggcgcag gccatcaagc cccattgcaa gcgtgccgac ggcaccccgg
     3781 tgccgctgga cgtgcacctg atggtgcagc cggtcgacgc gctggccgct gcctttgccc
     3841 aggccggcgc cgacctgatc accttccatc ccgacgccag cacgcacgtg gaccgcacct
     3901 tgcagctgat ccgggcggcc ggctgtcagg ccgggctggc gttcaacccg gcggcaccca
     3961 tcgatgtgct ggagtgggtg atcgacaggg tcgatctggt gctggtgatg agcgtcaacc
     4021 ccgggttcgg cgggcagact ttcatcgacg gcacgctgcc caagctgcga cggctgcgtc
     4081 agtggatcga cgccgaacac gaacggtccg gacgcacgat ccggctggaa gtggatggcg
     4141 gcatcaaggc cgacaacatc cgccgcgtgg ccgatgcggg ggccgacacc ttcgtggccg
     4201 gcagcgcgat cttcggccag cccgactacc gcgccgtcat cgaacgcatg cgtgccgagc
     4261 tcgaacgcgc ccgctgaccg cacccgaacc ccaccgacgc gacaccccga ggaagtccgc
     4321 catgccgctg tccgaacgcc agaccctgac ccagtacctg atcgagcagc ggcgccgctt
     4381 tccgggtgcc agcggcgagc tcaacgcgct gctgctggat gtggcgatcg cctgcaaagg
     4441 catcgcacgg gcggtggccc agggcgagct gggtgcggac ccggcaagcg cgaacgcgga
     4501 tgccggcgac gtcaacgtac agggcgaagt gcaaaagcgc ctggacgtga tctccaacca
     4561 gatgttcctg cgtcagaccg agtggtcggg caacctcgcg ggcatggcct ccgaggagat
     4621 ggcgcaaccc tacccgatcc ctgcgcgcta tccgcgcggc aagtatctgc tggtgttcga
     4681 cccgctcgac ggctcgagca acctcgacgt gaacggtgcc gtcggcagca tcttctcgat
     4741 cctgcgcgcg ccgccggagg tggtcggcgc ccaccgcgac ctcaccgaag cggactttct
     4801 gcagcccggc acctgccagg tcgccgccgg ctacgcgctc tacgggccgg tgacgatgct
     4861 cgtgctcagc gtgggcagtg gcgtggtcgg cttcacgctc gacgcccaca tcggcgagtt
     4921 caagctcacc cacccgaaca tcgcggtgcc cgaggacgcc aacgagtttg cgatcaacac
     4981 ctccaacagc cgcttctggg aaccaccggt caagcgctac atcgacgagt gcctggccgg
     5041 caggagcggg ccgcgcggca aggacttcaa catgcgctgg atcgccagca tggtggccga
     5101 ggcgcaccgc atcctgatgc gcggcggtgt cttcatgtac ccgcgcgaca ccaaagaccc
     5161 gagcaagccg ggccgcctgc gcctgctgta cgaggccaat ccgatcggct tcatcatgga
     5221 acaggctggc ggtcgtgcca gcaccggccg ccagaacgtg ctcggcgtgg cgccgagcag
     5281 cctgcatcag cgcatcggcc tggtgttcgg ctccaggaac gaagtcgagc gcatcgaacg
     5341 ctaccaccag gaacccgctc gcaaggcgcc ggcgccgctg ttcgccgagc gcagcctgtt
     5401 tcgcgagtga aggcgtacac gacacaggag agtccatgtc cgaacgaaat cccatcatcg
     5461 ccatcaccgg ctcctcaggg gcaggcacgt cttcggtgac gcgcaccttc gagaacatct
     5521 tccgccgaga gggcgtgacc gccgcggtca tcgaaggcga cagcttccat cgctacgacc
     5581 gcaaggagat gaaggcacgc caggccgagg ccgagcgcaa cggcaacaag catttcagcc
     5641 acttcggccc agagaacaac ctgttctccg aactcgaaca actgtttcgc agctacagcg
     5701 aaaccggcac cggccgccgc cgcaagtacc tgcacgaccc ggtggaagcg gcgccgtacc
     5761 agcaggaacc cggcaccttc acgccctggg aacccctgcc cgaaggcacc gagctgctgt
     5821 tctacgaagg tctgcatggc gccgtggtca cgccggaagt caacgttgcc cgctacccgg
     5881 acctgctgat cggtgtggtg ccggtgatca acctggagtg gatccagaag ctctaccgcg
     5941 acaagaacgt gcgcggctac tcggccgagg cggtgaccga caccatcctg cgccgcatgc
     6001 ccgactacgt caactacatc tgcccgcagt tcgcgcacac gcacgtcaac ttccaacgcg
     6061 tgccggtggt cgacaccagc aaccccttca tcgcacgcga cattccggcc gccgacgaga
     6121 gcatctgcgt cattcgcttc gccaacccga agggcatcga ctttccgtac ctgctcaaca
     6181 tgatccacaa cgcatggatg agccgcgcca acacgatcgt ggtgccgggc ggcaagatgg
     6241 atctggccat gcagctgatc ttcacgccct tcatctggcg catgatggag cggcggcgcc
     6301 acgccatggg aggactgtga tggcggccca tgccaccgaa cgtgtcgtcc gaggcagcgc
     6361 gggctgcagc gacccacgcg ccaacgcgct gcgtgcgctg gccatcgacg ccgtgcagca
     6421 agccagctcc ggacaccccg gcgcaccgat gggcatggcc gagatggccg tggcgctgtg
     6481 ggcccgccat ctgcgacaca gcccggccaa cccgcagtgg cccaaccggg atcggttcgt
     6541 gctgagcaat ggccatgcgt cgatgctgtt gtatgcgctg ctgcacctga gcggctatga
     6601 cctgccggtg gaggagatcc gacgctttcg ccagctgcac agcagaacgc cggggcaccc
     6661 cgaggtcggc atcacgcccg gcgtggaaac caccaccggc ccgctcggcc agggcatcgg
     6721 caacgcggtc ggcatggcgc tggccgagca gatgctcgcc cgcgagttca accggccggg
     6781 acacaccatc gtcgaccacc acacctacgc cttcgtcggc gacggctgcc tgatggaagg
     6841 cgtcagccat gaagcctgtg cgctcgccgg cgtctggggc ctcggcaagc tgatcgcgct
     6901 ctatgacgac aacggcatca gcatcgacgg gcccgtgcac ggctggttcc gtgacgatac
     6961 gccgtcgcgc tttcgcgcct atggctggga ggtgatcggt ccggtcgatg gccacgacat
     7021 cggcgccgtc gagcaggcca tcgcacaggc ccgggcgggc gccgatcgac cgaccctgat
     7081 cgtctgccgc accaccatcg gacaaggctc gccagcgcgc gccggcaccg cccgggcgca
     7141 tggcgagccg ctcggcgcgg aagaggtggt cgccacccgg gcggcgatcg gctggacggc
     7201 ggatgccttc gacgtgccgc ccgcggtgcg tgcgcagtgg gatgcgacga cggcgggccg
     7261 cgcgcacgag gccgcgtggc aggcgaagtt cgacgcctac cgcgacgccc atccggcact
     7321 ggccgccgag ttcgagcgcc gtgtccgggg cgagctgccg gcgacgttcg gcgccacact
     7381 gcacgccgcc atcgagaccc tgggtgccac gaccgacaag gtggcttcgc gcaaggcctc
     7441 gcagcaggtg ctgggcctgc tggcgcccaa ggtgccggag atgttcggtg gcagcgccga
     7501 cctcaccgga tcgaacctga ccgactttcc cggtgccgac cagcgccacc tgagctatgg
     7561 cgtgcgcgag ttcggcatgg ccgccatcat gaacggggtg gcgctgcatg gcggcttcat
     7621 cccctacggc ggcaccttcc tgaccttcag cgactacagc cgcaacgcca tccgcatggc
     7681 ggcgctgatg aagcagcgcg tgatccacgt cttcacgcac gactccatcg gcctgggcga
     7741 ggacggcccc acacaccagg cggtcgagca tgtgccggcg ttgcgactga tcccgcacct
     7801 cgacgtctgg cgcccggccg acaacgtcga gaccgccgtg gcctggggcg cggcgctgca
     7861 acggcgtgac ggccccacgg tgctggcgct gtcgcgccag gggttgccgc agctgacacg
     7921 gcccacccag gccgaagcca tggcgcgcgg cggttacgtg ttcgccgaac cgccgcagga
     7981 gttgcccgct caggcggtga tcatcgccac cggctcggag ctgcagctgg cacggcaggc
     8041 gcagcagctg ttgtgggccg aaggactgcc ggcacgtgtg gtgtcgatgc cgagcaccag
     8101 cgtgttcgac cgccaggacg ccgcgtaccg cgagcatgtc ctgccaagcc agctgccgac
     8161 ggtggcggtg gaagccgcac acccggactt ctggcgcaag tacgtcggcc gtcggggcgg
     8221 cgtgctgggg gtcgcaacgt tcggcgaatc cgcccccgcc gaggatctgt accggcactt
     8281 cggcctgacg gcagagcgcg tcgccgccgc ggtgcgcgcc tgcgtccggg cggcggcacc
     8341 tggcggtggg gcaggctcct gatgcgggcc gtcctgttcg acctcgacgg cacgctgatg
     8401 gagaccgcac cggagttgac cgacgcgttg aacgacacgc tgcgccggca cgacctgccc
     8461 gcggtcgacg aggcgcgcgt gcgcggctgg atcggtgacg gcgcgcgcgc gttgctggcc
     8521 aaggccttcg cgcacgccgg cgcaacgccg gcagcggcga acgccgcctg gcccgggttc
     8581 caccgcgact acagcgcccg ctgcggcacc cgatcgtccc tgtttcccgg tgcgcgcgag
     8641 ctgctcgccc gcctgagcaa ggcgggtatc cggacggcac tgctcaccaa caaggaatcc
     8701 gccttcgcac acaggctgct ggcgcgccac gatctcgcca gcgccttcga cctcatcgtc
     8761 gccggggaca cgctggccgt gaagaaaccc gatccgggcg tggtcatgca cgcgctgcag
     8821 aagctgcaca tcgaacccgg ccaggcgctg ctgctcggcg attcgctcac cgatgtgcgc
     8881 accgcgcgcg cggcaggcat cgccgtgcgg atcgtgcgcc acggttaccc gcagggcgag
     8941 ctggcgggtg ccgacgcgcc cgacgccttc atcgaccatt tcgacgactt cgatcccggc
     9001 gcatgcaggc acgcgcccgc gacctagttt tcaacgtcaa cccaggagtt cccaatggcc
     9061 ctcgtctcac tgcgccaggt gctggatcac gccgccgaac acggctttgg cgtgccggct
     9121 ttcaacgtca acaacctgga gcaaattcag gccatcatgg aagccgccca ggccaccgac
     9181 tcgccggtga tcctgcaggc ttcggccggc gcgcgcaatt acgccggcga agcttacctg
     9241 cgccacatgg tgctggccgc cgtggaaacc catcccgaca tcccggtggt gttgcaccag
     9301 gaccatggcg cctcgcctgc cgtgtgcatg caggcgatcc gttcgggctt cacgagcgtg
     9361 atgatggacg gctcgctgca ggaagatgcc aggacgccgg caagctacgc gtacaacgtc
     9421 gacgtcacac gccgtgtctg cgcgatggcg cacgccgtgg gcgtgtcggt cgagggcgag
     9481 ctcggctgcc tgggctccct tgagaccggc gaagccggcg aggaagacgg cagcggcgcc
     9541 cagggcaagc tgagccacga catgctgctc accgacccgg cgcaggccag agactttgtg
     9601 gcggccaccg gcgtggacgc gctggcgatc gccatcggca ccagccacgg ggcctacaaa
     9661 ttcagccgcc agcccaccgg cgacatgctg gcgatcgacc gcatcgccag catccacgcc
     9721 gccataccgc acacgcacct ggtgatgcat ggctcgtcga gcgtgccgca ggagtggctc
     9781 gccatcatcc gcaagcacgg tggcacgatc aaggagacct acggcgtgcc ggtcgaggag
     9841 atccagcgcg gcattgccca tggggtacgc aagatcaata tcgacaccga catccgcctg
     9901 gcaatgaccg gcgcgatgcg caggctgttc gccgagcaac cggccgaatt cgatccgcgc
     9961 aaggccatgg ccgaggcccg gcgggcggcc aaaggcatct gccaggcacg cttcgaagcg
    10021 ttcggctgcg ccggacacgc cggccgcatg cggccgattc cgctggatgc gatggccctg
    10081 cgctaccgct gaacccgaac ccggccgttg atcgctggcc agacgccgga gcggcatatg
    10141 gggccacacc gcttccctga ccttcaccgg gtgggcggac gcgcgcggac gacccgcgaa
    10201 tcggacaggg agcacactgc gtcatgttgc gcgcattgat ctgggatgtg gacggtacgg
    10261 tggccgaaac cgaggccgat ggccatcgga tcgcgttcaa ccaggccttc gaggcgtgcg
    10321 ggctcgattg gcactgggac ctgccgactt acggccagtt gctggaagtg accggcggcc
    10381 gcgagcgcct gctcgcctac atggacaggc gcgacgatgc gccggccagc cccgcaggcc
    10441 gtgccgccct ggcacgcgcg gtgcacctgc gcaagaacgc gttctatgcc gatctggtgg
    10501 cgcgcggcgg catcccggcc cgtcccggcg tgcgccggct gatggacgaa tgccaacgcc
    10561 aagaggtgcg gctggcgatt gccaccacca ccagcacgag caacgtcgag gccctgtttt
    10621 ccagtctgtt cggcccgacc tggcgcgatc gcttcgctgc catcgtctgc gccgaagacg
    10681 ccccacgcaa gaagcccgac ccgcaggcct acctgctggc gctgcgtcgg ctcggcgtca
    10741 cgccccaaga ggccttcgcc atcgaggact cgcccaacgg cctgcgggcc gcgcgagcgg
    10801 ccggcctgcg ctgcggcatc acttgcagca ggtatttcgc cgacgccggg ttcgaaggag
    10861 cggcatggct gcggcccgac ctcgagaccc caaggcccat gaccttgcaa accctccagg
    10921 cgctggatgg cgccgcagaa agcgaaaccc ggccgcgcgg ccgaccatga acaagaagat
    10981 cccagccgct cgggtgcccg gggcgcctgc ggcgcgcgag gggcctgttc ggcatgaccc
    11041 taacgcgtca gggccaggaa cagttcgggc agccgccgcg gcagctgttc gacacggtcg
    11101 atcaccgtcc agcgctggcc aaagatgcgg ctgacgtagg catcggcccg cgcgtccaga
    11161 ctgatgcagt gcgtgtacag accttgccgg tcgagctcgc gcaccgcctg cgcggcgtcg
    11221 gcgatcaggt actgcggatc gtccacatcg atatcggcgg gttccccgtc ggtcagcacg
    11281 agcagcagcc gcttgtcggc cctgcgtccc gacagcaggt gtccggcgtg gcgcaatgcc
    11341 gcccccatgc gcgtcgacca ggccccctgc gccgccgcca ggcgggcttt gggcgcgtcg
    11401 ccccacgctt cggtgaaccc cttcaggtgc aggtagcgca cctcctgtcg ggtgttggac
    11461 tgaaacccgg cgatggccag ttcgtcgccc aaggccgcga tcgcccagcc cagcagcgcc
    11521 gctgcggctc ggctgagcga caacaccgtt ggcgcgccgc cggcgggggc gccggtgtgc
    11581 ggctgcccga ccgtctgatt gaccgattgc gacagatcca tcagcaacag caccgaaatg
    11641 tcacgcccgt cggtgcgtgt gctggtgtgg atgcgcgggt cgggctgggc gccggcgcgc
    11701 cagtcggcga tggcgcgcag cgccagatcg aggtcgagtt cggtgccctc ttcctggtgg
    11761 cgcagtcgcg tgcgatcctg cggcttgagc agatcgatcc gccgcttgag gtgccgtgcc
    11821 accggcgcgt actgtgcgag cagcgcgtcg acatcgccgg cctggcctgg gggctgcagg
    11881 atgtcgtaca cgctcaccca gtcgggccga tggccgcgag tcaggtgatc ccactcggga
    11941 tacaggcgcg gcggcagacc gccttcatcg gccaccggag cgcggcgccg cggctcggcg
    12001 ccggtgtctt cctcgtcgcc cggttcgatg aaggtccaca gatggcggtt gtcgtcgcgc
    12061 cagtcgacca gcgtgtcttc gaactgcacg cgcgcccgct gatcgctcgc gcgtcgcgtg
    12121 gcggccgacc agtcgagcgc cagcacggcc atctcgcggg tggacgacac gccgtcgcgc
    12181 agggcggcac gaaaacgccc ggcaaaggcg agcagtgtcg catcgcggta cggatgggcc
    12241 ggatcgagca gcgcgcgcga caggcagacc agccggtgac gcaggcacga cacgcgggcc
    12301 gcatcgcaag cgtcctcggc cggcatgggg tgcagcgcca gcagcgccgg ccgcagaccg
    12361 gggtagcggc gcagcaccag atgatcgaca cgcgcatcct cgaagcactc cagcgccagg
    12421 cgctgcagcg ggctcaggtt gtcggccagc tgcggtgtgc tccagcgaag atgccctgcc
    12481 aggtgggcca gcatggcgcg gtagcgctgc agggcggaaa ctccggcgcg atcgtccagc
    12541 acctcgggca ggcgcaggcc gtcgtcctgc aggtgcggca gcaggggctg gccggtcgga
    12601 tggtcgacat gcaccgggta cggcaccagc ggcagcgcca catcccacag cccacgcagg
    12661 cccagtgcga gccgacgctc gacgtcgatc agcagagtgc cgcggcgctc acgctggagc
    12721 agcgcgcggg cgtctgccga ggtcaggctg aaatagtcga tctggcgctg cgggtgcgcg
    12781 ccatgcagac ggatcccttc ctgcacccag cgccgcaagc cctccatcgg caccagggcc
    12841 accagcaccc ccgcctgctg cagaaaggct ggcaggccag ggctcggttc ggtggcgtgg
    12901 cggccatgga tggaaaggct ggtgcgctgc atcagctcga ccgtcaacgc caggtactgc
    12961 gccagcagat gcggcgccgg aagctgccgc gccaccgccg gcaggctgtg cagcagggtc
    13021 acgatggcgg gtccgttggg cgacttctgg aacgccgccg cggccgcgca cagcggcgcc
    13081 agcgaagact cgccacagca acgcgccacc gcgggccagg cggcgagaaa ctgcagcacc
    13141 ggctgggcgc cgcgccccag cttgccgaac cagcgcgcct ggtcgaggta ggccgccatg
    13201 ccagccgggg tgagcgtgcg cagggcctcg tcgatgcagt cgtcgaacac atcgtcgatg
    13261 gcgggaaagc gacagtcgag gcgctcgcgc agtgccgcat ggcgcgacgc cgcggccgcc
    13321 ggcggcggcg gcgccttgcc gtaggcatcg aagcgggccg tctggctcat cgacagtcac
    13381 accgcgatgg cgtgccgggc tcgaccaggc cgggctttgc cgcctggcct accgcccgtg
    13441 atcgagggac cgacccccgc ccggctgcgg ccgccatcag acgtcgcatt ggcgtcgctg
    13501 caggcatgcg atcagctcat tcaaacacgg catcgatcgc gtggtccagc gactcgcgga
    13561 tgtcggtgtc gtcggtgatc gatcgcacca tggccatgcg gcaggccgcg cgtgcatcga
    13621 tgccctgccg gatcagcgtg gcggcataga ccaccaggcg cgtcgaaatg ccctcgtcga
    13681 gaccatggcc cttgagtcga cgcgccgcac cagccacctg caccagcgcg gcagcgacgg
    13741 ctgcgtcgat cccggtttcg gcaaccacga tgcgcgcctc cagcgcagcc tcgggatagt
    13801 cgaagtccag ggcggcaaag cgctgccgcg tcgaggtctt gaggtccttc atcaggctct
    13861 ggtagcccgg gttgtacgac acgacgagct ggaagtcggc gtgcgcgcgc accagttcgc
    13921 ccttcttgtc gagcggcagc gcacgtcggt ggtcggtgag ggcgtggatg acgaccgtgg
    13981 tgtcctggcg cgcctccacc acctcgtcca ggtagcagat ggcaccgtga cgcgcggccg
    14041 tcgtcagcgg cccatcctgc cagcgcgtgc cgtcgggttc gagcaggaag cgccccacga
    14101 ggtcggccgc ggtcatgtcc tcgttgcacg ccaccgtgac cagggggcgt tgcagcttcc
    14161 aggccatgtg ctcgacgaaa cggctcttgc cgcagccggt gggacccttg agcatgaccg
    14221 gcaggcgcgc cgcataggcg gcctcgaaca gctcgatctc accgccctgc ggggcataga
    14281 agggctcggc ggcgacgcgc cagtcagcga gtgggttcat gacacggcgg gacgttggcc
    14341 aggttggacg gcgcctgccg gtcgatcacc gagcgcgatg gtcggcacgc gccgcacacc
    14401 gacggacctg ctggggtgac aggcacgggc gccgccgtgg gttcagcggt gcacgcccgc
    14461 cagcttgtcg cgccagccgg gatacagctt gtcggcgtcg ccggggaagg agtcgaacgc
    14521 gcgcgcgaac tcgcggtgct cgcgcgccca ttcgatcggg tcggccttgg ccttccagca
    14581 ttcgtaggcc tggcgcagac ttcgggcgcc atcggccggc gagtcgatgt gaccatagct
    14641 gccgccgccg gcggtgttga tgacgttgcc gtggcccagg ttttcgaaga agccaggcag
    14701 gcgcagcgcg ttcatgccgc cgctgatgat gggcgtggtt ggcctcatgc ccagccactc
    14761 ctggtgatac gcggggccgg tgtagctgtc gcgctcgatg atgtaggcaa tggcgcggtc
    14821 gtccttctcg ccttccatct tgccgtagcc catggtgccg acgtggatgc cggaggcccc
    14881 ctgcaaccgg ctcatcttgg ccagcacgta ggcggtgtag ccgcgcttgc tctgcgggct
    14941 ggtcaccgcc ccgtggccgg cgcggtggta gtgcaggtac tggttgggga accagcggcg
    15001 cgcggtggtg accatgccgg gcccgcccac gtagccgtcg accaggaacg ccaccttgtc
    15061 ggcgtccggg ccgaaggttt cgaggatgta ctcgccgcgc gcgagcattt cgctgtggtc
    15121 gtcggcggtg atgttggccg agaagatctt cgcttcaccg gtcctgtcca tggcccgctt
    15181 catcgcgtcg cgaaccagcg gcatcacctg cttcatcggc gcgaagacct ggttgccctg
    15241 cggttcgtcg ttcttgatga agtcgccgcc gagccagaac tcgtaggccg cctgggcaaa
    15301 cggttcgggc cgcaggccaa gcttgggctt gatgatcgtg ccggcgatgt agccgccatc
    15361 cttcagcggg cggccaagca cgcgccacag gtcgctgatg tccttgctcg gaccgtcgaa
    15421 caactgcagg gcacgcggcg gcacccagaa gtcgtacatc ttggcgtact cgatgtcgcc
    15481 catgccctgg ttgttgccga tcgccagcgt cagaaacgag acgatcatca tccggccatc
    15541 gatgaggttg cgatcgaaca ggtcgagggg gtaggcgatg cgcatttcct cgctgacctc
    15601 gtcgatcagg tagacgagcg catcgacccc tttggtgaac tcgtcggtgg tcgagacctc
    15661 gacgttggtg ccggtcgatg actcggccgc gaagtgcgcc gccgccgcca ggtagccgtg
    15721 cccggccttc ggccgcatct tgtaggccac caggatgtgt ttgccgccgc ggatcaggtc
    15781 ttcttctcgc aggctcaggt cggcatagcg gttggattgg tccatctggg gctcctgggg
    15841 tgggttcgct gggatgcgag gcactttaga agaccgattg aataagtaca actaaaataa
    15901 tttggtgctt gaattaagta tttattgaat tcaccccgcc cacctggccg aacttgaaca
    15961 cggccgccgg gcgcgaccgc ccgtgccgtg agcggtgcgc ctggtacgac gtgccgccgt
    16021 gtcagaagtc gaccagactg aggccgatcg aaaacaccgt gcgtttgaag ttgtagtcca
    16081 gcaggctgtc gccatagccg tgaaacagcg ccgtgtacag gcgcaggctg gaaaagctgt
    16141 tgccccagcc gtcgcccagc gagcgcgtcc attccagccg gcccgagccg tagcggttga
    16201 agctgccggt gtaggtggcg cgcagcgtgt tcttggcgtc cacgttccag gccaggctga
    16261 ggtcgccgcg cccgatgtgg cgcacgatgt cggggttgtt gtccttggcg ctgtcttccg
    16321 gcaggcgctt ccacagccgc agttgcaggt tcagcgcgtt gccgcgctcg aagcccgcca
    16381 tcaggtaggc gcggttccag ctgcgcgaca gcgggtcgct ctggccgttg gactggtgcg
    16441 ccaggccgat gccgctgtag cgccacagcc agccgccggg cagcttgagc gtggtcgggt
    16501 agacgtagat cagctcgggc atgtggtcgg tgttgcgaaa cggccgcgac aggctgccgt
    16561 tgaacacctg ccagtacgac tgctgggtgt aggcggccca cagcgaatcg cgcaacgtgc
    16621 cgccggcggg ggtgagcagc ccgccagcca gcttggtgcg caccgacagt gccagccgca
    16681 tttcgcgctt gccgtagtcg atcggcgtga tggcgctgtt ggccgggttg ggcgaggtcg
    16741 gcaggcggtt gatgtggtcg ccggcggcga tcgacgccgt cgtcggatga aaggcgcgca
    16801 ggccgaaggt cgggcagctg gagcccgact ccagttccca gaagcgcgac aggtcggaaa
    16861 actggcggtc gcggcagccc tgctccaacc cgacgccgac gatgccggcc gagcccgaca
    16921 ccagggcccc gtcggcgcgc tcgggctgcg tcgtggccag cgcctgggcc agttcgccac
    16981 gcaccgccgc cgcctgcgca ctgggtgccg cattgcgggt gggcgtggcc tcgaccgact
    17041 gcgccttctg tgcgatggct tcgatcagct gccgctgtcc gtgcgcccag ccgtcgaaac
    17101 aggccaggcg ctgcgcgtcg ggctggccca gacaggcctg ccaggcggtg gcggcgtcga
    17161 tgcgttcggc gccggccggc atggcgggcg attgcgcgct ggccatggcg ggcagcgcca
    17221 gggccagaca gaaccacagg gggtgggggc cggaagggtg catgtgaaac cagaacgatg
    17281 ggcagcgcaa aaagaccgac gggccagcac cggcccgcgg gcaccgattg tgcgtgccgc
    17341 ggcggcgcgc ctgcatccag ttgtgtccac ggccccggcg gcgcaggtcg gccgcggttt
    17401 atgaacccaa tccggctccg acgcccgaac catctgcgcc aaaagctatt gttttcatag
    17461 tacatgggca tgcggaccga cgctgcagct accgccagcg gctggttaca gtgacgctcc
    17521 atgacgcctt ctccactgct gtttccgctg cagggcgcgc tgcggcgcgt gctgtacgtc
    17581 ggcctgtacg aggcgctggc gatcgcggtc gtcaccgtgg ccttcgtcgc tggcgaccgc
    17641 agcctgggtt cggccagcgg cgtggcggtg ggcagctcgc tgatcgcggt gctgtggaac
    17701 ctggtgttca acgccgcctt cgaacgctgg gagtcgcgcc agcgcgtgcg cgggcgcagc
    17761 cgggcgcggc ggctggcgca tgccatcggc ttcgagggcg ggctggcgct gtggctggtg
    17821 cccttcatgg cctggtggct ggaggtgggc ctgtggcagg cgctgctgct ggacctgggg
    17881 ttgctgctgt tctttctggc ctacaccttc gtcttcacct gggtgttcga ccgcctgttt
    17941 ggacttccgc gcgccgccgc gtgacaatgc cgcaccatgc ttgaacttgc cgacatccag
    18001 caggccgccg agcgcgtgcg cggtcaggtg ctggacaccc cctgcgtcga atcgcgcacg
    18061 ctgtcgcagc tcaccggcac gcagttgttc ctgaagatgg aaaacctgca gttcaccgcc
    18121 tcgttcaagg agcgcggggc ctgcaacaag ctggcgcagc tgacggacca ggaaaaggca
    18181 cgcggcgtga tcgccatgag cgccggcaac cacgcccagg gggtggccta ccacgccgag
    18241 cggctgggca ttccggccac catcgtcatg cccagcttca cccccggggt gaaggtggcg
    18301 cgcacgcgcg gcttcggcgc ccgggtgatc ctgcacggcg actcgctgga agcggcctac
    18361 cagcacgcca gaaccctggc cgaagcgcag aacctggtgt tcgtccaccc ctacgacgac
    18421 ctggccatca tggccgggca gggcacgctg gcgctggaaa tgctggccgc ccagccggag
    18481 ctggacaccc tggtggtgcc ggtgggcggc ggcggcctga tctccggcat cgccaccgcg
    18541 gccaaggcgc tgcggcccgg catccgggtg atcggcgtgc agacccagcg ctatccggcc
    18601 atggtcaacg cggtgcaggg cacgcgccat gcgcagggcg gcaacaccat cgccgaaggc
    18661 attgccgtgg gcgcgccggg cgcgcactgc ctggaggtga tccgccagcg cgtcgacgac
    18721 ctgctgctgg tggacgaagg cgacatcgag caagccatcg tgctgctgct ggagatcgaa
    18781 aagaccctgg ccgaaggtgc gggcgcggcg gcgctggcgg ccctgctgcg ccacggcgaa
    18841 cgctttgccg gccgcaaggt cggcatggtg ctgtcgggcg gcaacatcga cccgcagctg
    18901 ctggcgcaga tcatcggccg cgggctggtg cgttcggggc ggctgatccg catcgtcatc
    18961 agcgcgcgcg acgtgcccgg ctcgctggcg cgcatcaccg ccctggtggc cgaagccggc
    19021 gccaacgtcg acgaggtgca ccaccagcgc gccttcaccc tgctggcggc gcagaacgtg
    19081 gagatcgaga tggtgctgca gacgcgcggc cacgagcatg tgcaggagct gctgcagcgc
    19141 ctgcgcggcg ccgggttgga ggtgcggctg gccaccgcct gacccggccc ggggcggcgg
    19201 ccggccgatc tgcgctatgc tcgctcgcct tgcggtcgcc ggcccgttgg cagggcggcg
    19261 cccgcacctg tctgccgccc catgccaccg ccgccctccg caccccaggc cccgcaccgc
    19321 cacccgccgc tgctgctgcg ttcggccggc tggcgcctcg gcgtggccgc ggcgctgggc
    19381 gcactgctgc tgctgctgtg gcgctgggcg ctggcgggcg cctcgcactg acggatccga
    19441 gctgagttgt ctgcgccgat cctcctgcac gacctgacgc tgggctatga cggccacccg
    19501 gcggtgcatc acgtgtcggg cgtgatcgag gccggcgccc tgctggccgt ggtggggccc
    19561 aacggcgccg gcaagtccac cctcatcaag ggcctggccg ggctgctcaa gccgctgggc
    19621 gggcgcatcg aggggctggc cggccagcgc gtggcctacc tgccgcagca ggccacgctg
    19681 gagcgcggct ttcccgtcac ggtgggcgaa ttcgcggcca tggggctgtg gcgggaatgc
    19741 ggggcgttcg gcggcttctc gcccgcccag cgccagcgcg tgcgcgaggc gctggcgcag
    19801 gtggggctgg aaggtcacgc acgccagccc atcgacaccc tgtcgggcgg gcagttgcag
    19861 cgcacgctgt ttgcacgcct gatgctgcag gacgccgacg tgctgctgct ggacgagccc
    19921 tttgccgcca tcgaccagcg caccaccgac gagctgctgg cgctgctgcg ccagtggcac
    19981 gccggcggca agacggtgct ggcggtgctg cacgatctgc accaggtgcg cgccagcttc
    20041 ccgcgcacgc tgatgctggc gcgcgagctg cgggcctggg gcgacaccgc ggacgtgctg
    20101 acaccggcca acctgcaggg cctgcgcgtg atgcccgagg ccttcgacga gcacgcgccg
    20161 gtgtgcgagg ccgcccccgg ccacgaccac agccaccatg gccgcgccca ccatcacgcg
    20221 gagcacgacc ggtgagcggc gagctgctgg agggcggcct gtgggcgctg ctggtggcac
    20281 cgctgaccga gttcggcttc atgcgccggg cgctggtggg ctgcctggcg ctgtcgctgg
    20341 gctgcgcgcc ggtgggcgtg ttcctgctgc tgcggcgcat gagcctgacc ggcgacgcca
    20401 tggcgcacgc catcctgccg ggcgcggcca tcggctacct gctgtcgggc ctgtcgctga
    20461 cggcgatgtc gctgggcggg gtggcggccg gcctgctggt ggccgtgggc tcggggctgg
    20521 tggcgcgctc caccgtgctg cgcgaggacg ccagcctggc cgccttctac ctgctgtcgc
    20581 tggcgctggg ggtgctgatc gtgtccacgc gcggctccag cgtcgatctg ctgcacgtgc
    20641 tgttcggcag cgtgctggcg ctggacgacg ccgcgctcgg gctgctggtg ggcatcgccc
    20701 tgctgacgct ggccacgctg gcgctgctgt accggccgct ggtgctggaa tgtctggatg
    20761 cgcagtttct gcgcagcgtc agccgctttt cgcccgtcgc ccactacggc tttctggtgc
    20821 tggtggtgat caacctggtg gccggctttc acgccctggg cacgctgatg gcggtgggca
    20881 tcatggtgct gccggcagcc accgcgcgcc tgtgggtgcg cggcgtgccg gcgctgatgg
    20941 cgctggccgc agggctggcg ctggccgcct gcgtggtcgg gctgctgctg tcctaccacc
    21001 tggactggcc gaccagcccg accatcgtgc tgtgcctggg cggggtgtac ctgctgtcgc
    21061 tggtggcggg acgcttcggc gcctggcgcc agggccagca tgggcggcag cgccatctgc
    21121 gcggctgagc tggccgggcc gcatcgaaat gcatcatcaa atcggcatcc ggcgcacatc
    21181 aggaaagcgc aaacagctat gcaattgata gtacaccgac ggatgcgcag gcaccttgtg
    21241 cgcgccaccc tggccctgct gacggcgctg cccgcgctgg cccagggcgc ggcgccactg
    21301 cgcgtggtgg ccagcttcag tctgctggcc gacatggtgc ggcaggtcgg cggcgaccgc
    21361 gtgacggtga gcgcgctggt cggccccggc gccgacgccc atgtgttcca gcccacgccg
    21421 gcccaggcga gccaggtcgg tcaggcgcag ctggtggtga ccaacggcct gggctacgaa
    21481 ggctggctgc cgcgcctgct gcgcagcgcc ggctaccgcg gcccgcaggt ggtggccgcc
    21541 cgcggcatcg agccgctgcc ggccacgccc ggccacggtc acggcggtca cggccgcgac
    21601 gcgcatggcc acaccgaccc gcacgcctgg caaagcgtgc cgcacgccat ggtctacgtg
    21661 aagaacatcg ccgacggcct gtgcgcggcc gacccggccg gctgcgccga ctaccgtgcc
    21721 ggcgcggcgc gctacagcgc cgagctgaag gcgctggacg ccgagatccg cgccgcctgg
    21781 gcccccatcc cggcaccgcg ccgcctggtc atcaccgcac acgccgccta cgcctactac
    21841 ggcgcggcct atggcgtgcg ctttctggcg ccgcagggcg tgagcaccga cagcgaggcc
    21901 tcggcccggg gtgtggcgca gctggtgcgg cagatccgcc agcagggcat acgggcgctg
    21961 ttcatcgaaa acatcgccga cccgcgactg atcgagcaga tcgcccgtga aaccggcgtg
    22021 cgtcccgccg gccggctgta cgccgacgcc ttgaccgaag ccgacggccc ggcgcccagc
    22081 tacgtcgccc tgatgcgcgc caatacccgc gccctgaccc aggccgtggg cggcccgtaa
    22141 aatcggcccc ctcgccgcca aggagcgttg cagcgggcac cgcgccgggc gcggccggcc
    22201 cgtcaggctt ggtggcggtt gcccgtctga accgcaacga cgctcacctc gaccgacccg
    22261 atccaggtga gtgccatgtc cgctgccgcc ccgccccccc cgccgccgat gcgtctgtcc
    22321 ggcctggagc cgctgaccct cggcgaaggc acgctgttcg tcaacgtcgg cgagcgcacc
    22381 aacgtcaccg gctccaaggc cttcgcgcgc ctgatcctca acggcgagta cgagcaggcg
    22441 ctggcggtgg cgcgccagca ggtcgaaaac ggcgcccaga tcgtcgacgt caacatggac
    22501 gaggccatgc tcgactcgca ggccgccatg gtgcgctttc tcaacctgat ggccagcgag
    22561 cccgacatcg cccgcgtgcc ggtgatgatc gattccagca agtggagcgt gatcgaggcc
    22621 ggcctgcgct gcctgcaggg caagggcatc gtcaattcga tctcgatgaa ggagggcgtc
    22681 gaggaattcc gccgccaggc caggctgatc cgccgctacg gcgccgccgc cgtggtgatg
    22741 gccttcgacg agcagggtca ggccgacacc taccagcgca agatcgagat ctgcgagcgc
    22801 gcctaccgca tcctggtcga cgaagtgggc tttgcgcccg aggacatcat cttcgacccc
    22861 aacatcttcg ccatcgccac cggcatcgaa gagcacgcag gctacgcggt cgacttcatc
    22921 gaggccacgc gctggatcaa gcagcacctg ccgggtgcca aggtgtcggg cggggtgtcc
    22981 aacgtcagct tttccttccg cggcaacgac ccggtgcgcg aggccatcca caccgtgttc
    23041 ctctaccacg ccatccgcgc cggcatggac atgggcatcg tcaacgccgg catgatcggc
    23101 gtctacgacg agctggatcc cgagctgcgc cagcgcgtgg aagacgtggt gctcaaccgc
    23161 cgccccgacg ccgccgagcg cctgctggac atcgccgagg ccgccaaggg cgcggccaag
    23221 gacgattcga agaagctgga gtggcgccag gccccggtgc gcgagcgcct gacgcacgcg
    23281 ctggtgcacg gcatcaccga cttcatcacc gaagacaccg aacaagccta ccggcagatc
    23341 ctgcaggatg gcgggcgccc gctgcacgtc atcgagggcc cgctgatgga cggcatgaac
    23401 gtggtcggcg acctgttcgg cgccggcaag atgttcctgc cgcaggtggt caagagcgcg
    23461 cgcgtgatga agcaggccgt ggcccacctg ctgccctaca tcgaggccga gaagcaggcg
    23521 ctggcggccg ccggcggcga cgtgcgcagc aagggcaaga tcgtggtcgc caccgtcaag
    23581 ggcgacgtgc acgacatcgg caagaacatc gtcaccgtgg tgctgcagtg caacaactac
    23641 gaggtcgtga acatgggcgt gatggtgccc gcgcacgaaa tcctggccaa ggcgcgcgag
    23701 gaaggcgccg acatcgtcgg tctgtcgggc ctgatcacgc ccagcctgga ggaaatgcag
    23761 catgtggccg gcgaaatgca gaaggacgcc tacttccgcg aacgccagat tccgctgctg
    23821 atcggcggcg ccaccaccag ccgcgtgcac accgcggtca agatcgcgcc gcactacgac
    23881 ggcccggtga tctacgtgcc cgacgcctcg cgcagcgtcg gcgtggcgca gggcctgctg
    23941 ggcgcagacg tggcgcgcta ctgggccgac gtgcgtgccg actacgagcg cgtgcgccag
    24001 ctgcacgccg gcaagaagca ggcgccgctg tggccgatcg agcgggcgcg cgccaacaag
    24061 gcgcgcatcg actggggcaa ctacaccccg ccggtgccca agttcatcgg ccggcgcgtg
    24121 ttccagcaca tcgagctggc cgagctgctg cccttcatcg actggacacc gttcttccag
    24181 acctgggatc tggccggcaa atacccggcc atcctgaacg acgaggtggt gggccaggag
    24241 gccaggcgcg tgttccagga cgcgcagacg atgctgaaga agatcgtcga cggccgctgg
    24301 ctcaccgcca acgcggtggt cggcttctgg ccggccaaca gcgtgcacga cgacgacatc
    24361 gagctgtacg ccgacgaggc gcggcagcac gtggtgctga cctggcacgg cctgcgccag
    24421 cagagcgaga agcaggaaga cgccgccggc cagctgcgcc ccagccgctg tctggcggat
    24481 ttcgtagccc cccgaaatgc tattgattca gtagctgatg gcgcttgtca gtcgggctct
    24541 ggaggccaaa atgccttcaa ggactacctg ggggcctttg ccgtcaccgc cggcatcggg
    24601 gtggaagaca aggtggccga attcatggcc gcgcacgacg actacagcgc catcacgctc
    24661 aaggccctgg ccgaccgcct ggccgaggcc tgcgccgaat ggctgcacca ccgcgtgcgc
    24721 accgacctct ggggttacgc cgccgacgaa ggcctgagca acgaggagct gatcgccgag
    24781 cggtaccgcg gcatccgccc ggccccgggc tacccggcct gccccgacca cagcgtcaag
    24841 cgcccgctgt tcgagctgct gcgcacgcag gagatcggca tggggctgac cgagtcctac
    24901 gccatgacac cgccggccag cgtgagcggc ttctatttca gccacccgca gagcagctat
    24961 ttcaacgtcg gacgcatcga catgacgcag gtggccgaca tggcggcacg ccgcggcatg
    25021 gcgccggcgg cgctggagcg cctgctggcg cctaacctgg gcctgtgagg gcgggggcgc
    25081 cggtcacaac tcgaacacgt ggccggcgct gagccagcgg atgtcgtgcg ccacctcgcg
    25141 gcgcggcgga cgcacctggt cgaactgggc gatctcgcgc atgatggttt cggtgtcggc
    25201 cggcttggtg tgggtgatgt agatgggaaa gcggtgccgg cggtcgatcc ggtccagctc
    25261 ctcggccagc gtctcgggcg acaggtgctg ggccatctgc gccagctcgc gttcgcggtt
    25321 ggcgaaggcg gtttcgatca ccaggctgtg caccctgagc tggttgaggc gctgccagaa
    25381 gtccgggtgc ccgctggtgt cgccgctgaa cacccaccag ggcgcgtcgg tcgcctgacg
    25441 gcgcagcgca aagcccagcg ccggcaccgc gtggcgcgcc ggcagcgcct cgatgacgcg
    25501 cccgcccagc tcacgcgtgt gccccacctg cagcggatga aactggatca gcggcagctc
    25561 gggcgtgggc aggcgcgaga aatccggcca gatgacgtcg ttgagcacat gcgcgcgcag
    25621 cgcggcaatg gtctgcggca gcgcatgcac atgaatcggc cggccgatgc gatcgcaggc
    25681 catggtgtcg accatcagcg gcagacaggc cacatggtcg aggtgcgaat gcgtcagaaa
    25741 cacatgctcg atgcgcagca tctcgtcgcg cgtgagttcg ccgaccccgg tgccggcgtc
    25801 gatcaggatg tcctgatcga ccaggaacga cgtggtgcgg cagtcgcgcg caatggcgcc
    25861 ggagcaaccc agcacgcgca ccttgatggc agattgggtg gttgtcattt cacgtcgaac
    25921 accgtggtcc catcccaatc cggaccgggt ggaaaacgaa gcaaaaagac taacctttcg
    25981 gcatagagcc ggtaaaggta atagttggca tttcgcgcct gcagcgcctc gactgccgat
    26041 ccgaactcgg caaagtgtcc ggcgcgccac aggctcaggg cctgccgcca ggcggccagc
    26101 tcctcctgca acgccacgtc gtcctggccc gccgccgccc gcacggtgtg gatgctgacc
    26161 gcctggtggc gacccttgac gcgcacccga tcgagttcct gccagacatg gccagccgct
    26221 gcggcggcgt cccgggtggc ctggctggcc accaggtcga cctcgtacac gcgcgacagc
    26281 gcctccagcc gggccgccag attcaccgcg tcgccgatca ccgtgtaggc gcgccgcagg
    26341 tcggagccca tgttgcccac cgacatcacg ccggtgttca ggccgatgcc caccgacacc
    26401 gccggcagac ctcgggcggc gtggtcggcg ttgaggtcgc gcaggctggc gacaatggcc
    26461 acggcggcat ccaccgcgcg tcgcgcgtgg tccggcatgg cgaccggcgc gccccaaaag
    26521 gccatgacgc agtcgccgat gtatttgtcg atcgtgccct gatgcgtctg gatcacctcg
    26581 gtcaggtgac tgagcacttc gcccagcaag gtctgcaagg tctgcggctc catggtttcc
    26641 gccagactgg tgaaaccatg caggtcgcag aacattaccg tcagttcctg cgagcgcgcc
    26701 gccatgccgt agcgttcggg ctgctgcagc atctgctgca ccagttcggg cggcacgtag
    26761 gtggcgaact ggcgcgccag ggcgcgcttg gcccggccct cgacgaagta gcccagtgcc
    26821 atatcgagcg tcagcgcaat taggatcagc gccagcggtg tggccagcgg cagggccagt
    26881 ccggcgccga cgtacaggct gaggtccagg gccagcaggg ccgccagtag cgccgcgccc
    26941 aggccgagcg ccccacccac cgacagcagc ggcaagccca gcgcgagcac cccgcccagc
    27001 aacaccagca ggccgacctc gtacccggcc gcgtagtccg gccgcaccgc cacgcgcccg
    27061 tccagcatgc cggagatcag gttggcgtga atctcgaccc cagggtacac ctcggccacg
    27121 ggggtggcgc gcagatccat caagccggga gcggtaaagc ccagcagcgc gtagcgcccc
    27181 ttgagttcgg ccggcgcaag ccgcccctgc agcacatcgc tggccgagat gtatcgaaac
    27241 gacccgccct gaggcccgcc cggcccgcgg tagggcacca gcaccgttcc gcgatcatcc
    27301 accggaaggc gccaacggtg cacatcgtca cccagccaca gagcctggag cggcgcgtcc
    27361 gcctctgggc cgaggcgctc ctcacgcagt tgccgtgcgc ccaggccaag gcgcagcgtg
    27421 gccaacgaga gtgagggata caacgcccct tcgaaccccg ccaccagggg taccgcacgc
    27481 acgctgccat ctgggtcggc gacagcgttg aagaaacctg cagtccgggc cgcgccgttc
    27541 agcacggcga tattgccgcc gtaaccatcc cagtgcagca gccccctcgg gaggcgatcc
    27601 ggccgaatcc agggttcggg cagcgcgccg gtgcggctgc cagcgcggtc gctgctgaag
    27661 tagtacccca gcgccaccgg agcccgtgcc aggcgggccg ccagcaggcg gtcgccatcc
    27721 aatgtcggcg cggtgcgcgc cagccagtcg agaaacgccg ggttgtcgcg cagttccccg
    27781 cgcgccaacc gctccaaggc acccagtcca ccacccgcat cgagctcggc aaacaccacg
    27841 tccaacccca gcgcagccac ctgctgacgc tcgatcagtt cctcgatcaa ccgagcgacc
    27901 tcggcccgtc gccagggcca ctgaccatgg cgcgccagac tgggctcgtc gacgtcgatg
    27961 atgaccacgc gctcgtcgag cgtgcccggc atggtcagcc gcagacgcag gtcgtacaac
    28021 acctcgtcga gccgatccag caggggcgcg cgccacaccc ccactgcgtg caacgaggcc
    28081 agcagcacca gcagcgcgct ggccagcatg cgccggcgat gtcgctgaaa cccttgccaa
    28141 gccatgattc aggggcatgc gccgtcagga aggcgcgcgg catggccgtt tggcctccgc
    28201 ggtggatgac cactgcgatc cggcatgcgc ctgttcagcg ctcgaggaat tccagccgaa
    28261 ctccgcccaa ctccatctgg tctctgtcca ccagtgtgat agggcccaca tcgacggtga
    28321 cgccattgac cctggccatc tgttgccctt ccacctgagc caggtcgaac ccatgtgact
    28381 tgcgagtgat ggacgccacg cagatacccg gcttgccgat ggtagtgaca accttggtga
    28441 gcggcagttc acgtccggcc gccaggccac tgagcacacg cacgcgcggc ccgaccagtt
    28501 gctcggcgct gggcgcagga ggcgcatcga tgcccatggg cagcgacaga ccgatggcgg
    28561 tagcgctgtc gctgtggatg ccgactggct ccggagacgg cgcgctcgct tcaccatcca
    28621 ggaaccggat tttgtacttg ccgatctcga tgctgtcctc gctctccagc acctcactct
    28681 tgaccgatct gccgttgaca taggtaccat tggtgctgcc cagatcctcg atggtgacct
    28741 ggccagcgcc catctggagg acggcgtgtt ccccgctgac ggcaaggttg tcaatgacca
    28801 cgtcattgta aggtcgacga ccaatggtgg tgcgatcctt ggtgagttgg acctccttga
    28861 ccaccacccc gccgatcgaa acgatcaact tcggcatgaa gactccctgt tttttggccc
    28921 gaaccgatgg ccgacgagca attggacctc agggatcgac ccgcggcaag ccaactgttt
    28981 cctatttcct cagcaatttc gccatcattc cgggctttct gggcggttcc tgcgccttga
    29041 ccacgatcac cgagacattg tcacgaccgc cacgcccgtt ggccagttcg atcaggtgct
    29101 ggctcatctc gtgcggcgac tctgcgctac cgagcaccgc agcgatgtcg gcgtcgtgca
    29161 acatgtcgtt caagccgtcc gagcaaagca gcagcacgtc cccggaagca atcccaacct
    29221 cctgcacgtc cagcagcacc gtgtcctcga ccccgagggc gcgcgtcacc aggttgcggt
    29281 aacccgaagc tgcggcatct gccggcgtga tcaggccgat gtcgatctgc tcctgcaaca
    29341 gggaatgatc gcgcgtgagc agcgtcagca caccggcacg ccagcgatag gcccgcgaat
    29401 ctcccacatg gccgaccaac agggtactgc ccagcacagc ccccatcacc agggtggtac
    29461 ccatgccagc gcagtcgtcg ttggtgttgg cggcctcgaa gatcgcccgg ttggcgttgt
    29521 cgacgcagat ttccatggct cggcgcacgt cgcgcaaggt ggccgcggcc gccgcctcgt
    29581 gcaaccagcg acccagttcc tcgacgatga ggtcgaccgc catggcgctg gccacctcgc
    29641 cggcgttgta gccccccatg ccatcggcca gcacgacgat gccatgctcg gcgtcgacgc
    29701 gcaccgcgtc ttcgttgttg gcgcgcacgc ggccaacgtc ggtcagggcg ctgtagaaaa
    29761 actccatctc cgtgaccggt ggcctggcgg atcggggacg aagcatgcgc gttgcagcgt
    29821 gtgaggccgc ccgctcacgt cacggccgaa gccgaagcgc gccgacgctc gaggaacttg
    29881 cccagcgcca gcaccagcag cgcgcccacc acgcccatgc cgtagtgcca cgcccggtcc
    29941 tgcgggatga acgttgccag ggccgggtcg ctctgcgcca tggtgccggc gatccagccc
    30001 agcagcatgc cgcccagcag gatgatgacg gggaagcggt ccatcagttt gagcaccagc
    30061 tggctgcccc agacgatgat gggaatgctc accagcaggc cgaagatgac cagcgccagc
    30121 tggtgctgtt cgcccgcacc ctgcgcggca ccggcgatgg cgatcacgtt gtcgacgctc
    30181 atcaccaggt cggccacgat gaccgtcttg acggcgcccc acagcttgtc gctgctctga
    30241 atgccgtcgt gaccgtcctc ctcctcggga gcgatcaact tgaccccgat ccagatcagc
    30301 agcagcgcgc ccaccagctt gaggaacggg atctgcagca gcgccagcgc gaacgcgatc
    30361 aggatgacgc gcagcacgat ggcgccgacc gtcccccaga tgatgccctt ggtgcgctgg
    30421 tgcggtggca ggccacggca ggccagcgcg atgacgaccg cgttgtcgcc ccccagcagg
    30481 atgtcgatca tgatgatctg gccgacagcc agccagaaat gggcggtcag gaagtcttcc
    30541 acggggcgtc tttcttgtcg gggctgcgag cggcttccga aatgatacag ccggaccgcc
    30601 cgtatggcgt ccggctggcg ggcggttgcg cgtcagcgca acgtggtcac agcagcgcct
    30661 tgagcagccg ccccatttcc gaagggttgc gcgtgatctt gaaaccgcac tcttccatga
    30721 tcgccagctt ggcgtcggcc gtgtcggcgc cgccggagat cagcgcgccc gcgtggccca
    30781 tgcgcttgcc cggaggggcc gtgacgccgg caatgaagcc gacgatcggt tttttcatgt
    30841 ggtctttgca ccagcgcgcg gcttcggcct cgtcggggcc gccgatctcg ccgatcatga
    30901 tgacggcgtc ggtgtcgggg tcgtcgttga aggccttcat gacgtcgatg tgcttgaggc
    30961 cgttgatcgg gtcaccgcca atgcccacag cgctcgactg gccgatgccc agctcggtca
    31021 gctgcgccac ggcttcgtag gtcagggtgc cggagcgcga caccacgccg acgcggccct
    31081 tcttgtggat gtggcccggc atgatgccga tcttgatctc gtcgggcgtg atgacgccgg
    31141 ggcagttggg gcccagcagc agggtcttct tgccgcccct ggcctctttt tccttcatgc
    31201 ggttgcgcac ttcgagcatg tcccggatcg gaatgccctc ggtgatgcag accacgaagt
    31261 cgaggtcggc ttccaccgct tcccagatgg ccgcggcggc accggctggc ggcacgtaga
    31321 tcacgctgca ggtggcgccg gtttcacggg cggcgtcctt gacggtggcg aagataggga
    31381 tgttgaagat gctctcgccc gccttcttgg ggttgacgcc ggcgacgaag cagttcttgc
    31441 cgttggcgta ttcctggcac ttttcggtgt gaaactggcc ggtcttgccc gtgatgccct
    31501 gggtgatgac cttggtgtgc ttgttgatgt agatcgacat ggctggtggc tcctcaggct
    31561 tggacggcgg caacgacctt ctgggccgct tcggccatgg tgtcggcgct gatgatgggc
    31621 agaccggact cggccagcat cttcttgccc agctcttcgt tggtgccctt catgcgcacc
    31681 accagcggca ccttcaggtc gaccgcacgg ctggcggtga tgacgccggt ggcaatggtg
    31741 tcgcacttca tgatgccgcc gaagatgttg accaggatgg ccttgacctt ggggttgcgc
    31801 agcatgatct tgaaggcctc ggtgaccttc tcgggcgtgg cgccgccgcc cacgtccagg
    31861 aagttggccg gctcggcgcc gaacagtttg atggtgtcca tggtggccat ggccaagccg
    31921 gcaccgttga ccaggcagcc gatgttgccg tccaggctga tgtaggccag gtcgaacttc
    31981 gaggcttcga tctcggccgg gtcttcctca tcgaggtcgc gcagggcgac gatctcgggg
    32041 tggcggaaca gcgcgttgct gtcgaagttg aacttggcgt ccagggcgat gatgttgccc
    32101 ttgctgtcgc ggttcagcgg gttgatctcg accagcgagg cgtcggtgtc catgtagcac
    32161 tggtagagct tgcggcacac gtccacgaac tgcgccgccg agtcggccgg catgcccacg
    32221 ccgcgcgcca gttcctggcc ctgcgcgtcg gtgaagccca cgagcggatc gacaaacacc
    32281 ttgacgatct tctcgggcgt gctgtgcgcc acttcctcga tgtccatgcc gccctcgctg
    32341 gaggcgatga aggccacctt ctggctggcg cggtcggtca cgaccgacag gtagtattcc
    32401 ttctggatgt cggcaccgtc ctcgatgtac aggcggcgca ccttctggcc ctcggggccg
    32461 gtctggtggg tcttgagctg catgcccagg atctggccag ccagttcctt gacctgttcg
    32521 agcgacttgg ccaccttgac gccgccgccc ttgccgcgac cgccggcgtg gatctgggcc
    32581 ttgacgaccc acacggggcc gcccagcttc tgcgccgcct cgacggcttc ctgaacggtg
    32641 aacgcgggga tgccgcgcgg cacgggcacg ccgaactgac gcaagatctc cttgccctgg
    32701 tactcgtgga tcttcatgag gcccctttga ggatgggtga gtgagtggaa agcccgtccg
    32761 ccatgccgga cggtgcgcaa ataacacggc cgcacccacg ctacgactcg aagcaggcgt
    32821 cagtcgaggc ctgcgaatgt atcatgttgc ggcgcaccaa ttctcccact ggggcttacg
    32881 tcgccttgac gtcggccaca tgaaaggacc gcccatggcc aaggttttca ttgacggcga
    32941 agccggcacc accggtctgc agattcgcga gcgcctgcag ggcatggccg gcgtggaact
    33001 gctcagcatc gatccggcgc ggcgcaagga cccgcaggcc aagcgcgacc tcatggcggc
    33061 ggtcgatctg gtcgtcctgt gcctgcacga tgacgcggcg cgcgagtcgg tggcgatgat
    33121 cgacgcgctg ggcgagcgcc ggccgcgcat catcgacgcc agcacggcgc accgggtggc
    33181 gcccggctgg gtgtatgggt ttcccgagct gtcggcgcag caacgcgccg ccgtgcaggc
    33241 cgccgagcgg gtggccaacc cgggctgcta cgccaccggc gccatcgccc tgctgcgccc
    33301 gctggtggac gccggcctgc tgccgccgga gcatccggtg gcgctgccct ccgtgagcgg
    33361 ctacaccggc ggcgggcgcc agatgatcga agcctacgag gccggcaacg ccccgcccta
    33421 cgagctgtac gcgctcggcc tgaagcacaa gcacatcccg gagatcatgc gctacggccg
    33481 cgtcacgcgc cgcccgatct tcgtgccggc ggtgggcaat ttcgatcagg gcatgctggt
    33541 ggagttgccg ctgcatctgg acctgctgcc gggccagccc agcgccgagc agctgctgcg
    33601 cacctatcgc gcccactacg ccggcgccga ggcggtcagc gtggaggcgg cgcccgaatc
    33661 cggcaagctg gatgcggtgg ccctcaaggg caccaaccag ctggaaatcc gcgtgttcgc
    33721 caaccccgag gccgcgcagg cggtgtgcgt ggcgcgactg gacaacctgg gcaagggtgc
    33781 cagcggagcg gcggtgcaga acctgaagct gatgctcgga ttggactgag cctgaaaaca
    33841 gccaccgcac cgcccggcga tgccggcgag cgtgcgcgcc agcaaatgac cctaggaggc
    33901 tgtgggactt ggaaatcgcc ggctgcaaat cgaccgcagc ggcccatttt tggcttcgcc
    33961 gctcttcgag agcaccgtct ttttgaccaa tagctcgact cttgggctgc aaatccggca
    34021 aaaactgggc tcgctggggc cgattttggc tacgcagatc ttcgagaacg ctttcggcgc
    34081 tccaagcccg acagcctcct aggatgacag acggtcaacc gccgttgctg gggcctgtcg
    34141 cctgcgccgc cgcactcaac acgcgccagg cgacttctgc gctgaagccg cgccccgcca
    34201 ggaagcgcag ctggcgcgca cgctcgccgg catcgccggg tggtgcggcg aagcggcggc
    34261 gccagaccgc cagcgcacgg tcgaactcgc tgccctgcaa ctggcgcgcc gcgtcggcga
    34321 ccaccgcctc gggcaagccc ttggtgcgca actcgtgcag cacgcgcgcg ctgcccagac
    34381 ggggcgcacg acggtgcagc accgattcgg ccacccgcgc ctcgtcgatg aagcccttgg
    34441 ccgccagctc gtccagcaca cgctcgatcg ccgcgggcga ttcatgctcg gtcgcatggc
    34501 gcgccagctt ggtcgccagt tcggcgcgtg aatgttcgcg cgccgccagg tagcgcagcg
    34561 cgcgcgcctt cagcgaaagg ggcgccctgg ccatggcgtc agttggcgat cacccgcgcg
    34621 gccggccgtg ccgggcaacg ctgacgggac cggctcagga tggcctcaaa cctcatcggc
    34681 gtcggccagc gccgccggtc gctgggccac gcccagcgac tcgcgcacct tgttttcgat
    34741 ctcgcgcgcc agttcggcgt tttcgcgcag gaactcgcgc gcgttgtcgc ggccctggcc
    34801 gattttttcg ccgttgtagg cgtaccaggc gccgctcttg tcgagcacct tggcggtcac
    34861 gcccatgtcg atgatctcgc cctcgcgcga aatgccctcg ccgaacagga tgtcgaattc
    34921 ggcctgcttg aagggcgggc tgaccttgtt cttgaccacc ttgacgcggg tttcgttgcc
    34981 gatcgcttca tcgcccttct tgatggtgcc gatgcggcgg atgtccaggc gcaccgaggc
    35041 gtagaacttg agcgcgttgc cgccggtggt ggtttcgggg ctgccgaaca taacgccgat
    35101 cttcatgcgg atctggttga tgaagatcac cgtgcagttg gttttcttga tggtggccgt
    35161 cagcttgcgc agcgcctggc tcatcaggcg cgcctgcagg ccgggcaggc tgtcgcccat
    35221 ctcgccctcg atttcggcct tgggcaccag ggccgccacc gagtcgacca ccaccagatc
    35281 gatggcgccc gagcgcacca gcgaatccac gatttccagc gcctgctcgc cggtgtcggg
    35341 ctggctgatc agcatgtccg acaggttgac gccgagtttt tgcgcatact gcgtatccag
    35401 cgcgtgttca gcatcgatga aggcgcaggt gccgcccagt ttctgcattt cggcgatgac
    35461 ctgcagcgtc agcgtggtct tgcccgagga ttccggccca tagatctcga tcacccgtcc
    35521 gcgcggcagg ccgcccacgc ccagtgcgac gtccaggccc agcgaaccgg tggagaccac
    35581 ctggatgtcc tcgatcgcct cgccctcgcc caggcgcatg atggtgccct tgccgaattg
    35641 cttttcgatc tgcgccagcg cggcctgcag ggccttggct ttctcgttct tgtcggcgac
    35701 ggccatgtcc atgattttct ccttgtgtat caagtgctta acgaatcccg gatgcccgcc
    35761 gtggcggctg gctggatgtt tgaacaggac tgtacatcaa tccattcaaa gacacaaagc
    35821 tgatttttgg tcagtttgcc ttatcatttg cgcatgtcgc ccgatgcccc tgaagaccgc
    35881 tggcgccaga cccacctggg ccgcctgctg ggccacgcca tgcggcgttt cgacgcccgc
    35941 gtgctgcagc tgatggccgc cagcgagcgc gcaccgctgg cgctgtccaa cctggccgcg
    36001 cgcgacaagg tgggcgcggc catcgtgcac gtcacgcgcc atctgccgct gaccggcgcg
    36061 cgcctgaccg agctggcggc ctgggccggc atgagcaagc aggccatggg tgacctggtg
    36121 gaccagtgcg tggcctgggg cctggtcacg cgcgaaggcg acccgctgga cgcccgggcg
    36181 cgccgcatcg ccttcacgcc tgccgggctg gactggctgg cagcgttccg tgacgccgtg
    36241 gcgcaggccg aggccgagtt ccgcgccgcc gtgggcaccg aggtggccac cgtggtggcg
    36301 ctggggctgg aggcctacgc cgcctgaagc gtgccgcgcg cccggccctg ccggcgcagg
    36361 gtcaacccca gcgccgcagg cgtcggtctc ggccctagaa tttccctcgg cacaaccgag
    36421 aatcatgacg gccgcctccg cagccgcccc aaaggagaca agcatgcgca tcctcatcgc
    36481 cgaagacgac caggtgctgg ccgatggcct gatgcgcggg ttgcgcaatt ccggcgccgc
    36541 cgtcgaccac gtggccagcg gcagcgaagc cgacgccgcg ctgatgacca acgccgagtt
    36601 cgacctgctg atcctcgatc tgggcctgcc caagatgaac ggcctggaag tgctgcgccg
    36661 cctgcgcgcg cgcgggcaga cgctgccggt gctgatcctg accgccgccg acggcgtgga
    36721 agagcgcgtc aagggcctgg actacggcgc cgacgactac atggccaaac cgttcgcgct
    36781 gtccgagctg gaggcgcgcg tgcgcgcgct cacgcgccgc ggcatggggg cggccagcgc
    36841 catcatccgc cacggcccgc tggaatacga ccagaccggc cgcgtagcca gcatcgacgg
    36901 caagatgatc gagctgtcgg cgcgcgagct gggcctgctg gaggtgctgc tgcagcgcag
    36961 cggccgtctg gtgagcaagg agcagctggt cgagctgctg tgcgaatggg gcgaggaagt
    37021 cagcaacaac gcgatcgagg tctacatcca ccgcctgcgc aagaagatcg agaaggggcc
    37081 gatccgcatc gccaccgtgc gcggactcgg ctactgtctt gaaaaggtat cagcctgaaa
    37141 gcgccccagc gcccgcccag acagcgcaag cagctataga aactgaagtg tcccggtgaa
    37201 actgttccag cgcaaccagc gctcgctgtt cggcgagatt ctggactgga tgctcacgcc
    37261 cctgctgctg ctgtggccca tcagcctggc gctgacctgg ctggtggcgc agaacctggc
    37321 caacaagccg ttcgaccgtg cgctggtcta caacgtgcag gcgctggcgc agttcatcca
    37381 ggtcgggccg cgccagcgcg cgcgcttctc gctgccgcag ccggccagcg agctgctgcg
    37441 cgccgacgac gccgacaacg tctacttcca ggtgatgggg gcgcacggcg agttcctggc
    37501 cggcgagcgc gagctgcccg caccgcccgc acccggcctg agcgaagacg acgccgcgct
    37561 cagcgacatg ccgcagctgc gcgacgccga gttccgcggc ctgccggtgc gcgtggccta
    37621 cctgtgggtg ccgattgccg gcgccccgca gccggcgctg gtgcaggtgg ccgaaacgcg
    37681 cgaaaagcgc agcgtgctgg cgaccgaaat catcaagggc gtgatgctgc cgcagttcgc
    37741 catcctgccg ctggcggtgc tgctggtgtg gctggcgctg gtgcgcggca tccgcccgct
    37801 gtcggagctg gagtcgcgca tccgcgcgcg ccggcccgac gacctgtcgc cgctggacga
    37861 ccacgccgtg ccgcaggagg tggcgccgct ggtggcctcg gtcaacgacc tgctgacgcg
    37921 gctgaaggac tccatcgcca cccagaagcg ctttctggcc gacgccgcgc accagctcaa
    37981 gaccccgctg gccggcctgc gcatgcaggc cgagctggcg cagcgcgagg gcgccagcga
    38041 ggaggagctc aagcaatcgc tgcagcagat cggcctcgcc agcgtgcgcg ccacccacac
    38101 cgtcaaccag ctgctggcgc tggcgcgggc cgaaggcggc agcgcggcgc tggcgcacga
    38161 gccctgcgac ctggccgcgc tgaccatcga agtggtgcgc caggcggtgc cgacggcgct
    38221 cgaacacggc accgacctgg gctacgaagg ccccgagccg ggcgcgccgc acctggtgcg
    38281 catgggcaac ccggtgctgc tcaaggaaat gctgcgcaac ctggtggaaa acgcactgca
    38341 ctacgcgcgc acccccggca ccggcgccgc ggtggtgacg gtgcggctgc gcgccaaccc
    38401 gccgggcggc ggcctggcgc tggaggtcga agacaacggc cccggcgtgc cggcggccga
    38461 gcgcgagctg gtgtttcagc ccttctaccg cgtgctgggc accaacgtcg acggcaccgg
    38521 cctgggcctg ccgatcgtgc gcgagatcgc cgagcagcat ggcgccagcg tcagcctgag
    38581 cgaggcccgc ccgggccagc agccacccgg cgcgcgcttc gtggtgcgct tcatggcgcc
    38641 ggcgtgacgc ggcgctgccg gtcacgccgt cgtcacttga ttattgatag ctgctggtgc
    38701 ttgctggacg ggcggcgagg tcgtttctgg cattgattca caacgcggca tcacttaccg
    38761 atgcaaaagc gcgcaaagat ggcgcccagc aggtcgtcgg cgctgaaggc gccggtgatc
    38821 tcgcccagcg cctgctgcgc caggcgcagc tcctcggcca gcaggtccag cgcggcgctg
    38881 ccgtctagct gcgcctgcgc ggtctgcagg tgctcgtggg cgcgcgccag cgcctgcaga
    38941 tggcgctggc gcgcgatgaa caggccctcg ccggcgccgc cctgccagcc gaccagctcc
    39001 agcaggcggc ggcgcagcgc gtccagcccc tcgccggtgc gcgccgacag ggcgatgtca
    39061 cccggcgcga tggcggcgtg cggcgccgcg tcgtgcttgt tccacaggtg cagcaccggc
    39121 accccggcgg gcaggcggtc ggccaggccg cgcgcgatct gggcttcggc ggcggcgtag
    39181 tcgggcgcgc cctggcgcgt caggtcgtgc agccacagca cggcgtcggc ctgttcgatg
    39241 tggccccagg cgcgctcgat gccgatgcgt tcgacctcgt cgccggcttc gcgcaggccg
    39301 gcggtgtcga gcacgtgcag cggcacgccg tcgatctgga tggtctggcg caccgcgtcg
    39361 cgcgtggtgc cggcgatcgg cgtgacgatg gccagctcgg cgccggccag cgcgttcagc
    39421 aggctgctct tgccggcgtt gggctggccg gcgatcacca cctgaatgcc ttcgcgcagc
    39481 aacgcgccct ggcgcgcgcg ggcccgcacc tgggtcagcg tttgctgcaa ttttgatagc
    39541 tgcccgggca catcgtgggc ggccaggacg tcgatttctt cttccggaaa atcgagcgtg
    39601 gcctcgatca gcaggcgcag ctggatcagc ccgtccacca gctggcggat ctggcgcgag
    39661 aactcgcccg ccagcgaacg gctggccgag cgcgcggcgg cggcggtgct ggcgtcgatc
    39721 aggtccatca ccgcctcggc ctgggccaga tcgagcttgt cgttgaggaa ggcgcgctcg
    39781 gtgaattcgc ccggccgcgc cagccgcagg tgcggcagcc gtggtgcgcc gcgcaccgga
    39841 tcgggctcgg ccgccgcctg caggcagcgc gccagcaaca gctgcagcac caccgggccg
    39901 ccgtgcgcct gcagctccag cacgtcttcg ccggtgtagg aatgcggggc cggaaagaac
    39961 agcgccagcc cgtggtcgat cgggctgccg tcggcctcgg tgaacggccc gtagctggcg
    40021 tggcgcggcg tcagcacgcg cccggtgagc gcctgcgcca gcggccccag gccggccccg
    40081 gacacgcgca cgatgcccac cgcgccgcgc ccgggggcgg tggcgatggc gacgatgggg
    40141 tccggcgcag cggctggcat ggcatgattg tccgccacct gcctgccctt tcacccgccg
    40201 ccgccatgga ccgcctcctg attcagaacg tgcatgtgcc ggacctgcgc cgcggcaccc
    40261 ggcgggcgca ccaccaggtg ctggtggagg gcggcgtgat ccgcgccgtc accgacgagg
    40321 cgctgccggc cgccggcgcg cgcgtgctgg acggcggcgg gcgcaccctg ctgcccgggc
    40381 tgatcgactg ccacgcccac gtcaccttct cacagaccaa cctggccctg ctggccgaca
    40441 tgccgcagac caccctggtg ctgcgcgcgc tgccgatcct gcgcggcatg ctggggcgcg
    40501 gcttcaccac cgtgcgcgac gccggcggcg ccgactgggc gctcaagcag gccatcgacg
    40561 acggcctgga tggcagcgtg ctcgggccac gcctgttcgt cagcggcccg gcgctcagcc
    40621 cgaccggcgg gcacggcgac ttccgcccac gctcggaccg gctgcggccg ttgggctgcg
    40681 gctgccagcg cctggccagc acccgcgtgg tggacggcgt ggacggcgtg cggcgggcgg
    40741 tgcgcgagct gctgcagatg ggggccgacc agatcaagat catgtcgtcg ggcggcgtgg
    40801 cctcgcccac cgacccgatc gacgcgctgg gttattcggt cgaggaaacc cgcgccatcg
    40861 tcgacgaagc ggcggcacgc cacacctacg tgctggcgca cgcctacacg ccggcggcga
    40921 tccggcgcgc ggtggactgc ggggtgcgca gcatcgagca cggcaacctg atcgacgcgc
    40981 ccaccgccga ccgcatggcc gagcgcggcg cctgggccgt gccgaccctg atcacctacg
    41041 aggcgctggc gcagggcggc gtgcggctgg ggctgggggc cgacagcgcg gccaagatcg
    41101 agcgcgtgcg cgcggccggg ctgcgctcgc tggccatctt caaggccgcg ggcgtgaaga
    41161 tggccctggg caccgacctg ctgggcggct cccacgggct gcagagcgag gagctgctga
    41221 tccgcgccca ggtgcagtcg aacctggagg tgctgcaaag cgccaccctg atcggcgccg
    41281 aactgctggg ccaggccggc cagctgggcg aagtcagccc gggcgcgcgg gccgatctgc
    41341 tgctggtcga cggcgacccg ctgcaggatc tgcgctgcct gaccggccag ggcgaaggca
    41401 tcgcgctggt gatgcaggcc ggacgcatcg tgatcgaccg ccgcagcggc ccgacccgcc
    41461 cgccgaccga aggtcaatca ggcctctgac gccctgtcag caagcgcagg aagctattgt
    41521 tttgatagtc gcctgccgcc gcccacggcc accgccgcct tcccgcggcg gcgcgggagg
    41581 cgccaccgca gctcaggcgc cgatcacttg acggtggtcc agatcgccat cgggcggtcg
    41641 tcggcgcggt gcaccgtggt cacgcccttg cgcatggccc aggggcggat ctggtcgtgc
    41701 agcggcaggt agttgtagtt gtccttgacg atctgcagcg cctgatggat ctcttcggtg
    41761 cgcttcttgg cttccatctc gctcttgatc tgctggatca ggccgtcgac cttggggtcg
    41821 ctcatgcggc cgcagttgaa gttgccggcc gcgccgccct tggtgtcgac ggtgctgatg
    41881 agcgagtcga gcgagtacag ggcgtcgaag gtggccacgc cccagcccag caggtaggcg
    41941 ctgacctcga agcgctggat cttggccgag tggatggcca tcgggttgct ctgcagcttg
    42001 gtcttgacgc cgatcttggt ccacatcgag gtgatggcct ggcagatcgc ctcgtcgttc
    42061 aggtagcggt cgttggggca gtccagcgtc agctcgaagc cgtccggata gccggcgtcg
    42121 gccagcagct tcttggcggc ggcgacgtcg aacttggcgg cgcgcgtggc cagctccttg
    42181 gtccagccgt tgaccatggg cgcgatcatg gtgccggtgg gcttgcccag accgcgcatc
    42241 acgttgcgct cgatggcggc gatgtccacc gcctggtaga gcgcctggcg cacccgcttg
    42301 tccttgagcg ggttcttgcc cttgacgttg gagcccagca gctcgtcgcg gaactggtcg
    42361 aagccgatga agatggtgcg gttttcggcg ccctcgatga cgctgatgtt ggggtcgcgc
    42421 ttgatgcgct cgaggtcctg caccggcggg tcgaccacca tgtcgacctg gcccgaaatc
    42481 agcgccgccg agcgcgtggc cgccgccttg atgggggtga acacgatgct gtcgacgttg
    42541 cccttgggct tgtcccacca ggcgtcgttc ttcttcagcg tggtcttggt gtcgggctgc
    42601 caggcttcca agatgaaggg gccggtgccg ttggcgttgc gggcggcgaa gttttcttcc
    42661 ttggccttgt agtcctgcgc cttggtggcg ttgttcttct cggcccaggc cttgttcatg
    42721 atgcgcgcgt cggtcagctc gcgcagcacc accgggctgg gcgccttcag gatcacgtcc
    42781 accgtgtggt cgtcgacctt cttgacttcc ttgacgctct gcacgtaggc cgtcatgttg
    42841 gacggcggcg tcatggcgcg gttgagcgag aacaccacgt cgtcggcggt gaggggcgcg
    42901 ccgtcatgga acttgacgcc cttgcgcaat tcgaagcgca gctgggtcgg cgtgacgaag
    42961 gtccacttgg tggccagcga cggctcgacc tcgtattttt cggtgtagcg caccagcgac
    43021 tcatagacca tctgctggta ggccagggtg gtctggtggt tctgcgcgtg cggatcgaag
    43081 gtcaggatgt cgttggcgcc ggcgatgcgc agcgcggcgg cctgcgcggc ccccgccagg
    43141 gtcagggcag ccagcgccac ggcgccacga acgaacaggg gacgagccat gaaatcacct
    43201 tccagtgaag ttggaaagtg cccatgctac cgaagaattg gcatcgaatt cgggcttccc
    43261 ggggtttccc cgggggtcgg cattgtcgac gattactatg aattcaatag ctgcttgggc
    43321 acaacaggca agcgctggcg atcgaattct tcaacgttcc gatgccagtc gggtcaccga
    43381 cccaccgcgg ccggcgaggc gcgccggccg cggcggcaca gggctcagaa cttgggcagg
    43441 ttgaactgcg gcggcacgcc catgcgggtg ttgatgatcc actgctgggc gatggtcagg
    43501 atgttgttgg tcacccagta cagcaccaga ccggcgggga agaagaagaa catcacgctg
    43561 aacaccagcg gcatgatcca catcatcctg gcctgcatcg ggtcgggcgg cagggggttg
    43621 agcgcggtct gcagcagggt gctgagcgtc atcaccaccg gcaggatgaa gtacgggtcc
    43681 ttcaccgaca ggtccttgat ccacaggatc cagggcgcgc cgcgcatttc cacgctggac
    43741 agcagcaccc agtacagggc gatgaacacc gggatctgga tcatgatggg cagacagccg
    43801 cccatcgggt tgaccttttc ctcgcggtag atgcgcatca tctccatctg catctgctgc
    43861 gggttgtcct tcaggcgctc gcgcagctcc tggatgcgcg ggttgatggc cttcatcttg
    43921 gccatgctgg cgtaggcctt ggcgttgagc cagtagaagg cgatcttcag cagcaccacc
    43981 agggccacga tggcccagcc ccagttgccc agcacgccgt gcagcttgtc gagcagccag
    44041 tacagcggct tggccaggat ggtgaagtag ccgtagtcct tgaccagctc cagccccggg
    44101 gcgatgcctt ccagcacctt ctcgtcctgc gggccgatga acaggcgcgc ctgcaccgcc
    44161 tggctggcgc ccggggcgat ggcgcccagc gaagcgatca tgccggcggc gtacagacca
    44221 ccatcgagct tgcgcgcgaa gttgtcgtgc cccatgccct gcggcagcag ccaggcgctg
    44281 gcgaagtaat gctgcaccat cgccacccag ccgtcgggcg acggcgtggc gaactcggcc
    44341 ttgcccttgt cgatgtcggc aaactcgacc ttctggaact tcttggcgtc ggtgtagaag
    44401 gccggtccgg tgaaggtgga ataaaagctg gtgccgcccg gcgccgggtt gccgtcgcgc
    44461 accagctgca cgtacagctg agggctgacc ggctggccac cggtgttgac cacctcatgc
    44521 cgcacctggg ccacgtagtc gccacgccgc agcgtatagg tcttgaccag cttgaggccg
    44581 cccagctcgg gcgactcgaa gcgcagcacc aactcgctcg caccttcggc cagggtgcgc
    44641 tcgcccgtga agcgcatcgc cgtcttgtgg gtggggaacg gcccaccgat caggcccgac
    44701 tgcgcctggt aggtgcgctg ggggctgtcg tccagcagca cgaaggcctg gcccgggtgt
    44761 tcggtctggt cgaacttgag gaactcgctg cgcacgatag agccgccctc ggtgtcgagc
    44821 accagcttga gcacgtcggt cgtgacttcg atgcgctcgc gcggcgcagc ggcggcggcg
    44881 gccggtgcgg cgtcgccggg cggcacaccc gccggtgcca cccccggcac cgcggccggc
    44941 tgggcgctgg ccgccggcac cgccgacgcc ggcgcgctgg cctggctggc cggcgtgggc
    45001 gcgccgggga agaaggtcgc cttgcggccg ttgtgcacct gccactggtc ccacagcatg
    45061 accagcgaga aggcgaagat cacccagagg atggtgcggc gaatgtcttt catgacggct
    45121 gacgaggcgt tgacgggtcc gaccgctcgc ggtcgatcag acgggtgaaa aagcgcggcg
    45181 gctgcggcgg caccggatcg caaccgccgt cacagccggg gtggcagcgc agcagccggc
    45241 gcagcgtcag gtagctgccc gccagcgcgc catgccgttg cagcgcgccg atggcgtaga
    45301 gcgaacaggt cggttcgaaa cgacaggacg aacccagcca gggcgacagc agcaggcgat
    45361 aggccttgac cagcccgatc agcagcgccc gcatcaggag gcgctccggc cctgcgcgaa
    45421 cagctggcac agctcggcgc gcaccgcccg ctgcagcgcc accgacgcgg cgctggggta
    45481 ctgcgcgggg gcaaacgccg cccgcaggcg caccaggtgc gcccccgcgg ccagcggcgg
    45541 ctgcaggcgc tcgcccacgg cgtagatctg gcgacggatc aggttgcgcg tgacggcgcg
    45601 gcgcgcccag cgcttgggcg ccatggcgcc cagccaggcc acgccggcag gacagaacag
    45661 ccccggcgcg acggcgtcgg gcaggccctg cggccccgcc aggcgatgca gggcaaagtg
    45721 cgccgtgcgc gccaccggcc gctgcgccag cagggcctgg aactgctcac gcgtgcgcaa
    45781 ccggccaggc gtggccgacc gcagcgcagg cgcagcggtg gcgtcgacgc cgctcaccac
    45841 gagccgtccc aagccggcag gctcagaccg ccaggcgctt gcggcccttg gcgcggcgcg
    45901 cgttgatcac cgcgcgaccg ccgcgcgtct tcatgcggac cagaaagccg tgggtgcgcg
    45961 cgcggcgcgt cttggaaggt tggtacgtgc gtttcatgac gaatcgtcct tggagtaaat
    46021 gggcttgggg tcgtcccggc ccgtcgcggc atcttgggcg ccctgcgcgc ccgcccggcg
    46081 cccggccgca ccactggtgc ggccagcccc atggcatgcc ctgccactgg cgccacgcgg
    46141 ggaaggcggg ctcgcctgcc gcccggctgg cgcggacccc gtggccccag cgctcgcccg
    46201 gcccgaccca ggaaaacccg tcattatcgc aaattccgcc gtgccggcca aggggaaggt
    46261 catggcatgc gcgccggccc gcgacccgcg ggctttggca gacgctcttg tggccgcccc
    46321 cgacatgtgg ataattggcg tcgaccgacc cttttcggcg ctcctgcggc gccacttatc
    46381 cacaccacca gcacccccat gcacatcgat caaccccagc gcgccagcgc cagccacggc
    46441 gcggccttgt gggacacctg cctggaaacg ctggcgcgcg agctgcccga gcagcagttc
    46501 aacacctgga tcaaaccgct gtcggcccag ctggcggccg accacagccg cctgaccctc
    46561 tacgtgggca accgcttcaa gctggactgg gtgcgcgccc agtacgccac ccgcatcgcc
    46621 gccgtgctgg aggcgctgca gggccacgcc gtgcaggttg agttagcgct tgcacccagg
    46681 gcagcgccca tcaaaccgtc attctccatc ccaacatcgg aaaccaccca gggcgacccg
    46741 gagcccgcgc gggcgcccga gcccttgcct gcgccgaccg gcgaccgcga cagtgccggc
    46801 ccggtgcgca cccggctcaa tggcgcgctg accttcgaca ccctggtgga aggcacggcc
    46861 aaccgcatgg cgcgggccgc cgcgctgcac gtggccggcg cgccgggcca gctctacaac
    46921 ccgctgttca tctacggcgg cgtcggcctg ggcaagacgc acctgatgca cgcggtcggc
    46981 aaccgactgt tgcaaacgcg cccggacgcc aaggttctct acatccatgc ggagcagttc
    47041 gtgtcggatg tggtcaaggc ctatcagcgc aagaccttcg atcagttcaa ggaacgctac
    47101 cactcgctcg atctgctgct gatcgacgac gtgcagttct tcgccaacaa ggaccgcacg
    47161 caggaggagt tcttcaacgc cttcgaggcg ctgctggcca agaagagcca catcgtgatg
    47221 accagcgaca cctaccccaa gggtctgacc gacatccacg agcgcctggt gtcgcgcttc
    47281 gactccggcc tgacggtggc gctggagccg cccgagctgg aaatgcgcgt ggccatcctg
    47341 atcagcaagg ccgccgccga aggcgccgag atgccggaag aggtggcctt cttcgtcgcc
    47401 aagaacgtgc gctccaacgt gcgcgagctc gaaggcgcgc tgcgcaagat cctggcctat
    47461 tcgcgcttca accagaagga catctcgatc gccctggcca aggaggcgct gcgcgacctg
    47521 ctgtcgatcc agaaccgcca gatcagcgtc gagaacatcc agaagacggt ggccgactac
    47581 tacaagatca aggtggccga catgtacagc aagaagcgcc cggccagcat tgcccgcccg
    47641 cgccagatcg ccatgtacct ggccaaggaa ctgacgcaaa agagcctgcc cgaaatcggc
    47701 gagctgttcg gcgggcgcga ccacaccacc gtgctgcacg cggtgcgcaa gatcgccggc
    47761 gaacgccagc agctgaccga gctgaaccag cagctccacg tgctggagca gacgctcaaa
    47821 ggttgatggt tccgaagtcg aaaggccacg aaaaccggcg acaatgcggg gttggctcgc
    47881 gccggcccca gggagcggcg atgcgcggcg cgccgacggt catgacaagg atcaagagga
    47941 agacatgatt gttctgaaga ccacccaaga caggctgctg gccgcgctgc aatcggtcgc
    48001 cggcatcgtc gagcggcgtc acaccctgcc ggtgctggcc aacgtgctgc tgcgcaagag
    48061 cggcgacacc ctgcagctga ccaccagcga cctcgagatc cagatccgca ccgccgccga
    48121 actgggtggc gacagcggcc acttcgccac cacggtgggc gcgcgcaagc tgatcgacat
    48181 cctgcgcacc atgccagccg accagacggt gagcctggag agcagccagt ccaagctgat
    48241 cctcaagggc ggcaagagca agttcaccct gcagacgctg ccggccgagg acttcccgct
    48301 ggtgcaggaa gcgcccagct tcggcgcgtc gtttcaggtg ccgcagaaga ccctcaagac
    48361 cctgctcgac caggtggcct tcgccatggc ggtgcacgac atccgctatt acctcaacgg
    48421 catcctgttc gtggccgaag gcaagcagct gtcgctggtg gccaccgacg gccaccgtct
    48481 ggcgtacgcc agcgccaccc tggaggcgga cgtgcccaag caggaggtga tcctgccgcg
    48541 caagacggtg atcgagctgc agcgcctgct gtcggacgcc agcgtgcccg aggggcagca
    48601 ggcgcccatg atcgaaatgc agtttgccgc caaccaggcc aagttcagct tcggcgccat
    48661 ggaattcgtc accaagctgg tcgagggcaa gttccccgac tacaaccgcg tgatccccag
    48721 gaaccaccgc aacagcgtca ccctgggccg cgcgccgctg ctggccagcc tgcagcgcac
    48781 cgccatcctg accagcgaga aattcaaggg cgtgcgcctg aacttcgagc ccggcagtct
    48841 gcagatcgcc tccaacaaca gcgagcagga agaggcggtc gacgaaatcg atatcgacta
    48901 cgacggcgaa ccgatcgaga tcggcttcaa cgtcacctac ctgatcgacg ccctggccaa
    48961 catgaaccag caggagatgg tcagggtcga gctgcaggat ggcaacagct cggccctcat
    49021 caccctgcct gacaacgccc acttcaagta cgtggtgatg ccgatgcgga tctgagcgcg
    49081 ctcctgattc aggagctgcc cgcgcccgca acccgccgcc cgaggcgggt tttggctttc
    49141 aaatgagcga gaaacgacga tgaccgaccc ccgccccgac cccgaccagc ccgccgccgc
    49201 caccccatcc gccggggccg aaggctacgg cgaaggcgcg atccagatcc tggaggggct
    49261 ggaggcggtg cgcaagcgcc ccggcatgta catcggcgac acctccgacg gcaccggatt
    49321 gcaccacctg gtgttcgagg tggtcgacaa ctcgatcgac gaggcgctgg ccggccactg
    49381 cgacgacatc gtcgtcacca tccacagcga cggctcgctg agcgtggtcg acaacggccg
    49441 cggcatcccc accggcgtga agatggacga caagcacgag cccaagcgct cggcggccga
    49501 aatcgccctc accgagctgc acgccggcgg caaattcaac cagaacagct acaaggtctc
    49561 gggcggcctg cacggcgtgg gcgtgtcct
//
LOCUS       DAOQTZ010000008        49493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_791_length_49493_cov_14.050932, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000008 DAOQTZ010000000
VERSION     DAOQTZ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 49493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49493
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_49493_cov_14.050932"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1304)
                     /locus_tag="PKN51_01970"
     CDS             complement(30..1304)
                     /locus_tag="PKN51_01970"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT83896.1"
                     /translation="MAAAGAFDAVILGAGAAGLFCAARAGQRGLRVLLIDHAARLAEK
                     VRISGGGRANFTNRDLDPKAPQRHYLSANPRFCRSALARFAPADFIALLDRHGIGWHE
                     KHRGQLFCDRSARQLIDMLLAECAAASAHGGAVERWQPCRVERVEFIAKSGSGAHPKS
                     AASYIIHSERGVVQSPALVVASGGLSIPRIGASGLGLALARQFGLPIVPTRPGLVPLT
                     FDAAAWAPYAPLAGLSLPVRMATGVKAQRIEFQEDLLFTHRGLSGPAVLQISSYWQPG
                     TPITIDLAPGIDLAAALAQAKARSRRWIANELAQWLPARLADTWCARQAAWQRPINEA
                     GDKALAQLAEHLQRWELTPTGTEGYAKAEVMVGGVDTRALSSQTLEAQAQPGLYFIGE
                     VVDVTGWLGGYNFQWAWASAAACADALAAATQ"
     gene            complement(1316..2092)
                     /gene="cobA"
                     /locus_tag="PKN51_01975"
     CDS             complement(1316..2092)
                     /gene="cobA"
                     /locus_tag="PKN51_01975"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNT83897.1"
                     /translation="MSASSTGRVTLVGAGPGDPELLTLKAVRAIQAASVILVDDLVGD
                     GVLAHARPDARITYVGKRGGCKSTPQATIEQLMLQAARAGENVVRLKGGDPFIFGRGG
                     EEVESLRRAGIEPEVINGITAGLAGLTSLGAPLTHREHAQGVVFVTGHPQKDGPGIDW
                     ARLAASARDLRLTLVIYMGISSAARIQAGLLTALPADTPVAVIERASLPAQRQCITRL
                     GELLTELHHSGLGSPAVVVVGDVVRGVAAVHQQPTQLAIA"
     gene            complement(2089..3042)
                     /gene="ybiB"
                     /locus_tag="PKN51_01980"
     CDS             complement(2089..3042)
                     /gene="ybiB"
                     /locus_tag="PKN51_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HNT83898.1"
                     /translation="MSICSYIKVIGRAAPGAQPLEREQAADAMGQILDGTCTDLEIGA
                     FCLAMRIKGETPDELAGFLDAVQARMTRLPASDRPVVVLPSYNGARRLPVLTPLLALM
                     LARKGLPVLLHGSPTEDKRVTSLDVLAALGIGTHAPDERIPSGRVHVVPTERLLPGLK
                     RLLDVRRAIGLRNAGHALVKLLNPIDGPALVVSSYTHPEYAATMASVFDRFDTTALLL
                     RGTEGEPVADARRTPRMVLVSNGQARCVQQAQQGALTTLPRVPTLIDAATTADFSRAV
                     MEGRLPAPSPIVLQVEHIVRACKMLEASYDRTLLQPLVSQI"
     gene            complement(3124..3459)
                     /locus_tag="PKN51_01985"
     CDS             complement(3124..3459)
                     /locus_tag="PKN51_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT83899.1"
                     /translation="MNPALDDPDLRALLRATLAATPAGGAVICLCAAWCGTCRAYEPG
                     FRQAAAAHPALAFRWLDVEDDADALGDLDIETFPTLVIGGADGAVRFAGPVLPQPAQI
                     TRLLHSLGL"
     gene            3516..3818
                     /locus_tag="PKN51_01990"
     CDS             3516..3818
                     /locus_tag="PKN51_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNT83900.1"
                     /translation="MSSIQLHEDPRDLSDATRNMHRAIVSLMEELEAVDWYNQRADVC
                     TDPELKAVLEHNRDEEIEHASMTLEWIRRNNRVFDKELREFLFTDQPLSKAADHEH"
     gene            complement(3948..4934)
                     /locus_tag="PKN51_01995"
     CDS             complement(3948..4934)
                     /locus_tag="PKN51_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNT83901.1"
                     /translation="MVLRGARQALQVERRPLPQPGAGQVRLRVRACAVCRTDLHLQDG
                     DLPMARWPVVPGHEVVGTVDALGAATTGLRLGQRVGVPWLGGACGQCRYCLAGQENLC
                     DAPQFTGCTRDGGFATHIVADASFCVPLDHLALDDASAAPLLCAGLIGWRSLRLAGDD
                     ARVLGLYGFGAAAHLIAQVALAQGRRVHAFTRPGDTAAQQHARALGCTWAGDADQPAP
                     EPLDAAILFAPAGELVPLALRAVRKGGRVVCGGIHMSDIPSFPYALLWQERQLLSVAN
                     LTRADAREFFAALQALPPLRIHTTPYPLQAAQQAMDDLRAGRFQGAAVLLAE"
     gene            complement(4978..5658)
                     /locus_tag="PKN51_02000"
     CDS             complement(4978..5658)
                     /locus_tag="PKN51_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNT83902.1"
                     /translation="MTAALTVISSMATRKLLGELIDKLAGAPVTVESVGGVDAARRVA
                     AGEVFDAVVLGADAIDRLIADGQVRAGSRVDLVRSGVALAVKAGAPRPDIGSAQAVRA
                     AVLAARTVGCSTGPSGVALARLFDHWGIAEQVQARLVVPPPGTPVGALIARGEVELGF
                     QQLSELMFLPGIDVLGPLPEPIQITTVFAGGVPTASRQPQAAQALLERLAAPDLTAIK
                     RANGMDSA"
     gene            5902..6342
                     /locus_tag="PKN51_02005"
     CDS             5902..6342
                     /locus_tag="PKN51_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNT83903.1"
                     /translation="MHTVAEILKSRQGTTLATLPPTATVREALQIMADRNIGSVLIVQ
                     GDALLGIFTERDYARRLVLKGLASADTLLADVMTTRLYVVNPHQTVQECLGVITKGRL
                     RHLPVVDGGKLVGLVSIGDLVNAQLQAQRFMIEQMENYISGNLG"
     gene            6382..7428
                     /gene="tsaD"
                     /locus_tag="PKN51_02010"
     CDS             6382..7428
                     /gene="tsaD"
                     /locus_tag="PKN51_02010"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNT83904.1"
                     /translation="MSSLVLGIESSCDETGVALVRTREHGVPELLAHALHSQVAMHAD
                     YGGVVPELASRDHIRRLIPLANQVLAQAGQALSAIDIVAFTRGPGLAGALLVGAGVAC
                     ALGAALGRPVLGVHHLEGHLLSPFLSQDPPEFPFVALLVSGGHTQLMRVDGVGRYALL
                     GETIDDAAGEAFDKSAKLLGLGYPGGPALARLAEFGDATAFALPRPLLHSGDLDFSFA
                     GLKTAVLTQARKLEHQPCELQRADLAASTQAAIVEVLVKKSLKALTQTGLLRLVVAGG
                     VGANQRLREQLNAACAVRGVRVHYPELALCTDNGAMIALAAAMRVQAGIEAPRSDYAF
                     DVRPRWPLHELAAA"
     gene            7539..8117
                     /locus_tag="PKN51_02015"
     CDS             7539..8117
                     /locus_tag="PKN51_02015"
                     /inference="COORDINATES: protein motif:HMM:NF021515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HNT83905.1"
                     /translation="MWRRWWVGVAPWGLTACVGWPAATAPLHDYQPERFDGAAYVRHF
                     KVDPTRSCEAARRALLSQGYVVTSTQAEQVSGRKYFQPSPEHHVQLEFRVVCASETDD
                     AEAAVVFVSGLREQYIVRKNKESASVGVGGLGSLSLPVEGSMDSMVKVASETVTDAAL
                     YRRFFELLGTYLDRVAVPERRTPQAATPERLS"
     gene            8244..9494
                     /locus_tag="PKN51_02020"
     CDS             8244..9494
                     /locus_tag="PKN51_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT83906.1"
                     /translation="MLSLLGAGSPALAQARTPAVEPIRLALIESLSGPFANTGEAVHR
                     NLVWAVERVNARGGVRTAGGPRPLELLRFDNKGQTEETLAMLRAAIDNGARVVLQGNS
                     SSAAAALIDTIDKHNARDPQRRVLFLNYSAVDPALTNAGCSYWHFRFDAHADMRMTAL
                     MQLLQEDVQLARIYLIGQDYSFGHAVLREARQQLAARRPDVAIVGDELHPVGRVKDFM
                     PYAVKIKASGAQAVLTGNWGNDLTLLVKAARDAGFDGRFYTFYGNALGVPAAIGDAGI
                     GKVVAVADWLPNLPTPESAAFYRAFRQRFPRPQDDYVHLRMQLMIEALAQAIERAGGD
                     DAAMLAQALEQADVTLAGQRGRMRADDHQFQQPLAVGLMERQGAPGVPFDVEGSGYGF
                     RVVRRIDARSAAMPHSCKMARPAH"
     gene            complement(9519..10244)
                     /locus_tag="PKN51_02025"
     CDS             complement(9519..10244)
                     /locus_tag="PKN51_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT83907.1"
                     /translation="MAKTATSPPARRRTRALPVVESPWGPADRRQQQRQTKRNAVLST
                     AAEMFNERGFHATSLDDIAARLHVTKPTLYYYVKNKDEILLECVRKGLHLTLEGIEAS
                     RAAGGRAIDQLRACMLAYGHVVTQPFGMCVIRVGDQEVPVESRIELRRLKSLIDQAFR
                     RLVAAGVEEGSLRPCDPKLTAFTIAGGLSWIGRWYQPGGERSGDEIVQQCVATLMDGV
                     VHRAHTVPPSKPGRRQRPSAASA"
     gene            10377..11240
                     /locus_tag="PKN51_02030"
     CDS             10377..11240
                     /locus_tag="PKN51_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT83908.1"
                     /translation="MATAEPLGSSDTKAPLLAVQDLTLAFGGVRALSGVGFDVQQGSI
                     TAVIGPNGAGKTSLFNTISGFYRPAAGHVRFKGQDITRVPAPLRARMGLGRSFQNIAL
                     FRGMTVLDNIKLGRHAHLKTNVFDALLYLGRARREEAELRRDIEERIIDFLEIDHIRH
                     APVSALSYGLQKRVEMARALAMQPEVLMLDEPVAGMNREETEDMARFILDVRAEWGVT
                     VLMVEHDMGMVMDLSDHVVVLNFGQAIAQGTPAVVQADPEVARAYLGSGDVHELRRKL
                     QAAAVAHQGAS"
     gene            11240..12118
                     /locus_tag="PKN51_02035"
     CDS             11240..12118
                     /locus_tag="PKN51_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT83909.1"
                     /translation="MDWGYLFEVCLTGLAGGGLYALAALAFVLVYKATRVVNLAIGEL
                     LMAGGYLFFTFAVMWALPLWLAIPAAVLASGMLGGLIERLVIRPLLGEPPISAFMVTV
                     GLGSILVGLVEIIWSADQRRLPDFMPSKPIMVGDAFLAPKVFWGAVIAAVFIAAVLLV
                     FRYWRGGVALRATASDQAAAYVVGINVPRVFSLSWVVSAMLAAVSGIIVGSIGGISSS
                     MGVFGLSVLVVVIVGGLDSVLGALVAGLIVGLVEGLAAAYLGGEYKLLATFVVLVSIL
                     MVRPYGLFGTRDIERL"
     gene            12129..13166
                     /locus_tag="PKN51_02040"
     CDS             12129..13166
                     /locus_tag="PKN51_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT83910.1"
                     /translation="MRIGTFKESYVADAALFDSRTQRAWLMVAAAALVLLPFVANDYW
                     LYMACLVAINIASSTGLNLLTGYTGLVSLGQAAFMGLGAYTVAILQIRWGTPLLLNLV
                     AGGAVAMLGGIVVGVPSLRVKGLYLAIVTIAATFIAHFLFANFEFTGGTSGLSMSPGR
                     LFGVELDSSFRLYWLIVPLTILMLLGSANLFRTRIGRAFIAIRDRDISAEVLGIPLLR
                     YKLLSFGLSSFYAGVAGGLWAYFFRVITPESFPLSMSIFFLAAIIVGGMGSTLGSILG
                     AAFMTMVPEVLKLVFDLLPASTDLAVLLSPVRLIIFGALIVGFLIFEPLGLAEMWRRT
                     RRFFHLWPFRN"
     gene            13211..14398
                     /locus_tag="PKN51_02045"
     CDS             13211..14398
                     /locus_tag="PKN51_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT83911.1"
                     /translation="MAQQSFSNRRRSLMLGTAAAGAAALTQPLSVLAQSAEEIVIGGS
                     IPMTGVFAFAGVGINDGIQDYVKIINDAGGIKGRKVKYVPEDTGYKVDVSVAAFKKIT
                     SQNKVSLYYGDSTGFSKTINPELERNGNILMAGASFASELNDPKKYPHQFLVGPDYTE
                     MFGILLKHIAKEKPGAKVAFVYSDSEFGRDPIEKSEAAAKALGLSVPIKIMTAAGSVD
                     VSTEVIKLRRAAPDYTIFHGYILAPIPEFITQGKQQGMTSKWMGTFWTMDSSTVMKMG
                     PDGDGFMGVMPYRYYYDTEKAPMLEKIRAMRPQYQSTAYTQGMLAAMLFLEAAKRCLE
                     GNKPLTGVNLKAALNSIRDFDTGGLIGVPISIPGNSIPVGRVYKADMKAQKMVAASDW
                     IKL"
     gene            14484..15305
                     /locus_tag="PKN51_02050"
     CDS             14484..15305
                     /locus_tag="PKN51_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT83912.1"
                     /translation="MNPRTTDLILEINNIEVVYNKAVQALRGLSLAVPRGQIVALLGS
                     NGAGKSTTLKAVSNLLTLENGALESGSIRFQGQDTARRAPQELVRAGLSHVMEGRHVF
                     EDLTVEENLVAASYALTGRTGAARPDFDLVYHFFPRLHERRKGLAGYLSGGEQQMLAI
                     GRALIAQPQLMLLDEPSLGLSPKLVEEIFGIIVRINAERATSMLLVEQNATVALAVAH
                     SGYIMENGKIVIDGSADRLASDADVREFYLGMGGGGETRSFREIKHYKRRKRWLS"
     gene            15293..17122
                     /locus_tag="PKN51_02055"
     CDS             15293..17122
                     /locus_tag="PKN51_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT83913.1"
                     /translation="MAVLTHAGTLPELTLPQMLRERARRDAQRVAIRQKEFGIWKPYT
                     WAQYYERANHFAQGLVALGMAPGGHLGVISENRIEWVLAQMGASLLGAVTVGVYPTSP
                     TPEVAYVVGHADIDVVVCEDQEQVDKVLEARAQLPRLRRIIMVETKGLRDYPPEQRAL
                     ISSFDDVERLGAQAGHQGLIEDALARQRLDDIGLMIYTSGSTGKPKGAMISWRNMRGV
                     VPGIVDRLALDADASHLSYLPLCHVAEQMLTTFVPIYLGSQVNFGESIRTVQEDLREV
                     APSMFLGVPRIWEKLHASISIKMQETGALRRALFERALRACAPLAQLPRDRWSLAERA
                     RFACAYWLVFRALQNFCGLRKVRVALTGAAPIAPEVVRFFRTLGVPLIEVYGLTESSG
                     MVTGHRLHDVVVGTVGPVTAGVEHRVADNGELQIRGEMVFAGYYKSPEATAQTIVDGW
                     LHTGDVVREEGGQIRIVDRLKDIMITAGGKNLTPSEIENAMKASPYVKECIVIADGRK
                     FVSALVQIEFDTVAKWAEARRIAFTHFRSLVETPEVRQLIEDEVAKGNARLAQVAQVR
                     RFHLLTKELDHDDGEVTATMKVRRASIYKAYAAEIEALYRAAA"
     gene            complement(17104..18201)
                     /locus_tag="PKN51_02060"
     CDS             complement(17104..18201)
                     /locus_tag="PKN51_02060"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNT83914.1"
                     /translation="MALLPYGLSRAVLFNMDPEAAHDLTIDLLARGAGTPLQWTWCHS
                     RIDDPVTLAGLALPNRVGLAAGLDKNARAIDGLAAMGFGFVEVGTVTPLAQPGNPRPR
                     MFRLPAAQALINRMGFNNDGLDAFVRNVRNSRFHSAPRTPDGRQRMILGLNIGKNAAT
                     PIERATDDYLIALAGVYPFADYVTVNISSPNTKNLRDLQSDAALDALLSSLKSRQGEL
                     AAEHGRQVPLFLKIAPDLDGEQVQAIADALRRHGIDGVIATNTTLSREAVAGLPHADE
                     AGGLSGAPVREASNRVIGELRAALGAGYPIFGVGGIMSGADAVAKIEAGADAVQIYTG
                     LIYRGPGLVGEVARALRRSRAAGAPAQAAAR"
     gene            18247..18927
                     /gene="rpiA"
                     /locus_tag="PKN51_02065"
     CDS             18247..18927
                     /gene="rpiA"
                     /locus_tag="PKN51_02065"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNT83915.1"
                     /translation="MTQDELKAMVGRAALEYVEAGQVVGVGTGSTVNHFIDALASMKE
                     RIPGAVSSSEASTARLQALGIPVFDCNAVGEIAVYIDGADEIDPRGHMIKGGGAALTR
                     EKIVAAQSRRFVCIADDSKRVEVLGRFPLPVEVIPMAAQRIVRQFAALGGQAELRHKD
                     GAPLVTDNGQHILDVRGLRIGDPLAFESTVNQWPGVVTVGVFAHQKAQICLLGTSQGV
                     RTLRFDGA"
     gene            19118..19657
                     /locus_tag="PKN51_02070"
     CDS             19118..19657
                     /locus_tag="PKN51_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83916.1"
                     /translation="MPGPTERRLLTLALNVMLAVAILAAGVLAWRFAGGPPPDGPPGV
                     RVARLPPGSFVWASAPDDLRYLPDGLRAQDAGRLALLLLRERDGTLRAFYLPRQGGRA
                     SVPAAASPAVAGIPCEDMAPDFGRGDIACRQTSAGFDFAARHRWSLQGRALSPGTPDL
                     YAVPGQELDGDWLPQALRR"
     gene            19743..20639
                     /locus_tag="PKN51_02075"
     CDS             19743..20639
                     /locus_tag="PKN51_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT83917.1"
                     /translation="MQRLDFGPVRVYLGEKSGKYPDGNQVIVQGRDTRVAFDTPQVAR
                     HIGPDLDGVDLVLLGHVHEDHMVALGQLRHAAVQVHEADLAAAQSWPGLARHYGYPAP
                     VLEAMRAVIARDFSYHPRPDATAYTHGSQWDLGGGVRVRAHHLPGHTAGHSALVIESE
                     GIAFIGDIDLTGFGPYYGDASSDLRQFQRSLAAVRGLDARLWVTSHHRGVITERAAFE
                     QALARFAARIDERHERLLGYLSEGPQTLDQLVARRLLYPIGIELPYVDSAERRSIQMH
                     LDLLIEQCLVQAVGEGGYALAS"
     gene            complement(20691..20781)
                     /locus_tag="PKN51_02080"
     tRNA            complement(20691..20781)
                     /locus_tag="PKN51_02080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20745..20747),aa:Ser,seq:gga)
     gene            complement(20840..21760)
                     /locus_tag="PKN51_02085"
     CDS             complement(20840..21760)
                     /locus_tag="PKN51_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNT83918.1"
                     /translation="MEIALIVVIIAALFIKLAIKVVPQQNAWVVERLGKYHGTLAPGL
                     NFIMPFLDRVAYRHSLKEIPMDVPSQVCITRDNTQLQVDGILYFQVTDPMRASYGASN
                     YVVAITQLAQTTLRSVIGKLELDKTFEERDMINAKVVEAIDEAALNWGVKVLRYEIKD
                     LTPPAEILRSMQAQITAEREKRAVIATSEGKRQEEINLAEGQKQAAIAKSEGDKQAAI
                     NTAQGQAAAITEVANATAVALARIGTAIRQPGGEQAVQLKVAERAVEAYSQLASQAQT
                     TLVVPSNMTEVSALISSAMKMVQTQQRSEG"
     gene            complement(21809..22234)
                     /locus_tag="PKN51_02090"
     CDS             complement(21809..22234)
                     /locus_tag="PKN51_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNT83919.1"
                     /translation="MADSTVWWIAAGLLVAAELLTGTFYLLMLAIGAAAGALAAHAGL
                     SLSAQLVAGGVTGGVAVLVSYLARRRRPGDPSPRADRSVNLDVGETVQIDAWGADGTA
                     QIRYRGAPWTAVLRPGHTPHPGPHRVIELVGSRLLVEPA"
     gene            22370..24664
                     /locus_tag="PKN51_02095"
     CDS             22370..24664
                     /locus_tag="PKN51_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HNT83920.1"
                     /translation="MKFRFPIVIIDEDYRADNISGLGIRALAKAIEDEGFEVVGATSY
                     GDLSQFAQQQSRASAFILSIDDEELGADEASSPAVQNLRSFIAEVRRKNDDVPIYLHG
                     ETKTSQHLPNDVLRELHGFIHMFEDTPEFVAKHIVREAKSYLEGIQPPFFRALLDYAE
                     DGSYSWHCPGHSGGVAFLKSPIGQMYHQFYGENMLRADVCNAVEELGQLLDHNGAIGD
                     SERNAARIFNADHCFFVTNGTSTSNKIVWHHTVAPGDVVVVDRNCHKSNLHAIIMTGA
                     IPVFLKPTRNHYGIIGPIPRSEFEPETIKAKIRANPLLQHVDADRVKPRILTLTQSTY
                     DGVLYDTEAIKGMLDGYVDNLHFDEAWLPHAAFHPFYGSYHAMGKKRARPQQSVVYST
                     QSIHKLLAGISQASHVLVQDSQQVKLDRYLFNEAYLMHTSTSPQYSIIASCDVAAAMM
                     EPPGGTAMVEESIAEALDFRRAMRKVEADFGKGDWWFKVWGPEKIAEEGIGRADDWVI
                     KNGAKDRSWHGFGKLAPGFNMLDPIKATIVTPGLNLDGKFDKSGIPASIVTKFLAEHG
                     VVVEKTGLYSFFIMFTIGITKGRWNTLVTALQQFKDDYDRNQPMWRIMPEFCAQHRRY
                     EKMGLRDLCQHVHQLYAKYDIARLTTDMYLSELTPAMNPADAYACIARRQVERVEIDQ
                     LEGRITTALVTPYPPGIPLLIPGEVFNRKIVDYLKFARDFALECPGFETDIHGLVEVE
                     DDHGHVRYFADCVAADARGVRGGL"
     gene            24693..24923
                     /locus_tag="PKN51_02100"
     CDS             24693..24923
                     /locus_tag="PKN51_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010351400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HNT83921.1"
                     /translation="MFPEYRDLITRLKGEGSNTRFLNLFERHNELDHQIKAMEGHDAG
                     AIHSEIETLKKEKLRVKDELYTILRQEATVGK"
     gene            complement(25037..25648)
                     /locus_tag="PKN51_02105"
     CDS             complement(25037..25648)
                     /locus_tag="PKN51_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNT83922.1"
                     /translation="MSALLLAGGVQAQVYRSSGPGGVSYSDQPPPAQAGRQVAPLGGD
                     TRRSGSATLPYTLSQTVQRFPVTLYTSADCPPCVSGRNLLIQRGVPFSEKTVQSNEDI
                     AALQRLAGASQVPVLTIGGQRLSGYSDQEWSQYLDAAGYPRTSQLPAAYQRPAATPLV
                     AQSPVPAAATGTAREATAPAAATPAEPSIAPPRNATNPAGIRF"
     gene            complement(25744..27774)
                     /locus_tag="PKN51_02110"
     CDS             complement(25744..27774)
                     /locus_tag="PKN51_02110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNT83923.1"
                     /translation="MTNPLLDFSDLPRFDQIRPEHVAPAIDQLLAEAEAALEAAVSPD
                     SPADWTWLSRTLDVPGERLGRAWSVVSHLNAVMDTPELRAAYNAALPRVTEFWTRLGS
                     DERLYARYKAIDPAPLDAAQRRARDNALRDFVLGGAELQGAAKQRYAAIQERLAELSQ
                     KFSENALDATDAYACYASEAEVEGLPPDVLATARAAAQADGQSGYKLTLKLPSYLPAM
                     QFAHDRALRQRLYRAYVTRASDQAEGDATRFDNAPLIREILALRHEEAQLLGYASYGD
                     VSLVPKMAESPRQVIDFLRQLATKARPYAERDVADLRAFARERLDLADPQSWDWPYLG
                     ERLKEARYAFSEQEVKQYFQAPKVLAGLFKIVETLFEVAIRRDAAPVWHPSVEFYRIE
                     RDGQLLGQFYLDPGARTGKRGGAWMDDVRARWLRPDNGRLQTPVAQLVCNFAEGVHGA
                     PPLLTHDDVITLFHEFGHGLHHLLTQVNERDVSGISGVEWDAVELPSQFMENFCWEWE
                     VLRHMTAHVDSGEPLPRELFDRMIAARNYQAGLQTVRQIEFALFDMLLHTEHASGQDF
                     QPLLDAVRREVAVLQPPPFARTAQTFSHIFAGGYAAGYYSYKWAEVLSADAYAAFEES
                     VTPEGLPSVETGRKYRREILEAGGSRPAMESFKAFRGREPSLDALLRHQGMA"
     gene            complement(27919..28773)
                     /locus_tag="PKN51_02115"
     CDS             complement(27919..28773)
                     /locus_tag="PKN51_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HNT83924.1"
                     /translation="MTAQLIDGNALAATVRAEVAHRVQALQARGVQPHLSIILVGDNP
                     ASQVYVRSKVADSQQTGLSATLERYPAELPQAELLARIAALNADPAVHGILVQLPLPP
                     HMDAHAVIEAIAPAKDVDGFHVASAGALMVGQPGFRPCTPYGCMRMLQSIGLHDLRGR
                     HAVVIGRSNIVGKPMAMMLLTQNATVTVCHSGTSDLAALTRQADIVVAAVGKRNVLTA
                     DMVKPGAVVIDVGMNRDDAGKLCGDVDFTGVRQVASWITPVPGGVGPMTRAMLLVNTI
                     EAAERAAA"
     gene            complement(28856..29482)
                     /locus_tag="PKN51_02120"
     CDS             complement(28856..29482)
                     /locus_tag="PKN51_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT83925.1"
                     /translation="MSLIPKRGTVYVVDDDEAVRDSVQWLLEGQDYRVRCFESAEAFL
                     ARYDPREVACLIIDIRMDGMSGLELQDRLNERHSPLPMAFITGHGDVPLAVDTMKKGA
                     MDFIQKPFNEDHLMPLVERMLEQARTSFAEHQQAASRDALLAKLTGREAQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLGANTVADLLKIALGQQTAKV"
     gene            complement(29479..32001)
                     /locus_tag="PKN51_02125"
     CDS             complement(29479..32001)
                     /locus_tag="PKN51_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT83926.1"
                     /translation="MQSELASTLYDSGPPRTRWQRLLKGQDPIAQLAPLAAVLLFLAA
                     IVSAFWYLRLEETRREQEAVKRDVEYSQQRLRLRLLERQEQLMRLARELADREMTALQ
                     FDARAEAMVAQYPELRAVSWIDGQRRLRTTQTSPSVSPQPDWIADGRLKPGEVSEGYS
                     LAQDLRQAVYNQPLAVEDADAVLYLHVPITPRAQFAGVVLAEYSVDGLLRYGVPSEVL
                     AKYAVSLLDAKGQLLAGQTIPDRTGLSEYLPWSEQVNAFDSPVSPVGNGLVLRAQAWR
                     TSQGVVGGTLFWLVAVLASMTAWMLIANWRQSQRRQQAQHALLAETSFRRAMENSMLT
                     GMRALDLQGRIIYVNPAFCQMTGWSEEELLGQAEPFSYWPDADRAELSSRLKEELRGT
                     ADPAGFQMSVKRKDGSLFDARLYVSPLIDATGKHAGWMASMTDITEPNRVRRQLSASY
                     ERFTTVLEALDASVSVAPLGSQELLFANKLYRQWFDGTTAGHLKLVERAGTPETQASE
                     ESLDTVDSLVGLPVDSLANAPLAEHAEVYIERLNKWLEVRSRYLNWVDGRLVQMVIAT
                     DITARRQAEEQAAAQAERAEAASRLITMGEMASSVAHELNQPLTAISNYCTGMISRVR
                     SGQISRDDLLGALDKTARQAQRAGQVVQHIRSFVKRSEPRRAWAEVTPMVEQVLELAE
                     IELRRRNVRLVRQIATDLPALHVDPILIEQVLMNLIKNAAESIDVAQRAPGQRLIELR
                     VVQRDIEDQPTVQFSVTDNGRGLAPEALARLYEAFFSTKSDGLGIGLNLCRSIVESHQ
                     GRIHAENLYNAGQVVGCRFSFWIPVGPAQDARPSDHNLREVA"
     gene            32230..34926
                     /gene="aceE"
                     /locus_tag="PKN51_02130"
     CDS             32230..34926
                     /gene="aceE"
                     /locus_tag="PKN51_02130"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNT83927.1"
                     /translation="MSNNPQAQSAAELDAQETREWLDALTAVIEREGPERAHALLEGL
                     LEHARQASIDLPFSANTGYVNTLEPEQEARCPGNIAIEKRLRAYMRWNAMAMVVRANR
                     IDPPEGGSLGGHIGSFASLASMWGAGFNHFWHAESEHHGGDCIYFQGHSAPGIYARAY
                     LEGRINEEQLENFRQEVKGKGLSSYPHPHLMPGFWQYSTVSMGLGPMMAIYQARFMKY
                     LHARGIANTEGRKVWVFLGDGEMDEPESRGAITLASRENLDNLIFVINCNLQRLDGPV
                     RGNGKIIQELEGDFRGAGWNVIKLLWGKGWDDLLRRDKDGKLRQLMMETLDGDYQTFR
                     AKDGAYIRQHFFGRYPETAKLVEHLTDDEIWDLRRGGHDPEKVYAAFHAANAHQGEPT
                     VLLVKTVKGYGMGRAGEGRNTVHQTKLLDDEDIRYIRDRFNIPIPDSELDKLPYYKPA
                     DDTPEMKYLHERRQALGGYLPHRREKADESFTIPSLETFKAVIEPTAEGREISTTQAY
                     VRFLTQLLRDKALGPRVVPILVDEARTFGMEGLFRQIGIYNPKGQLYTPQDREQVMYY
                     KESVDGQILQEGITEAGGMCSWIAAATSYSTNNRIMIPFYIYYSMFGFQRFGDLAWAA
                     GDMLARGFILGGTSGRTTLNGEGLQHEDGHSHILAGTIPHCLTYDPSFAHEVAVILHD
                     GLKRMVEKQENVFYYITLLNENYAMPGLTPGTEQQILQGMYLCKPGAEGERRVQLLGS
                     GSILRESLEAQKLLATDWGVQADVWSCPSFNQLTREGQDVERWNLLHPLEQPRVPFVA
                     RQLAGHPGPVVASTDYLKNYAEQIRPFVPKDRPYIVLGTDGFGRSDFRARLREHFEVD
                     RHYIAVAALKGLADAGALPRQKVADAIRKYGIDTEKINPLHA"
     gene            34939..36576
                     /gene="aceF"
                     /locus_tag="PKN51_02135"
     CDS             34939..36576
                     /gene="aceF"
                     /locus_tag="PKN51_02135"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HNT83928.1"
                     /translation="MALVEVKVPDIGDFSDVAVIELLVAPGDTVKLEQSLITVESDKA
                     SMEIPSSAAGVVRELKVKIGDKVSQGSLVLLLESADSASKPAPAQVQQAPVAIDTIAN
                     AAPPAAASVASGPVEVRVPDIGDFKDVAVIEVFVQPGDTIRVEQSLITVESDKASMEI
                     PSSAAGVVREMKVKVGDKVNQGDLLALLEGAAPVAAAPAAAAPANAPAPAAVSAPAAP
                     PLAAAPAHEPTRAPLGLPHASPSVRKFARELGVPLAEVKGSGPKGRITQEDVQAFTRA
                     VMSGAQRTQAQGAAGVSGGGVTGGGELNLIPWPKVDFAKFGPIERKELSRIKKISGAN
                     LMRNAVMIPAVTNHDDADITDLEAFRVATNKENEKSGVKVTMLAFLIKACVAALKKFP
                     EFNSSLDGDALVYKNYWHIGFAADTPNGLVVPVIKDADKKGVLQISTEMSELAKKARD
                     GKLGPADMSGATFTISSLGGIGGRYFTPIINAPEVAILGVCRSNIEPVWNGEKFKPRL
                     MLPLSLTWDHRVIDGAAAARFNAYLGQILADFRRVLL"
     gene            36590..38434
                     /gene="lpdA"
                     /locus_tag="PKN51_02140"
     CDS             36590..38434
                     /gene="lpdA"
                     /locus_tag="PKN51_02140"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNT83929.1"
                     /translation="MAVIDIKVPDIGDFADVGVIELLVKPGDTVKVEQSLITVESDKA
                     SMEIPSSHAGVVKELKVKLGDKVSEGSVILTLEAAGASATSPAGAGEGRDEGGVQAGA
                     QPSPSPQPPPASGRGREPAPIASSFAGTADLDCDVLVLGGGPGGYSAAFRAADLGLKV
                     VLVERYATLGGVCLNVGCIPSKALLHVAAVMDEVGHLAALGVDFGKPKLDIDKLRSHK
                     EKVIAKLTGGLGAMAKMRKVTLVRGYGSFVGANHLQVEETTGSGQDKTGSKRVVQFKR
                     AIIAAGSQAVRLPFMPEDPRVVDSTGALALKAVPKKMLILGGGIIGLEMGTVYSTLGA
                     RLDVVEMLDGLMQGADRDLVKVWQKMNAPRFDHIMLKTKTVGAKATDKGIEVVFEGEG
                     APAPQTYDLVLQAVGRSPNGKKIGADQAGVAVTERGFIDVDIQMRTNVPHIFAIGDIV
                     GQPMLAHKAVHEAHVAAEVIAGELKGDEALARAAFNARVIPSVAYTDPEVAWVGLTED
                     QAKAQGIKVKKGLFPWSASGRAIANGRDEGYTKLLFDEATHRIVGGGIVGTHAGDMIG
                     EVALAIEMGADAVDIGKTIHPHPTLGESIGMAAEVAHGSCTDVPPQRK"
     gene            38851..39462
                     /locus_tag="PKN51_02145"
     CDS             38851..39462
                     /locus_tag="PKN51_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNT83930.1"
                     /translation="MAWHTWLIYFVAALGLSLSPGPNGLLALTHGALHGRRKTLFTVA
                     GGALGFATVIALSMFGIGALLQASLVWLTVLKWVGGGYLVWLGVQVWRAPPIAALALQ
                     PQGEVNGWSLFRQGALSALTNPKGILFFAAFLPQFIDPARSLLLQYFIMAGTFVAIEF
                     ITEVLIASMAQRISGWLARVGRGFNRVCGGIFAAIGVALPLRA"
     gene            complement(39624..40460)
                     /locus_tag="PKN51_02150"
     CDS             complement(39624..40460)
                     /locus_tag="PKN51_02150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNT83931.1"
                     /translation="MSTISPSAVTALVPAYQAAEFIQPTLDALSAQTHPALHVLVSVD
                     LCDDDTQAICERHALRDPRFRVVRQTSRLGWVGNSNFLLSQAQGDYALFAFHDDLLAP
                     TYIARLAAELDADPQACVAFSDVLLTDTQGGQEFWSYPTLDGERSAFIRGVRVLERRG
                     KWWVPNRGLFRLSMARQIGGLKKHGAGEFSADWPWVLHLALLGSFVRVPELLCHKFYK
                     PGSLSRSWDFSQPQYFEVHAACMREIWNSSLGTQEKIALALPLMQWLERHRHLAMTPP
                     ST"
     gene            complement(40457..41773)
                     /locus_tag="PKN51_02155"
     CDS             complement(40457..41773)
                     /locus_tag="PKN51_02155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT83932.1"
                     /translation="MSRSVPDSRVWVVGAGLQGCAIALELAHRGQPVVLLDQDALPMN
                     RASLRNEGKIHLGLIYAADASGATARVQLRGALVFQHLLERWLGRPVAAALPLSTPFA
                     YLVARDSVLPAPTLARHFDRVQALYKHWMTQHPEASYLGQRPTRLAWPASRDEHQGLV
                     LAPGESVFTTAERAIDTDALAAALRRAVITHPLIDWLPGRRITRFAPAPDGVTLHGQV
                     LGSGGEPLEPLEACARQVVNATWEQRLGLDASMGLQPAPGWLHRLKYRVIVRLPAGWE
                     HLSSVTMVLGRYGDVVMRPNGTAFVSWYPEGLRGWSEALVPPPEWDGPCRGDVSPDQA
                     QALAEAILRAADRWYPGLHRATVLNVDAGAIFAYGREDVDRLDSGLHARSFNGPRGDA
                     RYLSVDSGKLTTAPWYAVQAADLLLGQRTALAQTWTDVDPDGAAAP"
     gene            complement(41880..43052)
                     /locus_tag="PKN51_02160"
     CDS             complement(41880..43052)
                     /locus_tag="PKN51_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT83933.1"
                     /translation="MNSERTPAVPSRLPNVGTTIFTVMSALATQHQAVNLGQGFPDFD
                     CDPALVDAVTAAMRQGLNQYPPMPGVPALREAIAAKIEALYGRHYDAGAEITVTAGAT
                     QAILTAILCCAGPGDEVIVLEPCYDSYVPNIELSGARVVRVPLTPGSFRPDFDRLAAA
                     LSPRTRAILINSPHNPSGMVWSAQDMLRLQELLAPTDALVISDEVYEHMVYAPLRHES
                     AARYPGLAARSFIVSSFGKTCHVTGWKVGYVAAPAVLTAEFRKVHQFNVFTVNTPMQY
                     GIAAYMQDPRPYLELPAFYAAKRDLFRAGLAQTRFRLLPCEGTYFQCAAIDELSVPER
                     DLSEADFCQWLTREIGVAAIPLSAFYGDGFDQKVIRFCFAKQDGTLDNALDRLARL"
     gene            complement(43042..43983)
                     /locus_tag="PKN51_02165"
     CDS             complement(43042..43983)
                     /locus_tag="PKN51_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HNT83934.1"
                     /translation="MNLHQFRFVQEAARRNLNLTEAAKALHTSQPGVSKAIIELEEEL
                     GIDIFTRHGKRLKRITEPGQHVLDSIAIILREVGNLKRIGEQYSAQDTGTLSIATTHT
                     QARYVLPRPVARLREQYPKVNISLHQGAPDQVARMVIEETAEIGVATESLASYPELVT
                     LPCYEWQHVLVLPATHPLSKKERLALEDIAAQPLITYHPSFTGRTRVDAAFAQRQLTP
                     RVVLEAIDSDVIKTYVRLGLGIGIVAEMAMADDRQGDLVARPLGPLLGQNVARIALKR
                     GAWLRDFVYHFASLLSDRLDKDLILKAMSGHLNDYEL"
     gene            complement(44027..44410)
                     /locus_tag="PKN51_02170"
     CDS             complement(44027..44410)
                     /locus_tag="PKN51_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNT83935.1"
                     /translation="MQGPDRHGIVLFAHGSREAAWRAPIEAVAERIRQLAPGRPVACA
                     YLEHTAPDLPTAVDRLLAGGADAVTVWPMFLGVGRHAREDLPRLLQVLRTQHPQVRFS
                     LQPAISEHPEVLAAMAHAMLDTPSS"
     gene            complement(44413..45519)
                     /gene="lptG"
                     /locus_tag="PKN51_02175"
     CDS             complement(44413..45519)
                     /gene="lptG"
                     /locus_tag="PKN51_02175"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNT83936.1"
                     /translation="MNTIRHLIYREVLRAVGFVALAFLALFFFFDLVDALGQLERGAG
                     RGYHAGHALLNVALQVPSHLYELLPIAVLIGTIYVMAQLAASSQFTILRTSGLGPLRA
                     LGMLLVLALGFVALTFVCGDYVAPEANRAVQNLQARFLGDITVGRTGAWLKERRDDSQ
                     YAVNVGTMSADGTLNQVQIFQFSIADGRLLTMTKASRGHFSDQGWTLQAVEQRNFEQV
                     PQAEPRVQVQRLDEWVWPNALSQDTVAAALLNPNRMQTLDLFRYIRHLQSNQQSAQRY
                     EIEFWRKVFYPLSCLVMVVLALPFAYLHFRTGNITGYVFVGVLVGISFFLLNNVFGFI
                     GNLRNWEPWLAAAAPGVIYSVLSLAAFGWLVLRQ"
     gene            complement(45516..46613)
                     /gene="lptF"
                     /locus_tag="PKN51_02180"
     CDS             complement(45516..46613)
                     /gene="lptF"
                     /locus_tag="PKN51_02180"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNT83937.1"
                     /translation="MLFDSSIRKELGRAFSATLVVLVTVVMTVLLIRTLGQASAGRVN
                     PSEVMLVMGYTVLGHLATILTLSLFVAITATLTRMYAASEMVVWFASGQGLAAFLRPV
                     LRFAWPVLLVVAVLALAVWPWANHQIRELRDRYQARGDIERVAPGRFIESAGGQRVFF
                     IDKDRADAETGHNIFIATNEHGKETVTTAQRGRLEVIKGERFVVLDHGHRLELDSATG
                     QTRVSEFAEYGNRISTRLVGPTANDSPTMKPTLTLLSQPTPRHQGELSWRIGLILAAL
                     NCAVFALAATRVNPRVGRSAGLVFALFAFTTYYNLLTFGEGWIARGRVGLVAYLLLLH
                     GGVLLLSLGWLLARHHGWGSAWRRRLNGGGA"
     gene            46637..48175
                     /locus_tag="PKN51_02185"
     CDS             46637..48175
                     /locus_tag="PKN51_02185"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNT83938.1"
                     /translation="MDFELQSLRLADAAARKSPALMVLVADGFAGGEDALSALIARTV
                     ASGDFKTEAGAQLFVYGAAGIVPERVVLAGIGDGSPARVRKAVGTAMGALRGGPAKAV
                     TLCFAQPAEADALRAAVLALADASYTYSATKSKPNDKKEPPRIGKAVLGVPEPGPLRE
                     ALQQAVAVARGVELAREWANRPGNHATPTLLADAARQLGKLPRIGCEVLGPKEVARLG
                     MGAFQAVAQGSRQELRFIVLRYDGAPRSQAPVVLVGKGITFDTGGISIKPAPEMDEMK
                     FDMSGAASVLGVFRALAELRPALNVVGLIPACENMPDGGAIKPGDVVTSMSGQTIEIL
                     NTDAEGRLVLCDALTYAERFKPCAVIDIATLTGACVIALGGVRSGMFASDDALAQALQ
                     SAGEQALDPCWRMPLDDEYGEALKSNFADVGNVGGRPAGAVTAAKFLQRFADKFSWAH
                     LDIAGTAWKGGSAKGATGRPVGLLVHYLLMQAGRPVKAGGEPTKRAKTKGQAGARAGK
                     GGKA"
     gene            48202..48633
                     /locus_tag="PKN51_02190"
     CDS             48202..48633
                     /locus_tag="PKN51_02190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNT83939.1"
                     /translation="MTAVAFHFNVPDKLGYACRLLRKAYAAGGPVGVVAPSGMLSELD
                     TRLWAFSALDFVPHCLADAPASMRAVTPIVLAEDCAQLPPATAVLVNLGPQVPDGFER
                     FGRVIELVTGDDDDRAQARQRWRHYAGRGLEIQRHDLAAGA"
     gene            48637..49038
                     /locus_tag="PKN51_02195"
     CDS             48637..49038
                     /locus_tag="PKN51_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83940.1"
                     /translation="MNPDDEPSARPPPRGVPTLTEVVQAAAPPSEAPAPASLPQPDSP
                     VSPTSHVALEPAVPAQDPVDALLERLGPELERQIAEAVGRVLHEQMLGFNSRVRKAVA
                     DVVRDTVAASLLQAGGRSDVSENPENQPSDT"
     gene            49134..>49493
                     /locus_tag="PKN51_02200"
     CDS             49134..>49493
                     /locus_tag="PKN51_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT83941.1"
                     /translation="MQVQLKLTVAAALALVAGLASAQEQVVKIASVAPMSGGQAHYGK
                     DNGNGVTMAIEDLNKQNITIGGKKIKWEAVIEDDAADPKQGAAVAQKLCDMKVNGVVG
                     HLNSGTTIPASKIYHECG"
BASE COUNT         7935 a        16274 c        17317 g         7967 t
ORIGIN      
        1 cttttgccag caaagcccgc aattataggt tattgcgttg cagcggcaag cgcgtcggcg
       61 caggcggcgg cgctggccca ggcccactgg aagttgtagc cgcccagcca gccggtcacg
      121 tcgaccacct cgccgatgaa gtacaggccc ggctgggctt gcgcctccag cgtttgcgac
      181 gacagcgccc gggtgtcgac gccgcccacc atgacctcgg ccttggcata gccttcggtg
      241 ccggtgggcg tcagctccca gcgctgcagg tgttcggcca gttgcgccag cgccttgtcg
      301 ccggcctcgt tgatggggcg ctgccaggcc gcttgccggg cgcaccaggt atcggccagg
      361 cgggccggca gccactgcgc cagctcgttg gcgatccacc ggcgcgaacg cgccttggcc
      421 tgcgccaggg cggcggccag gtcgatgccg ggcgccagat cgatggtgat gggggtgccg
      481 ggctgccagt agctggagat ctgcagcacc gccgggcccg acaggccgcg gtgggtgaac
      541 agcaggtctt cctggaattc gatgcgctgc gccttgacgc cggtggccat gcgcaccggc
      601 agcgacagcc cggccagcgg cgcgtagggc gcccaggcgg cggcgtcgaa ggtcagcggc
      661 accagccccg ggcgggtcgg cacgatgggc agcccgaact ggcgcgccag cgccaggccc
      721 aggccgctgg cgccgatgcg ggggatcgac agcccgccgc tggcgaccac cagcgccggg
      781 ctttgcacga ccccgcgttc actatgaata atgtagcttg cggcgctttt tgggtgcgcg
      841 ccagaacctg atttggctat gaattcgacg cgctcgacgc ggcacggctg ccagcgctcg
      901 accgcgccgc cgtgggctga ggcggcggcg cattcggcca gcagcatgtc gatcagctgg
      961 cgcgccgagc ggtcgcagaa cagctggccg cggtgcttct cgtgccagcc gatgccgtgc
     1021 cgatcgagca gggcgatgaa gtctgccggc gcaaaacgcg ccagcgccga gcgacagaag
     1081 cgcgggttgg cgctcaggta gtggcgttgc ggcgccttgg ggtcgaggtc gcggttggtg
     1141 aagttggcgc gcccgccgcc ggagatgcgc accttttcgg ccagccgcgc ggcatggtcg
     1201 atcagcagca cacgcagccc gcgctggcca gcgcgcgcgg cgcagaacag gccggcggcg
     1261 ccggcgccca ggatgacggc gtcgaaggcg cccgccgcag ccatgccggg gctgctcagg
     1321 cgatggcgag ctgggtcggc tgctggtgga cggcggccac gccgcgcacc acgtcgccca
     1381 ccaccaccac ggccgggctg cccaggccgc tgtggtgcag ctcggtgagc agctcgccca
     1441 ggcgggtgat gcactggcgc tgggcgggca ggctggcgcg ctcgatcacg gccaccgggg
     1501 tgtcggcggg caaggctgtc agcagcccgg cctgaatgcg cgcggcgctg ctgatgccca
     1561 tgtagatcac cagcgtcagg cgcaggtcgc gcgccgatgc ggccagtcgc gcccaatcga
     1621 tgcccgggcc gtccttctgc gggtggccgg tgacgaacac cacaccctgc gcgtgctcgc
     1681 ggtgggtgag tggcgcgccc aggctggtca gcccggccag gccggcggtg atgccgttga
     1741 tcacctctgg ctcgatgccg gcgcggcgca gactttcgac ctcttcgccg ccgcgaccga
     1801 agatgaaggg atcgccgccc ttcagacgca ccacgttttc gccggcgcgc gcggcctgca
     1861 gcatcagctg ctcgatggtg gcctggggcg tgctcttgca gccgccgcgc ttgccgacat
     1921 aggtgatgcg ggcgtcgggc cgggcgtgcg ccagcacgcc gtcacccacc aggtcgtcga
     1981 ccaggatcac gctggcggcc tggatggcgc gcacggcctt gagtgtcagc agttcggggt
     2041 cgcccgggcc ggcgcccacg agcgtgacgc ggccagtgga agaagcactc atatctggga
     2101 caccaggggt tgcagcagcg tccgatcgta cgatgcttcg agcatcttgc aggcgcgcac
     2161 gatgtgttcg acctgcaaga cgatggggct gggcgccggc aggcggcctt ccatcacggc
     2221 gcggctgaag tcggcggtgg ttgcggcgtc gatcagcgtc ggcacccgcg gcagcgtggt
     2281 cagcgcaccc tgctgcgctt gctgcacgca gcgcgcctgg ccgttgctga ccagcaccat
     2341 gcgtggcgtg cggcgtgcgt cggccaccgg ttcgccctcg gtgccgcgca gcagcagggc
     2401 ggtggtgtcg aagcggtcga acaccgaggc catggtggcg gcgtattcgg ggtgggtgta
     2461 gctggatacc accagggccg gcccgtcgat cgggttgagc agcttgacca gggcgtgccc
     2521 ggcgttgcgc aggccgatgg cgcgccgcac gtccagcagg cgcttgaggc cgggcagcag
     2581 gcgctcggtg ggcaccacat gcacccgccc gctggggatg cgctcgtcgg gggcgtgggt
     2641 gccgatgccc agggcggcca gcacgtccag gctggtcacg cgcttgtctt cggtcgggct
     2701 gccgtgcagc agcaccggca ggcccttgcg cgccagcatc agcgccagca gcggcgtgag
     2761 caccggcagc cggcgcgccc cgttgtagct gggcagcacc accaccgggc ggtcgctggc
     2821 gggcaggcgg gtcatgcgcg cctgcacggc gtccaggaat ccggccagct catcgggcgt
     2881 ttcacccttg atgcgcatgg ccaggcagaa ggcgccgatt tcgaggtcgg tgcaggtgcc
     2941 atcgaggatc tggcccatcg cgtcggccgc ctgctcgcgt tccagcggct gggcgccggg
     3001 ggcggcgcga ccgatgacct tgatgtacga acagatggac atgggaggca ttgtgcggcg
     3061 cgggcggcca ggactcaatt gaccatggtc aaggaattga tcggcctgcg cgggccgtcg
     3121 gactcacagg cccaggctgt gcagcagccg tgtgatctgt gccggctggg gcagcaccgg
     3181 cccggcgaag cgcacggcgc cgtcggcgcc accgatgacc agggtgggga aggtttcgat
     3241 gtccagatcg cccagcgcgt cggcgtcgtc ctccacgtcg agccagcgga acgccagcgc
     3301 gggatgcgcc gccgccgcct gccggaagcc gggctcgtac gcgcggcagg tgccgcacca
     3361 ggcggcgcac aggcagatca ccgcaccgcc ggccggggtg gctgccagcg tggcacgcag
     3421 cagggcgcgc aggtcgggat cgtccagggc cgggttcatc gggttacagt ggggcgcaca
     3481 agcattgtcc cacccgaccg aggagagtca gcgccatgtc gagcatccag ttgcacgaag
     3541 acccccgtga tctgtccgac gccacccgca acatgcaccg cgccatcgtt tcgctgatgg
     3601 aggagctgga agcggtggac tggtacaacc agcgcgccga cgtctgcacc gaccccgaat
     3661 tgaaggcggt gctggagcac aaccgcgacg aggagatcga gcacgccagc atgacgctgg
     3721 agtggatccg tcggaacaac cgcgtgttcg acaaggaact gcgggaattc ctgttcaccg
     3781 atcagccgct cagcaaggcg gccgaccacg agcactagac tgagtgtcgc ccatcgaacc
     3841 ctggcaagct ataaaaacaa gagctgctgg cgcttgatac aagcgcgcca gagccgaatt
     3901 gtttgtaaga tcttggcggc aggcggtgtc cgcttgcgcc ggcatcgtca ctccgccagc
     3961 agcaccgccg ctccctgaaa tcgcccggcc cgcaggtcgt ccatggcttg ctgggcggcc
     4021 tgcagcgggt agggcgtggt gtgtatgcgc agcggcggca gggcctgcag tgcggcaaag
     4081 aactcgcgtg cgtcggcgcg cgtcaggttg gccaccgaca gcagttgccg ctcctgccac
     4141 agcagggcat agggaaagct ggggatgtcg ctcatgtgga tgccgccgca caccacgcgt
     4201 ccgcccttgc gcaccgcccg caacgccagc ggcaccaact cgcctgccgg ggcaaacagg
     4261 atggccgcgt ccagcggctc gggggcgggc tggtcggcgt cgccggccca ggtgcagccc
     4321 agggcgcgtg cgtgctgctg cgccgccgtg tcgccggggc gggtgaaggc gtgtacgcgc
     4381 cgaccctgcg ccagcgccac ctgggcgatc agatgggcgg cggcgccgaa gccgtacagg
     4441 cccagcacgc gcgcatcgtc accggccagg cgcagcgagc gccagccgat cagaccggca
     4501 cacagcagcg gggcggcgct ggcgtcgtcc agcgccaggt ggtcgagcgg cacgcagaag
     4561 ctggcgtcgg ccacgatgtg ggtggcaaag ccgccgtcgc gcgtgcagcc ggtgaactgg
     4621 ggcgcgtcgc acaggttttc ctgccccgcc aggcagtagc ggcactggcc gcaggcgccg
     4681 cccagccagg gcacgccgac gcgctggccc aggcgcagtc cggtggtggc ggcgcccagg
     4741 gcgtcgacgg tgccgaccac ttcgtggccg ggcaccaccg gccagcgcgc catcggcagg
     4801 tcaccatcct gcaggtgcag gtcggtgcgg cagacggcgc atgcgcgcac ccgcaggcgc
     4861 acctggccgg cgccgggctg gggcagcggg cggcgctcga cctgcagcgc ctggcgcgcg
     4921 ccgcgcaaca ccatggccag gctggtttcg gtgggttcgt tcatgcgggg ctgtgggtca
     4981 ggcgctgtcc atgccgttgg cgcgcttgat ggcggtcaag tcgggcgctg ccagccgctc
     5041 cagcagcgcc tgtgcggcct gcggctggcg cgacgcggtg ggcacgccgc cggcaaatac
     5101 ggtggtgatc tggatcggtt cgggcagcgg gccgagcacg tcgatgccgg gcaggaacat
     5161 cagttcgctg agctgctgga aacccagttc cacctcgccg cgggcgatca gcgcgcccac
     5221 cggcgtgccg ggcggcggca ccaccagccg ggcctgcacc tgctcggcga tgccccagtg
     5281 gtcgaacagc cgggccagcg ccacgccgct gggtccggtg gaacagccca cggtgcgcgc
     5341 cgccagcacg gcggcccgca ccgcctgggc gctgccgatg tcgggccgtg gcgcgccggc
     5401 ttttaccgcc agcgccacgc cggagcgcac caggtcgacc cggctgccgg cgcgcacctg
     5461 gccgtccgcg atgagccggt cgatggcgtc ggcacccagc accacggcat cgaacacctc
     5521 gccggcggcc acgcgtcgcg ccgcgtcgac gccgccgacc gactccaccg tcaccggcgc
     5581 gccggccagc ttgtcgatca actcgcccag cagcttgcgg gtggccatgg aggaaatcac
     5641 ggtgagggcg gcggtcatgt cacgcggggg tcggggaaat caaatgaatc ggccatcaag
     5701 gcacgatcat caagcgccat aagctatcaa atacatagcg catgcgggca cagtcaggac
     5761 cattgtgccg ccaaatccta gcccgatgtg gcgccgccgc ggcgtcggca cagaatttga
     5821 cgcaggccct tcccaactgc gccaaagtcg gcaaaatgac cgtatcgacg tgttcatccc
     5881 agtctttgca ggagaagcgc catgcatacc gttgccgaga ttctgaaatc gcgccagggc
     5941 acgacactgg caacgctgcc gcccaccgcc accgtacgcg aggcgctgca gatcatggcc
     6001 gaccgcaaca tcggctcggt gctcatcgtg cagggtgacg cgctgctggg catcttcacc
     6061 gagcgcgact acgcgcgccg cctggtgctc aagggtctgg cctcggccga caccctgctg
     6121 gccgacgtga tgaccacgcg cctgtacgtg gtcaacccgc accagacggt gcaggaatgc
     6181 ctgggcgtga tcaccaaagg gcggctgcgc catctgccgg tggtcgacgg cggcaagctg
     6241 gtgggcctgg tgtcgatcgg cgatctggtc aatgcccaac tgcaggcgca gcgcttcatg
     6301 atcgagcaga tggagaacta catctccggc aacctgggtt gagcgcgcgt tgccgccaca
     6361 gggcgcggat aatgccgcgc catgtcttca ctggttctgg gcatcgagtc ctcgtgcgac
     6421 gaaaccggcg tggctctggt gcgcacgcgc gagcatggtg tgccggagct gctggcgcac
     6481 gcgctgcaca gccaggtcgc catgcacgcc gactatggcg gggtggtgcc ggagctggcc
     6541 agccgagacc acatccggcg cctgattccc ttggcaaatc aggttctggc acaggcaggt
     6601 caagcgctgt cagctattga tatagtagca ttcacacgtg gcccgggcct tgccggtgcc
     6661 ttgctggtgg gggccggggt ggcctgtgcc ctgggtgcgg cgctggggcg accggtgttg
     6721 ggggtgcacc atctggaagg gcatctgctg tcgccttttc tgagccagga tccgccggaa
     6781 tttcctttcg tcgccctgct ggtcagcggc ggccacacgc agctgatgcg ggtcgacggg
     6841 gtggggcgct acgccctgct gggcgaaacc atcgacgacg ccgctggcga agccttcgac
     6901 aagtcggcca agctgctcgg tctgggctat ccgggcgggc cggcgctggc gcggctggcc
     6961 gagttcggtg acgccacggc gttcgccctg ccgcgtcccc tgctgcacag cggcgatctg
     7021 gatttttcgt ttgccggtct gaaaaccgcg gtgctgacgc aggcgcgcaa gctggagcac
     7081 cagccgtgcg agctgcagcg cgccgatctg gccgccagca cgcaggcggc gatcgtcgag
     7141 gtgctggtga aaaagtcgct caaggcgctg acccagaccg ggctgctgcg cctggtggtg
     7201 gccggcggcg tgggcgccaa ccagcggctg cgtgaacagc tgaacgcggc ctgcgctgtg
     7261 cgcggggtgc gtgtgcacta ccccgaactg gcgctgtgca ccgacaacgg cgccatgatc
     7321 gcgctggcgg cggccatgcg cgtgcaggcc ggcatcgagg cgccgcgcag cgactatgcg
     7381 ttcgatgtgc ggccgcgctg gccgctgcac gagctggcgg cggcctgatg cgccagcggg
     7441 gcgacttttc ttttcccgcg cggggtcgtc gctgccctgc cgggcgttag cattcacggt
     7501 ttttcgtcag gcggtcgttt cggatgtctt gggtggtgat gtggcgtcga tggtgggtgg
     7561 gggtggcccc gtgggggctg acggcatgcg tcggctggcc ggccgccacg gcgcccttgc
     7621 acgactacca gcccgagcgc tttgacggcg ccgcctatgt gcgccacttc aaggtcgacc
     7681 ccacacgttc gtgcgaggcg gcgcggcgcg ccctgctgag ccagggctac gtggtgacca
     7741 gcacgcaggc cgagcaggtg tcgggccgca agtacttcca gcccagtccc gaacaccatg
     7801 tgcagctcga atttcgcgtg gtgtgtgcca gcgagaccga cgacgccgag gcggccgtgg
     7861 tgttcgtcag cgggctgcgc gagcagtaca tcgtgcgcaa gaacaaggaa tcggcctcgg
     7921 tcggcgtggg cggcctgggc tcgctgtcgc tgccggtcga aggcagcatg gattcgatgg
     7981 tcaaggtggc cagcgaaacc gtcaccgacg ctgccctgta ccggcgcttc ttcgaactgc
     8041 tgggcaccta cctggaccgc gtggccgtgc ccgagcggcg cacgccgcag gcggcgacgc
     8101 ccgaacgcct gtcctgattg cggctttcct gccggcgtcg tccggcctgc tccggccaac
     8161 gacggccgtg tgtgttcttc tgttgttctt gactccttct cttcatgtca catctcttgc
     8221 accgtcgcgc cgccctgggc gccatgctgt ccctgctcgg cgccggctcg cccgccctgg
     8281 cgcaggcccg tacacccgct gtcgaaccga tccgactggc cttgatcgag tcgctcagcg
     8341 gcccgtttgc caacaccgga gaagcggtgc atcgcaacct ggtctgggcg gtcgagcgcg
     8401 tcaacgcccg cggcggcgtg cgcaccgcgg gcgggccgag gccgctggag ctgctgcgct
     8461 ttgacaacaa ggggcagacc gaggagacgc tggccatgct gcgcgccgcc atcgacaacg
     8521 gcgcccgcgt ggtgctgcag ggcaactctt ccagcgcggc ggcggcgctg atcgacacca
     8581 tcgacaagca caacgcgcgc gatccccagc ggcgggtgct gtttctgaac tattcggcgg
     8641 tcgatccggc gctcaccaat gccggctgca gctactggca ctttcgcttc gacgcccacg
     8701 ccgacatgcg catgacggcg ctgatgcagt tgctgcagga agatgtgcag ctggcgcgca
     8761 tctacctgat cggccaggac tacagcttcg gccacgccgt gctgcgcgag gcgcgccagc
     8821 aactggcggc gcggcggccc gacgtggcca tcgtcggtga cgagctgcat cccgtcggcc
     8881 gggtgaagga cttcatgcct tatgccgtga agatcaaggc ctccggcgcg caggcggtgc
     8941 tgaccggcaa ctggggcaac gacctgacgc tgctggtcaa ggcggcgcgc gacgccggtt
     9001 ttgacgggcg cttctacacc ttctacggca acgcattggg cgtgccggcg gccatcggcg
     9061 atgccggcat cggcaaggtg gtggcggtgg ccgactggct gcccaacttg cccacgcccg
     9121 agagcgccgc gttctaccgc gcgttccgcc agcgctttcc ccgcccgcag gacgactacg
     9181 tgcacctgcg catgcaactg atgatcgagg cgctggcgca ggccatcgag cgcgccggcg
     9241 gcgacgatgc cgccatgctg gcgcaggcgc tggagcaggc cgacgtcacg ctggccggtc
     9301 agcgcgggcg catgcgcgcc gacgatcacc agttccagca gccgctggcc gtgggcctga
     9361 tggagcgcca gggtgcgccc ggcgtgccgt tcgacgtcga aggctcgggc tacggctttc
     9421 gtgtggtgcg ccgcatcgac gcgcgcagcg ccgccatgcc gcacagctgc aagatggcgc
     9481 ggccggcgca ctgagtcttt cctgaagtct ggttcgcgtc aggcggaagc ggcggagggc
     9541 cgctgtcggc ggccgggttt gctcggtggc acggtgtgcg cgcgatggac gacaccgtcc
     9601 atcagcgtcg ccacgcactg ctggacgatc tcatcgcccg aacgttcgcc gcccggttgg
     9661 taccagcggc cgatccaact caagccgccg gcgatggtga atgcggtcag cttggggtcg
     9721 caggggcgca ggctaccttc ttcgaccccg gcggcgacca ggcgccggaa cgcctgatcg
     9781 atgagggact tcaggcggcg cagctcgatc cggctttcga ccggcacctc ctggtcaccg
     9841 acgcggatca cgcacatgcc gaagggctgc gtgacgacgt gaccgtaggc cagcatgcag
     9901 gcgcgcagtt ggtcgatcgc ccgtccgccg gcggcgcgcg atgcctcgat gccctccagc
     9961 gtcaggtgca ggcccttgcg cacgcattcg agcaggatct cgtccttgtt cttgacatag
    10021 tagtagagcg tcggtttggt cacatgcagg cgggctgcga tgtcgtcgag cgaagtggca
    10081 tgaaagccac gttcgttgaa catttcggcc gcggtggaca gcaccgcgtt gcgtttggtc
    10141 tggcgctgct gctggcgccg gtcggccggg ccccagggcg attcgacgac aggcagggcg
    10201 cgcgtgcgtc ggcgcgcggg aggggaggtt gcagtcttcg ccatgtctgc gtgctgagat
    10261 tcatcgggaa aacaccgatc ccggttggcc ggcatccacc ggtatattta ctcttgagta
    10321 actagtctac ggtcaagtag agatcgtggg atacgaatta accccaacac agcatcatgg
    10381 cgacggcgga gccacttggc agctcagata cgaaggcgcc tctgcttgcg gtgcaagacc
    10441 tgacgctggc cttcggtggc gtgcgggcgc tgtcgggtgt ggggtttgac gtccagcagg
    10501 gcagcatcac ggccgtgatc ggtccgaacg gtgccggcaa gacgtcgctg ttcaacacca
    10561 tttcgggctt ctaccggcca gccgcgggtc atgttcggtt caagggccag gacatcacgc
    10621 gcgttcctgc gccgctgcgc gcccgcatgg gactggggcg cagctttcag aacatcgcac
    10681 tgtttcgcgg catgacggtg ctcgacaaca tcaagctcgg gcgccacgcg cacctcaaga
    10741 cgaatgtgtt cgacgcgctg ctctacctgg gccgtgcgcg ccgcgaggag gccgagctgc
    10801 gccgcgacat cgaggagcgc atcatcgact tcctcgagat cgaccacatc cgccacgccc
    10861 ccgtctcggc cctgtcctac ggcctgcaaa agcgcgtgga gatggcgcgc gccctggcca
    10921 tgcagcccga ggtgctgatg ctcgacgagc ccgtggccgg catgaaccgc gaggagaccg
    10981 aggacatggc gcgcttcatc ctcgacgtgc gcgccgaatg gggtgtgacg gtgctcatgg
    11041 tggagcacga catgggcatg gtgatggacc tgtcggacca tgtggtggtg ctcaacttcg
    11101 gccaggcgat cgcccagggc acgccggcgg tggtgcaggc cgaccccgag gtggcgcgcg
    11161 cctacctggg ctcgggcgac gtccatgaac tgcgtcgcaa gctgcaggcg gcggcagtcg
    11221 cacaccaggg ggcgagctga tggactgggg ctacctgttc gaggtctgtc tgaccggtct
    11281 ggccggtggc ggcctgtatg cgctcgcggc gctggccttc gtcctggtct acaaggcgac
    11341 gcgggtcgtg aacctggcca tcggcgagtt gttgatggcg ggcggctacc tgttcttcac
    11401 cttcgcagtg atgtgggcgt tgccgctgtg gctggcgatt ccggcggcgg tactggccag
    11461 tggcatgctg ggcggcctga tcgagcggct ggtgatccgg cccctgctgg gcgagcctcc
    11521 gatttcggcg ttcatggtca ccgtcggtct gggctcgatc ctggtgggcc tggtggaaat
    11581 catctggagc gccgatcagc gccggctgcc ggacttcatg cccagcaagc ccatcatggt
    11641 gggcgacgcg ttcctcgcgc ccaaggtgtt ctggggcgcg gtgatcgccg cggtgttcat
    11701 tgccgcggtg ctgctggtgt ttcgctactg gcgcggtggc gtggccctgc gcgccacggc
    11761 aagcgaccag gccgcggcgt acgtggtggg catcaacgtg ccgcgggtgt tttcgctgtc
    11821 ctgggtggtg tcggccatgc tggcggcggt gtccgggatc atcgtcggct cgatcggtgg
    11881 catttcgtcg tccatgggcg tgtttggtct gtcggtgctg gtggtggtga tcgtcggcgg
    11941 gttggacagc gtgctcggcg ccctggtggc ggggttgatc gtcggtctgg tcgagggtct
    12001 ggcggcggcc tacctgggcg gtgagtacaa actgctggcg accttcgtgg tgctggtgag
    12061 cattctcatg gtgcgtccct acggcctgtt cggcacgcgg gacatcgaaa ggctgtaggc
    12121 ccgtcgtcat gcgcatcgga accttcaagg aaagctacgt cgccgacgcg gcgctgttcg
    12181 actcgcgcac gcagcgcgcg tggctgatgg tggccgccgc ggcgctggtg ctgcttccgt
    12241 tcgtggccaa cgactactgg ctgtacatgg cctgcctggt cgccatcaac attgccagca
    12301 gcaccgggct gaacctgctg acgggctata ccggtctggt cagcctgggg caggcggcgt
    12361 tcatgggcct gggggcctat acggtggcca ttctgcagat ccgctggggt acgccgctgc
    12421 tgctcaacct ggtggcgggc ggtgcggtgg ccatgctggg cggcatcgtg gtcggcgtgc
    12481 cgtcgctgcg cgtcaagggc ctgtacctgg cgatcgtcac catcgccgcc accttcatcg
    12541 cccactttct gttcgccaac ttcgagttca cgggtggcac cagcgggttg tccatgtcgc
    12601 ccgggcggtt gttcggtgtc gaactggaca gctcgtttcg cctgtactgg ctgatcgtgc
    12661 cgctgacgat cctcatgctg ctgggttcgg ccaacctgtt tcgcacgcgc atcggccgcg
    12721 ccttcatcgc catccgcgac cgcgacatct cggccgaggt gctgggcatt ccgctgttgc
    12781 gctacaagct gctgtcgttc gggctgtctt cgttctacgc cggtgtggcg ggcgggttgt
    12841 gggcgtattt ctttcgcgtc atcacgcccg agagctttcc gctgtcgatg tctattttct
    12901 tcctggccgc catcatcgtc ggtggcatgg gttcgacgct gggcagtatt ctgggcgcgg
    12961 ccttcatgac gatggtgccg gaagtgctca agctggtgtt cgacctgctg cccgccagca
    13021 ccgacctggc ggtgctgctg tcgccggtgc gcctgatcat ctttggcgcc ctgatcgtcg
    13081 gcttcctgat cttcgagccg ctgggtctgg ccgagatgtg gcgccgcaca cgacggttct
    13141 ttcacctgtg gcccttccgc aactgagcgc gggtcatttt ttcgcaacgt cctgcttcaa
    13201 ggagacattc atggctcagc aatccttttc caaccgtcgc cgcagcctga tgctcggcac
    13261 ggccgcggcg ggcgccgccg cactgacgca gccgctgtcg gtgctggccc agagcgccga
    13321 ggagatcgtc atcggcggct cgattccgat gaccggtgtg ttcgcctttg ccggcgtggg
    13381 catcaacgac ggcatccagg actacgtgaa gatcatcaac gacgccggcg gcatcaaggg
    13441 ccgcaaggtc aagtacgtgc ccgaggacac cggttacaag gtggacgtgt cggtggcggc
    13501 cttcaagaag atcaccagcc agaacaaggt cagcctgtac tacggcgatt cgaccggctt
    13561 ttccaagacc atcaacccgg agctggagcg caacggcaac attctgatgg ccggcgcgtc
    13621 gttcgccagc gagctcaacg accccaagaa atacccgcac cagttcctgg tcgggccgga
    13681 ctacaccgag atgttcggca tcctgctcaa gcacatcgcc aaggaaaagc cgggcgccaa
    13741 ggtggccttc gtgtactcgg actcggagtt cggccgcgac ccgatcgaaa agagcgaggc
    13801 cgcggccaag gccctgggtc tgtcggtgcc gatcaagatc atgaccgccg ccggcagcgt
    13861 ggacgtgtcc accgaagtca tcaagctgcg ccgcgccgcg cccgactaca cgattttcca
    13921 cggctacatc ctggcaccca tccccgagtt catcacccag ggcaagcagc aggggatgac
    13981 cagcaaatgg atgggcacct tctggaccat ggacagctcc accgtcatga agatgggccc
    14041 ggacggagac ggcttcatgg gcgtcatgcc ctaccgctac tactacgaca ccgagaaggc
    14101 gccgatgctg gagaagatcc gtgccatgcg gccgcagtac cagagcaccg cctacacgca
    14161 gggcatgctg gccgccatgc tgttcctgga ggcggccaag cgctgcctgg aaggcaacaa
    14221 gcccctgacg ggcgtcaacc tgaaagcggc gctgaactcg attcgcgatt tcgataccgg
    14281 cggcctgatc ggcgtgccga tcagcatccc gggcaactcg attccggtcg gtcgcgtcta
    14341 caaggccgac atgaaggcac agaagatggt ggccgcatcc gactggatca agctgtgagt
    14401 ctgacccgga acgccgaccc ggcgtgctcc cgcccggtcg gcggggccag ggttggaaca
    14461 cgtcgaggcc aggggtgacc cccatgaacc cgcgcacgac cgacctgatc ctcgaaatca
    14521 acaacatcga ggtggtctac aacaaggcgg tgcaggccct gcgcggcctg tcgctggccg
    14581 tgccgcgcgg gcaaatcgtg gcgctgctgg gcagcaacgg cgccggcaag agcaccacgc
    14641 tgaaggccgt gtccaacctg ctgacgctgg agaacggggc gctggagtcg ggcagcatcc
    14701 gtttccaggg tcaggacacg gcgcgccggg cgccgcagga gctggtgcgt gccggcctgt
    14761 cgcacgtgat ggaagggcgc cacgtgttcg aggatctgac ggtggaggaa aacctcgtcg
    14821 ctgccagcta cgcgttgacc gggcgcaccg gtgcggcccg gccggatttc gatctcgtct
    14881 accacttctt tccgcgcctg cacgagcggc gcaagggtct ggcaggctat ctgtccggcg
    14941 gcgagcagca gatgctggcg atcgggcgcg cgctgatcgc ccagccccag ctgatgctgc
    15001 tggacgagcc ttcgctgggc ctgtcgccca agctggtgga agagatcttc ggcatcatcg
    15061 tgcgcatcaa cgccgagcgg gcgacctcca tgctgctggt cgagcagaac gccaccgtgg
    15121 cgctggccgt ggcgcacagc ggctacatca tggaaaacgg caagatcgtg atcgatggca
    15181 gcgccgatcg cctggccagc gacgccgacg tgcgcgagtt ctacctgggc atgggcggcg
    15241 gcggcgaaac ccggagcttt cgcgagatca aacactacaa gcggcgcaag agatggctgt
    15301 cctgacccac gctggcacct tgcccgaact gaccctgccg cagatgctgc gcgagcgcgc
    15361 ccggcgcgac gcacagcgcg tggcgatccg gcaaaaggaa ttcggcatct ggaagcccta
    15421 cacctgggca cagtattacg agcgcgcgaa ccatttcgcg cagggtctgg tggcgctggg
    15481 catggcacca ggcgggcacc tgggcgtgat ttcggagaac cgcatcgaat gggtgctggc
    15541 acagatgggt gccagcctgc tgggtgccgt cacggtcggc gtctatccca ccagcccgac
    15601 gccggaggtg gcctacgtgg tggggcacgc cgacatcgat gtcgtggtct gcgaggacca
    15661 ggagcaggtg gacaaggtgc tggaggcgag ggcgcagctg ccgcgcctgc gcaggatcat
    15721 catggtcgaa accaagggcc tgcgcgacta cccccccgag cagcgcgcgc tgatctccag
    15781 cttcgacgac gtggagcggc tgggtgcgca ggccggccac cagggcctga tcgaggacgc
    15841 actggcgcgc cagcggctgg acgacatcgg cctgatgatt tacacctcgg ggtccaccgg
    15901 caagcccaag ggcgccatga tctcgtggcg caacatgcgg ggcgtggtgc ccgggatcgt
    15961 cgatcggctg gcgctcgacg cggacgcttc acacctgtcc tacctgcccc tgtgccacgt
    16021 cgctgagcag atgctgacca ccttcgtgcc gatctacctg ggctcgcagg tgaacttcgg
    16081 cgagtcgatc cgcaccgtgc aggaagacct gcgcgaagtg gcgcccagca tgttcctggg
    16141 ggtgccgcgc atctgggaga aactgcacgc ctcgatttcg atcaagatgc aggaaaccgg
    16201 cgcgctgcgc cgcgcactgt tcgagcgcgc ccttagggcc tgcgcgccgc tggcgcaact
    16261 gccccgggat cggtggtcgc tggccgagcg ggcgcgcttt gcctgtgcgt actggctggt
    16321 gtttcgtgca ctgcagaact tctgtggtct gcgcaaggtg cgggtggcgc tcacgggggc
    16381 ggcgccgatc gcgcccgaag tggtgcgctt ctttcgcacg ctgggggtgc cgctgatcga
    16441 agtctacggt ctgaccgaat cctccggcat ggtcaccggc catcgtttgc acgacgtggt
    16501 ggtgggcacc gtcgggccgg tcaccgcggg ggtcgaacac cgggtggccg acaacggcga
    16561 gctgcagatt cgcggtgaga tggtgtttgc cggctactac aagagccctg aagccacggc
    16621 gcagaccatc gtcgatggct ggctgcacac cggtgacgtg gtgcgcgagg agggcggtca
    16681 gatcaggatc gtcgaccgcc tgaaggacat catgatcact gcgggtggca agaacctgac
    16741 gccgtcggaa atcgaaaacg cgatgaaggc cagcccctac gtcaaggagt gcatcgtcat
    16801 cgccgatggt cgcaagttcg tcagcgcgct ggtgcagatc gagttcgaca ccgttgccaa
    16861 atgggccgag gcgcgtcgca tcgccttcac gcatttccgt tcgctggtcg agacgcccga
    16921 agtgcgtcag ctgatcgaag acgaggtggc gaagggcaat gcccgcctgg cccaggtggc
    16981 acaggttcgg cgcttccacc tcctgacaaa ggaactcgac cacgacgacg gcgaagtcac
    17041 cgccaccatg aaggtgcgcc gcgccagcat ctacaaggcc tacgcggccg agatcgaggc
    17101 gctctatcgc gctgccgcct gagcgggggc gcccgcagcg cggctgcggc gcagcgcccg
    17161 cgccacttcg cccaccaggc caggcccccg gtagatcagc ccggtgtaga tctgcaccgc
    17221 gtcggcgccg gcttcgatct tggccacggc gtcggcaccg ctcatgatgc cgcccacgcc
    17281 gaagatcggg tagccggcgc ccagcgccgc gcgcagctcg cctatgacgc ggttgctggc
    17341 ttcgcgcacc ggcgcgcccg acaggccgcc ggcctcgtcg gcatggggca agccagccac
    17401 ggcctcgcgc gacagcgtgg tgttggtggc aatcacgccg tcgatgccgt ggcggcgcag
    17461 tgcgtcggcg atggcctgca cctgctcgcc gtccagatcg ggcgcgatct tcaggaacag
    17521 cggcacctgc cgcccatgct cggccgccag ttcgccctgg cgcgacttga gggacgacag
    17581 cagcgcgtcc agcgcggcgt cgctctgcag atcgcgcagg ttcttggtgt tggggctgga
    17641 gatgttgacc gtgacgtagt cggcaaacgg gtagacgccg gccagggcga tcaggtagtc
    17701 gtcggtggcg cgctcgatcg gggtggccgc gttcttgcca atgttcaggc caagaatcat
    17761 gcgttggcgc ccgtctggcg tgcgcggagc gctatgaaaa cgggagtttc tgacgttgcg
    17821 cacaaaggcg tccagcccgt cgttgttgaa gcccatgcgg ttgatcaggg cctgcgcggc
    17881 gggcaggcgg aacatgcgcg gtctggggtt gccgggctgt gccagcgggg tgacggtgcc
    17941 gacttcgaca aagccgaagc ccatcgccgc cagaccgtcg atggcccgcg cgttcttgtc
    18001 cagcccggcg gccaggccca cgcggttggg cagggcgagg ccggccagcg tcaccgggtc
    18061 gtcgatgcgg ctgtggcacc aggtccactg cagcggggtg ccggcaccgc gcgccagcag
    18121 gtcgatggtg aggtcgtgcg cggcctcggg gtccatgttg aacaggacgg cgcgcgacag
    18181 gccgtagggc agcagggcca ttggataatt cttgcttgaa acgggaacag agggaaattg
    18241 tcgcaaatga ctcaggacga actcaaagcc atggtcgggc gcgccgcgct cgagtacgtc
    18301 gaggcgggcc aggtggtagg cgtgggcaca ggttcgacgg tgaaccactt catcgacgcg
    18361 ctggcgagca tgaaggaacg catccccggc gccgtgtcct cgtcggaagc gtcgactgcg
    18421 cgcctgcagg ccctgggcat tccggtgttc gactgcaacg ccgtgggcga gatcgccgtc
    18481 tatatcgacg gcgccgacga gatcgacccg cgtggccaca tgatcaaggg cggcggcgca
    18541 gcgctgacgc gcgagaagat cgtggccgcc cagtcgcgcc gcttcgtctg catcgccgac
    18601 gacagcaagc gcgtggaggt gctggggcgt tttccactgc cggtggaagt gattccgatg
    18661 gcggcgcagc gcatcgtgcg ccagtttgcc gccctgggcg gccaggccga actgcgccac
    18721 aaagacggcg ccccgctggt gaccgacaac ggccagcaca tcctggacgt gcgcggcctg
    18781 cgcatcggcg atccgctggc gttcgaaagc accgtcaacc aatggccggg cgtggtcacc
    18841 gtgggcgtgt tcgcgcacca gaaggcgcag atctgcctgc tgggcacgtc ccagggcgtg
    18901 cggacgctgc gctttgatgg tgcctgagtg cggcaactgg tcgctcgcca tcttcgcgac
    18961 agacgcatga ccgccgggtt tcgtcgcctc gatccccatc accggccggg tgagcatgcc
    19021 gcgtccttgc ggcttgcccg ccaccccgga cacggcaccg gtgggcgcgt ccaacggcca
    19081 cgcgacggct gagcggcccg ccgtcgatcg acacaccatg cccggcccaa ccgagcggcg
    19141 tctgttgacg ctggcactga acgtcatgct ggccgtggcg attctggcgg ccggggtgct
    19201 ggcgtggcgg tttgctggcg ggccgccgcc cgatggcccg cctggcgtgc gcgtggcgcg
    19261 cctgccgccc ggcagcttcg tctgggccag cgcgcccgat gacctgcgct atctgcccga
    19321 cggcctgcgc gcccaggacg ccggccgtct ggcgctgctg ctgctgcgcg agcgcgacgg
    19381 caccctgcgc gccttctatc tgccacgcca gggtgggcgc gccagtgtgc cggcggccgc
    19441 ctcgccagcg gtggccggca ttccgtgcga ggatatggcc ccggacttcg ggcgtggcga
    19501 catcgcctgt cggcagacca gcgccggttt tgacttcgcg gcgcgccatc gctggtcgct
    19561 gcaaggtcgg gccctcagcc caggcacgcc cgatctgtac gcggtgccgg ggcaggaact
    19621 ggacggcgac tggctgccac aagcgctgcg ccgctgagcc atggggtatc ccatgggtgt
    19681 gcaatggaaa atgcctccag cgcttgctgg gtgtgcgcaa cgagctattc aatttgtagc
    19741 ttatgcaacg tctggatttt gggcccgtgc gggtctatct gggtgaaaaa tcgggcaagt
    19801 accctgacgg aaaccaggtc atcgtgcagg gccgcgatac ccgcgtggcg ttcgacacgc
    19861 cgcaggtggc gcgccacatc gggccagacc tggacggggt ggatctggtg ctgctgggcc
    19921 atgtgcacga ggaccacatg gtggccctgg gccagctgcg ccacgccgcc gtgcaagtgc
    19981 atgaggccga cctggcggcc gcgcagtcgt ggccaggcct ggcgcgccac tacggctatc
    20041 cggcgcccgt actcgaggcg atgcgtgccg tgatcgcgcg cgatttcagc taccacccgc
    20101 gccccgacgc caccgcttac acccacggca gccagtggga tctggggggc ggcgtgcgcg
    20161 tgcgcgccca tcacctgccg ggccacaccg ccggccattc ggcgctggtg attgaaagcg
    20221 agggcatcgc cttcatcggc gacatcgacc tgaccggctt cggcccctac tacggcgacg
    20281 ccagctccga cctgcgccag tttcagcgca gtctggcggc ggtgcgcggg ctcgacgcgc
    20341 gcctgtgggt cacctcgcac caccgtggcg tgatcaccga gcgtgccgcc ttcgaacagg
    20401 ccttggcacg ttttgcggcc aggatcgacg agcgccatga gcgcctgctg ggctacctga
    20461 gcgaagggcc gcagacactc gatcagctcg tcgcgcgtcg cctgctgtac ccgatcggga
    20521 tcgagctgcc ctacgtcgac agcgccgaac gccgcagcat ccagatgcac ctcgacctgc
    20581 tgatcgagca gtgtctggta caagcggtgg gcgagggtgg gtacgccctg gcgagctgaa
    20641 cgacggcgca aaaaagagcg gcccgtggat cgggccgctc atgtctgtcc tggcggagag
    20701 ggcgggattc gaacccgcgg tgggctatta acccacacac gctttccagg cgtgcgactt
    20761 aaaccgctca tccacctctc cgtgcaatcg cgcattgtag cgcggtgcag gcgcgcctgg
    20821 tgcctgcgtg caagtgcgtt cagccttcgc tgcgctgctg cgtctgcacc atcttcatgg
    20881 ccgagctgat gagggccgag acttcagtca tgttgctggg cacgaccagg gtggtctggg
    20941 cctggctggc gagctggctg taggcctcga cggcgcgttc ggccaccttc agttgcaccg
    21001 cctgctcgcc tccaggctgg cggatggcgg tgccgatgcg cgccagcgcc acggcggtgg
    21061 cgttggccac ctcggtgatg gcggcggcct ggccctgggc ggtgttgatg gcggcttgct
    21121 tgtcaccttc ggatttggcg atggcggcct gcttctggcc ttcggccagg ttgatttctt
    21181 cctgtcgttt accttccgag gtggcgatga cggcgcgctt ttcgcgctcg gccgtgatct
    21241 gtgcctgcat ggaacgcagg atttcggccg gcggcgtgag gtccttgatt tcgtagcgca
    21301 gcaccttcac gccccagttc agcgccgctt cgtcgatggc ctcgaccacc ttggcgttga
    21361 tcatgtcgcg ctcttcgaag gtcttgtcca gctccagctt gccgatcacg ctgcgcagcg
    21421 tggtctgcgc cagctgggtg atggcgacca cgtagttgct ggcgccgtag ctggcgcgca
    21481 tcgggtcggt gacctggaag tacaggatgc cgtccacctg cagctgcgtg ttgtcgcggg
    21541 tgatgcagac ctggctgggc acgtccatcg ggatttcctt caggctgtga cgataggcca
    21601 cgcgatccag gaagggcatg atgaagttca ggcccggtgc cagcgtgccg tggtacttgc
    21661 ccagccgctc gaccacccag gcgttctgct ggggcacgac cttgatcgcc agcttgatga
    21721 acagcgcggc gatgatgacg acgatgagtg cgatttccac gatggcttct ttcgttgagg
    21781 ttcggtgggc ggaggtggcg tggcgccgtc aggccggctc gaccagcagg cggctgccca
    21841 ccagctcgat cacgcggtgc gggcccgggt ggggcgtgtg gccgggtcgc agcacggcgg
    21901 tccagggcgc gccgcggtag cggatctggg ccgtgccgtc ggcgccccag gcgtcgattt
    21961 gcacggtttc accgacgtcg aggttgacgc tgcggtcggc gcgcggcgac gggtctcccg
    22021 gccggcgccg gcgcgccagg tagctgacca gcaccgccac gccgccggtc acgccgccgg
    22081 cgaccagctg ggcgctcagg ctcagcccgg cgtgggccgc cagcgcgccg gccgcagcgc
    22141 cgatggccag catcagcagg tagaaggtgc cggtcagcag ctcggccgcc accagcagcc
    22201 cggcggcgat ccaccagacg gtcgagtctg ccatgcatgc tcccagttaa tgatttcgat
    22261 gtatcactga tgcgcattgt cgggcatctg cacgcggctg cggcgccggg gcgcggcaat
    22321 tccgtacact tgcgcgctgt tttgccgcct tcggcaggag tcaggtccca tgaagttccg
    22381 ctttccgatc gtcatcatcg acgaagacta ccgcgccgac aacatctccg gcctgggcat
    22441 ccgtgccctg gccaaggcga tcgaggacga gggtttcgag gtggtgggtg ccaccagcta
    22501 tggcgacctg agccagttcg cccagcagca aagccgcgcc agtgccttca tcctgtcgat
    22561 cgacgacgag gagctggggg ccgacgaggc atcgtccccc gcggtgcaga acctgcgcag
    22621 cttcatcgcc gaggtgcggc gcaagaacga cgacgtgccg atctacctgc acggcgagac
    22681 caagaccagc cagcacctgc ccaacgacgt gctgcgcgag ctgcacggct tcatccacat
    22741 gttcgaggac acgcccgagt tcgtggccaa gcacatcgtg cgcgaggcca agagctatct
    22801 ggaaggcatc cagccgccgt tctttcgcgc attgctcgac tatgccgagg atggttcgta
    22861 ctcctggcac tgtccggggc attcgggcgg ggtggcgttc ctgaagtcac cgatcggcca
    22921 gatgtaccac cagttctacg gcgagaacat gcttcgcgcc gacgtctgca acgccgtcga
    22981 agagctgggc cagctgctgg accacaacgg cgcgatcggc gacagcgagc gcaatgcggc
    23041 gcgcatcttc aatgccgacc actgcttctt cgtcaccaac ggcaccagca ccagcaacaa
    23101 gatcgtctgg caccacacgg tggcgcctgg ggacgtggtg gtggtggacc gcaactgcca
    23161 caagagcaac ctgcacgcca tcatcatgac cggcgcgatc ccggtgtttc tcaagcccac
    23221 gcgcaaccac tacggcatca tcggcccgat tccgcgcagc gagttcgagc ccgagaccat
    23281 caaggccaag atccgcgcca acccgctgct gcagcatgtc gacgccgatc gcgtcaagcc
    23341 gcgcatcctg acgctgacgc agtccaccta cgacggcgtg ctgtacgaca ccgaggcgat
    23401 caagggcatg ctcgacggct acgtcgacaa cctgcatttc gacgaggcct ggctgccgca
    23461 cgcggccttc catccgttct atggcagcta ccacgccatg ggcaagaagc gcgcgcggcc
    23521 gcagcagtcg gtggtgtatt cgacccagtc gatccacaag ctgctggccg gcatcagcca
    23581 ggccagccac gtgctggtgc aggactcgca gcaggtcaag ctcgaccgct acctgttcaa
    23641 cgaggcgtac ctgatgcaca cctcgaccag cccgcagtac agcatcatcg ccagctgcga
    23701 cgtggccgcc gccatgatgg agccgcccgg cggcaccgcc atggtggaag agagcattgc
    23761 cgaggcgctg gactttcgcc gcgccatgcg caaggtcgag gccgacttcg gcaagggcga
    23821 ctggtggttc aaggtctggg ggccggagaa gatcgccgaa gaaggcatcg ggcgcgccga
    23881 cgactgggtc atcaagaacg gcgcgaagga ccgcagctgg cacggctttg gcaagctggc
    23941 gccgggcttc aacatgctgg acccgatcaa ggccaccatc gtcacgcccg gcctgaacct
    24001 ggacggcaag ttcgacaaga gcggcattcc ggcgtcgatc gtcaccaaat tcctggccga
    24061 gcacggcgtg gtggtggaaa agaccgggct ctacagcttc ttcatcatgt tcaccatcgg
    24121 catcaccaag ggccgctgga acacgctggt gacggcgctg cagcagttca aggacgacta
    24181 cgaccgcaac cagccgatgt ggcgcatcat gccggagttc tgcgcccagc accggcgcta
    24241 cgagaagatg ggcctgcgcg acctgtgcca gcacgtgcat cagctctacg ccaagtacga
    24301 catcgcgcgc ctgaccaccg acatgtacct gagcgagctg acgccggcca tgaacccggc
    24361 cgacgcctac gcctgcatcg cccgccgcca ggtggagcgg gtggagatcg accagctcga
    24421 aggccgcatc accacggcgc tggtgacgcc ctatccgccc ggcattccgc tgctgattcc
    24481 gggcgaggtg ttcaaccgca agatcgtcga ctacctgaag ttcgcgcgcg acttcgcgct
    24541 cgagtgcccc ggcttcgaga ccgacatcca cggcctggtg gaggtggaag acgaccacgg
    24601 ccatgtgcgc tactttgccg attgcgtggc cgccgatgcc cgtggggtgc gcggcggttt
    24661 atgatgaagc gtgtgaccac tgaggagaac cgatgtttcc tgaataccgc gatctgatca
    24721 cccgtctcaa gggcgagggc tcgaacaccc gtttcctgaa cctgttcgag cggcacaacg
    24781 aactggatca ccagatcaag gccatggaag gccacgatgc cggtgccatc cacagcgaga
    24841 tcgagacgct gaagaaggaa aaactgcgcg tcaaggacga gctgtacacg atcctgcggc
    24901 aggaggccac ggtcggcaag tgagccgtcc atcgcgcgcc gcacaaaccc gcctcgccca
    24961 ggcgggtttt ttgtggactt ttatgtgctc tggcgcttat ctggcaagcg ccaaaagcta
    25021 tcggattggt agcgtttcag aagcgaatcc cggccgggtt ggtggcgttg cgcggggggg
    25081 cgatgctggg ctcggccggc gttgccgcgg cgggggcagt ggcttcgcgg gccgtaccgg
    25141 tggccgcggc gggcacgggg ctttgggcca ccagcggtgt ggcggccggg cgctgatagg
    25201 ccgccggcaa ctgcgaggtt ctgggatagc cggcggcgtc gaggtactgg ctccactcct
    25261 ggtccgaata gccgctcagg cgctgaccgc cgatggtcag caccggcacc tggctggcac
    25321 cggccagccg ctgcagggct gcgatgtcct cgttggactg cacggttttt tcactgaacg
    25381 gcacgccgcg ctggatcagc aggttgcggc cgctgacgca gggcgggcaa tcggcggagg
    25441 tgtagagcgt gacgggaaag cgctgcacgg tctggctgag cgtgtagggc agcgtggcgc
    25501 tgccgctgcg ccgggtatcg ccgccgagcg gggccacctg gcgacccgcc tgtgcgggag
    25561 gcggctggtc gctgtagctg acgccgccgg gcccgctcga acggtacacc tgcgcctgga
    25621 cgccgcccgc cagcagcagg gcgctcaagg ccgtgacgag aagcagaggg tgacgacgat
    25681 gcacgggcga ttctcctgcg ggcggggtgg acgacaaggg ctgcgggccg gccgccgtgc
    25741 gcctcaagcc atgccctggt ggcgcagcag cgcgtccagg ctgggctcgc ggccacggaa
    25801 ggccttgaac gattccatgg ccgggcggct gccgccggct tccagaatct cgcgtcggta
    25861 ttttcgtccg gtttcgacgc tgggcaagcc ctcgggcgtc acgctttcct cgaacgcggc
    25921 gtaggcgtcg gccgacagca cttcggccca cttgtagctg tagtagcccg cggcgtaacc
    25981 gccagcgaag atgtggctga aggtctgtgc cgtgcgcgcg aacggtggcg gctgcagcac
    26041 cgccacttcg cgtcgcaccg cgtccagcag cggctggaag tcctggccgc tggcgtgctc
    26101 ggtgtgcagc agcatgtcga acagggcgaa ctcgatctgt cgcacggtct gcaggccggc
    26161 ctggtagttg cgggcggcga tcatcctgtc gaacagctcg cgcggcagcg gctcgccgct
    26221 gtcgacatgg gccgtcatgt ggcgcagcac ttcccattcc cagcagaagt tctccatgaa
    26281 ctggctgggc agctcgaccg catcccactc gacgccgctg atgcccgaga cgtcgcgctc
    26341 gttcacctgc gtcagcagat ggtgcaggcc gtggccgaat tcgtggaaca gggtgatgac
    26401 gtcgtcgtgc gtgagcagcg gcggcgcgcc atgaacgccc tcggcgaagt tgcacaccag
    26461 ctgggccacg ggggtctgca ggcggccgtt gtccgggcgc agccagcggg cacgcacgtc
    26521 gtccatccag gcgccgccgc gcttgccggt gcgcgcgcca gggtcgagat agaactggcc
    26581 cagcagttgc ccgtcgcgct cgatgcggta gaactcgacc gaggggtgcc agaccggcgc
    26641 cgcgtcgcgg cggatggcga cttcgaacag ggtctcgacg atcttgaaca gcccggccag
    26701 caccttgggc gcctgaaagt actgcttgac ctcctgctcg ctgaaggcgt agcgcgcttc
    26761 cttgagccgt tcgcccagat agggccagtc ccagctctgc ggatcggcca ggtccagccg
    26821 ttcgcgcgcg aaggcgcgca ggtcggccac gtcgcgctcg gcgtagggcc gggccttggt
    26881 cgccagctgg cgcagaaagt cgatcacctg gcgcggcgat tcggccatct tgggcaccag
    26941 ggacacgtcg ccgtagctgg cgtagcccag cagctgcgct tcttcgtggc gcagcgccag
    27001 gatttcgcgg atcagcggtg cgttgtcaaa gcgcgtggca tcgccttcgg cctggtcgga
    27061 ggcgcgcgtg acgtaggcgc ggtacaggcg ctggcgcagc gcgcggtcat gcgcgaactg
    27121 catcgccggc aggtagctgg gcagcttcag ggtgagcttg tagccggact ggccgtcggc
    27181 ctgggcggcg gcgcgggccg tggccaggac atcgggcggc aggccttcga cctcggcttc
    27241 gctggcataa caggcgtagg cgtcggtggc gtcgagcgcg ttctcgctga acttctggct
    27301 cagctcggcc agacgctcct ggatggcggc gtagcgctgc ttggctgcac cctgcagctc
    27361 ggcgccgccc agcacgaagt cgcgcagggc gttgtcgcgc gcgcgccgct gcgcggcgtc
    27421 cagcggcgcc gggtcgatgg ccttgtagcg ggcgtacagc cgctcgtcgc tgcccagccg
    27481 ggtccagaac tcggtgaccc ggggcagggc ggcattgtag gcggcgcgca gctcgggggt
    27541 gtccatgacg gcgttgaggt ggctgaccac gctccaggcg cggcccaggc gctcgccggg
    27601 cacgtcgagc gtgcgcgaca gccacgtcca gtcggccggg gagtcggggc tgacggcggc
    27661 ctccagcgcc gcctcggctt cggccagcag ctggtcgatg gcgggagcca cgtgttccgg
    27721 gcggatctgg tcgaagcgcg gcaggtcgga gaagtcgagc aggggattgg tcatggcgct
    27781 tcagctgggg acaaggcggt gtttgtcaag ccgcaaagta tcaaaatgat agcttctggc
    27841 gctttgtgga caagcgccgc aggccatttt gcttaaagat cgagggtctg cgggcggccg
    27901 gcgccgcgcg gccccgggtc aggcggcggc gcgctcggcg gcctcgatgg tgttgaccag
    27961 cagcatggcg cgcgtcatgg gtcccacgcc gccgggcacc ggggtgatcc agctggccac
    28021 ctggcgcacc ccggtgaagt ccacgtcgcc gcacagcttg ccggcgtcgt cgcggttcat
    28081 gccgacgtcg atgaccaccg cgccgggctt gaccatgtcc gccgtcagga cgttgcgctt
    28141 gccgacggcg gccaccacga tgtcggcctg gcgcgtcagc gccgccaggt cggaggtgcc
    28201 gctgtggcag acggtgacgg tggcgttctg cgtcagcagc atcatggcca tcggcttgcc
    28261 gacgatgttg ctgcgcccga tcaccaccgc gtgccggccc cgcagatcgt gcaggccgat
    28321 cgactgcagc atgcgcatgc agccgtaggg ggtgcagggg cgaaagccgg gctggcccac
    28381 catcagcgcg ccggcgctgg cgacgtgaaa gccgtccacg tccttggctg gggcaatggc
    28441 ctcgatcacc gcatgcgcgt ccatgtgtgg cggcagcggc agctgcacca ggatgccgtg
    28501 cacggccggg tcggcgttga gggcggcgat gcgcgccagc aactcggcct gcggcagctc
    28561 ggccgggtag cgttccagcg tcgccgacag gccggtctgc tggctgtcgg ccaccttgct
    28621 gcgcacgtag acctggctgg ccgggttgtc accgaccaga atgatgctca ggtgcggttg
    28681 cacgccgcgt gcctgcagcg cctgcacgcg gtgcgccacc tcggcgcgga cggtggccgc
    28741 cagggcgttg ccgtcgatca gttgggcggt catgtgaaga acccgtcgga taccaatgaa
    28801 aaagctgcct ggcgcttgat ggatatgcgc cggcagctat gaaaacgata gtagctcaga
    28861 ccttggcggt ttgctgcccc agggcgatct tgagcaggtc ggccacggtg ttggcgccca
    28921 gcttttccat gatgttggcg cggtgcgcct cgacggtctt gatgctgatg cccaggtcgt
    28981 cggcgatctg cttgttcagg cggccggcga cgatgcgttc gagcacctgc gcttcgcggc
    29041 cggtgagctt ggccagcagc gcgtcgcggc tggcggcctg ctggtgttcg gcgaagctcg
    29101 tgcgggcctg ctccagcatg cgctcgacca ggggcatcag gtggtcttcg ttgaagggct
    29161 tctggatgaa gtccatggcc cccttcttca tggtgtcgac cgccagcggc acgtcgccat
    29221 ggccggtgat gaaggccatc ggcaggggcg agtggcgctc gttgaggcgg tcctggagct
    29281 ccagcccgct catgccgtcc atgcgaatgt cgatgatcag gcaggccact tcgcgcgggt
    29341 cgtagcgggc cagaaaggcc tcggccgact cgaagcagcg tacgcggtaa tcctggcctt
    29401 ccagcagcca ctgcaccgaa tcacgcaccg cctcgtcgtc gtccaccacg tagaccgtgc
    29461 ctcgtttcgg aatcagactc atgcaacttc cctgaggttg tggtcggacg ggcgggcatc
    29521 ctgtgccgga ccgaccggga tccagaaact gaagcggcag cccacgacct ggccggcatt
    29581 gtagaggttc tcggcgtgga tccggccctg gtgcgactcg acgatcgagc ggcacagatt
    29641 gaggccgatg cccaggccgt cgctcttggt ggagaagaag gcttcgtaca gccgcgccag
    29701 cgcctcgggc gccagaccgc gcccgttgtc ggtcacgctg aactgcaccg tgggctgatc
    29761 ttcgatgtcg cgctgcacca cgcgcagctc gatcaggcgc tggcccggcg cacgctgggc
    29821 gacgtcgatc gattcggcgg cgttcttgat caggttcatc agcacctgct cgatcaggat
    29881 cgggtccacg tgcagggccg gcaggtcggt ggcgatctgg cgcaccagcc gcacgttgcg
    29941 gcggcgcagt tcgatctcgg ccagctccag cacctgctcg accatcggcg tcacctcggc
    30001 ccaggcacgg cgcggctcgc tgcgcttgac gaagctgcgg atgtgctgca ccacctgacc
    30061 ggcgcgctgc gcctggcggg cggtcttgtc gagtgcgccg agcagatcgt cacggctgat
    30121 ctgcccgctg cgcacgcgcg agatcatgcc ggtgcagtag ttgctgattg ccgtcagcgg
    30181 ctggttcagc tcgtgggcga cgctggaagc catctcgccc atggtgatca ggcggctggc
    30241 ggcttcggcg cgctcggcct gcgcggcggc ctgctcctcg gcctgccggc gcgccgtgat
    30301 gtcggtggcg atcaccatct gcaccaggcg gccgtccacc cagttcaggt agcgcgagcg
    30361 cacttccagc cacttgttca ggcgttcgat gtagacctcg gcatgctctg ccagcggcgc
    30421 gttggccagg ctgtcgaccg gcagaccgac cagggagtcg accgtgtcca ggctctcttc
    30481 ggacgcctgg gtttcgggcg ttccggcgcg ctccaccagc ttcaggtggc cggcggtggt
    30541 gccgtcgaac cactggcgat acagcttgtt ggcaaacagc agctcctggc tgcccagcgg
    30601 cgccaccgac accgaagcgt ccagcgcttc cagcacggtg gtgaagcgct cgtacgaggc
    30661 cgacagctgg cgccgcaccc ggttcggttc ggtgatgtcg gtcatcgacg ccatccagcc
    30721 ggcatgcttg ccggtggcgt cgatcagggg cgagacgtac aggcgggcat cgaacagcga
    30781 gccgtccttg cgcttgacgc tcatctggaa gccggccggg tcggccgtgc cgcgcagctc
    30841 ctccttcagg cgcgacgaca gctcggcgcg gtcggcgtcg ggccaatagc tgaacggctc
    30901 ggcctggccc agcagctctt cctcgctcca gcccgtcatc tggcagaaag ccgggttgac
    30961 gtagatgatg cgcccctgca gatcgagcgc gcgcatgccg gtgagcatgg agttttccat
    31021 cgcccgacgg aagctggttt cggccagcag cgcatgctgc gcctgctgac ggcgttgcga
    31081 ctggcgccag ttggcgatca acatccatgc cgtcatgctg gccagcaccg ccaccagcca
    31141 gaacagcgtg ccaccgacca cgccttgtga ggtgcgccag gcctgggcgc gcagcaccag
    31201 cccgttgccg accggggaga ccgggctgtc gaaggcgttg acctgctcgc tccagggcag
    31261 gtactccgac aggccggtgc ggtccgggat ggtctggccg gccagcagct ggcccttggc
    31321 gtccagcagc gacacggcgt atttggccag cacctccgac ggcacgccat agcgcagcag
    31381 gccatcgacc gagtattccg ccagcaccac gccggcgaac tgcgcgcgcg gcgtgatcgg
    31441 cacgtgcagg tacagcaccg catcggcgtc ctcgacggcc agcggctggt tgtacacggc
    31501 ctggcgcaga tcctgtgcca ggctgtagcc ctcgctgacc tcgccgggct tgagccggcc
    31561 gtcggcgatc cagtcgggct ggggcgacac gctgggcgaa gtctgcgtgg tgcgcagccg
    31621 gcgctggccg tcgatccagc tgaccgcgcg caactccggg tactgcgcca ccatggcctc
    31681 ggcgcgggcg tcgaattgca gcgccgtcat ttcgcggtcg gccagctcgc gcgccagccg
    31741 catcagctgc tcctggcgct ccagcaggcg caggcgcagg cgttgctggc tgtattccac
    31801 gtcacgtttg acggcctcct gctcgcgccg cgtttcctcc aggcgcaagt accagaaggc
    31861 cgacacgatg gccgccagaa acagcagcac cgccgccagc ggggccagct gggcgatcgg
    31921 atcctgacct ttgagcaggc gctgccagcg tgtccggggc ggaccggagt cgtacagggt
    31981 ggaggcaagt tcggactgca tgcatgtcca gtgtacgtgg ctgaagacgg ccgtggcggc
    32041 gtgggtgcgc actccggccc gtcagggccg tcgaggcgct ggctggagat gcgctgcgga
    32101 caagggtgcg accgatcaga attgcgtatt atgagataac aaattactat ttgaaattgc
    32161 gccgatttgt gcgatcatcg ccgctggttg ggtcacaatg acttcgacaa catgagagga
    32221 gacgcgaaga tgtccaacaa tccacaggcg cagtctgctg ccgaactgga tgcccaggaa
    32281 acccgtgaat ggctggacgc gctgacggcg gtcatcgagc gcgagggccc cgagcgcgcc
    32341 cacgcgctgc tggaaggcct gctggagcac gcccgccagg ccagcatcga cctgccgttt
    32401 tccgccaaca ccggctacgt caacacgctc gagcctgagc aggaggcgcg ttgcccgggc
    32461 aacatcgcga tcgaaaagcg cctgcgcgcc tacatgcgct ggaacgccat ggccatggtg
    32521 gtgcgggcca accgcatcga cccgcccgaa ggcggcagcc tgggtggcca catcggctcg
    32581 ttcgcttcgc tggccagcat gtggggcgcc ggcttcaacc atttctggca tgccgagagc
    32641 gaacaccacg gtggcgactg catctacttt cagggtcaca gtgcgcccgg catctatgcc
    32701 cgcgcctacc tggaaggtcg catcaacgaa gagcagctgg aaaacttccg ccaggaggtc
    32761 aagggcaagg gcctgtccag ctacccgcac ccgcacctga tgcctggctt ctggcagtac
    32821 tccaccgtga gcatgggcct ggggccgatg atggcgatct accaggcgcg cttcatgaag
    32881 tacctgcacg cgcgcggcat cgccaacacc gaagggcgca aagtttgggt gtttctgggc
    32941 gacggcgaaa tggacgagcc cgaaagccgc ggcgccatca cgctggcttc gcgcgagaac
    33001 ctggacaacc tgatcttcgt catcaactgc aacctgcagc gcctcgatgg gccggtgcgc
    33061 ggcaacggca agatcatcca ggagctggaa ggcgacttcc gtggcgccgg ctggaacgtc
    33121 atcaagctgc tgtggggcaa gggctgggac gacctgctgc ggcgcgacaa ggacggcaag
    33181 ctgcgccagc tcatgatgga aacgctggat ggcgactacc agaccttccg cgccaaggat
    33241 ggcgcctaca tccgccagca tttcttcggc aggtaccccg agacggccaa gctggtcgaa
    33301 cacctgaccg acgatgaaat ctgggatctg cgccgtggtg gccacgatcc ggagaaggtg
    33361 tacgccgcct tccatgccgc caatgcgcac cagggcgagc ccacggtgct gctggtcaag
    33421 accgtcaagg gctacggcat gggcagggcc ggcgagggcc gcaacaccgt gcaccagacc
    33481 aagctgctgg acgacgagga catccgctac atccgcgacc gcttcaacat cccgattccc
    33541 gacagcgagc tcgacaagct gccctactac aagccggccg acgacacgcc ggagatgaaa
    33601 tacctgcacg agcgccgcca ggccctgggt ggctatctgc cgcaccggcg cgagaaggcc
    33661 gacgagagct tcaccatccc ctcgctggag accttcaagg cggtgatcga acccaccgcc
    33721 gagggccgcg agatatccac cacccaggcc tatgtgcgct tcctcacgca gctcttgcgc
    33781 gacaaggcgc tgggcccgcg cgtggtgccg atcctggtcg acgaggcgcg caccttcggc
    33841 atggaggggc tgttccgcca gatcggcatc tacaacccca agggtcagct ctacacgccg
    33901 caggatcgcg agcaggtgat gtactacaag gagtcggtcg acggccagat cctgcaggag
    33961 ggcatcaccg aagccggtgg catgtgttcg tggatcgcgg cggcgacctc gtactcgacc
    34021 aacaaccgca tcatgatccc gttctacatc tactactcga tgttcgggtt tcagcgtttc
    34081 ggtgacctgg cctgggcggc gggtgacatg ctggcgcgcg gcttcattct gggcggcacc
    34141 tccggacgca ccaccttgaa cggcgagggc ctgcagcacg aggatggcca cagccacatt
    34201 ctggctggca ccattccgca ctgcctgacc tacgacccga gcttcgcgca cgaggtggcg
    34261 gtgatcctgc acgacggcct gaagcgcatg gtcgagaagc aggaaaacgt cttctactac
    34321 atcaccctgc tgaacgagaa ctacgccatg ccgggcctca cgccgggcac cgagcagcag
    34381 atcctgcagg gcatgtacct gtgcaaaccc ggcgccgagg gcgagcggcg cgtgcagctg
    34441 ctgggctcgg gctcgatcct gcgcgaatcg ctggaggcgc agaagctgct ggccaccgac
    34501 tggggcgtgc aggccgatgt gtggagctgc ccgagcttca accagctcac gcgcgaaggc
    34561 caggacgtcg aacgctggaa cctgctgcac ccgctggagc agccgcgcgt gcccttcgtg
    34621 gcgcgccagc tggccggcca cccgggcccg gtggtggcgt cgaccgacta cctcaagaac
    34681 tacgccgagc agatccgccc cttcgtgccc aaggaccggc cctacatcgt gctgggcacc
    34741 gacggtttcg gtcgcagcga cttccgcgcc cgcctgcgcg agcacttcga ggtcgatcgc
    34801 cactacatcg cggtggcggc gctcaagggt ctggccgatg ccggcgcgct gcccaggcag
    34861 aaggtggccg atgccatccg caagtacggc atcgacaccg aaaagatcaa cccgctgcac
    34921 gcctgaggga gacgagtcat ggcactggtg gaagtcaagg tcccggacat cggcgacttt
    34981 tcggacgtgg cggtgatcga gctgctggtg gcgcccggcg acaccgtgaa gctcgaacaa
    35041 agcctgatca cggtggagtc ggacaaggcg tcgatggaga tcccctccag cgccgccggc
    35101 gtggtcaggg agctcaaggt caagatcggc gacaaggtca gccagggttc gctggtgctg
    35161 ttgctcgaat ccgcggattc ggcatcaaag ccggctccgg cgcaggtgca gcaagcgcca
    35221 gtagctattg atacaatagc aaacgcggcg cccccggcgg ctgcatcggt ggccagcggg
    35281 ccggtcgagg tgcgcgtgcc cgacatcggt gacttcaagg acgtggcggt gatcgaggtg
    35341 ttcgtccagc ccggcgacac gatcagggtc gaacaaagcc tgatcacggt ggagtccgac
    35401 aaggcctcga tggaaatccc ctccagcgcc gccggggtgg tgagggagat gaaggtcaag
    35461 gtcggcgaca aggtcaacca gggcgatctg ctggcgctgc tggaaggcgc cgcaccggtg
    35521 gccgcagcgc cggccgcggc ggcgccggcc aacgccccgg cgcccgccgc cgtgtcggcc
    35581 ccggcggcgc cgccgttggc ggccgcaccc gcgcacgaac ccacgcgcgc gccgctcggt
    35641 ctgccgcatg ccagccccag cgtgcgcaag ttcgcgcgcg agctcggggt gccgctggcc
    35701 gaggtcaagg gcagcggccc caagggccgc atcacgcagg aagacgtgca ggccttcacc
    35761 cgcgccgtga tgagcggcgc gcagcgcacg caggcgcagg gcgcggccgg cgtgtccggt
    35821 ggcggcgtca ccggcggcgg cgagctgaac ctgatcccct ggccgaaggt ggactttgcc
    35881 aagtttggcc ccatcgagcg caaggagctc agtcgcatca agaagatcag cggcgccaac
    35941 ctgatgcgca acgcggtcat gatcccggcc gtcaccaacc atgacgatgc cgacatcacc
    36001 gacctggagg ccttccgcgt ggccaccaac aaggaaaacg agaagagcgg cgtcaaggtc
    36061 accatgctgg ccttcctgat caaggcctgc gtggccgcgc tcaagaaatt ccccgagttc
    36121 aacagcagcc tggacggcga cgccctggtc tacaagaact actggcacat cggctttgcg
    36181 gccgacacgc ccaacggcct ggtcgtgccc gtcatcaagg acgccgacaa gaagggcgtg
    36241 ctgcagatca gcaccgagat gagcgagctc gccaagaagg cgcgcgacgg caagctgggc
    36301 ccggccgaca tgagcggcgc caccttcacc atcagcagcc tgggcggcat tggcgggcgc
    36361 tacttcacgc ccatcatcaa cgcgcccgag gtcgccatcc tgggtgtgtg ccgctccaac
    36421 atcgagccgg tgtggaatgg cgagaagttc aagccgcgcc tgatgctgcc gctgtcgctc
    36481 acctgggacc accgcgtgat cgacggcgcc gcggccgcgc gcttcaacgc ctatctgggc
    36541 cagatcctgg cggacttccg ccgcgtgctg ctgtgaagga gacgcggaca tggccgtgat
    36601 agacatcaag gtgccggaca tcggcgactt cgccgacgtc ggcgtgatcg agctgctggt
    36661 gaagcccggc gacaccgtca aggtcgagca gagcctgatc accgtcgaat ccgacaaggc
    36721 gtcgatggag attccgtcca gccacgccgg cgtggtcaag gaactcaagg tcaaactggg
    36781 cgacaaggtg agcgaaggct cggtgattct gacgctggag gccgcagggg cctcggccac
    36841 ctctcccgcc ggggcgggag agggcaggga tgagggtggg gttcaagcgg gagcgcaacc
    36901 ttcaccctca ccccaacccc ctcccgccag cggcagaggg cgtgaacccg cccccatcgc
    36961 cagcagcttt gccggcacgg ccgatctgga ctgcgacgtg ctggtgctgg gcggcggccc
    37021 cggcggctat tcggccgcct ttcgcgccgc cgacctgggc ctcaaggtgg tgctggtcga
    37081 gcgctacgcc accctgggcg gcgtgtgcct gaacgtgggc tgcatcccgt ccaaggcgct
    37141 gctgcacgtg gccgcggtga tggacgaagt gggccatctg gccgcgctgg gcgtggactt
    37201 tggcaagccc aagctcgaca tcgacaagct gcgcagccac aaggaaaagg tgatcgccaa
    37261 gctgacgggc ggcctgggcg ccatggccaa gatgcgcaaa gttacgctgg tgcgcggcta
    37321 cggcagcttc gtgggcgcca accacctgca ggtcgaagaa accaccggca gcggccagga
    37381 caagaccggc tctaagcgcg tggtgcagtt caagcgcgcc atcatcgccg ccggcagcca
    37441 ggccgtgcgc ctgcccttca tgcccgagga cccgcgcgtg gtggacagca ccggcgcgct
    37501 cgcgctcaag gccgtgccca agaagatgct gattctgggc ggcggcatca tcggcctgga
    37561 gatgggcacg gtgtacagca cgctgggcgc gcgcctggac gtggtggaaa tgctcgacgg
    37621 cctgatgcag ggcgccgacc gcgacctggt caaggtatgg cagaagatga acgcgccgcg
    37681 cttcgaccac atcatgctca agaccaagac cgtgggtgcc aaggccacgg acaagggcat
    37741 cgaggtggtg tttgaaggcg agggcgctcc cgcgccgcag acctacgatc tggtgctcca
    37801 ggccgtgggc cgcagcccca acggcaagaa gatcggcgcc gaccaggcgg gcgtggccgt
    37861 gaccgagcgc ggcttcatcg acgtcgatat ccagatgcgc accaacgtgc cgcacatctt
    37921 cgccatcggc gacatcgtcg gccagcccat gctggcgcac aaggcggtgc acgaggcgca
    37981 tgtggcggcc gaggtcatcg ccggtgaatt gaagggcgac gaagccctgg cgcgcgccgc
    38041 cttcaatgcc cgggtcatcc ccagcgtggc ctacaccgac cccgaggttg cctgggtcgg
    38101 cctgaccgaa gaccaggcca aggcgcaggg catcaaggtg aaaaagggcc tgttcccctg
    38161 gagcgcctcc ggccgggcca ttgccaacgg ccgcgacgag ggctacacca agctgctgtt
    38221 cgacgaagcc acgcaccgca tcgtcggtgg cggcatcgtc ggcacgcacg ccggcgacat
    38281 gatcggcgag gtggcgctgg ccatcgaaat gggcgccgac gcggtggaca tcggcaagac
    38341 catccacccg caccccacgc tgggcgagag catcggcatg gccgccgaag tcgcgcatgg
    38401 ttcatgcacc gacgtgccgc cgcaacgcaa atgaagctat tacatattta gctggccgcg
    38461 cagtgccaga aagggctgcg cggcttttct cccgcaagag cggcggctgg cgaaacacgc
    38521 cgtgccggcg acggcatggc gcccgcggaa cgtcgatccc atgtcaggcg gggcagatct
    38581 ccttccgtgc ctgagccgca tttgcggcgc tgagcgattc agtccgcgtg gcttggcggt
    38641 ggggctagaa cgtttgaccc acatcattca cgctctgggt tgcaggccag acgtcccaca
    38701 gccgcgtggc cgaactcgta tccttggtca acgggcagag actgtgtcgc ttcgccgcgg
    38761 aagtcgaaga tcacccgagc atgacttgac gccatggcgg gcgcgttcca ggttatgctg
    38821 gcgccaccct gttcgacgca agaggccgac atggcatggc acacttggct gatctatttt
    38881 gtagcggcgc tcggcctgtc gctgtcgcct gggcccaacg ggctgctggc gctgacgcac
    38941 ggtgccttgc atgggcgtcg taagacgctg tttaccgtcg ccggtggcgc gctgggtttt
    39001 gccaccgtca tcgccttgtc gatgttcggc atcggggccc tattgcaggc ctcgctggtc
    39061 tggctgaccg tgctcaagtg ggtgggtggc ggctatctgg tttggctggg cgtgcaggtg
    39121 tggcgcgccc cgcccattgc tgcgctggcc ctgcaaccgc agggtgaggt caacggctgg
    39181 tccttgtttc gacagggcgc gctgtcggcg ctgaccaatc ccaagggcat tctgttcttc
    39241 gctgcgttcc tgccacagtt catcgatccg gcacgcagcc tgctgctgca gtacttcatc
    39301 atggccggca ccttcgttgc catcgaattc atcaccgagg tgctgatagc aagcatggcg
    39361 cagcgcatca gcggctggct ggcgcgcgtg ggacgcggtt tcaaccgcgt gtgtggcggc
    39421 atctttgctg cgatcggggt ggcgctgccg ctgcgcgcct gagcgcggtc gtggcggcgc
    39481 tgtgcacccg tcaggtgtgc cgcagtggtg ctgccgggta cgtggcgtgc gcaaccacca
    39541 gctgaagatc aacgaagcaa ccggacccgg tgttccaccg tcgctcacca agatggcgag
    39601 cggtcaattg ccgttcctgg gggtcatgtg gatggtggcg tcatcgccag atggcggtga
    39661 cgctccaacc attgcatcaa cggcaaggcg agcgcgattt tctcctgggt gccgaggctg
    39721 ctgttccaga tttcgcgcat gcatgcggca tgcacctcga agtactgcgg ctgtgaaaaa
    39781 tcccagctgc gcgacaggct gcccggtttg tagaacttgt gacacagcag ctcgggcacc
    39841 cgcacgaagc tgcccagcag ggccagatgg aggacccagg gccagtcggc cgagaactcg
    39901 cccgcgccgt gcttcttcag tccgccgatc tggcgagcca tcgacagccg gaacagccca
    39961 cggttgggca cccaccactt gcctctgcgc tccagcacgc gcacgccgcg gatgaacgcc
    40021 gatcgctcgc catccagcgt ggggtagctc cagaactcct gacctccctg ggtgtcggtc
    40081 agcagcacgt ccgagaaggc cacgcaggcc tgaggatcgg catccagctc cgccgccagg
    40141 cgggcaatgt aggtgggcgc cagcaggtca tcgtggaagg cgaacagcgc atagtcgccc
    40201 tgcgcctgcg acagcaggaa gttgctgttg ccgacccagc ccaggcgcga ggtctgcctg
    40261 acgacgcgaa aacgggggtc gcgcagcgca tggcgctcgc agatcgcctg ggtgtcgtcg
    40321 tcgcaaaggt ccaccgacac cagcacgtgt agcgcaggat gggtctgcgc cgacagggcg
    40381 tcgagcgtgg gttgtatgaa ttcggcagcc tggtaggcgg gtaccagcgc ggttacggcc
    40441 gatggtgaga tcgtgctcat ggtgcagcgg ccccatcggg atcgacgtcg gtccaggtct
    40501 gtgccagcgc cgtgcgctgg cccagcaaca ggtcggccgc ctgcacggcg taccaggggg
    40561 cggtcgtgag cttgccgctg tccacgctca gatagcgggc atcgccgcgg gggccattga
    40621 aggagcgcgc atgaagaccg ctgtcgagcc gatccacatc ttcgcgccca taggcgaaga
    40681 tggcgcccgc gtccacgttc agcacggtgg cgcgatgcag gcccgggtac cagcgatcgg
    40741 ctgcgcgcag gatcgcctcg gccagtgcct gggcctgatc gggcgataca tcaccacggc
    40801 agggcccatc ccactcgggc ggcggtacca gggcttcact ccagccgcgc aggccttccg
    40861 ggtaccagga gacgaaggcc gtgccattgg ggcgcatcac cacgtcgcca tagcggccca
    40921 gcaccatcgt gaccgaactg aggtgctccc agcctgcagg cagccggacg atgacccggt
    40981 acttgagtcg gtgcagccag ccgggtgccg gctgcagtcc catgctggca tccagcccca
    41041 ggcgctgctc ccaggtggcg ttcaccacct gccgtgcaca ggcctccagc ggctccagcg
    41101 gttcgccgcc ggaccccagc acctggccgt ggagggtgac accgtccgga gccggtgcga
    41161 accgggtgat gcgccgccca ggcagccagt cgatgagtgg atgggtgatc accgcacgcc
    41221 gcagcgcagc ggccagcgcg tcggtgtcga tcgcgcgctc ggcggtggtg aacaccgatt
    41281 cgccgggagc gagtaccagg ccctggtgct cgtcccgaga tgccggccag gccagccgcg
    41341 tggggcgctg gcccaggtag ctggcctcgg ggtgctgggt catccaatgc ttgtacagcg
    41401 cctgcacgcg atcaaagtga cgcgccaggg tgggcgccgg caggaccgaa tcgcgagcca
    41461 cgaggtaggc gaacggggtg gacagcggca aggccgcggc caccggccga cccagccagc
    41521 gctccagcag gtgctgaaac accagtgcgc cgcgcaactg cacgcgggcc gtcgcgccgc
    41581 tggcgtcggc agcatagatc agacccagat gaatcttgcc ttcgttgcgc aggctggcgc
    41641 ggttcatggg cagcgcatcc tgatccagca gcaccacggg ctgacctctg tgcgcaagct
    41701 ccagcgcgat ggcgcagcct tgcaggccgg cgccaaccac ccacacgcgg gaatcgggaa
    41761 cgctgcggct catggctcgt gcaagagtga ccgctcatcg cccggcgttg gtcgtggtgc
    41821 gcgcctgccc tggcaattgc catgcgcctg catccacgtc gcgagcatcg ctggtaccgt
    41881 cataggcgtg ccaggcgatc aagtgcgttg tcgagcgtgc cgtcctgttt ggcgaagcaa
    41941 aaacgaatca ccttctggtc gaagccatca ccatagaacg ccgacagcgg aatcgctgcc
    42001 acgccgatct cgcgtgtcag ccactggcag aagtcggctt cggacaggtc acgctcgggc
    42061 accgagagtt catcgatcgc cgcgcactgg aaatacgtgc cttcgcaagg cagcagccga
    42121 aagcgcgtct gcgccaggcc ggcgcgaaac aggtcgcgct tggcggcgta gaaggcgggc
    42181 agttccaggt agggccgggg gtcctgcatg tacgcggcaa tgccgtactg catcggtgtg
    42241 ttgacagtga acacgttgaa ctggtgcacc ttgcggaact ccgccgtcag tacggccggc
    42301 gcagctacgt agccgacttt ccagccggtg acgtgacagg ttttgccaaa gctgctgacg
    42361 atgaagctgc gcgccgccag gccggggtag cgcgccgcgc tttcgtggcg cagtggggcg
    42421 tagaccatgt gctcgtacac ctcgtcgctg atcaccagcg cgtcggtggg cgccagcagc
    42481 tcctgcaggc gcagcatgtc ctgcgctgac cagaccatgc cgctggggtt gtgcgggctg
    42541 ttgatcagga tggcccgggt gcgcggcgac agagcggcgg cgaggcggtc gaagtccggc
    42601 cgaaagctgc cgggcgtcag cggcacgcgc accacgcggg cgccgctgag ttcgatgttc
    42661 ggcacgtagc tgtcgtagca gggctccagc acgatcactt cgtcgcccgg gccggcgcag
    42721 cacaggatgg cggtcaggat ggcctgggtg gcgccggcgg tgacggtgat ttcggcgccg
    42781 gcatcgtagt ggcggccgta cagcgcctcg atcttggctg cgatggcctc gcgcagcgcc
    42841 ggcacaccgg gcatcggcgg gtattggttc aaaccctgcc gcatggccgc cgtcacggcg
    42901 tcgaccagcg ccgggtcgca gtcgaagtcc ggaaaaccct ggcccaggtt gacggcctgg
    42961 tgctgcgtcg ccagcgccga catgacggtg aagatggtgg tgccgacgtt cggcaggcgc
    43021 gaagggacgg ccggcgtgcg ctcagagttc atagtcgttc aggtggccgc tcatggcctt
    43081 gagaatgagg tccttgtcca gccggtcgga cagcaggctg gcaaaatggt agacgaagtc
    43141 gcgcagccag gcgccgcgct tgagcgcgat gcgcgccacg ttctgtccca gcagcggacc
    43201 cagcggtcgc gccaccaggt cgccctggcg atcgtcggcc atggccatct cggcgacgat
    43261 gcctatgccc aggcccagcc gcacgtaggt cttgatgacg tccgaatcga tcgcctccag
    43321 caccaccctt ggcgtcagct ggcgctgggc gaacgcggca tcgacccggg tgcgcccggt
    43381 gaacgagggg tggtaggtga tcaggggctg tgccgcaatg tcctccagcg ccaggcgctc
    43441 tttttttgag agcggatggg tggccggcag gaccagcaca tgctgccact cgtagcaggg
    43501 cagggtgacc agttcggggt agctggccag cgactcggtg gctacgccga tctcggccgt
    43561 ctcctcgatc accatgcgcg ccacctgatc gggcgcgccc tggtgcaggc tgatgttgac
    43621 tttggggtat tgctcacgca ggcgcgccac cggcctgggc agcacgtaac gtgcctgagt
    43681 gtgggtggtg gcgatcgaca gcgtgccggt gtcctgcgcg ctgtactgct cgccgatgcg
    43741 cttgaggttg ccgacctcgc gcaggatgat ggcgatgctg tccagcacat gctggcccgg
    43801 ctcggtgatg cgcttgaggc gcttgccgtg gcgcgtgaag atgtcgatgc cgagctcttc
    43861 ttccagctcg atgatggcct tggacacgcc cggctgcgag gtgtgcagcg ccttggcggc
    43921 ctcggtcagg ttcaggttgc gtcgggcggc ctcctgaacg aaacggaact gatgaagatt
    43981 catggagatt ccgaatatag aactccatca ttatggctga atcgctctaa gacgagggcg
    44041 tatccagcat cgcatgggcc atggcggcca gcacctcggg gtgctccgag atcgccggct
    44101 gcaggctgaa acgcacctgc gggtgctgcg tgcgcagcac ctgcagcaac cgcggcaggt
    44161 cttcgcgcgc atggcggccg acgcccagga acatgggcca gacggtgacg gcgtcggcac
    44221 cgccggccag caaccggtcc acggcggtgg gcagatcggg cgcggtgtgc tccagatagg
    44281 cgcaggccac cgggcggccc ggcgcaagct ggcggatgcg ctcggccacg gcttcgatcg
    44341 gcgcgcgcca tgccgcttcg cgcgagccgt gcgcgaacag cacgatgccg tggcgatcgg
    44401 gcccttgcat ggctattgcc gcagcaccag ccagccgaag gcggccagcg acagcaccga
    44461 ataaatcacg ccaggcgccg ccgccgccag ccagggctcc cagttgcgca gattgccaat
    44521 gaagccgaac acgttgttga gcaggaagaa gctgataccc accagcacgc cgacgaacac
    44581 gtagccggtg atgttgccgg tgcgaaagtg caggtaggcg aacggcagcg ccagcaccac
    44641 catcaccagg cagctgagcg ggtagaacac cttgcgccag aactcgatct cgtagcgctg
    44701 cgcgctctgc tggttgctct gcaggtggcg gatgtagcgg aacaggtcca gcgtctgcat
    44761 gcggttgggg ttgagcaggg cggcggccac ggtgtcctgc gacagcgcgt tgggccagac
    44821 ccattcgtcc agacgctgca cttgcacccg cggttcggcc tgcggcacct gctcgaagtt
    44881 gcgctgctcc acagcctgca gcgtccaacc ctgatcgctg aaatggccac ggctggcctt
    44941 ggtcatggtc agcaagcgtc cgtcggcgat gctgaactga aagatctgca cttggttgag
    45001 cgtgccgtcg gccgacatgg tgcccacgtt gaccgcgtac tggctgtcgt cgcgccgctc
    45061 cttcagccag gcgccggtgc gccccaccgt gatgtcgccc agaaagcgcg cctgcaggtt
    45121 ctgcaccgcg cggttggcct ccggggccac gtagtcgccg cacacgaagg tcagcgccac
    45181 gaagcccagc gccagcacca gcagcatgcc cagcgcccgc aacggcccca gaccgctggt
    45241 gcgcaggatg gtgaactgcg agctggcggc cagctgcgcc atcacgtaga tggtgccgat
    45301 cagcaccgcg atcggcagca gctcgtacag gtggctgggc acctgcagcg ccacgttcag
    45361 cagcgcgtgg cctgcgtggt agccgcgtcc ggcgccgcgc tcaagctgac ccagggcgtc
    45421 gaccaggtcg aagaagaaga acagcgccag aaacgccagc gccacgaagc cgacggcacg
    45481 cagcacctcc cggtagatca gatggcggat ggtgttcacg cgccaccccc gttcagccgg
    45541 cggcgccagg cgctgcccca gccgtggtgg cgcgccagca gccagccaag cgacagcagc
    45601 agcacgccgc cgtgcagcag cagcagatag gccaccagcc cgacgcggcc ccgggcgatc
    45661 cagccttcgc cgaacgtcag caggttgtag taggtggtga aggcgaacag cgcgaacacc
    45721 agtccggcgc tgcgccccac gcgcgggttc acgcgcgtgg cggcgagcgc gaacaccgcg
    45781 cagttgagcg ccgccaggat cagcccgatg cgccacgaca actcaccctg atggcggggc
    45841 gtgggctggc tcagcagggt cagcgtcggc ttcatggtgg gcgaatcgtt ggcggtcggt
    45901 cccaccaggc gcgtgctgat gcggttgccg tattcggcga actcgctcac ccgcgtctgg
    45961 ccagtggcgc tgtcgagctc cagccggtgg ccgtggtcga gcaccacgaa gcgttccccc
    46021 ttgatcacct ccaggcgccc gcgctgcgcc gtggtcacgg tttccttgcc gtgttcgttg
    46081 gtggcgatga agatgttgtg gccggtctcg gcgtcggcac ggtccttgtc gatgaagaac
    46141 acgcgctgcc ccccggccga ctcgatgaag cgccccggcg ccacccgctc gatgtcgcca
    46201 cgcgcctggt agcggtcgcg cagctcgcga atctggtgat tggcccaggg ccacaccgcc
    46261 agcgccagca ccgccaccac cagcagcacc ggccaggcaa agcgcagcac gggcctgaga
    46321 aacgccgcca ggccctggcc gctggcgaac cacaccacca tctcgctggc cgcgtacatg
    46381 cgcgtcagcg tggcggtgat ggcgacgaac aggctcaggg tgaggatggt ggccaggtgc
    46441 ccgagcacgg tgtagcccat caccagcatc acttccgagg ggttgacgcg gccggccgag
    46501 gcctggccca gcgtgcggat cagcagcacc gtcatcacca ccgtgaccag caccaccagc
    46561 gtggcactga aggcccggcc gagctccttg cgaatggagg aatcgaataa catcagtcga
    46621 aaggaaaacg gcaattatgg acttcgagct tcaatccctt cgtctggccg acgcggccgc
    46681 ccgcaagtcg cccgccctga tggtgctggt ggccgacggc ttcgccggtg gcgaagatgc
    46741 gctgtcggcg ctgatcgcgc gcactgtggc cagcggcgac ttcaagaccg aggccggcgc
    46801 ccagctgttc gtctatggcg cggcaggcat cgtgcccgag cgcgtggtgc tggccggcat
    46861 cggcgacggc agtccggccc gggtgcgcaa ggcggtgggc acggccatgg gcgcgctgcg
    46921 cggtggcccg gccaaggccg tcacgctgtg ctttgcccag cccgccgaag ccgatgcgct
    46981 gcgcgccgcc gtgctggccc tggccgacgc cagctacacc tacagcgcca ccaagagcaa
    47041 accgaatgac aagaaggagc cgccgcgcat cggtaaggcc gtgctggggg tgccggaacc
    47101 gggcccgctg cgcgaggcgt tgcagcaggc ggtggcggtg gcgcgcggcg tcgaactggc
    47161 gcgcgaatgg gccaaccggc caggcaacca tgccacgccg accctgctgg cggacgcggc
    47221 ccggcaactg ggcaagctgc cacgcatcgg ctgcgaggtg ctgggtccga aggaggtggc
    47281 gcgcctgggc atgggcgcct tccaggcggt ggcgcagggg tcgcggcagg agttgcgctt
    47341 catcgtgctg cgctacgacg gcgcgccccg gtcgcaggcg ccggtggtgc tggtgggcaa
    47401 gggcatcacc ttcgacaccg gcggcatctc gatcaagccg gcgcccgaga tggacgagat
    47461 gaaattcgac atgagtggcg ccgccagcgt gctgggcgtg ttccgcgcgc tggccgagct
    47521 gaggccggcg ctcaacgtgg tgggcctgat cccggcctgc gagaacatgc ccgacggtgg
    47581 cgccatcaag ccgggcgatg tggtcaccag catgagcggt cagaccatcg aaatcctgaa
    47641 caccgacgcc gaaggacggc tggtgctgtg cgacgcgctg acctatgccg agcgcttcaa
    47701 gccatgtgcg gtgatcgaca tcgccaccct gaccggggcc tgcgtgatcg ccctgggcgg
    47761 cgtgcgcagc ggcatgtttg ccagcgacga tgcgctggcc caggccctgc agagcgctgg
    47821 cgagcaggcg ctcgaccctt gctggcgcat gccgctggac gacgaatatg gcgaggcgct
    47881 caagagcaac tttgccgatg tgggcaacgt gggcgggcgc ccggccggcg cagtgacggc
    47941 ggccaagttc ctgcagcgct ttgccgacaa gttcagctgg gctcacctgg acatcgccgg
    48001 taccgcctgg aagggcggca gcgccaaggg cgccaccggc cgcccggtgg ggctgctggt
    48061 gcattacctg ctgatgcagg cgggacggcc ggtcaaggcc ggtggcgagc cgaccaagcg
    48121 tgccaaaacc aagggtcagg ccggcgccag ggccggaaaa ggcggcaagg cctgacacat
    48181 gctgccgacc cggtggccgc gatgaccgca gtggcgtttc acttcaacgt gccggacaag
    48241 ctgggctacg cctgccgctt gctgcgcaag gcgtacgcag ccggtggccc ggtgggggtg
    48301 gtggcgccga gcgggatgct gagcgagctc gacacccggt tgtgggcctt ttccgcgctc
    48361 gatttcgtgc cccattgcct ggccgacgcg ccggcatcga tgcgggcggt gacgccgatc
    48421 gtgctggccg aagattgcgc ccagctgccg ccggcgaccg cggtgctggt caacctgggc
    48481 cctcaggtgc cggacggctt cgagcgtttc gggcgtgtga tcgagctggt cacgggtgac
    48541 gatgacgatc gcgcgcaggc gcgccagcgc tggcgccact acgccgggcg cggactggag
    48601 attcaacgcc atgatctggc cgccggagcg taagccatga acccggacga cgaaccgtcg
    48661 gcgcgaccgc cgccgcgtgg ggtgccgacc ctgaccgagg tggtgcaggc cgcggcgccg
    48721 ccgtctgaag cgcctgcgcc cgcgagcctg ccgcagccgg actcgccggt ctcgccgacc
    48781 tcgcatgtgg ccctggagcc ggccgtgccg gcgcaggacc cggtggatgc gctgctggag
    48841 cggctggggc ccgaactgga gcgccagatc gccgaggcgg tcggccgtgt gctgcacgaa
    48901 cagatgctgg gtttcaacag ccgagtgcgc aaggcggtgg ccgatgtggt gcgcgacacg
    48961 gtggcggcca gccttctgca ggccggcggg cgttcggatg tcagtgaaaa ccctgagaat
    49021 cagccttccg acacctagcg tgttccctag tggccggtag cccaaaaacc tcgtatcgtt
    49081 cgcgccccag aagcccagtc gtgggcttca tttcatcgga ggtatttccc atcatgcagg
    49141 ttcaactgaa actgaccgtg gccgctgctc tggccctcgt tgccggcctg gccagcgccc
    49201 aggaacaggt cgtcaagatc gccagcgtgg cgcccatgtc gggcggccag gcgcactatg
    49261 gcaaggacaa cggcaacggc gtgacgatgg cgatcgaaga cctgaacaag cagaacatca
    49321 ccatcggcgg caagaagatc aagtgggaag ccgtgatcga agacgacgcc gccgatccca
    49381 agcagggcgc tgcggtggcg cagaagctgt gcgacatgaa ggtcaacggc gtggtcggtc
    49441 acctgaactc gggcaccacc atcccggcgt ccaagatcta tcacgagtgc ggc
//
LOCUS       DAOQTZ010000009        46253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_891_length_46253_cov_13.814472, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000009 DAOQTZ010000000
VERSION     DAOQTZ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 46253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46253
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_46253_cov_13.814472"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKN51_02205"
     CDS             complement(<1..284)
                     /locus_tag="PKN51_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNT83942.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKLGEVH
                     DGAATTDWMEQEQERGITITSAAVTCFWKGMDLSYPEHRFNIIDTPGHVD"
     gene            complement(350..820)
                     /gene="rpsG"
                     /locus_tag="PKN51_02210"
     CDS             complement(350..820)
                     /gene="rpsG"
                     /locus_tag="PKN51_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNT83943.1"
                     /translation="MPRRREVPKREILPDPKYGNVELSKFMNVIMEGGKKAVAERIIY
                     GALETMEKKSGKDPLELFITAINNVKPMVEVKSRRVGGANYQVPVEVRPVRRLALSMR
                     WIKEAARKRGEKSMALRLANELLEANEGRGGAMKRRDEVHRMAEANKAFSHFRF"
     gene            complement(993..1370)
                     /gene="rpsL"
                     /locus_tag="PKN51_02215"
     CDS             complement(993..1370)
                     /gene="rpsL"
                     /locus_tag="PKN51_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNT83944.1"
                     /translation="MPTISQLVRHGRTVETTKSKSPAMQNSPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGSL
                     DLQGVKDRKQSRSKYGAKRPKKA"
     gene            complement(1706..2866)
                     /locus_tag="PKN51_02220"
     CDS             complement(1706..2866)
                     /locus_tag="PKN51_02220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNT83945.1"
                     /translation="MIPLLSRTLVATLLAVTTLVGHAQAPQAPEVAAKAYLLMDVSAG
                     QVLAAKDIDAPVEPASLTKLMTAYLVFDALKARKISLNQTLPVSERAWKMPGSRMFID
                     PKMQVPVEDLIKGMIVQSGNDATMALAEGVGGSAERFVQLMNEQAKAMGLAGTQYRNP
                     EGLTEPGHHTTARDLATLSTRLLRDFPEYLHYYTIRKYRYPGTPASNDTNRNTLLFRD
                     PTVDGLKTGHTSAAGYCLVATARRDFPNLPGRRLLSVVLGASSETARANESQKLLNWG
                     YTAYEAVKLFDGGQPVASPKVWKGKSAELKLGRGEPIVVAVPSGSAGQIKTEIARPEP
                     LIAPFRQGQAVATLKVRQGEQTLAEVPLVALEAVEEAGFLGRAWDAMRLWIK"
     gene            complement(2937..3572)
                     /locus_tag="PKN51_02225"
     CDS             complement(2937..3572)
                     /locus_tag="PKN51_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT83946.1"
                     /translation="MNAQTESLTLRGPAGAIEALLDRPAAGGAARGTAVIAHPHPQFG
                     GTMSNKVVQTLARACVQCGWRAVRFNFRGVGASAGQYDHGQGEQDDLLAVVAQQAAAD
                     QALLLSGFSFGAFVASQAIARLHPQRALAGAVLVGTAASRFDVAPLPPALHAQTLVVH
                     GEHDETVPLTSVMDWARPQLLPVTVVPGGGHFFHGQLPLLKGLVERHLRAL"
     gene            complement(3658..4002)
                     /locus_tag="PKN51_02230"
     CDS             complement(3658..4002)
                     /locus_tag="PKN51_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNT83947.1"
                     /translation="MSQSPPSSYYQRHIFFCLNQRDNGEACCADHDAQAAFDRCKARV
                     KAEGLAGPGKVRVNKAGCLDRCAAGPVAVVYPEGVWYSYVDVSDIDEIVDAHLKRGQI
                     VERLLTPPHLGR"
     gene            complement(4087..5187)
                     /locus_tag="PKN51_02235"
     CDS             complement(4087..5187)
                     /locus_tag="PKN51_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNT83948.1"
                     /translation="MRDSHPSAALPLALLYAALIVYASLYPFTGWRDVGVAPWAYLGA
                     PLPRYWTGFDVGANLAGYLPLGLLLTLALLHRRRVGSAVLLAALAAAVLSLLLEALQT
                     YLPQRVSSNVDWALNATGGLAGALLAGALERLGALDRGRRLRARWFLPDSRGALVLLA
                     LWPVGLLFPAPVAFGMGQLFERLEEGAARLLEDTPFLRWLPLRELDLQPLLPSEEALC
                     VALGALVPCLLGYTVIARAWRRALFALAALAAGVAVSALSAGLSYGPIHAWAWIGAPV
                     QQGLWLALLAAALLLPLPVRLCVPLLMVVLAAQVVLLNTAPQNPFYALTLQAWEQGRF
                     IHFHGLAQWIGWLWPYAAFVYLIGRVGTTGAR"
     gene            complement(5203..5634)
                     /locus_tag="PKN51_02240"
     CDS             complement(5203..5634)
                     /locus_tag="PKN51_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNT83949.1"
                     /translation="MLWIKALHIVFMASWFAGLFYLPRIFVNLAMVPPGSLAERQRLL
                     LMARKLYRFMTPLGAIALALGLWLWLGYGIGLTGPGNGWMHAKLLIVLLLIAYHAWCR
                     QLLARFEQDQERHGHVWFRWFNEAPVLMLLATVILVVVKPF"
     gene            5900..6910
                     /gene="hemB"
                     /locus_tag="PKN51_02245"
     CDS             5900..6910
                     /gene="hemB"
                     /locus_tag="PKN51_02245"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNT83950.1"
                     /translation="MKHLPLAPFPALRPRRLRRDDFTRRLVREHRLSVDDLIYPVFVL
                     DGKNRREAVPSMPGVERLSLDQLLPLAEECVQLGIPVLALFPVIDARLKDDKGSEAAN
                     AKGLIPRVVRELKMRFPGLGIMTDVALDPYTSHGQDGWLDKTGYILNDETVGQLVKQA
                     LTQAAAGVDIVAPSDMMDGRIGAIRDALEKKGHIHTRIMAYSAKYASAFYGPFRDAVG
                     SAKNLGASDKKVYQMDPGNSDEALREVAIDIAEGADMVMVKPGLPYLDIVRRVKDQFG
                     VPTFAYQVSGEYAMLKAAAQNGWLDHDRVMMESLLAFKRAGADGVLTYFARDAARLLQ
                     NQ"
     gene            6973..8097
                     /locus_tag="PKN51_02250"
     CDS             6973..8097
                     /locus_tag="PKN51_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNT83951.1"
                     /translation="MHIVEFTGGSLRFADAVPAAAPADGFVWIYLDREDFARHLPELQ
                     EAAHRLGGSPLLDVHAKDLANPAHPSGYDATSVYDLVVFRRLASLAEVQLNGNHDPHT
                     GAAGAPRAPKPQPFERIDSRAVGFVVFDRLLISVHPPGCQTAVGFLQRQADDARLSVD
                     SASARNRVPQGPDDLVLRMLNAMVDSYLDLRRDLSEALEQAQVELLKPHPAPHTWGAL
                     MHARGRLHGLQDLCEEQQDAMQEWLDTLRELPLSTYGPDPLLAQQRHDQLVARARDVM
                     EHIERVLRHARRLEQAAETAVQIHFAAQGQRTNDTMRALTAITAVFLPLNLFTGFFGM
                     NFEHLPLIHSVEGMWLALLLLVVLALGIVVLFWRRRYLTR"
     gene            8121..9056
                     /locus_tag="PKN51_02255"
     CDS             8121..9056
                     /locus_tag="PKN51_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT83952.1"
                     /translation="MDSLDLIQTFREVARRGSFSGAARALGMSPANVSKYIAQLEARF
                     GLRLFHRTTRKVTLTEAGELLFERSGALLELIDFTEGEMHERATRPVGRLALTVPHAL
                     MASELPALLGRFMQRYPEVTLHLHVTNRLVNLVDEGVDLAFRIGPILQPNLIVRRLLR
                     LDHVAAATPDYWDRRGRPAHPSELLEHDQLAFALPGEAPVWQFQDRGKPLELRLTPRF
                     TATDPAPLRTWALQGLGVVWGTVHWLADALASGTLEPLLHDYSPPEIWLYVAYMQRRH
                     SSAALRALLDYLDAQFQPPPTPTLPAPGRRRVDSR"
     gene            complement(9076..9765)
                     /locus_tag="PKN51_02260"
     CDS             complement(9076..9765)
                     /locus_tag="PKN51_02260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNT83953.1"
                     /translation="MGQTIELKAADGQLVPAYLAPPAGPARGAVVVLQEIFGVNRHIR
                     AVADGFAGDGYLALAPALFQRLRARVELGYEEADVKAGLALKAAAEALPAPGVLADVQ
                     AAIDHAAQASGGKVGVIGYCWGGLLSWRAAALLDRLAAAVPYYGGGMTQPAELARQPR
                     VPVLAHLSDHDASVPLDGVAALRRAHPEVELHLYPAAHGFNCDQRAAWQPAAAALARE
                     RTLAFFARHLR"
     gene            complement(9896..10483)
                     /locus_tag="PKN51_02265"
     CDS             complement(9896..10483)
                     /locus_tag="PKN51_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNT83954.1"
                     /translation="MAALALAGCALPLPDKPPRTEPYDLGPPPAAGAAATPGAPLALA
                     PIEASPALDSQAMVYRLLYAGAGQQPRPYAQARWSMPPPQLLALRLRQRLAATRPVLD
                     AGSALAALQLRVELDDFAHEFSSPQTSEGVVRLRVTALAAGARAQPLLGQRSFSVRRP
                     APTPDAAGGAQALRDATDEALRQLIDWLAGLPASG"
     gene            complement(10558..11547)
                     /locus_tag="PKN51_02270"
     CDS             complement(10558..11547)
                     /locus_tag="PKN51_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNT83955.1"
                     /translation="MENKAHALAAGTFVVLVTALLIGLAMWLLRDVTNTRAYEIVSHN
                     AVSGLQPQAAVRFKGVAVGKVTRISFDPAERGRVLVTLAIAPDAPITRATVAELAYQG
                     VTGLSFVQLDDDGSDAAPLPPGPNGVPRIPLKPGLLGQLGDRAQDLIGKLDTSAQRIN
                     QLLAPDNQAVLTGALGDIGAAARSVDRLARTADQTLQAQLATSRTSIPRLITQATDTL
                     RTLQDATTEARTVIHTLGQAAQQTQQGVARVTAPGGVIDRLDQGVQTINASTLPRLHG
                     LADAADQTLRRVDRVARTLGDNPQALVYGSGPVPPGPGEAGFVPPPAHPTSPR"
     gene            complement(11552..12406)
                     /locus_tag="PKN51_02275"
     CDS             complement(11552..12406)
                     /locus_tag="PKN51_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT83956.1"
                     /translation="MEAAVQASDAVDTALAPGQPVIEVRGLWSVFALPGGGQHVVHQD
                     LDLTVRRGEVLTLVGGSGTGKTVLLRHMLGLTRPARGQATVLGRPAGELAREGAAARV
                     GMLFQQGALFSAFSVLDNIAFALRESGALPPALVREAALLKLQMVGLKASDAHKMPAE
                     LSGGMIKRVALARALIMDPPLLMLDEPTAGLDPDSSDAFVALLRALHQELGLTVVMVT
                     HDLDTLFELSSRVAVLADKRVIINAPVPEVLAFEHPFIRHFFLGERGRRAMELLRQRQ
                     HARPAQGD"
     gene            complement(12573..13694)
                     /locus_tag="PKN51_02280"
     CDS             complement(12573..13694)
                     /locus_tag="PKN51_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT83957.1"
                     /translation="MSAEPPRIDHAPADAAARVLLRGEWTAAWLSTRPVWRATQAALG
                     GVAAGGSWDLRQLAALDHLGALVLWHHWGRRWPAELLSSDEQRALLERVAAGAAPAPP
                     AEARAGWSEGVEALGLRVTDAAFQVRDFVTLMGQLLLDALRLLARPGRGPWRDFSGHL
                     YRIGAQALPITALVGFLIGVVLAYLMSQVLRQFGAEAFIVNILGLALIRELGPILAAV
                     LLAGRSGSAITAQIGVMRVTEELDAMRVLGIPAGFRLVLPRALALAIAMPLISVWTTL
                     AALVGGMLASDITLGITPAYFVEALPKAVRIPNLTLAVSKSVVFGVSIALIGCHYGLR
                     VKPNTESLGQGTTASVVASITAVILIDALFAVLFKSVGI"
     gene            13879..14463
                     /locus_tag="PKN51_02285"
     CDS             13879..14463
                     /locus_tag="PKN51_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNT83958.1"
                     /translation="MALQGPFFGKRDEPTSPRGPGSTPLSGTSALGSAGAAGAQPFAA
                     PSAAASPAAPASTPVAASVTESGAKLIVGPNIKLKGVEITDCDTLVVEGMVEATMDSR
                     VIQIAEQGSFKGSAEIDIAEVRGSFDGNLTVRQKLVIFATGKVTGKIRYGKIVIEEGG
                     QLSGEISFGTDKDHKSDKPAASAGSSKGEQLAVA"
     gene            14537..15244
                     /locus_tag="PKN51_02290"
     CDS             14537..15244
                     /locus_tag="PKN51_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83959.1"
                     /translation="MLAAALALPLGGCAGSAPTTRGVVRGQDFQPAELLQSEGNRVAH
                     NAMQANQRSLLLLADKLYRRNPAEWRKRASTREAALADIERSLLTGQPWPPLGDRRDV
                     AALSYALAPDFMGDRVAAFVIACADTIVTAHGGKREFYYLDGVDPQHLYNAARNMEVA
                     LWILNSRRDAQGQPLLLANDIGPDRRNLSFEREFGKIIGRLDLLASYATERYRRAVIG
                     YVQGVVAAPLLAFLPVR"
     gene            15422..17116
                     /locus_tag="PKN51_02295"
     CDS             15422..17116
                     /locus_tag="PKN51_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNT83960.1"
                     /translation="MDELTFDHIIVGAGTAGSLLANRLSQDAGKRVLLIEAGGKDDYH
                     WIHIPVGYLYCIGNPRTDWLYQTEPADGLNGRTLRYPRGKVLGGCSSINGMLYLRGQA
                     RDYDHWAALTGEDEWRWEHCLPDFMAHEDHYRLDSDSRSSGAVGGANDQDWSGFHGHG
                     GEWRVERQRLRWQVLDAFAEAAVQAGLPASEDFNRGNNEGVGYFHVNQKNGWRWNTAK
                     AFLRPTCYGRSNFELWTRGQVMRLALERDADGALRCAGAEVWTGGEMQKARLAPGGEL
                     LLAAGSIGSPQILQLSGIGPAALLQQHGIAVVADLPGVGANLQDHLQIRSVYRVQGAQ
                     TLNQLASTLWGKARIGLQYLWSRSGPMSMAPSQLGAFTRSSPAYAWPNLEYHVQPLSL
                     DAFGEPLHRFPAITASVCNLNPTSRGTVRIRSHRFEDAPAIAPNYLSTDEDRQVAADS
                     LRVTRRIMAQPAMARFAPEEYKPGVQYQSDEELARLAGDIASTIFHPVGTTKMGRDDD
                     PMAVLDPRLRVRDGAGGLIRALRVVDAGAMPTITSGNTNSPTLMMAEKAARWIRGA"
     gene            complement(17299..17973)
                     /locus_tag="PKN51_02300"
     CDS             complement(17299..17973)
                     /locus_tag="PKN51_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNT83961.1"
                     /translation="MRSVQGWQGLRGWAARRARAAGRPSVPPRAHSALEDVQAILTGC
                     LFVALALVFMRDSRLLPGGVTGLAFIVHYASGWALGAALFVLNLPFYLFAWRALGWRF
                     TLKTFVAVAVLALYTELLPRLVGLARLDPVFSAVMSGLLAGTGILILMRHQASLGGLG
                     ALAFYLQQRRGWRAGTVQMLCDALILGSALWLLPPGRVALSVLSAAALNFVIAINHRP
                     DRYHGI"
     gene            18369..19061
                     /locus_tag="PKN51_02305"
     CDS             18369..19061
                     /locus_tag="PKN51_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNT83962.1"
                     /translation="MEFFSAPWWSALLAIVLIDLVLAGDNAIVIALAARNLPRERQRQ
                     AIVWGTVGAIGVRTLMTLVVVWLLKIPGLMALGGLGLLWIAYKLVADTGADESEHGPA
                     ASTFWGAMKTIVIADALMGIDNVLGVAGAAHGAMDLVVIGLLISVPIVVFGSTVVLRL
                     VERFPVIIQLGAAVLAWTAASMIAGEPLLAGWFGPREAPAQALRWALAALCIVGVLGA
                     AWLMRTRLQNDP"
     gene            19126..19470
                     /locus_tag="PKN51_02310"
     CDS             19126..19470
                     /locus_tag="PKN51_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNT83963.1"
                     /translation="MARMILKWLLSAAALLGVAYIYGGVSVSSFSTALLAALVIGLLN
                     MLVRPVLVLLTIPITLLTLGLFLFVINALMFWAASGLVGGFNVSGFWAALLGSLIYSA
                     LGLVIDALLERA"
     gene            complement(19648..21066)
                     /locus_tag="PKN51_02315"
     CDS             complement(19648..21066)
                     /locus_tag="PKN51_02315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNT83964.1"
                     /translation="MTALEERKLGDRIIRELYRDPDYIDDPIVGEYVDGLWRALLDGA
                     RARGELPPELDERYAWRVLLGRDRSINAFALPGGYFGLHLGLVGVVGSRAELASVLAH
                     ELSHITQRHIPRLLEQEKRQTPLMLAAMVAGMIAASRNPQAGAALAVGGQAAVIQQQL
                     NFSRDMEREADRIGYGVLVQAGFDGQGFVGMFEKLQNATRINDNGDWPYLRSHPLTTQ
                     RIADMQQRAPAVARGAAPELEALLVAARARVLGRPGVDVLRAWAGEPDQPGFAARPLA
                     ARAGALYAAALARAQLRELPRAQALASQLAQAVAGDAAAARQARLLQAELALAADQPA
                     RALELLPPERPSGRGQAGRATLLLRAQAQTASGQAAQAADALQVWVSDQPQDAGAWQA
                     LAQAHAAQGQTLRALRAEGEVPMAQLDYAGAVDRWRAAQDAARRAAGAADLIEASIVD
                     TRLRQAQALLQQQRQEEQKARR"
     gene            21312..21824
                     /gene="moaC"
                     /locus_tag="PKN51_02320"
     CDS             21312..21824
                     /gene="moaC"
                     /locus_tag="PKN51_02320"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNT83965.1"
                     /translation="MVSRAPASPLTHFDAQGQAHMVDVGAKPATHRVAVAGGRIEMQP
                     ATLALIESGNAKKGDVLGIARLAGIMAAKKTSELIPLCHPLALSRVAIEFEASGANGT
                     GPAAVFCTATAETTGPTGVEMEALTAVQVALLTIYDMCKAADRGMTITGVRLLEKRGG
                     KSGHFVAARA"
     gene            21836..23374
                     /locus_tag="PKN51_02325"
     CDS             21836..23374
                     /locus_tag="PKN51_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HNT83966.1"
                     /translation="MPPSLRHVARVLLLALPWLWPFTSGPTAATQPYLAGAALAALLL
                     LAWPRQRQAGWHALLDGWLLAALVSSAIALLQYFNLEAPLFPWVNQAQTGQAFGNLRQ
                     PNQLATLLVIGLLALAWQAQAGRLSPRAAAALGVPLLTALAASASRVGALELLLLGAL
                     CLWWARGSGQLRRAGALLAAAWLLYALAALALPWALQLSEGQQGRDLIERLRAGEGTC
                     GSRLILWRNVLHLIALRPWTGWGWGELDWAHYITLYPGERFCHILDHAHNLPLHLAVE
                     LGLPLTLLAAGVLAWAVWRARPWAERQPARQLAWAVLAAIGLHSLVEYPLWYAPFQIA
                     AACCLWLLWATRRGAPAEAGPAPGRRLLAVLLLAGVGCAGWDYHRISQIYLPREQRAA
                     AYRDDAMAHARRSWLFADAVRFAEVTTRPPTRDNAAWLLPAALQTLHYSPEPRVITQV
                     IESASLLGRDELALTHLARFRAAFPREYRQWSADNRQLLEDAQAQQRAAGSAPQAPAS
                     AMNK"
     gene            complement(23884..25446)
                     /locus_tag="PKN51_02330"
     CDS             complement(23884..25446)
                     /locus_tag="PKN51_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNT83967.1"
                     /translation="MGLSLIQSRTLMGLQAPAVTVEVHLASGLPSFTLVGLAEVEVKE
                     ARERVRSALLNSGLQFPHNQRITVNLAPADLPKDSGRFDLPIALGILAASGQIDGGRL
                     AGWEFAGELSLSGELRPVRGALALSVARQQAGVDTRLVLPPGSAGEAALVPGQVVYRA
                     RHLLDVVARFQPPHSQPPMQDSAFGPEDEVDAGWLRVPESPRPAAPAYPDLADVKGQA
                     AARRALEIAAAGGHSLLMVGPPGTGKSMLAQRFAGLLPPMDVDEALQSAAIASLDGSF
                     RLAHWGRRPTRAPHHSASAVALVGGGSPPRPGEISLAHQGVLFLDELPEFPRAALEAL
                     REPLESGHITISRAAQRAEFPARFQLIAAMNPCPCGYLGSRLKACRCTPDQVARYQGR
                     LSGPLLDRIDLHVEVGALAPEELLNAPAGESTAAVRARCVAARERALARQGQPNQALQ
                     GQAIDTHARLDAAARTFLHSAAARLGWSARATHRALRVARSIADLAGSEAVSLAHVAE
                     AVQYRRALSGLG"
     gene            25648..26412
                     /locus_tag="PKN51_02335"
     CDS             25648..26412
                     /locus_tag="PKN51_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HNT83968.1"
                     /translation="MHPTLSPLTPRRLALGLLCASAAGAWAQSSGAAAAATPEHSLSF
                     NVGLTSDYRYRGISQSRLKPAVSAGADYSHASGLYAGAWLTSIKWIKDAGGDAAAELD
                     LYAGYKGSAGPLAYDVGVLRYQYPRAHLAVSPNTTEIYGALSYGVFTAKYSHALTNLF
                     GFADSKGSGYLDLSANLDLGDGWSVVPHVGRQWVRHNSAFSYTDYAVTLNKDFGHGLV
                     ASAAVVGSNADKALYVTPSGRFTGRTALVLGLKYTF"
     gene            26473..26811
                     /gene="glnK"
                     /locus_tag="PKN51_02340"
     CDS             26473..26811
                     /gene="glnK"
                     /locus_tag="PKN51_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNT83969.1"
                     /translation="MKLVTAIIKPFKLDEVREALSALGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVIDFLPKVKLETAVADDQVERVIEAIEAAARTGKIGDGKIFVAALEQVVRI
                     RTGETGTDAL"
     gene            26832..28220
                     /gene="amt"
                     /locus_tag="PKN51_02345"
     CDS             26832..28220
                     /gene="amt"
                     /locus_tag="PKN51_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNT83970.1"
                     /translation="MKHPIAPLTLAFGLALALPGAAWAQAASDAAAAAPALAASAAAA
                     ASAAAAAAPAPKIDTGDTAWMLTSTLLVILMTIPGLALFYGGLGRAKNMLSVLVQVFV
                     IFALVSVLWALYGYSLAFSGEGNFFGGLDKLFLKGVTPETFGALTTIPEYVFLAFQGT
                     FAAITVALIVGAFAERARFSAVLLFAVLWFTFSYVPMAHIVWGGGLLAKDGALDFAGG
                     TVVHINAGIAGLVGAYLVGKRIGFGREALAPHSLTLTMVGASLLWVGWFGFNAGSAGA
                     ANAIAGLAFVNTVLATAAATLSWIALEAMHKGKASMLGAASGAVAGLVAVTPAAGFVG
                     PMGAIVMGVIAGACGLWGVSGLKRLLRVDDAFDVFGVHGVCGIVGAILTGVFAAQGLG
                     GTGGLTPDSFSMGAQVWIQIKSVLFTIVWSAVVSLIAFKIADLVLGLRVSEEDERQGL
                     DISSHGETAYSR"
     gene            28321..29391
                     /gene="glcE"
                     /locus_tag="PKN51_02350"
     CDS             28321..29391
                     /gene="glcE"
                     /locus_tag="PKN51_02350"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HNT83971.1"
                     /translation="MEAALQSLAAKITQAAARGRPLRIRGGGTKDFYGQQLHGELLDA
                     RPLAGVLAYEPSELVVTVGAGTRLRELEALLAEAGQHLPFEPPRFGVAGTVGGAVAAG
                     LSGPARASVGAARDFVLGAQLINGRGERLNFGGQVMKNVAGYDISRLLAGSLGTLGLI
                     TQLSLKVLPRAPAEATLVFALSETSARQQLNRWCGQPLPLSASAWADGQLWVRLRGAQ
                     AALASACRQMGGERLDEPRAQRQWDALRDQTLPFFRLAPDEALWRVSVPDTASPLNLG
                     PTLVEWHGAQRWLRLPPAEAPRVREVAARLGGHATLFRGGDGVTPVFTPLTPPLARIH
                     QALKREFDPAGIFCPGRLTPDF"
     gene            29391..30632
                     /gene="glcF"
                     /locus_tag="PKN51_02355"
     CDS             29391..30632
                     /gene="glcF"
                     /locus_tag="PKN51_02355"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HNT83972.1"
                     /translation="MQTALAPEYQGTALGQEAEAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGHAPTRDTQLHLDRCLTCLNCESTCPSGVQYGHLIDIGRQVV
                     EAKVARPPAEARTRALLRRGLSSPLFGPAMRLGQSLRGLLPATLRAKVPAPRPAGAWP
                     RRTHARKVLLLEGCVQPSMAPGINAATARVLDAAGIECVLARGQGCCGAIKYHLADGA
                     GGLAQMRANIDAWWPHIERGAVEAIVINASGCGAMVKDYGHLLRHEPPYAERAARVSA
                     MARDVSELLPDLVAALRGRVRPPAEVLAWHAPCTLQHGQRLRGGVEQHLGALGFRLRP
                     AAQEAHLCCGSAGTYSVLQPELSLRLRERKLGQLLDEGAADQPAAILSANIGCITHLQ
                     AGTEVPVRHWIELLDQALHAT"
     gene            30727..32325
                     /locus_tag="PKN51_02360"
     CDS             30727..32325
                     /locus_tag="PKN51_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HNT83973.1"
                     /translation="MTGIQDWTLPYASRRSPVLGRQVVSTSQPLAAQAGLRMLLAGGN
                     AVDAALAAAMALTVVEPSGCGIGSDAFAIVWDGQALHGLNASGRAPRAWTPDYFARLG
                     GIPDKGWNAVTVPGAVSGWIALWRRFGRLPLAQLAQPAIDYARHGFPVSPTIATLWTL
                     GAERLGAQPGFAECFLPAGRPPRAGELFRSEAHARTLEDIVATEGESFYRGALAARMA
                     AHARACGGAMTEDDLGAHQADWVGTLAQRFGDSVVHEIPPNGQGIAALMALGMLDELG
                     IGDAPVDSADSLHLQIEAMKLALADTYHYNADLAHMAVAPAQLLERGYLRQRAALIDR
                     QRAGEPTWGTPRPGGTVYLAAADAQGMMVSFIQSNYMGFGSGVVVPATGISLQNRGHG
                     FSSAAGHANQVGPGKRPSHTIIPAFAMHADGRPQMAFGVMGGPMQHQGHVQMALRVLR
                     HGQNPQAAADAPRWRVTGGRGVALEPAVDAGVVQALRARGHDVRVESGHGVFAFGGAQ
                     LVLRQDGHYVAGSDPRKDGGAVAF"
     gene            32515..33525
                     /locus_tag="PKN51_02365"
     CDS             32515..33525
                     /locus_tag="PKN51_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83974.1"
                     /translation="MRHPYTLIGLTPSERALLESLFALERDTSGAMVPTPDPREAHLI
                     VVNGDDHTLVEQLRQDNPRALIVLAGHPAGAPVTGLPVLRRPFDMPGVIEVLSGLDWP
                     AGLPCVAAGEFDRTFVSSAPTSSQLDTGAPPAVDPQAFAPTTASAPLAPAPQGRAVAV
                     PDRAPVSSRAAWARSELAPPPWSAAAAAAPGGFVDDREAEVLVVTGALGERSLTLPRG
                     IRRLGLRVRLLDGPAAVLAALDQGAASFVFLDQLSLGEQLLPLARALAARRAGNGAPP
                     HVVVVARRGTPLDRLRARLAGCHWMQVPIDGPRLVAFFARRGLRPRDGVGPAFSSTPD
                     DQ"
     gene            complement(33611..34513)
                     /locus_tag="PKN51_02370"
     CDS             complement(33611..34513)
                     /locus_tag="PKN51_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT83975.1"
                     /translation="MAAAHPPSALLPALALLGNALVWGLSWIAFKALHQHGLHPLWST
                     AVVFGVALLALLAWQPASLRAFARHPQLLVLAAAAGVTNIGFNWAVTLGDVVRVTLLF
                     YLMPVWSVGLAWWLLGERPTRAALLRLVLALAGLVLVLHRPGAPWPWPRGAADQLALL
                     GGAGFALTNVLLRRWRATPPPARVLAMFGGGFATATGLALAGLAGGLSPLALSTHGLA
                     WLPWVLGLTLAFLAGNLALQYGAARLPARVTALIMLAEVVFASASAVLLGAATPTRWT
                     WAGGVLILLAALLSILPMPRGRAG"
     gene            complement(34719..35999)
                     /gene="lplT"
                     /locus_tag="PKN51_02375"
     CDS             complement(34719..35999)
                     /gene="lplT"
                     /locus_tag="PKN51_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HNT83976.1"
                     /translation="MRRGFYTIMAAQFFSSLADNALFVAAVELLRGSGAAEWQRAALV
                     PMFALFYVVLAPWVGAFADSRPKGRVMFLSNAIKIVGCLAMLFGGHPLLAYAIVGLGA
                     AAYSPAKYGILTELLPPSQLVRANGWIEGLTIASIILGVLLGGQLVGPQLAPRLLGLD
                     LPGLHSGIDTPAEAAIASLVLVYAIAAAINLRIPHTGVRMRPLPARTLSLLPDFWRCN
                     ARLWRDKLGQISLATTTLFWGVSGNMRYIVLAWAAAALHYSTTQAAGLVGVVAIGTAA
                     GAVLASLRVRLDRAIAVMPLGILMGALVVLMVFIDRLWLAVPLLILMGALGGYIVVPM
                     NALLQHRGHNLMGAGRSIAVQNFNEQACILGLGALYSASTGAGLSAFGAIALFGGVVM
                     AFMLLIQRWHRHNLRRYPGKVERLLNIARRDRGH"
     gene            36129..37208
                     /gene="alr"
                     /locus_tag="PKN51_02380"
     CDS             36129..37208
                     /gene="alr"
                     /locus_tag="PKN51_02380"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNT83977.1"
                     /translation="MPRPIQATIHTGALRHNLERLRQAAPGAQLWAVVKANAYGHGIE
                     RVFEGLRGADGFALLDLAEAERVRALGWRGPILLLEGVFEPRDLELCSRLSLWHAVHD
                     DAQIDWLAMHKTHQPHHVFLKLNSGMNRLGFAPERLRAAHARLSALPQVGEISLMTHF
                     SDADGARGIAAQWAVFQRVTEGLPGERSVCNSAATLRHAQDAAVRADWVRPGIAAYGG
                     SPDFPAHDAAHWGLQPAMTLRSRLIATGSLQAGDSIGYGSTFTAERPMRVGVVACGYA
                     DGYPRHAPTGTPVLVDGVRTRLLGRVSMDMLSVDLEPVPQAGPGSAVTLWGQGLPIDE
                     VAHAAGTIGYELMCALAPRVPVQVD"
     gene            complement(37351..38109)
                     /locus_tag="PKN51_02385"
     CDS             complement(37351..38109)
                     /locus_tag="PKN51_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNT83978.1"
                     /translation="MDLLLVSLASLLAGGIDAIVGGGGLVLVPALFAVYPNAAPATLF
                     GTNKSASVWGTGIATFQYARRVQMRWAVLGPAALAALAGSFLGAWAVTLIDPTFLRRA
                     LPLILLAVLLYTLAKKDLGRTHAPRHERGRETLLACAIGAVIGGYDGFFGPGTGSFFI
                     FLFVRLLGYDFLHASAAAKLLNVATNVAAIALFAAKGHVWWQVGLLMAAANVTGSLIG
                     TRLALRHGAGFVRGVFIVVVGALILKTGYDAFLR"
     gene            complement(38463..39815)
                     /gene="argG"
                     /locus_tag="PKN51_02390"
     CDS             complement(38463..39815)
                     /gene="argG"
                     /locus_tag="PKN51_02390"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNT83979.1"
                     /translation="MSHTILQSLPAGQKVGIAFSGGLDTSAALLWMRQKGAVPYAYTA
                     NLGQPDESDYDAIPRKALEYGAEKARLVDCRTQLANEGIAALQCGAFHIQTAGATYYN
                     TTPLGRAVTGTMLVNAMKEDDVNIWGDGSTYKGNDIERFYRYGLLANPALKIYKPWLD
                     QRFIDELGGRKEMSEFMQKAGFDYKMSAEKAYSTDSNMLGATHEAKDLEELSSGIRIV
                     NPIMGVAFWKPEVEVKAEEVRVRFEEGRPVALNGREIADPVELFLEANRIGGRHGLGM
                     CDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQAIMLRETAQRWVAGAVTGEVTLELRRGNDYSILNTESPNLTYAPERLSMEK
                     VENAPFTPLDRIGQLTMRNLDITDTRGKLAVYSRSGLLQLGEGSMLPQLGHDSSASRK
                     "
     gene            complement(40006..40317)
                     /locus_tag="PKN51_02395"
     CDS             complement(40006..40317)
                     /locus_tag="PKN51_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNT83980.1"
                     /translation="MPSRPTAIPAELVELLDDAALSLHELARACAMSPEWVHAHVQAG
                     VLQPLGGGASTEWRFASLSLRRARRIAQLEQVYDADPQLAALTADLMEEVIALRQRLD
                     G"
     gene            complement(40457..41440)
                     /locus_tag="PKN51_02400"
     CDS             complement(40457..41440)
                     /locus_tag="PKN51_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNT83981.1"
                     /translation="MQFKDYYQILGVSHSASPEDIKKAYRKLARRYHPDVSKEPDAAR
                     RMAEVNEAHTVLSDPEKRAAYDSVGAQAWAAGARSGDDVRPPPGWNTGYEFSDGAAPG
                     FGPHGEGGEFSEFFEQLFGRARAGARGARPGAGAQAPLRGQDHHARIELELIDAYRGA
                     ERAIHLRGAHLDAQGQVRPQERTLQVRIPQGVKPGQLIRLAGQGEPGWHGGPAGDLFL
                     EVQFRPDARWRIDGRDVTQRLRVTPWEAALGGAVQVATPDGKTVEVGVPAGSGSGRKL
                     RLKGRGIPSASAPGDLLLELELAVPGAVTDAQKAAWARLAEAYPGFDPRGA"
     gene            complement(41613..42521)
                     /locus_tag="PKN51_02405"
     CDS             complement(41613..42521)
                     /locus_tag="PKN51_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT83982.1"
                     /translation="MSARREHLDGRAIAILLVCCLFWGVQQVMVKATLPELAPVFQAA
                     LRFVGATALLWLWCRWRGVPLFQRDGTLGAGLLAGALFAGEFAAMYLGLQYTTASRLT
                     VFLYTSPFWVALVLPWLVPAERLHRLQWLGLACAFAAVLFALREGFAQGGAGPTWRGD
                     LLALAAGALWGLTTVSIRATRLVRVAPEKMLFYQVAVSAVVLPLLSLALGEPWRWRWS
                     AFAASSLALQTVVGAFASYLAWMWLLAHYPATRMSAFSFLTPIFALMAGALWLGEPIT
                     VSLVVALALVALGMLLVNRRAPARSD"
     gene            complement(42669..43178)
                     /locus_tag="PKN51_02410"
     CDS             complement(42669..43178)
                     /locus_tag="PKN51_02410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNT83983.1"
                     /translation="MPHALRAHQPAFSAQEFRAALGMFATGVTIVSARSADGALVGLT
                     ANSFNSVSLDPPLVAWSLGLRSLSLHAFRRGSHYAINVLAAEQQELALRFAAPVGDRW
                     AGVAWHPGVAGAPLIEGAVAHFECFNRSRHDEGDHVLFIGQVEHCAHRPGAAPLLFHG
                     GRFYTEHPL"
     gene            43286..44080
                     /locus_tag="PKN51_02415"
     CDS             43286..44080
                     /locus_tag="PKN51_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNT83984.1"
                     /translation="MALLPSFATLGHGPPVVLLHGSGGGARSFAPQLESLASLGFRAI
                     AWDMPGYGQSAPIEPYGFKGLAERGAALIEALAPLTGGAPVALVGHGLGGMVAQELVL
                     RRPDLVRQLVLVASVAAVSADDGYGRHVAQALAWLDEGRDMGWIAAERVPRLIGPGAL
                     ASGVRLATHCQATVPAPTWRRALLALAGFDRRAALAHIAVPTLLVAGELDPLAPPPVL
                     RAMAAQITGAHSERLAHTGHLPQLERPQAFDRLLLPFLRHARAWRH"
     gene            complement(44154..44576)
                     /locus_tag="PKN51_02420"
     CDS             complement(44154..44576)
                     /locus_tag="PKN51_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT83985.1"
                     /translation="MKILLAVDGSAFTKKMLAYLTTHLDLFSGDIEYTALTVHGALPP
                     RARAAVGREIVETYYAEEAAKVLDPVVKFLTRHQLNVKTLSKVGNAGELIAKTAESGK
                     FDLIVMGSRGHGALGGLVMGSVATKVLASCEVPVLLVR"
     gene            complement(44803..44931)
                     /locus_tag="PKN51_02425"
                     /pseudo
     CDS             complement(44803..44931)
                     /locus_tag="PKN51_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            45096..45284
                     /locus_tag="PKN51_02430"
     CDS             45096..45284
                     /locus_tag="PKN51_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83986.1"
                     /translation="MTMWNVWIRHLTTLFAPLTRAPRHGPGVPASLLERADACAGRDP
                     RGAADLRAAALNYLGVVR"
     gene            45319..45597
                     /locus_tag="PKN51_02435"
     CDS             45319..45597
                     /locus_tag="PKN51_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT83987.1"
                     /translation="MSTTSLTLGRTAVREPQFEPARLDHWLPRPQTGGPSMSQLLRAR
                     WNQLRQRLAQARSEREIQALADLDHRVQRDLQTARDLAEWKVPHRPFV"
     gene            complement(45745..>46253)
                     /locus_tag="PKN51_02440"
     CDS             complement(45745..>46253)
                     /locus_tag="PKN51_02440"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HNT83988.1"
                     /translation="TMGSETTAAAFGAQGVVRRDPFAMLPFCGYNMADYFGHWLKLGQ
                     RLQAAGARLPAIYCVNWFRKDAAGKFVWPGYGENMRVLKWMIDRVEGRAAGQPTMFGV
                     APRHDEINWSGLSFSPAQFDTVTRIDADVWAQEFALHDELFTKLAQGLPPELLATRQA
                     LAQRLEAA"
BASE COUNT         6657 a        16691 c        16477 g         6428 t
ORIGIN      
        1 aagtccacgt ggccgggggt gtcgatgatg ttgaagcggt gctcggggta ggacaggtcc
       61 atacccttcc agaagcaggt cacggcggca gacgtgatgg tgatgccgcg ctcttgctcc
      121 tgctccatcc agtcggtggt ggcggcgccg tcgtgcactt cgcccagctt gtggttcacg
      181 ccggtgtaga acaggatgcg ctcggtcgtg gtggttttgc cggcgtcgat gtgcgccgag
      241 atgccgatgt tgcggtaacg ctcgatgggg gtcttgcgag ccatgatggg ttctttcttg
      301 acacgggcag gcccgctcgt ggcgggccgc cgctcgggaa gggagagatt cagaagcgga
      361 agtggctgaa ggccttgttg gcctcggcca tgcggtgcac ctcgtctcgg cgcttcatgg
      421 cgccgccgcg gccttcgttg gcctccagca gctcgttggc caggcgcagg gccatggact
      481 tttcgccgcg cttgcgcgcc gcttccttga tccagcgcat cgacagggcc aggcggcgca
      541 ccgggcgcac ctcgaccggc acctgatagt tggcgccacc gacgcggcgg ctcttgactt
      601 ccaccatcgg cttgacgttg ttgatggcag tgatgaacag ctcgagcggg tccttgccgc
      661 tcttcttttc catggtttcg agcgcgccgt agatgatgcg ctcggccacg gccttcttgc
      721 cgccttccat gatcacgttc atgaacttgg aaagctcgac gttgccgtac ttgggatccg
      781 gcaggatttc acgtttggga acttcgcgac gacgtggcat gactgacctt tcaatttgct
      841 tcagttggcg gccggagcag ggtttgacgt tctccgctcg cctggcaggc ccatcgggtg
      901 cctgcactta ctcgacccga accgcgggtc acttcgcctg atccggggtc gtcggacacc
      961 cgcaccagac accgaaaaaa cgcaacggcc gatcaggcct tcttgggacg cttggcgccg
     1021 tacttggagc gcgactgctt gcggtctttc acgccttgca ggtcgagcga gccgcgcacg
     1081 atgtggtagc gcacaccggg caggtccttg acccggccgc cgcgcaccag caccacgctg
     1141 tgctcttgca ggttgtggcc ttcgccaccg atgtaggaga tgacctcgaa gccgttggtc
     1201 aggcgcacct tggcgacctt gcgcagcgcc gagttcggct tcttgggcgt ggtggtgtac
     1261 acgcgcgtgc acacgccgcg gcgttgcggc gagttctgca tcgccgggct cttggacttg
     1321 gtcgtctcga ccgtccgccc gtgacgcacc agttgactga tggttggcat gaaattccct
     1381 tgaaacgtga aaacgcgcgt gcagcgcgcg ccgaaaaacc ccgcaacgcc agcaaggcga
     1441 gcgccctcac ccaagatggc gcgcgcggca aaaatacggg aaaagccgca gattatagcc
     1501 cgccggcgcg ggcgctacaa gccgctgccg cctgcgcgcc ggactcggag tgcgggcacg
     1561 ggttgcgggc gccagagagt cgcgccccaa cacgggcgat caaggcacca ggcacagccg
     1621 cagcctgagc ggcgcgagca tgggccgcgc cgcgcgggtg cacctgggcg atcgaggcgg
     1681 gcaggcggga tcgggtcacc ccgtctcact tgatccacag tcgcatggcg tcccaggccc
     1741 gacccaggaa gccggcctcc tcgaccgctt ccagcgccac cagcggcact tcggccaggg
     1801 tctgctcgcc ctggcgcacc ttcagcgtcg ccaccgcctg gccctggcgg aacggggcga
     1861 tcagcggctc ggggcgggcg atttcggtct tgatctggcc ggcgctgccg ctgggcacgg
     1921 cgaccacgat gggctcgccg cgacccagct tcagctcggc cgatttgccc ttccacacct
     1981 tggggctggc caccggctgg ccaccgtcga acagcttgac cgcctcgtag gcggtgtaac
     2041 cccagttgag cagcttctgc gactcgttgg cgcgcgccgt ctcgctgctg gcgcccagca
     2101 ccaccgacag caggcgccgg cccggcaggt tgggaaagtc gcgccgcgcc gtggccacca
     2161 ggcagtagcc ggcggcgctg gtgtggccgg tcttcaggcc atcgacggtg gggtcgcgaa
     2221 acagcagggt gttgcggttg gtgtcgttgc tggcgggcgt gccggggtag cggtacttgc
     2281 gaatggtgta gtagtgcagg tactccggaa agtcgcgcag cagccgggtg gacagggtgg
     2341 ccagatcgcg cgcggtggtg tggtggccgg gctcggtcag gccttcgggg ttgcggtact
     2401 gggtgccggc caggcccatg gccttggcct gctcgttcat cagctgcacg aagcgctcgg
     2461 cgctgccgcc caccccctcg gccagcgcca tggtggcgtc gttgccggac tgcacgatca
     2521 tgcccttgat caggtcttcc accggcacct gcatcttggg gtcgatgaac atgcgcgagc
     2581 cgggcatctt ccaggcgcgc tcggacaccg gcagggtctg gttcaggctg atttttctgg
     2641 ccttcagggc gtcgaacacc aggtaggccg tcatcagctt ggtgagcgag gccggctcca
     2701 cgggggcgtc gatgtccttg gcggccagca cctggccggc cgaaacgtcc atcagcaaat
     2761 aggccttggc ggccacttcg ggcgcctgcg gcgcctgggc gtgacccacc agggtggtga
     2821 ccgccagcag cgtggcaacc agggtacggg acagcaaagg gatcatcatg aactatgaaa
     2881 atgatagctt atggcgcttg ccagtcgggc gccagagcct gaaaaatctt ggaacatcaa
     2941 agggcgcgca ggtggcgttc gaccaggccc ttgagcagcg gcaattgtcc gtgaaagaag
     3001 tgaccacccc ccggcaccac ggtgaccggc agcagttgcg gccgcgccca gtccatcacc
     3061 gaggtcagcg gcacggtctc gtcgtgctcg ccatggacca ccagggtctg ggcgtgcagc
     3121 gccggcggca gcggcgccac gtcgaagcgc gaggccgccg tgcccaccag caccgcaccg
     3181 gccagcgccc gctgcgggtg cagtcgggcg atggcttgcg aggccacgaa ggcgccgaac
     3241 gaaaaaccgg acagcagcag ggcctggtcg gccgccgcct gctgcgccac cacggccagc
     3301 aggtcgtcct gctcgccctg gccgtggtcg tactgcccgg cactggcacc aacgccgcgg
     3361 aagttgaagc gcaccgcacg ccagccgcat tgcacgcagg cgcgcgccag ggtctgcacc
     3421 accttgttgc tcatggtgcc gccaaactgc gggtgcgggt gggcgatcac ggcggtgccg
     3481 cgcgctgcgc cgcccgccgc cggacgatcg agcagggctt cgatcgcacc ggctggcccg
     3541 cgcagcgtca gggattcggt ctgggcattc attcagctat ctaaattgta gctgctcgcg
     3601 cttgctgcgc cggcgccgga aggtgaaaaa gcttgtaacc cgacgcccgg tcgggcgtca
     3661 gcgtccgagg tgtggcggcg tcagcaggcg ctcgacgatc tggccgcgct tgaggtgcgc
     3721 gtcgacgatt tcgtcgatgt cgctgacgtc cacgtagctg taccagacgc cctccgggta
     3781 caccaccgcc accgggccgg cggcgcagcg gtccaggcag ccggccttgt tgacgcgcac
     3841 cttgcccggc ccggccagcc cctcggcctt gacgcgcgcc ttgcagcggt cgaaggcggc
     3901 ctgcgcgtcg tggtcggcgc agcaggcttc gccgttgtcg cgctggttga ggcagaagaa
     3961 gatgtggcgc tggtagtagg aagacggtgg cgactggctc atgcggcgat tctaggaggc
     4021 cgctgtggcc accggccgca gcggcgaccg gagccccgcg cgccggtgcg ggcttgacgc
     4081 gcgccgtcag cgtgcgccgg tggtgccgac gcgcccgatc aggtagacga aggctgcgta
     4141 cggccacagc cagccgatcc actgcgccag gccgtgaaag tggatgaagc gcccctgctc
     4201 ccaggcctgc agggtgaggg catagaacgg gttctgcggc gccgtgttga gcagcaccac
     4261 ctgcgccgcc agcaccacca tcagcagcgg cacgcacagc cgcaccggca gcggcaacag
     4321 cagggcggcc gccagcaacg ccagccacag cccctgctgc accggtgcgc cgatccaggc
     4381 ccaggcgtgg atcggcccgt aggacaggcc ggccgacagc gccgacaccg ccacccccgc
     4441 cgccagcgcc gccagcgcga acagcgcgcg ccgccaggcc cgcgcgatca ccgtgtagcc
     4501 cagcaggcag ggcaccagcg cgcccagggc cacgcacagc gcctcctcgc tcggcagcag
     4561 cggctgcagg tccagctcgc gcagcggcag ccagcgcaga aacggcgtgt cctccagcag
     4621 ccgcgccgcg ccctcttcca gccgctcgaa cagctgcccc atgccgaacg ccaccggcgc
     4681 cggaaacagc agccccaccg gccacagcgc cagcagcacc agcgcaccgc gcgaatcggg
     4741 caggaaccag cgcgcgcgca ggcggcgccc gcggtccagg gcacccagcc gctccagcgc
     4801 gccggccagc aacgcgccgg ccaggccacc ggtggcgttc agcgcccagt cgacgttgga
     4861 cgacacgcgc tgcggcaggt aggtctgcag cgcctccagc agcagcgaca gcactgccgc
     4921 cgccagcgcc gccagcagca ccgccgaccc gacgcggcgc cggtgcagca gcgccagcgt
     4981 cagcagcagc cccagcggca ggtagccggc caggttggcg ccgacgtcaa agccggtcca
     5041 gtagcgcggc agcggcgcgc ccagataggc ccagggcgcc acaccgacgt cgcgccagcc
     5101 ggtgaacggg tacagcgagg cgtagacgat cagcgccgcg tacaacagcg ccagcggcag
     5161 cgcggcggac gggtgggagt cgcgcaccgt cgcggcccgc cgtcagaacg gcttgaccac
     5221 caccaggatg acggtggcca gcagcatcag caccggcgct tcgttgaacc agcggaacca
     5281 gacatggccg tggcgctcct gatcctgctc gaagcgcgcc agcagctgcc ggcaccaggc
     5341 gtggtaggcg atcagcagca gcacgatcag cagcttggcg tgcatccagc cgttgcccgg
     5401 cccggtcagg ccgatgccgt agcccagcca cagccacagc cccagcgcca gcgcgatggc
     5461 gcccagcggc gtcatgaagc ggtacagctt gcgcgccatc agcagcagcc gctgacgttc
     5521 ggccagcgaa ccgggcggca ccatggccag gttgacgaag atgcgcggca ggtagaacag
     5581 gccggcaaac cagctggcca tgaaaacgat gtgcagggcc ttgatccaga gcatgcggcg
     5641 agtgtagcca gccaggggcg gcgcaccggt gccgcgcgcc cataaaaaac cccggccaga
     5701 ggccggggtt gaaaagcctg tctgctgtca cacatcaagg ctcccgctca gggaggaaaa
     5761 gcgggggacg gcaagccgtc cggggagcaa tataacaaaa gcagcatggc gccgcaatga
     5821 tccggcgtac aaggcccttg ggccgccacg cggcgggcgc gcgtcgccac gcgggccacc
     5881 ttggggcaca attttcccca tgaagcacct gcccctcgcc ccctttcccg ccctccggcc
     5941 gcgccggctg cggcgcgacg acttcacccg ccgcctggtg cgcgagcacc ggctgtcggt
     6001 cgacgacctg atctacccgg tgttcgtgct ggacggcaag aaccggcgcg aagccgtgcc
     6061 ctccatgccc ggcgtcgaac gcctgtcgct cgatcagctg ctgccgctgg ccgaagaatg
     6121 cgtgcagctg ggcatcccgg tgctggcgct gtttccggtc atcgacgcca ggctgaaaga
     6181 cgacaagggc agcgaagccg ccaacgccaa agggctgatc ccgcgcgtgg tgcgcgagct
     6241 gaaaatgcgc ttccccggcc tgggcatcat gaccgacgtg gcgctcgacc cctacaccag
     6301 ccacggccag gacggctggc tggacaagac cggctatatc ctcaacgacg agaccgtggg
     6361 ccagctggtc aagcaggcgc tgacgcaggc ggcggccggc gtggacatcg tggcgcccag
     6421 cgacatgatg gacgggcgca tcggcgccat ccgcgacgcg ctggagaaaa aaggccacat
     6481 ccacacccgc atcatggcct acagcgccaa atacgccagc gcgttctacg gcccgtttcg
     6541 cgacgcggtc ggctcggcca agaacctggg cgccagcgac aagaaggtct accagatgga
     6601 ccccggcaac agcgacgagg cgctgcgcga agtcgccatc gacatcgccg aaggcgccga
     6661 catggtgatg gtcaagcccg gcctgcccta cctggacatc gtgcgccgcg tcaaggacca
     6721 gttcggcgtg cccaccttcg cctaccaggt cagcggcgaa tacgccatgc tcaaggctgc
     6781 ggcgcaaaac ggctggctgg accacgaccg ggtgatgatg gaaagcctgc tcgccttcaa
     6841 gcgcgccggc gccgacggcg tgctgaccta ctttgcccgc gatgccgccc gtttactaca
     6901 aaatcaatag ctactggcgc ttgtccatag cgcgccagaa gccattttga ccacgattcg
     6961 gagggggccg tcgtgcacat cgtcgaattc accggcggca gcctgcgctt tgccgacgcc
     7021 gtgccggcgg cggcgccggc cgacggcttc gtctggatct acctggaccg cgaggacttt
     7081 gcccgccacc tgcccgagct gcaagaggcg gcgcaccgcc tgggcggctc gccgctgctg
     7141 gacgtgcacg ccaaggacct ggccaacccg gcccaccctt cgggctacga cgccacctcg
     7201 gtgtacgacc tggtggtgtt ccgacgcctg gcgtcgctgg ccgaggtgca gctcaacggc
     7261 aaccacgacc cccacaccgg cgcggccggc gcgccgcgcg cgcccaagcc gcagccgttc
     7321 gagcgcatcg attcgcgcgc ggtcggcttc gtcgtcttcg accgcctgct gatcagcgtg
     7381 cacccgccgg gctgccagac ggcggtcggc tttctgcagc gccaggccga cgacgcgcgc
     7441 ctgagcgtgg acagcgccag cgcccgcaac cgcgtgccgc aggggccgga cgacctggtg
     7501 ctgcgcatgc tcaacgccat ggtcgacagc tacctggacc tgcggcgcga cctctccgaa
     7561 gcgctggagc aggcgcaggt cgagctgctc aagccgcacc cggcgccgca cacctggggc
     7621 gcgctgatgc acgcacgcgg gcgcctgcac gggctgcagg acctgtgcga ggagcagcag
     7681 gacgccatgc aggaatggct ggacaccttg cgcgagctgc cgctgtccac ctatggcccc
     7741 gacccgctgc tggcccagca gcgccacgac cagctggtgg cgcgcgcacg cgacgtcatg
     7801 gagcacatcg agcgcgtgct gcgccacgca cgccggctgg aacaggcggc cgaaaccgcg
     7861 gtgcagatcc actttgccgc ccagggtcag cgcaccaacg acaccatgcg tgcgctgacc
     7921 gccatcaccg cggtgttcct gccgctgaac ctgttcaccg gcttcttcgg catgaatttc
     7981 gaacacctgc cgctgatcca cagcgtggaa ggcatgtggc tggcgctgct gctgctggtg
     8041 gtgctggcgc tgggcatcgt ggtgctgttc tggcgccgcc gctacctgac gcgctgaggc
     8101 gcgcgctggc caggcaacac atggactcgc tcgacctgat ccagaccttc cgcgaagtcg
     8161 cgcggcgcgg cagcttttcc ggcgccgcgc gggcgctggg catgtcgccg gccaacgtca
     8221 gcaagtacat cgcgcagctg gaggcgcgct tcggcctgcg gctgtttcat cgcaccacgc
     8281 gcaaggtgac gctgaccgaa gccggcgagc tgctgttcga gcgcagcggc gcgctgctgg
     8341 agctgatcga cttcaccgag ggcgagatgc acgagcgcgc cacccgcccg gtcggccgcc
     8401 tggcgctgac cgtgccgcac gcgctgatgg ccagcgaact gccggccctg ctgggccgct
     8461 tcatgcagcg ctaccccgaa gtgacgctgc acctgcacgt caccaaccgc ctggtcaacc
     8521 tggtcgacga aggggtggac ctggcgtttc gcatcggccc gatcctgcag cccaacctga
     8581 tcgtgcgccg cctgctgcgg ctggaccacg tggccgccgc cacccccgac tactgggacc
     8641 ggcgcggtcg cccggcccac cccagcgagc tgctggagca cgaccagctg gccttcgccc
     8701 tgcccggcga agccccggtc tggcagttcc aggaccgcgg caagccactg gagctgcgcc
     8761 tgacgccgcg cttcaccgcc accgacccgg cgccgctcag aacctgggcg ctgcagggcc
     8821 tgggcgtggt ctggggcacg gtgcactggc tggccgacgc gctggccagc ggcacgctgg
     8881 agccgctgct gcacgactac tcaccccccg agatctggct gtacgtggcc tacatgcagc
     8941 ggcgccacag cagcgccgcc ctgcgcgccc tgctggacta cctggatgcg cagttccagc
     9001 cgccgcccac gcccacgctg ccggcgccgg ggcggcggcg ggtcgattcc cgatgacgga
     9061 tggggcgccg ccgcatcagc gcaggtgccg ggcgaagaag gccagcgtgc gctcgcgcgc
     9121 cagtgcggcg gcggcaggct gccaggcggc gcgctggtcg cagttgaagc cgtgcgcggc
     9181 agggtacagg tgcagctcga cctcggggtg cgcgcggcgc agcgccgcca cgccgtccag
     9241 cggcaccgaa gcatcgtggt ccgacagatg cgccagcacc ggcacgcgcg gctggcgcgc
     9301 cagctcggcc ggctgcgtca tgccgccgcc gtagtagggc accgccgccg ccagccggtc
     9361 cagcagggcg gcggcgcgcc agctgagcaa cccaccccag cagtagccga tcacgcccac
     9421 cttgccgccg ctggcctgcg ccgcgtggtc gatggcggcc tgcacgtcgg ccagcacgcc
     9481 gggggcgggc agcgcctcgg ccgccgcctt cagcgccagg ccggccttga cgtcggcctc
     9541 ctcgtagccc agctccaccc tggcgcgcaa gcgctggaac agggcgggcg ccagcgccag
     9601 atagccgtcg ccggcaaagc cgtcggcgac ggcgcggatg tggcggttga cgccgaagat
     9661 ttcctgcagc accaccacgg cgccgcgcgc cggccccgcg ggcggcgcca ggtaggccgg
     9721 caccagctgc ccgtcggcgg ccttcagctc gatcgtctgt cccatccgtt ggctcctgca
     9781 atcatcgtgt ggtttcggcc cttggatgcc ttgaaagttt caagaccccg cagccatggg
     9841 aacctggctg tttgccctgg gtgcaggcgc gccagcgccc gctccggctt gaagatcaac
     9901 ccgatgccgg caggccggcc agccagtcga tcagctggcg cagcgcctcg tcggtggcgt
     9961 cgcgcagcgc ctgggcgccg ccggcggcgt cgggcgtggg ggcgggccgg cgcacgctga
    10021 agctgcgctg gcccagcagc ggctgcgcgc gcgcgccggc ggcaagggcg gtcacgcgca
    10081 gccgcaccac gccttcgctg gtctgcgggc tgctgaactc gtgcgcgaag tcgtccagct
    10141 cgacgcgcag ctgcagcgcc gccagcgcgc tgccggcgtc cagcaccggg cgcgtggccg
    10201 ccagccgctg gcgcaggcgc agcgccagca gttgcggcgg cggcatgctc cagcgcgcct
    10261 gcgcatacgg gcgcggctgc tggccggcgc cggcgtacag caggcggtag accatggcct
    10321 ggctgtcgag ggcggggctg gcctcgatcg gcgccagcgc cagcggcgcg cccggcgtcg
    10381 cggcggcgcc ggccgccggc ggcggcccga ggtcgtaagg ctcggtgcgc ggcggcttgt
    10441 cgggcagcgg cagggcgcaa ccggccagcg ccagcgccgc caccgcagac aatgcaaaaa
    10501 aaacggccct ggcgcttgca ggacgtgcgc gagcagctat cgatttcata tcaatgctca
    10561 gcgtggagag gtcggatggg cgggcggcgg cacgaagccg gcttcgcccg ggccgggcgg
    10621 caccgggcca ctgccgtaga ccagcgcctg cgggttgtcg cccagggtgc gcgccacgcg
    10681 atcgacgcgg cgcaaggtct ggtcggcggc gtcggccaga ccgtgcaggc gcggcagcgt
    10741 gctggcgttg atggtctgca cgccctgatc cagccggtcg atcacgccgc ccggcgcggt
    10801 cacgcgcgcc acgccctgct gcgtctgttg cgccgcctgg cccagggtgt ggatcacggt
    10861 gcgcgcctcg gtggtggcgt cctgcagcgt cctgagggtg tcggtggcct gggtgatcag
    10921 gcgcggaatg ctggtgcggc tggtggccag ctgggcctgc agcgtctggt cggcggtgcg
    10981 cgccagccga tcgacgctgc gcgcggcggc gccgatgtcg cccagcgccc cggtcagcac
    11041 cgcctggttg tccggcgcca gcagctggtt gatgcgctgg gcgctggtgt cgagcttgcc
    11101 gatcaggtcc tgcgcgcgat cgccgagctg acccagcagc cccggcttga gcgggatgcg
    11161 cggcacgccg ttggggccgg gcggcagcgg cgcggcgtcg ctgccgtcgt cgtcgagctg
    11221 cacgaacgac aggccggtga cgccctggta ggccagctcg gccaccgtgg cgcgggtgat
    11281 cggggcgtcg ggcgcgatgg ccagcgtcac cagcacgcgg ccgcgctcgg cggggtcgaa
    11341 gctgatgcgc gtgaccttgc ccaccgccac gcccttgaag cgcaccgccg cctgcggctg
    11401 caggccgctg acggcgttgt ggctgacgat ctcgtaggcg cgggtgttgg tgacgtcgcg
    11461 cagcagccac atggccagcc cgatgagcag ggcggtgacc agcaccacga aggtgccggc
    11521 ggccagggcg tgggctttgt tttccatcga atcagtctcc ttgcgcgggc cgcgcgtgct
    11581 ggcgctggcg cagcagctcc atggcgcgcc ggccgcgctc acccaggaag aagtggcgga
    11641 tgaaggggtg ctcgaacgcc agcacctcgg gcaccggcgc gttgatgatg acgcgcttgt
    11701 cggccagcac ggccacgcgg ctggacagct cgaacagcgt gtccaggtcg tgcgtgacca
    11761 tcaccaccgt cagccccagc tcctggtgca gcgcgcgcag cagggcgacg aaggcgtccg
    11821 agctgtccgg gtccagcccg gcggtgggtt cgtccagcat cagcagcggc gggtccatga
    11881 tgagggcgcg cgccaaggcc acgcgcttga tcatgccgcc cgacagttcg gccggcatct
    11941 tgtgcgcgtc gctggccttc aggcccacca tctgcagctt cagcagtgcc gcttcgcgca
    12001 ccagcgccgg cggcagcgcg ccgctctcgc gcagcgcgaa ggcgatgttg tcgagcacgc
    12061 tgaaggccga aaacagcgcc ccctgctgaa acagcatgcc cacccgcgcc gcggcgccct
    12121 cgcgcgccag ctcgccggcc gggcgaccca gcaccgtggc ctggccacgc gccggccgcg
    12181 tcagccccag catgtggcgc agcagcaccg tcttgccggt gcccgagccg cccaccaggg
    12241 tcagcacctc gccgcgccgc accgtcagat cgaggtcctg gtgcaccacg tgctggccgc
    12301 cgccgggcag cgcaaacacg ctccacagcc cgcgcacctc gatcaccggc tggcccggcg
    12361 ccagcgcggt gtcgactgca tcggacgctt gcaccgccgc ctccatcagc cagcacccca
    12421 cggcgcacgc gctgcccgca tggcgcgacg catcgaagat cgaatggccg cgcagcaggc
    12481 caagccagta cagccgtggc cggacctgcg ccggccacgg ggtgcgtccc ccctcccaga
    12541 ggggaagacg cgcagcggtt caggggagtt cgtcatatcc ccacgctctt gaacagcacc
    12601 gcgaacagcg cgtcgatcag gatcaccgcc gtgatcgacg ccaccaccga ggccgtggtg
    12661 ccctggccca ggctttcggt gttgggcttg acgcgcaggc cgtagtggca gccgatcagc
    12721 gcgatcgaca ccccgaacac caccgacttg gacaccgcca gcgtcaggtt gggaatgcgc
    12781 accgccttgg gcagcgcctc cacgaagtac gccggcgtga tgcccagcgt gatgtccgac
    12841 gccagcatgc cgcccaccag cgccgccagc gtggtccaga ccgaaatcag cggcatcgcg
    12901 atggccagcg ccagcgcgcg cggcagcacc agccggaacc ccgccgggat gcccagcacg
    12961 cgcatggcgt ccagctcctc ggtcacgcgc atgacgccga tctgcgccgt gatggccgag
    13021 ccggaacgcc cggccagcaa caccgccgcc aggatcggtc ccagctcgcg gatcagcgcc
    13081 agccccagga tgttgacgat gaaggcctcg gcgccgaact ggcgcagcac ctggctcatc
    13141 aggtaggcca gcaccacgcc gatcagaaag cccaccagcg ccgtgatcgg cagcgcctgc
    13201 gcgccgatgc ggtacaggtg gccggaaaag tcgcgccacg gcccgcgccc gggccgcgcc
    13261 agcagccgca gcgcgtccag cagcagctgc cccatcaggg tcacgaagtc ccgcacctga
    13321 aaggcggcgt cggtcacgcg caggcccagc gcctccaccc cctcggacca gccggcgcgc
    13381 gcctcggccg gcggcgccgg cgcggcaccg gcggccacgc gctccagcag cgcgcgctgc
    13441 tcgtcgctgc tcagcagctc ggccggccag cggcggcccc agtggtgcca cagcaccagc
    13501 gcccccaggt gatcgagcgc cgccagctgg cgcaggtccc agctgccacc ggcggccacg
    13561 ccgcccagcg ccgcctgggt ggcgcgccag accggccgcg tgctcagcca tgccgccgtc
    13621 cactcgccgc gcagcagcac gcgcgccgcc gcgtcggcgg gcgcgtggtc gatgcggggc
    13681 ggttcggcgg acatggcggc aaggcgatcg gacagacact gtaacggccc cgcccgcacc
    13741 aggcaggtgg ccggcgacca cgcggcatgg gcgggccggc accgcgcccc cggggcgcct
    13801 ggttacgaat ctttcctctt gtagggcgtg ttggcgactt aggacattag gatcaattgt
    13861 ttaccacaag gagctagcat ggcacttcaa ggaccgttct tcggcaagcg tgacgaaccc
    13921 acctcccccc gcggccccgg cagtacgccg ctgagcggca ccagcgccct gggctcggcc
    13981 ggcgccgccg gcgcgcagcc gtttgcggcc ccctcggcgg cggccagccc ggccgccccc
    14041 gccagcaccc cggtggcggc ctcggtcacc gaaagcggcg ccaaactgat cgtcggcccc
    14101 aacatcaagc tcaagggcgt cgaaatcacc gattgcgaca cgctggtggt ggaaggcatg
    14161 gtcgaagcca ccatggactc gcgcgtgatc cagatcgccg agcagggctc gttcaagggc
    14221 tcggccgaga tcgacatcgc cgaagtgcgc ggcagcttcg acggcaacct gacggtgcgc
    14281 cagaagctgg tgatcttcgc caccggtaag gtcaccggca agatccgcta tggcaagatc
    14341 gtgatcgagg aaggcggaca actgtccggc gagatcagct ttggcaccga caaggatcac
    14401 aagagcgaca agcccgccgc cagcgcgggc agcagcaagg gcgagcagct cgccgtcgcc
    14461 tgagcgttgg cgccatcggg ccggcggcgc cgccgctggc ctgcccgccc cgcaggccgt
    14521 ccgcgcgacg gcctttttgc tggccgcggc cctggccttg ccgctgggcg gctgcgccgg
    14581 cagcgccccc accacgcgcg gcgtggtgcg cgggcaggac tttcagcccg ccgaactgct
    14641 gcagagcgaa ggcaaccgcg tggcacacaa cgccatgcag gccaaccagc gcagcctgct
    14701 gctgctggcc gacaagctgt accgccgcaa cccggccgaa tggcgcaagc gcgccagcac
    14761 gcgcgaggcg gcgctggccg acatcgaacg cagcctgctc accggccagc cctggccccc
    14821 gctgggcgac cggcgcgacg tggcggcgct gtcgtacgcg ctggcgcccg acttcatggg
    14881 cgaccgcgtg gccgccttcg tcatcgcctg cgccgacacc atcgtcaccg cgcacggcgg
    14941 caagcgcgag ttctactacc tcgacggcgt cgatccgcag catctctaca acgccgcacg
    15001 caacatggaa gtggcgctgt ggatcctcaa cagccggcgc gacgcccagg gccagccgct
    15061 gctgctggcc aacgacatcg gcccggaccg ccgcaacctg agcttcgagc gcgagttcgg
    15121 caagatcatc ggccgcctgg acctgctggc cagctacgcc accgaacgct accgccgcgc
    15181 cgtgatcggc tacgtgcagg gcgtggtggc ggcgccgctg ctggcctttc tgccggtccg
    15241 gtgacgccac ccgggcgccg cgcgctcctt tgtccttcgt catgggaagg ttgccgttct
    15301 gacggcacgc tcgggggcat cggcctgccg ccgcccgcgt ggggtgaccg acacgcccgt
    15361 gccccccatg ggttcctgcc gcgccgggct tgtggcatgc tgccagcgca ggagacatgg
    15421 catggacgaa ctgactttcg accacatcat cgtgggcgcc ggcacggccg gcagcctgct
    15481 ggccaaccgg ctgtcgcagg acgccggcaa gcgtgtgctg ctgatcgagg ccggcggcaa
    15541 ggacgactac cactggatcc atattccggt cggctacctg tactgcatcg gcaacccgcg
    15601 caccgactgg ctctaccaga ccgagccggc cgacggcctg aacgggcgca cgctgcgcta
    15661 cccgcgcggc aaggtgctgg gcggctgcag cagcatcaac ggcatgctgt acctgcgcgg
    15721 ccaggcgcgc gactacgacc actgggcggc gctgacgggc gaggacgagt ggcgctggga
    15781 gcattgcctg ccggacttca tggcacacga ggatcactat cgtcttgata gcgattcgcg
    15841 cagttccggt gcggtcggtg gcgccaatga ccaggattgg tcgggctttc acgggcacgg
    15901 cggggaatgg cgggtggagc gccagcggct gcgctggcag gtgctggacg cctttgccga
    15961 ggcggcggtg caggccggcc ttccggccag cgaggacttc aaccgcggca acaacgaggg
    16021 cgtgggctac tttcacgtca accagaagaa cggctggcgc tggaacaccg ccaaggcgtt
    16081 tctgcgcccg acctgctacg gtcgcagcaa tttcgagctg tggacgcgcg gccaggtgat
    16141 gcggctggcg ctggagcgcg acgccgacgg tgcgctgcgc tgcgccggcg ccgaagtctg
    16201 gaccggtggc gaaatgcaga aggcccgcct ggcgcccggc ggcgagctgc tgctggccgc
    16261 cggcagcatc ggctcgccgc agatcctgca gctgtcgggc atcggcccgg ccgcgctgct
    16321 gcagcagcac ggcattgcag tggtggccga cctgcccggc gtgggcgcca acctgcagga
    16381 ccacctgcag atccgcagcg tctatcgggt gcagggcgcg cagacgctga accagctggc
    16441 cagcacgctc tggggcaagg cgcgcatcgg cctgcagtac ctgtggtcgc gcagcggccc
    16501 gatgagcatg gcgcccagcc agctgggggc cttcacgcgc tcgtcgccgg cctacgcatg
    16561 gcccaacctc gaataccacg tgcagccgct cagcctggac gccttcggcg agccgctgca
    16621 ccgcttcccg gccatcaccg ccagcgtgtg caacctcaac cccaccagcc gcggcacggt
    16681 gcggatccgc agccaccgct tcgaggatgc gccggccatc gcccccaact acctgagcac
    16741 cgacgaagac cgccaggtgg cggccgacag cctgcgcgtg acgcgccgca tcatggccca
    16801 gccggccatg gcgcgctttg cgcccgaaga gtacaagccc ggcgtgcagt accagagcga
    16861 cgaagagctg gcgcgtctgg ccggcgacat cgccagcacc atctttcacc cggtgggcac
    16921 caccaagatg gggcgcgacg acgacccgat ggcggtgctc gacccccggc tgcgcgtgcg
    16981 cgacggtgcc ggcggcctga tccgcgcgct gcgcgtggtc gacgccggcg ccatgcccac
    17041 catcaccagc ggcaacacca acagtccaac gctgatgatg gccgaaaagg cggcgcgctg
    17101 gatccgcggc gcgtaagggg cgcaaggccc caccgcggtc gattggggca tgtttggggc
    17161 cctggcgccc gcccatggcg cgccatcagc tcttgttttt atagcgatcc aggccttgcc
    17221 gctggcccgg acacggacga ccgcgctgtg cttcgatgcc ccatgggaca tggtttggaa
    17281 cgcgattcag caccaggttc agatgccgtg gtagcggtcc ggtcggtggt tgatggcgat
    17341 gacgaagttg agcgccgccg ccgacagcac cgacagcgcc acccgccccg gcggcagcag
    17401 ccacagggcc gagcccagga tcagcgcgtc gcacagcatc tgcaccgtgc cggcgcgcca
    17461 gccgcggcgc tgctgcaggt agaaggccag cgcgcccagg ccgcccaggc tggcctggtg
    17521 gcgcatcagg atcaggatgc cggtgccggc cagcaggccg ctcatgacgg ccgagaacac
    17581 cgggtcgagc cgcgccagcc cgaccagccg cggcagcagc tcggtgtaga gcgccagcac
    17641 cgccaccgcc acgaaggttt tgagcgtgaa gcgccagccc agcgcgcgcc aggcgaacag
    17701 gtagaacggc aggttgagca cgaacagcgc cgcgcccagc gcccacccgc tggcgtagtg
    17761 cacgatgaag gccagcccgg tgaccccacc cggcagcagc cggctgtcgc gcatgaacac
    17821 cagcgccagg gcgacgaaca ggcagccggt caggatggcc tgcacgtctt ccagcgcgct
    17881 gtgggcgcgc ggcggcaccg acggccggcc ggcggcccgc gcgcgccgcg ccgcccagcc
    17941 ccgaagcccc tgccaccctt gaacgctgcg catggcggcg atgttagggc cgccagcgcc
    18001 ggccgccctt gcgccaggcc aagcttggct gccgccggcg gccgcttgcg gcgtgttggc
    18061 acccatggtg taatcgcaga ctttcgcgaa ggggagtagc tccctgggcc gcccggcccg
    18121 cctggtgcct tgcgcaccgg ccacgggcgg tttcgccggg tcgtcaatac gaagccttgc
    18181 ggcttccggc ccggcgggtg gcagaccgcg tcggccaccg agcaagacct tcgatcctgc
    18241 ccggcacgcg ccggggtcgg tcgaagctgc ggtcttgctg ccttgccccc aagacagtgc
    18301 ccgtgcggcg gccaccccgg caggtgccgc ttcggcgtcg cgccttgtcc ctgtctggag
    18361 ctgcaaccat ggaatttttc tctgccccct ggtggtccgc cttgctggcc atcgtcctga
    18421 tcgacctcgt gctggccggc gacaacgcca tcgtcatcgc gctggcggcg cgcaacctgc
    18481 cgcgcgagcg ccagcggcag gccatcgtct ggggcacggt gggcgccatc ggcgtgcgca
    18541 cgctgatgac gctggtggtg gtgtggctgc tgaagatccc gggcctgatg gcgctgggcg
    18601 ggctgggcct gctgtggatc gcctacaagc tggtggccga taccggcgcg gacgagagcg
    18661 agcacggccc ggcggccagc accttctggg gcgccatgaa gaccatcgtg atcgccgatg
    18721 cgctgatggg catcgacaac gtgctgggcg tggccggcgc ggcgcacggc gccatggacc
    18781 tggtggtgat cggcctgctg atcagcgtgc cgatcgtggt gttcggctcc accgtggtgc
    18841 tgcggctggt ggagcgcttt ccggtcatca tccagctggg cgcggcggtg ctggcgtgga
    18901 cggcggcgtc gatgatcgcc ggcgagccgc tgctggccgg ctggttcggc ccgcgcgagg
    18961 cgccggcgca ggcgctgcgc tgggcgctgg cggcgctgtg catcgtcggc gtgctgggcg
    19021 cggcctggct gatgcgcact cgccttcaga atgacccatg acccatgggc catggtctgg
    19081 aacgcgaagc cgtaggtgcg gccgcgctgc gctatgctgc gtcccatggc aaggatgatc
    19141 ctcaaatggt tgctcagcgc cgccgcgctg ctgggcgtgg cctacatcta tggcggcgtg
    19201 agcgtgagca gcttttccac cgcgctgctg gcggcgctgg tgatcggcct gctgaacatg
    19261 ctggtgcggc cggtgctggt gctgctgacc atcccgatca cgctgctgac gctggggctg
    19321 ttcctgttcg tcatcaacgc cctgatgttc tgggccgcct cgggcctggt gggcggcttc
    19381 aacgtcagcg gcttctgggc cgcgctgctg ggctcgctca tctattcggc gctggggctg
    19441 gtgatcgacg ccctgctgga gcgcgcctga acgctcgatt tgctattgtt tagatagctg
    19501 cctgcgcttg ccaggcaagc gtcggagcct tgttgaccgc ttgtcaactt cgggcagacc
    19561 gcaccgctgc gtgatctggc ggctgcgatg gctttcacct cttgggtgag cacgctacgc
    19621 acccgcccgc accgcgctcg ctgcgcttca gcggcgcgcc ttctgctctt cctgccgctg
    19681 ctgctgcagc agcgcctgcg cctggcgcag ccgggtgtcg acgatgctgg cttcgatcag
    19741 atcggcggcg cccgcggcgc ggcgcgccgc gtcctgcgcg gcgcgccagc ggtccacggc
    19801 gccggcgtag tccagctggg ccatgggtac ctcgccctcg gcgcgcaagg cgcgcagcgt
    19861 ctgcccctgg gccgcatggg cctgggccag ggcctgccag gcgccggcgt cctgcggctg
    19921 gtcgctgacc cagacctgca gcgcgtcggc cgcctgcgct gcctggccgc tggcggtctg
    19981 ggcctgggcg cgcagcagca gcgtggcgcg gccggcctgg ccgcggccgc tcggacgctc
    20041 gggcggcagc agttcgagcg cgcgcgccgg ctggtcggcc gccagcgcca gttcggcctg
    20101 cagcaggcgc gcctggcgcg ccgccgcggc gtcgccggcc accgcctgcg ccagctggct
    20161 ggccagcgcc tgcgcgcgcg gcagctcgcg cagctgcgcc cgcgccagcg cggcggcgta
    20221 cagcgcgccg gcgcgcgcgg ccagcgggcg ggcggcaaaa ccgggctggt cgggctcgcc
    20281 ggcccaggcg cgcagcacgt cgacgccggg gcggccgagc acgcgcgcac gcgccgccac
    20341 cagcagcgcc tccagctcgg gcgcggcgcc gcgcgcgacc gccggggcac gctgctgcat
    20401 gtcggcgatg cgctgggtgg tcagcgggtg gctgcgcagg tagggccagt cgccgttgtc
    20461 gttgatgcgg gtggcgttct gcagtttttc gaacatgccg acgaagccct ggccgtcgaa
    20521 gccggcctgc accagcacgc cgtagccgat gcggtcggcc tcgcgctcca tgtcgcgcga
    20581 gaagttgagc tgctgctgga tcaccgccgc ctgcccgccc accgccagcg cggcgccggc
    20641 ctgcgggttc cggctggcgg cgatcatgcc ggccaccatg gccgccagca tcagcggcgt
    20701 ctggcgcttt tcctgctcca gcaggcgcgg gatgtggcgc tgcgtgatgt gcgacagctc
    20761 gtgcgccagc accgacgcca gctcggcacg cgagccgacc acgccgacca gccccaggtg
    20821 cagaccgaag tagccgcccg gcagcgcaaa ggcgttgatg ctgcggtcgc gccccagcag
    20881 cacgcgccag gcgtagcgct cgtccagctc gggcggcagc tcgccgcgcg cccgggcgcc
    20941 gtcgagcagg gcgcgccaca ggccgtcgac gtattcgccg acgatcgggt cgtcgatgta
    21001 gtccgggtcg cggtacagct cgcggatgat gcggtcgccc agcttgcgct cttccagcgc
    21061 cgtcatttcg ccggcgtcgc cgagcgaagg caggccgcgt gcggccggcg ccggctgggc
    21121 cgcggccggg gtcgccagca gcagcgccgc cagcgcggcc gcggcgctcg cccgcaggcg
    21181 ccagctgctg aagcaggggg tggaggtggt ggtcatggtg cgtcgccgcg ccacgcggca
    21241 cggccggccg gcggcaaagc tggctctatg atgcccgcat tcgcgccgcg ttccccctca
    21301 gagtgttacc catggtctct cgcgcccccg cttccccgct cacgcatttc gacgcccagg
    21361 gccaggccca catggtggac gtcggtgcca agcctgccac gcaccgggtg gcggtggccg
    21421 gcgggcgcat cgagatgcag ccggcgacgc tggcgctgat cgaatcgggc aacgcgaaaa
    21481 agggcgacgt gctgggcatc gcgcgcctcg ccggcatcat ggcggccaag aagaccagcg
    21541 agctgattcc cctgtgccac ccgctggcgc tgtcccgtgt tgctatcgaa ttcgaagctt
    21601 ctggcgcaaa tgggacgggc cctgcggccg ttttttgcac cgccacggca gaaaccaccg
    21661 gccccaccgg ggtggagatg gaggcgctga ccgcggtgca ggtggcgctg ctgaccatct
    21721 acgacatgtg caaggcggcc gaccgcggca tgaccatcac cggcgtgcgc ctgctggaaa
    21781 agcgtggcgg caagtcgggc catttcgtcg ccgcgcgcgc ctgagccgcc catccgtgcc
    21841 gccttcgctc cgtcacgtcg cgcgggtgct gctgctggcg ctgccctggc tgtggccgtt
    21901 cacctccggc ccgacggcgg ccacccagcc ctacctggcg ggcgcggcgc tggcggcgct
    21961 gctgctgctg gcctggccgc gccagcgcca ggccggctgg cacgcgctgc tggacggctg
    22021 gctgctggcg gcgctggtca gcagcgccat cgccctgctg cagtacttca acctggaagc
    22081 gccgctgttt ccctgggtca accaggcgca gaccgggcag gccttcggca acctgcgcca
    22141 gcccaaccag ctggccaccc tgctggtgat cgggctgctg gcgctggcct ggcaggcgca
    22201 agccggccgg ctgagcccgc gagcggcggc agcgctgggc gtgccgctgc tgacggcgct
    22261 ggcggccagc gcctcgcgcg tgggcgcgct cgagctgctg ctgctgggcg cgctgtgcct
    22321 gtggtgggcg cgcggcagcg ggcagttgcg ccgcgcgggc gcgctgctgg cggcggcctg
    22381 gctgctgtac gcgctggcgg cgctggcgct gccgtgggcg ctgcagctga gcgagggcca
    22441 gcagggacgc gacctgatcg agcgcctgcg cgccggcgaa ggcacctgcg gcagccgcct
    22501 gatcctgtgg cgcaacgtgc tgcacctgat cgccctgcgg ccctggaccg gctggggctg
    22561 gggcgagctg gactgggcgc actacatcac cctgtacccg ggcgagcgct tctgtcacat
    22621 cctggaccac gcacacaacc tgccgctgca cctggcggtg gagctgggcc tgccgctgac
    22681 cctgctggcc gcgggcgtgc tggcctgggc ggtgtggcgc gcccggccct gggccgagcg
    22741 ccagccggcg cgccagctgg cctgggcggt gctggccgcc atcggcctgc acagcctggt
    22801 ggagtacccg ctgtggtatg cgccgttcca gatcgcggcg gcgtgttgcc tgtggctgct
    22861 gtgggccacg cggcgcggtg cgccggccga ggccgggccg gcgccgggcc ggcggctgct
    22921 ggcggtgctg ctgctggccg gcgtgggctg cgccggctgg gactaccacc gcatcagcca
    22981 gatctacctg ccgcgcgagc agcgcgccgc cgcctaccgc gacgacgcca tggcgcatgc
    23041 gcgccgctcc tggctgtttg ccgacgcggt gcgctttgcc gaggtgacca cccgaccgcc
    23101 gacgcgcgac aacgccgcct ggctgctgcc ggcggcgctg cagacgctgc attactcgcc
    23161 cgagccgcgc gtcatcaccc aggtgatcga aagtgccagc ctgctggggc gcgacgagct
    23221 ggcgctgacg cacctggcgc gctttcgcgc cgccttcccg cgcgaatacc gtcagtggtc
    23281 ggccgacaac cggcagctgc tggaggacgc gcaagcccag caacgcgccg ccggcagcgc
    23341 cccgcaggcg cccgcaagcg ctatgaacaa gtgagctgtc ggcgcaggat gggcgggcgc
    23401 tgcaggcata tttcgcactt ctacgacacg gccccgccgc cgggtcgcgc gtcatcctag
    23461 gaggctgtcg gacttggaaa tcgccggctg caaatcgacc gcagcggccc atttttcacc
    23521 ggctttttgc ccaatagctc gactattggg ctgcaaatcc ggcaaaaact ggtctcgctg
    23581 gggccgattc tcgctttcgg cgctccaagc ccgacagcct cctagaaacg acagttggat
    23641 gcacctgccc gtgccgtgat cggtgtgcca ggcaagggtg acgacgcagc gcactgctgt
    23701 gcgcaagaag gagccacgcc gcatggcgcg ccgagcgcgg tgcgggtgcg tgcgtagcgt
    23761 gctgacccaa gaagtgaaga ccatcgcagc cgccggtccc cgcttcgatg cggtctgccc
    23821 gaagttgaca agcgttcaac tgcggtttcc ggggtcatcc gccaggtggc gagcgcatgc
    23881 cgatcagccg aggccgctca gcgcgcgccg gtactgcacc gcctcggcca catgggccag
    23941 gctgaccgcc tcgctgccgg ccaggtcggc gatgctgcgc gccacccgga gcgcgcggtg
    24001 ggtggcgcgc gcgctccagc ccaggcgggc ggcggcgctg tgcaggaagg tgcgggcggc
    24061 ggcgtcgagc cgggcgtggg tgtcgatggc ctggccctgc agcgcctggt tgggctggcc
    24121 ctggcgcgcc agcgcgcgct cgcgcgcggc cacgcagcgc gcgcgcacgg cggcggtcga
    24181 ctcgccggcc ggcgcgttca gcagctcctc gggcgccagc gcgccgacct cgacgtgcag
    24241 gtcgatgcgg tcgagcagcg gccccgacag ccggccctgg tagcgcgcca cctggtcggg
    24301 cgtgcagcgg caggccttca ggcgtgagcc caggtagccg caggggcagg ggttcatggc
    24361 cgcgatgagc tgaaagcgcg ccgggaattc ggcccgctgg gcggcgcggc tgatggtgat
    24421 gtggcccgac tccagcggct cgcgcagcgc ctccagcgcg gcgcgcggaa attccggcag
    24481 ctcgtccaga aacagcacgc cctggtgcgc cagcgagatc tcgcccggcc gcggcggcga
    24541 acccccgccc accagcgcca ccgcgctggc cgaatggtgc ggcgcgcgcg tcggccgccg
    24601 gccccagtgc gccagccgga agctgccgtc caggctggcg atggcggcgc tctgcagcgc
    24661 ctcgtccacg tccataggcg gcagcaggcc ggcaaagcgc tgcgccagca tcgacttgcc
    24721 ggtgcccggc gggccgacca tgagcagcga atggccgccg gcggcggcga tttccagcgc
    24781 ccgccgcgcc gccgcctggc ccttgacgtc ggccaggtcg gggtaggccg gcgcggccgg
    24841 gcgcggcgac tcgggcacgc gcagccagcc ggcgtcgacc tcgtcctcgg ggccgaacgc
    24901 cgagtcttgc atcggcggct ggctgtgcgg cggctgaaag cgcgccacca cgtccagcag
    24961 gtggcgcgcc cggtacacca cctgaccggg caccagcgcc gcctcgccgg cgctgccggg
    25021 cggcagcacc agccgggtgt cgacgccggc ctgctggcgc gccacgctca gcgccagcgc
    25081 gccgcgcacc gggcgcagct cgcccgacag cgacagctcg ccggcaaatt cccagccggc
    25141 cagccggccg ccgtcgatct ggccgctggc cgccaggatg ccgagcgcga tcggcaggtc
    25201 gaagcgcccc gagtccttgg gcaggtcggc cggcgccagg ttgacggtga tgcgctggtt
    25261 gtgcggaaac tgcaggccac tgttgagcag cgcgctgcgc acgcgctcgc gcgcctcctt
    25321 gacctcgacc tcggccagcc ccaccagggt gaagctgggc aggccactgg ccaggtgcac
    25381 ctcgaccgtg acggcgggcg cttgcagccc catcagcgtg cggctctgta tcaggctcag
    25441 gcccatgcgg catctccccc aaagggtgcg tgcaccgact tgcggcaccc gcgacagaaa
    25501 aaaagcaccg ctttggtgca gaccgcccgg cgacgggcgc cgccacccga ggcgctggcc
    25561 tgaaatgtag cgggcgcgag ggctggcacg cggcgtgctt ggtgtgccat gtcccatttt
    25621 tttttgatgt caccggagag caaggatatg caccccaccc tgtcacccct gaccccccgc
    25681 cgcctcgccc ttggcctgct gtgcgccagc gccgccggcg cctgggccca gagcagcggc
    25741 gccgcggcgg cggccacgcc tgagcacagc ctcagcttca acgtcggcct gaccagcgac
    25801 taccgctacc gcggcatctc gcagagccgg ctcaagccgg ccgtcagcgc cggcgccgac
    25861 tacagccacg ccagcggcct gtacgccggc gcctggctga cgtcgatcaa gtggatcaag
    25921 gacgccggcg gcgacgccgc ggccgagctc gacctgtacg ccggctacaa gggcagcgcc
    25981 ggcccgctgg cctacgacgt gggcgtgctg cgctaccagt acccgcgcgc ccacctggcc
    26041 gtcagcccca acaccaccga gatctacggc gcgctcagct acggcgtgtt cacggccaag
    26101 tattcgcatg cgctgaccaa cctgttcggc tttgccgaca gcaagggcag cggctatctg
    26161 gacctgtcgg ccaacctcga cctgggcgac ggctggtcgg tggtgccgca cgtcgggcgc
    26221 cagtgggtgc gccacaacag cgccttctcc tacaccgact acgccgtcac gctgaacaag
    26281 gacttcggac acggcctggt ggccagcgcc gccgtggtgg gcagcaacgc cgacaaggcg
    26341 ctgtacgtga cgccgtccgg gcgcttcacc ggtcgcaccg cgctggtgct cggcctgaag
    26401 tacacgttct gattcaaaac cgccgctggt gcttgtccat tcagcgccag cagctatcaa
    26461 aaaggaagca tgatgaaact ggtcactgcc atcatcaaac ccttcaagct cgacgaggtg
    26521 cgcgaggcgc tgtcggcact gggcgtgcag ggcatcaccg tcaccgaggt caagggcttc
    26581 ggccgccaga agggccacac cgagctgtac cgcggcgccg agtacgtgat cgactttctg
    26641 cccaaggtga agctggaaac cgcggtcgcc gacgatcagg tcgagcgcgt catcgaggcc
    26701 atcgaagcgg ccgcacgcac cggaaagatc ggcgacggca agatttttgt cgccgcgctg
    26761 gaacaagtcg tccgcatccg caccggcgaa accggtacgg acgcactgtg aacccctgca
    26821 aggacatggc catgaaacac cccattgctc ccctcaccct ggccttcggg ctggcgctgg
    26881 ccctgccggg cgccgcctgg gcccaggccg ccagcgacgc cgccgccgcc gcgccggcac
    26941 tggccgcttc ggccgctgcc gccgcttcgg ccgcggcggc ggcggcgccg gcacccaaga
    27001 tcgacaccgg cgacaccgcc tggatgctga cctccaccct gctggtgatc ctgatgacca
    27061 tccccggcct ggcgctgttc tacggcggcc tgggccgcgc caagaacatg ctgagcgtgc
    27121 tggtgcaggt gttcgtcatc ttcgcgctgg tcagcgtgct gtgggcgctg tacggctaca
    27181 gcctggcctt cagcggcgag ggcaatttct tcggcgggct ggacaagctg ttcctcaagg
    27241 gcgtgacgcc cgagaccttc ggcgccctga ccaccatccc cgagtacgtg ttcctggcct
    27301 tccagggcac ctttgccgcc atcacggtgg cgctgatcgt cggcgccttc gccgagcggg
    27361 cgcgcttttc tgccgtgctg ctgtttgccg tgctgtggtt caccttcagc tacgtgccga
    27421 tggcgcacat cgtctggggc ggcggcctgc tggccaagga cggcgcgctg gactttgccg
    27481 gcggcaccgt ggtgcacatc aacgccggca tcgccgggct ggtgggcgcc tacctggtgg
    27541 gcaagcgcat cggcttcggc cgcgaggcgc tggcgccgca cagcctgacg ctgaccatgg
    27601 tgggcgcctc gctgctgtgg gtgggctggt tcggcttcaa cgccggctcg gccggtgccg
    27661 ccaacgccat cgccggtctg gccttcgtca acaccgtgct ggccacggcg gccgccacgc
    27721 tgagctggat cgcgctggag gcgatgcaca agggcaaggc ctcgatgctg ggcgcggcct
    27781 cgggcgcggt ggccggcctg gtggccgtga cgccggcggc cggcttcgtc ggcccgatgg
    27841 gcgccatcgt catgggcgtg atcgccggcg cctgcggcct gtggggcgtc agcggcctca
    27901 aacgcctgct gcgggtggac gacgccttcg acgtgttcgg cgtgcacggc gtgtgcggca
    27961 tcgtcggcgc catcctgacc ggcgtgttcg ccgcgcaggg cctgggcggc accggcggcc
    28021 tgacgcccga cagcttcagc atgggcgcgc aggtgtggat ccagatcaag agcgtgctgt
    28081 tcaccatcgt ctggtcggcc gtggtgtcgc tgatcgcctt caagatcgcc gacctggtgc
    28141 tgggcctgcg cgtcagcgag gaagacgagc gccagggcct ggacatcagc agccacggcg
    28201 agaccgccta cagccgctga cggcgcccgc ttctccaagg tcttcatggc ccgcccgccg
    28261 gcgggccttt ttttcgtccc gggcgcggcc cgcgcagcgg gctgccgcta ccatgacggc
    28321 atggaagccg cattgcagtc cctggccgca aagatcacgc aggccgccgc gcgcggccgc
    28381 ccgctgcgca tccgcggcgg tggcaccaag gatttctatg gccagcagct gcacggcgag
    28441 ctgctggacg cgcgcccgct ggccggcgtg ctggcctacg aaccgagcga gctggtggtc
    28501 accgtggggg ccggcacgcg cctgcgcgag ctggaagcgc tgctggccga agccggccag
    28561 cacctgccct tcgagccacc acgctttggc gtcgccggca ccgtcggcgg cgcggtggcg
    28621 gccgggctgt cggggccggc acgcgccagc gtcggcgcgg cgcgcgactt cgtgctgggc
    28681 gcgcaactga tcaacggccg aggcgagcgg ctgaacttcg gcggccaggt gatgaagaac
    28741 gtggccggct acgacatcag ccggctgctg gccggctcgc tgggcaccct ggggctgatc
    28801 acccagctca gcctgaaggt gctgccgcgc gcgccggccg aggccacgct ggtgttcgcg
    28861 ctgtcggaaa ccagcgcccg ccagcagctc aaccgctggt gcggtcagcc gctgccgctg
    28921 agcgccagcg cctgggccga cggccagctg tgggtgcgcc tgcgcggcgc ccaggcggcg
    28981 ctggccagcg cctgccggca gatgggcggc gagcggctgg acgagccgcg cgcccagcgc
    29041 cagtgggacg cgctgcgcga ccagacgctg ccgttctttc gcctggcgcc ggacgaggcg
    29101 ctgtggcgcg tgtcggtgcc cgataccgcc agcccgctca acctggggcc gacgctggtc
    29161 gagtggcatg gcgcgcagcg ctggctgcgc ctgccgccag ccgaggcgcc gcgcgtgcgc
    29221 gaggtggcgg cgcggctggg cggccacgcc acgctgtttc gcggcggcga cggcgtcacc
    29281 ccggtgttca ccccgctgac gccgccgctg gcgcgcatac accaggcgct caagcgcgag
    29341 ttcgacccgg ccggcatctt ctgtcccggc cgcctgaccc cggatttctg atgcagaccg
    29401 cactggcccc cgaataccaa ggcaccgcac tcggccagga ggccgaggcc atcctgcgca
    29461 aatgcgtgca ctgcggcttc tgcaccgcca cctgcccgac ctaccagctg ctgggcgacg
    29521 agttggacgg cccgcgcggg cgcatctacc tgatcaagca ggtgctggaa ggccacgccc
    29581 cgacgcgcga cacccagctg cacctggacc gctgcctgac ctgcctgaac tgcgagtcca
    29641 cctgccccag cggcgtgcag tacggccacc tgatcgacat cggccgccag gtggtggaag
    29701 ccaaggtggc gcgcccgccg gccgaggcgc gcacccgcgc cctgctgcgc cgcggcctga
    29761 gctcgccgct gttcggcccc gccatgcgcc tggggcagtc gctgcgcggc ctgctgccgg
    29821 ccacgctgcg cgccaaggtg ccggcgccgc ggccggccgg cgcctggccg cgccgcacgc
    29881 acgcgcgcaa ggtgctgctg ctggaaggct gcgtgcagcc gtccatggcg ccgggcatca
    29941 acgccgccac cgcgcgcgtg ctggacgccg ccggcatcga atgcgtgctg gcgcgcggcc
    30001 agggctgctg tggcgccatc aagtaccacc tggccgacgg cgccggcggg ctggcgcaga
    30061 tgcgcgccaa catcgacgcc tggtggccac acatcgagcg cggcgcggtc gaggccatcg
    30121 tcatcaacgc ctccggctgc ggcgccatgg tcaaggacta cggtcacctg ctgcgccacg
    30181 agccaccgta tgccgagcgc gccgcgcgcg tatcggccat ggcgcgcgac gtcagcgagc
    30241 tgctgcccga tctggtggcg gcgctgcgcg gccgcgtgcg cccgccggcc gaggtgctgg
    30301 cctggcacgc cccgtgcacg ctgcagcatg gccagcggct gcgcggcggc gtcgagcagc
    30361 acctgggcgc gctgggcttt cggctgcgcc cggcggcgca ggaggcgcac ctgtgctgcg
    30421 gctcggccgg cacctattcg gtgctgcagc ccgagctgtc gctgcggctg cgcgagcgca
    30481 agctgggcca gctgctggat gagggcgcgg ccgaccagcc ggcggccatc ctgtcggcca
    30541 acatcggctg catcacccac ctgcaggccg gcaccgaggt gccggtgcgg cactggatcg
    30601 agctgctcga ccaggcccta catgctactt aattgatagc tgttggcgct tgtctgtcgg
    30661 gcgccagagc cgttttttgc tcttgtctca agcgccgcac caaggattcg caccaaggat
    30721 tcgcccatga ccggcattca ggactggacc ctgccctacg cctcgcgccg ctcgccggtg
    30781 ctggggcggc aggtggtcag cacctcgcag ccgctggccg cccaggccgg gctgcgcatg
    30841 ctgctggccg gcggcaacgc ggtggacgca gcgctggccg ccgccatggc gctgacggtg
    30901 gtcgaaccca gcggttgcgg catcggcagc gacgccttcg ccatcgtctg ggacggccag
    30961 gccctgcacg gcctgaacgc ttcgggccgc gcgccccggg cctggacgcc ggactacttt
    31021 gcgcgcctgg gcggcatccc cgacaagggc tggaacgccg tcacggtgcc gggcgcggtg
    31081 tcgggctgga tcgcgctgtg gcgccgcttc gggcggctgc cgctggcgca gctggcgcag
    31141 ccggcgatcg actacgcgcg ccacggcttt ccggtctcgc ccaccatcgc caccctgtgg
    31201 acgctgggcg ccgagcggct gggcgcccag cccggctttg ccgaatgctt tctgcccgcc
    31261 ggccggccgc cgcgggccgg cgagctgttc cgcagcgagg cccatgcgcg cacgctggag
    31321 gacatcgtcg ccaccgaggg cgagtcgttc taccgtggcg cgctggccgc gcgcatggcc
    31381 gcccacgcgc gcgcctgcgg cggcgccatg accgaggacg acctgggcgc gcaccaggcc
    31441 gattgggtgg gcacgctggc gcagcgcttc ggcgactcgg tggtgcacga gatcccgccc
    31501 aacggccagg gcatcgccgc cctgatggcg ctgggcatgc tggacgagct gggcatcgga
    31561 gacgcgccgg tggacagcgc cgacagcctg cacctgcaga tcgaagccat gaagctggcg
    31621 ctggccgaca cctaccacta caacgccgac ctcgcgcaca tggcggtggc ccccgcgcag
    31681 ctgctggagc gcggctacct gcgccagcgc gccgccctga tcgaccgcca gcgcgccggc
    31741 gagccgacct ggggcacgcc gcgccccggc ggcacggtgt acctggcggc ggccgacgcc
    31801 cagggcatga tggtgtcctt catccagtcc aactacatgg gcttcggctc cggcgtggtg
    31861 gtgcccgcca ccggcatcag cctgcagaac cgcggccacg gcttcagcag cgccgccggc
    31921 cacgccaacc aggtggggcc gggcaagcgc ccctcgcaca ccatcatccc ggccttcgcc
    31981 atgcacgccg acggccggcc gcagatggcc ttcggcgtca tgggcgggcc gatgcagcac
    32041 cagggccatg tgcagatggc gctgcgcgtg ctgcgccacg gccagaaccc gcaggccgcc
    32101 gccgacgcgc cgcgctggcg cgtcaccggc gggcgcggcg tggcgctgga gccggcggtg
    32161 gacgccggcg tggtgcaggc gctgcgcgcg cgcgggcacg acgtccgcgt ggaaagcggg
    32221 cacggcgtgt tcgccttcgg cggtgcccag ctggtgctgc gccaggacgg gcactacgtg
    32281 gccggctccg acccgcgcaa ggacggcggc gcagtggcgt tctgaggcac ggcgcgccgg
    32341 cggcgtgcaa aagtcacgtc ggcgcggcgc gaccgccacg ccgaggccga gcaagcacgc
    32401 attggccgcc tgggtgaggc gcaccgcccc agcagtgccg ccacgccgcg ccgcggcgcc
    32461 ccacaatccc gccgcccgcc accgtggcgg gcccaacccc tgcatcgcca ccgcatgcgc
    32521 cacccctaca ccctgatcgg tctgacgccg tccgaacgcg ccctgctgga gtcgctgttc
    32581 gccctcgagc gggacaccag cggggccatg gtgccgacgc cggatccgcg cgaggcccac
    32641 ctgatcgtgg tcaacggcga cgaccacacc ctggtcgagc aactgcgcca ggacaacccg
    32701 cgggcgctga tcgtgctggc cggccacccc gcgggcgcgc cggtcaccgg actgccggtg
    32761 ctgcgccgcc cgttcgacat gccgggcgtg atcgaagtgc tgagcggcct ggactggccg
    32821 gccgggctgc cgtgcgtggc ggcgggcgag ttcgaccgca ccttcgtctc ttcggcgccg
    32881 accagcagcc agctcgacac cggcgccccg ccggcggtcg acccccaggc gtttgcgccc
    32941 accaccgctt cggcgccgct ggcgcctgcg ccgcaaggtc gcgccgtagc cgtacccgac
    33001 cgggccccgg tgtcctcgcg cgccgcctgg gcccgcagcg agctggcgcc gccgccctgg
    33061 agcgccgcag cagccgctgc gccaggcggc ttcgtcgacg accgcgaggc cgaggtgctg
    33121 gtggtcaccg gcgcactcgg cgagcgctcg ctcaccctgc cgcgtggcat ccggcgcctg
    33181 ggcttgcggg tgcggctgct ggacggcccc gcggccgtcc tggcggccct ggaccagggc
    33241 gcggcatcct ttgtcttcct cgaccagctg tcgctgggcg agcagctgct gccgctggcg
    33301 cgcgcgctgg cggcgcggcg tgccggcaac ggtgcgccgc cgcacgtggt ggtggtggcg
    33361 cggcgcggca ccccgctcga tcgcctgcgc gcccgcctgg ccggctgcca ctggatgcag
    33421 gtgccgatcg acgggccgcg gctggtggcc ttcttcgcgc gccgcggcct gaggccgcgc
    33481 gacggcgtcg ggcctgcatt cagctcaacg cccgatgacc aatgacgcat gacgccttcg
    33541 tcatcgaagc gcagcgaggt cattcgtcat tgggcatggc tcagggcttg agcgccaggc
    33601 cgcaccaccg tcaacccgct cgcccacgcg gcatcggcag gatcgacagc agcgccgcca
    33661 gcaggatcag cacgccgccg gcccaggtcc agcgggtggg cgtggcggcg cccagcagca
    33721 ccgcggaggc gctggcgaac accacctcgg ccagcatgat gagcgccgtc acccgtgccg
    33781 gcagccgggc ggcgccgtac tgcagggcca ggttgccggc cagaaaggcc agcgtcaggc
    33841 ccagcaccca gggcagccag gccagcccgt gcgtgctcag ggccagcggc gacaggccgc
    33901 cggccaggcc ggccagcgcc agtccggtgg cggtggcgaa accgccgccg aacatggcca
    33961 gcacgcgcgc cggcggcggg gtggcgcgcc agcgccgcag cagcacgttg gtgagcgcaa
    34021 aacccgcccc gcccagcagg gccagctggt cggccgcgcc gcgcggccac ggccagggcg
    34081 cgccgggccg atgcagcacc agcaccagcc cggccagggc gagcaccagc cgcagcagcg
    34141 ccgcgcgcgt gggccgctcg cccagcagcc accaggccag accgaccgac cacaccggca
    34201 tcaggtagaa cagcagggtc acgcgcacca cgtcgcccag cgtcaccgcc cagttgaagc
    34261 cgatgttggt cacccccgcc gcggccgcca gcaccagcag ttgcgggtgg cgcgcgaagg
    34321 cgcgcaggct ggccggctgc caggccagca gcgccagcaa cgccacgccg aacaccaccg
    34381 ccgtgctcca cagcgggtgc aggccgtgct ggtgcagggc cttgaaggcg atccaggaca
    34441 gaccccaaac cagcgcgttg cccagcaggg ccagcgccgg cagcagggcc gacggcggat
    34501 gggcggcggc catgggaggc atgccgggga tcaccctgca cgcggccggc gcagccgaac
    34561 gccgctggcg tgatcgacgt cccaggccgg gcgcggcacg cgccgagcat gcgggcgcgc
    34621 cgcctgccca cccctcaggc aaaggcccgc caggccgcaa gttcgcctgt gcatacggat
    34681 ttcaggaaga tgacaagcct caacggccgc tgtgaggttc aatgtccgcg atcgcgccgg
    34741 gcgatgttca gcaggcgttc gaccttgccc gggtagcggc gcaggttgtg gcggtgccag
    34801 cgctggatca gcagcatgaa ggccatcacc acgccaccga acagggcaat ggcgccgaac
    34861 gccgacaggc cggcgccggt ggaagcgctg tagagcgcgc ccagccccag gatgcaggcc
    34921 tgctcgttga agttctgcac cgcgatcgag cgcccggccc ccatcaggtt gtggccgcgg
    34981 tgctgcagca gcgcgttcat cggcaccacg atgtagccgc ccagtgcgcc catcaggatc
    35041 agcaacggca ccgccagcca cagccggtcg atgaacacca tcagcaccac cagcgcaccc
    35101 atcaggatgc ccagcggcat caccgcgatg gcgcggtcca gccgcacgcg cagcgaagcc
    35161 agcaccgccc cggcggcggt gccgatggcc accacgccca ccaggccggc ggcctgggtg
    35221 gtgctgtagt gcagggcggc ggcggcccag gccagcacga tgtagcgcat gttgcccgac
    35281 acgccccaga acagcgtggt ggtggccagc gagatctggc ccagcttgtc gcgccacagg
    35341 cgcgcgttgc agcgccagaa gtcgggcagc agcgacagcg tgcgcgccgg caacgggcgc
    35401 atgcgcacgc cggtgtgggg gatgcgcagg ttgatggcgg cggcgatggc gtacaccagc
    35461 accaggctgg cgatcgccgc ctcggccggc gtgtcgatgc cgctgtgcag gcccggcaga
    35521 tccagcccca gcaggcgcgg cgccagctgc gggccgacca gctgtccgcc cagcagcacg
    35581 cccaggatga tggaggcgat cgtcaggccc tcgatccagc cgttggcgcg caccagctgc
    35641 gaaggcggca gcagctcggt caggatgccg tacttggccg gcgaatacgc cgccgcgccc
    35701 agcccgacga tggcgtaggc cagcagcggg tgaccgccga acagcatggc caggcagccg
    35761 acgatcttga tcgcgttgct gaggaacatg acgcgcccct tgggacgcga atcggcgaac
    35821 gcgcccaccc agggcgccag caccacatag aacagcgcga acatcggcac cagcgccgcg
    35881 cgctgccatt cggccgcacc actgccgcgc agcagttcga cggcggcgac gaacagggcg
    35941 ttgtcggcga gcgagctgaa gaactgcgcc gccatgatgg tgtagaagcc ccgtctcatg
    36001 ggcgctgggc cggacacgcc gggccgtggc agatccgaac caaagaagta agctcctgta
    36061 tcggtgcgcg gttatagcac gcgccggcgg tgccagaatc gcgctccatc gtccccagcc
    36121 accctgtcat gccccgcccg atccaagcca ccatccacac cggcgcgctg cgccacaacc
    36181 tcgaacgcct gcgccaggcc gcgcccggtg cccaactgtg ggcggtggtc aaggccaacg
    36241 cctacggcca cggcatcgag cgcgtgttcg aaggcctgcg cggcgccgac ggctttgccc
    36301 tgctcgatct ggccgaggcc gaacgggtgc gcgcgctggg ctggcgcggc ccgatcctgc
    36361 tgctggaagg cgtgttcgag ccgcgcgacc tggagctgtg ctcgcgcctg tcgctgtggc
    36421 acgcggtgca cgacgacgcc cagatcgact ggctggccat gcacaagacg caccagccgc
    36481 accatgtgtt cctgaagctg aactcgggca tgaaccggct gggcttcgcg cccgagcgcc
    36541 tgcgcgccgc ccacgcgcgg ctgtcggcgc tgccgcaggt gggcgagatt tcactgatga
    36601 cccatttcag cgacgccgat ggcgcgcgcg gcatcgccgc ccagtgggcg gtgttccagc
    36661 gcgtcaccga gggcctgccg ggcgagcgca gcgtctgcaa cagcgccgcc accctgcgcc
    36721 acgcgcagga cgcggcggtg cgcgccgact gggtgcgccc cggcatcgcc gcctatggcg
    36781 gctcgcccga ctttccggcg cacgatgcgg cccactgggg cctgcagccg gccatgacgc
    36841 tgcgctcgcg gctgatcgcc accggcagcc tgcaggccgg cgacagcatc ggctacggct
    36901 ccaccttcac cgccgagcgg ccgatgcgcg tcggcgtggt cgcctgcggc tacgccgacg
    36961 gctacccgcg ccacgccccc accggcacgc cggtgctggt cgatggcgtg cgcacccgcc
    37021 tgctggggcg cgtcagcatg gacatgctga gcgtcgatct ggagcccgtg ccacaggccg
    37081 gccccggcag cgcggtgacg ctgtggggcc agggcctgcc gatcgacgag gtggcgcacg
    37141 ccgccggcac catcggctac gagctgatgt gcgcgctggc gccgcgcgtg ccggtgcagg
    37201 tcgactgagc ccgaatggta ttcaagacat cccaaggccg catccatcga cgcgatcggc
    37261 gatcagaaca ggcgccaacg cccatccggc aagcgccagg agctctgatt tgaatagcgt
    37321 tcgtggcacc cgtggcagcg gcgccgccag tcagcgcagg aaggcgtcgt agccggtctt
    37381 gaggatcagc gcccccacca ccacgatgaa caccccgcgc acgaagccgg cgccgtgcct
    37441 gagcgccagc cgggtgccga tcaggctgcc ggtgacgttg gcggcggcca tcagcagccc
    37501 cacctgccac cagacgtggc ccttggcggc gaacagggcg atggcggcga cgttggtggc
    37561 gacgttgagc agcttggcgg cggccgaggc gtgcagaaag tcgtagccca gcaggcgcac
    37621 gaacaggaag atgaagaaac tgccggtgcc ggggccgaag aagccgtcgt agccgccgat
    37681 cacggcgccg atggcgcagg ccagcagggt ctcgcgcccg cgctcatggc gcggcgcgtg
    37741 ggtgcggccc aggtccttct tcgccagcgt gtacagcagc accgccagca ggatcagcgg
    37801 cagggcgcgg cgcaggaagg tggggtcgat caacgtcacc gcccaggcgc ccagaaacga
    37861 acctgccagc gccgccagcg ccgccggccc cagcaccgcc cagcgcatct gcacgcgccg
    37921 ggcgtactgg aaggtggcga tgccggtgcc ccagaccgaa gcactcttgt tggtgccgaa
    37981 cagcgtggcc ggggcggcgt tcgggtagac cgcgaacagc gccggcacca gcaccagccc
    38041 gccgccgccg acgatggcgt cgatgccgcc ggccagcagc gaggccagcg agacgaggag
    38101 caggtccatg aatgcccgaa gaactattga tttgatagct tttggcgcag gtctggcaag
    38161 cgccagaggc caaaaccgtg tgaattatcg tatgggttcg gccatgggct gtcttgagct
    38221 ccagattccg aggccagagg gcatccgtca accgggcaag aaaaaagggc gccacatggg
    38281 cgccctgcaa gtcggtgttg acggaaatga tgatctgttg tggtggtctt tgggtttgtc
    38341 tcctcggccc ttcggcgtgc gcacacgcct gagtgcccgc actgtaagcg accgcccggc
    38401 ggccggccat tggtgcttgc ccgtagggga tttccgccaa ggcaggactg gtgagcaatt
    38461 gctcactttc gtgatgcgct gctgtcgtgg ccgagctggg gcagcatcga gccttcgccc
    38521 agctgcaaca ggccgctgcg gctgtagacc gccagcttgc cgcgcgtgtc ggtgatgtcg
    38581 aggttgcgca tggtcagctg gccgatgcgg tcgagcgggg tgaagggcgc gttttcgacc
    38641 ttttccatgc tcaggcgctc gggcgcgtag gtcaggttgg gcgactcggt gttgaggatg
    38701 ctgtagtcgt tgccgcggcg cagctccagc gtgacttcgc cggtgacggc gccggccacc
    38761 cagcgctggg cggtttcgcg cagcatgatg gcctgcgggt cgaaccagcg gccctggtag
    38821 agcaggcggc ccaggcgcag gccgttgatg cggtactgct cgatggtgtc ttcgttgtgg
    38881 atgccggtga ccaggcgctc gtaggcgatg tgcagcagcg ccatgccggg cgcttcgtag
    38941 atgccgcggc tcttggcttc gatgatgcgg ttttcgatct ggtcgcacat gcccaggccg
    39001 tggcgcccgc cgatgcggtt ggcttccagg aacagctcca ccgggtcggc gatctcgcgg
    39061 ccgttgagcg ccaccggccg cccttcctcg aagcgcacgc gcacctcttc ggccttgacc
    39121 tcgacctcgg gcttccagaa cgccacgccc atgatcgggt tgacgatgcg gatgccgctg
    39181 gacagctctt ccaggtcctt cgcctcgtgg gtcgcgccga gcatgttgga atcggtcgaa
    39241 taggcctttt ctgccgacat cttgtagtca aaaccggcct tctgcatgaa ttccgacatt
    39301 tccttgcggc cgcccagctc gtcgatgaag cgctggtcga gccagggctt gtagatcttc
    39361 agcgccgggt tcgccagcaa cccgtagcgg tagaagcgtt cgatgtcatt acccttgtat
    39421 gtgctgccat cgccccagat gttgacgtca tcttccttca tcgcgttgac cagcatggtg
    39481 ccggtcacgg cccgcccgag cggcgtggtg ttgtagtagg tggcgccggc ggtctggatg
    39541 tggaaggcgc cgcactgcag ggcggcgatg ccttcgttgg ccagctgggt gcggcagtcc
    39601 accagacggg ctttttcggc accgtattcg agcgccttgc gcgggatggc gtcgtagtcg
    39661 gactcgtcgg gctggcccag gttggcggtg taggcgtagg gcacggcgcc tttctggcgc
    39721 atccacagca gggcggccga ggtgtccagg ccgcccgaga aggcgatgcc gaccttctgg
    39781 ccggcgggaa gcgattgcag gatggtgtgg gacatgggct ggggcggcgg gcgggcgccc
    39841 gcgcggcggc tgcgggggtt ggaaaaagcg cgattgtccc gcctttttgg cgcgcgccgc
    39901 cggcggcgcc tgccggcggt ccggtgcgtt gcggccaaat cggcctctga cgcacaacca
    39961 gcgagcgctg gcagctacgc tttcaatagc gtatggcggc gccgctcaac cgtccaggcg
    40021 ctggcgcagg gcgatcacct cttccatcag gtcggccgtc agggcggcca gctgcgggtc
    40081 ggcgtcgtac acctgctcca gctgggcgat gcggcgcgcg cggcgcagac tgaggctggc
    40141 gaagcgccac tcggtgctgg cgccgccgcc cagcggctgc agcacgccgg cttgcacatg
    40201 ggcgtgcacc cattcggggc tcatggcgca ggcgcgcgcc agttcgtgca ggctgagcgc
    40261 ggcgtcgtcc agcagctcga ccagctcggc ggggatggcg gtggggcggg aaggcatggc
    40321 ggtcatcctc tgcgtggccg cggggcgtgc ccgcccatcg ggcgcaggcc agccaggcgg
    40381 cgaaatcgga acatcatgcg ggtctgtaac aggtgacggg tgatcgacgg tcgttcagcg
    40441 agcggtgcgg ccgcgttcag gcgccgcgcg ggtcgaagcc ggggtaggcc tcggccagcc
    40501 gcgcccaggc cgccttctgg gcgtcggtga cggcgccggg cacggccagt tcgagctcca
    40561 gcagcagatc gcccggcgcg ctggccgagg ggatgccgcg gcctttcagg cgcagcttgc
    40621 ggccgctgcc ggagccggcc ggcacgccca cttccaccgt cttgccatcg ggcgtggcga
    40681 cctgcaccgc gccgcccagg gcggcttccc agggcgtcac gcgcagccgc tgggtgacgt
    40741 cgcggccatc gatgcgccag cgcgcatcgg gccggaactg cacttccagg aacagatcgc
    40801 cggccggccc gccgtgccag cccggctcgc cctgaccggc cagtcggatc agctggcctg
    40861 gcttgacgcc ctgcgggatg cgcacctgca gcgtgcgctc ctgcggccgc acctggccct
    40921 gggcgtcgag gtgcgcgccg cgcaggtgga tggcgcgttc ggcaccgcgg taggcgtcga
    40981 tcagttccag ctcgatgcgc gcatggtggt cctggccgcg cagcggcgcc tgcgcgccgg
    41041 cgcccggccg ggcaccgcgg gcaccggcac gggcgcggcc gaacagctgc tcgaagaatt
    41101 cgctgaattc gccgccctcg ccgtgcgggc cgaagccggg cgcggcgccg tcggaaaact
    41161 cgtagccggt gttccagccc ggcggcgggc gcacgtcgtc gcccgagcgc gcgccagccg
    41221 cccaggcctg cgcgcccacg ctgtcgtagg cggcgcgctt ttcggggtcg gacagcacgg
    41281 tgtgggcttc gttgacctcg gccatgcgcc gggcggcgtc gggttccttg ctgacgtcgg
    41341 ggtggtagcg gcgcgccagc ttgcgatagg ccttcttgat gtcctccggg ctggcgctgt
    41401 ggctgacgcc caggatctgg tagtagtcct tgaactgcat gcgtttgcct gtgtgtgcca
    41461 gggcggccac cgaaaccggg ccacccgtcg gcatttatat gagggtccgg ccccgggctg
    41521 caagcggcgc cgggcgggca tcccgcatgc tatgcttaat atagctaccg gcgcttgttg
    41581 cgcctgcgcc ggtgaccgat ttgaaaaccg gctcagtcgg agcgcgccgg cgcgcggcgg
    41641 ttgaccagca gcatgcccag cgccaccagc gccagcgcca ccaccaggct gacggtgatc
    41701 ggctcgccca gccacagcgc gccggccatg agggcgaaga tcggcgtcag aaaggaaaac
    41761 gccgacatcc gcgtggccgg gtaatgcgcc agcagccaca tccaggccag gtagctggcg
    41821 aaggcgccca ccaccgtctg cagcgccagc gagctggcgg caaacgcgct ccagcgccag
    41881 cgccagggct cgcccagcgc cagcgacagc agcggcagca ccacggcgct gacggccacc
    41941 tggtagaaca gcatcttctc gggcgccacg cgcaccagcc gggtggcacg gatgctgacc
    42001 gtggtcagcc cccacagggc accggccgcc agcgccagca ggtcgccgcg ccaggtcggc
    42061 ccggcgccgc cctgcgcaaa gccttcgcgc agcgcgaaca gcaccgccgc gaaggcgcag
    42121 gccaggccca gccactgcag gcggtgcagg cgctcggccg gcaccagcca gggcagcacc
    42181 agcgcgaccc agaacggcga ggtatagaga aacaccgtca gccgcgaggc ggtggtgtat
    42241 tgcaggccca ggtacatggc ggcgaattcg ccggcgaaca gcgccccggc cagcaggccc
    42301 gcgcccaacg tgccgtcgcg ctggaacagc ggcacgccgc gccagcggca ccacagccac
    42361 agcagcgcgg tggcgccgac gaagcgcagc gcggcctgga acaccggcgc cagctcgggc
    42421 agcgtggcct tgaccatcac ctgctgcacg ccccagaaca ggcagcagac cagcaggatg
    42481 gcgatcgccc gcccgtccag gtgctcgcgg cgcgcgctca tggcaagccc tgcgcgctga
    42541 cgcgctgcgg gattcgcgct tgggagcggc ccggcgcgct tattcggtgt ccctccgcgc
    42601 tgacgcgctg cgggattcgc gcttgggagc ggcccggcgc gctcatgcgc cgggccctgc
    42661 ggcggcgctc acagcggatg ctccgtgtag aaccggccgc cgtgaaacag cagcggcgcg
    42721 gcgcccgggc ggtgcgcgca atgctccacc tggccgatga acagcacgtg gtcgccctcg
    42781 tcgtggcggc tgcggttgaa gcattcgaaa tgcgccaccg cgccttcgat cagcggcgcc
    42841 ccggccaccc cggggtgcca ggccacgccg gcccagcggt cgcccaccgg cgccgcaaag
    42901 cgcagcgcca gctcctgctg ctcggccgcc agcacgttga tggcgtagtg cgagccgcgg
    42961 cgaaacgcgt gcagcgacag cgagcgcagg cccaggctcc aggccaccag gggcggatcg
    43021 agcgagaccg agttgaagga attggccgtc agccccacca gcgcgccgtc ggcgctgcgc
    43081 gcgctgacga tggtcacgcc ggtggcgaac atgcccagcg cggcgcgaaa ttcctgcgcg
    43141 gaaaaagcgg gctggtgggc gcgaagggcg tggggcatgg gcgggcggcg tgggcagccg
    43201 gccatgatag cgaggcggcc ggcgccgcgt gtgcgccggg gtgatcgccc tggcccggca
    43261 cccgccggcg gcgtaccatg gccccatggc gctgctgccc tccttcgcca ccctgggtca
    43321 cggcccgccg gtcgtgctgc tgcatggttc gggcggcggt gcccgcagct tcgcgccgca
    43381 gctcgagtcg ctggccagcc tgggctttcg cgccatcgcc tgggacatgc cgggctacgg
    43441 ccagagcgca ccgatcgagc cctacggctt caaggggctg gccgagcgtg gcgcggcgct
    43501 gatcgaggcg ctggcgccgc tgaccggcgg cgcgccggtg gccctggtcg gccacggcct
    43561 gggcggcatg gtggcgcagg agctggtgct gcgccgcccc gatctggtgc gccagctggt
    43621 gctggtggcc agcgtcgctg cggtgtcggc cgacgacggc tacggccgcc acgtggcgca
    43681 ggcgctggcc tggctggacg aggggcgcga catgggctgg atcgccgccg agcgcgtgcc
    43741 ccggctgatc ggcccggggg cgctggccag cggcgtgcgg ctggccaccc actgccaggc
    43801 caccgtgccc gccccgacct ggcgacgtgc cctgctggcg ctggccggct tcgaccgccg
    43861 cgccgcactg gcgcacattg ccgtgccgac gctcctggtg gcgggcgagc tcgatccgct
    43921 ggcgccgccg ccggtgctgc gcgccatggc ggcgcagatc accggcgcgc acagcgaacg
    43981 gctggcacac accggccacc tgccgcagct ggagcgcccg caggcgttcg accgcctgct
    44041 gctgcccttt ctgcgccacg cgcgggcctg gcggcactga attttgctat taagataata
    44101 gctgtcggcg cttgcctgga gcgcgccaga gcccgattca actcagtatc ggttcagcgc
    44161 accagcagca ccggcacttc gcagctggcc agcaccttgg tggccaccga gcccatcacc
    44221 aggccaccca gcgcgccgtg gccgcgcgag cccatgacga tgaggtcgaa tttgccgctt
    44281 tcggccgtct tggcgatcaa ctcgccggca ttgccgacct tggacagcgt cttcacgttc
    44341 agctggtggc gggtcaggaa cttgaccacc gggtccagca ccttggccgc ttcttcggcg
    44401 tagtacgtct ccacgatctc gcgtcccacc gccgcgcgcg cccgcggcgg cagcgcgccg
    44461 tgcaccgtca gcgcggtgta ttcgatgtcc ccggaaaaca ggtccaggtg cgtggtcagg
    44521 taggcgagca tcttcttggt gaaggcgctg ccgtcgacgg caagcagaat cttcatcgcg
    44581 cggtcctcga aattgcgggt tggggggcgg acagtgtaag cgccccctgg cgggggccgt
    44641 ccatgacggc gctcaagcct cgcgctggcg cgccggcgcc acgctcaccc aggcgtcggg
    44701 gacgaagccg tgccgctcgc ccttgctcgc gcagggcctg ctgacgctat gcaaggggac
    44761 cgcgttgctt cgccaaggga agtgctgctc gccccgcccc ggcagcgccg agccgcgctg
    44821 gcaggcaccg acgtcgcccg ccagcaccag caggtcggcc accggcgccg gccgcggtac
    44881 gacgtcgggc tggcgctccg ggtgcaggtc gggccgcagt tggatgttca cgacggacgg
    44941 cgcagtcgat ggcgcgccgc cggtgtgaca cgaacccctt cccgccatgg ggtcttggtg
    45001 taaaccctta ggggttaacc ctaggtgcga ctacaatttc tccatcggcc aaaggccttg
    45061 ctggagaacg cccgacgtga cccgaacggc gtgtgatgac catgtggaat gtctggatcc
    45121 gccatctgac gaccctgttc gccccgctca ccagggcgcc ccggcacggc ccgggggtgc
    45181 ccgccagctt gctggagcgc gccgacgcct gtgccgggcg tgacccccgg ggcgccgccg
    45241 acctgcgcgc cgcggccctg aactatctgg gtgtggtgcg ctgagcctca cccgtcatcg
    45301 cgaaccaagg aacccaccat gagcaccacc agcctgaccc tcggccgcac ggccgtccgt
    45361 gaaccgcagt tcgaacccgc ccgcctggac cactggctgc cgcgcccgca gaccggcggc
    45421 ccgagcatga gccagctgct gcgcgcacgc tggaaccagc tgcgccagcg cctggcgcag
    45481 gcgcgcagcg aacgcgaaat ccaggccctg gccgacctcg accaccgcgt gcagcgcgac
    45541 ctgcagacgg cgcgcgacct ggccgaatgg aaagtgccgc accgcccgtt cgtctgaacc
    45601 gcaccggcaa ggtggctgcg ccgcctgggc cgaccaacga aaagccgccc gcgggcggct
    45661 tttttgcgtg ccggtgcgac acggcgtcaa gccatcacgc ctgctgaccc ctgacccaag
    45721 ggtgaggggt cgggcggggc gccgtcaggc cgcctccagc cgctgcgcca gcgcctgtcg
    45781 ggtggccagc agctcgggcg gcaggccctg ggccagcttg gtgaacagct cgtcgtgcag
    45841 ggcgaattcc tgtgcccaga cgtcggcgtc gatgcgggtg acggtgtcga actgggcggg
    45901 gctgaagctc aggccgctcc agttgatctc gtcatggcgc ggggcgacgc caaacatggt
    45961 gggctgaccg gcggcgcggc cttcgacgcg gtcgatcatc cacttgagca cgcgcatgtt
    46021 ctcgccgtag ccgggccaga cgaacttgcc ggcggcgtcc ttgcggaacc agttgacgca
    46081 gtagatggcg ggcaggcgcg cgccggcggc ctgcagccgc tggcccagct tcagccagtg
    46141 gccgaagtag tcggccatgt tgtagccgca gaagggcagc atggcgaacg ggtcgcgccg
    46201 caccacgccc tgggcgccaa aggcggcggc ggtggtctcg gagcccatgg tgg
//
LOCUS       DAOQTZ010000010        35705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1380_length_35705_cov_13.734306, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000010 DAOQTZ010000000
VERSION     DAOQTZ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 35705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35705
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_35705_cov_13.734306"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKN51_02445"
     CDS             <1..226
                     /locus_tag="PKN51_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNT83989.1"
                     /translation="EVVFYDGKAISVELPTSVEREITWTEPAVKGDTSGKVLKPAKIA
                     TGFEVAVPLFVNQGDRIEIDTRTGEYRRRV"
     gene            496..1368
                     /locus_tag="PKN51_02450"
     CDS             496..1368
                     /locus_tag="PKN51_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNT83990.1"
                     /translation="METTQDIKTSSLTSIQDAWATLQAHAQNGTPLEPAAYRLAFADP
                     EFLLRPETRGVRFQLELLKPDLDLSAAGVHSTVVVYGSARFVAPDEAAQQLASARTSG
                     DAAALAAAELAVRNAVYYAQAREFARLVATHSERQRAEDRLFICTGGGPGIMEAANRG
                     AHDVGMPSVGLNIALPHEQHTNAYVTPYLAFKFHYFALRKMHFMMRAKALVVFPGGFG
                     TLDELFEVLTLVQTRKARPVPIVLCGSSYWSRLLNLDMLVEEGAIAPEDLQLYRVVDT
                     PAQAWDAIQSFYRL"
     gene            complement(1391..2035)
                     /gene="lolA"
                     /locus_tag="PKN51_02455"
     CDS             complement(1391..2035)
                     /gene="lolA"
                     /locus_tag="PKN51_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNT83991.1"
                     /translation="MHVPRLSAVALVWFALGAPVARADGLQNLDAFIRHSRSGSADFT
                     QTVTAPAKPGQPARVKTSSGRFEFQRPSRFRFDYQKPFVQTIVADGKTLWLYDADLNQ
                     VTQRAQAQVLGQTPAALIAAAPDLRALQAEFTLAAAPDRDGLQWVSATPKQRDGQLQQ
                     VLVGFDGERLAALEIVDSLGQRSVLKFGRIEVNPALPASTFEFKPPAGADVLRQ"
     gene            complement(2488..3135)
                     /gene="pth"
                     /locus_tag="PKN51_02460"
     CDS             complement(2488..3135)
                     /gene="pth"
                     /locus_tag="PKN51_02460"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNT83992.1"
                     /translation="MIKLFVGLGNPGPEYAATRHNAGFWWIDQVARQLGVRLVPERAF
                     HGLVARASVQGASVWLLQPQTFMNLSGKSVAALARFYKVAPQEILVAHDELDLPAGEA
                     KLKQGGGHAGHNGLRDIHAQLGSSDYWRLRLGIGHPGNRQEVVGWVLKKPPLDEQIGI
                     EQAIDRAARAFALLAAGDMTAATQAIHTSRPPRPKPPRKPPPSPADAGASERPDV"
     gene            complement(3250..3867)
                     /locus_tag="PKN51_02465"
     CDS             complement(3250..3867)
                     /locus_tag="PKN51_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNT83993.1"
                     /translation="MKFVAFERSKQGTGASRRLRNAGKVPGIVYGGEGEPQLIELDHN
                     ALWHAIKKEAFHSSVLDMELGGKTAKVLLRDVQVHPYKQLVLHVDFQRVSAKQKIHMK
                     VPLHYRGEEESNAVKLDKCLINHILNELEISCLPADLPEAIEVDLSKLEKGATVTLKD
                     IALPKGVEPVLRGHSAEDTVLVSVVTPAAEAAEGEAPAAAEGDKK"
     gene            complement(3962..4939)
                     /locus_tag="PKN51_02470"
     CDS             complement(3962..4939)
                     /locus_tag="PKN51_02470"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNT83994.1"
                     /translation="MQPSDPSDFLVFTGNANPTLAAEIASSLGISLGAADVGRFSDGE
                     VTVEIKQNVRARDVFVVQSTCAPTNDNLMELLIMVDALKRASAERISAVIPYFGYARQ
                     DRRPRSTRVPISAKVVANMLQAVGVNSVLTMDLHADQIQGFFDIPVDNIYASPVLLGD
                     LRQQNYEDLLVVSPDVGGVVRARALAKQLNCDLAIIDKRRPKANESEVMHVIGDIEGR
                     NCVVMDDMIDTAGTLVKAAEVLKQRGAKKVYAYCTHPIFSGPAIERIAKGSALDEVVV
                     TNTIPLSAAAAACPKIRQLSVAPLIAQTIQRIARGESVMRLFQEQENLF"
     gene            complement(5026..5102)
                     /locus_tag="PKN51_02475"
     tRNA            complement(5026..5102)
                     /locus_tag="PKN51_02475"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5066..5068),aa:Gln,seq:ttg)
     gene            complement(5142..5999)
                     /gene="ispE"
                     /locus_tag="PKN51_02480"
     CDS             complement(5142..5999)
                     /gene="ispE"
                     /locus_tag="PKN51_02480"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNT83995.1"
                     /translation="MRAFYDLPAPAKLNLFLHVTGRRADGYHLLQSAFMLIDWCDTLH
                     VERRAGGALSRQDLGPTLPADDLILRAARALQQASGCAEGAHIMVHKRIPAQAGLGGG
                     SSDAATTLLALNRLWRLGLNRAQLARIGLALGADVPFFLHGTHGWVEGIGERIRTLQL
                     PPARFVVVKPPQGLETAAIFRDPALKRDSKPAIIAGFAADPYGFGHNDLQPVAERLCP
                     QVGQGLALLAQHGLHGRMTGSGSAVFAPVVPGAMTGVTEVQVPPDWGLRWCSNLEFHP
                     LGGWAPGEV"
     gene            complement(6014..6463)
                     /locus_tag="PKN51_02485"
     CDS             complement(6014..6463)
                     /locus_tag="PKN51_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNT83996.1"
                     /translation="MIAGCAHQTGQNAPFDSQTSHWSGRLGLTVASEPPQSYSAGFAL
                     SGNALVGELMLTSPLGSALAVMRWQPGQALLQQGEQTARFESLDALVEQVTGTPLPVR
                     ALFGWLHGQPQSVPGWQADLSGLPDGRLFAQRLMPLPTAELRLVLDR"
     gene            complement(6508..8157)
                     /locus_tag="PKN51_02490"
     CDS             complement(6508..8157)
                     /locus_tag="PKN51_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT83997.1"
                     /translation="MPETSRLNARLLYELLLAELLAREGEAQQGAAYMLNAARRTGDE
                     ALFKRATELAIQSRAGPAALEATRAWRQAFPDSAQAGQYELQVLIVLGRVAETEEPTR
                     HFLATLPEAERLSFITALPALYQRVPDKAEAARVIERALDGALSQPALAPAAWTSIGR
                     LRLQAGDKAGALAAATLGQKADASSEWPALLGLQLMGSGETQAEALVERHLSTAQARP
                     ELFIAYARALIEQGRGGDAHRQLDRLIARAPEHPEAWLVRGALYADERKDAEAEADLR
                     RYLSLLEATPATPDAEPPEGSAHARMMLAGIAERRGELTQAQQWLASVDSPEQALAVQ
                     TRLAQILARQGRLEEARQSIRAAPERSPEDARLKLLAEAQLLRDHGQPGQAYELLNGA
                     LQQTPDEETLLYEAAMAAERVGRFEVMERLLRRLIELKPGASHAYNALGYALADRNLR
                     LPEARTLIEKAVQLSPNDAYIQDSLGWVQFRQGNLRQARQTLEAAFRKRPDAEIAAHL
                     GEVLWILGEREAALRVWREGQRLEADNETLRKTLLRLRVRL"
     gene            8316..9131
                     /gene="mutM"
                     /locus_tag="PKN51_02495"
     CDS             8316..9131
                     /gene="mutM"
                     /locus_tag="PKN51_02495"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNT83998.1"
                     /translation="MPELPEVEVTRLSFADRIRDARILAARPGLPLRWPLGCAPEQLV
                     DRTVQDVRRRGKYLLLHLDQGVLLLHLGMSGSLSFDAAPAAPGRHDHFDLVTTHGTLR
                     LHDPRRFGAVVWSASLQLDPARKLLASLGMEPLGERFDPAAFHAGLRGRRAPIKQVLL
                     AGDVVVGVGNIYASEALFQAGIRPTVRADRLSRARADRLHAAVRQVLARAVERGGSTL
                     RDFSSATGESGHFQLEAAVYGRAGQPCLQCATPVRLIRQGQRSTYFCPRCQRA"
     gene            9282..11201
                     /locus_tag="PKN51_02500"
     CDS             9282..11201
                     /locus_tag="PKN51_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNT83999.1"
                     /translation="MSSRLKNLAAWLRQQRLLDDATDESLQRLVEQLRSDKVMVAFVA
                     EFSRGKSELINAVFFAGYGRRIMPASAGRTTMCPTELAYDAELPPCLRLLPIETRLQP
                     QALMEWRMAPEQWTRVDLDVNDPQQLASAMEKVSEVRYVDQAQARALGFWHDDRPEDN
                     PPVNAQGLVEVPRWRHALINMAHPLLRQGLVVLDTPGLNAIGAEPELTVNLIPQAQAV
                     VFILAADTGVTKSDLGIWREHVATMQDSSSSRFVVLNKIDALWDELSTPAQIEAQIAR
                     QCADTARTLALQDDQVLALSAQKGLLAKVRGDAALLADSRLPHFEDLLGQQVLGARHA
                     MLHDAIGAGVARAQAEAERTLAMRGRELSDQTIELNGLRGKNHQVIHQMRLRIEQEKA
                     DFDRSAASVLAVRSVHLKLLREVFQLLGNASIKRELAELNAALREPGLKLGIKKSYAD
                     GFERLRGVIRQVQALEGEIHAMLKAAFVQLNTDYGFALQAAEPPAMERFVSDLDAVER
                     SHLRYLGVGHMLQLARAGFSEKLVRALLTRVRSIFETALGDIELWNKATTSQLDTQLR
                     ERRRAYGRRIDAVERIQDAAGSLDQRIHDLQQEQQALQATAQRLRELGHELRRASATD
                     AADTAPPELTEVVPA"
     gene            11198..12301
                     /gene="mutY"
                     /locus_tag="PKN51_02505"
     CDS             11198..12301
                     /gene="mutY"
                     /locus_tag="PKN51_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNT84000.1"
                     /translation="MNGGGAELAARVMAWQRRQGRHDLPWQGTRDPYRVWLSEIMLQQ
                     TQVTTVLAYYPRFLARFPDVERLAAASEDEVLGLWSGLGYYRRARHLHRCARDVVAHH
                     GGRFPASAAELARLPGIGRSTAAAIAAFCFGERAAILDGNVKRVLTRALGFGHDLALA
                     RHEQALWVQAEALLPRTDLRRAMPVYTQGLMDLGASVCHRRRPDCPRCPLHDLCVAAA
                     AGQPERYPVKRRRVARQRLELWLLLARDAQGRVWLQQRPEHGIWARLFCLPLFDDRAT
                     LLASLPARVHGQAVTEPPRRHVLTHRDLDLHPVSVRLRATDLRHADGRWFGADEWPAL
                     GLPAPVRRLLAAPAAAPQAPRGRLGEVRPSVQK"
     gene            complement(12344..13588)
                     /locus_tag="PKN51_02510"
     CDS             complement(12344..13588)
                     /locus_tag="PKN51_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HNT84001.1"
                     /translation="MQFDALAARAQAFLTELTDFPVRPTAHTLWQRFRQDRLGLTASA
                     LTFTTVLALVPFVAVALSVFTAFPAFGQLQQALQQWLIDSLIPASIADSVMGYLTQFA
                     GQASELGLLGFAGLTLVSLSLMLTIDKTLNQIWRSSQTRPLSQRILVYWTALTLGPLV
                     MGASLAISSYVVTASRGWLPDLPGSVDAALALLQLLLMVGSLTLLYRLVPNTPVRWRH
                     ALAGALFVSVCLGLARQGLGWYLTRMPTYSVIYGTLATLPILLLWIYLGWLIVLFGAV
                     IAAYLPSLLSGVARRGGGPGWDFRLALELLQALAAARDGAGRGLTLPQLAQALRVDAL
                     QLSAAMAALTGLDWVGQLGTRSENEEPRHVLLIEPRRQLLRPLVERLLLAPDEALAGW
                     WARGLRSEATVADVLSPAATDS"
     gene            13611..14006
                     /locus_tag="PKN51_02515"
     CDS             13611..14006
                     /locus_tag="PKN51_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNT84002.1"
                     /translation="MPAHPSPAVQATRVVAVASLLALMALGLAWELWLAPLRPGGSWL
                     ALKVLPLALALPGLLRYRMQTCRGLALFVWLYFVEGVVRATSDHGVSARCALAQVALV
                     LLLFAACAAQVRLRQRAARAAAAGPAVSH"
     gene            14067..15494
                     /locus_tag="PKN51_02520"
     CDS             14067..15494
                     /locus_tag="PKN51_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNT84003.1"
                     /translation="MTTDTPLLDLLRQTVGAPHVLTEGDLTAYEQDWRRRVRGRALAV
                     VRPGSTAEVAAVVRACAAHGVAIVPQSGNTGLSVGSVPDDSGTQLLLNLGRLDRVRAI
                     DRANLTLTAEAGCVLQRLQAAAQDAGLLYPLSLGSEGSCMLGGNLGANAGGTQVLRYG
                     NTRELCLGLEVVTAQGEVWDGLSGLRKDNTGYDLRDLFIGSEGTLGIITAATMKLYPR
                     PAATLTAWAAVPSLAAALELLGLAQRHLGAGLTGFEVMGQFALGLVDRHLPQLRVPLW
                     RDTPWCVLLENADQESQAHARARFEPLLEAALDAGCVSDAVVAESIAQARALWHIRES
                     IPLAQAEEGLNIKHDIAVPVSRVPDFVAETEALLARQIPGVRLVVFGHLGDGNLHFNV
                     QAPEGGDAAQFLRQQEERVNLLVFDAVARHQGSISAEHGIGSLKAALLPRYKSPVALT
                     LMRAIKQALDPQGLMNPGRVLARDR"
     gene            complement(15430..16029)
                     /locus_tag="PKN51_02525"
     CDS             complement(15430..16029)
                     /locus_tag="PKN51_02525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT84004.1"
                     /translation="MGADASRWRIAPLAGADVPALLQVQAACYGPALLEDAAVYARRL
                     ASPAHCGLGLHAGGALAAYLAAHWSVWGAVTPLHGDFAPQAAADTLYLHDLAVRPEHA
                     GQGLGQRLLAAAWRQARARGVRQAALVSVQGTREFWQRQGFVVALRQPPALASYGSQA
                     LYMTRRLDDAAEPGSTGPINGRAPARAPGSSGPAGPAPA"
     gene            complement(16033..17655)
                     /locus_tag="PKN51_02530"
     CDS             complement(16033..17655)
                     /locus_tag="PKN51_02530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT84005.1"
                     /translation="MASFEENHVLTLDSQAPQATENAASDNPFQALGLAPELLAAVAE
                     LGYSAPTDVQRQAIPLALQAHPEGANDLMVSSQTGSGKTAAFLLPVLHTLLDMQREAQ
                     AREQAAWDARVAEALARGEPAPRKPRRKNPLNPRQFKPAVPGALVLCPTRELAQQVAR
                     DAIDLVRHCRGLRIANVVGGMPYGQQIAQLQNAALVVATPGRLLDLQRSGQIVLDQVR
                     FLVVDEADRMLDLGFADALAEIHQLTAQRQQTMMFSATFAAPIQQLALRVMHDGGARV
                     RKVQVATPQQSHANIRQELYWADSPEHRRRLLDYWLRDPSIDQAIVFACTQIECDQLA
                     ADLQQSGFAAVALHGALSQALRNRRLRALREGKVQILVATDVAARGIDVPSITHVINH
                     GLPMKAEDYTHRIGRTGRAGRDGLAVTLAEFRDRRRLAEIEAYTRQPFRPLVVAGLEP
                     TRHFPPVSEPRAPRRPAAHDGGARRAGGPRGAARPTRDRQAEFSPAARLAGAGSYQNK
                     SSPGASARPRAAAGRARAGGAPRPYTPGRAGR"
     gene            18122..19438
                     /gene="fahA"
                     /locus_tag="PKN51_02535"
     CDS             18122..19438
                     /gene="fahA"
                     /locus_tag="PKN51_02535"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNT84006.1"
                     /translation="MSTLNDTHDPHLTSWVTSANDGRTDFPIQNLPFAIFRRRGGSEP
                     WRGGVAIGDQIVDLAAAAQAGLFHGEAQAAAQAGAEPSLNRLMSMGHQAASSLRKALS
                     KALQSGSPQQAALQPCLVPQAEAEYDLPCRIGDYTDFYTSVHHATNIGKQFRPDNPLL
                     PNYKWVPIGYHGRSSSIGVSGQAFHRPRGQVKPPDADAPSLKPSARMDIELELGVFIG
                     ASNALGAPVALDEAEAHVFGLVLFNDWSARDIQPWEYQPLGPFLSKNFASTISPWVVT
                     MEALAPFRTPFTRPADDPQPLPYLASPAHSAAGGLDIELEALIQTARMRAAGSPPTRI
                     THTNYRHAYWTAAQMVAHHTVNGCNLQPGDLLGTGTLSGPTLDQACALIELTTGGKHP
                     IALPNGETRSWLEDGDTIVLRGWCARPGAARIGFGECVGTVLPARD"
     gene            19539..20753
                     /gene="dinB"
                     /locus_tag="PKN51_02540"
     CDS             19539..20753
                     /gene="dinB"
                     /locus_tag="PKN51_02540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNT84007.1"
                     /translation="MDAFYASVEWLRYPQLKGLPMVIGGGRRREDELIARLNRQRPER
                     VWTAADLAEIPVDCFPLLRDYVGRGVITTASYAARQFGIGSAMGLMKAARLCPQAILL
                     PADFDEYRRCSRAFKRVILDIAPVMEDRGIDEVYIDFTQVPGGQREGGRVLARLLQKS
                     IADATGLSCSIGVAPNKLLAKMASEFDKPGGISIVRDSDLQSLIWPLPCRKINGIGPK
                     TDARLQQHGIHRIGDLAARERAWLIAQFGASHGAWLHDAAWGRDERPVVTDSEPVSLS
                     RETTFERDLHAVHDRAELGAVFTRLAEAVAADLQRKGYAGKTIGIKLRFDDFRIATRD
                     QTVAEPTQDARRIRQIAGQCLKRVELSRRLRLLGVRVGSLVRADRLSAASDEASNHDL
                     PLFQQAPAAPEN"
     gene            20850..21149
                     /locus_tag="PKN51_02545"
     CDS             20850..21149
                     /locus_tag="PKN51_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84008.1"
                     /translation="MLTAWCLNELQRIKAWLVAHKPSHPLEYHSFDAVLTLWMMGWIG
                     WLPVLLFDEVLWAWPLCLLGMWAPALYVRWRARAHAAGRLRCDWLGALASTTVRV"
     gene            21146..21994
                     /locus_tag="PKN51_02550"
     CDS             21146..21994
                     /locus_tag="PKN51_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNT84009.1"
                     /translation="MNIPALTCFQLSVSDHIAHLVLSRPEAMNTMGPAFWRELDQALE
                     LLQRDGAARALVISSTGKHFSAGMALDVFGGAIQMDDAHPEGRAAIADLLADLQATFT
                     RLETLRIPVIVAIQGGCVGGAVDMVTAACIRYATQDAFFCVQEINIGMVADVGTLQRL
                     PKLVPLAVVKELAYTGRRMPAARAREVGLVNEVFDSADACLAAAMQCAREIAAKPPVA
                     IWGSKQVIHYARDHTVDDSLRQMGWVQAAIWSNAHVREAVTAMKQQRAGAFPPLATLT
                     RFTDHD"
     gene            complement(22190..23047)
                     /gene="queF"
                     /locus_tag="PKN51_02555"
     CDS             complement(22190..23047)
                     /gene="queF"
                     /locus_tag="PKN51_02555"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575038.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNT84010.1"
                     /translation="MTTPATPHDSPLGRASAYSDQYDPTLLFPIARAPQRAQLGIQGQ
                     PPFFGADWWTAYELSWLNARGKPQLAIAHIMVPCDSTHIIESKSFKLYLNSFNGTRID
                     RTEELVRRLRQDVSAAIWQGGPVQAAAGVRLIAPDDFDREAVHELDGVNLDRLDIDVD
                     GWDEPAPQCLSAAFDEQPVEEALTSRLLKSNCPVTGQPDWGSVQIRYAGPQIDQAGLL
                     RYIISFRRHNDFHEHCVERMFTDIRARCRPTKLSVYARYTRRGGLDINPWRTSHPGPL
                     PPNVRTARQ"
     gene            complement(23044..23952)
                     /locus_tag="PKN51_02560"
     CDS             complement(23044..23952)
                     /locus_tag="PKN51_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT84011.1"
                     /translation="MQIEANGIRIEVEDSGGDGPVVMLLMGLGGQLIHWPAAFVQALV
                     DAGWRVLRLDNRDAGLSSHFSAHGVPAIPWVAAQAWLGRRPRVPYTLGDMARDALGVL
                     DALGVARAHVVGLSMGAMIAQRLALLAPARLWSLTCISGCSGARGLMKPQASVLRVAA
                     GKPRAGDDAALAAYYLNFLRAIASPRYPPSDAELRDVLARTARRHAPHSDATTRQLAA
                     ILADGGRAGQLARIRCPTLVVHGADDPWVPPACAHDTARRIAGAQPALIPDMAHDLAP
                     AGHPEIVRRVLAVLLPFLPSAQVHTP"
     gene            complement(24006..24854)
                     /locus_tag="PKN51_02565"
     CDS             complement(24006..24854)
                     /locus_tag="PKN51_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNT84012.1"
                     /translation="MSQTITPELRQWIVDQARAGHSADAVLQSMKSSGWNEDVAIEAM
                     ESTLRGHLDQQAQAQGLPPAVPVPEPDVADSPLYLDGGDRQVAVLGSLALPRVVILGG
                     LLSDQECDALIAAARPRLSRSLTVATQTGGSEVNPDRTSQGMFFQRGESELVARIEAR
                     IARLIRWPVENGEGIQVLHYLPGTEYKPHYDYFDPKEPGTATIVKRGGQRVGTVVMYL
                     SEPEKGGGTSFPDVFFEVAPKRGNAVFFSYERPHPSTKTLHGGSPVIEGEKWIATKWL
                     REREFR"
     gene            complement(24863..25636)
                     /gene="yaaA"
                     /locus_tag="PKN51_02570"
     CDS             complement(24863..25636)
                     /gene="yaaA"
                     /locus_tag="PKN51_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNT84013.1"
                     /translation="MLFLLSPAKALDYDTPLPADLPHTQPLFVPEATALIEVLRQQTP
                     AQIASLMKLSDALASLNVARYQAWRPRFGPRQARQAVLAFNGDVYEGLQARTLTASQL
                     DWLQQHLCILSGLYGVLRPLDGMRPYRLEMGTALATERGRNLYQFWGPRIADHLNQRL
                     AGQPAPIVVNLASQEYFKAVDRWVLQARVIDCVFEVRQGGAFKVVGVHAKRARGLMMR
                     YAAQHRIASPQRLQGFDLEGYAFAPAASTPDRLVFRRSA"
     gene            25635..26378
                     /locus_tag="PKN51_02575"
     CDS             25635..26378
                     /locus_tag="PKN51_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT84014.1"
                     /translation="MLPILYQDAQLVAVDKPAGLLVHRTGLDAGETRFAVQWLRQQIG
                     RPVWPVHRLDKGTSGVLLLALDASCAATLAQDWAGGGVRKTYVAMVRGWPPAAGLIDH
                     PLARLPDDARGARGLERQPALTRFRTLARLALPLPDRGFAQTRCARIELQPDTGRRHQ
                     LRRHCKHMAHPIIGDATHGKGALNRAVAALLGSQRLWLHAQSLVLRHPVSGAPLHIEA
                     APGPEWALWAPWTPTWCDAPRHAASRSDI"
     gene            complement(26456..29029)
                     /locus_tag="PKN51_02580"
     CDS             complement(26456..29029)
                     /locus_tag="PKN51_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HNT84015.1"
                     /translation="MATDIEKAPEALQQLVAETDTGGRKPSGLTARLMFGLALAWAVF
                     QFWYASPLPFALGWGILNDTEARAIHLAFSLALAFLAWPAFRSSPRRHVPLVDWGLAI
                     AGAFAGAYLMLFYRELATRPGQPSVMDIVTASAGLLLLLEATRRAVGWPMAALAALFL
                     AYCVAGPWMPEVLAHKGASLSRLLSHMWLTTEGVYGIALGVSASTIFIYVLFGALLDR
                     AGGGNYMMQVSFAALGHLRGGPAKVAVVSSALNGMISGSSVSNVVSGGIFTIPMMKKA
                     GYGGVKAGAIETMSSVNGQIMPPVMGAAAFLMVEYVGIPYSDVVRHAFLPASLSYLGL
                     LYIVHLEALKLGIQPAAAAAPRPWRARLMRTLIGSSGALAVVGALYHLLQFFKSALGA
                     AAPWAVGGTLLALYLVALRQAAVSPDLPEDIDVHHPQRLETWPTVRAGLHYLIPIGVL
                     IWCLMVEEMSPALSAFWACATLVVLMLTQRPLLGLLRGVRTPGAWREGGQAVVHGFHD
                     GARNMIGIGVATATAGIIVGAITLTGLGLRMTEFVEFVSQGSVIAMLLFIAFVCLVLG
                     MGVPTTANYVLVATLMAPVVVELGAQAGLVIPLIAVHLFVFYYGIMGDITPPVGLATF
                     AAAAISGEDAIQTGIQGSLYALRTVILPFIWIFNPQLLLLDVHGWGELVLVVSACTLA
                     MLGFAALTMNWLRIRLTWWEFALLALAVVMLFRPGFFMDRLAPEHTRVPAAQVLEVAR
                     ALPDQGTLVLVIKGTTLEGEEVEKTVAVQLGAQGPDGRARLADAGLTLMTLGDAVQVG
                     AVRFGSQARKSGFEQGWDIAHVQVPTDRPSKNWFLLPGMLLMALVWGLQGRRMRRTAP
                     A"
     gene            complement(29196..30176)
                     /locus_tag="PKN51_02585"
     CDS             complement(29196..30176)
                     /locus_tag="PKN51_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNT84016.1"
                     /translation="MSLRPVLKTLAAAAALSTAALAAPASAQQKFVTIGTGGVTGVYY
                     AAGGAICRLVNKDRAKHGIRCSVESTGGSVFNVNTIKAGELDLGFAQSDVQYNATKGL
                     SQFKDGGAVSDLRAVFAVHPEPFTVVARKEAGITKFEDFKGKRFNVGNPGSGTRASME
                     ELLAAMGWKLSDFSLASELKADEHGPAVCDGKIDGFFYGVGHPSANIQDPTTSCGAKL
                     VPLTGPVVDKLVADKPYYAKVSIPAGLYPNNPQATPTYGVLATVVASSKTPADTIYAV
                     TKAVFDNFDEFKKLHPALAHLKPEDMVRNGLSAPLHDGAARYYKEKGWIK"
     gene            complement(30211..31545)
                     /locus_tag="PKN51_02590"
     CDS             complement(30211..31545)
                     /locus_tag="PKN51_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNT84017.1"
                     /translation="MSTTHVMHRHLHHTPPVAVGGQGVWLHDAAGRSYLDASGGAAVS
                     CLGHGHPDVLAAMHRQIDQLAYAHTSFFTTEVAEQLAEQLVRTAPAGMSHAYFVSGGS
                     EAVEAALKMARQYYLEIGQPERSHFIARRQSYHGNTLGALAVGGNEWRRRQFAPLLVD
                     VTHVSPCYPYRDQQPGETPEQYGQRLARELDEAICRVGPGKVLGFVAETVGGATAGVL
                     VPVPGYFKAVREVCDRHGVLLILDEVMCGMGRTGTLHACQQDGVVPDLMTVAKGLGGG
                     YQPIGAVLAQQRIVAAMSAGSGFFQHGHTYLGHAVACAAALAVQQVIERDGLLQKVQR
                     GAQVLQALLAERFAHHPHVGEVRGRGYFWGIELVAERAGKTPFDPARRLHARVRSEAF
                     ARGLLVYPMGGTVDGRYGDHILLAPPFIATDDELALIAERLAAAVDAAIAQG"
     gene            complement(31583..32356)
                     /locus_tag="PKN51_02595"
     CDS             complement(31583..32356)
                     /locus_tag="PKN51_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HNT84018.1"
                     /translation="MSLKGKTALVTGSTSGIGLGIAIELARQGAHIVMNGFGDVDAPR
                     RQIEACGVQTAYHGADMSKPAEIEAMMAFAAEKFGGVDILVNNAGIQHVSPIEGFPTE
                     RWDAIIAINLSSAFHTTRLAIPHMKARNWGRIINIASVHGLVASKDKSAYVAAKHGIV
                     GLTKVTALELATTGVTANAICPGWVLTPLVQKQIDARAAAEKISTEQATRELLGEKEP
                     SLQFTTPEQLGAMAVFFCSAAAANVRGQAWANDGGWTAQ"
     gene            complement(32538..33179)
                     /locus_tag="PKN51_02600"
     CDS             complement(32538..33179)
                     /locus_tag="PKN51_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HNT84019.1"
                     /translation="MAWTQDQMAARAAQELQDGFYVNLGIGIPTLVANHVPADKEVWL
                     QSENGMLGIGPFPTADEVDADLINAGKQTVTTIKGSSIFGSHDSFAMIRGGKINLSIL
                     GAMQVTDKGDLANWMIPGKMVKGMGGAMDLVAGVARVIVLMEHVAKKKDGSTDMKILP
                     QCTLPLTGVGVVDRIITDLGVFDVTDRGLKVVELAPDVSFDEVQGKTGVPLLQ"
     gene            complement(33196..33897)
                     /locus_tag="PKN51_02605"
     CDS             complement(33196..33897)
                     /locus_tag="PKN51_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNT84020.1"
                     /translation="MNKIYPSAADALKGVVHDGQQLAVGGFGLCGIPEALIDALRDSG
                     VKNLTVISNNAGVDGFGLGKLLETRQIKKMISSYVGENKEFERQFLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGVGTLVAEGKELREFDGQTYVMERSLVADVALVKADVA
                     DRSGNLRFNLTARNFNPAAAMSGKLCIVEVERIVANGELAPDDIHLPGIYVHRIVHNP
                     TPEKRIEKLTLREHK"
     gene            33987..34583
                     /locus_tag="PKN51_02610"
     CDS             33987..34583
                     /locus_tag="PKN51_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNT84021.1"
                     /translation="MAIPVPAAGDPALAPPDYRLAFQLAPVGLILSRQRHIVDCNDRV
                     CDIFGAEPAQLIGQSFRILYPSADEYERFGARIAPILNRDGLYADNRVMQRVGGPQAG
                     EAFWCHVTGRAIDRRAPHAAGIWSFEDLSAQRPMGAVLTAREREVAAQLTCGLTSKEI
                     GRALGLSHRTVEVHRAKLMRKYRAGTTAELVQKLMGGL"
     gene            complement(34654..35055)
                     /gene="pcaC"
                     /locus_tag="PKN51_02615"
     CDS             complement(34654..35055)
                     /gene="pcaC"
                     /locus_tag="PKN51_02615"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HNT84022.1"
                     /translation="MTLPDDPAFQHGLKKRRQVMGDDFVDRAFASATPFTEPMQHAIT
                     RNAWGDVWQRPGLDLKTRSLVTVAMLTALGRQHELKGHVRGALRNGATVAELQEVLLH
                     ASIYCGVPCAVEAFRSAAEVVDGGPGQSDKK"
     gene            35125..>35705
                     /locus_tag="PKN51_02620"
     CDS             35125..>35705
                     /locus_tag="PKN51_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796207.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HNT84023.1"
                     /translation="MSRYPLPSLDSLPEDIRARILEVQAKAGFVPNVFLALARRPAEW
                     RAFFAYHDALMEPESAGRSSRLSKGDREMIVTATSAANGCLYCVVAHGALLRIYEKKP
                     LVADQVATNHRKADISARQRAMLDFAMKVSQHPRELGEEDFAPLHAHGFDDEDIWDIA
                     AIAAFFGLSNRMASFSNMTPNAEFYLMGRVPRE"
BASE COUNT         5336 a        12680 c        12438 g         5251 t
ORIGIN      
        1 cgaagtggtg ttctacgacg gcaaggccat ctcggtcgag ctgcccacca gcgtcgagcg
       61 cgaaatcacc tggaccgagc cggccgtcaa gggcgacacc tcgggcaagg tgctcaagcc
      121 ggccaagatc gccaccggct tcgaggtcgc ggtgccgctg ttcgtcaacc agggcgacag
      181 gatcgagatc gacacccgca ccggcgaata ccgccgccgc gtctgacgca ggccagcgtg
      241 cacccccaaa aaaagccccg cccggggctt ttttgttgtc gacgggaggc gatgcaaggc
      301 gcaaggcgca gcaacagccc gtgctgtggc gagcattggc aacgccacag cgtgcccgta
      361 tcgggcggac agcgccgatc cgatttcagt gttttcagca ccaggttcag tggaccaggt
      421 tcaaacttca cgataccgaa gccatacgat ggcaggcggt caacggctgc cgacaggttc
      481 aataagatcg cgcccatgga aaccacgcaa gacatcaaga cgtcgtcgct cacctcgatc
      541 caggacgcct gggccacgtt gcaggcacat gcgcagaacg gcaccccgct cgaaccggcg
      601 gcctaccgcc tggcctttgc cgaccccgaa ttcctgctgc gccccgaaac ccggggtgtg
      661 cgctttcagc tcgagctgct caagcccgac ctcgacctga gcgccgccgg cgtgcacagc
      721 accgtggtgg tctatggcag cgcacgcttc gtcgcgcccg acgaagcggc gcagcagctc
      781 gccagcgccc gcaccagcgg cgacgccgcc gcccttgccg cggccgagct ggcggtgcgc
      841 aacgccgtct actacgccca ggcgcgtgag ttcgcacggc tggtggccac ccacagcgag
      901 cgccagcgcg ccgaggaccg gttgttcatc tgcaccggcg gcggcccggg catcatggaa
      961 gcggccaacc gcggcgccca cgacgtcggc atgccctcgg tcggtctgaa cattgcgctg
     1021 ccgcacgaac agcacaccaa cgcctacgtc acgccctacc tggcgttcaa gttccactac
     1081 ttcgcgctgc gcaagatgca cttcatgatg cgcgccaagg cgctggtggt ctttccgggc
     1141 ggtttcggca ccctggacga gctgttcgag gtgctgaccc tggtgcagac gcgcaaagcc
     1201 aggccggtgc cgatcgtgct gtgcggcagc agctactgga gccgcctgct caacctggac
     1261 atgctggtgg aagaaggcgc catcgcgccc gaggacctgc agctgtaccg cgtggtggac
     1321 acacccgccc aggcctggga cgccatccag agcttctacc gcctgtgacg cgggtgaccc
     1381 tgcggcgggc tcactggcgc agcacgtcgg cgccggccgg gggcttgaac tcgaaggtgc
     1441 tggccggcaa ggccgggttg acctcgatcc gcccgaattt cagcaccgag cgctggccaa
     1501 ggctgtcgac gatctccagg gcggccagcc gctcgccgtc aaagccgacc agcacctgct
     1561 gcagctgacc gtcgcgctgc ttcggcgtgg cgctgaccca ttgcagaccg tcgcggtccg
     1621 gcgccgccgc cagcgtgaac tcggcctgca gcgcacgcag atcgggcgcg gcggcaatca
     1681 gcgccgccgg cgtctgcccc agcacctgcg cttgcgcgcg ctgcgtcacc tggttgaggt
     1741 cggcgtcata cagccacagc gtcttgccgt cggcgacgat ggtctgcacg aacggcttct
     1801 ggtagtcgaa ccggaaccgg ctcggacgct ggaattcgaa gcgaccgctg gacgtcttga
     1861 cccgcgccgg ctgacccggc ttggccggtg ccgtcacggt ctgcgtgaaa tccgcgctgc
     1921 cgctgcggct gtggcgaatg aaggcgtcca ggttctgcaa cccgtcggcg cgggccaccg
     1981 gcgccccgag ggcgaaccac accaaggcga ccgcggacag cctaggaaca tgcatggggc
     2041 aaggtttcat ggtgactctg agcgacgcag cgcggcgaaa gttcggtcgg tgacgcctgg
     2101 atacggcggc acaccatggg gacacccggc cgcccccagg ctgcgccaga acgaggagga
     2161 acccgacccc acgttgcggc gcgccacgga tgggcatggg atgcacgacg ccaggcgcag
     2221 acagtgccga agccatggcg cccctgagcg gcgccgcaga ccaccccggg caggcatgcg
     2281 acgcgcgcga agcacctgcg accaggaggc tgtcggactt ggagcgccga cagcgcgcat
     2341 cggccccagc gaggcccgtt gttgccggat aggcagccca acagccgagc cattggggaa
     2401 aaaggcggcg ctctcgaaac gcggcgaagc caagaaaggg gcgctgcggt cgatctgcag
     2461 ccggcgattt ccaggtccga cagtctgcta gacatcgggc cgctcgctgg caccggcgtc
     2521 ggccggggaa ggcggcggct tgcgcggtgg cttggggcgt ggtggcctgg aggtgtggat
     2581 ggcctgcgtg gccgccgtca tgtcgccggc cgcgagcagc gcaaaggcgc gcgcggcgcg
     2641 atcgatggcc tgttcgatgc cgatctgttc gtccagcggc ggcttcttca gcacccagcc
     2701 gaccacctcc tgccggtttc ccggatggcc aatgcccagc cgcaggcgcc aatagtcgct
     2761 gctgcccagc tgcgcatgga tgtcgcgcag accgttgtga ccggcgtggc cgccgccctg
     2821 cttgagtttg gcctcgccgg ccggcagatc cagctcgtcg tgcgccacca ggatttcctg
     2881 cggcgccacc ttgtagaagc gcgccagcgc cgccaccgac ttgcccgaca ggttcatgaa
     2941 ggtctgcggc tgcagcagcc acacgctggc gccctgcacg ctggcgcgcg ccaccagacc
     3001 gtgaaaggcg cgctcgggca ccagccgcac gcccagctgc cgcgccacct ggtcgatcca
     3061 ccagaagccg gcgttgtggc gcgtggcagc gtattccggg ccgggattgc ccaggccgac
     3121 gaacagcttg atcatgggtt gcgattatcg gcgtgaccac agccgggccc gcaggaccgc
     3181 ccatgaaaac ggcccgctgt ccgcgggccg tcggtggtcg tcacggcagg ctgagcccgc
     3241 gcgcaacgat cacttcttgt cgccttcggc ggccgccggg gcttcgcctt cagccgcctc
     3301 ggccgccggg gtcaccaccg acaccagcac cgtatcttcg gcgctgtggc cacgcagcac
     3361 cggctcaacg cccttgggca gcgcgatgtc cttgagcgtg acggtcgcac ccttttccag
     3421 cttggacaga tcgacctcga tggcttccgg caggtcggcc ggcaggcagc tgatttcgag
     3481 ctcgttcagg atgtggttga tcaggcactt gtccagcttg acggcgttgg actcttcctc
     3541 gccacggtag tgcagcggca ccttcatgtg gatcttctgc ttggccgaca cgcgctggaa
     3601 gtcgacgtgc agcaccagct gcttgtaggg atggacctgc acatcgcgca gcagcacctt
     3661 ggcggtcttg ccgcccagct ccatgtccag caccgacgag tggaaggctt ccttcttgat
     3721 ggcgtgccac agcgcgttgt ggtccagctc gatcagctgg ggctcaccct caccgccgta
     3781 gacaatgccc ggcaccttgc cggcattgcg cagacggcgg ctcgcacccg ttccctgctt
     3841 gctgcgctcg aaagcgacga atttcatgac gtactccttg tgggccgacc gcgaccagtg
     3901 cgacccgggt tgaaaacggt tcaccgccac cacggtggcc gcgcgaacct cgaatggttc
     3961 ctcagaacag gttttcctgc tcctggaaca gacgcatcac cgactctccg cgggcgatgc
     4021 gctggatcgt ctgggcgatc agcggcgcca cggaaagctg gcgaatcttg gggcatgccg
     4081 ccgccgcggc actgagtgga atggtgttgg tcaccaccac ttcgtcgagc gcgctgccct
     4141 tggcgatgcg ctcgatggcg gggccggaga agatcgggtg cgtgcagtag gcgtagacct
     4201 ttttggcgcc gcgctgcttg agcacttcgg ccgccttcac cagggtgccg gcggtgtcga
     4261 tcatgtcgtc catgacgacg cagttgcgcc cctcgatgtc gccgatgacg tgcatcacct
     4321 cgctctcgtt ggccttgggg cggcgcttgt cgatgatggc caggtcgcag ttgagctgtt
     4381 tggccagcgc gcgcgcgcgc accacgccgc cgacgtcggg gctgaccacc agcaggtctt
     4441 cgtagttctg ctggcgcaga tcgcccagca gcaccggcga ggcgtagatg ttgtcgaccg
     4501 gaatgtcaaa gaagccctgg atctggtcgg cgtgcaggtc catggtcagc acgctgttga
     4561 cgccgacggc ctgcagcatg ttggccacca ccttggccga gatcggcacg cgggtggaac
     4621 gcggccggcg gtcctggcgc gcatagccga aatagggaat cacggcgctg atgcgttcgg
     4681 ccgaggcgcg cttgagcgcg tcgaccatga tcagcagctc catcaggttg tcgttggtcg
     4741 gcgcgcaggt ggactgcacc acgaacacgt cgcgcgcacg cacgttctgc ttgatctcga
     4801 ccgtgacctc gccgtcggag aagcgcccca catcggccgc gcccagactg atgccgaggc
     4861 tgctggcgat ctcggcggcc agcgtcgggt tggcgttgcc ggtgaacacc aggaaatcgg
     4921 aagggtcaga gggctgcatg cgctttccgg cggaatgaca ctgaaacggt acggcaagaa
     4981 gccgaaatgg cgtgcctgct ggcacgccac ccggtcacag tcatttggca ggggaggaag
     5041 gattcgaacc ctcgcatgcc ggaatcaaaa tccggtgcct taaccgactt ggctactccc
     5101 ctacacagga aaccgacccc ataccaccgg gccaaccccc ttcaaacttc acctggcgcc
     5161 caaccgccaa gcgggtggaa ctccagattg ctgcaccagc gcaagcccca gtcgggcgga
     5221 acctgcacct cggtcacgcc ggtcatggcg cctggcacca ccggagcgaa caccgcactg
     5281 cccgagccgg tcattctgcc atgcaggcca tgctgcgcaa gcagcgccag cccctggcca
     5341 acctgcggac aaagccgctc ggcaaccggc tgcaggtcgt tgtggccaaa gccatacggg
     5401 tctgcagcaa agcctgcgat tatagcaggt ttgctgtctc gcttcagggc cgggtcgcga
     5461 aaaatcgctg ccgtttccag cccctgcggc ggcttcacca caacaaaacg cgccggcggc
     5521 agttgcagcg tgcggatgcg ctcgccgatg ccctccaccc agccatgtgt gccatgcagg
     5581 aagaacggca cgtcggcgcc cagcgccaga ccgatgcgcg ccagctgcgc ccggttcagg
     5641 ccaaggcgcc acaggcggtt gagcgccagc agcgtggtgg cggcgtcgga cgagccgccg
     5701 cccagtccgg cctgtgccgg aatgcgcttg tgcaccatga tgtgcgcgcc ctcggcgcag
     5761 ccgctggcct gctgcaaggc gcgcgcggcg cgcaggatca gatcgtcggc cggcagggtc
     5821 ggccccaggt cctggcggct gagggcgccg cccgcgcgcc gctcgacgtg cagggtgtcg
     5881 caccagtcga tcagcatgaa ggccgattgc agcaggtgat agccgtccgc gcgccggccg
     5941 gtgacgtgca ggaacaggtt cagcttggcc ggcgccggca ggtcgtagaa cgcgcgcatc
     6001 ccggttccgc gcctcagcgg tccagcacca gccgcaattc ggccgtgggc aggggcatca
     6061 ggcgctgggc gaacagccgc ccgtcgggca gaccgctcag gtcggcctgc cagccgggca
     6121 ccgactgcgg ctgaccgtgc agccagccaa acagcgcgcg caccggcagc ggcgtgccgg
     6181 tgacctgctc caccagcgcg tcgagcgatt cgaagcgggc ggtctgctcg ccctgctgca
     6241 gcagcgcctg accgggctgc cagcgcatca cggccagggc gctgcccagg ggcgaggtga
     6301 gcatcagctc gcccaccagg gcgttgccgc tgagcgcaaa accggccgag tacgactgcg
     6361 gcggctcgga cgccacggtc agccccaggc gcccgctcca atggctggtt tgcgagtcaa
     6421 atggggcgtt ttggcccgtc tggtgtgcgc aaccagctat cattaaaata gcacacaggg
     6481 cgccgccgag gcgccggacg gcggccttca cagccgcacc cgcagacgca gcagggtttt
     6541 gcgcagcgtc tcgttgtcgg cctccaggcg ctggccttcg cgccacacgc gcagcgcggc
     6601 ctcgcgctcg cccaggatcc acagcacctc gcccaggtgg gcggcgatct cggcgtcggg
     6661 gcgcttgcgg aaggcggctt cgagggtctg gcgtgcctgg cgcaggttgc cctgtcgaaa
     6721 ctgcacccag cccaggctgt cctggatgta ggcgtcgttg ggcgacagct gcaccgcttt
     6781 ctcgatcagg gttctggcct cgggcaggcg caggttgcga tcggccagcg catagccgag
     6841 cgcgttgtag gcgtgcgagg cgccgggctt gagttcgatc aggcggcgca gcaggcgttc
     6901 catcacctcg aaccttccca cgcgctcggc cgccatggcg gcttcgtaga gcagggtttc
     6961 ctcgtcgggg gtctgttgca gcgcgccgtt gagcagttcg taggcctgcc cgggctggcc
     7021 gtgatcgcgc agcaactggg cttcggccag cagcttgagc cgggcatcct cggggctgcg
     7081 ctcgggcgcc gcacggatgg actggcgcgc ctcctccagc cgaccctggc gcgccaggat
     7141 ctgcgccagg cgcgtctgca cggccagtgc ctgctcgggc gaatcgaccg aggccagcca
     7201 ctgctgcgcc tgcgtcagct cgccgcgccg ctcggcgatg cccgccagca tcatgcgggc
     7261 gtgggcgctg ccttcgggcg gctcggcgtc cggcgtcgcc ggtgtggcct ccagcaggct
     7321 caggtagcgg cgcaggtcgg cttcggcctc ggcgtccttg cgctcgtcgg catacagcgc
     7381 cccgcgcacc agccaggcct ccgggtgctc cggcgcccgc gcgatcaggc ggtccagctg
     7441 gcgatgggcg tcgcctccgc ggccctgctc gatcagcgca cgcgcgtagg cgatgaacag
     7501 ctcgggccgc gcctgcgcag tggacagatg gcgctcgacc agggcttcgg cctgggtttc
     7561 gccgctgccc atcagttgca gcccgagcag ggccggccac tccgaagatg cgtcggcctt
     7621 ctggcccagg gttgcggccg ccaacgcacc ggccttgtcg ccggcctgca ggcgcagccg
     7681 gccgatgctg gtccaggccg ccggcgccag cgccggctgg ctgagcgcgc cgtccagcgc
     7741 gcgctcgatc acgcgtgcgg cctcggcctt gtcgggcacg cgctggtaga gcgcgggcag
     7801 cgccgtgatg aagctcaggc gctcggcctc cggcagggtg gccaggaaat gccgcgtcgg
     7861 ctcctcggtc tcggccaccc gaccgagcac gatcagcacc tgcagctcgt actggccggc
     7921 ctgggccgaa tccgggaacg cctggcgcca ggcccgcgtg gcttccagtg ccgcagggcc
     7981 ggcgcgcgac tggatagcca gctcggtggc gcgcttgaac aaggcctcgt cgcccgtgcg
     8041 ccgggccgcg ttcagcatgt aggcggcgcc ctgctgggcc tcgccctcgc gcgccagcag
     8101 ctcggccagc agcagctcgt acagcagcct ggcgttcaga cgcgaggtct cgggcacgcc
     8161 cgcctgggca gcgccggcct gtggtgcagc aggagcctgg gcggcggccg cccatgccgc
     8221 acacgccagc accgccccgc caagtagccg ttgaaaccgc atcatcgatc cataataaat
     8281 gatgcccagc gatgccactg cccgccccga acgccatgcc ggagctgcct gaagtcgaag
     8341 tcacgcggct gagcttcgcc gaccgcatcc gcgacgcgcg catcctggcc gcgcgccccg
     8401 gtctgccgct gcgctggccg ctgggctgtg ccccggagca gctggtggac cgcacggtgc
     8461 aggacgtgcg gcggcgcggc aagtacctgc tgctgcacct cgatcagggc gtgctgctgc
     8521 tgcacctggg catgtcgggc agcctgagct ttgacgccgc accggcggcg ccggggcgac
     8581 acgatcattt cgacctggtg accacgcacg gcacgctgcg cctgcacgac ccacgccgct
     8641 tcggcgccgt ggtgtggtcg gccagcctgc agctcgaccc ggcgcgcaag ttgctggcgt
     8701 cgctgggcat ggagccgctg ggcgagcggt tcgatccggc ggccttccat gccggactgc
     8761 gcggccgccg tgcgccgatc aagcaggtgc tgctggccgg cgacgtggtg gtcggcgtgg
     8821 gcaacatcta cgcctccgag gcgctgtttc aggccggcat ccggcccacc gtgcgcgccg
     8881 accggctgtc gcgcgcccgc gccgaccgcc tgcatgcggc ggtgcggcag gtgctggcgc
     8941 gggcggtcga gcgcggcggc agcaccttgc gcgacttttc cagcgccacc ggcgagagcg
     9001 ggcactttca gctcgaagcc gcggtctatg ggcgcgccgg gcaaccctgt ctgcaatgcg
     9061 ccacaccggt gcggctgatc cgccagggcc agcgttccac ctacttctgc ccccgctgcc
     9121 agcgcgcctg acgcgaactt gctcacgacc cgcggacccg gtcgcgcgtc gtgaccatgc
     9181 caagccgggt tacaccatgt aatatggtcc caacgatccg gagtggcatg gtggcggctt
     9241 tcagcgaaca gttcgacagg cacggggcat ggcggcgcga aatgagttcg cgcctgaaga
     9301 acctggcggc ctggctgcgg cagcagcgcc tgctggacga cgcgaccgac gaaagcctgc
     9361 agcgcctggt cgagcagttg cgctccgaca aggtgatggt ggccttcgtg gccgagttct
     9421 cgcgtggcaa gtcggagctg atcaatgcgg tgttctttgc cggctacggt cgccgcatca
     9481 tgccggccag cgccgggcgc accaccatgt gcccgaccga gctggcctac gacgccgagc
     9541 tgccgccctg cctgcgcctg ctgccgatcg aaacgcgcct gcagccccag gccctgatgg
     9601 aatggcgcat ggcgccggag cagtggacgc gggtcgatct ggacgtgaac gacccgcagc
     9661 agctggccag tgccatggag aaggtatccg aggtgcgtta cgtcgaccag gcgcaggcgc
     9721 gtgcgctcgg cttctggcac gacgaccgcc cggaagacaa cccgccggtc aacgcccagg
     9781 gcctggtgga ggtgccgcgc tggcgccatg ccctcatcaa catggcgcac ccgctgctca
     9841 ggcagggcct ggtggtgctg gacaccccgg ggctgaacgc catcggcgcc gaacccgagc
     9901 tgacggtgaa cctgatcccg caggcgcagg ccgtggtgtt catcctggcg gccgacaccg
     9961 gagtgaccaa gtccgacctc ggcatctggc gcgagcatgt ggccaccatg caggacagca
    10021 gcagttcgcg cttcgtggtg ctgaacaaga tcgacgccct gtgggacgag ctcagcacgc
    10081 cggcgcagat cgaagcgcag atcgcccgcc aatgcgccga caccgcgcgc accctggcgc
    10141 tgcaggacga tcaggtgctg gcgctgtcgg cgcagaaggg cctgctggcc aaggtgcgcg
    10201 gcgatgcggc cctgctggcc gacagccgcc tgccgcactt cgaggatctg ctgggccagc
    10261 aggtgctggg cgcgcgccac gccatgctgc acgacgccat cggtgccggc gtggcccgtg
    10321 cccaggccga ggcggaacgc accctggcca tgcgcgggcg cgagctgtcc gaccagacca
    10381 tcgagctcaa cggcctgcgc ggcaagaacc accaggtgat ccaccagatg cgcctgcgca
    10441 tcgagcagga gaaggccgac ttcgaccgca gcgccgccag cgtgctggcc gtgcgttcgg
    10501 tgcacctgaa gctgctgcgc gaggtgttcc agctgctggg caatgcgtcg atcaagcgcg
    10561 agctggccga gctgaacgcc gccctgcgcg agccgggcct gaagctcggc atcaagaagt
    10621 cctacgccga cggctttgag cggctgcgcg gcgtgatccg gcaggtgcag gcgctggaag
    10681 gcgagatcca cgccatgctg aaggcggcgt ttgtccagct caacaccgac tacgggtttg
    10741 cgctgcaggc cgccgagccg cccgccatgg agcgctttgt cagcgacctg gacgcggtcg
    10801 agcgcagcca cctgcgctac ctgggcgtgg gtcacatgct ccagctggcg cgcgccggct
    10861 tctccgagaa gctggtgcgt gcgctgctga cgcgggtgcg ctcgatcttc gaaaccgccc
    10921 tgggcgacat cgagctgtgg aacaaggcca ccaccagcca gctcgacacc cagctgcgcg
    10981 agcggcgacg cgcctacggt cgccgcatcg acgcggtcga acgcatccag gacgcggccg
    11041 gcagcctgga ccagcgcatc cacgacctcc agcaggagca gcaggccctg caggccacgg
    11101 cccagcgcct gcgcgagctg gggcacgagc tgcgccgcgc cagcgccacc gacgctgccg
    11161 acaccgcccc gccggagctg accgaggtcg tcccggcgtg aacggcggcg gcgccgagct
    11221 ggccgcgcgc gtcatggcct ggcagcgccg ccaaggccgc cacgacctgc cctggcaagg
    11281 cacgcgcgat ccctatcgcg tgtggctgtc ggaaatcatg ctgcagcaga cccaggtgac
    11341 cacggtgctg gcctactacc cgcgctttct ggcgcgcttt cccgacgtcg aacggctggc
    11401 cgcggccagc gaggacgagg tgctgggcct gtggagcggc ctgggctact accgccgtgc
    11461 gcgccacctg caccgctgcg cgcgcgacgt ggtggcgcac catggcgggc gctttccggc
    11521 cagcgcggcc gagctggcgc ggctgcccgg catcggccgc tcgacggcgg cggcgatcgc
    11581 ggcgttctgc ttcggcgagc gcgcggccat cctggacggc aacgtcaagc gcgtgctgac
    11641 gcgcgccctg ggctttggcc acgacctggc gctggcgcgc cacgagcagg ccctgtgggt
    11701 gcaggccgag gccctgctgc cgcgcaccga cctgcggcgc gccatgccgg tctacaccca
    11761 gggcctgatg gacctgggtg ccagcgtgtg ccaccggcgc cgccccgact gcccgcgctg
    11821 cccgctgcac gacctctgcg tggcggccgc cgcaggccag cccgagcgct acccggtcaa
    11881 gcgccggcgc gtggcgcgcc agcggctcga gctgtggctg ctgctggcgc gcgacgcgca
    11941 gggccgcgtg tggttgcagc agcggcccga gcacggcatc tgggcgcgcc tgttctgcct
    12001 gccgctgttc gacgatcggg cgaccctgct ggcgagcctg ccggcgcggg tgcacgggca
    12061 ggccgtcacc gagccgccgc gccgccatgt gctgacgcac cgcgacctgg acctgcaccc
    12121 ggtcagcgtg cggctgcgcg ccaccgatct gcgccacgcc gacgggcgct ggttcggcgc
    12181 cgacgaatgg ccggcgctgg ggctgccagc gccagtgcgc cgcctgctgg cggcaccggc
    12241 cgcggcgccc caggcgcccc gcggcaggct gggcgaagtg cgtccttcag ttcaaaaata
    12301 atagctgctg gcgcttgctg gtcgccggct caggccatgt ttctcatgaa tcggtggccg
    12361 ccggcgacag cacatcggcc accgtcgcct cgctgcgcag gccacgcgcc caccagccgg
    12421 ccagcgcctc gtcgggcgcc agcagcaggc gctccaccag cgggcgcagc agctggcggc
    12481 gcggctcgat cagcagcaca tggcgcggct cctcgttttc gctgcgggtg cccagctggc
    12541 cgacccagtc cagcccggtc agcgccgcca tggccgcaga cagctgcaga gcgtccaccc
    12601 gcagcgcctg cgccagctgc ggcagcgtca gaccgcgccc ggcgccatcg cgcgcggcgg
    12661 ccagcgcctg cagcagctcg agcgccagcc ggaagtccca gcccggcccg ccgccgcgcc
    12721 gcgccacgcc cgacagcagg ctgggcaggt aggcggcgat gacggccccg aacagcacga
    12781 tcagccaccc caggtagatc cacagcagca ggatcggcag cgtggccagg gtaccgtaga
    12841 tgaccgaata ggtgggcatg cgcgtcaggt accaccccag tccctgccgc gccagcccca
    12901 ggcagacgct gacgaacagc gcgccggcca gcgcgtgacg ccagcgcacc ggcgtgttcg
    12961 gcaccaggcg gtacagcagc gtcaggctgc ccaccatcag cagcagctgc agcagcgcca
    13021 gcgccgcgtc cacgctgccc ggcaggtcgg gcagccagcc gcgcgaggcc gtcaccacgt
    13081 aggagctgat ggccaggctg gcgcccatca ccagcggccc cagcgtcagc gccgtccagt
    13141 acaccaggat gcgctggctc agcgggcgcg tctggctgct gcgccagatc tggttgagcg
    13201 tcttgtcgat ggtcagcatg agcgacagcg acaccagggt cagcccggca aagcccagca
    13261 gcccgagctc gctggcctgg ccggcgaact gggtcaggta gcccatcacg ctgtcggcga
    13321 tgctggccgg gatcaggctg tcgatcaacc actgctgcag cgcctgctgc aactgcccga
    13381 aggccggaaa ggcggtgaac accgacagcg ccacggccac gaagggcacc agcgccagca
    13441 ccgtggtgaa ggtcagcgcg ctggccgtca ggcccagccg gtcctgccgg aagcgctgcc
    13501 acagcgtgtg cgcggtgggc cgcaccggaa agtcggtgag ctcggtcaga aaggcctggg
    13561 cccgcgccgc cagcgcatcg aactgcatgc tcgttatgat gccaccgccc atgcccgccc
    13621 acccctcgcc tgccgtgcag gccacccgcg tcgtcgccgt cgccagcctg ctggcgctga
    13681 tggcgctcgg cctggcgtgg gagctgtggc tggcgccgct gcgcccgggc ggctcgtggc
    13741 tggccctgaa ggtgctgccg ctggcgctgg cgctgcccgg gctgctgcgc tatcgcatgc
    13801 agacctgtcg cggcctggcg ctgttcgtct ggctgtactt cgtcgaaggc gtggtgcgcg
    13861 ccaccagcga ccacggtgtg tcggcgcgct gcgccctggc gcaggtggcg ctggtgctgc
    13921 tgctgttcgc cgcctgtgcg gcgcaggtgc ggctgcgcca gcgagcggcg cgcgcggccg
    13981 ccgccggccc cgcggtttcg cattgatttc ggcatccagc gcttgcccgg caagcgctgg
    14041 cagctatcaa ttcaggagtt attgacatga ccaccgacac ccccttgctg gatctgctgc
    14101 gccagacggt gggcgcaccc catgtgctga ccgagggcga cctgacggcg tacgagcagg
    14161 actggcgccg ccgcgtgcgc ggccgcgcgc tggcggtggt gcgcccgggc agcacggccg
    14221 aggtggcagc ggtggtgcgc gcctgcgccg cgcacggcgt ggccatcgtg ccgcagagcg
    14281 gcaacaccgg cctgtcggtc ggctcggtgc cggacgacag cggcacccag ctgctgctga
    14341 acctgggccg cctggacagg gtgcgcgcca tcgaccgcgc caacctgacg ttgacggccg
    14401 aggccggctg cgtgctgcag cggctgcagg ctgcggcgca agacgccggc ctgctgtacc
    14461 cgctcagcct gggctcggaa ggcagctgca tgctgggcgg caacctgggc gccaacgccg
    14521 gcggcacgca ggtgctgcgt tacggcaaca cgcgcgagct gtgcctgggg ctggaggtgg
    14581 tgaccgcgca gggcgaggtc tgggacggcc tgtcgggcct gcgcaaggac aacaccggct
    14641 acgacctgcg cgacctgttc atcggcagcg aaggcacgct gggcatcatc accgccgcca
    14701 ccatgaagct ctacccgcgc cccgccgcca cgctgaccgc ctgggccgct gtgccctcgc
    14761 tggcggcggc gctggagctg ctggggctgg cgcagcgcca cctgggcgcg gggctgaccg
    14821 gcttcgaggt gatgggccaa ttcgcgctcg gtctggtgga tcgacacctg ccgcagctgc
    14881 gcgtgccgct gtggcgcgac acgccctggt gcgtgttgct ggaaaacgcc gaccaggaaa
    14941 gccaggccca tgcgcgtgcg cgcttcgagc cgctgctgga agccgcgctg gacgccggct
    15001 gcgtcagcga cgccgtggtg gccgaaagca tcgcccaggc ccgtgcgctg tggcacatcc
    15061 gcgagagcat tccgctggcg caggccgaag aaggcctgaa catcaagcac gacatcgccg
    15121 tgccggtgtc gcgcgtgccc gacttcgtgg ccgagaccga agccctgctg gcgcgccaga
    15181 tcccgggcgt gcggctggtc gtgttcggcc acctgggcga cggcaacctg cacttcaacg
    15241 tgcaggcgcc cgaaggcggc gacgcggccc agttcctgcg ccagcaggaa gagcgcgtca
    15301 acctcctggt gttcgacgcc gtggcgcggc accagggctc gatcagcgcc gagcacggca
    15361 tcggcagcct gaaggcggcg ctgctgccgc gctacaagtc gccggtggcg ctgacgctga
    15421 tgcgcgccat caagcaggcg ctggacccgc agggcctgat gaacccgggg cgcgtgctgg
    15481 cgcgcgaccg ttgatcgggc ctgtggatcc aggctcggcg gcgtcgtcca gcctgcgcgt
    15541 catgtacagc gcctggctgc cgtagctggc cagcgccggc ggctggcgga gcgccaccac
    15601 gaagccctgg cgctgccaga actcgcgtgt gccctgcacc gacaccagcg ccgcctgacg
    15661 cacgccacgc gcgcgcgcct gacgccaggc cgccgccagc aggcgctggc ccaggccctg
    15721 gccggcgtgc tcgggacgca ccgccagatc gtgcaggtag agcgtgtcgg ccgcggcctg
    15781 cggcgcgaag tcgccgtgca gcggcgtgac cgcgccccac accgaccagt gcgccgccag
    15841 gtaggccgcc agcgcgccgc cggcgtgcag gcccaggccg cagtgcgccg ggctggccag
    15901 gcgccgggcg tacacggcgg cgtcttccag cagcgccggg ccgtagcagg cggcctgcac
    15961 ctgcagcagc gccggcacgt cggcgccggc cagcggcgca atgcgccagc gcgacgcgtc
    16021 ggcgcccatg gctcagcgac ccgcgcgccc cggggtgtag gggcgcggcg cgccaccggc
    16081 gcgggcccgg ccggcggccg cacgcgggcg cgccgacgcg cccgggctgc tcttgttttg
    16141 atagctgccg gcgcccgcca gacgggcggc agggctgaat tcggcctgac gatcgcgggt
    16201 cggccgggca gccccgcgcg gcccgccagc gcggcgcgcc ccgccatcgt gcgcggccgg
    16261 ccggcgcggc gcgcgcggct cgctgacggg cgggaaatgc cgcgtcggct ccagcccggc
    16321 gaccaccagc ggacggaacg gctggcgcgt gtaggcctcg atctcggcca gccggcggcg
    16381 gtcgcggaac tcggccagcg tgaccgccag gccgtcgcgc ccggcgcggc cggtgcggcc
    16441 gatgcggtgc gtgtagtcct cggccttcat cggcaggcca tggttgatga cgtgggtgat
    16501 gctgggcacg tcgatgccgc gcgccgccac gtcggtggcc accaggatct gcaccttgcc
    16561 ctcgcgcagc gcgcgcaggc gccggttgcg cagcgcctgg ctgagcgcgc cgtgcagcgc
    16621 cacggcggca aagccgcttt gctgcaggtc ggcggccagc tggtcgcact cgatctgggt
    16681 gcaggcgaac acgatggcct ggtcgatcga cgggtcgcgc agccagtagt ccagcaggcg
    16741 gcgacggtgc tcggggctgt cggcccagta cagctcctgg cggatgttgg cgtggctctg
    16801 ctgcggcgtg gccacctgca ccttgcgcac gcgggcgccg ccgtcgtgca tgacgcgcag
    16861 cgccagctgc tgaatcggcg ccgcgaaggt ggcgctgaac atcatggtct gctggcgctg
    16921 ggcggtgagc tgatgaatct cggccagggc gtcggcaaag ccgaggtcga gcatgcggtc
    16981 ggcctcgtcc accaccagaa agcgcacctg atccagcacg atctggccgc tgcgctgcaa
    17041 gtccagcagg cgacccggcg tggccaccac cagggcggcg ttctgcagct gggcgatctg
    17101 ctggccgtag ggcatgccgc ccaccacgtt ggcgatgcgc aggccacggc agtggcgcac
    17161 caggtcgatg gcgtcgcgcg ccacctgctg cgccagctcg cgcgtcggac acagcaccag
    17221 cgcacccggc acggcgggct tgaactggcg cgggttgagc gggttcttgc ggcgcggctt
    17281 gcggggcgcc ggctcgccgc gcgccagggc ttcggccacg cgggcgtccc aggcggcctg
    17341 ctcgcgcgcc tgggcctcgc gctgcatgtc cagcagggtg tgcagcaccg gcagcaggaa
    17401 ggccgccgtc ttgccgctgc cggtctggct gctgaccatc aggtcgttgg cgccctcggg
    17461 gtgcgcctgc agcgccagcg gaatggcctg ccgctgcacg tcggtgggcg cgctgtagcc
    17521 cagctcggcc accgccgcca gcagctcggg cgccaggccg agggcctgga agggattgtc
    17581 ggatgctgcg ttttcagtag cttgcggcgc ttgactgtcc agcgtcagga cgtggttttc
    17641 ctcgaacgag gccatgggac atgcactttc tcgcacgcca cggcgccctg cgcgcccggc
    17701 gtgtcggatg gtggcgcgcc tgggcgcgcc gggttcatga atcgacaccc accatccgac
    17761 agcgcgaagt cccagcgccc acggtggcgg cggctggtct tcggaaacaa cgacctggcc
    17821 ccgatgaggg cccaaagtgg ctggaatgct ggccgccgaa aaaccggtgg cggccgcagg
    17881 caggaaggaa gatgcggtct gctctgatga atcgcgtggg cctgtgccct gccgcgaaac
    17941 ccagcattat cgcatcgaat cccgttgcgc gctggccggc gcccgccggc ccgggccgcg
    18001 ccggcagcac cccccgcggc gacgggcctt gcggcgcggg cgcgccgccg ccggcgtggc
    18061 tgcgctatcc tcgcgcgacc gcctggcgcc gcgacgcgcc agccgccttg caggagcccc
    18121 catgtccacg ctgaacgaca cccatgatcc ccacctgacg agctgggtca cctcggccaa
    18181 cgacggccgc accgactttc cgatccagaa cctgccgttc gccatcttcc gccggcgcgg
    18241 cggcagcgag ccctggcgcg gcggcgtggc catcggcgac cagatcgtcg atctggcggc
    18301 ggcggcgcag gccggcctgt tccacggcga ggcccaggcc gcagcccagg cgggcgccga
    18361 acccagcctc aatcgattga tgtcgatggg ccaccaggct gcatccagct tgcgcaaggc
    18421 gctatcgaaa gcattgcaat cgggctcgcc ccagcaggcc gcgctgcaac cctgcctggt
    18481 gccgcaggcc gaggccgaat acgacctgcc ctgccgcatc ggcgactaca ccgatttcta
    18541 cacctcggtg caccacgcca ccaacatcgg caagcagttc cgccccgaca acccgctgct
    18601 gcccaactac aagtgggtgc cgatcggcta ccacggccgc tcctcgtcga tcggggtgtc
    18661 gggccaggcg tttcaccgtc cgcgcgggca ggtcaaaccg cccgacgccg acgcgccgag
    18721 cctcaaaccc agcgcccgca tggacatcga gctggagctg ggcgtgttca tcggcgcctc
    18781 caacgcgctg ggcgcgcccg tcgccctgga cgaggccgag gcgcatgtgt tcggcctggt
    18841 gctgttcaac gactggtcgg cgcgcgacat ccagccctgg gaataccagc cgctggggcc
    18901 cttcctgtcc aagaacttcg cctccaccat ctcgccctgg gtggtgacga tggaggcgct
    18961 ggcgcccttt cgcacgccgt tcacccggcc ggcggacgac ccgcagccgc tgccctacct
    19021 ggccagcccg gcccacagcg cggccggcgg cctggacatc gaactcgaag ccctgatcca
    19081 gaccgcccgc atgcgcgcgg cgggcagccc gcccacccgc atcacccaca ccaactaccg
    19141 ccacgcctac tggacggcgg cgcagatggt ggcgcaccac accgtcaatg gctgcaacct
    19201 gcagccgggc gacctgctgg gcacgggcac gctgtccggc cccacgctgg accaggcctg
    19261 cgcgctgatc gagctgacca ccggcggcaa gcacccgatc gccctgccca acggcgaaac
    19321 ccgcagctgg ctggaagacg gcgacaccat cgtgctgcgc ggctggtgcg caaggcccgg
    19381 cgcagcgcgc atcggcttcg gcgaatgcgt cggcaccgtg ctgccggccc gggactgaaa
    19441 ggggttccag ccgcggccgg ccggcaccgg cgcggcgccg ccgccctaca cttgggcgcg
    19501 tgaccgaacc gctgccgcgc cgcatcgccc acctggacat ggacgcgttc tacgcgtccg
    19561 tcgagtggct gcgctacccg cagctcaagg gcctgccgat ggtcatcggc ggcgggcggc
    19621 ggcgcgagga cgagctgatc gcgcggctca accggcagcg ccccgagcgc gtctggacgg
    19681 cggccgatct ggccgagatc ccggtcgact gctttccgct gctgcgcgac tacgtgggcc
    19741 gcggcgtcat caccaccgcc agctacgcgg cgcgccagtt cggcatcggc tcggccatgg
    19801 gcctgatgaa ggcggcgcgg ctgtgcccgc aggccatcct gctgccggcc gacttcgacg
    19861 aataccgccg ctgctcgcgc gccttcaagc gcgtcatcct ggacatcgcg ccggtgatgg
    19921 aagatcgcgg catcgacgag gtctacatcg acttcaccca ggtgcccggc ggccagcgcg
    19981 aaggcggacg cgtgctggcg cggctgctgc aaaaaagcat tgccgacgcc accggcctga
    20041 gctgctcgat cggcgtggcc cccaacaagc tgctggccaa gatggccagc gagttcgaca
    20101 agccgggcgg catcagcatc gtgcgcgaca gcgacctgca aagcctgatc tggcccctgc
    20161 cctgccgcaa gatcaacggc atcggcccca agaccgacgc gcgcctgcag cagcacggca
    20221 tccaccgcat cggcgatctg gcggcgcgcg agcgcgcctg gctgatcgcc cagttcggcg
    20281 ccagccacgg cgcctggctg cacgacgccg cctggggccg cgacgagcgc ccggtggtca
    20341 ccgacagcga gccggtcagc ctgagccgcg aaaccacctt cgagcgcgac ctgcacgccg
    20401 tgcacgaccg ggccgagctg ggggccgtct tcacccggct ggccgaggcg gtggccgccg
    20461 acctgcagcg caagggctat gccggcaaga ccatcggcat caagctgcgc ttcgacgact
    20521 ttcgcattgc cacgcgcgac cagacggtgg ccgagccgac gcaggatgcg cgccgcatcc
    20581 gccagatcgc cggccagtgc ctcaagcggg tcgagctgtc gcgccggctg cgcctgctgg
    20641 gcgtgcgggt cggctcgctg gtgcgcgccg atcggctgtc ggccgccagc gatgaggcgt
    20701 cgaatcacga tctgccgctg ttccagcaag cgccggcagc tcctgaaaac tgagcattcc
    20761 gagactgcgc gccgtcctgt tcaccggcac ggtttgacct gcccccggca aaagcttaca
    20821 gtttcctgac ggcacgacag gagacgatca tgctgaccgc gtggtgtctg aacgaattgc
    20881 aacgcatcaa ggcctggctg gtggcgcaca agccgagcca cccgctggag taccacagct
    20941 tcgacgccgt gctgaccctg tggatgatgg gctggatcgg ctggttgccg gtgctgctgt
    21001 tcgacgaggt gctgtgggcc tggccgctgt gtctgctggg catgtgggcg ccggcgctgt
    21061 acgtccgctg gcgcgcccgc gcccacgccg ccggccgtct ccgctgcgac tggctggggg
    21121 cgctggcgtc cactacagtg cgggtatgaa cattcctgcc ctgacctgtt ttcagctttc
    21181 cgtttccgac cacatcgccc acctggtgct cagtcgcccc gaggccatga acaccatggg
    21241 tccggcgttc tggcgcgagc tcgaccaggc gctggagctg ctgcagcgcg acggcgcggc
    21301 gcgcgcgctg gtgatctcca gcaccggcaa gcatttttcc gccggcatgg cgctcgacgt
    21361 ctttggcggc gccatccaga tggacgacgc ccaccccgaa gggcgcgccg ccatcgccga
    21421 cctgctggcc gacctgcagg ccaccttcac ccgactggag acgctgcgca tcccggtcat
    21481 cgtcgcgatc cagggcggct gcgtgggcgg cgcggtggac atggtgacgg ccgcctgcat
    21541 ccgctatgcc acgcaggacg ccttcttctg cgtgcaggaa atcaacatcg gcatggtggc
    21601 cgacgtgggc acgctgcagc gcctgcccaa gctggtgccg ctggcggtgg tcaaggagct
    21661 ggcctacacc ggccgccgca tgccggccgc gcgcgcacgc gaggtcggtc tggtcaacga
    21721 ggtgttcgac agcgccgacg cctgcctggc cgccgccatg cagtgcgcgc gcgagatcgc
    21781 cgccaagccg ccggtggcca tctggggcag caagcaggtg atccactacg cgcgcgacca
    21841 tacggtcgac gacagcctgc gccagatggg ctgggtgcag gccgccatct ggagcaacgc
    21901 ccacgtgcgc gaggccgtga cggccatgaa gcagcagcgc gccggcgcgt tcccgccact
    21961 ggccacgctg acgcgcttca ccgaccacga ctgatgcgag ctcgcattca acccagaaac
    22021 ggcggttgga cgcgcccgcc cgtgccgtgc tcggccaact gccgtttcta ggttcaatac
    22081 gacccatggg tcatggctcg cgccggcgct gccggtgtcg tgggtcaggt ctttgaacgc
    22141 caagccgcat gcgtcggatg cagggcggcc gctgcccgct cgagccgccc tactggcgcg
    22201 ccgtgcgcac gttgggcggc agcggcccgg ggtggctggt gcgccagggg ttgatgtcca
    22261 gcccgccgcg gcgcgtgtag cgggcgtaca cgctcagctt ggtgggccgg cagcgcgccc
    22321 ggatgtcggt gaacatgcgc tccacgcagt gttcatgaaa gtcgttgtgg cgccgaaagc
    22381 tgatgatgta gcgcagcaga ccggcctggt cgatctgcgg gccggcgtag cggatctgca
    22441 cgctgcccca gtcgggctgg ccggtgacgg gacagttgct cttgagcagg cggctggtga
    22501 gcgcttcttc caccggctgc tcgtcgaagg ccgcgctcag gcactgtggc gccggctcgt
    22561 cccagccgtc gacgtcgatg tccagacggt ccaggttgac gccgtcgagc tcgtgcaccg
    22621 cctcgcggtc gaagtcgtcc ggcgcgatca gccgcacgcc cgccgccgcc tgcaccgggc
    22681 cgccctgcca gatggcggcg ctgacgtcct ggcgcaggcg ccgcaccagc tcctcggtgc
    22741 ggtcgatgcg cgtgccgttg aagctgttca ggtacagctt gaacgacttg ctctcgatga
    22801 tgtgggtgct gtcgcacggc accatgatgt gggcgatggc cagctgcggc ttgccgcgcg
    22861 cattcagcca ggacagctcg taggccgtcc accagtcggc gccgaagaac ggcggctggc
    22921 cctggatgcc gagctgcgcc cgctgcggcg cgcgtgcgat cggaaacagc agcgtcgggt
    22981 cgtactgatc gctgtaggcg ctggcgcggc ccagcggtga atcgtgcggg gtggcgggag
    23041 tggtcatggg gtgtgaacct gggcggatgg cagaaacggc agcagcacgg ccagcacgcg
    23101 ccgcacgatt tccgggtggc cggcgggcgc cagatcgtgc gccatgtcgg ggatcagcgc
    23161 cggctgggca ccggcgatgc ggcgcgcggt gtcgtgggcg caggccggcg gcacccaggg
    23221 gtcgtcggcg ccgtgcacca ccagggtggg gcagcggatg cgcgccagct ggccggcgcg
    23281 cccgccgtcg gccaggatgg ccgccagctg gcgcgtggtg gcgtcgctgt ggggcgcgtg
    23341 gcgccgcgcg gtgcgggcca gcacgtcgcg cagttcggcg tcgctgggcg ggtagcgggg
    23401 gctggcgatg gcgcgcagga agttcaggta ataggccgcc agggcggcgt cgtcgccggc
    23461 gcgcggtttg cccgccgcca cgcgcagcac gctggcctgc ggcttcatca ggccgcgcgc
    23521 accgctgcag ccgctgatgc aggtcaggct ccacaggcgc gcgggcgcca gcagcgccag
    23581 gcgctgggcg atcatggcac ccatgctcag gcccaccacg tgggcccgcg ccacgcccag
    23641 ggcatcgagc acgcccagcg cgtcacgcgc catgtcgccc agggtgtagg gcacgcgcgg
    23701 gcgccgcccc agccaggcct gcgccgccac ccaggggatg gcgggcacgc cgtgggcgct
    23761 gaagtggctg gacagtccgg cgtcgcggtt gtccaggcgc agcacgcgcc agccggcatc
    23821 gaccagggcc tgcacgaacg ccgccggcca gtggatgagc tgcccgccca ggcccatgag
    23881 cagcatcacc acggggccgt cgccgcccga gtcctcgacc tcgatgcgga tcccgtttgc
    23941 ttcgatttgc atagctgctg gcgcttgctg ggtacgcgcg ggaggccgat ttgatgctca
    24001 accgatcaac ggaactcgcg ctcgcgcagc cacttggtgg cgatccactt ctcgccctcg
    24061 atgaccggcg agccgccgtg cagcgtcttg gtcgacgggt gcggccgctc gtagctgaag
    24121 aacaccgcgt tgccgcgctt gggcgccacc tcgaagaaca cgtccgggaa gctggtgcca
    24181 ccgcccttct cgggctcgct caggtacatc accaccgtgc caacgcgctg gccgccgcgc
    24241 ttgacgatgg tggcggtgcc gggctccttg gggtcgaagt agtcgtagtg cggcttgtat
    24301 tcggtgcccg gcaggtagtg cagcacctgg atgccttcgc cgttttccac cggccagcgg
    24361 atcagccgcg cgatgcgcgc ctcgatgcgc gccaccagct cgctttcgcc gcgctgaaag
    24421 aacatgccct ggctggtgcg gtcggggttg acctcggagc cgccggtctg ggtggccacc
    24481 gtcagcgaac ggctcaggcg cgggcgcgcg gcggcaatca gggcatcgca ttcctggtcc
    24541 gacagcaggc cgcccaggat caccacgcgc ggcagcgcca gcgaacccag caccgccacc
    24601 tggcggtcgc cgccgtccag gtagagcggc gaatcggcca cgtcgggctc gggcaccggc
    24661 accgccggcg gcaggccctg cgcctgcgcc tgctggtcca ggtggccgcg cagcgtgctt
    24721 tccatggcct cgatggccac gtcctcgttc cagcccgagc tcttcatcga ctgcaacacc
    24781 gcatcggcgc tgtggccggc acgcgcctga tcgacgatcc actggcgcaa ctcgggggtg
    24841 atggtctggg acatgggcaa cctcatgcgg agcggcgaaa caccagccgg tcgggcgtgg
    24901 acgccgccgg cgcgaacgca tagccttcca ggtcgaagcc ctgcagccgc tgcggcgagg
    24961 cgatccggtg ctgcgccgca tagcgcatca tcagcccgcg cgcacgcttg gcgtgcacac
    25021 cgacgacctt gaaggcgccg ccctggcgca cctcgaacac gcaatcgatc acgcgcgcct
    25081 gcagcaccca gcgatcgacc gccttgaagt actcctgcga cgccaggttg acgacgatcg
    25141 gcgccggctg accggccagc cgctggttca ggtggtcggc gatgcgcggg ccccagaact
    25201 ggtacaggtt gcgcccgcgc tcggtggcca gggcggtgcc catctccagc cggtagggcc
    25261 gcatgccgtc cagcgggcgc aacacgccgt acaggccgct caggatgcac aggtgctgct
    25321 gcagccagtc cagctgggag gcggtcagcg tgcgcgcctg cagcccttcg tagacgtcgc
    25381 cgttgaaggc cagcacggcc tggcgcgcct ggcgcgggcc gaagcgcggg cgccaggcct
    25441 ggtagcgcgc cacgttcagg ctggccagcg catcgctcag cttcatgagg gacgcgatct
    25501 gtgccggcgt ctgctggcgc agcacctcga tcagtgccgt agcttccggc acgaacagcg
    25561 gctgcgtgtg cggcagatcg gccggcagcg gcgtgtcgta gtcgagcgcc ttggcgggag
    25621 acaataggaa caacatgctg ccgattttgt accaggacgc gcagctggtc gcggtcgaca
    25681 agcccgccgg cctgctggtg caccgcaccg gcctggacgc cggtgaaacc cgctttgccg
    25741 tgcaatggct gcgccagcag atcggccggc cggtgtggcc ggtgcaccgg ctggacaagg
    25801 gcaccagcgg ggtgctgctg ctcgcgctcg acgccagctg cgccgccacc ctggcgcagg
    25861 actgggccgg cggcggcgtg cgcaagacct atgtggccat ggtgcgcggc tggccgcccg
    25921 cagcgggcct gatcgaccac ccgctggcgc gcctgccgga cgacgcccgc ggcgcgcgcg
    25981 gcctggaacg gcagccggcc ctgacccgtt ttcgcaccct ggcgcggctg gcgctgccgc
    26041 tgcccgaccg tggctttgcc cagacgcgct gcgcccgcat cgagctgcag cccgacaccg
    26101 gccgccgcca ccagctgcgc cgccactgca agcacatggc gcaccccatc atcggcgacg
    26161 ccacgcatgg caagggtgcg ctgaaccggg cggtcgcggc actgctgggc tcgcagcgtc
    26221 tgtggctgca cgcgcagtcg ctggtgctgc gccatccggt cagcggcgcg ccgctgcaca
    26281 tcgaagcggc gcccggcccc gaatgggcgc tgtgggcacc ctggacgccg acatggtgtg
    26341 atgcgccgcg tcatgcggca tcgcgttcgg acatatgacc catgaccgat gggtcatcga
    26401 ggcgcagcaa ggtgatccgt cattgcccat gggtcatgtt gaaagcgagt tcgtatcagg
    26461 cgggcgcggt gcgccgcatg cgccggccct gcaggcccca gaccagggcc atcagcagca
    26521 tgcccggcag caggaaccag ttcttgctcg ggcggtcggt cggcacctgc acgtgggcga
    26581 tgtcccagcc ctgttcgaag cccgatttgc gcgcctgcga gccgaagcgc accgccccca
    26641 cctgcaccgc gtcgcccagc gtcatcagcg tcaggccggc gtcggccagg cgcgcgcgac
    26701 cgtccggacc ttgcgcgccc agttgcaccg ccacggtctt ctcgacctcc tcgccttcca
    26761 gcgtggtgcc cttgatgacc agcaccagcg tgccttggtc gggcagcgcg cgcgccacct
    26821 ccagcacctg ggccgccggg acacgggtgt gttccggcgc cagccggtcc atgaagaaac
    26881 ccggccggaa cagcatcacc acggccagcg ccagcagcgc gaattcccac caggtcaggc
    26941 ggatgcgcag ccagttcatg gtgagcgcgg caaagcccag catggccagc gtgcaggccg
    27001 acaccaccag caccagctcg ccccagccat gcacgtcgag cagcagcagc tgcggattga
    27061 agatccagat gaagggcagg atcacggtgc gcagcgcgta cagcgagccc tggatgccgg
    27121 tctggatggc gtcttcgccc gagatggccg ccgccgcgaa ggtggccagc cccaccggcg
    27181 gcgtgatgtc gcccatgatg ccgtagtaga agacgaacag gtgcaccgcg atcagcggga
    27241 tcaccagccc cgcctgggcg cccagctcga ccaccaccgg cgccatcagc gtggccacca
    27301 gcacgtagtt ggcggtggtc ggcacgccca tgcccagcac caggcagacg aaggcgatga
    27361 acagcagcat ggcgatcacg ctgccctgcg agacgaattc gacgaactcg gtcatgcgca
    27421 ggcccagtcc ggtgagcgtg atggcgccga cgatgatgcc ggcggtggcg gtggccacgc
    27481 cgatgccgat catgttgcgc gcgccgtcgt gaaagccgtg caccaccgcc tgcccgccct
    27541 cgcgccaggc gcccggagtg cgcacaccgc gcagcaagcc cagcagcggg cgctgggtca
    27601 gcatcagcac aaccagggtg gcgcaggccc agaacgccga cagcgcgggc gacatttcct
    27661 cgaccatcag gcaccagatc agcacgccga tcgggatcag gtagtgcagg ccggcgcgca
    27721 ccgtgggcca ggtttccagc cgctgcgggt ggtgcacgtc gatgtcttcc ggcaggtcgg
    27781 ggctgacggc ggcctggcgc agcgccacca ggtacagcgc cagcagcgtg ccgcccaccg
    27841 cccagggtgc ggcagcgccc agggcgctct tgaagaactg cagcaggtgg tacagcgccc
    27901 ccaccaccgc cagtgcgccg ctgctgccga tcagcgtgcg catcagccgc gcgcgccagg
    27961 ggcgcggcgc ggcggcggcg gccggctgga tgccgagctt gagcgcctcc aggtgcacga
    28021 tgtagagcag ccccaggtac gacagcgagg ccggcagaaa ggcgtggcgc accacgtcgg
    28081 agtacggaat gccgacgtat tcgaccatca ggaaggccgc cgcccccatc accggcggca
    28141 tgatctggcc gttgaccgag ctcatggttt cgatggcgcc ggccttgacg ccgccgtagc
    28201 cggccttctt catcatgggg atggtgaaga tgccgcccga caccacgttg gacaccgacg
    28261 agcccgaaat catgccgttg agcgccgacg acaccaccgc caccttggcc ggcccgccgc
    28321 gcaggtggcc cagcgcggca aagctgacct gcatcatgta gttgccgccg ccggcgcggt
    28381 ccagcaaggc gccgaacagc acgtagatga agatggtgct ggccgacacg cccagcgcga
    28441 tgccgtacac gccctcggtg gtcagccaca tgtgcgacag caggcgcgac agcgacgcac
    28501 ccttgtgcgc cagcacctca ggcatccagg ggccggccac gcagtaggcc agaaacagcg
    28561 ccgccagcgc cgccatcggc cagccgaccg cgcgccgcgt ggcctccagc agcagcagca
    28621 ggccggcgct ggcggtgacg atgtccatga cgctgggctg gccgggccgc gtggccagct
    28681 cgcggtagaa cagcatcagg taggcaccgg caaaggcgcc ggcgatggcc agcccccaat
    28741 cgaccagcgg cacatggcgg cgcggcgagg agcggaacgc cggccaggcc agaaacgcca
    28801 gcgccagcga aaacgccaga tggatggcgc gcgcctcggt gtcgttcagg atgccccagc
    28861 cgagggcaaa gggcagcggc gaggcgtacc agaactggaa caccgcccag gccagcgcca
    28921 gtccgaacat cagccgcgcc gtcaagccac tgggcttgcg cccgccggtg tcggtttcgg
    28981 ccaccagctg ctgcagcgcc tcgggtgctt tttcgatgtc ggtggccatg gcgtgtctgt
    29041 cggttcacta tgttatggat agctgccggc gcttgatcgg cgggcgctgg agccactaca
    29101 tgccatgaac ggccgggcca tcaaaaaggg cgaagtcgcc ttcgccctga tcgccgtgca
    29161 tgtgcgtgcg gggcacggcc cggccgcgcc atggcttact tgatccagcc cttttccttg
    29221 tagtagcgcg cggcgccgtc gtgcagcggg gcgctcaggc cgttcctgac catgtcctcg
    29281 ggcttgaggt gggccagcgc cgggtgcagc ttcttgaact cgtcgaaatt gtcgaacacc
    29341 gccttggtga cggcgtagat ggtgtcggcc ggcgtcttgc tggaggccac caccgtagcc
    29401 agcacgccgt aggtgggcgt ggcctgcggg ttgttggggt acagcccggc cgggatgctg
    29461 accttggcgt agtacggctt gtcggccacc agcttgtcca ccaccgggcc ggtcagcggc
    29521 accagcttgg caccgcagct ggtggtcggg tcctggatgt tggcgctggg gtggccgacg
    29581 ccatagaaga agccgtcgat cttgccgtcg cacacggccg ggccgtgctc gtcggccttc
    29641 agctcggagg ccagcgagaa gtccgacagc ttccagccca tggcggcgag cagctcctcc
    29701 atcgaggcgc gcgtgcccga gccggggttg ccgacgttga agcgcttgcc cttgaagtcc
    29761 tcgaacttgg tgatgcccgc ttccttgcgc gccaccacgg tgaacggctc cgggtggacg
    29821 gcgaacacgg cgcgcagatc ggacaccgcg ccaccgtcct tgaactggct caggcccttg
    29881 gtggcgttgt actggacgtc ggactgggca aagcccaggt ccagctcgcc ggccttgatg
    29941 gtgttgacgt tgaacaccga gccgccggtg gattcgaccg agcagcggat gccgtgcttg
    30001 gcgcggtcct tgttgaccag gcggcagatg gcgccaccgg cggcgtagta cacgccggtg
    30061 accccgccgg tgccgatggt gacgaacttc tgctgcgccg acgccggcgc cgccagcgcg
    30121 gcggtggaca gagcggcagc ggcggccagg gtcttgagaa cggggcgaag gctcatgcga
    30181 caaggtctcc tgtgaatgaa tggggcatgc tcagccctgc gcgatcgccg catcgacggc
    30241 cgcggccagc cgttcggcga tcagcgccag ttcgtcatcg gtggcgatga acggtggcgc
    30301 cagcaggatg tggtcaccat agcgcccatc gaccgttccg cccatgggat aaaccagcaa
    30361 tccgcgcgcg aacgcctcgc tcctgacgcg cgcgtgcagg cgccgcgcgg gatcgaacgg
    30421 ggttttgccg gcgcgctcgg cgaccagctc gatgccccag aagtaaccac gcccgcggac
    30481 ctcccccaca tgcgggtggt gggcaaacct ttcggccagc agcgcttgca ggacctgcgc
    30541 gccgcgctgc actttttgca gcaatccatc gcgctcgatg acctgctgca cggccagcgc
    30601 ggcggcgcag gcgacggcgt ggccgaggta ggtgtggccg tgctggaaaa acccgctgcc
    30661 ggccgacatg gcggccacga tccgctgttg cgccagcacc gcgccgatcg gctggtagcc
    30721 gccgcccagc cccttggcca cggtcatcag gtcgggcacc acgccgtcct gctggcaggc
    30781 gtgcagggtg ccggtgcggc ccatgccgca catcacctcg tcgaggatca gcagcacgcc
    30841 gtggcggtcg cacacctcgc gcaccgcctt gaagtagccc ggcaccggca ccagcacgcc
    30901 ggcggtggcg ccgcccacgg tttcggccac gaagcccagc accttgccgg ggccgacgcg
    30961 gcagatcgcc tcgtccagct cgcgcgccag gcgctggccg tactgctcgg gcgtttcgcc
    31021 cggctgctgg tcgcggtagg ggtagcaggg cgacacgtgg gtcacgtcca ccagcagcgg
    31081 cgcaaactgg cgccggcgcc attcgttgcc gcccaccgcc agcgcgccca gggtgttgcc
    31141 gtggtagctc tggcgtcggg cgatgaagtg gctgcgctcg ggctggccga tttccaggta
    31201 gtactggcgc gccatcttga gcgccgcctc caccgcctcc gagccgccgc tgacgaagta
    31261 ggcgtggctc atgcccgccg gcgcggtgcg caccagctgc tcggccagct gctctgccac
    31321 ctcggtggtg aagaagctgg tgtgggcgta ggccagctgg tcgatctgcc ggtgcatggc
    31381 ggccagcacg tccgggtgac cgtgccccag gcaggacacg gcggcgccgc ccgaggcgtc
    31441 gaggtagctg cgcccggcgg catcgtgcag ccacacgccc tggccgccca cggcgaccgg
    31501 gggcgtgtgg tgcaggtgcc ggtgcatcac gtgcgtggtg ctcatggccg tcggtctcca
    31561 ggggttcggc gcgcgccggc gctcactgcg ccgtccagcc gccgtcgttg gcccaggcct
    31621 ggccgcgcac gttggccgcc gccgccgagc agaagaacac cgccatggcg cccagctgtt
    31681 cgggggtggt gaactgcagc gagggctcct tctcgcccag caactcgcgc gtggcctgct
    31741 cggtgctgat cttctcggcc gcggcgcgcg cgtcgatctg tttctgcacc agcggcgtca
    31801 gcacccagcc ggggcagatg gcgttggcgg tgacgccggt ggtggccagc tccagcgcgg
    31861 tgaccttggt caggccgacg atgccgtgct tggccgccac gtaggccgac ttgtccttcg
    31921 acgccaccag gccgtgcacc gaggcgatgt tgatgatgcg gccccagttg cgcgccttca
    31981 tgtgcgggat ggccagccgc gtggtgtgga acgccgacga caggttgatg gcgatgatgg
    32041 cgtcccagcg ctcggtcggg aagccctcga tcggcgacac atgctggatg ccggcgttgt
    32101 tgaccaggat gtcgacgccg ccgaacttct cggccgcgaa ggccatcatg gcctcgatct
    32161 cggccggctt gctcatgtcg gcgccgtggt aggcggtctg cacgccgcag gcctcgatct
    32221 gccggcgcgg tgcgtcgacg tcaccgaagc cgttcatgac gatatgggcg ccctgcctgg
    32281 ccagttcgat ggcgatgccc aggccgatgc cgctggtcga gccggtgacg agggcggtct
    32341 tgcctttcag agacatgagg tgatcctttc gtagctggaa catgaacacc ggacgcaaag
    32401 gacgcgcaac gtgcgcaaac aacgcgaaag aaaccaggcg ttctctttcg cgtccttcgc
    32461 gtgatcttcg cgcccttcgc gtccggatgg attgaagtgg ctgccagccc tttagctgct
    32521 tgcgcgagaa gctatggtta ttggagcagc ggaacgccgg tcttgccttg cacttcgtcg
    32581 aagctgacgt ccggcgccag ctcgaccacc ttcaggccgc gatcggtgac gtcgaacacg
    32641 cccaggtcgg tgatgatgcg gtcgaccacg ccgacgccgg tcagcggcag cgtgcactgg
    32701 ggcaggatct tcatgtcggt gctgccgtcc ttctttttgg cgacgtgttc catcagcacg
    32761 atcacgcgcg ccacgccggc caccaggtcc atggcgccgc ccatgccctt gaccatcttg
    32821 cccgggatca tccagttggc cagatcgccc ttgtcggtga cctgcatggc gcccaggatg
    32881 gacaggttga tcttgccgcc gcggatcatg gcaaagctgt cgtggctgcc gaagatcgag
    32941 ctgcccttga tggtggtgac ggtctgcttg ccggcgttga tgaggtcggc gtccacctcg
    33001 tcggccgtgg ggaacgggcc gatgcccagc atgccgtttt cggattgcag ccacacctcc
    33061 ttgtcggcgg gcacgtggtt ggccaccagc gtcgggatgc cgatgccgag gttgacgtaa
    33121 aagccgtcct gcagctcctg cgccgcgcgc gcggccatct ggtcttgggt ccaggccatg
    33181 tctcgatctc cttgattact tgtgctcgcg cagggtgagc ttttcgatgc gcttttcggg
    33241 cgtggggttg tgcacgatgc ggtgcacgta gatgccgggc aggtgaatgt cgtcgggcgc
    33301 cagttcgccg ttggcgacga tgcgctcgac ctcgacgatg cacagctttc ccgacatcgc
    33361 cgccgccggg ttgaagttgc gcgccgtcag gttgaagcgc aggttgccgc tgcggtcggc
    33421 cacgtcggcc ttgaccaggg ccacgtcggc caccagcgag cgctccatga cgtaggtctg
    33481 gccgtcgaac tcgcgcagct ccttgccctc ggccaccagg gtgccgacgc cggtcttggt
    33541 gaagaaggcc ggaatgccgg cgccgccggc gcgcagcttc tcggccagtg tgccctgggg
    33601 cgtgaattcc agctccagct caccggccag gaactggcgc tcgaactcct tgttctcgcc
    33661 cacgtaggag ctgatcatct tcttgatctg gcgcgtctcc agcagcttgc ccaggccgaa
    33721 gccgtccaca ccggcgttgt tgctgatcac cgtcaggttc ttcacgccgc tgtcgcgcag
    33781 cgcgtcgatc agcgcctcgg ggatgccgca caggccgaaa ccgcccacgg ccagttgctg
    33841 gccgtcgtgc accacgccct tgagcgcgtc ggcggccgat ggataaatct tgttcacggt
    33901 ctctctccag ttgcaaatat caaacgaaca gcaggctcag gctcggcgtg aacgatacta
    33961 cgtaaacgac tacgtagtat ttcgtaatgg cgatccctgt ccctgcggcc ggcgacccgg
    34021 cgctggcgcc accggactac cggctggcct ttcaactggc gccggtcggc ctgattctct
    34081 cgcgccagcg ccacatcgtc gattgcaacg accgcgtgtg cgacatcttc ggcgccgagc
    34141 cggcgcaact gatcggccag agctttcgca ttctgtatcc cagcgccgac gaatacgagc
    34201 gctttggcgc gcgcatcgcg ccgatcctga accgcgacgg cctgtacgcc gacaaccgcg
    34261 tgatgcagcg ggtgggcggc ccgcaggccg gcgaggcctt ctggtgccac gtcaccggcc
    34321 gcgccatcga ccgccgcgcg ccgcacgccg ccggcatctg gagcttcgag gacctgagcg
    34381 cccagcgacc gatgggcgcc gtgctgaccg cgcgcgagcg cgaagtcgcc gcccagctca
    34441 cgtgcggcct gaccagcaag gagatcggcc gcgccctggg cctgagccac cgcaccgtcg
    34501 aagtgcaccg cgccaagctg atgcgcaagt accgcgccgg caccacggcc gagctggtgc
    34561 aaaagctcat gggcgggctg tgagccgaat cgccgcgcct tgacaaacta tcaaatcgat
    34621 agctgcttgc gcttgtctgg caagcgccag agcctatttt ttgtcggatt gaccagggcc
    34681 gccgtccacc acctcggcgg cactgcgaaa ggcctccacc gcgcacggca cgccgcagta
    34741 gatgctggcg tgcagcagca cctcctgcag ctcggcgacc gtggcgccgt tgcgcagcgc
    34801 gccgcgcaca tggcccttga gctcgtgctg gcggcccagc gcggtcagca tggccaccgt
    34861 caccaggctg cgcgtcttca ggtccagccc cggccgctgc cacacgtcgc cccaggcgtt
    34921 gcgggtgatg gcgtgctgca tcggctcggt gaacggcgtg gcgctggcga acgcgcgatc
    34981 gacgaaatcg tcgcccatca cctggcggcg cttcttcagg ccgtgctgga acgccgggtc
    35041 gtcgggcagt gtcatggcgg ggtctcctgg aggcaaaggc cgtcagcata atgacccgca
    35101 cccccaccag accaggagag ccccatgagc cgctaccccc tgcccagcct cgacagcctg
    35161 cccgaagaca tccgcgcccg catcctcgaa gtgcaggcca aggccggctt cgtgcccaac
    35221 gtatttctgg cgctggcgcg ccggccggcc gaatggcgcg ccttcttcgc ctaccacgac
    35281 gcgctgatgg agcccgaaag cgccggccgc agcagccggc tgagcaaggg cgaccgcgaa
    35341 atgatcgtca ccgccaccag cgccgccaac ggctgcctgt actgcgtggt ggcccatggc
    35401 gcgttgctgc gcatctacga gaagaaaccc ctggtggccg accaggtggc caccaaccac
    35461 cgcaaggccg acatcagcgc gcgccagcgc gccatgctcg acttcgccat gaaggtcagc
    35521 cagcacccgc gcgagctggg cgaagaggac ttcgcccccc tgcacgccca cggcttcgac
    35581 gacgaagaca tctgggacat cgccgccatc gccgccttct ttggcctgtc caaccgcatg
    35641 gcctcgttca gcaacatgac gccgaatgcg gagttttacc tcatggggcg ggtgccgagg
    35701 gaaaa
//
LOCUS       DAOQTZ010000011        35600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1387_length_35600_cov_13.326853, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000011 DAOQTZ010000000
VERSION     DAOQTZ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 35600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35600
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1387_length_35600_cov_13.326853"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1544
                     /gene="ccoN"
                     /locus_tag="PKN51_02625"
     CDS             108..1544
                     /gene="ccoN"
                     /locus_tag="PKN51_02625"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNT84024.1"
                     /translation="MTFANTQATVYNDKVVRQFAIMTIVWGVVGMLVGVIIAAQLAWP
                     ELNFGIPWLSYGRLRPLHTNAVIFAFGGSGLIGTSYYVVQRTCQVRLFSDKLAAFTFW
                     GWQLVIVAAAISLPLGYTTSKEYAELEWPIDVLITLVWVAYAVVFFGTVGTRRVRHIY
                     VANWFYGGFILAIALLHVVNSAELPVGLMKSYSAYAGVQDAMVQWWYGHNAVGFFLTA
                     GFLGMMYYFIPKQAERPVYSYRLSIVHFWALIFTYMWAGPHHLHYTALPDWAQSVGMI
                     FSLILLAPSWGGMINGIMTLSGAWHKLRDDPILRFLIVSLSFYGMSTFEGPMMSIKTV
                     NALSHYTDWTVGHVHSGALGWVGLITMGSMYYLIPRLFGQKQMHSVKAIEVHFWVATV
                     GIVLYIAAMWIAGVMQGLMWRAVNPDGTLTYTFVESVKATYPFYVLRLMGGLLYLGGM
                     LIMLWNTIKTATAGRVVPVQIPAVAAHA"
     gene            1562..2179
                     /gene="ccoO"
                     /locus_tag="PKN51_02630"
     CDS             1562..2179
                     /gene="ccoO"
                     /locus_tag="PKN51_02630"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNT84025.1"
                     /translation="MAQEKVTGHAKVETSNFLMIVLILLVVSIGGLVEIVPLFFQKST
                     TQPIEGLKPYTALQVVGRDVYVREGCYNCHSQMIRPFRAEALRYGPYSVAGEFVYDHP
                     FQWGSKRTGPDLARVGGRYSDDWHRAHLNNPRDVVPESNMPAYPWLERTAANAGTIQA
                     HMRGLRTLGAPYTDEEIAKAPEEVKGKTEMDAVIAYLQVLGIHRK"
     gene            2200..2340
                     /locus_tag="PKN51_02635"
     CDS             2200..2340
                     /locus_tag="PKN51_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNT84026.1"
                     /translation="MDINLLRSIVTVLSFVLFIGIVVWAWSARNRERFDEAAQLPFEQ
                     QD"
     gene            2374..3261
                     /gene="ccoP"
                     /locus_tag="PKN51_02640"
                     /pseudo
     CDS             2374..3261
                     /gene="ccoP"
                     /locus_tag="PKN51_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016722100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            3355..3651
                     /locus_tag="PKN51_02645"
     CDS             3355..3651
                     /locus_tag="PKN51_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT84027.1"
                     /translation="MVKFFFATGKADLAPEADKALADILEGVKAGKKAVISGFVDSTG
                     NAEQNAELAKQRAIAVRDQLKALGVAEDQVELKKPANIDAGADAQARRVEVSLG"
     gene            3940..5307
                     /gene="ccoG"
                     /locus_tag="PKN51_02650"
     CDS             3940..5307
                     /gene="ccoG"
                     /locus_tag="PKN51_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNT84028.1"
                     /translation="MVSLYQAHKKVYPRSVTGLFAKWRWFFVFATQIFFYGMPWLQWG
                     ERQAMLFDLEARRFYIFGLVLYPQDVIYLTAILIISALSLFLFTAVAGRQWCGYACPQ
                     TVYTEIYLWFERMIEGDRSARMRLDAAPMSAAKFGRKALKQAVWIAFGLWTGFTFVGY
                     FTPIRELAQHVLQWNLGAWEIFWIFFYGFATYGNAGFMREQVCMYMCPYARFQSAMFD
                     KDTLIVTYDAKRGEPRGGRSKSADPKALGLGDCVNCTLCVQVCPTGIDIRNGLQYECI
                     SCAACIDVCDEVMDKVGYPRGLIRYSTEHALEQGWGRKEILRQVLRPRVLIYVAILSA
                     VVVALIASLVLRTPFKVDIVRDRATLARLVDGNQIENVYRIQVMNATEKPQTFQLSVE
                     DLPGLEIASDKSVTVDGAQSLWVPVQLRLPYGSASSGSHTIHFRVTAPGVGEVREKSI
                     FMVPR"
     gene            5313..5606
                     /locus_tag="PKN51_02655"
     CDS             5313..5606
                     /locus_tag="PKN51_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein FixH"
                     /protein_id="HNT84029.1"
                     /translation="MSDPASTPPSPWWKFGHMWLVIAGPLIVVLASIVTIYLAVRTPD
                     PVYGDASRGAARSSQAKDGGDAGRMSPAMQARNHAATGGVARPAPNHGTGPNQ"
     gene            5644..5901
                     /locus_tag="PKN51_02660"
     CDS             5644..5901
                     /locus_tag="PKN51_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84030.1"
                     /translation="MLRERLMWIAWPAFLMAAVLEFMVFAVLDPQALTLFGEPSGWSR
                     EGVYTVTFFIFWAVMMLSGALTTLLSKSPFDVNRRQSRALR"
     gene            complement(5988..6084)
                     /gene="ffs"
                     /locus_tag="PKN51_02665"
     ncRNA           complement(5988..6084)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKN51_02665"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6090..6332)
                     /locus_tag="PKN51_02670"
     CDS             complement(6090..6332)
                     /locus_tag="PKN51_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84031.1"
                     /translation="MMDVFESHDTIVIQTHADPRIAALVGALTDCIHPRAARLKARLA
                     ADPDDEQLGQLRAEVLNLVAMSFGLAEAERRLQAVQ"
     gene            6503..8458
                     /gene="dnaX"
                     /locus_tag="PKN51_02675"
     CDS             6503..8458
                     /gene="dnaX"
                     /locus_tag="PKN51_02675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNT84032.1"
                     /translation="MSYLVLARKYRPRNFSEMVGQEHVVQALTNALTQQRLHHAYLFT
                     GTRGVGKTTVSRILAKSLNCTGADGQGGITATPCGVCPACADIDAGRFVDYTELDAAS
                     NRGVDEVQQLLEQAVYKPVQGRFKVFMIDEVHMLTGHAFNAMLKTLEEPPEYLKFVLA
                     TTDPQKVPVTVLSRCLQFNLRPMAPETVLDHLTRVLAAEAVAAEPAALRLLARAARGS
                     MRDALSLTDQAIAFGSGELRESAVRQMLGSVDRSVVFSLIGALADGDGRTVVTLSEQL
                     RASGHSAAQTLEDMASVLQRMAVQQAVPDLAVDASDPDAQETARLAARLPADETQLLY
                     SLCIHGRSELGLAPDEYTGLTMALLRLLPFRQPVPTQAQTVSATAEKKSLKTPEASPA
                     PQGASAPPAKAPAPAPVAASDDPAAAEQGAAELGAAADARPQPVTERDAELDTQGAPP
                     ARPVPAPRPQPALQPLPVRPSNGTRFQEQNQPPAPDHIAPVAPETIAIQVREGDQEPA
                     PAPRLRVDAPPPPVVPGAEGDFWHALVQELVAREAVTALVRELALQSQLVARTDRQWT
                     LRVESESLAQSGVRERLQAALADAGHCVRLQIDSGPVSDSPARRNAIHATRRLKAAEA
                     LLLADPFVQEMMRDFGAKIVPGSIKPL"
     gene            8579..8914
                     /locus_tag="PKN51_02680"
     CDS             8579..8914
                     /locus_tag="PKN51_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNT84033.1"
                     /translation="MFNKGQLAGLMKQAQAMQDNLKKAQDELALMEVEGESGAGLVKV
                     LMTAKHDVKRVTIDPSLLADDKDMLEDLVAAAFNAAVRKAEELSAEKMGKLMPAGMPG
                     LPGGMKFPF"
     gene            9059..9649
                     /gene="recR"
                     /locus_tag="PKN51_02685"
     CDS             9059..9649
                     /gene="recR"
                     /locus_tag="PKN51_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNT84034.1"
                     /translation="MSDTHSLDTLIQALRRLPGVGQRSAQRMAFHLLQHDREGAQQLA
                     RALHEAVGQVRHCARCHTFTEAEVCATCSDPRRDASQLAVVETPADQAALERTGAFRG
                     RYFVLMGRLSPLDGIGPRDIGVQRLIERACDGVVREVILATNFTAEGEATAHLIGQAL
                     RSRGLAVTRLARGVPAGSELEYVDLGTIAHALVDRR"
     gene            9656..10411
                     /locus_tag="PKN51_02690"
     CDS             9656..10411
                     /locus_tag="PKN51_02690"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNT84035.1"
                     /translation="MSLSASILAARQLLQEAAHIAVLSGAGISAESGVPTFRDAQTGL
                     WAHLRPEDLATEDAFRRHPQRVWDWYAERRASLAEVQPNAGHRALAEFQHRHPGRLTV
                     ITQNVDGLHQRAGSTGVLALHGSLAADRWLDPCPAGRGPTACDVARAEPGRPPRCAQC
                     GNWVRPAVVWFGELLPEDQLRQAEQAASRCDAMLVVGTAGAVYPAAGLAHQARAAGAR
                     LIVVNPHPSELDGLAQLRLVGGAAQVLPPLLDF"
     gene            complement(10555..11244)
                     /locus_tag="PKN51_02695"
     CDS             complement(10555..11244)
                     /locus_tag="PKN51_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HNT84036.1"
                     /translation="MLIHLKNLLSADEVAHARRLLLADDAPWVDGARSAGGQAVQRKS
                     NRQLAQDSPSAATLRELVLAALRRDPVFFSAALPRRIFNPLFNRYEGAANHYGQHVDG
                     AVLHSQTSGEWVRSDLSCTVFLTEPDDYDGGELVVHDTFGPQAVKLPAGDAVLYPGTS
                     VHEVRPVTRGARIASFLWIESMVRGQEQRRLLFEMDMALLALRQRHGETDEAIRLTGT
                     YHNLLRMWAST"
     gene            complement(11313..13601)
                     /locus_tag="PKN51_02700"
     CDS             complement(11313..13601)
                     /locus_tag="PKN51_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNT84037.1"
                     /translation="MPPIRSKSKAKKPESLIQQAPNAPVFEADSTPLLPLGAMLLAGS
                     LSTAALAQTPASAPVAGTLGTVTVRDSATTDGAATTSKSQLRATRTEIGKGNQELRDI
                     PQSVTVMTERLIQDRNLDDFRDVLRATAGVTFQAGETGEEDVRLRGFSLGQAGDIYRD
                     GMRDAPLYERDTFNDDRVEVIKGSASMLFGKGSTGGVVNQVSKQPFLMNQHEASVTAG
                     TGRMGRVQGDFNWQTGQDSAFRLNAMAHDASNWGARQRKIGVAPTFRWGIGTRDEFTV
                     GFYHLDIDGRPNYAHPWIIGADGTIHPTLPAKNFYGLKNDHLDTSATYATLGHTHRFD
                     HGGELKTRLRAGRYERDLLGSPIGFAQPRPTALSDITASTVLTRSSKGRIGKSDVVML
                     QSDYSHSFELGGRKHSLITGIDVYDESAKRNNNYPLPNTPARPNTTVGTPNDGAWVTD
                     TRVTPVPYNSFDSRSIGAYLQDTVSLTDTVKLVGGLRFDHFKGSYRNADGSLSNARTD
                     NLWSPRLGLLYQPDEASSYYISYGTSFNTSGDAYQYGVLINPNTGRSAKTPPEKSRNI
                     EIGGKWDLFDKRMLAGAALFYSEKYNERNTDPDTAATQELLSGKRHAAGMEFNLAGRI
                     TPAWELFWNHTWIPKAKIDKSNVVLNPAGTGAQVQGDRPGLTPRHSGSVWTTYRVMPQ
                     LRLGLGANYRSSQNPEGQRTLKAKGFVTFDAMAEYSFTEATTLKLSVTNLSNKLYADG
                     LYRGFYQPGAPRRVELTLKHLF"
     gene            complement(13820..14296)
                     /gene="bfr"
                     /locus_tag="PKN51_02705"
     CDS             complement(13820..14296)
                     /gene="bfr"
                     /locus_tag="PKN51_02705"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNT84038.1"
                     /translation="MQGDAKVIAHLQAQLKNELTAINQYFLHHRMLKHWGLERLARKE
                     YEESIGEMKHADRLMDRILMLDGLPNLQDLGKLMVGEDVPEILRCDLKLEQAAQATIK
                     DGIAHCESVRDYVSRDLLQDILDDTEEHIDFLETQIELLDKVGLQNYLQSQMGAPS"
     gene            14475..16283
                     /locus_tag="PKN51_02710"
     CDS             14475..16283
                     /locus_tag="PKN51_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNT84039.1"
                     /translation="MTDTRPAAQPQPEPQRRRARDRLKGRQPEAGARDEVRALIGTPG
                     PDGHRRDLLIEHLHRLNDRYHGLFERHLVALAAEMRLSMAEVYEVASFYHHFEVRKDG
                     EAAPRLTVRVCTSLSCQLAGADALLARTRELLGAEVQVLAAPCIGRCEQAPAALVGPH
                     GVGQASAERLVQASNRALALDGPAPTAIKPIVFDAYTQAGGYTLAQAVARGEPGHEAI
                     IAALEHAGLRGLGGAGFPAGRKWRIVRDLARSAERRAAPSEAAAPSGGSGPRAAGERG
                     GMTYLAINIDEGEPGTFKDRWYLERDPHRFLEGLLIAAQVVGVQGVYIYLRDEYPECR
                     TILTQALADLRAAPGLRELLPPTELRRGAGAYICGEESAMLESIEGKRGQPRLRPPYI
                     AQVGLFGRPTLEHNFETLYWLRDILQRGPDWFAAQGRHGRQGMRSFSVSGRVRQPGVK
                     WAPAGITLRELVDEYCGGMLDGHELYAFLPGGASGGIFPARLADVPLDFDTLQPHGGF
                     IGSAGVIVLSQHDKARDAALNMMRFFAAESCGQCTPCRVGTAKAARLMEAPVWDAATL
                     TDLSQVMQDASICGLGQAAPNPMQCVQRYFAHEVEA"
     gene            16280..19126
                     /gene="fdhF"
                     /locus_tag="PKN51_02715"
     CDS             16280..19126
                     /gene="fdhF"
                     /locus_tag="PKN51_02715"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNT84040.1"
                     /translation="MTPALACIAFELDGQPATAFEGETILKAAERHGVQIPRLCFTDG
                     LRADGNCRACVVEVDGERTLAPSCCRAVQPGMKVRAASERARRAQDMVLELLLAELPA
                     AGFKWLDDDATQPHGELSQWASQRKVGVRQQLQNLQRPRPTPDLSHPAMAVNLDACIQ
                     CTRCVRACREAQVNDVIGLAGRGAEARIVFDLDDAMGASSCVACGECVQACPTGALMP
                     KTHIGPQRVDRSVDSVCPFCGVGCQITYHVRDEKIVSVSGRNGPANAGRLCVKGRFGF
                     DYAASPDRLTRPLIRIAGVPKDPAHCQQPPDWRTVFREASWDEALDAAAGGLRALKSR
                     HGPRALAGFGSAKGTNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAALLEGVGSGA
                     VSNPVRDVEHAELILVIGANPTANHPVAASWMKNAARRGTRIVLADPRATDIARHCWR
                     VLQFRPGSDVALLNALLHVIVTEGLGNESFLRQRADHFAALAANVQGYSPEAMAPICG
                     IPAETLREVARAYATARSAMILWGMGVSQHVHGTDNARCLIALATCTGQLGRPGTGLH
                     PLRGQNNVQGASDAGLIPMMYPNYQRVDSPDARTWFEDFWGQPLDPEPGYTVVEIMRQ
                     ALAADDDPHKVRGMYIMGENPAMSDPDLNHTRHALASLSHLVVQDIFLTETAWLADVV
                     LPASAWPEKTGSVSNTDRMVQLGRQALAPPGEARADLWIIQQLAQRLGLPWNYENDSC
                     SRLPDKRQGPFFLEEPHGVAAVYEEMRRAMHGAIAGIGWDRLVRESSVTYPCLSEDDP
                     GQPIVFTERVPTPNGRVQLVPADIIAPDERPDADYPMVLITGRQLEHWHTGAMTRRAV
                     VLDALEPAPTVSMHGDDLARLGLVAGELVRVSSRRGRVCLTARRDDGTPVGGVFIPFA
                     FREAAANLLTNAALDPFGKIPEFKYCAVRVEPLA"
     gene            19123..19953
                     /locus_tag="PKN51_02720"
     CDS             19123..19953
                     /locus_tag="PKN51_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNT84041.1"
                     /translation="MTVHLTRASAPLTERVLAVDELGQAVEVSIPAERALTLYVDKRE
                     LVTLMTLGAHPEWLVLGYLRNQRLLAGPAELASITVDWDAGAAAVRTRHGIADLEMRT
                     RRRTVTSGCGQGSVFGDLMADLEALDLGAGRLALDDLYGLLNAIRLQESTYKSAGSVH
                     GCALFEGQRMLLFVEDVGRHNAIDTIAGWMWLQGDGGGLRGDDKVFYTTGRLTSEMVI
                     KAAQMGVPIVVSRSGITQMGLQLARRLGLCAVGRALNRRFLCYSAPERLGLPVPPHGA
                     "
     gene            complement(19960..20856)
                     /locus_tag="PKN51_02725"
     CDS             complement(19960..20856)
                     /locus_tag="PKN51_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNT84042.1"
                     /translation="MPIFSTLRIDKDPAQPRIARLLLNRPERLNAINDDMPGEIRAAV
                     DWAVADDEVHVIVVEGAGKGFCGGYDLAATAEQHIEHPCQQESAPWDPALDYRAMKRF
                     TEDFMSLWRCPKPTIAKVHGAAVAGGSDIALCCDLLVMAEDARIGYMPTRVWGCPTTA
                     MWTYRLGPTRAKQLMFTGDVIDGRTAADWGLANQAVPAAELEAATLRLAARIAGVPRG
                     HLQMHKLVVNQVMLTMGLEQTQMLATVFDGITRHNPEGVWFRRHAQQHGFKSAVEWRD
                     SGRPIPEGAEAQALIRAMQGQG"
     gene            20934..23456
                     /locus_tag="PKN51_02730"
     CDS             20934..23456
                     /locus_tag="PKN51_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT84043.1"
                     /translation="MNLSSWTLGWRTLWRDLRAGELRLLMVAVTLAVAALSSVGFFAD
                     RLQGGLQRDARQLLGGDVVLVSDHAPAPGWTQRAAAEGLDSVLTLGFPTMGRAPEAQG
                     GATRLVALKAVERGYPLRGRLRLAQHAQDRAGTPTDAVPASGQVWVDPSLLEALALRR
                     GDTLLLGDSALRITELIALEPDRGTGFMSFAPRVLMNAADLPATGLVQPASRITWRLA
                     LAGPEPAVQRYRAWAEAELARPGMAGLRLETLDTGRPEMRTTLDRAAKFLNLVALLAA
                     LLSAVAVALAARAFAARHLDDCAMLRVLGLPQRRIAGAYVTEFGLVGLSASLAGVLIG
                     WLVHHIFVALLAGLVDSALPAPGPWPALLGLGIGMTLLVAFGLPPVLQLAQVPPLRVL
                     RRDVGALRPASLAVLGLGVAGFAALLLAVSADLTLGLIAVGGFAVAVALFALLSWVAV
                     RLLRRWVHEQRAPRWLVLATRQLSARPAYAVVQVSSLAVGLLALVLLVLLRTDLIASW
                     RAATPADAPNRFVINIMPEQGEAFRAVLRQAGVTRMDWYPMFRGRLVAINGQPVNPAD
                     YPEERARHLVDREFNLSHAAERPAHNDIAAGHWTTDEAGAISVEQGLAETLRLRLGDR
                     LRFDVAGMPLEARITSLRKVDWASMHVNFFAMFPRADMPDVPVTWIAAYRAPGQPGFD
                     HALVRQFPNITHVDMEQSIAQVQRVLDQVVRAVEFLFGFTLAAGLIVLFAAVSATREE
                     RAREFAVMRAVGAGSALLARVQQAELVGVGLLAGVLASAVAMGIGWAMARHAFDFHWT
                     ASPWVPLLGGVAGAGLALAAGWWGLREVLQRPVVQTLRRVAE"
     gene            complement(23578..23883)
                     /locus_tag="PKN51_02735"
     CDS             complement(23578..23883)
                     /locus_tag="PKN51_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84044.1"
                     /translation="MSARFFPLASTLAWTLIAGAASAAMPASGEGPFFNDPIRGPSDL
                     TRAEVMAMAIQSPPASGEGNGSQTTAPTPQPVDRETVVAELRDAMAQGFRPATGDIG"
     gene            24076..24990
                     /locus_tag="PKN51_02740"
     CDS             24076..24990
                     /locus_tag="PKN51_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT84045.1"
                     /translation="MLLRTFREVALRHNFSTAARTLDLAPATVSKYVAELEARFHLRL
                     FHRSTRRVTLTDAGQLLLDHCAQVIDLIDRTHDAMNERAQRPSGRLNLTAPHGLTRTI
                     LVRRLADFMLQHSDVSINLNLTNRMVDLVEDGVDVAFRVGAVADNSLIVRRLLPLEMV
                     VAATPAYWRAHGRPEHPRELATHRTLAMAPTGETPHWQFSIGGKLFDLPLHPVFCASD
                     SSPLVPLALQGVGVLRGSRMLLGEWIDDGRLEPLFAAYSPRNLWLYAAYAQRRHPSAA
                     LRALLDFLEQQMRPTVPSVPHAVSPRLH"
     gene            25112..25735
                     /locus_tag="PKN51_02745"
     CDS             25112..25735
                     /locus_tag="PKN51_02745"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNT84046.1"
                     /translation="MAQLFTVHPDNPQPRLLKQAAALLHGGGVLAVPTDSSYALVCHL
                     DDKAAVDRLRRIRQVDERHHLTLLCRDLSELASYARVDNTQFRRLKSGTPGPFTFILE
                     ASKEVPRRVSHPQRKTIGLRVPDHPALRELLALHGAPLLATTLILPGEGEPLNDAGAI
                     LEQLGHQIAGVIDAGACPLAPTTVVDLSGDAPEVLRRGRGDPARLGL"
     gene            25772..26440
                     /locus_tag="PKN51_02750"
     CDS             25772..26440
                     /locus_tag="PKN51_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNT84047.1"
                     /translation="MDFANIIQTLALYALPVLFAITVHEAAHGYAARHFGDGTAAFLG
                     RVTLNPARHIDPIGTIAMPLFLYIATGGAFVFGYAKPVPVNFGQLRHPKRDMVWVALA
                     GPASNLAMALAWGALLIVLDGVGVTEPFFLKMCQAGVLVNVVIGVFNLFPLPPLDGGR
                     ILVGLLPRGPALALSRIEPWGFFIVMALVVAGVISNLWMRPLMALSFHLLDFILAPLQ
                     ALFG"
     gene            26445..27758
                     /locus_tag="PKN51_02755"
     CDS             26445..27758
                     /locus_tag="PKN51_02755"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNT84048.1"
                     /translation="MASSVSRRVLTGITTTGTPHLGNYVGSIRAAVRASREPGVQSFY
                     FLADYHALIKCDEPARIQRSTLEIAASWLAAGLDPEQVTFYRQSDIPEIPELTWLLTC
                     VTGKGLLNRAHAYKAMLDKNAATGADPDSDVTAGLFMYPVLMAADILMFKAHQVPVGR
                     DQIQHIEMARDIAASFNHLYGELLTLPEALIDDNVALLPGLDGRKMSKSYDNTIALFG
                     PREQLRRQIMSIVTDSRAPGEPKDTEGSALYQIYRAFADDAEAAQFARAFADGIAWGE
                     AKQRLFERIDAEIGPMRERYQHLMSHPAEVEALLRRGADKARALATPLMRELRQAVGL
                     RALDTQVAAPRGRDSARTGLPSFKQYREQDGRFYFKLTGAGGQVLLQSRGFESPREAG
                     QAVKRLQSEGEHALGALAEWLEPVADALAPTLTEALQALQTAQQS"
     gene            27864..28469
                     /locus_tag="PKN51_02760"
     CDS             27864..28469
                     /locus_tag="PKN51_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT84049.1"
                     /translation="MAIYVIDDHPLMREAVVMLLRRIRPGSEVVELSQLSDLERAVET
                     RGAPELFCLDLKLPDTVGISGVKLLKRLYPDVPLAVISATPAADVEEWCTEAGADVYI
                     EKSAGASEIGAALRAMLQGDSEFADLEYGDSQTETKLSKRQKQLIVMLDEGLSNRDIA
                     ERLGISEHTVKVHLWRLFRRLGVKSRTQTLHFARTHGLLSS"
     gene            complement(28411..29826)
                     /locus_tag="PKN51_02765"
     CDS             complement(28411..29826)
                     /locus_tag="PKN51_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT84050.1"
                     /translation="MIPIPAPAPDPASRADWTEARQLAILMDGWPGTQLAVLALWALL
                     GGLLLLHAPGLRTGLWLGAALLVMAPGWRGWRSYRQRLAWAPLAEQRLRLRRQRASWL
                     VQALVWGMAPLCFAQRVAPEVEAMCWALTLGTGVLLVSWSSAHRRLAQGLASVLVAAL
                     LASLWLRLSGSPLRADLRGLWWQALLLLVYAAILWRIIGSQHQRVAEGIELSLRNARL
                     IESLREQTRVAQEAARFRTRFLAGAAHDLKQPVNAVGLYADWLSNEPELVDELAPKIL
                     QSTLAIHSLFDSLFDWVRLDAGHYELREREVAIRPLLSDLEVQFGPEARLKGLRLRVR
                     PADVVLRTDPVMLRRVIGNLLANAIRYTARGGVLLAARARPDGVSLEVWDTGIGIAQS
                     ELEQIFGEFYKVPTSGTEDGFGLGLSIVQALAERLGYRVTVRSRPGRGTVFRVWVPAP
                     AGLRNSAGRACERSAGSGSGS"
     gene            complement(29891..30955)
                     /gene="aroC"
                     /locus_tag="PKN51_02770"
     CDS             complement(29891..30955)
                     /gene="aroC"
                     /locus_tag="PKN51_02770"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNT84051.1"
                     /translation="MSGNTLGTLFAVTNFGESHGPAIGCVIDGCPPGLALSEADIQPE
                     LDRRRPGSSKFVTQRQEPDQVEILSGVYQGHTTGTPICLLIRNQDQRSKDYGDIAQSF
                     RPGHADYTYWKKYGLRDPRGGGRSSARLTAPTVAAGAVARKWLREQCGTSFAGCMTHL
                     GELAIGFEDWAHVPRNPFFAPVADVSALEDHMNALRKAGDSCGARLRVVARGVPVGLG
                     QPIYDRLDADIAKAMMGLNAVKGVEIGDGFASVAHRGSQHGDSPLPGGGFASNHDGGV
                     LGGISNGQDIEVAVAIKPTSSILVPRASVNLADEPVEVITKGRHDPCVGIRATPILEA
                     LLALVVMDHALRQRAQCGRL"
     gene            complement(31089..31556)
                     /locus_tag="PKN51_02775"
     CDS             complement(31089..31556)
                     /locus_tag="PKN51_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNT84052.1"
                     /translation="MRVRDILRLKGNALYTITPEDSLADAAQAMADRDIGSLVVMSHE
                     RVVGMLTFREVIQATVKNGGSLAGLSVYRSMDPGPLICTLETELDEVRRMMLEHHTRY
                     LPVIDNRQLMGVISFYDVAKAVVDAQDFENQMLKAYIRDWPEEDAAPAATERA"
     gene            31666..32976
                     /locus_tag="PKN51_02780"
     CDS             31666..32976
                     /locus_tag="PKN51_02780"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HNT84053.1"
                     /translation="MPGYADPGFDTLAVHAGARPDPATGARAVPIHLSTSFVFDSSDH
                     AAQLFNMERAGHVYSRISNPTNAVLEQRVAALEGGIGAIATASGQAALHLAIVTLMGT
                     GGHIVASSALYGGSQNLLHYTLRRFGIETSFVRPGDLDGWRAAIRPNTRLLLGETLGN
                     PGLEVLDIPAVAQIAHAAGVPLLVDSTFTTPYLLKPLEHGADLVYHSATKFLSGHGTV
                     IGGVLVDGGSFDWDRSGRFPELTTPYEGFHGMVFAEESTVGAFLLRARREGLRDFGAA
                     LSPHSAWLILQGIETLPLRMERHIANTEQVVRFLAEHPFVARVGHPLLASHPSHALAE
                     RLLRHGARGAGSVFSFDLKGSRAQGRVFIDTLKLFSHLANVGDCRSLVIHPASTTHFR
                     MNDEALAAAGIGPGTIRLSIGLEDPQDLIDDLKRALKAAEKAGA"
     gene            32981..33790
                     /locus_tag="PKN51_02785"
     CDS             32981..33790
                     /locus_tag="PKN51_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT84054.1"
                     /translation="MFIDLHGAPLYAYTGGRPFDPAQPTVVFIHGVLNDHSVWILQSR
                     ALAHHGWNVLAIDLPGHGRSGGQPPATVEQAADDVLALLRAAGIARAVLVGHSFGSLI
                     ALEAAARAPSQVSHLVLVGTAFPMAVSPALLEASVSAPEKAIHMVNVFSHSTLCPPPS
                     ALGPGTWLYGSSRALMRRVLASNPTVNLFHTGFQACDRYRGGEQAMAAVQCPTLLLVG
                     RHDQMTPPRAARALAAHARQATVVEVEAGHALMTEAPDAVLDALRQFLSRT"
     gene            complement(33895..34290)
                     /locus_tag="PKN51_02790"
     CDS             complement(33895..34290)
                     /locus_tag="PKN51_02790"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HNT84055.1"
                     /translation="MSPARLPSSPRLPLSRGVSITLQPGQARTLHLPAAGTLRVAQGA
                     LWVTRRGRHVDHPAQDLYVSAGLALALDAGDSVVIEPMHLAATPAAQVPPASFNWQAD
                     EGHCAGLLRWLRQHLQAAWRPAPARAPCA"
     gene            34410..35360
                     /locus_tag="PKN51_02795"
     CDS             34410..35360
                     /locus_tag="PKN51_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNT84056.1"
                     /translation="MLHAPTHLRSRPIQVGHLRAFEAVARHLNFRLAAQELALTQPAV
                     SRQIQALEDDVGVPLFRRHTRAVELTEAGNQLLTAVGQALPRLDATVRHIRQSAGRRH
                     VSVTTFASFASMWLIPRLEAFQRDHPDIDIRVEASDTAVDLEVTDIDVAVRYAPAERM
                     PAPAQRLFGESVTPMASPWLLRSGPPLKRPEDLLRHTLIEAGDAYTPHLQWLTWRRWL
                     DANGLKDAQPKRWLSFNYAYQMVQAALSGQGVVLARPPLVAENLARGDLVEVFAAGAY
                     PQARLISPMAYWVLVSPHSRQRAEVRAFADWLLMQAQSTR"
BASE COUNT         5277 a        12177 c        12442 g         5704 t
ORIGIN      
        1 cgtcgagcgc gagggcgagc ggattttcga gcatgattga cgcacgtcaa tcaaaaccac
       61 aatagcttcg cggacactgc gtagggtttt tcaagtaaga ggtggaaatg acattcgcca
      121 acacacaggc tactgtctat aacgacaaag tggtcaggca attcgccatc atgacgatcg
      181 tctggggcgt ggtggggatg ctggtcggcg tgatcatcgc cgcgcaactg gcatggcctg
      241 aactgaactt cggcattcca tggctcagct atggccgctt gcgaccgctg cacaccaacg
      301 cggtgatctt cgccttcggc ggcagcggcc tcatcggaac ctcgtactac gtggtgcagc
      361 gcacctgcca ggtgcggctg ttctccgaca agctggcggc attcacgttc tggggctggc
      421 agctggtcat cgtcgccgcg gccatttccc tgccgctcgg ctacaccacc agcaaggagt
      481 acgccgagct ggagtggccg atcgacgtgc tcatcaccct ggtctgggtg gcctacgcgg
      541 tggtgttctt cggcaccgtc ggcacgcgcc gcgtgcgcca catctatgtg gccaactggt
      601 tctacggcgg cttcattctg gccattgcgc tgctgcatgt ggtcaacagc gccgagctgc
      661 cggtcggact gatgaagtcc tactcggcct acgccggggt gcaggatgcg atggtgcagt
      721 ggtggtatgg ccacaacgcc gtgggcttct tcctgaccgc tggtttcctg gggatgatgt
      781 actacttcat tcccaagcag gccgagcgcc cggtctacag ctatcggctg tccatcgtgc
      841 acttctgggc gctgatcttc acctacatgt gggccggtcc gcaccacctg cactacaccg
      901 cgctgcccga ctgggcgcag tcggtgggca tgatcttctc tctgatcctg ctggcgccca
      961 gctggggcgg catgatcaac ggcatcatga ccctctcggg cgcctggcac aaactgcgtg
     1021 atgacccgat cctgcgcttc ctgatcgtct cgctgtcgtt ctacggcatg tcgaccttcg
     1081 aggggccgat gatgtcgatc aagaccgtca acgccctctc gcactacacc gactggaccg
     1141 tgggccacgt ccactctggt gccctgggct gggtcggcct gatcaccatg ggctcgatgt
     1201 actacctgat cccgcgtctg ttcgggcaga agcagatgca cagcgtcaag gccatcgaag
     1261 tccacttctg ggtcgccacc gtgggcatcg tgctctacat cgccgcgatg tggattgccg
     1321 gtgtgatgca gggcctgatg tggcgcgccg tcaaccccga cggcaccctg acctacacct
     1381 tcgtcgaatc ggtgaaggcg acctacccct tctacgtgct gcgtctgatg ggtggcctgc
     1441 tgtatctggg tggcatgctc atcatgctgt ggaacaccat caagacggcc acggcgggcc
     1501 gtgtggtgcc ggtgcagatc cctgcggttg ccgcgcacgc ctgaggagga gttgagcagt
     1561 catggcacaa gaaaaagtca ccggccacgc caaggtcgaa accagcaact tcctgatgat
     1621 cgtgctgatt cttctggtgg tgtccatcgg cggattggtc gaaatcgttc cgctgttctt
     1681 ccagaagtcc accacccagc cgatcgaggg gctcaagccc tacaccgccc tgcaggtggt
     1741 cggccgcgac gtctatgtgc gcgagggttg ctacaactgc cactcgcaga tgatccggcc
     1801 gttccgcgcc gaggcactgc gctacgggcc gtactcggtg gccggcgagt tcgtctacga
     1861 ccacccgttc cagtggggca gcaagcgcac cggccccgac ctggcccgcg tgggtggacg
     1921 ctacagcgac gactggcacc gcgcccacct gaacaacccg cgtgacgtgg tgcccgaatc
     1981 gaacatgcct gcctatccgt ggctggagcg caccgccgcc aacgccggca cgatccaggc
     2041 ccacatgcgt ggtctgcgca ccctgggcgc accgtacacg gatgaggaaa tcgccaaggc
     2101 gccggaagag gtcaagggca agacggagat ggatgccgtg attgcctacc tgcaggtgct
     2161 gggcatccat cgcaaataag cagcatggga gcctccgtca tggacatcaa cctgttgcgc
     2221 agcattgtca ctgtgctgtc cttcgtgctc ttcatcggca tcgtggtctg ggcctggtcc
     2281 gcacgcaacc gagagcgctt cgacgaagcg gcgcaactgc cattcgagca gcaggattga
     2341 ccctgagcaa cccgacaggc aaggaattca aaaatgagcg actttacgag caatttctgg
     2401 tcggtgtacg tggcgggaat cacgatcgcc ggcatcatcg gctgcctgct gctgctgtgg
     2461 gtcagtggca agaccaaggc catgaccgac agcgacaaca ccaccgggca tgtgtgggat
     2521 caggatctgc gggagatgaa caacccgctg ccgcgctggt gggtgggcct gttcatcatc
     2581 acctgcgtgt tttcgctggc gtacctgttc ctgtacccgg gtctgggcac ctaccagggt
     2641 tccctgaact ggtcgcagac cgatcagttc gagcgtgagg tggcgcgtgg caacgagcag
     2701 gtggcgccca tctatgccgc gtttgccggc aagagcgcag ccgagctggc caaggatccg
     2761 caggccatgg cgatcggcga tcgcctgttc atgaacaact gcgcccagtg ccacggctcg
     2821 gacgcgcgcg gctccaccgg ttttcccaac ctgaccgacg gtgactggct gtggggcggc
     2881 aagccggagc agattcacga atccatcacc aaggggcgca ccggcgtgat gaccccgatg
     2941 ggcgcggccg tgggcagtgg ggacgacgtg cgcaatctgg ccaactacgt gctcagcctg
     3001 tcgggcggtc cgcacgattc ggtgcgcgcc aacctgggca agcccaagtt tgcggtctgt
     3061 gcggcctgcc acggggccga tggcaagggc aacccgctgt tgggtgctcc caacctgacc
     3121 gacaacatct gggtgcatgg tccgggcgtg gagtcgcatg tcgtcaacat gatcaacaac
     3181 ggcaagaccg gcgtcatgcc tgcgtgggaa agcaagttca cacccgagca actggcggtg
     3241 ttgacggcct atgtctgggg caagggcggc ggtgtggcgg atgccgccgc ggccgccacc
     3301 tcgaaggcgg gcgcagccgc cgccgatgcg gcctccgtgg tggtcgaagg tggcgtggtc
     3361 aagttcttct ttgccaccgg caaggccgat ctggcgcccg aggccgacaa ggcgctggcc
     3421 gacatcctgg aaggggtgaa ggcaggcaag aaggccgtga tcagcggctt cgtcgattcc
     3481 accggcaatg ccgagcagaa tgccgaactg gccaagcagc gggcgatcgc ggtgcgtgac
     3541 cagctcaagg cgctgggggt ggccgaagac caggtggagc tgaagaagcc ggccaacatc
     3601 gatgccggcg ccgacgccca ggcgcgtcgc gtcgaagtct ccctcggctg aggtgaaacc
     3661 gtgcggcggg cgatggaaat cttcctcgcg gccgcctccg ggggcgggta gacccggctt
     3721 ggtgaccaca tcacaatcac aacccgcagg agacagagtg gattcggggg gcgcatggcg
     3781 ccccgttttc cttggtcagg tggttgacgt cggcgccgcc tggatcatcg cccgttcagg
     3841 gtccgaatcc tgcatgggcc gaccactgac ggacaggcag gcgtgagcaa tccaacccca
     3901 tctccgaaga aggtcgcggc ggcggcgggc gaagacacca tggtgtcgct gtatcaggcg
     3961 cacaagaagg tgtatccccg cagcgtcacc ggactgtttg ccaagtggcg ctggttcttc
     4021 gtttttgcga ctcagatttt cttctacggc atgccgtggc tccagtgggg cgagcggcag
     4081 gccatgttgt tcgatctgga ggcgcgacgc ttctacatct tcgggctggt gctctacccg
     4141 caggacgtga tctacctgac cgcgatcctg atcatttcgg cgctgtcgct gtttctgttc
     4201 acggcggtgg ccggacgcca gtggtgtggc tacgcctgtc cgcagactgt ctacaccgag
     4261 atctacctgt ggttcgagcg catgatcgag ggcgaccgtt cagcgcgcat gcggctggac
     4321 gcggcgccga tgtcggccgc caagttcggg cgcaaggcgc tcaagcaggc ggtgtggatc
     4381 gctttcggcc tgtggacggg ctttaccttc gtcggctact tcacgcccat ccgcgagttg
     4441 gcgcagcatg tgctgcagtg gaacctgggc gcatgggaga tcttctggat cttcttctac
     4501 ggctttgcca cctatggcaa cgccggcttc atgcgcgagc aggtgtgcat gtacatgtgc
     4561 ccgtacgcgc gcttccagag cgcgatgttc gacaaggaca ccctgatcgt cacctacgac
     4621 gccaagcgtg gcgagccgcg cggcgggcgc tccaagagcg ccgatcccaa ggcgctggga
     4681 ttgggtgact gcgtcaactg cacgttgtgt gtgcaggttt gtccgaccgg gatcgacatc
     4741 cgaaacggtc tgcagtatga gtgcatcagt tgcgccgcct gcatcgacgt ctgtgacgag
     4801 gtgatggaca aggtcggcta cccgcgtggc ctgatccgct actccaccga gcatgcactg
     4861 gaacagggct ggggccgcaa ggagatcctg cgccaggtgc tgcgtccgcg cgtgctgatc
     4921 tacgtggcca tcctgtctgc ggtggtggtc gcgctgatcg ccagtctggt gctgcgcaca
     4981 ccgttcaagg tggacatcgt gcgcgaccgc gccaccctgg cgcgcctggt cgatggcaac
     5041 cagatcgaaa acgtgtaccg catccaggtg atgaacgcga ccgaaaagcc gcagaccttc
     5101 cagctttcgg tggaagacct gccgggtctg gaaatcgcct ccgacaagag cgtgaccgtc
     5161 gacggcgcgc agagcctgtg ggtgcctgtg cagctgcgac tgccctacgg cagcgccagc
     5221 agcggctctc acaccattca tttccgcgtg accgcgcccg gcgtcggcga agtgcgcgaa
     5281 aagtccatct tcatggtgcc gaggtgatga ccatgtccga ccctgcttct accccaccca
     5341 gcccctggtg gaaattcggc cacatgtggc tggtcatcgc cgggccgctg atcgtggtgc
     5401 tggcctccat cgtcacgatc tatctggcgg tccgcacgcc cgacccggtg tatggggatg
     5461 cgtccagggg tgccgcccga tcgtcgcaag ccaaggatgg cggcgacgcc ggccgcatgt
     5521 cgcccgccat gcaggcgcgc aaccatgcgg ccaccggggg cgtcgccaga ccagcgccga
     5581 accacggcac cggcccgaac cagtgataat gatccaggtc aaattcaaag gggacaaacc
     5641 gcgatgctgc gagagcgact gatgtggatt gcgtggccag cgtttctgat ggcggccgtg
     5701 ctggagttca tggtgtttgc ggtgctcgac ccgcaggcgc tgacgctgtt tggcgaaccc
     5761 tcgggctggt cgcgcgaagg cgtctacacc gtcacgttct tcattttctg ggcggtgatg
     5821 atgctgtccg gcgccctcac caccctgctg tcgaagtcgc ccttcgacgt caatcgccgc
     5881 cagtcacgcg ccctgcgctg atcgcgcggt gcggccgatc gggtcgggcg cccggggcgc
     5941 cagcccatcc cacgcgaatc gacggctggg ctggaacagg aatggggggt gacgagcctt
     6001 gaccccggca ctgactacaa agttagggct gcttggttcc ccacctgacc cggttggctc
     6061 cttcaccatg cgggaaggcc cgtcgctggc tattgtacgg cttgcagacg gcgttcggct
     6121 tcggccaagc cgaaactcat ggccaccagg ttcagcacct cggcgcgcaa ctggccaagc
     6181 tgttcatcgt ccgggtcggc cgccagccgt gccttcaggc gcgctgcgcg ggggtggatg
     6241 cagtctgtca gggcgccgac cagggccgcg atgcgcggat cggcgtgggt ctgaatgacg
     6301 atcgtgtcgt ggctctcgaa aacgtccatc atggcgtaca gagtgaaccg tggcggggca
     6361 agccatcatg gagcaagcgc ttgctgcatt gcagcatatc cggtttctgg cccgtcgggt
     6421 gcattccgtt gcatttgtgc gtcggtattc gaggcgggtc tctaattcgg gccatgcttt
     6481 tcccgccacg tagaatcgcc gcatgtcgta tctggtgctg gcccgcaaat accggccgcg
     6541 gaatttctcc gagatggtgg ggcaggagca tgtcgtccag gccctcacca acgcgctgac
     6601 gcagcagcgc ctgcatcacg cctacctgtt caccggcacg cgcggcgtgg gcaagaccac
     6661 ggtgtcgcgc atcctggcca agtcgctcaa ctgcaccggc gccgacgggc agggcggcat
     6721 caccgccacg ccctgcggcg tgtgcccggc ctgtgccgac atcgacgccg gccgctttgt
     6781 cgactacacc gaactggacg ccgccagcaa ccgcggcgtc gacgaggtgc agcagctgct
     6841 ggagcaggcc gtctacaagc cggtgcaggg gcgcttcaag gtcttcatga tcgacgaagt
     6901 gcacatgctc accggccacg ccttcaacgc catgctcaag acgctggagg agccgcccga
     6961 atacctcaag ttcgtgctcg ccaccaccga cccgcagaag gtgccggtga cggtgctgtc
     7021 gcgctgcctg cagttcaacc tgcgccccat ggcgcccgag accgtgctgg accacctgac
     7081 gcgcgtgctg gcggccgagg cggtggcggc cgaaccggcc gcgctgcgcc tgctggcgcg
     7141 cgccgcacgt ggctccatgc gcgacgcgct cagcctgacc gaccaggcca ttgccttcgg
     7201 ttccggtgaa ctgcgcgaat ccgcggtgcg ccagatgctg ggcagcgtgg accgcagcgt
     7261 ggtgttcagc ctgatcggcg cgctggctga cggcgatggc cgcaccgtgg tgacgctcag
     7321 cgagcagctg cgcgccagtg gccactcggc ggcccagacg ctggaagaca tggccagcgt
     7381 gctgcagcgc atggcggtgc agcaggccgt gcccgatctt gcggtggacg cgtccgaccc
     7441 cgacgcgcag gaaaccgccc gcctggccgc gcgcctgccg gccgacgaaa cgcagctgct
     7501 gtacagcctg tgcatccacg gccggtccga gctgggcctg gcgccggacg aatacaccgg
     7561 tctgaccatg gcgctgctgc gcttgctgcc gtttcgccag ccggtgccga cccaggccca
     7621 gactgtcagc gccacggctg aaaaaaaaag cctgaaaaca cctgaagcct cgcccgcgcc
     7681 gcagggcgcc agcgctccgc ctgcgaaggc gcccgcgcca gcgccggtcg cggcgagcga
     7741 cgaccctgct gcggcagagc agggtgcggc agaactgggt gcggccgcag acgcccggcc
     7801 gcaaccggtc acggaacgcg acgccgaact ggacacgcag ggcgcgccac ccgcccgccc
     7861 ggtgcccgcg ccgcgcccgc agccggcgct gcagccgctg ccggtgcggc cgtcgaacgg
     7921 cacccgcttt caagaacaaa atcaacctcc agcgcccgac catattgcgc cagtagctcc
     7981 tgaaacaata gcaattcaag tgcgtgaagg agaccaggag ccggcgccgg cgccgcgcct
     8041 gcgggtcgac gccccgccgc cgccggtggt gcccggcgcc gaaggcgact tctggcacgc
     8101 gctggtgcag gagctggtgg cgcgcgaagc cgtgacggcc ctggtgcgcg agctggcctt
     8161 gcagtcgcag ctggtggcgc gcaccgatcg gcagtggacc ttgcgggtgg aaagcgagtc
     8221 gctggcccag tccggcgtgc gcgagcgcct gcaggcggcg ctggccgacg ccggccactg
     8281 cgtgcgcctg cagatcgact ccggcccggt cagcgacagc ccggcgcgtc gcaacgccat
     8341 ccacgccacg cgccggctga aggcggccga ggcgctgctg ctggccgacc cgttcgtgca
     8401 ggagatgatg cgcgactttg gggcgaaaat cgtgccgggc agcatcaaac cgctctgaat
     8461 gaggcgcgcc gcagacgaac ccacccgcgc ggtgcgcggc ggtgctcacc gccggccgat
     8521 ggcgccaccg ccgcctgggc cacagccccg gaacacgcat tgaagaaagg aagaaccgat
     8581 gttcaacaaa ggacaactcg ccggcctgat gaagcaggcc caggccatgc aggacaacct
     8641 gaagaaggcg caggatgagc tggcgctgat ggaggtggag ggcgaatccg gcgccggcct
     8701 ggtcaaggtg ctgatgacgg ccaaacacga cgtcaagcgc gtcaccatcg accccagcct
     8761 gctggccgac gacaaggaca tgctggaaga cctggtggcc gccgccttca acgccgccgt
     8821 gcgcaaggcc gaggagctgt cggccgagaa gatgggcaag ctgatgcccg ccggcatgcc
     8881 cgggttgccg gggggcatga agttcccgtt ctgatgacga atgacggcgc tggcgcgcca
     8941 tgaccttgcc gctccgcagc tgcgacgaca tggggcatgg gctcgaccgc cacggcggcg
     9001 ccgttgccgt catcgagccg caaccaggcg aggtcacttc gtcagggggc atgccagcat
     9061 gagcgacacc cacagcctcg acaccctgat ccaggccctg cggcgcctgc cgggcgtggg
     9121 ccagcgctcg gcgcagcgca tggcgtttca cctgctgcag cacgatcgcg aaggcgcgca
     9181 gcagctggcg cgggcgctgc acgaggcggt cggtcaggtg cgtcactgcg cccgctgcca
     9241 caccttcacc gaggccgagg tgtgcgccac ctgcagcgac ccgcggcgcg acgccagcca
     9301 gctggcggtg gtcgagaccc cggccgacca ggcggcgctg gagcgcaccg gggccttccg
     9361 cgggcgctac ttcgtgctga tgggccggct cagcccgctg gacggcatcg gcccgcgcga
     9421 catcggcgtg cagcggctga tcgagcgcgc ctgcgacggc gtggtgcgcg aagtgatcct
     9481 ggccaccaac ttcaccgccg agggcgaggc cacggcgcac ctgatcgggc aggcgctgcg
     9541 cagccgcggc ctggccgtga cgcggctggc gcgcggcgtg ccggccggca gcgagctcga
     9601 atacgtggac ctgggcacga tcgcccacgc gctggtcgac cggcgctgaa ccgccatgtc
     9661 gctgtccgcg tcgatcctgg ctgcccgcca gctgctgcag gaggcggcgc acatcgccgt
     9721 gctcagtggc gccggcatca gcgccgagtc cggtgtgccc accttccgcg acgcccagac
     9781 cggcctgtgg gcgcacttgc gcccggagga tctggccacc gaagacgcct ttcggcgcca
     9841 ccctcagcgc gtgtgggact ggtacgccga gcgccgcgcc agcctggccg aggtgcaacc
     9901 caacgccggc caccgggcgc tggccgaatt ccagcaccgc cacccgggtc ggctgacggt
     9961 gatcacgcag aacgtcgacg gcctgcacca gcgcgccggc agcaccggcg tgctggcgct
    10021 gcatggcagc ctggcggccg accgctggct cgatccctgc cctgcggggc gcggcccgac
    10081 cgcctgcgat gtcgcgcggg ccgaacctgg ccgccccccg cgctgcgccc agtgtggcaa
    10141 ctgggtgcgc ccggcggtgg tctggttcgg cgagctgctg cccgaagacc agctgcggca
    10201 ggccgaacag gccgccagcc gctgcgacgc catgctggtg gtcggcaccg ccggcgcggt
    10261 gtatccggcg gccgggctgg cgcaccaggc gcgtgccgcc ggggcgcgtc tgatcgtcgt
    10321 caatccccac cccagcgagc tggacgggct ggcccagctg cggctggtcg gcggcgcggc
    10381 ccaggtgctg ccgcccttgc tggacttttg accacgcggc cgctgcggca gcgccctgcg
    10441 gtgccgcagg tgcctcgtga attgctattg atgcggtagc tgctggcgca tgcctggtgc
    10501 tgcctcaggc cggatttgat gctccatcgg cgcggcccgc ccggggccgc gcgctcaggt
    10561 gctggcccac atgcgcagca ggttgtggta ggtgccggtc aggcggatcg cctcgtcggt
    10621 ttcgccgtgg cgctggcgca gcgccagcag cgccatgtcc atctcgaaca gcaggcggcg
    10681 ctgctcctgg ccgcgcacca tggattcgat ccacagaaag ctggcgatgc gcgcgccccg
    10741 ggtgaccggg cgcacctcgt gcacgctggt gccggggtag agcacggcgt cgcccgccgg
    10801 cagcttgacg gcctgcgggc cgaaggtgtc gtgcaccacc agctcgccgc cgtcgtagtc
    10861 gtcgggctcg gtcaggaaca cggtgcacga aaggtcgctg cgcacccatt cgccgctggt
    10921 ctgtgagtgc agcacggcgc catccacatg ctggccatag tggttggccg caccttcgta
    10981 gcggttgaac agcgggttga agatcctgcg cggcagcgcc gccgagaaga acaccgggtc
    11041 gcgccgcagc gccgccagca ccagctcgcg cagcgtggcc gcgctggggc tgtcctgcgc
    11101 cagctggcgg ttgctcttgc gctgcacggc ctggccgccg gcgctgcgcg cgccatcgac
    11161 ccagggtgcg tcgtcggcca gcagcagacg gcgcgcgtgc gccacttcgt cggcgctgag
    11221 caggttcttg aggtgaatca acatgggaga ttccgccaaa gacgacagcc gcccgagggc
    11281 ggctgtgcag gttggatgtg gtggtcagtc catcagaaca ggtgcttgag cgtcagctcc
    11341 acccggcgtg gcgcgcccgg ttggtagaag ccccggtaca gcccgtcggc gtacagcttg
    11401 ttgctcaggt tggtgacgct gagcttgagc gtggtggctt cggtgaagct gtattcggcc
    11461 atggcgtcga aggtgacgaa gcccttggcc ttcaaagtgc gctggccttc cgggttctgg
    11521 ctgctgcggt agttggcgcc cagccccagg cgcagttgcg gcatcacgcg gtaggtggtc
    11581 cagacgctgc cgctgtggcg cggcgtcagg cccgggcggt cgccctgcac ctgcgcgccg
    11641 gtgccggcgg ggttcagcac cacgttgctc ttgtcgatct tggctttcgg gatccaggtg
    11701 tggttccaga acagttccca ggccggcgtg atgcggcccg ccaggttgaa ttccatgccg
    11761 gcggcgtggc gcttgccgga cagcagctcc tgcgtggcgg cggtgtcggg gtcggtgttg
    11821 cgctcgttgt acttctcgct gtagaacagg gcggcgccgg ccagcatgcg cttgtcgaac
    11881 aggtcccact tgccgccgat ttcgatgttg cggctctttt cgggcggggt cttggccgag
    11941 cggccggtgt tggggttgat cagcacgccg tactgatagg cgtcgcccga ggtgttgaac
    12001 gaggtgccgt aggagatgta gtaggacgac gcctcgtcgg gctgatagag cagacccagg
    12061 cgcgggctcc acaggttgtc ggtgcgcgcg ttgctgagcg agccgtcggc gttgcggtac
    12121 gagcccttga agtggtcgaa gcgcaggccg ccgaccagct tgacggtgtc ggtgagcgag
    12181 acggtgtcct gcaggtaggc gccgatgctg cgcgagtcga agctgttgta gggcaccggc
    12241 gtgacgcggg tgtcggtcac ccaggcgccg tcgttgggcg tgcccacggt ggtgttgggg
    12301 cgcgccgggg tgttgggcag cgggtagttg ttgttgcgct tggcgctttc gtcgtagacg
    12361 tcgatgccgg tgatcaggct gtgcttgcgt ccgcccagct cgaagctgtg gctgtagtcg
    12421 ctttgcagca tcaccacgtc gcttttgccg atgcggccct tggagctgcg ggtgagcacg
    12481 gtggatgcgg tgatgtcgct cagcgcggtc gggcgcggct gggcaaaacc gatcgggctg
    12541 cccagcaggt cgcgctcgta gcggccggca cgcaggcggg tcttgagctc tccgccgtgg
    12601 tcgaagcggt gcgtgtggcc cagcgtggcg taggtggcgc tggtgtccag atggtcgttc
    12661 ttcaggccgt agaagttctt ggccggcagc gtggggtgga tggtgccgtc ggcgccgatg
    12721 atccagggat gggcgtagtt cggccggccg tcgatgtcca ggtggtagaa gccgaccgtg
    12781 aattcgtcgc gcgtgccgat gccccagcgg aaggtgggcg ccacgccgat cttgcgctgc
    12841 ctggcgcccc agttgctggc gtcgtgggcc atggcgttga gccggaaggc cgagtcctgg
    12901 ccggtctgcc agttgaagtc gccctgcacc cggcccatgc ggccggtgcc ggcggtcacg
    12961 ctggcttcgt gctggttcat caggaagggc tgtttgctga cctggttgac cacgccgccg
    13021 gtcgaaccct tgccgaacag catcgaggcc gagcccttga tgacttcgac ccgatcgtcg
    13081 ttgaaggtgt cgcgctcgta cagcggcgcg tcgcgcatgc cgtcgcggta gatgtcgccg
    13141 gcctggccga gcgagaagcc gcgcaggcgc acgtcttcct cgccggtttc gccggcctgg
    13201 aaggtgacgc cggcggtggc gcgcagcacg tcgcggaagt cgtccaggtt gcggtcctgg
    13261 atcagccgct cggtcatcac cgtgacgctt tgcggaatgt cgcgcagctc ctgattgccc
    13321 ttgccgatct cggtgcgggt ggcgcgcagc tgtgacttgc tggtggtggc ggcgccatcg
    13381 gtggtggcgc tgtcgcgcac ggtgacggtt cccagggtgc cggccaccgg ggcgctggcg
    13441 ggcgtctggg ccagcgcggc ggtgctcagc gagccggcca gcagcatcgc ccccaggggc
    13501 agcagcgggg tgctgtcggc ttcaaaaaca ggagcattcg gcgcttgctg gatcagagat
    13561 tctggctttt ttgctttgga tttcgaacgg atgggcggca tggggtgctc agcagaaaac
    13621 gaaaacggag aagtgctggc tacctgggcc ggggcggaaa gacgggaggg tggctttccg
    13681 aggccgttcg ctggcttgca atgaagtcgt tgatcttaag caaaatgata atcattgtca
    13741 ttcaatttgt tgcagatcaa cgacgggcac gaaaaagggg ccttgcggcc cctggctgtg
    13801 cggtcggtcg cggcgcgcct cagcttggcg cacccatctg gctttgcagg tagttctgca
    13861 ggccgacttt gtccagcagc tcgatctggg tttccagaaa gtcgatgtgc tcttcggtgt
    13921 cgtccaggat gtcctgcagc agatcgcgcg agacgtagtc gcgcacgctc tcgcaatggg
    13981 cgatgccgtc cttgatggtg gcctgcgccg cttgctcgag cttgaggtcg cagcgcagga
    14041 tttcgggcac gtcctcgccc accatcagct tgcccaggtc ctgcaagttg ggcaggccat
    14101 cgagcatcag gatgcggtcc atcaggcggt cggcgtgctt catttcgccg atggattctt
    14161 catattcctt cctggccagg cgctccaggc cccagtgctt gagcatgcgg tggtgcagga
    14221 agtactggtt gatggcggtc agctcgttct tcagctgagc ctgcaggtgg gcaatgactt
    14281 tggcgtcgcc ttgcatggcg ttctccttgt gggtggcgat ggcccagcca ttctaggagc
    14341 gcgttcacct gcgttcagtg cagggatttt cggaatgaga ccggttcgcg tgcggagcgc
    14401 gaccgcaggg cgttgggctc gggctttcct cgggctttcg ccatgcgacg cggcgctgca
    14461 caatgcccgc agccatgacc gacacccgcc ctgccgccca gccccagcct gaacctcagc
    14521 gtcggcgtgc gcgcgaccgg ctcaagggcc gccagcccga ggccggtgcg cgcgacgagg
    14581 tgcgcgccct gatcggcacg cccggccccg acggccaccg gcgcgacctg ctgatcgagc
    14641 acctgcaccg gctgaacgat cgctaccacg gcctgttcga gcgccacctg gtggcgctgg
    14701 cggccgaaat gcgcctgtcg atggccgagg tgtacgaggt cgccagcttc taccatcact
    14761 tcgaagtgcg caaggatggc gaggccgcac cacgtctgac ggtgcgggtg tgcacatcgc
    14821 tgagctgcca gctggccggt gccgatgcct tgctggcgcg cacgcgcgag ctgctgggcg
    14881 ccgaggtgca ggtgctggcc gccccctgca tcggccgctg cgagcaggcg ccggccgcgc
    14941 tggtgggacc gcatggcgtg gggcaggcca gcgccgagcg gctggttcag gcatcaaatc
    15001 gggctctggc gcttgatggt cctgcgccaa cagctataaa accaatagtg tttgacgcct
    15061 acacgcaagc tggcggctac accctggcgc aggccgtggc gcgcggcgag ccgggccacg
    15121 aggccatcat cgccgcgctc gagcacgccg gcctgcgcgg cctgggcggc gccggctttc
    15181 ccgccgggcg caagtggcgc atcgtgcgcg acttggcccg ctccgccgag cgccgggccg
    15241 ccccaagcga ggcggcggcc ccctcggggg gcagcggacc acgcgcagcg ggcgagcgtg
    15301 ggggcatgac atatctggcg atcaacattg atgaaggcga gcccggcacc ttcaaggacc
    15361 gctggtacct ggagcgcgac ccgcaccgct ttctggaagg tctgctgatc gccgcgcagg
    15421 tggtgggggt gcagggcgtg tacatctacc tgcgcgacga ataccccgaa tgccgcacca
    15481 tcctgacgca ggcgctggcc gacctgcgcg ctgcgcccgg gctgcgcgag ctgctgccgc
    15541 cgaccgagct gcggcgcggc gcgggcgcct atatctgcgg cgaagaatcg gcgatgctgg
    15601 agagcatcga gggcaagcgc ggccagccgc gcctgcgccc gccctacatc gcccaggtgg
    15661 ggctgtttgg ccggcccacg ctggagcaca acttcgagac cctgtactgg ctgcgcgaca
    15721 tcctgcagcg cggccccgac tggtttgccg cgcaggggcg ccacggccgc cagggcatgc
    15781 gttcgttcag cgtcagcggg cgggtgcggc agcccggcgt caagtgggcg ccggccggca
    15841 tcacgctgcg cgagctggtg gacgaatact gcggcggcat gctggacggg catgagctgt
    15901 acgccttcct gcccggcggc gcctcgggcg gcatctttcc ggcccggctg gccgacgtgc
    15961 cgctggactt cgacacgctg cagccgcacg gcggcttcat cggcagcgcg ggcgtgatcg
    16021 tgctcagcca gcacgacaag gcgcgtgacg cggcgctgaa catgatgcgg ttttttgccg
    16081 ccgagagctg cggccagtgc acgccctgcc gggtgggcac tgccaaggcg gcccgcttga
    16141 tggaagcgcc ggtgtgggac gcggccaccc tgactgatct gagccaggtg atgcaggacg
    16201 ccagcatctg cggcctgggc caggccgcgc ccaaccccat gcagtgcgtg caacgctact
    16261 ttgcgcacga ggtggaggca tgacgccggc actcgcttgc attgctttcg aactcgacgg
    16321 ccagcccgcc accgccttcg agggcgaaac catcctgaag gccgccgagc gccacggcgt
    16381 gcaaatcccg cgcctgtgct tcacggacgg cctgcgcgcc gacggcaact gccgcgcctg
    16441 cgtggtcgag gtggacggcg agcgcacgct ggcgcccagt tgctgccgcg ccgtgcagcc
    16501 gggcatgaag gtgcgcgccg ccagcgagcg agcgcgccgc gcccaggata tggtgctgga
    16561 gctgctgctg gccgagctgc ccgcggccgg tttcaagtgg ctggacgatg acgccacgca
    16621 gccccatggc gaactgagcc agtgggccag ccagcgcaag gtgggtgtgc gccagcagct
    16681 acaaaatttg cagcgcccac ggcccacccc cgacctcagc caccccgcca tggcggtcaa
    16741 cctggacgcc tgcatccagt gcacgcgctg cgtgcgcgcc tgccgcgagg cgcaggtcaa
    16801 cgacgtgatc ggcctggccg gccgcggcgc cgaggcgcgc atcgtgttcg acctggacga
    16861 cgccatgggc gccagcagct gcgtggcctg cggcgaatgc gtgcaggcct gccccaccgg
    16921 cgcgctcatg cccaagaccc acatcggccc gcagcgggtg gaccgcagcg tcgattcggt
    16981 ctgcccgttc tgcggcgtcg gctgccagat cacctaccac gtgcgcgatg aaaagatcgt
    17041 cagcgtgagc ggccgcaatg ggcccgccaa cgccggaagg ctgtgcgtca agggccgctt
    17101 cggctttgac tacgccgcca gccccgaccg gctgacgcgc ccgctgatcc gcatcgccgg
    17161 cgtgcccaag gacccggcgc actgtcagca gccgcccgac tggcgcacgg tgtttcgcga
    17221 agccagctgg gacgaggcgc tggacgcggc cgccggcggg ctgcgcgcgc tcaagtcgcg
    17281 gcacggcccc agggcgctgg caggcttcgg cagcgccaag ggcacgaacg aagaggccta
    17341 tctgttccag aagctggtgc gcaccggctt tggcagcaac aacgtcgacc actgcacccg
    17401 gctgtgccac gccagcagcg tggcggcgct gctggagggc gtgggctcgg gcgcggtcag
    17461 caacccggtg cgcgatgtcg aacacgccga gctgatcctc gtcatcggcg ccaatcccac
    17521 cgccaaccac ccggtggccg ccagctggat gaagaacgcc gcccggcgcg gcacccgcat
    17581 cgtgctggcc gacccgcgcg ccaccgacat cgcgcgccac tgctggcgcg tgctgcagtt
    17641 tcgccccggc agcgacgtgg cgctgctcaa cgccctgctg cacgtcatcg ttaccgaggg
    17701 gttgggcaac gaatcgttcc tgcgccagcg cgccgaccac ttcgccgcgc tggccgccaa
    17761 cgtgcagggc tacagccccg aggccatggc gccgatctgc ggcatcccgg ccgaaaccct
    17821 gcgcgaggtg gcgcgcgcct atgccacggc gcgctcggcc atgatcctgt ggggcatggg
    17881 cgtgagccag cacgtgcacg gtaccgacaa cgcgcgctgc ctgatcgcgc tggccacctg
    17941 caccggccag ctcgggcgcc ccggcaccgg cctgcacccg ctgcgcgggc agaacaacgt
    18001 gcagggcgcc agcgacgccg gcctgatccc catgatgtac cccaactacc agcgcgtgga
    18061 cagccccgat gcgcgcacct ggttcgagga cttctggggc cagccgctgg accccgaacc
    18121 cggctacacc gtggtcgaaa tcatgcgcca ggcgctggcc gccgacgacg acccgcacaa
    18181 ggtgcgcggc atgtacatca tgggcgaaaa cccggccatg agcgaccccg acctgaacca
    18241 cacgcgccac gccctggcca gcctgtcgca cctggtggtg caggacatct tcctgaccga
    18301 gaccgcctgg ctggccgacg tggtgctgcc cgccagcgcc tggcccgaaa agaccggcag
    18361 cgtcagcaac accgaccgca tggtgcagct gggccggcaa gcgctggcgc cgccgggtga
    18421 agcacgcgcc gacctgtgga tcatccagca gctggcgcag cgcctggggt tgccctggaa
    18481 ctatgaaaac gatagctgct cgcgcttgcc agacaagcgc caggggccgt ttttccttga
    18541 agaaccgcac ggcgtcgcgg cggtctatga agaaatgcgc cgcgccatgc acggcgccat
    18601 cgccggcatc ggctgggacc ggctggtgcg cgaatccagc gtcacctacc cctgcctgag
    18661 cgaggacgac cccggccagc cgatcgtctt caccgagcgc gtgcccacgc ccaacggccg
    18721 cgtgcaactg gtgccggccg acatcatcgc gcccgacgag cgccccgacg ccgactaccc
    18781 catggtgctg atcaccggcc gccagctcga gcactggcac accggcgcca tgacgcgccg
    18841 cgccgtcgtg ctggatgcgc tggagcccgc gcccaccgtg tccatgcacg gcgacgatct
    18901 ggcgcggctg ggcctggtgg cgggcgagct ggtgcgcgtg agctcacgcc gcggccgggt
    18961 ctgcctgacg gcgcggcgcg acgacggcac gccggtgggc ggcgtgttca tccccttcgc
    19021 ctttcgcgaa gccgccgcca acctgctgac caacgccgcg ctcgacccct ttggcaagat
    19081 ccccgagttc aagtactgcg cggtgcgggt ggagccgctg gcgtgacggt gcacctcacg
    19141 cgcgccagcg cgccgctcac cgaacgcgtg ctggcggtcg acgagctggg tcaggcggtc
    19201 gaggtgtcga tcccggccga gcgggcgctg acgctgtacg tcgacaagcg cgagctggtc
    19261 acgctgatga ccctgggcgc ccaccccgaa tggctggtgc tgggctatct gcgcaatcag
    19321 cggctgctgg cggggccggc cgagctggct tcgattacgg tggactggga cgctggcgcg
    19381 gcggcggtgc gcacgcggca cggcatcgcc gacctggaga tgcgcacgcg ccggcgcacc
    19441 gtcaccagcg gctgcggtca gggcagcgtg ttcggtgacc tgatggccga tctggaggcg
    19501 ctggacctcg gcgccggccg gctggcgctg gacgatctgt acggcctgct gaacgccatc
    19561 cggctgcagg agagcaccta caaatcggcc ggttcggtgc acggctgcgc gctgttcgag
    19621 gggcagcgca tgctgctgtt cgtcgaggac gtgggccgcc acaacgccat cgacaccatc
    19681 gccggctgga tgtggctgca aggggacggc ggcggtctgc gcggcgacga caaagtgttc
    19741 tacaccaccg gccgcctgac cagcgagatg gtgatcaagg cggcccagat gggcgtgccg
    19801 atcgtggtct cgcgcagcgg catcacgcag atgggtctgc aactggcgcg ccggcttggc
    19861 ctgtgcgccg tcgggcgggc gctgaaccgg cgcttcctgt gctacagcgc gcccgagcgc
    19921 ctgggcctgc cggtgccgcc gcacggcgcc tgagcgacgt cagccctggc cctgcatcgc
    19981 gcggatcagc gcctgcgcct cggcgccttc ggggatcggg cgaccgctgt cgcgccactc
    20041 gacggcggac ttgaagccgt gctgctgcgc atggcggcgg aaccacacgc cctcggggtt
    20101 gtggcgcgtg atgccgtcga acaccgtggc cagcatctgc gtctgctcca gccccatggt
    20161 cagcatgacc tggttgacca ccagcttgtg catctgcagg tgaccgcgcg gcacgccggc
    20221 gatgcgtgcg gccaggcgca gggtggcggc ttccagctcg gcggccggca ccgcctggtt
    20281 ggccaggccc cagtcggccg cggtgcggcc gtcgatcacg tcgccggtga acatcagttg
    20341 cttggcgcgc gtcgggccca ggcggtaggt ccacatggcg gtggtggggc agccccacac
    20401 gcgcgtgggc atgtagccga tgcgtgcatc ttcggccatc accagcaggt cgcagcacag
    20461 ggcgatgtcg ctgccgccag ccaccgccgc gccatgcacc ttcgcgatgg tgggcttggg
    20521 gcagcgccac aggctcatga aatcttcggt gaagcgcttc atggcgcggt agtcgagcgc
    20581 cgggtcccag ggggccgatt cctgctggca ggggtgttcg atgtgttgct cggcggtggc
    20641 cgccaggtcg tagccaccgc agaagccctt gcccgcgcct tcgaccacga tcacatgcac
    20701 ctcgtcgtcg gccaccgccc agtcgaccgc ggcacggatc tcgcccggca tgtcgtcgtt
    20761 gatggcgttc aggcgctccg ggcggttgag cagcaggcgc gcgatgcgcg gctgggccgg
    20821 gtctttgtcg atgcgcaggg tgctgaagat gggcatgggg ctctcctgag ggcgggcgtc
    20881 catggtaggc ccgccgcgcg cgggcggctg tcgctttgcg tacagtgcgg ggcatgaacc
    20941 tgtcgtcctg gactctgggc tggcgcacgc tgtggcgcga tttgcgcgcc ggcgagctgc
    21001 gcctgctgat ggtggcggtc acgctggcgg tggcggcgct cagcagcgtg ggcttttttg
    21061 ccgaccggtt gcagggcggc ctgcagcgcg acgcgcgcca gttgctgggc ggcgatgtgg
    21121 tgctggtgag cgatcacgcg ccggcgcccg gctggacgca gcgcgccgcc gccgaggggc
    21181 tggacagcgt gctcacgctg gggtttccca ccatgggccg ggcgcccgag gcgcagggcg
    21241 gcgccacccg gctggtggcc ctgaaggcgg tcgagcgcgg ctacccgctg cgcggccggt
    21301 tgcgcctggc gcagcacgcg caggaccgcg ccggcacgcc gaccgacgcc gtgccggcgt
    21361 ccggtcaggt gtgggtcgat ccgtcgctgc tggaagcgct ggcgctgcgg cgcggcgaca
    21421 ccctgctgct gggcgacagc gcgctgcgca tcaccgaact gatcgcgctg gagcccgacc
    21481 gcggcaccgg cttcatgagc ttcgccccgc gcgtgctgat gaacgccgcc gacctgcccg
    21541 ccaccggcct ggtgcagccg gccagccgca tcacctggcg cctggcgctg gccgggcccg
    21601 agccggccgt gcagcgctac cgtgcctggg ccgaggccga gctggcgcgc cccggcatgg
    21661 ccggcctgcg gctggagacg ctggacaccg gtcggcccga gatgcgcacc acgctggatc
    21721 gggccgccaa gttcctcaac ctggtggcgc tgctggcggc gctgctgtcg gcggtggcgg
    21781 tggcgctggc ggcgcgcgcg ttcgcggcgc gccacctgga cgattgcgcc atgctgcgcg
    21841 tgctgggcct gccacagcgg cgcatcgccg gcgcctacgt gaccgaattc ggcctggtgg
    21901 gtctgagcgc cagtctggcc ggggtgctga tcggctggct cgtgcaccac atcttcgtgg
    21961 ccctgctggc cggcctggtg gacagcgcgc tgccggcgcc cggcccgtgg ccggcgctgc
    22021 tgggcctggg catcggcatg acgctgctgg tggcctttgg cttgccgccg gtgctgcaac
    22081 tggcgcaggt accgccgctg cgcgtgctgc ggcgcgacgt cggtgccctg cggccggcgt
    22141 cgctggcggt gctgggcctg ggcgtggccg gctttgccgc gctgctgctg gcggtcagcg
    22201 ccgatctgac gctgggtctg atcgcggtcg gcggcttcgc cgtggcggtg gccctgttcg
    22261 ccctgctgtc ctgggtcgcg gtgcggctgc tgcggcggtg ggtccacgag cagcgtgcgc
    22321 cgcgctggct ggtgctggcc acgcgccagc tgtcggcgcg gccggcctac gcggtggtgc
    22381 aggtcagcag cctggcggtg ggcctgctgg cgctggtgct gctggtgctg ctgcgcaccg
    22441 atctgatcgc cagctggcgt gccgccaccc cggccgatgc gcccaaccgc ttcgtcatca
    22501 acatcatgcc ggagcagggc gaggcgtttc gcgccgtgtt gcggcaggcc ggcgtcacgc
    22561 gcatggactg gtacccgatg ttccgcggcc ggctggtggc catcaatggc cagccggtga
    22621 atccggccga ctaccccgag gagcgcgcgc gccatctggt cgatcgcgag ttcaacctct
    22681 cgcacgcggc cgaacgaccg gcgcacaacg acatcgccgc cggccactgg accaccgacg
    22741 aggctggcgc catcagcgtc gagcagggcc tggccgagac gctgcgcctg cggctgggcg
    22801 accggctgcg cttcgacgtc gcgggcatgc cgctcgaggc gcgcatcacc tcgctgcgca
    22861 aggtcgactg ggcctccatg cacgtcaatt tctttgccat gttcccgcgg gccgacatgc
    22921 ccgacgtgcc ggtcacctgg atcgctgcct accgcgcgcc cggacagccg ggcttcgacc
    22981 acgcgctggt gcgccagttt cccaacatca cacacgtcga catggagcag tccatcgccc
    23041 aggtgcagcg cgtgctggac caggtggtgc gggcggtcga gttcctgttc ggcttcacgc
    23101 tggcggctgg gctgatcgtg ctgtttgccg ccgtcagcgc cacgcgtgag gagcgcgcgc
    23161 gcgaattcgc cgtcatgcgc gccgtcggcg ccggcagtgc gctgctggcg cgtgtgcagc
    23221 aggccgaact ggttggcgtc ggcttgctgg cgggggtgct ggccagcgcg gtggcgatgg
    23281 gcatcggctg ggccatggcg cgccatgcct tcgacttcca ctggaccgcc tcgccctggg
    23341 tgccgctgct gggcggcgtg gctggcgccg ggctggcact ggcggccggc tggtggggct
    23401 tgcgcgaagt gctgcagcgg cccgtggtgc agacgctgcg gcgggtcgcg gagtgagcgg
    23461 ccggatgcgc cggctgcgct gacgcgggcc gccgccgctg cacaggcgcc ggggcatggc
    23521 cgcgtgtgtc gcgcaaaaaa acccgcgcaa cgcgcgggtc ttcggggcgc cgttggctca
    23581 accgatgtcg ccggtggccg ggcggaaacc ctgcgccatg gcgtcgcgca gttcggccac
    23641 caccgtttcg cggtccaccg gctgcggcgt gggcgccgtg gtctgactcc cgttgccctc
    23701 accgctggcc ggtggcgatt ggatcgccat cgccatcacc tcagcgcgcg tcaggtcgga
    23761 ggggcctcgg atcgggtcgt tgaagaacgg accttcgccg ctggcgggca tggcggccga
    23821 tgcggcgccg gcgatcagcg tccacgccag ggtcgaagcc aagggaaaga aacgtgcgga
    23881 catgatgaaa ctccttgaaa ggttcatgaa gcgatggcaa cagaagaggc aatgcgtaag
    23941 taccaccttg ttccgtgaaa gaggccccgc tccaatggcc tcttccatgc gacgtactgt
    24001 gcgacttgca aggcgctgaa taaagtcgtc gcatggaatg tcgaccattc caggccgtga
    24061 acaatggata cgctggtgct cctgcgcacg tttcgagagg tggccctgcg ccacaacttc
    24121 tcgactgccg cgcgcaccct tgatctggcg ccggccacgg tcagcaagta cgtggccgaa
    24181 ctggaggcgc gctttcacct gcgcctgttc catcgcagca cgcgccgggt gacgctgacc
    24241 gacgccggcc agttgctgct ggatcattgc gcgcaggtga tcgacctgat cgatcggacg
    24301 cacgacgcca tgaacgagcg tgcgcagcgg cccagcggac ggctcaatct gacggcgccg
    24361 catgggctga cgcgcacgat cctggtgcgc cgtctggcgg atttcatgct gcagcattcg
    24421 gatgtgtcga tcaacctgaa cctgaccaat cgcatggtcg atctggttga agatggtgtc
    24481 gacgtcgcct tccgcgtcgg ggcggtggcc gacaacagcc tgatcgtgcg ccgcctgctg
    24541 cccctggaga tggtggtggc ggccacgccc gcctactggc gcgcgcacgg gcgtcccgaa
    24601 cacccgcgcg aactggccac ccatcgcacg ctggccatgg caccgacggg cgagacgccg
    24661 cactggcagt tcagcatcgg cggcaagctg ttcgacctgc cgctgcaccc ggtgttctgt
    24721 gccagcgact cctcaccgct ggtgccgctg gcgctgcagg gcgtgggtgt gctgcgtggc
    24781 tctcgcatgc tgctgggcga atggatcgac gatggcaggc tggagccgct gtttgcagcg
    24841 tattcaccgc gcaacctgtg gctgtacgcc gcctatgccc agcgccgcca ccccagtgcg
    24901 gcgttgcggg cgctgctgga ttttctcgag cagcagatgc gtcccaccgt cccgtcggtc
    24961 ccgcatgccg tttcgccgag gctgcactga gcctgaactc ggccatggag cgcgcaatgg
    25021 tcggtctcgg gccaagcgat ctgcatcgaa tggccggtgt ggcttgtggg gataagcgcc
    25081 ggcagctact gaaatcatag taaactgggg catggcccag ctcttcaccg tgcaccccga
    25141 caacccgcag ccgcgcctgc tcaagcaggc ggcggcgttg ctgcacggcg gcggcgtgct
    25201 ggccgtgccc accgattcca gctacgccct ggtgtgccac ctcgacgaca aggcggcggt
    25261 cgatcggctg cgccgcatcc gccaggtcga cgagcgccac cacctgaccc tgctgtgccg
    25321 cgacctgagc gagctggcca gctacgcccg cgtcgacaac acgcagtttc gccgcctgaa
    25381 gagtggcacg ccggggccgt tcaccttcat cctggaagcc agcaaggaag tgccgcgtcg
    25441 cgtcagccat ccgcagcgca agaccatcgg cctgcgcgtg cccgatcacc ccgcactgcg
    25501 tgagctgctg gcgctgcatg gtgcgccgct gctggccacc acgctgatcc tgccggggga
    25561 gggcgagccg ctgaacgacg ccggggcgat cctggagcag ctgggccatc agatcgccgg
    25621 cgtgatcgac gccggcgcct gcccgctggc gcccaccacc gtggtcgacc tgagtggcga
    25681 cgcgcccgag gtgctgcgcc gcgggagggg cgatccggcg cggctggggc tgtgattcgg
    25741 cgcctggtca cggtctgaga caatccacgc cgtggacttc gccaacatca ttcagaccct
    25801 cgccctctat gccctgccgg tgctgtttgc catcaccgtg cacgaggcgg cgcacggtta
    25861 cgcggcgcgc catttcggcg acggcacggc ggccttcctg ggccgcgtca cgctcaatcc
    25921 ggcgcggcac atcgacccca tcggcaccat cgccatgccg ctgtttctgt acatcgccac
    25981 cggtggtgcc ttcgtgttcg gctacgccaa gccggtgccg gtcaacttcg gccagctgcg
    26041 ccaccccaag cgcgacatgg tgtgggtggc gctggcgggg ccggcgtcca acctggcgat
    26101 ggcgctagcc tggggggcgc tgctgatcgt gctcgacggc gtgggggtga ccgagccgtt
    26161 ttttctcaag atgtgccagg ccggcgtgct ggtcaacgtg gtgatcggcg tgttcaacct
    26221 gtttccgctg ccgccgctcg atggcgggcg catcctggtg gggctgctgc cgcgcgggcc
    26281 ggccctggcc ctcagccgca tcgagccctg gggctttttc atcgtcatgg cgctggtggt
    26341 ggcgggcgtc atcagcaacc tgtggatgcg gccactgatg gcactgagct ttcatctgct
    26401 cgatttcatt cttgcgccgc tgcaggcgct gttcggctga tcgcatggct tcttctgttt
    26461 cgcgccgggt cctcaccggc atcaccacca ccggcacccc gcacctgggc aactacgtcg
    26521 gctcgatccg tgccgccgtg cgcgccagcc gcgagccggg ggtgcagagc ttttacttcc
    26581 tggccgacta ccacgcgctg atcaagtgcg acgaaccggc gcgcatccag cgcagcacgc
    26641 tggaaatcgc cgccagctgg ctggccgccg ggctggatcc cgagcaggtc accttctacc
    26701 gccagtccga catcccggag atccccgagc tcacctggct gctgacctgc gtcaccggca
    26761 agggtctgct caaccgcgcc cacgcctaca aggccatgct cgacaagaac gccgccaccg
    26821 gggccgatcc ggacagcgac gtcaccgcag gcctgttcat gtacccggtg ctgatggcgg
    26881 ccgacatcct gatgttcaag gcgcaccagg tgccggtcgg gcgcgaccag atccagcaca
    26941 tcgagatggc acgcgacatc gccgccagct tcaaccacct gtatggcgaa ctcctgaccc
    27001 tgcccgaagc gctgatcgac gacaacgtgg ccctgctgcc cggcctggac gggcgcaaga
    27061 tgagcaagag ctacgacaac accatcgccc tgttcggccc gcgcgagcag ctgcgccggc
    27121 agatcatgag catcgtcacc gattcgcgcg cgccgggcga acccaaggac accgagggct
    27181 cggcgctcta ccagatctac cgcgcctttg ccgatgacgc cgaggcggcg cagtttgcgc
    27241 gcgcctttgc cgacggcatc gcctggggcg aggccaaaca gcgcctgttc gagcgcatcg
    27301 acgccgagat cgggccgatg cgcgagcgct accagcacct gatgtcccat ccggccgagg
    27361 tcgaggccct gctgcggcgc ggcgccgaca aggcgcgcgc gctggccacg ccgttgatgc
    27421 gcgagctgcg ccaggccgtg gggctgcgtg cgctcgacac ccaggtggcg gcgccgcgcg
    27481 ggcgcgactc ggccaggacg ggcctgccca gcttcaagca gtaccgcgag caggacgggc
    27541 gcttttactt caagctcacc ggcgccggtg gccaggtgct gctgcaaagc cggggcttcg
    27601 aatcgccgcg cgaggccggc caggccgtca agcgcctgca gagcgagggc gagcacgccc
    27661 tgggcgcgct ggccgaatgg ctggaaccgg tggccgacgc gctggcgccg accctgaccg
    27721 aggcgctgca ggcgttgcag accgcacaac aaagctgatc cgctgcgccg ggcgcacggg
    27781 cctggcgccc cgcctgctcg catcggcggg cggactggtg cacaatcgca acctatacac
    27841 aaaaaggttt aggagcagcg atcgtggcga tctatgtcat cgacgaccat cccctcatgc
    27901 gcgaggcggt ggtgatgctg ctgcggcgca ttcgaccggg ttcggaggtg gtcgagctgt
    27961 cgcagctgtc ggatctggag cgtgcggtgg aaacccgcgg cgcgcccgag ctgttctgcc
    28021 tggatctgaa actgcccgac accgtgggca tttcgggcgt caagctgctc aagcgcctgt
    28081 accccgacgt gccgctggcg gtgatctccg ccacgcccgc cgccgatgtg gaggagtggt
    28141 gcaccgaggc cggtgccgac gtctacatcg agaagtcggc cggcgccagc gaaatcggcg
    28201 ccgccctgcg cgccatgctg cagggtgaca gcgagttcgc cgacctcgaa tacggcgaca
    28261 gccagaccga aaccaaactg tccaagcgcc agaaacagct catcgtgatg ctggacgaag
    28321 ggctgtccaa ccgcgacatc gccgagcgcc tgggcatcag cgagcacacc gtcaaggtgc
    28381 atctgtggcg gctgtttcgc cgcctgggcg tcaagagccg gacccagacc ctgcacttcg
    28441 ctcgcacgca cggcctgctg agttcctgag ccccgccggc gccggcaccc agacgcggaa
    28501 cacggtcccg cgcccgggcc gcgagcgcac cgtgacgcgg tagcccagcc gctcggccag
    28561 cgcctgcacg atcgacaggc ccaggccgaa gccgtcttcg gtgccgctgg tgggcacctt
    28621 gtagaactcg ccgaagatct gttccagctc gctctgggcg atgccgatgc cggtgtccca
    28681 cacctccagg ctgaccccgt cggggcgcgc gcgcgccgcc agcagcacgc cgccgcgcgc
    28741 ggtgtagcgg atggcgttgg ccagcaggtt gccgatcacg cggcgcagca tcaccgggtc
    28801 ggtgcgcagc accacgtcgg ccgggcgcac gcgcagccgc agccccttga gccgggcttc
    28861 ggggccgaac tgcacctcca ggtccgacag caggggacgg atggcgacct cgcgctcgcg
    28921 cagctcgtaa tggccggcgt ccagccgcac ccagtcgaac agcgagtcga acaggctatg
    28981 gatggccagg gtcgattgca ggatcttggg cgccagctca tcgaccagct cgggttcgtt
    29041 gctcagccag tcggcgtaca ggccgacggc gttgaccggc tgcttcaggt cgtgcgccgc
    29101 ccccgccaga aagcgggtgc gaaagcgtgc cgcctcctgc gccacgcggg tctgctcgcg
    29161 cagcgactcg atcaggcgcg cgttgcgcag gctcagctcg atgccttcgg ccacccgctg
    29221 gtgctggctg ccgatgatgc gccacaggat ggcggcatac accagcagca acagcgcctg
    29281 ccaccacagg ccgcgcaggt cggcgcgcag cggcgagccg ctcaggcgca gccacaggct
    29341 ggccagcagg gccgccacca gcacgctggc cagaccctgt gccagccgcc ggtgcgccga
    29401 cgaccacgac accagcagca cccccgtgcc cagcgtcagc gcccagcaca tggcctccac
    29461 ctccggcgcc acgcgctggg caaagcacag cggcgccatg ccccagacca gcgcctgcac
    29521 cagccagctg gcgcgctggc ggcgcaggcg cagccgctgt tcggccagcg gcgcccaggc
    29581 cagccgctgc cggtagctgc gccagccgcg ccagcccggc gccatcacca gcagggcggc
    29641 gcccagccac aggccggtgc gcaggccggg cgcgtgcagc agcagcagcc cgcccagcag
    29701 cgcccacagc gccagcaccg ccagctgggt gcccggccag ccgtccatca ggatggcgag
    29761 ctggcgtgcc tcggtccagt cggcgcggct ggccgggtcc ggggcggggg ccgggatggg
    29821 gatcacgagg tgctattcat tcaatagctg cttgcgcttt ccagtcaagc gctggagcct
    29881 tgtttgatgc ctaaagacgg ccacactggg cgcgctggcg cagcgcgtgg tccatcacca
    29941 ccagcgccag cagcgcttcc agaatgggcg tggcgcggat gccgacgcag gggtcgtggc
    30001 ggcccttggt gatgacttcc accggctcgt ccgccaggtt gacagaggcg cgcggcacca
    30061 ggatcgagct ggtcggcttg atcgccaccg cgacctcgat gtcctgaccg ttgctgatgc
    30121 cgcccagcac gccgccgtcg tggttgctgg caaagccgcc gccgggcagc ggcgagtcgc
    30181 cgtgctggct gccgcggtgc gccacgctgg caaagccgtc gccgatctcc acccccttga
    30241 cggcgttcag ccccatcatg gccttggcga tgtcggcgtc cagccggtcg tagatcggct
    30301 gacccagccc caccggcacg ccgcgcgcca ccacgcgcag gcgcgcgccg cacgagtcgc
    30361 ccgccttgcg cagcgcgttc atgtggtcct ccagcgccga cacgtcggcc accggggcga
    30421 agaacgggtt gcgcggcaca tgcgcccagt cctcgaagcc gatggccagc tcgcccaggt
    30481 gcgtcatgca gccggcaaag ctggtgccgc actgctcgcg cagccacttg cgtgccaccg
    30541 cgccggcggc cacggtgggc gcggtcaggc gtgccgagga tcggccgccg ccgcgcgggt
    30601 cgcgcaggcc gtacttcttc cagtaggtgt agtcggcgtg accggggcga aagctttgcg
    30661 cgatgtcgcc gtagtccttg ctgcgctggt cctggttgcg gatcagcagg cagatcggcg
    30721 tgccggtggt gtggccctgg tagacgccgg acaggatctc cacctggtcg ggctcctgcc
    30781 gctgggtgac gaacttgctg ctgccggggc ggcgccggtc cagctcgggc tggatgtcgg
    30841 cctccgacag cgccaggccg ggcgggcagc cgtcgatcac gcagccgatg gccgggccgt
    30901 gggattcacc gaaattcgtg acggcaaaca gagtgccgag ggtgttgccg ctcatggggc
    30961 gccattatcg ccgctggcgt ggcgtcggcc gggccggccg ccagaccccc ggattcatgg
    31021 tgacaaaggc cagaaacctt ggtgcagcaa gcgccggcag ctcctgaaaa cgcagcaacc
    31081 gaggcgcctc aggcgcgctc ggtggcggcc ggcgcggcgt cttcctcggg ccagtcgcgg
    31141 atgtaggcct tgagcatctg gttctcgaag tcctgcgcgt ccaccaccgc cttggccacg
    31201 tcgtagaagc tgatcacgcc catcagctgg cggttgtcga tcaccggcag gtagcgcgtg
    31261 tggtgctcca gcatcatgcg gcgcacctcg tccagctcgg tctccagggt gcagatcagc
    31321 ggtcccgggt ccatgctgcg gtacaccgac aggccggcca gcgagccacc gttcttcacc
    31381 gtggcctgga tcacttcgcg gaaggtcagc atgcccacca cgcgctcgtg cgacatcacc
    31441 accagcgagc cgatgtcacg gtcggccatg gcctgcgcgg cgtcggccag gctgtcttcg
    31501 ggcgtgatgg tgtagagggc gttgcccttc aggcgcagga tgtcgcggac tctcatggcg
    31561 tggctttcgg tggcgccggc gggccggcgt cgcaggaata tagcccacaa tacggcgcgg
    31621 cccggcccgc gcgccgcacc ccttgccccc tgaccaggag accccatgcc cggctacgcc
    31681 gaccccggtt tcgacacgct cgcggtgcac gcgggcgcgc ggcccgaccc cgccaccggc
    31741 gcgcgcgccg tgccgatcca cctgagcacc tcgttcgtgt tcgactccag cgaccacgcc
    31801 gcccagctct tcaacatgga gcgcgccggc cacgtctaca gccgcatcag caaccccacc
    31861 aacgcggtgc tggagcagcg ggtggcggcg ctggagggcg gcatcggcgc catcgccacc
    31921 gccagcgggc aggccgcgct gcacctggcc atcgtcaccc tgatgggcac cggcggccac
    31981 atcgtcgcca gcagcgcgct gtacggcggc agccagaacc tgctgcacta cacgctgcgg
    32041 cgcttcggca tcgaaaccag cttcgtgcgg ccgggcgatc tggacggctg gcgcgccgcc
    32101 atccgcccca acacgcgcct gctgctgggc gagaccctgg gcaacccggg gctggaggtg
    32161 ctggacatcc ccgccgtggc gcagatcgcg cacgcggccg gcgtgccgct gctggtcgat
    32221 tcgaccttca ccacgcccta cctgctcaag ccgctggagc acggcgccga cctggtctac
    32281 cactcggcca ccaagttcct gtccggccac ggcacggtga tcggcggcgt gctggtcgac
    32341 ggcggcagct tcgactggga ccgctccggc cgttttcctg agctgaccac gccctacgag
    32401 gggtttcacg gcatggtgtt tgcggaagaa agcaccgtcg gcgcctttct gctgcgcgcg
    32461 cggcgcgaag gcctgcgcga cttcggcgcc gcgctgtcgc cgcacagcgc ctggctgatt
    32521 ctgcagggca tcgaaaccct gccgctgcgc atggagcggc acatcgccaa caccgagcag
    32581 gtggtgcgct ttctggccga gcacccgttc gtggcgcgcg tcggtcatcc gctgctcgca
    32641 tcgcacccca gccacgcgct ggccgaacgc ctgctgcgcc acggcgcgcg cggcgccggc
    32701 agcgtgttca gcttcgacct gaagggctcg cgcgcccagg gcagggtgtt catcgacacc
    32761 ctcaagctgt tcagccatct ggccaacgtg ggcgactgcc gcagcctggt gatccacccg
    32821 gccagcacca cccacttccg catgaacgac gaggcgctgg ccgcggccgg catcgggccg
    32881 ggcaccatcc gcctgtcgat cggcctggaa gatccgcagg acctgatcga cgacctcaag
    32941 cgggcgctga aggcggccga gaaagcgggg gcctgagacc atgttcatcg acctccacgg
    33001 cgctcccctg tacgcctaca ccggcgggcg ccccttcgat ccggcgcagc cgaccgtggt
    33061 gttcatccac ggcgtgctga acgaccacag cgtgtggatc ctgcagtcgc gcgccctggc
    33121 gcaccacggc tggaacgtac tggccatcga cctgcccggc cacggccgca gcggcggcca
    33181 gccgccggcg acggtggaac aggcggccga cgacgtgctg gccctgctgc gcgcggccgg
    33241 catcgcccgg gcggtgctgg tgggtcacag cttcggctcg ctgatcgcgc tggaagcggc
    33301 ggcgcgcgcg ccgtcccagg tcagccacct ggtgctggtg ggcacggcct ttccgatggc
    33361 ggtttcgccg gcgctgctgg aagcttcggt cagcgcgccc gaaaaagcca tccacatggt
    33421 caacgtgttc tcgcacagca cgctgtgccc gccgccgtcg gccctgggcc cgggcacctg
    33481 gctgtacggc agctcgcgcg ccctgatgcg gcgggtgctg gccagcaacc cgacggtcaa
    33541 tctgtttcac accggctttc aggcctgcga ccgctaccgc ggcggcgaac aggccatggc
    33601 ggcggtgcag tgcccgacgc tgctgctggt cggtcgccac gaccagatga cgccgccgcg
    33661 cgccgcgcgt gccctggccg cccatgcaag gcaggccacg gtggtggaag tggaggccgg
    33721 tcacgccctc atgaccgaag cgccggacgc ggtgctggac gccctgcgcc agttcctgag
    33781 caggacctga gggccgctgc cgcgcggccc tgaattacta ttgatttgat agctgctggc
    33841 gctttccaga cggtcgccag agcgcgattt gacttgatgt ctggtgtggc ctcctcaggc
    33901 gcagggcgcg cgcgccgggg cgggccgcca tgccgcctgc aggtgctggc gcagccagcg
    33961 cagcaggccg gcgcagtgcc cttcgtcggc ctgccagttg aagctggctg gcggcacctg
    34021 cgccgccggg gtggccgcca ggtgcatcgg ctcgatcacc accgagtcgc cggcatccaa
    34081 cgccagcgcc agaccggcgc tcacgtacag gtcctgcgcc gggtggtcga cgtggcgccc
    34141 gcgccgcgtc acccacagcg cgccctgcgc cacgcgcagg gtgccggcgg cgggcaggtg
    34201 cagggtgcgg gcctggccgg gttgcagcgt gatgctcacg ccgcgcgaca gtggcagacg
    34261 gggcgaagaa ggcaggcggg cgggtgacat ggcggctcct gaagcgacgg tcgaggaatc
    34321 gatcgggtga cccgatgatg gccctggcgc gattgacagt ccaatgaatt gcggtcaggc
    34381 tattgatgca ttcaaggcat caatccagca tgttgcacgc gcccacccat ctgcgctcgc
    34441 gtcccatcca ggtcggccac ctgcgcgcct tcgaggcggt ggcgcgccac ctcaacttcc
    34501 gcctggcggc ccaggagctg gcgctcaccc agccggccgt cagccgccag atccaggcgc
    34561 tggaggacga cgtgggcgtg ccgctgtttc ggcgccatac gcgcgccgtg gagctgaccg
    34621 aagccggcaa ccagctgctg accgcggtgg ggcaggcgct gccgcgcctg gacgccaccg
    34681 tgcgccacat ccgccagagc gccgggcggc gccatgtgtc ggtcaccacc tttgcctcgt
    34741 ttgcctcgat gtggctgatc cccaggctgg aagcgttcca gcgcgaccac cccgacatcg
    34801 acatccgcgt cgaggccagc gacacggcgg tggacctgga ggtgaccgac atcgacgtgg
    34861 ccgtgcgcta cgccccggcc gagcgcatgc cggcgccggc gcagcgcctg ttcggcgaat
    34921 cggtcacgcc catggccagc ccctggctgc tcaggagcgg cccgccgctc aagcgtcctg
    34981 aagacctgct gcgccacacc ctgatcgagg cgggcgacgc ctacaccccg cacctgcaat
    35041 ggctgacctg gcggcgctgg ctcgacgcca acggcctgaa ggacgcccag cccaagcgct
    35101 ggctcagctt caactacgcc taccaaatgg tgcaggcggc gctgtcgggc cagggcgtgg
    35161 tgctggcgcg cccgccgctg gtggccgaga acctggcgcg cggcgatttg gtggaggtgt
    35221 tcgccgccgg cgcctacccg caggcgcggc tgatatcgcc catggcctac tgggtgctgg
    35281 tgtcgccgca cagccgccag cgtgccgagg tgcgcgcctt tgccgactgg ctgctgatgc
    35341 aggcgcaaag cacgcgctag ttgcggtggt tcgcgctgtc gggcgcttat agaagtcaga
    35401 aaaaggcttg cggcaaggcg cggctcgcag cccatgcttg gcggccttgg caacaggcgc
    35461 aacgccgccg caagccgttt tctggcttcc cgcaggtcgc gccgccaagg ggccatctgc
    35521 ttggtggcgt ggccttgaca tggcgccacc atgcctgcgg ccggcggcgg cggctacagc
    35581 ccgtccagtt cgcggtggcg
//
LOCUS       DAOQTZ010000012        34887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1418_length_34887_cov_13.857172, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000012 DAOQTZ010000000
VERSION     DAOQTZ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 34887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34887
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1418_length_34887_cov_13.857172"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..802
                     /locus_tag="PKN51_02800"
     CDS             140..802
                     /locus_tag="PKN51_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT84057.1"
                     /translation="MTATTLSALRYLTVPVTPFAQNCSIVWCERSRQAAIIDPGGDLP
                     RIEAALRELAVTPTQIWLTHAHIDHAGGSAELSARLGLPIVGPHPGDQFWIDGLAEQG
                     RMFGFARAEPFTPTRWLHDGDTVRVGDCELQVRHCPGHTPGHVVFHSPEARRAFVGDV
                     LFAGSIGRTDFPGGNHAQLIASIRERLWPMGDDTVFIPGHGPESTFGEERRHNPFVRD
                     GL"
     gene            792..2159
                     /locus_tag="PKN51_02805"
     CDS             792..2159
                     /locus_tag="PKN51_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HNT84058.1"
                     /translation="MASEAPAAGRPRRDWRASLRIYLSPPSLRMLALGFAAGLPLLLV
                     LGTLSFRLREAGIDRSTIGYLSWVGLAYGFKWAWAPLVDRVPLPGLTRLLGRRRAWLL
                     LAQLGVMAGLVAMGWADPRVSLAAVVWSALAVAFASATQDIALDAYRIESAPTDAQPA
                     LAASYQTGYRLAMIWAGAGVLWLAARAQGDASGYQHEAWRQAYLVMAASMLPGLLTLL
                     RSPEPAPQPLAPARSVGEWLRTALIEPFADFLRRYRWQAALILALIAVYRISDVVMGI
                     MANPFYVDMGYTKDEVAAVTKVYGVAMTLLGAFVGGSLALRFGVMRILMLGALLSALT
                     NLLFAWLAGHGHSLPALIAVVSADNLASGMASAAFIGYLSSLTNLSYSATQYALFSSL
                     MLMLPKFIAGFSGDYVNAFGYAGFFTSTALLGLPVLLLVALAARVPTRHAGQNEPVAQ
                     AGGTP"
     gene            2215..2973
                     /locus_tag="PKN51_02810"
     CDS             2215..2973
                     /locus_tag="PKN51_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT84059.1"
                     /translation="MDSFSNLAVLALAAFGAGVLNAIAGGGTFLTFPALVWTGVPPIA
                     ANATSALAVCPGYLGSALGFRAELAALPRALRLREVALSALGGVAGALLLLVTPAKVF
                     AAVVPWLLLFATVLFALGPRLAAWAGGDHGGGLARWRLPALLAVALYGGYFNGGLGIL
                     LMALYLLVGETRMNTVNALKNLNSFVLSVVSVVAFALGGAIVWRQALLMAVFATLGGF
                     AGARLARRLPVAWVRAIVIVTGLAMSALVFARSS"
     gene            complement(3120..4226)
                     /locus_tag="PKN51_02815"
     CDS             complement(3120..4226)
                     /locus_tag="PKN51_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT84060.1"
                     /translation="MHGRLRKLALTTLLCLGGAAQADILIGQTTAVTGPVAASVGETL
                     LGVRAYLDHVNAQGGVHGEKIKLITLDDNFKPPLAAENARKLIEQEGVVALFMSRATP
                     HTQAILPLLAKHKLALVAPSTGAMVFHRPVNPYVFNVRSSYQAEAERAIEHVFQVTLE
                     RVAVVHVDDSFGADCLEGAQKGFERAGKKPVAVIKADREKPDYPAIVKQLTDANAQAV
                     LWIGSSVAVSDGVKALRQAGSAAQVLTVSNNASGGFIKQLGEHARGVVVSQVFPSERA
                     LGIPINREAVALLAGKGELTPQVMEGFASAKVLVEALRRAGPKPTREKIIDALNGLQR
                     FDLGGLELGFSATDHTGLSYVELSIIGRDGRFKR"
     gene            4392..5561
                     /gene="mnmA"
                     /locus_tag="PKN51_02820"
     CDS             4392..5561
                     /gene="mnmA"
                     /locus_tag="PKN51_02820"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNT84061.1"
                     /translation="MQTKQRIVVGLSGGVDSAVAAWLLRQQGHDVVGIFMKNWEDDDD
                     SEYCSSNVDFVDAAAVADVIGIELEHVNFAAEYKDRVFAEFLREYQAGRTPNPDVLCN
                     AEIKFKAFLDHAMRLGAEKIATGHYARVRFNAATGLHELLKGLDPSKDQSYFLHRLNQ
                     AQLARTLFPVGELHKTEVRRIAAEIGLPNAKKKDSTGICFIGERPFREFLGRYLAQQP
                     GPIKDERGRTIGRHQGLSFYTLGQRQGLGIGGVKDRGAARGGGDHAPWFVARKDMARH
                     TLYAVQGHDHPWLLSRALVFDNASWIAGQPPAAGACSAKTRYRQQDAACTLSATAVAG
                     SWRLRFAEPQWAVTPGQSAVLYDGPVCLGGGVIAAAEEYEPDAATGLAQQAPEAM"
     gene            complement(5571..5846)
                     /locus_tag="PKN51_02825"
     CDS             complement(5571..5846)
                     /locus_tag="PKN51_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNT84062.1"
                     /translation="MPPGRPILDLAWLAMLVATAITWVLGRSGWVASAGLAGMALVFA
                     LAWLKGLAVILQFMELQHAPARWRRALIGALTLIVLLILLAYGLSLR"
     gene            complement(5860..6435)
                     /locus_tag="PKN51_02830"
     CDS             complement(5860..6435)
                     /locus_tag="PKN51_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNT84063.1"
                     /translation="MTPRHAPAPRLPGDQFIWYVIALELATFCVLFGAFAFTRLWHLE
                     LFRTSQQALDVSAGALNTALLLSASWAVARAVHATRADARRAALGWLGLGWLLGAGFV
                     VFKLREYADKAAQGIGMGDGNLFFDFYYILTGFHLLHVLAGLLALALTAVGLARQRGR
                     LQPHTLETAGAFWHLVDLLWLVLFPLVYVMR"
     gene            complement(6508..7527)
                     /locus_tag="PKN51_02835"
     CDS             complement(6508..7527)
                     /locus_tag="PKN51_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HNT84064.1"
                     /translation="MPQRPDALKYLAPNWFAMVMSVAGLAIAWARATPLLGELATAGA
                     LVLAAAAALLFVALLVLSWIRWQHHPEALAADLKHPVRHAFIATLPVSLILLASAAAM
                     LFGPESWVAVLWWLGSLTQFGVTLWVLARWLSADKTQGLSWAALTPVLFIPVVGNVLA
                     PLGGVMLGAPAWTAAQFGLGLLLWPVLLALIGARIATQGLWPERLLAVTFISVAPPSV
                     VGSGVLLLGGPLTLAWMCWGVALFFLLWSGQVLRRMLAQPFSIAFWSLGFPLAAFAAL
                     TLRLAQQDGAAQLPALVALALASLVVLVLGIATWKGWRQGSLLQPEPVAAMAATASAP
                     PPQAQ"
     gene            complement(7751..8698)
                     /locus_tag="PKN51_02840"
     CDS             complement(7751..8698)
                     /locus_tag="PKN51_02840"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HNT84065.1"
                     /translation="MSCLTSRWPRISMLMLCSLGTGGALAQTQTYATPGNYTYIVPAG
                     VTALQVTVAGGGGGGGGYDLLQTGNGGGGGSGALLQGRLPVTPGSTVQITVGGGGGGG
                     TSDSDGNPAAGGNAGAGLGSGGAGGAPGSTGGSGGGGGGGGSSSLVVGALALQAAGGG
                     GGGGGSGSLGGTAASGASAGGSAPSDANCATGTVGGTGGSPGGGDGGGGGGGGGGVTS
                     GSAGTAGQDGIAHGGPGGAGGSCHLGAVQGLTTSSAGGAGGAGGLASGTAAVTGTHGQ
                     VVITPLLATAVPTLGQWGLALLATVAALLAGRRLRRVAP"
     gene            complement(8747..9244)
                     /gene="ssb"
                     /locus_tag="PKN51_02845"
     CDS             complement(8747..9244)
                     /gene="ssb"
                     /locus_tag="PKN51_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNT84066.1"
                     /translation="MASVNKVILVGNLGRDPEMRSFPNGDQVANVTLATTDKWKDKQS
                     GEPREHTEWHRLVFNGRLAEIAGQYLRKGSQIYVEGSIRTRKWQDQATGQDRYSTEIR
                     VDQMQMLGSRQGMGGPSDDGGGDGYDAPRRSAPAPRAAPAPRAAAPAARPSSGFDDMD
                     DDIPF"
     gene            complement(9369..9794)
                     /locus_tag="PKN51_02850"
     CDS             complement(9369..9794)
                     /locus_tag="PKN51_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNT84067.1"
                     /translation="MTPAAPPPEPPPVVIDTNRVLDLWLFGDPEVAPLRQAIEAGRLH
                     WMAQPAMRVELARVLTYPAVARQLLRHRRSADAVLAAFDRWVQRVPAAPPAPVRCRDP
                     DDQIFIDLAVTWRARLLSRDRQIITLARRLTPLGVTVEA"
     gene            complement(9791..10774)
                     /locus_tag="PKN51_02855"
     CDS             complement(9791..10774)
                     /locus_tag="PKN51_02855"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT84068.1"
                     /translation="MPPIHNFSRSRRASLAALAASVAAPAVWAQQDRPIRIIVGFAPG
                     SANDLIARDLARHMAESLKQPVIVENKPGAGGSLGTEAVAKAAPDGLTLGLGTSSQMV
                     MNVALYQNLPFDVERDLRQIGLVGRTPLVLVGKAAGARTLKELIANARANPGQLSYGS
                     GGIGSISHIVGEAFARAADIRLNHVPYKGNGPAMADLAGGHVDMVFDGIITAQPMAQQ
                     GRVRLLAFGGKQRNAAIPDVPTFAEQGLRDYDAYTWNCLFAPAKVADADITRLNQALN
                     AALALPAMKERLARGGADNLGPSTPAQADAFGRKERERWVPFVRSLKLTIN"
     gene            complement(10795..12600)
                     /locus_tag="PKN51_02860"
     CDS             complement(10795..12600)
                     /locus_tag="PKN51_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HNT84069.1"
                     /translation="MNTGKIIRIGGASGFWGDSSVGAPQLVQSGQIDYLVFDYLAELT
                     MSILAAARLKKPELGYATDFVSVAMKSVLKDVVAQGIRVVSNAGGVNPEGCADALRAL
                     AAELGVQVKIATVTGDDVMPLLPALREQQPPVAELQSGAPLPARVVSANAYLGALPIK
                     AALDAGAQIVITGRCVDSAVTLGVLMHEFGWQATDYDRLAGASLAGHIIECGCQGTGG
                     LHTDWQAVPDWAHIGYPIVECAQDGSFSVTKPAGTGGLIAPQVVAEQLLYEIHDPARY
                     LLPDVVCDFTQVRLTQTGPERVQVEGARGLPPPASYKVSATYVDGFKTSAQLTIVGFD
                     AVAKARRTGEAILERVGELLQQQGLGPFSATHLEVLGAESPYGPHAQPAAAQMREAIL
                     RLTARHPAKAALELLAREIAPSGTSFAPGTTGAGGRASVSPLIRQYAFLLDKARIAPQ
                     VTLDGAAVSVPNQPPAPVEQAPEAIESIVIQEAYALPAGVESVEVPLLRLAWARSGDK
                     GDASNIGVIARKPEWLPLLRAQLTEARVKDWLAHLVQGPVTRYEVPGIHAFNFVCQQA
                     LGGGGMASLRNDALGKGMGQILLAMPVRVPMSWVN"
     gene            complement(12597..13376)
                     /locus_tag="PKN51_02865"
     CDS             complement(12597..13376)
                     /locus_tag="PKN51_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT84070.1"
                     /translation="MTSSPEPELLSRQHDGVLWLTINRPERRNAISPAVLAGLSEALG
                     RANQDREVRAIVLTGVGDKAFCAGADLQTGQSFKFDYSEPTQGMANLFRLTRQLNVPL
                     IARVNGACMAGGMGLMAMCDMAVAAPHAVFGLPEVKVGVFPAQVLSVLSGLIGPRALT
                     ELCITGEPITAAQALQLGLINHVSDDLDAGVQALLGRFLDKSPAAIRRGLYLMKRIGT
                     MSFEQSMAFAESQIALFALTKDAAEGQAAFREKRKPRWSGK"
     gene            complement(13397..14647)
                     /locus_tag="PKN51_02870"
     CDS             complement(13397..14647)
                     /locus_tag="PKN51_02870"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNT84071.1"
                     /translation="MTPQAANPTQPAGPLAGVRVLDLTTVFMGPSCTQLLGDLGADVI
                     KIEAPSGDSTRSIGPCGDIGLGPLFLGLNRNKRSVVLDLKQPAAVAALLQLARDADVF
                     ATNVRPAAMRRLGLGPDVLLAANPRLIYASMVGFSQRGPYAAKAAFDDMIQAATGLAA
                     VVGEAAGEVPRYLPITIADRSVGLYAFGVISAALYARERTGRGQSVEIPMFETMIPYV
                     LGDHLYGRTFEPARGDFSYPRLMSPNRRPYKTRDGYVCCLIYTDRHWELFLNAIGKGE
                     LLKTDPRLANIRTRTQAIDALYQLVADELAQRSTAEWRALLPESEIPIFPMHTFESLL
                     HDEHLQQTGFFQHTEHPVVGAVLETAVPSEWSDTVPRHQRPVPALGQHTTEVLREAGF
                     APADIDALLASGAARAAKPPSSTA"
     gene            complement(14644..15618)
                     /locus_tag="PKN51_02875"
     CDS             complement(14644..15618)
                     /locus_tag="PKN51_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT84072.1"
                     /translation="MSFRPIHTLLALTAAATTLLAQPAAAQDFPSKPITLVVPFGPGS
                     GTDQMARLYAKALGDEAKVSVVVDNKGGASGFIAAQHVAKAAPDGYTLMMTTNTTHAA
                     NEHLFKKLPYDPVKDFAPIGLLSTGQMLLLVRPDSPYKSLADLLAAAKKAPGKIDFGS
                     GSSSSQVAGYLLQQMAHVDMLNVPYKSNPQAITDLIGGRFAFMFADAPTALPQVQGGK
                     LRALAASSGKRLASLPDVPTVAEAGVKGYDMSYWFAAYAPAGTPPAVIAKLNQLFAKA
                     SATEEVKASLARTSGVLALGTPEGLAQFQAAESKKWGEVIRAAGIEAQ"
     gene            complement(15615..16802)
                     /locus_tag="PKN51_02880"
     CDS             complement(15615..16802)
                     /locus_tag="PKN51_02880"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNT84073.1"
                     /translation="MSNATAMGVLAGVRVLELGQVLAGPFAGAIFADLGAEVVKVERV
                     EGGDDARAMGPAFRHGDALNFHIFNRGKQSVALDLKSADGLAAFERLVGEADIFIHNL
                     RPGVPAKLGIDGASLCARHPRLIYCEISAFGSTGPMAMQPGYEPLVQAFSGLSSINGG
                     PDDPPMRSGASMCDQGSGMWAVIGALALLQRRQQTGRGGILQTSLLETALVWNGQKAD
                     AYVNEGRLPLRDRSGHPGFVPYEAFDTQDAPLLICCGNDRLFAKLATELGRPDWLADE
                     RFASNRARLAHKAELLAQLQPLLRARPRAEWAARFEAAGVPCAPIHSVPEALAHPQVQ
                     ALGILAPLPGEDFALTALPFSIDGARPLPRSAAPRLGADNAAHGLPPATVTPFTQNLQ
                     ETP"
     gene            16900..17811
                     /locus_tag="PKN51_02885"
     CDS             16900..17811
                     /locus_tag="PKN51_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT84074.1"
                     /translation="MRLDLSLRQLEAFVQVADSGSFRRAAARLGQAQPVLSRLIGQAE
                     QTLGARLFDRDTRRVEITPAGRELLPLAQRLLRDFDDTLGDFGAFMAGRSGRVSMVVL
                     PSVSVAWVPRAMAAFARTHPQVNFALTEAPADTLLALLEAGRADFGISVRPAPQQRLH
                     YRHLQDDPFVLVCRRDDPLAARAAVPWSVFGMRDCIMSAAHSSIRQVTDAVFLRQRTP
                     PSPVLEYPSVAAAGALVAAGLGITALPVLALQLVDMRELVAVPLVRPAMARPIGIVTR
                     IGRSLSPATQAFMASLQAAPAGAPDAP"
     gene            complement(17762..19087)
                     /locus_tag="PKN51_02890"
     CDS             complement(17762..19087)
                     /locus_tag="PKN51_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNT84075.1"
                     /translation="MNALTLFLPCAGGVEDFLADEVHGLSGLVGEDLLSLRGGVRVRG
                     DWALMQRLNLHSRLAQRVLIELAHGPYQSEQDLYALAAGVPWEQWFTPRQRFRVDLTA
                     QASPLKSLNFATLRIKDAVADRFRAQAGGLRPSIDTQRPDVRVQAHLTATHAQLYIDT
                     SGEPLFKRGWRQDKGDAPLKETLAAAMLAVCGWWQPATRSVSPLPLYDPCCGSGTIVI
                     EAAQLLLGLPAGGQRRFAFEQLRPHDPAGWNAIKTEAAGACSTSAGDQNDAQTPAVRI
                     FGSDVAHRMVDFAQRNARRAGVAAAVQLRGGDALQRLPPCAEPGVLLLNPPYGERIAA
                     AGVAGARGGREAFQSGTLARQRAGGAAPADDVNAGDDFFARLAAHWKSHYAGWSAWLL
                     TPDSKLPGRLRLKESRRVPLWNGPIECRLLRFDLTARRAPPPAPPAATP"
     gene            complement(19084..19530)
                     /locus_tag="PKN51_02895"
     CDS             complement(19084..19530)
                     /locus_tag="PKN51_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT84076.1"
                     /translation="MARIVFDLPAQFGFATELQIYISHVNQGGHLDNAQLLALVSEAR
                     LRFFHALGYREGHVDGAAIVVGDMLAQYKSEAFHGETLRVQMQPADFNRYGFDLVFRM
                     TEVSQGREVARGKTGIVFIDPVSRRVTPIPAPIQGKLLACAAAATP"
     gene            complement(19545..21092)
                     /gene="rpoN"
                     /locus_tag="PKN51_02900"
     CDS             complement(19545..21092)
                     /gene="rpoN"
                     /locus_tag="PKN51_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNT84077.1"
                     /translation="MKPGLSLRVAQQLALTPQLQQSIRLLQLSTLELSGEVEQMLADN
                     PFLERDDEAGDAAAPTPADRGERTDENAIDSGAAGADSTSAEADFDAEPLTPDDWDGD
                     GSVDIAPDDAEWGGEAPARQREAGDDDTDPGERVGGQESLAEHLLRQALTLRLAPEDA
                     AALRFLIGSLNDDGYLEDSLAQLAASLTEDGDELEQMQELVHRLTLALKLLQSLEPAG
                     VGARHLAECLTLQLRSRQAERALAPQEQARIATALRICARHDAVELLARRDSRKLAQL
                     VGESEDAVRAAMALIGRLDPKPGRRFADVERQIVVPDVIVSAVGTAERPRFRVALNPD
                     VVPRLRVHEQYASALRGSDKHPALSERLQEARWFIRNIQQRFDTILRVANAIVLRQRD
                     FFIHGELAMRPLIQRELADELGVHESTISRVTTAKYMATPRGTFELKYFFGSALGTES
                     GSHASSTAVRALIRQLIEAEDRARPLSDAQIAEQLKAQGIDCARRTVAKYREALRLPT
                     ASLRKSH"
     gene            complement(21089..21883)
                     /gene="lptB"
                     /locus_tag="PKN51_02905"
     CDS             complement(21089..21883)
                     /gene="lptB"
                     /locus_tag="PKN51_02905"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNT84078.1"
                     /translation="MASRFDAPAAADAPRAHGQSRLQVRGLRKNYGARRVVHDVSLDV
                     VNGEVVGLLGPNGAGKTTSFYMIVGLVRADAGSIAIDDQPIEHMPIHRRSRLGLSYLP
                     QEASIFRKLTVAENVRAVLELQVDAQGRPLGRDAIEQRLNELLQQLHVEPLRDSPAPA
                     LSGGERRRVEIARALATQPRFILLDEPFAGIDPIAVIEIQRIIAFLKSQGIGVLITDH
                     NVRETLGICDHAVIISEGRVLATGTPADIVANPEVRRVYLGEHFRL"
     gene            complement(21916..22557)
                     /gene="lptA"
                     /locus_tag="PKN51_02910"
     CDS             complement(21916..22557)
                     /gene="lptA"
                     /locus_tag="PKN51_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNT84079.1"
                     /translation="MKSSVPPFAPSLRLLCATLLLGLAAPAWAERADRDKPMNIEADA
                     LRYEEQKQQSTFTGKVLVTKGTIVMRGARLEVRQDSAGNQFGTLSGEPGKRAFFRQKR
                     EGVNEFIEGEGETIEYDGKTDTVRLIRRAEMRRLEGATQLDQVLGSLIVYNNRTEVYT
                     VDGAPAAGAPSAAPGGRVRATLAPRQAASAASAASAPALQLRPAAALPEGSGQ"
     gene            complement(22684..23313)
                     /locus_tag="PKN51_02915"
     CDS             complement(22684..23313)
                     /locus_tag="PKN51_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNT84080.1"
                     /translation="MNRREFTLAGALALPGLLALPARAQSGPGYATLKDRAPVDAPAG
                     QIEVIEFFSYGCSHCKNFEPVFEAWKKAAPKDVTVRREHVGFQSSFEPLQRIYYALDA
                     LGLTEQPVHMKVFQALQTERKRLDKPDVLLPWIAEQGVDRAKFEAAYKSFGIANKLRR
                     AVQLQEAYHVEGTPALGIAGRYYTDGSMAGGFERMIQIANNLIAQERKK"
     gene            complement(23382..24116)
                     /locus_tag="PKN51_02920"
     CDS             complement(23382..24116)
                     /locus_tag="PKN51_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNT84081.1"
                     /translation="MICKQRGGTFLGLLLGVLVGLAAALAVAIYVAKVPVPLVSKNAT
                     RTNGQDAAEAEKNKDWDPNAALRGKNGTRPAGPGPAASGSVVPAPAPAPIPAPTEVAR
                     PAPDSKPADARAPARGADKTAEPAPPAVTARPAPARPSSDPLGDLAAARSGAAPAAPA
                     AAAAADPFAYFVQAGAFRTPEDADAQRARLSLMGVEARVSEREQAGRTVYRVRVGPFQ
                     NRDAADRVKQRLDGEGFDAALVRVQK"
     gene            complement(24136..25809)
                     /gene="argS"
                     /locus_tag="PKN51_02925"
     CDS             complement(24136..25809)
                     /gene="argS"
                     /locus_tag="PKN51_02925"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNT84082.1"
                     /translation="MQSVKTELLAALAAELERLWPGSGSKAAFESPRQAAHGDFACTA
                     AMQLAKPLKANPRALGEQLQQALQAAPAFQRWVSAIELAGPGFLNIRLQPAAKQQVVL
                     EVLSQGARYGSRPARGEKMLVEFVSANPTGPLHVGHGRQAALGDAICHLFTSQGWQVQ
                     REFYYNDAGVQIDTLATSVQCRARGLKPGDADWPEAAYNGDYIQDIADDFLARRTVQA
                     DDRQFTASGSVDDLDGMRQFAVAYLRHEQDKDLQAFRLKFDRYYLESSLYAEGRVEAT
                     VQRLIDAGKTYEEGGALWLRSTDYGDDKDRVMRKQDGGYTYFVPDVAYHITKWERGFR
                     KAINIQGTDHHGTIARVRAGLQAAGVGIPAGYPDYVLHTMVRVVRGGQEVKISKRAGS
                     YVTLRDLIDWTSADAVRFFLLSRKPDTEYTFDVDLAVQQNNDNPVYYVQYAHARICSV
                     LQAWGGDAATLADADLTPLDTPAAQALMLQLARYPDMLSAAAQDFAPHDVTFYLRELA
                     AAYHSYYDAERILVDDPAVKTARLALVAATAQVLHNGLTVLGVTAPTRM"
     gene            complement(25941..26390)
                     /locus_tag="PKN51_02930"
     CDS             complement(25941..26390)
                     /locus_tag="PKN51_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HNT84083.1"
                     /translation="MLEAWLAFFHISAVIGWVVFASAQAAICRAEWLNATVVQRLARL
                     DRILWIATAAVLLSGLARICWGAKGAAWYWSNPLLHLKLTLFLVAAVLMLGPTRRIAR
                     WQRALAADASLPAAHEVQGVRRLVMIETHLVAVIPLAAVFLARGYGA"
     gene            complement(26421..27410)
                     /locus_tag="PKN51_02935"
     CDS             complement(26421..27410)
                     /locus_tag="PKN51_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT84084.1"
                     /translation="MDKLKAFETFASVATRGSLSAAARAEGVAPAVVSRRLDALEEHL
                     GVKLLVRTTRRIALTHEGTAFLDDCQRILAEVAGAEASVSAGGVQASGHLRVTAPAGF
                     GRRHVAPLVPEFHALHPRLRISLNLSDRVVDLAAEGYDCAVRVGDLPDSSLISIRLAD
                     NQRLCVAAPAYLARRGRPRHPRELAQHDCLMLSSDASQSRGWAFRVPRQAHGQNDERP
                     RAGAGVPIQRSDVEYDVIHLRPEGRLDCTDGQVLHDWCLAGHGIAWRSTWEVEHAIAS
                     GRLVPVLEAFAAPPNGIYAIVPHPTRHLPLRVRLWVDHLRQRYGDPAFWRRPG"
     gene            27521..28219
                     /locus_tag="PKN51_02940"
     CDS             27521..28219
                     /locus_tag="PKN51_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84085.1"
                     /translation="MRAESAPPIDRRHIDVRTMSLAQLQKLAERGSRRARAELEARMR
                     APGPVLLPASVPADRTAPSAPSRHPPTLTARADPPPQATAAGAAAPAPGAVPAGQGVP
                     VSADALAQQLHLIARQDEAHARAAGPPRLVGLVLTAWGVLLLLGGLLMLGRGGAALYY
                     LFCGLGAAAVGALLMRRSRWALALHGALLPLALGWAWAGAGRHSLPLSVVQAGPLLIP
                     ALWMALRQVREALE"
     gene            28471..30690
                     /locus_tag="PKN51_02945"
     CDS             28471..30690
                     /locus_tag="PKN51_02945"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HNT84086.1"
                     /translation="MTARTRIHDLHVADTLYEFINREVLPDVGVDAARFWQGFSAIVT
                     ELAPKNIALLAERDRLQKELDTWHQANPGPIKDMRAYRAFLERIGYLVPQPAKVKVTT
                     KRVDAELAVQAGPQLVVPILNARYALNAANARWGSLYDALYGTDVIAETRGLEKGAKY
                     NPRRGAKVIEWARHVLDRTAPLKKGSHVDSVAYLVRDGELVVKLKDGKSSKLADPAQF
                     IGYQGEPKAPSSILLLHHGLHLDIRIDPASPIGRNDPAGVADLVVEAAVSTILDLEDS
                     IAAVDAEDKVLGYRNWLGILRGTLTESFDKGGKTLTRGLNPDRLYTAASGKGQVRLHG
                     RSLMFVRNVGHLMTNPAILYRAADGSQREIPEGIMDAVVTTTIALHDLQRKGANGIRN
                     SRTGSVYIVKPKMHGPAEVAFAAELFGRVEQLLGLADSTVKLGIMDEERRTSVNLKAC
                     IAAAKSRVAFINTGFLDRTGDEMHSCMRAGPVYRKSDMKTSAWIQAYERNNVLVGLAC
                     GLRGKAQIGKGMWAMPDLMKAMLEQKIAHPRAGANTAWVPSPTGATLHAMHYHQVLVS
                     EVQQRLEAELAQADPAALRDELLTGLLTIPVVSEDKWTAAEKQQELDNNAQGILGYVV
                     RWVDQGVGCSKVPDIHNVGLMEDRATLRISSQHMANWLLHGVVSEGQVRATLQRMAAL
                     VDQQNGGDPLYRRMHDHFEQSAAFQAACDLVFKGVQQPSGYTEPLLHAWRQKVKAGA"
     gene            complement(30808..31191)
                     /locus_tag="PKN51_02950"
     CDS             complement(30808..31191)
                     /locus_tag="PKN51_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNT84087.1"
                     /translation="MSQTLTPAQLEQLKQRLLARRQELYAQMQQNRANLAPAENTAGS
                     VSQDENARLANQTREVDTRLTALDAEELARIDRALEAMDAGDYGRCDECGCQIPFERL
                     QIEPMTQHCVACKSAWEHKQGLARA"
     gene            31270..31485
                     /locus_tag="PKN51_02955"
     CDS             31270..31485
                     /locus_tag="PKN51_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HNT84088.1"
                     /translation="MPLSAPPIDPTRCPLCGQPNRCAQQARPAGAPAAPHCWCMERRV
                     APDALARVPAQARGRACLCPSCATPRA"
     gene            31500..32969
                     /locus_tag="PKN51_02960"
     CDS             31500..32969
                     /locus_tag="PKN51_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT84089.1"
                     /translation="MHDPPPSSSAEALRARHGARWRWLLLLTVMVGTMASIMSSTVVN
                     VAIPGLSQQFTLGQARAQWVSSGFMVAMTVSMLTTPWLLARFGYRATYNGCMLLLLAG
                     GVLGGVAQSYPLVLAARVAEGLAAGVVQPIPAVIIMRAFEPNEQGRASGLFGMGVVLA
                     PALGPSIGGVLVDWFGWRSIFFMVAPFCLLALWLARRYVPTTAPGGAATRRDETLDGV
                     GLLLGSAGTLCLLNGLVALRAGPAVEAAALLALAAICLAGFIGWQRRCVRLGRAPLMN
                     LALFAWRPFAMGVLVALIYGTALFGSTYLLPVYMQMGLGLSASSVGAILLPAGLVLAA
                     TIPLVARWADRLPASGLVNTGLALLALSFAAMVTVGLAPGLWHLTALGIVGRIGLGFI
                     LPSLNLGAMRGLALPLIPQGSSVINFARTLGGAAGISLCGIALEWRLAAHGTSLTEGA
                     PSAARLAAFDEVFLMLAALCGMAMLAARHLSTPAAGADR"
     gene            32980..33747
                     /locus_tag="PKN51_02965"
     CDS             32980..33747
                     /locus_tag="PKN51_02965"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNT84090.1"
                     /translation="MCQLLGMNCNTPTDVSFSFSGFAQRGGRTDHHADGWGIAFFEGR
                     GARVFLDPGAAADSPVAALIRSYPIKSRNVISHIRKATVGAVTLENCHPFVRELWGRY
                     WVFAHNGDLKDYAPRLHGHFHPVGSTDSEHAFCWLLQELAKAHADVPPVAELTRTLRE
                     LLPQVARHGSFNLLLSNGEALWAHASTHLSYIERRHPFASARLADEDLSINFADCTTP
                     SDRVAVIATQPLTCDERWTAFEPGSLKVFVDGALQNL"
     gene            complement(33822..>34887)
                     /locus_tag="PKN51_02970"
     CDS             complement(33822..>34887)
                     /locus_tag="PKN51_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT84091.1"
                     /translation="NAVLSVAAAIVISRWRRPHRPHPLGRESLRGAMRVGLQYVMQSY
                     HLKGVLLRVALFFFHSTALIALLPLVARQIEGGHAGTFTVLLAAMGAGAIGSTFVLPR
                     WRQMFTRDRLVLAGACTQALMMALMAFTHSLWLAVPAMLLCGVAWLTTANSLSVSAQI
                     SLPDWVRARGMSMYQMAIMGATAVGAAVWGQVATWSSVPVTLGIGAASGIASMALATW
                     RWPDRGSIDDPTPAGPWPLPQAEAPPPHGHVIVTVEYQIDPAQAPAFRALMEESRRSR
                     LRVGAVGWELLSDINDPGRFIEVIEDASWTEHLRRFERVSVQDAALRERKLAFHIGGQ
                     PPIVRRAVRESTVRGERPST"
BASE COUNT         4959 a        12172 c        12456 g         5300 t
ORIGIN      
        1 gagcagtctg gtagctcgtt gggctcataa cccaaaggtc ggaggttcaa atcctcctcg
       61 cgcaaccagg ataccccggt caaaaatccc gctccgtgcg ggatttttgc tttccggccc
      121 cgctgttgtg taccgcacca tgaccgccac caccctttcc gcgctgcgtt acctgaccgt
      181 accggtgacg ccgtttgcgc agaactgctc catcgtctgg tgcgagcgca gcaggcaggc
      241 ggcgatcatc gatccgggtg gggatctgcc gcgcatcgaa gccgcgctgc gcgagctggc
      301 ggtgaccccg acccagatct ggctcaccca cgcccacatc gaccacgccg gaggcagcgc
      361 cgaactgagc gccaggctgg gcctgccgat cgtcggtccg cacccgggcg accaattctg
      421 gatcgacggt ctggccgagc agggccgcat gttcggcttt gcgcgcgccg agccgttcac
      481 gcccacgcgc tggctgcacg atggcgacac ggtgcgggtc ggcgactgcg agctgcaggt
      541 gcgccactgc cctggccaca ccccggggca cgtggtgttc cattcgcccg aggcgcgccg
      601 cgccttcgtc ggcgacgtac tgtttgccgg cagcatcggg cgcaccgact ttccgggcgg
      661 caaccacgcc cagctgatcg ccagcatccg cgagcgcctg tggcccatgg gcgacgacac
      721 cgtgttcatc cccggccacg ggccggagag cacgtttggc gaggaacggc gccacaaccc
      781 gttcgtgcgc gatggcctct gaggcgccgg cggcagggcg gccgcggcgc gactggcgcg
      841 ccagcctgcg catctacctg tcgccgccca gcctgcgcat gctggcgctg ggctttgccg
      901 ccggactgcc gctgctgctg gtgctgggca cgttgagctt tcgcctgcgc gaggccggca
      961 tcgaccgctc caccatcggc tacctcagct gggtggggct ggcctatggc ttcaagtggg
     1021 cctgggcgcc cctggtggac cgcgtgccgc tgcccgggct gacgcgcctg ctggggcgcc
     1081 ggcgcgcctg gctgctgctg gcccagctcg gcgtgatggc cgggctggtg gccatgggct
     1141 gggccgatcc gcgcgtgtcg ctggccgccg tcgtgtggag cgcgctggcc gtggccttcg
     1201 cctcggccac gcaggacatc gcgctggacg cctaccgcat cgaatcggcg cccaccgatg
     1261 cgcagccggc gctggcggcc agctaccaga ccggctaccg gctggccatg atctgggccg
     1321 gcgccggcgt gctgtggctg gcggcgcgcg cgcagggcga cgccagcggc taccagcacg
     1381 aggcctggcg gcaggcctac ctggtgatgg ccgcgtccat gctgccgggg ctgctgacgc
     1441 tgctgcgctc gcccgagccg gcgccgcaac cgctggcgcc ggcgcgcagc gtgggcgagt
     1501 ggctgcgcac cgcgctgatc gagccgttcg ccgacttcct gcgccgctac cgctggcagg
     1561 cggccctgat cctggcgctg atcgccgtct accgcatcag cgacgtcgtg atgggcatca
     1621 tggccaaccc gttctacgtc gacatgggct acaccaagga cgaagtggcg gcggtgacca
     1681 aggtctacgg cgtggcgatg accctgctgg gggcctttgt cggcggttcg ctggcgctgc
     1741 gcttcggggt catgcgcatc ctgatgctgg gggcgctgct gtcggcgctg accaacctgc
     1801 tgttcgcctg gctggccggc catgggcaca gcctgccggc gctgatcgcc gtggtgtcgg
     1861 ccgacaacct ggcctcgggc atggcctcgg cggcgttcat cggttacctg tcgagcctga
     1921 ccaacctcag ctactcggcc acgcagtacg cgctgttttc ctcgctgatg ctgatgctgc
     1981 ccaagttcat cgccggattt tcgggcgact acgtcaacgc cttcggctac gccggctttt
     2041 tcaccagcac cgcactgctg ggtctgccgg tgctgctgct ggtggcgctg gcggcgcggg
     2101 tgccgacccg tcatgcaggg caaaatgagc ctgtggcgca ggcgggtggt acgccataag
     2161 ctattgattt tgtagtatct ggggcgccgt gccggtgtgg cggcgcgcat cgacatggac
     2221 tccttcagca acctggccgt gctggcgctg gctgcctttg gcgccggtgt gctcaacgcc
     2281 atcgccggcg gcggcacctt cctgaccttc ccggcgctgg tgtggaccgg cgtgccgccg
     2341 atcgccgcca acgccaccag cgcgctggcc gtctgccccg gctacctggg cagtgcgctg
     2401 ggctttcggg ccgagctggc ggcgctgccg cgtgcgctgc ggctgcgcga ggtggcgctg
     2461 agcgcgctgg gcggcgtggc cggcgcactg ctgctgctgg tcacccccgc caaggtgttc
     2521 gccgccgtgg tgccctggct gctgctgttc gccaccgtgc tgttcgcact tgggccgcgg
     2581 ctggccgcct gggctggcgg cgaccatggc ggcggtctgg cgcgctggcg cctgccggcg
     2641 ctgctggcgg tggcgctgta cggcggctat ttcaacggcg ggctgggcat cctgctgatg
     2701 gcgctgtacc tgttggtggg cgaaacccgc atgaacaccg tcaacgcgct gaaaaacctc
     2761 aactccttcg tgctgtcggt agtgtcggtg gttgccttcg cgctgggcgg cgccatcgtg
     2821 tggcgccagg cgctgctgat ggcggtgttc gccaccctgg gtggttttgc cggcgcgcgg
     2881 ctggcgcggc ggctgccggt ggcctgggtg cgtgccatcg tgatcgtcac cggtctcgcg
     2941 atgagcgcgc tggttttcgc gcgcagttcc tgaaggcggc gcatcacggt ccgagagccc
     3001 gcagtacgtg ggacaggcca tgccagcgcc agccgtggcc cagactgatc cggccagcgg
     3061 ctgcgttcct ccgagacggc gaaacgtcgc gaagcgagtg gagtctgggt gtgggccgtc
     3121 tatcgtttga agcgaccatc gcggccgatg atggacagtt ccacgtagct cagccccgtg
     3181 tggtcggtgg cgctgaagcc cagctccagc ccgcccaggt cgaagcgctg caggccgttg
     3241 agcgcgtcga tgatcttctc gcgcgtgggc ttggggccgg cgcggcgcag cgcttcaacc
     3301 aacaccttgg cactggcaaa accctccatc acctgcggcg tcagctcccc cttgccggcc
     3361 agcagggcaa cggcttcgcg gttgatcggg atgcccagcg ctcgctcgct ggggaacacc
     3421 tgcgacacga caaccccacg cgcatgctcg ccgagctgct tgatgaagcc gcccgaggcg
     3481 ttgttggaaa ccgtcagcac ctgtgccgcc gatcctgcct ggcgcagcgc cttgacgcca
     3541 tcgctcactg ccacgctgga gccgatccag agcaccgcct gggcgttcgc gtccgtcagt
     3601 tgtttgacga tggccgggta gtcgggcttc tcgcggtcgg ccttgatgac ggccaccggc
     3661 tttttgccgg cacgttcaaa gcccttctgc gcgccttcca ggcagtcggc gccgaagctg
     3721 tcgtccacgt gcaccacggc cacccgctcc agcgtgacct ggaagacgtg ctcgatggca
     3781 cgctcggcct ccgcctggta gctggagcgc acgttgaaca catacggatt cacaggccgg
     3841 tgaaacacca tggcgccggt ggaaggcgcc accagcgcca gcttgtgctt ggccagcagt
     3901 ggcaggatgg cctgggtgtg cggcgtggcc cggctcatga acagtgccac cacgccctcc
     3961 tgctcgatca gcttgcgcgc gttttccgcc gccagcggcg gcttgaagtt gtcgtcgagc
     4021 gtgatgagct tgatcttctc gccatgcaca ccaccctggg cgttgacgtg gtccaggtag
     4081 gcgcgcacac ccagcagcgt ctcgcccacg ctggcggcca cggggccggt cacggcggtg
     4141 gtctggccga tcaggatgtc ggcctgtgcc gcgccgccca ggcacagcaa tgtcgtcagc
     4201 gccaacttcc gcagacggcc atgcatgccc ggagcgaatc cacgggacat caggatgggg
     4261 tcacgtttga gcgcagtgaa cataggcagc catgcaactg gaatagggga acgcaaccat
     4321 acgcacaatc ccttcacgct gtccacccgg tctaacccaa aatggacggc agtgcctgcg
     4381 aaaatggtca gatgcagacg aagcaacgca tcgtggtcgg cctgtccggc ggggtggatt
     4441 cggccgtcgc cgcctggctg ctgcgccagc agggccacga cgtggtcggc atcttcatga
     4501 agaactggga agacgacgac gacagcgagt actgctcgtc caacgtcgac ttcgtggacg
     4561 cggcggcggt ggccgatgtg atcggcatcg agctcgaaca cgtcaacttc gccgccgaat
     4621 acaaggaccg cgtgttcgcc gagttcctgc gcgagtacca ggccgggcgc acgcccaacc
     4681 cggacgtgct gtgcaacgcc gagatcaagt tcaaggcctt cctcgaccac gccatgcgcc
     4741 tgggcgccga aaagatcgcc accgggcatt acgcccgcgt ccgcttcaat gccgccaccg
     4801 gcctgcacga gctgctcaag gggctggatc cgtcgaagga tcagagctat ttcctgcacc
     4861 ggctgaacca ggcgcaactg gcgcgcaccc tgttcccggt gggcgagttg cacaagaccg
     4921 aggtgcgccg catcgccgcc gaaatcggcc tgcccaacgc gaagaagaag gactccaccg
     4981 gcatctgctt catcggcgag cggccgttcc gcgaatttct cgggcgctac ctggcccagc
     5041 agccggggcc gatcaaggac gagcgcgggc gcaccatcgg tcgccaccag ggcctgtcgt
     5101 tctacaccct cggccagcgc caggggctgg gcatcggcgg tgtcaaggac cgaggcgcgg
     5161 cgcgcggcgg cggcgaccac gcgccctggt tcgtggcgcg caaggacatg gcgcgacaca
     5221 ccctgtacgc cgtgcaaggc cacgaccacc cgtggctgct gtcgcgcgcg ctggtgttcg
     5281 acaacgccag ctggatcgcc ggccagccac ccgccgccgg cgcctgctcg gccaagaccc
     5341 gctaccgcca gcaggacgcc gcctgcaccc tgtcggccac tgcggtggcg ggcagctggc
     5401 gcctgcgctt cgccgagccg caatgggcgg tcactcccgg ccagagcgcg gtgctgtacg
     5461 acggcccggt gtgtctgggc ggcggggtca tcgcggcggc cgaagaatat gaacccgatg
     5521 cggccacagg gcttgcgcag caagcgccag aagctatgta aacaatagca ttaacgcagc
     5581 gacagcccgt aggccagcag aatcagcagc acgatcagcg tcagcgcacc gatcagcgcc
     5641 cgtcgccagc gcgccggggc gtgctgcagc tccatgaact gcaggatcac cgccaggccc
     5701 ttgagccagg ccagggcaaa caccagcgcc atgccggcca gccccgccga cgccacccag
     5761 ccgctgcggc ccagcaccca ggtgatggcg gtggccacca gcatggccag ccaggcaagg
     5821 tcgaggatcg ggcgtcctgg gggcatgtcg cttcgcgcct cagcgcatca catagaccag
     5881 cgggaacagc accagccaca gcaggtccac caggtgccag aaggcgccgg cggtctccag
     5941 cgtgtgcggc tgcagtcggc cgcgctggcg cgccagaccg acggcggtca gcgccagcgc
     6001 cagcaggccg gccagcacgt gcagcaggtg aaagccggtc aggatgtagt agaagtcgaa
     6061 gaacaggttg ccgtcgccca tgccgatgcc ctgcgccgcc ttgtcggcgt attcccgcag
     6121 cttgaagacc acgaagcctg cgcccagcag ccaacccagg cccagccagc ccagcgccgc
     6181 gcggcgcgca tcggcgcgcg tggcgtgcac cgcccgcgcc accgcccagc tggccgacag
     6241 cagcagcgcg gtgttgaggg cgcccgcgct gacgtccagc gcctgctgcg aggtgcgaaa
     6301 cagctccagg tgccacagcc gcgtgaaggc aaaggcgccg aacagcacgc agaaggtggc
     6361 cagctccagc gcgatcacgt accagatgaa ctggtcaccc ggcaggcgtg gcgcgggggc
     6421 gtggcgtggg gtcacagatg cttgcaaaat gatagctgct gacgcacgtc cgacgggcgt
     6481 cagaagcctg aatcttcatc caacggttca ctgcgcctgc ggtggcggcg cgctggcggt
     6541 cgcggccatc gcggccaccg gctcgggttg cagcaggctg ccctggcgcc agcccttcca
     6601 ggtggcgatg ccgagcacca acaccaccag cgacgccagc gccagcgcca ccagggccgg
     6661 cagctgggcc gcgccgtcct gctgcgccag ccgcagcgtc agcgcggcaa aggccgccag
     6721 cggaaagccg agcgaccaga aggcgatgga aaacggctgc gccagcatgc gccgcagcac
     6781 ctgaccgctc cacagcagaa agaacagcgc caccccccag cacatccagg ccagcgtcag
     6841 cggaccgccc agcagcagca cgccgctgcc gaccaccgac ggcggcgcca cgctgatgaa
     6901 ggtgacggcc agcagccgct ccggccacaa gccctgggtg gcgatgcgcg cgccgatcag
     6961 cgccagcagc accggccaca gcagcagccc gaggccgaac tgcgccgccg tccaggctgg
     7021 cgcgcccagc atcacgccgc ccagcggcgc cagcacgttg cccaccaccg ggatgaacag
     7081 caccggcgtc agcgccgccc agctcaggcc ctgggtcttg tcggcgctga gccagcgcgc
     7141 cagcacccac agcgtgacgc cgaactgcgt cagcgagccg agccaccaca gcaccgcgac
     7201 ccagctctcg ggaccgaaca gcatggccgc cgccgacgcc agcaggatca gcgacaccgg
     7261 cagcgtggcg atgaaggcgt ggcgcaccgg gtgcttcagg tcggccgcca gcgcctcggg
     7321 gtggtgctgc cagcggatcc acgacagcac cagcagcgcc acgaacagca gcgccgccgc
     7381 cgccgccagc accagcgcgc cggcggtggc cagctcaccc agcagcggcg tggcgcgcgc
     7441 ccaggcgatg gccaggccgg ccacgctcat caccatggca aaccagttgg gggcgaggta
     7501 cttcaaggcg tcgggtcgct gtggcatgcg gcgatgatgc cacggcccgc accggcaggg
     7561 cgctggtgct cagcggtgcg ggcattgcat gggtgcgtca gggcgtcatc gggtctgcct
     7621 gccgggcggc tgcaaaccga cctgattcca cacgcaacat ggtccaaccc tcaacaacgc
     7681 atgaacctgt ggtctgtcgc tcagttgcag ccaagtgatg cgccccatcg ggttgcatca
     7741 ctgagcccgt tcatggcgcc acgcgccgca gacgccgacc cgccagcagc gcggccactg
     7801 tggccagcag ggcgagcccc cattgaccga gcgtaggcac ggccgtcgcc agcagcgggg
     7861 tgatgacgac ctgtccatgg gtccccgtga ctgcggcagt accagatgcc aagccgccgg
     7921 caccacctgc gccaccggca ctgctggtgg tcaggccttg caccgctccg agatggcacg
     7981 aaccgcctgc accaccaggg ccgccatggg cgatgccgtc ctgcccggct gtgcctgcgc
     8041 ttccggaagt gacgccgccg ccaccaccgc cgccaccacc accatctccc ccaccggggc
     8101 tgccaccggt gccgccaacc gttcctgtgg cgcagttggc atcgctgggc gccgagccac
     8161 cagctgacgc ccctgaggca gcagtgccac cgagcgatcc gctgccgccg ccgccaccgc
     8221 caccccccgc ggcctgaagc gcgagagcgc ccactaccag gctgctgctg ccacccccgc
     8281 caccgcctcc accactgccc ccggtgctgc ccggcgcgcc gccggcgcct cctgaaccca
     8341 atcccgcacc cgcgttgcca ccagcggccg gattgccatc agagtcggaa gtgccgccgc
     8401 cacctccacc acccacggtg atctgcaccg tgctgccggg cgtcacaggg agcctgccct
     8461 gcagcaatgc accagagccg ccgccaccgc cgttcccggt ttgcaagagg tcgtagccac
     8521 caccaccacc accgccaccg gccaccgtga cctgcaaggc agtgacgccc gccggcacga
     8581 tgtaggtgta gttgccgggt gtggcgtagg tctgtgtctg ggccagcgca ccgcccgttc
     8641 caagactgca caacatcagc atcgaaatgc gcggccagcg cgacgtgaga caactcatct
     8701 tcttctcctc tccgcagtag tgaggggttg ggagacccca gtggtttcaa aagggaatgt
     8761 cgtcgtccat gtcatcgaag cccgacgacg gccgcgcggc aggggccgcc gcgcgcgggg
     8821 cgggggcggc gcgcggtgcc ggtgcgctgc ggcgcggggc gtcgtagccg tcgccaccac
     8881 cgtcgtccga cgggccgccc atgccctgac ggctgcccag catctgcatc tggtcgacgc
     8941 gaatttcggt gctgtagcgg tcctggccgg tggcctggtc ctgccacttg cgggtgcgga
     9001 tgctgccttc gacgtagatc tggctgccct tgcgcaggta ctgaccggcg atttcggcca
     9061 gccggccgtt gaacaccagg cggtgccatt cggtgtgttc gcgcggctcg ccggactgct
     9121 tgtccttcca cttgtcggtg gtggccagcg tcacgttggc cacctgatcg ccgttgggga
     9181 aagagcgcat ttcggggtcg cgccccaggt tgccgacgag gatgactttg ttgacggagg
     9241 ccatggcgtt gttcctgtga agtggaatgg gaaacagcgg gtgatcgagc ccgtgattgt
     9301 gccccatgca ccgccgccgg gccggcgcgg cgcgctcggg cgctgcatgc ctggccagaa
     9361 cccggcggtc acgcctcgac ggtcacaccc agcggcgtca gccggcgcgc cagggtgatg
     9421 atctgccggt ccctgctgag cagccgggcg cgccaggtca cggccaggtc gatgaagatc
     9481 tggtcgtcgg ggtcgcggca gcgcaccggt gcgggcggcg ccgcaggcac ccgttgcacc
     9541 cagcggtcga acgccgccag cacggcgtcg gcgctgcgcc ggtggcgcag cagctgccgt
     9601 gccacggcgg ggtaggtcag cacccgggcc agttcgaccc gcatggccgg ctgcgccatc
     9661 cagtgcagcc ggcccgcttc gatggcctga cgcaggggcg ccacctcggg atccccgaac
     9721 agccacagat cgagcacccg gttggtgtcg atcaccaccg gtggcggttc gggcggtggc
     9781 gcggccgggg tcaattgatg gtcagcttga ggctgcgcac gaagggcacc cagcgctcgc
     9841 gttccttgcg accgaaggcg tcggcctggg ccggggtgga ggggcccagg ttgtccgcgc
     9901 cgccgcgcgc cagccgctcc ttcatcgccg gcagagccag ggcggcgttg agcgcctggt
     9961 tcaggcgcgt gatgtcggcg tcggccacct tggccggggc gaacaggcag ttccaggtgt
    10021 aggcgtcgta gtcgcgcagg ccctgttcgg caaaggtggg cacgtcgggg atggccgcgt
    10081 tgcgctgctt gccgccaaag gccagcaggc gcacgcggcc ctgctgggcc atgggctggg
    10141 cggtgatgat gccgtcgaac accatgtcca catggccgcc ggccagatcg gccatggccg
    10201 gtccgttgcc cttgtagggc acgtggttga ggcggatgtc ggcggcgcgc gcaaaggctt
    10261 cgcccacgat gtggctgatc gatccgatgc cgcccgagcc gtagctgagc tggcccgggt
    10321 tggcgcgcgc gttggcgatc agctccttga gcgtcctggc gcccgcggcc ttgccgacca
    10381 gcaccagggg cgtgcggccg accaggccga tctggcgcag atcgcgctcg acatcgaacg
    10441 gcaggttctg atagagcgcc acattcatca ccatctgtga gctggtgccc aggccgaggg
    10501 tcaggccgtc gggcgccgcc ttggccaccg cctcggtgcc cagcgaaccg ccagcgcccg
    10561 gcttgttctc gacgatcact ggctgcttca gcgactcggc catgtggcgc gccagatcgc
    10621 gcgcgatcag atcgttggcg ctgccgggcg caaagccgac gatgatgcgg atcgggcggt
    10681 cctgctgggc ccagaccgcc ggggcggcga cgctggcggc cagcgccgcc aggctggcgc
    10741 ggcgcgagcg ggaaaagttg tggatcgggg gcatgcttgt ctccttgctt gtgttcagtt
    10801 cacccagctc atgggcacgc gcaccggcat ggccagcagg atctgcccca tgcccttgcc
    10861 cagcgcgtcg ttgcgcagcg aggccatgcc gccgccgccc agcgcctgct ggcagacgaa
    10921 gttgaaggcg tggatgccgg gcacttcata gcgcgtcacc ggcccctgca ccaggtgcgc
    10981 cagccagtcc ttgacccgcg cttcggtcag ctgcgcgcgc agcagcggca gccattcggg
    11041 cttgcgggcg atgacgccga tgttggaggc gtcgcccttg tcgccagagc gcgcccaggc
    11101 caggcgcagc agcggcacct cgacgctttc tacgccggca ggcagtgcgt aggcctcctg
    11161 aataactatt gattcgatag cttctggcgc ttgttccacg ggcgctggag gctgattggg
    11221 cactgaaacc gcggccccgt cgagcgtcac ctggggcgcg atgcgcgcct tgtcgagcag
    11281 gaaggcgtac tgccggatca gcggcgacac gctggcgcgc ccgccggcgc cggtggtgcc
    11341 gggcgcaaac gaggtgcccg acggcgcgat ctcgcgcgcc agcagctcca gcgccgcctt
    11401 ggcggggtgg cgcgcggtca ggcgcaggat ggcttcgcgc atctgcgccg ccgccggctg
    11461 ggcgtgcggg ccataggggc tttccgcgcc cagcacctcc aggtgggtgg cgctgaaggg
    11521 gcccaggccc tgctgctgca gcagctcgcc cacgcgctcg aggatggcct cgccggtgcg
    11581 gcgcgccttg gccacggcgt cgaagccgac gatggtcagc tgcgccgagg tcttgaagcc
    11641 gtccacatag gtggcgctga ccttgtagct ggctggcggc ggcaggccgc gcgcaccttc
    11701 aacctgtacc cgctcggggc cggtctgggt cagccgcacc tgggtgaagt cgcacaccac
    11761 gtcgggcagc aggtagcggg ccgggtcgtg gatttcgtac agcagctgct cggccaccac
    11821 ctgcggcgca atcaggccgc cggtgccggc gggtttggtg acgctgaagc tgccgtcttg
    11881 cgcgcactcg acgatggggt agccgatgtg ggcccagtcg ggcacggcct gccagtcggt
    11941 gtgcagcccg cccgtgccct ggcagccgca ttcgatgatg tggcccgcca ggctggcgcc
    12001 ggccagccgg tcgtagtccg tggcctgcca gccgaactcg tgcatcagca cgcccagcgt
    12061 caccgccgaa tcgacgcagc ggccggtgat gacgatctgc gcgccggcgt ccagcgccgc
    12121 cttgatcggc agcgcgccga ggtaggcgtt ggcgctcacc accctggccg gcagcggcgc
    12181 gccgctttgc agctcggcca ccggcggctg ctgctcgcgc agcgcgggca gcaggggcat
    12241 gacgtcgtca cccgtcacgg tggcaatctt cacctgcacg cccagctcgg cggccaaggc
    12301 gcgcagcgcg tcggcgcagc cctcggggtt gacgccgccg gcgttgctga ccacgcggat
    12361 gccctgggcc accacgtcct tcagcacgct cttcatggcc acgctgacga agtcggtggc
    12421 gtagcccagc tcgggcttct tcaggcgcgc ggcggccagg atcgacatgg tcagctcggc
    12481 caggtagtcg aacaccagat agtcgatctg gccgctttgc accagctgcg gcgcgcccac
    12541 gctggagtcg ccccagaagc cgctggcgcc gccgatgcga atgatttttc cggtgttcat
    12601 ttgccgctcc aacggggttt gcgtttttcg cgaaaggcgg cctgtccctc ggccgcgtcc
    12661 ttggtcaggg cgaacagggc gatctggctt tcggcgaagg ccatcgactg ctcgaacgac
    12721 atggtgccga tgcgcttcat caggtacagc ccgcgccgga tggcggccgg cgatttatcc
    12781 agaaagcggc ccagcagcgc ctgcacgccg gcgtccagat cgtcactgac gtggttgatc
    12841 aggcccagct gcagggcctg cgcggctgtg atgggctcgc cggtgatgca cagctcggtc
    12901 agggcgcgcg gcccgatcag gccgctgagc accgacagca cctgggccgg aaacacaccc
    12961 accttgacct cgggcaggcc aaacacggcg tgcggcgccg ccaccgccat gtcgcacatc
    13021 gccatcaggc ccatgccgcc ggccatgcag gcgccattga cacgcgcgat cagcggcacg
    13081 ttgagctggc gcgtgagccg aaacaggttg gccatgccct gcgtgggctc ggagtagtcg
    13141 aacttgaagg actggccggt ctgcaggtcg gcgccggcgc aaaaggcctt gtcgcccacg
    13201 ccggtgagca cgatggcgcg cacctcgcga tcctggttgg cgcggcccag cgcttcgctc
    13261 aggccggcca gcacggcggg gctgatggcg ttgcggcgct cgggccggtt gatggtgagc
    13321 cacagcacgc cgtcgtgctg gcggctgagc agctcgggct cgggggaaga agtcatgggg
    13381 gtatcgctcc gatcgatcag gcagtggaac tggggggctt ggccgcgcgc gccgcgccgc
    13441 tggccagcag ggcgtcgatg tcggccggcg caaagccggc ctcgcgcagc acctcggtgg
    13501 tgtgctggcc cagcgccggc actggccgct ggtggcgagg cacggtgtcg gaccattcgc
    13561 tgggcacggc ggtttccagc acggcgccga ccaccgggtg ttcggtgtgc tggaagaagc
    13621 cggtctgctg caggtgttcg tcgtgcagca gcgattcgaa ggtgtgcatg gggaagatcg
    13681 gaatttccga ctccggcagc agcgcgcgcc attcggccgt gctgcgctgg gccagctcgt
    13741 cggccaccag ctggtacagc gcatcgatgg cctgggtgcg ggtgcggatg ttggccaggc
    13801 gcgggtcggt cttgagcagc tcgcccttgc cgatggcgtt caagaacagc tcccagtgcc
    13861 ggtcggtgta gatcaggcag cagacgtagc cgtcgcgcgt cttgtagggc cggcggttgg
    13921 ggctcatcag gcgcgggtag ctgaagtcgc cccgggccgg ctcgaaggtg cggccgtaca
    13981 ggtggtcgcc cagcacgtag gggatcatgg tttcgaacat cgggatctcg acgctctggc
    14041 cccggccggt gcgctcgcgc gcatacagcg ccgcgctgat gacgccaaag gcatacagcc
    14101 ccaccgagcg gtcggcgatg gtgatcggca ggtagcgcgg cacctcgccg gcggcctcgc
    14161 cgaccacggc ggccagcccg gtggcggcct ggatcatgtc gtcgaacgcc gccttggccg
    14221 cgtacggccc gcgctgcgaa aagcccacca tgctggcgta gatcaggcgc gggttggccg
    14281 ccagcagcac atcgggcccc agccccagac ggcgcatcgc cgccggccgc acgttggtgg
    14341 cgaacacgtc cgcatcgcgc gccagctgca gcagcgcggc caccgcggcc ggctgcttca
    14401 ggtccagcac cacgctgcgc ttgttgcggt tcaggcccag gaacagcggc cccaggccga
    14461 tatcgccgca gggcccgatg ctgcgcgtgg aatcgcccga cggcgcctcg atcttgatca
    14521 cgtcggcgcc cagatccccc agcaactggg tacacgatgg gcccatgaac accgtggtca
    14581 ggtcgagcac gcgcacacca gccagcgggc cggcgggctg ggtggggttt gccgcttgtg
    14641 gcgtcattgc gcctcgatgc ccgccgcgcg gatcacctcg ccccattttt tcgattccgc
    14701 ggcctggaac tgggccaggc cttccggggt gccgagcgcc agcacgccgc tggtgcgcgc
    14761 caggctggcc ttgacttcct cggtggccga ggccttggca aacagctggt tgagcttggc
    14821 gatgacggcc ggcggcgtgc cggccggtgc ataggcggca aaccagtacg acatgtcgta
    14881 gcccttgacg ccggcctcgg ccaccgtggg cacatcaggc aggctggcca gccgcttgcc
    14941 gctgctggcc gccagcgcgc gcagcttgcc gccctgcacc tggggcaggg cggtgggggc
    15001 gtcggcgaac atgaaggcaa agcgcccgcc gatcaggtcg gtgatggcct gcgggttgct
    15061 tttgtaaggc acgttcagca tgtccacgtg ggccatctgc tgcagcagat agccggcgac
    15121 ctggctggac gaggagccgc tgccgaagtc gatcttgccg ggcgccttct tggcggcggc
    15181 cagcagatcg gccaacgact tgtagggcga gtcgggcctg accagcaaca gcatctgccc
    15241 ggtggagagc aggccgatgg gggcgaaatc cttgaccggg tcgtacggca gcttcttgaa
    15301 caggtgttcg ttggccgcgt gcgtggtgtt ggtggtcatc atcagcgtgt agccgtcggg
    15361 cgccgccttg gccacgtgct gcgcggcgat gaaaccgctg gcgccgcctt tgttgtcgac
    15421 caccacggac accttggctt cgtcgcccag cgccttggcg tacaggcgtg ccatctggtc
    15481 ggtgccgctg ccggggccaa acggcaccac cagcgtgatc ggcttgctgg gaaagtcctg
    15541 ggctgcggcc ggttgtgcca gcagcgtggt ggccgccgcg gtcagcgcga gcagggtgtg
    15601 gatgggtcga aagctcatgg tgtctcctgt aggttctggg tgaaaggcgt gacggtcgcc
    15661 ggcggcaggc cgtgcgctgc gttgtccgcg cccaggcgcg gcgcggccga gcgtggcagg
    15721 gggcgcgcgc cgtcgatgga aaacggcagc gcggtgagcg cgaaatcctc gcccggcagc
    15781 ggcgccagga tgcccagcgc ctgcacctgc ggatgcgcca gcgcttcggg tacgctgtgg
    15841 atgggcgcgc agggcacgcc cgcggcctcg aagcgcgcgg cccattcggc gcggggtcgc
    15901 gcgcgcagca gcggctgcag ctgcgccagc agctcggcct tgtgcgccag ccgcgcgcga
    15961 ttgctggcaa aacgctcgtc ggccagccag tcgggccggc ccagctcggt cgccagcttg
    16021 gcaaacaggc ggtcgttgcc gcagcagatc aaaagcggtg cgtcctgggt gtcgaacgcc
    16081 tcgtagggca cgaagccggg gtggcccgaa cggtcgcgca gcggcaggcg accttcgttg
    16141 acgtaggcat cggccttctg gccgttccag accagggcgg tttcgagcag cgaggtctgc
    16201 aagatgccgc cgcgcccggt ctgctggcgc cgctgcagca aggccagcgc gccgatcacg
    16261 gcccacatgc ccgagccctg gtcgcacatc gacgcgcccg agcgcatggg cgggtcatcc
    16321 gggccgccgt tgatgctgga caggccgctg aaggcctgca cgaggggttc gtagcccggc
    16381 tgcatggcca tcgggccggt gctgccgaag gccgagatct cgcagtagat caggcgcggg
    16441 tggcgtgcgc acaggctggc cccgtcaatg cccagcttgg ccggcacgcc ggggcgcagg
    16501 ttgtggatga agatgtcggc ctcgcccacc aggcgctcga acgcggccag cccgtcggcg
    16561 cttttcaggt ccagcgccac cgactgtttg ccgcgattga agatgtggaa gttgagcgcg
    16621 tcgccatgcc ggaacgccgg tcccatggcg cgcgcgtcgt cgccgccttc gacgcgctcg
    16681 accttgacca cttcggcgcc caggtcggcg aagatcgcgc cggcaaacgg cccggccagc
    16741 acctggccca gctccagcac gcgcacaccc gccagcacgc ccatcgcggt cgcattactc
    16801 acgcttgtct cctttggatg aaaggcagtc taggctcgca cgtctgatat gggaatagct
    16861 atatttctat atctgtgata acgattgggg atcaatgcca tgcgccttga cctgagcctc
    16921 cggcagctcg aagccttcgt acaggtggcc gacagcggca gctttcggcg cgcggcggcg
    16981 cggctggggc aggcgcaacc ggtgctcagc cgcctgattg gccaggccga gcagacgctg
    17041 ggcgcgcgcc tgttcgaccg cgacacgcgc cgggtcgaga tcacgccggc cgggcgcgag
    17101 ctgctgccgc tggcccagcg cctgctgcgc gacttcgacg acacgctggg cgactttggc
    17161 gccttcatgg ccgggcgcag cggccgcgtg tcgatggtgg tgctgccctc ggtcagtgtg
    17221 gcctgggtgc cgcgcgccat ggccgcgttt gctcgcacgc atccgcaggt gaactttgcc
    17281 ctcaccgagg cgccggccga caccctgctg gcgctgctgg aggccgggcg cgccgatttc
    17341 ggcatcagcg tgcggccggc gccgcagcag cgcctgcact accgtcacct gcaggacgac
    17401 ccgttcgtgc tggtgtgccg gcgcgacgat ccactggcgg cgcgcgcggc cgtgccctgg
    17461 tcggtgttcg gcatgcgcga ttgcatcatg agcgcggcgc acagcagcat ccgccaggtg
    17521 acggacgcgg tgtttctgcg ccagcgcacg ccgcccagcc cggtgctgga ataccccagc
    17581 gtggcggcgg ccggcgcgct ggtcgcggcc ggcctgggca tcacggcgct gccggtgctg
    17641 gcgcttcagc tggtggacat gcgcgagctg gtggcggtgc cgctggtgcg cccggccatg
    17701 gcgcgcccga tcggcatcgt cacgcgcatc gggcgttcgc tgtcgcctgc cactcaggcg
    17761 ttcatggcgt cgctgcaggc ggcgccggcg ggggcgcccg acgcgccgtg aggtcaaagc
    17821 gcagcagccg gcactcgatc gggccgttcc acagcggcac gcggcgcgat tccttcaggc
    17881 gcaggcggcc gggcagcttg ctgtcgggcg tcagcagcca ggcgctccag ccggcgtagt
    17941 ggctcttcca gtgcgcggcc aggcgggcga agaaatcgtc gccggcgttg acatcgtcgg
    18001 caggcgccgc gccccccgcg cgctggcgcg ccagcgtgcc ggactgaaag gcctcgcgcc
    18061 cgccgcgcgc gccggccacg ccggcggcgg cgatgcgctc gccatagggc ggattgagca
    18121 gcagcacgcc cggctccgcg cagggcggca ggcgctgcag tgcgtcgccg ccgcgaagct
    18181 gcacggcggc cgccacgccg gcgcgccggg cgttgcgctg ggcgaagtcg accatgcggt
    18241 gggcgacgtc gctgccgaag atgcgcaccg ccggggtttg cgcatcattt tggtctccgg
    18301 cgcttgtgga gcaagcgcca gcagcttcgg ttttgatagc gttccagcct gccggatcgt
    18361 gcggccgcag ctgctcgaag gcaaagcgcc gctggccgcc ggcgggcagg ccgagcagca
    18421 gttgcgccgc ctcgatgacg atggtgcccg agccgcagca ggggtcgtac agcggcagcg
    18481 gcgacacgct gcgcgtggcc ggctgccacc agccgcagac cgccagcatg gcggcggcca
    18541 gggtttcctt cagcggcgca tcgcccttgt cctggcgcca gccgcgcttg aacagcggct
    18601 cgccgctggt gtcgatgtag agctgggcgt gcgtggccgt caggtgggcc tgcacccgca
    18661 cgtcggggcg ctgggtgtcg atgctggggc gcaggccgcc ggcctgcgcg cgaaaacggt
    18721 cggccaccgc gtccttgatg cgcagggtgg cgaagttcag gcttttcagc gggctggcct
    18781 gcgccgtcag atcgacgcga aagcgctggc gcggggtgaa ccactgttcc cacggcacgc
    18841 cggcggccag cgcgtacagg tcctgctcgc tctggtaggg gccgtgggcc agctcgatca
    18901 gcacgcgctg ggccaggcga ctgtggaggt tcaggcgctg catcagcgcc cagtcgccgc
    18961 gcacgcgcac gccgccgcgc aggctcaaga ggtcttcgcc caccaggcct gagaggccat
    19021 gcacctcgtc ggccagaaaa tcctccacgc cgccggcgca gggcagaaac agggtcagcg
    19081 cgttcatggc gtcgctgccg cggcgcaggc cagcagcttg ccctgaatcg gcgccgggat
    19141 gggggtgacg cggcgcgaga cgggatcgat gaacacgatg ccggtcttgc cgcgcgccac
    19201 ctcgcggccc tggctgactt cggtcatgcg aaacaccaga tcgaagccgt agcggttgaa
    19261 gtcggccggc tgcatctgca cgcgcagcgt ttcgccgtgg aaggcctcgg acttgtactg
    19321 cgccagcatg tcgcccacca cgatggcggc gccgtccaca tggccttcgc ggtagcccag
    19381 ggcgtggaaa aagcgcagcc gcgcctcgga caccagcgcc agcagctggg cgttgtccag
    19441 gtgcccgccc tggttgacgt ggctgatgta gatctgcagc tcggtggcaa agccgaactg
    19501 cgcgggaagg tcgaagacga tgcgggccat ggtgaaacgg gggctcagtg gctcttgcgc
    19561 aaagaggcgg tgggcaggcg cagggcctcg cggtacttgg ccacggtgcg gcgcgcgcag
    19621 tcgatgccct gggccttcaa ctgctcggcg atctgggcgt ccgacagcgg gcgcgcgcgg
    19681 tcttcggctt cgatcagctg ccggatcagt gcgcgcaccg cggtgctgct ggcgtggctg
    19741 cccgattcgg tgcccagcgc cgagccaaag aagtacttca gctcgaaggt gccgcgcggt
    19801 gtggccatgt acttggcggt ggtgacgcgc gagatggtcg attcgtgcac ccccagctcg
    19861 tcggccagct cgcgctggat cagcgggcgc atggccagct cgccgtggat gaagaagtcg
    19921 cgctggcgca gcacgatggc gttggccacg cgcaggatgg tgtcgaagcg ttgctggatg
    19981 ttgcggatga accagcgcgc ttcctgcagg cgttcgctga gcgccgggtg cttgtcgctg
    20041 ccacgcaggg cgctggcgta ctgctcgtgc acgcgcagcc gcggcaccac gtcggggttg
    20101 agcgccacgc gaaagcgcgg ccgctcggcc gtgccgacgg cgctgacgat gacgtcgggc
    20161 acgacgatct ggcgctcgac gtcggcaaag cggcgccccg gcttgggatc gagccgcccg
    20221 atcagggcca tggcggcgcg caccgcgtcc tcgctctcgc cgacgagctg cgccagcttg
    20281 cggctgtcgc gccgcgccag cagctcgacg gcgtcgtggc gggcgcagat gcgcagcgcg
    20341 gtggcgatgc gggcctgctc ctgcggcgcc agcgcgcgtt cggcctggcg gctgcgcaac
    20401 tgcagcgtca gacattcggc caggtggcgc gcgcccacgc cggccggctc cagcgactgc
    20461 agcagcttga gcgccagcgt caggcggtgc accagctcct gcatctgctc cagttcgtcg
    20521 ccgtcctcgg tcaggctggc ggccagctgc gccagcgagt cttccaggta gccgtcgtcg
    20581 ttcagcgagc cgatcagaaa gcgcagcgcc gccgcgtcct cgggcgccag gcgcagcgtc
    20641 agcgcctggc gcagcaggtg ctcggccagc gattcctggc cgccgacgcg ctcgcccggg
    20701 tcggtgtcgt cgtcgcccgc ctcgcgctgg cgcgccggtg cctcgccgcc ccattcggcg
    20761 tcgtccgggg cgatgtccac gctgccgtcg ccgtcccagt cgtccggggt caggggttcg
    20821 gcatcaaaat cggcttcggc gcttgtggaa tctgcgccag cagctcctga atcaatagcg
    20881 ttttcgtcgg tgcgttcgcc ccggtcggcc ggcgtcggcg cggctgcgtc gccggcctcg
    20941 tcgtcgcgct ccagaaacgg gttgtcggcc agcatctgct cgacttcgcc gctcagctcc
    21001 agcgtcgaca gttgcagcaa ccggatcgac tgctgcagct ggggcgtcag cgccagctgc
    21061 tgcgccacgc gcagcgaaag cccgggtttc acagccggaa atgctcgccc agatagacgc
    21121 gccgcacctc ggggttggcc acgatgtcgg ccggcgtgcc ggtggccagc acgcgacctt
    21181 cgctgatgat gacggcgtgg tcgcagatgc ccagggtttc gcgcacgttg tggtcggtga
    21241 tcagcacacc gatgccctgg ctcttgagga aggcgatgat gcgctggatc tcgatcaccg
    21301 cgatcgggtc gatgccggcg aaaggctcgt ccagcaggat gaagcgcggc tgcgtcgcca
    21361 gcgcgcgcgc gatctccacc cgccggcgtt cgccgcccga cagcgccggc gccggcgaat
    21421 cgcgcaacgg ctcgacatgc agctgctgca gcagctcgtt caggcgctgc tcgatggcgt
    21481 cgcgccccag gggccgaccc tgggcgtcca cctgcagctc cagcaccgcg cgcacgtttt
    21541 cggccaccgt cagcttgcgg aagatcgagg cctcctgcgg caggtaggac aggcccaggc
    21601 gcgagcgccg gtggatcggc atgtgctcga tcggctggtc gtcgatggcg atgctgccgg
    21661 cgtcggcacg caccaggccg acgatcatgt agaaggaggt ggtcttgccg gcgccgttgg
    21721 ggcccagcag gccgaccacc tcgccgttga ccacgtccag cgacacgtcg tgcaccaccc
    21781 ggcgtgcgcc gtagttcttg cgcaggccgc gcacctgcag ccggctctgg ccgtgcgcgc
    21841 gcggcgcgtc ggcggcggcg ggggcgtcaa agcgcgacgc cagcgcctcc agcgccggtt
    21901 cgtgcacggg cgtgttcact gtccgcttcc ttcgggcagc gcagcggccg ggcgcagctg
    21961 cagcgccggt gccgaagccg cggacgcggc gctggcggcc tggcgcggcg ccagcgtggc
    22021 gcgcacgcgc ccgcccggcg ccgccgaggg tgcgccggcc gcgggtgcgc cgtccaccgt
    22081 gtacacctcg gtgcggttgt tgtagacgat caggctgccc agcacctggt ccagctgggt
    22141 ggcgccctcc aggcgccgca tctcggcgcg gcggatcagg cgcacggtgt cggtcttgcc
    22201 gtcgtattcg atggtttcgc cttcgccttc gatgaactcg ttcacgccct cgcgcttctg
    22261 acggaagaag gcgcgcttgc ccggctcgcc cgacagggtg ccgaactggt tgccggcgct
    22321 gtcctggcgc acttccaggc gggcgccgcg catcacgatg gtgcccttgg tcaccagcac
    22381 cttgccggtg aaggtggact gctgcttctg ctcctcgtag cgcagcgcgt cggcctcgat
    22441 gttcatgggc ttgtcgcggt cggcgcgctc ggcccaggcc ggcgcggcca ggccgagcag
    22501 cagggtggcg cacagcaggc gcaggctggg agcgaacggc ggtacggacg acttcatcgc
    22561 gaaggcacgg tttttgcttt cagtatgaaa cattctaagc ggccgccatg cgtgccgcag
    22621 cggtgcggca catcaaaaaa gcccgccaag gcgggctgct gcgtgcgtcg cccgagcggc
    22681 gcgtcacttc ttgcgttcct gggcgatcag gttgttggcg atctggatca tgcgctcgaa
    22741 gccgccggcc atcgagccgt cggtgtagta gcgcccggca atgcccagcg ccggcgtgcc
    22801 ctcgacgtgg taggcctcct gcaactgcac cgcgcggcgc agcttgttgg cgatgccgaa
    22861 cgatttgtag gcggcctcga acttggcccg gtccacgccc tgctcggcga tccagggcag
    22921 cagcacgtcc ggcttgtcca ggcgcttgcg ctcggtctgc agggcctgga acaccttcat
    22981 gtgcaccggc tgctcggtca ggcccagggc gtcgagcgcg taatagatgc gctgcagcgg
    23041 ctcgaagctg ctctggaagc cgacatgttc gcgccgcacc gtcacgtcct tgggggcggc
    23101 cttcttccag gcctcgaaca ccggctcgaa gttcttgcag tgcgagcagc cgtaggagaa
    23161 gaattcgatc acctcgatct gcccggccgg cgcgtcgacc ggggcgcggt ccttgagcgt
    23221 ggcgtagccg gggcccgact gggcgcgcgc cggcagtgcc agcaggccgg gcagggccag
    23281 cgcgccagcg agggtgaatt cacgacgatt catggaaacg gactccttgt ctatggactg
    23341 ggttcagagc gtcgccgtcg ggcggagttc ccgcgccgcg gtcatttctg cacccgcacc
    23401 agcgcggcgt cgaagccctc gccgtccagc cgctgcttga cgcggtcggc cgcatcgcgg
    23461 ttctggaacg ggcccacgcg cacccggtag acggtgcgac cggcctgttc gcgctcgctc
    23521 acgcgcgctt ccacgcccat cagcgacagg cgggcgcgct gggcatcggc gtcttccggg
    23581 gtgcggaagg cgcccgcctg cacgaagtag gcgaaggggt cggcggcggc cgccgcggca
    23641 ggcgccgccg gcgcagcgcc gctgcgcgcc gcggccagat cgcccagcgg atcgctggac
    23701 gggcgggccg gcgccggccg cgcggtcacc gcggggggtg ccggctcggc cgtcttgtcg
    23761 gcgccgcgcg ccggggcgcg cgcgtcggcc ggcttgctgt cgggggccgg gcgggccact
    23821 tcggtcggtg ccgggatggg cgccggcgcc ggtgccggca ccacgctgcc gctggcggcg
    23881 gggcccgggc cggccgggcg ggtgccgttt ttgccacgca gcgcggcgtt cgggtcccag
    23941 tccttgttct tttcggcctc ggccgcgtcc tggccgttgg tgcgggtggc gttcttgctg
    24001 accagcggca ctggcacctt ggccacgtag atggccaccg ccagcgccgc cgccaggccc
    24061 accagcaccc cgagcagcag acccaggaag gtgccgccgc gttgcttgca gatcatcatc
    24121 gttggagcca tgccatcaca tccgggtggg cgccgtgacg cccagcaccg tcaggccatt
    24181 gtgcaacacc tgggcggtgg cggcgaccag ggccaggcgc gcggtcttca ccgcggggtc
    24241 gtccaccagg atgcgctcgg cgtcgtagta gctgtggtag gcggcggcca actcgcgcag
    24301 gtaaaaagtg acgtcgtgcg gcgcgaagtc ctgcgcggcg gcgctcagca tgtcggggta
    24361 gcgggccagc tgcagcatca gcgcctgcgc cgccggcgtg tccagcggcg tcaggtcggc
    24421 gtcggccagc gtggccgcgt cgccgcccca ggcctgcagc accgagcaga tgcgcgcgtg
    24481 ggcgtactgc acgtagtaga ccgggttgtc gttgttctgc tgtacggcca gatcgacgtc
    24541 gaaggtgtat tcggtgtcgg gcttgcgcga cagcaggaaa aagcgcaccg cgtcggcgct
    24601 ggtccagtcg atcaggtcgc gcagcgtgac gtagctgccg gcgcgcttgc tgatcttgac
    24661 ctcctggccg ccgcgcacca cgcgcaccat ggtgtgcagc acgtagtcgg ggtagccggc
    24721 cggaatgccg acgccggcgg cctgcaggcc ggcgcgcacg cgcgcgatgg tgccgtggtg
    24781 gtcggtgccc tggatgttga tggctttcct gaagccgcgt tcccacttgg tgatgtggta
    24841 ggccacgtcg ggcacgaaat aggtgtagcc gccatcctgc ttgcgcatca cgcggtcctt
    24901 gtcgtcaccg tagtcggtgc tgcgcagcca cagcgcgccg ccttcctcgt aggtcttgcc
    24961 ggcgtcgatc aggcgctgca cggtggcttc gacgcgtcct tcggcgtaca ggctggactc
    25021 caggtagtag cggtcgaact tgaggcgaaa ggcctgcagg tccttgtcct gctcgtggcg
    25081 caggtaggcg acggcgaact ggcgcatgcc gtccaggtcg tccacgctgc cgctggcggt
    25141 gaactggcgg tcgtcggcct gcaccgtgcg gcgggccaga aaatcgtccg cgatgtcctg
    25201 gatgtagtcg ccgttgtagg ccgcctcggg ccagtcggcg tcgcccggtt tcaggccgcg
    25261 ggcgcggcat tgcaccgagg tcgccagggt gtcgatctgc accccggcgt cgttgtaata
    25321 gaactcgcgc tgcacctgcc agccctgact ggtgaacagg tggcagatgg cgtcgcccag
    25381 cgccgcctgg cggccgtggc cgacgtgcag cgggccggtg gggttggcgc tgacgaactc
    25441 gaccagcatc ttctcgccgc gcgccgggcg gctgccgtag cgcgcgcctt ggctcagcac
    25501 ctcgagcacc acctgctgct tggcggcggg ctgcaggcgg atgttcagaa agccggggcc
    25561 ggccagctcg atcgccgaca cccagcgctg aaaggccggc gcggcctgca acgcttgctg
    25621 aagctgctcg cccagcgcgc gcgggttggc cttgagcggc ttggccagct gcatggcggc
    25681 ggtgcaggcg aagtcgccat gcgcggcctg acggggcgat tcaaaggcgg ctttcgaccc
    25741 gctgccgggc cacagccgct ccagctcggc cgccagcgcg gcgagcagtt cggtcttcac
    25801 ggattgcatc ggcggatttt acggggcggg cgccgggccc cggggtgtgc cgcccaggcc
    25861 cctgggtgat gcatcgaatc gggctctggc gctcatccag caagcgccac aagctatgat
    25921 aaaaatagca aatgccggct tcacgcgccg taaccacggg ccaggaacac cgccgccagc
    25981 ggaatcaccg ccaccaggtg ggtctcgatc atcaccaggc ggcgcacgcc ctgcacctcg
    26041 tgcgctgccg gcaggctggc gtcggccgcc agcgcccgct gccagcgtgc gatgcgccgc
    26101 gtcggcccca gcatcagcac ggcggcgacc aggaacagcg tcaacttcag gtgcagcagc
    26161 gggttgctcc agtaccaggc ggcgcccttg gcgccccagc agatgcgagc cagcccggac
    26221 agcagcacgg cggcggtggc gatccacaga atgcggtcca gccgcgccag ccgctggacc
    26281 acggtggcgt tgagccattc ggcgcggcag atcgccgcct gcgcggacgc gaacaccacc
    26341 cagccgatca cggcgctgat gtgaaagaag gcaagccagg cttcgagcat ggcccggatc
    26401 gtacccgggc cgcccaggcg tcagcccggc cggcgccaga aggccggatc gccgtagcgc
    26461 tggcgcaggt gatcgaccca cagccgcacg cgcagcggca ggtggcgcgt ggggtgcggc
    26521 acgatggcgt agatgccgtt gggcggcgcc gcgaacgcct ccagcaccgg caccagccgg
    26581 ccgctggcga tggcgtgctc gacttcccag gtcgagcgcc aggcgatgcc gtggccggcc
    26641 aggcaccagt cgtgcagcac ctggccgtcg gtgcagtcca gccggccttc ggggcgcagg
    26701 tggatgacgt cgtactcgac gtcgctgcgc tggatcggca cgcccgcccc cgcacggggg
    26761 cgttcgtcat tctgcccgtg ggcctggcgc ggcacgcgaa aggcccagcc gcgcgactgc
    26821 gaggcgtcgg acgacagcat cagacagtcg tgctgggcca gctcgcgcgg gtggcgtggc
    26881 cggccgcgcc gcgccaggta ggcgggcgcg gccacgcaca ggcgctggtt gtcggccagg
    26941 cggatgctga tgagcgacga gtccggcaga tcgcccacgc gcaccgcgca gtcgtagcct
    27001 tcggccgcca gatccaccac ccggtccgac aggttgagcg aaatgcgcag gcgcgggtgc
    27061 agggcgtgga actcgggcac cagcggcgcc acatggcggc gcccgaagcc ggccggtgcc
    27121 gtcacgcgca ggtgaccgct ggcctgcacg ccgccggccg acacgctggc ctcggccccc
    27181 gccacctcgg ccaggatgcg ctggcagtcg tccagaaagg ccgtgccttc atgcgtcagc
    27241 gcgatgcggc gcgtggtgcg caccagcagc ttgacgccca ggtgctcctc cagcgcgtcc
    27301 aggcgccggc tgaccaccgc cggggccacg ccctcggcgc gcgcggcggc gctcaggctg
    27361 ccgcgcgtgg ccaccgaggc gaaggtctcg aaggccttga gcttgtccat gcgcggcaat
    27421 tatgcgcagc cgcgcccatc ggttgcggcc ggcgcgccac aggccgctgc gccatgcggg
    27481 cgtgacgtgc gatcatgcgc gctcacatct gcctgcaccc atgcgagccg aatccgcccc
    27541 gccgatcgac aggcgccaca tcgatgtgcg cacgatgagc ctggcgcagc tgcagaagct
    27601 ggccgagcgc ggcagccggc gcgcccgcgc cgaactggag gcgcgcatgc gggcgcccgg
    27661 ccccgtcctg ctgccggcgt ccgtgccggc cgaccgcacg gcaccatccg cgccgagccg
    27721 gcacccgccg acgctgaccg cgcgcgccga tccgccgccg caggccaccg ccgccggagc
    27781 ggcagcgccc gcgcccggcg cggtgccggc tgggcagggg gtgccggtgt cggccgacgc
    27841 gctggcgcag caactgcacc tgatcgcccg gcaggacgag gcgcacgccc gcgccgccgg
    27901 gccgccgcgc ctggtcggcc tggtgctgac ggcctggggc gtgctgctgc tgctgggagg
    27961 gctgctgatg ctcgggcgcg gtggcgcggc gctgtactac ctgttctgcg gcctgggcgc
    28021 ggccgccgtc ggcgcgctgc tgatgcggcg cagccgctgg gccctggccc tgcatggcgc
    28081 cctgctgccg ctggcgctgg gctgggcctg ggccggtgcg gggcgccaca gcctgcccct
    28141 gagcgtggtg caggcggggc ccttgctgat cccggcgctg tggatggcgc tgcgccaggt
    28201 gcgcgaggcg ctggaatgat atgcattgaa cctgggttaa aagtgcctcc ggcgcttgat
    28261 gggcaagcgt cagcagctat cgtattcata gtgaactgag ggttccgatc cgacccgggg
    28321 ggcgccgtgc gggcgttggc tggccgatct ttgcttcaga cgcaaaggtt ttttggtttt
    28381 tcacctgttt gatgcccaaa acaaatcgaa taaagtgccc acaccgggcg ccaggcgcct
    28441 gttttttctt tgtttctaca aggccacccc atgaccgcac gcacccgcat tcacgacctg
    28501 cacgttgccg acacgctgta cgagttcatc aaccgtgagg ttctgcccga cgtcggcgtg
    28561 gacgcggcgc gcttctggca gggtttttcg gccatcgtga ccgagctggc acccaagaac
    28621 atcgccctgc tggccgagcg cgaccgcctg cagaaggagc tcgacacctg gcaccaggcc
    28681 aaccccggcc cgatcaagga catgcgcgcc taccgcgcct ttctggagcg catcggctac
    28741 ctggtgccgc agccggccaa ggtgaaggtg accaccaagc gggtcgatgc cgagctggcc
    28801 gtgcaggccg gcccccagct ggtggtgccg atcctcaacg cccgctatgc gctcaacgcc
    28861 gccaacgcgc gctggggcag cctgtacgac gcgctgtacg gcaccgacgt gattgccgaa
    28921 accaggggcc tggagaaggg cgccaagtac aacccgcggc gcggtgccaa ggtgatcgag
    28981 tgggcgcgcc acgtgctcga ccgcacggcg ccgctgaaga agggctcgca cgtcgattcg
    29041 gtcgcctacc tggtgcgcga cggcgagctg gtggtcaagc tcaaggacgg caagagcagc
    29101 aagctggccg acccggcgca gttcatcggc taccagggcg agcccaaggc gccgtcgtcc
    29161 atcctgctgc tgcaccacgg cctgcacctg gacatccgca tcgacccggc gtcgcccatc
    29221 ggccgcaacg acccggccgg cgtggccgac ctggtggtgg aggcggcggt ctccaccatc
    29281 ctggatctgg aggactccat cgccgccgtc gatgccgagg acaaggtgct gggctaccgc
    29341 aactggctgg gcatcctgcg cggcacgctg accgagtcgt tcgacaaggg cggcaagacc
    29401 ctgacgcgcg gcctcaaccc cgaccgcctc tacaccgccg ccagcggcaa gggccaggtg
    29461 cggctgcacg ggcgctcgct gatgttcgtg cgcaacgtcg gccacctgat gaccaacccg
    29521 gccatcctgt atcgggcggc cgacggcagc cagcgcgaga tccccgaagg catcatggac
    29581 gccgtggtca ccaccaccat cgcgctgcac gacctgcagc gcaagggcgc gaacggcatc
    29641 cgcaactcgc gcaccggcag cgtctacatc gtcaagccca agatgcatgg cccggccgag
    29701 gtggcgttcg ccgccgagct gtttggccgc gtcgagcaac tgctgggcct tgcggacagc
    29761 accgtcaagc tgggcatcat ggacgaggag cgccgcacca gcgtcaacct caaggcctgc
    29821 attgccgccg ccaagagccg cgtggccttc atcaacacgg gctttctgga ccgcaccggc
    29881 gacgagatgc acagctgcat gcgcgccggc ccggtctacc gcaagagcga catgaagacc
    29941 agcgcctgga tccaggcgta tgagcgcaac aacgtgctgg tcggtctggc ctgcggcctg
    30001 cgtggcaagg cgcagatcgg caagggcatg tgggccatgc ccgacctcat gaaggcgatg
    30061 ctggagcaga agatcgccca ccccagggcc ggcgccaaca ccgcctgggt gcccagcccc
    30121 accggcgcca ccctgcacgc catgcactac caccaggtgc tggtgagcga ggtgcagcag
    30181 cgcctggagg ccgagctggc ccaggccgac ccggcggcgc tgcgcgacga gctgctgacc
    30241 ggcctgctga ccatcccggt ggtgagcgaa gacaagtgga ccgccgccga gaagcagcag
    30301 gagctggaca acaacgccca gggcattctg ggctacgtgg tgcgctgggt cgatcagggg
    30361 gtgggctgct ccaaggtgcc cgacatccac aacgtcggcc tgatggaaga ccgcgccacg
    30421 ctgcgcatca gcagccagca catggccaac tggctgctgc acggcgtggt cagcgaaggt
    30481 caggtgcgcg ccacgctgca gcgcatggcg gcgctggtgg accagcagaa cggcggcgac
    30541 ccgctgtacc gccgcatgca cgaccacttc gagcagtccg ccgccttcca ggccgcctgc
    30601 gacctggtgt tcaagggggt gcagcaaccc agcggctaca ccgagccact gctgcacgcc
    30661 tggcgccaga aggtcaaggc cggcgcttga aaaagcatag cttgcggcgc ccgaaaggcg
    30721 ggcgccgcaa gctgttcaga ccatgacaaa gggcgccgcg gcgcccttgg ttctgtgccg
    30781 tcgcggtcag caggtggttg cgcccgctca ggcgcgggcc aggccctgct tgtgctccca
    30841 ggccgacttg caggccacgc agtgctgcgt catgggttcg atctgcaggc gctcgaaggg
    30901 aatctggcag ccgcattcgt cgcagcggcc gtaatcgccg gcgtccatgg cttccagcgc
    30961 gcggtcgatg cgcgccagct cttcggcgtc cagcgcggtc aggcgcgtgt ccacctcgcg
    31021 cgtctggttg gccaggcggg cgttttcgtc ctgcgacacg ctgccggcgg tgttttcggc
    31081 cggcgccagg ttggcgcggt tctgctgcat ctgcgcgtac agctcctggc ggcgggccag
    31141 cagccgctgt ttcagctgct cgagttgggc gggggtcagg gtttggctca tgctccatct
    31201 ttcctgcaaa gtcgcggatc accatgccgc gcggcggcgc ggcgcttcat cccgcagaat
    31261 accacccaca tgccgctgtc cgcgccaccg atcgacccca cgcgctgccc cctgtgcggg
    31321 cagccgaacc ggtgcgcgca gcaggcgcgt cctgccggtg cgccggcggc gccgcactgc
    31381 tggtgcatgg agcggcgcgt ggccccggac gcgctggcgc gcgtgccggc gcaggcgcgc
    31441 gggcgcgcct gtctgtgccc gtcctgcgcc accccgaggg cgtgaacgcg cagccgccga
    31501 tgcacgatcc gcccccgtcc agttcggccg aggcgctgcg cgcgcgccat ggcgcgcgct
    31561 ggcgctggct gctgctgctg acggtgatgg tgggcaccat ggcctcgatc atgtcctcca
    31621 ccgtggtcaa cgtggcgatt cccgggctgt cgcagcagtt caccctgggg caggcgcgcg
    31681 cacagtgggt cagcagcggc ttcatggtgg cgatgacggt gtcgatgctg accacgccct
    31741 ggctgctggc gcgcttcggc taccgcgcca cctacaacgg ctgcatgctg ctgctgctgg
    31801 ccggcggcgt gctgggcggc gtggcgcaga gctacccgct ggtgctggcc gcgcgcgtgg
    31861 ccgaagggct ggcggcgggg gtggtgcagc cgattccggc ggtgatcatc atgcgcgcct
    31921 tcgagcccaa cgagcagggc cgcgccagcg gcctgttcgg catgggggtg gtgctggcgc
    31981 cggcgctggg gccgtcgatc ggcggcgtgc tggtggactg gttcggctgg cgctcgatct
    32041 tcttcatggt ggcgccgttc tgcctgctgg cgctgtggct ggcgcgccgc tacgtgccga
    32101 ccacggcacc cggcggcgcc gccacccggc gcgacgagac gctcgacggc gtcggcctgc
    32161 tgctgggcag cgccggcacg ctgtgcctgc tcaacgggtt ggtggcactg cgcgccgggc
    32221 cggcggtcga agcggcggcg ctgctggcgc tggcggcgat ctgcctggcc ggcttcatcg
    32281 gctggcagcg ccgctgcgtg cgcctcgggc gcgcgccgct gatgaacctg gcgctgttcg
    32341 cctggcgacc gtttgccatg ggggtgctgg tggcgctgat ctacggcacg gcgctgttcg
    32401 gctccaccta cctgctgccg gtctacatgc agatggggct ggggttgtcg gcctccagcg
    32461 tgggcgccat cctgctgccg gccggcctgg tgctggcggc caccatcccg ctggtggcgc
    32521 gctgggccga ccggctgccg gccagcggcc tggtgaacac cgggctggcg ctgctggcac
    32581 tgtcgttcgc ggccatggtg acggtggggc tggcgcccgg gctgtggcac ctgacggcgc
    32641 tgggcatcgt cgggcgcatc gggctgggct tcatcctgcc ctcgctcaac ctgggcgcca
    32701 tgcgcgggct ggcgctgccg ctgatcccgc agggctccag cgtcatcaat ttcgcgcgca
    32761 ccctgggcgg ggcggccggc atcagcctgt gcggcatcgc actggaatgg cggctggcgg
    32821 cgcatggcac ctcgctgacc gaaggtgcgc ccagcgcggc gcggttggcg gcgttcgacg
    32881 aagtgttcct gatgctggcc gcgctgtgcg gcatggccat gctggcggcg cgccacctga
    32941 gcacgccggc cgcgggtgcc gaccgataaa ctgcccgcca tgtgccagct gctgggaatg
    33001 aactgcaaca cgccgaccga cgtgagcttc agcttttccg gcttcgcgca gcgcggcggg
    33061 cgcaccgacc accatgccga cggctggggc atcgcctttt tcgaagggcg tggcgcgcgc
    33121 gtcttcctcg accccggggc cgccgccgat tcgccggtgg cggcgctgat ccgcagctac
    33181 ccgatcaaga gccgcaacgt gatctcgcac atccgcaagg ccacggtggg cgcggtgacg
    33241 ctggagaact gccacccctt cgtgcgcgag ctgtggggcc gctactgggt gttcgcccac
    33301 aacggcgacc tgaaggacta cgcgccgcgc ctgcacggcc actttcatcc ggtgggcagc
    33361 accgacagcg aacacgcctt ctgctggctg ctgcaggagc tggccaaggc gcacgccgac
    33421 gtgccgccgg tggccgagct gacccgcacc ctgcgcgagc tgctgcccca ggtggcgcgc
    33481 cacggcagct tcaacctgct gctgtccaac ggcgaggcgc tgtgggcgca cgccagcacg
    33541 cacctgtcct acatcgaacg gcgccacccg tttgccagcg cccggctggc cgacgaggat
    33601 ctgagcatca acttcgccga ctgcaccaca ccgagcgacc gcgtggccgt gatcgccacc
    33661 cagccgctga cctgcgacga gcgctggacg gccttcgagc cgggcagcct gaaggtgttc
    33721 gtggacgggg cattgcagaa tctgtgagcc cggttgggct ccagaaagct attaaaacga
    33781 tagctgctgg cgcaggtcgt tgctgcgcca gagctgaatt tctatgttga tgggcgctcg
    33841 ccgcgcaccg tgctttcgcg cactgcgcgc cggacgatgg gcggctgccc gccgatgtga
    33901 aaggccagct tgcgctcgcg cagcgcggcg tcctgcacgc tgacgcgctc gaaacggcgc
    33961 aggtgctcgg tccaggaggc gtcctcgatc acctcgatga agcggcccgg gtcgttgata
    34021 tccgacagca gctcccagcc caccgcgccc acgcgcaggc gcgagcggcg gctttcttcc
    34081 atcagggcgc ggaaggccgg cgcctgggcc gggtcgatct ggtattcgac ggtgacgatc
    34141 acgtggccat ggggcggcgg cgcctcggcc tgcggcaggg gccaggggcc ggccggcgtg
    34201 gggtcgtcga tgctgccgcg gtcgggccag cgccaggtcg ccagcgccat gctggcgatg
    34261 ccgctggcgg caccgatgcc cagcgtgacg ggcacgctgc tccaggtcgc cacctggccc
    34321 cacacggcgg cgccgacggc ggtggcgccc atgatggcca tctggtacat cgacatgccg
    34381 cgcgcgcgca cccagtcggg caggctgatc tgcgccgaca cgctgagcga gttggcggtg
    34441 gtcagccagg ccacgccgca cagcagcatg gccggcaccg ccagccacag gctgtgggtg
    34501 aacgccatca gcgccatcat cagcgcctgc gtgcaggcgc cggccagcac cagccggtcg
    34561 cgcgtgaaca tctggcgcca ccgcggcagc acgaaggtcg agccgatggc gccggcgccc
    34621 atggcggcca gcagcacggt gaaggtgccg gcgtggccgc cctcgatctg gcgcgccacc
    34681 agcggcagca gcgcgatcag cgcggtggag tgaaagaaga acagcgccac gcgcagcagc
    34741 acccccttga ggtggtagga ctgcatcaca tactgcaggc ccacgcgcat ggcgccgcgc
    34801 aggctctcgc gccccagcgg gtgcggccgg tgcgggcgcc gccagcgcga aatcacgatc
    34861 gctgccgcca ccgacagcac ggcgttg
//
LOCUS       DAOQTZ010000013        34337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1457_length_34337_cov_14.329561, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000013 DAOQTZ010000000
VERSION     DAOQTZ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 34337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34337
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_34337_cov_14.329561"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..610
                     /locus_tag="PKN51_02975"
     CDS             68..610
                     /locus_tag="PKN51_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HNT84092.1"
                     /translation="MDDIVRQAMAKWPNVPHCHGWLGLDARGHWYLRDAQAQALGGFA
                     SGVAGARGTRLQHEGLIGFIQRNYAADAAGCWYFQNGPQRVFVELEVAPWVWRVASDG
                     AVSAHDGRPALVQAVWLDEQGRVFLRTDLGFGIVHTLDMGAVADAVEAGDWQPVAVHS
                     AELPQREGYVLSPQARSCRP"
     gene            complement(979..1842)
                     /locus_tag="PKN51_02980"
     CDS             complement(979..1842)
                     /locus_tag="PKN51_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNT84093.1"
                     /translation="MSDSTHDDAHTGPIKSPKQMFWVSVAAFVLPVFIIIGLVSFVTS
                     GNKTSPGASDMERAIAERLQKVGRVEIRDANRPLASGEEVYKTQCAACHATGVSGAPK
                     FQNAGDWGSRLGQGYAALLNSSLKGKGAMAPQGGGNFSDLEIGRAVVYMANAAGGKLA
                     EPAAPAAAASAADAAPAPAAAAAPAPAAAPAPAPVAAAAPAPAAAAGDAAAGKALYDK
                     ACVTCHAAGVAGAPKLGDKAAWAPRIAEGMDAMMKIAINGKGAMPPRGASTASDDELR
                     AAVQFMVDAAK"
     gene            complement(1978..3486)
                     /locus_tag="PKN51_02985"
     CDS             complement(1978..3486)
                     /locus_tag="PKN51_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNT84094.1"
                     /translation="MSHARHRLSRAPLVLAGLVAVGLLAGCSLVPPYERPAAPIASHW
                     PDTAGAPGADTTTAADLPWQQFVRDDQLRELIGLALTSNRDLRVAVQNIEQARAQFQI
                     RRADQFPSVGASASGTRSAPNPYQALGGPSVSSSYSVGLGVSAWELDFFGRVAALKDA
                     ALASYLATEEARKSSQISLIANVASTWLQLKTDTELLALAERTLGTREQSLALTRLRF
                     EHGAASALDLRQAETLIANARATRAQQQRLRAQDIHLLTLLVGQPLPERLIPAVPALP
                     TPQSPSEPTQASATVAPAAELPAFADVPAGLPSDVLLRRPDIRAAEQQLIAANAQIGA
                     ARANFFPRITLTGSLGRVSSDLDGLFGSGGSRAWSFGPAITLPIFDAGRNQAGLDSAL
                     AGRDIAVAQYEKAIQTAFREVADALAGRATLAEQLAALQAQAEAERARFQLTDLRYRN
                     GVASSLDLLDAQRSLFAIEQALAQARLAQRANEVQLYKALGGGWSESPAAGA"
     gene            complement(3483..6737)
                     /locus_tag="PKN51_02990"
     CDS             complement(3483..6737)
                     /locus_tag="PKN51_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT84095.1"
                     /translation="MARFFIDRPIFAWVIALFLMVIGATAITQLPIAQYPPVAPPTIV
                     VNAAYPGASAQTLEDSVLAVIEREMNGAPGLAYMESVAQADGTGQITISFEPGTNADL
                     AQVEVQNRLSRATPRLPAVVNQQGVRVDKARANFLMFVMLSSSNPDVDVTGLSDYAAR
                     NVQPELQRVPGIGQVQLFGSERAMRIWIDPDKLHSYKLAPSDVASAIQAQNAQVSAGA
                     IGNQPLVAGQTMTATVVVPGQLSTPEQFGAIVLRANADGSSVRLKDVARIELGAQSYA
                     SAARLNGEPGAGMGIQLSPTGNALAAAEAVRARMSELQAFFPPGVTWTIPYDASNFIK
                     ISIRKVVETLLEAVVLVFLVMFLFLQNWRYTLIPTIVVPVALLGTFGALLAFGMSINV
                     LTMFGMVLVIGIVVDDAIVVVENVERIMSEEGLPPLAATRKAMSQISGAIVGVTAVLI
                     AVFVPLAFFKGSTGNIYRQFAAVMGVSIFFSAFMALSLTPALCGTLLKPVQAGHQQAK
                     GGFFGWFNRGFARTTRGYEGVVARLLRRSGRLMLIYLLLAAVVGWMYVRLPTAFLPQE
                     DQGNLLVNIQLPPGATTERTQTVVEQIEGYLLKQPEVQSMVAVMGFSFSGQGQNAGIS
                     FVTLKPWDERTGPQHAADAVAGRAMGALSTIKDAFIFPLNPPPIPELGVSSGFSFRLQ
                     DRGGKGHDALLAARNQMLGMAMQSKLLAGVRPNGLEDAPQLQIDIDRDRAAAQGVSFG
                     AINSVISASLGSSYVNDFPNAGRLQRVVVQADGPARMTPDDVLRLTVLNNKGQAVPLS
                     ALASTRWVTGAMQTVRYNGYPAMRIDGGAAPGYSSGEAMAEMERLASRLPEGFGFEWT
                     GQSREEKLAGNQALVLYGFAILAVVLALAALYESWSIPLAVILVVPLGVLGVLLAVTG
                     RGLLNDVYFQVGLITIIGLSAKNAILIIEFAKDLQAQGKSALESALAAAHLRFRPIVM
                     TSLAFGLGVLPLAIASGASSASQRAIGTGVMGGIVVGTTLAVFFVPIFFVVVRSLFKP
                     NQRELERSQQHAEHAGITAASADAYVAAAEQGMSEAERQALHQGAQPPLTKDPS"
     gene            complement(6750..8039)
                     /locus_tag="PKN51_02995"
     CDS             complement(6750..8039)
                     /locus_tag="PKN51_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT84096.1"
                     /translation="MTSKQTVSRTWARRLAPVGIAAALTLTLAACGKGQPPAAGAGGP
                     GGGMPPPEVAVLTVQPGVVPISTELPGRLEASRVAQVRARVTGILQRRVFTEGSDVKA
                     GQTLFRIDAAPYEATLQSARAQLAQAQAQLAQTSATAARYKPLVAANAVSRQEYDAAV
                     AAEKAAQAQVAAGQAAVRTASINLGYATVTAPIAGRIGRALVTEGALVNQQQDGGTPL
                     AVIQQIDPLYINITQSANQVLQLREALAAGQLARASGAEAAKVQVLLDNGQPFGQSGR
                     LLFTDLSVDPATGEVSLRAELPNPGGRLLPGMYVRVRIEQAQVEGAILLPQQAVTRGS
                     QGDSVLVVAADGSVAPRPITIRGAQGQHWIVTGGLQPGEQVMVEGAMKLMLGAKVVKP
                     VPWQPKSAAAPDGQAPAATKTEATKAPASAPAGAASN"
     gene            8281..8931
                     /locus_tag="PKN51_03000"
     CDS             8281..8931
                     /locus_tag="PKN51_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNT84097.1"
                     /translation="MARRTKEDALATRAALLDAAECVFQQRGVSRTSLADIAQAAGVT
                     RGALYWHFKDKAALFNAMMDRVTLPLGAELEGLLEQRDDPLQALCQHLDEALHQIVHD
                     EQTRRVLRIAMQKVEHAEELGPVIDRHIQMHRLNTERERQVFERAAALRQCVLPAPAA
                     QLAHGFYAMVHGLIYSWLLDEGFDLQATAAVSVQAFLRGIGLVPAQAAPSPAADGR"
     gene            8951..9026
                     /locus_tag="PKN51_03005"
     tRNA            8951..9026
                     /locus_tag="PKN51_03005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8984..8986,aa:Arg,seq:ccg)
     gene            complement(9120..9602)
                     /locus_tag="PKN51_03010"
     CDS             complement(9120..9602)
                     /locus_tag="PKN51_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HNT84098.1"
                     /translation="MNTPRINIGISEQDRAAIAQGLSRLLADTYTLYLTTHNFHWNVT
                     GPMFNTLHAMFMAQYTELWNAVDPIAERIRSLGHVAPGSYAAFGKLSSLADAPPEPPR
                     AMDMVRLLVQGHEAVARTARELFPQADAAGDEPTADLLTQRMTVHEQTAWMLRSLLEE
                     "
     gene            complement(9647..11176)
                     /locus_tag="PKN51_03015"
     CDS             complement(9647..11176)
                     /locus_tag="PKN51_03015"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNT84099.1"
                     /translation="MSQDSRRCPVTHLTTDFGAPVPTNRDSLTAGPRGPLLAQDVWLN
                     EKLANFVREVIPERRMHAKGSGAFGTFTVTHDITRYTRAKIFEKVGKKTEMFARFTTV
                     AGERGAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFIRDPRQFPDLNKAVKRDPRT
                     NMRSATHNWDYWTLLPEALHQITIVMSERGIPASYRHMHGFGSHTYSFWNAKGERFWV
                     KFHFKTQQGIKNLTDAEAEALIGRDRESHQRDLYEAIARGDFPKWTMYVQVMPELDAD
                     KVAYHPFDLTKIWPHADYPLIEVGEFVLNKNPENFFADVEQSAFAPSNLVPGIGVSPD
                     RMLQARLTNYADAQRYRLGTNHHQIPVNAARCPVHSNHRDGMGRVDGNYGGALHYEPN
                     SFGQWQAQPQFAEPPLRLSGDAQHFRFHEDDANYFEQPRKLFQLMSDAQKQALFGNTA
                     RAMGDAPEFVKQRHIRNCHAADPAYAAGVAQALGLDLQKALASQKDDPMFGNPLVALP
                     V"
     gene            complement(11559..13511)
                     /gene="mrdA"
                     /locus_tag="PKN51_03020"
     CDS             complement(11559..13511)
                     /gene="mrdA"
                     /locus_tag="PKN51_03020"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNT84100.1"
                     /translation="MTELRNVEAELMRFRIRLAVVALAVLLAFGLLGWRLYQLQVARH
                     DELARQAESNRTAVVPVVPHRGQITDRHGVVLATNYKAYTLEITPSRTGRGIDDTIDA
                     IAQVIEVTPRDRRRFKRLREDSRSFESLPIRTRLTDEEVARFAAQRYRFPGVEIKARL
                     FRNYPLGETGAHVIGYIGRINPKEKERIEDSDDAANYRGTDHIGKLGIEASYEKLLHG
                     TTGWEQMETSAGGRAVRRLGSHPATPGEAIRLTIDIHLQKLVEDMYGERRGALVAIDP
                     TNGEVLAFVSKPSFDPNLFVEGIDHESWDELNNSIDKPLLNRGLRGTYPPGSTYKPFM
                     GLAALETGKRAPGSVTMDGGSWTFGGHTFRSGHALGPVDLHRSIVKSSNVYYYQLAND
                     MGVNAIHDFMKPLGFGQITGIDLPGEARGVLPSTDWKRTTYRRPEQQKWYAGETISLG
                     IGQGYNNFTILQLASATATLAAGGVRHVPHLVKARQDAVSLQLHELPQPAAQPLGYRP
                     EHVALVRRAMVGVTQEGTSRAVFAGAPYLSGGKTGTAQAVTIGQKSRYNAAKLAEHQR
                     DHSLYIAFAPAEQPRIAVAAIVENAGFGAAHAAPLVRRVMDYWLAGVYPSEADIAALQ
                     RGQASAPIGTPRRVAEVTAAAPSAAP"
     gene            complement(13693..14211)
                     /gene="mreD"
                     /locus_tag="PKN51_03025"
     CDS             complement(13693..14211)
                     /gene="mreD"
                     /locus_tag="PKN51_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNT84101.1"
                     /translation="MILPRGQPLLLPAHPGFIWGSLLLALMLDLLPLGRVPWMPDFLA
                     VVLVFWSIHQPRRVGLSAAFVFGLVLDVHQASLLGQHALAYAVLIYLAIMVHRRILWF
                     AVPAQALQMLPLFAAAHAITLVLRLIGGGIFPGWSVLLAPLLEALMWPIVSWLLLAPQ
                     RRAPNPDANRPL"
     gene            complement(14208..15227)
                     /gene="mreC"
                     /locus_tag="PKN51_03030"
     CDS             complement(14208..15227)
                     /gene="mreC"
                     /locus_tag="PKN51_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNT84102.1"
                     /translation="MPLDTLDRTPPPFFKQGPSALSRLVFFSALALFLMVADARFHIV
                     QPLRAAIATVLYPAQWLALQPVQMVRQLTGYMTDLKTARATEEAARRKLAEQSERAGQ
                     VEQLLLENTRLRELLELRQRVQAPGMAAQVLYDATDPYSRRVVIDKGQLQGVQPGSPV
                     LDESGVLGQVTRVYPFVSEITLLIDREQVIPVMNARTGTRSVAYGDPAPHGGAMELRY
                     MSVNEDVKEDDLLVTSGVDGVYAPGLPVARVTTVDRRSDTSFARIRCQPLARLQGVTH
                     VMVLAPLTPQVAEGPTGAATADSGAPTAASAPAQASAPASAAASAAASAPAAPRRRAA
                     TGGRP"
     gene            complement(15408..16451)
                     /locus_tag="PKN51_03035"
     CDS             complement(15408..16451)
                     /locus_tag="PKN51_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNT84103.1"
                     /translation="MFGALRRYFSTDLAIDLGTANTLIYVRDKGIVLDEPSVVAIRHE
                     GGPQGKKTIQAVGREAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPR
                     GMFKPSPRIIICVPCGSTQVERRAIRESALGAGASEVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSARVGGDKFDDAIINYIRRNYGMLIGDPTAEL
                     IKKEIGSAFPGSEVREMEVKGRNLSEGVPRSFTISSNEILEALTEPLNNIVSEVKKAL
                     EETPPELGADIAERGMMLTGGGALLRDLERLLAEETGLPVLVAEEPLTCVVRGCGLAL
                     ERMERLGSIFTSE"
     gene            16652..16951
                     /gene="gatC"
                     /locus_tag="PKN51_03040"
     CDS             16652..16951
                     /gene="gatC"
                     /locus_tag="PKN51_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNT84104.1"
                     /translation="MALTSDDIRHLAHLARLELNAEQSERMLTSLNSFFAIAQAMQAE
                     DTRGVAPLAHPLAAVQDVQLRLRDDVVSEPDRREAYQRNAPAVEDGLFLVPKVIE"
     gene            16948..18411
                     /gene="gatA"
                     /locus_tag="PKN51_03045"
     CDS             16948..18411
                     /gene="gatA"
                     /locus_tag="PKN51_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNT84105.1"
                     /translation="MSALHDLTVAEAARRLRARQLSALELARHLLARSQAHAGLGAFL
                     AQDEDVTLAQARAADARLAAGDAPPLTGVPVAHKDVFVTTDFPSTAGSRILAGYRSPF
                     ESTVTARLAEAGMVCLGKLNCDEFAMGSSNENSAFGPVRNPWDTTRVPGGSSGGSAAA
                     VAARLVPAATGSDTGGSIRQPSSFCGLTGIKPTYGRVSRYGMIAYASSFDQAGPMARS
                     AEDCALLLSSLCGPDLDRDSTSLELPPEDFTRQLGASIEGLRIGIPREFFGDGLAPDV
                     RAALDGALAELCKLGARLVDIRLPRTELSIPVYYILTPAEASTNLSRFDGVRYGHRAP
                     EYADLEELYKKSRSQGFGEEVQRRIIIGTYVLSHGYYDAYYLQAQKLRRMIADDLQRA
                     FGECDVIAGPVSPGVAWPLGAHSADPLADYLADIYTLTASMAGLPAMSVPAGFGAGGM
                     PVGLQLIGNYLQEARLLNVAHRLQQATDWHTRKPQGY"
     gene            18425..19897
                     /gene="gatB"
                     /locus_tag="PKN51_03050"
     CDS             18425..19897
                     /gene="gatB"
                     /locus_tag="PKN51_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNT84106.1"
                     /translation="MMSTLIQGYEVVIGFETHAQLRTRSKLFSRAATAFGAAPNTQAS
                     AVDLALPGTLPVMNREAVRCAIRLGLALGSRIAPRSVFDRKNYFYPDLPKGYQISQFQ
                     QPVVQGGEVSFFVGPSTGSGQAQEKKTVRLVRAHLEEDAGKSLHEDFHGMSGIDLNRA
                     GTPLLEIVTEPDMRSSAEAVAYARELHKIVTWIGICDGNMQEGSFRCDANVSVRKPGQ
                     PLGTRREIKNLNSFKFMQQAIDYEIRWQIEQLEDGHAIQQATVLFDPDSGETRAMRTK
                     EDSADYRYFPDPDLPPLCISESWIEQERAQMPELPRQMAERLVAQHGLPEYDAATLTQ
                     SPAMAAYFEQAAAASGQPKLASNWVMGELSRRLNAEDIGIEAARVSAAQLAQLIGRIQ
                     DGTISNAAARQVFEVLWRGEGETVDAVIEAKGLRQMNDSAALEKIVDEVIAANPGNVE
                     QFKAGKDKAFNALVGQVMKASRGKANPQQASELLRARLGA"
     gene            complement(19964..20677)
                     /locus_tag="PKN51_03055"
     CDS             complement(19964..20677)
                     /locus_tag="PKN51_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNT84107.1"
                     /translation="MNASPAAARSPAATGAAWRWRALAAGALLLGGGVGALAQGGGAA
                     PSSGIYTCIDAHGRRLTSDRPIAACIDREQRELNRDGTVRRVIPPTLSATEREALEAR
                     EREAALKRQRERDLLRRDQALLTRYPDQAAHDAGRAEELAQTQQVIQAAQQRIAELGQ
                     ERKALDDEMEFYVRDPARAPAKLRRALTDNAEAVAAQRRAIAAQQEEQRRIHARFDAE
                     ALRLQTLWPTARAGDAPKR"
     gene            complement(20674..21369)
                     /gene="pyrE"
                     /locus_tag="PKN51_03060"
     CDS             complement(20674..21369)
                     /gene="pyrE"
                     /locus_tag="PKN51_03060"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNT84108.1"
                     /translation="MSVTLPTRDEQNRLAQEFVQFAIESGVLRFGEFKTKAGRLSPYF
                     FNAGLFDDGAKLGRLAQFYARALLASGIEFDLIFGPAYKGIPLGAAVAIELARLGVNK
                     PFAYNRKEAKDHGEGGTLVGAPLAGRVLIVDDVISAGTAVRESIALIQAAGATPHAVA
                     IALDRQEKATENGQDVPWSAVQYVRETLGLQVCAIASLSDLLQHLSHTAHFSAHYDAV
                     SAYRARYGVTQTT"
     gene            21383..22174
                     /locus_tag="PKN51_03065"
     CDS             21383..22174
                     /locus_tag="PKN51_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNT84109.1"
                     /translation="MFKLTSLNLNGIRSAATKGVQAWLEAHAPDCICVQEIKAQSPDL
                     AGRFESLAGLRGHFHLAEKKGYSGVGLYTRHEPSAVQAGFGSPEFDAEGRYLEARFDT
                     PRRRFSLISAYFPSGSSGPERQQAKFRFLAEFYPHLQRLKRERDFILCGDVNIAHQEI
                     DLKNWKSNQKNSGFLPEERAWMSQVLDELQLVDVYRRLHPDTTGDSYTWWSNRGQAYA
                     KNVGWRLDYHLATPALGACARAAAVYKAQKFSDHAPITIDYAFSL"
     gene            22252..23322
                     /locus_tag="PKN51_03070"
     CDS             22252..23322
                     /locus_tag="PKN51_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase"
                     /protein_id="HNT84110.1"
                     /translation="MSKALRLSEKWFQRGLWLVALVFASFLIGLGSTLVGDLPRVESV
                     PALDDFMDRQAAQAARDSVQAGERQAQDHADALEQARLKLQVAQSDVGAQRQSFANWI
                     ATRRATERAEQDPELLARTRALDELHQRERAARAELERQQQALLDARQSVEQARRTLA
                     DMQRNAQGAYEQALRVQELRVFGYRLALTLPLLALAGWLFARQRQSRWWPFVWGFILF
                     AVFVFFVELVPYLPSYGGYVRYTVGIVATVLVGRWAIVALQRYLERQRAAEAQPDAQR
                     RAELGYDTALARLAKGVCPGCERTVDLKDGRTDFCPHCGIGLFDRCGPCQARKSAFAR
                     FCPACGSSAASPSPAAMALPHN"
     gene            complement(23396..24370)
                     /locus_tag="PKN51_03075"
     CDS             complement(23396..24370)
                     /locus_tag="PKN51_03075"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNT84111.1"
                     /translation="MKTRITELFGIEHPIIQGGMHYVGFAELAAAVSNAGGLGVITGL
                     TQKTPEQLAREIARCRDMTDRPFGVNLTFLPTVSSPDYPGYIRAIIEGGVKAVETAGN
                     NPQQWLPLLKEHGVKVIHKCTSVRHALKAEAIGCDAVSVDGFECGGHPGEDDVPNFIL
                     LPRAAETLKIPFVASGGMADGRSLVAALALGAEGMNMGTRFIATQEAPVHEHVKQAIV
                     AASELDTRLVMRPLRNTERVLTNAAVERLLAKERQLGASLKFEDIIDEVAGVYPRIMQ
                     TGDMDAGAWSCGMVAGLIHDIPTVKALIDRIMAEAEALITQRLAGLVR"
     gene            complement(24465..25637)
                     /gene="tgt"
                     /locus_tag="PKN51_03080"
     CDS             complement(24465..25637)
                     /gene="tgt"
                     /locus_tag="PKN51_03080"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNT84112.1"
                     /translation="MLRFELLGQDPSSHARRGRLTLNHGVVETPIFMPVGTYGTVKGV
                     LPRSLREMGAQIILGNTFHLWMRPGLDVLRQFGGLHGFENWHQPILTDSGGFQVWSLG
                     EMRKISEEGVKFASPVNGDRLFLTPEISMQIQTVLNSDIVMQFDECTPYETRGHVTTE
                     AEARASMALSLRWARRSQAEFERLGNPNALFGIVQGGMFEQLREESLAQLVELDLPGY
                     AIGGVSVGEPKEDMLRITAHTPHRLPAHKPRYLMGVGTPEDLVYGVQHGVDMFDCVMP
                     TRNARNGHLFTRFGDLKIRNARHKSDPRPIDESCTCHTCAGASGVAWQDGGREGFSRA
                     YLHHLERCGEMLAPMLASIHNLHYYLNLMRDIRAALDAGAFGAFRQRFAAERARGV"
     gene            complement(25645..26970)
                     /locus_tag="PKN51_03085"
     CDS             complement(25645..26970)
                     /locus_tag="PKN51_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT84113.1"
                     /translation="MLASTPTADPFTLQLQQIRGLIEAHQLPQAAQALNAAQQQAPKD
                     ARIPLLGMRLAERAGNLERATQAARRALALAPDWHVAQIELALLLARQKQGDEAISLA
                     MRALASAPQEAQVMVGAINVALSAERHEQTLKWAEDGVRRFPESAGIRLFLARFLAAQ
                     GRLADATTHYEFILQRLPHHEETLQGLLSCALSMDDQARARTYAERLLALKPFDENAR
                     YWHAVVHGQTPPTQPAQVVTGIFDGYADQFDVHLVRGLQYRVPERVAQILLEHHPDRH
                     FNLLDLGCGTGLVGVYLGRIEGHIIGVDLSEKMIEQAARHQLYSRFHRVNVLDALRET
                     PAEHYEAITCTDVLVYVGDLAPVIPNALRILKPGGHFIFSCEAAAEDEADLVLRPSQR
                     YAHKASAVERQCREAGFDDIRIEHLPTLRMENDQPLPGFLVTARKPPAA"
     gene            complement(27259..28389)
                     /gene="queA"
                     /locus_tag="PKN51_03090"
     CDS             complement(27259..28389)
                     /gene="queA"
                     /locus_tag="PKN51_03090"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNT84114.1"
                     /translation="MSDRTFTTSDFDFALPPELIAQHPAPERSASRLLDGSGAAPIDR
                     VFRDLPALLQPGDLLVFNDTQVVKARLYGHKPPAPLARSRRSPQGAPIGLGRPGADRQ
                     PSGGKLELLVERVLPDGSVAAHMKVSKKPPPGTVLRMAGDFTATVLGRWPQADGPLFH
                     LRLSGDAYQLMAAHGHVPLPPYIQHTDSAQDVARYQTVFARHPGAVAAPTAALHFDDA
                     LLAAIDARGVQRAFVTLHVGAGTFQPVRTENLAEHLMHRERYLVPPATAQAVAECRAR
                     GGRVVAVGTTSVRTLESWAASGQRQGDTDIFITPGFGFRVVDALVTNFHLPRSTLMML
                     VSALAGHDHVMALYAHAVAARYRFFSYGDAMWLTRRDGEPAR"
     gene            28443..30545
                     /gene="recG"
                     /locus_tag="PKN51_03095"
     CDS             28443..30545
                     /gene="recG"
                     /locus_tag="PKN51_03095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNT84115.1"
                     /translation="MPATEATAAPAAERALSAPQKALRKLGLVRPIDLALHLPLRYED
                     ETHLTPLAEAARTLSDAPLQFEATVVHHEVAYRPRRQLLVTVEDGGERCLLRFFNFYP
                     SQLKQMAVGTRLRVRGELRGGFGGLAMMHPVVRAAGGALPQALTPIYPTVAGLPQPYL
                     RKAVLGGLEQADLSDTLPDEVLSEIGHLRPWDLRKALSFLHQPTPDTALAQLEDHSHP
                     AWQRLKAEELLAQQLSQLRARRARQHLRAPVLPAQRGGLHEALLAALPFQLTGAQRRV
                     GEEIARDLARPQPMHRLLQGDVGSGKTAVAALAACIAIDAGWQCALMAPTEILAEQHF
                     TKLADWLAPLLAPRGRQVAWLTGGQKKKARADMLAAIESGAAALVIGTHAVIQQQVRF
                     ARLGLAVIDEQHRFGVQQRLDLRNKLSEAEQEPHLLMMSATPIPRTLAMSYYADLDVS
                     TLDELPPGRTPVITRLVAQARRGEVIERIRAQIAQGRQVYWVCPLIEESEALDLRHAS
                     ATHAELAAALPGQAVGLLHSRLPPADKQAVMQQFVSGAMPVLVSTTVIEVGVDVPNAS
                     LMVIEHAERFGLSQLHQLRGRVGRGAAASACVLLYATPEGGRLGDSARERLKAMVETS
                     DGFEIARRDLAIRGPGEFLGARQSGDALLRFADLAEDAPLLAWARRAAPRLLDHWPER
                     AARHVDRWLGGRAEYLKA"
     gene            30716..31678
                     /locus_tag="PKN51_03100"
     CDS             30716..31678
                     /locus_tag="PKN51_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNT84116.1"
                     /translation="MTLTELKYIVAVAREKHFGKAAEACFVSQPTLSVAVKKLEEELE
                     VKLFERGGSEIAVTPLGAEIVRQAQSVLEQAAEIKEIAKRGKDPLGGPLTLGVIYTIG
                     PYLLPELVRHNIERTPQMPLMLQENFTVRLLEMLRTGEIDCAILAEPFPDSGLAVAPL
                     YDEPFMAALPAAHPLARGAAVTSEQLRGEHMLLLGTGHCFRDHVLQVCPEFAGFASSG
                     EGIRRNFEGSSLETIKHMVAAGMGVTLVPRLAVPVEALVAQAAQADAPGAVRYLPVRD
                     GGGAAPPTRRVVLAWRRSFTRYEAIAALRNAIYACELPGVQRLS"
     gene            31748..32623
                     /gene="ubiA"
                     /locus_tag="PKN51_03105"
     CDS             31748..32623
                     /gene="ubiA"
                     /locus_tag="PKN51_03105"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNT84117.1"
                     /translation="MTGSPSSPPRSKWSLYLDLIRWDRPAGWLVLLWPTLSALWLAAG
                     GFPGWHLLAVFVAGTILMRSAGCCVNDVADREFDRHVKRTAQRPVTTGAVGVREALAL
                     GAVLALAAFVLVLTTNASAIALSFPALAIAIAYPFAKRWVSMPQAVLGVAFSFGIPMA
                     FAAVQGQVPWQAWALLAGNLFWVLAYDTEYAMVDRDDDLKIGIQTSAITLGRWDVAAI
                     MGFYAAYLSIWIVIVLQQAPTAIVYIAFGLAAGQALWHYTLIRGRTREGCFTAFRQNH
                     WLGATVFAGIALALR"
     gene            complement(32632..33426)
                     /locus_tag="PKN51_03110"
     CDS             complement(32632..33426)
                     /locus_tag="PKN51_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNT84118.1"
                     /translation="MPTLSRRQFAALGLVGLTIPPVWAQVKDMNDAINKAGRQRMLSQ
                     RVAKAYLGVALEALPAQAEKVLSQSMALFDRQLVELKGFAPNPGIRDTYGQLERAWLD
                     FKDRLVGKPASMAGADAVLAQSETVLALAHQGTTQLEKASGQPSGRLVNLAGRQRMLS
                     QRLAKYALASGARIQLATATTEIEKNRSEFVTGLEVLAEAPQATPRIRETLKLGEQQW
                     LLFDAAIRSSSRSGQPVKEALAHIMTTSENLLLVLDDVTNQYAALR"
     gene            complement(33641..>34337)
                     /gene="proC"
                     /locus_tag="PKN51_03115"
     CDS             complement(33641..>34337)
                     /gene="proC"
                     /locus_tag="PKN51_03115"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNT84119.1"
                     /translation="TRAALLQQHGVTALPAAGPQLAGADLIVWAVKPQSFGAAAAPVA
                     PHVGSALQLSVMAGIRSGDIAAASGARRIVRCMPNTPALVGRGMTGLFAAPEVSAADR
                     ALAEAVIRTTGEALWLEHEAQLDAVTALSGSGPAYVFYFLEAMQQAGSELGLSPEQAR
                     RLAVATFAGGAHLAAASSEPLATLRERVTSKGGTTYAALTALDQAGVKPAFVAAMHAA
                     CARAKELGDEFGR"
BASE COUNT         4981 a        11606 c        12329 g         5421 t
ORIGIN      
        1 tgcccgaaga tgacaagcgg tcaacggcct tctctgggtt cgaaggccgc ttcaataaga
       61 tagcggcatg gacgacatcg tgcgccaggc catggccaag tggccgaacg tgccgcactg
      121 ccacggctgg ctggggctgg acgcacgcgg ccactggtac ctgcgcgatg cccaggcgca
      181 ggccctgggg ggctttgcca gcggcgtggc cggcgcgcgc ggcacgcgct tgcagcacga
      241 aggcctgatc ggcttcattc agcgcaacta cgccgccgac gcggccggct gctggtattt
      301 ccagaacggg ccgcagcgcg tattcgtcga gctggaagtg gcgccctggg tgtggcgtgt
      361 ggcgtccgac ggcgccgtgt cggcgcacga cggccgtccg gcactggtgc aggcggtgtg
      421 gctggacgag caggggcgcg tgtttctgcg caccgacctg ggctttggca tcgtccacac
      481 gctggacatg ggcgccgtgg ccgacgcggt ggaagccggc gactggcagc ccgtcgcggt
      541 gcacagcgcc gagctgccgc agcgcgaagg ctatgtactg agcccgcagg cgcgatcgtg
      601 caggccctga gcagggcgac cggcactatc aaaaatgtag ctatcggcgc ttgccgatcg
      661 ggtgccagag gcctgagcct ggaaccggtg gttgactgct tgtcaacttc gggcaggccg
      721 cgtccaagca gggtttggcg actgcgatgg cctacaccgg ctgggagaga gcgccacaca
      781 tccacccgca ccgcgctcgc cgcgccatgc ggcgttgctc ccccttgcgg gcacgagtcc
      841 gcggcggcgt cgcgtcttgc ctggcacgcc gatcacggca cgggcgggcg cgtccaactg
      901 ccgcttttgg gctgaatgaa ccaaaaagaa agccgctgaa gcagcggctt ttctagcggg
      961 ttcagggttg cgggccgatc acttggcggc gtccaccatg aactgcacgg cggcgcgcag
     1021 ctcgtcgtcg ctggcggtgg aggcgccgcg tggcggcatg gcgcccttgc cgttgatggc
     1081 gatcttcatc atggcgtcca tgccttcggc aatgcgcggc gcccaggccg ccttgtcgcc
     1141 cagcttgggc gcgccggcca caccggctgc gtggcaggtg acgcaggcct tgtcgtacag
     1201 ggccttgccg gcggccgcat cgcccgcggc ggcggccggt gccggtgctg ccgcggccac
     1261 cggtgcgggg gccggtgcag cggcgggggc gggtgcggca gccgcggcag gcgccggagc
     1321 ggcgtcggcc gcactggcgg ctgcagccgg ggcggccggc tcggccagtt tgccgccggc
     1381 agcgttggcc atgtagacca cggcgcgccc gatctccagg tcactgaagt tgccgccacc
     1441 ctgcggcgcc atggcgccct tgcctttgag cgacgagttc agcagcgccg cgtagccctg
     1501 gcccaggcgc gagccccagt cgccggcgtt ctggaacttc ggcgcgcccg agacgccggt
     1561 ggcgtggcag gcggcgcatt gcgtcttgta gacctcttcg ccgctggcca gcgggcggtt
     1621 ggcgtcgcgg atttccactc ggcccacctt ctgcaggcgt tcggcgatgg cgcgttccat
     1681 gtcggacgcg cccggcgagg tcttgttgcc cgaggtgacg aaggacacca ggccgatgat
     1741 gatgaacacc ggcagcacga aggccgccac actgacccag aacatctgct tgggtgactt
     1801 gatgggtccg gtatgggcgt cgtcgtgcgt gctgtcgctc atggcttcct ctggcgggcg
     1861 gtcggggttg tctcgtggtt gggtgcgcgt cacggcgcgg gctgcgggcg cgccgtgcgt
     1921 cgggcgtgac aaaaatcaat tatagggaca gcgtgtcacg cccctcaggc cttgccctca
     1981 ggcccctgcg gccggcgact cgctccagcc gccgcccagc gccttgtaga gctgcacctc
     2041 gttggcgcgc tgggccagtc gtgcctgggc cagcgcctgt tcgatggcaa acagcgaacg
     2101 ctgggcgtcc agcaggtcga gcgagctggc cacgccgttg cggtagcgca gatcggtcag
     2161 ctgaaagcgc gcgcgctcgg cctcggcctg cgcttgcagc gcggccagct gctcggccag
     2221 cgtggcgcgg ccggccaggg catcggcgac ctcgcggaag gcggtctgga tggctttctc
     2281 gtactgcgcc acggcgatgt cccgcccggc cagggcgcta tcgagcccgg cctggttgcg
     2341 cccggcgtcg aagatcggca gcgtgatggc ggggccgaac gaccaggccc tgctgccgcc
     2401 ggagccgaac aggccatcca ggtcgctgct gacgcgcccc aggctgccgg tcagcgtgat
     2461 gcgcggaaag aaattggcgc gtgcggcacc gatctgggcg ttggcggcga tcagctgctg
     2521 ctcggcggcg cggatgtcgg gccggcgcag cagcacgtcg gacggcaggc cggccggcac
     2581 gtcggcaaag gcaggcagct cggccgccgg cgccacggtg gcgctggcct gggtcggctc
     2641 gctcgggctc tggggcgtgg gcagggccgg gacggcgggg atgagccgct cgggcagggg
     2701 ctgacccacc agcagcgtca gcaggtggat gtcctgcgcg cgcaggcgct gctgctgggc
     2761 ccgcgtggcg cgggcgttgg cgatcagggt ctcggcctgg cgcagatcca gcgccgaggc
     2821 ggcgccgtgc tcgaagcgca gccgcgtcag cgccagcgac tgctcgcgcg tgcccagcgt
     2881 acgctcggcc agcgccagca gctcggtgtc ggtcttcagt tgcagccagg tgctggccac
     2941 gttggcgatc aggctgatct gggacgactt gcgcgcctcc tcggtggcca ggtagctggc
     3001 cagcgcggcg tccttgagcg cggccacgcg gccgaagaaa tcgagttccc aggccgacac
     3061 gcccagcccc acgctgtagc tgctgctcac gctgggcccg cccagcgcct ggtaggggtt
     3121 gggggccgag cgcgtgccgc tggcgctggc gcccacgctg gggaactggt cggcgcgccg
     3181 gatctggaac tgggcacgcg cctgttcgat gttctgtacc gccacccgca ggtcgcggtt
     3241 gctggtcagg gccagcccga tcagctcgcg cagctggtcg tcgcgcacga actgctgcca
     3301 gggcaggtcg gcggcggtcg tggtgtcggc gccgggcgcg ccggcggtgt cgggccagtg
     3361 gctggcgatg ggggccgccg gacgttcgta gggcggcacc agcgagcagc cggccagcag
     3421 gccgaccgcc accaggccgg ccagcaccag cggggcgcgg gagaggcgat gacgggcgtg
     3481 actcatgatg gatccttggt gagcggcggt tgcgcgccct ggtgcagcgc ctggcgttcg
     3541 gcttcgctca tgccctgttc agcggccgcg acgtaggcgt cggccgaggc ggcggtgatg
     3601 ccggcgtgct cggcgtgttg ctgcgagcgc tccagctcgc gctggttggg cttgaacagg
     3661 ctgcgcacga cgacgaagaa gatcggcacg aaaaacaccg ccagcgtggt gcccaccaca
     3721 atgccgccca tcaccccggt gccgatggcg cgctgactgg ccgagctggc gccgctggcg
     3781 atggccagcg gcagcacgcc caggccgaac gccagcgagg tcatgacgat ggggcgaaag
     3841 cgcaggtggg cagcggccag cgccgactcc agcgcgctct tgccctgcgc ctgcaggtcc
     3901 ttggcgaact cgatgatcag gatggcgttc ttggccgaca ggccgatgat ggtgatcagg
     3961 ccgacctgga aatacacgtc gttgagcagg ccgcgcccgg tgacggccag cagcacgccc
     4021 agcacgccca gcggcaccac caggatcacc gccagcggga tcgaccagct ctcgtacagc
     4081 gccgccagcg ccagcaccac cgccaggatg gcgaagccgt acagcaccag cgcctggttg
     4141 ccggccagct tttcctcgcg cgactggccg gtccattcga agccgaagcc ctcgggcagg
     4201 cggctggcca ggcgctccat ttcggccatc gcctcgcccg agctgtagcc cggcgccgcg
     4261 ccaccgtcga tgcgcatggc cgggtagccg ttgtagcgca cggtctgcat ggcgccggtg
     4321 acccagcgcg tgctggccag cgccgacagc ggcaccgcct gtcccttgtt gttgagcacc
     4381 gtcaggcgca gcacgtcgtc gggcgtcatg cgcgccggtc cgtcggcctg caccaccacg
     4441 cgctgcaggc ggccggcgtt cgggaagtcg ttgacgtagc tcgaccccag cgaggccgag
     4501 atgacgctgt tgatggcgcc gaaactcacg ccctgcgcgg ccgcgcggtc gcggtcgatg
     4561 tcgatctgca gctgcggcgc gtcctccagg ccgttcgggc gcacaccggc cagcagcttg
     4621 ctctgcatgg ccatgcccag catctggttg cgcgccgcca gcagggcgtc atggcccttg
     4681 ccaccgcggt cctgcagccg gaagctgaag cccgagctga cgcccagctc cgggatcggc
     4741 ggcgggttga gcgggaagat gaaggcatcc ttgatggtcg acagcgcgcc catggcgcgg
     4801 cctgccacgg catcggcggc gtgctgcggg ccggtgcgct cgtcccaggg cttgagggtg
     4861 acgaaggaaa tgccggcgtt ctggccctgg ccggagaacg agaagcccat caccgccacc
     4921 atgctctgca cttcgggctg cttgagcagg tagccctcga tctgctccac cacggtctgc
     4981 gtgcgctcgg tggtggcgcc aggcggcagc tggatgttga ccagcaggtt gccctggtct
     5041 tcctgcggca ggaaggcggt cggcaggcgc acgtacatcc agccgaccac ggccgccagc
     5101 agcaggtaga tcagcatcag ccggccgctg cgcctcagca ggcgcgcgac cacgccttcg
     5161 tagccgcgcg tggtgcgcgc aaagccgcgg ttgaaccagc cgaagaagcc gcccttggcc
     5221 tgctggtggc cggcctgtac cggcttgagc agcgtgccgc acagcgccgg cgtgagcgac
     5281 agcgccatga aggccgagaa gaagatcgac acccccatca cggcggcgaa ctggcggtag
     5341 atgttgccgg tcgagccctt gaagaacgcc agcggcacga acaccgcgat cagcaccgcc
     5401 gtcacgccca cgatggcgcc ggagatctgg ctcatggcct tgcgcgtggc ggccagcggc
     5461 ggcaggccct cttcgctcat gatgcgctcg acgttttcga ccaccacgat ggcgtcgtcc
     5521 accacgatgc cgatcaccag caccatgccg aacatggtca gcacgttgat cgacatgccg
     5581 aacgccagca gggcgccgaa ggtgcccagc agcgccaccg gcaccacgat ggtggggatc
     5641 agggtgtagc gccagttctg caggaacagg aacatcacca ggaacaccag caccaccgct
     5701 tccagcagcg tctcgaccac cttgcggatc gagatcttga tgaagttgga ggcgtcgtag
     5761 gggatggtcc aggtcacgcc gggcgggaag aacgcctgca gctcgctcat gcgcgcgcgc
     5821 acggcctcgg ccgccgccag ggcgttgccg gtgggcgaca gctggatgcc catgccggcg
     5881 ccgggttcgc cgttcaggcg cgcggcgctg gcgtagctct gcgcccccag ctcgatgcgc
     5941 gccacgtcct tcaggcgcac gctggagccg tcggcgttgg cgcgcagcac gatggcgccg
     6001 aactgctccg gcgtggacag ctggcccggc accaccacgg tggcggtcat ggtctggccc
     6061 gccaccagcg gctggttgcc gatggcaccg gccgacacct gcgcgttctg cgcctgaatg
     6121 gcgctggcca cgtcggacgg cgccagcttg tagctgtgca gcttgtccgg gtcgatccag
     6181 atgcgcatgg cgcgctcgga accgaacagc tgcacctggc cgatgcccgg cacgcgctgc
     6241 agctcgggct gcacgttgcg cgcggcgtag tcggacagcc cggtgacgtc gacgtccggg
     6301 ttggacgagg acagcatcac gaacatcagg aagttggcgc gcgccttgtc cacccgcacg
     6361 ccctgctggt tgaccacggc gggcaggcgc ggggtggcgc gcgacaggcg gttctgcacc
     6421 tcgacctggg ccaggtcggc gttggtgccg ggctcgaagc tgatggtgat ctggccggtg
     6481 ccgtcggcct gtgccaccga ttccatgtag gccaggccgg gcgcgccgtt catctcgcgc
     6541 tcgatcacgg ccagcaccga gtcttccagc gtctgcgccg aggcgcccgg gtaggcggcg
     6601 ttgaccacga tggtcggcgg cgccaccggc gggtactgcg cgatcggcaa ctgggtgatg
     6661 gcggtggcgc cgatcaccat caggaacagg gcaatcaccc acgcgaagat gggtcggtca
     6721 atgaaaaaac gtgccatgcc gggcgcctct cagttcgacg cggcgcccgc cggtgccgag
     6781 gccggggctt tggttgcttc tgttttggta gctgccggcg cttgtccatc gggcgccgca
     6841 gccgattttg gctgccatgg cacaggtttg accaccttgg cgcccagcat cagcttcatg
     6901 gcgccttcga ccatcacctg ctcacccggc tgcaggccac cggtgacgat ccagtgctgg
     6961 ccctgggcgc cgcggatggt gatcgggcgc ggcgccacgc tgccgtcggc cgccaccacc
     7021 agcaccgagt cgccctggct gccgcgcgtg accgcctgct gcggcagcag gatggcgcct
     7081 tccacctgcg cctgctcgat gcgcacccgc acgtacatgc cgggcagcag ccggccgccc
     7141 gggttgggca gttcggcgcg caggctgact tcgccggtcg cggggtcgac gctgaggtcg
     7201 gtgaacagca gccgcccgct ctggccgaac ggctggccgt tgtccagcag cacctgcacc
     7261 ttggccgctt cggcgccgct ggcgcgcgcc agctggccgg ccgccagggc ctcgcgcagc
     7321 tgcagcacct ggttggccga ctgggtgatg ttgatgtaga gcggatcgat ctgctgaatc
     7381 accgccaacg gcgtgccgcc gtcctgctgt tggttgacca gcgcgccttc ggtcaccagc
     7441 gcgcggccga tgcggccggc aatcggcgcc gtcacggtgg cgtagccgag gttgatgctg
     7501 gccgtgcgca ccgccgcctg accggccgcc acctgggcct gcgcggcttt ttcggccgcc
     7561 acggcggcgt cgtattcctg ccggctgacc gcgttggccg ccaccagcgg cttgtagcgc
     7621 gcagcggtcg cgctggtctg cgccagctgc gcctgcgctt gcgccagctg ggcccgggcg
     7681 ctctgcagcg tcgcttcata gggggcggcg tcgatgcgga acagcgtctg cccggccttc
     7741 acatcgctgc cttcggtgaa cacgcgccgc tgcaggatgc cggtgacgcg cgcgcgcacc
     7801 tgggccacgc gcgaggcctc cagccggccc ggcagctcgg tgctgatcgg caccacgccc
     7861 ggctgcacag tgagcaccgc cacctcgggc ggcggcatgc caccgcccgg cccgccggcg
     7921 ccggcggccg ggggctggcc tttgccgcag gcggccagcg tcagggtcag cgcggcggcg
     7981 atgccgacgg gcgccaggcg gcgcgcccag gtgcgggaga cggtttgctt tgaggtcatg
     8041 aggcttgttc tttgcgacgg agggatcggc aggcacggcc gtcagcgcca cgccatgatg
     8101 agcggcggcc accgaagcgg ttgagcgggc ggcaccactt ctgggggcgg cgccggcacg
     8161 gcgtcgggga caactgacga tggcgcgaat tatacataca ttcttgaatg tatgcgctac
     8221 aatggatgcc gtgccgatcg ctgacgagca gcgattcttc ccgtttttcc ggttttctgc
     8281 atggcccgac gcaccaagga ggatgccctc gcaacccgtg ccgcgctgct ggacgcggcc
     8341 gagtgcgtgt ttcagcagcg cggggtgtca cgtacctcgc tggccgacat cgcccaggcc
     8401 gcgggggtca cgcgcggcgc gctgtactgg cacttcaagg acaaggcggc gctgttcaac
     8461 gccatgatgg accgcgtcac gctgccgctg ggcgccgagt tggagggcct gctggagcag
     8521 cgcgacgatc cgctgcaggc gctgtgtcag catctggacg aggcgctgca ccagatcgtg
     8581 cacgacgagc agacgcggcg cgtgttgcgc atcgccatgc agaaggtcga gcatgccgag
     8641 gaactgggcc cggtgatcga tcgccacatc cagatgcacc gcctgaacac cgagcgcgag
     8701 cgccaggtgt tcgagcgcgc ggcggcgctg cgccagtgcg tgttgccggc cccggcggcg
     8761 cagctggcgc acggcttcta cgccatggtg cacgggctga tctacagctg gctgctcgac
     8821 gaagggtttg acctgcaagc caccgccgcg gtgtcggtgc aggcctttct gcgcggcatc
     8881 ggcctggtgc cggcgcaggc cgcgccgtca ccggcggccg atgggcgata atgcccggct
     8941 tgcattgcac gcggctgtag ctcagtggat agagtatcgg cctccgaagc cgagggtcgt
     9001 gggttcgatc cccgccagcc gcgccagatc atcaagaaag ccttggccag cgtcagggct
     9061 tttttgttga ttgctactta gttaatagct gcttgcgcac gctggtcggg cgctggcgcc
     9121 tattcttcca gtaaggaacg cagcatccag gcggtctgct cgtgcaccgt catgcgctgg
     9181 gtcagcaggt cggccgtcgg ttcgtcgccg gcggcgtcgg cctgcgggaa cagctcgcgc
     9241 gcggtgcgcg ccacggcctc atggccctgc accagcaggc gcaccatatc catggcgcgc
     9301 ggtggctcgg gcggcgcatc cgccaacgac gacagcttgc cgaaggcggc gtaggaaccg
     9361 ggagccacat ggccgagcga gcggatgcgc tcggcgatcg gatcgacagc gttccacagc
     9421 tcggtgtact gcgccatgaa catggcgtgc agggtgttga acatcggccc ggtgacgttc
     9481 cagtggaagt tgtgcgtggt caggtacagc gtgtaggtgt cggccagcag gcgcgacagg
     9541 ccctgggcga tggcggcgcg atcctgttcg ctgatgccga tgttgatgcg gggcgtgttc
     9601 atggcgaatc tcctgaagtc gatgggatgg tgacggtggc gaccgctcac acgggcagcg
     9661 ccaccagcgg gttgccgaac atggggtcgt ccttctgcga ggccagggcc ttttgcagat
     9721 ccaggcccag cgcctgggcc acgccggcgg cgtaggccgg gtcggccgcg tggcagttgc
     9781 ggatgtggcg ctgcttgacg aactcgggcg catcgcccat ggcgcgcgcg gtgttgccaa
     9841 acagcgcctg tttttgcgcg tcgctcatca gttggaagag tttgcgcggc tgctcaaagt
     9901 agttggcgtc gtcctcgtgg aagcggaagt gctgggcatc gcccgacagg cgcagcggcg
     9961 gctcggcaaa ctggggctgg gcctgccact ggccaaagct gttgggctcg tagtgcaggg
    10021 cgccgccgta gttgccgtcc acccggccca tgccgtcgcg gtggttgctg tgcacggggc
    10081 agcgcgcggc gttcaccggg atctggtggt gattcgtgcc caggcggtag cgctgcgcgt
    10141 cggcgtagtt ggtcaggcgc gcctgcagca tgcggtcggg gctcacgcct atgccgggca
    10201 ccaggttgct gggggcaaac gcggactgct ccacgtcagc gaagaagttc tcggggttct
    10261 tgttgagcac gaactcgccc acctcgatca gcgggtagtc ggcgtgcggc cagatcttgg
    10321 tcaggtcgaa cgggtggtag gccaccttgt cggcatcgag ctcgggcatg acctgcacat
    10381 acatggtcca cttggggaaa tcgccgcgtg ctatggcttc gtagagatcg cgctggtggc
    10441 tctcgcggtc acggccgatg agcgcctcgg cctcggcgtc cgtgaggttc ttgatgccct
    10501 gctgcgtctt gaagtggaac ttgacccaga agcgctcgcc ctttgcattc cagaagctgt
    10561 aggtgtgcga gccaaagccg tgcatgtggc ggtagctcgc cggaatgccg cgctcgctca
    10621 tgacgatggt gatctggtgc aaggcctcgg gcaagagcgt ccagtagtcc cagttgtgcg
    10681 tggcgctgcg catgttggtg cgcgggtcgc gcttgacggc cttgttcagg tcggggaact
    10741 ggcgcgggtc gcggatgaag aacaccggcg tgttgttgcc caccatgtcc cagttgccct
    10801 cctcggtata gaacttgagg gcaaagccgc ggatgtcgcg ctcggcgtcg gccgcaccgc
    10861 gctcgcccgc gacggtggtg aagcgggcga acatctcggt cttcttgccg accttctcaa
    10921 agatcttggc gcgcgtgtag cgcgtgatgt cgtgcgtgac cgtgaaggtg ccgaacgcac
    10981 ccgagccctt ggcatgcatg cgccgctcgg gaatcacctc gcgcacgaag ttggcgagtt
    11041 tttcgttcag ccacacatcc tgcgccagca gcgggccgcg tgggccagcg gtgaggctgt
    11101 cacgattggt cggcacgggg gcgccgaagt cggtggtcag gtgggtgacg gggcagcggc
    11161 gcgaatcttg gctcatggtt gactccttgg aggaaatggg tggggcggtg gatgactgag
    11221 gtcatgatag gtcgcatgca ttgatatgaa caatcaatag tttcaatcaa ggcattaggc
    11281 cggttcaacc tagaaacggc agtgcatagc gccttcaccc agaaggtgaa ggctcccaag
    11341 tggtgcaagg tcagagttca tgcggcacct ccaagggttg caagcggtca actgcggttt
    11401 ctaggttcaa cgcagttgga ggcgctcgca ttcgatcggt gcgccagagc acagccacgc
    11461 gacgccatgc cggcgcgtca gtggtggctt cgggcagcgg tgaccggcag gcatcgggcc
    11521 ggtgcgccgc cgttggggcg gccgggccgc cggcggcgtc agggcgctgc gctgggcgcg
    11581 gcggcggtga cttcggccac gcgccgcggc gtcccgatgg gggcgctggc ctggccgcgc
    11641 tgcagcgccg cgatgtcggc ttcgctcggg tagacgccgg ccagccagta gtccatcacg
    11701 cggcgcacca gcggcgcggc gtgggcggcc ccgaagccgg cgttttcgac gatggccgcc
    11761 accgcaatgc gcggctgttc ggccggcgca aaggcgatgt agagcgagtg gtcgcgctgg
    11821 tgctcggcca gcttggcggc gttgtagcgg ctcttctgcc cgatggtcac cgcctgcgcg
    11881 gtgccggtct tgccgcccga gaggtagggc gcaccggcga acaccgcgcg cgaggtgccc
    11941 tcctgcgtca cgccgaccat ggcacgcctg accagcgcca cgtgctcggg ccggtagccc
    12001 agcggctggg cagccggctg cggcagctca tgcagctgca ggctcaccgc gtcctggcgg
    12061 gccttgacca ggtgcggcac atggcgcacg ccgccggccg ccagcgtggc ggtggcgctg
    12121 gccagctgca ggatggtgaa gttgttgtag ccctggccga tgcccagcga gatggtttcg
    12181 ccggcgtacc acttctgctg ctcggggcgc cggtaggtgg tgcgcttcca gtcggtcgag
    12241 ggcagcacgc cgcgcgcttc gccaggcagg tcgatgccgg tgatctggcc aaaacccagc
    12301 ggcttcatga agtcgtggat ggcattgacg cccatgtcgt tggccagctg gtagtagtac
    12361 acgttgctgg acttgacgat gctgcggtgc aggtccaccg gcccgagcgc gtggccggaa
    12421 cggaaggtgt ggccgccgaa ggtccaggag ccgccgtcca tggtgacgct gcccggggcg
    12481 cgcttgccgg tttccagcgc cgccagtccc atgaagggct tgtaggtcga gccgggcggg
    12541 taggtgccgc gcaggccgcg gttgagcagg ggcttgtcga tggagttgtt gagttcgtcc
    12601 cagctttcgt ggtcgatccc ttcgacgaac aggttggggt cgaagctcgg cttgctgacg
    12661 aaggccagca cttcgccgtt ggtcgggtcg atcgccacca gcgcgccgcg gcgctcgccg
    12721 tacatgtctt cgacgagctt ttgcaggtgg atgtcgatgg tcagccggat ggcctcgccc
    12781 ggggtggcgg ggtggctgcc caggcgacgc acggcgcggc cgccggccga ggtctccatc
    12841 tgctcccagc cggtggtgcc gtgcagcagc ttttcgtagc tggcctcgat gccgagcttg
    12901 ccgatgtggt cggtgccgcg gtagttggcc gcgtcgtccg aatcctcgat gcgctccttt
    12961 tccttcgggt tgatgcggcc gatgtagccg atcacatggg cgccggtttc gcccagcggg
    13021 tagttgcgaa acagccgcgc cttgatttcg accccgggga agcggtagcg ttgggcggca
    13081 aagcgcgcca cctcctcgtc ggtcaggcgg gtgcggatcg gcagcgattc gaagctgcgc
    13141 gaatcctcgc gcaggcgctt gaagcggcgc cggtcgcgtg gcgtgacttc gatcacctgg
    13201 gcgatcgcgt cgatggtgtc gtcgatgccg cggccggtgc gcgaaggtgt gatctccagc
    13261 gtgtaggcct tgtagttggt ggccagcacc acgccgtggc ggtcggtgat ctggccgcgg
    13321 tgcggcacca ccggcaccac cgccgtgcgg ttggattcgg cctgacgcgc cagttcgtcg
    13381 tggcgcgcca cctgcagttg gtacaggcgc cagcccagca ggccgaaggc cagcagcacc
    13441 gccagcgcga ccaccgccag ccggatgcga aagcgcatca gctcggcttc gacgttgcgc
    13501 agttcggtca tggtgccgcc tgcctcgcgg gcgcaggccc gccgtgccgc cgagcgtgtg
    13561 ggccgcccgg ggcccggtgt gccagggtcg cgcgcctcca gtgccgtgcc atcatgtccc
    13621 gaatgctatt aaatgcatag ctgcttgcgc ttgccagata agcgcgaaag cggtatttga
    13681 ccacgaaacc acctacagcg ggcgattggc gtccgggttg ggcgcgcggc gctgcggcgc
    13741 cagcagcagc cagctgacga tcggccacat cagggcctcc agcagcggcg ccagcagcac
    13801 cgaccagccc ggaaagatgc cgccgccgat cagccgcagc accagggtga tggcgtgcgc
    13861 cgccgcgaac agcggcagca tctgcagcgc ctgcgccggc accgcgaacc acaggatgcg
    13921 ccggtgcacc atgatggcca ggtagatcag caccgcgtag gccagcgcgt gctggcccag
    13981 cagcgaagcc tggtgcacgt ccagcaccag tccgaacacg aaggccgcgc tcaggcccac
    14041 gcggcgtggc tggtggatgc tccagaacac cagcaccacc gccaggaagt caggcatcca
    14101 tggcacccgg cccagcggca gcaggtcgag catcagcgcc agcagcaggc tgccccagat
    14161 gaacccgggg tgggccggca gcagcagggg ttgaccgcgc ggcaggatca tgggcggcct
    14221 ccggtggcgg cgcgccggcg cggcgcggcg ggggcggaag ccgctgccga ggcggctgcc
    14281 gaagcgggcg ccgaggcctg ggcgggtgcg ctggcggccg tcggcgcgcc ggagtcggcg
    14341 gtggcggcgc cggtcggccc ctcggcgacc tggggcgtca gcggcgccag caccatcacg
    14401 tgcgtcaccc cctgcagccg cgccagcggc tggcagcgga tgcgggcaaa cgaggtgtcg
    14461 ctgcgccggt ccaccgtggt cacgcgcgcc accggcaggc cgggcgcata cacgccgtcc
    14521 acgccactgg tcaccagcag atcgtcttcc ttgacgtcct cgttgaccga catgtagcgc
    14581 agctccatgg cgccgccgtg cggcgccggg tcaccataag ccacgctgcg cgtgccggtg
    14641 cgtgcgttca tcaccgggat cacctgctcg cggtcgatca gcagggtgat ttcgctgacg
    14701 aaggggtaga cgcgcgtgac ctggcccagc acgccggact cgtccagcac cggcgagccc
    14761 ggctgcacgc cctgcagctg gcccttgtcg atcaccacgc gccggctgta gggatcggtg
    14821 gcgtcgtaca gcacctgcgc cgccatgccc ggcgcctgca cgcgctggcg cagctccagc
    14881 agctcgcgca ggcgggtgtt ttccagcagc agctgctcga cctgaccggc gcgctcggac
    14941 tgctcggcca gtttgcgccg cgccgcctcc tcggtggcgc gcgcggtttt caggtcggtc
    15001 atgtagccgg tcagctgccg caccatctgc accggctgca gcgccagcca ttgggccggg
    15061 tagagcacgg tggcgatggc cgcgcgcagc ggctgcacga tgtgaaagcg cgcgtcggcc
    15121 accatcagga acagcgccag cgcactgaag aagaccagcc gcgacagcgc cgacggccct
    15181 tgcttgaaga agggcggcgg cgtgcggtcg agcgtgtcga gcggcatggc gggtcaggcc
    15241 gacatcggtc gggacagacg cgatgctaca ttaataagag ctatttgcgc acgccggtaa
    15301 agcgccagaa gcagtttttg catgaagcgg cggagcgtgc gggcggcgtc ggcggcaggc
    15361 cggggcgtgg cggcgcgccg gccacctgcg gccaccggga cgcggcgtca ttcgctggtg
    15421 aagatgctgc ccagccgctc catgcgctcc agcgccaggc cgcagccgcg caccacgcag
    15481 gtcagcggct cctcggccac cagcaccggc aggccggttt cctcggccag cagacgctcc
    15541 aggtcgcgca gcagggcgcc gccgccggtc agcatcatgc cgcgctcggc gatgtcggcg
    15601 cccagctcgg gcggggtttc ctccagcgcc ttcttgactt cggagacgat gttgttgagc
    15661 ggctcggtga gcgcttcgag gatctcgttg ctgctgatgg tgaagctgcg cggcacgccc
    15721 tcggacaggt tgcggccctt gacctccatc tcgcgcacct cgctgcccgg gaaggcgctg
    15781 ccgatttcct tcttgatcag ctcggccgtc gggtcgccga tcagcatgcc gtagttgcgg
    15841 cggatgtagt tgatgatggc gtcgtcgaac ttgtcgccgc ccacgcgggc gctgcccttg
    15901 tagaccatgc cgcccaggct gatcacgccg acttcggtgg tgccgccgcc gatgtccacc
    15961 accatcgagc cgctggcctc gctcaccggc aggccggcgc cgatggccgc ggccatgggt
    16021 tcttcgatca gatagacctc gctggcgccc gcgcccaggg ccgattcgcg aatggcgcgg
    16081 cgctcgacct gggtcgagcc gcagggcacg cagatgatga tgcgcgggct gggcttgaac
    16141 atgccgcgcg ggtgcaccat cttgatgaac tgcttgagca tctgctcggt gacggtgaag
    16201 tcggcgatca cgccgtcctt catcgggcgg atcgcctcga tgttgcccgg caccttgccc
    16261 agcatggcct tggcctcgcg gcccacggcc tggatggtct tcttgccctg cgggccacct
    16321 tcgtgacgga tcgcaaccac cgacggctcg tccagcacga tgcccttgtc gcgcacgtag
    16381 atcagggtgt tggcggtgcc gaggtcgatg gccaggtcgg tggagaagta ccgacggaga
    16441 gctccaaaca tgaaaaaccc ttctgggccg ccggccagca ccagacgccg gcaaccgctg
    16501 aatcaatgcg aaatgagcgt gatttcgcgc aaatggcgcc caattccggg ccgttgcgcc
    16561 aaaggcggga taatagcgca tccccgtcgc cggcccctgc ggcgcacccg ccgcggcagc
    16621 cgggctcctg ctgttttttc gtgccttttt catggccctg acatctgatg acatccgcca
    16681 cctcgcccat ctggcgcggc tcgagctgaa tgccgaacaa agtgagcgca tgcttacaag
    16741 cttgaactcg ttctttgcca tcgcccaggc gatgcaggcc gaggacaccc gcggtgtggc
    16801 gccgctggcg cacccgctgg ccgccgtcca ggacgtgcag ctgcgcctgc gcgacgacgt
    16861 ggtgagcgag cccgaccggc gcgaggccta ccagcgcaac gcgccggcgg tggaggacgg
    16921 cctgtttctg gtgccgaagg tgatcgaatg agcgcgctgc acgacctgac cgtggccgag
    16981 gcggcccgcc ggctgcgcgc gcgccagctg tcggcgctgg agctggcccg gcacctgctg
    17041 gcgcgcagcc aggcgcatgc cgggctgggc gcctttctgg cgcaggacga ggacgtgacc
    17101 ctggcgcagg cgcgcgcggc cgacgcccgg ctggcggccg gcgacgctcc accgctcacc
    17161 ggcgtgccgg tggcgcacaa ggacgtgttc gtcaccaccg acttccccag caccgccggt
    17221 tcgcgcatcc tggccggcta tcgatcgccg ttcgagagca cggtgacggc gcggctggcc
    17281 gaggccggca tggtctgcct gggcaagctc aactgcgacg aattcgccat gggctcgtcc
    17341 aacgagaact cggccttcgg tccggtgcgc aacccctggg acaccacgcg cgtgccgggc
    17401 ggttcttcgg gcggcagcgc cgcggcggtg gcggcgcgcc tggtgccggc ggccaccggc
    17461 agcgacaccg gcggctcgat ccgccagccc tccagctttt gtggcctgac cggcatcaaa
    17521 cccacctacg ggcgggtgtc gcgctacggc atgatcgcct acgcctccag cttcgaccag
    17581 gccggcccga tggcgcgcag cgccgaagac tgcgcgctgc tgctgtccag cctgtgcggc
    17641 cccgacctgg accgcgactc gacctcgctc gagctgccgc ccgaggactt cacgcgccag
    17701 ctgggcgcgt cgatcgaagg cctgcgcatc ggcattccgc gcgagttctt cggcgacggc
    17761 ctggcgcccg acgtgcgcgc cgcgctggat ggcgcgctgg ccgagctgtg caagctgggc
    17821 gcgcggctgg tggacatccg cctgccgcgc accgagctgt cgattccggt ctactacatc
    17881 ctcacgccgg ccgaggccag caccaacctg agccgcttcg acggcgtgcg ctacggccac
    17941 cgcgcgcccg aatacgccga cctggaggag ctgtacaaga agagccgcag ccagggcttt
    18001 ggcgaagaag tgcagcggcg catcatcatc ggcacctacg tgctgtcgca cggctactac
    18061 gacgcctact acctgcaggc gcagaagctg cgccgcatga tcgccgacga cctgcagcgc
    18121 gccttcggcg aatgcgacgt catcgccggc ccggtgtcgc ccggcgtggc ctggccgctg
    18181 ggcgcgcaca gcgccgaccc gctggccgac tacctggccg acatctacac cctgaccgcc
    18241 tccatggccg gtctgccggc catgagcgtg ccggccggct ttggcgccgg gggcatgccg
    18301 gtcggtctgc agctgatcgg caactacctg caggaagcgc gtctgctgaa cgtggcgcac
    18361 cgcctgcagc aggccaccga ctggcacacc cgcaagcccc agggttattg aagaggggcc
    18421 gcgcatcatg agcacactga tccagggcta cgaagtcgtc atcggcttcg agacgcacgc
    18481 ccagctgcgc acgcgcagca agctgttttc acgcgccgcc acggcgttcg gcgccgcccc
    18541 caacacccag gccagcgcgg tcgatctggc gctgcccggc acgctgccgg tgatgaaccg
    18601 cgaggcggtg cgctgcgcca tccggctcgg gctggcgctg ggctcgcgca ttgcgccgcg
    18661 cagcgtgttc gaccgcaaga actacttcta ccccgacctg cccaagggct accagatcag
    18721 ccagttccag cagccggtgg tgcagggcgg cgaagtgtcg ttcttcgtcg gcccttcgac
    18781 aggctcagga caggctcaag agaaaaagac cgtgcgcctg gtgcgcgccc acctggaaga
    18841 agacgccggc aagtcgctgc acgaggactt tcacggcatg agcggcatcg atctgaaccg
    18901 cgccggcacg ccgctgctgg agatcgtcac cgagcccgac atgcgcagca gcgccgaggc
    18961 cgtggcctac gcgcgcgagc tgcacaagat cgtcacctgg atcggtatct gcgacggcaa
    19021 catgcaggaa ggcagctttc gctgcgacgc caacgtatcc gtgcgcaagc ccggccagcc
    19081 gctgggcacg cggcgcgaga tcaagaacct caacagcttc aagttcatgc agcaggcgat
    19141 cgactacgag atccgctggc agatcgagca gctggaagac ggccacgcga tccagcaggc
    19201 caccgtgctg ttcgaccctg acagcggcga gacgcgcgcc atgcgcacca aggaagactc
    19261 ggccgactac cgctacttcc ccgaccctga cctgccgccg ctgtgcattt cggagtcatg
    19321 gatcgaacag gagcgcgccc agatgcccga gctgccgcgc cagatggcgg agcgcctggt
    19381 ggcccagcat ggcctgcccg aatacgacgc cgcgaccctg acgcagtcgc ccgccatggc
    19441 cgcctacttc gagcaggcgg cggccgccag cggtcagccc aagctggcca gcaactgggt
    19501 gatgggcgaa ctttcgcggc gcctgaacgc cgaggacatc ggcatcgaag cggcgcgcgt
    19561 gtcggcggcg cagctggcgc agctgatcgg ccgcatccag gacggcacca tcagcaacgc
    19621 cgccgcgcgc caggtgttcg aagtgctgtg gcgcggcgag ggcgagacgg tggacgccgt
    19681 gatcgaggcc aagggcctca ggcagatgaa cgacagcgcg gcgctggaga agatcgtcga
    19741 cgaggtgatc gccgccaacc ccggcaacgt cgagcagttc aaggccggca aggacaaggc
    19801 cttcaacgcc ctggtcggcc aggtgatgaa agccagccgc ggcaaggcca acccacagca
    19861 ggccagcgag ctgctgcggg cccgcctggg tgcttgacga aagctatatt tataatagct
    19921 gctgacgcag tctggacggg cgccagcgga tgatttggtc gaatcagcgc ttgggcgcat
    19981 cgcccgcgcg ggcggtcggc cacagcgtct gcaggcgcag cgcctcggcg tcgaagcggg
    20041 cgtggatgcg gcgctgctct tcctgctggg cggcgatggc gcggcgctgg gccgccaccg
    20101 cctcggcgtt gtcggtcagc gcgcggcgca gcttggccgg ggcgcgggcc gggtcgcgga
    20161 cgtagaactc catctcgtcg tccagcgcct tgcgctcctg gcccagctcg gcgatgcgct
    20221 gctgtgccgc ctggatcacc tgctgggtct gcgccagctc ttcggcgcgc ccggcgtcgt
    20281 gcgcggcctg gtcggggtag cgggtgagca gcgcctgatc gcggcgcagc agatcgcgtt
    20341 cgcgctggcg cttgagcgcc gcttcgcgct cgcgcgcttc gagggcctcg cgctcggtgg
    20401 cgctgagcgt gggtgggatg acgcgccgca cggtgccgtc gcggttgagc tcgcgctgct
    20461 cgcggtcgat gcaggccgcg attgggcggt ccgacgtcag gcgccggcca tgggcgtcga
    20521 tgcaggtgta gatgccactg gatggcgccg cgccaccacc ctgggccagc gcgccaacgc
    20581 caccccccag cagcagcgca ccggccgcca gcgcacgcca ccgccatgcc gcgccggtgg
    20641 cggcaggcga gcgggcagct gctggcgatg cgttcatgtc gtctgtgtaa cgccgtaccg
    20701 tgcccgatag gccgacacgg cgtcgtaatg cgctgagaag tgtgcagtat gtgacaagtg
    20761 ttgcagaaga tccgacaggc tggcgatggc gcagacctgc agccccagcg tttcgcgcac
    20821 gtactgcacg gccgaccagg gcacgtcctg gccgttttcg gtggcctttt cctgacgatc
    20881 cagcgcgatc gccaccgcgt gcggcgtggc gccggcggcc tggatgaggg caatcgattc
    20941 gcgcaccgcg gtgccggcgg agatcacgtc gtcgacgatc agcacccgac cggccagcgg
    21001 cgcgccgacc agggtgccgc cttcgccgtg gtccttggct tccttgcggt tgtaggcaaa
    21061 cggcttgttg acgcccaggc gcgccagctc gatggcgacc gccgcgccca gcggaatgcc
    21121 cttgtaggcc gggccgaaga tgaggtcgaa ctcgatgccc gacgccagca gggcgcgcgc
    21181 atagaattgc gccagccggc ccagcttggc gccgtcgtcg aacaggccgg cattgaagaa
    21241 atagggcgac aggcgccctg ccttggtctt gaattcgcca aagcgcagca cgccgctttc
    21301 gatggcgaat tgcacgaatt cctgcgccag ccggttttgc tcgtcacggg ttggcagtgt
    21361 cacgctcatg ggggtccgtt ccttgttcaa gctcaccagc ctcaacctca acggcatccg
    21421 ctcggccgcc accaagggcg tgcaggcctg gctggaggcg cacgcgccgg attgtatttg
    21481 tgtgcaggag atcaaggccc agtcgcccga tctggccggc cgcttcgagt cgctggccgg
    21541 gctgcgcggg cactttcatc tggccgagaa gaaaggctat tcgggcgtcg gcctctacac
    21601 ccgccatgaa ccgagcgccg tgcaggccgg cttcggctcg cccgagttcg acgccgaggg
    21661 ccgttatctg gaggcgcgtt tcgacacccc gcggcgccgg ttttcgctca tcagcgccta
    21721 ctttcccagt ggctcctccg gccccgagcg gcagcaggcc aagttccgct ttctggccga
    21781 gttctacccg cacctgcagc gcctcaagcg cgagcgcgac ttcatcctgt gcggcgacgt
    21841 caacatcgcg caccaggaaa tcgacctcaa aaactggaag agcaaccaga agaacagcgg
    21901 ttttctgccc gaggagcgcg cctggatgag ccaggtgctc gatgaactgc aactggtgga
    21961 cgtctaccgc cgcctgcacc ccgacaccac cggcgattcc tacacctggt ggagcaaccg
    22021 cggccaggct tatgccaaga acgtgggctg gcggctggac taccacctgg ccacgccggc
    22081 gctcggtgcg tgtgcgcgag cggcggcggt gtacaaggcg cagaagttca gcgaccacgc
    22141 gcccatcacc atcgactacg cgttttccct ttgagagcgc tatgttaaat atagctgccg
    22201 gcgcttgttg gtagcgcgcc gttgccgtta ttgcttgaga atggacgtga gatgagcaaa
    22261 gccctgcgcc tgtcggagaa atggtttcag cgcggtctgt ggctggtggc cctggtgttt
    22321 gccagcttcc tgatcggcct gggcagcacc ctggtgggcg atctgccgcg ggtcgagagc
    22381 gtgccggcgc tggacgactt catggaccgt caggccgccc aggccgcgcg cgacagcgtg
    22441 caggccggcg aacgccaggc gcaggaccac gccgacgcgc tggagcaggc gcggctgaag
    22501 ctgcaggtgg cgcagtccga cgtcggcgcc cagcggcaga gctttgccaa ctggattgcc
    22561 acccgccgcg ccaccgagcg cgccgagcag gaccccgagc tgctggcccg cacccgtgcg
    22621 ctggacgaac tgcaccagcg cgagcgcgcc gcccgcgccg agctggagcg gcagcagcag
    22681 gcgttgctgg atgcgcgcca gtcggtcgag caggcgcgcc gcacgctggc ggacatgcag
    22741 cgcaacgccc agggcgccta cgaacaggcg ctgcgggtgc aggagctgcg cgtgttcggc
    22801 taccggctgg cgctgacgct gccgctgctg gcgctggccg gctggttgtt cgcccgtcag
    22861 cggcagagcc gctggtggcc cttcgtgtgg ggcttcatcc tgtttgcggt gttcgtgttc
    22921 ttcgtcgagc tggtgcccta cctgcccagc tacggcggct atgtgcgcta cacggtcggc
    22981 atcgtcgcca ccgtgctggt ggggcgctgg gccatcgtgg cgctgcagcg ctacctggag
    23041 cgtcagcgcg cggccgaggc ccaaccggac gcgcagcgcc gcgccgaact gggctacgac
    23101 acggcgctgg cgcgcctggc caagggcgtg tgcccgggct gcgagcgcac ggtggacctg
    23161 aaggacggca ggaccgactt ctgcccgcac tgcggcatcg gtctgttcga tcgctgtggc
    23221 ccctgccagg cgcgcaagag cgcgtttgcc cgcttctgcc ccgcctgtgg cagcagcgcc
    23281 gccagcccat cgccggccgc gatggcgctg ccgcacaact agattgctac aattaaagta
    23341 gctaatggcg cttgctggac aagcgccgga tggcgattgg gcgctggata cgggctcagc
    23401 gcaccaaccc cgccaggcgc tgcgtgatca gcgcctcggc ctcggccatg atgcggtcga
    23461 tcagcgcctt cacggtcggg atgtcgtgga tcaggccggc caccatgccg cagctccagg
    23521 cgccggcgtc catgtcgccg gtctgcatga tgcgcgggta gacgccggcc acttcgtcga
    23581 tgatgtcctc gaacttcagg ctggcgccga gctgcctttc cttggccagc aggcgctcga
    23641 cggcggcgtt ggtcagcacg cgctcggtgt tgcgcagcgg gcgcatcacc agccgggtgt
    23701 ccagctcgct ggcggcgacg atggcctgct tgacgtgctc gtgcaccggc gcttcctggg
    23761 tggcgatgaa gcgcgtgccc atgttcatgc cctcggcgcc cagggccagc gccgccacca
    23821 ggctgcgccc gtcggccatg cctcccgagg cgacgaaggg gatcttcagc gtctcggcgg
    23881 cgcgcggcag caggatgaag ttgggcacgt cgtcctcgcc cgggtggccg ccgcactcga
    23941 agccgtcgac actgacggcg tcgcagccga tggcctcggc cttcagcgcg tggcgcaccg
    24001 aggtgcattt gtggatcacc ttgacgccgt gctccttcag cagcggcagc cactgctgcg
    24061 ggttgttgcc cgccgtctcc accgccttca cgccgccttc gatgatggcg cggatgtagc
    24121 ccgggtagtc gggcgagctg acggtgggca gaaaggtgag gttgacgccg aagggccggt
    24181 cggtcatgtc gcggcagcgc gcgatctcgc gcgccagctg ctcgggcgtc ttctgcgtca
    24241 ggccggtgat gacgcccagc cccccggcgt tggagacggc ggcggccagc tcggcaaagc
    24301 cgacgtagtg catgccgccc tggatgatgg ggtgttcaat gccgaacagc tcggtgatgc
    24361 gggtcttcat ggtggaggct ccggaaaggg tgggggtcag ggggcagagc cgcgcagcat
    24421 aggcgcggcg tggcgccgcc gcagtcacct gggcgccacg gccatcagac gccgcgcgcg
    24481 cgttcggcgg cgaagcgctg gcggaaggcg ccgaacgcgc cggcatcgag cgcggcgcga
    24541 atgtcgcgca tcaggttcag gtagtagtgc aggttgtgga tggacgccag catcggcgcc
    24601 agcatctcgc cgcagcgctc cagatggtgc aggtaggcgc gcgaaaagcc ctcgcgcccg
    24661 ccgtcctgcc aggccacccc gctggcgccg gcgcaggtgt ggcaggtgca gctctcgtcg
    24721 atcgggcgcg ggtcgctctt gtggcgggcg ttgcggatct tcaggtcgcc gaagcgcgtg
    24781 aacaggtggc cgttgcgcgc gttgcgcgtg ggcatcacgc agtcgaacat gtccaccccg
    24841 tgctgcacgc cgtacaccag gtcttccggc gtgcccacgc ccatcaggta gcgcggcttg
    24901 tgcgccggca ggcggtgcgg cgtgtgggcg gtgatgcgca gcatgtcttc cttgggttcg
    24961 cccacgctga cgccgccgat ggcgtagccg ggcaggtcca gctccaccag ctgcgccagc
    25021 gattcttcgc gcagctgctc gaacatcccg ccctgtacga tgccgaacag cgcgttgggg
    25081 ttgcccaggc gctcgaactc ggcctggctg cggcgtgccc agcgcaggct gagcgccatg
    25141 ctggcgcgcg cttcggcctc ggtggtgacg tggccgcgcg tctcgtaggg cgtacattcg
    25201 tcgaactgca tgacgatgtc gctgttcagc acggtctgga tctgcatgct gatctcgggc
    25261 gtgagaaaca gccggtcgcc gttgaccggg ctggcgaatt tcacgccctc ctcgctgatc
    25321 ttgcgcatct cgcccagcga ccagacctga aagccgcccg agtcggtcag gatgggttgg
    25381 tgccagttct cgaagccgtg cagaccgccg aactgccgca gcacgtccag cccgggccgc
    25441 atccacaggt ggaaggtgtt gcccaggatg atctgcgcgc ccatctcgcg caggctgcgc
    25501 ggcagcaccc ccttgacggt gccgtaggtg ccgacgggca tgaagatcgg cgtctcgacg
    25561 acgccgtggt tcagcgtcag gcgtccgcgg cgcgcgtggc tggacgggtc ctggcccagc
    25621 agttcgaagc gcagcatggc tgcgtcaggc cgccggcggc ttgcgcgccg tcaccagaaa
    25681 gcccggcagc ggctggtcgt tttccatgcg cagcgtgggc aggtgttcga tgcggatgtc
    25741 gtcgaagccg gcttcgcggc actggcgctc gaccgcgctg gccttgtggg cgtagcgctg
    25801 gctgggacgc agcaccaggt cggcttcgtc ctcggcggcg gcctcgcacg agaagatgaa
    25861 atgcccgccg ggtttgagga tgcgcagcgc gttgggaatc accggcgcca gatcgcccac
    25921 gtagaccagc acgtcggtgc aggtgatggc ttcgtagtgc tcggccggcg tttcgcgcag
    25981 ggcgtcgagc acgttcacgc gatgaaagcg cgaatagagc tggtggcgtg cggcttgttc
    26041 gatcatcttt tccgacagat cgacgccgat gatgtgccct tcgatgcggc ccaggtagac
    26101 acccaccagc ccagtgccgc agcccaggtc gagcaggttg aagtggcggt ccgggtgatg
    26161 ctccaacagg atttgagcca cgcgctcggg cacccgatac tgcaggccac gcaccagatg
    26221 gacgtcgaac tggtcggcgt agccgtcgaa gatgccggtc accacctggg ctggctgcgt
    26281 cggcggcgtt tgcccatgaa ccacggcgtg ccagtagcgc gcgttctcgt cgaagggttt
    26341 gagggccagc agacgctcgg cgtaagtccg ggcccgcgcc tgatcgtcca tgctcagggc
    26401 gcagctgagc aggccctgca aggtctcctc gtggtgcggc aggcgctgga ggatgaactc
    26461 gtagtgggtg gtggcgtcgg ccaaccgccc ctgcgccgcc agaaagcgcg ccagaaacag
    26521 gcggatgccg gcgctttcgg ggaagcgccg cacgccgtcc tcggcccact tcagtgtctg
    26581 ctcatggcgt tccgccgaca gggccacgtt gatggcaccc accatcacct gagcctcctg
    26641 gggggcgctg gccagcgccc gcatggccag ggagatggcc tcgtcgccct gcttctgcct
    26701 ggccagcagc agcgccagct cgatctgtgc cacatgccag tccggcgcca gcgccagcgc
    26761 gcgacgggcc gcctgggtgg cgcgctccag gttgccggcg cgttcggcca gccgcatgcc
    26821 cagcagcggg atgcgggcgt ccttgggcgc ctgctgctgg gccgcgttga gcgcctgcgc
    26881 ggcctgtggc agttgatggg cttcgatcag gccgcggatc tgctgcagct ggagggtgaa
    26941 ggggtcggcg gtgggggtgg aagcaagcat cggtggacct gggggaggaa ccgtcagagc
    27001 cgcgcgagcg cgcggtcggt agccctgatg tcggttcccg gattatcctc aatgcgccag
    27061 ttgaccgctg gtgcccctgg tgagatgtgc atcccaacgg ggtctcgcgg catcgatggg
    27121 tttgacctgt tcggtgagcg cgctacgcac ctgtccgtac cgcatcggcg cgcggcacga
    27181 gcgggcgcgt ccaatggccg ttgccaggct gcaacgaaga tcgccggcgc ttgtccgtca
    27241 agcgcgacga tgctgcggtc agcgggcagg ctcgccatcg cggcgcgtca gccacatggc
    27301 gtcgccatag ctgaagaagc ggtagcgtgc cgccaccgcg tgggcgtaca gcgccatcac
    27361 atggtcgtgt cccgccaggg cgctgaccag catcatcagc gtgctgcgcg gcaggtggaa
    27421 gttggtgacc agggcatcga ccacacggaa gccgaagccc ggggtgatga agatgtcggt
    27481 gtcgccctgc cgctggccgc tggcggccca gctctccagc gtgcgcacgc tggtggtgcc
    27541 taccgccacc acgcgaccgc cgcgcgcgcg gcactcggcc accgcctgcg cggtggcggg
    27601 cggcaccagg tagcgctcgc ggtgcatcag gtgttcggcc aggttctccg tcctgaccgg
    27661 ttgaaaggtg ccggcgccca catgcagggt gacgaaggcg cgctgcacgc cacgcgcgtc
    27721 gatggcggcc agcagcgcgt cgtcgaagtg cagcgccgcc gtcggcgccg ccaccgcgcc
    27781 ggggtggcgc gcgaacacgg tctggtagcg cgccacgtcc tgcgccgagt cggtgtgctg
    27841 gatgtagggc ggcagcggca catgcccgtg cgccgccatc agctggtagg catcgcccga
    27901 cagacgcagg tgaaacagcg gcccgtcggc ctgcggccag cggcccagca cggtggccgt
    27961 gaagtcgccg gccatgcgca gcacggtgcc gggcgggggc ttcttgctga ccttcatgtg
    28021 cgcggccacg ctgccgtcgg gcagcacccg ctcgaccagc agctccagct tgccgccact
    28081 gggttgccgg tcggcgccgg gccgccccaa gccgatgggc gccccttgag gggaacggcg
    28141 cgaacgcgcc aacggggcgg gtggtttgtg gccgtacagc cgggccttga ccacctgggt
    28201 gtcgttgaac accagcaggt cgcccggctg cagcagcgcc ggcaggtcgc gaaacacccg
    28261 gtcgatgggt gcggcgccgc tgccatccag caggcgcgag gcgctgcgct cgggcgccgg
    28321 gtgctgggcg atcagctcgg gcggcagggc aaagtcgaaa tcgctggtgg tgaaggtgcg
    28381 gtcagacatg cttgagggcc ggacgggccc gtaccaagaa gaagggaagg cagaattgtc
    28441 ccatgcccgc caccgaggcc accgccgccc ccgccgccga gcgcgcgctg tccgcgcccc
    28501 agaaggcgct gcgcaagctc ggcctggtgc ggccgatcga tctggcgctg cacctgccgc
    28561 tgcgctacga ggacgaaacc cacctgacgc cgctggccga ggcggcgcgc accctgagcg
    28621 acgcgccgct gcagtttgaa gcgaccgtgg tgcatcacga agtggcgtat cgcccgcgcc
    28681 gccagctgct ggtgacggtg gaagacggtg gcgagcgctg cctgctgcgc ttcttcaatt
    28741 tctatccctc gcagctcaag cagatggcgg tgggcacgcg cctgcgtgtg cgcggcgagc
    28801 tgcgcggtgg cttcggtggc ctcgccatga tgcatccggt ggtgcgcgcc gccggcggcg
    28861 cgctgccgca ggcgctgacg ccgatttacc ccacggtggc cgggctgccg cagccctacc
    28921 tgcgcaaggc ggtgctgggg gggctggagc aggccgatct gagcgacacg ctgcccgacg
    28981 aggttttgag cgaaatcggc catctgcgcc cgtgggacct gcgcaaggcg ctatcttttt
    29041 tgcaccaacc gacgcccgat acggcgctcg cccagctgga agaccacagc caccccgcct
    29101 ggcagcgcct gaaggccgag gagctgctgg cgcagcaact gtcgcagctg cgggcgcggc
    29161 gcgcgcgcca gcacctgcgc gcgcccgtgc tgcctgccca gcgcggtggc ctgcacgagg
    29221 cgctgctggc ggcgctgccg ttccagctca ccggcgcgca gcgccgcgtg ggcgaggaga
    29281 tcgcacgcga tctggcgcgc ccgcagccca tgcaccgcct gctgcagggc gatgtgggca
    29341 gcggcaagac ggcggtggcg gcgctggccg cctgcatcgc catcgacgcc ggctggcagt
    29401 gcgccctgat ggcgcccacc gaaatcctgg ccgagcagca tttcaccaag ctggccgact
    29461 ggctggcgcc gctgctggcg ccgcgcggcc ggcaggtggc ctggctgacc ggcgggcaga
    29521 agaagaaggc acgcgccgac atgctggccg ccatcgaatc cggcgcggcg gcgctggtga
    29581 tcggcaccca cgccgtcatc cagcagcagg tgcgctttgc ccggctgggc ctggcggtca
    29641 tcgacgagca gcatcgtttc ggcgtgcagc aaaggctgga cttgcgcaac aagctatcgg
    29701 aagcagagca ggagccacac ctgctgatga tgagcgccac ccccatcccg cgcaccctgg
    29761 cgatgagcta ctacgccgac ctggacgtct ccaccctcga cgagctgccg cccggccgca
    29821 cgccggtcat cacccggctg gtggcccagg cgcgccgcgg cgaagtgatc gagcgcatcc
    29881 gtgcccagat cgctcagggt cggcaggtct actgggtgtg cccgctgatc gaagagagcg
    29941 aagcgctgga cctgcgccac gccagcgcca cccacgccga gctggcggcc gcgctgccgg
    30001 ggcaggcggt gggcctgctg cactcgcgcc tgccgccggc cgacaagcag gcggtgatgc
    30061 agcaattcgt cagcggcgcc atgccggtgc tggtgtccac caccgtgatc gaggtcgggg
    30121 tcgacgtgcc caacgccagc ctgatggtga tcgagcacgc cgagcgcttt ggcctgagcc
    30181 agctgcacca gttgcgcggg cgcgtcggac gcggcgccgc cgcttcggcc tgcgtgctgc
    30241 tgtatgccac gcccgaaggc ggccggctgg gcgacagcgc gcgcgagcgc ctcaaggcca
    30301 tggtcgaaac cagcgacggt ttcgaaatcg cccggcgcga tctggccatc cgcggcccgg
    30361 gcgaattcct gggcgcacgc cagtcgggcg acgccctgct gcgctttgcc gacctggccg
    30421 aagacgcacc gctgctggcc tgggcccgcc gcgccgcgcc gcgcctgctc gaccactggc
    30481 ccgaacgggc cgcgcggcat gtggaccgct ggctgggcgg gcgggccgaa tacctcaagg
    30541 cctgacggtg gcgcggggtg aaactgagcc gcactgacct ggtacacgcc gtgccctgcg
    30601 ccggccgccc ggcctgcggc acgctctggc gctatcgtgt cggggcggcg cgccggccgc
    30661 cggcccgtcg cggcgctccg gccgtgcggg tcgccaaccc ttgcctggag ctgccatgac
    30721 cctgaccgaa ctcaaataca tcgtcgccgt ggcgcgcgaa aagcacttcg gcaaggcggc
    30781 cgaggcctgc ttcgtgtcgc agcccacgct gtcggtggcc gtcaagaagc tggaagagga
    30841 gctggaggtc aaactgttcg agcgcggcgg cagcgagatc gccgtcaccc cgctgggcgc
    30901 cgaaatcgtg cgtcaggccc agagcgtgct ggagcaggcc gccgagatca aggaaatcgc
    30961 caagcgcggc aaggacccgc tgggcgggcc gctgacgctc ggcgtgatct acaccatcgg
    31021 cccctacctg ctgcccgagc tggtgcgcca caacattgag cgcacgccgc agatgccgct
    31081 gatgctgcag gaaaacttca ccgtgcgcct gctggaaatg ctgcgcaccg gcgagatcga
    31141 ctgcgccatc ctggccgaac cctttcccga ctcggggctg gcggtggcgc cgctgtacga
    31201 cgagcccttc atggccgcgc tgccggccgc tcacccgctg gcgcgcggcg ccgccgtcac
    31261 gtcggagcag ctgcgcggcg aacacatgct gctgctgggc accggtcact gctttcgcga
    31321 ccatgtgctg caggtctgtc ccgagttcgc cggcttcgcc agcagtggcg aaggcatccg
    31381 caggaacttc gaaggctcgt cgcttgaaac catcaagcac atggtggccg ccggcatggg
    31441 cgtgaccctg gtgccgcggc tggcggtgcc ggtcgaggcg ctggtggccc aggccgcgca
    31501 ggccgacgcg cccggggcgg tgcgctacct gccggtgcgc gacggcggcg gcgccgcacc
    31561 gccgacgcgc cgcgtggtgc tggcctggcg ccgcagcttc acccgctacg aagccatcgc
    31621 cgccctgcgc aacgccatct acgcctgtga gctgccgggg gtgcagcggc tgtcgtgagt
    31681 cgaaaccttg gttaaactgg gctgcgtggc gctttcagca agcgccgaca gctatcgatt
    31741 cagtagtatg acaggctccc catcgtcccc gccccgcagc aagtggtcgc tctatctcga
    31801 cctgatccgc tgggatcgcc cggccggctg gctggtgctg ctgtggccca ccctgtcggc
    31861 gctgtggctg gcggccggcg gctttccggg ctggcacctg ctggcggtgt tcgtcgccgg
    31921 caccatcttg atgcgctcgg ccggctgctg cgtcaacgac gtggccgacc gcgagttcga
    31981 ccgccacgtc aagcgcaccg cgcagcgccc ggtcaccacc ggcgcggtcg gcgtgcgcga
    32041 ggcactggcg ctgggcgcgg tgctggcgct ggcggccttt gtgctggtgc tgaccaccaa
    32101 cgccagcgcc attgcgctgt cgtttccggc gctggccatc gccatcgcct acccgtttgc
    32161 caagcgctgg gtgtcgatgc cgcaggcggt gctgggcgtg gccttcagct tcggcatccc
    32221 gatggccttt gcggcggtgc agggccaggt gccgtggcag gcctgggcgc tgctggcggg
    32281 caatctgttc tgggtgctgg cctacgacac cgaatacgcc atggtcgacc gcgacgacga
    32341 cctgaaaatc ggcatccaga cctcggccat caccctgggc cgctgggacg tggcggcgat
    32401 catggggttt tacgccgctt acctgtcgat ctggattgtg atcgttttgc agcaagcgcc
    32461 gacagctatt gtttacatag cgttcgggct ggccgcgggg caggcgctgt ggcactacac
    32521 gctgatccgc gggcgcaccc gtgaaggctg cttcacggcc ttccggcaga accactggct
    32581 gggcgccacg gtgttcgccg gcatcgcgct ggcgctgcga tgagggtggt ttcagcgcag
    32641 cgcggcgtac tggttggtca catcgtccag caccagcagc aggttctcgc tggtggtcat
    32701 gatgtgcgcc aatgcttcct tgaccggctg gccgctgcgg ctggacgacc tgatggcggc
    32761 atcgaacaac agccactgct gctcgcccag tttcagcgtc tcgcggatgc gcggcgtggc
    32821 ctggggcgcc tcggccagga cctcgaggcc ggtcacgaac tcgctgcggt tcttttcgat
    32881 ctccgtggtg gcggttgcca gctggatgcg cgccccgctg gccagcgcgt acttggccag
    32941 ccgctgggac agcatgcgct ggcgcccggc caggttgacc aggcgccctg acggctggcc
    33001 ggatgccttc tccagttgcg tggtgccctg gtgtgccagc gccagcacgg tttcggactg
    33061 tgccagcacg gcgtcggcgc ccgccatcga ggcgggcttg ccgaccagcc tgtccttgaa
    33121 atccagccag gcgcgttcga gttggccgta ggtgtcgcgg atgcccgggt tgggggcgaa
    33181 acccttgagt tcgaccagtt ggcggtcaaa cagcgccatc gactggctca gcaccttttc
    33241 cgcctgcgcg ggcagcgcct ccagcgccac acccagatag gccttggcca cgcgctgcga
    33301 cagcatgcgc tggcgaccgg ccttgttgat ggcgtcgttc atgtccttga cctgggccca
    33361 aacgggcggg atggtcaggc cgaccaaccc cagggcggcg aactgacggc gggaaagcgt
    33421 tggcatgagc ggcactccat ttcctgcggc ggggtcaggg tgtgtccttc cagtggccgc
    33481 gcacccgcgt caacccggtc aacacctgcc actcgcagga tcagagcgaa atagtaatat
    33541 caaagtacac gacgcaacct tgacctggct tcaggtttcg ggtgcatggc gcgccggcag
    33601 cagggtaggc cccggccgac ccggttgaaa ggcaatgcgc tcagcgcccg aattcgtcgc
    33661 ccagttcctt ggcgcgcgca caggcggcgt gcatggcggc gacgaacgcg ggcttgacgc
    33721 cggcctggtc cagcgcggtc agggcggcgt aggtggtgcc gcccttgctg gtcacgcgct
    33781 cgcgcagggt ggccagcggc tcgctcgagg cggccgccag gtgcgcgccg ccggcaaagg
    33841 tggccacggc caggcggcgc gcctgttcgg gcgacaggcc cagctcgctg cccgcctgct
    33901 gcatggcttc gaggaaatag aacacatagg ccgggccgga gccggacagg gcggtgacgg
    33961 cgtcgagctg cgcctcgtgc tccagccaca gcgcctcgcc ggtggtgcgg atcacggctt
    34021 cggccagcgc gcggtcggcc gcgctcacct cgggcgcggc aaacagcccc gtcatgccgc
    34081 gcccgaccag ggccggggtg ttgggcatgc agcgcacgat gcgccgcgcg ccgctggcgg
    34141 cggcgatgtc gccgctgcgg atgccggcca tcaccgacag ctgcagcgcg ctgcccacgt
    34201 gtggtgccac cggcgccgcg gcggcaccga agctctgcgg tttgacggcc cagacgatca
    34261 ggtcggcgcc ggcgagctgg gggccggcgg cgggcagtgc ggtcacgccg tgctgctgca
    34321 acagggcggc gcgcgtg
//
LOCUS       DAOQTZ010000014        33415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1523_length_33415_cov_13.621133, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000014 DAOQTZ010000000
VERSION     DAOQTZ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 33415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33415
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_33415_cov_13.621133"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..450
                     /locus_tag="PKN51_03120"
     CDS             49..450
                     /locus_tag="PKN51_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84120.1"
                     /translation="MFFHSLPPPAPVSGGADGLLARVLGALVWLMLAALGAVFALSLL
                     IWLVVMVVVSLVGSLFTGRPATVTLLWRRYRDMTREVTRSRWSPSPVSSTPRADAAQA
                     TGAASAQTSVQDVSWREVPAAPAADGDAPGR"
     gene            complement(573..2135)
                     /gene="lysS"
                     /locus_tag="PKN51_03125"
     CDS             complement(573..2135)
                     /gene="lysS"
                     /locus_tag="PKN51_03125"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNT84121.1"
                     /translation="MNQPAAPADLQAAASEHDENKLIAERRDKLRAIRAVQAAGGAVA
                     FPNDFKPKDRASQLQQAHGAIEKEVFEADLAAGRQRHASVAGRMMLKRVMGKASFATL
                     QDATGRIQIYVAREAVGEADYEAFKHWDLGDIVACEGHLFKTRTGELSIHATRIRLLT
                     KSLRPLPDKFHGVQDQELKYRQRYVDLIMDGEARTRFAARSATLSAMRAFMTGHGFME
                     VETPMLHPIPGGANAKPFVTHHNALDQQMFLRIAPELYLKRLIVGGFERVFEINRSFR
                     NEGISVRHNPEFTMMEFYAAYWNYQDLMDFTEALLRDTAQQATGSLQLSYQGRAVDLS
                     QPFQRMTIRQALHTHTDIGAHVDDAAELIRRLKKLGLSEDKDRLSQRSLASLQVMYFE
                     EEVEQHLWQPTFIMEHPTEISPLARANDQRPEVTERFELYITGREMANGFSELNDAED
                     QAARFAAQASAKDAGDEEAMYFDQDFIRALEYGMPPTGGCGVGIDRFMMLLTDSQSIR
                     DVILFPALRHEA"
     gene            2337..3641
                     /locus_tag="PKN51_03130"
     CDS             2337..3641
                     /locus_tag="PKN51_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNT84122.1"
                     /translation="MKAASGSQPSLTRATLTLLGGGALAQLLPLLLGPFIARLFTPEA
                     FGLYTRFATVAAALAVAASLRYEQALPLARSDDEARALLALALRLLLAAVLLGVPLGW
                     ALHALGALPLAVLLAPALATAGLLQVLMLWANRAQRFGALATSRVLHYGGAAALQLVL
                     GWALWRGAASGAEGAWALALAPLLAQLLACGGLWRPAPAGGWRALLVPASAALRTQMR
                     TVARQQRDFALINTPHALLGLLQDALAVALLVAWSGEAAAGFWGLALRYLKAPATLVG
                     GAVSQALYPRLAAARADDARRLVRRLMALLGALALGLMALLLLAGPWLFERVFGPPWR
                     EAGELARALAPYIAAHFVAAPLAVVTMAWRAQRWAFRLALLGQLAFLAALALGLRQGG
                     LLGGAWAVSAVMLPYFGWYFWRLAGWRDIPSPLGVPPREGAP"
     gene            3647..4222
                     /locus_tag="PKN51_03135"
     CDS             3647..4222
                     /locus_tag="PKN51_03135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNT84123.1"
                     /translation="MRAALNRLRRRLWRLLFHRMLFGEASAGVLRPATRLSPTVCIEH
                     EPTLTLADDVFIGHFCFIEASGGVRIGGGTQVTNFVSIVSHSTHRALRVAAGLSGAPL
                     EAAVRRAPIVIGERCFIGPHSTIEAGSTLGHGTLVAAHSRVRGVFPDFAVLAGSPAQV
                     VGDTRAADERWLAAHPELLAAYRRWRQGLAQ"
     gene            4219..5277
                     /locus_tag="PKN51_03140"
     CDS             4219..5277
                     /locus_tag="PKN51_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT84124.1"
                     /translation="MTAPHLGLLGDASSVHVQRWAREMRARGWRVSLVTARPAPIEGV
                     SQIVLRPVRRASDWLWRVGEARRALTQLQPDLVHAHYVTSYGYLAARAGLQPLVLTAW
                     GSDLLVTPRQSALKRWLTAWSLRRARLVTGDSRDLLAAARALAPGVPTQLVHWGVERS
                     RFAPVPWADKPAAEAVSPRNWEPNYRIDVVLRALAQVRAVRPEARLHLLGGGPLEADL
                     RALTGQLGLQDAVRLHGRLDDAGMAAVLARCKLSISVPESDATSVSVLESMACGLAVI
                     ASDLPANRDWLAPEALVAAGDVAALAARWLALLQDDGRAAVLGAANAERIARDGDRRV
                     QMDAIDAACRRLLQEAGR"
     gene            5274..6338
                     /locus_tag="PKN51_03145"
     CDS             5274..6338
                     /locus_tag="PKN51_03145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT84125.1"
                     /translation="MTQELATPPLPQLLTPGEAGLVSVVAPCRNERAHIDAFCDSALA
                     QRLPEGWRMELLVADGASDDGTRARLAERAAADARLRLLDNPRRIVSSGLNACLAQAR
                     GQVIVRMDIHTRFAPDYVAECIAALARSGADNVGGPWVARGQGGWGRAIAAAFQSRWV
                     VGGARSRDAAFEGEVDTVYLGCWPRASLARVGGFDETLVRNQDDEHNLRLRLAGGRIW
                     QSRRIHSSYRPRDSLRQLFEQQLQYGYWRPFVLRKHRQAGSVRQLVPMVFVAAGAFCA
                     LLAPVFGLGLKAWAAAYAAYLLAASAQAAHQAGEARLAWRLPWVIAAYHLGYGLGSWR
                     GVWGLWRRRAAPQAATRLTR"
     gene            6379..7539
                     /locus_tag="PKN51_03150"
     CDS             6379..7539
                     /locus_tag="PKN51_03150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT84126.1"
                     /translation="MSVSESFLPFARPDIGEAEIDAVARALRSGWVTTGPETRAFEQE
                     FAAFLGGGVQAIAVNSATAGLHLALEAIGIGPGDEVIAPTLTFTATVEVARYLGADAV
                     LVDVDPVTLNIDPAAIEAAITPRTRAILPVHYGGLACDMAAIHAIARRHGLQVVEDAA
                     HALPASCGGTPVGQLPSAATVFSFYANKTMTTGEGGMVVTRDEALAARMRVMRLHGIS
                     RDAFDRFTSRTPAWYYEVVAPGFKYNLTDLAAALGRVQLARLPAFVQRRQQLAARYLR
                     ELAGLPLVLPPQAPAGDTHAWHLFVLRLADTARVTRDEVVQALADAGIGTSVHFVPLH
                     RQPYWRDRYALRPQDFPVAEAAYQRMLSVPLFTAMSDAEQGRVIAALRACLQ"
     gene            7536..8243
                     /locus_tag="PKN51_03155"
     CDS             7536..8243
                     /locus_tag="PKN51_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84127.1"
                     /translation="MTPARLAASPWPWFGWSARAWARPWRAFVRDFAGWQGECGLELG
                     AGPRSALAPLMLSLVRQVECSAQDAASLPVIEARHRDLLAPQDAARIAHGVRDLRALH
                     GQWDLIVMKSVLGGVHRLGRSSLADVHTTLEQLVARHLRPGGLLVTLDNGRTALEPLW
                     SRFGARRNGWRFFTRDEFPPAAAAYGFGLLGSFSAATRLGRPGRWSDDALYGADLLLS
                     PWVRRHAVLLHVYRKPA"
     gene            8240..8869
                     /locus_tag="PKN51_03160"
     CDS             8240..8869
                     /locus_tag="PKN51_03160"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNT84128.1"
                     /translation="MSTTSTDAAKRVFDLALALPALLLALPLMAAIALWVRLDSPGPA
                     WFRQARVGRGGRLFRIHKFRTMQVQAVAGGPLITVAGDARVTRAGRWLRRTKLDELPQ
                     LLDVVRGDMSLVGPRPEVPRYMALYPDEARRLILSVRPGLTDRASIEFRDEERLLAAA
                     SDPERAYVEQVMPIKQRYYLDYVAQRSLTGDVGILWHTLRAVLRRPDSR"
     gene            complement(8934..9578)
                     /locus_tag="PKN51_03165"
     CDS             complement(8934..9578)
                     /locus_tag="PKN51_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNT84129.1"
                     /translation="MADAPLTLTSLPLFPLDTVLFPGGWLPLRVFEVRYLDMIGRCQR
                     AGAPFGVVALAQGQEVRTPGAPQERLQPVGTLAHVRTLTQPQPGLMLIECQGEQRFRI
                     DQPHQLRHGLWVADVSLLPPDPVVTIPDDLQAAARTLRRAHDTLRQQPQGLRLPGGDA
                     LDYDDCGWVANRWCELLPLPTATRQHLMSLDSPLLRLELVVDLLSRTGIGGAPR"
     gene            9702..10271
                     /locus_tag="PKN51_03170"
     CDS             9702..10271
                     /locus_tag="PKN51_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNT84130.1"
                     /translation="MLYTILSFLLEIGVTLVGGACLLRLYMRWRRMSFDNPVGQFVLA
                     LSDWLVQPLQRVVPPAGGLDVASLLAAWLLKLVQYALLMALLELPRWGLLPVLALLGT
                     ARLAVSVSTALVIIAAVLSWTGQRSRAHDVFERLCAPLLAPIRRRIRLVGGVDLSPLL
                     LIVLLQVLGIVLSSLQSGLLGPGVPALAG"
     gene            complement(10441..11313)
                     /gene="accD"
                     /locus_tag="PKN51_03175"
     CDS             complement(10441..11313)
                     /gene="accD"
                     /locus_tag="PKN51_03175"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNT84131.1"
                     /translation="MSWLEKLLPPKIQKSDAAERRQMPEGLWIKCPDCDTVLYKTDLE
                     QNQNVCPHCSHHHRIGARERLNAFLDGEGRYEIGQEVVPVDALKFKDSRKYPERLKEA
                     LENTGETDALVVMGGAVRSISVVAAAFEFDFMGGSMGSVVGERFVRGVQTAIEQKVPF
                     ICFTATGGARMQEGLFSLMQMAKTNAALTQLAKKGLPYISVLTDPTMGGVSAGFAFVG
                     DVVIAEPKALIGFAGPRVIENTVRVTLPEGFQRAEFLQQKGAVDFICDRRELRQKVAS
                     ILAMLQRQPADAVE"
     gene            complement(11341..12150)
                     /gene="trpA"
                     /locus_tag="PKN51_03180"
     CDS             complement(11341..12150)
                     /gene="trpA"
                     /locus_tag="PKN51_03180"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNT84132.1"
                     /translation="MSRIAATFEALQAQGRKALIPYVTAGFPYADITPAVMHAMVAAG
                     ADVIELGVPFSDPSADGAVIQKAGERALALGIGLGQVLAMVRSFRAKDGDTPVVLMGY
                     ANPVERYDQKHGDGAFIRDAAAAGVDGVLIVDYPPEECRDFAARLRAAGLDLIFLLAP
                     TSTEARMRQVAEVASGYVYYVSLRGVTGSGALDTAEVGAMLPRIRQHVHIPVGVGFGI
                     RDAETARAIGQVADAVVIGSRLIQQIENQPHERVAAAAADFLRPMRAALDA"
     gene            complement(12147..13412)
                     /gene="trpB"
                     /locus_tag="PKN51_03185"
     CDS             complement(12147..13412)
                     /gene="trpB"
                     /locus_tag="PKN51_03185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNT84133.1"
                     /translation="MSDYHQPDASGHFGRYGGSFISETLTYAINELRDAYARYQHDPE
                     FQREFRHELAHYVGRPSPIYRAARTSRELGGAQIYLKREDLNHTGAHKINNVIGQAML
                     AKRMGKPRIIAETGAGQHGVATATICARYGLQCVVYMGSEDVKRQSPNVYRMKLLGAT
                     VVPVESGSRTLKDALNEAMRDWVANVDHTFYIIGTVAGPHPYPMMVRDFQSVIGQECL
                     TQMPELLAEQHMAARQPDAVLACVGGGSNAMGIFHPYIPFENTRLIGVEAAGQGLDSG
                     KHSASLQRGSPGVLHGNRTYILQDDNGQITETHSISAGLDYPGVGPEHAWLKDTGRAE
                     YVGATDAEALAAFHHLCRTEGIIPALESSHAFAHAMKLAPTMRPDQSILVCLSGRGDK
                     DIGTVADLDGADFYDRPSMRGHVVKGARA"
     gene            complement(13409..14140)
                     /locus_tag="PKN51_03190"
     CDS             complement(13409..14140)
                     /locus_tag="PKN51_03190"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNT84134.1"
                     /translation="MTPVSLALAGARTRIKICGLTRAQDVDAAVAAGVDAIGFVRYPR
                     SPRHVDIRRAAELARRLPPMVTPVLLYVNADASEIIADCALLTGATLQFHGDESAADC
                     ARVAARCGRPYWRAARIPAGAAGAFDLIEFAHAFATAQALLLDAHVEGFGGGGKTFDW
                     SLLPPNVAAHLVLSGGLTPANVGDGLRLLRGRGRSLAVDVSSGVEAVGPDGQPLKGIK
                     DAARIFQFVAAVRAADAALAGSPPT"
     gene            complement(14137..14922)
                     /gene="truA"
                     /locus_tag="PKN51_03195"
     CDS             complement(14137..14922)
                     /gene="truA"
                     /locus_tag="PKN51_03195"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNT84135.1"
                     /translation="MRIALGLSYLGTPYQGWQSQPTGQTVQDRLEAALTAFATSPIAT
                     LCAGRTDAGVHGLMQVVHFDTELERDAFSWVRGTNRFLPRDIAVQWAQPVPQEFHARA
                     SALARRYTYVVLEAPVRPSLESGRVGWAFRPLDGVAMRRAADRLIGEHDFSSFRASAC
                     QALTPVKQLQRIDIRRCGAYWRFDFEANAFLHHMVRNLMGCLVAIGQGLHPPEWIDSV
                     LAARSRDAAAPTFAPDGLYFLGPRYDARWGLPERTPAYDWLPS"
     gene            complement(15035..17758)
                     /locus_tag="PKN51_03200"
     CDS             complement(15035..17758)
                     /locus_tag="PKN51_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNT84136.1"
                     /translation="MQNKTAMTLLGSIASSHSVRQPWRKGVLASAVAIATCGVSLDAQ
                     ALALGAITVRSALGEPLRAEIEVPQISSEEAATLKANIGSPEAFRAAGVDFPAALAGA
                     RVSLQRRASGQAYLRVTGERTVNEPFLSIVVEANWANGRVVRDYTLLVDPPGRGAPPP
                     VSVTASQATAPPTSAVVHAPRAAPLPPVAAADAGSRPRRDIHPAEAPRQAVSTTGEGA
                     NVTVQRGDTAGSIARAHAIEGVSLDQMLVAMLRTNPDAFINGNVNLLRAGTVLQMPSV
                     EQASAVTRQAARRAIVAQTRDFNAYRLGLAQRSGATRVATAATGSASGGVQPEVRDSR
                     AAAPSPDRLKLSREGAQTGAESQLAQSRQAQEQSDRVAELNKNLSELARLQAASGSAT
                     STGVKLPIGTPPAAAPVAATPAAPAASVAAEPAAPTVATPASASEPASVASAPAPAPA
                     PTMADAASAVPAAPASAAMAEPAAVPASGVSETVTAPVAAPAPQAAPVPPTTASEGPS
                     FLDALTDNPLLPIAGAGLIALLAGFGVYRWRQSKKGDATMDSSFIESRLQPDSFFGAS
                     GGQRVDTREGRPSVGSSSSMVYSPSQLDAAGDVDPVAEADVYLAYGRDMQAEEILKEA
                     LRTHPGRVSVHRKLAEIYARRRDARALEMVASEAWNHVKGEGADWAAIASLGSELDPD
                     NPLYKPGGVPMMRSAATATTPQPRFGADTEPQTAQLQDKRDSKPDSSSIPLDLELDLD
                     DAPVRASAVTSVPPSVGNTSAMPSHHGPEPAIAAAPEVKPVPIALPDFDLDLDTPTTV
                     APALAAAVATQADDEPPSVAQPYVASKAGMIDFDMEALSIDPDSRSGVDPKTEQPDDA
                     DDDPVATKLALAQEFHAIGDTEGARTLVREVIAEATGSLRMRAERFLAELD"
     gene            complement(17959..19095)
                     /gene="asd"
                     /locus_tag="PKN51_03205"
     CDS             complement(17959..19095)
                     /gene="asd"
                     /locus_tag="PKN51_03205"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNT84137.1"
                     /translation="MKTGNVVGLVGWRGMVGSVLMDRMQAERDFDLIEPIFFSTSNAG
                     GAAPAQARNERQLKDAHDIEALKKCDIILTCQGGDYTHAVYPKLRAAGWGGHWIDAAS
                     ALRMERNAVIVLDPVNLPVIQNALAKGGRDWIGGNCTVSCMLMGVGALYKAGLVEWMS
                     TQTYQAASGGGAQHMRELLAQFGTLNHEVRDLLSDPTSAILEIDRKVIGKQRALDDAE
                     KANFGVPLGGSLIPWIDKDLGNGMSREEWKGMAETNKILGQGEGFDTPAVPVDGYCVR
                     IGAMRCHSQALTFKLRKDVPVADIEAMIAADNDWVKVVPNNRDATLRDLTPVAVTGTL
                     TIPVGRIRKMAMGPQYLGAFTIGDQLLWGAAEPLRRMLRILLQD"
     gene            complement(19562..20632)
                     /gene="leuB"
                     /locus_tag="PKN51_03210"
     CDS             complement(19562..20632)
                     /gene="leuB"
                     /locus_tag="PKN51_03210"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNT84138.1"
                     /translation="MKIAVLPGDGIGPEITSEAVKVLQALGLKLELESAPVGGAAYEA
                     AGHPLPESTLNLARAADAILFGAVGDWKYDTLDRPLRPEQAILGLRKHLGLFANFRPA
                     ICYEQLTAASSLKPELVAGLDILIIRELTGDIYFGQPRGRRQAPDGHFPGAEEAFDTM
                     RYTRPEVERIARVAFEAARQRSRRVTSVDKANVLETFQLWKDVVTEVHKDYPDVELSH
                     MYVDNAAMQLVKAPKAFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLNDKKQGLYE
                     PSHGSAPDIAGKGVANPLATILSAAMMLRFSLGQPEAADRIEAAVRKVLAQGLRTPDI
                     WSEGTTRVGTREMGEAVVRALD"
     gene            complement(20734..21390)
                     /gene="leuD"
                     /locus_tag="PKN51_03215"
     CDS             complement(20734..21390)
                     /gene="leuD"
                     /locus_tag="PKN51_03215"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNT84139.1"
                     /translation="MDAFTTHKGLVAPLDRENVDTDAIIPKQFLKSIRKTGFGPNLFD
                     EWRYLDHGEPGMDPASRKPNPDFVLNQPRYQGASILLARQNFGCGSSREHAPWALQQY
                     GFRAILAPSFADIFFNNCFKNGLLPIVLPESAVDRLFHDVAAFPGYQLTIDLQRQVVV
                     KGDGTELAFDIDPFRKHCLLGGLDDIGLTLQHKDAIARFEAERLASKPWLAHTMGRPA
                     "
     gene            complement(21581..21703)
                     /locus_tag="PKN51_03220"
     CDS             complement(21581..21703)
                     /locus_tag="PKN51_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HNT84140.1"
                     /translation="MKKVLALVAAALMLAGCNTVQGVGKDVQKAGSAVENAGKK"
     gene            complement(21723..23177)
                     /gene="leuC"
                     /locus_tag="PKN51_03225"
     CDS             complement(21723..23177)
                     /gene="leuC"
                     /locus_tag="PKN51_03225"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNT84141.1"
                     /translation="MTPRTLYDKIWDEHVVRTEEDGTALLYIDRHLVHEVTSPQAFEG
                     LRQAGRKVWRTSSVVATADHNTPTTGWERGYDGIEDPISKEQIVTLDANMQAHGAAAY
                     FPFMNQRQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
                     QTLIAKKARNMLVRVNGAVAPGITAKDIVLAIIGRIGTAGGTGHTIEFAGQAIRDLSM
                     EGRMTVCNMAIEAGARAGLIAVDDKTIDYVKGRPMAPGTDPVTGRFVGGPEWDQAVAS
                     WRSLRSDDGAHFDTVVELDAQAIVPQVTWGTNPEMVVGVDARVPDPDKEKDPVQRGAM
                     ERALAYMGLIPGKPIADIHVDKVFIGSCTNSRIEDLRAAAVVVKRLGRRLAPNVKLAM
                     VVPGSGLVKQQAEAEGLDKIFTAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNF
                     EGRQGQGGRTHLVSPAMAAAAAVHGHFVDIRQIS"
     gene            complement(23453..24748)
                     /locus_tag="PKN51_03230"
     CDS             complement(23453..24748)
                     /locus_tag="PKN51_03230"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNT84142.1"
                     /translation="MKLSDNKAKLSFSNGSPDIELPVYQGSIGPDVIDIRKLYGQTGM
                     FTYDPGFLSTASCQSAITYIDGDKGELLYRGYPIEQLAQKCDYLDTCYLLLNGELPNP
                     TQRSDFHKLVLKHTMVNEQMQFFLRGFRRDAHPMAVLTGLIGALSAFYHDSTDIHNPE
                     HRHISAIRLISKLPTLVAMAYKYGVGQPYMYPRNELSYAGNFLRMMFGTPCEDYKVNP
                     VLEKAMDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAC
                     LNMLEDIQRNGGIAKVGEFMEQVKDKNSGVKLMGFGHRVYKNYDPRAKLMQETCNEVL
                     AELGLEKDPLFALAKQVEKIALEDDYFVQRKLYPNVDFYSGIVQRAIGIPVSLFTGIF
                     ALARTVGWIAQLNEMLADPEYKIGRPRQLFTGSPRRDVP"
     gene            complement(24799..25071)
                     /locus_tag="PKN51_03235"
     CDS             complement(24799..25071)
                     /locus_tag="PKN51_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HNT84143.1"
                     /translation="MESERIDEKALGMLRWRCRRGMLENDLLIERFFQRHAEQLTASQ
                     AAALTQLMDLSDNELLDLLLRRKEPDGDLDTPDIRQLLTLLRPAAA"
     gene            complement(25098..25802)
                     /locus_tag="PKN51_03240"
     CDS             complement(25098..25802)
                     /locus_tag="PKN51_03240"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNT84144.1"
                     /translation="MQRTFKIYRYDPDKDAKPYMQTVQVELDGHERMLLDALLKLKAQ
                     DPSLSFRRSCREGVCGSDAMNINGKNGLACLTNMNTLKGDIVLKPLPGLPVIRDLIVD
                     MTQFFNQYHSIKPYLQNDQPTSPSKERLQSPEEREELNGLYECILCASCSTSCPSFWW
                     NPDKFVGPAGLLQAYRFIADSRDQATGERLDNLDDPYRLFRCHTIMNCVDVCPKGLKP
                     AAAISKIKELLVRRGI"
     gene            complement(25835..27640)
                     /gene="sdhA"
                     /locus_tag="PKN51_03245"
     CDS             complement(25835..27640)
                     /gene="sdhA"
                     /locus_tag="PKN51_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNT84145.1"
                     /translation="MSYTKDQVITRKFDVVIVGAGGSGMRASLELARAGLNVACLSKV
                     FPTRSHTVAAQGGVSASLGNMSEDNWHYHFYDTIKGGDWLSDQDAVEFMCREAPNVVI
                     ELEHFGMPFDRNPDGTIYQRPFGGHTANYGEKPVQRACAAADRTGHAMLHTLYQKNVE
                     SKTNFFVEWMALDLIRNSAGDVVGVTALELETGDLYELHAKAVLLATGGAGRIFAAST
                     NAFINTGDGLGMAARAGIPLQDLEFWQFHPTGVAGAGVLLTEGCRGEGAILLNSNGER
                     FMERYAPTLKDLAPRDFVSRSMDQEIKEGRGCGPNKDYILMKLDHLGADTIRKRLPSV
                     EEIGHNFANVDITKEPIPVVPTIHYQMGGIPTNIHGQVVTWDGEKNNIVNGLYAVGEC
                     AAVSVHGANRLGTNSLLDLLVFGKSAGKHIVQFVRSYGEQAAVPADASERTLARLNQL
                     ENSTDGIYAQDLANEIRQTMQQHAGVFRTQKGMDEGVVKIAGIRERVGQVTLKDKSRV
                     WNTARMEALEVDNLIEVAQATMVSAAARHECRGAHTVYDYEHPADHPTAPLGRDDANW
                     LKHTLWDSATNSLSYKPVNMKPLTVDSIPPKVRTF"
     gene            complement(27686..28051)
                     /gene="sdhD"
                     /locus_tag="PKN51_03250"
     CDS             complement(27686..28051)
                     /gene="sdhD"
                     /locus_tag="PKN51_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNT84146.1"
                     /translation="MSTTYGNKRLVVGAHYGWRDFLVQRISGALIAAFTLIVLLQVLF
                     TQGPIGYDTWAGIFAAQWMKALTFSVIVALIWHAWIGMNSIWLDYVKAAGVRLLLQTF
                     TVIWLVSCGGWALQALWRL"
     gene            complement(28075..28506)
                     /gene="sdhC"
                     /locus_tag="PKN51_03255"
     CDS             complement(28075..28506)
                     /gene="sdhC"
                     /locus_tag="PKN51_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNT84147.1"
                     /translation="MSEATKPRPEFRNINIFNDVRTYRLPLAGWVSILHRASGALMFL
                     LLPFIIWLFDKSVSSEISFGQFSAAFSSGLGAFPAWFVKLVVLALIWAYLHHLLAGVR
                     HLYLDISHRTTKEFGRQSAVATLVVSIVLTLALGAKLFGLY"
     assembly_gap    28651..28750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28765..29571)
                     /locus_tag="PKN51_03260"
     CDS             complement(28765..29571)
                     /locus_tag="PKN51_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT84148.1"
                     /translation="MAHAAPSSTPTESAPPALAPATAWGGQAPAFSPLYQQIKSLILQ
                     SLQQGEWKPGEVIPSEFDLAARYKVSQGTVRKAVDELAADNLLVRRQGRGTFVATHAE
                     QQVQYRFLRLQPDRGTADSEGPAQRDFVDCKRQRASADVARLLGLRAGDPVLQARRLL
                     RFAGVPTILEDLWLPAGPFKGLTAERLSQYHGPMYALFEAEFGVRMVRADEKLRAVLP
                     DPTQAALLEVTPATPLLSVERIAYTYNDEPMELRRGLYRTDTHHYRNALS"
     gene            29760..30746
                     /locus_tag="PKN51_03265"
     CDS             29760..30746
                     /locus_tag="PKN51_03265"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNT84149.1"
                     /translation="MSKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQKALKGVMMELEDCAFPLLAGMQAHSDPMTAFKDTDYALLVGARPRGPGMERA
                     DLLAANAQIFTAQGKALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPARNFTAMLR
                     LDHNRAASQIAAKTGKAVKDIKKLAVWGNHSPTMYADYRFATIDGASVKDMINDQEWN
                     ANVFLPTVGKRGAAIIDARGLSSAASAANAAIDHMRDWALGTGGEWVTMGIPSQGWYG
                     IPKEVMFGFPVTCENGEYKVVEGLPIDAFSQQCIDKTLAELEGEKDGVKHLL"
     gene            30897..31883
                     /locus_tag="PKN51_03270"
     CDS             30897..31883
                     /locus_tag="PKN51_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNT84150.1"
                     /translation="MTAVARHPRQVLLGAQAGAVVLPVCDHYSGVEARMRKSLQLQAE
                     MTQEFGTCVFDVTLDCEDGAPVGGEAEHAQCVTELALAAAPDARVGVRVHPVDHPAFE
                     ADMAQIAGKAGHRLAYIMIPKVESVEDVVHAERALRASFAPGLPLQVLIESPAAVHRA
                     FDIAAHPGVQSLSFGLMDFVSAHAGAIPAEGMGIEGQFTHPLVLRAKLEIASAAHAFG
                     KVPSHCVVTEFSDVQAMRVAARRAAREFGYARMWSIHPSQIRPILEAFAPDDRQIDLA
                     SAILLAAQQADWAPISHQGALHDRASYRHFWQVLERAHATGRHLPGDVAHWF"
     gene            31940..32527
                     /locus_tag="PKN51_03275"
     CDS             31940..32527
                     /locus_tag="PKN51_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84151.1"
                     /translation="MKPFAMTSLAIVLGLSMGAVQVASAADQPAPAKKVTTQAKKAPT
                     KKVPVKQAATSKGVPAKTAKAVEANTPVQTLSQRLTDTELEIAKTIQTGRIQCELGAD
                     VTVSADEKNPGFFHVVAGKQRYYMHPVESRTGAVRMEDNRAGAMWLQLGNKSMLMNQK
                     LGQRVADECVTPAQREFALQMKSSNQPGLLDGQTK"
     gene            32640..>33415
                     /locus_tag="PKN51_03280"
     CDS             32640..>33415
                     /locus_tag="PKN51_03280"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803432.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HNT84152.1"
                     /translation="MLQAYRAHAAERAKLGIPPLALDAKQVAELIELIKNPPAGEEQF
                     LMDLLTHRVPPGVDDAAKVKASFLAAVAHGDIQVGLISKAKATELLGTMVGGYNVHPL
                     IELLDDAEVAQVAADALKKTLLMFDYFNDVATKAKAGNAKAKEVMQSWADAEWFTSRP
                     KVAEKITVTVFKVPGETNTDDLSPAPDATTRPDIPMHYLAMLKNTRPDAAFKPEQDGV
                     RGPIQFIEDLKKKGHIVAYVGDVVGTGSSRKSATNSVIWAT"
BASE COUNT         4744 a        10848 c        12088 g         5635 t
ORIGIN      
        1 tggagcgaac tttcggggtt cgcgacgatc aggggtaaca tgacacgcat gttctttcac
       61 tccctgcctc cacccgctcc ggtcagcggc ggcgccgacg gtctgctggc gcgcgtgctg
      121 ggcgcgctgg tgtggctgat gctggccgcg ctgggggcgg tgtttgccct cagtctgctg
      181 atctggctgg tggtgatggt ggtggtcagc ctggtgggca gcctgttcac cggccggccg
      241 gccacggtga ccctgctgtg gcgccgctac cgcgacatga cgcgcgaggt gacgcgcagc
      301 cgctggtcgc cgtcgcccgt tagcagcacg ccgcgcgccg acgcggccca ggccaccggc
      361 gccgccagtg cccagacgtc ggtgcaggac gtgtcgtggc gcgaggtacc ggccgcgccg
      421 gccgccgacg gcgacgcgcc ggggcgctga aacccctcca ccgttgctac attaacaata
      481 gctgcctgcg ctttccgggt aagcgctgga tgccgatctg accacgattc ggccggtcag
      541 aaggcgggca tgagaggccc gcccacagct gttcaggcct cgtggcgcag cgccgggaac
      601 aggatcacgt cacggatgct ctggctgtcg gtcagcagca tcatgaagcg gtcgatgccg
      661 acgccgcagc cgccggtggg cggcatgccg tattccagcg cgcggatgaa gtcctggtcg
      721 aagtacatcg cctcttcgtc gcccgcgtcc ttggcgctgg cctgggcggc aaagcgggcc
      781 gcctggtctt cggcgtcgtt cagctcggaa aaaccgttgg ccatctcgcg gccggtgatg
      841 tagagctcga agcgctcggt cacctcgggc cgctggtcgt tggcgcgcgc cagcgggctg
      901 atctcggtcg ggtgctccat gatgaaggtg ggctgccaca ggtgctgctc cacctcttcc
      961 tcgaagtaca tcacctgcag gctggccagg ctgcgctggc tcaggcggtc cttgtcctcg
     1021 ctcaggccga gctttttcag gcgtcggatc agctcggccg catcgtcgac gtgggcgccg
     1081 atgtcggtgt gcgtgtgcag cgcctgacgg atggtcatgc gctggaacgg ctggctcagg
     1141 tccaccgccc gaccctggta gctcagttgc agcgagccgg tggcctgttg cgcggtgtcg
     1201 cgcagcagcg cctcggtgaa gtccatcagg tcctggtagt tccagtacgc ggcgtagaac
     1261 tccatcatcg tgaactcggg gttgtgccgc acgctgatgc cttcgttgcg aaagctgcgg
     1321 ttgatctcga acacgcgctc gaagccgccc acgatcaggc gcttcaagta cagctcgggc
     1381 gcgatgcgca ggaacatctg ctggtccagc gcgttgtggt gcgtcacgaa gggcttggcg
     1441 ttggcaccgc cggggatggg gtggagcatg ggcgtttcca cctccatgaa gccgtgccct
     1501 gtcatgaagg cgcgcatcgc cgacagggtg gcgctgcggg cagcaaagcg ggtgcgcgcc
     1561 tcgccgtcca tgatcaggtc gacgtagcgc tgacggtact tcagctcctg atcctgcacg
     1621 ccgtggaact tgtcgggcag cgggcgcagg ctcttggtca gcaggcggat gcgcgtggcg
     1681 tggatcgaca gctcgccggt gcgggtcttg aacaggtggc cctcgcaggc cacgatgtcg
     1741 cccaggtccc agtgcttgaa ggcctcgtaa tcggcctcgc cgacggcttc gcgcgcgacg
     1801 tagatctgga tgcggccggt ggcgtcctgc aaggtggcaa aactggcctt gcccatcacg
     1861 cgcttgagca tcatgcgccc ggccacgctg gcgtgccgct ggcggccggc cgccaggtcg
     1921 gcctcgaata cttccttttc gatagcgccg tgcgcttgct ggagctgcga ggcgcggtct
     1981 tttggcttga agtcattggg aaaggccacg gccccgccgg cggcctgcac ggcgcggatg
     2041 gcgcgcagct tgtcgcgccg ctcggcgatc agcttgtttt cgtcgtgttc ggaagcggcg
     2101 gcttgcaggt cggcgggcgc tgcgggctgg ttcatggcgt gtcgggcagg gctggggcgc
     2161 gagcagggca tgggcggcag cgataattcg ccgcgaccct tgccgatgtc gagggaaacc
     2221 gtctatttta ggcgcgcgcc gcccgtgacc ctgccgctga cccgtttccg cccgtcgacc
     2281 ccgcgcaccc ctgccggcgc ctgcccgcgg gcgccggcgg gcctgggagc gccgcggtga
     2341 aggccgcctc cggctcgcag ccgtcgctga cccgggccac gctcaccctg ttgggcggcg
     2401 gcgcgctggc gcagctgctg ccgctgctgc tggggccgtt catcgcccgc ctgttcacgc
     2461 ccgaagcgtt cggcctgtac acccggtttg ccaccgtggc ggcggcgctg gcggtggccg
     2521 ccagtctgcg ctatgagcag gcgctgccgc tggcacgcag cgacgacgag gcgcgcgcgc
     2581 tgctggcgct ggcgctgcga ctgctgctgg cggcggtgct gctgggcgtg ccgctgggct
     2641 gggcgctgca cgcgctgggc gccttgccgc tggcggtgct gctggcgccg gcgctggcca
     2701 cggccggcct gctgcaggtg ctgatgctgt gggccaatcg ggcccagcgt ttcggtgccc
     2761 tggccaccag ccgggtgctg cactacggcg gcgcggcggc gctgcaactg gtgctgggct
     2821 gggccctgtg gcgcggcgcg gccagcgggg ccgagggcgc ctgggcgctg gcgctggctc
     2881 cgctgctggc gcagctgctg gcctgcggcg ggctgtggcg gcccgcgccg gccggcggct
     2941 ggcgggcgct gctggtgccg gcgtcggccg cgctgcgcac gcagatgcgg acggtggcgc
     3001 ggcagcagcg cgactttgct ctcatcaaca cgccgcacgc cctgctgggc ctgctgcagg
     3061 acgcgctggc ggtggcgctg ctggtggcct ggtcgggcga ggcggcggcc ggcttctggg
     3121 gcctggcgct gcgctacctg aaggcgccgg ccacgctggt ggggggggcg gtgtcgcagg
     3181 cgctgtaccc gcgcctggcg gcggcccggg ccgacgacgc gcgtcgcctg gtgcgccggc
     3241 tgatggccct gctgggggcg ctggccctgg ggctgatggc gctgctgctg ctggccgggc
     3301 cgtggctgtt cgagcgcgtg ttcggcccgc cctggcgcga ggcgggcgag ctggcgcgtg
     3361 cgctggcgcc ctacatcgcg gcgcatttcg tggccgcgcc gctggcggtg gtgaccatgg
     3421 cctggcgcgc ccagcgctgg gcgtttcggc tggcgctgct gggtcagctg gcgtttctgg
     3481 ccgcgctggc gctgggtctg cgccaggggg ggctgctggg cggcgcctgg gcggtttcgg
     3541 cggtgatgct gccgtacttc ggctggtact tctggcgcct ggccggctgg cgcgacattc
     3601 catcgcccct gggcgtgccg ccgcgcgagg gtgccccatg accgcgctgc gtgccgcgct
     3661 caaccgtctg cgccgccggc tgtggcggct gctgtttcac cgcatgctgt tcggcgaagc
     3721 cagtgccggc gttctgcgcc cggccacgcg cctgtcgccc acggtgtgca tcgagcacga
     3781 gccgacgctg acgctggccg acgacgtgtt catcggccat ttctgcttca tcgaggccag
     3841 tggcggcgtg cgcatcggtg gcggcacgca ggtgaccaac ttcgtcagca tcgtcagcca
     3901 cagcacgcac cgcgcgctgc gggtggcggc cgggttgtcg ggcgcgccgc tggaggcggc
     3961 ggtgcggcgc gcgccgatcg tgatcggtga acgctgcttc atcggcccgc acagcaccat
     4021 cgaggccggc agcacgctcg gccacggcac gctggtggcg gcgcacagcc gggtgcgtgg
     4081 ggtgtttccg gacttcgccg tgctggccgg cagcccggcg caggtggtgg gcgacacgcg
     4141 cgcggccgac gagcgctggc tggcggccca tcccgaactg ctggcggcgt atcggcgctg
     4201 gcgccagggg ctggcgcagt gacggcgccg catctgggcc ttctgggcga cgccagcagc
     4261 gtgcacgtgc agcgctgggc gcgtgagatg cgcgcgcgcg gctggcgcgt gtcgctggtg
     4321 acggcgcggc cggcgcccat cgagggcgtg tcgcagatcg tgttgcgccc ggtgcggcgc
     4381 gccagcgact ggctgtggcg cgtgggcgag gcgcggcgcg cgctgaccca gttgcagccc
     4441 gacctggtgc atgcgcacta cgtcacctcc tacggctacc tggcggcgcg cgccggcctg
     4501 caaccgctgg tgctcacggc ctggggcagt gacctgctgg tcacgccgcg acagagcgcg
     4561 ctcaagcgct ggctgacggc ctggagcctg cgccgcgcac gcctggtgac gggcgactcg
     4621 cgcgacttgc tggctgcggc gcgggcgctg gcgccgggtg tgccgaccca gctggtgcac
     4681 tggggcgtgg agcgcagccg cttcgcgccc gtgccctggg ccgacaagcc ggcggccgag
     4741 gccgtgagcc cgcgcaactg ggagcccaac taccgcatcg acgtcgtgct gcgcgcgctg
     4801 gcgcaggtgc gcgccgtgcg cccggaagcg cgcctgcacc tgctgggcgg tggcccgctg
     4861 gaggccgatc tgcgcgccct gaccggacag ctggggctgc aggacgcggt gcgcctgcac
     4921 ggccggctcg acgacgccgg catggccgcg gtgctggcgc gctgcaagct gtcgatcagc
     4981 gtgcccgaga gcgacgccac ctcggtgtcg gtgctggaga gcatggcctg cggcctggcg
     5041 gtgatcgcca gcgacctgcc ggccaaccgc gactggctgg cgcccgaggc gctggtggcg
     5101 gccggcgacg tggcggcgct ggcggcgcgc tggctggcgc tgctgcagga cgacggccgc
     5161 gccgcggtgc tgggcgcggc caacgccgaa cgcatcgcgc gcgacgggga tcggcgcgtg
     5221 cagatggacg ccatcgacgc cgcctgccgg cgcctgctgc aggaggccgg ccgatgacgc
     5281 aggaactggc cacgccgccg ctgccgcagc tgctgacgcc gggcgaagcc gggctggtga
     5341 gcgtggtggc gccgtgccgc aacgagcggg cccacatcga cgccttctgc gactcggcgc
     5401 tggctcagcg cctgcccgaa ggctggcgca tggagctgct ggtggccgac ggcgccagcg
     5461 acgacggcac gcgcgcgcgg ctggccgagc gcgccgccgc cgatgcccgg ctgcgcctgc
     5521 tggacaaccc gcggcgcatc gtctccagcg gtctgaacgc ctgcctggcg caggcgcgcg
     5581 ggcaggtgat cgtgcgcatg gacatccaca cccggttcgc ccccgactat gtcgccgaat
     5641 gcatcgccgc gctggcgcgc agcggtgccg acaacgtggg cggcccctgg gtggcgcgcg
     5701 ggcagggcgg ctgggggcgg gccatcgcgg cggcgttcca gagccgctgg gtggtgggcg
     5761 gggcgcgctc gcgcgatgcg gcgttcgaag gcgaggtcga taccgtctac ctgggctgct
     5821 ggccacgcgc gagcctggcg cgcgtcggcg gctttgacga aaccctggtg cgcaaccagg
     5881 acgacgagca caacctgcgc ctgcggctgg ccggtggtcg catctggcag agccggcgca
     5941 ttcactccag ctaccggccg cgcgattcgc tgcgccagct cttcgagcag cagctgcaat
     6001 atggctactg gcgccccttc gtgctgcgca agcaccggca ggcgggctcg gtgcggcagc
     6061 tcgttcctat ggtttttgta gctgctggcg ctttctgcgc gctgcttgcg ccggtgtttg
     6121 gtcttggtct gaaggcctgg gctgcggcct atgccgccta cctgctggca gccagcgcgc
     6181 aggcggcgca ccaggccggc gaggcgcgtc tggcgtggcg gctgccgtgg gtgatcgccg
     6241 cctaccacct gggctatggc ctgggcagct ggcgcggcgt ctggggcctg tggcgccggc
     6301 gcgccgcccc gcaggcggcg acccgcctga cacgctgaca caggcgccgt ggcgcgcagc
     6361 gcagacaata gcggcttcat gagcgtttcc gaaagctttc ttccctttgc ccgccccgac
     6421 atcggtgagg ccgaaatcga cgccgtggcg cgcgcgctgc gctccggctg ggtcaccacc
     6481 gggcccgaga cgcgcgcctt cgagcaggag ttcgccgctt tcctgggcgg cggcgtgcag
     6541 gcgatcgccg tcaattccgc caccgccggc ctgcacctgg cgctggaggc gatcggcatc
     6601 ggcccgggcg acgaggtgat cgcccccacg ctgaccttca ccgccaccgt cgaggtggcg
     6661 cgctacctgg gcgccgacgc ggtgctggtg gacgtcgatc cggtcaccct gaacatcgat
     6721 ccggccgcca tcgaggccgc catcacgccg cgcacgcgcg ccatcctgcc ggtgcactac
     6781 ggcggcctgg cctgcgacat ggcggccatt cacgcgatcg cgcgtcgcca cggcctgcag
     6841 gtggtcgagg acgcggcgca cgcgctgccc gccagctgcg gcggcacgcc ggtggggcag
     6901 ttgcccagcg cggctacggt gttcagcttc tacgccaaca agaccatgac caccggcgag
     6961 ggcggcatgg tggtgacgcg cgacgaagcg ctggccgcgc gcatgcgcgt gatgcgcctg
     7021 cacggcatca gccgcgacgc cttcgaccgc ttcaccagcc gcacgccggc ctggtattac
     7081 gaggtggtgg cgccgggctt caagtacaac ctgaccgacc tcgccgccgc gctcgggcgc
     7141 gtgcaactgg cgcgcctgcc ggccttcgtg caacggcgcc agcaactggc ggcgcgttac
     7201 ctgcgcgagc tggccgggct gccgctggtg ctgccgcccc aggcgccggc cggcgacacg
     7261 cacgcctggc acctgttcgt gctgcgcctg gccgacaccg cgcgggtgac gcgcgacgag
     7321 gtggtgcagg cgctggcgga cgccggcatc ggcaccagcg tgcatttcgt gccgctgcac
     7381 cgccagccct actggcgcga tcgctatgcg ctcaggccgc aggattttcc ggtggccgag
     7441 gcggcctacc agcgcatgct cagcgtgcct ctgttcaccg ccatgagcga tgcggagcag
     7501 gggcgcgtga tcgcggcgct gagggcctgc ctgcaatgac gccggcccgc ctggctgctt
     7561 cgccctggcc ctggtttggc tggagcgcgc gcgcctgggc gcggccctgg cgtgcgttcg
     7621 tgcgcgactt cgccggctgg cagggcgagt gcgggctgga gctcggcgcc ggtccgcgct
     7681 cggcgctggc gccgttgatg ctgagcctgg tgcggcaggt ggaatgctcg gcccaggacg
     7741 cggccagcct gccggtcatc gaggcgcgcc accgcgacct tctggcaccg caggacgccg
     7801 cccgcatcgc ccacggcgtg cgagatctgc gggcgctgca cgggcaatgg gacctgatcg
     7861 tcatgaagtc ggtgctgggc ggcgtgcacc ggctgggccg ctcctcgctg gccgacgtgc
     7921 acaccaccct ggagcaactg gtggcccggc acctgcgccc gggcggcctg ctggtgacgc
     7981 tggacaacgg gcgcaccgcg ctggagccgc tgtggtcgcg ctttggcgcg cgccgcaacg
     8041 gctggcgctt tttcacgcgc gacgagttcc cgcccgcggc ggcggcgtac ggcttcggcc
     8101 tgctgggcag cttctcggcc gccacccgcc tgggtcggcc cgggcgctgg agcgacgacg
     8161 cgctgtacgg cgccgatctg ctgctgtcgc cctgggtgcg gcgccacgcc gtgctgctgc
     8221 acgtctaccg caagccggca tgagcaccac gagcaccgac gccgccaagc gcgtgttcga
     8281 cctggccctg gcgttgccgg ccctgctgct ggcgctgccg ctgatggccg ccatcgcgct
     8341 gtgggtgcgg ctcgactcgc ccgggccggc gtggttccga caggcgcgtg tggggcgcgg
     8401 cgggcggctg tttcgcatcc acaagttccg caccatgcag gtgcaggccg tggccggcgg
     8461 gcccctgatc accgtggccg gcgacgcccg cgtgacgcgg gccgggcgct ggctgcgacg
     8521 caccaagctg gatgagctgc cgcagttgct ggatgtggtt cgcggcgaca tgagcctggt
     8581 cggcccgcga cccgaggtgc cgcgctacat ggcgctgtac ccggacgagg cgcggcgcct
     8641 gatcctgtcg gtgcgccctg gcctgaccga ccgggcgtcg atcgaatttc gggacgaaga
     8701 gcggctgctg gccgccgcca gcgatcccga gcgtgcctac gtcgagcagg tcatgccgat
     8761 caagcagcgc tactacctgg actacgtggc gcagcgcagc ctgaccggcg atgtgggcat
     8821 tctgtggcac acgctgcggg ctgtgctgcg gcgtccggat tccagatgaa atcggccttt
     8881 gacgcccgtc tgtcatgcgc cgcaagctat aaaaagaata gtatccggaa gcctcagcgg
     8941 ggcgcgccgc cgatgccggt gcggctcagc agatcgacca ccagctccag ccgcagcagg
     9001 ggactgtcca ggctcatcag gtgctgacgc gtggcggtgg gcagcggcag cagctcgcac
     9061 cagcggttgg ccacccagcc gcagtcgtcg tagtccagcg cgtcgccgcc gggcagccgc
     9121 aggccctggg gttgctggcg cagggtgtcg tgcgcgcggc gcagggtgcg ggcggcggcc
     9181 tgcaggtcgt ccggaatggt caccaccggg tcgggcggca gcaggctgac gtcggccacc
     9241 cacaggccat ggcgcagctg gtgcggctgg tcgatgcgaa agcgctgttc gccctggcat
     9301 tcgatcagca tcagccccgg ctgcggttgc gtcagcgtgc gcacatgcgc cagcgtgccg
     9361 accggctgca gccgctcctg cggtgcgccc ggggtgcgca cttcctgacc ctgcgccagc
     9421 gccaccacgc cgaacggcgc gccggcgcgc tggcagcgcc cgatcatgtc caggtagcgc
     9481 acctcgaaca cccgcagcgg cagccagccg cccggaaaca gcacggtgtc gagcggaaac
     9541 agcggcaggc tggtgagggt gagcggagcg tcggccatgg ggcagggtgg tcgaccggcg
     9601 ccgcggggcg ggcggacggc ggccgctatc atcgcacgcg gggatcggcg tgctgtgggc
     9661 ctggacgcca ggccgtgccg atcccgtggc cgtcgccccg catgctctac acgatccttt
     9721 cctttctgct cgaaatcggc gtcaccctgg tcgggggcgc ctgcctgctg cggctttaca
     9781 tgcgctggcg gcgcatgagc ttcgacaacc cggtgggcca gttcgtgctg gcgctgtcgg
     9841 actggctggt gcagccgctg cagcgcgtgg tgccgcccgc cggcgggctg gacgtggcca
     9901 gtctgctggc tgcctggctg ctcaagctgg tgcagtacgc gctgctgatg gcgctgctgg
     9961 agctgccgcg ctggggtctg ctgccggtgc tggcgctgct gggcacggcc cggctggcgg
    10021 tgtcggtgtc gacggcgctg gtgatcatcg cggccgtgct gtcgtggacc ggccagcgca
    10081 gccgggcgca cgacgtgttc gagcgcctgt gcgcgccgct gctggcgccg atccgccgcc
    10141 gcatccggct ggtcggcggg gtggatctgt cgccgctgct gctgatcgtg ctgctgcagg
    10201 tgctgggcat cgtgctcagc tcgttgcagt cgggcctgct gggtccgggg gtgccggcgc
    10261 tggctggctg atgcggctgc gcgttcacat gcatgaccca aaggtcattt tgaacgcgag
    10321 ctcgcatgag gcgctgcccg ctcggccctg cggcccgctg gccggttctg ccaccacccc
    10381 gaattgctat caatgtgata gctttcggcg ctgcacccat gggcgctgca gcctgttttt
    10441 ttactcgacg gcgtcggccg gctggcgctg cagcatcgcc aggatggagg ccaccttctg
    10501 gcgcagttcg cgccggtcgc agatgaaatc gaccgcgcct ttctgctgca ggaattcggc
    10561 acgctgaaag ccttcgggca gggtcacgcg cacggtgttt tcaatcacgc gcgggccggc
    10621 gaagccgatc agcgccttgg gctcggcgat caccacgtcg ccgacgaagg cgaagccggc
    10681 cgacacgccg cccatggtcg ggtcggtgag cacgctgatg tagggcaggc ccttcttggc
    10741 cagctgggtg agggcggcgt tggtcttggc catctgcatc aggctgaaca ggccctcctg
    10801 catgcgcgcg ccgccggtgg cggtaaagca gatgaagggc actttctgct cgatcgcggt
    10861 ctgcacgccg cgcacgaagc gttcgcccac cacgctgccc atggagccgc ccatgaagtc
    10921 gaactcgaac gccgccgcca ccacgctgat gctcctgacg gcgccgccca tgacgaccag
    10981 ggcgtcggtt tcgccggtgt tttccagcgc ctccttcagg cgctcggggt acttgcggct
    11041 gtccttgaac ttcagggcgt cgaccggcac cacctcctgc ccgatctcgt agcggccctc
    11101 gccgtcgagg aaggcgttca ggcgctcgcg cgcgccgatg cggtggtggt ggctgcagtg
    11161 cgggcagacg ttctggttct gctccaggtc ggtcttgtag aggacggtgt cgcagtccgg
    11221 gcacttgatc cacagcccct cgggcatctg gcgccgctcg gcggcgtcgc ttttctggat
    11281 cttgggcggg aggagttttt cgagccagct catggcgctt tccttcttga gtggcgccga
    11341 ttatgcgtcc agcgcggcgc gcatcgggcg cagaaaatcg gccgcggcgg cggccacgcg
    11401 ctcgtgtggc tggttttcga tctgctggat cagccggctg ccgatcacca ccgcgtcggc
    11461 cacctgaccg atggcgcgtg cggtctcggc gtcgcggatg ccgaaaccga caccgaccgg
    11521 aatgtggacg tgctggcgga tgcgcggcag catggcgccc acctcggcgg tgtcgagcgc
    11581 gcccgagccg gtgacgcccc tgagcgagac gtagtacacg tagccgctgg cgacttcggc
    11641 cacctggcgc atgcgcgctt cggtggaggt gggcgccagc aggaagatca ggtccagccc
    11701 ggcggcgcgc aggcgggcgg cgaagtcacg gcattcctcg ggcgggtagt cgacgatcag
    11761 cacgccatcg acgccggcgg ccgccgcatc gcggatgaag gcgccatcgc cgtgcttctg
    11821 gtcgtagcgt tcgaccgggt tggcgtagcc catcagcacc accggggtgt cgccgtcctt
    11881 ggcgcgaaag ctgcgcacca tggccagcac ctggcccagg ccgatgccca gcgccagggc
    11941 acgttcgccc gccttctgga tcaccgcgcc atcggcgctg gggtcggaaa acggcacccc
    12001 cagctcgatc acgtcggcgc cggcggccac catggcgtgc atcacggcgg gcgtgatgtc
    12061 ggcgtacgga aaaccggccg tgacgtaagg aatgagggcc ttgcggccct gggcctgcag
    12121 ggcttcgaag gtggcagcga tgcggctcat gccctggccc ccttcaccac gtggccgcgc
    12181 atggaggggc ggtcgtagaa gtcggcgccg tcgagatcgg ccacggtgcc gatgtccttg
    12241 tcgccccggc ccgacaggca caccagaatg gattggtcgg gtcgcatggt gggcgccagc
    12301 ttcatggcgt gggcgaaggc gtggctggac tcgagcgccg ggatgatgcc ctcggtgcgg
    12361 cacaggtggt gaaaggccgc cagcgcctcg gcgtcggtgg cgccgacgta ttcggcgcgg
    12421 ccggtgtctt tcagccaggc gtgctcgggg ccgacgccgg ggtaatccag gccggccgag
    12481 atgctgtgcg tctcggtgat ctggccgttg tcgtcctgca ggatataggt gcggttgccg
    12541 tgcagcacgc cggggctgcc gcgctgcagg ctggccgagt gcttgccact gtcgaggccc
    12601 tggccggcgg cttcgacgcc gatcaggcgc gtgttttcga acgggatgta ggggtggaaa
    12661 atgcccatgg cgttgctgcc gccgcccacg caggcgagca cggcgtcggg ttgccgggcc
    12721 gccatgtgct gctcggccag cagctcaggc atctgcgtca ggcattcctg gccgatcacg
    12781 ctctggaagt cacgcaccat catggggtag gggtgggggc cggcgacggt gccgatgatg
    12841 tagaaggtgt ggtccacgtt ggcgacccag tcgcgcatgg cctcgttcag cgcgtccttg
    12901 agcgtgcggc tgccgctttc caccggcacc acggtggcgc ccagcagctt catgcggtag
    12961 acgttggggc tctggcgctt gacgtcttcg ctgcccatgt agaccacgca ctgcagccca
    13021 tagcgcgcgc agatggtggc cgtggccacg ccgtgctggc cggcgccggt ttcggcaatg
    13081 atgcgcggct tgcccatgcg cttggccagc atggcctggc cgatcacgtt gttgatcttg
    13141 tgcgcgccgg tgtggttgag gtcttcgcgc ttgaggtaga tctgcgcgcc ccccagctca
    13201 cggctggtgc gcgcggcgcg gtagatcggc gaggggcggc ccacgtagtg cgccagctcg
    13261 tggcggaatt cgcgctggaa ttccgggtca tgctggtacc tggcgtaggc gtccctcagc
    13321 tcgttgatgg cgtaggtcag cgtctcgctg atgaagctgc cgccgtagcg gccgaaatgc
    13381 ccgctggcgt cgggctggtg gtagtcactc atgtcggggg cgatcctgca agggcggcgt
    13441 cggccgcacg cacggcggcg acgaactgaa aaatgcgggc agcgtccttg atgcccttca
    13501 ggggctggcc gtcggggccg acggcttcga cgccgctgga gacgtcaacc gcgagcgagc
    13561 gcccgcggcc gcgcagcagc cgcaggccat cgcccacatt tgcaggtgtc aacccaccag
    13621 acaagacgag gtgagcggcg acgtttggag gcagcagtga ccaatcgaat gtctttccgc
    13681 cgccgccgaa gccctcgaca tgggcgtcga gcagcagggc ctgtgccgtg gcgaaggcgt
    13741 gggcaaattc tatcaagtcg aacgcgccgg cggcgcccgc agggatgcgc gccgcgcgcc
    13801 agtacgggcg cccgcagcgg gccgccaccc gggcgcagtc ggcggctgat tcatcaccat
    13861 gaaattggag tgtagcgccc gtcaggagcg cgcaatcagc tatgatttct gaagcatcgg
    13921 cgttgacgta gagcagcacc ggcgtgacca tcggcggcaa ccggcgcgcc agttcggcgg
    13981 cgcgccggat gtcgacgtgg cgcgggctcc tggggtagcg cacgaagccg atcgcatcca
    14041 ctcccgcggc cacggcggcg tccacgtcct gcgcgcgcgt caggccgcag atcttgatgc
    14101 gggtgcgcgc cccggcgagc gcaagggaaa cgggtgtcat gatggcagcc aatcataggc
    14161 cggcgtgcgc tcgggcaagc cccagcgggc gtcgtagcgc ggaccgagga aatacagtcc
    14221 gtcgggcgca aacgtgggcg ccgccgcatc gcggctgcgc gcggccagca cgctgtcgat
    14281 ccactcgggc gggtgcaggc cctggccgat cgccaccaga cagcccatca ggttgcgcac
    14341 catgtggtgc aggaaggcgt tggcctcgaa gtcgaagcgc cagtaggcgc cgcagcggcg
    14401 gatgtcaatg cgctgcagct gcttgacggg ggtcagcgcc tggcaggcgc tggcgcgaaa
    14461 cgacgagaag tcatgttcgc cgatgagtcg gtcggccgcg cgccgcatcg ccacgccatc
    14521 caggggccgg aaggcccagc cgacgcgccc gctctccagg ctggggcgca ccggcgcttc
    14581 cagcacgacg taggtgtagc ggcgcgccag cgcgctggcg cgcgcatgaa actcctgcgg
    14641 caccggctgc gcccactgca cggcgatgtc gcgcggcaga aagcggttgg tgccgcgcac
    14701 ccaggagaaa gcatcgcgct caagctcggt gtcgaaatgc accacctgca tcaggccatg
    14761 cacgccggcg tcggtgcggc cggcgcacag cgtggcgatg ggggaggtgg caaatgcggt
    14821 cagcgccgcc tccagccggt cctgcacggt ctgcccggtg ggttggcttt gccagccctg
    14881 gtagggggtg ccgagatagc tgaggccgag ggcgattctc acgatggcgt gattgtggac
    14941 gaagcgtggg ctccaggagg gctccaaaag ggcgcggcgc ccatcggggc gccgcgttgc
    15001 cggagtccgg aggccaggcc tcggtgggga cggatcagtc gagctcggcc aggaaccgct
    15061 cggcccgcat gcgcaggctg ccggtggcct cggcgatcac ttccctgacc agcgtgcgcg
    15121 cgccctcggt atcgccgatg gcgtggaact cctgcgccag cgccagcttg gtggccaccg
    15181 gatcgtcgtc ggcatcgtcc ggttgttcgg ttttcggatc aaccccggag cgagagtccg
    15241 gatcgatcga cagggcctcc atgtcgaagt cgatcatgcc agccttgctc gccacatagg
    15301 gctgagcaac cgatggcggt tcgtcgtcgg cctgcgtggc aaccgcggca gccagggcgg
    15361 gggccaccgt ggtcggcgtg tctaggtcga ggtcgaagtc gggcagcgca atcggcacgg
    15421 gcttgacttc gggcgcggca gcgatcgcgg gctccgggcc gtggtgactc ggcatggcgc
    15481 tggtgttccc cacggacggg gggaccgacg tcaccgcgct ggcacgcacc ggggcgtcgt
    15541 ccaggtcgag ttccagatcc agcgggatgc tcgagctgtc gggcttggaa tcgcgcttgt
    15601 cctgcaattg cgccgtctgt ggctcggtat cggcaccgaa ccgcggctgc ggtgtagtgg
    15661 cggtggcggc gctccgcatc atgggaacac cgcccggctt gtagaggggg ttgtcgggat
    15721 cgagttcgct gccgaggctg gcgattgccg cccagtcggc accttctccc ttgacgtggt
    15781 tccaggcttc cgaggcgacc atttcgaggg cgcgggcgtc gcgccgtctg gcatagatct
    15841 ctgccagctt gcgatgcacg ctgacccgtc caggatgggt gcgcagtgct tctttgagga
    15901 tttcctcggc ctgcatgtca cgtccgtagg ccaggtagac gtcggcctcg gccacgggat
    15961 cgacgtcgcc ggctgcgtcg agttgactgg gcgaatagac catggacgag ctgctgccca
    16021 ccgacggccg accctcgcgc gtgtcgacgc gctgtccacc gctggcgccg aagaaggagt
    16081 cgggctgcaa acggctctcg atgaacgagc tgtccattgt ggcgtcgccc ttcttgctct
    16141 ggcgccagcg gtacacaccg aagcccgcca gcagggcgat caaacctgca ccggcgatag
    16201 gcagcagcgg gttgtcggtc agcgcatcga ggaaagacgg cccctcactc gcggtggtcg
    16261 gcggcacggg agctgcctgc ggcgccggag cggcaactgg cgctgtcacc gtttcggaga
    16321 cgccggaagc cggaaccgct gcaggttctg ccatggcggc ggaggcgggt gcagccggga
    16381 cggccgaagc cgcatcggcc attgtcggtg ccggtgccgg tgccggcgct gaagcgacgc
    16441 tggccggttc ggaggcgctg gctggcgtcg caacggtggg cgccgccggt tcggcggcga
    16501 cggaagccgc aggtgcggcg ggcgtcgctg cgacgggtgc ggctgcgggc ggagtgccga
    16561 tgggcaattt cacgcccgtc gaagtcgcgc tgcccgatgc ggcctgcagg cgcgccagct
    16621 cgctgagatt cttgttcaac tcggcgacgc gatcggattg ctcctgagcc tggcgggact
    16681 gcgccagttg tgactccgct cccgtttgtg caccctcgcg cgacagcttc aggcgatcgg
    16741 ggctgggcgc cgccgcgcgg ctgtcccgga cttcaggttg caccccaccg gacgcactgc
    16801 cagtggcggc cgtggccacg cgcgtggctc cgctgcgctg cgccaggcca agacggtagg
    16861 cgttgaagtc ccgcgtctgg gcaacgatgg cacgacgggc ggcttgccgg gtgactgcgc
    16921 ttgcctgctc gaccgacggc atctggagca cggtgcctgc ccgcagcaaa ttgacgttgc
    16981 cgttgatgaa cgcatcgggg ttggtgcgca gcatggcgac cagcatctga tcaagcgaca
    17041 cgccctcgat ggcatgcgcc cgcgcgatgc tgcctgcggt atcgccacgc tgcaccgtca
    17101 cgttggcgcc ttcaccggtg gtgctcaccg cctgtcgtgg cgcctcggcg ggatggatgt
    17161 cgcggcgtgg gcgcgagccc gcgtccgccg ccgcgacggg cgggaggggg gccgccctgg
    17221 gcgcatgcac cacggcagat gtcggtggcg ctgtggcctg cgaggcggtg acggagaccg
    17281 gtggaggcgc accacgaccc ggcggatcca ccagcaaggt gtagtcgcgc actacgcggc
    17341 cattggccca attggcctcg accacgatgc tcaggaaggg ttcgttgacg gtgcgttcac
    17401 cagtcacccg caagtaggct tgaccgctgg ctcgccgctg caggctcacg cgcgccccgg
    17461 ccagggcggc ggggaaatcc actccggcag cgcgaaacgc ttcgggtgag ccgatgttgg
    17521 ccttcagcgt cgcggcttct tcggaagaga tctgcggcac ctcgatctcg gctctcaggg
    17581 gctcaccgag tgccgagcgg accgtgatgg cacccagcgc cagcgcttgc gcgtccaggc
    17641 tcacgccgca cgtggcaatg gccacggccg aggccagcac gcctttgcgc caaggttggc
    17701 gaacgctgtg acttgaagca atcgacccga gcaaggtcat agcggtcttg ttctgcatgt
    17761 ctggtgttgg gcaggctgag cccggccgga acgaatgtgg aaaacgacat taacatcaac
    17821 gtattagcct gacaagctga ggcactgtct aatcctgcct gtagcgcctc actgcggtgc
    17881 aagggcactg agcttgttgc aatacgacaa cgggcagccc ccgtccaggg ccgcccgctg
    17941 cgagatccgc gccgctcgtc agtcctgcag caggatgcgc aacatgcggc gcagcggctc
    18001 ggccgcaccc cacagcagct ggtcgccgat ggtgaaggcg cccaggtatt gcggccccat
    18061 ggccatcttg cggatgcgac ccaccgggat ggtcagggtg ccggtgacgg ccaccggggt
    18121 cagatcgcgc agggtggcgt cgcggttgtt gggcaccact ttcacccagt cgttgtcggc
    18181 ggcgatcatg gcttcgatgt cggccaccgg cacgtccttc ctgagcttga aagtcagcgc
    18241 ctggctgtgg cagcgcatgg cgccgatgcg cacgcagtag ccgtccaccg gcaccgccgg
    18301 cgtgtcgaag ccttcgccct ggccgaggat cttgttggtt tcggccatgc ccttccattc
    18361 ttcacggctc atgccgttgc ccagatcctt gtcgatccag gggatcagcg agccgcccag
    18421 cggcacgccg aagttggctt tctcggcgtc gtccagcgcg cgctgcttgc cgatcacctt
    18481 gcgatcgatt tccagaatgg cgctggtcgg gtcggacagc aggtcgcgca cctcgtggtt
    18541 cagggtgccg aactgcgcca gcagctcgcg catgtgctgc gcgccaccac cgctggcggc
    18601 ctggtaggtc tgggtgctca tccattcgac caggccggcc ttgtagagcg cgcccacgcc
    18661 catcagcatg cagctgacgg tgcagttgcc accgatccag tcgcgcccgc ccttggccag
    18721 cgcgttctgg atcaccggca ggttgaccgg atcgagcacg atgacggcgt tcctttccat
    18781 ccgcagggcc gaggcggcgt cgatccagtg gcccccccag ccggcggcgc gcagcttggg
    18841 gtagaccgca tgggtgtagt cgccaccctg gcaggtgagg atgatgtcgc actttttcag
    18901 tgcttcgatg tcgtgcgcgt ccttcagctg gcgttcgttt ctggcctggg cgggcgcggc
    18961 gccgccggcg ttgctggtgg aaaagaagat gggctcgatc aggtcgaagt cccgctcggc
    19021 ctgcatgcgg tccatcagga ccgagccgac catgccccgc cagccgacga ggccaacaac
    19081 gttaccggtt ttcatttgct ttggcgcctt tcaaacaaag tagattcgtt ctgacctgcc
    19141 gggctcgtct ggctccggag gcggcgcgca cgacgaaccg aaggctgatc agcctttttt
    19201 ggtgatcgtg gttgtgcgga acgtggacac gccagcggcc ggcgcggtgg ccgggcggtg
    19261 cagatcgcgg ggatcgggcg tgtgcatggt gcgcagattc tagcgcaggc cggcgccgct
    19321 gcggcgactg cccctgcgcg gcacgcggca cctgcggcgt gggggcgaca gtggctggcc
    19381 ggcgcccgcg cctgcgcgcg gaatcatgaa caaaatggca actggcgctt acgcagcaag
    19441 cgccagaagc tattcaaatg atagcttagg aatgcttttg cggtgccggc tgaaccccgt
    19501 tcggcccctc gctgaccggc accgcggcgc ggcggcgctc aggtggtggg ccgggcgatc
    19561 ttcagtccag cgcccggacc acggcctcgc ccatctcgcg cgtgcccaca cgggtcgtgc
    19621 cttcgctcca gatgtcgggc gtgcgcaggc cctgggccag caccttcctg acggcggctt
    19681 cgatgcggtc ggcggcttcg ggctgaccga gcgagaagcg cagcatcatg gcggccgaga
    19741 ggatggtggc cagcgggttg gccacgccct tgccggcgat gtcgggggcg ctgccgtggc
    19801 tgggctcgta caggccctgc tttttgtcgt tcaggctggc cgagggcagc atgccgatcg
    19861 agccggtgag catggaggct tcgtccgaca ggatgtcgcc gaacatgttg ccggtgacga
    19921 tgacgtcgaa cgccttgggc gccttgacca gctgcatggc ggcgttgtcc acgtacatgt
    19981 gcgacagctc cacgtccggg tagtccttgt gcacctcggt gaccacgtcc ttccagagct
    20041 ggaaggtttc cagcacgttg gccttgtcga cgctggtcac gcgccgcgag cgctgacgtg
    20101 ccgcctcgaa ggccacgcgg gcgatgcgct cgacttcggg gcgcgtgtag cgcatggtgt
    20161 cgaaggcttc ttcggcgccc ggaaagtgcc catcgggggc ctggcggcgg ccgcgcggct
    20221 ggccgaagta gatgtcgccg gtcagctcac ggatgatcag gatgtcgagc cccgccacca
    20281 gttcgggttt gaggctgctg gcggccgtga gctgctcgta gcagatcgcg gggcggaagt
    20341 tggcgaacag gcccaggtgc ttgcgcaggc ccaggatggc ttgctcggga cgcagcggac
    20401 gatccagcgt gtcgtacttc cagtcgccca cggcgccgaa caaaatggca tcggcggccc
    20461 gggccagatt cagggtggac tcgggcagcg gatggcccgc ggcctcgtag gcggcgccac
    20521 cgacgggagc gctttccagc tcgagtttca ggcccagcgc ctgcagcacc ttgacggctt
    20581 cggaggtgat ttcggggccg atgccgtcac ccggcagaac tgcgattttc atgtggcaaa
    20641 gacttcagaa tggatagctg ccggcgcttg tctggtgcgc gctggcggcc gatttggctt
    20701 ggaaacaagg ggcgcggccg cggcgccgcg ccatcaggcc gggcggccca tggtgtgggc
    20761 cagccagggt ttgctggcca ggcgctcggc ttcgaagcgg gcgatcgcgt ccttgtgctg
    20821 cagcgtcagg ccgatgtcgt ccaggccgcc cagcaggcag tgcttgcgga aggggtcgat
    20881 gtcgaacgcc agctcggtgc cgtcaccctt gacgaccacc tgccgctgca ggtcgatggt
    20941 gagctggtag ccgggaaagg ccgccacgtc atggaacagc ctgtccacgg ccgactcggg
    21001 cagcacgatg ggcagcaggc cgttcttgaa gcagttgttg aagaagatgt cggcaaagct
    21061 tggcgccagg atggcgcgaa agccgtactg ctgcagcgcc cagggggcat gctcgcggct
    21121 cgatccgcag ccgaagttct ggcgcgccag cagaatgctg gccccctggt agcgcggctg
    21181 gttgagcacg aaatcggggt tgggtttgcg actggccggg tccatgccgg gctcgccgtg
    21241 gtccaggtag cgccattcgt cgaacaggtt ggggccgaag ccggtcttcc ggatcgattt
    21301 caggaactgc ttgggaatga tggcgtcggt gtcgacgttc tcgcggtcca gcggggcgac
    21361 caggcccttg tgggtggtga aagcgtccat gtcgtgggtt cgaggaaagg gttgctgggg
    21421 cgcgggcagc ggcggcgccc gatgatggac gggtggaatg cttcgcccaa gccaccgctg
    21481 gcgcacgcac ggggcgtgcg cctggggtgg tcgggcgaat ggcagcctgg ccatcgcctg
    21541 ccgggggcgt gtggccagtg cgggcgggcc gaacgtgggc ttacttcttg ccggcgttct
    21601 cgaccgccga gccggccttc tgcacgtcct tgccgacgcc ttgcacggta ttgcaaccgg
    21661 ccagcatcag ggctgcggca accagggcga gtactttttt catgatgagc tccttctggg
    21721 ggttaagaga tctggcgaat gtcgacgaaa tggccatgca cggccgctgc ggcggccatg
    21781 gccgggctga ccaggtgggt gcgcccgccc tggccctgac ggccttcgaa attgcggttg
    21841 ctggtggagg cgcagcgctc gcccggctcc aggcggtcgg cgttcatggc cagacacatg
    21901 ctgcagccgg gctcgcgcca ctcgaagccg gcggcggtga agatcttgtc caggccctcg
    21961 gcctcggcct gctgcttgac cagccccgag ccgggcacca ccatggccag cttcacgttc
    22021 ggcgccagcc ggcgacccag gcgcttgacc accactgcgg cggcgcgcag gtcttcgatg
    22081 cggctgttgg tgcagctgcc gatgaacacc ttgtcgacgt ggatgtcggc gatcggcttg
    22141 ccgggaatca gtcccatgta ggccagggcg cgttccatgg cgccgcgctg caccgggtct
    22201 ttctccttgt ccgggtcggg caccctggcg tcgacaccga ccaccatttc ggggttggtg
    22261 ccccaggtga cctggggcac gatggcttgg gcgtcgagtt cgaccacggt gtcgaaatgg
    22321 gcgccgtcat cgctgcgcag gctgcgccag ctggcgaccg cctggtccca ctcggggcca
    22381 ccgacgaagc ggccggtgac ggggtcggtg ccgggggcca tggggcggcc cttgacgtag
    22441 tcgatggtct tgtcgtccac cgcgatcagg ccggcgcgcg caccggcttc gatggccatg
    22501 ttgcacaccg tcatacggcc ttccatgctc aggtcgcgga tcgcctggcc ggcgaattcg
    22561 atggtgtggc cggtgccgcc ggcggtgccg atcctgccga tgatggccag cacgatgtcc
    22621 ttggcggtga tgccgggtgc caccgcgccg ttcacgcgca ccagcatgtt cctggccttc
    22681 ttggcgatca gcgtctgcgt ggccagcacg tgctcgactt cgctggtgcc gatgccgtgc
    22741 gccagcgcgc cgaaggcgcc gtgggtgctg gtgtggctgt cgccgcagac caccgtcatg
    22801 ccgggcaggg tggcgccgtt ttccgggccg atcacgtgca cgatgccctg gcgctggttc
    22861 atgaacggaa agtaggcggc ggcgccgtgg gcctgcatgt tggcgtccag cgtgacgatc
    22921 tgctccttgc tgatcgggtc ctcgatgccg tcgtagccac gctcccagcc ggtggtgggg
    22981 gtgttgtggt cggccgtggc gaccacgctg ctggtgcgcc agaccttgcg cccggcctga
    23041 cgcaggccct cgaaggcctg cgggctggtg acttcgtgca ccaggtggcg gtcgatgtag
    23101 agcagggcgg tgccgtcctc ttcggtgcgg acgacgtgct cgtcccagat cttgtcgtac
    23161 agcgttcgcg gggtcatgtc ggccattctc gtgattgtca aattctatgc tgcggctgcg
    23221 cgtcgtggga gcagcgcagg tgggctgcca gcctggggca ggtgccgatg ccgggccgcg
    23281 ccgtcatggt cggccgtgtg cggggtgcga ggcagttcga atccatgtag tccgaatggc
    23341 ggttggcgct tgtgtggcaa gcgccaacag ctatcgtttt ggtagtattg tggcggcggc
    23401 catgaaaaac ccgccacgcg ggcgggtgct cggcgcgcgg cgcggcgcgg gctcagggca
    23461 cgtcgcggcg cggcgagccg gtgaagagct ggcgcgggcg gccgatcttg tactcggggt
    23521 cggccagcat ttcgttgagc tgggcgatcc agcccaccgt gcgggccagc gcgaagatgc
    23581 cggtgaacag gctgacgggg atgccgatgg cgcgctgcac gatgccggag tagaagtcca
    23641 cgttcgggta gagcttgcgc tgcacgaagt agtcgtcctc gagggcgatc ttctcgacct
    23701 gcttggccag ggcgaacagc gggtcctttt ccaggcccag ctcggccagc acctcgttgc
    23761 aggtttcctg catgagcttg gcgcgcgggt cgtagttctt gtagacgcgg tggccaaagc
    23821 ccatgagctt gacgccgctg ttcttgtcct tgacctgctc catgaattca cccaccttgg
    23881 cgatgccgcc attgcgctgg atgtcctcga gcatgttcag gcaggcctcg ttggcgccgc
    23941 cgtgggcggg gccccacagg caggccacgc cggcggcaat ggcggcaaac ggattcgtgc
    24001 ccgaggagcc gcacaggcgc accgtggagg tggaggcgtt ctgctcgtgg tcggcgtgca
    24061 ggatgaagat gcggtccatc gccttttcca gcaccgggtt gaccttgtag tcctcgcagg
    24121 gcgtgccgaa catcatgcgc aggaagttgc cggcgtagga cagctcgttg cgcgggtaca
    24181 tgtagggctg accgacgccg tacttgtagg ccatggccac cagcgtgggc agcttggaga
    24241 tcaggcggat ggccgagatg tggcggtgct ccgggttatg gatgtcggtg ctgtcgtggt
    24301 agaaggccga cagcgccccg atcaggccgg tcagcacggc catcgggtgg gcatcgcggc
    24361 ggaaaccgcg caggaagaac tgcatctgct cattcaccat ggtgtgcttg agcaccagct
    24421 tgtggaaatc gctgcgctgg gtggggttgg gcagttcgcc gttgagcagc aggtagcagg
    24481 tgtccaggta gtcgcacttc tgtgccagct gctcgatggg gtagccgcgg tacagcagtt
    24541 cgcccttgtc gccgtcgatg taggtgatgg cggactggca ggatgcggtg gacagaaaac
    24601 ccgggtcgta ggtgaacatg cccgtctggc cgtacagctt gcggatgtcg atgacgtccg
    24661 gaccgatgct gccctggtag accggcagtt cgatgtcggg gctgccgttg gagaaggaca
    24721 gcttggcttt gttgtcggag agtttcatgc tcggattcct tgaatggagg cagacgttga
    24781 agcggagcga aaggcgcgtc aggcggcagc aggacgcagc agggtcagca actgacggat
    24841 gtcgggcgtg tcgaggtcgc catcgggttc cttgcggcgc agcagcaggt cgagcagctc
    24901 gttgtcggac aggtccatca actgggtcag tgcggcggcc tggcttgccg tcagctgctc
    24961 ggcgtggcgc tggaagaagc gctcgatcag cagatcgttc tccagcatgc cgcggcggca
    25021 gcgccacctg agcatgccga gggctttctc gtcgatgcgt tcgctctcca tggttgcgcc
    25081 ggcggatcgc agggtcgtca gatgccccgg cgaaccagga gctccttgat cttgctgatg
    25141 gccgcggccg gtttgaggcc cttgggacac acatccacgc agttcatgat ggtgtggcag
    25201 cggaacagtc ggtaggggtc atccaggttg tccagccgtt cgccggtggc ctggtcgcgg
    25261 ctgtcggcga tgaaacggta ggcctgcagc aggccggccg ggccgacgaa cttgtccggg
    25321 ttccaccaga agctcgggca gctggtggag cagctggcgc acagaatgca ctcgtacagg
    25381 ccgttgagct cctcgcgttc ctcgggcgac tgcaggcgtt ccttggaggg cgaggtgggc
    25441 tggtcgttct gcaggtaggg cttgatcgag tggtactggt tgaagaactg cgtcatgtcc
    25501 acgatcaagt cgcggatcac gggtagcccg ggcagcggct tgagcacgat gtcgcccttg
    25561 agcgtgttca tgttcgtcag gcaggccaga ccgttcttgc cattgatgtt catggcgtcc
    25621 gagccgcaca cgccctcgcg gcaggagcgg cggaacgaca gcgaagggtc ctgtgccttg
    25681 agcttgagca gggcgtccag cagcatgcgc tcgtggccgt cgagctcgac ctgcacggtc
    25741 tgcatgtaag gcttggcgtc cttgtccggg tcgtagcggt aaatcttgaa tgtgcgttgc
    25801 atggtgtgtg tctttcgcgt ggcagcgctc gggctcagaa cgtgcggacc ttgggcggga
    25861 tgctgtcgac cgtcagcggt ttcatgttca ccggcttgta gctcaggctg ttggtggcgc
    25921 tgtcccacag cgtgtgcttg agccagttgg cgtcgtcgcg gcccagcggg gcggtggggt
    25981 ggtccgccgg gtgttcgtag tcgtacaccg tgtgggcgcc gcggcattcg tgacgcgcgg
    26041 cggccgagac catggtggcc tgcgccacct cgatcaggtt gtcgacctcg agcgcttcca
    26101 tgcgggcggt gttccagacc ctggacttgt ccttgagcgt gacctgtccg acccgctcgc
    26161 ggatgccagc gatcttgacc acgccttcgt ccatgccctt ctgcgtgcgg aacacgccgg
    26221 catgctgctg catggtctgg cggatttcgt tcgccagatc ctgcgcgtag atgccgtcgg
    26281 tggagttttc gagctggttc aggcgcgcca gcgtgcgctc gctggcgtcg gccggtaccg
    26341 ccgcctgttc gccgtagctt ctgacgaact gcacgatgtg tttgccggcc gacttgccga
    26401 acaccagcag gtccaagagc gagttggtgc ccaggcggtt ggcaccgtgc accgacaccg
    26461 cggcacattc gcccacggcg tacaggccgt tgacgatgtt gttcttctcg ccgtcccagg
    26521 tcacgacctg gccgtggatg ttggtcggga tgccgcccat ctggtagtgg atggtgggca
    26581 cgacggggat gggctccttg gtgatgtcca cgttggcgaa gttgtggccg atctcctcca
    26641 ccgagggcag gcgcttcctg atggtgtccg cgcccaggtg gtcgagcttc atcaggatgt
    26701 agtccttgtt cggaccgcag ccacggcctt ccttgatctc ctggtccatc gagcgggaga
    26761 cgaagtcgcg cggcgccagg tccttcaggg tgggcgcgta gcgctccatg aagcgttcgc
    26821 cgttgctgtt gagcaggatg gcgccctcgc cgcggcagcc ttcggtcagc agcacgcccg
    26881 cgccggccac gccggtggga tggaactgcc agaactccag gtcctgcagc ggaatgccgg
    26941 cgcgcgccgc catgcccaga ccgtcgccgg tgttgatgaa ggcgttggtc gacgcggcaa
    27001 agatgcggcc cgcgccgccc gtggccagca gcacggcctt ggcgtgcagc tcgtacagat
    27061 cgccggtctc gagttccagt gcggttacgc ccacgacgtc gccggcgctg ttgcggatca
    27121 ggtccagagc catccactcg acgaagaagt tggtcttgga ctcgacgttc ttctggtaca
    27181 gcgtgtgcag catggcatgg ccggtacggt cggccgcggc gcaggcgcgc tgcacgggct
    27241 tttcgccgta gttggccgtg tggccgccga aggggcgctg gtagatggtg ccgtcggggt
    27301 tgcggtcgaa cggcatgccg aaatgttcga gctcgatcac gacgttgggt gcctcgcggc
    27361 acatgaactc cacggcgtcc tggtcggaca gccagtcgcc acccttgatg gtgtcgtaga
    27421 agtggtagtg ccagttgtcc tcgctcatgt tgcccagcga cgccgacacg ccgccctggg
    27481 cggccacggt gtgcgagcgg gtcgggaaga ctttggacag gcaggcgacg ttcaggccgg
    27541 cgcgcgcgag ctcgagcgag gcgcgcatgc cggagccgcc ggcgccgacg atcaccacgt
    27601 cgaacttgcg ggtgatgact tggtctttgg tgtaactcat ggtcgtggtt cagtgatgct
    27661 tgtcatcggg tacggaacgc ggcgctcaca gacgccacag cgcctgcagg gcccagccgc
    27721 cgcaactcac cagccagatg acggtgaacg tctgcagcag caggcgcacg ccggcggcct
    27781 tgacgtagtc cagccagatg ctgttcatgc cgatccaggc gtgccagatc agtgcgacga
    27841 tgacggagaa ggtcaaggcc ttcatccact gggcggcaaa gatgccggcc caggtgtcgt
    27901 agccgatggg gccctgggtg aacagcacct gcagcagcac gatcagcgtg aaggccgcga
    27961 tcagcgcgcc gctgatgcgc tgcaccagga aatcgcgcca gccgtaatgg gcgccgacca
    28021 ccagtcgctt gttgccgtaa gtcgtggaca tgttgttgct cctggcaggt cagatcaata
    28081 caggccaaac agcttggccc cgagggccag cgtgaggacg atgctcacca ccagcgtggc
    28141 gacggccgac tgacggccga actccttggt ggtgcgatgc gagatgtcga ggtacaggtg
    28201 acgcacgccc gccagcaggt gatgcaggta ggcccagatc agcgccagca ccaccagctt
    28261 gacgaaccag gccggaaacg cccccaggcc ggacgagaag gcggcgctga actggccgaa
    28321 cgaaatctcc gacgacacgg atttgtcgaa cagccagatg atgaagggca gcagcagaaa
    28381 catcagcgcc ccgctggccc ggtgcaggat ggacacccag ccggccaggg gcaaccggta
    28441 ggtgcgcacg tcgttgaaga tgttgatgtt gcggaattcg ggccgcggct tggtggcttc
    28501 gctcatggga actctcgtgg gaagggattg cagagctgca attcttggag ggggagtgca
    28561 aattctattg caatgcagca agctgacggg acgattgcag gggagggtcg gcaggtcgat
    28621 cgagcaggag catgagccgc gtcaactcag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28741 nnnnnnnnnn gcgtcaactc agtgtcaact cagtgcattg cggtagtggt gggtgtcggt
    28801 gcggtacagg ccgcggcgca gttccatcgg ctcgtcgttg taggtgtagg caatgcgttc
    28861 gacgctcagc aacggggtgg cgggcgtgac ctccagcagc gcggcctgcg tcgggtcggg
    28921 cagcaccgcg cgcagctttt cgtcggcgcg caccatgcgc acgccgaact cggcttcgaa
    28981 cagggcgtac atcgggccgt ggtactgcga caggcgctcg gccgtcagtc ccttgaacgg
    29041 accggctggc agccacagat cttcgaggat ggtcggcaca ccggcaaagc gcagcaggcg
    29101 gcgcgcctgc agcaccggat cgcccgcgcg cagaccgagc aggcgcgcca cgtcggcgct
    29161 ggcgcgctgt cgcttgcagt cgacgaagtc gcgctgggcc ggcccttcgc tgtcggccgt
    29221 gccgcggtcc ggttgcaggc gcagaaagcg gtactgcacc tgttgctcgg cgtgggtggc
    29281 cacaaaggtg ccacgcccct ggcggcgcac cagaaggttg tcggccgcca gctcgtccac
    29341 cgccttgcgc accgtgccct ggctgacctt gtagcgcgcg gccagatcga actcgctcgg
    29401 gatcacctcg ccgggcttcc attcgccctg ttgcaggctc tgcaggatca ggctcttgat
    29461 ctgctgatac agagggctga acgccggcgc ctgcccaccc caggcggtgg caggtgcaag
    29521 cgccggcggg gcgctttcgg tcggggtgga agagggtgct gcgtgggcca tgtcgggtgt
    29581 cagtctgacg taggactgga tggcgatgat atcttatata agacacaaga caaattgacg
    29641 aatcagggtt ttcgcggata cacttggacg gtcgatttct cgtcctgcag gggtgccagc
    29701 cgtggctgcc cgcgccctgt ggtcagccac ttttcaccac aaccttctgg agttttccca
    29761 tgagcaagaa gcccgttcgc gtcgccgtca ccggcgctgc tggtcagatc ggctacgccc
    29821 tgctgtttcg catcgcctcg ggcgaaatgc tgggcaagga ccagccggtg atcctgcaac
    29881 tgctggagat cccggacgag aaggcgcaga aggcgctcaa gggcgtgatg atggaactgg
    29941 aagactgcgc cttcccgctg ctggccggca tgcaagcgca cagcgacccg atgaccgcct
    30001 tcaaggacac cgactacgcg ctgctggtgg gtgcgcgtcc gcgcggcccc ggcatggagc
    30061 gcgccgacct gctggccgcc aacgcccaga tcttcaccgc ccagggcaag gcgctgaacg
    30121 ccgtggccag ccgcaacgtc aaggtgctgg tggtgggcaa cccggccaat accaacgcct
    30181 acatcgccat gaagagcgcg cccgacctgc cggccaggaa cttcaccgcc atgctgcgcc
    30241 tggaccacaa ccgcgcggcc agccagatcg ctgccaagac cggcaaggcc gtcaaggaca
    30301 tcaagaagct cgccgtgtgg ggcaaccact cgcccaccat gtacgccgat taccgcttcg
    30361 ccaccatcga tggcgccagc gtgaaggaca tgatcaacga ccaggaatgg aacgccaacg
    30421 tgttcctgcc cacggtgggc aagcgtggcg ccgccatcat cgacgcacgc ggcctgtcgt
    30481 cggccgccag tgccgccaac gccgccatcg accacatgcg tgactgggcg ctgggcacgg
    30541 gcggcgaatg ggtgaccatg ggcatcccgt cgcagggctg gtatggcatc cccaaggagg
    30601 tgatgttcgg ctttcccgtc acctgcgaga acggcgaata caaggtggtc gaagggctgc
    30661 cgatcgatgc cttcagccag cagtgcatcg acaagacgct ggccgagctc gaaggcgaga
    30721 aggacggcgt caagcacctg ctgtgaggat gagggctgct gtcgccgaac cgatggggtt
    30781 tcgttgtgaa agaccttgga ggccatgtct ggcaagcgcc agcagctctt gttttgatag
    30841 tttgccgatg ccgttgcggc gccgcgctcg cgcagcttga caccaccgcc tgatccatga
    30901 ctgccgtcgc gcgccatccc cgacaggtgc tgctgggtgc ccaggccggt gccgtggtgc
    30961 tgccggtgtg tgaccactac agcggcgtcg aagcgcgcat gcgcaagagc ctgcagctgc
    31021 aggccgagat gacgcaggag ttcggcacct gcgtgttcga cgtcacgctg gactgcgagg
    31081 acggcgcccc ggtgggtggc gaagccgagc acgcccagtg cgtgaccgaa ctggcactgg
    31141 ccgccgcgcc cgacgcacgc gtgggcgtgc gcgtgcatcc ggtcgatcac ccggcgttcg
    31201 aagccgacat ggcgcagatc gcgggcaagg ccgggcaccg gctggcctac atcatgatcc
    31261 ccaaggtgga gtcggtggag gatgtggtgc acgccgagcg cgcgctgcgg gccagctttg
    31321 cgccgggcct gccgttgcag gtgctgatcg agtcgccggc ggcggtgcac cgtgccttcg
    31381 acatcgccgc gcacccgggc gtgcagtcgc tcagcttcgg tctgatggat ttcgtctcgg
    31441 cgcacgccgg cgccatcccg gccgaaggca tgggcatcga ggggcagttc acgcacccgc
    31501 tggtgctgcg cgccaagctc gaaatcgcat cggcggccca cgcgttcggc aaggtgccct
    31561 cgcactgtgt ggtgaccgag ttcagcgacg tgcaggccat gcgcgtggcc gcgcggcgcg
    31621 cggcgcgcga gttcggctac gcgcgcatgt ggagcatcca ccccagtcag atccggccca
    31681 ttctggaggc ctttgcgccc gacgaccggc agatcgacct tgccagcgcc atcctgctgg
    31741 ccgcgcagca ggccgactgg gcacccatca gccaccaggg tgccttgcac gatcgcgcca
    31801 gctaccgcca tttctggcag gtgctggagc gtgctcacgc caccgggcgc cacttgcccg
    31861 gcgatgtggc ccactggttc tgaccccatg ccatgtcatg atgagtggtt gttgaaacat
    31921 tcctgaaagc gagttccaca tgaagccttt tgccatgacc agcctggcca tcgttcttgg
    31981 cctttccatg ggtgcggtcc aggttgcgtc ggcggccgac caaccggcgc cggccaagaa
    32041 ggtcacgacc caggccaaga aagcgccgac caagaaggtg ccggtcaagc aggccgccac
    32101 cagcaagggt gtcccggcca agacggccaa ggcggtcgag gccaacaccc ccgtgcagac
    32161 gctgagccag cgcctgaccg acaccgaact ggagatcgcc aaaaccattc agaccggtcg
    32221 catccagtgc gagcttggtg ccgatgtgac ggtgtccgcc gacgagaaga accccggttt
    32281 cttccacgtg gtggcgggca agcagcgcta ctacatgcac ccggtggaaa gccgcaccgg
    32341 cgccgttcgc atggaagaca accgcgccgg cgccatgtgg ctgcagctgg gcaacaagtc
    32401 catgctgatg aaccagaagc tgggccagcg cgtcgccgac gagtgcgtga cgccggcgca
    32461 gcgcgagttt gcgttgcaga tgaagtccag caatcaaccc ggtctgctgg acggacagac
    32521 caaatagatt ttctgccggc ggctgccggt ttggcctcgt gcgcaggtgc ggggcgagcc
    32581 ggctcgcgcg cgggtacctc acgcgtccca tcgatatcac ccagtcagga gaacacagca
    32641 tgctgcaagc ctaccgagcc cacgccgccg agcgcgccaa actgggcatc ccgcccctgg
    32701 cgcttgatgc caagcaggtc gccgagctga tcgagctcat caagaacccg ccggccggcg
    32761 aagagcaatt cctgatggat ctgctcacgc accgcgtgcc cccgggcgtg gacgacgccg
    32821 ccaaggtcaa ggccagcttt ctggccgccg tcgcgcatgg cgacatccag gtgggcctga
    32881 tctccaaggc caaggccacc gagctgctgg gcaccatggt gggcggctac aacgtgcacc
    32941 cgctgatcga gctgctcgac gacgccgaag tggcccaggt ggccgcggac gcgctcaaga
    33001 agacgctgct catgttcgac tacttcaacg acgtggccac caaggccaag gccggtaacg
    33061 ccaaggccaa ggaagtcatg cagagctggg ccgacgccga gtggttcacg agccgtccca
    33121 aggtggccga gaagatcacc gtcaccgtgt tcaaggtgcc cggcgagacc aacaccgacg
    33181 acctctcgcc cgcgcccgac gccacgacgc gccccgacat ccccatgcac tacctggcga
    33241 tgctcaagaa cacgcgcccc gacgcggcct tcaagcccga gcaggacggc gtgcgcggcc
    33301 ccatccagtt catcgaggac ctgaagaaaa agggccatat cgtcgcctac gtgggtgacg
    33361 tggtgggtac gggctcgagc cgcaagtcgg ccaccaacag cgtgatctgg gccac
//
LOCUS       DAOQTZ010000015        32960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1558_length_32960_cov_14.281203, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000015 DAOQTZ010000000
VERSION     DAOQTZ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 32960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32960
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1558_length_32960_cov_14.281203"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PKN51_03285"
     CDS             complement(<1..1194)
                     /locus_tag="PKN51_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT84153.1"
                     /translation="MEFIAANFAPVMFAGLIVFLILGFPVAFSLGAAGLFFALVGVEL
                     GVFTSAIYSWLPQRLIGIMSNETLLAVPFFTLMGLILERSGMAEDLLETVGQVFGPLR
                     GGLALAVVLVGAMLAATTGVVAASVISMGLISMPIMLRYGYDRRLASGVIAASGTLAQ
                     IIPPSLVLIVLADQMGKSVGDMYKGAFIPGFALMGLYILWVFALAIFKPSMVPALPPE
                     ARTFREPNGHSGYRSLGVLALLSTAVAVVLARNMEAVHTWWQGTPVEFVPTDEKIVVA
                     MCGGTFVAWLIALVNRITRMGLLSQLAERVTFVLIPPLLLIFLVLGTIFLGVATPTEG
                     GAMGAMGALIMAAMRRRINMDLLKQALGTTTKLSSFVMFILIGATIFSMVFQAADGPV
                     WVEHLM"
     gene            complement(1218..1814)
                     /locus_tag="PKN51_03290"
     CDS             complement(1218..1814)
                     /locus_tag="PKN51_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNT84154.1"
                     /translation="MQALLKFSRAVDWLNASLGRFVIWLIFGATIISALNAVVRKAFD
                     TSSNAFLEVQWYLFAWSFLAAAGYTLLHREHVRIDVLNSRLPKNVQLWIEILGYVLFL
                     TPVCVIVLWMSTPIVFERIASGEMSPNPGGLIRWPVWAALPIGFVLLLLQGWSELIKC
                     IAFLRGQGPDPTVKALDKTAEQELADALRQQQSASQTH"
     gene            1970..2710
                     /locus_tag="PKN51_03295"
     CDS             1970..2710
                     /locus_tag="PKN51_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT84155.1"
                     /translation="MSTLAESARAALQLIASLDPLLWSVVGRSLAVSGLACLLACGVG
                     LLAGASLAVARFRGREAVLTLLHTLLALPSVVVGLLVYLLLSRSGPLGFLGWLFSFQA
                     MVLAQAVLVVPVIAALTRQTVEDADLRHGEQLASLGAGPCLRGLLLAWDERCALLMVL
                     MAAFGRAVSEVGAVMIVGGNIDGFTRVMTTAIALETSKGDLPLALGLGMVLLSVVLLL
                     NAAMAGLRHWRARTDALAPGAVQGRWAA"
     gene            2707..3450
                     /locus_tag="PKN51_03300"
     CDS             2707..3450
                     /locus_tag="PKN51_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT84156.1"
                     /translation="MSRPAGPPGAAAPLIRLQDVGVRFGRVQALQGVGLEIAAGECVA
                     LIGGNGSGKSTLLRVIHGLIAPTEGQLVVPARRAQAMLFQRPWMMRTTVLRNVALGLW
                     LRGLPWRQAQERALRALERVQLAELASRRAPGLSGGQQQRVALARAWAQQAQLLLLDE
                     PTASLDPHARLEVEALMRAFTGTAPDAAPLTMVFASHHLGQVRRLAQRVICLDQGRVL
                     ADLPVAQFFDHARLSSVAPVAAAFVQGEM"
     gene            3450..4277
                     /locus_tag="PKN51_03305"
     CDS             3450..4277
                     /locus_tag="PKN51_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT84157.1"
                     /translation="MQKFRNFWPLALLQTALAAILTIAFPVAWAQTIVMASTTSTEQS
                     GLFAHLLPQFRQASGIDVKVVAVGTGQALDIARRGDADVLFVHDPKAEEQFVTDGWGL
                     KRHPVMYNDFVLVGPRSDPAGARGRDIVQAMRQLAGAHAPFISRGDKSGTHSAELRYW
                     AQAGLTEARGRGYRECGCGMGPALNMAAASGAYVLADRGTWLNFRNRADLAILVEGDP
                     RLFNPYGVLVVNPARHPQVKAAEAQKFVDWVTSPAGQAAIASYRIGGQALFFPDARP"
     gene            4406..5563
                     /locus_tag="PKN51_03310"
     CDS             4406..5563
                     /locus_tag="PKN51_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNT84158.1"
                     /translation="MGVALGLLLLPAQAQIRVSINPGDQIEADRVGIFNAWKGALDAG
                     LRKAGASAAQARFSSDATADMNAGRANVFDLMVGPAPVIGSAVRYGYVPVVGATQKAR
                     AVLVAAEGSAIADWAQSKGKRLGMPGQDSVVTYLLRGEVQAGNTTLARQYPSIYQTRY
                     QDALLTCLELRRCDVVAVEEVVAKRWAASGKKVKVVWQSREVPGLSVALHKDAQVSAD
                     TLRSALMAELARLDAFGYTGTLTVKDFEYVSTLGYFTPRTLEGATLIEDPAQVESLLA
                     AGARYIDARNQEEFAAGHVPGAILVPYVEKSAKDPGYDPKLDQFDLQRLGDNKAQPLV
                     FGCNGPECWKSFKASRAALQAGYTKVYWFRTGVPAWRSSGRPLDRGDAKAG"
     gene            complement(5572..6678)
                     /gene="modC"
                     /locus_tag="PKN51_03315"
     CDS             complement(5572..6678)
                     /gene="modC"
                     /locus_tag="PKN51_03315"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HNT84159.1"
                     /translation="MTQGTGHDIELRAQVRMGSGFRLGVELRLPGQGVTALFGPSGCG
                     KTTLLRAVAGLTRPSPGRVAVGGDVWQDDAHRIWRPTHRRPLGLVFQEASLFEHLTVQ
                     GNLDFGLQRVPAAERRVALPQAIELLGLGPMLARRPAQLSGGERQRVAIARALATSPR
                     LLLMDEPLASLDAARKAELLPYFERLTRELRLPMLYVTHALDEVIRLADHLVLLRDGE
                     VVAHGPVEALLTRPDVARTQGDAASALVSAQVLSFDPHYQLLQVAFDHGVLHCVHSAG
                     TPPRTPGQRVRLRVQARDVSLCLSAAHDTSILNVLPARVRSLAEDGPAQVLVALDLGA
                     TPLLARVTRKSAEALALAPGQSVYAQIKGVAVLD"
     gene            complement(6675..7361)
                     /gene="modB"
                     /locus_tag="PKN51_03320"
     CDS             complement(6675..7361)
                     /gene="modB"
                     /locus_tag="PKN51_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNT84160.1"
                     /translation="MLDATSLAAVRLSIELALATTCILLVLGTPLAWWLARGDAGALA
                     WLRTPVRTLVAMPLVLPPSVLGFYLLVMLGPQGPLGRLTQALGWGTLAFSFWGLLLGS
                     LVYSLPFVVQPLVNAFEAIGRRPLEAAATLRASPLDAFLHVALPLARPGYLTGAVMGF
                     AHTLGEFGVVLMIGGNIPGQTRVISVQIYDHVEALEYTQAHWLAGGMLVFSFLVLLTL
                     HLTQSRKAPA"
     gene            complement(7471..8226)
                     /gene="modA"
                     /locus_tag="PKN51_03325"
     CDS             complement(7471..8226)
                     /gene="modA"
                     /locus_tag="PKN51_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT84161.1"
                     /translation="MRPIRTLVTLAAVMVASWSAHAETVHVAVAANFTEPAKALAAIL
                     EKTTGHEARLSFGASGAFYTQIKNGAPFDVFLSADDERPARLEKEGDTVPGSRFTYAT
                     GQLVLWSAKPGLVDDKGEVLQGGQFNKLAIANPKNAPYGAAAVEAMNRLGLQATLQPK
                     LVTGESIGQTYNFVATGNADLGFVAMSQVLQGGRLKGGSMWVVPGSLHAPIVQDAVIL
                     KRGQGNPAAKAWMALLSSPRTKDLIRSYGYAVK"
     gene            complement(8298..9467)
                     /locus_tag="PKN51_03330"
     CDS             complement(8298..9467)
                     /locus_tag="PKN51_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme enzyme"
                     /protein_id="HNT84162.1"
                     /translation="MTHTPLRARVAAALALLSLAACGGGDSADAGPPPVPPAWAWSLP
                     AGLAPPLVPADNPMNAAKVELGRHLFYDVRLSVNNTLSCAGCHAVNLAFADGRAVARG
                     ATGQNHPRNAPSLVNAAYQATLDWSNPTPRTLEQQMHTPLFGEQPVEMGVNDTNRDAI
                     VQRLKDDARYPPRFAQAFGGEAMPIHWDNVIKAIAAFQRTLVAAGSRYDQALAGTATL
                     TESEQRGQRLFFGERALCAQCHSGPNLGGGAFVSAQGRVGEPGFYNIGLFNIGGTGAY
                     PTNNRGLLEFTGRPEDMGRFRAPSLRNVALTAPYLHDGSIATLEAVVALHAAGGRVLG
                     NGPYEGDGRANPYKDPRIDQIDIDAQDQADLVAFLRALTDDRMLARPEFADPFVP"
     gene            9556..10530
                     /locus_tag="PKN51_03335"
     CDS             9556..10530
                     /locus_tag="PKN51_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84163.1"
                     /translation="MNMTARQDTDSFWARARRGLLVATGFALLSLAACGGNGDDEVAD
                     RGPTTLEVQAKLNGLYWDAQASRLYLTDDEASVNAIRIWDGDDKFSVAHQLPAMEANQ
                     RHSLGQLTRAADGKLYVTRFGFGSYGTVVAVPKSGQPYNLTGLAGDRRRIAITPTADG
                     KLIDGWFRGGGAPGPSGVISELTVAAGGQASEQDLITGLSKPAGVAVVGDQLFVSDQG
                     TGKVLAYSLAAVRAQPAKAEDGRVVAEFISADSIDLMTAGPDGTLFFGGGAGTLYAVA
                     PDGKVRTLASGWPGIRGVAYDAQNKRLFAAVTAPNAASPATIRIVPID"
     gene            10830..11579
                     /locus_tag="PKN51_03340"
     CDS             10830..11579
                     /locus_tag="PKN51_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNT84164.1"
                     /translation="MTKKTLSASSLGYGQADKRILILQLVGAHGSISQAARVAGVSYK
                     AAWQALHTLTNLAGEALVDSSVGGAGGGGARLTPAGERLLHAATRLAQARQAVLSQLG
                     AADDAIAAPRTSMRNHLRARVLELVGAGPREPMVRVRLALDGGAELAALITRESAELL
                     GLAPGLALLALTKATAVRVVPAASGAPRPGVLPGRVLRVFRGTHGDEVTLTLTGGQQL
                     VGFAERPNRLRAGSAALAQVDDRVVVLALPG"
     gene            complement(11611..11997)
                     /gene="crcB"
                     /locus_tag="PKN51_03345"
     CDS             complement(11611..11997)
                     /gene="crcB"
                     /locus_tag="PKN51_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNT84165.1"
                     /translation="MHAVLAICLGACAGALLRWQLGLWLNHAGAWLPWGTLAANLIGG
                     YLVGLAVALLHHHPQLDPTWRLLLVTGFLGALTTFSSFSAEVVDMALAQRWGLAAVTV
                     LAHLSGSLLLTFAGIRTALVLIAARA"
     gene            complement(12033..13031)
                     /locus_tag="PKN51_03350"
     CDS             complement(12033..13031)
                     /locus_tag="PKN51_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNT84166.1"
                     /translation="MFKNLMLYRLGPGWPASAAQLEKALAREPFAECGATQQKSTGWV
                     APRGEQHGALVEAVDGQWIARFAIETKPVPGDAVRRKVQQAVAEIEKTSGRKPGKKEL
                     RDLRDDALLALLPQAFARRSQVTLWLNPQQRWLALDAGSQGKADEVIASLVRVAGQGF
                     AIHLLQTAHSPQAAMTGWLAATSADDWPEGLHVERECELKGSGDEPAVVKFTRHELAT
                     DEVRQHIAEGKLPTRLALGWQGRVGFVLTQALQLKKIGFLDGVFDERPAPASADERFD
                     ADVALATGELRGLITDLIAALGGEIEPGTPSGFTEAPTPASTVTEEATVAADDPPF"
     gene            complement(13325..14620)
                     /locus_tag="PKN51_03355"
     CDS             complement(13325..14620)
                     /locus_tag="PKN51_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT84167.1"
                     /translation="MSDFAITGLLILSLFALLGSGVWIGLTLTGVAWIGMQLFSARPA
                     GDAMAVTIWGSASSWTLTALPLFIWMGEILFRTKLSESMFRGLAPWVTWLPGRLLHTN
                     VIGCAIFAAVSGSSAATCATIGKMTLPELGSRGYPRDITIGSLAGAGTLGLLIPPSII
                     MIVYGVTADVSIAKLFIAGILPGILLAALFSGYLMVWALLHPDQIPMPDRRLSLAEKL
                     HESRHLIPVVLLIAAVLGSIYSGVATATEAAAVGVIGSLLLSLVQGSLSWSTFKTALL
                     GATRLYCMIALILAGAAFLTLAMGYIGLPRHLAEFIGGLGLSPFMLVLALAVFYIVLG
                     CFLDGISMVVLTMGVILPTVQAAGLDLIWFGIFIVIVVEMAQITPPVGFNLFVLQGMT
                     RHDIAYIARVTLPFFFLMCAMVLLLWFFPQIATWLPKNM"
     gene            complement(14613..15125)
                     /locus_tag="PKN51_03360"
     CDS             complement(14613..15125)
                     /locus_tag="PKN51_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT84168.1"
                     /translation="MRRLLDFLYDAAAWLAALAMVGVLLMVLTSILGRLLDFHLPGTD
                     AYAGYSMAAAGFLSLAHTLKKNEHIRVTLLMGRLRGGARRGMELWALSVAVLLAGLFA
                     WFSVRLVWQSHLFNDMSTSNDATPLWIPQIAMAVGTVILLLAFIDEWVLELRGLRQHE
                     SGDEEALHNE"
     gene            complement(15217..16203)
                     /locus_tag="PKN51_03365"
     CDS             complement(15217..16203)
                     /locus_tag="PKN51_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT84169.1"
                     /translation="MTLTRRTLGALAVLSLGGFALSAAAQTKWDLPSAYPASNFHTEN
                     LVQFAKDVDAATGGKLKIQVHDNASLFKAPEIKRAVQGGQAQAGELLMVNFQNEWPVW
                     GLDGIPFLATSYAESKKLYQASKATVQKKLAEQGMVLLYAVAWPPQGIFTKKQVGSAA
                     DLKGIKWRAYSPATARIAELVGAQPVTVQQAELSQAMATGVVESYMSSGSTGYDTKTY
                     EHLKFFADTQAWLPKNAVIANKKAFDALDKATQDGLLKAAADAEARGWATSERKTNEY
                     LDLLRKNGMTVYAPSAQLMTDMRKVGDTMLKEWLDKAGAEGKAVVDAYRAAK"
     gene            16442..20131
                     /locus_tag="PKN51_03370"
     CDS             16442..20131
                     /locus_tag="PKN51_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNT84170.1"
                     /translation="MSEHALHATRWQFWIDRGGTFTDIVARRPDGTLVTHKLLSDNPE
                     QYPDAAVAGIRQLLGLASGVPITPAQVACVKMGTTVATNALLERKGEPTLLVTTRGFR
                     DALRIAYQARPRLFDRHIVLPELLYAAVVEASERVDADGAVVLPLDEVLLKQELLAQY
                     QSGLRSVAIVFIHGWKHVAHEQAAARLAREIGFTQVSTSHQTSPLMKFVSRGDTTVVD
                     AYLSPILRRYVDRVAAEMPGVPLYFMQSSGGLTDAHAFQGKDAILSGPAGGIVGMART
                     AQLAGQQRVIGFDMGGTSTDVSHFAGQFEREFETQVAGVRMRAPMMSIHTVAAGGGSI
                     LRFDGTRYRVGPESAGANPGPASYRRGGPLALTDANVMVGKLQPAYFPRVFGPAADAP
                     LDAEVVRARFAEIAAQDAGGGRTPEEVAHGFIQIANQQMASAIKKISVARGYDVTRYT
                     LQCFGGAGGQHACAVADALGMNRVLVHPLAGVLSAYGMGLADQTLMRERALELPLEIG
                     QLAALRAAGDALAADARAQLQGQHAGGGAILVHQRLHLRYAGTDSALVVPFDRSLDGV
                     GAAAALQAITEAFEAAYRQRFAFLMQGKALIVEAVSVEAVIAGEAPAEIEQPLQPPRS
                     VPRRDTVRIYTSGSDGQSGWHQAALIVREDLRAGDVIEGPAIIAERNATTVLEPGWRA
                     RLTGLDHLLLERAAPRAARFAAGTQVDPVLLEVFNNLFMNIAEQMGLQLQNTAYSVNI
                     KERLDFSCALFDAAGHLIANAPHMPVHLGSMGESIQTVIARNPDMQRGDVYVLNDPYH
                     GGTHLPDITVITPVYLDGEARPVFYVGSRGHHADIGGITPGSMPPFSTRIEEEGVQID
                     NVKLVDRGRLCEREVLELLASGEYPSRNPQQNLADLKAQIAANEKGVQELRKMVDQFG
                     LDVVQAYMQHVQDNAEESVRRAITRLAARVKDGHFTLPLDNGAQIQVAVAIDSATRSA
                     KIDFSGTSPQQSNNFNAPTAVCMAAVLYVFRILVDDDIPLNAGCLKPIEVTIPAGSML
                     RPEPPASVVAGNVETSTCITNALIGALGLMGASQCTLNNFTFGNERYQYYETVSGGSG
                     AGGEFDDQGRLVGGFDGTSVVQTHMSNSRLTDPEVLEWRFPVRLESYEIRHGSGGAGR
                     WRGGDGGVRRLRFLEPMTASILSNGRVHGAFGMAGGGAGAVGINRVLRADGSVQELQH
                     IGSVEMQAGDIFEIHTPGGGGYGAR"
     gene            complement(20390..20770)
                     /locus_tag="PKN51_03375"
     CDS             complement(20390..20770)
                     /locus_tag="PKN51_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNT84171.1"
                     /translation="MLKKILVLLSLLYAAFAFAAVDANTASAADLDSVKGIGPGMSSR
                     IIKERKKSEFKDWADFISRVKGVGEKNAAKFSAAGLTVNGAAYTAAAAASPAKPKAAP
                     AAASSNTAAKTPAPSAPATAASKP"
     gene            complement(20890..22050)
                     /gene="lapB"
                     /locus_tag="PKN51_03380"
     CDS             complement(20890..22050)
                     /gene="lapB"
                     /locus_tag="PKN51_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HNT84172.1"
                     /translation="MEFDLGWILFGLPVVFALGWFASRLDLRQLRIENRQAPKAYFKG
                     LNHLLNEQQDQAIDAFIEAVQKDPDTSELHFALGNLFRRRGEYERAVRVHEHLLSRAD
                     LSQADRDRAQHALALDFLKAGLLDRAEDALRKLEGTAFEEQAGLTLLSLHERARDWPQ
                     AGALARRLQQRGAGDFTLRLSHYQCEQASEALNKKSDRTAAQQYLRLAIDQAPHAARP
                     RIELAGLLAREGDAADALDQLASLEQHAPQAIALVASELVRIGQACGQATRVRELLQR
                     SYASTPSIDVADAIVQADVTAGVPQAQARSGYLRHMAQEPSLIAAARWLSHEGFAQQD
                     AHALVQRSVDHAARPLARYRCAACGFEAQGYFWQCPGCQAWESFPPRRIEEL"
     gene            complement(22040..22333)
                     /locus_tag="PKN51_03385"
     CDS             complement(22040..22333)
                     /locus_tag="PKN51_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNT84173.1"
                     /translation="MNRLARLLTWILKAAVFFALFAFALNNQQGATVHLLFGHQWRAP
                     MTLIVLVAFGIGLIVGVLGMLPGWWSRRHGRTPMPGPVPAPSEPSVPEPPHGV"
     gene            complement(22361..22693)
                     /locus_tag="PKN51_03390"
     CDS             complement(22361..22693)
                     /locus_tag="PKN51_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNT84174.1"
                     /translation="MTRSDLVELLAERFSQLTQRDADAAVKTILDAMSDALARGHRIE
                     IRGFGSFSINHRPPRLGRNPRTGDRVHIPERRVPHFKPGKALREAVGAQPVSPGVRGA
                     PEDRETPH"
     gene            complement(22741..24420)
                     /gene="rpsA"
                     /locus_tag="PKN51_03395"
     CDS             complement(22741..24420)
                     /gene="rpsA"
                     /locus_tag="PKN51_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNT84175.1"
                     /translation="MSESFAQLFEESLQRTEMRPGEVITAEVVRVEHNFVVVNAGLKS
                     EAYIPIEEFKNDQGEVEVEPGAFVPVAIGSIENGYGDTILSRDTAKRLASWLALEKAL
                     DSGEFVTGTTSGKVKGGLTVLVNGIRAFLPGSLVDTRPTKDLTPYENKTMEFKVIKLD
                     RKRNNVVLSRRAVVEASMGEERAKLMETLKEGAIVNGVVKNITEYGAFVDLGGIDGLL
                     HITDMAWRRVRHPSEVVQAGQEITAKILKFDTEKNRVSLGLKQMGDDPWLGVSRRYPQ
                     GTRMFGKVTNIADYGAFVELEPGIEGLVHVSEMDWTNKNVAPSKIVSMGDEVEVMVLE
                     IDEDKRRISLGMKQCRANPWQEFAQNTRRGDRVKGPIKSITDFGVFVGLAAGIDGLVH
                     LSDLSWNEPGEAAVRNYKKGQEVEAIVLAVDVDRERISLGIKQLDQDPFTTFVSVNDK
                     GQTVTGTVKTVDARGAEIDLGNDVVGYLRASEISRDRVEDASQVLKVGDEVTAVVINV
                     DRKSRNIQLSVKAKDAADQQEAMASLSQSSGSAGTTSLGALLRAKLDGNDK"
     gene            complement(24643..26649)
                     /locus_tag="PKN51_03400"
     CDS             complement(24643..26649)
                     /locus_tag="PKN51_03400"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HNT84176.1"
                     /translation="MFDLPFLDIPALRAVHGTVRLPGSKSISNRVLLLAALCEGRTVI
                     HDLLDSDDTRVMLQALRQLGCVVELQGTSHVIEGLGSRPRQPQAELFLGNAGTAMRPL
                     TAALAVLGGDYRLSGIPRMHERPIGDLVDALRTLGCRIDYLGQAGYPPLRIGAPDLSL
                     DRPIPVRGDVSSQFLTALLLALPLAAGRPGARDIVIDVVGELISKPYIDITLQLLARF
                     GVTVQRQGWQRFVIPSGSRYRSPGEIHVEADASSASYFIAAGALSEGAAGQNGLTIEG
                     VGEASIQGDIRFVEAARQMGAQIESGPNWLRVRRGAWPLRAIDLDGNHIPDAAMTLAV
                     MALYADGPSTLRNIASWRVKETDRIAAMANELRKLGAQVEEGADFIRIHPPAGAAAWR
                     AASIHTYDDHRMAMCGSLAAFNPAGLPVRIEDPRCVGKTFPDYFETLFALAGAAGPVP
                     VICIDGPTASGKGTLAAELARRLGFRFLDSGALYRLVGLAARQQGMAPDATNEAALAS
                     LARQLPVRFEAQTVWLADQDVTDAIRTEAAGMDASRVSALPAVRQALLALQRDFARLP
                     GLVADGRDMGTVVFPDARLKVFLTADAAQRAERRYKQLISKGISANIDSLRADLEARD
                     ARDRDRAAAPLKAAPDALLLDNSLLSIAQSVEQVLSWWQARQPF"
     gene            complement(26666..27538)
                     /locus_tag="PKN51_03405"
     CDS             complement(26666..27538)
                     /locus_tag="PKN51_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNT84177.1"
                     /translation="MFEQLGLIGCGLMGGSFALALKRAGLVKRVVGYSKSPSTTARAL
                     QLGVIDVEAPSALLAVSGADLVLLAVPVAATETTLKAIRHLVNGDMLIMDVGSSKRDV
                     VDAARRVLKQDVGVFVPCHPIAGREVSGVEHADAGLYAGKQVILTPIERTLMAKLEQA
                     NALWQALGCQVVNMSPESHDAAFAAVSHLPHLVAFALMNSIVNQPEGREYLSLAGPGF
                     RDFSRIAASDPQMWRDVLIANREELGRQTELFQHALNDLQRLIANGQADDLERLINRA
                     SHARANWRLSSGAR"
     gene            complement(27531..28640)
                     /gene="pheA"
                     /locus_tag="PKN51_03410"
     CDS             complement(27531..28640)
                     /gene="pheA"
                     /locus_tag="PKN51_03410"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNT84178.1"
                     /translation="MSSTTPVQSEALAQLRTQIDSLDAQLLTLLNQRAKVAEQVGELK
                     RHEGTPFFRPDRVAQVIDKIQASNTGPLQNSHVAAIWREIMSACLALEVPQRVAVLGP
                     QGTFCEQAAIEYFGSAANLIYCSSFDEVFHATAAGTAQFGVVGMENTTEGVVARSFDL
                     FLHSPVHVIGEVSLLVRHNLLRQVNALDKIEVVLAHPQALMQCQGWLSEHLPGAERRA
                     VSSNAEGARLAATNPAWAALASERAAAQFGLHIVAHAIQDEAYNRTRFAIICLPQTLG
                     TPAATGRDCTSIVVSVPNKPGSVHDMLTPLKKHGVSMTRFESRPAKSGKWEYFFYIDL
                     QGHPGEPNVAAALSELQAYCAFYKMLGAYPLGQDV"
     gene            complement(28721..29236)
                     /locus_tag="PKN51_03415"
     CDS             complement(28721..29236)
                     /locus_tag="PKN51_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNT84179.1"
                     /translation="MAQNDAKRALANKLVQLQLKTDGPAITDQLTNSAVQPIVAGWSQ
                     RLDETVPPARQKEVRDKLDVELKKFADSTHKSIEAQVGKAAEAAMVPIFMEKLSEDEM
                     KTIIAYMESPASAKLQALGADATDAWAKRIIDSTRSQVEASAKTFESAADRIVKAAAG
                     GASGAAAATKK"
     gene            complement(29314..31971)
                     /gene="gyrA"
                     /locus_tag="PKN51_03420"
     CDS             complement(29314..31971)
                     /gene="gyrA"
                     /locus_tag="PKN51_03420"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNT84180.1"
                     /translation="MTSFAKETLPISLEEEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLYAMHELNNDWNRPYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQDFSLRHML
                     VDGQGNFGSVDGDNAAAMRYTEIRLSKIAHEMLADIDKETVDFGPNYDGSEQEPLVLP
                     SRLPNLLVNGSGGIAVGMATNIPPHNLNEVVDACLHLLRQPAASIEELMEIMPAPDFP
                     TGGIIYGMQGVREGYRTGRGRVVMRAKVHFEDIDRGQRQAIIVDELPYQVNKKTLQER
                     MAELVHEKKLEGISHIQDESDKSGMRLVIELKRGEVPEVVLNNLYKQTQLQDTFGMNM
                     VALIDGQPRLCNLKELVEVFLQHRREVVTRRTVFNLRKARERGHVLEGLAVALANIDE
                     FIRIIRESPTPPVAKAELMARSWDSQLVREMLTRTRADGGVVNADDYRPEGLERACGM
                     QADGLYRLSDTQAQEILQMRLQRLTGLEQDKIIAEYKEVMAQIDDLLDILARPERVST
                     IIGEELSALKAEFGQTKVGARRSTVEHNAQDLATEDLITPTDMVVTLSHTGYIKAQPV
                     SEYRAQKRGGRGKQATATKEDDWIDQLFIANTHDWILCFSNRGRLYWLKVWEVPSGSR
                     GSRGRPIVNMFPLAEGEKINVVLALTGAMRNFPDDHFVFMATSMGTVKKTALPEFSNP
                     RKAGIIAVDLDEGDYLIGAALTDGAHDVMLFSDGGKAVRFDENDVRPMGRNARGVRGM
                     SLEEGQRVIAMLVADGETTTDSAEPNARNSVLTATENGYGKRTNINEYTRHGRGTKGM
                     IAIQQSERNGKVVAATLVSSDDEIMLITDKGVLVRTRVAEIREMGRATQGVTLIALDD
                     GAQLSGLQRIVENDAQDPAIDPTQESPTE"
     gene            32225..32890
                     /locus_tag="PKN51_03425"
     CDS             32225..32890
                     /locus_tag="PKN51_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT84181.1"
                     /translation="MKKLNKVAALFAVATVAGSAFAAGSTINSDNWRNGHGNLIWKNG
                     TNELCWRDAGWTPATAAPGCDGALEAPKAAAPAAAPAPAAAAPAPAPAKAPAPAPALA
                     AQKVTYAADAFFDFDKSVLKPEGRAKLDDLVSKIQGINLEVIIAVGHTDSIGSDAYNQ
                     RLSVRRAEAVKAYLVSKGIERNRVYTEGKGEKQPVADNRTAQGRAKNRRVEVEVVGTR
                     AAK"
BASE COUNT         5102 a        10981 c        11586 g         5291 t
ORIGIN      
        1 catcaggtgc tcgacccaca ccggcccgtc ggccgcctgg aacaccatgc tgaagatggt
       61 ggcgccgatc aggatgaaca tcacgaacga cgacagcttg gtggtcgtgc ccagtgcctg
      121 cttgagcagg tccatgttga tgcgccggcg catggcagcc atgatcagtg cgcccatggc
      181 gcccatggcg ccgccttcgg tgggggtggc cacgcccagg aagatggtgc ccagcaccag
      241 gaagatgagc agcagcggcg gaatcagcac aaaggtcacg cgctcggcca gttgcgacag
      301 cagccccatg cgcgtgatgc ggttgacgag cgcgatcagc caggcgacga aggtgccgcc
      361 gcacatggcg acgacgatct tttcgtcggt gggcacgaac tcgacgggcg tgccctgcca
      421 ccaggtgtgc acggcctcca tgttcctggc cagcaccacg gccacggccg tggacagcag
      481 ggccagcacg cccagcgagc ggtagcccga atggccattg ggctcgcgga aggtgcgggc
      541 ctcgggcggc agggccggca ccatgctggg cttgaagatg gccagcgcaa acacccacag
      601 gatgtacagg cccatgagcg caaagccggg gatgaaggcg cccttgtaca tgtcgcccac
      661 gctcttgccc atctggtcgg ccagcacgat cagcaccagc gatggcggga tgatctgcgc
      721 cagcgtgccc gaggcggcga tcacgcccga ggccaggcgt cggtcatagc catagcgcag
      781 catgatgggc atggagatca ggcccatgga gatcaccgat gcggccacca cgccggtggt
      841 ggcggccagc atggcgccca ccagcaccac ggccagcgcc aggccgccgc gcagcgggcc
      901 gaacacctgg cccacggtct cgagcagatc ctcggccatg ccgctgcgct ccagaatcag
      961 ccccatgagg gtgaagaagg gcacggccag cagggtctcg ttgctcatga tgcctatgag
     1021 gcgctgcggc agccaggaat agatggcgct ggtgaacacc cccagctcca cgcccaccag
     1081 ggcaaagaac agaccggccg cgcccaggct gaaggccacg ggaaagccca gaatcaggaa
     1141 cacgatcagc ccggcgaaca tcaccggggc aaagttggca gcgatgaatt ccatggtgca
     1201 gatgctccgc gtgcgcgtca gtgggtctgg ctggcgctct gctgctggcg cagcgcgtcc
     1261 gcgagctcct gctcggcggt cttgtccagg gccttcacgg tcggatccgg cccctggccg
     1321 cgcaggaagg cgatgcactt gatcagctcc gaccagccct gcagcagcag cagcacgaag
     1381 ccgatcggca gcgccgccca caccggccag cggatcaggc cgccggggtt gggtgacatc
     1441 tcgccggagg cgatccgttc gaacacgatc ggcgtgctca tccacagcac gatgacgcag
     1501 accggcgtca ggaacagcac atagcccagg atctcgatcc acagctgcac gttcttgggc
     1561 aggcggctgt tgagcacgtc gatgcgcaca tgctcgcggt gcagcagcgt gtagcccgcg
     1621 gcggccagaa acgaccaggc gaacagatac cactgcacct cgaggaaggc gttggagctg
     1681 gtgtcgaagg ccttgcggac caccgcgttc agcgcgctga tgatggtggc accgaagatc
     1741 agccagatga cgaaacgacc gagcgaggca ttgagccagt cgacggcacg cgaaaacttg
     1801 agcagcgctt gcatcgggat ctccgtgagg gggcgtggtg cgcagggtgg gcgcgaccag
     1861 gggcggcggt aaccgggtgg gccatgccgc agacagatcg caggcgattg taatgaggcc
     1921 cctggggcgg cgccgtatag catcggcacg gtctgcgcca ttcgacacca tgagcaccct
     1981 ggccgaaagc gcgcgcgccg cgctgcaact catcgcttcg ctggacccgc tgctgtggtc
     2041 ggtggtgggc cgatcgctgg cggtcagcgg gctggcctgc ctgctggcct gtggggtcgg
     2101 gctgctggcc ggtgcatcgc tcgcggtggc gcgctttcgc gggcgcgagg ccgtgctgac
     2161 gctgctgcac accctgctgg ccctgccgtc ggtggtggtc gggctgctgg tgtatctgct
     2221 gctgtcgcgc agcggcccgc tgggttttct gggttggctg ttcagctttc aggccatggt
     2281 gctggcgcag gcggtgctgg tggtgccggt gatcgccgcg ctcacgcgcc agacggtgga
     2341 agacgccgac ctccggcatg gcgagcagct ggcgtcgctg ggtgccggcc cttgcctgcg
     2401 cgggttgctg ctggcctggg acgagcgctg cgccctgctg atggtgctga tggcggcgtt
     2461 tggccgggcg gtgtcggagg tgggcgcagt gatgatcgtc ggcggcaaca tcgacggctt
     2521 cacgcgggtg atgaccaccg ccatcgcgct ggagaccagc aagggcgatc tgccgctggc
     2581 gctgggcctg ggcatggtgc tgctgtcggt ggtgctgctg ctcaacgccg ccatggccgg
     2641 cctgcgccat tggcgagcgc gcaccgacgc tctggccccc ggcgcggtgc agggacggtg
     2701 ggccgcatga gccgtcccgc cggccccccg ggcgcagcgg cgccgctgat ccgcctgcag
     2761 gatgtgggcg tgcgcttcgg ccgggtgcag gcgctgcagg gcgtggggct ggagatcgcc
     2821 gccggcgagt gcgtggccct gatcggcggc aacggcagcg gcaagagcac gctgctgcgg
     2881 gtgatccacg gtctgatcgc gccgaccgag ggccagctgg tggtgccggc gcgccgggcg
     2941 caggcgatgc tgtttcagcg cccctggatg atgcgcacca cggtgctgcg caacgtcgcg
     3001 ctcgggctgt ggctgcgcgg cctgccgtgg cgccaggcgc aggagcgcgc gttgcgggcg
     3061 ctggagcgcg tgcaactggc cgaactggcg tcgcgccgcg cgcccggcct gtcgggcggg
     3121 cagcagcagc gcgtggcgct ggcgcgtgcc tgggcgcagc aggcgcaact gctgttgctg
     3181 gacgagccca cggccagcct ggacccgcac gccaggctcg aggtcgaggc gctgatgcgc
     3241 gcgttcaccg gcacggcgcc cgacgcggcg ccgttgacca tggtgttcgc cagccaccac
     3301 ctgggccagg tcaggcgcct ggcgcagcgg gtcatctgtc tggaccaggg gcgcgtgctg
     3361 gccgatctgc ctgtggcaca gttcttcgac cacgcgcggc tgagctcggt ggcgccggtg
     3421 gcggcggcct tcgtgcaagg agaaatgtga tgcaaaaatt cagaaacttt tggcctctgg
     3481 cgcttttgca gactgcgctg gcagctatct taacgatagc atttccggtg gcctgggcgc
     3541 aaaccatcgt gatggcctcg accacctcga ccgaacagtc ggggttgttt gcccacctgc
     3601 tgccgcagtt ccgccaggcc agcggcatcg acgtcaaggt ggtggcggtg ggcaccgggc
     3661 aggcgctgga catcgcccgg cgcggcgacg ccgatgtgct gttcgtgcat gacccgaagg
     3721 ccgaggagca gttcgtcacc gacggctggg gcctcaagcg ccacccggtc atgtacaacg
     3781 acttcgtcct ggtcggcccg cgcagcgacc cggccggggc gcgcggccgc gacatcgtgc
     3841 aggccatgcg gcagttggcg ggggcgcacg cgcctttcat ttcgcgcggc gacaaaagcg
     3901 gcacccacag cgccgaactg cgctactggg cccaggcggg cctgaccgaa gcgcgcgggc
     3961 gtggctatcg cgaatgcggt tgcggcatgg ggccggcgct gaacatggct gccgccagcg
     4021 gcgcctacgt gctggccgac cggggcacct ggctcaactt caggaaccgg gccgacctgg
     4081 caatcctggt cgaaggcgac ccgcgcctgt tcaatcccta cggcgtgctg gtggtcaacc
     4141 cggcacgcca cccgcaggtc aaggccgccg aggcacagaa gtttgtcgac tgggtgacgt
     4201 cgcccgccgg tcaggccgcc atcgccagct accgcatcgg gggccaggcg ttgtttttcc
     4261 cggacgcgcg accttgaggc ggcgtctgac cccctgaaca ggtgactgga ctcgcagaaa
     4321 atgaaataat aagtatccag ttacactttc aggagggttg gtcatggcat gtttcccttc
     4381 ctggcggacg ctctgccacc atgcgttggg cgttgcgctt ggcctgctgc tgctgccggc
     4441 gcaggcccag atccgggtgt cgatcaatcc gggcgaccag atcgaggccg atcgcgtggg
     4501 catcttcaac gcctggaagg gcgcgctgga tgccgggctg cgcaaggccg gcgccagcgc
     4561 ggcgcaggcc cgcttttcca gcgacgccac ggccgacatg aatgccggcc gcgccaacgt
     4621 gttcgacctg atggtggggc cggcgccggt catcggcagc gccgtgcgct acggctacgt
     4681 gccggtggtc ggcgccaccc agaaggcgcg cgccgtgctg gtggcggccg agggcagcgc
     4741 catcgccgac tgggcccaga gcaagggcaa gcgcctgggc atgccgggcc aggacagcgt
     4801 ggtgacctat ctgctgcgtg gcgaggtgca ggccggcaac accacgctgg cacgccagta
     4861 cccgtcgatc taccagacac gctaccagga cgccctgctg acctgcctgg agctgcgccg
     4921 ctgcgacgtg gtggcggtgg aggaggtcgt ggccaagcgc tgggcggcga gcggcaagaa
     4981 ggtcaaggtg gtctggcaga gccgcgaggt gccgggtctg agcgtggcgc tgcacaagga
     5041 cgcccaggtg tcggccgaca ccctgcgcag cgcgctcatg gccgagctgg ccaggctcga
     5101 tgcctttggc tacaccggca cgttgacggt caaggatttc gagtacgtgt ccacgctggg
     5161 ctacttcacg ccgcgcaccc tggaaggcgc gaccctgatc gaagacccgg cgcaggtgga
     5221 gtcgctgctg gccgctggcg cgcgctatat cgacgcacgc aaccaggaag agttcgccgc
     5281 cggccatgtg ccgggcgcca tcttggtgcc gtacgtcgaa aagagcgcca aggatccggg
     5341 ctacgacccc aagctggatc agttcgacct ccagcgcctg ggcgacaaca aggcgcagcc
     5401 gctggtgttc ggctgcaacg gcccggaatg ctggaagagc ttcaaggcca gccgcgccgc
     5461 cctgcaggcc ggctacacca aggtgtactg gttccgcacc ggtgtgccgg cctggcgcag
     5521 ttcgggccgc ccgctcgatc gtggtgacgc caaggcgggc tgaacggggc gtcagtcgag
     5581 caccgccacg cccttgatct gggcatacac cgactgcccg ggcgccagcg ccagcgcctc
     5641 ggccgatttg cgcgtgacgc gcgccagcag cggtgtggcg ccgaggtcca gcgccaccag
     5701 cacctgcgcc gggccgtctt cggccagcga acgcacgcgg gccggcagca cgttgaggat
     5761 gctggtgtcg tgcgcggcgg acaggcacag gctgacgtca cgcgcctgca cccgcaggcg
     5821 cacgcgctgg cccggcgtgc gcggcggggt gccggcgctg tgcacgcagt gcaggacacc
     5881 gtgatcgaac gccacctgca gcaactggta gtgcgggtcg aagctcagca cctgtgcgct
     5941 caccagcgcg ctggcggcat cgccctgggt gcgcgccacg tccgggcgcg tcagcagcgc
     6001 ctcgaccggg ccgtgcgcga cgacctcgcc atcgcgcagc agcaccaggt ggtcggccag
     6061 gcggatcact tcgtcgagcg catgggtgac gtacagcatc ggcaggcgca gctcgcgcgt
     6121 cagccgctcg aagtagggca gcagttcggc cttgcgtgcg gcatccagcg aggccagcgg
     6181 ctcgtccatc agcagcaggc gcgggctggt ggccagcgcg cgcgcgatcg ccacccgctg
     6241 gcgctcgccg cccgacagct gcgccgggcg gcgtgccagc atggggccca ggcccagcag
     6301 ctcgatcgcc tgcggcagcg ccacgcgccg ctcggcggcg ggcacgcgct gcagaccgaa
     6361 gtccaggttg ccctgcaccg tcaggtgctc gaacaggctg gcttcctgaa acaccaggcc
     6421 cagcgggcgc cggtgggtgg ggcgccagat gcggtgcgcg tcgtcctgcc acacgtcgcc
     6481 gcccaccgcc acgcgccccg gtgacggccg cgtcagcccg gccacggcgc gcagcagcgt
     6541 ggtcttgcca cagcccgagg ggccgaacag cgccgtcacg ccctggcctg gcaggcgcag
     6601 ctcaaccccg agccgaaagc cgctgcccat gcgcacctgg gcgcgcagtt cgatgtcgtg
     6661 gccggtgccc tgcgtcatgc cggcgccttg cgcgattgcg tcaggtgcag ggtcagcagc
     6721 accaggaagc tgaacaccag catgccgccg gccagccagt gcgcctgggt gtattccagc
     6781 gcctcgacat ggtcgtagat ctgcaccgag atgacgcgcg tctggccggg gatgttgccg
     6841 ccgatcatca gcaccacgcc aaattcgccc agggtgtggg cgaagcccat cacggcgccg
     6901 gtcaggtagc ccggccgcgc cagcggcagc gccacatgca ggaacgcatc cagcgggctg
     6961 gcgcgcagcg tggccgcggc ctccagtggg cggcggccga tggcttcgaa ggcgttgacc
     7021 agcggctgca ccacgaaggg cagcgaatac accagcgaac ccagcaacag gccccagaag
     7081 ctgaacgcca gcgtgcccca gcccagcgcc tgcgtcaggc ggcccagcgg accctgtggc
     7141 cccagcatga ccagcagata aaaccccagc accgatggtg gcagcaccag tggcatggcc
     7201 accagcgtgc gcaccggcgt gcgcaaccag gccagcgcgc ccgcgtcgcc gcgcgccagc
     7261 caccacgcca ggggcgtgcc cagcaccagc aggatgcagg tggtggccag cgccagctcg
     7321 atcgagagcc ggacggcggc aaggctggtg gcgtcaagca tggggcaaag caaatgctca
     7381 ggcggcgccg gggcgccgcc gtaactcatg ttttgatagc tgttggcgct tgatgtgcaa
     7441 gcgccgaagg cccattttgc cttgaagggc ttacttcacg gcgtagccgt agctgcggat
     7501 caggtccttg gtgcgcggcg agctcagcag cgccatccag gctttggccg cggggttgcc
     7561 ctgtccgcgc ttgaggatca cggcgtcctg cacgatgggc gcgtgcagcg agcccggcac
     7621 cacccacatc gaaccaccct tgagccgccc accctgcagc acctgcgaca tggccacgaa
     7681 gcccaggtcg gcgttgccgg tggcgacgaa gttgtaggtc tgaccgatgc tctcgccggt
     7741 caccagcttg ggctggaggg tcgcttgcag acccagccgg ttcatggcct cgacggcggc
     7801 cgcgccgtag ggcgcattct tggggttggc gatcgccagc ttgttgaact ggccgccttg
     7861 gagcacctcg cccttgtcgt ccaccaggcc gggtttggcc gaccacaaca ccaactgacc
     7921 ggtggcgtag gtgaagcgcg agccggggac ggtgtcgcct tccttttcca gccgcgcggg
     7981 gcgctcgtcg tcggccgaca ggaacacgtc gaacggcgcg ccgttcttga tctgcgtgta
     8041 gaacgccccg ctggcgccga acgacagcct ggcctcgtgt ccggtggtct tttccagaat
     8101 ggcggccagc gccttggccg gttcggtgaa gttggccgcc acggcgacgt ggaccgtttc
     8161 ggcgtgcgcg gaccacgagg ccaccatcac ggcggccagg gtcacaaggg tacggatggg
     8221 acgcatgggg gaactctcct ggaagccggc gcggcggcgc cggtagcgtt attcaacttc
     8281 gggacaacga gtctaactca gggaacgaag ggatcggcga actcggggcg cgcaagcatg
     8341 cggtcgtccg tcagcgcacg caggaaggcc accaggtcgg cttggtcctg cgcgtcgatg
     8401 tcgatctgat cgatccgcgg atccttgtac gggttggcgc ggccgtcgcc ttcgtacggg
     8461 ccgttgccga gcacgcggcc gccggcggca tgcagcgcca ccaccgcttc cagcgtggcg
     8521 atgctgccgt cgtgcaggta gggtgcggtc agcgcgacgt tgcgcaggct gggcgcgcgg
     8581 aacctgccca tgtcctcggg cctgccggtg aattcgagca aaccccggtt gttggtcggg
     8641 taagcaccgg tgccgccgat gttgaacagg ccgatgttgt agaaccccgg ctcgcccacg
     8701 cggccctggg cgctgacgaa cgcaccgccg cccaggttgg ggccgctgtg gcactgcgcg
     8761 cagagcgcgc gctcgccgaa gaacaggcgc tggccgcgct gctcgctttc agtcagcgtg
     8821 gccgtgccgg ccagcgcctg gtcgtagcgg ctgccggccg ccaccagcgt gcgctggaag
     8881 gcggcgatgg ccttgatcac gttgtcccag tggatgggca tcgcttcgcc gccaaaggct
     8941 tgggcgaaac gcggcgggta gcgtgcgtcg tccttcaggc gctgcacgat ggcgtcgcgg
     9001 ttggtgtcgt tcacgcccat ctcgaccggc tgctcgccaa acaggggcgt gtgcatctgc
     9061 tgctccagcg tgcgcggcgt cgggttgctc cagtccagcg tggcctggta ggcggcgttg
     9121 accaggctgg gtgcgttgcg cgggtggttc tgcccggtgg cgccgcgcgc cacggcgcgg
     9181 ccgtcggcga aggccaggtt gacggcgtgg cagccggcgc aggacaacgt gttgttgacc
     9241 gacaggcgca cgtcgtagaa caggtggcgc cccagctcca ccttggctgc attcatcggg
     9301 ttgtcggccg gcaccagggg cggcgccagg cctgccggca gtgaccaggc ccaggctggc
     9361 ggcaccggcg gcggtcctgc atcggcgctg tccccgccgc cgcaggcggc aagcgacagc
     9421 agggcaagtg cggcagcgac gcgagcgcgc agcggtgtgt gcgtcatggt gggttcctcg
     9481 tgggtatcga acgtcgttat tcaccgatag aatagcgcat cactccacct tcaccacgat
     9541 agaaaaggac gaaccatgaa catgacggca cggcaggaca cggactcttt ctgggcgcgc
     9601 gcgcggcgcg ggctgctggt ggccaccggg tttgccttgc tgtcgcttgc cgcctgcggc
     9661 ggcaacggcg acgacgaggt ggccgaccgt ggcccgacca ccctcgaggt ccaggccaag
     9721 ctcaacggcc tgtactggga tgcgcaggct tcgcgtctgt acctgaccga cgacgaggca
     9781 agcgtcaatg ccatcagaat ctgggacggc gacgacaagt tctccgtggc gcaccagctg
     9841 ccggctatgg aggccaatca gcgtcactcg ctgggccagc tcacgcgcgc tgcggacggc
     9901 aagctgtacg tcacgcgctt cggcttcggc agctacggca cggtggtcgc cgtgcccaag
     9961 tcgggccaac cctacaacct gacgggactg gccggcgacc gccggcgcat cgccatcacg
    10021 cccacggccg acggcaaact gatcgacggc tggttccggg gtggcggcgc gcccggaccg
    10081 agcggcgtga tcagcgagct gaccgtggcc gccggcgggc aggccagcga gcaggacctg
    10141 atcacgggcc tcagcaagcc tgccggggtg gcggtggtgg gcgaccagct gttcgtgtcc
    10201 gaccagggca cgggcaaggt gctggcctat tcgctggccg ccgtgcgggc gcagccggcg
    10261 aaggccgagg atgggcgcgt cgttgccgag ttcatctccg ccgacagcat cgatctgatg
    10321 acggccgggc ccgatggcac gctgttcttc ggcggcggcg ccggcacgct gtacgcggtg
    10381 gcgccggacg gcaaggtcag gacgctggcc agcggctggc ccggcatccg cggcgtggcc
    10441 tacgacgcgc agaacaagcg cctgttcgcg gccgtgacgg cgcccaacgc cgccagcccg
    10501 gccaccatcc gcatcgtgcc gatcgattga acctcgacac ggcagttgac cgcttgtcat
    10561 catcgggcag gccgcatccc tgcagggttt cgcggctgcg atggccttca cctcttgggt
    10621 gagcacgctg cgcacccgcc cgcaccgcgc tcgccgcgcc atgcggcgtt gctcccttgc
    10681 gggcacgagc ccgccgcggc ggcgtgtctt gcctggcacg ccgatcacgg cgcgggcggg
    10741 cgcatccaac tgccatttcc aggttgaagc gcctcgcggc cgccgtgcaa gcggcagtgt
    10801 tcgcctatgt ctgcgagaca atgggcgaca tgacgaaaaa gaccttgtcc gcctccagtc
    10861 tgggttatgg ccaggccgac aagcgcatcc tgatcctgca actggtgggt gcgcacggct
    10921 ccatctcgca ggcggcgcgg gtggcgggcg tcagctacaa ggcggcctgg caggcgctgc
    10981 acacgctgac caacctggcg ggtgaggcgc tggtggacag cagcgtcggc ggtgccggcg
    11041 gcggcggcgc gcgcctgacg cctgccggcg aacggctgtt gcacgcggcc acccgcctgg
    11101 cgcaggcgcg ccaagccgtg ctgtcgcaac tgggcgccgc cgacgacgcc atcgccgcgc
    11161 cacgcaccag catgcgcaac catctgcgcg cccgcgtgct cgaactggtg ggcgccggcc
    11221 cgcgcgaacc catggtgcgg gtgcgcctgg cgctcgatgg tggcgccgag ctggcggcgc
    11281 tcatcacgcg cgagagcgcc gagctgctgg gcctggcacc cgggctggcg ctgctggcgc
    11341 tgaccaaggc caccgccgtg cgggtggtgc ccgccgccag cggggcgccg cggccggggg
    11401 tgctgccggg gcgcgtgctg cgggtgttcc gcggcaccca cggcgacgag gtcacgctga
    11461 ccctgacggg tggccagcaa ctggtgggat tcgccgagcg gcccaaccgg ctgcgtgccg
    11521 gctcagccgc cctggcgcag gtcgacgacc gcgtggtggt gctggcgctg ccgggctgac
    11581 cgcgcgaaac actgcactag cgcccgtcca tcaagcgcga gcagctatca aaaccagagc
    11641 ggtccggatg ccggcgaagg tcagcagcag cgagcccgac aggtgcgcca gcacggtgac
    11701 cgcggccagc ccccagcgtt gcgccagcgc catgtccacc acttcggcgc tgaagctgga
    11761 aaaggtggtc agcgcgccca gaaagccggt caccagcagc agccgccagg tcgggtcgag
    11821 ctgcgggtga tggtgcagca gcgccaccgc cagcccgacc aggtagccgc cgatcaggtt
    11881 ggccgccagc gtgccccagg gcagccaggc gccagcgtgg ttgagccaca ggccgagctg
    11941 ccagcgcagc agggcgccgg cgcaggcgcc cagacagatg gcgagaacgg cgtgcatcgg
    12001 ggttgcgggc gcttggccgg ctcagcgcgt gctcagaacg gcgggtcgtc ggcggcgacc
    12061 gtggcttctt cggtcacggt gctggccggt gtgggcgctt cggtgaagcc cgagggcgtg
    12121 ccgggttcga tctcgcctcc cagggcggcg atcaggtcgg tgatcaggcc gcgcagctcg
    12181 ccggtggcca gggccacgtc ggcgtcaaag cgttcgtcgg cgctggccgg cgccgggcgc
    12241 tcgtcgaaca cgccgtccag aaagccgatc ttcttcagtt gcagcgcttg cgtcagcacg
    12301 aagccgacgc ggccctgcca gccaagcgcc agccgcgtgg gcagcttgcc ttcggcgatg
    12361 tgctggcgca cctcgtcggt ggccagctcg tggcgggtga acttgaccac cgccggctcg
    12421 tcgccactgc ccttgagctc gcactcgcgc tccacgtgca ggccctcggg ccagtcgtcg
    12481 gccgaggtgg cggccagcca gccggtcatg gcggcctgcg gcgagtgcgc cgtctgcagc
    12541 aggtggatgg caaagccctg gccggccaca cgcaccaggc tggcaatcac ttcgtcggcc
    12601 ttgccctggc tgccggcgtc cagcgccagc cagcgctgct gcgggttcag ccacagcgtc
    12661 acctggctgc ggcgcgcaaa ggcctgcggc agcagggcca gcagggcgtc gtcgcgcagg
    12721 tcgcgcagct ccttcttgcc gggtttgcgg ccgctggtct tttcgatctc ggccaccgcc
    12781 tgctgcacct tgcggcgcac ggcgtcgccc ggcacgggct tggtttcgat ggcgaagcgg
    12841 gcgatccact ggccgtccac cgcctcgacc agcgcgccat gctgctcgcc gcgcggcgcc
    12901 acccagccgg tggatttctg ctgcgtggcg ccgcactcgg caaacggctc gcgcgccagc
    12961 gctttttcga gctgcgccgc cgatgcgggc caaccggggc ccaggcgata cagcatcaga
    13021 ttcttgaaca cggatagtca acctgtctca aacacccccg aaggggccgg accggcatct
    13081 tagccacatc gcttgcgccc accccgcatg ggtccgatgg ccgcggagca ggccaggcgc
    13141 caacgccccg gaggaggcag ggccggcatc gtcgccacgc aactcagtgg cagatggctt
    13201 gctccgcaca ggtcgaacgg ccgcggagca ggtcaagcca gggctcccgt ggccggggtc
    13261 ccctcggcca gtgggtgcgt cccccccctc cagggggaag gcgccgcagg cgacacaggg
    13321 ggggttacat gttcttcggc agccatgtgg cgatctgcgg gaagaaccac agcagcagca
    13381 ccatggcgca catcaggaag aagaagggca gcgtgacgcg cgcgatgtag gcgatatcgt
    13441 gcctggtcat gccctgcagc acgaacaggt tgaagcccac gggcggcgtg atctgtgcca
    13501 tctcgaccac gatgacgatg aagatgccga accagatcag gtccagcccg gcggcctgca
    13561 ccgtcggcag gatcacgccc atggtcagca ccaccatgga gatgccgtcc agaaagcagc
    13621 ccagcacgat atagaacacc gccagcgcca gaaccagcat gaagggcgac aggcccaggc
    13681 cgccgatgaa ttcggccaga tggcgcggca ggccgatgta gcccatggcc agcgtcagaa
    13741 aggcggcgcc ggccaggatc agcgcaatca tgcagtacag ccgggtggcg cccagcagcg
    13801 cggtcttgaa ggtgctccac gacaggctgc cctgcaccag cgacagcagc agcgaaccga
    13861 tcacgcccac ggccgccgcc tcggtggcgg tggccacgcc ggaatagatg gaacccagca
    13921 cggcggcgat cagcagcacc accggaatca ggtggcgcga ttcgtgcagc ttctcggcca
    13981 ggctcaggcg ccggtcgggc atggggatct gatcggggtg cagcagcgcc cagaccatca
    14041 gatagcccga gaacagcgct gccagcagga tgcccggcag gatgcccgcg atgaacagct
    14101 tggcgatcga cacgtcggcc gtcacgccgt agacgatcat gatgatcgag ggcggaatga
    14161 gcaggcccag ggtgccggcg ccggccagcg agccgatggt gatgtcgcgc gggtagcccc
    14221 ggctgcccag ctcgggcagg gtcatcttgc cgatggtggc gcaggtggcg gccgacgagc
    14281 ccgacacggc ggcaaagatg gcgcagccga tcacattggt gtgcagcagc cgcccgggca
    14341 gccaggtcac ccacggcgcc aggccgcgaa acatgctttc cgacagcttg gtgcgaaaca
    14401 ggatttcgcc catccagatg aacaggggca gggcggtgag cgtccagctg gaggccgagc
    14461 cccagatggt gaccgccatg gcgtcgcccg ccgggcgggc cgagaacagc tgcatgccga
    14521 tccaggccac gccggtcagc gtcaggccga tccacacgcc gctgcccagc agcgcgaaca
    14581 acgacaggat cagcaggccg gtgatggcga agtcactcat tgtgcagcgc ctcctcgtcg
    14641 cccgactcgt gctgtcgcag gccgcgcagc tccagcaccc attcatcgat gaaggccagc
    14701 agcaggatca ccgtgcccac ggccatggcg atctgcggta tccacagcgg cgtggcgtcg
    14761 ttgctggtgg acatgtcgtt gaacaggtgc gactgccaga ccagccgcac cgaaaaccag
    14821 gcgaacagcc ccgccagcag caccgccacc gacagcgccc acagctccat gccgcgccgc
    14881 gcgccgccgc gcaggcgccc catcagcagg gtcacgcgga tgtgctcgtt cttcttgagc
    14941 gtgtgcgcca gcgacagaaa gcccgccgca gccatgctgt agccggcgta ggcgtcggta
    15001 cccggcaggt gaaagtcgag cagacggccg aggatggagg tcagcaccat cagcagcacg
    15061 ccgaccatgg ccagcgccgc cagccaggcc gccgcgtcgt agaggaaatc gagcaatcgc
    15121 cgcacgttgg ctccttggtc gggttgcgtg gtctgggctc aaaaaaactc ccgccgggcg
    15181 aacccggggg gagtgtgcca cggggtgcgc gggcgcttac ttggcggcgc ggtaggcgtc
    15241 gaccacggcc ttgccctcgg cgccggcctt gtccagccat tccttcagca tggtgtcgcc
    15301 caccttgcgc atgtcggtca tcagctgggc cgagggggcg tacaccgtca tgccgttctt
    15361 gcgcagcagg tccaggtatt cgttggtctt gcgctcgctg gtggcccagc cgcgggcctc
    15421 ggcgtcggcg gcggccttga gcaaaccgtc ctgggtggct ttgtcgagcg cgtcgaaggc
    15481 cttcttgttg gcgatcacgg cgttcttggg cagccaggcc tgggtgtcgg caaagaactt
    15541 caggtgctcg taggtcttgg tgtcgtagcc agtggagccg gagctcatgt agctttccac
    15601 cacgccggtg gccatggcct gcgacagctc ggcctgctgc accgtcaccg gctgggcgcc
    15661 caccagctcg gcgatgcgcg cggtggccgg gctgtaggcg cgccacttga tgcccttgag
    15721 gtccgccgcg ctgccgacct gcttcttggt gaagatgccc tgcggcggcc aggccacggc
    15781 gtacagcagc accatgccct gttcggccag cttcttctgc accgtcgcct tgctggcctg
    15841 gtacagcttc ttggattcgg cgtagctggt ggccaggaag gggatgccgt ccaggcccca
    15901 caccggccac tcgttctgga agttgaccat cagcagctca ccggcctgcg cctgcccgcc
    15961 ctgcacggcg cgcttgatct cgggcgcctt gaacagcgag gcgttgtcat gcacctggat
    16021 cttcagcttg ccaccggtgg cggcgtccac gtccttggcg aactgcacca ggttttcggt
    16081 gtggaagttg ctggccgggt aggccgaggg caggtcccat ttggtctggg cagccgctga
    16141 aagggcgaag ccgcccaggc tcaacacggc gagggcgccc agggtgcgtc gggtcagggt
    16201 catgtcaact ccttgatggg aatgaaaaag gcgtgccgcg agcatagacg ctggcggcgc
    16261 ccgcgcaaac cgtggaaaca ccaccccgct gccgcgcgcc gtggcgcggc acggcgcgaa
    16321 taatgcggcc tcatgggcgg gccgctgccg tgctgccgag ggcgcccgga gtgctatgaa
    16381 ctagatagct tattgcgcac gccagttgtg cgttagaacc ctgttttatt gaaagatcat
    16441 gatgtcggaa catgctctcc acgcaacacg ctggcagttc tggatcgacc gcggtggcac
    16501 tttcaccgac atcgtggcgc gccgccccga tggcaccttg gtgacacaca agctgctgtc
    16561 ggacaacccc gagcagtacc ccgatgcggc ggtggccggc attcgccagc tgctggggct
    16621 ggccagcggc gtgcccatca cgccggcgca ggtggcctgc gtcaagatgg gcaccacggt
    16681 ggccaccaac gcgctgctcg agcgcaaggg cgaacccacg ctgctggtga ccacgcgcgg
    16741 ctttcgcgat gcgctgcgca tcgcctacca ggcgcgcccg cgcctgttcg atcgtcacat
    16801 cgtgctgccc gagctgctgt acgccgccgt ggtcgaggcc agcgagcgcg tggatgccga
    16861 tggcgcggtg gtcctgccgc tcgacgaagt gctgctgaaa caggagctgc tcgcgcagta
    16921 ccagagcggt ctgcgcagcg tggcgatcgt gttcatccac ggctggaagc acgtcgcgca
    16981 cgagcaggcg gcggcgcgcc tggcgcgcga gatcggcttc acccaggtct cgaccagcca
    17041 ccagaccagc ccgctgatga agttcgtcag ccgcggcgac accacggtgg tcgatgccta
    17101 cctgtcgccc atcctgcggc gctacgtcga tcgggtggcg gccgagatgc cgggtgtgcc
    17161 cctgtacttc atgcagtcct cgggcggcct gaccgacgct cacgccttcc agggcaagga
    17221 cgccatcctg tccgggccgg ccggcggcat cgtcggcatg gcgcgcaccg cgcagctggc
    17281 ggggcagcag cgcgtgatcg gcttcgacat gggcggcacc tcgaccgacg tgagccattt
    17341 tgccggccag ttcgagcgcg agttcgagac ccaggtggcc ggcgtgcgca tgcgcgcgcc
    17401 gatgatgagc atccacaccg tggccgcggg cggcggctcg atcctgcgct ttgacggcac
    17461 gcggtatcgc gtgggtcccg aaagcgccgg cgccaacccg ggcccggcca gctaccggcg
    17521 cggcgggccg ctggcgctga ccgacgccaa cgtgatggtg ggcaagctgc agcccgcgta
    17581 ctttccgcgc gtgttcggcc ccgcggccga tgcgccgctg gacgccgagg tggtgcgcgc
    17641 gcggtttgcc gagatcgccg cgcaggacgc cggcggcggg cgcacgccgg aggaggtggc
    17701 gcacggcttc atccagatcg ccaaccagca gatggccagc gccatcaaga agatcagcgt
    17761 ggcgcgcggc tacgacgtga cgcgctacac cctgcagtgc tttggcggcg ccggcggtca
    17821 gcacgcctgc gccgtggccg acgccctggg catgaaccgc gtgctggtgc acccgctggc
    17881 gggcgtgctt tcggcctacg gcatgggtct ggccgaccag acgctgatgc gcgagcgtgc
    17941 gctggagctg ccgctggaaa tcgggcagct cgctgcgctg cgcgccgccg gcgacgcgct
    18001 ggcggcggac gcgcgcgcgc agctgcaagg ccagcacgcg ggcggcggcg ccatcttggt
    18061 gcaccagcgc ctgcacctgc gctacgccgg caccgactcg gccctggtgg tgcccttcga
    18121 ccgctcgttg gacggcgtgg gcgccgccgc cgcgctgcag gccatcaccg aggccttcga
    18181 ggcggcctac cgccagcgct ttgcctttct gatgcagggc aaggcgctga tcgtcgaggc
    18241 ggtgtcggtc gaggcggtga ttgccggcga agcccccgcc gaaatcgagc agccgctgca
    18301 gccgccgcgc tccgtgccgc ggcgcgacac cgtgcgcatc tacaccagcg gcagtgacgg
    18361 ccagagcggc tggcaccagg ccgcgctgat cgtgcgcgaa gacctgcgcg ccggcgacgt
    18421 gatcgaaggt cccgccatca ttgccgagcg caacgccacc acggtgctcg agcccggctg
    18481 gcgtgcccgc ctgacggggc tggaccacct gttgctcgag cgcgccgccc cgcgcgcggc
    18541 acgctttgcc gccggcaccc aggtcgaccc ggtgctgctg gaggtgttca acaacctgtt
    18601 catgaacatc gccgagcaga tgggcctgca gctgcagaac accgcctact cggtcaacat
    18661 caaggagcgg ctggacttct cgtgcgcgct gttcgacgcc gcgggccacc tgatcgccaa
    18721 cgcgccgcac atgccggtgc acctgggcag catgggcgag agcatccaga ccgtgattgc
    18781 gcgcaacccc gacatgcagc gcggcgacgt ctatgtgctg aacgacccct accacggcgg
    18841 cacgcacctg cccgacatca cggtgatcac gccggtgtat ctggacggcg aggcgcggcc
    18901 cgtgttctac gtcggctcgc gcggccacca cgccgacatt ggcggcatca cgcccggctc
    18961 gatgccgccc ttttccaccc gcatcgagga ggagggcgtg cagatcgaca acgtcaagct
    19021 ggtcgatcgc gggcggctgt gcgagcgcga ggtgctggag ctgctggcca gcggtgaata
    19081 cccgagcagg aacccgcagc agaacctcgc tgatttgaag gcgcagatcg ccgccaacga
    19141 aaaaggcgtg caggagctgc gcaagatggt cgatcagttc ggcctggatg tcgtgcaggc
    19201 ctacatgcag cacgtgcagg acaacgccga ggaatccgtg cgccgggcca tcacgcgcct
    19261 ggccgcgcgg gtgaaggatg gtcatttcac cctgccgctg gacaacggcg cgcagatcca
    19321 ggtggcggtc gccatcgact ccgccacgcg cagtgcgaag atcgactttt ccggcacctc
    19381 gccccagcag agcaacaact tcaacgcgcc caccgcggtg tgcatggcgg cggtgctgta
    19441 cgtgttccgc atcctggtgg acgacgacat tccactcaac gccggttgcc tcaagcccat
    19501 cgaggtgacc attcccgcag gcagcatgct gcgccccgag ccgccggcca gcgtggtggc
    19561 cggcaacgtc gagacctcca cctgcatcac caacgcgctg atcggcgcgc tgggcctgat
    19621 gggcgccagc cagtgcaccc tcaacaactt cactttcggc aacgagcgct accagtacta
    19681 cgaaaccgtc tccggcggct cgggtgcggg cggcgaattc gacgaccagg gccggctggt
    19741 gggcggcttt gacggcacca gcgtggtgca gacgcacatg agcaattcgc gcctcaccga
    19801 tcccgaggtg ctggagtggc gctttccggt gcgcctggag agctacgaga tccgccacgg
    19861 ctcgggcggc gccgggcgct ggcgcggcgg cgacggcggc gtgcgccggc tgcgctttct
    19921 cgagcccatg accgccagca tcctcagcaa cggccgcgtg cacggcgcct tcggcatggc
    19981 gggcggcggg gcgggcgcgg tgggcatcaa tcgggtgctg cgcgccgacg gcagcgtgca
    20041 ggagctgcag cacatcggca gcgtggagat gcaggccggc gacatcttcg aaatccacac
    20101 accgggcggg ggcggctatg gtgcgcgctg atccatcatc acttccgaag tgcggtgagc
    20161 acgctgcgcg atcgtccttg cctggcacgc cgatcacggc accggcgggc gcgtccaact
    20221 gccgcttcta ggttcgacgg tcgttcacag gatcatcaac caaatcggcc cccggcgctt
    20281 gatggaactg cgccgcaagc tatcaaaatc gtagttatca agcgcaaaga aaacggcacg
    20341 cgctggcgtg ccgtccgagg ggtcagggcg cccggcgggc gcgtgggcgt cagggtttgc
    20401 tggcggccgt tgcgggtgcg ctgggggccg gggtcttggc cgcggtgttg ctgctcgctg
    20461 ccgccggcgc cgctttcggc ttggcggggc tggccgcggc cgctgcggtg taggcggcgc
    20521 cgttgaccgt caggccagcg gcagagaact tggcggcgtt cttctcgccc acgcctttga
    20581 cgcggctgat gaagtcggcc cagtccttga attcgctctt cttgcgttcc ttgatgatgc
    20641 ggctggacat gccggggccg atgcccttga cgctgtccag gtcggcggcg ctggcggtgt
    20701 tggcgtcgac cgcggcaaag gcgaaggcgg cgtagagcag gctcagaagg acgaggatct
    20761 tcttcaacat gggcgaacac tcctggtgac gaaagcatgc cggctgaggc cgccggcggg
    20821 ccggtattca actgatcggg cggtgtcggc gttgacacta ccacagccgc ggcgcgggtc
    20881 gcccagcggt cacagctcct cgatgcggcg cggcggaaag ctctcccagg cctggcagcc
    20941 cggacattgc cagaaatacc cctgcgcctc gaagccgcag gccgcgcagc ggtagcgcgc
    21001 cagaggccgg gcggcgtggt cgaccgagcg ctgcaccagg gcgtgggcgt cctgctgggc
    21061 aaagccttca tggctgagcc agcgcgccgc cgcgatcagc gacggctcct gggccatgtg
    21121 gcgcaggtag ccggagcgcg cttgcgcctg gggcacaccg gcggtgacgt cggcctgcac
    21181 gatggcgtcg gccacgtcga tcgatggcgt gctggcgtag ctgcgctgca gcagttcgcg
    21241 cacgcgcgtg gcctgtccac aggcctgtcc gatgcgcacc agttcgctgg ccaccagcgc
    21301 aatggcctgc ggcgcgtgct gctcgaggct ggccagctga tcgagtgcgt cggcggcatc
    21361 gccctcgcgc gccagcaggc cggccagctc gatgcgtggt cgggccgcat gcggggcctg
    21421 gtcgatcgcc aggcgcaggt attgctgggc ggcggtgcga tcgctcttct tgttcagcgc
    21481 ctcgctggcc tgctcgcatt ggtagtgtga caagcgcaag gtgaaatcgc ccgctccgcg
    21541 ctgctgcaac cgccgggcca gggccccggc ttgcggccag tcgcgcgccc gctcgtgcag
    21601 cgacagcagg gtcaggccgg cctgctcctc gaacgccgtg ccctcgagtt tgcgcagggc
    21661 gtcttcggcg cggtccagca gcccggcctt caggaagtcg agcgccagcg catgctgggc
    21721 acggtcgcgg tcggcctggc tcaggtcggc gcgcgacagc aggtgctcgt gcacgcgcac
    21781 ggcacgctcg tattcgccgc ggcggcgaaa caggttgccg agggcgaagt gcagttcgga
    21841 ggtgtcgggg tctttctgca ccgcctcgat gaaggcgtcg atggcctggt cctgctgctc
    21901 gttcagcaga tggttgaggc ccttgaagta ggccttgggc gcctgccggt tctcgatgcg
    21961 cagctggcgc aggtccaggc gcgaggcaaa ccagcccagg gcaaacacca ccggcaggcc
    22021 gaacaggatc cagccgagat caaactccat ggggaggctc cggcaccgaa ggctccgacg
    22081 gcgccggtac ggggccgggc atgggcgtgc ggccgtggcg gcgcgaccac caacccggca
    22141 gcatgcccag cacgcccacg atgaggccga tgccgaaggc caccagcacg atcagcgtca
    22201 tgggtgcacg ccactgatgg ccgaacagca ggtgcacggt ggcgccctgc tggttattga
    22261 gggcaaaggc gaacagggca aaaaaaacgg ctgccttgag tatccacgtc agcagccggg
    22321 caagtcggtt catgggtctt cttcggtgtc gaagaagatt ctagtgtggt gtttcccgat
    22381 cctccggtgc gccgcgtacg ccgggactga ccggctgcgc gcccacggct tctcgcagcg
    22441 ccttgcccgg cttgaagtgg ggcacgcgcc gctcggggat gtgcacgcgg tcgccggtgc
    22501 gtgggttgcg acccagacgg ggcggccggt ggttgatgga gaagctgccg aagccgcgga
    22561 tctcgatgcg atgaccgcgc gccagcgcgt cgctcatcgc atccaggatg gtcttgaccg
    22621 ccgcgtcggc gtcgcgctgc gtgagctggc tgaagcgttc ggccagcagt tcgaccagat
    22681 cggagcgtgt catggcggtg agcgcggcca gagggggcgc ccctggccgc gcggctcggc
    22741 ttacttgtcg ttgccgtcca gcttggcgcg cagcagggcg cccaggctgg tggtgccggc
    22801 gctgccgctg ctctggctca ggctggccat ggcttcctgc tggtcggcgg cgtccttggc
    22861 tttgaccgac agctggatgt tgcgcgactt gcggtcgacg ttgatgacca cggccgtgac
    22921 ttcgtcaccg accttcagca cctggctggc gtcttccacg cgatctcggc tgatctcgga
    22981 ggcacgcagg tagcccacca cgtcgttgcc caggtcgatc tcggcgccac gtgcgtccac
    23041 ggtcttgacg gtgccggtga cggtctggcc cttgtcgttg accgacacga aggtggtgaa
    23101 cgggtcctgg tcgagctgct tgatgcccag gctgatgcgc tcgcggtcga cgtcgacggc
    23161 cagcacgatg gcctcgactt cctggccctt cttgtagttg cgcacggcgg cttcgccggg
    23221 ctcgttccag gacaggtcgg acaggtgcac caggccgtcg atgccggcag ccaggcccac
    23281 gaacacgccg aagtcggtga tggacttgat ggggcccttg acgcggtcgc cgcggcgggt
    23341 gttctgcgcg aattcctgcc aggggttggc gcggcactgc ttcatgccca ggctgatgcg
    23401 gcgcttgtcc tcgtcgatct ccagcaccat gacctcgacc tcgtcgccca tcgacacgat
    23461 cttgctgggc gcgacgttct tgttggtcca gtccatttca gatacgtgca ccagaccttc
    23521 gatgcccggc tccagttcga cgaaggcgcc gtagtcggcg atgttggtga ccttgccgaa
    23581 catgcgtgtg ccctgcgggt agcggcggct gacgcccagc cacgggtcgt cgcccatttg
    23641 cttgaggccc aggctgacgc ggttcttttc ggtgtcgaac ttgaggatct tggcggtgat
    23701 ttcctgacca gcctgcacca cctcggaggg gtggcgcacg cggcgccagg ccatgtcggt
    23761 gatgtgcagc aggccgtcga tgccgcccag gtcgacgaag gcaccgtatt cggtgatgtt
    23821 cttgaccacg ccgttgacga tggcgccttc cttcagcgtc tccatcagct tggcgcgctc
    23881 ttcgcccatg gaagcctcga ccaccgcgcg gcggctcagc accacgttgt tgcgcttgcg
    23941 atcgagcttg atgaccttga actccatcgt cttgttttcg tacggcgtca ggtccttggt
    24001 cgggcgggtg tcgaccagcg agccgggcag gaaggcgcgg atgccgttga ccagcaccgt
    24061 caggccgccc ttgaccttgc cgctggtggt gccggtgacg aactcgcccg agtccagggc
    24121 tttttccagc gccagccagg aggccaggcg cttggcggtg tcgcggctca ggatggtgtc
    24181 gccgtagccg ttttcgatcg aaccgatggc caccggcacg aaggcgccgg gctccacctc
    24241 gacctcgccc tggtcgttct tgaattcctc gatcgggatg taggcctcgg acttcaggcc
    24301 ggcgttcacg acgacgaagt tgtgctcgac gcgcaccact tcggcggtga tgacttcacc
    24361 cgggcgcatc tcggtgcgct gcagggattc ttcaaacaac tgggcaaaag attcagacat
    24421 ggggtttcct tgttgcgcgc gcacgggttc tcacgacggc accatgcaat cgtttccaga
    24481 ggctgtgcgg cggcgggggt ggcggccaga gccgcgggtt gcgggttgaa caaccaccag
    24541 gcctggtgcg ctgagcaagc gcggcaggga ggccgggtgg accgaagaac gccagaaccg
    24601 ggggttctgg cgctgtggct gcgttctagg tcgagatgtc gatcagaacg gctgccgtgc
    24661 ctgccaccag ctcagcacct gttcgaccga ctgcgcaatc gaaagcaggg agttgtccag
    24721 cagcagggcg tctggcgcgg ccttcagtgg cgcggcggcg cggtctcgat cccgcgcgtc
    24781 gcgcgcctcc aggtcggcgc gaagactatc aatgttagca gaaattccct tggaaatcag
    24841 ctgcttatac cgcctttcgg cgcgctgggc ggcgtctgcg gtcagaaaca ccttgagccg
    24901 ggcgtctgga aacaccaccg ttcccatgtc gcgcccatcg gccaccagac ccggcaaccg
    24961 ggcaaagtcg cgctgcaggg ccagcagcgc ctggcgcacc gcgggcaggg ccgaaacgcg
    25021 cgaggcgtcc atgcccgccg cttccgtgcg aatggcgtcg gtgacgtcct ggtcggccag
    25081 ccagaccgtc tgggcctcga aacgcaccgg caactggcgc gcgaggctgg caagggcggc
    25141 ctcgttggtg gcgtccggtg ccatgccctg ttggcgcgcg gccagaccga ccagccggta
    25201 cagtgcgccg gaatccagaa accggaaccc cagccggcgg gccagttcgg ccgccagcgt
    25261 gcctttgccg gaggccgtgg gtccgtcgat gcagatcacc ggcaccgggc cggccgcacc
    25321 tgcgagggcg aacagcgtct cgaagtagtc gggaaaggtc ttgccgacgc aacgcgggtc
    25381 ttcgatgcgc accggcagcc cagcggggtt gaacgccgcc agcgacccac acatggccat
    25441 gcggtggtcg tcgtaggtgt ggatcgacgc cgcccgccac gccgcggcac cggcaggtgg
    25501 gtggatgcgg atgaagtcgg caccttcttc cacctgggcg ccgagcttgc gcagctcgtt
    25561 ggccatggcg gcgatgcggt cggtttcttt gacgcgccag ctggcgatgt tgcgcaaggt
    25621 gctggggccg tcggcgtaca gcgccatcac cgccagcgtc atggcggcgt cgggaatgtg
    25681 gttgccgtcc agatcgatgg cgcgcagcgg ccaggcgccg cgccgcacgc gcagccagtt
    25741 ggggccgctt tcgatctgcg cgcccatctg gcgcgcggcc tcgacgaagc ggatgtcgcc
    25801 ctggatggac gcttcgccaa ccccttcgat cgtcaagcca ttctggcctg cagcgccctc
    25861 tgacaaagcg cccgcagcta tgaaataact ggcagacgag gcatcggcct cgacgtggat
    25921 ttcgcccggt gatcggtagc ggctgccgct ggggatgacg aagcgctgcc agccctggcg
    25981 ctgcacggtg acgccaaagc gcgccagcag ctgcagcgtg atgtcgatgt agggcttgga
    26041 gatcagctcg ccgaccacgt cgatgacgat gtcgcgcgcg ccggggcggc cggccgccag
    26101 cggcagtgcc agcagcagcg cggtgaggaa ctggctggag acgtcgccgc gcacggggat
    26161 gggccgatcc aggctgaggt ccggtgcgcc gatgcgcagc ggcgggtagc cggcctggcc
    26221 aaggtagtcg atgcggcagc cgagcgtgcg cagcgcatcg accaggtcgc cgatcgggcg
    26281 ctcgtgcatg cgcgggatgc cgctgagtcg gtagtcgccg cccagcacgg ccagcgctgc
    26341 ggtgagtggc cgcatggcgg tgccggcatt gcccaggaac agttcggcct gcggctggcg
    26401 cggccggctg cccaggcctt cgatgacgtg cgaggtgccc tgcagttcaa ccacgcagcc
    26461 gagctggcgc agcgcctgca gcatcacgcg ggtgtcgtcg gagtccagca ggtcgtgaat
    26521 gacggtccgc ccttcgcaca gagcggccag cagcaacacg cggttggaga tgcttttgga
    26581 gcccggcaga cgcaccgtgc cgtgcaccgc gcgcagggcc gggatgtcga gaaacggaag
    26641 atcgaacacg gtgcggccgg cgcgctcagc gggcacctga cgacaggcgc cagttggcgc
    26701 gcgcatggct ggcgcggttg atcaggcgtt ccagatcgtc ggcctggccg ttggcgatca
    26761 gtcgctgcag gtcgttgagc gcatgctgaa acagttcggt ctggcgcccc agctcttcgc
    26821 gattggcgat cagcacgtcg cgccacattt gcgggtcgct ggcggcgatg cggctgaagt
    26881 cgcgaaaccc cgggccggcc agcgacaggt attcgcgccc ttcgggttgg ttcacgatgc
    26941 tgttcatgag cgcgaaggcg accagatgcg gcaagtgact gactgccgcg aaggcggcgt
    27001 cgtgcgattc gggcgacatg ttgaccacct gacaaccgag cgcttgccac agggcgttgg
    27061 cctgctccag cttggccatc agcgtgcgct cgatgggggt cagaatgacc tgcttgccag
    27121 cgtacagccc ggcgtcggcg tgctccaccc ccgacacctc gcgcccggcg atcgggtggc
    27181 agggcacgaa cacgcccacg tcctgcttca gcacgcgccg cgcggcgtcc accacgtcgc
    27241 gcttgctgga gccgacgtcc atgatcagca tgtcgccgtt gaccaggtgg cggatggcct
    27301 tgagcgtggt ttcggtggcg gccaccggca ctgccagcag caccaggtcg gcgccggaca
    27361 ccgccagcag ggccgaaggt gcctcgacgt cgatcacgcc cagttgcagc gcccgcgccg
    27421 tggtggaggg cgatttgctg taacccacca cgcgcttgac caggccggcg cgtttgagcg
    27481 ccagggcgaa ggagccgccc atcaggccgc agccgatcag tccgagttgc tcaaacatct
    27541 tgacccaggg gataggcgcc cagcatcttg tagaaggcgc agtaggcctg cagttcgctc
    27601 agcgccgctg ccacattggg ctcgccggga tgaccttgca ggtcgatgta gaagaagtat
    27661 tcccacttgc ccgatttggc cgggcgcgac tcgaagcgcg tcatgctcac cccatgcttc
    27721 ttcagcggtg tcagcatgtc gtgcaccgag cccggtttgt tgggcaccga caccacgatg
    27781 ctggtgcagt cgcgacccgt ggccgccggc gtgcccagcg tctgcggcag gcagatgatg
    27841 gcaaagcgcg tgcggttgta ggcctcgtcc tggatcgcat gggcgacgat gtgcagcccg
    27901 aattgagcgg cggcacgctc actggccaga gccgcccagg ccgggttggt ggccgccagg
    27961 cgcgcacctt cggcgttgct ggagacggcc cggcgctcgg cgcccggcag gtgctcgctg
    28021 agccagccct ggcattgcat cagcgcctgc gggtgcgcca gcaccacctc gatcttgtcc
    28081 agcgcgttga cctgccgcag caagttgtgc cgcaccagca ggctgacttc gccgatcaca
    28141 tggaccggcg aatgcaggaa caggtcgaac gagcgcgcga ccacgccctc ggtggtgttt
    28201 tccatgccta ccacgccaaa ctgggccgtg ccggccgcgg tggcgtgaaa cacctcgtcg
    28261 aagctgctgc agtagatcag gttggcggca ctgccgaagt actcgatcgc cgcctgctcg
    28321 cagaacgtcc cctgcggccc caggacagcc acgcgctgcg gcacttccag cgccaggcag
    28381 gccgacatga tttcgcgcca gatcgccgcc acatggctgt tctgcagcgg cccggtgttg
    28441 ctggcctgga tcttgtcgat cacctgcgcc acgcggtcgg ggcgaaagaa cggcgtgcct
    28501 tcgtggcgct tgagctcgcc cacctgctcg gccactttgg cgcgctggtt gagcagcgtc
    28561 agcaactgcg catccaggct gtcgatctgc gtgcgcagct gggccagggc ttcggactgg
    28621 acgggcgtag ttgagctcat gcggtgacgg gatggcctgg ggcgggccgc agcgcctcgg
    28681 acgggcgcct gtcggcacgt gggtggctgg ggtcgggacc ttatttcttg gtggccgcag
    28741 cggcgccgga tgcgccgccc gccgccgctt tcacgatccg atcggccgcg ctctcgaagg
    28801 tcttggcgct ggcttccacc tggctgcgcg tgctgtcgat gatgcgcttg gcccaggcgt
    28861 cggtggcgtc tgctcccagc gcctgaagct tggcgctggc gggcgattcc atgtaggcga
    28921 tgatggtctt catctcgtct tcggagagtt tctccatgaa gatcggcacc atcgccgctt
    28981 cggccgcttt gcccacctgc gcttcgatcg acttgtgggt gctgtcggcg aacttcttca
    29041 gctcgacgtc gagtttgtcg cgcacctcct tctggcgggc cggcggcacg gtctcatcga
    29101 gtcgctgcga ccagccggcc acgatgggct gcaccgcact gttggtgagc tgatcggtga
    29161 tggccggacc atcggtcttg agctgcagct gcaccagctt gttggccagc gcacgtttcg
    29221 catcattttg ggccatggcg cccgtggata gtgcgcaagc agctacgaaa acaatagctg
    29281 tcgggaggcg ggaaatggtc aaattcactg ggttcactcc gttggggatt cctgggtggg
    29341 gtcgatggcg ggatcctgcg cgtcgttttc gacgatgcgc tgcagaccgc tcaactgggc
    29401 gccgtcgtcc agcgcgatga gcgtgacacc ttgggtggcg cggcccattt cgcggatttc
    29461 ggcgacacgg gtgcgcacca gcacgccctt gtcggtgatg agcatgatct cgtcgtcgct
    29521 gctcacgagc gtggcggcaa ccaccttgcc gttgcgctcg ctctgctgga tcgcgatcat
    29581 gcccttggtg ccacgaccgt ggcgtgtgta ttcgttgata ttggtgcgct tgccgtaacc
    29641 gttttcggtt gcggttaaga cactgttgcg cgcattcggt tccgcgctgt cggtggtggt
    29701 ttcaccgtcg gccaccagca tggcgatgac gcgctggcct tcttccagcg acataccgcg
    29761 cacgccgcgc gcgttgcggc ccatcgggcg cacgtcgttt tcgtcgaagc gcaccgcctt
    29821 gccgccgtcg gagaacagca tcacgtcgtg cgcaccatcg gtcaaggcgg cgccgatcag
    29881 gtagtcgccc tcgtccaggt cgacggcgat gatgccggcc ttgcgtgggt tgctgaactc
    29941 gggcagggcg gttttcttga ccgtgcccat ggaggtggcc atgaacacga agtgatcgtc
    30001 cgggaaattg cgcattgcgc cggtgagcgc cagcaccacg ttgatcttct cgccctcggc
    30061 cagcgggaac atgttgacga tggggcgccc gcgcgagccg cgcgaaccgg acggcacttc
    30121 ccagaccttg agccagtaca ggcggccgcg gttggaaaag cacaggatcc agtcgtgcgt
    30181 gttggcgatg aacagctggt cgatccagtc gtcttccttg gtcgccgtgg cctgcttgcc
    30241 gcgaccgcca cgcttctggg cccggtattc cgataccggc tgcgccttga tgtagccggt
    30301 gtggctgagc gtgaccacca tgtcggtggg cgtgatcagg tcctcggtgg ccagatcctg
    30361 ggcgttgtgt tcgaccgtcg aacggcgcgc gccgaccttg gtctggccaa attcggcctt
    30421 cagtgccgac agctcctcgc caatgatggt ggacacgcgc tcggggcgcg ccaggatgtc
    30481 cagcagatcg tcgatctggg ccatcacttc cttgtattcg gcaatgatct tgtcctgctc
    30541 caggccggtc aggcgctgca ggcgcatctg caggatttcc tgcgcctgag tgtcggacag
    30601 gcggtacagg ccatcggcct gcatgccgca ggcgcgctcc agaccctcgg ggcggtagtc
    30661 gtcggcgttg accacgccgc cgtctgcgcg cgtgcgcgtc agcatctcgc gcacgagctg
    30721 gctgtcccag ctgcgcgcca tcagctcggc cttggccacg ggcggcgtgg gcgattcgcg
    30781 gatgatgcgg atgaactcgt cgatgttggc cagcgccacg gccaggcctt ccagcacatg
    30841 gccgcgctcg cgcgccttgc gcaggttgaa cacggtgcgc cgggtcacta cctcgcggcg
    30901 gtgctgcagg aagacctcga ccagctcctt cagattgcac agccggggct ggccgtcgat
    30961 cagcgccacc atgttcatgc cgaaggtgtc ctgcagctgg gtctgcttgt acaggttgtt
    31021 gagtaccacc tcgggcactt cgccgcgctt gagctcgatc accaggcgca tgccggattt
    31081 gtcggactcg tcctggatgt ggctgatgcc ctcgagtttt ttctcgtgca ccagctcggc
    31141 catgcgctcc tgcagcgtct tcttgttgac ctggtagggc agctcgtcga cgatgatggc
    31201 ctggcgctgg ccgcggtcga tgtcctcgaa gtgcaccttg gcgcgcatca ccacgcgccc
    31261 acgcccggtg cggtagccct cgcgcacgcc ctgcatgccg tagatgatgc cgccggtcgg
    31321 gaagtccggt gccggcatga tctccatcaa ttcctcgatg ctggccgccg gctgacgcaa
    31381 caggtgcaga caggcgtcca ccacttcgtt caggttgtgt ggcgggatgt tggtcgccat
    31441 gcccacggca atgccgcccg agccgttgac cagcagattc ggcaggcggc tgggcagcac
    31501 cagtggctcc tgctcgctgc cgtcgtagtt ggggccgaag tccaccgtct ccttgtcgat
    31561 gtcggccagc atctcgtggg cgatcttcga caggcggatt tcggtgtaac gcatggcggc
    31621 ggcgttgtcg ccatccacgc tgccgaagtt gccctggcca tcgaccagca tgtggcgcag
    31681 cgagaagtcc tgtgccatgc gcacgatggt gtcgtacacc gcgctgtcgc cgtgcgggtg
    31741 gtacttgccg atcacatcac ccacgatgcg cgccgacttc ttgtacggcc ggttccagtc
    31801 gttgttcagc tcgtgcatgg cgtacagcac gcgccggtgc acgggcttca ggccatcgcg
    31861 cgcgtcgggc agggcgcggc ccacgatcac gctcatggcg taatcgagat agcttcgccg
    31921 catctcctct tccagactga tggggagggt ttctttggcg aatgaagtca tagaccgagc
    31981 aacaaggaga gcaacggggc gggatccggc gggcgaaagc gatcgattct actaggcgcc
    32041 cggacttgtg gttccatagc aacacttgat cgaaattgag cctctgtctg aggcgctgag
    32101 gcgccgttcc accaagatcg cgctatcgcc tgtggcacaa tcgcctcaac gttttcggtg
    32161 gtggtcactt gaagcgtccg atttatcgca gcccggctgc ggcttttttc ccatgaggag
    32221 aaccatgaag aaactcaaca aagtagcggc tctgttcgca gtggcaaccg tggctggttc
    32281 cgccttcgca gctggttcga cgatcaattc cgacaactgg cgcaatggcc acggcaacct
    32341 gatctggaag aacggcacca acgaactgtg ctggcgcgat gcgggctgga cgccggctac
    32401 cgctgctcct ggctgcgacg gcgcgctcga ggcgcccaag gctgctgcgc cggccgcggc
    32461 accggcaccg gctgctgccg ctccggcacc tgctccggcc aaggcacctg ctccggcgcc
    32521 tgccctggct gcccagaagg tcacctacgc cgccgacgcc ttcttcgact tcgacaagtc
    32581 ggtgctcaag ccggaaggcc gtgccaagct ggatgacctg gtcagcaaga tccagggcat
    32641 caacctggaa gtgatcattg ccgtgggcca caccgactcg atcggttccg acgcctacaa
    32701 ccagcgactg tcggtgcgtc gcgccgaggc cgtgaaagcc tatctggtgt ccaagggcat
    32761 cgaacgcaac cgcgtctaca ccgaaggcaa gggtgagaag cagccggtgg ccgacaaccg
    32821 caccgcccag ggccgcgcca agaaccgccg cgtcgaggtg gaagtggtcg gcacgcgcgc
    32881 cgcgaagtaa tcggtccagc ttcatcactg gattgcatca ggccttgggg gcgaaagctc
    32941 ccagggcctt tttgtttgac
//
LOCUS       DAOQTZ010000016        32283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1612_length_32283_cov_12.641399, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000016 DAOQTZ010000000
VERSION     DAOQTZ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 32283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32283
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1612_length_32283_cov_12.641399"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /gene="xth"
                     /locus_tag="PKN51_03430"
     CDS             <1..777
                     /gene="xth"
                     /locus_tag="PKN51_03430"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNT84182.1"
                     /translation="ISTWNVNSLTARLPHVLDWLAANPVDVLALQELKLTDDKFPHQA
                     LGEAGYAAVAFGQKTYNGVALLTRAPLAAQAVVRNIPGFADESARVIAATLEAPGGPL
                     RVVNGYFVNGQAPGTDKFDYKMSWLRALRDWLREELAAHPRLVLLGDFNIAPEDRDSY
                     DPVGLAETIHHTSEERHHFQALLGLGLADAFRLFEQPDKSFSWWDYRMLGYQKNRGLR
                     IDHILISQPLVAQATACHIDRVPRKWPKPSDHAPVTLELA"
     gene            complement(1214..1738)
                     /locus_tag="PKN51_03435"
     CDS             complement(1214..1738)
                     /locus_tag="PKN51_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84183.1"
                     /translation="MKPLLPIPWVPLLAAALAVAAWPAAAQDRIYRCGNEYTNQPKGR
                     SDCKLVEGGNVTIVRGTAPAAAPAATAPAGTAPAARSGGASPASAPRVDNAEQRKRDA
                     DAKAILQQELRRAEAKQAELEKEYKGGEPDKIGGEARNHQKYLDRVAELKAAIERNQS
                     DIEGIKRELGRLGP"
     gene            complement(1905..3320)
                     /gene="glnA"
                     /locus_tag="PKN51_03440"
     CDS             complement(1905..3320)
                     /gene="glnA"
                     /locus_tag="PKN51_03440"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNT84184.1"
                     /translation="MTRSVADVMKLLQEAECKFVDLRFTDTRGKEQHVTVPVTAFDED
                     KFSDGHPFDSSSVAGWKGIEASDMLLMPDPNTAYVDPFFEEPALVLTCDVLEPGDGKA
                     YERDPRSIARRAEAYLKASGIGDTAYFGPEPEFFVFDSVRWGTEPGHSFYRIEEYEAP
                     WNTGAELEGGNRGHRPRTKGGYFPVPPVDSTHDMRAEMALILDALGVPVEVFHHETAG
                     AGQMEIGTRFSTLVERADWTQRLKYVVWNVADRYGKTATFMPKPYHGDNGSGMHVHQS
                     IWKGGKNLFAGKGYAGLSETALFYIGGIIRHARALNAITNPTTNSYKRLVPHFEAPVK
                     LAYSARNRSASIRIPHAASDKARRIEARFPDPMANPYLCFAALLMAGLDGIENKIHPG
                     EAATKDLYHLPPEEDKLVPTVCHSLDQALEELDRDRAFLTRGGVFTDAMLDAYIELKM
                     TEVNRIRVQVNPAEYDLYFSL"
     gene            complement(3483..4298)
                     /locus_tag="PKN51_03445"
     CDS             complement(3483..4298)
                     /locus_tag="PKN51_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNT84185.1"
                     /translation="MPEFGLIVVGDEILSGKRADKHLPKFVELLTERGLRLAWAEYVG
                     DEPARITATLRRALATGDVVVCTGGIGATPDDHTRQCAAAALGVPLALHPEARRLIEQ
                     RGQDMARQQGQPWEPERADNRQRLNMGVFPAGARIVPNPYNQIPGFALAGPAGGAIYF
                     VPGFPVMAWPMMAWALDTEWSHCFTRGAWRERSVVLYGAMEAPLTPVMERVEAAHPGV
                     RVFSLPSVDHPEHGRHIELGVKGAPAQADAAYADLRAWLQPFALSYGPELVRG"
     gene            complement(4305..5198)
                     /locus_tag="PKN51_03450"
     CDS             complement(4305..5198)
                     /locus_tag="PKN51_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNT84186.1"
                     /translation="MLPRVFDAFLRALGHVFLPRVIGMTLLPLLVMSVVALLLAWLGW
                     QAAVAWMQAALQGWSPLGWAEGWLNIQRPGGLALLLAPLFVVLLATPVIVLASLVVVS
                     LLLTPALTRLVAERRFANLARLGGGSLAASLAWSTGSLLLAVLALVLTLPLWLIPPLV
                     LVLPPLIWGWLTYRVMSFDALVDHASAEERRTILRQHRWPLLLIGVICGYLGAAPGIV
                     WASMALFAVLFVLLVPLAIWIYTFVFVFSSLWFAHYALDALQRLRDQRPPGTAPAAAL
                     PAAQAPDRVTDLPWRDLNGSA"
     gene            complement(5229..6266)
                     /locus_tag="PKN51_03455"
     CDS             complement(5229..6266)
                     /locus_tag="PKN51_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNT84187.1"
                     /translation="MTPADLFASAQQGLFETLIEPLAFALGLGNLLEDGFVAAGWLLI
                     GVLQIGVLLAVIGPLQRLWPAEPVRDRAAIRVDVLYTLLHRLGLFKLAMFFSVEGWLT
                     DAIGVLRARGLPTLHLDALWPGVTDRPLVSFVLYLLVFDFIGYWVHRAQHQWDWWWKL
                     HAVHHSQRQMTMWSDDRNHLLDSVLTDAIMVVVAILIGVAPSQFVLLVACTQLLESLQ
                     HANLRWSFGRVGERLLVSPRFHRRHHAIGIGHEARAAREPGAAGPSQGASAPLGGSAP
                     RAAGSVGAHVRGVNFGVLLPCWDALLGSADFSGRCEPTGIRDQVEQGRDYGRGFWAQQ
                     WRGLLRLAGRA"
     gene            complement(6281..6988)
                     /locus_tag="PKN51_03460"
     CDS             complement(6281..6988)
                     /locus_tag="PKN51_03460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT84188.1"
                     /translation="MYLSFDTGHMGVAALVAEVLRRQQVRVTFFAANERTQEGDGSLS
                     RHWGPWWKARAAEGHELASHTWDHVYWRADLPGREPRFRVRPSAGPLAGREFTWSAEQ
                     YCQNIEQASERLAYYSGRKPLPLFRAPGGKISPRLLAAARACGYEHVGWSPAGFLGDE
                     LPSDKYPNRLLLERALKTIQPGDILLAHLGIWSRQDPWAPAVLEPLIEGLKARGFVFR
                     TLREHPQYAPWIAQQRA"
     gene            complement(7095..8051)
                     /locus_tag="PKN51_03465"
     CDS             complement(7095..8051)
                     /locus_tag="PKN51_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNT84189.1"
                     /translation="MGAAALAPGALAAPTAAPVLVLNSLDGTVSVIDGRRWVELVRIP
                     TGKEPHHLYMTPDERSVIVANAGSDSLTFIDPRTAIVQRTLRDILDPYHLRFSPDMRW
                     FVTAANRLNHIDLYHWDGQQPHLARRIPTGRTPSHLWIDARSTTVWATMQDSHELVAI
                     DLASQQLMHRVLTGPLPADVFVTPDERHLLLGLTGGSGVEVFELAAGQAPRRVGLVAT
                     GKGAHAFRALGDRRHVLVSNRVAGSISQIDTTRLAVVAEFPAPAGPDCMDVSADGSLI
                     MVGSRWAGKLSLIDVAQRKLVHQVKVGKSPHGVWTLDHAPRV"
     gene            8289..9077
                     /locus_tag="PKN51_03470"
     CDS             8289..9077
                     /locus_tag="PKN51_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT84190.1"
                     /translation="MPAMRSVDIDGVSLELQHIAPPAGAAPARAPLVFLHEGLGSVAL
                     WRDWPARLCAATGRAGWVYSRRGYGRSSPVPDVRGAGRLPPDYLHREALDVLPALLRA
                     LGLARPVLVGHSDGASIALIHAAHHPVTACVAMAPHVMVEALCLRAIEQTRQHFETGA
                     LRIRLQRFHDDVDGAFWQWNDAWLNPQFHGFDIRADIRSIDAPLLAIQGVDDGYASMA
                     QLDDIATALPHTRLLKLPACGHAPHQDQPAAVQQAITDLLADLP"
     gene            complement(9138..9866)
                     /locus_tag="PKN51_03475"
     CDS             complement(9138..9866)
                     /locus_tag="PKN51_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT84191.1"
                     /translation="MRLSELLFSQGFGTRRVCAGLVEQGLVRLGERRCTDPSAEVDLS
                     AGPLRYEVQGQAWTYHPRAYLMLHKPAGFECSHRPGRWPSVYTLLPAPLRQRPTAGKV
                     AGVQAVGRLDQDTTGLLLLSDDGAFIHRMNAPRRHVPKVYEVGTRHPLDERQLERLRS
                     GVVLDDDPRPVVAAAAEASGERQLRLTLTEGKYHQVKRMLAAVGNRVETLHRSRIGGL
                     SLPAELAPGQWRWLEPSELDLLRP"
     gene            10038..10781
                     /locus_tag="PKN51_03480"
     CDS             10038..10781
                     /locus_tag="PKN51_03480"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNT84192.1"
                     /translation="MSAISGSTTFWAIVLAIVGLFTGYLGLSSFMPRGRDGQAGKPRS
                     KLRMPQQWPLVPRPLANSRERLVWHWLRKVFPGHHIMVKLPVTRFTMPRQPGEASEWF
                     ELLSRAYCSFTLCDSKGHVIGCVDVLGPHGLSRSNRQLKQTLLAQCGIAYWVISPDAL
                     PEPSALRADFLGVNPADLEAMPASSFAELEAARHHLHQTLDRNRSQRAPGPAPDPTPA
                     HEGDAPSGWAQADSFLGSLDSRRTPLDRH"
     gene            complement(10903..11496)
                     /gene="orn"
                     /locus_tag="PKN51_03485"
     CDS             complement(10903..11496)
                     /gene="orn"
                     /locus_tag="PKN51_03485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNT84193.1"
                     /translation="MTDTVCTPQDTAPALARSDLNLVWLDCEMTGLDPEKERLIEIAV
                     IVTGPQLEPRIEGPVLVIHQPDSVLNAMDAWNKGTHGRSGLIDKVKASTLTEAQAEAQ
                     LIEFLSRYVSKGVSPLCGNSIGQDRRFLVRYMPRLEAFFHYRNVDVSTLKELARRWRP
                     ELVEAFRKQQSHTALADVHESIDELEHYREHFIRLAT"
     gene            11604..12911
                     /locus_tag="PKN51_03490"
     CDS             11604..12911
                     /locus_tag="PKN51_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNT84194.1"
                     /translation="MLNPTPSPALLTTLVFSLALLLYVAVKFWLASRQMRAVAAHRAA
                     VPAEFAGTIGLAAHQRAADYTIAKLRFGLLELLLAAAVLLGWTLLGGLDALNGALLGA
                     VGPRPLAQPLALLVVFALINGAIDIPGALYHTFVIEQRFGFNTMSWRLWLIDLLKTAL
                     VSTVIGVPLAALVLWLMQATGTLWWLWTWGAWMGFSLLMLVLYPTVIAPLFNRFEPLR
                     DDALTERVTRLMARCGFAAQGLFVMDGSRRSAHANAYFTGFGAAKRVVFFDTLLQQLS
                     PPEVEAVLAHELGHFRHRHIVKRIAVLFAVSLAGLALLGWLAEQPWFYSGLGVTPNLP
                     PQMGGAVPNHALALLLFMLVVPVFSFFLSPLMAALSRRHEFEADAYAVRQASAQDLGN
                     ALVKLYKDNASTLTPDPLYVRFYYSHPPASERIARLGARVLPV"
     gene            13030..14004
                     /gene="rsgA"
                     /locus_tag="PKN51_03495"
     CDS             13030..14004
                     /gene="rsgA"
                     /locus_tag="PKN51_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNT84195.1"
                     /translation="MSRARPPSAPRTAAPALHDGLVVASHGRHCVVELPAGERVICHP
                     RGKKLGVVVGDRVRWRPSHDEGTVEQIAPRRNLLYRQDAVRTKSFAANLDQVLILLAA
                     EPEYSEHQLARALIACEAADIEPLIALNKADLAAPFERAWQRLAPYRAMGYAVLPLAA
                     RPQGGAAPADDGLAALAQRLQARTTLVLGPSGAGKSTLINRLVPGAAAQTGEISQALG
                     SGRHTTTTTTWYWLDAARRSALIDSPGFQEFGLHHIDPMQLAAYMPDLRPHVAACRFY
                     NCTHLHEPGCGVRAAQGFDTESGSGAQGISASRYKIYSDLFAELRSGA"
     gene            complement(14024..14680)
                     /locus_tag="PKN51_03500"
     CDS             complement(14024..14680)
                     /locus_tag="PKN51_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84196.1"
                     /translation="MNAWRGLHSAIFVIASSALWACAGGQKVPDWNIQAVGHVERFAE
                     AALRGDARVEAREFELARAEVARTGRSELVARVELTRCATRVASLELAPCAGFEPLRA
                     DAGEPERAYADYLAGQAAAAQASLLPPPHRAIAAGGDAVAAIDDPLARLIAAGVLLRQ
                     GRASPAVVAQAVDTASQQGWRRPLLAWLGVQQRLAQQAGRPEDAARLRRRMDLVAPPR
                     "
     gene            complement(14677..15702)
                     /locus_tag="PKN51_03505"
     CDS             complement(14677..15702)
                     /locus_tag="PKN51_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNT84197.1"
                     /translation="MTSTEPSAAPADAPDAVAQVARVPLRSLERRAVLLLLFMALLVG
                     AAVLYLMWVRGAFEETQTLYLSADDSEGVVVGMDMSFSGFPIGRVSRIELADQGTVRI
                     HVDVPVKSARWLRTSSVFTLEKGLVGAARLRAFSGILDDPPLPPGAERIVLRGDVSAE
                     IPKMVADARDVLLNLNRLTGEESALYATLGEVQRFTQRLNSSQGGLMAAVTGNEADAR
                     RVSELLGQTTQLVRRLDAVAQRVDSVVQRADRQVLGQDGLMTDAQVAVRQLGALLQEL
                     RQSMVKVDAVLTEVQGVAANAREASTDLGALRADVESSLRKVDALITELNRKWPFAPR
                     QQEVRLP"
     gene            complement(15709..16512)
                     /locus_tag="PKN51_03510"
     CDS             complement(15709..16512)
                     /locus_tag="PKN51_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT84198.1"
                     /translation="MSAGFSLYGWLAGLGQGLLRWLLGWWTVLFTGAQVAVLAFSPSS
                     YGPEARSAVLRHIWQAAGPGLPGFTTLMALFNVVLIRILVVTAFSYGLTQYALDAVVR
                     VLVLELIPLIAALFVAVEYTIPGGSELYKLRRGGALEALRARGCEPLSHEVLPRVLAG
                     MFAVLLLALLSCLISLLLAYVAVHGFTLAGLPSYNHAVGTIFNPVVSMIFSMKTLLFA
                     LAVALLPVANALRDIPHRGSRTSVELQGLVQMSVLMLVIEVASLIGNYY"
     gene            16714..18204
                     /gene="phaZ"
                     /locus_tag="PKN51_03515"
     CDS             16714..18204
                     /gene="phaZ"
                     /locus_tag="PKN51_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HNT84199.1"
                     /translation="MLYQIYEAQRSLLEPFADMADAASKLYGNRHTLLGQMPMAQRVS
                     AGFALFHRLGKDYEKPEFGIRTVDVDGVSVAIDERIEIDKPFCELRRFKRFSDDPPTL
                     DKLKRQPPVLIVAPLSGHYATLLRDTVRTMLEGHKVYITDWKNARMVPLSEGEFHLDD
                     YVNYVQEFIRHLQRNYGNCHVISVCQPTVPTLAAVSLMASRGEKTPITMTMMGGPIDA
                     RKSPTAVDDLAVKRSFEWFENNVIYRVPPNYPGAGRRVYPGFLQHAGFIAMNPDRHAS
                     SHYDYFRDLIKGDLSSADTHRKFYDEYNAVLDMDAPYYLETIQTVFQDFKLVNGTWDV
                     RSPEGKLERVRPQDITATCLLTVEGELDDISGVGQTKAAHRLCKGIARREGDHMEVEG
                     AGHYGIFSGRRWRTVVYPQIKAFIGEFHALSGLPPVFAPACSPASQPEPAPTQRKLNG
                     QRPPAKAPRQAGQGAAVPPAPRPRAAALPAASATAAGRRGKSARVG"
     gene            18271..18954
                     /gene="rsxB"
                     /locus_tag="PKN51_03520"
     CDS             18271..18954
                     /gene="rsxB"
                     /locus_tag="PKN51_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HNT84200.1"
                     /translation="MSAPPAPADDLAARLLDALPQTQCTRCGFPDCAAYARAMARGEA
                     PVNRCPPGGAEGVARLAALSAQPVQALDPSCGSEGPRGVAWIDEDWCIGCTLCIQACP
                     VDCILGSHKRMHTVIEAECTGCELCVPACPVDCIHMDNVSGSRTGWAAWSAQQAQAAR
                     KRYEFTSSRRRQDKGKSRISSGSSSTAPDGAPALPPAAPAGEAAEAERKRRLIEAALA
                     RARQQRPPR"
     gene            complement(18975..20030)
                     /locus_tag="PKN51_03525"
     CDS             complement(18975..20030)
                     /locus_tag="PKN51_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNT84201.1"
                     /translation="MTYIAKPRLHHPSLTRNKVGYTRRDYEGKVSTLCAGCGHDSVSA
                     AIVQACWELDIEPHRVAKLSGIGCSSKTPDYFLGASHGFNTVHGRMPSVLTGANLANR
                     DLLYLGISGDGDSASIGLGQFAHAMRRNVNMVYIVENNGVYGLTKGQFSATADKGSKS
                     KKGVVNADSPVDLVGLAMQLGASFVARSFSGDKAQLVPLIKAAIGHGGAAFIDVISPC
                     VTFNNHAGSTRSYDWVREHNEAVSRIDFITGREAITATIAPGQTVEVEQHDGSVLRLH
                     KLHADYDPTDRVAAMAYMQAHAARGELVTGLLYLEGEADDLHAAQHTSARPLNSLDVA
                     DLCPGAAVLERINAALR"
     gene            complement(20027..21856)
                     /locus_tag="PKN51_03530"
     CDS             complement(20027..21856)
                     /locus_tag="PKN51_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNT84202.1"
                     /translation="MKQIAAINDFVIKFANVNGSGSASANELFAKAILRMGVPVSPRN
                     IFPSNIQGLPTWYEVRVSEKGYLGRRGGIDMMVAMNPQTWAADVEEIAPGGYLLYDST
                     RPRAPSELRDDITVIGMPLTEICNATYSDPRQRQLFKNIMYVGALSVLLDMDAQVIEQ
                     LLGEQYKGKEKLIAANVQALNLGRDFAREHLRHPLPVRVERAHRVGQRIFIDGNTAAG
                     LGAVYGGATVCAWYPITPSTSVVEAFMSYCAKLRRDAEGRHNYAIVQAEDELASIGMV
                     VGAGWNGARAFTATSGPGISLMTEFIGLAYFAEIPVTLINVQRGGPSTGMPTRTQQAD
                     ILACAYASHGDTKHVLLLPEDPRECFEHTACALDLADRLQTPVFVMTDLDIGMNQRLC
                     EPLVWDDARRYDRGKVMSAEDLEQKDFGRYKDVDGDGIPWRTLPGTHPTRGSYFTRGT
                     TRDAYARYSERGPDYIYNVQRLLKKFATAATLVPQPVPRPAARRTRLGAIFYGSTSPA
                     MHEALDELATQGIHINALRLRAFPFPDTVKAFIDAHETVYVVEQNRDAQMRSLLINEL
                     EINPAKLRRVLHYDGTPITARFITQAITDHVHEAVVAPQENPT"
     gene            complement(21853..23685)
                     /locus_tag="PKN51_03535"
     CDS             complement(21853..23685)
                     /locus_tag="PKN51_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT84203.1"
                     /translation="MQPTDTRDPAYYHKVVDCQWACPAHTPVPEYLRLIAQGRYADSY
                     MVNWVANVFPGILGRTCDRPCEPACRRGRVEQAQTGKPEPVAICRLKRVAADFKDDVL
                     PRMPTPVSANGRRVACVGAGPASLTVARDLATLGYAVTVFEGEAKAGGFMHQQIPRFR
                     LPESVIDEETGYIFRLGVQFRAGQRIDSMKALLSEGYDAIFVGTGAPRGRDLDLPGRR
                     EAAAHIHIGIDWLASVSFGHVTSVGRRVIVLGGGNTAMDCCRSARRLGGEDVKVIVRS
                     GFDEMKASPWEKEDAQHEGIPIINMHVPKAFVHEGGRLTGMTFEIVRAEYDASGRRSL
                     VPTGQPDAFFACDEVLVAVGQENAFPWIERDLGIEFDRWGLPVLGQNTFQSTLARVFF
                     GGDAAFGPKNIITAVAQGHEAALSIDLFLDGQDVTRRPGPRANLVSQKMGIHEWSYDN
                     DPSNDQRYKVPWAKAEQALASIRVEVELGYDPRTAFKEASRCLNCDVQTVFSAPLCIE
                     CDGCVDVCPMDCITFTDNGDEAELRQRLNAPALNPEQALLVSPAVKTGRVMVKDEDVC
                     LHCGLCAERCPTGAWDMQKFLLFTAKAGGASDSLGVHPAQEVRA"
     gene            24255..25646
                     /locus_tag="PKN51_03540"
     CDS             24255..25646
                     /locus_tag="PKN51_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNT84204.1"
                     /translation="MLDEGGQVRPHYRAFAQWLAAQPPQAMAARRLEADLNFRRIGIT
                     FSMAGDADGTERLIPFDLIPRVIPAAEWRWLDRGLKQRVRALNMFIHDIYHDHDIVRA
                     GIVPPEQVFLNAQYRPEMQDVTVAENIYCHIAGIDLVRANAGEFYVLEDNLRVPSGVS
                     YMLENRKMSMRLMPEMFSRERVAPVAHYPDMLLDNLREVAPRNIDDPTVVVLTPGMYN
                     SAYFEHAFLAQQMGVELVEGKDLFVDQNTVYMRTTRGPQRVDVIYRRVDDDFLDPLTF
                     RGDSALGVPGLLSACRARRITLANAIGTGLADDKSTYLFVPDMIRFYLGEEPILSNVP
                     TWQCSRPKELSHVLAHLPELVVKEVHGAGGYGMLVGPTASRAEIEAFRARIQANPAHY
                     IAQPTLALSTTPTYVESGVAPRHVDLRPYVLCGKEIRTVPGGLCRVALKEGSLVVNSS
                     QGGGTKDTWVLEE"
     gene            25673..26653
                     /locus_tag="PKN51_03545"
     CDS             25673..26653
                     /locus_tag="PKN51_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNT84205.1"
                     /translation="MLSRTADHLFWMCRYIERAENTARMLHVNTQLALLPRDAAVHRQ
                     SWQAMLRLFELEDDFAARYNDPEPQAVIHFMVRDADNPSSIYACLRACRENARAVRGS
                     LTTELWETINLTWLTLLRDLDEGVLERNPEQFFEWIKRRAHLVRGVTENTMLVDEALH
                     FMRLGSQLERADNMARMLDVRFHEHEALAGGGAPAAQGKALDADAGEFYRWSAILSCV
                     SALEVYRKVYRDVVTPSRVAELLVLNTTLPSSLVALVRGVRESLARVANDRSAETERQ
                     AGLLLAELEYGRIEDILRGPGLHRFLTNFLARINHLGTRIGSDFLLPLSA"
     gene            26711..27502
                     /locus_tag="PKN51_03550"
     CDS             26711..27502
                     /locus_tag="PKN51_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNT84206.1"
                     /translation="MRHLIRHTTRYHYTDPVSYSIQLLRLTPRRDEHQRVLRWNIEAP
                     GLLEEQVDAYGNVTHTLTLTQPHETIELKVTGQIDVAPLDDGALPGEDSRLPWQAYCV
                     PTQLTACDDSIRAFAQATLPGGLRGPDDALALAEAIERRVAYEPGITDVRTAASQVLA
                     LGHGVCQDHAHLFLACARALGRPARYVSGYLHTDATHMASHAWADLWWPEQGWVSIDV
                     TNRQYASERHCRLAVARDYESASPVRGVRTGGGAETMLVDVLVQQ"
     gene            27579..28451
                     /locus_tag="PKN51_03555"
     CDS             27579..28451
                     /locus_tag="PKN51_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNT84207.1"
                     /translation="MTYCVAACLDAGMVFLADSRTNAGVDQISTCRKLRVFERPGERL
                     LALMTSGNLAISQAIVALLAQRGPDDPQSIWNAATMQEAAQCVGDAVRAVHARDGQAL
                     KEQGIDFNVSLILGGQIGRAPCQLFQVYAAGNFIAASPEGPYLQIGEAKYGKPIVDRV
                     LQAHTPLDEAAKCMLISMDSTLRSNISVGLPLDLLVYERDALRVTRFACLDEDNQYLD
                     MIRRTWGERLRQVFAEIPNPQWTTPEAQAGDAALPLSRRHQPVRIAAGPEPQGQPPAV
                     HQVLAQGAGRRQVQ"
     gene            complement(28535..28996)
                     /locus_tag="PKN51_03560"
     CDS             complement(28535..28996)
                     /locus_tag="PKN51_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT84208.1"
                     /translation="MTRPFKVLGIQQVAIGGTDKSRMKRLWVDMLGLTQTGQFQSERE
                     NVDEDILAMGSGAHKVEVDIMQPLDIDKKPAVHTTPLNHIGLWIDDLPKAVEWLTAQG
                     VRFAPGGIRKGAAGYDITFMHPKASDEFPIAGEGVLIELVQAPPEVIAALG"
     gene            complement(29061..29684)
                     /gene="pncA"
                     /locus_tag="PKN51_03565"
     CDS             complement(29061..29684)
                     /gene="pncA"
                     /locus_tag="PKN51_03565"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HNT84209.1"
                     /translation="MNPQTALIVVDAQYGFMPGGGLPVADGDAVVPVINRIAPRFANL
                     VLTQDWHPADHVSFAANHPGRQPFETIALPYGVQVLWHTHCVQGTRDAALHHDLRVDH
                     AQLIIRKGFRRDIDSYSAFVEADRRTSTGLAAYLHARGIQRLFLCGLATDYCVAWSAL
                     DARAAGFEVTVIEDACRAIDLDGSLARAWADMAAAGVQRVQARHLNE"
     gene            complement(29793..30596)
                     /locus_tag="PKN51_03570"
     CDS             complement(29793..30596)
                     /locus_tag="PKN51_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNT84210.1"
                     /translation="MATAWPDVQSVVALQRGGVRFEFYRAGVSADSLQDVQSVTKSVL
                     ALLFGCALADGRVRGVDELVAQRLPRLLRLGGDARLRRLRFAHLLSMTAGWPGELTAR
                     RDRDDDLRWLALRPFVAEPGARFAYDNGAANLLALALANAVGQPLAEYARERLLAPLG
                     ITRLAWRRGRRGRRWGRWVCRSVCATWRGWASWCWPKAAGAGGRCCRPTTCARPPRGA
                     TPAARRCSRPMATCGGCRPSGRRAGTRAAPSWPAAMAASGCTSIRSATR"
     gene            complement(30757..31071)
                     /locus_tag="PKN51_03575"
     CDS             complement(30757..31071)
                     /locus_tag="PKN51_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNT84211.1"
                     /translation="MHLLKLPIHATPGARRTEAAGAHGQALRVRLAAPPVDGKANAAL
                     IAWAAAALGVPRSQVELLHGASGRQKVLGVRFDDAAALAAAQAQIAQWLAGGADGQSG
                     GY"
     gene            complement(31087..>32283)
                     /locus_tag="PKN51_03580"
     CDS             complement(31087..>32283)
                     /locus_tag="PKN51_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl-CoA carboxylase subunit alpha"
                     /protein_id="HNT84212.1"
                     /translation="MNTRLQVEHPVTECITGLDLVELMIRVAAGEKLPITQQDVKRDG
                     WAIECRINAEDPFRNFLPSTGRLVRFMPPAETMWQADTEHRYGVRVDTGVVDGGEIPM
                     YYDSMIAKLIVHGKDRQDAIARMREALNGFVIKGVQSNIPFQAALLAHPSFVAGDFNT
                     GFIAEHYAHGFSAADVPHADPDFLLALAARLHMYYRNRAQGLQGQFWEGFKSLPSREF
                     TVCALGKEGQHQYVDVSLGEVQRDEPTRVVVRGAAGERVYELKLDRYLGQSRITGECN
                     GQRFTAQMERGHTRNPLVTRVIHNGTQLDAIVMHPRTAELHRLMPYKAPPDMSKFVLS
                     PMPGLLVQVAVQPGQKVQAGERVAVIEAMKMENVLLASADGVVKEVKAQQGESLAVDQ
                     AIVEFE"
BASE COUNT         4916 a        11471 c        11069 g         4827 t
ORIGIN      
        1 atcagcacct ggaacgtcaa ctccctcacc gcccgcctgc cgcacgtgct ggactggctg
       61 gcggccaatc cggtcgacgt gctggcgctg caggagctga agctgacgga cgacaagttt
      121 ccgcatcagg cgctgggcga agccggctac gccgccgtgg ccttcgggca gaagacctac
      181 aacggcgtcg ccctgctgac gcgcgcgccg ctggcggcgc aggcggtggt gcgcaacatc
      241 cccggttttg ccgacgaatc ggcgcgcgtg atcgccgcca cgctggaggc gcccggcggg
      301 ccgctgcggg tggtcaacgg ctatttcgtc aacggccagg cgccgggaac ggacaagttc
      361 gactacaaga tgagctggct gcgcgcgctg cgcgactggc tgcgcgagga gctggcggcg
      421 cacccgcgcc tggtgctgct gggcgacttc aacatcgcgc cggaagaccg cgacagctac
      481 gacccggtgg gcctggccga aaccattcac cacaccagcg aggagcgcca ccacttccag
      541 gccctgctgg ggctgggcct ggcggacgcc ttccggctgt tcgagcagcc ggacaagagc
      601 ttcagctggt gggactaccg catgctgggc taccagaaaa accgcggctt gcgcatcgac
      661 cacatcctga tcagtcagcc gctggtggcg caggcgacgg cgtgccatat cgaccgcgtg
      721 ccgcgcaaat ggcccaagcc cagcgaccat gcgccggtga cgctcgaact ggcctgaagc
      781 gcacggccca tgaatgccac agcccaatcg ggctgtagcg cgctaccaat ctccgccaac
      841 agctattatt acaatagcaa acgagcggcg aagcgattca gcctaggagg ctgtcgggct
      901 tggagcgccg aaagcgagaa tcggccccag cgagaccagt ttttgccgga tttgcagccc
      961 aatagtcgag ctattgggca aaaagccggt gaaaaatggg ccgctgcggt cgatttgcag
     1021 ccggcgattt ccaagtccga cagcctccta ggttcaggcc acgcggcatc ggccctgcga
     1081 tacggggtac cctgcatggg gacgggatgc cgcacgccaa gcgctgacac ggccgcagcc
     1141 gtacccggca ttggctgcgc agaccgctcc gggccgggag gcgccgcgcg ccagggggtt
     1201 gttcagcgtc gcctcaaggt ccaaggcggc ccagctcgcg cttgatgcct tcgatgtcgc
     1261 tctggttgcg ctcgatcgcg gccttcagtt cggccacgcg gtcgaggtac ttctggtggt
     1321 tgcgcgcttc gccgccgatc ttgtcgggct caccaccctt gtactccttc tccagctcgg
     1381 cttgcttggc ctcggcgcgg cgcaactcct gctgcaggat ggccttggcg tcggcgtcgc
     1441 gcttgcgctg ctcggcgttg tcgacgcgcg gcgccgaggc gggtgaagcg ccgcccgagc
     1501 gcgccgccgg ggccgtcccg gcgggggctg tggccgccgg cgccgccgcg ggtgcggtgc
     1561 cgcgcacgat ggtgacgttg ccgccttcca ccagcttgca gtcggagcgg cccttgggtt
     1621 ggttggtgta ctcgttgccg cagcggtaga tgcggtcctg cgccgccgca ggccacgccg
     1681 cgacggccag cgcggcggcg agcaacggca cccaggggat cggcagcaga ggtttcatgt
     1741 cggtcggtac aacggccccg gcaacgcgcc gggatcagcc ttcaacgatc aagtatcggg
     1801 caggtggacc gctttggggc aggcttgttc ccgaaaatcg ccactgaacc ggcccaggcc
     1861 gccgttgggc ggcccatttc gtgtcattgt ggcgacaagc gcggtcagag cgagaagtac
     1921 aggtcgtact cggccgggtt gacctgcacg cggatgcggt tgacctcggt catcttcagc
     1981 tcgatgtagg cgtcgagcat ggcgtcggtg aacacgccgc cgcgcgtcag gaaggcgcgg
     2041 tcgcgatcca gctcttccag cgcctggtcc aggctgtggc acaccgtggg caccagcttg
     2101 tcttcttcgg gcggcaggtg gtagaggtcc ttggtggcgg cttcacccgg gtggatcttg
     2161 ttctcgatgc cgtccaggcc ggccatcagc aacgccgcaa agcacagata ggggttggcc
     2221 atggggtcgg gaaagcgcgc ttcgatgcgg cgcgccttgt cgctggcggc gtgcgggatg
     2281 cggatcgagg ccgagcggtt gcgcgccgag taggccagtt tgaccggcgc ctcgaagtgc
     2341 ggcaccaggc gcttgtagct gttggtggtg gggttggtga tggcgttgag tgcccgggcg
     2401 tggcggatga tgccgccgat gtagaacagc gccgtctcgg acaggccagc gtagcccttg
     2461 ccggcaaaca ggttcttgcc gcccttccag atcgactggt gcacgtgcat gccgctgccg
     2521 ttgtcgccgt ggtagggctt gggcatgaag gtggccgtct tgccgtagcg gtcggccacg
     2581 ttccagacca cgtatttcag gcgctgcgtc cagtcggcgc gctcgaccag ggtggaaaag
     2641 cgcgtgccga tttccatctg gccggcgccg gcggtttcgt ggtggaacac ctccaccggc
     2701 acccccaggg cgtccaggat cagcgccatt tcggcgcgca tgtcgtgggt gctgtcgacc
     2761 ggcggcaccg ggaaatagcc gcctttggtg cgcgggcggt ggccgcggtt gccgccttcg
     2821 agctcggcgc cggtgttcca gggcgcttcg tattcctcga tgcggtagaa gctgtggccc
     2881 ggctcggtgc cccagcgcac gctgtcgaag acgaagaatt ccggctcggg gccgaagtag
     2941 gcggtgtcgc cgatgccgct ggccttcagg taggcctcgg cgcggcgcgc gatggagcgc
     3001 gggtcgcgtt cgtaggcctt gccgtcgccg ggctccagca cgtcgcaggt cagcaccagg
     3061 gcgggctcct cgaagaacgg gtcgacgtag gccgtgttcg ggtcgggcat cagcagcatg
     3121 tcggacgcct cgatgccctt ccagccggcg accgaggagc tgtcgaacgg gtggccgtcg
     3181 ctgaacttgt cttcgtcgaa ggcggtcacg ggcacggtga cgtgctgctc cttgccacgg
     3241 gtgtcggtga agcgcaggtc gacgaacttg cattcggctt cctgcagcag tttcatgacg
     3301 tcggcgaccg atcgggtcat cggggtactc ctgaggggat ggggatgggg atgggtgaat
     3361 ggcagagcgc cggccccagg ccggcgtgcc gcccgggcgg gcggcgtccg gcaggtggcc
     3421 tgcgcgaaag ggtggatttt gcctgttgcc gggcggcaag ttcgccgcgc ggcctgcgcg
     3481 cctcagccac gcaccagttc tggcccgtag gacagcgcaa acggctgcag ccaggcgcgc
     3541 aggtcggcgt aggcggcgtc ggcctgcgcc ggcgcgccct tgacgcccag ctcgatgtgg
     3601 cgcccgtgct cggggtgatc gacgctgggc aggctgaaca ccctgacgcc ggggtgcgcc
     3661 gcctccaccc gctccatcac cggcgtcagc ggcgcttcca tggcgccgta caacaccacc
     3721 gagcgttcgc gccaggcgcc gcgggtgaag cagtgcgacc attcggtgtc cagcgcccag
     3781 gccatcatgg gccaggccat gaccgggaaa cccggtacga aatagatagc acctcccgca
     3841 ggccccgcaa gcgcaaaacc cggaatctgg ttgtaagggt tgggcacgat gcgggcccct
     3901 gcggggaaca cgcccatgtt cagccgctgg cggttgtcgg cgcgctcggg ctcccagggc
     3961 tggccctgct ggcgcgccat gtcctggccg cgctgctcga tcaggcgccg ggcctcgggg
     4021 tgcagggcca gcggcacccc cagggcggcg gcggcgcact ggcgcgtgtg gtcgtcgggc
     4081 gtcgcgccga tgccgccggt gcacaccacc acgtcgcccg tggccagggc gcgccgcagc
     4141 gtggcggtaa tgcgcgccgg ttcgtcgccc acgtattcgg cccaggccag gcgcaggccg
     4201 cgctcggtca gcagctcgac gaacttgggc aggtgcttgt cggcacgctt gccggagagg
     4261 atttcgtcac cgacgacgat gaggccgaat tcgggcatgg ggtttcaggc agaaccgttg
     4321 agatcgcgcc acggcaggtc ggtcacgcgg tcgggtgctt gcgccgccgg cagcgccgcc
     4381 gccggcgcgg tgcccggcgg gcgctggtcg cgcaggcgct gcagcgcgtc gagcgcatag
     4441 tgcgcgaacc acagcgagga aaacacgaag acgaaggtgt agatccagat cgccagcggc
     4501 accagcagca cgaacagcac ggcaaacagc gccatcgagg cccagacgat gcccggggcg
     4561 gcgcccaggt agccacagat cacgccgatc agcagcagcg gccagcggtg ctggcgcaag
     4621 atggtgcggc gttcttcggc gctggcgtgg tcgaccaggg cgtcgaagct catcacccgg
     4681 taggtcagcc agccccagat gagcggcggc agcaccagca ccagcggcgg aatcagccac
     4741 agcggcagcg tcagcaccag cgccagcacg gccagcagca gcgagccggt cgaccacgcc
     4801 agggaagcgg ccagcgagcc cccgcccagc cgcgccaggt tggcaaagcg ccgttcggcc
     4861 accagccgcg tgagcgccgg tgtcagcagc agcgacacca ccaccagcga cgccagcacg
     4921 atcaccggcg tggccagcag gaccacgaac agcggcgcca gcagcagcgc caggccgccc
     4981 ggccgctgga tgttcagcca gccctcggcc cagcccagcg gcgaccagcc ctgcagcgcc
     5041 gcctgcatcc aggccaccgc cgcctgccag cccagccacg ccagcagcag cgccaccacg
     5101 ctcatcacca gcagcggcag cagcgtcatg ccgatgacgc gcggcagaaa cacgtggccc
     5161 aaggcacgca ggaaggcgtc gaacacgcgg ggcagcatgg cgtcaggata gcgcggcgcg
     5221 ccgcccgctc aggcgcgccc ggccaggcgc agcaggcccc gccactgctg ggcccagaag
     5281 ccgcgcccgt agtcgcgccc ctgctcgacc tgatcgcgga tgccggtggg ctcgcagcgg
     5341 ccggaaaagt cggccgagcc cagcagcgcg tcccagcacg gcagcagcac gccgaagttg
     5401 acgccccgga catgggcccc cacgctcccc gctgcgcgtg gggcgctgcc ccccaggggg
     5461 gcgctcgcgc cttgggacgg cccggcggcg cccggttcgc gcgcggctct ggcctcgtgg
     5521 ccgatgccga tggcgtggtg gcggcggtga aagcgcgggc tgaccagcag gcgctcgccc
     5581 acgcggccga acgaccagcg caggttggcg tgctgcaggc tttcgagcag ctgcgtgcac
     5641 gccacgagca gcacgaactg cgacggcgcc acgccgatca ggatggcgac cacgaccatg
     5701 atggcgtcgg tcagcacgct gtccagcagg tggttgcggt cgtcgctcca catcgtcatc
     5761 tggcgctgcg aatggtgcac ggcgtgcagc ttccaccacc agtcccactg gtgctgggcg
     5821 cggtgcaccc agtagccgat gaagtcgaac accagcaggt acagcacgaa gctgaccagc
     5881 ggccggtcgg tcacgcccgg ccaaagcgcg tccaggtgca gcgtcggcag gccgcgcgcg
     5941 cgcagcacgc cgatggcgtc ggtcagccag ccctcgacgc tgaagaacat cgccagcttg
     6001 aacagcccca gccggtgcag cagggtgtag agcacgtcga cgcggatggc cgcgcgatcg
     6061 cgcaccggct cggccggcca caggcgctgc agcgggccga tcacggccag cagcacgccg
     6121 atctgcagca cgccgatcag cagccagccg gcggccacga agccgtcttc cagcaggttg
     6181 cccaggccca gggcgaaggc gagcggctcg atcagggtct cgaacagccc ctgctgggcg
     6241 ctggcgaaca ggtcggcggg cgtcatgctg cgggtaccgc ctatgcgcgc tgctgcgcga
     6301 tccagggcgc gtactgcggg tgctcgcgca gcgtgcggaa cacgaagccg cgcgccttca
     6361 gcccctcgat cagcggctcc agcaccgccg gcgcccacgg gtcctggcgc gaccagatgc
     6421 ccaggtgcgc cagcaggatg tcgcccggct ggatggtctt gagcgcacgc tcgagcagca
     6481 gtcggttggg gtatttgtcg ctcggcagtt catcgcccag aaagccggcc ggcgaccagc
     6541 ccacgtgctc gtagccgcag gcgcgtgcgg cggccagcag gcgcggcgag atcttgccgc
     6601 ccggcgcgcg aaacagcggc aggggttttc tgccgctgta gtaggccagg cgctcgctgg
     6661 cctgctcgat gttctggcag tactgctcgg cgctccaggt gaattcgcgc ccggccaggg
     6721 ggccggccga ggggcgcacg cgaaagcgcg gctcgcgccc gggcaggtcg gcgcgccagt
     6781 acacgtggtc ccaggtgtgc gaggccagct cgtgcccttc ggcggcgcgc gccttccacc
     6841 acggacccca atggcggctc aggctgccgt cgccctcctg cgtacgttcg ttggcggcaa
     6901 agaacgtcac gcgcacctgc tgacggcgca gcacctcggc caccagcgcc gccacgccca
     6961 tgtggccggt gtcgaagctc aggtacagcg gggcgctgcc agatgcaggg ccattttggc
     7021 ctctggcgct tgtccagagc gcgccggcag ctatcgaaaa aagagcattg cggcgttgca
     7081 cccgaggcct ccgctcacac ccttggggca tggtccaggg tccacacgcc gtgcggcgac
     7141 ttgcccacct tgacctggtg caccagcttg cgctgcgcca cgtcgatcag gctcagcttg
     7201 ccggcccagc gcgagccgac catgatcagc gaaccgtcgg ccgacacgtc catgcagtcc
     7261 gggccggccg gcgccgggaa ttcggccacc accgccagcc gcgtggtgtc gatctggctg
     7321 atgctgcccg ccacgcggtt gctcaccagc acgtggcggc ggtcgcccag cgcgcgaaac
     7381 gcgtgcgcgc ccttgccggt ggccaccagg ccgacgcgcc gcggcgcctg gccggcggcc
     7441 agctcgaaca cctcgacccc gctgccgccg gtcaggccca gcagcaggtg ccgctcgtcg
     7501 ggcgtgacga acacgtcggc cggcagcggc ccggtgagca cgcggtgcat cagttgctgc
     7561 gacgccaggt cgatcgccac cagctcgtgg ctgtcctgca tggtggccca cacggtggtg
     7621 ctgcgcgcgt cgatccacag gtggctgggc gtgcgcccgg tcgggatgcg ccgcgccagg
     7681 tgcggctgct gcccgtccca gtggtagagg tcgatgtggt tgagccggtt ggccgccgtc
     7741 acgaaccagc gcatgtcggg cgaaaagcgc aggtggtagg ggtcgaggat gtcgcgcagc
     7801 gtgcgctgca cgatggccgt gcgcgggtcg atgaaggtga gcgaatcgct gccggcgttg
     7861 gcgacgatga ccgaccgctc gtccggcgtc atgtacaggt ggtgcggctc cttgccggtc
     7921 gggatgcgca ccagctcgac ccagcgccgg ccgtcgatca cgctcaccgt gccgtcgagc
     7981 gagttgagca ccagcaccgg cgctgcggtc ggcgccgcca gcgcaccggg cgccagcgcc
     8041 gccgcgccca ggcccactcc ggcggccagc agccggcgac gcgtcgggga aggaatgtcg
     8101 atcgatgaca agacggacct gtggaaaaaa agcgtgggcg gtggcgcgcg cgagtgtaaa
     8161 cccgcacccg ccccattcac gatggattcg accaatggtg tctggaatgc cgtgcgggct
     8221 gaactcgtcc aagcccttcg acaggctcag ggtgaacggt tgaaacttca agcttccgaa
     8281 gcaataacat gccagccatg cgatcggtgg acatcgacgg cgtcagcctg gagcttcagc
     8341 acatcgcgcc gccggccggt gcggcgccgg cccgggcacc gctggtgttt ctgcacgagg
     8401 gcctgggctc ggtggcgctg tggcgcgact ggccggcgcg cctgtgcgcc gccaccggtc
     8461 gcgccggctg ggtgtattcg cgtcgcggct atgggcgctc cagcccggtg cccgacgtgc
     8521 gcggcgccgg ccgcctgccg cccgactacc tgcaccgtga ggcgctcgac gtgctgccgg
     8581 ccctgctgcg agcgctgggc ctggcgcgcc cggtgctggt gggccattcc gacggcgcca
     8641 gcatcgccct gatccacgcc gcccaccacc cggtcacggc ctgcgtggcg atggcgccgc
     8701 acgtgatggt ggaagcgctg tgcctgcgcg ccatcgagca gacgcgccag cacttcgaga
     8761 ccggcgccct gcgcatccgc ctgcaacgct ttcatgacga tgtggatggc gccttctggc
     8821 agtggaacga cgcctggctg aacccgcaat tccacggctt cgacatccgc gccgacatcc
     8881 gcagcatcga cgcgccactg ctggcgatcc agggcgtgga cgacggctac gccagcatgg
     8941 cccagctcga cgacatcgcc acggcgctgc cgcacacgcg tctgctgaag ctgccggcct
     9001 gcgggcacgc gccgcaccag gaccagccgg cggccgtcca gcaggccatc accgaccttc
     9061 tggccgatct gccctgatgc gcggcggcgc gtccaagccc atcgatcacg gctcgttcgc
     9121 tggcaggcgt cgccgcgtca ggggcgcagc aagtccagct cggaaggctc cagccagcgc
     9181 cactgccccg gcgccagctc ggccggcaat gacaggccgc cgatgcgcga gcggtgcagc
     9241 gtttcgaccc ggttgccgac ggcggccagc atgcgcttga cctggtggta cttgccctcg
     9301 gtgagcgtca gccgcagttg acgttcgccg ctcgcctcgg ccgccgccgc caccaccggg
     9361 cgcgggtcat cgtccagcac cacgccgctg cgcaggcgct ccagctggcg ttcatccagg
     9421 gggtgccggg tgcccacctc gtagaccttg ggcacatgcc gacgtggcgc attcatgcgg
     9481 tggatgaagg cgccgtcgtc gctcagcagc aacaggccgg tggtgtcctg gtccagcctt
     9541 ccgaccgcct gcaccccggc caccttgccc gccgtgggcc gctggcgcag cggcgccggc
     9601 agcagtgtgt agacgctggg ccagcgcccg gggcggtgcg agcactcgaa gcctgccggc
     9661 ttgtgcagca tcaggtaggc ccgcgggtgg taggtccagg cctggccctg aacctcgtag
     9721 cgcaacgggc cggccgacag gtccacctcg gccgacggat cggtgcaccg ccgctcgccc
     9781 aggcgcacca gcccctgctc gaccaggccg gcgcagaccc ggcgcgtgcc gaagccctgg
     9841 ctgaacagca gttcgctcag gcgcatgcat gacttccatc aaactgtgat gaatcgagag
     9901 ttggcatgac ttattgctca gaaatccggg acccggactg aaattagaca aaaagtacct
     9961 tcttattcgg gcggcgcgtg ggtaacatca tgcaacgaaa tgccgcgcca tccgcgcgcc
    10021 acagggggaa caagattttg agcgccatct ccggttcgac caccttctgg gccatcgtcc
    10081 tggccatagt gggtctgttc accggctacc tcggcctgag cagcttcatg ccgcgcggcc
    10141 gcgatggaca ggcgggcaag ccccgcagca agctgcgcat gccgcagcag tggccgctgg
    10201 tcccaagacc gctggccaac agccgcgagc gcctggtgtg gcactggttg cgcaaggtct
    10261 tccccgggca tcacatcatg gtcaagctgc cggtcacgcg cttcaccatg ccgcgccagc
    10321 cgggcgaggc cagcgaatgg ttcgagctgc tcagccgcgc ctattgcagc ttcaccctgt
    10381 gcgacagcaa gggccatgtt atcggctgcg tggacgtgct ggggccccac ggactgtcgc
    10441 gcagcaaccg ccagctcaag cagaccctgc tggcccaatg cggcatcgcc tactgggtga
    10501 tctcgcccga tgcgctgccc gagccgtcgg cgctgcgggc cgacttcctg ggcgtcaacc
    10561 cggccgacct cgaagccatg ccggcaagca gcttcgccga gctggaagcc gcacgccacc
    10621 acctgcacca gacgctggat cgcaaccgca gccagcgcgc accgggcccg gcgcccgacc
    10681 cgaccccggc gcacgagggc gacgcgccgt cgggctgggc gcaggccgac tcgttcctgg
    10741 gcagcctgga cagccgccgc acgcccctcg atcgtcattg acccccacag gtccacacgg
    10801 tcgcggcggt cgaaagacag gcatccgtgc cgggtgcccg aggcaccaag cacacggcca
    10861 gcctgggcaa cgaccgacgg ctgccggggc ggtccaggtg gctcaggtgg ccagccggat
    10921 gaaatgctcg cggtagtgct ccagctcgtc gatcgactcg tgcacgtcgg ccagcgcggt
    10981 gtgcgactgc tgctttctga acgcctccac cagttccggc cgccagcggc gcgccagctc
    11041 cttgagggtg ctgacatcga cgttgcggta atggaagaac gcctccaggc gcggcatgta
    11101 gcgcaccaga aagcgccggt cctgcccgat cgagttgccg cacagcggcg acacgccctt
    11161 ggacacgtag cgcgacagaa actcgatcag ttgcgcctcg gcctgcgcct cggtcagcgt
    11221 cgaggccttc accttgtcga tcagcccgct gcggccatgc gtgcccttgt tccaggcgtc
    11281 catggcgttg agcacgctgt cgggctgatg gatcaccagc accgggcctt cgatgcgcgg
    11341 ctcgagctgc ggcccggtga cgatgacggc gatctcgatc aggcgttcct tttcggggtc
    11401 caggccggtc atttcgcagt ccagccagac caggttcaga tccgatctgg ccagggcggg
    11461 ggcggtgtct tgaggggtgc aaacggtgtc ggtcatggcg ccgattgtcc accgccggcg
    11521 ccgggccgcc tgcaggccgg tccttgccgt cggcggctac accctaaact tcgctgtcct
    11581 gcgccgtcct ccccaccccg tccgtgttga acccaacgcc ctcgcccgcc ctcctcacca
    11641 ccctggtgtt cagtcttgcg ctgctgctgt acgtggcggt caagttctgg ctggcctcgc
    11701 gccagatgcg cgccgtggcg gcccaccggg ccgccgtgcc ggccgagttt gccggcacca
    11761 tcgggctggc ggcgcaccag cgcgcggccg actacaccat cgccaagctg cgcttcggcc
    11821 tgctggagct gctgctggcc gccgccgtgc tgctgggctg gacgctgctg ggcgggctgg
    11881 acgcgctcaa cggcgccctg ctcggcgccg tggggccgcg cccgctggcg cagccgctgg
    11941 cgctgctggt ggtgttcgcc ctcatcaacg gcgccatcga tatcccgggc gcgctctacc
    12001 acaccttcgt gatcgagcag cgctttggtt tcaacaccat gagctggcgc ctgtggttga
    12061 tcgacctgct caagaccgcc ctggtcagca cggtcatcgg cgtgccgctg gccgccctgg
    12121 tgctgtggct gatgcaggcc acggggacgc tgtggtggct gtggacctgg ggcgcctgga
    12181 tgggcttcag cctgctgatg ctggtgctct acccgaccgt catcgcgccg ctgttcaacc
    12241 gtttcgagcc gctgcgcgac gacgcgctga ccgagcgcgt gacccggctc atggcgcgct
    12301 gcggctttgc cgcccagggc ctgttcgtga tggacggcag ccgacgcagc gcgcacgcca
    12361 acgcctattt caccggcttc ggcgccgcca agcgggtggt gttcttcgac accctgctgc
    12421 agcagctcag cccgcccgag gtggaggcgg tgctggcgca cgagctgggg cacttccggc
    12481 accgccacat cgtcaagcgc atcgccgtgc tgtttgcggt cagccttgcg gggctggcgc
    12541 tgctgggctg gctggccgag cagccgtggt tctacagcgg cctgggtgtg acgcccaacc
    12601 tgccgccgca gatgggcggc gcggtgccca accacgcgct ggcgctgctg ctgttcatgc
    12661 tggtggtgcc ggtgttcagc ttcttcctgt cgccgctgat ggcggcgctg tcgcgccgcc
    12721 acgagttcga ggccgacgcc tacgccgtcc gccaggccag cgcgcaggac ctgggcaatg
    12781 ccctggtcaa gctctacaag gacaacgcca gcacgctgac gcccgacccg ctgtacgtgc
    12841 gcttttacta ctcgcacccg ccggccagcg aacgcatcgc ccgcctgggt gcccgggtgc
    12901 tgccggtgtg agcgctgcca gccgccgcgt gcgcctcatg ccaaatcggg cgctggcgct
    12961 tgcccatcaa gcgccagcag ctattgtttt catagtttcc gacgcccgtc gcggtgccgg
    13021 cccgaaccca tgagccgcgc ccgcccaccg tcggccccgc gcaccgccgc gccagcgctg
    13081 cacgacggcc tggtggtggc cagccacggc cgccactgcg tggtcgagct gccggcgggc
    13141 gagcgcgtga tctgccaccc gcgtggcaag aagctgggcg tggtggtggg cgatcgggtg
    13201 cgctggcggc cctcgcacga cgagggcacg gtcgagcaga tcgcgccacg gcgcaacctg
    13261 ctttaccggc aggacgccgt gcgcaccaag agctttgccg ccaatctgga ccaggtgctg
    13321 atcctgctgg cggccgagcc ggaatattcc gaacaccagc tggcgcgcgc cctgatcgcc
    13381 tgcgaggcgg ccgacatcga accgctgatc gccctcaaca aggccgacct ggcggcgccg
    13441 ttcgagcgcg cctggcagcg cctggcgccc taccgcgcca tgggctatgc ggtgctgccg
    13501 ctggccgccc ggccgcaggg cggcgcggcg cccgccgacg acggcctggc cgcgctggcc
    13561 cagcgcctgc aagcccgcac gacgctggtg ctgggccctt cgggcgcggg caagagcacc
    13621 ctgatcaacc gcctggtgcc gggggccgcg gcgcagacgg gcgagatctc gcaggcgctg
    13681 ggcagcggcc gccacaccac caccaccacc acctggtact ggctggacgc ggcgcgccgc
    13741 agcgcgctga tcgactcgcc gggcttccag gaattcggcc tgcaccacat cgacccgatg
    13801 cagctggcgg cctacatgcc cgacctgcgc ccgcatgtgg cggcgtgccg gttctacaac
    13861 tgcacccatt tgcacgaacc gggctgcggc gtgcgggcgg cccagggttt tgacactgaa
    13921 tcgggctctg gcgcccaggg gatcagcgcc agccgctaca aaatctacag cgacctgttt
    13981 gccgagctgc gctccggcgc ctgacgccgc ggcgcgccgg ggctcagcgc ggcggcgcca
    14041 ccaggtccat gcggcggcgc aggcgcgcgg cgtcctcggg gcgtccggcc tgttgcgcca
    14101 ggcgctgctg cacgcccagc caggccagca gcgggcggcg ccagccctgc tgcgacgcgg
    14161 tgtcgaccgc ctgcgccacc acggccgggc tggctcggcc ctgacgcagc agcacgccgg
    14221 cggcgatcag gcgcgccagc gggtcgtcga tggcggccac ggcatcgccc ccggcagcga
    14281 tggcgcggtg cggcggcggc agcagcgacg cctgggccgc agcagcctgg ccggccagat
    14341 agtcggcgta agcgcgctcg ggctcgcccg cgtccgcgcg cagtggctcg aacccggcgc
    14401 agggcgccag ctccaggctg gccacacgcg tcgcgcagcg cgtcagctcg acgcgcgcca
    14461 ccagctcgga gcggccggtg cgcgccacct cggcgcgcgc cagctcgaat tcgcgcgcct
    14521 cgacgcgcgc atcgccgcgc agggcggctt cggcaaagcg ctccacatgg cccaccgcct
    14581 ggatgttcca gtcaggcact ttctggccgc cagcgcaggc ccacaaagcg ctggaagcta
    14641 tcacgaagat agcactgtgc agaccacgcc aggcgttcat ggcaaacgga cctcctgctg
    14701 acggggcgcg aacggccatt tgcggttcag ttccgtgatc agcgcgtcca ccttgcgcag
    14761 gctggactcg acgtcggcgc gcagcgcgcc caggtcggtg ctggcctcgc gcgcattggc
    14821 ggccacgccc tgcacctcgg tcagcacggc atcgaccttg accatgctct ggcgcagctc
    14881 ctgcagcagc gcgcccagct gccgcaccgc cacctgcgcg tcggtcatca ggccgtcctg
    14941 gcccagcacc tggcggtcgg cgcgctgcac cacgctgtcg acgcgctgcg ccaccgcgtc
    15001 gagccggcgg accagctgcg tggtctgtcc cagcagctcg ctcacgcgcc gtgcatcggc
    15061 ttcgttgccg gtgacggccg ccatcagccc accctggctg ctgttcaggc gctgggtgaa
    15121 gcgctgcacc tcgcccagcg tggcgtagag cgccgactcc tcgccggtca ggcgattgag
    15181 gttgagcagc acgtcgcgcg cgtcggccac catcttgggg atttcggccg acacgtcgcc
    15241 gcgcagcacg atgcgctcgg cccccggtgg cagcggcggg tcgtccaaaa tgccgctgaa
    15301 ggcgcgcagg cgcgcggccc cgaccaggcc cttttccagc gtgaagacgc tggaggtgcg
    15361 cagccagcgc gccgatttga ccggtacatc cacatggatg cgcaccgtgc cctgatcggc
    15421 cagctcgatg cggctgacgc ggccgatcgg aaagccggaa aagctcatgt ccatgcccac
    15481 caccacgcct tcggagtcgt cggcgctcag gtacagcgtc tgcgtctcct cgaaggcgcc
    15541 gcgcacccac atcaggtaca gcacggcggc acccaccagc agcgccatga acagcaacag
    15601 cagcaccgcc cgccgctcca gactgcgcag cggcacgcgc gccacctgcg ccacggcgtc
    15661 gggcgcgtcg gccggggcgg cggagggttc agtggaggtc atggcggctc aatagtaatt
    15721 gccgatcagc gaggcgactt cgatcacaag catcagcacc gacatctgca ccagcccctg
    15781 cagctcgacg ctggtgcgcg agccgcggtg cgggatgtcg cgcagcgcat tggccaccgg
    15841 cagcagcgcc accgccagcg cgaacagcag cgtcttcatg ctgaagatca tcgacaccac
    15901 cgggttgaag atagtgccca ccgcgtggtt gtacgacggc agccccgcca gcgtgaagcc
    15961 gtgcaccgcc acataggcca gcagcagcga gatcaggcag ctcagcagcg ccagcagcag
    16021 cacggcaaac atgccggcca gcacgcgcgg cagcacctcg tggctgagcg gctcgcagcc
    16081 gcgtgcgcgc agcgcctcca gggcgccgcc gcggcgcagc ttgtagagct ccgagccacc
    16141 cggaatggtg tactccacag ccacgaacaa ggccgcgatg agcggaatca gctccagcac
    16201 cagcacccgc accacggcgt ccagcgcgta ctgggtcagg ccgtaggaga aagcggtgac
    16261 caccaggatg cggatcagca ccacgttgaa cagtgccatc agtgtggtga agcccggcag
    16321 acccggcccg gcggcctgcc agatgtgacg cagcacggcc gagcgcgcct cgggcccgta
    16381 gctggaaggc gagaacgcca gcaccgccac ctgggcgccg gtgaacagca ccgtccacca
    16441 acccagcagc cagcgcagca gcccctgccc cagcccggcc agccagccgt acaaggaaaa
    16501 cccggcgctc atggcccgca tgatacccac gccgccccct cgccgaccgc ctgcccgcgg
    16561 ggcccggcgg cgtgtcgccg gcagcgtgtc gcgcacgcga cgcgaggggc gcaaacggct
    16621 cacccacgcc ctgcgtttta ggggctgcac accatgcaac ggacgcgcgg ttgcgctata
    16681 ttgcagtgca acacaggggt tacaactcag cctatgctct accagatcta tgaagcgcag
    16741 cgctcgctgc tcgagccctt cgccgacatg gccgatgcgg cctccaagct ctacggcaac
    16801 cgccacaccc tgctgggcca gatgccgatg gcgcagcgcg tgtcggccgg ttttgcgctg
    16861 tttcaccgcc tgggcaagga ctacgaaaaa cccgaattcg gcatccgcac cgtcgacgtc
    16921 gacggcgtgt cggtggcgat cgacgaacgc atcgagatcg acaagccctt ctgcgagctg
    16981 cgccgcttca agcgcttttc ggacgacccg ccgacgctgg acaagctcaa gcgccagccg
    17041 ccggtgctga tcgtcgcccc gctgtcgggc cactacgcca ccctgctgcg cgacaccgtg
    17101 cgcaccatgc tggagggcca caaggtctac atcaccgact ggaagaacgc gcgcatggtg
    17161 ccgctgtcgg agggcgaatt ccacctcgac gactacgtca actacgtgca ggagttcatc
    17221 cgccacctgc agcgcaacta cggcaactgc cacgtcatca gcgtctgcca gcccaccgtg
    17281 ccgacgctgg cggcggtgtc gctgatggcc tcgcgcggcg agaagacccc catcaccatg
    17341 accatgatgg gtggcccgat cgacgcccgc aaatcgccca ccgcggtgga cgatctggcg
    17401 gtcaagcgca gcttcgagtg gttcgagaac aacgtcatct accgcgtgcc gcccaactac
    17461 cctggcgccg ggcgccgcgt gtacccgggc ttcctgcagc acgccggctt catcgccatg
    17521 aacccggacc gccacgccag cagccactac gactacttcc gcgacctgat caagggcgat
    17581 ctgtccagcg ccgacacgca ccgcaagttc tacgacgagt acaacgccgt gctcgacatg
    17641 gacgcccctt actacctgga aaccatccag accgtgttcc aggacttcaa gctggtcaac
    17701 ggcacctggg acgtgcgctc gcccgagggc aagctcgagc gcgtgcgccc gcaggacatc
    17761 accgccacct gcctgctgac ggtcgagggc gagctcgacg acatctccgg cgtcggacag
    17821 accaaggccg cgcaccgcct gtgcaagggc attgcgcgcc gcgagggcga ccacatggag
    17881 gtcgaaggcg ccggccatta cggcatcttc agcgggcgcc gctggcgcac cgtggtctat
    17941 ccgcagatca aggccttcat cggtgaattc catgccctgt cgggactgcc gccggtattc
    18001 gcgccggcct gctcgccggc gtctcagccc gagccggcac cgacccagcg caagctgaac
    18061 ggccagcgcc ccccggccaa ggcgccccgg caggctggtc agggggccgc cgtgccaccg
    18121 gccccgcgcc cacgtgccgc agccctgccg gcggccagcg ccaccgccgc cggccggcgc
    18181 ggcaagagtg cccgtgtcgg ctgaacaccg gctgcgccct gcccaagacg cccgccctgg
    18241 cgggcgttgt cgtttgcggc gcacccgccc atgagcgcac cccccgcccc cgccgacgat
    18301 ctggccgccc gcctgctgga cgcgctgccg cagacgcagt gcacgcgctg cggcttcccc
    18361 gactgtgccg cctacgcccg ggccatggcc cgtggcgaag cgccggtcaa ccgctgcccg
    18421 ccgggtggcg ccgaaggcgt ggcgcggctg gcggccctca gcgctcagcc ggtgcaggcg
    18481 ctggatccat cgtgtggcag cgaaggcccg cgcggcgtgg cctggatcga tgaggactgg
    18541 tgcatcggct gcacgctgtg catccaggcc tgcccggtgg actgcatcct gggcagccac
    18601 aagcgcatgc acacggtgat cgaggccgaa tgcaccggct gcgaactgtg cgtgccggcc
    18661 tgcccggtgg attgcatcca catggacaac gtcagcggca gccgaaccgg ctgggccgcc
    18721 tggagtgcgc aacaggccca ggcggcccgc aagcgctatg aattcacgag ctcgcgccgc
    18781 aggcaggaca agggcaaaag ccggatttca tcaggatctt cgagcaccgc gccagacggt
    18841 gccccggccc tgcccccggc cgcgccggcg ggcgaggccg ccgaggccga gcgcaagcgc
    18901 cgcctgatcg aggccgcgct ggcgcgcgca cgccagcagc gcccgccgcg ctgaaccggc
    18961 ggtccggcgc aggctcagcg cagcgcggcg ttgatgcgtt cgagcaccgc cgcgcccggg
    19021 cacagatcgg ccacatccag gctgttgagc gggcgcgcgc tggtgtgctg ggcggcgtgc
    19081 agatcgtcgg cctcgccttc caggtacagc agcccggtca ccagctcgcc ccgggccgca
    19141 tgcgcctgca tgtaggccat ggccgccacg cggtcggtcg ggtcgtagtc ggcatgcagc
    19201 ttgtgcaggc gcagcacgct gccgtcgtgc tgctcgactt cgaccgtctg cccgggggcg
    19261 atggtggcgg tgattgcctc gcgcccggtg atgaagtcga tgcggctgac ggcctcgttg
    19321 tgctcgcgca cccagtcgta gctgcgggtg gagccggcgt ggttgttgaa cgtcacgcag
    19381 gggctgatga cgtcgatgaa ggcggcgccg ccatggccga tggcggcctt gatcaggggc
    19441 accagctgcg ccttgtcgcc cgagaagctg cgcgccacga agctggcgcc cagctgcatc
    19501 gccaggccca ccagatcgac cgggctgtcg gcattgacca cgcccttctt gcttttgctg
    19561 cccttgtcgg cggtggccga gaactggccc ttggtcaggc cgtagacacc gttgttctcg
    19621 acgatgtaga ccatgttgac gttgcgccgc atggcgtggg cgaactggcc caggccgatg
    19681 gaggccgaat caccgtcgcc ggagatgccc aggtacagca ggtcgcggtt ggccaggttg
    19741 gcgccggtca gcacgctggg catgcggcca tgcacggtgt tgaagccgtg gctggcgccc
    19801 agaaagtagt ccggcgtctt ggacgagcag ccaatgcccg acagcttggc cacgcggtgc
    19861 ggctcgatgt ccagctccca gcaggcctgc acgatggccg ccgacaccga gtcatgcccg
    19921 cagccggcgc acagggtgga caccttgcct tcgtagtcgc gccgcgtgta gccgaccttg
    19981 ttgcgggtga gcgaggggtg gtgcaggcgc ggcttggcga tgtaggtcat gtcgggttct
    20041 cctgcggggc gaccaccgcc tcgtgcacgt ggtcggtgat ggcctgggtg atgaagcgcg
    20101 cggtgatcgg cgtgccgtcg tagtgcagca cgcggcgcag cttggcgggg ttgatctcca
    20161 gttcgttgat cagcaggctg cgcatctgcg cgtcgcggtt ctgctccacc acgtacaccg
    20221 tctcgtgcgc gtcgatgaag gccttgacgg tatccggaaa gggaaaggcg cgcaggcgca
    20281 gggcgttgat gtggatgccc tgcgtggcca gctcgtccag cgcctcgtgc atggccgggc
    20341 tggtggagcc gtagaagatc gcgcccagtc gggtgcgcct ggccgccggg cgcggcaccg
    20401 gctgcggcac cagggtggcg gcggtggcga acttcttcag caggcgctgc acgttgtaga
    20461 tgtagtccgg cccgcgctcg gaatagcgcg cgtaggcgtc gcgcgtggtg ccgcgcgtga
    20521 agtagctgcc gcgcgtgggg tgggtgcccg gcagcgtgcg ccagggaatg ccgtcgccgt
    20581 cgacgtcctt gtagcggccg aagtccttct gctccaggtc ttcggcgctc atcaccttgc
    20641 cgcggtcgta gcggcgcgcg tcgtcccaga cgaggggttc gcacaggcgc tggttcatgc
    20701 cgatgtccag atcggtcatg acgaacaccg gcgtctgcag ccggtcggcc aggtcgagcg
    20761 cgcaggcggt gtgctcgaag cactcgcgcg ggtcttcggg cagcagcagc acgtgcttgg
    20821 tgtcgccgtg cgaggcgtag gcgcaggcca ggatgtcggc ctgctgggtg cgcgtgggca
    20881 tgccggtgga ggggccgccg cgctgcacgt tgatcagggt gaccgggatc tcggcaaagt
    20941 aggccaggcc gatgaattcg gtcatcagcg agatgcccgg cccggaggtg gcggtgaagg
    21001 cgcgcgcgcc gttccagccc gcgcccacca ccatgccgat cgaggccagc tcgtcctcgg
    21061 cctgcacgat ggcgtagttg tggcgaccct cggcgtcgcg gcgcagcttg gcgcagtagc
    21121 tcatgaacgc ttcgaccacc gaggtcgagg gcgtgatcgg gtaccaggca cacacggtgg
    21181 cgccgccgta caccgcgccc agaccggcgg cggtattgcc gtcgatgaag atgcgctgac
    21241 ccacccgatg ggcccgctcg acgcgcaccg gcagcggatg ccgcaggtgc tcgcgcgcga
    21301 agtcgcgccc caggttgagc gcctgcacgt tggcggcgat cagcttttcc ttgcctttgt
    21361 actgttcgcc cagcagctgt tcgatgacct gcgcgtccat gtccagcagc accgaaagcg
    21421 cgcccacgta catgatgttc ttgaacagct ggcgctggcg cggatcgctg taggtggcgt
    21481 tgcagatctc ggtcagcggc atgccgatga cggtgatgtc gtcgcgcagc tcggacggcg
    21541 cacgcgggcg cgtgctgtcg tacagcaggt agccgccggg ggcgatttcc tcgacgtcgg
    21601 cggcccaggt ctgcgggttc atggccacca tcatgtcgat gccgccgcgc cggcccaggt
    21661 agcccttttc gctcacccgc acctcgtacc aggtcggcag gccctggatg ttgctgggga
    21721 agatgttgcg cgggctgacc ggcacgccca tgcgcaggat ggccttggcg aacagctcgt
    21781 tggcgctggc cgagcccgag ccgttgacgt tggcgaactt gatgacgaag tcgttgatgg
    21841 cggcgatctg cttcatgccc ttacctcctg cgccgggtgc acgcccaggc tgtccgacgc
    21901 gccccctgcc ttggcggtga acagcaggaa cttctgcatg tcccaggcgc cggtcgggca
    21961 gcgctcggca cacagcccgc agtgcaggca gacgtcttcg tccttgacca tcacgcggcc
    22021 ggtcttgacc gcgggcgaca ccagcagcgc ctgctccggg ttcagtgccg gcgcgttcag
    22081 gcgctggcgc agttcggcct cgtcgccgtt gtcggtgaag gtgatgcagt ccatcgggca
    22141 gacgtcgacg cagccgtcgc attcgatgca caggggggcc gaaaacaccg tctgcacgtc
    22201 gcagttgagg cagcgcgaag cttccttgaa ggcggtgcgc gggtcatagc ccagctccac
    22261 ctcgacgcgg atgctggcca gcgcctgctc ggccttggcc cagggcacct tgtagcgctg
    22321 gtcgttgctg gggtcgttgt cgtagctcca ctcgtggatg cccatcttct gcgacaccag
    22381 gttcgcgcgc gggccggggc gccgggtcac gtcctggccg tccaggaaca ggtcgatcga
    22441 cagcgccgcc tcgtggccct gcgccacggc ggtgatgatg ttcttggggc cgaaggccgc
    22501 gtcgccaccg aagaacacgc gcgccagcgt ggactggaag gtgttctggc ccagcaccgg
    22561 caggccccag cggtcgaact cgatgcccag atcgcgctcg atccagggaa aggcgttttc
    22621 ctgcccgacc gccaccagca cttcgtcgca ggcgaagaag gcgtccggct ggccggtggg
    22681 caccaggctg cgccggccgc tggcgtcgta ttcggcgcgc acgatctcga acgtcatgcc
    22741 ggtcagcctg ccgccttcgt gcacgaaggc cttgggcacg tgcatgttga tgatcgggat
    22801 gccttcgtgc tgggcgtcct ccttctccca gggcgaggcc ttcatctcgt cgaaacccga
    22861 gcgcacgatg accttgacgt cttcgccgcc cagccgccgc gccgaacggc agcagtccat
    22921 ggcggtgttg ccgccgccca gcacgatgac gcgcctgccg acgctggtga catgaccgaa
    22981 cgacaccgag gccagccagt cgatgccgat gtggatgtgg gccgccgcct cgcgccgccc
    23041 cggaaggtcc aggtcgcgcc cgcgcggcgc gccagtgccg acgaagatgg cgtcgtagcc
    23101 ttcgctcagc agcgccttca tggagtcgat gcgctggccc gccctgaact gcacgcccag
    23161 gcggaagatg tagccggttt cctcgtcgat cacgctttcg ggcaggcgaa agcgcggaat
    23221 ctgctggtgc atgaagccgc cggccttggc ctcgccctcg aacaccgtga ccgcgtagcc
    23281 cagcgtggcc aggtcgcgcg ccaccgtgag cgaggccggg ccggcgccca cgcaggccac
    23341 gcgccggccg ttggcggaca ccggcgtggg catgcgcggc agcacgtcgt ccttgaagtc
    23401 ggccgccacg cgcttgaggc ggcagatggc caccggctcg ggcttgccgg tctgcgcctg
    23461 ttcgacccgg ccgcgccggc aggccggctc gcacggacgg tcgcaggtgc ggcccaggat
    23521 gcccgggaac acgttggcca cccagttgac catgtacgaa tcggcgtagc gcccctgggc
    23581 gatcaggcgc aggtattcgg gtaccggtgt gtgggccgga caggcccact gacagtcgac
    23641 caccttgtgg taataggctg gatcgcgggt atcggtcggt tgcaagcgcg gtctccttgg
    23701 gtggcgaggt ggcccgaccg ggccggtttg tatcgccacg taagtctacg cccgtcgggc
    23761 ccgcttccca accgaatcac cccccgccac aagtgcccat gcggcattgg cgtcacaccc
    23821 aaggaccgcc acggcagcta tcgttacgat agcttctggc gcttgtccag cggtctctaa
    23881 cggcataaaa cgttggcatc cagcgcggtg ccattgcgcc aggcgtcctt gctctggcgc
    23941 gtcgtctcgc aggcacctcc gggcagcagc aagggtccga ggacgcccca cggcggtgcg
    24001 cgcgccgcgc tttgcaccgt agcggtgcgc ccgcaccgtg acgacgcgcc gatgccatgc
    24061 cgccctgccc cggacccggc cgcatcaagg tgcgcttgga cgatggcacg gtatctgctt
    24121 tatccctgga aaccactgat gcttgctgcc cgcgccgacg gtccgatgcg accgttcggc
    24181 gcacagcgac gaaagacccg ccgatgagcg aagcagttgc cgaaaaaacc atctccgaac
    24241 gcgccttcaa cgaaatgctg gacgagggcg gccaagtgcg accgcattac cgcgcgtttg
    24301 cccagtggct cgcggcccag cccccacagg ccatggcggc gcggcgccta gaggccgacc
    24361 tgaacttccg gcgcataggc atcaccttct cgatggcggg cgacgccgac ggcaccgagc
    24421 gcctgatccc gttcgacctg ataccgcgcg tcatcccggc ggccgaatgg cgctggctgg
    24481 accgggggct caagcagcgg gtgcgcgcgc tcaacatgtt catccacgac atctaccacg
    24541 accacgacat cgtgcgcgcg ggcatcgtgc cgcccgagca ggtgttcctg aacgcccagt
    24601 accgccccga gatgcaggac gtcaccgtgg ccgagaacat ctactgccac atcgcgggca
    24661 tagacctcgt gcgcgccaac gcgggcgagt tctatgtgct ggaggacaac ctgcgcgtgc
    24721 cctcgggcgt gtcctacatg ctcgagaacc gcaagatgtc gatgcggctg atgcccgaga
    24781 tgttcagccg cgagcgcgtg gcccccgtgg cccattaccc cgacatgctg ctcgacaacc
    24841 tgcgcgaggt cgccccgcgc aacatcgacg accccacggt ggtggtgctc acccccggca
    24901 tgtacaactc ggcctacttc gagcacgcct tcctcgcgca gcagatgggc gtggagctgg
    24961 tcgagggcaa ggacttgttc gtggaccaga acacggtcta catgcgcacc acgcgcgggc
    25021 cgcagcgcgt ggacgtgatc taccgccgcg tggacgatga ttttctcgac cccctgacct
    25081 tccgcggcga ctcggcgctg ggcgtgccgg ggctgctgtc ggcctgccgg gcgcggcgca
    25141 tcacgctggc caacgccata ggcacggggc tggcggacga caagtccacc tatctgttcg
    25201 tgccggacat gatccgcttc tacctgggcg aggagcccat cctctccaac gtgcccacct
    25261 ggcagtgcag ccgccccaag gagctgtcgc atgtgctcgc ccacctgccc gagctggtgg
    25321 tcaaggaggt gcatggcgcg ggcggctacg gcatgctcgt cggccccacg gccagccgcg
    25381 ccgagattga agcatttcgc gcgcgcatcc aggccaaccc ggcccactac atcgcccagc
    25441 cgacgctggc gctctcgacc acgccgacct atgtcgaatc gggcgtggcc ccgcgccatg
    25501 tggacctgcg cccctatgtg ctgtgcggca aggagatccg taccgtgccc ggcggcctgt
    25561 gccgcgtggc gctcaaggag ggctcgctcg tggtcaacag cagccagggc ggcggcacca
    25621 aggacacctg ggtgctggaa gagtgacgca acgacaagag gaataccaag ccatgctgag
    25681 ccgcaccgcc gaccatcttt tctggatgtg ccgctacatc gagcgcgccg agaacaccgc
    25741 ccgcatgctg catgtcaaca cccagctggc cctgctgccg cgcgacgcgg ccgtgcatcg
    25801 ccagtcctgg caggccatgc tgcgcctgtt cgagctggaa gacgactttg ccgcacgcta
    25861 caacgacccc gagccccagg ccgtgatcca tttcatggtg cgcgacgccg acaacccgtc
    25921 gtcgatctac gcctgcctgc gcgcctgccg cgagaacgcg cgcgccgtgc gcggcagcct
    25981 gaccaccgag ctgtgggaga ccatcaacct cacctggctg acgctgctgc gcgacctcga
    26041 cgagggcgtg ctcgagcgca accccgagca attcttcgag tggatcaagc gccgcgcgca
    26101 cctggtgcgc ggcgtgaccg agaacaccat gctcgtcgac gaagccctgc acttcatgcg
    26161 cctgggcagc cagctcgagc gcgccgacaa catggcgcgc atgctcgacg tgcgctttca
    26221 cgagcacgag gccctggccg gcggcggcgc cccggccgcg cagggcaagg cgctcgatgc
    26281 cgatgcgggt gagttctacc gctggtccgc catcctgagc tgcgtgtcgg cgctggaggt
    26341 ctaccgcaag gtctatcgcg acgtggtcac gccctcgcgc gtggccgagt tgctggtgct
    26401 caacaccacg ctgccgagct cgctggtggc gctggtgcgc ggcgtgaggg agagcctggc
    26461 gcgcgtggcc aacgaccgct cggccgagac cgaacgccag gccggcctgc tgctggccga
    26521 gctcgagtac ggccgcatcg aggacattct gcgcggcccg ggcctgcacc gcttcttgac
    26581 gaacttcctc gcgcgcatca accacctggg cacgcgcata ggcagcgatt tcttgcttcc
    26641 tttgagtgcc tagcccttgt ggggcaagcg caagcagcta tcaaatttga gacaccgcaa
    26701 ggagccagac atgcgccatc tcatcaggca caccacccgc taccactaca ccgaccccgt
    26761 gagctacagc atccagctgc tgcgcctgac accgcggcgt gacgagcacc agcgcgtgct
    26821 gcgctggaac atcgaggcgc ccggcctgct cgaggaacag gtggacgcct acggcaacgt
    26881 cacccacacg ctcacgctga cccagccgca cgagaccata gagctcaagg tgacgggcca
    26941 gatcgacgtg gccccgctcg acgacggcgc cctgcccggc gaggacagcc gcctgccctg
    27001 gcaggcctat tgcgtgccca cgcagctcac ggcctgcgac gacagcattc gcgcctttgc
    27061 gcaggccacc ctgcccggcg gcctgcgcgg gcccgacgat gcgctggccc tggccgaggc
    27121 catagagcgg cgcgtggcct atgagcccgg catcaccgac gtgcgcaccg ccgccagcca
    27181 ggtgctggcc ctgggccatg gcgtgtgcca ggaccacgcc catctgttcc tggcctgcgc
    27241 ccgcgccctg ggccgccccg cacgctacgt gagcggctat ctgcacaccg acgccacgca
    27301 catggccagc cacgcctggg ccgacctgtg gtggcccgag cagggctggg tcagcatcga
    27361 cgtgaccaac cggcaatacg caagcgagcg ccactgccgc ctggccgtgg cgcgcgacta
    27421 cgagtcggcc tcacccgtgc gcggcgtacg caccggcggc ggcgccgaga ccatgctggt
    27481 cgacgtactg gtgcagcaat gagcggccgg gcgcctcata gaatcgcgcc ctcgggcgca
    27541 agccatgccg gcgcgtgcga ttactctgac ctcttgctat gacctactgt gttgccgcct
    27601 gcctggacgc gggcatggtc ttcctggccg acagccgcac caatgccggc gtggaccaga
    27661 tcagcacctg ccgcaagctg cgcgtgttcg agcgcccggg cgagcgcctg ctggcgctca
    27721 tgacctcggg caatctggcc atcagccagg ccatcgtggc cctgctggcc cagcgcggcc
    27781 cggacgaccc gcaatcgatc tggaacgccg ccaccatgca ggaggccgcg caatgcgtgg
    27841 gcgacgcagt gcgcgccgtg cacgcgcgcg acggccaggc gctcaaggag caaggcattg
    27901 acttcaacgt cagcctcatc ctgggcggcc agatcggccg cgcgccctgc cagctgttcc
    27961 aggtctatgc cgcgggcaac ttcatcgccg ccagccccga gggcccctac ctgcagatcg
    28021 gcgaggccaa gtacggcaag cccatcgtgg accgcgtgct gcaggcccac accccgctgg
    28081 acgaggccgc caagtgcatg ctgatctcca tggattcgac gctgcgctcc aacatctcgg
    28141 tcggcctgcc gctggacctg ctggtctatg agcgcgatgc gctgcgcgtg acccgctttg
    28201 cctgcctgga cgaggacaac cagtacctgg acatgatccg ccgcacctgg ggcgagcgcc
    28261 tgcgccaggt gtttgccgag attcccaacc cacagtggac cacgcccgag gcgcaggccg
    28321 gcgacgccgc cctgccctta tcgcgccgcc accagcccgt gcgcatcgcc gccggacccg
    28381 agccgcaggg ccagccgccc gcggtgcacc aggtgctcgc gcagggggcg ggcaggcggc
    28441 aggtgcagta gacgggtaca gcaggcgggc ggccgcagta aggaaaaaag ccccggccgt
    28501 ctccgaccgg ggctttttgt tgccgatgtc acgctcagcc gagggcggcg atcacctcgg
    28561 gcggtgcctg caccagctcg atgagcacgc cctcgcccgc gatcgggaac tcgtcgctgg
    28621 ccttgggatg catgaaggtg atgtcgtagc ccgccgcgcc cttgcggatg ccgcccggcg
    28681 caaagcgcac gccctgggcc gtgagccatt ccacagcctt gggcaggtcg tcaatccaca
    28741 ggccgatgtg gttgagcggc gtggtgtgca cggccggctt cttgtcgatg tcgagcggct
    28801 gcatgatgtc gacctctacc ttgtgggcac cgctgcccat ggcgaggatg tcctcgtcca
    28861 cgttctcgcg ctcactctgg aactggccgg tctgcgtcag gcccagcatg tccacccaga
    28921 ggcgcttcat gcgcgacttg tccgtgccgc cgatggccac ttgctggatg cccagcactt
    28981 tgaagggtcg ggtcatgaaa cactcctagt taactatgat ttttgtagct gccggcgcat
    29041 gccaggtaag cgccatggca ctattcattc agatgtctgg cctgcacgcg ctgcaccccg
    29101 gcggccgcca tgtcggccca ggcgcgcgcc agcgagccat cgaggtcgat cgcgcggcag
    29161 gcgtcctcga tcaccgtgac ctcgaagccg gcagcgcgtg cgtccagcgc gctccaggca
    29221 acgcagtagt cggtcgccag cccgcacaag aagaggcgct ggatgccgcg tgcgtgcagg
    29281 taggccgcca ggccggtgct ggtgcggcgg tcggcctcga cgaaggccga atagctgtcg
    29341 atatcgcgcc gaaagccctt gcggatgatg agctgggcgt ggtcgacccg caggtcgtgg
    29401 tgcagcgccg cgtcgcgcgt accttgcacg cagtgggtgt gccacagcac ctgcacgcca
    29461 tagggcagcg cgatggtctc gaacggctgg cgtccggggt ggttggcggc aaacgagaca
    29521 tggtcggccg ggtgccagtc ctgcgtcagc accaggttgg caaagcgcgg cgcgatgcgg
    29581 ttgatgaccg gcaccacggc gtcgccatcg gccaccggca ggccgccacc gggcatgaag
    29641 ccgtactggg catcgacgac gatcagcgcg gtctgcgggt tcatggcttc attgtgccgc
    29701 cggccgcagg gccggcagca actcgcgccg gatcagcgcc atcgcctggc cgcgggctgc
    29761 gctttcgggg gtacggcggg cggtgacggc aatcaccgcg tcgcgctgcg gatcgacgta
    29821 cagccactgg ccgccatagc cgctggccat gacggcgccg cgcgtgtgcc cgcgcgccgg
    29881 ccgctcgggc gacacccacc acaggtagcc atagggcgcg aacaccggcg cgccgccggc
    29941 gttgcgccgc gtggtggcct ggcgcaggta gtcggccggc agcaacgccc gcccgcccca
    30001 gcggccttcg gccagcacca gctcgcccag ccgcgccagg tcgcgcacac cgatcgacag
    30061 acccagcgcc cccagcgcct gcccctgcgc ccccggcgcc aggccaggcg cgtgatgccc
    30121 aggggcgcca gcagccgctc gcgcgcgtac tcggccagcg gctggccgac cgcgttggcc
    30181 agcgccagcg ccagcaggtt ggccgcgccg ttgtcgtagg caaagcgcgc ccccggctcg
    30241 gccacgaagg ggcgcagggc cagccagcgc aggtcgtcgt cgcggtcacg ccgcgccgtc
    30301 agctcgccgg gccagccggc cgtcatgctc agcaaatggg caaagcgcag ccgtcgcagg
    30361 cgcgcgtcgc cacccagccg caacaggcgc ggcaggcgct gcgccaccag ttcgtcgacg
    30421 ccgcgcaccc gcccgtcggc cagcgcgcag ccgaacagca gcgccagcac gctcttggtg
    30481 accgactgca cgtcctgcag gctgtcggcg ctgacgccgg cgcgatagaa ctcgaaacgc
    30541 accccacccc gctgcagcgc gaccacgctc tgcacgtcgg gccatgcggt ggccatgcgc
    30601 gcctgcaggc cggccagtgg gggcggcacc tgggcccgca ccccggccgc accgacgacc
    30661 cccgcaccca gggccaggac ggtgcgccga cggcagcgca aattcatatc aaacaggcct
    30721 gtagcgcttg tcagacttgc gccagaagct attattttag tagccccctg attgcccatc
    30781 ggcaccccct gccagccact gcgcgatctg cgcctgggcg gcggccagcg ccgccgcgtc
    30841 gtcaaaccgc acgcccagca ccttctgtcg cccgctggca ccgtgcagca gctcgacctg
    30901 cgaccgcggc acacccaggg cggccgcggc ccaggcgatc agcgccgcat tggccttgcc
    30961 gtcgaccggc ggcgccgcca gccgcacgcg cagtgcctgg ccgtgggccc ccgccgcttc
    31021 ggtgcgccgc gcgcccgggg tggcgtgaat cggcagcttc agcaggtgca cggccggcgc
    31081 gccggatcac tcgaactcga cgatcgcctg gtccaccgcc aggctttcgc cctgctgcgc
    31141 cttgacttcc ttgaccacgc cgtcggcgct ggccagcagc acgttttcca tcttcatggc
    31201 ttcgatcacc gccacgcgct cgccggcctg caccttctgg cccggctgca ccgccacctg
    31261 caccagcaga ccgggcatgg gcgaaagcac gaacttgctc atgtcgggcg gcgccttgta
    31321 gggcatcagc cggtgcagct cggcggtgcg cgggtgcatg acgatggcgt cgagctgcgt
    31381 gccgttgtgg atcacccggg tgaccagcgg gttgcgcgtg tggccgcgct ccatctgcgc
    31441 cgtgaagcgc tggccgttgc attcgccggt gatgcgcgac tgtcccaggt agcggtcgag
    31501 cttgagctcg tacacccgct cgccggcggc gccgcgcacc accacgcgcg tgggttcgtc
    31561 gcgctgcacc tcgcccaggc tgacatccac atactggtgc tggccctcct tgcccagggc
    31621 gcagacggtg aattcgcggc tgggcaggga cttgaaaccc tcccagaact gaccctgcag
    31681 gccctgcgcg cggttgcggt agtacatgtg caggcgcgcc gccagcgcga gcagaaagtc
    31741 ggggtccgcg tgcggcacgt cggcggccga gaagccgtgc gcgtagtgct cggcgatgaa
    31801 gccggtgttg aagtccccgg ccacgaagct ggggtgcgcc agcagcgcgg cctggaacgg
    31861 aatgttgctc tgcacgccct tgatgacgaa gccgttgagc gcctcgcgca tgcgcgcgat
    31921 ggcgtcctgg cggtccttgc cgtgcacgat cagcttggcg atcatcgagt cgtagtacat
    31981 cgggatctcg ccgccgtcga ccacgccggt gtccacccgc acgccgtagc gatgctcggt
    32041 gtcggcttgc cacatggtct cggccggcgg catgaagcgc accagccgac cggtggaggg
    32101 caggaagttg cggaacgggt cttcggcgtt gatgcggcac tcgatggccc agccgtcgcg
    32161 cttcacatcc tgctgggtga tgggcagctt ctcgcccgcc gccacgcgga tcatcagctc
    32221 caccaggtcc aggccggtga tgcactccgt caccgggtgc tccacctgca ggcgggtgtt
    32281 cat
//
LOCUS       DAOQTZ010000017        31816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1647_length_31816_cov_13.475237, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000017 DAOQTZ010000000
VERSION     DAOQTZ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 31816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31816
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1647_length_31816_cov_13.475237"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..572
                     /locus_tag="PKN51_03585"
     CDS             357..572
                     /locus_tag="PKN51_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HNT84213.1"
                     /translation="MSAPAATNSYRCDGRTHCSQMSSCEEATFFLKHCPGVKMDGDGN
                     GIPCERQWCSADKEQEHGQERWRGRRR"
     gene            900..1127
                     /locus_tag="PKN51_03590"
     CDS             900..1127
                     /locus_tag="PKN51_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84214.1"
                     /translation="MRRFWTGLLWALIGYVAGAFGGGLLVSLLSSNSHDRSLEAAMTG
                     AFVLGPLAAVVAFAVGYVRAGRRQTGRETEG"
     gene            1255..1848
                     /locus_tag="PKN51_03595"
     CDS             1255..1848
                     /locus_tag="PKN51_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84215.1"
                     /translation="MAEQITVAPQGVYAEIDTRLAIDTLRTLQTGSPAQITAAMDAIK
                     AAPERYAPPVFYVLSHVLFDRGDRDDAAFWFYAGQLRARFDANRCADVSARQAVAVLN
                     QTYGKPINQYTFKNIPKLEALIPRVVDWDRKTPHRYDHRWINLHGMGAMISGTGSPKQ
                     PPSLSLPADQWAPIAEKTRADYLEGFRQAMAQMKSRE"
     gene            2093..2497
                     /locus_tag="PKN51_03600"
     CDS             2093..2497
                     /locus_tag="PKN51_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNT84216.1"
                     /translation="MKQTYKGSCHCGAVRFEADIDFEAGTAKCNCSICTKARSWGAIV
                     RPDAFRLIHGESELSDYQFGPKVVHHYFCKHCGIRPFERGFHESVGGEFCSVQVSCLD
                     DLHIPDIFTGEVRHADGRNDNYLQSPSETRHL"
     gene            2702..3025
                     /locus_tag="PKN51_03605"
     CDS             2702..3025
                     /locus_tag="PKN51_03605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNT84217.1"
                     /translation="MNHQDTAKPIRKLARITAKPGHGDQLRAALLELEAATRQELGCI
                     EFSFFQAISDPDAFVLLEHFADEHAFKLHMTLEHTRSFFGTNAERVAGIKAVDIPSFE
                     SPVTR"
     gene            complement(3087..5375)
                     /locus_tag="PKN51_03610"
     CDS             complement(3087..5375)
                     /locus_tag="PKN51_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNT84218.1"
                     /translation="MSTVATLPLRAPALTGYKPFWAKRFGPAPYLPMSRAEMEALGWD
                     SCDVVIVTGDAYVDHPSFGMAVIGRVLEAQGFRVGIIAQPDWHSSEPFKALGRPNLFW
                     GVTAGNMDSLINRYTADRKIRSDDAYTPGDVAGKRPDRAAIVYSQRCREAYKDVPILL
                     GGIEGSLRRIAHYDYWSDTVRRSIVVDSKCDLLLYGNAERALVEIAHRLARREPVGQI
                     TDVRGTAFVRRTAAPGWREIDSTEVDRPGRVDALSSPYQTTAERAAAQGSHCDATKTE
                     AAGADPTSAAAQKGLEKPIALIRPDHARSVIRLPSYEQVRRDPVLYAHANRVLHLETN
                     PGNARALVQAHGEGRSARDVWINPPPVPLTSAEMDHIFDLPYARNPHPRYADERGRHD
                     GATRIPAWEMIRFSVNIMRGCFGGCTFCSITEHEGRIIQSRSEDSIVREVEAMRDKVQ
                     GFTGVVSDLGGPTANMYRLGCRDPAIEAVCRKPSCVYPGICPNLRTDHDPLIRVYRRV
                     RQLPGVKKVLIGSGLRYDLAVRSPAYVKELVQHHVGGYLKIAPEHTEQGPLSKMMKPG
                     IGDYERFRQLFDRFSAEAGKQQYLIPYFIAAHPGTSDQDMLQLALWLKRNGFRADQVQ
                     AFYPSPMATATTMYHTGVNPLKGVHRDGRGERVDTVKGERRRRLHKAFLRWHDPANWP
                     LLRQALKDMGRHDLIGSGKHHLIPAWQPAGSPAYQSPRRKNSSPAASPRKLQKQELPA
                     RTQPAPGRILTQHTGLPPRRST"
     gene            complement(5581..6105)
                     /locus_tag="PKN51_03615"
     CDS             complement(5581..6105)
                     /locus_tag="PKN51_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HNT84219.1"
                     /translation="MFRLPQPARAHVASIPAATRLAVAAALLAVLAGCASPPPAPRPR
                     ATPSAPPRLPELAAFSAQLSGRQAVPPSASTGQGELVAVLNRNTGLLQWKLSFSGLSG
                     PVRAAHFHSPGMAGEVADPVLTLGRTVVSPLEGRAMLTPRQRADLLSGQWYVNLRTAA
                     YPEGELRGQLIERK"
     gene            complement(6219..6848)
                     /locus_tag="PKN51_03620"
     CDS             complement(6219..6848)
                     /locus_tag="PKN51_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HNT84220.1"
                     /translation="MSLPHWNRSHAVLAGLVAAGLLSACASVDLGRRYDPPPIRMPQP
                     LPPAQVPPAQPGGVQTQPVPPAQPVPQPLPPAGQSAPLPPTDASARLVTLSTRLDGAS
                     VMPPTRSAGSGQWDAIYDPDTRLLRWKTRWSGLSGAITGVQFHGPADAGQNAPAVLVW
                     PAPFGPSYEGRASLSAAQAMDLLAGRWYVSVFTAAYPLGELRGQVRVVP"
     gene            7010..7429
                     /locus_tag="PKN51_03625"
     CDS             7010..7429
                     /locus_tag="PKN51_03625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNT84221.1"
                     /translation="MPTLDLPALQQLIQPLLPGLLGVRLTEATAERVRAEMTVRPELC
                     TTGHVLHGGSHMALADTLGAIGTVLNLRPGQRTVTTDSSTKFIAGAAEGSVVTAEAVA
                     LHRGRSTQVWQTTIRNAEGRLCAVVTQTQLVLATPGE"
     gene            7449..8009
                     /locus_tag="PKN51_03630"
     CDS             7449..8009
                     /locus_tag="PKN51_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HNT84222.1"
                     /translation="MSQPLSRHRLHPHPSTPAPAVRSLCVDVGRSDTAPGAWWLRFRL
                     QAALEALALPPPGASAGPADDLWRHTCFEAFVGRAQAAAYHEFNFSPAGDWAAYAFSG
                     PRQRASADQLPLAPRLQWQRADDTLTLTVELPRQALPAADHTDLLLGLSAVIEARDGQ
                     LSCWALAHPGPQPDFHHRAGWTARLP"
     gene            8020..9222
                     /locus_tag="PKN51_03635"
     CDS             8020..9222
                     /locus_tag="PKN51_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNT84223.1"
                     /translation="MKFGLERFLEEPLLRAPLAGRRVALLAHPASVTRNLTHALDALA
                     ALPDVRLTAAFGPQHGLRGDKQDNMVESSDYTDPRLGIPVFSLYGEVRRPTPAMLETF
                     DVLLVDLQDLGCRIYTFITTLRYVLEAAAAHGKAVWVLDRPNPVGRPVEGLTLRAGWE
                     SFVGAGPLPMRHGLTLGELGHWFIAQLGLQVEYRVIAMEGWRPDAAPGHGWPLERTWV
                     NPSPNAPNLWMARAYAGTVMLEGTTLSEGRGTTRPLELFGAPDIDTARLLADMRALAP
                     DWLRGCRLRECGFETTFHKHAGQLCAGVQIHTEDATHYDHTTFQPWRLQALAFKALRR
                     QAPDYPLWRDFPYEYETRLAIDVINGGPLLRQWVDDAAATPAELDALTRADEQAWRAA
                     RTPFMIYE"
     gene            9302..9655
                     /gene="arsC"
                     /locus_tag="PKN51_03640"
     CDS             9302..9655
                     /gene="arsC"
                     /locus_tag="PKN51_03640"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722955.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNT84224.1"
                     /translation="MATVTIYHNPHCSTSRKALELLRSRGIEPTIVQYLKTPPSAAEL
                     QAIARASGAPLRALVRTKQPEYLAQGLDDATLTDAQLAAALHATPVLIERPIVVTARG
                     ARLGRPVENILDLLD"
     gene            9753..10340
                     /locus_tag="PKN51_03645"
     CDS             9753..10340
                     /locus_tag="PKN51_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNT84225.1"
                     /translation="MSATPPAAAGDGTLGAPRSRADLFWSFSWLALQGFGGVLAVVQR
                     ELVERKRWLTREQFVEDWAVAQIMPGPNVVNLSLMIGDRHFGLSGALAALAGMLTLPT
                     LAVVLLAALYSNVADVPLVQGALRGMGAVAAGLILGTGIKLIGALRANPLGMPACIGL
                     ALATFVAIGLLRLPLGPVLLVLGGAACVWAWRRLP"
     gene            10337..10939
                     /locus_tag="PKN51_03650"
     CDS             10337..10939
                     /locus_tag="PKN51_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNT84226.1"
                     /translation="MNPADALSAADWLTLWLHFLSLSLLAVGGAITTAPDMHRYLVGS
                     QHWLSDAQFNASIAIAQAAPGPNVLFVALVGWHVGLNAGGGPAGGWHAQGLALLGAAV
                     AMLGILLPSGVLTYSATRWAHQRRELRAVRAFKAGLAPIVIALLMATGWLLSTAHGQP
                     ARDWPLWLLTAATTVLVWRTRLHLLWLIGAGALLGMLGWI"
     gene            complement(11199..12164)
                     /locus_tag="PKN51_03655"
     CDS             complement(11199..12164)
                     /locus_tag="PKN51_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT84227.1"
                     /translation="MNLDHGIHVIDTGFVRPHLDAAYLIVERGRGAFIDCGVNHSVPH
                     LLAALAQAGLTPGDVDWLILTHVHLDHAGGAGELLRQLPQARLVVHPRGARHMIDPSR
                     LWAGAAAVYGEAEMQRSYGRLLPVPAERVVAAPDGHVVELAGRPLVCLDTPGHARHHN
                     AIHDLASGCVFSGDTFGVSYREFDTEQGAFILPTTSPVELDPPALHRSIDRLLALQPP
                     AICLTHYGPVAPPARLAQDLHAQLDAMVELAHAAAPQPSDERHRSLRAGLLELYARRA
                     AQHGWRGGRERLAELLAVDIELNAQGLGVWLDQSTRMTKDPKNDS"
     gene            complement(12432..14084)
                     /locus_tag="PKN51_03660"
     CDS             complement(12432..14084)
                     /locus_tag="PKN51_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HNT84228.1"
                     /translation="MATPLTAQQLVLRLSLYLVLAANWPLWLQIMRIGGAPSQYLRSM
                     AALAVLIVCGMVAIFAFFAWARGMRLLWWLVVLVAALAQYYMLTYQVVMDPGMAANVL
                     QTDAREAADLLSLRMLLTVLAVLVLPSWWLLRVRIVAMGPLAQLWRNLVMLVLALAVA
                     AGTVAATARDLAPLMRSHPQLRYLMNPLASLYSTPVAALRPVFARSRTLVPMTQGAAL
                     GTSYAAGARPMLFVLVVGETARADHFGINGYGRDTTPELARRDVLSWREVRSCGTSTL
                     ASVPCMFSPLGKDAFESRDNEYENLLDVAQTAGMAVLWLDNQSGCKGVCDRVAHASAS
                     EALPRDAQAALCPGGECGDDALLHDLDARLAALPAAQRERGVLLVLHQMGSHGPAYHK
                     RSHGAAKRFLPECRTEVLADCAQQELVNAYDNSIAATDLFLGKTIDWLQARSARYDTG
                     LLYLSDHGESLGEYGLFLHGMPYNMAPDVQKHVPMVAWLDDALLRRERLSDACLRAGA
                     NAPLTHDNLYHTMLGLLDVQSPTYTRALDAFADCRRVALAAR"
     gene            complement(14233..14931)
                     /locus_tag="PKN51_03665"
     CDS             complement(14233..14931)
                     /locus_tag="PKN51_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNT84229.1"
                     /translation="MFDVLWRRLALVLLPLVPLLLWDASGLDLPLARLFGSAQGFAWR
                     SHPSYVLLLHEVPRALSMAALLALAWGAWRPWGFLRTMGAADRRQLAFSVALAMLSIT
                     ALKRLATTSCPWDLAEFGGVAHYVSHWIWGVRDGGPGHCFPAGHASAGFGYVAGWFVL
                     RRAVPRLATGWLLASLVLGCALGLSQQVRGAHYMSHTLWTAWFCWALGLLVDEAVQRW
                     RSRTRARDTNLNET"
     gene            15019..15690
                     /locus_tag="PKN51_03670"
     CDS             15019..15690
                     /locus_tag="PKN51_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT84230.1"
                     /translation="MRVLLVEDDLALSDAVCGYLRAKAFVVDATASLSEAEAALHGAQ
                     YAAVLLDLHLPDGDGLALLPQLRALQQRPIVIVLTARDQVSDRIRGLDAGADDYLIKP
                     YDPGELLARLRAVERRRSAAETPVLVLGDLQIDLAREQVRRASVPIALTQKEWALLRV
                     LATRPGRIHTRENLQDALYGWGDETDSNTLEVFISRLRRKLGRTHIETVRGLGYRLHS
                     EPAAA"
     gene            15687..17099
                     /locus_tag="PKN51_03675"
     CDS             15687..17099
                     /locus_tag="PKN51_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT84231.1"
                     /translation="MRASTLAQRLTRALVLRLALVWLLCVLAVAYYLNHEIEENFDAE
                     LAESAHRMFDVAMFQLQRGAPAGAAGQAPRADAVIADDPLFADAPLVYQLVDAQGRLL
                     LRSRQAPAQALAVPLEPGFHDTNQWRVHVAQHPEQALFFLLADPLSERNHEWRETLLV
                     LLVAAVLALAALAWALPGVVTRELGVLERLQRQIRRRGGGDLRPLTLTGLPAELRAVG
                     EDVNLLLQRLDQALDVERALAANAAHELRTPLATARLRLQTALEHGLHRADVQAAVDA
                     LRTLSQRTEKLLQLSRAESSAPLSREPVDLVRLAATVAQEFWQDERAADRLELITATD
                     GEHCTALGDFDALAIALRNLVENALHYAAPGPVEILVGPGPMLAVRDHGPGVSADRLK
                     SLQQRHVRQSADRAGYGLGLSIVATIAAKHDAQLTLTSPLPHESGGFEARMQWTVATP
                     PERASSRAAAPPSAPESPPR"
     gene            complement(17035..17166)
                     /locus_tag="PKN51_03680"
                     /pseudo
     CDS             complement(17035..17166)
                     /locus_tag="PKN51_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
     gene            complement(17548..17637)
                     /locus_tag="PKN51_03685"
     tRNA            complement(17548..17637)
                     /locus_tag="PKN51_03685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17601..17603),aa:Ser,seq:cga)
     gene            complement(17684..18994)
                     /gene="serS"
                     /locus_tag="PKN51_03690"
     CDS             complement(17684..18994)
                     /gene="serS"
                     /locus_tag="PKN51_03690"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNT84232.1"
                     /translation="MLDPLLLRKDLDGVVARLLTRKNPQPFLDVAAFQALEAERKAIQ
                     TRTEELQAQRNSASKQIGQRKAKGESADDLMAQVASIKDELDASAARLERIQDELQTL
                     LLAVPNLPHDSVPVGADEAANHEVRRWAPHGLAPRSFDFAVRDHVELGEPLGLDFETG
                     AKLAGSRFAFMRGPVARLHRALAQFMLDVQTGEHGYTECYTPYIVNAETLRGTGQLPK
                     FEADLFAAKKGGQEGEGEPLYLIPTSEVSLTNLVRGEILAEADLPLQLTAHTPCFRSE
                     AGSAGRDTRGMIRQHQFDKVEMVQIVHPERSYEALEQMVTHAEAILQKLELPYRVVLL
                     CTGDMGFGASKTYDLEVWVPAQNTYREISSVSNCEAFQARRMQARFKTAQGKNELVHT
                     LNGSGLAVGRALVAVLENHQNADGSIGIPRALQPYLGGIERLAP"
     gene            complement(19104..20150)
                     /gene="ispH"
                     /locus_tag="PKN51_03695"
                     /gene_synonym="lytB"
     CDS             complement(19104..20150)
                     /gene="ispH"
                     /locus_tag="PKN51_03695"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNT84233.1"
                     /translation="MPQSHGRSITITRKAAAADGEAAAAPPVDAHILLAEPRGFCAGV
                     DRAIEIVERALVKFGAPIYVRHEIVHNTYVVNDLKAKGAIFIEELAEVPPGATLVFSA
                     HGVPKAVEREAQARGFRVFDATCPLVSKVHVEVAKLHREGYEFIMIGHKGHPEVEGTM
                     GQLPGGIHLVEEVQDVARIRPTQTERLAVVTQTTLSVDDAAEITAAVKARFPMVREPK
                     QQDICYATQNRQDAVKLLSREVDVVIVVGSPTSSNSNRLREVALKLGTAAHMVDSADD
                     LKPEWVQGRARIGLTAGASAPDVLVRQVIERLRALGAVSVRQLAGIEETMKFPLPKGL
                     KLDSASEDGLAHLR"
     gene            complement(20150..20593)
                     /locus_tag="PKN51_03700"
     CDS             complement(20150..20593)
                     /locus_tag="PKN51_03700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT84234.1"
                     /translation="MSTVEPGAFLTLHYRLSGPQGDVINTFGGPPATLSLGAGELSPA
                     IEQRLIGLAEGARATFELPAGEAFGERSPDMIQWLARQDLDELGDPQERYAVGDVIQL
                     PTPDGQGQFGAVVQAVRDDGALRLDFNHPLAGQPVTFEVQVIGIL"
     gene            20650..21327
                     /gene="radC"
                     /locus_tag="PKN51_03705"
     CDS             20650..21327
                     /gene="radC"
                     /locus_tag="PKN51_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNT84235.1"
                     /translation="MSLKDLPADARPREKLLARGAAALSDAELLALLLRTGTAGRGVL
                     QMAQELLDRFGGIAGLLHASADDLGRFKGLGGSAKRAELIAVLELARRALAEQLKEKP
                     ALDSASAVKQYLQLHLGQRPHEVFAALFLDAQHRLVALEELFRGTLTQTSVYPREVVL
                     RALHHHAAAVVLAHNHPSGQVAPSRADEALTQTLKAALSLVDVRVLDHIIVAPGQALS
                     MAEQGLL"
     gene            21324..21965
                     /locus_tag="PKN51_03710"
     CDS             21324..21965
                     /locus_tag="PKN51_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNT84236.1"
                     /translation="MKARSLQDLKQLRTQLAEREARAAQAQAERAAAQQRARADQDLF
                     ARAIGATQPLPAHGRVVHAPPAVAPEPRQRQRDEQAVLRESISDEFDTSTLLHTDEHL
                     SFRRPGIGPDVAAKLRRGHWAVQGEIDLHGLRTDEAREALAQFLREAQRAGLRCVRVV
                     HGKGLGSPGRTPVLKAKVQVWLVQKAEVLAFVQAKPLEGGAGALLVLLAASIR"
     gene            complement(22151..23005)
                     /locus_tag="PKN51_03715"
     CDS             complement(22151..23005)
                     /locus_tag="PKN51_03715"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HNT84237.1"
                     /translation="MSFPHNADLHCHSVVSDGTLTPEQLAARARANGVDLWSLTDHDE
                     VGGQQRALEAARAEGLRYLTGVEVSVTFADETVHIVGLGFDHTDPRLAEGLADTRDGR
                     GPRAVEMGEQLARVGIAGAYEGALRYVGNPRLISRTHFARFLVETGVCRDTAEVFRRY
                     LVEGKPGYVPHRWARLGDAVRWITQSGGVAVIAHPARYDISPTVEYALFSEFKQHGGQ
                     AVEVITGSHTAAEAARYAAMAREFGLAASRGSDFHSPDESHTDLGQLPPLPADLTPVW
                     TLLANRIH"
     gene            complement(23070..24494)
                     /locus_tag="PKN51_03720"
     CDS             complement(23070..24494)
                     /locus_tag="PKN51_03720"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNT84238.1"
                     /translation="MTEPLHDLSAHALLAGYRARHFSPVDVLDSVLHHVERWEPHIAA
                     SWLLRPDAVRAQARASEARWRRGEPCGALDGVPVTIKDNIATAGDPVPLGTRAVPLLP
                     ATADAPPAARLREAGAILLCKTTMPDYGMLSSGLSTFHRLSRNPWDLALTPGGSSAGA
                     GAGAAAGYGPLHLGTDIGGSIRLPAAWCGIVGLKPSLGRVPIDPPYMGRAAGPMTRSV
                     QDAALLMQVLSQPDARDCMSLPPQAIDWSLAWRQDKPLKGLRIGLLLQAGCGLPVEPA
                     IRAAVEQAARLLEGQGAIVTPWAPFMTPELLAGLDHFWRMRSLLDLDALSAPARASVL
                     PVIRDWAESARGMSGADLFRAYAATHATRVATSQAMQGLDYLISPVSPNAPAPAEWPS
                     PTNDPLRGLEHIGFTVPFNMGEQPAISINCGYMASPATPGAMLPIGLQIAGQRFDDLG
                     VLQLARAFEQLRGPQRPWPAPPAP"
     gene            complement(24570..26057)
                     /locus_tag="PKN51_03725"
     CDS             complement(24570..26057)
                     /locus_tag="PKN51_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT84239.1"
                     /translation="MKRRDFALSLPALAASTHLPALAQAGKSTMVLGMTLEPPGLDPT
                     AGAASAIAEVVHYNILETLTKINPDGSVSPLLAERWEVSPDLRTITFYLRKGVKFSNG
                     EPFNAQAVRFSFTRAAGDKSTNKDKRTFAAMESVSAVDEHTVVIINKEPDPDFLFYMG
                     QATAVIVEPKSADGNNARPVGTGPYELDNWAKGSSITLKAWPGFRNASAIRIKRATFR
                     FISDPAAQVAAVLAGDVDAFPRVAPRGASQFKANPRLQTLITGSRAKTVLAINNARKP
                     LNDVRVRRAISMAIDRKAVIAGAADGYGVPIGSHYVPGAPGYVDTTGINPYDPEKAKA
                     LLKEAGVTTPLELSLVLPPPSYARQGGEVIVAQLAKIGITAKVQSVEWAQWLSNTFGG
                     PKNYDLTIISHVEPFDLGNFAKPDYYWGYQNPKFNELYTRIKNTPTAAERNKLLGDLQ
                     KLLANDAVLAWLFQPQGVTVANKRVQGLWKESPIFVNDLSSLSWG"
     gene            complement(26282..27076)
                     /locus_tag="PKN51_03730"
     CDS             complement(26282..27076)
                     /locus_tag="PKN51_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT84240.1"
                     /translation="MLETRELERRYTLPRESLFRTPGQVHALRGVSLQVTAGRSLGIV
                     GESGSGKSTLARLVMALERPTAGQVLLQGRDLHALSPDELRAARRDFQMVFQDPYGSL
                     DPRRRIWRTVAEPAAALEGADRAEQRERAAAMLTAVGLHADALDQYPHEFSGGQRQRI
                     AIARALITRPKLIVADEAVSALDVSVQAQVLNLMADLRDAHGVTFLFISHDLAVVAHL
                     CQDVAVMQGGRVVEHGPVRQVLREPRHPYTRQLLDAVPVSALLAAV"
     gene            complement(27100..28110)
                     /locus_tag="PKN51_03735"
     CDS             complement(27100..28110)
                     /locus_tag="PKN51_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT84241.1"
                     /translation="MALLQAEHLRVSLRTHRGSATAVRDVSFALERGDTLGLIGESGC
                     GKSMTALALMGLQPDNATVQGSLRLDGQELLGLPDRDWRRLRGNRIAMIFQEPMTALN
                     PVHRVGAQIAEPLRLHRGLSAQAARQRAIELMARVGIPEPARRIDSFPHQFSGGQRQR
                     IMIAMALACEPDVLIADEPTTALDVTVQQQILALIRELVAERGMALVLISHDLGLIAQ
                     NVRRMLVMYGGAVVESGPAAELFRQPAHPYTRGLLAARPNLAQRLHAHRSDRLPTIAG
                     SVPDLFGLPPGCPFAGRCAYTEPACLTDPAPPVALTASHRFWCRRADTVLHAPESIAG
                     GA"
     gene            complement(28115..28447)
                     /locus_tag="PKN51_03740"
     CDS             complement(28115..28447)
                     /locus_tag="PKN51_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84242.1"
                     /translation="MTLTVKSPPYLEQSLGQQSAAEGRSLSELWRDALTADLASARHA
                     HGDEVLAHKRAGRIARRFARMPLNPAGASRAEAAARRGLRRILGIDAACIVRRDSRGR
                     PLPNLLSS"
     gene            complement(28491..29366)
                     /locus_tag="PKN51_03745"
     CDS             complement(28491..29366)
                     /locus_tag="PKN51_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT84243.1"
                     /translation="MSGTLRLAAAVGAAPGFWARARRHPGFVIGALLTLALLLSAALS
                     YVWTPYSVYEVQMDDKLLGPDARHWLGTDAFGRDVVSLLLVGARASILVGVIAVGIGL
                     VFGAALGLLASARRGWVEELIMRVADFGFAFPAILSAIMLTAVYGPGIVNAIIAIGIY
                     GIPAFARITRAAANAVWSRDYVQAARASGKGAWAITWQHVLPNILAVLIVQATIRFAV
                     AILAEAALSYLGLGTQPPQPSWGRMLAEAQSLMFDAPLLAVWPGLAIALSVLGLNLLG
                     DGLRDVLDPRLQGSR"
     gene            complement(29530..30498)
                     /locus_tag="PKN51_03750"
     CDS             complement(29530..30498)
                     /locus_tag="PKN51_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT84244.1"
                     /translation="MSYFLFKRILTLLATLAATSVIVFAVLEVLPGNAAQVLMGPDAD
                     PAAVAAKVVELGLDRPPLARYVDWMRGLLRGDMGNSYAYGSPVAELIAERLLLTVPLA
                     VLAMTLTALLALAVGVYAASQHNRLGDTGLMALAQVGIAIPNFWFAILLILLFSVKLQ
                     WLSAGGFDGWYFDDGVWAGLWQGLKALILPAVALALVQAAILARFTRSAVLEVLREDF
                     VRTARAKGLSRRATLWGHVLRNAMIPVITVMGLQSAELLAGTIVVESVFYLPGLGRLI
                     FQSIANRDLIVVRNCVMLLAAMVVVVNFIVDVLYAVIDPRVKASEV"
     gene            30585..30836
                     /locus_tag="PKN51_03755"
     CDS             30585..30836
                     /locus_tag="PKN51_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNT84245.1"
                     /translation="MALMITDDCINCDVCEPECPNQAIAMGEEHYEIDPDKCTECVGH
                     FDQPQCVQVCPVDCIPVNPQRMEDPQQLLDKFRRLLGQA"
     gene            complement(30888..>31816)
                     /locus_tag="PKN51_03760"
     CDS             complement(30888..>31816)
                     /locus_tag="PKN51_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HNT84246.1"
                     /translation="IDPKMLEMSVYEGIPHLLAPVVTDMKQAANGLNWCVAEMERRYK
                     LMSKAGVRNLAGFNQKIDDAKARGEFIYNPFSLTPEDPEPLERLPYIVVIIDELADLM
                     MVVGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAFQVSSKID
                     SRTILDQMGAEALLGMGDMLYMASGTGLPVRVHGAFVSDDEVHRVVEYLKSQGEPNYI
                     EGVLEGGTAEGDAGAFGEGGDAGGEKDPMYDQAVEVVLKNRKASISLVQRHLKIGYNR
                     AARLVEDMENAGLVSAMTADGKRDILVPARPE"
BASE COUNT         4897 a        10961 c        11169 g         4789 t
ORIGIN      
        1 gccggtctca aacagttggg tcacaccagt gcggcctaac acatcattcc accggcctgc
       61 gcaaaaggcc gcgcaggccg gtgtattcag acgttgacac aaatatcatg cgagttactt
      121 catcgcgcca tctcttggta ttcgcaacgc tttgcgcggc cgccgggctg catgccgcgc
      181 cactgaacaa gtgcgtcatc aacggtagcg tttcctatca gcaagcgcca tgccctgcaa
      241 atcaggagca aagaaagcgg cccacactgg acgaactgaa cgccgcagaa aaacagcgac
      301 gcgcagcttc cagcgcagat agaaccacag gctcgcttcc ccccagagag gtgcctatgt
      361 cggcgcctgc agcaacaaac agctaccgct gcgacgggcg gacgcattgc tcacagatgt
      421 cctcatgcga agaggccacc ttcttcctca aacattgccc aggcgtcaag atggacgggg
      481 atggcaacgg tattccttgc gagcggcaat ggtgcagtgc cgacaaggaa caagagcacg
      541 ggcaagaacg gtggcgaggg cgccgacgct gataccagct ggtcacgcgc cagacattgc
      601 ctgagagggc atatatggag tccccatcaa cgtaaaggga atgaccgatt gagggcactg
      661 gggcgtggcg cacctgcgtt cgcaaaatcc ggcatctgtg gtgaactctt ttctggtccg
      721 tgccgacatg gaatccgcgc caccgcgtcg ccacacgttg caatcggcag acaagctgcg
      781 gcaagcgttt tcggcgtaga gcggtatggg agcagcccgg ttggtcatga tggtcgatca
      841 aatccttcgc aaactggacc ggagcggtca aaaatgcgat gaccagaaag cacccaccca
      901 tgcgcagatt ctggaccggc ctgctgtggg ccctcatcgg ctacgtcgcg ggggcatttg
      961 gcggcggcct gctggtgtcg ctgctgtcgt ccaacagcca tgaccgatcg cttgaggcgg
     1021 cgatgaccgg ggcctttgtg ctgggcccgc tggctgccgt tgtggctttt gcagtcggat
     1081 atgtgcgcgc cgggcgcaga cagaccgggc gcgagaccga aggctgaaac cgggcgatca
     1141 accccttcca gattcaaccc acgcccaggc aaccccatgc gccctcgccc actcccccgc
     1201 atgcctgcgg ccctgctggc cttgctgttt gcactgggcg ccggcgcgcc tgccgtggcc
     1261 gagcagatca cggtggcgcc gcagggcgtg tatgccgaga tcgatacgcg cctggccatt
     1321 gacaccctgc gcacgctgca aacgggcagc ccggcgcaga tcaccgccgc gatggacgcc
     1381 atcaaggcgg cgcccgagcg gtatgcgccg ccggtgttct atgtgctgtc ccacgtgctg
     1441 ttcgatcgcg gagaccggga cgacgcggcc ttctggtttt atgccgggca gttgcgcgcc
     1501 cggtttgacg ccaatcgctg cgcggatgtg tcggcgcgcc aggcggtggc ggtgctcaac
     1561 cagacctacg gaaagccgat caaccagtac accttcaaga acattcccaa gctggaggcg
     1621 ctgatcccgc gcgtggtcga ctgggaccgc aagacgccgc atcgctacga ccatcgctgg
     1681 atcaacctgc acggcatggg cgcgatgatt tcgggcacgg ggtcgccaaa gcagccccca
     1741 agccttagcc tgcccgccga tcagtgggca ccgattgccg agaagacgcg ggccgattac
     1801 ctggaaggct ttcgacaggc catggcgcag atgaaaagcc gcgaatgagc aatactggca
     1861 cccatcgagt tcgttcgatc ggaaagcacc caaccatggg caaattctgg cacccctggg
     1921 ctccagacca aagcgccaca aaaccttggg ggaagggcat gttccgactt tccatgctga
     1981 ccgtcggatt cgatggcttg ttcgccgcag tcggccgcgt gcgactgctg aacctcaggc
     2041 gttggtctgc acggccaacg cggtcgcttt gatacacaac gagaaagacg aaatgaagca
     2101 aacctacaag ggcagctgcc actgcggcgc agtgcggttc gaggcagaca tcgactttga
     2161 agctggcacc gccaagtgca actgctccat ctgcaccaaa gccagatcct ggggcgccat
     2221 cgtcagacca gatgcattcc gcctcattca tggtgagtcc gagttgtccg actaccagtt
     2281 cggtcccaaa gtcgtgcatc actatttctg caagcactgc ggtattcgcc cgttcgagcg
     2341 cgggttccac gaatcggtgg gtggagagtt ctgctccgta caggtgtcgt gcctcgacga
     2401 tctccacatt cctgacatct tcactggcga agtgcgccat gcggacggac gcaacgacaa
     2461 ctaccttcag tccccgtctg aaacccgtca cctttagtgc agctgccggc cagcatttcg
     2521 cgcacggtgc tcaaggtgct cacccgcact tgtgcattcg accgcccgtc agcctgacgt
     2581 ttgtccgtcc gcgccccgtc ctgcgcttga aataagctgg aacaaccccc actctcctgc
     2641 ctggcccatc cgcgcgctct tcaagctcgg ggagacgaac gaccgacgtt gagaaggttc
     2701 catgaaccac caagacactg ccaaaccgat ccggaagctc gcacgcatca ccgccaagcc
     2761 tggacacggc gatcaactgc gcgcggcgtt gctcgaactt gaagcggcca cacgacagga
     2821 gttgggatgc atcgagttct ccttcttcca ggccatttcc gatccagacg cctttgtgct
     2881 tctggagcac tttgcagacg agcatgcgtt caagctgcac atgacgcttg agcacacacg
     2941 cagcttcttt ggcaccaacg ccgaacgggt ggctggcatc aaggccgtcg acatcccgag
     3001 tttcgagtcg ccggtcaccc gatgaacccg tggcaaccca tgcccgtggg ttgcccctca
     3061 gcgcccggcc ggggccggct cggccgctat gtgctgcgcc gcggcggcag cccggtgtgc
     3121 tgggtcagaa tgcggcctgg cgcaggctgg gtgcgcgctg gcagctcttg tttctgtagc
     3181 tttcgggggc tggcggcggg gctggagttc ttgcgccgcg gggactggta ggcggggctg
     3241 ccggccggtt gccaggccgg gatgaggtgg tgcttgccgc tgccgatcag gtcgtggcgg
     3301 cccatgtcct tgagtgcctg gcgcagcagg ggccagttgg ccgggtcgtg ccagcgcaga
     3361 aaggccttgt gcaggcggcg ccggcgttcg cccttgacgg tgtcgacccg ctcgccgcga
     3421 ccgtcgcggt gcacgccctt gagcgggttg acgccggtgt ggtacatggt ggtggcggtg
     3481 gccatggggc tggggtagaa ggcctgcacc tggtcggcgc gaaagccgtt gcgcttgagc
     3541 cagagcgcga gctggagcat gtcctgatcg ctggtgccgg ggtgggcggc tatgaagtac
     3601 gggatcaggt actgctgctt gccggcttcg gcgctgaagc ggtcgaacag ctggcgaaag
     3661 cgctcgtagt cgccgatgcc gggcttcatc attttcgaca gcgggccttg ctcggtgtgt
     3721 tcgggcgcga tcttgaggta gccgccgacg tggtgttgca ccagttcctt gacgtaggcg
     3781 ggcgagcgca ccgccaggtc gtagcgcagg ccggagccga tcaacacctt cttgacgccg
     3841 ggcagctggc gcacgcggcg gtagacgcgg atcagcgggt cgtggtcggt gcgcaggttg
     3901 gggcagatgc cggggtagac gcagctgggt ttgcggcaca ccgcttcgat ggccgggtcg
     3961 cggcagccca ggcggtacat gttggcggtg gggccgccga ggtcgctgac gacgccggtg
     4021 aagccctgca ccttgtcgcg catggcttcg acttcgcgca cgatggagtc ttcgctgcgg
     4081 ctctggatga tgcggccttc gtgctcggtg atggagcaga aggtgcagcc gccgaagcag
     4141 ccgcgcatga tgttgacgga aaaacggatc atttcccacg ccgggatgcg ggtggcgccg
     4201 tcgtggcggc cgcgttcgtc ggcgtagcgc gggtgggggt tgcgcgcgta gggcagatcg
     4261 aagatgtggt ccatttcggc gctggtgagc ggcaccggcg gcgggttgat ccagacgtcg
     4321 cgcgccgaac ggccctcgcc atgcgcctgc accagggcgc gcgcgttgcc ggggttggtt
     4381 tccaggtgca gcacgcggtt ggcgtgggcg tacagcacgg ggtcgcgccg cacctgctcg
     4441 tagctgggca ggcggatcac gctgcgggcg tggtcggggc ggatcagggc gatcggcttt
     4501 tcaaggcctt tttgggctgc agcgcttgtc ggatcagcgc cagcagcttc tgtttttgta
     4561 gcgtcgcaat ggctgccctg cgcggcggcg cgttcggcgg tggtctggta ggggctgctc
     4621 agggcgtcca cccggccggg gcgatcgacc tcggtggagt cgatttcgcg ccagcccggc
     4681 gccgccgtgc ggcgcacgaa ggcggtgccg cgcacgtcgg tgatctggcc caccggttcg
     4741 cgccgcgcca ggcggtgggc gatctcgacc agcgcgcgct cggcgttgcc gtacagcagc
     4801 aggtcgcatt tgctgtccac cacgatggag cggcgcacgg tgtcgctcca gtagtcgtag
     4861 tgcgcgatgc ggcgcaggct gccttcgatg ccgcccagca ggatgggcac gtccttgtag
     4921 gcctcgcggc agcgctggct gtagacgatg gcggcgcggt cgggccgctt gccggccacg
     4981 tcgccggggg tataggcgtc gtcgctgcgg atcttgcggt cggcggtgta gcggttgatg
     5041 agcgagtcca tgttgcccgc cgtcacgccc cagaacaggt tgggccgccc cagggccttg
     5101 aacggctcgc tgctgtgcca gtcgggctgg gcgatgatgc cgacgcgaaa gccctgcgct
     5161 tccagcacgc ggccgatcac ggccatgccg aagctggggt ggtcgacgta ggcgtcgccg
     5221 gtgacgatga cgacgtcgca cgaatcccag cccagcgcct ccatctcggc gcggctcatg
     5281 ggcaggtagg gcgcggggcc gaagcgcttg gcccagaagg gtttgtagcc ggtcagcgca
     5341 ggcgcgcgca gcggcagggt ggcgacggtg ctcatggcgc ggctggcggg ttggcggcca
     5401 gggtgcggcg gctcggcgtc atgccgccag cgcccttcag gcgattgcag aaacggtagc
     5461 gcctcgcggt cgattgcacc ggcttgcgcc ccatgggttg gcccaagcgt ccgaggcgtc
     5521 gggaggccgg cccattttac cgaccccggc cgctggtcgc tcgccggcgc cggcgggcgc
     5581 tcacttccgc tcgatcagct ggccgcgcag ctcgccttcg gggtaggcgg cggtgcgcag
     5641 attgacgtac cactggcccg acagcaggtc ggcgcgctgg cgtggcgtga gcatggcgcg
     5701 gccttccagt ggactgacca cggtgcggcc cagcgtcagc accgggtcgg ccacctcgcc
     5761 ggccatgccg gggctgtgaa agtgcgccgc gcgcaccggg ccgctgaggc cgctgaagct
     5821 cagcttccac tgcagcaggc cggtgttgcg gttgagcacc gccaccagtt ccccctgccc
     5881 ggtgctggcg ctgggtggca cggcctggcg gccgctcagc tgggcgctga aggcggccag
     5941 ttcgggcagg cgcggcggcg cgctgggcgt ggcccgcggc cggggcgcgg gcggcgggct
     6001 ggcgcagccg gccagcacgg ccagcagcgc cgccgccacg gccaggcggg tggcggcggg
     6061 gatcgacgcg acgtgcgcgc gcgcgggctg gggcaagcgg aacatcaggc gcaccgtagc
     6121 atccattggg cggcacgtgg tgggcattca cccatggata cctcggtgtc ggcagagata
     6181 cgtcctgcac gcgcccggct cagcacacac acaccgcatc agggcaccac ccgcacctgc
     6241 ccgcgcagct cgcccagcgg ataggcggcg gtgaagacgc tgacgtacca gcgccccgcc
     6301 agcaggtcca tggcctgcgc ggcgctgagg ctggcgcggc cttcatagga cggcccgaaa
     6361 ggcgccggcc acaccagcac ggccggcgcg ttctgccccg catcggccgg gccgtgaaac
     6421 tgcacgccgg tgatggcgcc gctcaggccc gaccagcggg tcttccagcg cagcaggcgg
     6481 gtgtcggggt cgtagatggc gtcccactga ccgctgccgg cgctgcgcgt gggtggcatg
     6541 acgctggcgc cgtccagccg cgtgctcagc gtcaccaggc gggcgctggc gtcggtcggc
     6601 ggcaggggcg cgctctgccc ggccggcggc agcggctgcg gcactggctg ggcgggcggt
     6661 accggctgcg tctgcacccc gccgggctgg gccggcggca cctgcgccgg cggcaatggc
     6721 tgcggcatgc ggatcggggg cgggtcgtag cgccggccca gatcgaccga ggcgcaggca
     6781 ctgagcaggc cggcggccac cagccccgcc agcacggcgt gcgaacggtt ccagtgcggc
     6841 aaagacatcg gcagactcct ccagaacgac aacgacaggt ggcggccggc gcgcggccgg
     6901 tgggcgtcga ttgtaggcag ccgcgccggg cgggcatgcc ggctggcgcg ccgcaggccc
     6961 gctgcgcccc tgctatgctg gcccgcccgc catcgcccca ggccgcgcca tgcccacact
     7021 cgatctgcct gcgctgcaac agctcatcca acccctgctg cccggcctgc tgggtgtgcg
     7081 cctgaccgaa gccaccgccg agcgcgtgcg cgccgaaatg acggtgcgcc ccgagctgtg
     7141 caccaccggc catgtcctgc acggcggctc gcacatggcg ctggccgaca ccctgggggc
     7201 gatcggcacg gtgctcaacc tgcgacccgg ccagcgcacc gtgaccaccg attccagcac
     7261 caagttcatc gccggcgcgg ccgaaggctc ggtggtcacc gccgaagccg tcgccctgca
     7321 ccgcggccgc agcacccagg tgtggcagac caccatccgc aacgccgaag gcaggctgtg
     7381 cgcggtggtc acgcagaccc agctggtgct ggcgacccca ggcgagtgat tgcaggccga
     7441 gcgccgccat gtcccagccc ctgagccgcc accggctgca cccacacccg agcacgccgg
     7501 caccggccgt gcgcagcctg tgcgtggacg tcggccgcag cgacacggcg cccggcgcgt
     7561 ggtggctgcg ctttcggctg caggcggcgc tggaggcgct ggcgctgccg ccgcccggcg
     7621 cgtctgccgg gccggcggac gacctgtggc gccacacctg cttcgaggcc ttcgtcggcc
     7681 gcgcgcaggc agcggcctac cacgaattca acttcagccc tgcgggcgac tgggcggcct
     7741 acgccttcag cggcccgcgc cagcgcgcca gcgccgacca gctgccactg gcgccgcgcc
     7801 tgcagtggca gcgcgccgac gacaccctga ccctgaccgt cgagctgccg cgccaggctc
     7861 tgccggccgc cgaccacacc gacctcctgc tgggcctgag cgccgtgatc gaagcgcgtg
     7921 acggccagct cagctgctgg gcgctggccc acccggggcc gcagccggac tttcaccacc
     7981 gcgccggctg gacggcgcgc ctgccctgat ctgcctcaca tgaaattcgg actcgaacgc
     8041 tttcttgaag agcccttgct gcgcgcgcca ctggcgggcc ggcgcgtggc gctgctggcg
     8101 cacccggcct cggtcacgcg caacctgacg cacgcgctcg acgccctggc cgccctgccc
     8161 gatgtgcgcc tgacggcggc cttcggcccg cagcacggcc tgcgcggcga caagcaggac
     8221 aacatggtcg agtcgagcga ctacaccgac ccgcgcctgg gcatcccggt gttcagcctg
     8281 tacggcgagg tgcgccgccc caccccggcc atgctggaga cgtttgacgt gctgctggtc
     8341 gatctgcagg acctgggctg ccgcatctac accttcatca ccaccctgcg ctacgtgctg
     8401 gaagccgcgg ccgcgcacgg caaggcggtg tgggtgctgg accgccccaa cccggtcggc
     8461 cgcccggtcg aggggctgac gctgcgcgcc ggctgggaaa gcttcgtcgg cgccggcccg
     8521 ctgccgatgc gccacggcct gacgctgggc gagctgggcc actggttcat cgcgcagctc
     8581 gggctgcagg tcgaataccg cgtcatcgcc atggaaggct ggcggcccga cgccgcgccc
     8641 ggccacggct ggccgctcga acgcacctgg gtcaacccca gccccaacgc gcccaacctg
     8701 tggatggcgc gcgcctacgc cggcaccgtg atgctggaag gcaccacgct gagcgaaggc
     8761 cgcggcacca cgcgcccgct ggagctgttt ggtgcgcccg acatcgacac cgcgcgcctg
     8821 ctggccgaca tgcgcgcact ggcgcccgac tggctgcgcg gctgccggct gcgcgaatgc
     8881 ggcttcgaga cgacctttca caagcacgcc gggcaactgt gcgccggcgt gcagatccac
     8941 accgaggacg cgacgcacta cgaccacacc accttccagc cctggcgcct gcaggcgctg
     9001 gccttcaaag cgctgcggcg gcaggcgccg gactacccgc tgtggcgcga ctttccttac
     9061 gagtatgaaa cccggctggc catcgacgtc atcaacggcg ggccgctgct gcgccagtgg
     9121 gtggacgacg cggcggccac gccggccgag ctcgacgcgc tgacgcgcgc cgacgagcag
     9181 gcctggcggg cggcccgcac gccattcatg atttatgaat gaaacacggc tccagcgctt
     9241 atccagcaag cgccaaaagc tatcattact gtagtcagcc cagccctatc ggagcacccg
     9301 catggccacc gtcacgatct accacaaccc ccattgcagc acctcgcgca aggcgctgga
     9361 gctgctgcgc agccgcggca tcgagcccac catcgtccag tacctcaaga cgccgcccag
     9421 cgccgccgag ttgcaggcca tcgcccgcgc cagcggcgcg ccgctgcgcg ccctggtgcg
     9481 caccaagcag cccgagtacc tggcgcaggg cctggacgat gcaacgctca ccgacgccca
     9541 actggccgcc gccctgcacg ccacgccggt gctgatcgag cggcccatcg tggtcacggc
     9601 caggggcgcg cggctggggc ggccggtcga gaacattctg gacctgctgg actgatgagg
     9661 gctcgcattc acgatgatcc atgtcccatg acctcgctgc gcgtcgatga tggcgcgagg
     9721 gacgtgaatc cttgctccga accggaaccg gtatgagcgc cacccccccc gctgccgccg
     9781 gtgacggcac cctcggcgcg ccacggtcgc gcgccgacct gttctggtcc ttcagctggc
     9841 tggcactgca gggctttggc ggcgtgctgg cggtggtgca gcgcgagctg gtggagcgca
     9901 agcgctggct cacgcgcgag cagttcgtcg aagactgggc ggtggcgcag atcatgccgg
     9961 gccccaacgt ggtcaacctg tcgctgatga tcggtgaccg ccatttcggc ctgtcgggcg
    10021 cgctggcggc gctggccggc atgctgaccc tgcccacgct ggcggtcgtg ctgctggctg
    10081 cgctctacag caacgtcgcc gacgtcccgc tggtgcaggg cgcactgcgc ggcatgggcg
    10141 cggtggcggc cgggctgatt ctgggcaccg gcatcaagct gatcggtgcg ctgcgcgcca
    10201 accccctggg catgccggcc tgcatcggcc tggcgctggc gaccttcgtc gccatcggcc
    10261 tgctgcggct gccgctgggg cccgtgctgc tggtgctggg cggcgcggcc tgcgtctggg
    10321 cctggaggcg gctgccatga acccggccga cgccctgagc gcggccgact ggctgacgct
    10381 gtggctgcat tttctgtcgc tgtcactgct ggcggtgggc ggcgccatca ccaccgcgcc
    10441 cgacatgcac cgctacctgg tgggctcgca gcactggctg agcgatgcgc agttcaacgc
    10501 ctcgatcgcc atcgcccagg ccgcgcccgg gcccaacgtg ctgttcgtcg ccctggtggg
    10561 ctggcacgtg gggctgaacg ccggtggcgg ccccgccggc ggctggcacg cgcaggggct
    10621 ggccctgctg ggtgcggcgg tggccatgct gggcattctg ctgccctcgg gcgtgctgac
    10681 ctacagcgcc acccgctggg cgcaccagcg gcgcgagctg cgcgccgtgc gcgcgttcaa
    10741 ggccggcctg gcgcccatcg tgatcgccct gctgatggcc accggctggc tgctgagcac
    10801 cgcccacggc cagccggcgc gcgactggcc actgtggctg ctgaccgccg ccaccaccgt
    10861 gctggtgtgg cgcacccggc tgcacctgct gtggctgatc ggcgccggcg cgctgctggg
    10921 aatgctgggc tggatctgaa gggcccagcc acgggtacgc cgcgctcacc atccccggcc
    10981 tgacgcgccc acgacccacg cgccacgacc catcgaagcg ccgcgaggtc atccgtcatc
    11041 ctggaaacgg cagttggacg cgcccgcccg tgccgtgatc gccgcgccat gcggcgagcg
    11101 cggtgcgggc gggggcgtag cgtgttcacc cgggaggtga agtccatcgc agccgccaga
    11161 ccccgcatcg atgcgacctg cccaaagttg acaagcggtc aactgtcgtt tttagggtct
    11221 ttcgtcatcc gcgtcgactg atccagccaa acacccagcc cctgcgcgtt caactcgatg
    11281 tcgaccgcca gcagctcggc caggcgctcc cggccaccgc gccaaccgtg ctgcgcggcg
    11341 cggcgggcgt acagctccag cagcccggcg cgcaggctcc ggtggcgctc gtccgacggc
    11401 tgcggcgcgg cggcgtgggc cagctcgacc atggcgtcga gctgcgcgtg cagatcctgc
    11461 gccagacggg cgggcggcgc caccggtccg tagtgggtca ggcagatggc cggcggctgc
    11521 agcgccagca ggcggtcgat cgagcggtgc agtgccggcg gatcgagctc caccggcgag
    11581 gtggtgggca gaatgaaggc gccctgctcg gtgtcgaatt cgcggtagga cacgccaaag
    11641 gtgtcgccgc tgaacacgca gcccgaggcc aggtcgtgga tggcgttgtg gtggcgcgcg
    11701 tggccgggcg tgtccaggca caccagcggg cgcccggcca gctcgaccac atggccgtca
    11761 ggcgccgcca ccacgcgctc ggccggcacc ggcagcaggc ggccgtagct gcgctgcatc
    11821 tcggcctcgc catacacggc cgccgcgccc gcccacaggc ggctggggtc gatcatgtgg
    11881 cgcgcgccgc gcgggtgcac caccaggcgc gcctgcggca actggcgcag cagctcgccg
    11941 gcgccgccgg cgtggtccag atgcacgtgg gtcaggatca gccagtccac gtcgcccggc
    12001 gtcaacccgg cctgcgcgag cgccgccagc aggtgtggca ccgaatggtt gacgccgcag
    12061 tcgatgaagg caccgcgccc gcgctcgacg atcagatagg ccgcgtccag gtgcgggcgc
    12121 acgaagccgg tgtcgatgac gtggataccg tggtcgaggt tcatgcccgc cactgtacgg
    12181 ccatcgcgac cgctcgatcc gaccaccggc gattccgtcc gcctggcgcc gcgcatggag
    12241 gcccggccgg gccccgggct tgggagacct accaacatgt gaactgcctt gcaaggcagc
    12301 catgacctga ggcgcgcacg tcagcaagcg aagattcctc cgttgcgcgt gccgtcacgc
    12361 cgtaccggcc tgaaccgccc atgacccagg gaaacgcccg gcgcaagcgc aacccaccca
    12421 acgcaagtga ctcaacgcgc ggccagcgcc accctgcgac agtcggcaaa ggcatcgagc
    12481 gcccttgtgt acgtgggcga ctgcacatcc agcaggccca gcatggtgtg gtagaggttg
    12541 tcgtgcgtga gcggcgcatt ggcgccggcg cgcaggcagg cgtccgacaa gcgctcacgg
    12601 cgcagcaggg cgtcatcgag ccaggccacc atgggcacat gcttttgcac gtcgggcgcc
    12661 atgttgtagg gcatgccgtg caggaacaga ccgtattcgc ccagcgactc gccgtggtcg
    12721 ctcaggtaca agaggcccgt gtcgtagcgc gccgagcgtg cctgcagcca gtctatggtc
    12781 ttgcccagga agaggtccgt tgccgcgatg gagttgtcgt aggcgttgac cagctcctgc
    12841 tgggcgcaat cggccagcac ctcggtgcgg cattcgggca aaaagcgctt ggcggcgccg
    12901 tgcgagcgct tgtggtaggc cggcccatgg ctgcccatct ggtgcagcac cagcagcacg
    12961 ccgcgctcgc gctgcgccgc gggcaaggcg gccaggcgcg cgtccaggtc gtgcagcagg
    13021 gcgtcgtccc cgcattcgcc accggggcac agggcggcct gagcatcgcg cggcagagcc
    13081 tcggacgcgc tggcatgcgc cacgcgatcg cacaccccct tgcagccgga ctggttgtcc
    13141 agccacagca cggccatgcc ggcggtctgg gccacgtcca gcaggttctc gtattcgttg
    13201 tcgcgcgact cgaacgcgtc cttgcccagc ggcgagaaca tgcagggcac ggaggccagc
    13261 gtgctcgtgc cgcaggagcg cacctcgcgc cagctcagca cgtcgcggcg cgcgagctcg
    13321 ggcgtggtgt cgcggccata gccgttgatg ccgaaatggt ccgcgcgcgc cgtctctccc
    13381 accaccagca cgaacagcat gggcctggcg cccgccgcat aggatgtccc cagcgcggcc
    13441 ccctgggtca tgggcaccag ggtgcggctg cgcgcaaaca ccggccgcag ggccgccacg
    13501 ggcgtcgaat acaggctggc cagcgggttc atcagatagc gcagctgcgg gtggctgcgc
    13561 atcagcggcg ccaggtcgcg tgcggtggcg gccaccgtgc ctgcggccac ggccagggcc
    13621 agcaccagca tcacgaggtt gcgccacagc tgggccaacg gccccatggc cacaatgcgc
    13681 acgcgcagca gccaccagct gggcagcacg agcacggcca gcacggtcag cagcatgcgc
    13741 aggctcagca ggtcggccgc ctcgcgggcg tcggtctgca gcacattggc cgccatgccc
    13801 ggatccatga ccacctggta ggtcagcatg tagtactgcg ccagcgcagc gaccagcacc
    13861 accagccacc acagcagccg catgccgcgc gcccaggcaa aaaaggcaaa aatggccacc
    13921 atgccgcaga cgatgagcac cgccagcgcc gccatggagc gcaggtactg gctgggcgcg
    13981 ccgccaatgc gcatgatctg cagccacagc ggccagttgg ccgcaagcac cagatagagc
    14041 gacaggcgca gcaccagctg ctgggccgtc aagggcgtgg ccatccaggc gtccacgcgg
    14101 cgcagcaagg cccgcagggg ctggagcaaa ttgaaagagg tgggagagga tggggagccg
    14161 gtgttggcag aggcaagact gagcatgggc ccatcgtgct gtcgtgctct gaaggctggc
    14221 tgaacgaggc gctcaggttt cgttcaggtt tgtatcccgc gcacgggttc tgctgcgcca
    14281 gcgctgcacg gcctcgtcca ccagcaggcc caaggcccag cagaaccatg ccgtccagag
    14341 cgtgtgactc atgtagtgcg cgccgcgcac ctgctgcgac aggcccagcg cgcagcccag
    14401 gacaaggctc gccaacaacc agccggttgc caggcgcggt acggcgcggc gcagcacgaa
    14461 ccagcccgcc acgtagccga acccggccga tgcatggccc gccggaaagc aatggcccgg
    14521 gccaccatcg cgcacgcccc agatccagtg cgacacatag tgcgccacac cgccaaactc
    14581 cgccaggtcc caggggcagc tcgtcgtcgc caggcgcttg agcgccgtga tggacagcat
    14641 ggccagcgcc acgctgaacg cgagttggcg cctgtccgcc gcccccatgg tgcgcagaaa
    14701 cccccacggc cgccatgcgc cccaggccag cgccagcagc gccgccatgc tgagggcgcg
    14761 cggcacctcg tgcagcagca gcacatagct tgggtggctg cgccaggcaa agccctgggc
    14821 cgagccgaac agccgcgcca gcggaagatc cagacccgac gcatcccaca ggagcagcgg
    14881 cacgagcggc agcaagacaa gcgccagtct gcgccataga acatcgaaca tgggcccaag
    14941 cctatgccgc catcctgaac gcgccatgaa cgccccgccc actacacttt ccgcatcgga
    15001 aaaatctacg gagtccccat gcgtgtgctg ctggtcgaag acgatctggc cctgtccgac
    15061 gccgtgtgcg gctatctgcg cgccaaggcc ttcgtggtcg atgccacggc cagcctgagc
    15121 gaggccgagg cggcgctgca tggcgcgcaa tacgcggccg tgctgctcga cctgcatctg
    15181 cccgacggcg acggcctggc cctgctgccc cagttgcgcg cgctgcagca gcgccccatc
    15241 gtcatcgtgc tcaccgcacg cgatcaggtc agcgaccgca tacgcggcct ggacgcgggc
    15301 gcggacgact acctcatcaa gccctacgac cccggcgagc tgcttgcgcg gctgcgcgcg
    15361 gtggagcgcc gccgcagcgc ggctgagact ccggtgctgg tgctgggcga cctgcagatc
    15421 gacctggcgc gcgagcaggt gcgccgcgcc agcgtgccca ttgcgctgac gcagaaggaa
    15481 tgggccctgc tgcgcgtgct ggccacgcgc cccggccgca tccacacgcg cgaaaacctg
    15541 caggacgccc tctacggctg gggcgacgag accgacagca acacgctgga ggtgttcatc
    15601 agccgcctgc gccgcaagct cgggcgcacg cacatcgaga cagtgcgcgg cctgggctat
    15661 aggctgcaca gcgagccggc cgccgcatga gggccagcac gctcgcgcaa cgcctgacca
    15721 gggccctggt gctgcggctg gccctggtgt ggctgctgtg cgtgctggcc gtcgcctatt
    15781 acttgaacca cgagatcgaa gagaacttcg atgccgagct ggccgagagc gcgcaccgca
    15841 tgttcgacgt ggccatgttc cagctccagc gcggcgcgcc ggcgggtgcg gcgggccagg
    15901 cgccgcgtgc tgatgccgtg atcgcagacg atccgctgtt tgccgacgcg cctctcgtct
    15961 accagcttgt cgacgcccag ggccggctgc tgctgcgctc gcgtcaggcg ccggcgcagg
    16021 cgctggccgt gccgctcgag cccggctttc acgacacgaa ccaatggcgc gtgcatgtcg
    16081 cgcagcaccc ggagcaggcg cttttttttc tgctggccga cccattgtcg gagcgcaacc
    16141 acgaatggcg cgagacgctg ctggtgctgc tggtggcggc cgtactcgcg ctggcggcac
    16201 tggcctgggc cctgcccggc gtcgtcacgc gcgagctcgg ggtgctggag cggctgcagc
    16261 ggcaaatccg ccggcgcggc ggcggcgacc tgcggccgct gacgctgacg ggcctgccgg
    16321 ccgaactgcg cgccgtgggc gaggacgtga acctgttgct gcagcgcctg gaccaggcgc
    16381 tggacgtgga gcgcgccctg gccgccaacg ccgcgcacga gctgcgcaca ccgctggcca
    16441 cggcccggct gcggctgcag accgcgctgg agcatggcct gcaccgcgca gacgtgcagg
    16501 ccgcggtcga tgcgctgcgc accctgagcc agcgcaccga aaagctgttg cagctctcgc
    16561 gcgcggaatc cagcgccccg ctctcgcgcg aacccgtcga tctggtgcgg cttgccgcca
    16621 cggtggcgca ggagttctgg caggacgagc gcgcggccga ccggctggag ttgataacgg
    16681 ccaccgatgg ggaacattgc acggccctgg gcgacttcga cgccctggcc atcgccctgc
    16741 gcaacctggt ggagaacgcg ctccactacg cggccccggg cccggtggag atcctcgtcg
    16801 gccccggccc catgctcgcg gtgcgcgacc acggccccgg cgtgagcgcg gaccggctca
    16861 agtccctgca gcagcgccat gtgcgccaga gtgcggaccg cgcgggctac gggctgggcc
    16921 tgtccatcgt ggccaccatc gcagccaagc acgatgccca gctcaccttg acatcacccc
    16981 tgccgcacga gagcggcggc tttgaggcgc gcatgcaatg gacggtggct acaccaccag
    17041 aacgcgcatc cagtcgggca gcggcgccgc cttctgcgcc ggaaagtcca cccagatgac
    17101 cgtggcgcca ccggcggcgg ccagcacgcc aggccggtcc acgcgttcca tggtggtcca
    17161 ggcatcgacc gctatgagac gaagtgacct tggccctgtt tggtgtggtt cctattgatt
    17221 cgccctttcg gggcgccggg ggattccatg gattgccgaa cagaaccggg cacgcgcggg
    17281 catgctgatg acgggtcagt ttcttgcgcc ccacgtcttc cggtgatgcc gccacacggt
    17341 tcgcacagca cctgcggagt tcgcaagcaa caagcccgca gaacgcatcc acatcaccca
    17401 aagcggcacc gtgctggatc acctgagggg attcccgcaa accgatcaag ttcctggcag
    17461 atttgagcaa acggcccaca aattgccgac agggatcagg ataagaaaaa gcccgctatc
    17521 gtttgcatag cgggctttct ttgtctgtgg cggagaggga gggattcgaa ccctcgatac
    17581 ggtaaaccgt ataccggatt tcgagtccgg cgcattcgac cactctgcca cctctcctgc
    17641 ttcgtgtcga aaggcagatt ctagcaggtg ccgcgcaccc gcctcagggc gccaggcgct
    17701 cgatgccgcc cagatacggc tgcagcgcac gcgggatgcc gatggagccg tcggcgttct
    17761 ggtggttttc cagcaccgcc accagcgcgc ggcccaccgc caggccgctg ccgttgaggg
    17821 tgtgcaccag ctcgttcttg ccctgcgcgg tcttgaagcg cgcctgcatg cggcgcgcct
    17881 ggaaggcttc gcagttgctg accgaggaga tttcgcggta ggtgttctgt gccggcaccc
    17941 agacttcgag atcgtaggtc ttgctggcgc cgaagcccat gtcgccggtg cacagcagca
    18001 ccacgcggta gggcagctcc agtttctgca ggatggcctc ggcatgggtg accatctgct
    18061 ccagcgcctc gtagctgcgc tcgggatgca cgatctgcac catctcgacc ttgtcgaact
    18121 ggtgctggcg gatcatgccg cgcgtgtcgc gcccggcgct gccggcttcg gagcggaagc
    18181 acggcgtgtg cgcggtcagt tggaggggca gatcggcttc ggccaggatt tcgccgcgca
    18241 ccagattggt gagcgagacc tcggaggtgg ggatcaggta cagcggctcg ccctcgccct
    18301 cctggccgcc cttcttggcg gcaaacaggt cggcttcgaa tttgggcagc tggccggtgc
    18361 cgcgcaaggt ctcggcattg acgatgtagg gcgtgtagca ctcggtgtag ccgtgttcgc
    18421 cggtctgcac gtccagcatg aactgcgcca gcgcgcggtg cagccgcgcc accgggccgc
    18481 gcatgaaggc aaagcggctg ccggccagct tggcgccggt ctcgaagtcc aggccaagcg
    18541 gctcgcccag ctcgacgtgg tcgcgcaccg cgaagtcgaa gctgcgcggc gccagcccat
    18601 gcggcgccca gcggcgcacc tcgtggttgg cggcttcgtc ggcgcccacg ggcacgctgt
    18661 cgtgcggcag gttgggcacg gccagcagca gcgtctgcag ctcgtcctgg atccgctcca
    18721 gccgggcggc ggaagcgtcg agctcgtcct tgatcgacgc cacctgcgcc atcagatcgt
    18781 cggcgctctc gcccttggcc ttgcgctggc cgatctgctt gctggcgctg ttgcgctgcg
    18841 cctgcagctc ttcggtgcgg gtctggatgg ccttgcgctc ggcttccagc gcctggaagg
    18901 ccgcgacgtc cagaaagggt tggggatttt tgcgggtcag caggcgtgcg acgacgccat
    18961 cgaggtcttt gcgaagcagc agtggatcaa gcatggtccg attgtagagg cgcgcggcgg
    19021 cgccccggcc gcccccgatt caaagcaaaa gcagccgttt gcgcccgcct ggcaagcgcc
    19081 aacagctatc aaaatcatag ttttcagcgc agatgcgcga gaccgtcttc gctggcgctg
    19141 tcgagcttga ggcccttggg caagggaaat ttcatggttt cctcgatgcc ggcgagctgg
    19201 cgcacggaca ccgcccccag cgcgcgcagc cgctcgatca cctggcgcac cagcacgtcc
    19261 ggcgccgagg cgccggccgt caggccgatg cgcgcgcggc cctgcaccca ttcgggcttc
    19321 aggtcgtcgg cgctgtcgac catgtgggcg gcggtgccga gcttgagcgc cacctcgcgc
    19381 aggcggttgc tgttgctgct ggtggggctg ccgaccacga tcaccacgtc cacttcgcgc
    19441 gacagcagct tgaccgcgtc ctgccgattc tgggtggcgt agcagatgtc ctgctgcttg
    19501 ggctcgcgca ccatgggaaa gcgcgccttc acggcggcgg tgatttcggc cgcgtcgtcc
    19561 acgctcagcg tggtctgggt caccaccgcc aggcgctcgg tctgcgtcgg ccggatgcgt
    19621 gccacgtcct gcacctcctc gaccaggtgg atgccgccgg gcaactggcc catggtgcct
    19681 tccacctccg ggtggccctt gtggccgatc atgatgaatt cgtagccctc gcggtgcagc
    19741 ttggcgactt cgacatgcac cttgctgacc agcgggcagg tggcgtcgaa cacgcgaaag
    19801 ccccgcgcct gggcctcgcg ctcgaccgcc ttgggcacgc cgtgcgcgct gaacaccagc
    19861 gtggcgccgg gcggcacctc ggccagctct tcgatgaaga tcgcgccctt ggccttgagg
    19921 tcattgacga cgtaggtgtt gtgcacgatc tcgtggcgca cgtagatggg cgcgccgaac
    19981 ttgaccagcg cgcgttcgac gatttcgatg gcgcggtcca ctccggcgca aaagccgcgc
    20041 ggctcggcca gcaggatgtg ggcgtcgacc ggcggcgccg cggcagcctc gccgtccgca
    20101 gcggcggcct tgcgcgtgat ggtgatggag cggccgtggg actgcggcat cacaggatcc
    20161 cgatcacctg gacctcgaag gtcaccggct ggccggccag cgggtggttg aagtccagcc
    20221 gcagcgcgcc atcgtcgcgc accgcctgca ccacggcgcc gaactgaccc tgaccatcgg
    20281 gcgtgggcaa ctggatcacg tcgcccacgg cgtagcgctc ctgcgggtcg cccagctcgt
    20341 ccagatcctg gcgtgccagc cactggatca tgtccgggct gcgctcgcca aaggcctcgc
    20401 cggcgggcag ctcgaacgtg gcgcgcgcgc cctcggccag gccgatcagg cgctgctcga
    20461 tcgccggcga gagctcgccc gcgcccagcg acagcgtggc cggcggcccg ccgaaggtgt
    20521 tgatcacgtc gccctgcggg cccgacaggc gatagtgcag ggtcagaaag gcgcctggct
    20581 cgacagtgct catggatgtg tcccgataaa ctgccccgat tctagaaaac ccccgccccc
    20641 ggaccccgca tgtcgctcaa agaccttccc gccgacgcgc gcccgcgcga aaagctactg
    20701 gcgcgcggcg ccgctgcgct gagtgacgcc gagctgctgg cgctgctgct gcgcaccggc
    20761 accgcggggc gcggcgtgct gcagatggcg caggagctgc tggaccggtt tggcggcatc
    20821 gccggcctgc tgcacgccag cgccgacgac ctggggcgct tcaagggcct gggcggcagc
    20881 gccaagcggg ccgagctgat cgccgtgctc gagctggcgc gccgcgcgct ggccgagcag
    20941 ctcaaggaaa aaccggctct ggacagcgcc agcgccgtca agcagtacct gcagctgcac
    21001 ctggggcaac gtccccacga ggtgttcgcc gccctgttcc tggacgcgca gcaccgcttg
    21061 gttgcgctgg aagaactgtt tcgcggcacg ctgacgcaga ccagcgtcta cccgcgcgag
    21121 gtggtgctgc gcgccctgca ccaccacgcc gccgccgtgg tgctggcaca caaccacccc
    21181 agcggccagg tcgcacccag ccgcgccgac gaggcgctga cgcagaccct gaaagccgcg
    21241 ctgtccctgg tggacgtgcg cgtgctcgac cacatcatcg tcgcgcccgg gcaggcgctg
    21301 tcgatggcgg agcaggggtt gctgtgaaag ctcgctccct gcaggatctg aagcagctgc
    21361 gcacccagct ggccgagcgc gaggcgcgcg cggcgcaggc gcaggccgag cgcgccgcgg
    21421 cgcagcagcg cgcccgcgcc gaccaggacc tgtttgcccg cgccatcggc gccacgcagc
    21481 cgctgcctgc gcatggccgg gtggtgcacg cgccgcccgc ggtggcgccc gagccgcgtc
    21541 agcgccagcg cgacgagcag gcggtgctgc gcgaatcgat ctccgacgag ttcgatacca
    21601 gcaccctgct gcacaccgac gagcacctga gctttcgccg tcccgggatt ggccccgatg
    21661 tggcggccaa gctgcgccgc ggccactggg cggtgcaggg tgagatcgac ctgcacgggc
    21721 tgcgcaccga cgaggcgcgc gaggcgctgg cgcagttctt gcgcgaggcc cagcgcgccg
    21781 gactacgctg cgtacgcgtg gtgcatggca agggtctggg ctcgcccggc cgcacgccgg
    21841 tgctcaaggc caaggtgcag gtctggctgg tgcaaaaggc cgaagtgctg gccttcgtgc
    21901 aggccaagcc gctggaaggc ggcgcggggg cgctgctggt gctgctggcc gcctccatcc
    21961 gttgacggtt caccgtccca cggggctggc gatcgtcagg cgcgttcagc aggcccgcaa
    22021 gcaggagcaa cgccgcatgg cgcggcgagc gcggtgtggg ttgtcgggat gcacggcaag
    22081 ccgctggacc atgaacggcg ttcactatga tttcaggagc tgctggcgct tgccagacaa
    22141 gcgcaaaagc ctagtgaata cggtttgcca gcaaggtcca gacgggtgtc aggtcagccg
    22201 gcaacggtgg cagttggccg aggtcggtgt ggctctcgtc ggggctgtgg aagtcgctgc
    22261 cgcgcgaggc ggccagaccg aattcgcgtg ccatggcggc gtacctggcg gcttcggcgg
    22321 cggtgtggct gccggtgatg acttccaccg cctgcccgcc atgctgcttg aactccgaga
    22381 acagcgcgta ttcgacggtc ggcgagatgt cgtagcgcgc cgggtgggcg atcaccgcca
    22441 cgccgccgct ctgggtgatc cagcgcacgg cgtcgcccag tcgggcccag cggtgcggca
    22501 cgtagccggg ctttccctcg accaggtagc ggcgaaacac ctcggcggtg tcgcggcaga
    22561 cgccggtttc gaccagaaag cgcgcgaaat gcgtgcgcga gatcaggcgc gggttgccga
    22621 cgtacctgag cgcaccctcg taggcgccgg ctatgcccac gcgggccagc tgctcgccca
    22681 tctcgacggc gcgcgggccg cggccgtcgc gcgtgtcggc caggccttcg gccagtcgtg
    22741 ggtcggtgtg gtcgaagccc agaccgacga tgtgcaccgt ctcgtcggca aaggtgaccg
    22801 acacttccac cccggtcagg tagcgcagcc cctcggcgcg tgccgcctcc agcgcgcgct
    22861 gctggccacc gacttcgtcg tggtcggtca gcgaccacag atccacgccg ttggcgcgcg
    22921 cacgcgcggc cagttgttcg ggcgtcagcg tgccgtcgga aacgacggaa tggcagtgca
    22981 ggtcggcgtt gtgcggaaag ctcacgccgt cgattctacg aaggcgggca agacggcgtc
    23041 gccggcgggg tgttggccag cgcggagctt cagggcgccg gcggcgcggg ccaggggcgc
    23101 tgcggcccgc gcaactgctc gaaggcgcgc gccagttgca gcacgcccag gtcgtcgaag
    23161 cgctggccgg cgatctgcag gccgatcggc agcatggcgc ccggcgtggc gggcgacgcc
    23221 atgtagccgc agttgatcga gatggccggc tgctcgccca tgttgaaggg cacggtgaag
    23281 ccgatgtgct ccagcccgcg cagcgggtcg ttggtgggcg aaggccattc ggccggcgcc
    23341 ggtgcgttgg gcgaaaccgg cgagatcagg tagtccagcc cctgcatggc ctggctggtg
    23401 gccacgcgcg tggcgtgggt ggcggcgtag gcacgaaaca ggtcggcgcc gctcatgccg
    23461 cgcgcgcttt cggcccagtc gcggatcacc ggcagcacgc tggcgcgcgc cggcgcgctc
    23521 agcgcgtcca gatcgagcag cgagcgcatg cgccagaagt ggtccagccc ggccagcagc
    23581 tcgggcgtca tgaagggtgc ccacggcgtg acgatggcgc cctgcccttc gagcagccgg
    23641 gcggcctgtt cgacggcggc gcgaatggcc ggctccaccg gcaggccgca gccggcttgc
    23701 agcagcaggc cgatgcgcag gcccttgagc ggcttgtcct ggcgccaggc cagcgaccag
    23761 tcgatggcct gcggcggcag gctcatgcag tcgcgcgcgt cgggctggct gagcacctgc
    23821 atcagcagcg ccgcgtcctg cacgctgcgc gtcatggggc cggcggcgcg gcccatgtag
    23881 ggcggatcga tcggcacccg gcccaggctg ggcttgaggc cgacgatgcc gcaccaggcg
    23941 gccggcaggc ggatcgaacc gccgatgtcg gtgcccaggt gcagcggccc gtagccggcg
    24001 gcggcaccgg caccggcacc ggcgctggag ccacccggcg tcagcgccag gtcccacgga
    24061 ttgcgcgaca gccggtggaa ggtggacagg cccgaggaca gcatgccgta gtcgggcatg
    24121 gtggtcttgc acaggaggat ggcgccggct tcgcgcaggc gcgcggccgg tggtgcgtcg
    24181 gcggttgccg gcagcagggg cacggcgcgc gtgcccagcg gcaccgggtc gccggcggtg
    24241 gcgatgttgt ccttgatggt gaccggcacg ccgtccagcg cgccgcaggg ctcgccgcgc
    24301 cgccagcgcg cctcggacgc acgcgcctgc gcgcgcaccg cgtcgggccg cagcagccag
    24361 ctggcggcga tgtgcggctc ccagcgttcg acgtggtgca gcacgctgtc cagcacgtcg
    24421 accggcgaga agtggcgcgc ccggtagccc gccagcagcg cgtgtgcgga cagatcgtgc
    24481 aacggttcgg tcattctgaa gttcaagcaa aaagagccgc tggcgcttat ccagtgtgcg
    24541 ccagcagctc catatttaat agcaaacgtt caaccccagc tgagtgacga gaggtcgttg
    24601 acgaagatcg gcgattcctt ccacaggccc tgcacgcgct tgttggccac cgtcacgccc
    24661 tgcggctgga acagccaggc cagcacggcg tcgttggcca gcagcttctg cagatcgccc
    24721 agcagcttgt tgcgctcggc ggcggtgggg gtgttcttga tccgggtgta gagctcgttg
    24781 aacttcgggt tctggtagcc ccagtaatag tcgggcttgg cgaagttgcc cagatcgaac
    24841 ggttcgacgt ggctgatgat ggtcaggtcg taattcttgg ggccgccgaa ggtgttggac
    24901 agccactgcg cccattccac cgactgcacc ttggcggtga tgccgatctt ggccagctgg
    24961 gccacgatca cttcgccacc ctgacgtgcg taggacggcg gcggcagcac cagcgacagc
    25021 tccagcggcg tggtgacgcc ggcttccttg agcagggcct tggccttttc cgggtcgtag
    25081 gggttgatgc cggtggtgtc gacgtagccg ggcgcaccgg gcacgtagtg gctgccgatg
    25141 ggcacgccgt agccgtcggc ggcgccggcg atgacggcct tgcggtcgat cgccatgctg
    25201 atggcacgcc gcacgcgcac gtcgttgagc ggcttgcggg cgttgttgat ggccagcacg
    25261 gtcttggcgc gcgagccggt gatgagggtc tgcaggcgcg ggttggcctt gaactgcgac
    25321 gcgccgcgcg gggccacgcg cgggaaggcg tcgacgtcgc cggccagcac cgccgccacc
    25381 tgggccgccg ggtcgctgat gaagcggaag gtggcgcgct tgatccggat ggcgctggcg
    25441 ttgcgaaagc cgggccaggc cttgagggtg atggacgagc ccttggccca gttgtccagc
    25501 tcatagggac cggtgcccac cggccgggcg ttgttgccgt cggcgctctt gggttcgacg
    25561 atgacggcgg tggcctggcc catgtagaac aggaagtccg ggtcgggctc cttgttgatg
    25621 atcaccacgg tgtgctcgtc gacggcgctg acgctttcca tggcggcgaa ggtgcgcttg
    25681 tccttgttgg tgctcttgtc gccggcggcg cgcgtgaagc tgaagcgcac cgcctgggcg
    25741 ttgaacggct cgccgttgct gaatttcacg cccttgcgca gatagaaggt gatggtgcgc
    25801 aggtcgggcg atacctccca gcgctcggcc agcaggggcg agacgctgcc gtcggggttg
    25861 atcttggtga gcgtctccag gatgttgtag tgcaccacct cggcgatggc cgaggcggcg
    25921 ccggccgtcg ggtccagccc cggcggctcc agcgtcatgc ccagcaccat ggtgctcttg
    25981 ccggcctggg ccagcgccgg cagatgggtg ctggcggcca gcgcgggcag gctgagggcg
    26041 aagtcgcggc gtttcatcgg cagatctcct cgaggtggat gaaatggagg tgcatcgtag
    26101 cgcaggcgcg gcggcgcgcg ctcagaaggc gggcgcgcgt ccagggtgcc caaagggagc
    26161 gccccagcgc cgccgaccaa ggcgccaggc gcggccgtcg caggggcggc ggcgaacctt
    26221 ggccacgccg agcgggcgtg ccggggcgat cacggcgggc acgaggggcg caggcacctt
    26281 ctcagacggc ggcgagcagc gcagacacgg gcacggcgtc gagcagctgg cgtgtgtagg
    26341 ggtggcgcgg ctcgcgcagc acctggcgca ccgggccatg ttcgaccacc cggccgccct
    26401 gcatcaccgc cacgtcctgg cacaggtggg ccaccaccgc caggtcgtgg ctgatgaaca
    26461 ggaaggtgac gccgtgcgcg tcgcgcaggt cggccatcag gttgagcacc tgcgcctgca
    26521 ccgagacgtc gagcgcgctc accgcttcgt cggccacgat cagcttgggg cgggtgatca
    26581 gggcgcgcgc gatggcgatg cgctggcgct ggccaccgga aaactcgtgc gggtattggt
    26641 cgagcgcatc ggcgtgcagg ccgacggcgg tgagcatggc ggcggcgcgc tcgcgctgct
    26701 cggcgcggtc ggcgccttcg agcgcggcgg ccggctcggc cacggtgcgc cagatgcgcc
    26761 ggcgcgggtc gagcgagccg taggggtcct gaaacaccat ctggaagtcg cgccgcgccg
    26821 cgcgcagctc atcgggcgac agagcgtgca ggtcgcgccc ttgcagcagc acctggccgg
    26881 cggtggggcg ctcgagtgcc atcaccaggc gcgccagcgt gctcttgccg gaacccgatt
    26941 cgccgacgat gcccaggctg cgcccggccg tcacctgcag cgacacgccg cgcagcgcat
    27001 gcacctggcc cggcgttctg aacagcgact cgcgcggcag cgtgtagcgc cgctcgagct
    27061 cacgggtttc aagcaaaaag tggttgtccc gcttatccat caagcgccac cagctattga
    27121 ttcaggagca tgaagaacag tgtcggcacg ccggcaccag aagcggtgcg acgccgtcag
    27181 cgccaccggc ggggccgggt cggtcaggca cgccggctcg gtgtaggcac agcggccggc
    27241 gaacgggcag ccgggcggca ggccgaacag atcgggcacg ctgccggcaa tggtgggcag
    27301 gcggtcgctg cggtgcgcat gcaggcgctg cgccaggttg ggccgggccg ccagcaggcc
    27361 gcgcgtgtag ggatgggccg gctgccggaa cagctcggcg gccggcccgc tttcgaccac
    27421 cgcaccgcca tacatgacca gcatgcggcg cacgttctgc gcgatcaggc cgaggtcgtg
    27481 cgagatcagc accagcgcca tgccgcgttc ggccaccagc tcgcggatca gtgccaggat
    27541 ctgctgctgc acggtgacgt cgagcgcggt ggtcggttcg tcggcgatca gcacgtcggg
    27601 ctcgcaggcc agcgccatgg cgatcatgat gcgctggcgc tggccaccgg agaactggtg
    27661 cgggaagctg tcgatgcgtc gggccggctc cgggatgccc acgcgtgcca tcagttcgat
    27721 ggcgcgctgg cgcgccgcct gggccgacag gccgcggtgc agccgcagcg gctcggcgat
    27781 ctgggcgccc acgcggtgca ccgggttgag cgccgtcatg ggctcctgaa agatcatggc
    27841 gatgcggttg ccacgcaggc ggcgccagtc gcggtcgggc aggcccagca gctcctggcc
    27901 gtccaggcgc aggctgccct gcacggtggc gttgtcgggc tgcaggccca tcagggccag
    27961 tgcggtcatc gacttgccgc agcccgattc gccgatcagg cccagggtgt cgccacgctc
    28021 cagcgcaaag ctgacgtcgc gcaccgcggt ggcgctgccg cggtgggtgc gcaggctgac
    28081 gcgcaggtgt tcggcttgca gcagggccac ggcttcagct cgacagcagg ttgggcaggg
    28141 ggcgaccgcg gctgtcgcgg cgcacgatgc aagcggcgtc gatgcccagg atgcggcgca
    28201 gcccgcggcg cgcggcggct tcggccctcg acgcaccagc cggattgagc ggcattctgg
    28261 caaagcgccg ggcgatgcgg cccgcccgct tgtgcgccag cacctcgtcc ccgtgggcgt
    28321 ggcgggcgct ggccagatcg gcggtcagcg cgtcgcgcca cagctcgctc agactgcgcc
    28381 cctcggccgc actttgctgg cccagtgact gctcaaggta gggcgggctt ttgacggtga
    28441 gggtcatggc tggactgtag cgacttggta ttaccacttg catgaccgat tcagcgcgac
    28501 ccttgcaggc gcggatccag cacgtcgcgc agcccgtcgc ccagaaggtt cagccccagc
    28561 accgacagcg cgatcgccag ccccggccat accgccagca gcggggcgtc gaacatcagg
    28621 ctctgcgcct cggccagcat gcggccccag ctgggctgcg gcggctgggt gcccagaccc
    28681 aggtaggaca gcgccgcctc ggccaggatg gcgacggcaa agcggatggt ggcctgcacg
    28741 atcagcaccg ccagaatgtt gggcagcaca tgctgccagg tgatggccca ggcgcccttg
    28801 ccgctggcgc gcgcggcctg cacgtagtcg cggctccaga cggcgttggc ggcggcgcgg
    28861 gtgatgcgcg cgaaggcggg gatgccgtag atgccgatgg caatgatggc gttgacgatg
    28921 cccggcccgt acaccgccgt cagcatgatg gccgacagga tggccgggaa ggcgaaaccg
    28981 aaatccgcca cccgcatgat cagctcttcc acccaaccgc gccgcgccga cgccagcagg
    29041 cccagcgcag ccccgaacac gaggccgatg ccgaccgcga tcacgcccac cagaatggag
    29101 gcgcgcgcgc ccaccagcag cagcgacacc acatctcgcc cgaaggcgtc ggtgcccagc
    29161 cagtggcgcg cgtcgggccc cagcagcttg tcgtccatct gcacctcgta gacggagtac
    29221 ggcgtccaca catacgacag cgcagccgac agcagcagcg ccagcgtcag cagcgcgccg
    29281 atgacaaagc cagggtggcg ccgcgcgcgc gcccagaagc caggggcggc gccgacggcg
    29341 gcggcaagcc gaagggtgcc gctcatgacc gcgatcgctc cacgccactg cggtcgatcg
    29401 agccgacacc catgcgcatg gggcgcaatg gtttcgccgg gcaaaccagg aatcgccctg
    29461 gcaagttccg cctgccctgc cccatccggg cgtccatggc gcacgaaggt tgggtggtga
    29521 atggccgact catacctcgc tcgctttcac ccgcggatcg atcacggcat acagcacatc
    29581 gacgatgaag ttgaccacca ccaccatggc ggccagcagc atcacgcagt tgcgcaccac
    29641 gatcaggtcg cggttggcga tgctctggaa gatcagccgc cccaggccgg gcaggtagaa
    29701 cacgctctcg accacgatgg tgccggccag cagctcggcc gactgcaggc ccatcacggt
    29761 gatcaccggg atcatggcgt tgcgcagcac atggccccac agcgtggccc ggcggctcag
    29821 acccttggcg cgcgcggtgc gcacgaagtc ctcgcgcagc acctccagca ccgccgaacg
    29881 ggtgaagcgc gccaggatcg ccgcctgcac cagcgccagc gccacggccg gcaggatcag
    29941 cgccttcagg ccctgccaca gcccggccca cacgccgtca tcgaagtacc agccatcgaa
    30001 gccgccggcc gacagccatt gcagcttgac cgaaaacagc aggatcagca ggatcgcgaa
    30061 ccagaaattg ggtatggcga tgcccacctg cgccagcgcc atcaggccgg tgtcgcccag
    30121 acggttgtgc tgcgacgccg cgtacacccc gaccgccagc gccagcagcg ccgtcagggt
    30181 catggccagc accgccagcg gcacggtcag cagcaggcgc tcggcgatca gctcggccac
    30241 cggcgagcca taggcgtagg agttgcccat gtcgccacgc agcaagccgc gcatccagtc
    30301 gacatagcgt gccagcggag gccggtccaa gcccagctcg accaccttgg cggccacggc
    30361 ggccggatcg gcgtcgggcc ccatcagcac ctgggcggcg ttgcccggca gcacctccag
    30421 caccgcgaac acgatcacgc tggtggccgc cagcgtcgcc agcagcgtca gaatgcgttt
    30481 gaagaggaaa tagctcatgc gcggcaaccg gtgcagcgtg gcattatcaa tacatcgggc
    30541 gccgcgtccg gcgcgccctc gaacccgccc acaggagcgc aagcatggcc ctgatgatca
    30601 ccgacgactg catcaactgc gacgtctgcg aacccgaatg ccccaatcag gcgatcgcca
    30661 tgggtgaaga gcactacgag atcgaccccg acaagtgcac cgaatgcgtc ggtcacttcg
    30721 accagccgca gtgtgtgcag gtctgcccgg tggactgcat tcccgtcaac ccacagcgca
    30781 tggaagaccc gcagcagttg ctggacaagt tccgccgtct gcttgggcag gcctgacgga
    30841 tgctcctgat tgaatagctg cccgcgcttg ctgcaggcgc gccagcgtca ttctggacgt
    30901 gcaggtacca ggatgtcgcg cttgccgtcg gccgtcatgg cgctcaccag tccggcgttt
    30961 tccatgtcct cgaccaggcg cgcggcgcgg ttgtaaccga tcttcaggtg gcgctgcacc
    31021 agcgagatgc tggccttgcg gtttttcagc accacctcga ccgcctggtc gtacatcggg
    31081 tccttctcgc cgccggcgtc gccgccttcg ccgaaggcac ccgcatcgcc ctccgccgtg
    31141 ccgccttcga gcacgccttc gatgtagttg ggctcgccct ggctcttgag gtattcgacc
    31201 acgcggtgca cctcgtcatc gctgacgaag gcgccgtgca cgcgcaccgg caggccggtg
    31261 ccgctggcca tgtagagcat gtcgcccatg cccagcagcg cctcggcgcc catctggtcg
    31321 aggatggtgc gactgtcgat cttgctggag acctgaaacg cgatgcgcgt gggaatgttg
    31381 gccttgatca ggccggtgat cacgtccacg ctggggcgct gggtggccag gatcaggtgg
    31441 atgccggcgg cgcgcgcctt ctgcgccagg cgggcgatca gctcctcgat cttcttgccc
    31501 accaccatca tcaggtcggc cagctcgtcg atgatgacca cgatgtaggg caggcgctcg
    31561 agcggctcgg ggtcttcggg cgtcaggctg aaggggttgt agatgaactc gccgcgcgcc
    31621 ttggcgtcgt cgatcttctg gttgaagccg gccaggttgc gcaccccggc cttgctcatc
    31681 agcttgtagc gccgctccat ttcggcgacg caccagttca ggccattggc ggcctgcttc
    31741 atgtcggtga ccaccggcgc cagcaggtgc ggaatgcctt cgtagaccga catttccagc
    31801 atcttggggt cgatca
//
LOCUS       DAOQTZ010000018        31096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1703_length_31096_cov_13.781611, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000018 DAOQTZ010000000
VERSION     DAOQTZ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 31096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31096
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1703_length_31096_cov_13.781611"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PKN51_03765"
     CDS             complement(<1..1419)
                     /locus_tag="PKN51_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNT84247.1"
                     /translation="MGKITGFLEFERIEERSEAVPARLKTWKEFVIGLDGAQARTQAA
                     RCMDCGTPFCNHGCPVNNLIPDFNDLIYHDDWRRAIDVLHQTNNFPEFTGRVCPAPCE
                     AACTLNINDDAVGIKSIEHAIIDRAWAEGWVTPQPAAIKTSKKVAVIGSGPAGLAAAQ
                     QLARAGHAVTVFEKNAQPGGLLRYGIPDFKLDKGHIDRRIAQMSAEGVVFRTGVLVGE
                     LDPDSKITRHGEQTLSAEQLLAEFDAVLLTGGSEVSRDLPVPGRALDGVHFAMEFLPQ
                     QNRVVAGDNLKGQIHAKGKHVIVIGGGDTGSDCVGTSNRQGAASVTQFELMPMPPEQE
                     DKPLTWPYWPYRLRTSSSHEEGCERQFAIATKEFVAGTGKDKGRIVGLKTVEVEWAGG
                     KMTEVPGTEKTWKADLVLLAMGFVHPVGRVLDAFGIDKDARGNARAGTDVVHGYRTSV
                     ERVFAAGDMRRGQSLVVWAIREG"
     gene            complement(1444..6180)
                     /locus_tag="PKN51_03770"
     CDS             complement(1444..6180)
                     /locus_tag="PKN51_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNT84248.1"
                     /translation="MSHPLPAPHEEILAAAAGGLYQPTHEHDACGVGFIAHIKGHKTH
                     DVVTQGLRILHNLDHRGAVGADKLMGDGAGILIQIPDEYYRAEMAAQGVTLPPPGEYG
                     VGMIFLPKEHASRLACEHELERAVRDEGQVLLGWRDVPVDRDMPMSPTVRAKEPVIRQ
                     IFIGRGRDVIVPDALERKLYVIRKTASAAIQRLRLTHSHEYYVPSMSCRTVIYKGLLL
                     ADQVGQYYRDLADPRTVSALALVHQRFSTNTFPEWPLAHPYRFVAHNGEINTVKGNFN
                     WMRAREGVMRSPVLGEDLQKLYPISFEGQSDTATFDNALELLVSAGYPLAQAMMMMIP
                     EAWEQHETMDERRRAFYEYHAAMLEPWDGPAAMVFTDGKQIGATLDRNGLRPARYIVT
                     DDDHVIMASESGVLPIPESRIVSKWRLQPGKMFLIDLEQGRIVNDEELKTLLSNARPY
                     RQWIENVRVRLADTPSDGARPSYTETLLDRQQAFGYTQEDIKFQLLPMAQQGEEAIGS
                     MGNDTPLAVLSDKNKPLYNYFKQLFAQVTNPPIDPIREAIVMSLHSFIGPKPDLLDIN
                     AVNPQMRLEVEQPILDFDDMARLRAIERHTGGKFKTYTIDITYPLSWGREGVEAKLAS
                     LCAEALDAIRGGHNILVISDRRMDRDHIAIPALLALSAIHHHLVHAGLRTTAGLVVET
                     GSAREVHHFAVLAGYGAEAVHPYLAMETLTALHEATPEGPSPKKAIQNYVKAIGKGLS
                     KIMSKMGISTYMSYCGSQIFEAIGLKEDFVAKYFRGTATQVGGIGVFEVAEEALRQHL
                     AAFGDDPTLQNMLDAGGDLAWRTRGEEHMWTPDAIAKLQHSVRGGKWDTYKEYAQIIN
                     DQSRRHMTLRGLFEFKVDPARAIPLDQVEPAAEIVKRFATGAMSLGSISTEAHTTLAL
                     AMNRIGGKSNTGEGGEDPARYRNELKGIPIAAGTRVSEVIGSRVIESDYAMQAGDSLR
                     SRIKQVASGRFGVTAEYLVSADQIQIKMAQGAKPGEGGQLPGGKVSEYIGFLRYSVPG
                     VGLISPPPHHDIYSIEDLAQLIHDLKNVNPQASISVKLVSEVGVGTIAAGVAKCKADH
                     VVIAGHDGGTGASPWSSIRHAGTPWELGLAETQQTLVLNRLRSRIRVQADGQMKTGRD
                     VVIGALLGADEFGFATAPLVAEGCIMMRKCHLNTCPVGVATQDPVLRRRFSGKPEHVI
                     NYFFFVAEEVRTIMAQLGLRRFDELIGRADLLDTRRAITHWKAQGLDFSRVFHQPAVP
                     SEVPRLQTETQDHGLARALDHKFIERCQPALERGERVQLMDEVRNVHRSVGAMLSGEL
                     VRRHPEGLPDHTVVMQLEGTGGQSFGAFLARGVTLHLIGDANDYTGKGLSGGRIVVRP
                     SIDFRGDATRNIIVGNTALYGATSGEAFFRGVAGERFAVRLSGATAVVEGTGDHGCEY
                     MTGGTVVVLGSTGRNFAAGMSGGVAYVFDEDGRFSERCNTSMVALEKVPPRDAQEGIA
                     AGWHRAMADEDLLRKLIEEHLRWTGSLRAREILDHWADSRARFVKVMPREYQRVLSAH
                     AEQAAIATADSALVKAKASAALASGQ"
     gene            complement(6447..7118)
                     /locus_tag="PKN51_03775"
     CDS             complement(6447..7118)
                     /locus_tag="PKN51_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT84249.1"
                     /translation="MARLPRLVLPGQPHLVLQRTLDHRPAFVDDEDRAQFMVALRTTL
                     ETEAVQLHAFALRDHELLFLVTPAQPDALARLMQAIGRRYVTAYNRRHARSGPLWDGR
                     FRTSAVEPGEHTLTAMTWIEQATPTPGITSAPHHAGTQPNPLITDPPEYWQLGNTPFE
                     RESAWRERLARGVAGTTEARLRRLVLGGWALGSPEFIRSADATGRPAAPRRPGRPKRA
                     ASNPR"
     gene            complement(7243..8706)
                     /gene="ntrC"
                     /locus_tag="PKN51_03780"
     CDS             complement(7243..8706)
                     /gene="ntrC"
                     /locus_tag="PKN51_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNT84250.1"
                     /translation="MKPIWVVDDDQSIRFVLERALSREHLPVRSFSRPQDVLNEFDAG
                     VEPQVLVTDIRMPGSSGIELLTEAKRRHPQLPVIIMTAYSDLDSAVGAFRSGAFEYLP
                     KPFDVNRAVELIRRAAEDHSLRDQPHADDSDQQPLMLGEAPAMQEVFRAIGRLSQSES
                     TVLITGASGTGKELVALALHRHSPRARGPFVAINTAAIPKDLLESELFGHERGAFTGA
                     QAMRRGRFEQAAGGTLFLDEIGDMPLELQTRLLRVLSDGQFYRVGGHQPLRADTRVIA
                     ATHQDLEERVRQGTFREDLFHRLNVIRLRLPPLNERREDIPLLTRHFLARSARELGVE
                     SKHISPAALERLTQFDFPGNVRQLENLCRWLTVMAPGQRIEAEDLPADLLPRGEPRPL
                     TGEEKATAAITPPHPDAPPSAAAPADWTVALAQEARTRLAAGESGVWDSLAGRFEAAL
                     LHTALGLTHGRRAEAAQKLGIGRNTITRKIQELGLDD"
     gene            complement(8703..9767)
                     /gene="glnL"
                     /locus_tag="PKN51_03785"
     CDS             complement(8703..9767)
                     /gene="glnL"
                     /locus_tag="PKN51_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNT84251.1"
                     /translation="MVALVRVDGTCLLVNAALEHAVRQSRRKLVQARALDWFVDPAPL
                     QAALHSTASHHLAVSRFEGALLRQPPGAGELPVHVVVTPVGRGGPEGAVMVEMIEIER
                     QVQLDREERAQDLIQAHQELLRNLAHEIKNPLGGIRGAAQLLAMEFTSPEYGEYTEVI
                     IREADRLQALVDRLLAPHRGMPQWSDFNIHEVCERVRALVLAEFPRGLQVQRDYDTSL
                     PELRGDRERLIQALLNIVRNAAQALAARIVTGDARIVLRTRIARQVTLNGRRFRLAIQ
                     LQVEDNGPGIPPALRERIFQPLVSGHAAGSGLGLTLAQGFIQQHGGTIECESQPGATR
                     FTLLIPLPDPTACRDIEGPP"
     gene            complement(10098..11315)
                     /locus_tag="PKN51_03790"
     CDS             complement(10098..11315)
                     /locus_tag="PKN51_03790"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNT84252.1"
                     /translation="MRLNPHLVAMPGDYLFAEVARRVQAYAPSAPRPIIRLGIGDVTQ
                     PLAPAITQAMVEAAQEMGTPEGFHGYPPDQGYPFLQQAIIDADYAPLGVRLELDEVFV
                     TDGAKTDTSALPELLAADARIGVTDPVYPAYIDANAMAGRLGRHEGGAWSALTILPCR
                     AENDFVPELPAQPVDMLYLCYPNNPTGTVLTREQLTRFVRYAQDTGTLILFDAAYKAY
                     ITDPAIPRSIYEIEGADQVAVECCSFSKSAGFTGTRCAYTIIPKALKARIDGQSVSLN
                     ALWRRRVACRQNGVSYPIQRAAAAALSPQGQRQVAAQVQTYMDNAHHMVATLQGAGLQ
                     VFGGVHAPYVWLRIPGQRSSWEFFDHLLHQAQVVGTPGSGFGQAGEGYLRLTAFNTAA
                     NTREALERVLAVL"
     gene            complement(11317..12153)
                     /gene="dapF"
                     /locus_tag="PKN51_03795"
     CDS             complement(11317..12153)
                     /gene="dapF"
                     /locus_tag="PKN51_03795"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNT84253.1"
                     /translation="MKFTKMHGLGNDYVYVNAFDETVADPAAMAVAVSRPHFGVGADG
                     LVLIGPSAEADFAMRMFNADGSEGEMCGNAVRCIGKYVHDRGLTRKTEVRLSTLGGIK
                     TLQLRLKDGSVDQVTVDMGEPAFSPASLPVEWPGERIVNQPLTIDGQTHHITCVSMGN
                     PHAVIFVDDPKAIDVHGIGRQIENHPLFPRRTNVEFVRVRRRDLLEMRVWERGSGETL
                     ACGTGAAASLVAAVLNGLADRSAVVQLTGGDLQVTWDSSDNHVYQTGPASFVFDGVWL
                     GD"
     gene            complement(12166..13581)
                     /gene="glnA"
                     /locus_tag="PKN51_03800"
     CDS             complement(12166..13581)
                     /gene="glnA"
                     /locus_tag="PKN51_03800"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNT84254.1"
                     /translation="MSHTAADVMTMIQEQNIRYIDLRFTDTRGKEQHVTVPVSAFDED
                     KFSSGHPFDGSSIAGWKGIQASDMLLLPDPATANVDPFFDDPTLILTCDVLEPGDGKP
                     YDRDPRSLARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVRWQADMSGCFVKIDSEEAA
                     WNTGTAYEQGNHGHRPGVKGGYFPVPPVDSFQNMRSQMCTLLESLGIPVEVHHHEVAT
                     AGQMELGTKFSTLVQRADWLQLQKYVIHNVAHAHGKTATFMPKPIVGDNGSGMHVHQS
                     VWKDGRNLFAGDGYAGLSEFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVK
                     LAYSARNRSASIRIPYVANPKGRRIEVRFPDPTANPYLAFSALLMAGLDGVENRIHPG
                     EAMDKDLYHLPPEEDALIPTVCHSLDQALEHLDKDRGFLTKGGVFSDAMIDAYLELKM
                     AEVTRFRMTTHPIEFDMYYSL"
     gene            complement(13825..14220)
                     /locus_tag="PKN51_03805"
     CDS             complement(13825..14220)
                     /locus_tag="PKN51_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNT84255.1"
                     /translation="MSFVEHSAAGVAILLWASDPAQPERLATPFFHAAAAAAMDVPVE
                     IYFSAASVQLLAPGVAAALRASPRHDKTILDALREAVTHGARLLACTDALHAHGLAPS
                     GLIPECHGQGGAVQFMARALDTRWRTLVF"
     gene            complement(14217..14684)
                     /locus_tag="PKN51_03810"
     CDS             complement(14217..14684)
                     /locus_tag="PKN51_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HNT84256.1"
                     /translation="MSNAPLQHPADLHYLMEHQVWARLAGDGTATVGITELGIRLSGE
                     IYMCRAKRIGTEVAQGGTLAVVELAKAIVAVKSPVTGTVIEANAALADRPQLVHRDPY
                     GTGWIARLRLADFQADQPALLHGDAVAPAMARHALAHGQQLAGTTRHPSHPAP"
     gene            14782..15261
                     /locus_tag="PKN51_03815"
     CDS             14782..15261
                     /locus_tag="PKN51_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HNT84257.1"
                     /translation="MTKGSRIVLLALACTACTGALAQKIGDVSTAFQWIGPNHKIVVE
                     AYDDPLVQGVTCYVSRARTGGIKGGLGLAEDRAEASIACRQVAPVQFKEPLRREDEVF
                     SERMSILFKRLRVVRMVDAPRNTLVYLTYSDKLIEGSPQNAITAVPIDHATPIPLRP"
     gene            complement(15407..15502)
                     /locus_tag="PKN51_03820"
     tRNA            complement(15407..15502)
                     /locus_tag="PKN51_03820"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15466..15468),aa:Sec,seq:tca)
     gene            complement(15509..17410)
                     /gene="selB"
                     /locus_tag="PKN51_03825"
     CDS             complement(15509..17410)
                     /gene="selB"
                     /locus_tag="PKN51_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNT84258.1"
                     /translation="MIVGTAGHIDHGKTTLVRALTGVDTDRLPEEKKRGISIELGYAF
                     LEPPSGARIGFIDVPGHERLVHTMVAGASGIDFALLLVAADDGPMPQTREHLAVLSLL
                     GLTRGAVVITKADRATPERVQAVREQTRALLAGTTLESAPVLAVSAHTGAGIDDLRAL
                     LFVAAGAEQASSEAEFDSKALRLTIDRAFTLQGTGTVVTGTIHAGSVGMGDELAIVPG
                     TLRARVRSLHAQNQAVARAHAGQRCAVALAGLARDEVTRGQWLTAPAVALASERLDLR
                     LTLWPGEAQALRSGTRVQAHLGAAHVNATVALLDSDALAPGATALAQLLLQAPVGAWH
                     GERVLLRDAAASRTLAGGRVLDPLAPARYRRTPQRLAELSALGLPSAAERLTALLAVA
                     PHGVDLKRFAAAQGLAHPPDQALPAPALVQDGLALGADQAHTLAAAALAALQRFHDQQ
                     PDELGPDSARLRRLSAPRLPAPLWQGLMARLATEGQLVLHGAVVHLPSHGAQLSATEA
                     RIAQKVAPALVQAGFQGAWARDLARDAAEPEPLVRTTLARLARRGELHQIVKDLYYPP
                     DTLQRLAALARQVGAAHQGIVRAADFRDATDLGRKRAIQILEYFDRIGFTRRIGDEHR
                     LRTDCALFS"
     gene            complement(17424..18818)
                     /locus_tag="PKN51_03830"
     CDS             complement(17424..18818)
                     /locus_tag="PKN51_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT84259.1"
                     /translation="MTIDRRHFVAGLAGAATLAGCAAAPPVSGVGVQTAPMPAFIGQS
                     TQTLLPRGKAPRVLVVGGGWGGLTTAANLRKQAPGAEVVLIDRNPQFFSSPLSNMWLV
                     GMVDGELLTQDYLRVSEQLGYRFVQAEVQAIDRAQRRVTTSLGTIDYDWLVLAPGIRY
                     QYEAWFGNDRVAADAARTRFPCAYASNSEFLAIKRSLQDFKGGELVMNLPPPPQRCPP
                     SPYERACLIAWYFKTNKIKGHITILDHKPAITPIGPGYRAAFDELYKDYITYVPNAHV
                     QEVDPFNRRIRTRAGDQRFDHAILMAPHKAGDLAWLAGTVSDRSEGWADVDQVTMQVR
                     GDERTFVIGDAIQAVSSLPFRYYPKSAHIANRLGRIVAYQISERLAGRAPERRLPDNL
                     CYMVVNGTPGQALNVQFDYDFDPEGRVRQVQRDFNERTPALFQDDFRWLDFMFGEMFG
                     GIAPPVRPNPAFRT"
     gene            complement(18823..20310)
                     /gene="selA"
                     /locus_tag="PKN51_03835"
     CDS             complement(18823..20310)
                     /gene="selA"
                     /locus_tag="PKN51_03835"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNT84260.1"
                     /translation="MTAPEQSVVHGTPAPPDAPTTSAPHALPALDRLLRLPAVAALEA
                     EHGRSLLTHEARALLAELRQQALAGRLPAAEIAPDALAGALARRLHDRLAPRLNTVIN
                     LTGTVIHTNLGRALLPAAALAHVQATMAGPNNLEYDLASGGRGDRDSIVEGLLCQLTG
                     AEAATVVNNNAAAVLLILAALARGRQVIVSRGELVEIGGAFRMPDVMTSAGARLVEVG
                     TTNRTHPHDYERAITARTALLMKVHTSNYAVQGFTRAVDEATLAPIAHAHGLPLVTDL
                     GSGALIDMTAYGLPAEPTPQQMLRAGCDLVSFSGDKLLGGPQAGLIVGRRALVERVRK
                     HPMKRALRMSKLPLAALEATLRLYLRPERLARELPTLRLLTRPLADIEATARAVQPAL
                     AAALAPRFTVETVALQGQIGSGSLPVERLPSAGLAIAPVARTGRGRALDELATALRRL
                     PEPVIGRIADDQLILDCRCLEDSTLLTRQLRQLSPQATTVPPATK"
     gene            complement(20310..21302)
                     /gene="fdhE"
                     /locus_tag="PKN51_03840"
     CDS             complement(20310..21302)
                     /gene="fdhE"
                     /locus_tag="PKN51_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HNT84261.1"
                     /translation="MTTTHPPTLPSPEEIALQAGADIAAVRAPQADTLFAERALRLRE
                     LAAGHAMRDYLLLLALVSEAQHERARRYPQVRLPDAAQIDASAATGQPLLAATAWPRE
                     PLWRTELRALLAQVLQQLPGDSPARATVQAVIGLPDQALEQQADRLLAGITLGLDMAA
                     APLIAAGLQLYWAHLVAGTASAHPGLWRHLESRGTCPCCGSLPTSSIRRVGPGMKGQR
                     YLHCTLCQTEWHMVQVMCTHCLGTQGIHYQALQALAAAAPTSERAAVEAETCDHCHHY
                     LKLLHMEREPHADPVADDLATLTLDLLVSDAGFTRHGTNLLLLFGDAEAPPGER"
     gene            complement(21375..22028)
                     /locus_tag="PKN51_03845"
     CDS             complement(21375..22028)
                     /locus_tag="PKN51_03845"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HNT84262.1"
                     /translation="MKPMYLQRYSAGQRSNHWAVVACFVLAAVSGFALFHPALFWLSA
                     LVGGPQWTRILHPFLGAAMCLLFLGMFFMLAGANVWRSEDSQWLGSAGKLVSSEGGHA
                     DMPPVGKYNGGQKLVFWLFALSLLVLLISGLVVVWPQWFGGGASIGMQRMAIVLHAVA
                     AVVLVLAVIVHAYAAFWVKGTLRAMTRGSVSASWARHHHPLWYRERMQNPSSDARVK"
     gene            complement(22025..22933)
                     /gene="fdxH"
                     /locus_tag="PKN51_03850"
     CDS             complement(22025..22933)
                     /gene="fdxH"
                     /locus_tag="PKN51_03850"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HNT84263.1"
                     /translation="MSSLQSQDVVARSATTTPPPRARSSTPQVAKLIDVSKCIGCKAC
                     QVACMEWNDLRDEVGSNIGVYDNPTNLTAQSWTVMRFYEAEPEQDKLEWLIRKDGCMH
                     CEDPGCLKACPSPGAIVKYANGIVDFISEHCIGCGYCIKGCPFDVPRISPQDNKAYKC
                     TLCSDRVGVGLEPACVKTCPTGAISFGTKDDMVSYGQQRAADLKERGHQNAGLYNPPG
                     VGGTHVMYVLKHADRPELAGLPKEPSISPWVSVWKGWTKPVAMAAMAGAALAGWFHYL
                     RKGPLEVPEDAEATAGGATRTEGESS"
     gene            complement(22937..26017)
                     /gene="fdnG"
                     /locus_tag="PKN51_03855"
     CDS             complement(22937..26017)
                     /gene="fdnG"
                     /locus_tag="PKN51_03855"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016497753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(25430..25432),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HNT84264.1"
                     /translation="MDMNRRQFFRVSGAGLAASSLVALGFSPTAALAETRAFKLTRAS
                     VSRSTCPYCSVSCGMLIYKLGDTAKNVREHVIHVEGDPDNPVNRGTLCPKGAGVMDMI
                     NSPGRVIYPSVREAGSKEWKRISWDEALTRVARHMKADRDANFQAKNDAGTTVNRWPT
                     TGFLVCSSSSNESSYLSVKVARALGMVGIDTQARVUHAPTVTSLGPTFGRGAMTNSWT
                     DIRNTDLILVMGGNAAEAHPVGFRWAVDAMEKRKARLISVDPRFNRTSSVADLYAPIR
                     NGTDIAFLGGVINWLLSHDKIHHDYVKFNTDATFLLKPDFKLENGIFSGYDEAKRKYD
                     KASWGYQLDENGHVKVDDSLQDPLCVYQQLKKHYSRYTPEMVSRICGTPQDKFLKVCE
                     MLGEMSAPDKTSTILYALGWTQHSVGSQNIRTMAMIQLLLGNMGRPGGGVNALRGHSN
                     VQGVTDMNAYAEVLSGYLSAPTDADDTFEKYLAKRTPKPMRPNQFNYWSNFPRFHISL
                     MKSYYGKAATADNQYCYDWVPKLAGGFDALHMFELMHQGKMTGFISQGFNPLATVPNK
                     NKLSAALSKLKYLVIIDPLETETGEFWQNHGPFNDVKPESIQTEVFRLPSACFAEEEG
                     SFTNSSRIAIWKERALEPPGEAKVDSEIMARLFLKLRQMYAKDGGAFPDAIAGLAWDY
                     VQPEAPSATEVLREINGKALSDVLAPPDPNNPTAPRQVLVKAGQQVPGFAMLRDDGST
                     ACGNWLYAGCWSEAGNLTARRDVSDPTGLGVYQNWGFSWPANRRILYNRASADKNGKP
                     WSENKKYVYWNGKAWAGADVPDMRPNAAPEENVGSFIMNPEGVARLHAIGMADGPFPE
                     HYEPFETPIGVNPLHPDNPKAISNPAARVFKGDLEAFGKKEEFPYSATTYRLTEHFHT
                     WTKHARLNAIVQPESFVEISEELAKEKNIRHGDQVKVSSNRGHIVTRAVVTKRIKPWN
                     VNDQKVHLVGIPFHWGFKGQTKNGYLANTLSPFVGDANSQTPEFKAFLVNIEKV"
     gene            complement(26196..28025)
                     /locus_tag="PKN51_03860"
     CDS             complement(26196..28025)
                     /locus_tag="PKN51_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HNT84265.1"
                     /translation="MALPHRPTTLAALAAATLLAGCASSLNYTPPATPVTPEGGSGWT
                     DKPGWATHKFAVAAANPLATDAGYQVLKAGGSALDAAIAVQMVLTLVEPQSSGIGGGA
                     FLLHAAGGDVTAWDGRETAPAAVDDKLFLGADGKPLPFHDGVVGGRSVGVPGTVRMLE
                     QAHRQHGRLAWAELFKPAIVLAEQGFAVSPRLHTLLKNEQHLKKDPVAARHFYDAAGN
                     PWPVGHVLKNPELAAVLKGIAARGSTALLEGPVAQAIVDKVQKHPTNPGKLTLADLAG
                     YAPKRRAPLCHDLRVPASGKAYEVCGFPPPSSGAIAVGQILGILAHTPAASMAAGADG
                     LPTADWLHYYMEASRLAFADRALYVADPDFVQPPAGSWMSLLDPAYLKSRAALIGTQS
                     LKVAPPGKPVGTRTSFAPMASQPEYGTSHISIVDPFGNALAMTTTIEDQFGARQMVKG
                     FLLNNELTDFSFAPTDAQGQPIANRVQPGKRPRSSMAPTLVFEKTTDGRRGPLVMSAG
                     SPGGALIIHYTAKTLHGVLGWGLLPQQAINLPNFGSLNGPSLLEEKKFPAATVEALRS
                     RGAEVREMNLTSGLQAITRGTVHGQPTWLGGADPRREGVVMGD"
     gene            complement(28214..28972)
                     /locus_tag="PKN51_03865"
     CDS             complement(28214..28972)
                     /locus_tag="PKN51_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNT84266.1"
                     /translation="MQIKDKVFIVTGGASGLGEGTARMLAARGGKVVIADMQVDKGEA
                     VAREIGGAFVKCDVSSEADGQAVVAKAVSMGKLMGLVNCAGIATAERTVGKNGPHRLD
                     GYTRTIMVNLVGTFNMIRLAADAMSRNEPEPTGERGVLISTASVAAYDGQIGQAAYSA
                     SKGGVVGMTLPIARDLAKSGIRNMTIAPGIFGTPMLFGMPKEVQDALAAGVPFPSRLG
                     TPEDYAKLACHIIENDMLNGEVIRLDGAIRLAPK"
     gene            29099..29422
                     /locus_tag="PKN51_03870"
     CDS             29099..29422
                     /locus_tag="PKN51_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84267.1"
                     /translation="MCIHCSPHDRRDDDELRPTAEALLAATLALMTGVAEAAPGAAGV
                     RPMSLKVLANLDELARHPGLSRSMRQLAAHLGLHWQQLVQTARGEARAAGERARWVPA
                     STVVQ"
     gene            29419..29835
                     /locus_tag="PKN51_03875"
     CDS             29419..29835
                     /locus_tag="PKN51_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84268.1"
                     /translation="MSALEPGALQREHQLLLRHLGRVQQRVSEQMLAQAQRCAALEAE
                     LMQWRARWIVATTRRLWGLGGFDQPPAPARAVPADRSAWASATEVLCQTGCAGHAHPW
                     RDEQGRCALTASDCTRVPPVAQDASAGAVARVFSRP"
     gene            complement(29797..30888)
                     /locus_tag="PKN51_03880"
     CDS             complement(29797..30888)
                     /locus_tag="PKN51_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT84269.1"
                     /translation="MNPHRPAPTLDTDVAIIGAGPVGLFAVFQLGLLELRAHVIDALP
                     HAGGQPVELYADKPIYDIAAVPVCSGQTLTDALLRQIAPFAPQWHLGHLVESLQRLPD
                     GRFLLQTEPGRRLRARSVFIAAGVGAFLPRRLKVAGLEAFERRQLFHQAPADAALAGQ
                     HVVVVGGGEPALTQAIRLATLAAAAPASVTLLHRRDGFQAAPATQARVRALCAAGSMR
                     FRVGQVVGFEQAAGRLSALQVMGPDGSTQPLPLELLLVQQGVSPRLGPITAWQLALER
                     KQLVVDAATFQTSEPGIFAIGDINTYPGKKKLMVCGFHEATLAAYAAREHIHPHDPLP
                     FEYTTSSARLQRLLGVASGAREHAGDGAG"
BASE COUNT         4466 a        10168 c        11236 g         5226 t
ORIGIN      
        1 gccttcgcgg atggcccaga cgaccaggct ctggccgcga cgcatgtcgc cggcggcaaa
       61 cacgcgctcc acactggtgc ggtaaccgtg gaccacatcg gtgccggcgc gggcgttgcc
      121 gcgcgcatcc ttgtcgatgc cgaaggcgtc cagcacacgg ccgaccgggt gcacgaagcc
      181 catcgccagc agcaccagat cggccttcca ggtcttttcg gtgcccggga cctcggtcat
      241 cttgccgccg gcccattcga cctcgaccgt cttcagcccg acgatgcgtc ccttgtcctt
      301 gccggtgccg gcgacgaatt ccttggtggc gatggcgaac tggcgctcgc aaccttcttc
      361 gtggctggaa ctggtgcgca ggcggtaggg ccagtacggc caggtcaggg gcttgtcctc
      421 ctgctcgggc ggcatgggca tgagctcgaa ctgggtgacg ctggcagcgc cctgccggtt
      481 gctggtgccg acacagtcgc tgccggtgtc gccaccaccg atgacgatca cgtgcttgcc
      541 cttggcatga atctgcccct tgaggttgtc accggccacc acccggttct gctgcggcag
      601 gaactccatc gcgaagtgca cgccgtcgag cgcgcgaccc ggcaccggca ggtcacggct
      661 gacctcactg ccaccggtca gcagcaccgc atcgaactcg gccagcaact gctcggcgct
      721 gagcgtttgt tcgccgtgac gggtgatttt gctgtccggg tccagctcgc cgaccagcac
      781 gccggtgcgg aacaccacac cctcggcgct catctgcgcg atgcggcggt cgatgtggcc
      841 cttgtccagc ttgaaatccg gaatgccata gcgcagcagg ccgccaggct gggcgttctt
      901 ttcgaacacc gtcaccgcgt gcccggcgcg ggcaagctgc tgggccgccg ccagcccggc
      961 cggtccggag ccgatcaccg ccaccttctt gctggtcttg atggccgccg gctgcggcgt
     1021 cacccagcct tcggcccagg cgcggtcgat gatggcgtgc tcgatgctct tgatgccgac
     1081 ggcgtcgtca ttgatgttga gcgtgcaggc ggcttcgcag ggtgccgggc agacgcgccc
     1141 ggtgaactcg gggaagttgt tggtctggtg cagcacgtcg atggcacggc gccagtcgtc
     1201 gtgatagatg aggtcgttga agtccggaat caggttgttg accgggcagc cgtggttgca
     1261 gaacggggtg ccgcaatcca tgcagcgtgc cgcctgcgtg cgcgcctgcg cgccgtccag
     1321 gccgatgacg aactccttcc aggtcttcag tcgcgccggc acggcctcgc tgcgttcctc
     1381 gatgcgttcg aattccagaa agccggtgat ctttcccatg atgctgtctt gcttgaatgg
     1441 ttgtcattga cccgatgcca gcgcggcgct ggcctttgcc ttcaccagcg cgctgtcggc
     1501 ggtggcgatg gcggcctgtt cggcatgcgc cgagagcacc cgctggtact cacgcggcat
     1561 caccttgaca aagcgtgcgc gtgaatcggc ccagtgatcg aggatttcgc gcgcgcgcag
     1621 cgaacccgtc cagcgcagat gctcctcgat cagcttgcgc agcaggtcct catcggccat
     1681 ggcgcggtgc cagccggcgg cgatgccttc ctgggcgtcg cgcggcggca ccttctcgag
     1741 ggccaccatg ctggtgttgc aacgttcgct gaagcgaccg tcctcgtcga acacgtaggc
     1801 cacgccaccg ctcatgccgg cggcgaagtt gcggccggta ctgcccagca ccaccacggt
     1861 gccgccggtc atgtattcac agccgtggtc gccggtgcct tccaccacgg cggtggcgcc
     1921 cgacaggcgc accgcgaagc gttcgcccgc cacgccgcgg aagaaggctt cgccgctggt
     1981 ggcgccgtag agcgcggtgt tgccgacgat gatgttgcgg gtggcgtcgc cgcggaagtc
     2041 gatgctcggc cgcaccacga tgcgtccgcc cgacaggccc ttgccggtgt agtcgttggc
     2101 gtcaccgatc agatgcaggg tgacgccgcg cgccaggaag gcgccgaagc tctggccacc
     2161 cgtgccttcg agctgcatca cgacggtgtg gtcgggcagc ccttcggggt ggcggcgcac
     2221 cagctcgccc gacagcatgg cgcccacgct gcgatgcacg ttgcgcacct cgtccatcaa
     2281 ctggacccgc tcgccgcgct ccagcgccgg ctggcaacgc tcgatgaact tgtggtccag
     2341 ggcgcgggcc aggccgtggt cctgcgtttc cgtctgcagc cgcggcactt ccgacggcac
     2401 cgccggttgg tggaacacac ggctgaagtc cagcccctgg gccttccagt gggtgatggc
     2461 tctgcgcgta tcgagcagat cggcgcgccc gatcagttcg tcgaagcggc gcaggcccag
     2521 ctgcgccatg atggtgcgca cttcttcggc gacgaagaag aagtagttga tgacgtgctc
     2581 gggcttgccc gaaaacctgc ggcgcagcac cggatcctgg gtggccacgc cgaccgggca
     2641 ggtgttgagg tggcacttgc gcatcatgat gcagccctcg gccaccaggg gtgcggtggc
     2701 gaatccgaac tcgtcggcgc ccagcagcgc gccgatcacg acgtcacgcc cggttttcat
     2761 ttgcccgtcg gcctgcacgc ggatgcggct gcgcaggcgg ttgagcacca gggtctgctg
     2821 ggtctcggcc aaccccagct cccacggcgt gccggcatgg cggatgctgg accagggcga
     2881 ggcaccggtg ccgccgtcgt ggccggcaat caccacatgg tcggccttgc acttggcgac
     2941 gccggcggcg atggtgccga cgcccacttc gctgaccagt ttgacgctga tggaggcctg
     3001 cgggttgacg ttcttcaggt cgtggatgag ctgggccagg tcttcgatgg agtagatgtc
     3061 atggtgcggt ggcgggctga tcagacccac cccggggacg ctgtagcgca gaaagccgat
     3121 gtactccgac accttgccac cgggcagttg gccgccttcg ccgggcttgg ccccctgggc
     3181 catcttgatc tggatctggt cggccgaaac caggtactcg gccgtcacac caaaacgccc
     3241 ggaagccacc tgcttgatcc ggctgcgcag gctgtcgccg gcctgcatgg cgtagtcgga
     3301 ttcgatgacg cggctgccga tcacctcgct gacgcgggtg ccggcggcga tcgggatgcc
     3361 cttgagttcg ttgcgatagc gcgccgggtc ttcgccgcct tcgccggtgt tgctcttgcc
     3421 gccgatgcgg ttcatggcca gcgccagcgt ggtgtgggct tcggtgctga tcgaaccgag
     3481 cgacatggcg ccggtggcaa agcgcttgac gatctcggcg gcgggctcca cctgatcgag
     3541 cgggatggcg cgggccgggt cgaccttgaa ctcgaacagg ccgcgcagcg tcatgtggcg
     3601 gcggctctgg tcgttgatga tctgcgcgta ctccttgtag gtgtcccact tgccgccgcg
     3661 cacgctgtgc tgaagcttgg cgatggcgtc cggcgtccac atgtgttcct cgccgcgggt
     3721 gcgccaggcc aggtcgcccc cggcgtcgag catgttctgc agcgtcgggt cgtcgccgaa
     3781 cgcggccagg tgctggcgca gcgcttcttc ggccacttcg aacacaccga tgccgcccac
     3841 ctgggtggcg gtgccacgga agtacttggc gacaaagtcc tctttcaggc cgatggcttc
     3901 gaagatctgg gatccgcagt acgacatgta ggtgctgatg cccatcttgg acatgatctt
     3961 ggacagcccc ttgccaatcg ccttgacgta gttctggatc gccttcttgg gcgaaggccc
     4021 ctcgggcgtc gcttcgtgca gggcggtcag cgtttccatc gccaggtacg ggtgcaccgc
     4081 ttcggcgccg tagccggcca acaccgcaaa gtggtgcact tcgcgcgcac tgccggtttc
     4141 caccaccagg ccggcggtgg tgcgcagtcc cgcatgcacc aggtggtggt gaatggccga
     4201 cagggccagc aacgccggaa tggcgatgtg gtctcggtcc atgcggcgat cgctgatgac
     4261 gaggatgttg tgcccgccac ggatggcgtc aagcgcctcg gcacacagcg aggccagctt
     4321 ggcttccacg ccttcgcggc cccaggacag cgggtaggtg atgtcgatcg tgtaggtctt
     4381 gaacttgccg ccggtgtgcc gctcgatcgc gcgcaggcgc gccatgtcgt cgaagtccag
     4441 aatgggctgc tcgacctcca gccgcatctg cgggttgacc gcattgatgt ccagcaggtc
     4501 cggcttgggg ccgatgaagc tgtgcagcga catcacgatg gcttcgcgga tcgggtcgat
     4561 cggcgggttg gtgacctgag cgaacaactg cttgaaatag ttgtacagcg gcttgttctt
     4621 gtccgacagc accgccagcg gtgtgtcgtt gcccatcgag ccaatggctt cctcgccctg
     4681 ctgggccatg ggcagcagct ggaacttgat gtcctcctgc gtgtagccga aggcctgctg
     4741 gcgatcgagc agcgtttcgg tgtagctggg gcgcgcgccg tcgctcgggg tgtcggccag
     4801 gcgcacgcga acgttttcga tccactgccg gtatgggcgg gcgttggaca gcagggtctt
     4861 gagctcttcg tcgttgacga tgcgaccctg ctccagatcg atcaggaaca tcttgccggg
     4921 ctgcaggcgc cacttgctga cgatccggct ctcggggatc ggcagcacgc cgctttcact
     4981 ggccatgatg acgtggtcgt cgtcggtgac gatgtagcgc gccggtcgca ggccgttacg
     5041 gtcgagcgtg gcaccgatct gcttgccgtc ggtgaacacc atggcggccg ggccgtccca
     5101 cggctccagc atggcggcgt ggtattcgta gaacgcgcgc cggcgttcgt ccatggtctc
     5161 gtgctgttcc caggcctcgg ggatcatcat catcatggcc tgggccagcg ggtagccggc
     5221 cgacaccaga agttccagcg cgttgtcgaa ggtggcggtg tcgctctggc cttcgaagga
     5281 gatcgggtag agcttctgca ggtcctcgcc cagcacgggc gagcgcatca cgccctcgcg
     5341 tgcgcgcatc cagttgaaat tgcccttgac ggtgttgatc tcgccgttgt gggcgacgaa
     5401 gcggtagggg tgggccagcg gccattcggg gaaggtgttg gtcgaaaaac gctggtgcac
     5461 cagcgccagt gccgagaccg tgcgcgggtc ggccaggtcc ctgtagtact gacccacctg
     5521 gtcggccagc agcaggccct tgtagatgac ggtgcggcag ctcatgctgg gcacgtagta
     5581 ttcgtggctg tgtgtcagcc gcagccgctg gatggcggcg ctggcggtct tgcggatcac
     5641 atagagcttg cgctccagcg catccggcac gatgacgtcg cgaccgcggc cgatgaagat
     5701 ctgacggatc accggctcct tggcgcgcac cgtcgggctc atcggcatgt cgcggtcgac
     5761 cggcacgtcg cgccagccga gcaggacctg accttcgtcg cgcacggcgc gctcgagttc
     5821 gtgctcgcag gccaggcgcg aagcgtgttc cttgggcagg aagatcatgc cgacgccgta
     5881 ctcgcccggc gggggcaggg tgacgccctg tgccgccatc tcggcgcggt agtactcgtc
     5941 ggggatctgg atcaggatgc cggcgccgtc gcccatcagc ttgtcggcgc ccacggcgcc
     6001 tcgatggtca aggttgtgca ggatgcgcaa cccctgggtg acgacgtcgt gcgtcttgtg
     6061 gcccttgatg tgggcgatga agccgacccc gcaggcgtcg tgttcgtgcg tgggctggta
     6121 cagaccgccg gcagcggcgg cgaggatttc ttcgtggggg gcggggagcg ggtggctcaa
     6181 ggcttgactc caacgtgggc gtgatgaggc atggaggcta gcatgcgcaa acggggacta
     6241 caagaaatat aaaacggggt cggaacataa ataaatgtca aaaggttaga tttcataaaa
     6301 ataggggaca taacaaataa gggtgtacca atggtgcgcc gcctgatctc gggtgcgacc
     6361 gatggtgacg taagtgttgg ttatgaaacg gcttttttgt cgaccgccgc tggtgttcac
     6421 caggtgccgc cgacggacgc gggcgctcag cgcgggttgc tggcggcgcg cttggggcgc
     6481 ccaggtcggc gtggggccgc gggtcgcccg gtggcatccg cgctgcggat gaattcgggc
     6541 gagcccagcg cccagccgcc cagcaccagg cggcgcagcc gcgcctccgt ggtgccggcc
     6601 acgccgcgtg caaggcgctc ccgccaggcc gattcgcgtt cgaagggggt gttgccgagt
     6661 tgccagtatt cgggcggatc ggtgatcagg gggttgggct gcgtcccggc atggtgcggc
     6721 gcactggtga tgccgggtgt cggcgtggcc tgctcgatcc aggtcatggc ggtcagggtg
     6781 tgttccccgg gttccacggc actggtgcga aaccgcccgt cccacagcgg gccgctgcgt
     6841 gcgtgccgcc ggttgtaggc cgtcacgtag cgccgaccga tggcctgcat cagccgcgcc
     6901 agcgcgtcgg gctgggccgg cgtgaccaga aacagcaact cgtggtcgcg cagtgcgaag
     6961 gcgtgcaact gcaccgcttc cgtttccagg gtggtgcgca gcgcgaccat gaactgcgca
     7021 cggtcctcgt cgtcgacgaa ggcgggacgg tggtcgagcg tgcgttggag caccagatgg
     7081 ggctggccgg gcagcacaag gcggggcagg cgcgccatgg gaagcgttca gaaggtggac
     7141 aagaaggggt tcgctcccac ttgacttgtt cagggcgcgg gtctgaccgt gggctcgcgc
     7201 cgcctgcgcg tgtgggggcg tgccgcaggg cgtggcgagc gttcagtcat cgagtcccag
     7261 ctcctggatc ttgcgggtga tggtgttgcg tccgatgccg agcttctggg cggcctcggc
     7321 gcggcgcccg tgggtcaacc ccagggcggt gtgcagcagc gccgcttcga agcgacccgc
     7381 caggctgtcc cacaccccgg actcaccggc cgccagccgg gtgcgtgcct cctgagccag
     7441 ggccaccgtc cagtcggcgg gcgcggcggc gctgggtggg gcgtcggggt ggggtggcgt
     7501 gatggccgcc gtcgccttct cttcgcccgt cagcggacgt ggctcaccac gcggcagcag
     7561 gtcggccggc aggtcttccg cctcgatccg ctggccaggg gccatcaccg tcagccagcg
     7621 gcacaggttt tccagctggc gcacgttgcc gggaaagtcg aactgcgtca gccgttccag
     7681 cgcggcgggg ctgatgtgct tgctctccac ccccagctcg cgtgcgctgc gggcaagaaa
     7741 atggcgcgtc agcaggggga tgtcttcgcg tcgctcattg agcggcggca gccgcagtcg
     7801 aatgacgttg aggcggtgga acaggtcttc ccggaaggtg ccctggcgca cgcgctcttc
     7861 gagatcctgg tgcgtggcgg cgatcacccg ggtgtcggcc cgcagcggct ggtggccgcc
     7921 gacccgatag aactgcccgt cactcagcac gcgcagcagg cgggtctgca actccagcgg
     7981 catgtcgccg atttcgtcca ggaacagggt gccgccggcg gcctgctcga agcgaccgcg
     8041 ccgcatggcc tgcgcgccgg tgaaggcgcc gcgttcatgg ccgaacagct cgctctccag
     8101 caggtccttg ggaatggcgg ccgtgttgat ggccacgaac ggcccgcgcg cccgcggact
     8161 gtgacggtgc agggccagcg ccaccagctc cttgccggtg ccgctggcgc cggtgatgag
     8221 caccgtgctt tcactttggc tgagccgtcc gatggcgcga aacacctcct gcatggccgg
     8281 cgcctcgccc agcatcaggg gctgctgatc gctgtcgtcg gcgtgcggct gatcgcgcag
     8341 gctgtggtct tcggcggcgc ggcgaatcag ttcgaccgcg cggttgacgt cgaagggttt
     8401 tggcaggtac tcgaaggcgc cgctgcgaaa cgcgcccacc gcgctgtcga ggtcgctgta
     8461 ggcggtcatg atgatgaccg gcagctgggg gtggcggcgc ttggcttcgg tcagcagctc
     8521 gatgccgctg ctgcccggca tgcggatgtc ggtcaccagc acctggggct cgacccccgc
     8581 atcgaactcg ttgagcacgt cctgcggtcg gctgaagctg cgcacgggta ggtgctcgcg
     8641 gctcagcgcg cgttccagta cgaaacggat ggattgatcg tcgtcgacga cccagatcgg
     8701 cttcatggcg ggccctcgat gtcgcggcag gcggtagggt cgggcagggg gatcagcagc
     8761 gtaaagcggg tggcgcccgg ctggctttcg cattcgatcg tgccgccatg ctgttgaatg
     8821 aacccctgcg ccagcgtcag acccagcccg ctgccggcgg catggccgga caccagcggc
     8881 tggaagatgc gctcccgcag ggcggggggg atgccggggc cgttgtcctc cacctgcagc
     8941 tggatcgcca ggcggaagcg ccggccgttg agcgtgacct gacgcgcgat gcgggtgcgc
     9001 agcacgatgc gcgcatcgcc ggtgacgatg cgcgcggcaa gcgcctgcgc ggcgttgcgc
     9061 acgatgttga gcagcgcctg gatcagccgc tcgcggtcgc cgcgcagctc cggcagcgag
     9121 gtgtcgtagt cgcgctgcac ctgcaggccg cgcggaaact cggccagcac cagcgcacgc
     9181 acgcgctcgc agacctcgtg gatgttgaag tcgctccact gcggcatgcc gcggtgcggt
     9241 gccagcagcc ggtcgaccag cgcctgcagc cggtcggcct cgcggatgat gacctcggtg
     9301 tactcgccgt actcggggga ggtgaattcc atcgccagca actgggcggc gccgcggatg
     9361 ccgcccagcg ggttcttgat ctcatgggcc agattgcgca gcagttcctg gtgggcctgg
     9421 atcaggtcct gtgcgcgctc ctcgcggtcg agctgcacct gccgttcgat ctcgatcatc
     9481 tcgaccatca ccgcgccctc cgggccgccg cgtcctaccg gggtcaccac cacatgcacc
     9541 ggcaattcac ccgcgccggg gggctggcgc aacagggcgc cttcgaagcg gctgaccgcc
     9601 aggtgatggc tggcggtgct gtgaagcgcc gcctgcagcg gcgccgggtc gacgaaccaa
     9661 tcgagcgcgc gcgcctgcac cagcttgcgg cgcgattgcc gaaccgcgtg ctccagcgcc
     9721 gcgttgacca gcaggcaggt gccatcgacc cgcaccagcg ccaccatcag ggccagacga
     9781 tcgtaggcct cctggtcctg tggggagatc gcagtgggtg ccgcctccgc accagccgcc
     9841 acgggagcag gcggctcagg gcttggggtc gacctggtca tgacgaaggc agtgcgagtc
     9901 ggatccaagg ggcgtggtgc ctgggcgtca gcggcaccgg gttcgggggc agggcatgtc
     9961 agtgtagcgg gcgctgctgg cctggccggc acccgccgtg cccgcccccg ggggtgcagg
    10021 ggcggcggcg acagggggcg tcgcgccgct gctccggtct gcccgttcgg gcgtgccatg
    10081 ggcgccccgc cacagtgtca cagcaccgcc agcacgcgct cgagcgcttc gcgcgtgttg
    10141 gcagcggtgt tgaacgcggt caggcgcagg tagccttcgc cggcctggcc aaagcctgac
    10201 ccgggggtgc cgaccacctg agcctggtgc agcagatggt cgaagaactc ccacgagctg
    10261 cgctggcccg gaatcctgag ccacacgtag ggtgcgtgca caccgccgaa cacctgcagg
    10321 cccgcgccct ggagtgtcgc caccatgtgg tgggcgttgt ccatgtaggt ctgcacctgg
    10381 gccgccacct ggcgctgccc ctgcggcgac agggccgccg cggcggcgcg ctggatcggg
    10441 taggacacgc cgttctgccg gcacgccacg cggcgacgcc acagcgcgtt gagcgacacc
    10501 gactggccgt cgatgcgggc cttgagcgcc ttggggatga tggtgtaggc gcagcgcgtg
    10561 ccggtgaacc cggccgactt ggagaacgaa cagcactcca ccgccacctg gtcggcgccc
    10621 tcgatttcgt agatcgagcg cgggatcgcc gggtcggtga tgtaggcctt gtaggcggcg
    10681 tcaaacagga tcagcgtgcc ggtgtcctgg gcgtatctga cgaagcgggt cagctgctcg
    10741 cgggtcagca cggtgccggt ggggttgttg gggtagcaca ggtagagcat gtccaccggc
    10801 tgtgccggca gctcgggcac gaagtcgttc tcggcccggc acggcaggat ggtcagtgcg
    10861 ctccatgcgc cgccctcatg gcgtccgagc cggccggcca tggcgttggc gtcgatgtag
    10921 gccggataga ccgggtcggt gacgccgatg cgcgcgtccg cggccagcag ctcgggcagg
    10981 gccgaggtgt cggtcttggc gccgtcggtg acgaacacct cgtccagctc cagccgcacg
    11041 cccagggggg cgtagtcggc atcgatgatg gcctgctgca ggaacgggta gccctgatcg
    11101 ggcgggtagc catgaaagcc ctcgggcgtg cccatttcct gggccgcctc gaccatggcc
    11161 tgagtgatgg cgggggccag gggctgcgtc acgtcgccga tgcccaggcg gatgatcgga
    11221 cgcggtgccg agggcgcata ggcctggacg cggcgcgcca cttcggcaaa caggtagtcg
    11281 ccgggcatgg cgaccaggtg ggggttcaga cgcatttcaa tctccgagcc acacgccatc
    11341 gaacacgaag ctggcggggc ctgtctggta aacgtggttg tcgctgctgt cccaggtgac
    11401 ctgcagatcg ccgccggtca gctgcaccac cgcgctgcga tcggccaggc cgttcagcac
    11461 ggcggccacc aggctggccg cggcgccggt gccgcaggcc agggtttcac cgctgccgcg
    11521 ctcccatacc cgcatttcca acaggtcgcg ccgccgcacg cgcacgaact cgacgttggt
    11581 gcgccgcggg aacagcgggt ggttctcgat ctgccggccg atgccgtgca cgtcgatcgc
    11641 cttcgggtcg tcgacgaaga tgaccgcatg ggggttgccc atgctgacgc aggtgatgtg
    11701 gtgggtctgg ccgtcgatcg tcagcggctg gttgacgatg cgctcgccgg gccactcgac
    11761 cgggaggctg gccgggctga acgccggctc acccatgtcc accgtcacct ggtcgacgct
    11821 gccatccttc agccgcagtt gcagggtctt gatgccgccc agggtcgaca gcctgacttc
    11881 ggtcttgcgc gtcagcccgc ggtcgtgcac gtacttgccg atgcagcgca cggcgttgcc
    11941 gcacatttcg ccttcggagc cgtcggcgtt gaacatgcgc atcgcgaagt cggcctccgc
    12001 cgagggcccg atcagcacca gcccgtcggc gccgacgcca aagtgcgggc gtgacacggc
    12061 gacggccatg gccgccgggt cggccacggt ttcgtcaaag gcattgacgt agacgtagtc
    12121 gttgcccagg ccatgcatct tggtgaattt catgccaccc gttccttaca ggctgtagta
    12181 catgtcgaac tcgatcgggt gggtggtcat gcgaaagcgc gtgacttcgg ccatcttgag
    12241 ttcgaggtag gcgtcgatca tggcgtcgga gaacacaccg cccttggtca ggaagccgcg
    12301 gtccttgtcc aggtgctcca gcgcctgatc caggctgtgg cacacggtgg ggatcagcgc
    12361 gtcctcttcg ggcggcaggt ggtacaggtc cttgtccatc gcctcgccgg ggtggatgcg
    12421 gttttccacg ccgtccagac ccgccatcag cagcgccgag aaggccaggt aggggttggc
    12481 ggtggggtcg gggaagcgca cctcgatgcg ccggcccttg gggttggcca cgtaagggat
    12541 gcggatcgag gccgagcggt tgcgcgcgct gtaggccagc ttcaccggcg cttcgaagcc
    12601 gggcaccagg cgcttgtagc tgttggtgcc ggggttggtg atggcgttga gcgcacgggc
    12661 gtgcttgatg atgccgccga tgtagtacag cgcaaactcg ctcaggccgg cgtagccgtc
    12721 gccggcgaac aggttgcggc cgtccttcca caccgactgg tgcacgtgca tgcccgagcc
    12781 gttgtcgccg acgatgggct tgggcatgaa ggtggccgtc ttgccatggg cgtgggccac
    12841 gttgtggatc acgtacttct gcaactgcag ccagtcggcg cgctgcacca gggtgctgaa
    12901 cttggtgccc agctccatct gaccagcggt ggccacctcg tggtggtgca cctcgaccgg
    12961 gatgcccagc gattcgagca gcgtgcacat ctgcgagcgc atgttctgga agctgtccac
    13021 cggcggcacc gggaagtagc cgcccttgac gcccgggcgg tggccgtggt tgccctgctc
    13081 gtaggcggtg ccggtgttcc aggcggcttc ctcggagtcg atcttgacga agcagcccga
    13141 catgtcggcc tgccagcgca cgccgtcgaa gacgaagaac tcgggctcgg ggccgaagta
    13201 ggcggtgtcg ccaatgccgc tgctcttgag gtaggcctcg gcgcgccggg ccagcgagcg
    13261 cgggtcgcgg tcgtagggct tgccgtcgcc cggctccagc acgtcgcagg tcaggatcag
    13321 ggtggggtcg tcgaagaacg ggtcgacgtt ggcggtggca gggtcgggca gcagcagcat
    13381 gtcggacgcc tggatgcctt tccagccggc gatggacgag ccgtcgaacg ggtgcccgct
    13441 gctgaacttg tcttcgtcga aggcagacac cgggaccgtg acgtgctgct ccttgccccg
    13501 ggtgtcggtg aagcgcagat cgatgtagcg gatgttctgc tcctggatca tcgtcatgac
    13561 gtcggcggcg gtgtggctca ttgggttggc tccatgaagg gttgggatgc cagggttcaa
    13621 gcagaaaccg tgccagcttc tgtggtgcct gtgctgccac gccacgcctg cggtgcgtgc
    13681 gacatgtggg tgcgcgttgc accacaaaca tgcgcggagc ccggatatgg tgcaccaaaa
    13741 cggtgcgtcg caccagtgtg gtgcccgaaa ccggtgcagc gatcggcggc tgtgcgaggt
    13801 ggcggggttg tgccggaccc ggggtcagaa caccagggtg cgccagcgtg tgtccagcgc
    13861 ccgcgccatg aactgcaccg cgccgccctg gccgtggcat tcggggatca gaccggacgg
    13921 tgccaggcca tgggcgtgca gcgcgtcggt gcaggccagc aggcgcgcgc cgtgggtgac
    13981 cgcctcgcgc aacgcgtcca ggatggtctt gtcgtgccgc gggctggcgc gcagggcggc
    14041 ggccacgccc ggggccagca gctgcacgct ggcggcgctg aagtaaattt ccaccggcac
    14101 gtccatggcg gccgcggcgg cggcgtgaaa gaacggcgtg gccagccgtt cgggctgggc
    14161 cgggtcgctg gcccacagca gaatggccac gccggcggcg ctgtgttcga cgaagctcat
    14221 ggtgcggggt ggctcgggtg gcgggtggtg ccagcgagtt gctggccatg cgccagggcg
    14281 tggcgcgcca tggccggcgc cacggcgtca ccgtgcagca gggccggctg gtcggcctgg
    14341 aagtcggcca gccgcagtcg ggcgatccag ccggtgccgt aggggtcgcg gtgcaccagt
    14401 tgggggcgat cggccagcgc cgcgttggct tcgatcacgg tgccggtgac cggcgacttg
    14461 accgccacga tggccttggc gagctccacc acggccagcg tgccgccctg tgccacctcg
    14521 gtaccgatgc gcttggcgcg gcacatgtag atctcgcccg acagccggat gcccagctcg
    14581 gtgatgccca cggtggccgt accgtcgccg gccagacggg cccacacctg gtgctccatc
    14641 aggtagtgca ggtcggcagg gtgctgcagc ggcgcgttcg acatgagggg tattctggca
    14701 ggccctggga cactattaaa accatagctg ctggcgcaaa ctacacttgc gccaacaaca
    14761 ttttttattg gaattgaagc gatgaccaag ggttctcgga tcgtgctgct ggcgctggct
    14821 tgcaccgcct gcaccggcgc gctggcgcag aagatcggcg acgtcagcac cgccttccag
    14881 tggatcggcc ccaaccacaa gatcgtcgtc gaggcctacg acgacccgct ggtgcagggg
    14941 gtgacctgct acgtttcgcg cgcgcgcacc ggcggcatca agggcgggct ggggctggcc
    15001 gaagaccgtg ccgaggcttc catcgcctgt cgccaggtgg cgccggtgca gttcaaggag
    15061 ccgctgcggc gcgaggacga ggtgttcagc gaacgcatgt cgatcctgtt caagcgcctg
    15121 cgcgtggtgc gcatggtgga tgcgccgcgc aacacgctgg tgtatctgac ctactccgac
    15181 aagctgatcg aaggctcgcc gcagaacgcc atcacggcgg tgccgatcga tcacgccaca
    15241 ccgatcccgc tgcggccttg atgccgggtg cgccttcggt acctgccgta tccaggatga
    15301 ccgcgcggcg caccgcggac gcatcggtca tgcggcagcg ccggtgatcc caggcgcggc
    15361 ctgaagcgcg tggtgtggcg gtgggcggcc ctgtcggacg ccggggtggc ggaagggcat
    15421 gggagtcgaa cccacccggc ggcgcttggc gccacccacc gggtttgaag cccggcccac
    15481 ccaccaggat ggttgccctt ccgttgacct acgaaaacag cgcgcagtcc gtgcgcaatc
    15541 ggtgctcgtc gccgatgcgg cgcgtgaaac cgatgcggtc gaagtattcc agaatctgaa
    15601 tcgcccgctt gcggcccagg tcggtcgcgt cgcggaagtc ggccgcccgc acgatgccct
    15661 ggtgtgcggc gcccacctgg cgcgccaggg ccgccagccg ctgcagggtg tcgggcgggt
    15721 aatacaggtc cttgacgatc tggtgcagct cgccgcgccg cgccagccgc gccagcgtgg
    15781 tgcgcaccag cggctcgggc tcggcggcgt cgcgcgccag atcgcgcgcc caggcgccct
    15841 gaaagccggc ctgcaccagc gccggcgcca ccttctgtgc aatgcgcgct tcggtggccg
    15901 acagctgcgc gccgtggctg ggcaggtgca ccaccgcccc gtgcagcacc agttgccctt
    15961 cggtcgccag acgcgccatc aagccctgcc acaagggggc tggcaggcgc ggtgcactca
    16021 ggcggcgcag gcgggcgctg tcggggccca gctcgtcggg ctgctggtcg tgaaaacgct
    16081 gcagcgccgc cagcgcagcg gccgccagcg tgtgcgcctg gtcggcgccc agggccaggc
    16141 cgtcctgcac cagcgccggc gccggcagcg cctggtcggg cgggtgggcc aggccctggg
    16201 cggcggcaaa gcgcttcaga tcgacgccgt gcggcgccac cgccagcagc gccgtcaggc
    16261 gctcggcagc gctcggcaga cccagggcgc tcagctcggc cagccgctgc ggcgtgcgcc
    16321 ggtagcgggc gggggccagg gggtcgagca cgcgaccgcc cgccagcgtg cggctggctg
    16381 ccgcatcgcg cagcagcacg cgttcgccat gccaggcgcc caccggggcc tgcagcagca
    16441 gctgcgccag ggcggtggcg ccgggcgcca gtgcgtcgct gtccagcagg gccacggtgg
    16501 cgttgacgtg ggccgcgccc aggtgggcct gcacccgggt gcccgagcgc agcgcctggg
    16561 cttcgcccgg ccacagcgtc aggcgcaggt cgaggcgctc gctggccagc gccacggcgg
    16621 gggcggtcag ccattgaccg cgcgtcacct cgtcgcgcgc cagcccggcc agggccacgg
    16681 cacagcgctg gccggcgtgg gcgcgtgcca ccgcctggtt ctgcgcgtgc aggctgcgca
    16741 cacgggcgcg caaggtgcca ggcacgatcg ccagctcgtc gcccatcccc accgagccgg
    16801 cgtggatggt gccggtgacc accgtgccgg tgccctgcag ggtgaaggcg cggtcgatcg
    16861 tcaggcgcag cgctttggag tcaaattcgg cctcggagct tgcctgttct gcgccagcag
    16921 ctacaaaaag aagagctcgc aggtcgtcga tgccggcccc ggtgtgggcc gacacggcca
    16981 gcaccggggc gctttcgagc gtcgtgccgg ccagcagcgc gcgcgtctgc tcgcgcaccg
    17041 cctgcacgcg ctcgggcgtg gcgcggtcgg ccttggtgat gacgacggcg ccgcgcgtca
    17101 ggcccagcag gctgagcacg gccaggtgtt cacgcgtctg cggcatgggg ccgtcgtcgg
    17161 ccgccaccag cagcagcgcg aagtcgatgc cgctggcgcc ggccaccatg gtgtgcacca
    17221 gccgctcgtg gcctggcacg tcgatgaagc cgatgcgcgc gccgctcggt ggttcgagga
    17281 aggcgtagcc cagctcgatg ctgatgccgc gctttttttc ttcgggcagg cggtcggtgt
    17341 ccacgccggt gagcgcgcgc accagcgtgg tcttgccgtg gtcgatgtga ccggcggtgc
    17401 cgacgatcat gagctcgccg cagtcaggtc cggaaggccg ggttgggccg caccggcggc
    17461 gctatgccgc caaacatctc tccgaacatg aagtccagcc agcggaagtc gtcctgaaac
    17521 agggccggcg ttcgttcgtt gaagtcgcgc tgtacttggc gcacgcgacc ttcagggtcg
    17581 aagtcgtagt cgaactgaac gttgagcgcc tgccccggcg tgccattgac caccatgtag
    17641 cacaggttgt cgggcaggcg ccgctcgggc gcgcgacctg ccagtcgttc gctgatctgg
    17701 taggcgacga tgcgacccag gcggttggca atgtgggcac tcttggggta gtagcggaag
    17761 ggcagagacg aaaccgcctg gatggcgtca ccgatgacga aggtgcgctc gtcgccccgg
    17821 acctgcatgg tcacctgatc gacgtcggcc cagccctcgc tgcggtcgct gacggtgcct
    17881 gccagccagg cgaggtcgcc ggccttgtgc ggtgccatca ggatggcatg gtcgaagcgc
    17941 tgatcaccgg cgcgggtacg gatgcggcgg ttgaacgggt ccacctcctg cacgtgtgcg
    18001 ttgggcacat aggtgatgta gtccttgtac agctcgtcga acgcggctcg gtagccgggt
    18061 ccgatggggg tgatggcggg cttgtggtcg aggatggtga tgtggccctt gatcttgttc
    18121 gtcttgaagt accaggcgat caggcaggcg cgctcgtagg ggctgggtgg gcagcgttgc
    18181 gggggaggcg gcaggttcat caccagctcg ccccctttga agtcctgcag cgatcgcttg
    18241 atggccagga attcgctgtt gctggcatag gcgcagggaa agcgcgtgcg tgcggcgtcg
    18301 gccgccacgc ggtcgttgcc gaaccaggct tcgtactggt aacggatgcc cggtgccagc
    18361 accagccagt cgtagtcgat cgtccccagg ctggtggtca cgcgacgctg cgcgcggtcg
    18421 atggcctgaa cctcggcctg cacgaatcga tagcccagct gctccgacac acgcaggtag
    18481 tcctgcgtga gcagttcgcc atccaccatg ccgaccagcc acatgttcga caacgggctg
    18541 gagaagaatt gcgggttgcg atcgatcaaa accacttctg cgcctggcgc ctgcttgcgc
    18601 aggttggccg ccgtggtcag accgccccag ccgccgccca cgaccagcac gcgcggcgcc
    18661 ttgcctctgg gaagcaaggt ctgggtcgac tggccgatga acgcgggcat cggcgcggtt
    18721 tgaacgccaa cgccggatac cggaggtgcg gcggcacagc ccgccagtgt ggcggcgcca
    18781 gccaggccgg cgacgaagtg gcgacgatcg atggtcatgg gtttacttgg ttgcaggtgg
    18841 aacggtcgtt gcttgcggag acaactgccg cagctggcgg gtcagcaggg tgctgtcttc
    18901 caggcagcgg caatccagaa tgagctggtc gtcggcaatg cggccgatga ctggctccgg
    18961 aagccgacgc agggccgtgg caagctcgtc cagtgcgcga ccgcggccgg tcctggccac
    19021 cggcgcgatg gccaggcctg ccgagggcag gcgctcgacc ggcagcgagc cggagccgat
    19081 ctgtccttgc agcgccaccg tctcgaccgt gaagcgcggc gccagcgccg ctgccagcgc
    19141 cggctgcacc gcgcgggcgg tggcttcgat gtcggccagc gggcgcgtga gcaggcgcag
    19201 cgtgggcagc tcgcgcgcca gccgctcggg gcgcaggtac aggcgcagcg tggcttccag
    19261 ggcggccagc ggcagcttgc tcatgcgcag ggcgcgcttc atggggtgct tgcgcacgcg
    19321 ctcgaccagc gcccggcggc cgacgatcag gccggcctgc gggccgccca gcagcttgtc
    19381 gccgctgaag ctgaccagat cgcagccggc gcgcagcatc tgctgcggcg tcggctcggc
    19441 cggcagaccg taggccgtca tgtcgatcag ggcgccgctg cccaggtcgg tcaccagggg
    19501 caggccgtgg gcgtgcgcga tcggggccag cgtggcttcg tcgaccgcgc gggtgaagcc
    19561 ctgcaccgca tagttgctgg tgtgcacctt catcagcaga gcggtgcgcg cggtgatggc
    19621 gcgctcgtag tcgtgcgggt gggtgcggtt ggtggtgccg acctccacca gccgcgcgcc
    19681 ggcgctggtc atgacgtcgg gcatgcggaa ggcgccgccg atctccacca gttcaccgcg
    19741 cgagacgatc acctgccgcc cgcgcgccag cgcggccagg atcagcagca ccgcggcggc
    19801 gttgttgttg accacggtgg cggcctcggc gccagtcagc tggcacaaca gaccctcgac
    19861 gatgctgtcg cggtcgcccc ggccgccgct ggccaggtcg tattcgaggt tgttgggccc
    19921 ggccatcgtg gcctgcacgt gggccagggc ggccgccggc agcagggcgc ggccgaggtt
    19981 ggtgtggatc accgtgccgg tcaggttgat cacggtgttc aggcgcggcg ccaggcggtc
    20041 gtgcaggcgg cgcgccaggg cgcccgccag ggcgtcgggg gcgatttcgg cggccggcag
    20101 ccggccggcc agggcctgct ggcgcagttc ggccagcagg gcgcgtgcct cgtgcgtgag
    20161 caggctgcgg ccatgttcgg cctccagcgc agcaaccgcc ggcaggcgca gcaggcggtc
    20221 gagggcgggc agagcgtggg gggcggaggt ggtcggggcg tctggcgggg ccggcgtacc
    20281 gtgaaccacg gattgctcag gcgccgtcat cagcgctccc cgggcggcgc ctcggcgtca
    20341 ccgaacagca gcagcaggtt ggtgccgtgg cgcgtgaagc cggcatcgga caccagcaga
    20401 tcgagcgtca gtgtggccag gtcgtcggcc accgggtcgg cgtgcggctc gcgctccatg
    20461 tgcagcagct tcaggtagtg gtgacagtga tcgcaggttt cggcttcgac cgcagcccgt
    20521 tctgacgtcg gtgcggccgc ggccagcgcc tgcagcgcct ggtagtggat gccctgggtg
    20581 cccaggcagt gcgtgcacat gacctgcacc atgtgccatt cggtctggca cagggtgcag
    20641 tgcaggtagc gctgcccctt catgcccggg ccgacgcgcc ggatgctgct ggtgggcagg
    20701 ctgccgcagc aggggcaggt gccgcggctc tccaggtgtc gccacagccc cgggtgggcg
    20761 ctcgcggtgc ctgccaccag atgcgcccag tacagctgca ggccggcggc gatcagcggc
    20821 gccgccgcca tgtccagccc cagcgtgatg ccggccagca ggcgatcggc ctgttgctcc
    20881 agcgcctgat ccggcaggcc gatcacggcc tggaccgtgg cgcgggccgg gctgtcgccc
    20941 ggcagctgct gcagcacctg ggccagcagg gcgcgcagct cggtgcgcca cagcggctcg
    21001 cgcggccagg cggtggccgc cagcagcggc tggccggtgg cggcgctggc atcgatctgc
    21061 gccgcgtcgg gcagccttac ctgcgggtag cggcgcgcgc gctcgtgctg cgcctcgctc
    21121 accaacgcga gcaacagcag gtagtcgcgc atggcatggc cggcggccag ctcgcgcagg
    21181 cgcagcgccc gttcggcaaa cagcgtgtcg gcctgcggcg cgcgcacggc ggctatgtcg
    21241 gcgccggcct gcagagcgat ctcttcgggc gagggcagag tggggggatg ggtggtggtc
    21301 aaggcaaaaa agaactgccg ccggcgcacc gcaccggcag gcagtggggg tgggtggggg
    21361 aaatcaggac aacctcattt gacgcgcgcg tcggacgatg ggttctgcat gcgttcacgg
    21421 taccacagcg gatggtggtg ccgagcccag ctggcgctga cgctgccgcg cgtcatggcg
    21481 cgcagcgtgc ccttgaccca gaaggcggcg taggcgtgca cgatcacggc cagcaccagc
    21541 accacggcgg cgacggcgtg cagcacgatc gccatgcgtt gcatgccgat cgatgcgccg
    21601 ccgccgaacc actgcggcca gaccaccacc agaccgctga tcagcagcac cagcaggctc
    21661 agcgcgaaca gccagaacac cagcttctgc ccgccgttgt atttgcccac cggaggcatg
    21721 tcggcgtgtc cgccctcgga agacaccagt ttgccggccg atcccagcca ttgcgagtct
    21781 tcgctgcgcc agacgttggc gccggccagc atgaagaaca tgcccagaaa cagcaggcac
    21841 atggcggcac ccaggaaggg gtgcaggatg cgcgtccact gcgggccgcc gaccagggcg
    21901 ctcagccaga acagcgccgg atggaacagc gcgaagccgg acacggcggc cagcacgaag
    21961 caggccacca ccgcccagtg gttggatcgc tggccggcgc tgtagcgttg caggtacatc
    22021 ggcttcatga cgactctcct tccgtgcggg tcgcgccacc cgcggtggct tcagcgtcct
    22081 cggggacttc cagcgggccc ttgcgcaggt agtggaacca gcccgccagg gcggcgccgg
    22141 ccatcgcggc catggcgacc ggcttggtcc agcccttcca gaccgacacc cacgggctga
    22201 tgctcggctc cttgggcagg ccggcgagct cgggccggtc ggcgtgcttg agcacgtaca
    22261 tgacgtgcgt gccgccgaca cccggcgggt tgtacagccc ggcgttctga tggccgcgct
    22321 ccttcaggtc ggcggcgcgc tgctggccgt agctcaccat gtcgtccttg gtgccgaagc
    22381 tgatggcgcc ggtcgggcag gtcttgacgc aagccggctc cagccccacg ccgacgcggt
    22441 ccgagcacag ggtgcacttg taggccttgt tgtcctgcgg gctgatgcgt ggcacgtcga
    22501 acgggcagcc cttgatgcag tagccgcagc cgatgcagtg ctcgctgatg aagtcgacga
    22561 tgccgttggc gtactttacg atcgcgcccg gtgacgggca ggccttcagg cagcccgggt
    22621 cctcgcagtg catgcagccg tccttgcgga tcagccattc gagcttgtcc tgctcgggct
    22681 cggcctcgta gaagcgcatc accgtccagg actgggcggt gaggttggtc gggttgtcgt
    22741 acaccccgat gttgctgccc acctcgtcgc gcaggtcgtt ccactccatg cacgccacct
    22801 ggcaggcctt gcagccgatg cacttggaga cgtcgatgag cttggcgacc tgcggcgtgc
    22861 tgctgcgcgc ccgaggcggg ggggtcgtcg tggccgagcg ggcgaccacg tcctgggatt
    22921 gcaggctgga catggcttac accttttcga tgttgacgag gaaggccttg aactccggcg
    22981 tctgcgagtt ggcgtcgccc acgaacggag acagggtgtt ggccaggtag ccgttcttcg
    23041 tctgaccctt gaaaccccag tggaagggga tgcccaccag gtgcaccttc tggtcgttca
    23101 cgttccaggg tttgatgcgc ttggtgacca ccgcccgcgt cacgatgtgg ccgcggttgc
    23161 tgctgacctt gacctggtcg ccgtgccgga tgttcttttc cttcgccagc tcttcgctga
    23221 tttcgacgaa cgattcgggc tgcacgatgg cgttgagccg cgcgtgcttg gtccaggtgt
    23281 ggaaatgttc ggtcagccgg taggtggtgg ccgagtacgg gaactcctcc ttcttgccaa
    23341 aggcctccag gtcgcccttg aacacccgcg ccgccggatt gctgatggcc ttggggttgt
    23401 cggggtgcag ggggttgacg ccgatcgggg tttcgaacgg ttcgtagtgc tccgggaacg
    23461 ggccgtcggc catgccgatg gcgtgcagcc gggccacgcc ctcggggttc atgatgaacg
    23521 aaccgacgtt ctcctcgggt gcggcgttgg gccgcatgtc ggggacgtcg gcgccggccc
    23581 aggccttgcc gttccagtac acgtacttct tgttctccga ccacggcttg ccgttcttgt
    23641 cggcgctggc gcggttgtac aggatgcggc ggttggccgg ccaggaaaag ccccagttct
    23701 ggtacacgcc cagaccggtg gggtcggata cgtcgcgccg cgcggtcagg ttgccggctt
    23761 cgctccagca gccggcgtac agccagttgc cgcaggcggt ggaaccatcg tcgcgcagca
    23821 tggcaaagcc cggcacctgc tgacccgcct tcaccagcac ctggcgcggc gccgtggggt
    23881 tgttggggtc gggcggtgcc agcacgtcgg acagcgcttt gccgttgatt tcgcgcagca
    23941 cttcggtggc cgatggggcc tcgggctgca cgtagtccca ggccagaccg gcgatggcgt
    24001 cggggaaggc gccgccgtcc ttggcgtaca tctgacgcag cttgaggaac agccgcgcca
    24061 tgatctccga atccaccttg gcttcgccgg ggggttcgag cgcgcgctcc ttccagatcg
    24121 cgatgcggct ggagttggtg aagctgcctt cctcttcggc aaagcaggcc gaaggcagac
    24181 ggaacacctc ggtctggatg ctttcgggct tgacgtcgtt gaaggggccg tggttctgcc
    24241 agaactcacc ggtttcggtt tccagcggat cgatgatgac caggtacttc agcttggaca
    24301 gcgccgccga cagcttgttc ttgttgggca ccgtcgccag ggggttgaac ccctgcgaga
    24361 tgaagccggt catcttgccc tggtgcatca gctcgaacat gtgcagcgca tcgaagccac
    24421 cggccagctt gggcacccag tcgtagcagt actggttgtc cgccgtggcc gccttgccgt
    24481 agtaggactt catcaggctg atgtgaaagc gcgggaagtt gctccagtag ttgaactggt
    24541 tcggccgcat cggcttgggc gtgcgcttgg ccaggtactt ttcgaaggtg tcgtcggcgt
    24601 cggtgggcgc actcaggtag cccgacagca cctcggcgta ggcgttcatg tcggtcacgc
    24661 cctgcacgtt ggagtgtccg cgcagcgcgt tcacgccgcc gccgggccgc cccatgttgc
    24721 ccagcagcag ctggatcatg gccatggtgc ggatgttctg gctgcccacg ctgtgctgtg
    24781 tccagccgag cgcgtagagg atggtgctgg tcttgtcggg cgcggacatc tcgcccagca
    24841 tttcgcacac cttcaggaac ttgtcctgcg gcgtgccgca gatgcggctg accatctccg
    24901 gcgtgtagcg gctgtagtgc ttcttgagct gctggtagac gcacagcggg tcctgcaggc
    24961 tgtcgtcgac cttgacgtgg ccgttctcgt cgagctggta gccccagctg gccttgtcgt
    25021 acttgcgctt ggcctcgtcg tagccgctga agatgccgtt ttccagcttg aagtcgggct
    25081 tgagcaggaa ggtggcgtcg gtgttgaact tgacgtagtc gtgatggatc ttgtcgtggc
    25141 tcagcagcca gttgatcacg ccacccagga aggcgatgtc cgtgccgttg cggatcggcg
    25201 cgtacaggtc ggccaccgag gaggtgcggt tgaagcgcgg gtcgaccgag atcagccttg
    25261 ccttgcgctt ttccatggca tccaccgccc atcggaagcc gacggggtgg gcttcggcgg
    25321 cattgccgcc catgacgaga atgagatccg tattgcggat gtcagtccac gaattggtca
    25381 tcgctccgcg accgaacgtc gggcccagac tggtcaccgt cggtgcgtgt cagacgcgtg
    25441 cttgggtatc tatccccacc atccccaagg cacgtgccac cttgacggac agatagctgg
    25501 attcattgga cgacgaactg cacaccagaa aaccggtggt gggccagcgg ttgaccgtgg
    25561 tgccggcgtc gttcttggcc tggaagttgg cgtcgcggtc ggccttcatg tgcctggcca
    25621 cgcgggtcag cgcctcgtcc cagctgatgc gcttccattc cttgctgcca gcctcgcgca
    25681 ccgacgggta gatgacgcgc ccgggcgagt tgatcatgtc catgacgccg gcgcccttgg
    25741 gacacagcgt gccgcggttg accgggttgt cgggatcgcc ttccacgtgg atcacgtgtt
    25801 ccctgacgtt cttggcggtg tcgcccagct tgtagatcag catgccgcaa ctgaccgagc
    25861 aatacgggca ggtgctgcgc gacacgctgg cacgggtgag tttgaaggcg cgtgtctcgg
    25921 ccagggccgc ggtgggtgaa aaacccagcg cgaccaggct ggacgcggcc aagccagctc
    25981 cgctgacgcg gaagaactgg cgccggttca tgtccatggg gcggtctcct ctcgtgggat
    26041 gctttgcccg tgtcgacatg cacgccgacc gggatgcacc agccctcagt ctagggaggg
    26101 tcagggttgg tgccactgag gtaaacccta tgtaaggagt gttgcagcgc cgcccggcac
    26161 acgccgggcg tgcgcggcct gccgccgcag agggttcagt cgcccatcac cacgccttca
    26221 cgccgtggat cggcgccgcc cagccaggtc ggttggccat gcacggtgcc gcgggtgatg
    26281 gcctgcaggc cgctggtcag gttcatctcg cgcacttcgg cgccgcgcga gcgcagggct
    26341 tcgacggtgg cggcggggaa cttcttttcc tccagcaggc tggggccgtt gagcgaaccg
    26401 aagttgggca ggttgatggc ctgctgcggc agcaggcccc agcccagcac gccgtgcagg
    26461 gtcttggcgg tgtagtggat gatgagggcg ccgcccgggc tgccggcgct catcaccagc
    26521 gggccgcggc ggccgtcggt ggtcttttcg aacaccaggg tgggcgccat cgacgagcgc
    26581 ggccgcttgc cgggctgcac gcggttggcg atcggctggc cctgggcgtc ggtcggggcg
    26641 aagctgaagt cggtcagttc gttgttcagc agaaagccct tgaccatctg acgggcgcca
    26701 aactggtcct cgatggtggt ggtcatggcc agcgcgttgc cgaaggggtc gacgatgctg
    26761 atgtggctgg tgccgtattc gggctggctg gccatggggg cgaagctggt tcgggtgccg
    26821 acgggcttgc ccggcggcgc caccttcagg ctctgcgtgc cgatcagcgc agcgcggctc
    26881 ttcaggtagg ccggatccag caggctcatc cagctgccgg cgggcggctg cacgaagtcg
    26941 gggtcggcca cgtacagcgc acggtcggca aacgccagcc gcgaggcttc catgtagtag
    27001 tgcagccaat cggccgtggg caacccgtcg gcgccggccg ccatgctggc cgccggggtg
    27061 tgggccagga tgcccaggat ctggccgacc gcgatggcgc ccgacgacgg cggcggaaag
    27121 ccgcacacct cataggcctt gccgctggcc ggcacgcgca gatcgtggca cagcggcgcg
    27181 cgccgcttgg gggcgtagcc ggccaggtcg gccagcgtca gcttgccggg gttggtgggg
    27241 tgcttctgca ccttgtcgac gatggcctgc gccaccgggc cttccagcag ggcggtcgag
    27301 ccgcgcgcgg caatgccctt gagcaccgcc gccagttcgg ggttcttcag cacatgcccg
    27361 accggccagg ggttgccggc ggcgtcgtag aagtggcggg cggccaccgg gtcctttttc
    27421 aggtgctgct cgttcttcag cagggtgtgc aggcgcgggc tgacggcaaa accctgctcg
    27481 gccagcacga tggcgggctt gaacagctcg gcccaggcca gacggccatg ctgacggtgt
    27541 gcctgctcga gcatgcgcac cgtgcccggt acgccgaccg agcggccgcc gaccacgccg
    27601 tcatggaacg gcaggggctt gccatcggcg cccaggaaca gcttgtcgtc gacggcggcc
    27661 ggcgccgtct cgcgcccgtc ccaggccgtg acgtcacccc cggccgcgtg cagcaggaag
    27721 gcgccgccgc cgatgccgct ggactgcggc tcgaccaggg tcagcaccat ctgcaccgcg
    27781 atggcggcat cgagcgcgct gccgccggcc ttgagcacct ggtagccggc gtcggtggcc
    27841 agcgggttgg cggcggcgac ggcgaacttg tgcgtcgccc agcccggctt gtcggtccag
    27901 cccgagccgc cttcgggggt gaccggtgtc gccggcggcg tgtagttcaa gctgctggcg
    27961 cagccggcca gcagggtggc ggcggccagg gcggccaggg tggtggggcg gtggggcaag
    28021 gccatgcgag tctcctggtg ggtggggtga gacggcatgg taacgcacgg caggtgctgc
    28081 gccggcctgg cgtgattcct gctcaaacag gcatgcggcg cttgtctggc aagcgccgaa
    28141 agctatcaaa acaatagcgc tagggatgcc cggcggtggc caccgcgcag ccggccgggc
    28201 ggagtggcag ggcttacttg ggtgccagcc ggatggcacc gtccagccgg atcacctcgc
    28261 cgttcagcat gtcgttctcg atgatgtggc aggccagctt ggcgtagtcc tcgggggtgc
    28321 ccaggcggct ggggaagggc acgccggcgg ccagcgcgtc ctgcacctcc ttgggcatgc
    28381 cgaacagcat gggggtgccg aagatgccgg gcgcgatggt catgttgcgg atgccgctct
    28441 tggccaggtc gcgcgcgatc ggcagcgtca tgcccaccac gccgcccttg ctggccgaat
    28501 aggccgcctg gccgatctgg ccgtcgtagg cggccacgct ggcggtgctg atcagcacgc
    28561 cgcgctcgcc ggtgggctcg ggctcgttgc ggctcatggc gtcggccgcc agacggatca
    28621 tgttgaaggt gcccaccagg ttgaccatga tggtgcgggt gtagccgtcc agccggtgcg
    28681 ggccgttctt gcctacggtg cgctcggcgg tggcgatgcc ggcgcagttg accagaccca
    28741 tcagcttgcc catcgacacc gccttggcca ccacggcctg gccgtcggct tcgctgctga
    28801 cgtcgcactt gacgaaggcg ccgccgatct cgcgcgccac cgcctcgccc ttgtccacct
    28861 gcatgtcggc gatgaccacc ttgccaccgc gcgccgccag catgcgcgcc gtgccttcgc
    28921 ccagaccgga cgcgccgccg gtgacgatga ataccttgtc cttgatctgc atgggagttg
    28981 ctccagatga gggaaacgcc aatggtaccc agcaatcccg cgcgcatcgt tgcaaataag
    29041 aattattcgc atatactgac ctcgccctcc cgctccaccc cttcaccgag gccaccctat
    29101 gtgcattcac tgctcccccc acgaccgccg cgacgacgac gagctgcggc ccaccgccga
    29161 ggccttgctg gccgccaccc tggcgctgat gacgggcgtg gccgaggctg cacccggcgc
    29221 cgccggcgtc cggccgatga gcctgaaggt gctggccaac ctggatgaac tggcgcgcca
    29281 tcccggcctg agtcggtcca tgcgccagct ggccgcgcac ctcggcctgc attggcagca
    29341 gctggtgcag accgcgcgtg gcgaagcccg cgccgccggc gagcgggcgc gctgggtgcc
    29401 ggcatcgacg gtggtgcaat gagtgcgctg gagccaggcg cgctgcagcg cgagcaccag
    29461 ctgctgctgc gccatctggg ccgggtgcag cagcgcgtga gcgagcagat gcttgcgcag
    29521 gcgcagcgct gtgcggcgct ggaggccgag ctgatgcaat ggcgggcacg ctggatcgtc
    29581 gccaccacgc gccgcctgtg ggggctgggc ggcttcgatc agccgccggc gccagcccgt
    29641 gccgtgccgg ccgaccgctc ggcctgggcc agcgccaccg aggtgctgtg ccagaccggt
    29701 tgcgccggcc atgcccatcc gtggcgcgac gagcaggggc gatgcgcgct caccgccagc
    29761 gactgcacgc gtgtgccgcc ggtcgcgcag gacgcttcag ccggcgccgt cgcccgcgtg
    29821 ttctcgcgcc cctgaggcca cccccagcag ccgctgcagg cgcgcgctgc tggtggtgta
    29881 ttcgaacggc agggggtcgt gcgggtggat gtgctcccgc gccgcgtagg cggccagcgt
    29941 ggcttcgtga aagccgcaca ccatgagttt cttcttgccg gggtaggtgt tgatgtcacc
    30001 gatggcgaag atgcccggct cgctggtctg gaaggtggcc gcatccacca ccagctgctt
    30061 gcgctccagc gccagttgcc aggcggtgat cggaccgagc cgcggactca cgccctgctg
    30121 caccagcagc agctcgagcg gcagcggctg cgtgctgccg tccggcccca tcacctgcaa
    30181 cgcgctcagg cgccccgcgg cctgctcgaa gcccaccacc tggcctaccc gaaagcgcat
    30241 cgagccggcc gcgcacaacg cgcgcacgcg cgcctgggtg gccggcgcgg cctgaaaacc
    30301 gtcgcgtcgg tgcagcaggg tgacgctggc gggcgcggcg gccgccagcg tggccagccg
    30361 gatggcctgc gtcagcgccg gctcgccgcc gccgacgacg accacgtgct ggccggccag
    30421 cgcggcgtct gccggtgcct ggtggaacag ctggcgtcgc tcgaacgctt ccagtccggc
    30481 gaccttcagg cgtcgcggca ggaaggcgcc cacaccggcg gcgatgaaca cgctgcgcgc
    30541 ccgcaggcgc cggccaggct cggtctgaag cagaaagcgc ccgtcgggca ggcgctgcag
    30601 cgactccacc aggtgaccga ggtgccactg gggcgcaaag ggcgcgatct gacgcagcag
    30661 cgcgtcggtc agcgtctgcc cgctgcacac cggcaccgcc gcgatgtcgt agatcggctt
    30721 gtcggcgtac aactcgaccg gctgaccgcc ggcatggggc agggcatcga tcacatgggc
    30781 gcgtagctcc agcaggccca gctggaacac cgcgaacagc cccaccggcc cggcgccgat
    30841 gatcgcgacg tcggtatcga gcgtgggggc agggcggtgc gggttcattg ggtgcagtgt
    30901 ttcgcgctgt caggtactta tagcgagtcg ccaaggggcc agctgcgcgg cgcgtggccg
    30961 caggcatggt ggagccatgt caaggccacg ccacaaagca gatggcccct tggcggctcg
    31021 ccctgcggga agccagaaaa aggcttgcgg cggcgttgcg cctcttgcca aggccgccag
    31081 catgggctgc gagccg
//
LOCUS       DAOQTZ010000019        29516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1853_length_29516_cov_14.337056, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000019 DAOQTZ010000000
VERSION     DAOQTZ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 29516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29516
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1853_length_29516_cov_14.337056"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKN51_03885"
     CDS             complement(<1..398)
                     /locus_tag="PKN51_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84270.1"
                     /translation="MDSEIRNADAVAAEPLNPACPELHRWGARQLYGRWSVELTELGQ
                     RGELVLRQHPEFLESLRGEFVIDGQISIASGDVDQGEFNLDESRDGKSLFAFWNGRLV
                     PAACGREIRGSWQSLPQPGVPARESSFVLRR"
     gene            complement(472..2523)
                     /locus_tag="PKN51_03890"
     CDS             complement(472..2523)
                     /locus_tag="PKN51_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNT84271.1"
                     /translation="MSDRLNLAQQDAVHHLRGPCLVLAGAGSGKTRVITHKIARLIAT
                     GVEPARIAAITFTNKAAAEMRERARGLVGKAAGEVLICTFHALGVRMLREDGATLGLK
                     PQFSILDSDDVLGLLKDCGATTDAATARQWQWVISGWKNAGLDAAQALAQARGEGERQ
                     TALLMARYQERLSAYQSVDFDDLIGLPLRLLQTHEHVAHKWQARLAHLLVDEYQDTNA
                     TQYELMKLLVGPQGSFTAVGDDDQSIYGWRGATLDNLRRLPQDYPRLKVIKLEQNYRS
                     TSAILRAANNVIGPNPKLFEKRLFSELGEGEPVRVLACDHEQHEAERAVARIQSLRQT
                     SAHKDWRDFAILYRANHQAKVFEQALRRAQIPYKVSGGQSFFDRAEIKDLCAWLRLWI
                     NEDDDPAFLRAVTTPRRGIGHQSLAALGEFAGKAHLSLFGALFAHSLPAALPARTIAS
                     LHEFGRLVNDLRHRAGHTHGHEAARTFLADWLKDIGYEQHLHDSADSAPQAAARWSNV
                     LEFCDWMAGRCGGQIEDAAGVTLHQESRSLLEVAQTISLISTLNEREQDPNVVTLSTL
                     HAAKGLEWPHVVLAGVNEGLLPFRPDEDAGAAAQAARIEEERRLMYVGITRARNTLAV
                     SWTRRRKKGREVVAAQPSRFIAEMALDAATVREDPRAKLKALRAEFAQRAASAPPQAA
                     P"
     gene            2571..3545
                     /locus_tag="PKN51_03895"
     CDS             2571..3545
                     /locus_tag="PKN51_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNT84272.1"
                     /translation="MHALAGIFSVTFPFFALVLCGYLAARSRLLPLDAVPGLNMFVLY
                     FALPCMLWRFGSGTPLLQLFHPLIALLWLAAAALIVAGTAWLARRRGGGWPDAAMGAL
                     VAAFPNSGFMGVPLILALLGEAAVGPVMATLLVDLVVTTSVCIGLSQWGAADEHGAAK
                     AAGRALQGVLRNPMPWSILLGALTGALGLAPWAPLARTVDLLADAASPVALFTIGAVL
                     ARSQVQAQTRAQSGAAGLGDVGVITLVKLLVHPLLVWALGWLAVRAGGLTPATVTSLV
                     LTAALPAAGNVSLLAERFGADNGRVARIILWSTALAFFSFSGVVALRP"
     gene            complement(3511..4422)
                     /locus_tag="PKN51_03900"
     CDS             complement(3511..4422)
                     /locus_tag="PKN51_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNT84273.1"
                     /translation="MTPPPHPRRRAWLCRAGLALVLSAGGLPAGAWNARGHAAIAALA
                     EAGLNDAARAQVQALLAQDLDRFGRPSGRRTLAAVASWPDEIRAEAARRDPEAYRGWH
                     VRANPVCGERLGRCPQGHCVDQLIIHHTAVLRDHTQPLRQRNEALKWVVHLVGDLHQP
                     LHSGVNPNGGRARVELVGATPPADASLHAVWDGALLDAALAGWQPRTPAADTAALALD
                     APTGWMLETRDVALTQVYQPLNGFACGIRLAEPLRLDADYQRASVPVIRQQIERAARR
                     LTQLLHQALAAQGAEALTDAAPPPPKN"
     gene            complement(4470..6590)
                     /gene="priA"
                     /locus_tag="PKN51_03905"
     CDS             complement(4470..6590)
                     /gene="priA"
                     /locus_tag="PKN51_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNT84274.1"
                     /translation="MTHWLAVAVATPAHSGLGEPLTYRADAPLAPGSIVRVPLGAREV
                     LGVAWGTAATPAPDWAAQVRPLAGVFEGLTPLSDDWRRLIAFTASYYQRALGEVALAA
                     LPPQLRTLSSAQMARRLRRLGEAPPAGAAAAAAPTALTAEQAAALARIAQEPGPFLLH
                     GATGSGKTEVYMQAAARVLAHQPAAQVLVLVPEINLTPQLEARFAARFGAAACVSLHS
                     GLTPPQRLAAWLAAHSGRARIVLGTRVAVLASMPQLALIVVDEEHDPSYKQQEGARYS
                     ARDLALMRGRLAGAQVILGSATPSLESWHASAPAPEGPGRLRRLAMPRRVGLTPADDP
                     ATGLPRVRRVDMSRQPRGTLIAAPLLAAIGERVARGEQALLLLNRRGWAPVLHCADCG
                     WKSECPHCSAYRVFHRIDRSLRCHHCGFTQPVPRACPACGNLDLGSLGRGTEQLEAQL
                     AGLLADVRRPDGAPARIARIDADSTRGKGQLDSALAQVHAGDIDVLVGTQMIAKGHDF
                     RRVTLVAAIQPDAALFSSDFRAPERLFALLLQAAGRAGRDAASAAGSEMWVQTHHPQH
                     PLFEALKAHDYPGFAARQLAERAQAGLPPFSRLALLRAEARTQAAAQAFLAATAQAAQ
                     QPAHPDPQLAEALAQVTWYPAVPLAVQRVADIERAQMLLESPSRAALQRVLAACPPLL
                     HQLRTVPAHRGIVRWAIDVDPLAL"
     gene            complement(6991..8181)
                     /gene="hemE"
                     /locus_tag="PKN51_03910"
     CDS             complement(6991..8181)
                     /gene="hemE"
                     /locus_tag="PKN51_03910"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNT84275.1"
                     /translation="MTSFAPLHNDTFLRACLGQATEHTPVWLMRQAGRYLPEYCATRA
                     RAGSFMGLATNVQYATEVTLQPLERYPLDAAILFSDILTVPDAMGLGLSFAQGEGPKF
                     AQVVRDEAAVARLAVPDMDRLRYVFDAVTSIRKALNGRVPLIGFSGSPFTLACYMVEG
                     GGSDDYRHVKTLMYARPDLMHRLLTVNADAVAQYLNAQIDAGAQAVMVFDSWGGVLAD
                     GAFQTFSLAYTARVLGQLKRTGADGQVVPRIVFTKGGGLWLSDMAALDCDVLGLDWTV
                     NLGTARRMLTQIAQNATTLIAAGACPASAGGQNDATSRHPKALQGNIDPNVLFAPPEA
                     IRAEVRRVLDSFGRPHSDPAVPGSTHIFNLGHGISQHTPPEHVAHLVDEVHRHSRLLR
                     ARSA"
     gene            8330..11020
                     /locus_tag="PKN51_03915"
     CDS             8330..11020
                     /locus_tag="PKN51_03915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNT84276.1"
                     /translation="MDQHYLTPLFDPRSIALFTGPQDAPEQQTPQARALLAHLQAQAF
                     KGQVVPLSVHTTGTLADLAQARADLAIIALPDEEIGPALELAGRIKVKAALVLSHGVD
                     ARRARELQRLARRHGVWLLGPNSQGFQRPGLQLNASVAGALAAPGQLALVSQSGALTA
                     SILDWARQSRVGFSTVVALGPHTGVTLADTLDFLAADRHTSGIVVYMEGIASARRFTS
                     ALRAAANAKPVVVLKSGRQAAGNVAAQTHSGAIVGSDDVFDAVLRRAGAVRVRSFVEL
                     FSAAKCLASRYRPVGRRLAIISNGGGPGVLAADWVSELGLTLGVLSPEAARELAPRLP
                     AQASLSDLIDLSEGATAEHFRAALEVAAKDRAVDGVLVLYSPKFGGDADAVAQELVQA
                     RPRLAKPLVACWMGDASVQTARHALAEAQIPSFRTPEAAVGAFGNIASFYQNQQMLQQ
                     TPPPLTGLAAPDVEGARLLIESVLTERRSVLTEMESKALLSAFHIPVTQTLLARSANE
                     AMMIATQIGFPVALKIDSPDISHKSDVGGVALNVMHATAVRDIWQDMMAAVRERQPDA
                     RINGITVQAMARKPRGRELYIGVVTDEPFGPVIAFGAGGTLIELYRDHAMELPPLNQF
                     LARRLMERSRVHETLGPWRRAPAVDMAALEQVLLRVSEMVCELPQLREMDINPIIVDE
                     HGVLAVDARIVIGPAAQAAPGRAGRYGHLSILPYPARFEQWWPLRGGGEYKVRPIHPD
                     DAQPLQDMMKRLTPESRYFRFVSNMAELPPHMLSRFTLIDYDREMALCAVVEEEVPTE
                     DGGKTTRERIIGVSRYITNPDQTSCEFSLVVDDGYAGQGLGTRLMLSIMDAAREKGLQ
                     EIIGLVLTHNNGMLKLMRSLGFEVKAYPEDPDFRLVTHPL"
     gene            complement(11088..12239)
                     /locus_tag="PKN51_03920"
     CDS             complement(11088..12239)
                     /locus_tag="PKN51_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004545284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT84277.1"
                     /translation="MKPAPRPLFSPDHHAFREQVRRFCEREIAPQHAAWEEAHGVPRD
                     VWRQAGDHGLLCCWLPEALGGPGADLLFDFIVSEELGRIGATGPGFPLHSIIVAPYLA
                     AHGRPELQQALLPAMVRGDKIAAIAMTEPGTGSDVAAIRTQARREGEHYVLTGQKTFI
                     TNGHNADLVVVACKTDPAQGAHGVSLLVVERDMPGFTRGRNLRKIGQHAQDTAELFFD
                     QVRVPASHLLGDEGQGFKYLMQKLAQERLLISVQCQARAEAVLDWTVAYVQERRAFRQ
                     RLADFQNTRFKLAQLAADIAAGRAFCDQLIAQHLAGGLDAVGAAAGKLWHSELLGRVT
                     DECLQLFGGYGYMAEYPIARAYADARIERIYAGTSEIMKEIVARDLLDR"
     gene            complement(12313..13377)
                     /locus_tag="PKN51_03925"
     CDS             complement(12313..13377)
                     /locus_tag="PKN51_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT84278.1"
                     /translation="MPPAATPDRPPRPAGQTPISLVQAVVQAYTQRRLDPAAALLKAQ
                     IAPSDLARPGTQVTALQFERLCAAAMQELDDEALGWFSRRLPWGSYGMLARASISAPT
                     LGVALARWCRHHGLLTDDVALTLERSGAQAQVQIVERRALGALREFALLSLLRNLHGL
                     ACWLVDSQIVLQGAQFPFAAPAHAELYARLFPGPVRFGATCAGLRFDAHYLQLPLRRD
                     GTALDQMLRRALPILVWPYRRDRLLSRRVRALLQQPQAAHTAETLAARLALSARSLHR
                     QLRAEGTSVQQLKDEARRAQACALLLRTDWPIARVARAAGFASDKSFARSFRHWTGHT
                     PQGWRLHGDAESNRAPAPVE"
     gene            13525..15237
                     /locus_tag="PKN51_03930"
     CDS             13525..15237
                     /locus_tag="PKN51_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT84279.1"
                     /translation="MSTALTESCARGAQTPPLIEQTIGAFFDAMVARFPEREALVVAH
                     QGRRYSYRQLQQDAHRLASALLRLGLKPGERIGIWSHNNAEWVLMQLATAKAGLVLVN
                     INPAYRTAEVEYALNKVGCRALVTMPRFKTSDYLGMLRELAPEWAHARPGEPLQSQTL
                     PQLQHAIWIDVAGEGAEEPGFMRFSELLASGDPQDPRLAQVARLLHPQDAINIQFTSG
                     TTGFPKGATLTHRNILNNGFFIGEAMKLTEQDRLCIPVPLYHCFGMVLGNLAVLTHGG
                     CIVYPNDAFDPLTVLQTVQDEKCTGLHGVPTMFIAELDHPRFKEFDLSSLRTGIMAGS
                     PCPIEVMKRVQSEMNMGEVTIAYGMTETSPVSCQSSTDTPLDKRVSTVGLVQPHLEIK
                     IIDPETGRTVPVGERGEFCTRGYSVMQGYWEDPQRTAEAIDAEGWMHTGDLATMDGEG
                     YVNIVGRIKDMVIRGGENVYPREIEEFLYRHPKVQDVQVVGVPDQKFGEELCAWIIVK
                     PGQQLTDAEVREFCKGKIAHYKVPRYLKFVPEFPMTVTGKIQKFKIREAMVAELGLVQ
                     DQTA"
     gene            15387..17009
                     /locus_tag="PKN51_03935"
     CDS             15387..17009
                     /locus_tag="PKN51_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNT84280.1"
                     /translation="MPAIDTKLNPRSADFQANAQAMRALVDDLRAQVRKVSEGGGEAA
                     RKKHMDRGKLLPRVRVQMLLDPGTPFLEIAPLAALHMYQDRNGQDEAPAAGMVAGIGR
                     VSGVDCMIVCNDATVKGGTYYPMTVKKHLRAQEIAQQNRLPCIYLVDSGGANLPNQDE
                     VFPDRDHFGRIFYNQAQMSAQGIAQIAVVMGSCTAGGAYVPAMSDESIIVRNQGTIFL
                     GGPPLVKAATGEEVSAEDLGGGDVHTRLSGVADHLAQNDMHALALARQVVAHLNRHKA
                     PAGDLRTPCPPRYAAEELYGVIPTDTRKPFDVREIIARIVDDSAFQEFKARFGTTLVC
                     GFAHIEGMPVGIVANNGILFSESAQKGAHFIELCCQRKIPLVFLQNITGFMVGRKYEN
                     EGIARHGAKMVTAVATANVPKFTVIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVM
                     GGEQAASVLATVKRDGIEAKGGSWSQDEEEAFKAPIRAQYEQQGHPYYATARLWDDGV
                     IDPADTRRVLALGLSAALNAPVEDVKFGVFRM"
     gene            17049..17360
                     /locus_tag="PKN51_03940"
     CDS             17049..17360
                     /locus_tag="PKN51_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNT84281.1"
                     /translation="MRTNIDIDDELLATAMRAGPYTTKKETVEAGLRLLARQAAYREI
                     LKWKGKLKWEGDESIDWTRPAADEPEEALEYSVQQPRARHTKTKAAASSGRKAARGAG
                     R"
     gene            17347..17748
                     /locus_tag="PKN51_03945"
     CDS             17347..17748
                     /locus_tag="PKN51_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNT84282.1"
                     /translation="MLVVDSSVWIDFFVDRPTAACDELDRLLADGHTRLVVPDLVLYE
                     VLRGFRDERALRQARLLLQTLSVESCGGADIALAAAEHYRLLRARGITVRSSVDVMIA
                     AFCIEQDYLLLHSDRDFDAIAAHRGLRVWVH"
     gene            17751..18266
                     /locus_tag="PKN51_03950"
     CDS             17751..18266
                     /locus_tag="PKN51_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNT84283.1"
                     /translation="MPAWHSHFSTLARYNAWATGRLLAGVAALPEDDYRRDVGLFFKS
                     IHGTLNHLLVGEHLLWFRRFAEGVSPRLALDDEAETDRTRLAERLRQGAARWQPLIAS
                     WPAERFDGELHYTTMRGQAVALPFAATLAHVFNHGTHHRGQITAALTALGQPCPELDL
                     VYLLQQESRQP"
     gene            18263..19045
                     /locus_tag="PKN51_03955"
     CDS             18263..19045
                     /locus_tag="PKN51_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT84284.1"
                     /translation="MTAALRIDVQDHVATVTLARPELRNAFNDAVIAELSEAFTTLGG
                     DARVRAIVLAAEGPAFCAGADLNWMRRMADYTREENLRDAGQLAFMLRTLYECPKPTI
                     ARVQGDVYAGGLGLVAACDMAVSVDSAHFCLSEVKLGLIPATISPYVIRAMGPRAAHR
                     WFLTAERFDAAEARRLGLVHEVVGAERLDATVAGLLQALTSASPNAVRACKQLVQDVA
                     ERDITRLLIERTVEGIADIRASDEGKEGVQSFLQKRKPAWLA"
     gene            19157..19900
                     /locus_tag="PKN51_03960"
     CDS             19157..19900
                     /locus_tag="PKN51_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNT84285.1"
                     /translation="MPDWLASFIQWLHGMGLHLGPDSAAALGQAAESASQAAARMDMP
                     QLLALAAALGWASGFRLYAVMFLTGAMGAAGWIALPAGLQLLQHPAVLAASGFMMLVE
                     FFADKVPWLDSLWDAVHSVIRIPAGALLAGSVFGADSATMAVVAGLLGGSLATTAFAT
                     KATTRAALNASPEPLSNGLASLFEDGLVVAVVWLATQYPLAFGIALAVMLLLSALLLV
                     WLARFLKLVVRKLRGFFGHQVPGPTDARV"
     gene            19923..21935
                     /locus_tag="PKN51_03965"
     CDS             19923..21935
                     /locus_tag="PKN51_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HNT84286.1"
                     /translation="MFTKILIANRGEIACRVAATARRLGVRTVAVYSDADAHAKHVLA
                     CDEAVHVGGSAPRDSYLQWQRIIDAARATGAQAIHPGYGFLSENEDFAQACQDAGLVF
                     IGPPASAINAMGLKAESKRLMEGAGVPLVPGYHGADQAPALLRREADRIGYPVLIKAS
                     AGGGGKGMRAVERAEDFDAALASCQREARNSFGDDAVLVEKYVQRPRHIEIQVFADTH
                     GHCVYLFERDCSVQRRHQKVLEEAPAPGMTPELRRRMGEAAVAAARAVGYVGAGTVEF
                     IVEQPGGYEQPEQMKFYFMEMNTRLQVEHPVTEAITGQDLVEWQLRVAAGEPLPLAQD
                     ELLIDGHAIEARICAENPDNHFLPATGTLHVYRKPPHAAFEFGAVRFDDGVREGDAIS
                     PFYDSMIAKLIVWGETRQQALAKLDAALAATHIAGLQTNVQFLRHVLASDSFARARLD
                     TALIERERPRLFQQDRLGRDLAVAAAVAQTVLAERAAETHDPFSRTDGFRSHGVATRR
                     VDLEYRGEAVVAKLRYLQDGLMLQIGDAEAQPLSLTALPDGRIDLRYGTARELVQVYP
                     LGEVEHVFGSRGAVQITELDLLSRAEEGAGEGGRLTAPMPGKVVSIAVRAGDKVSKGQ
                     ALAVMEAMKMEHTIAAPQDGVVDEVLYAPGDQVAEGAELLRLSHPG"
     gene            complement(22008..23486)
                     /locus_tag="PKN51_03970"
     CDS             complement(22008..23486)
                     /locus_tag="PKN51_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HNT84287.1"
                     /translation="MSALEPGAEIDGFLIGEMIHKGGMALIYETRYADGRAAPFPMVM
                     KVPRMTAGDGAETIVSFEVEHQMMQALDGPHVPYLVAAGDLSVLPYLVMEYVPGRTLD
                     HWLDQVLQGQPLPPHELARLGAAVARAAHDMHQQNAVHLDLKPANVIIRDAAEGAPAD
                     APLHAVLLDFGLSFHAHYPDLLAEELREAVGSPAYMSPEQVVGVRGDPRSDVFAIGVM
                     LYELATGELPFGAPSSKGGLRQRLWMDPKPPRARRPDLPEWLQEVILRCLEPVAAERY
                     PSAAHLAFDLAQPEQVQVTERGRRLKGTGFRTHFKRWLRAAGTQYQPSPLPTRVIDEV
                     PILMVAVPHHDVTDATLYSLREAVRRSLGIRPGARLAVVTVVAPGAASSTDASRSETT
                     QHRQMLTMLKTWAQGLPQEQHQISYHVLEDGDPADAILRFARANHVGMIVMGAATHGL
                     QLQRFVATVPIKVAMDAPCTVILVKQSMPFEQLGAVPASLSA"
     gene            complement(23483..24247)
                     /locus_tag="PKN51_03975"
     CDS             complement(23483..24247)
                     /locus_tag="PKN51_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT84288.1"
                     /translation="MRLALLSDLHANRQALDACLAHAQAAGAERLAFLGDLVGYGGDP
                     AYVVQRVMDLAAEGALVVKGNHDGYAADAAGDGKTLGQAGALWTQEQLSAAQRRFLAE
                     LPLTLHQPPLLLVHASAAAPERWHYVETPLLAALSLDAACEPPVGGPDVHYVFGGHVH
                     RQTLYYRGGTGKLMPFTPTAGVPVPVPRHRRWLATVGSVGQPRDGRTDAMYALFDDVA
                     QQLTFHRVPYDVPSAAGAIRATPLPPFFADRLEQGR"
     gene            24368..25276
                     /locus_tag="PKN51_03980"
     CDS             24368..25276
                     /locus_tag="PKN51_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNT84289.1"
                     /translation="MSLPTQVRLIDVGPRDGLQNEKQPVPAAVKIELVQRLQAAGLRE
                     IEVTSYVSPKWVPQMADNHQVMGGIARQPGVRYSVLTPNLKGFEAALADRPHEIVVFG
                     AASEAFSQKNINCSVAESIERFAPVVAAARAAGIAVRGAMSCIVGCPYEGDIAPEKVR
                     QLAGLMKGIGVERVDVADTIGVGTPRKVQRAIEATLAHYDIDHVSGHFHDTYGQALAN
                     TLAALELGVWNFQSSVAGLGGCPYARGATGNVASEDLVFMLHGMDIATGIDLDRLIDA
                     GQFISQHLGRPPNSRVATAVLNKRAG"
     gene            25311..25805
                     /locus_tag="PKN51_03985"
     CDS             25311..25805
                     /locus_tag="PKN51_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNT84290.1"
                     /translation="MNHPTSPALPDAMRRVAAVLAAHGHPHAPILLDDAARTAQQAAD
                     ALGVVVGQIAKSIIFRRRSDDAAVLVIAAGDRRVDEKKVAALVGPVGRADADFVKART
                     GFTIGGVSPVAHASPPVVLIDRELYRFDVIWAAAGHPHGVFRLAPADLERLTGAPVAD
                     VAQG"
     gene            25812..26099
                     /locus_tag="PKN51_03990"
     CDS             25812..26099
                     /locus_tag="PKN51_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNT84291.1"
                     /translation="MAPVPRDFDTLARQAQQALRDGTPLPSPCIGVCRIGDAGVCVGC
                     ARRLDEIGGWLHADEAGRGAIWRRVLQRRGLRAGAGGAAPDPARLAQGEQR"
     gene            26212..28032
                     /locus_tag="PKN51_03995"
     CDS             26212..28032
                     /locus_tag="PKN51_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HNT84292.1"
                     /translation="MPNAFNFNASPFDSLTPQEQRLVRDHVDVVYFRPDEVILDAGIA
                     PMHLFVIIKGVVQQFDGEELVATYGPDDSFDGRALVAGRASSRFVAVEEVVAYALARQ
                     TVNDLIAANATFGALVFSDLGAKLSAASQRHSGNELQALNMARVDEAFVRPAHLVEAD
                     TDVVSVVRLFQKERTTNVLVRDAASQPPRLGMFTTNALQRAILSGRPLEQIAVGELSN
                     YALISVRPSDLVGDALATMVRHHIHRVVVREGEQVLGVLEALDVFSFLSNHSMLITLR
                     INGARDIDELAEAAAQITDMIRRQFRGGTRAELIARLVQDLNARLFDRAWQLIAPAEL
                     VAHSCLFVMGSEGRGEQLLKTDQDNGLILRDGYAPPADLAELCQRFSDALARFGYPPC
                     PGGIMVSNPAWRMGATEFAQTTRRWLALPEGDSLMNLAIFMDAHAVSGDASLLDQVRD
                     SLTQMAVDSDAMLARFAGAIDAFGGSIGWWNRLLGGEAAQLLNLKKEGIFPLVHGVRS
                     LALAARLPVTGTVARIEALVQRGELSADTGRELTDSLHFFLSLRLKAGLAELDRQQRV
                     TGAVDFKSLNSLERDLLKDALDAVKRFKAQLRYRFRLNAL"
     gene            28038..28682
                     /locus_tag="PKN51_04000"
     CDS             28038..28682
                     /locus_tag="PKN51_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNT84293.1"
                     /translation="MSGLLERLRRGWQRRQLTDPRWRFLLDEPPPHEWVALDCETTGL
                     DTRTDEIVAIGAVRIVGQRILTSERLELLVRPEKGVSAESIRVHRLRQQDVQTGVPVA
                     EAMQRLLHFIGARPLVGYYLEFDVAMINRALRATLGLTLPQEKIEVSRLYYDHKFRQL
                     PPYRQHDQADIDLRFATIMRELGLPTRDAHDALNDAVMAALAFIKLRVLRGDAD"
     gene            complement(28788..>29516)
                     /gene="actP"
                     /locus_tag="PKN51_04005"
     CDS             complement(28788..>29516)
                     /gene="actP"
                     /locus_tag="PKN51_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059048.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNT84294.1"
                     /translation="IGFGAITLVLTNPEFADTAKGVIKGGAGTANMAAVLVAKSVGGD
                     VFYGFISAVAFATILAVVAGLTLSGASAVSHDLYATVFKKGKADSAAELRVSRITTLT
                     LGVIAVLLGIVFEKQNIAFMVSLAFAVAASANLPPLLLSVLWKDCTTRGAVIGGFLGL
                     ISSVVLTVVSPAVWEATLGNPKGSAWFPYSSPALFSMTIGFVGVWLFSVLDRSSNAAA
                     ERAAFAAQQVRSETGLGAAKASAH"
BASE COUNT         4345 a        10246 c        10568 g         4357 t
ORIGIN      
        1 cgccgcagca cgaagctgct ctcgcgggcg ggcacgccgg gctggggcag ggattgccag
       61 ctgccgcgga tttcgcggcc gcaggcggcg ggcaccaggc ggccgttcca gaaggcgaac
      121 aggcttttac cgtcgcgcga ctcgtccagg ttgaactcgc cctggtccac atcgcccgag
      181 gcgatcgaga tctggccgtc gatgacgaac tcgccgcgca ggctttccag aaattcgggg
      241 tgctggcgca acaccagctc gccgcgctgg cccagctcgg tcagttccac gctccagcgg
      301 ccataaagct ggcgcgcacc ccagcggtgg agctcggggc aggccgggtt gagcggctcg
      361 gcagcgaccg catctgcgtt cctgatttcg gagtccatgg ggcctgtagc gcaggcggag
      421 attgcgccgg cagctaccaa aagaatagcg tatcggatca ttggaacgac ttcatggggc
      481 cgcctgcggc ggtgcactgg cggcgcgctg ggcaaactcg gcgcgcagcg ccttgagctt
      541 ggcacgcggg tcctcgcgca cggtggctgc gtccagcgcc atttcggcga tgaagcggct
      601 gggctgggcg gccaccacct cgcggccctt cttgcgccgg cgcgtccagc tcacggccag
      661 ggtgttgcgg gcgcgggtga tgccgacgta catcagacgg cgctcttcct cgatgcgcgc
      721 ggcctgggcg gcggcgccgg cgtcctcgtc ggggcgaaac ggcagcaggc cttcgttgac
      781 gccagccagc accacgtgcg gccactccag gcccttggcg gcgtgcaggg tggacagggt
      841 gacgacgttg gggtcctgct cgcgttcgtt cagcgtggag atcagcgaga tggtctgcgc
      901 gacttcgagc aggctcctgg attcctggtg cagcgtgacg cccgcggcgt cctcgatctg
      961 gccgccgcag cgcccggcca tccagtcgca gaactccagc acgttggacc agcgcgcggc
     1021 ggcctgcggg gcgctgtcgg cgctgtcatg caggtgctgc tcgtagccga tgtccttcag
     1081 ccagtcggcc agaaaagtgc gcgccgcctc gtggccgtgg gtgtggccgg cgcggtggcg
     1141 caggtcgttc acgaggcgcc cgaactcgtg caggctggcg atggtgcgcg ccggcagcgc
     1201 ggccggcagc gagtgcgcaa acagcgcgcc aaacagcgac agatgcgcct tgccggcaaa
     1261 ttcgcccagc gcggccaggc tctggtggcc gatgccgcgc ctgggggtgg tgacggcgcg
     1321 cagaaaggcc gggtcgtcgt cctcgttgat ccacaggcgc agccaggcgc acaggtcctt
     1381 gatctcggcg cggtcgaaaa agctttgccc gcccgagacc ttgtagggga tctgcgcccg
     1441 gcgcagcgcc tgttcgaaca ccttggcctg gtggttggcg cggtacagga tggcgaagtc
     1501 gcgccagtcc ttgtgcgcgg aggtctggcg cagcgactgg atgcgcgcca cggcgcgctc
     1561 ggcctcgtgc tgctcgtggt cgcaggccag cacgcgcacc ggctcgccct cgcccagctc
     1621 ggaaaacagg cgcttttcga acagcttggg gttggggccg atcacgttgt tggcggcgcg
     1681 caggatggcg gaagtcgacc ggtagttctg ctccagcttg atgaccttga gccgcgggta
     1741 gtcctgcggc agccggcgca ggttgtccag cgtggcgccg cgccagccgt agatgctctg
     1801 gtcgtcgtcg ccgacggcgg taaagctgcc ctgcgggccg accagcagct tcatcagctc
     1861 gtactgcgtg gcgttggtgt cctggtactc gtccaccagc aaatgggcca ggcgcgcctg
     1921 ccacttgtgc gccacatgct catgagtttg tagcagacgc aggggcaggc cgatcaggtc
     1981 gtcgaagtcc acgctctggt aggcgctcag ccgctcctgg tagcgcgcca tcagcaacgc
     2041 cgtctggcgc tcgccctcgc ccctggcttg cgccagcgcc tgcgcggcgt ccaggccggc
     2101 gttcttccag ccgctgatga cccattgcca ctggcgcgcg gtggcggcgt cggtggtggc
     2161 gccgcagtcc ttgagcaggc cgagcacgtc gtcgctgtcg aggatgctga actggggctt
     2221 gaggcccagt gtggcgccgt cctcgcgcag catgcgcaca cccagcgcgt gaaaggtgca
     2281 gatcagcacc tcgcccgccg ccttgcccac caggccgcgg gcgcgctcgc gcatttcggc
     2341 ggcggccttg ttggtgaagg tgatggcggc gatgcgcgcc ggctccaccc cggtggcaat
     2401 caggcgcgcg atcttgtgcg tgatgacgcg cgtcttgccc gagccggcgc cggccagcac
     2461 caggcagggg ccgcgcaggt ggtgcacggc gtcctgctgg gcgaggttga ggcggtcgga
     2521 catgggggcg ccgatgatag ccgccgcggc ctgctacgct tgcgctccac atgcacgcgc
     2581 tggccggcat cttttccgtc accttcccct tctttgccct ggtgctgtgc ggctacctgg
     2641 cggcgcgctc gcgcctgctg ccgctggatg ccgtgccggg gctgaacatg ttcgtgctgt
     2701 acttcgcgct gccctgcatg ctgtggcgct tcggcagtgg cacgccgctg ctgcagctgt
     2761 ttcaccctct gatcgcgctg ctgtggctgg cggcggcggc gctgatcgtg gccggcaccg
     2821 cctggctggc gcgccggcgc gggggcggct ggccggatgc cgccatgggc gcgctggtgg
     2881 cggcctttcc caattccggc ttcatgggcg tgccgctgat cctggcgctg ctgggcgagg
     2941 ccgccgtcgg cccggtcatg gccacgctgc tggtggacct ggtggtcacc acgtcggtgt
     3001 gcatcggcct gtcgcaatgg ggcgcggccg acgagcacgg cgccgccaag gcggcggggc
     3061 gggccttgca gggggtgctg cgcaacccga tgccatggtc gatcctgctg ggcgcgttga
     3121 ccggtgcgct gggcctggcg ccgtgggcgc cgctggcgcg caccgtcgat ctgctggccg
     3181 acgccgcttc gccggtggcg ctgttcacca tcggtgcggt gctggcgcgc tcgcaagtcc
     3241 aggcgcagac ccgtgcccag tccggcgcgg ccgggttggg tgacgtcggg gtgatcacgc
     3301 tggtcaagct gctggtgcac ccgctgctgg tgtgggcgct gggctggctg gccgtgcgtg
     3361 ccggcgggct gacgccggcc accgtgacct cgctggtgct gacggcggcg ctgccggcgg
     3421 ccggcaacgt gtcgctgctg gccgagcgct tcggcgccga caacgggcgc gtggcgcgca
     3481 tcatcctgtg gtcgacggcg ctggcgttct tcagtttttc gggggtggtg gcgctgcgtc
     3541 cgtgagcgcc tcggcgcctt gcgccgccag cgcctgatgg agcagctggg tcagccgccg
     3601 cgcggcgcgc tcgatctgct ggcggatcac cggcacgctg gcgcgctggt agtcggcgtc
     3661 cagccgcagc ggttcggcca gtcggatgcc gcaggcgaac ccgttcagcg gctgatagac
     3721 ctgggtcagc gccacgtcgc gcgtctccag catccagccg gtcggcgcat cgagcgccag
     3781 cgcggcggtg tcggcggcgg gcgtgcgcgg ttgccagccg gccagcgcgg cgtccagcag
     3841 cgcaccgtcc cagaccgcgt gcaggctggc gtcggcgggc ggggtggcac cgaccagctc
     3901 cacccgcgcg cggccgccgt tggggttgac gcccgagtgc agcggctggt gcagatcgcc
     3961 caccaggtgc accacccatt tcagcgcttc gttgcgctgc cgcagcggct gcgtgtggtc
     4021 gcgcagcacg gcggtgtggt ggatgatcag ttgatcgacg caatgcccct gcgggcagcg
     4081 gcccaggcgc tcgccacaga ccgggttggc gcgcacgtgc cagccgcggt aggcctcggg
     4141 gtcgcggcgg gccgcttcgg cgcggatctc gtccggccac gacgccaccg ccgccagcgt
     4201 gcggcgcccc gatggccgtc cgaagcggtc caggtcctgc gccagcagcg cctgcacctg
     4261 tgcgcgggcg gcgtcgttca agccggcttc ggccagcgcg gcgatcgccg catggccgcg
     4321 cgcattccag gcgccggccg gcaggccgcc ggcgctcagc accagcgcca gccctgcgcg
     4381 gcacagccag gcccgtcgtc gcgggtgggg tggcggcgtc atcgcgggcc gtgcagtgtc
     4441 gaaccgatgg ttccggaggc ggaggtcact catagcgcca gcggatcgac gtcgatcgcc
     4501 cagcgcacga tgccgcggtg cgcgggcacg gtgcgcagct ggtgcagcag cggagggcag
     4561 gcggccagca cgcgctgcag tgcggcgcgc gaggggcttt ccagcaacat ctgggcgcgc
     4621 tcgatgtcgg ccacgcgctg caccgccagg ggtacggccg ggtaccaggt gacctgcgcc
     4681 agcgcctcgg ccagctgcgg gtcggggtgc gctggctgct gagcggcctg cgccgtggcg
     4741 gcgagaaacg cctgcgccgc cgcctgtgtg cgcgcctcgg cgcgcagcag ggccaggcgg
     4801 ctgaacggcg gcaggccggc ctgggcgcgc tcggccagct ggcgggcggc gaagccgggg
     4861 tagtcgtggg ctttcagcgc ctcgaacagg ggatgctggg ggtggtgggt ctgcacccac
     4921 atttcgctgc cggcggcgct ggcggcgtcg cggccggcgc ggccggcggc ctgcaggagc
     4981 agggcgaaca gccgctcggg ggcgcgaaag tcgctggaaa acagggccgc gtccggctgg
     5041 atggcggcca ccagggtgac gcgcctgaag tcgtgcccct tggcgatcat ctgcgtgccc
     5101 accagcacgt cgatgtcgcc ggcgtgcacc tgggccaggg cgctgtcgag ctggcccttg
     5161 ccgcgcgtgc tgtcggcgtc gatgcgggcg atgcgcgccg gcgcgccgtc ggggcggcgc
     5221 acatcggcca gcagaccggc cagctgcgct tcgagctgct cggtgccacg gcccaggctg
     5281 ccgaggtcga ggttgccgca cgcgggacag gcgcgtggca ccggttgggt gaagccgcag
     5341 tggtggcagc gcagcgagcg gtcgatccgg tggaatacgc ggtaggcgct gcaatgcggg
     5401 cactcgcttt tccagccgca gtcggcgcag tgcagcaccg gcgcccagcc gcggcggttg
     5461 agcagcagca gcgcctgctc gccacgcgcc acgcgctcgc cgatggcggc cagcagcggg
     5521 gcggcgatca gcgtgcccct gggctggcgc gacatgtcga cgcggcgcac gcgcggcagg
     5581 ccggtggccg ggtcgtcggc gggcgtcagg ccgacgcggc gcggcatggc caggcgccgc
     5641 aggcggccgg ggccttcggg tgcgggggcg ctggcgtgcc agctttccag cgacggcgtg
     5701 gccgagccca ggatcacctg cgcgccggcc aggcgcccgc gcatcagcgc caggtcgcgc
     5761 gccgagtagc gcgcgccttc ctgctgcttg tagctgggat cgtgctcttc gtccaccacg
     5821 atcagcgcca gctgcggcat cgacgccagc accgccaccc gcgtgcccag cacgatgcgg
     5881 gcgcgcccgc tgtgcgccgc cagccaggcc gccaggcgct gcggcggcgt caggccgctg
     5941 tgcagcgaca cgcaggcggc ggcgccgaag cgggcggcaa agcgcgcttc cagctgcggc
     6001 gtcaggttga tctcgggcac cagcaccagc acctgcgccg ccggctggtg cgccagcacg
     6061 cgcgcggcgg cctgcatata gacctcggtc ttgccgctgc cggtggcgcc gtgcagcaga
     6121 aacgggcccg gctcctgcgc gatgcgggcc agggcggccg cctgctccgc cgtcagcgcc
     6181 gttggcgctg ctgctgcggc ggcgccggct ggcggcgcct cgcccaggcg cctgaggcgg
     6241 cgcgccatct gggcgctgct gagcgtgcgc agctgcggcg gcagcgccgc cagcgccact
     6301 tcgcccaggg cgcgctggta gtagctggcg gtgaaggcga tcagccggcg ccagtcgtcg
     6361 gacagcggcg tcaggccctc gaacacgccg gccagcggac gcacctgggc cgcccagtcg
     6421 ggcgcggggg tggccgctgt gccccacgcc acgcccagca cctcgcgcgc acccagcggc
     6481 acgcgcacga tgctgccggg cgccagcggt gcgtcggcgc ggtaggtcag cggctcgccc
     6541 aggccgctgt gtgccggggt ggcgacggcg acggccagcc agtgcgtcat gcggtgaacc
     6601 tgatctgggt catggcttgc cgacacttgc agtttcgggc ttaagtgctt gttttgaaag
     6661 cgtctgccaa gtcatgccca gaatatgtgg ataactttgt tgagaattcc ccggcgctcg
     6721 cccgcaggcc ttgaaaatca agggtttcct tatcctgcct gccaaagcag caacacttga
     6781 agtccaatca aatcaataac ttagctgcgc ttcgcgtttt gtagcgcggg gtcgtgggcc
     6841 gggccttccg atgcgggtgc acgccggttt ttgtgcataa gtcaagcccc cgagttgctg
     6901 gatgaagtgt gaaccatgcc cgctgcgcgc ttgacacggg gcggccgcag cagtaggcgg
     6961 gtgcccgtgg ccgtggcggc gcagcggcgg tcaggccgac cgcgcccgca gcaggcgcga
     7021 atggcggtgc acctcatcga ccaggtgcgc cacatgctcg ggcggcgtgt gctggctgat
     7081 gccgtggccc aggttgaaga tgtgggtgct gcccggcacg gcggggtcgc tgtgcgggcg
     7141 gccgaagctg tccagcacgc gccgcacttc ggcgcggatc gcctccggcg gtgcgaacag
     7201 cacgttgggg tcgatgttgc cctgcagcgc cttggggtgc cgggatgtag catcgttttg
     7261 gcctccggcg cttgctgggc aagcgccagc agctataaga gttgtagcat tctgggcaat
     7321 ctgcgtgagc atgcggcgcg ccgtgcccag gttcacggtc cagtccaggc ccagcacgtc
     7381 gcagtccagt gccgccatgt cggacagcca cagtccgccg cccttggtga agacgatgcg
     7441 cggcaccacc tgaccgtcgg cgccggtgcg cttgagctgg cccagcacgc gcgcggtgta
     7501 ggccaggctg aaggtctgga acgcgccgtc ggccagcacg ccgccccagc tgtcgaacac
     7561 catcacggcc tgcgcgccgg cgtcgatctg ggcgttgagg tactgcgcca cggcgtcggc
     7621 gttgacggtc agcaggcggt gcatcaggtc gggccgggcg tacatcaggg tcttgacgtg
     7681 gcggtagtcg tcgctgccgc cgccttcgac catgtaacag gccagcgtga aggggctgcc
     7741 cgagaagccg atcagcggca cacggccatt caaggccttg cggatgctgg tgacggcgtc
     7801 gaacacgtag cgcagccggt ccatgtcggg caccgccagc cgcgccacgg cggcttcgtc
     7861 gcgcaccacc tgcgcaaact tggggccctc gccctgcgca aacgacaggc ccaggcccat
     7921 ggcgtcgggc accgtcagga tgtcggaaaa caggatggcg gcgtccagcg ggtagcgctc
     7981 cagcggctgc agcgtgacct cggtcgcgta ttgcacgttg gtggccagcc ccatgaagct
     8041 gccggccctg gcgcgcgtgg cgcagtattc gggcaggtag cgcccggcct ggcgcatcag
     8101 ccagaccggg gtgtgctcgg tggcctggcc cagacaggcg cgcaggaagg tatcgttgtg
     8161 caaaggggca aagctggtca tgcccggatt gtctcagcct tgcacgtggc acccccgcac
     8221 aatgtcacgc cagcgcgggc ggcggccgcg ccgggccgtc cgccaggccc aaaacccctt
     8281 cacaatacgg gttaaccctt ggctttgacc caaggcgcgg gacagcacca tggaccagca
     8341 ttacctcacc cccctgttcg atccgcgttc gatcgccctc ttcaccggcc cgcaggacgc
     8401 gcccgagcag cagacgccgc aggcgcgcgc gctgctggcg catctgcagg cgcaggcctt
     8461 caagggccag gtggtgccgc tcagcgtcca caccaccggc accctggccg acctggcgca
     8521 ggcgcgcgcc gacctggcca tcatcgccct gccggacgaa gaaatcggcc ccgcgctgga
     8581 gctggccggt cgcatcaagg tgaaggcggc gctggtgctg tcccatggcg tggacgcccg
     8641 gcgcgcgcgc gaactgcagc ggctggcgcg ccgccacggg gtctggttgc tggggcccaa
     8701 cagccagggc tttcagcgcc ccggtctgca gctcaacgcc agcgtggcgg gcgcgctggc
     8761 ggcgcctggg caactggcgc tggtgtcgca gtcgggcgcg ctgacggcgt cgatcctgga
     8821 ctgggcccgc cagagtcggg tcggtttttc caccgtggtg gcgctgggcc cgcacaccgg
     8881 cgtgacgctg gccgacacgc tggactttct ggccgccgac cgccacacca gcggcatcgt
     8941 ggtctacatg gaaggcatcg ccagcgcgcg ccgcttcacc agcgcgctgc gtgcggccgc
     9001 caacgccaag ccggtggtgg tgctcaagtc cgggcggcag gcggccggca acgtggcggc
     9061 gcagacgcat tcgggcgcca tcgtcggcag cgacgacgtg ttcgacgccg tgctgcggcg
     9121 cgccggcgcg gtgcgcgtgc gctccttcgt cgagctgttc tcggccgcca agtgcctggc
     9181 ctcgcgctac cgcccggtcg ggcggcgcct ggccatcatc agcaacggcg gcggccccgg
     9241 cgtgctggca gccgactggg tgagcgagct ggggctgacg ctgggcgtgc tgtcgcccga
     9301 ggcggcgcgc gagctggcgc cgcggttgcc ggcgcaggct tcgctgagcg acctgatcga
     9361 cctgtcggag ggcgccacgg ccgagcattt ccgcgccgcg ctggaggtgg cggccaagga
     9421 ccgcgccgtg gacggcgtgc tggtgctgta ttcacccaag ttcggcggcg acgccgacgc
     9481 cgtggcgcag gagctggtgc aggcccgccc gcgcctggcc aagccgctgg tggcctgctg
     9541 gatgggcgac gccagcgtgc aaacggcgcg ccacgccctg gccgaggcgc agatccccag
     9601 ctttcgcacc cccgaggcgg cggtgggcgc cttcggcaac atcgccagct tctatcagaa
     9661 ccagcagatg ctgcagcaga cgccgccgcc gctcaccggc ctggcggcgc ccgacgtgga
     9721 aggcgcgcgc ctgctgatcg agagcgtgct gaccgagcgg cgcagcgtcc tgaccgagat
     9781 ggaatccaag gcgctgctgt cggccttcca cattccggtc acgcagacgc tgctggcgcg
     9841 cagcgccaac gaggccatga tgatcgccac ccagatcggc tttccggtgg cgctgaagat
     9901 cgactcgccc gacatcagcc acaagagcga cgtcggcggc gtggcgctga acgtgatgca
     9961 cgccaccgcc gtgcgcgaca tctggcagga catgatggcg gcggtgcgcg aacgccagcc
    10021 cgacgcccgc atcaacggca tcacggtgca ggccatggcg cgcaagccgc gcgggcgcga
    10081 gctgtacatc ggcgtggtga ccgacgagcc gttcggcccg gtgatcgcct tcggcgccgg
    10141 cggcaccctg atcgagctgt accgcgacca tgccatggag ctgccgccgc tcaaccagtt
    10201 cctggcgcgg cgactgatgg agcgctcgcg cgtgcacgaa accctgggtc cctggcgccg
    10261 tgcgccggcg gtggacatgg cggcgctgga gcaggtgctg ctgcgcgtgt ccgaaatggt
    10321 gtgcgagctg ccgcagttgc gcgagatgga catcaacccc atcatcgtcg acgaacacgg
    10381 cgtgctggcg gtggacgcgc gcatcgtcat cggtccggcg gcgcaggccg caccggggcg
    10441 cgcggggcgc tacggtcatc tgtcgatcct gccgtacccg gcgcgcttcg agcagtggtg
    10501 gccgctgcgc ggcggtggcg aatacaaggt gcgaccgatc caccccgacg acgcgcagcc
    10561 gctgcaggac atgatgaagc gcctgacgcc cgagagccgc tacttccgct tcgtctccaa
    10621 catggccgag ctgccgccgc acatgctgtc gcgcttcacg ctgatcgact acgaccgcga
    10681 gatggcgctg tgcgccgtgg tggaagagga ggtgccgacc gaggacggcg gcaagaccac
    10741 gcgcgagcgc atcatcggcg tgtcgcgcta catcaccaac cccgaccaga ccagctgcga
    10801 gttctcgctg gtggtggacg acggctacgc cggccagggc ctgggcacgc ggctgatgct
    10861 cagcatcatg gacgcggcgc gcgaaaaggg cctgcaggaa atcatcggcc tggtgctgac
    10921 gcacaacaac ggcatgctca agctgatgcg cagcctgggc ttcgaggtca aggcctaccc
    10981 ggaagacccg gacttccggc tggtcacgca cccgctctga agcggcccgg gccccgcatg
    11041 ccgccgtgcg tcccggtcgt tcgtcattgc ctgcgcgccg gttcaggtca gcggtccagc
    11101 agatcgcgcg ccacgatctc tttcatgatc tccgaggtgc cggcgtagat gcgctcgatg
    11161 cgcgcatcgg cgtaggcgcg tgcgatcggg tactcggcca tgtagccgta gccgccgaac
    11221 agctgcaggc attcgtcggt gacgcggccc agcagctcgc tgtgccacag cttgccggcg
    11281 gcggcgccga cggcatccag cccgccggcc aggtgctgcg cgatcagctg gtcgcagaag
    11341 gcgcggccgg cggcgatgtc ggcggccagc tgggccagct tgaaacgggt gttctgaaag
    11401 tcggccaggc gctggcggaa cgcccggcgc tcctgcacgt aggccacggt ccagtccagc
    11461 accgcctcgg cgcgcgcctg gcactgcacg ctgatcagca ggcgctcctg cgccagcttc
    11521 tgcatcaggt acttgaagcc ctggccctcg tcgcccagca ggtggctggc gggcacgcgc
    11581 acctggtcga agaacagctc ggccgtgtcc tgcgcgtgct ggccgatctt gcgcaggttg
    11641 cgcccgcgcg tgaagcccgg catgtcgcgc tcgaccacca gcagcgacac gccatgcgcg
    11701 ccctgcgcgg ggtcggtctt gcaggccacc accaccaggt cggcgttgtg gccgttggtg
    11761 atgaaggtct tctggcccgt cagcacatag tgttcgccct cgcgccgtgc ctgggtgcgg
    11821 atggccgcca cgtcgctgcc ggtgccgggt tcggtcatgg cgatggcggc gatcttgtcg
    11881 ccgcgcacca tggccggcag cagcgcctgt tgcagctcgg gcctgccatg cgcggccagg
    11941 tacggcgcca cgatgatgga atgcagcgga aagcccggcc cggtggcgcc gatgcgcccc
    12001 agctcctcgc tgacgatgaa gtcgaacagc aggtcggcgc ccggcccacc cagcgcctcc
    12061 ggcagccagc agcacaacag gccgtggtcg ccggcctggc gccagacgtc gcgcggcacg
    12121 ccgtgggctt cttcccaggc ggcgtgctgc ggtgcgatct cgcgctcgca gaagcggcgc
    12181 acctgctcgc ggaaggcgtg gtggtcgggg ctgaacaggg ggcggggggc gggtttcatg
    12241 ggctgcactg taacccgact gtcgggccac ccggggcctg tcggaaaata tcagaatgat
    12301 agctggcggc gcttattcga cgggcgctgg agccctgttg gattcagcat cgccgtgcag
    12361 gcgccagccc tgcggcgtgt ggccggtcca gtggcggaag gagcgcgcaa agctcttgtc
    12421 gctggcaaag ccggccgcac gcgccacgcg ggcgatcggc cagtcggtgc gcagcagcag
    12481 cgcgcaggcc tgggcgcgcc gtgcttcgtc cttgagctgc tgcacgctgg tgccttcggc
    12541 gcgcaactgg cggtgcaggc tgcgcgccga cagcgccagc cgcgccgcca gcgtctcggc
    12601 ggtatgcgcc gcctgcggct gctgcagcag ggcgcgcacg cgccgcgaca gcaggcggtc
    12661 gcgccggtag ggccagacca ggatcggcag cgcgcggcgc agcatctggt ccagcgcggt
    12721 gccgtcgcgg cgcagcggca actgcaggta gtgggcgtca aagcgcagcc cggcgcaggt
    12781 ggcgccgaac cgcaccgggc cgggaaacag acgcgcgtac aactcggcat gtgccggcgc
    12841 ggcgaacgga aactgcgcgc cctgcaggac gatctgcgaa tccaccagcc agcaggccag
    12901 cccgtgcagg ttgcgcagca gcgacagcag ggcgaactcg cgcagcgcgc ccagcgcgcg
    12961 ccgctccacg atctgcacct gggcctgcgc gccgctgcgc tccagcgtca gcgccacgtc
    13021 gtcggtcagc aggccatggt ggcggcacca gcgcgccagc gccacgccca gcgtgggcgc
    13081 gctgatgctg gcgcgcgcca gcatgccgta gctgccccag ggcaggcggc ggctgaacca
    13141 gccgagcgcc tcgtcgtcca gctcctgcat ggcggcggcg cacaggcgct cgaactgcag
    13201 ggcggtgacc tgggtgcctg gccgtgccag atcggatggc gcgatttgtg ccttcagcag
    13261 agccgcggcg ggatccagtc ggcgctgcgt gtaggcctgc accaccgcct gcaccagaga
    13321 gatcggggtc tgcccggcag ggcgcggcgg gcggtcgggc gtggcagcgg gcggcattcc
    13381 ccaagtgtcg gcatggctgg cacattttgc aacccgtctg gcaacacccg gcccctggcc
    13441 ggggctaagc tggcgcctgt acgggcggcc cgcggctgca ctaccatggc ggccagcctg
    13501 gcccggatcc gcaggagacg tgaaatgagc acggcactga ccgaaagctg cgcgcgcggc
    13561 gcgcagacgc cgccgctgat cgagcaaacc attggcgcct ttttcgacgc catggtggcg
    13621 cgctttcccg agcgcgaggc gctggtggtg gcgcaccagg ggcggcgata cagctaccgc
    13681 cagttgcagc aggacgccca ccggctggcc agcgcgctgc tgcggctggg cctgaagccg
    13741 ggcgagcgca tcggcatctg gtcgcacaac aacgccgaat gggtgctgat gcagctggcc
    13801 acggccaagg ccggcctggt gctggtcaac atcaacccgg cctaccgcac ggccgaggtg
    13861 gaatacgccc tcaacaaggt gggctgccgg gcgctggtga ccatgccgcg cttcaagacc
    13921 agcgactatc tgggcatgct gcgcgagctg gcgcccgagt gggcgcacgc caggccgggc
    13981 gagccgctgc agtcgcagac gctgccgcag ctgcagcacg cgatctggat cgacgttgcc
    14041 ggcgaaggcg ccgaggagcc gggcttcatg cgtttttccg agctgctggc gagtggcgat
    14101 ccgcaggacc cgcgcctggc ccaggtggcg cgcctgctgc atccgcagga cgccatcaac
    14161 atccagttca ccagcggcac caccggcttt cccaagggcg ccacgctgac gcaccgcaac
    14221 atcctgaaca acggcttctt catcggcgaa gcgatgaagc tgaccgagca agaccgcctg
    14281 tgcattcccg taccgctgta ccactgcttc ggcatggtgc tgggcaacct ggcggtgctg
    14341 acgcatggcg gctgcatcgt ctatcccaac gacgccttcg acccgctcac cgtgctgcag
    14401 accgtgcagg acgagaagtg caccggcctg cacggcgtgc ccaccatgtt catcgccgag
    14461 ctggaccacc cgcgcttcaa ggaatttgat ctgtccagcc tgcgtaccgg catcatggcc
    14521 ggcagcccct gcccgatcga ggtgatgaag cgcgtgcaga gcgaaatgaa catgggcgag
    14581 gtgaccatcg cctacggcat gaccgagacc tcgcccgtca gctgccagag ttccaccgac
    14641 acgccgctgg acaagcgcgt gtccaccgtg ggcctggtgc agccgcacct ggagatcaag
    14701 atcatcgacc ccgagaccgg ccgcaccgtg ccggtgggcg agcgcggcga gttctgcacc
    14761 cgcggctatt cggtgatgca gggctactgg gaagacccgc agcgcaccgc cgaggcgatc
    14821 gacgccgaag gctggatgca caccggcgat ctggcgacca tggacggcga gggctacgtc
    14881 aacatcgtcg gccgcatcaa ggacatggtg atccgcggcg gcgaaaacgt ctatccgcgc
    14941 gagatcgaag aattcttgta ccgccacccc aaggtgcagg acgtgcaggt ggtgggcgtg
    15001 ccggaccaga agtttggcga ggagctgtgc gcctggatca tcgtcaagcc cggccagcag
    15061 ctcaccgacg ccgaggtgcg cgagttctgc aagggcaaga tcgcgcacta caaggtgccg
    15121 cgctacctga agttcgtgcc cgagttcccc atgaccgtga ccggcaagat ccagaaattc
    15181 aagatccgcg aggccatggt ggccgaactg ggtctggtgc aggaccagac ggcctgaaat
    15241 ccaaaactgg accgaacgca gagaacgcaa aattcacgca gaggacgcaa aagagcagcc
    15301 catttgattc agcatttttt ctgcgccctc tgcgaaacct ttgcgccctt tgcgttcggt
    15361 attcaatttt catttgagcc ccgcccatgc ccgccatcga caccaaactg aacccacgct
    15421 cggccgactt ccaggccaac gcccaggcca tgcgcgcgct ggtggacgat ctgcgcgccc
    15481 aggtgcgcaa ggtcagcgaa ggcggcggcg aggcggcgcg caagaagcac atggaccgcg
    15541 gcaagctgct gccgcgcgtg cgcgtgcaga tgctgctcga ccccggcaca ccctttctgg
    15601 aaatcgcccc gctggcggcg ctgcacatgt accaggaccg caacggccag gacgaggcgc
    15661 ccgccgccgg catggtggcg ggcatcggtc gcgtttcggg cgtggactgc atgatcgtct
    15721 gcaacgacgc cacggtgaag ggcggcacct actaccccat gacggtgaaa aagcacctgc
    15781 gcgcgcagga gatcgcgcag caaaaccgcc tgccctgcat ctacctggtc gacagcggcg
    15841 gcgccaacct gcccaaccag gacgaggtgt ttcccgaccg cgaccacttc ggccgcatct
    15901 tctacaacca ggcgcagatg agcgcccagg gcattgcgca gatcgccgtc gtcatgggtt
    15961 cctgcactgc cggcggcgcc tatgtgccgg cgatgagcga cgagtccatc atcgtcagga
    16021 accagggcac catctttctg ggcggcccgc cgctggtcaa ggccgccacc ggcgaggagg
    16081 tgagcgccga agacctgggc ggcggcgacg tgcacacccg cctgtcgggc gtggccgacc
    16141 acctggcgca gaacgacatg catgcgctgg cgctggcgcg gcaggtggtg gcgcacctga
    16201 accgccacaa ggcgccggcg ggcgatctgc gcacaccctg tccgccccgg tacgccgccg
    16261 aggagctgta cggcgtgatc cccaccgaca cccgcaagcc gttcgacgtg cgcgaaatca
    16321 tcgcccgcat cgtcgatgac agcgcgttcc aggagttcaa ggcgcgcttt ggcaccaccc
    16381 tggtgtgcgg ctttgcccac atcgagggca tgccggtggg catcgtggcc aacaacggca
    16441 tcctgttttc cgagtcggcg caaaagggcg cgcacttcat cgagctgtgc tgccagcgca
    16501 agattccgct ggtgttcctg cagaacatca ccggcttcat ggtcgggcgc aagtacgaga
    16561 acgaaggcat cgcccgccac ggcgccaaga tggtgacggc cgtggccacc gccaacgtgc
    16621 ccaagttcac cgtcatcatc ggtggcagct ttggtgccgg caactacggc atgtgtggcc
    16681 gcgcctacag cccgcgcttc ttgtggatgt ggcccaacgc gcgcatcagc gtgatgggcg
    16741 gcgagcaggc cgccagcgtg ctggccaccg tcaagcgcga cggcatcgag gccaagggcg
    16801 gcagctggag ccaggacgag gaggaggcct tcaaggcccc gatccgcgcg cagtacgagc
    16861 agcagggcca cccctactac gccaccgccc gcctgtggga cgacggcgtg atcgacccgg
    16921 ccgacacccg ccgcgtgctg gcgctgggcc tgtcggcggc gctgaacgcg cctgtggaag
    16981 acgtgaagtt tggcgtattc cgcatgtaag gtgtacgatg tgtctcaagt ggtgaatgta
    17041 tacacattat gcgcaccaac atcgacatcg acgacgaatt gctggccacg gccatgcgtg
    17101 ccggccccta caccaccaag aaggaaaccg tggaggctgg cttgcgcctg ctggcgcgac
    17161 aggcggccta ccgcgagatc ctcaagtgga agggcaagct gaaatgggaa ggcgacgagt
    17221 ccatcgattg gacgcggccg gccgcggacg agcccgagga agcactggag tacagcgtgc
    17281 agcagccgcg cgcccgccac accaagacga aagccgcggc gtccagcggc aggaaggcgg
    17341 cgcgcggtgc tggtcgttga ctccagcgtc tggatcgact tcttcgtcga tcggcccacg
    17401 gcggcgtgcg acgagcttga tcgcttgctg gccgatggcc acacccgcct ggtggtgccc
    17461 gacctggtgc tctacgaggt gctgcgcggc tttcgcgacg agcgagcgct gcgtcaggcg
    17521 cgtctgttgc tgcaaacctt gagcgtcgag tcctgcggcg gcgccgacat cgctctggcc
    17581 gccgccgagc actatcgcct gctgcgcgcg cgcggcatca cggtgcgcag cagcgtcgac
    17641 gtgatgatcg ccgccttctg catcgagcag gactatctgc tgctgcacag cgaccgcgac
    17701 tttgatgcca tcgccgcgca ccgcggcctg cgcgtctggg tgcattgacg atgccggcgt
    17761 ggcacagcca tttcagcacc ctggcgcgct acaacgcctg ggccaccggt cgcctgctgg
    17821 ccggcgtggc ggcgctgcct gaagacgact accggcgcga cgtcggcctg tttttcaaga
    17881 gcatccacgg cacgctcaat cacctgctgg tgggcgagca cctgctgtgg tttcgccgct
    17941 ttgccgaagg cgtctcgccg cggctggcgc tggatgacga agccgagacc gaccgcaccc
    18001 gactggccga gcgcctgcgg caaggtgcgg cgcgctggca gccgctgatc gcgtcgtggc
    18061 cggccgaacg cttcgacggc gaactgcact acaccaccat gcgcggccag gccgtggcgc
    18121 tgccctttgc cgccacgctg gcgcatgtgt tcaaccacgg cacgcatcac cgcgggcaga
    18181 tcacggcggc gctgacggcg ctgggccagc catgccctga acttgatctg gtttatctgc
    18241 tgcaacagga gtcgcgccaa ccatgaccgc cgcccttcgc atcgacgtgc aagaccacgt
    18301 cgccaccgtc acgctggcgc gccccgagct gcgcaacgcc ttcaacgacg ccgtcatcgc
    18361 cgagctgtcc gaggccttca ccaccctggg cggcgacgcg cgggtgcgcg ccatcgtgct
    18421 ggcggccgaa ggcccggcct tctgcgccgg cgccgacctg aactggatgc gccgcatggc
    18481 cgactacacg cgtgaggaaa acctgcgcga cgcggggcaa ctggcgttca tgctgcgcac
    18541 cctctacgaa tgtcccaagc ccaccattgc ccgcgtgcag ggcgacgtgt acgccggcgg
    18601 cctggggctg gtggcggcct gcgacatggc ggtcagcgtg gacagcgcgc acttctgcct
    18661 gagcgaggtc aagctgggcc tgatcccggc caccatcagt ccctacgtca tccgcgccat
    18721 ggggccgcgc gcggcgcacc gctggttcct gaccgccgaa cgcttcgacg ccgccgaggc
    18781 gcggcgcctg ggcctggtgc acgaggtggt gggtgccgag cggctcgacg ccacggtggc
    18841 cgggctgctg caggcgctca ccagcgccag cccgaacgcg gtcagggcct gcaagcagct
    18901 ggtgcaggac gtggccgagc gcgacatcac gcgcctgctg atcgagcgca cggtggaggg
    18961 catcgccgac atccgcgcca gcgacgaggg caaggaaggc gtgcagtcct ttctgcaaaa
    19021 gcgcaagccg gcgtggctgg catgacccat tcgaacccat gagttttcaa atgatttcgt
    19081 cctctggcgc ttgtccatca agcgcaagca gctattgaat tgatagtcct cgatttctcg
    19141 atcctcatca gaattcatgc ccgattggct tgcttcattc atccagtggc tgcacggcat
    19201 gggcctgcac ctggggccgg attcggccgc ggcgctgggc caggcggccg aatcggccag
    19261 ccaggcggcg gcgcgcatgg acatgccgca gctgctggcg ctggcggccg cgctgggctg
    19321 ggccagtggc tttcgcctgt acgcggtgat gtttctcacc ggcgccatgg gtgcggccgg
    19381 ctggatcgcg ctgccggcgg gcctgcagct gctgcagcac ccggcggtgc tggcggccag
    19441 cggtttcatg atgctggtcg agttcttcgc cgacaaggtc ccctggctcg acagcctgtg
    19501 ggacgcggtg cacagcgtga tccgcatccc ggccggcgcg ctgctggccg gcagcgtgtt
    19561 cggcgccgat tcggccacca tggccgtggt ggccgggctg ctgggcggct cgctggccac
    19621 caccgcgttc gccaccaagg ccaccacgcg cgccgccctc aacgcttcgc cggagccgtt
    19681 gtccaacggg ctggcctcac tgttcgagga cggtctggtg gtggccgtgg tgtggctggc
    19741 cacgcagtac ccgctggcct ttggcatcgc gctggcggtc atgctgctgc tgtcggcgct
    19801 gctgctggtg tggctggcgc gctttctgaa gttggtcgtg cgcaagttgc gcggcttttt
    19861 tggtcatcaa gtaccgggtc cgaccgacgc acgggtctga tccggttggc aaggagaaaa
    19921 gcatgttcac caagattctg atcgccaacc gcggtgagat cgcctgccgc gtggccgcca
    19981 ccgcacgccg cctgggcgtg cgcacggtgg cggtgtattc cgacgccgac gcccatgcca
    20041 agcatgtgct ggcctgcgac gaggcggtgc acgtgggcgg cagcgcgccg cgcgacagct
    20101 acctgcagtg gcagcgcatc atcgacgccg cccgcgccac cggtgcgcag gcgatccacc
    20161 ccggctacgg ctttctgagc gagaacgaag acttcgccca ggcctgccag gacgccggcc
    20221 tggtgttcat cggcccgccg gccagcgcca tcaacgccat gggcctgaag gccgagtcca
    20281 agcgcctgat ggaaggcgcg ggcgtgccgc tggtgccggg ctaccacggc gccgatcagg
    20341 ccccggccct gctgcgccgc gaagccgacc gcatcggcta cccggtgctc atcaaggcca
    20401 gcgccggcgg cggcggcaag ggcatgcgcg cggtcgagcg cgcggaggac ttcgacgccg
    20461 ccctggcgtc ctgccagcgc gaggcccgca acagctttgg cgacgacgcc gtgctggtcg
    20521 agaaatacgt gcagcgcccg cgccacatcg agatccaggt gttcgccgac acgcacggcc
    20581 actgcgtgta cctgttcgag cgcgactgct cggtgcagcg gcgccaccag aaggtgctgg
    20641 aagaagcccc cgcccccggc atgacgcccg agctgcgccg tcgcatgggc gaagccgcgg
    20701 tggcggcggc gcgcgcggtg ggttacgtgg gcgccggcac ggtcgagttc atcgtcgagc
    20761 agcctggcgg ctacgagcag cccgagcaga tgaagttcta tttcatggag atgaacaccc
    20821 gcctgcaggt cgagcacccg gtgaccgagg ccatcaccgg gcaggacctg gtcgagtggc
    20881 agctgcgcgt ggcggccggc gagccgctgc cgctggcgca ggacgaactg ctgatcgacg
    20941 gccacgccat cgaggcgcgc atctgcgccg agaaccccga caaccacttc ctgcccgcca
    21001 ccggcaccct gcacgtgtac cgcaagccgc cgcacgcggc cttcgagttc ggtgcggtgc
    21061 gcttcgacga cggcgtgcgc gagggcgatg ccatcagccc cttctacgac agcatgatcg
    21121 ccaagctgat cgtctggggc gaaacgcgcc agcaggcgct ggccaagctg gacgccgccc
    21181 tggccgccac ccacatcgcc ggtctgcaga ccaacgtgca gtttctgcgc cacgtgctgg
    21241 ccagcgactc gttcgcccgg gcccgactgg acaccgccct gatcgagcgc gagcgccccc
    21301 ggctgttcca gcaggaccgg ctgggtcgcg atctggccgt ggccgccgcc gtcgcccaga
    21361 ccgtgctggc cgagcgcgca gccgaaaccc acgacccctt cagccgcacc gacggctttc
    21421 gctcgcacgg cgtggccacg cgccgtgtcg atctggaata ccggggcgag gcggtggtgg
    21481 ccaagctgcg ctaccttcag gacggcctga tgctgcagat cggcgacgcc gaagcgcagc
    21541 cgctgtcgct taccgcgctg ccggacggcc gcatcgacct gcgctacggc accgcacgcg
    21601 agctggtgca ggtgtacccg ctgggcgagg tcgagcatgt gttcggcagc cgtggcgcgg
    21661 tgcagatcac cgagctggac ctgctctcgc gcgccgaaga aggtgccggc gaaggcggcc
    21721 gcctgaccgc gcccatgccg ggcaaggtgg tgtcgatcgc cgtccgggcc ggcgacaagg
    21781 tcagcaaggg ccaggcgctg gcggtgatgg aggccatgaa gatggagcac accatcgccg
    21841 cgccgcagga cggcgtggtg gacgaggtgc tgtacgcacc cggtgatcag gtcgccgaag
    21901 gggccgagct gctgcgcctg agccaccccg gctgagcctc ggtcaggtct tggattgctc
    21961 tatgtgtgat agctctctgc gcttgccgga tgcgccccgg aggtcgatca ggcactcagc
    22021 gatgcgggca cggcgccgag ctgctcgaac ggcatgctct gcttgaccag gatcaccgtg
    22081 cagggcgcgt ccatggcgac cttgatgggc accgtggcga cgaagcgctg cagctgcagc
    22141 ccgtgcgtgg ccgcgcccat gacgatcatg cccacgtggt tggcgcgggc gaagcgcagg
    22201 atcgcatcgg ccgggtcgcc gtcttccagc acgtggtagg agatctggtg ctgctcctgc
    22261 ggcaggccct gcgcccaggt cttgagcatg gtcagcatct ggcggtgctg ggtggtttcg
    22321 ctgcggctgg cgtcggtgct gctggcggcg cccggcgcca ccacggtcac caccgccagc
    22381 cgcgcgccgg ggcggatgcc cagcgagcgg cgcaccgcct cgcgcagcga atacagcgtg
    22441 gcgtcggtga cgtcgtggtg tggcaccgcc accatcagga tcggcacctc gtcgatcacg
    22501 cgcgtgggca gcgggctggg ctggtactgc gtgccggcgg cgcgcagcca gcgcttgaag
    22561 tgggtgcgaa agccggtgcc cttcaggcgc cggccgcgtt cggtcacctg cacctgctcg
    22621 ggctgggcga ggtcgaacgc caggtgcgcg gccgaggggt agcgctcggc cgccaccggc
    22681 tccaggcagc gcaggatcac ttcctgcagc cactccggca ggtcgggccg ccgcgcgcgc
    22741 ggcggcttgg ggtccatcca caggcgctgg cgcaggccgc ccttgctgct tggcgcgcca
    22801 aacggcagct cgccggtggc cagctcgtac agcatcacgc cgatggcgaa cacgtcgctg
    22861 cgcggatcgc cgcgcacacc caccacctgt tcgggcgaca tgtaggcggg cgagcccacg
    22921 gcttcgcgca gctcttcggc cagcaggtcg gggtagtggg cgtggaacga caggccgaag
    22981 tccagcagca ccgcgtgcag tggggcgtcg gcgggcgcgc cctcggcggc gtcgcggatg
    23041 atgacgttgg ccggtttcag gtccaggtgc acggcgttct gctggtgcat gtcgtgcgcc
    23101 gcgcgcgcca cggcggcgcc caggcgggcc agctcgtgcg gcggcagtgg ctgtccctgc
    23161 agcacctggt ccagccagtg gtccagcgtg cggccgggca cgtactccat caccaggtag
    23221 ggcagcaccg acaggtcgcc cgcggccacc aggtagggca cgtgcgggcc atccagcgcc
    23281 tgcatcatct ggtgctcgac ctcgaagctg acgatggtct cggcgccgtc gccggccgtc
    23341 atgcgcggca ccttcatcac catcgggaac ggcgccgcgc ggccgtcggc atagcgggtc
    23401 tcgtagatca gggccatgcc gcccttgtgg atcatctcgc cgataagaaa gccatcgatc
    23461 tcggcgcccg gctccagcgc gctcatcggc cctgctccag ccggtcggca aagaagggtg
    23521 gcagcggcgt ggcgcggatc gcccccgcgg cggacggcac gtcgtagggc acgcgatgga
    23581 aggtgagctg ctgcgccacg tcgtcgaaca gcgcgtacat ggcgtcggtg cggccgtcgc
    23641 gcggctggcc gaccgagccc accgtggcca gccagcgccg gtggcgcggc accggcaccg
    23701 gcacgcctgc ggtcggggtg aacggcatca gcttgccggt gccgccgcgg tagtacagcg
    23761 tctgccggtg cacatggccg ccgaacacgt agtgcacgtc gggcccgccc accgggggtt
    23821 cgcaggcggc gtccaggctg agcgccgcca gcagcggcgt ctcgacgtag tgccagcgct
    23881 ccggcgcggc ggcgctggcg tgcaccagca gcagcggcgg ctggtgcagg gtgagcggca
    23941 gctcggccag aaagcgccgc tgcgccgcac tgagctgctc ctgcgtccac agcgcccccg
    24001 cctggcccag cgtcttgccg tcgcccgctg cgtcggcggc gtacccgtcg tggttgccct
    24061 tgaccaccag cgcgccttcg gcggccaggt ccatcacccg ctgcaccacg taggccgggt
    24121 cgccgccata gccgaccaga tcgcccagaa aggccaggcg ctcggcgccg gcggcctgcg
    24181 catgggccag gcaggcgtcc agcgcctggc ggttggcatg gaggtcggaa agcagggcaa
    24241 gccgcatgag aatcgatccg gtggtcgggc ggtgggctgg ggaatttcac atcatacggg
    24301 gcggcaggcg cccgcccgcc gccgctaaac tgaccaccga catgcctgcc accgccggag
    24361 aatccccatg agcctgccca cccaagtccg cctgatcgac gttggcccgc gcgacggcct
    24421 gcagaacgaa aagcagccgg tgccggcggc ggtcaagatc gaactggtgc agcgcctgca
    24481 ggccgccggt ctgcgcgaga tcgaggtcac cagctacgtc agccccaagt gggtgccgca
    24541 gatggccgac aaccaccagg tgatgggcgg catcgcgcgc cagcccggcg tgcgctacag
    24601 cgtgctgacc cccaacctga agggcttcga ggcggcgctg gccgaccggc cgcacgagat
    24661 cgtggtgttc ggtgccgcca gcgaggcctt cagccagaag aacatcaact gctcggtggc
    24721 cgagagcatc gagcgctttg ccccggtggt ggcggccgcc cgggccgccg gcatcgccgt
    24781 gcgcggcgcc atgagctgca tcgtcggctg cccctacgaa ggcgacatcg cgcccgagaa
    24841 ggtgcgccag ctggccgggt tgatgaaggg catcggcgtc gaacgcgtcg acgtggccga
    24901 caccatcggc gtcggcacgc cgcgcaaggt gcagcgcgcc atcgaggcca cgctggcgca
    24961 ttacgacatc gaccacgtca gcggccattt ccatgacacc tacgggcagg cgctggccaa
    25021 caccctggcg gcgctggagc tgggggtgtg gaacttccag tcctcggtgg ccggcctggg
    25081 cggctgcccc tacgccaggg gcgccaccgg caacgtcgcc agcgaggacc tggtgttcat
    25141 gctgcacggc atggacatcg ccaccggcat cgacctcgac cgcctgatcg acgccggcca
    25201 gttcatcagc cagcacctgg ggcgcccgcc caactcgcgc gtggccacgg cggtgctgaa
    25261 caagcgcgcc ggctgacggg cgcccgggcg ccgcggctac catcgacctc atgaaccacc
    25321 ccacctctcc cgccctgccc gacgccatgc ggcgcgtggc cgccgtgctg gcggcgcacg
    25381 gccaccccca cgccccgatc ctgctcgacg acgccgcgcg caccgcgcag caggcggccg
    25441 atgcgctggg ggtggtggtg gggcagatcg ccaagagcat catctttcgc cgccgcagcg
    25501 acgacgcggc ggtgctggtg atcgccgccg gcgacaggcg ggtcgacgag aaaaaggtgg
    25561 cggcgctggt cggcccggtg ggccgcgccg acgccgactt cgtcaaggcg cgcaccgggt
    25621 tcaccatcgg cggcgtgtcg ccggtggccc acgccagccc gccggtggtg ctgatcgacc
    25681 gcgagctgta ccgcttcgac gtcatctggg ccgccgccgg gcacccgcac ggcgtgttcc
    25741 gcctggcgcc ggccgatctg gagcggctca ccggcgcacc ggtggccgat gtggcccagg
    25801 gctgacaggc cgtggcgccg gtgccgcgcg acttcgacac cctggcgcga caggcgcagc
    25861 aggccctgcg cgacggtacg ccgctgccgt ccccctgcat cggcgtgtgc cgcatcggcg
    25921 atgccggtgt gtgcgtcggc tgcgcccggc ggctggacga gatcggcggc tggctgcacg
    25981 ccgacgaggc cgggcgcggg gccatctggc ggcgcgtgct gcagcgccgt ggcctgcggg
    26041 ccggcgccgg cggtgcggcg cccgaccctg caaggctggc gcaaggcgag cagcgctgaa
    26101 taggctctgc ggcttcccgc acgctatgaa taacgcagct gctcgcgctg agtgcgcggg
    26161 cgctgtaagc ccatttgatt cagcattcga ggggccgacc cggcccgcgc catgcccaac
    26221 gccttcaact tcaacgcctc gccgttcgac agcctgacgc cgcaggagca gcgcctggtg
    26281 cgcgaccatg tggacgtggt ctatttccgg cccgacgagg tgatcctcga cgccggcatc
    26341 gcccccatgc acctgttcgt catcatcaag ggcgtggtgc agcagttcga cggcgaggag
    26401 ctggtggcca cctacgggcc ggacgacagc ttcgacggtc gcgcgctggt ggcgggccgc
    26461 gccagcagcc gcttcgtggc ggtcgaggag gtggtggcct atgcgctggc acgccagacc
    26521 gtcaacgacc tgatcgccgc caacgccacc ttcggcgcac tggtgttttc cgatctgggg
    26581 gccaagctca gcgccgccag ccagcgccac agcggcaacg agctgcaggc gctcaacatg
    26641 gcgcgcgtgg acgaggcctt cgtgcgcccg gcgcacctgg tggaggccga caccgacgtg
    26701 gtgtccgtgg tgcgcctgtt ccagaaggag cgcaccacca acgtgctggt gcgcgacgcc
    26761 gccagccagc cgccgcgcct gggcatgttc accaccaatg cgctgcagcg cgccatcctg
    26821 tcgggccggc cgctggagca gatcgcggtc ggcgagctga gcaactacgc gctcatcagc
    26881 gtgcgcccgt ccgacctggt gggagacgcg ctggccacca tggtgcgcca ccacatccac
    26941 cgcgtggtgg tgcgcgaggg cgagcaggtg ctgggcgtgc tggaggcgct ggatgtgttc
    27001 agcttcctgt ccaaccactc gatgctgatc acgctgcgca tcaacggcgc gcgcgacatc
    27061 gacgagctgg ccgaagccgc ggcgcagatc accgacatga tccggcgcca gttccgcggc
    27121 ggcacgcgcg ccgagctgat cgcgcgcctg gtgcaggacc tcaacgcgcg cctgttcgac
    27181 cgcgcctggc agctcatcgc gccggccgaa ctggtggccc acagctgcct gttcgtcatg
    27241 ggcagcgaag gccgcggcga acaactgctc aagaccgatc aggacaacgg cctgatcctg
    27301 cgcgacggct acgcgccgcc ggccgatctg gccgagctct gccagcggtt ttccgacgcg
    27361 ctggcgcgct ttggctaccc gccctgcccg ggcggcatca tggtcagcaa cccggcctgg
    27421 cgcatgggcg cgaccgaatt tgcccagacc acgcggcgct ggctggccct gcccgagggc
    27481 gacagcctga tgaacctggc catcttcatg gacgcgcacg cggtcagcgg cgacgccagc
    27541 ctgctggacc aggtgcgcga cagcctgacc cagatggcgg tcgacagcga cgccatgctg
    27601 gcgcgttttg ccggcgccat cgacgccttc ggcggcagca tcggctggtg gaaccgcctg
    27661 ctgggcgggg aagcggcgca gctgctgaac ctcaagaagg aaggcatctt ccccctggtg
    27721 cacggcgtgc gcagcctggc gctggcggcg cgcctgccgg tcaccggcac ggtggcgcgc
    27781 atcgaggcgc tggtgcagcg cggcgaactg agcgccgaca ccgggcgcga gctgaccgac
    27841 agcctgcact tctttctcag cctgcgcctg aaggccgggc tggccgaact cgatcgccag
    27901 cagcgggtga cgggggcggt ggacttcaag agcctcaaca gcctggagcg cgatctgctg
    27961 aaggacgcgc tcgacgcggt caagcgcttc aaggcgcagc tgcgctaccg cttcaggctc
    28021 aatgccctgt gacggccatg agcggcctgc ttgaacgtct gcggcgcggc tggcaacgac
    28081 gccagctcac cgacccgcgg tggcgcttcc tgctggacga gccaccaccg cacgaatggg
    28141 tggcgctgga ctgcgagacc accgggctgg atacccgcac cgacgagatc gtcgccatcg
    28201 gcgccgtgcg catcgtcggc cagcgcatcc tgaccagcga gcggctggag ctgctggtgc
    28261 ggcccgagaa gggcgtgtcg gccgaaagca tccgcgtgca ccgcctgcgc cagcaggacg
    28321 tgcaaaccgg cgtgccggtg gccgaggcca tgcagcgcct gctgcacttc atcggcgcac
    28381 ggccgctggt gggctattac ctggagttcg acgtcgccat gatcaaccgc gcgctcaggg
    28441 ccaccctggg cctgacgctg ccgcaggaaa agatcgaggt ctcgcgcctg tactacgacc
    28501 acaagttccg tcagctgccg ccctaccggc agcacgacca ggccgacatc gacctgcgct
    28561 ttgccaccat catgcgcgag ctgggcctgc ccacgcgcga cgcccacgac gccctgaacg
    28621 acgccgtgat ggccgccctg gccttcatca agctgcgcgt gctgcgcggt gacgccgact
    28681 gagcttcaaa atccatagct gctggcgctt gtcctgattg cgccagaggc cgatagggca
    28741 caaaaaaacc gggtccgaag acccggtttc cgtggccgaa ggcagggtca gtgcgccgac
    28801 gccttggccg cgcccaggcc ggtttcggag cgcacctgct gggctgcgaa ggccgcgcgc
    28861 tcggcggcgg cgttgctgct gcggtcgagc accgagaaca gccacacgcc aacgaagccg
    28921 atggtcatcg agaacagcgc cggcgacgag taggggaacc atgcgctgcc cttggggttg
    28981 cccagcgtgg cttcccacac cgccggcgac accaccgtca gcaccaccga cgagatcagg
    29041 cccagaaagc cgccgatcac cgcgccacgg gtggtgcagt ctttccacag caccgacagc
    29101 agcagcggcg gcaggttggc cgaggccgcc acggcaaagg ccagcgacac catgaaggcg
    29161 atgttctgct tctcgaacac gatgcccagc agcaccgcga tgacacccag cgtcagggtg
    29221 gtgatgcgcg acacgcgcag ctcggcggcg ctgtcggcct tgcccttctt gaacaccgtg
    29281 gcgtacaggt cgtgcgacac cgccgaggca cccgacagcg tcaggccggc caccaccgcc
    29341 aggatggtgg cgaacgccac cgccgagatg aagccgtaga acacgtcgcc gcccacgctc
    29401 ttggccacca gcaccgccgc catgttggcg gtgccggcgc cgcccttgat cacgcccttg
    29461 gcggtgtcgg cgaactcggg gttggtcagc accagggtga tggcgccaaa gccgat
//
LOCUS       DAOQTZ010000020        28914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1917_length_28914_cov_12.994872, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000020 DAOQTZ010000000
VERSION     DAOQTZ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1917_length_28914_cov_12.994872"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKN51_04010"
     CDS             complement(<1..457)
                     /locus_tag="PKN51_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNT84295.1"
                     /translation="MNPLHDHPITRKWPARHPDRLQLYSLPTPNGVKVSIALEELGLP
                     YEAHRVSFDTQDQLSPEFLSLNPNNKIPAILDPDGPGGQPLALWESGAILLYLADKTG
                     RLLPAQAAARWHALQWLMWQMGGLGPMFGQLGFFHKFAGKDFEDKRPRDR"
     gene            545..2017
                     /locus_tag="PKN51_04015"
     CDS             545..2017
                     /locus_tag="PKN51_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT84296.1"
                     /translation="MNAPLHHELPPPLLDAAQARARIDAQWDGDILRQLTDYIAIPAK
                     SPVFDPDWAAHGFIDAVVRQAAGWVEAQKVPGLTLEVVRLPGRTPVIFFEIPATRPGS
                     TQTALLYGHLDKQPEFTGWRADLGPWTPKLEDGKLYGRGGADDGYAVYAAIAAVQALK
                     AQQLPHPRLVGLIETSEESGSPDLLPYIDALRPRLGDVGLVVCLDSGAGNYDQFWLTT
                     SLRGNITGELRVEVLTEGVHSGDASGLVPSSFRILRQVLERLEDSATGRLLPPELHCD
                     VPPERVAQARATAAILGDDLFRRFPWVHYDCGGTAQSVLPMTGDAVDALLARTWRPTL
                     SVTGAEGLPPLREAGNVLRPYTAFKLSLRLPPLVDAAQAVQQLKALLEDNAPYQARVT
                     FDSLGAATGWNAPDAAPWFAQALDVASRAHFNAPCGYIGQGGTIPLMNQLSQGFPTSQ
                     MMVCGVLGPRSNAHGPNEFLHLSYAKRLTAAVAEVIARMP"
     gene            2017..2892
                     /locus_tag="PKN51_04020"
     CDS             2017..2892
                     /locus_tag="PKN51_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNT84297.1"
                     /translation="MRGDSTWSQFVSTHGESLALHDWPLSAGGAARGVVLLVHGLGEH
                     MGRYQHVADRLNQWGFSVRGYDQYGHGESAGVRGDLPSDDRLLTDLAAIVDDTRARMD
                     DRLPLILLGHSMGGLVAARLVSLKLRRVDALVLSSPALDAGLSVVQKALAASLWRLAP
                     RARVANGIDPNGLSRDPAAVQAYQADPLVHDRISARLAHFIASAGPAVLQRAPRWKVP
                     TLLLYAGHDRAVSPAGSEAFARAAPADCVTVRAFPLHYHELFNDLDNDAVFAVLRDWL
                     TTSFAPVALGRAAPF"
     gene            complement(2921..4096)
                     /locus_tag="PKN51_04025"
     CDS             complement(2921..4096)
                     /locus_tag="PKN51_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT84298.1"
                     /translation="MNRHLLLLTLVQGLFLVNNVTFIAINGLVGLQLAPYGWMATLPV
                     MGYVVGGALAAPLVARTQARFGRQGSFQIGLLVALGSALLAAWAAQSRQFGLLLAATV
                     IAGYYNANGQLYRFAAAELVAPHWREKAVSLVLAGGLLGAVVGPNLAAWTRTVLPTPF
                     VGAYLALALVALLAMGVLALIEFPPAPAAAAGADRGRPLGQIMRQPAFIVATVGAALG
                     YGVMNLLMAATPLAMQVCGFEFGATAQVLQWHVIGMYAPGFFTGHLIRRFGALAVMGA
                     GVVLNLLCVAVALTGVELTQFLAALTLLGVGWNFLFTGSTTLALTAYRPEEKDRAQAA
                     INFWVFATMAVTSFASGALVTTQGWTWLNLGSLLPLLVTGAALAWLGLRRRAASAGA"
     gene            complement(4163..5011)
                     /locus_tag="PKN51_04030"
     CDS             complement(4163..5011)
                     /locus_tag="PKN51_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HNT84299.1"
                     /translation="MLLNGFYINLDESPQRHHAMQARLKALGLDGVTQRFPGQRGDGR
                     AARISASQLGCFLSHQAVLEQAPADRFTLVLEDDASLAAALPRRLPALLDPAQAQPWD
                     ILFLGQTVPYDSIHPLDRLIKASKELGDIGAPDFARFFLMDGAMHYVWGTFGYVVHPR
                     ALPRLRALLRQFAAHGYPTSWDGTLRTLIKQGQLTARCLFPYLVGVDTGHDSTISGRG
                     PHLQHQLHALLVNLFLADADEQQRLRAQALAFCPSATDWQAAYVGWLIYRKLSAPADA
                     DTSAFA"
     gene            complement(5050..5763)
                     /locus_tag="PKN51_04035"
     CDS             complement(5050..5763)
                     /locus_tag="PKN51_04035"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNT84300.1"
                     /translation="MPRFHVPLPLAEGAQLALPAGAARHVQVLRLQPGAEVTLFNGAG
                     GEWQARITRMGRSEVEVVVGPHHAAEREAARPVHLAVGLMAAERMDWLVEKATELGAT
                     SLTPLLSARSSLRLSGERAARKRAHWQAVAAAACEQSGRNRLLDVREQLTFQEYLGQV
                     PEGARWLLSLDPAAPPLRQRLAAQAAPDPVILLSGPEGGLEPAEQAAAQRASFAPVTL
                     GPRVLRAETAPLAALAALA"
     gene            5830..6573
                     /locus_tag="PKN51_04040"
     CDS             5830..6573
                     /locus_tag="PKN51_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNT84301.1"
                     /translation="MNAPPTATPCPSCRAPMQPQQVAAHEGATLTLDLCHACHGIWFD
                     PQENQRIAPEGVLGLFRQLHEHREDAQQPLARQLRCPRCRDPLTEGSDLVRSGRYFTW
                     RCPQRHGRFATFSSFMIEKGFVRQLSEAEITALAERTRIIHCGSCGAPVDLRQDRACP
                     YCRAALSLLDPQAVERALDGYHTKAQRQAEGPRAPDLADALITLERDRERARREAQQQ
                     RWQGRSPSSVDELWLAGINLVWQWLSRRG"
     gene            complement(6625..7941)
                     /gene="fahA"
                     /locus_tag="PKN51_04045"
     CDS             complement(6625..7941)
                     /gene="fahA"
                     /locus_tag="PKN51_04045"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNT84302.1"
                     /translation="MTLDHTHDPTARSWLAAANAAGGDFPIQNLPFAVLRRAGSGEPF
                     RGAVAIGDQALDLAALAAAGLVQGAAAEALALAAQPQLNAFMAAGPAAWRALRHALFA
                     LLREGVAIEAAVRACLRPLASVEYALPAHIGDYTDFYTSLDHAVNCMRIMRPDADVTP
                     NFRWLPQAYHGRVSTIGVSGQRFHRPHGQFLPPGASAPVFAPCTAMDYELELALWVGP
                     GNPAGQPIALANAEQHLFGIGLLNDWSARDIQMWEMTPLGPFHAKNFATTVSPWIVTL
                     EALAPFRRPWSRDAADVQPLPYLDAPANRAQGAIDIQLEVALLSEAARAQGRAPARLS
                     GTSFRHQYWSVAQMLTHHTAGGCALQPGDLLGSGTISGPGPGEAGALIELARGGQQPV
                     DVGGGERRGFLQDGDTVILRGWCEKPGAARIGFGECRGTVLPAISE"
     gene            complement(8196..9005)
                     /locus_tag="PKN51_04050"
     CDS             complement(8196..9005)
                     /locus_tag="PKN51_04050"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNT84303.1"
                     /translation="MTRKFIDLSIYLENDVVSDPPAFAPKIQYFNHQNSFEQMAPFFP
                     GLKQSDLPDGEVWAVEMVQLSTHNGTHLDAPYHFHSTMDKALGGKKPAWTIDEVPLEW
                     CFQPGVKLDFRHFGDGYVVTAADVEAELKRIGHELKPLEIVVVNTRAGSRYGHNDYVA
                     AGCGMGYEATMYLLERGVRLTGTDAWSWDAPFVYTAQKYAESKDAGLIWEGHKAGRDI
                     GYCHLEKLHNLEALPADGFYISCFPHKIRGASAGWTRAVAIFDDALMPASA"
     gene            complement(9020..10057)
                     /locus_tag="PKN51_04055"
     CDS             complement(9020..10057)
                     /locus_tag="PKN51_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNT84304.1"
                     /translation="MPANPLRGFAVDRAQIHRIGHDLQRPECILAEPDGTLWSADARG
                     GVMRIAPDGTQRFIGQRADARFGSAAAGSSAELEHKFTTGTLPNGLAFAANGDILISN
                     FGTDRLEVMTRGGDTRVLYDTIDGRPIGKVNFVLRDSKDRIWITVSTRVNPWTQAAAS
                     RVQDGCIAVVERGVLRVVAEGFFFTNEIRLDAREEWLYIVETTGPHISRMRVVEGAQG
                     VALTDREVFGPAHLGGYPDGIAFDAFGNLWCTLVMVDQLIALTPEGERLLLLDDGNPQ
                     ASQALLAHMAAGTVTAEAMLAARGTLAPWMASVTFGGPDLRTVYIGSLMGTSIPYFRS
                     PVAGLPMVHWK"
     gene            complement(10060..11391)
                     /locus_tag="PKN51_04060"
     CDS             complement(10060..11391)
                     /locus_tag="PKN51_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT84305.1"
                     /translation="MSPIAVACLSLLAMLGLIWAGMHVAVVLALVSFVGVWLIRGDMD
                     VAANLLAQASQDAVASHIFGVVPLFVLTGFLVARADVGKDAFEVANQVLRRVRGGLGV
                     ATVASNAVFAAITGISIASAAVFTRVAVPEMLRFGYQPRFAVGVVAGSSVLGMLIPPS
                     LLMILYGFLANQSVGDLFTAGIVPGLLLATVYSVGIVLMATLRPLAVYADGAARAVEP
                     AALLPAGTLVLKLLPSVLLIASVLGGLYAGFFTATEAGATGAAVALAVALLRRKLDWR
                     TLWGVLTETGHVTVSVSFLIICATIYTRMLAMSGLPQFLVEWMTQLGLGPTGFLLIYI
                     GFIILLGMIIDSSSILLIVLPLMVPIAEQFGMNLIWLGVVTVVAVEIGLLTPPFGLSV
                     FVIKSTLSDSRISLGDIFRGTLPFTLMMLAVLLLLVFVPQLSLLLLKTPGG"
     gene            complement(11450..11998)
                     /locus_tag="PKN51_04065"
     CDS             complement(11450..11998)
                     /locus_tag="PKN51_04065"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT84306.1"
                     /translation="MKSEVTDAYGQPLPGRFERLTQALNALGTLWIVALMLLINTDVL
                     GRELLNAPVRGTTELVSLSIVGIVFLQLADTLASGRLTRADVLLDRLKRTTPWLAALL
                     QALYHLVGAGLMAVILWAAWAPLVESIRIQEYVGALGDFTAPVWPVRLIMLVGMVMTL
                     LTFVLLAWLDLRRLRAGLKGRS"
     gene            complement(12381..13448)
                     /locus_tag="PKN51_04070"
     CDS             complement(12381..13448)
                     /locus_tag="PKN51_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HNT84307.1"
                     /translation="MPTRRLIATAALLAAAGLGAPAHAQQNIRLTAAAGHPPVFLWVK
                     LVDEFFIREVDKRLAAAGKHKIEWTRAWGGTLIKLGAESKGIGDGVADLGFVSTIFEA
                     AKFPTQNVSYYAPFGTDDIGVVTQVVNELQGRIPAMGQAWSKNNLVYLGGAALDAYHI
                     WTSFPLKSVDDLKGRKITAPGPAANWIKGTGAVAVAGNLNTYYEDIKSGVSNGVITFA
                     TGAWGAKVHEVAPYITKVNFGAQYAGGLAINKARFDKLPPEVQKVMREVGAEYSQRFA
                     AAQAQTAAALLKKAEEAGAKLSELPAAERKRWADTLPPIAKTFAADLQAKGLPGDQVL
                     HGFMDGLKKAGAQPARDWAGK"
     gene            complement(13452..14144)
                     /locus_tag="PKN51_04075"
     CDS             complement(13452..14144)
                     /locus_tag="PKN51_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNT84308.1"
                     /translation="MRTVLTRPAALAPAADEAARPDRKAAILLAAEKLFAQHGYHAVS
                     IRQIAAEAGVPLALVGYYYGAKHELFHAIFAHWQPTIDQRLALLHQALAGAQPPPLRR
                     VVQAFVEPVLALRASAEGEYYAMLVARELAYRTPDTDRVLAEMFDPMAHAFIDALHQL
                     RPRWPRARAAWAYQFAMGALLHHLMDHRVQRLSHGQNPPHDAAAAPQLIDFITAGIAA
                     VLPTDPTTPQET"
     gene            14252..16357
                     /locus_tag="PKN51_04080"
     CDS             14252..16357
                     /locus_tag="PKN51_04080"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNT84309.1"
                     /translation="MSDAAPTPATAPACIPQIRLYQNWLRDQRGLSFASYRELWRWST
                     TELDAFWQSIWDYADLRSPTPHTAVLGRNVMPGAQWFPGAQVNYAQQVFRHVDAAHAA
                     GQPAVISDDEKGRVRELSWPELRRQVASLALHLQAQGVQPGDRVAAYLPNLPETMVAF
                     LATVSLGAIWSVCAPDMGTHAVLDRFRQIEPKVLIAVDGITWAGRDIDRSAVVRELRA
                     GLPSVQHLLLLRRSDTIETIADGADWTSATARNDAQTAAFEPRWLPMDHPLWIVYSSG
                     TTGLPKPIVHSHGGILLVAIATNLHMDVGSSYGANNFGERFHWYSSTGWVMWNCQVAG
                     LLSGATCVIYDGSPGGPRDAPDWGVLWRFAARHGVTFLGSGAAFFAGCAKAGVDLAQC
                     GDLSRVRALGSTGSPLSAEVQQWGTEQFRAIAKTEAQRDIWWFNISGGTDFAGGFIAG
                     HRELPQRPGVMQCRMLGSAVEAWNDAGEPVLGQVGELVCTQPIPSMPLCFWNDPGNAR
                     YLASYFEMYPPGHGRNPRGGDLDSSAGSVWRHGDWLRVGDEQGQGEWCVIFGRSDATI
                     NRHGLRMGTSEIYSAVEALPEVLDSLVVDLEYLGRDSYMPLFVVLRPGSALDDGLRQR
                     INDAIRQQLSPRFVPDDILQVPEIPRTLSGKKQELPIKKLLLGQPIDKVVNQEAMANP
                     GSLPWYVAFAQQRAQAHTG"
     gene            16729..17160
                     /locus_tag="PKN51_04085"
     CDS             16729..17160
                     /locus_tag="PKN51_04085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNT84310.1"
                     /translation="MIDTPIELLRQNFRQAPFIASLGLELASIGAGECTTRLDVRPDH
                     LQQNGVVHAGVLATLADHTAGAAAFTTLAPGSYPLTVEFKLNLLRPATGPTLRCIARV
                     LKAGARLVVVESEVLADATSPARLCAKATVTLAVLTAGEAG"
     gene            complement(17304..18110)
                     /locus_tag="PKN51_04090"
     CDS             complement(17304..18110)
                     /locus_tag="PKN51_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84311.1"
                     /translation="MPSLSETPSTAPSTGSPSRVRFAACYAARHLGLSMLVALLSAAL
                     VFGLLYPAPYRAMLGVESIFLLVLAVDVVCGPLLTLVLASPSKSQRERWLDFSLIGLI
                     QLLALAYGLHSVWVGRPAVLGFEVERLAVVTANEIEADRLAEAPAGLRRLPWWGVQRV
                     GTRQPKDSADMLRSVELGLSGVSLVMQPGWWLPWQDALPAMQARAKPLAELMARRPEA
                     ADALRRAARDSGLPPEQLRYLPLTSHKTLDWVALLDAQMNLVGWAPVDGF"
     gene            complement(18346..18888)
                     /locus_tag="PKN51_04095"
     CDS             complement(18346..18888)
                     /locus_tag="PKN51_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT84312.1"
                     /translation="MKKLQQGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRITEGL
                     SLAEPAKATIGAEGSASAADLTRVANTWNAQAAGTGANSKFVNSVLITAASGLITVTY
                     NPTSVGVAAAANRITLTPWVRNAAAGAGQSLAAAIAAGNSGAIDWGCASATNASATAA
                     GITVGAAGTVLAKYAPAQCR"
     gene            complement(19472..19876)
                     /locus_tag="PKN51_04100"
     CDS             complement(19472..19876)
                     /locus_tag="PKN51_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HNT84313.1"
                     /translation="MHIACFDDLLRAARAQIEPQRLLFVFTEAELPDAPTPEQRAGFA
                     RGEGGALVPRLCVDKTPDELVSFDALAHQATQLHPAWTLVFAAALGGTLHRAPSSADA
                     QAPLQRMVDAIKAGRHEGLIPFDRQGQAVRLY"
     gene            20121..20966
                     /locus_tag="PKN51_04105"
     CDS             20121..20966
                     /locus_tag="PKN51_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNT84314.1"
                     /translation="MDPSAVTLFRLAVLCALGTAATTAGAQSMVTARVLSAQPVVEQV
                     PVSDCGPYGAPPSGAGAAVGALTGGLIGSQLGKGSGHIAGAVLGALGGAFLGNAAEAT
                     QYGGYGGCATRYSQRVTGYDVVYEVAGRSYQTRMAQAPGQWIQVPAPDAYHGQPAWGA
                     GEVQSYPVAPPSLAGYPMPVYPAAGESSGVVTAPPAWGGQPYPVPYPGGYGPVVPQGY
                     AAPYPPAYAPPAYPVYPAYPPAAYAPPPVRYVAPVGVNLSIGGVIGGRGRGHGHWGVG
                     IGSGW"
     gene            complement(21082..21744)
                     /locus_tag="PKN51_04110"
     CDS             complement(21082..21744)
                     /locus_tag="PKN51_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNT84315.1"
                     /translation="MTLKLYYAPGACSFVPHAMLEIAGATFEPMAIRLHKNEQRNPDY
                     LALNARGQVPVLVDGDAVITQLMAIVAYIDARFPEHAFVPKEPLARARFVEALAWMNN
                     TVHPTFTHIFMPHKFSDDPAAQQSIRAQALTAYRQQLGELQALVQQAHSAGRDWLGGV
                     HCGPLDAYALTLLRWGSMVDINPEDTPALWAHAQRTAQVPGVARAMAREKLPLSMYQP
                     GA"
     gene            complement(21883..22608)
                     /locus_tag="PKN51_04115"
     CDS             complement(21883..22608)
                     /locus_tag="PKN51_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNT84316.1"
                     /translation="MIGRLVLLLLAANLGWLAWTQGWLRPLGLVPQEQAEPQRLQRQV
                     QPQALRLQPLEPASAAAAADDTPARRSTAASATPASAAEPAAASASAPAPTACLQIGT
                     FDASQIEAVRQAAAGLPEGSWRVDAVQLPGRWMVYLGKLPDAAAVAARRAELRAQGVD
                     TDRPGAALEPGLSLGRFASEEAAERALDNLARKGVRGARVVRERRDTPAYLLRLPQAD
                     AALRRQARALNGALGGHDWRPCE"
     gene            complement(22605..23405)
                     /locus_tag="PKN51_04120"
     CDS             complement(22605..23405)
                     /locus_tag="PKN51_04120"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNT84317.1"
                     /translation="MTAPTLSWPVDALRSALTPLLPGLVVEVAGQIDSTNSELMRRAR
                     AGRLPPTLLVAELQTAGRGRLGRGWQSGDGEAGGALAFSLALPLAPHDWSGLSLAVGL
                     AVAEALHPDIRLKWPNDLWWQGRKLAGILVETASGAAAAGAGRCAIVGIGVNIAPLDG
                     TGFATPPAWLQALLPGVDAPGALLRIAVPLVHALHAFEQTGFAPLAPRFATRDLLAGQ
                     PVQLSDGSEGVALGVGPDGALRVRCATGVREVRSAEVSVRPAAPGAPA"
     gene            complement(23402..23944)
                     /locus_tag="PKN51_04125"
     CDS             complement(23402..23944)
                     /locus_tag="PKN51_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNT84318.1"
                     /translation="MANPTPRKTAAPRRIQVRRSGVHGKGVFALVDIPKGTRIIEYVG
                     QVISWDEAQRRHPHDPQDPNHTFYFHIDDGHVIDALYGGNASRWINHSCSPNCKADEQ
                     GGRIFITARRAIRAGEELNYDYGLVIDEPLTDELRADYPCRCGSRACRGTLLSSDLDS
                     AEDQAGRRRSGRKSGRQPPP"
     gene            24052..27003
                     /locus_tag="PKN51_04130"
     CDS             24052..27003
                     /locus_tag="PKN51_04130"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HNT84319.1"
                     /translation="MKTLVIAEKPSVAQDIVRALTPVAGKFDKHDEHFENERYVVTSA
                     VGHLVEIQAPEQFDVKRGKWSFAHLPVIPPHFDLKPVDKTRTRLNAVVRQARRKDVGE
                     LINACDAGREGELIFRLIEQYAGGAKPLGKPVRRLWLQSMTPQAIREGFERLRSDAQM
                     QGLADAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLAVVVEREEQI
                     RRHVARDYWELHASFEAEAGSYVGKWFDPDWKKDPEDAEKRADRLWDEARARAIAAAV
                     QGQPASVREESKPSSQLSPLLFDLTSLQREANGKFGFSAKTTLALAQSLYEKHKALTY
                     PRTDSRHLPEDYVGVARQTFEMLAGGHQTHLAPHASKALAEGYVRPGKRIFDNGKVSD
                     HFAIIPTLQEPGALSEAERKLYDLVVRRFMAVFYPAAQYQVTTRITTVEHGSERHRFQ
                     SNGKVLVQPGWLAIYGKEAEGEQGEDKDGKSLVPVRPGEQVMHRDTEVKALATRPPAR
                     YSEATLLGAMEGAGKWIDDDELREAMQDKGLGTPATRAAIIEGLIGEKYLLREGRELI
                     PTAKAFQLMTLLRGLQVEELSKPALTGDWEYKLAQMERGQLARADFMREIAAMTERIV
                     RKAKEYDRDSVPGDYAQLATPCPQCGGVVRENYRRYACTGAAGAGEGCGFSFTKTPGG
                     RTFATDEAEQFLRQRRIGPLEGFRSKAGWPFVAELALVRDEAAGQWKLEFDFGDDARA
                     EASGEIVDLSDQPSLGACPKCGGAVKAYGKSYLCERSLPSHAQPTPSCDFKSGQVILQ
                     QPVEPEQMRKLLAEGRTDVLDRFVSLRTRRPFKARLAWNADEGKVGFEFEPREGGRKY
                     PPRPSTASKTIAAGAAPAGAVARKSAKKATAAGTAAKTRAPKADKAPKAPRAGNLHPS
                     PELAAVIGPGPFGRGEVRKKLWDYIKAHGLQDPQDKRSIVADDKLRPILGAERVGMFK
                     LAGLAGAHLKA"
     gene            27053..27520
                     /locus_tag="PKN51_04135"
     CDS             27053..27520
                     /locus_tag="PKN51_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT84320.1"
                     /translation="MNITQDTAVTLDYRITDAQGKPLDAGTTAYLHGGYDNLFAKAEA
                     ALQGKTVGDKVALALSAAEAFGARDEALARRIPKSEFPPGVKVGGQLQGAGPHGPVVY
                     RVVKIKGPEVLLDANHPLAGLDIRFAATVKAVRAATAEEIAHRHVHGAHGHHH"
     gene            27649..28896
                     /locus_tag="PKN51_04140"
     CDS             27649..28896
                     /locus_tag="PKN51_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT84321.1"
                     /translation="MAPASDPHAAAPPLAARQAWAMLLALTGAFVLSQAFRTLGAIMG
                     PPLAAQLQLSAQQLGLWSAAFHFSFGAMQLAMGVSLDLWGVRRTILLAFPVAVAGALL
                     SSQADGFGMLLLGQALIGAGCAPAFLVCTVFIGRHFAPERFTAMSGLTMSLSGLGVLA
                     TASPLAWLIERSHWRWGFGVLAGLSVLVWLVIAWRVHEPARPADAGAPRPTALDALRG
                     LLGLFALPQTAGVVAYAAVGYAAFITLRGLWLGPLLVQRFDLSLLQAGHVALAMTLAS
                     MASPAIFGRLDPGGARRLRWLLTLATVAALLLATIGLVPLLWLDVLLAGSYGLISGFG
                     VLQYGYVNDAYPPALRGRAMSLLTMAMFLGVSLMQWASGLAASLAPTLGVEPFSAALL
                     TMAAMLLAGALALWKLPRAPLAQ"
BASE COUNT         4241 a        10240 c        10387 g         4046 t
ORIGIN      
        1 agcggtcgcg cgggcgcttg tcctcgaagt ccttgccggc gaacttgtgg aagaaaccga
       61 gctggccgaa catcggcccc agcccgccca tctgccacat cagccattgc agcgcgtgcc
      121 agcgcgccgc cgcttgcgcc ggcagcaggc ggccggtctt gtcggccagg tagagcaaga
      181 tggcgcccga ctcccacagc gccagcggct ggccgcccgg gccgtcgggg tcgaggatgg
      241 ccgggatctt gttgttcggg ttgagcgaca ggaactcggg cgacagctga tcctgcgtgt
      301 cgaagctgac gcggtgcgcc tcgtagggca ggcccagttc ctccagcgcg atcgacacct
      361 tcaccccgtt gggcgtgggc agcgaataca gctgcagccg atcggggtgg cgcgccggcc
      421 atttgcgggt gatggggtgg tcgtgcaggg ggttcatgcg gggcaatata cgcccgctgc
      481 tggcgtcgcg cggcgctcag gggtaaagtg ccacgctgcc ctttccgttc cgcatcgcac
      541 cgccatgaac gcccccctgc accacgaact gcccccaccc ctgctcgacg ccgcccaggc
      601 gcgcgcgcgc atcgacgccc agtgggacgg cgacatcctg cgccagctca ccgactacat
      661 cgccatcccg gccaagtcgc cggtgttcga cccggactgg gcggcgcacg gcttcatcga
      721 cgccgtggtg cgccaggccg ccggctgggt cgaggcgcag aaggtgcccg gcctgacact
      781 ggaggtggtg cgcctgcccg ggcgcacgcc ggtgatcttt ttcgagatcc cggccacccg
      841 acccggcagc acgcagaccg cgctgctgta cggccacctc gacaagcagc ccgaattcac
      901 cggctggcgc gccgacctgg ggccgtggac gcccaagctg gaagacggca agctgtacgg
      961 ccgcggcggc gccgacgacg gctatgcggt gtacgccgcc atcgccgccg tgcaggcgct
     1021 gaaggcgcag cagttgccgc acccgcgcct ggtcggcctg atcgagacca gcgaagaaag
     1081 cggctcgccc gacctgctgc cttacatcga cgccctgcgc ccgcgcctgg gcgacgtcgg
     1141 cctggtggtg tgcctggact cgggcgccgg caactacgac cagttctggc tcaccaccag
     1201 cctgcgcggc aacatcaccg gcgagctgcg cgtcgaggtg ctgaccgaag gcgtgcactc
     1261 gggcgacgcc tccggcctgg tgcccagcag cttccgcatc ctgcgccagg tgctggagcg
     1321 gctggaggat tcggccaccg gccgcctgct gccgcccgag ctgcactgcg acgttccgcc
     1381 cgagcgcgtg gcgcaggcgc gcgccaccgc cgccatcctg ggcgacgacc tgttcaggcg
     1441 cttcccctgg gtgcactacg actgcggcgg caccgcccag tcggtgctgc cgatgaccgg
     1501 cgacgccgtc gacgccctgc tggcgcgcac ctggcgcccc acgctcagcg tcaccggcgc
     1561 cgagggcctg ccgccgctgc gcgaggccgg caacgtgctg cgcccctaca ccgccttcaa
     1621 gctcagcctg cgcctgccgc cgctggtgga cgcggcgcag gcggtgcagc agctcaaggc
     1681 gctgctcgaa gacaacgccc cctaccaggc gcgcgtcacc ttcgacagcc tgggcgccgc
     1741 caccggctgg aacgcgcccg acgccgcgcc ctggttcgcc caggcgctgg acgtggcctc
     1801 gcgcgcccac ttcaacgccc cctgcggcta catcggccag ggcggcacca tcccgctgat
     1861 gaaccagctg agccagggct ttccgacctc gcagatgatg gtctgcggcg tgctcggccc
     1921 gcgcagcaac gcccacgggc ccaacgagtt cctgcacctg tcctacgcca agcgcctgac
     1981 cgccgccgtg gccgaagtca tcgcccggat gccctgatgc gcggcgactc cacctggtcg
     2041 cagttcgtca gcacgcacgg cgagtcgctg gcgctgcacg actggccgct gtcggccggc
     2101 ggcgcggcgc gcggcgtggt gctgctggtg cacggcctgg gcgaacacat gggccgctac
     2161 cagcatgtgg ccgaccgcct caaccagtgg ggcttttcgg tgcgcggcta cgaccagtac
     2221 ggccatggcg aatcggccgg cgtgcgcggc gacctgccca gcgacgaccg cctgctgacc
     2281 gacctggcgg ccatcgtcga cgacacccgc gcccgcatgg acgaccggct gccgctgatc
     2341 ctgctcggcc attcgatggg tggcctggtg gcggcgcggc tggtgtcgct caagctgcgc
     2401 cgggtcgacg cgctggtgct gtcctccccc gcgctggacg ccggcctgag cgtggtgcag
     2461 aaggcgctgg cggcctcgct gtggcggctg gcgccgcgcg cacgggtggc caacggcatc
     2521 gacccgaacg gcctgtcgcg cgacccggcg gcggtgcagg cctaccaggc cgacccgctg
     2581 gtgcacgacc gcatcagcgc ccggctggcg catttcatcg ccagcgccgg gccggccgtg
     2641 ctgcagcgcg cgccgcgctg gaaagtgccc accctgctgc tgtacgccgg ccacgaccgc
     2701 gccgtctcgc cagccggcag cgaagccttt gcccgcgccg cgcccgccga ctgcgtgacc
     2761 gtgcgcgcct tcccgctgca ctaccacgaa ctgttcaacg acctcgacaa cgacgccgtg
     2821 ttcgccgtgc tgcgcgactg gttgaccacc agctttgccc cggtggcgct ggggcgcgcg
     2881 gcgccgttct gaggcgcccg ggccggcccg gtgtcgggct tcaggcgcct gccgaggccg
     2941 cgcgccggcg caggcccagc caggccaggg cggcgccggt caccagcagc ggcagcagcg
     3001 agcccaggtt cagccaggtc cagccctggg tggtcaccag cgcgcccgag gcgaacgagg
     3061 tgacggccat ggtggcgaac acccagaagt tgatcgccgc ctgcgcacgg tccttttctt
     3121 ccggccggta ggcggtcaaa gccagcgtgg tgctgccggt gaacaggaag ttccagccca
     3181 cgcccagcag cgtcagcgcg gccaggaact gcgtcagctc caccccggtc agcgccaccg
     3241 ccacgcacag caggttcagc accacgccgg cccccatcac cgccagcgcg ccgaagcgcc
     3301 ggatcaggtg gccggtgaag aaacccggcg cgtacatgcc gatcacgtgc cattgcagca
     3361 cctgcgccgt ggcgccgaac tcgaagccgc acacctgcat ggccagcggc gtggcggcca
     3421 tcagcaggtt catcacgcca tagcccagcg ccgcgcccac ggtggcgacg atgaaggccg
     3481 gctggcgcat gatctgcccc agcggccggc cacggtcggc cccggcggcc gccgccggcg
     3541 ccggcggaaa ctcgatcagc gccagcaccc ccatggccag cagcgccacc agcgccagcg
     3601 ccaggtaggc gccgacgaag ggcgtgggca gcacggtgcg cgtccaggcc gccaggttgg
     3661 ggcccaccac cgcgcccagc aggccgccgg ccagcaccag cgacaccgcc ttctcgcgcc
     3721 agtgcggcgc caccagctcg gcggcggcaa agcggtacag ctggccgttg gcgttgtagt
     3781 agccggcgat caccgtggcc gccagcagca gcccgaactg gcgcgactgc gccgcccagg
     3841 ccgccagcag ggccgagccc agcgccacca gcaggccgat ctggaacgaa ccctggcgcc
     3901 cgaagcgcgc ctgcgtgcgc gccaccagcg gcgccgccag cgccccgccc accacgtagc
     3961 ccatcaccgg cagcgtggcc atccagccat agggcgccag ctgcagcccg accagcccgt
     4021 tgatggcgat gaaggtcacg ttgttgacca gaaacaggcc ctggaccagg gtgagcagca
     4081 gcaggtggcg gttcatgggg tcgtcagaat actattgaaa caggagcttg cgacgcttga
     4141 ttctggcggc atggcaagga tttcaggcga aagcgctggt gtctgcgtca gcgggtgccg
     4201 acagtttgcg gtagatcagc cagccgacgt acgcggcctg ccagtcggtg gccgaggggc
     4261 agaaggccag ggcctgcgcg cgcagccgct gctgttcgtc ggcatcggcc aggaacaggt
     4321 tgaccagcag ggcgtgcagc tggtgctgca gatgggggcc gcggccgctg atggtgctgt
     4381 cgtgtccggt gtcgacgccg accaggtacg ggaacaggca gcgggccgtc agctggccct
     4441 gcttgatcag ggtgcggagg gtgccgtccc acgaggtcgg gtagccgtgg gccgcgaact
     4501 gccgcagcaa ggcgcgcagc cgcggcagcg cccgggggtg gacgacgtag ccgaacgtgc
     4561 cccagacgta gtgcatcgcc ccgtccatca ggaaaaagcg cgcaaagtcg ggcgcgccga
     4621 tgtcgcccag ttccttgctg gctttgatca ggcggtccag agggtgaatg ctgtcgtagg
     4681 gcacggtctg gcccaggaac aggatgtccc agggctgcgc ctgtgccggg tccagcagcg
     4741 ccggcaggcg ccgcggcagg gcggcggcca ggctggcgtc gtcctcgagc accagggtga
     4801 agcggtctgc gggggcctgc tccagcaccg cctggtgcga cagaaagcag cccagttgcg
     4861 aggccgagat gcgggccgcg cggccatcgc cgcgctgccc cggaaagcgc tgcgtcacac
     4921 cgtccagccc cagcgccttc aggcgcgcct gcatggcgtg atggcgctgc ggcgattcgt
     4981 ccaggttgat gtagaagccg ttgagcagca tggtgatggc cggtggatgc aagaccgctg
     5041 gcgccggctt caggccagcg ccgccagcgc cgccagcggc gcggtttcgg cgcgcagcac
     5101 gcgcgggccg agggtgacgg gggcaaagct ggcgcgctgc gcggcggcct gttcggccgg
     5161 ctccagcccg ccttcggggc cggacagcag gatcaccggg tcgggcgcgg cctgcgcggc
     5221 caggcgctgg cgcagcggcg gcgccgccgg atcgagcgac agcagccagc gcgcgccttc
     5281 cggcacctgc cccagatact cctgaaatgt gagctgttcg cgcacgtcca acaagcgatt
     5341 gcgcccactt tgttcgcagg cggcggcggc cacggcctgc cagtgggcgc gcttgcgggc
     5401 ggcgcgctcg ccgctcaggc gcaggctgct gcgcgcgctc agcagcggcg tcaggctggt
     5461 ggcgcccagc tcggtggctt tttcgaccag ccagtccatg cgctcggccg ccatcaggcc
     5521 gaccgccaga tgcaccgggc gcgcggcctc gcgttcggcc gcgtggtgcg ggccgaccac
     5581 cacctcgacc tcgctgcgcc ccatgcgggt gatgcgggcc tgccactcgc cgccggcgcc
     5641 gttgaacagc gtgacttcgg cgcccggctg caggcgcagc acctgcacat ggcgcgccgc
     5701 tccggcgggc agcgccagct gcgcaccttc ggccaggggc agggggacgt gaaagcgtgg
     5761 catggcgtac aggcgaatcg gcagcggccg atggtacgcc gaccgccgca tgccgtagca
     5821 tcggtgccca tgaatgcccc gcccacggcc acgccctgcc cgtcctgccg cgcgcccatg
     5881 cagccgcagc aggtggcggc gcacgagggc gcgacgctga cgctggatct gtgccacgcc
     5941 tgccacggca tctggttcga tccgcaggaa aaccagcgca tcgcacccga aggcgtgctg
     6001 gggctgtttc gccagctgca cgagcaccgc gaagacgccc agcagccgct ggcgcgccag
     6061 ctgcgctgcc cgcgctgccg cgacccgctg accgaaggtt cggacctggt gcgcagcggg
     6121 cgctatttca cctggcgctg tccgcagcgg cacggccgtt ttgccacctt ctcgtcgttc
     6181 atgatcgaaa agggcttcgt gcgccagctc agcgaggccg aaatcaccgc gctggccgag
     6241 cgcacgcgca tcatccactg cggcagctgc ggcgccccgg tcgatctgcg ccaggaccgc
     6301 gcctgcccgt actgccgcgc cgcgctgtcg ctgctggatc cgcaggcggt cgagcgggcg
     6361 ctggacggct accacaccaa ggcgcagcgc caggccgaag gcccgcgcgc gcccgacctg
     6421 gccgacgcgc tgatcacgct ggagcgcgac cgcgaacgcg cccggcgcga ggcccagcag
     6481 cagcgctggc agggccgctc gcccagcagc gtcgacgagc tgtggctggc cggcatcaac
     6541 ctggtctggc aatggctgag ccgccgcggt tagtatcaaa acaatagctg ctggcgtttg
     6601 ctgcaagcgc gccacaggca ctttttattc agaaatcgcg ggcagcacgg tgccccggca
     6661 ctcgccaaag ccgatgcgcg ccgcgcccgg cttttcgcac cagccgcgca ggatcacggt
     6721 gtcgccatcc tgcagaaagc cgcgccgctc gccgccgccc acgtccaccg gctgctggcc
     6781 gccgcgcgcc agctcgatca gcgcgccggc ctcgcccggc ccgggccccg agatggtgcc
     6841 gctgcccagc aggtcgcccg gctgcagggc gcagccgccg gccgtgtggt gcgtgagcat
     6901 ctgcgccacg ctccagtact ggtggcgaaa gctggtgccc gacagccgtg ccggcgcgcg
     6961 cccctgcgcc cgcgcggctt cgctgagcag cgccacttcc agctggatgt cgatcgcgcc
     7021 ctgcgcccgg ttggcgggcg catccaggta gggcagcggc tgcacgtcgg ccgcatcgcg
     7081 cgaccagggc cggcggaagg gcgccagcgc ctccagcgtg acgatccagg gcgacaccgt
     7141 ggtggcgaag ttcttggcgt ggaacggccc cagcggcgtc atctcccaca tctggatgtc
     7201 gcgcgccgac cagtcgttga gcaggccgat gccgaacagg tgctgctcgg cgttggccaa
     7261 ggcgatgggc tggccggccg ggttgccggg gccgacccac agcgccagct ccagctcgta
     7321 gtccatggcc gtgcagggcg cgaacaccgg cgcgctggcg cccggcggca gaaactgccc
     7381 gtgcgggcga tgaaagcgct ggccgctcac accgatggtc gacacgcgtc cgtggtaggc
     7441 ctgcggcagc cagcggaagt tgggcgtcac gtcggcgtcc ggccgcatga tgcgcatgca
     7501 gttcaccgcg tggtcgagcg aggtgtagaa gtcggtgtag tcgccgatgt gcgctggcag
     7561 cgcgtattcg acgctggcca gcggccgcag gcaggcgcgc acggcggcct caattgcgac
     7621 gccttcgcgc agcagcgcaa acagggcgtg gcgcagcgcc cgccaggccg cggggccggc
     7681 ggccatgaag gcgttgagct ggggttgggc ggccagcgcc agcgcctcgg ccgccgcgcc
     7741 ctgcaccagg ccggcggcgg ccagcgccgc caggtccagc gcctgatcgc cgatcgccac
     7801 cgcgccgcgg aacggctcgc cgctgccggc gcggcgcagc acggcaaagg gcaggttctg
     7861 gatcgggaag tcgccgcccg ccgcgttggc ggcggccagc cagctgcgcg cggtgggatc
     7921 gtgggtgtgg tcaagtgtca tgattcaatc ctgaattcat agctgccggc gcttgatggg
     7981 taagcgctgg agcatgtttt gcctgattgg gtgcttatct cggtgcctct gcgcctgact
     8041 ccctcccctt ctgggggagg gctggggtgg gggccagcgg cgtgggttca catgaagcgc
     8101 cagtgatgtg ccagtggccc ccatccctgc cttcccccag agggggaagg agtctgagac
     8161 atgtcgctga tcaagtgttt ttgcgatgga tgcggtcagg cactggccgg catcagcgca
     8221 tcgtcaaaga tcgccaccgc ccgcgtccag ccggccgagg cgccgcggat cttgtgcgga
     8281 aagcagctga tgtaaaagcc gtcggccggc agcgcctcca ggttgtgcag cttttccagg
     8341 tggcagtagc cgatgtcgcg cccggccttg tggccttccc agatcaggcc ggcgtccttg
     8401 gattcggcgt acttctgcgc ggtgtagacg aagggcgcgt cccagctcca ggcatcggtg
     8461 ccggtcaggc gcacgccgcg ctccagcaga tacatcgttg cctcgtagcc catgccgcag
     8521 ccggcggcca cgtagtcgtt gtgaccgtag cggctgccgg cgcgggtgtt gacgaccacg
     8581 atctccagcg gcttgagttc gtggccgatg cgcttcagct cggcctcgac gtcggcggcc
     8641 gtcaccacgt agccgtcacc gaagtggcga aagtccagct tgacgcccgg ctgaaagcac
     8701 cactccagcg gcacctcgtc gatggtccag gccggcttct tgccgcccag cgccttgtcc
     8761 atggtgctgt gaaaatggta gggcgcgtcc aggtgcgtgc cgttgtgggt cgacagctgc
     8821 accatctcca ccgcccacac ctcgccgtcg ggcaggtcgg attgcttgag gcccggaaag
     8881 aacggcgcca tctgctcgaa cgagttctgg tggttgaagt actggatctt gggcgcgaag
     8941 gccggcgggt cggagacgac gtcgttttcc aggtagatcg agaggtcgat gaacttgcgg
     9001 gtcatgaggg gctactcctt tacttccagt gaaccatggg caggccggcc acgggcgagc
     9061 ggaaatacgg gatgctcgtg cccatcaggc tgccgatgta gacggtgcgc aggtcgggtc
     9121 cgccaaaggt gacgctggcc atccagggcg ccagcgtgcc gcgcgcggcc agcatggcct
     9181 cggccgtcac ggtgccggcg gccatgtgcg ccagcagcgc ctgcgatgcc tgcgggttgc
     9241 cgtcgtccag cagcagcagg cgttcgccct cgggcgtcag cgcgatcagc tgatcgacca
     9301 tcaccagcgt gcaccacagg ttgccgaagg cgtcgaaggc gatgccgtcc ggatagccgc
     9361 ccaggtgcgc ggggccaaac acctcgcggt cggtcagcgc cacaccctgc gcgccctcga
     9421 cgacacgcat gcggctgatg tgcgggccgg tggtctcgac gatgtagagc cactcctcgc
     9481 gcgcatcgag ccggatctcg ttggtgaaga aaaagccctc ggccaccacg cgcagcacgc
     9541 cgcgctcgac caccgcgatg cagccgtcct gcacgcgcga ggcggccgcc tgcgtccagg
     9601 ggttgacgcg ggtggagacg gtgatccaga tgcggtcctt gctgtcgcgc agcacgaagt
     9661 tgaccttgcc gatcggccgg ccgtcgatgg tgtcgtagag cacgcgggta tcgccacccc
     9721 gtgtcatcac ttccagccgg tcggtgccga agttgctgat caggatgtcg ccattggcgg
     9781 caaaggccag gccgttgggc agggtgccgg tggtgaactt gtgctccagc tcggcgctgc
     9841 tgcccgccgc cgcgctgcca aagcgcgcgt cggcgcgctg gccgatgaag cgctgcgtgc
     9901 catcgggcgc gatgcgcatc acgccgccgc gcgcatcggc cgaccacagc gtgccgtccg
     9961 gctcggccag gatgcattcg gggcgctgca gatcgtggcc gatgcggtgg atttgcgcgc
    10021 ggtcgacggc aaagccgcgc agcgggttgg cgggcatggt catcctccgg gggtcttgag
    10081 cagcagcaga ctgagttgcg gcacgaacac cagcagcagc agcaccgcca gcatcatcag
    10141 cgtgaacggc agggtgccgc ggaagatgtc gcccagcgaa atgcggctgt cggacagggt
    10201 gcttttgatg acgaacaccg acaggccgaa cggcggcgtc agcaggccga tttccaccgc
    10261 caccacggtc accacgccga gccagatcag gttcatgccg aactgctcgg cgatcggcac
    10321 catcagcggc agcacgatca gcaggatcga cgacgaatcg atgatcatgc ccagcaggat
    10381 gatgaagccg atgtagatca gcagaaagcc ggtcggcccc agccccagct gcgtcatcca
    10441 ttcgaccagg aactgcggca gccccgacat cgccagcatg cgcgtgtaga tggtggcgca
    10501 gatgatcaga aagctcaccg acaccgtgac gtggccggtc tcggtcagca ccccccacag
    10561 cgtgcgccag tccagcttgc ggcgcagcag cgccaccgcc agcgccaccg ccgcgccggt
    10621 ggcgccggct tcggtggcgg tgaaaaagcc cgcgtacagc ccgcccagca ccgaggcgat
    10681 cagcagcacg ctgggcagca gcttcagcac cagcgtgccg gccggcagca gcgccgccgg
    10741 ctcgaccgcg cgcgccgccc cgtccgcgta caccgcgagc ggccgcaacg tggccatcag
    10801 cacgatgccg accgaataca ccgtggccag cagcagcccc ggcacgatgc cggcggtgaa
    10861 cagatcgccc accgactggt tggccagaaa gccgtacagg atcatcagca ggctgggcgg
    10921 aatcagcatg cccagcaccg acgagccggc caccaccccg acggcaaagc gcggctggta
    10981 gccgaagcgc agcatctcgg gcaccgccac gcgcgtgaac accgcggccg aggcgatcga
    11041 gatgccggtg atggcggcaa acaccgcgtt cgacgccacg gtggccacgc ccagcccgcc
    11101 gcgcacccgc cgcagcacct ggttggccac ctcgaaggcg tccttgccga cgtcggcgcg
    11161 cgccaccaga aagccggtca gcacgaacag cggcaccacg ccgaagatgt ggctggccac
    11221 cgcgtcctgg ctggcctgcg ccagcaggtt ggccgccacg tccatgtcgc cgcggatcag
    11281 ccacacgccg acgaacgaca ccagcgccag caccaccgcc acatgcatgc cggcccagat
    11341 cagccccagc atcgccagca gcgacaggca ggccaccgcg atcgggctca tggcgcggcc
    11401 ctccgggcgg cgcgcggcgg cgttcgcgct tgcaagcggc ccggcgcgct catgaccgac
    11461 ctttcagccc agcgcgcagg cgccgcagat ccagccaggc cagcagcacg aaggtcagca
    11521 gggtcatcac catgcccacc agcatgatca gccgcaccgg ccacaccggc gccgtgaagt
    11581 cgcccagcgc gcccacgtac tcctggatgc ggatcgactc caccagcggc gcccacgccg
    11641 cccacaggat caccgccatc agccccgccc ccaccaggtg gtagagcgcc tgcagcagcg
    11701 ccgccagcca gggcgtggtg cgcttgaggc ggtccagcag cacatcggcg cgtgtcaggc
    11761 gcccgctggc cagggtgtcg gccagctgca ggaacacgat gccgacgatc gacagcgaca
    11821 ccagttcggt ggtgccgcgc accggcgcgt tcagcagctc gcgccccagc acgtcggtgt
    11881 tgatcagcag catcagcgcc acgatccaca gcgtgcccag cgcgttgagc gcctgcgtca
    11941 gccgctcgaa gcgccccggc agcggctgcc cgtaggcatc ggttacttca gatttcatag
    12001 ctgttggcgc ttgctggaca agcgctggag gaaaaaacgc ttgaaacttt cgaacagccc
    12061 cgggcgcgtg ctaccgagcc ggcctgcttc aagcggccgc gcagccggcc atgccagcgg
    12121 ccgtcgtggc cggggttgcc ccggcggtag gtatcgatcg cttcagattt catagctatt
    12181 ggcgcatgct ggacaagcgc tggaggcata agtcgtttga agtcctcgcc ggcgcccaag
    12241 cgcacgcgac gcgccgccag ccggattcga gcggccgcgg agccggccat gccagcggcc
    12301 gccgtggtcg gggtctcccc ggccactggc ggcgtccccc cgccgggggg aaggcgccgc
    12361 aggcgactca gcggggagcc tcacttcccc gcccagtcgc gcgccggctg cgcgccggcc
    12421 ttcttcagcc cgtccatgaa gccgtgcagc acctggtcgc ccggcagccc cttggcctgc
    12481 aggtcggcgg caaaggtctt ggcgatcggc ggcagcgtgt cggcccagcg cttgcgctcg
    12541 gccgccggca gctcgctcag cttggcgccg gcttcctcgg ccttcttcag cagcgcggcg
    12601 gcggtctgcg cctgggcggc ggcaaagcgc tggctgtact cggcgccgac ttcgcgcatc
    12661 accttctgca cctcgggcgg cagcttgtcg aagcgcgcct tgttgatcgc cagcccgccg
    12721 gcgtactggg cgccgaagtt gaccttggtg atgtagggcg ccacctcgtg caccttggca
    12781 ccccaggcgc cggtggcgaa ggtgatgacg ccgttggaca cgcccgactt gatgtcctcg
    12841 taataggtgt tcaggttgcc cgccaccgcc accgcgccgg tgcccttgat ccagttggcc
    12901 gccgggccgg gggcggtgat cttgcggccc ttgaggtcgt ccaccgactt cagcggaaag
    12961 ctggtccaga tgtggtaggc gtccagcgcc gcgccgccca ggtagaccag gttgttcttg
    13021 ctccaggcct ggcccatggc cggaatgcgg ccctgcagct cgttgaccac ctgggtcacc
    13081 acgccgatgt cgtcggtgcc gaagggcgcg tagtagctca cgttctgggt cgggaacttg
    13141 gccgcctcga agatggtgct gacgaagccc aggtcggcca cgccgtcgcc gatgcccttg
    13201 gactcggcgc ccagcttgat cagcgtgccg ccccaggcgc gcgtccactc gatcttgtgc
    13261 ttgccggcgg cggccaggcg cttgtccact tcgcggatga agaactcgtc gaccagcttg
    13321 acccacagga acaccggcgg gtggccggcc gccgccgtca ggcggatgtt ttgctgggcg
    13381 tgggcggggg cgcccaggcc ggcggcggcc agcagggcgg cggtggcgat caggcggcgg
    13441 gtcggcatgg ctcaagtctc ctgtggggtg gtggggtcgg tgggcagcac ggcggcaatg
    13501 ccggcggtga tgaaatcgat cagctgcggc gcggcggccg cgtcgtgggg cgggttctgg
    13561 ccatgcgaca ggcgctgcac gcggtggtcc atcaggtggt gcagcagcgc gcccatggcg
    13621 aactggtagg cccaggcggc gcgcgcgcgc ggccagcgcg ggcgcagctg gtgcagcgcg
    13681 tcgatgaagg cgtgcgccat ggggtcgaac atttcggcca gcacgcggtc ggtgtcgggc
    13741 gtgcggtagg ccagctcgcg cgccaccagc atggcgtagt actcgccctc ggcgctggcg
    13801 cgcagggcca gcaccggctc cacgaaggcc tgcaccacgc gccgcagcgg cggcggctgc
    13861 gcgcccgcca gcgcctggtg cagcagcgcc agccgctggt cgatggtggg ctgccagtgc
    13921 gcgaagatgg cgtgaaacag ctcgtgcttg gcgccgtagt aatagcccac cagggccagc
    13981 ggcacgccgg cctcggcggc gatctggcgg atcgacaccg cgtggtagcc gtgctgggca
    14041 aacagcttct cggccgccag caggatggcc gccttgcggt cggggcgcgc cgcctcgtcg
    14101 gcggccggcg ccagcgcggc gggacgggtc agcacggtgc gcatggggcg attggacgat
    14161 cgtccaaacc ccttgaacaa ccgtacaaac cctaggccga aagccgcttt cagccgctgc
    14221 gcgcggggcg cggcttgcca ataatcggcc catgtccgac gccgccccca cccccgccac
    14281 cgcccccgcc tgcatcccgc agatccgcct gtaccagaac tggctgcgcg atcagcgcgg
    14341 cctgtcgttt gccagctacc gcgagctgtg gcgctggtcc accaccgagc tcgacgcctt
    14401 ctggcaaagc atctgggact acgccgacct gcgctcgccc acgccgcaca cggcggtgct
    14461 ggggcgcaac gtgatgcccg gcgcccagtg gtttccgggc gcccaggtca actacgcgca
    14521 gcaggtgttt cgccacgtcg atgccgcgca cgccgccggc cagccggccg tcatcagcga
    14581 cgacgaaaag ggccgcgtgc gcgagctgag ctggcccgag ctgcgccgcc aggtggcgtc
    14641 gctggcgctg cacctgcagg cccagggcgt gcagccgggc gaccgcgtgg ccgcctacct
    14701 gcccaacctg cccgaaacca tggtcgcctt tctggccacc gtcagcctcg gcgccatctg
    14761 gagcgtgtgc gcgcccgaca tgggcaccca tgccgtgctc gaccgcttca ggcagatcga
    14821 gcccaaggtg ctgatcgccg tggacggcat cacctgggcc gggcgcgaca tcgaccgcag
    14881 cgccgtcgtg cgcgagctgc gcgccggcct gcccagcgtg cagcacctgc tgctgctgcg
    14941 ccgctccgac actatcgaaa caatagctga cggcgctgac tggacaagcg ccacagcccg
    15001 aaatgacgca caaaccgcgg ccttcgagcc gcgctggctg ccgatggacc acccgctgtg
    15061 gatcgtctac tccagcggca ccaccggcct gcccaagccc atcgtgcact cgcacggcgg
    15121 catcctgctg gtggccatcg ccaccaacct gcacatggac gtcggcagca gctacggcgc
    15181 caacaacttc ggcgagcgct ttcactggta cagctccacc ggctgggtga tgtggaactg
    15241 ccaggtggcc ggcctgctgt cgggcgccac ctgcgtgatc tacgacggca gcccgggcgg
    15301 cccgcgcgac gcgcccgact ggggcgtgct gtggcgcttt gcggcgcgcc acggcgtcac
    15361 ctttctgggc tccggcgcgg ccttctttgc cggctgcgcc aaggccggcg tcgatctggc
    15421 ccagtgcggc gacctgtcgc gcgtgcgcgc cctgggcagc accggctcgc cgctgtcggc
    15481 cgaagtgcag caatggggca ccgagcagtt ccgcgccatt gccaaaacag aggcgcagcg
    15541 cgacatctgg tggttcaata tttcgggcgg caccgacttt gccggtggct tcatcgccgg
    15601 ccaccgcgaa ctgccgcagc gccccggcgt catgcaatgc cgcatgctcg gctcggccgt
    15661 cgaagcctgg aacgacgccg gcgaacccgt gctgggccag gtgggcgaat tggtctgcac
    15721 ccagcccatc ccgtccatgc ccctgtgctt ctggaacgac cccggcaacg cccgctatct
    15781 ggccagctac ttcgagatgt acccgcccgg ccacggccgc aatccgcgtg gcggtgacct
    15841 ggacagcagc gccggcagcg tctggcgcca cggcgactgg ctgcgcgtgg gcgacgagca
    15901 gggccagggc gagtggtgcg tcatctttgg ccgcagtgac gccaccatca accgccacgg
    15961 cctgcgcatg ggcaccagcg aaatctacag cgccgtcgaa gccctgcccg aagtgctcga
    16021 cagcctggtg gtcgatctgg aatacctggg ccgcgacagc tacatgccgc tgttcgtcgt
    16081 gctgcgcccc ggcagcgcgc tggacgatgg cctcaggcag cgcatcaacg acgccatccg
    16141 ccagcagctc agcccgcgct tcgtgcccga cgacatcctg caggtgcccg aaatcccgcg
    16201 caccctgtcg ggcaagaagc aggagctgcc gatcaagaaa ctgctgctcg gccagcccat
    16261 cgacaaggtg gtcaaccagg aggccatggc caaccccggc tcgctgccct ggtacgtggc
    16321 gtttgcgcag cagcgcgccc aggcgcacac cggctgaacg cgtcgcggcc accgcaggcg
    16381 caggcaccag cggccggcgc aggcatcgga tggcgcggcc gcgcgcccat cgtcgcgcct
    16441 tgtccctacc gcctgtcgat gcggccttgg gatgtcctca acaggggcca cgctgacggc
    16501 ccgccgcgtg cccgaccttt gatccagcgc agcccgccgc cggccccggt gcggcacaaa
    16561 ggcgcctgcg cggggcgggc gctgcggcgc gccccatgac ccatgacgcc ggctgcgccg
    16621 cgcatgacga atatcggccg ggccatccgg catgcgtcat cgacgtgcag cgagggcatc
    16681 cgtcgtccgt cacgcgccag cgccacccca accacccgca ggccccccat gatcgacacc
    16741 cccatcgaac tgctgcgcca gaacttccgc caggcgccct tcatagccag tctggggctg
    16801 gagctggcgt ccatcggcgc cggcgaatgc accacccggc tcgacgtgcg ccccgaccac
    16861 ctgcagcaga acggcgtggt gcatgccggc gtgctggcca ccctggccga ccacacggcc
    16921 ggcgcggcgg cgttcaccac gctggcgccc ggcagctacc cgctcacggt ggaattcaag
    16981 ctcaacctgc tgcgccccgc caccggcccc acactgcgct gcatcgcccg ggtgctgaag
    17041 gccggcgccc ggctggtggt ggtcgaatcc gaagtgctgg ccgacgccac ctcaccggcg
    17101 cgcctctgcg ccaaggccac cgtcaccctg gccgtgctga cggcgggcga ggcgggctga
    17161 gcgtggcccg ccagcgatgg cggatgacgc atgaccgcgc tgcgcttcga tcacgcatga
    17221 cgcgcgctcc cgccgcgtgt cggtgaccca ggcgccacgc gtcatgcggg gtggccggcg
    17281 catggggcgt cgcgctatgg cgttcagaac ccgtccaccg gcgcccagcc gaccaggttc
    17341 atctgcgcgt ccagcagcgc cacccaatcc agcgtcttgt ggctggtcag cggcaggtag
    17401 cgcagctgct ccggcggcag gccgctgtcg cgggcggcac ggcgcagggc gtcggcggct
    17461 tcggggcggc gcgccatcag ctcggcgagc ggcttggcgc gcgcctgcat ggccggcagc
    17521 gcgtcctgcc agggcagcca ccagccgggc tgcatcacca gcgaaacccc ggacaggccc
    17581 agttccacgc ttctcagcat gtcggcgctg tccttcggct ggcgcgtgcc cacgcgctgc
    17641 acgccccacc agggcaggcg gcgcaggccg gccggcgctt cggccaaccg gtcggcttcg
    17701 atctcgttgg ccgtcaccac ggccagccgc tcgacctcga agcccagcac cgccggccga
    17761 cccacccaaa cgctgtgcag gccataggcc agtgccagca gctggatcag accgatcagg
    17821 ctgaagtcca gccagcgctc gcgctgcgac ttgctggggc tggccagcac cagcgtcagc
    17881 agcggcccgc acaccacgtc gaccgccagc accagcagaa agatcgactc cacccccagc
    17941 atggcccggt acggcgccgg gtacagcagg ccaaacacca gcgccgccga cagcagggcc
    18001 accagcatcg acaggcccag gtggcgggct gcatagcacg ctgcaaaccg cacgcgtgag
    18061 gggctgccag tcgatggtgc ggtcgatggg gtttcggaga gggagggcat gagcgcgggc
    18121 cgggaaggca acagatcctc gacatccagt gatgctgcag ggggaactgg gctgggacgc
    18181 taacgaataa atggatcgcg ttgctccagt ccagggtctg tcctgcgttc agtcaagacc
    18241 acggtcaaac cagtgccgca gggatgtggg ggcccccact gtcttgcatc caacaacaaa
    18301 agagcgccaa gcgctctctt gttgatcgga aggcaggttg gtgaattacc tgcactgtgc
    18361 cggggcgtac ttggccagca cggtgccggc agcgccgacc gtgatgccgg cagccgtggc
    18421 agaagcgttg gtggccgatg cgcaacccca gtcaatggcg ccagagttgc ctgcagcgat
    18481 cgcggcggcc agggactgac ccgcccctgc ggcggcgttg cgcacccagg gggtaagcgt
    18541 gatcctgttc gcagctgcgg cgacacctac actggtgggg ttgtaggtga cggtaatcag
    18601 gccgctggct gcggtgatca gcaccgagtt gacgaacttg gagttggcgc cggtgccggc
    18661 tgcctgtgcg ttccaggtgt tggcgacacg ggtcagatca gctgcggagg ccgagccctc
    18721 agccccaatc gttgccttgg ccggttccgc caggctcaga ccttcggtga tccgcgtacg
    18781 caccgtgtag tcctggtacg ccggcagcgc gatggcagcc aggataccga tgatggccac
    18841 cacgatcatc agttcgatca gggtgaagcc ttgttgcagt tttttcatgt cagtgtcctc
    18901 aaaaaagtag tggaacgaag ccttggagac gaagtgcgtc gccgcacgcc ctgaagggat
    18961 gcaggcttcg tgccaggggg ccgggcggcg gcggggcgtg acttgtttca cgctggcggc
    19021 ccgggcggcg gggggcgggt gacatttctt gtcgccccgc ggccggccgg ggctgacgaa
    19081 atgcggcagc ggcggcgcgg ccggcgccgg cagttgtcac tttttgtcgc tttggtgtcg
    19141 gcctgtcggg ccgatccggg gtggcggctg aaacgctatg ctatcgatag ctgctggcgc
    19201 agtctgggtg cgcgctgcat gcctttttga tggttgatcg gcaggtggcg ggccgggcgg
    19261 cgctggcctt gcagggcgcg caggcgcctt gggggccgcg gcgctcagcc ttgcccggca
    19321 cgccgatcac ggcacggatg ggcgcgccca acggccgctc ccatggatcc ggccccatga
    19381 agttccgacc gctcgccctg agcccgtcga agggcttcga caagctcagc ccgaacggaa
    19441 ttcgaggttt caaagggccg ggccaacagg ctcagtacag gcgcaccgcc tggccttggc
    19501 ggtcgaacgg gatcaggcct tcgtggcggc cggccttgat ggcgtcgacc atgcgctgca
    19561 gcggcgcttg ggcgtcggcg ctgctgggcg cgcggtgcag cgtgccgccc agcgcggcgg
    19621 cgaacaccag ggtccacgcg gggtgcagct gggtggcttg gtgcgccagc gcgtcgaagc
    19681 tgacgagttc gtccggcgtc ttgtcgacgc acaggcgcgg caccagggcg ccgccttcgc
    19741 cgcgcgcaaa gccggcgcgc tgctcgggcg tgggggcgtc gggcagctcg gcttcggtga
    19801 acacgaacag caggcgctgg ggctcgatct gggcgcgcgc ggcgcgcagc aggtcgtcga
    19861 aacaggcgat gtgcatgggt ctggctttcg atgcggggcc gccacccggc ccggcggcgg
    19921 ctggccgatt gtgaccccag tgccgcgttt tgcgcgcggt caggtgcttg tagcgagtcg
    19981 ccgaggggcc agcggcgcgg cgcgcggcgc gccaaatgtg acgggcgtgt aaagacggtc
    20041 ggcgccggcg gggcggcggg cgttgacgca gaggtaccgg cccacgggag cggccgggtg
    20101 ctggtttgag gatgcgtgcc atggatccgt ctgctgtcac cctttttcgc cttgccgtgc
    20161 tgtgcgcgct gggcacggcg gccacgaccg ccggcgcaca gtcgatggtg acggcgcgcg
    20221 tgctgtcggc gcagccggtg gtcgagcagg tgccggtgtc ggactgcggg ccctatggcg
    20281 cgccgccgtc cggcgccggg gcggcggtgg gggcgctgac gggcgggctg atcggcagcc
    20341 agctgggcaa gggcagcggc cacattgccg gcgcggtgct gggggcgctg ggcggggcgt
    20401 ttctgggcaa cgcggccgag gccacgcagt acggcggcta cggcggttgc gccacgcgct
    20461 acagccagcg cgtgaccggc tacgacgtcg tttatgaggt ggcgggccgc agctaccaga
    20521 cgcgcatggc gcaggcgccg ggccagtgga tccaggtgcc cgcgcccgac gcgtaccacg
    20581 gccagccggc ctggggcgcc ggcgaggtgc agagctaccc ggtggcgccg ccgtcgctgg
    20641 cgggctaccc gatgccggtc tacccggcgg cgggtgaatc gtcgggcgtg gtgacggcgc
    20701 cgccggcgtg gggcggccag ccctacccgg tgccgtaccc gggcggttac gggccggtgg
    20761 tgccgcaggg ttatgcggcg ccctacccgc cggcctatgc gccgccggcg tacccggtgt
    20821 acccggccta cccgcccgcg gcctacgcgc cgccgccggt gcgctatgtg gcgccggtgg
    20881 gggtcaacct gtcgatcggc ggcgtgatcg gcggccgcgg ccgcggtcat ggccactggg
    20941 gggtgggcat aggcagcggc tggtagggcg ttcggggccc cgcccctcgc cgcaggggcg
    21001 agggcgaccc ggcgtgcggg tgttcacccg ctttgccctg gccaagggcc ggcgcgaggg
    21061 ccggcggcga ccgcctggcc ttcaggcgcc cggctggtac atgctcagcg gcagcttctc
    21121 gcgcgccatg gcgcgcgcca cgccgggcac ctgggcggtg cgctgggcat gggcccacag
    21181 ggcgggggtg tcttcggggt tgatgtcgac catgctgccc cagcgcagca gcgtcagcgc
    21241 ataggcgtcc agcgggccgc agtgcacgcc gcccagccag tcgcggccgg cgctgtgcgc
    21301 ctgctgcacc agcgcctgca gctcgcccag ctgctggcgg tacgccgtga gcgcctgggc
    21361 gcggatggac tgctgcgccg ctgggtcgtc gctgaacttg tgcggcatga agatgtgggt
    21421 gaaggtgggg tgcacggtgt tgttcatcca cgccagcgct tcgacgaaac gcgcgcgcgc
    21481 cagcggctcc ttgggcacga aggcgtgttc gggaaagcgc gcgtcgatgt aggccacgat
    21541 ggccatgagc tgggtgatga cggcgtcgcc gtcgaccagc accggcacct ggccgcgcgc
    21601 gttcagcgcc aggtagtcgg ggttgcgctg ttcgttcttg tgcagccgga tggccatcgg
    21661 ctcgaaggtg gcgccggcga tttccagcat ggcgtgcggc acgaacgagc aggcgccggg
    21721 ggcgtaatag agcttgaggg tcatgggacg ggtctccggt ggttctgggg cagcgcgagc
    21781 atagcgcgcg gccggctcat cctggggttg cggcctttgg aggccctgaa caccgttcgg
    21841 gccgagcttg tccaaggcat cggttttgtc caagccatcg gttcattcgc aggggcgcca
    21901 gtcgtgcccg ccgagggcgc cgttgagggc gcgcgcctgc cggcgcagcg cggcgtcggc
    21961 ctgcggcagg cgcagcaggt aggccggtgt gtcgcgccgc tcgcgcacca cgcgggcgcc
    22021 gcgcacgccc ttgcgggcca ggttgtcgag cgcgcgctcg gccgcctctt cgctggcaaa
    22081 gcgccccagc gacaggccgg gctccagcgc cgcgccgggg cggtcggtgt ccacgccctg
    22141 ggcgcgcagc tcggcgcggc gcgcggccac ggcggcggcg tcgggcagct tgccgagata
    22201 caccatccag cggcccggca gctgcaccgc atcgacgcgc cagctgccct cgggcaggcc
    22261 ggcggcggcc tggcgcacgg cctcgatctg gctggcgtcg aaggtgccga tctgcaggca
    22321 ggcggtgggc gcgggggcgc tggcggatgc ggcggccggt tcggcggcgc tggccggggt
    22381 ggccgaggcg gcggtgctgc ggcgcgccgg ggtgtcgtcg gccgccgcgg cggccgaagc
    22441 cggctccagc ggctgcaggc gcagggcctg gggctgcacc tggcgctgca ggcgttgcgg
    22501 ctcggcctgc tcttgcggca ccagccccag cgggcgcagc cagccctggg tccaggccag
    22561 ccagcccagg ttggcggcca gcagcagcag caccagccgg ccgatcatgc gggcgcccca
    22621 ggcgctgccg ggcgcacgct gacttcggcg ctgcgcactt cgcgcacgcc ggtggcgcag
    22681 cgcacgcgca gcgcgccatc ggggccgacg cccagggcca cgccttcgct gccgtcggac
    22741 agctgcaccg gctggccggc cagcaggtcg cgggtggcaa agcgcggcgc cagcggcgca
    22801 aagccggtct gctcgaaggc gtgcagcgca tgcaccagcg gcaccgcgat gcgcagcagc
    22861 gctcccgggg cgtccacccc gggcagcagc gcctgcagcc aggccggcgg ggtggcgaag
    22921 ccggtgccgt cgagcggcgc gatgttgacc ccgatgccga cgatggcgca gcgcccggcg
    22981 ccggcggccg ctgcgccgct ggcggtttcg accaggatgc cggccagctt gcggccctgc
    23041 caccacaggt cgttgggcca tttgaggcgg atgtcggggt gcagcgcctc ggccaccgcc
    23101 aggcccacgg ccagcgacag gcccgaccag tcgtgcggcg ccagcggcag cgccagcgaa
    23161 aacgccagcg cgccgcccgc ttcgccgtcg cccgactgcc agccgcggcc cagccgcccg
    23221 cggccggcgg tctgcagctc ggccaccagc agggtgggcg gcaggcggcc ggcgcgggcg
    23281 cggcgcatca gctcgctgtt ggtggagtcg atttgccccg ccacttcgac caccaggccc
    23341 ggcagcagcg gcgtcagcgc gctgcgcagc gcgtcgaccg gccaggacag ggtgggggcg
    23401 gtcatggcgg cggctgccgg ccggacttgc ggcccgaccg gcggcggccg gcctggtctt
    23461 cggcgctgtc gaggtcggac gacagcaggg tgccgcggca cgccctgctg ccgcagcggc
    23521 aggggtagtc ggcccgcagt tcgtcggtca gcggctcgtc gatcaccagg ccgtagtcgt
    23581 agttgagctc ttcgccggcg cggatggcgc ggcgcgcggt gatgaagatg cgcccgccct
    23641 gctcgtcggc cttgcagttg gggctgcacg agtggttgat ccagcgcgag gcgttgccgc
    23701 cgtacagcgc gtcgatcacg tggccgtcgt cgatgtggaa gtagaaggtg tggttggggt
    23761 cctgcgggtc gtgcgggtgg cggcgctgcg cctcgtccca ggagatcacc tggcctacgt
    23821 attcgatgat gcgcgtgccc ttgggaatgt ccaccagcgc gaacacgccc ttgccgtgca
    23881 cgccgctgcg gcgcacctgg atgcggcgcg gcgccgcggt cttgcgaggg gtggggttgg
    23941 ccacggcggg aaatttgcta tgttgaatgg tgtgtgcgcg cgtgcatggg caggcgtgcg
    24001 cgggcgggtg cgcgtgggcg cacgcgcgcg agagcaggaa attctagcga catgaagaca
    24061 ctggtcatag ccgagaagcc gtcggtggcg caggacatcg tgcgggcgct cacgcccgtg
    24121 gcgggcaagt tcgacaagca cgacgagcat ttcgagaacg agcgctacgt ggtcaccagc
    24181 gcggttggtc acctggtgga gatccaggcg cccgagcaat tcgacgtcaa gcgtggcaag
    24241 tggagctttg cccacctgcc ggtgatcccg ccgcacttcg acctcaagcc ggtcgacaaa
    24301 accaggacac gcctgaacgc ggtggtcagg caggcgcggc gcaaggatgt gggcgagctc
    24361 atcaacgcct gcgacgccgg gcgcgagggc gagctgatct ttcgcctgat cgagcagtac
    24421 gccggcggcg ccaagccgct gggcaagccg gtccggcggc tgtggctgca aagcatgacg
    24481 ccgcaggcca tccgcgaagg cttcgagcgg ctgcgcagcg acgcccagat gcagggtctg
    24541 gccgacgccg cgcgcagccg cagcgaggcc gactggctgg tcggcatcaa cggcacgcgc
    24601 gccatgacgg ccttcaactc gcgcgacggc ggctttttcc tgaccaccgt ggggcgggtg
    24661 cagacgccca cgctggcggt ggtggtcgag cgcgaggagc agatccgccg ccatgtggcg
    24721 cgcgattact gggagctgca cgccagcttc gaggccgaag ccggcagcta cgtcggcaag
    24781 tggttcgatc cggactggaa gaaggacccc gaggacgccg aaaagcgcgc cgaccgcctg
    24841 tgggacgagg cgcgcgcgcg cgccattgcc gccgccgtgc agggccagcc ggccagcgtg
    24901 cgcgaggaaa gcaagcccag cagccagctc agcccgctgc tgttcgacct caccagcctg
    24961 cagcgcgagg ccaacggcaa gttcggcttt tcggccaaga ccacgctggc gctggcgcag
    25021 agcctgtacg aaaagcacaa ggcgctgacc tacccgcgca ccgactcgcg ccacctgccc
    25081 gaggactacg tgggggtggc gcgccagacc ttcgagatgc tggccggcgg ccaccagacg
    25141 cacctggcgc cgcacgccag caaggcgctg gccgaaggct atgtcaggcc cggcaagcgc
    25201 atcttcgaca acggcaaggt gtcggaccac tttgccatca tccccacgct gcaggagccc
    25261 ggcgcgctat ccgaagcaga gcgcaagctg tacgacctgg tggtgcgccg cttcatggcg
    25321 gtgttctatc cggcggcgca gtaccaggtc accacccgca tcaccacggt ggagcatggc
    25381 agcgaacgcc atcggtttca aagcaacggc aaggtgctgg ttcagcccgg ctggctggcc
    25441 atctacggca aggaagccga aggcgagcag ggcgaggaca aggacggcaa gtcgctggtg
    25501 ccggtgcgcc cgggcgagca ggtgatgcac cgggacaccg aggtcaaggc gctggccacg
    25561 cgcccgccgg cgcgctactc cgaagccacg ctgctgggcg ccatggaagg cgccggcaag
    25621 tggatcgacg acgacgagct gcgcgaagcc atgcaggaca agggcctggg cacgccggcc
    25681 acgcgcgcgg ccatcatcga agggctgatc ggcgaaaagt acctgctgcg cgaagggcgc
    25741 gagctgatcc ccaccgccaa ggcgttccag ctgatgacgc tgctgcgcgg cctgcaggtg
    25801 gaggagctga gcaagcccgc gctcaccggc gactgggaat acaagctggc gcagatggag
    25861 cgcggccagc tggcgcgcgc cgacttcatg cgcgaaatcg ccgccatgac cgagcgcatc
    25921 gtgcgcaagg ccaaggagta cgaccgcgac agcgtgccgg gcgactacgc ccagcttgcc
    25981 acgccctgcc cgcagtgcgg cggcgtggtg cgcgaaaact accgccgcta cgcctgcacc
    26041 ggggcggccg gcgccggcga aggctgcggt ttttccttca ccaagacgcc gggcgggcgc
    26101 acctttgcca ccgacgaggc cgagcagttc ctgcgccagc gccgcatcgg cccgctggaa
    26161 ggctttcgca gcaaggccgg ctggcccttc gtggccgaac tggcgctggt gcgcgacgag
    26221 gccgcgggcc agtggaagct ggagttcgac ttcggcgacg acgcccgcgc cgaagccagc
    26281 ggcgagatcg tcgatctgtc cgaccagccc agcctgggcg cctgtcccaa gtgcggcggc
    26341 gccgtcaagg cctacggcaa gagctacctg tgcgaacgca gcctgccgtc gcacgcgcag
    26401 cccacgccca gttgcgactt caagagcggg caggtgatcc tgcagcagcc ggtcgaaccc
    26461 gagcagatgc gcaagctgct ggccgagggc cgcaccgacg tgctcgaccg cttcgtcagc
    26521 ctgcgcacgc gccgcccgtt caaggcgcgc ctggcctgga acgccgacga aggcaaggtg
    26581 ggcttcgagt tcgagccgcg cgaaggcggc cgcaaatacc cgccacgccc ctcgaccgct
    26641 tccaaaacaa tagctgctgg cgcagcaccg gcgggcgctg tggccaggaa atccgccaag
    26701 aaagcgaccg ccgccggaac cgccgccaag acccgggctc ccaaggccga caaggccccc
    26761 aaggccccgc gcgccggcaa cctgcacccc agccccgaac tggccgccgt catcgggccg
    26821 ggaccgttcg gccgcggcga ggtgaggaag aagctgtggg actacatcaa ggcccacggc
    26881 ctgcaggatc cgcaggacaa gcgcagcatc gtcgccgacg acaagctcag gcccattctg
    26941 ggcgccgagc gcgtgggcat gttcaagctg gccggcctgg cgggcgcgca cctgaaggcc
    27001 tgagcggcgc tgcgcgccct tctcccgtac cgaccaccca tcacgcacca ccatgaacat
    27061 cacccaggac accgccgtca cgctcgacta ccgcatcacc gacgcccagg gcaagccgct
    27121 ggacgccggc accaccgcct acctgcacgg cggctacgac aacctgttcg ccaaggccga
    27181 agcggcgctg caaggcaaga ccgtgggcga caaggtggcg ctggcgctga gcgcggccga
    27241 agccttcggc gcgcgcgacg aggcgctggc gcgccgcatc ccgaaaagcg agttcccgcc
    27301 cggcgtcaag gtgggcggcc agttgcaggg cgccgggccg cacgggccgg tggtctaccg
    27361 cgtggtcaag atcaagggcc ccgaggtgct gctggacgcc aaccacccgc tggccggcct
    27421 ggacatccgc ttcgccgcca ccgtcaaggc ggtgcgcgcg gccaccgccg aggagatcgc
    27481 gcaccgccac gtgcacggtg cgcatgggca tcaccactga ccgcaaagct cctgaatgca
    27541 tagctgctgg cgcttgccag acaagcgctg caggccaatg aaacatatga ccaaggtggc
    27601 gtgccggagc gcgccggcgg gcggcgcagc gccggggccg gaggcctgat ggcgcctgct
    27661 tcggacccgc acgccgcggc gccgccgctt gccgcccgtc aagcctgggc catgctgctg
    27721 gcgctgaccg gcgccttcgt gctcagtcag gcgtttcgca ccctgggcgc catcatgggg
    27781 ccgccgctgg cggcgcagct gcagctcagt gcgcagcagc tggggctgtg gtcggcggcg
    27841 tttcactttt cgttcggcgc catgcaactg gccatgggcg tgtcgctgga cctgtggggc
    27901 gtgcgccgca ccatcctgct ggcgtttccg gtggccgtgg cgggggcgct gctgtcgtcg
    27961 caggccgacg gcttcggcat gctgctgctg gggcaggcgc tgatcggcgc cggctgcgcg
    28021 ccggcgtttc tggtgtgcac ggtgttcatc ggccggcatt tcgcgcccga gcgctttacc
    28081 gccatgtcgg gcctgaccat gagcctgagc ggcctgggcg tgctggccac cgccagcccg
    28141 ctggcctggc tgatcgagcg cagccactgg cgctggggct tcggcgtgct ggccgggctg
    28201 tcggtgctgg tgtggctggt gatcgcctgg cgcgtgcacg agccggcccg cccggccgac
    28261 gccggcgcgc cgcgccccac cgcgctggac gcgctgcgcg gcctgctggg cctgtttgcg
    28321 ctgccgcaga cggccggcgt ggtggcctac gcggcggtcg gctacgccgc cttcatcacc
    28381 ctgcgcgggc tgtggctggg gccgctgctg gtgcagcgct tcgacctgtc gctgctgcag
    28441 gccggccacg tggcgctggc catgacgctg gccagcatgg ccagcccggc catcttcggc
    28501 cggctcgacc cgggcggcgc gcgccggctg cgctggctgc tgacgctggc gacggtggcg
    28561 gcgctgctgc tggccaccat cggtctggtg ccgctgctgt ggctggacgt gctgctggcc
    28621 ggcagctacg ggctgatctc gggctttggc gtgctgcagt acggctacgt caacgacgcc
    28681 tacccgccgg cgctgcgcgg gcgcgccatg tcgctgctga ccatggccat gttcctgggc
    28741 gtgtcgctga tgcaatgggc cagcgggctg gccgccagcc tggcaccgac gctgggcgtg
    28801 gagccgttca gcgccgccct gctgacgatg gcggccatgc tgctggccgg cgcgctggcc
    28861 ctgtggaagc tgccgcgcgc gccactggcg cagtgagcgc gaggccgcgc ccag
//
LOCUS       DAOQTZ010000021        28352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_1962_length_28352_cov_12.836626, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000021 DAOQTZ010000000
VERSION     DAOQTZ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 28352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28352
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1962_length_28352_cov_12.836626"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKN51_04145"
     CDS             complement(<1..328)
                     /locus_tag="PKN51_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNT84322.1"
                     /translation="MKRMLINATQAEERRLAIVDGQKLLDYEIEIEGREQRKGNIYKA
                     VVTRVEPSLEACFVDYGEDRHGFLPFKEISKQYFAPGVSPSQARINEVIKEGQELLVQ
                     VEKEERG"
     gene            963..1934
                     /locus_tag="PKN51_04150"
     CDS             963..1934
                     /locus_tag="PKN51_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT84323.1"
                     /translation="MPAAKPAVLRLTVDADSGGQRLDNYLLRHLKGVPKTHVYRIIRS
                     GEVRLNKGRAAADTRVSEGDEVRVPPVRVAAPVQHLDGRAAPAREFPVLLEDEQLIAI
                     DKPAGVAVHGGSGVSFGVIEQLRQARGYGQPGGLRFLELVHRLDRETSGILLLAKKRS
                     ALKHLQNQFRERQTGKTYLALVLGEWPAARRVIDAPLHKYLLADGERRVRVVAPDDPD
                     GMRAITLVKVRQRLAAFTLLEVTIKTGRTHQIRVHLAAQGHPIAGDDKYGDFERNKAL
                     QKQGLRRMFLHAWRLQFSHPERAERIELQAALPPDLLSFTKHLAPAP"
     gene            1931..2593
                     /locus_tag="PKN51_04155"
     CDS             1931..2593
                     /locus_tag="PKN51_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNT84324.1"
                     /translation="MSRPRQFDLICFDWDGTLFDSTAIIVRSIQQAVLDVGGARPSDA
                     AAAYVIGMALVPALARAAPDVPADKYPELAARYRHHYLARQHDISLFEGVLPMLAELR
                     ARHHWLSVATGKSRRGLDEVLASRQLQGVFDGSRTADETAGKPHPRMLHELMREFGVE
                     PQRTLMIGDTTHDLQMALNAGCASVGVSYGAHAPDDFEALRPRQVAHSVAELHRWLSD
                     NA"
     gene            2638..3015
                     /locus_tag="PKN51_04160"
     CDS             2638..3015
                     /locus_tag="PKN51_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNT84325.1"
                     /translation="MDNDLSPVYLCDSADLQEGGLAVPFDVAHGGENLRAFAVRFEGR
                     VHAYLNRCAHVPMEMDWQPNRFFDDSGQWLLCATHGATYAPDTGHCVAGPCSGAQLVR
                     IELIEAAGQVHWQPTGALRPIAF"
     gene            3074..4030
                     /locus_tag="PKN51_04165"
     CDS             3074..4030
                     /locus_tag="PKN51_04165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNT84326.1"
                     /translation="MENEVYVNGQPVPWERATIEKLLFAELRERRAARRWRTLRTVLW
                     LGLIGALAWLLWSDHLPSATPSSAHTAMVSIKGEISDTGEGSAEYILPALRSAMEDKG
                     AQALVLLINSPGGSPVQAGLIHDEVRRLRALHDKPVYAVVEESCASAAYYIAAAADRI
                     YVDKASLVGSIGVLIDGFGAVGALEKLGVERRLLTAGENKGFLDPFSPQSEAHRAHAQ
                     RMLDQIHQQFIDVVRQGRGERLKETPDMFSGLFWTGEQAVQLGLADQLGSLDMVAREV
                     VKAEKLVDYTNKQNFAERFAKRLGAQVGEGALAALRGAPGWR"
     gene            complement(4109..6364)
                     /locus_tag="PKN51_04170"
     CDS             complement(4109..6364)
                     /locus_tag="PKN51_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT84327.1"
                     /translation="MSKQQAHPSPSSAANGVPALFDRAVRLFNGGQPDQAAPLCQQVL
                     QQQPNHAHAWQLLGMVHVVGNRPAEALPALQRALALNPRQTQILCLLGTVLARLGQSE
                     QAIDHFRQALALAPQDANIWYDLGNVQHGLGQHLQALASHDRALALAPGHADAWLNRG
                     HALLELHRLGDAIASFERAIDLRPRASTWLLLGSALEQAQRLPDALACFEKVVALEPD
                     NLDGWLKFGGVLLQLGRPDLALERIEVAQRLAPQSPRVLLTQAAALAKTRGDAAALMP
                     LLQAHALAPDDLSVSMQLLYFMLKTAHWEGLTEVLAQTLRHWRAGARDANTLTLLAHP
                     DVSAADLLQIAQAYHAPLNDAFRAMHAKVARGAPAGRRLRLGYLSSDLRHHAVGFLMT
                     GVFEAHDRQRFETFAFSIYPQPDRSPERQRAQAAFDHFIDIHHLGDDEAAALIAGHGI
                     DLLVDLNGLTTFSRPGILARRPAPVQVQYLGFPGTSGLAAIDYILGDRWVTPVEQAQA
                     FSEHIVRMPDCFQANDDQRRFAAATPTRRELGLPDQGFVFCCLNNSYKILPGVFDGWM
                     RLLQNTPGSVLWLLGDNDTARSNLRAHAQARGVAPERLVFAERRPYDQYLARYRQADL
                     FLDTLPFNGGTTVSDALWAGLPVLTQVGDRFAGRMAASLLDCIGLPELITHSQPEYEA
                     LAHRLATEPGLLQGLRDRLAANIRTRPLFDTRRFTRHLERAFEHMIERHAQGLPPQGF
                     DVAELPLAQSA"
     gene            complement(6423..7208)
                     /locus_tag="PKN51_04175"
     CDS             complement(6423..7208)
                     /locus_tag="PKN51_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNT84328.1"
                     /translation="MSAAPTPGTLYLVPTPLDHGCPLQAPLTDALPQGTIEVAARLSH
                     WISENARSTRAFLKRVAALVPLAQPLQAQHIQQLPHAIHKQGDHRRAGTWDARPLLAS
                     ALQGHDLGLVSEAGMPAVADPGSSVVRAAHDLGIEVRPLVGPVSMLLALAASGLNGQS
                     FAFVGYLPQEASRRATRLRELEAWAQQSGQSQMFIETPYRNAALLQALLQQLRPDTRL
                     AVCSGLTLAQQRCTSLRVADWRLRSAALTADLSAQPAVFAIGP"
     gene            complement(7205..7813)
                     /locus_tag="PKN51_04180"
     CDS             complement(7205..7813)
                     /locus_tag="PKN51_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNT84329.1"
                     /translation="MPTDTTDASAAPPLVLGSTSPYRRELLSRLRLPFTTAAPQVDET
                     PLPHETPAALARRLALAKAREVAARHPQAIVIGADQVADLAGEPLGKPGSHERAVAQL
                     QRMSGQTVVFQTALSVVCLASGFEQSELAPVEVRFRALDAGEIERYLRAEQPYDCAGS
                     AKSEGLGIALLEAIHNDDPTALVGLPLIRTARLLRAAGLRLP"
     gene            7840..8391
                     /locus_tag="PKN51_04185"
     CDS             7840..8391
                     /locus_tag="PKN51_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNT84330.1"
                     /translation="MSRSFDARRLDVAALAEAGASLSGADELQNYERLALEAQGADKD
                     LTVEWKVQGGRRQAVDGQWRPSLHLQAQTRLPLTCQRCLGPVATPLVVDRHLVFVESE
                     ARAAALDDASDDDVLALTPALDLRELIEDELLLALPLVPRHEVCPDAVRLSAHSEGFD
                     AAPPQRPHPFAALAALKQGGKGR"
     gene            8549..8731
                     /gene="rpmF"
                     /locus_tag="PKN51_04190"
     CDS             8549..8731
                     /gene="rpmF"
                     /locus_tag="PKN51_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNT84331.1"
                     /translation="MAVQQNKKSPSKRGMHRSHNALSVPGIAVEPTTGETHLRHHISP
                     NGFYRGRQVIKSKTEA"
     gene            8917..9954
                     /gene="plsX"
                     /locus_tag="PKN51_04195"
     CDS             8917..9954
                     /gene="plsX"
                     /locus_tag="PKN51_04195"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNT84332.1"
                     /translation="MIVLAVDCMGGDHGPAVTLPACRQFLDRHPEAELLLVGQPEALA
                     GLTHERARVIAASEVVTMDDAVEVALRRKKDSSMRVAIQQVKDGAAQAAVSAGNTGAL
                     MAVARYLLKTLEGIDRPAIATQIPNQKGGATTVLDLGANVDCTAEHLLQFAVMGSALV
                     SVLNDDPQPTVGLLNIGEEAIKGSEEIKRAGELLRAAGDAGDLNFVGNVEGNDIFKGT
                     ADIVVCDGFVGNVALKTIEGLASMIGGFMKEEFSRNLLTKAAAVVAYPVLAALKRRMD
                     HRRYNGAALLGLRGLVFKSHGSADALAFEQALVRAYDAASHQLLARVQQRIAHAAPLL
                     AASGAAAAPAA"
     gene            9996..10976
                     /locus_tag="PKN51_04200"
     CDS             9996..10976
                     /locus_tag="PKN51_04200"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNT84333.1"
                     /translation="MSRRYSRITGTGSYLPPRRVTNADLVAELASRGIETSDEWIVER
                     TGIRARHLAAPDVPASELAVPAARQALAAAGLQPGDIDLIIVATSTPDMVFPSTAAIV
                     QHKLGMAGCPVFDVQAVCSGFVYALTVADALIANGTARRALVIGAEVFSRILDWSDRT
                     TCVLFGDGAGAVVLEASDRPGILATDLHADGKYVDILCVPGGVSGGQVLGDPLLKMDG
                     QAVFKLAVGVLDKAARAVLAKAGKSEADIDWLIPHQANLRIMHGTARKLHLPLDKVIV
                     TVNEHGNTSAASIPLALDHGVRSGQIQPGQTLLLEGVGGGFTWGAVLLNM"
     gene            11037..11987
                     /gene="fabD"
                     /locus_tag="PKN51_04205"
     CDS             11037..11987
                     /gene="fabD"
                     /locus_tag="PKN51_04205"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNT84334.1"
                     /translation="MKPFAFVFPGQGSQAVGMLDAWGDHPAVRDTLADASAALGEDLG
                     ALIAQGPKEALALTTNTQPVMLVAGVACWRAWRAEGGVAPAAVAGHSLGEYSALVAAE
                     VLTLAQAVPLVRLRAAAMQQAVPVGTGAMAAILGLDAQKVIAGCAEATAAMGQAGAEV
                     VEAVNFNDPAQTVIAGSKAAVDKACEVLKGLGAKRALPLPVSAPFHSSLMKPAAEQLK
                     AALAALELGAPRIPVVNNIDVAVEQEADRIRDALYRQAFGPVRWVECVQKIRALGIDT
                     VVECGPGKVLAGMTKRIDAGLTGAALYDPATLAEVKALLG"
     gene            12016..12765
                     /gene="fabG"
                     /locus_tag="PKN51_04210"
     CDS             12016..12765
                     /gene="fabG"
                     /locus_tag="PKN51_04210"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNT84335.1"
                     /translation="MSDTPFAGQVALVTGASRGIGAAIAAQLAERGLKVIGTATTEDG
                     AARIGAALASHPGCRGAALDVNDAAAAEALIEAIVKEHGALHVLVNNAGITRDMLAMR
                     LKDEDWDAVLDTNLKAVFRMSRAVMRPMMKQRYGRIVNITSVVGASGNPGQANYAAAK
                     AGVAGMTRALARELGSRGITVNCVAPGFIATDMTAALGEEQHKALAAQIPLGRLGQPA
                     DIAAAVAYLASPEAGYVTGQELHVNGGMFMA"
     gene            12868..13104
                     /gene="acpP"
                     /locus_tag="PKN51_04215"
     CDS             12868..13104
                     /gene="acpP"
                     /locus_tag="PKN51_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNT84336.1"
                     /translation="MSDIEARVKKIIAEQLGVEESQVTNEKAFVADLGADSLDTVELV
                     MALEDEFGIEIPDEDAEKITTVQAAIDYAVSKKA"
     gene            13268..14512
                     /gene="fabF"
                     /locus_tag="PKN51_04220"
     CDS             13268..14512
                     /gene="fabF"
                     /locus_tag="PKN51_04220"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNT84337.1"
                     /translation="MNRRRVVVTGLGCVSPVGNTVAESWASLLAGRSGIDLITRFDAS
                     AFASRIAGEVRGLDISPHISAKEARTMDTFIHYGIVAAAEAVQDAGLATGEALDDEAA
                     TRIGCVVGSGIGGLPLIEETQTEYAARGPRRISPFFVPASIVNMVAGHVSMKFGFKGP
                     NLAIATACTTGLHCIGEAGRLIEYGDADVMIAGGAESTVSPLGVGGFAAMRALSTRND
                     DPKTASRPWDKDRDGFVLGEGAGILVLEAYEHAKARGARIYAELAGFGMSADAGHMTA
                     PNMDGPRRAMVAALRNAGLNADQVQYVNAHGTSTPLGDINESNAIKAALGDAARRAVV
                     SSTKSMTGHLLGGAGGIESVFSVLALHHQKVPPTINLFNPDPECDLDYCANSARDMKI
                     DAVLKNNFGFGGTNGSLVFRRL"
     gene            14598..15056
                     /locus_tag="PKN51_04225"
     CDS             14598..15056
                     /locus_tag="PKN51_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84338.1"
                     /translation="MSYPVGRSAALARVLLGLAAAGWLALGLTYFLAPALIQLAPAAM
                     LSIAINALVTLGLWRFWRAQEARQLRWTGEHWLLHTPGTEPDRGGEGTGVEVRLDAQR
                     WLLLRFRDPAEAGAHWLWAQASHDPARWHLLRCALYSAAPSPAATQASRP"
     gene            15103..15723
                     /gene="rpoE"
                     /locus_tag="PKN51_04230"
     CDS             15103..15723
                     /gene="rpoE"
                     /locus_tag="PKN51_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNT84339.1"
                     /translation="MAPPPAPADSDALLVERAAAGDTRAYELLVIKYQRRIERLIGRL
                     VRDVDLVPDIAQETFLRAWRALPQFRGEAQFYTWLYRIAVNTAKKSLLELKRDPVLTE
                     SALHTSDDDDETSRRENELISESTPESELAAREIAAAVNAAVEALPEDLRQAITLREI
                     EGLSYEEIAHTMNCPIGTVRSRIFRAREAISARVKPLLSHQTGKRW"
     gene            15752..16402
                     /locus_tag="PKN51_04235"
     CDS             15752..16402
                     /locus_tag="PKN51_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HNT84340.1"
                     /translation="MNARIDQTLDLEAISALMDGQLRDDDAARALGMMTASGQARESW
                     QLYHLVGDVLRSADLAACGQDRAFAARLAATLAAQRPAEVAAPRAPALSPHALTRPAA
                     NDGLFGWRMVAGLASFAAVAVIGWGMLGDLGPQRQGGAQLAQGAAAPRTALQTVAGPS
                     PALSTAEPDEIRVADGPAVMLRDPRLDELLQAHRQVSATPALGGFVLNATYEGAGR"
     gene            16471..17457
                     /locus_tag="PKN51_04240"
     CDS             16471..17457
                     /locus_tag="PKN51_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HNT84341.1"
                     /translation="MVSLAGAQSIGQFGGGAPAGVVGPSQRSVAEWLARLQQGSRVPS
                     YIGTFVVSSASGAMSSSRIWHVCEGDVQLERVDALTGVPRSTLRRNDTVMTFLPDARV
                     VTLDQRQLGAVFPNLLNLAQGLPVADYYDARELGRGRVAGCDAEIVQLLPRDEWRYGY
                     RIWSERRTGLVLKTQTLNAHGRVLEQAAFSEVQLDAPVQAEQLRQMMANTQGYRIVKA
                     EHARTTPQAEGWQLTTMVPGFEARNCYRRPPSASASLVQCLFSDGLATVSLFMQPLDP
                     ARHQQEGAVAMGATQTLTRRVVDRSGQWWVTAVGEVPPATLQAFVRGLERIP"
     gene            17517..18992
                     /locus_tag="PKN51_04245"
     CDS             17517..18992
                     /locus_tag="PKN51_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNT84342.1"
                     /translation="MNKLSLAALAVPMLLSATLNLPLAAQAQAPAAPIVRGLPDFTDL
                     VEQVGPAVVNIRTVERVRTGIGRNGPMMDEEMQEFFRRFFGLPLPGVPRQGPRQQEPQ
                     EPAEREVPRGVGSGFILTADGFVMTNAHVVDGADEVLVTLTDKREFKARIVGADKRTD
                     VAVVKIEASGLPAVRIGDVSRLKVGEWVMAIGSPFGLDNTVTAGIVSAKQRDTGDYLP
                     FIQTDVAVNPGNSGGPLINMRGEVVGINSQIYSRSGGFMGISFAIPIDEAMRVSEQLR
                     ASGRVTRGRIGVQIDQVSKEVAESIGLGKPQGALVRGVESGSPADKAGVEAGDIITRF
                     DGRVIDKASDLPRLVGNTKPGTRSALTVFRRGQTRELNITIAELEPEQTARRPSQRGS
                     APPKASEATQVLGLTVTELTEAQKKELKVRHGVRVEAATDAGARAGLREGDVILSVGN
                     AEVRSVRDFEAAVAKAERGKPISMLVQRGELVQFVLVRPGR"
     gene            19469..21277
                     /gene="lepA"
                     /locus_tag="PKN51_04250"
     CDS             19469..21277
                     /gene="lepA"
                     /locus_tag="PKN51_04250"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNT84343.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRLISRCGGLSDREMSEQVLDSM
                     DIEKERGITIKAQTAALQYQARDGRVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALELGVEVLPVLNKMDLPQADPDNAKAEIEDVIGIDASEAIA
                     ISAKTGMGIDDVLEQIVAKVPPPRGQAQAPLRAMIIDSWFDAYVGVVMLVRVVDGRLA
                     KGERFRMMASGSAYEANQLGVFTPAQQAREVLEAGEVGYIIAGIKELKAAKVGDTITL
                     EKKLPGNLGPADRPLPGFKEIQPQVFAGLYPTEANQYDQLRDALEKLQLNDASLHFEP
                     EVSQALGFGFRCGFLGLLHMEIVQERLEREFDQDLITTAPSVVYEVEKADGEVIQVEN
                     PSKMPDQGKIHEIREPIVTVHLYMPQDYVGPVMTLANQKRGVQINMQYHGRQVMLTYE
                     LPLGEIVLDFFDKLKSVSRGYASMDYEFKEYRPSDVVKVDILLNGEKVDALSIIVHRS
                     QAQFRGRAVAAKMREIISRQQFDVAIQAAIGANIIARENIKALRKNVLAKCYGGDITR
                     KRKLLEKQKAGKKRMKQIGSVEVPQEAFLAILQVED"
     gene            21277..22245
                     /gene="lepB"
                     /locus_tag="PKN51_04255"
     CDS             21277..22245
                     /gene="lepB"
                     /locus_tag="PKN51_04255"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNT84344.1"
                     /translation="MPLLTAVILAAFVAYAAAWYVGILQGNFALLLLLATLVTGAYWL
                     GERFVFLPRRRAAAQALEAQAAQRRSELAAQGITQVDEGELTQARERVLAQPWWLDWT
                     AGLFPVIAVVFLLRSFVFEPFKIPSGSMVPTLLVGDLILVNKYTYGLKLPVIHTRLTQ
                     GQPVQRGDVVVFRYPPQPSVDYIKRVVGLPGDEVAYLNKQLTINGQPVPQTAVSDFFD
                     EQAMEYFKQFDERLGVTNHRIIVDGRRPAFVGGASDFEYKDNCRYSVEGVRCKVPPGH
                     YFMMGDNRDNSLDSRFWGFVPDGHIVGKAFFVWMNFGNLKRIGSFH"
     gene            22271..22639
                     /locus_tag="PKN51_04260"
     CDS             22271..22639
                     /locus_tag="PKN51_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNT84345.1"
                     /translation="MKHQGPSSQRGISFFGLLFLGVVLALLAIVGARVVPTASEFMTI
                     QKAVKKAAADGDTVPAVRASFDRTASVDYITSISGKDLEVTKQGDKVVVNFAYDKEIP
                     LFGPAYLLIKYRGSSAGGYD"
     gene            22698..23357
                     /gene="rnc"
                     /locus_tag="PKN51_04265"
     CDS             22698..23357
                     /gene="rnc"
                     /locus_tag="PKN51_04265"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNT84346.1"
                     /translation="MQERLQYHFRDESLLERALTHRSFSADHNERLEFLGDSVLGLGV
                     AHMLYQALGGGAEGDLSRLRAQLVRQDSLHQLALHLGLPPLLRLGEGELRSGGQRRPS
                     ILADALEALIGAVYLDGGHAPADALVRRLFEQVEISPAMSAAAKDAKTALQEWLQARK
                     MRLPQYEVVRVLGAAHRQTFEVRCQVAELNLEVLGQGASRRAAEQCAAASMLEQLQNR
                     P"
     gene            23354..24355
                     /gene="era"
                     /locus_tag="PKN51_04270"
     CDS             23354..24355
                     /gene="era"
                     /locus_tag="PKN51_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNT84347.1"
                     /translation="MNTPEIIADGADPERAGAQSGTPSAPDSGASVQRCGLIAIVGKP
                     NVGKSTLLNALVGQKISITSRKAQTTRHRITGVRTLTDGQGGGTQYVFVDTPGFQTRH
                     STALNKSLNKTVLGAIDGVDLVLFVVEAGSFTLQDAKVLSLLKPGTPALLVANKLDSV
                     HRRAEIAPWLKGMQERHPFAEFVPMSAKNPRDVARLLEICARYLPEQPWWYGEDELTD
                     RSEKFLASETVREKLFRLTGDELPYTSTVVIEQFKEEKSRAHGRLLRIAATIVVEREG
                     HKAMVIGQQGERLKRIGTEARQELEKLLDAKVFLEIWVKVRSGWADDEARVRSFGYE"
     gene            24442..25209
                     /gene="recO"
                     /locus_tag="PKN51_04275"
     CDS             24442..25209
                     /gene="recO"
                     /locus_tag="PKN51_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNT84348.1"
                     /translation="MKRVVHEPGYVLHHYDWSESSLIVEAFTRAHGRVALVAKGAKRP
                     SSNFRPVLLPLQPLLLSWGGDGEIRTLKVAEWGGGQVMPRGDALLSGFYANELLLRLL
                     AREDAHPRLFDAYAALVHALAAGDAEPLAAAALRAFELLLLRETGHLPALDAQTLTLA
                     PLQASEPYRLVAEGGLRAAHADDAAWLPGQSWRRLAASLADEAPWTATLTVVVGLPGE
                     QRGSLRRQLGELLHYHGGGATLRTRQLLQDAQRLMAT"
     gene            25233..25985
                     /locus_tag="PKN51_04280"
     CDS             25233..25985
                     /locus_tag="PKN51_04280"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNT84349.1"
                     /translation="MPTQLSVNVNKVALVRNTRHLGIPSVTRAAQLCLQAGAHGITVH
                     PRPDARHIRADDVHALAALMRGWPGREFNIEGNPTQNLMDFIRAVRPAQATFVPDSED
                     QFTSDHGWDLADAGVAARLRPLIDECQGLGVRVSLFMDPLPELMAAARAVGAERVELY
                     TEPYAAAWGTPEQGRQLQRYAVAAQAAGDAGLGVNAGHDLNLHNLGTFVARVPGLLEV
                     SIGHAFIADALERGYAATTEAYLSCIAQAGRS"
     gene            26044..26451
                     /gene="acpS"
                     /locus_tag="PKN51_04285"
     CDS             26044..26451
                     /gene="acpS"
                     /locus_tag="PKN51_04285"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNT84350.1"
                     /translation="MIHGIGTDICDVRRIRASVERHGERFAQKILSEAELAAWRRRSA
                     RWPERGLRYLATRFSAKEAFSKAVGLGMRMPMHWRRCEIANQPSGQPCIVLHGPLKDW
                     FEARHLVAHISVSDESDYAASFCVVERLPPVRE"
     gene            26528..27583
                     /gene="nagZ"
                     /locus_tag="PKN51_04290"
     CDS             26528..27583
                     /gene="nagZ"
                     /locus_tag="PKN51_04290"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNT84351.1"
                     /translation="MQHAPLIIDIAGTALTEDDGRRLAHPLVGGLILFARNWQDREQL
                     GSLCAEVRRLRPELLICVDHEGGRVQRFRSGGFTHLPPMRALGELWMQDAMRAQDAAS
                     ACGYVLGAELRACGVDLSFTPVLDLDWGPSGVIGDRAFHADARVVTMLAKSLAHGLRR
                     AGMACCGKHFPGHGFVAADSHTALPRDTRTLKAILAADAAPYGWLSSVLDAVMAAHVV
                     YPRVDARPAGYSARWLQAVLRGRLGFGGAIFTDDLSMQAARHLEGRTVTPVEAVLAAL
                     DAGCDLALLCNQSLGGGAVLDEVIDGLARAQVQGRWQPREASAARCQALLPREPAPDW
                     DALMRSPAYLHALALVP"
BASE COUNT         4451 a         9686 c         9871 g         4344 t
ORIGIN      
        1 tgccgcgctc ttccttctcg acctggacca gcagctcctg gccttccttg atgacctcat
       61 tgatgcgcgc ctggctgggc gagacgccgg gagcgaagta ctgcttggag atttccttga
      121 acggcaggaa gccgtggcgg tcctcgccgt agtcgacgaa gcaggcttcc agcgacggct
      181 cgacgcgcgt gacgacggcc ttgtagatgt tgcccttgcg ctgctcgcgc ccttcgatct
      241 cgatttcgta gtcgagcagt ttctgtccgt cgacgatggc caggcggcgc tcttcggcct
      301 gcgtggcgtt gatcagcatc cgtttcatga tgcgcgttcc ttcatgcaat aacagtccct
      361 ggttcctggt gggaacccac gatgcctggg acggcgcact gaaacggacg gaaccggccg
      421 gcgggcgact gagggcggga tgcagcaggc gcgtggtgcg ccggctgtgg ccgctgaacg
      481 ggggttcagc ggccgcggtc aggcggcggc gcgtggatgg gggcgagcgg tgccgggcgc
      541 aaaattgcta cataaaaagt agctgccggc gcttgcgggg tgcggtttgg cggcgaaaaa
      601 ggctcaaaaa gcgccagccg cagccagcgc ccgaaagcca tgatccagac catcagcacc
      661 acgtgtgtct cgcttcgatc cgatccgcag gcgggccagg gggcgacctg cgcatcttgg
      721 gtattccgtg ttgggtgttt cagtgcgtcg accggtctgc gctgttctgg cgtggctgcc
      781 gactcttgtg gggcagggcg ctttctggtg caggcacggc atcgaccttt tcactggcag
      841 gccgcggaaa tgggccggtg gaatagactt cccgttttcg ggatgcagct tccagttcag
      901 attcaaccac ttacggcggg ttgccagtga atcgcattat aggtgcccag gccgccccca
      961 cgatgcccgc ggccaaaccc gcagtgctgc ggctgacggt ggacgccgac agcggcggcc
     1021 agcggcttga caactacctg ctgcgccacc tcaagggcgt gcccaagacc catgtctacc
     1081 gcatcatccg ctccggcgag gtgcgcctga acaaggggcg cgccgccgcc gacacccggg
     1141 tgagcgaggg cgacgaggtg cgcgtgccgc cggtgcgggt ggcggcgccc gtccaacacc
     1201 tggacggccg ggcggcgccg gcgcgcgaat tccccgtcct gctggaagac gagcagttga
     1261 tcgccatcga caaaccggcc ggcgtggccg tgcatggcgg ctcgggcgtg agcttcggcg
     1321 tgatcgagca gctgcgccag gcgcgcggct acgggcagcc gggcgggctg cgctttctgg
     1381 aactggtgca ccggctggac cgcgaaacca gcggcatcct gctgctggcc aagaagcgca
     1441 gcgcgctcaa gcacctgcag aaccagttcc gtgagcgcca gaccggcaag acctacctcg
     1501 ccctggtgct cggcgagtgg ccggcggcgc gccgggtgat cgacgcgccg ttgcacaaat
     1561 acctgctggc cgacggcgag cggcgcgtgc gcgtggtggc gccggacgac cccgacggca
     1621 tgcgcgccat caccctggtg aaggtgcgcc agcggctggc ggcgttcacc ctgctcgaag
     1681 tcaccatcaa gaccggccgc acgcaccaga tccgcgtgca cctggcggcc cagggccacc
     1741 cgattgccgg cgacgacaaa tacggcgatt tcgagcgcaa caaggccttg cagaaacagg
     1801 ggctgagacg catgttcctg cacgcctggc gcctgcagtt cagccacccc gagcgcgctg
     1861 agcgcatcga actgcaggcc gcgctgccgc ccgatctgct ctcattcacc aagcaccttg
     1921 cgcccgcccc atgagccgtc cacgccagtt tgacctgatc tgcttcgact gggacggcac
     1981 cctgttcgac tccaccgcca tcatcgtgcg cagcatccag caggcggtgc tggacgtggg
     2041 tggcgcgcgc ccgagcgacg ccgccgccgc ctatgtgatc ggcatggcgc tggtgccggc
     2101 cctggccagg gccgcgcccg acgtaccggc cgacaagtac cccgagctgg cggcgcgcta
     2161 ccgtcaccac tacctggcgc gccagcacga catcagcctg ttcgagggcg tgctgcccat
     2221 gctggccgag ctgcgcgcgc gccaccactg gctgagcgtg gccaccggca agagccggcg
     2281 cggcctggac gaggtgctgg cctcgcgcca gctgcagggc gtgttcgatg gttcgcgcac
     2341 cgccgacgaa accgccggca agccgcaccc gcgcatgctg cacgagctga tgcgcgagtt
     2401 cggtgtcgag ccgcagcgca ccctgatgat cggcgacacc acccacgatc tgcagatggc
     2461 gctgaacgcc ggctgcgcca gcgtcggcgt gagctacggg gcccacgcac ccgacgactt
     2521 cgaggccctg cgcccgcgcc aggtcgccca ctcggtggcc gagttgcacc ggtggctgag
     2581 cgacaatgcc tgatccgtca cctcgaaaac tattgatttg atagctgctg gcgcaatatg
     2641 gacaacgatc tgagcccggt ttacctgtgc gacagcgccg acctgcagga aggcgggctg
     2701 gcggtgccgt tcgacgtcgc gcatggtggc gaaaacctgc gcgcctttgc cgtgcgcttc
     2761 gaaggccggg tgcatgccta cctcaaccgc tgcgcccatg tgccgatgga aatggactgg
     2821 cagcccaacc gctttttcga cgacagcggc caatggctgc tgtgcgccac ccatggcgcc
     2881 acctacgccc cggacaccgg ccattgcgtg gccggcccct gcagcggcgc gcagctggtt
     2941 cgcatcgagc tgatcgaagc ggccggccag gtgcactggc agcccaccgg ggcgctgcgg
     3001 ccgatcgcct tctgaagccc aaggccgagc ctcaaatcaa gttcaaaccc ttgcgcagca
     3061 agcgccacaa gctatggaaa acgaagtgta tgtgaacggc cagccggtgc cctgggagcg
     3121 cgccacgatc gagaaactgc tgttcgccga gctgcgcgag cgccgcgccg cgcgccgctg
     3181 gcgcaccttg cgcaccgtgc tgtggctggg cctgatcggc gccctggcct ggctgctgtg
     3241 gtccgaccac ctgcccagcg ccacgcccag cagcgcccac accgccatgg tgtcgatcaa
     3301 gggcgagatc agcgacaccg gcgagggcag cgccgagtac atcctgcccg cgctgcgcag
     3361 cgccatggaa gacaagggcg cgcaggcgct ggtgctgctg atcaactcgc ccggcggcag
     3421 cccggtgcag gcgggcctga tccacgacga ggtgcgccgc ctgcgcgcgc tgcacgacaa
     3481 gccggtgtac gccgtggtgg aagaaagctg cgcctcggcg gcctactaca tcgccgccgc
     3541 cgccgaccgc atctacgtcg acaaggccag cctggtgggc agcatcggtg tgctgatcga
     3601 cggcttcggt gccgtcgggg cgctggaaaa gctgggcgtc gagcgccggc tgctgacggc
     3661 gggcgagaac aagggctttc tcgatccctt cagcccgcag tccgaagccc accgcgccca
     3721 tgcccagcgc atgctggacc agatccacca gcaattcatc gacgtggtgc gccagggccg
     3781 tggcgagcgc ctgaaggaaa ccccggatat gttcagcggc ctgttctgga ccggcgagca
     3841 ggcggtgcag ctcggcctgg ccgaccaact cggcagcctg gacatggtgg cgcgcgaggt
     3901 ggtcaaggcc gagaagctgg tggactacac caacaagcag aacttcgccg agcgctttgc
     3961 caagcggctg ggggcgcagg tcggcgaggg ggcgctggcg gcgctgcgcg gcgcgccggg
     4021 ctggcgctga gcccaacaca ggcggccgtt gggcgcggcg caggcccgca gatggccggc
     4081 ggtcggccgc cgcgttccgg ccgaatcctc aggccgactg cgccagcggc agctccgcca
     4141 cgtcgaaacc ctgtggcggc agcccctggg cgtggcgctc gatcatgtgc tcgaaggcgc
     4201 gctccaggtg gcgggtgaag cggcgcgtgt cgaacaacgg gcgggtccgg atgttggcgg
     4261 ccagtcgatc gcgcaggccc tgcagcagcc ccggctcggt ggccagccga tgcgccagcg
     4321 cctcgtactc gggctggctg tgggtgatca gctcgggcag gccgatgcag tccagcaggc
     4381 tggcggccat gcggccggca aagcggtcgc cgacctgggt cagcaccggc agaccggccc
     4441 acagcgcatc gctgaccgtg gtgccgccgt tgaagggcag cgtgtccaga aacagatcgg
     4501 cctggcgata ccgggccagg tactggtcgt agggccgccg ttcggcgaac accaggcgct
     4561 cgggcgccac cccgcgcgcc tgcgcgtggg cgcgcaggtt gctgcgggcg gtgtcgttgt
     4621 cgcccagcag ccacagcacc gaaccgggtg tgttttgcag cagccgcatc cagccatcga
     4681 acacgccggg caggatcttg tagctgttgt tcaggcagca gaacacgaag ccctggtccg
     4741 gcaaccccag ttcccggcgg gtcggggttg cggccgcgaa gcggcgctgg tcgtcgttgg
     4801 cctggaagca gtcgggcatg cgcacgatgt gttcggaaaa cgcctgcgcc tgctcgaccg
     4861 gcgtgaccca gcggtcgcca aggatgtagt cgatcgccgc cagcccgctg gtgcccggga
     4921 agccgaggta ctgcacctgc accggcgctg gccggcgggc caggatgccg ggccgactga
     4981 aggtggtcag gccgttgaga tcgaccagca gatcgatgcc gtggccagcg atcagggcgg
     5041 cggcctcgtc atcgcccagg tggtggatgt cgatgaaatg atcgaaggcc gcctgcgcgc
     5101 gctgccgctc gggcgaccga tcgggttgcg ggtagatgga gaacgcgaag gtttcgaagc
     5161 gctggcggtc atgggcttcg aacacgccgg tcatcagaaa gccgaccgcg tggtgccgca
     5221 gatcggacga caggtaaccc agtcgcagcc ggcgcccggc cggtgcgcca cgcgccacct
     5281 tcgcgtgcat ggcgcgaaag gcgtcgttga gtggggcgtg atacgcctgc gcaatctgca
     5341 gcagatcggc ggcgctgacg tcggggtggg ccagcagcgt caacgtgttg gcgtcgcggg
     5401 caccggcgcg ccagtggcgc agcgtctgag ccaatacctc ggtcagacct tcccagtggg
     5461 cggtcttgag catgaagtac agcaactgca tggagacgga cagatcgtcg ggtgccagcg
     5521 catgggcttg cagcagcggc atcagcgccg ccgcgtcgcc gcgggtcttg gccagggcgg
     5581 ccgcctgggt cagcagcacg cggggcgact gcggcgccag gcgctgcgcc acctcgatgc
     5641 gttcgagcgc caggtcgggg cgcccaagct gcagcagcac gccgccgaac ttcagccagc
     5701 cgtccaggtt gtccggctcc agggccacga ctttctcgaa gcaggccagc gcgtccggca
     5761 ggcgctgcgc ctgctccagc gcgctgccca acaacagcca ggtgctggcc cgggggcgca
     5821 ggtcgatggc gcgctcgaag ctggcgatgg cgtcgcccag gcggtggagt tccagcaggg
     5881 catggccgcg gttgagccag gcgtcggcgt gccccggggc cagcgccagc gcccgatcgt
     5941 ggctggccag cgcctggagg tgctgaccca gtccgtgctg cacattgccc aggtcgtacc
     6001 agatgttggc gtcctggggt gccagcgcga gcgcctgacg gaagtggtcg atggcctgct
     6061 cggattgccc cagtcgggca agcaccgtgc cgagcaggca caggatctgg gtctgccgcg
     6121 ggttcagcgc cagcgcccgc tgcagcgccg gcagggcctc ggccgggcgg ttgccgacca
     6181 cgtgcaccat gcccagcagt tgccaggcat gggcgtggtt tggctgctgc tggagcacct
     6241 gctggcacaa gggggcggcc tgatccggct ggccgccgtt gaacaggcgc acggcgcggt
     6301 caaacagggc cggcacgccg ttggcggcgg acgatggcga ggggtgagct tgttgcttgg
     6361 acatggcggc gctgccgtct gggtgtgatg ggagggcggt tcggggcggc gacggcagcg
     6421 cctcaggggc cgatggcaaa caccgccggc tgagccgaca ggtctgccgt cagtgccgct
     6481 gaccgtaggc gccagtcggc cacgcgcagg ctggtgcagc gttgctgcgc cagcgtcagc
     6541 ccgctgcaca ccgccagccg ggtgtcgggt cggagctgct gcagcagggc ctgcagcagg
     6601 gctgcattgc ggtagggcgt ttcgatgaac atctggcttt ggccgctttg ctgtgcccag
     6661 gcctccagtt cgcgcaggcg cgtggcgcgg cggctggcct cctgcggcag atagcccacg
     6721 aacgcgaagc tctggccgtt cagcccgctg gcggccagcg ccagcagcat cgacaccgga
     6781 ccgaccaggg gccgcacctc gatgcccaga tcgtgggcgg cgcgcaccac cgaactgcca
     6841 gggtcggcca ccgcgggcat gccggcttcg ctgaccaagc ccaggtcgtg gccctgtagg
     6901 gccgaggcca gcagcgggcg ggcgtcccag gtgccggcgc gccggtgatc gccctgtttg
     6961 tggatggcgt gcggcagctg ctggatgtgc tgcgcctgca gcggttgcgc cagcggcacc
     7021 agcgcggcga cgcgcttgag aaaggcgcgg gtcgatctgg cgttctcgct gatccagtgc
     7081 gacaggcgcg cggcgacctc gatcgtgccc tggggcaggg cgtcggtcag gggcgcctgg
     7141 agggggcagc catggtccag cggcgttggc accaggtaca gcgtgccggg ggtcggggcc
     7201 gcgctcatgg cagccgcagc ccggcggcgc gcagcagccg ggcggtgcgg atcagcggca
     7261 ggccgaccag cgccgtgggg tcgtcgttgt ggatggcctc cagcagggcg atgcccaggc
     7321 cttcgctctt ggcgctgccg gcgcagtcgt agggttgttc ggcgcgcagg taacgctcga
     7381 tctcgcccgc gtccagcgcg cgaaagcgca cctcgaccgg cgccagctcg ctctgctcga
     7441 agccgctggc caggcacacc accgacaggg cggtctgaaa caccacggtc tgcccgctca
     7501 tgcgctgcaa ctgggccacg gcgcgttcgt ggctgccggg cttgcccagc ggctcgccgg
     7561 ccaggtcggc cacctggtcg gcaccgatga cgatggcctg cgggtggcgg gccgccactt
     7621 cgcgcgcctt ggccagggcc agccggcgcg ccagcgccgc cggtgtttcg tgggggagcg
     7681 gggtttcgtc cacctgcggc gcagcggtgg tgaacggcag gcgcaggcgc gacagcagtt
     7741 cgcggcgata gggggaggtg gagcccagca ccagcggtgg cgcggcgctg gcgtcggtgg
     7801 tgtcggtcgg catggcgccg attctcttac actggccgca tgagcaggag ctttgatgcg
     7861 cggcgcctcg acgtggcggc tttggccgag gccggggcca gcctttcggg cgccgatgag
     7921 ctacaaaatt atgagcgcct ggcgctcgaa gcgcaaggcg cggacaagga tctgacggtc
     7981 gaatggaagg tccagggcgg gcgccgtcag gcggtcgatg gccaatggcg gccgtcgctg
     8041 catctgcagg cgcagacacg cttgccgctg acctgtcagc gctgtctggg tccggtggcg
     8101 acgccgctgg tggtggaccg gcacttggta tttgttgaaa gcgaggcgcg cgccgccgct
     8161 ctggacgacg cgtccgacga cgacgtgctg gcgctgacac cggcgctcga tctgcgcgag
     8221 ctgatcgagg acgagctgct gctggcgctg ccgctggtgc cgcgtcacga ggtctgtccg
     8281 gacgccgtgc gtctgtcggc gcacagcgag gggttcgacg ccgcaccgcc gcagcggccc
     8341 catccctttg ctgcgctggc ggcgctgaag cagggcggca agggccgata gtgcgtgccg
     8401 ctgtggcgcg cccacgcttt cgcgctataa tttaaggctt cgcattcggc ggggcgcggc
     8461 cattggctgc cccaggccag gatgcatcgc ttacccactt gcccgcgtcc acgcccgagc
     8521 ggcggggccc atccaacaag gagccaccat ggctgttcag caaaacaaga agtcgccgtc
     8581 caagcgcggt atgcaccgtt cgcacaacgc gttgtccgtg cccggcatcg ctgtggaacc
     8641 gaccaccggc gaaacccacc tgcgccacca catcagcccc aacggcttct accgcggtcg
     8701 ccaggtcatc aagtccaaga ccgaagcctg atcgatggcg cgggcgcgcg gcgtgcgtgc
     8761 gaccgtcgcc gttttccatc cgccgatgac tccctggccc ggcgctacga cccgtagcct
     8821 cgggccttcg gcgtttgcgc gtccgggcga tccagcccgg ttcatggttc gagccgtgcc
     8881 gtcgcgcgac ggcggcggcg ctgttgcggg gcttgcatga tcgttctggc cgtcgattgc
     8941 atggggggtg accacggccc cgcggtcacc ttgccggcgt gccggcagtt tctcgaccgc
     9001 caccccgagg ccgagctgct gctggtcggc cagccggagg cgctggcggg cctgacgcac
     9061 gagcgcgccc gggtgatcgc cgccagcgaa gtggtgacca tggacgacgc ggtggaggtg
     9121 gccctgcgtc gcaagaagga ttcgtccatg cgcgtggcca tccagcaggt caaggacggc
     9181 gcggcccagg cggcggtgtc ggccggcaac accggcgcgc tgatggcggt ggcgcgctac
     9241 ctgctcaaga cgctggaggg catcgaccgc ccggccatcg ccacccagat tcccaaccag
     9301 aagggtggcg ccaccaccgt gctcgacctg ggcgccaacg tggactgcac ggccgagcac
     9361 ctgctgcagt tcgccgtcat gggctcggcc ctggtgtcgg tgctcaacga cgacccgcag
     9421 cccacggtcg gcctgctcaa catcggtgaa gaggccatca agggcagcga ggagatcaag
     9481 cgcgccggcg aactgctgcg cgctgcgggc gatgccggcg acctgaactt cgtcggcaac
     9541 gtcgagggca acgacatctt caagggcacg gccgacatcg tggtgtgcga cggtttcgtc
     9601 ggcaacgtgg cgctcaagac catcgagggg ctggccagca tgatcggcgg cttcatgaag
     9661 gaagagttct cgcgcaacct gctgaccaag gcggcggccg tcgtcgccta tccggtgctg
     9721 gccgcgctca agcgccgcat ggaccaccgg cgctacaacg gcgccgcgct gctggggctg
     9781 cgcggtctgg tgttcaagag ccatggttcg gccgacgcgc tggccttcga gcaggcgctg
     9841 gtgcgcgcgt atgatgccgc cagccatcaa ttgctggcgc gcgtgcagca gcgcatcgcc
     9901 catgcggcgc cgcttctggc ggcgtccggg gcggctgcag cgccggctgc ctgagcccgg
     9961 cgcggccgcg gcccggcatc cacacaagca tgttcatgag cagacgctat tcccgcatca
    10021 ccggcaccgg cagctacctg ccgccgcgcc gcgtcaccaa cgcggatctg gtggccgagc
    10081 tggccagccg cggcatcgag acttcggacg aatggatcgt cgagcgcacc ggcatccgtg
    10141 cgcgccatct ggcggcgccc gacgtgcccg ccagcgagct ggcggtgccc gcggcgcggc
    10201 aggcgctggc ggcggcgggg ctgcagccgg gagacatcga cctcatcatc gtcgccacct
    10261 cgacgcccga catggtgttc ccgtccaccg ccgccatcgt gcagcacaag ctgggcatgg
    10321 ccggctgccc ggtgttcgac gtgcaggcgg tgtgcagcgg cttcgtctac gcgctcacgg
    10381 tggccgatgc gctgatcgcc aatggcacgg cgcgccgcgc gctggtgatc ggcgccgagg
    10441 tgttttcgcg catcctcgac tggagcgacc gcaccacctg cgtgctgttc ggtgacggcg
    10501 ctggcgccgt ggtgctggaa gccagcgacc ggcccggcat cctggccacc gacctgcacg
    10561 ccgatggcaa gtacgtcgac atcctgtgcg tgcccggtgg cgtctcgggc gggcaggtgc
    10621 tgggcgatcc gctgctcaag atggacggcc aggcggtgtt caagctggcc gtcggcgtgc
    10681 tcgacaaggc cgcccgcgcc gtgctggcca aggccggcaa gagcgaggcc gacatcgact
    10741 ggctgatccc gcaccaggcc aacctgcgca tcatgcacgg caccgccaga aagctgcacc
    10801 tgccgctgga caaggtgatc gtcaccgtga acgagcacgg caacacctcg gcggcgtcga
    10861 ttccgctggc gcttgaccac ggcgtgcgca gcgggcagat tcagcccggc cagacgctgc
    10921 tgctggaagg cgtgggcggt ggcttcacct ggggtgcggt gctcctcaat atgtagctgc
    10981 aggcgcttgc tggataagcg ctgggatcgt ttttcttcat ttttcctgag cccatcatga
    11041 aaccctttgc tttcgtcttt cctggccagg gctcgcaggc cgtgggcatg ctggatgcct
    11101 ggggcgacca cccggcggtg cgcgacacgc tggccgacgc ctcggccgcg ctgggcgaag
    11161 acctgggcgc actgatcgcc cagggtccca aggaggcgct ggcgctcacc accaacaccc
    11221 agccggtgat gctggtggcc ggcgtggcct gctggcgcgc ctggcgcgcc gagggcgggg
    11281 tcgctccggc agcggtggcc gggcattcgc tgggcgagta ctcggccctg gtggccgccg
    11341 aggtcctgac gctggcccag gccgtgccgc tggtgcgcct gcgcgccgcc gccatgcagc
    11401 aggcggtgcc ggtgggcacg ggcgccatgg ccgccattct ggggctggat gctcaaaaag
    11461 tgatcgccgg ctgcgccgaa gccacggccg ccatgggcca ggcgggcgcc gaagtggtgg
    11521 aagcggtcaa tttcaatgac ccggcgcaga ccgtgatcgc cggcagcaag gcggcggtcg
    11581 acaaggcctg cgaggtgctc aagggcctgg gcgccaagcg cgcgctgcca ctgccggtgt
    11641 cggcgccgtt tcactccagc ctgatgaagc cggccgccga gcagctgaag gcggcgctgg
    11701 cggcgctcga attgggcgcg ccgcgcatcc cggtggtcaa caacatcgac gtcgcggtcg
    11761 aacaggaggc ggaccgcatc cgcgacgcgc tctaccgcca ggccttcggg ccggtgcgct
    11821 gggtcgagtg cgtgcagaag atcagggcgc tgggcatcga caccgtggtc gaatgcggcc
    11881 ccggcaaggt gctggccggc atgaccaagc gcatcgacgc cggtctgacc ggcgccgccc
    11941 tgtacgaccc ggccacgctg gccgaagtca aggcgctgct gggctgaagc cgcacgccac
    12001 gaggagaccc atcacatgtc agacacccca ttcgcaggcc aggtggccct ggtcacgggc
    12061 gcctcgcgcg gcattggcgc ggccatcgcg gcgcaactgg ccgagcgcgg cctcaaggtc
    12121 atcggcaccg ccaccaccga agacggggcg gcgcgcatcg gcgccgcgct ggcgtcgcac
    12181 cccggctgcc gcggcgccgc gctcgacgtc aacgacgccg ccgcggccga agcgctgatc
    12241 gaggccatcg tcaaggaaca cggcgcgttg cacgtgctgg tcaacaacgc cggcatcacg
    12301 cgcgacatgc tggccatgcg gctgaaggac gaggactggg acgcggtgct ggacaccaac
    12361 ctgaaggcgg tgtttcgcat gagccgcgcc gtcatgcggc ccatgatgaa gcagcgctat
    12421 ggccgcatcg tcaacatcac cagcgtagtc ggcgcttcgg gcaacccggg gcaggccaac
    12481 tacgccgccg ccaaggccgg cgtggccggc atgacgcgcg cgctggcgcg cgagctgggc
    12541 agccgcggca tcaccgtcaa ctgcgtggcg cccggtttca tcgccaccga catgaccgcc
    12601 gccctgggcg aggaacagca caaggcgctg gcggcgcaga ttccgctcgg ccgcctgggt
    12661 cagccggccg acattgccgc cgccgtggcc tacctggcca gccccgaggc gggctacgtc
    12721 acaggtcagg agctgcacgt caacggcggc atgttcatgg cctgagccat taccatcggc
    12781 cactggccgt cgcctgtcgc gtctgctccg gtctgcgggg cggcgcggct agaatccacc
    12841 atttcttact agccccagag ggaacccatg agcgatatcg aagcacgtgt caagaaaatc
    12901 atcgccgagc agctcggcgt ggaggagtca caggtcacca acgaaaaagc cttcgtggcc
    12961 gacctgggcg ccgattcgct cgacacggtt gaactggtga tggcgctgga agacgagttc
    13021 ggcatcgaaa tccccgacga ggacgccgaa aagatcacca ccgtgcaggc ggccatcgac
    13081 tacgccgtca gcaagaaggc gtaaccccca ccgcggtttt gccgccggcg tgggcgcgac
    13141 ccccagcggc cggcccgcgc cacgcagcgc ttcgtggccg agcgcgtgcg gtcggacggt
    13201 ctgagccgtc ttgcacgcac cgtccggcgg agcacgacca cagacagaca agcaagatcg
    13261 gatgaccatg aatcgacgcc gtgttgtcgt caccggcctg ggttgcgtca gccccgtggg
    13321 caacacggtg gccgaaagct gggccagcct gctggccggc cgttcgggca tcgacctcat
    13381 cacgcgcttt gacgccagcg ccttcgccag ccgcattgcc ggcgaggtcc gggggctgga
    13441 catttcgccg catatctcgg cgaaagaggc gcgcaccatg gataccttca tccactacgg
    13501 catcgtggcc gccgccgagg cagtgcagga cgccggcctg gccacgggcg aggcgctgga
    13561 cgacgaggcc gccacgcgca tcggctgcgt ggttggctcg ggcatcggcg gcctgccgct
    13621 gatcgaagag acccagaccg agtacgccgc ccgcggtccg cgccgcattt cgccgttttt
    13681 cgtgccagcg tccatcgtca acatggtggc tggtcatgtg tcgatgaagt tcggcttcaa
    13741 ggggcccaac ctggcgatcg ccaccgcctg caccaccggc ctgcactgca tcggcgaggc
    13801 cgggcgcctg atcgagtacg gcgacgccga tgtgatgatt gccggcggcg ccgaatccac
    13861 ggtgtcgccg ctgggcgtgg gcggttttgc tgccatgcgc gcgctgtcca cccgcaacga
    13921 cgaccccaag accgcctcgc gcccctggga caaggaccgc gacggcttcg tgctgggcga
    13981 gggtgccggc atcctggtgc tcgaggcgta cgaacacgcc aaggcgcgcg gcgcccgcat
    14041 ctatgccgaa ctggcgggct tcggcatgag cgccgacgcc ggccacatga ccgcccccaa
    14101 catggacggc ccgcgccgcg ccatggtggc tgccctgcgc aacgccggcc tgaacgccga
    14161 ccaggtgcag tacgtcaacg cccacggcac ctccaccccg ctgggcgaca tcaacgaatc
    14221 caacgccatc aaggcggcgc tgggcgatgc cgcccggcgc gcggtggtca gctccaccaa
    14281 gtccatgacc ggccacctgc tgggcggcgc cggcggcatc gaaagcgtgt tcagcgtgct
    14341 ggccctgcac caccagaagg tgccgcccac catcaatctt ttcaacccgg atcccgagtg
    14401 cgacctggac tactgcgcca acagcgcgcg cgacatgaag atcgacgcgg tgctgaagaa
    14461 caacttcggc tttggcggca ccaacggttc gctggtgttc aggcgccttt gattgcgctg
    14521 cagcgcgcgc tgggcgacgc ccgacgtctc cgcccggcgg cgcctgaccg acagcccacg
    14581 tggcccacgc ccctgccgtg agttatccgg tcggccgcag cgccgcgctg gcgcgcgtgc
    14641 tgctgggcct ggcggcggcc ggttggctgg ccctgggttt gacatatttc ttggctccag
    14701 cgcttatcca gcttgcgcca gcagctatgc taagcatagc aatcaatgcg ctggtgacgc
    14761 ttgggctgtg gcgcttctgg cgcgcgcagg aggccaggca gctgcgctgg accggcgaac
    14821 actggctgct gcacacccct ggcaccgagc cggaccgggg cggcgagggc accggtgtcg
    14881 aggtgcggct ggacgcccag cgctggctgc tgctgcgctt tcgcgacccg gccgaagcgg
    14941 gtgcgcactg gctgtgggcc caggcttccc acgatccggc gcgctggcac ctgctgcgct
    15001 gtgcgctata ttcggccgcg ccttcgcccg ccgcgaccca ggcttcgcgc ccctgaccac
    15061 cggcgcgccg cctgtgctgg acccctgttc gtgaccgatt ccatcgcccc cccgcctgcg
    15121 cccgccgaca gcgatgcgct gctggtcgag cgcgcggcgg caggcgacac gcgcgcctac
    15181 gagctgctgg tcatcaagta ccagcgccgc atcgagcgcc tgatcgggcg cctggtgcgc
    15241 gacgtcgatc tggtgcccga catcgcgcag gaaacctttc tgcgcgcctg gcgggcgctg
    15301 ccgcagtttc gcggggaagc ccagttctac acctggctgt accgcatcgc ggtcaacacg
    15361 gccaagaagt cgctgctgga gctcaagcgc gatccggtgc tgaccgagtc ggccctgcac
    15421 accagcgacg acgacgatga aacttcccgg cgggagaacg aactaatctc cgagtccacg
    15481 ccggagagcg aactggcggc acgtgaaatc gccgccgccg tcaatgccgc cgtcgaggcc
    15541 ctgcccgagg atctgcgcca ggccatcacg ctgcgcgaga tcgagggcct gagctatgaa
    15601 gagattgcac acaccatgaa ctgcccgatc ggtaccgtcc gctcgcgcat cttccgcgca
    15661 cgcgaagcga tttcggctcg ggtcaagccc ctgctgagtc accagaccgg caagcgctgg
    15721 tgacggcaac ccctgcaatc ggtgatcacc catgaacgct cgaatcgatc aaacgctgga
    15781 tctggaagcc atctcggccc tgatggacgg gcagctgcgc gatgacgacg cagcccgggc
    15841 cctcggcatg atgaccgcct ccggccaggc gcgtgaatcc tggcagctgt atcacctggt
    15901 gggcgatgtg ctgcgctcgg ccgatctggc cgcctgcggg caggatcggg ccttcgcggc
    15961 acgccttgcc gcaacgctgg cggcgcagcg accggccgag gtcgccgcgc cgcgggcgcc
    16021 ggccctgtcg ccgcatgcgc tcacgcgacc ggcggccaac gacggcctgt ttggctggcg
    16081 catggtggcc ggactggcct cgtttgccgc cgtggccgtg atcggctggg gaatgctggg
    16141 cgatctcgga ccccagcgtc agggcggggc gcagctggcg cagggcgctg ccgcgccgcg
    16201 gaccgcgctg cagaccgtgg ccggtccgtc gcctgcgctg agcaccgccg agccggatga
    16261 aatccgggtg gccgacgggc cggcggtgat gttgcgcgat ccgcgcctcg atgaactgct
    16321 gcaggcgcat cgccaggtgt cggcaacgcc ggcgctgggc ggtttcgtgc tcaacgccac
    16381 ctacgagggt gcagggcgtt gaaggcgatc gggtggggtc gggtccgcgg cggtttgcgc
    16441 cgcgctgcgc tgctgtggtg tgtcggcggc atggtcagtc tggcgggcgc gcagagcatc
    16501 ggccagttcg gcggcggtgc gcccgccggg gtggttggtc cgtcgcagcg cagcgtggcc
    16561 gagtggctgg ccaggctgca acaggggtct cgggtgcctt cctacatcgg caccttcgtc
    16621 gtgtcgtcgg cgtctggcgc catgtccagc tcacgcatct ggcatgtgtg cgagggcgat
    16681 gtgcaattgg agcgcgtcga tgccctgacc ggcgtgccgc gttccaccct gcggcgcaac
    16741 gacacggtga tgaccttcct gcccgatgcg cgggtggtca cgctggatca gcgccagctg
    16801 ggtgccgtgt tccccaacct gctcaatctg gcgcaggggc tgccggtcgc cgactactat
    16861 gacgcgcgtg aactggggcg cggccgcgtg gccggctgcg atgccgagat cgtgcagctg
    16921 ctgccgcgcg acgaatggcg ctacggctac cgcatctgga gcgaacggcg caccggcctg
    16981 gtgctcaaga cccagaccct gaacgcccac ggccgggtgc tggagcaggc cgccttttcc
    17041 gaggtgcagc tggacgcgcc ggtgcaggcc gagcagctgc gccagatgat ggccaacacc
    17101 cagggctacc gcatcgtcaa ggccgagcac gccaggacca cgccgcaggc cgaaggctgg
    17161 cagctgacga ccatggtgcc cggtttcgag gcgcgcaact gctaccgccg tccgccttcg
    17221 gccagcgcat cgctggtgca gtgcctgttt tcggatgggc tggccacggt gtcgctgttc
    17281 atgcaaccgc tcgatccggc gcggcaccag caggaaggcg cggtcgccat gggcgctact
    17341 cagaccctga cgcgccgcgt ggtcgaccgc tccggtcagt ggtgggtcac cgccgtcggc
    17401 gaagtcccgc cagccaccct gcaggccttc gtccgcgggc tggaacgcat tccctgagcg
    17461 cggcgttcac ccaccccgcc gcccacgcaa cgatcaccga catgcctttg ctgaacatga
    17521 acaagttgtc cctggccgcg ctggccgtcc cgatgctgct gagcgccacc ctgaatctgc
    17581 cgctggccgc gcaggcccag gcgcccgcgg cacccatcgt gcgtgggctg ccggacttca
    17641 ccgatctggt cgagcaggtg ggcccggcgg tggtcaacat ccgcaccgtc gagcgcgtgc
    17701 gcaccggcat cgggcgcaat ggcccgatga tggatgagga gatgcaggag ttcttccgac
    17761 gcttcttcgg cctgccgctg cccggcgtgc cgcgccaggg cccgcgccag caggagccgc
    17821 aggagccggc cgagcgcgag gtgccgcgcg gcgtggggtc cggcttcatc ctcaccgccg
    17881 acggcttcgt gatgaccaac gcccatgtgg tggacggcgc cgacgaagtg ctggtgacgc
    17941 tgaccgacaa gcgcgaattc aaggctcgca tcgtcggtgc cgacaagcgc accgacgtgg
    18001 ccgtggtcaa gatcgaggcc agcggcctgc cggccgtgcg catcggtgac gtcagccgcc
    18061 tgaaggtggg cgaatgggtg atggccatcg gctcgccctt cggtctcgac aacaccgtga
    18121 cagccggcat cgtcagtgcc aagcagcgcg acacgggcga ctacctgccc ttcatccaga
    18181 ccgacgtggc ggtcaacccg ggcaactcgg gcgggccact gatcaacatg cgcggtgagg
    18241 tggtgggcat caacagccag atctactcgc gctccggcgg cttcatgggc atttcgtttg
    18301 ccatcccgat cgacgaggcc atgcgcgtga gcgagcaact gcgcgccagc ggtcgcgtca
    18361 cgcgcggccg cattggggtg cagatcgatc aggtgagcaa ggaggtggcc gagtccatcg
    18421 gcctgggcaa gccgcagggc gcgctggtgc gtggcgtcga atccggctcg ccggccgaca
    18481 aggcgggcgt cgaggcgggc gacatcatca cccgcttcga tggccgggtg atcgataaag
    18541 cgtccgatct gccgcgccta gtgggcaaca ccaaacccgg cacgcgcagc gccctcacgg
    18601 tgttccggcg cggccagacg cgcgagctga acatcaccat cgccgagctg gaacctgagc
    18661 agaccgcgcg ccgacccagc cagcgcggca gcgccccacc caaggcctct gaagccaccc
    18721 aggtgctcgg cctgaccgtc accgaactca ccgaagccca gaagaaggag ctgaaggtgc
    18781 gccacggcgt gcgcgtggaa gcggccaccg acgccggtgc gcgcgccggc ctgcgcgaag
    18841 gcgacgtgat cctgtcggtc ggcaacgccg aggtccgcag tgtgcgggac ttcgaggcgg
    18901 cggtggccaa ggccgagcgc ggcaaaccca tcagcatgct ggtgcagcgc ggcgagctgg
    18961 tgcagttcgt gctggtcagg cctggtcgct gagcctgcct gggccggggc ataacccgaa
    19021 actgaggtcg ggtatggggc tattcggggt gctgacggcc gccccgaggc ggcaggccag
    19081 tcgggcaggc cgcctgtggc accgaagtta catacgcgtt tggatagaat acgggtcggt
    19141 caaaggtcgt gattcgcata tgatccggcc gtcacaccgc catgtgggac gcggttggat
    19201 gatccaccgg atttccacaa gccgctgaaa gttgttcaga aattatcctg caaatcatgt
    19261 tgacaacctg ttgataaata ttcgttgcag cgtcacaacg tggcggagat gagattccgt
    19321 atcgcttggc cgggtggttt ttgcctacaa tgaagtccca accatcgcag ttgccataga
    19381 ttgttggttt gagggcgcgc cgcatcactg gcgcgccctt ttttcatgtg cgcatctcgg
    19441 taattcaaat acttgccttc tttcgttgat ggaccacatc agaaatttct caatcattgc
    19501 gcacatcgat cacggcaagt ccacgcttgc cgatcggctc atctcgcgtt gcggcggcct
    19561 gtccgaccgt gagatgagcg agcaggtgct ggactcgatg gacatcgaga aggagcgggg
    19621 gataaccatc aaggcccaga ccgccgcgct gcaataccaa gcgcgcgatg gcagggtcta
    19681 caacctcaac ctgatcgaca ccccgggcca tgtggacttc tcctacgagg tgtcgcgttc
    19741 gctgtcggcg tgcgaagggg cgctgctggt ggtcgatgcc agccagggcg tggaggcgca
    19801 gacggtggcc aactgctaca ccgcactgga gctcggcgtg gaagtgctgc cggtgctcaa
    19861 caagatggac ctgcctcagg ccgacccgga caacgccaag gccgagatcg aggacgtgat
    19921 cggcatcgat gccagcgagg ccatcgccat ctcggccaag accggcatgg gcatcgacga
    19981 cgtgctggaa cagatcgtcg ccaaggtgcc gccaccgcgc ggccaggccc aggcgccgct
    20041 gcgcgccatg atcatcgatt cctggtttga cgcctacgtc ggcgtggtga tgctggtgcg
    20101 cgtggtcgac ggccgactgg ccaagggcga gcgcttcagg atgatggcca gcggctcggc
    20161 ctatgaagcc aaccagctcg gcgtgttcac cccggcgcag caggcgcgtg aggtgctgga
    20221 agcgggcgag gtcggctaca tcatcgccgg catcaaggag ctcaaggccg ccaaggtggg
    20281 cgacaccatc acgctggaga agaagctgcc gggcaacctg ggcccggccg acaggccgct
    20341 gccgggcttc aaggagatcc agccgcaggt gtttgccggc ctgtacccga ccgaggccaa
    20401 ccagtacgac cagctgcgcg acgcgctgga aaagctccag ctcaacgacg cctcgctgca
    20461 cttcgagccg gaggtcagcc aggcgctggg cttcggcttt cgctgtggct ttctgggcct
    20521 gctgcacatg gagatcgtgc aggagcgcct ggagcgcgag ttcgatcagg acctgatcac
    20581 caccgcgccc agcgtggtgt acgaggtgga aaaggccgac ggcgaggtga tccaggtcga
    20641 gaacccctcg aagatgcccg accagggcaa gatccacgag atccgtgagc ccatcgtcac
    20701 ggtgcacctg tacatgccgc aggactatgt gggtccggtg atgacgctgg ccaaccagaa
    20761 gcgcggcgtg cagatcaaca tgcagtacca cggccgccag gtcatgctga cctacgagct
    20821 gccgctgggc gagatcgtgc tcgacttctt cgacaagctc aagagcgtca gccgcggcta
    20881 tgcctcgatg gactacgagt tcaaggaata tcgcccctcc gacgtggtca aggtcgacat
    20941 cctgctcaac ggcgaaaagg tcgatgcgct gtcgatcatc gtgcaccgca gtcaggcgca
    21001 gttccgcggc cgggcggtgg cggccaagat gcgcgagatc atcagccgcc agcagttcga
    21061 cgtggcgatc caggcggcca ttggtgccaa catcatcgcg cgcgagaaca tcaaggcact
    21121 gcgcaagaac gtgctggcaa aatgctatgg gggtgacatc acccgcaagc gcaaactatt
    21181 ggaaaaacag aaggcgggca agaagagaat gaagcagatc ggttcggtcg aggtgccgca
    21241 ggaggcattc ctcgccatct tgcaggtgga ggactgatgc ccttgctgac cgccgtcatt
    21301 ctggccgcct tcgtggccta cgccgccgcc tggtacgtcg gcatccttca gggcaacttc
    21361 gcgttgctgc tgctgctggc cacgctggtg accggtgcgt actggctggg cgagcgcttc
    21421 gtgtttctgc cgcgccgtcg tgccgccgcc caggccctgg aggcgcaggc ggcccagcgc
    21481 cgcagcgagc tggcggccca gggcatcacc caggtggacg agggcgagct gacccaggcg
    21541 cgcgagcgcg tgctggccca gccctggtgg ctggactgga cggccggcct gttcccggtg
    21601 attgcggtgg tgtttctgct gcgctcgttc gtgttcgagc cgttcaagat tccctccggc
    21661 tccatggtgc cgaccctgct ggtgggcgat ctgattctgg tgaacaagta cacctatggc
    21721 ctgaagctgc cggtgatcca cacccgcctg actcagggcc agccggtgca gcgcggcgac
    21781 gtggtggtgt tccgctatcc accgcagccc agcgtggact acatcaagcg cgtggtcggc
    21841 ctgcccggcg acgaggtggc ctacctcaac aagcagctca ccatcaatgg ccagccggtg
    21901 ccgcagacgg cggtgtccga cttcttcgac gaacaggcga tggaatactt caagcagttc
    21961 gacgaacgcc tgggcgtgac aaatcaccgg atcatcgtcg acgggcggcg tccggctttc
    22021 gtgggcgggg cttcggactt cgaatacaag gacaattgcc gctacagcgt cgagggtgtc
    22081 cgctgcaagg tgccgcccgg gcattacttc atgatgggcg acaaccgcga caattcgctc
    22141 gattcgcgct tctggggctt cgtgcctgac ggccacatcg tcggcaaggc gttcttcgtc
    22201 tggatgaact tcggcaacct caagcgcatt ggctccttcc attgatcctt tccgaccctt
    22261 cgcgagaaaa atgaaacatc aaggtccatc gtcccagcgt ggcatttcct ttttcggcct
    22321 gctgttcctc ggcgtcgtgc tggcgctgct ggccatcgtc ggtgcacggg tggtgcccac
    22381 ggcgtccgag ttcatgacga tccagaaggc ggtcaagaaa gccgccgccg acggcgacac
    22441 cgtgccggcc gtgcgcgcct cgttcgaccg caccgcctcg gtggactaca tcacctccat
    22501 ctccggcaag gacctggagg tcaccaagca gggcgacaag gtggtggtca acttcgccta
    22561 cgacaaggaa atcccgctgt ttggtccggc ctacctgctg atcaagtacc gtggctcctc
    22621 cgcgggtggc tacgactgag cgtccgccga cgtccgcgcc cggctcgtcg ggtcgcgcac
    22681 gtgcgccctg gaaggccctg caggagcgtc tgcagtacca ctttcgggat gagtcgctgc
    22741 tcgagcgggc gctgacgcac cgcagcttct cggccgacca caacgagcgc ctggagttcc
    22801 tgggtgactc ggtgctgggt ctgggtgtgg cccacatgct ttaccaggcg ctcggcgggg
    22861 gtgccgaagg cgatctgtcg cgcctgcgcg cccagctggt gcggcaggac agcctgcatc
    22921 agctggcgct ccacctgggt ctgccgccac tgttgcgcct gggcgagggc gagctgcgct
    22981 cgggcgggca gcgccggccc tcgatcctgg ccgacgcgct ggaggccctg atcggcgccg
    23041 tctacctgga cggcggccac gcgcccgccg atgcgctggt gcgccggctg ttcgagcagg
    23101 tggagatttc ccccgccatg tcggccgccg ccaaggacgc caagaccgcc ttgcaggaat
    23161 ggctgcaggc gcgcaagatg cgcttgcccc agtacgaggt ggtgcgcgtg ctcggcgccg
    23221 cgcaccgtca gaccttcgag gtgcgctgcc aggtggccga actgaacctg gaagtgctcg
    23281 ggcagggcgc ctcgcgccgt gccgccgagc agtgcgccgc cgcgtcgatg ctggagcaac
    23341 tccagaaccg tccatgaata ctcctgaaat catagctgac ggcgctgatc cagagcgcgc
    23401 tggggcccaa tctggcactc catccgcgcc cgattccggg gccagcgtgc agcgctgcgg
    23461 cctgatcgcc atcgtcggca agcccaacgt cggcaagtcg accctgctca acgccctggt
    23521 ggggcagaag atcagcatca ccagccgcaa ggcgcagacc acgcgccacc gcatcaccgg
    23581 cgtgcgcacg ctgaccgacg ggcagggcgg tggcacgcag tatgtgttcg tcgacacccc
    23641 gggctttcag acccggcaca gcacggcgct caacaagtcg ctcaacaaga cggtgctggg
    23701 cgccatcgac ggggtggatc tggtgctgtt cgtggtcgag gccggcagct tcaccctgca
    23761 ggacgccaag gtgctgtcgc tgctcaagcc cggcacgccg gcgctgctgg tggccaacaa
    23821 gctcgacagc gtgcaccgcc gcgccgagat cgcgccctgg ctcaagggca tgcaggagcg
    23881 ccacccattc gccgagttcg tgcccatgtc ggccaagaac ccgcgcgacg tggcgcgcct
    23941 gctggagatc tgcgcgcgtt acctgcccga gcagccctgg tggtacggcg aagacgaact
    24001 gaccgaccgc agcgaaaaat tcctggccag cgaaacggtg cgcgaaaagc tgtttcgcct
    24061 caccggcgac gagctgccct acacctccac cgtggtgatc gagcagttca aggaagaaaa
    24121 aagccgtgcc cacggccgcc tgctgcgcat cgccgccacc atcgtggtcg agcgcgaagg
    24181 ccacaaggcg atggtcatcg gccagcaggg cgagcgcctc aagcgcatcg gcaccgaagc
    24241 gcgccaggag ctcgaaaagc tgctggacgc caaggtgttc ctggaaatct gggtcaaggt
    24301 gcgctccggc tgggccgacg acgaggcgcg cgtgcgcagc ttcggctatg aatgacagat
    24361 gacgaatgac ctcgcctgcg gcttcgatgg cgaatgcgcg aatggccggc cgtcgtctgt
    24421 ccgaagtcaa tcgtcattgg catgaagcgc gtcgttcacg agcccggcta cgtgctgcac
    24481 cactacgact ggagcgaatc cagcctgatc gtcgaggcct tcacgcgcgc gcacggccgc
    24541 gtggccctgg tggccaaggg cgccaagcgg ccgtcgtcca acttccggcc ggtgctgctg
    24601 ccgctgcagc cgctgctgct gtcctggggt ggtgacggcg agatccgcac gctcaaggtg
    24661 gccgagtggg gcggcgggca ggtgatgccg cgcggcgatg cgctgctgtc gggcttttac
    24721 gccaacgagc tgctgctgcg cctgctggcg cgcgaggatg cccacccgcg cctgttcgac
    24781 gcctatgccg ccctggtgca tgcgctggcg gcgggcgatg ccgagccgct ggcggccgcc
    24841 gcgctgcgcg ccttcgagct gctgctgctg cgcgaaaccg gccacctgcc agcgctcgac
    24901 gcccagaccc tgaccctggc gccgctgcag gcgagcgagc cctaccggct ggtggccgaa
    24961 ggcggcctgc gcgccgccca cgccgacgac gcggcctggc tgccgggcca gagctggcga
    25021 cgactggccg cctccctggc ggacgaagcc ccctggaccg ccaccctgac ggtggtggtc
    25081 ggcctgccgg gtgaacagcg cggcagcctg cgccgccagc ttggcgagct gctccattac
    25141 catggcggcg gtgccacgtt gcgcacgcgc cagcttctgc aggacgccca gcgcctgatg
    25201 gcgacctgag acgcgcagcc accgagcccg ccatgcccac ccagctttcc gtcaacgtca
    25261 acaaggtcgc cctggtgcgc aacacgcgcc acctgggcat tcccagcgtc acccgcgccg
    25321 cccagctgtg cctgcaggcg ggcgcgcacg gcatcaccgt gcacccgcgc ccggacgcgc
    25381 gccacatccg cgccgacgac gtgcatgcgc tggcggcgct gatgcgcggc tggccggggc
    25441 gtgaattcaa catcgagggc aacccgacgc agaacctgat ggacttcatc cgcgccgtgc
    25501 gcccggcgca ggccaccttc gtgcccgaca gcgaagacca gttcaccagc gaccacggct
    25561 gggacctggc cgacgccggc gtggcggccc gcctgcggcc gctgatcgac gaatgccagg
    25621 gcctgggcgt gcgcgtgagc ctgttcatgg acccgctgcc cgaactgatg gcggccgcgc
    25681 gcgcggtcgg tgccgagcgc gtggagctct acaccgagcc ctacgccgcc gcctggggta
    25741 cgcccgaaca ggggcgccag ctgcagcgct acgccgtcgc ggcgcaggcg gcaggcgacg
    25801 ccggcctggg cgtcaacgcc gggcacgatc tgaacctgca caacctgggc accttcgtgg
    25861 cgcgcgtgcc ggggctgctg gaagtgtcga tcggccacgc cttcatcgcc gacgcgctgg
    25921 agcgcggcta cgccgccacc accgaggcct acctgagctg catcgcccag gccggacgct
    25981 cctgaatgaa tagcttgcga cgcttgctgc atgctcgttg caggccactt caagcttgat
    26041 cccatgatcc acggcatcgg caccgacatc tgcgacgtgc gccgcatccg cgccagcgtc
    26101 gagcgccacg gcgagcgctt tgcgcagaag atcctgagcg aggccgaact ggccgcgtgg
    26161 cgccgccgca gcgcccgctg gcccgagcgc gggctgcgtt acctggccac acgcttttcg
    26221 gccaaggaag cgttttccaa ggcggtcggc ctgggcatgc gcatgcccat gcactggcgc
    26281 cgctgcgaaa tcgccaacca gcccagcggc cagccgtgca tcgtgttgca cggaccgctg
    26341 aaggactggt tcgaggcccg gcacctggtg gcgcacatca gcgtgagcga cgagagcgac
    26401 tacgcggcca gcttctgcgt ggtcgagcgc ctgccgccgg ttcgtgaatg aaaagcggct
    26461 ctggcgcgct cccggcaagc gccagaagct atgataattg tagtcaccga gcttaccgaa
    26521 aggccccatg cagcacgccc ccctgatcat cgacatcgcc ggcaccgccc tgaccgagga
    26581 cgatggtcgc cgcctggcgc accccctggt gggcggcctg atcctgtttg cccgcaactg
    26641 gcaggatcgt gaacagctcg gcagcctgtg cgccgaagtc aggcgcctgc ggcccgaact
    26701 gctgatctgc gtcgatcacg aaggcgggcg cgtgcagcgc tttcgcagcg gcggcttcac
    26761 ccacctgccg cccatgcgcg cgctgggcga gctgtggatg caggacgcca tgcgcgcgca
    26821 ggacgcggcc agcgcctgcg gctacgtgct gggcgccgaa ctgcgcgcct gcggcgtcga
    26881 tctgtcgttc accccggtgc tggacctgga ctggggcccc tcgggggtga tcggcgaccg
    26941 cgctttccat gccgacgccc gcgtcgtcac catgctggcc aagagcctgg cgcacggcct
    27001 gcggcgtgcc ggcatggcct gctgcggcaa gcatttcccg gggcatggct tcgtcgccgc
    27061 cgattcgcac accgcgttgc cgcgcgacac gcgcacgctc aaggccatcc tggccgccga
    27121 cgccgcgccc tacggctggc tgagcagcgt gctggacgcg gtgatggccg cccacgtcgt
    27181 ctatccgcgg gtggacgcgc ggccggccgg ctattcggcg cgctggctgc aggcggtgct
    27241 gcgcgggcgg ctggggtttg gcggcgccat cttcaccgac gacctcagca tgcaagccgc
    27301 gcgccacctc gaaggtcgca ccgtcacccc ggtcgaggcg gtgctggccg cgctcgacgc
    27361 cggctgcgac ctggcgctgc tgtgcaacca gtcgctcggt ggcggcgcgg tgctggacga
    27421 agtgatcgac ggcctggcgc gcgcccaggt tcagggccgc tggcagccgc gcgaggccag
    27481 cgccgcgcgc tgccaggcgc tgctgccgcg cgagccggcg cccgactggg acgcgctgat
    27541 gcgctcgccc gcctacctgc acgcgctggc gctggttccc tgaacccgcc acgcggcggc
    27601 tggcggcccg cccgctgcgg gcggttgtcg gggctcagac gagcgcggtg cgggcgggtg
    27661 cgtagcgtgt tcacccaaga ggtgaaggcc atcgcagccg cgagaccccg catcgatgcc
    27721 gccggctcaa aattgacaag cggtcaactg ccgcttctat tgatccggcc tcatgatttg
    27781 gcctcatgag gttttcaccg ttcgccctga gcttgtcgaa gggcttcgac aagctcagcc
    27841 cgaacggaat cttcaaaggg ccggatcaac aggttcaata acccgcatcg atgcgcgcca
    27901 gcccgcccct ggcgcccggg cggcgtcacc cgaccggcac aaagcccagc cgcggcaccg
    27961 cccgccacgt tccgcccaca agccccggca ccacccagac cgccggccat ccaggcactg
    28021 tggcgtgttg cagacgcttc ggcggctaaa cccgggcgat cacggatgga tcaactgcag
    28081 cttgttacat gtgcggcaga ataggtacat tccctagtgt gcagagccgc cccaccgatg
    28141 cggcccatgc ccacagaaca ccgatcgagg aggaaccccc atgtatccga ctgccccctg
    28201 gagccaacgc ctgcaccgct cggtgcgcca gcttgcctcc cccctgtggc gacaggccgc
    28261 cagcgccacg ctggcgctga gcgccgcgct gctgtccagc ccccggcggc cacccaaact
    28321 gccccacttg tggccaccca aactgctcca cc
//
LOCUS       DAOQTZ010000022        27862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2009_length_27862_cov_13.354012, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000022 DAOQTZ010000000
VERSION     DAOQTZ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 27862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27862
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2009_length_27862_cov_13.354012"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..814
                     /gene="can"
                     /locus_tag="PKN51_04295"
     CDS             140..814
                     /gene="can"
                     /locus_tag="PKN51_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNT84352.1"
                     /translation="MPSSLQHLIDANHAWATRMEREHPGFFRSLAQQQSPKYLWIGCS
                     DSRVPANQVTGLAPGEVFVHRNVANVVVNSDLNALSVIQFAVDVLKVEHIMVVGHYGC
                     GGVLAVLREDRVGLADNWLRHVHDVKMRHRGRLLHLCTHDQEDVLCEMNVIEQVGHVA
                     QTTVLQDAWARGQQVSVHGWCYGLKDGLVKDLGVTMSGAGEVVDVYRQAIKRYPRAAA
                     DPGPEE"
     gene            819..2717
                     /gene="aceK"
                     /locus_tag="PKN51_04300"
     CDS             819..2717
                     /gene="aceK"
                     /locus_tag="PKN51_04300"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNT84353.1"
                     /translation="MSLPAIDSGLARDIAQAMIDGFNRHYRLFRTESARAKHRFETCD
                     WHGQQRAQRERIEFYDLRVRECQKRLEREFRAHQQPMAVWHQVKLLYIGLLVTHPQPE
                     LAETFFNSVTTRILHRSYFHNDFIFVRPAISTEYMETTDPKAPPTFRAFYPGDAPLAH
                     TVRAMLESCELRCRWDDMERDAGLVARAMQRELGTARPRSNFQLQVLSSLFYRNKGAY
                     LVGKLINGFQEIPFALPILHRQPGTLHVDAALFGEDDLAILFSFSRAYFMVDMEIPSA
                     YVQFLRSLMPRKPRAELYNALGLAKQGKNLFYRDLLWHLRHSSDQFRIAPGIKGMVML
                     VFDLPSFPYVFKLIRDRFAAPKETTREQVRAKYQLVKRHDRVGRMADTMEFSLLAFPR
                     QRFTDELVAELEQHAASLLEISDRDGDGETEVIIAHAYIERRMIPLNLYLQEALDAGA
                     GDAAASAQLEHAVIEYGNAIKDLVAANIFPGDMLWKNFGVTRHGKVVFYDYDEIEYVT
                     DCQFRRVPPPRHEEDELSGEVWYQVARNDVFPETFAPFLLGHPRVREVFLRHHADLLT
                     PEFWQQNKERIQRGEVIDFFPYGTHRRFDCNGGVAGAPELADPLAAHHETPSGPPDAL
                     APLAPVPE"
     gene            2769..3950
                     /locus_tag="PKN51_04305"
     CDS             2769..3950
                     /locus_tag="PKN51_04305"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNT84354.1"
                     /translation="MSDPIVIVSAARTPIGSFQGDFSSLAAHDLGGAAIRAAIERAGI
                     QPELVTEVLFGNCLLAGQGQAPARQAAFKGRLPKSAGAVTLSKMCGSGMRAAMFAHDM
                     LVAGSHDVLVAGGMESMTNAPYLLQKGRGGYRMGHDKVYDHMMLDGLEDAYEPGRSMG
                     TFGEDCAARYHFTREQQDAFATASVRRAQAAIESGAFQAEITPVTVKERKGERVVDTD
                     EGPGRIKLDNIPKLKPAFKKDGTVTAASSSSINDGAAAMVLMRESTARRLGCRPLARI
                     ASHATHAQEPEWFSTAPIGATEKALAKAGWQVGDVDLWEINEAFAVVPMALMHDLKVP
                     HEKVNVNGGACALGHPIGASGARIMVTLIHALKARGLKKGLATLCIGGGEATAVALEM
                     L"
     gene            4024..4662
                     /locus_tag="PKN51_04310"
     CDS             4024..4662
                     /locus_tag="PKN51_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNT84355.1"
                     /translation="MIDSLDALRALYPEASARSLAKQLDRLDEAMCRFVAHAPLCVVA
                     SVGVGGALADASPRGGLPGFVRVPDARTLWIPDATGNNRLDTLENLLADPRIGLLFLV
                     PGVDEVLRVNGTARLRDEDVFTQRFAAEGVKAPRLVIEVAVREAYLHCPKALLRARLW
                     SPEVRAAPGTFPSMNAMLRAQLGSAVITETDEQARERYRAQLADEGLAHHVR"
     gene            4675..5346
                     /locus_tag="PKN51_04315"
     CDS             4675..5346
                     /locus_tag="PKN51_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT84356.1"
                     /translation="MPTTLILGASRGIGLELARQSLAAGERVIATARSDAGLQTLRAL
                     GAQALRLDVAEPASVSGLAWQLDGEQIDSAWYVAGVMCARADARQPPTAPAFDRVMHT
                     NVLGAMQVIPQVAPLVEAAGGRFGFISSTMGRIGDVEASNAWLYRVSKAALNMVVASA
                     RHSYPRAVLAAFHPGWVQTDMGGAQAPVTVTDSVAGLRRTLAALTLADSGAFLRYDGQ
                     RLASW"
     gene            complement(5419..5988)
                     /locus_tag="PKN51_04320"
     CDS             complement(5419..5988)
                     /locus_tag="PKN51_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNT84357.1"
                     /translation="MTPAPDPVDAAFAAQPREGFLPLDQRPLAALDRPLPIGWGQTNS
                     QPSTVANMLRLLQVAPGMRVLDVGAGSGWTTALLAHLVGPQGQVHGVERVPELVRFGA
                     DNLAATGQPWARIVAAEPGQLGLPALAPFDRILVSAEADRLPQALVDQLADHGLMVIP
                     VNGSMHSVRRRGDELELGTHGEYRFVPLR"
     gene            complement(6017..6283)
                     /gene="infA"
                     /locus_tag="PKN51_04325"
     CDS             complement(6017..6283)
                     /gene="infA"
                     /locus_tag="PKN51_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNT84358.1"
                     /translation="MAKEELIEMQGIVNEVLPDTRFRVTLDNGHELVAYSAGKMKKNH
                     IRILAGDRVSLELSPYDLTKGRISFRHLERRGPMPTGGGGQRRR"
     gene            6769..7110
                     /locus_tag="PKN51_04330"
     CDS             6769..7110
                     /locus_tag="PKN51_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84359.1"
                     /translation="MTPRRLSRGAAVVLMTTALAWNLPAAAQIAPASDGGATAVTSKP
                     EAARASVPPAAAAGQPGPGERARGGTFGGGRAASAAADPVAPAPTPPASGPATGADVP
                     PPPAIHAPGKS"
     gene            complement(7197..7922)
                     /locus_tag="PKN51_04335"
     CDS             complement(7197..7922)
                     /locus_tag="PKN51_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84360.1"
                     /translation="MTVPADPAEEPSLRPPEAEPQRLGSWLLAAVLVLVIVGGAWRAY
                     QWLVTDVERRRAVAEAPPPASAAPAVVAASAAPAPLGRTVTAPQAGVPPEAAAPAAVG
                     QAIHRCRIDGQLTYTNLPCAPGTEDLPAPALGADPNGVVGLAGDSVPSVQPAELSLEG
                     ASRSQRDAACAYLAAEIARLEFEFQQPLPPPVLDHLSTRLSGLRARQRAAECGAPAVP
                     AADQASATRRPASPRAADARRAP"
     gene            complement(8085..8351)
                     /gene="minE"
                     /locus_tag="PKN51_04340"
     CDS             complement(8085..8351)
                     /gene="minE"
                     /locus_tag="PKN51_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNT84361.1"
                     /translation="MSFLSFFLGEKKKTASVAKERLQIILAHERAGRSAAEPDYLPDL
                     QRDLISVISKYVRIDPKDIKVHLERQDDLEVLEVKIELPEKKPD"
     gene            complement(8356..9171)
                     /gene="minD"
                     /locus_tag="PKN51_04345"
     CDS             complement(8356..9171)
                     /gene="minD"
                     /locus_tag="PKN51_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNT84362.1"
                     /translation="MARIVVVTSGKGGVGKTTTSAAFASGLALKGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEANLNQALIKDKQCDNLFVLAASQTRDKDALTKDGV
                     EKVLKDLAAMDFDYIVCDSPAGIETGALLAMHFADEALIVTNPEVSSVRDSDRILGML
                     GSKTQRAIEGKEPVKEHLLITRYNPNRVADGQMLSLDDIQEILRVKLIGVIPESEAVL
                     QASNQGLPAVHLKGSDIAGAYMDVIERFLGAEVPMRYVEAEKPGFFKRLFGGR"
     gene            complement(9216..9974)
                     /gene="minC"
                     /locus_tag="PKN51_04350"
     CDS             complement(9216..9974)
                     /gene="minC"
                     /locus_tag="PKN51_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNT84363.1"
                     /translation="MSVAVAGRSPTTFDIKSASLPLLTLRLKSPDLARLAEELLAQYG
                     DRPDFFEDDAVVLDLSTLPAEQAGAEIDFQQLGELLQARRLRLLAVRGGSAAQHEAAR
                     AIGLLQAPDLRVAQAAAPDAAPARRADSAAVEPPPPGALVIERPLRSGQQVYARGRDL
                     VLLAMVNAGAEVIADGHIHVYAPLRGRAIAGARGWAEARVFALEMRPDLVSIAGVYRT
                     GDEPLPPSVWGQRASVRLASGEVGDKLIFEPVAG"
     gene            10694..11635
                     /locus_tag="PKN51_04355"
                     /pseudo
     CDS             10694..11635
                     /locus_tag="PKN51_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(12149..12358)
                     /locus_tag="PKN51_04360"
     CDS             complement(12149..12358)
                     /locus_tag="PKN51_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HNT84364.1"
                     /translation="MFGFNEEEIAWFGLTFGVSAFILYMLFIIGQLAWESKAGKFGTF
                     VLFLALAFGMVGFIAKGVIQWIIEK"
     gene            complement(12358..13221)
                     /locus_tag="PKN51_04365"
     CDS             complement(12358..13221)
                     /locus_tag="PKN51_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HNT84365.1"
                     /translation="MNHTHLPPPLPPRPARGTLTLPAVRTVALDAPLHWLALGWRDLW
                     RVGPLSLLYGALLAGFGGLLLALAADQFWLLAGAFSGFLVIAPVLATGWYALSRALER
                     GEPANAATVRKTWIRWQYSRYAVHGGYWSLVRFGLLLALAGSGWVLTSAALITALAPQ
                     PVHTPLDFLRHVVLAPGGHLFELWLMLGALMAAPLFASSVVAMPLLLDRRADVLQAVL
                     TSWQAVLNNPAPLALWAALIMLLTLLGMATVLLGLVLALPWLGHASWHAYRALVDADS
                     LPERLPPEGGA"
     gene            complement(13382..13921)
                     /locus_tag="PKN51_04370"
     CDS             complement(13382..13921)
                     /locus_tag="PKN51_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNT84366.1"
                     /translation="MNLHRKHLLLASLLAATGTLALAQAPAAGDAPARPGWSERRAER
                     MDPARMQQRMAERHAQRMDKLKAELKLNPTQEGAWTQFSTAMQPPAFEPRGPGARADF
                     AKLSTPERIDLMQKRADERHARMKQRGDATKAFYAQLTPEQQQSFDRLAARGRHGAGW
                     HAGHREHRGWGPGMGPGPR"
     gene            complement(14089..14511)
                     /gene="cueR"
                     /locus_tag="PKN51_04375"
     CDS             complement(14089..14511)
                     /gene="cueR"
                     /locus_tag="PKN51_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HNT84367.1"
                     /translation="MSATPASGPIGIGEAARASGVSAKMVRHYESLGLLGQVARTDSG
                     YRQYGAADVHTLRFIRRARDLGFSMAEIAELVSLWHDRSRASADVKRIAQRHLAELEQ
                     RIAALQDMRRTLDQLVCHCHGDARPDCPILDDLAGTSG"
     gene            complement(14508..14708)
                     /locus_tag="PKN51_04380"
     CDS             complement(14508..14708)
                     /locus_tag="PKN51_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNT84368.1"
                     /translation="MNQTFTVTGMTCGHCEASVKRALLQLDPGAQVQIDRSQNRVEVS
                     GSQQSREALAAAIREEGYDVAA"
     gene            14843..17122
                     /locus_tag="PKN51_04385"
     CDS             14843..17122
                     /locus_tag="PKN51_04385"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNT84369.1"
                     /translation="MNTTTASSTVDLGVGGMTCASCVARVEKALQKVPGVDSAAVNLA
                     TESARVHFVGDAGELGPLLRRAVRDAGYEPRAAEAALAEREGGPWAGFLPVGIGLLLS
                     APLVLPMLGMPFGWHWMPPPWVQFLLATPVQFWLGARFYRAAWHALKARSGNMELLVS
                     IGTTAAWLLSMWLWLGKSHPPGHEPHLYFEGAAVVITLVRLGKWLEERAKRQTTAAIR
                     ALHALRPEVAHLIDWDGSEKDIPVDELRAGDRLVVRPGERVPADGRLAEGATQVDESM
                     LTGEPLPVAKAVGDALTGGSINGEGRVVLRVTAAGAESVLAHIIRLVQDAQAVKAPVQ
                     RLVDQVAAVFVPVVLLIALATLLGWWLLGGTGLEVAIMRAVAVLVIACPCALGLATPT
                     AIMAGTGVAAKFGILIKDAEALEIAHRVRTVAFDKTGTLTVGQPRLLALLPAPGVDEA
                     AALRLAAGLQSGSEHPLARAVLAQAGARGLWPPAEGTAAELAELRAVPGRGTEGRVGQ
                     QRVRIGSARWMAELGVDLQVFEALAQAQQAQGATVSILATQPAEAPEGTPPRAVALLA
                     FGDEPKPGAAAALAELHARGIRAVMISGDNRGAAEAMARRLGLKPEDGEVLAEVLPGD
                     KAQAVRQLRAGGAVVGMVGDGVNDAPALAAADVGMAMGSGTDVAMHAAGITLMRGDVG
                     LVPAALDISQRTVAKIRQNLFWASFYNAAGIPLAALGYLSPVIAGAAMALSSVSVMSN
                     ALLLRRWRPASQGSVGTPG"
     gene            17263..17847
                     /locus_tag="PKN51_04390"
     CDS             17263..17847
                     /locus_tag="PKN51_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNT84370.1"
                     /translation="MKHRSLEIIRDEHSSLAAMLQSMRLMVDRGPQDDPRNFFDVLRA
                     MLFYIDEFPERLHHPKETELLFPRVAAAAPEVGMAVARLDHDHEYTEKAVRDIQHLLL
                     GWELLGESRRQRFIDAFHRYVNLYLEHMRLEEQEVLPAAEKHLTEADWRELDAEFEKN
                     CDPLTGKYKPDPVYEKLFTRIVRDAPAPIGLGGS"
     gene            17996..19108
                     /locus_tag="PKN51_04395"
     CDS             17996..19108
                     /locus_tag="PKN51_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HNT84371.1"
                     /translation="MKSRAAVAFAAGQPLEIVEIDVAPPRKGEVLVRITHTGVCHTDA
                     FTLSGDDPEGVFPAVLGHEGAGIVVECGEGVTSVKPGDHVIPLYTAECGECLFCKSGK
                     TNLCVAVRATQGKGVMPDGTTRFSYNGQPVYHYMGCSTFSEYTVVAEVSLAKIDPAAN
                     PEQVCLLGCGVTTGLGAVKNTAKVQPGDSVAVFGLGGIGLAVVQGARLAGAGRIIAID
                     TNPAKFDLARTFGATDCVNPKDFDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALEC
                     AHRGWGQSVIIGVAGAGQEISTRPFQLVTGRRWLGTAFGGVKGRSELPGMVQDAMAGR
                     IQLAPFVTHTMGLTDINQAFDLMHEGKSIRSVLRHG"
     gene            19185..20015
                     /gene="fghA"
                     /locus_tag="PKN51_04400"
     CDS             19185..20015
                     /gene="fghA"
                     /locus_tag="PKN51_04400"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HNT84372.1"
                     /translation="MERIEQHASFGGRQEVWRHASASTGTPMNFGIYLPPQALAGQRC
                     PVLYWLSGLTCSEQNFITKAGVQPHAAAHGLIVVAPDTSPRGPGVADDAAYDLGQGAG
                     FYLNATQAPWAAHYRMQDYVADELPALIERHFPASDARGIFGHSMGGHGALVTALRHP
                     GRYRSVSALAPIAAPTQVPWGHKALAAYLGDDPAAWAGWDAVALIATARERLPLLIDQ
                     GEADEFLPQLRPDLLRAACEAAGHPLTLRLQPGYDHSYYFIASFIGEHVAHHAGALRG
                     "
     gene            20129..20359
                     /locus_tag="PKN51_04405"
     CDS             20129..20359
                     /locus_tag="PKN51_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HNT84373.1"
                     /translation="MADTWLPTLITDTPQQGFELAITLSRKGVRYTQPDPEVLHKLRP
                     VYANDAHELTAASQVIALNFQTVAAANNYWRK"
     gene            complement(20435..21562)
                     /locus_tag="PKN51_04410"
     CDS             complement(20435..21562)
                     /locus_tag="PKN51_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNT84374.1"
                     /translation="MAQTLFTATRVGAIDVASRLVMAPLTRNRAPGAIPNALMAEYYA
                     QRADPLSGAGLIVSEGTAISPQGQGYADVPGLYGSEQIDGWKQVTQAVHARGGRIVCQ
                     LWHVGRVSHHVLQPGHADPVAPSALRANAKTYVFDAQGHGHFAPTSMPRALHRHEIPG
                     IVHDYAAAARHAVQSAGFDGVEIHGANGYLLDQFLKSGANHRTDDYGGRIAHRARLML
                     EVARAVCDAVGADRVGIRLSPVTPANDIVDADPQPLFEHLMRQLAPLGLAYIHVIEGA
                     TGGPREVEGRPFDYAAMKAAYRAAGGQGAWIVNNGYDPQSAEAALASGYADLVAFGKA
                     FISMPDLAARIRRGGPYQGLERATMYGGGAHGYTDYPALAN"
     gene            21678..22895
                     /locus_tag="PKN51_04415"
     CDS             21678..22895
                     /locus_tag="PKN51_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNT84375.1"
                     /translation="MSPPAPPLSRLLWLALALSLGAAVSLGITRFAYALLLPVMREDL
                     AWSYTLAGAMNTANALGYLLGAIATPWLMRRWSASTVLLTGSALASVFMAASGFFTDA
                     VPLLLQRLLAGAASALVFVSGGLMAARLGAQSPQRAGLLLGLYYGGTGWGIAASALGV
                     PLVLQAAQGVAHGWAWAWWGLAALCLAATAVLWWPANALRKQELMAHGASHPQADSPQ
                     NLPWRAMALALAGYTLFGVGYIGYMTFVIALLREQGVSAGAVTLFYTLLGLAVVGSAR
                     LWAGLLDRAQGGGALALLNALLGVATVLPALTGAWPVVLASGLLFGAVFLSLVASTTA
                     FVRHNLPPAQWAAGISAFTILFAFGQIVGPTVVGWIADGPGGLAQGLRYSAGALWLGA
                     LLAWRQRPLARAA"
     gene            complement(22906..23511)
                     /gene="gstA"
                     /locus_tag="PKN51_04420"
     CDS             complement(22906..23511)
                     /gene="gstA"
                     /locus_tag="PKN51_04420"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HNT84376.1"
                     /translation="MKLYYSAGACSLAAHIALEEAGLKYEAISAPTKTKQLPDGSDYR
                     QVNPLGYVPYLVLDDGQALRECAIICQYVADQAPAKALIPPCGSMARYQLQMWMHFTA
                     TELHKGFSPLFNPAMPAEAKQVALARLQERLKFVDGELAGKAFVMGSDYSVADAYLFT
                     VTHWAKPMAIDLAPYPNLLAWRERVAARPAVQAAMKAEGLI"
     gene            complement(23614..23787)
                     /locus_tag="PKN51_04425"
     CDS             complement(23614..23787)
                     /locus_tag="PKN51_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteinase inhibitor"
                     /protein_id="HNT84377.1"
                     /translation="MPATGPQGRCRLFGRPEGPAVCASLRPSPEMCGPPDDGGARALR
                     CLSTLERATGRGG"
     gene            complement(24204..25439)
                     /locus_tag="PKN51_04430"
     CDS             complement(24204..25439)
                     /locus_tag="PKN51_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84378.1"
                     /translation="MVLLNNGGDALTRNANGSFTFATPIASGATYSVTVGTQPVGQTC
                     SVSNGAGTVVASNVTNVTVNCTTNTYTIGGSVSGLGAGNSVVLLNNGGDALTRNANGS
                     FTFATPLANGAAYAVTVGTQPVGQTCSVSNGTGTVASANVTNVAVNCVNVPTYTIGGS
                     VSGLVTGKTVGLLNNGGDALTVNANGSFTFATALTSGSAYAVTVGTQPVGQVCTVSNG
                     TGTVASANVTNVAVSCVNMISGPTSTGTGTATGTLTGGGAACGFAAGSGFVGTPAPPP
                     AGVSFPHGFFGFTATSCPAANGAVTVTITYPTAIPPGAQYYKWGKEPGNTTDHYYTIP
                     ATISGNQVSFTITDGQLGDNDLAANGTITDPGAIGVTAAAAGVAPIPTTSEYVLMALA
                     MLMLLAAASPLRARRGTRR"
     assembly_gap    25443..25542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25543..>27862)
                     /locus_tag="PKN51_04435"
     CDS             complement(<25543..>27862)
                     /locus_tag="PKN51_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT84379.1"
                     /translation="ALIDLTRAAGSGQLRNAASRQALPAYVQDFAERHVAPDQTIFVV
                     IKGKVSDTLTAFIRSRGGTELSAFPQYDTITARVPVSELSAIANRPDVRTVGPREEAT
                     TNRLELPPEVLREKLKNLPQPGAATNAGSATWEGVAAHKANAAHSAGRTGVGTMVCVL
                     SDGVNSLAARQATGDLPATVTVLPGQAGSGDEGTAMLEIVHDMAPGASLGYATAFTSV
                     ASFATNIVNLQAAGCNIIVDDVTYFNEGAFQDGPIAQAVNQVTAAGALYFSSAANSGN
                     KNDNQSGTFEGDFVASGNPIPSAIQSIYGAASIQLHAFGSNNYTTLTAQTRYVSLKWS
                     DPLGGSSNDYDLIVTNAAGTVVLGQSTGNQTGTQDPYELASSGTPYPIGSRIYIVRYS
                     GSARALRLDTHRGRITPADSTAGSTFGHNAAASGISVAAVSLTTATGPGGTFTGGAAN
                     PVETYSSDGPRKIFYHPNGTAITPGNLLFATNGGTTLPKVDMAAGDCGTTTTPGFTTF
                     CGTSAAAPTAAAIAALIKGANPGWTNAQIVAAMNASALDIEAAGWDRDSGVGIVMVPA
                     SLSSFTIGGNVSGLGAGKSVVLLNNGGDALTRNANGSFTFATPLANGAAYAVTVGTQP
                     VGQTCTVSNGTGTVASANVTNVTVNCTSNTYTIGGSVSGLGAGNSVVLLNNGGDALTR
                     NANGSFTFATPIASGATYSVTVGTQPVGQTCTVSNGSGTVGSANVTNVAVNCVNAPPV
                     TYTIGGSVSGLGAGKSVVLLNNGGDALTRNANG"
BASE COUNT         4044 a         9338 c        10057 g         4323 t
ORIGIN      
        1 ccaagctgta cgagatcggc gccggcacca gcgagatccg ccgcatgctg atcgggcgcg
       61 agctgttcgc cgaaaccctg tgaccccagg tcacggcgcc cgccgcaggc gggagccgtg
      121 accgcaccga ccgcgcgcca tgccctcgtc cctgcagcac ctgatcgacg ccaaccacgc
      181 ctgggccacc cgcatggagc gcgagcaccc gggcttcttc cgcagcctgg cgcagcagca
      241 gtcgcccaag tacctgtgga tcggctgctc cgacagccgc gtgccggcca accaggtcac
      301 cggcctggcg ccgggcgagg tgttcgtgca ccgcaacgtg gccaacgtgg tggtcaactc
      361 cgacctcaac gccctctcgg tgatccagtt cgcggtcgac gtgctgaagg tcgagcacat
      421 catggtggtc ggccactacg gctgcggcgg cgtgctggcg gtgctgcgcg aagaccgcgt
      481 gggcctggcc gacaactggc tgcgccacgt gcacgacgtc aagatgcgcc accgcggccg
      541 cctgctgcac ttgtgcacgc acgatcagga agacgtgctg tgcgagatga acgtgatcga
      601 gcaggtcggc cacgtggccc agaccaccgt gctgcaggac gcctgggcgc gcggccagca
      661 ggtgtcggtg cacggctggt gctacggcct gaaagacggg ctggtgaagg atctgggcgt
      721 caccatgtcg ggtgccggcg aagtggtgga cgtctaccgc caggccatca agcgctaccc
      781 gcgcgccgcc gccgaccccg gccccgagga gtgaggtggt gagcctgccg gccatcgatt
      841 cgggcctggc gcgcgacatc gcgcaggcga tgatcgacgg cttcaaccgc cactaccggc
      901 tgtttcgcac cgaatcggcg cgcgccaagc accgcttcga gacctgcgac tggcacggcc
      961 agcagcgcgc ccagcgcgag cgcatcgagt tctatgacct gcgggtgcgc gaatgccaga
     1021 agcggctgga gcgcgagttc cgcgcccatc agcagcccat ggccgtgtgg catcaggtca
     1081 agctgctgta catcggcctg ctggtgacgc acccgcagcc cgaactggcc gagaccttct
     1141 tcaactcggt caccacgcgc atcctgcacc ggagctattt ccacaacgac ttcatcttcg
     1201 tgcgcccggc catcagcacc gagtacatgg agaccaccga ccccaaggcg ccgcccacct
     1261 tccgcgcctt ctaccccggc gacgccccgc tggcgcacac ggtgcgcgcc atgctcgaat
     1321 cctgcgagct gcgctgccgt tgggacgaca tggaacgcga cgccggcctg gtggcgcgcg
     1381 ccatgcagcg cgagctgggc accgccaggc cgcgctccaa cttccagctg caggtgctga
     1441 gcagcctgtt ctatcgcaac aagggggcct acctggtcgg caagctgatc aacggctttc
     1501 aggaaatccc gttcgccctg cccatcctgc accgccagcc cggcacgctg cacgtcgacg
     1561 ccgcgctgtt cggcgaggac gacctggcca tcctgttcag cttctcgcgc gcctacttca
     1621 tggtggacat ggagattccc tcggcctacg tgcagttcct gcgcagcctg atgccgcgca
     1681 agccgcgcgc cgagctgtac aacgcgctcg gtctggccaa gcagggcaag aacctgttct
     1741 accgcgacct gctgtggcat ctgcggcact cgagcgacca gttccgcatc gcgcccggca
     1801 tcaagggcat ggtgatgctg gtgttcgacc tgcccagctt tccctacgtg ttcaagctca
     1861 ttcgcgaccg ctttgccgca cccaaggaaa ccacgcgcga gcaggtgcgc gccaagtacc
     1921 agctggtcaa gcgccacgac cgcgtgggac gcatggccga caccatggag ttctcgctgc
     1981 tggcctttcc gcgccagcgt ttcaccgacg agctggtggc cgagctggag cagcatgccg
     2041 ccagcctgct cgaaatctcc gaccgcgacg gcgacggcga gaccgaagtc atcatcgcgc
     2101 acgcctacat cgagcggcgc atgatcccgc tcaacctgta cctgcaggag gcgctggacg
     2161 ccggcgccgg cgatgccgcc gccagcgcgc aactggagca cgccgtcatc gagtacggca
     2221 acgccatcaa ggacctggtg gcggccaaca tcttccccgg cgacatgctg tggaagaact
     2281 tcggcgtcac ccgccatggc aaggtggtgt tctacgacta cgacgagatc gagtacgtca
     2341 ccgactgcca gttccgccgc gtgccgccgc cgcgccacga ggaagacgag ctgtccggcg
     2401 aggtctggta ccaggtggcc cgcaacgacg tgtttcccga aaccttcgcg ccctttctgc
     2461 tcggccaccc gcgcgtgcgc gaggtcttcc tgcgccacca cgccgacctg ctgacgcccg
     2521 agttctggca gcagaacaag gaacgcatcc agcgcggcga ggtgatcgac ttcttcccct
     2581 acggcacgca ccggcgcttc gactgcaacg gcggcgtcgc cggcgcgccc gagctggccg
     2641 acccgctggc ggcgcaccac gaaaccccgt ccggtccgcc cgatgccctg gcacccctgg
     2701 cgccggtgcc cgaatgaacg cctgaactgc gccctgccat cccgtttctg cttgaaagga
     2761 aaacagccat gtccgacccc atcgtcatcg tttccgccgc ccgcacgccc atcggcagtt
     2821 ttcagggcga tttctcgtcg ctggccgcgc acgacctggg cggcgccgcc atccgggccg
     2881 ccatcgagcg tgcgggcatc cagccggaac tcgtcaccga ggtgctgttc ggcaactgcc
     2941 tgctggccgg ccagggtcag gcgccggcgc gccaggccgc gttcaagggc aggctgccca
     3001 agagcgcggg ggcggtgacg ctgtccaaga tgtgcggctc gggcatgcgt gcggccatgt
     3061 ttgcgcacga catgctggtg gccggcagtc acgatgtgct ggtggccggc ggcatggaga
     3121 gcatgaccaa tgccccgtat ctgctgcaaa aagggcgcgg gggctatcgc atggggcacg
     3181 acaaggtgta cgaccacatg atgctcgacg gcctggagga cgcctacgag cccggccgca
     3241 gcatgggcac cttcggcgag gactgcgccg ccaggtacca cttcacgcgc gagcagcagg
     3301 acgcctttgc caccgccagc gtgcggcgcg cgcaggccgc catcgagagc ggtgccttcc
     3361 aagccgagat cacgccggtc acggtcaagg agcgcaaggg cgagcgcgtg gtcgacaccg
     3421 acgaagggcc gggccgcatc aagctcgaca acatccccaa gctcaagccc gccttcaaga
     3481 aagacggcac ggtgacggcg gccagcagct ccagcatcaa cgacggtgct gccgccatgg
     3541 tgctgatgcg cgagtccacc gccaggaggc tcggctgcag gccgctggcg cgcattgcca
     3601 gccacgccac gcacgcacag gagcccgagt ggttttccac cgcgccgatc ggcgccaccg
     3661 aaaaggcgct ggccaaggcc ggctggcagg tgggcgacgt cgatctgtgg gaaatcaacg
     3721 aggcctttgc cgtggtgccg atggcgctga tgcacgatct gaaggtgccg cacgagaagg
     3781 tcaacgtcaa cggcggcgcc tgcgcgctgg gccacccgat cggcgccagc ggcgcacgca
     3841 tcatggtgac gctgatccac gccctgaagg cgcgcggcct gaagaagggc ctggccacgc
     3901 tgtgcatcgg cggtggcgag gcgaccgccg tggcgctcga aatgctatga atagcatagc
     3961 tgttggcgcc cgacggacgg gcgccaatgg ccttttgatc tgtattccga tcggagtcgc
     4021 ccgatgatcg attcgctcga cgcgctgcgt gcgctctacc cggaggccag cgcccggtca
     4081 ctggccaagc agctcgaccg gctggacgag gccatgtgcc gcttcgtcgc ccatgcgccg
     4141 ctgtgcgtgg tggccagcgt gggggtcggc ggcgcactgg ccgacgcgtc gccacgcggt
     4201 ggtctgccag gcttcgtgcg agtgccggat gcgcgcacgc tctggatccc cgacgccacc
     4261 ggcaacaacc gcctggacac gctggagaac ctgctggccg acccgcgcat cggcctgctg
     4321 tttctggtgc cgggggtgga cgaagtgctg cgcgtcaacg gcacggcgcg cctgcgcgac
     4381 gaagacgtct tcacgcagcg ctttgccgcc gagggcgtga aggcgccgcg gctggtgatc
     4441 gaagtcgccg tgcgcgaggc ctacctgcac tgccccaagg cgctgctgcg ggcgcgcctg
     4501 tggtcgcctg aggtgcgcgc ggcgcccggc accttccctt ccatgaacgc catgctgcgc
     4561 gcccagctgg gcagcgcggt gatcaccgaa accgacgaac aggcgcgcga acgctaccgc
     4621 gcccagctgg ccgacgaagg cctggcgcac cacgtgcgct gaccgccgaa caccatgccc
     4681 accaccctga ttcttggcgc ctcgcgcggc atcggcctgg agctggcacg ccagtcgctg
     4741 gccgccggcg agcgcgtgat cgccaccgcc cgctccgacg ccggcctgca aaccttgcgc
     4801 gcgctggggg cacaggcact gcggctggac gtggcggagc cggccagcgt cagcggcctg
     4861 gcctggcagc tcgacggcga gcagatcgac agcgcctggt acgtggccgg cgtgatgtgc
     4921 gcgcgcgccg atgcgcgcca gccgcccacg gcaccggcgt tcgaccgcgt gatgcacacc
     4981 aacgtgctgg gcgcgatgca ggtcatcccg caggtggcgc cgctggtgga agcggcgggc
     5041 gggcgcttcg gtttcatctc cagcaccatg ggccgcatcg gcgacgtcga ggccagcaac
     5101 gcctggctgt accgcgtcag caaggcggcg ctgaacatgg tggtggcctc ggcgcgccac
     5161 agctacccgc gggcggtgct ggcggcgttt catcccggct gggtgcagac cgacatgggc
     5221 ggcgcccagg caccggtgac ggtgaccgac agcgtggccg gtctgcgccg cacgctggcg
     5281 gcgctcacgc tggccgacag cggcgccttc ctgcgctacg acgggcagcg gctggcgtcg
     5341 tggtgaaacg gcatgaccca tgagccgcgt ggcgatcgac gcgccgaagg gcgccattcg
     5401 tcatgcagcc tgccgccgtc accgcagcgg cacgaagcgg tactcgccgt gcgtgccgag
     5461 ttcaagctcg tcgccgcgcc gccgaacgct gtgcatgctg ccgttcaccg ggatcaccat
     5521 caggccgtgg tcggccagct gatcgaccag cgcctgcggc aggcggtcgg cctcggccga
     5581 caccaggatg cgatcgaacg gggccagcgc cggcaggccg agctggcccg gctcggcggc
     5641 cacgatgcgg gcccagggct ggccggtggc ggccaggttg tcggcgccaa agcgcaccag
     5701 ttcgggcacc cgctccaccc catgcacctg gccctggggc ccgaccaggt gcgccagcag
     5761 ggccgtggtc cagccggaac cggcacccac atccaacacc cgcatgccgg gtgcgacctg
     5821 cagcagccgc agcatgttgg ccaccgtgct gggctgggaa ttggtttggc cccagccgat
     5881 cggcagcggg cgatccagcg cggccagggg gcgctggtcg agcggcagaa aaccctcgcg
     5941 cggctgggcc gcgaaggcgg cgtccaccgg gtcgggcgcg ggcgtcatgg ccccggcgtc
     6001 gatggcggct gccggctcag cggcggcgct gcccaccgcc gccggtgggc atggggccgc
     6061 ggcgctccag gtgacggaag ctgatgcggc ccttggtgag gtcgtagggc gacagctcca
     6121 gcgacacccg gtcacccgcc aggatgcgga tgtggttctt cttcatcttg ccggcggaat
     6181 aggccaccag ctcgtggccg ttgtccagcg tgacgcgaaa gcgcgtgtcc ggcagcactt
     6241 cgttgacgat gccttgcatc tcgatcagtt cttccttggc catgcgttgc tcctaaaaca
     6301 tgatgcggtg tgggcaccgc cgggcatgcc cgatgggcgt gccgcgcggc gcgcagggtt
     6361 caggttcgcc gggcgtcaaa gtggccgtga ggcgcgggct cggacgcggc acctgatcag
     6421 ccgttccgtt gccactgggt gcaggtgcgc tggcaagacc aggatccgaa gatggcgccg
     6481 acaggacccg gcttgggcgc catccacccg gtcgcgcacg tgcaactcct ggggttagcc
     6541 ggcaattgta tccggcgcag ggtttttctg ccgcgctttc tgcggtggca cgccgcgccc
     6601 gggcggccgc agggtggtgc tgcgaccacc ggtggcgccg caccaggtgt cgggcaggtg
     6661 taaagtgagt gtgcattcac acccttacaa ctcgaacaca gaagccatgt ttgtaagcct
     6721 ggatggcgtc agactggcag ggtgttcaag ttcggaaagg ttgtttccat gaccccacgc
     6781 agactctcgc gcggcgccgc cgtcgtgctc atgaccaccg ccctggcctg gaacctgccg
     6841 gcggcggcgc agatcgcgcc ggcctccgac ggcggcgcca ccgccgtcac gtccaagccc
     6901 gaggctgcgc gtgcgagcgt gccgccagcg gcggcggcgg gccagccagg cccgggcgag
     6961 cgcgcacgcg gcggcacctt tggcggcggc cgcgcggcct cggcggccgc cgacccggtg
     7021 gcgccagcgc cgaccccgcc cgcctcaggc ccggccacgg gcgcagacgt accgccgccg
     7081 cccgccatcc acgcgccagg caaatcctga aggcccactc gccggccacc ggcacccgtt
     7141 gttgacgcga atcggggcaa gcgccgatcg ctattcaaaa cgtagcaatc gcgccctcac
     7201 ggggcgcgcc tggcgtccgc cgctcggggg ctggccgggc ggcgcgtggc gctcgcctga
     7261 tcggctgccg gcacggcggg ggcgccgcat tcggcggcgc gctggcgggc gcgcagtccg
     7321 ctcagccggg tcgacagatg gtccagcacc ggcggtggca gcggctgctg gaactcgaac
     7381 tccagccggg cgatttcagc cgccaggtag gcgcaggcgg cgtcgcgctg gctgcggctg
     7441 gcgccttcca ggctcagctc cgccggctgg accgacggca cgctgtcgcc ggccaacccg
     7501 accacgccgt tggggtcggc ccccagcgcg ggcgcgggca ggtcttcggt gccaggcgcg
     7561 cagggcaggt tggtgtaggt cagctgaccg tcgatgcggc agcgatgaat ggcctggccc
     7621 accgcggccg gtgccgcagc ttcgggcggc acgccggcct gcggcgcggt gacggtgcga
     7681 ccgaggggtg ccggtgccgc cgatgccgcc accaccgcgg gcgcggcgga ggctggcggc
     7741 ggcgcctcgg ccacggcacg ccgacgctcg acgtcggtca ccagccactg gtaggcacgc
     7801 caggcgccgc cgacgatcac cagcaccagc acggccgcca gcagccagct gcccaggcgc
     7861 tgcggctcgg cctcgggcgg ccgcagggac ggttcttcgg cgggatcggc aggcacggtc
     7921 atggcacggc aggagcgggc gacggcccgc gcgaccaggg atgatacggg agccggcacg
     7981 gggtcgcgcg ggcgccgcgc gcaggcgttg gcccacccct tgaaagctat tcttttaata
     8041 gctgttggcg ctgattggaa aagcgccaga ggccgaaaag gccttcagtc cggctttttc
     8101 tcgggcagtt cgatcttgac ctccagcacc tccaggtcgt cctggcgttc caggtgcacc
     8161 ttgatgtcct tggggtcgat cctgacgtat ttgctgatga ccgaaatcag gtcgcgctgc
     8221 aggtcgggca ggtaatccgg ctcggcggca ctgcggccgg cgcgctcgtg cgccaggatg
     8281 atctgcagac gctccttggc gacgctggcg gtcttcttct tctcgcccag aaagaatgac
     8341 aggaaggaca tggcttcagc gccccccgaa caggcgcttg aagaagccag gtttctcggc
     8401 ctcgacgtag cgcatcggca cctcggcgcc cagaaagcgc tcgatcacgt ccatgtaggc
     8461 accggcaatg tcgctgccct tcaggtgcac cgccggcagg ccctggttgg aggcctgcag
     8521 cacggcttcg ctctcgggaa tgacgccgat cagcttgacg cgcaggatct cctggatgtc
     8581 gtccaggctc agcatctggc cgtcggcgac gcggttcggg ttgtagcgcg tgatcagcag
     8641 gtgctccttg accggctcct tgccctcgat ggcacgctgc gtcttgctgc ccagcatgcc
     8701 caggatgcgg tcggagtcgc gcacgctgga gacctcgggg ttggtgacga tcagcgcctc
     8761 gtcggcaaag tgcatggcca gcagcgcgcc ggtctcgatg ccggcgggcg agtcgcacac
     8821 gatgtagtcg aagtccatcg ccgccaggtc cttgagcacc ttctcgacgc cgtccttggt
     8881 cagcgcgtcc ttgtcgcgcg tctggctggc ggccagcacg aacaggttgt cgcactgctt
     8941 gtccttgatg agggcctggt tcaggttggc ctcgccctgg atcacgttga tgaggtcgta
     9001 caccacgcgg cgctcgcagc ccatgatgag gtcgaggttg cgcaggccga cgtcgaagtc
     9061 gatcaccgcc gtcttgtgcc ccttgagcgc cagcccggac gcgaacgcgg cgctggtggt
     9121 ggtcttgccc acgccacctt tgcccgaagt caccaccacg attcttgcca tgtcgatctc
     9181 gcttgcaaaa tgaaaaccgt cgtcgccgcg cgccttcagc ccgccacggg ttcgaaaatc
     9241 agcttgtcgc cgacctcgcc gctggccagg cgcacgctgg cgcgctggcc ccacacggaa
     9301 ggcggcaggg gttcgtcgcc ggtgcggtag acgccggcga tcgacaccag gtcgggccgc
     9361 atctcgagcg cgaacacgcg cgcctcggcc cagccgcgcg cgccggcgat ggcgcgcccg
     9421 cgcagcggcg catagacgtg gatgtggccg tcggcaatca cctctgcccc cgcgttgacc
     9481 atggccagca gcaccaggtc gcgcccgcgc gcgtacacct gctggcccga gcgcagcggc
     9541 cgctcgatca ccagcgcgcc gggcggcggc ggctcgacgg cggcgctgtc ggcacggcgc
     9601 gccggcgcgg catcgggcgc cgccgcctgc gccacccgca ggtcgggcgc ctgcagcagg
     9661 ccgatggcgc gcgccgcctc atgctgcgcg gcgctgccgc cacgcaccgc cagcaggcgc
     9721 agacggcgcg cctgcagcag ctcgcccagc tgctggaagt cgatctcggc ccccgcctgc
     9781 tcggccggca gggtggacag atcgagcacc acggcgtcgt cctcgaagaa gtcgggccgg
     9841 tcgccgtact gcgccagcag ctcctcggcc agccgcgcca gatcgggcga cttcaggcgc
     9901 agcgtcagca gcggcaggct ggcgctcttg atgtcgaagg tggtggggct gcggccggcg
     9961 acggcaacgg acatggcggg cggtcaaaaa cgttcccgca ggcgggacgc agggaagcgg
    10021 ggcgatctta acagtcgcca gcggcgtgaa cggcgccggg ccgggcatcg acggggcgga
    10081 gttgtgccgg tttgtttttc ttgagtcttt tgaattcgta gtagtcgtag taggtgccct
    10141 gccgttttgt ggacagcacg ggtttcccta gtcctggtgc gcacttgcag cgcctgcgcg
    10201 gctgtgcaca agcggccgcg gccgtggtgc ggccgtgggt acaagcggtc agggtcgttg
    10261 ccgcctgtgg ataaacagat ggttttacaa gaaacatcca cagacttgct ccagtcgatc
    10321 ctgtgtgatg ctggctcatc tttgcacgtc gggccaggaa agcacttggc caaccctctt
    10381 ttccttttgg gatgaccctg gcaggggcat gatcagcatc gtattgccgt ttttccggaa
    10441 acttgcggac tttcgcaggg tgttgccgct caaccgaccg tacttcgccc ggccctgggt
    10501 cgaggtggtg gtggtgatgg atgagaacga gtcggaatcg ggtgtgctcg acctgatccg
    10561 ccaatacccc gacattcgct ggcaggtgag ggtgaacgac cagcctcatg gatggcaacc
    10621 gccctgcaag gcgatcaacg tcggggtgag gcacgcgtcg cacgaggtgg tgctggtctg
    10681 ttcgcccgaa tcgatgttcg tgaccgatgt gccggcacag gcgctgggca cggttcgggc
    10741 ggtgccacgc ggggtggcgc tggggcgtgt gggctttgga cagtacgcgc agctgcatca
    10801 gctgggggcg tcggccgctt tcgacgcggt ggctcccgat gcccgcgatc cgttcaagct
    10861 ctacggttcg atcgcgctgg cgcggcagga actggagctc atcggtggct acgacgagag
    10921 ctttcgcggc tggggtgccg acgacgacaa cgtgcgcgtt cgcctggcca tgaacggtgc
    10981 gcgcctgctg ggttgccccc agatgaagct gctgcacctg gcggacgaac ctcgccgccc
    11041 cagtccggca ttcaatgcag cctcggatct gaggaaatgc tccccggcga cggcgcgtgc
    11101 caaccttggt gtggcatggg ggcgggcgtt ttcccgcctg gcctggccga tcggcagcca
    11161 gcctggcggc cgccccgagg ttgccgcgac agtgcctgtt cccgtggtca gcaatccagc
    11221 ctttccggtg ccgtcccgcc agcgctgccc ggtttgtggt cgcttcaccc agttgcagca
    11281 ggcgctgtcc ttctgccttc gctgtgtgcc gcagccgcac gaccaggtgt cggacgcgcc
    11341 gcgcctgctg tgcgtcatgc aggtccgcaa tgaagcgcgc gttctgccgg gctgcttcga
    11401 gcatctgcgc gggtatgtgg atggtttcat cgtgcttgac gatggctcga ccgacggcac
    11461 gcgggagatt gtcagccgcg aacccgcctt gctggagctg ctcgacaacc ccccggccca
    11521 gccccatgtc tggaacgagc gggacaacaa gcgccggctg ctgcaggccg cgcgtcgtca
    11581 cggcgcgcaa tgggtgctgg tgtgcgatgc cgacgagcgc tacgagatga tgttcgtcaa
    11641 ggggctgcgc cctctcggca aggcgctcga cgcgctgggt tttcagatgc tgacgctggc
    11701 gcgtcgcgaa ctgtgggatc gaccggacca gtaccgcatc gatggccggt ggttgagcaa
    11761 gaacgatgtc cggctgctga aggtgccgga ggtcatcgcc ttcgagcgca cggcgcagct
    11821 gcacggctca tgggtgccgg acgcgttgct ggaggcgagc cggcaggcgc gccgccacgc
    11881 ggtgctcaac taccacatgt atcacctgaa gatgattcac gaagccgacc ggatccggcg
    11941 gcgtgatctg tacaagtcgc tggatccgga cggccggttc caggccgagg gctacgacta
    12001 tctgaccgaa gccggtgatg aactcgaact ggcgcccatc accgaagatc gctggtacga
    12061 ccggcgtacc ctgccggccg atctgcaggc catgctcgaa cggtcaacgc ccgtgggcag
    12121 cccgacgctg cgtgccaccg cagggcgctc acttttcgat gatccactgg atcacgccct
    12181 tggcgatgaa gccgaccatg ccgaaggcca gcgccagaaa cagcacgaag gtgccgaact
    12241 tgccggcttt ggactcccac gccagctggc cgatgatgaa cagcatgtag aggatgaagg
    12301 cgctgacgcc gaaggtcagg ccgaaccagg cgatctcctc ctcgttgaag ccgaacatca
    12361 ggcgcctcct tcgggcggca ggcgctcggg caggctgtcg gcgtcgacca gcgcgcggta
    12421 ggcgtgccag ctggcatggc ccagccaggg cagggccagc accagaccga gcagcacggt
    12481 ggccatgccc agcagggtca gcagcatgat gagcgcggcc cacagagcca gcggcgcggg
    12541 gttgttcagc accgcctgcc agctggtcag caccgcctgc agcacgtcgg cacgccggtc
    12601 cagcagcaac ggcatggcga ccacgctgga ggcgaacagt ggcgccgcca tcagggcgcc
    12661 cagcatcagc cacagctcga acaggtggcc gccgggcgcc agcaccacgt ggcgcagaaa
    12721 gtcgagcggc gtgtgcaccg gctgcggcgc cagcgcggtg atgagcgcgg ccgaggtcag
    12781 cacccagccg ctgccggcca gcgccagcag caggccgaag cgcaccaggc tccagtagcc
    12841 gccatgcacg gcgtagcgcg agtactgcca gcgtatccag gtcttgcgca cggtggcggc
    12901 gttggccggc tcgccgcgct cgagcgcgcg cgacagcgcg taccagccgg tggccagcac
    12961 cggtgcgatc accagaaagc cggaaaaagc cccggccagc agccagaact ggtcggcggc
    13021 cagcgccagc agcagcccac cgaagccggc cagcagggcg ccgtacagca ggctgagcgg
    13081 gccgacgcgc cacaggtcgc gccagcccag ggccagccag tgcagcggtg cgtccagggc
    13141 gaccgtgcgc acggccggca gggtcagggt gccgcgcgcc gggcgcggcg gcaggggggg
    13201 tgggaggtgg gtatggttca cgcgcagcag cttaagggca gtgggtcgcc cgtgccagcc
    13261 tggcggtggg cgacgacatc aaaaagtata gctgctggcg cttgatggac aagcgctgaa
    13321 tgccattctg acgtggaacg gcagaacccg ccggcgggcg ccggcgggcg gtgcgccggc
    13381 gtcagcgcgg gcccggcccc atgcccgggc cccagccgcg gtgttcgcgg tggccggcgt
    13441 gccagccggc gccgtggcgg ccgcgcgcgg cgaggcgatc gaaactctgc tgctgctcgg
    13501 gcgtgagctg ggcgtagaag gccttggtgg cgtcgccgcg ctgcttcatg cgggcgtggc
    13561 gttcgtcggc gcgcttttgc atcaggtcga tgcgctcggg cgtgctgagc ttggcgaagt
    13621 cggcgcgcgc gccggggccg cgtggctcga acgccggcgg ctgcatggcg gtgctgaact
    13681 gcgtccaggc gccttcctgg gtcgggttga gcttgagttc ggccttcagc ttgtccatgc
    13741 gctgggcgtg gcgctcggcc atgcgctgct gcatgcgggc cgggtccatg cgttcggcgc
    13801 ggcgctcgct ccagccgggg cgggccgggg cgtcaccggc ggcgggtgcc tgggccaggg
    13861 ccagggtgcc ggtggctgcc agcaggctgg ccagcagaag gtgtttgcgg tgcagattca
    13921 tgcgacggtc ctttcggttg cgggacccgc gcgggcgggt cgggccgagc cggggcggga
    13981 tgccgcggcg ttccggttgc caggcagtgt gcgccgccca tgtgtcggcc gcttggcccg
    14041 cgcatgtcgg tttgtaaagc tgtgcgaagt ccggccccgg cggccggttc agccgctggt
    14101 gccggccagg tcgtcgagga tcgggcagtc ggggcgcgcg tcgccgtggc agtggcacac
    14161 cagctggtcc agcgtgcgcc gcatgtcctg cagggcggcg atgcgctgct ccagctcggc
    14221 cagatggcgc tgggcgatgc gcttgacgtc ggcgctggcg cggctgcggt cgtgccagag
    14281 gctgaccagc tcggcgattt cggccatgga aaagcccaga tcgcgcgcgc ggcggatgaa
    14341 gcgcagggtg tggacgtcgg ccgcgccgta ctggcggtag ccgctgtcgg tgcgcgccac
    14401 ctggcccagc aggcccagcg attcatagtg gcgcaccatc ttggccgaca cgcccgaggc
    14461 gcgcgccgcc tcgccgatgc cgatcgggcc gctggcgggc gtcgcgctca tgccgccacg
    14521 tcgtagcctt cttcgcggat ggcggcggcc agggcctcgc gcgattgctg gctgccgctg
    14581 acttcgaccc tgttctggct gcggtcgatt tgcacctggg cgccggggtc gagctgcagc
    14641 agcgcgcgct tgacgctggc ctcgcaatgg ccgcaggtca tgccggtgac ggtgaaggtc
    14701 tggttcatgg aatgctccgt tcaaaaaacg ccatgatgaa ccttgccatg atggtaatgt
    14761 caagggcttt cgggagggcg cgaccatccc tacgcagagg acgcttgaca ttgccatcat
    14821 ggcagggttc agactggcgg gcatgaacac caccactgct tcttccacgg tcgatctggg
    14881 tgtcggcggc atgacctgcg ccagctgtgt ggcgcgcgtc gaaaaggcgc tgcagaaggt
    14941 gcccggcgtc gacagcgccg cggtcaacct ggccaccgaa tcggcgcgcg tgcacttcgt
    15001 cggcgacgcc ggcgagctgg gcccgctgct gcgccgcgcg gtgcgcgacg ccggctacga
    15061 gccgcgcgcc gccgaggccg cgctggccga gcgcgagggc gggccctggg ccggctttct
    15121 gccggtcggc atcggcctgc tgctgtcggc gccgctggtg ctgcccatgc tgggcatgcc
    15181 gttcggctgg cactggatgc cgccgccctg ggtgcagttt ctgctggcca cgccggtgca
    15241 gttctggctg ggcgcgcgct tttaccgcgc cgcctggcac gcgctgaagg cgcgcagcgg
    15301 caacatggag ctgctggtgt cgatcggcac cacggcggcc tggctgctgt cgatgtggct
    15361 gtggctgggc aagtcgcatc cgccggggca tgagccgcac ctgtatttcg agggtgcggc
    15421 ggtggtcatc accctggtgc gcctgggcaa gtggctggaa gagcgcgcca agcgccagac
    15481 cacggccgcc atccgcgcgc tgcacgcgct gcggcccgag gtggcgcacc tgatcgactg
    15541 ggatggcagc gaaaaggaca tcccggtgga cgagctgcgc gccggcgacc gcctggtggt
    15601 gcgcccgggc gagcgcgtgc cggccgacgg tcggctggcc gaaggcgcca cccaggtgga
    15661 cgagtccatg ctgaccggcg agccgctgcc ggtggccaag gcggtgggcg acgcgctgac
    15721 cggcggctcc atcaacggcg agggccgggt ggtgctgcgc gtcaccgccg ccggtgccga
    15781 aagcgtgctg gcccacatca tccgcctggt gcaggacgcg caggcggtca aggcgccggt
    15841 gcagcggctg gtggatcagg tggcggcggt gttcgtgccg gtggtgctgc tgatcgcgct
    15901 ggccaccctg ctgggctggt ggctgctggg cggcaccggg ctggaggtgg ccatcatgcg
    15961 cgcggtggcg gtgctggtga tcgcctgccc ctgcgcgctg ggcctggcca cgcccaccgc
    16021 catcatggcc ggcaccgggg tggcggcgaa attcggcatc ctgatcaagg acgctgaggc
    16081 gctggagatc gcccaccgcg tgcgcaccgt ggccttcgac aagaccggca ccctgaccgt
    16141 gggtcagccg cgcctgctgg cgctgctgcc ggcgccgggg gtggacgagg cggccgccct
    16201 gcggctggcc gccggcctgc aaagcggcag cgaacacccg ctggcgcgcg ccgtgctggc
    16261 gcaggcgggc gcgcgcggcc tgtggccacc ggccgagggc acggcggccg agctggccga
    16321 gctgcgcgcc gtgcccggcc gcggcaccga aggccgggtg gggcagcagc gggtgcgcat
    16381 cggcagcgcc cgctggatgg ccgagctggg cgtagatttg caggttttcg aggccctggc
    16441 gcaggcgcag caagcgcaag gcgctactgt ttcgatactg gccacgcagc cggccgaggc
    16501 ccctgaaggc accccgccgc gggccgtggc cttgctggcc tttggcgacg agcccaagcc
    16561 cggcgccgct gccgcgctgg ccgagctgca cgcgcgcggc atccgcgccg tcatgatttc
    16621 gggcgacaac cgcggcgccg ccgaggccat ggcgcgccgc ctgggtctga aacccgagga
    16681 cggcgaggtg ctggccgagg tgctgcccgg cgacaaggcg caggcggtgc ggcagctgcg
    16741 cgccggtggt gccgtggtcg gcatggtggg cgacggagtc aacgacgcgc cggcgctggc
    16801 ggcggccgat gtgggcatgg ccatgggcag cggcaccgac gtggccatgc acgcggccgg
    16861 catcaccctg atgcgtggcg acgtcggcct ggtgccggcg gcgctggaca tttcgcagcg
    16921 caccgtggcc aagatccggc agaacctgtt ctgggcctcc ttctacaacg ccgccggcat
    16981 cccgctggcg gcgctgggct acctgagccc ggtgatcgcc ggcgccgcca tggcgctgtc
    17041 gtcggtcagc gtgatgagca acgccctgct gctgcgccgc tggcgcccgg ccagccaggg
    17101 cagtgtaggc acgccgggct gaccgggctt gcgccggcgg cggtccggac ggacggtcgc
    17161 gggtggcccg gccccgacgg cgcaacttga cgccgatcac gtctggcgcg ccgggtggcc
    17221 actacactcg ggggccaagt ccatagcaag gagacattcg ccgtgaagca ccgaagcctg
    17281 gaaatcatcc gcgacgaaca ctcgtcgctg gcggccatgc tgcagtccat gcgcctgatg
    17341 gtcgatcgcg ggccgcagga cgatccgcgc aacttcttcg acgtgctgcg cgccatgctg
    17401 ttctacatcg acgaattccc cgagcgcctg caccatccca aggaaaccga gctgctgttt
    17461 ccgcgcgtgg cggccgccgc gcccgaggtc ggcatggcgg tggcccgcct ggaccacgac
    17521 cacgaataca ccgaaaaggc ggtgcgcgac atccagcacc tgctgctggg ctgggaactg
    17581 ctgggcgagt cgcgccgtca gcgcttcatc gacgccttcc accgttacgt caacctgtac
    17641 ctggagcaca tgcggctgga agagcaggaa gtgctgccag ccgccgaaaa gcacctgacc
    17701 gaggccgact ggcgcgagct ggacgccgag ttcgagaaga actgcgaccc gctgaccggc
    17761 aagtacaaac ccgacccggt gtacgaaaag ctgttcaccc gcatcgtgcg cgatgcgccg
    17821 gcgccgatcg gcctgggcgg cagctgaagg cccggccgct gcacctgcca gggccgcctg
    17881 cgggcggccc tgtgcatttg tggggtcatg ggcggatggg tttgaacttg ggctcgatcc
    17941 gggccataaa cgcccggtgt ccgccccggg ccagtttgtt gaatggagag ccgtcatgaa
    18001 atcccgtgcc gccgtggcct ttgccgcggg ccagccgctt gaaatcgtcg agatcgacgt
    18061 cgccccgccc aggaagggcg aggtgctggt gcgcatcacc cacaccggcg tctgccatac
    18121 cgacgccttt accctgagcg gtgatgaccc ggaaggggtg tttcccgccg tgctgggaca
    18181 cgagggcgcg ggcatcgtcg tcgaatgcgg cgaaggcgtg accagcgtca agcccggcga
    18241 ccatgtgatc ccgctgtaca ccgccgaatg cggcgaatgc ctgttctgca agagcggcaa
    18301 gaccaacctc tgcgtggccg tgcgcgccac ccagggcaag ggcgtgatgc ccgatggcac
    18361 gacgcgcttt tcctacaacg gccagcccgt ctaccactac atgggctgct ccactttcag
    18421 cgaatacacc gtggtggccg aggtgtcgct cgcaaagatt gaccccgcgg ccaaccccga
    18481 gcaggtgtgc ctgctgggct gcggcgtgac caccggcctg ggcgccgtga agaacaccgc
    18541 caaggtgcag cccggcgaca gcgtggccgt gttcggcctg ggcggcatcg gcctggccgt
    18601 ggtgcagggc gccaggctgg cgggcgccgg gcgcatcatc gccatcgaca ccaaccccgc
    18661 caagttcgac ctggcgcgca cctttggcgc caccgactgc gtcaacccca aggacttcga
    18721 caagccgatt cagcaggtga tcgtcgagat gaccggctgg ggtgtggacc atagcttcga
    18781 atgcataggc aacgtgaatg tgatgcgcgc ggcgctcgaa tgcgcgcacc gtggctgggg
    18841 ccagagcgtg atcatcggcg tggcgggcgc gggccaggag atctccaccc gccccttcca
    18901 gctcgtcacg ggccggcgct ggctgggcac ggcattcggc ggcgtcaagg gccgcagcga
    18961 actgcccggc atggtgcagg acgccatggc cggccgcatc cagctcgcgc cattcgtcac
    19021 gcacaccatg ggcctcacag acatcaacca ggccttcgac ctcatgcacg agggcaagtc
    19081 gattcgttcg gtgttgcgcc acggctgagt catcacatga caaatcgggc cacggcgctt
    19141 gtctgacaag tgccaacagc tatcgaaata ggagtattcg gggcatggaa cggatcgagc
    19201 agcacgccag cttcggcggc cgtcaggagg tctggcggca cgccagcgcg agcaccggca
    19261 cgccgatgaa cttcggcatc tatctgcccc cgcaggcgct ggcgggccag cgctgcccgg
    19321 tgctgtactg gctgtcgggg ctgacctgca gcgagcagaa cttcatcacc aaggccgggg
    19381 tgcagccgca cgccgccgcg cacggcctga tcgtggtggc gcccgacacc agcccgcgcg
    19441 ggcccggcgt ggccgacgac gcggcctacg acctgggcca gggcgcgggc ttttatctga
    19501 acgccaccca ggcgccgtgg gcggcgcatt accgcatgca ggactatgtg gccgacgagc
    19561 tgccggcgct gatcgagcgg cactttcctg ccagcgacgc gcgcggcatc ttcggccaca
    19621 gcatgggcgg gcacggcgcg ctggtgacgg cgctgcgcca cccggggcgc tatcgcagcg
    19681 tgtcggcctt ggcgccgatt gccgcgccga cccaggtgcc ctggggccac aaggcgctgg
    19741 cggcgtacct gggcgacgat ccggcggcct gggccggctg ggacgccgtg gccctgatcg
    19801 ccaccgcacg cgagcgcctg ccgctgctca tcgaccaggg cgaggcggac gaattcctgc
    19861 cgcagctgcg ccccgatctg ctgcgcgccg cttgcgaggc cgccggccac ccgctgacgc
    19921 tgcgcctgca gccgggttac gaccacagct actacttcat cgccagcttc atcggcgagc
    19981 atgtggcgca ccacgccggc gcgctgcgcg gctgatcccc aggcgccgtg cggtcatggc
    20041 gccgggtgcg gccggcgcgc gcgcggcgtg gggacaatgc aggcgccgat tccggcaccc
    20101 atcacaccac ccacgaagga tccgaaccat ggccgacacc tggctgccca ccctcatcac
    20161 cgacaccccg cagcagggtt ttgaactggc gatcacgctc agccgcaagg gcgtgcgcta
    20221 cacccagccc gaccccgagg tgctgcacaa gctgcgcccg gtgtatgcca acgacgcgca
    20281 cgagctgacc gccgcctcgc aggtgatcgc gctcaacttc cagaccgtcg ccgccgccaa
    20341 caactactgg cgcaagtgag cggcgccgcg ctggtcgttt cacccatgaa cgcggttttg
    20401 caatgtccaa tttcgggctg ccgaggccaa aggttcaatt cgccagcgcc ggatagtcgg
    20461 tgtatccgtg cgcgccgccg ccgtacatgg tggcgcgctc cagcccctgg tagggcccgc
    20521 cgcggcggat gcgggcggcc aggtcgggca tgctgatgaa ggccttgccg aacgccacca
    20581 ggtcggcgta gccgctggcc agggcggcct cggccgactg cgggtcgtag ccgttgttga
    20641 cgatccaggc gccctgaccg ccggcggcgc ggtaggcggc cttcatggcg gcgtagtcga
    20701 acggtcggcc ctcgacctcg cgcgggccgc cggtggcgcc ttcaatcacg tggatgtagg
    20761 ccaatcccag cggcgccagc tggcgcatca ggtgctcgaa cagcggctgc gggtcggcgt
    20821 cgacgatgtc gttggccggt gtgacgggcg ataggcggat gccgacgcga tcggcgccga
    20881 cggcgtcgca gacggcgcgg gcgacttcca gcatcaggcg cgcccggtgg gcgatgcggc
    20941 cgccgtagtc gtcggtgcgg tggttggcgc cgctcttgag gaactggtcc agcaggtagc
    21001 cgttggcgcc gtggatttcc acgccgtcga agccggccga ttgcacggca tggcgcgcgg
    21061 cggcggcgta gtcgtgcacg atgccgggga tttcgtggcg gtgcagcgcg cgcggcatgc
    21121 tggtgggcgc gaaatgacca tggccctggg cgtcgaagac gtaggtcttg gcgttggcgc
    21181 gcagcgccga aggcgccacc gggtcggcgt ggccgggttg cagcacatgg tgcgacacgc
    21241 gccccacatg ccagagctgg cagacgatgc ggccgccgcg cgcatgcacg gcctgcgtca
    21301 cctgcttcca gccgtcgatc tgttcgctgc cgtacaggcc cggcacgtcg gcgtagccct
    21361 ggccctgcgg gctgatggcc gtgccttcgc tgacgatcag gccggcgccg ctcagcgggt
    21421 cggcgcgctg ggcgtagtac tcggccatca gcgcattggg aatggccccg ggcgcgcggt
    21481 tgcgggtgag cggcgccatg acgagccggc tggcgacgtc gatggcgccg acgcgggtgg
    21541 cggtgaacag ggtctgggcc atgggtgggt ctcgcggtca ggggctgcct gggccgattg
    21601 tgcgacaggg gggcgcgagt gcgctgcctg tccccgccgg gccggcaggc agggcctgcg
    21661 cggcgcacaa tcgccccatg agcccgcccg ccccgcccct gtcgcgcctg ctgtggctgg
    21721 cgctggcgct gtcgctgggc gcggcggtgt cgctgggcat cacgcgcttt gcctatgcgc
    21781 tgctgctgcc ggtgatgcgc gaggacctgg cctggagcta caccctggcc ggcgccatga
    21841 acaccgccaa cgcgctgggc tatctgctgg gggcgatcgc cacgccctgg ctgatgcggc
    21901 gctggagcgc ctcgacggtg ctgctgacgg gcagtgcgct ggccagtgtg ttcatggcgg
    21961 cgtcgggttt cttcaccgac gccgtgccgc tgctgctgca gcgcctgctg gccggcgcgg
    22021 ccagcgcgct ggtgttcgtc tcgggcggtc tgatggcggc gcggctgggg gcgcagtcgc
    22081 cgcagcgggc cgggctgctg ctggggctgt actacggtgg cacgggctgg ggcatcgcgg
    22141 cgtcggcgct gggcgtgccg ctggtgctgc aggccgcgca gggcgtggcg cacggctggg
    22201 cctgggcctg gtgggggctg gcggcgctgt gcctggcggc cacggcggtg ctctggtggc
    22261 cggccaatgc gctacgaaaa caggagctaa tggcgcacgg tgcaagccat ccgcaggccg
    22321 attcacccca aaacctgcca tggcgcgcga tggccctggc gctggccggc tacacgctgt
    22381 tcggcgtcgg ctacatcggc tacatgacct tcgtcatcgc gctgctgcgc gagcagggcg
    22441 tgagcgccgg cgccgtcacg ttgttctaca ccctgctggg gctggcggtg gtggggtcgg
    22501 cgcggctgtg ggccggcctg ctggatcgcg cccagggcgg cggcgcgctg gcgctgctga
    22561 acgccctgct gggcgtggcc acggtgctgc cggcgttgac cggggcctgg ccggtggtgc
    22621 tggcgtcggg gctgctgttc ggggcggtgt ttttgtcgct ggtggcgtcg accaccgcct
    22681 tcgtgcgcca caacctgccg ccggcgcagt gggcggcggg catcagcgcc ttcaccatcc
    22741 tgtttgcctt cggccagatc gtcggcccca ccgtggtggg ctggatcgcc gacgggccgg
    22801 gcgggctggc gcagggcctg cgctactcgg ccggcgcgct gtggctgggg gcgctgctgg
    22861 cctggcgcca gcggccgctg gcgcgcgccg cctgaacccg caagctcaga tcagcccctc
    22921 ggccttcatg gccgcctgca ccgccgggcg ggcggccacg cgctcgcgcc aggccagcag
    22981 gttcgggtag ggcgccaggt cgatggccat cggcttggcc cagtgggtga cggtgaacag
    23041 gtaggcgtcg gccacgctgt agtcgctgcc catcacgaag gccttgccgg ccagctcgcc
    23101 gtcgacgaac ttcaggcgct cctgcaggcg ggccagggcg acctgcttgg cctcggccgg
    23161 catggccggg ttgaacagcg gcgaaaagcc cttgtgcagc tcggtggcgg tgaaatgcat
    23221 ccacatctgc agctggtagc gcgccatgct gccgcagggc gggatcagcg ccttggccgg
    23281 cgcctggtcg gccacgtact ggcagatgat ggcgcattcg cgcagcgcct ggccgtcgtc
    23341 gagcaccagg tagggcacgt agcccagcgg gttgacctgg cggtagtcgg agccgtcggg
    23401 cagctgcttg gtcttggtgg gggccgagat ggcttcgtac ttcaggccgg cttcttccag
    23461 cgcgatatgg gcggccagcg aacaggcgcc ggcggagtag tagagcttca tgcgggtctc
    23521 ctggggggtg aagggatgcg tcgtgcccgg accgggcctg ggcagggcgt ggcagcttag
    23581 caagcgccgg gcaaccttgg ggcggctgcg ggttcagccg ccgcggccgg tggctcgctc
    23641 cagcgtgctc aggcagcgca gggcgcgcgc gccgccgtcg tccggcgggc cgcacatttc
    23701 gggcgagggc cgcaggctgg cgcagacggc ggggccttcg ggtcgcccga acagccggca
    23761 gcggccttgc ggtccggttg caggcatggc ggccacccac gacgctataa aaacagaagc
    23821 tttcagcgca tgacagtcgg gtgccagggg ccaatctcat ggatagagac cgcgcagatc
    23881 gcgggccgac aggctgcacg tgcaggcgac gatggaggtg gcgctgcgca ggctcacagg
    23941 gtgctcttgc gcggtgtccc cgacggcccg cttcatgcag tgcgccggac gcgtggcacg
    24001 gcttcgaaac cgacgtggct cacattcccg agcgtcacgg atctggtggc gcggtccgaa
    24061 acatcggcgc gtcgatcacc gcaagaggtt cacaccacct tggccggcgt ggggcgacga
    24121 gctgccgtgc attttcttcc tccgcagaag taccaagagc cggacaagct gtccggctcc
    24181 tggatccaac ggcgtgctcc tgattaacgc cgggtgccgc gccgtgctct caacggactg
    24241 gccgcggcca gcagcatcaa catggccagc gccatcagca cgtactccga agtggtcgga
    24301 atcggtgcca cgcccgccgc cgctgcggtg acgccgatgg cgcccggatc ggtgatggtg
    24361 ccgttggcgg ccaggtcgtt gtcacccagt tgaccatcgg tgatcgtgaa gctcacctgg
    24421 ttgccgctga tcgtggccgg gatggtgtag taatggtcgg tggtgttgcc gggctccttg
    24481 ccccacttgt agtactgcgc gcccggcggg atggcggtgg gataggtgat ggtcaccgtc
    24541 accgccccgt tggcggccgg gcagctggtg gccgtgaagc cgaagaagcc gtgcgggaag
    24601 ctgacgcccg caggaggcgg cgctggcgtg ccgacgaagc cactgccggc agcaaagccg
    24661 caggcggcgc caccaccagt gagggtgccc gtggcggtgc cggtacccgt gctggtgggt
    24721 ccggagatca tgttcacgca ggacaccgcc acgttggtga cgttggccga cgcgaccgtg
    24781 cccgtgccat tgctgacggt gcagacctga ccgaccggct gggtgccgac ggtcaccgca
    24841 taggcgctgc cgctggtcag cgcggtggcg aaggtgaagc tgccgttggc attgacggtg
    24901 agcgcgtcac caccgttgtt gagcaggccg accgtcttgc cggtgaccag gccggacacg
    24961 ctgccaccga tggtgtaggt cggcacgttg acgcagttca ccgccacgtt ggtgacgttg
    25021 gccgacgcga ccgtgcccgt gccgttgctg acggagcagg tctggccgac cggctgggtg
    25081 ccgacggtga cggcgtaggc cgcgccattg gccagcgggg tggcgaaggt gaagctgccg
    25141 ttggcgttgc gcgtcagtgc gtcgccgccg ttgttgagca gcaccaccga gttgcctgcg
    25201 cccaggccgg atacgctgcc gccgatggtg taggtgttgg tggtgcagtt gacggtcacg
    25261 ttggtgacgt tggatgcaac caccgtgccc gcaccattgc tgacggagca ggtctggccg
    25321 accggttggg tgcccacggt cacggaatag gtggcgccac tggcaatggg cgtggcgaag
    25381 gtgaagctgc cgttggcgtt gcgcgtcagc gcgtcaccgc cgttgttgag cagcaccacc
    25441 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgccgttg gcgttgcgcg
    25561 tcagcgcgtc accgccgttg ttgagcagca ccaccgactt gcccgcgccc aggccggaca
    25621 cgctgccgcc gatggtgtag gtgaccggtg gcgcgttgac gcagttgacc gccacgttgg
    25681 taacgttggc cgaccctacg gtgccgctgc cattgctcac ggtgcaggtc tggcccacgg
    25741 gctgggtgcc cacggtgacc gaataggtgg cgccactggc aatgggcgtg gcgaaggtga
    25801 agctgccgtt ggcgttgcgc gtcagcgcgt caccgccgtt gttgagcagc accaccgagt
    25861 tgcccgcgcc caggccggac acgctgccgc cgatggtgta ggtgttgctg gtgcagttga
    25921 cggtcacgtt ggtgacgttg gccgaggcga ccgtgcccgt gccgttgctg acggtgcagg
    25981 tctggccgac cggctgggtg ccgacggtga cggcgtaggc cgcgccattg gccagcgggg
    26041 tggcgaaggt gaagctgccg ttggcgttgc gcgtcagtgc gtcaccgccg ttgttgagca
    26101 acaccaccga tttgcccgca cccagaccgg acacgttgcc gccgatggtg aagctgctca
    26161 gcgaggccgg caccatgacg atgccgacgc ctgaatcccg gtcccagccg gcggcctcga
    26221 tgtcgagcgc cgacgcgttc atggccgcca cgatctgggc gttggtccag cccgggttgg
    26281 cgcccttgat cagggcggcg atggccgccg ccgtgggtgc ggctgccgag gtaccgcaga
    26341 aagtggtgaa ccccggggtg gtggtggtgc cacagtcgcc tgccgccatg tcgaccttcg
    26401 gcaacgtggt gccgccattg gtggcgaaca gcaggttgcc gggcgtgatg gcggtaccgt
    26461 tggggtggta gaagatcttg cgcggaccgt ccgaactata ggtttccacc gggttggccg
    26521 cgccgccggt gaaggtgccg cccgggccgg tggcggtggt cagggacacg gcggcgaccg
    26581 aaatgccgct ggccgctgcg ttgtggccaa aggtgctgcc ggcggtgctg tcggccggcg
    26641 tgatgcgacc acggtgggta tccaggcgca gtgcgcgggc agatccgctg tagcgcacga
    26701 tgtaaatccg cgagccgatg gggtacgggg tgccgctgga ggcgagttcg taggggtcct
    26761 gggtgccggt ctggttgcct gtgctttgcc ccagcaccac agtgccggcc gcgttggtca
    26821 cgatcaggtc gtagtcgttg ctggagccgc ccagggggtc gctccatttg agcgacacat
    26881 atcttgtctg cgcagtcagc gtggtgtagt tgttgctgcc aaacgcatgg agttgaatgg
    26941 acgcggcgcc atagatgctc tggatggcgg acggaatggg gttgccgctg gcgacgaagt
    27001 caccttcaaa ggtgccggac tggttgtcgt tcttgttgcc ggagttggcg gccgaggaga
    27061 agtacagcgc tccggcggcg gtgacctggt tcacggcctg ggcgatcggg ccgtcctgaa
    27121 acgcgccttc gttgaagtac gtgacgtcgt caacgatgat gttgcagccg gcggcctgga
    27181 gattgacgat gttggtggcg aacgaagcga cgctggtgaa agcggtggcg tagcccagtg
    27241 atgcgcccgg cgccatgtcg tgcacgattt ccagcatcgc cgtgccttcg tctcccgaac
    27301 cggcctggcc gggcagtacc gtcacggtgg ccggcagatc gcccgtggcc tggcgcgcgg
    27361 ccagcgaatt gacaccgtcc gacagcacgc agaccatggt gcctacaccg gtgcggccgg
    27421 cgctgtgcgc ggcattggcc ttatgggcgg ccacgccttc ccaggtggcg ctaccggcgt
    27481 tggtcgctgc accgggctgg gggaggttct tcagtttttc gcgcagaacc tcgggcggca
    27541 attcaaggcg gttggtggtg gcttcctcgc gcgggccgac ggtacgcaca tcgggccggt
    27601 tggcaatggc gctcaactcc gacaccggta cgcgggcggt gatggtgtcg tactgcggaa
    27661 acgccgagag ctcggtgccg ccgcgcgatc tgatgaacgc ggtcaacgtg tccgacacct
    27721 tacccttgat gaccacgaaa atggtctggt caggcgcgac atgacgctcg gcgaagtcct
    27781 gcacataggc tggcagcgcc tgccggctgg cggcgttgcg cagctggcct gaaccggcgg
    27841 cgcgcgtcag atcgatcagc gc
//
LOCUS       DAOQTZ010000023        27820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2016_length_27820_cov_13.852980, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000023 DAOQTZ010000000
VERSION     DAOQTZ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 27820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27820
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2016_length_27820_cov_13.852980"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PKN51_04440"
     CDS             complement(<1..775)
                     /locus_tag="PKN51_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNT84380.1"
                     /translation="MKNEWFSKIAIWMVIAMVLFTVFKQFDGGRMASANHMAYSDFLE
                     EVRNNRVKQATIQEGQGGTDIVAVMSDDRRVRTQATYLDRGLVGDLINHNVKFDVKPR
                     EEGSLLMTLLVSWGPMLLLIGVWIYFMRQMQGGGKGGAFSFGKSKARMLDENNNTITF
                     ADVAGCDEAKEEVKEVVDFLKDPQKFQKLGGRIPRGLLLVGPPGTGKTLLAKSIAGEA
                     KVPFFSISGSDFVEMFVGVGAARVRDMFENAKKNAPCIIF"
     gene            complement(958..1629)
                     /locus_tag="PKN51_04445"
     CDS             complement(958..1629)
                     /locus_tag="PKN51_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNT84381.1"
                     /translation="MKVKSKSKKVNKAWLADHINDPYVKLAQKEGYRARAAYKLKEID
                     ETLGLIRPGMRVVDLGSSPGAWSQYLRRRLAPAGAAVGALDGTIVALDILPMEPIEGV
                     HFLQGDFRDDEVLARLNEAVGGQPLDLVVSDMAPNLSGVESSDAARIAHLVELALDFA
                     LGHLKPGGALVVKLFHGSGYSQLVKLFKDSFKVVKPVKPKASRDRSAETFLVGIGPKP
                     PAETG"
     gene            1725..2198
                     /locus_tag="PKN51_04450"
     CDS             1725..2198
                     /locus_tag="PKN51_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HNT84382.1"
                     /translation="MPHIQLTPAERKAHRAEAHHLDPVVMVGADGLTPAVVKETDAAL
                     NAHGLIKLRVLGDDRAAREAMFQQLADQLNAAPIQHIGKLLVLWRPKPAKPRSLDEER
                     RPGPKDVKVIKHSKRGGQRPEVKVVRVLGNQRLTPGGKVKRAQPKQKSVKKGRAD"
     gene            2266..2988
                     /locus_tag="PKN51_04455"
     CDS             2266..2988
                     /locus_tag="PKN51_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT84383.1"
                     /translation="MKWGTKYGPEYVNRLYGMVRRHLAGEFQFICLTDDASGVRPEVR
                     CLPIPPLNLQLKPGQRDGAWKKLTTFEADLHGLTGTALFLDLDVVVVGPLDDFFTQPG
                     EFLIIHDYPRFWRFGERVVGNSSVYRFALGAHADVLAYFRTHMDEVQKQYRNEQDFLS
                     HFLHRQGKLNYWPAAWCPSFKYHSIPTWPTNYWKAPIVPPGARIVIFHGEVNPPDALA
                     GKRNKRFAFIKPALWVADAWQG"
     gene            complement(3071..3964)
                     /locus_tag="PKN51_04460"
     CDS             complement(3071..3964)
                     /locus_tag="PKN51_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HNT84384.1"
                     /translation="MDAPIVELTLNDWGQASSRPEWTAAVEAGKVLYFPQLRFQLEGH
                     ELALLREDMLAAGTRNVSLGADGVLKGAGGSADEQRALAAMIGRFRGQALQLVDGLLP
                     AYRGLLRVAPTSFRPRQVETRAQSIRADDQRLHVDAFPSRPNYGERILRVFANINPQG
                     VPRVWRVGEDFETVARQFLPRAKPYRLWQARALNALHVTKSLRSEYDHLMLQLHDLMK
                     RDERYQQSGGQVSMPFAAGSAWVCFSDQATHAVMSGQFMMEQTLHLPPGREANPQASP
                     LAILSRLVGRPLVGVGAGTPR"
     gene            complement(3969..4385)
                     /locus_tag="PKN51_04465"
     CDS             complement(3969..4385)
                     /locus_tag="PKN51_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNT84385.1"
                     /translation="MSDRLALLAAALWWGTLSAVIGFVVPLLFRLLPSKALAGQTAAV
                     VFTAQAWVSLVCGALLLLLLRGRAGAAGEVRARTTLGLVMAGMLLALLIEFAVAPRIV
                     ARVNLPLWHGLGTAMFALQWLCAGVVLWRLGGTPRR"
     gene            complement(4397..4873)
                     /gene="greA"
                     /locus_tag="PKN51_04470"
     CDS             complement(4397..4873)
                     /gene="greA"
                     /locus_tag="PKN51_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNT84386.1"
                     /translation="MATFPITKRGAEMLKAELHRLKTKDRPEVINAIAEARAQGDLSE
                     NAEYEAAKDRQGFIEGRIKEVEGKLAAAQVIDPAGLDAGGRIVFGATVELEDEDSGEQ
                     VTYQIVGEDEADLKHGLINVGSPIARALIGKEEGDTAEVQAPGGVRHYEVIAVRYV"
     gene            5021..5728
                     /locus_tag="PKN51_04475"
     CDS             5021..5728
                     /locus_tag="PKN51_04475"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNT84387.1"
                     /translation="MIQPTRRHLLHASAAMATTALAGRALAQDSCAVPTRDVQAAISP
                     DAALQRLREGNARFVAGQPIQCDLRAQVRASAGGQAPFAAVLGCIDSRVPPELVFDQR
                     LGDIFAARVAGNFVNDDILGSLEFATQLAGARLIVVLGHTECGAIKGAVDNAKLGHLT
                     GLLAQIRPSLERLDYRGTPSSKDKALVQRVAERNVQDAVTRLNAAPVLAALVKAGQLR
                     IVGAMHDVATGRIGWQV"
     gene            complement(5801..6775)
                     /locus_tag="PKN51_04480"
     CDS             complement(5801..6775)
                     /locus_tag="PKN51_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT84388.1"
                     /translation="MGIKSVLAFLALGLAAMASAQTANYPNRPVTLIVPAGAGGGTDV
                     VARVLADELGKRLGQAVVVDNKTGASGMLGTQAVARAAPDGHTLLVAYSTPIFYAHHI
                     FAKVPYDIRKDFEFISQLAATSLVMMVNADVPAKNMKEFMAWAQQNKGKVNYGSYGQG
                     SPGHLMSAYLSDSRKLDMTHVAYKSEAPHIQDLAGGVVSWGLGTIAAAQGALKDKRIR
                     PIAIYGPKRLAELPDVATMAEQGFADPEFNTVAWFTLLAPAGTPKPILQRLEKETVEI
                     IHSTAMKARFQVFGLESVPGGAARFHKDFDAVDPVIQKLVRISGVKAN"
     gene            complement(6956..10237)
                     /gene="carB"
                     /locus_tag="PKN51_04485"
     CDS             complement(6956..10237)
                     /gene="carB"
                     /locus_tag="PKN51_04485"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNT84389.1"
                     /translation="MPKRTDIQSILIIGAGPIVIGQACEFDYSGVQACKALREEGYRV
                     ILINSNPATIMTDPATADATYIEPITWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     WRHGVLAKYNVELIGARPEAIDKAEDRLKFKDAMTRIGLESARSGIAHSMDEAWAVQK
                     RVGFPTVIRPSFTLGGTGGGVAYNPEEFETICKRGLEASPTNELLIEESLLGWKEFEM
                     EVIRDKADNCIIICSIENLDPMGVHTGDSITVAPAQTLTDKEYQLMRNASLAVLREIG
                     VDTGGSNVQFAVNPADGRMIIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNDITGGATPASFEPTIDYVVTKIPRFAFEKFPAADSHLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEVGVDGMNEKTQDREWLEKELGEPGPDRIWFVGDAFAAGWSLDEVHQL
                     TRIDKWFLVQIEEIVKIELELDRHTEQQGDRALAALSKAELLALKQKGFSDRRLAKLL
                     KTSDKAVRDARRALGVRPVYKRVDTCAAEFATHTAYLYSTYEDECEAEPTDRKKIMVL
                     GGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTL
                     EDVLEIVDKEKPLGVIVQYGGQTPLKLALGLEAEGVPIIGTTPDMIDAAEDRERFQQL
                     LHELNLRQPPNATARTEAEALAKASELGYPLVVRPSYVLGGRAMEIVHEQRDLERYMR
                     EAVKVSNDSPVLLDRFLSDAIECDVDAVRDSTGRVFIGGVMEHIEQAGVHSGDSACSL
                     PPYYLSAATVAELKRQTAAMAQGLNVVGLMNVQFAIQEKPLEGGGKQDVIYVLEVNPR
                     ASRTVPFVSKATGVQLAKVAARCMAGQTLDAQGIGAEVTPPYFSVKEAVFPFVKFPGV
                     DTILGPEMKSTGEVMGVGKTFGEAYIKAQLGAGERLPRPLRADGTPAGKVFLTVKNSD
                     KPQAVQIARELAGMGFGLVATRGTAQAIRDAGIACETVNKVTEGRPHIVDMVKNDDIS
                     LVINTVEERRNAITDSRAIRTSALAARVPTITTIFGAEAAVEGMKCMDQLSVISVQEM
                     HAQLA"
     gene            complement(10230..10592)
                     /locus_tag="PKN51_04490"
     CDS             complement(10230..10592)
                     /locus_tag="PKN51_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84390.1"
                     /translation="MKLYAFPQATLEKAIARRMLGLASPHREWFADRWSQKPYKKSFL
                     EHKAMPLVTLLAKGKTWTDEEWDRELAAWDVRFYDAEAEVLRPLIEGDGLIQLMQKNM
                     PPQRAQALLDKLNHDRHA"
     gene            complement(10589..11800)
                     /gene="carA"
                     /locus_tag="PKN51_04495"
     CDS             complement(10589..11800)
                     /gene="carA"
                     /locus_tag="PKN51_04495"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNT84391.1"
                     /translation="MLSSLQGRFPPAILALADGSVFIGQSIGASGTSVGEVVFNTAMT
                     GYQEILTDPSYCRQIVTLTYPHIGNVGVNAEDVESARVQAAGLVIKDLPLLVSNFRAE
                     RSLSEYLRAEGTVAIAGIDTRKLTRLLRTHGAQNGAIVGLAAGEAVTPERVAATVAAA
                     REAPAMTGLDLARVVSTDRAYEWNQTEWHLGAGFGTQQAPRFHVVAYDFGVKKNILRM
                     LAARGCRVTVVPAQTPAAQVLALQPDGVFLSNGPGDPEPCDYAIAAARTLIDSGKPVF
                     GICLGHQIMALASGARTYKMKFGHHGANHPVKDLDDGRVSITSQNHGFAVDAATLPAN
                     LRPTHVSLFDGSLQGLSFTDQPAFCFQGHPEALPGPHDVGYLFDRFIQNMEQTGAGTA
                     PNAIQTGVATA"
     gene            complement(11988..12764)
                     /locus_tag="PKN51_04500"
     CDS             complement(11988..12764)
                     /locus_tag="PKN51_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNT84392.1"
                     /translation="MRPAEPLRVQSRDNALFKDLRRLAQDSGAWRRQGRLWVEGDHLC
                     RAALARGWRPELAVFSESFWPLAPAQLAQAAIKNVVFADGLMGALSALESPPPMAFVL
                     PLPAAPALAPDAPTVVLDRVQDAGNVGSILRSAAAFGFTQVAALSGTAGLWSPKVLRA
                     GMGAHFGLRLVDALTVPELAALRVPLLVTSSHQGQWLHRASLPWPCAWVLGHEGQGVQ
                     PALVERAALALRIAQPGGEESLGVAAAAAICLHASAVARP"
     gene            complement(12761..13396)
                     /gene="rnhB"
                     /locus_tag="PKN51_04505"
     CDS             complement(12761..13396)
                     /gene="rnhB"
                     /locus_tag="PKN51_04505"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNT84393.1"
                     /translation="MRSKHFLRAEQAALDWDTPGLLAGVDEAGRGPLAGPVVAAAVIL
                     DPRQPIAGLADSKKLTARRRERLYDEIRAKALCCAVAEASVEEIDRLNILQATLLAMQ
                     RAVAGLRLPPAKVLIDGNRLPQLAVRAEPVVGGDARVPAISAASILAKVQRDRWCAEV
                     DAAWPQYGFAAHKGYGTAAHLAALRAHGACPWHRRSFAPVAAAIARAGCEA"
     gene            complement(13368..14594)
                     /gene="lpxB"
                     /locus_tag="PKN51_04510"
     CDS             complement(13368..14594)
                     /gene="lpxB"
                     /locus_tag="PKN51_04510"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNT84394.1"
                     /translation="MGAPVRVAAAPRFALVAGEASGDLLAGHLLDALRARWPGLQAMG
                     VGGAEMAARGFQAWWPVDRLAVRGYLEVLPRLPALLRLRRQLGDRLLAERPDLFIGVD
                     APDFNFDLEARLKAGGIRTLHFVSPSFWAWRPEKLLKLKAAADHVLCLFPFEPALLAE
                     QGIAASYVGHPLASAIPLQSDRAAARRALGLDAQSPVVALLPGSRAAEIKHLASRFFQ
                     AAARIRQARPATQFIVPTVPERLPQVQALAAASGLGASLAVLRGQSHAALAACDVTLI
                     ASGTATLEAALFKRPMVIAYAMPALSYRLMRARRQLPWVGLPNILCAPPEWLRRPAGL
                     TAQQAADRPDAPFVVPELLQHAATPAALAQAVLDWLAAPEKIAATEARFAALHHDLRR
                     DTARLATDAIEALFAR"
     gene            complement(14599..15387)
                     /gene="lpxA"
                     /locus_tag="PKN51_04515"
     CDS             complement(14599..15387)
                     /gene="lpxA"
                     /locus_tag="PKN51_04515"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNT84395.1"
                     /translation="MPQIHPTALVDPRAELDPTVEVGPYAVIGPQVRIGAGSKVGAHC
                     VVDGHTTIGRDNRFFPFASIGAAPQDKKYAGEPTRLEIGDGNTIREYVTINTGTVQDA
                     GVTRLGDDNWIMAYVHIAHDCRLGSHLILANAVQLAGHVHLGDWVFLGGLTGVHQFVR
                     VGDHAMTAFQTRLAQDVPPFVTAGGNPAEAQSINAEGLRRRGFSAERITLVKQMYRLL
                     YRKGLTLEAARQQIDALRGELAEADADIALMQDFLRTATRGIVR"
     gene            complement(15744..16187)
                     /gene="fabZ"
                     /locus_tag="PKN51_04520"
     CDS             complement(15744..16187)
                     /gene="fabZ"
                     /locus_tag="PKN51_04520"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNT84396.1"
                     /translation="MMDIHRILTKLPHRYPFLLVDRVLDIDLQARTIRALKNVTVNEP
                     FFTGHFPARPVMPGVLMLEAMAQACALLSFETEGISLGDDQVFYFVGIDGARFKRPVE
                     PGDQLILHAAIDRARAGIYRFNCKATVGDEVACEAAIMCTMRDVG"
     gene            complement(16247..17233)
                     /gene="lpxD"
                     /locus_tag="PKN51_04525"
     CDS             complement(16247..17233)
                     /gene="lpxD"
                     /locus_tag="PKN51_04525"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNT84397.1"
                     /translation="MTLRVGAIVQALGGELFGDPDRQITRLAPLEGAQPGELSFLVNP
                     RLTQLLAATRAGCLIVGPELAEAARARGDCIVAADPHLYFARLTQLWRRLHGGAARPG
                     IHPSAVVEEGAFVDPSASVGALSFVGRGARIGAGSVLKQRVTVGEDCVIGARCLIQSG
                     AVIGGDGFGFAPHEGAWVRIEQLGAVRIGDDVEVGANTCIDRGALDDTVIEDGVKLDN
                     LIQIGHNCHIGKHTAMAGCVGVAGSARIGAHCTVGGAGMILGHLTIADHVHISSASIV
                     TRSILKPGHYTGFFPLDDNEHWEKNAAALKQLSALRERIKRLERQQTPADDA"
     gene            complement(17295..17786)
                     /locus_tag="PKN51_04530"
     CDS             complement(17295..17786)
                     /locus_tag="PKN51_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNT84398.1"
                     /translation="MALALCGACFAAPVALAQEATRIGFVNTDRMLREAAPAKAAQSK
                     LEQEFSRREKELDDAGATLKAASERFERDASTMSESQRATRQRQLMEQDREFQRKRRE
                     FQEDLNARKQEELQQILDRANRVVKQVAEAEKYDVILQEAVYVHPRLDITDKVIKALN
                     GGK"
     gene            complement(17810..20107)
                     /gene="bamA"
                     /locus_tag="PKN51_04535"
     CDS             complement(17810..20107)
                     /gene="bamA"
                     /locus_tag="PKN51_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNT84399.1"
                     /translation="MTSFATRFRLRSAASLASLLLASHLAWAVEPFTVRDIRVEGLQR
                     VEPGTVFGSLPVRVGETYNDDKGAAAIRALFGLGLFNDVRLEVSGDVLVVIVQERPTV
                     AGVEFAGNREFSNEVLQGVMRDAGLTSGRPYDRALIDRAEQELKRQYINRSLYGAEVV
                     TTVTPTERNQVRLTFNITEGRPARIQNIDIVGNQAFSDGQLKDLFDLDTGGWLSWYTK
                     SDQYSRAKLNADLEALRSFYLARGYLDFKIDSTQVAIAPDKESIGITINVTEGERFVV
                     SGVTLDGNYLEKDDEFKSLVTIRAGQAYNADQVAETTKAFTEHFGNFGYAFARVEAVP
                     EVDRANNRVAIVIRSEPSRRAYVRRIHIQGNTRTRDEVIRREFRQYESSWYDAEKIKL
                     SRDRVDRLGYFTELSVTTAEVPGAPDQVDLNVEVKEKPTGSISIGAGYSSAEKLAFTF
                     GLSQDNAFGSGNKLDLQFNSGKYNRTVVVSATDPYFTTNGVSRTVDVYYRTQKPYQDQ
                     GGNYTLVTMGVGLKFGVPVTELDTIYLGLNAERTRIKPGTNIPAAYLDYANRFGYSSS
                     AFPFTLGWSRDSRDSALAPNSGRYQRLLGELSLFGDARYAKANYQYQQYIPLSKQYTL
                     AFNGEIGWGKGLGGRPFPVFKNYYAGGLGSVRGYDQSTLGPRDVTGAFIGGTRKFVLN
                     TEFITPFPGAGNDRTLRLFGFIDAGALYGEGEKIRGDALRYSTGVGLSWISPLGPLRF
                     AFAVPLRKRPGDRIQRFQFQIGTSF"
     gene            complement(20166..21542)
                     /gene="rseP"
                     /locus_tag="PKN51_04540"
     CDS             complement(20166..21542)
                     /gene="rseP"
                     /locus_tag="PKN51_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNT84400.1"
                     /translation="MLLTLIAFVVALGLLIAVHEWGHYRMAVARGVRVLRFSIGFGKT
                     IARFKPARQRPGQDTEFVIGAIPFGGYVKMLDEREAPVDEALRHQAFNTQPLASRALI
                     VAAGPVANLLLAVLLYALVNWIGVQEPRALLAPPVAGSLADKAGLQGGELVRQAAPAG
                     EALAPVASFESLRWVLTRAALDGQDLVLEVSRGADGRGRELTLPLSTLATRDPDAALF
                     RAIGIVSPLSSPTVGEVMPGGAAERAGLQQGDVVRRVGDVPVHDGQQLRELIRASGAS
                     GPPPASPWLIERDGRALTLQVRPDAHEDKGQPVGRIGAYVGSQPEMVLVRRGAADGLW
                     AGVVRVVEVSGLSLRLLGRMLVGELSLRNLSGPIAIADYAGKSASLGLTYYLAFLAFI
                     SVSLGVLNLLPLPVLDGGHLMYYLWEAVTGRPVGGVWMERLQYVGMSLLLAMMAIAMY
                     NDIVTRLG"
     gene            complement(21576..22769)
                     /locus_tag="PKN51_04545"
     CDS             complement(21576..22769)
                     /locus_tag="PKN51_04545"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNT84401.1"
                     /translation="MRRQRLAVLGSTGSIGTSTLDVVARHPERFEVVALTAATQVDAM
                     LAQCERFRPRVAVMASAPHAAALAQKIKQKGIPTQVEQAPEAIENIACWGEVDTVMAA
                     IVGAAGLAPCLAAARAGKRLLLANKEALVVGGEVFLRAVQAGGATLLPIDSEHSAIFQ
                     SLPEDPATWAARIDKIILTASGGPFRTRAPESLAAVTPEQACAHPNWVMGRKISVDSA
                     TMMNKALEVIEARYLFGVDPDQLQVVIHPQSVIHSMVQYKDRSVVAQLGTPDMRVPIA
                     YGLSWPERIESGAAALDFHALGGLSFEPIDADGHRERFPGLALAWEALRAAPGTCAVL
                     NAANEVAVDAFLQRRIRFDQIHAVNRATLDAEAPGAPGDLAALLELDHRARAAALACV
                     RRLAG"
     gene            complement(22766..23632)
                     /locus_tag="PKN51_04550"
     CDS             complement(22766..23632)
                     /locus_tag="PKN51_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNT84402.1"
                     /translation="MLGQRVITALVLLAVLLPALFAPSATPFIALSLLFIAAAAWEWA
                     RLNGVAGAGALATGALCAALCLGLWALGAVERPLPGLWLAAALAWVLGGAWVLGRGVA
                     GWARVPAALRWALGLALLSVAWLAMAQARRIGVNFLLSAMVLVWVADVFAYFAGRALG
                     GRLTGGNKLAPTISPGKSWEGVWGGMLGVLALAVLWLWLDRTQPPGAASIYTRLHQAG
                     PALLVAGVLFLCAMSVVGDLFESLVKRSAGMKDSSQLLPGHGGVLDRIDALLPTLPLA
                     MLLASWSAGGGA"
     gene            complement(23646..24377)
                     /gene="uppS"
                     /locus_tag="PKN51_04555"
     CDS             complement(23646..24377)
                     /gene="uppS"
                     /locus_tag="PKN51_04555"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNT84403.1"
                     /translation="MANASTSHELPRHIAIVMDGNGRWARRRLLPRIAGHRQGVDALR
                     RCIRACAERGVQVLTVFAFSSENWSRPQDEVSGLMELLGKALTREVPQLQRAGIRLHF
                     VGERQALSEGMRHALAQAEADTTGNAGMVLNVCFNYGGRWDIVQAAARLAERGAPITE
                     ATLAGAMALAHVPDPDLLIRTGGEQRISNFLLWQCAYSELYFTDCLWPDFDAAALDAA
                     LAAYAGRERRFGRTSEQLAQAGAAG"
     gene            complement(24513..25073)
                     /gene="frr"
                     /locus_tag="PKN51_04560"
     CDS             complement(24513..25073)
                     /gene="frr"
                     /locus_tag="PKN51_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNT84404.1"
                     /translation="MSIADIKKHTEGKMDQSIAAFKNNLTKIRTGRANPALLDTIHVE
                     YYGNHVPLSQVANVSLLDARTISVQPWEKGMGAKIEKAIRESDLGLNPASMGDLIRVP
                     MPPMSEERRREMTKIVRHEGEQAKIAIRNLRRDANDAIKKLVKDKEATEDEQKRSEAD
                     IQKVTDRHVADIDALVAAKEKDIMEV"
     gene            complement(25103..25825)
                     /gene="pyrH"
                     /locus_tag="PKN51_04565"
     CDS             complement(25103..25825)
                     /gene="pyrH"
                     /locus_tag="PKN51_04565"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNT84405.1"
                     /translation="MTKAKPAHRRILLKLSGEALMGDDAFGINRATIERIVGEIAEVT
                     RMGVQVAVVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMNALALADAMNKHGLTAR
                     VMSAIAIEQVVEPYVRPKALQYLEEGKVVVFAAGTGNPFFTTDTAAALRGAEIGAELV
                     LKATKVDGVYTADPNKDPKATRYARLSFDEAMSRNLGIMDATAFALCRDQKLPIKVFS
                     IFKPGALRSVVLGADEGTLVHV"
     gene            complement(25952..26845)
                     /gene="tsf"
                     /locus_tag="PKN51_04570"
     CDS             complement(25952..26845)
                     /gene="tsf"
                     /locus_tag="PKN51_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNT84406.1"
                     /translation="MAAITASMVAELRAKTDAPMMECKKALTEADGDMAKAEELLRVK
                     LGTKAGKAASRVTAEGVIAAHIAGTTGAMIEVNCETDFVTKNDMFIAMAQAAARLVAE
                     HNPADVTALGALAYEQDGYGPTLEDVRKGLIGKIGENMSFRRFVRKAGGAQLTSYLHG
                     TRIGVLVEFQGDEVAARDVAMHVAAMKPKALTAAEVPADLVETERSVAAAKAAESGKP
                     ADIAAKMVEGSVQKFLKEVSLFNQPFVKNDKQSVEQMLKEKATQVSGFTLYVVGEGIE
                     KKQDDFAAEVAAQVAAAQGKA"
     gene            complement(26975..27730)
                     /gene="rpsB"
                     /locus_tag="PKN51_04575"
     CDS             complement(26975..27730)
                     /gene="rpsB"
                     /locus_tag="PKN51_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNT84407.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPKMAPFIFGHRNKIHIINLEK
                     SLPMLQEAQKFARQLAANRGTILFVGTKRQAREIVAEEARRCGMPYVEQRWLGGMLTN
                     FKTVKTSIKRLKDMQAQQEAGLDSMSKKEQLMFTREMEKLERDIGGIQDMGALPDAIF
                     VIDVGYHKIAVSEAKKLGVPLIGVVDSNHNPDGIDYVIPGNDDSARAVTLYARALADA
                     VLEGKANAVNDVVKAVQAEGEDEFVEVEDDGAA"
BASE COUNT         4227 a         9501 c         9585 g         4507 t
ORIGIN      
        1 tgaagatgat gcagggcgcg ttcttcttgg cgttctcgaa catgtcgcgc acgcgcgccg
       61 cgcccacgcc gacgaacatt tcgacgaagt ccgagcccga gatgctgaag aacggcacct
      121 tggcctcgcc ggcgatggac ttggccagca gcgtcttgcc ggtgcccggc gggccgacca
      181 gcagcaggcc gcgcggaatg cggccgccca gcttctggaa cttctgcggg tccttgagga
      241 agtccaccac ttccttgacc tcttccttgg cctcgtcgca gccggcgacg tcggcaaagg
      301 tgatggtgtt gttgttttcg tccagcatgc gcgccttgct cttgccgaag ctgaaggcgc
      361 cgcccttgcc gccgccctgc atctggcgca tgaagtagat ccagacgccg atcagcagca
      421 gcatcgggcc ccacgacacc agcagtgtca tcagcagcga gccttcttcg cgcggcttga
      481 cgtcgaactt gacgttgtgg ttgatcagat cgcccaccag cccgcgatcc aggtaggtgg
      541 cctgggtgcg cacgcggcgg tcgtcgctca tcacggccac gatgtcggtg ccgccctgcc
      601 cttcctggat ggtggcctgc ttgacgcggt tgttgcgcac ttcctccagg aagtcggaat
      661 aggccatgtg gttggcgctg gccatgcggc cgccgtcgaa ctgtttgaag acagtgaaca
      721 gcaccatggc gatcaccatc cagatggcga tcttcgagaa ccattcgttt ttcaaggcct
      781 tgcgctccag tgggtcagtc cggcgatgcc agacaggtga aggccatttt aggcttttca
      841 acccatggcg ggcgcccgtg cgccggggta accgcaggga cgaaatcccc aatttttccc
      901 ggcaccgggg gcgcacagac gcctgccccg ccgcggcagg gccgaaattg atgcaaatca
      961 acccgtttcg gcgggcggct tgggtccgat gccgaccaga aaggtctcgg ccgagcggtc
     1021 gcgcgaggcc ttgggcttga ccggcttgac caccttgaag ctgtccttga acagcttgac
     1081 gagttggctg tagccgctgc cgtgaaacag cttcaccacc agcgcgccgc cgggcttgag
     1141 gtggcccagg gcaaagtcga gcgccagttc caccaagtgc gcgatgcgcg cggcgtcgct
     1201 cgactcgacg cccgacaggt tgggcgccat gtccgacacc accaggtcca gcggctgccc
     1261 acccaccgct tcattcaggc gcgccagcac ctcgtcgtcg cggaagtcgc cctgcagaaa
     1321 atgcacgccc tcgatcggct ccatgggcag gatgtcgagc gccacgatgg tgccgtccag
     1381 cgcgcccacc gccgcgcccg cgggcgccag gcgccggcgc aggtactggc tccaggcgcc
     1441 ggggctgctg cccagatcga ccacccgcat gccgggtcgg atcaggccca gcgtctcgtc
     1501 gatctccttg agcttgtagg cggcgcgggc gcggtaaccc tccttctgcg ccagcttgac
     1561 gtaggggtcg ttgatgtggt cggccagcca ggccttgttg accttcttgc ttttgctctt
     1621 gactttcatg taggcgtatt ctggccgcac cggcggcgtg gcagtccgcc ctggcgccgc
     1681 gaactcgggg cgcacgggcg ccggccgtgg acataatacg gcccatgccg cacatccaac
     1741 tcacccccgc cgaacgcaag gcgcaccgcg ccgaagcgca ccacctggac ccggtggtca
     1801 tggtcggcgc cgacggcctg acgcccgccg tggtgaagga aaccgacgcg gcgctcaacg
     1861 cccatggcct gatcaagctg cgcgtgctgg gcgacgaccg cgccgcgcgc gaggcgatgt
     1921 ttcagcaact ggccgatcag ctcaacgccg cgcccatcca gcacatcggc aagctgctgg
     1981 tgctgtggcg gcccaaaccg gccaagccgc gaagcctgga cgaagaacgc cggcccggcc
     2041 ccaaggacgt caaggtcatc aaacacagca agcgcggtgg ccagcggccc gaagtcaagg
     2101 tggtgcgcgt gctgggcaac cagcgcctga cgcccggcgg caaggtgaag cgggcgcagc
     2161 cgaagcagaa gtcggtcaag aaggggcgcg ccgactgacg gcgcatgctt cgattttcat
     2221 agctgcctgc gcatgtccga caaccgccag cgccatattc tgtgcatgaa atgggggacc
     2281 aagtatggcc ccgagtacgt caaccgcctg tacggcatgg tgcgtcgcca cctggcgggc
     2341 gagttccagt tcatctgcct gaccgacgac gccagcggtg tgcgccccga agtgcgctgc
     2401 ctgccgatcc cgccgctgaa cctgcagctc aagcccggcc agcgcgacgg cgcctggaag
     2461 aagctcacca ccttcgaggc cgacctgcac ggcctgaccg gcaccgccct gtttctggac
     2521 ctggacgtgg tggtggtcgg cccgctggac gacttcttca cgcagccggg cgagtttctg
     2581 atcatccacg actacccgcg cttctggcgc ttcggcgagc gcgtggtcgg caactcgtcg
     2641 gtctaccgct tcgcgctggg cgcccacgcc gacgtgctgg cgtactttcg cacccacatg
     2701 gacgaggtgc aaaaacaata ccgcaacgag caggactttc tgtcgcactt cctgcaccgg
     2761 cagggcaagc tgaactactg gccggcggcg tggtgcccca gcttcaagta ccacagcatc
     2821 ccgacctggc cgaccaacta ctggaaggcg cccatcgtgc cgccgggcgc gcgcatcgtg
     2881 atcttccacg gcgaagtcaa cccgccggac gcactggcgg gcaagcgcaa caagcgcttt
     2941 gccttcatca agccggccct gtgggtggcc gacgcctggc agggctgagg cgccgccggt
     3001 tgatgggtgt ttcagggctc cagcgcccgt ccagattgcg ccagcagctc tttaaacgat
     3061 agcagtccgg tcagcgcggc gtgcccgcgc cgacaccgac cagcggccgc ccgaccagcc
     3121 ttgagaggat cgccagcggg ctggcctggg ggttggcctc gcgccccggc ggcaggtgca
     3181 gggtctgctc catcatgaac tgcccgctca tcaccgcgtg tgtggcctgg tccgaaaagc
     3241 acacccaggc gctgccggcg gcaaacggca tgctgacctg gccaccgctt tgctggtagc
     3301 gctcgtcccg cttcatcagg tcgtgcagtt gcagcatcag gtggtcgtac tcgctgcgca
     3361 gcgacttggt gacgtgcagg gcgttgaggg cccgcgcctg ccacaggcga tagggcttgg
     3421 cgcgcggcag gaactgccgg gccacggtct cgaagtcctc gcccacgcgc cacacgcgcg
     3481 gcacgccctg cgggttgatg ttggcaaaca cgcgcaggat gcgttcgcca tagttgggac
     3541 gtgagggaaa ggcatcgacg tgcaggcgct ggtcgtcggc gcgaatggac tgcgcgcgcg
     3601 tctccacctg gcgcggccga aagctggtgg gcgccacacg cagcaggccc cggtacgccg
     3661 gcagcaggcc atcgaccagc tgcagcgcct ggccgcgaaa gcgcccgatc atggccgcca
     3721 gcgcccgctg ttcgtcggcg ctgccgcccg cgcccttgag cacgccatcg gcgcccaggc
     3781 tgacgttgcg cgtgccggcc gccagcatgt cttcgcgcag cagggccagc tcgtgccctt
     3841 cgagctgaaa cctcagctgc ggaaagtaca gcaccttgcc cgcctcgacg gcggccgtcc
     3901 actccggccg gctgctcgcc tggccccagt cgttcagcgt cagctcgacg atcggcgcgt
     3961 ccatcccctc acctccgtgg tgtaccgccc aggcgccaca gcaccacgcc ggcgcacagc
     4021 cactgcaggg cgaacatggc cgtgcccagc ccgtgccaca gcggcaggtt gacgcgcgcc
     4081 acgatgcgcg gggcgacggc aaattcgatc agcagcgcca gcagcatgcc ggccatcacc
     4141 aagcccagcg tggtgcgcgc ccgcacctcg cccgcagcgc cggcgcggcc acgcagcagc
     4201 agcaacagca aggcgccaca caccagcgac acccaggcct gggcggtgaa caccaccgcc
     4261 gccgtctggc ccgccagcgc cttggacggc agcagcctga acagcaacgg caccacgaag
     4321 ccgatcacgg ccgacaaggt gccccaccac agcgcggcag ccagcagggc cagacggtcg
     4381 gacatggggc cgcccctcag acgtagcgca cggcgatcac ttcgtagtgc cgcacgccac
     4441 ccggcgcctg cacttcggcg gtgtcgccct cttccttgcc gatcagcgcg cgcgcgatcg
     4501 ggctgccgac gttgatcagg ccgtgcttca ggtcggcctc gtcctcgccg acgatctggt
     4561 aggtgacctg ctcgccgctg tcctcgtcct ccagctccac cgtggcaccg aagacgatgc
     4621 gcccgccggc gtccagcccg gccgggtcga tcacctgggc cgcggccagc ttgccctcga
     4681 cctccttgat gcgaccttcg atgaatccct ggcggtcctt ggccgcctcg tactcggcgt
     4741 tctcgctcag atcgccctgg gcgcgcgcct cggcgatggc attgatgacc tcgggacggt
     4801 ccttggtctt caggcggtgc agctcggcct tgagcatctc ggcgccgcgc ttggtgatcg
     4861 gaaaggtggc catgaaaaaa tagaatgaag aaacgtgcgg atgagcagaa aacacgaaac
     4921 catctgcgcc gggcacagat ggtctgaaca gcgcacgatt ttaggccaac ggcgcgccct
     4981 gccctgcgcc accggccacc tgcaacgaaa cagccacgcc atgatccagc cgacccgccg
     5041 ccacctgctg cacgccagcg ccgccatggc caccaccgcc ctcgccggcc gcgcgctggc
     5101 gcaggacagc tgcgccgtac ccacgcgcga cgtgcaggct gccatcagcc ccgacgccgc
     5161 gctgcagcgc ctgcgcgaag gcaacgcccg cttcgtcgcc ggccagccca tccagtgcga
     5221 cctgcgagcg caggtgcgcg ccagcgccgg cggccaggcg ccgtttgccg ccgtgctggg
     5281 ctgcatcgac agccgcgtgc cgcccgaatt ggtgttcgac cagcgcctgg gcgacatctt
     5341 tgccgcccgc gtggccggca acttcgtcaa cgacgacatc ctgggcagcc tggaatttgc
     5401 cacccagctg gccggcgcgc gcctgatcgt ggtgctgggc cacaccgagt gcggcgccat
     5461 caagggcgcg gtggacaacg caaaactcgg ccacctgacc ggcctgctgg cccagatccg
     5521 cccctcgctc gaacggctgg actaccgcgg cacgcccagc tccaaggaca aggcgctggt
     5581 gcagcgcgtg gccgagcgca acgtgcagga cgccgtgacc aggctgaacg ccgcgccggt
     5641 gctggcggcg ctggtgaagg cggggcagct gcgcatcgtg ggggcgatgc acgatgtggc
     5701 gacggggcgg atcggctggc aggtttgaag ttgggcgagg cccgacgttt cactccaacg
     5761 acgaaagcaa cccaagccaa cccgccaccg ggtgccgaga tcagttggcc tttacgccag
     5821 agatcctgac cagcttctgg atcaccggat caaccgcatc gaagtccttg tggaaccgcg
     5881 ccgcgccacc aggcacggac tccaggccga agacctgaaa acgcgccttc atggccgtgg
     5941 agtgaatgat ttccaccgtt tccttctcca ggcgctgcaa gatcggcttg ggtgtgcccg
     6001 cgggtgccag cagcgtgaac caggcgacgg tgttgaactc ggggtcggca aagccctgct
     6061 cggccatggt ggccacatcg ggcagttcgg ccaggcgctt ggggccatag atggcgatgg
     6121 ggcgaatgcg cttgtccttg agcgcaccct gggccgcagc aatggtgccc aggccccagg
     6181 agaccacgcc gccggccagg tcctgaatgt gcggcgcctc gctcttgtag gccacatggg
     6241 tcatgtccag cttgcgcgag tcgctcaggt aggcgctcat cagatggcct ggcgagccct
     6301 ggccatagga gccgtagttg accttgccct tgttctgctg tgcccaggcc atgaattcct
     6361 tcatgttctt cgccggcaca tcggcgttga ccatcatcac caggctggtg gccgcaagct
     6421 gggagatgaa ctcgaaatcc ttgcggatgt catacggcac cttggcaaaa atgtggtgag
     6481 cgtaaaagat gggggtggaa taggccacca gcagcgtgtg gccgtcgggc gccgcgcgcg
     6541 ccacggcctg ggtgcccagc atgcccgagg cgcccgtctt gttgtccacc accacggcct
     6601 ggcccaggcg cttgcccagc tcatcggcca gcacgcgcgc caccacgtcg gtaccgccgc
     6661 cggcgccggc cggcacgatc aaggtgacgg gccgattcgg gtagttggcc gtctgcgccg
     6721 acgccatggc cgcgaggccc agggccaaga aggccagtac ggatttgatg cccatgcttt
     6781 cctcccaaaa caaagggagg gcatgatgcc cgccggtctg gccgcgatcc atcggaagat
     6841 acccgcaggc cgcgcgccgc cagatggccc tggcagaggg gcggatgcac cggtggggtc
     6901 gcgcgtcagg gtggatcggg tgtcactccg gcgtcactgc gaccccgcct atcgctcagg
     6961 ccaactgcgc atgcatctcc tgcaccgaaa tcacgctcag ctgatccatg cacttcatgc
     7021 cttcgacggc cgcttcggcg ccgaagatgg tggtgatggt gggcacacgc gcggccaggg
     7081 cgctggtgcg gatggcgcgg ctgtcggtga tggcgttgcg gcgctcttcg acggtgttga
     7141 tgaccagcga gatgtcgtcg ttcttgacca tgtcgacgat gtgcgggcgg ccttcggtga
     7201 ccttgttgac ggtctcgcag gcgatgccgg cgtcgcggat ggcctgcgcc gtgccgcgcg
     7261 tggccaccag gccgaagccc atgccggcca gctcgcgcgc gatctgcacc gcctgcggct
     7321 tgtcgctgtt cttgaccgtc aggaacacct tgccggccgg cgtgccgtcg gcgcgcagcg
     7381 ggcgcggcag gcgctcgccg gcgcccagct gggccttgat gtaggcctcg ccaaaggtct
     7441 tgccgacacc catcacctca ccggtggact tcatctcggg gcccaggatg gtgtccacgc
     7501 cggggaactt gacgaacggg aacaccgctt ccttgacgct gaagtacggc ggcgtgacct
     7561 cggcgccaat gccctgcgcg tccagtgtct ggccggccat gcagcgcgcc gccaccttgg
     7621 ccagctgcac gccggtggcc ttgctgacga agggcacggt gcggctggcg cgcgggttga
     7681 cctcgagcac gtagatcacg tcctgcttgc cgccgccttc cagcggtttt tcctggatgg
     7741 cgaactgaac gttcatcagg ccgaccacgt tcaggccctg cgccatggcg gcggtctggc
     7801 gcttgagctc ggccacggtg gcggcgctca ggtagtacgg cggcagcgag caggccgagt
     7861 cgcccgagtg cacgccggcc tgctcgatgt gctccatcac gccgccgatg aacacgcgcc
     7921 cggtgctgtc gcgcacggcg tcgacgtcgc actcgatggc gtcggacaag aagcggtcca
     7981 gcagcacggg tgagtcgttg ctgaccttca cggcctcgcg catgtagcgt tccaggtcgc
     8041 gctgttcgtg cacgatttcc atggcgcgcc cgcccagcac gtagctgggg cgcaccacca
     8101 gcgggtagcc gagctcgctg gccttggcca gcgcctcggc ttcggtacgg gcggtggcgt
     8161 tgggcggctg gcgcaggttc agctcgtgca gcagttgctg gaagcgctcg cggtcttcgg
     8221 cggcgtcgat catgtcgggc gtggtgccga tgatgggcac gccctcggct tccaggccca
     8281 gcgccagctt gagcggcgtc tggccaccgt actgcacgat cacgcccagg ggcttttcct
     8341 tgtcgacgat ttcgagcacg tcttccagcg tgagtggctc gaagtacagg cggtcgctgg
     8401 tgtcgtagtc ggtggagacg gtctcggggt tgcagttgac catgatggtc tcgtagccgt
     8461 cttcgcgcat ggcgagggcc gcgtgcacgc agcagtagtc gaactcgatg ccctggccga
     8521 tgcggttggg cccaccgccc agcaccatga tcttcttcct gtcggtgggt tcggcctcgc
     8581 actcgtcctc gtaggtggag tacaggtagg cggtgtgggt ggcgaactcg gcggcgcagg
     8641 tgtcgacgcg cttgtacacc gggcgcacgc ccagggcgcg gcgcgcgtca cgcaccgcct
     8701 tgtcgctggt cttcagcagc ttggccaggc ggcggtcgga aaagcctttc tgcttcagcg
     8761 ccagcagctc tgctttcgat agcgcggcga gcgcccggtc gccctgctgc tcggtgtgac
     8821 ggtcgagctc cagctcgatc ttgacgatct cctcgatctg caccaggaac catttgtcga
     8881 ttctggtgag ctggtgcact tcatccagcg accagccggc ggcgaaggcg tcgccgacga
     8941 accagatgcg gtcgggcccc ggctcgccga gctccttttc gagccattcg cggtcctgcg
     9001 tcttctcgtt catgccgtcg acgccgactt ccagcccgcg cagcgccttc tggaacgatt
     9061 cctggaaggt gcggccgatg gccatgacct cgcccacgct cttcatctgc gtggtcaggt
     9121 ggctgtcggc ggccgggaac ttctcgaacg cgaagcgcgg gatcttggtg accacgtagt
     9181 cgatggtcgg ctcgaagctg gccggcgtgg cgccgccggt gatgtcgttc ttcagctcgt
     9241 cgagcgtgta gcccaccgcc agcttggcgg ccaccttggc gatcgggaaa ccggtggcct
     9301 tggacgccag cgccgacgag cgcgacacgc gcgggttcat ctcgatgatg atcatgcgcc
     9361 cgtcggccgg gttgacggcg aattgcacgt tggagccgcc ggtgtcgacg ccgatctcgc
     9421 gcagcaccgc caggctggcg ttgcgcatca gctggtattc cttgtcggtc agcgtctgcg
     9481 ccggtgccac ggtgatcgag tcgccggtgt gcacgcccat cgggtcgagg ttctcgatcg
     9541 agcagatgat gatgcagttg tccgccttgt cgcggatcac ctccatctcg aactccttcc
     9601 agccgagcag ggattcttcg atcagcagct cgttggtggg cgaggcttcc aggccgcgct
     9661 tgcagatggt ttcgaactct tccgggttgt aggccacgcc gccgccggtg ccgcccaggg
     9721 tgaagctggg gcggatgacg gtcggaaagc cgacgcgctt ttgcaccgcc caggcctcgt
     9781 ccatgctgtg ggcaatcccc gagcgcgccg actccaggcc gatgcgcgtc atggcgtcct
     9841 tgaacttcag gcggtcctcg gccttgtcga tggcctcggg gcgcgcgcca atcagctcga
     9901 cgttgtactt ggccagcacg ccgtggcgcc acaggtcgag cgcgcagttg agcgcggtct
     9961 gtccgcccat ggtgggcagg atggcgtcgg ggcgctcctt ggcgatgatc ttctccaccg
    10021 tctgccaggt gatgggctcg atgtaggtgg cgtcggccgt ggccgggtcg gtcatgatcg
    10081 tcgcggggtt gctgttgatc aggatgacgc ggtagccctc ctcgcgcagc gccttgcagg
    10141 cctgcacgcc ggagtagtcg aactcgcagg cctggccgat gacgatgggg ccggcgccga
    10201 tgatgaggat ggattggata tcggtgcgtt taggcatggc ggtcgtggtt cagcttgtcg
    10261 agcagggcct gggcgcgctg cgggggcatg ttcttctgca tcagttggat caggccgtcg
    10321 ccctcgatca gcgggcgcag cacttcggcc tcggcgtcgt agaagcggac gtcccaggcg
    10381 gccagttcgc ggtcccactc ctcgtcggtc caggtcttgc ccttggccag cagggtgacc
    10441 agcggcatgg ccttgtgctc caggaaggac ttcttgtagg gcttttgcga ccagcgatcg
    10501 gcaaaccatt cgcggtgcgg cgacgccagg cccagcatgc ggcgggcgat ggccttttcc
    10561 agggtggcct gcgggaaggc gtacagcttc atgccgtggc cactcctgtt tgaatagcgt
    10621 ttggcgcagt accggcgccg gtttgctcca tgttttgaat gaaacgatcg aacaggtagc
    10681 cgacgtcgtg cggcccgggc agcgcctccg gatggccctg gaagcagaac gccggctggt
    10741 ccgtgaagct caggccctgc aggctgccgt cgaacaggct gacgtgggtg gggcgcaggt
    10801 tggccggcag ggtggcggcg tcgaccgcga agccgtggtt ctggctggtg atcgacaccc
    10861 ggccgtcgtc caggtccttg accgggtggt tggcgccgtg gtggccgaat ttcatcttgt
    10921 aggtgcgcgc gccgctggcc agggccatga tctggtgccc caggcagatg ccgaacaccg
    10981 gcttgccgct gtcgatcagg gtgcgcgcgg cggcgatggc gtagtcgcag ggctcggggt
    11041 cgcccgggcc gttggagaga aacacgccgt cgggctgaag cgccagcacc tgggcggccg
    11101 gcgtctgcgc cggcaccacg gtgacgcggc agccgcgcgc ggccagcatg cgcaggatgt
    11161 tcttcttgac gccgaagtcg taggcgacga cgtgaaagcg cggcgcctgc tgggtgccga
    11221 agccggcgcc caggtgccat tcggtctggt tccactcgta ggcacggtcg gtgctgacca
    11281 cccgcgccag gtccagcccg gtcatggcgg gcgcctcacg cgcggcggcg acggtggcgg
    11341 cgacgcgctc gggcgtcacc gcctcgcccg ccgccaggcc gacgatggcg ccattttgcg
    11401 ccccatgcgt gcgcagcagg cgcgtgagct tgcgggtgtc gatgccggcg atggccaccg
    11461 tgccttcggc gcgcaggtac tcgctcagcg agcgttcggc gcggaagttg ctgaccagca
    11521 gcggcaggtc cttgatgacc aggccggcgg cctgcacgcg ggcggattcg acgtcttccg
    11581 cgttgacgcc cacgttgccg atgtgcgggt aggtcagggt gacgatctgg cggcagtagc
    11641 tggggtcggt caggatttcc tggtagccgg tcatggcggt gttgaacacc acttcaccga
    11701 cgctggtgcc gctggcaccg atggattgac cgatgaagac gctgccgtcg gcgagggcca
    11761 ggatggcggg tgggaatctt ccctggagag acgaaagcac ggggttctcc gatggatgtc
    11821 ggtcgcccgg gcgcgcggct gggacgcagg gcgcgtcgca acggcttctc tgagagggga
    11881 tggactgctt tggtgcgctc gggcgacgcg gtttcgagca aagccactca ttatattccg
    11941 cccccctgat ccgaccgctg gcgcggggtt ttggccggcg gctggcgtca gggccgcgcc
    12001 accgcgctgg cgtgcaggca gatggccgcc gccgccgcca cacccagcga ttcttccccg
    12061 ccgggctggg caatgcgcag tgccagcgcg gcgcgttcga ccagcgccgg ctgcacgccc
    12121 tgcccttcgt gccccagcac ccaggcgcag ggccagggca gcgacgcacg gtgcagccac
    12181 tggccctggt gcgagctggt caccagcagc ggcacgcgca gtgcggccag ctcgggcacc
    12241 gtcagcgcat cgaccagccg caggccgaaa tgcgccccca tgccggcgcg cagcactttg
    12301 ggcgaccaca gcccggccgt gccgctgagc gccgccacct gggtgaagcc aaacgccgca
    12361 gcactgcgca ggatggagcc gacgttgccg gcgtcctgca cgcggtcgag caccacggtg
    12421 ggggcgtcgg gcgccagcgc cggcgccgct ggcagaggca gcacgaaggc catcggcggc
    12481 ggcgattcca gcgcgctcag ggcgcccatc aggccatctg caaacactac gtttttaata
    12541 gctgcttgcg caagctgggc gggcgccaga ggccaaaatg attcagaaaa cacggccagc
    12601 tcagggcgcc agccgcgggc caaggcggcg cgacacaggt ggtcgccttc cacccacaga
    12661 cggccctgcc ggcgccaggc gccgctgtcc tgcgccagcc gccgcaggtc cttgaacagt
    12721 gcgttgtcgc gcgactggac gcgcagcggc tcggccggcc tcatgcctcg caccccgcgc
    12781 gggcgatggc cgccgccacc ggggcaaacg agcgccgatg ccacggacac gccccatgcg
    12841 cgcgcagcgc cgccagatgc gccgccgtgc cgtagccctt gtgggcggca aaaccgtatt
    12901 gcggccaggc ggcatcgacc tcggcgcacc agcggtcgcg ctgcaccttg gccaggatcg
    12961 aggcggccga aatcgccggc acccgcgcgt caccgcccac caccggttcg gcgcgcacgg
    13021 ccagctgggg caggcggttg ccgtcgatca gcaccttggc cggcggcagg cgcaggccgg
    13081 ccacggcgcg ctgcatggcc agcagcgtgg cctgcaggat gttgaggcgg tcgatttcct
    13141 ccaccgaggc ttcggcgacg gcgcaacaca gcgccttggc gcggatttcg tcgtacagcc
    13201 gctcgcgccg gcgcgccgtg agcttcttgg aatcggccag accggcgatc ggctggcgcg
    13261 ggtccaggat gaccgccgcc gccaccaccg ggccggccag cggcccgcgg ccggcctcgt
    13321 ccacaccggc cagcaggccg ggtgtgtccc agtcgagcgc ggcttgttca gcgcgcaaaa
    13381 agtgcttcga tcgcatcggt ggccaggcgc gcggtgtcgc gccgcaagtc gtggtgcaag
    13441 gcggcaaaac gcgcctcggt ggcggcgatt ttctcaggcg cggccagcca gtccagcacc
    13501 gcctgcgcca gcgcggccgg cgtggcggcg tgctgcagca gctcgggcac cacgaacggc
    13561 gcgtccggac ggtcggccgc ctgctgcgcc gtcaggccgg cgggccggcg cagccattcg
    13621 ggcggcgcgc acaggatgtt gggcaggccc acccagggca gttggcgcct ggcgcgcatc
    13681 agccggtagc tgagcgccgg catggcgtag gcgatcacca tcgggcgctt gaacagcgcc
    13741 gcctccagcg tggcggtgcc gctggcgatc agcgtcacgt cgcaggccgc cagcgccgcg
    13801 tgcgactggc cgcgcagcac cgcgagcgac gcacccagac cgctggcggc ggccagcgcc
    13861 tgcacctggg gcagccgctc gggcaccgtc ggcactatga attgtgtagc tggtcgcgct
    13921 tgccggatgc gcgctgcagc ctgaaaaaac ctggaagcca gatgcttgat ctcggccgcg
    13981 cggctgcccg gcagcagcgc caccacgggc gattgcgcat ccagccccag ggcgcggcgc
    14041 gccgcggcgc ggtcgctctg cagcgggatg gcgctggcca gcgggtggcc gacgtagctg
    14101 gcggcaatgc cctgctcggc cagcaacgcc ggctcgaacg ggaacagaca gaggacgtgg
    14161 tcggcggcgg ctttcagctt gagcagcttc tcgggccgcc aggcccagaa cgaggggctg
    14221 acgaagtgca gggtgcggat gccgcccgcc ttcagccgtg cttcgaggtc gaagttgaag
    14281 tcgggcgcat cgacgccgat gaacagatcg ggccgctcgg ccagcagccg atcgcccagc
    14341 tggcggcgca ggcgcagcag cgcgggcagg cgcggcagca cttccaggta gccgcgcacc
    14401 gccagccggt cgaccggcca ccaggcctga aagccgcgcg ccgccatctc ggcgccgccc
    14461 acgcccatcg cctgcaggcc gggccagcgc gcacgcagcg cgtccagcaa atggccggcc
    14521 agcaggtcgc ccgaggcttc gccggcgacc agggcaaatc ggggggcggc ggcgacgcga
    14581 accggcgcac ccatggcgtc agcgcacgat gccgcgcgtg gccgtgcgca ggaagtcctg
    14641 catcagcgcg atgtcggcat cggcctcggc cagctcgccg cgcagggcgt cgatctgctg
    14701 gcgcgccgct tccagcgtca ggcccttgcg gtagagcagg cggtacatct gcttgacgag
    14761 cgtgatgcgc tcggccgaaa agccgcgccg gcgcaggccc tcggcgttga tgctctgcgc
    14821 ctcggccggg ttgccgccgg cggtgacgaa gggcggcacg tcctgcgcca gccgggtctg
    14881 gaaggccgtc atggcgtggt ctccgacgcg cacgaactgg tgcacgccgg tcagcccacc
    14941 caggaacacc cagtcgccca gatgcacatg gccggccagc tgcaccgcgt tggccaggat
    15001 gaggtggctg cccagccggc aatcgtgcgc gatgtgcacg taggccatga tccagttgtc
    15061 gtcgcccagg cgcgtgacgc cggcgtcctg cacggtgccg gtgttgatgg tgacgtactc
    15121 gcggatggtg ttgccgtcgc cgatctccag ccgcgtgggc tcgccggcgt acttcttgtc
    15181 ctgcggcgcg gcgccgatgc tggcgaacgg gaagaagcgg ttgtcccgcc cgatggtggt
    15241 gtggccgtcg accacgcagt gcgcgccgac cttgctgccg gcgccgatgc gcacctgcgg
    15301 gccgatcacg gcgtagggac cgacctcgac cgtggggtcg agctcggcgc gcggatcgac
    15361 cagcgcagtt ggatggatct gtggcatttc aacctagaaa ccgcagttgg atgcgcctgc
    15421 cagtgccgtg atcggtatgc caggcaaggc gcgacgacgc ggcgggctgg tgcccgcaag
    15481 aaggagcaac gccgcatggc gcgccgagtg cggtgctggc gggtgcatag cgccttcacc
    15541 tggaaggtga aggctccgaa gcggcgcaag ctcagagttc atgcggcatt cccaagggtt
    15601 gcgagcggtc aactgcggtt tctaggttca atggtccgac ggccgcagag caggccatgc
    15661 cagcgaacgc cgtggccgga cctgcgccgg ccactggcgt tgcccccccc gcggggggaa
    15721 ggcgcgcagc gactcagggg ggttcaaccc acgtcccgca tggtgcacat gatggcggcc
    15781 tcgcaggcca cctcatcgcc gaccgtggcc ttgcagttga agcggtagat gccagcgcgc
    15841 gcgcggtcga tggccgcgtg caggatcagc tggtcgccgg gctcgaccgg gcgcttgaag
    15901 cgcgcgccgt cgatgccgac gaaatagaac acctggtcgt cgcccagcga tatgccctcg
    15961 gtctcgaacg acagcagcgc gcaggcctgg gccatggcct ccagcatcag cacgcccggc
    16021 atcaccgggc gggccggaaa gtggccggtg aaaaagggtt cgttgacggt gacgttcttg
    16081 agcgcgcgga tggtgcgcgc ctgcaggtcg atgtcgagca cgcggtcaac cagcaggaag
    16141 gggtagcggt gcggcagctt ggtcaggatg cgatgaatgt ccatcatggc gttgcttcta
    16201 tttcgatagc tggttgcgcc cgatcaacgg gcgcgcgtgg cccattttat gcgtcatcgg
    16261 cgggtgtctg ctggcgttcc agccgcttga tgcgctcgcg cagcgcgctg agctgcttga
    16321 gcgcggcggc gttcttttcc cagtgttcgt tgtcgtccag cggaaagaag ccggtgtagt
    16381 gcccgggctt gaggatggag cgcgtgacga tcgaggccga tgagatgtgc acgtggtcgg
    16441 caatggtcag gtggcccagg atcatgccgg cgccgcccac cgtgcagtgc gcaccgatgc
    16501 gcgcgctgcc ggccacgccc acgcagccgg ccatggcggt gtgcttgccg atgtggcagt
    16561 tgtggccgat ctggatcagg ttgtcgagct tgacgccgtc ttcgatcacg gtgtcgtcga
    16621 gcgcgccgcg gtcgatgcag gtgttggcgc cgacttccac gtcgtcgccg atgcgcaccg
    16681 cgccgagctg ttcgatgcgc acccaggccc cttcgtgcgg ggcgaagccg aagccgtcgc
    16741 caccgatcac cgccccgctc tggatcaggc agcgggcgcc gatcacgcag tcctcaccca
    16801 cggtgacgcg ctgcttgagc acgctgccgg cgccgatgcg ggcgccgcgg ccgacgaagc
    16861 tgagtgcccc cacgctggcg ctggggtcga cgaaggcgcc ctcttccacc accgcgctcg
    16921 ggtgaatgcc gggccgcgcc gcgccgccgt gcaggcgccg ccacagttgc gtcaggcggg
    16981 cgaagtacag atgcggatcg gccgccacga tgcagtcgcc ccgggcgcgc gccgcctcgg
    17041 ccagttcggg gccgacgatc aggcagccgg cgcgggtggc ggccagcaac tgggtcaggc
    17101 gcgggttgac cagaaaactc agttcgccgg gctgcgcgcc ttccagcggc gccaggcgcg
    17161 tgatctggcg atccgggtcg ccgaacagtt cgccgcccag ggcctggacg atggctccga
    17221 cacgcagggt catggccgca cgctcatgga caggacagcc accacggtcc ggggcgcggc
    17281 acgccaggcc ccggtcactt gccgccgttc agcgccttga tcaccttgtc ggtgatgtcg
    17341 agccgcggat ggacatacac cgcctcctgc aggatgacgt cgtacttctc ggcctcggcc
    17401 acctgcttga cgacacggtt ggcgcggtcg aggatctgct gcagctcttc ctgcttgcgt
    17461 gcgttcaggt cttcctggaa ctcgcggcgc ttgcgctgga actcgcggtc ctgctccatc
    17521 agctggcgct ggcgcgtggc gcgctggctc tcgctcatcg tcgaggcgtc gcgctcgaag
    17581 cgctcggacg ccgccttgag cgtggcaccg gcgtcgtcga gctccttctc gcggcgcgag
    17641 aactcctgct ccagcttgga ctgcgcggcc ttggcgggtg cggcttcgcg cagcatgcgg
    17701 tcggtgttga caaagccgat gcgcgtggct tcctgggcca gcgcgaccgg cgcggcgaag
    17761 caggcgccac acagcgccag ggccatcaga cgacgagaca agtacggctt caaaacgagg
    17821 ttccgatctg gaattggaag cgttgaattc tatcgcccgg cctcttgcgc agaggcacgg
    17881 cgaaggcaaa gcgcagcggg ccgaggggag aaatccagct caggccgacg ccggtggaat
    17941 agcgcagcgc gtcgccacgg atcttctcgc cttcgccgta cagcgcgccg gcgtcgatga
    18001 agccgaacag gcgcagcgtg cggtcgttgc cggcgcccgg gaagggggtg atgaactcgg
    18061 tgttcagcac gaacttgcgc gtaccgccga tgaaggctcc ggtgacgtcg cgcgggccca
    18121 gggtggactg gtcgtagcca cgcaccgagc ccaggccgcc ggcgtagtaa ttcttgaaca
    18181 ccgggaacgg ccgccccccc agacccttgc cccagccgat ttcgccgttg aaggccagcg
    18241 tgtactgctt gctgagcgga atgtattgct ggtactggta gttggccttg gcgtagcgcg
    18301 catcgccgaa caggctgagt tcgcccagca gacgctggta gcggcccgag ttgggcgcca
    18361 gcgcgctgtc gcgcgaatcg cgcgaccatc ccagcgtgaa cggaaaggcc gagctcgaat
    18421 agccgaagcg gttggcgtag tcgaggtagg cggcaggaat gttggtgccg ggcttgatgc
    18481 gcgtgcgttc ggcgttcaga cccagataga tggtgtcgag ctcggtcacc ggcacgccga
    18541 acttcaggcc cacgcccatg gtcaccaggg tgtagttgcc gccctggtcc tggtacggct
    18601 tctgggtgcg gtagtagacg tcgaccgtgc gcgagacgcc gttggtggtg aagtagggat
    18661 cggtggcgct gaccaccacg gtgcggttgt acttgccgct gttgaattgc aggtccagct
    18721 tgttgccgga gccgaaggcg ttgtcctgtg acagcccgaa ggtgaaggcc agcttttcag
    18781 ccgacgagta accggcgccg atgctgatgc tgccggtagg cttttccttg acctcgacgt
    18841 tgaggtcgac ctggtcgggc gcgcccggca cctcggcggt ggtgaccgac agttcggtga
    18901 agtagcccag tcggtccacg cgatcgcgcg acagcttgat cttctcggcg tcgtaccaac
    18961 tggattcgta ctggcggaat tcgcgccgga tcacctcgtc gcgcgtgcgc gtgttgccct
    19021 ggatgtggat gcgccgcacg taggcgcggc gcgagggctc ggagcggatc acgatggcca
    19081 cgcggttgtt ggcgcgatcg acttcgggca ccgcctccac gcgggcaaag gcgtagccga
    19141 agttgccgaa atgctcggtg aaggccttgg tggtttcggc cacctggtcg gcgttgtagg
    19201 cctggcccgc tcggatggtg accagcgact tgaactcgtc gtccttttcc aggtagttgc
    19261 cgtccagcgt cacgcccgac accacgaagc gctcgccctc ggtgacgttg atggtgatgc
    19321 cgatggattc cttgtccggc gcaatggcga cctgggtgga gtcgatcttg aagtcgaggt
    19381 agccgcgcgc caggtagaac gagcgcagcg cctccaggtc ggcattgagc ttggcgcgcg
    19441 agtactggtc gctcttggtg taccacgaca gccagccgcc ggtgtcgagg tcgaacaggt
    19501 ccttgagttg cccgtccgaa aacgcctggt tgccgacgat gtcgatgttc tggatgcgcg
    19561 ccggccggcc ttcggtgatg ttgaaggtca ggcgcacctg gttgcgctcg gtcggcgtga
    19621 cggtggtcac cacctcggcg ccgtagaggc tgcggttgat gtactggcgc ttgagctcct
    19681 gctcggcgcg gtcgatcagg gcgcggtcat acggccgccc ggaagtcagg ccggcgtcgc
    19741 gcatcacgcc ctgcagcacc tcgttgctga actcgcggtt accggcgaat tccaccccgg
    19801 ccaccgtggg acgttcctgc acgatcacca ccagcacgtc gccgctgact tccagccgca
    19861 cgtcgttgaa caggcccagg ccgaacagcg cgcggatcgc cgctgcgccc ttgtcgtcgt
    19921 tgtaggtttc tccgacgcgc accggcaacg agccgaacac ggtgccgggc tcgacgcgct
    19981 gcagcccctc gacgcggatg tcgcgcacgg tgaacggctc gacggcccag gccagatgcg
    20041 aggccagcag cagactggcc agcgaggccg cggagcgcag ccggaaacgt gttgcgaagg
    20101 atgtcatgaa aggctacggg aaacgatggc cgcgcgagac cggggaagag ctgtcggtga
    20161 aggggtcaac ccaggcgggt gacgatgtcg ttgtacatcg cgatggccat catggccagc
    20221 agcagcgaca tgccgacgta ctgcaggcgc tccatccaga cgccacccac gggccggccc
    20281 gtcacggcct cccaaagata atacatcagg tgccccccat cgaggaccgg cagcggcagc
    20341 aggttgagca cgcccaggct gacgctgatg aaggccagaa aggccaggta gtaggtcagc
    20401 cccaggctgg ccgacttgcc cgcatagtcg gcgatggcga tcggtccgct caggttgcgc
    20461 agcgacagct cgcccaccag catgcgcccc agcagccgca gcgacaggcc ggacacttcc
    20521 accacccgca ccaccccggc ccacaggccg tctgcggcac cgcggcgcac cagcaccatc
    20581 tcgggctggc tgccgacgta ggcgccgatg cgacccaccg gctgaccctt gtcctcatgc
    20641 gcgtccgggc gcacctgcag cgtcagcgcg cggccgtcgc gctcgatcag ccagggcgac
    20701 gccggcggcg gcccgctggc gcccgaggcg cggatcagct cgcgcagctg ctgcccgtcg
    20761 tgcaccggca cgtcgcccac ccgacgcacc acgtcaccct gctgcaggcc ggcacgctcg
    20821 gcggcgccgc cgggcatcac ttcgcccacc gtcgggctgc tgagcggcga gacgatgccg
    20881 atggcgcgaa acagtgccgc gtccggatcg cgcgtggcca gcgtggacag cggcagggtc
    20941 agttcgcgcc cgcggccatc ggcgccgcgg ctgacttcca gcaccaggtc ctgaccgtcc
    21001 agcgcggcgc gtgtgagcac ccagcgcagc gactcgaacg aggccaccgg cgccagcgcc
    21061 tcgccggccg gcgccgcctg gcgcaccagc tcgccgccct gcaggccggc cttgtcggcc
    21121 agcgatccgg ccaccggcgg cgccagcagg gcgcgcggct cctgcacccc gatccagttg
    21181 accagcgcat acagcagcac cgccagcagc aggttggcca ccgggccggc ggcgacgatc
    21241 agggcgcgcg aggccagcgg ctgggtattg aaggcctggt gtcgcaaggc ctcgtccacc
    21301 ggcgcttcgc gctcgtccag catcttgacg tagccgccga acggaatggc gccgatgacg
    21361 aactcggtgt cctgaccggg gcgctggcgc gccggtttga aacgcgcgat ggtcttgccg
    21421 aagccgatgg aaaagcgcag cacccgcaca ccgcgcgcca cggccatgcg gtagtggccc
    21481 cactcgtgca cggcgatcag caggcccagg gccaccacga aggcgatcaa ggtcagcaac
    21541 atgtgggtat tgtcggcgct ggacgacgcc ctcgctcagc cggccagccg ccgcacgcag
    21601 gccagggcgg cagcgcgggc gcgatggtcc agctccagca gcgccgccag gtcgcccggc
    21661 gcgccgggcg cctcggcatc cagggtggcg cggttgacgg cgtggatctg atcgaagcgg
    21721 atgcgccgct gcaggaaggc gtcgaccgcc acttcgttgg ccgcgttcag caccgcgcag
    21781 gtgccgggcg cagcgcgcag cgcctcccag gccagcgcca gcccggggaa gcgctcgcgg
    21841 tggccgtcgg cgtcgatggg ctcgaaactc aggccgccca gggcgtgaaa gtccagcgcg
    21901 gcggcgcccg attcgatgcg ctccggccag gacaggccat aggcgatcgg cacacgcatg
    21961 tcgggcgtgc ccagctgcgc caccaccgag cggtccttgt attgcaccat cgagtggatc
    22021 acgctctgcg ggtggatcac cacctggagc tggtccgggt cgacaccgaa caggtagcgc
    22081 gcctcgatca cctccagcgc cttgttcatc atggtggccg aatccaccga gatcttgcgc
    22141 cccatcaccc agttgggatg ggcgcaggcc tgctcgggcg tgaccgccgc gaggctctcc
    22201 ggcgcgcggg tgcgaaacgg cccgcccgag gcggtgagga tgatcttgtc gatgcgcgcc
    22261 gcccaggtgg ccgggtcctc cggcagcgac tggaagatgg ccgagtgctc gctgtcgatc
    22321 ggcagcaggg tggcgccacc ggcctgcacg gcgcgcagaa acacctcgcc gcccaccacc
    22381 agcgcctcct tgttggccag cagcaggcgc ttgccggcgc gcgccgccgc caggcagggg
    22441 gccaggccgg cggcgccgac gatggccgcc atgacggtgt ctacctcgcc ccagcatgct
    22501 atgttttcaa tagcttccgg cgcttgctcc acctgcgttg gaatgccttt ttgtttgatt
    22561 ttctgcgcca acgcggcggc gtgcggggca ctggccatca ccgccacgcg cgggcgaaag
    22621 cgctcgcact gcgccagcat cgcatcgacc tgcgtggccg ccgtcagcgc caccacctcg
    22681 aagcgctcgg ggtggcgcgc caccacgtcc agcgtgctgg tgccgatcga gccggtgctg
    22741 cccagcaccg ccagccgctg gcgcctcacg caccgcctcc ggccgaccag ctggccagca
    22801 gcatggccag cggcaacgtg ggcagcagcg cgtcgatgcg gtccagcacg ccgccatgac
    22861 cgggcagcaa ctggctggag tccttcatgc cggcgctgcg cttgaccagc gattcgaaca
    22921 gatcgcccac cacgctcatg gcgcacagaa acagcacgcc cgccaccagc agcgccggtc
    22981 cagcctggtg caggcgggtg tagatactgg ctgcacccgg cggctgcgtg cgatccagcc
    23041 acaaccacag cacggccagc gccagcaccc ccagcatgcc gccccacacg ccttcccagc
    23101 tcttgcccgg gctgatcgtg ggcgccagct tgttgccgcc ggtcagccgc ccgcccagcg
    23161 cccggccggc aaagtaggca aacacgtcgg ccacccacac cagcaccatg gccgacagca
    23221 aaaaattgac accgatgcgc cgcgcctgcg ccatggccag ccaggccacg ctcaggagcg
    23281 ccagacccag ggcccagcgc agcgccgctg gcacccgcgc ccagccggcg acgccgcgcc
    23341 ccagcaccca ggcgccaccc agcacccagg ccagagcggc cgccagccac aggccgggca
    23401 gcgggcgctc gaccgcgccc agcgcccaca gccccaggca cagcgccgcg cacagcgcgc
    23461 cggtggccag cgcaccggcg ccggccacgc cattcaggcg cgcccattcc caggccgccg
    23521 cagcgatgaa cagcagactc agcgcgatga agggcgtggc cgacggcgca aacagcgccg
    23581 gcagcaggac ggccagcagc accagcgcgg tgatgacacg ttgtcccagc atggtcggcc
    23641 cctggtcaac cggccgcgcc ggcctgggcc agctgttccg aggtgcgccc gaagcgccgt
    23701 tcgcgcccgg cgtaggcggc cagcgccgca tccagcgcgg cggcgtcgaa atccggccac
    23761 aggcagtcgg tgaaatacag ctcgctgtac gcgcactgcc acagcaggaa gttgctgatg
    23821 cgctgctcgc cgccggtacg gatcagcaga tcggggtcgg gcacatgggc cagggccatg
    23881 gcgccggcca gcgtggcctc ggtgatcggc gcaccgcgct cggccagtcg ggcggcggcc
    23941 tgcacgatgt cccagcggcc gccatagttg aagcacacat tcagcaccat gccggcgttg
    24001 ccggtggtgt cggcctcggc ctgtgccagg gcgtgccgca tgccctcgga caacgcctgc
    24061 cgctcgccca cgaaatgcag tcggatgccg gcgcgctgca actgcggcac ctcgcgcgtc
    24121 agcgccttgc ccagcagttc catcaggcca ctgacctcgt cctgcggccg gctccagttt
    24181 tcggaggaga aggcaaacac cgtcagcacc tgtacgccgc gctcggcgca ggcacggatg
    24241 cagcggcgca gggcgtcgac cccctgccga tgaccggcga tgcgcggcaa caggcgccgc
    24301 cgcgcccagc ggccattgcc atccatcacg atggcgatgt gtcgcggcag ctcgtgggaa
    24361 gtggaagcgt ttgccatgtg cgcggaaccg gtccaggggg ctgcgaggcc ggcccgccca
    24421 tgggccgcgg agcaggccag tacgccctgt ccgagcctcc cggtcgcccg cagcgcccct
    24481 gagcggatgg cgctgccggc gagcgccgga ggtcagactt ccatgatgtc cttttccttg
    24541 gccgccacca gggcgtcgat gtcggccacg tgccggtcag tgactttctg gatatcggcc
    24601 tcggagcgtt tttgctcgtc ctcggtggct tccttgtcct tgaccagctt cttgatggcg
    24661 tcgttggcgt cgcggcgcag gttgcggatg gcgatcttgg cctgctcgcc ctcgtgccgc
    24721 acgatcttgg tcatctcgcg ccggcgttcc tcgctcatgg gcggcatcgg cacgcggatc
    24781 aggtcgccca tgctggccgg gttcaggccc aggtcgcttt cgcggatggc cttctcgatc
    24841 ttggcaccca tgcccttttc ccacggctgc acgctgatgg tgcgcgcatc cagcagcgac
    24901 acgttggcca cctgcgacag tggcacgtgg ttgccgtagt actcgacatg gatggtgtcc
    24961 agcagcgccg ggttggcgcg gccggtgcgg atcttggtca ggttgttctt gaaggcggcg
    25021 atggactggt ccatcttgcc ttcggtgtgc ttcttgatgt ctgcaatgct catgtccatg
    25081 ctccttcggt ggcggccccg gctcagacgt gcaccagggt gccttcgtcg gcacccagca
    25141 ccacgctcct gagcgcgccg ggcttgaaga tcgaaaacac cttgatgggc agcttctggt
    25201 cgcggcacag ggcgaacgcg gtggcgtcca tgatgcccag gttgcggctc atggcctcgt
    25261 cgaagctcag gcgggcgtag cgcgtggcct tgggatcctt gttggggtcg gcggtgtaga
    25321 cgccatccac cttggtcgcc ttgagcacca gttcggcgcc gatctcggca ccgcgcaggg
    25381 cggcggcggt gtcggtggtg aagaacgggt tgccagtgcc ggcggcaaac accaccacct
    25441 tgccttcttc gaggtactgc agcgccttgg gccgcacgta gggttcgacc acctgctcga
    25501 tggcgatcgc cgacatcacg cgcgccgtca ggccgtgctt gttcatggca tcggccagcg
    25561 ccagcgcgtt catcaccgtg gccagcatgc ccatgtagtc ggcggtggcg cggtccatgc
    25621 ccaccgagcc gccggcgacg ccgcgaaaga tgttgccgcc gccgatcacc accgccacct
    25681 gcacgcccat gcgcgtcacc tcggcgatct cgccgacgat gcgctcgatg gtggcgcggt
    25741 tgatgccgaa cgcatcgtcg cccatcaggg cttcgccgga gagcttgagc aggatgcggc
    25801 gatgggcagg cttggctttg gtcatgggcg gggtttgggg gcggcggctg gcggtcgagt
    25861 ttagcggctg atcggcggcg gcgtccggtg cggcgggtac acaaaaaagg cgcgccgcct
    25921 gcgcgacgcg cccgtggcat ggcgcggccg atcaggcctt gccctgcgcg gcggccacct
    25981 gcgcggccac ctcggcggcg aagtcgtcct gcttcttctc gatgccctcg cccaccacgt
    26041 acagcgtgaa gccgctgacc tgggtggctt tttccttcag catctgctcg acgctctgct
    26101 tgtcgttctt cacgaacggc tggttgaaca gcgagacttc cttgaggaat ttctgcaccg
    26161 aaccttcgac catcttggcg gcgatgtcgg ccggcttgcc ggactcggcg gccttggcag
    26221 cggccacgct gcgctcggtt tcgaccagat cggccggcac ctcggcggcg gtgagcgcct
    26281 tgggcttcat ggcggccacg tgcatggcga cgtcgcgcgc ggccacttca tcgccctgga
    26341 actccaccag cacgccaatg cgcgtgccgt gcaggtagct ggtcagctgg gcgccgccgg
    26401 ccttgcgcac gaagcggcgg aagctcatgt tctcgccgat cttgccgatc aggcccttgc
    26461 gcacgtcttc cagcgtgggg ccatagccgt cctgctcgta ggccagtgcg cccagggcgg
    26521 tcacgtcggc cgggttgtgc tcggccacca gcctggcagc ggcctgggcc atggcgatga
    26581 acatgtcgtt cttggtgacg aagtcggttt cgcagttgac ctcgatcatg gcgccggtgg
    26641 tgccggcaat gtgcgcggcg atcacgcctt cggccgtgac gcggctggcg gccttgccgg
    26701 ccttggtgcc cagcttgacg cgcagcagct cctcggcctt ggccatgtcg ccatcggcct
    26761 cggtcagggc cttcttgcac tccatcatgg gggcgtcggt cttggcgcgc agttcggcga
    26821 ccatgcttgc ggtaattgct gccatgtatg attactccag atgtcggtgg ggcggcccgc
    26881 acgggcgggc gccaccccgg gaaacgaaac attgaaaaag gggcctgtgc gcccctttgc
    26941 cagcggtcgc caggcgggcg acgcaggtgg gcgatcaggc ggcgccgtcg tcctcgactt
    27001 cgacgaactc gtcctcgcct tcggcctgca cggccttgac cacgtcattg acggcgttgg
    27061 ccttgccttc cagcacggcg tcggccagcg cacgggcgta cagcgtgacg gcacgcgcgg
    27121 agtcgtcgtt gcccggaatc acgtagtcga taccgtccgg gttgtggttg gagtcgacca
    27181 cgccgatcag cggcacgccc agcttcttgg cttccgagac ggcgattttg tggtagccga
    27241 cgtcgatcac gaagatggcg tccggcagcg cgcccatgtc ctggatgccg ccgatgtcgc
    27301 gttcgagctt ttccatctcg cgcgtgaaca tcagctgctc cttcttgctc atgctgtcca
    27361 gaccggcttc ctgctgggct tgcatgtcct tgaggcgctt gatggaggtc ttgacggtct
    27421 tgaagttggt cagcatgccg cccagccagc gctgctcgac gtagggcatg ccgcagcggc
    27481 gggcttcctc ggcgacgatc tcacgcgcct ggcgcttggt gccgacgaac aggatggtgc
    27541 cgcggttggc cgccagctgg cgcgcgaatt tctgcgcttc ctggagcatc ggcagcgatt
    27601 tttccaggtt gatgatgtgg atcttgttgc gatggccgaa gatgaaaggg gccatcttgg
    27661 ggttccagaa gcgcgtctgg tggccaaagt gcacaccggc ttccagcatc tgacgcatgg
    27721 tcacggacat ggaaattacc ttctcaaggt tgggtctgaa atccagcccc ctcaatcacc
    27781 ggccgggccg ttgaaacggc cggcagcgac acctcgtcgg
//
LOCUS       DAOQTZ010000024        27664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2034_length_27664_cov_14.219530, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000024 DAOQTZ010000000
VERSION     DAOQTZ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 27664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27664
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_27664_cov_14.219530"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PKN51_04580"
     CDS             complement(<1..435)
                     /locus_tag="PKN51_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNT84408.1"
                     /translation="MSMRTHYCGLVTEALIGQTVSLCGWVNRRRDHGGVIFVDLRDRE
                     GYVQVVCDPDRPGMFSVAEDLRNEFCIQVKGLVRARPEGTSNDNLKSGKIEVLCHELV
                     VLNPSVTPPFQLDDDNLSETTRLTHRVLDLRRPAMQRNLMLRY"
     gene            complement(474..1193)
                     /locus_tag="PKN51_04585"
     CDS             complement(474..1193)
                     /locus_tag="PKN51_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNT84409.1"
                     /translation="MMSRLRQYFITGLLVWLPMGVTVWVLLWLLGILDGIFLGVLSAA
                     EAMVPGLGPLANRLRHVPGLGVILVAIVIFSTGVFVANMFGQWSVRQWDRLMTRIPVV
                     RSIYSGVKQVSDTLFSGNGQAFSKALLIQYPRPGAWTIAFVTGKPEGEVSCHLPPDCI
                     SVYVPTTPNPTSGFFLMVPRADAIELRMSVDEALKYVISMGVVAPPQRQPCPPGEAVP
                     EPLQAQVVSAPADARQSPPAG"
     gene            complement(1215..1514)
                     /locus_tag="PKN51_04590"
     CDS             complement(1215..1514)
                     /locus_tag="PKN51_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT84410.1"
                     /translation="MPIYAYKCAACGFAKDVLQKMSDPPLAECPSCGQAAFEKQLTAA
                     GFQLKGSGWYVTDFRDGGSKGGAAAPSPAPAAAQAAAPAPAAPAPASSSPAPAGS"
     gene            complement(1620..3089)
                     /locus_tag="PKN51_04595"
     CDS             complement(1620..3089)
                     /locus_tag="PKN51_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNT84411.1"
                     /translation="MLISFIVLYLVISIAIGLYAATRVKNTADYAVAGRSLPLAVVIA
                     TTFATWFGSETVLGAPAQFVKEGLHGTVEDPFGAGLCLVLVGLFFARKLYLRHIITIG
                     DYYRQRYGRVVEVLCSLIIIFSYLGWVGAQITALGLVFNLLSQGAVSLTMGMVIGTLV
                     VLVYTLYGGMWSVALTDFLQMIVITAGLLAIAWYAGDMAGGADKVVAYAAREGKFNFL
                     PTGGLKEWVFFFAAGITMMLGSIPQQDVFQRVMSSRDVKTAQRGPVIGGLLYIAFAFV
                     PMFIVMAALLVMPETAALLDDDPQKVLPTLVMGHMPVLLQVAFFGALLSAIMSTASAT
                     LLAPATTFVENILRNLRPGMNDAETLKAMRISVLVFTLCVLVYSITMEGSSIYELVSG
                     AYQVPLVGAFVPLAFGVYWRRATTQGALLAVVMGIGVWVLFVASPMLNEAFPQQLAGV
                     LAAIVGMVFGSLAPQWLPNHKGYVHHLVEREAVTGEPAR"
     gene            complement(3211..4779)
                     /gene="ubiB"
                     /locus_tag="PKN51_04600"
     CDS             complement(3211..4779)
                     /gene="ubiB"
                     /locus_tag="PKN51_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNT84412.1"
                     /translation="MTRVLRATTIVWVALRHGLDELVLTSFEHPGLRLLARIATLGRD
                     LSAPRGQRLREALQRLGPIFVKFGQVLSTRRDLLPPDIADELALLQDRVPPFPPEAAV
                     ATIERAFRKPLGELFTHFERQPVASASIAQVHFATLRDAGGREREVAVKVLRPGMLKV
                     IEQDLQLIRMMATWVERLSADGKRLKPREVVAEFDKYLHDELDLMREAASAAQLRRNM
                     AGLNLVLIPEMIWELARPDVLVMERMQGIPISQVERLREAGVDIPRLARDGVTIFFTQ
                     VFRDGFFHADMHPGNIMVSVDPATFGRYISLDFGIVGTLTEVDKEYLAQNFTAFFRRD
                     YKRVAELHIESGWVPAGTRVDELESAVRSVCEPYFDRPLKDISLGMVLMRLFQTSRRF
                     NVEIQPQLVLLQKTLLNIEGLGRQLDPELDLWSTAKPFLEQWMLEQIGPQRLWREFKS
                     QAPYYAKMLPDLPRLLHAYLQQRPSDELRRELHELREEQRRTRRLLRATAVGGLLLGL
                     ALTGALLAWRGHGF"
     gene            complement(4799..5341)
                     /locus_tag="PKN51_04605"
     CDS             complement(4799..5341)
                     /locus_tag="PKN51_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84413.1"
                     /translation="MTMKSPFSLVAEAFGKLGEQLKAPDWLVDEMQHRVVLFLNHVLM
                     QEPEAQARLARQQGRVVRVQWRDFNLQLAATPAGLCERVPEATPDLLLTVTDSSPLDL
                     ARHAVRGERPGVRIEGDVQFAAEVNWLVDHVRWDLEEDLSRLIGDAPAHQLARLGSTL
                     AQGLRRFIASLPGQDRAGGA"
     gene            complement(5474..6478)
                     /locus_tag="PKN51_04610"
     CDS             complement(5474..6478)
                     /locus_tag="PKN51_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNT84414.1"
                     /translation="MNLKKLVSHFFTLLLAVVLTGAFSFEAEAKRLGGGKSLGRQSSN
                     VTQREAAGPGTPVAPAQSSATAGAAAGGAAAAGAAGAAARKPWGSMLGGLAAGLGLAW
                     LAHALGLGQAFGNVLLILLLGMVVLAVIGMLRRGRGAAAAGGPGMAYQGAGAAQPITP
                     RQYNPTKVGNDASARPWEGQAAPFDAGGASGGSIIGSGLRGAQGWGVPADFDIAGFTE
                     AAKRNFITLQDAWDRSDIPALRAMMTDEMLAEIQSQLAERERGAPGQANQTEVVMLEA
                     QLLGIEELADVYMASVEFSGMIREDASAGPSPFREVWNMTKPRQGRGGWLVAGVQALQ
                     "
     gene            complement(6540..7274)
                     /gene="ubiE"
                     /locus_tag="PKN51_04615"
     CDS             complement(6540..7274)
                     /gene="ubiE"
                     /locus_tag="PKN51_04615"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNT84415.1"
                     /translation="MATTHFGFQSVDEQDKARRVRGVFDSVAARYDLMNDLMSGGLHR
                     AWKAYAVMVANLQPGQSVLDIAGGTGDLALAFARRVGPHGQVVHTDINEAMLRTGRDR
                     LLDAGVILPTTVCDAEALPFPDERFDVVSVAFGLRNMTHKDQALREMARVLKPGGRLL
                     VLEFSQVARPLRKAYDLYSFQVLPRLGRLVAGDADSYRYLAESIRMHPNQEELKALMK
                     ANGFGHVDYHSMTAGVVALHVGIKCG"
     gene            7380..9032
                     /locus_tag="PKN51_04620"
     CDS             7380..9032
                     /locus_tag="PKN51_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT84416.1"
                     /translation="MPRPSQLLHPQREPARPLPAATVLLLRDTPAGIEVLMTRRSLNA
                     SFAPGAYVFPGGGIDPQDAAAHGQATCRPTQSQAHLTQAIAAIRESFEELGVLLARRA
                     DGTPADASDVAALDRHAPLAAQCAARGLTLAAADVHMLAHWITDRDLPRRFDVPFLVA
                     RMPDGQTPVADDAEQFEPVWVRPADALARHEAGQFFIIFPTIRTLQRLQHYPDVAAVL
                     AACASERPLWASCPRAGWLAGKEARYMEHEMPFGELALVTPDGQLLHALDWQSEQPVP
                     LLKNVQRLTAPNPGFMTGPGTNSYLVGDPRSGYIAIDPGPADPAHVERLWRAAGGHIR
                     AIVCTHSHPDHSPGALPLQALCQPPPPILGLPSLPTARAHSQFQPDRALQDGERLALT
                     GQAPDGEITTHTLRAIHTPGHAANHVCLLLEEDGLLFSGDHILNGSTTVIDPPDGDMT
                     AYLESLDKLDAACAEAQAHFILPAHGHVLGQARDTIAHLKAHRLAREAKVLAVMKADP
                     HGTLDDWVAKAYDDVPERVWPVARRSLLAHVERLRAMAPGND"
     gene            complement(9364..9682)
                     /gene="rnpB"
                     /locus_tag="PKN51_04625"
     ncRNA           complement(9364..9682)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKN51_04625"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(9732..9896)
                     /locus_tag="PKN51_04630"
     CDS             complement(9732..9896)
                     /locus_tag="PKN51_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84417.1"
                     /translation="MTLFDSTMLMLGVSCLMMLIGFATRESRWGLGLMVLGTAGALLL
                     IAYTILAMLE"
     gene            10031..10960
                     /gene="cysK"
                     /locus_tag="PKN51_04635"
     CDS             10031..10960
                     /gene="cysK"
                     /locus_tag="PKN51_04635"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNT84418.1"
                     /translation="MPADNVLQTIGRTPHVRIRRLFGPEARVWVKCERANPGGSIKDR
                     IALAMIEAAEQSGQLQPGSTIIEPTSGNTGVGLAMVAAVKGYRLVLVMPDSMSVERRR
                     LMLAYGASFDLTPRDQGMKGAIARAEELAAQLRAAGQGAWIPQQFDNPANAAVHEQTT
                     AQEILADFPDGLDALITGVGTGGHLTGCARVLKARWPQLKVFAVEPDQSPVISGGAPG
                     PHPIQGLGAGFIPKNLDTALLDGVIQVGAEAARDYGRRCAREEGLLVGISSGAALAAI
                     AQKLPELPAGSRILGFNYDTGERYLSVDGYLPT"
     gene            11173..11445
                     /locus_tag="PKN51_04640"
     CDS             11173..11445
                     /locus_tag="PKN51_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84419.1"
                     /translation="MKFIELPDVLLNGKPERLAHAVRVRDERNAIVMPGVVNGRTAVV
                     NYLRDARGQVREVWILSPQEAALPLKPVPTTRRPAPSADATASYTH"
     gene            11534..12874
                     /gene="miaB"
                     /locus_tag="PKN51_04645"
     CDS             11534..12874
                     /gene="miaB"
                     /locus_tag="PKN51_04645"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNT84420.1"
                     /translation="MSRKVFIKTFGCQMNEYDSAKMADVLGAAQGYEPTDDPEQADLI
                     LFNTCSVREKAQEKVFSDLGRVKHLKARGTLIGVGGCVASQEGEAIIARAPYVDVVFG
                     PQTLHRLPELLAERQKQNAPQVDIRFPEIEKFDHLPPARVDGASAFVSIMEGCSKYCS
                     YCVVPYTRGEEFSRPLDDVLTEIAGLADQGVKEVTLLGQNVNAYRGPMGDSSEIADFA
                     LLIEVVAEIPGIERIRYTTSHPNEFTPRLIEVYGKVPKLVSHLHLPVQHGSDRILMAM
                     KRGYTAMEYKSIVRKLRAVRPDLSLSSDFIVGFPGETEQDFDKLMKLIEDVGYDASFS
                     FIFSPRPGTPAANLHDDTPHAVKLARLQHLQAVVEQNVRAISASRVGTRQRILVEGPS
                     RRNPNELMGRTECNRIVNFDAGAQGARLVGQMIEVDITEALPHSLRARLASAAG"
     gene            12969..14516
                     /locus_tag="PKN51_04650"
     CDS             12969..14516
                     /locus_tag="PKN51_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT84421.1"
                     /translation="MPPPRLELTQIRKRYPGVLANDDVSLTVQPGEIHAVLGENGAGK
                     STLMKIIYGAVKPDAGELRFDGQPVRIANPQQARRLGIAMVFQHFSLFETLSVAENVW
                     LGLDKALPLAEVTRRIDQTAAAYGLEIDPARPVHTLSVGEMQRVEIIRALLTEPRLLI
                     LDEPTSVLTPQAVDKLFVTLRRLAGEGCSILYISHKLHEIRALCSACTVMRAGRVTGV
                     CDPRQESNASLSRLMIGAEPPELAHRPQALGARVLQVERLDLKRESPFGVDLVDLSLQ
                     VRAGEVVGIAGVSGNGQRELLFALSGEDRRAPAASILIADQAAGRLSPSRRRALGLHF
                     VPEERLGRGAVPDMSLAHNLLLTRREAVGPLGWLRTGTLRAQAQAIIERFRVKAAGPQ
                     APARSLSGGNLQKFIMGREIDARPRLLIVSQPTWGVDVGAAAQIRGELLALRDAGCAV
                     LVVSEELDELFEVCDRLHVIAKGRLSPSIARQDATVERIGEWMSGLWHGDVQQHLAQM
                     EAGHVAA"
     gene            14503..15567
                     /locus_tag="PKN51_04655"
     CDS             14503..15567
                     /locus_tag="PKN51_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT84422.1"
                     /translation="MWRLEPRPAPSRAWTWGSPLLALALIVLIGVALFTALGKDPVRG
                     LLVFFWEPIKSGYALGELSVKATPLLLIALGLAVCFRSNVWNIGAEGQFIVGAIAAGG
                     VALLADKDSGRWIVGAVLLAGVLGGMAWAAVTAFLRDRFNAGEILVSLMLVYVAIQWL
                     GYLVYGPWKDPAGYNFPQTRSFEAVAQVPRLLNGSRAHIGVLIALAGAALLWVYLFRT
                     RSGFAQQVGGLAPAAARYAGFSSRRALWTALLVSGGTAGLAGALEVAGPLRQLTPHVP
                     AGYGFAAIIVAFVGRLHPFGMVLAAILMSMFYIGGELAQSRLGLPKSLTGVFQGLLLF
                     ALLACDVLVNHRLRRRGGSH"
     gene            15567..16487
                     /locus_tag="PKN51_04660"
     CDS             15567..16487
                     /locus_tag="PKN51_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT84423.1"
                     /translation="MEAYALLLAATLNAGTVLAIAALGLLINEKAGIVNLGAEGIMLC
                     AAVAGFAAVVHSGSDGLGFAAGMAAGALLAAKFGWLVIWLGTNQYATGLALSLFGVGF
                     SAFVGIGYVQARLPERPRFNGLGLGDLPLIGPALFRHHPLVYGAMALALGLIWFLYRT
                     RGGLVLRSVGESPQAAHALGYPVRRIRLLAVVAGGALCGLAGAYLSVIYTPLWVEGMV
                     AGKGWIALALTTFATWRPARVLLGAYLFGGVTMLQFHLQGQGVQLPSQFLTMLPYLAT
                     IVVLALISRNPTWIRVNMPASLGKPFHPGA"
     gene            16706..17869
                     /locus_tag="PKN51_04665"
     CDS             16706..17869
                     /locus_tag="PKN51_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT84424.1"
                     /translation="MLDPNKRSLLKLAALAAAASALVACSKKEEPAPTAAAPAAPAAA
                     PAPAKPEPLKVSFAYIGPVGDGGWTFAHDNARKAVEKEFGDRVVTSYVESVPESADAE
                     RVIRDMVGQGNKLIFGTTFGYMESMVKVAGDHPDVKFEHATGYKTAPNLRTYDSRTYE
                     GAYMAGVIAGRMTQSNVLGVVGSVPIPEVLRNLNSFTLGAQSTNPKITTKVVWVNDWF
                     NPPKETEAATALLNAGADVLFQNTDSPAVLKTAQEKGKRAFGWDSDMTAYGPKAHLGS
                     AIINWAPYYIKAVGEALEGKWSTGQAWWGVKEGAIDLVSLAEDVPADAKAKVEEVKAG
                     LKAGTFHIWKGPISDNTGKQVLAADQVADDKFLTGINFYVKGVEGKVPGGEKK"
     gene            complement(18044..18724)
                     /locus_tag="PKN51_04670"
     CDS             complement(18044..18724)
                     /locus_tag="PKN51_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNT84425.1"
                     /translation="MATPIDIDFVSDIACPWCAVGLASLQQALARCADAVQVRLRVQP
                     FELNPAMPPEGEDLGEHLTRKYGSTPAQQAQMRAVIAERGAALGFAFHPEGRGRVVNT
                     FDGHRLLHWAGRQGADAQLALKRALLQAYHGRAERVDQAPVLLAAVRQAGLDEARAAQ
                     ILAGDEFAAEVRQAERQWQDAGIHAVPAVILNQRHLISGGQPPEVFEQALRQLAAADG
                     SGSARRDA"
     gene            complement(18915..19844)
                     /locus_tag="PKN51_04675"
     CDS             complement(18915..19844)
                     /locus_tag="PKN51_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNT84426.1"
                     /translation="MNRTPDPGPGQRAASAGPTPLDAAAFPAALPPAERLRLASGQRL
                     TTPGGQGELVWHRWGTRRAGQPPVVLLHGGSGSWTHWVKNIAPLLDAGRQVLAADLPG
                     FGDSAPPTQGSDADAMVEPLANGLRQLQPGEAVDLVGFSLGGLTAALLLAEHPALARR
                     LVLVGAPAMGVVPGRQFELRAWRHLPSPAAQAEVHRHNLAVLMLHDPGLIDGLALQLH
                     IANVQRDRLPRRRLAHTDILARRLPQVACPVHAIYGVHDAIYRQHIHALEGAFARSAP
                     DFRGLQLIEGAGHWVQFEAPAAFDAALRRALGD"
     gene            complement(19841..20266)
                     /locus_tag="PKN51_04680"
     CDS             complement(19841..20266)
                     /locus_tag="PKN51_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNT84427.1"
                     /translation="MADPMERQRIDKWLWAARFYKTRALAVQEIERGRVQVAGQTVKP
                     ARELRPGDVLSLRQGVVQREVVVRALSPQRGPAPVAQQLYEETPASVAARQQAASARR
                     LAPEPALSIAQGRPTKRDRRALDQQQRADWGERWSAGIE"
     gene            20325..22865
                     /locus_tag="PKN51_04685"
     CDS             20325..22865
                     /locus_tag="PKN51_04685"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNT84428.1"
                     /translation="MPQPSHPPGLSGDEARRRLAEHGPNTIDERERRSLAGTLREIAR
                     EPMFLLLLVAAAIYLLVGEPGEGLLLGGFALLTVGLVIFQQRRGERALDALRELAAPQ
                     AQVLREGRVQRIAVREIVPGDWIVLGEGERVAADAVLRQAQALTVDESLLTGESVPVR
                     KCASHAPLVEAATDRPGGDDLPQVFAGTLITAGHGLAEVCATGRQTQMGRIGAALATI
                     EPAATPLQRSLHRLARWFGALALTGSALLVLWHGLRGGDWVQGLLSGIALGMAMLPEE
                     FPMALAVFLALGSWRLARIQVLARQPAVVEALGATTLLCVDKTGTLTENRMALRRLVT
                     QEVDVTVQAGAPLPASVHRLLACAMLASRRGTSDPMDRALLLHGDQALAGTEHLHPDW
                     QLEREYALTPALPAMTQTWLDDAGRRHVAAKGAPEAVIELCHLPPAAAARLLAQVAAL
                     AAAGLRVLAVAEGQGSTAETAPDPHDYDLRLLGLLGFEDPLRASVPAAVAQAHGAGIA
                     VAMITGDHAATALAIARQAGIDTAAGALTGAQLAALDDAALSAAVRRVRVYARVLPEQ
                     KLRLVEAMQRNGETVAMTGDGVNDAPALKAAHIGIAMGGRGTDVAREAAGLVLLDEDF
                     SRIVGGVRMGRRIFDNLRKAMVYIAAIHVPIAGLALLPVLLGLPPLLLPAHVVLTEMV
                     IDPICSLAFEGAPEDPRLMQRPPRRAAEGLVGRSLLAQAAVQGASVLAATLAIYLQAL
                     HGGQSADVARTLAVIGLMAGNLLLVAVNLSAGLGWRALLARGARAFWAVAAIATAVLG
                     LALALPQARALLRFGPVSLAQVGLALLAVALAVVVGARFGRALERSTA"
     gene            22907..24412
                     /locus_tag="PKN51_04690"
     CDS             22907..24412
                     /locus_tag="PKN51_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT84429.1"
                     /translation="MPATLSPAPSALPRDASRILELAARSLFELFEDASEGMLLVDRD
                     ARVVWINDPYRRFLPALGFEREEDFVGHPVSDVVQNTQMHQVLRTGKPILIDLLSNRA
                     GTFAVSRIPLRDAAGEVIGALGIVLFDQTSHSLQPLLAKFARLQQELDDAQRELAAQR
                     RQQGDGQRRARYTLASFVGSSPAASEVKRQARRAAQSSSPVLLLGETGTGKELLAHGI
                     HAASPRAGGPFVSVNIAAVPDTLLEAEFFGVAPGAYTGADRKGREGKFRLADGGTLFL
                     DEIGDMPPGLQAKLLRALQDGEIEPLGSNRLVPFDARIIAATSRDLPQLVRAGQFRED
                     LYYRLNVLPIQVPPLRTRRGDIPALIEVLAEDMLHRGSQPPELTPAAVALLAAQHWRG
                     NIRELRNVLEQAALRADAGALDAEPMEALLRQSGLSLVQPALPPEPPPQDAAALLRPL
                     GEQVAELERRAIAAALQATHGNKLAAARLLGISRATLYGRLLEPGDAPQKP"
     gene            24575..25762
                     /locus_tag="PKN51_04695"
     CDS             24575..25762
                     /locus_tag="PKN51_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNT84430.1"
                     /translation="MQRRSLIALASFAVLTGAPAFAAGDIKIAHVYSKTGPLEAYGKQ
                     TQTGLMMGLQYATGGTMQVNGRKITVIERDDQGKPDLGKSLLAAAYADDKVDLAVGPT
                     SSGVALAMLPVAEEYKKILLVEPAVADAITGDKWNKYIFRTGRNSSQDAISNAVAMDK
                     DGVSVATLAQDYAFGRDGVKAFKDALKKARIVHEEYLPQNTTDFTAGIQRLIDALKDK
                     PGRKAIEVIWAGATPPFNALAAADLKKRYGIEVFTGGNILPAMASYKNFPGMEGATYY
                     YFGIPKNPVNEALVAAHYKEFKAPPDFFTAGGFSAAMAIVTALKATGGDTATNKLIKT
                     MEGMSFDTPKGKMTFRKDDHQAMQSMYHFRIKADPAFTWGVPELVKEIKPEEMQVPIR
                     NKR"
     gene            25892..26719
                     /locus_tag="PKN51_04700"
     CDS             25892..26719
                     /locus_tag="PKN51_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT84431.1"
                     /translation="MLETEDLTIRFGGHVAVNAVTCRFEPGTLTAIVGPNGAGKTTYF
                     NLISGQLKASAGRVLLNGRDLSALPASARTQAGLGRAFQLTNLFPNLSVLENVRLAVQ
                     AAHRIGLNLWRIWSDQRELTQRALEILESVALLDRQGMPVAGLPHGDQRKLEVALLMA
                     LDPQVYMFDEPTAGMSHDEAPVILDLIRRLKQDRSKTILLVEHKMDVVRELADRIIVL
                     TNGQLVADGEPAEVIASPVVQEAYLGLAPQDAFGSTDARPHPGLPPSGGRSLGDTAP"
     gene            26716..27441
                     /locus_tag="PKN51_04705"
     CDS             26716..27441
                     /locus_tag="PKN51_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT84432.1"
                     /translation="MSETLLTLEGVHTHIGAYHILHGVDLQVPRGQLTMLLGRNGAGK
                     TTTLRTIMGLWQASAGRIGFNGQDITRRHTPQIAQLGIAYVPENMGIFADLTVKENMV
                     LAARGARSAAQIDGERLAWIFRLFPAVEKFWHHPAGKLSGGQKQMLAVSRAIVEPREL
                     LIVDEPSKGLAPAIINNMIDAFQQLKASGVTILLVEQNLSFARRLGDRVAVMDNGRIV
                     HAGSMQALAEDAALQQRLLGLSL"
     gene            27519..>27664
                     /locus_tag="PKN51_04710"
     CDS             27519..>27664
                     /locus_tag="PKN51_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT84433.1"
                     /translation="MKLDFDYQPLLLVPVLALLALPLVGSGSTWLTLTVAGLAMGMII
                     FIIAS"
BASE COUNT         4263 a         9834 c         9580 g         3987 t
ORIGIN      
        1 gtagcgcagc atcaggttgc gctgcatggc cgggcggcgc aggtcgagca cgcggtgcgt
       61 caggcgggtg gtttccgaca ggttgtcgtc atcgagctgg aacggcggcg tgaccgaggg
      121 gttgagcacc accagctcgt ggcacagcac ctcgatcttg ccgctcttga ggttgtcgtt
      181 gctggtgccc tcggggcgcg cgcgcaccag gcccttgacc tggatgcaga actcgttgcg
      241 caggtcctcg gcaacgctga acatcccggg ccggtcgggg tcgcacacca cctgcacata
      301 gccttcacga tcgcgcagat cgacgaagat cacgccgccg tggtcgcgcc ggcggttgac
      361 ccagccgcac aggctcacgg tctgaccgat cagggcctcg gtcacgaggc cgcagtagtg
      421 ggtacgcatc gacatggaat ggactttcgg aaaacggttc agggccggcc aggtcagccg
      481 gcaggaggtg attgccgggc atcggcggga gcgctgacga cctgcgcctg cagcggctcg
      541 ggcacggcct cgccgggtgg acacggctgg cgctggggcg gcgccaccac gcccatggaa
      601 atcacgtact tcagcgcctc gtccacgctc atgcgcagct cgatggcgtc ggcccgcggc
      661 accatcagga agaagcccga agtggggttg ggcgtggtcg gcacatagac gctgatgcag
      721 tcgggcggca ggtggcagct gacctcgccc tccggcttgc cggtgacgaa ggcgatggtc
      781 caggcaccgg ggcgcgggta ctggatcagc agcgccttgg aaaaggcctg accgttgcct
      841 gaaaacaggg tgtcggacac ctgcttgacg ccggaataga tgctgcgcac caccggaatg
      901 cgcgtcatca gccggtccca ctgccgtacc gaccactggc cgaacatgtt ggccacgaac
      961 acgccggtgc tgaagatgac gatggccacc aggatcacac ccagaccggg cacgtggcgc
     1021 agtcggttgg ccagcggccc caggcccggc accatggctt cggccgccga cagcacgccc
     1081 aggaagatgc catccaggat gccgagcagc cacagcagca cccacaccgt cacgcccatg
     1141 ggcagccaca ccagcaggcc ggtgatgaag tattgccgca ggcgggacat catgggatgc
     1201 ggttctcttg aggatcagct gccggcgggc gctgggctgc tgctggcggg ggccggggcg
     1261 gcgggcgcgg gcgccgcggc ttgcgccgcc gcgggtgcgg gcgacggcgc ggccgcgccc
     1321 cctttggagc cgccgtcgcg gaaatcggtc acgtaccagc ccgagccctt gagctgaaag
     1381 ccggcggcgg tgagctgctt ctcgaacgcc gcctgtccgc aggacgggca ctcggccagg
     1441 ggcgggtcgg acatcttctg cagcacgtcc ttggcaaaac cgcaggcggc gcacttgtag
     1501 gcgtagatgg gcatggcgaa cgcgaaatca ggctggggca aaaaactttt cattataaaa
     1561 gttcggccct tgccacgccg ccgtggctgc gacgcccctg tggtgccccc tgcggtgcgt
     1621 cagcgcgccg gctcgcccgt gacggcctcg cgttccacca ggtggtggac gtagcccttg
     1681 tggttgggca gccattgcgg cgccagcgaa ccgaacacca tgccgacgat cgccgccagc
     1741 acgccggcca gctgctgtgg gaaggcctcg ttcagcatcg ggctggccac gaacagcacc
     1801 cacacgccga tgcccatcac caccgccagc agcgcgccct gggtggtggc gcgccgccag
     1861 tagacgccaa acgccagcgg cacgaaggcg cccaccagtg gcacctggta ggcgcccgag
     1921 accagctcgt agatggacga gccttccatg gtgatcgaat agaccagcac gcacagggtg
     1981 aacaccagca cgctgatgcg catggccttc agggtttcgg cgtcgttcat gcccgggcgc
     2041 aggttgcgca ggatgttttc gacgaaggtg gtggccggcg ccagcagcgt ggccgaggcg
     2101 gtggacatga tggccgacag cagggcgccg aagaaggcca cctgcagcag caccggcatg
     2161 tggcccatca ccagcgtggg cagcaccttc tgcgggtcgt cgtccagcag cgccgcggtc
     2221 tcgggcatca ccagcagcgc ggccatgacg atgaacatcg gcacgaaggc gaaggcgatg
     2281 tacagcagcc cgccgatcac cggcccgcgc tgggcggtct tgacgtcgcg tgaggacatc
     2341 acgcgctgga acacgtcctg ctgcggaatg gagcccagca tcatggtgat gccggcggca
     2401 aagaagaaca cccattcctt caggccgcca gtgggcagga agttgaactt gccttcgcgt
     2461 gcggcgtagg ccaccacctt gtcggcaccg ccggccatgt cgccggcgta ccaggcgatg
     2521 gccagcagac cggcggtgat gacgatcatt tgcaggaaat cggtcagcgc caccgaccac
     2581 atgccgccat acagcgtgta gaccagcacc accagggtgc cgatgaccat gcccatggtg
     2641 agcgacaccg cgccctggct gagcaggttg aacaccagac cgagcgccgt gatctgcgcg
     2701 cccacccagc ccaggtagct gaagatgatg atcagcgagc acagcacctc gaccacccga
     2761 ccgtagcgct ggcggtagta gtcgccgatg gtgatgatgt ggcgcaggta cagcttgcgg
     2821 gcgaagaaca ggcccaccag caccagacac aggccggcac cgaacgggtc ttccacggtg
     2881 ccgtgcaggc cttccttgac gaactgcgcc ggcgcgccca gcaccgtttc ggaaccgaac
     2941 caggtcgcga aggtggtggc gatgaccacc gccagcggca ggctgcgccc ggccaccgcg
     3001 tagtcggccg tgttcttcac ccgcgtggcg gcgtacaggc cgatggcgat cgagatcacc
     3061 aggtacagca cgatgaagct gatcagcatg cccgtgccct ccgcgcggtc atgaccaccg
     3121 tccgttcacc gatcatcaca ccctccgagt tttgctacgt tgttgatagc tgctggcaca
     3181 tacccagaaa gcgccgcaga ccgatttgat tcaaaaccca tgcccccgcc aggccagcag
     3241 cgcgccggtc agcgccagcc ccagcagcag gccacccacc gccgtggcac gcagcagacg
     3301 gcgcgtgcgg cgctgttcct cgcgcagctc atgcagctcg cggcgcagct cgtcgctcgg
     3361 ccgctgctgc agataggcat gcagcagccg cggcagatcg ggcagcattt tggcgtagta
     3421 cggcgcctgg ctcttgaact cgcgccacag ccgctgcggg ccgatctgct cgagcatcca
     3481 ttgctccaga aacggcttgg cggtgctcca caggtccagt tcggggtcga gctggcgccc
     3541 caggccctcg atgttcagca gcgtcttctg cagcagcacc agctgtggct ggatttcgac
     3601 gttgaagcgg cgcgaggtct ggaacaggcg catcagcacc atgccgagcg agatgtcctt
     3661 cagcggccga tcgaagtagg gctcgcacac gctgcgcacg gccgattcga gctcgtccac
     3721 gcgcgtgccg gccggcaccc agccgctttc gatgtgcagc tcggccacgc gcttgtagtc
     3781 gcggcggaag aaggcggtga agttttgcgc caggtattcc ttgtcgacct cggtcagcgt
     3841 gccgacgatg ccgaaatcga gcgagatgta gcgcccgaag gtggccggat cgacgctgac
     3901 catgatgttg cccgggtgca tgtcggcgtg gaagaagccg tcgcgaaaca cctgggtgaa
     3961 gaagatggtc acgccgtcgc gcgccagtct ggggatgtcg accccggcct cgcgcaggcg
     4021 ctcgacctgg ctgatcggaa tgccctgcat gcgctccatc accagcacgt cgggccgcgc
     4081 cagctcccag atcatctccg ggatcagcac caggttcagc cccgccatgt tgcgccgcag
     4141 ctgcgcggcg ctggcggctt cgcgcatcag gtccagctcg tcgtgcaggt atttgtcgaa
     4201 ttcggccacc acctcgcgcg gcttgaggcg tttgccatcg gccgacaggc gttcgaccca
     4261 ggtggccatc atgcggatca gctgcagatc ctgctcgatc accttcagca tgcccgggcg
     4321 cagcaccttc accgccacct cgcgctcgcg cccgccggcg tcgcgcaggg tggcaaagtg
     4381 cacctgggcg atggaggcgc tggccaccgg ctggcgctcg aaatgcgtga acagctcgcc
     4441 cagcggcttg cggaaggcac gctcgatggt ggccaccgcc gcttcgggcg gaaacggtgg
     4501 cacgcggtcc tgcagcaggg ccagttcgtc ggcaatgtcg ggcggcagca ggtcgcgccg
     4561 cgtcgacagc acctggccga acttgacgaa gatcggcccc aggcgctgca gcgcctcacg
     4621 caggcgctgg ccccgcggcg ccgacagatc gcgcccgagg gtggcgatgc gcgccagcag
     4681 gcgcaggccc gggtgctcga agctggtcag caccagttcg tccagcccat ggcgcagagc
     4741 cacccacacg atggtggtgg cccgcagcac gcgcgtcacc ggcgcctccc cgacgcgctc
     4801 atgcgccgcc tgcccggtcc tgaccgggca ggctggcgat gaacctgcgc agcccctgcg
     4861 ccagggtgct gcccaggcgt gccagctggt gcgcgggagc gtcgccgatc agccgcgaca
     4921 ggtcttcctc cagatcccag cgcacgtggt cgaccagcca gttgacttcg gcggcgaact
     4981 gcacatcgcc ctcgatgcgc accccgggcc gctcgccgcg cacggcgtgg cgcgccagat
     5041 cgagcggcga gctgtcggtg accgtgagca gcaggtcggg cgtggcctcg ggcacgcgct
     5101 cgcacagtcc ggccggggtg gccgccagct gcaggttgaa gtcgcgccac tgcacgcgca
     5161 ccacgcgacc ctgctggcgc gccagccggg cctgggcttc gggctcctgc atcagcacgt
     5221 ggttgaggaa cagcaccacg cggtgctgca tctcgtcgac cagccagtcg ggcgccttca
     5281 gctgctcgcc caacttgccg aatgcctcgg ccaccagtga gaagggggac ttcatcgtca
     5341 tgggggcgat tattcctgac aaatggcggc gtcggccgcc cagatgcacg cgttcttcct
     5401 gcctggcacg gacacaaaaa agcccggcaa gccgggcttc ggggcgggcc gggccggtac
     5461 gcccggccgg tcgtcactgc agtgcctgca cccccgccac cagccagccg ccgcggccct
     5521 ggcgcggctt ggtcatgttc cagacctcgc ggaacgggct cgggccggcc gaggcgtcct
     5581 cgcggatcat gccgctgaac tcgacgctgg ccatgtacac gtcggccagc tcctcgatgc
     5641 ccagcaactg cgcctccagc atcaccacct cggtctggtt ggcctgaccc ggcgcaccgc
     5701 gctcgcgctc ggccagctgg gactggattt cggccagcat ctcgtcggtc atcatggcgc
     5761 gcagcgccgg gatgtcggag cggtcccagg catcctgcag ggtgatgaag ttgcgcttgg
     5821 cggcctcggt gaagccggcg atgtcgaaat cggccggcac accccagccc tgggcgccac
     5881 gcagcccgga gccgatgatc gatccccccg aggcgccacc ggcgtcaaac ggcgccgcct
     5941 gaccttccca gggacgggcc gaggcgtcgt tgcccacctt ggtcgggttg tactggcgcg
     6001 gtgtgatcgg ctgcgcggca ccggcgccct ggtaggccat gccgggcccg ccggcggccg
     6061 cggcaccacg cccgcggcgc agcatgccga tcaccgccag caccaccatg cccagcaaca
     6121 ggatcagcag gacgttgccg aacgcctgac ccaggcccag cgcgtgggcc agccaggcca
     6181 ggcccagacc ggccgccaga ccgcccagca tgctgcccca gggtttgcgc gcggccgcac
     6241 cggccgcgcc tgccgccgcc gcaccgcccg cggcggcacc ggcggtggcg ctggactgcg
     6301 ccggcgccac cggcgtgccg gggccggccg cttcgcgctg cgtcacgttg ctggactggc
     6361 gcccgagcga cttgccaccg cccaggcgct tggcctcggc ctcgaagctg aacgcgccgg
     6421 tcagcaccac cgccagcagc agcgtgaaga aatgagaaac gagcttcttg aggttcatga
     6481 ctgaccggac tcccctgacg actgaaagtt ccacacggat atccacggct ttataaaggt
     6541 tatccgcatt tgatgccgac atgcaaggca acgaccccag ccgtcatgct gtgatagtcg
     6601 acatgaccga aaccgttggc cttcatcaac gccttgagtt cttcctggtt ggggtgcatg
     6661 cggatcgatt cggccaggta gcggtagctg tcggcgtcgc ctgccaccag ccgccccagc
     6721 cgcggcagca cctggaacga atacaggtcg taggccttgc gcagcggccg cgccacctgc
     6781 gaaaactcca gcaccagcag ccgaccgccc ggcttgagca cgcgcgccat ctcgcgcagg
     6841 gcctggtcct tgtgcgtcat gttgcgcaga ccgaaggcga cgctgaccac gtcgaaccgc
     6901 tcgtcgggaa aaggcaaggc ctcggcgtcg cacaccgtgg tgggcaggat caccccggca
     6961 tccagcaggc ggtcgcgccc ggtgcgcagc atggcttcgt tgatgtcggt gtgcaccacc
     7021 tgcccatgcg ggccgacccg gcgggcgaag gccagcgcca gatcgccggt gccgccggcg
     7081 atgtccagca cgctctgacc cggctgcagg ttggccacca tcacggcata ggccttccag
     7141 gcccggtgca ggccaccgga catgaggtcg ttcatgaggt cgtaccgcgc ggcgaccgaa
     7201 tcgaacacgc cgcgcacgcg gcgcgccttg tcctgttcgt caaccgactg gaaaccgaaa
     7261 tgcgtggtgg ccatggggcg ctgccgtggt ggtggatcgc tgcgggaaac ggcgcccacc
     7321 gctcgcgcgg ctgacgccct ggcgtcaagt gtaaggcgcg ccatgcccat aatggccgca
     7381 tgccacgccc cagccagctt ctgcatccgc agcgcgaacc cgcgcgtccc ctgcccgccg
     7441 ccaccgtgct gctgctgcgc gacacgcccg ccggcatcga agtgctgatg acgcgccgct
     7501 cgctgaacgc cagttttgcc cccggcgcct acgtgtttcc cggcggcggc atcgaccccc
     7561 aggacgccgc cgcccacggt caggcgacat gccgacccac gcagagccag gcccacctga
     7621 cccaggccat cgccgccatc cgcgaaagct tcgaggagtt gggggtgctg ctggcgcggc
     7681 gtgccgacgg cacgccggcc gacgcgagcg acgtggcggc gctcgaccgc cacgccccgc
     7741 tggcggccca gtgcgccgcg cgcggcctga cgctggcggc ggccgacgtc cacatgctgg
     7801 cgcactggat caccgaccgc gacctgccgc ggcgcttcga cgtgcccttt ctggtcgccc
     7861 gcatgccgga cggccagacc ccggtggccg acgacgccga gcagttcgaa cccgtgtggg
     7921 tgcgcccggc cgacgcgctg gcgcgccacg aggcggggca attcttcatc atcttcccca
     7981 ccatccgcac gctgcagcgc ctgcagcact accccgacgt cgccgccgtg ctggccgcct
     8041 gcgccagcga acggccgcta tgggccagct gcccgcgcgc cggctggctg gccggcaagg
     8101 aagcgcgcta catggagcac gagatgccct ttggcgaact ggcgctggtg acgcccgatg
     8161 gccagctgct gcatgcgctc gactggcagt ccgagcagcc ggtgccgctg ctcaagaacg
     8221 tgcagcgcct gacggcgccc aaccccggct tcatgaccgg cccgggcacc aacagctacc
     8281 tggtgggcga cccgcgcagc ggctacatcg ccatcgatcc cggcccggcc gacccggccc
     8341 atgtggagcg gctgtggcgt gccgccggcg ggcacatccg cgccatcgtc tgcacccact
     8401 cgcaccccga ccactcaccc ggcgccctgc ccctgcaggc cctgtgccag ccgccaccgc
     8461 ccatcctggg actgccttcg ctgcccacgg cgcgcgccca cagccagttc cagcccgacc
     8521 gcgcgctaca ggatggagag cgcctggcgc tgaccgggca agccccggac ggcgaaatca
     8581 ccacccatac gctgcgcgcg atccacaccc ctggccacgc cgccaaccat gtctgcctgc
     8641 tgctggaaga agacggcctg ctgttcagcg gcgaccacat cctgaacggc agcaccaccg
     8701 tcatcgaccc gccggatggg gacatgacgg cctacctgga atcgctggac aagctcgacg
     8761 ccgcctgtgc cgaggcccag gcgcacttca tcctgccggc ccacggccac gtgctgggcc
     8821 aggcgcgcga caccatcgcc catcttaagg cgcaccggct ggcgcgcgaa gccaaggtgc
     8881 tggcggtgat gaaggccgac ccccacggca cgctggacga ctgggtggcc aaggcctacg
     8941 acgacgtgcc cgagcgcgtc tggccggtgg ccaggcgttc gctgctggcg catgtggagc
     9001 gcctccgggc catggcgccg ggcaacgact gagcctattg atccggccct ttgaagtctc
     9061 acattcggtt cgggccgagc ttgtcgatgt cttcgtcatg ctcagggcga acggtgacga
     9121 tttcatgggg ccggatccat ggccacggca gatgaccgct tgtcaacgtc gggcaggccg
     9181 cgcccatgcg cgtcaagggc cttcacctgt ggggtgagca cgccacgcac ccacccgcgg
     9241 cgcggcgcca ggacaccaac gggcggacac gaagggcgcg aaacaggcat cgggctgttt
     9301 cgcgcgcttg gcgtgctttc gcgtcctccg cgtccggatg tccgcctgcc gggcgcaaaa
     9361 aatgtgtgaa gcccaccgat acgccggatt ctgtgcggcg cggttgcccg cgccgtgacc
     9421 gccatcaatc tgggccgccg gtcgcccgtg cggctcggtg ccacctaccc gccagctccg
     9481 cgggtcacgt caacgctggc ctacttggtg ttgctgcgcg cagagattgc ccgtttcacc
     9541 cgcactcaag cggctcgtct ctgttgctct gatcctcacc tcacggtgga caggtgttac
     9601 ctgctgcgct gccctgtgca gtccggacgt tcctccagcg cgccctttcg ggccttgcgc
     9661 cagcggcggt ctggcgggct tcacggccgt gattatccgc ccagcgggcg ccggctgcac
     9721 gcggccgccg ttcactccag catggccagg atggtgtagg cgatcagcag cagcgcgccg
     9781 gccgtgccca gcaccatcag gcccaggccc cagcgcgatt cgcgcgtggc aaagccgatc
     9841 agcatcatca ggcagctgac gcccagcatc agcatggtgg agtcgaacag ggtcatgggg
     9901 gcacgtggtg acagccggaa agtgagccat tgtgacctgc cgcgcggcgg ggcatatcga
     9961 tatcacgcca ggtgggatca aggctcgcac aataggtgcc cctgcccatc accgaccgga
    10021 gagacacacc atgcccgccg acaacgtcct gcaaaccatc ggccgcacgc cgcacgtgcg
    10081 catccgccgc ctgttcggcc ccgaggcccg ggtgtgggtc aagtgcgagc gcgccaaccc
    10141 gggtggctcg atcaaggatc gcatcgccct ggccatgatc gaggcggccg agcaaagcgg
    10201 ccagctgcag cccggcagca ccatcatcga gcccaccagc ggcaacaccg gcgtcggcct
    10261 ggcgatggtg gcggccgtca agggctaccg cctggtgctg gtgatgcccg acagcatgag
    10321 cgtcgagcgg cgccggctga tgctggccta cggtgccagc ttcgacctga cgccgcgcga
    10381 ccagggcatg aagggcgcca tcgcccgcgc cgaggagctg gccgcgcagc tgcgcgccgc
    10441 cggccagggg gcctggatcc cgcagcagtt cgacaacccg gccaacgcgg cggtgcacga
    10501 acagaccacg gcgcaggaaa tcctggccga ctttcccgac gggctggacg cgctgatcac
    10561 cggcgtcggc accggcggcc acctgacggg ctgcgcgcgc gtgctgaagg cgcgctggcc
    10621 gcagctgaag gtgtttgccg tcgagcccga ccagtcgccc gtgatcagcg gcggcgcgcc
    10681 cggcccgcac ccgatccagg gcctgggcgc cggcttcatc ccgaagaacc tggacaccgc
    10741 gctgctggac ggcgtgatcc aggtcggcgc cgaagccgcg cgcgactacg gccgtcgctg
    10801 cgcgcgcgag gaagggctgc tggtgggcat cagcagcggc gccgcgctgg cggccatcgc
    10861 acagaagctg cccgagctgc cggccggcag ccgcatcctg ggcttcaact acgacaccgg
    10921 tgaacgctac ctgtcggtgg acggctatct gccaacctga ggcggcggcg ccaccgaccc
    10981 gaccggcata gaatgcccgc cggcgcgccg cgccgccaca gcctctggac agacaccatg
    11041 aaccgctgct cgaacgttct ccgaactacg ttttttatag ctgccagcgc ttgcctggta
    11101 agcgctggac tcggttttca tgccgaagtc cgggcggaac ggggctttcc gctcaagacc
    11161 gagcgggccg agatgaagtt catcgagctg cccgatgtgc tgctcaacgg caagcccgag
    11221 cggctggcgc acgcggtgcg cgtgcgcgac gagcgcaacg ccatcgtgat gccgggtgtg
    11281 gtcaacggtc gcaccgccgt cgtcaactac ctgcgcgatg cccgcggcca ggtgcgcgag
    11341 gtgtggatcc tgtcgccgca ggaagcggcg ctgccgctca agccggtgcc gaccacgcgg
    11401 cggccggcgc ccagcgccga cgccaccgcg tcctacaccc attgaccctg acgcggcggc
    11461 gtccacgcgc cgcctcgtcg tcggccgcgg cccggcggcc agcgccctcg ccccctcttc
    11521 ccggagacac cgcatgtccc gcaaagtttt catcaagacc ttcggctgcc agatgaatga
    11581 gtacgactcg gccaagatgg ccgacgtgct gggcgccgcc cagggttacg agcccaccga
    11641 cgacccggag caggccgacc tgatcctgtt caacacctgc tcggtgcgcg agaaggcgca
    11701 ggagaaggtg ttttccgacc tcggccgcgt caagcacctg aaggccaggg gcacgctgat
    11761 cggcgtcggc ggctgcgtgg ccagccagga gggcgaagcc atcatcgccc gcgcgcccta
    11821 cgtggacgtg gtgttcggcc cgcagaccct gcaccgcctg cccgagctgc tggccgagcg
    11881 ccagaagcag aacgcgccgc aggtcgacat ccgcttcccc gagatcgaga agttcgacca
    11941 cctgccgccg gcgcgcgtcg atggcgccag cgccttcgtc tcgatcatgg agggctgctc
    12001 caagtactgc agctactgcg tggtgcccta cacgcgcggc gaggagttca gccgcccgct
    12061 cgacgacgtg ctgaccgaga tcgccggcct ggccgaccag ggcgtcaagg aagtcaccct
    12121 gctgggccag aacgtcaacg cctaccgcgg gccgatgggc gacagcagcg agatcgccga
    12181 ctttgccctg ctgatcgagg tcgtggccga gatccccggc atcgagcgca tccgctacac
    12241 caccagccac cccaacgaat tcaccccgcg cctgatcgag gtgtacggca aggtgcccaa
    12301 gctggtcagc cacctgcacc tgccggtgca gcacggcagc gaccgcatcc tgatggccat
    12361 gaagcgcggc tacaccgcca tggaatacaa gagcatcgtc cgcaagctgc gcgcggtgcg
    12421 gccggacctc agcctgtcga gcgacttcat cgtcggcttt ccgggcgaga ccgagcagga
    12481 cttcgacaag ctgatgaagc tgatcgagga cgtgggctac gacgcctcgt tcagcttcat
    12541 cttcagcccg cgccccggca cgccggcggc caacctgcac gacgacacac cgcacgccgt
    12601 caagctggcg cgcctgcagc acctgcaggc ggtggtggag cagaacgtgc gcgccatcag
    12661 cgccagccgc gtcggcacgc gccagcgcat cctggtcgaa gggccgtcgc ggcgcaaccc
    12721 gaacgagctg atgggtcgca ccgagtgcaa ccgcatcgtc aacttcgacg ccggcgcaca
    12781 gggcgcgcgc ctggtcggcc agatgatcga ggtcgacatc accgaagccc tgccgcactc
    12841 gctgcgcgcg cgcctggcga gcgccgcagg ctgagcggca gcgccggccg gcgggtgtta
    12901 cattgcgcgt cggcgcctga gggtgcccgc caccgtacca cccgccgaca ccgcgcccgc
    12961 ccgccccgat gccgccgccc cgcctcgaac tgacgcagat ccgcaagcgc tacccgggcg
    13021 tgctggccaa cgacgacgtg tcgctgacgg tgcagccggg cgaaatccac gccgtgctgg
    13081 gcgagaacgg cgccggcaag agcacgctga tgaagatcat ctacggcgcc gtcaagccgg
    13141 acgccggcga gctgcgcttt gacggtcagc cggtgcgcat cgccaatccg cagcaggcgc
    13201 gccggctggg catcgccatg gtgttccagc atttcagcct gttcgaaacc ctgagcgtgg
    13261 ccgagaacgt gtggctgggt ctggacaagg cgctgccgct ggccgaggtg acgcgccgca
    13321 tcgaccagac cgccgccgcc tacgggctgg agatcgaccc ggcgcgcccg gtgcacacgc
    13381 tgagcgtggg cgagatgcag cgcgtggaga tcatccgcgc cctgctgacc gagccgcgcc
    13441 tgctgatcct ggacgaaccc acctcggtgc tgacgccgca ggcggtggac aagctgttcg
    13501 tcaccctgcg ccggctggcc ggcgagggct gctccatcct ctacatcagc cacaagctgc
    13561 acgagatccg cgccctgtgc agcgcctgca ccgtgatgcg cgccggccgc gtgaccggcg
    13621 tgtgcgaccc gcgccaggag agcaacgcct cgctgtcgcg cctgatgatc ggtgccgagc
    13681 cgcccgagct ggcgcaccgc ccgcaggccc tgggcgcgcg ggtgctgcag gtcgagcggc
    13741 tggatctgaa gcgcgagtcg cccttcggcg tcgatctggt ggacctgtcg ctgcaggtgc
    13801 gggcgggcga ggtggtgggc attgccgggg tctcgggcaa tggtcagcgc gaactgctgt
    13861 ttgcgctttc cggcgaggac cggcgcgcgc cagcagcttc tatattgata gcagaccagg
    13921 ctgccgggcg gctctcgccg agccggcgcc gcgcgcttgg cctgcacttc gtgccggaag
    13981 agcgcctggg ccgcggcgcg gtgcccgaca tgagcctggc gcacaacctg ctgctgacgc
    14041 ggcgcgaggc cgtgggcccg ctgggctggc tgcgcaccgg cacgctgcgc gcgcaggcgc
    14101 aggccatcat cgagcgcttt cgcgtcaagg ccgccgggcc gcaggcgccg gcgcgctcgc
    14161 tgtcgggcgg caacctgcag aagttcatca tggggcgcga gatcgacgcc cggccgcggc
    14221 tgctgatcgt ttcgcagccg acctggggcg tcgacgtcgg cgcggcggcg cagatccgcg
    14281 gcgagctgct ggcgctgcgc gacgccggct gcgcggtgct ggtggtgagc gaggagctgg
    14341 acgagttgtt cgaggtgtgc gaccgcctgc acgtgatcgc caaagggcgg ctgtcgccgt
    14401 ccatcgcgcg ccaggacgcg acggtggagc gcatcggcga atggatgagc gggctgtggc
    14461 atggcgacgt gcagcagcac ctggcacaga tggaggccgg ccatgtggcg gcttgaaccg
    14521 cgccccgcgc cttcgcgcgc ctggacctgg ggctcgccac tgctggcgct ggccctcatt
    14581 gtgctgatcg gggtggcgct gttcaccgcg ctgggcaagg acccggtgcg cgggctgctg
    14641 gtgttcttct gggagccgat caagtccggc tacgcgctgg gcgagctgag cgtcaaggcc
    14701 acgccgctgc tgctgatcgc gctcgggctg gcggtgtgct ttcgctccaa cgtctggaac
    14761 atcggcgccg aggggcagtt catcgtcggc gccatcgccg ccggcggggt ggcgctgctg
    14821 gccgacaagg acagcgggcg ctggatcgtc ggcgccgtgc tgctggccgg ggtgctgggc
    14881 ggcatggcct gggccgccgt cacggcgttc ctgcgcgacc gcttcaacgc cggcgagatt
    14941 ctggtcagcc tgatgctggt gtacgtcgcc atccagtggc tgggctacct ggtgtatggg
    15001 ccctggaaag acccggccgg ctacaacttt ccgcagacgc gcagcttcga ggcggtggcg
    15061 caggtgccgc gcctgctgaa cggctcgcgc gcgcacatcg gcgtgctgat cgcgctggcc
    15121 ggtgcggcgc tgctgtgggt gtacctgttt cgcacccgca gcggctttgc ccagcaggtg
    15181 ggcggactgg cgccggcggc ggcgcgctac gccgggttct cgtcgcgccg cgcgctgtgg
    15241 acggcgctgc tggtgtcggg cggcaccgcc gggctggccg gcgcgctgga ggtggccggg
    15301 ccgctgcgcc agctcacccc gcacgtgccg gcgggctacg gctttgccgc catcatcgtc
    15361 gccttcgtcg gccggctgca cccgttcggc atggtgctgg cggcgatcct gatgagcatg
    15421 ttctacatcg gtggcgagct ggcgcagtcg cgcctggggc tgcccaagtc gctcaccggc
    15481 gtgttccagg gcctgctgct gttcgccctg ctggcctgcg acgtgctggt caaccaccgc
    15541 ctgcgccgcc gcggggggag ccactgatgg aagcctacgc gctgctgctg gcggcgacgc
    15601 tgaacgccgg caccgtgctg gccatcgccg cgctggggct gctgatcaac gaaaaggccg
    15661 gcatcgtcaa cctgggcgcc gaaggcatca tgctgtgcgc cgccgtggcc ggctttgccg
    15721 ccgtggtgca cagcggcagc gacgggctcg gctttgccgc cggcatggcc gccggggcgc
    15781 tgctggcggc caaattcggc tggctggtga tctggctggg caccaaccag tacgccaccg
    15841 ggctggcgct gtcgctgttt ggggtcggct tctcggcctt cgtcggcatc ggctacgtgc
    15901 aggcgcggct gccggagcgg ccgcgcttca acggcctggg cctgggcgat ctgccgctca
    15961 tcgggccggc gctgttccgg catcacccgc tggtgtatgg cgccatggcg ctggcgctgg
    16021 ggctgatctg gtttctctac cgcacgcgcg gcgggctggt gctgcgctcg gtcggcgagt
    16081 cgccgcaggc ggcgcacgcg ctgggctatc cggtgcggcg catccgcctg ctggcggtgg
    16141 tggccggcgg cgcgctgtgc gggctggccg gcgcctacct gtcggtgatc tacaccccgc
    16201 tgtgggtgga aggcatggtg gccggcaagg gctggatcgc gctggcgctg accaccttcg
    16261 ccacctggcg gccggcgcgg gtgctgctgg gcgcctacct gttcggcggc gtgaccatgt
    16321 tgcagttcca tctgcagggt cagggcgtgc agttgcccag ccagttcctg accatgctgc
    16381 cctacctggc gaccatcgtg gtgctggcgc tgatttcgcg caacccgacc tggatccgcg
    16441 tcaacatgcc ggcctcgctg ggcaaaccct tccacccggg ggcgtgatgg ccacaggcgg
    16501 cggcgccgac acccggaacc ggtggcgccg cccgcgcgtt ccatccgcaa actatcaaac
    16561 tcatagctgc cggcgcttgt ctgacgcgcg ccagaggtgt tttttgccca tgatcgtggg
    16621 ccgcagccgg gcggccggcg gggcggcgcg tcccgcataa tcgaccactt gttctttttt
    16681 tcatccaccc tcaggaggtt ctcccgtgct cgatccgaac aaacgctccc tgctgaaact
    16741 ggccgccctg gccgccgccg ccagcgcgct ggtggcgtgc agcaagaagg aggagcccgc
    16801 gcccacggcc gctgcgccgg ccgcaccggc ggccgccccg gcgcccgcca agcccgaacc
    16861 cctgaaggtg tcgtttgcct acatcggccc ggtgggcgac ggcggctgga ccttcgcgca
    16921 cgacaacgcg cgcaaggcgg tcgagaagga gttcggcgac cgcgtggtca ccagctacgt
    16981 cgagagcgtg cccgagagcg ccgacgccga gcgcgtgatc cgcgacatgg tgggccaggg
    17041 aaacaagctg atcttcggca ccaccttcgg ctacatggag tcgatggtca aggtcgcggg
    17101 cgaccatccg gacgtcaagt tcgagcacgc caccggctac aagaccgcgc ccaacctgcg
    17161 cacctacgac agccgcacct atgagggcgc ctacatggcg ggcgtgatcg ccggccgcat
    17221 gacccagtcc aacgtgctgg gtgtggtcgg ttcggtgccg atcccggagg tgctgcgcaa
    17281 cctcaacagc ttcacgctcg gcgcgcagag caccaacccg aagatcacca ccaaggtggt
    17341 gtgggtcaac gactggttca acccgcccaa ggaaaccgaa gccgccaccg ccttgctgaa
    17401 cgccggcgcc gacgtgctgt tccagaacac cgattcgccg gccgtgctca agaccgcgca
    17461 ggaaaagggc aagcgcgcct tcggctggga cagcgacatg accgcctacg gtcccaaggc
    17521 gcacctgggc tcggccatca tcaactgggc gccctactac atcaaggcgg tgggcgaggc
    17581 gctggaaggc aagtggagca ccggccaggc ctggtggggc gtgaaggaag gcgcgatcga
    17641 cctggtgtcg ctggccgagg acgtgccggc cgacgccaag gccaaagtcg aagaggtcaa
    17701 ggccgggctg aaggccggca ccttccacat ctggaaaggc ccgatcagcg acaacaccgg
    17761 caagcaggtg ctggcggccg accaggtggc ggacgacaag ttcctcaccg gcatcaactt
    17821 ctacgtcaag ggcgtggaag gcaaggtgcc gggcggcgag aaaaaataag ccggcccccg
    17881 cgccatgcgc agccgcgccc ccgggcgcgg cttttttgtg gcccgcaggg cagcgacccg
    17941 gcccctgaat gctatcgata gcatagctgc tggcgcaatc tccgtctgcg ccatcgacga
    18001 attcttgcca gattccgtca tcgacccgca gcgaggtcat gcgtcatgcg tcacgacgcg
    18061 cagaccccga tccgtccgcc gccgcaagct gacgcagcgc ctgctcgaac acctcgggcg
    18121 gctggccgcc gctgatcagg tggcgctggt tgaggatcac cgccggcacc gcatggatgc
    18181 cggcgtcctg ccactggcgc tcggcctggc gcacctcggc ggcaaactcg tcgcccgcca
    18241 gaatctgcgc cgcccgcgcc tcgtccagcc ccgcctggcg cacggcggcc agcaggaccg
    18301 gggcctggtc gacccgctcg gcgcgtccat ggtaggcctg cagcagcgcg cgcttgagcg
    18361 ccagctgggc gtccgccccc tgccgaccgg cccagtgcag caggcggtgc ccatcgaagg
    18421 tgttgacgac gcgcccgcgc ccttcggggt ggaaggcaaa gcccagcgcc gcgccgcgct
    18481 cggcgatgac ggcgcgcatc tgcgcctgct gcgccggcgt ggagccgtac ttgcgagtca
    18541 ggtgctcgcc gaggtcctca ccctcgggcg gcatggcggg gttgagctcg aaaggctgaa
    18601 cacgcagcct cacctgcacc gcgtcggcgc agcgcgccag cgcctgctgc agcgacgcca
    18661 ggccgaccgc gcaccaggga caggcgatgt cggagacgaa gtcgatgtcg atcggtgtgg
    18721 ccatgcaaac cctccaagtg ctactaacta aatagctgcc ggcgctttgc cggtgcgcgt
    18781 cagggccatg aatcgcttaa atccgaccgc tggcccattc gtgcgccagc gctgcgcctg
    18841 tggcgcgtcg gtggtgccgg cttcgcgtcc gcgacgtcct tcacgtccgg ctgccggagc
    18901 gtgccggcag gccatcaatc ccccagcgcg cgccgcagcg ccgcgtcgaa ggcggcgggc
    18961 gcctcgaact gcacccagtg gccggcgccc tcgatcagct gcaggccacg gaagtcgggc
    19021 gcgctgcgcg cgaacgcgcc ctccagcgcg tggatgtgct ggcgatagat ggcatcgtgc
    19081 acgccgtaga tggcatgcac cgggcaggcc acctgcggca ggcggcgcgc caggatgtcg
    19141 gtatgcgcca gccgccggcg cggcaggcgg tcgcgctgca cgttggcgat gtgcagttgc
    19201 agcgccaggc cgtcgatcag gcccggatcg tgcagcatca gcaccgccag gttgtggcga
    19261 tgcacctcgg cctgcgccgc cggcgacggc aggtggcgcc aggcgcgcag ctcgaactgc
    19321 cgcccgggca ccacgcccat ggccggcgcc cccaccagca ccagcctgcg cgccagcgcc
    19381 gggtgttcgg ccagcagcag ggcggcggtc aggccgccga gcgaaaagcc caccaggtcg
    19441 accgcctcgc ccggctgcag ctgccgcagg ccgttggcca gcggctcgac catggcatcg
    19501 gcatcgctgc cctgcgtggg tggggccgaa tcgccaaagc ccggcaggtc ggccgccagc
    19561 acctgacgcc cggcatcgag cagcggcgcg atgttcttca cccagtgggt ccatgagccg
    19621 ctgccgccat gcaacagcac caccggcggc tggcccgcgc gccgcgtacc ccagcggtgc
    19681 cagaccagct cgccctgccc gcccggcgtg gtcaggcgct ggccgctggc caggcgcaag
    19741 cgctcggccg gcggcagcgc ggcggggaag gcggcggcgt ccagcggggt cgggccggcg
    19801 ctcgcggcgc gctggcctgg gcctgggtca ggggtgcggt tcattcgatg cccgccgacc
    19861 agcgttcgcc ccagtcggcg cgctgctgct ggtccagcgc acgccggtcg cgcttggtgg
    19921 gccggccctg ggcgatgctc agcgccggct cgggcgccag acggcgcgcg ctggcggcct
    19981 gctggcgcgc ggcgacgctg gccggcgttt cctcgtacag ctgctgcgcc accggggccg
    20041 ggccgcgctg agggctgagc gccctgacca ccacctcgcg ctgcaccacc ccctggcgca
    20101 gactgagcac atcacccggc ctgagctcgc gcgccggctt gaccgtctgc cccgccacct
    20161 gcacgcggcc gcgctcgatc tcctgcaccg ccagcgcgcg tgtcttgtag aagcgcgcgg
    20221 cccacagcca tttgtcgatg cgttgtcgtt ccatggggtc ggccatgggc tcgattctgc
    20281 ccggctttgc ctgcggcgga cgcgggcggg gatactcacg ggtgatgcca caaccctccc
    20341 atcccccggg cctgagcggc gacgaagcgc gccggcgcct ggccgagcat gggcccaaca
    20401 ccatcgacga gcgcgagcgg cgcagcctgg ctggcacgct gcgcgagatt gcgcgcgagc
    20461 cgatgtttct gttgctgctg gtggcggcgg ccatctacct gctggtgggc gagccgggcg
    20521 aaggcctgct gctgggcggc tttgcgctgc tgacggtcgg cctggtgatc ttccagcagc
    20581 ggcgcggcga gcgcgcgctg gacgcgttgc gcgagctggc ggcgccgcag gcccaggtgc
    20641 tgcgcgaggg ccgcgtgcag cgcatcgcgg tgcgtgagat cgtgcccggc gactggatcg
    20701 tgctgggcga aggcgagcgc gtggccgccg acgccgtgct gcgccaggcc caggcgctga
    20761 cggtggacga gtcgctgctg accggcgaat cggtgccggt gcgcaagtgc gccagccacg
    20821 ctccgctggt cgaggcggcg accgatcggc ccggcggcga cgatctgccg caggtgtttg
    20881 ccggcaccct gatcaccgcc ggccacggcc tggccgaggt ctgcgccacc ggtcgccaga
    20941 cgcagatggg acgcatcggt gcggcgctgg cgacgatcga gccggccgcc acgccgctgc
    21001 agcgcagcct gcaccgactg gcgcgctggt tcggcgcgct ggcgctgacc ggcagcgcgc
    21061 tgctggtgct gtggcacggg ctgcgggggg gcgactgggt gcagggcctg ctgtccggca
    21121 tcgcactggg catggccatg ctgccggaag agttcccgat ggcgctggcg gtgttcctgg
    21181 cgctgggcag ctggcgactg gcgcgcatcc aggtgctggc gcgccagccc gccgtggtcg
    21241 aggcgctggg ggccaccacc ctgctgtgtg tcgacaagac cggcaccctg accgaaaacc
    21301 gcatggcgct gcgccggctg gtgacgcagg aggtcgacgt cacggtgcag gccggcgcgc
    21361 ccctgcccgc gtcggtgcac cggctgctgg catgcgccat gctggcgtcg cggcgcggca
    21421 ccagcgaccc gatggatcgc gccctgctgc tgcacggcga ccaggcgctg gccggcaccg
    21481 agcacctgca ccccgactgg cagctggagc gcgagtacgc cctcaccccg gcactgcccg
    21541 ccatgacgca gacctggctg gacgacgccg gtcggcgcca tgtggccgcc aagggcgcgc
    21601 ccgaggcggt gatcgagctg tgccacctgc cacccgcggc ggcggcccgc ctgctggcgc
    21661 aggtggcggc gctggccgcc gccggcttgc gcgtgctggc cgtggccgag ggccagggca
    21721 gcacggccga gaccgcgccc gacccgcacg actacgacct gcgcctgctc gggctgctgg
    21781 ggttcgagga cccgctgcgt gccagcgtgc cggcggcggt ggcgcaggcg cacggcgccg
    21841 gcatcgccgt ggccatgatc accggcgacc acgccgccac ggcgctggcg atcgcacgcc
    21901 aggccggcat cgacacggcg gccggcgcgc tcaccggcgc gcagctcgcc gccctcgacg
    21961 atgccgcgct gtcggccgcg gtgcgccggg tgcgcgtgta cgcgcgcgtg ctgcccgagc
    22021 agaagctgcg cctggtcgag gccatgcagc gcaacggcga gaccgtggcc atgaccggcg
    22081 acggcgtcaa cgacgcgccg gcgctgaagg ccgcgcacat cggcatcgcc atgggcgggc
    22141 gcggcaccga cgtggcgcgc gaagcggccg gcctggtgct gctggacgaa gacttcagcc
    22201 gcatcgtcgg cggcgtgcgc atgggccggc gcatcttcga caacctgcgc aaggcgatgg
    22261 tctacatcgc cgccatccac gtgccgatcg ccggcttggc gctgctgccg gtgttgctgg
    22321 gcctgccgcc gctgttgctg ccggcgcacg tggtgctgac cgagatggtg atcgacccga
    22381 tctgctcact ggcgttcgag ggcgcgccgg aagacccgcg cctgatgcag cgcccaccgc
    22441 gccgggccgc cgaaggcctg gtcggccggt cgctgctggc gcaggcggcg gtgcagggtg
    22501 cgagcgtgct ggccgccacg ctggcgatct acctgcaggc gctgcacggc ggccagagcg
    22561 ccgacgtggc gcgcacgctg gccgtgatcg gcctgatggc gggcaacctg ctgctggtgg
    22621 ccgtcaacct gagcgcgggc ctgggctggc gcgccctgct ggcccgcggc gcgcgcgcct
    22681 tctgggccgt ggcggccatc gccacggcgg tgctgggcct ggcgctggcg ctgccgcagg
    22741 cgcgcgcgct gctgcgcttc gggccggtgt cgctggcaca ggtcggcctg gcgctgcttg
    22801 ccgtggcgct ggccgtggtc gtgggcgcgc ggttcggccg ggcgctggag cgcagcacgg
    22861 cctgacggcg ccgcgcgcgc ttcggcccgc gcgctaccat gcgcgcatgc ctgccacgct
    22921 gtcgcccgcc ccgtccgcac tgccgcgcga cgccagccgc atcctggagc tggcggcgcg
    22981 ctcgctgttc gagctgttcg aagacgccag cgaaggcatg ctgctggtcg accgcgacgc
    23041 ccgtgtggtg tggatcaacg acccctaccg gcgctttttg ccggcactcg gattcgagcg
    23101 cgaggaagac ttcgtcggcc acccggtgtc ggacgtggtg cagaacaccc agatgcacca
    23161 ggtgctgcgc accggcaagc cgatcctgat cgacctgctg agcaaccgcg ccggcacctt
    23221 cgccgtcagc cgcatcccgt tgcgcgacgc ggcgggcgaa gtcatcggcg cgctcggcat
    23281 cgtgctgttc gaccagacct cgcacagtct gcagcccctg ctggcgaagt tcgcgcgcct
    23341 gcagcaggaa ctcgacgacg cccagcgcga gctggcggcg cagcgccgcc agcagggcga
    23401 tggccagcgc cgcgcccgct acacgctggc cagcttcgtc ggcagcagcc cggcggccag
    23461 cgaggtcaag cggcaggcgc gccgcgccgc gcagtccagc agcccggtgc tgctgctggg
    23521 cgagaccggc accggcaagg aactgctggc gcacggcatc cacgccgcct cgccgcgcgc
    23581 cggcgggccg ttcgtcagcg tcaatatcgc cgccgtgccc gacaccttgc tggaagccga
    23641 gttcttcggc gtcgcccccg gcgcctacac cggcgccgac cgcaaggggc gcgaaggcaa
    23701 gttcaggctg gccgacggcg gcacgctgtt tctcgacgag atcggcgaca tgccgcccgg
    23761 cctgcaggcc aagctgctgc gcgcgctgca ggatggcgag atcgaaccgc tgggcagcaa
    23821 ccggctggtg ccgttcgacg cccgcatcat cgccgccacc agccgcgacc tgccgcagct
    23881 ggtgcgggcc ggccagtttc gcgaggacct gtactaccgc ctgaacgtac tgccgatcca
    23941 ggtgccgccg ctgcgcacgc ggcgcggcga cattccggcg ttgatcgagg tgctggccga
    24001 agacatgctg caccgcggca gccagccgcc ggagctgacc ccggccgccg tggcgctgct
    24061 ggcggcccag cactggcgcg gcaacatccg ggaactgcgc aatgtgctgg agcaggccgc
    24121 gctgcgcgcc gacgccgggg cgctcgacgc cgaaccgatg gaagcgctgc tgcgccagtc
    24181 cggcctgagc ctggtgcaac ccgccctgcc gcccgagccg ccaccccagg acgccgccgc
    24241 gctgctgcgt ccgctgggcg aacaggtggc cgaactggag cgccgcgcca ttgcggccgc
    24301 gctgcaggcc acccacggca acaagctggc cgcggcgcgg ctgctgggca tctcgcgcgc
    24361 cacgctgtac gggcgcctgc tcgaacccgg cgacgctccg cagaaaccct gatctaaatt
    24421 cagtcatttg tctgaatatc agacaaagaa tttgtccatg attcagacac tttgaagctt
    24481 gcggccaagc agagcgccat gaatcaatga cttggcagct ggcacggctt gtgcatggtg
    24541 gatggtgaca tcgtcacgtc cacaggagac cctcatgcaa cgccgctcgc tcattgccct
    24601 cgcttcgttc gccgtgctga ccggtgcgcc ggccttcgcc gccggcgaca tcaagatcgc
    24661 ccacgtctac agcaagaccg gcccgctgga agcctacggc aagcagaccc agaccggcct
    24721 gatgatgggc ctgcagtacg ccaccggcgg caccatgcag gtgaacggca ggaagatcac
    24781 cgtgatcgag cgcgacgacc agggcaagcc cgacctgggc aagagcctgc tggccgccgc
    24841 ctacgccgac gacaaggtgg acctggcggt cggcccaacc tcgtcgggcg tggcgctcgc
    24901 catgctgccg gtggccgagg aatacaagaa gattctgctg gtcgaacccg ccgtggccga
    24961 cgccatcacc ggcgacaagt ggaacaagta catcttccgc accggccgca acagcagcca
    25021 ggacgccatc agcaacgccg tggccatgga caaggacggt gtttcggtgg ccacgctggc
    25081 gcaggactac gccttcggcc gcgacggcgt gaaggcgttc aaggacgccc tgaaaaaagc
    25141 caggatcgtg cacgaggaat acctgccgca gaacaccacc gacttcaccg ccggcatcca
    25201 gcgcctgatc gacgccctga aggacaagcc cggccgcaag gcgatcgagg tgatctgggc
    25261 cggcgccacg ccgcccttca acgccctggc cgcggccgac ttgaaaaagc gctacggcat
    25321 cgaggtgttc accggcggca acatcctgcc ggccatggcc agctacaaga acttccccgg
    25381 catggaaggc gccacctact actacttcgg catccccaag aacccggtga acgaagccct
    25441 ggtggcggcg cactacaagg aattcaaagc gccgcccgac ttcttcacgg ccggcggctt
    25501 ctccgccgcc atggccatcg tcaccgcgct caaggccacc ggtggcgaca ccgcgaccaa
    25561 caagctcatc aagaccatgg aaggcatgag cttcgacacg cccaagggca agatgacctt
    25621 ccgcaaggac gaccaccagg ccatgcagag catgtaccac ttccgcatca aggccgatcc
    25681 ggccttcacc tggggcgtgc ccgagctggt gaaggaaatc aagccggaag aaatgcaggt
    25741 gccgatccgc aacaagcgct gatggcgtcg atggcgcatg acgcatgacg catgacgggc
    25801 gtgtcgtcgg tgcgatgcgc ccggcacccc gccatgggtc atccatgccg cgccagcggc
    25861 gaggtcatat cgtcattggc aacacaccga catgctagag accgaagatc tgacgatccg
    25921 ctttggcggc catgtggccg tcaacgccgt gacgtgccgc ttcgaacccg gcacgctgac
    25981 ggccatcgtc gggcccaatg gcgcgggcaa gaccacctac ttcaacctga tctcgggcca
    26041 gctcaaggcc agcgccgggc gcgtgctgct gaacgggcgc gatctgtcgg cgctgccggc
    26101 gtcggcgcgc acccaggccg ggctggggcg ggcgtttcag ctcaccaacc tgtttcccaa
    26161 cctgtcggtg ctggagaacg tgcgcctggc ggtgcaggcg gcgcaccgca tcggcctgaa
    26221 cctgtggcgc atctggagcg accagcgcga gctgacgcag cgcgcgctgg agatcctgga
    26281 gtcggtcgcc ctgctcgacc gccagggcat gccggtggcc ggcctgccgc acggcgacca
    26341 gcgcaagctg gaagtggcgc tgctgatggc gctcgacccg caggtctaca tgttcgacga
    26401 gcccaccgcc ggcatgagcc acgacgaagc gccggtgatc ctggacctga tccggcgcct
    26461 gaagcaggac cgcagcaaga ccatcctgct ggtcgagcac aagatggacg tggtgcgcga
    26521 gctggccgac cgcatcatcg tgctgaccaa cggccagctg gtggccgacg gcgagccggc
    26581 cgaagtgatc gcctcgccgg tggtgcagga ggcatacctg gggctggcgc cccaggatgc
    26641 cttcggcagc accgacgctc gcccccatcc cggccttccc ccatccgggg gaaggagttt
    26701 gggagataca gcaccatgag tgaaaccctg ctcacgctcg aaggcgtgca cacccacatc
    26761 ggcgcttacc acatcctgca tggggtggat ctgcaggtgc cgcgcgggca gctgaccatg
    26821 ctgctgggac gcaatggcgc cggcaagacc accacactgc gcaccatcat gggcctgtgg
    26881 caggccagcg ccgggcgcat cggcttcaac ggccaggaca tcacccgcag gcacacgccg
    26941 cagatcgcac agctgggcat cgcctacgtg cccgagaaca tgggcatctt cgccgacctg
    27001 acggtgaagg agaacatggt gctggccgcg cgtggcgcac gcagcgcggc gcagatcgac
    27061 ggcgagcggc tggcctggat cttccggctg tttcccgcgg tggagaagtt ctggcaccac
    27121 ccggccggca agctgtcggg cgggcaaaag cagatgctgg cggtcagccg cgccatcgtc
    27181 gagccgcgcg agctgctgat cgtcgacgaa cccagcaagg ggctggcgcc ggccatcatc
    27241 aacaacatga tcgacgcctt ccagcagctc aaggccagcg gcgtgacgat cctgctggtg
    27301 gagcagaacc tgagcttcgc gcgccgcctg ggcgaccgcg tggcggtgat ggacaacggc
    27361 cgcatcgtgc acgccggcag catgcaggcg ctggccgagg acgccgcgct gcagcagcgg
    27421 ctgctggggc tgtcgctgtg acaaatacgc attcaattgg cctgtggcgc ttgctggacg
    27481 cgcgccagca gctattgttt ttgtagtctc cgaggcgcat gaaactcgac ttcgattacc
    27541 agccgctgct gctggtgccg gtgctggcgc tgctggcgct gccgctggtg ggctcgggct
    27601 cgacctggct gacgctcacc gtggccgggc tggccatggg catgatcatc ttcatcatcg
    27661 cctc
//
LOCUS       DAOQTZ010000025        27536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2045_length_27536_cov_13.028347, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000025 DAOQTZ010000000
VERSION     DAOQTZ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2045_length_27536_cov_13.028347"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1301
                     /locus_tag="PKN51_04715"
     CDS             147..1301
                     /locus_tag="PKN51_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT84434.1"
                     /translation="MLANKKALLAAAITFGCTAAWADINVGVTLSATGPAASLGIPEK
                     NTIALLPQTIGGQKINYIVLDDASDTTQTVTNTRKLITERNVDVIIGSSTTPTSLAMI
                     DVVAEAQTPMISLGASAAIIEPQDGKKRWAFKTPQNDIMMALAIAEHMAANGVKTVGF
                     IGFSDAYGEGWYRETVKALDLKKIKLVANERFSRTDTAVTGQVLKLIGAKPDAVLVAG
                     SGTPSALPQKTLKERGYTGKYYQTHGVANNDFLRVGGKDVEGTLLPAGPVLVADQLPA
                     ANPVRKSALEYINAYEGANGKGSVSTFGGHAWDAGQILKAAIPVALKGGQPGTPQFRN
                     ALRDALEGVKEVPGAHGIFNMSASDHLGLDQRARVMVKIENGRWKYQEVK"
     gene            1389..2417
                     /locus_tag="PKN51_04720"
     CDS             1389..2417
                     /locus_tag="PKN51_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT84435.1"
                     /translation="MDLQIAVMLVQDGIINGAIYALMALALVLVFSVTRVIFIPQGEF
                     VAYAALAMAALQAGQSPPLLWMLLGLAALVLIVELVRQTRGAEVDWRSTLFWAVLLPL
                     LAALMLLVLKPQAMLLRSLAVLLLVTPMGPLLYRLAYRPLADATVLILLIVSVALHLA
                     LVGLGLFFFGAEGSRTEAFSDARWDLLGLTVSGQSLVILGVTAALVVGMFLFFERTIV
                     GKALRATAINRVGARLMGIPTALSGDLSFALAALIGAVSGLLIAPVTTIYYDTGFLIG
                     LKGFVAAIVGGLASYPLALAGALLVGQLESFASFWASAFKEVLVFTLIIPVLWWRSLH
                     THHVEDEE"
     gene            2414..4222
                     /locus_tag="PKN51_04725"
     CDS             2414..4222
                     /locus_tag="PKN51_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HNT84436.1"
                     /translation="MKPMHLVLAFIVLLGAAWGFLPPFTVTLLSYIGLYALVAVGLVM
                     LTGVGGMTSFGQAAFVGLGAYATAWISLSPEAAQWLGALPKGAYPWVGLLLGLAVTAA
                     VAWVLGAVTLRLSGHYLPLGTIAWGLSLYYVFGNLEMLGGFTGLSGIAPLEVFGVSLA
                     SPRRLGLVIWAVLLLAIWGLHNLLDSREGRAIRALKSGRLMAESMGVDTARQRIKVFV
                     LAALLAALSGWLYVLLQRFVNPTPFALNIGIEYLFMAVVGGSGHLWGAVLGATLITLL
                     KEQLQDWLPRLLGSSGNFEIVVFGLLMLLVLQRAPNGLWPMLAGALQRFTGAAAPRRL
                     AAGAATLAQRQLPPAGEQILQADKVTKRFAGLLANNAVDLDVRAGEVHALIGPNGAGK
                     STFFNMISGVDDPTEGQVRLMGQVMQGQPSRAFAALGLGRTFQHVRLLGERSVLENVA
                     LGAHRRGRQGWLASMLRLDRAEEAAVLAEAMRQIERCGLAEVAHTPAAALPLGQQRIV
                     EIARALAGQPAILLLDEPAAGLRHLEKQGLATLLTQLRSEGLAILVVEHDMEFVMNLA
                     DRITVLEFGTVIAHGTPAEVQANPRVIAAYLGGDVL"
     gene            4219..4959
                     /locus_tag="PKN51_04730"
     CDS             4219..4959
                     /locus_tag="PKN51_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT84437.1"
                     /translation="MSAPLLSLPQFSVFYGPVQAIHGIELHVGEGEIVTVIGPNGAGK
                     TTLLNAIMGLLPSQGGLRLAGHSVARPTVETMVRHGVALVPEKRELFGEMSVEDNLLL
                     GGFALWRRGQRDQAARMKEIFGIFPRLEERRAQMASTLSGGERQMLAIGRALMSRPHL
                     LMLDEPSLGLAPLIVREVLHTVSALRRTGVSVLLVEQNARAALNVADRGYVLEMGEVA
                     VQGDAGELMHDQRVIDTYLGVGGARAAA"
     gene            5106..6650
                     /gene="paaZ"
                     /locus_tag="PKN51_04735"
                     /pseudo
     CDS             5106..6650
                     /gene="paaZ"
                     /locus_tag="PKN51_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
     gene            7399..7851
                     /locus_tag="PKN51_04740"
     CDS             7399..7851
                     /locus_tag="PKN51_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNT84438.1"
                     /translation="MSSTASGGFTKEMARNIFYGGSLFFILIFLGLVFHTEQRLPERT
                     NQAAMTDAVVRGKAVWEQNNCIGCHTLLGEGAYFAPELANVYQRRGGDASGAVFIKAW
                     MKAQPTGTPGRRQMPQFHLSEQQLDDLVEFLKWTGGINAEQWPPNIEG"
     gene            7871..9328
                     /locus_tag="PKN51_04745"
     CDS             7871..9328
                     /locus_tag="PKN51_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNT84439.1"
                     /translation="MSNNTILKYKSQAVAKPYFIAAIALFVGQILFGLVMGIQYIYGD
                     FLFPLIPFNVARMVHTNLLIVWLLFGFMGAAYYMVPEEAETELYSPRLALVMFWIFLV
                     AGALTIVGYLLVPYARLAEATGNNLLATMGREFLEQPLPTKVGIVIVALAMLFNISMT
                     YLKGRKTTITTVLLLGLWGLALLFLFAFVNPHNLVRDKMYWWWVVHLWVEGVWELILA
                     ALLAYVLIKTTGVDREVVDKWVYVIIAFALFTGILGTAHHYYFIGLPGYWHLVGNIFS
                     SLEPIPFFLMTMFAFRMVQRRRRDHPNQAAVLWAVGCAVLGFLGAGVWGFLHTLSFVN
                     YYTHGTQITASHGHLAFYGAYVLVVIALISYAMPTLRGRIANSYQAQALEMWSFWVMS
                     IGMGVMVLALTGAGIVQVWLQRMPESGAMPFMAAQDQVVFFYWMRLWSGVVFLIGLVM
                     YVWSFFVGGAPAEETLIERRGGLMPSPAQRRLRHD"
     gene            9458..10267
                     /locus_tag="PKN51_04750"
     CDS             9458..10267
                     /locus_tag="PKN51_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNT84440.1"
                     /translation="MDRTDTSPATTPFYAEQAGECRLFEHAFRQRLPVLLKGPTGCGK
                     TRFVEHMAARLGRPLVTVSCHDDLTAADLVGRFLIGDGNTVWNDGPLARAVRTGAICY
                     LDEVVEARKDTTVVLHPLADDRRVLPIERTGELLQATPEFMLVASYNPGYQNLLKNLK
                     PSTRQRFVALVFDYPRPEVEQRIIETEAGADAALSARLIKLATALRRLIDQDLEETVS
                     TRLLVVAARLIVSGLPPLTACRAAIVDALTDDLDTASALDQVVQAVLSHDA"
     gene            10307..12274
                     /locus_tag="PKN51_04755"
     CDS             10307..12274
                     /locus_tag="PKN51_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT84441.1"
                     /translation="MEEWVGERWHRFIWRAADRSFAHAAVRLEEVQRAVQLLFRAGGG
                     DPLVRVAPASAEKVGGPRSWLQKLAGQGERASIGTLDEQTLALPPVIAVFADAALNRD
                     LYLWLAAQAACHVATPAGWLADNLAATRRALACFPGLRTRYARLLQAHLAQRPAPERL
                     QGTPRELERAIQAALRASLDGVDAGRDTTPPAAREPCAPQDQGASLDGRARQPASVAP
                     VWLWLQAMQTAVGPSATAPEADAADGAPPRPQQQPTPQDPKRRRAQRTEDTSSKHGLV
                     MFFRAESILSWSEFVRVNRTADDEDNQDYSQVADDMQELALAPRKPGETLAARIKFDL
                     DLPSAAADDLPVGEGEPLPEWDYQRGLLLPEHCRAQRLRHRAASPDAIDSEAAGAVPA
                     LEKRLKTTSRQVRRRFEALRAAPRWQHGLTQGEELDLDAWVRHQVDRRDVAHRHVEAP
                     AVYARRVRSERSLSSLMLADLSLSTDAYARPGARVIDLVRDSLFVFGNALDAVGDPFE
                     MLGFSSVRRAVRIHELKRFGERWNDAARERVAAIKPGFYTRMGAAIRYGTQRLAARPE
                     RQRLLLILSDGKPNDLDHYEGRHGLEDTRHAVHAARQAGLVPFCVTIDQQANAYLPRL
                     FGTQGFALVHRPEQLARALTQVYVRLTAAVR"
     gene            12331..13029
                     /locus_tag="PKN51_04760"
     CDS             12331..13029
                     /locus_tag="PKN51_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT84442.1"
                     /translation="MDLKKFNLPRYLATLPLFSNMRDEELARLASGCQLRRMERGKLV
                     FRTGEPCQEFHVTVLGQVKLFAIAPNGTEKVIELCGPGHSFAEAVMFMDMPYMISAQT
                     LTDSLILTVGKAAVLGEIAANPDFSLRMLAGLARRLHGLVKDVEAYTLHSGVQRVIGY
                     LLGDRVSESEVASESITVSLPVSKAAIASRLSLTPEYFSRVLNELETAGLIQVDKRDI
                     RIPDTARLANHQPL"
     gene            complement(13045..14643)
                     /locus_tag="PKN51_04765"
     CDS             complement(13045..14643)
                     /locus_tag="PKN51_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNT84443.1"
                     /translation="MIFEVIKGTGLLVLALLGGPLAAQAPAPAAAPSIDAAALYQQHC
                     ASCHGEQRLGGMGPALLPESLARLRKPEALKVIRDGRAATQMLPFASALKADEIAALA
                     EWIYTPVTPPPRWSDADIAASREETAGWRDWPARPRWQADPMNLFVVVEGGDHHVSLL
                     DGDRFEVIHRFASRFALHGGPKFTPDGRYVYFGSRDGWITKYDLWNLKVVAEVRAGIN
                     MRNIAVSGDGKWVMAANYLPHTVVLFDAELKHRRTYAAHSLDGSQSSRVSAVYDAEPR
                     RSFVVALKDIPELWEISYDPKAEPIYDGFVHDYKMKEGLPKDGFLGVRRTVLDEPLDD
                     FFFSQNYAYALGASRPHADGSPSGQVVNLDVRRKIATLPIAGMPHLGSGITFDRGDTR
                     VLASPNLQDGVISVIDMKSWQLVKNIATPGPGFFMRSHEKTPYAWTDSMMSPKAKHIL
                     TIIDKRTLAPVARIEGTPGKTLAHIEFTKDGRYALASLWEDDGALIVYDAASFKEVKR
                     LPMRKPVGKYNVFNKITRSEGTSH"
     gene            complement(14695..15921)
                     /gene="nirJ"
                     /locus_tag="PKN51_04770"
     CDS             complement(14695..15921)
                     /gene="nirJ"
                     /locus_tag="PKN51_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HNT84444.1"
                     /translation="MFRISQFMHELARAEATGTYPEPARRGGPPGPVVIWNLIRRCNL
                     TCKHCYAFSADHDYPGELSTSQVFEVMDDLKAFKVPALILSGGEPLLRPDIFEVAERA
                     KALKFYVGLSTNGTLIDQPLAERIHATGFDYVGISLDGIGATHDKFRRLDGAFDRSLG
                     AVRHLQKLGTKVGLRFTMTELNAFDLPNLLRLMRDEGVDKFYFSHLNYAGRGNIHRGK
                     DAHHLATRQALDLLYDCAWQDAQAGGRHEYVTGNNDADGAYLLQWVQQHLPRWTEPVR
                     QRLIAWGGNSSGVNVANIDNLGDVHPDTMWWHHKLGNVKSRPFSAIWTDLSDPLMAGL
                     KARPRPVHGRCGACRHLAICGGNTRVRAQQVTGDAWAEDPGCYLTDEEIGVEPSATRI
                     ETTAFQSKKRVINVVH"
     gene            16054..16926
                     /gene="nadC"
                     /locus_tag="PKN51_04775"
     CDS             16054..16926
                     /gene="nadC"
                     /locus_tag="PKN51_04775"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNT84445.1"
                     /translation="MSQTTPDWMSLEAIAALARADVRRALDEDVGSGDLTAALVPAGR
                     RARARVLAREAAVICGAPWVEATLRALDPQVQLRWQVTEGQRCAADQVVLEAEGDARA
                     LLSAERTALNFLQLLSAVASKTASYVQAVAGTRAQIVDTRKTLPGLRLAQKYAVRVGG
                     GVNHRIGLHDAVLIKENHIAAAGGVTAVLRAAQAVAARAAFIEIEVETLAQLREALDA
                     GARMVLLDNMPLPMLREAVALNAGRAVLEISGGVTLDSLRALAETGVDRISVGALTKD
                     VKATDFSMRLQELP"
     gene            16923..18032
                     /gene="nadA"
                     /locus_tag="PKN51_04780"
     CDS             16923..18032
                     /gene="nadA"
                     /locus_tag="PKN51_04780"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNT84446.1"
                     /translation="MSTTIDVEYERPEGAAACSTHVAWARVPAEPSPQERTALKARIR
                     SLLQQKNAVMVSHFYVHPDLQDLAEETGGIVSDSLEMARFGRDHAARTLVVSGVRFMG
                     ETAKILSPEKTVLMPDLGATCSLDLGCPVEEFSAFCDEHPGRTVVVYANTSAAVKARA
                     DWVVTSSCALDIVRALKDAGQKILWGPDRHLGDYIRRETGADMVFWSGACIVHDEFKA
                     LELELLKAQRPQARVLVHPESPAGVIALADAVGSTSAILRAAQEMDATEFIVATDKGM
                     MHKLRQLNPGKTFHEAPTAGNSATCKSCAHCPWMAMNSLAGVVHALETGANAIHVDPA
                     LIPRARRPIDRMLAFTAGLRQGVPAARLVPDLGAV"
     gene            complement(18202..18564)
                     /locus_tag="PKN51_04785"
     CDS             complement(18202..18564)
                     /locus_tag="PKN51_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HNT84447.1"
                     /translation="MPHLTIEYSPNLGAFPEAQAAAELNASVCASPEVLDEADLKTRF
                     VRCAPTFQIGTVLAGRAFVHAQLRLLAGRSPEAKQDLAGRVASVLRRVTPRPDGVMVQ
                     LSVEIVDMDRPSYVKERL"
     gene            complement(18599..20188)
                     /gene="nadB"
                     /locus_tag="PKN51_04790"
     CDS             complement(18599..20188)
                     /gene="nadB"
                     /locus_tag="PKN51_04790"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNT84448.1"
                     /translation="MATLEFDVLIVGSGLAGLSAALHLAPTRRVAVITKRELQEGSSR
                     WAQGGIAAVLANDDSFAAHIQDTQVAGAGLCDLATTRFVVENAPAAIGWLRGLGVPFT
                     LEGEELHLTREGGHSARRIVHATDATGAAVQKTLIDVVRETPGIRVFEHHTLVDLITS
                     RKLGLAGQRCLGLYALDEARDEVVTFRAPQTILATGGAGKVYLYTTNPDTATGDGIAA
                     AWRAGCRVANMEFIQFHPTGLYHPQAKSFLISEAVRGEGGRLLLPPSAGGTRFMPMHD
                     ERAELAPRDVVARAIDFEMKKHGLDCVHLDISHQSPQFLREHFPNILATCASLGIDIT
                     RQPIPVVPTAHFTCGGVLTDLAGRTDLAGLYAVGEVAYTGLHGANRLASNSLLECLVF
                     ARAAAQSIDAATPAPLPALPPWDDSRVTDADESVVISHNWDELRRFMWDYVGIVRTNK
                     RLERAAHRIALLKSEINEFYASFHVTRDLLELRNLVQVADLIVRSAQMRHESRGLHFS
                     RDWPELAAPAAPTILVPPASP"
     gene            complement(20231..20716)
                     /locus_tag="PKN51_04795"
     CDS             complement(20231..20716)
                     /locus_tag="PKN51_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNT84449.1"
                     /translation="MVLTEFDRALIAATQSGLPLVPRPYDAVAAMLGSSSAAVRERLA
                     ALLAAGVVRRIAAVPNHYRLGYTANGMSVWDVDDAEVDRLGELLGSQPGVSHCYRRPR
                     KPGVWRYNLFAMLHGHSREQVLAQAEQVAALLGPACRAHDVLFSSAILKKTGLRLRQN
                     V"
     gene            complement(20858..21259)
                     /locus_tag="PKN51_04800"
                     /pseudo
     CDS             complement(20858..21259)
                     /locus_tag="PKN51_04800"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
     gene            complement(21249..22223)
                     /locus_tag="PKN51_04805"
     CDS             complement(21249..22223)
                     /locus_tag="PKN51_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNT84450.1"
                     /translation="MQARLLNEWQRGFPLSDRPFAQIGQALGATESEVIQACAELSRL
                     GIISRIGGVWAPGVGGSALLCAMAVPPARLEAVAARVSAHPGVNHNYEREHALNLWFV
                     ITGHDATQTEQALAAIESDTGLPVLRLRMVRPYRIDLGFDLHRPARAGTALRQRPPRV
                     AETDIPLAACVEQGLPLLPRPYDLWAARLGSSPAQLHAQLQAWLDAGTLRRFGLVVRH
                     HEVGFAANAMTVFDIEDAQVDALGERLATQPGVTLCYRRERAQGWPYNLYCMVHGRNR
                     EQTLALLNAAIAGAGLGERPRQVLFSRRRFKQTGARYFRPLEAPADGR"
     gene            complement(22318..23511)
                     /locus_tag="PKN51_04810"
     CDS             complement(22318..23511)
                     /locus_tag="PKN51_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNT84451.1"
                     /translation="MTFPTRRAVLGLAFSTSLLAGCAPALLVPPERGGSDLGLVIERE
                     SGSIGVLDTSARTRLGTIDGLGDLSHASVVFARDGARAYIFGRDGALTKVNLRTGRIE
                     GRVMQAGNSIGGAISQDGTLVVAQNYQPGGIKVFDARTLAPLADIPALVDDKASRVVG
                     LADLPGQRFIFSLFDAGQIWIADLSQPTRPVLTKFEQIGKQPYDALVSPNGRHYIAGL
                     FGEDGLAMIDLWAPQPRVQRILPGYGKGEQPLPVFKMPHLRGWSIAGRHAYLPAIGRR
                     EVLVVDTDSWQEVGRIPVAGQPVFVMARPDGRQVWVNFAVPDYDTVQVIDTQTRQVVH
                     SLKPGKAVLHMEFTPRGDTVWISARDDHHVSVIDTASFRTLAVLPAKSPSGIFFASRA
                     ARTGF"
     gene            complement(23508..23852)
                     /locus_tag="PKN51_04815"
     CDS             complement(23508..23852)
                     /locus_tag="PKN51_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNT84452.1"
                     /translation="MSRRPLLLFVLSLAWLPAWAPAAEPGPERQAQLIRMVRQDCGSC
                     HGIRLTGGLGPALSRERMLDIPFDSLLATIYNGRPGTPMPGWKSMISEADATWIAHQL
                     QAGFPLESGPSR"
     gene            complement(23932..25506)
                     /locus_tag="PKN51_04820"
     CDS             complement(23932..25506)
                     /locus_tag="PKN51_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNT84453.1"
                     /translation="MHQSSNPKAPPMTEAEFTRARQIYFERCAGCHGVLRKGATGKAL
                     TPDLTVGKGTEYLKVFIAYGSPAGMPNWLTSGEMDEKTVDLMARYIQHDAPTPPEFSL
                     EDMEKSRKEYVPVAQRPKKKMNNYNINNIMSVTLRDSGEVALIDGDTKQIINIVKTGY
                     AVHISRLSASGRYLYVIGRDARLNLIDLWMEKPDNVAEIKVGLEARSVETSKFKGWED
                     KVAIAGTYWPPQFVLMDGDTLKPRKIVGTRGMDVNNEYHPEPRVAAIVASHQKPEFLV
                     NAKETGKIWMVDYTDLKNLKTTQIDTAKFLHDGGFDSTRRYFLTAANASDKIVVVDTK
                     TSKLEAIVDVGKTPHPGRGANFKHPKFGPVWATSGLGDERIDIIGTDPAKNKQHAWKV
                     VQTLKGQGGGSLFIKTHPKSTNLWVDTALNPDPKISQSVAVFDVRDLSKPPQVIDIAA
                     CANLKDDGAKRVVQPEYNQKGDEVWFSVWSAKNKESAVVVVDDKTRTCKTAIKDPKLI
                     TPTGKFNVWNTQHDVY"
     gene            complement(25862..27079)
                     /locus_tag="PKN51_04825"
     CDS             complement(25862..27079)
                     /locus_tag="PKN51_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNT84454.1"
                     /translation="MHPDAARLWTAPRWALAILLALLGMLGPFSIDTYIPAFAAIGQS
                     LQATPVQMQQTLSAYLLGFAFMNLFHGALSDSFGRRPVILWGLALFALASVGCALSQS
                     VGQLIFFRALQGFSTGAGIVVSRAVIRDMFPPHEAQRVMSQVTIFFGVAPAVAPMIGG
                     LIAQYLNWHAIFWFLVAVGVLLWLANWRLLPETLHASHRQPFQVGHLMRGYGQLLSDP
                     RFMLLALASGVPFNGLFLYVLSAPAFLGEILQLAPTQFFWFFVLNISGIMAGSWLSGR
                     KAGKVSPKNQILQGFRIMLAVSLINLVANALFDPHPLWSMWPMTVFAFGWALMVPVVT
                     LLVLDLHPDRRGMASSLQAVVGSTANGIVAGAVAPLVMHSALGLAVMSAAFMLVGLVA
                     WLWLRRRWPEIGG"
     gene            complement(27160..>27536)
                     /locus_tag="PKN51_04830"
     CDS             complement(27160..>27536)
                     /locus_tag="PKN51_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT84455.1"
                     /translation="EDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDQNNTAPVDA
                     AMQAGLRDVVKDILDSLTPREAKVLRMRFGIEMTSDHTLEEVGKQFDVTRERIRQIEA
                     KALRKLKHPSRSDKLRSFIDSL"
BASE COUNT         4100 a         9179 c         9767 g         4490 t
ORIGIN      
        1 ccgcgcggct agggaaatca ccgacgagag catggctttg acctgcgtca gtcgcacgta
       61 gaatcattcg ccgaccgatc ggtctgttta ttgggctcgg gccgagtcga tgcccgccgt
      121 tcaactttct cctggagacc acccacatgc tcgcaaacaa gaaggcgctg cttgctgccg
      181 ccatcacctt cggctgcacc gccgcctggg ccgacatcaa cgtcggcgtc accttgtcgg
      241 ccaccgggcc ggccgcttcg ctgggcatcc ccgagaagaa caccatcgcg ctgctgccgc
      301 agaccatcgg cgggcagaag atcaactaca tcgtgctcga tgacgcctcc gacaccaccc
      361 agacggtgac caacacgcgc aagctgatca ccgagcgcaa cgtcgacgtc atcatcggct
      421 cgtccaccac gcccacgtca ctggccatga tcgacgtcgt ggccgaagcg cagacgccca
      481 tgatttcgct cggcgcctcg gccgccatca tcgagccgca ggacggcaag aagcgctggg
      541 ccttcaagac gccgcagaac gacatcatga tggcgctggc gatcgccgag cacatggccg
      601 ccaacggcgt caagacggtc ggcttcatcg gcttttccga cgcctatggc gagggctggt
      661 accgcgagac ggtcaaggcg ctggacctga agaagatcaa gctggtggcc aacgagcgct
      721 tctcgcgcac cgacaccgcc gtcaccggcc aggtgctgaa gctgatcggc gccaagccgg
      781 acgccgtgct ggtggccggt tccggcacgc cctcggcgct gccgcagaaa acgctcaagg
      841 agcgcggcta caccggcaag tactatcaga cccacggcgt ggccaacaac gacttcctgc
      901 gtgtgggcgg caaggacgtg gaaggcacgt tgctgccggc cggcccggtg ctggtggccg
      961 accagctgcc ggccgccaac ccggtgcgca agtcggcgct ggaatacatc aacgcctatg
     1021 agggcgccaa cggcaagggc agcgtgtcca ccttcggtgg ccacgcctgg gacgccggcc
     1081 agatcctgaa ggcggccatc ccggtggcgc tcaagggcgg ccagcccggc acgccgcagt
     1141 tccgcaacgc gctgcgcgat gccctggaag gcgtcaagga ggtgcccggc gcgcatggca
     1201 tcttcaacat gtcggcctcc gaccacctgg gtctggacca gcgcgcccgc gtgatggtga
     1261 agatcgaaaa cggccgctgg aagtaccagg aagtcaagta aacccggggc cttcaggcca
     1321 cggcgcggcg accgagggtc gccgcgcacg ttttcaaccg aatgcgccgc ttgcccggcg
     1381 ccgctcgcat ggatcttcag atcgccgtga tgttggtgca ggacggcatc atcaatggcg
     1441 ccatttatgc cctgatggcg ctggcgctgg tgttggtgtt ctcggtcacc cgcgtcatct
     1501 tcattccgca gggtgagttc gtggcctacg ccgcgctggc gatggcggcg ctgcaggccg
     1561 gtcagtcgcc gccgctgctg tggatgctgc tggggctggc ggcgctggtg ctgatcgtcg
     1621 agctggtgcg ccagacgcgc ggcgccgagg tggactggcg cagcacgctg ttctgggccg
     1681 tgctgctgcc gctgctggcg gcgctgatgc tgctggtgct caagccgcag gccatgctgc
     1741 tgcgctcgct ggcggtgctg ctgctggtca ctcccatggg gccgctgctg taccggctgg
     1801 cgtaccggcc gctggccgac gccacggtgc tgatcctgct gatcgtgtcg gtggcgctgc
     1861 acctggcgct ggtcgggctg ggactgttct tcttcggcgc cgagggctcc cgcaccgagg
     1921 ccttctccga cgcccgctgg gacctgctgg gcctgacggt gtcgggtcag tcgctggtga
     1981 tcctgggggt gacggcggcg ctggtggtgg gcatgttctt gttcttcgag cgcaccatcg
     2041 tcggcaaggc gctgcgcgcc accgccatca accgggtcgg cgcgcgcctg atggggatcc
     2101 cgacggcgct gtcgggtgac ctgagcttcg cgctggcggc gctgatcggc gccgtctccg
     2161 gcctgctgat cgcgccggta accaccatct actacgacac cggctttctg atcggcctga
     2221 agggcttcgt ggccgccatc gtcggtgggc tggccagcta cccgctggcg ctggccggcg
     2281 cgctgctggt gggacagctg gaatcgtttg cctcgttctg ggccagcgcg ttcaaggaag
     2341 tgctggtgtt cacgctgatc attccggtgc tctggtggcg ctcgctgcac acgcaccacg
     2401 tggaggacga ggaatgaagc cgatgcacct ggttctggcc ttcatcgtcc tgctgggcgc
     2461 ggcctggggc tttctgccac cgttcaccgt cacgctgctg agctacatcg gcctgtatgc
     2521 gctggtggcc gtgggtctgg tgatgctgac cggcgtgggc ggcatgacct cgtttggcca
     2581 ggcggccttc gtcggcctgg gggcctacgc caccgcctgg atcagcctgt cgcccgaggc
     2641 ggcgcagtgg ctgggggcac tgcccaaggg cgcctacccc tgggtcgggc tgctgctggg
     2701 actggcggtg acggcggcgg tggcctgggt gctgggcgcc gtcacgctgc ggctgtcggg
     2761 ccactacctg ccgctgggca ccatcgcctg ggggctgagc ctgtactacg tgttcggcaa
     2821 cctggagatg ctgggcggct tcaccgggct gtcgggcatc gcgccgctgg aggtgttcgg
     2881 cgtctcgctg gcctcgccgc ggcggctcgg cctggtgatc tgggcggtgc tgctgctggc
     2941 catctggggc ctgcacaacc tgctggattc gcgcgagggc cgggccatcc gggcgctcaa
     3001 gagcgggcgg ctgatggccg agtcgatggg tgtggacacg gcgcgccagc gcatcaaggt
     3061 gttcgtgctg gcggcgctgc tggcggcgct gtcgggctgg ctgtacgtgc tgctccagcg
     3121 cttcgtcaac ccgacgccgt tcgcgctcaa catcggcatc gagtacctgt tcatggcggt
     3181 ggtcggtggc tccggtcacc tgtggggtgc ggtgctgggc gccaccctga tcacgctgtt
     3241 gaaggagcag ctgcaggact ggctgccgcg cctgctgggc agcagcggca atttcgaaat
     3301 cgtggtgttc ggcctgctga tgctgctggt gctgcagcgc gcgcccaacg ggctgtggcc
     3361 gatgctggct ggcgccctgc agcgcttcac cggagcggcg gcacccaggc ggctggccgc
     3421 cggcgcggcc acgctggcgc agcgccagct gccaccggcg ggcgaacaga tcctgcaggc
     3481 cgacaaggtc accaagcgct ttgccggcct gctggccaac aacgcggtcg atctggacgt
     3541 gcgcgccggt gaggtgcatg cgctgattgg ccccaacggc gccggcaaga gcaccttctt
     3601 caacatgatc tccggcgtcg acgatccgac cgaaggccag gtgcgcctga tggggcaggt
     3661 gatgcagggc cagccctcgc gcgccttcgc cgcgctgggc ctgggccgca ccttccagca
     3721 tgtgcgcctg ctgggcgagc gcagcgtgct ggaaaacgtg gctctgggcg cgcaccggcg
     3781 cggccgccag ggctggctgg ccagcatgct gcggctggac cgtgccgaag aggcggcggt
     3841 gctggccgag gccatgcggc agatcgagcg ctgcggcctg gccgaggtgg cccacacccc
     3901 ggccgcggcg ctgccactgg gccagcagcg catcgtcgaa atcgcgcgcg cgctggccgg
     3961 gcagccagcc atcctgctgc tggacgagcc ggcggccggt ctgcggcatc tcgaaaagca
     4021 gggtctggcc acgttgctga cgcagttgcg cagcgagggg ctggccatcc tggtggtgga
     4081 gcacgacatg gaattcgtca tgaacctggc cgaccgcatc acggtgctgg agttcggcac
     4141 cgtgatcgcc cacggcacgc cggccgaggt acaggccaac ccgcgcgtga tcgcggccta
     4201 cctggggggg gacgtgctgt gagtgcgcca ctgctgagcc tgccgcagtt ttcggtcttc
     4261 tacggcccgg tccaggccat ccatggcatc gagttgcatg tgggcgaggg cgagatcgtc
     4321 accgtgatcg gccccaacgg cgccggcaag accacgctgc tgaacgccat catggggttg
     4381 ctgccctcgc agggcgggct gcgcctggcg gggcacagcg tcgcccggcc gacggtcgag
     4441 accatggtgc gccacggtgt tgcactggtg cccgaaaagc gcgagctgtt cggcgagatg
     4501 tcggtcgagg acaacctgct gctgggcggc tttgccctgt ggcggcgtgg ccagcgcgac
     4561 caggcggcgc gcatgaagga gatcttcggc atcttcccgc gcctggagga gcggcgtgcc
     4621 cagatggcgt ccactctgtc cggcggtgag cgccagatgc tggccatcgg tcgcgccctg
     4681 atgagccggc cgcacctgtt gatgctggac gagccctcgc tcggcctggc gccgctgatc
     4741 gtgcgcgagg tgctgcacac ggtgtcggcg ctgcggcgca ccggcgtgtc ggtgctgctg
     4801 gtggagcaga atgcgcgcgc cgcgctcaac gtggccgatc gcggctacgt gctggagatg
     4861 ggcgaggtgg cggtgcaggg cgacgccggc gagctgatgc acgaccagcg cgtgatcgac
     4921 acctacctgg gtgtgggcgg cgcccgcgcg gccgcctgat cgcactcgaa tactatcgaa
     4981 agcatagctg tcggcgctct ccagacaagc gctgaaggcc aatttgactc atcatcagcg
     5041 ggcggggcga ccggccggca ggcctggccg gccaccgccc cgacccttgg aacggagccc
     5101 ttgccatgac cactttgcag agtttcatcg ccggccgctg gatcggccag cagcccgcgc
     5161 aggcgctgag cagcgccatc gacggcgcca ccgtggccca cacgcatgcc gaggcgctgg
     5221 acttcggcga ggcgctgcac cacgcgcgca gcgttggcct ggcggccatg cgccagaccg
     5281 atttcgcgca gcgcgccgcc atgctcaagg cgctggccaa gtacctgggc gagcgcaagg
     5341 aggagctgta tgcgctgtcg ttgcacaccg gcgccacgcg cgccgacggc tgggtcgaca
     5401 tcgaaggcgg caccggcacc ctgttcgcct acgccagcat ggcgcgcgag ctgcccgcct
     5461 gcggcaacct gtggcatgaa ggcccgagca tgccgctggg caagaccggc gcctttgccg
     5521 gcacccacgt gctggtgccg cgcggcggcg tggcggtgca catcaacgcc ttcaacttcc
     5581 cggtctgggg gctgctggag aagttcgcgc cgacctttct ggccggcttg ccctgcatcg
     5641 gcaagcccgc caccgccacc agctttctga ccgaaaagct ggtgcgtctg atccacgagt
     5701 ccggcatcct gcccgccggt gcgctgcagc tggtgatcgg cggcacgggc gatctgctgg
     5761 atcgcctgca ggtgcaggat gtggtcacct tcaccggctc ggccgatacc gcggcgcggc
     5821 tgcgggtcac gcccaacctg gtgcagcgca gcattccctt caacgccgag gccgattcgc
     5881 tcaactgcgc catcctggcg cccgacgtga ccccggacga cgaggagttc gacctgttcg
     5941 tgaaggaagt cgtgcgcgag atgacgcaga aggcgggcca gaagtgcacc gccattcggc
     6001 gcgccctggt gccgtcgcag cacctggacg cggtggccgc acggttgcag gagcgcctgg
     6061 ccaagaccac cgtgggcgac ccgcgcctgg agggcgtgcg catgggcgcc ctggccagcc
     6121 gcgcgcagca gcgcgatgtg gccgagcgcg tggcccagct gcgccagggc gccgaggtgg
     6181 tgtgcggcgg cgacgccgac tttgcgcccc tgggtgaagg cacgcagggt ggggcgttct
     6241 tccagcccac gctgctgctg gcgcgccagc cgcagcagca tgccgcgcac gacgtcgagg
     6301 cgttcggccc agtcagcacg ctgatgcctt acgacggcat cgaacaggcg ctggcgctgg
     6361 ccgcgcaggg tcagggcagc ctggccgcca ccctggtcac gcgcagccac caggtggcgg
     6421 cgcaggtgat accggcgatg gcgcagctgc acggccggct gcagatcctc gatcgcaccg
     6481 ccgcacccga ggccaccggc catggctcgg ccttgcccta cctcaagcac ggcggccccg
     6541 gtcgcgccgg tggtggcgaa gagctgggcg gtctgcgcgc cgtcaggcac ctgctgcagc
     6601 gcgctgcggt gcagggctcg ccggccatgc tggcggcggt cacgggcgat cggccggcct
     6661 gagggcgtcc ggcacagcgt caggacggcc cgggcgggtg cgcggcgcgc ccacacgcca
     6721 gaccttggcc gcccgcccgc gccccggtac cgagcgcggt gcgggcgggt gtgcagcacg
     6781 atctccccac aagtgaaggc gattgaacct tgaaacggca gttgaccgct tgtcaagttc
     6841 gggcgggccg catcgatgcg gggtttcgcc gctgcgatgg ccttgacctc ctgggtgagc
     6901 ccgctaccca cccgcccgca ccgcgctcgg cgcgccatgc ggcgttgctc ctccttgcgg
     6961 gcaccagccc gcggcggcgt tgcgtcttgc ctggcacgcc gatcacggca cgggcggggg
     7021 cgtccaactg ccgtttctag gttgaagccg ccaaaccctg catggatccg gcccccccgg
     7081 atatgaccag caacctacgg ccgttcttgg ggttaaccct tgacagcccg cgctgcctgg
     7141 ccgcgcggga gcgctggcgt tgtgctgcgc ctggccgacg acggggatcc aatcccacga
     7201 tgcctgtccg agatcgattt caggactccg accttgacca gaaagcgtca gcagctatcg
     7261 atatagtagc gttctgcggt tgatgacggt caagaaattc tgggtttcgg cagctcgttc
     7321 ttgacgcaag ttaaggtctg ggtatcacct gggttctacc ctatgcgggt taccacctaa
     7381 atgagcgagg aaacgcacat gagcagcaca gcctcaggcg gctttaccaa ggagatggca
     7441 cgcaacatct tctatggcgg gtcgctgttt ttcatcctga tctttctcgg actggtgttc
     7501 cataccgagc agcgcctgcc cgagcgcacc aaccaggcgg ccatgaccga cgccgtggtg
     7561 cgcggcaagg cggtgtggga gcagaacaac tgcatcggtt gccacacgct gctgggcgag
     7621 ggcgcctact tcgcgcccga gctggccaac gtctaccagc gccgtggtgg cgacgcctcg
     7681 ggcgccgtct tcatcaaggc ctggatgaag gcgcagccca ccggcacccc gggccggcgc
     7741 cagatgccgc agtttcacct gagcgagcag caactcgacg acctggtcga gttcctcaag
     7801 tggactggcg gcatcaatgc cgagcagtgg ccgcccaaca tcgaaggctg atcgccgagg
     7861 agcaacccag atgagcaaca acaccatcct caagtacaag tcgcaggcgg tggccaagcc
     7921 gtacttcatt gcggcgattg cgctgttcgt gggccagatc ctgttcggtc tggtcatggg
     7981 catccagtac atctacggcg acttcctgtt tccgctgatt ccgttcaacg tggcccgcat
     8041 ggtccacacc aacctgctga tcgtgtggct gctgttcggc ttcatgggcg ccgcctacta
     8101 catggtgccc gaagaggccg aaaccgagct gtacagcccc cggctggcgc tggtcatgtt
     8161 ctggatcttc ctggtggccg gcgcgctgac catcgtcggc tacctgctgg tgccctacgc
     8221 ccggctggcc gaggccaccg gcaacaacct gctggccacc atgggacgcg agttcctgga
     8281 gcagccgctg ccgaccaaag tgggcatcgt catcgtggcg ctggccatgc tgttcaacat
     8341 cagcatgacc tacctcaagg gccgcaagac caccatcacc accgtgctgc tgctcggcct
     8401 gtggggcctg gcgctgctgt tcctgttcgc cttcgtcaac ccgcacaacc tggtgcgcga
     8461 caagatgtac tggtggtggg tggtgcacct gtgggtggag ggcgtgtggg agctgatcct
     8521 ggccgccctg ctggcctacg tgctgatcaa gaccaccggc gtggaccgcg aggtggtgga
     8581 caaatgggtg tacgtgatca tcgccttcgc cctgttcacc ggcatcctgg gcacggcgca
     8641 ccactactac ttcatcggtc tgccgggcta ctggcatctg gtgggcaaca tcttctcttc
     8701 gctggagccg atcccgttct tcctgatgac catgttcgcc ttccgcatgg tgcagcggcg
     8761 ccggcgcgac caccccaacc aggcggccgt gctgtgggcc gtgggctgcg cggtgctggg
     8821 ttttctgggc gcgggggtgt ggggcttcct gcacacgctg agcttcgtca actactacac
     8881 ccacggcacg cagatcaccg catcgcacgg tcacctggcg ttctacggcg cctacgtgct
     8941 ggtggtgatt gccctgatca gctacgccat gccgacgctg cgtggacgca tcgccaacag
     9001 ctaccaggcg caggcgctgg aaatgtggtc gttctgggtc atgagcatcg gcatgggcgt
     9061 gatggtgctg gccctgacgg gcgccggcat cgtgcaggtc tggctgcagc gcatgcccga
     9121 aagcggtgcc atgcccttca tggcggcgca ggaccaggtg gtgttcttct actggatgcg
     9181 cctgtggtcc ggcgtggtgt tcctgatcgg cctggtgatg tacgtgtgga gcttcttcgt
     9241 cggtggcgcg ccggccgagg aaaccctgat cgagcgccgc ggcggcctga tgcccagccc
     9301 cgcccagcgc cggctcaggc acgactgagc gcctgacgcg ccctgagacc tcgccgccgg
     9361 cgcggtggcg atgacgatcc gaactccgca ggatttcccg tcatcggcag cgcacggcgg
     9421 cgatgctttt tttctgcatc gtcatccacc cagccccatg gaccgcaccg acacctcccc
     9481 ggccaccacc cccttctatg ccgagcaggc cggcgagtgc cggctgttcg aacacgcctt
     9541 ccggcagcgc ctgccggtgc tgctgaaggg gccgaccggc tgcggcaaga cccgctttgt
     9601 cgagcacatg gcggcgcgcc tgggtcggcc gctcgtcacg gtcagttgcc acgacgacct
     9661 gacggcggcc gacctggtgg gccgctttct gatcggcgac ggcaacacgg tgtggaacga
     9721 tggcccgctg gcgcgtgccg tgcgcaccgg cgccatctgc tacctcgacg aggtggtcga
     9781 ggcgcgcaag gacaccacgg tggtgctgca cccgctggcc gacgaccgcc gcgtgctgcc
     9841 gatcgagcgc accggcgagc tgctgcaggc gacgcccgag ttcatgctgg tggcgtccta
     9901 caacccgggc taccagaacc tgctcaagaa cctcaagccc agcacccgcc agcgcttcgt
     9961 ggcgctggtg ttcgactatc cgcggcccga ggtcgagcag cgcatcatcg aaaccgaagc
    10021 cggtgccgac gccgcgctga gtgcgcgcct gatcaagctg gccacggcgc tgcggcgtct
    10081 gatcgatcag gacctcgagg agaccgtcag cacccgcctg ctggtggtgg ccgcgcggct
    10141 gatcgtctcg ggcctgccgc cgctgaccgc ctgccgcgcc gccatcgtcg acgcgctgac
    10201 cgacgatctg gacaccgctt cggcgctgga ccaagtggtg caggccgtgc tgtcgcatga
    10261 cgcctgaggc aacgaagcca gcgcgccggc gccggggagg gccctgatgg aagagtgggt
    10321 gggtgaacgc tggcaccgct tcatctggcg cgccgcagac cgcagttttg cgcacgcggc
    10381 cgtgcgactg gaggaggtgc agcgcgcggt gcaactgttg tttcgcgccg gcggcggcga
    10441 tccgctggtg cgggtggctc cggccagcgc cgagaaggtg ggcggtccgc gcagctggct
    10501 gcagaagctc gccggccagg gcgagcgcgc cagcatcggc accctggacg agcagacgct
    10561 ggccctgccg ccggtgatcg cggtgtttgc cgacgccgcg ctcaatcgcg acctgtacct
    10621 ctggctggcg gcgcaggcag cctgccatgt ggccacgccg gccggttggc tggccgacaa
    10681 cctggcggcc acgcggcgcg cgctggcctg ctttcccggc ttgcgcacac gctatgcgcg
    10741 gctgctgcag gcgcatctgg cgcaacgccc ggcccccgag cgcctgcagg gcacgccgcg
    10801 cgagcttgaa cgggccatcc aggcggcgct gcgcgcgtcc ctggacggcg tcgatgcagg
    10861 tcgtgacaca accccgccgg cggcgcgcga gccgtgcgcc ccgcaggatc agggagcgtc
    10921 gctcgacggc cgtgcccgcc agccggccag cgtggcgccg gtctggctgt ggctgcaagc
    10981 catgcagaca gcggtcggcc catccgcgac cgcgcccgag gcggacgctg ccgacggcgc
    11041 gccgccccgc ccgcaacagc agccgacccc gcaggacccc aagcgccgtc gcgcgcaacg
    11101 caccgaggac accagcagca agcatggcct ggtgatgttc tttcgcgccg agtcgatcct
    11161 gagctggagc gagttcgtgc gcgtcaaccg caccgcggac gacgaggaca accaggacta
    11221 cagccaggtg gcggacgaca tgcaggaact ggcactggcg ccacgcaagc cgggcgagac
    11281 gctggcggcg cgcatcaagt tcgacctcga cctgcccagc gccgccgccg acgatctgcc
    11341 ggtgggcgag ggcgagccgc tgcccgagtg ggactaccag cgtggcctgc tgctgcccga
    11401 gcattgccgg gcgcagcgtt tgcggcaccg cgcagcctcg ccggatgcta tcgattcgga
    11461 agctgccggc gcagtcccag ccttggaaaa acgcctgaaa accacttcaa ggcaggtgcg
    11521 gcggcgcttc gaggcgctgc gcgctgcgcc gcgctggcaa catggactga cgcagggcga
    11581 ggagctggac ctggacgcct gggtgcgcca ccaggtcgat cggcgcgacg tggcgcaccg
    11641 gcatgtcgag gcgcccgccg tgtacgcccg ccgggtgcgc tcggagcgca gcctgtccag
    11701 cctgatgctg gccgacctgt cgctgtccac cgacgcctac gccaggccgg gcgcgcgcgt
    11761 gatcgacctg gtgcgcgaca gcctgttcgt gttcggcaac gcgctggacg ccgtgggcga
    11821 tccgttcgag atgctgggct tttccagtgt gcggcgcgcc gtgcgcattc atgagctcaa
    11881 gcgcttcggc gagcgctgga acgacgctgc gcgagagcgg gtggccgcca tcaagccggg
    11941 cttttacacc cgcatgggcg cggccattcg ctacggcacg cagcgcctgg ccgcacggcc
    12001 ggagcgccag cgcctgctgc tgatcctgtc cgacggcaag cccaacgacc tggatcacta
    12061 cgaaggccgg cacggcctgg aagacacccg ccacgccgtc catgcggcgc gccaggccgg
    12121 cctggtgccg ttctgcgtca ccatcgacca gcaggccaac gcctacctgc cgcgcctgtt
    12181 cggcacccag gggttcgcgc tggtgcatcg gccggagcaa ctggcgcggg cgctgaccca
    12241 ggtctacgtg cggctgaccg cggccgtgcg ctagactcgt ctgctttgtg agagggcgcg
    12301 gcgcagctgg cgtcggcccc cgttcctgcc gtggatctca agaaattcaa tctgccgcgc
    12361 tacctggcga cgctgccttt gttctcgaac atgcgtgacg aggaactggc gcgcctggcc
    12421 agcggctgcc agttgcgccg gatggagcgc ggcaagctgg tgtttcgcac cggcgagccg
    12481 tgccaggagt tccatgtcac ggtgctgggt caggtcaagc tgttcgccat cgcgccgaac
    12541 ggcaccgaga aggtgatcga gctgtgcggg cccggccaca gctttgccga agccgtcatg
    12601 ttcatggaca tgccctacat gatctcggcg cagaccctga ccgattcgct catcctgacc
    12661 gtcggcaagg cggcggtgct gggtgagatc gccgccaacc ccgacttttc gctgcgcatg
    12721 ctggccgggc tggcgcgccg cctgcacggg ctggtcaagg acgtggaggc ctacaccctg
    12781 cacagcggcg tgcagcgggt catcggctac ctgctgggcg accgggtgag cgagagcgag
    12841 gtggcgtccg aatcgatcac ggtgtcgctg ccggtgagca aggcggccat cgcgtcgcgc
    12901 ctgtcgctga cgccggaata tttctcgcgc gtgctcaacg agctcgaaac cgccggcctg
    12961 atccaggtgg acaagcgcga catccgcatc ccggacaccg cccggctggc caaccaccaa
    13021 ccgctgtgac ggccgtcgac gccctcagtg tgaggtgcct tcggagcgcg tgatcttgtt
    13081 gaacacgttg tacttgccca ccggtttgcg catgggcagg cgcttgactt ccttgaagct
    13141 ggcggcgtcg tagacgatca gggcgccatc gtcctcccac aggctggcca gggcgtacct
    13201 gccgtccttg gtgaattcga tgtgcgccag cgtcttgccc ggcgtgccct cgatgcgggc
    13261 cactggcgcc agcgtgcgct tgtcgatgat ggtcaggatg tgcttggcct tggggctcat
    13321 catcgaatcg gtccaggcgt agggggtctt ttcgtggctg cgcatgaaaa agcccggacc
    13381 gggggtggca atgttcttga ccagctgcca gctcttcatg tcgatcacgc tgatcacgcc
    13441 gtcctgcagg ttggggctgg ccaacacgcg cgtgtcgccg cggtcgaagg tgatgcccga
    13501 gcccaggtgc ggcatgccgg cgatcggcag ggtggcgatc ttgcgccgca cgtccaggtt
    13561 gaccacctgg ccgctgggcg agccgtcggc gtgcgggcgc gaggcgccca gcgcgtaggc
    13621 gtagttctgg ctgaaaaaga agtcgtccag cggttcgtcc agcaccgtgc ggcgcacgcc
    13681 cagaaagccg tccttgggca ggccttcctt catcttgtag tcgtgcacga agccgtcgta
    13741 gatcggctcg gccttggggt cgtagctgat ttcccacagt tcggggatgt ccttgagcgc
    13801 caccacgaag ctgcgccgcg gctcggcgtc gtacacggcc gacacgcgcg agctctggct
    13861 gccgtcgagc gagtgcgccg cataggtgcg ccggtgcttc agctcggcgt cgaacagcac
    13921 caccgtgtgc ggcaggtagt tggcggccat cacccacttg ccgtcgcccg agacggcgat
    13981 gttgcgcatg ttgatgccgg cgcgcacctc ggccaccacc ttcaggttcc acaggtcgta
    14041 cttggtgatc cagccgtcgc gcgagccgaa gtagacgtag cgcccgtccg gtgtgaactt
    14101 gggcccgccg tgcagggcaa agcggctggc aaagcggtgg atgacctcga agcggtcgcc
    14161 gtcgagcagc gagacgtggt ggtcgccgcc ttcgaccacc acgaacaggt tcatcgggtc
    14221 ggcctgccac cgcgggcggg ccggccagtc gcgccagcct gcggtttcct cgcggctggc
    14281 ggcgatgtcg gcgtcgctcc agcgcggcgg cggcgtgacc ggggtgtaga tccactcggc
    14341 cagggcggcg atttcgtcgg ccttcagggc gctggcaaac ggcagcatct gggttgccgc
    14401 gcggccgtcg cggatcacct tgagcgcttc cggcttgcgc agtcgcgcca ggctttcggg
    14461 cagcaacgcg gggcccatgc cgcccaggcg ctgctcgccg tggcagctgg cgcagtgctg
    14521 ctggtagagc gccgccgcgt cgatgctggg cgcggcggcc ggggccggcg cctgggccgc
    14581 cagcgggccg cccagcagcg ccagaacgag caggccagtg cctttgatta cttcaaaaat
    14641 catagctgct tgcgcacgtc agtcgggcgc tggagtgctt tttctttctg atgttcagtg
    14701 gacgacgttg atgacccgct tcttgctctg gaaggcggtg gtttcgatgc gcgtggcgct
    14761 gggctcgacg ccgatctctt cgtccgtcag gtagcagccc gggtcttcgg cccaggcgtc
    14821 gccggtgacc tgctgcgccc gcacgcgggt gttgccgccg cagatcgcca ggtgccggca
    14881 ggcgccgcag cgcccgtgca ccgggcgcgg ccgtgccttc aggccggcca tcagcgggtc
    14941 ggacaggtcg gtccagatgg cggagaacgg gcgcgacttg acgttgccca gcttgtggtg
    15001 ccaccacatg gtgtcggggt gcacgtcgcc caggttgtcg atgttggcca cgttcacgcc
    15061 cgagctgttg ccaccccagg cgatcaggcg ctggcgcacc ggctcggtcc agcgcggcag
    15121 gtgctgctgc acccactgca gcaggtacgc gccgtcggcg tcgttgttgc cggtcacgta
    15181 ttcgtgccgg ccgccggcct gcgcgtcctg ccaggcgcag tcgtacagca gatccagcgc
    15241 ctggcgcgtg gccaggtggt gggcatcctt gccgcggtgg atgttgccgc ggccggcgta
    15301 gttcaggtgg ctgaaataga acttgtccac cccctcgtcg cgcatcaggc gcagcaggtt
    15361 gggcagatcg aaggcgttca gctcggtcat ggtgaagcgc aggcccacct tggtgcccag
    15421 cttctgcagg tggcgcacgg cgcccagcga gcggtcgaag gcgccatcga gccggcggaa
    15481 cttgtcgtgc gtggcgccga tgccgtccag gctgatgccc acgtagtcga agccggtggc
    15541 gtggatgcgt tcggccagcg gctggtcgat cagcgtgccg ttggtcgaca ggccgacgta
    15601 gaacttgagc gccttggcgc gttcggccac ctcgaagatg tcgggccgca gcagcggctc
    15661 gccacccgac aggatgagcg ccggcacctt gaaggccttg agatcgtcca tcacctcgaa
    15721 cacctgcgac gtgctcagct cgccggggta gtcgtggtcg gcgctgaagg cgtagcagtg
    15781 cttgcaggtc aggttgcagc ggcggatcag gttccagatc accaccgggc cgggcgggcc
    15841 gccgcggcgc gccggctcgg ggtaggtgcc ggtggcctcg gcgcgcgcca gctcgtgcat
    15901 gaattgggag atgcggaaca tgaggaaacc ttccagaaca gccagggtcg atgataggca
    15961 gcctgccggc gttggccatg acatgtgtca attccagccg gtgccgcggg ccggcagacg
    16021 ctggcgcggg ccaggcgcta tgcttcgtct cccatgagcc aaaccacccc tgactggatg
    16081 agcctcgagg cgatcgccgc cctggcgcgt gccgacgtgc gtcgcgcact ggacgaagac
    16141 gtgggcagcg gcgacctgac cgccgccctg gtgccggcgg gccgccgcgc ccgcgcccgc
    16201 gtgctggcgc gcgaggccgc cgtgatctgc ggcgcgccct gggtcgaggc gacgctgcgc
    16261 gcgctcgacc cgcaggtgca gctgcgctgg caggtgaccg aaggccagcg ctgcgccgcc
    16321 gatcaggtgg tgctggaggc cgaaggcgat gcgcgcgcgc tgctgtcggc cgagcgcacc
    16381 gcgctcaact tcctgcagct gctgtcggcg gtggccagca agaccgccag ctacgtgcag
    16441 gcggtcgcgg gcacgcgcgc gcagatcgtc gacacccgca aaaccctgcc cggcctgcgc
    16501 ctggcgcaga agtacgccgt gcgcgtgggc ggtggcgtga accaccgcat cggcctgcac
    16561 gacgccgtgc tgatcaagga aaaccacatt gccgccgccg gcggggtgac cgccgtgttg
    16621 cgcgccgcac aggcggtggc ggcgcgggcg gcgtttatcg agatcgaggt cgagacgctg
    16681 gcgcagctgc gcgaggcgct ggacgccggc gcccgcatgg tgctgctgga caacatgccg
    16741 ctgcccatgc tgcgcgaggc ggtggccctg aacgccgggc gcgcggtgct cgaaatctcc
    16801 ggcggcgtca ccctggacag cctgcgtgcc cttgcggaaa ccggggtcga ccgcatctcc
    16861 gtcggagcat tgaccaagga cgtgaaggcc acggactttt ccatgcgtct gcaggagctg
    16921 ccatgagcac caccatcgac gtcgaatacg agcgccccga aggcgcggcc gcgtgttcca
    16981 cccacgtcgc ctgggcgcgc gtgccggccg aaccgtcgcc gcaggagcgc accgcgctga
    17041 aggcgcgcat ccgcagcctg ctgcagcaaa agaacgccgt catggtgtcg cacttctacg
    17101 tgcaccccga cctgcaggac ctggccgaag agaccggcgg catcgtcagc gactcgctgg
    17161 agatggcgcg cttcgggcgc gatcacgcgg cgcgcacgct ggtggtcagc ggcgtgcgct
    17221 tcatgggcga gacggccaag atcctgagcc ccgaaaaaac cgtgctcatg cccgacctgg
    17281 gcgccacctg ctcgctcgac ctgggctgcc cggtggagga gttcagcgcc ttctgtgacg
    17341 agcaccccgg gcgcacggtg gtggtttacg ccaacaccag cgcggccgtg aaggcgcgcg
    17401 ccgactgggt ggtgacctcg agctgcgcgc tcgacatcgt gcgcgcgctc aaggacgcgg
    17461 gccagaagat cctgtggggg ccggaccgcc acctgggcga ctacatccgg cgcgagaccg
    17521 gcgccgacat ggtgttctgg agcggcgcct gcatcgtgca cgacgaattc aaggcgctgg
    17581 agctggagct gctgaaggcg cagcgcccgc aggccagggt gctggtgcac cctgaaagcc
    17641 cggccggcgt gatcgccctg gccgacgccg tgggttccac cagcgccatc ctgcgcgcgg
    17701 cgcaggagat ggacgccacc gaattcatcg tcgccaccga caagggcatg atgcacaagc
    17761 tgcgccagct caaccccggc aagacgtttc acgaggcccc caccgccggc aacagcgcca
    17821 cctgcaagag ctgcgcccac tgcccctgga tggccatgaa cagcctggcc ggcgtggtgc
    17881 atgcgctgga aaccggcgcc aacgccatcc atgtcgatcc ggccctgatc ccgcgcgccc
    17941 gccggcccat cgaccgcatg ctggcgttca ccgcaggcct gcgccagggc gtcccggcgg
    18001 cacgcctggt gcccgatctg ggtgccgtct gatgctatcg taaacatagc tgctggcgct
    18061 ttctgggtct gcgctgcagc cgtttttaac catcctcatc gaggccggcc gaaccccctg
    18121 cgccggcatg accgatgcct tctttgtcat cgacgcgcag cgaggttgtc ggtcatctgt
    18181 catcctgagg gcgagatccc gtcagagccg ctccttgacg taggacggcc ggtccatgtc
    18241 gacgatctcc acgctcagct gcaccatcac gccgtccggg cgcggggtga cgcggcgcag
    18301 cacgctggcc acgcggcctg ccaggtcctg cttggcctcg gggctgcggc cggccagcag
    18361 gcgcagctgg gcatgcacga aggcgcggcc ggccagcacg gtgccgatct gaaacgtggg
    18421 tgcgcagcgc acgaagcggg tcttgaggtc ggcctcgtcc agcacttcgg gactggcgca
    18481 gacgcttgcg ttgagctcgg cggcggcctg ggcttcgggg aaggcgccga ggttggggct
    18541 gtattcgatg gtcaggtggg gcatggggca gcgctccggt ggttgacgag cgtccagctc
    18601 agggtgaagc cggcggcacc aggatggtgg gcgccgccgg tgcggccagc tcgggccagt
    18661 cgcggctgaa gtgcaggccg cggctttcgt ggcgcatctg ggcgctgcgc acgatcaggt
    18721 cggccacctg caccagattg cgcagctcca gcaggtcgcg cgtgacgtgg aagctggcgt
    18781 agaactcgtt gatctcgctt ttcagcagcg caatgcggtg cgcggcgcgc tccaggcgct
    18841 tgttggtgcg cacgatgccc acgtagtccc acatgaagcg gcgcagctcg tcccagttgt
    18901 gcgagatgac gaccgattcg tcggcgtccg tcacgcggct gtcgtcccag ggcggcaggg
    18961 caggcagggg cgccggcgtg gcggcgtcga tcgactgcgc ggccgcgcgc gcaaacacca
    19021 ggcattccag cagcgagttg ctggccagcc ggttggcgcc gtgcaggccg gtgtaggcca
    19081 cctcgccgac ggcgtacagg ccagccaggt cggtgcggcc ggccaggtcg gtgagcacgc
    19141 cgccgcaggt gaagtgcgcc gtgggcacca ccgggatggg ctgccgggtg atgtcgatgc
    19201 ccaggctggc gcaggtggcc aggatgttgg ggaagtgttc gcgcaggaac tgcgggctct
    19261 ggtgcgagat gtccaggtgc acgcagtcca ggccgtgctt cttcatttcg aaatcgatgg
    19321 cgcgcgccac cacgtcgcgc ggcgccagct cggcgcgctc gtcgtgcatg ggcatgaagc
    19381 gcgtgccccc ggccgagggg ggcagcagca ggcgcccgcc ttcgccgcgc accgcctcgc
    19441 tgatcagaaa ggacttggct tgcgggtggt acaggccggt gggatggaac tggatgaatt
    19501 ccatgttcgc cacccggcag ccggcgcgcc aggcggcggc gatgccgtcg ccggtggcgg
    19561 tgtcggggtt ggtggtgtag aggtagacct tgcccgcgcc gccggtggcc aggatggttt
    19621 gcggggcgcg gaaggtcacc acctcgtcgc gcgcctcgtc gagcgcgtac aggcccaggc
    19681 agcgctggcc ggccaggccc agcttgcggc tggtgatcag gtccaccagg gtgtggtgct
    19741 cgaacacgcg gatgccgggc gtttcgcgca ccacgtcgat cagcgttttc tgcaccgcgg
    19801 cgccggtggc gtcggtggcg tgcacgatgc gccgcgcgct gtggccgcct tcgcgcgtca
    19861 ggtgcagctc ctcgccttcg agcgtgaacg gcacgcccag gccgcgcagc cagccgatgg
    19921 cggcgggggc gttctcgacc acgaagcggg tggtggccag atcgcacagg ccggcgccgg
    19981 ccacctgggt gtcctgaatg tgggcggcaa aactgtcgtc gttggccagc acggcggcga
    20041 tgccgccttg cgcccagcgg ctggagcctt cctgcagctc gcgcttggtg atcaccgcca
    20101 cacgccgcgt gggcgccagg tgcaaggcgg ccgacaggcc ggccaggccg ctgcccacga
    20161 tcagtacgtc gaattccagt gtggccatgg cgcatggtaa tcggtcgact gcgccggccg
    20221 cagccgggct tcagacgttc tggcgcagcc gcagaccggt tttcttgagg atggcgctgg
    20281 aaaacagcac gtcgtgcgcg cggcaggcag gcccgagcag ggcggccacc tgctcggcct
    20341 gggccagcac ctgttcgcgc gagtggccgt gcagcatggc aaacaggttg tagcgccaca
    20401 cgccgggctt gcgcgggcgg cggtagcagt gactgacgcc cggctggctg cccagcagct
    20461 cgcccaggcg atcgacctcg gcgtcgtcga cgtcccacac gctcatgcca ttggcggtgt
    20521 agcccaggcg atagtggttg ggcacggcgg cgatgcggcg caccacgccg gcggccagca
    20581 gcgccgccag gcgctcgcgc accgccgcgc tgctgctgcc cagcatggcc gccacggcgt
    20641 cgtagggacg tggcaccagc ggcaggccgg actgggtggc ggcgatcagc gcgcgatcga
    20701 attcggtcag caccagcgcg gtcgcagccg gcgccgcctg cgcggcgggc ggcgccgggt
    20761 cagggcggtc cagcagcggc aggtacaggc cgacgaagta ttcgcgctcc ttcgggaagg
    20821 catgcaccgg caggccggtg gcggcttcga tctggtcgca ggtggcccag gcgacggccg
    20881 gggtctcggc ggcaaccacg aaccacatgt tgagcgcgtg ctcgcgacga tagttgtggg
    20941 ccacctcggg cagggcgttg acctgggccg tgacttcggc gtagcgctgc tgcggcacct
    21001 gcagcgccgc caggatgaac tggccgccgg cgcgctcgat ctggtagagc gggcccaggc
    21061 gcgtcagcac gcccgaaccg agcagctggg tcaggcgatc gagcaccgcc gcctcgctca
    21121 ggcccagttc ggcgcccaga tcggcgaacg ggcgctcggt cagtggcacg ctgcgttgca
    21181 ggcggtcgat caggcgggcg tcctgcaggt cgatgtcggc cggcagacgt ggcgcgtcgc
    21241 ggtgcgtgtc agcggccatc ggcgggtgct tccagcgggc ggaaatagcg ggccccggtc
    21301 tgcttgaagc ggcggcggct gaacagcacc tggcgcggcc gttcgcccag gccggcgccg
    21361 gcgatggcgg cgttcagcag cgccagggtc tgctcgcggt tgcggccgtg caccatgcag
    21421 tacaggttgt agggccagcc ctgggcgcgc tcgcgccggt agcacagggt gacgccaggc
    21481 tgcgtggcca ggcgctcgcc cagggcatcg acctgcgcgt cctcgatatc gaacaccgtc
    21541 atggcgttgg cggcaaagcc gacctcgtgg tggcgtacca ccaggccgaa gcggcgcagc
    21601 gtgccggcgt ccagccacgc ctgcagctgc gcatgcagtt gcgcggggct gctgcccagg
    21661 cgtgcggccc agaggtcgta gggccgcgga agcagcggca ggccctgctc gacgcaggcg
    21721 gccaggggga tgtcggtctc ggcgacgcgg ggcgggcgct gccgcagtgc ggtgccggcg
    21781 cgcgccgggc ggtgcaggtc gaagccgagg tcgatgcggt agggccgcac catgcgcagg
    21841 cgcagcaccg gcagcccggt gtcggattcg atggctgcca gcgcctgttc ggtctgcgtc
    21901 gcgtcgtggc cggtgatcac gaaccagagg ttgagcgcat gctcgcgttc gtagttgtgg
    21961 ttcacccccg ggtgggccga aacccgggcg gcgaccgctt cgagccgagc gggcggcacc
    22021 gccatggcgc acagcagcgc gctgccgccc acccccggtg cccagacgcc accgatgcgg
    22081 ctgatgatgc ccagccgtga cagctcggcg cacgcctgga taacttcaga ttccgtagcg
    22141 cccagcgctt gcccgatctg cgcaaacggc ctgtcagaca ggggaaaccc acgttgccat
    22201 tcgttcagca ggcgagcctg catgccgggg tcgttgtccc acgacgacgt ggaccactcg
    22261 gacgctgcgg ccgggtccgc gcgctgcgac agctggggca gaacccgggg cacggcgtca
    22321 gaacccggtg cgcgcggcgc gcgaggcaaa gaagatgccg ctgggtgact tggccggcag
    22381 cacggccagc gttctgaagc tggcggtgtc gatgaccgac acatggtggt cgtcgcgtgc
    22441 gctgatccac accgtgtcgc cgcgcggtgt gaattccatg tgcagcaccg ccttgccggg
    22501 cttgaggctg tgcaccacct ggcgcgtctg ggtgtcgatg acctgcaccg tgtcgtagtc
    22561 gggcacggcg aagttgaccc acacctggcg gccgtcgggc cgcgccatca cgaacaccgg
    22621 ctggccggcc accgggatgc ggccgacctc ctgccagctg tcggtgtcca ccaccagcac
    22681 ctcgcgccgc ccgatggcgg gcaggtaggc gtggcgcccg gcgatcgacc agccgcgcag
    22741 gtgcggcatc ttgaacaccg gcagcggctg ttcacccttg ccgtagccgg gcaggatgcg
    22801 ctgcacgcgc ggctgcggcg cccacagatc gatcatggcc aggccgtcct cgccgaacag
    22861 gccggcgatg tagtggcggc cattcgggga aaccagcgcg tcgtagggct gcttgccgat
    22921 ctgctcgaac ttggtcaaca ccgggcgcgt gggctgcgac aggtcggcga tccagatctg
    22981 acccgcatcg aacagcgaga aaatgaagcg ctggcccggc agatcggcca ggccaaccac
    23041 gcgcgaggcc ttgtcgtcga ccagtgccgg aatgtcggcc agcggcgcca gcgtgcgtgc
    23101 gtcgaacacc ttgatgccgc ccggctgata gttctgtgcc accaccagcg tgccgtcctg
    23161 agagatggcg ccgccgatcg agttgccggc ctgcatcacg cggccttcga tgcggccggt
    23221 gcgcaggttg accttggtga gcgcgccatc gcgcccgaag atgtaggcgc gcgcgccgtc
    23281 gcgggcaaac accaccgagg cgtgcgacag gtcgcccagc ccatcgatgg tgcccaggcg
    23341 ggtgcgcgcc gaggtgtcga gcacgccgat ggagccgctt tcacgttcga tcaccagccc
    23401 cagatcgctg ccgccgcgct ccggcggcac cagcagggcg ggcgcgcagc cggccagcag
    23461 ggaagtggag aaggcgaggc cgagcaccgc tcggcgtgtg ggaaacgtca tcgtgaaggg
    23521 cccgattcaa gaggaaaacc ggcctgcagc tggtgggcga tccaggtggc gtcggcctcc
    23581 gagatcatgc ttttccagcc tggcatgggg gtgccggggc ggccgttgta gatggtggcg
    23641 agcaggctgt cgaagggtat gtcgagcatg cgttcgcggg agagcgccgg ccccagcccg
    23701 ccggtcaggc ggatgccatg acacgagccg caatcctggc gcaccatgcg gatcagctgc
    23761 gcctggcgtt caggtccggg ctcggcggcc ggcgcccagg cgggcagcca ggccagggac
    23821 aagacgaaga gcaacagggg gcgtcgggac atcgggcgaa gatagcaaaa acggggcgcc
    23881 acaaacacca agggccgccg gggcggcggc ccttggtgcg gggctaggcc atcagtacac
    23941 gtcgtgctgg gtgttccaga cgttgaactt gccggtcggc gtgatcagct tgggatcctt
    24001 gatcgccgtc ttgcaggtgc gcgtcttgtc gtccaccacc accacggcgg attccttgtt
    24061 cttggcgctc cacacggaga accagacttc gtcacctttc tggttgtatt ccggctgcac
    24121 cacgcgcttg gcgccgtcgt ccttcaggtt ggcgcaggcg gcgatgtcga tcacctgcgg
    24181 cggcttgctc aggtcgcgga cgtcgaacac ggccacgctc tggctgatct tgggatcggg
    24241 gttgagcgcc gtatcgaccc acaggttggt cgacttcggg tgcgtcttga tgaacagcga
    24301 accaccaccc tggcccttga gcgtctgcac caccttccag gcgtgctgct tgttcttggc
    24361 cgggtcggta ccgatgatgt cgatgcgctc gtcacccaga cccgaggtgg cccacaccgg
    24421 accgaacttc ggatgtttga agttggcgcc gcggccgggg tgcggggtct tgcccacgtc
    24481 cacgatggct tccagcttgc tggtcttggt gtcgaccacc acgatcttgt cggacgcatt
    24541 ggcggccgtc aggaagtagc ggcgggtcga gtcgaagcca ccgtcgtgca ggaacttggc
    24601 ggtgtcgatc tgcgtcgtct tcaggttctt caggtcggtg tagtcgacca tccagatctt
    24661 gccggtttcc ttggcgttga ccaggaattc gggtttctgg tggctggcca cgatggcggc
    24721 cacgcgcggc tcggggtggt attcgttgtt gacgtccatg ccacgcgtgc cgacgatctt
    24781 gcgcggcttg agcgtgtcac cgtccatcag cacgaactgc ggcggccagt aggtaccggc
    24841 gatcgccacc ttgtcttccc agcccttgaa tttgctggtc tcgaccgagc gcgcttccag
    24901 tccaaccttg atttcagcca cgttgtcggg cttttccatc cacaggtcga tcaggttcag
    24961 acgggcgtcg cggccgatca cgtacaggta gcgccccgaa gccgacaggc gcgagatgtg
    25021 caccgcgtag ccggtcttga cgatgttgat gatctgcttg gtgtcgccgt cgatcaacgc
    25081 cacctcgccg gaatcacgca aggtgaccga catgatgttg ttgatgttgt agttgttcat
    25141 cttcttcttg ggacgctgcg ccaccggcac gtattccttg cgcgacttct ccatgtcctc
    25201 cagcgagaac tcgggcggcg tcggcgcgtc gtgctggatg tagcgggcca tcaggtcgac
    25261 cgtcttttcg tccatttcgc cgctggtcag ccagttcggc atgccggccg gcgaaccgta
    25321 ggcaatgaag actttcaggt actcggtgcc tttgccgacg gtcaggtcgg gcgtcagcgc
    25381 cttgccggtg gcgcccttgc gcagcacgcc gtggcaacca gcgcagcgct cgaagtagat
    25441 ctggcgggcg cgggtgaatt cggcctccgt catgggcggc gccttggggt tgctggactg
    25501 gtgcatggtc gaagggtcga ccgcgtaggg cgcgccctcg tacttggctt cagcgctgga
    25561 gggcgaggtg gtggggccgc ccggcttcgc gggttcgccg cccgcctggg cgtatgcacc
    25621 gctgacaccg agcagcagcg ccgtgctcag tacggcagtc agcggcgaaa gtttggactt
    25681 cctcttgaag gtgttcatca tgttgttccg tgtcaccgca gcagccggat tcgagggccg
    25741 ggtgatggct gctggatatc actcccggtc agcgcaatct tgcaggcaca gcccggtttg
    25801 aaccttgact tggttcaaat tgcgtgcatt gcccgccgct ggggcaccgg gcgggtctcg
    25861 ctcagccgcc gatctcgggc cagcgccggc gcagccacag ccaggccacc aggccgacca
    25921 gcatgaaggc ggccgacatc acggccagcc cgagcgccga atgcatgacc agcggggcca
    25981 ccgcgccggc cacgatgccg ttggcggtcg agcccaccac cgcctgcagc gacgacgcca
    26041 tgccacgccg atcggggtgc aggtcgagca ccagcagggt cactaccggc accatcagcg
    26101 cccagccgaa ggcgaacacc gtcatcggcc acatcgacca cagcgggtga gggtcgaaca
    26161 gggcgttggc cacgaggttg atcagcgaca ccgccagcat gatccgaaaa ccctgcagga
    26221 tctggttctt ggggctgacc ttgccggcct tgcgccccga cagccaggag ccggccatga
    26281 tgccgctgat gttcagcacg aagaaccaga agaactgcgt cggcgccagt tgcaggatct
    26341 cgccgaggaa ggccggcgcc gacagcacgt agaggaacag gccattgaag ggcacgcccg
    26401 aggccagcgc cagcagcatg aagcggggat cgctgagcag ctggccgtag ccgcgcatca
    26461 ggtggccgac ctggaacggc tggcggtggc tggcgtgcag ggtttcgggc agcaggcgcc
    26521 agttggccag ccacagcagc acgcccacgg cgaccaggaa ccagaagatg gcgtgccagt
    26581 tcaggtactg ggcgatcagg ccgccgatca tcggcgccac cgccggggcg acgccgaaga
    26641 agatggtgac ctggctcatc acgcgctgtg cctcgtgggg cggaaacatg tcgcggatga
    26701 ccgcgcgcga gaccacgatg ccggcgccgg tggaaaagcc ctgcagcgcg cgaaagaaga
    26761 tcagctgtcc gacgctctgc gacagcgcgc agccgaccga ggccagggcg aacagcgcca
    26821 ggccccacag aatgaccggc cggcgcccga agctgtcgga cagcgcgcca tgaaacaggt
    26881 tcatgaaggc gaagccgagc aggtaggcgc tcagggtctg ctgcatctgc accggcgtcg
    26941 cctgcagcga ctggccgatg gcggcaaacg ccggaatgta ggtgtcgatc gagaacggtc
    27001 ccagcatgcc cagcagcgcc agcagaatgg ccagggccca gcgcggggct gtccagagtc
    27061 gggcggcgtc ggggtgcatg ggaaccagta tgctataaaa aaagaagctg gccgcgcagt
    27121 ctgggcgccg gccagcgtag gttttatcgt tgaatcggct tacagcgaat cgatgaagct
    27181 gcgcagcttg tcgctgcggc tggggtgctt gagcttgcgc agggccttgg cctcgatctg
    27241 acggatgcgc tcgcgcgtga cgtcgaactg cttgcccact tcttccagcg tgtggtcgct
    27301 ggtcatctca atgccgaagc gcatgcgcag caccttggcc tcgcgcgggg tcaggctgtc
    27361 gaggatgtcc ttgaccacgt cgcgcaggcc ggcttgcatg gcggcgtcga caggcgcggt
    27421 gttgttctgg tcctcgatga agtcgcccag gtggctgtcg tcgtcgtcgc cgatgggtgt
    27481 ttccatggag atcggctcct tggcgatctt catgatcttg cggatcttgt cctcgg
//
LOCUS       DAOQTZ010000026        27312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2067_length_27312_cov_14.424441, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000026 DAOQTZ010000000
VERSION     DAOQTZ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 27312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27312
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2067_length_27312_cov_14.424441"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1075
                     /locus_tag="PKN51_04835"
     CDS             323..1075
                     /locus_tag="PKN51_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84456.1"
                     /translation="MKRTLRISAFVLAAGLATLSGCATKAPIPLAENFDLTVQPKVRS
                     AGHWELLSNDVVTQTLASLKNAQVPANTPMVVGLPANPSAFDRGFREFLITKLVQSGQ
                     TVYMQPDQAAAVAWQTQACAATPACRDQDALEVTYQTQVVRHDSPRPHFVPGQFTVLA
                     AGLMAAYGLRLEHLDAKLIATLGLAAAADYGASVYTGGPTNTELILTTTVSNRGRFVS
                     RKTDVYYLENVDAPLFMRSAPGYKAVNLKVVS"
     gene            1072..1644
                     /locus_tag="PKN51_04840"
     CDS             1072..1644
                     /locus_tag="PKN51_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNT84457.1"
                     /translation="MRKFAPLALTLALASLAGCSTFGDAAPVRRAGPTYETAAHNAFL
                     DSGRAAVGKLVEGLPADTLAGGPVLVATVVNVNNLTRSAPLGRTLSEQYASHMAAQGF
                     NVKEVKLRGELFVREGTGELLLSRELREIARSQNAAVVLVGTYSPASDYTYVSIKLVR
                     TEDSRILRGHDYALPNDRDVQRLLQEPSFR"
     gene            1890..2810
                     /locus_tag="PKN51_04845"
     CDS             1890..2810
                     /locus_tag="PKN51_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNT84458.1"
                     /translation="MLNNKKSCALLLAAGAALLLSGCETTSMKMGSPEAKTVATGSAA
                     GAATSGENTALEKCDSPLGTVSLVENQTAGWYTILTGEYKLPPTANLLRLLVQQSNCF
                     VVVERGAAGMRAMSRERELMQSGEMRGGSNFGKGQMVASDYALSPEIVFSADNAGGIG
                     GVLGGLIGGGSGRAVAAIGGGLQTREASAMLTLVDNRSGVQVAASEGSASKTDLNLFG
                     GLFGGRAGAGLGGYTNTPQGKVISAAFMDAFNQMVRSLRNYRAQSVQGQGLGGGGRLG
                     VDGGAAPSQTYVPENAPKSAPSKRAPTRRR"
     gene            3097..3723
                     /locus_tag="PKN51_04850"
     CDS             3097..3723
                     /locus_tag="PKN51_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNT84459.1"
                     /translation="MSLLELIRRRRASLPDWVQEWLDVLVPALQVALIVLAAWLLHWL
                     LKRLVLRLARLYALPPAIVLPLRGVLRWLIIVATALLVLGRLGVSAEVLWTAFTGFAA
                     VAAVAFFAAWSVLSNLFCALLIMVAQPFRLGDDLEVLDSAEKPGAKGRVVDINLLYTT
                     LQETDGGGRLLQIPNALFFQRVVRRWRGGMAAAAADPSAAAPAGHEPG"
     gene            complement(3759..5186)
                     /locus_tag="PKN51_04855"
     CDS             complement(3759..5186)
                     /locus_tag="PKN51_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT84460.1"
                     /translation="MNAPAAVHPVQPRIQPRPVPGVLIDALRSRFGAQFSDAQAVREQ
                     HGRDESAFTTVPPPAAVVFAESTQDVSDLVRLCAQHQVPVIPFGVGSSLEGHLLAVQG
                     GISVDLSRMNRVLSIQAEDLLVTVQPGVTREQLNAEIRDTGLFFPIDPGANATLGGMS
                     ATRASGTNAVRYGTMRENVLALEVVTASGEVIRTGTRARKSSAGYDLTRLFVGSEGTL
                     GVITEITLRLYPLPEAVSAAICSFPSIAEAVGTVIQIIQLGVPIARVELLDARTVRAV
                     NQHSKLSLREAPMLLMEFHGSPAGVREQAETVQAIAGEHGGAGFEWADTPEERTRLWT
                     ARHKAYFAGVQSRPGCRCITTDVCVPISRLADAVLASVQEADASGIPYFLVGHVGDGN
                     FHMGYLIDPDDDAERARAEQLNHQVVERALALGGTCTGEHGVGLHKMGFLLDEAGAGA
                     VALMRTIKQALDPHNILNPGKVLAP"
     gene            complement(5302..5730)
                     /locus_tag="PKN51_04860"
     CDS             complement(5302..5730)
                     /locus_tag="PKN51_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNT84461.1"
                     /translation="MPIWKQAVSPEILNTISRDTAVSHLGIEFIEVGDDFLRARVPVG
                     AHTRQPYGLLHGGVSVVLAETLGSCGAVYACPEGWRAVGLDINANHLRAATRGWVTGT
                     ARPVHIGRTTQVWQIDMHNDAGELTCVSRLTMAVLAPRQS"
     gene            5799..6638
                     /gene="aroE"
                     /locus_tag="PKN51_04865"
     CDS             5799..6638
                     /gene="aroE"
                     /locus_tag="PKN51_04865"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNT84462.1"
                     /translation="MPDRYAVIGHPIAHSKSPLIHGLFAQATGQDIVYEAIDGGAAPG
                     GFQRALADFRAAGGRGLNVTLPFKLAALAVADLASEDARLAGAANALRFEDGRIEARN
                     FDGIGLVRDIEVNLGLPLAGRRVLLLGAGGATRGAVRPLARAGAARIVIANRTADKAR
                     TLAAELAPHLDGVPLVGGGLAALATGEAFEVVINATSASLSGAAPELPACAFAPGALA
                     YDMVYGQGLTPFLRLARAAGCARLADGVGMLVEQAAEAFAWWRGVRPDTREALARLTV
                     PLA"
     gene            6653..7102
                     /gene="aroQ"
                     /locus_tag="PKN51_04870"
     CDS             6653..7102
                     /gene="aroQ"
                     /locus_tag="PKN51_04870"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNT84463.1"
                     /translation="MNLLVLHGINLNMFGQRDPAHYGSATLADVDAALQRLAGELSAR
                     VECFQTNHEGALCERIHQAHRDAVDGVLINAGAWTHTSIAIRDALAILQCPIVEVHMS
                     NIHAREPFRHHSFIAGLARGQICGFGVDSYLLGLRALVGQAQAAIKK"
     gene            7113..7694
                     /locus_tag="PKN51_04875"
     CDS             7113..7694
                     /locus_tag="PKN51_04875"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNT84464.1"
                     /translation="MGKRLTQIATRTGDDGTTGLGDNTRVSKASGRPQAMGDVDELNS
                     HIGLLLCEALPPAVRELLIDIQHQLFNLGGELAMPGFELLKDEALAQLDAALAQHNAA
                     LPRLEEFILPAGTRAACQAHVCRTVARRAERAVVALGQQEPLRAAPRQYLNRLSDLLF
                     VLSRVLNRYRPDGSVGDEVYWKSERLARASTEG"
     gene            complement(7706..8497)
                     /locus_tag="PKN51_04880"
     CDS             complement(7706..8497)
                     /locus_tag="PKN51_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT84465.1"
                     /translation="MTEMPTRRQVLTLSALLAPTLNGPAWAQDDKACALVFVHGKWGN
                     TQYVGFFTRRLEPYCKVRVAEAPWSLRRGYDAPYSRALDDMAAEVKALRDQGYRRVLV
                     GGHSFGANAAMAYMARVGDADGVVAVAPGHSPAMMYARGIGKDAVDKARQLVQAGQGS
                     ETLTMEDLNQGQRKSVRMSAEVLWSYFDPEGLGHMPASAAAFKKPVPLLWVVGTADPI
                     YRGGEEFAFRRAPPHPASQYLVYSGGHGDFEQTAEPVLAWLKALP"
     gene            complement(8541..9827)
                     /locus_tag="PKN51_04885"
     CDS             complement(8541..9827)
                     /locus_tag="PKN51_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNT84466.1"
                     /translation="MSLFNWQEKPASQLAAGGVVHIDERLPWPQTVAMGVQHVIAMFG
                     ATVLAPILMGFDPNLAIFMSGVGTLLFYFITGGRVPSYLGSSFAFIGVVIAATGYAGQ
                     GPNPNIAVALGAIIVCGLLYALIGALVNAVGTRWIERLMPPVVTGAVVAVIGLNLAAI
                     PVKNMAASNFDSWMQLVTFVSVALVAVFARGMVQRLLILVGLAIASVIYVLLANGLGL
                     GKPMDFSGIASAAWVGLPRFTAPVFEGNAMLLIAPVALILVAENLGHLKAVAAMTGRN
                     LDPYIGRAFTADGVATMISGAAGGTGVTTYAENIGVMAATRIYSTAVFVVAALMALAL
                     GFSPKFGALIQAIPLPVMGGVSIVVFGLITIAGARIWVENKVDFGDPANLLTAAITLI
                     LGTGDFTLKFGSFALGGIGTATFGAVLLHALLRRRA"
     gene            complement(9984..10745)
                     /locus_tag="PKN51_04890"
     CDS             complement(9984..10745)
                     /locus_tag="PKN51_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT84467.1"
                     /translation="MTRPAYTTEPLFQQIAQTLRQRLRDGSYPVGIPLPGERELTREF
                     GVARSTVRAALARLQEEGCITRLPGQGTLPVAEPSTGEHSKIRNGLLENILRFGQRTR
                     TTLVSTQYMAAPDAVAQALQLPAGSRVMRVVRVRKSHRTPLLCTEAYLPAAVAEGIAP
                     QMLHDMPLLEAIEQAGHAFAQGEQELMAVVAPAEVAELLRIAPGAALLRVNRVVADAQ
                     GRPLQYLIGHYAPDHYHYRMRLSRTGGSTTVWITH"
     gene            complement(10803..11657)
                     /locus_tag="PKN51_04895"
     CDS             complement(10803..11657)
                     /locus_tag="PKN51_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNT84468.1"
                     /translation="MSLKQRLAAPGITIAPGVYDALTALLVQQAGFPAAYLSGASIAY
                     TRLGRSDIGLLGLDDVAQVTSCIRERVELPLIVDADTGFGNALNVMRSVRQLERAGAS
                     AIQLEDQTSPKRCGHLQGKSVIATREMVGKLRAAVDARRDDDTLIIARTDAVAVEGFD
                     AALERAARYAEAGADLLFVEAVRSRDQMQQLNQVLGALCPLMANMVEGGMTPVTSAQE
                     LAEIGYRLVIFPGGTVRALTHALQGYLASLHTHGSTLPWRDRMLDFDALNAVIGTPEL
                     LALGQRYD"
     gene            complement(11654..12220)
                     /locus_tag="PKN51_04900"
     CDS             complement(11654..12220)
                     /locus_tag="PKN51_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNT84469.1"
                     /translation="MNHSTSPAPVNAQPRPGRCWVLGDDINTDYLAPGKYMKFGIEDI
                     ARHCLEDVLPHFAAEVRAGDLIIAGRNFGTGSSREQAVEVLRHLGVQCVIARSFAGLF
                     YRNGFNLGLPLLLGPADMPAPAWGIAPGTPAVLDLAQARLQLPDARQLSCQPIPTHLL
                     ALVADGGLVPHLRRRIERQRPQHTRTAP"
     gene            complement(12217..13491)
                     /locus_tag="PKN51_04905"
     CDS             complement(12217..13491)
                     /locus_tag="PKN51_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNT84470.1"
                     /translation="MPATLAQKLVARACGQTQVQPGQIVTCRVDLAMMHDSGGPRRLK
                     PHLDALGVGVWDPERIVVVTDHFVPASDAGSRQIVQLARDWVREQGIRHFYDEQGICH
                     VVTPRHGHLRPGLFAVGGDSHSCTGGAFGAYMFGIGATEMLGVVVTGEIWLRVPHTIA
                     MDWHGRLGPGVCAKDMMLHLCTRFGMDGGQYEAIEFCGEAVRALSMNERMTVSNMTAE
                     LGGQVGLIAPDATTAAWLRGVGVPEAALQDLEQWQTDAAAPLLAHHRLDAAALAPQVS
                     LPGSPAQAQEAGTLAGERLDIAYIGACTGAKLDDLRMAAQILRGRRTAAGVRLLVAPA
                     SAEDQRQAAAEGVLQVLQQAGAELLPNACGACAGYGAHTFAPDTTVISTTARNFTGRM
                     GAASSRVFLGSPYTVAASAVTGHITDPRELLA"
     gene            complement(13494..14462)
                     /locus_tag="PKN51_04910"
     CDS             complement(13494..14462)
                     /locus_tag="PKN51_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT84471.1"
                     /translation="MFNRRSWLWAGAAVALGAWMAPASAQDSRPITIVVGASPGGSTD
                     TLARTIAQSMGTTLKRTVIVDNKPGAGGNIAAQHVARSAPDGSTLLMSFTSHTINATL
                     FKKLPFNPVTDFTPITMVAQVPSVLVVRKSAPFDSVQGLIDYARKNPGKLSFAIGGQG
                     SSLHLAGEQFKQMTQTDIVNIPYKGTGPALTDLLADNTVDLMFASTINVLPHYKQGKI
                     KFLGVSSPQPLAQFAGLPAIAETVPGYQSQAWFGLFGPAKLPQAVTEALYGAVRVALD
                     SPEYRQRMQSEAATVPTMTPAQFADFIKKDVDFWGAIVKKSGAQVE"
     gene            complement(14758..15846)
                     /locus_tag="PKN51_04915"
     CDS             complement(14758..15846)
                     /locus_tag="PKN51_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNT84472.1"
                     /translation="MSAADPTRPTAPEWADPARQAAFERWLARLQPVHRLQPDSLRVA
                     SADASFRRYFRLDTEDGASRIVMDAPPAHENCAPFVRVAALMQAAGLHVPQILDWDEA
                     GGFMLMTDLGTRTLMEVLDRDRPEANRPRFAQAIDALLAWQQASRPGELPPYDEALLR
                     RELQLFPDWYLGRHRGVTLDAEQTATLERQFALIVARNLAAPQVHVHRDFMPRNLMVG
                     APEGPLGVLDFQDAVHGPITYDIASLMRDAFLSWDEEFVLDITVRYWERARKLGLLDF
                     EDWHGDFGSFWRAVEWMGLQRHLKVAGIFARLTLRDGKPRYLADAPRFIAYIRHTASR
                     YRELAPLLRLLDQVEGRTAVDGWAFGRV"
     gene            15973..18360
                     /gene="lptD"
                     /locus_tag="PKN51_04920"
     CDS             15973..18360
                     /gene="lptD"
                     /locus_tag="PKN51_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNT84473.1"
                     /translation="MPVSPPFLPIAAASRVRPRWALHPLLAGVLALLCGAATAQPGEP
                     LALRATPLLAEQPPSHVGAATTVYGQRVRGRIGLETVLEGQAELRQPGTVAQAERLSY
                     DQGSDIVTVSGGVRLNKAGDRYTAESGQLRVDAMEGFLLRPTYRFLASGAHGEAERLE
                     WLDRDRASVIQGSYTTCPRGGPDWVPDWIVRADRLDLDREDDEGRASGAVLEFKGVPV
                     LPVPALSFPISERRRSGWLPPTLGLDNKSGLDLSVPYYWNIAPHRDATFTPGLMSRRG
                     VDLAGEFRYLENDYQGRINANLTPNDRLRDGRNRWGYFLRHNGQHDSGIDGIGRVGLG
                     LNLNRVSDNDYWRDFSRRGLSLTTRLLANDGALSWSRGDLSLHARALKWQTLQDVAAP
                     IVPPYDRLPQLTARWGRSNDRGFDYAIEGDFTRFRADPFWTGQPNAERGFVQAQLARP
                     FTRPWGFFTPRVQLHASHYRFDGPISGGATSASRVLPTVSLDGGLVFERQAGFFGRDL
                     TQTLEPRLKYVYTPYRDQHRLPLYDTGAYDFNFATIWAENAFAGNDRIVDNNLVTGGL
                     TTRLLDPQTGAEVLRLGVAQRYRFSGQQVTLPGGLPTARGLSDLMLGASVNWDARWAF
                     DSVVQYNLDTRRSTRTTVQARYSPEKFHTVSAAYRHQRDLRSESLDIGWQWPLADLAW
                     RQGEPGGRSVGGGSSCQGKWYGVGRLNYSLQDRRLVEGILGFEYDAGCWIGRVVLEKL
                     NSSIHSSTKRIMFQVELIGLSRLGSNPLRALRNQIPRYQYLREETSSPSRFTDYE"
     gene            18353..19870
                     /locus_tag="PKN51_04925"
     CDS             18353..19870
                     /locus_tag="PKN51_04925"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT84474.1"
                     /translation="MSNSLPRRPSSWALLPLLLALAWPASGQAPSTAPAPAPSKASGK
                     KLRPAKRPAPTRAAPAPAPAPQDPAPAPAAAPASAPASAAPAARSASSTPREADYIVA
                     LVNSEPVTNHEVRARLARAQRQLAERGAPVPPADALRQEVLERLIAERAQLQYARETG
                     LKVDAAALTQAELSIARQNQLTSVAELHRRVEQEGIPVKDFQDDVRNQVLLARLRERE
                     IEPRLRVTDAEVEAFIREQTGVNPAAVDLNLAMILVAVPEGSSATELARLQARADEVA
                     RRARAGEDFAALALEFSDANNRGRDGGVLGLRPSERYPDLFVRSVQRARVGDIVGPVK
                     SEAGWHVLKLLERKQNKELPEVRIPQTHVQHILLRTGPAQSEQVARQRLADYKRRIQA
                     GQASFEQLAREHSQDASATDGGDLGWAPPGQFVPEFEQAMNNLDPGQISDPVTTRFGV
                     HLIRVEGRREQVLGAAEQRQLARNVLREKKAEEAFETWAREVRGRAYVELREPPR"
     assembly_gap    19980..20079
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20095..20865
                     /gene="rsmA"
                     /locus_tag="PKN51_04930"
     CDS             20095..20865
                     /gene="rsmA"
                     /locus_tag="PKN51_04930"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNT84475.1"
                     /translation="MKHIPRKRFGQHFLADPAIIDAIVRAIDPRPGQAMVEIGPGLAA
                     LTQPLVERLGRLTVIELDRDLAARLRAHPQLEVIESDVLKVDFGQIQQARAATKIRVC
                     GNLPYNISSPILFHLLPFAALIEDQHFMLQKEVIDRMVAAPATADYGRLSVILQWRYA
                     MENVLFVPPSAFDPPPKVDSAVVRMVPRSESAPVDADLLSELVRVAFSQRRKLLRHTL
                     GRWLSERGFAGAFDVQRRAEEVPVAEYVALAHELAAPR"
     gene            complement(20891..21847)
                     /gene="trxA"
                     /locus_tag="PKN51_04935"
     CDS             complement(20891..21847)
                     /gene="trxA"
                     /locus_tag="PKN51_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNT84476.1"
                     /translation="MIDVTVQNFEAEVIAASMHTPVLVDFWAPWCGPCKTLGPILEQL
                     EVEYAGRFKLVKIDSDQQQELAGAFGIRSIPTCILLKNGQPVDGFMGALPAGQLRQFL
                     DKHVPSEGALAAEAELDEAGALLEAGDTQAALAKLADALAQDPANDDARADYARLLIA
                     TGALSEAAATLEEPLAREPRPLRFDALRRWLDAIEFVQRDERGNWPLEQFDARIAQNK
                     RDFDTRFAKAQALLAEGQWTASMDELLEIILRDRKWNDEAARKTYVAILELLTPPPAK
                     PGAGDGGKSAGGIELTGKAAQQQDPQAELLSSYRRKLSMALN"
     gene            21901..22392
                     /gene="purE"
                     /locus_tag="PKN51_04940"
     CDS             21901..22392
                     /gene="purE"
                     /locus_tag="PKN51_04940"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNT84477.1"
                     /translation="MTTPLVGVVMGSSSDWDTMQHAVQILEQFGVPHEARVVSAHRMP
                     DDMFAYAEAAPQRGLRAIIAGAGGAAHLPGMLAAKTVVPVLGVPVASRHLQGVDSLHS
                     IVQMPKGVPVATFAIGAAGAANAALFAVALLATTDAALAQRLQAFRAEQTAAARAMTL
                     PPA"
     gene            22553..23755
                     /locus_tag="PKN51_04945"
     CDS             22553..23755
                     /locus_tag="PKN51_04945"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNT84478.1"
                     /translation="MSIPPPLLPGTLIDGRPATLGVLGGGQLGRMFVHAAQAMGYETA
                     VLDPDPQSPAGRVSQHFIQADYRDGAGLAELARRAHAISTEFENVPADSLAQLAGTRP
                     VAPAAAAVAIAQDRSAEKAHFARCAGAGGVAPAAHATITSEAELAAVPATLLPGILKT
                     ARLGYDGKGQARVATRAELAAAWQAMGRAPCVLEQRLALALECSVIVARGRDGQAVSL
                     PVQRNLHRDGILAVTEVHEGNLPPALASQAQAATIKIANQLDYVGVLCVEYFVLEGGA
                     LVVNEMAPRPHNSGHYSLDACDVSQFELQVRTLAGLPLRAPRQHSPAIMLNLLGDLWF
                     DAAGRPRTPPWDALLQLPGAHLHLYGKTEARRGRKMGHLTFTGASTEAVRASARQAAA
                     LLGMEAIG"
     gene            23752..24744
                     /locus_tag="PKN51_04950"
     CDS             23752..24744
                     /locus_tag="PKN51_04950"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNT84479.1"
                     /translation="MILDGARPEAIAQAVDALAAGRLVGLPTETVYGLAADAGNAAAV
                     RAIFATKGRPADHPLIVHVAGAAAVPAFASAVPDFAQRLIDACWPGPLTLILPRRPGV
                     ADAAAGGHPSIGLRCPAHPVARALLAACAARGIAGLAAPSANRFGRVSPTTAAHVAAE
                     FGDELLVLDGGACNVGIESTIIDATRGAPVLLRPGQLARADIERLAGRQLLSKEELMA
                     PDPQAPGTLSAHYAPTARLRLMDARELRAALQVLGAEARHIAVWARAPLTSASRQLLL
                     RRMPERADAAARELFAVLREFDAAGARLIWVETPPDDPAWDGVRDRLQRAAASA"
     gene            24907..25815
                     /locus_tag="PKN51_04955"
     CDS             24907..25815
                     /locus_tag="PKN51_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNT84480.1"
                     /translation="MNASWFRRAALALGCAGTLALLTACGSGSVVSDLAPKRFLTVGD
                     SFMDVGQSGKRYTVNDGSNNWVQELAAHYGVTVSAASAGGSGHAQGHARIAAADPIGG
                     APSVTTQIDTLLASTTLSDTDDVVLINGGMHDIVAAVEAGGLSDATTQAVKDAGKALA
                     TQVRRVVSAGGKHVVVTGVPLLGDSPWARARNQRDGINALSVAFNDALLSSIVDLGAH
                     VLYFDAAFFFNLMYGKPQNFDLANGLDPVCTTPDASTCTAATVASADYNRWLFADGLH
                     FSPGAQRKFASNDYAESAYRRFKERW"
     gene            25909..26991
                     /locus_tag="PKN51_04960"
     CDS             25909..26991
                     /locus_tag="PKN51_04960"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HNT84481.1"
                     /translation="MTPPRTHRIAVIAGDGIGQEVMPEALRAIDAVQRRFGFALQQTP
                     IAWASCDYYARTGQMMPDDWKAQLQGMDALLFGAVGWPATVPDHVSLWGSLLKFRREF
                     DQYINLRPVRLFDGVPCPLAGKKPGDIDFLVVRENTEGEYTNLGGVMYAGTEREIVVQ
                     ETVMSAFGADRVLKFAFEQAAARPRHQLDVATKSNGIAISMPWWDGRADAMAARYPQV
                     AVQKHHIDILTARFVLQPERFDVVVASNLFGDILSDLGPACTGTIGLAPSANLNPERR
                     FPSLFEPVHGSAPDIFGRGIANPVAMIWSAALMLQFLGEQAAHDAILRAIEQVLAAGP
                     RTPDLGGTASTVQMGQAIAARIEAGA"
     gene            complement(27102..>27312)
                     /locus_tag="PKN51_04965"
     CDS             complement(27102..>27312)
                     /locus_tag="PKN51_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84482.1"
                     /translation="AVIGVPGKTRRRRGLVPAGRSNAAWRAERGAGVRSVLTQEAKAI
                     AVAKPCTGAVRPKLTSGQLPFLGSA"
BASE COUNT         3969 a         9699 c         9543 g         4001 t
ORIGIN      
        1 agcgtgctca cccaggaggt gaaggccatc gcagccgcga aaccccgcat cgatgcggtc
       61 tgcccgacgt tgacaagcgg tcaactgccg tttctaggtt aaatatgcag catttggcgg
      121 caaggccggg cggtggcgac tccgccgcgc gcctgcgggc tatcgcaggc gctgtgaaca
      181 aaaacaacaa atcctgactt cctgatgcac ttggcgcggc ttcttgttgt gtcgcctcaa
      241 cagagtgcgc aaaatgtatt caaatgactc cgttggccgg tcgcctctgt ggctcggctt
      301 tgcacctgtt cgaggagaaa ccatgaagcg tacattgcgc atcagcgcgt tcgtgctggc
      361 ggcgggcctg gcgaccctgt ccggctgtgc caccaaggca cccattcccc tggccgagaa
      421 cttcgacctc accgtgcagc ccaaggtgcg ctcggccggt cactgggagc tgctgtccaa
      481 cgacgtggtc acgcagaccc tggcctccct gaagaacgcc caggtgccgg ccaacacacc
      541 gatggtggtg ggtctgccgg ccaacccctc ggcgttcgac cgcggttttc gcgaattcct
      601 gatcaccaag ctggtgcaaa gcggccagac ggtctacatg cagcccgacc aggcggcggc
      661 cgtcgcctgg cagacgcagg cctgcgccgc cacccccgcc tgccgtgacc aggacgcgct
      721 ggaggtcacc taccagaccc aggtggtgcg ccacgacagc ccgcgcccgc atttcgtgcc
      781 cggtcagttc accgtgctgg cggccggtct gatggccgcc tacggcctgc gcctggagca
      841 cctggacgcc aagctgatcg ccaccctggg cctggccgcc gcggccgact acggcgccag
      901 cgtgtatacc ggcggcccca ccaacaccga gctgatcctg accaccacgg tcagcaaccg
      961 cggccgcttc gtcagccgca agaccgacgt gtactacctg gaaaacgtcg acgcgccgct
     1021 gttcatgcgc tcggcgcccg gttacaaggc cgtcaacctg aaggtggtgt catgaggaag
     1081 ttcgcacccc ttgccctcac cctggcgctg gcctcgctgg ccggctgctc caccttcggt
     1141 gatgcggcgc cggtgcgccg cgccggcccc acctatgaaa cggcggccca caacgccttc
     1201 ctcgacagcg gccgcgcggc cgtcggcaag ctggtcgaag gcctgcctgc cgacaccctg
     1261 gctggcggcc cggtgctggt ggccacggtg gtcaacgtca acaacctgac gcgctcggcc
     1321 ccgctgggcc gcacgctgtc ggagcagtac gcctcgcaca tggcggcgca gggcttcaac
     1381 gtcaaggaag tcaagctgcg cggcgagctg ttcgtgcgcg aaggcacggg cgagctgctg
     1441 ctgtcgcgcg agctgcgcga aatcgcgcgc agccagaacg ccgccgtggt gctggtgggc
     1501 acctactcgc ccgcctccga ctacacctac gtcagcatca agctggtgcg caccgaagac
     1561 agccgcatcc tgcgcggcca cgactacgcg ctgcccaacg accgcgacgt gcagcgcctg
     1621 ctgcaggaac ccagcttccg ctgacggcgg cgcggcgcct gcgggcgcgc tcctgccgcc
     1681 ggccggccgg ccgccccatc ggcgcgctcg gtgcccgcag ccctgccgga ccacctgccg
     1741 gctgcgcctt cggcgcctgc gccgccgtgc gggttgatcg ggggttcggc atgccggcgg
     1801 gcgctttatc atccgtgcca gccgccgcga tcggcggggc gggcacccgg gcgcatcgtc
     1861 acggtcgccc tttcacagga gagcaaacga tgttgaacaa caaaaagtct tgcgccctgc
     1921 tgctggccgc tggtgcggcg ctgctgttga gcggctgcga aaccaccagc atgaagatgg
     1981 gcagcccgga ggccaagacc gtggccaccg gcagcgccgc cggcgccgcc acctcgggcg
     2041 agaacacggc gctggaaaag tgtgactcgc cgctgggcac ggtgtcgctg gtggaaaacc
     2101 agaccgccgg ctggtacacc atcctgaccg gtgaatacaa gctgccgccc accgccaacc
     2161 tgctgcgcct gctggtgcag cagtccaact gcttcgtggt ggtcgagcgc ggcgccgccg
     2221 gcatgcgcgc catgagccgc gagcgcgaac tgatgcagtc gggcgagatg cgcggcggca
     2281 gcaatttcgg caagggccag atggtggcct ccgactacgc gctgtcgccc gagatcgtgt
     2341 tcagcgccga caacgccggc ggcatcggcg gcgtgctggg cggcctgatc ggtggcggca
     2401 gcggccgcgc cgtggcggcc atcggtggcg gcctgcagac gcgtgaggcc agcgccatgc
     2461 tgaccctggt ggacaaccgc tccggcgtgc aggtggcggc gtcggaaggc agcgcctcca
     2521 agaccgacct caacctgttc ggcggcctgt tcggcggccg tgccggtgcc ggcctgggcg
     2581 gctacaccaa cacgccgcag ggcaaggtca tcagcgccgc cttcatggac gccttcaacc
     2641 agatggtgcg cagcctgcgc aactaccgcg cgcaaagcgt gcaaggccag ggcctgggcg
     2701 gcggcggtcg cctgggtgtg gacggcgggg ccgcgcccag ccagacctac gtacccgaaa
     2761 acgcacccaa gagcgcgccc agcaagcgcg caccgacgcg ccggcgctga gcgccggtca
     2821 caccggcatg gccgggtgcg ggtggcgcgg ggcacccgga cgaacccgcg catgctacga
     2881 taatcatagc tgccggcgct tgccatacgg gcgctggtgg cttaaggttc gattgcccga
     2941 tgcgggctcg ctttcagatg ggccaatggc ggatgacctg gcggcgcttc gatcaccaag
     3001 cggtcatcgg tctggggcca tgtctctgaa cgcgaagccg tgtgaacccg tggcgcgcag
     3061 gccgggcccg ccgccgcgcg ctgccgcccg ttgcccatga gcctgcttga gttgatccgc
     3121 cgccgccgcg ccagcctgcc cgactgggtg caggaatggc tggacgtgct ggtgccggcg
     3181 ctgcaggtgg cgctgatcgt gctggccgcc tggctgctgc actggctgct caagcgcctg
     3241 gtgctgcgcc tggcgcgcct gtacgccctg ccgccggcca tcgtgctgcc gctgcgcggg
     3301 gtgctgcgct ggctgatcat cgtcgccacg gcgctgctgg tgctgggccg gctgggggtg
     3361 tcggccgagg tgctgtggac cgccttcacc ggctttgccg cggtggcggc ggtggccttc
     3421 tttgccgcct ggagcgtgct gtccaacctg ttctgcgcgc tgctcatcat ggtggcgcag
     3481 ccgtttcggc tgggcgacga cctggaggtg ctggacagcg ccgaaaaacc cggcgccaag
     3541 ggccgtgtgg tggacatcaa cctgctctac accacgttgc aggaaaccga tggcggcggg
     3601 cggctgctgc agattcccaa cgcgctgttc tttcagcgcg tggtgcggcg ctggcgcggt
     3661 ggcatggcgg ccgccgccgc cgacccgtcg gcagcagcgc cggccggtca cgaaccaggc
     3721 tgagcgccgg ccggtcacga accaggctga gcgccggcct acggtgccag caccttgccc
     3781 gggttcagga tgttgtgcgg gtccagcgcc tgcttgatgg tgcgcatcag cgccaccgcc
     3841 ccggcgccgg cttcgtcgag cagaaagccc atcttgtgca ggccgacgcc gtgctcgccg
     3901 gtgcaggtgc cgcccagggc cagcgcgcgt tcgaccacct gatggttgag ctgttcggcg
     3961 cgggcgcgct cggcgtcgtc gtcggggtcg atcaggtagc ccatgtggaa gttgccgtcg
     4021 cccacgtggc cgaccaggaa gtaggggatg ccgctggcgt cggcttcctg cacgctggcc
     4081 agcacggcgt cggccaggcg cgagatcggc acgcagacgt cggtggtgat gcagcggcag
     4141 ccggggcggc tttgtacgcc ggcgaaatag gccttgtgcc gcgccgtcca caggcgggtg
     4201 cgctcctcgg gcgtgtcggc ccactcgaag ccggcgccgc cgtgctcgcc cgcgatggcc
     4261 tgcaccgttt cggcctgctc gcgcacgccg gcgggcgagc cgtgaaactc catcagcagc
     4321 atcggtgcct cgcgcaggct gagcttgctg tgctggttga cggcgcgcac ggtgcgcgcg
     4381 tccagcagtt cgacgcgggc gatcggcaca cccagctgga tgatctggat cacggtgccc
     4441 accgcttcgg cgatgctggg gaaggaacag atcgccgccg acaccgcctc gggcagcggg
     4501 tacaggcgca gggtgatttc ggtgatgacg cccagcgtgc cttcgctgcc gacgaacagg
     4561 cgcgtcaggt cgtagccggc gctgctcttg cgcgcgcggg tgccggtgcg gatcacctcg
     4621 ccgctggcgg tgaccacttc cagcgccagc acgttctcgc gcatggtgcc gtagcgcacg
     4681 gcgttggtgc cgctggcgcg ggtggcgctc atgccgccca gggtggcgtt ggcgcccggg
     4741 tcgatgggaa agaacaggcc ggtgtcgcgg atctcggcgt tgagctgctc gcgcgtcacg
     4801 cccggctgca ccgtcaccag cagatcttcg gcctggatcg acagcacccg gttcatgcgc
     4861 gacagatcga cgctgatgcc gccctgcacc gccagcagat gcccttccag cgatgagccg
     4921 acgccgaacg ggatcaccgg cacctggtgc tgcgcgcaca ggcgcaccag atcgctcacg
     4981 tcctgcgtgc tttcggcaaa caccacggcg gccggcggcg gcacggtggt gaaggccgac
     5041 tcgtcacggc cgtgctgctc gcgcaccgcc tgggcgtcgg aaaactgcgc gccgaagcgg
     5101 ctcctgaggg cgtcgatcag cacgcccggc accgggcgcg gctggatccg gggctggacg
     5161 gggtggacgg cggcgggggc gttcatgggc aatctcctgg cgtgcaggca cagtgtaagc
     5221 cgctctgccg gcgcgtgaag aagctattat attgatagct gtcggcgctt gttggacggg
     5281 cgccgaaggc gctttcgatc ctcaagactg gcgcggcgcc agcaccgcca tggtcaggcg
     5341 cgagacgcag gtgagttcgc cggcgtcgtt gtgcatgtcg atctgccaca cctgcgtggt
     5401 gcggccgatg tgcaccggcc gcgcggtgcc ggtcacccag ccacgggtgg cggcgcgcag
     5461 gtggttggcg ttgatgtcca gcccgacggc gcgccagccc tcggggcagg cgtagacggc
     5521 accgcaggag cccagcgttt cggccagcac cacgctgacg ccgccgtgca acaggccgta
     5581 gggctggcgc gtgtgcgcgc cgaccggcac gcgcgcgcgc aggaagtcgt caccgacctc
     5641 gatgaactcg atgcccaggt gcgacaccgc ggtgtcgcgg ctgatggtgt tcagaatctc
     5701 gggcgagacg gcttgcttcc agatcggcat ggcgtgacag gtgaagaaca gggacgaagc
     5761 ggccaagcct accgcagccg ggccgctacc atcgcgccat gcctgatcgt tacgccgtca
     5821 tcggccaccc catcgcccac agcaagtcgc ccttgatcca cggcctgttc gcccaggcca
     5881 ccgggcagga catcgtgtac gaggccatcg acggcggcgc ggcgccaggc ggcttccagc
     5941 gtgcgctggc cgactttcgc gccgccggcg ggcgcggcct gaacgtcacc ctgccgttca
     6001 agctggcggc gctggccgtc gccgacctgg ccagcgagga cgcgcgcctg gctggcgccg
     6061 ccaatgcgct gcgcttcgag gacgggcgca tcgaggcgcg caacttcgac ggcatcggtc
     6121 tggtgcgcga catcgaggtc aacctgggtc tgccgctggc cggtcggcgc gtgctgctgc
     6181 tgggtgccgg cggcgccacg cgcggcgcgg tgcggccgct ggcgcgcgcc ggcgcggcgc
     6241 gcatcgtcat cgccaaccgc accgccgaca aggcccggac cctggcggcc gagctggcgc
     6301 cacatctgga cggcgtgccg ctggtgggcg gagggctggc ggcgctggcc accggcgaag
     6361 cgttcgaggt ggtcatcaac gccacctcgg ccagcctgag cggcgccgcg cccgagctgc
     6421 cggcgtgcgc gttcgcgccc ggcgccctgg cctacgacat ggtctacggc cagggcctga
     6481 ccccgttcct gcggctggcg cgcgcagccg gctgcgcgcg cctggccgac ggcgtgggca
     6541 tgctggtcga gcaggcggcc gaggcctttg catggtggcg cggcgtgcgc cccgacacgc
     6601 gcgaggcgct ggcgcgcctg acggtgccgc tggcctgatt cacaatcgcg ccatgaacct
     6661 cctcgtcctg cacggcatca acctcaacat gttcggccag cgcgacccgg cgcactatgg
     6721 cagcgccacg ctggccgacg tcgacgccgc gctgcagcgg ctggccggcg agctgagcgc
     6781 acgcgtcgag tgcttccaga ccaaccacga aggcgcgctt tgcgagcgca tccaccaggc
     6841 gcaccgcgac gccgtcgacg gcgtgctgat caacgccggc gcctggacgc acaccagcat
     6901 cgccatccgc gacgcgctgg ccatcctcca gtgcccgatc gtcgaagtcc acatgtccaa
     6961 catccacgcc cgcgagccgt ttcgccacca ctcgttcatc gccgggctgg cgcgcggcca
     7021 gatctgcggc tttggtgtcg attcctacct tctggggctg cgcgccctgg tggggcaagc
     7081 gcaagcagct atcaaaaagt gagaaatcag ccatgggcaa gcgcctgacg cagatcgcca
     7141 cccgcaccgg cgacgacggc accaccggcc tgggcgacaa cacccgggtg tccaaggcca
     7201 gcggccgccc gcaggccatg ggcgatgtgg acgagctcaa cagccacatc ggcctgctgc
     7261 tgtgcgaagc gctgccgccg gccgtgcgcg agctgctgat cgacatccag caccagttgt
     7321 tcaacctggg cggcgagctg gccatgcccg gcttcgagct gctcaaggac gaggcgctgg
     7381 cgcaactgga cgcggcactg gcgcagcaca acgccgcgct gccgcggctg gaggaattca
     7441 ttctgccggc cggcacccgg gccgcctgcc aggcccatgt ctgccgcacc gtggcgcgcc
     7501 gcgccgagcg cgccgtggtg gcgctcggcc agcaggagcc gctgcgcgcc gcgccgcgcc
     7561 agtacctgaa ccgcctgtcg gacctactgt tcgtgctctc gcgcgtgctc aaccgctacc
     7621 ggcccgacgg cagcgtgggc gacgaggtgt actggaaaag cgagcggctg gcgcgcgcgt
     7681 cgaccgaggg ctgaacgacc ccggttcagg gcagcgcttt cagccaggcc agcaccggct
     7741 cggccgtctg ctcgaagtcg ccgtggccgc cggaatacac caggtactgg ctggccgggt
     7801 ggggcggcgc acgccggaat gcgaactcct cgccaccgcg gtagatcggg tcggccgtgc
     7861 ccaccaccca cagcaggggc accggcttct tgaaggccgc cgcactggcc ggcatgtggc
     7921 ccagcccctc ggggtcgaag tagctccaca gcacctcggc cgacatgcgc acgctcttgc
     7981 gctggccctg gttgaggtct tccatcgtca gggtctcgct gccctggccg gcctgcacca
     8041 actggcgcgc cttgtccacc gcgtccttgc caatgccgcg cgcatacatc atcgcgggcg
     8101 aatggccggg cgccaccgcc accacgccat cggcgtcgcc cacccgggcc atgtaggcca
     8161 tggcggcgtt ggcgccgaag ctgtggccgc ccaccagcac gcggcggtag ccctgatctc
     8221 gcagcgcctt cacctcggcc gccatatcgt cgagcgcgcg cgaatagggc gcgtcgtagc
     8281 cgcggcgcag cgaccatggc gcctcggcca cgcgcacctt gcagtatggc tccaggcggc
     8341 gcgtgaagaa tcccacgtac tgggtgttgc cccatttgcc gtgtacgaac accagcgcac
     8401 aggccttgtc gtcctgcgcc catgccgggc cgttcagcgt cggtgccagc agggccgaca
     8461 gcgtcagcac ctggcgacgg gtgggcatct cggtcatcgt ggttctcctt gttggcggtg
     8521 gcttgcgccg aggcgatggc tcaggcgcgc cggcgcagca gcgcgtgcag cagcaccgcg
     8581 ccaaaggtgg cggtgccgat gccgccgagg gcgaagctgc cgaacttcag cgtgaagtcg
     8641 cccgtgccca ggatcagggt gatggcggcg gtcagcaggt tggccgggtc gccgaagtcg
     8701 accttgtttt ccacccagat gcgcgcaccg gcgatggtga tcagaccgaa caccacgatg
     8761 ctgacgccgc ccatcaccgg cagcggaatg gcctggatca gggcgccgaa cttggggctg
     8821 aagcccagcg ccagcgccat cagcgccgcc accacgaaca ccgcggtgga atagatgcgc
     8881 gtggccgcca tgacgccgat gttttcggca taggtggtga cgccagtgcc gccggcggca
     8941 ccgctgatca tggtggccac accgtcggcg gtgaaggcgc ggccgatgta ggggtcgagg
     9001 ttgcgcccgg tcatggccgc caccgccttc aggtggccca ggttctcggc caccaggatc
     9061 agcgccaccg gggcgatcag cagcatggcg ttgccttcga agaccggcgc ggtgaagcgc
     9121 ggcagcccca cccaggcggc gctggcgatg ccggaaaaat ccatcggctt gcccagcccc
     9181 agcccattgg ccagcagcac gtagatcacg ctggcgatcg ccagcccgac caggatcagc
     9241 aggcgctgca ccatgccgcg cgcaaacacc gccaccagcg ccacgctgac gaaggtgacc
     9301 agctgcatcc agctgtcgaa gttgctggcc gccatgttct tgaccgggat ggccgccagg
     9361 ttcagaccga tcaccgccac caccgcgccg gtgaccaccg gcggcatcag gcgctcgatc
     9421 cagcgcgtgc cgacggcgtt gaccagcgcg ccgatcagcg cgtacagcag gccgcagacg
     9481 atgatggcgc ccagcgccac ggcgatgttg gggttgggcc cctggccggc gtagccggtg
     9541 gcggcgatca ccacgccgat gaaggcaaac gacgaaccca ggtagctggg cacccggccg
     9601 ccggtgatga agtagaacag cagcgtgccg acgccgctca tgaagatcgc caggttgggg
     9661 tcgaacccca tcaggatcgg cgccagcacg gtggctccga acatggcaat cacgtgctgc
     9721 acccccatgg cgaccgtctg cggccagggc aggcgctcgt cgatgtgcac cacaccgcct
     9781 gccgccagtt gagacgcggg tttttcctgc cagttgaaca ggctcatggg ctccactcct
     9841 ttgctttggg gttgtcaggg ggcgcgcgcc ggcgcgaccg cccgcgatgg tagtgcagcg
     9901 cgggcccggc tgccggcggc gcctgcggcg catcgggcgg cgggcggcgg gcggcgggcg
     9961 gcgggcggcg ggccgctgcg ccgtcagtgc gtgatccaca ccgtggtcga tcccccggtg
    10021 cgcgacaggc gcatgcggta gtggtagtgg tcgggtgcgt aatggcctat caggtattgc
    10081 agtggccggc cctgggcatc ggccaccacg cggttgacgc gcagcagcgc ggcgccgggg
    10141 gcgatgcgca gcagttcggc cacctccgcc ggggcgacca cggccatcag ctcctgctca
    10201 ccctgggcaa aggcgtggcc ggcctgttcg atggcttcga gcagcggcat gtcgtgcagc
    10261 atctgcgggg cgatgccttc ggccaccgcg gccggcaggt aggcctcggt gcacagcagc
    10321 ggtgtgcggt gtgacttgcg cacgcgcacc acgcgcatca cgcggctgcc ggccggcagc
    10381 tgcagggcct gggccacggc atcgggcgct gccatgtatt gcgtcgacac cagcgtggtg
    10441 cgcgtgcgct ggccgaagcg caggatgttc tccagcaggc cgttgcggat cttcgaatgc
    10501 tcgccggtgc tgggctcggc caccggcagc gtgccctggc cgggcaggcg ggtgatgcag
    10561 ccttcctcct gcaggcgcgc aagggccgcg cgcacggtgc tgcgcgccac gccgaattcg
    10621 cgcgtcagtt cgcgttcgcc cggcagcggt atgcccacgg ggtaactgcc gtcgcgcagg
    10681 cgctggcgca aggtctgggc gatctgctga aacaggggct cggtcgtgta tgcggggcgc
    10741 gtcaagacgg tctcctggcc gggcacgcca tgcggccggt caggtgatta tcggcgtcgg
    10801 ggtcagtcgt agcgctggcc cagcgccagc agctcgggcg tgccgatgac ggcgttgagc
    10861 gcatcgaagt ccagcatgcg atcgcgccag ggcagggtgc tgccgtgggt gtgcaggctg
    10921 gccagatagc cttgcagcgc gtgcgtgagg gcgcgcaccg tgccgcccgg gaagatcacc
    10981 aggcggtagc cgatctcggc gagctcctgc gcgctggtca cgggtgtcat gccgccttcg
    11041 accatgttgg ccatcagcgg gcacagtgcg cccaggacct ggttgagctg ctgcatctgg
    11101 tcgcggctgc gcacggcctc gacgaacagc aggtcggcgc cggcctcggc gtagcgggca
    11161 gcgcgctcca gcgcggcgtc aaagccctcc accgccacgg cgtcggtgcg cgcgatgatc
    11221 aacgtgtcgt cgtcgcggcg cgcgtccacg gcggcgcgca gcttgccgac catctcgcga
    11281 gtggcaatca cggacttgcc ttgcaggtgg ccgcagcgct tggggctggt ctggtcttcg
    11341 agctggatgg ccgaggcgcc ggcgcgctcg agctggcgca cgctgcgcat cacgttgagc
    11401 gcgttgccaa agccggtgtc ggcatcgacg atgagtggca gctccacgcg ctcgcggatg
    11461 cagctggtga cctgggctac gtcgtccagg cccagcagac cgatgtcgga gcggcccaga
    11521 cgcgtgtagg cgatgctggc gcccgacaag tacgcggcgg gaaagcccgc ctgctgcacc
    11581 agcagggcgg tcagggcgtc gtacacgccc ggggcgatgg tgatgccggg cgcggccagg
    11641 cgttgcttca ggctcatggt gcggtgcgcg tgtgttgcgg gcgctggcgc tcgatgcgcc
    11701 ggcgcaggtg tggcaccagg ccgccatcgg ccaccagtgc cagcaggtgg gtggggatgg
    11761 gctggcagga cagctgccgt gcatcgggca gttgcagccg cgcctgcgcc agatccagca
    11821 ccgccggcgt gcccggcgca atgccccagg cgggcgccgg catgtcggcc gggccgagca
    11881 gcagcggcag acccaggttg aagccgttgc ggtagaacag cccggcaaac gagcgtgcga
    11941 tcacgcattg cacgcccagg tggcgcagca cttcgacggc ctgctcgcgc gaggagccgg
    12001 tgccgaagtt gcgcccggcg atgatcaggt cgccggcgcg cacctcggcg gcaaagtgcg
    12061 gcagcacgtc ctccaggcaa tgccgggcga tgtcctcgat gccgaatttc atgtacttgc
    12121 ccggcgccag gtagtcggtg ttgatgtcgt cacccagcac ccagcagcgc ccgggccggg
    12181 gctgggcgtt gaccggtgcg ggtgaggtgg agtggttcat gccagcagct cccgtggatc
    12241 ggtgatatgg ccggtgacgg ccgaggccgc caccgtatag ggcgagccca ggaatacgcg
    12301 cgaggatgca gcacccatgc gccccgtgaa gttgcgcgcc gtcgtgctga tgacggtggt
    12361 gtcgggcgca aaggtgtggg cgccgtagcc ggcgcaggcg ccgcaggcgt tgggcagcag
    12421 ctcggcgccg gcctgctgca gcacctgcaa cacgccttcg gcggcggcct ggcgctgatc
    12481 ttcggcgctg gcgggtgcca ccagaaggcg cacgccggcg gcggtgcggc gcccgcgcag
    12541 gatctgggcg gccatgcgca ggtcatcgag cttggcaccc gtgcaggcgc cgatgtaggc
    12601 gatgtccagg cgctcgccgg ccagcgtgcc ggcctcctgt gcctgggccg gcgagccggg
    12661 cagggacacc tggggcgcca gcgccgccgc gtccaggcgg tggtgcgcca gcagcggcgc
    12721 cgccgcgtcg gtctgccact gttccaggtc ttgcagcgcg gcctcgggca cgcccacgcc
    12781 gcgcagccat gcggccgtgg tggcgtcggg cgcaatcagg ccgacctgac cgccgagctc
    12841 ggccgtcatg ttcgacaccg tcatgcgctc gttcatggac agggcgcgca cggcctcgcc
    12901 gcagaattcg atcgcctcgt actgtccgcc atccatgccg aagcgcgtgc acaggtgcag
    12961 catcatgtcc ttggcgcaca cgccgggccc caggcgccca tgccagtcca tggcaatggt
    13021 gtgcggcacg cgcagccaga tctcgccggt caccaccacg cccagcatct cggtggcacc
    13081 aatgccgaac atgtaggcac cgaacgcgcc gccggtgcag gaatggctgt cgccgccaac
    13141 ggcaaacagc cccgggcgca gatggccgtg gcgcggcgtg accacgtggc agatgccctg
    13201 ctcgtcgtag aagtggcgta tgccctgctc gcgcacccag tcgcgcgcca gctgcacgat
    13261 ctggcggctg ccggcgtcgc tggcgggcac gaaatggtcg gtgacgacga cgatgcgctc
    13321 cggatcccag acgcccacgc ccagcgcgtc cagatggggc ttcaggcgtc gcgggccgcc
    13381 cgagtcgtgc atcatggcca gatcgacgcg gcaggtgacg atctggccgg gctgcacctg
    13441 tgtctggccg caggcgcgcg ccaccagttt ctgggccagg gtagcgggca tggtcactcc
    13501 acctgcgcgc cggacttctt cacgatggcg ccccagaagt ccacgtcctt cttgatgaag
    13561 tcggcaaact gcgccggcgt catggtcggt acggtggccg cctcggactg catgcgctgg
    13621 cggtattcgg gcgaatccag cgccacgcgc acggcgccat acagcgcttc ggtcacggcc
    13681 tgtggcagct tggccgggcc aaacaggccg aaccaggcct gcgactggta gcccggcacc
    13741 gtctccgcaa tcgccggcag gccggcaaac tgcgccagcg gctgcgggct gctgacaccc
    13801 aggaacttga tcttgccctg cttgtagtgc ggcagtacgt tgatggtgct ggcaaacatc
    13861 agatccacgg tgttgtcggc cagcaggtcg gtgagcgccg ggcccgtgcc cttgtagggg
    13921 atgttcacga tgtcggtctg cgtcatctgc ttgaactgct cgccggccag gtgcagcgac
    13981 gagccctggc caccgatggc aaagctgagc ttgccggggt tcttgcgcgc atagtcgatc
    14041 aggccctgca cactgtcgaa cggtgcgctc ttgcgcacca cgagcacgct gggcacctgg
    14101 gccaccatgg tgatgggggt gaagtccgtc accgggttga agggcagttt cttgaacagc
    14161 gtggcattga tggtgtggct ggtgaagctc atcagcagcg tggagccatc gggcgcgctg
    14221 cgcgccacat gctgcgcggc gatgttgccg ccggcgccag gcttgttgtc cacgatgacg
    14281 gtgcgtttga gcgtggtgcc catgctttgg gcgatggtgc gcgccagggt gtcggtggag
    14341 ccgcccggcg aggcgccgac gacgatggtg atggggcggc tgtcctgcgc gctggccggc
    14401 gccatccacg cacccagcgc gacggctgcg ccagcccata gccacgatct cctgttgaac
    14461 atgtcggtct ccttgtgcac cagaggttga agggggagct tccgatgctg ggcacgcgtg
    14521 gtctgcgctt caataggacc agctggttcg ctggggtttg caccgtggcg ggcctcatga
    14581 agggatctgc ggcgccgatg gccccgcatg gcacccgact ggcagcccag gagcatgccg
    14641 acccgccccg tgtgctacgt aaacaatagc tgtttgcgct gacccggcgg gcgttggggc
    14701 tcgatttgtt tggattgttg cggccggctt cgagcgccgg catcgccgct tggggcgtca
    14761 gacccgccca aacgcccagc catcgacggc ggtgcgcccc tcgacctggt cgagcagccg
    14821 cagcagcggc gccagttcgc ggtagcgcga tgcggtgtgg cggatgtagg cgatgaagcg
    14881 tggcgcgtcc gccaggtacc tgggcttgcc gtcgcgcagg gtcaggcggg cgaagatgcc
    14941 ggccactttc aggtggcgct gcaggcccat ccattcgacc gcgcgccaga agctgccgaa
    15001 gtcgccgtgc cagtcctcga agtccagcag ccccagcttg cgcgcgcgct cccagtagcg
    15061 caccgtgatg tcgagcacga actcctcgtc ccagctcagg aaggcgtcgc gcatcaggct
    15121 ggcgatgtcg taggtgatgg ggccgtgcac cgcgtcctgg aagtccagca cacccagcgg
    15181 gccttcgggc gcgcccacca tcaggttgcg cggcatgaag tcgcggtgca catggacctg
    15241 cggcgcggcc aggttgcgcg ccacgatcag cgcgaattgg cgttccaggg tggcggtctg
    15301 ttcagcgtcc agcgtcacgc cgcggtgccg gcccagatac cagtcgggaa acagctgcag
    15361 ctcacgccgc agcagcgcct cgtcgtaggg cggcagctcg ccgggccggc tggcctgctg
    15421 ccaggccagc agcgcgtcga tggcttgcgc gaagcggggg cggttggcct cgggccggtc
    15481 gcggtcgagc acctccatca gggtgcgcgt gcccaggtcg gtcatgagca tgaagccgcc
    15541 ggcctcgtcc cagtccagga tctgcggcac atgcaggccg gcggcctgca tcagcgcggc
    15601 cacgcggacg aagggcgcgc agttctcgtg cgcgggcggc gcgtccatga cgatgcggct
    15661 ggcgccgtct tcggtgtcca ggcggaagta acgccggaag ctggcgtcgg ccgaggccac
    15721 gcgcaggctg tcgggttgca gccggtgcac cggctgcagg cgcgccagcc agcgctcgaa
    15781 tgcggcctgg cgggcggggt cggcccattc gggggcggtg gggcgggtcg gatcggcggc
    15841 gctcatggat aatcgattct aggaggctgt cggacttgga gcgccgaagc cgagcgccag
    15901 cgccagccgc ctccgaaaat gcgcccatcc ggctcacggc ttggcgggcg cgtcgatccg
    15961 acggttgttg ccgtgcctgt ttcacctcca ttcctgccca ttgccgccgc ctcgcgtgtc
    16021 cgaccgcggt gggcgctgca cccgctgctg gcgggcgtgc tggcgctgct gtgcggtgcg
    16081 gcgacggcgc agcctggcga gccgctggcg ctgcgcgcca cgccgctgct ggcggagcag
    16141 ccgccctccc atgtcggcgc ggcgaccacc gtgtacggcc agcgcgtgcg tggccgcatc
    16201 ggtctggaga cggtgctgga aggccaggcc gagttgcgcc agcccggcac cgtggcgcag
    16261 gccgaacggc tcagctacga ccagggcagc gacatcgtca ccgtcagcgg cggcgtgcgg
    16321 ctgaacaagg cgggcgaccg ctacaccgcc gaatccggcc agctgcgcgt ggacgccatg
    16381 gaaggctttc tgctgcggcc cacctaccgc tttctggcca gcggcgcgca cggcgaggcc
    16441 gagcgcctgg aatggctgga ccgcgatcgc gccagcgtca ttcagggcag ctacaccacc
    16501 tgcccgcgcg gggggccgga ctgggtgccc gactggatcg tgcgcgccga ccgcctggac
    16561 ctcgaccgcg aggacgacga agggcgcgcc agcggcgccg tgctggaatt caagggcgtg
    16621 ccggtgctgc cggtgccggc gctgagcttt ccgatcagcg agcggcgtcg ctccggctgg
    16681 ctgccgccca cgctggggct ggacaacaag agcgggctgg atctgtccgt gccgtactac
    16741 tggaacatcg caccccaccg cgatgccacc ttcacgcccg gcctgatgtc gcgccgcggg
    16801 gtcgatctgg cgggcgagtt ccggtatctg gaaaacgact accagggccg catcaacgcc
    16861 aacctgacgc ccaacgaccg cctgcgtgac ggccgcaacc gctggggcta cttcctgcgc
    16921 cacaacggcc agcacgacag cggcatcgac ggcatcggcc gcgtcgggct ggggctcaac
    16981 ctcaaccgcg tcagcgacaa cgactactgg cgcgacttct cgcgccgcgg cctgtcgctg
    17041 accacccgcc tgctggccaa cgacggcgcg ctgtcgtggt cgcgcggcga tctgtcgctc
    17101 cacgcccggg cgctcaagtg gcagaccctg caggacgtgg ccgcccccat cgtgccgccc
    17161 tacgaccgcc tgccgcaact gacggcgcgc tggggacgca gcaacgaccg cggcttcgac
    17221 tacgcgatcg agggcgactt cacgcgcttt cgcgccgacc cgttctggac cggccagccc
    17281 aacgccgagc gcggcttcgt gcaggcgcag ctggcgcggc ccttcacccg gccctggggc
    17341 tttttcacgc cccgggtgca gctgcacgcc agccactacc ggttcgacgg gccgatcagc
    17401 ggcggcgcca cctcggccag ccgcgtgctg cccaccgtca gcctcgacgg cggcctggtg
    17461 ttcgagcgcc aggccggctt tttcggccgc gacctcacgc agacgctgga gccgcgcctg
    17521 aagtacgtct acacgcccta ccgcgaccag caccggctgc cgctgtacga caccggcgcc
    17581 tacgacttca acttcgccac catctgggcc gagaacgcct ttgccggcaa cgaccgcatc
    17641 gtcgacaaca acctggtcac cggcggcctg accacgcgcc tgctcgaccc gcagaccggc
    17701 gccgaggtgc tgcgcctggg cgtggcccag cgctaccgct tttcaggcca gcaggtcacc
    17761 ctgccgggcg gcctgccaac cgcgcgcggg ctgtccgacc tgatgctggg cgcctccgtc
    17821 aactgggacg cgcgctgggc cttcgacagc gtggtgcagt acaacctgga cacgcgccgc
    17881 tccacccgca ccacggtgca ggcgcgctac tcgccggaaa agttccacac cgtcagcgcc
    17941 gcctaccgcc accagcgcga tctgcgcagc gagtcgctcg acatcggctg gcagtggccg
    18001 ctggccgacc tggcctggcg ccagggcgag ccgggcgggc gcagtgtcgg cggcggcagc
    18061 agctgccagg gcaagtggta cggcgtcggg cggctgaact acagcctgca ggaccggcgc
    18121 ctggtcgaag gcatcctggg cttcgaatac gatgccggct gctggatcgg ccgcgtggtg
    18181 ctggaaaagc tcaacagctc gatccactcc tccaccaaac gcatcatgtt ccaggtcgaa
    18241 ctgatcggcc tgtcgcgcct gggcagcaac ccgctgcgtg cgctgcgcaa ccagattccg
    18301 cgctaccagt acctgcgcga agaaacctcc tcacccagcc gcttcaccga ctatgagtaa
    18361 cagcctgccc cgccgcccgt cctcctgggc gttgttgccg ctgctgctgg cgctggcctg
    18421 gccggccagt ggtcaggccc cctcgaccgc cccggcgccc gccccgtcca aggccagcgg
    18481 caagaagctg cgtccggcca agcgcccggc gccgacccgg gccgcgccgg cgccggcccc
    18541 ggccccgcag gatccggcgc ccgctcccgc ggccgcgcca gccagcgccc ccgccagcgc
    18601 cgcgcccgcg gcgcgcagcg ccagcagcac gccgcgcgag gccgattaca tcgtcgccct
    18661 ggtcaacagc gagccggtca ccaaccacga ggtgcgcgcc cgtctggcac gtgcccagcg
    18721 ccagctcgcc gagcgcggtg ccccggtgcc gccggccgat gcgctgcgcc aggaggtgct
    18781 cgagcgcctg atcgccgagc gcgcgcagct gcagtacgcg cgcgaaaccg ggttgaaggt
    18841 cgacgccgcg gcgctgaccc aggccgaact gtcgatcgcg cgccagaacc agctgaccag
    18901 cgtggccgag ctgcaccggc gcgtcgagca ggaaggcatt ccggtgaagg acttccagga
    18961 cgacgtgcgc aaccaggtgc tgctggcgcg cctgcgcgag cgcgagatcg agcccaggct
    19021 gcgcgtcacc gacgccgagg tggaagcctt catccgcgag cagaccggcg tcaacccggc
    19081 cgccgtcgac ctgaacctgg ccatgatcct ggtggccgtg cccgaaggca gcagcgccac
    19141 cgagctggcg cggctgcagg cgcgcgccga cgaagtggcg cgccgcgccc gcgccggcga
    19201 ggacttcgcc gcgctggcgc tggagttttc cgatgccaac aaccgcggcc gcgacggcgg
    19261 ggtgctgggc ctgcgcccgt cggagcgcta ccccgatctg ttcgtgcgca gcgtgcagcg
    19321 cgcgcgcgtg ggcgacatcg tcgggccggt caagtccgaa gccggctggc acgtcctcaa
    19381 gctgctggag cgcaagcaga acaaggagct gcccgaagtg cgcattccgc agacccacgt
    19441 gcagcacatc ctgctgcgca ccgggccggc gcagagcgag caggtggcgc gccagcgcct
    19501 ggccgactac aagcgccgca tccaggccgg acaggccagc ttcgagcagc tggcgcgcga
    19561 acattcgcag gacgccagcg ccaccgacgg cggcgatctg ggctgggcgc cgccggggca
    19621 gttcgtgccg gagttcgagc aggccatgaa caacctcgac ccgggccaga tcagcgaccc
    19681 ggtcaccacc cgctttggcg tgcacctgat ccgcgtcgaa ggccggcgcg aacaggtgct
    19741 gggtgcggcc gagcaacgcc agctggcgcg caacgtgctg cgcgagaaaa aggccgagga
    19801 agccttcgag acctgggccc gggaagtgcg cgggcgcgcc tacgtcgagc tgcgcgagcc
    19861 gccgcgatag ggcccgagcc ggcgttcgac gtgtgaagaa gaggttgagc gttcggcgtt
    19921 gagcgttcag cgtgaatacc ggagctttcg cggccacgtc catctgtcaa cgctcaacgn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt caacgctcaa cggcatgaag
    20101 cacatccccc gcaaacgctt cggccagcac tttctggccg acccggcgat catcgacgcc
    20161 atcgtgcgcg ccatcgaccc gcgcccgggt caggccatgg tcgagatcgg ccccggcctg
    20221 gcggcgctga cgcagccgct ggtcgagcgc ctgggacgcc tgacggtgat cgagctggac
    20281 cgtgatctgg cggcgcggct gcgcgctcac ccgcagctcg aggtcatcga gtctgatgta
    20341 ctgaaagtcg atttcggtca gatacaacaa gcgcgagcag ctacaaaaat cagagtgtgt
    20401 ggcaacctgc cctacaacat ctccagcccg atcctgtttc acctgctgcc ctttgccgcg
    20461 ctgatcgagg accagcattt catgctgcaa aaggaagtga tcgaccgcat ggtggccgcg
    20521 cccgccacgg ccgactacgg ccgcttgtcg gtgatactgc agtggcgcta cgcgatggag
    20581 aacgtgctgt tcgtgccgcc cagcgcgttc gatccgccgc ccaaggtgga cagcgcggtg
    20641 gtgcgcatgg tgccgcgcag cgagtccgcc cccgtcgatg ccgacctact ctccgagttg
    20701 gtccgcgtcg ccttcagcca acgccgcaaa ctgctgcgcc acacgctggg ccgctggctg
    20761 agcgagcgcg gctttgccgg cgcgttcgac gtgcagcggc gcgccgagga agtgccggtg
    20821 gccgaatacg tggcgctggc gcatgaactc gctgcgcccc gatgacttcg cggcgttcaa
    20881 ggccatcgcg tcagttcagc gccatgctca gcttgcgccg gtagctggac agcagctcgg
    20941 cctgcgggtc ctgctgctgc gcggccttgc cggtgagctc gatgccgccg gcgctcttgc
    21001 cgccatcgcc ggcgccgggc ttggcgggcg gcggggtcag cagctccagg atggcgacgt
    21061 aggtcttgcg cgcggcctcg tcgttccact tcctgtcgcg caggatgatt tccagcagct
    21121 cgtccatgct ggccgtccac tggccctcgg ccaacagcgc ctgggccttg gcaaagcggg
    21181 tgtcgaagtc gcgcttgttc tgggcgatcc gggcgtcgaa ctgctcgagc ggccaattgc
    21241 cgcgctcgtc gcgctgcacg aactcgatcg cgtccagcca gcggcgcagg gcgtcgaagc
    21301 gcagcgggcg cggctcgcgc gccagcggct cttccagcgt ggcggcggct tcggacaggg
    21361 cgccggtggc gatcagcagg cgcgcgtagt cggcgcgggc gtcgtcgttg gccgggtcct
    21421 gcgccagcgc gtcggccagc ttggccagcg cggcctgggt gtcgccggcc tccagcaggg
    21481 cgccggcttc gtccagctcg gcctcggccg ccagcgcgcc ctcgctgggc acatgcttgt
    21541 ccagaaactg gcgcagctgc ccggccggca gcgcgcccat gaagccgtcg accggctggc
    21601 cgttcttcag caggatgcag gtcgggatgg agcggatgcc gaaggcgccg gccagctcct
    21661 gctgctggtc ggagtcgatc ttgaccagct tgaagcggcc ggcgtattcg acctccagct
    21721 gctcgaggat cggccccagc gtcttgcacg ggccgcacca gggcgcccag aagtccacca
    21781 gcaccggggt gtgcatcgag gcggcaatga cctcggcttc gaagttttga acggtgacgt
    21841 cgatcatgaa gggtatctgc gggcaaaatc ggggattacc agccattgtg caatagcgtc
    21901 atgacgactc ccctcgtggg cgtggtgatg ggttccagca gcgactggga caccatgcag
    21961 cacgccgtcc agatccttga gcagttcggt gtgccgcacg aagcgcgcgt ggtgtcggcg
    22021 catcgcatgc ccgacgacat gttcgcctac gccgaagccg cgccgcagcg cggcctgcgc
    22081 gccatcatcg ccggcgccgg cggcgccgcc catctgcccg gcatgctggc ggccaagacg
    22141 gtggtgccgg tgctgggcgt gccggtggcc tcgcgccacc tgcagggcgt cgattcgctg
    22201 cattccatcg tacagatgcc caagggcgtg ccggtggcca cctttgccat cggcgcggcc
    22261 ggcgccgcca atgcggcgct gttcgccgtc gccctgctgg ccaccaccga tgcggcactg
    22321 gcgcaaaggc tgcaggcctt ccgcgccgag cagaccgccg ccgcccgcgc catgaccctg
    22381 ccgccggcct gagtcgcagc atgaaccacg aacaccggta cgcagagaac gccacgatga
    22441 cgcacaggac gccagagaat ccaggttcga atctctgaat cttttgcgcc ctctgcgccc
    22501 ccttggcgcc ttcggcgtac ggatgttggg ttccttcaac ttgtcccgca ccatgtccat
    22561 cccacccccc ctgctgcctg gcaccctgat cgacggccgg cccgccaccc tgggtgtgct
    22621 gggcggcggg caactggggc gcatgttcgt gcacgccgcc caggccatgg gctatgagac
    22681 ggcggtgctc gaccccgacc cgcaaagccc cgccgggcgc gtgagccagc atttcatcca
    22741 ggccgactac cgcgacggcg ccggcctggc cgagctggcc cggcgggccc acgccatcag
    22801 caccgaattc gagaacgtgc cggccgacag cctggcgcag ctggccggca cgcggccggt
    22861 ggcgccggcc gccgccgccg tcgccatcgc gcaggaccgc agcgccgaaa aggcgcattt
    22921 cgcccgctgc gccggcgccg gcggtgtggc gccagcggcc cacgcgacca tcaccagcga
    22981 ggccgagctg gccgccgtgc cggccaccct gctgcccggc atcctgaaga ccgcgcgcct
    23041 gggctacgac ggcaagggcc aggcgcgcgt ggccacgcgc gccgagctgg ccgccgcctg
    23101 gcaggccatg ggccgcgcgc cctgcgtgct ggagcagcgc ctggcgctgg cgctggaatg
    23161 ctcggtgatc gtggcgcgcg ggcgcgacgg ccaggccgtc agcctgccgg tgcagcgcaa
    23221 cctgcaccgc gacggcatcc tggccgtcac cgaggtccat gagggaaatc tgcctcctgc
    23281 gcttgccagt caagcgcaag cagctaccat taagatagca aaccagctcg actatgtggg
    23341 cgtgctgtgc gttgagtact tcgtgctgga gggcggtgcc ctggtggtca acgaaatggc
    23401 gccgcgcccg cacaacagcg gccactacag cctggacgcc tgcgacgtct cgcagttcga
    23461 gctgcaggtg cgcaccctgg ccggcctgcc gctgcgcgcg ccgcgccagc acagcccggc
    23521 catcatgctg aacctgctgg gcgatctgtg gttcgacgcc gccggccgcc cgcgcacccc
    23581 gccctgggac gccctgctgc aactgcccgg cgcccacctg cacctgtacg gcaagaccga
    23641 ggcgcggcgc ggccgcaaga tgggccacct gacgttcacc ggcgccagca ccgaggcggt
    23701 gcgcgccagc gcccggcagg ccgccgccct gctgggcatg gaagccatcg gatgattctg
    23761 gacggcgccc gtcccgaggc catcgcccag gcggtggacg cgctggccgc cggccggctg
    23821 gtgggcctgc ccaccgaaac cgtctacggc ctggccgccg acgccggcaa cgccgccgcc
    23881 gtgcgcgcca tcttcgccac caagggccgg ccggccgacc acccgctgat cgtgcacgtg
    23941 gccggcgccg ccgccgtgcc ggccttcgcc agcgccgtgc ccgacttcgc ccagcgcctg
    24001 atcgacgcct gctggcccgg cccgctgacc ctgatcctgc cgcgccgccc cggcgtggcc
    24061 gacgccgccg ccggcggcca ccccagcatc ggcctgcgct gcccggcgca cccggtggcg
    24121 cgcgccctgc tggcggcctg cgcggcgcgc ggcatcgccg gcctggcggc gcccagcgcc
    24181 aaccgcttcg gccgcgtcag cccgaccacg gcggcccatg tggcggccga gttcggcgac
    24241 gagctgctgg tgctcgacgg cggtgcctgc aacgtgggca tcgaatcgac catcatcgac
    24301 gccacccgcg gcgcgccggt gctgctgcgc ccggggcagc tggcgcgcgc cgacatcgag
    24361 cgcctggcgg gtcggcaatt gctatccaaa gaagagctga tggcgcccga cccgcaagcg
    24421 ccaggcacgc tttcggccca ttacgcaccg acggccaggc tgcgcctgat ggacgcccgc
    24481 gagctgcgcg ccgcgctgca ggtgctgggc gccgaggcgc gccacatcgc ggtgtgggcg
    24541 cgcgcgccgc tgacctccgc ctcgcgccag ctgctgctgc gccgcatgcc cgagcgcgcc
    24601 gacgccgccg cgcgcgagct gtttgccgtg ctgcgcgagt tcgacgccgc tggcgcccgc
    24661 ctgatctggg tcgaaacccc gcccgacgac ccggcctggg acggcgtgcg cgaccgcctg
    24721 cagcgcgccg ccgcctcggc ctgagcctat ggggtcggaa tcctggaatt ggagaggccc
    24781 tcgtgccgca tgcgtgggtg agcgcgtgtg gccgaagcgc acgccggcgc gccgggctcg
    24841 gtcacacccg ctgcgggcgg cgccgctaaa ctacgccacc ctgttccaag caacacaaga
    24901 ctcatcatga acgcatcctg gtttcgccgc gccgccctgg cgctcggctg cgccggcacc
    24961 ctggccctgc tgacggcctg cggctccggc tcggtggtgt cggacctggc gcccaagcgc
    25021 tttctcaccg tgggcgacag cttcatggac gtcggccaga gcggcaagcg ctacaccgtc
    25081 aacgacggct ccaacaactg ggtgcaggag ctggcggccc attacggcgt caccgtcagc
    25141 gccgccagcg ccggcggcag cggccatgcg cagggccatg cgcgcatcgc cgcggccgac
    25201 ccgatcggcg gcgcgcccag cgtcaccacc cagatcgaca ccctgctggc cagcaccacg
    25261 ctgtccgaca ccgacgacgt ggtgctgatc aacggcggca tgcacgacat cgtggcggcg
    25321 gtcgaggccg gcggcctctc cgacgccacc acccaggccg tcaaggacgc cggcaaggcg
    25381 ctggccaccc aggtgcgccg cgtggtcagc gccggcggca agcatgtggt ggtgaccggc
    25441 gtgccgttgc tgggcgacag cccctgggcg cgcgcgcgca accagcgcga cggcatcaac
    25501 gcgctgtcgg tggccttcaa cgacgcgctg ctgtcgagca tcgtcgatct gggcgcccac
    25561 gtgctgtatt tcgacgccgc cttcttcttc aacctgatgt acggcaagcc gcagaacttc
    25621 gatctcgcca acgggctgga cccggtctgc accacgcccg acgccagcac ctgcaccgcc
    25681 gccaccgtgg cctcggccga ctacaaccgc tggctgttcg ccgacggcct gcatttctcg
    25741 cccggcgcgc agcgcaagtt cgcctccaac gactacgccg aaagcgccta ccgtcgcttc
    25801 aaggagcgct ggtaagcggc caccgccacc gcagcgcgca gatcagccgg ccacgcgccg
    25861 gctttttcgt ggccggcgct atgctcgcgc catgcaggag attcgcccat gaccccccca
    25921 cgcacccacc gcatcgccgt cattgcgggc gacggcatcg gccaggaagt gatgcccgag
    25981 gcgctgcgcg ccatcgacgc cgtccaacgc cgcttcggct ttgcgctgca gcagaccccg
    26041 atcgcctggg ccagctgcga ctactacgcc cgcaccggcc agatgatgcc ggacgactgg
    26101 aaggcgcagt tgcaaggcat ggacgccctg ctgttcggcg ccgtcggctg gccggccacc
    26161 gtgcccgacc atgtgtcgct gtggggcagc ctgctgaaat tccggcgcga gttcgaccag
    26221 tacatcaacc tgcgcccggt gcgcctgttc gacggcgtgc cctgcccgtt ggccggtaaa
    26281 aagcccggcg acatcgactt cctcgtcgtg cgcgaaaaca ccgagggcga gtacaccaac
    26341 ctgggtggcg tgatgtatgc cggcaccgag cgcgaaatcg tggtgcagga aacggtgatg
    26401 agcgccttcg gcgccgaccg cgtgctcaag ttcgccttcg agcaggccgc cgcccgtccg
    26461 cgccaccagc tggacgtggc gaccaagagc aacggcatcg ccatcagcat gccctggtgg
    26521 gacggccgcg ccgacgccat ggcggcgcgc tacccgcagg tggccgtgca aaagcaccac
    26581 atcgacatcc tcacggcgcg cttcgtgctg cagcccgagc gcttcgacgt ggtggtggcc
    26641 agcaacctgt tcggcgacat cctgagcgac ctgggcccgg cctgcaccgg caccatcggg
    26701 ctggcgcctt cggccaacct caaccccgag cggcgctttc ccagcctgtt cgagccggtg
    26761 cacggctcgg cgccggacat cttcgggcgc ggcatcgcca acccggtggc catgatctgg
    26821 tcggccgcgc tgatgctgca gtttctgggc gagcaggcgg cgcacgacgc catcctgcgc
    26881 gccatcgaac aggtgctggc ggcaggcccg cgcacgcccg atctgggtgg cacggccagc
    26941 accgtacaga tggggcaggc catcgcggcg cgcatcgagg ccggcgcctg agccggtctg
    27001 cggcacttca cgattcggct gcccgcgctt ctgcggggcg ctcggttgct atataacata
    27061 tagctttcgg cgcttgcctg aagggcgcgg cagccctgaa cctaggctga acctagaaac
    27121 ggcagttgac cgctggtcaa cttcgggcgg accgcacccg tgcagggttt cgcgactgcg
    27181 atggccttcg cctcctgggt gagcacgctg cgcacccccg caccgcgctc ggcgcgccat
    27241 gcggcgttgc tcctgcctgc gggcaccagc ccgcggcggc gtcgcgtctt gcctggcacg
    27301 ccgatcacgg ca
//
LOCUS       DAOQTZ010000027        26755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2114_length_26755_cov_14.352060, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000027 DAOQTZ010000000
VERSION     DAOQTZ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 26755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26755
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2114_length_26755_cov_14.352060"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKN51_04970"
     CDS             complement(<1..453)
                     /locus_tag="PKN51_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84483.1"
                     /translation="MLIDFFYALRAAKLPVSVKEFLTLLEALQANVVGPRQPDACTMD
                     DFYYLARTALVKDEKHFDKFDRAFAAYFKGVEMLTDFSKEIPADWLRKMLERELTPEQ
                     KAAIEKMGWDELMETLRKRLEEQKGRHEGGNKWIGTGGTSPFGHGGYNP"
     gene            complement(472..1074)
                     /locus_tag="PKN51_04975"
     CDS             complement(472..1074)
                     /locus_tag="PKN51_04975"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNT84484.1"
                     /translation="MAFVEPVTLRGHGLRLEPLALTHEAGLAAAAADGALWRIRVTSV
                     PEPQETRAYIETALQMREAGSRLAFAVLDDASGRMLGSSSYHDILPAVRRVEIGYTWY
                     ARSVQRTHVNTAAKLLLMGHAFDTLGCHVVGWRTDNFNHASQRAIERLGARKDGVIRG
                     HALRRDGTIRDTVMYSMRSGEWPEARAQLLYLLELRAQAR"
     gene            complement(1078..1302)
                     /locus_tag="PKN51_04980"
     CDS             complement(1078..1302)
                     /locus_tag="PKN51_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84485.1"
                     /translation="MHPLLVEGLSDAVGFVGGALAGFWLARLLGFDVFSEGYDSASVL
                     AIAAVGLGGGLGLGAARRWRRARQAERKER"
     gene            complement(1322..2164)
                     /locus_tag="PKN51_04985"
     CDS             complement(1322..2164)
                     /locus_tag="PKN51_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT84486.1"
                     /translation="MKFQGTDSYVATQDLMLAVNASITLKRPLLVKGEPGTGKTMLAE
                     EVAQALGLPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDDRVKDIHNYIVKGVLWQ
                     AFTADEPVALLIDEIDKADIEFPNDLLRELDRMEFHCYETRETIRARHRPVVFITSNN
                     EKELPDAFLRRCFFHYIKFPDADTMRQIVDVHFPDLKKELLAAAMKVFFDVRGLPGLK
                     KKPSTSELLDWLKLLVAEDIPAEALQSQDDKVAVPPLVGALLKNEQDVTLFEKLVFMN
                     QRHR"
     gene            2294..2938
                     /locus_tag="PKN51_04990"
     CDS             2294..2938
                     /locus_tag="PKN51_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNT84487.1"
                     /translation="MFAALGTALAMTLPVQAQVQGNATAGEGKVAMCIGCHGIAGYKS
                     SFPEVYRVPKIAGQSAKYIGTVLEEYRKGTRKHPSMRGIAGSMSDQDIADVAAYYEAL
                     GKVEGATELPAPREPSAQVAQLLQKGACVSCHGDNYSKPIDPSYPKIAGQYPDYLYAA
                     LRAYRDDSHATWGRGNPVMAGMAKQFSDPELKAMARYIGSLPGEVRTVPQSRFR"
     gene            complement(3228..3668)
                     /locus_tag="PKN51_04995"
     CDS             complement(3228..3668)
                     /locus_tag="PKN51_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HNT84488.1"
                     /translation="MFAPSQADVRRFFCHAWAKARGAQPMDAIEQLAAGWIAEHPEWH
                     ADFADAEAAVARHYGDGAVGGNPFLHLSMHLSISEQCSIDQPRGIRQAVELLAHRRGD
                     LHAAHHEVMECLGQMLADAQSRRAAPDGDGYVACVQRRATRDGA"
     gene            complement(3689..4414)
                     /locus_tag="PKN51_05000"
     CDS             complement(3689..4414)
                     /locus_tag="PKN51_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84489.1"
                     /translation="MSETTDTVSERQACTHALTHWLTEARHQRQRAAAEPRRAARRQA
                     LRAFQAARLARTHADLLDSPRYGRAAAFFLTDLYSPKDLGPRDAEIERVLPLITSTLP
                     TAGLHALLQAAELDALSERFDAAMVHALGERLDTSLDDADYAAAYRTVDDAPGRQHQI
                     ALILGTGQTLDRLAHKPGLAALLKMMRGPAQLAGLGELQSFLERGFTAFRSMRSAQAF
                     LDTIIERERALSARLFGGGQSLA"
     gene            4605..5480
                     /locus_tag="PKN51_05005"
     CDS             4605..5480
                     /locus_tag="PKN51_05005"
                     /inference="COORDINATES: protein motif:HMM:NF018525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNT84490.1"
                     /translation="MLSALLLWGMAVELLVWGAIGRAGLGWSWGATLALALGALLLGR
                     ALLLAVTWLVAWRHRSAAPPLSAGAWLVMLWREYWAFVGGFVLFQPWARLAMPADRLR
                     PSAQPILLVHGYGCNRAIWWWLRPRLEAAGHVVATLSLEPPWGDIDGFAEQLQQRIEA
                     VCAATGAPRLTLVAHSMGGLVSRACLARHGAARVAALISIASPHGGSRLAHLGLGRCA
                     RQMQPGSHWLARLAQQRVNVPFVSIRTPQDNFVMPQQLQRHADARDEPLPGVGHMAAL
                     CDERTLRLVLAHTKL"
     gene            complement(5564..7543)
                     /locus_tag="PKN51_05010"
     CDS             complement(5564..7543)
                     /locus_tag="PKN51_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT84491.1"
                     /translation="MQYFITGATGFIGKRLVARLLEREGARIFFLVRQRDPERLQALY
                     DFWGCDDTRATPVVGDLTQDMLGVSAADRRKLGKKTTHFFHVAAVYDLSADAERQLQV
                     NVEGTRQAVRFAEAIGAKHFHLFSSIAAAGLYEGVFREDMFEQAEGLDHPYFRTKHDS
                     EGIVRNECRIPWRVYRPAIVVGDSRTGEMDKIDGPYYFFKLIQKLRRLLPAWMPAIGI
                     EGGRINLVPVDYVVDAVDHIAHLKGEDGRCFHLVDPRPRRVGDVLNIFARAGHAPQMS
                     VRVNAALLGFIPKYMRKALLALTPVRRVRDVLMKELGLPDDILSFVNYPTRFDSRETE
                     RVLKGTRIAVPPLEDYAWRLWDYWERCLDPDLFIDRSLGGRVKGQVVVVTGASSGIGK
                     AVALRLAEAGAIVVTMARGKPALDAAASEFEALGLKLHTYEADLADLAQVDAVAQQIL
                     HDHGPVAILINNAGRSIRRAIENSYERFHDFERTMQLNYFGALRLNLNLLPAMAERRT
                     GHIINISSIGVLTNAPRFSAYVASKAALDAWTRCAASEFADRGIHFTTINMPLVKTPM
                     IAPTALYDQAPTLTPEEAADMVCEAIVHRPTRMATRLGITGEVLHALMPRVAQAVMNT
                     SFRMFGESQAAASGEKTAPTRDQLALQNWMRGIHF"
     gene            7726..8202
                     /locus_tag="PKN51_05015"
     CDS             7726..8202
                     /locus_tag="PKN51_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNT84492.1"
                     /translation="MASKLKTISDNNQLAATIKESTQQIWLAGLGAFAKAQEEGGKMF
                     DALVKEGEMMQKKTREVADARIAAVAGRAADTWDRLEQVFEDRVARALGSLGVPSKKE
                     VDVLSKRIAELTAAVQELAQNKGKAAPRLPAAKPAVAKPAPAKKAAPAKKAARKSA"
     gene            8372..9547
                     /locus_tag="PKN51_05020"
     CDS             8372..9547
                     /locus_tag="PKN51_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNT84493.1"
                     /translation="MSKIGLALAGGGPLGGIYEVGACVALEEAIDGLSLVDADVYVGV
                     SAGSFVAASLANGFEPRRLARMLLGSDGSQAFDPSLLLRPALREYLNRARAIPRLALQ
                     AAREYLGNPWRHGLFESFQRLSRAIPTGLFDGAGVDAVLRRQFSEVGRSNDFRRLKAR
                     LYVVATDLDSGQAVAFGGPGRDDVPISTAVQASAALPGLFPPVEIQGRHYVDGALIKT
                     LHASVALRDGVDLLLCVNPLVPFDAQLAEQHGAGHRQLSEGGLPVVLAQTFRAIIRSR
                     MRVGMDRYQHQFKHADVLLFEPAQDDEAMFFANLFSYRDRARLCEHAYQRTREHLYQR
                     RDELAPVLARHGLQLNLEVLRDRTRTLLGAEPAATATQGLARTLDQLQHWIDARRAA"
     gene            9600..10256
                     /locus_tag="PKN51_05025"
     CDS             9600..10256
                     /locus_tag="PKN51_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT84494.1"
                     /translation="MNKPKRRTPERILETALRLFNEFGEPNVTTTVIADEMNISPGNL
                     YYHYRNKDEIVNALFARYEREISELLTLRDTAPREVEDVWFFLHLLFETISTYRFIYR
                     DINDLLTRNRLVETHFQRILAHKQQTAVALCHGLAAAGALDATPDEIAALAVNITLVT
                     TFWLSFEHARHPRRPPDIGRGAHQVLSLTAPWLREPARRLLEKLSLSYVHSTSRGDAR
                     "
     gene            10253..11038
                     /locus_tag="PKN51_05030"
     CDS             10253..11038
                     /locus_tag="PKN51_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84495.1"
                     /translation="MSKTAWKAFPHPDPAYTYAGTALKKHWARLHQGDAEPWPTDSAA
                     QAAWRAYHAGEFAQAVALGLKAGNASGTNAANKAAMIHAASVEDDQARKLALLQEVAR
                     RCEALQTSQPGNVNAWYYHGYALGRYSQGISVAKALAEGLGSKVRESLQKAIDLEPRH
                     ADAHIALGTWHAEIINKVGAVVGGLTYGAKKDAAEKHFKTALALNPESPIAMTEYANG
                     LAMMFGKSRLKEAEQLYARAAQCTPADAMERLDVEVARAEVAG"
     gene            complement(11134..12588)
                     /locus_tag="PKN51_05035"
     CDS             complement(11134..12588)
                     /locus_tag="PKN51_05035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNT84496.1"
                     /translation="MTAQEKMSPVDTAWLRMDRPNNLMQILGVMLFDGKLDFARLRQR
                     IEQRLLSHARFRQFAREDATGWSWVDDPEFDLDNHLRRAALPGKGDKAELERFVADLA
                     STPLPPARPLWQIHVVDTHMGGQALVTRFHHCIADGIALVAVIMEMADDAPAPLPAAA
                     ATPPHHSHDDALWGMVWHPISRAVQASVRASTSLWAMSQDWLRNPWQALDYARLGSGM
                     TAEAAKLLVMPNDSTTRFKGTPGLVKRVAWSEPIDLREIKAVGKVLGCSVNDLLLSAV
                     AGALGAYLTDCGDATEGVELRALVPVNLRQAADAGKLGNRFGMVTLELPVGVANPLAR
                     LYETRRRMQAMKQSYQPALTLTLLGAAGMGPKLVQEQLLDFLANKATAVMTNVPGAQQ
                     PLYFAGARLRQPFFWVPQSGNIGMGVSILSYANQVQFGLITDRRLVPDPERIVSRFAE
                     EFDKLLMLVLLEPPECLHDPELVEAHNAFYWSAE"
     gene            complement(12617..12907)
                     /locus_tag="PKN51_05040"
     CDS             complement(12617..12907)
                     /locus_tag="PKN51_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HNT84497.1"
                     /translation="MSQISITRAHGLSPKKARLAAEAVAADLQAEYGLQYAWDDDGTL
                     SFQRAGISGQLTLERRQVTVQVRLGLLFLPFKASFEREIHDYFDQRFAPRSA"
     gene            12986..13702
                     /locus_tag="PKN51_05045"
     CDS             12986..13702
                     /locus_tag="PKN51_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HNT84498.1"
                     /translation="MIDKIAASVAEALADVRDGATVLIGGFGTAGIPGELIDGLIAQG
                     ARELTVVNNNAGNGDRGLAALLAAGRVRKIICSFPRQADSQVFDGLYRAGRIELELVP
                     QGNLAERLRAAGAGIGAFFTPTGFGTELAKNPDGSPKETRRINGRDHVLEYPIHGDVA
                     LIRAERGDRWGNLSYRMAARNFGPVMAMAARCTVASVHEVVALGQLDPEAIVTPGIFV
                     HRVVRVPRVATAAGGLRSAA"
     gene            13730..14374
                     /locus_tag="PKN51_05050"
     CDS             13730..14374
                     /locus_tag="PKN51_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HNT84499.1"
                     /translation="MSSYQKRSKDELARRVAQDIPDGAYVNLGIGQPTAVANHIPPGR
                     EIILHSENGILGMGPAPAAGQEDYDLINAGKQPVTLRPGGAFFHHADSFAMMRGGHLD
                     VCVLGAFQVSVTGDLANWSTGEAGAIPAVGGAMDLAVGARQTWVMMDLLTRAGESKIV
                     ARCSYPLTAIGCVRRIYTDLATFACSPQGLRLIDRVEGLSLDELRRLVGQPFAA"
     gene            14430..15632
                     /gene="pcaF"
                     /locus_tag="PKN51_05055"
     CDS             14430..15632
                     /gene="pcaF"
                     /locus_tag="PKN51_05055"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HNT84500.1"
                     /translation="MTQAFIVDAIRTPFGRYGGALSGVRTDDLGAIPIRALMARHPRV
                     DWQAVGDVLYGCANQAGEDNRNVARMSGLLAGLPIEVPGATINRLCGSGLDAVGTAAR
                     AIRAGEARLMIAGGVESMSRAPFVMPKAESAFSRANAVYDTTIGWRFVNRLMKAQYGV
                     DSMPETAENVAVDYRIEREAQDRMALASQMKAVAAQQAGHLAREITPVSIAQKKGDPI
                     VVEHDEHPRATSLDKLAALKGVVRPDGTVTAGNASGVNDGACALLLADEATASQYGLT
                     PRARVVGMAVAGVAPRVMGIGPAPATEKVLQLTGLKLAQIDVIELNEAFAAQGLAVLR
                     LLGLADDDERVNAWGGAIALGHPLGASGARLATTAINRLHTTGGRYALCTMCIGVGQG
                     IALVLERV"
     gene            complement(15803..16453)
                     /locus_tag="PKN51_05060"
     CDS             complement(15803..16453)
                     /locus_tag="PKN51_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNT84501.1"
                     /translation="MNLLAPLPPGWSELLRTALEVAATLAFALSGVIAAARQRLDVVG
                     VCVVAFAAAFGGGTLRDLLLDQRPFFWVRHIEFVWALLALCLAAMALMRQRELALTER
                     AILWPDAIGLGLFTAIGVDLGLAVGLPALAAVMMGVITSCFGGVLRDVLCNLLPSAFS
                     DHRPYALWSFAGGWLHVGLLAAGLPPWLVLTVTVLFTAGMRLVSVWRGWSLPGWRV"
     gene            16562..17314
                     /locus_tag="PKN51_05065"
     CDS             16562..17314
                     /locus_tag="PKN51_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT84502.1"
                     /translation="MTPITDWGFYAVAVPAVLLLGVSKSGFGAGFGSLAVPLMALSVS
                     VPQAAAILMPVLLVMDLLGIAAFRKDFDRALLRFMLPWGLLGIVVGTLSFRLLQAHWV
                     AGIVGGFTLLFLAQRLLFAPRADAAPPPRWAGALLTATSGFTSFVAHAGGPPVNAYVI
                     PLGLAPLRFTGTMAFFFFVINLAKWLPYAWLGLLDGRNLGTSLVLLPLAPIGVWIGVR
                     IARRIDQRLFYRFVYLGMLLTGLKLLWDAVAA"
     gene            complement(17328..17843)
                     /gene="lspA"
                     /locus_tag="PKN51_05070"
     CDS             complement(17328..17843)
                     /gene="lspA"
                     /locus_tag="PKN51_05070"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNT84503.1"
                     /translation="MARAASRGGSLAVWLTLAAVVVVLDQFTKQLILGHYRLGDSTTV
                     TSFFNVVRAHNTGAAFSFLADAGGWQRWFFVALALAVAGFIVWLLRAHPGQKLFAFSL
                     SMILGGAVGNVIDRLLHGYVVDFLDFHWPFLAPLFHHGHFPAFNLADAAITAGAIGLM
                     LDELLRVRRGR"
     gene            complement(17846..20680)
                     /gene="ileS"
                     /locus_tag="PKN51_05075"
     CDS             complement(17846..20680)
                     /gene="ileS"
                     /locus_tag="PKN51_05075"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNT84504.1"
                     /translation="MSTDAKTDYRQTLNLPDTPFPMRGDLPKREPGWVQAWEDQGLYR
                     RLRAARAGRAKFILHDGPPYANGQIHIGHAVNKVLKDMIVKARQLKGMDAHYVPGWDC
                     HGLPIENQIEKQFGRHLSRDEMQAKGRAYATEQIASQMKDFRRLGVLGEWDKPYKTMD
                     FANEAGELRLFKRLIERGFVYRGLKPVYWCFDCGSALAEAEIEYQDRRSTTVDVAFKA
                     HDPAALAAAFGLPHLGKPAYAVIWTTTAWTIPANQALNLGPHIEYALVDTARGLLLLA
                     KARVADCLQRYGLQGEIIATAMGDKLGGLAFEHPLYEVDAAYRRLAPVYLADYATDSD
                     GTGSVHSAPAYGVEDFNSCVAHGLAHADILNPVQGGGSYAPDFALFGGLDIWKAQPAI
                     IEALDQAGRLLASGKLEHSYPHCWRHKSPVIYRAASQWFVRMDEGEGVFTTDKAPETL
                     RTTALRAIEQTAFYPPSGQARLRDMIAGRPDWVISRQRAWGVPIPFFLHKETDALHPR
                     TMEIIDQAAAIVEQGGIEAWSRVTAEEILGPEDAQHYTKSSDILEVWFDSGSTFWHVL
                     CGTHPAEHHEHGPEADMYLEGHDQHRGWFHSSLLLASAIRGRAPYRSLLTHGFTVDGQ
                     GRKMSKSLGNAVAPQVTSEKLGAEIIRLWVAASDYSGDIGIDEKILARVVDSYRRIRN
                     TLRFLLANTSDFDPHKHAVPAAQLLELDRWLLARAAEFQAELLAHYEVYEFHPVVAKL
                     QLFCSEDLGGFYLDVLKDRLYTTAPDSPARRSAQTALWQLTQAMLRWMAPFLSFTAEE
                     AWQLVGTSDSIFLEEYWSFDAPDAALLAKWARIRAIREQVNKEIEVQREQGAVGSSLQ
                     ANVVLGAPPDDHALLASLGADLKFVCIVSAIALEAAGALSVSVSASKDTKCERCWHYL
                     PDVGRHAEHPTLCGRCVSNLFGAGETRTVA"
     gene            complement(20803..21816)
                     /locus_tag="PKN51_05080"
     CDS             complement(20803..21816)
                     /locus_tag="PKN51_05080"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNT84505.1"
                     /translation="MKIFRGLRHPGMAPGCALTIGNFDGVHRGHQAMLALLRNEAAHR
                     GVPSCVMTFEPHPRDHFAAVQQRPELAPARIATFRDKLAELARCGIDQAVVLPFNQAL
                     ASQPPQDFIDQVLVQGLGVRYVLVGDDFRFGARRAGDYAMLDAAGQRLGFDVARMLSY
                     EVHGQRVSSSAVREALAAGDMAAAAALLGRPYAISGHVVHGRKLGRALAEAAPGRGDG
                     FRTLNLRFSRRAHAWKPAASGIFVVEVHGLDARPLPGVANLGVRPSLDPHDVNGGRVL
                     LETHVLDWPARLGPEGAYGKIIRVDLLHKLHDELRYHSLAALTEGIARDCAAARDWLA
                     ARI"
     gene            21995..22552
                     /locus_tag="PKN51_05085"
     CDS             21995..22552
                     /locus_tag="PKN51_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNT84506.1"
                     /translation="MKVLKLSAQGLPQSWISLEQAVIHYAAEEVRWEAGGQIAVFRGG
                     HNAITGEQSIVRINSIIGTHGAPRINPFELRPSLTNAKLFARDRNLCAYCGQHYTEAE
                     LTREHVVPQAQRGRDHWMNVVTACRACNHRKSNRTPEQAHMPLLYTPYVPSLWEDFIL
                     RNRRILADQMEFLMAHLPRSSRLHA"
     gene            22587..23183
                     /locus_tag="PKN51_05090"
     CDS             22587..23183
                     /locus_tag="PKN51_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT84507.1"
                     /translation="MPRLIFFCGHAGTGKTTLARRAIAALMRQAHARCSGESFCLLDK
                     DTLYGQYSSAVMGVLTGDPNDRDSPPYLQHLRQPEYDGLLATARENLALGVSCIVVGP
                     LSREVRAHRLTDRAWLAVGDDVRVHVVWVHLEEAEARRRITARGNPNDAWKLAHWDAY
                     RTRLFVPTAAEYPELLQYDNTDAPPAAFEGLLEALADR"
     gene            complement(23575..25011)
                     /gene="mdtD"
                     /locus_tag="PKN51_05095"
     CDS             complement(23575..25011)
                     /gene="mdtD"
                     /locus_tag="PKN51_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HNT84508.1"
                     /translation="MSPVLPPPSPAGGSPRLLLWLVAIGFFMQTLDSTIVNTALPAMA
                     RSLGESPLRMQAVVVSYALTMAAVIPATGWLADRFGTRRVFALAIALFTLGSAACAAA
                     PSLTLLTAARVLQGVGGAMLLPVGRLAVLQAFPRERFLEAMSFVAIPGLVGPLIGPTL
                     GGWLVEVASWHWIFLINLPVGLLGLLATRRHMPNLRAAQAGRFDLGGYLLLVLAMVSI
                     SLSLDGLGGLGWARTLVVLLMVLGLAALAAYWLHAQRSPAPLFAPALFGVASYRVGVL
                     GNLFARIGSGAMPYLLPLLLQLALGFSPAQAGMMMLPVALASMGIKRPITRVIHRLGY
                     RPVLMGNTVLLGLMIAGFALMTPQQPLWLRIVQLALFGAANSTQFTAMNAVTLKDLAP
                     QRAASGNSLFSMVQMLGMSLGVTCAAALLHAFSDWLGGAGAAQSLPAFRATFVAIGLM
                     TMGSAAIFWQLRPDEPRQPPTGALVDVG"
     gene            complement(25080..26195)
                     /locus_tag="PKN51_05100"
     CDS             complement(25080..26195)
                     /locus_tag="PKN51_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT84509.1"
                     /translation="MDFDFSDDQLALRDAVAKWVEKSYDFERRRAIVAAGGFDRAAYG
                     ELAELGLAGICVGEDHGGLGMGPVEAMVVLEELGRGIVVEPLTQTFVVSTTLGAHGSD
                     AVRQAWLPRIASGEALVVLAHQERKARYRLDTCEAQASATGGGHTVTGLKHIVAAGDQ
                     ADAWLVPAQLNGKLALFLIERGAQGVNTRGYLTQDGSRAADVQFNGAPAQLVTTDGLT
                     ALMHAADVGIACACAYGVGAMDQTLKLTVDYMNQRKQFGVVIASFQALRHRVADMKMQ
                     LELARSMSYYATLKLGAPEAERRQALSRAKVQLGQALRFVGQQSVQLHGGIGVTDEYV
                     GSHYFKSLTQLEMSWGDTLHHLGQVSQRMTDTAGVFA"
     gene            complement(26219..>26755)
                     /locus_tag="PKN51_05105"
     CDS             complement(26219..>26755)
                     /locus_tag="PKN51_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT84510.1"
                     /translation="NLIGEENKGWTYAKHLLSHERTNIADVNRAKRELERLKRIARAE
                     GVWDDARFRDQIALLEVDIVALEMLVLRVLSAEKSGRNALDIAGLLKIKGSEIQQRYT
                     ELMMLAAGPFSLPYIWEAMEAGWQGDQAAAAGLQGFPGGAVANATLAASYFNYRKTTI
                     YGGSNEVQRNIVAQTVLG"
BASE COUNT         4061 a         9179 c         9489 g         4026 t
ORIGIN      
        1 cgggttgtag ccgccgtggc cgaaggggct ggtgccgccg gtgccgatcc acttgttgcc
       61 accttcgtgg cggcccttct gctcttccag gcgctttctc aaagtctcca tcaactcgtc
      121 ccagcccatt ttctcgatgg cggccttctg ctcgggcgtg agctcgcgct ccagcatctt
      181 gcgcagccag tcggccggga tctccttgct gaagtcggtc agcatctcga cgcccttgaa
      241 gtaggcggcg aaggcgcggt cgaacttgtc gaaatgcttc tcgtccttga ccagcgcggt
      301 gcgcgccagg tagtagaagt cgtccatcgt gcaggcgtcg ggctgccggg ggccgaccac
      361 gttggcctgc agcgcttcga gcagggtgag gaactccttg accgacaccg gcagcttggc
      421 ggcgcgcagg gcgtagaaga aatcgatcag catgaaaaag gcctccagcg cttaccgggc
      481 ttgcgcccgc agctccaata agtagagcaa ctgggcgcgc gcttcgggcc attcgccgct
      541 gcgcatgctg tacatcacgg tgtcgcggat ggtgccgtcg cggcgcagcg cgtggccgcg
      601 gatcacgccg tccttcctgg cgcccaggcg ttcgatggcg cgctggctgg cgtggttgaa
      661 gttgtcggtg cgccagccca ccacatggca gcccagggtg tcgaaggcgt ggcccatcag
      721 cagcagcttg gcggcggtgt tgacgtgcgt gcgctgcacg ctcctggcgt accaggtgta
      781 gccgatctcg acccggcgca cggccggcag gatgtcgtgg tagctgctgc tgccaagcat
      841 gcggccgctg gcgtcgtcga gcacggcaaa ggcaaggcgc gatccggctt cgcgcatctg
      901 cagcgcggtt tcgatgtagg cgcgggtttc ctggggctcg ggcaccgagg tgacgcggat
      961 gcgccacagc gccccgtcgg cggcggccgc ggccaggccg gcctcgtgcg tcagcgccag
     1021 cggctccagg cgcaggccgt ggccgcgcag cgtgacgggt tcgacgaagg ccatggctca
     1081 gcgctccttg cgctcggcct ggcgcgcgcg gcgccagcgg cgcgccgcac ccagccccag
     1141 gccaccgccc aggccgacgg cggcgatcgc cagcacgctg gcgctgtcgt agccttcgga
     1201 gaagacgtcg aaacccagca gccgcgccag ccagaagccg gccagcgcgc caccgacgaa
     1261 gccgaccgcg tcggacagac cctcgaccag cagcggatgc atggcgccgg ctcccggagg
     1321 ttcagcggtg acgctggttc atgaacacca gcttctcgaa cagggttacg tcctgctcgt
     1381 tcttgagcag cgcgcccacc agcggcggca cggcgacctt gtcgtcctgg ctttgcagcg
     1441 cctcggccgg gatgtcctcg gccaccagca gcttgagcca gtcgagcagc tcgctggtgg
     1501 agggcttttt cttcaggccc ggcagaccgc gcacgtcgaa gaacaccttc atggcggcgg
     1561 ccagcagttc cttcttgagg tcggggaagt gcacgtccac gatctgccgc atggtgtcgg
     1621 cgtcggggaa cttgatgtag tggaagaagc accggcgcag gaaggcgtcg ggcagctcct
     1681 tttcgttgtt ggaggtgatg aacaccaccg ggcgatgcct ggcgcggatg gtttcgcgcg
     1741 tctcgtagca gtggaactcc atgcggtcga gctcgcgcag caggtcgttg ggaaattcga
     1801 tgtcggcctt gtcgatctcg tcgatcagca gggccaccgg ctcgtcggcg gtgaaggcct
     1861 gccacagcac gcccttgacg atgtagttgt ggatgtcctt gacgcggtcg tcgcccagct
     1921 ggctgtcgcg caggcggctg acggcgtcgt actcgtacag gccctgctgc gccttggtgg
     1981 tgctcttgat gtgccactgc agcagcggca ggcccagcgc ctgcgccacc tcttcggcca
     2041 gcatggtctt gccggtgccg ggctcgccct tgaccagcag cgggcgcttg agcgtgatgg
     2101 aggcgttgac ggccagcatc aggtcttgcg tggcgacgta ggagtcggtg ccttggaatt
     2161 tcatgtcgga cagcggtgtg gatagcgggg aaacccaggg gtcgaaagcg caagcgtgat
     2221 gattacaatc cgcctctgat ctggatcaaa caacatggat tgtgcgcgca aaatgaaact
     2281 cagcctgtct ccgatgttcg ctgcactcgg caccgccctg gcgatgacgc tgccggtgca
     2341 ggcgcaggtc cagggcaacg ccaccgccgg tgagggcaag gtcgccatgt gcatcggctg
     2401 ccacggcatc gcgggctaca agtcgagctt ccccgaggtc tatcgcgtgc ccaagatcgc
     2461 cggccagagc gccaagtaca tcggcaccgt gctggaagaa taccgcaagg gcacgcgcaa
     2521 gcaccccagc atgcgcggca tcgccggctc catgagcgat caggacatcg ccgacgtggc
     2581 ggcctactac gaggcgctgg gcaaggtcga aggcgccacc gagctgcccg cgccgcgcga
     2641 accgagcgcg caggtggccc agctgctgca aaagggcgcc tgcgtgtcct gccatggcga
     2701 caactattcc aagccgatcg atcccagcta ccccaagatc gccggccagt accccgacta
     2761 cctgtacgcc gcgctgcgcg cctaccgcga cgacagccac gccacctggg gccgtggcaa
     2821 cccggtgatg gccggcatgg ccaagcagtt ctccgacccc gagctcaagg ccatggcgcg
     2881 ctacatcggc agcctgccgg gtgaagtgcg caccgtgccg cagtcgcgct ttcgctgagc
     2941 gcgccagcgc cgcccatcca gccaggcccg ccgcgtgcgg gccttttcat catttgtgcc
     3001 tccagcgcac gcccagcaag cgccacaagc tatcaaaaca ggagtgattg gatggggccc
     3061 gggcacgctc gttgaccgcc agcgctctgc gacctgcccg aagatgacaa gcggtcaacc
     3121 gccgtttcca ggtttcatca cccgcgggtc atgggtcatg ccggcgctgc gggtgtcgtt
     3181 ggtcgcatgg ttcgaacgcg aagccgcctg aagacggccg tccggggtca ggcgccgtcg
     3241 cgggttgcgc ggcgctgcac acaggccacg tagccgtcgc cgtccggggc ggcgcggcgg
     3301 ctctgcgcat cggccagcat ctgacccagg cattccatca cctcgtggtg cgcggcgtgc
     3361 aggtcgccgc ggcggtgcgc cagcagctcc accgcctgac ggatgccgcg cggctgatcg
     3421 atgctgcact gctcgctgat cgacaggtgc atcgacaggt gcagaaacgg gttgccgccc
     3481 accgcgccgt caccgtagtg gcgcgccacg gccgcttcgg cgtcggcgaa atcggcgtgc
     3541 cactcggggt gctcggcgat ccagccggcg gccagctgct cgatggcgtc catgggctgg
     3601 gcgccgcggg ccttggccca ggcgtggcag aagaagcggc ggacgtcggc ttggctgggg
     3661 gcaaacatgc ggcattgtcg ccgcaggctc acgccaggct ctggccaccg ccgaacagcc
     3721 gtgccgacag cgcgcgctcg cgctcgatga tggtgtccag aaacgcctgc gcgctgcgca
     3781 tgctgcgaaa ggcggtgaag ccgcgctcga gaaacgactg cagctcgccc agcccggcca
     3841 gttgcgccgg gccgcgcatc atcttcagca gcgccgccag gccgggcttg tgtgccagcc
     3901 ggtccagcgt ctggccggtg cccaggatca gcgcgatctg gtgctggcgg ccgggcgcat
     3961 cgtccacggt gcggtaggcg gcggcgtagt cggcgtcgtc gaggctggtg tccagccgct
     4021 cgcccagggc atggaccatg gcggcgtcga agcgttcgga cagcgcgtcc agctcggccg
     4081 cctgcagcag cgcgtgcagg cccgcggtcg gcagggtgct ggtgatgagc ggcagcacgc
     4141 gctcgatctc ggcgtcgcgc ggccccaggt ccttcgggct gtacaggtcg gtcagaaaga
     4201 aagccgcggc gcgcccgtag cgcgggctgt ccagcaggtc ggcgtgggtg cgcgccagcc
     4261 gtgcggcctg aaaggcgcgc agggcctgcc ggcgggcggc gcgccgtggc tcggcagcgg
     4321 cacgctggcg ctgatggcgc gcctcggtca accagtgggt cagcgcgtgg gtgcaggcct
     4381 gccgctcgct gaccgtgtcg gtggtctcgc tcatgcaggc attatccgaa gccgccgcgc
     4441 ccgggtttag aagcggtcgc ctagttgcag tgtttcgcgc cgtcaggtac ttacagcgag
     4501 tcgccaaggg gccagctgcg cggcgcgtgg ccgcaggcat ggtggatcaa tgtttcagtg
     4561 gactagactg aaaggctggt acatcaagaa gggagatcct gcgcatgctg tcggcgctgt
     4621 tgctgtgggg aatggcggtc gagctgctgg tctggggcgc catcgggcgc gcggggctgg
     4681 gctggtcgtg gggcgccacg ctggcgctgg cgctgggcgc gctgctgctg gggcgggcgc
     4741 tgctgctggc cgtcacctgg ctggtggcct ggcgccatcg ctcggcggcg ccgccgctgt
     4801 cggcgggcgc ctggctggtc atgctgtggc gcgaatactg ggccttcgtg ggcgggttcg
     4861 tgctgtttca gccctgggcg cgcctggcca tgccggccga tcggctgcgc ccgtccgccc
     4921 agcccatcct gctggtgcat ggctacggct gcaaccgcgc catctggtgg tggctgcggc
     4981 cgcgcctgga ggccgccggt cacgtggtgg ccacgctcag cctggagccg ccctggggcg
     5041 acatcgacgg ctttgccgag cagttgcagc agcgcatcga ggccgtgtgc gccgccaccg
     5101 gtgcgccgcg cctgaccctg gtggcgcaca gcatgggcgg gctggtgtcg cgcgcctgcc
     5161 tggcgcgcca cggtgctgcg cgggtggccg ctctgatcag catcgccagc ccgcacggcg
     5221 gcagccgcct ggcgcacctg ggcctgggcc gctgcgcccg gcagatgcag cccggctcgc
     5281 actggctggc gcggctggcg cagcagcgcg tgaacgtgcc cttcgtgtcg atccgcacgc
     5341 cgcaggacaa cttcgtcatg ccgcagcagc tgcagcgcca cgccgacgcg cgcgacgagc
     5401 cgctgcccgg cgtggggcac atggcggcgc tgtgcgacga gcgcaccttg cggctggtgc
     5461 tggcgcacac gaaactataa aaacaatagc tgccggcgct tgctaggcaa gcgccggagc
     5521 ccgaaaaagc attgaatacc aggggccggt gcgtgtgtcg gcgtcagaag tggatgccgc
     5581 gcatccagtt ctgcagggcc agctgatcgc gcgtcggcgc ggtcttttcg ccgctcgcgg
     5641 cggcctggga ctcgccgaac atgcgaaacg aggtgttcat caccgcctgc gccacgcgcg
     5701 gcatcagcgc gtgcagcacc tcgccggtga tgcccaggcg ggtcgccatg cgggtgggcc
     5761 ggtgcacgat ggcttcgcac accatgtcgg ccgcctcttc cggcgtcagg gtgggcgcct
     5821 ggtcgtacag cgcggtgggc gcgatcatcg gcgtcttgac cagcggcatg ttgatggtgg
     5881 tgaagtggat gccgcggtcg gcgaattccg aggccgcgca gcgcgtccag gcgtccagcg
     5941 ccgccttcga cgccacgtag gcggaaaagc gcggcgcgtt ggtcagcacg ccgatggagg
     6001 agatgttgat gatgtggccg gtgcgtcgct cggccatcgc cggcagcagg ttcaggttca
     6061 gccgcagcgc gccgaagtag ttgagctgca tggtgcgctc gaagtcgtga aagcgctcgt
     6121 aggagttttc gatggcgcgg cggatcgaac ggccggcgtt gttgatcagg atcgccaccg
     6181 gcccgtggtc gtgcaggatt tgctgtgcca ccgcgtccac ctgcgccagg tcggccagat
     6241 cggcttcata ggtgtgcagc ttcaggccca gcgcctcgaa ctcgctggcg gcggcgtcca
     6301 gcgcgggttt gccgcgcgcc atggtgacca cgatggcgcc ggcttcggcc agccgcagcg
     6361 ccaccgcctt gccgatgccc gaggaggcgc cggtgaccac caccacctga cccttgacgc
     6421 gcccgcccag gctgcggtcg atgaacaggt cggggtcgag gcagcgctcc cagtagtccc
     6481 acaggcgcca ggcgtagtct tccagcggcg gcacggcgat gcgcgtgccc ttgaggacgc
     6541 gctcggtttc gcggctgtcg aagcgcgtcg ggtagttgac gaagctcagg atgtcgtcgg
     6601 gcaggcccag ctccttcatc agcacgtcgc gcacgcgccg caccggcgtc agcgccagca
     6661 gcgccttgcg catgtatttg gggatgaagc ccagcagggc ggcgttgacg cgcaccgaca
     6721 tctgcggcgc atgaccggcg cgggcgaaga tgttgagcac gtcgcccacg cggcgtgggc
     6781 gcggatcgac caggtggaag cagcgcccgt cctcgccctt caggtgcgcg atgtggtcca
     6841 ccgcgtccac cacgtaatcc accggcacca ggttgatgcg cccgccctcg atgccgatgg
     6901 ccggcatcca ggctggcagc aggcggcgca gcttctggat cagcttgaag aagtagtacg
     6961 ggccgtcgat cttgtccatt tcgccggtgc gcgaatcgcc caccacgatg gccggtcggt
     7021 acacgcgcca gggaatgcgg cattcgttgc gcacgatgcc ttccgaatcg tgcttggtgc
     7081 ggaagtaggg gtgatccagc ccctcggcct gctcgaacat gtcttcgcgg aacacgccct
     7141 cgtacaggcc ggcggcggcg atcgagctga acaggtggaa atgcttggcg ccgatggcct
     7201 cggcaaagcg caccgcctga cgcgtgccct ccacgttgac ctggagctgt cgttcggcgt
     7261 cggcgctcag gtcgtagacg gccgccacgt gaaagaaatg tgtggtcttc ttgcccagct
     7321 tgcgccggtc ggccgccgac acgcccagca tgtcctgcgt cagatcgccc accaccgggg
     7381 tggcccgggt gtcgtcgcag ccccagaagt cgtacagcgc ctgcaggcgc tcggggtcgc
     7441 gctggcgcac cagaaagaag atgcgcgcgc cttcgcgctc cagcaatctg gccaccagcc
     7501 gcttgccgat gaaaccggtg gccccggtga tgaagtactg catgtctgcc tcctgcgtgg
     7561 ttctgatgct cagggcggca attctagaag tgccggcggc gtcggcagcg cgccacaggc
     7621 gcggtcacga atagtgtgtt tgctctaatg gcctggccta gacttcatct cagcgggttg
     7681 gaagaggtca atccgcagtc caccaccaca aggaaggagg ccaccatggc cagcaaactc
     7741 aagaccatct ccgacaacaa ccagctcgcc gccaccatca aggaatcgac ccagcagatc
     7801 tggctggccg gcctgggcgc ctttgccaag gcccaggaag agggcggcaa gatgttcgac
     7861 gccctcgtca aggagggcga gatgatgcag aagaagacgc gcgaagtggc cgacgcccgc
     7921 atcgccgccg tcgccggccg tgccgccgac acctgggacc gcctggagca ggtgttcgaa
     7981 gaccgcgtgg cgcgcgccct gggcagcctg ggtgtgccga gcaagaagga agtcgatgtg
     8041 ctgtccaagc gcatcgccga gctgaccgct gccgtgcagg aactggcgca gaacaagggc
     8101 aaggccgcgc cgcgcctgcc ggccgccaag ccggccgtgg ccaagccggc gccggccaag
     8161 aaggctgcgc ccgccaagaa ggccgcgcgc aagagcgcct gagcgtctga gtgccgcacg
     8221 ggcgcgcccg ggccggatgg cccagggcgt gtccagcggt cggcctgcca ggcggcgggc
     8281 ctgagacggt ggcgccaccg aaggcacggg gcgccaccga tggcttgggc atgggggaaa
     8341 acccacggcc tcagagttgg gggtaggtgc tttgtccaaa atcggattgg ctctggccgg
     8401 cggtggtccg ctcggcggca tttatgaagt cggcgcctgc gtggcgctgg aagaagccat
     8461 cgacggactg agcctggtgg atgccgacgt ctacgtcggc gtcagcgccg gcagcttcgt
     8521 ggcggcttcg ctggccaacg gcttcgagcc gcgccggctg gcgcgcatgc tgctgggcag
     8581 cgacggcagc caggcgttcg accccagcct gctgctgcgc ccggcgctgc gcgagtacct
     8641 gaaccgggcc cgcgccattc cgcggctggc gctgcaggcg gcgcgcgagt acctgggcaa
     8701 cccgtggcgg catgggctgt tcgaatcgtt ccagcgcctg tcgcgcgcca ttcccaccgg
     8761 cctgttcgac ggtgccggcg tggatgcggt gctgcgccgt cagttctccg aggttggccg
     8821 cagcaacgac ttccgccgcc tgaaggcgcg cctgtacgtg gtggcgaccg acctggattc
     8881 cggtcaggcg gtggcctttg gcgggcccgg gcgcgacgac gtgccgatct ccaccgccgt
     8941 gcaggccagc gccgccctgc cggggttgtt tccgccggtc gagatccagg gccggcacta
     9001 tgtggacggc gcgctgatca agaccctgca tgcctcggtg gcgctgcgcg acggcgtcga
     9061 tctgctgctg tgcgtcaacc cgctggtgcc gttcgacgcc cagctggccg agcagcacgg
     9121 cgccggccac cgacagctca gcgaaggcgg cctgccggtg gtgctggcac agacctttcg
     9181 cgccatcatc cgctcgcgca tgcgcgtggg catggaccgc taccagcacc agttcaagca
     9241 cgccgacgtg ctgctgttcg agccggcgca ggacgacgag gcgatgttct tcgccaacct
     9301 gttcagctac cgcgaccgcg cgcggctgtg cgaacacgcc taccagcgca cgcgcgagca
     9361 cctgtaccag cgccgcgacg agctggcgcc ggtgctggcg cgccatgggc tgcagctcaa
     9421 cctggaggtg ctgcgcgacc gcacacgcac cctgctgggg gcggagccgg cggccaccgc
     9481 cacgcagggc ctggcgcgca cgctcgacca gctgcagcac tggatcgacg cgcgccgcgc
     9541 ggcctgagct gagcacgcgg ggtggccggg ccgccgccgc gtcgtggtga aatggcggca
     9601 tgaacaagcc caagcgtcgc acgcccgagc ggatcctgga gaccgcgctg cgcctgttca
     9661 acgagttcgg cgagcccaac gtcaccacca cggtgatcgc cgacgagatg aacatcagtc
     9721 ccggcaacct gtactaccac taccgcaaca aggacgagat cgtcaatgcg ctgttcgcgc
     9781 gctacgagcg cgagatcagc gagctgctga cgctgcgcga caccgcgccg cgcgaggtgg
     9841 aggacgtgtg gttcttcctg cacctgctgt tcgagaccat ctcgacctac cgcttcatct
     9901 accgcgacat caacgacctg ctgacgcgca accgcctggt cgaaacgcat ttccagcgca
     9961 tcctggcgca caagcagcag accgccgtgg ccctgtgcca tggcctggca gcggccggcg
    10021 cgctggacgc cacgccggac gagatcgccg cgctggcggt caacatcacg ctggtcacca
    10081 ccttctggct gtcgttcgag cacgcgcgcc atccgcgccg cccgcccgac atcggccgtg
    10141 gggcgcacca ggtgctgtcg ctgacggcgc cctggctgcg cgagccggcg cgccggttgc
    10201 tggagaaact gtcactgtcc tacgtccatt ccacttcacg aggagacgcg cgatgagcaa
    10261 gaccgcctgg aaagcctttc cccatcccga cccggcctac acctacgccg gcaccgcgct
    10321 gaagaagcac tgggcgcgcc tgcaccaggg cgatgccgaa ccgtggccga ccgacagcgc
    10381 cgcgcaggcg gcctggcgcg cctaccacgc cggtgaattc gcgcaggcgg tggcgctggg
    10441 gctgaaggcc ggcaacgcca gcggcaccaa cgccgccaac aaggcggcca tgatccatgc
    10501 cgcctcggtc gaggacgacc aggcgcgcaa gctggcgctg ctgcaggagg tggcgcgccg
    10561 ctgcgaggcg ctgcagacca gccagccggg caacgtcaac gcctggtact accacggcta
    10621 cgcactgggg cgctacagcc agggcatctc ggtggccaag gcgctggccg agggactggg
    10681 cagcaaggtg cgcgagagcc tgcagaaggc catcgacctg gagccgcgcc acgccgatgc
    10741 ccacatcgcg ctgggcacct ggcatgccga gatcatcaac aaggtcggcg cggtggtcgg
    10801 tgggctgacc tatggcgcca agaaggacgc ggcggaaaag cacttcaaga ccgcgctggc
    10861 gctcaacccc gagtcgccga tcgccatgac cgaatacgcc aacggcctgg ccatgatgtt
    10921 cggcaagtcg cgcctgaagg aagccgagca gctctacgcc cgcgccgcgc agtgcacgcc
    10981 ggccgacgcc atggagcggc tggacgtgga ggtggcgcgc gccgaggtgg ctggctgacg
    11041 tcggtcaccc tctcctggat gatttttggt cagatcggcc tcaaagcctc tgccagcaag
    11101 cgccgcaagc tatcaagaat gtagcaaacg ggatcactcc gcggaccagt agaaggcgtt
    11161 gtgcgcctcc accagctcgg ggtcgtgcag gcactcgggc ggctccagca gcaccagcat
    11221 cagcagcttg tcgaattctt cggcaaagcg gctgacgatg cgctcggggt cgggcaccag
    11281 ccggcggtcg gtgatgaggc cgaactgcac ctggttggcg tacgacagga tcgacacacc
    11341 catgccgatg ttgcccgact gcggcaccca gaagaacggc tggcgcagcc gcgcgccggc
    11401 aaagtacagc ggctgctggg ccccgggtac gttggtcatc accgccgtgg ccttgttggc
    11461 cagaaagtcc agcagctgtt cctgcaccag cttggggccc atgccggcgg cgcccagcag
    11521 cgtcagcgtc agcgccggct ggtaggactg cttcatggcc tgcatgcgcc ggcgcgtttc
    11581 gtacaggcgc gccagcgggt tggcgacgcc gaccggcagc tccagcgtca ccatgccgaa
    11641 gcggttgccc agcttgccgg cgtctgcggc ctggcgcagg ttgaccggca ccagggcgcg
    11701 cagctccacg ccctcggtgg cgtcaccgca gtcggtcagg taggcgccca gcgcgccggc
    11761 cacggccgac agcagcaggt cgttgaccga gcagcccagc accttgccga cggccttgat
    11821 ctcgcgcagg tcgatcggct ccgaccaggc cacgcgcttg accagcccgg gcgtgccctt
    11881 gaagcgcgtg gtcgaatcgt tgggcatgac cagcagcttg gcggcctcgg ccgtcatgcc
    11941 gctgcccagg cgcgcgtagt ccagcgcctg ccaggggttg cgcagccagt cctgcgacat
    12001 cgcccacagc gaggtggacg cccgcaccga cgcctgcacg gcgcgcgaga tcgggtgcca
    12061 gaccatgccc cacagcgcgt cgtcgtggct gtggtgtggc ggcgtggcgg ccgccgccgg
    12121 cagcggcgcg ggggcgtcgt cggccatttc catgatcacc gccaccagcg cgatgccgtc
    12181 ggcgatgcag tggtgaaagc gcgtcaccag cgcctgcccg cccatgtggg tgtcgaccac
    12241 gtggatctgc cacagcgggc gcgccggcgg cagcggcgtg ctggccaggt cggcgacgaa
    12301 gcgctcgagt tcggccttgt cgcccttgcc cggcagcgcc gcgcggcgca ggtggttgtc
    12361 gaggtcgaac tcggggtcgt ccacccagga ccagccggtg gcgtcctcgc gcgcgaactg
    12421 gcgaaagcgc gcgtgcgaca gcaggcgctg ctcgatgcgc tggcgcaggc gggcgaagtc
    12481 cagcttgccg tcgaacagca tcacgcccag gatctgcatc aggttgttgg gccggtccat
    12541 ccgcagccag gcggtatcga cgggcgacat cttttcttgg gcggtcatgg tgattggcaa
    12601 gggcagggtg gtgggttcag gcgctgcgcg gggcaaaccg ctggtcgaag taatcgtgaa
    12661 tctcgcgctc gaagctggct ttgaacggca gaaacagcag ccccagccgg acctgcaccg
    12721 tcacctgtcg gcgctccagc gtgagctggc cgctgatgcc cgcgcgctgg aacgacagcg
    12781 tgccgtcgtc gtcccaggcg tactgcaggc cgtattcggc ctgcaggtcg gcggccaccg
    12841 cctcggcggc caggcgggcc ttcttggggc tcagcccatg ggcgcgggtg atgctgatct
    12901 ggctcatgcg caaagtgtaa agtgcgcggg gcggcggcgg ccatgggtgc gccgtcctgc
    12961 accaggcatt ggagcgaacc ccttcatgat cgacaagatt gcggcttccg tggccgaggc
    13021 cctggccgat gtgcgcgacg gcgccacggt gctgatcggc ggctttggca ccgccggcat
    13081 tccgggcgag ctgatcgacg gcctgatcgc gcagggcgcg cgcgagctga cggtcgtcaa
    13141 caacaacgcc ggcaacggcg accgcggcct ggccgcgctg ctggccgccg ggcgcgtgcg
    13201 caagatcatc tgcagctttc cgcgccaggc cgacagccag gtgttcgacg gcctgtaccg
    13261 cgccggccgc atcgagctgg agctggtgcc gcagggcaac ctggccgagc gcctgcgtgc
    13321 cgccggggcc ggcatcggcg ccttcttcac gcccaccggc ttcggcaccg agctggccaa
    13381 gaatcccgat ggcagcccca aggaaacgcg ccgcatcaat ggccgcgacc acgtgctcga
    13441 atacccgatc catggcgacg tggcgcttat ccgcgccgag cgcggcgacc gctggggcaa
    13501 cctgagctac cgcatggcgg cgcgcaactt cggcccggtg atggcgatgg ccgcgcgctg
    13561 caccgtagcc agcgtgcacg aggtggtggc gctgggccag ctcgaccccg aggccatcgt
    13621 cacgcccggc atcttcgtgc accgggtggt gcgcgtgccg cgcgtggcga ccgctgccgg
    13681 cggcttgcgg tctgcggcct gatcgggcgc cagcgcaagg agggtaagcg tgagcagcta
    13741 tcagaagcga agcaaggacg agctggcgcg gcgcgtcgcg caggacattc ccgacggcgc
    13801 ctacgtcaac ctgggcatcg gccagccgac ggcggtggcc aaccacattc cgcccgggcg
    13861 cgaaatcatc ctgcacagcg agaacggcat tctgggcatg ggcccggcgc cggccgccgg
    13921 gcaggaggac tacgacctca tcaacgccgg caagcagccg gtcaccctgc ggccgggcgg
    13981 cgccttcttc caccacgccg acagctttgc catgatgcgc ggcggccatc tggacgtgtg
    14041 cgtgctgggc gcctttcagg tgtcggtgac gggcgacctg gccaactgga gcaccggcga
    14101 ggccggtgcc attccggccg tcggcggcgc catggacctg gcggtgggcg cgcgccagac
    14161 ctgggtcatg atggacctgc tgacgcgggc cggcgaaagc aagatcgtgg cccgctgcag
    14221 ctacccgctg accgccatcg gctgcgtgcg gcgcatctac accgacctgg ccaccttcgc
    14281 ctgctcgccg cagggcctgc ggctgatcga tcgcgtcgag gggctgtcgc tcgacgaact
    14341 gcggcgcctg gtgggccagc cgtttgccgc ctgatgccgc gccacgcgcg gcgcgggttt
    14401 tcccgtcact gttttccaag gagactgcca tgacccaagc cttcatcgtc gacgccatcc
    14461 gcaccccctt cggtcgctac ggcggcgcgc tgtccggcgt gcgcaccgac gacctgggcg
    14521 ccatcccgat ccgggccctg atggcgcgcc acccgcgcgt cgactggcag gccgtgggcg
    14581 acgtgctgta cggctgcgcc aaccaggccg gcgaggacaa ccgcaacgtg gcgcgcatgt
    14641 cgggcctgct ggcgggcctg ccgatcgagg tgccgggcgc caccatcaac cgcctgtgcg
    14701 gctccgggct ggacgcggtc ggcacggcgg cgcgcgccat ccgcgccggc gaagcgcgcc
    14761 tgatgatcgc cggcggggtg gagagcatga gccgcgcgcc ctttgtcatg cccaaggccg
    14821 aaagcgcctt ctcgcgcgcc aacgcggtgt acgacaccac catcggctgg cgcttcgtca
    14881 accggctgat gaaggcgcag tatggcgtcg actcgatgcc cgagacggcc gagaacgtgg
    14941 cggtggacta ccgcatcgag cgcgaggcgc aggaccgcat ggccctggcc agccagatga
    15001 aggccgtggc ggcgcagcag gcgggccacc tggcgcgcga gatcacgccg gtgtcgatcg
    15061 cgcagaagaa gggcgatccg atcgtggtcg agcacgacga acacccgcgc gccaccagcc
    15121 tcgacaaact ggccgcgctc aagggcgtgg tgcggcccga cggcacggtg accgccggca
    15181 acgccagcgg cgtcaacgac ggcgcctgcg ccctgctgct ggccgacgag gccacggcca
    15241 gccagtacgg cctgacgccg cgcgcgcgcg tggtcggcat ggcggtggcc ggtgtggcgc
    15301 cgcgcgtcat gggcatcggc ccggcgccgg ccaccgagaa ggtgctgcag ctcaccggcc
    15361 tgaagctggc gcagatcgac gtcatcgaac tcaacgaagc ctttgccgcc cagggcctgg
    15421 cggtgctgcg cctgctcggc ctggccgacg acgacgagcg tgtcaacgcc tggggcggcg
    15481 ccatcgccct gggtcacccg ctgggtgcca gcggcgcgcg cctggccacc accgccatca
    15541 accgcctgca caccaccggc gggcgctacg cgctgtgcac catgtgcatc ggcgtcgggc
    15601 agggcatcgc cctggtgctg gagagggtct gaaaattgct attcaaataa tagctgtcgg
    15661 cgcttttcta gcaagcgctg gaggcctttt tgcctccaaa cgccgcggct gccccatcgc
    15721 ctggcgcgcc gtcgccggac cgatgccgcc ggtctcatcc cggtgcagcg ccgtcacgcg
    15781 ccctgcgcaa ggcgccggcg cttcagaccc gccagcccgg cagcgaccag ccgcgccaca
    15841 ccgacaccag ccgcatgccg gcggtgaaca gcacggtgac ggtcagcacc agccagggtg
    15901 gcagaccggc cgccagcaga cccacatgca gccagccgcc ggcaaacgac cacagggcgt
    15961 agggccggtg gtcgctgaag gcgctgggca gcaggttgca cagcacgtcg cgcagcacgc
    16021 cgccgaagca actggtgatc acgcccatca tcaccgccgc cagcgccggc aggcccaccg
    16081 ccaggcccag atcgacgccg atggcggtga acagccccag gccgatggcg tcgggccaca
    16141 ggatggcgcg ctcggtcagc gccagttcgc gctggcgcat caacgccatg gcggccaggc
    16201 acagcgccag cagggcccac acgaactcga tgtggcgcac ccagaaaaac gggcgctggt
    16261 ccagcagcag gtcgcgcagc gtgccgccac cgaaggccgc ggcaaaggcc accacgcaca
    16321 cacccaccac gtccagccgc tggcgggcgg cggcgatcac gcccgacagc gcgaaggcca
    16381 gcgtggccgc cacttccagc gcggtgcgca gcagttccga ccagccgggc ggcagcggtg
    16441 cgagcaggtt catgggcgtc gattgtgccc gccgcggcgg cgcccggccg cgccctcacc
    16501 gaactatcaa aatgatagct tgtggcgcag actggaacag cgccggcacc cgatctgacg
    16561 catgactccg atcaccgact ggggcttcta cgcggtcgcc gtgccggcgg tgctgctgct
    16621 gggcgtcagc aagagcggct tcggcgccgg cttcggctcg ctggcggtgc cgctgatggc
    16681 gctcagcgtc agcgtgccgc aggcggcggc gatcctgatg ccggtgctgc tggtgatgga
    16741 cctgctgggc atcgccgcct tccgcaagga cttcgaccgc gcgctgctgc gcttcatgct
    16801 gccctggggc ctgctgggca tcgtggtcgg cacgctgtcg ttccggctgc tgcaggcgca
    16861 ctgggtggcg ggcatcgtgg gcggcttcac gctgctgttt ctggcccagc gcctgctgtt
    16921 tgcgccacgc gccgacgccg cgccgccgcc gcgctgggcg ggggcgttgc tgacggcgac
    16981 ctcgggcttc accagcttcg tcgcccatgc gggcgggccg ccggtcaacg cctacgtgat
    17041 tccgctgggg ctggcgccgc tgcgcttcac cggcacgatg gcgttcttct tcttcgtcat
    17101 caacctggcc aagtggctgc cctacgcctg gctggggctg ctggacgggc gcaacctggg
    17161 cacgtcgctg gtgttgctgc cgctggcgcc gatcggcgtg tggatcggcg tgcgcattgc
    17221 gcggcgcatc gaccagcgcc tgttctaccg cttcgtctac ctgggtatgt tgctcaccgg
    17281 gctgaagctg ctgtgggatg cggtggccgc ctgagcgtca ctgaacctca gcgcccgcgc
    17341 cgcacccgca gcaactcgtc cagcatcagg ccgatggcgc cggcggtgat ggcggcgtcg
    17401 gccaggttga aggccgggaa atgcccgtgg tggaacagcg gcgccagaaa cggccagtgg
    17461 aagtccagga aatccaccac gtagccgtgc agcaggcggt cgatcacgtt gccgacggcg
    17521 ccgcccagga tcatggacag cgaaaaggcg aacagcttct gccccgggtg cgcgcgcagc
    17581 agccagacga tgaagcccgc caccgccagc gccagagcga cgaagaacca gcgctgccag
    17641 ccaccggcgt cggccaggaa cgagaacgcc gcgcccgtgt tgtgcgcccg cacgacgttg
    17701 aagaaggagg tgacggtggt gctgtcgccc agtcggtaat ggcccaggat cagctgcttg
    17761 gtgaactggt ccagcaccac caccacggcc gccagcgtca gccagacggc cagcgagccg
    17821 ccccgcgatg ccgcgcgcgc caccgtcagg ccaccgtgcg ggtctcgccc gcgccgaaca
    17881 ggttgctgac gcagcgcccg cacagggtgg ggtgttcggc gtgccggccg acgtcgggca
    17941 ggtaatgcca gcagcgctca catttggtgt ctttcgaggc gctgacgctg acggacaggg
    18001 cgccggcagc ttccagagcg atagcagaca cgatgcacac gaacttcagg tcggccccca
    18061 ggctggccag cagcgcgtgg tcgtcgggcg gcgcgcccag caccacgttg gcctgcagcg
    18121 acgagccgac cgcgccctgt tcgcgctgca cctcgatttc cttgttgacc tgctcgcgga
    18181 tggcgcggat gcgcgcccac ttggccagca gcgcggcgtc gggggcgtcg aagctccagt
    18241 actcttcgag gaagatcgag tcgctggtgc ccaccagctg ccacgcctcc tcggcggtga
    18301 agctgagaaa cggcgccatc cagcgcagca tggcctgggt gagctgccac agcgcggtct
    18361 gcgcgctgcg ccgtgcgggc gagtcgggcg cggtggtgta gaggcggtcc ttgagcacgt
    18421 cgaggtagaa gccgcccagg tcttcggagc agaacagctg cagcttggcg accacggggt
    18481 ggaattcgta gacctcgtag tgcgccagca gctcggcctg gaattcggcc gcgcgcgcca
    18541 gcagccagcg atcgagctcc agcagctgcg ccgccggcac ggcgtgcttg tgcgggtcga
    18601 agtcgctggt gttggccagc agaaagcgca gcgtgttgcg gatgcggcgg tagctgtcga
    18661 ccacgcgcgc caggatcttc tcgtcgatgc cgatgtcgcc cgaatagtcg ctggccgcca
    18721 cccacaggcg gatgatctcg gcgccgagct tctcgctcgt cacctgcggc gccaccgcgt
    18781 tgcccaggct cttgctcatc ttgcggccct ggccgtcgac cgtgaagccg tgcgtgagca
    18841 ggctgcggta gggcgcgcgg ccgcggatgg cgctggccag cagcagcgag gagtggaacc
    18901 agccgcggtg ctggtcgtgg ccctccaggt acatgtcggc ctcggggccg tgctcatggt
    18961 gttcggccgg atgcgtgccg cacagcacgt gccagaaggt cgagcccgag tcgaaccaga
    19021 cttccaggat gtcgctgctc ttggtgtagt gctgtgcgtc ctcggggccc aggatctcct
    19081 cggccgtgac gcggctccag gcctcgatgc cgccctgctc gacgatcgcc gccgcctggt
    19141 cgatgatctc catcgtgcgc gggtgcagcg cgtcggtttc cttgtgcagg aagaacggga
    19201 tcggcacgcc ccaggcgcgc tggcggctga tcacccagtc ggggcggccg gcgatcatgt
    19261 cgcgcagccg cgcctggccc gagggcgggt agaacgccgt ctgctcgatg gcgcgcagcg
    19321 ccgtggtgcg cagcgtctcg ggcgccttgt cggtggtgaa cacgccctcg ccctcgtcca
    19381 tgcgcacgaa ccactgcgag gcggcgcggt agatcaccgg cgatttgtgg cgccagcagt
    19441 gcgggtagct gtgctccagc ttgccgctgg ccagcaggcg gccggcctgg tccagcgcct
    19501 cgataatcgc cggctgcgcc ttccagatgt ccaggccgcc gaacagcgcg aagtccggcg
    19561 cgtagctgcc gccgccctgc accgggttca ggatgtcggc gtgcgccagg ccgtgggcga
    19621 cgcaggagtt gaagtcctcg acgccgtagg ccggcgccga gtgcacgctg ccggtgccgt
    19681 cgctgtcggt ggcgtagtcg gccaggtaca ccggcgcgag gcgccggtag gccgcgtcga
    19741 cctcgtacag cgggtgctcg aacgcaagac cgccgagctt gtcgcccatg gcggtggcga
    19801 tgatctcgcc ttgcaggccg tagcgctgca ggcagtcggc cacccgcgcc ttggccagca
    19861 gcagcaggcc gcgcgcggtg tcgaccagcg cgtactcgat gtgcggtccc aggttgagcg
    19921 cctggttggc cgggatggtc caggcggtgg tggtccagat gacggcgtag gccggcttgc
    19981 ccaggtgcgg caggccgaac gccgccgcca gcgcagccgg gtcgtgcgcc ttgaaggcca
    20041 cgtccaccgt ggtgctgcgg cggtcctggt actcgatctc ggcctcggcc agggccgagc
    20101 cgcaatcgaa gcaccagtag acgggtttca gcccgcggta gacgaagccg cgctcgatca
    20161 ggcgcttgaa cagccgcagc tcgcccgcct cgttggcgaa gtccatggtc ttgtagggct
    20221 tgtcccactc gcccagcacg cccagccgcc tgaagtcctt catctggctg gcgatttgct
    20281 cggtggcgta ggcgcggccc ttggcctgca tctcgtcgcg gctcaggtgg cggccgaact
    20341 gcttttcgat ctggttctcg atcggcaggc cgtggcagtc ccagcccggc acgtagtggg
    20401 cgtccatgcc cttcaactgg cgcgccttga cgatcatgtc cttgagcacc ttgttgaccg
    20461 cgtggccgat gtggatctgg ccgttggcgt agggcgggcc gtcgtgcagg atgaacttcg
    20521 cgcgcccggc gcgcgccgcg cgcaggcgcc ggtacagccc ctggtcttcc caggcctgca
    20581 cccagcccgg ctcgcgcttg ggcaggtcgc cgcgcatcgg gaagggcgtg tcgggcagat
    20641 tgagcgtctg gcggtagtcg gtcttggcgt cggtgctcat ggcgtgcaga gggagaaaac
    20701 ggggggtatg gatccgatcg gcctgttgcg caggcaggac gtgcgcaagc agctatcaaa
    20761 taaggagcgg ggcatgtggc gggcggggcg cccgccgccc cttcaaattc gcgccgccag
    20821 ccagtcgcgg gcggcggcgc aatcgcgcgc gatgccttcg gtcagcgccg ccaggctgtg
    20881 atagcgcaat tcatcgtgca gtttgtgcag cagatccacg cggatgattt taccgtaggc
    20941 cccctccggc cccagccggg ccggccagtc cagcacatgg gtttccagca gcacgcggcc
    21001 accgttgacg tcgtgcggat cgagcgaggg gcgcaccccc aggttggcca cgccgggcag
    21061 ggggcgggcg tccagcccat gcacctcgac cacgaaaatg ccgctggccg ccggcttcca
    21121 ggcgtgggcg cggcgcgaaa agcgcaggtt cagcgtgcga aagccgtcgc cgcggcccgg
    21181 cgccgcttcg gccagcgcgc ggcccagctt gcgcccatgc accacgtggc cgctgatggc
    21241 gtagggccgt cccagcagcg cggcggcggc ggccatgtcg ccggccgcca gcgcctcgcg
    21301 caccgccgag ctggacacgc gctggccgtg cacctcgtag ctcagcatgc gcgccacgtc
    21361 gaagcccagc cgctggccgg cggcgtccag catggcgtag tcgccggcgc gccgggcgcc
    21421 gaagcggaag tcgtcgccca ccagcacata gcgcacgccc aggccctgca ccagcacctg
    21481 gtcgatgaag tcctgcggtg gctggctggc cagcgcctgg ttgaacggca gcaccaccgc
    21541 ctggtcgatg ccgcagcgag ccagctcggc cagcttgtcg cggaaggtgg cgatgcgcgc
    21601 cggtgccagc tcggggcgct gctgcacggc ggcgaaatgg tcgcgcgggt gcggctcgaa
    21661 ggtcatcacg caactgggca cgccgcggtg cgccgcctcg ttgcgcagca gcgccagcat
    21721 ggcctggtgg ccgcggtgca cgccgtcgaa attgccgatg gtcagggcgc agccgggtgc
    21781 catgccgggg tggcgcaggc cgcggaagat cttcatgtcg gtacgttttt gatagcgcct
    21841 tccgcttgcc agacaagcgg aagcgcgcaa aagtattgtg aaatcggagt atattgcgag
    21901 ttgttcgaag gggcgccgtc gcgccacgga cagggcggcc ggccctggca atgccggcca
    21961 cacaaacagg gtttccgtgt caggaggtcg ttttgtgaag gtgctcaagc tgtcggcaca
    22021 ggggctgccg caatcgtgga tctcgctgga gcaggcggtg atccactacg ccgccgaaga
    22081 agtgcgctgg gaagcgggag gccagatcgc ggtgttccgc ggaggccaca acgccatcac
    22141 cggcgagcag tcgatcgtgc gcatcaacag catcatcggc acccacggcg cgccgcgcat
    22201 caaccccttc gagctgcgcc ccagcctgac caacgccaag ctgttcgcgc gcgaccgcaa
    22261 cctgtgcgcc tactgcggcc agcattacac cgaggccgaa ctgacgcgcg agcacgtggt
    22321 gccacaggcg cagcgcgggc gcgaccactg gatgaacgtg gtcaccgcct gccgcgcctg
    22381 caaccaccgc aagagcaacc gcacgcccga gcaggcgcac atgccgctgc tctacacccc
    22441 ctacgtgccc agtctgtggg aagacttcat cctgcgcaac cgccgcatcc tggccgacca
    22501 gatggagttc ctgatggccc acctgccacg cagctcgcgc ctgcacgcct gacggggcgg
    22561 gccgccggcg cggctatgct cgccccatgc cacgcctgat cttcttttgc ggccacgccg
    22621 gcaccggcaa gaccaccctg gcgcgtcgcg ccatcgcggc gctgatgcgg caggcccatg
    22681 cgcgctgcag cggcgaatcg ttctgcctgc tggacaagga cacgctgtac ggccagtaca
    22741 gcagcgccgt gatgggcgtg ctgaccggcg acccgaacga ccgcgacagc ccgccctacc
    22801 tgcagcacct gcgtcagccc gagtacgacg gtctgctggc caccgcgcgc gagaacctgg
    22861 cgctgggcgt gagctgcatc gtggtcggcc cgctgtcgcg cgaagtgcgg gcccaccgcc
    22921 tgaccgaccg cgcctggctg gcggtgggcg acgacgtgcg ggtgcacgtg gtgtgggtgc
    22981 atctggaaga agccgaggcg cgccggcgca tcaccgcccg cggcaatccc aacgacgcct
    23041 ggaagctggc gcactgggac gcctaccgca cgcggctgtt cgtgccgacg gcggccgagt
    23101 atccggagct gctgcagtac gacaacaccg acgcgccgcc cgcggccttc gaggggctgc
    23161 tggaggcgct ggcggatcga taggcgctcg ctgctggagc gctgccgtgg tgcagtgttt
    23221 cgcgctgtca ggcacttatc ctggaaacgg cagttgaccg cctgtcaact tcgggcagac
    23281 cgcatcgatg cggggtctgg cggctgcgat ggccttcacc tcctgggtga gcacgctgcg
    23341 cacccgcccg ccccgagcgc gccgtccaaa ggcgcgcagg cgccttcggg gcagcggcgc
    23401 tcatccttgc ctggcgcgcc gaccacggcg cgggcaggca cgtccaacgg ccgcttgcgg
    23461 gatgacaacc ggcgaatgac ctcgctgggc ttcgatgatg gacgagaccg cggtcagacc
    23521 ggcgccgccc atatgctgag ccactggtgg aacgcagcag gggtgtgacg cggctcaacc
    23581 cacgtccacc agcgcgcccg tcggcggctg gcgcggctcg tcggggcgca gttgccagaa
    23641 gatcgccgcc gagcccatgg tcatcagccc gatcgccacg aaggtggcac gaaacgccgg
    23701 cagcgactgc gcggcgccgg cgccacccag ccagtcgctg aaggcgtgca gcagggcggc
    23761 ggcgcaggtc acgcccaggc tcatgcccag catctgcacc atcgaaaaca ggctgttgcc
    23821 gctggccgcg cgctggggcg ccaggtcctt cagcgtgacc gcgttcatgg ccgtgaactg
    23881 ggtggagttg gcggcgccga acagcgccag ctgcacgatg cgcagccaca gcggctgctg
    23941 cggcgtcatc agggcgaagc cggcgatcat caggcccagc agcacggtgt tgcccatcag
    24001 caccggccgg tagcccagtc ggtggatgac gcgcgtgatg ggccgcttga tgcccatgct
    24061 ggccagcgcc accggcagca tcatcatgcc ggcctgcgcc ggtgaaaaac ccagcgccag
    24121 ctgcagcagc agcggcagga ggtacggcat ggcgcccgag ccgatgcgcg cgaacaggtt
    24181 gcccagcacg cccacgcggt agctggccac gccaaacagc gccggagcga acagtggcgc
    24241 cgggctgcgc tgcgcgtgca gccagtaggc cgccagcgcc gccagcccca gcaccatcag
    24301 cagcaccacc agcgtgcggg cccagcccag gccgcccagg ccgtccagcg acagcgaaat
    24361 gctgaccatc gccagcacca gcagcaggta gccgcccagg tcgaagcggc cggcctgggc
    24421 ggcgcgcagg ttgggcatgt ggcgccgggt cgccagcagg cccagcaggc cgaccggcag
    24481 gttgatgagg aagatccagt gccagctggc gacttccacc agccagccgc ccagggtcgg
    24541 accgatcagt gggccgacca ggccggggat ggcgacgaag ctcatggcct ccaggaagcg
    24601 ttcgcgcgga aacgcctgca gcaccgccag ccgccccacc ggtaacagca tggcgccacc
    24661 cacgccctgc agcacgcgcg cggcggtcag cagcgtcagg ctgggcgccg ccgcacaggc
    24721 ggccgagccc agggtgaaca gggcgatggc cagcgcaaac acgcgccggg tgccgaagcg
    24781 gtcggccagc cagccggtgg ccgggatcac cgccgccatc gtcagcgcat agctcaccac
    24841 caccgcctgc atgcgcagcg ggctttcgcc caggctgcgc gccatggccg gcagcgcggt
    24901 attgacgatg gtcgagtcca acgtctgcat gaaaaagccg atcgccacca gccacagcag
    24961 caggcgtggg ctgccgccgg cgggcgatgg gggcggtagg acgggtgaca tcgcgaacga
    25021 aaaaggcgcc gccgttgtcc ggcagcgcct gtgcggggga gggggactgg gccggacggt
    25081 caggcgaaca cgccggcggt gtcggtcatg cgctggctga cttggcccag gtggtgcagg
    25141 gtgtcgcccc agctcatttc cagctgggtc aggctcttga agtagtggct gcccacgtat
    25201 tcgtcggtca cgccgatgcc accgtgcagc tgcaccgact gctggccgac gaagcgcagc
    25261 gcctggccca gctgcacctt ggcgcgcgac agcgcctggc ggcgctcggc ctcgggggcg
    25321 ccgagcttga gcgtggcgta gtagctcatg ctgcgcgcca gctccagctg catcttcatg
    25381 tcggcgacgc ggtggcgcaa ggcctggaag ctggcgatga ccacgccgaa ctgcttgcgc
    25441 tggttcatgt agtcgaccgt gagcttgagc gtctggtcca tcgcacccac gccgtacgcg
    25501 caggcgcagg cgatgccgac gtcggcggcg tgcatcaggg cggtcaggcc gtcggtggtg
    25561 accagttgcg ccggcgcgcc gttgaactgg acgtcggcgg cgcgcgagcc gtcctgcgtc
    25621 aggtagccgc gcgtgttcac gccctgggcg ccgcgctcga tcaggaacag cgccagcttg
    25681 ccgttcagct gggccggcac cagccaggcg tcggcctggt cgccggcggc cacgatgtgc
    25741 ttcaggccgg tgacggtgtg accgccgccg gtggcgctgg cctgggcttc gcaggtgtcc
    25801 agccggtagc gtgccttgcg ctcctgatgg gccagcacca ccagcgcctc gccgctggcg
    25861 atgcgcggca gccaggcctg gcgcacggcg tcgctgccgt gggcgcccag ggtggtgctg
    25921 accacgaagg tctgcgtcag cggctcgacc acgatgccgc ggcccagctc ttccagcacc
    25981 accatggcct cgaccgggcc catgcccagg ccgccgtggt cttcgccgac gcagatgccg
    26041 gccaggccca gttcggccag ttcgccgtag gcggcgcggt caaagccgcc ggcggcgacg
    26101 atggcgcggc ggcgctcgaa gtcgtagctc ttttcgaccc acttggcgac ggcgtcgcgc
    26161 agggccagct ggtcgtcgga aaaatcgaaa tccatgtctc tgactccttg tgttctgttc
    26221 agcccagcac ggtctgcgcg acgatgttgc gctgcacttc gttggagccg ccgtagatgg
    26281 tggtcttgcg gtagttgaag tagctggcgg ccagcgtggc gttggccacg gcgccgccgg
    26341 ggaagccctg cagcccggcg gcggcagcct ggtcgccctg ccagccggct tccatggctt
    26401 cccagatgta gggcaggctg aacgggccgg cggccagcat catgagttcg gtgtagcgct
    26461 gctggatttc gctgcccttg atcttgagca gaccggcgat gtcgagcgcg ttgcggccgg
    26521 acttttcggc cgacagcacg cgcagcacca gcatctccag cgccacgatg tccacttcca
    26581 gcagggcgat ctggtcgcga aagcgcgcgt cgtcccacac gccctcggcc cgggcgatgc
    26641 gcttgaggcg ctcgagttcg cgcttggcgc gattgacgtc ggcgatgttg gtgcgctcgt
    26701 ggctgagcag gtgcttggcg taggtccagc ccttgttctc ctcgccgatc aggtt
//
LOCUS       DAOQTZ010000028        26630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2130_length_26630_cov_14.055616, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000028 DAOQTZ010000000
VERSION     DAOQTZ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 26630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26630
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2130_length_26630_cov_14.055616"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PKN51_05110"
     CDS             complement(<1..166)
                     /locus_tag="PKN51_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNT84511.1"
                     /translation="MKQITAIIKPFKLEEVREALAEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDF"
     gene            complement(184..1842)
                     /locus_tag="PKN51_05115"
     CDS             complement(184..1842)
                     /locus_tag="PKN51_05115"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNT84512.1"
                     /translation="MSLKICVAQLNLVVGDLPGNARQIIDAAHTAHAQGARLLLTPEL
                     AISGYIAEDLFLRPAFIAACDDALNRVARETAGLGGLTIVVGHPSAVAPVDDVGPRCA
                     NAASVLCEGRRVAHYAKRLLPNYEVFDERRYFTPGQGSCVFTVDGVRVGLLICEDAWF
                     DEPAAATRQAGAELLAVINASPYHQGKGAEREAAMARRARASGLPLVYANLVGGQDEV
                     VFDGRSLAIGADGQLAGRAASFKENLFFVQASRAAAAIELKADMAPEPTPEAELWEAL
                     VLGLRDYVDKNGFAGVALGLSGGLDSALVLALAVDALGAARVRTLMMPSPYTAGMSVE
                     DAHEMARRLGVRHDELSIRPAFETLRATLAPLFAGRGEDVTEENIQARVRGVLLMALS
                     NKLGHLILTTGNKSEYAVGYCTLYGDMCGGFAPIKDVAKTTAYRLARWRNAHDPHGTG
                     ANPMPERIITRAPSAELREGQTDQDSLPPYEVLDAIVARYVEDNASIADLLAEGFAAA
                     DVERVTRLIKASEYKRQQAAVGTRVTRRSFGKDWRYPMTNRYRV"
     gene            1845..3275
                     /locus_tag="PKN51_05120"
     CDS             1845..3275
                     /locus_tag="PKN51_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HNT84513.1"
                     /translation="MQAGGRPGEYRNAAQRPADAAPALQVLDTPAALDAARWNALLAR
                     QPSPTPFMRHEYLLALHASGSAVPHTGWTPRFMLLWAGSELIAACPLYLKSHSFGEYV
                     FDWAWANAHAEHGLPYYPKAVVAVPFTPVPGTRLLARDQAARAALIAAVRDWCADQRL
                     SSLHLLFGDETDLAACEGAGLMRRSQVQFHWKNGAPPRSAAAPAALPPVGALAALGRP
                     GGGEMVPPRSAAAPAALPPVGALAALGRPGGGSDAPAERPFADFDDFLAALTQDKRKK
                     IRQERRRVAQAGVRFRWAAGNDITAEDWAFFYRCYRRTYLEHGNAPYLTPAFFAHMAR
                     HMADVWVLFTAERESRPIATSLIAVGARPESAGGSNDAYNGAVAYGRYWGALERVDGL
                     HFEACYHQPLEWCIHHGVARFEGGAQGEHKMARALLPVATHSAHWLAHPGFADAVARF
                     LARESLAVDGYLGQLGERSPFRRPDG"
     gene            complement(3295..3612)
                     /locus_tag="PKN51_05125"
     CDS             complement(3295..3612)
                     /locus_tag="PKN51_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84514.1"
                     /translation="MKLSIARLSIGAAVLGAAVGAQAACYTVLGPKGQILSESPNPPV
                     DMSLPLHLSVPERFGPGAHMVFGLLDAPCGQEVELHDGGALTPAALRPDSRRVRTPRR
                     DRE"
     gene            complement(3678..5180)
                     /locus_tag="PKN51_05130"
     CDS             complement(3678..5180)
                     /locus_tag="PKN51_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HNT84515.1"
                     /translation="MLKKIVLGLLAVLLALAAGVAGNTWRQSSRQLQVAPLAPLAIDE
                     PAVADRLAQAVRLQTVSSASDAQLNADQFRALHALLQTQFPRVHATLKREIVNELSLL
                     YTWEGADPKANAIVLLAHQDVVPVAPGTEGDWQQPPFAGLVKDGFIWGRGSWDDKGNL
                     LAQLEAVERLLAEGYRPPRTIHLAYGADEEVGGHRGAARIAELLKSRGVQADFVLDEG
                     LLVLDGVMPGLKQPAALIGVAEKGYLSVRITVTAPPGHSSMPPRPGSSAIALLSAGLD
                     RLEHAPMPAGIRGVAGEMFGTLAPEMGGLARVALSNLWLFGPLVQKQLEGGASTNAML
                     RTTTALTMLQAGNKDNVLPGRAEATVNFRMLPGETREQVLRHVRQQLARVVPEGQFEV
                     TALPGGKEASQVAPTDSAPYQMLARTVREVFPDALVAPGLMVAGTDSVHYEAISRHVF
                     KFSPIRAVSDDLPRFHGTDERLGVTHYANAIRFYHRLIPQLGVPGSSVGR"
     gene            complement(5268..6668)
                     /locus_tag="PKN51_05135"
     CDS             complement(5268..6668)
                     /locus_tag="PKN51_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HNT84516.1"
                     /translation="MTEALTIAVVGHTNAGKTSLLRTLTRRVDFGQVSDRPGTTRHAE
                     AVALRLDGREAVRFIDTPGLEDSVALLDFLARQSGDSRPERVRAFLAGPEARASFEQE
                     AKVLRALLEQAGCAMLVIDTREPVLPKHRAEIEILASCAKPVMPVLNFVRDGASRLAD
                     WHRALEESNLHARAEFDVVAPFTGAEQRLYADLATLLPARRAQLQDIVEALAQAERER
                     RLAACRVIASTLIDVAAQRRSLSAEAFADPEQQQAWVRDFQAQTGRHARRAVDELLTL
                     YAFGADDAELTELPLPGGRWEDDLFNPELLKQAGRRLGLGAMIGAGMGAVADVALAGL
                     SLGAATTLGATLGGAISGGWQPLWRKLQNRLDGVQELTVEDPVLWLLAERLLALARAL
                     AQRGHAAQQRLRLGAEAAGALGDDGQALIDALSAARGHPAWERGVAGALDDAERARLL
                     EALAGALRPALERQPA"
     gene            complement(6661..8106)
                     /locus_tag="PKN51_05140"
     CDS             complement(6661..8106)
                     /locus_tag="PKN51_05140"
                     /inference="COORDINATES: protein motif:HMM:NF022518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HNT84517.1"
                     /translation="MRVTTSTSPSTPVAAAVLHETVRRIEDAGPLDDAQALAQAHRMP
                     GGRGAQVLARSQLLAERLGLPVEWARWRRLGAGVLLGLALLLALADLGLARAVLGQGR
                     SINAVAAFVSLLGLHALTLVLWLLGLLLPAGRRGGAPLGRLALWLSARLPLERGPHAL
                     TLLRALGAVLGRHGLLVWLTGAISHAVWALGFVIMGAVLVFGFAFHAYALTWESTILS
                     ASFFQRFVQITGALPALLGFAVPDAAAVTQAGHAAVGATAIAQSDWAWWLIGCVVAYG
                     LLPRVLLALLCGWRWRAGQARLAQPDMADPYVRRIVARLDALEPPPQVIDAEQRSAHP
                     TYAVRAGPPGAPGSLAVLGFELPPEQPWPLPGLPTGLSAGSEPPQRIAGSTADRQAAL
                     ARLQASRPESLLLVVHGPSSPDRGTARFVREAQALCHRMALLALGAGGSSGAGRWRAW
                     LDSEGFEAVALVEQAQAAIEWIASGDAAHHD"
     gene            8285..8956
                     /locus_tag="PKN51_05145"
     CDS             8285..8956
                     /locus_tag="PKN51_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HNT84518.1"
                     /translation="MCNRYESPQAREIERLWHVGRDQPSRWWDEVLHPRGQGPFIRRA
                     REGGGRELVVGQWGLIPWFAPQARLAYSTNNARSEELASKASYKQPWARGQRCIIPAR
                     SFDEPCWETGRNVWWRFRRADGQPWGLAGLWNTWIDRASGEVHESYTMLTINADQHPL
                     MRRMHKPDPKLAPDQQDKRSVIAIEPGDVPQWLEGTLDEARALLRAPAPELFDAGPAV
                     QLPAL"
     gene            9275..9673
                     /locus_tag="PKN51_05150"
     CDS             9275..9673
                     /locus_tag="PKN51_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNT84519.1"
                     /translation="MTPHPIDAWHALVRSHDPRGLDALLADDAVFISPVVHTPQRGKA
                     VTRAYLSAAFQVFVNPTFRYVREIRGPSDAMLEFETEIDGILVNGVDIIRWGADGRIA
                     EFKVMVRPLKAIQLIHQRMAALLAAAPRSA"
     gene            complement(9756..10736)
                     /locus_tag="PKN51_05155"
     CDS             complement(9756..10736)
                     /locus_tag="PKN51_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HNT84520.1"
                     /translation="MPIAVQIERPGGAEEMKLVDLPVGQPGPGQVRIRHHAIGLNFID
                     VYQRTGLYPLPMPLTLGMEGAGVIEAVGEGVTHLKPGDRAAYAGNPPGSYSQVRVMPA
                     LTVCRLPDDIDFDTAAAMMLKGLTAQYLLRRTLPVEGLQPGDHILWHAAAGGVGLIAC
                     QWAKALGYRLIATAGSEQKCALALANGAQAAINYRAEDFAQRVAQITDGQGVKVVYDS
                     VGKDTWEGSLQCLRPFGLMASFGNASGPVPPFAPAVLAPKCLYVTRATLFTHIATRER
                     CQAMADELFAVVRSGQVRIHIDQRFALQDVQAAHRALEARQTTGSTILTL"
     gene            10824..11726
                     /locus_tag="PKN51_05160"
     CDS             10824..11726
                     /locus_tag="PKN51_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT84521.1"
                     /translation="MAHPPLTLKNAALLTLPPLLWAGNAVLGRLVVDLVPPATLNFLR
                     WLLAFFILLPLAGWVLRPGSGLWPHWRRFAVLGLLGVGLYNTLQYLALHTSTPVNVTL
                     VASSMPLWMLALGRLFFNAPVRRPQLLGALLSMLGVTVVLARGDLANLARLQLVPGDV
                     YILAATLAWALYSWLLARPAEPAAIRADWAALLLAQVTFGLGWSGLLAGGEWLLTDAQ
                     IRWGWPLAGALVFVACGPAVLAYRCWGLGVQRAGPALAGFFSNLTPVFAALLSTVLLG
                     EAPRDYHALAFGLIVLSIWVSSRR"
     gene            11784..12302
                     /locus_tag="PKN51_05165"
     CDS             11784..12302
                     /locus_tag="PKN51_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84522.1"
                     /translation="MIPNLSEDELMLLATLPQAIGSCVATAAGSGVLGTGKELFASGQ
                     AVMAGLKDYPDNALIQALVPDPSAADKHGELAQARATRDWALARLKERNIRTPAQMAA
                     LTLEDARAVALLLDSRVEPAQAAQYRQWAMSVAEKVAMAATEGGFLGFGGTRLSEAEQ
                     ALIAQLRAALGA"
     gene            complement(12303..13730)
                     /locus_tag="PKN51_05170"
     CDS             complement(12303..13730)
                     /locus_tag="PKN51_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNT84523.1"
                     /translation="MTATTCTAAAGDDFIENITFDELAIGQSARLVRTLSQSDIQAFA
                     AVSGDTNPAHLDPAYADATLFQGVIAHGMWGGALISAVLGTQLPGPGTIYLEQALRFS
                     RPVRVGDTLTVTATVLSKDDARKRVELDCQVSNQHGERVLSGVAKVLAPTEKVRQPRT
                     PLPQLRLFDPEARLKAWVAQAAGTPAVHCGVVHPCHPGVLEGVLRAAHAGLIVPQLIG
                     PPERIHAAAEQAGLNLQGVEIIEAPHSDAAAVVAAQRAADGRLEALIAGSLALSELLD
                     AVRAQTALHTRRRLSHVFRLDVPGHARPLWLTDAMLHAAPTLADKADIARNAIDLLRV
                     MGVEQPRVAVLSASEQVDAAVPSTIDAAALCKMVDAGQIGGGLIDGPLAFDSAVSPAS
                     AQGKHPRSAVAGQADVLLAPDMETGSMLARQLLYLAGATACGVIVGARVPITVVTHRL
                     DSANAYLASAVLAKRLALHHRLVRP"
     gene            complement(13794..14591)
                     /locus_tag="PKN51_05175"
     CDS             complement(13794..14591)
                     /locus_tag="PKN51_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT84524.1"
                     /translation="MDLGIAGKWALVCGASKGLGLGCAQALAGEGVNLLMVARGESAL
                     AQAAAEIRAAGADRAGVSVEFVAQDITTPEGRAAVLARRAEFDIVVTNAGGPPTGDFR
                     DWDRQAWIRALDANMLTPIELIRATLDGMLARGFGRIVNITSSAVKAPIDILGLSNGA
                     RSGLTGFVAGLARNPQIAARGVTINNLLPGKFDTDRLAATFQGAAQKSGQPLEAVRAA
                     QQAQIPARRFGTPAEFGAICAFLCSQQAAYLTGQNILPDGGAYPGSF"
     gene            complement(14637..15551)
                     /locus_tag="PKN51_05180"
     CDS             complement(14637..15551)
                     /locus_tag="PKN51_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT84525.1"
                     /translation="MAPAGQAPAATNTVAFGLLLAALGAIGFSGKAIIVKLAYRWGVD
                     AITLLMYRMLMALPFFVLMAWWGGRGRPALSRTETRAVLGLGFCGYYLASMLDFIGLQ
                     YITASLERLILYLTPTLVLLCGWALRARPLSAGHLLATAVSYGGALLVFGMEVRDTPG
                     AATAWGAFWVFLSAVSYALYLFFSGEFVARIGSLRLVGLASVVASLCCIAHFLLVNPW
                     QAARVAEPVLWLSLLNSTLCTALPVLLVMMGVERIGASLAAQVGMIGPLSTIAMGFVV
                     LDEPLTPWLAVGTLLVVAGIYLFGRAGR"
     gene            complement(15575..16906)
                     /locus_tag="PKN51_05185"
     CDS             complement(15575..16906)
                     /locus_tag="PKN51_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HNT84526.1"
                     /translation="MPTAHPLRAELHHELHARPPEALSAPLAVLHCVMWADAAARQQS
                     RAHLASLLAAAGLPAPPEGANFHRAQWPELTLRWEQHTEFVAWTFLRPMAAADLQALQ
                     AGAPRDDADGVPAAWLAALPGQCLTRLRLWAVPREAVDGAAAVRALFDASSVTGSTVI
                     SHSSDLHTDMRLQADGSLRMLVLVGEHAPGRVTPRRLGRLVQRLLDIETYRMAALMGL
                     PAARQSVAWLASGEDELARLAQAVQHAAPDDEAALLDRLTRLAAQLEGHYAATHTRFS
                     ASAAYFSLLDQRLAEIAETRIEGMQSLHDFMQRRLMPAMHTCRSAERRQAALSARIER
                     VSGLLRTRVEVEQQQSSRELLAGMNQRQELQLKLQSTVEGLSVAAISYYVLGLLGYLL
                     KGAQRVGWPFSAEASIAAAVPLVVLAVWWSLRRLHHRIVGHGAPTTDRSSP"
     gene            complement(17038..18549)
                     /locus_tag="PKN51_05190"
     CDS             complement(17038..18549)
                     /locus_tag="PKN51_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNT84527.1"
                     /translation="MELLEHLALGFSVAFTLQNLMYAFIGCLLGTLIGVLPGVGPVAT
                     IAMLLPTTYALPPVAALIMLAGIYYGAQYGGSTTAILVNLPGEASSVVTVIDGHQMAR
                     KGRAGPALAAAAIGSFIAGCFGTLVLAGFAVPLTEVAFKFGPAEYFSLMVLGLIGAVV
                     LASGSLIKAVGMIVLGLLLGLVGTDVNSGVARYSFDIPELTDGIGFIAIAMGVFGYGE
                     IIANLSHAQGKREVFDVKLQNLYPSKEDWKLMTPSIVRGTLLGSALGILPGGGAVLSA
                     FAAYTIEKKTKLKSGEMPFGKGNIRGVAAPESANNAGSQTSFIPLLTLGIPPNAVMAL
                     MVGAMTIHNIQPGPQVMTSNPELFWGLIASMWIGNAMLVILNLPLIGLWIKLLTVPYR
                     WLFPAIVLFCAIGVYTTNNNTWDIWMMGWFGIVGYIFHKLSMEPAPLLLGLILGPMME
                     ENLRRALLLSRGDWSILVTRPLSASLLALAAALLVIVLLPAIKKSREEAFVED"
     gene            complement(18569..19057)
                     /locus_tag="PKN51_05195"
     CDS             complement(18569..19057)
                     /locus_tag="PKN51_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNT84528.1"
                     /translation="MRIKSQRDFFAGLLYVVIGGAFAIGANNYTIGNAARMGPGYFPL
                     LVGILLAILGAIIVVKSLVVETPDGDPIGPWAWKPLIYIILANFLFGLLLGGVPSLGI
                     PSLGVMIGILVLTLVAAKAGPEFKLKEVIILAIVLAAGSWLVFIWGLNLQMPVWPSFI
                     SG"
     gene            complement(19261..21075)
                     /locus_tag="PKN51_05200"
     CDS             complement(19261..21075)
                     /locus_tag="PKN51_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNT84529.1"
                     /translation="MKRSTRPTPLTVVDGYRGPVHWRLLLQWLREDAVISAAEAARVH
                     ARCAQAESAQHALVRLAAVGITRAADGAAMDVEALTRYLAERAGLAYLRIDPLRVDVG
                     KVSDTMSASYAERHKVLPVQVTPAEVVVATSEPFIDDWVAEVERQSRRQVRRVVANPL
                     DIKRYTAEFFALARSVRVAQKQGGGSAGAASFEQLVELGRTGRQLDANDQGVIQVVDW
                     LWQYAFDQRASDIHLEPRREQGVIRFRIDGVLHPVYQVPAGVMNAITARIKLLGRMDV
                     VERRRPQDGRIKTRSPGGEEIEMRLSTLPTAFGEKLVMRIFDPDTAVKDLAALGFTPH
                     DAARWEALVQRPHGIILVTGPTGSGKTTTLYSTLRRVATEEVNVSTIEDPIERIDPAL
                     NQTQVQPQLDFSFAEGVRALMRQDPDIIMIGEIRDLETAEMAVQAALTGHLVFSTLHT
                     NDAPSALTRLMELGVPPYLLSATILGVLAQRLVRTLCPHCRQPDEQGSQPLLADVIKP
                     WKISAADYLPYKPVGCVDCRMTGFRGRMGIYELLTVSDAFKDRVRADVDLAALRQQAI
                     ADGMRPLRLAGALRAAEGLTTLQEVIAATPPLEPRAHS"
     gene            complement(21072..21830)
                     /locus_tag="PKN51_05205"
     CDS             complement(21072..21830)
                     /locus_tag="PKN51_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HNT84530.1"
                     /translation="MLKRQQPTFSEVNLSEHDGVRYLHLGTEWVQGSMRIDAPFEIDL
                     EYVQRMMAWLLFVPEDSVAGRHAMQLGLGAGAITKFCYKKLRMTCTAVELNPRVADVC
                     RAWLRLPPDDERLRVVLADAAEEIRRDEWLGTVDALAVDLYDDEAAAPVLDSAAFYAD
                     CRRLLTEDGCMTVNLFGRSSSFERSLACISEAFGAQAVWAFKATREGNTVVLAQRSAT
                     RPKRPLLQQRAEEIEARWGLPARKWLKVFKAIEK"
     gene            22019..22843
                     /locus_tag="PKN51_05210"
     CDS             22019..22843
                     /locus_tag="PKN51_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNT84531.1"
                     /translation="MNTQDILHFITTQGAEFGLKLLTAIAAWIVGRWLIGLAVGLMGK
                     IFERGGKIDTTLAHYLKSITSVLLNIVLILAILDIFGVKTTSFAALLAGAGLAIGAAW
                     SGMLGNFAAGVFMQVLRPYKVGDFISAGGVVGTVTELGLFATTLTTPDNVMTVVGNGK
                     IFGDNIQNFSALPVRRVDCVAKVANSVNPLDAMARLKPVIAAIPNVAASPAPDIEILE
                     FTPEGPKLCVRPYTHTDHYWQVYFDTHKAIVETFGAAGYPVPETPVAYRSAPSGVA"
     gene            complement(22968..24329)
                     /gene="glmM"
                     /locus_tag="PKN51_05215"
     CDS             complement(22968..24329)
                     /gene="glmM"
                     /locus_tag="PKN51_05215"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNT84532.1"
                     /translation="MSRQYFGTDGIRGTVGQAPITPDFALRLGHAVGRVLRRTQPDAT
                     VLIGKDTRISGYMLESALESGFNSAGVDVVLLGPVPTPAVAYLTRAQRAELGVVISAS
                     HNPYPDNGIKFFSARGTKLPDDWEREVEAVLAEVPQWVSADALGRARRLDDAAGRYIE
                     FCKSTFANDLTLRGLKIVVDAAHGAAYQIAPKVFHELGADVLAIGCAPDGLNINDAVG
                     ATHPEALVRAVAEHGADYGIALDGDADRLQMVDASGRLFNGDELLYVLAIDRLARDAS
                     LRGAGAPRPPALAGVVGTLMTNLAVEQALRARGFDFVRAKVGDRYVLEELVQRGWILG
                     GEGSGHLLALDKHTTGDGLISALQVLQACAMQGRTLAQLLEGVALCPQVLINVRLQAG
                     QDWRHYTRLTDATRAVEAELGASGRVLIRASGTEPLLRVMVEAVDAAQAQRCAERLAA
                     AAG"
     gene            complement(24391..25248)
                     /gene="folP"
                     /locus_tag="PKN51_05220"
     CDS             complement(24391..25248)
                     /gene="folP"
                     /locus_tag="PKN51_05220"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNT84533.1"
                     /translation="MIWQTSRFRIDLAEPRVMGIVNVTPDSFADGGQHPDAASALRHC
                     ERLLKDGAQLLDIGGESTRPGSPPLPLAEELARVLPVLRGAVQLGVPVSIDTYKPEVM
                     QAALDLGVDIVNDVWALRQPGAAQLVARHPSCGIVLMHMHGEPRSMQTAPMEGDAPPQ
                     VLSFLEHAAQGLQALGVDRTRIVVDPGIGFGKTVAQNFSLLARQRELLAAGWPLLVGW
                     SRKSSIGAALQGDAPAPPPPAERVLGSVAAALLAVERGARIVRVHDVRETVQALAVWR
                     QARQTLLKS"
     gene            complement(25434..>26630)
                     /gene="ftsH"
                     /locus_tag="PKN51_05225"
     CDS             complement(25434..>26630)
                     /gene="ftsH"
                     /locus_tag="PKN51_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNT84534.1"
                     /translation="RVRDMFENAKKNAPCIIFIDEIDAVGRQRGAGLGGGNDEREQTL
                     NQMLVEMDGFETNLGVIVVAATNRPDILDAALLRPGRFDRQVYVTLPDIRGREQILNV
                     HMRKIPIGQDVSPAIIARGTPGMSGADLANLCNEAALMAARRNARVVEMQDFEKAKDK
                     ILMGPERKSMVMPEEERRNTAYHESGHALIGKLLPKCDPVHKVTIIPRGRALGVTMSL
                     PENDRYSYDKQYMLNQISMLFGGRIAEEVFMNQMTTGASNDFERATQIARDMVMRYGM
                     SEAMGPMVYAENEGEVFLGRSVTKTTNISEETMQKVDAEVRRIIDEQYALARKLIEDN
                     QDKMHAMAKALLEWETIDGEQLDDIMAGREPRPPKDFTPRTPPSGGTGGTPAEAKPAD
                     PAPTAA"
BASE COUNT         3938 a         9404 c         9367 g         3921 t
ORIGIN      
        1 ggaagtccac cacgtattcg gcgccacggt acagctcggt atggcccttc tggcgaccga
       61 agcccttgac ttcggtgacc gtcaggccgg tgacgccgac ctcggccagc gcctcgcgca
      121 cttcctccag cttgaatggt ttgatgatgg cggtgatctg tttcatggat gctcctgaag
      181 cggtcagact cggtatctgt tggtcatagg ataacgccag tccttgccaa agctgcggcg
      241 ggtgacgcgg gtgccgacgg ccgcctgctg gcgcttgtat tcgctggcct tgatgagccg
      301 agtgacgcgc tccacatcgg ctgcggcaaa gccctcggcc agcagatcgg cgatgctggc
      361 attgtcctcg acgtagcggg ccacgatggc gtccagcacc tcgtagggcg gcaggctgtc
      421 ctggtcggtc tggccctcgc gcagttcggc gctgggcgcg cgcgtgatga tgcgctcggg
      481 catcgggttt gcgcccgtgc cgtgggggtc gtgcgcgttg cgccagcgcg ccagccggta
      541 ggcggtggtc ttggcaacgt ccttgatcgg cgcgaagcca ccgcacatgt cgccgtacag
      601 cgtgcagtag ccgaccgcgt attcgctctt gttgccggtg gtcaggatca ggtggccgag
      661 cttgttggac agcgccatca gcagcacacc gcgcacgcgc gcctggatgt tttcttccgt
      721 cacgtcctcg ccgcggccgg caaacagcgg cgccagggtg gcgcgcaggg tctcgaaggc
      781 cggccggatc gacagctcgt cgtgccgcac gcccaggcgc cgcgccatct cgtgcgcgtc
      841 ctccacgctc atgccggcgg tgtaggggct gggcatcatc agggtgcgca cccgggcggc
      901 acccagggca tcgaccgcca gtgccagcac cagggccgaa tccaacccgc cggacagccc
      961 cagcgccacg ccggcgaagc cgttcttgtc cacgtagtcg cgcagaccaa gcaccagcgc
     1021 ctcccacagc tcggcctccg gcgtcggctc gggggccata tctgctttca attcgatagc
     1081 tgccgccgca cgactggcct gcacgaagaa cagattttcc ttgaaactgg cggcacggcc
     1141 ggcgagctga ccatcggcgc cgatggccag cgagcggcca tcgaacacca cctcgtcctg
     1201 cccgcccacc aggttggcgt acaccagcgg aagaccgctg gcgcgagcgc ggcgcgccat
     1261 cgccgcctcg cgctcggcgc ccttgccctg gtggtagggc gaagcgttga tgaccgccag
     1321 caactcggcg ccggcctggc gcgtggcggc ggcgggttca tcgaaccagg cgtcttcgca
     1381 gatcagcagg cccacccgca cgccatcgac cgtgaacacg caactgccct gtccgggggt
     1441 gaagtagcgc cgctcgtcga acacttcgta gttgggcagc aggcgcttgg cgtaatgcgc
     1501 cacccgccgg ccttcgcaca gcacgctggc ggcattggcg cagcgcggcc cgacgtcgtc
     1561 gaccggcgcc accgcgctcg gatgccccac cacgatggtc agcccgccca accccgccgt
     1621 ctcgcgggcc acccgattca gggcatcatc gcaggcggcg atgaaagccg ggcgcaggaa
     1681 caggtcttcg gcgatgtaac cgctgatggc cagctccggc gtcagcagca ggcgtgcgcc
     1741 ctgggcgtgg gcggtgtggg cagcgtcgat gatctgcctg gcgttgcccg gcaggtcgcc
     1801 caccacgagg ttgagctggg cgacgcagat cttgagagac atgaatgcag gcaggtggca
     1861 gaccgggcga gtatcgtaac gcggcgcagc ggccggccga cgcggcgccc gcgctgcagg
     1921 tgctggacac gccggccgcg ctcgacgccg cgcgctggaa cgccctgctg gcgcgtcagc
     1981 ccagccccac gcccttcatg cgccacgaat acctgctggc gctgcacgcc agcggcagcg
     2041 ccgtcccgca caccgggtgg acgccgcgct tcatgctgct gtgggccggc agcgagctga
     2101 tcgccgcctg tccgctgtac ctgaaaagcc attccttcgg cgaatacgtg ttcgactggg
     2161 cctgggccaa cgcccacgcc gaacacggcc tgccctacta ccccaaggcg gtggtggccg
     2221 tgcccttcac gcccgtgccc ggcacgcgcc tgctggcgcg cgaccaggcg gcgcgcgcgg
     2281 cgctgatcgc cgccgtgcgc gactggtgcg ccgaccagcg gctgtcgtcg ctgcacctgc
     2341 tgttcggtga tgaaaccgat ctggccgcct gcgaaggcgc cggcctgatg cggcgcagcc
     2401 aggtgcagtt tcactggaaa aatggagcgc ccccacgctc cgcggctgcg cctgctgcgc
     2461 tgccccccgt gggggccctc gccgccttgg ggcggcccgg cggcggcgag atggtgcccc
     2521 cacgctccgc ggctgcgcct gctgcgctgc cccccgtggg ggccctcgcc gccttggggc
     2581 ggcccggcgg cggctcggac gcgccggcgg agcgtccgtt tgccgatttc gacgacttcc
     2641 tggccgcgct gacgcaggac aagcgcaaga agatccgcca ggagcggcgc cgcgtggccc
     2701 aggccggggt gcgttttcgc tgggctgcgg gcaacgacat cacggccgag gactgggcct
     2761 tcttctaccg ctgctaccgg cgcacctacc tggagcacgg caacgcgccc tacctcacgc
     2821 cggccttctt tgcccacatg gcgcgccaca tggcggacgt ctgggtgctg ttcacggccg
     2881 agcgcgaaag ccgcccgatc gctaccagtt tgatagctgt cggcgcacgc ccagaaagcg
     2941 ctggaggctc aaacgatgcc tataacggag cggtggccta cggccgctac tggggcgcgc
     3001 tggagcgcgt ggatggcctg cacttcgaag cctgctacca ccagccgctc gaatggtgca
     3061 tacaccacgg cgtggcgcgc ttcgaaggcg gcgcccaggg tgaacacaag atggcgcgcg
     3121 ccctgctgcc ggtggccacg cacagcgccc actggctggc gcaccccggc tttgccgacg
     3181 ccgtggcgcg ctttctggcg cgcgaatcgc ttgccgtcga tggctatctg ggccagttgg
     3241 gcgagcgctc gccgttccgg cgaccggacg gctgaggcgc gcgcgcgccg ccgctcattc
     3301 gcggtcgcgg cgcggggtgc gcacgcgccg gctgtcgggg cgcagcgccg ccggggtcag
     3361 cgcgccgccg tcgtgcagct cgacttcctg cccgcagggc gcatcgagca gaccgaacac
     3421 catgtgggcg cccgggccga agcgctccgg cacgctcagg tgcagcggca gcgacatgtc
     3481 caccggcggg ttcgggcttt ccgacaggat ctgacccttg ggccccagca cggtgtagca
     3541 ggcggcctgc gcgcccacgg cggcgcccag cacggcggcg ccgatcgaca gcctggcaat
     3601 ggaaagtttc atggtggtcc cccgaggttg aacggccggc gtcggcaccg gccccgctga
     3661 cgctgagtgt atgccgctca gcgcccgacg ctgctgcccg gcacccccag ttgcgggatc
     3721 aggcggtgat agaaacggat cgcgttggcg taatgcgtca cgcccagccg ttcgtcggtg
     3781 ccgtgaaagc gcggcaggtc gtcgctgacg gcgcggatgg gcgagaactt gaacacatgc
     3841 cggctgatgg cctcgtagtg caccgaatcg gtgcccgcca ccatcagccc cggcgccacc
     3901 agggcgtcgg gaaacacctc gcggaccgtg cgcgccagca tctggtaggg ggccgaatcg
     3961 gtgggcgcga cctgcgaggc ttccttgccg ccaggcaggg cggtcacttc gaactggcct
     4021 tcgggcacca cccgagcgag ctgctggcgc acgtggcgca gcacctgctc gcgcgtttcg
     4081 cccggcagca tgcggaagtt gaccgtggcc tcggcgcggc cgggcagcac gttgtccttg
     4141 ttgccggcct gcagcatggt cagcgcggtg gtggtgcgca gcatggcgtt ggtgctggcc
     4201 ccgccttcca gctgcttctg caccagcggg ccgaacagcc acaggttgga cagcgccacg
     4261 cgcgccaggc cgcccatctc gggcgccagg gtgccgaaca tctcgcccgc cacgccgcgg
     4321 atgccggccg gcatcggcgc gtgctccagc cggtccaggc cggcgctcag cagggcgatg
     4381 gcgctgctgc cgggcctggg cggcatggac gagtgcccgg gcggcgccgt caccgtgatg
     4441 cgcaccgaca gatagccctt ttcggccacg ccgatcagcg ccgccggctg cttcaggccg
     4501 ggcatcacgc cgtcgagcac cagcagcccc tcgtccagca cgaagtcggc ctgcacgccg
     4561 cgcgacttca gcagctcggc gatgcgggcc gcgccacggt ggccaccgac ctcctcgtcg
     4621 gcgccatagg ccagatggat ggtgcgcggc ggccggtagc cctcggccag caggcgctcg
     4681 accgcctcca gctgggccag caggttgccc ttgtcgtccc aggagccgcg gccccagatg
     4741 aaaccgtcct tcaccagccc ggcaaacggc ggctgctgcc agtcgccctc ggtgccgggc
     4801 gccaccggca ccacgtcctg gtgcgccagc agcacgatgg cgttggcttt cgggtcggcg
     4861 ccttcccagg tgtagagcag gctcagctcg ttgacgattt cgcgcttgag cgtggcgtgc
     4921 acgcgcggaa actgcgtctg cagcagcgcg tgcagggcgc ggaactgatc ggcgttgagc
     4981 tgcgcgtcgc tggccgaaga cacggtctgc agccgcaccg cctgcgccag ccgatcggcc
     5041 accgcgggct cgtcgatcgc cagcggcgcc agcggcgcca cctgcagctg gcgcgacgac
     5101 tggcgccagg tgttgcccgc cacccccgcc gccagcgcca gcagcacggc cagcaggccc
     5161 agcacgatct tcttcagcat cagcggtctc ccggaattcg tgagcgatgg taaccgcagc
     5221 gcggcgcacg gcggcgcccc attcagcggc cgcggccgca ggcaagatca agccggctgg
     5281 cgctcgagcg ctggccgcag cgcgccggcc agcgcctcca gcaggcgggc gcgctcggca
     5341 tcgtccagtg cgccggcgac gccgcgctcc cacgccgggt ggccgcgggc ggcgctcagc
     5401 gcgtcgatca gcgcctgccc gtcgtcgccc agggcgccgg cagcttcggc gcccaggcgc
     5461 agccgctgct gcgccgcatg gccacgctgc gccagcgcgc gcgccagcgc cagcaggcgc
     5521 tcggccagca gccacagcac cgggtcttcc accgtcagct cctgcacgcc atccagacgg
     5581 ttctgcagct tgcgccacag cggctgccag ccgccggaga tagcgccacc cagggtggcg
     5641 cccagggtgg tggcggcgcc cagcgacaga ccggccagcg ccacgtcggc caccgcgccc
     5701 atgccggcac cgatcatggc gcccaggccc aggcgcctgc cggcctgctt gagcagctcg
     5761 ggattgaaca ggtcgtcttc ccagcggccg ccgggcagcg gcagctcggt cagctcggcg
     5821 tcgtcggcgc cgaaggcgta cagcgtcagc agctcgtcga cggcgcggcg cgcgtggcgg
     5881 ccagtctggg cctgaaagtc acgcacccag gcctgctgct gctcggggtc ggcaaacgcc
     5941 tcggccgaca ggctgcggcg ctgggcggca acgtcgatca gcgtgctggc gatgacgcgg
     6001 caggccgcca ggcggcgctc gcgctcggcc tgcgccagcg cttcgacgat gtcctgcaac
     6061 tgcgcgcggc gcgccggcag cagggtggcc aggtcggcgt acaggcgctg ctcggcgccg
     6121 gtgaagggcg ccaccacgtc gaactcggca cgcgcgtgca gattgctttc ttccagcgcg
     6181 cggtgccagt cggccaggcg gctggcgccg tcgcgcacga agttgagcac cggcatcacc
     6241 ggcttggcgc agctggccag gatttcgatt tcggcgcggt gcttgggcag caccggctcg
     6301 cgcgtgtcga tcaccagcat ggcgcagccg gcctgctcca gcagggcgcg cagcaccttg
     6361 gcctcctgct cgaagctggc gcgcgcctcg ggcccggcca ggaaggcgcg cacgcgctcg
     6421 gggcgcgaat cgccgctctg gcgcgccaga aaatccagca gcgccaccga atcttccagc
     6481 cccggcgtgt cgatgaagcg caccgcctcg cgcccgtcca gccgcagcgc caccgcctcg
     6541 gcatggcgcg tggtgccggg gcgatccgac acctggccaa agtcgacccg gcgcgtgagc
     6601 gtgcgcagca gcgaagtctt gcccgcgttg gtatggccga cgacggcgat ggtgagcgct
     6661 tcagtcatgg tgtgcggcgt cgccggatgc tatccattcg atagctgctt gcgcttgctc
     6721 cacaagcgcc acagcctcaa aaccctctga atccagccag gcgcgccagc gccctgcccc
     6781 gctcgaccca cccgccccca gcgccagcag cgccatgcgg tgacacagcg cctgcgcctc
     6841 gcgcacgaag cgcgccgtcc cgcggtcggg gctggacggg ccatgcacca ccagcagcag
     6901 cgactcgggc cggctggcct gcaggcgcgc cagcgcggcc tgccgatcgg cggtgctgcc
     6961 ggcgatgcgc tggggtggct cgctgcctgc agacaaaccg gtgggcaagc cgggcagcgg
     7021 ccagggctgc tcgggcggca gctcgaaacc cagcaccgcc agcgaacccg gcgcgccggg
     7081 cgggccggca cgcacggcgt aggttggatg ggcgctgcgc tgctcggcgt cgatcacctg
     7141 cggcggcggc tccagcgcgt cgagccgcgc cacgatgcgg cgcacgtacg ggtcggccat
     7201 gtccggctgc gccagccgcg cctgcccggc gcgccagcgc cagccgcaca gcagcgccag
     7261 cagcacccgc ggcagcaggc cataggccac cacgcagccg atcagccacc aggcccaatc
     7321 gctctgggcg atggcggttg cacccacggc ggcatggccc gcctgcgtca ccgccgccgc
     7381 gtcgggcacg gcaaagccca gcagcgccgg cagcgcgccc gtgatctgca cgaagcgttg
     7441 gaagaacgac gcgctcagga tggtgctctc ccaggtcagc gcgtaggcat gaaaggcaaa
     7501 gccgaacacc agcaccgccc ccatgatgac aaagcccagc gcccacaccg catggctgat
     7561 cgcgcccgtc agccacacca gcaggccgtg gcggcccaac accgcgccca gcgcacgcag
     7621 cagcgtcagc gcatgcggcc cgcgctccag cggcaggcgc gccgacagcc acagcgccag
     7681 ccgccccagc ggcgccccgc cgcgccgtcc ggccggcagc aacaggccca gcagccacag
     7741 caccagcgtc agcgcgtgca gccccagcag actgacgaac gccgccaccg cgttgatgct
     7801 gcgcccctgg cccagcaccg cccgcgccag ccccagatcg gccagcgcga gcaacagcgc
     7861 cagccccagc agcacccccg cgcccagccg ccgccagcgc gcccattcaa ccggcagccc
     7921 cagccgctcg gccagcaact gcgagcgcgc cagcacctgc gcaccccgtc cgcccggcat
     7981 gcggtgcgcc tgcgccagcg cctgggcgtc gtccagcgga ccggcgtctt cgatgcgacg
     8041 cacggtttcg tgcaggactg ctgcggcgac gggcgtgctg ggactggtgg atgtggtgac
     8101 gcgcatgagg gcgtgaagtg ctgcaaagca tgatagcgag ggacctgcac cgcaaacgga
     8161 ttggccggcg caacgaccga cgcccatcgc ccggtccgat cacgcttcag cgcatgcgca
     8221 ctaactgatc cacctcgatc atcgaccgct gcccgactgc accgttgcgc gtatcctgcg
     8281 cctggtgtgc aaccgctacg agtcccccca agcccgcgaa atcgagcgcc tctggcacgt
     8341 gggccgcgat cagcccagcc gctggtggga cgaggtgctg cacccgcgtg gacaggggcc
     8401 gttcattcgc cgcgcgcgtg agggcggcgg gcgcgagctg gtggtgggcc agtgggggct
     8461 gattccgtgg tttgcgccgc aggcgcgcct ggcgtacagc accaacaacg cgcgcagcga
     8521 ggagctggcg agcaaggcct cgtacaagca gccctgggcg cggggccagc gctgcatcat
     8581 cccggcccgc tcgttcgacg agccgtgctg ggagacgggg cgcaacgtgt ggtggcgctt
     8641 tcggcgcgcc gacggccagc cctggggtct ggcagggctg tggaacacct ggatcgaccg
     8701 cgccagcggc gaggtgcacg agagctacac catgctgacg atcaacgccg accagcaccc
     8761 gctgatgcgc cgcatgcaca agcccgaccc caagctggcc cccgaccagc aggacaagcg
     8821 cagcgtgatt gccatcgaac cgggcgacgt gccgcagtgg ctggagggca cgctggacga
     8881 ggcgcgcgcc ctgctgcgcg cgccggcgcc ggagctgttt gacgcgggcc cggccgtgca
     8941 gctgcccgct ttatgagcgc gtttcgcctg ctgcgcccgt ccagcaaacg ctaacagcta
     9001 ttgttttgac aagacaagcg gggctgatgg ggtgaattgg gatcagagag gtgaagtagg
     9061 gtcaaatcat gattttcatg agagttttag ccttcaccct tcacccatca agcgcgatca
     9121 gctaccaatt cgatagtgct cagtagatag tgattgaccg catcagtcag tcgccagccc
     9181 cgcctccagg ccgggctcac cctgcgggcg acgggccggg cggccggctt ggcgggcttg
     9241 tccgggcgcg cgcgcgccgc gctaccattg ccgcatgacg ccacacccga tcgacgcctg
     9301 gcacgccctt gtccgctctc acgacccgcg cgggctggac gcgctgctgg ccgatgacgc
     9361 ggtgttcatc tcgccggtgg tgcacacgcc ccagcggggc aaggcggtca cgcgggccta
     9421 tctgtcggcg gcgtttcagg tgttcgtcaa cccgacgttc cgctacgtgc gcgagattcg
     9481 cggcccgtcg gacgcgatgc tggagttcga gaccgagatc gacggcatcc tggtcaacgg
     9541 cgtggacatc atccgctggg gcgccgacgg ccgcattgcc gagttcaagg tgatggtgcg
     9601 cccgctgaag gcgatccagt tgatccacca gcgcatggcg gccctgctgg ccgccgcccc
     9661 gcgatcggcc tgacttggca tgcgcaggcc cgccggggcc agggcgcttg tgccccttgg
     9721 cgcgcaagcc gcaacccagg cggccgcgcg ccggcttaca gcgtcaggat ggtcgatccg
     9781 gtggtctggc gcgcttccag ggcgcgatgg gcggcctgca cgtcctgcag ggcaaagcgc
     9841 tggtcgatgt ggatcctgac ctggccggag cgcaccacgg caaacagctc gtcggccatg
     9901 gcctggcagc gctcgcgcgt ggcgatgtgg gtgaacaggg tggcgcgcgt gacgtacagg
     9961 catttgggcg ccagcacggc gggggcaaac ggcggcaccg gaccgctggc gttgccaaag
    10021 ctggccatca gcccgaacgg gcgcaggcat tggagcgagc cttcccaggt gtctttgccg
    10081 accgagtcgt agaccacctt gacgccctgg ccgtcggtga tctgggcaac ccgctgggcg
    10141 aagtcttctg ctctgtaatt gatagctgct tgcgcaccat tggcaagcgc cagcgcgcat
    10201 ttttgttctg aaccggcggt ggcgatgagg cggtagccca aggccttggc ccactggcag
    10261 gcgatcaggc ccacgccgcc ggcggcggcg tgccagagga tgtggtcgcc cggctgcaga
    10321 ccctccaccg gcagggtgcg gcgcagcagg tactgcgcgg tcaggccctt gagcatcatg
    10381 gcggcggcgg tgtcgaagtc gatgtcgtcg ggcagccggc acacggtcag cgcgggcatc
    10441 acgcgcacct ggctgtagct gccgggcggg ttgccggcgt aggcggcgcg gtcgccgggc
    10501 ttgaggtggg tgacgccctc gcccaccgcc tcgatcacgc cggcgccttc catgcccagg
    10561 gtcagcggca tgggcagcgg gtacaggccg gtgcgctggt agacgtcgat gaagttcagg
    10621 ccgatggcgt ggtggcggat gcgcacctgg ccggggccgg gctggcccac gggcagatcg
    10681 accagtttca tttcctcggc gccgccgggg cgctcgatct ggacggcgat gggcatgggc
    10741 gatctcctcg ggggcgggtg cgtgaaagcc gcgatgttgc cacgccggcg cgggggatgc
    10801 ccggcgtggc aacatcgccc tacatggccc atccacccct gacgctgaag aacgccgccc
    10861 tgctgaccct gccgccgctg ctgtgggcgg gcaacgcggt gctggggcgg ctggtggtgg
    10921 atctggtgcc gccggcgacg ctgaactttc tgcgctggct gctggccttc ttcatcctgc
    10981 tgccgctggc cggctgggtg ctgcgcccgg gcagcggcct gtggccccac tggcggcgct
    11041 ttgccgtgct gggcctgctg ggcgtggggc tgtacaacac gctgcagtac ctggcgctgc
    11101 acacgtcgac gccggtcaac gtgacactgg tggcgtcgag catgccgctg tggatgctgg
    11161 cgctggggcg gctgttcttc aacgccccgg tgaggcgccc gcaactgctg ggggcgctgc
    11221 tgtcgatgct gggcgtgacg gtggtgctgg cgcgcggcga tctggccaac ctggcgcggc
    11281 tgcagctggt gccgggcgac gtgtacatcc tggcggccac actggcctgg gcgctgtatt
    11341 cgtggctgct ggcgcggccg gccgaaccgg cggcgatccg cgccgactgg gccgccttgc
    11401 tgctggcgca ggtgaccttc gggctgggct ggtcgggcct tctggcgggc ggcgaatggc
    11461 tgctgaccga cgcgcagatc cgctggggct ggccgctggc gggggcgctg gtgttcgtgg
    11521 cctgcgggcc ggcggtgctg gcctaccgct gctggggcct gggggtgcag cgcgccgggc
    11581 cggcgctggc gggctttttc tcgaacctga cgccggtgtt cgcggcgctg ctgtcgaccg
    11641 tgctgctggg cgaagctccg cgcgactacc acgcgctggc cttcgggttg atcgtgctgt
    11701 cgatctgggt gtcgtcgcgg cgctgagccg gcccgccgcc ggtctacact ggcctgccct
    11761 acccgatccc tgaaaggcgc cccatgatcc ccaacctgag cgaagacgaa ctgatgctgc
    11821 tggccacgct gccgcaggcc atcggctcct gcgtcgccac ggcggcgggc agcggcgtgc
    11881 tgggcacggg caaggagctg ttcgcctcgg gccaggcggt gatggcgggg ctgaaggact
    11941 accccgacaa cgcgctgatc caggcgctgg tgcccgaccc gtccgccgcc gacaagcacg
    12001 gcgagctggc gcaggcgcgc gccacgcgcg actgggcgct ggcgcgcctg aaggagcgca
    12061 acatccgcac gccggcgcag atggcggcgc tgacgctgga ggacgcgcgc gcggtggcac
    12121 tgctgctgga cagccgcgtc gagccggcgc aggcggcgca gtaccgccag tgggcgatgt
    12181 cggtggccga gaaggtggcc atggcggcca ccgaaggcgg ctttctgggc tttggcggca
    12241 cccgcctgag cgaggccgag caggcgctga tcgcgcaact gcgcgccgcc ctgggcgcct
    12301 gatcagggcc gcaccagccg gtggtgcagg gccaggcgct tggccagcac ggccgaggcc
    12361 aggtaggcgt tggcgctgtc cagacggtgc gtcaccacgg tgattggcac gcgcgcgccc
    12421 acgatcacgc cgcaggcggt ggcgccggcc aggtacagca gctggcgcgc cagcatggag
    12481 ccggtttcca tgtccggggc cagcagcacg tcggcctggc ccgccacggc cgaacgcggg
    12541 tgcttgccct gcgccgacgc cggcgagacg gcgctgtcga aggccagcgg cccgtcgatc
    12601 aggccgccgc cgatctggcc cgcgtcgacc atcttgcaca gcgccgcggc gtcgatggtg
    12661 gaaggcacgg cggcatcgac ctgctcggac gccgacagca ccgccacgcg cggctgctcc
    12721 acccccatga cgcgcagcag gtcgatggcg ttgcgcgcga tgtcggcctt gtctgccagc
    12781 gtgggcgccg cgtgcagcat ggcgtcggtc agccacagcg gccgggcgtg gcccggcacg
    12841 tcgagccgaa acacgtgcga caggcgccgg cgcgtgtgca acgcggtctg cgcgcgcacg
    12901 gcgtcgagca gctcgctcag ggccaggctg ccggcgatca gggcttcgag ccgaccatcg
    12961 gccgcgcgct gcgccgccac gacggccgcc gcgtcgctgt gcggggcctc gatgatctcc
    13021 acgccctgca ggttcagccc cgcctgctcg gcggcggcat ggatgcgctc gggcgggccg
    13081 atcagctgcg gcacgatgag cccggcatgg gcggcgcgca gcacgccctc cagcaccccg
    13141 ggatggcacg ggtggaccac gccgcagtgc accgcgggtg tgccggcggc ctgcgccacc
    13201 caggccttga gccgcgcctc ggggtcgaac aggcgcagct gcggcagtgg ggtgcgcggc
    13261 tgacgcactt tttcggtcgg cgccagcacc ttggccacgc cggacagcac ccgctcgcca
    13321 tgctggttgc tgacctggca gtccagctcg acccgcttgc gggcgtcgtc cttgctcagc
    13381 acggtggcgg tgacggtcag tgtgtcgccc acccgcaccg ggcgcgaaaa gcgcagggcc
    13441 tgctccaggt agatggtgcc cgggcccggc agctgggtgc ccagcacggc cgatatcagt
    13501 gccccgcccc acatgccgtg ggcaatcacg ccctggaaca aggtggcgtc ggcgtacgcc
    13561 ggatcgaggt gggccgggtt ggtgtcgccc gagacggcgg caaaggcctg gatgtcggac
    13621 tggctcagcg tgcgcaccag gcgcgcgctc tgcccgatgg caagctcgtc gaacgtgatg
    13681 ttctcgatga agtcgtcgcc cgccgcagcg gtgcaggtgg tggcggtcat gtgaaggttc
    13741 ggttgcaggc tggcccggca cggccgcgcc ggcgtggcgc cgggccgatg acgtcagaag
    13801 ctgccgggat aggcgccgcc gtcgggcagg atgttctgcc cggtcaggta ggccgcctgc
    13861 tggctgcaca gaaaggcgca gatggcgccg aactcggccg gcgtgccgaa gcggcgcgcc
    13921 ggaatctggg cctgctgggc ggcgcgcacc gcctccagcg gctggcccga cttctgcgcc
    13981 gcgccctgga aggtggcggc caggcggtcg gtatcgaact tgccgggcag caggttgttg
    14041 atggtcacgc cgcgggcggc gatctgcggg ttgcgcgcca gcccggcgac gaaacccgtc
    14101 agcccggagc gcgcgccgtt ggacaggccc aggatgtcga tcggcgcctt caccgcgctg
    14161 gaggtgatgt tgacgatgcg cccgaagccg cgcgccagca tgccgtcgag cgtggcacgg
    14221 atcagctcga tgggcgtgag catgttggcg tccagcgcgc ggatccaggc ctggcggtcc
    14281 cagtcgcgaa agtcgccggt gggcggcccg ccggcgttgg tgaccacgat gtcgaactcg
    14341 gcacgccgcg ccaacaccgc cgcgcggcct tcgggcgtgg tgatgtcctg tgccacgaat
    14401 tcgacgctaa cgcccgcccg gtcagcgccg gcagctctta tttctgcagc agcctgggcc
    14461 agcgccgact cgccccgcgc caccatcagc aggttcacgc cctcgccggc cagcgcctgt
    14521 gcgcagccca ggcccaaccc cttgctggcg ccgcacacca gcgcccattt gcctgcaatg
    14581 cccagatcca tgcgtgctct ccttggtcaa tcacgtgtgg cggcggctgc ggcgcgtcag
    14641 cgcccggcgc gcccgaacag atagatgccg gccaccacca gcagcgtgcc gaccgccagc
    14701 cagggggtca gcggctcgtc cagaacgaca aaacccatgg cgatggtgga cagcggcccg
    14761 atcatgccca cctgcgccgc caggctggcg ccgatgcgct ccacccccat catcaccagc
    14821 agcaccggca gcgccgtgca cagggtcgaa ttgagcagcg acagccacag caccggctcg
    14881 gccacgcgcg ccgcctgcca cgggttgacc agcaggaaat gcgcgatgca gcacaggctg
    14941 gccaccaccg aggccaggcc caccagccgc aggctgccga tgcgggccac gaattcgccg
    15001 ctgaagaaca ggtacagcgc atacgaaacc gccgacagga acacccagaa cgccccccag
    15061 gcggtggcgg ccccgggcgt gtcgcgcacc tccatgccga acaccagcag cgcgccaccg
    15121 tagctgaccg cggtggccag cagatgcccg gcgctgagcg ggcgcgcgcg cagggcccaa
    15181 ccgcacagca gcaccagcgt gggcgtgagg tacaggatca gccgctccag gctggcggtg
    15241 atgtactgca ggccgatgaa gtccagcatg ctggccaggt agtagccgca aaaacccagc
    15301 cccagcacgg cccgcgtttc ggtgcgcgac agcgccggcc gcccgcgccc gccccaccag
    15361 gccatcagca cgaagaacgg cagcgccatc agcatgcggt acatcagcag cgtgatggcg
    15421 tccacgcccc agcggtaggc cagcttgaca atgatcgcct tgccgctgaa gccgatcgcc
    15481 cccagggccg ccagcagcag cccgaatgct accgtatttg tagctgctgg cgcttgtccg
    15541 gcgggcgcca gagccggttt ggatgcagaa tgcgtcacgg cgatgaccgg tccgtggtcg
    15601 gcgcgccgtg cccgacgatg cggtggtgca gtcggcgcag cgaccaccac acggccagca
    15661 ccaccagcgg caccgcggcg gcgatgctgg cttcggcgct gaacggccag ccgacgcgct
    15721 gcgccccctt gagcaggtag cccagcaggc ccagcacgta gtaggagatc gccgccaccg
    15781 acagcccctc gaccgtcgat tgcagcttca gctgcagctc ctggcgctgg ttcatgccgg
    15841 ccagcagctc gcggctggac tgttgctgct cgacctcgac gcgcgtgcgc agcaggccgc
    15901 tgacgcgctc gatgcgcgcc gacagcgcgg cctggcgccg ttcggccgaa cggcaggtgt
    15961 gcatggccgg catcaggcgc cgctgcatga agtcgtgcag cgactgcatg ccttcgatgc
    16021 gggtttcggc gatttcggcc agccgctgat cgagcaggct gaagtaggct gcgctggccg
    16081 aaaagcgcgt gtgcgtggcg gcgtagtggc cttccagctg cgccgccagg cgcgtcaggc
    16141 gatccagcag cgccgcctcg tcgtccggcg cggcgtgctg caccgcctgc gccagccgcg
    16201 ccagctcgtc ttcgccgctg gccagccagg ccaccgactg gcgcgccgcc ggcaggccca
    16261 tcagcgccgc catgcggtag gtttcgatgt ccagcaggcg ctgcaccagc cgccccaggc
    16321 ggcgcggcgt cacgcggccg ggcgcgtgct cgcccaccag caccagcatg cgcaggctgc
    16381 cgtcggcctg caggcgcatg tcggtgtgca ggtcggagct gtggctgatg acggtggagc
    16441 cggtgacgct gctggcgtcg aacagcgcgc gcacggcggc agcgccgtcg acggcctcgc
    16501 gcggcaccgc ccacaggcgc agccgggtca ggcactgacc gggcaatgcg gccagccagg
    16561 ccgcgggcac gccgtcggca tcgtcgcgcg gtgcgccggc ctgcagtgct tgcaggtcgg
    16621 ccgccgccat cggccgcaaa aaagtccagg cgacgaactc ggtgtgctgt tcccagcgca
    16681 gcgtcagctc gggccactgg gcgcgatgga agttggcgcc ttccggcggc gccggcagac
    16741 cggcggcagc cagcagactg gccagatggg cgcggctctg ctggcgcgca gcggcgtcgg
    16801 cccacatcac gcaatgcagc accgccagcg gcgcgctcag ggcttcgggc gggcgggcgt
    16861 gcagttcgtg gtgcagttcg gctctcaggg ggtgcgcggt gggcatgagg tggtgtgcat
    16921 gggcggatcg ggctgcattg tgtcggagtc cggccggcac cgtcgccgcc aagcaagaac
    16981 ggcacccgcg ggggtgccgt tcttcgttct ggcttggggc ccgcaggcgg gcgggcgtca
    17041 gtcttcgacg aaggcttcct cgcggctctt cttgatcgcc ggcagcagca cgatcaccag
    17101 cagcgccgcc gccagcgcca gcagcgaggc cgacagcggg cgcgtcacca gaatgctcca
    17161 gtcgccgcgc gacagcagca gcgcacggcg caggttttct tccatcatcg ggcccaggat
    17221 caggcccagc agcagcggcg ccggctccat cgacagcttg tggaagatgt agccgacgat
    17281 gccgaaccag cccatcatcc agatgtccca ggtgttgttg ttggtggtgt acacgccgat
    17341 ggcgcagaac agcacgatgg ccgggaacag ccagcggtag ggcaccgtca gcagcttgat
    17401 ccacaggccg atcagcggca ggttcaggat caccagcatg gcgttgccga tccacatgga
    17461 ggcgatcagg ccccagaaca gctcggggtt gctggtcatc acctgcgggc cgggctggat
    17521 gttgtggatg gtcatggcgc ccaccatcag cgccatcacg gcgttgggcg gaatgcccag
    17581 cgtcagcagc gggatgaagg aggtttgcga gccggcgttg ttggccgatt ccggcgccgc
    17641 cacgccgcgg atgttgccct tgccgaacgg catttcgccg gacttgagct tggttttctt
    17701 ctcgatcgtg taggccgcaa acgccgacag cacggcgccg ccacccggca aaatgcccag
    17761 cgccgaaccc agcagcgtgc cgcgcacgat ggacggcgtc atcagcttcc agtcttcctt
    17821 ggacgggtac aggttctgca gcttgacgtc gaacacttcg cgcttgccct gggcatgcga
    17881 caggttggcg atgatctcgc cgtagccgaa cacacccatg gcgatggcga tgaagccgat
    17941 gccgtcggtc agctcgggga tgtcgaagct gtagcgcgcc acgccggagt tgacgtcggt
    18001 gccgaccagg cccagcagca ggcccagcac gatcatgccg acggccttga tcagcgaacc
    18061 ggaagccagc accacggcgc cgatcaggcc cagcaccatg agcgagaagt attcggccgg
    18121 gccgaacttg aaagcgactt cggtcagcgg cacggcaaag ccggccagca ccagggtgcc
    18181 gaagcagccg gcgatgaacg agccgatggc ggccgcagcc agcgccgggc cggcgcggcc
    18241 cttgcgcgcc atctggtggc cgtcgatcac cgtcaccacc gacgaggcct cgcccggcag
    18301 gttgaccagg atggcggtgg tcgagccgcc gtactgcgcg ccgtaataga tgccggccag
    18361 catgatcagc gccgccaccg gcggcagcgc gtaggtggtg ggcagcagca tggcgatggt
    18421 cgccaccggg ccgacgcccg gcagcacgcc gatcagcgtg cccagcaggc agccgatgaa
    18481 ggcgtacatg aggttctgca gcgtgaaggc cacgctgaag ccaagggcaa ggtgttccag
    18541 caattccatg gtgtgtgctc cctgcgactc agcccgaaat gaagctcggc cagaccggca
    18601 tctgcaggtt caggccccag atgaagacca accagctgcc ggcggccagg acgatggcca
    18661 ggatgatcac ttccttgagc ttgaactccg gccccgcctt ggccgccacg agcgtgagca
    18721 ccaggatgcc gatcatcacc ccgagcgacg gaatgcccag cgaaggcacg ccgcccagca
    18781 gcaggccgaa caggaagttg gccaggatga tgtagatcag cggcttccag gcccacgggc
    18841 cgatcgggtc gccgtcgggc gtttccacca ccagcgactt gaccacgatg atggcgccca
    18901 gaatggccag cagaatgccc accagcagcg gaaagtagcc cggccccatg cgggcggcgt
    18961 tgccgatcgt gtagttgttg gcgccgatgg caaaggcgcc gccgatgacc acatacagca
    19021 gcccggcaaa gaagtctctc tgacttttga tgcgcacgaa tcttctcctg gagtgcaaaa
    19081 cccagcgccc attgtcacca gcgcatgggc gccggtcgat gtggattcca cctagaccgc
    19141 ccctgggatt accctagggc gcacgagggg gacggtggca tgcagacgcc cccgatggct
    19201 gcggtggacg cagcatgctg cgccatttcg gcgggcgcgg cggccgatac gcccggcaac
    19261 tcaggagtgg gctcgcggct ccagcggcgg cgtggcggcg atcacctcct gcagcgtggt
    19321 caggccttcg gcggcgcgca gcgcaccggc cagccgcagt gggcgcatgc cgtcggcgat
    19381 cgcctgctgg cgcagcgccg ccaggtcgac atcggcacgc acccggtcct tgaaggcgtc
    19441 gctgacggtg agcagttcgt agatgcccat gcgaccgcga aagcccgtca tgcggcagtc
    19501 cacgcagccc accggcttgt acgggaggta gtcggcggcg ctgatcttcc agggtttgat
    19561 gacatcggcc agcagcggct gcgaaccctg ctcgtccggc tggcggcagt gcgggcacag
    19621 ggtgcgcacc aggcgctgcg ccagcacccc caggatggtg gccgacagca ggtagggcgg
    19681 cacacccagc tccatcaggc gcgtcagcgc cgaaggggca tcgttggtgt gcagggtgct
    19741 gaacaccaga tggccggtga gcgcggcctg caccgccatc tcggcggtct ccaggtcgcg
    19801 gatctcgccg atcatgatga tgtccgggtc ctgccgcatc agggcgcgca cgccttcagc
    19861 gaagctgaag tcgagctgcg gctgcacctg ggtctggttg agcgccgggt cgatgcgctc
    19921 gatcgggtcc tcgatggtgc tgacgttgac ctcctccgtc gccacgcggc gcagggtgga
    19981 gtacagcgtg gtggtcttgc ccgagccggt cggcccggtc accagaatga tgccgtgcgg
    20041 gcgctgcacc agcgcttccc agcgcgccgc gtcgtgcggg gtgaagccca gcgccgccag
    20101 gtccttgacg gcggtgtcgg ggtcgaagat gcgcatcacc agcttttcgc cgaaggcggt
    20161 gggcagggtg gacaggcgca tctcgatttc ttcgccgccc gggctgcgcg tcttgatgcg
    20221 tccgtcctgc ggccggcggc gctcgaccac gtccatgcgg cccagcagct tgatgcgcgc
    20281 ggtgatggcg ttcatcacgc cggcgggcac ctgatagacc gggtgcagca cgccgtcgat
    20341 gcgaaagcgg atcacgccct gctcgcgccg cggctccaga tggatgtcgc tggcgcgctg
    20401 atcgaaggcg tattgccaca gccagtccac cacctggatc acgccctggt cgttggcgtc
    20461 gagctggcga ccggtgcgac ccagctcgac cagctgctcg aagctggcgg cgccggcgct
    20521 gccgccaccc tgcttctgcg ccacccgcac cgaccgggcg agggcaaaga actcggccgt
    20581 gtagcgcttg atgtcgagcg ggttggccac cacgcgccgc acctgccggc gcgattggcg
    20641 ctcgacctcg gccacccagt cgtcgatgaa gggctcgctg gtggccacca ccacttcggc
    20701 cggcgtgacc tgcaccggca gcaccttgtg gcgttcggcg tagctggcgc tcatggtgtc
    20761 ggacaccttg cccacgtcca cccgcagcgg gtcgatgcgc aagtaggcca ggccggcgcg
    20821 ttcggccagg tagcgcgtca gcgcttccac gtccatggcg gcgccgtcgg cagcgcgcgt
    20881 gatgcccacc gccgccagcc gcaccagggc gtgctgcgca ctctcggcct gcgcgcaacg
    20941 cgcatgcacg cgggcggctt cggcggcgct gatgaccgcg tcctcgcgca gccattgcag
    21001 cagcaggcgc cagtgcaccg ggccacggta gccgtcgacg acggtcaggg gggtagggcg
    21061 ggtggaacgc ttcatttctc gatcgccttg aaaaccttca gccatttgcg cgccggcagg
    21121 ccccagcgtg cctcgatctc ttcggcgcgc tgctgcagca gcggacgctt ggggcgcgtt
    21181 gcgctgcgct gggccagcac cacggtgttg ccctcgcgcg tggccttgaa ggcccacacc
    21241 gcctgggcgc cgaaggcttc gctgatgcat gccaggctgc gttcgaagct gctggagcgg
    21301 ccgaacaggt tgactgtcat gcagccgtct tcggtcagca ggcgccggca gtcggcatag
    21361 aaagcggccg aatccagcac cggcgcggcg gcctcgtcgt cgtacagatc gaccgccagc
    21421 gcgtcgaccg tgcccagcca ctcgtcgcgg cggatttctt cggcggcgtc ggcgagcacc
    21481 acgcgcaggc gctcgtcgtc gggcggcagg cgcagccagg cgcggcagac gtcggccacg
    21541 cgcgggttca gctccaccgc ggtgcaggtc atgcgcagct tcttgtagca gaacttggtg
    21601 atcgcccctg cccccagacc cagctgcatg gcatggcgcc cggccacgct gtcttccggc
    21661 acgaacagca gccaggccat catgcgctgc acgtattcca ggtcgatctc gaacggcgcg
    21721 tcgatgcgca tcgacccctg cacccattcg gtgcccaggt gcaggtatcg cacgccgtcg
    21781 tgttcggaca ggttgacttc gctgaaggtg ggctgctggc gtttcaagat ggacggaggt
    21841 tatcaccgca ggtgcccgcg ccggggcggt ttgcccgccc gcaggccgcg cacacggtct
    21901 gcgggcaccc tgcgacgtga catgcatcac ggcgcagacc gtgggcgccg gcggcgcggg
    21961 cgaagtcgtg gcaaagtcgc cgcctcggac attcacccgc gcattgcgag caaccaccat
    22021 gaacacccag gacatcctgc atttcatcac cacccagggc gccgagttcg gcctcaagct
    22081 gctgaccgcc atcgccgcct ggatcgtcgg ccgctggttg atcgggctgg cggtcggcct
    22141 gatgggcaag atcttcgagc gcggcggcaa gatcgacacc acgctggccc actacctgaa
    22201 atccatcacc agcgtgctgc tcaacatcgt cctgatcctg gcgatcctgg acatcttcgg
    22261 cgtcaagacc acctcgtttg ccgcgctgct ggccggcgcg ggcctggcca ttggcgcggc
    22321 ctggagcggc atgctgggca actttgccgc gggcgtgttc atgcaggtgc tgcgccccta
    22381 caaggtgggc gacttcatca gcgccggcgg cgtggtgggc acggtgaccg agctcggcct
    22441 gttcgccacc acgctgacca cgcccgacaa cgtgatgacc gtggtcggca acggcaagat
    22501 ctttggcgac aacatccaga acttcagcgc cctgccggtg cgccgcgtgg actgcgtggc
    22561 caaggtggcc aacagcgtga acccgctgga cgcgatggcg cgcctcaagc ccgtgatcgc
    22621 cgcgattccc aacgtggccg cctcgcccgc gccggacatc gagatcctgg aattcacgcc
    22681 cgaaggcccc aagctctgcg tgcgccccta cacccacacg gaccattact ggcaggtgta
    22741 cttcgacacc cacaaggcca tcgtcgagac ctttggcgcc gccggctatc cggttccgga
    22801 gacgccggtg gcctatcgca gcgcgccttc cggggtggcg tgacggccgg cgtgcagaac
    22861 aacccaaccg ccggaagcag aagtcgccaa ggattcgcgc aggacgcgaa agaggtcgaa
    22921 gaagggcccg tcatccccgc tgtcgcgggg atgacgcatc cgttgcgtca gcccgccgcc
    22981 gccgccagcc gctcggcgca gcgctgggcc tgggcggcat ccaccgcctc gaccatcacg
    23041 cgcagcagcg gctcggtgcc gctggcgcgg atcagcaccc ggccgctcgc gcccagttcg
    23101 gcttcgacgg cgcgcgtggc gtcggtcagg cgggtgtagt ggcgccagtc ctgaccggcc
    23161 tgcaggcgca cgttgatcag cacctgcggg cacagcgcca caccctcgag cagctgcgcc
    23221 agcgtgcgcc cctgcatggc gcaggcctgc agcacctgca gggcggagat caagccgtcg
    23281 ccggtggtgt gcttgtccag cgccagcagg tggcccgaac cttcgccgcc cagaatccag
    23341 ccgcgctgca ccagctcttc cagcacgtag cggtcgccca ccttggcgcg cacgaagtcg
    23401 aaaccacgcg cgcgcagcgc ctgctcgacc gccaggttgg tcatcagggt gccgaccacg
    23461 cctgccagcg ccggcgggcg cggcgcgccc gcgccgcgca ggctggcgtc gcgcgccagc
    23521 cggtcgatcg ccagcacgta cagcagttcg tcgccgttga acaggcggcc gctggcgtcg
    23581 accatctgca ggcggtcggc gtcgccgtcc agcgcaatgc cgtagtcggc gccgtgttcg
    23641 gccacggcgc gcaccagcgc ctcggggtgg gtggcgccga cggcgtcgtt gatgttcagg
    23701 ccgtcgggcg cgcagccgat ggccagcacg tcggcgccca gttcatggaa caccttgggc
    23761 gcgatctggt aggcggcgcc atgcgccgca tcgaccacga tcttcagtcc gcgcagcgtc
    23821 aggtcgttgg caaaagtgct cttgcagaac tcgatgtagc ggccggcggc gtcgtcgagc
    23881 cggcgcgcgc gccccagggc atcggcactg acccactgcg gcacctcggc cagcacggcc
    23941 tcgacttcgc gctcccagtc gtcgggcagc ttggtgccgc gggcgctgaa gaacttgatg
    24001 ccgttgtcgg ggtagggatt gtggctggcg ctgatcacca cccccagctc ggcgcgctgg
    24061 gcacgcgtca ggtaggccac ggccggcgtg ggtaccggcc cgagcagcac cacgtcgacg
    24121 ccggccgagt tgaagcccga ttccagtgcg ctctccagca tgtagcccga aatgcgcgtg
    24181 tccttgccga tcagcaccgt ggcgtcgggc tgcgtgcgtc gcagcacgcg cccgaccgcg
    24241 tggcccaggc gcagcgcgaa atccggcgtg atcggggcct gccccacggt gccacgaatg
    24301 ccatcggtgc caaagtactg tcggctcatg cccaagctcc ctccttagaa agaaaaacgg
    24361 cgcttgcgcc cgcagggcaa gcgccagcag ctatgatttt aatagtgtct ggcgggcctg
    24421 gcgccacacc gccagcgcct gcacggtttc gcgcacatca tgcacgcgca cgatgcgggc
    24481 gccgcgctcg accgccagca gcgccgccgc cacactgccc agcacgcgct cggcaggcgg
    24541 tggcggcgcc ggcgcatcgc cctgcagtgc tgcgccgatg ctggacttgc gcgaccagcc
    24601 gaccagcagc ggccagcccg ccgccagcag ctcgcgctgg cgcgccagca ggctgaagtt
    24661 ctgcgccacg gtcttgccga agccgatgcc cgggtccacc acgatgcggg tcctgtcgac
    24721 gcccagcgct tgcaggccct gcgccgcatg ctccagaaac gagagcacct ggggcggcgc
    24781 gtcgccctcc atgggggcgg tctgcatgct gcgcggctcg ccatgcatgt gcatcagcac
    24841 gatgccgcag gacgggtggc gcgccacgag ctgggccgcg ccgggctggc gcagcgccca
    24901 gacgtcgttg acgatgtcca cgcccagatc cagcgcggcc tgcatgacct cgggtttgta
    24961 ggtgtcgatc gacaccggca cgcccagctg cacggcaccg cgcaacacgg gcagcacgcg
    25021 cgccagctct tcggccagcg gcagcggcgg gctgccgggg cgggtcgatt cgccgccgat
    25081 gtccagcagc tgcgccccgt ccttcagcag gcgctcgcag tggcgcaggg ccgaggcggc
    25141 gtccgggtgc tggccgccgt cggcaaacga gtcgggcgtg acgttgacga tgcccatcac
    25201 ccgcggctcg gccaggtcga tgcgaaagcg gctggtctgc cagatcatgc gacgtcaata
    25261 gaaacggggc cgcggccccg tagaaaccag aaaatcctga acctccctac gcggaggatg
    25321 caaagaattc gcagagaacg cagaaaaacc tggatgggcg gttcttttgc gtctttggcg
    25381 caagctttgc gtccttgacg tacgggggtt cgacgggttc gtcagcggcc caatcaggct
    25441 gctgtcggtg ccggatcggc cggcttggct tcggccgggg tgccgccggt gccgcccgag
    25501 ggcggcgtgc gtggcgtgaa gtccttgggc ggacgcggct cgcgcccggc catgatgtcg
    25561 tcgagctgct cgccgtcgat ggtttcccat tccagcagcg ccttggccat ggcgtgcatc
    25621 ttgtcctggt tgtcctcgat cagcttgcgc gccagcgcgt actgctcgtc gatgatgcga
    25681 cgcacctcgg catcgacctt ctgcatggtc tcttccgaga tgttggtggt cttggtgacg
    25741 ctgcggccca ggaacacctc gccctcgttt tcggcataca ccatgggccc catggcctcg
    25801 ctcatgccgt aacgcatcac catgtcgcgc gcgatctggg tggcgcgctc gaagtcgttg
    25861 ctggcgccgg tggtcatctg gttcatgaac acctcttccg cgatgcggcc accgaacagc
    25921 atgctgatct ggttcagcat gtactgcttg tcgtagctgt agcggtcgtt ctcgggcagg
    25981 ctcatggtca cgcccagcgc ccggccgcgc gggatgatgg tgaccttgtg caccgggtcg
    26041 cacttgggca gcagcttgcc gatcagggcg tggccgctct cgtggtaggc ggtgttgcgg
    26101 cgctcctctt cgggcatgac catgctcttg cgctcggggc ccatcaggat cttgtccttg
    26161 gccttttcga agtcctgcat ctcgaccacg cgcgcgttgc ggcgcgcggc catcagcgcg
    26221 gcctcgttgc acaggttggc cagatcggca ccgctcatgc cgggcgtgcc gcgcgcgatg
    26281 atggcggggc tgacgtcctg gccgatcggg atcttgcgca tgtgcacgtt caggatctgc
    26341 tcgcggccgc ggatgtcggg cagcgtcacg tagacctggc ggtcaaagcg gccggggcgc
    26401 agcagggcgg cgtccaggat gtcggggcgg ttggtggcgg ccaccacgat cacgcccagg
    26461 ttggtctcga agccgtccat ctcgaccagc atctggttca gggtctgctc gcgctcgtcg
    26521 ttgccaccgc ccagtccggc gccgcgctgg cggccgacgg cgtcgatttc atcgatgaag
    26581 atgatgcagg gcgcgttctt cttggcgttc tcgaacatgt cgcgcacgcg
//
LOCUS       DAOQTZ010000029        26267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2171_length_26267_cov_14.589615, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000029 DAOQTZ010000000
VERSION     DAOQTZ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 26267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26267
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_26267_cov_14.589615"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..1681)
                     /gene="hslU"
                     /locus_tag="PKN51_05230"
     CDS             complement(344..1681)
                     /gene="hslU"
                     /locus_tag="PKN51_05230"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNT84535.1"
                     /translation="MSSMTPQEIVSELDRHIVGQHDAKRAVAIALRNRWRRQQVDEKL
                     RQEITPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGKDVDAIIRD
                     LAEMAVKQQREQAMKAHRTRAEDLAEDRILDVLLPPARQADGSNAPAGDSAARQAFRK
                     KLREGQLDDKEIELELAQPAPTMEILGPPGMEEMAEQLKGMLGNLGGGRRKLRRVRIG
                     EAMKLLVDEEAGKLVNEDDIRTEAVRNLEQNGIVFIDEIDKVASRSLEGGGSGAEVSR
                     QGVQRDLLPLVEGTTVSTKYGMVRTDHILFIASGAFHLSKPSDLIPELQGRLPIRVEL
                     ASLSVQDFEAILTQTDASLVRQYQALLATEGVTLDFRPEGISRLAEIACAVNEATENI
                     GARRLSTVMERLLEEVSFDASRRQGQTLTIDAAFVDARLAVLSQDEDLSRYIL"
     gene            complement(1769..2314)
                     /gene="hslV"
                     /locus_tag="PKN51_05235"
     CDS             complement(1769..2314)
                     /gene="hslV"
                     /locus_tag="PKN51_05235"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNT84536.1"
                     /translation="MEAFHGTTIVSVRRQTPQGWQVAIGGDGQVTLGHIVVKGTARKV
                     RKLHHGRVLAGFAGATADAFTLFERFEAKLEKHQGHLTRAAIELTKDWRTDRVLRRLE
                     AMLAVADREASLIITGNGDVLEPEHGLVAIGSGGAYAQAAARALLAHTDLPAEQIVRK
                     SLEIAGELCIYTNMQHTIEVL"
     gene            complement(2379..4115)
                     /locus_tag="PKN51_05240"
     CDS             complement(2379..4115)
                     /locus_tag="PKN51_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNT84537.1"
                     /translation="MNKPTEPGGLLSKVVRFVKNPTTHWADLDQPDSEDGDSQSRLAL
                     KEMIERKRRNDFVRSREFDMLRKLRRRETLKGSDLAAGPPSFYPSSHPANTGERELTL
                     KKIDEIEAQMSTAWSRHRVGKNQAAGPAADAISEPSVHAYDKTDPMPLPPPPEGGAPL
                     LDAAADGSASALAKESLSAQPLHPSAGGAAAARAIPWLGEVQDFNVEVMAAGRQDPEI
                     EEAAILFANGDGAGAEALLRALVAEGGARREDVDTWLTLFDLYRCIGDQARFDDAAPE
                     FAAQFGRSAPQWTGMLDRPSSGVSPLTAAPAPGATAAGPFHWISPSALGTQSMATLRM
                     SLERHAPPWRLDWRHLQSIEAAALPSLVEQLQRWGRTPVRLKLLGIERLMDVLTERSP
                     TGDRTVDPQWWEARLALLRVLGQMDEFDLVALNYCVTYEVSPPAWEDPVNGWTRMNEE
                     GETLMSSRFGSDTLGVRMPEPGGDEAPSHDTEEPPEGVVRTVLDGAYSGSMSALLQPL
                     AQRGEVRHFEFNCRQLVRVDFGAAGDLLNWATEQQGSGRSISFKQVNRLVAAFFGVIG
                     ITDVSRVLLRTD"
     gene            complement(4320..5015)
                     /gene="dksA"
                     /locus_tag="PKN51_05245"
     CDS             complement(4320..5015)
                     /gene="dksA"
                     /locus_tag="PKN51_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNT84538.1"
                     /translation="MKTAAAKSPATPKAKPAKAPSSAGKPASKARVSAPEPSKKSPVR
                     SAAAKASTAVPTKPPQPVSRNLPMPSVATPPMPAPVKKDPKLANNWKTKSVEQLSDPE
                     IIAMPDNEYMNDKQLAYFRLKLVQLKEDMLANADQTAENLRDDTVVVPDPADRATIEE
                     EHALELRTRDRERKLLKKIEQSIARIDAGDYGYCDETGEPIGVPRLLARPTATLSLEA
                     QQRRELKQKMFGD"
     gene            complement(5440..6501)
                     /locus_tag="PKN51_05250"
     CDS             complement(5440..6501)
                     /locus_tag="PKN51_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNT84539.1"
                     /translation="MSLIPATILTGFLGSGKTTLLKRVLTEAHGQKIAVIENEFGEEN
                     IDNDILVSDSQEQIIQMSNGCICCSIREDLRATLSDLARRKRSGELDFERVVIETTGL
                     ADPGPVAQTFFMDDEIAEGYLLDAIVTLVDAKHAAQQLNDRIEAQRQVGFADQIFISK
                     ADLVDAGELAALQHRLKHMNPRAAQKVVHFGAVPIAEVFDLRGFNLNARLDIDPEFLA
                     ADDHDHDHHDHHHHHDGHCDHPSHQHAHGHHHHADDDVKSFVFRAERAFDPARLEDFL
                     GAIVNVYGPRMLRYKGVLWMKGTERKVIFQGVHQLMGSDLGPAWKGDEPRQSKMVFIG
                     IDLPRDIFTQGLEQCLVSA"
     gene            6803..7618
                     /locus_tag="PKN51_05255"
     CDS             6803..7618
                     /locus_tag="PKN51_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNT84540.1"
                     /translation="MSKSFNLVALALSLGITGAQARELVLGISEGTSGGTDHARVVQK
                     YGGLAEAIGKAIKAEVKVVFVREFAQLEDGMKSARLDLVMARPSDYPARGLRDYGYHY
                     VATVKPEGQCLIVVPKDSPIKQLADLKGKKIVMPEKVAYMTKFCKAELRHQGVDLDKE
                     SVTYVREQEAVVFYINNGFGQVGGLASYSGAARKWLKEGGTVLHRSVPQPYSPLIASK
                     ALSAADIAAVQKAVLALPDSPEGQVVLKSIGMPGGFDTATEDKLRGLLKFLGD"
     gene            complement(7643..8617)
                     /locus_tag="PKN51_05260"
     CDS             complement(7643..8617)
                     /locus_tag="PKN51_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNT84541.1"
                     /translation="MSSVVEPLPSAAAADAALVERYLTHARVLKRLAPRTLALYRADL
                     DKLARLAAQAGVALPQVQHHHVRSWVARMHAGGRSGRGIALILSGWRGFYVWLGREGG
                     IGANPVLGIRAPRQPRPLPKALGVDEAVQLAEHIDEGDDPWLDARDAAMVELLYGSGL
                     RVGELVGLDVAASDAAARAGRGWIDLDQAEVQVQGKGSKRRAVPLGRSAAEALRRWLA
                     VRGQVMAAGEAAAALFIGRRGTRLSAPSVWQRLRRRSQQAGLATPVHPHMLRHSFASH
                     VLQSSGDLRAVQELLGHASIGTTQVYTRLDFQHLAQAYDAAHPRARRR"
     gene            8983..9456
                     /locus_tag="PKN51_05265"
     CDS             8983..9456
                     /locus_tag="PKN51_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNT84542.1"
                     /translation="MAAHPAHALALGFGSGLSPVAPGTAGTLWAWLAWWALGLWLSPA
                     QQGWLLVGALPLAWWACTVTAQHLQTPDPGAIVIDEVVAFWLVLWLLLPAGFWGQATA
                     FALFRFFDAVKPGPVGWADRLLHGRRGWAGGFGVLLDDLVAAFCTLLVLALWRPG"
     gene            9463..10008
                     /locus_tag="PKN51_05270"
     CDS             9463..10008
                     /locus_tag="PKN51_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNT84543.1"
                     /translation="MLPKQELSALAGSAPEAKIVHDIAALLTARGWLLTTAESCTGGL
                     VAAACTELAGSSLWFERGIVSYSNAAKSELLGVPPALIARHGAVSEPVVRAMAEGALA
                     HARAQVSVALTGIAGPTGGSADKPVGTVWFGWCVDGRTDTQRCRFDGDRQAVRAQAAL
                     HALRGLAARLAAPGGAATPLT"
     gene            complement(10001..11584)
                     /locus_tag="PKN51_05275"
     CDS             complement(10001..11584)
                     /locus_tag="PKN51_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT84544.1"
                     /translation="MKLRTLAVAALAVCCTLGAHARTFKWTSASDIPTLDIHSQNNAL
                     GNGVHAAVYESLVYYNSKSFKIEPMLATAWKQVSPTQMQFTLRTGVKFSDGSSMTAED
                     VKFSIERAMAKTSNYSVYTQGIDKVVVAGPNTLDIFTKAPNPVLLNQLTELRVMSKAW
                     AEKNKSVEPKDIKTKDESFAHRNAMGTGPFMVKEWQPDQKLVLVDNPQWWGHGAKAKP
                     GNVTEVIYTPIKSEATRMAALLSGEVDFVLDPTPQDLERVQRNAALKVVQGPENRTIF
                     FGMDQHRDELPGSSVKGKNPLKDQRVRQALYQAIDINAIHKVVMRGLSQNTGTLIAPQ
                     VSGYTKRADQRYPYDAAAAQKLLAEAGYKDGFEVDFACPNNRYIADERICQAVAAMWS
                     KVGVKAKLRTMPLVSYFPMIQRHEASIYMLGWGVPTFDALYSLQSLVRSVGAGGDGNY
                     NVGRYSNPQMDALVERVKVEVDQKRRAELIEQALILEHKDVSHLPLHNQVIPWAMKKN
                     IEVVHRADNRLDWRLIAVK"
     gene            11794..12570
                     /locus_tag="PKN51_05280"
     CDS             11794..12570
                     /locus_tag="PKN51_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT84545.1"
                     /translation="MSSDIILETRGLVKEFKGFVAVNDVNLQVRKGTIHALIGPNGAG
                     KTTCFNLLTKFLEPTRGQILFDGQDITAERPAQIARRGVVRSFQISSTFPHMSVLENV
                     RVSLQQKLGTSFHFWKSERTLDQLNERCMELLGFVGLADFAHLEAVELPYGRKRALEI
                     ATTLAMDPKLMLLDEPTQGMGHEDVDMVTQLIKKVAANRTVLMVEHNMSVVGSIADQI
                     TVLQFGQVIAEGPYAEVSRNPQVLEAYMGSAEEALQGAHG"
     gene            12584..13297
                     /locus_tag="PKN51_05285"
     CDS             12584..13297
                     /locus_tag="PKN51_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT84546.1"
                     /translation="MSHTSAPAIVLKDVHSWYGESHIMHGVHLEVKPGEVVTLLGRNG
                     AGRTTTLRAIMGLVGKRTGSIQVNGVETVKLAPHHIARLGLGYCPEERGIMTSLTCEE
                     NLMLPPKVAEGGMSVDEIYDMFPNLKARRNSPGGRLSGGEQQMLAVARILRTGAKVLL
                     LDEISEGLAPVIVQALARMIRELRARGFTVLMVEQNFRFAAPLADRFYIMERGLVLRE
                     FSAAELQDNMDMLHQYLGV"
     gene            13377..14594
                     /locus_tag="PKN51_05290"
     CDS             13377..14594
                     /locus_tag="PKN51_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT84547.1"
                     /translation="MKRKTLCALITGLGAAFGGAAYAQISGGVVRIGVMNDMAGPYAD
                     LAGPGSVVAARMAVDDYLKATNSKLKVEIISADHQNKPDVGSSIARKWYDSDGVDMIL
                     DVPTSSVALAVNEVTKEKNKVMVNSTGGTADLTGKACTPNTVHWTYDTWMLAHGTGSA
                     VVKQGGDSWFFLTADYAFGHALERDTSDVVKASGGKVLGSVKVPLGTADFSSFLLQAQ
                     SSKAKIIGLANAGSDTINSIKQAAEFGIVKGGQKMAGLLVFLSDIHGLGLNTAQGLQV
                     TTTFYWDLNDATRAWSKRFGAAHQNRMPTMVQAGVYAGLQHYLKAVEALQSDSDGAKV
                     VAKMKELPTDDPLFGKGTIRVDGRKIHPAYLVEVKKPSESKYAYDYYKVLQTIAADKA
                     FRPLNEGNCPLVK"
     gene            14637..15857
                     /locus_tag="PKN51_05295"
     CDS             14637..15857
                     /locus_tag="PKN51_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT84548.1"
                     /translation="MKRNALWSLLAGLGLLAAGSAHAQISGNVVKIGVMNDMSGLYAD
                     IGGPGSVIAAKMAVEDYLKATKSSLKVEVVSADHQNKPDVGSSIARKWYETDGVDMIA
                     DVPTSSVALAVNQVTKDMGKAFVNTGAATSDLTGKDCSPNTVHWLYDTWMLANGTGSA
                     VVKAGGDSWFFLTADYAFGHALERDTSEVVKASGGKVLGSVKVPLGTADFSSFLLQAQ
                     SSKAKIVGLANAGGDTINSIKQAAEFGIVKGGQKLAGLLVFLPDVHGLGLNTAQGLNI
                     TEAFYWDLNDGTRAWSKRFAAAHGGKHPSSDHAGVYAGVLHYLKAVDAAKTDDGSKVV
                     AKMKELPTDDPLFGKGTVRADGRKIHPVYLFEVKKPSESKAPYDYYKLVQTIAADKAF
                     RPLNEGNCPLVAKK"
     gene            15887..16771
                     /locus_tag="PKN51_05300"
     CDS             15887..16771
                     /locus_tag="PKN51_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT84549.1"
                     /translation="MEIFGIPIQAMMGQLLIGLINGSFYALLSLGLAVIFGLLNIINF
                     AHGAQYMLGAFGAYLLLNKLGLGYWWSLLVVPILVGATGVVIERTMLKPLYKLDHLYG
                     LLLTFGLALIIQGLFRNEYGSTGLPYAIPEALQGGKNLGFMFLPNYRAWVIVASLVTC
                     LSTWFVIEKTRLGGYLRAATENPQLVQAFGINVPRMITLTYGFGVALAALAGVMAAPI
                     YQVSPQMGENLIIVVFAVVVIGGMGSIMGAIVTGFGLGLIEGLTKVFYPEASTTVIFV
                     IMTIVLLIRPAGLFGAQK"
     gene            16878..17771
                     /locus_tag="PKN51_05305"
     CDS             16878..17771
                     /locus_tag="PKN51_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT84550.1"
                     /translation="MALIFIGAPLVGVYPVFMMKVMCFALFACAFNLLLGYGGLLSFG
                     HAMFLGSAGYVSAHAAKVWGLTPELAIALGASCAGALGWVAGKLAIRRQGIYFAMITL
                     ALAQLVFFFSLQAPFTGGEDGIQAVPRGKLLGLIDLANPAAMYPFVLAIFLGGFLLIY
                     RIVHSPFGQVLKAIRENEARAISLGYDADRFKHRAFVLSATLAGLAGATKAIVFQLAS
                     LTDVHWSMSGEVVLMTLVGGMGTLFGPVVGAAVIVSMQNYLAQLGAWVTVVQGAIFVL
                     CVLLFRRGIIGEIGHLLKKSL"
     gene            complement(17873..19450)
                     /locus_tag="PKN51_05310"
     CDS             complement(17873..19450)
                     /locus_tag="PKN51_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT84551.1"
                     /translation="MPAGSGFKLTYSTMFNPPPELHARFDAALASFRRDGMGRDHAQW
                     IGGMSVGGARHFEVRSPIDQDWLIGRFVEGSAQDVDRAVQAAQAAYPGWSATPWRERV
                     ALLRRAARLIEERVYAISAAVALEVGKNRMESIGEVQETADLIDWYCDQMEAHDGFDR
                     ALPDDPLPQFRSHNRTVLRPYGVWAVIAPFNFPFALAGGPLGPALVAGNTAVFKVSPE
                     TSLSGRLLLDALHDAGLPAGVINFVTGGDATGRALVEHPGVAGITFTGSQAVGMQVLR
                     QSVQGPFPRPCIVEMGGKNATIVSRRADLERAALGIVRSAFGLSGQKCSACSRVYVEQ
                     PVADDLRARLVRLAGELSVGDPTQQAHWMGPVIGRAAYERYAGLAEHLRQVGRIDTGG
                     RWLTEGALARGFFVAPTVAQAPLDDRAWVDEHFLPLVLVGEVASVAEGIARANASDYG
                     LTAGFYGAPDEVAYFHRHIEAGVTYANRPQGATTGAWPGYQPFGGWKGSGSTGKAIGS
                     FYYLPQYLREQSRTVVE"
     gene            complement(19550..20737)
                     /locus_tag="PKN51_05315"
     CDS             complement(19550..20737)
                     /locus_tag="PKN51_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT84552.1"
                     /translation="MTADERRASLSLAGIFALRMLGLFLVLPVFALEAARYPGGDDPA
                     LVGLAMGIYGLTQAVMQVPLGLASDRIGRKKVIVAGLLVFALGSALAALAGSVHMLVA
                     GRALQGAGAVSAAVTALLADLTRDEVRTKAMALVGISIAAMFALSLMAAPPLTGAIGL
                     SGLFWLTAALALAGVVLVLVATPAEPVQHVDAPRGRLQEVWRHPHLWRLDLGVFVLHA
                     VQMAMWVVVPGLLVQAGLLKAEHWKLYLPAVLVSFVFMGGLFALERRGHLRAVLRGGI
                     ALLLLVQLALLLLASRAPGLWTLGVVMFFFFMAFNLIEASQPSLISRLAPAQARGAAL
                     GVYNTLQSLGLFAGGALGGWLLKGWGPAAVLSGTSALVALWLLLGWGQHVPALRPPAD
                     PPR"
     gene            20870..21373
                     /locus_tag="PKN51_05320"
     CDS             20870..21373
                     /locus_tag="PKN51_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ecotin family protein"
                     /protein_id="HNT84553.1"
                     /translation="MKATHHLPRATAVLALACTLLAACAQQPAAPNPSAAQELKAFPA
                     TLKGHQRHVIELPALPDEADRQIELIGTKAMTVDCNTRGMDGRFESRDVQGWGYNYWV
                     LQSRGQVMGTMMACPPGSQRPGVAQTQGTLLRYNSKLPVVVFVPEGMGLRWRVWSAGA
                     MQDASAR"
     gene            21662..22585
                     /locus_tag="PKN51_05325"
     CDS             21662..22585
                     /locus_tag="PKN51_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84554.1"
                     /translation="MSFLQSTSNTAFFIALGIVVLLSGIGFGIVAWVGRLTGALRGRE
                     PVPINMLTEGYLLARGKASGRTLRAPLSGRPCVWWEMKVWERRIEIDRQASSDEPDRR
                     ASWTLLRHDVSRRPIHCTQGFVTCTVQPDGMTLTVPSEVRDWKGKQYPPENRDPPVQP
                     GSAFSYRQVHDTGFTIVGGKLLGDRYRYVELLIASNAELFVLGRVERVAARAGAASTH
                     DDAPAPEGESVAPFEQAQREENEDEQMSPSMREVQWRIAPEKGRPYIVATQPPEQWMA
                     TIKLASQGGLIVGTVTAALAAFMLWARYQGG"
     gene            complement(22649..23161)
                     /locus_tag="PKN51_05330"
     CDS             complement(22649..23161)
                     /locus_tag="PKN51_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84555.1"
                     /translation="MRSMHCGSLRSATRRAAIGLALVTLVAAGAQAQQASGELALQAR
                     SGVVKVAPRYAYLLAGPSFDGKPMRRLVLSEVDLTSAIQKCDRLGCVSSDLHSGVTVD
                     FDGGPRLGYWFVGKGQMVQHSDTAQPATMQLKQDSHQRLAGSWTLKGDVGATGSVTFD
                     APLAKTFARN"
     gene            23424..>26267
                     /gene="uvrA"
                     /locus_tag="PKN51_05335"
     CDS             23424..>26267
                     /gene="uvrA"
                     /locus_tag="PKN51_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT84556.1"
                     /translation="MNDLAAPDLSDAEGLPLATALRQPRISVRGARTHNLKNIDIDIP
                     KDRLVVITGLSGSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQLMDKPDVDLIEGLS
                     PAIAIEQKATSHNPRSTVGTVTEIHDYLRLLFARAGTPHCPEHDLPLTVQTVSQMVDA
                     TLALPQDTRVMVLAPLARARKGEFSELLAQMQALGYVRFRIDGQVVEADRLPVLQKND
                     KHDIDVVIDRLKVRGTADGPGGAATALAARLADSFEAALRLADGRAIALEMDEGKEHL
                     FNSKFACPVCGHSLPELEPRLFSFNAPAGACPTCDGLGTTDVFDPERVVAFPSLSLAS
                     GAIKGWDRRNGYYFALIESLAKHYRFSVETPFEELPEAVRHVILHGSGDEALRFSYTF
                     DSGAQAGRKVSKKHPFEGILPNMARRWRETDSNVVREELARYRSQQPCTACGGTRLRQ
                     EARHVFVGEGAQRRAIYQISHVTLGEAQAYFDALRLTGAKADIAERVVREIASRLKFL
                     NDVGLGYLSLERSADTLSGGESQRIRLASQIGSGLSGVMYVLDEPSIGLHQRDNDRLI
                     ATLQHLRDLGNTVLVVEHDEDMMRAADHIVDLGPGAGVHGGRVMAQGTFAQVAATPAS
                     VTGQFLRGERAIAVPSRRRRSHIDSCSRTPDGRQSPETLETHRPTPSGVLRIVGARGH
                     NLKDVTVDVPVGLLTCVTGVSGSGKSTLVNDTLYTAAAREIHRAHAEPAPHEAIEGLA
                     QFDKVIAVDQSPIGRTPRSNPATYTGLFTPIRELMAETAMARERGWGPGRFSFNVPQA
                     SGGGRCEACQGDGVVKVEMHFLPDVYVPCDVCAGQRYNRETLQVQWKGRNIAQVLDMT
                     VEAAHAFFADQPAIARKLQTLLDVGLSYITLGQSATTLSGGEAQRVKLALELSKRDTG
                     RTLYVLDEPTTGLHFADVELLLKVLHQLVEAGNT"
BASE COUNT         4280 a         9065 c         8680 g         4242 t
ORIGIN      
        1 ctcgctcaac cggaaaagcc ttgaatgccg gaagtcgcgc cacgggcgcc gggcgtgatc
       61 gcccctgcag gacgcgggcc ggcatgcgcc attaccccct cctgtcaccc ggggaacggc
      121 agttggacgc gtccgcccgt gccgggatcg gcgtgccagg caagacgcga cgacgctgcg
      181 ggctcctgcc cgcaaggaga agcaacgccg catggcgcgg cgagcgcggt gcgggtgggt
      241 gcgcagcgcg ttcactcagg aggtgcaggc catcgcagcc gcgagacccc gcatcgatgc
      301 ggtctgcccg aggttggcaa gcggtcaact gccgtttcta ggatcagagg atgtagcgcg
      361 acagatcttc gtcctggctc agcaccgcca ggcgcgcatc gacgaaggcg gcatcgatgg
      421 tgagcgtctg cccctggcgc cggctggcgt cgaagctgac ttcttcgagc agccgctcca
      481 tgacggtgga caggcggcgc gcaccgatgt tttcggtggc ttcgttgacg gcgcaggcga
      541 tctcggccag gcgcgagatg ccctcggggc ggaagtcgag cgtcacgccc tcggtggcca
      601 gcagggcctg gtactggcgc accaggctgg cgtcggtttg cgtcaggatg gcctcgaagt
      661 cctgcaccga cagcgaggcc agctccacgc ggatcggcag ccgcccctgc agctcgggga
      721 tcaggtcgct cggcttggac aggtgaaagg cgccggaggc gatgaacagg atgtggtcgg
      781 tgcgcaccat gccgtacttg gtgctcacgg tggtgccctc gaccagcggc agcaggtcgc
      841 gctgcacgcc ctggcgtgag acctcggcgc cactgccgcc gccttccagg ctgcgcgagg
      901 ccaccttgtc gatctcgtcg atgaagacga tgccgttctg ctccagattg cgcaccgcct
      961 cggtgcggat gtcgtcctcg ttgaccagct tgccggcttc ttcgtccacc agcagcttca
     1021 tggcttcgcc gatgcgcacg cggcgcagct tgcgccggcc accgcccagg ttgcccagca
     1081 tgcccttgag ctgctcggcc atttcttcca tgccgggcgg ccccaggatc tccatggtgg
     1141 gcgcgggctg ggccagttcc agctcgattt ccttgtcgtc cagctgacct tcgcgcagct
     1201 tcttgcgaaa ggcctgacgc gcggcgctgt cgccggccgg cgcgttgctg ccgtcggcct
     1261 gacgcgcggg cggcagcagc acgtcgagga tgcggtcttc ggccagatcc tcggcgcgcg
     1321 tgcggtgcgc cttcatggcc tgctcgcgct gctgcttgac ggccatctcg gccaggtcgc
     1381 ggatgatcgc atcgacgtcc ttgccgacgt agccgacctc ggtgaacttg gtggcttcga
     1441 ccttgatgaa gggggcgttg gcaagccggg ccaggcgtcg cgcgatctcg gtcttgccga
     1501 cgccggtggg accgatcatc aggatgttct tgggcgtgat ctcctggcgc agcttctcgt
     1561 cgacctgctg gcgccgccag cggttgcgca aggcgatggc cacggcccgc ttggcgtcgt
     1621 gctggccgac gatgtggcgg tcgagttcgg agacgatctc ctggggggtc atggaggaca
     1681 tggcgggagc gtgacagatg acaaacgact tcgtcgcggc gcgacgtcaa tggcgaacga
     1741 cacgaggtca ttcgccattc gtcatggttc agagaacctc gatggtgtgc tgcatgttgg
     1801 tgtagatgca caactcgcca gcgatttcga gcgacttccg gacgatctgc tcggccggca
     1861 ggtcggtgtg cgccagcagc gcccgggccg ccgcctgggc ataggcgccg cccgaaccga
     1921 tggccaccag gccgtgctcg ggctccagca cgtcgccgtt gccggtgatg atcagcgagg
     1981 cctcgcggtc agccaccgcc agcatcgcct ccagccggcg cagcacgcgg tcggtacgcc
     2041 agtccttggt gagttcgatg gcggcgcgcg tcagatggcc ctgatgtttt tcgagcttgg
     2101 cttcgaagcg ctcgaacagc gtaaaggcat cggcggtggc gccggcaaag ccggccagca
     2161 cccgaccgtg gtgcagcttg cgcaccttgc gcgccgtgcc cttgacgacg atgtggccca
     2221 gggtgacctg accgtcaccg ccaatggcca cctgccagcc ctgcggggtc tgacgccgca
     2281 cgctgacgat ggtggtgccg tgaaatgctt ccatatcttt tccagatgat ggtcagctgt
     2341 tgcgattcaa gtgcgggccc ttcccgcgcc tgtacccttc aatccgtgcg cagcagcaca
     2401 cgcgaaacgt cggtgatgcc aatgacgccg aagaacgccg cgaccagtcg gttcacctgc
     2461 ttgaagctga tgctgcgacc actcccctgc tgctcggtgg cccagttcag caggtcacct
     2521 gcggcgccga aatccacccg gaccaactgc ctgcagttga actcgaaatg ccggacctca
     2581 ccccgctgcg ccaagggctg cagcaaggca ctcatggagc cactgtaggc cccgtccagc
     2641 actgtcctga cgaccccctc aggtggctct tcagtgtcgt gcgacggcgc ctcatcccca
     2701 cctggctcgg gcattctcac ccctagggta tcgctgccga accgactcga catcagggtc
     2761 tcgccctcct cgttcatgcg ggtccagccg ttgaccggat cttcccacgc cggcggagac
     2821 acctcgtaag tgacacagta gttgagtgcc accagatcga actcgtccat ctgcccgagc
     2881 acgcgcagca gcgccaggcg ggcctcccac cactgcggat cgaccgtgcg atcgccggtg
     2941 ggtgaacgct cggtcaacac gtccatcaga cgctcgatgc ccagcagctt cagccgcacc
     3001 ggcgtccttc cccagcgctg cagctgctcg accaacgacg gcagtgcggc tgcctcgatg
     3061 gactggaggt gacgccagtc gagacgccag ggcggcgcgt ggcgctccag cgacatgcgc
     3121 agggtcgcca tcgattgcgt acccaacgca gacggactga tccagtggaa cggcccggcg
     3181 gccgtggccc ccggcgctgg cgcagccgtc aacgggctca cccccgagga cggccgatcg
     3241 agcatgcccg tccactgcgg cgccgaacgt ccgaactggg ccgcgaactc gggcgcggcg
     3301 tcgtcgaacc tcgcctgatc accgatacat cgatacaggt cgaacagcgt cagccaggta
     3361 tcgacatcct cgcgtcgggc accgccttca gcgaccaacg cgcgcagcag cgcctcggcg
     3421 ccggcgccat cgccattggc aaacaggatc gcggcctcct cgatctcagg atcctggcgg
     3481 ccggcggcca tcacttcaac gttgaagtcc tgcacctcgc ccagccaggg gatggcgcgc
     3541 gcagcggctg cccctcccgc tgatggatgc agtggctggg cggacaaaga ctccttggcc
     3601 aaggctgacg ccgacccgtc tgccgcggcg tccagcagcg gcgcgccacc ctccggcggc
     3661 ggtggcagtg gcatcgggtc ggtcttgtcg taggcgtgga ccgacggctc cgatatggca
     3721 tccgccgccg gcccggcagc ttgattcttc cccactcgat gcctggacca tgcggtcgac
     3781 atctgcgcct cgatctcgtc gatcttcttg agcgtgagct cccgttcgcc ggtattggcg
     3841 ggatggctgc tggggtaaaa tgaagggggg cccgcggcaa ggtcgctgcc cttcagcgtc
     3901 tcgcggcggc gcagcttgcg cagcatgtcg aactcgcgac tgcgcacaaa atcgttgcga
     3961 cgtttgcgct cgatcatctc cttgagcgcc agccgcgact ggctgtcgcc atcctcggaa
     4021 tcgggctgat cgagatccgc ccagtgcgtg gtggggttct tgacgaaacg taccaccttc
     4081 gaaagcagtc cacccggctc ggtaggcttg ttcatggctc ggtacctccc gtggtcagtg
     4141 catgccctgc ggcggcgcga gattcagaga cgaagcatgc ggtcgcagca gcgtcggcga
     4201 gcctgcggtg gccgcaccgc acggcaccac catggatcgc accacgaccg gcgaccgcct
     4261 gcgcggtgcg cggcgctgca gcacggccgt cgggcatgaa cccatcgtct cgcctcggat
     4321 cagtcgccga acattttctg tttcagctca cgccgctgct gggcctccag cgacagcgtg
     4381 gcggtcgggc gcgccagcag gcgcggcacg ccgatcggct cgccggtttc gtcgcagtag
     4441 ccgtagtcac cggcatcgat gcgggcgatc gattgctcga tcttcttgag cagcttgcgc
     4501 tcgcggtcgc gcgtgcgcaa ctccagcgcg tgctcttcct cgatggtggc gcggtcggcc
     4561 gggtcgggca ccaccaccgt gtcgtcgcgc aggttctcgg cggtctgatc cgcgttcgcc
     4621 agcatgtctt ccttgagctg gaccagcttc aggcgaaagt aggccagctg cttgtcgttc
     4681 atgtactcgt tgtccggcat cgcaatgatt tccggatcac tgagctgttc gaccgacttg
     4741 gtcttccagt tgttggccag cttggggtct ttcttgaccg gcgcgggcat cgggggggtg
     4801 gcaacggagg gcatgggcag attcctggat accggctgcg gcggcttggt cggcacggcg
     4861 gtcgacgcct tggcggcggc cgagcggacc ggtgacttct tgctcggctc aggggcggac
     4921 acccgggcct tgctggccgg cttgccggcg ctcgaaggtg ccttggcggg tttggccttg
     4981 ggggttgccg ggctcttggc agccgcagtt ttcacttgtc tcgtctcctg ccggcaccga
     5041 cctccagggt ggcgccgacg acatggccag cagatgaagc cgggtctgcc tcatccgatc
     5101 agcgccctgt tgtttgacct tcttgggcga tgcgggcaca gcgcccccgt tcaccggggt
     5161 tgcttacgaa ggcggcgatt gtagccacgc ccggccggcc tgggcacacc gggcaaacaa
     5221 atcgtcgacc ttgtgcgaaa accttgacgc aagccccaat cggatggtcc ggcggttcgg
     5281 caggcacgca ccgaatcccg catgccgaaa acgcgcaccg caacgcgacc ggtcaaggcg
     5341 cgagccccgg cggcaacacc ggccacggcg agcatctgcc gtgcggcgac ggagcgggtg
     5401 ggcacaccgg gcgggcatga aagacccaac cacccctgtt caggcggaga ccagacactg
     5461 ctccaggccc tgggtgaaga tgtcgcgcgg caggtcgatc ccgatgaaca ccatcttgct
     5521 ctggcgcggc tcgtcgccct tccaggccgg ccccagatcg ctgcccatga gctggtgcac
     5581 gccctgaaag atcaccttgc gctcggtgcc cttcatccac agcacgccct tgtagcgcag
     5641 catgcgcggg ccgtagacgt tgacgatggc gcccagaaag tcctccagcc gcgccgggtc
     5701 gaaggcacgc tcggcgcgga acacgaagct cttgacgtcg tcgtccgcgt ggtggtggtg
     5761 gccgtgcgcg tgctggtgcg acgggtggtc gcagtggcca tcgtggtggt gatggtggtc
     5821 gtgatgatcg tggtcatggt cgtcggccgc cagaaattcg gggtcgatgt ccagtcgggc
     5881 gttcaggttg aaaccgcgca gatcgaacac ctcggcgatc ggcaccgcgc cgaaatgcac
     5941 caccttctgc gccgcgcgcg ggttcatgtg cttcaggcgg tgctgcaggg ccgccagctc
     6001 gcctgcatcc accagatcgg ccttgctgat gaagatctgg tccgcaaagc cgacctggcg
     6061 ctgcgcctcg atgcggtcgt tcagctgttg cgccgcgtgc ttggcatcga ccagggtgac
     6121 gatggcgtcg agcaggtagc cttccgcgat ctcgtcgtcc atgaagaagg tctgggcgac
     6181 cggaccgggg tcggccaggc cggtggtttc gatcaccacg cgctcgaagt cgagctcccc
     6241 gctgcgcttt ctgcgcgcca gatcggacag ggtggcgcgc agatcctcgc ggatgctgca
     6301 gcagatgcag ccgttgctca tctggatgat ctgctcctgg ctgtcgctga ccaggatgtc
     6361 gttgtcgatg ttctcctcgc cgaactcgtt ctcgatcacg gcgatcttct ggccgtgcgc
     6421 ctcggtcagc acgcgcttga gcaaggtggt cttgccggag ccgagaaagc cggtaaggat
     6481 ggtggcggga atcagtgaca tggcagggtg cgggcgtggc gcccgccgat gggagaagac
     6541 gtcgaacacg gcagtgtagc cacccccatc caccgcggct gcggctgcgg cccgcatgcc
     6601 caagtgttgc ccgaatccac gatcagaagc tccgccaccg gaaaagcgca agtccaacga
     6661 gctgtcaatc gtaaacctta gttgtatttt tttgtagatt gcggttcaat ggtgccgaag
     6721 cttcggtctg caacgcggtc aacgcgcacc gtggccaccg cttgctggga ccccaagcga
     6781 cttctccact caccgtgcat ccatgtccaa gtctttcaac cttgtggccc tcgccctcag
     6841 tctgggcatc accggcgctc aggcgcgcga gctcgtgctg ggcatcagtg aaggcacctc
     6901 gggcggcacc gaccacgccc gagtcgtgca gaagtacggc ggtctggccg aagccatagg
     6961 taaggccatc aaggccgagg tgaaggtggt gttcgtgcgc gagtttgcac agttggagga
     7021 tgggatgaag agcgcccgcc tcgacctggt gatggcgcgc ccgagcgact accctgcgcg
     7081 cggcttgcgc gactacggct atcactatgt ggccacggtg aaacccgaag gacagtgcct
     7141 gatcgtcgtg cccaaggatt cgcccatcaa acagctggca gacctcaaag gcaagaagat
     7201 cgtcatgcca gagaaggtcg cctatatgac caaattctgc aaagccgagt tgcgccacca
     7261 gggcgtcgac ctcgacaagg agagcgtcac ctacgtgcgc gaacaggagg ccgtggtgtt
     7321 ctacatcaac aacggtttcg ggcaggtggg tgggctggca tcctattcgg gggcggcgcg
     7381 caaatggctg aaggaaggtg gcaccgtgct gcaccgcagc gtgccgcagc cctattcgcc
     7441 actgattgcc tccaaggcgt tgagtgcggc cgacattgcc gccgtgcaga aagcggtgct
     7501 ggccttgcct gacagcccgg aaggccaggt ggtgctgaaa tccatcggca tgccgggcgg
     7561 ctttgacacc gccaccgaag acaagttgcg cggactgctc aagtttctgg gcgactgatc
     7621 acggcctgcg cggccccgtt gctcagcgcc gtcgcgcgcg cgggtgggcg gcgtcgtagg
     7681 cctgcgccag gtgctgaaaa tccagtcggg tatagacctg cgtggtgccg atgctggcat
     7741 ggcccagcag ttcctgcacg gcgcgcagat cgccgctgga ctgcagcaca tggctggcga
     7801 aggagtggcg cagcatgtgc ggatgcaccg gcgtggccag ccccgcctgc tggctgcgcc
     7861 ggcgcaggcg ctgccagacc gatggcgcgc tcagacgggt gccgcgccgg ccgatgaaca
     7921 gcgccgccgc ggcctcgccc gcggccatca cctggccgcg caccgccagc cagcgccgca
     7981 gggcttcggc ggccgagcgt cccaggggca cggcgcgccg cttgctgccc ttgccctgca
     8041 cctgcacctc ggcctggtcc agatcgatcc agccacggcc agcgcgtgcg gcggcgtcgc
     8101 tggccgccac gtccagcccc accagctcgc ccacgcgcag accgctgccg tacagcagct
     8161 ccaccatggc ggcgtcgcgc gcgtccagcc aggggtcatc gccctcgtcg atgtgttcgg
     8221 ccagttgcac cgcttcgtcc acccccagcg ccttgggcag cggccgcggc tggcgtggcg
     8281 cccggatgcc cagcaccggg ttggcaccga tccctccttc gcgccccagc cagacgtaaa
     8341 agccccgcca gcccgacagg atcaacgcaa tgccgcgccc gctgcgaccg ccggcatgca
     8401 tgcgcgccac ccaggagcgc acatggtggt gctgcacctg gggcagcgcc acaccggcct
     8461 gcgcggccag gcgggcaagc ttgtccagat ccgctcgata cagcgcgagc gtgcgtgggg
     8521 ccaggcgctt gagcacccgc gcatgggtga ggtagcgttc gaccagcgcg gcgtcggccg
     8581 cggcggcgga cgggaggggt tcgacaaccg atgacatgct atttgaatga tagctttttg
     8641 cgcagtcgga tcaagcacca gagcccacat tcaacctgga aacggcagtt gaccgcttgt
     8701 ccgctgcggg cagaccgcat cgatgcgggg tctggcggct gcgatggcct tcacctcttg
     8761 cgtgagcacg ctacgcaccc acccgcagcg catcgacgcc cggttgccca acccgaccgg
     8821 gcgccgcccc ggcgcggcgg cgctgctagc atcgcaagca tgaccgccca cgacgaccgc
     8881 gtcaccgacg tgcagcccaa gcgcgaaacg ccgcgccccc cggcgcacgc cccggtgccg
     8941 cgtgccgagg ccaccgccgc cgcacgcccc gacgcccgat tcatggccgc gcacccggcg
     9001 cacgcgctgg cgctggggtt cggcagcggc ctgtcgcccg tggctcccgg cacggccggc
     9061 acgctgtggg cctggctggc gtggtgggca ctgggcctgt ggctgagccc ggcgcagcag
     9121 ggctggctgc tggtcggcgc gctgccgctg gcctggtggg cctgcaccgt caccgcgcaa
     9181 cacctgcaga cacccgaccc gggtgccatc gtcatcgatg aggtggtggc gttctggctg
     9241 gtgctgtggc tgctgctgcc ggcggggttc tggggccagg ccacggcctt tgcgctgttt
     9301 cgcttcttcg acgccgtcaa gccgggaccg gtgggctggg ccgaccgcct gctgcacgga
     9361 cgccgcggct gggcgggcgg cttcggcgtc ctgctggacg acctggtggc cgccttctgc
     9421 accttgctgg tgctggcact gtggcgcccg gggtgagcac cgatgctacc aaaacaagag
     9481 ctttcggcgc ttgctggatc tgcgccagag gccaaaatcg ttcatgacat cgcagccctg
     9541 ctgaccgccc gcggctggtt gctgacgacc gccgagagct gcaccggcgg cctggtggct
     9601 gccgcctgca ccgagctggc cggctcctcg ctgtggttcg aacgcggcat cgtcagctac
     9661 agcaacgccg ccaagagcga gttgctgggc gtgccgccgg cactgatcgc gcgccatggc
     9721 gcggtcagcg agccggtggt gcgcgccatg gccgaaggcg ccctggcgca cgcgcgcgcg
     9781 caggtcagcg tcgccctcac cggcatcgcc gggccgaccg gtggcagcgc cgacaagccg
     9841 gtcggcaccg tgtggttcgg ctggtgtgtg gacggacgca ccgacaccca gcggtgccgg
     9901 tttgacggcg accggcaggc cgtgcgcgcg caggccgccc tgcacgccct gcgcgggctg
     9961 gccgcccgcc tggcggcgcc tggtggcgcc gcgaccccgc tcacttgacg gcgatcaggc
    10021 gccagtcgag ccggttgtcg gcgcggtgca cgacctcgat gttcttcttc atcgcccagg
    10081 gaatgacctg gttgtgcagc ggcaggtgcg acacgtcctt gtgctccagg atcagggcct
    10141 gctcgatcag ctcggcgcgg cgcttctgat cgacctcgac cttgacgcgc tcgaccagcg
    10201 cgtccatctg cgggttggag tagcgcccca cgttgtagtt gccgtcgccg cccgcaccca
    10261 cgctgcgcac cagcgactgc agactgtaga gcgcatcgaa ggtcggcacg ccccagccca
    10321 gcatgtagat gctggcctcg tggcgctgga tcatcgggaa gtagctcacc agcggcatgg
    10381 tgcggagctt ggccttgaca cccaccttgg accacatcgc ggccaccgcc tggcagatgc
    10441 gctcgtcggc gatgtagcgg ttgttggggc aggcgaaatc gacctcgaag ccgtccttgt
    10501 aaccggcctc ggccagcagc ttctgggccg ccgccgcgtc gtacggatag cgctggtcgg
    10561 cgcgcttggt gtagccgctc acctgcggcg cgatcagcgt gccggtgttc tggctcaggc
    10621 cgcgcatcac caccttgtgg atggcgttga tgtcgatggc ctggtacagc gcctggcgca
    10681 cgcgctggtc cttcaggggg ttcttgcctt tgacgctgga gcccggcagt tcgtcgcggt
    10741 gctgatccat gccgaagaag atggtgcggt tctccggccc ctgtaccacc ttgagggccg
    10801 cgttgcgctg cacgcgctcc agatcctgcg gcgtcgggtc gagcacgaag tccacctcac
    10861 ccgacagcag cgccgccatg cgcgtggcct cggacttgat cggggtgtag atgacctcgg
    10921 tgacgttgcc cggcttggcc ttggcgccat gcccccacca ctgggggttg tccaccagca
    10981 ccagcttctg gtcgggctgc cattccttga ccatgaaggg gcccgtgccc atggcgttgc
    11041 ggtgggcaaa gctctcgtcc ttggtcttga tgtccttggg ctcgaccgac ttgttcttct
    11101 cggcccaggc cttgctcatg acgcgcagtt cggtgagctg attgagcagc accgggttgg
    11161 gcgccttggt gaagatgtcc agcgtgttcg gccccgccac caccaccttg tcgatgccct
    11221 gcgtgtagac gctgtagttg gaggtcttgg ccatggcgcg ctcgatggag aacttgacgt
    11281 cctcggcggt catgctgctg ccgtcgctga acttcacgcc ggtgcgcagc gtgaactgca
    11341 tctgggtcgg actgacctgc ttccaggccg tggccagcat gggctcgatc ttgaagctct
    11401 tgctgttgta gtagaccagc gactcataga cggcggcgtg cacgccgttg cccagcgcgt
    11461 tgttctgcga atggatgtcc agcgtcggaa tgtcgctggc gctggtccat ttgaaggtcc
    11521 tggcgtgcgc acccagggtg cagcagacgg ccagtgcggc cacggcgagc gtgcgaagct
    11581 tcatatcgga tgtcctccag ggtgatgggc aaagtcgcgc agtatgccgc gccgccgcca
    11641 gaccacccac agggaagtcc ctgaagcgcg ccggcggccc ggctcgcacc gccgcatgaa
    11701 cccggcgcgc ggcgaggaaa accctaatcg gtcggccgcc gccccacccc cataatgccg
    11761 cgttcccgga acttgaacaa agcagtcgaa gacatgagtt cggacatcat tctggaaacc
    11821 cgtgggctgg tgaaggagtt caagggtttc gtcgccgtca acgacgtcaa ccttcaggtg
    11881 cgcaagggca ccatccacgc actgatcggc cccaacggtg ccggcaagac cacctgcttc
    11941 aacctgctga ccaagttcct ggagcccacg cgcggccaga tcctgttcga cgggcaggac
    12001 atcaccgccg agcggccggc ccagatcgcc cgccgcggcg tggtgcgctc gttccagatt
    12061 tcgtccacct tcccgcacat gagcgtgctg gaaaacgtgc gcgtgtccct gcaacagaag
    12121 ctgggcacct cgtttcactt ctggaagtcc gaacgtaccc tggaccagct caacgagcgc
    12181 tgcatggagc tgctgggctt cgtcggcctg gccgacttcg cccacctgga ggcggtggag
    12241 ctgccctatg gccgcaagcg cgcgctggag atcgccacca ccctggcgat ggatccgaag
    12301 ctgatgttgc tggacgagcc cacccagggc atgggccacg aggacgtcga catggtcacc
    12361 cagctgatca agaaggtggc cgccaaccgc accgtgctga tggtggagca caacatgagc
    12421 gtggtgggca gcatcgccga ccagatcacc gtgctgcagt tcggtcaggt catcgcggaa
    12481 gggccgtacg ccgaggtgtc gcgcaacccg caggtgctgg aagcctacat gggcagcgcc
    12541 gaagaagccc tgcagggcgc gcatggttga ggacagtcac cgcatgagcc acacatccgc
    12601 tcccgccatc gtcctcaagg acgtccattc ctggtacggc gaatcgcaca tcatgcacgg
    12661 cgtccacctg gaggtgaagc cgggcgaggt ggtcaccctg ctgggccgca acggtgccgg
    12721 tcgcaccacc accctgcgtg ccatcatggg cctggtgggc aagcgcaccg ggtcgatcca
    12781 ggtcaacggg gtcgaaaccg tcaagctggc gccgcaccac atcgcccgcc tgggcctggg
    12841 ctattgcccg gaagagcgcg gcatcatgac ctcgctgacc tgcgaggaaa acctgatgct
    12901 gccacccaag gtggccgaag gcggcatgag cgtggacgag atctacgaca tgttccccaa
    12961 cctgaaggcc aggcgcaaca gcccgggcgg ccggctgtcg ggcggcgaac aacagatgct
    13021 ggcggtggcg cgcattctgc gcaccggcgc caaggtgctg ctgctggacg agatctccga
    13081 aggcctggcg ccggtcatcg tgcaggcgct ggcgcgcatg atccgcgagc tgcgcgcgcg
    13141 cggcttcacg gtgctgatgg tggagcagaa cttccgcttc gccgcaccgc tggccgatcg
    13201 cttctacatc atggagcgcg ggctggtgct gcgcgagttc agcgccgccg aactgcagga
    13261 caacatggac atgctgcacc agtacctggg cgtgtgagcc gtgaccaccg cccctatgga
    13321 accccgggcc tgccgccgat cgatctgact tctttgcccc cagaggaaca caccccatga
    13381 aacgcaagac actgtgcgcc ctgatcaccg gcctgggcgc cgcgttcggc ggcgccgcgt
    13441 acgcccagat ctcgggcggc gtcgtcagga tcggcgtgat gaacgacatg gccggcccct
    13501 atgccgacct ggccggcccc ggttcggtgg tcgccgccag gatggcggtg gacgactacc
    13561 tcaaggccac caactccaaa ctcaaggtgg aaatcatctc cgccgaccat cagaacaagc
    13621 ccgacgtcgg ctccagcatt gcccgcaagt ggtacgactc ggacggcgtc gacatgatcc
    13681 tggacgtgcc cacatcgtcg gtggcgctgg cggtcaacga agtcaccaag gaaaagaaca
    13741 aggtgatggt caactccacc ggcggcaccg ccgacctgac cggcaaggcc tgcacgccca
    13801 acaccgtgca ttggacctac gacacctgga tgctggccca cggcaccggc tcggcggtgg
    13861 tcaagcaggg cggcgacagc tggtttttcc tgaccgccga ctacgccttc ggccacgccc
    13921 tggagcgcga cacttccgac gtggtcaagg cctcgggcgg caaggtgctg ggcagcgtca
    13981 aggtgccgct gggcacggcc gacttctcct ccttcctgct gcaggcgcag tcgtccaagg
    14041 ccaagatcat cggcctggcc aacgccggca gcgacaccat caactcgatc aagcaggcgg
    14101 ccgagttcgg catcgtcaag ggcggtcaga agatggcggg tctgctggtg ttcctgagcg
    14161 acatccacgg cctgggtctg aacaccgcgc agggcctgca ggtcaccacc accttctact
    14221 gggacctgaa cgacgccacc cgcgcctggt ccaagcgctt tggcgccgcc caccagaacc
    14281 gcatgccgac catggtgcag gcaggcgtct acgccggtct gcagcactac ctgaaggcgg
    14341 tcgaggcgct gcagtccgac agcgacggcg ccaaggtggt ggccaagatg aaggagctgc
    14401 ccaccgacga tccgctgttc ggcaagggca ccatccgcgt cgacggccgc aagattcatc
    14461 cggcctacct ggtcgaggtc aaaaagccca gcgaatccaa gtacgcctac gactattaca
    14521 aggtgctgca gacgatcgcg gcggacaagg ccttccgacc gctgaacgag ggcaactgcc
    14581 cactggtcaa gtaaacccat cccctgtcac cccttttttc acccggagga ctttcgatga
    14641 agcgcaacgc actgtggtca ctgttggctg gcctgggtct gctggcggcc ggctcggccc
    14701 acgcccagat ttcgggcaac gtcgtcaaga tcggcgtcat gaacgacatg tcgggtctgt
    14761 acgccgacat cggtggacct ggctcggtga tcgctgccaa gatggcggtc gaggactacc
    14821 tgaaggccac caagtcctcg ctcaaggtcg aggtggtgtc ggccgaccac cagaacaagc
    14881 ccgacgtcgg ctccagcatc gcccgcaagt ggtatgagac cgacggcgtc gacatgatcg
    14941 ccgacgtgcc cacgtcttcc gtggcgctgg ccgtcaacca ggtcaccaag gacatgggca
    15001 aggccttcgt caacaccggc gccgccacct ccgacctgac cggcaaggac tgctcgccca
    15061 acaccgtgca ctggctgtac gacacctgga tgctggccaa cggcaccggc agcgcggtgg
    15121 tgaaggccgg cggcgacagc tggttcttcc tgaccgccga ctacgccttc ggccacgccc
    15181 tggagcgcga cacctccgaa gtggtcaagg cctcgggcgg caaggtgctg ggcagtgtca
    15241 aggtaccgct gggcacggcc gacttctcct ccttcctgct gcaggcacag tcgtccaagg
    15301 ccaagatcgt cggcctggcc aacgccggcg gcgacaccat caactcgatc aagcaggcgg
    15361 ccgagttcgg catcgtcaag ggcggccaga aactcgccgg tctgctggtg ttcctgcccg
    15421 acgtgcacgg cctgggtctg aacaccgccc agggcctgaa catcaccgag gccttctact
    15481 gggacctgaa cgacggcacg cgcgcctgga gcaagcgctt tgctgccgcg cacggcggca
    15541 agcacccgtc gtccgaccac gccggcgtct atgccggcgt gctgcactac ctgaaggcgg
    15601 tggacgccgc caagaccgac gacggcagca aggtggtggc caagatgaag gagctgccca
    15661 ccgacgatcc gctgttcggc aagggcacgg tgcgcgccga tggccgcaag atccacccgg
    15721 tgtacctgtt cgaggtcaaa aaacccagcg agtccaaggc cccgtacgac tactacaagc
    15781 tggtgcagac gatcgcggcc gacaaggcct tccgaccgct gaacgagggc aactgcccgc
    15841 tggtggccaa gaagtaagtg cgagcagccg caaggcatag acaaccatgg aaatcttcgg
    15901 tatcccgatc caggccatga tgggccagtt gctcatcggc ctcatcaatg gctcgttcta
    15961 tgccttgctc tcgctgggcc tggccgtgat cttcggcctg ctcaacatca tcaactttgc
    16021 ccacggcgcg cagtacatgc tgggcgcctt cggcgcctac ctgctgctca acaagctggg
    16081 gctgggctac tggtggtcgt tgctggtggt ccccatcctg gtgggcgcca ccggtgtcgt
    16141 catcgagcgc accatgctga aaccgctgta caagctggac cacctgtacg gcctgctgct
    16201 gaccttcggc ctggccctga tcatccaggg cctgttccgc aacgagtacg gctccaccgg
    16261 cctgccctac gccattcccg aggcgctgca ggggggcaag aatctgggct tcatgttcct
    16321 gcccaattac cgcgcctggg tgatcgtggc ctcgctggtg acctgcctga gcacctggtt
    16381 cgtgatcgaa aaaacccgcc tgggtggcta tctccgtgcc gccaccgaaa acccccagct
    16441 ggtgcaggcc ttcggtatca acgtgccgcg catgatcacc ctgacctacg gcttcggcgt
    16501 ggccctggcc gcgctggccg gcgtgatggc agcgcccatc taccaggtca gcccgcaaat
    16561 gggcgaaaac ctgatcatcg tcgtgttcgc ggtggtggtg atcggcggca tgggctccat
    16621 catgggcgcc atcgtcaccg gcttcgggct gggcctgatc gagggcctga ccaaggtgtt
    16681 ctatcccgaa gcctccacca ccgtcatctt cgtcatcatg accatcgtgc tgctgatccg
    16741 cccggcgggt ctgtttggcg cgcagaaata aagccatggc cagcgcccac aaccccaccg
    16801 cgcctgccgc cgcgccggca gcccaccccc ggaacgccag cgatccggca cgcacccaca
    16861 gggtggcctt catcctcatg gccctcatct tcatcggtgc gccgctggtc ggcgtgtacc
    16921 cggtgttcat gatgaaggtg atgtgttttg ccctgtttgc ctgcgccttc aacctgctgc
    16981 tgggttacgg cggcctgctg tcgttcgggc acgccatgtt tctgggcagc gccggctacg
    17041 tgtcggcgca tgcggccaag gtctggggcc tgacgccgga gctggccatc gccctgggtg
    17101 cgtcgtgcgc cggcgcgctg ggctgggtcg ccggcaagct ggcgatccgc cgtcagggca
    17161 tctacttcgc catgatcacc ctggctctgg cgcagctggt gttcttcttc agtctgcagg
    17221 cgcccttcac cggtggcgag gacggcatcc aggcggtgcc gcgcggcaag ctgttgggcc
    17281 tgatcgacct ggccaacccc gccgccatgt accccttcgt gctggcgatc ttcctgggcg
    17341 gctttttgct gatctaccgc atcgtgcatt cgcccttcgg tcaggtgctg aaggccatcc
    17401 gcgagaacga ggcgcgcgcc atttcgctgg gctacgacgc cgacaggttc aagcaccgcg
    17461 ccttcgtgct gtcggccacg ctggccgggc tggcaggtgc caccaaagcc atcgtgttcc
    17521 agctggcctc actcaccgac gtgcactgga gcatgtcggg cgaagtggtg ctgatgaccc
    17581 tggtgggcgg catgggcacc ttgttcggcc cggtggtcgg ggcggccgtc atcgtctcga
    17641 tgcagaacta cctggcccag ctgggggcct gggtgacggt ggttcagggc gccatcttcg
    17701 tgctgtgcgt actgctgttc cgccgcggca tcatcggaga aatcgggcac ctgctgaaga
    17761 aatcgctctg agcctgcccc ttgcgtcacc ccaagcgccc gtcacgggcg cttttttgtt
    17821 cgatggaggg cgctgaaacc cggctcgttc ccgacccgat ccgggtggcg gctcactcga
    17881 ccaccgtgcg cgactgttcg cgcaggtact gcggcaggta gtagaacgag ccgatggctt
    17941 tgccggtgct gccgctgccc ttccagccgc caaacggctg gtagcccggc caggcaccgg
    18001 tggtggcgcc ctgcgggcgg ttggcgtagg tgacgccagc ttcgatgtgc cgatggaaat
    18061 aggccacctc gtcgggcgcg ccatagaagc ccgcggtcag gccatagtcg ctggcgttgg
    18121 cgcgggcgat gccttcggcg accgatgcca cttcgcccac cagcaccagc ggcagaaaat
    18181 gctcgtccac ccaggcgcga tcgtccagtg gcgcctgcgc caccgtggga gcgacgaaaa
    18241 atccacgcgc cagcgcgccc tcggtcagcc agcgcccgcc ggtgtcgatg cggccgacct
    18301 gccgcagatg ctcggccagg cccgcgtagc gctcgtaagc ggcgcggccg atgaccggcc
    18361 ccatccagtg cgcctgctgc gtcggatctc cgaccgacag ctcgcccgcc agccgcacca
    18421 ggcgggcacg cagatcgtcc gccacgggct gctccacata gacgcgcgag caggccgaac
    18481 atttctgtcc cgacagaccg aacgccgaac gcacgatgcc cagcgccgcc cgctccaggt
    18541 cggcgcggcg gctcacgatg gtggcgttct tgccgcccat ctcgacgatg cagggtcggg
    18601 ggaagggccc ctgcaccgac tggcgcaaca cctgcatgcc gaccgcctgc gagccggtga
    18661 aggtgatgcc cgccacgccc gggtgctcga ccagcgcgcg tccggtggcg tctcctccgg
    18721 tgacgaagtt gataaccccg gccggcagac cggcgtcgtg cagggcgtcc agcagcagcc
    18781 gcccggacag cgaggtttcc ggcgacacct tgaacaccgc cgtgttgccc gccaccagcg
    18841 ccggtccgag cggcccgccc gccagtgcaa acggaaaatt gaacggcgcg atcaccgccc
    18901 acacgccgta ggggcgcagc acggtgcggt tgtggctgcg aaactgcggc agcgggtcgt
    18961 ctggcagcgc gcggtcaaag ccgtcgtggg cttccatctg atcgcaatac cagtcgatca
    19021 ggtcggccgt ctcctgcacc tcgccgatcg actccatgcg gttcttgccc acctccagcg
    19081 ccacggcggc gctgatggca tagacccgct cttcgatcag gcgggcggca cggcgcagca
    19141 gcgccacgcg ctcgcgccac ggcgtggccg accagcccgg ataggcggcc tgcgcggcct
    19201 gcaccgcgcg gtcgacgtcc tgggcgctgc cctccacgaa gcgcccgatc agccagtcct
    19261 ggtcaatcgg tgaacgcacc tcgaaatgcc gcgccccacc caccgacatg ccaccgatcc
    19321 actgtgcgtg gtcgcgcccc atgccgtcgc gccgaaagct ggccagcgcc gcgtcgaagc
    19381 gcgcatgcag ctccggcggc ggattgaaca tggtcgaata ggtcagcttg aagccggaac
    19441 cagcgggcat gtgaacctcc agaagactgc attcaaccaa aaaacgaccg tggggcgcgc
    19501 ccacccggca gacgagggtc agccaagccg agccaaccgg tgttcgcgtt caccgcggcg
    19561 gatccgccgg gggacgcagc gccggcacat gctggcccca gcccagcagc aaccacaacg
    19621 ccaccagcgc cgacgtgccc gacagcaccg ccgccggtcc ccagcccttg agcagccagc
    19681 cgcccagcgc cccgccggca aacaggccca gcgattgcag ggtgttgtac acgcccaggg
    19741 cggcgccgcg cgcctgggcc ggcgccaggc gcgagatcag gctgggctgg ctggcttcga
    19801 tcaggttgaa ggccatgaag aaaaagaaca tcaccacgcc cagcgtccac agccccggcg
    19861 cgcgcgaggc cagcagcagc agagccagct gcaccagcag cagcagggca atgccgccgc
    19921 gcagcacggc gcgcaggtgg ccgcgccgct ccagcgcgaa cagaccgccc atgaacacga
    19981 acgagaccag cacggccggc aggtacagct tccagtgctc ggccttcagc agcccggcct
    20041 gcaccagcaa ccctggcacc accacccaca tcgccatctg caccgcgtgc agcacgaaca
    20101 cgcccaggtc cagccgccac aggtggggat ggcgccacac ctcctgcaac cgcccgcgcg
    20161 gcgcatcgac gtgctgcacc ggttctgccg gcgtggccac cagcaccagc accacaccgg
    20221 ccagcgccag cgccgccgtc agccagaaca gcccggacag gccgatcgcg ccggtgagcg
    20281 gcggcgccgc catcagcgac agcgcaaaca tggcggcgat gctgatgccg accagcgcca
    20341 tcgccttggt gcgcacctcg tcgcgcgtca ggtcggccag cagcgccgtc actgccgccg
    20401 acacggcacc cgcaccctgc agcgcgcgcc cggccaccag catgtgcacg ctgccggcca
    20461 gcgccgccag cgcgctgccc agcgcaaaca ccagcaaccc ggccacgatc accttcttgc
    20521 gaccgatgcg atcggacgcc agcccgagcg gcacctgcat caccgcctgc gtcaggccat
    20581 agatgcccat ggccagaccc accagtgccg ggtcgtcgcc gcccgggtag cgcgccgcct
    20641 ccagcgcgaa caccggcagc accagaaaca gtcccagcat gcgcagggca aagataccgg
    20701 ccagcgacag gctggcgcgc cgctcgtcgg cggtcatgcg gtggacgtgg gatgcgtggg
    20761 cagcaggcgt ggcggaggcg gacatcgaca aacggcgcag aaaacgacgc cggctgtgcg
    20821 ccggccagcg gtattgtcgg tgatcgtgga tctgctagat tgccggatca tgaaagccac
    20881 ccaccacctg ccccgcgcca ccgcagtgct ggcgctggcc tgcaccctgc ttgccgcctg
    20941 tgcccagcag cccgccgccc ccaacccctc ggccgcccag gaactgaagg cgtttcccgc
    21001 caccctgaaa ggccaccagc gccacgtgat cgagctgccg gcgctgcccg atgaggccga
    21061 tcgccagatc gagctgatcg gcaccaaggc catgacggtg gactgcaaca cccgtggcat
    21121 ggacggccgc ttcgagtcgc gtgacgtgca gggctggggc tacaactact gggtgttgca
    21181 aagccgcggc caggtgatgg gcaccatgat ggcctgcccg cccggcagcc agcgccccgg
    21241 cgtcgcgcag acccagggca cgctgctgcg ctacaacagc aagctgccgg tggtggtgtt
    21301 cgtgcccgag ggcatggggc tgcgctggcg cgtgtggagc gccggcgcca tgcaagacgc
    21361 gtcggctcgc tgagagcggc cccaactgtc atctttcata gctgctcgcg cttgccaggc
    21421 aagcgctgca gcctgattcc tcttgcagca acacgcccga ccaggcgtgc ccgcgcgaag
    21481 ctcgtcgtcg cttgccagga cgacacgcgc gcactgcggt cgatcaccgc ttgtgggccg
    21541 gcagcgatcc gcgcagcatg cgtgtcacct ctccgcgtcg acacccggtc gatgcccgtg
    21601 atggatacac ggcacgcaca cgccgtatcc tcggagggtg gcatctggcc ggaggatcgg
    21661 catgtccttt cttcaaagca cttccaacac cgccttcttc attgcactgg gcatcgttgt
    21721 gctgctctcg ggcatcggct ttggcatcgt ggcctgggtc ggccgcctta ccggtgcgct
    21781 gcgcggccgt gagcctgtgc ccatcaacat gctgacggaa ggctacctgt tggcgcgagg
    21841 caaggccagt ggacggacgt tgcgggcgcc gctgtcgggc cgtccgtgtg tgtggtggga
    21901 gatgaaggtg tgggagcgcc ggatcgaaat cgaccgtcaa gccagcagcg acgaacccga
    21961 tcggcgcgcc tcctggacct tgctgcgaca cgacgtgtcg cggcgcccca tccattgcac
    22021 tcaaggcttc gtgacttgca ccgtgcagcc cgatggcatg accctgaccg tcccctccga
    22081 ggtgcgcgac tggaagggca agcaatatcc gccggagaac cgcgacccgc cagtgcagcc
    22141 cggttcggcg ttctcctatc ggcaggtgca cgatacgggc ttcaccatcg tcggtggcaa
    22201 acttttgggc gaccgctatc gctacgtcga actgctcatc gcgtcgaacg ccgagctgtt
    22261 tgttctcggt cgtgttgaac gcgtggcagc ccgggcaggg gcggccagca cgcatgacga
    22321 tgcgccggca cccgagggcg aatccgttgc cccgttcgag caggcgcaac gggaggagaa
    22381 cgaggacgaa cagatgagcc cgagcatgcg cgaggtccag tggcgcatcg cgccggaaaa
    22441 gggtcgtccc tacatcgtgg ccacgcagcc gccggagcag tggatggcca cgatcaagct
    22501 ggccagtcag ggcggcctga tcgtgggcac cgtgacggca gcgttggcag ccttcatgct
    22561 ctgggcgcgc tatcagggcg gctgaaccca gcaggccgat ggcgtcggtg ctctggccac
    22621 ccgccgggcc gaggcatccc cggcaggctc agtttctggc gaatgtcttg gccagcggtg
    22681 cgtcgaacgt cacgctgccg gtggcgccca catcgccttt gagcgtccag ctgccggcca
    22741 ggcgctggtg gctgtcctgc ttgagctgca tggtggccgg ctgggcggtg tcggagtgct
    22801 gcaccatctg gcccttgccg acgaaccagt agcctaggcg cgggccgccg tcgaaatcca
    22861 ccgtgacgcc gctgtgcaga tcggaactca cgcagcccag ccgatcgcac ttctggatcg
    22921 cggacgtcag gtcgacctct gacagcacca gcctgcgcat gggcttgcca tcgaatgagg
    22981 ggccggccag caaataggca tagcgcggcg cgaccttgac cacgccggag cgcgcctgca
    23041 gggccaactc gccagacgcc tgctgcgcct gcgcgcctgc tgccaccagc gtgaccagag
    23101 ccaggccgat cgcggcccga cgggtggccg atcgcaagct gccgcaatgc atggatctca
    23161 tggcgatctc ctcggggtca gggcagccgc tcgcgcctgg cgcgcatggg caaggcgccg
    23221 cagcggcagg caggaatgct acatcggatc ggaaccctgc ggtgggcgaa tctgggcttc
    23281 atggtgtgcg ggccgggtgt cttgcgccac ggatcggccg catgcaagaa gcaacccaga
    23341 cagcggccag gtagggcgtg agcaggcacc aacgccccga tttcggataa tggccggttg
    23401 ccctgcgccc ggggccgtcc gccatgaacg atctcgccgc ccctgatttg tccgacgccg
    23461 aaggcctgcc gctggccacc gcactgcgtc agccgcgcat cagcgtgcgc ggagcgcgca
    23521 cgcacaacct caagaacatc gacatcgaca ttcccaagga ccggctggtg gtgatcaccg
    23581 gcctgtcggg ctcgggcaag tcgtcgctgg ccttcgacac gctgtacgcc gagggccagc
    23641 gccgctatgt cgaaagcctg tcggcctatg cgcggcagtt tctgcagctg atggacaagc
    23701 ccgacgtcga cctgatcgag ggcctgtcgc ccgccatcgc catcgagcag aaggccacca
    23761 gccacaaccc gcgctcgacc gtgggcacgg tgaccgagat ccatgactac ctgcgcctgc
    23821 tgtttgcgcg cgccggcacg ccccactgcc ccgaacacga cctgccgctc acggtgcaga
    23881 ccgtcagcca gatggtcgac gccacgctgg ccctgccgca agacacgcgc gtcatggtgc
    23941 tggcgccgct ggcgcgcgca cgcaagggcg aattcagcga gctgctggcg caaatgcagg
    24001 cgctgggtta cgtgcgcttt cgcatcgacg gccaggtggt cgaggccgat cgcctgccgg
    24061 tgctgcagaa gaacgacaaa cacgacatcg acgtggtgat cgaccggctg aaggtgcgcg
    24121 gcacggccga cggcccgggc ggtgcggcaa ccgccctggc cgcgcgcctg gccgacagct
    24181 tcgaagccgc cctgcgcctg gccgacggcc gcgcgatcgc gctggagatg gacgagggaa
    24241 aggaacacct gttcaacagc aagttcgcct gcccggtgtg tggccactcc ctgcccgagc
    24301 tggagccgcg gctgttttcg ttcaacgccc cggccggcgc ctgcccgacc tgcgacggcc
    24361 tgggcaccac cgacgtgttc gaccccgaac gcgtggtggc cttcccctcg ctcagcctgg
    24421 ccagcggcgc catcaagggc tgggaccggc gcaacggcta ctacttcgcg ctcatcgaaa
    24481 gcctggccaa gcactacagg ttcagcgtcg agacgccgtt cgaggagctg cccgaggcgg
    24541 tgcgccacgt catcctgcac ggctcgggcg atgaggcgct ccggttcagc tacaccttcg
    24601 acagcggcgc gcaggccggc cgcaaggtca gcaagaagca cccgttcgaa ggcatcctgc
    24661 ccaacatggc gcggcgctgg cgcgagaccg actccaacgt ggtgcgcgaa gaactggcgc
    24721 gctaccgcag ccagcagccc tgcaccgcct gcggtggcac gcggctgcgc caggaggcgc
    24781 gccacgtgtt cgttggcgag ggcgcgcagc ggcgcgccat ctaccagatc agccatgtca
    24841 cgctgggcga ggcgcaggcc tacttcgatg cgctgcgact gacgggcgcc aaggccgaca
    24901 tcgccgagcg ggtggtgcgc gaaatcgcca gccgcctgaa gttcctcaac gatgtcggcc
    24961 tgggttacct tagcctggag cgcagcgccg acacgctgag cggcggcgag tcgcagcgca
    25021 tccgcctggc cagccagatc ggctcgggcc tgagcggcgt gatgtacgtg ctggacgaac
    25081 ccagcatcgg cctgcaccag cgcgacaacg accgcctgat cgccaccctg cagcacctgc
    25141 gcgacctggg caacacggtg ctggtggtcg agcacgacga ggacatgatg cgtgcggccg
    25201 accacatcgt cgacctcggt cccggcgccg gcgtgcacgg cgggcgcgtg atggcgcagg
    25261 gcacgttcgc gcaggtggcg gccacacccg cttcggtgac cggccagttc ctgcgcggcg
    25321 aacgggcgat cgccgtgccc agccgccgtc gacgctcaca tatcgatagc tgctcgcgca
    25381 caccggacgg gcgtcagagc ccggaaacac ttgaaacgca ccgccccacc ccgagcggcg
    25441 tgctgcgcat cgtcggcgcc cgtggccaca acctgaagga cgtcacggtg gacgttccgg
    25501 tggggctgct gacctgcgtc accggcgtgt cgggctcggg caaaagcacc ctggtcaacg
    25561 acacgctgta cacggcggcc gcgcgcgaaa ttcaccgtgc ccacgccgaa ccggcgccgc
    25621 acgaggccat cgaggggctg gcgcagttcg acaaggtgat cgcggtcgat cagtcgccca
    25681 tcggccgcac gccgcgcagc aacccggcca cctacaccgg cctgttcacc ccgatccgcg
    25741 aactgatggc cgaaaccgcc atggcgcgcg agcgcggctg gggcccgggg cgcttcagtt
    25801 tcaacgtgcc gcaggccagc ggcggcggcc gctgcgaggc ctgccagggc gacggcgtgg
    25861 tgaaggtcga aatgcacttt ctgcccgacg tctacgtgcc ctgcgacgtc tgtgccggcc
    25921 agcgctacaa ccgcgaaacg ctgcaggtgc agtggaaggg ccgcaacatc gcccaggtgc
    25981 tggacatgac ggtggaggcc gcgcacgcct tctttgccga ccagcccgcc atcgcgcgca
    26041 agctgcagac cctgctggac gtgggcctgt cctacatcac cctggggcag agcgccacca
    26101 ccttgtcggg cggcgaggcg cagcgcgtca agctggcgct ggagctgtcc aagcgcgaca
    26161 ccggccgcac cctgtacgtg ctggacgaac ccaccaccgg cctgcacttc gccgacgtcg
    26221 agctgctgct caaggtgctg caccagctgg tcgaggccgg caacacc
//
LOCUS       DAOQTZ010000030        25675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2240_length_25675_cov_12.866628, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000030 DAOQTZ010000000
VERSION     DAOQTZ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2240_length_25675_cov_12.866628"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..>1346)
                     /locus_tag="PKN51_05340"
     CDS             complement(187..>1346)
                     /locus_tag="PKN51_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNT84557.1"
                     /translation="HRYGRRMQTISGIHYNWSLPGLDNDGHFALIRNFRRHAFLLLYL
                     FGASPAVCASFVAGRAHELQPLGAHAMHLPWATSLRMGRLGYQSDAQATLAVSYNGLE
                     SYATTLEEALTRPYPAYEAVGIRNPGGDYNQLATTLLQIENEFYGTIRPKRVIRTGER
                     PLHALRERGVEYLEVRLMDLNPFEPIGIDAATARFLDLFLLHCLLTDSPPDTPQEIAA
                     LKANQHLTAARGREPGLCLDRRGESVRLSDWAAQLVGELAPIADAMDAVHGGRAHAQA
                     LVQARQRLAAPGELPSARVLQAVRDQHGSSFVAFTRQQALATRAHLLALDYPATIKQR
                     FDALAVESVQAQADIEAADRVPFETFRREYLSSRHLRPAASSSAMPGSSGA"
     assembly_gap    1347..1446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1447..1631)
                     /locus_tag="PKN51_05345"
     CDS             complement(<1447..1631)
                     /locus_tag="PKN51_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNT84558.1"
                     /translation="MDSLSDRVAALSPDRLRGIRRGIEKESLRAQPDGELALTPHPAA
                     LGSPLTHPHITTDFSES"
     gene            complement(1722..2633)
                     /locus_tag="PKN51_05350"
     CDS             complement(1722..2633)
                     /locus_tag="PKN51_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT84559.1"
                     /translation="MSKIDFNGRVAIVTGAGGGLGRLHALALAERGARVVVNDLGGAV
                     DGSGGSATAAERVVEEIRARGGQAIASGASVTDAQAMQAMVQQALDTWGRVDVLVCNA
                     GILRDKSFAKMDLADFRLVLDVHLMGAVHLVKAAWPHLVAQKYGRILMTTSSTGLYGN
                     FGQSNYGAAKLALVGLMQTLALEGAKHDIRVNCLAPTAATRMTEGLLPPDVLELLRPE
                     AVVPAMLVGVCQDAPTRAILCAGAGTYEAAHITLTQGVHLGLTADTPERLLAALDQVR
                     DRSGESVPQSGAEQGGNEVRQALAARR"
     gene            2760..3650
                     /locus_tag="PKN51_05355"
     CDS             2760..3650
                     /locus_tag="PKN51_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNT84560.1"
                     /translation="MELNHETIDTPPLDAASVVMLRDGARGLEVLLLRRHANSSVLGG
                     AYVFPGGKVDAADASADTLTLLDRAPTELHARLGEPALTPAQAAAFYVAAAREAAEES
                     GVLYLQVGADEVARHSAATCARLRAGEPFAALLAEHGALLDSDALAPWSRWITPRRPS
                     VMNKRFDTRFFLAGVPPAQQARHDDFEVTEARWLTPREALRQYREHQIDMAPPQIVSL
                     LHLLHYRDLAHAMQDARTRRPPLIEPEPIEQGDTRVLCYPGDAAHSVAARALPEPVPT
                     RVVWRGKRFEPPQGFGPFLD"
     gene            3748..5295
                     /locus_tag="PKN51_05360"
     CDS             3748..5295
                     /locus_tag="PKN51_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNT84561.1"
                     /translation="MHPAVRRQTIADLLRRTAQRLPAKLAVQCGATRWSYAEFDALVT
                     RLAAGLAGRGVAHGDRVAILARNSHGFAAMRFALARLGAVLVPINFMLKADEVAFILR
                     HAGAKLLATDSGLAELARAAAALDTQVTDFVWLPSEDPSEPVAGMASFHDLAACTAPL
                     PDTPLASTDLAQIVYTSGTESSPKGAMLTHDAVLWQYVSCCINAEVAEHDVALHALPL
                     YHCAQLDVFFGPAVYIGSTNFITSKPTPDNLLPMMAAHGITSFFAPPTVWIALLRSPL
                     FDQTDLSRLAKGYYGASIMPVEVLKEIARRLPAVRLWNLYGQTEIAPLATMLGPDDQL
                     RKPGSAGRAVLNVETRVVDDALRDVAVGEVGEIVHRSPHLMLGYFHDDERTAAAFEGE
                     WFHSGDLGTIDAEGYITVVDRKKDMIKTGGENVASREVEEMIYRLPEVSEVAVIGLPD
                     AKWVEAVTAVIVLKAGQELTEAQVIAHCNAHMAAFKAPKRVIFTDQLPKNPSGKLLKR
                     QLRQTFV"
     gene            complement(5436..6407)
                     /locus_tag="PKN51_05365"
     CDS             complement(5436..6407)
                     /locus_tag="PKN51_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HNT84562.1"
                     /translation="MATSLLALLDDIATVLDDVAVMSKIAARKSAAMADDVAVLTKVS
                     AQKTAGVLGDDLALNAQQVTGVSAHREIPVVWAVARGSFVNKAILVPVALLVSAFAPW
                     AVTPLLMLGGAFLCFEGCEKLLHKLLHTRAEDQAHEAELEAALADPRVDLAALEKDKI
                     KGAVRTDFILSAEIIAITLGAVAQADFWTQVAVLVGIAILMTVGVYGLVGGIVKLDDL
                     GLWLSRRAGAGAQALGRGILALAPWLMKGLSVAGTAAMFLVGGGIIVHGVPALHHLLE
                     GWAAGLGGVLRVLADMGFNAAVGMLVGAVVVAAVTLFQRLRGTPRTA"
     gene            6736..7215
                     /locus_tag="PKN51_05370"
     CDS             6736..7215
                     /locus_tag="PKN51_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNT84563.1"
                     /translation="MPLLLTRIAGRALACVGLMCAAAAMAQAPEAPAGDGRQGTFKTV
                     RGDVTVVRDNTRVAAIVGAGVRSTDRILSGAESAAAVTLLDGTVLTIGPDSALDLSQY
                     QFNPTTQEGSVLVNLMRGSLRMVTGLIAKLKPEQVKVTTPTSVIGVRGTDFIVEENP"
     gene            7212..7796
                     /locus_tag="PKN51_05375"
     CDS             7212..7796
                     /locus_tag="PKN51_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT84564.1"
                     /translation="MRLAPLLTAVALLAAGCAPTTRVTLLPQADHPQAAVEVTARQRS
                     TRLAQPYASAAVRQQGPELEQLDAATVHKRYPALLAVMPPPAERFELKFITGGTRLTP
                     ESTAELSDVLASAIARAGGEIVVIGHTDRVGTLEANDALSLRRAEAVRALIVERGFEA
                     ARVRAVGRGEREPQVPTEDDVDEPRNRRVEIVVR"
     gene            7911..8555
                     /locus_tag="PKN51_05380"
     CDS             7911..8555
                     /locus_tag="PKN51_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNT84565.1"
                     /translation="MAQRTQACTACHGEQGRAGPDGYYPRLAGKPAGYLYHQLLHFRD
                     GRRHYGLMTRMVDVLSDAYLMDIARHFAALQVPYPPPAPPASPPPPAVLERGRALALQ
                     GDAAQGVPACVQCHGRTLTGALPDVPGLLGLPADYLSAQLGGWRTGQRRAHAPDCMAR
                     IVQRLSDRDAHAVVSWLASQPVPADPRPAPARPPLPAEWADLRCGRAPEPEVAR"
     gene            8552..9832
                     /locus_tag="PKN51_05385"
     CDS             8552..9832
                     /locus_tag="PKN51_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNT84566.1"
                     /translation="MSALRRLLLGLAGLALLGAGLLWQINFNDGVDTRSSAPVASDAA
                     TRARGAYLARAGNCLACHTARGGPAAAGGRPIATPFGTVYSSNLTPDARAGIGGWNAA
                     TFWRALHHGRSADGRLLNPVFPYTHTTGLTRADSDALFSYFLGLPPSATAVPAHELRW
                     PFGTQAALALWRALYFRPGSFQPDPARSEQLNRGAYLVQAVAHCSACHTPRDRLGGAD
                     WQSLSGGTVPGTGWYAPSLTARDEGAVGHWPAEQIVQLLATGAAPQGVASGPMAEVVY
                     QGTQHLSGADLQAVAAYLQHLPPQPGRQDAPAPRQRPSAAGERLYGQHCAACHGEDGQ
                     GVPGAYPPLSGNRSVTMANTQNLLQIVLFGGFGPATAGRPQPFGMPPFALTLSDAEIA
                     AVLSYIRGAWGNDAGEVAPLDVVRMRHVNHSGLP"
     gene            9978..11132
                     /locus_tag="PKN51_05390"
     CDS             9978..11132
                     /locus_tag="PKN51_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84567.1"
                     /translation="MALNWLMSKRARPQTEGDGAQRPDGRAAEAEANADDQELRWPEV
                     VRHFGQEVAMALNTAADQLERLSRLEPSLVGSLQTVTDAVERARQAGMAAQQVLRLTE
                     DPPVQHRELLGLDDVVRAVLTTRAEWLQRHQVGVRHGLAQTQVYADSSMLYMLVDELL
                     LWAAQMAPQIAIMVDRSSRSGRPRLSVLAWCDPAQVPEAAWRGMRWMLWHQLARSLGA
                     SARVDTQTDHVRVTLSLSAVTEEQLATGIEESVGPSSVSAVIQGCRVLIVSGNAQRRA
                     QSLQALTGYGLILDSAGDMTEAERLATRHLPDAVVYDGSVNPSAVAGLRESLEARAGT
                     PAAFIEIHDDDSASDFRTSTVGSVSTGHIAAGSLNQALGPALVFELCKVM"
     gene            11225..11914
                     /locus_tag="PKN51_05395"
     CDS             11225..11914
                     /locus_tag="PKN51_05395"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT84568.1"
                     /translation="MSAQPRHPASAAATTADPPLQVRYEDAHLIALDKPAGLLTVPGR
                     GTDKSDCLSRRAQARWPDALVVHRLDQATSGLLLLARGPAMQRALSQAFAQRQVHKRY
                     EALVHGRLTLPADATDGWARIDLPLIVDWPQRPRSKVDHVAGKPSQTRWRPLAHDAQT
                     RRTRLALEPLTGRSHQLRVHLLAIGHPIVGDTLYGPPDGAPRLMLHACALRLTHPVTG
                     QPLALDSAAPF"
     gene            11989..12579
                     /locus_tag="PKN51_05400"
     CDS             11989..12579
                     /locus_tag="PKN51_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HNT84569.1"
                     /translation="MTRRNLQGLGLALSLALGGPWLSGCTITSTPQSQAAPAATRAAI
                     DAAVNETLSRLYHLVPGTQDMVSRAAGVLVFPSVLGGSFVVGAEHGKGALRVGGQTTS
                     YYSTTGASIGFQAGGQSRSVIYVFNTREALQSFRASNGWTADADATVAIGNVGANGRV
                     DSHTVQQPIASFVLTNVGLEAGVSVGGSRIVPIALP"
     gene            12643..13422
                     /locus_tag="PKN51_05405"
     CDS             12643..13422
                     /locus_tag="PKN51_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT84570.1"
                     /translation="MNRKPTRQLYVLTGASRGMGRAMAQQLLAPGHQLLTLSRRPDEA
                     LASQAGAAGATLTQWAVDLGDAAPVAARLADWLRAQDGKALTSATLINNAGAIPAIVP
                     LRDADPPALAAALRLGLEAPMQLCAAFLSATRDWPGTRRVLNISSGLGRRAMASQAAY
                     CAAKAGMDHFTRCLALEEARLPQGARVCSLAPGVIDTDMQVQLRGADAAAFPDRDSFE
                     QLKRGGALSSPADAARRVLAYLARPDFGQHPVADVREVLGA"
     gene            13647..14831
                     /locus_tag="PKN51_05410"
     CDS             13647..14831
                     /locus_tag="PKN51_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HNT84571.1"
                     /translation="MSREVVVVSGVRTAIGTFGGSLKDTPTVDLGALVVKEALARANV
                     DGKDVGHVVFGHVVNTEPRDMYLSRVAAINGGCAKETPAMNVNRLCGSGLQAIVSASQ
                     AILLGDCDIAIGGGAENMSRAPYASLATRFGARMGDAKMVDMMVGALHDPFHTIHMGV
                     TAENVAVEHGITREAQDALALESHRRAEVAWAEGRFKDQIVPVMLKTRKGEVAFDKDE
                     HFRPGAKLEDFQKMKPVFLKENGTVTAGNASGINDAAAAVVLMEAGVAKARGAKPLAR
                     LVGYAHAGVEPKTMGIGPVPATQMLLKKLGLTIDQMDVIEANEAFAAQACAVTKGLGA
                     DPAKVNPNGSGISLGHPIGATGALITVKALYELQRIQGRYALVTMCIGGGQGIAAIFE
                     RM"
     gene            14950..15369
                     /locus_tag="PKN51_05415"
     CDS             14950..15369
                     /locus_tag="PKN51_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84572.1"
                     /translation="MRLDPFRRVDDTPFSARRTDIERARGQPLRVEPNAVGLTALDYG
                     DVVFRLQDSSGRLEEVTRRAPVLYLLRGDSVVDVPFAALAGYVRTHDARAFERGGFLV
                     SPRLGLAFVPTEPDWVTALAAHCVDAWRRLGNDAAQE"
     gene            complement(15380..15763)
                     /locus_tag="PKN51_05420"
     CDS             complement(15380..15763)
                     /locus_tag="PKN51_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84573.1"
                     /translation="MTTLTLTTLYTGPDGRARFRDDRLALPDGTPAARLSALQASGGC
                     QFRHSPVGFRSDFHCTTTPQWLVVLQGRMEIGLQDGSTRVFGPGQCFYSNDTLPPGAA
                     FDPSVHGHCSRQLGDEPLVTLFVRA"
     gene            15887..16720
                     /locus_tag="PKN51_05425"
     CDS             15887..16720
                     /locus_tag="PKN51_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84574.1"
                     /translation="MKALATLLGALALSGGVLLASPAQAAGQWFDVHVIDRDSGSRLP
                     LYRHDGQWWVAGRPGARYAIELRNTSGTRLLGVVSVDGVNVISGETADWQQSGYVLAP
                     HQRAQITGWRKSEAEVAAFHFTALPHAYATRTGRPEHVGVIGVAVFRERTPAPPPPEW
                     SEHRRAPAAGEAPPSPAAPAAAAADSESGAASRREAQAAAPRLGTGHGAREPAWVTRT
                     RFERRSSQPDEVIAIRYDSRENLLAMGVIPQHPRQAQAPRPFPDAPPGPGYTPDPPAW
                     R"
     gene            16772..17371
                     /locus_tag="PKN51_05430"
     CDS             16772..17371
                     /locus_tag="PKN51_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT84575.1"
                     /translation="MPAPVASADEPLMRAYAAGDASAFETLYDRHQLRLWRYLLRSVG
                     DAATADELAQDVWLRVAREAPRYTPSAAAPGRPPARFTTWLFTLAHHRVVDHLRRQRP
                     TLSLDEPLHDELTLADTLAAPSGFGPLRQIETRQQAVQLLAALDALPPEQRTAFLLQA
                     EGEMSVAEIASTTGVGLETARSRLRYARAALRRKLEHLA"
     gene            17368..18648
                     /locus_tag="PKN51_05435"
     CDS             17368..18648
                     /locus_tag="PKN51_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT84576.1"
                     /translation="MNAPPALPRPTDDDLVERYHEAAALEDRGPAPELRAAVLAQARM
                     LAWRHPEAADDGPSNATESIAGGDDPASAAAVFDAEAGSPRRPRGAANDRRWLISTAA
                     SVAVLGLAGLLALQFERSPQGLREAAPQAGPTALVDAAPPPAPPATAAAPLAPTPAAA
                     TAAPPAAAPKPETIQPAPAPAAPHPPETTPPATPHLPPPAADAATPATAARAPDPRAA
                     RPKAEPRAREPAARPDLRRPAADARPDRPPPTAPARAEHPEAEAGAPAPRPPQASEAP
                     SPVPPDRAAARTGTASPNGTASPNGIASPNGTASPNGIGAEPTAAPAAPAAPAPVLTP
                     PQRLLAAAAAGQADAARAALEQGAAVNATDSAGRTALMLTARRGNVGIARTLVDAGAD
                     PRQPDRQGLTAIDHARRAGHEALARWLEERARPR"
     gene            18725..19510
                     /locus_tag="PKN51_05440"
     CDS             18725..19510
                     /locus_tag="PKN51_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT84577.1"
                     /translation="MTATSHPPICFGLQGRVVIVTGAAQGIGEACARRFAQEGAQVVL
                     ADIADAPGQALAQALGASYVHCDVGDKAQVDALVAQVLQAHGRIDVLVNNAGIFKAAP
                     FLQVTEDDFDAVLRVNLKGAFLVGQAVARPMAEAGRGSIVNMSSVNSVLAIPDIASYN
                     VSKGGINQLTRAMALALADRGVRVNAVGPGTIATELAAQAVLTSEDARHRIMMRTPMK
                     RLGEPREVADVVAFLASDAASYITGEIVMVDGGRMALNYTVPV"
     gene            19516..20340
                     /locus_tag="PKN51_05445"
     CDS             19516..20340
                     /locus_tag="PKN51_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT84578.1"
                     /translation="MLIAHLTDTHLGIDSGALGGRLDPAAALRRALARVRALEPAPAV
                     LLLTGDLAEAGRAADYTLLAELLKQELPAHAGQAPRVLCIIGNHDHRGHARQTLGALM
                     PIAPEAPPPLACVRAEVGGLQFIGLDTVLPGHPHGALDAPQLDWLARQLRDCAGQPVA
                     LFMHHPPLTSGIEAMDRCGLRRGRAELARLVAAHGGVQFIACGHLHRPIAGVLGGAPV
                     LVAPSTSHQLELDLRPGAPLALRLEPPMLGLYRWTAQDGVSCHFGHVQTFEGPYPI"
     gene            20571..22103
                     /locus_tag="PKN51_05450"
     CDS             20571..22103
                     /locus_tag="PKN51_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT84579.1"
                     /translation="MSLAHLLLRQAQRQPQAPAILDGAQPHASYLQWAARSAGLARHL
                     RDGGLQPGDRVLLFAHNHPRYLELLWGLWWAGLVAVPVNAKLHPREVEWIAADAGAQL
                     AFVTRDVAPDALAGVAHQVDLESMEADQLLAPVAEPAAVPITERAPDDIAWLFYTSGT
                     TGRPKGVMLTQRNLMTMGLGYFADVDPIARGDAIVYAAPISHGAGIYAIPHLMAGARH
                     VVPASRGVDAAELFALGRAVGPLSMFAAPTIVARLVDHAAQQGLGPELCARSFKTIVY
                     GGAPMYAADIQRALSVMGPRFVQIYGQGETPMVGTVLSRADLKDRAHPRHAERIASVG
                     VAQTPVRVRVTDAQGQDLPLGEVGEVLIQGDSVMAGYWGNPQATAAAIRNGWLFTGDM
                     GALDEGGYLTLKDRSKDLIISGGSNIYPREVEEALLTAAGVAEVAVVGQPDPEWGENV
                     VAFVVAQPGAAPTQEALDAHCLQRIARFKRPKRYVFVPELPKNNYGKVLKTELRRWLA
                     SA"
     gene            complement(22262..23299)
                     /locus_tag="PKN51_05455"
     CDS             complement(22262..23299)
                     /locus_tag="PKN51_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT84580.1"
                     /translation="MSSKLNKLMAAAALSLLAGTAAQAQTVLKVHHFLPSGSSSQVKI
                     IAPWCAKIEKESGGELKCQIYPAMQLGGTPPQLLDQVRDGVADIVWSVPTYSAGRYTK
                     SEVFELPFMAINAKQGSQALWEYVQKNALDEYKGIKPLFMHTTEGYVLHSNKPILKLE
                     DMKGKKIRTATRISARMMSAMGGTPVQMPLPQVPDALSKGVVDGVLVPWEALPATKLH
                     EIVKYHLDAPVGSPRFANTIFIFGMNQAKYDSLSPKLKKVIDDNSGLAASAWAGEEGF
                     DNIVGPYSKLARDRGNTIAVIEPAELARWEKATADVDEIWLKDAAAKGYDGKKLIEEA
                     KALLKKYAPAK"
     gene            complement(23332..24654)
                     /locus_tag="PKN51_05460"
     CDS             complement(23332..24654)
                     /locus_tag="PKN51_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNT84581.1"
                     /translation="MSTLTIGLLLFVAMLLLMAIRVPIAVAMFVPGAVGYVILSGWDP
                     LLAHLRGAVYGRVSVYDLSVIPLFMLMGGFAVHGGLSKALFDFANALLGRFKGGMAMA
                     GILACAAFGAISGSTVATTATIAQVAYPEMRRISYSGRLATAALATGGTMGVLLPPSV
                     TLMVYAILTEQNITKTFLAAYVPAMLAALGYLVVIAIYVRLHPTHAPEARAASASEVA
                     QSAAGVWPIALIFVAVFGGIYGGIFTPTEGAAIGATLTFAITLINRQLTLKKAIESIR
                     TTAVTSGMIFMIFLGADMMNASLALSQLPNQLAELIAHAAIPPVLVMAVIMLFYVALG
                     CVMDEMSMILLTVPVVFPVIMGMDFFGLDASDKALWFGILILTVCAIGMIFPPVGLNV
                     YVMNGLAKDVPMVETYKGVFIFLAWDFIRLGLLIMFPGITLWLVRLLT"
     gene            complement(24651..25178)
                     /locus_tag="PKN51_05465"
     CDS             complement(24651..25178)
                     /locus_tag="PKN51_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT84582.1"
                     /translation="MTADNRMREGPVGRLLHVLCDICAVVGGAVLIAMAIMTVVSVIG
                     RTFFDSPILGDVELVQLGLAVCVATFLPYTQFRSANIIVDFFTTRASARSQRWMDGLG
                     CLLYAGMTALIAWRVYAGGVMSQENQEASMLMNLPLWIPYMLMVPGLVLCTLVGLYQA
                     VIYWTQPAAAEGDAA"
BASE COUNT         3828 a         9462 c         8788 g         3497 t
ORIGIN      
        1 caccatgcct gcggccacgc gccgcgcagc tggccccttg gcgactcgct ataagcaccc
       61 gaaggcgcga aacactgcac ttgggcccga cggcggcgcc ggcctggaca cgacaggcac
      121 gcgtgcccgt cacggccggc aaccggattg ggaccggacg tctcgaggat ggggggccgg
      181 cagcggtcag gcgccggagc tgcccggcat ggcggacgag cttgcggcgg gccgcagatg
      241 ccgtgacgac aggtattcgc gccggaaggt ttcgaacggc acgcggtcgg ccgcttcgat
      301 gtcggcctgg gcctgcaccg actcgaccgc cagggcgtca aagcgctgct tgatggtggc
      361 cgggtagtcc agcgccagca ggtgcgcgcg ggtggcgagc gcctgctgcc gcgtgaaggc
      421 cacgaaactg ctgccgtgct ggtcgcgcac cgcctgcagc acgcgcgccg agggcagctc
      481 gccaggcgcc gccaggcgct ggcgggcctg caccagtgcc tgggcgtgcg cacggccacc
      541 atgcacggcg tccatggcgt cggcgatggg tgccagctcg ccgacgagct gcgcggccca
      601 gtcgctcagc cgcaccgact cgccacggcg atccaggcac aggccgggct cgcgcccgcg
      661 cgcggcggtc aggtgctggt tggccttgag cgcggcgatt tcctgcggtg tgtcgggcgg
      721 gctgtcggtg agcaggcagt gcagcaggaa gaggtcgaga aagcgcgccg tcgccgcgtc
      781 gatgccgatc ggctcgaacg ggttcaggtc catcaggcgc acttcgaggt attcgacgcc
      841 gcgctcgcgc agcgcatgca gcgggcgctc gccggtgcgg atcacgcgct tggggcggat
      901 ggtgccgtag aactcgtttt cgatctgcag cagcgtggtg gccagctggt tgtagtcgcc
      961 gcccgggttg cggatgccga ccgcttcgta ggccgggtag ggccgtgtca gcgcctcttc
     1021 cagggtggtg gcgtagcttt ccaggccgtt gtagctgacc gccagcgtgg cctgggcatc
     1081 gctctggtag cccagccgcc ccatgcgcag cgaagtcgcc cagggcaggt gcatggcgtg
     1141 cgcgcccagc ggctgcagct cgtgcgcgcg gccggccacg aagctggcgc acaccgccgg
     1201 cgaggcgccg aacaggtaca gcagcaggaa ggcgtggcgg cggaagttgc ggatcagcgc
     1261 gaagtggccg tcgttgtcca agccgggcag cgaccagttg tagtggatgc ccgagatggt
     1321 ctgcatgcgc cggccgtagc ggtggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnntgcg attcgctgaa gtcggtggtg atgtgcgggt gcgtcagcgg cgagcccagc
     1501 gccgccgggt gcggggtcag cgccagttcg ccgtcgggct gggcgcgcag gctttctttc
     1561 tcgatgccgc ggcggatacc tcgcagtcgg tcgggcgaca gcgccgccac gcgatcgctc
     1621 agcgaatcca tgtgctcttg tccttgtctt gccggggtgg cgaaggtatc acaggcccgc
     1681 cattcccgtc ttcgggcacg ggaatccgcc gcgccgggcg ttcagcgccg tgcggccagc
     1741 gcctggcgca cctcgttgcc gccctgttcg gcgccgcttt gcggcacgct ctcgccgctg
     1801 cgatcgcgca cctgatccag cgcggccagc aggcgctcgg gtgtgtcggc ggtcaggccc
     1861 agatgcacgc cctgggtcag ggtgatgtgc gcggcctcgt aggtgccggc gccggcgcac
     1921 aggatggcgc gcgtgggcgc gtcctggcac acgcccacca gcatggccgg caccaccgcc
     1981 tcgggccgca gcagctccag cacgtcgggc ggcagcaggc cctcggtcat gcgggtggcc
     2041 gccgtgggcg ccaggcagtt gacgcggatg tcgtgcttgg cgccttccag cgccagcgtc
     2101 tgcatcaggc cgaccagcgc cagcttggcg gcgccgtagt tgctctggcc gaagttgccg
     2161 tagagaccgg tggacgaggt ggtcatcagg atgcgcccgt atttctgcgc caccaggtgc
     2221 ggccaggccg ccttgaccag gtgcaccgcg cccatcaggt gcacgtccag caccaggcgg
     2281 aagtcggcca gatccatctt ggcaaagctc ttgtcgcgca ggatgccggc gttgcacacc
     2341 agcacatcga cccggcccca ggtatccagc gcctgctgca ccatggcctg catggcctgg
     2401 gcgtcggtca ccgaagcgcc gctggcgatc gcctggccgc cgcgggcgcg gatttcctcg
     2461 accacgcgct cggccgcggt cgccgagccg ccgctgccgt ccaccgcgcc gcccaggtcg
     2521 ttgaccacca ccctggcgcc gcgctcggcc agcgccagcg cgtgcaggcg ccccaggccg
     2581 ccgccggcgc cggtgacgat ggccacgcgg ccgttgaagt cgatcttgct catgctgtgc
     2641 tcactcctgt tgaaagaatc ccgtcgatgc cgactgtaac caaccggcca ggccgtatgc
     2701 gcccgtgggc ggacgcaggt gacagcggcg ccccgcggcc ccggttcaca atgcgcgcca
     2761 tggaactgaa ccacgaaacc atcgacaccc cgccgctcga cgcagccagc gtggtgatgc
     2821 tgcgcgacgg cgcccgggga ctggaagtgc tgctgctgcg ccgccacgcc aactcgtcgg
     2881 tgctgggcgg cgcctacgtg tttcccggcg gcaaggtgga cgcggccgat gccagcgccg
     2941 acacgctgac cctgctggac cgcgcgccga ccgagctgca tgcgcgcctg ggcgagccgg
     3001 cgctgacgcc ggcgcaggcg gcggcgttct acgtcgctgc ggcacgcgag gcggccgagg
     3061 aatcgggcgt gctgtacctg caggtgggcg ccgacgaggt ggcccgccac agcgccgcca
     3121 cctgcgcccg cctgcgcgcc ggcgaaccgt ttgccgccct gctggccgag cacggcgcgc
     3181 tgctggacag cgacgccctc gccccctggt cgcgctggat cacgccgcgc cggccctcgg
     3241 tgatgaacaa gcgcttcgac acgcgctttt tcctggccgg cgtgccgccg gcgcagcagg
     3301 cgcgccacga cgacttcgag gtcacggaag cgcgctggct gacgccacgc gaggcgctgc
     3361 ggcaataccg cgagcaccag atcgacatgg cgccgccgca gatcgtcagc ctgctgcacc
     3421 tgctgcacta ccgcgacctg gcgcatgcca tgcaggatgc gcgcacgcgc cgcccaccgc
     3481 tgatcgagcc ggaaccgatc gagcagggcg acacgcgcgt gctgtgctac cccggcgacg
     3541 cggcgcacag cgtcgcggcg cgcgcgctgc ccgagccggt gcccacgcgc gtggtgtggc
     3601 gaggcaagcg tttcgagccg ccacagggtt tcgggccgtt cctggactga accaagatac
     3661 cttgagcaac tatcaaacta atagctgctg gcgattgccc cacctgcctc agcgcccgaa
     3721 atgccatccc aaaccggaga cctcccgatg catcccgccg tccgccgcca gaccatcgcc
     3781 gatctgctgc gccgcaccgc ccagcgcctg cccgccaagc tggcggtgca gtgcggcgcc
     3841 acgcgctgga gctatgccga gttcgacgcc ctggtcaccc gcctggccgc cggcctggcc
     3901 ggccgcgggg tggcgcatgg cgaccgggtg gccatcctgg cgcgcaattc gcacggcttt
     3961 gccgccatgc gctttgccct ggcgcgcctg ggcgcggtgc tggtgccgat caacttcatg
     4021 ctgaaggccg acgaggtggc gttcatcctg cgccacgccg gcgccaaact gctggccacc
     4081 gacagcggcc tggccgagct ggcgcgcgcc gccgccgcgc tggacacgca ggtgacggac
     4141 ttcgtgtggc tgccttcgga agacccgagc gaaccggtgg cgggcatggc cagctttcac
     4201 gacctggccg cctgcaccgc gccgctgccc gacacgccgc ttgccagcac cgacctggcg
     4261 cagatcgtct acaccagcgg caccgagtcc agccccaagg gcgccatgct gacgcacgac
     4321 gccgtgctgt ggcagtacgt cagctgctgc atcaacgccg aggtggccga gcacgacgtg
     4381 gcgctgcacg cgctgccgct gtaccactgc gcccagctcg acgtgttttt cggccccgcc
     4441 gtctacatcg gctcgaccaa cttcatcacc agcaagccca cgcccgacaa cctgctgccc
     4501 atgatggcgg cgcacggcat caccagcttc ttcgccccgc ccacggtgtg gatcgcgctg
     4561 ctgcgctcgc cgctgttcga tcagaccgat ctgagccggc tggccaaggg ctactacggc
     4621 gcttccatca tgccggtcga ggtgctcaag gaaatcgcgc gccgcctgcc cgccgtgcgc
     4681 ctgtggaacc tgtacggcca gaccgaaatc gcgccgctgg ccaccatgct gggccccgac
     4741 gaccagctgc gcaaacccgg atcggccggc cgcgcggtgc tgaacgtgga gacgcgggtg
     4801 gtcgacgacg ccctgcgcga cgtggccgtg ggcgaggtgg gcgagatcgt gcaccgctcg
     4861 ccgcacctga tgctgggcta ctttcacgac gacgagcgca cggcggcggc cttcgagggc
     4921 gagtggtttc actcgggcga tctgggcacc atcgacgccg agggctacat caccgtggtc
     4981 gatcgcaaga aggacatgat caagaccgga ggcgagaacg tcgccagccg cgaggtcgag
     5041 gaaatgatct accgcctgcc cgaagtcagc gaggtggcgg tgatcggcct gcccgacgcc
     5101 aagtgggtcg aggcggtgac ggcggtgatc gtcctcaagg ccgggcagga gctgaccgaa
     5161 gcgcaggtga tcgcccactg caacgcacac atggcggcct tcaaggcgcc caagcgcgtg
     5221 atcttcaccg accagttgcc caagaacccc agcggcaagc tgctcaagcg ccagttgcgc
     5281 cagaccttcg tctgaccgcc gcgccccgcc ggcgagtgtg cgacagcgct gcccgtggtg
     5341 cgcgccgacc gggcgccggg gcctccagac cttcaagtct ttctggcccc cggcgcgcac
     5401 ccatcaagcg cgagcagcta tcgaattcat agtattcaag cggttcgcgg ggtgccgcgc
     5461 aggcgctgaa acagcgtcac cgccgccacc accaccgcgc cgaccagcat gcccacggcg
     5521 gcgttgaagc ccatgtcggc cagcacgcgc aacacgccgc ccaggccagc ggcccagcct
     5581 tccagcaggt ggtgcagcgc cggcacgcca tgcacgatga tgccgccgcc caccaggaac
     5641 atggcggccg tgccggccac cgacagcccc ttcatcagcc agggcgccag cgccaggatg
     5701 ccgcggccca gcgcctgcgc gccggcgcct gcccgccggc tcagccacag gcccaggtcg
     5761 tccagcttga cgatgccgcc caccaggcca tacacgccca ccgtcatcag gatcgcgata
     5821 ccgaccagca cggccacctg ggtccagaaa tcggcctgcg ccacggcgcc cagggtgatg
     5881 gcgatgatct cggccgagag gatgaagtcg gtccgcacgg cgcccttgat cttgtctttt
     5941 tccagcgccg ccagatcgac ccgcgggtcg gccagcgccg cctccagctc ggcctcgtgg
     6001 gcctggtcct cagcacgggt atgcagcagc ttgtgcagca gcttctcgca accctcgaag
     6061 cacagaaagg cgccacccag catcaacagt ggcgtcaccg cccagggcgc aaaggcgctg
     6121 acgagcaggg ccaccggcac caggatggcc ttgttgacga acgaccctct ggccaccgcc
     6181 cacaccaccg ggatctcgcg gtgggcgctg acgccggtca cctgctgggc gttcagggcc
     6241 aggtcgtcgc ccagcacgcc ggcggtcttc tgcgccgaca ccttggtcag caccgccacg
     6301 tcgtcggcca tggcggcgct cttgcgcgca gcgatcttgg acatgacggc gacgtcgtcg
     6361 agcacggtgg cgatgtcgtc gagcagggcg agcaggctgg tggccatgaa ggctttccgg
     6421 gaaaaggcga agaatgcggg gtcggtacgt cgaacccggg gccggcaggc catcgggcct
     6481 ggcacgccgc ccgagcgcca acccctcggc aggcggcgat tatctccgcg ccgcgtgccc
     6541 gccacccggg caagtcggcg cctgaaaacc gtggggtacc attgcaggtt ggccctcggg
     6601 ccgcaccaca gccttcattg attcggcctt tggatgtctt gaacatcttt cgcgctgagc
     6661 tcgtccgagc ccttcgacaa gctcaggttg ggcggttcag atttcaggat tccgaaaccc
     6721 taggagaaca cgcccatgcc cctgctcctg acccgcatcg ccggccgcgc cctggcctgc
     6781 gtcggtctga tgtgcgcggc cgccgccatg gcccaggcgc ccgaggcgcc ggccggtgac
     6841 gggcgccagg gcaccttcaa gacggtgcgc ggcgacgtca ccgtggtgcg cgacaacacc
     6901 cgcgtggcgg ccatcgtggg tgcaggcgtg cgatcgaccg accgcatcct gagcggcgcc
     6961 gaaagcgccg ccgccgtcac gctgctcgac ggcacggtgc tgaccatcgg gcccgacagc
     7021 gcgctggacc tgagccagta ccagttcaac cccaccacgc aggaaggcag cgtgctggtc
     7081 aacctgatgc gcggctcgct gcgcatggtc accgggctga tcgccaagct caagcccgag
     7141 caggtcaagg tcaccacgcc gaccagcgtc atcggcgtgc gcggcaccga cttcatcgtg
     7201 gaggaaaacc catgaggctg gcccccttgc tgacggccgt ggccctgctg gcggccggtt
     7261 gcgcaccgac cacccgcgtc accctgctgc cgcaggccga ccacccgcag gccgcggtcg
     7321 aggtcacggc gcgccagcgc agcacgcggc tggcgcagcc ctacgcctcg gcggcggtgc
     7381 gccagcaggg cccggagctg gagcaactcg acgccgccac ggtgcacaag cgctaccctg
     7441 ccctgctggc cgtgatgcca ccgccggccg agcgtttcga gctgaagttc atcaccggtg
     7501 gcactcgact gacgcccgaa tccaccgccg aactgagcga tgtgctggcg agcgccatcg
     7561 cgcgcgcggg cggtgagatc gtcgtcatcg gtcacaccga ccgggtcggc acgctggagg
     7621 cgaacgacgc gctttcgctg cgccgcgccg aagcggtgcg cgccctgatc gtcgagcgcg
     7681 gcttcgaggc ggcgcgtgtg cgcgccgtcg ggcgcggcga gcgcgaaccg caggtgccca
     7741 ccgaggacga cgtcgacgag ccgcgcaacc ggcgcgtcga gatcgtggtg cgctgagcat
     7801 caccgccggc cacccatcct tcgcgcatgc gcccgaagtc gcgaacctgg ctgttggccg
     7861 gcgcgctgct gctggcccac acggtgcagg cggcgccgtt ccgtgacgac atggcgcagc
     7921 gcacccaggc ctgcaccgcc tgccacggcg agcagggccg cgccggcccc gacggctact
     7981 acccgcgcct ggcgggcaag ccggcaggct acctgtacca ccaactgctc catttccgtg
     8041 atggaaggcg ccactacggc ctgatgacgc gcatggtgga cgtgctgagc gacgcctacc
     8101 tgatggacat cgcgcgccac ttcgccgccc tgcaggtgcc ctatccgccc ccggcaccgc
     8161 cggccagccc gccgccgccg gcggtgctcg aacgcggccg cgcgctggcg ctgcagggcg
     8221 atgccgccca gggcgtgccg gcctgcgtgc aatgccacgg ccggacactg accggcgccc
     8281 tgcccgatgt gcccggcctg ctcgggctgc cggccgacta cctgagcgcg cagctgggcg
     8341 gctggcgcac cggccagcgg cgcgcccacg cgcccgactg catggcccgc atcgtgcagc
     8401 gcctgtccga tcgcgatgcc cacgccgtgg tcagctggct ggccagccag ccggtgccgg
     8461 ccgacccccg ccccgccccc gcacgcccgc ccctgcctgc cgagtgggcc gacctgcgct
     8521 gtggccgcgc ccccgagcca gaggtcgcca ggtgagcgcg ctgcgccgcc tgctgctggg
     8581 gctggccggg ctggcgctgc tgggggcggg cctgctgtgg cagatcaact tcaacgacgg
     8641 tgtcgacacc aggtccagcg caccggtcgc cagcgacgcg gccacgcgcg cgcgcggcgc
     8701 ctacctggcc cgggcaggca actgcctggc ctgccacacg gcgcgcggcg gcccggcggc
     8761 ggccggcggc cggcccatcg ccacgccctt cggcaccgtc tacagcagca acctgacgcc
     8821 cgatgcgcgc gccggcattg gcggctggaa cgccgccacc ttctggcgcg cgctgcacca
     8881 tggccgctcg gccgacgggc ggctgctcaa cccggtgttc ccctacaccc acaccaccgg
     8941 gctcacgcgc gccgacagcg acgccctgtt cagctacttc ctcggcctgc cgcccagcgc
     9001 caccgcggtg ccggcccacg agctgcgctg gcccttcggc acccaggcgg cgctggcgct
     9061 gtggcgcgcg ctgtacttcc ggcccggcag ctttcagccc gacccggccc gcagtgagca
     9121 gctcaaccgc ggcgcctacc tggtgcaggc ggtggctcac tgctcggcct gccacacccc
     9181 gcgcgaccgc ctgggcggcg ccgactggca gagcctgagc ggtggcacgg tgcccggcac
     9241 cggctggtac gcgccctcgc tgacggcacg cgacgaaggc gccgtggggc actggccggc
     9301 cgagcagatc gtccagctgc tggccaccgg cgccgcccca caaggggtcg ccagcgggcc
     9361 gatggccgaa gtggtctatc agggcacgca gcacctgagc ggcgccgatc tgcaggccgt
     9421 ggcggcctac ctgcagcacc tgccgccaca gccgggccgg caggacgcgc cggcgccacg
     9481 ccagcgcccc agcgcggccg gcgagcggct gtacgggcag cactgcgcgg cctgccatgg
     9541 cgaagacggc cagggcgtgc ctggcgccta tcccccgctg tccggcaacc gcagcgtcac
     9601 catggccaac acccagaacc tgctgcagat cgtgctgttc ggcggtttcg gaccggccac
     9661 cgcgggccgc ccgcagccct tcggcatgcc gccgttcgcc ctgaccctga gcgatgccga
     9721 gatcgcggcg gtgctgagct acattcgcgg cgcctggggc aatgatgccg gcgaagtggc
     9781 cccactcgac gtggtgcgca tgcgccacgt caaccattcg ggtcttccgt gacattggtt
     9841 acgcttgaac cgcaacggca acgatttgtg acggttcgcg ccctatactg cggcgtcagc
     9901 cgtgacgccc ccacagcacc atccggtgcc tgctcaggtc gggttgcggc gcaaacactg
     9961 gggaggaacg aaccaccatg gcgctgaact ggctcatgtc caagcgggcc cgtccgcaaa
    10021 ccgagggcga cggcgcccag cggcccgacg gccgtgcggc cgaggctgag gccaacgccg
    10081 acgatcagga gctgcgctgg cccgaggtgg tgcgccattt cggtcaggaa gtggcgatgg
    10141 cgctgaacac cgccgccgac cagctggaac gcctgagccg gctggaaccc agcctggtgg
    10201 gcagcctgca aaccgtgacc gacgccgtcg agcgtgcacg ccaggccggc atggccgccc
    10261 agcaagtgct gcgcctgacc gaagacccgc cggtgcagca tcgcgagttg ctgggcctgg
    10321 acgacgtggt gcgcgccgtg ctgaccacgc gcgccgagtg gctgcagcgt caccaggtcg
    10381 gcgtgcggca tggcctggcg cagactcagg tctatgccga ctccagcatg ctgtacatgc
    10441 tggtcgacga actgctgctg tgggcagccc agatggcacc ccagatcgcc atcatggtgg
    10501 accgctccag ccgcagcggg cgcccacgcc tgagcgtgtt ggcctggtgc gatccggcac
    10561 aggtgcccga ggccgcctgg cgcggcatgc gctggatgct gtggcaccag ctggcccgct
    10621 cgctgggtgc cagcgcgcgc gtggacacgc agaccgacca tgtgcgtgtc acgctcagcc
    10681 tgagcgccgt caccgaggaa caactggcca ccgggatcga agaatcggtc ggcccgtcca
    10741 gcgtgtcggc ggtgatccag ggctgtcgcg tgctcatcgt ctcgggcaac gcgcaacggc
    10801 gcgcccagag cctgcaggcg ctgaccggct acggcctgat tctcgacagc gcgggcgaca
    10861 tgaccgaggc cgagcgcctg gccacgcggc acctgcccga cgccgtggtc tacgacggca
    10921 gcgtcaaccc gagtgcggtc gcaggtctgc gcgagagcct ggaggcgcgc gctggcacac
    10981 cggccgcctt catcgagatt cacgacgacg acagcgcctc cgatttccgc acctccaccg
    11041 tcggttcggt gtccaccggc cacatcgccg ccggctcgct caatcaggcg ctgggcccgg
    11101 cgctggtgtt cgagctgtgc aaggtgatgt gatccattgc tattgtttta gatagcttgc
    11161 tgcgcagtct ggataagcgc aaaaccgata tttggctcat gttttcgggg tcacccagcg
    11221 gcgcatgagc gcacagccca ggcaccctgc cagcgcagcc gccacaaccg ccgatccgcc
    11281 gctgcaggtg cgctacgaag acgcccacct gatcgcgctc gacaagccgg ccggcctgct
    11341 gaccgtgccc ggtcgcggga ccgacaagag cgactgcctg agccgccgcg cccaggcgcg
    11401 ctggcccgat gcgctcgtcg tgcaccggct cgatcaggcc accagcggcc tgctgctgct
    11461 ggcgcgcggg cccgcgatgc agcgtgcgct cagccaggcc tttgcccagc gccaggtgca
    11521 caagcgctat gaggcgctgg tgcacggccg gctgaccctg ccggccgacg ccaccgacgg
    11581 ctgggcgcgg atcgacctgc cgctgatcgt cgactggccg cagcgcccgc gcagcaaggt
    11641 cgatcacgtg gccggcaagc ccagccagac gcgctggcgc ccgctggcgc atgacgcgca
    11701 gacccggcgc acaagactgg cgctggagcc gctcaccggc cgctcgcatc agttgcgtgt
    11761 gcatctgctg gcgatcggcc acccgatcgt gggcgacacc ctgtacggcc cgcccgacgg
    11821 cgcgccgcgc ctgatgctgc acgcctgcgc gctgcggctg acgcacccgg tcaccggcca
    11881 gccgctcgca ttggacagcg cggcgccgtt ctgacgcacc tgccgacggc ccgcccggcg
    11941 cgctacagtg tgagtcgcgg gcgcgatccc gcagaaaggt tgattgacat gacacgtagg
    12001 aacctgcaag gcctgggcct ggcgctgtcg ctggcgctgg gcggcccgtg gctgtcgggc
    12061 tgcaccatca ccagcacgcc gcaatcgcag gcggcgccgg ccgccacccg cgccgccatc
    12121 gatgcggcgg tgaatgaaac cctgtcgcgt ctgtaccacc tggtgccagg cacgcaggac
    12181 atggtgtcgc gcgccgccgg ggtgctggtg tttccctccg tgctgggcgg ctccttcgtg
    12241 gtcggcgccg aacatggcaa gggggcgctg cgcgtcggtg gccagaccac cagctactac
    12301 agcaccaccg gcgcatcgat cggctttcag gccggcggcc agtcgcgctc ggtgatctac
    12361 gtgttcaaca cgcgcgaggc gctgcagagc ttccgtgcca gcaacggctg gaccgccgac
    12421 gccgacgcca ccgtggcgat cggcaacgtg ggggccaacg gccgcgtcga ttcgcacacc
    12481 gtgcagcagc ccatcgccag cttcgtgctg accaacgtcg ggctggaggc cggggtgtcg
    12541 gtcggcggca gccgcatcgt gccgatcgcc ctgccctgag gagctgcgca cgcgcacccc
    12601 ttcatgcccg ccttctgatc gggcgccgcc caccgccgtc ccatgaaccg caaaccgacc
    12661 cgtcagctgt acgtcctcac cggcgcttcg cgcggcatgg gccgtgccat ggcgcagcag
    12721 ttgctggccc ccggccacca gctgctgacg ctgtcgcgcc ggcccgatga agcgctggcc
    12781 agccaggccg gtgcggccgg cgccacgctg acccagtggg cggtcgacct gggcgacgcc
    12841 gccccggtgg cggcgcggct ggccgactgg ctgcgcgcgc aggacggcaa ggccctgacc
    12901 agcgccaccc tgatcaacaa cgccggcgcc atcccggcca tcgtgccgct gcgcgacgcc
    12961 gacccgccgg cgctggcggc cgccctgcgc ctgggcctgg aagcaccgat gcagctgtgc
    13021 gccgccttcc tgtcggccac gcgcgactgg cccggcacgc gccgggtgct caacatctcc
    13081 tccggcctgg ggcggcgcgc catggcctcg caggccgcct actgcgccgc caaggccggc
    13141 atggaccact tcacgcgctg cctggcgctg gaagaagcgc gcctgcccca gggagcgcgc
    13201 gtgtgttcgc tggcgccggg cgtgatcgac accgacatgc aggtgcagtt gcgcggcgcc
    13261 gatgcggcgg ccttcccgga ccgcgacagc ttcgagcagc tcaagcgcgg cggcgcgctg
    13321 agctcgccgg ccgacgccgc gcgccgtgtg ctggcctacc tggcacggcc cgatttcggc
    13381 cagcacccgg tggccgacgt gcgcgaggtg ctcggagcct aggccaagcg atcgcgcatg
    13441 atctgaatca aactggcctc tcgtccgctt cccgtaagcg ccaccagcta tcgtgtcgat
    13501 agcatctcga agtttcccgg ggctgcggcg cgccggcgcc gcccggccag gcccgcccga
    13561 ccgcccacgc acgccccggt gcggcatact gcctgcctgt cccgagctgt cgttttttca
    13621 ttccaccctt gcaaggagca tccatcatgt cccgtgaagt cgtcgtcgtc agcggtgtgc
    13681 gcaccgccat cggtaccttt ggcggcagcc tgaaagacac ccccaccgtc gatctgggcg
    13741 cgctggtggt caaggaggcg ctggcgcgcg ccaacgtgga cggcaaggac gtgggccatg
    13801 tggtgttcgg ccatgtcgtg aacaccgagc cgcgcgacat gtacctctcg cgcgtggcgg
    13861 ccatcaacgg cggctgcgcc aaggaaacgc cggccatgaa cgtcaaccgc ctttgcggct
    13921 cgggcctgca ggccatcgtc agcgccagcc aggccatctt gctgggcgac tgcgacatcg
    13981 ccattggcgg cggcgccgag aacatgagcc gcgcccccta tgccagcctg gccacccgct
    14041 ttggcgcgcg catgggcgac gccaagatgg tggacatgat ggtcggggcg ctgcacgacc
    14101 ccttccacac catccacatg ggcgtgacgg ccgagaacgt ggccgtcgag cacggcatca
    14161 cgcgcgaggc gcaggacgcg ctggcgctcg agagccaccg ccgcgccgag gtcgcctggg
    14221 ccgagggccg cttcaaggac cagatcgtcc cggtgatgct caagacccgc aagggcgagg
    14281 tggccttcga caaggacgag catttccgcc ccggcgccaa gctggaggac ttccagaaga
    14341 tgaagccggt gttcctcaag gaaaacggca ccgtcacggc cggcaacgcc agcggcatca
    14401 atgacgcggc cgccgcagtg gtgctgatgg aggccggcgt cgccaaggcg cgcggcgcca
    14461 agccgctggc gcgcctggtg ggctacgcgc acgcgggcgt cgagcccaag accatgggca
    14521 taggcccggt gccggccacg cagatgctgc tgaaaaagct cggcctgacc atagaccaga
    14581 tggacgtcat cgaggccaac gaggcctttg ccgcccaggc ctgcgccgtc accaagggcc
    14641 tgggcgccga cccggccaag gtcaacccga atggctcggg catctcgctg ggccacccca
    14701 tcggcgccac gggcgcgctg atcaccgtca aggcgctgta tgagctgcag cgcatccagg
    14761 gccgctacgc gctggtgacc atgtgcatcg gcggcggtca gggcattgcc gcgatcttcg
    14821 agcgcatgtg atcgcacccg cccggttgct gcaagttttg cagctgcctg cgcccgctgg
    14881 acaagcgcca gcgggctttt ttatgggtca aggtggcgcc ctggccgggc gccggagcag
    14941 gcccgcggca tgaggctcga tcccttccgg cgggtggacg acaccccgtt ttccgctcgc
    15001 cgcaccgaca tcgagcgcgc gcgcggccag ccgctgcgcg tcgaacccaa cgccgtcggc
    15061 ctgacggcgc tggactatgg cgatgtggtg ttccgcctgc aggacagcag cggccggctg
    15121 gaggaggtga cgcgccgcgc gccggtgctg tacctgctcc gcggcgacag cgtggtcgat
    15181 gtgccctttg ccgccctggc cggctacgtg cgcacgcacg acgcgcgggc gttcgagcgt
    15241 ggcggctttc tggtcagccc gcgccttggc ctggccttcg tgcccaccga acccgactgg
    15301 gtgaccgcac tggcggcgca ctgcgtcgac gcctggcgcc ggcttggcaa cgatgccgcg
    15361 caggagtgat gagccgccgt caggcgcgca cgaacaatgt gaccagcggc tcgtcgccca
    15421 gctgacggct gcagtggccg tgcacggacg ggtcgaacgc agcgcccggc ggcagcgtgt
    15481 cgttggagta gaagcactgc cccggcccga acacgcgcgt gctgccgtcc tgcagcccga
    15541 tttccatgcg cccctgcagc accaccagcc actgcggcgt ggtggtgcag tggaagtcgc
    15601 tgcgaaaacc caccgggctg tgccggaact ggcagccgcc cgaggcctgc agcgccgaca
    15661 ggcgggccgc gggcgtgcca tcgggcagcg ccagccgatc gtcacgaaag cgcgcccggc
    15721 catcggggcc ggtgtagagc gtggtcaggg tcagcgtggt catggccagg cagtgtagag
    15781 cggcgcgtgc gcacacattt gctcacgctt gcatggcgcc ggcgcaaccc cgtcgggctc
    15841 ggaccgacgt ttcagtgcac tccatcttct caacctggag ttccgcatga aagctcttgc
    15901 caccctgctg ggtgccctcg ccctgagcgg cggcgtgctg ctggccagcc cggcgcaggc
    15961 cgccggccag tggttcgacg tccacgtgat cgaccgcgac agcggcagcc ggctgccgct
    16021 gtaccgccat gacggccagt ggtgggtcgc cgggcgcccc ggcgcgcgct acgccatcga
    16081 actgcgcaac accagcggca cgcgcctgct gggcgtggtg agcgtggacg gcgtcaacgt
    16141 catcagcggc gaaaccgccg actggcagca gagcggctac gtgctggcgc cgcaccagcg
    16201 ggcacagatc accggctggc gcaagagcga ggccgaggtg gcggcgttcc acttcaccgc
    16261 cctgccgcac gcctacgcca cccgcaccgg ccggcccgag cacgtgggag tgatcggcgt
    16321 ggcggtgttt cgcgaacgca cgcccgcacc gcccccgccc gagtggtcgg agcaccgccg
    16381 cgcgccggcg gccggcgaag cgccccccag cccggcggcg ccggcagcgg ccgctgccga
    16441 ttccgaatcc ggcgccgcca gccggcgcga agcccaggcc gccgcaccgc gcctgggcac
    16501 cggccatggc gcacgcgagc cggcctgggt cacccgcacc cggttcgagc gccgcagcag
    16561 ccagcctgac gaggtgatcg ccatccgcta cgacagccgc gaaaacctgc tggccatggg
    16621 cgtgatcccg cagcacccgc gccaggcgca ggcaccgcgg ccgtttcccg acgcgccgcc
    16681 cggcccgggc tacacgcccg acccgccggc ctggcgctga acgctccccg gcgctggcgc
    16741 ctggcgcgcg ccgctggcca taatccagcc catgcccgcg ccagtggcca gcgccgacga
    16801 accgctcatg cgtgcctacg ccgcaggcga cgccagcgcc ttcgagaccc tgtatgaccg
    16861 gcaccagctg cgcctctggc gctacctcct gcgcagcgtg ggcgacgccg ccacggccga
    16921 cgagctggcg caggacgtgt ggctgcgcgt ggcccgcgaa gcgccccgct acacccccag
    16981 cgccgccgcc ccgggccgcc cgccggcgcg cttcaccacc tggctgttca ccctggccca
    17041 ccatcgggtg gtcgatcacc tgcggcgcca gcggccgacc ctcagcctgg acgagccgct
    17101 gcacgacgag ctcacgctgg ccgacacgct ggcagcgccc tcgggtttcg gcccgctgcg
    17161 ccagatcgaa acccgccagc aggccgtgca gctgctggcg gcgctggacg cgctgccgcc
    17221 cgagcagcgc accgccttcc tgctgcaggc cgagggcgaa atgagcgtgg ccgaaatcgc
    17281 cagcaccacc ggcgtcggcc tggaaaccgc caggagccgc ctgcgctacg cccgcgccgc
    17341 gctgcgccgc aagctggagc acctcgcatg aacgcgccgc ccgccctgcc gcgcccgacc
    17401 gacgacgatc tggtcgagcg ctaccacgaa gccgccgcgc tcgaagaccg cggccccgcg
    17461 cccgagctgc gcgcggccgt gctggcccag gcccggatgc tggcctggcg ccatcccgaa
    17521 gcggccgacg acgggccgtc gaacgctacc gaaagcatag ctggtggcga cgatccagca
    17581 agcgctgcag ccgtttttga tgctgaagcc ggcagtcccc gccggccgcg cggcgcggcc
    17641 aacgaccggc gctggctgat ctccaccgct gccagcgtgg cggtgctggg tctggccggc
    17701 ctgctggccc tgcagttcga gcgcagtccg cagggcctgc gcgaagcggc cccgcaggcc
    17761 ggcccgacgg cgctggtgga cgcggcgccg ccgcccgccc cgcccgccac agcggcggct
    17821 ccgctggccc ccacgccggc cgcagcgacg gccgcgcccc cggccgcagc gccgaagccc
    17881 gaaaccatcc agcccgcgcc tgcgcccgca gcgccccatc ctcccgagac cacgccgccg
    17941 gccacgccgc acctgccccc gcccgccgcc gacgccgcca cgccagccac cgccgcgcga
    18001 gcgcccgatc cccgcgcggc caggccgaaa gccgagccgc gcgcccgcga acccgcggct
    18061 cgcccggacc tgcgtcggcc cgccgccgat gcgcgccccg accgaccgcc gcccaccgca
    18121 cccgcgcgcg ccgaacaccc cgaggccgag gccggcgcac ccgccccccg cccgccgcag
    18181 gccagcgagg cgccgtcgcc tgtcccgccc gaccgcgccg ccgcccgcac cggcaccgcc
    18241 agccccaacg gcaccgccag ccccaacggc atcgccagcc ccaacggcac cgccagcccc
    18301 aacggcatcg gcgccgagcc caccgccgcg ccggcggcac cggcggcgcc agcgcccgtc
    18361 ctgaccccgc cgcaacgcct gctggccgcc gccgccgccg gccaggcgga cgccgcccgc
    18421 gccgcgctgg aacagggcgc cgccgtcaac gccaccgaca gcgccggccg caccgcgctg
    18481 atgctgacgg cgcggcgcgg caacgtcggc atcgcgcgca cgctggtgga cgccggggcc
    18541 gacccgcgcc agcccgaccg ccagggcctg accgcgatcg accacgcacg ccgcgccggc
    18601 cacgaggcgc tggcgcgctg gctggaagag cgggcgcgcc cacggtgagc gcgcgcctgc
    18661 ggccacaggc tgctaagctg cgcctgccca tccaccccga cccgcagcga ccaacgcagc
    18721 cgccatgacc gccacctccc atccccccat ctgtttcggc ctgcaaggcc gcgtggtcat
    18781 cgtcaccggc gccgcgcagg gcatcggtga agcctgcgcg cgccgcttcg cccaggaagg
    18841 cgcgcaggtg gtgctggccg acatcgccga cgcacccggc caggcgctgg cgcaggcgct
    18901 gggcgccagc tacgtccatt gcgacgtcgg cgacaaggcg caggtcgacg ccctggtggc
    18961 ccaggtgctg caggcgcacg gccgcatcga cgtgctggtc aacaacgccg gcatcttcaa
    19021 agcggcgcct tttctgcagg tgacggaaga cgacttcgac gccgtgctgc gcgtcaacct
    19081 caagggcgcc ttcctggtcg gccaggcggt ggcgcggccg atggccgagg ccggccgcgg
    19141 cagcatcgtc aacatgagca gcgtcaacag cgtgctggcc atccccgaca tcgccagcta
    19201 caacgtcagc aagggcggca tcaaccagct cacgcgcgcc atggcgctgg cgctggccga
    19261 ccgcggggtg cgcgtcaacg ccgtcggccc cggcaccatc gccaccgagc tggcggcaca
    19321 ggcggtgctg accagcgaag acgcgcgcca ccggatcatg atgcgcaccc ccatgaagcg
    19381 cctgggtgag ccgcgcgaag tcgccgacgt ggtggccttt ctggccagcg acgcggccag
    19441 ctacatcacg ggcgagatcg tcatggtcga cggcgggcgc atggcgctga actacaccgt
    19501 cccggtgtag gcgccatgct gatcgcgcac ctgacggaca cccacctggg catcgactcc
    19561 ggtgcgctgg gcggtcgcct ggacccggcc gccgcgctgc ggcgcgcgct ggcacgggtg
    19621 cgcgcgctgg agcccgcgcc ggccgtgctg ctgctgaccg gcgatctggc cgaagccggc
    19681 cgcgcagccg actacaccct gctcgccgag ctattgaaac aggagctgcc ggcgcatgct
    19741 ggtcaagcgc cgcgggtgct ttgcatcatc ggcaaccacg accaccgggg gcacgcgcgc
    19801 cagaccctgg gcgcgctcat gccgatcgcc cccgaagccc cgccgccgct ggcctgcgtg
    19861 cgcgccgaag tcggtggcct gcagttcatc ggcctggaca cggtgctgcc cggccacccg
    19921 cacggcgcgc tggacgcgcc gcagctcgac tggctggcgc gccagctgcg ggactgcgcc
    19981 ggccagccgg tggcgctgtt catgcaccac ccgccgctga ccagcggcat cgaggccatg
    20041 gaccgctgcg gcctgcggcg cggacgcgcc gagctggccc gcctggtggc cgcgcacggc
    20101 ggcgtgcagt tcatcgcctg cggccacctg caccggccga tcgccggcgt gctgggcggc
    20161 gcgccggtgc tggtggcgcc ctccacctcg caccagctgg agctggacct gcgccccggc
    20221 gcaccgctgg cgctgcggct ggagccgccc atgctgggcc tgtaccgctg gacggcgcag
    20281 gacggcgtga gctgccattt cggccatgtg cagacgttcg aggggccgta tccgatctga
    20341 agtggccggc gcagccgctg cggcgggtcg gctttgggaa ccccggcgca gtgcgcgggc
    20401 cgtcgcacat gctactgaaa gcgcagcttg tggcgcttga caggcaagcg cagcagcccg
    20461 attttcccat cgaccctggc ggccggccgt gccgcctgcc gggcagcggc gcggtggctc
    20521 gcggcgtcgc gcacaatcgg gcgcaccacg acagaccgca aggagacccc atgagcctgg
    20581 cccatctgct gctgcggcag gcgcagcgcc agccgcaggc gcccgccatc ctggacggcg
    20641 cccagccgca cgccagctac ctgcaatggg cggcacgcag cgccggcctg gcccgacacc
    20701 tgcgcgacgg cggcctgcag ccgggcgatc gggtgctgct gttcgcacac aaccacccgc
    20761 gctacctgga gctgctgtgg ggcctgtggt gggccggcct ggtggcggta ccggtgaacg
    20821 ccaagctgca cccgcgcgag gtcgaatgga tcgccgccga cgccggcgcc cagctggcct
    20881 tcgtgacgcg cgacgtggcg cccgacgcgc tggccggcgt ggcgcaccag gtcgatctgg
    20941 agtcgatgga ggccgaccag ctgctggccc cggtggccga gccggcggcg gtgccgatca
    21001 ccgagcgcgc gcccgacgac atcgcctggc tgttctacac cagcggcacc accggccgcc
    21061 ccaagggcgt gatgctgacg cagcgcaacc tgatgaccat gggcctgggc tacttcgcgg
    21121 atgtcgaccc gatcgcccgt ggcgacgcca tcgtctacgc ggcgccgatc tcgcacggcg
    21181 ccggcatcta cgccatcccg catctgatgg ccggggcacg ccacgtggtg ccggcctcgc
    21241 gcggggtgga cgcggccgag ctgttcgcgc tcgggcgcgc ggtggggccg ctgtcgatgt
    21301 tcgccgcgcc caccatcgtc gcccgtctgg tcgaccatgc cgcgcagcag gggctgggac
    21361 cggagctgtg cgcgcgctcg ttcaagacca tcgtctacgg cggcgcaccg atgtacgcgg
    21421 ccgacatcca gcgcgcgctg tcggtgatgg gcccgcgctt cgtgcagatc tacggtcagg
    21481 gtgaaacgcc catggtcggc accgtgctgt cgcgcgccga cctcaaggac cgcgcccacc
    21541 cgcgccacgc cgagcgcatc gccagcgttg gcgtggcgca gacgccggtg cgggtgcgcg
    21601 tgaccgacgc ccaggggcag gacctgccgc tcggcgaggt gggcgaggtg ctgatccagg
    21661 gcgacagcgt gatggccggc tactggggca acccgcaggc gacggcggcg gcgatccgca
    21721 acggctggct gttcaccggc gacatgggcg cgctggacga aggcggctac ctgacgctga
    21781 aggaccgcag caaggacctg atcatcagcg gcggcagcaa catctacccg cgcgaggtcg
    21841 aagaagccct gctcaccgcc gccggcgtgg ccgaagtggc ggtggtgggc cagcccgacc
    21901 ccgaatgggg cgagaacgtg gtggccttcg tggtcgcgca gccgggcgcg gcgccgaccc
    21961 aggaggcgct ggacgcccat tgcctgcagc gcatcgcccg cttcaagcgc cccaagcgct
    22021 acgtcttcgt gcccgagctg ccgaagaaca actacggcaa ggttctgaag accgagctgc
    22081 gccggtggct ggccagcgcc tgagtgcatc agaagaacaa ccggcctggc ggctcagaca
    22141 agccaagaat tgctctatta ttgatagctt ctggcgctca ccagtaaagc gctgtcggcc
    22201 agaatgaaca aaaaagccgc tgcaggcagc ggcttggtgt cagggcgcga ggccgcagcc
    22261 cttacttggc cggcgcgtac ttcttcagca gcgccttggc ttcctcgatc agcttcttgc
    22321 cgtcgtagcc cttggccgcg gcgtccttca accagatctc gtcgacgtcg gcggtggcct
    22381 tttcccagcg cgccagctcg gccggttcga tgacggcgat ggtgttgccg cgatcgcgcg
    22441 ccagcttgga gtacggcccg acgatgttgt cgaagccctc ttcaccggcc caggccgacg
    22501 ccgccaggcc ggagttgtcg tcgatcacct tcttcagctt gggcgacagg ctgtcgtact
    22561 tggcctggtt catgccgaag atgaagatgg tgttggcaaa gcgcggcgag ccgaccgggg
    22621 cgtcgaggtg gtacttgacg atttcgtgca gtttggtggc gggcagcgct tcccagggca
    22681 ccagcacgcc gtcgaccacg cccttggaca gcgcgtccgg cacctgcggc agcggcatct
    22741 gcaccggcgt gccgcccatg gccgacatca tgcgggccga gatgcgggtg gcggtgcgga
    22801 tcttcttgcc cttcatgtcc tccagcttga ggatcggctt gttcgagtgc agcacgtaac
    22861 cttcggtggt gtgcatgaac agcggcttga tgcccttgta ctcgtccagc gcgttcttct
    22921 gcacgtactc ccacagcgcc tgcgagccct gcttggcgtt gatggccatg aagggcagct
    22981 cgaacacttc ggacttggtg tagcggcccg ccgagtaggt gggcaccgac cacacgatgt
    23041 cggccacgcc gtcgcgcacc tggtccagca gttgcggcgg cgtgccgccc agctgcatgg
    23101 cggggtagat ctggcacttg agctcgccac ccgattcctt ttcgatcttg gcgcaccagg
    23161 gcgcgatgat cttgacctgc gagctggagc ccgaaggcag gaagtggtgc accttcagca
    23221 cggtctgcgc ctgggcggcg gtgcccgcca gcagcgacag cgccgcggcc gccatcagtt
    23281 tgttcagctt tgagctcatc aaggtatctc ctggttgaaa agggttgtct ttcaggtcag
    23341 caggcgcacc agccacaggg tgatgccggg gaacatgatc agcaggccca gacggatgaa
    23401 gtcccaggcc aggaagatga acacgccctt gtaggtctcc accatcggca catccttggc
    23461 caggccgttc atcacataaa cgttcagacc gaccggcggg aagatcatgc cgatggcgca
    23521 aacggtaaga atgagtatcc cgaaccacag cgccttgtcc gacgcgtcga gaccgaagaa
    23581 gtccatgccc atgatgaccg ggaacaccac cggcaccgtc agcaggatca tcgacatttc
    23641 gtccatcacg cagcccagcg ccacgtagaa cagcatgatc acggccatca ccagcaccgg
    23701 tggaatggcg gcgtgggcga tcagctcggc cagctggttg ggcagctgcg acagcgccag
    23761 cgaggcgttc atcatgtcgg cacccaggaa gatcatgaag atcatgcccg aggtgacggc
    23821 cgtggtgcgg atcgactcga tggccttctt cagcgtcagc tggcggttga tcagcgtgat
    23881 ggcgaaggtc agcgtggcgc cgatggccgc gccctcggtg ggcgtgaaga tgccgccata
    23941 gatgccgccg aacacggcga cgaagatcag cgcgatcggc cacacgccgg cggccgactg
    24001 cgccacctcg gacgcgctgg cggcgcgcgc ctcgggcgcg tgggtggggt gcaggcgcac
    24061 gtagatggcg atcaccacca ggtaacccag cgccgccagc atggccggca cgtaggcggc
    24121 caggaaggtc ttggtgatgt tctgttcggt caggatggca tacaccatca gcgtcaccga
    24181 aggcggcagc agcacgccca tggtgccgcc ggtggccagc gccgccgtcg ccaggcgccc
    24241 cgaatagctg atgcgacgca tttcgggata ggccacctgg gcgatggtgg cggtggtcgc
    24301 cacggtggag ccggaaatgg cgccgaaggc cgcgcaggcc aggatgccgg ccatcgccat
    24361 gccgcccttg aagcgcccca gcagcgcgtt ggcaaagtcg aacagcgcct tggacaagcc
    24421 gccgtgcacg gcaaaaccgc ccatcagcat gaacagcggg atcaccgaca ggtcgtacac
    24481 cgacacccgg ccgtacaccg ccccgcgcag gtgtgccagc agcgggtccc agcccgacag
    24541 gatcacgtaa cccaccgcgc cgggcacgaa catggcgacg gcaatcggca cccggatcgc
    24601 catcagcagc agcatggcga cgaacagcag caaaccgatg gtcagggtgc tcatgcggcg
    24661 tctccctcgg ccgcggcggg ctgcgtccag tagatcaccg cctggtacag gccgaccagg
    24721 gtgcacagca ccaagccggg caccatcagc atgtagggaa tccagagcgg caggttcatc
    24781 agcatcgagg cttcctggtt ctcctggctc atcacgccac cggcatacac ccgccaggcg
    24841 atcagcgccg tcatgccggc atacagcagg cagcccaggc cgtccatcca gcgctggctg
    24901 cgcgccgagg ccctggtggt gaagaagtcg acaatgatgt tggcgctgcg aaactgcgtg
    24961 tacggcagga aggtggccac gcacaccgcc aggccgagct gaaccagttc cacatcaccc
    25021 aggatcgggc tgtcgaaaaa ggtgcggccg atcacgctca ccaccgtcat gatggccatg
    25081 gcgatcagca ccgccccgcc gaccacggcg cagatgtcgc acagcacgtg cagcaggcgc
    25141 cccaccggcc cctcgcgcat tcgattgtct gccgtcatcc cggctcatct cctcttgagt
    25201 atccgtggcc gtacccgaca gccgcaagcg cacgccgcca caggacagcg ttgacgggcg
    25261 gcaagtctaa ccagtctcca cccgtccaca cacaggccaa tccctacgcg gggccgctta
    25321 ttctatggca aatagattcg ctcatgttta acctggaaac ggcagttggc cgcttgtcat
    25381 cttcgggcag accgcatcga tgcggggtct ggcggctgcg atggcctgcg cctcctgggt
    25441 gagcacgctg cgcacccacc cgcaccgcgc tcggcgcgcc atgcggcgtt gctcctcctt
    25501 gcgcgcagga gcccgcggcg tcgtcgcgtc ttgcatggca caccgatcac ggcccgggcg
    25561 ggcgcgtcca acggccgtgt ctaggtttca tcgtgcgggt tgtcgtgtat tgaaggtgca
    25621 agctcggttc gacacatccc gtggcggtgg cgggcgcccg gccagcggcg atccg
//
LOCUS       DAOQTZ010000031        25623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2250_length_25623_cov_13.767678, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000031 DAOQTZ010000000
VERSION     DAOQTZ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 25623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25623
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2250_length_25623_cov_13.767678"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PKN51_05470"
     CDS             complement(<1..945)
                     /locus_tag="PKN51_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT84583.1"
                     /translation="MSLAQSLPYEMLLGWRYTRAGRGTRRNGFISFISAASMLGIALG
                     VAALIVVLSVMNGFRKDVTERMLSVISHVEVFSPDGEALPDPARTLAEVRANPEVVGA
                     APFIGAQALLARGESMQGTIVRGIDPAREPFVTEVAAQLQATLLPRLVPGSYGILLGS
                     DLARKLGVAEGDAVTLIAPSGQVTPAGVVPRLKQMTVVGTFSSGHYEYDSALAMLHID
                     DAARIFRLQGPTGIRLKLRDLHRARSVADQLAASLSGHLLILDWTRQNRTWFAAVQLE
                     KRMMFIILTLIVAVAAFNLVSTLVMTVQDKRADIAILRT"
     gene            1012..1962
                     /locus_tag="PKN51_05475"
     CDS             1012..1962
                     /locus_tag="PKN51_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HNT84584.1"
                     /translation="MSAAPAWPHVLIPDAVLALPDGAAAPPWPELPALRALARQLRLA
                     ERLTLDEDSPATPFEVALARAHGLPDTPGRTPWAAFESGTSGTPCAWLLPCHWQMGMN
                     DVSVLHPAELGLDERESRALLASVEPLLRDDGLTLRYLRPDAWLVQGELLRGLSCWSL
                     RRALTRPVTRQQLAQAPTEAQRRTLRRLQSEWQMLLYTHPVNEAREGARRWPVNALWI
                     GGAGELPQAIAPRADVQVCRELLDLPARLSLHDWQRAWQAIDANALAPLLQTVRGGAP
                     ARLTLCGPRRALTLSSAQGLTQKIMSQISPLRLSNLRDQL"
     gene            1959..3668
                     /gene="recJ"
                     /locus_tag="PKN51_05480"
     CDS             1959..3668
                     /gene="recJ"
                     /locus_tag="PKN51_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNT84585.1"
                     /translation="MKIEVRDVPPRAAWALEQAGVHPLLARLYAARGVSSAAELDDGL
                     AQLLPPDRLQGAQAAAVLLADAIAAGRRICIVADYDCDGATACAVGLRGLRLLGATQL
                     DYLVPDRVIDGYGLTPPIAERVAARGAELLITVDNGIASVEGVARARALGLQVLVTDH
                     HLPGGALPPADGIVNPNQPGCGFESKALAGVGVMFYVLLALRAELRARGRFSAATQPR
                     LDSLLPLVALGTVADVVPLDANNRRLVAQGLRRIRAGQMPVGLAALFAVAGRATPRAG
                     SFDFGFALGPRINAAGRLADMTLGIELLLTDDAARAAELAATLDAINRERREIEGGMR
                     EQALQIAEGLFEPHADPPAALAVFDPDFHEGVVGIVAARLKDRLHRPTFVFAASGAPG
                     REHELKGSGRSIAGFHLRDALDLVAKRHPGVLLRFGGHAMAAGCTIDEHQFDTFERAL
                     QQVATEWLDAATLTRRLHTDGALPPEYRRPDVVDLLHDAVWGQGFAPPVFSEELEIVG
                     QRLVGEKHLALKLRHQGQPVDGIWFGRTEPLPARALLAFRLEADEWQGVRRVRLLIEG
                     LGA"
     gene            3827..4600
                     /locus_tag="PKN51_05485"
     CDS             3827..4600
                     /locus_tag="PKN51_05485"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HNT84586.1"
                     /translation="MAAFDDEKVLSVHHWTDRLFSFTTTRSQSLRFSNGHFTMIGLRV
                     NGKPLLRAYSIVSPNYEETLEFLSIKVPDGPLTSRLQHIQPGDSIIVGHKPTGTLLID
                     YLLPGKRLYLFGTGTGLAPFMSIIRDPDTYEKFDQVILVHGCRLVSELAYRDYITQEL
                     PRHEFLGELVSSQLRYYPTVTREPFPTQGRIPELIESGRMARDLGLPDLHPQLDRVML
                     CGSPEMLKSIKEVLEKRGFKEGNTTTPGDFVVERAFVEQ"
     gene            complement(4634..4960)
                     /locus_tag="PKN51_05490"
     CDS             complement(4634..4960)
                     /locus_tag="PKN51_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNT84587.1"
                     /translation="MGSELRFAATVANYGSCAFKQYQLVLSDTDRPLLMLMGGRDAEV
                     PASACFPLLEQMKAAGKPVEWHLYPQATHAWDKPGLPARGYVYDAQVTQDATARMVAF
                     FAAGGR"
     gene            complement(4973..5479)
                     /locus_tag="PKN51_05495"
     CDS             complement(4973..5479)
                     /locus_tag="PKN51_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84588.1"
                     /translation="MHLRKPLDRLLLATALLIVHWGVGAQDLQAQMAQPLEFKPVAFS
                     DEIKDSIGLFTNVANTVFKPAGDGPFPATVLMHTCGGLKGPPNAHMKQHAQSLLAAGH
                     VVLVIDSFGPRGFDSCATRVLSHSAGIADAYAALALLAGKPFVDKSRIYQAGYSWGAF
                     VSTHLASP"
     gene            complement(5556..6056)
                     /gene="coaD"
                     /locus_tag="PKN51_05500"
     CDS             complement(5556..6056)
                     /gene="coaD"
                     /locus_tag="PKN51_05500"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNT84589.1"
                     /translation="MSQPVIAVYPGTFDPITLGHEDIVRRAARLFERVVIAVATAHHK
                     KTLFTLDERLAITREALADLPQVSVVPFTGLVTAFAVAQGARVMVRGVRSVTDFDYEA
                     QLAGMNRALVPEVDTIYLTPSSATQHISSTLVREISMLGGDVAQFVSPPVLAHLMRRH
                     GERGRP"
     gene            complement(6110..6718)
                     /gene="rsmD"
                     /locus_tag="PKN51_05505"
     CDS             complement(6110..6718)
                     /gene="rsmD"
                     /locus_tag="PKN51_05505"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNT84590.1"
                     /translation="MSQPSKPAARGRAALPRQVRVIGGQWRRHLLPVPDRPGLRPTPD
                     RVRETLFNWLGQTLTGWQVVDAFAGTGALGLEAASRGAAEVLLVERDDGLAAGLRALK
                     QRLGAAMVRVQAGDGVAALAALAPASVDLVLLDPPFDQPALLAPALTAAARAVRPGGF
                     VYLETPQRWDDAALDALGLALHRHLKAGAVHAHLLQRPAPSA"
     gene            6906..7451
                     /locus_tag="PKN51_05510"
     CDS             6906..7451
                     /locus_tag="PKN51_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNT84591.1"
                     /translation="MSAKPQNPVDTDAPLTNFSHCHAGILRHLDDFGQLPALLEPAQR
                     LRQIAERFVEFFREAVFEHHQEEERELFPAVLASAAPGAERERVQQMVDQLTREHRDV
                     EAQWKRLEPQIKKLAKGQPADVDPQDVRSLVAQYQAHARFEEQEFLPLSEQILSRNSD
                     HMAALGLSLHMRHQPAFLSHI"
     gene            complement(7642..8463)
                     /locus_tag="PKN51_05515"
     CDS             complement(7642..8463)
                     /locus_tag="PKN51_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNT84592.1"
                     /translation="MTTPIPRTPPPEGCIDTQVIDDQILLIGLNRPAKRNGWTPRMFR
                     ELAEAYTRLDDDPTLRVGVLHAFGEHFTAGLDLPLVVEHLQRGEPLTPYGLVEPHELG
                     LPGYRRRQKPMIAAVQGICFTVGIELMLAADIAIAADNCRFSQMEVQRAILPAGGATL
                     RMAERAGVGNAMLHLLTADQFDSAEALRCHFVQKVVPAAELLDSALTLARRIAAQAPQ
                     AVVATRLNVLKAIELGQAAAVADFGPVQRRLAASEDAAEGVRAFIEKRPARFTGR"
     gene            complement(8510..9874)
                     /locus_tag="PKN51_05520"
     CDS             complement(8510..9874)
                     /locus_tag="PKN51_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT84593.1"
                     /translation="MKTIAVRALLVCSGAFFHSVSAWAAIPIQHWTQPSGAQVWLVES
                     PAIPMLDVQLDFDAGSRRDPPAQAGLASATALLAGKGVAARGEQPALDENQLGEAWAD
                     LGASFGASAGADRMSFALRTLTDAELLRQAVQLAARELGEPAWPEPVWQRERARLSAA
                     IQEARTRPATQAALAFQQAVYGRHPYGQDSTPETLARIQVADLQAFYRQHVQPCRARV
                     SLVGAVDRAQADALVRELLGRLPSAGACAPLPPVAEVAPLQAAREIRIPFQSAQAHVL
                     IGQPGYKRSDPDFFALLVGNHILGGGGFTSRLMREVREQRGLSYSVYSDFAPGLHAGA
                     FSIGLQTRPDQADAAVAVVRQVLADFVAQGPSADELKAARDNLIGGFALRLDGNRKLL
                     ANVANIAWNGLPLDYLEHWSARVAAVTLADIRAALQRKLQPERMVTVVVGAPEAGQRN
                     GNDR"
     gene            complement(9948..11375)
                     /locus_tag="PKN51_05525"
     CDS             complement(9948..11375)
                     /locus_tag="PKN51_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT84594.1"
                     /translation="MNFRPAFLLPVLLAALLGACASAPPPAATDAASPAAAAGAVQRF
                     TLDNGLTVIVKPDRRAPTAVHMLWLRVGAMDEVDGTSGVAHVLEHMMFKGSERLAPGE
                     FSRRVAQLGGRENAFTSLDYTGYYQQVPAARLGEVMALEAERFAHLRYSDEVFAKELE
                     VVKEERRLRTEDNPRALLFEQQRAVGFIASPYRRPVVGWMGDLQAMTADDARAFHRQW
                     YVPANAAVVVVGDVEPAAVLALAQRHYGGMAARPLPGRKPRPEPAQLGLRRIEVKAPA
                     EQAVVSLAFRVPRLDGLEPGADNDDALALTVLAAVLDGYSGARLERALTQGPDRVADS
                     ASAGNGLLGRGPQWFTLGGVPATGKSAAQVEAALRAEVARVAEQGVSPAELARVKTQW
                     VASEVYKRDSVMGQAQELGHLWVQGLPLDADQRLVARLRQVTAAQVQAVARKYFGDDQ
                     LTVATLVPQPRAAGATRPAPAPTRH"
     gene            11482..12558
                     /gene="selD"
                     /locus_tag="PKN51_05530"
     CDS             11482..12558
                     /gene="selD"
                     /locus_tag="PKN51_05530"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNT84595.1"
                     /translation="MNSPAAAPATPTPEPRLTSLSHGGGCGCKIAPGVLSAILKDTLA
                     MPMPPELLVGIQTADDAAVYRLNDEQALVATTDFFMPIVDDPFDFGRIAAANALSDVY
                     AMGGTPIMALALVGMPINVLSLQTIGRILEGGASVCRDAGIPVAGGHTIDSVEPIYGL
                     VALGLVHPERVKTNSGAQVGDRLVLGKPIGVGVQSAALKKGALPAAGYAAMLASTTQL
                     NRAGPALARLPGVHAITDVTGFALAGHALELARGAGVTVHIDWPSVPLLPGVGQLAAQ
                     GMVTGASGRNWAAYGHDVALPAGFGDSQRALLTDPQTSGGLLVACDAASVDEVLATFR
                     HSGFAQAAVIGRIEPAGASPLVVA"
     gene            12805..13944
                     /locus_tag="PKN51_05535"
     CDS             12805..13944
                     /locus_tag="PKN51_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84596.1"
                     /translation="MTQQLPRPHATPAVAGGIRLGQATATVNEALQLRRYLLALGLTE
                     HVTRQDGIWRLDPEAAQRWLPAGDTLGLHQRLAARWQPDALELRRQIVVAMLAGPWVF
                     EFPSCAELRAAVRVRERIVRAARQTALAFRTEAAERPAEDWCYHPDTGFTLRPGRGLI
                     AALRKATQPRVSGRLYDFSCYRATEYVILLALAQEAARSNPALLARLQRQWECKAIMS
                     GSFHDTFLREYGHQDTPLPTRYYVPGDRLWFRNPDEASADATGYEGSWVFYLGHGEFS
                     DFWRAGRTYTLESKCLEIYHWRDGLYRDARTGASQIDEGRVAQQVAATLRDPAGHARI
                     QASMQRYREPRGCYTDAGGCIDTTREYPRCICPADCEIDLPDAPI"
     gene            13979..15157
                     /gene="ftsY"
                     /locus_tag="PKN51_05540"
     CDS             13979..15157
                     /gene="ftsY"
                     /locus_tag="PKN51_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNT84597.1"
                     /translation="MFSFFRRKKAAEPAPENAVPEAAPADPAAELAEAATPLRADELE
                     AQALAAAPEEGPHPAPSVAIETEAAGAAPTSAEVEFDHESANLAQPTPAARSGWLDRL
                     KSGLKKTGSSITTVFTGTRIDEALYEDLETALLMADAGVPATETLLADLKRRVKEARA
                     TEPAQVRALLQDAIADLLAPLEKPLVVGQYHPTVIMVAGVNGAGKTTSIGKLTRHLAE
                     AGATVLLAAADTFRAAAREQLAIWADRNAVEIISQQGADPSAVAFDAVAAGRARGRDV
                     VLVDTAGRLPTQKHLMEELAKIRRVVTKADVTAPHEVLLVIDGNTGQNALAQVRAFDE
                     AAQLSGLIVTKLDGTAKGGVLCAIAREKPVPVYFIGVGERLEDLQTFRAREFAQALVG
                     "
     gene            15261..16352
                     /locus_tag="PKN51_05545"
     CDS             15261..16352
                     /locus_tag="PKN51_05545"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT84598.1"
                     /translation="MSVNPPRIHLVLLGAGRAHMQVLGSLARQGSGHLAVTLVAPHPY
                     YIERAMLAGYVAGDYALEQLRLPLDRLIERSGASFVPAHVIALDPDGRRVQLSSGDAL
                     PYDVLSINLEPALERERIEQAMPGARANALFTHPLETFVHLWPQLLALAGERALQVAV
                     VGDGLPAVELALAVAHAVAAPHGSRVTLVAGSAPLLTDPSSALQRRLLARLKQSHITL
                     LHDQCTGIASGVMQLASGATLACDAPLLATGGGTPGWLMQAGLLANDAGEPLVNERLQ
                     SDSHRQIFIVPDDAPAEVGPVLDANLRTAVGGGSFRKAPGEPPRLRIVSCGSGQAVAA
                     WGPLGLEGREVWHWKDRRDRRQLAALFAV"
     gene            complement(16657..17433)
                     /locus_tag="PKN51_05550"
     CDS             complement(16657..17433)
                     /locus_tag="PKN51_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNT84599.1"
                     /translation="MHLAPADTIAGLVKGLAVLESFDTERQRLNATQAAQRAGLTRAA
                     ARRHLLTLAHVGYLESDGQFFWLSPRALRLAGAYLASARLPRVVQPALNRLAAATHDV
                     YSAVVLDGDEVVIIARSAGLGAPGTRLLAYGMHLGARLPAHTTSTGRVLLAALPRPAL
                     TDWLAHHQALPRLTPHTTTGTRAFKALLAQVARQDWALSSEENELGVHALAVPLRNAQ
                     GATVAALNVIVPPARFSDALMQQELLPRLREAARELRQML"
     gene            17555..18751
                     /gene="pobA"
                     /locus_tag="PKN51_05555"
     CDS             17555..18751
                     /gene="pobA"
                     /locus_tag="PKN51_05555"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HNT84600.1"
                     /translation="MTTTRTQVAIVGAGPSGLLLGQLLHKAGIDAVIVERVSGDYVLG
                     RIRAGILEQVCIDLMDEAGVGARMHREGLVHGGIEMLWDGRRHRVDLNRYSGGKNVMV
                     YGQTELTRDLMDARAAAGLTTVYEAHHTAVHDFDTARPYVTYVKNGVTHRIDCDFIAG
                     CDGFHGVCRASAPRSAIREYEKVYPFGWLGLLSDTPPVCDELIYVNSERGFALCSQRS
                     KTRSRYYLQVPLTHHVEDWSDDAFWQELRLRLDDEARERLVTGPSLEKSIAPLRSFVT
                     EPLRFGRMFLAGDAGHIVPPTGAKGLNLAATDVKYLFDGLVQHYRERSDAGLDAYSAK
                     ALARIWRAERFSWWFTQLMHRFPHDEQITAKFQHAELDYLMHSEAGLRTIAENYVGLP
                     LDFGQR"
     gene            18916..19827
                     /locus_tag="PKN51_05560"
     CDS             18916..19827
                     /locus_tag="PKN51_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNT84601.1"
                     /translation="MPVPAPIRPLRGTRIVSLALNLPGPAALMRLHALGARCLKVEPP
                     GPGRTAAGRPIPGDPMGQYDVGAYGVLHQGIRTVSMDLKSERGRARLATELAGAELLL
                     TSFRPSALAKLGLGWRALHRAYPTLSLVEIVGAPGERAEEPGHDLTYLAEAGLVTGTE
                     LPPTLLADMAGALLAVEAALAALMQARASGRGVHRQVALSDAAHWLALPRNWGLTLPS
                     GAVGGAHAGYRVYPCKDGRVAIAALEPHFASALFKVAGLPAPDLRAPLAPQARDAIAA
                     WALGKTRQQLDALARQHDIPLHTLPRA"
     gene            19833..20534
                     /locus_tag="PKN51_05565"
     CDS             19833..20534
                     /locus_tag="PKN51_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HNT84602.1"
                     /translation="MRGEGSIRRWDAARGFGFIQTAERRDVFFHVRDFRGAGAPTEGL
                     RVSFEAIEVGGKGPRAMDVRPLSATAGPAATTPAARPQPTAPRSASATAARARPEQVP
                     SRAHAGPPPAPHGAALAWLLMLGWLGLLAAGAWWQRLPTWLLPVLVVLNLATYLAYAG
                     DKRAAQRGHWRTPEQTLHLLALLGGWPAAWWAQQWLRHKSRKLEFRLAYWVTVVLNCA
                     ALALLVARPTWLPAP"
     gene            20642..22054
                     /locus_tag="PKN51_05570"
     CDS             20642..22054
                     /locus_tag="PKN51_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNT84603.1"
                     /translation="MRERGLAPEFGAAVRQQLDTIDGPAEDEGPDIDDLTGLLWCSID
                     NDDSRDLDQLTVSETLPDGGVKLLVAIADVDALVSKDTPIDRHAQINTTSIYTSARIF
                     PMLPEKLSTDLTSLNPHQVRVATVTEMVFAPDGTLLRSHIRRARVRNQAQLAYDAVSA
                     WLEGQGPLPEAADRVPGMDDQLRTQDALAQQLRANRREQGALEFQTLQPRAEFEGERV
                     VAIRQQEQNRARQLIEEFMVATNGVTARFLAGKRRAAIRRVVRSPERWARIAEVAAER
                     GWRLPPDPDAHALEQFLAAERRRDPLRFPDLSLVIVKLMGRGEYVVERPGGPVIGHFG
                     LAVRDYSHSTAPNRRYPDLITGRLLKAALAGERPPYSDTELEFLANRCTSQEDAANKV
                     ERRLRKSEAAMLLEGQIGKVFDGVVTGQSKGSTWLRIFDPPADGKLLASRELKVGDKV
                     RARLVFTHVEHGFIDFEQID"
     gene            22116..22346
                     /locus_tag="PKN51_05575"
     CDS             22116..22346
                     /locus_tag="PKN51_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84604.1"
                     /translation="MGSVISFYIDQDSPGFRTTGLLPSPEDDIAPCALSVGDTVSFPN
                     HRNRRYRVVSRHCNAGADMDEPQWLLRLEPLR"
     gene            complement(22540..23142)
                     /locus_tag="PKN51_05580"
     CDS             complement(22540..23142)
                     /locus_tag="PKN51_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HNT84605.1"
                     /translation="MTRSASDVLRFWLGAHPLDSAAMQRVQAQWFRKDETFDARLREG
                     FGATIAAARAGRLDRWADTAEGRLALLIVLDQFTRNAFRGQPDGFAGDAQALALALRG
                     IELGHDQAVPPMARIFCYLPLEHAEDATLQARNVALFQALRDAPGAEPRAFFDGTLDY
                     ALRHQDVIARFGRFPHRNAILGRTSTAAELDYLAQPGAGF"
     gene            23262..25310
                     /gene="fusA"
                     /locus_tag="PKN51_05585"
     CDS             23262..25310
                     /gene="fusA"
                     /locus_tag="PKN51_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT84606.1"
                     /translation="MPIAAPTVAQIRNLALVGPAASGKTTLAEALLHRAGAIGAMGSV
                     ERGSTVSDHDPQEIKAQHSLQSSLLHLDHDGCRVHLMDTPGLPDFIGQSLPALEAVET
                     CAVVINAATGIEPMARRMMDWAAERQQDRLIIVNKIDAEGVDLPALLADIQAQFGSEC
                     LPLNLPDAGATKVVDCFYNRDGHSDFGAVADAHRALVEQVVEVDGDFVERYLNDGDID
                     AAELHAPLEQALREGHLIPVCFVSARSGAGVAELLDIITRLLPNPIEGNPPDFFNGEG
                     EAATLMHARPDPAAHVLAQVFKISIDPYVGRLAYVRVHQGTITPASQLFIGDGRKPFK
                     VGHLFLQQGKNHVEVPSAGPGDICTIGKIDELHYDAVLHDAAEDAHLHLRPLPFPVPV
                     HGVAIRPKKRGDEQRLWEIMAKLVAEDPCLKIERLASTHETVVYGLGELHLRLLLERL
                     RDGYKFEVDTQPPRIAYRETIAQNAAAQYRHKKQSGGAGQFGEVHLRVEPLARGAGFE
                     FVDQVKGGTIPGQFMPAVEKGVREALAEGVIAGYPVQDLRVIVHDGKHHPVDSKEVAF
                     VTAGKKAVQAAIREARPIVLEPIARLAITIPDSATGAVTGDLSSRRGIVSGTDSPRPG
                     QVSVTAQVPMAELASYQMRLDALTAGQGAYTLALSHYEAVPPQVQQQLAGQYKVKEEE
                     "
BASE COUNT         3818 a         9468 c         8854 g         3483 t
ORIGIN      
        1 ggtgcgcagg atggcgatgt cggcgcgctt gtcctgcacc gtcatcacca gcgtgctgac
       61 caggttgaag gcggccacgg cgacgatgag cgtgaggatg atgaacatca tgcgcttttc
      121 cagttgcacg gcggcgaacc aggtgcggtt ctggcgcgtc cagtcgagga tcagcaggtg
      181 gccgctcagg gacgcggcca gctggtcggc cacgctgcgc gcccggtgca gatcgcgcag
      241 cttcaggcgg atgccggtcg gcccctgcag gcggaagatg cgtgccgcgt cgtcgatgtg
      301 cagcatggcc agcgccgagt cgtattcgta gtggccgctg ctgaaggtgc cgaccaccgt
      361 catctgcttg aggcgcggca ccacgccggc gggggtgacc tggccgctgg gtgcgatcag
      421 cgtcaccgca tcgccctcgg ccacgcccag cttgcgcgcc aggtcgctgc ccagcaggat
      481 gccgtagctg cccggcacca ggcgcggcag cagcgtggcc tgcagctgcg ccgccacctc
      541 ggtgacgaag ggctcgcgcg ccgggtcgat gccacgcacg atggtgccct gcatggactc
      601 gccgcgcgcc agcagcgcct gcgcgccgat gaagggcgcg gcgcccacca cctcggggtt
      661 ggcgcgcacc tcggccaggg tgcgcgccgg gtcgggcagc gcctcgccgt cgggcgaaaa
      721 cacctcgacg tgcgaaatca cgctgagcat gcgctcggtg acgtccttgc gaaagccgtt
      781 catcaccgac agcaccacga tcagcgccgc cacgcccagc gcaatgccca gcatcgacgc
      841 cgccgagatg aacgagatga agccgttgcg ccgcgtgccg cgcccggcgc gcgtgtagcg
      901 ccagccaagc agcatttcgt agggcaggct ctgggccagg gacatcgggt ggaaggcggg
      961 gcacgccgtg caggacaagc ccggcattgt ggcatcccgg acaatgcggc catgtccgct
     1021 gcacctgcct ggccgcatgt cctgatcccc gatgccgtgc tggccctgcc cgacggcgcc
     1081 gcggcgccgc cctggcccga gctgccggcg ctgcgcgccc tggcgcgcca gctgcgcctg
     1141 gccgagcgcc tgacgctgga cgaagacagc ccggccaccc cgttcgaggt ggcgctggcg
     1201 cgcgcccacg gcctgcccga cacccccgga cgcacgccct gggccgcctt cgaaagcggc
     1261 accagcggca ccccctgcgc ctggctgctg ccctgccact ggcagatggg catgaacgac
     1321 gtcagcgtgc tgcacccggc cgagctgggg ctggacgagc gcgaatcgcg cgccctgctg
     1381 gccagcgtcg agccgctgct gcgcgacgac ggcctgacgc tgcgctacct gcgccccgac
     1441 gcctggctgg tccagggcga gctgctgcgc ggcctgagct gctggtcgct gcggcgggcg
     1501 ctgacccggc cggtcacgcg ccagcaactg gcgcaggcgc ccaccgaagc gcagcgccgc
     1561 acgctgcgcc gcctgcaaag cgaatggcag atgctgctct acacccaccc cgtcaacgaa
     1621 gcgcgcgaag gcgcgcggcg ctggccggtc aacgcactgt ggatcggcgg cgccggcgag
     1681 ctgccccagg ccatcgcccc gcgggccgac gtgcaggtgt gccgcgaact gctcgatctg
     1741 ccggcccgcc tgagcctgca cgactggcag cgcgcctggc aggcgatcga cgcgaacgcg
     1801 ctggccccgc tgctgcagac ggtgcgcggc ggcgccccgg cgcggctcac gctgtgcggc
     1861 ccgcgccgcg cgctgacgct gtcgagcgcc cagggcctga cccagaagat catgagccaa
     1921 atcagcccct tgcgcttgtc caatctgcgc gaccagctat gaaaattgaa gtccgcgacg
     1981 tccccccgcg cgccgcctgg gcgctcgagc aggccggcgt acacccgctg ctggcgcgcc
     2041 tgtacgcagc gcgcggcgtc agttcggccg ccgagctcga cgacggcctg gcgcagttgc
     2101 tgccgcccga ccggttgcag ggcgcgcagg ccgccgccgt gctgctggcc gacgccatcg
     2161 ccgccgggcg gcgcatctgc atcgtcgccg actacgactg cgacggcgcc accgcctgcg
     2221 ccgtcggcct gcgcggcctg cgcctgctgg gcgccacgca gctggactat ctggtgcccg
     2281 accgcgtgat cgacggctac ggcctgacgc cgccgatcgc cgagcgcgtg gccgcgcgcg
     2341 gcgccgagct gctgatcacc gtggacaacg gcatcgccag cgtcgaaggc gtggcgcgcg
     2401 ccagggcgct gggcctgcag gtgctggtga ccgaccacca cctgccgggc ggcgcgctgc
     2461 cgccggccga cggcatcgtc aatcccaacc aaccgggctg cggcttcgag agcaaggcgc
     2521 tggccggcgt gggcgtgatg ttctacgtgc tgctggcgct gcgcgccgag ctgcgcgcgc
     2581 gcggccggtt cagcgccgcc acccagccgc ggctggacag cctgctgccg ctggtggcgc
     2641 tgggcacggt ggccgacgtg gtgccgctgg acgccaacaa ccgccgcctg gtggcgcagg
     2701 gcctgcggcg catccgcgcc ggccagatgc cggtcggcct ggcggccctg tttgcggtcg
     2761 cgggccgggc cacccccagg gccggcagct tcgacttcgg ctttgccctc ggcccgcgca
     2821 tcaacgccgc cggccggctg gccgacatga cgctgggcat cgagctgctg ctgaccgacg
     2881 acgccgcgcg cgccgccgag ctggcggcca cgctggacgc catcaaccgc gagcggcgcg
     2941 agatcgaagg cggcatgcgc gaacaggcgc tgcagatcgc cgaagggctg ttcgagccgc
     3001 acgccgaccc ccctgccgcc ctggccgtgt tcgaccccga cttccacgaa ggcgtggtcg
     3061 gcatcgtcgc cgcccgcctg aaggaccggc tgcaccgccc gaccttcgtg ttcgccgcca
     3121 gcggcgcgcc cggacgcgag cacgagctga agggctcggg ccgctcgatc gccggctttc
     3181 acctgcgcga cgcgctggac ctggtggcca agcgccatcc cggcgtgctg ctgcgctttg
     3241 gcggccacgc catggcggcc ggctgcacca ttgacgaaca ccagttcgac accttcgagc
     3301 gcgccctgca gcaggtcgcc accgagtggc tggacgccgc cacgctgacg cgccgactgc
     3361 acaccgatgg tgcgctgccg cccgaatatc gccgccccga cgtggtcgat ctgctgcacg
     3421 acgcggtctg gggccagggt ttcgctccgc cggtgttcag cgaggagctg gagatcgtcg
     3481 gccagcgcct ggtgggcgaa aaacacctgg cgctcaagct gcgtcaccag gggcagccgg
     3541 tcgatggcat ctggttcggc cgcaccgagc cgctgcccgc gcgcgccctg ctggcgttcc
     3601 gactggaggc cgacgaatgg cagggcgtgc gccgcgtacg cctgctgatc gaagggctgg
     3661 gcgcctgatc ccgcgagcga ccgtcggaga aggtgcgagc cttccgacgc gcctgccatc
     3721 cgggcgcgcg ccgcgcgcat gacggtatgc cggcaggtca tgaaggctgg gcgacaatca
     3781 gggttttcca cccggtgcgc cgcgcgcacg ttcaccgcaa gacgtcatgg cagccttcga
     3841 cgacgaaaaa gtcctctcgg tccaccactg gaccgatcgc ctgttttctt tcaccaccac
     3901 gcgcagccag agcctgcgct tttccaacgg ccacttcacc atgatcggcc tgcgggtgaa
     3961 tggcaagccg ctgctgcgcg cctactccat cgtcagcccg aactacgaag agacgctgga
     4021 gttcctcagc atcaaggtgc ccgacggccc gctgaccagc cgcctgcagc acatccagcc
     4081 gggcgacagc atcatcgtcg gccacaagcc caccggcacc ctgctgatcg actacctgct
     4141 gcccggcaag cgcctgtacc tgttcggcac cggcaccggc ctggcgccgt tcatgagcat
     4201 catccgcgac cccgacacct acgagaagtt cgatcaggtg atcctggtgc acggctgccg
     4261 cctggtgagc gagctggcct accgcgacta catcacccag gagctgcccc ggcacgaatt
     4321 cctcggcgag ctggtgagca gccagctcag gtactacccc acggtgacgc gcgagccctt
     4381 ccccacccag ggccgcattc ccgagctgat cgaaagcggc cgcatggcgc gcgatctggg
     4441 tctgcccgac ctgcacccgc agctcgaccg cgtgatgctg tgcggcagcc ccgagatgct
     4501 caagtcgatc aaggaagtgc tggaaaagcg cggcttcaag gaaggcaaca ccaccacgcc
     4561 gggcgacttc gtggtcgagc gggcgttcgt cgagcagtaa accggcctcg ccaccggccg
     4621 ggcgcgcgcc gcgtcagcgc ccgccggcgg caaaaaacgc caccatgcgc gccgtcgcgt
     4681 cctgagtcac ctgcgcgtcg tagacatagc ccctggccgg caaaccgggc ttgtcccagg
     4741 cgtgggtggc ttgcggatac agatgccact cgaccggctt gcccgctgcc ttcatctgct
     4801 ccagcaaggg aaagcaggca gacgcgggca cttctgcgtc gcgcccgccc atcagcatga
     4861 gcagcggtcg atcggtgtcc gacagcacca actggtattg tttgaacgcg cagctgccgt
     4921 agttggcgac cgtggccgca aagcgcagct cggagcccac cagccgcgcg ctctacggcg
     4981 aggccagatg ggtcgacacg aaagcccccc agctgtagcc cgcctgatag atgcgtgact
     5041 tgtcgacgaa cggcttgccc gccagcaggg caagtgccgc ataggcatcg gcgatgccgg
     5101 cggaatggct gagcacacgc gtggcgcaac tgtcgaagcc gcgcggaccg aagctgtcga
     5161 tcaccagcac cacgtgaccg gccgccagca gggactgcgc atgctgcttc atgtgtgcgt
     5221 tgggcgggcc cttcaggccg ccgcaggtat gcatcagcac ggtggccggg aacggcccgt
     5281 cacccgccgg cttgaacacc gtgttggcga cgttggtgaa caggccgatc gaatccttga
     5341 tttcatcgct gaacgcgacc ggcttgaact ccagcggctg cgccatctga gcttgcaggt
     5401 cctgcgcgcc gacgccccag tgaacgatca gcagcgcagt ggccaagagc aggcgatcaa
     5461 gaggtttgcg caggtgcatg acggtctcct tgtggtcagg tggtggcact cccgacgcca
     5521 tcgggctgcc cccacccagg cgccaggcgg cgtcttcagg gacggccgcg ctcgccgtgg
     5581 cggcgcatca ggtgcgccag caccggcggc gacacgaact gtgccacatc gccgcccagc
     5641 atgctgatct cgcgcaccag ggtgctgctg atgtgctggg tggcgctgct gggggtcagg
     5701 tagatggtgt cgacctcggg caccagggcg cggttcatgc cggccagctg cgcttcgtaa
     5761 tcgaagtcgg tcacgctgcg cacgccgcgc accatcaccc gcgcgccctg cgccacggca
     5821 aaggcggtca ccagaccggt gaagggcacc acgctgacct gcggcagatc ggccagcgcc
     5881 tcgcgcgtga tggccagccg ctcgtccagc gtgaacaggg tcttcttgtg gtgcgcggtg
     5941 gcgacggcga tgaccacgcg ctcgaacagc cgcgccgcgc gccgcacgat gtcctcgtgt
     6001 cccagggtga tggggtcgaa ggtgccgggg tagacggcga tgacgggctg gctcatgggt
     6061 gggctctccg gcgcggcggg cgctgtggcg gtgacacggt ggcaggcgat tatgcggaag
     6121 gcgccggccg ctgcagcagg tgggcgtgca ccgccccggc cttgaggtgg cggtgcagtg
     6181 ccaggcccag cgcgtccagc gcggcatcgt cccagcgttg cggcgtctcc aggtagacga
     6241 aaccgcccgg ccgcacggcg cgggcggcgg ccgtcagtgc cggcgccagc agcgccggct
     6301 gatcgaacgg cgggtcgagc agcaccagat cgacgctggc cggcgccagc gccgccagcg
     6361 ccgccacccc gtcgcccgcc tgcacgcgca ccatggccgc ccccaggcgc tgtttcagcg
     6421 cccgcaggcc ggccgccagg ccgtcgtcgc gctccaccag cagcacctcg gcagcgccgc
     6481 gcgaggccgc ctccagcccc agcgcgccgg tgccggcaaa ggcatcgacc acctgccagc
     6541 cggtcagcgt ctggcccagc cagttgaaca gggtttcacg cacgcggtcg ggcgtgggac
     6601 gcaggccggg ccgatcgggc accggcagca ggtggcggcg ccactggccg ccgatcacgc
     6661 gcacctgccg cggcagggcg gcgcggccac gggccgcggg tttcgagggc tgggacatgg
     6721 gcgcattgtc gcccgcccgt cggcgccggc gtgacgtgga tcaacaaagc gcgccagcgc
     6781 gggccgccat gcggccgccg ccggacactc ggaatagact agggcctgtg aacgctctgc
     6841 aaggggccgt cttgcagggc gttcgcaggc ccccaagcaa attcatccgc cactggacca
     6901 tcgccatgag cgccaagccc cagaacccgg tcgataccga cgcgccgctg accaacttct
     6961 cgcactgcca tgccggcatc ctgcggcatc tggacgactt cggccagttg ccggcgctgc
     7021 tggagccggc gcagcggctg cgccagatcg ccgagcgctt tgtcgagttc ttccgcgaag
     7081 cggtgttcga gcaccaccag gaagaagagc gcgagctgtt cccggccgtg ctggccagcg
     7141 ccgcgccggg cgccgagcgc gaacgtgtgc agcagatggt ggaccagctc acgcgcgagc
     7201 accgcgacgt cgaggcgcag tggaagcggc tggagccgca gatcaagaaa ctggccaagg
     7261 gccagccggc cgacgtcgac ccgcaggacg tcagaagcct ggtggcgcaa taccaggcgc
     7321 acgcccgctt cgaggagcag gagttcctgc cgctgtccga gcagatcctg tcgcgcaaca
     7381 gcgaccacat ggcggcgctc ggactgtcgc tgcacatgcg gcatcagccg gcgttcctga
     7441 gtcacatctg aattgctact ttttttgtag ctgctggcgc ttgccctgca aggggcaggc
     7501 gccttttctt tgcacaatcc gcgggccggg cgggtgccgc cgctcctgcg gcctggcgtt
     7561 caaaaccatg acccatgacc catgacccgt tggtcatcga agggcagcgt ggtcattggt
     7621 cattcttagc gcgcgttcgt atcagcgccc ggtaaagcgt gccgggcgtt tttcgatgaa
     7681 ggcgcgcacg ccctcggcgg cgtcctcgct ggcggccagg cggcgctgca ccggaccgaa
     7741 gtcggccacc gcggcggcct ggcccagttc gatcgccttg agcacgttca gtcgcgtggc
     7801 caccaccgcc tgcggcgcct gggcggcgat gcggcgcgcc agcgtcagcg cgctgtccag
     7861 cagttcggcc gccggcacca ccttctgcac gaagtggcaa cgcaaagcct cggcgctgtc
     7921 gaactggtcg gccgtcagca ggtgcagcat ggcgttgccg acgccggcac gctcggccat
     7981 gcgcagcgtg gcgcccccgg ccggcaggat ggcgcgctgc acttccatct gcgagaagcg
     8041 gcagttgtcg gcggcgatcg cgatgtcggc cgccagcatc agctcgatgc cgacggtgaa
     8101 gcagatgccc tgcaccgccg cgatcatcgg tttctggcgc cgccggtagc ccggcaggcc
     8161 cagctcgtgc ggctccacca ggccgtaggg ggtgagcggc tcgccgcgct gcaggtgctc
     8221 caccaccagg ggcagatcca gcccggcggt gaagtgttcg ccaaaggcgt gcagcacgcc
     8281 cacgcgcagc gtcgggtcgt cgtccagccg ggtgtaggcc tcggccagct cgcggaacat
     8341 gcgcggcgtc cagccattgc gcttggccgg acggttgagg ccgatcagca ggatctggtc
     8401 atcgatcacc tgggtgtcga tgcagccttc gggcggcggg gtgcggggga tcggggtggt
     8461 catgggtggg ctccttcagg gcaggctgga atcagatgat cgtcattcgt caccggtcat
     8521 tcccgttgcg ctggcccgct tccggcgccc cgaccaccac cgtgaccatg cgctcgggtt
     8581 gcagcttgcg ctgcagggcg gcgcggatgt cggccagggt gacggcggcc acgcgcgcgc
     8641 tccagtgctc caggtagtcc agcggcaaac cgttccaggc gatgttggcg acgttggcca
     8701 gcagcttgcg gttgccgtcc aggcgcagcg caaagccgcc gatcaggttg tcccgagccg
     8761 ccttcagctc gtcggcgctg gggccctggg ccacgaagtc ggccagcacc tggcgcacca
     8821 ccgccaccgc cgcgtcggcc tggtcggggc gggtctgcag gccgatgctg aaggccccgg
     8881 cgtgcaggcc gggcgcgaag tcgctgtaca cgctgtagga caggccgcgc tgctcgcgca
     8941 cttcgcgcat caggcgcgag gtgaagccgc cgccgcccag gatgtggttg cccaccagca
     9001 gggcaaagaa gtcggggtcg ctgcgcttgt agccgggctg gccgatcagc acgtgcgcct
     9061 gcgccgactg gaacgggatg cggatctcgc gcgccgcctg cagcggcgcc acctcggcca
     9121 ccggcggcag cggcgcacag gcgccggccg acggcagtcg ccccagcagc tcgcgcacca
     9181 gcgcgtcggc ctgcgcgcgg tcgaccgcgc ccaccaggct gacccgggcg cggcagggct
     9241 gcacatgctg gcgataaaag gcctgcaggt cggccacctg gatgcgcgcc agcgtctcgg
     9301 gcgtgctgtc ctggccgtag gggtggcggc cgtacaccgc ctgctggaac gccagcgccg
     9361 cctgggtggc cgggcgcgtg cgcgcttcct ggatggcggc cgacaggcgt gcgcgctcgc
     9421 gctgccacac gggctcgggc caggccggct cgcccagctc gcgcgcggcc agctgcaccg
     9481 cctggcgcag cagctcggca tcggtcagcg tgcgcagcgc aaagctcatg cggtcggcgc
     9541 cggcgctggc gccgaacgaa gcgcccaggt cggcccaggc ctcgccgagc tggttctcgt
     9601 ccagcgccgg ctgctcgccg cgtgcggcca cgcccttgcc ggccagcagc gcggtcgcgc
     9661 tggccaggcc ggcctgcgcc ggtgggtcgc gccggctgcc ggcgtcgaag tcgagctgca
     9721 cgtccagcat cgggatggcc gggctttcga ccagccagac ctgggcccca ctgggctggg
     9781 tccagtgctg gatcgggatc gccgcccacg cggataccga atgaaaaaag gctccagagc
     9841 ataccaggag cgcgcgaaca gctattgttt tcatagtctt catggcgctt ggcgcagaag
     9901 gtggcaaggg gttgcgaatg cccgaaggga gcgcccgacg ccgcccttca atggcgcgtc
     9961 ggcgcgggcg ccgggcgcgt ggcgccggcg gcgcgcggct gcggcaccag ggtggcgacg
    10021 gtgagctggt cgtcgccgaa atacttgcgc gccaccgcct gcacctgcgc cgccgtgacc
    10081 tggcgcagcc gcgccaccag gcgctggtcg gcgtccagcg gcaggccctg cacccacagg
    10141 tggcccagct cctgcgcctg gcccatcacc gagtcgcgct tgtagacctc gctggccacc
    10201 cactgggtct tgacgcgcgc cagctcggcc gggctgacgc cctgctcggc cacgcgcgcc
    10261 acctcggcac gcagcgccgc ctcgacctgc gccgcgctct tgccggtggc cggcacgccg
    10321 ccgagcgtga accattgcgg cccgcgcccg agcaggccgt tgccggcgct ggccgaatcg
    10381 gccacgcggt ccgggccctg ggtcagcgcg cgctccagcc gcgcgccgct gtagccgtcc
    10441 agcacggcgg ccagcacggt gagggccagc gcgtcgtcgt tgtcggcgcc cggctccagg
    10501 ccgtcgagcc gcggcacgcg aaaggccagc gacaccacgg cctgctcggc cggcgccttg
    10561 acctcgatgc ggcgcaggcc gagttgggct ggctcggggc gcggcttgcg cccgggcagc
    10621 gggcgcgccg ccatgccgcc gtagtggcgc tgcgccagcg ccagcacggc ggccggctcg
    10681 acgtcgccga ccaccaccac ggcggcgttg gccggcacgt accactggcg atggaaggcg
    10741 cgtgcgtcgt cggccgtcat ggcctgcagg tcgcccatcc agcccaccac cgggcggcgg
    10801 tagggcgagg cgatgaagcc gaccgcgcgc tgctgctcga acagcagcgc gcgcgggttg
    10861 tcctcggtgc gcaggcggcg ctcttccttg accacctcca gctccttggc gaacacctcg
    10921 tcgctgtagc gcaggtgggc gaagcgctcg gcctccagcg ccatcacctc gcccagcctg
    10981 gccgccggca cctgctggta gtagccggtg tagtccaggc tggtgaaggc gttctcgcgc
    11041 ccgcccagct gcgccacgcg gcgcgaaaac tcgcccggcg ccagccgctc gctgcccttg
    11101 aacatcatgt gctccagcac atgggccacg ccgctggtgc cgtccacctc gtccatggcg
    11161 cccacgcgca gccacagcat gtgcaccgcc gtcggggcgc gacggtcggg tttgacgatc
    11221 accgtcaggc cgttgtcgag ggtgaagcgc tgcaccgcgc cggcggcggc ggccggcgac
    11281 gctgcatcgg tggcggccgg cggcggcgcg gacgcgcagg cgcccagcag cgcggccagc
    11341 agcacaggca gcaggaaagc gggtctgaag ttcatgtcgt cgtgcagaga tcggttcggc
    11401 ggcccggatt ccccgcccgc ggccggcagc ggcgattgtc gccgcgccgc cggggggccg
    11461 cggctgccac aatggcggcc catgaactcg cccgccgccg cccccgccac gcccaccccc
    11521 gaaccgcgcc tgacctcgct ctcgcatggc ggcggctgcg gctgcaagat cgcccccggc
    11581 gtgctgtcgg ccatcctcaa ggacaccctg gccatgccga tgccgcccga gctgctggta
    11641 ggcatccaga ccgccgacga cgccgccgtc taccgcctca acgacgagca ggcgctggtg
    11701 gccaccaccg acttcttcat gcccatcgtc gacgacccgt tcgacttcgg ccgcatcgcc
    11761 gccgccaatg ccctgtccga cgtctacgcc atgggcggca cgcccatcat ggcgctggcg
    11821 ctggtgggca tgccgatcaa cgtgctgtcg ctgcagacca tcggccgcat cctggaaggc
    11881 ggcgccagcg tctgtcgcga cgccggcatc ccggtggccg gcggccacac catcgactcg
    11941 gtggagccga tctacggcct ggtcgccctg ggcctggtgc accccgagcg cgtcaagacc
    12001 aacagcggcg cccaggtggg cgaccggctg gtgctgggca agccgatcgg cgtcggcgtg
    12061 caatcggccg cgctgaaaaa gggtgccctg ccggccgccg gctacgccgc catgctcgcc
    12121 agcaccaccc agctcaaccg tgccggcccg gcgctggcgc ggctgccggg cgtgcacgcc
    12181 atcaccgacg tcaccggctt tgccctggcc ggccacgcgc tggagctggc gcgcggcgcc
    12241 ggcgtgacgg tgcacatcga ctggcccagc gtgcccctgc tgcctggcgt gggccaactg
    12301 gccgcgcagg gcatggtgac cggcgcctcg gggcgcaact gggccgccta cggacacgac
    12361 gtggcgctgc cggccggctt tggcgacagc cagcgcgccc tgctgaccga cccccagacc
    12421 agcggcggcc tgctggtggc ctgcgacgcc gccagcgtgg acgaggtgtt ggcgaccttc
    12481 cggcacagcg gcttcgcgca ggcggcggtg atcggccgca tcgagcctgc cggcgcctcc
    12541 ccgctggtgg tggcctaggg cctgttaacg ccgaggggcg ggctgtgccg agcttccctg
    12601 cgggttgcgc cgcaaaggaa ccgtctgcgg cgttgcccgc gcttgcaagg cacacgcctt
    12661 ggctggcgca cgggcgcctt gcatccagcc cctttgggaa gctacgcgat ccgaatgcat
    12721 agcgtaagca ggccctgagc cgcgcagcgc atcagggcat tccccagcag cgggccgggg
    12781 ccggcgtgcg catactgcgc gcccatgacc cagcagctgc cgcgccccca cgccacgccg
    12841 gccgtcgcgg gcggcattcg cctggggcag gcgacagcga cggtcaatga agcgctgcag
    12901 ctgcgccgct atctcctcgc actggggttg accgaacatg tgacccgcca ggacggcatc
    12961 tggcggcttg accccgaagc cgcgcaacgc tggctgcccg caggcgacac cctggggctg
    13021 caccagcggc tggcggcacg ctggcagccc gatgcgctgg agctgcgtcg gcagatcgtg
    13081 gtggccatgc tggccggccc ctgggtgttc gagtttccgt cctgcgccga actgcgggcc
    13141 gccgtgcgcg tgcgcgaacg catcgtgcgg gccgctcgcc agaccgccct ggcgttccgc
    13201 accgaggccg ccgaaagacc ggccgaagac tggtgctacc acccggacac cggtttcacg
    13261 ttgcgccccg ggcgcggtct gatcgcggcc ctgcgcaagg ccacccagcc ccgggtctcg
    13321 ggccgtctgt acgatttttc ctgctatcgc gccaccgaat acgtcatcct gctggccctg
    13381 gcccaggaag cggcgcgcag caacccggcc ctgctggcac gcctgcagcg tcagtgggaa
    13441 tgcaaggcca tcatgtcggg cagctttcac gacaccttcc tgcgcgaata cggccatcag
    13501 gacaccccgt tgccgacgcg ctactacgtc cccggcgacc ggctgtggtt tcgcaacccc
    13561 gacgaagcct ccgccgacgc caccgggtac gaaggctcct gggtgttcta cctgggccat
    13621 ggcgagttca gcgacttctg gcgtgccggc agaacctaca cgctggagag caagtgtctg
    13681 gagatctacc actggcgcga cggcctgtac cgcgacgccc gcaccggcgc cagccagatc
    13741 gacgaaggcc gggtggcaca gcaggtggcc gccacgctgc gtgaccctgc cgggcacgca
    13801 cgcattcagg cgtcgatgca gcgctaccgc gaacccaggg gctgctacac cgacgccggc
    13861 ggctgcatcg acaccacgcg cgaatacccg cgctgcatct gccccgccga ctgcgagatc
    13921 gacctgcccg acgccccgat ctgacctgca ccacgcatgg gctgcctaga atcggtccat
    13981 gttcagcttc ttccgccgca agaaagccgc cgagccggca cccgaaaacg ccgtacccga
    14041 ggccgcaccc gccgacccgg cggccgaact ggccgaagcg gccacgcccc tgagggcgga
    14101 cgaactggaa gcccaggcgc tggccgccgc gcccgaggag gggccacatc cagcgccctc
    14161 ggttgctatc gaaacagaag ctgctggcgc agcacccaca agcgctgagg tcgaatttga
    14221 tcatgaatcc gcgaacctgg cgcagcccac ccccgccgcc cgcagcggct ggctcgaccg
    14281 cctgaagagc ggcctgaaga aaaccggcag cagcatcacc accgtcttca ccggcacgcg
    14341 catcgacgag gcgttgtacg aagacttgga aaccgcgctg ctgatggccg acgccggcgt
    14401 gccggccacc gaaaccctgc ttgccgacct gaagcgccgc gtcaaggagg cgcgcgccac
    14461 cgagccggcc caggtcaggg cgctgctgca ggacgccatc gccgacctgc tggcgccgct
    14521 ggaaaagccg ctggtggtgg ggcagtacca ccccaccgtc atcatggtgg ccggcgtcaa
    14581 cggtgccggc aagaccacca gcatcggcaa gctcacgcgc cacctggccg aggccggcgc
    14641 cacggtgctg ctggcggccg ccgacacctt tcgcgcggcg gcgcgcgagc agctggcgat
    14701 ctgggccgac cgcaacgcgg tcgaaatcat cagccagcag ggcgccgacc cctcggccgt
    14761 ggccttcgac gccgtggccg ccggccgcgc gcgcggccgc gacgtggtgc tggtcgacac
    14821 cgccggccgc ctgccgacgc aaaagcacct gatggaagag ctggccaaga tccgccgcgt
    14881 ggtcaccaag gccgacgtca ccgcgccgca cgaagtgctg ctggtgatcg acggcaacac
    14941 cgggcagaac gcgctggcgc aggtgcgcgc cttcgacgag gcggcccagc tcagcgggct
    15001 gatcgtcacc aagctcgacg gcaccgccaa gggcggcgtg ctgtgcgcca tcgcgcgcga
    15061 aaagccggtg ccggtgtact tcatcggcgt gggcgagcgg ctggaagacc tgcagacctt
    15121 ccgcgcacgc gagtttgccc aggcgctggt cggctgagcc gctctgaacg cgccccccag
    15181 gcccaccttc tgtgcgccgc ccgctgcaac cgaatgcaaa caccggcgtg cccccgcgcc
    15241 ctgtcgatac actcgggcgc atgagcgtca atcccccccg gatacacctg gtgctgctgg
    15301 gcgcgggccg tgcccacatg caggtgctgg gctcgctggc gcgccagggc agtggacatc
    15361 tggcggtcac cctggtggcc ccgcacccct actacatcga acgcgccatg ctggccggct
    15421 acgtggccgg cgactacgcg ctggaacagc ttcgcctgcc gctcgaccgg ctgatcgaac
    15481 gcagcggcgc cagcttcgtg ccggcgcatg tgatcgcgct cgaccccgac gggcgccgcg
    15541 tgcagctgtc cagcggcgac gccctgccct acgacgtgct gtcgatcaac ctggagccgg
    15601 cgctggagcg cgagcgcatc gaacaggcca tgcccggcgc ccgcgccaac gcgctgttca
    15661 cccatccgct ggagaccttc gtgcacctgt ggccgcagct gctggcgctg gccggcgagc
    15721 gcgcgctgca ggtggcggtg gtgggcgacg gcctgccggc ggtcgagctg gccctggccg
    15781 tggcacacgc agtggcggcg ccgcacggca gccgcgtcac cctggtggcc ggcagcgcgc
    15841 cgctgctgac cgacccgtcc agcgcgctgc agcggcgcct gctggcgcgc ctgaagcagt
    15901 cgcacatcac gctgctgcac gaccagtgca ccggcatcgc cagcggcgtc atgcaactgg
    15961 ccagcggcgc caccctggcc tgcgacgcgc cgctgctggc caccggcggc ggcacgcccg
    16021 gctggctgat gcaggccggg ctgctggcca acgacgccgg cgaaccgctg gtcaacgaac
    16081 ggctgcagag cgacagccac cgccagatct tcatcgtgcc cgacgacgca ccggccgaag
    16141 tcggcccggt gctcgacgcc aacctgcgca ccgccgtggg cggcggcagc ttccgcaagg
    16201 cccccggcga gccgccgcgc ctgcgcatcg tcagctgcgg cagcggccag gcggtggccg
    16261 cctggggccc gctggggctg gaaggccgcg aagtctggca ctggaaggac cggcgcgacc
    16321 ggcgccagct ggcggcgctg tttgccgtct gatccgaccg ctgcatcacg aatgacctcg
    16381 ctgcgcttgc atggcgccct cacccagcag gtgaaggctc cgaagtggcg caaggtcaga
    16441 gttcatgcgg cattcccaag ggttgcaagc ggtcaaccgc cgtttctagg aggctgtcgg
    16501 gcttggagcg ccgaaagcga gaatcggccc cagcgagacc agtttttgcc ggatttgcag
    16561 cccaataggt gagctattgg gcaaaaagcc ggtgaaaaat gggccgttgc ggtcgatttg
    16621 cagccggcga tctccaagtc cgacagcctc ctaggttcat agcatctggc gcagctcgcg
    16681 cgcggcttcg cgcaggcgtg gcagcagctc ctgctgcatc agcgcgtcgc tgaagcgcgc
    16741 cggcggcacg atgacgttga gcgccgccac ggtggcgccc tgggcgttgc gcagcggcac
    16801 cgccagcgcg tgcacgccga gctcgttttc ctcgctcgac agcgcccagt cctggcgcgc
    16861 cacctgcgcc agcagcgcct tgaaggcgcg cgtgccggtg gtggtgtgcg gggtcaggcg
    16921 cggcagcgcc tggtggtgcg ccagccagtc ggtcagcgcg ggccggggca gcgccgccag
    16981 cagcacgcgc ccggtggacg tggtgtgcgc cggcaggcgc gcgcccaggt gcatgccgta
    17041 ggccagcagc cgcgtgccgg gcgcccccag gccggcgctg cgggcgatga tcaccacctc
    17101 gtcgccgtcc agcaccacgg ccgaatacac gtcgtgcgtg gccgccgcca ggcggttgag
    17161 cgccggctgg acgacgcgcg gcagccgcgc cgacgccagg taggcgccgg ccagccgcag
    17221 cgcgcgcggg ctgagccaga agaactggcc gtcgctttcc aggtagccga catgggccag
    17281 cgtcagcaga tggcggcgcg cggcggcgcg cgtcaggccg gcgcgctgcg cggcctgggt
    17341 ggcgttcagg cgctggcgtt cggtgtcgaa actctccagc accgccagcc ccttgacgag
    17401 gccggcaatg gtgtccgcag gcgccaggtg cattgcgcga tgatcgcaca gtttgcgcgg
    17461 tgatcgcgca gcggtgcggt gcccgcactg gcggccggcg ccgccggcgc ctaagctcac
    17521 ggctttcccg ccttcacgca cgaggagaca caccgtgacc accacccgca cccaggtagc
    17581 catcgtcggc gccggcccgt ccggcctgct gctcggccag ttgctgcaca aggccggcat
    17641 cgacgccgtg atcgtcgaac gcgtcagcgg cgactacgtg ctgggccgca tccgcgccgg
    17701 catcctggag caggtctgca tcgacctgat ggacgaagcc ggcgtgggcg cgcgcatgca
    17761 ccgcgaaggc ctggtgcatg gcggcatcga gatgctgtgg gacggccgcc gccaccgggt
    17821 ggacctgaac cggtacagcg gcggcaagaa cgtgatggtg tacggccaga ccgagctgac
    17881 gcgcgacctg atggacgcgc gcgccgccgc cggcctgacc accgtctacg aagcgcacca
    17941 caccgcggtg cacgacttcg acacggctcg gccgtacgtc acctacgtga agaacggcgt
    18001 cacgcaccgc atcgactgcg acttcatcgc cggctgcgac ggttttcacg gtgtgtgccg
    18061 cgccagcgcg ccgcgcagcg ccatccgcga atacgagaag gtctacccgt tcggctggct
    18121 gggcctgctg tcggacacgc cgccggtctg cgacgagctc atctacgtca actccgagcg
    18181 cggttttgcc ctgtgcagcc agcgcagcaa gacgcgcagc cgctactacc tgcaagtgcc
    18241 gctgacgcac cacgtggaag actggagcga cgacgccttc tggcaggaac tgcggctgcg
    18301 cctggacgac gaggcgcgcg agcgcctggt caccggcccc agcctggaaa agagcatcgc
    18361 cccgctgcgc agcttcgtca ccgagccgct gcgttttggt cgcatgttcc tggccggcga
    18421 cgccggccac atcgtgccgc ccaccggcgc caagggcctg aacctggcgg ccaccgacgt
    18481 caagtacctg ttcgacggcc tggtccagca ctaccgcgag cgcagcgacg ccggcctgga
    18541 cgcctattcg gccaaggccc tggcccgcat ctggcgcgcc gagcgcttct cgtggtggtt
    18601 cacccagctg atgcaccgct tcccgcacga cgagcagatc accgccaagt tccagcacgc
    18661 cgagctggac tacctgatgc attccgaagc cggcctgcgc accatcgccg aaaactacgt
    18721 cggcctgccg ctggacttcg gccagcgctg acgcgcccca cggccggcca aggccagcgc
    18781 ggccaccagg cgcgagcctg ggtcggcgct cgcgcgcatg gcaggcggcc ggccgccgcc
    18841 ttcgggttca aggcaaaatg gcttgcggcg cgccaccgtg gtgcgccagc agctatcact
    18901 ttgcaagtcc atcccatgcc tgtacctgcc cccatccgac ccctgcgcgg cacgcgcatc
    18961 gtcagcctgg cgctcaacct gcccggcccc gcggcgctga tgcgcctgca cgcgctgggc
    19021 gcgcgctgcc tcaaggtcga gccgcccggc cccggccgca cggcggccgg gcggccgatt
    19081 ccgggcgacc cgatgggcca gtacgacgtc ggcgcctacg gcgtgctgca ccagggcatc
    19141 cgcaccgtgt cgatggacct caaaagcgag cgcgggcgcg cccggctggc caccgagctg
    19201 gcgggcgccg agctgctgct gacctcgttt cgcccttcgg cgctggccaa gctgggcctg
    19261 ggctggcgcg cgctgcaccg cgcgtacccg acgctgtcgc tggtggaaat cgtcggcgcg
    19321 ccgggcgagc gcgccgaaga gcctggccac gacctgacct acctggccga agccggcctg
    19381 gtcaccggca ccgagctgcc ccccaccctg ctggccgaca tggccggcgc gctgctggcg
    19441 gtggaggccg cgctcgccgc cctgatgcag gcgcgcgcca gcggccgcgg cgtgcaccgc
    19501 caggtggccc tgtcggacgc cgcgcactgg ctggcgctgc cgcgcaactg gggcctgacc
    19561 ctgcccagcg gcgccgtcgg cggcgcgcac gcgggctacc gcgtctaccc ctgcaaggac
    19621 gggcgcgtgg ccatcgccgc gctggagccg cactttgcct ccgcgctgtt caaggtggcg
    19681 ggcctgcccg cgcccgacct gcgcgccccc ttggcgccgc aggcgcgcga cgccatcgcc
    19741 gcctgggcgc tgggcaagac gcgccagcag ctcgacgcgc tggcgcgcca gcacgacatc
    19801 ccgctgcaca ccctgccgcg cgcctgagcc ccatgcgcgg cgaaggcagc atccggcgct
    19861 gggatgcggc gcgcggcttc ggcttcatcc agaccgccga gcggcgcgac gtgttcttcc
    19921 acgtgcgcga cttccgtggc gccggcgcgc cgaccgaagg cctgcgcgtc agcttcgaag
    19981 cgatcgaggt cggcggcaaa ggcccgcgcg ccatggacgt gcggccactg tcggccaccg
    20041 ccggcccggc cgcgacgacc ccggcggcgc gcccgcagcc cacggcgccc cgcagcgcca
    20101 gcgccacggc cgcgcgtgcc cggccggagc aggtgcccag ccgggcgcac gccggcccgc
    20161 cgcccgcccc gcacggcgcc gcgctggcct ggctgctgat gctgggctgg ctgggcctgc
    20221 tggcggccgg cgcctggtgg caacgcctgc cgacctggct gttgccggtc ctggtggtgc
    20281 tcaacctggc cacctacctg gcctacgccg gcgacaagcg cgccgcgcaa cgcggccact
    20341 ggcgcacccc cgagcagacg ctgcacctgc tggccctgct gggcggctgg cccgccgcct
    20401 ggtgggcgca gcagtggctg cgccacaaat cgcgcaagct cgaattccgc ctcgcctact
    20461 gggtcaccgt ggtgctgaac tgcgccgccc tggcgctgct ggtggcgcgc cccacgtggc
    20521 tgcccgcacc ctgacgcccc acctatgatg tcacccggac gcgcgccacc gggaggaccc
    20581 tttgaacaac cacctgcacc cccaccagaa acaggagctg gccgagctgg cggtgatcgc
    20641 catgcgcgag cgcgggctgg cgcccgagtt cggcgccgcc gtgcgccagc agctcgacac
    20701 catcgacggc ccggccgaag acgaaggccc cgacatcgac gacctgaccg ggctgctgtg
    20761 gtgctcgatc gacaacgacg attcgcgcga cctcgaccag ctcaccgtca gcgaaaccct
    20821 gcccgacggc ggcgtgaagc tgctggtggc catcgccgac gtcgacgccc tggtgagcaa
    20881 ggacacgccg atcgaccgcc acgcgcagat caacaccacc agcatctaca cctcggcgcg
    20941 catctttccg atgctgccgg aaaagctcag caccgacctg acctcgctca acccgcacca
    21001 ggtgcgcgtc gccaccgtca ccgaaatggt gttcgccccc gacggcacgc tgctgcgcag
    21061 ccacatccgg cgcgcgcgcg tgcgcaacca ggcgcagctg gcctacgacg ccgtgtcggc
    21121 ctggctcgaa ggccagggcc cgctgcccga agcggccgac cgcgtgcccg gcatggacga
    21181 ccagctgcgc acccaggacg cgctggcgca gcagctgcgc gccaaccggc gcgagcaagg
    21241 tgcgctggag ttccagaccc tgcagccacg cgccgaattc gaaggcgagc gcgtggtcgc
    21301 catccgccag caggagcaga accgcgcgcg ccagttgatc gaggagttca tggtcgccac
    21361 caacggtgtg acggcgcgct ttctggccgg caagcggcgc gccgccatcc gccgcgtggt
    21421 gcgctcgccc gagcgctggg cgcgcatcgc cgaagtcgcg gccgagcgcg gctggcgcct
    21481 gccaccggac cccgacgcgc acgcactgga gcagttcctg gccgccgagc gccggcgcga
    21541 cccgctgcgc tttcccgacc tgtcgctcgt catcgtcaag ctgatggggc gcggcgaata
    21601 cgtggtcgag cgcccgggcg ggccggtgat cggccacttc gggctggcgg tgcgcgacta
    21661 ctcgcactcc accgcgccca accggcgcta ccccgacctg atcaccggcc ggctgctgaa
    21721 ggccgcgctg gcgggcgagc ggccgcccta ctccgacacc gagctcgaat tcctggccaa
    21781 ccgctgcacc agccaggaag acgcggccaa caaggtcgag cggcgcctgc gcaagtccga
    21841 agccgcgatg ctgctggaag gccagatcgg caaggtgttc gacggcgtgg tgaccggcca
    21901 gtccaagggc agcacctggc tgcgcatctt cgacccgccg gccgacggca agctgctggc
    21961 cagccgcgaa ctgaaggtcg gcgacaaggt gcgcgcgcgc ctggtgttca cccacgtcga
    22021 gcacggcttc atcgacttcg agcagatcga ctgaccccct ggccaacgcc ggccgcgcgc
    22081 tgcacaatgg ccgcacctgt ggcgaggagg ccagcatggg cagcgtgatc agcttctaca
    22141 tcgaccagga cagccccggt tttcgcacca ccggcctgct gcccagcccc gaggacgaca
    22201 tcgcaccctg cgcgctgagc gtgggcgaca ccgtcagctt tccgaaccac cgcaatcgcc
    22261 gctaccgcgt ggtcagccgc cattgcaacg ccggcgccga catggacgag ccgcagtggc
    22321 tgctgcggct cgaaccgctg cgctgacaga tcctgttttt atagctgccg gcgcttgcca
    22381 gacaagcgcc ggcgccattt tttgcttgca aatggcgatg ggccctgggg ccgcctccac
    22441 cgcttcgcgt tcaagcccat gacaccggcc gcaccggccg gacccacgac cccttggtca
    22501 tcgaagcgca gcgaggtcat gcgccatgca gcgctcgcat cagaaccccg cccccggctg
    22561 cgccagatag tccagctcgg ccgccgtgct ggtgcgcccg aggatggcgt tgcggtgcgg
    22621 aaagcggcca aagcgcgcga tcacgtcctg gtgccggagc gcgtagtcca gcgtgccgtc
    22681 gaagaacgcc cgcggctcgg cgccgggcgc gtcgcgcagc gcctgaaaca gcgccacgtt
    22741 gcgcgcctgc agggtggcgt cctcggcgtg ctccagcggc aggtagcaga agatgcgcgc
    22801 catcggcggc accgcctggt cgtgccccag ctcgatgccg cgcagcgcca gggccagcgc
    22861 ctgcgcgtcg ccggcaaaac cgtcgggctg gccgcgaaag gcgttgcggg tgaactggtc
    22921 aagcacgatc agcagcgcca ggcgcccttc ggcagtgtcg gcccagcggt cgagccgccc
    22981 ggcgcgcgcc gcggcgatgg tggcgccaaa gccttcgcgc aaacgggcgt cgaaggtctc
    23041 gtccttgcga aaccactggg cctgcacgcg ctgcatggcg gcgctgtcca gtgggtgggc
    23101 gccgagccag aagcgcagca cgtcggaggc ggatcgggtc atgccggcat tgtggcgccg
    23161 acccgaccgg cccgctgacc ggcatcagga aatgcccgca cgccccttgt gccgggccgg
    23221 ccgccacact acgctgcgag aaaccgagga gacccagagc catgcccatt gccgccccca
    23281 ccgtcgcaca gatccgcaac ctggccctgg tcgggcccgc cgccagcggc aagaccacgc
    23341 tggccgaagc cctgctgcac cgggccggcg ccatcggcgc catgggcagc gtggagcgcg
    23401 gcagcaccgt gagcgaccac gacccgcagg agatcaaggc ccagcattcg ctgcaatcgt
    23461 cgctgctgca cctggaccac gacggttgcc gcgtgcacct gatggacacc cccggcctgc
    23521 ccgatttcat aggccagagc ctgccggcgc tggaggcggt ggagacctgc gccgtcgtca
    23581 tcaacgccgc caccggcatc gagcccatgg cccggcgcat gatggactgg gccgccgagc
    23641 gccagcagga ccgcctgatc atcgtcaaca agatcgacgc cgaaggggtg gacctgcccg
    23701 ccctgctggc cgacatccag gcccagttcg gcagcgaatg cctgccgctc aatctgcccg
    23761 acgccggcgc gaccaaggtg gtggattgct tctacaaccg tgacggccac agcgacttcg
    23821 gcgccgtggc cgacgcccac cgcgcgctgg tcgagcaggt ggtcgaggtc gatggcgact
    23881 tcgtcgagcg ctatctgaac gacggcgaca tcgacgccgc cgagctgcac gccccgctgg
    23941 agcaggcgct gcgcgagggc cacctgatcc cggtctgctt cgtctcggcg cgcagcggcg
    24001 ccggcgtggc cgagctgctc gacatcatca cccgcctgct gcccaacccg atcgagggca
    24061 atccgcccga cttcttcaac ggcgaaggcg aggccgccac cctgatgcac gcccgccccg
    24121 acccggcggc ccatgtgctg gcgcaggtgt tcaagatcag catcgacccc tacgtggggc
    24181 gcctggccta tgtgcgcgtg caccagggca ccatcacgcc ggcgtcgcag ctgttcatcg
    24241 gcgacgggcg caagccgttc aaggtcggcc acctgttcct gcagcagggc aagaaccacg
    24301 tggaagtgcc cagcgccggg ccgggcgaca tctgcaccat aggcaagatc gacgagctgc
    24361 actacgacgc cgtgctgcac gacgccgccg aagacgccca cctgcacctg cgcccgctgc
    24421 catttccggt gccggtgcac ggcgtggcca tccggcccaa gaagcgcggc gacgaacagc
    24481 gcctgtggga aatcatggcc aaactcgtgg ccgaagaccc ctgcctgaag atcgagcgcc
    24541 tggccagcac gcacgaaacc gtggtctacg gcttgggcga gctgcacctg cgcctgctgc
    24601 tcgaacgcct gcgggacggc tacaaattcg aggtcgacac ccagccgccg cgcatcgcct
    24661 accgcgaaac catcgcccag aacgccgccg cgcagtaccg ccacaaaaag cagagcggcg
    24721 gcgccggcca gtttggcgag gtgcacctgc gcgtcgagcc gctggcgcgc ggcgccggct
    24781 tcgagtttgt cgaccaggtg aagggcggca ccatccccgg ccagttcatg cccgcggtgg
    24841 aaaaaggcgt gcgcgaggcc ttggccgagg gcgtcatcgc cggctacccg gtgcaggacc
    24901 tgcgcgtgat cgtgcacgac ggcaagcacc acccggtgga cagcaaggag gtggccttcg
    24961 tcaccgccgg caagaaggcc gtgcaggccg ccatccgcga ggcccggccc atcgtgctcg
    25021 aacccatcgc ccgcctggcc atcaccatcc ccgacagcgc caccggcgcc gtcaccggcg
    25081 acctgtcgtc acgccgcggc atcgtcagcg gcaccgacag cccgcgcccc ggccaggtca
    25141 gcgtcaccgc ccaggtgccg atggccgaac tggccagcta ccagatgcgc ctggacgccc
    25201 tgaccgccgg ccagggcgcc tacaccctgg ctctgtccca ctacgaagcc gtgccgccgc
    25261 aggtgcagca gcagctggcg gggcagtaca aggtcaagga agaggagtga caggcgacgc
    25321 gccggcgtgg cgctggcgct gattggccac attcgtgaac gaatgctggg tgaggtcgcc
    25381 cagcgctgcg gtatagtccg attgagcaat tggttacata tggactgccg cttgcccagc
    25441 caccatcaac cgatccgccc tgttttaggt ttccctaaac atggcgtggc ggggttctcc
    25501 atggcgtttg cgcgtttgga gttgcgttgg gtgacggcgg atcagatgta ccttgtcgtc
    25561 taattctagt tagccttgca aaatacccgt gctttattaa gcaaatgatt tacaagacgg
    25621 cct
//
LOCUS       DAOQTZ010000032        25372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2278_length_25372_cov_13.456729, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000032 DAOQTZ010000000
VERSION     DAOQTZ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 25372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25372
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_25372_cov_13.456729"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1351
                     /locus_tag="PKN51_05590"
     CDS             98..1351
                     /locus_tag="PKN51_05590"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019478 - D-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNT84607.1"
                     /translation="MNIAILGAGIVGVTTAYELAADGHQVTVFERHASVAEAASFANA
                     GVVAPGYVTPWAAPGMPLKVLRYLLAHHAPVRLRRPLALADIAWMRHWLAACEGTAYA
                     RNRARMQRLAFYSRHRLHRLADAMAYDFDRSSGYMVLLRAERDRRLAQPGLALLRELG
                     VPFDELDADAARRIEPALHPHTALAGAIHLPQDEVGNCRQFAIILRDAAEQLGARFAF
                     NTTVTAIDKTQPATFHVAHSAVPLRFDALVLCAGVGSAELLRPLGLRLPLRPVYGYSV
                     SAPVPEPARAPRSGVMDERYKVAITRMGQRVRVAGSAEIGGRPDDVHHAALQTLYKVL
                     TDWFPGAARLSSGVQVWKGARPMLPDGPPVIGASGVPGLWLNLGHGSSGWALSCGSAR
                     VLADQIAGRVPDIDTEGLDVQRWLR"
     gene            1387..2928
                     /locus_tag="PKN51_05595"
     CDS             1387..2928
                     /locus_tag="PKN51_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNT84608.1"
                     /translation="MPDLLPFDRPAPLFDLAATRRIEATAQAALPPHTLMQRAGLATA
                     RLALALAPHARTVWVACGPGNNGGDGLEAAMHLQGWGLHPAVTWLGDEAHLPADARAS
                     LQRARAAGVGFADAPPPLGPHDLAIDALLGIGATRAPSGRMADWLRQLRHGPATLLAL
                     DVPSGLDADTGCLHDSDATHSIAVGASSACAGGRFDTQPPHGARHTLSLLTRKPGLFT
                     AQGRDACGTLWFDDLGVDPGEVAPTAWLNAPPALAPRPHDSHKGRFGDVAVIGGASDT
                     AQGTAMTGAALLAAAAALHAGAGRVYVGPLGATAPTFDPVRPELMFRPPDRLPLAQLT
                     VVCGCGGGDAVRAVLPAVLAQAPRLVLDADALNAIAQDAALQQALAARAGRGQSSVLT
                     PHPLEAARLLGGDVRQVQADRLRAAQALAGRLESTVVLKGSGSVIAAPGQTPRLNATG
                     NARLATPGSGDVLAGLIGARLAGGRPALEAACDAVHQHGALADAWPAHTALTAGALAQ
                     RLTPA"
     gene            2957..3625
                     /locus_tag="PKN51_05600"
     CDS             2957..3625
                     /locus_tag="PKN51_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT84609.1"
                     /translation="MRILLAEDEPELASWLARALERGGLLADWVSDGRDVLPSLAARR
                     YDALVLDLGLPGLDGHQVLAALRATDQRLPVLILTARDSLIERVGTLNQGADDFLAKP
                     FDLAELEARLHALIRRARGSAHWRQSCGPLAWDATTRGFTLNEAPLTLTPREHAVLRV
                     LIQHVGEPLSKAEILDRVVSDERDVNPEAIEVLVHRLRKRLAGSQVRIGTLRGLGYVL
                     EHGA"
     gene            3622..5073
                     /locus_tag="PKN51_05605"
     CDS             3622..5073
                     /locus_tag="PKN51_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HNT84610.1"
                     /translation="MRLGWLRASLRRALALVLAAGMLLASGVGLWGAWRSAAGAANAA
                     YDRSLAGAIKAIDANITTAGGGLGVELPYTLLEFFQLTAGGQVFFRVATEDGLVEIGN
                     TDLPPPDTGLINGRPRFRDALYHGQPVRLGSYTRVLDPPPAGHTGRPRVVIQVAETLT
                     SRQDFTRALLLEAVGRNALLVTLALLLMAWATGWALRPLARVSREVQARPPDDLTPLP
                     LADVPAEARPLVEAMNHHMARHRQASEARRRFVDDASHQLRTPLTTLSTQLAYALREA
                     PPGPLREALTAMRAQLDGATRQVNQMLALARADAAEPRAEVVDLVALARELTRHGWPR
                     AREAGIDLGFESQAEQLPASVQPILLQEALGNLLHNALRYCPAGSRVTVRVAQEGGQA
                     RLTVLDDGPGLAPDELARAGERFFRGRQAAAGGTGLGLAIARAVAERHGGTLSLQTGA
                     GGCGLAVSLLLPLRELPTSPGFPQVETAPLKAD"
     gene            5161..6141
                     /locus_tag="PKN51_05610"
     CDS             5161..6141
                     /locus_tag="PKN51_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT84611.1"
                     /translation="MKFRLPLAALTLACGLGLAHAGPLDKTECIAPAKPGGGFDLTCK
                     LAQSSLLNGKYISDPMRVTYMPGGIGAVAYNAVVAQRPAEGNTIVAFSGGSLLNLAQG
                     KFGRYGENDVRWLAAVGTDYGAVIVAEASPFKTLNDLVAAIKADPTKVVFGAGGTVGS
                     QDWMKAALTAKAAGLDPRKMRFVAFEGGGEASTALQGGHVQVYSGDASEAEEQIKAGA
                     KIRVLAVMADKRLEGGLAKVPTAKEQGLDVEWPIIRGYYMGPKVSDADFKVWTDTFSK
                     MMATPEFAKARAERSLYPFAKTGADLDAYVKQRVADYRKLAAEFGLNVTK"
     gene            6197..6643
                     /locus_tag="PKN51_05615"
     CDS             6197..6643
                     /locus_tag="PKN51_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNT84612.1"
                     /translation="MSDRILGVACVVVAAGMAWAAKDYAAPISYEPVGPRAFPLLLAT
                     LMAAGGLWLIVRPNLRGNWLRQVPLRSLAFAVAAVFAYALLFELLGFTLATVVMAVPV
                     GMAFGGSWLQALAGGAGLGLLLYLGFDKLLDVVLPTGLLSFILGGR"
     gene            6647..8143
                     /locus_tag="PKN51_05620"
     CDS             6647..8143
                     /locus_tag="PKN51_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNT84613.1"
                     /translation="METLQYLMTGFGVALSTTNLLVAAIGALVGTIVGMLPGLGPING
                     VAILMPLAFALKLPAETALILLAAVYMGCEFGGRISSILLNVPGDAGAIMTALDGYPM
                     ARQGMGGVALSISAWSSFIGSLIATVGIVMFAPLLARWALAFGPAEYFALMCFAFACI
                     TGLMGDAPMKAALAAVIGLSLACVGLDSNSGVYRFTGGDVHLSDGIQFIVLVIGLFSI
                     SEILLMLEQHHSTAGIIKATGRSMFNLKEMAHTWWSTIRSSVVGFVVGVLPGAGATIA
                     SAITYTMEKRLAGPDNRFGDGDIRGVAAPEAANNASAAGSFVPMLTLGVPGSGTTAVM
                     MGALALYNITPGPALFTQNPTLVWGLIASFFVANVLLLIINIPLVGVFARMLQTPSWL
                     LVPAILAVSAVGVYSVHATTFDLVLMSVFGVVGYLLRKQGVPMAPLILGFVLGDMMEQ
                     NLRRALSITDGDVGILVESPISIGLWVLSGLMLIVPPLLRLRGRAKAR"
     gene            complement(8222..10489)
                     /gene="rnr"
                     /locus_tag="PKN51_05625"
     CDS             complement(8222..10489)
                     /gene="rnr"
                     /locus_tag="PKN51_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNT84614.1"
                     /translation="MLEEIEGTVQGHRDGHGFVLRDDGDADIYLPPNEMRAVLHRDRV
                     RVRIVRQDRRGRPEGRVLEIVERPPQPIIGRLLQEGGIWLVAPEDRRYGQDVLIPKTA
                     IGLARPGQVVVVELTEPPALYGQPVGRVKEVLGDIDDPGMEIEIAVRKYGVPHEFSDA
                     AIGEARALPDSVQPRDRAGRVDLTDVPLVTIDGEDARDFDDAVYCEPAVVRGRGKRPN
                     GWRLLVAIADVSHYVRTGAPLDVDAYDRATSVYFPRRVIPMLPEKLSNGLCSLNPEVE
                     RLSMVCDMLVTAQGEIHAYQFYPAVIRSHARLTYTEVAAILANTRGPEAARRQERVGD
                     LIHLHDVYRALLKARQQRGAVDFDTVETQIVCDEAGRIEKIVPRPRNDAHRLIEECML
                     AANVCSADFIGLGKHPGLYRVHEGPTPEKVEILRAYLKALGLGLSVSDEPRPEEFQRI
                     AAATAERADAEQIHMMLLRSMSQAIYTPINSGHFGLAYAAYTHFTSPIRRYPDLLVHR
                     VIKAILAERKYQLPTLPTPGEAHEKLSKRLKKDSLSRAQDRRDRPDRLSPEVQAWEAA
                     GLHCSANERRADEASRDVEAWLKCKYMKEHLGEEYAGTVSAATSFGLFVTLSDLHVEG
                     LVHITELGGEYFRFDEIRQELRGERTGIRYAIGTRVRVQVSRVDLDARKIDFRLVREG
                     EESLARVPRDKGAVAGAERGRPAKASSKRAARQQREAERGNARPSPMQALKSAVRKAA
                     AKKGAASGKPRKSRR"
     gene            10541..10625
                     /locus_tag="PKN51_05630"
     tRNA            10541..10625
                     /locus_tag="PKN51_05630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10575..10577,aa:Leu,seq:cag)
     gene            complement(10746..12164)
                     /gene="rimO"
                     /locus_tag="PKN51_05635"
     CDS             complement(10746..12164)
                     /gene="rimO"
                     /locus_tag="PKN51_05635"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNT84615.1"
                     /translation="MSETTTPPRIGFVSLGCPKALTDSELILTQLSAEGYQTSKTFEG
                     ADLVIVNTCGFIDEAVKESLDTIGEALTVNGKVIVTGCLGAKTIAAGADRADAGARNE
                     PENLIKHLHPSVLAVTGPHATQEVMDAVHRHLPKPHDPFLDLVPGAFGEAGLKLTPRH
                     YAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGEVLKEARALFEGGVKELLIISQDTSA
                     YGVDVKYRTGFWDGRPLRTRLLELVQALGELAAPHGAWVRLHYVYPYPHVDDILPLMA
                     EGKVLPYLDVPFQHSHPEVLRRMKRPASGEKNLERIARWRQICPELVIRSTFIAGFPG
                     ETEAEFQHLLDFMREARIDRAGCFAYSPVEGAAANQLPGMLAPELREERRARFMAVAE
                     AVSTAKLRERVGDTLQVLIDAAPALGRRGGLGRSYADAPEIDGMVHLLPPQKISKQLR
                     VGEFTRARVVDTQGHDLIAQPL"
     gene            complement(12203..12769)
                     /gene="phaR"
                     /locus_tag="PKN51_05640"
     CDS             complement(12203..12769)
                     /gene="phaR"
                     /locus_tag="PKN51_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNT84616.1"
                     /translation="MSPSKARTPAQPARDASAGAPQRVIKKYPNRRLYDTTTSSYITL
                     AQVRELVMAHQPFVVRDAKTGDDLTRSILLQIILEEEAGGAPMFTENMLANIIRFYGH
                     AMQGFMGSYLEKNIQAFMEIQSRLAEQSAGLSPELWAQFMRMQSPMMQGMMGSYLEQS
                     KNLFLQAQEQMSRQTEHMLGAFGIKRSG"
     gene            13200..13493
                     /locus_tag="PKN51_05645"
     CDS             13200..13493
                     /locus_tag="PKN51_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNT84617.1"
                     /translation="MWKLGRLLTLFRKELLLAWGLLRNPGAPLSSKLVTLLALLYVIS
                     PVDLVSDLIPVLGWLDDGLVAWLLWRLALRFAPAELVATLRGKLDARLRPAPR"
     gene            complement(13497..14468)
                     /locus_tag="PKN51_05650"
     CDS             complement(13497..14468)
                     /locus_tag="PKN51_05650"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNT84618.1"
                     /translation="MAKRTFLDFEQPIAELENKIEELRYVQSESAVDISEEIDQLDKK
                     SRQLTKDIYADLTPWQITKIARHPERPYTLDYVRECFTDFVELHGDRHYADDQSIVGG
                     LARFNGNACMVLGHQKGRDTKERAARNFGMTRPEGYRKALRLMKTAEKFRLPVFTFVD
                     TPGAFPGIDAEERGQSEAIGRNIYEMAQLEVPIITTIIGEGGSGGALAIAVADQVLML
                     QYAMYSVISPEGCASILWKTSDKAQDAAEALGITAHRLKALGMVDKIVSEPVGGAHRD
                     PRHMGTLLKRALGDAWRQVSDLKVKELLERRYERLMSYGRFNDTKER"
     gene            complement(14521..15171)
                     /locus_tag="PKN51_05655"
     CDS             complement(14521..15171)
                     /locus_tag="PKN51_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNT84619.1"
                     /translation="MAATKKIANQVATPGYWAEACAQLSKRDRVMKRLIPRFGEACLE
                     SRGDAFVTLARSIVGQQISVKAAQSVWNRFEALSRRVAPAQVLRLKVDDMRAAGLSAR
                     KVEYLVDLALHFDGGRIHVEQWQAMDDEAIIAELVAIRGIGRWTAEMFLMFHLLRPDV
                     LPLDDVGLIRGISVNYFSGEPVSRSEAREVAQAWAPWRSVATWYMWRSQDPVPVAY"
     gene            complement(15217..16593)
                     /gene="cysS"
                     /locus_tag="PKN51_05660"
     CDS             complement(15217..16593)
                     /gene="cysS"
                     /locus_tag="PKN51_05660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNT84620.1"
                     /translation="MTLRIHNTLTRALEDFSPIEPGHVRLYVCGITVYDLCHIGHARS
                     AVAFDVVQRWLKASGLRVTFVRNITDIEDKIIRRALENGETIRQLTDRMIAEMYRDFD
                     ALGIERPTHDPRATDYVPQMLDIIATLEGKGLAYRAESGDVNYAVRRFAGYGKLSGKT
                     LDELQAGERVAVSEGKHDPLDFVLWKSAKPSEPEGAKWDSPFGLGRPGWHIECSAMSC
                     ALLGNTFDIHGGGADLTFPHHENEIAQSEGANGQPLARLWMHNGFVNIDNEKMSKSLG
                     NFFTIREVLERFDAETIRFFIVRAHYRSPLNYSDVHLQDARAALKRLYTALDAVAPAE
                     VAIDWAHPHAARFKAAMDEDFGTPEAVAVLFDLASEVNRSRRPEDAGLLKALGACLGL
                     LQGDPKAFLRAGSALDEAAIAERILARAAAKQAKDFAEADRIRAALLAEGIVLKDSPA
                     GTTWEVAS"
     gene            16798..17352
                     /locus_tag="PKN51_05665"
     CDS             16798..17352
                     /locus_tag="PKN51_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT84621.1"
                     /translation="MTLRPFPFLPTARRALRPLLVVLALALGAAAAQADEYGEVQALQ
                     RAGQTSEALARADRHIAAHPNDPQMRFVKANLLSAGGRAGEAETLLVQLTRDYPELPE
                     PWNNLAVLYAGRGQLDQAQQALEAALRVNPAYATALENLGDVRLRQASEVYQRARQLD
                     PGSATRLTPRIDALRGLLGSGATR"
     gene            17446..18129
                     /locus_tag="PKN51_05670"
     CDS             17446..18129
                     /locus_tag="PKN51_05670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT84622.1"
                     /translation="MTLSRRTLARAAATLTAAAALGTALPAFAADPRVKFVTSEGDFV
                     VELYPDKAPKTVANFLQYVKDKHYDGTVFHRVIKGFMVQGGGFDRNYKEKETRASVIH
                     EGRKSLAAGLRNVTGTIAMARTNDPHSAKAQFFVNVVDNDFLDPVLIPSGDPVAKFEY
                     RGQTYTNVPRANLENHPQLFGYTVFGKVVSGMDTIEKIRNTPTGSGGPFPTDVPQKMV
                     VIESARQVN"
     gene            18163..18675
                     /locus_tag="PKN51_05675"
     CDS             18163..18675
                     /locus_tag="PKN51_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT84623.1"
                     /translation="MSNPQVELHIAGHGVITLELDQAKAPKSVANFLAYVRSGHYDNT
                     VFHRVIKNFMIQGGGFEPGMKQKPTQAPITNEADNGLKNARYSVAMARTSDPHSATAQ
                     FFINVVDNEFLNFKAPTAQAWGYAVFGQVVAGQDVVDAIKAVPTTRKGYHDDVPRDDV
                     VIEKAVVVGA"
     gene            18689..19468
                     /locus_tag="PKN51_05680"
     CDS             18689..19468
                     /locus_tag="PKN51_05680"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNT84624.1"
                     /translation="MTGRPALDELHASTDWRTVDVISDLHLRPEEPATFDAWARYLAA
                     PGADAVFILGDLFEVWVGDDAAQPGTFEARCGRVLAACPVPVFFLRGNRDFLVGSDFL
                     ARCGVRDLPADPTLLLWAGQRVLLSHGDLLCTDDTAYQQFRAQVRSADWQRAFATQPL
                     AQRQALARQMRAQSQAHQAGQTEYGHTDAALARRWLLDAGATLLIHGHTHRPADHALG
                     ADAQGRALTQVVLSDWHIDAGARRAQLLRLHADGQRQRIDL"
     gene            19480..20307
                     /locus_tag="PKN51_05685"
     CDS             19480..20307
                     /locus_tag="PKN51_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HNT84625.1"
                     /translation="MAALAALTRWLRRAPTPIPDALWHDTLARYPFLQRLSADQRQRL
                     QRLATHFIARKRFTGAHGLEVTDAMAVAVAAQACLPLLHLGPAERPEVALTWYDDFVT
                     IVLHPDDAVARRRVMDEAGVVHHYDEVLAGEAMQHGPVMLSWPAVQGAGMGPGSSVVI
                     HEFVHKMDMQAGGADGAPPLPRGFMGARTARQAAAHWHRTWAPAYERFREQVALAERF
                     GAAAPWLDAYAASAPAEFFAVACEAHFVQPERFAQDYPALSAALRAFFKQNNPPAQD"
     gene            20404..21876
                     /locus_tag="PKN51_05690"
     CDS             20404..21876
                     /locus_tag="PKN51_05690"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNT84626.1"
                     /translation="MTTPDTPLALLKDPSLLKTDALINGKWVKGKARFDVSDPATGRK
                     LADVADLGAREARSAIQAANAAWPAWRELTGKQRHAHLMAWFQLLMQHQDDLARLMTA
                     EQGKPLAEAKGEVAYGASFVEWFAEEAKRVQGETLTPFDRNKRLTVIQQPIGVCAAIT
                     PWNFPLAMITRKVAPALAAGCTVVIKPAEFTPLTALAAAELAQRAGIPAGVINVVTGL
                     DAPAIGRELCANDIVRHLSFTGSTEVGRILMAQCAPTVKKLALELGGNAPFIVFDDAD
                     LDSAVEGALASKYRNAGQTCVCANRLYVQDGVYDAFVKKLAARVKAMKVGNGFEDGVA
                     VGPLIEPAALAKVEQHVKDAVKKGGKVLTGGRRLSGQFYEPTVIAGATAAMRVAREET
                     FGPLAPVFRFRTEQEAIDAANATEFGLASYFYARDVGRITRVAEGLEYGMVGINTGLI
                     STEHVPFGGVKQSGLGREGSRHGMAEYLEMKYLCLGDVQK"
     gene            21902..23455
                     /locus_tag="PKN51_05695"
     CDS             21902..23455
                     /locus_tag="PKN51_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNT84627.1"
                     /translation="MLTRARLGAAAALLWLAPWAQAQPLWLDALGQPGPAAREAIGWL
                     TQAERDGLQPHDYDAARWARSIDPGQRLAATEAGAWDAALTQTLTRYLHELRHGRVPA
                     AALGARYDSVGQPAPDLAERLHDAVRNGRPAEALAAATPPWPQYPLLRTALAQYRALV
                     DDPAWRTALPPLPGDRLRPGQRWPGLAALAARLHALGDLDAAPATEPDTFDPALQHAV
                     KAFQERHGLSADGVLGKATLEALDVPPAARARQIALALERLRLTPLPAAERFVTVNVP
                     EFMLYAWQRQDQAVHQAFAMRVIVGKAVGTRTPLFDEDMRRIEFNPYWNIPPSIARGE
                     TLPKLRANPGYLAAQGMEFVGPGGQTSSAVTTELLDAVQAGQWRLRQRPGRLNALGDI
                     KFVLPNDQNIYLHHTPSVGLFQRARRDFSHGCIRIEQPVALAQWVLADDPDWSEARIR
                     QTMGQPRTVSVALPTPVPVLIVYRTVSVRDGRVHFAPDLYRQDALLERALAQRPHPAR
                     GPGPVRGAS"
     gene            23492..24061
                     /locus_tag="PKN51_05700"
     CDS             23492..24061
                     /locus_tag="PKN51_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNT84628.1"
                     /translation="MNNPHRRHFLGRSLGAAQLAVASVLLPAGGRALASAPDTLAPAR
                     RLAFNHLHTQERTALVYAVGEAFVPSALDHLNHFLRDHYSGDVGVIDPELFHLLHRVR
                     RELESEQPFHVISGYRSPHTNETLRTTRGGGVARRSLHMDGKAIDVRLPGVALADLRD
                     AALSLKAGGVGFYPRENFVHIDTGRVRSW"
     gene            complement(24081..>25372)
                     /locus_tag="PKN51_05705"
     CDS             complement(24081..>25372)
                     /locus_tag="PKN51_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNT84629.1"
                     /translation="GAGPAGLMAAERLSAAGVAVDVYDAMPSVGRKFLLAGKGGLNLT
                     HAEPLPDFIGRYGARAGAVAGWLHDFGPQALRDWAVGLGIDTFVGSSGRVFPTEMKAA
                     PLLRAWLQRLRHPQTPGAVPVRFHMRHRWVGWEGASDATETIASGACQKSAAGRFIFE
                     TPAGRRHLSPALALLALGGASWPRLGSDGAWVPWLAQVGAELAPLAPANCGFDAAGRA
                     GRGWTPFFAERFAGQPLKSVALTFTDGAGRHFARPGEFVVTASGVEGSLIYACGHLLR
                     DEIARAGQASFALDLLPGHTPAQVRAAVAHPRGSRSLSSHLSSRLGLAGVKMALLNEL
                     LTPEQLRDAAALAAAIKALPVTLTAPRPLPEAISSAGGVRLESLTEELELRCAPGLFC
                     AGEMLDWEAPTGGYLLTAAMASGVRAARGMLRRLDGR"
BASE COUNT         3636 a         9200 c         8729 g         3807 t
ORIGIN      
        1 gccgccgcgc cgaggctggc gggcagtgtg tgccgtccgg ggtccgctgc gcccgtagaa
       61 tccgcagcgc cttgcgtccc tgcccgtccc catctccatg aacatcgcca tcctcggcgc
      121 cggcatcgtc ggcgtgacca cggcctatga actggccgcc gatggccacc aggtgacggt
      181 gttcgagcgc cacgccagcg tcgccgaggc ggccagcttc gccaacgccg gggtggtggc
      241 accgggctac gtcacgccat gggcggcgcc gggcatgccg ctgaaggtgc tgcgctacct
      301 gctggcgcac cacgcgccgg tgcggctgcg ccggccactg gcgctggccg acatcgcctg
      361 gatgcgccac tggctggccg cctgcgaagg caccgcctac gcccgcaacc gagcgcgcat
      421 gcagcggctg gcgttctaca gccgccaccg gctgcaccgg ctggccgatg cgatggccta
      481 cgacttcgac cgcagcagtg gctacatggt gctgctgcgc gccgagcgcg accggcgcct
      541 ggcgcagccc gggctggccc tgctgcgcga gctgggcgtg ccgttcgacg agctcgacgc
      601 cgacgcggca cgccgcatcg agccggccct gcatccgcac accgcgctgg ccggcgccat
      661 ccacctgccg caggacgagg tgggcaattg ccgccagttc gccatcatcc tgcgtgacgc
      721 ggccgaacag ctgggcgcgc gctttgcctt caacaccacg gtgaccgcga tcgacaagac
      781 gcaaccagcg acgttccatg tggcgcacag cgccgtgccg ctacgcttcg acgccctcgt
      841 gctgtgcgcc ggcgtcggct cggccgaact gctgcggcca ctgggcctgc gcctgccgct
      901 gcgcccggtg tacggctatt cggtcagcgc cccggtgccc gaacccgcgc gggcgccgcg
      961 cagcggcgtg atggacgaac gctacaaggt cgccatcaca cgcatgggcc agcgcgtgcg
     1021 cgtggccggt tcggccgaaa tcggcggacg gccggacgac gtccaccacg ccgccttgca
     1081 gacgctgtac aaggtgctga ccgactggtt cccaggtgcc gcgcggctgt ccagcggcgt
     1141 gcaagtatgg aagggtgcgc gccccatgct gcccgacggt ccgccggtga tcggcgccag
     1201 tggcgtgcca ggtctgtggc tgaacctggg ccatggctcc agcggctggg cgctgtcctg
     1261 cggctcggcg cgcgtgctgg ccgaccagat cgccggccgc gtgcccgaca tcgacaccga
     1321 aggcctggac gtgcagcgct ggctgcgttg acggtggcgg gcggcaccgg agccacataa
     1381 tgccccatgc ccgacctgct gcccttcgat cgccccgccc cgctgttcga cctggccgcc
     1441 acccgacgca tcgaagccac ggcgcaagcc gcgctgccgc cgcataccct gatgcagcgc
     1501 gccggcctgg ccacggcacg gctggcgctg gcactggcgc cgcacgcgcg cacggtgtgg
     1561 gtcgcctgcg gcccgggcaa caacggcggc gacggcctgg aagccgccat gcacctgcag
     1621 ggctggggcc tgcacccggc ggtcacctgg ctgggcgacg aagcccatct gccggccgat
     1681 gcgcgcgcct cgctgcagcg cgcgcgcgcc gccggcgttg gcttcgccga tgcgccgcca
     1741 cccctgggac cgcacgacct ggcgatcgac gccctgctgg gcatcggcgc cacacgcgcg
     1801 ccgagcgggc gcatggccga ctggctgcgg caactgcgcc acggcccggc caccctgctg
     1861 gcgctcgacg tgcccagcgg gctggacgcc gacaccggct gcctgcatga ctcggatgct
     1921 actcattcga tagctgtcgg cgcttcatcc gcctgcgctg gaggccgatt tgatacacaa
     1981 ccgccgcacg gtgcgcggca cacgctcagc ctgctgacgc gcaagcccgg tctgttcacc
     2041 gcccagggcc gcgacgcctg cggcacgctg tggttcgacg atctcggcgt cgatccaggc
     2101 gaggtcgcgc ccaccgcctg gctgaacgcc ccgccggcgc tggcaccgcg tccgcacgac
     2161 agccacaagg gccgctttgg cgatgtggcg gtgatcggcg gcgccagcga caccgcgcag
     2221 ggcacggcca tgaccggcgc ggcgctgctg gcggcggcgg ccgcgctgca tgccggtgcc
     2281 ggccgggtgt acgtcggccc gctgggagcg acggcgccga ccttcgatcc ggtgcggccg
     2341 gagctgatgt tccgcccccc cgaccgcctg ccgctggcgc agctgaccgt ggtgtgcggc
     2401 tgcggcggcg gcgacgcggt gcgcgccgtg ctgcccgccg tgctggcgca ggcgccgagg
     2461 ctggtgctgg acgccgacgc gctgaacgcc atcgcgcagg acgcggcatt gcagcaggcg
     2521 ctggccgcgc gcgccgggcg tggtcagtcc agcgtgctca caccgcaccc gctggaggcg
     2581 gcgcgcctgc tgggcggcga cgtgcgtcag gtccaggccg accgcctgcg cgctgcgcag
     2641 gccctggccg ggcgcctgga atccaccgtg gtgctcaaag gctcgggcag cgtcatcgcc
     2701 gcgccgggcc agacgccgcg cctgaacgcc accggcaacg cccgcctggc cacgcccggc
     2761 tcgggcgacg tgctggccgg actgatcggc gcgcggctgg cgggcggccg gccggcgctg
     2821 gaggccgcct gcgacgccgt ccaccagcat ggcgcgctgg ccgacgcctg gcccgcgcac
     2881 accgccctca ccgccggcgc gctggcgcag cgcctgacgc cggcgtgacc gccgcccccc
     2941 cacccggaca ccgaccatgc gcatcctgct cgccgaagac gaacccgagc tggcaagctg
     3001 gctggcgcgt gcgctggagc gtggtggcct gctggccgac tgggtgagcg atggccgcga
     3061 cgtgctgccc agcctggcgg cacgccgcta cgacgcgctg gtgctcgatc tgggcctgcc
     3121 cgggctggac gggcaccagg tgctggccgc cctgcgcgcc accgaccagc gcctgccggt
     3181 gctgatcctg acggcacgcg actcgctgat cgagcgcgtc ggcacgctca accagggcgc
     3241 cgacgacttc ctggccaagc cgttcgacct ggcggagctg gaagcgcgcc tgcacgcgct
     3301 gatccgccgt gcgcgcggca gcgcccactg gcgccagagc tgcggcccac tggcctggga
     3361 cgccaccacc cgcggcttca ccctgaacga ggcgccgctg acgctgacgc cgcgcgagca
     3421 cgccgtgctg cgcgtgctga tccagcacgt gggcgagccg ctgtcgaagg cggaaatcct
     3481 cgaccgcgtg gtgtcggacg agcgcgacgt caaccccgag gcgatcgagg tgctggtgca
     3541 ccgcctgcgc aagcgcctgg cgggcagcca ggtgcgcatc ggcaccctgc gcgggctggg
     3601 ctacgtgctg gagcatggcg catgaggctc ggctggctgc gcgccagcct gcgccgagcg
     3661 ctggcgctgg tgctggcggc cggcatgttg ctggcctcgg gcgtgggcct gtggggcgcc
     3721 tggcgcagcg ccgccggcgc cgccaacgcc gcctacgacc gctcgctggc cggtgccatc
     3781 aaggccatcg acgccaacat caccaccgcc ggcggtggcc tgggggtgga gctgccctac
     3841 accctgctgg agttcttcca gctcaccgcc ggcggtcagg tgttcttccg tgtcgccacc
     3901 gaagacgggc tggtcgagat cggcaacacc gacctgccgc cgccggacac cggtctgatc
     3961 aacggccggc cgcgctttcg tgacgcgctc taccacggcc agccggtgcg gctgggctcg
     4021 tacacgcggg tgctcgatcc gcccccggcc ggtcacaccg ggcgaccgcg cgtggtgatc
     4081 caggtggccg agaccctgac gtcgcgccag gacttcacgc gcgcgctgct gctcgaagcg
     4141 gtggggcgca acgcgctgct ggtgacgctg gcgctgctgc tgatggcctg ggccaccggc
     4201 tgggcgctgc gcccgctggc gcgggtgtcg cgcgaggtgc aggcgcgccc gcccgacgac
     4261 ctgacgccgc tgccgctggc ggacgtgccg gccgaagcgc gtccgctggt ggaagccatg
     4321 aaccaccata tggcacgcca tcgacaggcc agcgaggcgc gccgacgctt cgtcgacgac
     4381 gcctcgcacc agctgcgcac gccgctgacg acgctgtcga cgcagctcgc ctacgcgctg
     4441 cgcgaagcgc cacccggccc cctgcgcgaa gccctgacgg caatgcgcgc gcagctcgac
     4501 ggtgccaccc ggcaggtcaa ccagatgctg gcgctggcgc gcgccgatgc ggccgagccg
     4561 cgcgccgaag tggtcgacct ggtggcgctg gcgcgcgagc tgacgcgcca tggctggccg
     4621 cgggcacgcg aggctggcat cgacctcggc ttcgaaagcc aagccgagca gctgccggcg
     4681 tcagtacagc ccatactgct gcaggaagcg ctgggcaacc tgctgcacaa cgcgctgcgc
     4741 tactgcccgg ccggcagccg cgtcacggtg cgggtggcgc aggagggcgg acaggcgcgc
     4801 ctcaccgtgc tggacgacgg ccccggcctg gcacccgatg aactggcgcg cgcgggcgag
     4861 cgcttctttc gcggccgcca ggcagcggcc ggcggcaccg gcctgggtct ggccattgcc
     4921 cgcgccgtgg ccgagcgcca tggcggcacc ctctcgctgc agaccggagc gggtggttgc
     4981 gggctggcgg tcagcctgct cctgcccctc cgggagttgc ccacatcccc gggttttccc
     5041 caggtggaga cggccccatt gaaagccgat tgaaagtcgg caggcgctag agtgacctgt
     5101 acggggcgcc gcgccgtcgg ctcccgaccc gatccgtcac cacgttcagg agactgcgcc
     5161 atgaaatttc gactccccct tgccgccctc accctggcct gcggccttgg cctggcccac
     5221 gccggcccgc tggacaagac cgaatgcatc gccccggcca agcccggcgg cggcttcgat
     5281 ctgacctgca agctggccca gtccagcctg ctcaacggca agtacatcag cgacccgatg
     5341 cgcgtgacct acatgccggg cggcatcggc gccgtggcct acaacgccgt ggtggcccag
     5401 cgcccggccg aaggcaacac catcgtcgcc ttctcgggcg gctcgctgct caacctggcg
     5461 cagggcaagt tcggccgcta cggcgaaaac gacgtgcgct ggctggccgc cgtcggcacc
     5521 gactacggcg ccgtcatcgt ggccgaggcg tcgcccttca agacgctcaa cgatctggtg
     5581 gccgccatca aggccgaccc gaccaaggtg gtgttcggcg ccggcggcac ggtgggcagc
     5641 caggactgga tgaaggccgc gctgaccgcc aaggccgccg gcctggaccc gcgcaaaatg
     5701 cgcttcgtgg ccttcgaggg cggcggcgaa gcctccaccg ccctgcaggg tggccacgtg
     5761 caggtgtact cgggcgacgc ctccgaggcc gaagaacaga tcaaggccgg cgccaagatc
     5821 cgcgtgctgg cggtgatggc cgacaagcgc ctggaaggcg gcctggccaa ggtgcccacc
     5881 gccaaggagc aaggactgga tgtggaatgg cccatcatcc gcggctacta catgggcccg
     5941 aaggtgagcg atgccgactt caaggtctgg accgacacct tcagcaagat gatggccacg
     6001 cccgaattcg ccaaggcacg cgccgagcgc agcctctacc cgttcgccaa gaccggcgcg
     6061 gacctggacg cctacgtcaa gcagcgcgtg gccgactacc gcaagctggc ggccgaattc
     6121 ggcctgaacg tcaccaaatg aggcggcgcg ccgcgccggg acgccggccg gcgcatccac
     6181 cggaggagca tcaaccatga gcgaccgcat tctgggcgtc gcctgcgtcg tggtggcggc
     6241 cggcatggcc tgggccgcca aggactacgc ggcgccgatt tcctacgagc cggttggccc
     6301 gcgtgcgttc ccgctgctgc tggccaccct gatggccgcc ggcggcctgt ggctgatcgt
     6361 gcggcccaac ctgcgcggca actggctgcg ccaggtgccg ctgcgctcgc tggcgtttgc
     6421 cgtcgccgcc gtgttcgcct acgcgctgct gttcgagctg ctgggcttca ccctggccac
     6481 ggtggtgatg gcggtaccgg tcggcatggc cttcggtggc agctggctgc aggcgctggc
     6541 cggtggcgcc ggcctgggcc tgctgctgta cctgggcttc gacaagctgc tggacgtggt
     6601 gctgcccacc gggctgctgt cgttcatcct cggggggcgc tgaaccatgg aaaccctgca
     6661 atacctgatg accggcttcg gcgtcgctct gtcgaccacc aacctgctgg tggccgccat
     6721 cggcgcgctg gtgggcacca tcgtcggcat gctgccaggg ttggggccga tcaacggcgt
     6781 ggccatcctg atgccgctgg cgttcgcgct caagctgccg gccgagacgg cgctgatcct
     6841 gctggccgcg gtctacatgg gctgcgagtt cggtggccgc atctcctcca tcctgctgaa
     6901 cgtgccgggc gacgccggcg ccatcatgac ggcgctggac ggctacccca tggcgcgcca
     6961 gggcatgggc ggcgtggcgc tgtcgatttc ggcctggagc tcgttcatcg gctcgctgat
     7021 cgccaccgtc ggcatcgtga tgttcgcgcc gctgctggcg cgctgggcac tggccttcgg
     7081 cccggccgag tacttcgccc tgatgtgctt cgccttcgcc tgcatcaccg gcctgatggg
     7141 cgacgccccg atgaaggcgg cgctggcggc ggtgatcggc ctgtcgctgg cctgcgtcgg
     7201 gctggactcc aactccggcg tctaccgctt caccggcggc gacgtgcacc tgtcggacgg
     7261 catccagttc atcgttctgg tgatcggcct gttctccatc agcgaaatcc tgctgatgct
     7321 ggagcagcac cacagcacgg ccggcatcat caaggccacc gggcgctcga tgttcaacct
     7381 caaggaaatg gcccacacct ggtggtccac catccgctcg tcggtggtgg ggttcgtggt
     7441 cggcgtgctg ccgggcgccg gcgccaccat cgccagcgcc atcacctaca ccatggaaaa
     7501 gcgcctggcc gggccggaca accgcttcgg cgacggcgac atccgcggtg tggcggcccc
     7561 ggaggcggcc aacaatgcct cggcggcggg ctcgttcgtg cccatgctga cgctgggcgt
     7621 gcccggctcg ggcactacgg cggtgatgat gggggcgctc gcgctctaca acatcacgcc
     7681 cggcccggcg ctgttcacgc agaacccgac cctggtgtgg ggcctgatcg cctcgttctt
     7741 cgtcgccaac gtgctgctgc tgatcatcaa catcccgctg gtcggcgtgt tcgcgcgcat
     7801 gctgcagacg cccagctggc tgctggtgcc cgccatcctg gcggtcagcg cggtgggggt
     7861 gtactcggtg cacgccacca ccttcgatct ggtgctgatg tcggtgttcg gcgtggtcgg
     7921 ctacctgctg cgcaagcagg gcgtgccgat ggcaccgctg atcctgggtt tcgtgctggg
     7981 cgacatgatg gagcagaacc tgcgccgcgc actgtcgatc accgacggcg acgtcggcat
     8041 cctggtggaa agcccgattt ccatcggtct gtgggtgctc agcggtctga tgctgatcgt
     8101 gccgccgctg ttgcgcctgc gggggcgcgc caaggcccgc tgaggccttg aacagcagca
     8161 aaactgcttc cgtcgcccgc ccagtatgcg caagccgctc tcattttgag agcggttttt
     8221 ttcaacgccg gctcttgcgg ggtttgccgc tggcggcgcc cttcttggcc gccgccttgc
     8281 gcacggccga cttcagcgcc tgcatgggtg agggccgcgc gttgccgcgt tcggcctcgc
     8341 gctgctggcg cgcggcgcgc ttgctcgagg ccttggctgg ccggccgcgc tcggcgcctg
     8401 ccaccgcgcc cttgtcgcgg gggacgcgcg ccagactttc ctcgccttca cgcaccagcc
     8461 ggaagtcgat cttgcgcgcg tccagatcga cccggctgac ctgcacccgc acccgggtgc
     8521 cgatggcgta acggatgccg gtgcgctcgc cgcgcagttc ctggcggatc tcgtcgaagc
     8581 ggaagtattc cccgcccagc tcggtgatgt gcaccaggcc ttcgacgtgc aggtcgctga
     8641 gggtgacgaa caggccgaag ctggtggctg cgctgacggt gccggcgtat tcctcgccca
     8701 ggtgctcctt catgtacttg cacttgagcc aggcctcgac gtcgcggctg gcttcgtcgg
     8761 cacggcgctc gttggcgctg caatgcaggc ccgccgcctc ccacgcctgc acctcgggcg
     8821 acaggcgatc cggcctgtca cgccggtcct gcgcgcgcga caggctgtcc ttcttcaggc
     8881 gcttgctcag cttttcatgg gcttcgccgg gcgtgggcag ggtcggcaac tggtacttgc
     8941 gctcggccag gatggccttg atgacgcggt gcaccagcag atcggggtag cgccggatcg
     9001 ggctggtgaa atgcgtgtag gcggcatatg ccagtccgaa atgcccgctg ttgatgggcg
     9061 tgtagatggc ctggctcatg gaacgcagca gcatcatgtg gatctgctcg gcgtcggcgc
     9121 gctcggcggt ggcggcggcg atgcgctgga attcctcggg ccgcggctcg tcgctgaccg
     9181 acaggcccag gcccagcgcc ttcaggtagg cgcgcaggat ctcgaccttc tccggcgtcg
     9241 gcccttcgtg cacgcggtac agaccggggt gcttgcccag gccgatgaag tcggcgctgc
     9301 acacgttggc ggccagcatg cattcctcga tcaggcggtg cgcgtcgttg cgcgggcgcg
     9361 gcacgatctt ttcgatgcgg ccggcctcgt cgcagacgat ctgcgtctcc acggtatcga
     9421 agtccaccgc gccgcgctgc tggcgcgcct tcagcaaggc gcggtacacg tcgtgcaggt
     9481 gaatcaggtc gcccacgcgc tcctgccgcc gcgccgcctc gggcccgcgg gtgttggcca
     9541 ggatggccgc cacctcggtg taggtcaggc gtgcatggct gcgaatcacc gcggggtaga
     9601 actggtaggc gtggatctcg ccctgcgccg tcaccagcat gtcgcacacc atgctcaggc
     9661 gctccacctc ggggttgagc gagcacagac cgttgctgag cttttccggc agcatgggaa
     9721 tgacgcgacg cggaaagtac acgctggtgg cgcggtcgta ggcgtccacg tcgagcggcg
     9781 cgccggtgcg cacgtagtgg ctgacgtcgg cgatcgccac cagcaggcgc cagccgttgg
     9841 gccgcttgcc gcgcccgcgc accaccgccg gctcgcagta gacggcgtcg tcgaaatcac
     9901 gcgcgtcttc gccgtcgatg gtgaccagcg gcacatccgt caggtccacg cggccagcgc
     9961 ggtcgcgcgg ctgcacgctg tcgggcaggg cgcgcgcctc gccgatggcg gcgtcggaga
    10021 actcgtgcgg cacgccgtac ttgcgcaccg cgatttcgat ctccatgccg gggtcatcga
    10081 tgtcgcccag cacttccttg acgcgcccga ccggctggcc atagagcgcg ggcggctcgg
    10141 tcagctcgac caccaccacc tgacccggcc gggccagacc gatggcggtc ttgggaatca
    10201 gcacgtcctg gccatagcgc cggtcttccg gggccaccag ccagatgccg ccttcctgca
    10261 gcaggcgacc gatgatgggt tgcggcgggc gctcgacgat ttcgagcacg cgcccctcgg
    10321 ggcgcccgcg ccggtcctgc cgcacgatgc gcacgcgcac gcggtcgcgg tggagcaccg
    10381 cgcgcatctc gttggggggc agatagatgt cggcgtcgcc gtcatcgcgc agcacgaaac
    10441 cgtgcccgtc gcggtggcct tgcaccgtgc cttcaatttc ttccagcagg ctgctggtgg
    10501 agtcgagatt tttgatatag aatcctagct ttcctgaaat gcccaggtgg cggaattggt
    10561 agacgcacta gtttcaggta ctagcgctga gaggcgtgga ggttcgagtc ctctcctggg
    10621 caccaaaaca aaagaagatt acagccgcca acaccgctgc aatcgatgac aagccaccca
    10681 tcgggtggct tttttcttgc ccgcccgagc cagctgttgc gcgtacgcat cagttttcat
    10741 ccggctcaga ggggctgggc gatcaggtcg tggccctggg tgtcgaccac ccgcgcccgc
    10801 gtgaactcgc ccacccgcag ctgcttgctg attttttgcg gcggcagcaa atgcaccatg
    10861 ccgtcgatct cgggcgcgtc ggcgtagctg cgccccagcc cgccgcgccg ccccagggcc
    10921 ggcgcggcat cgatcagcac ctgcagggtg tcgcccacgc gttcgcgcag cttggcggtg
    10981 gacacggctt cggccaccgc catgaagcgc gcgcggcgct cttcgcgcag ctcgggcgcc
    11041 agcatgccgg gcagctggtt ggcggcggcg ccttcgaccg gtgagtaggc aaagcagccg
    11101 gcgcggtcga tgcgcgcctc gcgcatgaag tccagcaggt gctggaactc ggcttcggtc
    11161 tcgcccggaa agccggcgat gaaggtggag cggatcacca gttcgggaca gatctgacgc
    11221 cagcgcgcga tgcgctccag gttcttctcg ccgctggccg ggcgcttcat gcggcgcagc
    11281 acctcggggt ggctgtgctg gaacggcacg tccaggtacg gcagcacctt gccttcggcc
    11341 atcagcggca ggatatcgtc cacatggggg tacgggtaca cgtaatgcaa gcgcacccag
    11401 gcgccatgcg gcgcggccag ctcgcccaac gcctgcacca gctccagcag gcgcgtgcgc
    11461 agcgggcggc cgtcccagaa gccggtgcgg tacttcacat ccacgccgta ggccgaggtg
    11521 tcctggctga tgatcagcag ctccttgacg ccaccttcga acagcgcgcg cgcttccttc
    11581 agcacctcgc cgatggggcg gctcaccagg tcgccgcgca tcgaggggat gatgcagaag
    11641 gtgcagcggt ggttgcagcc ctcgctgatc ttcaggtagg cgtagtggcg tggcgtgagc
    11701 ttcaggccgg cttcgccgaa ggcgccgggc accaggtcga ggaaaggatc gtgcggcttg
    11761 ggcaggtgcc ggtgtacggc gtccatcacc tcctgggtgg cgtgcgggcc ggtcacggcc
    11821 agcaccgagg ggtgcaggtg cttgatcagg ttttcaggct cattccgggc cccagcgtcc
    11881 gcccgatcag cgccagcagc tatggttttt gcaccaaggc agccggtgac gatgaccttg
    11941 ccgttgaccg tcagggcctc gccgatggtg tccaggcttt ccttgaccgc ctcgtcgatg
    12001 aacccgcagg tgttgacgat gaccagatcg gcaccttcga aggtcttgct ggtctggtag
    12061 ccctcggccg acagctgggt caggatcagc tcggagtcgg tcagcgcctt ggggcagccc
    12121 agggacacga agccgatgcg cggcggcgtg gtggtttcac tcatgggggc gattgtcgca
    12181 gccccgcgcg ccgaccgtac cgtcaaccgc tgcgcttgat gccgaaggca cccagcatgt
    12241 gctcggtctg ccggctcatc tgctcctgcg cctgcaggaa caggttcttg gactgctcca
    12301 ggtagctgcc catcatgccc tgcatcatgg gcgactgcat gcgcatgaac tgcgcccaca
    12361 actccgggct gaggccggcg gactgctcgg ccagccggga ctgaatctcc atgaaggcct
    12421 ggatgttctt ttccaggtag ctgcccatga agccctgcat ggcatgaccg tagaagcgga
    12481 tgatgttggc cagcatgttc tcggtgaaca tgggggcgcc gccggcttct tcttccagga
    12541 tgatctgcag caggatgctg cgcgtcaggt cgtcgcccgt cttggcgtcg cgcaccacga
    12601 acggctggtg cgccatcacc agctcgcgca cctgggccag cgtgatgtag gacgaggtgg
    12661 tggtgtcgta gagccgccgg ttggggtatt tcttgatcac ccgctgcggc gcgccggccg
    12721 acgcgtcgcg cgccggctgg gcgggagtcc tggctttgct ggggctcatg ggttcacggg
    12781 tcgctgtaac tgctgcactg caacatattg taggggcggc ggcgccaccc tgacccaacc
    12841 accgacccat ggctttcccg cacaccgacg ctgcgcagcg ctcgccctgc gggaagccag
    12901 aaaaaggctt gcggcggcgt tgcgcctctt gccaaggccg ccagcatggg ctgcgagccg
    12961 cgccttgccg caagcctttt tctggcttcc atagcaacgc cgcatggcgc ggcgagcgcg
    13021 gtgcgggtgg gtgcgcagcg tgctcacccg ggaggtgaag gccatcgcag ccgccagacc
    13081 ccgcatcgat gcggtctgcc cgaagtagac cagcggtcaa cggccgcttc caggttcaac
    13141 cagcgcggcg gcgcacaccc tgctaccatg cgcgccttcg cccgaaggga gtcgtcacca
    13201 tgtggaagct cggtcgcctg ctcacgctgt ttcgcaagga gttgttgctc gcctggggct
    13261 tgctgcgcaa cccgggcgcg ccgctgtcgt ccaagctggt gacgctgctg gcactgctgt
    13321 acgtgatcag cccggtcgat ctggtgtccg acctgattcc ggtgctgggc tggctggacg
    13381 atggcctggt ggcctggctg ctgtggcggc tggcgctgcg ctttgcgccg gccgagctgg
    13441 tggccacgct gcgcggcaag ctcgacgccc ggctgcgccc ggcgccgcgc tgaggctcag
    13501 cgctccttgg tgtcgttgaa gcgcccgtag ctcatcaggc gctcgtagcg gcgctccagc
    13561 agttccttga ccttcaggtc gctgacctgg cgccaggcgt cgcccagcgc acgcttgagc
    13621 agcgtgccca tgtggcgcgg gtcgcggtgc gcgccgccga ccggctcgct gacgatcttg
    13681 tcgaccatgc ccagcgcctt cagccggtgc gcggtgatgc ccagcgcttc ggcggcgtcc
    13741 tgcgccttgt cgctggtctt ccacaggatg gaggcacagc cttcgggact gatgaccgaa
    13801 tacatggcgt actgcagcat cagcacctga tcggccacgg cgatggccag cgcgccgccg
    13861 gagccgcctt caccgatgat ggtggtgatg atcggcacct cgagctgcgc catttcgtag
    13921 atgttgcgac cgatggcctc ggactggccg cgctcttcgg cgtcgatgcc gggaaaggcg
    13981 ccgggcgtgt cgacgaaggt gaacaccggc agcctgaatt tttcggccgt cttcatcagc
    14041 cgcagcgcct tgcggtagcc ctcgggccgg gtcatgccga agttgcgcgc ggcgcgctcc
    14101 ttggtgtcgc gccccttctg gtgacccagc accatgcagg cgttgccgtt gaagcgcgcc
    14161 aggccgccga cgatgctctg gtcgtcggcg tagtggcggt cgccgtgcag ttcgacgaaa
    14221 tcggtgaagc actcgcgcac gtagtccagc gtgtacggcc gctcggggtg acgcgcgatc
    14281 ttggtgatct gccagggcgt caggtcggca tagatgtcct tggtgagctg gcggctcttc
    14341 ttgtcgagct ggtcgatttc ttccgagatg tcgaccgcgc tctcgctctg cacgtagcgc
    14401 agctcttcga tcttgttttc aagctccgcg atgggctgct cgaaatcaag aaatgtccgt
    14461 ttggccaagt tcggctcctt ggtgtcgcaa cagctgccga tgttaccgga tgcgatcgcc
    14521 tcagtaggcg accggcaccg gatcctgcga gcgccacatg taccaggtgg ccacgctgcg
    14581 ccagggcgcc caggcctgcg ccacctcgcg cgcttcgctg cggctgaccg gctcgccgga
    14641 gaagtagttg acgctgatgc cgcggatcag accaacgtcg tccagcggca gcacatcggg
    14701 gcgcagcaga tggaacatca ggaacatctc ggcggtccag cggccgatgc cgcggatggc
    14761 caccagctcg gcgatgatgg cctcgtcgtc catcgcctgc cattgctcga catggatgcg
    14821 gccgccgtcg aaatgcagcg ccagatccac caggtactcg accttgcgcg ccgacaggcc
    14881 ggcggcgcgc atgtcgtcca ccttcagcct gagcacctgg gccggcgcca cgcggcgcga
    14941 cagggcctcg aaacggttcc acaccgactg cgccgccttg accgaaatct gctggccgac
    15001 gatggagcgc gccagggtga cgaaggcatc gccacgcgac tccaggcagg cctcgccaaa
    15061 gcgcgggatg agccgcttca tgacgcggtc gcgcttgctg agctgcgcgc aggcctcggc
    15121 ccagtagccc ggcgtggcca cttggtttgc tatttttttt gtagccgcca ccgcactacc
    15181 cgcctgcgct ggaggtcaga aacattctga acccgatcac gacgcaacct cccaggtggt
    15241 gccggccggc gagtccttga gcacgatgcc ttcggccagc agggcggcac ggatgcggtc
    15301 ggcctcggca aagtccttcg cctgcttggc ggccgcgcgg gccaggatgc gctcggcaat
    15361 ggcggcttcg tccagcgcgc tgccagcccg caggaaggcc ttcggatcgc cctgcagcag
    15421 acccaggcag gcgcccaggg ccttgagcag gccggcgtcc tcgggccggc ggctgcggtt
    15481 gacctcgctg gccagatcga acagcacggc caccgcttcg ggcgtgccga agtcctcgtc
    15541 catggccgcc ttgaagcgcg cggcgtgggg gtgggcccag tcgatcgcca cctcggcggg
    15601 cgccaccgca tccagcgcgg tgtacaggcg cttgagggcc gcgcgcgcat cctgcaggtg
    15661 cacgtcgctg tagttgagcg ggctgcggta gtgggcgcgc acgatgaaga agcggatggt
    15721 ctcggcgtcg aaccgttcca gcacctcgcg aatggtgaag aagttgccca gcgacttgga
    15781 catcttctcg ttgtcgatgt tgacgaagcc gttgtgcatc cacaggcggg ccagtggctg
    15841 gccgttcgca ccttcgcttt gcgcgatctc gttttcgtgg tgcgggaacg tgaggtcggc
    15901 gccgccgccg tggatgtcga aggtgttgcc cagcagcgcg cagctcatgg ccgagcactc
    15961 gatgtgccag ccggggcggc ccaggccgaa cgggctgtcc cacttggcgc cctcgggctc
    16021 gctgggcttg gcggacttcc acagcacgaa gtccagcggg tcgtgcttgc cctcgctgac
    16081 ggccacgcgc tcgccggcct gcagctcgtc gagcgtcttg cccgacagct tgccgtaacc
    16141 ggcgaaccgg cgcaccgcgt agttgacgtc gccgctctcg gcccgatagg ccaggccctt
    16201 gccttccagc gtggcgatga tgtccagcat ctgcggcacg tagtcggtgg cgcgcgggtc
    16261 gtgcgtgggc cgctcgatgc ccagggcgtc gaagtcgcgg tacatctcgg cgatcatgcg
    16321 gtcggtcagc tggcggatgg tttcgccgtt ctccagcgcg cggcggatga tcttgtcctc
    16381 gatgtcggtg atgttgcgca cgaaggtcac gcgcaggccg ctggccttga gccagcgctg
    16441 caccacgtcg aaggccacgg ccgagcgcgc atgaccgatg tggcacaggt catacaccgt
    16501 gatgccgcac acgtacaggc gcacgtggcc gggttcgatg ggagaaaagt cctccagggc
    16561 acgcgtcagc gtgttgtgga tgcgcaaggt catggaaaaa cgagcgggcg aaagcgggcg
    16621 gcagcaggtc acatgagccg cagacgccgg tgggcgctgc cgctgcaccc tccgcgtggc
    16681 ggcggcccgg ctggcctgct tggctacaat tcattcagta taagacgcga cccgcgtgcg
    16741 ggccagcacg cggcgccccg accgcccagg ctgcgccgcc cggcggcccg attgcccatg
    16801 acgctgcgcc ccttcccctt tctgccgacc gcccgccgcg cgctgcgccc gctgctggtg
    16861 gtgctcgcgc tggcgctagg cgccgcggcc gcgcaggccg acgaatatgg cgaggtgcag
    16921 gcgctgcagc gcgccggcca gaccagcgaa gcgctggcgc gcgccgaccg ccacatcgcc
    16981 gcccacccca acgatccgca aatgcgcttc gtcaaggcca acctgctgag cgccggcggc
    17041 cgcgccggcg aggccgagac cctgctggtg cagctgacac gcgactaccc cgaactgccc
    17101 gaaccctgga acaacctggc cgtgctgtac gccggccgcg gccagctgga ccaggcgcag
    17161 caggccctcg aggccgcgct gcgcgtcaac ccggcctacg ccaccgcgct ggaaaacctg
    17221 ggcgacgtgc gcctgcgcca ggccagcgag gtctaccagc gcgcacgcca gctcgacccg
    17281 ggcagcgcca cgcgcctgac accccgcatc gacgccctgc gcggcctgct gggcagcggc
    17341 gccacccgtt agtcgctggc ggcgggcgat gcggcggaac tcgcgcaccg ctgccggcac
    17401 caagctgttt ttttctgctg ttcctgaacc tcggagtccg aacccatgac gctctcccgc
    17461 cgaaccctcg cccgtgccgc cgccacgctg accgcggccg ccgccctggg caccgccctg
    17521 cccgccttcg ccgccgaccc gcgcgtgaag ttcgtcacca gcgagggcga tttcgtggtc
    17581 gagctgtacc ccgacaaggc ccccaagacg gtcgccaact tcctgcagta cgtgaaggac
    17641 aagcactacg acggcaccgt cttccaccgc gtcatcaagg gcttcatggt gcagggcggc
    17701 ggctttgaca gaaactacaa ggaaaaggaa acccgcgcct cggtgatcca cgaaggccgc
    17761 aagtcgctcg ccgccggcct caggaacgtc accggcacca tcgccatggc gcgcaccaac
    17821 gacccgcact cggccaaggc gcaatttttc gtcaacgtgg tcgacaacga ttttctcgac
    17881 ccggtgctga tcccctccgg cgacccggtg gccaagttcg agtaccgcgg ccagacctac
    17941 accaacgtgc cgcgcgccaa cctcgaaaac caccctcagc tgttcggcta caccgtgttc
    18001 ggcaaggtcg tcagtggcat ggacacgatc gaaaagatcc gcaacacgcc caccggcagc
    18061 ggcggcccct tccccaccga cgtgccccag aagatggtgg tgatcgaatc ggcccggcag
    18121 gtcaactgaa cattcctcac cctcaccacg aaagcccctc tcatgagcaa cccccaagtc
    18181 gaactgcaca tcgccggcca cggcgtcatc acgctggagc tggaccaggc caaggcgccc
    18241 aagtcggtgg ccaacttcct ggcctacgtc cgcagcggcc actacgacaa cacggtgttt
    18301 caccgcgtca tcaagaactt catgatccag ggcggcggct tcgagcccgg catgaagcaa
    18361 aagcccaccc aggcgcccat caccaacgaa gccgacaacg gcctgaagaa cgcccgctac
    18421 agcgtggcca tggcgcgcac cagcgacccg cactcggcca cggcgcagtt cttcatcaac
    18481 gtggtggaca acgaattcct gaacttcaag gcacccaccg cccaggcctg gggctacgcc
    18541 gtgttcggcc aggtggtggc gggccaggac gtggtcgacg ccatcaaggc cgtgcccacc
    18601 acccgcaagg gctaccacga cgatgtcccg cgcgacgacg tggtgatcga aaaggccgtg
    18661 gtggtcggcg cctgacaacc cgcgcccgat gaccggccgc ccggcgctgg acgagctgca
    18721 cgccagcacc gactggcgca cggtcgatgt catctccgac ctgcacctca ggcccgaaga
    18781 gcccgccacc ttcgacgcct gggcgcgcta tctggccgcg ccgggcgccg atgcggtgtt
    18841 cattctgggc gatctgttcg aggtctgggt gggtgacgac gcggcgcagc ccggcacctt
    18901 cgaagcgcgc tgcgggcgag tgctggcggc ctgcccggtg cccgtgttct tcttgcgcgg
    18961 caaccgcgac tttctggtcg gcagcgattt tctggcgcgc tgcggcgtgc gcgacctgcc
    19021 ggccgacccg acgctgctgc tgtgggccgg ccagcgcgtg ctgctcagcc acggcgacct
    19081 gctgtgcacc gacgacaccg cctaccagca gtttcgcgcc caggtgcgca gcgccgactg
    19141 gcagcgcgcc tttgcgacgc agccactggc gcagcgccag gcgctggcgc ggcagatgcg
    19201 ggcgcaaagc caggcccacc aggcgggcca gaccgaatac ggccacaccg atgccgcgct
    19261 ggcgcgccgc tggctgctgg acgccggcgc cacgctgctg atccacggcc acacccaccg
    19321 gccggccgac cacgcgctgg gcgccgacgc gcagggccgc gccctgaccc aggtggtgct
    19381 gagcgactgg cacatcgacg ccggcgcgcg ccgcgcccag ctgctgcgcc tgcacgccga
    19441 cgggcagcgc cagcgcatcg acctgtgatc tgagcaccca tggcagcgct ggccgccctg
    19501 acccgctggc tgcgccgcgc gcccacgccg atcccggacg cgctgtggca cgacaccctg
    19561 gcgcgctacc cgttcctgca gcggctgagc gccgatcagc gccagcgcct gcagcgcctg
    19621 gccacgcact tcatcgcccg caagcgcttc accggtgccc acgggctgga agtcaccgac
    19681 gccatggcgg tggcggtggc ggcgcaggcc tgcctgccgc tgctgcacct ggggcccgcc
    19741 gagcggcccg aggtggcgct gacgtggtac gacgatttcg tcaccatcgt gctgcacccc
    19801 gacgacgcgg tggcgcgccg gcgcgtgatg gacgaggccg gcgtggtgca ccactacgac
    19861 gaggtgctgg ccggcgaggc catgcagcac ggcccggtga tgctcagctg gcccgccgta
    19921 cagggcgccg gcatgggccc cggcagcagc gtggtgatcc acgagttcgt gcacaagatg
    19981 gacatgcagg ccggcggcgc cgacggcgcg ccgccgctgc cgcgcggctt catgggcgcg
    20041 cgcacggcac gccaggccgc cgcccactgg catcgcacct gggcgccggc gtacgagcgg
    20101 tttcgcgagc aggtcgcgct ggccgagcgc ttcggcgctg ccgcgccctg gctcgacgcc
    20161 tacgccgcca gtgcgccggc cgagttcttc gccgtcgcct gcgaagcgca cttcgtgcag
    20221 cccgagcgct ttgcccagga ctacccggcc ctgagcgccg ccctgcgtgc gttcttcaag
    20281 caaaacaacc cgccggcgca ggactgacat gcgccatcag ctattattac aatagcattc
    20341 gagtaaccct gacggccggc aagcgctgcc tacaatcgtg cgatttcgca ggagacgacc
    20401 cccatgacca cccccgacac cccgctggcc ctgctcaagg accccagcct gctcaagacc
    20461 gacgccctga tcaacggcaa atgggtcaag ggcaaggcgc gtttcgacgt cagcgacccg
    20521 gccaccggcc gcaagctggc cgacgtggcc gacctgggcg cgcgcgaagc ccgcagcgcc
    20581 atccaggccg ccaatgccgc ctggccggcc tggcgcgagc tgaccggcaa gcagcgccac
    20641 gcccacctga tggcctggtt ccagctgctg atgcagcacc aggacgacct ggcgcgcctg
    20701 atgaccgccg aacaaggcaa accgctggcc gaagccaagg gcgaagtcgc ctatggcgcc
    20761 agcttcgtcg aatggttcgc cgaagaagcc aagcgcgtgc agggcgaaac cctgaccccg
    20821 ttcgaccgca acaagcgcct gaccgtgatc cagcagccga tcggcgtgtg cgccgccatc
    20881 acgccgtgga acttcccgct cgccatgatc acgcgcaagg tggcgccggc gctggcggcc
    20941 ggctgcacgg tggtcatcaa gccggccgaa ttcaccccgc tgaccgcgct ggccgcggcc
    21001 gagctggccc agcgcgccgg catcccggcc ggcgtcatca acgtcgtcac cgggctggat
    21061 gcgccggcca tcggtcgcga gctgtgcgcc aacgacatcg tgcgccacct gtcgttcacc
    21121 ggctcgaccg aggtcggccg catcctgatg gcgcaatgcg cgcccaccgt caagaagctg
    21181 gcgctggagc tgggcggcaa cgcgcccttc atcgtgttcg acgacgccga cctcgactcg
    21241 gcggtcgaag gcgcgctggc cagcaaatac cgcaacgccg gccagacctg cgtctgcgcc
    21301 aaccgcctgt acgtgcagga cggcgtgtac gacgccttcg tcaagaagct ggccgcccgc
    21361 gtcaaggcca tgaaggtggg caacgggttc gaggacggcg tcgccgtcgg cccgctgatc
    21421 gagccggccg ccctggccaa ggtggagcag cacgtcaagg acgcggtcaa gaaaggcggc
    21481 aaggtgctga ccggcggccg gcgcctgtcg ggccagttct acgagcccac cgtgatcgcc
    21541 ggcgccaccg ccgccatgcg ggtggcgcgc gaagaaacct tcggcccgct ggcgccggtg
    21601 ttccgcttcc gcaccgagca ggaagccatc gacgctgcca acgccaccga attcggcctg
    21661 gccagctact tctacgcccg cgacgtcggc cgcatcacgc gcgtggccga ggggctggaa
    21721 tacggcatgg tgggcatcaa caccggcctg atctcgaccg agcatgtgcc cttcggcggc
    21781 gtcaagcaat ccggcctggg gcgcgaaggc tcgcgccacg gcatggccga gtacctggag
    21841 atgaagtacc tgtgcctggg cgacgtgcag aagtgaaccc ggcgccgcgc ccggcctggc
    21901 gatgctgacg cgcgcccgtc tcggcgccgc cgccgccctg ctgtggctgg cgccctgggc
    21961 gcaggcgcag ccgctgtggc tggacgcgct gggccagccc ggccccgccg cgcgcgaggc
    22021 gatcggctgg ctgacgcagg ccgagcgcga cggcctgcag ccgcacgact acgacgccgc
    22081 gcgctgggcg cgcagcatcg accccggcca gcggctggcg gcgaccgagg ccggcgcctg
    22141 ggacgcggcc ctgacgcaga ccctgacgcg ctacctgcac gaactgcgcc acggccgcgt
    22201 gcccgccgcc gccctgggcg cccgctacga cagcgtcggt cagcccgcgc ccgacctggc
    22261 cgagcgcctg cacgacgcgg tgcgcaacgg ccggccggcc gaggccctgg ccgccgccac
    22321 cccgccctgg cctcagtacc cgctgctgcg caccgccctg gcgcagtacc gcgcgctggt
    22381 ggacgacccg gcctggcgca ccgcgctgcc gcccctgccc ggcgaccgct tgcgccccgg
    22441 ccagcgctgg cccgggctgg cggcgctggc ggcgcgcctg cacgcgctgg gcgatctgga
    22501 cgccgccccg gccaccgaac cggacacgtt cgaccccgcg ctgcagcacg ccgtcaaggc
    22561 cttccaggag cgccacggcc tgagcgccga cggcgtgctg ggcaaggcga cgctggaggc
    22621 gctggacgtg ccacccgccg cccgcgcacg ccagatcgcg ctggcgctgg agcgcctgcg
    22681 cctgaccccg ctgccggcgg ccgagcgctt cgtcaccgtc aacgtgcccg aattcatgct
    22741 gtacgcctgg caacggcagg accaggccgt gcaccaggcc ttcgccatgc gggtgatcgt
    22801 cggcaaggcg gtcggcacgc gcaccccgct gttcgacgag gacatgcgcc gcatcgagtt
    22861 caacccgtac tggaacatcc cgccctccat cgcgcgcggc gaaaccctgc ccaagctgcg
    22921 cgccaacccc ggctacctgg cggcgcaggg catggagttc gtcggccccg gcggccagac
    22981 cagcagcgcc gtcaccaccg agctgctgga cgcggtgcag gccggccagt ggcgcctgcg
    23041 ccagcgcccg ggccggctga acgcgctggg cgacatcaag ttcgtgctgc ccaacgacca
    23101 gaacatctac ctgcaccaca cgccctcggt cggcctgttc cagcgcgccc ggcgcgactt
    23161 cagccacggc tgcatccgca tcgagcagcc ggtggcgctg gcgcagtggg tgctggcgga
    23221 cgaccccgac tggagcgagg cgcgcatccg ccagaccatg ggccagccgc gcacggtcag
    23281 cgtggcgctg cccacgccgg tgccggtgct gatcgtctac cgcacggtca gcgtgcgcga
    23341 cggccgggtg cactttgcgc ccgacctgta ccgtcaggac gcgctgctgg agcgcgcgct
    23401 ggcgcagcga ccgcaccccg cccgcggccc gggtcccgtg cgcggcgcgt cttagaatgc
    23461 tttggtttgc aacgattcag gaatccgtcg catgaacaat cctcatcgtc gccactttct
    23521 gggccgcagc ctgggcgccg cccagctggc cgtggccagc gtgctgctgc cggccggcgg
    23581 ccgggcgctg gccagcgccc ccgacaccct ggcgccggcg cgccgcctgg cgttcaacca
    23641 cctgcacacg caggagcgca cggcgctggt ctatgcggtg ggcgaggcct tcgtgcccag
    23701 cgcgctggac cacctgaacc acttcctgcg cgaccactac agcggcgacg tcggcgtgat
    23761 cgaccccgag ctgtttcacc tgctgcaccg cgtgcggcgc gagctggaga gcgagcagcc
    23821 cttccacgtc atttccggct accgcagccc gcacaccaac gaaaccctgc gcaccacgcg
    23881 cggcggcggc gtggcccggc gcagcctgca catggacggc aaggccatcg acgtgcgcct
    23941 gccgggcgtg gcgctggccg atctgcgcga cgccgccctg tcgctcaagg ccggcggcgt
    24001 gggtttctac ccgcgcgaga acttcgtgca catcgacacc gggcgcgtgc gcagctggta
    24061 ggccgcgcac acgccgtccg tcagcgcccg tccagccgcc gcagcatgcc gcgtgccgcc
    24121 cgcacgccgc tggccatggc cgcggtcagc aggtagccgc cggtgggcgc ttcccaatcc
    24181 agcatttcgc cggcgcagaa cagcccgggg gcgcagcgca actccagctc ctcggtcagc
    24241 gactccagtc gcacgccacc ggcgctgctg atggcttcgg gcagcggacg cggcgccgtc
    24301 agcgtgaccg gcagcgcctt gatggcggcc gccagcgccg ccgcatcgcg cagttgctcg
    24361 ggcgtcagca gctcgttgag cagcgccatc ttcacccccg ccaggcccag gcggctgctg
    24421 aggtgcgacg acaggctgcg gctgccgcgc gggtgcgcca ccgccgcccg cacctgcgcc
    24481 ggcgtgtggc cgggcagcag gtcgagcgcg aagctggcct gcccggcgcg cgcgatctcg
    24541 tcgcgcagca ggtggccgca ggcgtagatc aggctgcctt cgacgccgct ggccgtcacc
    24601 acgaactcgc ccgggcgcgc gaagtgccgg ccggcgccgt cggtgaaggt cagcgccacg
    24661 ctcttgagcg gctggccggc aaagcgctcg gcaaagaacg gcgtccagcc cctgcccgcg
    24721 cggccggcgg cgtcgaagcc gcagttggcc ggcgccagcg gcgccagctc ggccccgacc
    24781 tgcgccagcc agggcaccca ggcgccgtcc gagcccaggc gcggccagct cgccccgccc
    24841 agcgccagca gcgccagcgc cggcgacagg tgccgccgcc cggccggggt ttcaaagata
    24901 aatcggcctg cagcgctttt ctggcaagcg ccagaagcta ttgtttcagt agcatcggaa
    24961 gctccctccc accccaccca gcggtgccgc atgtgaaagc gcaccggcac cgcgccaggc
    25021 gtctgcgggt ggcgcaggcg ctgcagccag gcgcgcagca gcggcgcggc cttcatctcg
    25081 gtcggaaaca cccggcccga gctgcccacg aaggtgtcga tacccagccc caccgcccag
    25141 tcgcgcagcg cctgcgggcc gaagtcgtgc agccagccgg ccaccgcccc ggcgcgtgcg
    25201 ccgtagcggc cgatgaagtc gggcagcggc tcggcatgcg tcaggttcag gccgcccttg
    25261 ccggccagca ggaacttgcg acccaccgag ggcatggcgt cgtagacgtc gaccgccaca
    25321 ccggcggccg acagtcgctc ggccgccatc aggccggcgg ggccggcgcc ga
//
LOCUS       DAOQTZ010000033        25179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2304_length_25179_cov_13.981253, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000033 DAOQTZ010000000
VERSION     DAOQTZ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 25179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25179
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2304_length_25179_cov_13.981253"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKN51_05710"
     CDS             <1..500
                     /locus_tag="PKN51_05710"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNT84630.1"
                     /translation="IVSNHRDFYQLAASYNVPFHHIPVTREHKAQAEARQLEIIESED
                     AELVVLARYMQILSDGMCQRLSGRAINIHHSFLPSFKGARPYWQAHERGVKLIGATAH
                     YVTADLDEGPIIEQDVARVDHSATVEDLTAVGRDTESQVLARAVKWHGEHRVLLNGQR
                     TVVFR"
     gene            complement(560..1258)
                     /locus_tag="PKN51_05715"
     CDS             complement(560..1258)
                     /locus_tag="PKN51_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNT84631.1"
                     /translation="MTTIAAKLQQVRARIATACRQAGRNVEDVTLLAVSKTFGPQAVR
                     AAADAGQLDFGENYLQEGVDKIAALADLRGQLRWHCIGPIQSNKTRLVAEQFDWAHGI
                     DRLKTAERLSAQRPPGLPPLNLCLQVNIDGGPTKSGVAPAEALALARAVAALPRLRLR
                     GLMTIPEPAPDFEAARAVHASARALFDQMNAAGLGLDTLSMGMSDDLEAAIAAGSTLV
                     RVGSAIFGARPPKT"
     gene            1304..2347
                     /locus_tag="PKN51_05720"
     CDS             1304..2347
                     /locus_tag="PKN51_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNT84632.1"
                     /translation="MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRLNVDPLDH
                     KQVFAMLYDIMNDTQRKIYEENLEVDFSFEIEGLARFRVNAFNQNRGAAGVLRTIPSK
                     ILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNEMEYGHVLTVED
                     PIEFVHESKKCLINQREVGPMTHSFANALRSALREDPDAILVGEMRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTIDRIIDVFPAEEKEMVRAMLSESLVAVISQTLCKLKDGSG
                     RVAAHEIMLGTPAIRNLIREAKVAQMYSSIQTGNSLGMQTLDQNLTDLVRRNVISAAE
                     ARSKAKIPENFPG"
     gene            2485..3621
                     /locus_tag="PKN51_05725"
     CDS             2485..3621
                     /locus_tag="PKN51_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNT84633.1"
                     /translation="MERDQASKFIHDLLKLLLTRKGSDLFITADFPPAIKVDGKITKV
                     SSQNLTAAHTLALVRAVMNDKQAAEFERTKECNFAISPAGIGRFRCNAFIQQGNIGMV
                     MRVIPAELPTIDGLGMPQILKEVAMSKRGLTILVGATGSGKSTTLAAMVDWRNINSQG
                     HIITVEDPIEFVHPHKNCIVTQREVGLDTEDWEAALKNSLRQAPDVILMGEIRDRETM
                     EHAVAFAETGHLCLATLHANSANQALDRIINFFPEERRQQLLMDLSLNLRALISQRLL
                     PKQDAKGRTAVVEIMLNSPLISDLIFKGEVGEIKEVMKRSRELGMQTFDQALFDAFED
                     NLITYEDALRNADSANDLRLQIKLTSKRAKERDLAAGTEHLQIV"
     gene            4031..4948
                     /locus_tag="PKN51_05730"
     CDS             4031..4948
                     /locus_tag="PKN51_05730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT84634.1"
                     /translation="MNTTIHARSYDPTPARRVAFLGLGVMGYPMAGHLALAGHEVTVY
                     NRNATKSEAFCAQLAGSGTVKRAQTPAAAAAGAEIVLACVGNDDDLRQVVLGEHGAFA
                     GMAPGAVFVDHTTASATVARELDALARSRGLHFIDAPVSGGQAGAQNGQLTVMCGGDP
                     AAFERVRPVAMAFSRAFTLMGPSGAGQLTKMVNQICIAGLVQGLSEAIAFGQRAGLDM
                     AQVIEVIGKGAAQSWQLDHRGPTMVQDRFDFGFAVDWMRKDLGLVLDEAKRNGARLPV
                     TALVDQFYADVQQLGGGRWDTSSLIRRLK"
     gene            complement(4984..5721)
                     /locus_tag="PKN51_05735"
     CDS             complement(4984..5721)
                     /locus_tag="PKN51_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNT84635.1"
                     /translation="MKSFCMRGVGMALAAALLSGCAAAVVGGAVGTGVLLSSADRRSG
                     DTQQADQTIEAAAREAVAQTLAGRGHVNVASYYRKTLITGEVGSAQDRLQVEAQVRAI
                     PGVQGVVNELAVMPESSAFQRSNDAFITSKVRSRLVGTNGVPSGALKVLTERGTTYLM
                     GRLTATELALATDAVRQTEGVQRVVRVVDLIVEPASGALAPPVQGATPPAPATEAANG
                     VQTHPVTQPAIVHAPAPIQVQTLPPVK"
     gene            complement(5718..6317)
                     /locus_tag="PKN51_05740"
     CDS             complement(5718..6317)
                     /locus_tag="PKN51_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNT84636.1"
                     /translation="MPEPRTSQQFIDSADLKYQWAQTLARPIDEATQALLACITGGGK
                     LMVCGNGASGALAQYLVALLVGGLERARPELPALALGADAATLTAVARRGEYADVFAA
                     QVRALGNPGDALLLLSTNGNTVNLVRAAQAAHERDMCVIALSGRGGGELGRMLRDTDV
                     HVCVPHERTPRIHEAHQLVLHCLCDGLDLQLLGHEESSE"
     gene            complement(6416..6805)
                     /locus_tag="PKN51_05745"
     CDS             complement(6416..6805)
                     /locus_tag="PKN51_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNT84637.1"
                     /translation="MRGWGGRQQPGASTRVRGAAAEDMALAHLLRAGLRLIERNYRTP
                     GRGGGEIDLVMRAPDGTLVFVEVRARASAAHGGAAASVGAVKQRRIVLAARHYLLRFG
                     APPRCRFDVVAIEGTHIDWLPAAFDAG"
     gene            6824..7747
                     /gene="rsmI"
                     /locus_tag="PKN51_05750"
     CDS             6824..7747
                     /gene="rsmI"
                     /locus_tag="PKN51_05750"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNT84638.1"
                     /translation="MSDSAPVGKADLHAALAAAHRAAGAQHYPAGSLYVVATPIGNLA
                     DITLRALHVLALADAIACEDTRHTCQLLRAYGIDGKPLLALHEHNEREAAATVIARLR
                     AGERVAYVSDAGTPAVSDPGARLVAACAAEGLRVVPLPGASSVTAALSAAGLAGDGQF
                     LFRGFLPARASERQAAVQALAAEPRAVVLLEAPHRIAALADALAALDQRAITVARELT
                     KQFEQLATMACAHLPRWLASEPQRQRGEFALVLHPAPDTPGPGDGERVLRLLLSELPL
                     KTAVKLAAELSGAPRNALYQQALRWQGEPGR"
     gene            complement(7918..8712)
                     /locus_tag="PKN51_05755"
     CDS             complement(7918..8712)
                     /locus_tag="PKN51_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNT84639.1"
                     /translation="MTEALTPPPEPAEPAADPRADTLEALEGALDALREHHPQRVPQW
                     EYCEGVLTALLCMRRTVPPTEWLPLLFDGDDVFASEGERTQFMMHWLAREAQLRAQLQ
                     APVEALDDEQALEPGVIDWRGLLASLPEDERAAATAEGTPPPIAQAWAGGFIDAVDYW
                     AEDWAPPRDKEIAAQMDDALDCIATLLEEDREAPKFNLFDEHGAPSVSQARFEAFGEA
                     IWAVYDLFDIARSLGPRIAPARSDKVGRNDPCPCGSGKKFKKCCGT"
     gene            complement(8756..9661)
                     /locus_tag="PKN51_05760"
     CDS             complement(8756..9661)
                     /locus_tag="PKN51_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT84640.1"
                     /translation="MSERLSARAAAGFATLLLIALLMAANHVSARLAFQHGVDVPTAV
                     TVRSLGTALVVGLLLHWQQVPLALNARQRKALPLIGALIAVQSLWLYSAVARLPVALA
                     LLVFNSFPLMTALWARLLYQHRPQRAVLVAMPVLLLGLLLALDVLGAASGLGAAGQWR
                     QIGVGVAFALGAAATFGLALVLTQHEAGALDGRLRTLATMTIVGLLAGAVALARGGLA
                     LPQATPGWWGLALLTLLYGTGITLLFTVLPRLGVVGHSAIMNVEPVFALILAWLVLGQ
                     AVAPVQVAGALVVVGAVVWLGLWRR"
     gene            complement(9654..10652)
                     /locus_tag="PKN51_05765"
     CDS             complement(9654..10652)
                     /locus_tag="PKN51_05765"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNT84641.1"
                     /translation="MFHKICIYGAGAIGGWLGARLAAQGAAVSAVARGATLAALRAHG
                     LRLREAGDDGAVIETVHAVQASEQPAELGVQDLVIVAVKAPAMGQVAAHIAPLLGPDT
                     VVLTAMNGVPWWFLQGFGGALAGTRLESVDAGGSIAAAIEARRVIGAVVHASCSVDAP
                     GVIRRHFGNGLIVGEPGGGDTPRLAALAALLRQARFEVTVSPQIQKDVWYKLWGNMTI
                     NPISALTGASADRILDDDLVRGFVSAVMLEAREIGARIGVPIEQTPDDRHQVTRKLGA
                     FKPSMLQDVEAGRPVEIDALVGAVRELGQLTGVATPCTDALLGLARLHARVRGLYV"
     gene            complement(10816..11244)
                     /locus_tag="PKN51_05770"
     CDS             complement(10816..11244)
                     /locus_tag="PKN51_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNT84642.1"
                     /translation="MSPQLHVAEPPAPYLARPALVIDCSVLAGLVFREHWYEAARDQV
                     HGSTPHAPHLLIYEIGNVALKKHRRQEAHALPGLAHALTLDIELHEVDMAQTFALAQR
                     YQLSAYDASYLWLAGALRCPLATFDETLAQAAQQHLGALG"
     gene            complement(11241..11573)
                     /locus_tag="PKN51_05775"
     CDS             complement(11241..11573)
                     /locus_tag="PKN51_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNT84643.1"
                     /translation="MTTLTIKDVPEPLAQQLRQRAARNHRSLQGELMSIIEQAVQQGD
                     QPGAGLIGTRTLGGGTIVGMSAQGPIVRRGYKTIEQIYDELRTKYPEPIFDQSDSVQL
                     IREDRDSR"
     gene            complement(11651..>12372)
                     /locus_tag="PKN51_05780"
     CDS             complement(11651..>12372)
                     /locus_tag="PKN51_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNT84644.1"
                     /translation="RLQLAACYRVFALLGWTEMIYNHITLRVPASATGGEKHFLINPF
                     GLHYSEVTASNLVKVNLAGDILSESRYPINPAGYVLHSCIHDGVPGAHCVMHTHTTAG
                     VAVASLASGLSQSNFYSAQLHDMVAYHDFEGITIRADEGPRVVASIGQRKAVILRNHG
                     LLSWGETLPLAFAVLWTLQRACEIQLATQSMGAPLPVPEGIAAACTRDSFQFDPARGA
                     GQDVFDALVRQVDRVDGSWRQ"
     assembly_gap    12373..12472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12564..13412)
                     /gene="yghU"
                     /locus_tag="PKN51_05785"
     CDS             complement(12564..13412)
                     /gene="yghU"
                     /locus_tag="PKN51_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HNT84645.1"
                     /translation="MSSAYVPPKVWRWEPKEDNKFANINRPTAGARFEQELPVGQHPL
                     QLYSLATPNGQKVTILLEELLAAGHAGAEYDAWLIDIGEGAQFGSGFVAINPNSKIPA
                     LLDRSVQPAIPVFESGAILLHLAEKFGAFIPTAPAARAACLSWLFWQMGATPYLGGGF
                     GHFYAYANDKQQYPIDRFTMEVKRQLDLLDRQLAEHEYVAGSDYSIADMAIWPWYGAL
                     VVGEAYNAAEFLEAATYKNVLRWQQQIAARPAVQRGRLVNRRNGGLRERHAASDFDGV
                     WPKAAP"
     gene            complement(13461..14252)
                     /locus_tag="PKN51_05790"
     CDS             complement(13461..14252)
                     /locus_tag="PKN51_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HNT84646.1"
                     /translation="METPLNPFKQALHEKRRQIGFWLNLPNTTVAEIAAGAGFDWLLI
                     DGEHTYHSLETILHQLQTIAAYPRTHAIARLPMGHGHVGEMLIKQYLDLGVQTLLVPM
                     VDTPEQAAAIVRAARYPGGGGAGGIRGMAGGRAARWGRYPKYVHQANDRVCILVQAET
                     RTALDNLEAIAAVDGVDGIFIGPADLSASMGHRGNPAHPEMQAIMEDATRRIVRAGKA
                     AGNLSPDAATAQRYLEWGATFVAVGLDTNVLARETSALAQKFAAG"
     gene            complement(14255..15070)
                     /gene="hpaH"
                     /locus_tag="PKN51_05795"
     CDS             complement(14255..15070)
                     /gene="hpaH"
                     /locus_tag="PKN51_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HNT84647.1"
                     /translation="MLTDEQIQQLAAELHASEKSRQQVEHFSKRFPGMTIEDGYRISR
                     AWVALQRAEGRQVIGHKIGLTSRAMQISSQIDEPDYGTLLDSMLYTCTPGQVLGIPTD
                     RFIAPRVEVELAFVLKADLAGPHVDVEQVLAATDYVTPAIEIIDARIEQFDRHTKVMR
                     KVYDTISDNAANAGIVVGAGRADARTIDRAWVGAILRQNGAVEETGLAAGVQGDPAIG
                     IAWLANKLAPWGEVLRAGQIVLAGSFTRPVAAKRGDAFEADYGPLGQLQFKFV"
     gene            complement(15072..15530)
                     /locus_tag="PKN51_05800"
     CDS             complement(15072..15530)
                     /locus_tag="PKN51_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HNT84648.1"
                     /translation="MPHLVILYSGNLDAIVDMPALCRDAADAMLAVRDEAGAQVFPTG
                     GTRVLAYPAPHFALADGGAAGRAAGAGKTGITGSDPGEYGFMYANLRMARGRSSATQQ
                     AAGEAIARALRARLDPCMATRHIGLTVQVDEGPEVFDAKHSTLHPLFRKA"
     gene            complement(15543..>16396)
                     /gene="hpaD"
                     /locus_tag="PKN51_05805"
     CDS             complement(15543..>16396)
                     /gene="hpaD"
                     /locus_tag="PKN51_05805"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HNT84649.1"
                     /translation="RQGTRQDAIDGHKEIGRRCRELGVDSIVVFDTHWLVNATYHLNC
                     APRWQGSYTSNELPHFISNMAYGFNGNPELGRLLAQVCNEQGVETLAHDATSLQPEYG
                     TLVPMRYMNADEHFKVVSVSALCTSHYLNDSARLGWAMREAVEKHYDGKVAFMASGSL
                     SHRFAQNGLAPEFAFKIWSPLLERLDHEVIQMWKNGEWQDFCAMLPEYATKGHGEGMM
                     HDTAMLLGALGWSAYDGKMEVITPYFAASGTGQMNAVFPVTPQDGHAVPPPEASRATG
                     FRAFARI"
     assembly_gap    16397..16496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16555..17496)
                     /locus_tag="PKN51_05810"
     CDS             complement(16555..17496)
                     /locus_tag="PKN51_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNT84650.1"
                     /translation="MSAGKLTAAVVQASSVLLDTPATVERALGLMAEAARQGAQLVVF
                     PEAFIGGYPKGADFHIYLGARTPQGRAEYKLYYDAAVSVDGPELAQLAEACARHQMYA
                     VFGIIEREGGTLYCTAVYLGPQGRVIGKHRKLMPTALERLVWGFGDGSTLQAVDTPWG
                     KLGAVICWENYMPALRLAMYQQRVAIYCAPTADDRDSWQSTMQHIALEGRCFVLSACQ
                     HLRRRDFPGERMNNRLPEAPDTVLMRGGSSIHDPLGQRLAGPVFGEDALLTAELDLSI
                     IPAAQMDFDPVGHYARPDVFSLHVDTAPQRAVQLGDQ"
     gene            complement(17576..18541)
                     /locus_tag="PKN51_05815"
     CDS             complement(17576..18541)
                     /locus_tag="PKN51_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT84651.1"
                     /translation="MQRRYLIQAAAAVALAPSMARAQSPWPSKPIRIVVPFTPGGTTD
                     FVTRLVATELARSLGQPVMIENKPGAGTVIGVDNVAKSAPDGSSFVTVANSFTVNQTL
                     VKKLPYDGLKDLRPVALMGLSEHVLATHPGSGLKTVADIVAQAKAGKKLSYASFGNGT
                     SAHLSGEMLKTQLGVDIVHVPYKGQSPALADLLGGQVSMMFGNWPEFRNHVQSGKLVA
                     IGMATAQRSTYAPDIPTLAEQGLKIESNSWNGLLAPAAVPDAIVQRMNAAVNEAMKQP
                     AVVEAFHKGGIAARSGTPQQFADFIASETARYAEVIRRAGISADG"
     gene            complement(18546..20015)
                     /gene="hpaE"
                     /locus_tag="PKN51_05820"
     CDS             complement(18546..20015)
                     /gene="hpaE"
                     /locus_tag="PKN51_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HNT84652.1"
                     /translation="MKIDHLIDGKAVASSDCFESTNPATQGVLAEVASGGEAEVNAAV
                     AAAKAAFPKWAGLPAPERAKKMRALGALIAKHVPELARTETDDTGQVIAQTGKGLIPR
                     AADNFDYFAEMCVRVDGHTYPTPTHLNYTLFHPVGVCALISPWNVPFMTATWKVAPCL
                     AFGNTAVLKMSELSPLTAARLGELALEAGIPPGVLNIVHGFGKEAGEPLCAHPDVRAI
                     SFTGSTATGNRIVRAAGLKKFSMELGGKSPFVIFDDADLDRALDAAVFMIFSNNGERC
                     TAGSRILVQQSIYADFAQKFAERAKKITVGDPQDETTIVGPMISKGHLAKVRSYIELG
                     PKEGATLLCGGLDRPSYAPELGARVKDGNFVWPTVFADVDNRMRIAQEEIFGPVACLI
                     PFKDEQDAIAKANDIQYGLSSYVWSENIGRAHRVAAAIEAGMCFVNSQNVRDLRQPFG
                     GTKASGTGREGGTWSYEVFLEPKNIAVSMGSHHIPHWGV"
     gene            complement(20022..20819)
                     /locus_tag="PKN51_05825"
     CDS             complement(20022..20819)
                     /locus_tag="PKN51_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNT84653.1"
                     /translation="MKHARIAWAGAVHEAVESEGQLELLTPAYRGRRLSFDEVVWLPP
                     LAPVPRARTVLALGLNYADHARELAFKPPEEPLAFAKGAASLIGHRAHTVRPDGVQNM
                     HYECELAVVIGRSAKKVRKDDAYDYVAGYTVANDFAIRDYLENWYRPNLRVKNRDTCT
                     PIGPWLVDAADVPDPMKLRLSTHVNGQLTQQGTTADMVFDVPTLIEYFSSFMTLNPGD
                     LILTGTPDGVVDCPVGSVVVCEIEGLGALQTTMVSESNRAAAPASQA"
     gene            complement(20816..21604)
                     /locus_tag="PKN51_05830"
     CDS             complement(20816..21604)
                     /locus_tag="PKN51_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNT84654.1"
                     /translation="MNPDLPWQPAGTVYGPLLNLRREHALWAPRMGQAPYQAAPKAPV
                     LYVKTANTLAACGAAVAVPQPVWLGPTLGLVVGDHASNWPTARNQQAPAAIKTIVLGG
                     VAIAGCVLLNDLSLPHDSYYRPAIRFRNRDGFLVCGTPAAPLAPAQIAALQIDARVNG
                     ALVQTVDLATLVRDAATLLADVSAFMTLQPGDVLMLGTDCLADGSRPLAQAGDRVEVS
                     APGFAPLVHTVVAESSAAGPPQGARAPSGGSAGAPAPSVGARSS"
     gene            complement(21608..22084)
                     /gene="hpaR"
                     /locus_tag="PKN51_05835"
     CDS             complement(21608..22084)
                     /gene="hpaR"
                     /locus_tag="PKN51_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HNT84655.1"
                     /translation="MPRPTPFVHRNLPLLLLRAREALMQHYRPGLRAHGLSDQQWRVL
                     RVLREHEGGLETGRLAQQAYILGPSLTGMLARMEKSGLVARQRCPDDARRSLVAATPD
                     GLALADALSDAIEAQYQDLAAHLGQARLNQLYTLLDELIALPSHADAEPVEALMEP"
     gene            22138..23184
                     /locus_tag="PKN51_05840"
     CDS             22138..23184
                     /locus_tag="PKN51_05840"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNT84656.1"
                     /translation="MTGQTLSSRLATRWPLLQAPMAGAQDSAMALAVSGAGGLGALPC
                     AMLAPDALRRELQTLAASGLPYNVNWFCHPPPAPDAAREAAWRAALKPYFDELGLDPE
                     AVPGGPGRAPFSREAAELMAEFRPPVVSFHFGLPAPELLTRVKSWGAFVLSSATTVRE
                     ALWLQEHGADAIIAQGLEAGGHRGHFLSDDLSEQMGTFALLPQVVAAVRVPVIAAGGI
                     ASAAGVRAAQALGAAGVQVGTAYLCADEALTTPAHRAALHSEAARHTVITNLVTGRPA
                     RGMVNRVMRELGPISPAPSAFPLATAAMAYLRAAAEKQGRHDFTHWWSGQNASGCRAA
                     PAADITRALAQGFA"
     gene            23384..23938
                     /locus_tag="PKN51_05845"
     CDS             23384..23938
                     /locus_tag="PKN51_05845"
                     /inference="COORDINATES: protein motif:HMM:NF022664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HNT84657.1"
                     /translation="MSRIAHLLSGIAFAATTLGLTTPAQAQQTIECRSNNYGYNECYA
                     GDLSSPQLIHQTSSSACIVNRSWGFNPRRGYIWVNEGCSGVFADVGGYHHGRGDTWDQ
                     GARRYDDRGHDAGAIVGGLVLGAMLGAAAGDDHDAHRHARDRRSEEIDTRPQFDRDGN
                     PNFDVHGNYQGCHGLGCQVDNPDR"
     gene            23984..24424
                     /locus_tag="PKN51_05850"
     CDS             23984..24424
                     /locus_tag="PKN51_05850"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HNT84658.1"
                     /translation="MTRTNRVLFRLAAGLTTALALQAVHASDDPCFEKAQSQAALNQC
                     ASAAYQRADADLNRLYQLMTARLSTDDAARKRLVDAQRKWLQFRDAECAFQTVRTVGG
                     SVQPMNVSSCLTDLTRERVTDLQKHLDCARVMGEGGACAVPPRR"
     gene            24476..25075
                     /locus_tag="PKN51_05855"
     CDS             24476..25075
                     /locus_tag="PKN51_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84659.1"
                     /translation="MPSTAVLSHDIYPHIRVVLGMVIGLGVTRLLSGLARLVQHPGQH
                     RMDAVHLGWVGSTLLALVHFWWWQFGLYQIEAWTFGTYLFVIGYAIALFLLAALLFPD
                     SMAGYEGYGDYFQERRAWFFGVLASTYLLDVIDTLLKGDAHFARFAREYLIRTPILVA
                     LCMVAARTARRRFQVAFVALVLIYQLSWIFRLFNTLQQA"
BASE COUNT         3799 a         8719 c         8585 g         3876 t
ORIGIN      
        1 ccatcgtcag caaccaccgc gacttctacc agctggcggc cagctacaac gtgccgtttc
       61 accacatccc cgtcacgcgc gagcacaagg cgcaggccga ggcgcggcaa ctggagatca
      121 tcgagtccga agacgccgaa ctggtggtgc tggcgcgcta catgcagatc ctgtcggacg
      181 gcatgtgcca gcggctttcc gggcgcgcca tcaacatcca ccacagcttc ctgcccagct
      241 tcaagggcgc gcggccatac tggcaggcgc acgagcgcgg cgtcaagctg atcggcgcca
      301 ccgcccacta cgtgacggcc gacctcgacg aaggtccgat cattgagcag gacgtggcgc
      361 gcgtggacca cagcgccacg gtggaagacc tgaccgccgt cggccgcgac accgaaagcc
      421 aggtgctggc gcgcgccgtc aaatggcacg gcgagcaccg ggtgctgctc aacgggcagc
      481 gcacggtggt gttccgctga ggccggccag cgggccgatg ccgccgaagc cgggcctgag
      541 ggggttggtt caccccctct caggtcttgg gcgggcgggc gccgaagatc gcgctgccca
      601 cccgcaccag ggtcgagccg gcggcgatcg ccgcttccag atcgtcgctc atgcccatcg
      661 acagcgtgtc cagccccagg ccggccgcat tcatctgatc aaacagggct ctggcgcttg
      721 cgtggactgc gcgagcagct tcaaaatcag gagcaggttc ggggatcgtc atgagcccgc
      781 gcaggcgcag tcgcggcagc gcggcgacgg cgcgcgccag cgccaacgcc tcggccggcg
      841 ccacgccgga cttggtcggc ccgccgtcga tgttgacctg caggcacagg ttcagcggcg
      901 gcaggccggg cgggcgctgg gccgacaggc gctcggcggt cttcagccgg tcgatgccgt
      961 gggcccagtc aaactgctcg gccaccaggc gggtcttgtt gctctggatc gggccgatgc
     1021 agtgccagcg cagctggccg cgcagatcgg ccagggcggc gatcttgtcc accccttcct
     1081 ggaggtagtt ctcgccgaaa tcgagctgcc cggcatcggc ggccgcgcgc acggcctgcg
     1141 ggccgaaggt cttggacacg gccagcagcg tgacatcttc cacgttccgc ccggcctgcc
     1201 ggcaggctgt ggcgatccgt gcacgcacct gctggagctt ggcggcgatc gtcgtcataa
     1261 tgcccgcagc gtatcaaact gcaaccgttt cggaccgatt cccgtggaca tcacccaact
     1321 gctggctttc agtgtcaaga acaaggcgtc ggacctgcac ctgtccgccg gcctgccgcc
     1381 catgatccgc gtgcacggcg acgtgcgccg gctgaacgtc gacccgctcg accacaagca
     1441 ggtgttcgcg atgttgtacg acatcatgaa cgacacccag cgcaagatct acgaagaaaa
     1501 cctggaggtc gacttctcgt tcgaaatcga gggcctggcg cgctttcgcg tcaacgcctt
     1561 caaccagaac cgcggcgccg ccggggtgct gcgcaccatt cccagcaaga ttctgacgct
     1621 ggagcagctc aatgcgccca agatcttcgg cgatctggcg ctcaagccgc gcgggctggt
     1681 gctggtcacc ggccccaccg gttcgggcaa gtcgaccacg ctggcggcca tggtcaacca
     1741 cctcaacgaa atggagtacg gccacgtgct gacggtggaa gacccgatcg agttcgtgca
     1801 cgaatccaag aaatgcctga tcaaccagcg cgaggtgggc cccatgacgc acagcttcgc
     1861 caacgcgctg cgctcggccc tgcgcgagga cccggacgcc atcctggtgg gcgagatgcg
     1921 cgacctggaa accatccgcc tggccatgac cgccgccgaa accggccacc tggtgttcgg
     1981 tacgctgcac accagcagcg ccgccaagac catcgaccgc atcatcgacg tcttcccggc
     2041 cgaggaaaag gaaatggtgc gcgccatgct gtcggagtcc ctggtggcgg tgatttcgca
     2101 gacgctgtgc aaactcaagg acggctcggg ccgcgtggcg gcgcacgaaa tcatgctggg
     2161 cacgccggcc atccgcaatc tgatccgcga ggccaaggtg gcgcagatgt actcttcgat
     2221 ccagaccggc aacagcctgg gcatgcagac gctggaccag aacctgaccg acctggtgcg
     2281 ccgcaacgtc atcagcgcgg ccgaagcgcg cagcaaggcc aagattccgg aaaacttccc
     2341 cggctgacta ccgtgccccc acgccccgct gccgcccatg ctgcgccgcc cgccaggggg
     2401 gcccggccag cctgcgcgcg gcgcggcagc gaccgggcgg ctccggctgc acgcgcaaac
     2461 ccgaagtaga ggacgtcaac gaccatggaa cgcgatcagg ccagcaaatt catccacgat
     2521 ctgctcaagc tgctgctcac gcgcaagggc agcgacctgt tcatcacggc cgactttccg
     2581 cccgccatca aggtggacgg caagatcacc aaggtatcgt cacagaacct gaccgcggcc
     2641 cacaccctgg cgctggtgcg cgcggtgatg aacgacaagc aggcggccga gttcgaacgc
     2701 accaaggagt gcaacttcgc catctcgccg gccggcatcg gacgctttcg ctgcaacgcc
     2761 ttcatccagc agggcaacat cggcatggtg atgcgggtga tcccggccga gctgccgacc
     2821 atcgacggcc tgggcatgcc gcagatcctc aaggaagtgg cgatgagcaa gcgcggcctg
     2881 accatcctgg tgggtgccac cggctcgggc aagtcgacca cgctggcggc catggtggac
     2941 tggcgcaaca tcaacagcca gggccacatc atcacggtgg aagacccgat cgaattcgtg
     3001 cacccgcaca agaactgcat cgtcacccag cgcgaggtcg ggctggacac cgaagactgg
     3061 gaagcggccc tgaagaacag cctgcgccag gcgcccgacg tcatcctgat gggcgagatc
     3121 cgcgaccgtg aaaccatgga gcacgccgtc gcctttgccg aaaccggcca cctgtgcctg
     3181 gccaccctgc acgccaacag cgccaaccag gcgctcgacc gcatcatcaa cttcttcccc
     3241 gaagaacggc gccagcagct gctgatggac ctgtcgctca acctgcgggc cctgatctcg
     3301 cagcgcctgc tgcccaagca ggacgccaag ggtcgcacgg cggtggtcga gatcatgctc
     3361 aactcgccgc tgatcagcga cctgatcttc aagggcgaag tgggcgagat caaggaagtg
     3421 atgaagcgca gccgcgagct gggcatgcag accttcgacc aggcgctgtt cgacgccttc
     3481 gaggacaacc tcatcaccta cgaagacgca ctgcgcaacg ccgactcggc caacgacctg
     3541 cgactgcaga tcaaactcac cagcaagcgc gccaaggagc gcgacctggc ggccggcacc
     3601 gaacatctgc agatcgtctg aacctaggag gctgtcggac ttggaaatcg ccggctgcaa
     3661 atcgaccgca gcggcccatt tttcaccggc tttttgccca atagctcgac tattgggctg
     3721 caaatccggc aaaaactggt ctcgctgggg ccgattctcg ctttcggcgc tccaagcccg
     3781 atagcctcct aggaggctga accgaacgcc gcggtgggcc gcgggcacgc ttcgccaccg
     3841 ccgcatcccc gcagcgtcgc gcggcttcga cggccttcat ctgcggggtg agcacgctgc
     3901 cgacttgccc gcaccggcgg ctggccccac agctcgtcca tggggctgca agccctggcc
     3961 acgcgggtct cgccggtgcc gatgctggct tttggtgcgc caggtgcgac aggctcctaa
     4021 aatttgccgc atgaacacca ccatccacgc ccgcagctac gaccccaccc cggcccgccg
     4081 tgtggccttt ctcggcctgg gcgtgatggg ctaccccatg gctggccacc tggcactggc
     4141 cggccacgag gtcaccgtgt acaaccggaa tgctacaaaa tcagaagcgt tctgcgcaca
     4201 attggcgggc tccggcaccg taaaacgtgc acagactccg gcggccgcgg cggccggtgc
     4261 cgagatcgtg ctggcctgcg tgggcaacga cgacgacctg cgtcaggtgg tgctgggcga
     4321 gcatggcgcc tttgccggca tggcgccggg ggcggtgttc gtcgatcaca ccaccgcctc
     4381 ggccaccgtg gcgcgcgagc tggacgcgct ggcgcgcagc cgcggcctgc acttcatcga
     4441 cgcgccggtc tcgggtggcc aggccggggc gcagaacggc cagctgaccg tgatgtgcgg
     4501 cggcgatccg gcggcgttcg agcgcgtgcg gccggtggcc atggcgttct cgcgcgcctt
     4561 caccctgatg gggccaagcg gcgccgggca gctgaccaag atggtcaatc agatctgcat
     4621 tgcggggctg gtgcagggcc tgtcggaggc gatcgccttc ggccagcgcg ccggcctgga
     4681 catggcgcag gtgatcgagg tcatcggcaa gggcgcggcg cagagctggc agctcgacca
     4741 ccgtggtccg accatggtgc aggaccgctt tgatttcggc tttgcggtgg actggatgcg
     4801 caaggacctg gggctggtgc tggacgaagc caagcgcaac ggcgcccgcc tgccggtgac
     4861 ggcgctggtc gatcagttct atgccgacgt gcaacagctg ggcggcggcc gctgggacac
     4921 ctccagcctg atccggcgcc tgaagtaacg cccgcacctg ggaccgcccc aggccccgac
     4981 cgtctacttg accgggggca gcgtctgcac ctggatcggt gccggcgcgt ggacgatggc
     5041 cggctgcgtg accgggtggg tctgcacgcc gttggccgcc tccgtggccg gcgccggcgg
     5101 ggtggcaccc tggaccggcg gcgccagcgc gccggatgcc ggctcgacga tcagatcgac
     5161 cacccgcacc acgcgctgca cgccttcggt ctggcgcacg gcgtcggtgg ccagcgccag
     5221 ttcggtggcc gtcaggcggc ccatcaggta ggtcgtgccg cgctcggtca gcaccttgag
     5281 ggcgcccgag ggcacgccat tggtgcccac caggcggctg cgcaccttgc tggtgatgaa
     5341 ggcgtcgttg gagcgctgga aggcgctgga ttcgggcatc accgccagct cattgaccac
     5401 gccctgcacg cccgggatgg cgcgcacctg cgcttccacc tgcagtcgat cctgcgcgct
     5461 gccgacttcg ccggtgatca gggtcttgcg gtagtagctg gcgacgttga catggccgcg
     5521 ccccgccagc gtctgggcca cggcctcgcg cgccgccgcc tcgatcgtct ggtcggcctg
     5581 ctgggtgtcg ccactgcggc ggtcggccga actcagcagg acaccggtgc ccaccgcacc
     5641 acccaccacc gccgccgcgc agccgctcag cagggcggcg gccagcgcca tgcccacccc
     5701 gcgcatgcag aacgatttca ttcggaactc tcctcgtgac ccagcagttg cagatccagg
     5761 ccatcacaca ggcaatgcag caccagctgg tgcgcctcat ggatgcgcgg cgtgcgctcg
     5821 tgcggcacgc acacatgaac gtcggtgtcg cgcagcatcc gacccagctc gccgcccccg
     5881 cgcccgctga gcgcgatcac gcacatgtcg cgctcgtgcg cggcctgcgc ggcgcgcacc
     5941 aggttgaccg tgttgccgtt ggtcgacagc agcagcagcg cgtcgcccgg gttgcccagc
     6001 gcgcgcacct gggccgcgaa gacatcggcg tattcaccgc gccgcgccac cgcggtcagg
     6061 gtggcggcat cggcgcccag cgccagtgcc ggcagttcgg gacgggcgcg ctccagaccg
     6121 cccaccagca aggccaccag gtactgcgcc agcgcgccgg acgcgccatt gccacacacc
     6181 atcagcttgc cgccgccggt gatgcaggcc agcagcgcct gcgtggcctc gtcgatgggc
     6241 cgcgccaggg tctgcgccca ctggtacttc aggtcggcgc tgtcgatgaa ctgctgtgac
     6301 gtgcggggtt cgggcatcgg cgtcgatcat aaccgcgccg ccccgccacg gtgcaaattt
     6361 ggtagcaagc aggtgttgcg gcgagggcgg cgacccgggc cggccggcgc ccgcctcagc
     6421 cggcatcgaa ggccgccggc agccagtcga tgtgcgtccc ctcgatcgcc accacgtcga
     6481 atcggcagcg cggcggcgcg ccgaagcgca gcaggtagtg gcgcgccgcc agcacgatgc
     6541 gccgctgctt gaccgcgccc acgctggccg ccgccccgcc atgcgcggcg ctggcgcgcg
     6601 cgcgcacctc gacgaacacc agcgtgccgt cgggtgcacg catcaccaga tcgatttcgc
     6661 cgcccccgcg ccctggggtt cgataattgc gctcgatcag gcgcaagccg gcgcgcagca
     6721 ggtgcgccag cgccatgtct tcggcggcgg ctccgcgcac gcgtgtgctg gctcccggct
     6781 gctggcgccc gccccaccca cgcatcacgt caaggccccg tccatgtctg attccgcccc
     6841 cgtcggcaaa gccgaccttc atgccgccct ggcggcggcg caccgggccg ccggcgcgca
     6901 gcattatccg gcgggcagcc tgtacgtggt ggccacaccg atcggcaacc tggccgacat
     6961 caccctgcgc gcgctgcatg tgctggccct ggccgacgcc atcgcctgcg aagacacgcg
     7021 ccacacctgc cagctgctgc gcgcctacgg catcgacggc aagccgctgc tggcgctgca
     7081 tgaacacaac gagcgcgagg ccgccgccac cgtgatcgcg cgcctgcggg ccggcgagcg
     7141 cgtggcctac gtcagcgacg ccggcacgcc ggcggtcagc gacccgggcg cgcgcctggt
     7201 ggcagcctgc gccgccgagg gtctgcgggt ggtgccgctg ccgggcgcca gcagcgtgac
     7261 cgcggccctc agcgccgccg gtctggcggg cgacggccag ttcctgttcc gcggatttct
     7321 gcccgccaga gcgagcgagc gccaggccgc ggtgcaggcg ctggccgccg agccgcgtgc
     7381 cgtggtgctg ctggaggcgc cgcaccgcat cgccgcgctg gccgacgcgc tggccgcgct
     7441 ggaccagcgc gccatcaccg tggcacgcga gttgaccaaa cagttcgagc agctcgccac
     7501 catggcctgc gcccacctgc cacgctggct ggcgtccgag ccgcagcgcc agcgcggcga
     7561 gttcgcgctg gtgctgcacc ctgccccaga caccccaggg cctggcgatg gcgaacgcgt
     7621 gctgcggctg ctgctctccg aactgccgct gaagaccgcc gtcaagctgg cggccgagct
     7681 gtccggggca ccgcgcaacg cgctgtatca gcaggccctg cgctggcagg gcgaaccggg
     7741 ccgctgagcc aaagaacggc cagtggccgg gctgccgttg ccgtgatcgg cgaacctggc
     7801 atggctggcg ccgcagctcc cgaaccgccg gcgtggcctg gggtggcgca ccagtgtggc
     7861 atcgtcgcgg tcattgcgct tatccagcaa gcgccaacag ctattgttca gatagcatca
     7921 ggtgccgcag cactttttga acttcttgcc gctgccgcag gggcaggggt cgttgcggcc
     7981 caccttgtcg ctgcgggccg gggcgatgcg cgggcccagg ctgcgcgcga tgtcgaacag
     8041 gtcgtacacc gcccagatcg cttcgccaaa ggcttcgaag cgcgcttggc tgacgctggg
     8101 cgcgccgtgc tcgtcgaaca ggttgaactt gggcgcctct cggtcttcct ccagcaacgt
     8161 ggcgatgcag tccagcgcgt cgtccatctg ggcggcgatt tccttgtcgc gcggcggcgc
     8221 ccagtcctcg gcccagtagt ccaccgcgtc gatgaagccg ccggcccagg cctgcgcgat
     8281 gggcggcggc gtgccctcgg ccgtggccgc ggcgcgctcg tcctcgggca ggctggccag
     8341 caggccgcgc cagtcgatca cgcccggctc cagcgcctgc tcgtcgtcca gcgcctcgac
     8401 cggcgcctgc agctgagcgc gcagctgcgc ctcgcgcgcc agccagtgca tcatgaattg
     8461 ggtgcgctcg ccttcgctgg caaacacgtc gtcgccatcg aacagcaggg gcagccattc
     8521 ggtcggcggc acggtgcggc gcatgcacag cagcgccgtc agcacgccct cgcagtattc
     8581 ccactgcggc acgcgctggg ggtggtgctc gcgcagcgcg tccagggcgc cctccagcgc
     8641 ctccagcgta tcggcgcgcg ggtcggcggc gggctcggcg ggttcgggtg gcggggtaag
     8701 ggcttcggtc atggcgcaaa tcctttggct gcgccgatgg taacgacacg cggcgtcaac
     8761 gccgccacag ccccagccag accaccgcgc ccaccaccac cagcgcgccg gccacctgca
     8821 ccggcgccac cgcctgcccc agcaccagcc aggccagaat cagggcgaac accggctcca
     8881 cgttcatgat ggccgaatgg ccgaccaccc ccaggcgcgg cagcacggtg aaaagcaggg
     8941 tgatgccggt gccgtacagc agggtcagca gcgccagccc ccaccagccg ggcgtggctt
     9001 gcggcagcgc caggccgccg cgcgccagcg ccacggcgcc ggccagcagg ccgacgatgg
     9061 tcatggtggc cagggtgcgc aggcgcccgt ccagcgcgcc ggcctcgtgc tgcgtcagca
     9121 ccagggccag gccgaaggtg gccgccgccc ccagggcgaa tgccacgccg acgccgatct
     9181 gccgccactg cccggccgcg cccaggccgg aagcggcgcc cagcacgtcc agcgccagca
     9241 gcaggcccag cagcagcacc ggcatggcca ccagcaccgc acgctgcgga cggtgttgat
     9301 acagcaggcg cgcccacagc gcggtcatga gcggaaagct gttgaacacc agcagcgcca
     9361 gcgccaccgg cagccgcgcc acggccgaat acagccacag gctctgcacc gcgatcagcg
     9421 cgccgatcag cggcagcgcc ttgcgctggc gggcattgag cgccagcggc acctgctgcc
     9481 agtgcagcag caggcccacc accagcgccg tgcccaggct gcgcacggtg acggcggtgg
     9541 gcacgtccac gccgtgctgg aaggccagcc gcgccgacac gtggttggcc gccatcagca
     9601 gggcgatcag caacagggtg gcgaaaccgg ccgcggcacg cgccgacagg cgctcagaca
     9661 tacaggccgc gcacccgtgc gtgcagccgc gccaggccca gcagggcgtc ggtacagggc
     9721 gtggccacac cggtcagctg ccccagctcg cgcaccgcgc ccaccagggc gtcgatctcg
     9781 accgggcggc cggcctcgac gtcctgcagc atcgagggct tgaaggcgcc cagcttgcgc
     9841 gtgacctggt ggcggtcgtc gggcgtctgc tcgatcggca cgccgatgcg cgccccgatc
     9901 tcgcgcgcct ccagcatcac ggcgctgacg aagccgcgca ccagatcatc gtccaggatg
     9961 cggtcggcgc tggcgccggt aagggcgctg atggggttga tggtcatgtt gccccacagc
    10021 ttgtaccaga cgtccttctg gatctgcggc gagacggtga cctcgaaccg cgcctgccgc
    10081 agcagcgcgg ccagggcggc gaggcgcggc gtgtcgccgc ccccgggctc gcccacgatc
    10141 agaccgttgc cgaagtggcg gcggatcacc cccggcgcat cgaccgagca gctggcgtgc
    10201 accacggcac cgatcacgcg gcgcgcctcg atggcggcgg caatgctgcc acccgcatcg
    10261 accgactcca gccgcgtgcc cgccagcgcg ccgccaaagc cctgcagaaa ccaccagggc
    10321 acgccgttca tggccgtcag caccacggtg tcgggcccca gcagtggcgc gatgtgcgcc
    10381 gccacctgcc ccatggccgg cgccttgacg gcgacgatca ccagatcctg cacgcccagc
    10441 tcggccggct gctcgctggc ctgcacggcg tgcacggttt caatcactgc cccatcgtcc
    10501 cccgcctcgc gcaggcgcag cccatgcgcg cgcagcgccg ccagcgtggc gccgcgcgcc
    10561 acggcgctga cggccgcgcc ctgcgccgcc aggcgtgcgc ccaaccagcc gccgatggcg
    10621 ccggcaccgt agatgcagat tttgtgaaac attttggcct ccagcgcttg tccagcaagc
    10681 gccagcagat atcaaatcaa tagcaaataa agggcatgcg tcaggccacc tggccgtcgc
    10741 atgcacccca gcttcttcag tcagcgaagc gcagcgaggt catttgtcat tcgtcattct
    10801 tcaacccccc tcaagtcagc ccaacgcccc cagatgctgc tgcgcggcct gcgccagcgt
    10861 ttcgtcgaag gtggccagcg ggcagcgcag cgcgccggcc agccacaggt agctggcgtc
    10921 gtaggcgctg agctggtagc gctgggccag ggcaaaggtt tgcgccatat ccacttcgtg
    10981 cagttcgatg tccagtgtca gcgcatgcgc cagcccaggc agggcatgcg cttcctgacg
    11041 ccggtgcttc ttgagcgcga cgttgccgat ctcgtagatc agcagatggg gcgcatgcgg
    11101 tgtgctgcca tgcacctggt cgcgggcggc ctcgtaccag tgctcgcgaa acaccagccc
    11161 ggccagcacc gagcaatcga tcaccagggc cgggcgcgcc agatagggcg cgggcggctc
    11221 cgccacatga agctgcgggc tcaccggctg tcgcggtctt cgcggatcag ctgcacgctg
    11281 tcgctttggt cgaagatcgg ctcgggatac ttggtgcgca gctcgtcgta aatctgctcg
    11341 atcgtcttgt agccgcgccg cacgatgggc ccctgcgcgc tcatgccgac gatggtgccg
    11401 ccgcccaggg tgcgcgtgcc gatcagcccg gcgccgggct gatcgccctg ctgcacggcc
    11461 tgctcgatga tgctcatcag ctcgccctgc agcgagcggt ggttgcgcgc ggcgcgctgg
    11521 cgcagctgct gggccagggg ctcgggcacg tccttgatgg tcagggtggt catggcatga
    11581 tccagaatga atccacaatg gatgcatttt gcatgccacc gacgggcact gtcaaacggc
    11641 gcccagcgcc ctactggcgc cagctgccgt ccacgcgatc gacctgccgc accagggcgt
    11701 cgaacacgtc ctgcccggcg ccgcgcgcgg ggtcgaactg gaacgagtcg cgcgtgcagg
    11761 cggcggcaat gccctcgggc accggcagcg gcgcgcccat gctttgcgtg gccagctgga
    11821 tctcgcaggc gcgctgcagc gtccacagca cggcaaaggc cagcggcagc gtctcgcccc
    11881 aggacagcag gccgtggttg cgcaggatca cggctttacg ttggccgatg ctggccacca
    11941 cccgcggccc ctcgtcggcg cggatggtga tgccctcgaa gtcgtggtag gccaccatgt
    12001 cgtgcagttg cgcgctgtag aagttgcttt gcgacagccc gctggccagc gaagccaccg
    12061 ccaccccggc cgtggtgtgg gtgtgcatca cgcagtgcgc gccgggcacg ccgtcgtgga
    12121 tgcagctgtg cagcacgtag ccggcggggt tgatggggta gcgcgactcg ctcaggatgt
    12181 cgccggccag gttgaccttg accaggttgc tggccgtgac ctcgctgtag tgcaggccga
    12241 aggggttgat gagaaagtgc ttttcacccc cggtggcgct tgcgggcacg cgcagggtga
    12301 tgtggttgta gatcatttcg gtccagccga gcagggcgaa cacgcgatag caggcggcca
    12361 gttgcagacg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcggtcca
    12481 gccgagcagg gcgaacacgc gatagcaggc ggccagttgc agacgggcgg cgcgctcgtc
    12541 ggagtggcag tggtccagca cgctcatggt gcggccttgg gccaaacgcc gtcgaagtcg
    12601 ctggccgcat ggcgctcgcg caggccgcca ttgcggcggt tgaccaggcg gccgcgctgc
    12661 accgccgggc gggcggcgat ctgctgctgc cagcgcagca cgttcttgta cgtggccgcc
    12721 tccagaaact cggccgcgtt gtaggcctcg cccaccacca gcgcaccgta ccagggccag
    12781 atggccatgt cggcgatgct gtagtcgctg ccagccacgt attcgtgctc ggccagctgg
    12841 cggtccagca ggtcgagctg gcgcttgacc tccatggtga agcggtcgat ggggtactgc
    12901 tgcttgtcgt tggcgtaggc gtagaaatgc ccgaagccac cgcccaggta gggcgtggcg
    12961 cccatttgcc agaacagcca cgacaggcag gccgcgcgcg ccgccggcgc ggtggggatg
    13021 aaggcaccga atttttcggc caggtgcagc aggatggcgc ccgattcaaa aacggggatg
    13081 gcgggctgca cgctgcgatc gagcagcgcc ggaatcttgg aattggggtt gatcgcaaca
    13141 aagccgctgc cgaactgcgc gccctcgccg atgtcgatca gccaggcgtc gtactcggcg
    13201 ccggcgtggc cggcggccag cagctcttcc agcaggatgg tcaccttctg gccgttgggc
    13261 gtggccagcg agtacagctg cagcgggtgc tggccgaccg gcagctcctg ctcgaagcgc
    13321 gcgccggcgg tgggccggtt gatgttggcg aatttgttgt cttctttcgg ttcccagcgc
    13381 cagactttgg gcggaacgta tgcgctgctc atgcgcgtct ccttcacatt gacgacagct
    13441 cggccgcagg ccggcgcgac tcacccggcc gcaaacttct gcgccagggc gctggtttcg
    13501 cgggccagca cattggtgtc cagcccgacg gcgacgaagg tggctcccca ttccaggtag
    13561 cgctgggcgg tggcggcgtc gggcgagagg ttgccggccg ccttgccggc gcgcacgatg
    13621 cggcgcgtgg cgtcttccat gatggcctgc atctcggggt gggccgggtt gccgcggtgg
    13681 cccatgctgg ccgacaggtc tgccgggccg atgaagatgc cgtccacacc gtccaccgcg
    13741 gcaatcgctt ccaggttgtc cagcgcggtg cgcgtttcgg cctgcaccag gatgcagacg
    13801 cggtcattgg cctggtgcac gtacttcgga tagcggcccc agcgcgcggc gcgcccgccc
    13861 gccatgccgc ggatgccgcc cgcgccgccc ccgcccggat agcgcgcggc gcgcacgatg
    13921 gcggccgcct gctcgggcgt atcgaccatg ggcaccagca gggtctgcac gcccaggtcc
    13981 aggtactgct tgatcagcat ctcgcccaca tggccgtggc ccatgggcag gcgcgcgatg
    14041 gcgtgggtgc gcgggtaggc ggcgatggtc tgcagctggt gcaggatggt ttccaggctg
    14101 tgataggtgt gctcgccgtc gatcagcagc cagtcaaagc cggcgccggc ggcgatctcg
    14161 gccaccgtgg tgttgggcag attcagccag aaaccgatct ggcggcgttt ttcatgcagc
    14221 gcctgcttga aggggttgag cggtgtttcc atggtcagac aaacttgaac tgcaattgac
    14281 ccagcggccc gtagtcggcc tcgaacgcgt cgccgcgctt ggccgccacg gggcgggtga
    14341 aggagccggc cagcacgatc tgcccggcgc gcagcacctc gccccagggc gccagcttat
    14401 tggccagcca ggcgatgccg atggccgggt cgccctgcac gccggcggca agacccgttt
    14461 cctcgaccgc gccgttctgg cgcaggatgg cgccgaccca ggcacgatcg atggtgcgtg
    14521 cgtcggcgcg gccggcgccc accacgatgc cggcgttggc ggcgttgtcg ctgatggtgt
    14581 cgtagacctt gcgcatcacc ttggtgtggc ggtcgaactg ctcgatgcgg gcgtcgatga
    14641 tctcgatggc aggggtgacg taatcggtcg cagccagcac ctgctcgaca tcgacgtgcg
    14701 ggccagcgag gtcggccttg agcacgaagg ccagttccac ctcgacgcgc ggggcgatga
    14761 agcgatcggt ggggatgccg agcacctgcc ccggcgtgca ggtgtagagc atgctgtcca
    14821 gcagcgtgcc gtagtcgggc tcgtcgatct ggctggagat ctgcatggcg cgcgaggtca
    14881 ggccgatctt gtggccgatc acctgccgcc cctcggcccg ctgcagcgcc acccaggcgc
    14941 ggctgatgcg gtagccgtcc tcgatcgtca tgccgggaaa gcgcttcgag aagtgctcga
    15001 cctgctggcg cgatttttcg ctggcgtgca gttcggcggc gagctgctgg atctgttcgt
    15061 cggtcagcat gtcaggcctt gcgaaacagc gggtgcaggg tgctgtgttt ggcgtcgaac
    15121 acttcggggc cttcgtccac ctgcaccgtc aggccaatgt ggcgcgtggc catgcaaggg
    15181 tcgagccgcg cgcgcaacgc ccgcgcaatc gcctcgccgg cggcctgctg cgtggcgcta
    15241 ctgcgcccgc gcgccatgcg caggttggcg tacatgaagc cgtattcgcc cggatcgctg
    15301 cccgtgatgc ctgtcttgcc ggcaccggcc gcacgacccg ccgcgccgcc atcggccagc
    15361 gcgaaatgcg gcgccggata ggccagcacg cgcgtgccgc cggtgggaaa gacctgggcg
    15421 cccgcttcgt cgcgcaccgc cagcatggcg tcggcagcgt cgcggcacag ggcgggcatg
    15481 tcgacgatgg cgtcgaggtt gcccgagtaa aggatgacca gatggggcat ggcagcgttg
    15541 cgtcagatgc gcgcaaaggc gcgaaaaccg gtggcgcgcg aggcctcggg cggcggcacg
    15601 gcgtggccgt cctgcggggt gaccgggaac accgcgttca tctgcccggt gcccgaggcg
    15661 gcgaagtacg gcgtgatgac ttccatcttg ccgtcgtagg ccgaccagcc caaagcgccc
    15721 agcagcatgg cggtgtcgtg catcatgcct tcgccgtggc ccttggtggc gtattcgggc
    15781 agcatggcgc agaagtcctg ccactcgccg tttttccaca tctggatcac ctcgtgatcc
    15841 aggcgctcca gcaagggact ccagatcttg aaggcgaatt cgggcgccag gccgttttgc
    15901 gcgaagcggt gcgacagcga gccgctggcc atgaaggcga ccttgccgtc gtagtgcttt
    15961 tccaccgcct cgcgcatggc ccagcccagg cgcgcgctat cgtttaaata gtgactggtg
    16021 cacagcgccg acaccgacac caccttgaag tgctcgtccg cgttcatgta gcgcatgggc
    16081 accagggtgc cgtattcggg ctgcagcgaa gtggcgtcgt gcgccagcgt ctccacgccc
    16141 tgctcgttgc atacctgggc cagcagccgc cccagttccg ggttgccgtt gaagccgtag
    16201 gccatgttgc tgatgaagtg cggcagctcg ttgctggtgt agctgccctg ccagcgcggc
    16261 gcgcagttga ggtggtaggt ggcgttgacc agccagtggg tgtcgaacac cacgatggag
    16321 tccacgccca gctcgcggca gcggcggccg atttccttgt ggccgtcgat ggcgtcctgg
    16381 cgcgtgccct ggcgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcgc
    16501 tcaggtacat gctgggcacg tgggtgatct tggcggcaag ggcgagctgt cccatcactg
    16561 gtctccgagt tgcacggcgc gctgcggcgc cgtgtcgaca tgaagtgaaa acacgtccgg
    16621 ccgggcatag tggccgaccg ggtcgaaatc catctgcgcg gcggggatga tcgacaggtc
    16681 cagctcggcg gtgagcagcg cgtcctcgcc gaacaccggg ccggccagcc gctggcccag
    16741 tgggtcgtgg atgctgctgc cgccgcgcat cagcacggtg tcgggtgcct ccggcaggcg
    16801 gttgttcatg cgctcgcccg gaaagtccct gcgtcgcaga tgctggcagg ccgacagcac
    16861 gaagcagcgc ccttccaacg cgatgtgctg catggtcgat tgccaggaat cgcggtcgtc
    16921 ggcggttggc gcgcagtaga tcgccacgcg ctgctggtac atcgccagac gcagcgccgg
    16981 catgtagttt tcccagcaga tcaccgcgcc cagcttgccc cagggcgtgt ccaccgcctg
    17041 cagggtggag ccgtcgccaa agccccagac caggcgctcc agcgccgtcg gcatcagctt
    17101 gcggtgcttg ccgatgacgc gcccctgcgg ccccaggtag accgcggtgc agtacagcgt
    17161 gccgccttcg cgctcgatga tgccgaacac ggcgtacatc tggtggcgcg cgcaggcttc
    17221 ggccagttgc gccagttcgg gaccgtcgac gctgacggcg gcgtcgtagt agagcttgta
    17281 ctcggcgcgc ccctgcggcg tgcgcgcgcc caggtagatg tgaaagtccg ctcccttggg
    17341 gtagccgcca atgaaggctt cgggaaacac caccagctgc gcaccctggc gcgcagcctc
    17401 ggccatcagg cccagtgcgc gctcgacggt ggccggcgta tcgagcagca ccgaggaggc
    17461 ctgcaccacg gcggcggtca gcttgcccgc gctcatggcg cgctcctgcg ctgcgaggtg
    17521 acgatggggg tcatgcttgt ctccttttgt gtggtggggt gtgcgaggcg gcgggtcagc
    17581 cgtcggcgct gatgccggcg cgccggatca cctcggcgta gcgcgcggtc tcgctggcga
    17641 tgaaatcggc aaactgctgc ggcgtgccgg aacgggcggc gatgccaccc ttgtggaacg
    17701 cctcgacgac ggcgggctgc ttcatggcct cgttgacggc ggcgttcatg cgctgaacga
    17761 tggcgtccgg cacggcggcc ggcgccagca ggccattcca ggagttggat tcgatcttca
    17821 gcccctgctc ggccagcgtg gggatgtccg gcgcgtaggt cgagcgctgg gccgtggcca
    17881 tgccgatggc caccagcttg cccgactgca cgtggttgcg gaactccggc cagttgccga
    17941 acatcatgct cacctgcccg cccagcaggt cggccagcgc cggcgactga cccttgtagg
    18001 gcacgtgcac gatgtcaacg cccagctggg tcttgagcat ctcgcccgac aggtgggccg
    18061 aggtgccgtt gccgaacgag gcgtagctga gcttcttgcc cgccttggcc tgggccacga
    18121 tgtcggccac ggttttcagg ccgctgccgg ggtgggtggc cagcacatgc tcggacagcc
    18181 ccatcagcgc caccgggcgc agatccttca ggccgtcgta gggcagcttc ttcaccagcg
    18241 tctggttgac ggtgaagctg ttggccacgg tgacgaagct gctgccgtcg ggtgccgact
    18301 tggcgacgtt gtccacgccg atcacggtgc cggcgccggg cttgttctcg atcatcaccg
    18361 gctggccgag cgacctggcc agttcggtgg cgaccaggcg cgtcacgaaa tcggtggtac
    18421 cgccgggcgt gaagggcacg acgatgcgga tcggcttgct cggccacggg ctctgggcgc
    18481 gcgccatcga gggcgccagg gcgaccgccg ctgcggcctg gatgaggtat cgacgttgca
    18541 tgctttcaaa ctccccagtg cggaatgtga tgactgccca tcgacaccgc gatgttcttg
    18601 ggctccagaa aaacctcgta gctccaggtg ccgccttcgc gcccggtgcc gctggccttg
    18661 gtgccgccaa agggctggcg caggtcgcgc acgttctggc tgttgacgaa gcacatgccg
    18721 gcctcgatgg cggcggccac gcggtgggcg cggccgatgt tctcgctcca gacgtagctg
    18781 gacaggccgt actggatgtc gttggccttg gcgatcgcat cctgttcgtc cttgaacggg
    18841 atcaggcagg ccacggggcc gaagatctct tcctgcgcga tgcgcatgcg gttgtcgacg
    18901 tcggcaaaca cggtgggcca gacgaagttg ccgtccttca cgcgcgcgcc cagctcgggc
    18961 gcgtagctcg ggcgatccag gccgccgcac agcagcgtgg cgccctcctt gggccccagc
    19021 tcgatgtagc tgcgcacctt ggccaggtgg cccttgctga tcatggggcc gacgatggtt
    19081 gtctcgtcct gcgggtcgcc cacggtgatc ttcttcgcac gctcggcaaa cttctgcgca
    19141 aagtcggcgt agatgctttg ctgcaccagg atgcggctgc ccgccgtgca gcgctcgccg
    19201 ttgttggaga agatcatgaa cacggcggcg tcgagggcgc gatcgaggtc ggcatcgtcg
    19261 aagatcacga acgggctctt gccgcccagc tccatgctga acttcttcag gcccgcggcg
    19321 cgcacgatgc ggttgccggt ggccgtggag ccggtgaagc tgatggcgcg cacgtcgggg
    19381 tgggcgcaca gcggctcgcc ggcctccttg ccgaagccgt gcacgatgtt gagcacgccg
    19441 ggcgggatgc ccgcttccag cgccagctcg cccagccgcg cggcggtgag cggcgagagt
    19501 tcgctcatct tcagcaccgc ggtgttgccg aaggcgagac acggcgcgac cttccaggtc
    19561 gccgtcatga aaggcacgtt ccaggggctg atgagggcgc acacgcccac cgggtggaac
    19621 agcgtgtagt tcaggtgcgt gggcgtgggg taggtgtggc cgtcgacgcg cacgcacatc
    19681 tcggcaaagt agtcgaagtt gtcggccgcg cgcgggatca ggcccttgcc ggtctgggcg
    19741 atgacctggc cggtgtcgtc ggtctcggtg cgcgccagct ccggtacatg cttggcgatc
    19801 aaggcgccca gggcgcgcat cttcttcgcc cgctcgggcg cgggcaggcc ggcccacttg
    19861 gggaacgccg ccttggccgc ggccaccgcc gcgttgacct cggcctcgcc gccgctggcc
    19921 acctcggcca gcacgccctg cgtggcgggg ttggtggatt cgaagcagtc gctgctggca
    19981 accgctttgc cgtcgatgag gtgatcaatt ttcatagctg tttacgcttg actggcgggc
    20041 gctgcggccc gattggactc agaaaccatg gtggtctgca gcgcgcccag accttcgatc
    20101 tcgcacacca ctacggagcc gactgggcaa tcgaccacgc cgtcgggcgt gccggtcagg
    20161 atcagatcgc ccgggttcag cgtcatgaag ctgctgaagt actcgatcag cgtcggcacg
    20221 tcgaacacca tgtcggcggt cgtgccctgc tgggtcagct ggccgttgac gtgcgtgctc
    20281 agccgcaact tcatgggatc gggcacgtcg gccgcgtcga ccagccaggg gccgatgggg
    20341 gtgcaggtgt cgcggttctt gacgcgcagg ttgggccggt accagttttc caggtagtcg
    20401 cggatggcga aatcgttggc cacggtgtag cccgccacgt agtcgtaggc gtcgtccttg
    20461 cgcaccttct tcgcgctgcg gccaatcacc accgccagct cgcattcgta gtgcatgttc
    20521 tgcacgccgt cgggtcgcac cgtgtgggcg cgatggccga tcagcgaggc cgcgcccttg
    20581 gcaaaggcca gcggctcctc gggcggcttg aaggcgagtt cgcgcgcgtg gtcggcgtag
    20641 ttcaggccga gcgccagcac ggtgcgcgcg cgcggcacgg gcgccagcgg cggcagccag
    20701 accacctcgt caaaggacag gcggcggccg cggtacgcgg gcgtcagcag ttcgagctgg
    20761 ccttcggact cgacggcctc gtgcacggcg ccggcccagg cgatgcgggc gtgtttcatg
    20821 atgaacgtgc ccccacgctt ggcgctggcg cgccggcgct gccccccgag ggtgccctcg
    20881 cgccttgggg cggcccggcg gcgctcgact ccgcaacaac ggtatgcacc agcggcgcaa
    20941 agccgggcgc gctgacctcg acgcggtcgc ccgcctgcgc cagcggccgg ctgccatcgg
    21001 ccaggcagtc ggtgcccagc atcagcacgt cgccgggctg cagcgtcatg aaggcgctca
    21061 cgtcggccag cagggtggcg gcgtcgcgca ccagggtggc cagatccacg gtctgcacca
    21121 gcgcgccatt gacacgcgca tcgatctgca gcgcggcgat ctgcgccggc gccagcggcg
    21181 cggcgggtgt gccgcagacc agaaagccgt cgcggttgcg aaaacggatg gccgggcggt
    21241 agtagctgtc gtgcggcagg ctgaggtcgt tcagcagcac gcagccggcg atcgcgacac
    21301 cgccgagaac tatggttttg atagctgccg gcgcttgctg attgcgcgct gtaggccaat
    21361 tggatgcatg atctccgacc accaacccga gcgtcggccc cagccacacc ggctgcggca
    21421 cggcgaccgc ggcgccgcag gcggcaaggg tgttggcggt cttgacgtag agcaccgggg
    21481 ccttgggcgc cgcctggtac ggcgcctggc ccatgcgcgg cgcccacagc gcgtgttcgc
    21541 gtcgcaggtt cagcaacggg ccgtacacgg tgccggcggg ctgccagggc aggtcagggt
    21601 tcatcgttca tggctccatc agcgcttcga ccggctcggc gtcggcatgg gaaggcaggg
    21661 cgatcaactc gtccagcagg gtgtagagct ggttcaggcg cgcctgcccc aggtgcgccg
    21721 ccaggtcctg gtactgcgcc tcgatcgcgt cggacagcgc atcggccagc gccagcccgt
    21781 ccggcgtggc ggccaccagg ctgcggcgcg cgtcgtccgg acagcgttgg cgcgccacca
    21841 gaccgctctt ttccatgcgc gccagcatgc cggtcaggct ggggcccagg atgtaggcct
    21901 gctgcgccag ccgcccggtt tccaggccgc cctcgtgctc gcgcaacacg cgcagcacgc
    21961 gccattgctg gtccgacagg ccgtgcgccc gcaagccggg gcggtagtgc tgcatcagtg
    22021 cctcgcgcgc gcgcagcagc agcagcggca ggttgcggtg gacgaacggc gtcgggcggg
    22081 gcatttattt aatatattaa atttctgtcg gtggcgcaag cggttcacaa tctgcgcatg
    22141 accggacaaa ccctgagttc gcgcctcgcc acccgctggc ccctgctgca ggcgcccatg
    22201 gccggcgccc aggacagcgc catggcgctg gcggtgagcg gcgccggcgg cctgggcgcc
    22261 ctgccctgcg ccatgctggc gcccgacgcg ctgcggcgcg agctgcagac cctggccgcc
    22321 agcggcctgc cctacaacgt caactggttc tgccacccgc cgccggcgcc tgacgcggcg
    22381 cgcgaggcgg cctggcgcgc ggcgctcaaa ccctatttcg acgaactggg gctggacccg
    22441 gaagccgtgc ccggcggccc cggccgcgcg ccgttcagcc gggaggcggc cgagctgatg
    22501 gcggagttcc gcccgccggt ggtgagcttt cacttcgggc tgcccgcgcc cgagctgctg
    22561 acgcgcgtca aatcctgggg cgcttttgtg ctttcatcgg ccaccaccgt gcgcgaggcg
    22621 ctatggttac aggagcacgg tgccgatgcc atcatcgccc aggggctgga agccggcggc
    22681 catcgcggcc actttctgtc cgacgacctg agcgaacaga tgggcacgtt cgccctgctg
    22741 ccgcaggtgg tggccgccgt gcgcgtgccg gtgatcgccg ccggcggcat cgccagcgcc
    22801 gccggcgtgc gcgccgcgca ggcgctgggc gcggccggcg tgcaggtggg tacggcctac
    22861 ctgtgcgccg acgaggcgct caccacgccc gcgcaccgcg ccgcgctgca cagcgaggcg
    22921 gcgcgccaca ccgtcatcac caacctggtc accggccggc cggcgcgcgg catggtcaac
    22981 cgggtgatgc gcgagctggg gccgatcagc cccgcgccct cggcctttcc gctggccacg
    23041 gccgccatgg cctatctgcg cgccgccgcc gaaaagcagg gccggcacga cttcacccac
    23101 tggtggagcg gccagaacgc cagcggctgc cgggccgcgc cggcggccga catcacgcgc
    23161 gccctggcgc agggctttgc ctgaccccgc cacggccgtg gtgggcaccg ggcccgccct
    23221 cggcataacg catctcgtat gaccgatcgt ggacctgccg catagcctgc cggtgcgcgt
    23281 tcctttacga tgggaacgac ggcgccggtc ttgcgttggc gctttggact gcaacgccat
    23341 cgcctaccgc gccccacggg cgcacccctg aaggagcttg aacatgtctc gcatcgcgca
    23401 tctgctgagc ggcatcgcat tcgctgccac gacgctgggc ctgaccacac ccgcccaggc
    23461 gcagcaaacg atcgaatgcc gctccaacaa ctacggttac aacgagtgct atgcgggcga
    23521 cctgtcgtcc ccgcaactga tccaccagac ctccagcagc gcctgcatcg tcaaccgcag
    23581 ctgggggttc aacccgcgcc ggggctatat ctgggtcaat gaaggttgct ccggggtgtt
    23641 tgccgacgtc ggaggctacc accatggacg cggcgacacc tgggaccagg gggcgcgccg
    23701 ctacgacgac cgaggtcacg acgccggcgc catcgtcggc ggtctggtgt tgggcgccat
    23761 gctgggcgca gcggccggcg acgatcacga cgcgcaccgg catgcgcgcg accgccgctc
    23821 ggaagagatc gacacccgcc cgcagttcga ccgcgacggc aaccccaatt tcgacgtcca
    23881 cggcaactac cagggctgcc acggcctggg ctgtcaggtc gacaaccccg accgttgagc
    23941 gccgcccgaa cacccgccac agccacacct gcggagaaac cacatgaccc gaaccaaccg
    24001 agttcttttt cgcctggccg ccggactgac cacggcactg gcgctgcaag cagtgcacgc
    24061 ctcggacgac ccctgcttcg aaaaagccca gtcgcaggcc gcactgaacc agtgcgccag
    24121 cgccgcctac cagcgcgccg acgccgacct caaccggctc taccagctca tgacggcgcg
    24181 cctgagcacc gacgatgcgg cgcgcaagcg cctggtcgat gcgcagcgca agtggctgca
    24241 gtttcgcgat gccgagtgcg cgttccagac cgtacgcacg gtcggcggca gcgtccagcc
    24301 catgaatgtc agcagttgcc tgaccgatct gacgcgcgag cgggtgacgg atctgcaaaa
    24361 gcacctggac tgcgcgcgcg tcatgggtga aggcggcgcc tgcgccgtgc cgccgcggcg
    24421 ctgaagtcca cccccatcgc gggacggccc atccgggccg cccgccgttg cgcgcatgcc
    24481 gtcgaccgcc gtcctgagtc acgacatcta cccccacatc cgggtggtgc tgggcatggt
    24541 catcggcctg ggggtcacgc gcctgctgtc gggcctggcg cggctggtgc agcatccggg
    24601 ccagcaccgc atggatgccg tgcacctggg ctgggtcggc tccaccctgc tggcgctggt
    24661 gcacttctgg tggtggcagt tcgggctcta ccagatcgag gcctggacct ttggcaccta
    24721 cctgttcgtg atcggctacg ccatcgcgct gtttctgctg gcggcgctgc tgtttccgga
    24781 cagcatggcg ggctatgagg gctacggcga ctactttcag gagcgccgcg cctggttctt
    24841 tggcgtgctg gccagcacct atctgctgga cgtcatcgac acgctgctca agggcgacgc
    24901 gcacttcgcc cgctttgcgc gcgaatacct gatccgcacg ccgatcctgg tggctttgtg
    24961 catggttgcg gccagaaccg ctcggcggcg ctttcaggtc gccttcgtcg ctctggtgct
    25021 gatctatcag ctttcgtgga tcttccggct gttcaacaca ctgcagcaag cctgagaggc
    25081 gtcgcccgcc gtgccggcct cgccgcggcc atctgctccc tttctagcca tgatctcttt
    25141 cacccagcac cccatacccc tgcgctggcg ctggctgct
//
LOCUS       DAOQTZ010000034        24957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2332_length_24957_cov_14.531363, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000034 DAOQTZ010000000
VERSION     DAOQTZ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 24957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24957
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2332_length_24957_cov_14.531363"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /gene="paaA"
                     /locus_tag="PKN51_05860"
     CDS             <1..530
                     /gene="paaA"
                     /locus_tag="PKN51_05860"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HNT84660.1"
                     /translation="CRCSYGPYARAMVRVCKEESFHQRQGYEALLVMMKGTQAQRDMV
                     QDAVNRWWWKCLAMFGPPDADSPHSAQGMRWGIKRVSNDELRQKFIDATVPQAQVLGV
                     TLPDPELKWNEQRGHHDHGPIDWDEFWQTVGGHGPMNKERLATRVKAHDDGAWVREAA
                     LAHAAKQQQRARKAA"
     gene            547..846
                     /gene="paaB"
                     /locus_tag="PKN51_05865"
     CDS             547..846
                     /gene="paaB"
                     /locus_tag="PKN51_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HNT84661.1"
                     /translation="MSNAPALKDWPLWEVFVRSKQGLEHKHCGSLHASDAQHALQMAR
                     DVYTRRQEGVSIWVVPSHLIAASDPGEKDQLFEPASDKIYRHPTFYEIPEEVGHM"
     gene            846..1688
                     /gene="paaC"
                     /locus_tag="PKN51_05870"
     CDS             846..1688
                     /gene="paaC"
                     /locus_tag="PKN51_05870"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HNT84662.1"
                     /translation="MDARVSTRAPIDYLLHLADNALVLGQRNAEWCGHAPILEEDMAM
                     ANNSLDLIGQARLLYQYAAERLAGDVRAPRGAHWPAGPLTEDTLAYFRDVPEFRNYTL
                     LELPHSPPLVPTGVGDRDWGVTIVRNFLYSALMVLVWERLQRSADPQLAAIAAKSLKE
                     VRYHLRHARDWLVRLGDGTDASQRRAQAALDWLLPFTDEFWTASTHETEARDNASGVD
                     MAALRADWQALVNDALAEATLTRPAAQPGYVSQGKQGLHSEHLGYLLAEMQSLARAHP
                     GATW"
     gene            1706..2305
                     /gene="paaJ"
                     /locus_tag="PKN51_05875"
     CDS             1706..2305
                     /gene="paaJ"
                     /locus_tag="PKN51_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNT84663.1"
                     /translation="MSPIDLQRPTGEREQLSNQEQARLWALLDTLTDPEIPVVTLREM
                     GILRALRRGSDGAVEVVITPTYSGCPAMDQIADDIAATLRDAGVPGRVVTQLAPAWTT
                     DWMSEAARDKLRAYGIAPPRCASRRGGESVIHFAPKSRAAGARLAGADGPSDAEDAVA
                     CPQCGSTHTTETSHFGSTACKALYRCLDCLEPFDHFKSI"
     gene            2316..3419
                     /gene="paaK"
                     /locus_tag="PKN51_05880"
     CDS             2316..3419
                     /gene="paaK"
                     /locus_tag="PKN51_05880"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HNT84664.1"
                     /translation="MSAPRFHDLTVARINPEAAGAVAITFAVPPDLHGQFDFQPGQFL
                     TLRATIDGQDVRRNYSICSTRSRYARARELEVGIRPMEGGLFSNWAATQLRPGDTLAV
                     MPPDGRFTIRKPRALHRVGFAAGSGITPILSIIASTLEDSDSAKFTLVYGNRRMGSVM
                     FNEALQDLKDKYKDRLTLIHVLSRQAQEVPLLEGRIDAAKVQQLLDTLLPAKSMDEVF
                     ICGPEAMLEATESALRAAGVPAERIYTERFSSPRLDELSPEARRAAIAQPDPEAPAGQ
                     VALTVLIDGKPHELAMRRDQHVLDVALSAGLDAPYSCKAGVCCTCRAKVLEGSVSMDK
                     NFTLEEEEMAQGFVLSCQARPTSDRLVVSYDER"
     gene            complement(3475..3951)
                     /locus_tag="PKN51_05885"
     CDS             complement(3475..3951)
                     /locus_tag="PKN51_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNT84665.1"
                     /translation="MRCPNYTGFKPRVGGLAGARAPAGRAADDVAEGLALPERPPLGL
                     RGGRDLQRGMASWYGPGFHGRLTASGERFDQNDLTAAHKTLPFGTRVLVHSPRTGKQV
                     VVRINDRGPFVRGRVIDLSKAAARMLGLHALGHDAVVLRELTPDAGDQAAAADRAG"
     gene            complement(4260..5102)
                     /gene="metF"
                     /locus_tag="PKN51_05890"
     CDS             complement(4260..5102)
                     /gene="metF"
                     /locus_tag="PKN51_05890"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNT84666.1"
                     /translation="MSVNLSLEFFPPKTAEGADKLRAVRARLYALQPEFCSVTFGAGG
                     STQEGTLDTVRAIVAEGWPGVPHLSCIGQTAASIRERLASYRAAGVRRLVALRGDLPS
                     GYGMGGEFRHASDLVAFIRAETGDHFRIAVAAYPEFHPQARSPEADLDAFAAKVGAGA
                     DLAITQYFFNADAYFRFVDEARRRGVTVPIVPGIMPITNSTQLLRFSDSCGAEVPRWI
                     RLRLQGFGDDSAAIQDFGADVVAALCRRLHEGGAPGFHFYTLNQSAGVLELCRRGGWG
                     APFI"
     gene            complement(5099..5905)
                     /locus_tag="PKN51_05895"
     CDS             complement(5099..5905)
                     /locus_tag="PKN51_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNT84667.1"
                     /translation="MRADQRLVQAGLATSRAQAQRLIDAGARWRSAADAPWQPVRKSG
                     QELPPDAELQLLDAREAAYVSRGGLKLEGALRATGLRVAGWRCLDVGQSTGGFTDCLL
                     AHGAAQVTGVDVGHGQLHPRLRSDARVRVLEGVNARHLDASVLIAAGADQTGAEGQAG
                     THFDLIVGDLSFISLTLVLPALLPLLGSGGQLLLLVKPQFELQPADIGKGGIVRDPAL
                     YAQVEQRLRQACAALGLAVHHWLPSPITGGDGNREFFLHAARAATTCDTP"
     gene            complement(5905..6198)
                     /locus_tag="PKN51_05900"
     CDS             complement(5905..6198)
                     /locus_tag="PKN51_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84668.1"
                     /translation="MTRWLHAWGWVVLWLWIVGRSGRAFPIGLGSALVLAVLLVPGIC
                     LPLVLMRREDAPDLSGELSAPLAGLAVGIVGLHVLAAGSIALAEWRLWPQGDD"
     gene            complement(6214..7650)
                     /gene="ahcY"
                     /locus_tag="PKN51_05905"
     CDS             complement(6214..7650)
                     /gene="ahcY"
                     /locus_tag="PKN51_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNT84669.1"
                     /translation="MNAPLKNPVAQDSAIADITLADWGRKEIAIAETEMPGLMAIRAE
                     YAQHQPLKGARITGSLHMTIQTAVLIETLQALGAQVRWASCNIFSTQDHAAAAIAANG
                     TPVFAIKGESLADYWDYTHRIFEFGAAGSQAEGPNMILDDGGDATLLMHLGRQAESNP
                     GVLAAPKSEEERVLFAAIQARLKQDPSWYSRKCAEIIGVTEETTTGVHRLKEMSAAGT
                     LLFRAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIAVVCGYGDVGKGSAQ
                     ALRALSAQVWVTEIDPICALQAAMEGFRVVTMDEAAPLADIFVTATGNKDVITYAHMA
                     AMKDQAIVCNIGHFDNEIDVASLEARCQWEEIKPQVDHVIFEGGKRIILLAKGRLVNL
                     GCATGHPSYVMSSSFANQTIAQIELFTRPDAYAAGRVYVLPKHLDEKVARLQLAKLNA
                     RLTELSDEQARYIGVAKAGPYKPDSYRY"
     regulatory      complement(7717..7797)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            7903..9531
                     /locus_tag="PKN51_05910"
     CDS             7903..9531
                     /locus_tag="PKN51_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNT84670.1"
                     /translation="MSYVSETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGAVK
                     GRKASRHATSDWMEIEKQRGISVASSVMQMSYRDHVINLLDTPGHKDFSEDTYRVLTA
                     VDSALMVIDAANGVEAQTRRLIEVCRQRDTPIISFVNKMDREVRDPLDILDEVERELG
                     MPCCPMTWPVGQGKTFGGIIDLRTQSMTVFQAGSEKRPEDFEVIPLTEVDKLRARFGK
                     AYDDAIESMELAVGASIAWDHQEFLAARLTPVFFGSGVNNFGVQEVLNAVVDMSPPPG
                     PRMAWREVGREREQVTIRPEDKGFAGVVFKVQANMDANHRDRIAFVRVASGRYTPGMK
                     MKVQRTGKELRPTSVVTFMSQRREAVDEAYAGDIIGFTIHGGVQLGDSITDGPALQFT
                     GLPFFAPELFMTVVLKNPLRSKQLQQGLMELGEEGAIQVFKPDAGGNMLLGAVGQLQF
                     EVVQHRLKTEYDCDVRLEGCQYTGARWITADTPAELREFENAYPLRMAHDAAGTLTYL
                     CTSPYDVRLAQERFPKIHFHPLREHAGLELQARS"
     gene            9426..10931
                     /locus_tag="PKN51_05915"
     CDS             9426..10931
                     /locus_tag="PKN51_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84671.1"
                     /translation="MHQPVRRAAGAGAVSQDSLSPAEGACGVGTAGQVMSVTPQCPPD
                     AATRRRAVRIHLCTAAILLAVYLGWYAGVWPGPMGQDGYSLVANINQGGARYTDKDPA
                     WLLYALGTYGLSHRIEVLVLPLVLLQVLILTRLIGWLYVQRQPLAATGLLVLVACAPH
                     VLNFATSLYPDAIFSLAFVGLLFEIWLALRRGHLNGWTIAALFVLFPAACYFKGNGIV
                     MLVPVAYLAMRLKGGSRWLVVAIALSWTLAIQWGGKVADLGRGHGALKPLILFETVNF
                     MQTRPMNLWETRHMVTDRTREIMARHISQEDIDRLYDRDYWDTLWHQNQDRVRFRHMS
                     KTDFRHLRSDFLTYNLWRNLPAFTASRVNIFLASALAQGGMVGPDNARHGLRAVDTLS
                     VYNPFGFTRLPDTLNQLFHASYNWRFLLWSPFVGVALIGLCLRRSLRHRDPDALVISG
                     TLLVQLLGVFLFSIAAEYRYLLIFFYAPLLLLPMVLVRAAGQTPPRAQAAA"
     gene            10986..11333
                     /locus_tag="PKN51_05920"
     CDS             10986..11333
                     /locus_tag="PKN51_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84672.1"
                     /translation="MSSLTLLATLLCVFCIAVGQLLFKKAALALPPHPQLIDWLTNGW
                     LIASLALYGLTTLGWVWILRHAPLHLAYPFMGLAFLIVPTLAWAFLGEPLHWRTLAGG
                     VLIMAGVALASNS"
     gene            11344..12303
                     /locus_tag="PKN51_05925"
     CDS             11344..12303
                     /locus_tag="PKN51_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT84673.1"
                     /translation="MRIAVAIPCYKVTQHVLGVIAAIGPEVEAIYAVDDACPEGSGRF
                     IEQHNRDARVRVLYHPENRGVGGAVMTAYQAALADGVDIVVKIDGDGQMDPALIPKFV
                     RPILRGRADYTKGNRFYRPESLRGMPRIRLFGNAALSFVTKLSSGYWPIMDPTNGYTA
                     IHAAALRRLPLEKLERRYFFESDMLYRLNTIRAVVQDVPMDAVYADERSNLRVSSVLP
                     EFMRKHVQRFFKRYVYVYLVRDFNLGSVYSLAGVLLSVLGLGFGVAQWVHSVTTGRPA
                     SSGTVMLAALPLMIGIQFLIAFLHHDVDNVPREPLSADDRDPE"
     gene            12300..13133
                     /locus_tag="PKN51_05930"
     CDS             12300..13133
                     /locus_tag="PKN51_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNT84674.1"
                     /translation="MTPLSALPGTAWSAVHGVFTDIDDTLTTDGAITPDALQALGELK
                     AAGLRLIAITGRPVGWSEPFAATWPVDAIVAENGAVALIPDQIGLQRAPGVRRQLSKI
                     YQQDATLRAAQYQRMQQVLAHIEATVPGARRATDSPGRETDIAIDHSEFCHLPQAAIE
                     RCVQLMRAAGMNATVSSIHINGWFGPHNKLEGARWIVRRLLGRDLDAELARWIYVGDS
                     TNDQLMFEHFPHSVGVANIARFVPQLTHLPRYVTAAERGAGFAELAARLCAQRRGSPD
                     H"
     gene            13314..17315
                     /gene="putA"
                     /locus_tag="PKN51_05935"
     CDS             13314..17315
                     /gene="putA"
                     /locus_tag="PKN51_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HNT84675.1"
                     /translation="MSTTIGIKVDDLLRERIRNAAQNLNRTPHWLIKQAVVQYVDALE
                     RGATTIRVLGADEATPEDGREEAQPAAPQEAQPFLAFAQSILPQTPLRAAITAAWHRP
                     ETECLPALLPLARTQDEAQAAKVRELATRLVQGLRDAPVTSGVAALVQEFSLSSQEGV
                     ALMCLAEALLRIPDKATRDALIRDKISKGDWKSHVGRSPSLFVNAAAWGLVLTGKLTS
                     TNSEKSLSSALTRVIGKGGEPLIRQGVHRAMKLMGEQFVTGQNISEALANSRKYEAQG
                     FRYSYDMLGEAAATEADAQRYLRDYEQAIHAIGAASAGRGIFEGPGISIKLSALHPRY
                     SRAQYDRVMQELLPRVLHLAELAKQYDIGMNIDAEEADRLELSLDLMEALCAAPSLRG
                     WSGIGFVVQAYQKRCPHVIEYLIDLARRSRRRLMIRLVKGAYWDSEIKRAQLDGLAGY
                     PVYTRKVYTDVSYLACARKLLEAPDAVYPQFATHNAQTLASIYQLAASVGGSYYSGQY
                     EFQCLHGMGEPLYQQVTGPASEGKLARPCRIYAPVGSHETLLAYLVRRLLENGANTSF
                     VNRIGDASVPVAELVSDPVEDALAIAREEGRLGAPHPRIALPHDLFAGEGRLARANSQ
                     GLNLAHEQQLASLAAALLYSTRQTYLAAAPQTPLPANPAQTPGWEPLRNPAELSDIVG
                     WVREASADEAQAAAERAAQAAPIWAGTPPAARADALARAADLLEQRSQPLMGLIQREA
                     GKTLPNAVAEIREAVDFLRYYGAQVAAQFDNAAQRPLGVILAISPWNFPLAIFCGQVA
                     AALAAGNTVLAKPAEQTPLTAAAMVQILHEAGVPQGALQLVPGRGETVGAALVAHPQV
                     AGVMFTGSTEVARLIARQLSTRLSPNGHPIPLIAETGGQNAMIVDSSALAEQVVADVL
                     SSAFDSAGQRCSALRVLCLQEDVADRTLAMLHGALQEWTMGNPDRQSTDVGPVIDGEA
                     REQIEAHIKRMEDAGQKVTRVQRQDGQLHGHFVAPAIIEIDSTSRLTREVFGPVLHVI
                     RFQREQLDALIDGINATGYGLTFGVHSRIDETIAHLSERVQAGNLYVNRNVIGAVVGV
                     QPFGGMGLSGTGPKAGGPLYLHRLAQGAPNTALAALPRAEADQAQQPTLQLLARLQQT
                     TLPGVDAALARAACDAALAASRQGASWLLPGPTGESNRYRLLPRGPVWALPRTPLGLV
                     HQLAAALASGNPCHVVLPQGDQGCAAAWDAIAAALGAAGQDLLRRADAASLQDASTPV
                     TALLFEGDGDALLQVGALVAAREGAIVRVESRSSDELAAGLGYDLAALVHEQSISTNT
                     AAAGGNAQLMTMA"
     gene            complement(17349..17753)
                     /locus_tag="PKN51_05940"
     CDS             complement(17349..17753)
                     /locus_tag="PKN51_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84676.1"
                     /translation="MALAAEAAAASPQVVDTLGGRMHVRWDEGAAATPHGQLVFFAQF
                     LAATGVFERWVQGCPLAYHSGNAPDKRDVLGTLMLGLLIANIGAALLHIKAAAEQFKD
                     LDRWDCLLRYISDRIAPVIGPPKPPLRQPAPG"
     gene            17906..17998
                     /locus_tag="PKN51_05945"
                     /pseudo
     CDS             17906..17998
                     /locus_tag="PKN51_05945"
                     /inference="COORDINATES: protein motif:HMM:NF026200.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18167..19096)
                     /locus_tag="PKN51_05950"
     CDS             complement(18167..19096)
                     /locus_tag="PKN51_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT84677.1"
                     /translation="MPAYKPPPVSDRVHQFRIFMQILDTGSFVGAARALKIPPATVSV
                     AIRMLESELGVRLLHRTTRQVGLTHDGQRFLPMARMIVGDLDEICHLFTNDEEVVAGK
                     LHIRVPNRIVWRLIAPALPSFLQQHPELELRFSSSDRDPDPLGEDVDGVIRMGNVRDD
                     SLVHKPLGQLDRVNCASPRYLATHAALQHPDELVQHWAIGYLLPSEARRAVWHFTGAS
                     GAAQSIPVRHRVAVDDMDSYLACAQAGIGLIQAPRIDARHLLASGELVEVLPDWPATP
                     VAVTALYPHRHHQSRRLAAFLDWFGTLLDRAAA"
     gene            19112..19303
                     /locus_tag="PKN51_05955"
     CDS             19112..19303
                     /locus_tag="PKN51_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84678.1"
                     /translation="MNERSVCAESMILCFGFCMIPLFFLIVGMMGVKVRIRMARSHGR
                     SFGCGRLPFMGGQRRSKVC"
     gene            19350..20183
                     /locus_tag="PKN51_05960"
     CDS             19350..20183
                     /locus_tag="PKN51_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNT84679.1"
                     /translation="MEENQSQSAHLLIIDDDLDQLRLLVAALRGTSHRVSVATQGDQG
                     YVRAMVLQPNLILLDVCMPGRNGISVARLLKINPSTQHIPIIFLSALVGAQERLAGLK
                     AGAVDYITKPFLIEEVLERIRIHLELARKNLHPNTHDFTTKGSNQDPAVFNRQPVTLI
                     FRKLAIEYIMAHINESSLKVSDVAAHVEISVSKLNAIFEAIDGSSVFEFIRKERMQRS
                     ALMLGQSTLTIADIALEVGYSNPANFTTEFRKFWGRTPMQLRNESKINPDAWQQRIES
                     R"
     gene            20197..21879
                     /locus_tag="PKN51_05965"
     CDS             20197..21879
                     /locus_tag="PKN51_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT84680.1"
                     /translation="MDLNLATAAFITALVEFALGVSMLILWLREGRRYLAYWSSGFIA
                     FGFGSLLISMRNEIPDFLSIIVANFGTTLSSALLYVGICLFFGRRHSWLPWVVLALEA
                     AWLAYYTYVAYDTSARVYVYSVSQALIVLVTLQALFMVSRERAGRSNPEVVIVTVLLL
                     AVHLARIAGTPFFPLPRDFMNSGNFQTLLSFGLLMVHASYALALGNMHASGLNAELSA
                     ALAEAKIKERQKIEVLSYIGHDLRAPLATINGYSELLLADVSERGHRLLLSIQRIVKY
                     QVDLIDELLEYAKVELRPLAIRSTATDLPGLLRNISDYATTLCRQQNNQFIFQPSGRI
                     PRQIDIDGKRLQQVLLNLISNAAKFTHHGRVVLSIRAEPLKDISALYFSVSDTGVGID
                     LSRQTDIFSAFQDINAVKGGTGLGLFIAHRIVSAMGGSLRVASLPGQGSTFSFELSVP
                     VLEMSDFEWPAVAQQDGALVAPPTEFIFLEDALPDHQALDELADMASHGQFTDIECWI
                     ERHAQGAAHAAFAAHLYELLELFDFSAIRALALSCRNQKAAGRPNAQENSSK"
     gene            22333..23253
                     /locus_tag="PKN51_05970"
     CDS             22333..23253
                     /locus_tag="PKN51_05970"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84681.1"
                     /translation="MNTKSIVGRSFARHTLGWALLCAGFAAHAACTTSTPIFLSQGSG
                     GPTSLLSSAATLPVSFGSLGAAVRTYNAIGYNDVDDKLYGISNSGQILLEIDQTTGAS
                     TDVGAVSGLPAGSYNSGAFSNTGAYYVKPFSNNSQLYRIDVTASPPTATLITLSQAFT
                     TSDMAWVGSLMYSTADNGQLYSIDVATGTTTAIGSPDNTGGVLGAQFSGTNGLFGSAN
                     NGSGLYRINLTTGQRDKISDSPASGANDGASCPTVALFEPLPPEPPEPPEPPPPPTPV
                     PTLDFAGLGLLSLLGAGAGALGLRRRKRTH"
     gene            23360..24136
                     /locus_tag="PKN51_05975"
     CDS             23360..24136
                     /locus_tag="PKN51_05975"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84682.1"
                     /translation="MQVSRTLLAAAAVALFGLGPVAAQAAPPYVIVAGTGAVGADVGT
                     FPPGTSGTIAELGVDWQVTATAGATTGYHVYNGVQTWSFTRNGAPETVNLEFAISGID
                     AWLTTGGAVATEAIGLPVGTVCDISNPAYVFPAGALDGTPGGPVFSWTPDNGSGLAVL
                     QYLGNLGGVPVNRYLQYAPVPCTYTGSQLVLTGSGFPANEAGGTSFWHGLAYLRVMNQ
                     PVPPAAPTPVPTLDFAGLGLLSLLGAGAGALGLRRRKRSK"
     gene            complement(24254..>24957)
                     /locus_tag="PKN51_05980"
     CDS             complement(24254..>24957)
                     /locus_tag="PKN51_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNT84683.1"
                     /translation="RGYAPGRFSFNTGDGRCPACEGQGMRTLAMSFLPDVKLPCEVCH
                     GARFNPETLAVTWRGKSIGDVLRMEVDEAVGFFASMPAIAHPLQLLQDVGLGYLTLGQ
                     ASPTLSGGEAQRIKLVTELSKVRDDVTKRGRKAPHTLYVLDESTVGLHLQDVEKLIRV
                     LHRLVGGGHSVIVIEHDLDLIAEADWIIDLGPEGGDGGGRIVAVGTPEDVVRAGTHTG
                     VALGAVLGRTPMAPG"
BASE COUNT         4135 a         8517 c         8168 g         4137 t
ORIGIN      
        1 tgtgccgctg ctcctacggc ccctacgcgc gcgccatggt gcgcgtgtgc aaggaagaga
       61 gcttccacca gcgccagggc tacgaggccc tgctggtcat gatgaagggc acccaggcgc
      121 agcgcgacat ggtgcaggac gccgtcaacc gctggtggtg gaaatgcctg gccatgttcg
      181 gcccgcccga cgccgacagc ccgcacagcg cgcagggcat gcgctggggc atcaagcgcg
      241 tcagcaacga cgagctgcgc cagaagttca tcgacgccac cgtgccgcag gcgcaggtgc
      301 tgggcgtgac gctgcccgac cccgagctga agtggaacga acagcgcggc caccatgacc
      361 acggcccgat cgactgggac gagttctggc agaccgtggg cggccacggc cccatgaaca
      421 aggagcgcct ggccacccga gtgaaggcgc acgacgacgg tgcctgggtg cgcgaggccg
      481 cgctggccca tgccgccaag cagcaacaac gcgcccgcaa ggccgcctga tccccgagga
      541 gaaaccatga gcaacgcacc cgcactgaag gactggcccc tgtgggaggt cttcgtccgc
      601 agcaagcaag gcctggagca caagcactgc ggcagcctgc acgccagcga cgcgcagcac
      661 gcgctgcaga tggcgcgcga cgtctacacg cgcaggcagg aaggcgtgtc gatctgggtc
      721 gtgccctcgc acctgatcgc cgccagcgac ccgggcgaga aagaccagct gttcgagccg
      781 gccagcgaca agatctatcg ccacccgacc ttctacgaaa tccccgaaga agtggggcac
      841 atgtgatgga cgcccgcgtc agcacccgcg ccccgatcga ctacctgctg cacctggccg
      901 acaacgccct ggtgctgggc cagcgcaacg ccgaatggtg cggccacgcg cccatcctgg
      961 aagaggacat ggcgatggcc aacaacagcc tggacctgat cggccaggcg cgcctgctct
     1021 accagtacgc ggccgagcga ctggccggcg acgtgcgggc gccgcgcggc gcccactggc
     1081 cggccggtcc cctgaccgaa gacacgctgg cctacttccg cgacgtgccc gaattccgca
     1141 actacaccct gctggagctg ccacacagcc cgccgctggt gcccaccggc gtgggcgacc
     1201 gcgactgggg cgtcaccatc gtgcgcaact tcctctacag cgcgctgatg gtgctggtgt
     1261 gggagcgcct gcagcgctcg gccgaccccc agctggcggc catcgccgcc aagagcctga
     1321 aggaggtgcg ctaccacctg cgccacgcgc gcgactggct ggtgcgcctg ggcgacggca
     1381 ccgacgcctc gcagcgccgc gcgcaggccg cgctggactg gctgctgccc ttcaccgacg
     1441 agttctggac cgccagcacg cacgaaaccg aagcgcgcga caacgccagc ggcgtcgaca
     1501 tggcggcgct gcgcgccgac tggcaggcgc tggtcaacga cgcgctggcc gaggccacgc
     1561 tgacccgccc ggccgcgcag ccgggctacg tcagccaggg caagcaaggc ctgcattcgg
     1621 agcacctggg ctacctgctg gctgaaatgc agagcctggc gcgcgcccac ccgggcgcca
     1681 cctggtaagc cacgccggcc gccccatgag cccgatcgac ctccagcgcc caactggcga
     1741 gcgcgagcag ctatccaatc aggagcaagc cagactctgg gcgctgctcg acaccctgac
     1801 cgacccggag atcccggtgg tcacgctgcg cgagatgggc atcctgcgcg cgctgcggcg
     1861 gggcagcgac ggcgcggtgg aggtggtcat cacccccacc tacagcggct gcccggccat
     1921 ggaccagatc gccgacgaca tcgccgccac cttgcgcgac gccggcgtgc ccggccgtgt
     1981 ggtgacccag ctggcgccgg cctggaccac cgactggatg agcgaggccg cgcgcgacaa
     2041 gctgcgcgcc tacggcatcg cgccaccgcg ctgcgccagc cgccgcggcg gcgaatcggt
     2101 gatccatttt gctcctaaat caagagctgc cggcgctcgt ctggcgggcg ccgacggccc
     2161 atcggatgcc gaagatgcgg tggcctgccc gcaatgcggc tccacccaca ccaccgaaac
     2221 ctcgcatttc ggctccaccg cctgcaaggc gctgtaccgc tgcctcgact gcctggagcc
     2281 gttcgaccat ttcaagagca tctgaggaga cccgcgtgtc cgctccccgc tttcacgacc
     2341 tcaccgtcgc ccgcatcaac cccgaagccg ctggcgccgt ggccatcacc ttcgccgtgc
     2401 cgcccgacct gcacgggcag ttcgacttcc agcccggcca gtttctgacc ctgcgcgcca
     2461 ccatcgacgg ccaggacgtg cggcgcaact actcgatctg ctccacgcgc agccgctacg
     2521 cgcgcgcgcg cgagctggaa gtgggcatcc gaccgatgga aggcggcctg ttctccaact
     2581 gggccgccac ccagctgcgc ccgggcgaca ccctggccgt gatgccgccc gatggccgct
     2641 tcaccatccg caagccgcgc gccctgcacc gcgtgggttt tgccgccggc tcgggcatca
     2701 cacccatcct gtcgatcatc gccagcacac tggaagacag cgacagcgcc aagttcaccc
     2761 tggtctacgg caaccgccgc atgggcagcg tgatgttcaa cgaagcgctg caggacctga
     2821 aggacaagta caaggatcgc ctcaccctga tccacgtgct gtcgcgccag gcgcaggagg
     2881 tgccgctgct ggaaggccgc atcgacgccg ccaaggtgca gcagctgctc gacaccctgc
     2941 tgccggccaa gagcatggac gaggtcttca tctgcggccc cgaagccatg ctggaggcca
     3001 ccgaaagcgc gctgcgcgcc gccggcgtgc cggccgagcg catctacacc gagcgcttca
     3061 gctccccacg cctggacgag ctcagccccg aggcgcgccg cgccgccatc gcccagcccg
     3121 accccgaggc gccggccggc caggtggcgc tgacggtgct gatcgacggc aagccgcacg
     3181 agctggccat gcggcgcgac cagcatgtgc tggacgtggc cctcagcgcc gggctggacg
     3241 cgccctattc ctgcaaggcc ggcgtgtgct gcacctgtcg cgccaaggtg ctggaaggca
     3301 gcgtcagcat ggacaagaac ttcaccctgg aggaagagga aatggcccag ggcttcgtgc
     3361 tgagctgcca ggcccgcccc accagcgacc ggctggtggt cagctacgac gagcgctgag
     3421 cccgccgcaa tctgccacgg gcgctggtgc cggttgacct gatggcgacc gggatcagcc
     3481 ggcgcggtcg gcggcggcag cctggtcgcc cgcatcgggt gtcagctcgc gcagcaccac
     3541 ggcgtcgtga cccagggcgt gcaggcccag catgcgggcg gcggccttgc tcaggtcgat
     3601 cacccggccc ctgacgaacg gcccgcggtc gttgatgcgc accaccacct gcttgccggt
     3661 gcgcgggctg tgcaccagca cgcgggtgcc gaaaggcagg gtcttgtggg cggcggtgag
     3721 atcgttctgg tcgaagcgct cgccgctggc ggtcaggcgc ccgtgaaaac cggggccgta
     3781 ccacgaggcc atgccgcgtt gcaggtcgcg cccgccccgc agaccgagcg gcgggcgctc
     3841 gggcagggcc aggccttcgg ccacgtcatc ggcggcgcgg ccggccggcg cacgcgcgcc
     3901 cgccagcccg cccacgcgcg gcttgaagcc ggtgtagttg gggcagcgca tgtcgtgcac
     3961 cgccttggct gaagagcaag gctcggcaac ggcaggggtg gaagtgttca ggctcaccgt
     4021 ctgggccgcc agcggcccgg ctgccagcca cagggcgccg agacacagca gtcggcgggc
     4081 tggcggacgg tgggcacttc ccgaaaccgg cgtgccgcgc aagcgggcgc cgggagaggt
     4141 gtcccgtggc atggggccga ttttaacggc gccaccgccc cagccaggcg ctccggaccc
     4201 cccaatccag ggtttcagac ggtaaaaccc agataaaacc gttggcatta aaccaatggt
     4261 tatatgaaag gggcgcccca gcctcctcgg cggcacagtt ccagcacccc ggccgactgg
     4321 ttgagggtgt agaagtgaaa gcccggcgcg ccgccctcgt gcaggcggcg gcacagtgcg
     4381 gccaccacgt cggcgccgaa gtcctggatg gcggcgctgt cgtcgccgaa accctgcagc
     4441 cgcaggcgga tccagcgcgg cacctcggcg ccgcagctgt cggaaaagcg cagcaactgg
     4501 gtgctgttgg tgatcggcat gatgccgggc acgatcggca ccgtgacgcc gcgccggcgc
     4561 gcctcgtcga cgaagcgaaa gtaggcgtcg gcgttgaaga agtattgcgt gatggccagg
     4621 tccgccccgg cgcccacctt ggcggcgaag gcgtccaggt cggcctcggg cgagcgcgcc
     4681 tgcgggtgga actcagggta ggcggccacc gcgatgcgga agtgatcgcc ggtttcggca
     4741 cggatgaagg ccaccaggtc gctggcgtgg cggaattcgc cgcccatgcc gtagccgctg
     4801 ggcagatcgc cgcgcagggc caccagtcgc cgcaccccgg cggcgcggta gctggccagg
     4861 cgctcgcgga tgctggcggc ggtctggccg atgcacgaca ggtggggcac gccgggccag
     4921 ccttcggcga cgatggcgcg caccgtgtcc agcgtgcctt cctgcgtcga gccgccggcg
     4981 ccgaaggtga cggagcagaa ctcgggttgc agcgcgtaca gccgggcgcg cacggcacgc
     5041 agcttgtcgg cgccctcggc cgtcttgggg gggaagaatt cgagactgag gttgacgctc
     5101 atggggtatc gcaagtcgtc gccgcgcgcg cggcatggag aaagaattcc cggttgccgt
     5161 cgccaccggt gatggggctg ggcaaccagt ggtgcacggc caggccgagc gcggcgcagg
     5221 cctggcgcag ccgctgctcg acctgcgcgt acagggccgg atcgcgcacg atgccgccct
     5281 tgccgatgtc ggccggctgc agctcgaact gcggcttgac cagcagcagc aactgcccgc
     5341 cgctgcccag cagcggcagc agcgccggca gcaccagggt cagcgaaatg aaggacagat
     5401 cgcccacgat caggtcgaaa tgagtgccag cttggccttc agcgcccgtc tggtcagcgc
     5461 cagcagctat caaaacagaa gcatccaggt gccgggcgtt gacaccttcg agcacccgca
     5521 cgcgggcgtc gctgcgcagg cgcggatgca gctggccgtg gcccacgtcg accccggtca
     5581 cctgcgccgc accgtgcgcc agcaggcagt cggtgaagcc gccggtggac tgcccgacgt
     5641 ccaggcagcg ccagccggcc acgcgcagcc cggtggcgcg cagcgcgccc tccagcttga
     5701 ggccaccgcg cgagacgtac gccgcctcgc gcgcatccag cagttgcagc tcggcgtcgg
     5761 gcggcagctc ctggccgctc ttgcgcaccg gctgccaggg cgcgtcggcg gcgctgcgcc
     5821 agcgcgcacc ggcgtcgatc aggcgctgcg cctgagcgcg cgaggtggcc agcccggcct
     5881 gcaccaggcg ctggtcggcg cgcatcagtc gtcgccctgc ggccacagcc gccattcagc
     5941 cagcgcgatg ctgcccgcgg ccagcacgtg caggccgaca atgcccaccg ccagacccgc
     6001 cagcggcgcc gacagctcgc ccgacaggtc cggcgcgtcc tcgcgccgca tcagcaccag
     6061 cggcagacag atgcccggca ccagcagcac cgccagaacc agggcgctgc ccaggccgat
     6121 cggaaaggcg cgaccgctgc gtccgacgat ccacagccac agcaccaccc agccccaggc
     6181 atgcagccag cgcgtcatgg ggtccgcccg ggatcagtag cggtagctgt cgggcttgta
     6241 cggcccggcc ttggccacgc cgatgtagcg cgcctgctcg tcgctcagct cggtcaggcg
     6301 cgcgttcagc ttggccagct gcaggcgcgc gactttttcg tccaagtgct tgggcagcac
     6361 gtagacgcgg ccagcggcgt aggcgtcggg gcgggtgaac agctcgatct gggcgatggt
     6421 ctggttggcg aagctggagc tcatcacgta gctggggtgg ccggtggcgc agcccaggtt
     6481 caccaggcgc cccttggcca gcaggatgat gcgcttgccg ccttcgaaga tgacgtggtc
     6541 gacctgcggc ttgatctcct cccactggca gcgcgcttcc aggctggcga cgtcgatctc
     6601 gttgtcgaag tggccgatgt tgcagacgat ggcctggtcc ttcatggccg ccatgtgggc
     6661 gtaggtgatg acgtccttgt tgccggtggc ggtgacgaag atgtcggcca gcggcgccgc
     6721 ctcgtccatg gtgaccacgc gaaagccttc catggccgcc tgcagcgcgc agatggggtc
     6781 gatctcggtc acccacacct gggccgacag cgcgcgcagc gcctgcgcgc tgcccttgcc
     6841 gacgtcgccg tagccgcaca ccacggcgat cttgccggcc accatgacgt cggtggcgcg
     6901 cttgatgcca tccaccagcg actcgcggca gccgtacagg ttgtcgaact tgctcttggt
     6961 gaccgagtcg ttgacgttga tggcgcgaaa cagcagcgtg ccggcggcgc tcatctcctt
     7021 caggcggtgc acgccggtgg tggtttcctc ggtcacgccg atgatctcgg cgcacttgcg
     7081 gctgtaccag ctcgggtcct gcttgagccg ggcctgaatg gcggcaaaca gcacccgctc
     7141 ctcctcgctc ttgggggcgg ccagcacgcc ggggttggac tcggcctgac ggcccagatg
     7201 catcagcagc gtggcgtcgc cgccgtcgtc caggatcatg ttggggcctt cggcctggct
     7261 gcccgcagcg ccgaattcga agatgcggtg cgtgtagtcc cagtagtcgg ccaggctttc
     7321 tcccttgatg gcgaacaccg gcgtgccgtt ggcggcgatg gcggcggcgg cgtggtcctg
     7381 ggtcgagaag atgttgcacg aggcccagcg cacctgcgcc cccaacgcct gcagggtctc
     7441 gatcagcacg gcggtctgaa tcgtcatgtg caggctgccg gtgatgcgcg cgcccttgag
     7501 tggctggtgc tgggcgtact cggcgcggat cgccatcagg ccgggcatct cggtctcggc
     7561 gatggcgatt tccttgcggc cccagtcggc cagggtgatg tcggcgatgg cggagtcctg
     7621 cgccacgggg ttcttcaggg gtgcgttcat ggtttgctcc aaaggcaatg cttttgaaaa
     7681 accactgacg ggatgagcag ctcaccacac gcgatcagtg ggtgagcgtc gttgccttga
     7741 tcgaattccg agcctcgcac cccgctggcc gggggtgctg caacgctcct cggaagtggg
     7801 cggcattata gcggcgctcc ctcagggacg aagggcaacg ataattgacg gttttttggc
     7861 gcgccgcatg ccccgatcgg ctcgcgcccc ggagtcgtca tcttgtctta cgtttccgaa
     7921 acccggcgcc gccgcacctt cgccatcatt tcccaccccg acgcgggcaa gaccacgctg
     7981 acggaaaagc tgctgctgtt ctccggcgcg atccagatcg caggcgccgt caagggccgc
     8041 aaggccagcc gccatgccac gagcgactgg atggagatcg agaagcagcg cggcatctcg
     8101 gtggcctcca gcgtgatgca gatgagctac cgcgaccatg tcatcaacct gctcgacacg
     8161 cccggccaca aggacttctc cgaggacacc taccgcgtgc tcaccgccgt cgattcggcg
     8221 ctgatggtga tcgacgcggc caacggcgtg gaggcgcaga cgaggcgcct gatcgaggtc
     8281 tgccgccagc gggacacacc catcatcagc ttcgtcaaca agatggaccg cgaggtgcgc
     8341 gacccgctgg acatcctcga cgaggtcgag cgcgagctcg gcatgccctg ctgccccatg
     8401 acctggcccg tgggccaggg caagaccttt ggcggcatca tcgacctgcg cacgcagagc
     8461 atgacggtgt tccaggccgg cagcgaaaag cgcccggagg acttcgaggt catcccgctc
     8521 accgaggtgg acaagctgcg cgcacgcttc ggcaaggcct acgacgacgc catcgagagc
     8581 atggagctgg ccgtgggcgc gtcgattgcc tgggaccacc aggagtttct ggccgcgcgc
     8641 ctgaccccgg tgttcttcgg ctcgggcgtg aacaacttcg gtgtgcagga ggtgctcaac
     8701 gccgtggtcg acatgtcgcc cccgcccggc ccgcgcatgg cctggcgcga agtgggccgc
     8761 gagcgcgagc aggtcaccat ccgccccgag gacaagggct ttgccggcgt ggtcttcaag
     8821 gtgcaggcca acatggacgc caaccaccgc gaccgcatcg ccttcgtgcg cgtggcctct
     8881 ggccgctaca cgccaggcat gaagatgaag gtgcagcgca cgggcaagga actgcgcccg
     8941 accagcgtcg tcaccttcat gagccagcgg cgcgaggcgg tcgacgaagc ctacgcgggc
     9001 gacatcatcg gcttcaccat ccacggcggc gtgcagctgg gcgactccat caccgacggc
     9061 ccggcgctgc agttcaccgg cctgccgttc ttcgcgcccg agctgttcat gaccgtggtg
     9121 ctcaagaacc cgctgcgctc caagcagctg cagcagggcc tgatggagct gggcgaggaa
     9181 ggcgccatcc aggtcttcaa gcccgacgcc ggcggcaaca tgctgctggg cgccgtgggc
     9241 cagctgcagt tcgaggtggt gcaacaccgc ctgaagaccg aatacgactg cgacgtgcgc
     9301 ctcgaaggct gccagtacac gggcgcgcgc tggatcacgg ccgacacgcc ggccgagctg
     9361 cgcgaattcg agaacgccta cccgctgcgc atggcgcacg atgcggcggg cacactgact
     9421 tatctgtgca ccagcccgta cgacgtgcgg ctggcgcagg agcggtttcc caagattcac
     9481 tttcacccgc tgagggagca tgcggggttg gaactgcagg ccaggtcatg agcgtcacgc
     9541 cccagtgccc acccgacgct gccacgcggc ggcgcgcggt gcggatccat ctctgcaccg
     9601 ccgcgatcct gctggcggtc tacctggggt ggtatgcggg tgtctggcct ggacccatgg
     9661 gtcaggatgg ttactccctg gtggccaaca tcaaccaggg cggcgcccga tacaccgaca
     9721 aagatccggc ctggctgctg tatgcgctgg gcacctacgg cctgagccac cggatcgagg
     9781 tgctggtgct gcctctggtg ttgctgcagg tgctcattct gacccgcctc atcggctggc
     9841 tctacgttca gcgacagccg ctggcggcca cggggctgct ggtgctggtc gcctgcgcgc
     9901 cacatgtgct caactttgcc accagcctgt atccggatgc cattttcagc ctggccttcg
     9961 tggggctgct gtttgaaatc tggttggcgt tgcgtcgcgg ccacctcaac ggctggacca
    10021 tcgcggcctt gttcgtgctg ttcccggcgg cctgctactt caagggcaac gggatcgtca
    10081 tgctggtgcc ggtggcgtac ctggccatgc ggctcaaagg cggcagccgc tggctggtcg
    10141 tggccattgc gctgtcctgg accctggcca tccaatgggg cggcaaggtg gcggacctgg
    10201 ggcgcggcca cggcgcgctc aaaccgctca tcctgttcga gacggtgaac ttcatgcaga
    10261 cgcggcccat gaacctgtgg gagacccggc acatggtcac ggatcgcacc agggagatca
    10321 tggcccgcca catcagccag gaagacatcg atcgcctcta cgaccgcgac tactgggaca
    10381 cgctgtggca ccagaaccag gatcgcgtcc gcttccgcca catgagcaag acggatttcc
    10441 gccacctgcg ctcggatttc ctgacctaca acctctggcg caacctgcct gcgttcacgg
    10501 ccagccgcgt caacattttt ctggcctcgg ccctggctca gggaggcatg gtgggcccgg
    10561 acaacgccag acacggcctg cgcgcggtgg acaccctctc cgtctacaac ccgttcgggt
    10621 tcacacgact gccggacacg ctgaaccagc tgttccatgc cagctacaac tggcgctttc
    10681 tgctctggag tccgttcgtc ggtgtggcgc tcatcgggct gtgtttgcga cgttcgctgc
    10741 gccatcggga cccggacgcg ctggtgatca gcggcacgct gctggtgcag ctgctggggg
    10801 tgttcctgtt ttcgatcgcc gccgagtacc gctacctgct gatcttcttc tatgcaccgc
    10861 tgctgctgtt gccgatggtt ctggtgcgcg cagcaggtca gacgccgcca cgggcgcagg
    10921 ccgccgcctg agtttggtgc gggcgggcct gcaatgctag attcacccta ctttccaagg
    10981 cattcatgtc cagtctgacc ctcctggcca ccctgctgtg cgtgttctgc atcgccgtcg
    11041 ggcagctgct gttcaaaaag gccgcgctgg cgctgccgcc gcacccgcag ctcatcgact
    11101 ggctaaccaa tggctggctg atcgcctcgc tggcgctcta tgggctgacc accctgggct
    11161 gggtctggat cctgcgccat gcgccgctgc acctggccta ccccttcatg ggcctggcct
    11221 tcctgatcgt gcccaccctg gcctgggcgt tcctgggcga gccgctgcac tggcgcacgc
    11281 tggccggcgg cgtgctgatc atggccggcg tggcgctggc gtccaactcc tgaggccggc
    11341 ccgatgcgca tcgccgtcgc cattccctgc tacaaggtca cgcagcacgt gctgggcgtg
    11401 atcgcggcca tcgggcccga ggtcgaggcc atctacgcgg tggacgacgc ctgccccgag
    11461 ggcagcggcc gcttcatcga gcagcacaac cgcgatgcgc gcgtgcgcgt gctctaccac
    11521 cccgagaacc gcggcgtggg cggtgccgtg atgaccgcct accaggcggc actggcggac
    11581 ggtgtggaca tcgtggtcaa gatcgacggc gacggccaga tggatccggc gctgatcccc
    11641 aagttcgtgc gccccatcct gcgcgggcgc gccgactaca ccaagggcaa ccgcttctac
    11701 cgccccgagt cgctgcgcgg catgccgcgc atccggttgt tcggcaacgc ggcgctgtcg
    11761 ttcgtcacca agctcagttc gggctactgg ccgatcatgg acccgaccaa cggctacacc
    11821 gccatccatg ccgcggcgct gcgtcggctg cccctcgaaa agctggagcg tcgctacttt
    11881 ttcgaaagcg acatgctgta ccgcctgaac accatccgcg ccgtggtgca ggacgtgccg
    11941 atggacgccg tgtacgccga cgagcggtcc aacctgcggg tgtcgagcgt gctgcccgag
    12001 ttcatgcgca agcatgtgca gcgcttcttc aagcgctatg tctacgtcta cctggtgcgc
    12061 gacttcaacc tgggcagcgt ctacagcctg gccggcgtgc tgctgagtgt gctggggctg
    12121 ggcttcgggg tggcgcagtg ggtgcacagc gtgaccacgg gccggccggc gtcgagcggc
    12181 acggtgatgc tggcggcgct gccgctgatg atcggtatcc agttcctgat cgcctttctg
    12241 caccatgacg tcgacaacgt gccgcgcgag ccgctgtcgg ccgacgaccg ggatcccgag
    12301 tgacgccgct gtccgccctg ccgggcacgg cgtggtccgc ggtgcacggg gtgttcaccg
    12361 acatcgacga cacgctgacc accgacggcg ccatcacgcc cgacgcgctg caggcgctgg
    12421 gcgagctgaa ggcggccggc ctgcggctga tcgccatcac cggccgcccg gtgggctgga
    12481 gcgaaccctt cgccgccacc tggccggtcg atgccatcgt ggccgagaac ggggcggtgg
    12541 cactgattcc agatcaaatc ggcctccagc gcgcgcccgg cgtgcgccga cagctatcaa
    12601 aaatatacca acaggacgcc accctgcgtg ccgcgcagta ccagcgcatg caacaggtgc
    12661 tggcccacat cgaggccacg gtgcccggcg cgcggcgcgc caccgattcg cccgggcgcg
    12721 agaccgacat cgccatcgac cacagcgagt tctgccacct gccgcaggcg gcgatcgagc
    12781 gctgcgtgca gctcatgcgc gccgccggca tgaacgccac cgtcagcagc atccacatca
    12841 acggctggtt cggcccgcac aacaagctgg agggcgcgcg ctggatcgtg cgccgcttgc
    12901 tggggcgcga tctggacgcc gagctggcgc gctggatcta tgtgggtgat tcgaccaacg
    12961 accagctcat gttcgagcat ttcccacaca gcgtgggggt ggccaacatc gcgcgcttcg
    13021 tgccccagct gacccacttg ccgcgctacg tgacggcggc cgagcgcggc gccggctttg
    13081 ccgagctggc ggcgcgcctg tgcgcgcagc ggcgcgggtc acccgaccac tgacaaggct
    13141 ttcgggcgat ttgggggctg ccccaccgac ctcgccggcc ggacaggcac gatccgaatt
    13201 gtggttgcac caagggttaa ccctgatatc cgacaggtgc aacctatgga acagttccgg
    13261 ctgcaccgga ccaggcacac ccggccaccc tctccaccgc ccccgagaaa cccatgtcta
    13321 ccaccattgg catcaaggtc gatgacctgc tgcgcgagcg cattcgcaac gccgcgcaga
    13381 acctcaaccg cacgccgcac tggctgatca agcaggccgt cgtccaatat gtggacgcgc
    13441 tcgagcgtgg cgccaccacc atccgcgtcc tcggcgccga cgaggccacg cccgaggacg
    13501 gccgggagga ggcgcaaccc gccgccccgc aggaggcgca acccttcctg gccttcgccc
    13561 agtccatcct gccgcagacg ccgctgcgcg ccgccatcac ggccgcctgg catcggcccg
    13621 agaccgaatg cctgcccgcg ctgctgccgc tggcacgcac ccaggacgag gcccaggccg
    13681 ccaaggtgcg cgagctcgcc acgcgcctgg tgcagggcct gcgcgatgcg cccgtcacca
    13741 gcggcgtggc cgcgctggtg caggagttct cgctctccag ccaggagggc gtggcgctga
    13801 tgtgcctggc cgaggcgctg ctgcgcatcc ccgacaaggc cacgcgcgac gcgctgattc
    13861 gcgacaagat cagcaagggc gactggaagt cgcatgtggg ccgctcgccg tcgctcttcg
    13921 tcaacgccgc cgcctggggc ctggtgctga cgggcaagct gacttccacc aacagcgaga
    13981 agagcctgtc gtctgcgctc acgcgcgtca tcggcaaggg cggcgagccg ctgatccgcc
    14041 agggcgtgca ccgcgccatg aagctcatgg gcgagcagtt cgtcaccggc cagaacatct
    14101 ccgaggccct ggccaacagc cgcaagtacg aggcccaggg ctttcgctac agctacgaca
    14161 tgctgggcga ggccgccgcg acggaagccg acgcccagcg ctacctgcgc gactacgagc
    14221 aggccatcca cgccatcggt gcggcatccg ccgggcgcgg catcttcgag ggcccgggca
    14281 tctccatcaa gctctcggcc ctgcacccgc gctattcgcg cgcgcagtac gaccgcgtga
    14341 tgcaggagct gctgccgcgc gtgctgcacc tggcggaact ggccaagcag tacgacatcg
    14401 gcatgaacat cgacgccgag gaggccgacc gcctggagct gtcgcttgac ctgatggagg
    14461 cgctgtgcgc cgcgccttcg ctccgcggct ggagcggcat agggtttgtg gtgcaggcgt
    14521 atcaaaagcg ctgcccgcat gtcatcgaat acctgatcga cctggcgcgg cgcagccgcc
    14581 gccgcctcat gatccgcctg gtcaagggcg cgtactggga cagcgagatc aagcgcgccc
    14641 agctcgatgg cctggccggc tacccggtct acacgcgcaa ggtctatacc gacgtgagct
    14701 acctggcctg cgcgagaaag ctcctggaag cacccgacgc cgtctacccg cagttcgcca
    14761 cacacaacgc ccagacgctg gccagcatct accagctggc cgccagcgtg ggcggcagct
    14821 actacagcgg ccagtatgag tttcaatgcc tgcacggcat gggcgagccg ctgtaccagc
    14881 aggtcaccgg ccccgcatcg gaaggcaagc tcgcgcgccc ctgccgcatc tacgcccccg
    14941 tgggcagcca cgagacgctg ctcgcctacc tggtgcggcg cctcttggaa aacggcgcca
    15001 acacctcgtt cgtcaaccgc ataggcgacg ccagcgtgcc cgtggccgag ctcgtgagcg
    15061 acccggtcga ggacgcgctc gccatcgccc gcgaggaggg ccgcctgggc gcaccgcatc
    15121 cgcgcattgc cctgccccac gacctgttcg cgggcgaggg ccgtctggcg cgcgccaact
    15181 cccagggcct gaacctggcg cacgagcagc agctcgcatc gctggccgcc gccctgctct
    15241 acagcacgcg ccagacctat ctggccgcgg cgccccagac cccgctgccc gccaacccgg
    15301 cgcagacgcc gggctgggag ccgctgcgca acccggccga gctttccgac atcgtgggct
    15361 gggtgcgcga ggccagtgcc gacgaggccc aggccgccgc cgagcgcgcc gcccaggccg
    15421 cgcccatctg ggccggcacg cccccggccg cacgcgccga tgccctggcc cgcgcggccg
    15481 acctgctcga gcagcgcagc cagccgctca tgggcctgat ccagcgcgag gccggcaaga
    15541 ccctgcccaa cgccgtggcc gagatccgcg aggccgtgga cttcctgcgc tactacggcg
    15601 cccaggtggc ggcgcagttc gacaacgccg cacagcgccc gctcggcgtc atcctcgcca
    15661 tcagcccctg gaacttcccg ctggccatct tctgcggcca ggtggccgcg gccctggccg
    15721 cgggcaacac cgttctggcc aagccggccg agcagacgcc gctcacggcc gcggccatgg
    15781 tgcagatcct gcacgaggcc ggcgtgcccc agggcgcgct gcagctcgtg cccggccgcg
    15841 gcgagaccgt gggcgcggcc ctggtggcgc acccgcaggt cgcgggcgtg atgttcaccg
    15901 gctccaccga ggtggcgcgc ctgatcgcgc gccagctcag cacccgcctg tcgcccaacg
    15961 gccaccccat cccgctgatc gccgagaccg gcgggcagaa cgccatgatc gtggactcgt
    16021 cagccctggc cgagcaggtg gtggccgacg tgctgtcctc ggccttcgac tcggccggtc
    16081 agcgctgctc ggcgctgcgc gtgctatgcc tgcaggagga cgtggccgat cgcaccttgg
    16141 ccatgctgca cggcgcgctg caggaatgga ccatgggcaa ccccgaccgc cagagcaccg
    16201 acgtgggccc cgtgatcgac ggcgaggcgc gcgagcagat cgaggcgcat atcaagcgca
    16261 tggaggacgc gggccagaag gtcacgcgcg tgcagcgcca ggatggccag ctccacggcc
    16321 atttcgtggc tccggccatc attgaaattg atagcacatc gcgcttgacc cgtgaggtgt
    16381 ttggcccggt cttgcatgtc atccgattcc agcgcgagca gctcgacgcc ctgatcgacg
    16441 gcatcaacgc cacgggctat ggcctgactt ttggcgtgca cagccgcatc gacgagacca
    16501 ttgcccacct gagcgagcgc gtgcaggccg gcaacctcta tgtgaaccgc aacgtgatcg
    16561 gcgccgtcgt cggcgtgcag ccctttggcg gcatgggtct gtcgggcacg ggccccaagg
    16621 ccggtgggcc gctctacctg caccgcctgg cgcagggcgc gcccaatacc gccctggcag
    16681 cgctgccgcg tgccgaggcc gatcaggcgc agcagcccac gctgcaactg ctcgcgcgcc
    16741 tacagcagac gaccctgccc ggcgtggatg ccgcgctggc gcgcgccgcc tgtgatgcgg
    16801 cgctggccgc ctcgcgccag ggcgcgagct ggctgctgcc cggccccacg ggtgaatcca
    16861 accgctaccg cctgctgccg cgcggccccg tgtgggccct gccgcgcacg ccgctgggcc
    16921 tggtgcacca gctggccgcg gcactggcca gcggcaaccc ttgccatgtg gtgctgcccc
    16981 agggcgacca gggctgcgcc gccgcctggg acgccatcgc cgccgccctg ggcgcggccg
    17041 gccaggactt gctgcgccgc gccgatgccg caagcctgca ggacgccagc acaccggtga
    17101 cggccctgct gttcgaaggt gacggcgacg ccctgctgca ggtgggcgcc ctggtcgccg
    17161 cgcgcgaggg cgccatcgta cgcgtggaaa gccgcagcag cgacgagctc gccgcgggcc
    17221 tgggctacga cctcgcggcg ctggtgcacg agcagtccat cagcaccaac accgcggccg
    17281 cgggcggcaa cgcccagctc atgaccatgg cgtgaggggc cgcgagagac tgatcccaaa
    17341 tccggcagtt accctggagc cggctgccgc aatggcggct tgggtggccc gatgacaggg
    17401 gcgatccggt cgctgatgta gcgcagcaga cagtcccagc ggtccaagtc cttgaactgc
    17461 tccgcagcag ccttgatgtg cagcaaagcc gcgccgatgt tggcgatcag cagcccgagc
    17521 atcaacgtgc ccagcacgtc gcgtttgtcc ggggcgttgc cgctgtggta ggccagcggg
    17581 cagccctgca cccaacgctc gaacacgccg gtggcggcca gaaactgcgc gaagaacacc
    17641 aactggccat gcggcgtggc cgccgcaccc tcgtcccagc gcacgtgcat gcgcccgccc
    17701 agcgtatcca ctacctgcgg cgacgccgcc gcagcctcgg ccgccagggc cacttcgcgc
    17761 ttgcgtttgc tctcacccgt cgggtgagtg tgcccgcagc ggacaaagcc ccgccagtgc
    17821 ttgctcagcg ggcaatcacg agggatgaac cgccggatgt aggctgattc gcgacaagat
    17881 cagcaagggc gactggaagt tgcatgtgga ccgctcgccg tcgctgttcg tcaacgccgc
    17941 cgcctggggc ctggtgctga cgggcaagct caccagtacc agcagcgaga agagcctgtc
    18001 ctcggcgctc acgcgcgtca tcggcaaggg cggcgagccg ctgatccgcc aggcagagcg
    18061 tgcaccgcgc catgaagctc atgggcgaac aattcgtcac cggccagaac gtctccgtcg
    18121 caaaggtgcg ccaggacagg ggacggcgat ggctgtgcgt tctctctcat gccgcggccc
    18181 gatccagcag ggtcccaaac cagtcgagaa atgcggccag tcgccgcgac tggtggtgac
    18241 ggtgcgggta aagggctgtg acggcgacgg gagttgcggg ccagtccggc agcacttcca
    18301 ccagttcgcc gctggcgagc aggtgacgag cgtcgatgcg cggcgcctgg atcagcccga
    18361 tgccggcctg ggcgcaggcc agatagctgt ccatgtcgtc gaccgcgaca cggtgtcgca
    18421 ccgggatgga ctgtgccgcg cccgatgcgc cggtgaaatg ccagaccgcc cggcgtgcct
    18481 cggagggcag caaatagccg atggcccaat gttggaccag ctcgtccggg tgttgcaggg
    18541 ccgcatgggt cgccaaatag cgggggctgg cgcagttgac tcgatccagc tggcccaggg
    18601 gtttgtgcac cagagagtca tcgcgcacat tgcccatccg gatcacgcca tcgacgtcct
    18661 cgccgagcgg atccgggtcg cggtcggaac tgctgaaacg gagttcgagc tcgggatgtt
    18721 gttgcagaaa cgaaggcagc gcgggcgcaa tcaggcgcca gacgatgcgg ttgggcacac
    18781 gaatgtgaag cttgccggcg accacctcct cgtcgttggt gaacagatgg caaatctcgt
    18841 ccaggtcgcc gacgatcatg cgcgccatgg gcagaaatcg ctggccgtcg tgtgtcaggc
    18901 caacctgccg ggtggtgcgg tgcagcaagc gcacgcccag ctcgctttcc agcatccgaa
    18961 tggccaccga aaccgtggcc ggtggaattt tcagggcgcg cgccgcgccg acgaagctgc
    19021 ccgtatcgag gatctgcatg aaaatgcgaa actgatggac ccggtcggac acagggggcg
    19081 gcttgtatgc aggcatcttt cataaacagc gatgaatgag cgctcagttt gtgcagagtc
    19141 gatgattctt tgttttggtt tttgtatgat tcctttgttt tttctgatcg tcggcatgat
    19201 gggcgtcaag gtgcgcatcc ggatggcgcg atcccatggg cggtccttcg gctgcgggcg
    19261 gctgcctttc atgggcggcc agcgtcgcag caaggtgtgc tgaattttca agtttgatcc
    19321 gatgtttttg aatggaattc ggagaaccca tggaagaaaa tcaatctcag tccgcgcatc
    19381 tgctcatcat cgatgacgat ctggatcaac ttcgtctgct ggtcgcggct ttgcgcggca
    19441 cttcacaccg cgtcagcgtg gcgacccagg gtgatcaggg ttacgtgcgg gccatggttt
    19501 tgcagcccaa tttgattttg ctggacgtgt gcatgccggg acgcaacggg atttcggtgg
    19561 cccgtctatt gaaaatcaac ccctccaccc agcatatccc gatcattttt ctttctgcat
    19621 tggtcggcgc gcaagagcgg ttggctggtt tgaaagcagg agctgtcgat tacatcacca
    19681 aaccattttt gatcgaagaa gtcctggagc gaatacgcat tcatctggaa ctggccagaa
    19741 aaaacttgca ccccaatacg catgatttca cgacgaaggg ttcgaatcag gatcccgctg
    19801 ttttcaatcg ccagcctgtg acgctgattt ttagaaaatt ggcgatagaa tacataatgg
    19861 cgcatataaa tgaatcttcc ctgaaggttt ctgatgttgc cgctcatgtg gaaatttcag
    19921 tgtcgaaact gaatgccata ttcgaagcca tcgatggctc ttcggtgttt gaattcatcc
    19981 gcaaggagcg catgcagaga tcggccttga tgctgggcca gagcacactg accatcgcag
    20041 atattgccct ggaagtcggc tattcgaacc ccgcgaattt tactaccgaa ttcagaaaat
    20101 tttggggcag aacgcccatg cagcttcgaa atgaatcaaa aatcaatccg gatgcatggc
    20161 agcaacgcat tgaatccaga tagatgatgg tctgaaatgg atctgaacct ggctaccgct
    20221 gcattcatca ccgctctggt ggagtttgct ctcggggtga gcatgctgat attgtggctg
    20281 agggagggca gacgatatct cgcctactgg agttctggct tcatcgcctt cgggttcggc
    20341 tctctgctca tttcgatgcg caacgaaatt cccgattttc tttccatcat cgttgccaat
    20401 ttcggcacga cactgagttc ggccctgttg tacgtgggta tctgcctgtt ctttggccgg
    20461 cggcactcat ggctgccctg ggtggtgctg gccctggagg cggcatggct ggcctactac
    20521 acctacgttg cgtacgacac ttccgcgcgt gtgtacgtct acagcgtgtc tcaggcgctg
    20581 atcgtgctgg tcaccctcca ggccctgttc atggtgagcc gcgagcgtgc gggccgaagc
    20641 aacccggaag tcgtgatcgt gaccgtgctg ctcctggcgg tgcatcttgc gcgcatcgcc
    20701 ggcacgccgt ttttcccgct gccgcgggac ttcatgaatt caggaaattt ccagactttg
    20761 ctttccttcg ggttgctgat ggttcatgcc agctatgcat tggcgttggg caacatgcac
    20821 gcatccggct tgaatgcgga actgagcgcg gcgcttgctg aagcaaaaat caaggagcgc
    20881 cagaaaatcg aagtcctgag ctatattggt cacgacttgc gtgcgccatt ggcgaccatc
    20941 aatgggtatt ccgagctatt gctagccgat gtgtctgaga gagggcatcg actgttgctt
    21001 tccatccagc gcattgtcaa atatcaagtg gatttgatcg atgagttgct ggaatatgcc
    21061 aaagttgagt tgcgtccgct ggctattcgg tcaaccgcca ccgaccttcc tggcttgttg
    21121 cgaaacatat ccgattatgc gaccaccttg tgtcgccaac aaaacaacca gttcatcttt
    21181 cagccttccg gcagaattcc tcgacaaatc gatatcgatg gaaagcgttt gcagcaggtg
    21241 ctgctgaacc ttatttccaa tgcggccaag ttcacgcacc atggccgggt ggtgctgtca
    21301 atcagggccg agccattgaa agatatctcg gccctgtatt tctccgtgag cgatacgggg
    21361 gttgggattg atctgagccg acagacagat attttcagtg ccttccagga tataaacgct
    21421 gtcaagggcg gcactggctt gggcttgttc attgcgcatc gaattgtttc cgccatgggg
    21481 ggctccttgc gcgttgccag tctgccggga cagggaagta ctttttcatt cgaactttct
    21541 gtccccgttc tggagatgtc cgactttgaa tggcctgctg tggctcagca ggacggggcg
    21601 ctcgtcgcgc cgcccacgga atttatcttt ctggaagacg ccttgccaga tcatcaagcc
    21661 ctggatgagc tggctgatat ggcttctcac ggtcagttca cagatatcga atgctggatc
    21721 gagcgccatg cccaaggcgc tgcgcatgcc gcttttgctg cgcacctgta cgaattgctg
    21781 gaactgttcg atttttcggc aatacgcgcg ctggctttga gctgtcgaaa ccagaaggct
    21841 gccggcaggc cgaatgccca ggaaaactcc tcaaagtaaa tcgcccatct gccagaacca
    21901 cgccaatacc cgcacacgga tgcccgatcg tgccgtcgcg cgtgctcacc cttgtctcgt
    21961 tggccggatg tgcctgatcg gatggaggga atgaattgga catcgacgac actggcgatt
    22021 gtattgaatt tctgaatggt caaaacaaga tctcggttta attcgagatc aaatttcgga
    22081 taacctactt cactttattc ttgcggtaaa agagaggtga aggatggggc ttgcatctat
    22141 attcgttgat cgctttggcg gcgccgcgac cgttcgcggc gcgctggcgg ttgccctgct
    22201 tgggctggga gccatgggca gtgcccaggc tgttcaactc acctccggca gctacccggc
    22261 cagctccaaa ccttatccgg cggtgcacac cgacggcaac acagccgaca accagtgggg
    22321 gaacaacgca acatgaacac aaaatccatc gtggggcgct cgttcgcccg ccatactctc
    22381 ggatgggcgc tactgtgtgc gggctttgcc gcgcacgcgg catgcaccac ctcgaccccc
    22441 atcttcctgt cgcaaggctc tggaggcccc acctcgctgc tgagcagcgc tgcaacgctg
    22501 ccagtcagct tcggctctct gggagcggca gtgagaacct acaacgccat tggctacaac
    22561 gacgtcgacg ataagctgta tggaatctcc aacagcggcc aaatattgct ggaaatcgac
    22621 cagaccaccg gagcctccac cgatgtcggc gccgtttcgg gtcttcccgc cggctcgtac
    22681 aacagcggcg ccttcagcaa caccggtgcc tattacgtca agccttttag caacaacagc
    22741 cagctctacc gaatcgacgt gactgcgtcg ccgcccacgg ctacgctgat tacgctctca
    22801 caggccttca ccacctccga catggcctgg gtcggtagcc tgatgtattc caccgccgac
    22861 aacggacagc tttactccat cgacgtcgcg actggcacca cgacagccat tggctcgccc
    22921 gacaacactg gcggcgtgct gggcgcgcag ttttcgggca ccaatggtct gttcggctcg
    22981 gccaacaatg gctcgggcct gtaccgcatc aacctgacca cgggccagcg ggacaagatt
    23041 tccgattcgc ccgcttcggg ggccaacgac ggtgccagtt gccccacggt ggcgctgttc
    23101 gagcctttgc cacccgagcc gcccgagccg cccgagccgc caccgccccc cacgcccgta
    23161 cccacgctgg acttcgccgg cctgggcctg ctgagcctgc tgggtgctgg tgccggcgcc
    23221 ctggggctgc gccgccgcaa gcgcacgcac tgagcagttg gagcgttgcg acgacgttca
    23281 ggcaccgtct cgcagcgctc cggctcctgc ttctggacct ttcgttctgt cacttctcca
    23341 cttttcttag gatcaagaga tgcaagtttc ccgtactttg cttgcggccg cggccgttgc
    23401 gttgtttggc ttggggccgg tcgctgcgca ggcggccccg ccatacgtca tcgtggctgg
    23461 cacaggtgcc gttggggccg atgtgggcac gttcccgcca ggcacatcgg gcacgattgc
    23521 ggagctgggg gtggactggc aagtgacggc taccgctggc gccaccaccg gctatcacgt
    23581 ctacaacggg gtgcagacct ggtctttcac gcgcaacggt gcgcccgaaa cggtgaatct
    23641 tgaattcgcc atcagcggga ttgatgcgtg gcttaccacg ggcggtgcgg tcgcgacaga
    23701 agccattggt cttccggtgg gcaccgtgtg cgatatcagc aacccagcct atgttttccc
    23761 tgctggcgcg ttggatggaa cgccgggagg gcccgttttt tcctggactc cagacaatgg
    23821 ctcaggcctt gccgtgctgc aatatcttgg caatttgggc ggtgtgccag tcaatcgcta
    23881 tcttcaatat gcccccgttc cctgcaccta caccggcagc caactggtgc tgacgggctc
    23941 tgggttcccg gccaacgaag ccggcggcac gtcgttctgg catggcctgg cttatcttcg
    24001 ggtcatgaac caaccagtgc cgccagctgc ccccacgccc gtacccacgc tggactttgc
    24061 tggcctgggc ctgctgagcc tgctgggtgc tggcgctggc gccctggggc tgcgccgccg
    24121 caagcgcagc aagtaagcgt cggcgcccgt cttcatcggg cgttcaccgc gaacgccgcg
    24181 tggcgaccta cgggttgcca cgcggctttt tgttgggctc atctcggggg tgtggggcgc
    24241 tgtcgcatgt gcctcacccc ggcgccatcg gggtgcgccc caacaccgcc cccagcgcca
    24301 ccccggtgtg cgtgccggcg cgcaccacgt cctcgggcgt gccgacggcg acgatgcggc
    24361 cgccgccgtc gccgccttcg gggcccaggt cgatgatcca gtcggcttcg gcgatcaggt
    24421 ccaggtcgtg ctcgatcacg atcacgctgt ggccgccgcc caccaggcga tgcagcacgc
    24481 ggatcagctt ttcgacgtcc tgcaggtgca ggccgacggt ggattcgtcc agcacgtaca
    24541 gggtgtgggg cgccttgcgg ccgcgtttgg tcacgtcatc gcgcaccttg ctgagttcgg
    24601 tcaccagctt gatgcgctgc gcctcgccgc ccgacagcgt gggcgaggcc tggcccaggg
    24661 tcaggtagcc caggcccacg tcctgcagca gttgcagcgg gtgggcgatg gcaggcatgc
    24721 tggcgaagaa gccgaccgcc tcgtccacct ccatgcgcag cacgtcgccg atgctcttgc
    24781 cgcgccaggt gacggccagg gtctcggggt tgaagcgggc gccgtggcag acctcgcacg
    24841 gcagcttgac gtcgggcaga aagctcatgg ccagggtgcg catgccctgg ccctcgcagg
    24901 cggggcagcg gccgtcgccg gtgttgaaac tgaagcgccc cggcgcgtag ccgcgcg
//
LOCUS       DAOQTZ010000035        24603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2382_length_24603_cov_12.561634, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000035 DAOQTZ010000000
VERSION     DAOQTZ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 24603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24603
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2382_length_24603_cov_12.561634"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PKN51_05985"
     CDS             complement(<1..706)
                     /locus_tag="PKN51_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT84684.1"
                     /translation="MNTQAPITSQPSIGTVPTTRSATSSAASSLWPHFLTGQAEQPVR
                     VILVDDDVHMRHVIAQELLADARIHLLGQAGSLREGKRLVAMQEFDVMLVDLNLGDGT
                     GFQLIEYMKTMHPVAEAVVVSAMEDEDHALHAFELGATGYLIKNSWFGNFPQAVLQVV
                     NGGASITPNLARRLLKRLDGGAVRAPSAQGVRKGSGGEKLSEREREILRLVASGYTSC
                     EIGKRLGISDQTVNTHI"
     gene            1205..1717
                     /locus_tag="PKN51_05990"
     CDS             1205..1717
                     /locus_tag="PKN51_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84685.1"
                     /translation="MKKQLAILAAVVVGTSAWAGGGGGGGGGWGHHGPDICDVCDHDI
                     VIKGPQIQSTSLHNSFSVASARDDSFANNNMSSNTKGVLIKAKSEQTTNLNGTGVLAM
                     ASEHGFAQNNLASNIGSVAVAANQKQTVVTGGLSYIGAYSKDYSTAVQNFSTNNACLD
                     CNPHARRAPR"
     gene            1801..2238
                     /locus_tag="PKN51_05995"
     CDS             1801..2238
                     /locus_tag="PKN51_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84686.1"
                     /translation="MKPNLVLLTLSVAASALGQGVWAQGAPGTDPIVLSAPVRYLAMP
                     TAPFRASEGLDQNMGMPSGLGAKIARIEAKTYAALAGQDGTIYTERDVISRQTGNALE
                     RTCTTDVASNTITPAVGPSGRYGPGTGGDQIAVLRGDVVTICK"
     gene            2349..4013
                     /locus_tag="PKN51_06000"
     CDS             2349..4013
                     /locus_tag="PKN51_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNT84687.1"
                     /translation="MNKPTRISSATSSRPARARMLLLSAVAGAAFVAGCATPLAPKED
                     ALFQSYASASDRPATRPVRSMSSFSDSLACMDEMMREAEVPTMLITSTFIPDFSGRVP
                     VAAKQMVITALSQMSRRSNAFRYVDFEVDIARQDTVQNLTTILLNNNQMELQRPALYV
                     SGGISFVDQNVLSTRHGAGVSGSRVEFGYDRSRGATIVGLEMHLGDFRTRTLIPGLDS
                     ANEVIVGNSGQGLDLAGRIGSYGVQFNVGRDLTQGTGAAMRTLVDLALIELAGKWTHL
                     PYWRCLTLDNTHPEFRRQLREWYDAGDAPARARLVQRYLVARGYLPAFEQPLLPNSAV
                     LREAVGRFQADVGAVVTGDISFDVYERALRDFVAVGEDGQLTRIGWTELANGETKPVP
                     LAISMQIENTVKDRTAFPPGTNIFASVTVSRAAYLYCYLNDTHGNIVKLLPNVLHPDA
                     LVSANQAIRLPDWMVPYPAFVLTSGKQGSEALMCVAANEDPMTRMPQELNASALTPIR
                     GVGTLEDMRKHFETAVGADKMVAQTLQWRILPPPPAPPAAANGGKK"
     gene            4178..5323
                     /locus_tag="PKN51_06005"
     CDS             4178..5323
                     /locus_tag="PKN51_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84688.1"
                     /translation="MTPAAASQPPATVLAQVPAIQLPGSAGMPPADPVANPQALPSPQ
                     LDTHAGPASPALPTARSGAPRLNPAQQQELIRLRRLAEPRSGFGSTQAAANAAWTLGL
                     IELHGGLAPASPAQALVWFERAARYGRQPLAYAGLAWCAIDGCGGRPDPSAAAQALAQ
                     LRPHHRGRALYLQWLLDTRTQPMNVRINGGPEGVNSLLLPFHELLESAAAAGDTQARI
                     ELGLEAVANGNLKDARSHLEAAAPRSRAAAANLKLLELQVAKAAQGAASAPSEADDLY
                     ERARRAHRGIGGPPNYAEALRLYQAAASKGSAAARRMLGFITSRLQPDGTVNLAWMAQ
                     LAWLDSSSTLPRLDTRSLSTMMYRDPTPLFDLLPEPWQRALTSVPSS"
     gene            complement(5344..6261)
                     /locus_tag="PKN51_06010"
     CDS             complement(5344..6261)
                     /locus_tag="PKN51_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT84689.1"
                     /translation="MIQDFKGKTAVLTGAGSGFGLECARIGAARGMNLVLVDVQQDAL
                     ERAAAEMTAAGAQVLARRVDVSSAEQMQALATEVKAHFGAPHFVFNNAGVGAGGLVWE
                     NTVADWNWVLGVDLWGVIHGVRLFVPMMLEAAAQDPAWRGHITNTASMAGLVAPPNMG
                     IYNTAKAAVVNLTETLYHDLNLVTDQIGASLLCPYFVPTGITHSQRNRPDSQAAATLT
                     RSQQIGQAMTEKAVTSGKVSAEQVAQKVFAAISANQFYVFSHESPRALGNISSRMQNM
                     VEMRNPVDPFIEAPEVGARLRAALRAPAS"
     gene            complement(6283..7932)
                     /locus_tag="PKN51_06015"
     CDS             complement(6283..7932)
                     /locus_tag="PKN51_06015"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HNT84690.1"
                     /translation="MSTVTHEVLNQPTPLQDINLFEGHQALRDALRLHAPGLDTASLA
                     SLGKLAGSAEMQRHARLANVHTPELRTHDRFGHRVDEVEFHPSYHALMSAATAAGLHG
                     TPYAADAQAGGHAHLRRAAAFMLFTELEPSILCPISMTYAVTPALRDNPAIYRDWSPQ
                     LTSRAYDPVLRLWRDKPGVTMGMGMTEKQGGSDVRANTTQALPDGHDDWGARYRLTGH
                     KWFMSAPMCDAFLVLAQSAGGLSCFFLPRVLPDGTRNVLRIQRLKDKLGNKANASSEV
                     EFDASLAWLVGEEGRGVPQILAMGVMTRLDCALGTSALMRQALSQALHHTAQRAAFGK
                     RLIEQPLMKNVLADLALESEAATALALRLACAFDRPHDEHERLLARVLTPIAKFWICK
                     RGSHFAQEAMECLGGNGYVEEGGEGVLARIYREMPVNSIWEGSGNIMALDLLRALRKG
                     DVAAALARELAPARGQHAALDRLIETLPTRVDQGADEVGARILARDVALAVQAALLAQ
                     TAPAAVFGAFCASRLKQPHDVFGTLPGGTDFDALIRRAMPA"
     gene            complement(7961..8977)
                     /locus_tag="PKN51_06020"
     CDS             complement(7961..8977)
                     /locus_tag="PKN51_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNT84691.1"
                     /translation="MPTNRQILLDNRPQGEATASNFRLVSSETPALRDGQVLVRHHYL
                     SLDPYMRGRMNESKSYAQPQPLGEVMIGGTVGEVLQSRHPRFQPGDAVVGMGGWQEYS
                     VVDADQPGLLRKVDTTHVPLSYYLGAVGMPGVTAWYGLTQIIAPQAGETVVISAASGA
                     VGSAFAAVAKARGCRVVGIAGGPEKCRYAQEELHFDACIDYRQHPDIKSMAQALKAAC
                     PQGVDGYFENVGGYLLDAVLLRTNAFARIAVCGMIAGYDGQPLPLQNPALILINRLKV
                     QGFIVSEHMDLWPQALQELGSLVAQGRLRPRESIALGIESAPEAFLGLLKGRNFGKQL
                     VKLI"
     gene            complement(9066..9500)
                     /locus_tag="PKN51_06025"
     CDS             complement(9066..9500)
                     /locus_tag="PKN51_06025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNT84692.1"
                     /translation="MSLDFGAHIPFVHHLGFTLERFEGGASTLHFAPRAEHCNSFGVA
                     HGGALMTFLDVVMATAARSANPGSGIVTIEMKTSFMRPAPAAGGVLVGQGRLAHRTRS
                     MAFVEGSVFDAEGQLCAQSTGTFKYVPRAQPTGQPAGPLPTD"
     gene            complement(9626..10420)
                     /locus_tag="PKN51_06030"
     CDS             complement(9626..10420)
                     /locus_tag="PKN51_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT84693.1"
                     /translation="MTRTVQQLFDLKGKTALITGGSRGLGLQMAHALGEAGARLMLSS
                     RKAGDLEQAVAELQAAGIDARWIAADCSKEEDTRRLADETLERMGAIDILVNNAGASW
                     GAAAEEHPVAAWDKVMNLNVRGYFILSQQVANGWMIPNKAGRIINIASIAGLNGNPPE
                     MKTLAYNTSKTAVIGFTRTLAAEWGRFGITVNAICPGFFRTKMANGLIDQLGEGKMAA
                     AAPLGRLGDEEDLKGITLLYASDAGKHITGQWLAVDGGVSVVLGAA"
     gene            complement(10441..10743)
                     /locus_tag="PKN51_06035"
     CDS             complement(10441..10743)
                     /locus_tag="PKN51_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNT84694.1"
                     /translation="MLKHIVMWKFKDQAEGADKAANLAKAVALLEGCADLVPGTLRLE
                     VATAQPGLEATYDLVLYSEFEGRAALDAYQNHPTHVAVKPFLRAVVSERQCMDYEL"
     gene            complement(10743..12575)
                     /locus_tag="PKN51_06040"
     CDS             complement(10743..12575)
                     /locus_tag="PKN51_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNT84695.1"
                     /translation="MSTLRNTLDFLLYDWLAADRLTERPRFADHSRETFDAVLDTCER
                     IAREKYAPFNRLVDTEEPRTETAPDGSLRVVLPRATHEARTAYAESGMLAAAQDHEHG
                     GMQLPYLVEAAANAFFGKASISIGGNLLTVGNANLLMAHGTPLQKQVFAANEFNGRWA
                     GTMCLSEPQAGSSLSDIATRAELEDASDPLGPRYRLRGNKMWISAGEHEMTENIVHLV
                     LAKIPGADGKLVPGVKGISLFIVPKRLVDTEGRLTGVRNDVALAGLNHKLGWRGTTNT
                     LLNFGEGKFPVDGQPGAVGYLVGQPGEGLRCMFHMMNEARIGIGMAATMLGLAGYEAS
                     LDYAKTRIQGRPIGGSGKDAAQPPVRLIEHADIRRMLLAQKSWCEGALALLLYCARLV
                     DEQHTGAPEAADEARLLLEVLTPVAKSWPSENCLEANSLAIQIHGGYGYTRDFPVEQY
                     WRDNRLNMIHEGTHGIQAMDLLGRKVRMEQGRGLALLAARVQATIARAQTHAALQPDA
                     AALGAALDRIQQATRAAWADADANAALANAVPYMQAFGHVVIAWIWLELSLASSTTKT
                     IAAGAGVAGARTYFYRYELPKIDAWLRVVESRDLTCAQLPEEAF"
     gene            12970..15087
                     /locus_tag="PKN51_06045"
     CDS             12970..15087
                     /locus_tag="PKN51_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT84696.1"
                     /translation="MKLPTACPTPTDASRAKLTFRRPRGPLPNLRPTPLALALLGLCA
                     SAQAQTAAPAPADNQLPEIAVTAKGYAADTLDTPASIDVIDAQEIRRKGATSLGQALQ
                     GEPGLAATSDGSASLNPVVRGLKKESLALMVDGMRLNSAQPYGAIGSFVSLGLAERVE
                     VLRGPASVLYGSGALGGAINVLLPQARFDAGVSGRLGLRAASGDRSVGASGVVNWSGG
                     DHALMLGAAGADHGNYRAGGQRVERTGYEQHALIGQYRYRIDGAQQIRLSLQRENLND
                     VWYPGSTQPNANPMVGSTTIHSPRQSRQLMEIGYSHKGRGPVNFDARLYRQSVDRTIN
                     AWANGPLQRDTTTNDVAFDTTGLDVRADWAANEHHLISVGFNGWRMTGSPDRRIFNNG
                     SYVRNNPFEDGWVQAAGLYVQDDMRFGALGVVAGLRYDRVQGNAASVANGSVTTGLKR
                     GDGAVSGNLGLIYELDPLLRPYASLSRGFRPGEMRERFEASPRGDGYFYLGNPQIRPE
                     IAQQIEIGVKGKSEQLEWFAAVYRNRISDYITGRATGQTQNGTPVRQTINLGSVRISG
                     LELGARWQFARGHWLGLGYSRLRGRNNDFNEPLYQMPADELNLSWEGRLGAGWSADAR
                     VRHAWRQDRVATVFARGLEDATPGFTTLDIGATWARGPHQLRLAVTNLTDRKYHEHLA
                     EGLAGREVPAPGRSLVVSYQLQF"
     gene            15093..16094
                     /locus_tag="PKN51_06050"
     CDS             15093..16094
                     /locus_tag="PKN51_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT84697.1"
                     /translation="MTCRRRFLQCTALLGAAPGALLGARAATLPELRLSGPPASVSNP
                     LIHLAAQGRVPGLAERVRYLPWRDPDQLRALALRGEADFVAMPTNVAANLYNRGVPLK
                     LVNVSVWGILFLVSRDAERRTLADFRGEEIAMPFREDMPDLVFQEAAQALGLDPKKDF
                     RLRYVASPLDAMQLLITRRVRHALLAEPAVSMALRKTQSFPVSAIAPELHRAVDIQQE
                     WARAFRRRPRIAQAGIVALGAAAQDAALIARFRDAYAGATAWCQSQRAACGAEVAAVE
                     SRLSAEAVADSLGAAPLDAVPAAQARDELLFLFERLFKRHPQLLGGKLPDAGFLAGA"
     gene            16104..16937
                     /locus_tag="PKN51_06055"
     CDS             16104..16937
                     /locus_tag="PKN51_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT84698.1"
                     /translation="MADRRTAADLTDTRRARWPARVGHYLWSGWTGAASVLLLVAAWD
                     ALARQLGPLVLPTPQETLASLTQILRSPAAWLDLGATARRALYGYGLSVLAGAVLGMA
                     AGASITATMMARPLVTLLIGMPPIAWLVLALLWFGSGDATPVFTVFVACFPIIFVGAL
                     QGTRTLDGRLRELAQAYRLPLTQRFADVYLPHIASYLFAACITALGVSWKVVLMAELL
                     ATSDGAGAQLAATRSHLDMGGTLAWVSAVVGLLLAAEYLLLEPIRREMERWRGDGGGA
                     A"
     gene            16934..17764
                     /locus_tag="PKN51_06060"
     CDS             16934..17764
                     /locus_tag="PKN51_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT84699.1"
                     /translation="MTALALRGVGHRFGPETVLEGIDLQIAPGEVLALLGRSGCGKTT
                     LLRIAAGLLTPTEGQMQTDFSRSAIVFQQPSLLPWKRLLDNIALGLKARGLPRSECHA
                     RAAALAAQVGLRPEDHAKFPAELSGGMQSRAALARAFAVDPALLLLDEPFSALDVGLR
                     AEMHALLARQVGERGCAALLVTHDLMDAVRLAQQLVLLRPDPGRVLARFHLDRPLAAR
                     DDAWVYRQTARLLAHRLVREAFNLPPPVDAPAALPAHEALRASPHPDIPQQVRRHGCG
                     "
     gene            17790..19052
                     /locus_tag="PKN51_06065"
     CDS             17790..19052
                     /locus_tag="PKN51_06065"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNT84700.1"
                     /translation="MLARLWAAPLLCCGFRPFFLGAALWAALAMGLWMLMLTTTLPLV
                     PGPLSGSAWHAHEMVWGFGMAAVTGFLLTAVPEFTATPEFKRRDTALLFGLWLAARLF
                     GLVGLALLPALWVPLTAALNLALVASLAWRSLPRLLRQPLRPHQGFALALLALLGVEA
                     GFWWAALGGRDAMPWVNACVGLMMVLIVLAQSRISMRLLRGMLDHLGVHQADYRAPPP
                     RRRLAIFCIALLTGTELLGVAEPTLGWLALAASAAVLALMSEWHFGRALGHRWVWPLY
                     AVYLCMAAGYALLGLAWLGAPWAPSAGRHALTVGSMGLGVLAVMNIAGRIHAGLPLDD
                     RRWRVVASALLVAAVGARVLASTPWAAGGTLAAWWLAALAWAVAWLLYAGFAWRHLSG
                     PRTDGGTGCEEPLDAAAPAALRPPLAAD"
     gene            complement(19072..19740)
                     /locus_tag="PKN51_06070"
     CDS             complement(19072..19740)
                     /locus_tag="PKN51_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT84701.1"
                     /translation="MLRRLFLTALLGLCSAAALADAHSDEFFRAIRQDNDAAVRSLLA
                     RGFDPNTKDEKGTPGLVMALQEGSLRVADLLIEHPRLVPESRNAADESPLMMAALKGH
                     TELVRRLIARDADVNKPGWAPLHYAATGGHLDIMRQLLEAHAFIDAESPNGTTPLMMA
                     AFYGTPEAVKLLIEAGADIGMRNQKGMTALDFAQRAERQNSIELLQTALRWKLRGGAP
                     RGQW"
     gene            complement(19746..20552)
                     /locus_tag="PKN51_06075"
     CDS             complement(19746..20552)
                     /locus_tag="PKN51_06075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNT84702.1"
                     /translation="MFTDSHCHLTFPELAGDIPAVRAAMAAARVTRALCICTTMEEFP
                     AVQALASGHDNFWATVGVHPDSEQVHEPDVEELVAATTRPRVVAVGETGLDYYQMDER
                     KGGRSIADMGWQRERFRTHIRAARRARVPLVIHTRAAADDTLAILREEGEDGSAGSAG
                     GVFHCFTETRAVARAALDMGFYVSLSGIVTFRNARELHEVATLIPDDRLLIETDAPYL
                     APVPHRGKLNTPAYVPLVAARLAELRGTRTERIGELTSANFTRLFSRVAP"
     gene            complement(20631..21002)
                     /locus_tag="PKN51_06080"
     CDS             complement(20631..21002)
                     /locus_tag="PKN51_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT84703.1"
                     /translation="MSTTPNAAASTPRPSVIQLAIKEKAALYAAYIPMFAEGGIFIPS
                     TRDYRLGDDVYVLITLPDDPQRFPIAGKVAWITPARSVGNRTQGIGIRFPKDEKSAQL
                     KTKIEQILGTALGSDRPTQTI"
     gene            complement(21031..22035)
                     /locus_tag="PKN51_06085"
     CDS             complement(21031..22035)
                     /locus_tag="PKN51_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNT84704.1"
                     /translation="MSGAALAPWIARQHATLRAQRGHAWLLQGPSGLGQFELALALVR
                     AWLCDQPTPDGACGLCPSCHGIDVHTHPDLVVLMPETEMLARGWPLPEKAQAEIDDKK
                     RKPSREIRVDAMREAIEFAQRTSGRGRGKAVLVYPAERMNAVTANALLKTLEEPPGDA
                     RFVLATDAAHLLLPTIRSRCLSHTLAWPPADEALAWLATQGLAGPQAAALLQAAGGRP
                     AEALALAEGKDSARRWSQLAPALARGDVAALADLPLPEAVARLQKLCHDLLALQVGAA
                     PRFFAPADLPRQPLSLLALTRWWQQLAQSARTAEHPLNAGLATEFLVSSARQALNSGR
                     "
     gene            complement(22032..22688)
                     /gene="tmk"
                     /locus_tag="PKN51_06090"
     CDS             complement(22032..22688)
                     /gene="tmk"
                     /locus_tag="PKN51_06090"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNT84705.1"
                     /translation="MAQHGRFISFEGIDGAGKSTHIEALAQALREAGRTVVRTREPGG
                     TPLAEKIRALALHEAMDALTETLLMFAARRDHLTQVIVPALARGEVVLCDRFTDATFA
                     YQGGGRGFDWESLSLLERLAQTVQAPGADLAHEGMVQPDLTIWFDLPPAVAAERLAGT
                     RVPDRFEAQPAAFFEAVRAGYARRASQQPARFARIDADQPREAVWRAVHGAVQARGWL
                     "
     gene            complement(22688..23686)
                     /gene="mltG"
                     /locus_tag="PKN51_06095"
     CDS             complement(22688..23686)
                     /gene="mltG"
                     /locus_tag="PKN51_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNT84706.1"
                     /translation="MRTLLKLLALLLALALAAGAGAWWWTQQPLPLAADALEISVPEG
                     ASLRTAAQRAVDGGVQTPAALLAGYFRLAARGQTIKPGDYALTRGMTPAELLGKLVRG
                     ERIVLTVKLLEGWTFRQLRQALAQAPRLRHDTQDMTPAQIMQALGRPGVAAEGRFFPD
                     TFHYFRNASELDILRQSMQLMEQRLQAAWDARAPDLPLKSADEALVLASIVEKETGRA
                     QDRAEIAGVFINRLRIGMRLQTDPSVIYGLGEGFDGDLKRSHLSADTPYNTYTRAGLP
                     PTPIAMPGKAALLAAVQPAATQALYFVARGDGSSHFSRTLDEHNCAVQRYQRGGSR"
     gene            23734..>24603
                     /locus_tag="PKN51_06100"
     CDS             23734..>24603
                     /locus_tag="PKN51_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HNT84707.1"
                     /translation="MSDTATASFTASALHGIAPLPHLGVIRAHGEDAAHFLQGQLTQD
                     VTGLKPGQARLAALCNPKGRMLASFQVLRRADDELLLVTSADTLAATLKRLSMFVLRA
                     RLKLSDATAELALRGLLGNATTSVAAGAHQTSAGAQNDPAFTISLPAADGAPRALWLA
                     AAGTPPPAGAELSEADWLAAEVRAGVARITRPVVEAFVPQMLNYESVDGVHFQKGCYP
                     GQEVVARSQFRGAIKRRAFVGWADGAVQAGQEVFSASEPEQPAGLIAQAAAVAGGTAV
                     IAALRLDALPTALA"
BASE COUNT         3530 a         8659 c         8580 g         3834 t
ORIGIN      
        1 tgatgtgggt gttgaccgtc tggtcgctga tgcccaggcg tttgccgatc tcgcaactgg
       61 tgtagcccga ggccaccagg cgcaggatct cgcgctcgcg ctccgacagc ttttcgccac
      121 cggagccctt gcgcaccccc tgcgccgatg gcgcgcgcac cgcgccgcca tccagccgct
      181 tgagcaggcg acgcgccagg ttgggcgtga tcgacgcccc gccattgacc acctgcagca
      241 cggcctgcgg aaagttacca aaccaggagt tcttgatcag atagccggtg gcgccgagct
      301 caaaggcatg cagggcgtga tcctcgtcct ccatggccga caccaccacc gcctcggcca
      361 ccgggtgcat ggtcttcatg tattcgatca gctgaaagcc ggtgccatcg cccaggttca
      421 gatccaccag catgacgtcg aactcctgca tggccaccag gcgtttgccc tcgcgcagcg
      481 aaccggcctg gcccagcaga tgaatgcggg catcggccag caactcctgc gcaatgacgt
      541 gccgcatgtg cacgtcgtcg tcgaccagga tcacccgcac cggctgctcg gcttggccgg
      601 tcagaaagtg cggccacagc gacgacgcag ccgaagaggt ggcactgcgc gtggtcggca
      661 cggtgccgat gcttggctgg gacgtgatcg gtgcttgcgt gttcatgacg ttgtcctcct
      721 ggcttccccc cacttgcaat gcagttaaca cggatgccga ctcaccccgt tgatgcaccg
      781 caaaccggct gctgagtttt cattttgatc cgctttgtga ccaaattgaa cgtgaaacag
      841 tagcaccgac acgacaaaaa gtcgagaatt tttcgaaagt aacaattttt cgcacttgta
      901 tccattggag cctacccaac ccacgaggcg cccctgctac gaacctgata gctggccccg
      961 gcttgctgcc atggccagac ccacagtcgc ctgccggggc atgcgtcctc catccgccga
     1021 agccggtagt ccattgaagt gattgccgta ccccagggcc ccgccggaca ctgaccccag
     1081 acgacgccga atgccgacca ccacaactca agggaggcca gacacggcga cgtcaagctc
     1141 cgggacgccg gaatgggccg gcgacccgga tcacctggtt gacaaccttt ggaggacttt
     1201 catcatgaaa aagcaacttg cgatcctggc agccgtggtc gtcggtacca gtgcttgggc
     1261 aggtggtggc ggtggcggtg gcggcggttg gggccaccac ggtcccgaca tctgtgacgt
     1321 ctgcgatcac gacatcgtga tcaagggccc gcagatccag agcacatcgc tgcacaacag
     1381 cttctctgtg gccagcgcca gggacgattc gttcgccaac aacaacatgt cgagcaacac
     1441 caaaggtgtg ctgatcaagg ccaagtctga acagaccacc aacctgaatg gcaccggcgt
     1501 tctggccatg gcaagtgaac atggattcgc acagaacaac ctggccagca acatcggttc
     1561 ggtggcggtg gctgccaacc agaagcagac ggtggtcacg ggcggcctgt cctacatcgg
     1621 cgcctactcg aaagactaca gcacggcagt gcagaatttc tcgaccaaca acgcctgcct
     1681 cgactgcaac ccgcacgcac gcagggcgcc acgctgatgc tctgatggcc tgaagctcgg
     1741 cggaaccgta cgcctgtgcg ccggttccgt cgatgcttcc aaccacaagg ggaacgcgcc
     1801 atgaaaccca accttgtgct cctgacgctg tcggtggcgg catcggcctt gggacagggc
     1861 gtctgggccc agggagcccc gggcaccgac cccatcgtgc tgagcgcgcc agtgcgctat
     1921 ctggcaatgc ccaccgctcc gttcagagcc tccgaggggc ttgaccagaa catgggaatg
     1981 ccatccgggc tgggcgccaa gatcgcgcgc atcgaagcca agacctatgc cgcactcgca
     2041 ggccaggacg gcacgatcta taccgagcgc gacgtgatca gccgtcagac cggcaatgcg
     2101 ctggaacgca cctgcaccac cgatgtggcc agcaacacca tcacgccggc agtgggaccc
     2161 agtggccgct acgggcctgg aaccggcggt gaccagattg ccgtgctgcg aggtgatgtg
     2221 gtgacgatct gcaaatagag agctcgacga cggagccctt cgccgaccga gtccaaaggg
     2281 atctggcacc gaccgcagcg tcgccagatc cctctttttt gatgtttgca aacggcagga
     2341 gaaccgacat gaacaagccc acacgcattt cgagtgccac ctcgtcgcgg cctgcccgtg
     2401 cacgcatgct cctgctgtcg gccgttgccg gtgccgcctt cgtcgctggt tgcgcaacgc
     2461 cgctggctcc caaggaagac gctctgttcc agtcctacgc cagcgccagc gatcgcccgg
     2521 ccacgcggcc ggtgcgctcg atgtcgagct tcagcgactc gttggcctgc atggacgaga
     2581 tgatgcgcga ggccgaagtg ccgacgatgc tcatcaccag caccttcatc cccgatttct
     2641 cgggccgcgt gccggtggcc gccaagcaga tggtgatcac cgccctgtcg cagatgtcgc
     2701 gccgctccaa tgcgttccgc tacgtcgatt tcgaggtcga catcgcgcgc caggatacgg
     2761 tgcagaacct caccaccatt ctgctcaaca acaaccagat ggagctgcag cgtccagcgc
     2821 tgtatgtttc gggcggcatc tccttcgtcg atcagaacgt gctcagcacc cggcacggcg
     2881 ccggcgtgtc gggctcccgc gtcgagtttg gctacgaccg cagtcgcggc gccaccatcg
     2941 tcgggctgga gatgcacctg ggcgactttc gcacgcgcac cttgattccg ggcctggatt
     3001 cggccaacga ggtgatcgtg ggcaactcgg gccaagggct tgacctggcc ggacgcatcg
     3061 gcagctacgg cgtgcagttc aacgtcgggc gtgatctgac ccagggcacc ggcgccgcca
     3121 tgcgcaccct ggtggatctg gccctgatcg aactggccgg caagtggacg cacctgcctt
     3181 actggcgctg cctgacgctc gacaacaccc acccggaatt caggcgccag ctgcgcgagt
     3241 ggtacgacgc cggcgatgca ccggcgcgtg cccggctggt gcagcgttac ctggtcgcgc
     3301 ggggctacct gccggcgttc gagcagccgc tgctgcccaa cagcgccgtc ttgcgcgagg
     3361 ccgtcggccg ctttcaggcg gacgtgggcg ccgtggtgac gggtgacatc agcttcgatg
     3421 tctacgagcg cgccctgcgc gacttcgttg ccgtcggcga ggatgggcaa ctcacacgca
     3481 tcggctggac cgagctcgcc aacggcgaga ccaagccggt gccgctggcg atcagcatgc
     3541 agatcgaaaa caccgtcaag gaccgcacgg cgttcccgcc cggcaccaac atctttgcct
     3601 cggtcacggt ctcccgggcg gcctacctgt actgctacct gaacgacacc catggcaaca
     3661 tcgtcaagtt gctgcccaac gtcctgcacc cggacgcgct ggtgtctgcc aatcaggcga
     3721 ttcgcctgcc cgactggatg gtgccctacc cggcgttcgt gctgacctcg ggcaagcagg
     3781 gcagcgaagc cctgatgtgc gtcgccgcca acgaggaccc gatgacgcgc atgccacagg
     3841 agctcaacgc cagcgccttg acgcctatcc gcggggtcgg tacgctagaa gacatgcgca
     3901 aacattttga aaccgccgtc ggtgccgaca agatggtggc gcagacgctc cagtggcgga
     3961 tcctcccacc gccgccggcc ccgcccgcgg ccgccaacgg tggcaagaaa tgacctgctg
     4021 gggcgtcatg ggtgaactgg tccaggcatg gccgaccgtg cttgccaggc gggttcgcag
     4081 ggcctggcgg gccggtgggt gtctgctcgg tctgctgatc gcgggcgcga ccgcgcaggc
     4141 agcgaccgtg cgggcgacga tcctgccggc catcaccatg acgccagccg cagcttccca
     4201 accgcccgcc accgtgctgg cgcaggtgcc ggccatccaa ctgcccggca gcgcgggcat
     4261 gccccccgcc gatccggtcg ccaaccccca ggcgttgccc tcaccgcagc ttgacacgca
     4321 tgccggcccc gcgtcgccgg cgctgcctac ggcccgaagc ggcgcgccgc gcctcaaccc
     4381 ggcacagcag caggagctga tccgtctgcg ccgcctggcc gagccgcgca gtggtttcgg
     4441 ctccacccag gccgccgcca acgccgcctg gaccctgggt ctgatcgagc tacacggggg
     4501 cctggcgccg gcttcgcccg cgcaggcgct ggtctggttc gagcgcgcgg cgcgctacgg
     4561 gcgccagccg ctggcctacg ccggtctggc ctggtgtgcc atcgacggct gcggtggtcg
     4621 cccggacccc tcggccgcgg cgcaggccct ggcccagctt cgaccgcacc accgcggccg
     4681 ggcgctgtat ctgcaatggc tgctggacac ccgcacccag ccgatgaacg tgcgcatcaa
     4741 cggcggcccg gaaggcgtca acagcctgct gctgccattt cacgaactgc tggaaagcgc
     4801 tgccgccgcc ggcgacacgc aggcacgcat cgagctgggg ctggaagccg tggccaacgg
     4861 caacctgaag gacgcgcgca gccatctgga agcggcggca ccgcgctcgc gcgccgcggc
     4921 cgccaacctg aagctgctgg agctgcaggt ggccaaagcc gcccaggggg ccgcgtccgc
     4981 cccttcggaa gcggacgatc tgtacgaacg cgcccggcgg gcgcatcgcg gcatcggcgg
     5041 cccacccaac tacgccgagg ccctgcgcct gtatcaggcc gccgccagca agggcagtgc
     5101 cgccgcgcgg cgcatgctgg gtttcatcac ctcgcgcctg caaccggacg gcaccgtcaa
     5161 cctggcctgg atggcccagc tggcctggct cgacagctcc agcaccctgc ccaggctgga
     5221 tacgcgctcg ctgtcgacca tgatgtaccg cgacccgacg ccgctgttcg acctgctgcc
     5281 cgagccgtgg cagcgcgccc tcacgagcgt gccatcgtcc tgagccggcg caacccggcg
     5341 gcctcagctg gccggagcac gcagcgccgc gcgcaaccgg gcacccactt ccggcgcctc
     5401 gatgaatggg tcgaccgggt tgcgcatctc caccatgttc tgcatgcggc tgctgatgtt
     5461 gcccagggcg cgcgggcttt cgtggctgaa gacgtagaac tggttggcgc tgatggcggc
     5521 aaacaccttc tgcgccacct gctcggccga caccttgccg ctggtgacgg ctttttcggt
     5581 catggcctgc ccgatctgct ggctgcgcgt cagggtggcc gcggcctggc tgtcgggtcg
     5641 gttgcgctgg ctgtgggtga tgccggtggg cacgaaatac gggcacagca ggctggcgcc
     5701 gatctggtcg gtcaccaggt tcaggtcgtg gtagagcgtt tcggtcaggt tgaccaccgc
     5761 cgccttggcg gtgttgtaga tgcccatgtt gggcggcgcc accagaccgg ccatgctggc
     5821 ggtgttggtg atgtggccgc gccaggccgg gtcctgcgcc gcggcctcca gcatcatggg
     5881 cacgaacagg cgcacgccgt ggatcacgcc ccacaggtcc acccccagca cccagttcca
     5941 gtcggcgacg gtgttttccc acaccagacc gcccgcgccc acgccggcgt tgttgaagac
     6001 gaagtgcggc gcgccgaagt gcgccttgac ctcggtggcc agggcctgca tctgctcggc
     6061 gctggataca tcgacccggc gcgccagcac ttgggcgcct gcggccgtca tctcggctgc
     6121 ggcgcgctcc agggcgtcct gctgcacgtc caccagcacc aggttcatgc cccgggcggc
     6181 gccgatgcgc gcacattcca gcccgaaacc ggagccggcg ccggtcagca ccgccgtctt
     6241 gcccttgaag tcttgaatca tgtgggttcc tcttgaaccg gctcaggccg gcatggcgcg
     6301 ccggatcagg gcgtcgaaat cggtgccacc gggcagggtg ccgaacacgt cgtgcggctg
     6361 tttcaggcgc gaggcgcaga aggcgccgaa caccgccgcc ggcgccgttt gcgccagcag
     6421 cgcggcctgc accgccagcg ccacgtcgcg cgccaggatg cgggcgccga cctcatcggc
     6481 gccttggtcc acgcgggtgg gcagggtttc gatcagccgg tccagcgccg catgctggcc
     6541 gcgcgcgggc gccaactcgc gcgccagggc ggcggcgacg tcgcccttgc gcagcgcgcg
     6601 cagcagatcg agtgccatga tgttgcccga gccttcccaa atgctgttga ccggcatctc
     6661 gcggtagatg cgcgccagca cgccttcacc gccctcctcc acgtagccgt tgccgcccag
     6721 gcattccatg gcttcctggg caaagtggct gccgcgtttg cagatccaga acttggcgat
     6781 cggcgtcagc acgcgcgcca gcaggcgctc gtgttcgtcg tgcgggcggt cgaaggcgca
     6841 ggccagccgc agcgccaggg cggtggcggc ttcgctttcc agcgccaggt cggccagcac
     6901 gttcttcatc aggggctgct cgatcagccg cttgccgaag gccgcgcgct gcgccgtgtg
     6961 gtgcagtgcc tggctgagcg cctggcgcat cagggcgctg gtgcccaggg cgcagtccag
     7021 ccgcgtcatc acgcccatgg ccaggatctg cggcacgccg cgcccttctt cgcccaccag
     7081 ccaggccagc gaagcgtcga attcaacctc ggagctggcg ttggccttgt tgcccagctt
     7141 gtccttcagg cgctgaatgc gcagcacgtt gcgcgtgcca tcgggcagca cgcgcggcag
     7201 gaagaagcac gacaggccgc cggccgactg cgccagcacc aggaaggcgt cgcacatcgg
     7261 tgccgacatg aaccacttgt gcccggtcag gcggtagcgc gcgccccagt cgtcgtggcc
     7321 gtcgggcagc gcctgggtgg tgttggcgcg cacgtcggag ccgccctgct tttcggtcat
     7381 gcccatgccc atggtgacgc cgggcttgtc gcgccacagg cgcagcaccg ggtcgtaggc
     7441 gcggctggtg agctgcggcg accagtcgcg gtagatggcc gggttgtcgc gcagcgcggg
     7501 cgtgacggcg taggtcatcg agatcgggca caggatggag ggttcgagct cggtgaacag
     7561 catgaacgcc gccgcgcgcc gcaggtgggc gtggccgccg gcctgcgcgt cggccgcgta
     7621 cggcgtgcca tgcaggccgg cggcggtggc ggccgacatc agggcgtggt agctggggtg
     7681 gaactcgacc tcatccaccc ggtggccaaa gcggtcgtgc gtgcgcagct cgggcgtgtg
     7741 cacgttggcc aggcgcgcgt ggcgctgcat ctcggcgctg ccggccagct tgcccaggga
     7801 cgccaggctg gcggtgtcga gcccgggggc atgcaggcgc agcgcgtcgc gcagggcctg
     7861 atggccctcg aacaggttga tgtcctgcag cggcgtgggc tggttcagta cctcgtgcgt
     7921 gacggtgctc atgggcgggt ctccgaaggc aaacggtcga tcagatcagt ttcaccagtt
     7981 gcttgccgaa gttgcggccc ttgagcaagc ccagaaaggc ctctggcgcg ctttcgatgc
     8041 ccagggcgat cgattcgcgc gggcgcaacc tgccctgcgc caccagcgaa cccagctcct
     8101 gcagcgcctg cggccagagg tccatgtgct cgctgacgat aaagccctgc accttgagcc
     8161 ggttgatgag gatcaaagcc gggttctgca gcggcagcgg ctggccgtcg tagccggcga
     8221 tcatgccgca cacggcgatg cgcgcgaagg cgttggtgcg cagcagcacg gcgtcgagca
     8281 agtagccacc gacgttctcg aaatagccgt cgacgccctg cgggcaggcg gccttcagcg
     8341 cctgcgccat ggacttgata tcggggtgct ggcggtagtc gatgcaggcg tcgaagtgca
     8401 gctcctcctg cgcgtagcgg cacttttccg ggccgccggc gatgcccacc acccggcagc
     8461 cgcgcgcctt ggccacggcc gcgaaagcgc tgccgacggc gccgctggcg gcgctgatca
     8521 ccacggtttc gcccgcctgc ggggcgatga tctgcgtcag cccgtaccag gcggtgacgc
     8581 ccggcatgcc gaccgccccc aggtagtacg acagcggcac atgggtggtg tcgaccttgc
     8641 gcagcaggcc gggctggtcg gcatcgacca cgctgtattc ctgccacccg cccatgccca
     8701 ccacggcgtc gcccggctga aagcgtgggt ggcgcgactg cagcacctcg cccaccgtgc
     8761 cgccgatcat cacctcgccc agtggctggg gctgggcgta gctcttgctt tcgttcatgc
     8821 gaccgcgcat gtacgggtcg aggctcaggt aatggtggcg caccagcacc tggccgtcgc
     8881 gcagcgctgg ggtttcgctg gacaccaggc gaaagttgct ggccgtggct tcgccctgcg
     8941 ggcggttgtc gagcaggatc tggcggttgg tgggcatgcg ggtcctcgat cggtggacta
     9001 tggaaagaat agctgctggc gcttgtctgg tgggcgctgg cggccgaaaa aacctgaaag
     9061 cagcgtcagt cggtgggcaa tggcccggcg ggctggcccg tcggctgggc gcgtggcacg
     9121 tacttgaagg tgccggtgga ctgggcgcac agctgacctt cggcatcgaa caccgagcct
     9181 tcgacgaacg ccatgctgcg cgtgcggtgc gccagccggc cctgccccac cagcacgccg
     9241 ccggccgccg gagccggtcg catgaagctg gttttcatct cgatggtgac gatgccgctg
     9301 ccggggttgg cgctgcgcgc ggcggtggcc atgacgacgt ccagaaaggt catcagcgcg
     9361 ccgccgtggg cgacgccgaa ggagttgcag tgctcggccc ggggcgcgaa gtgcagcgtt
     9421 gatgcgccgc cttcgaagcg ctccagcgtg aagcccaggt gatggacgaa gggaatgtgg
     9481 gcgccgaagt ccaggctcat ggtcaaagtt cagttgacgc ttgtgaaagg cagcccggcc
     9541 gccgccgagc cgctcccaag gcggccggag ccccctcggg gggcagcgca ccacgcgcag
     9601 cgggggagga gcgtgggtgc cagattcatg ccgcccccag caccacgctg acgccgccgt
     9661 ccaccgccag ccactggccg gtgatgtgct tgccggcgtc gctggcgtac agcagggtga
     9721 tgcccttcag gtcttcctca tcgcccaggc ggcccagcgg cgcggcggcg gccatcttgc
     9781 cctcgcccag ctggtcgatc aggccattgg ccattttggt gcggaaaaaa cccgggcaga
     9841 tggcgttgac ggtgatgcca aagcgccccc actcggccgc cagcgtgcgc gtgaagccga
     9901 tgacggcggt cttgctggtg ttgtaggcga gggtcttcat ctcgggcggg ttgccgttca
     9961 ggccggcgat ggaggcgatg ttgatgatgc gcccggcctt gttgggaatc atccagccgt
    10021 tggcgacctg ctggctgagg atgaagtagc cgcgcacgtt caggttcatc accttgtccc
    10081 aggcggccac cgggtgctcc tcggccgccg cgccccagct ggcgccggcg ttgttgacca
    10141 ggatgtcgat ggcgcccatg cgttcgagcg tttcgtcggc caggcggcgc gtgtcttctt
    10201 ccttgctgca gtcggcggcg atccagcgcg cgtcgatgcc ggcggcctgc agctcggcca
    10261 cggcctgctc caggtcgccc gccttgcgcg agctgagcat caggcgcgcg ccggcctcgc
    10321 ccagggcgtg cgccatctgc aagcccaggc cgcgcgagcc gccggtgatg agggcggtct
    10381 tgcccttaag gtcgaacagt tgctgtacgg tgcgggtcat gtcggatcct ggtcgcgggt
    10441 tcagagctcg tagtccatgc actggcgctc gctcaccacg gcgcgcagaa agggtttgac
    10501 ggccacgtgg gtggggtggt tctgataggc atccagcgcc gcgcggcctt caaactcgct
    10561 gtagagcacc aggtcgtagg tggcttccag ccccggctgg gcggtcgcca cctcgagccg
    10621 cagcgtgccg ggcaccagat cggcacaacc ctccagcaga gcgacggcct tggccaggtt
    10681 ggcggccttg tcggcgcctt cggcctggtc cttgaatttc cacatgacga tgtgcttgag
    10741 catcagaagg cctcctcggg cagttgggcg caggtcaggt cgcggctttc caccacgcgc
    10801 agccaggcgt cgattttggg caattcatac cgataaaaat aggttctggc gcccgccaca
    10861 cctgcgccag cagctattgt ttttgtagta cttgaagcga gcgacagctc cagccagatc
    10921 caggcgatga ccacatggcc gaaggcctgc atgtagggca cggcgttggc cagcgcggcg
    10981 ttggcgtcgg cgtctgccca ggcggcgcgg gtggcctgct gaatgcgatc gagcgcggcg
    11041 cccagcgcgg cggcgtcggg ctgcagcgcc gcgtgcgtct gcgcccgcgc aatggtggcc
    11101 tgcacgcgcg cggccagcag cgccagcccc cggccctgct ccatgcgcac cttgcgcccc
    11161 agcaggtcca tggcctggat gccgtgcgtg ccctcgtgga tcatgttcag gcggttgtcg
    11221 cgccagtact gctcgaccgg gaagtcgcgc gtgtagccgt agccgccgtg gatctggatg
    11281 gccaggctgt tggcctccag gcagttttcg ctcggccagc tcttggccac gggggtcagc
    11341 acctccagca gcaggcgcgc ctcgtcggcc gcttcgggcg cgccggtgtg ctgctcgtcg
    11401 accagacggg cgcagtacag cagcagcgcc agcgcgcctt cgcaccagct tttctgcgcc
    11461 agcagcatgc ggcggatgtc ggcgtgctcg atcaggcgca ccggcggctg ggcggcatct
    11521 ttgccgctac caccgatggg ccggccctgg atgcgcgtct tggcgtagtc gagcgaagcc
    11581 tcgtagccgg ccaaccccag catggtggcg gccatgccga tgccgatgcg cgcctcgttc
    11641 atcatgtgga acatacagcg caggccctcg cccggctggc ccaccaggta gcccacggcg
    11701 ccgggctggc cgtcgaccgg gaacttgccc tcgccaaagt tgagcagcgt gttggtggtg
    11761 ccgcgccagc ccagtttgtg gttcaggccg gccaaggcga catcgttgcg cacgccggtc
    11821 aggcggcctt cggtgtccac cagccgcttg ggcacgatga acagcgaaat gcccttgacg
    11881 cccggcacca gcttgccgtc ggcgccgggg atcttggcca gcaccaggtg cacgatgttt
    11941 tcagtcatct cgtgctcgcc ggccgagatc cacatcttgt tgccacgcag gcggtagcgc
    12001 gggcccagcg ggtcgctggc gtcttccagc tcggcgcgcg tggcgatgtc gctcaggctg
    12061 gagccggcct gcggctcgct caggcacatg gtgccggccc agcggccatt gaattcgttg
    12121 gcggcaaaca cctgtttttg cagcggcgtg ccgtgcgcca tcaggaggtt ggcgttgccc
    12181 acggtgagca ggttgccgcc gatgctgatc gaggccttgc cgaagaaggc gttggccgcc
    12241 gcctcgacca ggtagggcag ctgcatgccg ccgtgctcat gatcctgcgc cgccgccagc
    12301 atgccggatt cggcgtaggc ggtgcgcgcc tcgtgggtgg cgcgtggcag caccacgcgc
    12361 aggctgccat cgggtgcggt ttcggtgcgt ggttcttcgg tgtcgaccag gcgattgaaa
    12421 ggggcgtact tttcgcgcgc gatgcgctcg caggtgtcga gcacggcgtc gaaagtctcg
    12481 cgcgagtggt cggcaaatcg tgggcgctcg gtcagccggt cggccgccag ccagtcgtag
    12541 agcagaaagt cgagggtgtt gcgcagggtg gacatcgcgc cgattatggg gcgccccctg
    12601 cgctgcgctt gtccgctgcg tgacgtgggc catgccgccc ggcacggagg cgcccggccc
    12661 cgccgcggcc cctggcgcca cggccaccct ttcacgcccc actctcgggt gaacttgttt
    12721 cagatcaagg ttgaacggtg tcgaatggtg aatgattcaa tgataatcat tcgcattcga
    12781 accgatgcac ccgacagccc ccgatcgccc cacccctgcc acggcatggg cgcgcgggcg
    12841 tgcgcccgct ggcggcatgc gagcgctcag cgcgcgccgc cggggcatcg gtgaccttca
    12901 tcagccagcc agcagcccgg tctgtccgcc agcgattgcc cttcctccat cgccgttacg
    12961 gaccctccca tgaagctgcc caccgcctgc ccgacgccca ccgacgccag ccgagccaag
    13021 ctcaccttcc gccggccccg cggaccactg ccgaatcttc gcccgacccc gcttgccctg
    13081 gcgctgcttg gcctgtgcgc cagcgcgcag gcccagaccg cggcgccagc gccagccgac
    13141 aaccagctgc ccgaaatcgc cgtcacggcc aagggctacg cggcagatac gctggacacc
    13201 ccggcgtcga tcgacgtcat cgacgcccag gagatccggc gcaagggcgc caccagcctg
    13261 ggccaggccc tgcagggcga gcccggcctg gccgccacca gcgacggcag cgccagcctg
    13321 aacccagtgg tgcgcgggct caagaaggaa tcgctggcgc tgatggtcga cggcatgcgc
    13381 ctgaactcgg cccagcccta tggcgccatc ggcagcttcg tctcgctcgg cctggccgag
    13441 cgggtggaag tgctgcgcgg gccggcctcg gtgctgtatg gctcgggcgc gctgggcggc
    13501 gccatcaacg tgttgctgcc ccaggcgcgt ttcgacgccg gcgtctccgg ccgcctgggc
    13561 ctgcgcgcgg ccagtggcga ccgcagcgtc ggtgccagcg gcgtggtgaa ctggagcggc
    13621 ggtgaccacg ccctgatgct gggagctgcc ggtgccgacc acggcaacta ccgcgccggc
    13681 gggcagcgcg tcgagcgcac cggctacgag cagcacgcgc tgatcggcca gtaccgctac
    13741 cgcatcgacg gcgcccaaca gatccgcctg tcgctgcagc gcgagaacct gaacgacgtc
    13801 tggtaccccg gttcgaccca gcccaacgcc aaccccatgg tgggctccac caccattcac
    13861 tcgccgcgcc agtcgcgcca gctgatggag atcggctaca gccacaaggg gcgcgggccg
    13921 gtcaacttcg atgcacggct gtaccgccag agcgtcgatc gcaccatcaa cgcctgggcc
    13981 aacggcccgc tgcagcgtga caccaccacc aacgacgtcg ccttcgacac caccggcctg
    14041 gacgtccgcg ccgactgggc ggcgaacgaa caccatctga tcagcgtcgg cttcaacggc
    14101 tggcgcatga cgggctcgcc cgaccgccgc atcttcaaca acggcagcta cgtgcgcaac
    14161 aacccgttcg aggacggctg ggtgcaggcc gccggcctgt acgtgcagga cgacatgcgc
    14221 ttcggcgcgc tgggcgtggt ggccggtctg cgttacgacc gggtgcaggg caacgccgcc
    14281 tcggtggcca acggcagcgt caccaccggt ctgaagcgcg gcgacggcgc cgtgtcgggc
    14341 aacctgggcc tgatctacga actcgacccg ctgctgcgcc cctacgccag cctgtcgcgc
    14401 ggcttccggc cgggcgagat gcgcgagcgc ttcgaggctt cgccgcgcgg cgacggctac
    14461 ttctacctgg gcaacccgca gatccggccc gagatcgccc agcagatcga gatcggcgtc
    14521 aagggcaagt ccgagcagct ggagtggttc gccgccgtct accgcaaccg catcagcgac
    14581 tacatcaccg gtcgcgccac cggccagacc cagaacggca ccccggtgcg ccagaccatc
    14641 aacctgggca gcgtgcgcat ctccggcctg gagctgggcg cccgctggca gtttgcgcgt
    14701 ggccactggc tgggcctggg ctattcccgt ctgcgcggcc gcaacaacga cttcaacgaa
    14761 ccgctctacc aaatgcccgc cgacgagctt aacctgagct gggaaggccg cttgggcgcc
    14821 ggctggagcg ccgacgcgcg agtgcgccac gcctggcgcc aggaccgcgt cgccaccgtg
    14881 ttcgcacgtg ggctggaaga cgccacgccc ggcttcacca cgctcgatat cggcgccacc
    14941 tgggcgcgcg gcccgcacca gctgcgcctg gccgtcacca acctgaccga ccgcaaatac
    15001 catgagcacc tggccgaggg cctggcagga cgcgaagtgc cggcgccggg ccgctcgctg
    15061 gtcgtcagct accagctcca gttctaatcg ccatgacctg tagacgtcgt ttcctgcaat
    15121 gcaccgcgct gctgggtgcc gcgcccggcg cgctgctggg ggcgcgtgcc gccacgctgc
    15181 ccgagctgcg cctgtccggc ccgccggcca gcgtcagcaa cccgctgatc cacctggccg
    15241 cccagggccg cgtgcccggc ctggccgaac gcgtgcgcta cctgccctgg cgcgaccccg
    15301 accagctgcg cgcgctggcg ctgcgtggcg aggccgactt cgtcgccatg ccgaccaacg
    15361 tcgccgccaa cctgtacaac cggggcgtgc cgctgaagct ggtcaacgtg tcggtgtggg
    15421 gcatcctgtt cctggtctcg cgcgatgccg aacgccggac gctggccgac ttccgcggcg
    15481 aggaaatcgc catgccgttt cgcgaggaca tgcccgatct ggtgtttcag gaagcggcgc
    15541 aggccctggg gctggacccg aaaaaggact tccgcctgcg ctacgtcgcc tcgccgctgg
    15601 acgccatgca gctgctcatc acgcggcgcg tgcgccacgc cctgctggcc gagccggcgg
    15661 tgtcgatggc gctgcgcaag acgcagtcct tcccggtcag cgccatcgcc cccgagttgc
    15721 atcgcgccgt cgacatccag caggaatggg cacgcgcctt caggcgccgg ccgcgcatcg
    15781 cccaggccgg catcgtggcg ctgggggcgg cagcgcagga cgcggcgctg attgcccgct
    15841 tccgtgacgc ctacgccggc gccaccgcct ggtgccagag ccagcgcgcc gcctgcggcg
    15901 ctgaggtggc ggcggtcgag tcgcgcctgt cggccgaagc cgtggccgac agcctgggcg
    15961 ccgcgccgct ggacgccgtg ccggcggcgc aggcgcgcga cgagctgctg ttcctgttcg
    16021 agcgcctgtt caagcgccac ccacagctgc tgggtggcaa actgccggac gccggcttcc
    16081 ttgccggcgc ctgaagcgca tcgatggccg accgccgcac cgccgccgac ctgaccgaca
    16141 cccgccgcgc ccgctggccg gcgcgcgtcg gccactatct gtggagcggc tggaccggcg
    16201 ccgccagcgt gctgctgctg gtggccgcct gggacgcgct ggcgcgccag ctggggccgc
    16261 tggtgctgcc gacgccgcag gagaccctgg ccagcctgac ccagatcctg cgatcgcccg
    16321 ccgcctggct ggacctggga gccacggcgc ggcgcgccct ctacggctac ggcctgtcgg
    16381 tgctggccgg cgccgtgctg ggcatggcgg ccggcgcctc gatcaccgcc accatgatgg
    16441 cgcggccgct ggtcacgttg ctgatcggca tgccgccgat cgcctggctg gtgctggctc
    16501 tgctgtggtt cggcagcggc gacgcgacgc cggtgttcac cgtcttcgtg gcctgcttcc
    16561 cgatcatctt cgtcggcgcc ctgcagggca cgcgcacgct cgacggccgt ctgcgcgagc
    16621 tggcgcaagc ctatcgcctg ccgctgacgc agcgctttgc cgacgtctac ctgccgcaca
    16681 tcgcctccta cctgttcgcc gcctgcatca cggcgctggg ggtgtcgtgg aaggtggtgc
    16741 tgatggccga gctgctggcc acctccgacg gcgccggcgc gcaactggcg gccacccgca
    16801 gccacctcga catgggcggc acgctggcct gggtgagcgc cgtggtcggc ctgctgctgg
    16861 cggccgagta cctgctgctg gagccgatcc gacgcgagat ggagcgctgg cgcggtgacg
    16921 gcggaggcgc ggcatgacgg cgctggcgct gcgcggcgtg ggccaccgct tcggccccga
    16981 gacggtgctg gaggggatcg acctgcaaat cgccccgggc gaggtgctgg ccctgctggg
    17041 ccgctcgggc tgcggcaaga ccacactgct gcgcatcgcc gccggcctgc tgacgcccac
    17101 cgagggtcag atgcagaccg acttctcgcg cagcgccatc gtgttccagc aacccagcct
    17161 gctgccctgg aagcgtctgc tcgacaacat cgcgctgggc ctgaaggcgc gcggcttgcc
    17221 gcgcagcgaa tgccacgcgc gcgccgccgc gctggcagcg caggtcggcc tgcgcccgga
    17281 agaccacgcc aagttcccgg ccgagctgtc cggcggcatg cagagccgcg ccgcgctggc
    17341 acgcgcgttc gcggtcgatc cggcgctgct gctgctggac gagccgtttt cggcgctcga
    17401 cgtcggcctg cgcgccgaga tgcacgccct gctggcgcgg caggtcggcg agcgcggctg
    17461 cgccgccctg ctggtcacgc acgatctgat ggacgccgtg cgcctggcac agcaactggt
    17521 gctgctgcgc cccgaccccg gccgcgtgct ggcgcgcttt cacttggata ggccgctggc
    17581 cgcgcgcgac gacgcctggg tctaccggca gaccgcgcgc ctgctggcgc accgactggt
    17641 gcgcgaagcc ttcaacctgc cgccgccggt ggatgcgccg gccgcgctgc cggcccacga
    17701 ggcgctgcgg gccagccccc accccgacat cccccagcag gtgcgtcgcc atggctgtgg
    17761 ctgaaccccg cggcgcggcc ccgcgcccct tgcttgcgcg tctgtgggct gcgccactgc
    17821 tgtgctgcgg ctttcggccc ttctttctcg gagcggcgct gtgggcggcg ctggccatgg
    17881 gcttgtggat gctgatgctg accacgaccc tgccgctggt gccggggccg ctcagcggca
    17941 gcgcctggca cgcccatgaa atggtgtggg gtttcggcat ggccgcggtg acggggtttc
    18001 tgctgacggc ggtgccggaa ttcaccgcca cgccggaatt caagcggcgc gacaccgcgc
    18061 tgctgttcgg tctctggctg gcggcgcgcc tgttcgggct ggtcggcctg gccctgctgc
    18121 ccgccctgtg ggtgcccctg acggcagcgc tgaacctggc gctggtcgcc agcctggcct
    18181 ggcgcagcct gccgcgcctg ctgcgccagc ccctgcgccc gcaccagggc tttgcgctgg
    18241 cgctgctggc cctgctgggc gtggaggcgg gcttctggtg ggccgcgctc ggcgggcgcg
    18301 acgccatgcc ctgggtcaac gcctgcgtcg gcctgatgat ggtgctgatc gtgctggcgc
    18361 aaagccgcat atccatgcgc ctgctgcgcg gcatgctgga ccacctgggc gtgcaccagg
    18421 ccgactaccg cgcacctcca ccccggcgcc ggctggcgat cttctgcatc gcgctgctga
    18481 cggggacgga gctgctgggc gtggccgagc ccacgctggg ctggctggcg ctggcggcgt
    18541 cggcggcggt gctggcgctg atgagtgaat ggcatttcgg ccgcgccctc gggcaccgct
    18601 gggtgtggcc gctctacgcg gtctacctgt gcatggcggc cggctatgcc ctgctgggcc
    18661 tggcctggct gggggcgccg tgggcgcctt cggccgggcg ccatgccctg accgtgggca
    18721 gcatgggcct gggcgtgctg gcggtgatga acatcgccgg ccgcatccac gccggcctgc
    18781 cgctcgacga tcgccgctgg cgcgtggtgg ccagcgccct gctggtggcg gcggtgggtg
    18841 cgcgcgtgct ggcgagcacg ccctgggcgg cgggcggcac gctggcggcg tggtggctgg
    18901 cggcgctggc ctgggccgtg gcctggctgc tctacgccgg ctttgcctgg cgccacctga
    18961 gcgggccgcg caccgacggt ggcaccggct gcgaggaacc gctggacgcg gccgcgccgg
    19021 cggcactgcg gccgcccttg gccgccgatt gagcgcgccc ggcgccggcg ttcaccactg
    19081 gccgcgtggt gcgccaccac gcagcttcca gcgcagcgcg gtctgcagca gctcgatcga
    19141 gttctggcgc tcggcgcgct gggcgaagtc gagcgccgtc atgcccttct ggttgcgcat
    19201 gccgatgtcg gcgccggcct cgatcagcag cttgaccgcc tcgggcgtac cgtagaaggc
    19261 cgccatcatc agcggcgtgg tgccgttggg cgattcggcg tcgatgaagg catgcgcttc
    19321 cagcaactgg cgcatgatgt ccaagtgccc gccggtggcg gcgtagtgca gcggcgccca
    19381 gccgggcttg ttgacgtcgg catcgcgcgc gatgagccgg cgcaccagct cggtgtggcc
    19441 tttcagcgcc gccatcatga gcgggctttc gtcggcggcg ttgcggctct cgggcaccag
    19501 gcgcgggtgc tcgatcagca ggtcggccac gcgcagcgag ccttcctgca gcgccatcac
    19561 cagcccgggc gtgccttttt cgtccttggt gttggggtca aagccgcgcg ccagcaggct
    19621 gcgcacggcg gcgtcgttgt cctgccggat ggcgcgaaag aattcgtcgc tgtgggcgtc
    19681 ggccagcgcg gcggcgctgc acaggcccag cagggcggtc aggaacaggc ggcgaagcat
    19741 ggcgctcatg gggcaaccct cgaaaacagg cgggtgaagt tggcactggt cagttcaccg
    19801 atgcgttcgg tgcgggtgcc gcgcagctcg gccagccgcg ccgccaccag gggcacgtag
    19861 gccggcgtgt tgagcttgcc gcggtgcggc accggcgcca gatagggcgc gtcggtttcg
    19921 atcagcaggc ggtcgtcggg gatcagggtc gccacctcgt gcagctcgcg cgcattcctg
    19981 aaggtgacga tgcccgacag cgagacgtag aaacccatgt cgagcgcggc gcgcgccacg
    20041 gcccgggtct cggtgaagca gtgaaacacc ccgccggcgc tgccggcgct gccgtcctcg
    20101 ccttcttcgc gcaggatggc cagggtgtcg tcggcggcgg cgcgggtgtg gatcaccagc
    20161 ggcacgcggg cccgccgcgc ggcgcggatg tgggtgcgaa agcgttcgcg ctgccagccc
    20221 atgtcggcaa tgctgcggcc gcccttgcgc tcgtccatct ggtagtaatc gaggccggtt
    20281 tcgcccaccg ccaccacgcg cggccgggtg gtggcggcca ccagctcctc gacgtccggt
    20341 tcatgcacct gctcgctgtc gggatgcacg cccaccgtgg cccagaagtt gtcgtggccg
    20401 ctggccagcg cctgcacggc ggggaactct tccatcgtgg tgcagatgca cagcgcgcgc
    20461 gtcacgcggg cggcggccat ggcggcgcgc acggcgggga tgtcacccgc cagttcggga
    20521 aaggtcaggt ggcagtggga atcggtgaac atgcggatgg ccgcgcctgc ggccaggggc
    20581 cgcatcagaa cgcggcgccg atgctagcag cccggcagga acccgggcgt tcagatggtc
    20641 tgcgtggggc ggtcggagcc cagggcggta cccaggatct gttcgatctt ggtcttgagc
    20701 tgggccgatt tttcgtcttt cgggaagcgg atgccgatgc cctgggtgcg gttgccgacc
    20761 gagcgcgcgg gcgtgatcca ggccaccttg ccggcgatcg ggaaacgctg tgggtcgtcc
    20821 ggcagggtga tgagcacgta gacgtcgtcg cccagccggt agtcgcgcgt cgaaggaatg
    20881 aagatgccgc cttcggcgaa catgggaatg taggcggcgt acagggccgc cttttccttg
    20941 atcgccagct ggatgacgct gggccggggc gtggaagccg ccgcattcgg ggtggtcgac
    21001 atggcgatgg gcgtgggcgc gacagaaggg tcagcggccg gagtttaggg cctgacgggc
    21061 gctgctgacc aggaattctg tcgccaaacc ggcatttaat gggtgttccg ccgtgcgcgc
    21121 cgactgcgcc agctgctgcc accagcgcgt cagcgccagc agcgacagcg gctggcgcgg
    21181 cagatcggcc ggcgcgaaaa agcgcggcgc cgcgcccacc tgcagcgcca gcaggtcatg
    21241 gcacagcttc tgcagccgcg ccaccgcctc cggcagcggc aaatcggcca gggcggccac
    21301 gtcgccgcgc gccagcgccg gcgccagctg cgaccagcgg cgcgcgctgt ccttgccttc
    21361 ggccagcgcc agcgcctcgg ccggtcggcc gccggcggcc tgcagcaggg cggcggcctg
    21421 cgggcccgcc agaccctggg tggccagcca ggccagggcc tcgtcggccg gcggccaggc
    21481 cagggtgtgg ctcaggcaac ggctgcggat ggtgggcagc agcaggtgcg cggcgtcggt
    21541 cgccagcacg aagcgggcgt cgcccggcgg ctcctccagc gtcttgagca gcgcgttggc
    21601 ggtgaccgcg ttcatgcgct cggccgggta caccagcacc gccttgcccc ggccacggcc
    21661 gctggtgcgc tgggcgaatt cgatcgcttc gcgcatggcg tcgacgcgga tctcgcggct
    21721 gggcttgcgc ttcttgtcgt cgatctcggc ctgcgccttc tcgggcagcg gccagccgcg
    21781 cgccagcatc tcggtttcgg gcatcagcac caccaggtcg gggtgggtgt gcacgtcgat
    21841 gccgtggcag ctcgggcaca ggccgcaggc gccgtccggc gtcggctggt cgcacagcca
    21901 ggcgcgcacc agcgccagcg ccagctcgaa ctggcccagg cccgacggcc cctgcagcag
    21961 ccaggcgtgg ccgcgctggg cccgcagcgt cgcgtgctgg cgcgcgatcc agggagccag
    22021 ggcggcgccg ctcacagcca accccgcgcc tgcacggcgc cgtggacggc acgccacacc
    22081 gcctcgcgcg gctggtcggc gtcgatgcgg gcaaagcgcg ccggctgctg gctggcgcgg
    22141 cgcgcgtagc cggcacgcac ggcctcgaag aaggcggccg gctgggcctc gaagcggtcg
    22201 ggcacgcggg tgccggccag ccgttcggcc gccaccgcgg gcggcagatc gaaccagatg
    22261 gtgaggtcag gctggaccat tccttcatgg gccaaatcgg cccctggcgc ttgcacagtc
    22321 tgcgccagac gctccaataa cgatagcgac tcccagtcga acccgcgccc gccgccctgg
    22381 taagcgaagg tggcgtcggt gaagcggtcg cacagcacca cctcgccgcg cgccagcgcc
    22441 ggaacgatca cctgggtcag atggtcgcgc cgggcggcga acatcagcag cgtttcggtc
    22501 agcgcgtcca tcgcctcgtg cagcgccagc gcgcggattt tctcggccag cggcgtaccg
    22561 ccgggttcgc gcgtgcgcac cacggtgcgc ccggcctcgc gcagcgcctg ggccagcgcc
    22621 tcgatgtggg tcgacttgcc ggccccgtcg atgccttcga agctgatgaa ccgcccgtgc
    22681 tgcgccatca ccggctcccc ccgcgctggt accgctggac ggcgcagttg tgttcgtcca
    22741 gcgtgcggct gaagtggctg ctgccgtcgc cacgcgccac gaaatacagc gcctgcgtgg
    22801 ccgccggctg caccgccgcc agcagcgccg ccttgccggg catggcgatc ggtgtgggcg
    22861 gcagaccggc gcgggtgtag gtgttgtagg gggtgtcggc gctcaggtgg ctgcgcttga
    22921 ggtcgccgtc gaacccctcg cccaggccgt agatcacgct cgggtcggtc tgcagccgca
    22981 tgccgatgcg cagccggttg atgaagacgc cggcgatctc ggcccggtcc tgggcgcggc
    23041 cggtttcctt ttcgacgatg ctggccagca ccagagcctc gtcggcgctt ttcagcggca
    23101 ggtcgggcgc gcgcgcgtcc caggcggcct gcaggcgctg ctccatcagc tgcatcgact
    23161 ggcgcaggat gtccagttcg ctggcgttgc gaaagtaatg gaaggtgtcg ggaaagaaac
    23221 gcccctcggc cgccacaccg ggccggccca gcgcctgcat gatctgcgcc ggcgtcatgt
    23281 cctgggtgtc gtggcgcagc cgcggcgcct gcgccagcgc ctgacgcaac tggcggaaag
    23341 tccagccctc cagcagcttg acggtgagca cgatgcgttc gccgcgcacc agcttgccca
    23401 gcagctcggc cggcgtcatg ccgcgcgtca gcgcgtagtc gcccggcttg atggtctgcc
    23461 cgcgcgccgc cagccggaag tagcccgcca gcagcgccgc cggggtctgc acgccgccgt
    23521 ccaccgcgcg ctgcgccgcc gtgcgcagcg aggcgccttc gggcacgctg atttccagcg
    23581 cgtcggcggc cagcggcagc ggctgctgcg tccaccacca ggccccggcg ccggccgcca
    23641 gggccagcgc cagcagcaat gccagcagtt tcagcagggt tcgcacgagt cggcgcaggg
    23701 cgtgaatggg aaccggccat gataattcag cccatgtcgg acaccgcaac cgcttccttc
    23761 accgcctcgg ccctgcacgg catcgctccc ctgccccacc tgggcgtgat ccgcgcccac
    23821 ggggaagacg cggcgcactt tctgcagggc cagctgacgc aggacgtgac ggggctgaag
    23881 ccgggccagg cgcgcctggc ggccctgtgc aaccccaagg ggcgcatgct ggccagcttt
    23941 caggtgctgc gccgcgccga cgacgagctg ctgctggtca ccagcgccga cacgctggcg
    24001 gccacgctca agcgcctgag catgttcgtg ctgcgcgcca ggctgaagct gagcgatgcc
    24061 acggccgaac tggcgttgcg cggcctgctc ggaaacgcta ccacttcagt agctgctggc
    24121 gcccatcaga caagcgctgg agcccaaaac gacccagcat tcacgatctc cctgccggcc
    24181 gccgacggcg caccgcgcgc gctctggctg gcggcggccg gcacgccgcc gccggccgga
    24241 gccgaactca gcgaggccga ctggctggcc gccgaggtgc gcgccggcgt ggcgcgcatc
    24301 acgcggccgg tggtcgaggc cttcgtgccg cagatgctca actacgaaag cgtggacggg
    24361 gtgcattttc agaaaggctg ctaccccggg caggaggtgg tggcgcgcag ccagtttcgc
    24421 ggcgccatca agcggcgcgc cttcgtcggc tgggccgacg gcgcggtgca ggcggggcag
    24481 gaagtgttca gcgccagcga gcccgagcag ccggccggcc tgatcgccca ggccgccgcc
    24541 gtggccggcg gcacggcggt gatcgccgcg ctgcggctgg acgcgctgcc caccgcgctg
    24601 gcc
//
LOCUS       DAOQTZ010000036        23881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2479_length_23881_cov_12.584823, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000036 DAOQTZ010000000
VERSION     DAOQTZ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 23881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23881
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2479_length_23881_cov_12.584823"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PKN51_06105"
     CDS             <1..859
                     /locus_tag="PKN51_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNT84708.1"
                     /translation="ISPLDRGFIFGDGIYEGISIYGRPGHAPQPFRFEQHMARLERGL
                     RETRIPNPHTADEWRQIALDLIAAYARQSSADATSDAQAAPQDWFFYFQVTRGVALRD
                     HPMLPGLTPTVFATCLPMKPPTAEQRAQGVACVTADDFRWQKAHLKSISLLGAVFARQ
                     ISVDAGALETVMFRDGHLSEAAASNVWVVSNSVVMGPPKDHLVLEGIRYGAIEELCRQ
                     EGIGFELRRIPRADVLAADELLLSSATKEVLPITTLDGQPVGSGRPGPVSQRLYAAYQ
                     RAKDALFAA"
     gene            856..1140
                     /locus_tag="PKN51_06110"
     CDS             856..1140
                     /locus_tag="PKN51_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HNT84709.1"
                     /translation="MTAPPADPRRDTLIDYPSAFPIKVMGLRQDGFVHAMTELARQHD
                     PGFDAATVELRESAKGNYLGITLTVTATSREQLDNLYRALSSHPMVKVVI"
     gene            1223..2860
                     /locus_tag="PKN51_06115"
     CDS             1223..2860
                     /locus_tag="PKN51_06115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNT84710.1"
                     /translation="MPTLDWLGRADAFGAAARVPYRLLAPISQHGDARAAADNLLIQG
                     DNLQALKALLPLHRGQVKCIFIDPPYNTQSAFEHYDDNLEHAQWLTMMLPRLQLLREL
                     LREDGSIWVTIDDHEGHYLKVLMDEVFGRGNFVSTAIWQKKYAPKSDSKYLSESHDFV
                     LGYARKLDALTLNRIEKGDKQIARYTNRDNDSRGPWKASDVLRSEARDYAIFPVTLPS
                     GRVVWPPSGTSWRYTKEKFAELIADNRIWFGVNGDARPAYKRFLSEVTDSMPATTIWS
                     HDEVGHNDEAKKEMRGLFTEDLFGTPKPERLLQRILHIATNPSDLVLDSFLGSGTTAA
                     VAHKMGRRWIGIEMGEHARTHCLPRLLKVVEGEPGGISRAVGWAGGGGFRFMQLGAPV
                     FDAAGHIHPEVRFDTLAAFVWQQETGRAHTPGDPATGACPGTPLLGIHYDLDSCPRSP
                     DGRESPISPEAPQAAAPAARAAIYLLFNGILGDKRPAGGNVLTREVLAALLALHAGSA
                     APQAPLVVYGEACRVGPARLAQAGVTFRHIPHDIRAG"
     gene            2841..3677
                     /locus_tag="PKN51_06120"
     CDS             2841..3677
                     /locus_tag="PKN51_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNT84711.1"
                     /translation="MTSGRGEGMKETASATAPYFVDESGDGVLFTARGQLALQQAEGV
                     PTFMLGLLHLPDPEALGAELVALRAQLLADPYFRHVPSMQAQARKTALAFHAKDDVAE
                     VRREVFRVLLRHDMKFFAVVRDMRAVLAYVQERNRRDDAYRYRPNDLYDQTVARLFKD
                     RLHQHERNAITFARRGNSDRTRALRAALELARQRFEAKWNAQVKSEIALASARPSESA
                     GLQAVDYLLWALQRHYARGESRFIELMWPKVSLIHAVDETRAARYGVYYTRKKPLVGA
                     AS"
     gene            3867..6557
                     /locus_tag="PKN51_06125"
     CDS             3867..6557
                     /locus_tag="PKN51_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNT84712.1"
                     /translation="MPTLALKSYQQAALDTLTAFAQAAQVGGAARAFEALAGRPYQSE
                     PFGPDVPCVCLRIPTGGGKTLLAAHAVPLLARHWRASDAPVAVWLVPSDAIRAQTLQG
                     LQTPGHPVRQVLADAYGERLRVCALEDVAQIAPPDWGRAAVVVVATIQSFRIDDTDKR
                     NVYSFAESFERHFKAAQSEQPRALAALAALPGACVTADEAAQDGRALLRGFVGQPRQS
                     LANWLALHQPIVIVDEAHNAKTDKSFTALKRLNPACLLELTATPIAARTNVLYHVSAQ
                     ELAAEHMIKLPIALAEHPEGWPQAVLATVRTRAALEAEALKDEAAGAGYVRPIALYQA
                     QNAGDAVPPEALRRHLIDELHVPPEQVVVATGSTRELDGLELAARDCPVRHVITVQAL
                     REGWDCPFAYVLCSLQKLASATAVEQLLGRVLRMPYATRRGREPLNRAWAHVCEAEFS
                     SAAHALADRLIQGMGFEALDVASMLAPAASLPLFEENQAPAPVQQAPVATDFESIAPT
                     DALAALPGVAVRTIAGAPRVVVSGHIGAELEGQLLAGVRGAKKQDAVRQQIARHNALV
                     AAQTAPAARGEHFAPVPPLGYREPSQSELWPLEREALLEAVDIDLLTPEAIELPGFQV
                     VEQGRAFEIYLKDEQVRLRPAEAGQIALDYGVSGIGAQDLVRWLEQSLYASLPEFTPA
                     QRQPWFAAVVNRLLHEQRVPIETLAQARFTLARALQAKAADLRDAAAKARFHQLVLDG
                     GWQVEPDWARPHVFGPATYPAPAGSRYGGRYEFRKHYYPVIADLKSEGEEFACAQLID
                     RHPKVKHWVRNLDAAPAGFWLPTSRRRFFADFVAELVDGRIALLEYKGAHLASDPAEI
                     EKRQIGELWARRSGGRAVFGWLMKAQDGLGPAQQLDAVLA"
     gene            6554..7252
                     /gene="lipB"
                     /locus_tag="PKN51_06130"
     CDS             6554..7252
                     /gene="lipB"
                     /locus_tag="PKN51_06130"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNT84713.1"
                     /translation="MTLEVRFLGRVDYAPTFATMKEFATRRPSGGREQLWICEHLPVF
                     TLGLAGRPEHLLAPGDIPVVPTDRGGQVTYHGPGQVVAYPLIDLAARGYFVKEYVHRL
                     EEAVIRTLAQWGVTGHRVAGAPGIYVRLADPFGHAPLAQRPQRRAAGEPAPPPDFAGL
                     GKIAALGIKVSNHRSYHGVALNVAMDLSPFDRINPCGYAGLRTVDLRTLGVTATWDEV
                     AHALATHLERLLQA"
     gene            7266..8159
                     /locus_tag="PKN51_06135"
     CDS             7266..8159
                     /locus_tag="PKN51_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT84714.1"
                     /translation="MTTAARPRHTTANLLALATIALWASLATLGASLAHVPPFLLTGL
                     GLLAGSVIALPLVRFDLRRLAVTPRALALGVYGLFGYHFLLFTALQLAPPVEANLVNY
                     LWPLGIVLLAPLLLPGVRLAPAHLLAALAGLAGAAIAILGRGAGAESGGAGHPLAALG
                     FACAAGAALIWSSYSVLSKRLASTRHAFPTAAIGTFGFVSALLSLLCHALLEPAVSLS
                     ARDLGLIAVLGLGPLGGAFFLWDAALKLGDARQIGVLSYLTPLLSTLTLLWATGRPLT
                     GSIVLATALILGAAVIGTRAR"
     gene            complement(8482..10227)
                     /gene="ptsP"
                     /locus_tag="PKN51_06140"
     CDS             complement(8482..10227)
                     /gene="ptsP"
                     /locus_tag="PKN51_06140"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNT84715.1"
                     /translation="MSFSIHGISVTRGIAIGRAVLVASSLVDVTHYFIAPEQVEAEIA
                     RLRAARNAVVEELQRMQADMPRDLPPELGAMLDVHLMLLQDEALTSEIRRWITERLYN
                     AEWALASQLEVLIRQFDEMDDPYLRERKADLEQVVERVLRHLKGGPAPIAAPAPRAGR
                     QQELGLGDIDVPLVLVAHDLSPADMLQFRKSVFAGFVTDVGGKTSHTAIVARSMDIPA
                     VVGARSASHLVRQDDWVIIDGDAGVMIVDPSPIILAEYGFKQRQGELERGRLARLKHT
                     PAVTLDGQRIELLANIEQPGDTAAALAAGAVGVGLFRTEFLFMGRQGKLPDEQEQYDA
                     YRAAVEGMHGLPVTIRSIDIGADKPLDDRRPDSHLNPALGLRAIRWSLAEPVMFLTQL
                     RAILRAAAHGQVQLLIPMLAHASEIRQTFELIERARAQLDQRGQTHGPVKVGAMIEIP
                     AAALSLRLFLRHFDFLSIGTNDLIQYTLAIDRTDESVAHLYDQLHPAVLRLLADTLAE
                     GARQGKPVSVCGEMAGDTSMTRLLLGLGLRSFSMHPSQILAVKQEILRADATKLAPWA
                     QAVLDSDEPAALLAP"
     gene            complement(10224..10493)
                     /locus_tag="PKN51_06145"
     CDS             complement(10224..10493)
                     /locus_tag="PKN51_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNT84716.1"
                     /translation="MIKTDITISNKLGLHARAAAKLTKLAAAYPCEVWMSKGERRINA
                     KSIMGVMMLAAGIGSVVTLETDGAQEQEAMDALRALIDDKFGEGQ"
     gene            complement(10504..10912)
                     /locus_tag="PKN51_06150"
                     /pseudo
     CDS             complement(10504..10912)
                     /locus_tag="PKN51_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(10924..12183)
                     /locus_tag="PKN51_06155"
     CDS             complement(10924..12183)
                     /locus_tag="PKN51_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT84717.1"
                     /translation="MSAGAELLPRRTAVLVFLAFAGAYFLSALLRAVTATLSPVLTAD
                     FALHARDLGLLAGGYFLGFAAMQLPLGTWLDRYGPRRVSVALLAVAVLGCAVFALAQG
                     FAGLLAARVLMGMGVAAGLMAPLTGYRRWFDAATQLRANAWMLMTGSLGMLASTLPVQ
                     WLLPLLGWRPLFWGLALLGLLAMAVIALRVPGWRPPDATNSIADGADRAGAGGWNGSY
                     AQIVRQAEFRRAWPLGMVSFGGMVAIQSLWAGPWLTRVVGRSPLEAATGLFWINASML
                     LTFWAWGLLLPRLTARGIGIDAIVARVQPLSFVALAALVAAGPAVGGWGVALLALYCV
                     LSTPSAQVQPVLALAYPPQVAGRALSAFNLVIFVGIFLVQWGIGLGADALAALGWSEA
                     DALRGALGAFGLLSAACWLHHLRAGRR"
     gene            complement(12180..12719)
                     /locus_tag="PKN51_06160"
     CDS             complement(12180..12719)
                     /locus_tag="PKN51_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84718.1"
                     /translation="MLLHRAALVLAVALGAAACTPTLDWREVRLAPSPAIALLPCKPD
                     RTTRDVPLGGVPTALSVAGCDAGGATFALMTAQLPAGRDPDAVLAGWQQATLAHMRAQ
                     GTPERRPFTPPGARALPHAQRLTARGQRPDGRPVMAQAVWSAQAAAGGGTELLHAVVY
                     AERLRPEAADAFFDAIRWP"
     gene            12760..13998
                     /locus_tag="PKN51_06165"
     CDS             12760..13998
                     /locus_tag="PKN51_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNT84719.1"
                     /translation="MNSLDGIRVLDLSRVLAGPWCTQTLADLGADVIKIERPGSGDDT
                     RSWGPPFLRGDDGTESREAAYYLGANRNKRSVTCDIAQPEGQQLIRELVAHCDVFIEN
                     FKVGDMARYGLDYAALSALNPRLVYCSLTGFGQTGPYAPRAGYDYIIQGMGGLMSVTG
                     ERDDQGGGPQKVGVAVADLFTGMYATVGILAALRHAERTGAGQHLDMALLDAQVAMLA
                     NLGANHLVAGTVPGRMGNAHVNIVPYQVFEVAPGDGGDKDFIILAVGNDGQFAKFCAV
                     AGQPELARDERFATNAARVRHRAVLVPLLEAILKARAKADWLAALEVAGVPCGAINRL
                     DEVFADPQVRSRGMVDHWTHPLAAELRLVASPLKLSATPVRTDRPPPLLGQHTDEVLT
                     ELLGADTKRLAALRAARVIQ"
     gene            14114..14845
                     /locus_tag="PKN51_06170"
     CDS             14114..14845
                     /locus_tag="PKN51_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT84720.1"
                     /translation="MANYLLVHGAWGGAWYWRPVQHALIRAGHNAHAVTLTGLGERAH
                     LLSPAITLGTHIADVRQALAAEELSDCILVVHSYAGMLGTAVADREPPGTLRHLVYVD
                     AVVPKPGESWSGTHASGTRRERLHAAEISPTHSFPAPDPGVFGLSAADHAWVARRQVP
                     HPGHTYLEPLEFDPRRVARVPRTFIDCTQPKLATIDVIRQRVRDPAFWDGAWLPGSRI
                     VEMATGHDPMISETEAMTRLLLELA"
     gene            14960..15430
                     /locus_tag="PKN51_06175"
     CDS             14960..15430
                     /locus_tag="PKN51_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT84721.1"
                     /translation="MALASLLLGASLLSACGNDAAPPSTFVLLDGSSQTTADLKGKVT
                     LVNFWATSCTTCVAEMPDIVATYDKFKARGYETIAVAMQYDPPSYVVNFAETRKLPFK
                     VAIDNTGAVAKAWGDVKLTPTTYVVNKQGEIVKRYVGAPDFSELHQLIEKLLAA"
     gene            complement(15727..16191)
                     /locus_tag="PKN51_06180"
     CDS             complement(15727..16191)
                     /locus_tag="PKN51_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNT84722.1"
                     /translation="MKKKLSVLAFTLAIAGAAAPTFAQQQYAKAEDAVHYRRGAFTLL
                     GYHFGSLGAMVQGKVPFNSAQAAIDGDVIAIASKLPFHAFVEGSDKGQTGAKPEIWKE
                     QAKFTDLRDKMQAEVAKVSAAAKSGNLDNLKAVFGAAGQSCKNCHDNYRRRL"
     gene            16410..17084
                     /locus_tag="PKN51_06185"
     CDS             16410..17084
                     /locus_tag="PKN51_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNT84723.1"
                     /translation="MHTVRIWDLPTRLFHWVLFVCVVALVVTAKVGGNAMNLHLRLGY
                     VVFTLLLFRLLWGFVGGHWSRFVSFFPTPGRLTRYLGGRATTADTAGHNPLGALSVLA
                     MLLVLGAQVGSGLFSDDEIAFSGPLTALVSNATVGRLTEWHSHWGQYLVIGLVLLHVL
                     AIVVYRLRRQNLVGPMVVGDKTLPEPVTAARDSAGTRLLALALLGVAAALVYAVVQLG
                     SRGALG"
     gene            17154..17633
                     /locus_tag="PKN51_06190"
     CDS             17154..17633
                     /locus_tag="PKN51_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT84724.1"
                     /translation="MHLAPAREPAHYDAARALFREYADQLGVDLCFQGFEAELAGLPG
                     DYQEPGGTLLLAWVDGELAGCCALRPIEDVDYANAAEMKRLFVRKAFRGFGLGRQLAE
                     AVLEAAVRLGYDSVLLDTLDDMEAARALYEDLGFVEIPPYYHNPLPGAHYLKVELRV"
     gene            complement(17733..18242)
                     /locus_tag="PKN51_06195"
     CDS             complement(17733..18242)
                     /locus_tag="PKN51_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNT84725.1"
                     /translation="MIKVGDALPAVTLSEYIEVEGNGCSIGPNPVEVQKAAAGKTIAV
                     FAVPGAFTPTCSAKHVPSYLQHAAALKAAGVDEIWCLSVNDAFVMGAWAREQKTAGQI
                     RMLADGDAAFAQATGLTLDLHGKGLGLRSNRYSMLVKDGKVAALNVEAPGKFEVSDGA
                     TLLAQLRAA"
     gene            complement(18386..18655)
                     /gene="rpsQ"
                     /locus_tag="PKN51_06200"
     CDS             complement(18386..18655)
                     /gene="rpsQ"
                     /locus_tag="PKN51_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNT84726.1"
                     /translation="MTESQPSLKRTLIGKVVSDKRAKTVTVLVERRVKHPIYDKIVMR
                     SSKYHAHDENGQYKLGDTIEITESRPLSKTKNWVATRLVQKAAQV"
     gene            complement(18665..18862)
                     /gene="rpmC"
                     /locus_tag="PKN51_06205"
     CDS             complement(18665..18862)
                     /gene="rpmC"
                     /locus_tag="PKN51_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNT84727.1"
                     /translation="MKTAELRQKDVAGLQAEVKELQKAHFNLRMQKGTQQLSNTSQLK
                     ATRRAIARAKTLIVEKQAAAK"
     gene            complement(18879..19295)
                     /gene="rplP"
                     /locus_tag="PKN51_06210"
     CDS             complement(18879..19295)
                     /gene="rplP"
                     /locus_tag="PKN51_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNT84728.1"
                     /translation="MLQPARRKYRKEHKGRNTGIATRGNTVAFGDFGLKSTDRGRLTA
                     RQIESARRAISRHVKRGGRIWIRVFPDKPISTKPAEVRMGNGKGNPEYYVAEIQPGKV
                     LYEIVGVPEELAREAFALAAAKLPLRTTFVARMIGQ"
     gene            complement(19299..20195)
                     /gene="rpsC"
                     /locus_tag="PKN51_06215"
     CDS             complement(19299..20195)
                     /gene="rpsC"
                     /locus_tag="PKN51_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNT84729.1"
                     /translation="MGQKIHPTGFRLSVSRNWASRWYASNRDFAGMLAEDIKVREYLK
                     AKLKNAAVSRVLIERPAKNARITIYSARPGVVIGRKGEDIEKLKKELADQLGVPVAVN
                     IEEVRKPEVDAQLIADSITQQLEKRIQFRRAMKRAMQNAMRLGAQGIKIMSSGRLNGI
                     EIARTEWYREGRVPLHTLRADIDYGFSEAHTTYGAIGVKVWVYKGDTLGRNDAPSLDS
                     TPRPEGEERRGPRGPRRDGRGGPGGDRRGGPRRDARGGGSGAPADGSDKPAEGQGAEA
                     KPAVKRVRKAAPAAAADGAQGE"
     gene            complement(20215..20553)
                     /gene="rplV"
                     /locus_tag="PKN51_06220"
     CDS             complement(20215..20553)
                     /gene="rplV"
                     /locus_tag="PKN51_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNT84730.1"
                     /translation="MAATETRAVLRGVRLSVDKGRLVADLIRGKKVDQALNILNFTQK
                     KAAVIVKKVLESAIANAEHNDGADIDELRVKTIYVEQGMTLKRSAARAKGRGARLSKP
                     TCHVYVTVGN"
     gene            complement(20563..20838)
                     /gene="rpsS"
                     /locus_tag="PKN51_06225"
     CDS             complement(20563..20838)
                     /gene="rpsS"
                     /locus_tag="PKN51_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNT84731.1"
                     /translation="MTRSLKKGPFVDHHLLAKVDKAVASKDKKPIKTWSRRSMILPEF
                     IGLTIAVHNGKQHVPVYVTDQMIGHKLGEFALTRTFKGHAADKKAKK"
     gene            complement(20851..21675)
                     /gene="rplB"
                     /locus_tag="PKN51_06230"
     CDS             complement(20851..21675)
                     /gene="rplB"
                     /locus_tag="PKN51_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNT84732.1"
                     /translation="MAVIKIKPTSPGRRGQVKISRDHLHKGEGYAPLLEPQFQKAGRN
                     NNGHITTRHKGGGHRHHYRVIDFRRNKDGIAAKVERIEYDPNRSAHIALVCYADGERR
                     YIIAPRGVEVGSQLMSGSEAPIRAGNTLPIRNIPVGSTIHCIELKPGAGAQIARSAGT
                     SATLLAREGTYAQVRMRSGEVRRVHIECRATIGEVANEEHSLRQFGKAGVKRWMGIRP
                     TVRGIVMNPVDHPMGGGEGRSKSGRHPVDPWGNLTKGYRTRSNKRTQNMIVSRRKK"
     gene            complement(21679..22014)
                     /gene="rplW"
                     /locus_tag="PKN51_06235"
     CDS             complement(21679..22014)
                     /gene="rplW"
                     /locus_tag="PKN51_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNT84733.1"
                     /translation="MSRINPTPKERQFDEGRLMQVLVAPVISEKATMVAEKSNAVTFK
                     VLRSATKPEIKAAVELLFNVKVRGVSVANIKGKTKRFGRSIGRRDHVRKAYVLLQEGQ
                     ELNLSGEAA"
     gene            complement(22011..22631)
                     /gene="rplD"
                     /locus_tag="PKN51_06240"
     CDS             complement(22011..22631)
                     /gene="rplD"
                     /locus_tag="PKN51_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNT84734.1"
                     /translation="MQLELLNDQGQAASKVDAPETLFGRDYNEALVHQLVVAYQANAR
                     QGTRAQKDRAQVKHSTKKPFRQKGTGNARAGMTSSPLWRGGGRIFPNSPFENFSQKLN
                     KKMYRAGMASILSQLVRDGRLAVVESIKLDSPKTKQLAQKFKDMKLDSVLVIADEVDE
                     NLYLASRNLGNVLVVEPRYADPLSLVHYKKVLVTKGAIDQLKEMFA"
     gene            complement(22631..23311)
                     /gene="rplC"
                     /locus_tag="PKN51_06245"
     CDS             complement(22631..23311)
                     /gene="rplC"
                     /locus_tag="PKN51_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNT84735.1"
                     /translation="MSQSNRLGLLGRKVGMMRLFTDDGEAVPVTVVDVSNNRVTQVKT
                     QENDGYVALQVTFGSRKASRVTKPEAGHLAKAGVEAGEITQEFRVDGNVAGKYAAGTA
                     LPVAEVFAAGQKVDVQGTSIGKGYAGTIKRHNFSSQRASHGNSRSHNVPGSIGMAQDP
                     GRIFPGKKMTGHMGDETVTTQNLDLIRIDETRQLLLIKGAVPGARGGFVTVRPAVKAK
                     AAANEGAN"
     gene            complement(23497..23811)
                     /gene="rpsJ"
                     /locus_tag="PKN51_06250"
     CDS             complement(23497..23811)
                     /gene="rpsJ"
                     /locus_tag="PKN51_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNT84736.1"
                     /translation="MATKQKIRIRLKAFDYKLIDQSAAEIVDTAKRTGAVVKGPVPLP
                     TRMKRFDILRSPHVNKSSRDQLEIRTHQRLMDIVDPTDKTVDALMKLDLPAGVDVEIK
                     LQ"
BASE COUNT         3485 a         8272 c         8316 g         3808 t
ORIGIN      
        1 catcagcccg ctggaccgcg gcttcatctt cggcgacggc atctacgagg gcatctccat
       61 ctacggccgg cccggccacg cgccgcagcc gtttcgcttc gagcagcaca tggcgcggct
      121 cgaacgcggc ctgcgcgaaa cccgcatccc caacccccac acggccgacg aatggcgcca
      181 gatcgctctt gatctgatag ctgcttacgc ccgccagtca agcgccgacg ccacaagcga
      241 tgcccaagcc gcgccccagg actggttctt ctacttccag gtcacgcgcg gcgtggcgct
      301 gcgcgaccac cccatgctgc cggggctgac acccaccgtg ttcgccacct gcctgcccat
      361 gaagccgccc accgccgagc agcgcgcgca gggcgtggcc tgcgtcaccg ccgacgactt
      421 ccgctggcag aaggcgcacc tcaaaagcat cagcctgctg ggcgcggtgt tcgcgcgcca
      481 gatcagcgtg gacgccggcg cgctggaaac cgtgatgttc cgcgacggcc acctgagcga
      541 agccgcggcc agcaacgtgt gggtggtcag caacagcgtg gtcatgggcc cgcccaagga
      601 ccacctggtg ctggaaggca tccgctacgg cgccatcgag gagctgtgcc ggcaggaggg
      661 catcggcttc gagctgcgcc gcatcccgcg cgccgacgtg ctggccgccg acgagctgct
      721 gctgtcctcg gccaccaagg aggtgctgcc catcaccacg ctcgacggcc agcccgtcgg
      781 cagcggccgg cccggccccg tcagccagcg gctgtacgcc gcctaccagc gcgccaagga
      841 cgccctgttc gccgcatgac cgccccgccc gccgacccgc gccgcgacac gctgatcgac
      901 taccccagcg ccttccccat caaggtcatg ggcctgcgcc aggacggctt cgtgcacgcc
      961 atgaccgagc tggcgcgcca gcacgacccc ggcttcgacg ccgccaccgt cgagctgcgc
     1021 gaaagcgcca agggcaacta cctgggcatc accctcaccg tcaccgccac cagccgcgag
     1081 cagctcgaca atctgtaccg cgccctcagc agccacccca tggtcaaggt ggtgatctag
     1141 tcaaaaccgc ctctggcgcc cgtctggcat gcgccagcag ctatgatttt tgactgttga
     1201 cacccccccc cgccgctgac ccatgcccac cctcgactgg cttggccgcg cggacgcctt
     1261 tggcgccgcc gcccgcgtgc cctatcggct gctggccccc atcagccagc acggcgacgc
     1321 gcgcgccgcc gccgacaacc tgctgatcca gggtgacaac ctgcaggcgc tcaaggccct
     1381 gctgccgctg catcgcgggc aggtcaagtg catcttcatt gacccgccct acaacaccca
     1441 gagcgcgttc gagcactacg acgacaacct ggagcacgcc cagtggctga ccatgatgct
     1501 gccgcgcctg caactgctgc gcgagctgct gcgtgaggac gggtcgatct gggtcaccat
     1561 cgacgaccac gaggggcatt acctcaaggt gctgatggat gaggtgtttg ggcgggggaa
     1621 ttttgtgagc accgcgattt ggcagaaaaa atacgcgcca aagtccgact caaagtacct
     1681 ttccgaatca cacgatttcg tgctgggtta cgccaggaag ctggatgcgt tgacgctgaa
     1741 tcgaatcgag aaaggtgaca agcaaatcgc ccgctacacc aatcgcgaca acgattcgcg
     1801 cgggccttgg aaagccagcg atgtcctgcg cagcgaggcg cgtgattacg ccattttccc
     1861 cgtcacgttg ccatcagggc gcgtggtgtg gccaccttca gggacgagct ggcgctacac
     1921 gaaagagaag ttcgctgaac tcatcgcgga caaccgcatc tggtttggcg tcaatggcga
     1981 tgcgcggcca gcctacaaac gcttcctcag cgaggtgacc gactccatgc cggcaaccac
     2041 catttggtct cacgacgaag tcggacacaa cgacgaggcc aagaaggaaa tgcgcggctt
     2101 gttcactgaa gacctgttcg gcaccccaaa acccgaacgc ctcctccagc gcatcctcca
     2161 catcgccacg aaccccagcg acctcgtcct cgacagcttc ctcggctcgg gcaccacggc
     2221 ggcggtggcg cacaagatgg ggcggcgctg gatcggcatc gagatgggcg agcacgcgcg
     2281 cacgcactgc ctgccgcggc tgctgaaagt cgtcgagggc gaaccaggcg gcatcagccg
     2341 cgccgtgggc tgggccggcg gcggcggctt tcgcttcatg caactgggcg cgccggtgtt
     2401 cgacgccgcc ggccacatcc accccgaggt gcgcttcgac accctggccg ccttcgtctg
     2461 gcagcaggaa accggccgcg cccacacccc cggcgacccc gccaccggcg cctgccccgg
     2521 cacgccgctg ctcggcatcc actacgattt ggatagctgc ccgcgctcgc cagacgggcg
     2581 cgaaagccca atttcacccg aagcgccgca ggctgccgcg cccgctgcac gcgccgccat
     2641 ctacctgctg ttcaacggca tcctgggcga caagcgcccg gccggcggca acgtgctgac
     2701 gcgcgaggtg ctggccgcgc tgctggcgct gcacgcgggc agcgccgcgc cgcaggcgcc
     2761 cctggtggtg tacggcgaag cctgccgcgt cggccccgcg cggctggcgc aggcgggcgt
     2821 gacgttccgc cacattccgc atgacatccg ggcggggtga gggcatgaag gaaaccgcgt
     2881 ccgccacggc gccttacttt gtcgacgaat cgggcgatgg cgtgctgttc acggcacgcg
     2941 gccaactggc ccttcagcag gccgagggcg tgccgacctt catgcttggc ctgctgcacc
     3001 tgcccgaccc cgaagcactg ggcgcggagc tggtggcact gcgggcgcag ttgctggccg
     3061 acccgtactt tcgccacgtg ccgtcgatgc aggcgcaggc gcgcaagacc gccctggcgt
     3121 ttcacgccaa ggacgacgtg gcggaagtgc ggcgcgaggt gtttcgcgtg ctgctgcgcc
     3181 acgacatgaa gttctttgcc gtggtgcgcg acatgcgcgc cgtgctggcg tatgtgcaag
     3241 agcgcaatcg gcgcgatgac gcctatcgct accgacccaa tgacctgtat gaccagacgg
     3301 tggcgcggct gttcaaggac cgcctgcacc agcacgagcg caatgccatc acctttgcgc
     3361 ggcgcggcaa cagcgatcgc acgcgggcac tgcgggcggc gcttgagctg gcgcgccagc
     3421 gttttgaagc caaatggaac gcgcaggtga agtcagagat cgcgctcgcc agcgcccgcc
     3481 ccagcgaatc ggccggcctg caggcggtgg actacctgct gtgggcgctg caacgtcact
     3541 acgcgcgagg cgagagccgc ttcatcgaat tgatgtggcc caaggtgagc ctgatccacg
     3601 cggtggatga aacccgggct gcccgctacg gcgtgtacta caccagaaaa aagccgctcg
     3661 tgggagcggc ttcgtgaagg ggggtgggaa tataggattg ctgcctccgg gttgccccgg
     3721 cgggtggcac tcacacggca tgaagccgac attcgttccc actgcgggca attttagttc
     3781 aattgcacaa ggtgtggata acgggtgcac accctgttaa cccccgccgg caaccctgca
     3841 cataccgcct gctttcgatt cactccatgc ccaccctcgc cctcaagtcc taccagcaag
     3901 ccgccctgga cacgctgacc gcctttgccc aggcggcgca ggtggggggc gcggcacggg
     3961 cgttcgaggc gctggcgggg cggccgtacc agagcgagcc gttcgggccc gacgtgccct
     4021 gcgtgtgcct gcgcatcccc accggcggcg gcaagacgct gctggcggcg cacgccgtgc
     4081 cgctgctggc gcggcactgg cgcgccagcg acgcgccagt ggcggtgtgg ctggtgccca
     4141 gcgacgccat ccgggcgcaa accctccagg gtctgcaaac gcccggccac ccggtgcgcc
     4201 aggtgctggc cgacgcctat ggcgagcgcc tgcgcgtgtg cgcgctggag gacgtggcgc
     4261 agatcgcccc gcccgactgg ggccgcgccg cggtggtggt ggtggccacg atccagagct
     4321 ttcgcatcga cgacacggac aagcgcaacg tctacagctt tgccgagtcg ttcgagcgcc
     4381 acttcaaggc cgcccagagc gagcagccgc gcgcgctggc ggcgctggcc gcgctgccgg
     4441 gcgcctgcgt gacggccgac gaggcggcgc aggacggccg cgccctgctg cgcggctttg
     4501 tcggccagcc gcgccagagc ctggccaact ggctggcgct gcaccagccg atcgtgatcg
     4561 tcgacgaggc gcacaacgcc aagaccgaca agagcttcac ggcgctcaag cgcctgaacc
     4621 cggcctgcct gctggagctg acggccacgc cgatcgcggc gcgcaccaac gtgctctacc
     4681 acgtgagcgc gcaggagctg gcggccgagc acatgatcaa gctgccgatc gcgctggccg
     4741 agcaccccga gggctggccg caggcggtgc tggcgacggt gcgcacgcgc gccgcgctgg
     4801 aggccgaggc gctgaaggac gaggccgccg gcgccggcta cgtgcggccg atcgcgctgt
     4861 accaggcgca gaacgcgggc gacgccgtgc cgcccgaggc gctgcgccgg cacctgatcg
     4921 acgagctgca cgtgccgccc gagcaggtgg tggtggccac cggcagcacg cgcgagctgg
     4981 acgggctgga gctggccgcg cgcgactgcc cggtgcgcca cgtcatcacc gtgcaggcgc
     5041 tgcgcgaagg ctgggactgc ccgtttgcct atgtgctgtg ctcgctgcaa aagctggcca
     5101 gcgcgacggc ggtggagcag ttgctgggcc gcgtgctgcg catgccttac gccacgcggc
     5161 gcgggcgcga gccgctcaac cgcgcctggg cgcatgtgtg cgaggccgag ttttccagcg
     5221 cggcgcacgc gctggccgac cggctgatcc agggcatggg cttcgaggcg ctggacgtgg
     5281 cctcgatgct ggcacccgcc gcatcgctgc cgttgtttga agaaaatcag gccccagcgc
     5341 ccgtccagca agcaccagta gctactgatt ttgaatcgat cgcaccgacc gacgcactgg
     5401 cggcgctgcc cggcgtggcg gtgcggacca tcgccggcgc gccgcgcgtg gtggtcagcg
     5461 gccacatcgg cgccgagctg gagggccagt tgctggccgg cgtgcgcggc gccaagaagc
     5521 aggacgccgt gcgccagcag atcgcgcggc acaacgcgct ggtggcggcg caaacggccc
     5581 cggccgcgcg cggcgagcac tttgcgccgg tgcccccgct ggggtaccgg gaaccttccc
     5641 agtccgagct gtggccgctg gagcgcgagg cgctgctgga ggcggtggat atcgacctgc
     5701 tgacgcccga ggcgatcgag ctgcccggct ttcaggtggt cgagcaaggc cgcgcgttcg
     5761 agatctacct gaaggacgaa caggtcaggc tgcgcccggc cgaggccggc cagatcgcgc
     5821 tggactatgg cgtcagcggc atcggcgcgc aggacctggt gcgctggctg gagcagtcgc
     5881 tgtacgccag cctgcccgag ttcacgccgg cgcagcgcca gccgtggttt gccgccgtgg
     5941 tcaaccgcct gctgcacgag cagcgcgtgc cgatcgagac gctggcgcag gcgcgcttca
     6001 cgctggcgcg ggcgctgcag gccaaggccg ccgacctgcg cgacgcggcg gccaaggcgc
     6061 ggttccatca attggtgctg gacggcggct ggcaggtcga gcccgactgg gcgcgcccgc
     6121 acgtgttcgg gcccgcgacc taccccgcgc cggccggcag ccgctacggc gggcgctacg
     6181 aatttcgcaa gcactactac ccggtgatcg ccgacctgaa aagcgagggc gaggaatttg
     6241 cctgcgcgca actgatcgac cgccacccca aggtgaagca ctgggtgcgc aacctggacg
     6301 cggcgcccgc gggcttctgg ctgccgacct cgcgccggcg cttctttgcc gacttcgtgg
     6361 ccgagctggt ggacgggcgc atcgccctgc tcgaatacaa gggcgcgcac ctggccagcg
     6421 acccggccga gatcgaaaag cgccagatcg gcgagctgtg ggcgcgccgc agcggcggcc
     6481 gcgccgtgtt cggctggctg atgaaggcgc aggacggcct gggcccggcg cagcaactgg
     6541 acgcggtgct ggcatgacgc tggaggtgcg ctttctgggc cgcgtggact acgcgcccac
     6601 cttcgccacc atgaaggaat tcgcgacccg gcgcccgtct ggcgggcgcg agcagctatg
     6661 gatttgcgag catctgccgg tgttcacgct gggcctggcg gggcggcccg agcacctgct
     6721 ggcgccgggc gacatccccg tcgtgccgac cgaccgcggc gggcaggtga cctaccacgg
     6781 gccggggcag gtggtggcgt acccgctgat cgacctcgcg gcgcgcggct acttcgtcaa
     6841 ggagtacgtg caccggctgg aagaggcggt gatccgcacg ctggcgcagt ggggcgtcac
     6901 cggccaccgg gtggcggggg cgccgggcat ctacgtgcgg ctggccgacc cgttcggcca
     6961 cgcgccgctg gcgcagcgcc cgcagcggcg cgcagcgggc gagcccgcgc caccgcccga
     7021 ctttgccggc ctgggcaaga tcgccgccct gggcatcaag gtcagcaacc accgcagcta
     7081 ccacggcgtg gcgctgaacg tggcgatgga cctgtcgccc ttcgaccgca tcaacccctg
     7141 cggctacgcc gggctgcgca cggtcgatct gcgcacgctg ggcgtgaccg ccacctggga
     7201 cgaggtggcg cacgccctgg ccacccacct ggagcgcctg cttcaggcct gaacgcccat
     7261 tgcccatgac caccgccgcc cgcccccgcc acaccaccgc caatctgctg gcgctggcca
     7321 ccattgccct gtgggcttcg ctggccacgc tgggcgcgtc gctggcgcat gtgccgccgt
     7381 ttctgctgac cgggctgggg ctgctggcgg gcagcgtgat cgcgctgccg ctggtgcgct
     7441 tcgacctgcg ccggctggcg gtgacgccgc gcgcgctggc gctgggggtg tacgggctgt
     7501 tcggctacca cttcctgctg ttcacggcgc tgcagctggc accgccggtg gaggccaacc
     7561 tggtcaacta cctgtggccg ctgggcatcg tgctgctggc gccgctgctg ctgccgggcg
     7621 tgcggctggc gccggcgcac ctgctggcgg cgctggcggg gctggcgggc gcggcgatcg
     7681 ccatcctggg gcgcggcgcg ggcgccgaat cgggcggcgc cggccacccg ctggcagcac
     7741 tgggctttgc ctgcgcggcg ggcgcggcgc tgatctggtc gagctattcg gtgctgagca
     7801 agcgactggc cagcacgcgc catgcgtttc ccaccgccgc catcggcacc ttcggcttcg
     7861 tgtcggcgct gctgtcgctg ctgtgccatg cgctgctgga gccggcggtc agcctgagcg
     7921 cgcgcgatct gggtctgatc gccgtgctgg gcctggggcc gctgggcggc gccttctttc
     7981 tgtgggacgc ggcgctgaag ctgggcgacg cgcgccagat cggcgtgctg agctacctga
     8041 cgccgctgct gtcgaccctg accctgctgt gggccaccgg gcggccgctg acgggcagca
     8101 tcgtgctggc gacggcgctg atcctgggag cggcggtgat cggcacgcgg gcgcgctgat
     8161 gtgggttcaa cctggaaacg gcagttggac gcgcccgcct gtgccgtgat cggcgtgcca
     8221 ggcaagacgc gacgacgccg cgggctcatg cccgcaggga ggagcaacgc cgcatggcgc
     8281 ggcgagcgcg gtgcgggtgg gtgcgcagcg cgctcaccca ggaggtgaag gccatcgcag
     8341 ccgccagacc ccgcatcgat gcggtctgcc cgaagttgac cagcggtcaa ctgccgtttt
     8401 taggttcaaa aattgtagct gccggcgctt gccccgcctg cgtcagggcc acaaaaggcc
     8461 atgaatcgag cggcggcgcc gtcagggcgc cagcagcgcg gccggttcgt cgctgtcgag
     8521 caccgcctgc gcccacggcg ccagcttggt ggcgtcggcg cgaaggattt cctgcttgac
     8581 ggcgaggatc tgcgaggggt gcatggaaaa gctgcgcagc cccaggccga gcagcaggcg
     8641 cgtcatgctg gtgtcgccgg ccatttcgcc gcacacgctg accggcttgc cctggcgcgc
     8701 gccctcggcc agggtgtcgg ccagcaggcg cagcaccgcc gggtgcagct ggtcgtacag
     8761 gtgggccacc gattcgtcgg tgcggtcgat ggccagggtg tactggatca ggtcgttggt
     8821 gccgatggac aggaagtcga agtggcgcag gaacagccgc agcgacagcg cggcggccgg
     8881 gatctcgatc atggcgccga ccttgaccgg cccgtgggtc tggccgcgct ggtcgagctg
     8941 ggcgcgcgcg cgctcgatca gctcgaaggt ctggcggatc tcgctggcgt gcgccagcat
     9001 ggggatcagc agctgcacct ggccgtgcgc ggcggcgcgc aggatggcgc gcagctgggt
     9061 caggaacatc accggctcgg ccaggctcca gcggatggcg cgcaggccca gcgccgggtt
     9121 gaggtggctg tcggggcgac ggtcgtccag cggcttgtcg gcgccgatgt cgatgctgcg
     9181 gatggtcacc ggcaggccgt gcatgccttc gacggcggcg cggtaggcgt cgtactgctc
     9241 ctgctcgtcg ggcagcttgc cctggcggcc catgaacagg aattcggtgc ggaacagccc
     9301 cacgccgacg gcgccggcgg ccagcgcggc ggcggtgtcg ccgggttgct cgatgttggc
     9361 cagcagctcg atgcgctggc cgtccagggt gaccgcgggg gtgtgcttca ggcgtgccag
     9421 gcggccgcgt tcgagctcgc cctggcgctg cttgaagccg tattcggcca ggatgatggg
     9481 cgaggggtcg acgatcatca ccccggcgtc gccgtcgatg atgacccagt cgtcctgccg
     9541 caccaggtgg ctggccgagc gcgcgccgac caccgccggg atgtccatgc tgcgcgccac
     9601 gatggcggtg tggctggtct tgccgccgac gtcggtgacg aagccggcga acacgctctt
     9661 gcggaactgc agcatgtcgg ccggcgacag gtcgtgcgcc accagcacca gcggcacgtc
     9721 gatgtcgccc aggcccagct cctgctggcg accggcgcgc ggcgccggcg cggcgatcgg
     9781 ggcggggccg cccttgaggt ggcgcagcac gcgctcgacc acctgctcga ggtcggcctt
     9841 gcgctcgcgc aggtaggggt cgtccatctc gtcgaactga cggatcagca cctccagctg
     9901 gctggccagc gcccattcgg cgttgtacag gcgctcggtg atccagcggc ggatctcgga
     9961 cgtcagcgcc tcgtcctgca gcagcatcag gtgcacgtcg agcatggcgc ccagctcggg
    10021 cggcaggtcg cgcggcatgt cggcctgcat gcgctgcagc tcttctacca cggcgttgcg
    10081 ggcggcgcgc aggcgggcga tttcggcctc gacctgttcg ggcgcgatga agtagtgcgt
    10141 cacgtccacc aggctggagg ccaccagcac ggcgcgcccg atggcgatgc cgcgggtgac
    10201 gctgatgccg tggatggaaa agctcattgc ccctcgccga acttgtcgtc gatcagtgcg
    10261 cgcagcgcgt ccatcgcctc ctgctcctgc gcgccgtcgg tctccagcgt gaccacgctg
    10321 ccgatgccgg cggccagcat catcacgccc atgatgctct tggcgttgat gcggcgctcg
    10381 cccttgctca tccagacttc gcaggggtag gcggcggcca gcttggtcag cttggcggcg
    10441 gcgcgggcgt gcaggcccag cttgttgctg atggtgatgt cggtcttgat catgcggcgc
    10501 ccttcagcgc gggttcggtg acgacgggcg gctccaccac caccacgccg cgcatgccgc
    10561 cgtccagcgc gcgctgcgcc atgtcggcca gcggctcggc gcggtaggtg agcgcgcgca
    10621 gcagcatcgg caggttgacg ccggccagca gccgcatgcg ccggccgtcg atcaggcgcc
    10681 gcgccacgtt gctgggggtg gcgcccagca cgtcggtcag caccagcacc gggcgctcgc
    10741 cccagccggc gcacagcgcc tgggcgcgcg ccagggtctg ctcgggcggc tcgtgggcct
    10801 ggacgtcgag cgcaccgatg tcggcggcgg cttcggcaaa cacgtgcagc gcgcattcgc
    10861 gcagggcgtg cgccagcggg gcgtgggcga tcagcagaac ggcgttgctc atggcaggct
    10921 ggattatcgg cgcccggccc gcaggtggtg cagccagcag gcggcgctga gcagcccaaa
    10981 ggcgcccagc gcgccgcgca gcgcgtcggc ctcggaccag cccagggcgg ccagcgcgtc
    11041 ggcgcccagg ccgatgcccc actgcaccag gaagatgccg acgaagatca ccaggttgaa
    11101 ggccgacagc gcgcgcccgg ccacttgcgg cgggtaggcc agcgccagca ccggctgcac
    11161 ctgggccgac ggggtgctca gcacgcagta cagcgccagc agcgccacgc cccagccgcc
    11221 caccgccggt ccggccgcca ccagggccgc cagcgccacg aagctcaacg gctgcacgcg
    11281 cgccacgatg gcgtcgatgc cgatgccgcg cgccgtcagc cgcggcagca gcaggcccca
    11341 ggcccagaag gtcagcagca tgctggcgtt gatccagaac aggccggtgg ccgcttccag
    11401 cgggctgcgc cccaccacgc gggtcagcca cggcccggcc cacagcgact ggatcgccac
    11461 catgccgccg aaactgacca tgccgagcgg ccaggcgcgc cggaattcgg cctgccgcac
    11521 gatctgggcg taagagccat tccagcctcc agcgcccgcc cgatctgcgc catcagctat
    11581 gctattggta gcatccgggg ggcgccagcc gggcacccgc agtgcgatca ccgccatcgc
    11641 cagcagcccc agcagcgcca gcccccagaa cagcggccgc cagcccagca gcggcagcag
    11701 ccactgcacc ggcagggtgg acgccagcat gccgagcgag ccggtcatga gcatccaggc
    11761 gttggcgcgc agctgcgtgg cggcgtcgaa ccagcggcgg tagccggtca gcggcgccat
    11821 cagcccggcg gccacgccca tgcccatcag cacgcgcgcc gccagcaggc cggcaaagcc
    11881 ctgcgccagc gcgaacacgg cacagcccag cacggccacc gccagcagcg ccacgctgac
    11941 gcggcgtggc ccgtagcggt ccagccaggt gcccagcggc agctgcatgg cggcaaagcc
    12001 cagaaagtag ccgcccgcca gcaaaccgag gtcgcgcgcg tgcagcgcga agtcggcggt
    12061 cagcaccggc gacagcgtgg cggtgacggc gcgcagcagc gccgacagaa agtaagcgcc
    12121 tgcaaacgcc agaaacacca gcaccgcggt gcggcgcggc aacagctcgg cacccgcgct
    12181 catggccagc ggatggcgtc gaagaaggcg tcggccgcct cggggcgcag gcgctcggcg
    12241 tagaccacgg cgtgcagcaa ctcggtgccg ccgccggcgg ccgcttgcgc gctccacacc
    12301 gcctgcgcca tcaccggccg gccgtcgggc cgctggccgc gggcggtcag gcgctgggca
    12361 tggggcaggg cgcgggcgcc gggcggcgtg aacgggcgcc gctcgggcgt gccctgggcg
    12421 cgcatgtggg ccagcgtggc ctgctgccag ccggccagca cggcgtcggg gtcgcgcccg
    12481 gccggcagct gggcggtcat cagggcgaag gtggcgccgc cggcgtcgca accggccacg
    12541 ctgagcgcgg tcggcacgcc tcccagcggc acgtcgcggg tggtgcggtc gggtttgcag
    12601 ggcagcaagg caatggccgg cgaaggcgcc aggcgcactt cgcgccagtc cagcgtgggc
    12661 gtgcaggccg ccgcgcccag ggccaccgcc agcacgaggg cggcgcggtg caacaacatg
    12721 cggcgcatta tcggccggcg gctgggcgag aatgcgcgca tgaactcgct cgatggcatc
    12781 cgcgtgctcg acctctcccg cgtgctggcc ggtccctggt gcacccagac gctggccgat
    12841 ctgggcgccg acgtgatcaa gatcgaacgc cccggcagcg gcgacgacac gcgcagctgg
    12901 ggcccgccct tcctgcgcgg cgacgatggc accgaatcgc gcgaggcggc gtactacctg
    12961 ggggccaacc gcaacaagcg ctcggtcacc tgcgacatcg cgcagcccga gggccagcag
    13021 ctgatccgcg agctggtggc gcattgcgac gtgttcatcg agaacttcaa ggtcggcgac
    13081 atggcgcgct acgggctgga ctacgccgcg ctgtcggccc tcaacccgcg cctggtgtac
    13141 tgcagcctga ccggcttcgg ccagaccggg ccgtatgcgc cgcgcgccgg ctacgactac
    13201 atcatccagg gcatgggcgg gctgatgagc gtgaccggcg agcgcgacga ccagggcggc
    13261 ggtccgcaga aggtgggcgt ggcggtggcc gacctgttca ccggcatgta cgccaccgtc
    13321 ggcattctgg cggcgctgcg ccacgccgag cgcaccggcg ccggccagca cctggacatg
    13381 gcgctgctcg acgcccaggt cgccatgctg gccaacctgg gcgccaacca cctggtggcc
    13441 ggcaccgtgc cggggcgcat gggcaatgcg cacgtcaaca tcgtgcccta ccaggtgttc
    13501 gaagtcgcgc cgggcgacgg cggtgacaag gacttcatca tcctggcggt gggcaacgat
    13561 ggccagttcg ccaagttctg cgccgtggcc ggccagcccg agctggcgcg cgacgagcgc
    13621 tttgccacca acgccgcgcg cgtgcgccac cgcgccgtgc tggtgccgct gctcgaagcc
    13681 atcctgaaag cccgtgccaa ggccgactgg ctggccgcgc ttgaagtggc cggcgtgccc
    13741 tgcggcgcca tcaaccggct ggacgaggtg tttgccgacc cgcaggtgcg ctcgcgcggc
    13801 atggtcgacc actggacgca cccgctggcg gccgagctgc ggctggtggc cagcccgctc
    13861 aagctgagcg ccacgccggt gcgcaccgac cggccgccgc cgctgctggg ccagcacacc
    13921 gacgaggtgc tgaccgagct gctgggcgca gacaccaagc ggctggccgc cctgcgggcc
    13981 gcccgggtga tccagtgaac actatcatta caatagctgc tgacgcttga tgggtatgtc
    14041 ctgcgggccg atttgatacc ttatccaggc accggccggt cccccgtcat ccgtcgctct
    14101 tcgaggtacc tccatggcca actacctgct cgttcacggc gcctggggcg gcgcctggta
    14161 ctggcggccg gtgcagcacg cactgatccg cgccggccac aacgcccacg ccgtcaccct
    14221 gaccgggctg ggcgagcgcg cccacctgct gtcgccggcc atcaccctgg gcacgcacat
    14281 cgccgacgtg cgccaggcgc tggcggccga ggagctgagc gactgcatcc tggtggtgca
    14341 ttcctacgcc ggcatgctgg gcaccgccgt ggccgaccgc gagccgcccg gcacgctgcg
    14401 ccatctggtg tatgtcgatg ccgtggtgcc caagccgggc gagagctgga gcggcacaca
    14461 cgccagcggc acccggcgcg agcgcctgca tgcggccgaa atcagtccca cccacagctt
    14521 tccggcgccc gacccggggg tgttcggcct gtcggcggcc gaccacgcct gggtggcgcg
    14581 gcgccaggtg ccgcaccccg gccacaccta cctggagccg ctggagttcg acccgcgccg
    14641 ggtggcgcgc gtgccgcgca ccttcatcga ctgcacccag cccaagctgg ccaccatcga
    14701 cgtcatccgc cagcgcgtgc gcgacccggc gttctgggac ggcgcctggc tgcccggcag
    14761 ccgcatcgtg gaaatggcga ccgggcacga tccgatgatc agcgagaccg aggcgatgac
    14821 acgcctgctg ctggagctgg cctgacagga gcggccgaac tttcgtgcgc ggcagcggtc
    14881 ccacatgacg ccgcacccat gcgcgacgca gggccaagcc cccaaactta aaatcgctgc
    14941 cgtgaacacc tcccgccgca tcgccctcgc cagcctgctg ctgggcgcca gcctgctgag
    15001 cgcctgcggc aacgacgctg ccccgccctc gaccttcgtg ctgctggacg gcagcagcca
    15061 gaccacggcc gacctcaagg gcaaggtcac cctggtcaac ttctgggcca ccagctgcac
    15121 cacctgcgtg gccgaaatgc ccgacatcgt cgccacctac gacaagttca aggcccgcgg
    15181 ctacgagacc atcgccgtgg ccatgcaata cgacccgccc agctacgtgg tcaacttcgc
    15241 cgaaacgcgc aagctgccgt tcaaggtggc gatcgacaac accggcgcgg tggccaaggc
    15301 ctggggcgac gtcaagctga cgcccaccac ctacgtggtc aacaaacagg gcgagatcgt
    15361 caagcgctac gtcggcgcgc ccgacttcag cgagctgcac cagctgatcg aaaaactgct
    15421 ggcggcctga acccgcctcc cccgagccag ccagagccgt gccacccggg gcacggcttt
    15481 ttgtcggcca tggcgcgttc cgaaccgcca gacgggcgcg agcagctgtg ttttgaacct
    15541 atgggttcag ccttggtgtc tggaacaccg ttcgggctga gcctgtcgaa acccttcgac
    15601 aggctcaggg tggacgcatc aacgttcaag tctgcgaagc gaggggtgga acagccaacg
    15661 cgcgcccggt cacgaccgcc gcaccgcatc acgctgggcg ccatgcccac ggcacggtca
    15721 tcgaagtcag aggcgccgac gatagttgtc gtggcagttc ttgcagctct gcccggcggc
    15781 gccgaacacg gccttcaggt tgtccaggtt gccgctcttg gccgcggcgg agaccttggc
    15841 cacctcggcc tgcatcttgt cgcgcaggtc ggtgaacttg gcctgctcct tccagatttc
    15901 gggcttggcg ccggtctggc ccttgtccga gccctcgacg aaggcgtgga acggcagctt
    15961 ggacgcgatg gcgatgacgt cgccgtcgat cgccgcctgg gccgagttga acggcacctt
    16021 gccctgcacc atggcaccca ggctgccgaa atggtagccc agcagggtga acgcgccgcg
    16081 gcggtagtgc acggcgtctt cggccttggc gtactgctgc tgcgcgaagg ttggcgccgc
    16141 ggcgccggcg atggccagcg tgaacgccag gacactgagt ttcttcttca tctgaacgac
    16201 tccttttggg acggggtcgg cgccggcgcg gcgccagcca ccgagggaca aagcgtgccc
    16261 gtggcaacac cgggcgccgg aatcactaca ctgtgacccc gcaacgcacc gcaccgccag
    16321 gtccgccgcg caagcatagc cggttgggcg ccaggccgtg cggcgtgttc gcgcttgacc
    16381 ctgtatcgcc cttcgctcga tcgtccgcca tgcacaccgt ccgcatctgg gatctgccca
    16441 cccgcctgtt tcactgggtt ctgttcgtct gcgtcgtggc gctggtggtc accgccaagg
    16501 tgggcggcaa cgccatgaac ctgcacctgc ggctgggcta cgtggtgttc accctgctgc
    16561 tgtttcggct gctgtggggc ttcgtgggcg gtcactggtc gcgctttgtc agcttcttcc
    16621 ccacgccggg gcggctgacg cgctacctgg gcgggcgcgc cacgaccgcc gacaccgccg
    16681 gccacaaccc gctgggcgcg ctgtcggtgc tggccatgct gctggtgctg ggcgcgcagg
    16741 tgggctccgg cctgttcagc gacgacgaga ttgccttttc cgggccgctc acggccctgg
    16801 tgtccaacgc cacggtgggc cgcctgaccg aatggcacag ccactggggt cagtacctgg
    16861 tgatcgggct ggtgctgctg cacgtgctgg cgatcgtggt gtaccggctg cgacgccaga
    16921 acctggtggg gcccatggtg gtgggcgaca agacgctgcc cgagccggtg accgcggcgc
    16981 gcgacagcgc cggcacgcgc ctgctggcgc tggccctgct gggcgtggcg gcggcgctgg
    17041 tgtacgcggt ggtgcagctc ggcagccgcg gtgccctggg gtagactgga cacgaccgcc
    17101 gccgtgcccg cgcccatgct gcgcaccgaa gttcagtgac cacccgcccg ccgatccacc
    17161 ttgcccccgc ccgggagccc gcgcactacg acgcggcgcg cgccctgttt cgtgagtacg
    17221 ccgatcagct cggggtcgac ctgtgctttc agggcttcga ggccgaactg gctggcctgc
    17281 ctggcgacta ccaggaaccg ggcggcacgc tgctgctggc ctgggtcgac ggcgagctgg
    17341 ccggctgctg cgcgctgcgc ccgatcgagg acgtggacta cgccaacgcc gccgaaatga
    17401 agcggctgtt cgtgcgcaag gccttccgcg gcttcggcct gggccgccag ctggccgagg
    17461 cggtgctgga agccgcggtg cgcctgggct acgacagcgt gctgctcgac acgctggacg
    17521 acatggaggc ggcgcgggcg ctgtacgaag acctgggctt cgtcgaaatc ccgccctact
    17581 accacaaccc gctgccgggg gcgcactacc tgaaggtcga gctgcgcgtc tgacggcgcc
    17641 gccgcgcccc cgtgaatgaa caataaatag gccgttggcg cgcgcccagc ttgcgccagc
    17701 agctactatt tatgtagctt gcgaagccag actcaggctg cgcgcagctg cgccagcagg
    17761 gtggcgccgt cgctcacctc gaacttgcct ggcgcttcga cgttgagtgc cgccaccttg
    17821 ccgtccttga ccagcatcga gtagcggttg ctgcgcagcc ccaggccctt gccgtgcagg
    17881 tccagcgtca ggccggtggc ctgggcgaac gcggcgtcac cgtcggccag catgcggatc
    17941 tggccggcgg tcttctgctc gcgcgcccag gcgcccatga cgaaggcatc gttgaccgac
    18001 aggcaccaga tctcgtccac cccggccgcc ttcagcgccg cggcgtgctg caggtagctg
    18061 ggcacatgct tggccgagca ggtgggcgtg aaggcgcccg gcacggcgaa gacggcgatg
    18121 gtcttgccgg cggcagcctt ctgcacctcg accgggttgg ggccgatgct gcagccgttg
    18181 ccttcgactt cgatgtattc cgacagagtg acggcgggca gcgcgtctcc gaccttgatc
    18241 atgcggggct ccttgaaggt gtgtgggggg tgccgcggcc gtgacgcggc gcggcggtac
    18301 acacgaaaac agcccacgca ttgtgggctg tttcgggtcg gcgcgctggt cgcgccgggc
    18361 tttgggctga gcccgcggcg ggttttcaga cctgagcggc cttctgcacc aggcgggtgg
    18421 cgacccagtt cttggtcttg gacagcgggc ggctttcggt gatctcgatg gtgtcgccca
    18481 gcttgtactg gccgttttcg tcgtgcgcat ggtacttgga cgagcgcatc acgatcttgt
    18541 cgtagatcgg gtgcttgacg cggcgctcga ccagcaccgt caccgtcttg gcgcgcttgt
    18601 cgctgacgac cttgccgatc agggtgcgct tgagggatgg ttgggattcc gtcatggtca
    18661 ctccttactt ggcggcggcc tgcttttcga cgatcagcgt cttggcgcgg gcgatggcgc
    18721 ggcgcgtggc cttgagctgg ctggtgttgc tcagctgctg cgtacccttc tgcatgcgca
    18781 ggttgaaatg ggctttctgc agctccttga cctcggcctg caggccggcg acgtctttct
    18841 gacgcagttc tgcggttttc atctgacgtt cctcctgctc actggccaat catgcgggcg
    18901 acgaaggtcg tgcgcagcgg cagtttggcg gcggccaggg caaacgcctc gcgcgccagc
    18961 tcttccggca cgccgacgat ttcgtacagc accttgccgg gctggatctc ggccacgtag
    19021 tactcggggt tgcccttgcc gttacccata cggacctcgg ccggcttggt ggagatcggc
    19081 ttgtcgggga acacgcggat ccagatgcgg ccaccgcgct tgacgtggcg cgagatggcg
    19141 cggcgcgcgg attcgatctg gcgcgcggtc aggcgaccgc gatcggtgga cttgaggccg
    19201 aagtcgccaa aggccacggt attgccacgc gtggcaatgc cggtgttgcg gcccttgtgc
    19261 tccttgcggt actttctgcg tgcgggttgc agcatggttt actctccttg ggcgccgtcc
    19321 gcggctgcag cgggcgcggc tttgcggacg cgcttaacgg cgggtttggc ctcggccccc
    19381 tggccttcgg cgggtttgtc actgccatcg gcgggcgcac cggagccgcc gccacgcgcg
    19441 tcgcggcgcg ggccaccgcg gcgatcgccg cccgggccac cgcggccgtc acggcgcggg
    19501 ccacgcggac cgcggcgctc ttcgccttcg gggcgcggcg tgctgtccag cgagggggca
    19561 tcgttgcggc ccagggtgtc gcccttgtag acccagacct tgacgccgat ggcgccgtaa
    19621 gtggtgtggg cctcggagaa gccgtagtcg atgtcggcgc gcagcgtgtg cagcggcacg
    19681 cggccttcgc ggtaccactc ggtgcgggcg atctcgatgc cgttcagacg gcccgaggac
    19741 atgatcttga tgccctgggc gcccaggcgc atggcgttct gcatggcgcg cttcatggcg
    19801 cggcggaact ggatgcgctt ttcgagctgc tgggtgatgg aatcggcgat cagctgggca
    19861 tcgacttcgg gcttgcgcac ttcttcgatg ttcaccgcca ccggcacgcc cagctggtcg
    19921 gccagttctt tcttgagttt ctcgatgtcc tcgcccttgc ggccgatcac cacgcccgga
    19981 cgcgccgagt agatggtgat gcgagcgttc ttggccggac gctcgatcag cacgcgcgag
    20041 acggcggcgt tcttgagctt ggctttcagg tactcgcgca ccttgatgtc ttcggccagc
    20101 atgccggcga agtcacggtt gctggcgtac cagcggctgg cccagttgcg gctgaccgaa
    20161 agacggaagc cggtcggatg gattttctgt cccatagtct tctcgtggcc ttcctcagtt
    20221 gcccaccgtc acgtacacat ggcacgtggg cttggacagg cgcgcaccgc ggcccttggc
    20281 gcgggccgcc gagcgcttga gcgtcatgcc ctgctcgacg tagatggtct tgacacgcag
    20341 ttcgtcgatg tcggcaccgt cgttgtgctc ggcgttggcg atcgccgatt ccagcacctt
    20401 cttgacgatg acggcggcct tcttctgcgt gaagttcagg atgttgagcg cctgatcgac
    20461 tttcttgccg cggatcaggt cggccaccag gcggcctttg tccaccgaca ggcgaacgcc
    20521 gcggaggacg gcacgtgttt ccgttgctgc catggtgatt ccttatttct tggctttctt
    20581 gtccgccgcg tgacccttga aggtccgcgt cagcgcaaat tcgcccagct tgtggccgat
    20641 catctggtcg gtcacgtaca ccggtacgtg ctgcttgccg ttgtgcacgg cgatcgtcag
    20701 gccgatgaac tcgggcagga tcatggagcg gcgcgaccag gtcttgatgg gcttcttgtc
    20761 cttgctggcc acggccttgt cgaccttggc gagcaggtgg tggtccacaa acggaccctt
    20821 tttcagagaa cgagtcattt gcggaggtcc ttacttcttg cggcgcgaca cgatcatgtt
    20881 ctgcgtgcgc ttgttgctgc gggtgcggta gcccttggtc aggttgcccc atgggtcaac
    20941 cggatgtcgg ccggatttgc tgcgaccctc gccgccgccc atcgggtggt cgaccgggtt
    21001 catcacgatg ccgcgcacgg tcggacggat gcccatccag cgcttgacac cggccttgcc
    21061 gaactggcgc aggctgtgct cttcgttggc cacttcaccg atggtggcgc ggcactcgat
    21121 gtgcacgcgg cgcacctcgc ccgaacgcat gcgcacctgg gcgtaggtgc cctcgcgcgc
    21181 cagcagcgtg gccgaggtgc cggccgagcg cgcgatctgc gcgccggcgc ccggcttgag
    21241 ctcgatgcag tggatggtcg aacccaccgg gatgttgcgg atcggcagcg tgttgccggc
    21301 gcggatcggc gcttcggagc cgctcatgag ctggctgccc acctccacgc cacgcggtgc
    21361 gatgatgtag cggcgctcac cgtcggcata gcacaccagg gcgatgtggg ccgagcggtt
    21421 ggggtcgtac tcgatgcgct cgaccttggc ggcgatgcca tccttgttgc gacggaaatc
    21481 gatcacgcgg tagtggtggc gatggccgcc gcccttgtgg cgcgtggtga tgtggccgtt
    21541 gttgttgcgg ccggccttct ggaactgggg ctccagcagc ggggcgtaac cctcgccctt
    21601 gtgcaggtgg tcgcgcgaaa tcttgacctg gccacggcgg cccggcgacg tcggtttgat
    21661 cttgatgaca gccatggctt acgcagcctc cccggacagg ttcagctcct ggccttcctg
    21721 gagcagcaca taggccttgc gcacgtggtc acggcgaccg atgctgcggc caaagcgctt
    21781 ggtcttgccc ttgatgttgg ccacggacac gccacggacc ttcacgttga acagcagctc
    21841 gaccgcggcc ttgatctcgg gcttggtggc gctgcgcagc accttgaagg tgacggcgtt
    21901 ggacttttcg gccaccatcg tggccttctc ggaaatcacc ggagcgacga gcacctgcat
    21961 caggcggcct tcgtcgaact ggcgctcttt cggcgtcggg ttgatgcggc tcatgcgaac
    22021 atctccttga gctggtcgat ggcgcccttg gtgaccagca ccttcttgta gtgcaccagc
    22081 gacagcggat cggcgtagcg cggctcgacc accagcacgt tgccgaggtt gcgcgacgcc
    22141 aggtacaggt tctcgtcgac ctcgtcggcg atcaccagca cggagtcgag cttcatgtcc
    22201 ttgaacttct gcgccagctg cttggtcttg ggcgaatcga gcttgatcga ctcgaccacc
    22261 gccaggcggc catcgcgcac cagttgcgac aggatggacg ccatgccggc gcggtacatc
    22321 ttcttgttga gcttctggct gaagttctcg aacgggctgt tcgggaagat gcgaccgccc
    22381 ccacgccaca gcggcgagga ggtcatgccg gcgcgcgcgt tgccggtgcc tttctggcgg
    22441 aacggcttct tggtgctgtg cttgacctgg gcgcggtctt tctgcgcccg cgtgccctgg
    22501 cgggcgttgg cctggtaggc caccaccagc tggtgcacca gcgcttcgtt gtagtcacga
    22561 ccgaacaggg tttcgggcgc gtccaccttg gacgccgcct gaccttggtc gttcaggagt
    22621 tcgagctgca tcagttggcc ccctcgttgg cagcggcctt ggccttgaca gccgggcgga
    22681 cagtgacgaa accgccacgc gcaccgggca cggcgccctt gatgagcagc agctgacggg
    22741 tttcgtcgat gcggatcagg tcgaggttct gggtggtgac ggtctcgtcg cccatgtgac
    22801 cggtcatctt cttgcccggg aagatgcgac ccgggtcctg cgccataccg atggagcccg
    22861 gcacgttgtg cgaacggctg ttgccgtgcg aggcgcgctg cgagctgaag ttgtggcgct
    22921 tgatggtgcc ggcgtagccc ttgccgatgg aagtgccctg cacgtccacc ttctggccgg
    22981 cggcaaacac ctcggccacg ggcagcgcgg tgccggcggc gtacttgccg gccacattgc
    23041 cgtcgacacg gaattcctgg gtgatttcac cggcttcgac gcccgccttg gcgaggtgac
    23101 cggcttccgg cttggtcacg cgagaggcct tgcgcgaacc gaacgtgacc tgcagggcca
    23161 catagccgtc gttctcctgg gtcttgacct gggtgacgcg gttgtttgac acatcgacca
    23221 ccgtgacggg cactgcttcg ccgtcatcgg tgaacagacg catcatgccc accttgcgcc
    23281 ccagcaaccc caatcgattg ctttgactca tttattgctc catgactttc gccgcgcccg
    23341 ctgcaattgg cctgcgcgtt tggtgtgcga aagagggttg aaaaagcctg ccaaaaaatg
    23401 gcaagccgca aatgataacg cgaacttgcc gttctggcaa gtccgcgtcc gaagtctgcc
    23461 cgcgccggcg caacgccggg gccgcgggcg cgccgatcac tgcagcttga tttccacgtc
    23521 cacgccggcc ggcaggtcga gcttcatcag cgcgtccacg gtcttgtcgg tcgggtcgac
    23581 gatgtccatc aggcgctgat gggtgcggat ttccagctgg tcgcggctgc tcttgttgac
    23641 gtgcggcgaa cgcaggatgt cgaagcgctt catgcgcgtg ggcaggggca cggggccctt
    23701 gaccacggca ccggtgcgct tggcggtgtc cacgatctcg gcggccgact ggtcgatcag
    23761 cttgtagtcg aacgccttca ggcggatgcg gatcttctgc ttggttgcca tgacggtccg
    23821 ctccttactc gatgatcttg gccaccacgc cggcgcccac ggtgcggccg ccttcgcgga
    23881 t
//
LOCUS       DAOQTZ010000037        22710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2661_length_22710_cov_13.218539, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000037 DAOQTZ010000000
VERSION     DAOQTZ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 22710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22710
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2661_length_22710_cov_13.218539"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKN51_06255"
     CDS             complement(<1..365)
                     /locus_tag="PKN51_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HNT84737.1"
                     /translation="MQALIKVLCSAACAGALLLAGAAHANYPDRPVKLIVPYAPGGIT
                     DNLARALAEGVSRELKQPIVVENKAGASAMIGTGMVANAPADGYTLLMASNGNMTVTP
                     LVMKKLTYDPIKELRVIAIV"
     gene            complement(399..1181)
                     /locus_tag="PKN51_06260"
     CDS             complement(399..1181)
                     /locus_tag="PKN51_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNT84738.1"
                     /translation="MTDSILHAEAFDGVLQLQLRAPEVRNALSAEMKRALHEQARRFV
                     EDDGLRCLLITGCDAVFCAGGDLRTMQSDRSTVGVRRRMALTHASVRLLAECEKPIVT
                     AVNGAAVGAGLSLALMGDVVVAAEDAYFMAGFANVGVLPDMGVLYHLPRTVGRVQAND
                     LLLSNRRVNAQEALELGLVSRVLPADGFLAQAQALARSIAAGPAVSFGLARALMAGAY
                     KDSFGEFLLKESLAQAVVFGTDDFAEGVSAFGDKRKPRFTGR"
     gene            complement(1178..1975)
                     /locus_tag="PKN51_06265"
     CDS             complement(1178..1975)
                     /locus_tag="PKN51_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT84739.1"
                     /translation="MSKRYDFQGCNVFVSGGTSGINLGIAEAYAAAGARVAVMSRNAE
                     RVADAQARLAGHGQPALGLVGDVRDPARVQQVLAEVHAAWGAIDVLVSGAAGNFLARA
                     LDLSPNGFKTVIDIDLMGTFHVLRAAHPFLRQPGASIINISAPQSYNPAPLQVHACAA
                     KAGVDQVMRVLAMEWGSHGIRVNSIVPGPIEGTTGLAKLASDDARARMAERVPLGRLG
                     RLNDIAAMALFLGSEDASYITGALIPVDGGISLLGARNHAIESGAQA"
     gene            complement(1972..>3483)
                     /locus_tag="PKN51_06270"
     CDS             complement(1972..>3483)
                     /locus_tag="PKN51_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNT84740.1"
                     /translation="IIHWYAQDPAVDVIAAYVEGVTRADVFVNALNAARQARKPVILL
                     KVGRSAVGHHAAQSHTASMAGDDAVTAAVLREFGVVQVRSAEELLDVAYVATRRVYPA
                     ANTLGVATVSGGVGVLISDIAEDEGLAMPPMPEAAQQSLRALLPYASPVNPVDCTAQA
                     VNDSSLVQRFTEAMVEQGGYTSVLSFYAQLADVPVVGHRLLADLSAVRQRHPDRLFVV
                     SATMSDERRRAYDDAGLVVLEDAARATRAIAAMGRLGEAFARPPTQVAASMDAIALSP
                     APLSESQAKQLLTEHGMACSPERLCIDQETAVLAARELGWPVVLKIVSPDIAHKTEIG
                     GVLLNVDSEAAVRAGFDTLCQRAARHAPDARIDGVLVARQLQGGVECLMGVQRDPVFG
                     PVAVFGLGGIFVEALQDVVLHRCPFDEQEAERMIRSIRAAAVLTGIRNRPPADIPALA
                     RMLSRLSVFAHRAGDTLESIDLNPVLALPAGQGAFALDALVQTRNPSHEVSCP"
     assembly_gap    3484..3583
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3584..3872)
                     /locus_tag="PKN51_06275"
     CDS             complement(<3584..3872)
                     /locus_tag="PKN51_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNT84741.1"
                     /translation="MSAAADFPSNLRCLMAPDSVAVIGASSDPSRIGGRPIDWMRRAG
                     FQGAIYPVNPTRDEVQGLKSYASIDDLPATPEAAIVAVPAPQVLATVEALGA"
     gene            complement(3869..4774)
                     /locus_tag="PKN51_06280"
     CDS             complement(3869..4774)
                     /locus_tag="PKN51_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT84742.1"
                     /translation="MLNGKVVVVTGAGNGIGRAIALAMAREGAAVVVNDIGTGLTGEG
                     RDSGPGQQVVDEIRAAGGRAALSTDSVAEASSAERIVQSAIDHFGRIDAVVNNAGILR
                     DRIFHQLSVADWEAVIQVHLNGAFYMSRAAARWFKEQQSGSLIHMVSTSGLIGNLGQA
                     NYSAAKLGMVALSKSIALDMARFNVRSNCISPFAWSRMTSSVPTDTPEKAARVERNKQ
                     MTPDKIAPVAVYLASDAAQAVNAQVFAVRNNEIVLMSQPRPLRSVHRGEGWTPQTVAE
                     HAMPALRASFVGVDRSSDVFCWDPV"
     gene            complement(4815..>5774)
                     /locus_tag="PKN51_06285"
     CDS             complement(4815..>5774)
                     /locus_tag="PKN51_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNT84743.1"
                     /translation="GLAEYLGIHPRLTNNNRVGGSAFQTYVLWAALALAAGQCDVALI
                     AYGSNQRTASGKLVRSLLPSPFEAPYQALVPASAYALAASRHMHEYGTTRAQLAQVAV
                     AARQWAQLNPEAFVREPLSVEQCLAARMLSSPLSVRDSCLVTDGAAALVMTRGDRARD
                     LCLHPVYVLGAAEATHHRMISAAPDLTTTAAVESGARAYAQAGITAADVDVVQLYDAF
                     TINTLLFLEDLGFCPKGEGGRFVEGGRIAPGGALPVNTNGGGLSCVHPGMYGLFALVE
                     AARQLAGEAGERQITGAEIALAHGNGGELSSQATVVLGSTSTV"
     assembly_gap    5775..5874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5875..6167)
                     /locus_tag="PKN51_06290"
     CDS             complement(<5875..6167)
                     /locus_tag="PKN51_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNT84744.1"
                     /translation="MGTPTDILTPERSWLEALAQGRFLLQRSASSGQFVFPPRVAEPR
                     SGATDLQWVEASGAGVVHAVTVISPKPPAAPYTVALIDLAEGVRVMGRVEGLPP"
     gene            complement(6176..7342)
                     /locus_tag="PKN51_06295"
     CDS             complement(6176..7342)
                     /locus_tag="PKN51_06295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT84745.1"
                     /translation="MSFESTPEQQAIREAVLQSCAPFGEDYWLEHDRTGEFPHQFYAA
                     MAQAGWLGMAIPEAYGGSGLGVTEAALMMQTVAETGAGMSGCSAFHINIFGLMPIVVF
                     GTEAQKQRFLPPMVQGQVKSCFGVTEPNTGLNTTQLKTRAVLKGDRYVVHGQKVWIST
                     AQVADKILLLARTTPIEECKSHSHGLSLFYTDLDRNKVAVRLIEKMGRKAVDSNELFI
                     DGLEIPVEDRIGEEGQGFKYILHGMNPERILIGSEAIGIGRAALRKAATYAQERVVFD
                     RPIGKNQGIQHPLAANWMELEAAHLMMLKAAWLYDQGRDCGAEANATKYLGAEAGFHA
                     CERAILTHGGFGYAKEYHVERYLREVMIARIAPVSREQILSYIAERVLGLPKSY"
     gene            7477..8304
                     /locus_tag="PKN51_06300"
     CDS             7477..8304
                     /locus_tag="PKN51_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNT84746.1"
                     /translation="MTTTAIDQDRDRTAPPRAPVRVMQIIAALANHRDGLSLAQLSDV
                     LQLPKTSLFSLLRALEEGGYVMSENGHHRLGQETYSLAALIQKIDGFPGNVRSWLNEL
                     HQACQETVLVGVPSKDWTQLIYVDVIEASSWLRYSANVGARRPLYSTSLGLTMLAFTS
                     TERQRHYIESTRLDAVTHHTITSRSALQQALRRIRAAGCACSSGSIEGATGVSAPVFD
                     RDGSLLGAVGLAGPTARYERNADQFRALVVRCGEQMSRALGFTGAYPNVASHPPGPR"
     gene            complement(8393..9526)
                     /locus_tag="PKN51_06305"
     CDS             complement(8393..9526)
                     /locus_tag="PKN51_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT84747.1"
                     /translation="MSVEQVDCVVIGAGVVGLAVARALALTGREVLVLEAEETFGTQT
                     SARNSEVIHAGIYYPPGSLRAGLCVAGRQLLYDYCAARGVQHRRCGKLIVAANAAQQA
                     ELRAIQARAVANGVDDLQWLARDQALALEPQLQCEAALLSPSTGIIDSHGLMLALLGD
                     LEHAGGTLATHSRVNRLVALDGQAPAAIKIEVSSDQETTVLHARSVVNCAGLGAIALT
                     HATQGLAPAHRPPLPQRWAKGSYFTLSERAPFARLIYPVVERDSPSLGVHLTLDLGGQ
                     AKFGPDVQWVASPDDLQVDPARADAFYAEVRRYWPALPDGALQPGYVGYRPKLSGPGE
                     PAVDFRIEGPGQHGVPGLVNLLGIESPGLTSCLAIARHVVGVL"
     gene            complement(9523..10566)
                     /locus_tag="PKN51_06310"
     CDS             complement(9523..10566)
                     /locus_tag="PKN51_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNT84748.1"
                     /translation="MLIFTTAEQADHDPAHEIFRGQRVPCFENPSRAAFVEQALRAAG
                     HTLRRPDTDSADLLSQVHSPRYLQFLRTAWDQWLALDAANAGVQPFPSVWPVRTLRSD
                     VEPANFIARLGLYSMDNGSPMVAGTWVAAKAAADAAASAARALGQGERAVFCATRPPG
                     HHAGPDFMGGYCFINHAAVCAQALRAGGAQRVAVLDVDYHHGNGTQAIFYERADVLFV
                     SIHGDPRTEYPFYLGHADETGAGAGQGCNLNLPLAAGASVATWFDALDTACARVAAHR
                     PDALVVSLGLDTFAGDPISRFQLQTGDFRRLGRRLQQLGLPTAFILEGGYAAAELGQN
                     AAQVLQGFEEGGR"
     gene            complement(10769..12967)
                     /locus_tag="PKN51_06315"
     CDS             complement(10769..12967)
                     /locus_tag="PKN51_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84749.1"
                     /translation="MARNPDNAGGSGKVELSVHVQLQEVDDRQAAPQPAVYAYSAGGK
                     LLAMQPADERGQARLALAVGAEDTAVRVLVGPTLERPEASELLRRGAVARPLRLSLKD
                     PRPKLVLPIDPDSWRCWLLGRCVVRGTLLKRVVSGGLPLELPVCHASVDVWEVDPWPV
                     LVPRLPPWVIDGLRRVIVEPRPPWPEPPGPWPIDGPGPLPPPGPGPDPVPLAQRRAMR
                     AGRTPGIGSGAAVLASFHAAPQAGELKTLAQIGNAQQFQQALIRHPELVRPLLCWFYP
                     HIVTKQKVATAVTDECGQFRAVFWRGCQSSDVPDLYFTAQQRLFGWLNVSIYEPTPVP
                     CHTHWNYVCGTPVTLITTSPWAIACSPCRPYHPGDNWVLFTAVGNTSLKAIHGGGAPG
                     ATGDNLGLLQSGAPWGGVLRPRLDFDNALRESLGVRYYQLSWRKGMAGDFKPLSVEVN
                     RHYAHMVGTDLVIDPYRLGPNHMVVGDQTLELYEIPPALPPFGQWTIANAVLDTENGE
                     FDSRVFSRGLAFEEDGDVVPGSVDESGLYQIKLDLYDAAGNQIDIAAKGIDYYVPDSV
                     SLSGSIPTVKASSVTQPGGGSLVQGNSLVLTLHVDNNHCWAGVGAPWTPSGAADPCCG
                     VLRFAPGASVSMPYVAYHPHGFATHAFSVVRSATQILSPSGGVGSFVITRTVHDMMTL
                     NLPAACAGKPECTTAAFGERLDVYEMATDGWGSDLGYEASDLRAFALSTH"
     gene            complement(13046..14359)
                     /locus_tag="PKN51_06320"
     CDS             complement(13046..14359)
                     /locus_tag="PKN51_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84750.1"
                     /translation="MPDTKESSLARIECCPVLERQPVCDRLDFRYRLPYPVRTGDRQT
                     VRVDLIVHFRLERCSGPLVLGDPVYSTTLLPGEQVRLFSSDRHTRWSYDAESKLAYRH
                     ETTSEESFFTYGMARAVSDLTITEGGSASSSYDESWAEGGGGASVNLFGIIEIGGGGG
                     GGSYDAHSASSFARFLSQHAQSASSSVAAGVRAKSSTAVGEVSTREHREGESSDHFES
                     ASRMFRNPNRCHAVTYLFYRVSKVQKLRFRLVAIQRVVSDPAAPVLSDQRPSVDVAGR
                     ISVLPEVVSASSPQRLAIEERARASALSRLNMTGGNVSLLARPLVGAAAPLGVDVRQA
                     ALAAVDKELAAAGVIDPKTGQVHDKLVAELSWEREEVLPTPGILVKGCLDDCATCEPA
                     LQREIELELERKRLDNEKLKREIELLDQAQQYRCCPVGEAEDDDD"
     gene            complement(14529..15353)
                     /locus_tag="PKN51_06325"
     CDS             complement(14529..15353)
                     /locus_tag="PKN51_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT84751.1"
                     /translation="MADSRIDPMPAPAPRAVWIDLRCMPQPHGLYQDARPHGVMARIT
                     QLADIVGAIARWRPHFVCIDYDYPDGARLDAVPRVRREFPQLPVLMFTDYHTEALAVW
                     ALRHRVWDYRVKPVTVSTLRRLFEVLRCGGAEARAPGGWPAPALPPELREPAGHLRKS
                     LQASARTAAAVAWIGEHYGEPCPIKLMAELCHLSPSEFSRAFHREQGVPFCHFLQHHR
                     VTMAREFLREPGATVSQVAYAVGFNDLSYFGRVFRRLVGVPATQYQRLKAPPQGKA"
     gene            complement(15485..16339)
                     /gene="thiD"
                     /locus_tag="PKN51_06330"
     CDS             complement(15485..16339)
                     /gene="thiD"
                     /locus_tag="PKN51_06330"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNT84752.1"
                     /translation="MTQQYVRVLTIAGSDSGGGAGIQADLKTFAALGCYGMSAITALT
                     AQNTQGVTAIHGVPPDMLAAQIDAVLNDIGAQAVKIGMLHAPDIVHTVAAAIERHHMT
                     QVVLDPVMVSATGARLIEAEAVQALVTALFPRASLITPNLDEAALLLGRPVQAVADMA
                     PAAQALLALGARAVLLKGGHLKGQQVHDLLLVRGAEPLHMCSARLATRNLHGAGCTLS
                     SAIAAHLALGLGLSDAVQAARHYVQQALAAGATVQTGEGVGPLNHGFAPRTMVTLGRD
                     QITSKDVT"
     gene            complement(16391..18070)
                     /locus_tag="PKN51_06335"
     CDS             complement(16391..18070)
                     /locus_tag="PKN51_06335"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNT84753.1"
                     /translation="MTPMGQAPAATKSVAQPIVWSVAGNDSGGGAGLAADQRAAAAFG
                     VHLCPVVAAITAQHSQAVTRVEPVAADLLDAQLAALAADLPPAAIKTGLLGSADNVAV
                     LARWVDRLRQRAPLALVVDPVLGASSGAAFADEAVLRAYRELLLPRATLVTPNEHEAR
                     RLLGGAPPNPRPSGQRAGVVGQPGEPSDVPALAVALRAQGAQAVAITGGDRVHAPGWA
                     LDWIDTEHARGWLALPRLESRHTHGTGCTFASSAAAALALGFVAADALVLAKMATAHA
                     IAHGHAAGQGAGPVAARPGFAVEPARMPLLSCSELDSCWRFLGARQSPIPLETQAPGL
                     YAIVDSAERVRQVVAAGARTVQLRIKTPAAPPAAWQDELRCAIRQALGACREAGATLV
                     VNDHWRLAAELTAGAARGIAVHLGQEDLLALGDAGRAELQACGLPLGISSHSLWELAR
                     ARSLAPWYVACGPVWPTLTKAMPWRAQGLDNLSWWVHMAGVPVVAIGGILEPAQATQA
                     TRSGAAGVCVVRGLGADPAATLPVWQAAIAAGRAAPRLPVPALPHPTLPNP"
     gene            complement(18067..18882)
                     /locus_tag="PKN51_06340"
     CDS             complement(18067..18882)
                     /locus_tag="PKN51_06340"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNT84754.1"
                     /translation="MHDSDHDTWAVGDVRLDSRFLLGTAKYPSPRVLADAIAASGTQI
                     VTVGLKRQLAAGGDNSFVEIIRAAMRTQGARLLPNTAGCTTAREAIQLAHMARELYGT
                     AWVKLEVIGDDYTLQPDPQELVEAARQLTRDGFTVFPYCTEDLVTARRLLDAGCPLLM
                     PWGAPIGSGQGLVNRFALRLLRERLPDVVLIIDAGLGAPSHAAQALEMGFDAVLLNSA
                     VSQSPDPVRMAGAFRLAIEAGRAAYRAGVMARQDFAVATTPVTGNPFLIGNAP"
     gene            complement(18916..20349)
                     /gene="arcD"
                     /locus_tag="PKN51_06345"
     CDS             complement(18916..20349)
                     /gene="arcD"
                     /locus_tag="PKN51_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HNT84755.1"
                     /translation="MNPPKTTKLRVGALTALVVGSMVGGGIFSIPQNTAASAGVGATA
                     IAWLITGVGMLALAFVFLNLALREPELDSGIYAYARASFGPFTGFSSAWGYWFMAVLG
                     NVGYYVLLFSTLGHYWPVFGDGNTPLAVVCASVMLWGTHALVLRGIREAALINQVTTV
                     AKLVPLLLFVLLVALAFQPQVFRADVWGRANPQLGSVLEQVRGMMLVTVWVFIGVEGA
                     SVYSAHAQRRIDVGRATLAGFLFVLALLVAVSLLSMGVMAQPELAGLKNPSMAYVLER
                     AVGHWGGVLISVGLLISLSGALLSWLMLSAETLYSAARDGAAPDWLAGQNARGVPSHA
                     LWLTSACVQAFLLLTLWSNSTYLSLVNLGTAMILLPYTWAAGHALKIALSGARYGVAE
                     GGRRRRDALLSLLALAYALWLVYAGGIKYLLLGALLYAPGALLYAWARRSHGRAVFSR
                     REWLGFLAMLAAAGVAALGLQRGWLSL"
     gene            complement(20365..20595)
                     /gene="thiS"
                     /locus_tag="PKN51_06350"
     CDS             complement(20365..20595)
                     /gene="thiS"
                     /locus_tag="PKN51_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNT84756.1"
                     /translation="MPSHSSDAATLHILLDGHPHAIPAGSTLADVVASLGHAPEAVST
                     AVNGAFVARPARATQRLAEGDQVLLFQPIVGG"
     gene            complement(20750..21916)
                     /locus_tag="PKN51_06355"
     CDS             complement(20750..21916)
                     /locus_tag="PKN51_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT84757.1"
                     /translation="MSPPALSIGIAGAGLLGRLLAWQLSRRGHQVSVFDSAAGPQPVA
                     RSQAGDPYVPTAAGFTAAGMLSPLSELDNAEPAVAHMGFRSLALWPGILAALPGAPEL
                     VVRGSLLLAHRADLGTARRMLDRLHVATATPEWRHLQLPAGEQPLSPAELRTLEPAIQ
                     GPAHAWLLPGEGHIDTVATMIALHAGASGVDWHWGVPVRALQGGDAGGTLLLADGRAL
                     AFDAVVDVRGAGARPELPVRGVRGEVVWLDCPGHGLTRPARLLHPRHFVYMVPRSSRD
                     VVVGASEIESEDRSPVSLRTAVELMAAAHSVLPGLAEARILKLDVNLRPALPDNNPLI
                     EHAGRLLRINGLFRHGWLLAPALVERALAEAPWLDTALASDGRPSRHGAAKAQA"
     regulatory      complement(22081..22189)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22220..22582
                     /locus_tag="PKN51_06360"
     CDS             22220..22582
                     /locus_tag="PKN51_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84758.1"
                     /translation="MTHVRTLLTLALPGALLLGACAGGGHPRPGTSTQQEYEAAMRDQ
                     SPVRVIVREGPISNSDVGRTPEELARQEAGQQCGRASLDAKVLDVQRQRAPGRDATGG
                     VVQFERVRLTFRCERGRP"
BASE COUNT         3502 a         7957 c         7799 g         3252 t
ORIGIN      
        1 acgatggcga tcacgcgcag ttccttgatc gggtcatagg tgagtttttt catcaccagc
       61 ggcgtcaccg tcatgttgcc gttggacgcc atcagcagcg tgtacccgtc agccggcgcg
      121 ttggccacca tgccggtgcc gatcatggcg ctggcgcccg ctttgttttc aacgacgatg
      181 ggttgcttga gctcgcggct cacaccttcg gccagggcac gcgccaggtt gtcggtgatg
      241 ccaccgggcg catagggcac gatcagcttg acggggcggt cggggtagtt ggcatgtgcg
      301 gcgccggcca gcaacagcgc gccggcacag gcggccgagc acaggacttt gatcaaggct
      361 tgcatggtgt ctcctggttg aggtgatgaa ggccgcactc agcggccggt gaaacggggt
      421 ttgcgcttgt cgccaaacgc gctgacgccc tcggcaaagt cgtcggtgcc aaacaccacg
      481 gcctgcgcca ggctttcctt gagcaagaac tcgccaaagc tgtccttgta ggcgccggcc
      541 atcagggcgc gcgccagtcc aaacgagacg gccgggccag cggcgatgga acgggccagg
      601 gcctgcgcct gcgccagaaa tccatccgct ggcagcacgc ggctgaccag gccaagctcc
      661 agcgcctcct gggcgttgac ccggcgatta ctgagcagca ggtcattggc ctgcacgcgg
      721 cccacggtac gcggcaggtg gtagagcacg cccatgtccg gcagcacccc cacgttggca
      781 aagccggcca tgaaatacgc gtcttcggcc gccaccacca cgtcccccat cagggccagg
      841 gacaggccgg cgcccaccgc ggcgccattg acggcggtga cgatgggttt ctcgcattcg
      901 gccagcagac gcacgctggc gtgcgtgagc gccatgcgtc ggcgcacgcc gacggtgctg
      961 cgatcgcttt gcatggtgcg cagatcgccg ccggcgcaga acaccgcgtc gcaccctgtg
     1021 atcagcaggc atcgcaggcc gtcgtcttcg acgaagcggc gcgcctgctc atgcagcgcc
     1081 cgcttcatct cggcgctcag ggcgttgcgc acttcgggcg cgcgcagctg caactgcagg
     1141 acaccatcga aggcctcagc gtgcaagatg ctgtcggtca tgcctgtgct ccggactcga
     1201 tggcgtgatt acgcgcgccc agcaaggaaa tgccgccatc gaccgggatc aacgcgccgg
     1261 tgatatagct cgcgtcctcg gagcccagaa acagcgccat ggccgcgatg tcgttcagcc
     1321 ggcccaggcg acccagcggc acacgctccg ccatgcgggc gcgggcgtcg tccgaggcca
     1381 gcttggccag ccccgtcgtg ccctcgatcg gccccggcac gatggagttg acacggatac
     1441 cgtggctgcc ccactccatc gccagcacgc gcatgacctg atccaccccg gccttggcgg
     1501 cgcaggcatg gacctgcagg ggcgccgggt tgtaggactg cggcgccgag atgttgatga
     1561 tcgacgcgcc cggttgacgc aggaacgggt gagccgcgcg cagcacgtga aaggtgccca
     1621 tcaggtcgat gtcgatcacg gtcttgaagc cgttgggcga cagatcgagc gcgcgggcca
     1681 gaaaattgcc ggccgcaccc gacaccagca catcgatggc gccccaggcg gcatgcactt
     1741 cggccaatac ctgctgcacc cgtgccggat cccgcacatc gccaaccagc cccagggcgg
     1801 gctggccgtg gcctgccagc ctggcctggg cgtcggccac gcgttcggcg ttgcggctca
     1861 tcaccgcaac gcgtgcgccg gcggcggcat aggcctcggc aatgcccagg ttgatgccgc
     1921 tggtgccgcc ggagacgaac acgttgcagc cctgaaagtc atagcgtttg ctcatgggca
     1981 gctcacttcg tgtgacggat tgcgggtctg taccagggcg tccagcgcga aggcgccctg
     2041 ccccgcgggc aatgccagca ccgggttgag atcaatgctc tccagcgtgt cgccggcgcg
     2101 gtgggcgaac accgacaggc gcgacagcat gcgggccagc gccggtatgt cggctggggg
     2161 acggttgcgt atgcctgtca gaacagcagc ggcgcggatg gagcggatca tgcgctcggc
     2221 ctcctgctca tcgaacgggc agcgatgcaa caccacgtcc tgcagcgcct cgacaaagat
     2281 gccgcccaga ccgaacaccg ccaccgggcc aaacaccgga tcgcgctgca cgcccatcag
     2341 gcattcgacg ccgccctgca gctggcgcgc caccaggaca ccgtcgatgc gggcatcggg
     2401 ggcgtgccgg gcggcacgct ggcacagggt gtcgaagccg gcgcgcacgg cggcctcgct
     2461 gtccacgttc agcagtaccc cgccgatctc ggtcttgtgg gcgatgtcgg gagaaacgat
     2521 cttgagcacc accggccagc ccaactcgcg cgcagccagc accgcagttt cctgatcgat
     2581 gcacagccgc tcgggcgaac aggccatacc atgctccgtc agcaactgct tggcctgcga
     2641 ttcggacagc ggcgcgggcg acagcgcgat ggcgtccatc gacgcagcga cctgagtggg
     2701 agggcgtgcg aacgcctcgc ccaggcgccc catggcggcg atcgcgcggg tggcgcgagc
     2761 ggcgtcctcc agcaccacca ggccggcgtc gtcgtaggcg cgccggcgct cgtcgctcat
     2821 ggtggccgac accacgaaca gacggtcggg gtggcgctgg cgtaccgcgc tcaggtccgc
     2881 cagcaggcga tggccaacca cgggcacgtc ggccaactgg gcgtaaaagc tcagcaccga
     2941 ggtgtagccg ccctgctcca ccatggcttc ggtgaagcgc tgtaccaggc tggagtcgtt
     3001 gacggcctgc gccgtgcagt ccaccgggtt gaccggcgag gcatagggca gcagggcgcg
     3061 caggctctgc tgggcggctt cgggcatggg gggcatggcc agcccctcgt cttcggcgat
     3121 gtcgctgatg agcacgccga cgccgccgct gaccgtggcc acccccaggg tgttggcggc
     3181 cggatagacc cggcgcgtgg cgacataggc cacatccagc aactcctcgg ctgagcgcac
     3241 ctgcaccacg ccaaactcgc gcagcaccgc cgccgtgacg gcgtcgtcgc cggccatcga
     3301 ggcggtgtgt gactgggccg cgtggtggcc gacggcgctg cgcccgacct tgagcaggat
     3361 cacgggcttg cgcgcctgcc gggcggcatt cagcgcattc acaaacacat cggcacgcgt
     3421 caccccctcg acataggcgg cgatcacatc caccgccgga tcctgggcgt accagtgaat
     3481 gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgcgcc cagcgcctcc
     3601 accgtcgcca gcacctgggg cgccggcacc gccacgatgg ccgcctccgg tgtggcggga
     3661 aggtcatcga tcgaggcgta ggacttcagt ccctgaactt cgtcgcgcgt ggggttgacc
     3721 gggtagatgg cgccctgaaa tccggcgcgc cgcatccagt cgatgggccg cccgccaatg
     3781 cggcttgggt cggacgaggc gccgatcacc gccacgctgt ctggcgccat caggcagcgc
     3841 aggttgctcg ggaaatcggc cgcggcgctc acaccggatc ccagcagaag acgtcgctgg
     3901 agcgatcgac gccgacaaag ctggcgcgca gcgccggcat ggcgtgctcg gccacggttt
     3961 gcggcgtcca gccctcgccc cggtgcaccg agcgcagcgg acgcggctgg ctcatcagca
     4021 cgatctcgtt gttgcgcacg gcaaagacct gggcgttgac ggcttgcgcc gcgtcgctgg
     4081 ccagatacac cgccaccggg gcgattttgt ctggcgtcat ctgcttgttg cgctccacgc
     4141 gcgcggcttt ttcaggcgtg tcggtcggaa ccgagctggt catgcggctc caggcaaacg
     4201 gcgagatgca gttggagcgc acgttgaagc gcgccatgtc cagcgcgatc gacttggaca
     4261 gcgccaccat gcccagcttg gcggccgaat agttggcctg gcccagattg ccgatcagcc
     4321 cggaggtgga gaccatgtgg atcaggctgc ccgactgctg ctccttgaac cagcgcgcgg
     4381 cggcgcggct catgtagaag gcgccgttca ggtgcacctg gatcacggcc tcccagtcgg
     4441 ccacgctgag ctgatggaag atgcggtcac gcaggatgcc ggcgttgttg accaccgcgt
     4501 cgatgcgccc gaagtggtcg atggcacttt gcacgatgcg ctcggcgctg ctggcttcgg
     4561 ccacgctgtc ggtgctgagc gcagcgcgac cgccggcggc gcggatttcg tccaccacct
     4621 gctggccggg cccgctgtca cggccttcgc cggtgaggcc ggtgccgatg tcgttgacca
     4681 ccaccgctgc gccttcgcgc gccatggcca gagcgatggc gcgcccgata ccgttgccgg
     4741 cgccggtgac gaccacgacc ttgccgttga gcatgttgtt cgttgtcaag aagggactcc
     4801 tggatcgaga gggttcaaac cgttgaggtg ctgcccagta ccaccgtggc ctggctggac
     4861 agttcgccac cgttgccgtg agccagcgcg atctcggcgc ccgtgatctg gcgctcgccg
     4921 gcctcgccgg ccagctggcg cgccgcctcg accagggcaa acaggccgta catgccggga
     4981 tgcacgcagg acaggccgcc gccgttggtg ttgaccggta gtgcgccgcc gggcgcgatg
     5041 cgcccgccct cgacaaagcg accgccctcg cccttggggc aaaaacccag gtcttccagg
     5101 aacaggaggg tgttgatggt gaaggcgtcg tagagttgaa ccacgtccac gtcggcggcg
     5161 gtgatgcccg cctgggcata ggcgcgcgcc ccggattcga ccgccgcggt ggtcgtgaga
     5221 tcgggcgccg ccgagatcat gcggtggtgc gtggcctcgg ccgcgcccag cacgtagacg
     5281 ggatgcaggc acagatcgcg ggcgcggtcg ccgcgcgtca tcaccagggc ggcggcgccg
     5341 tcggtcacca ggcagctgtc gcgcacgctc aggggcgacg acaacatgcg agccgccagg
     5401 cactgctcga cgctcagggg ttcgcggaca aacgcctcgg ggttgagctg cgcccactgg
     5461 cgtgccgcca ccgccacctg ggcgagctgc gcgcgcgtgg tgccgtattc gtgcatgtgg
     5521 cgcgaagccg ccagcgcata ggcggaggcc ggcaccaggg cctggtacgg cgcctcgaag
     5581 ggcgacggca gcagcgagcg caccagcttg ccactggcgg tgcgctggtt gctgccgtag
     5641 gcgatcaggg ccacgtcgca ctggccggcg gccagcgcca gcgcggccca cagcacgtag
     5701 gtctgaaaag ccgagccgcc cacgcggttg ttgttggtca ggcgcgggtg tatgcccagg
     5761 tattcggcca ggccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcggc
     5881 aggccttcga cccgccccat gacgcgcacg ccctcggcca gatcgatcag cgccacggta
     5941 tacggcgctg ccggtggttt gggtgaaatc acggtgacgg catggaccac gccggcgcca
     6001 ctggcttcga cccactgcag atcggtggcg ccgctgcgcg gctcggccac gcgtggcgga
     6061 aacacgaact ggccgctgct ggcgctgcgt tgcagcagaa agcggccctg ggccagcgct
     6121 tccagccagc tgcgctcggg tgtgaggatg tcggtagggg tgcccatgtc tgcgctcagt
     6181 aggacttggg caggcccagc acgcgctcgg cgatgtacga caggatctgc tcgcgactga
     6241 ccggcgcgat gcgcgcgatc atcacttcgc gcaggtagcg ctcgacgtgg tattccttgg
     6301 catagccgaa gccgccgtgc gtcaggatgg cgcgctcgca ggcatgaaaa ccggcctcgg
     6361 cacccaggta cttggtggcg ttggcctcgg cgccgcagtc gcgcccctgg tcgtacagcc
     6421 aggcggcctt gagcatcatc aggtgcgcgg cttccagctc catccagttg gcggccagcg
     6481 gatgctggat gccctggttc ttgccgatcg ggcggtcgaa cacgacgcgt tcctgggcgt
     6541 aggtggcggc cttgcgcagc gcggcgcggc caatgccgat ggcctcggaa ccgatcagga
     6601 tccgctcggg gttcatgccg tgcaggatgt atttgaagcc ctggccttct tcgccgatgc
     6661 ggtcttccac aggaatttca aggccgtcga tgaacagctc gttggagtcg accgccttgc
     6721 gccccatctt ctcgatcagg cgcaccgcca ccttgttgcg atcgaggtcg gtgtagaaca
     6781 gcgacaggcc gtggctgtgg ctcttgcatt cctcgatcgg ggtggtgcgc gccagcagca
     6841 ggatcttgtc ggccacctgg gcggtggaga tccacacctt ctgcccgtga accacatagc
     6901 ggtcgccctt cagcaccgcg cgggtcttga gctgggtggt gttgaggccg gtgttgggct
     6961 cggtcacgcc aaagcaggat ttgacctgtc cctgcaccat ggggggcaga aagcgctgct
     7021 tctgcgcttc agtgccgaac accacgatcg gcatcaggcc aaagatgttg atgtgaaagg
     7081 ccgaacagcc gctcatgccg gcgccggtct cggccacggt ctgcatcatc agcgccgctt
     7141 cggtgacgcc caggcccgag ccgccatagg cctcgggaat ggccatgccc agccagcccg
     7201 cctgggccat ggcggcgtag aactggtgcg gaaactcgcc ggtgcggtcg tgctcgagcc
     7261 agtagtcctc accaaagggg gcacaggact gcagcacggc ttcgcgaatg gcttgttgct
     7321 cgggggtgga ttcgaaggac atggcttggg gtttgttctg atatcggaat atgtgttctg
     7381 ataagagaat tatcagaatc gagctccctc gctgtcaacg cctcttcggg ttattcccga
     7441 gctttgaacg catcaccccg atcccacact gccgccatga ccaccactgc catcgatcag
     7501 gatcgcgacc gcaccgcacc accccgcgcc cccgtgcggg tcatgcagat catcgccgcc
     7561 ctggccaacc accgcgacgg cctgtcgctc gcgcagctca gcgacgtgct gcagctgccc
     7621 aagaccagcc tgttcagcct gctgcgcgcg ttggaagaag gcggctacgt gatgtccgaa
     7681 aacggccacc atcgcctggg gcaggaaacg tacagcctgg ctgcgctgat ccagaagatc
     7741 gacggctttc ccgggaatgt gcgttcctgg ctgaacgaac tgcaccaggc ctgccaggag
     7801 acggtgctgg tgggcgtgcc ttccaaggat tggacgcagc tgatctatgt cgatgtgatc
     7861 gaggccagca gttggctgcg gtactcggcc aacgtgggtg cgcgtcgccc cctgtactcc
     7921 acctcgctgg gcctgaccat gctggcattc acctcgaccg aacgccagcg ccactacatc
     7981 gaatcgaccc gcctggacgc cgtgacgcac cacaccatca ccagtcgcag cgctctgcag
     8041 caggcgctgc gccgcattcg cgccgcaggc tgcgcgtgca gcagtggctc catcgaggga
     8101 gccaccggcg tgtctgctcc ggtgttcgac cgcgacggct cattgctggg tgccgtcggc
     8161 ctggctgggc ccacagcccg ctatgagcgc aacgccgatc agtttcgggc actggtcgtg
     8221 cgttgcggcg agcagatgtc gcgcgcgctc gggttcaccg gcgcctatcc gaacgtcgca
     8281 agccacccgc cggggccgcg ttgaccgtcc gctgaaggcc ccctcgcctt gcagacatcg
     8341 accccgcgcc cggcgacggc gagcagccgt cgcggcgcga ctgcgcggtg gctcagagca
     8401 cgcccaccac gtgccgggcg atcgccagac agctggtcag ccccggcgac tcgatgccca
     8461 gcagattcac cagtcccggc acgccatgct ggccggggcc ctcgatgcgg aaatccaccg
     8521 ccggctcgcc cgggccggag agcttgggcc ggtagccgac gtaacccggt tgcagcgccc
     8581 cgtcgggcag cgcgggccag tagcgccgca cctcggcgta gaaggcgtcg gcgcgcgccg
     8641 gatcgacctg cagatcgtcg gggctcgcca cccactgcac gtccggcccg aacttggcct
     8701 gaccgcccag gtccagcgtc aggtgcacgc ccaggctggg cgagtcgcgt tcgaccaccg
     8761 gatagatcag gcgcgcaaag ggcgcccgct cgctcagcgt gaagtagctg cccttggccc
     8821 agcgctgcgg cagcggcggc cggtgcgcgg gcgccagccc ctgcgtggcg tgggtcagcg
     8881 caatggcgcc caggccggcg cagttgacca cgctgcgggc gtgcaggacg gtggtttcct
     8941 ggtcgctgga tacttcaatt ttgatagctg ctggcgcttg tccgtcaagc gctacaaggc
     9001 gatttaccct tgaatgcgtg gccagcgtgc caccggcgtg ctccagatcg cccagcagcg
     9061 ccagcatcag gccgtggctg tcgatgatgc cggtcgaggg cgacaacagc gccgcctcgc
     9121 attgcagctg cggctccagc gccagcgcct gatcgcgcgc aagccattgc agatcgtcca
     9181 caccgttggc cacggcgcgc gcctggatgg cgcgcagctc ggcctgctgc gcggcgttgg
     9241 ccgccacgat cagcttgccg cagcgccggt gctgcacgcc gcgcgcggcg caatagtcgt
     9301 acagcagctg ccgccccgcc acgcacagac cggcgcgcag cgagccgggc gggtagtaga
     9361 tgccggcgtg gatgacctcg ctgttgcggg cgctggtctg ggtgccaaag gtttcttcgg
     9421 cctccagcac cagcacctcg cgcccggtca gcgccagcgc gcgcgccacc gccagcccga
     9481 ccacgccggc gccgatcacc acgcaatcga cctgctccac gctcatcgtc cgccctcctc
     9541 gaagccctgc agcacctgcg ccgcgttctg acccagctcg gccgccgcgt agccgccctc
     9601 caggatgaag gcggtgggca ggcccagctg ctgcaggcgc cggcccagcc gccggaagtc
     9661 gccggtctgc aactgaaagc ggctgatcgg gtcgcccgcg aaggtgtcca gccccagcga
     9721 caccaccagc gcgtccggcc gatgggcggc cacgcgcgcg caggcggtgt ccagcgcgtc
     9781 gaaccacgtg gccacgctgg cccccgccgc cagcggcagg ttgaggttgc agccctgccc
     9841 cgcgccggcg ccggtctcgt cggcatggcc cagataaaac gggtattcgg tgcgcggatc
     9901 gccgtggatg ctgacgaaca gcacgtcggc gcgctcgtaa aaaatggctt gcgtgccgtt
     9961 gccgtggtgg tagtccacgt ccagcaccgc cacgcgctgc gcgccgcccg cacgcagcgc
    10021 ctgcgcgcac acggcggcgt ggttgatgaa gcagtagcca cccatgaagt ccggccccgc
    10081 gtggtggccg ggcgggcggg tggcgcaaaa caccgcgcgc tcgccctgcc ccagcgcccg
    10141 cgcggcgctg gctgccgcgt cggcagcggc cttggccgcc acccaggtgc cggccaccat
    10201 ggggctgccg ttgtccatcg aatacaggcc caggcgggcg atgaagttgg cgggctccac
    10261 gtcgctgcgc agcgtgcgca ccggccacac ggacggaaac ggctgcacgc cggcgttggc
    10321 cgcgtccagc gccagccact ggtcccaggc cgtgcgcagg aactgcaggt agcgcggtga
    10381 gtgcacctgc gacagcagat cggcgctgtc ggtgtccgga cggcgcaacg tgtggccggc
    10441 ggcgcgcagg gcctgctcga caaaggctgc gcggctggga ttttcaaagc agggcacgcg
    10501 ctggccacga aaaatctcgt gcgccgggtc gtggtcagcc tgttcggcgg tggtgaagat
    10561 cagcatcggt ggcggccggg ctgcgtgtgc gcgcggcgag cgcccatgtt agggcatccc
    10621 aggctgtcgc cagcacctcg gcggggccga caccggggcg ggcgacggca cgcgcccacc
    10681 ccggccgcgc gggcctgccc tcagcctgag ggcagccgtt ggccgcccgt catgttcggg
    10741 cgcacgcgtc ccacggccac cttcgggctc agtgggttga cagtgcgaat gcccggaggt
    10801 cggacgcttc gtagccgagg tcgctgcccc agccgtcggt ggccatttcg tagacgtcaa
    10861 gccgctcacc gaacgccgcc gtcgtgcact ccggcttgcc cgcgcaggcg gccggcaggt
    10921 tcagcgtcat catgtcgtgc accgtgcggg tgatgacgaa gctgccgacg ccgcccgacg
    10981 gcgacaggat ctgcgttgcg ctgcgcacca ctgaaaaagc atgggtggcg aagccatgcg
    11041 ggtggtaggc cacgtacggc atggacaccg aagcacccgg cgcgaagcgc aacacgccgc
    11101 agcacggatc ggccgcgccg ctgggcgtcc agggcgcgcc gacgccggcc cagcagtggt
    11161 tgttgtccac gtgcagggtc agcaccagac tgttgccctg caccaggctg ccgccgccag
    11221 gctgcgtcac ggagctggcc ttgacggtcg ggatcgagcc gctcaggctc acactgtcgg
    11281 gcacgtagta gtcgatgccc ttggcggcga tgtcgatctg gttgccggcc gcgtcgtaca
    11341 gatcgagctt gatttggtag aggccgctct catcgacgct gccgggcacc acatcgccgt
    11401 cttcttcgaa cgccagcccg cgcgagaaca cccggctgtc gaactctccg ttctcggtgt
    11461 ccagcacggc attggctatg gtccactggc caaacggcgg cagtgccggt ggaatctcgt
    11521 acagctccag ggtctgatcg cccaccacca tgtggttggg ccccagccgg taggggtcga
    11581 tcaccaggtc ggtgccgacc atgtgcgcat agtggcggtt gacctcgacg ctgagcggct
    11641 tgaagtcacc ggccatgccc ttgcgccagg acagctggta gtagcgcaca cccagcgatt
    11701 cgcgcagcgc gttatcgaag tccagccgcg gccgcagcac gccgccccag ggcgcgccac
    11761 tctgcagcag acccaggttg tcgcccgtcg caccgggggc cccgccgccg tggatggctt
    11821 tcagcgaggt gttgccgacc gccgtgaaca gcacccagtt gtcgcccggg tggtagggcc
    11881 ggcagggcga gcaggcgatg gcccaggggc tggtggtgat cagcgtcacc ggcgtgccac
    11941 agacgtagtt ccagtgcgtg tggcagggca ccggggtggg ctcgtagatg ctcacgttca
    12001 gccagccgaa caggcgctgc tgggccgtga agtacaggtc aggcacatcg ctgctctggc
    12061 agccgcgcca gaacacggcg cggaactggc cacattcgtc ggtcaccgcg gtggccacct
    12121 tctgcttggt gacgatgtgc ggatagaacc agcacagcag cggccgcacc agctcggggt
    12181 gacggatcag cgcctgctga aactgctgcg cgttgccgat ctgcgccagg gtcttcagct
    12241 cgccggcctg tggcgcggca tgaaagctcg ccagcacggc ggcgccgctg ccgatgccgg
    12301 gggtgcgccc ggcacgcatg gcgcggcgct gtgccagtgg caccgggtcc ggtccagggc
    12361 cgggcggcgg caggggcccc ggcccgtcga tcggccacgg tccgggcggc tccggccagg
    12421 gcgggcgcgg ctcgacgatc accctgcgca gcccgtcgat gacccagggc ggcagccgcg
    12481 gcacgagcac cggccaggga tcgacctccc acacgtcgac gctggcgtga cacaccggca
    12541 gttccagcgg cagaccgccg ctgaccacgc gcttgagcaa ggtcccgcgc accacgcagc
    12601 ggcccagcag ccagcagcgc cagctgtccg gatcgatcgg cagcaccagc ttcgggcgcg
    12661 ggtccttgag cgacaaccgc agtggccgcg ccaccgcgcc gcggcgcagc aactcgctgg
    12721 cctcgggccg ctccagcgtg ggccccacca gcacccgcac cgccgtgtcc tcggcgccga
    12781 cggccagcgc cagccgcgcc tggccgcgtt catccgccgg ctgcatggcg agcagcttgc
    12841 cgccggccga ataggcatag accgccggtt ggggggcggc ctgccgatcg tccacctcct
    12901 gcagctgcac atggaccgac agctcgacct tgcccgagcc gcccgcgttg tcaggatttc
    12961 tggccatgat gttctccttc cgttgcgttc accgtccaac ccttgccggc gccggcggcg
    13021 cacgggccgc cggccgccgc gggactcaat cgtcgtcgtc ctcggcctcg ccgaccgggc
    13081 agcagcggta ttgctgcgcc tgatcgagca gctcgatctc gcgcttgagc ttctcgttgt
    13141 ccagccgctt gcgttcgagt tcgagctcga tctcacgctg cagcgccggc tcgcaggtgg
    13201 cgcagtcgtc caggcacccc ttgaccagga tgcccggggt gggcagcacc tcttcgcgtt
    13261 cccaggacag ctcggccacc agcttgtcat gcacctggcc ggtcttggga tcgatcacgc
    13321 cggccgcagc cagctccttg tccaccgccg ccagcgcggc ctggcgcacg tccacaccca
    13381 gcggcgcggc ggcgccgacc agcgggcgcg ccagcagcga cacgttgccg ccggtcatgt
    13441 tcaggcgcga cagcgcgctg gcgcgggccc gctcctcgat cgccaggcgc tgcgggctgc
    13501 tggcgctgac cacctcgggc agcaccgaga tgcggccggc cacgtccacc gagggccgct
    13561 gatcgctcag caccggcgcc gccggatcgc tgaccacgcg ctggatcgcc accagccgga
    13621 agcgcagctt ctgaaccttg ctcacccggt agaacaggta ggtgacggcg tggcagcgat
    13681 tggggttgcg gaacatgcgc gaagccgatt cgaagtggtc ggacgattcg ccctccctgt
    13741 gctcgcgcgt gctgacttcg ccgaccgccg tgctgctctt ggcccgcacg ccggccgcca
    13801 ccgaagagga cgccgattgc gcgtgttgcg acagaaaacg cgcaaagctg ctggccgaat
    13861 gcgcgtcgta gctgccaccg ccgccgccgc cgccaatctc gatgatgccg aacaggttga
    13921 cgctggcacc gccaccgcct tcggcccagg actcgtcgta gctcgacgaa gccgagccgc
    13981 cttcggtgat ggtcaagtcg ctcaccgcac gcgccatgcc gtaggtgaag aacgattctt
    14041 cggacgtggt ttcatgccga taggccagct tggactcggc gtcgtacgac cagcgcgtat
    14101 ggcggtcgct ggaaaacagc cgcacctgct cgcccggcag cagcgtggtc gagtacacgg
    14161 gatcgcccag caccagcggg ccggagcagc gctccagacg gaaatgcacg atcagatcga
    14221 cccgcaccgt ctgccgatcg cccgtgcgca ccgggtaggg caatcgatag cgaaagtcga
    14281 gccggtcaca caccggttga cgttcgagca ccgggcagca ctcgatcctt gcaagggagc
    14341 tttctttcgt gtcgggcatg atggtctcct taaatgccgt ggcggacatg agcgacagca
    14401 gaccggaccc tgacagcttg ttgttgtgag acgcggaagc cgcctccagc atcggctgag
    14461 gcgggccatg ccggttagca ccgaattgcg cgggagtgca acgatcctgc ggccagagcg
    14521 gccagccgct atgccttgcc ctgcggcggc gccttcagcc gctgatactg cgtggctggc
    14581 acgcccacca ggcgccgaaa cacgcggccg aagtacgaca ggtcgttgaa gcccaccgca
    14641 taggccacct gcgacaccgt ggcaccgggc tcgcgcagga actcgcgcgc catggtcacg
    14701 cgatggtgct gcaggaagtg acagaacggc acgccctgct cgcgatgaaa ggcgcggctg
    14761 aactccgacg gcgacaggtg gcacagctcg gccatcagct tgatcggaca cggctcgccg
    14821 tagtgttcgc cgatccaggc cacggcggcg gcggtgcgcg cgctggcctg cagcgacttg
    14881 cgcaggtggc ccgccggctc acgcaactcc ggcggcaacg ccggcgccgg ccagccaccg
    14941 ggggcgcgcg cctcggcgcc gccgcagcgc agcacctcga acagacgccg cagcgtgctg
    15001 acggtgaccg gcttgacccg gtagtcccag acccggtgtc gcagggccca cacggccagc
    15061 gcctcggtgt ggtagtcggt gaacatcagc accggcagct gcgggaactc gcgccgcacg
    15121 cgcggcacgg cgtccaggcg cgcgccgtcg gggtagtcgt agtcgatgca gacgaagtgc
    15181 ggccgccagc gcgcgatggc gccaacgatg tcggccagct gcgtgatgcg ggccatgacg
    15241 ccatgcggcc gggcatcctg gtacagccca tgcggctgtg gcatgcagcg cagatcgatc
    15301 cacaccgcgc gcggcgcggg cgcaggcatg ggatcgatgc gactgtcggc caagggctgc
    15361 tcctgtagga ccgggcgcct gacccggcgc ttgcctggac gggccgggtc gtcagcgacg
    15421 cagcctgagc cggatgttag gaagcatcct catcggtgta tgtattaggt acacaagact
    15481 cgtatcaagt tacgtctttg gaagtaatct gatcgcgccc cagggtcacc atggttcggg
    15541 gcgcaaagcc atggttcagc ggccccacgc cctcgccggt ctgcaccgtg gcaccggccg
    15601 ccagcgcctg ctgcacgtag tggcgggcgg cttgcaccgc gtcgctcagg cccaggccca
    15661 gcgccaggtg ggcggcgatg gccgacgaca gtgtgcagcc ggccccgtgc aggttgcgcg
    15721 tggccagacg cgcagagcac atgtgcagcg gctcggcgcc gcgcaccagc agcaggtcat
    15781 gcacctgctg gcctttcagg tggccgccct tgagcagcac ggcgcgtgcg cccagcgcca
    15841 gcagcgcctg cgccgccggc gccatgtcgg ccaccgcctg caccggccgg cccagcagca
    15901 gcgccgcttc gtccaggttg ggggtgatca ggctggcgcg cggaaacagc gcggtcacca
    15961 gcgcctgcac ggcctcggcc tcgatcagcc gcgcaccggt ggccgacacc atcaccgggt
    16021 ccagcaccac ctgggtcatg tggtggcgct cgatggcggc ggccacggtg tgcacgatgt
    16081 cgggcgcgtg cagcatgccg atcttcaccg cctgcgcgcc gatgtcgttc agcacggcgt
    16141 cgatctgggc ggcaagcatg tccggcggca cgccgtggat ggccgtcacg ccctgggtgt
    16201 tctgcgccgt cagcgcggtg atcgcgctca tgccgtagca gcccagggcg gcaaaggttt
    16261 tcaggtcggc ctggatgccg gcgccgccgc ccgagtcgct gccggcgatg gtgagcacgc
    16321 gcacgtattg ctgcgtcatg gttttcacag tcaaaatggg ctgcagcgcc gatctgcaaa
    16381 gcgcaggcag ctatggattc ggaagtgttg ggtgcggcag cgccggcacc ggcaggcgcg
    16441 gcgcggcgcg gccggcggcg atggcggcct gccacacggg cagggtggcg gccgggtcgg
    16501 cgcccaggcc acgcaccacg cacacgccgg cggcgccgct tcgcgtcgcc tgcgtggcct
    16561 gggcgggctc caggatgccg ccgatcgcca ccaccggcac accggccatg tgcacccacc
    16621 agctcaggtt gtccaggccc tgggcgcgcc agggcatggc cttggtcagg gtcggccaga
    16681 ccgggccgca ggcgacgtac catggcgcca ggctgcgcgc gcgcgccagc tcccacaggc
    16741 tgtggctgct gatgcccagc ggcaggccgc aggcttgcag ctcggcgcgg ccggcgtcgc
    16801 ccagggccag caggtcttcc tggcccaggt gcacggcgat gccgcgcgcc gcgccggccg
    16861 tcagttcggc ggcgagccgc cagtggtcgt tgacgaccag ggtggcgccg gcctcgcggc
    16921 aggcgccgag cgcctgccgg atggcgcagc gcaattcgtc ctgccaggcg gcgggcggtg
    16981 cagcgggcgt cttgatgcgc aactgcaccg tgcgcgcgcc cgcggcaacg acctggcgca
    17041 cgcgctcggc gctgtcgacg atggcgtaca ggccaggcgc ctgggtttca agcggaatcg
    17101 ggctctggcg cgcacccaga aagcgccagc agctatccaa ttcggagcat gacagcagcg
    17161 gcatgcgcgc cggctcgacg gcaaagccgg ggcgcgccgc caccggcccg gcgccctgcc
    17221 cggccgcgtg accgtgggcg atggcgtggg cggtggccat cttggccagc accagcgcgt
    17281 cggccgccac gaagcccagc gccagcgccg ccgcggcgct gctggcaaag gtgcagccgg
    17341 tgccgtgcgt gtgccgggat tcgaggcgcg gcagcgccag ccagccacgg gcgtgctcgg
    17401 tatcgatcca gtccagcgcc cagcccggcg cgtgcacgcg gtcgccgccg gtgatggcga
    17461 ccgcctgcgc gccctgcgcg cgcagggcca cggccagcgc cggcacgtcg ctcggctcgc
    17521 cgggctggcc caccacccca gccctctgcc ccgaggggcg cgggttcggc ggtgcgccac
    17581 ccagcagccg ccgcgcctcg tgctcgttgg gcgtgaccag cgtggcgcgc ggcagcagca
    17641 gctcgcggta ggcgcgcagc acggcctcgt cggcaaaggc ggcgccgctg ctggccccca
    17701 gcaccggatc gaccaccagc gccagcggcg cccgctggcg cagccggtcg acccagcgcg
    17761 ccagcacggc cacgttgtcg gcgctgccca gcaacccggt cttgatggcg gccggcggca
    17821 ggtccgccgc cagtgccgcc agttgcgcgt cgagcaggtc ggccgccacc ggctcgacgc
    17881 gcgtcacggc ctgcgagtgc tgcgccgtga tggccgccac caccgggcac agatgcacgc
    17941 cgaacgccgc cgccgcgcgc tggtcggcgg caaggcccgc gccgccgccc gagtcgttgc
    18001 cggcgacact ccagacgatc ggttgagcta ctgattttgt agctgctggc gcttgtccca
    18061 tgggcgtcat ggcgcatttc cgatcagaaa cgggttgccg gtgacgggcg tggtggcgac
    18121 cgcgaaatcc tgccgcgcca ttaccccggc acgataggcg gcgcgcccgg cctcgatcgc
    18181 caggcggaag gcgccggcca tgcgcaccgg atcgggcgac tggctgacgg cgctgttgag
    18241 cagcacggcg tcaaacccca tctccagcgc ctgcgcggcg tggctgggcg cgcccaggcc
    18301 ggcgtcgatg atcagcacca cgtccggcag gcgctcacgc agcaggcgca gcgcgaaccg
    18361 gttgaccagg ccctggcccg atccgatcgg cgcgccccag ggcatcagca gcgggcagcc
    18421 ggcgtccagc aggcgccggg cggtcaccag gtcctcggtg cagtaaggga acaccgtgaa
    18481 gccgtcgcgc gtgagctggc gcgccgcctc gaccagctcc tgcgggtcgg gctgcagggt
    18541 gtagtcgtcg ccgatcacct ccagcttgac ccacgccgtg ccatacagct cgcgcgccat
    18601 gtgggccagc tgaatcgcct cgcgcgccgt ggtgcagccg gcggtgttgg gcagcaggcg
    18661 cgcgccctgc gtgcgcatgg cggcgcgaat gatctcgacg aagctgttgt cgccgccggc
    18721 ggccagctgg cgcttcaggc cgacggtgac gatctgcgtg cccgaggccg cgatggcgtc
    18781 ggccagcacc cggggcgacg ggtacttggc cgtgcccagc agaaagcggc tgtccagccg
    18841 cacgtcgccc accgcccagg tgtcgtggtc actgtcatgc atatcggtcc atccttggtg
    18901 cttgtccgca agttctcaga gactcagcca gccgcgctgc agacccagcg ccgccacacc
    18961 ggcggccgcc agcatggcca gaaagcccag ccactcgcgg cgcgagaaca ctgcccggcc
    19021 gtgcgagcgc cgcgcccagg cgtacagcag tgcgccgggc gcgtacagca acgcgcccag
    19081 caacaggtac ttgatgccgc ccgcatacac cagccacagc gcataggcca gcgccagcag
    19141 cgacagcagc gcgtcgcgcc ggcgccggcc gccttcggcc acgccgtagc gcgcgccact
    19201 gagcgcgatc ttcagcgcat ggccggcggc ccaggtgtag ggcagcagaa tcatcgccgt
    19261 gcccaggttc accagcgaca ggtaggtcga attgctccac agcgtcagca gcagaaaggc
    19321 ctgcacgcag gcgctggtca gccacagcgc gtgagacggc acgccgcgcg cgttctgacc
    19381 ggccagccag tccggcgccg cgccgtcgcg cgcggccgaa taaagcgtct cggccgacag
    19441 catgagccag gacagcagcg cccccgacag cgaaatcagc agcccgacgc tgatcagcac
    19501 cccgccccag tgcccgaccg cgcgctccag cacataggcc atcgacgggt tcttcaagcc
    19561 ggccagctcg ggctgcgcca tcacgcccat cgacagcagg ctcaccgcca ccagcagcgc
    19621 cagcacgaac agaaagcccg ccagcgtggc acgcccgacg tcgatgcgtc gctgcgcgtg
    19681 ggccgaatac acgctggcgc cttcgacacc gatgaacacc cacaccgtca ccagcatcat
    19741 gccgcgcacc tgctccagca cgctgcccag ctgcgggttg gcacggcccc acacatcggc
    19801 ccggaacacc tggggctgaa aggccagcgc caccagcagc acgaacagca gcagcggcac
    19861 cagcttggcc accgtcgtca cctggttgat cagcgccgcc tcgcggatgc cacgcaacac
    19921 cagcgcatgc gtgccccaca gcatgaccga ggcgcagacc accgccagcg gcgtgttgcc
    19981 gtcgccaaac accggccagt aatgccccag ggtggaaaac agcagcacgt agtagcccac
    20041 gttgcccagc accgccatga accagtagcc ccaggccgag ctgaagccgg tgaacggccc
    20101 gaagctggcg cgcgcgtagg cgtagatgcc cgaatcgagt tcgggctcgc gcagcgccag
    20161 gttcaggaac acgaaggcca gcgccagcat gcccacgccg gtgatcagcc aggcgatcgc
    20221 cgtggcaccg acaccggcgc tggccgcggt gttctgcggg atcgaaaaaa tgcccccgcc
    20281 caccatcgag cccaccacca gggcggtgag tgcgcccacg cgcagcttgg tcgtcttcgg
    20341 cggattcatg cagaccagcc ggtatcagcc gccgacgatg ggctggaaca gcagcacctg
    20401 atcgccctcg gccagccgct gcgtggcgcg cgccggtcga gccacgaagg caccgttgac
    20461 cgcggtgctg acggcctcgg gcgcgtgccc cagcgacgcc accacgtcgg ccagcgtgct
    20521 gccggccgga atggcatgtg gatgaccgtc gagcaggatg tgcagcgtgg cggcgtcgga
    20581 tgaatggctg ggcatggggg tcggcgggtt tcagacatcc ttgcgcagag gacgcaaggc
    20641 ttgcgcagaa gtcgcaaacc gcgctttctg gcgcatctgc gaagtctgcg aattctgcgc
    20701 aagctttgcg gcttctgcgt acgcgcgggg tgttttcatg acacgctcat caggcctgcg
    20761 cctttgccgc accgtggcgg gacggtcgcc catcggacgc caacgcggta tccagccagg
    20821 gcgcctcggc cagcgcgcgc tcgaccagcg ccggcgccag cagccagccg tggcgaaaca
    20881 gtccgttgat gcgcagcagc cggccggcgt gctcgatgag cgggttgttg tcgggcagcg
    20941 ccgggcgcag gttgacgtcg agtttgagga tgcgcgcctc ggccagaccg ggcagcacgc
    21001 tgtgcgcggc ggccatcagt tcgaccgcgg tgcgcaggct gaccggcgag cggtcttcgc
    21061 tttcgatttc gctcgcaccg accaccacgt cgcgcgacga gcgcggcacc atgtagacga
    21121 agtggcgcgg atgcagcagg cgcgccgggc gcgtcaggcc atggcccggg cagtccagcc
    21181 acaccacttc gccgcgcacg ccgcgcaccg gcaactcggg ccgcgcaccg gcaccccgca
    21241 cgtcgaccac cgcgtcgaac gccagcgcgc gaccgtcggc cagcagcagc gtgccgccag
    21301 cgtcgccgcc ctgcagcgcc cgcaccggca cgccccagtg ccagtccacg ccggacgcac
    21361 cggcgtgcag ggcgatcatg gtcgccacgg tgtcgatgtg gccctcgccc ggcagcagcc
    21421 aggcatgggc cgggccctga atggcgggct ccagcgtacg cagctcggcc ggcgacagcg
    21481 gctgctcgcc cgccggcagc tgcaggtggc gccattcggg cgttgcggtg gcgacgtgca
    21541 gccgatccag catgcgccgg gcggtgccca gatcggcacg gtgcgccagc agcaggctgc
    21601 cgcgcaccac caattcaggg gcgcccggca gcgccgccag gatgcccggc cacagcgcca
    21661 gcgagcgaaa gcccatgtgc gccaccgccg gctcggcgtt gtccagctcg gacagcggac
    21721 tgagcatgcc ggccgccgtg aagccggcgg ccgtgggcac ataaggatcg ccggcctggc
    21781 tgcgcgccac cggctgtggc ccggcggcgc tgtcgaacac cgacacctga tggccgcggc
    21841 gcgacagctg ccaggccagc aatcgcccca gcaggccggc gccggcgatg ccgatggaaa
    21901 gcgcgggcgg gctcatgatg gacgtgcccc tggcgtgccg cggcacacgg ccgacgccgg
    21961 cgcgcgccgc caccagggag cgatcagagc ggagggatgc atcgggcggt ctcctgcaag
    22021 ggccacagac aatcgggctc cggggccgcc tgcgccgggt tgcatccgag ggatggcgtg
    22081 cggcgcgctg ctgctcttcc ttacgccggt gctaaccgga tcaagttgac gggttcgact
    22141 tgctgccaag ccgtctcagc tcgctcgcgc gggcaccccg gagttgcgac cgataatagc
    22201 cggaaaggag gcgccagcca tgacccacgt cagaactctg ctcaccctgg ccctgcccgg
    22261 cgcactgctg ctgggcgcct gcgccggcgg cggccatccg cgcccaggca ccagcacgca
    22321 gcaggagtac gaagcggcga tgcgcgacca atcaccggtg cgcgtgatcg tgcgcgaagg
    22381 cccgatttcc aacagcgacg ttggtcgcac cccggaagag ctggcccgcc aggaagccgg
    22441 ccagcagtgc ggccgcgcca gcctggacgc caaggtgctg gacgtgcagc gccagcgcgc
    22501 gcccgggcgc gacgccaccg gcggcgtggt ccagttcgag cgcgtgcgcc tgaccttccg
    22561 ctgcgagcgc ggccgcccgt gagtgtggtt ttgcgagcca tgcgcaccgg ggggccggcg
    22621 cagtcgccaa aatccggtat ataatttgag gcttgttccc cgatagctca gtcggtagag
    22681 cgccggactg ttaatccgta ggtccctggt
//
LOCUS       DAOQTZ010000038        22555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2689_length_22555_cov_12.907600, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000038 DAOQTZ010000000
VERSION     DAOQTZ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 22555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22555
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2689_length_22555_cov_12.907600"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..470)
                     /locus_tag="PKN51_06365"
     CDS             complement(57..470)
                     /locus_tag="PKN51_06365"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HNT84759.1"
                     /translation="MGPYTAPTGTWVTETLGDSTLMWVWQSGAANSQPPVTTMAQFKA
                     GSYVPESGGFTIPATATILGVQVGTGSGWYGVFRDAVDTIGLAAQSSLGPDNFELTAP
                     PPAAVAPVPTLDGLGLSALAGLLAGLGLWRQRRRG"
     gene            complement(481..777)
                     /locus_tag="PKN51_06370"
     CDS             complement(481..777)
                     /locus_tag="PKN51_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84760.1"
                     /translation="MWQRIDIRATASVGITGDYPRNGDGSVLMTSGDGTGKTTWQHFP
                     AGGLGPLSSFISATYEWYRDAASTVTRPLSSGVPHSGGQRRQPRHHGRPVLAGL"
     gene            complement(1060..1539)
                     /gene="trmL"
                     /locus_tag="PKN51_06375"
     CDS             complement(1060..1539)
                     /gene="trmL"
                     /locus_tag="PKN51_06375"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNT84761.1"
                     /translation="MFHIVLVEPEIPPNTGNVIRLAANTGCTLHLIEPLGFSMDDRQL
                     RRAGLDYHEYAPVRRHAGWEAFVASERPAAERLFALTTRGTRGVHEVSFAAGDWLVFG
                     AETRGLPAALRERLPAAQQLRLPMRPGQRSLNLSNAVAVTVFEAWRQNGFGGAGAPV"
     gene            complement(1562..2293)
                     /locus_tag="PKN51_06380"
     CDS             complement(1562..2293)
                     /locus_tag="PKN51_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNT84762.1"
                     /translation="MRPMTLLRAWLRHPAGASAWPSQCLICRRWPAQTLCAECRARFA
                     PQLTRCQRCALAVPAGVAVCGACLREPPPMAQCLAAVSWQWPWSMCIGRWKFEGDTGL
                     SGPLAALLQATPGVAEALAAAELLLPMPMSDQRLAERGYNPALLLARRLAPGRVQPGW
                     LLRTRHTPPQRGLPRAERLRNVRGAFQVDPLAAGRLRGRRVLLVDDVMTTGASVREAA
                     ATLRAAGADPVGVLVLARTDEPTTH"
     gene            2407..3330
                     /locus_tag="PKN51_06385"
     CDS             2407..3330
                     /locus_tag="PKN51_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HNT84763.1"
                     /translation="MSPAASDRPPSIDPVAARRWAERQHHGSPWLHEEVARRMAERLA
                     VIRLPVRAWAHWEPVRGGLQGHERVAAQYPDAECFRVSSQYPSAPEATKNIASGWWQR
                     LTGGRPSAHDVARPPEASVQLVWANMLAHQVAEPAALMADWLRALAVDGFVMFSCLGP
                     DTLRELRALYADLGWPPPAHEFTDLHDWGDQLLAAGFADPVMDMERITLSFATPQRLL
                     QELRGLGRNLHVQRAAGLRTPAWRAQLQAALARHPALQAGDGALTLSFEVIYGHAVKP
                     PPRHTIAPQTRISLDDLKVSLRQRNRNLPSD"
     gene            3421..3909
                     /locus_tag="PKN51_06390"
     CDS             3421..3909
                     /locus_tag="PKN51_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HNT84764.1"
                     /translation="MQQASTQRWAHVGDQALAWRLRRHCSVTPRQLAGLYAAMCLLSL
                     SIAVGFWALGMPLVLAFAGLELLAVGAALLVHARHALDGETVHLQGGRLIVERECAGR
                     CERLEFGAAQVRIAPPASERGLIELSAAGRRIAIGRHVRPSQRPALASDLRKALWAGG
                     LH"
     gene            3966..5150
                     /gene="coxB"
                     /locus_tag="PKN51_06395"
     CDS             3966..5150
                     /gene="coxB"
                     /locus_tag="PKN51_06395"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNT84765.1"
                     /translation="MASKLTGRASQVALWGALWLAGAAQAQVNDLPGGPAVRQLNLHP
                     PVTKIAEAQHSLHWMLLIVCTIIFIGVFGVMFYSIWKHRKSQGAKPAQFHESVAIEVT
                     WTVVPFLIVIGMALPATKVVVAQKDTTNADLTIKATGYQWKWGYDYLNGEGAGIGFLS
                     TLDASHRVMSDAGKPAGDDYLLKVDHPLVVPVGKKVRIITTANDVIHSWMVPAFGVKQ
                     DAIPGFVRDTWFRAEQTGDFYGQCAELCGKEHAYMPIHVKVLPQHEYTAWVEQERKRL
                     AALADDPNKSWTLAELVARGEKVYAANCAACHLENGRGSAAIKPLDGSAIVKGPAAEQ
                     MRVVLEGRANGTMPAWKQLSDVELAAVVTYTRNHWTNQTGTLVQPAEFKAARTGKFPA
                     GG"
     gene            5209..6837
                     /gene="ctaD"
                     /locus_tag="PKN51_06400"
     CDS             5209..6837
                     /gene="ctaD"
                     /locus_tag="PKN51_06400"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HNT84766.1"
                     /translation="MSAVLPTPHAGHDDHAHDHHHAPTGWRRWVYATNHKDIGTLYML
                     FSFFMLMVGGVLALLIRAELFQPGLQVVNPELFNQLTTMHGLIMVFGAIMPAFVGFAN
                     WMIPLQIGASDMAFARMNNFSFWLLIPAAIMLVGSFFMPGGAPAAGWTLYAPLTLQMG
                     MSMDSSILAMHIMGASSIMGAINIIVTILNMRAPGLTLMKMPMFCWTWLITAYLLIAV
                     MPVLAGAITMTLTDRHFGTSFFNPAGGGDPVMYQHIFWFFGHPEVYIMILPAFGIISH
                     VVPAFARKKLFGYASMVYAVAAIAILSLIVWAHHMFTTGMPVTGQLFFMYGTMLIAIP
                     TGVKIFNWVATMWRGSMTFETPMLWAVGFIFVFTIGGFTGVIPAVVPVDTQIQDTYYI
                     IAHFHYVLVAGSLFALYGGFYYWSPKWTGVMYNETRGKIHFWWSMISFNVTFFPMHFL
                     GLAGMPRRYADYPMQFADFNLIASVGAFFFGFAQLYFFLFVVLPVMRGQGAKAPQRPW
                     EGAEGLEWEVPSPAPFHTYETPPKLDASATRVIG"
     gene            6864..6977
                     /locus_tag="PKN51_06405"
     CDS             6864..6977
                     /locus_tag="PKN51_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HNT84767.1"
                     /translation="MTTEQQRKRNVRLGLILGTVALAFFVGFIVRMVVLGG"
     gene            6993..7610
                     /locus_tag="PKN51_06410"
     CDS             6993..7610
                     /locus_tag="PKN51_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNT84768.1"
                     /translation="MRRRSENLKMLGKLAVVACGMFAFGYSLIPVYRAICEATGINIL
                     SLSERQVPGNGSAGETARLASRNTQVDRSRVITVEFDANTRGPWEFRPAQRTLQVHPG
                     ELATVMYEFQNVQDRRMAAQAIPSYAPRQAAPHFNKLECFCFNQYTLEPGEKKEWPVA
                     FVIDPRLPKDVTTITLSYTFFEVGGKTPAPPQSSAALARPHGAGA"
     gene            7607..7882
                     /locus_tag="PKN51_06415"
     CDS             7607..7882
                     /locus_tag="PKN51_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HNT84769.1"
                     /translation="MSEPARTVQTPVSPAPPDVAAPAAAGGPAFWRTLRMVGWGFLGV
                     RKRSEYQRDLAQVNPFHIMLAGLIGVVLFVLLLLGIIHWVVSGTTPG"
     gene            7972..8853
                     /locus_tag="PKN51_06420"
     CDS             7972..8853
                     /locus_tag="PKN51_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNT84770.1"
                     /translation="MSSTTHGQTPYYYVPAESRHPVMAAFGLLLMIIGASQWINDAAW
                     GGWLLLAGLLWWLFVLFQWFSDAVGESEGGLYGRKIDLSYRWSMSWFIFSEVMFFGAF
                     FTALWWARSHSVPALGSLDNALLWPDFKAVWPSVAPGATASPAGTVQPFQTMGPFWLP
                     TINTALLLTSGVTLTIAHHALLAGQRARTVAFMWVTVLLGAIFLCVQGYEYYHAYTDL
                     NLKLSSGVYGSTFFMLTGFHGLHVLVGMLMLLFITLRLMKGHFTPQRHFGFEGAAWYW
                     HFVDVVWLGLYLLVYWM"
     gene            complement(9040..9276)
                     /locus_tag="PKN51_06425"
     CDS             complement(9040..9276)
                     /locus_tag="PKN51_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HNT84771.1"
                     /translation="MKQVFVALAFVGILASLASALVFMLRRDPQGEATDSRRAGRMMR
                     SLALRVGLSVLLFLCILLAWKLGYIQPSGISSSF"
     gene            9292..10020
                     /locus_tag="PKN51_06430"
     CDS             9292..10020
                     /locus_tag="PKN51_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNT84772.1"
                     /translation="MSRWRSLLVLLAALLGVGLTFALGRWQLARAAEKQALHAAIEAR
                     QSLPALDGLTLAGPLAPDQAQALLHRRITLRGRWLPQRTVYLDNRPMGRQTGFYVLTP
                     LRLSGSDAVVLVQRGWAPRHRSERETLPPVQTPDGEVQVHGRVAEHPPRLYQLGQEGL
                     GAIRQNLDLDAYRAETGLPLAGLMVWQTGAPSEGLRRDWPEAASGVDKHYGYAFQWFG
                     LCALIGLLFVWFQVVRPIYRTRRA"
     gene            10049..10699
                     /locus_tag="PKN51_06435"
     CDS             10049..10699
                     /locus_tag="PKN51_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84773.1"
                     /translation="MTVHNLPSPEALLAQQARRTRAGRWQMLLLALVCAAPVIASYYS
                     YYVVRPEARRSFGELIEPQRPLPVASATDLSGRPAALSALKGQWLLISVGGGACDAVC
                     ENHLYLQRQLREGLGKDKDRLDWVWLVSDDARLPEPLLPALGQATVLRVPPDVLGGWL
                     APAPGRALPDHLYVVDPMGHWMMRFPAGLDKQSAGRAKRDLERLMRASSSWDRAGR"
     gene            10696..11832
                     /locus_tag="PKN51_06440"
     CDS             10696..11832
                     /locus_tag="PKN51_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNT84774.1"
                     /translation="MMDATPLYNLSPALRLMGMGLVVALGPLAWLWLRHQGAGPARRL
                     QALTLLTLFLTFDLVMFGAFTRLTDSGLGCPDWPGCYGQASPVGARVQIDAAEAAMPT
                     GPVTHGKAWVEMIHRYLATGVGVLILVLTLATWRQWRRQRAAAATDIVHPGWPLLTLL
                     WVCAQGAFGALTVTMKLFPAIVTLHLLGGVGLLVLLVVQAESYRRSAGAAPEPLAAGL
                     RALLWLSAGAVALQIALGGWVSTNYAVLVCNTFPMCQGSWWPPMDFAQGFELWRPLGE
                     SADGSRLEFHALTAIHYTHRLFAYAVFLLLGLLAWRLARAGRLAAQRRWVLGLAALQL
                     LTGLSNVILDWPLLAAVLHTGGAAALMTVLTWALCASHRPELSR"
     gene            11829..12743
                     /gene="cyoE"
                     /locus_tag="PKN51_06445"
     CDS             11829..12743
                     /gene="cyoE"
                     /locus_tag="PKN51_06445"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNT84775.1"
                     /translation="MSAPGTAAVLPSMPIWKQYYVLTKPRVVQLIVFCALIGMVLAVP
                     GLPDAAALQRMLIACVGIWLVAAAAAAFNCLVEKAIDARMRRTSWRPTARGELSAAQA
                     LGFSALLCALGSAVLYLWVNPLTMWLTFATFVGYAIIYTVILKPLTPQNIVIGGASGA
                     MPPVLGWAAMAGTVEPQALILFLIIFLWTPPHFWALALYRVEDYRKSGLPMLPVTHGN
                     EFTRLQVLLYTLILLAACLLPFIYRMSGWLYLVAALVLNAGFIGYAFQLWRRYSDALA
                     RKTFRFSLIHLSLLFAALLVDHYLPWTR"
     gene            12746..13357
                     /locus_tag="PKN51_06450"
     CDS             12746..13357
                     /locus_tag="PKN51_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNT84776.1"
                     /translation="MDRRLAIKNLAAGALLVSAWALLTACQPDRPNFHGIDITGVDYA
                     RDLRVRDFNGQPRTLADFRGKVVVIFFGYTQCPDVCPTTMGEMLRVKQLLGADGDKLQ
                     VLFLSLDPERDTPEVLKAYMGSFDPGFLGLYAGSADELAALAKDFKVFYKKVEGSTPG
                     SYTMDHTASSYIYDPQGRLRLVSRYGTRPEDVAADVRQLLGGA"
     gene            complement(13448..14443)
                     /locus_tag="PKN51_06455"
     CDS             complement(13448..14443)
                     /locus_tag="PKN51_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT84777.1"
                     /translation="MHPTFTRRRLLAGAAVCAGLLAPLTTSHAQTAAAWPSKPLRFIV
                     GFPAGSSPDLTARALAEPLEKALGQPVVVENRVGAGGNIGGDAVAKATDGHTIGLMIN
                     GNLTIARLLNPALRYDPATDFAPISLIGVAPLVLVAPASAPGSDARSFLDAARAAGSK
                     WSYGSPGVGTVGHLGMELLKARTGIAPVHVPYPGYPQVFNALVGGDLQLSMLPPALAQ
                     TQIKAGKLRGIGVTGSGRSPLAPELPSLLDAGVKQFNLEIWNAVVAPAGMPRAHVARL
                     AEAISTIVRAPEMRQRLFQQGWQAVGSSPEGLANRIQADTRVLGQIIRTQGIRVD"
     gene            complement(14749..15687)
                     /gene="rpoH"
                     /locus_tag="PKN51_06460"
     CDS             complement(14749..15687)
                     /gene="rpoH"
                     /locus_tag="PKN51_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNT84778.1"
                     /translation="MTQKVSSPSSNAVALTNPWSVVPPLGNLDAYISAVNRLPMLTLE
                     EEQRYARELRDTGSLEAAGRLVLSHLRLVVATSRQYLGYGLPHADLIQEGNVGLMKAV
                     KRFDPDQGVRLVSYALHWIKAEIHEYILKNWRLVKVATTKAQRKLFFNLRSMKQGFKA
                     ELDADLAHRDALTEEQIDVVARELNVKPDEVREMETRLAGGDVLLDPAPADDGEQAFG
                     PIAYLSDERHEPTAMLESAQRDVLATEGIATALRSLDERSRRIVTERWLNVNDDGSGG
                     MTLHELAAEYGVSAERIRQIEVAAMKKMRTALADYA"
     gene            15872..16318
                     /locus_tag="PKN51_06465"
     CDS             15872..16318
                     /locus_tag="PKN51_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84779.1"
                     /translation="MPLRSVGLVAALLLAGHAQAAHGYQGRVHGSFAGHAIDVPVICE
                     RPRLAGQRGNWFYAQSDPPTHEYAQDRNGDGVAVTVSFSGQEAMFMLYLAGQDHNFGN
                     TRREQIVLTEGGFVLTMSASRYEGKGRLRRKVGEDEIRLAVDCPQR"
     gene            complement(16405..17313)
                     /locus_tag="PKN51_06470"
     CDS             complement(16405..17313)
                     /locus_tag="PKN51_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNT84780.1"
                     /translation="MDLRQLEYFVRVAELGSFSRAALALDVAQPALSRQVRLLEVELR
                     QNLLTRHGRGAMPTEAGQVLLEHARGILHQIEHARDELARLRGGLAGRVTLGLPPSVA
                     RALAVPLVQAFGGQLPEAQLSITEALSASMQEQLASGRLDIAVLYNAQPVPGLDIAPL
                     ARDELLLVQHAGARADAAPATLRDLAQLPLVIPSRPNAIRMHVETQMAAAGCTPQVAL
                     EIDGVATILALVEGGAGGAILSRHALAGTAHPEAFALRRIATPDGQPLQIPLYTALSA
                     LRPASATRQAALTLLRAQALTHLGSF"
     gene            17456..18484
                     /locus_tag="PKN51_06475"
     CDS             17456..18484
                     /locus_tag="PKN51_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT84781.1"
                     /translation="MVIDCHGHYTTAPKALEQWRNRQIAGIQDPAAMPRVDELRISDD
                     ELRESIENNQLRLMRERGSDLTIFSPRASFMAHHIGDFQVSSTWAAICNELCHRVSQL
                     FPEHFIGAAMLPQSPGVDPASCIPELERCVRDYGFVGINLNPDPSGGHWTSPPLTDRH
                     WYPIYEKMVEWDIPAMIHVSTSCNACFHTTGAHYINADTTAVMQLIQGDLFKDFPTLR
                     FIIPHGGGAAPYHWGRYRGLAQELKKPLLQEHLLNNLFFDTCVYHQPGIDLLTKVIPV
                     DNILFASEMIGAVRGIDPETGHYFDDTRRYVQATANLNDEQRYKVYEGNARRVFPRLD
                     AALKARGR"
     gene            18488..19171
                     /gene="ligK"
                     /locus_tag="PKN51_06480"
     CDS             18488..19171
                     /gene="ligK"
                     /locus_tag="PKN51_06480"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HNT84782.1"
                     /translation="MYELGVVYRNIPRADGATADALAQFGSATVHEAMGRVGLLKPYM
                     RPIYPGAQVSGTAVTVLLQPGDNWMLHVAAEQIRPGDIVVAAVTAECTDGYFGDLLAT
                     SFQARGARALIIDAGVRDVRTLQQMGFPVWSKAISAKGCIKATLGSVNIPVVCAGMLV
                     TPGDVIVADDDGVVCVPAARAVQTLEAARKREANEGDKREIFASGTLGLDYYKMREGL
                     AKAGLRYID"
     gene            19182..20111
                     /locus_tag="PKN51_06485"
     CDS             19182..20111
                     /locus_tag="PKN51_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT84783.1"
                     /translation="MSKPTSGDFTKTAGWLDWYDGPSTPRFRLPAGSVDAHCHVFGPG
                     AEFPYAPERKYTPCDAGKAQLFALRDHLGFARNVIVQATCHGADNRALVDALQASGGR
                     ARGVATVRRSVTDDELQALHAAGVRGVRFNFVKRLVDFTPKDELMEIAGRIARLGWHV
                     VIYFEAVDLPELWDFFTALPTTVVVDHMGRPDVGKPVDGPEFALFCKFMREHANVWSK
                     VSCPERLSVSGPPALSGEQAAYRDVVPFARHIVESFPDRVLWGTDWPHPNLKNHMPDD
                     GLLVDFIPHIAPTPELQRKLLVDNPMRLYWPEA"
     gene            20265..20633
                     /gene="ligA"
                     /locus_tag="PKN51_06490"
                     /pseudo
     CDS             20265..20633
                     /gene="ligA"
                     /locus_tag="PKN51_06490"
                     /inference="COORDINATES: protein motif:HMM:NF009917.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
     gene            20769..21629
                     /locus_tag="PKN51_06495"
     CDS             20769..21629
                     /locus_tag="PKN51_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HNT84784.1"
                     /translation="MGARITASVYFSHVPAIGAAIDHGKTAEPYWAPMFQGFEFSKRW
                     MKEHLPDVIFLVYNDHATNFDLSVIPTFAIGTAAEFEPADEGYGPRPVPRVKGHPRLA
                     SHIAQSVIQQDFDLTIVNQLKVDHGLTVPMNLMFGTPAEWPCAVIPFHVNVVQYPVPS
                     GARCFALGQAIRRAIESYDEPLKVQIWGTGGMSHQLQGPRAGLINAEWDNRFLDRLIA
                     DPKGLSAMPHIDYVREAGSEGIELVMWLIARGAMADVAGGPAPKVAHRFFHVPASNTA
                     VGHLILENQA"
     gene            21685..>22555
                     /locus_tag="PKN51_06500"
     CDS             21685..>22555
                     /locus_tag="PKN51_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT84785.1"
                     /translation="MSKTINVALAGAGAFGIKHLDGIRNIDGVRVVSLVGRELDKTKE
                     VAAKYGISHVTTDLADSLALPELDAVILCTPTQMHAAQAMQCLQAGKHVQVEIPLCDK
                     LADGQQVAALQKQTGLVAMCGHTRRFNPSHQYVHQKIAAGEFHIQQMDVQTYFFRRTN
                     MNALGQPRSWTDHLLWHHAAHTVDLFAYQAGSPIVQANAIQGPIHKELGIAMDMSIQL
                     KAANGAICTLSLSFNNDGPLGTFFRYIGDSATYIARYDDLVNGKEEKIDVSQVAVSMN
                     GIELQDREFFAAIR"
BASE COUNT         3434 a         7730 c         7766 g         3625 t
ORIGIN      
        1 cccgcatcga tgcggcctgc ccgaagttga caagcggtca actgccgttt ctaggatcag
       61 ccgcgccggc gctggcgcca cagccccaga cccgccagca ggccggccag cgcgctcagg
      121 cccagcccgt cgagggtggg cacaggggca acggcggccg gtggcggtgc cgtcagttcg
      181 aagttgtcgg gccccaggct ggattgggcg gcgaggccaa tggtgtccac cgcgtctcgg
      241 aacacgccat accagcccga accggtgcca acctgaacgc ccaggatggt ggcggtcgct
      301 gggatggtga aacctcccga ctcggggacg tagctgccgg ccttgaattg cgccatggtc
      361 gtgacagggg gctgactgtt ggcggcgccg ctctgccaga cccacatcag ggtgctgtca
      421 ccgagggttt cggtcaccca ggtgccggta ggcgcggtgt aggggcccat ggcgccgcgc
      481 tcaaagacca gccaggactg gtctgccgtg gtggcggggt tgccgtcgtt gtccacccga
      541 atgcggaaca ccggatgaaa gtgggcgggt gacggtgctg gccgcatcgc ggtaccactc
      601 gtaggtggcc gaaatgaagc tgctcagcgg gcccaggcca cccgcgggaa agtgctgcca
      661 ggtggtcttg ccggtgccgt cgccggaggt catcagcacg gagccatcgc cattgcgcgg
      721 gtagtcgccc gtgatgccga ccgacgcggt ggcgcgaatg tcgatgcgct gccacacacc
      781 gaacgccggc gcatcgctga atggcccgtt gacgacgcct gccggcgtgg tgacgacggc
      841 cgcgctggcc gccccctgca gcgaccgcca gcgcgctggc caggagggtg cgccgaatcc
      901 gggttcggca ggttgatgtg gggttcatgg gcacccttca ctgtggtgaa cgatcagccg
      961 cgattgtcgc ggacgggatc gggaccaacg gcgttggtgg cgcagatacc ggcgtacttg
     1021 taacaaaaag caacacagcc cgcgcagccc atgcgtggtt cagacaggcg caccggcccc
     1081 gccaaagccg ttttgccgcc aggcctcgaa caccgtgacc gccaccgcgt tggacaggtt
     1141 caggctgcgc tggccgggcc gcatcggcag gcgcagctgc tgcgccgccg gcaggcgctc
     1201 gcgcagcgcc gcgggcaggc cgcgcgtttc ggcgccgaac accagccagt cgccggcggc
     1261 aaagctgacc tcgtgcacgc cgcgcgtgcc gcgcgtggtg agggcgaaca ggcgctcggc
     1321 ggcgggccgt tcgctggcca caaaggcctc ccagccggcg tggcggcgca ccggggcgta
     1381 ttcgtggtaa tcgagcccgg cgcggcgcag ctggcggtcg tccatcgaaa agcccagcgg
     1441 ctcgatcagg tgcagcgtgc agccggtgtt ggccgccagc cggatcacgt tgccggtgtt
     1501 gggcggaatc tcgggctcga ccagaacgat gtggaacatg gcgccaggct gtgtggaagg
     1561 ttcagtgcgt ggtcggctcg tcggtgcgcg ccagcaccag cacgccgacc gggtcggcgc
     1621 cggccgcgcg cagcgtggcg gcggcctcgc gcacgctggc gccggtggtc atgacgtcgt
     1681 ccaccagcag cacgcggcgg ccgcgcaggc gcccggcggc cagcggatcg acctggaagg
     1741 ccccgcgcac gttgcgcagg cgttcggcgc gcggcaggcc gcgctgcggc ggcgtgtggc
     1801 gcgtgcgcag cagccacccc ggctgcaccc gcccgggcgc caggcggcgc gccagcagca
     1861 gggcggggtt gtagccgcgc tcggccaggc gctggtccga catcggcatc ggcagcagca
     1921 gctcggcggc ggcgagcgcc tcggctaccc ccggcgtggc ctgcagcagc gccgccagcg
     1981 ggccgctcag gccggtgtcg ccttcgaact tccagcgccc gatgcacatc gaccacggcc
     2041 attgccagga cacggcggcc aggcactggg ccatcggcgg cggctcacgc agacaggcgc
     2101 cgcacaccgc cacgcccgcc ggcaccgcca gcgcgcagcg ctggcagcgc gtgagctgcg
     2161 gagcaaagcg cgcgcggcat tcggcgcaca gcgtctgcgc cggccagcgg cggcagatca
     2221 ggcactggct gggccaggcc gaagcgccgg cggggtggcg cagccaggca cgcagcaggg
     2281 tcatggggcg catgggcggg gcttgtaaac gaattgggaa tgacgaatga ccgggcggcg
     2341 cggcgatgac cccgggcgcc atgggccatc tttttgcgcg ggagcgcatc aatatactgg
     2401 ccggccatgt cgcctgcggc ctctgaccga cctcccagca tcgaccccgt ggcggcgcgc
     2461 cgctgggccg aacggcagca ccacggctcg ccctggctgc acgaagaagt ggcgcggcgc
     2521 atggccgagc ggctggccgt gatccgcctg ccggtgcgcg cctgggcgca ctgggagccg
     2581 gtgcgcggtg gcctgcaggg gcatgagcgg gtcgcggcgc agtatccgga tgccgagtgt
     2641 ttcagggtct caagtcaata cccgtcagcg ccagaagcta ccaaaaatat agcatccggc
     2701 tggtggcagc ggctcaccgg cggccgcccg tcggcgcatg acgtggcccg gccgccggag
     2761 gcgtcggtgc agctggtgtg ggccaacatg ctggcgcacc aggtggccga gccggccgcg
     2821 ctgatggccg actggctgcg cgcgctggcg gtcgacggct tcgtcatgtt ctcctgtctg
     2881 gggcccgaca cgctgcgcga gctgcgtgcg ctgtacgccg acctcggctg gccgccgccg
     2941 gcgcacgaat tcaccgacct gcacgactgg ggcgatcagc tgctggcggc cggctttgcc
     3001 gatccggtga tggacatgga gcgcatcacg ctcagctttg ccaccccgca gcggctgctg
     3061 caggagctgc gcggcctggg gcgcaacctg catgtgcagc gcgcggccgg cctgcgcacg
     3121 ccggcctggc gggcgcagct gcaggcggcg ctggcgcgcc acccggcgct gcaggcaggc
     3181 gacggtgccc tgacgctgag tttcgaggtc atctatggcc atgccgtcaa gccaccgcca
     3241 cgccacacca tcgcgcccca gacccgcatt tcgctggacg acttaaaggt ttcattgcga
     3301 cagcgcaata ggaacttgcc ctctgattga ggtttatcaa agacctgcgg cgactcggtt
     3361 aaactcattt gcattcatag cgaagcgacg gcgctttgcg gtgatgcgga gaacctcgtc
     3421 ttgcagcaag cttcgaccca gcgatgggct cacgtcggtg accaggccct ggcctggcgg
     3481 ctgcggcgcc actgcagcgt gacgccgcgc cagttggctg ggctgtacgc cgcaatgtgc
     3541 ctgctgtcgc tgtcgattgc agtcggcttc tgggcgctgg gcatgccgct ggtgctggcg
     3601 tttgccgggc tggagctgct ggccgtcggc gccgcgctgc tggtgcatgc gcggcatgcg
     3661 ctggacggtg aaacggtgca cctgcagggc ggtcggctga tcgtcgagcg cgaatgcgcc
     3721 ggtcggtgcg aacggctgga attcggcgct gcgcaggtgc gcatcgcgcc gcccgccagc
     3781 gagcggggcc tgatcgagct gtcggcggcg ggccggcgca tcgccatcgg tcgccatgtg
     3841 cggccgtcgc agcgtccggc gctggccagc gacctgcgca aggcgctgtg ggccggtggg
     3901 ctgcattgag tgggacattt gaatctatgg tgggggcagt gaacaacgtg ggcacgatga
     3961 atcgcatggc aagcaagctg actggaaggg cgtcccaggt ggccctgtgg ggcgcgttgt
     4021 ggctggccgg cgccgcgcag gcgcaggtca acgacctgcc gggtggcccg gccgtgcggc
     4081 agctcaacct gcacccgccg gtgaccaaga ttgccgaggc ccagcacagc ctgcactgga
     4141 tgctgctgat cgtctgcacg atcatcttca tcggcgtgtt cggcgtgatg ttctattcca
     4201 tctggaagca ccgcaagtcg cagggcgcca agccggccca gttccacgag tcggtggcga
     4261 tcgaggtgac ctggacggtg gttccgttcc tgatcgtgat cggcatggcg ctgccggcca
     4321 ccaaggtggt ggtggcacag aaggacacca ccaacgccga cctcaccatc aaggccaccg
     4381 gctaccagtg gaagtggggc tacgactacc tgaacggcga aggcgcgggc atcggttttc
     4441 tgtccacgct cgacgcctcg caccgcgtca tgtccgacgc gggcaaaccc gccggtgacg
     4501 actacctgct gaaggtcgac cacccgctgg tggtgccggt gggcaagaag gtgcgcatca
     4561 tcaccaccgc caacgacgtc atccactcct ggatggtgcc ggccttcggc gtcaagcagg
     4621 acgccatccc cggcttcgtg cgcgacacct ggttccgcgc cgagcagacc ggcgacttct
     4681 atggtcagtg cgccgagctg tgcggcaagg agcacgccta catgccgatc cacgtcaagg
     4741 tgctgccgca gcacgaatac accgcctggg tcgagcagga acgcaagcgc ctggcggccc
     4801 tggccgacga cccgaacaag tcgtggacgc tggccgaact ggtggcgcgc ggcgaaaagg
     4861 tgtatgccgc caactgcgcc gcctgccacc tggaaaacgg ccgtggcagc gccgccatca
     4921 agccgctgga cggcagcgcc atcgtcaagg gtccggcggc cgagcagatg cgggtggtgc
     4981 tggaggggcg cgccaacggc accatgccgg cctggaagca gctgtccgac gtcgagctgg
     5041 cggcggtcgt cacctacacg cgcaaccact ggaccaacca gacgggcacg ctggtgcagc
     5101 cggccgaatt caaggcggcg cgcaccggca agttccccgc cggaggttga gcgtccgcgc
     5161 tgaaccctga cccgcccgtc caagtttcag aatcccggaa agattgccat gagcgccgta
     5221 ctgcccaccc cccacgccgg tcacgacgac cacgcccacg accaccatca cgcgcccacc
     5281 ggctggcgcc gctgggtgta cgccaccaac cacaaggaca tcggcacgct gtacatgctg
     5341 ttttccttct tcatgctgat ggtcggtggg gtgctggcgc tgctgatccg cgccgagctg
     5401 ttccagcccg gcctgcaggt ggtcaacccc gagctgttca accagctcac caccatgcat
     5461 ggtctgatca tggtgttcgg cgccatcatg ccggccttcg tcggcttcgc caactggatg
     5521 atcccgctgc agatcggcgc cagcgacatg gccttcgcgc gcatgaacaa cttcagcttc
     5581 tggctgctga ttccggccgc catcatgctg gtcggctcgt tcttcatgcc gggcggtgcg
     5641 ccggccgccg gctggacgct gtacgcgccg ctgacgctgc agatgggcat gtcgatggac
     5701 agcagcatcc tggccatgca catcatgggc gccagctcga tcatgggcgc catcaacatc
     5761 atcgtcacca tcctgaacat gcgcgcgccc ggcctgacgc tgatgaagat gcccatgttc
     5821 tgctggacct ggctgatcac cgcctacctg ctgatcgcgg tgatgccggt gctggccggc
     5881 gccatcacca tgacgctgac cgaccgccac ttcggcacca gcttcttcaa ccccgccggc
     5941 ggcggcgacc cggtgatgta ccagcacatc ttctggttct tcggtcaccc cgaggtctac
     6001 atcatgatcc tgccggcctt cggcatcatc agccatgtgg tgccggcctt cgcgcgcaag
     6061 aagctgttcg gctacgcctc catggtgtac gcggtggcgg ccatcgccat cctgtcgctg
     6121 atcgtgtggg cgcaccacat gttcaccacc ggcatgccgg tcacgggcca gctgttcttc
     6181 atgtacggca ccatgctgat tgccattccc accggggtga agatcttcaa ctgggtggcc
     6241 accatgtggc gcggctcgat gaccttcgag acgcccatgc tgtgggccgt cggcttcatc
     6301 ttcgtgttca ccatcggcgg cttcaccggc gtgatcccgg cggtggtgcc ggtggacaca
     6361 cagatccagg acacctacta catcatcgcc cacttccact acgtgctggt ggccggttcg
     6421 ctgttcgcgc tgtacggcgg cttctactac tggtcgccca agtggaccgg ggtgatgtac
     6481 aacgagacgc gcggcaagat ccatttctgg tggtcgatga tctccttcaa cgtcaccttc
     6541 tttccgatgc actttctggg tctggccggc atgccgcgcc gctacgccga ctacccgatg
     6601 cagttcgccg acttcaacct gatcgcctcg gtgggcgcct tcttcttcgg cttcgcgcag
     6661 ctgtatttct tcctgttcgt ggtgctgccg gtgatgcggg ggcagggcgc caaggcgccg
     6721 cagcgcccct gggaaggcgc cgagggtctg gaatgggaag tgccctcgcc ggcgccgttc
     6781 catacctacg aaaccccgcc caagctggac gccagcgcca cccgcgtgat cggctgatgg
     6841 tggacgaatc gcggatgtcg gccatgacca cggagcagca gcgcaagcgc aacgtccgtc
     6901 tggggctgat cctgggcacg gtggcgctgg cgttcttcgt cggcttcatc gtgcgcatgg
     6961 tggtgctggg cggctgacgg gcggggatgg ccatgcgccg gcgttccgaa aacctgaaga
     7021 tgctgggcaa gctggccgtg gtggcctgcg gcatgttcgc cttcggctac agcctgatcc
     7081 cggtctaccg cgccatctgc gaagccaccg gcatcaacat cctgtcgctg tccgagcgcc
     7141 aggtgccggg caacgggtcg gccggcgaga cggcgcggct ggccagtcgc aacacccagg
     7201 tcgatcgcag ccgcgtcatc acggtcgagt tcgacgccaa cacccgcggc ccttgggaat
     7261 tccggcccgc gcagcgcacc ctgcaggtgc acccgggcga gctggccacc gtgatgtacg
     7321 agttccagaa cgtgcaggat cggcgcatgg cggcgcaggc catccccagc tacgcgccgc
     7381 gccaggcggc gccgcatttc aacaagctgg aatgcttctg cttcaatcag tacacgctgg
     7441 agccgggcga gaagaaggaa tggccggtgg ccttcgtgat cgatccgcgc ctgcccaagg
     7501 acgtgaccac catcacgctc tcctacacct ttttcgaagt gggtggcaag acgcccgcgc
     7561 cgccgcagtc cagcgcggcg ctggcccgcc ctcacggagc cggcgcatga gcgagccggc
     7621 ccgcaccgtg cagaccccgg tgtcgccggc gccgcccgac gtggccgcgc cagccgcggc
     7681 gggtggcccg gccttctggc gcacgctgcg catggtgggc tggggctttc tgggcgtgcg
     7741 caagcgcagc gaataccagc gcgacctggc ccaggtcaat ccgtttcaca tcatgctggc
     7801 gggcctgatc ggcgtcgtgc tgttcgtgct gctgctgctg ggcatcatcc actgggtggt
     7861 gtccggcaca acccccggct gaggcccatc gataaccatc caatcaacgt ccagcgcagg
     7921 tgagacaagc gccagcagct ataaaattca gagcacagga gtgaagtgaa gatgtccagc
     7981 accacgcacg gtcagacgcc gtactactac gtccccgccg agtcgcggca tccggtgatg
     8041 gccgccttcg gcctgctgct catgatcatc ggtgccagcc agtggatcaa cgacgccgcc
     8101 tggggcggct ggctgctgct ggccggtctg ctgtggtggc tgttcgtgct gttccagtgg
     8161 ttcagcgatg ccgtgggcga gagcgaaggc gggctgtacg gtcgcaagat cgacctgtcc
     8221 taccgctgga gcatgagctg gttcatcttc tcggaggtga tgttcttcgg cgccttcttc
     8281 accgccctgt ggtgggcgcg ctcgcactcg gtgccggcgc tgggcagtct ggacaacgcg
     8341 ctgctgtggc ccgacttcaa ggccgtctgg cccagcgtgg cgcccggcgc caccgcctcg
     8401 ccggccggca ccgtgcaacc gttccagacc atggggccgt tctggctgcc gaccatcaac
     8461 accgcgctgc tgctgacctc gggcgtgacg ctgaccatcg cccaccatgc gctgctggcc
     8521 ggccagcggg cgcgcacggt ggcgttcatg tgggtcaccg tgctgctggg cgcgatcttc
     8581 ctgtgcgtgc agggctacga gtactaccac gcctacaccg acctcaacct caagctcagc
     8641 tccggcgtct atggctccac cttcttcatg ctgaccggct ttcacggcct gcacgtgctg
     8701 gtcggcatgc tgatgctgct gttcatcacc ctgcgcctga tgaagggcca tttcacgccc
     8761 cagcgccact tcggcttcga gggcgcggcc tggtactggc acttcgtgga cgtggtgtgg
     8821 ctgggcctgt acctgctggt gtactggatg taacaccgcg tcgcgttcag cagcgcgacc
     8881 ctgccgccgg ctgtgcccgg catcgcgatg accgcctcgg tcaggcgggc gcagcaaggt
     8941 cgttcgtggt ggtcttgaac gcccggtcgc cacagacttc gcccgcgcca cccccgaaga
     9001 gcgccctgcg gcgctctttt tgctgcggcg gccgcacgct cagaatgatg atgaaatgcc
     9061 gctgggctgg atgtagccca gtttccaggc cagcagaatg cacaggaaca gcagcaccga
     9121 caaaccgacg cgcagcgcca gcgagcgcat catgcggccg gcgcgccggc tgtccgtggc
     9181 ttcgccctgc gggtcgcgcc tgagcatgaa caccagcgcc gacgccaggc tggccagaat
     9241 gccgacgaac gccagcgcca cgaacacttg tttcatgggg cggattatcc ggtgagccgc
     9301 tggcgttcgc tgctggtgct gctggctgcg ctgctgggcg tggggctgac gtttgcgctc
     9361 gggcgctggc agctcgcgcg cgccgccgaa aagcaggcct tgcacgccgc catcgaggcg
     9421 cgccagtcgc tgcctgcgct cgatggcctg acgctggccg ggccactggc gcccgatcag
     9481 gcgcaggcgc tgctgcaccg ccgcatcacc ctgcgcggac gctggctgcc gcagcgcacc
     9541 gtctacctgg acaaccggcc catggggcgc cagaccggct tttacgtgct gacgccgctg
     9601 cggctgagtg gcagcgatgc ggtcgtgctg gtgcagcgcg gctgggcgcc gcgccaccgc
     9661 agcgagcgcg agacgctgcc gccggtgcag acgccggacg gcgaggtgca ggtgcatggt
     9721 cgcgtggccg aacatccgcc gaggctttat caactggggc aggaggggct cggggcgatc
     9781 cggcagaatc tggacctcga cgcctaccgt gccgaaaccg gcctgccgct ggccggcctg
     9841 atggtgtggc agactggcgc gccatccgag ggcctgcgcc gcgactggcc cgaggccgcc
     9901 agtggtgtcg acaagcacta cggctatgcg ttccagtggt tcggcctgtg cgccctcatc
     9961 ggcctgcttt ttgtgtggtt tcaagttgtc agacctatct accgtacccg acgcgcctga
    10021 gagcggccgg gccaaccagg cgctcggcat gaccgtgcac aacctgcctt cgcccgaagc
    10081 tctgctggcg cagcaggcgc ggcgcacgcg cgccggtcgc tggcagatgc tgctgctggc
    10141 gctggtgtgc gcggcgccgg tgatcgcctc ctactacagc tactacgtgg tgcggcccga
    10201 ggcgcggcgc agttttggcg agctgatcga gccgcagcgg ccgctgccgg tcgccagcgc
    10261 caccgacctg agcgggcgcc cggcggcact gtctgccctc aagggccagt ggttgctgat
    10321 cagcgtgggc ggcggcgcgt gcgatgcggt gtgtgaaaac cacctgtacc tgcagcgcca
    10381 gctgcgcgaa ggcctgggca aggacaagga ccggctggac tgggtctggc tggtcagcga
    10441 cgacgcgcgc ctgcccgagc cgctgttgcc ggcgctgggc caggccacgg tgctgcgtgt
    10501 gccgcccgac gtgctgggcg gctggctggc gccggcgccg gggcgggcgc tgcccgacca
    10561 cctgtacgtg gtcgacccca tgggccactg gatgatgcgt ttccccgccg ggctggacaa
    10621 gcagagcgcc ggccgcgcca aacgcgatct ggagcggctg atgcgcgcct cctccagctg
    10681 ggatcgggcg ggccgatgat ggacgccacg ccgctgtaca acctcagccc cgcgttgcgc
    10741 ctgatgggca tgggcctggt ggtggcgctg ggcccgctgg cctggctgtg gctgcgccac
    10801 cagggcgctg gcccggcgcg gcgcctgcag gcgctgaccc tgctgacgct gtttctgacc
    10861 ttcgacctgg tgatgttcgg cgccttcacg cgcctgaccg attccggtct gggctgcccc
    10921 gactggcccg gctgctacgg tcaggccagc ccggtcggcg cgcgtgtgca gatcgacgcc
    10981 gccgaggccg ccatgccgac cggcccggtg acgcacggca aggcctgggt cgagatgatc
    11041 caccgctacc tggccaccgg ggtgggcgtg ctgatcctgg tgctgaccct ggccacctgg
    11101 cgccagtggc gacgccagcg cgccgccgcc gcgaccgaca tcgtgcaccc gggctggccg
    11161 ctgctgacgt tgctgtgggt ctgcgcccag ggtgcctttg gcgcgctgac ggtcaccatg
    11221 aagctgtttc cggccatcgt caccctgcac ctgctgggcg gcgtgggcct gctggtgctg
    11281 ctggtggtgc aggccgaaag ctaccgccgc agcgccggtg ccgcgcccga gccgctggct
    11341 gccggcctgc gtgcgctgct gtggctgtcg gccggtgcgg tggcgctgca gatcgcgctg
    11401 ggcggctggg tcagcaccaa ctacgccgtg ctggtgtgca acacctttcc catgtgccag
    11461 ggcagctggt ggccgccgat ggactttgcc cagggcttcg agctgtggcg gccgctgggc
    11521 gagtcggcgg acggctcgcg gctggagttc catgcgctca ccgccatcca ctacacgcac
    11581 cggctgttcg cctacgccgt gttcctgctg ctgggcctgc tggcctggcg gctggcgcgc
    11641 gccggccggc tggcggcgca gcggcgctgg gtgctggggc tggcggcgct gcagttgctg
    11701 accggcctgt ccaacgtgat tctggactgg ccgctgctgg ccgccgtgct gcacaccggc
    11761 ggcgccgcgg cgctgatgac ggtgctgacc tgggccctgt gcgccagcca ccggccggag
    11821 ctgtcccgat gagcgccccc ggtaccgccg ccgtgctgcc gtccatgccg atctggaagc
    11881 agtactacgt gctgaccaag ccgcgcgtgg tgcagctgat cgtgttctgc gccctgatcg
    11941 gcatggtgct ggcggtgccg ggcctgccgg acgcagcggc gctgcagcgc atgctgatcg
    12001 cctgcgtcgg catctggctg gtggcggcgg cggcggcggc cttcaactgc ctggtcgaaa
    12061 aggccatcga cgcgcgcatg cggcgcacca gctggcgccc caccgcgcgc ggcgagctgt
    12121 cggccgcgca ggcgctgggg ttttcggcac tgctgtgcgc gctcggctcg gccgtgctct
    12181 acctgtgggt caacccgctg accatgtggc tgacgtttgc caccttcgtc ggctacgcca
    12241 tcatctacac cgtcatcctc aagccgctga cgccgcagaa catcgtcatc ggcggtgcct
    12301 cgggcgccat gccgccggtg ctgggctggg cggcgatggc gggcacggtg gagccgcagg
    12361 cgctgatcct gtttctgatc atctttctgt ggacgccgcc gcatttctgg gcgctggcgc
    12421 tgtaccgggt ggaggactac cgcaagagcg gcctgccgat gctgccggtg acgcacggca
    12481 acgagttcac gcgcctgcag gtgctgctct acaccctcat cctgctggcg gcctgcctgc
    12541 tgcccttcat ctaccgcatg agcggctggc tgtatctggt ggcggcgctg gtgttgaacg
    12601 ccggcttcat cggctacgcc ttccagctct ggcgccgcta ctccgacgcg ctggcgcgca
    12661 agaccttccg cttctcgctg atccacctgt cgctgctgtt tgcggcgctg ctggtcgatc
    12721 actacctgcc ctggacccgt tgaacatgga tcgtcgtctt gctattaaaa atctagctgc
    12781 tggcgctttg ctggtaagcg cctgggcctt gttgaccgct tgccagccgg acaggccgaa
    12841 ctttcacgga atcgacatca ccggggtcga ctacgcccgg gacctgcgcg tgcgcgactt
    12901 caatggccag ccgcgcacgc tggccgactt ccgcggcaag gtggtggtga tcttcttcgg
    12961 ctacacccag tgtcccgacg tctgccccac caccatgggc gagatgctgc gcgtcaagca
    13021 actgctgggc gccgatggcg acaagctgca ggtgctgttc ctcagcctgg accccgagcg
    13081 cgacacgccc gaggtgctca aggcctacat gggcagcttc gaccccggct tcctgggcct
    13141 gtacgccggc tccgccgacg agctggcggc gctggccaag gacttcaagg tgttctacaa
    13201 gaaggtcgag ggcagcacgc cgggcagcta caccatggac cacacggcct ccagctacat
    13261 ctacgacccg cagggccggc tgcgcctggt cagccgctac ggtacccggc ccgaggacgt
    13321 ggcggccgac gtgcgccagc tgctcggcgg cgcctgacgg cctgcccgca gggcggtcaa
    13381 aaccagcgcc agacctttca gggcgtgcgc caggcgctat cgtttcatga tcgtggcagg
    13441 gtgaccctca gtcgacccga atgccctgcg tgcggatgat ttggcccagc acgcgggtgt
    13501 cggcctggat gcggttggcc agcccttcgg gcgacgagcc caccgcctgc cagccctgct
    13561 gaaacaggcg ctggcgcatt tccggtgcgc gcacgatggt gctgatggcc tcggccagcc
    13621 gggccacatg ggcgcgcggc atgccggccg gcgccaccac ggcgttccag atttccaggt
    13681 tgaactgctt cacgccggcg tccagcaggc tgggcagttc gggcgccagc gggctgcggc
    13741 cgctgccggt gaccccgatg ccgcgcagct tgccggcctt gatctgcgtc tgcgccagcg
    13801 ccggtggcag catggacagt tgcaggtcgc cgccgaccag ggcgttgaac acctgcgggt
    13861 agccggggta gggtacatgc accggggcga tgccggtgcg cgccttcagc agctccatgc
    13921 ccaggtggcc gacggtgccg accccgggcg agccgtagct ccacttcgag ccggcggcgc
    13981 gcgcggcgtc caggaagctg cgggcgtcgc tgccgggcgc gctggccggc gccaccagca
    14041 ccaggggcgc cacgccgatc aggctgatgg gggcgaagtc ggtcgccggg tcgtagcgca
    14101 gggccgggtt gagcagcctg gcgatggtca ggttgccatt gatcatcagg ccaatggtgt
    14161 gaccgtcggt ggccttggcc acggcgtcgc cgccgatgtt gccgccggcg ccgacacggt
    14221 tttccaccac caccggctgg cccagggctt tttccagcgg ctcggccagt gcgcgcgccg
    14281 tcaggtcggg cgaggagccg gccggaaagc cgacgatgaa gcgcagcggt ttgctgggcc
    14341 aggctgcggc agtctgtgcg tggctggtgg tcaggggggc gagcaggccg gcgcagacgg
    14401 cggcgcccgc gagcagacgg cggcgggtga acgtggggtg catggtcggg cggtgcaggg
    14461 caagggtcag agagggaagg gcgccgggga accggcaacg gcaacctgaa aacgactgtt
    14521 ggacgtgccc gcccgcgccg cgctcggcgt gccaggcagg gtgcgacgcc gcgagaccgg
    14581 ttcggatgcg cctctcgcca agctcacccg cgggccacgg ccgttgtcag attcaaagac
    14641 tatagctctt cgcgcagggc cggcgtgccc gagcccccgg gcggcatgaa aaaccccgcc
    14701 gacggggccg tgggcggggt gcaggggcag gccgcgcagg cgcgcggctc aggcgtagtc
    14761 ggccagtgcg gtgcgcattt tcttcatggc cgccacctcg atctggcgga tgcgctcggc
    14821 gctgacgccg tattcggccg ccagctcatg cagcgtcatg ccgcccgagc cgtcgtcgtt
    14881 gacgttcagc cagcgctcgg tgacgatgcg gcggctgcgc tcgtccaggc tgcgcaaggc
    14941 ggtggcgatg ccttcggtgg ccagcacgtc gcgctgggcc gattcgagca tggcggtcgg
    15001 ctcgtggcgc tcgtccgaca ggtaggcgat ggggccgaag gcctgttcgc cgtcgtcggc
    15061 cggcgccgga tccagcagca cgtcgccgcc ggccaggcgg gtttccattt cgcgcacctc
    15121 gtcgggcttg acgttcagct cgcgcgccac gacgtcgatc tgctcctcgg tcagggcgtc
    15181 gcggtgcgcc agatcggcgt cgagttcggc cttgaaaccc tgcttcatgg agcgcaggtt
    15241 gaagaacagc ttgcgctgcg ccttggtggt ggcgaccttc accaggcgcc agttcttcag
    15301 gatgtattcg tggatctcgg ccttgatcca gtgcagcgcg tagctcacca ggcgcacgcc
    15361 ctggtcgggg tcgaagcgct tgacggcctt catcaggccg acgttgcctt cctggatcag
    15421 gtcggcgtgc ggcaggccat agcccaggta ctggcgcgag gtggccacca ccagccgcag
    15481 gtgcgacagc accagccgac cggcggcttc caggctgccg gtgtcgcgca gctcgcgggc
    15541 gtagcgctgc tcttcctcca gcgtcagcat cggcaggcga ttgaccgcgc tgatgtaggc
    15601 gtccagattg cccaacggag gcaccaccga ccaggggttg gtcagtgcga cggcgttgct
    15661 gctggggctg gataccttct gggtcatcgt tgaatgtcct ttcgtgtggc tcaagtattt
    15721 tagcactcga ttggagggag tgctaagggc aaagttccgc ccgcgcccga accatggctg
    15781 accgggccgg cgggcgcgcc gggccgggcg cgcggctgcg cccgctacac tcgcgcgcat
    15841 cgcctgcgcc ttacaaaaga ggaccccgac catgcccttg cgatctgtcg gcctggtggc
    15901 cgcgctgctg ctggccggcc acgcacaggc cgcccacggc taccagggcc gcgtgcacgg
    15961 cagctttgcc gggcatgcca tcgatgtgcc ggtgatctgc gagcgcccca ggctggcggg
    16021 gcagcggggc aactggttct acgcgcagtc cgacccgccg acgcacgaat acgcccagga
    16081 ccgcaacggc gacggcgtgg cggtgacggt ctcgttcagc ggtcaggagg ccatgttcat
    16141 gctctacctg gcaggccagg accacaactt cggcaacacc cggcgtgagc agatcgtgct
    16201 caccgaaggc ggcttcgtgc tcaccatgag cgcctcgcgc tacgaaggca agggccggct
    16261 gcgccgaaag gtaggcgaag acgagatccg cctggccgtc gactgccctc agcgctgagc
    16321 cccgccggat cgccggcgtt ccagatcaaa ccgggattaa aggcgcacca atactgcgca
    16381 agcagctata gttttggtag tatttcagaa gctgcccaga tgcgtcagcg cctgggcgcg
    16441 cagcagcgtc agcgccgcct ggcgggtggc gctggccggg cgcagggcgc tgagggcggt
    16501 gtagagcggg atctgcagcg gctggccgtc cggcgtcgcg atgcggcgca gcgcaaaggc
    16561 ctcggggtgc gcggtgccgg ccagggcgtg gcgcgacagg atggcgccgc cggcgccgcc
    16621 ttcgaccagc gccaggatgg tggccacgcc atcgatctcc agcgccacct gcggcgtgca
    16681 gccggccgcg gccatctgcg tctcgacgtg catgcggatg gcgttgggcc ggctggggat
    16741 gaccaggggc agctgcgcaa ggtcgcgcag cgtggcagga gcggcgtcgg cgcgggcgcc
    16801 ggcgtgctgc accagcagca gctcgtcgcg cgccagcggc gcgatgtcca gcccgggcac
    16861 cggctgggcg ttatagagca cggcgatgtc cagccgcccg ctggccagtt gctcctgcat
    16921 gctggccgac agcgcctcgg tgatcgacag ctgggcttcg ggcaactgcc cgccgaaggc
    16981 ctgcaccagc ggtaccgcca gggcgcgcgc cacgctgggc ggcaggccca gggtcacgcg
    17041 accggccagg ccgccgcgca ggcgcgccag ctcgtcgcgc gcgtgttcga tctggtgcag
    17101 gatgccgcgc gcgtgctcca gcagcacctg gccggcttcg gtgggcatgg cgccgcggcc
    17161 gtggcgtgtc agcaggttct gacgcagctc cacttccagc aggcgcacct ggcggctgag
    17221 cgcgggctgg gccacgtcca gcgccagggc ggcgcggctg aagctgccca gctcggccac
    17281 gcggacgaag tattcgagtt gacgcaggtc catggtccca atttacgaca tggctgtgat
    17341 tcaggtatgc cattttgctc tagctgctag tggggtgggt ggctggtcag cggccctgcc
    17401 gctgcgcaca atgcgccagc gtcagcagag gaacaccatg agcttgcacc accccctggt
    17461 catcgactgc cacggccact acaccaccgc gcccaaggcg ctcgaacagt ggcgcaaccg
    17521 gcagatcgcc ggcattcagg acccggcagc gatgccgcgc gtcgacgagc tgcggatcag
    17581 cgacgacgaa ctgcgcgaat cgatcgagaa caaccagctg cgcctgatgc gcgagcgcgg
    17641 cagcgacctg accattttca gcccgcgcgc cagcttcatg gcgcatcaca tcggcgactt
    17701 tcaggtctcc agcacctggg cggcgatctg caatgagttg tgccaccgcg tcagccagct
    17761 gtttcccgag catttcattg gtgccgccat gctgccgcag tcgcccgggg tcgatccggc
    17821 cagctgcatt cccgagctgg agcggtgcgt gcgcgactac ggcttcgtgg gcatcaacct
    17881 gaaccccgac ccttccggcg gtcactggac cagcccgccg ctgaccgacc gccactggta
    17941 cccgatctac gaaaaaatgg tcgagtggga catcccggcc atgatccacg tcagcacgtc
    18001 ctgcaacgcg tgctttcaca ccaccggggc gcactacatc aacgccgaca ccaccgccgt
    18061 gatgcagctc attcagggcg atctgttcaa ggacttcccg acgctgcgct tcatcatccc
    18121 gcacggtggc ggcgccgcgc cctaccactg ggggcgctac cgcggcctgg cgcaggagct
    18181 gaaaaagccc ctgctgcagg agcacctgct gaacaacctg ttcttcgaca cctgcgtcta
    18241 tcaccagccg ggcatcgacc tgctgaccaa ggtgattccg gtcgacaaca tcctgttcgc
    18301 cagcgagatg atcggtgccg tgcgcggcat cgatcctgaa accgggcact acttcgacga
    18361 cacccggcgc tacgtgcagg ccaccgccaa cctgaacgac gagcagcgct acaaggtcta
    18421 cgaaggcaac gcgcggcgcg tgtttccgcg gctggacgcg gcactcaagg ccagggggag
    18481 atgagccatg tatgaactcg gagtggtgta tcgcaacatc ccgcgcgccg acggcgccac
    18541 ggccgacgcg ctggcgcagt tcggcagcgc caccgtgcac gaggccatgg gccgcgtcgg
    18601 cctgctcaag ccctacatgc ggcccatcta ccccggcgcg caggtcagcg gcacggccgt
    18661 cacggtgctg ctgcagccgg gcgacaactg gatgctgcac gtggcggccg agcaaatccg
    18721 cccgggcgac atcgtggtgg cggcggtcac ggccgaatgc accgacggct acttcggcga
    18781 cctgctggcc accagcttcc aggcccgcgg cgcgcgcgcc ctcatcatcg acgccggcgt
    18841 gcgcgacgtg cgcaccctgc agcagatggg ctttccggtc tggagcaagg cgatcagcgc
    18901 caagggctgc atcaaggcca cgctgggctc ggtcaacatc cccgtcgtgt gcgccggcat
    18961 gctggtgacg ccgggcgacg tgatcgtggc cgacgacgac ggtgtggtct gcgtgcccgc
    19021 cgcgcgcgcg gtgcagacgc tggaagcggc gcgcaagcgc gaggccaacg aaggcgacaa
    19081 gcgcgagatc ttcgccagcg gcacgctggg gctggactat tacaagatgc gcgaagggct
    19141 ggccaaggcc gggctgcgct acatcgattg agggccgcgc gatgagcaaa cccaccagcg
    19201 gcgacttcac caagacggcc ggttggctcg attggtacga cggccccagc accccgcgtt
    19261 tcaggctgcc ggccggcagc gtggacgcgc actgccacgt gttcggcccg ggcgccgaat
    19321 ttccctacgc gcccgagcgc aagtacacgc cctgcgacgc cggcaaggcc cagttgttcg
    19381 cgctgcgcga ccatctcggc tttgcgcgca acgtgatcgt gcaggccacc tgccacggcg
    19441 ccgacaaccg ggcgctggtg gatgcgctgc aagcctccgg cggccgggcg cgcggcgtgg
    19501 ccacggtgcg ccgcagcgtc accgacgacg aactgcaggc cctgcacgcg gccggcgtgc
    19561 gcggcgtgcg cttcaacttc gtcaagcgcc tggtcgactt cacgcccaag gacgaactga
    19621 tggagatcgc cggccgcatc gcccggctgg gctggcacgt ggtgatctac ttcgaggcgg
    19681 tcgatctgcc cgagctgtgg gacttcttca ccgccctgcc caccaccgtg gtggtcgatc
    19741 acatgggccg ccccgacgtc ggcaaaccgg tcgacggccc cgagttcgcg ctgttttgca
    19801 agttcatgcg cgagcacgcc aacgtgtgga gcaaggtgag ctgccccgaa cgcctgtcgg
    19861 tcagcggccc gccggcgctc agcggcgagc aggccgccta ccgcgacgtg gtgccgttcg
    19921 cccgccacat cgtcgaaagc tttccggacc gcgtgctgtg gggcaccgac tggccacacc
    19981 ccaacctcaa gaaccacatg cccgacgatg ggctgctggt ggacttcatc ccgcacatcg
    20041 cgcccacgcc cgagctgcaa cgcaagctgc tggtggacaa cccgatgcgc ctgtactggc
    20101 ccgaggcatg atgattcccc ctgagtcgct tcgcgccttc ccccggaggg ggacaacgcc
    20161 agtggccggc gcaggtccgg ccacggcgtt cgctggtatg gcctgctcct cggcctccgg
    20221 cttcattggt gatcgatcgc gctgactgcg caggagtaat ggcgatgtct ctcaacaaac
    20281 cctatctcga cgttcccggc accaccatct tcgatgccga ccagagccgc aagggctact
    20341 ggctcaacca gttctgcatg agcctgatga aggccgacaa ccgcgcccgc ttcaagcagg
    20401 acgaggacgc ctacctggcc gagtggccca tgaccgacga gcagaaggcg gcggtgcgcg
    20461 cgcgcgacct gaactggatg atggcgaccg gcggcaacat ctacttcctg tccaagctgg
    20521 gcgccaccga cggcctgtcg tttcagcaga tggcgggcag catgaccggc atgaccgagc
    20581 agcagtaccg cgacatgatg gtgcgcggcg gccgccaccc cgagggcaac cgctacaagg
    20641 gcgagggcgg cgacgcccag ggcccgatga gccagtccga agcctggaag gcctggcagg
    20701 ccaaggcaca aaatgggctg gcagcgtccg tccagcaagc gcagacagct gccaattcaa
    20761 tagcaaccat gggcgcccgc atcaccgcct cggtgtattt ctcgcatgtg ccggcgatcg
    20821 gcgccgccat cgaccacggc aagacggccg agccctactg ggcgcccatg ttccagggtt
    20881 tcgagttcag caagcggtgg atgaaggagc acctgcccga cgtcatcttt ctcgtctaca
    20941 acgaccacgc caccaacttc gacctcagcg tcatccccac cttcgccatc ggcaccgcgg
    21001 ccgaattcga gcccgccgac gaaggctacg gcccgcgccc ggtacccagg gtcaagggcc
    21061 acccgcgcct ggcctcgcac atcgcgcagt cggtgatcca gcaggacttc gacctcacca
    21121 tcgtcaacca gctcaaggtc gatcacggcc tgacggtgcc gatgaacctg atgttcggca
    21181 cccccgccga atggccctgc gcggtgattc cgttccacgt caacgtggtg cagtacccgg
    21241 tgccctcggg cgcgcgctgc tttgcgctgg gccaagcgat ccgccgcgcc atcgaaagct
    21301 acgacgagcc gctgaaggtg cagatctggg gcaccggcgg catgagccac cagttgcagg
    21361 gcccgcgcgc cggcctgatc aacgccgagt gggacaaccg cttcctggac cgcctgatcg
    21421 ccgacccgaa gggcctgtcc gccatgccgc acatcgacta cgtgcgcgaa gccggcagcg
    21481 agggcatcga gctggtgatg tggctgatcg cgcgcggcgc catggccgac gtggcgggcg
    21541 gccccgcgcc caaggtggcg caccgcttct tccatgtgcc ggcgtccaac acggcggtgg
    21601 gccacctgat cctggaaaat caagcttgaa tcggcttgcg gcgcttgtct ggcaagcgcc
    21661 agaagctatt gttataggag catcatgagc aagaccatca acgttgcgct ggccggcgcc
    21721 ggcgccttcg gcatcaagca cctggacggc atccgcaaca tcgacggcgt gcgggtcgtc
    21781 tcgctggtcg gtcgcgagct ggacaagacg aaggaggtgg ctgccaagta cggcatttcc
    21841 cacgtgacca ccgacctggc cgacagcctg gccctgccgg agctcgacgc cgtcatcctg
    21901 tgcaccccca cccagatgca cgccgcccag gccatgcagt gcctgcaggc cggcaagcat
    21961 gtgcaggtcg agatcccgct gtgcgacaag ctggccgatg gccaacaggt ggcggcgctg
    22021 cagaagcaga ccggtctggt cgccatgtgc ggccacaccc ggcgcttcaa ccccagccac
    22081 cagtacgttc accagaagat cgcggcgggc gaattccaca tccagcagat ggacgtgcag
    22141 acctacttct ttcgccgcac caacatgaac gccctgggcc agccgcgctc gtggaccgac
    22201 cacctgctgt ggcaccacgc ggcgcacacg gtcgatctgt tcgcgtatca ggccggcagc
    22261 ccgatcgtgc aggccaacgc catccagggc ccgatccaca aggagctggg catcgccatg
    22321 gacatgagta tccagctcaa ggcggccaat ggcgcgatct gcacgctgtc cttgagcttc
    22381 aacaacgacg gcccgctggg caccttcttt cgctacattg gcgacagcgc cacctacatc
    22441 gcgcgctacg acgacctggt caatggcaag gaagaaaaga tcgacgtgtc ccaggtggcg
    22501 gtgtcgatga acggcatcga gctgcaggac cgcgagttct tcgccgccat ccgcg
//
LOCUS       DAOQTZ010000039        21979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2786_length_21979_cov_14.040275, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000039 DAOQTZ010000000
VERSION     DAOQTZ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 21979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21979
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2786_length_21979_cov_14.040275"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKN51_06505"
     CDS             complement(<1..562)
                     /locus_tag="PKN51_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNT84786.1"
                     /translation="MIDEARFAQRACAPAPARRASPATWIGRCLMLSLALLTGCSTVR
                     PWINTPMAPEQLRAGHLNETRDPTLVMAVTISGGGARAAAFGYGVLRALDEVRFDWNG
                     KHLTLLDATDLISGVSGGSIVSAYYAAFGREGLSNFEPEFLRKNFQQGLISLLTHPGS
                     LHELSSPWFGRSNLLQRQLDVLYRGMT"
     gene            803..2713
                     /locus_tag="PKN51_06510"
     CDS             803..2713
                     /locus_tag="PKN51_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNT84787.1"
                     /translation="MTRPFVDFARRRGWLAAALLACLLALPGCALLPSTDPARADASG
                     ERPAPAPQRSDTARDAPTASAFDLRVECDDAELRTLVERHAELLRYRAITDLDEAELA
                     RLLVLAERDVRQLLATEGYFSPVVKLRRDGTRGARPTVVIAIEPGAPTTVARVDIGFE
                     GQIASDDDADAVAQRRAIVDGWSLDQGRRFTQQRWSDAKTDALRALVARRYPRGRIAY
                     SLADVDAAGGRAHLHLRLDSGPLFRLGPAQVQGAQRYPPELAERLSWLRPGQVYDQKQ
                     LVDAQQRLTGSGYYDSAYLSIDPEGDAAAVPVSFTVTEARRHKVQLGVGYSTDAGPRL
                     TLEHRDNRAFASSWRSQTRLNLNRKTPLLQAELTSLPDAGGWRWGALARYQQQDDGLL
                     NTLSRTLRIGRSRIEATYDRNVYLNYEHASVTGSATRTVPDALLGDGAALSAGYAWTG
                     RHFDRLPLPTRGWGLGLDASAGLTTVGARRPFARLTAHWLGIVPLAGGASRLALRAEG
                     GAVIADRRARLPGSHLFRTGGDTTVRGYGWRRIGMPVGQGLVGPGRYLALGSVEWQRP
                     ILQNRWPGLLEHSLFIDVGSVANRADRLRPHWGVGTGLRMITPVGPMQLDIAYGLKSK
                     EIRLHMTVGLVF"
     assembly_gap    2850..2949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2950..6890
                     /locus_tag="PKN51_06515"
     CDS             <2950..6890
                     /locus_tag="PKN51_06515"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNT84788.1"
                     /translation="QPPARRRGLVRWPQRLLAGLLAAAGLAAGGLWLWAGSEGSLATA
                     LAWVGRHQPLQAEGVRGTLRGGGTLQRLVWSQDGLQLQAEQAEWRWHPGALLWRRLHI
                     ERLSAHRLRIDDQRPADRPTARPPEALTLPLQVRVAELRVDELHWRGPPALRLDDIAG
                     HYAYDGGQHRLTLERARFEQGRYRLHAAATAHAPIRLDLALTGALSATVPGGAAALPL
                     TLQATLRGPLTELQAQADLQAAAPGTAPEPPALPPLLGFAARAPQQQPRAPTPPAAAA
                     QASPQARLSARLTPWAAQPVAEAHARVHALDLAALWSQAPHTRLNGALDLMPLPGTAQ
                     TGWALVADLTNPAAGPWDQGRLPVQALQADALWQDDTARVRTLKARAGGGTLELDGHW
                     RRATEAAPPWQIDARLSGVDPAQLHGQLAAFPIDGTARLRGAGHATDFELALQARTSP
                     APAAAPPARDTLAHGLRALRLQSAHAQGRWDAGLLRLERLQLRSDDAELSGHAQWRLQ
                     GSDAPGGRAELRLSAPGLRASAQGELHAQRGGGQLSAELSDAARALAWARRLPGSQRW
                     LGDAQAQGRATLQARWRGGWRDPTLDARLSVPKLDWRGAGSPPLQLRELQLTADGRLA
                     QARLDLRGQIAQGERRLRLALRASGGRSTPDTPLAQASWRVQVEPWQAEWHDPALGAG
                     AWQLAGQGVLPLRWSPARGGQFEAGAGTLTITSPAPASQARLSWEPLRWQAGELDSRG
                     RLSGLPQQWLDRLAGTPLQDAGLSGDLVFDGAWQATLGRQLRLDARLARARGDLTLQL
                     TDADTGVRSRVAAGLRQAQLSLRSDGPALTLDLLWDSERAGRVEGQLRTALVAGSDAD
                     GRTRWSWPPDAALAGSLRAQLPQIAAWSPLAPPGWRLGGALAAELQLAGTRAAPSVTG
                     SLSADGVALRSVVDGLQFKDGRLRARLDGARLRIDEFSLAGVGASGSGGRLIASGEAA
                     WRDGRPQAQLNATLQRLRVSARDDRQLSVSGALQAALDGRALRTTGRLTVDQALIVLP
                     DDSRPTLGDDVIVRGPGGQVRAGRQADGRAPPPSGAPDAPARTPLNAHVDVQIDLGPE
                     FRLRGMGIDTRLAGLLTLTADGPLTGLPHVRGTVRTVGGTFHAYSQQLRIERGQLLFQ
                     GAADNPALDILALRPQLGHDQRVGVQVAGTALLPRVRLYSAPALPDNQTLAWLLLGRP
                     APDTGAEAAMLQSAALALLGGREGRGLAARFGLDELSFSGTGDGELNQASVTLGKRLS
                     ERLYAAYEHSIAGTGGTLMIFYELSRRWSLRGQAGAENSALDLIFKLSFD"
     gene            complement(6921..7355)
                     /locus_tag="PKN51_06520"
     CDS             complement(6921..7355)
                     /locus_tag="PKN51_06520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT84789.1"
                     /translation="MKIELPEHKQLVFEMTIPIRWGDMDAMGHINNAVYFRYMETARI
                     DWLHGIGCQPDPGGEGVVIVNAFCNFIRQFTYPGDILIKTFVSHLGRSSFDTWATMER
                     TDQPGVVCATGGATVVWVNFPAQKSAPLPDWLRQRLSPAPGG"
     gene            complement(7449..8231)
                     /locus_tag="PKN51_06525"
     CDS             complement(7449..8231)
                     /locus_tag="PKN51_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT84790.1"
                     /translation="MSYSIDLSGRVALVTGASGGLGAQFARTLARAGAGVVLASRRLE
                     KLKDLRARIEGDGGDAHVVECDVTDVHSIRAAVAHAETEMGSIDILVNNSGVSTTQRI
                     QDVSEQDYDFVFDTNVRGAFFVAQEVGKRMLARARGAAPGSFTGGRIINIASMAGLRA
                     LPQIGVYCMSKAAVIQMTRAMALEWGRHGINVNAICPGYIDTEINHHHWQTEKGRKLI
                     DMLPRKRVGEPADLDAVLMMLASDQSHFVNGAVIAADDGFAL"
     gene            8393..10093
                     /locus_tag="PKN51_06530"
     CDS             8393..10093
                     /locus_tag="PKN51_06530"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNT84791.1"
                     /translation="MTPEQIIQQYGPREAMEYDVVIVGAGPSGLSAAIRIKQRAAEQG
                     QEVSVVVLEKGSEPGAHILSGAVMDPKALTELIPDWKAKGAPLNQPVTDDAYVFLSET
                     GGTRVPGVLLPPFAHNHGNYIVSLGNVVRWLAEQAEALGVEIFPGFAAAEVLFNDDGS
                     VKGVATGNMGVGKDGEPTENFQLGMELHAKYTIFAEGARGHLGKQLIARFKLDAQSDP
                     QSYAIGIKELWEIDPSRHTPGFVMHTAGWPMDEHTYGGAFLYHAEDHKVYCGYVVGLN
                     YRNPYLSPFEEFQRWKTHPRIKWYFTDDRGQVNAKRLSYGARAITAGGVLALPRTIFP
                     GGALVGCDAGYLNVSRIKGSHAAIKTGSLAGEAACDAVLAGRQHDELTAYPEAFDHSW
                     LAAELNKDRNFKNWFKKGLTVGTVMNGFEQYVLRGHAPWTLHRAEPDHVQLKAAAECA
                     PIDYPKPDGKLTFDRLSSVFISNTNHEENQPAHLTLKDPGVPVNVNLAKFAGPEGRYC
                     PAGVYEFVPDEARGGNAQRLQINAQNCVHCKTCDIKDPTQNIVWVTPEGGGGPNYSGM
                     "
     gene            complement(10140..10940)
                     /locus_tag="PKN51_06535"
     CDS             complement(10140..10940)
                     /locus_tag="PKN51_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HNT84792.1"
                     /translation="MPRFAANLSMLYNELDFLDRFAAAASDGFTAVEYLFPYAWAPEQ
                     LSALLRQHGLRQVLFNAPPGEWDAGERGLACIPGREGEFHAGLERAIAYARALDCPRI
                     HVMAGLMPAGVDAATLRATYVANLRGAAEQAAPHGIDILIEPINPRDMPGFYLQRQDQ
                     AHALLDAIDRPNVKVQMDLYHCQIVEGDLAMKIRHHLPGGRVGHIQIAGVPQRHEPDL
                     GEIHHPYLFELLDRLGYEGWIGCEYRPARGAVPGGTSDGLGWLRPYLA"
     gene            complement(10951..11613)
                     /locus_tag="PKN51_06540"
     CDS             complement(10951..11613)
                     /locus_tag="PKN51_06540"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HNT84793.1"
                     /translation="MSEARAREEIVRVGRSLFERGYVHATAGNISVRLADGYLITPTD
                     ACLGTLDPARLARLDAQGRQTGGDPASKTLALHRRIYEASAGTAAPAGCVVHSHSTHL
                     VACSLRADPAAAELLPPLTPYFVMKVGRVPHIPYHRPGAPEAAEAVARAIARHAEAGH
                     PIRAVMLARLGPNVWHDTPAAAMATLEELEETARLWLLAPAAPLNTAQIDELRRAFNA
                     FW"
     gene            complement(11625..12908)
                     /locus_tag="PKN51_06545"
     CDS             complement(11625..12908)
                     /locus_tag="PKN51_06545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HNT84794.1"
                     /translation="MTPPILLGCIADDFTGATDLANNLVRAGMRVVQTIGVPEAPLAA
                     DAAADAVVVALKSRTIAADQAVAQSLAALRWLRAQGARQIYFKYCSTFDSTPAGNIGP
                     VTEALMQALDCDFTIATPAFPDNGRTVFKGYLFVGDQLLSESGMRQHPLTPMTDANLV
                     RVLQAQTRRRVGLIEQRTLAAGSDAIRARIADLRAEGVGIAIVDAVSNDDLWRLGPAL
                     AELPLVTAGSGVAIALPANFGLAPAAQAAALPAASGLRAVLAGSCSQATQAQVAHFIA
                     CGHPALALDPLRMAAGDAVVRQALDWAQGRLAADAPLLVYSTAEPAAVQAIQQRLGVA
                     RAGELVEQTLAAIARGLVGLGVRQLVVAGGETSGACVQALGVAQLRIGAQIDPGVPWC
                     HAASPLAPQGLHIALKSGNFGGEDFFTRAFAVLRP"
     gene            complement(12905..13813)
                     /locus_tag="PKN51_06550"
     CDS             complement(12905..13813)
                     /locus_tag="PKN51_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNT84795.1"
                     /translation="MSDRHVGVIGLGAMGLGIACTLRQRGYRVRACDARAGVAAAFAA
                     EGGVACTTPAEVANGVRVVVSVVVNAAQTEQVLFGSDGAAAAMAPGSVFVMCSTVDPN
                     WSMALEARLAQHGILYVDGPISGGAARAASGQMTMMTAATPAAYAAADAVLEAMAGKV
                     YRLGQRAGAGSQVKIINQLLAGVHIAAAAEAMALGLREGVDPAALYEVITHSAGNSWM
                     FENRMAHVLAGDYTPLSAVDIFVKDLGLVLDTARASKFPLPLAATAHQMFMQASSAGH
                     ALEDDSAVIKIFPGIELPAPTDKERP"
     gene            13978..15006
                     /locus_tag="PKN51_06555"
     CDS             13978..15006
                     /locus_tag="PKN51_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT84796.1"
                     /translation="MHIVITGGAGFLGVRLARALLQRDALSLAGAAPRPLTRLTLVDR
                     VAPPAALTQDPRVRVVTGELDALLTPDASGQTRAVPTDSDAVVHLAAAVSGECEADFD
                     LGLRSNLDATRALLQTCRALGTRPLFVFSSSLAVFGHTPEQPMPAIIEDDTLPTPQNS
                     YGTQKYMSELLVADCTRKGFVRGRNVRLMTVSVRPGRPNAAASSFLSGIVREPLAGQR
                     AVCPVAPDTRVALSSPARTIEGLVRALEASDEDWGPRTALNLPALSTTAGDMVAALAR
                     VAGPEVAARVDWVADAGIARIVGAWPAGMNARRAARLGLRADASFDDIIRDHLRENPV
                     PPAAAARP"
     gene            15078..16049
                     /locus_tag="PKN51_06560"
     CDS             15078..16049
                     /locus_tag="PKN51_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT84797.1"
                     /translation="MKLHQLALGLGLALGLVAGAQAQTTMKISISVAQNSHQGIAIDT
                     FAKEVEKRTGGRYKIQTFYSGALGGERESIEAVQLGTQELAFSSTGPVPNFVPEVKIL
                     DVPFLFRDKAHARAVLDGPIGQDMLAKFEPKGFKALAWAENGFRHMTNSKRDVKTPED
                     LKGLKMRTMENPVHVAAYKGFGIITTPMAFPEVFTALQQGTVDGQENPLSVIISAKFD
                     QVQKHLTLTGHVYSPAIFLMNKGAFDKLSAADKQAFIEAAKEGTKANRARVDEDDAKG
                     VADLRAKGMTVIDTIDKAKFVSALAPVNAQFEKDFGKANLDRIRNFK"
     gene            16154..16648
                     /locus_tag="PKN51_06565"
     CDS             16154..16648
                     /locus_tag="PKN51_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNT84798.1"
                     /translation="MKAFILTLEGWTTRIAMVLACLMLALAAALGVFQIFTRFVLEQP
                     AEWSEVLIRFSLIWMVFLGIPMAFRQGAMVSVDALYRAVPTGAKRVLDLVVALAALAL
                     MLIVLWWGWDYAVRGSVQTMAGLESLSMFWAYLAMPVGAAFSLLGILANYLDPRRMEL
                     ETAQ"
     gene            16719..17993
                     /locus_tag="PKN51_06570"
     CDS             16719..17993
                     /locus_tag="PKN51_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNT84799.1"
                     /translation="MTSAMLITMVLGFALSVSVAVSIGLAAIVGIQASQAHMLISAKE
                     MFNAINKFPLAAIPFFILAGNLMETGGISRRLVEFAKSLVGGVQGGLPMTCVLTCMIF
                     AAVSGSSVATTFAIGAILIPALIKHGYPTTWAAALQATSAELGVIIPPSIPMILFGVS
                     AEVSIGELFIAGFGPGLLISGALMLFVWLYCKWQGWGRNDGDGRLGVVAATRQAAWAL
                     LMPVIILGGIYGGIFTPTEASAVAVLYALVVGMLIYREIGVKDLYVTLRKSVVSSAVI
                     MFIIANAGLFAFLITRAGVPEAIGHWLEQVLQNPALFLLGVNAALFVIGMFIETSAAI
                     IVLAPILVPVAQRFGIDPVHFGLVMVVNLALGMITPPFGVNLFAACTVARVSLDKIIR
                     HLVPFVGVVLACLMVITYVPTLSLALRDLVYR"
     regulatory      18019..18099
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      18100..18191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            18251..19381
                     /gene="gcvT"
                     /locus_tag="PKN51_06575"
     CDS             18251..19381
                     /gene="gcvT"
                     /locus_tag="PKN51_06575"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNT84800.1"
                     /translation="MSAADSPLLKTPLHALHLELGARMVPFAGYEMPVQYQAGLIAEH
                     RHTREAAGLFDVSHMGQLKLIGPDAAAALETLMPVDVQGLAENKQRYGLLLTDEGTII
                     DDLMFVNRGDHLFLIVNGACKAGDIAHMQARIGSRCLVQPLPDQALLALQGPQAVGAL
                     QRLVAGVEKLVFMTGGAFDWLGTPLYVTRSGYTGEDGFEISLPGERAEAFARALLAQP
                     EVKPIGLGARNSLRLEAGLCLYGNDIDHTTSPVEAGLNWAMQKVRRSGGARAGGFPGA
                     ARILAQLDGTQPVERQRIALMARERVPVREPAELRNLDGQQVGQVTSGLLSPTLDKPI
                     AMGYVRPGFATIGTELFAMVRGKPVPMEVVATPFVPNRYHRG"
     gene            19447..19815
                     /gene="gcvH"
                     /locus_tag="PKN51_06580"
     CDS             19447..19815
                     /gene="gcvH"
                     /locus_tag="PKN51_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNT84801.1"
                     /translation="MSLKYTEDHEWLKIEGDVATVGITHHAQDALGDVVFVELPEVGR
                     HYAQKDAAGVVESVKAAADVYMPVSGEVLEVNEALREDPSLANSDPQGAGWFFRIRLS
                     EPAQAGALMDEAAYTAFSAG"
     gene            19965..>21979
                     /gene="gcvP"
                     /locus_tag="PKN51_06585"
     CDS             19965..>21979
                     /gene="gcvP"
                     /locus_tag="PKN51_06585"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNT84802.1"
                     /translation="MPPMSQPTLPELENPGEFIARHIGPSPAEEAHMLSVIGEASRRA
                     LIDSILPPDIARRTPMDLPPACSEADALAELKAIASRNRHQMKSFIGQGYYGCHLPGV
                     ILRNILENPAWYTAYTPYQAEISQGRMEALVNFQTMVTDLTGMDIANASMLDEATAAA
                     EAMTLARRTVKAKGQVIVLSGDCHPQTIEVMRTRAAPLGLTLRLANSAAEWDAAIAAD
                     DYFAALIQYPASSGWLMDWSDEVEKIHAKGAAAIFAADLLALTLLKTPGEMGADIVVG
                     STQRFGMPMGAGGPHAAYMACRDDYKRSMPGRLVGVSVDAHGQPAYRLALQTREQHIR
                     REKATSNICTAQVLPAVIASMYAVYHGPVGLTRIAERTARLTAILKAGLAQLGFTHVH
                     HDSAFDTLCLKTEGATDSIAARAVQMGANLRKAWDSCLCVSLDETSTRADVELLWRIF
                     AGDARALPGFDALAATAPALIPAALRRTSGFLTHPVFNTHHSETAMLRYIRQLSDKDL
                     ALDRSMIPLGSCTMKLNATSEMIPITWPEFANVHPFAPADQRQGYAALDRQLRAWLSS
                     ITGYADVSLQPNAGSQGEYAGLLAIRAYHAAQGQGQRDICLIPASAHGTNPASAMMVG
                     LKVVVTQCDEQGNVDLADLQAQCEKHAAHLACVMITYPSTHGVFETQV"
BASE COUNT         3246 a         8013 c         7625 g         2995 t
ORIGIN      
        1 aggtcatgcc gcgatacagc acgtcgagct ggcgctgcaa caggttggag cggccgaacc
       61 agggcgagct gagctcgtgc aggctgccgg ggtgggtcag cagcgagatc aggccctgct
      121 ggaagttctt gcgcaggaac tcgggctcga aattggacag gccctcgcgc ccgaaggccg
      181 cgtaatacgc cgacacgatg gagccgcccg acacgccgct gatcagatcg gtggcgtcca
      241 gcaaggtcag gtgcttgccg ttccagtcga agcgcacctc gtccagcgca cgcagcacgc
      301 catagccaaa ggccgccgcg cgcgcaccac cgcccgaaat ggtgacggcc atcaccaggg
      361 tcggatcgcg cgtctcgttg aggtgcccgg ccctgagctg ctcgggcgcc atcggcgtgt
      421 tgatccaggg tcgcacggtc gaacaacccg tcagcagcgc caggctcagc atcaggcagc
      481 gcccgatcca ggtggccggg ctggcgcggc gtgccggggc aggcgcgcag gcgcgctggg
      541 cgaaacgtgc ttcgtcgatc atgacttgat tctatgggca gccgacgatc accagccgac
      601 gcccgagacc cccgatcgct gccgcgcggg agcgtgtgga cgtcttgttt gctatcctat
      661 tcgtagctgt cggcgcaaca cccgtgcgat ccatcggcaa tctggttttc gatgtgccac
      721 tccacgccgc cggccccagg accctgcgcg gggccagggt ggcgcgccgc gtcggcccgg
      781 cacgcccact aaactcgcgc cggtgacgcg ccctttcgtt gactttgccc gccgtcgcgg
      841 gtggctggca gccgccctgc tggcctgcct gctggcgctg cccggctgcg ccctgctgcc
      901 gagcaccgac cccgcgcgcg cagacgccag cggcgagcgc ccggccccgg cgccccagcg
      961 cagcgacacc gcacgcgacg cgccgaccgc cagcgccttc gacctgcgcg tcgagtgcga
     1021 cgacgccgag ctgcgcaccc tggtggaacg tcacgccgaa ctgctgcgct accgcgccat
     1081 caccgacctg gacgaggccg agctggcgcg cctgctggtg ctggccgaac gcgacgtgcg
     1141 ccagctgctg gccaccgagg gttacttcag ccccgtggtg aagctgcggc gcgacggcac
     1201 ccgcggcgca cggccgacgg tggtgatcgc catcgagccc ggcgccccca ccaccgtggc
     1261 gcgcgtggac atcgggttcg aaggccagat cgccagcgac gacgacgccg acgcggtggc
     1321 gcagcggcgc gccatcgtgg acggctggtc gctggaccag ggccgccgct tcacccagca
     1381 gcgctggtcg gacgccaaga ccgacgccct gcgcgccctg gtggcgcggc gctacccgcg
     1441 cggccgcatc gcctacagcc tggccgacgt ggacgccgcc ggcggccgcg cccacctgca
     1501 cctgcggctg gactcggggc cgctgttccg gctcgggccg gcgcaggtac agggcgcgca
     1561 gcgctacccg cccgagctgg ccgagcgcct gtcctggctg cgcccgggcc aggtgtacga
     1621 ccagaagcag ctggtggacg cccagcagcg cctgaccggc agcggctact acgactcggc
     1681 ctacctcagc atcgaccccg agggcgatgc ggccgccgtg ccggtcagct tcaccgtcac
     1741 cgaagccagg cgccacaagg tccagttggg cgtcggctac agcaccgacg ctggcccgcg
     1801 gctgacgctc gagcaccgcg acaaccgcgc gttcgccagt tcctggcgca gccagacgcg
     1861 cctgaacctc aatcgcaaga cgccgctgct gcaggccgag ctgacctcgc tgcccgatgc
     1921 cggcggctgg cgctggggcg cgctggcgcg ctaccagcag caggacgacg gcctgctcaa
     1981 caccctctcg cgcacgctgc gcatcggccg cagccgcatc gaggcgacgt acgaccgcaa
     2041 cgtctacctg aactacgagc acgccagcgt gaccggctcg gccacccgca ccgtgcccga
     2101 cgcgctgctg ggcgacggcg ccgcactcag cgccggctac gcctggaccg gccgccactt
     2161 cgaccgcctg ccgctgccca cccgcggctg gggcctgggt ctggacgcca gcgccggcct
     2221 caccaccgtc ggcgcgcgcc gcccgttcgc gcgcctgacc gcgcactggc tgggcatcgt
     2281 cccgctggcc ggcggcgcca gccggctggc gctgcgcgcc gaaggcggcg cggtgatcgc
     2341 cgaccggcgc gcgcgcctgc ccggcagcca cctgtttcgc accggtggcg acaccacggt
     2401 gcgcggctac ggctggcggc gcatcggcat gccggtgggc cagggcctgg tcgggccggg
     2461 ccgctacctg gcgctgggca gcgtcgaatg gcagcgcccc atcctgcaga accgctggcc
     2521 cggcctgctg gagcacagcc tgttcatcga cgtcggcagc gtggccaacc gcgccgaccg
     2581 gctgcgcccg cactggggcg tgggcaccgg actgcgcatg atcaccccgg tggggccgat
     2641 gcagctggac atcgcctacg gcctcaagtc gaaggaaatc cgcctgcaca tgacggtggg
     2701 cctggtcttc tgatggctgc gcagatccct ccggatgctc ctgattcaat agctgtcggc
     2761 gcacgaccag aaagcgctgc aagccaattt gattcacagc cgccggctcg gcgccgtggc
     2821 ctggtgcgct ggccgcagcg cctgctggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnc acagccgccg gctcggcgcc gtggcctggt gcgctggccg cagcgcctgc
     3001 tggccggcct gctggcggct gccgggctgg ccgccggcgg gctctggctg tgggccggca
     3061 gcgaaggctc gctggccacc gcgctggcct gggtcggcag gcaccagcca ctgcaggccg
     3121 aaggcgtgcg cggcacgctg cgcggcggcg gcacgctgca gcgcctggtc tggtcgcagg
     3181 acggcctgca gctgcaggcc gagcaggccg agtggcgctg gcaccccggc gccctgctgt
     3241 ggcgccgtct gcacatcgag cggctgtcgg cgcaccggct gcgcatcgac gaccagcgcc
     3301 ccgccgaccg accgaccgcc cgcccgcccg aggcgctcac gctgccactg caggtgcgcg
     3361 tggccgagct gcgggtggac gagctgcact ggcgcggccc acccgccctg cgtctggacg
     3421 acatcgccgg ccactacgcc tatgacggcg gccagcaccg gctgacgctg gagcgcgccc
     3481 gcttcgagca gggccgctac cggctgcacg ccgccgccac cgcccatgcg ccgatccggc
     3541 tcgacctggc gctgaccggc gcgctcagcg ccaccgtgcc gggcggcgcc gctgcgctgc
     3601 cgctgacgct gcaggccacg ctgcgcggcc cgctgaccga gctgcaggcg caggccgacc
     3661 tgcaggcggc ggcgcccggc accgcacccg agccgcccgc cctgccgccg ctgctcggct
     3721 ttgccgcgcg cgcgccgcag cagcagcccc gggcgccgac gccgcccgcc gcggcggcgc
     3781 aggcgtcgcc ccaggcgcgc ctcagcgccc gcctcacccc ctgggccgca cagccggtgg
     3841 ccgaggcgca tgcccgcgtg cacgcgctcg acctggccgc gctgtggtcc caggcgccac
     3901 acacgcggct gaacggggcg ctcgacctca tgcccctgcc cggcaccgcc cagaccggct
     3961 gggccttggt ggccgacctc accaaccccg ccgccggccc ctgggaccag ggccgcctgc
     4021 cggtgcaggc gctgcaggcc gatgccctgt ggcaggacga caccgcccgc gtgcgcacac
     4081 tcaaggcgcg cgccggcggc ggcaccctgg agctggacgg ccactggcgc cgcgccaccg
     4141 aagcggcgcc gccctggcag atcgacgccc gcctgagcgg cgtcgatccg gcccagctgc
     4201 acgggcagct ggccgccttc ccgatcgatg gcacggcgcg cctgcgtggc gccggccacg
     4261 ccaccgactt cgagctggcg ctgcaggccc gcaccagccc cgcgcccgcc gctgcgccgc
     4321 ctgcccgcga cacgttggcg cacgggctgc gcgcgctgcg cctgcagtcc gcccacgccc
     4381 aggggcgctg ggacgccggc ctgctgcggc tggagcgcct gcagctgcgc agcgacgacg
     4441 ccgagctgag cggccacgcc cagtggcgcc tgcagggcag cgacgcgccg ggcgggcgcg
     4501 ccgagctgcg cctgagcgca cccgggctgc gcgccagcgc ccagggcgag ctgcatgccc
     4561 agcgcggcgg cggccagctg agcgccgagc tgagcgacgc cgcccgcgcc ctggcctggg
     4621 cgcgccgcct gccgggcagc cagcgctggc tgggcgacgc ccaggcccag ggccgcgcca
     4681 cgctgcaggc gcgctggcgc ggtggctggc gcgacccgac gctggacgcc cgcctgagcg
     4741 tgccgaagct ggactggcgc ggcgccggca gcccgccgct gcagctgcgc gagctgcaac
     4801 tgacggccga cgggcggctg gcgcaggcgc ggctcgacct gcgcggccag atcgcccagg
     4861 gcgaacggcg cctgcgcctg gccctgcgcg ccagcggcgg ccgcagcacg ccggacacgc
     4921 cgctggccca ggccagctgg cgcgtgcagg tggagccatg gcaggccgag tggcacgatc
     4981 cggcgctggg cgcaggcgcc tggcagctgg ccggccaggg cgtgctgccg ctgcgctggt
     5041 cgccggcgcg cggcggccag ttcgaagccg gcgccggcac gctcaccatc acctcaccgg
     5101 ccccggcgtc gcaggcgcgg ctgagctggg agccgctgcg ctggcaggcc ggcgagctgg
     5161 acagccgcgg gcgcctgagc ggcttgccac agcagtggct ggaccgcctg gccggcacgc
     5221 cgctgcagga cgccggcctg tccggcgacc tggtgttcga cggcgcgtgg caggccacgc
     5281 tcgggcgcca gctgcggctg gacgcccgcc tggcgcgcgc gcgcggcgac ctcacgctgc
     5341 agctcaccga cgccgacacc ggcgtgcgct cgcgcgtggc cgccggtctg cgccaggcgc
     5401 agctgagcct gcgcagcgac gggccggcgc tcaccctgga cctgctgtgg gacagcgagc
     5461 gcgccggccg cgtggaaggc cagttgcgca ccgccctggt ggccggcagc gacgccgacg
     5521 gccgcacccg ctggtcctgg ccgccggacg ccgcgctggc cggcagcctg cgtgcgcagc
     5581 tgccgcagat cgccgcctgg tcgccgctgg cgccgccggg ctggcgcctg ggcggcgcgc
     5641 tggcggccga gctgcaactg gccggcacgc gcgccgcgcc gtcggtcacc ggcagcctca
     5701 gcgccgacgg ggtggcgctg cgctcggtgg tcgatgggct gcaattcaag gacggccggc
     5761 tgcgcgcgcg gctggacggt gcgcggctgc gcatcgacga gttctcgctg gccggcgtcg
     5821 gtgcgtcggg cagcggcggc cggctgatcg ccagcggcga agccgcctgg cgcgacggcc
     5881 gtccgcaggc gcagctgaac gccacgcttc agcggctgcg cgtcagcgcg cgtgacgacc
     5941 gccagctcag cgtctccggc gcgctgcagg cggcgctgga cggccgcgcc ctgcgcacca
     6001 ccggtcgcct gacggtggat caggcgctga tcgtgctgcc cgacgacagc cggccgacgc
     6061 tgggcgacga cgtgatcgtg cgcgggccgg gtggccaggt gcgcgccggc cggcaggccg
     6121 acggccgcgc gccaccgccg tcgggcgccc ccgacgcgcc tgcgcgcacg ccgctgaacg
     6181 cgcatgtcga cgtgcagatc gacctcggcc ccgagttccg cctgcgcggc atgggcatcg
     6241 acacgcggct ggccggcctg ctgacgctga ccgccgacgg cccgctgacc ggcctgccgc
     6301 acgtccgcgg cacggtgcgc accgtgggcg gcaccttcca cgcctacagc cagcagctgc
     6361 gcatcgaacg aggacaactg ctgttccagg gcgccgccga caacccggca ctggacatcc
     6421 tggccctgcg cccgcagctc ggccacgacc agcgcgtggg cgtgcaggtg gccggcacgg
     6481 cgctgctgcc gcgcgtgcgc ctgtactcgg cgccggcgct gcccgacaac cagacgctgg
     6541 cctggctgct gctgggccgc ccggcgcccg acaccggcgc cgaagccgcc atgctgcagt
     6601 cggccgcact ggccctgctg ggcgggcgcg agggccgcgg gctggcggca cgcttcgggc
     6661 tggacgagct gagcttttcc ggcaccggcg acggcgagct gaaccaggcc agcgtgaccc
     6721 tgggcaagcg cctgtccgag cgcctgtacg ccgcctacga gcacagcatc gccggcaccg
     6781 gcggcacgct gatgatcttc tacgagctgt cgcgccgctg gtcgctgcgc ggccaggccg
     6841 gcgccgagaa ctcggcgctg gacctgatct tcaagctgtc gttcgactga gcccggtgga
     6901 cggccagcgc gccagcgccg tcaaccgccg ggggcggggc tgaggcgctg gcgcagccag
     6961 tcgggcagcg gggccgattt ctgcgccgga aagttgaccc acaccacggt ggcgccgccg
     7021 gtggcgcaca ccacgccggg ctggtcggtg cgctccatgg tggcccaggt gtcgaacgag
     7081 ctgcgaccca ggtggctgac gaaggtcttg atcaggatgt caccggggta ggtgaactgg
     7141 cggatgaagt tgcagaaggc gttgacgatg accacgccct cgccgcccgg atcgggctgg
     7201 cagccgatgc cgtgcagcca gtcgatgcgc gccgtctcca tgtagcgaaa gtacaccgcg
     7261 ttgttgatgt gccccatggc gtccatgtca ccccagcgga tcgggatggt catttcaaac
     7321 accagttgct tgtgttcggg aagctcgatt ttcatggtct ggcggcggcg gcgccggtgc
     7381 gccgacagcc tgggtgaact attgaaatga tagctgctgg cgcttgctgc aaaagcgtca
     7441 gcaaccgatc agagtgcaaa accgtcgtcg gccgcgatca ccgcgccgtt gacgaaatgg
     7501 ctctggtccg aagccagcat catcagcacc gcgtccagat cggccggctc gcccacgcgc
     7561 ttgcgcggca gcatgtcgat cagtttcctg cccttctcgg tctgccagtg gtggtggttg
     7621 atctcggtgt cgatgtagcc ggggcagatg gcgttgacgt tgatgccgtg gcgcccccat
     7681 tccagcgcca tggcgcgggt catctggatc accgcggcct tgctcatgca gtacacgccg
     7741 atctgcggca gcgcgcgcag gcccgccatg ctggcgatgt tgatgatgcg cccgccggtg
     7801 aagctgcccg gcgcggcccc gcgtgcgcgc gccagcatgc gcttgcccac ttcctgggcg
     7861 acgaagaagg cgccgcgcac gttggtgtcg aacacgaagt cgtagtcctg ctcgctcacg
     7921 tcctggatgc gctgggtggt ggacacgccg gagttgttga ccaggatgtc gatcgagccc
     7981 atttcggtct cggcgtgggc caccgcggcg cggatggagt gcacgtcggt cacgtcgcac
     8041 tcgaccacat gggcatcgcc gccgtcgccc tcgatgcgcg cgcgcaggtc tttcagcttc
     8101 tccaggcgtc ggctggccag caccacgccg gcgccggccc tggccagggt gcgcgcgaac
     8161 tgcgccccca ggccgcccga ggcgccagtg accagggcca cgcggccgga caaatcgatg
     8221 ctgtagctca tggaaagcac tccggatcag aaagggtgag gaaccaggga gacagaccgc
     8281 ccgccggggc gcgagcggcg ctgcataaaa tcgcctgcac ggccgaccgg cccggtgcgg
     8341 gcgcgcgacg accatgccgc ccattatttc aaacccccac gaggaagcgc ccatgacccc
     8401 cgagcagatc atccagcagt acggcccgcg cgaagccatg gagtacgacg tggtcatcgt
     8461 cggtgccgga ccgtccggcc tgtcggcggc catccgcatc aagcagcgcg ccgccgagca
     8521 gggccaggag gtgtcggtgg tggtgctgga aaagggcagc gagccgggcg cccacatcct
     8581 gtcgggcgcg gtgatggacc cgaaggcact gaccgagctg atccccgact ggaaggccaa
     8641 gggcgcgccc ctgaaccagc ccgtgaccga cgacgcctac gtgttcctga gcgagacggg
     8701 cggcacgcgc gtgccggggg tgctgctgcc gccattcgcc cacaaccacg gcaactacat
     8761 cgtcagcttg ggcaacgtgg tgcgctggct ggccgagcag gccgaggcgc tgggggtgga
     8821 gatcttcccc ggctttgccg ccgccgaggt gctgttcaac gacgacggca gcgtcaaggg
     8881 cgtggccacc ggcaacatgg gcgtcggcaa ggacggcgaa ccgaccgaga acttccagct
     8941 cggcatggaa ctgcacgcca agtacaccat cttcgccgaa ggcgcgcgcg gccacctggg
     9001 caagcagctg atcgcgcgct tcaagctcga cgcgcagagc gacccgcaga gctacgccat
     9061 cggcatcaag gagctgtggg aaatcgaccc cagccgccac acccccggct tcgtcatgca
     9121 caccgccggc tggccgatgg acgagcacac ctatggcggc gccttcctct accacgccga
     9181 ggaccacaag gtctactgcg gctacgtggt gggcctgaac taccgcaacc cctacctcag
     9241 cccgttcgag gagttccagc gctggaagac ccacccgcgc atcaagtggt acttcaccga
     9301 cgaccgcggc caggtcaacg ccaagcgcct gtcctacggc gcgcgcgcca tcaccgccgg
     9361 cggcgtgctg gccctgccgc ggaccatctt cccgggcggt gcgctggtcg gctgcgacgc
     9421 cggctacctg aacgtcagcc gcatcaaggg cagccacgcc gccatcaaga ccggctcgct
     9481 ggccggcgaa gccgcctgcg acgccgtgct ggccgggcgc cagcacgatg aactcaccgc
     9541 ctaccccgag gccttcgacc acagctggct ggcggccgaa ctcaacaagg accgcaactt
     9601 caagaactgg ttcaagaagg gcctgacggt cggcacggtg atgaacggct tcgagcagta
     9661 cgtgctgcgc ggccacgcac cctggacgct gcaccgcgcc gaaccggacc atgtgcagct
     9721 gaaggccgca gccgagtgcg cgccgatcga ctaccccaag cccgacggca agctgacctt
     9781 cgaccgcctg tccagcgtgt tcatcagcaa caccaaccac gaagaaaacc agccggcgca
     9841 cctgacactg aaggacccgg gcgtgccggt caacgtcaac ctggcgaagt tcgccggccc
     9901 ggaaggccgc tactgcccgg ccggggtgta cgaattcgtg cccgacgaag cccgcggcgg
     9961 caacgcccag cggctgcaga tcaacgccca gaactgcgtg cactgcaaga cctgcgacat
    10021 caaggacccg acgcagaaca tcgtctgggt cacgcccgaa ggcggcggcg ggccgaacta
    10081 ctccggcatg tgagcgcctg cccgggccgc caggccgggc catggcgccg gaggccggtt
    10141 caggccaggt agggacgcag ccagcccagg ccgtcgctgg tgccacccgg caccgcgcca
    10201 cgcgccgggc ggtactcgca gccgatccag ccttcgtagc ccagccggtc gagcagctcg
    10261 aacaggtagg ggtggtgaat ttcgcccagg tccggctcgt ggcgttgcgg cacaccggcg
    10321 atctggatgt ggcccacacg acctcctggc agatggtggc ggatcttcat cgccagatcg
    10381 ccctcgacga tctggcagtg gtacaggtcc atctgcacct tcacgttcgg ccggtcgatg
    10441 gcgtcgagca gcgcgtgggc ctggtcctgg cgctgcagat agaagccggg catgtcgcgc
    10501 gggttgatcg gctcgatcag gatgtcgatg ccgtgcggcg cggcctgctc ggcggcgccg
    10561 cgcaggttgg cgacataggt ggcgcgcagc gtggcggcgt cgacacccgc cggcatcaaa
    10621 ccggccatca cgtggatgcg cgggcaatcc agcgcgcgtg cataggcgat ggcgcgctcc
    10681 aggccggcat ggaattcgcc ttcgcgcccc gggatgcagg ccaggccgcg ttcgcccgcg
    10741 tcccattcgc ccggcggcgc gttgaacaac acctgccgca gcccgtgctg gcgcagcagc
    10801 gcgctcagtt gctctggcgc ccaggcgtag ggaaacaggt attccaccgc cgtgaaaccg
    10861 tcgctggcgg cagcagcaaa gcggtcgagg aaatccagtt cgttgtagag catcgacagg
    10921 ttggcggcaa agcgcggcat gggtggttcc tcaccagaag gcgttgaagg cgcggcgcag
    10981 ttcgtcgatc tgcgccgtgt tcaggggggc ggccggtgcc agcaaccaca gccgggcggt
    11041 ttcttccagc tcctccagcg tggccatggc ggcggccggg gtgtcgtgcc agacgttggg
    11101 gcccagccgc gccagcatca cggcgcggat cgggtgaccg gcttcggcat gccgggcgat
    11161 ggcccgggcc acggcctcgg cggcctcggg cgcgccaggc cggtggtagg ggatatgggg
    11221 cacgcggccg accttcatca cgaaataggg cgtgagcggc ggcagcagtt cggccgccgc
    11281 cgggtcggcc cgcagcgagc aggccaccag atgggtgctg tggctgtgga ccacgcagcc
    11341 ggccggcgcc gccgtgccgg cgctggcctc gtagatgcgc cggtgcagcg ccagggtctt
    11401 gctggcgggg tcgccgccgg tctggcgccc ctgggcgtcc agtcgcgcca gtcgggcagg
    11461 gtcgagtgtg cccaggcagg cgtcggtggg cgtgatcagg tagccgtcgg ccagccgcac
    11521 gctgatgttg ccggccgtgg catgcacgta gccgcgctcg aacagcgagc ggccgacgcg
    11581 cacgatttcc tcgcgggccc gggcctcgct catgggcgcg gggctcatgg ccgcagcacc
    11641 gcgaacgccc gggtgaagaa atcctcacca ccgaaattgc ccgatttgag ggcgatgtgc
    11701 aggccctgcg gcgccagcgg ggatgccgcg tggcaccatg gcacgcccgg gtcgatctgc
    11761 gcgccgatgc gcagttgcgc gacgcccagc gcctgcacgc aggcgcccga ggtttcgccg
    11821 ccggccacca ccagttggcg cacgcccagc ccgaccagcc cgcgcgcgat ggccgccagg
    11881 gtctgctcca ccagctcgcc cgcccgcgcc acgcccaggc gctgctggat ggcctgcacg
    11941 gcggccggct cggcggtcga atacaccagc agcggcgcgt ccgcggccag ccgtccctgc
    12001 gcccagtcga gcgcctggcg caccaccgcg tcgccggcgg ccatgcgcag cggatcgagc
    12061 gccagcgccg ggtgaccgca ggcgatgaaa tgcgccacct gggcctgggt ggcctgtgag
    12121 cagctgcccg ccagcaccgc ccgcaggccc gacgcggcgg gcagcgcggc ggcctgcgcc
    12181 gccggcgcca gtccgaaatt ggccggcagc gcgatcgcca cgccggagcc ggcagtcacc
    12241 agcggcagct ccgccagcgc cgggccgagc cgccacaggt catcgttgga caccgcatcg
    12301 acgatggcga tgcccacccc ttcggcgcgc aggtcggcaa tgcgggcgcg gatggcgtcg
    12361 ctgccggccg ccagcgtgcg ctgctcgatc aggccgaccc gccggcgcgt ctgcgcctgc
    12421 agcacgcgca ccaggttggc gtcggtcatg ggcgtcagcg ggtgctggcg catgccgctc
    12481 tcgctcagca gctggtcgcc gacgaacagg taacccttga acacggtgcg cccgttgtcg
    12541 ggaaaggccg gcgtggcgat ggtgaagtcg cagtccagcg cctgcatcag cgcctcggtg
    12601 acgggtccga tgttgccggc cggcgtgctg tcgaaggtgg agcagtactt gaagtagatc
    12661 tggcgcgcac cctgcgcccg cagccagcgc agcgccgcca gcgactgcgc caccgcctgg
    12721 tcggcggcga tggtgcgcga cttcagggcc accaccacgg cgtcggcggc ggcgtcggcg
    12781 gccagcggcg cctcgggcac gccgatggtc tgcaccacgc gcatgccggc gcgcaccagg
    12841 ttgttggcca ggtcggtggc accggtgaag tcgtcggcga tgcagcccag caggattggg
    12901 ggcgtcatgg gcgctccttg tcggtcggtg ccggcagttc gatgccggga aaaatcttga
    12961 tcaccgcgct gtcgtcctcc agcgcgtggc cggcgctgga agcctgcatg aacatctggt
    13021 gcgcagtagc cgccagcggc agcggaaact tgctggcgcg cgccgtgtcc agcaccaggc
    13081 ccaggtcctt gacgaagatg tcgaccgccg acagcggcgt gtagtcgccc gccagcacgt
    13141 gggccatgcg gttctcgaac atccagctgt tgccggcgct gtgggtgatg acctcgtaca
    13201 aagccgccgg gtccacgccc tcgcgcaggc ccagcgccat ggcctcggcg gcggcggcga
    13261 tgtgcacccc cgccagcagt tggttgatga tctttacttg gctgccggca ccggcgcgct
    13321 gaccgaggcg atagaccttg ccggccatgg cctccagcac cgcatcggcc gcggcgtagg
    13381 cggccggtgt ggcggccgtc atcatggtca tctggccgct ggcggcacgc gccgcgccgc
    13441 cagaaatcgg gccgtccacg tacaggatgc catgctgtgc caagcgggct tcgagggcca
    13501 tcgaccagtt gggatcgacg gtggagcaca tcacgaacac gctgcccggc gccatggccg
    13561 cagcagcacc atcgctgccg aacagcacct gctccgtctg ggcggcgttg accaccacgg
    13621 agaccaccac gcgcacgccg ttcgccacct cagccggtgt ggtgcaggcc acgccgccct
    13681 cggcagcaaa ggccgccgcc acgccggcac gggcgtcgca ggcacgcacc cgatagccgc
    13741 gctgacgcag cgtgcaggcg atgcccaggc ccatggcgcc caggccgatc acgccgacgt
    13801 gtcgatcact catggaaggg ttcctcgtgc cggtgcctgc cggtgggtcc ggcggcagcc
    13861 gggattctga cccgaaaccc ggcctgcgcg ccacgcgccg gccgcaccgg gccggcgcct
    13921 atcataggca tccgcacggc acacctgccc cgccgtgcct gcaccgagga gacgaacatg
    13981 cacatcgtga tcaccggcgg cgccggcttt ctgggcgtgc ggttggcgcg cgcactgctg
    14041 cagcgcgacg cgctgtcgct ggcgggtgcc gcgccacgcc cgctcacccg gctgaccctg
    14101 gtcgaccgcg tggcgccgcc ggcggcgctg acgcaggacc cgcgtgtgcg cgtggtgacc
    14161 ggcgagctgg acgcgctgct gacgccggac gccagcggtc agacgcgcgc cgtgccgacg
    14221 gacagcgacg ccgtggtgca tctggcggcg gcggtgagcg gcgaatgcga ggccgacttc
    14281 gacctcggcc tgcgcagcaa cctggacgcc acgcgcgccc tgctgcagac ctgccgggcc
    14341 ctgggcacgc ggccgctgtt cgtgttctcg tcctcgctgg cggtattcgg ccacacgccg
    14401 gagcagccga tgccggccat catcgaggac gacaccctgc cgacaccgca aaacagctat
    14461 ggcacgcaga agtacatgag cgagctgctg gtggccgact gcacccgcaa gggtttcgtg
    14521 cgcgggcgca acgtgcgcct gatgacggtg agcgtgcggc cgggccggcc caatgcggcg
    14581 gcgtccagct tcctgtcggg catcgtgcgc gaacccctgg ccggccagcg cgccgtgtgc
    14641 ccggtggcgc ccgacacccg ggtggcgctg tcgtcgcccg cgcgcaccat cgaaggcctg
    14701 gtgcgtgcgc tggaggccag cgacgaagac tggggaccgc gcaccgcgct caacctgccg
    14761 gccctgtcga ccaccgccgg cgacatggtg gcggcgctgg cgcgcgtggc cggccccgag
    14821 gtggcggccc gggtggactg ggtggccgac gccggcatcg cccgcatcgt cggcgcctgg
    14881 ccggccggca tgaacgcgcg ccgcgccgcg cggctgggcc tgcgggcgga cgccagcttc
    14941 gacgacatca tccgcgacca cctgcgcgaa aacccggtgc cgcccgcagc agcggcacgg
    15001 ccataatgac cggccgtggt cgccgccgct cggggcgtgc cgaccggcat tcatcatcga
    15061 tctgaggagt cgtatccatg aagttgcacc aactggccct gggcctcggt ctggcactgg
    15121 gactggtggc gggcgcgcag gcgcagacca ccatgaagat cagcatttcg gtggcgcaga
    15181 actcgcacca gggcatcgcc atcgacacct tcgccaagga ggtcgaaaag cgcaccggcg
    15241 gtcgctacaa gatccagacc ttctacagcg gcgccctggg cggcgagcgc gaatcgatcg
    15301 aggcggtgca actgggcacg caggagctgg cgttcagctc caccggcccg gtgcccaact
    15361 tcgtgcccga ggtcaagatc ctcgacgtgc ccttcctgtt ccgcgacaag gcgcatgcgc
    15421 gcgccgtgct ggacggcccg atcggccagg acatgctggc caagttcgag cccaagggct
    15481 tcaaggcgct ggcctgggcc gagaacggct ttcgccacat gaccaacagc aagcgcgacg
    15541 tcaagacgcc ggaggacctc aagggcctga agatgcgcac catggagaac ccggtgcacg
    15601 tggcggccta caagggcttc ggcatcatca ccacgccgat ggccttcccg gaggtgttca
    15661 ccgcgctgca gcagggcacg gtggacggcc aggagaaccc gctgtcggtc atcatctcgg
    15721 ccaagttcga ccaggtgcaa aaacacctga cgctgaccgg ccacgtctac tcgcccgcca
    15781 tcttcctgat gaacaagggc gccttcgaca agctgagcgc ggccgacaag caggccttca
    15841 tcgaagccgc caaggaaggc accaaggcca accgcgcgcg ggtcgacgag gacgacgcca
    15901 agggcgtggc cgacctgcgc gccaagggca tgacggtgat cgacaccatc gacaaggcca
    15961 agttcgtgtc ggcgctggcg ccggtcaatg cccagttcga gaaggacttc ggcaaggcca
    16021 acctggaccg catccgcaac ttcaagtgag ctgccggcgg cgctggcgtc gcctcgcaga
    16081 atactattgt tttgatagct tctggcgccc gactgtcctg cgcagcgggc gttttttatc
    16141 ataggttcgc ggcatgaaag cgttcatcct gaccctggag ggctggacca cccgcatcgc
    16201 catggtgctg gcctgtctga tgctggcgct ggccgcggca ctgggggtgt tccagatctt
    16261 cacccgcttc gtgctggagc agccggccga gtggagcgag gtgctgatcc gcttttcgct
    16321 catctggatg gtgttcctcg gcataccgat ggcgttccgt cagggcgcca tggtgagcgt
    16381 ggacgcgctc taccgcgccg tgcccaccgg tgccaaacgg gtgctggacc tggtggtggc
    16441 gctggcggcg ctggcgctga tgctgatcgt gctgtggtgg ggctgggact acgcggtgcg
    16501 cggcagcgtg cagaccatgg cggggctgga atcgctgtcg atgttctggg cctacctggc
    16561 gatgccggtg ggcgccgcgt tctcgctgct gggcattctg gccaactacc tggatccgcg
    16621 ccgcatggaa ctggagaccg cccaatgaac gccgccccat gccgcttcgg gtcaacccgc
    16681 cggcccctcg gccatgacag ggcgcgcgga ggtgccgcat gacttccgcc atgctgatca
    16741 ccatggtgct gggctttgcg ctcagcgtct cggttgcggt gtcgatcggg ctggcggcca
    16801 tcgtcggcat ccaggccagt caggcgcaca tgctgatttc ggccaaggag atgttcaacg
    16861 ccatcaacaa gtttccgctg gcagcgatcc cgtttttcat cctggccggc aacctgatgg
    16921 aaaccggcgg catctcgcgc cggctggtgg aattcgccaa gagcctggtg ggcggtgtgc
    16981 agggtggcct gcccatgacc tgcgtgctga cctgcatgat ctttgccgcg gtgtcgggct
    17041 cgtcggtggc caccaccttc gccatcggcg ccatcctgat tccggcgctc atcaagcacg
    17101 gctacccgac cacctgggcc gccgcactgc aggccaccag cgccgagctc ggcgtcatca
    17161 ttccgccctc gattccgatg atcctgttcg gcgtctcggc cgaggtgtcg atcggcgagc
    17221 tgttcatcgc cggcttcggc cccggcctgc tgatttcggg cgcgctgatg ctgttcgtgt
    17281 ggctctactg caagtggcag ggctggggca ggaacgacgg cgacgggcgt ctgggtgtcg
    17341 tggccgccac gcgccaggcc gcctgggcgc tgctgatgcc ggtcatcatc ctgggcggca
    17401 tctacggcgg catcttcacc cccaccgagg cctcggcggt ggccgtgctg tacgccctgg
    17461 tggtgggcat gctgatctac cgcgaaatcg gcgtcaagga cctgtacgtg acgctgcgca
    17521 agtcggtggt gtcgtcggcg gtgatcatgt tcatcatcgc caacgccggc ctgttcgcct
    17581 tcctgatcac gcgcgccggc gtgcccgaag ccatcggcca ctggctggaa caggtgctgc
    17641 agaaccccgc cctgttcctg ctgggcgtga acgcggcgct gttcgtgatc ggcatgttca
    17701 tcgagaccag cgccgccatc atcgtgctgg cgccgatcct ggtgccggtg gcgcagcgct
    17761 tcggcatcga cccggtgcat ttcggcctgg tgatggtggt caacctggcg ctgggcatga
    17821 tcacgccgcc gttcggcgtc aacctgtttg ccgcctgtac cgtggcgcgc gtgtcgctcg
    17881 acaagatcat ccgccacctg gtgcccttcg tgggcgtggt gctggcctgc ctgatggtga
    17941 tcacctacgt ccccacgctg tcgctggcgc tgcgcgatct ggtgtaccgg tagtaccggt
    18001 aggcgtcggg gcgctagaat cgcgccgtca ggagagagcg cccgcagtcc aggcgccgcc
    18061 gaaggccgaa cgctcaggca aaaggactgg cacacatgat tctgctggag agaggccgcc
    18121 cacaccggcg gcccaccgaa ggggcaaggg ccaaccggct cgaatctctc aggtaaagag
    18181 gacagcaggg ttccaggcgg ggccagcgtg ccttgccgat gccccccatc gcattccagg
    18241 agccccgccc atgtccgccg ccgacagccc cctgctgaaa accccgctgc acgccctgca
    18301 cctcgaactg ggcgcgcgca tggtgccctt tgccggctac gagatgccgg tgcagtacca
    18361 ggctggcctg atcgccgaac accgccacac gcgcgaggcg gccggcctgt tcgacgtctc
    18421 gcacatgggc cagctcaagc tgatcggccc ggatgccgcc gcagcgctgg aaaccctgat
    18481 gccggtcgac gtgcagggcc tggccgagaa caagcagcgc tacggcctgc tgctgaccga
    18541 cgagggcacc atcatcgacg acctgatgtt cgtcaaccgc ggtgaccacc tgttcctgat
    18601 tgtgaatggc gcctgcaagg ccggcgacat cgcccacatg caggcgcgca tcggcagccg
    18661 ctgcctggtg cagcccctgc ccgatcaggc cctgttggcc ctgcagggcc cgcaggccgt
    18721 cggcgcgctg cagcgcctgg tggccggcgt cgagaagctg gtgttcatga ccggcggcgc
    18781 cttcgactgg ctgggcacgc ccttgtacgt gacccgcagc ggctacaccg gcgaggacgg
    18841 tttcgagatt tcgctgcccg gcgagcgcgc cgaggccttc gcccgcgccc tgctggcgca
    18901 gcccgaggtc aagcccatcg gcctgggcgc acgcaattcg ctgcgcctgg aagccggact
    18961 gtgcctgtat ggcaacgaca tcgaccacac caccagcccg gtcgaagccg gcctgaactg
    19021 ggccatgcag aaggtgcgca ggagtggcgg cgcgcgcgcc ggcggctttc cgggggcggc
    19081 gcgcattctg gcccagctcg acggcacgca gccggtggaa cgccagcgca tcgccctgat
    19141 ggcccgcgag cgcgtaccgg tgcgcgaacc ggccgaactg cgcaacctgg acggccagca
    19201 ggtcggccag gtcaccagcg gcctgctcag cccgacgctc gacaagccca tcgccatggg
    19261 ctacgtgcgc cccggtttcg ccaccatcgg caccgagctg ttcgccatgg tgcgcggcaa
    19321 gccggtgccc atggaggtgg tggccacgcc cttcgtgccg aaccgctacc accgcggttg
    19381 acccaagcaa aaccggccgc agcgcccgtc caacctgcgc cagcaactca tttttcagga
    19441 gcaaacatga gcctcaagta caccgaagac cacgaatggc tgaagatcga gggcgacgtc
    19501 gccaccgtcg gcatcaccca ccatgcgcag gacgcgctgg gcgacgtggt gttcgtcgag
    19561 ctgcccgagg tcggccgcca ctatgcccag aaggacgcgg ccggcgtggt cgagagcgtc
    19621 aaggccgccg ccgacgtcta catgccggtc agcggcgaag tgctcgaagt caacgaggcg
    19681 ctgcgcgaag accccagcct ggccaacagc gacccgcagg gcgccggctg gttcttcagg
    19741 attcgcctga gcgagccggc gcaggccggc gcgctgatgg acgaagccgc ctacaccgcc
    19801 ttcagcgccg gctgaagccc gggcgcgctg ccgccccacc cgcgccacgc ccggagcgcg
    19861 gcgccgcgcc gccgacgcag ggccaccccc acggcctgtg cttgcgcgcc tgcagcgcac
    19921 ccgccgcgcc cgcgccaacc ccctttcctt cgccgcttgc atcgatgcca cccatgagcc
    19981 agcccaccct gcccgaactg gaaaaccccg gtgaattcat cgcccgccac atcggcccca
    20041 gcccggccga ggaggcccac atgctgtcgg tcatcggcga ggcctcgcgc cgggcgctga
    20101 tcgactcgat cctgccgccg gacatcgcgc gccgcacgcc gatggatctg ccgccggcct
    20161 gcagcgaagc cgacgcgctg gccgaactca aggccatcgc cagccgcaac cggcaccaga
    20221 tgaagagttt catcggccag ggctactacg gctgccacct gccgggcgtg atcctgcgca
    20281 acatcctgga gaaccccgcc tggtacaccg cctacacgcc ctaccaggcc gaaatcagcc
    20341 agggccgcat ggaggcgctg gtcaacttcc agaccatggt gaccgacctg accggcatgg
    20401 acatcgccaa cgcctccatg ctggacgaag ccaccgccgc cgccgaggcc atgacgctgg
    20461 cacgccgcac cgtcaaggcc aagggccagg tgatcgtgct gtcgggcgac tgccatccgc
    20521 agaccatcga ggtcatgcgc acgcgcgccg cgccgctggg cctgacgctc aggctggcca
    20581 actcggccgc cgagtgggac gccgccatcg ccgccgacga ctactttgcc gccctgatcc
    20641 agtaccccgc cagcagcggc tggctgatgg actggagcga cgaggtcgag aagatccacg
    20701 ccaagggcgc ggccgccatc tttgccgccg acctgctggc gctgaccctg ctcaagacgc
    20761 cgggcgagat gggcgccgac atcgtggtgg gcagcaccca gcgctttggc atgcccatgg
    20821 gcgcgggcgg cccgcacgcc gcctacatgg cctgccgcga cgattacaaa cgctccatgc
    20881 ccggccgcct ggtcggcgtc agcgtggatg cgcacggcca gccggcctac cggctggcgc
    20941 tgcaaacgcg cgaacagcac atccgccgcg aaaaagccac cagcaacatc tgcaccgcgc
    21001 aggtgctgcc ggcggtgatc gccagcatgt acgcggtcta ccacggcccc gtcggcctga
    21061 cgcgcatcgc cgagcgcacg gcacgcctga cggcgatcct gaaggccggt ctggcgcagc
    21121 tgggcttcac ccacgtccat cacgacagcg ccttcgacac gctctgcctg aagaccgaag
    21181 gcgctacaga ttcaatagct gctcgcgcag ttcagatggg cgccaacctg cgaaaagcct
    21241 gggattcctg cctgtgcgtg tcgctggacg aaaccagcac ccgcgccgac gtcgagctgc
    21301 tgtggcgcat cttcgccggc gacgccaggg cgctgcccgg gttcgacgcg ctggccgcca
    21361 cggcgccggc gctgatcccg gccgcgctgc gtcgcaccag cggctttctc acccacccgg
    21421 tgttcaacac ccaccattcc gagacggcca tgctgcgcta catccgccag ctgtccgaca
    21481 aggacctggc gctggaccgc agcatgatcc cgctgggcag ctgcaccatg aagctcaacg
    21541 ccaccagcga gatgatcccc atcacctggc cggaattcgc caacgtccac ccgtttgccc
    21601 cggccgacca gcgccagggc tatgccgcgc tcgaccggca gctgcgcgcc tggctgtcgt
    21661 ccatcaccgg ctacgccgac gtcagcctgc agcccaacgc cggcagccag ggcgaatacg
    21721 ccggcctgct ggcgatccgc gcctaccacg ccgcgcaggg ccagggccag cgcgacatct
    21781 gcctgatccc ggccagcgcc catggcacca acccggccag cgccatgatg gtgggcctga
    21841 aggtggtggt gacccaatgc gacgagcagg gcaacgtcga cctggccgac ctgcaggccc
    21901 agtgcgaaaa gcacgcggct cacctggcct gcgtgatgat cacctacccc agcacgcacg
    21961 gcgtgttcga aacccaggt
//
LOCUS       DAOQTZ010000040        21932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2794_length_21932_cov_13.732047, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000040 DAOQTZ010000000
VERSION     DAOQTZ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 21932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21932
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2794_length_21932_cov_13.732047"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1798
                     /locus_tag="PKN51_06590"
     CDS             110..1798
                     /locus_tag="PKN51_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNT84803.1"
                     /translation="MPPQLARHLPFLRWPRPTARLLQGEAMAGLTVGLMVIPQGVAYA
                     QLAGMPLITGIYASMLPALVAVLFSASARLSVGPTALTSLMIAAALTPLATPGSAEWV
                     ALAVWLALLSGLLQVALGLVRFGWLLNLVNAPVLMAFTQAAAVLIIGSQLPALLGFRA
                     GWADMLRQPAFDPLASAFGLGALALLLLGRRWKPTFPGVLVIVVAAAALSWALGFEAG
                     GGAVVGALPQGLPAPYLPPWPGWQLLGQLLLPTLVVTLVSFLETAASAKVDSQRKGER
                     WDQDQDLIGQGLAKIASGLCGAFATSSSFSRSALNLYAGAQTGWATVVSVAVVLLALL
                     LFTPVLRFVPQAVLAAIVVAAVLGLIKPREFLRLWRVSRVETVIAALTFAVTLAAAPA
                     LYWGVLTGVLMALSLFLYQRLHPRIIEVGLHADGSLRDRHLWQLAPLAPRTLALRLDA
                     ALDFASAGQFERHMTEYLAEHPTTRHVCLFAQPINRIDATGAEVFARVLGQLHERRVT
                     LLIVGLKLPAERVLRAAGALGEWPLLQMVRTDAEALAALARLEEPPADIAAAAI"
     gene            complement(1901..2782)
                     /locus_tag="PKN51_06595"
     CDS             complement(1901..2782)
                     /locus_tag="PKN51_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT84804.1"
                     /translation="MSAQALALVLLAGLVHAGWNIVAKQSGGDARFAFFTSVLMALAW
                     APLGLWLGWQALPHWDAAAWACVAASGLLHVLYYVTLLRGYRVADLTVVYPLARGSGP
                     LLSSLVAVTLLGERLSPLAAAGIGAVVLGVFLIAGGPRLLRRRDDPQARARVRQGLRY
                     GLLTGVWIASYTVVDGYAVRWLALSPILLDYMGNWVRIGLLLPVALRDRAATAQLWRR
                     QWRGALVVALCSPVAYVLVLFAMQQAPLSLVAPAREVSMLFAALIGGQLLGEGDRGVR
                     VAGALCIAAGVAALGLG"
     gene            complement(2798..3220)
                     /locus_tag="PKN51_06600"
     CDS             complement(2798..3220)
                     /locus_tag="PKN51_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HNT84805.1"
                     /translation="MLVRLLFASRAVDASREAIESILASARRHNPESGITGILLHGSG
                     VFLQGMEGGRQAVSDLYGTIQRDPRHKDVVLLHYEEILERRFGGWTMGLVDVGRVNAS
                     VLLKYSERATLDPYAVSGRMSMALLEELMATACIASHA"
     gene            complement(3226..3951)
                     /gene="folE"
                     /locus_tag="PKN51_06605"
     CDS             complement(3226..3951)
                     /gene="folE"
                     /locus_tag="PKN51_06605"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HNT84806.1"
                     /translation="MKAQPEFPTPAGAEDEGTPVSVRIRQRLQAAQRRYHANDNIADC
                     IRPGELEQLLDEVTTRMEGVLDSLVIDTAGDHNTRDTARRVAKMYLNEVFKGRYVKAP
                     SITEFPNAERLNELMIVGPITVRSACSHHFCPVIGQLWIGVLPNEHTNVIGLSKYARL
                     AEWVMGRPQIQEEAVVQLADYIQSKTRPDGLAIVMQATHYCMAWRGVKDMDSKMINSV
                     MRGVFLKDMNLRREFLALIPQKS"
     gene            complement(4027..4806)
                     /locus_tag="PKN51_06610"
     CDS             complement(4027..4806)
                     /locus_tag="PKN51_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNT84807.1"
                     /translation="MREVHFGGAHQLLRVATYNIHKGVQGLGPARRLEIHNLALAIET
                     LDADIVCLQEVRAINRKEASYFTGWPELPQAEFLAPEGYDAVYRTNAVTRHGEHGNAL
                     LSRWPIIHERHEDMSDHRFEQRGLLHAELLVAGVSVHAIVVHFGLIPASRIRQTAQLK
                     AYIAREVPRGAPLIVAGDFNDWGTRVRRLLRMEALHSFEGPRTLTYPSRYPVAQLDHI
                     YARGLHPLSLAAPRGRVWSRMSDHLPLIAEFRMPHDALHKS"
     gene            complement(4803..5294)
                     /gene="nudB"
                     /locus_tag="PKN51_06615"
     CDS             complement(4803..5294)
                     /gene="nudB"
                     /locus_tag="PKN51_06615"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HNT84808.1"
                     /translation="MSAHSCVGRCRFKIPESVLVVIHTPALEVLLIRRADVRQGEFWQ
                     SVTGSKDDPGEALTQTAVREVGEETGIDARAPGCLLRDWGIRNRYEIYPRWRHRYAPG
                     VTHNLEHVFGLCLPARAPVRLNPREHDAFEWLPWRAAADRCFSPTNAEACLLLPRFAG
                     APA"
     gene            complement(5324..5659)
                     /locus_tag="PKN51_06620"
     CDS             complement(5324..5659)
                     /locus_tag="PKN51_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84809.1"
                     /translation="MNRPPTDDLRARREQLRLRSDQLREQIAARTQVLQPLFRASDRV
                     RSGVRGVQQARQGRAPYVLGLLALSALAGALLVRPRLATQLALRAWSGWQMYRRARPV
                     VERALRQWR"
     gene            complement(5656..6039)
                     /locus_tag="PKN51_06625"
     CDS             complement(5656..6039)
                     /locus_tag="PKN51_06625"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNT84810.1"
                     /translation="MTSPAQDAGTAARLRALLADVVELVQVRLELFTVEAREELARLA
                     RMAVLGALAVVLLSFGLIFLALFLTVLLWDSQRLLALGVFTTLFLGGGAGLVLWVRQQ
                     ARQGLQMFAATRDELLRDQQRLRQP"
     gene            complement(6068..6397)
                     /locus_tag="PKN51_06630"
     CDS             complement(6068..6397)
                     /locus_tag="PKN51_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HNT84811.1"
                     /translation="MPHSADLIESMTETKDQLASNLRRVVSDTEDLLAATAEHTDSRI
                     AELRARARENLRIARDKLADADAVVRARARQAAAATDDYVHDNPWSSIGAAAALGILI
                     GVLLGRR"
     gene            complement(6491..6946)
                     /locus_tag="PKN51_06635"
     CDS             complement(6491..6946)
                     /locus_tag="PKN51_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNT84812.1"
                     /translation="MIKHFETLAELAACVGQEVAVTDWFTVEQAHIDQFAQATGDHQW
                     IHVDPERAKAGPFGDPIAHGYLTLSMLAGHLGGVVQVREVRMGVNYGLNKVRFMAPVP
                     VGSRLRARVTLLDAQPLEGGMQLAWRSTIEREGADKPVCVAEPLARYYV"
     gene            complement(6984..7610)
                     /locus_tag="PKN51_06640"
     CDS             complement(6984..7610)
                     /locus_tag="PKN51_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNT84813.1"
                     /translation="MPVVVIANPKGGVGKSTLASHVAGYWASRGHAVMLGDVDRQQSS
                     RLWLSLRPEAASPIATWDVLRDFVVRPPRGTTHVVLDTPAGLHGSKLTELLRLADKVL
                     VPLQPSIFDIYATREFIDELLGARGARQAELALVGMRVDERTIAAEQLRQFTDGLGLP
                     VLGLLRPTQNYVHLAARGLTLFDIAPGRVARDLEQWQGICRWLDRSPA"
     gene            7757..8839
                     /locus_tag="PKN51_06645"
     CDS             7757..8839
                     /locus_tag="PKN51_06645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNT84814.1"
                     /translation="MTTPPLTLADQAAQAERLLDPAVWAYLNGGAADELTLRANLTAW
                     QRLPLWPRVLRPLGGGHTRSVLAGRELAHPILLAPVALQRLAHPDGERASALAAAAQG
                     AGYVLSTQSSTPLETVAQAVLGDPGRGPLWFQLYWQADRGFNRALVQRAEAAGFEAIV
                     LTADAPVQGVRDRERRAGFVPPPGVRAVHLQGAPLAPPAADTYCAGLPAHAPNWDDLA
                     WLQGQTRLPLWLKGVLHPQDARQAQALGVAGLIVSNHGGRTLDSAPASADALVQVRAA
                     VQDALPLIVDGGIRRGTDVLKAIALGARAVLIGRPALHALAAGGALGVAQMLRLLRDE
                     LEMAMALTGCRTLADAGPALLTPTAA"
     gene            9057..9281
                     /locus_tag="PKN51_06650"
     CDS             9057..9281
                     /locus_tag="PKN51_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNT84815.1"
                     /translation="MIVCVCNCISDKEIARQAHAGMGFDDIQLELGVATCCGRCEDFA
                     RDLVAQCSASRPVAALRDAGGASAGLMEAV"
     gene            9458..10156
                     /locus_tag="PKN51_06655"
     CDS             9458..10156
                     /locus_tag="PKN51_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNT84816.1"
                     /translation="MSLPGRHTVVVGSVLLMHGVALWALHQGLLRPPVELIVPAQVLA
                     EFITPQPAPPPPAPPPPEPSKPTPAAVKPAPRPTPRPRPAPQPQPQPLAVQQAAPSAP
                     VMPIAPPEPPAPAPVAAPAPAPAAPPTPPAPPAPPAVVQPSSNAAYLNNPKPAYPAMS
                     KRLGESGRVVVRVLIGPDGRAQQAHIQRSSGYERLDQVALETARDRWRYVPGTRGGVP
                     EAMWFNVPINFVLE"
     gene            10164..10877
                     /locus_tag="PKN51_06660"
     CDS             10164..10877
                     /locus_tag="PKN51_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT84817.1"
                     /translation="MNNEFGLLHVWAQGDWVTRSVFGALLLMSVASWVVIVLKALGLM
                     RTQRQAARVEGFWHSSDIAEGLAKLGTGEANPFRALAIEGREAAAHHRATQAQLHDSL
                     DVSDWISRALQGSIDATASRLQAGLAVLASVGSTAPFVGLFGTVWGIYHALMKIGAAG
                     QASIDQVAGPIGEALIMTALGLAVAIPAVLGYNALVRGNKTLLMRLGRFAHDLHAYYV
                     TGSRVNGSGDGKVVAMKKA"
     gene            10883..11302
                     /locus_tag="PKN51_06665"
     CDS             10883..11302
                     /locus_tag="PKN51_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNT84818.1"
                     /translation="MAGFIAQDHDEVMSEINMVPFIDVMLVLLVIFIITVPVMKHAVN
                     VQLPRAANEKEVVQPQTLRLTVQADGSYWLDDQKLADDQLGTRLQAAAAQQPQPDLHI
                     RGDRDVRYERVAQAMAAAQQAGLSKIGFVTEPPQRTP"
     gene            11299..11523
                     /locus_tag="PKN51_06670"
     CDS             11299..11523
                     /locus_tag="PKN51_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HNT84819.1"
                     /translation="MNGQTATLTPARRAADTTPAHHAPDRPRPAAEAAAPAPLPSEQL
                     LQGRKTIEISHNGTLYRLQATKLGKLILTK"
     gene            complement(11683..12426)
                     /locus_tag="PKN51_06675"
     CDS             complement(11683..12426)
                     /locus_tag="PKN51_06675"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNT84820.1"
                     /translation="MNSEIFNGWPGWLTQHSGPWLPLLSTGVALLALMLLWAMVRRSW
                     RRGRAARNYDASADRFGQTPAITERQVELLRYLQQAFGDGVVLYRPRLASFLAVRRTR
                     DRLAAQQRLLEQQVDFLVCADDGKPLFAFEVDAFKDPAAGGPDRDASEKNRMLKSAGV
                     RLVRLKGALASLPPPEILRMRLLAAQRTPVPAAEPAASDFKASGFASSGFAASGFAHS
                     RSADSGVMSLSALMALPDGEDPWSGVRKR"
     gene            complement(12609..13700)
                     /gene="apbC"
                     /locus_tag="PKN51_06680"
     CDS             complement(12609..13700)
                     /gene="apbC"
                     /locus_tag="PKN51_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNT84821.1"
                     /translation="MTILEQDVRRAAATVLDPNTQQDFVSTGQLKNLQISGGDVSFDI
                     ELGYPARSQMPALRQALVAAVRTLGGVGNVSVNIVTRVVAHAVQRGVPLLPGVKNIVA
                     VASGKGGVGKSTTAVNLALALAAEGASVGILDADIYGPSMPIMMGLSGRPESLDGKTM
                     EPLTNHGVQVISIGFLVDPDQATIWRGPMATQALDQLLRQTHWKELDYLIVDLPPGTG
                     DIQLSLSQRVPLTGAVIVTTPQDIALADARKGVAMFEKVGVPILGLVENMAVHVCSNC
                     GHVEHIFGADGGKRYAADKGIEYLGALPLALEIREQADSGRPSVVADPDGQVATSYKA
                     IARQVAIRIADRAKDYSSKFPSITISRSS"
     gene            13903..15984
                     /gene="metG"
                     /locus_tag="PKN51_06685"
     CDS             13903..15984
                     /gene="metG"
                     /locus_tag="PKN51_06685"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNT84822.1"
                     /translation="MTARQLFVTTALPYANGKFHIGHIMEYIQADIWVRFQRMMGAEV
                     NFVGADDAHGAPIMIAAEKAGKTPEQFVADIAAGRQQYLDGFLIGFDNWHSTHSPENT
                     AISQAIYTDLKAAGLIESRTIEQFYDPQKGMFLPDRFIKGECPNCHSKDQYGDNCEVC
                     SAVYAPTELIEPYSALTGARPELRTSEHFFFRLSDPRCIEFLRGWTERLQPEVANKVR
                     EWIEPGADGQPKMGDWDISRDAPYFGIPIPDAPGKYFYVWLDAPIGYLASLKNLLDKR
                     GQDWAAYMANPALEQYHFIGKDIITFHTLFWPAMLKFSGRKVPDNVFVHGFLTVNNGE
                     KMSKSRGTGLDPLKYLELGMNPEWLRYYLAAKLSGRNEDVDFNADDFMARVNADLIGK
                     FVNIASRAAGFISKRFGGRLGEVAPDGDQLLSHLRDAVPQLTTLYAERDYAKVVREVM
                     ALCDRVNAYVDQNKPWELAKQAGMEARLHDVCSCCIEAFRILAICLKPILPRLAAEVA
                     VFTMTPPECFDDALRPLGAGAQIGEYKHLMQRVDSKQLDALFEPPAPAPQALAAIKSI
                     ATESPLVPGGEALAPTIGIDDFARVDLRIARIVECQAVEGSTKLLRLTLDAGEGRTRN
                     VFSGIASAYRPEQLQGKLTVLVANLAPRKMKFGVSEGMVLAASHADEKAQPGIFVLEP
                     WPGAQPGMRVR"
     gene            16153..17886
                     /locus_tag="PKN51_06690"
     CDS             16153..17886
                     /locus_tag="PKN51_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNT84823.1"
                     /translation="MALAFSFRPRLLDALVGYDRARFVKDLAAGLTVAVVALPLAMAF
                     AIASGLKPEAGIFTAIIAGFLISSLGGSRVQIGGPAGAFIVIVYGIVERYGVANLILA
                     TALSGVLLFLMGLLRLGTLVRFIPIAVIIGFTNGIAVLIALSQVKDFLGLSIAKMPGD
                     FFGILGALWGHLHSFNPWALGVAAASLTVIVGWQRWLPALGPRVQFSGQLSVVPGSIV
                     ALVLATAVVGLFGLPVDTIGSRFGGIPGALPAFAWPAFSWESARFLLMPTITLALLGA
                     IESLLCARVADGMIGDRHDPNQELMAQGVANFITPFFGGMPATGTIARTVTNVKNGAT
                     SPVAGMTHALVLLVVILVAAPLASSIPLACLAAILMHVAWNMGEWREFARLKSYRMPY
                     RVTLVAVFLLTVIFDLTVAVEFGILAACVVFIYRISSLSRGERLTAADQPALAGHDAA
                     LQVWRLYGALFFGATKLVEALEDALPARVLVLDLKNVIYVDSTGAEALENLLQTCRRQ
                     GVRLVVSGLMAQPLDIARRTGLLERLQAQSDQDLQPDVASAIDTAVAGLAPVDAPATA
                     QGDAEPGPART"
     gene            complement(17883..18413)
                     /locus_tag="PKN51_06695"
     CDS             complement(17883..18413)
                     /locus_tag="PKN51_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNT84824.1"
                     /translation="MSIVKPDFSHPRARALGAVLGAALALAGCDPQAIAELEEGVATE
                     ADVVQRFGQPERVWPETGGARTYEYNRQPEGLRNYMITIGPDGKMSALRQVLTPDNFR
                     RVQPGMGVEEVRRLLGKPARQVPYELRREIVWSWKFLEPPSEKKAFNVVFSPDYRVLR
                     TEVGPDPDGPDMRGGG"
     gene            18507..18728
                     /locus_tag="PKN51_06700"
     CDS             18507..18728
                     /locus_tag="PKN51_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HNT84825.1"
                     /translation="MSLARLFQRPHYQSDATDFIDELKRARPELDLQQRQGRALLWDK
                     TVNRELQDEFRAGRVAQQAYVYYAYSDSQ"
     gene            18786..19637
                     /locus_tag="PKN51_06705"
     CDS             18786..19637
                     /locus_tag="PKN51_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNT84826.1"
                     /translation="MQAAETASLLPEAPEQAPLPEVVDHVAVARLYGEPLFALPQDLY
                     IPPDALEVFLEAFEGPLDLLLYLIRKQNFNILDIPMAAVTRQYLSYVDEVRSRNLELA
                     AEYLLMAAMLIEIKSRMLLPPKKTAEGQEPEDPRAELVRRLLEYEQMKLAAHRLAELP
                     QYGRDFLRAQVYVEQALKPRFPDVELVELQQAWADILKRARLVQHHKISREELSVREH
                     MSIVLKKLQGRRFALFEDLFDPSRGVPVLVVTLIALLELAKESLVEVTQAEAYAPIYV
                     RLAFTPA"
     gene            complement(19694..21004)
                     /locus_tag="PKN51_06710"
     CDS             complement(19694..21004)
                     /locus_tag="PKN51_06710"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNT84827.1"
                     /translation="MKILIEQGRIIDPASGRDETGDIAIAAGRILSIGRAPADFTPAR
                     RVNARGCWVLPGLVDLAARLREPGHEHARMLESEMAAAVAGGVTSLVCPPDTDPVLDE
                     PGLVEMLKTRAERLHQARVLPLGALTRGLKGEVLTEMGLLTDAGCVAFGQAEAPIVNT
                     QVLQRALQYASSFGYAIWLRPLDPWLGGGVAASGPLAMRMGLAGVPVAAETVALHTVF
                     ELLRAMSGGATAPRVHLCRLSSAAGVALLRAAKAEGLRVTADVSIHSLHLSDEDLGYF
                     DSRARLNPPLRQPRDREALRAALADGTLDALVSDHTPVEADAKQLPFAEAEPGATGLE
                     LLLPLALQWGQAPDGPGIVRALACVTQGPARVLGAALGTLQASCGRLVEGGVADVCVF
                     DPEARWAVDAAALRSQGKHTPFGGLQLTGRVRCTLVAGQLAHSA"
     gene            complement(21106..>21932)
                     /locus_tag="PKN51_06715"
     CDS             complement(21106..>21932)
                     /locus_tag="PKN51_06715"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNT84828.1"
                     /translation="VKKVPLLRGKSVFNLFFENSTRTRTTFDIAATRLSADVYTLDIA
                     RSSTNKGESLLDTVANLSAMAADMFVVRHSESGAPYLLAQHVAPHVHVINAGDGRHAH
                     PTQGLLDMFTIRHYKKDFSNLVVAIVGDVLHSRVARSDIHALTTLGAAEVRVVGPRTL
                     VPGDLAPLGVRVCHTLQEGIQGADVIIMLRLQNERMSGALLPSSQEFFKSFGLTPDKL
                     HWAKPDAIVMHPGPINRGVEIDSRVVDGGQSVILPQVTFGIAVRMAVMSIVAGNEA"
BASE COUNT         3193 a         7812 c         7668 g         3259 t
ORIGIN      
        1 gttcatgcgg cattcccaag ggttgcaagc ggtcaactgc ggtttctagg ttcaacgccc
       61 gagccgcccg cgccgccgac cggcgccgcc ccgctaccat gctgacctga tgcccccgca
      121 gctcgcccgc cacctgccgt ttctgcgctg gccgcgcccg accgcgcgcc tgctgcaagg
      181 ggaagccatg gccggcctga cggtggggct gatggtgatc ccgcagggcg tggcctacgc
      241 ccagctggcg ggcatgccgc tgatcaccgg catctacgcc tccatgctgc cggcgctggt
      301 ggcggtgctg ttttcggcct cggcgcggct gtcggtgggg ccgacggcgc tgaccagcct
      361 gatgatcgcc gccgccctga cgccgctggc cacgcccggc agcgccgaat gggtggcgct
      421 ggcggtgtgg ctggcgctgc tgtcgggcct gctgcaggtg gcgctgggcc tggtgcgctt
      481 cggctggctg ctgaacctgg tcaacgcgcc ggtgctgatg gccttcacgc aggcggcggc
      541 ggtgctgatc atcggctcgc agctgccggc actgctgggt tttcgcgccg gctgggccga
      601 catgctgcgc cagccggcct tcgatccgct ggccagcgcc ttcggcctgg gcgcgctggc
      661 gctgctgctg ctggggcggc gctggaaacc gaccttccct ggcgtgctgg tgatcgtggt
      721 ggcggcggcc gcgctgagct gggcgctcgg cttcgaagcc ggcggcggcg ccgtggtggg
      781 cgcgctgccg caggggctgc cggcgcccta cctgccgccc tggcccggct ggcaactgct
      841 gggccagttg ctgctgccga ccctggtggt gacgctggtg agctttctgg aaaccgccgc
      901 cagcgccaag gtcgacagcc agcgcaaggg cgagcgctgg gaccaggacc aggacctgat
      961 cggtcagggt ctggccaaga tcgcctccgg cctgtgcggc gcctttgcca ccagctcgtc
     1021 gttttcgcgt tcggcgctca acctgtacgc tggcgcacag accggctggg ccacggtggt
     1081 gtcggtggcg gtggtgctgc tggcgctgct gctgttcacg ccggtgctgc gcttcgtgcc
     1141 gcaggcggtg ctggcggcca tcgtggtggc ggcggtgctg gggctgatca agccgcgcga
     1201 gttcctgcgc ctgtggcggg tgtcgcgggt ggagacggtg atcgccgcgc tgaccttcgc
     1261 ggtcacgctg gcggcggcgc cggcgctgta ctggggcgtg ctgacggggg tgctgatggc
     1321 gctgtcgctg ttcctctacc agcgcctgca cccgcgcatc atcgaagtcg gtctgcacgc
     1381 cgacggcagc ctgcgcgacc gtcacctgtg gcagctggcg ccgctggcgc cgcgcacgct
     1441 ggcgctgcgg ctggacgcgg cgctggactt cgcctcggcc gggcagttcg agcgccacat
     1501 gaccgaatac ctggccgagc acccgacgac acggcatgtc tgcctgttcg cccagcccat
     1561 caaccgcatc gacgccaccg gcgccgaggt gttcgcgcgc gtgctgggcc agttgcacga
     1621 gcggcgtgtg acgctgctca tcgtcggcct caagctgccg gccgaacggg tgctgcgcgc
     1681 ggccggcgcg ctgggagagt ggccgctgct gcagatggtc cgcaccgacg ccgaggcgct
     1741 ggccgcgctg gcgcgcctgg aggagccccc ggccgacatc gccgccgccg ccatctgagg
     1801 cttgagccct tggcttcgga atcctgaact cggaaccagg ccgaatgtgc ctccggcgca
     1861 ggcacagcaa gcgccagcag ctattgattc agtagcgctt tcaacccagc ccgagcgccg
     1921 ccacgccggc ggcgatgcac agcgcgccgg ccacgcgcac cccccggtcg ccctcgccca
     1981 acagctggcc gccgatcagg gcggcgaaca gcatcgacac ctcgcgcgcc ggcgccacca
     2041 gcgacagcgg cgcctgctgc atggcgaaca gcaccagcac gtaggccacc gggctgcaca
     2101 gcgccaccac cagcgcgccg cgccactggc ggcgccacag ctgcgcggtg gcggcgcggt
     2161 cgcgcagcgc caccggcagc agcaggccga tgcgcaccca gttgcccatg tagtccagca
     2221 gaatcggcga cagcgccagc cagcgcacgg cgtagccgtc gaccacggtg tagctggcga
     2281 tccagacgcc ggtcagcagg ccgtagcgca ggccctggcg cacgcgcgca cgtgcctgcg
     2341 ggtcgtcgcg ccggcgcagc aggcgcgggc caccggcgat caggaacacc cccagcacca
     2401 cggcgccgat gccggccgcc gccagcgggc tcaggcgctc gcccagcagg gtgacggcca
     2461 ccagcgacga cagcagcggc cccgagccgc gcgccagcgg gtagaccacg gtcaggtcgg
     2521 ccacgcgata gccgcgcagc agggtgacgt agtacagcac atgcagcagg ccgctggcgg
     2581 ccacgcaggc ccaggcggcg gcgtcccagt gcggcagcgc ctgccagccc agccacaagc
     2641 ccagcggcgc ccaggccagc gccatcagca ccgaggtgaa gaaggcaaag cgggcgtcgc
     2701 cgccgctttg cttggcgacg atgttccagc ccgcgtgcac cagaccggcc agcagcacca
     2761 gcgccagcgc ctgggccgac atgagggtgg cggccgctca ggcgtggctg gcgatgcagg
     2821 cggtggccat cagctcttcc agcagggcca tcgacatccg gcccgacacc gcgtaggggt
     2881 cgagcgtggc gcgttcggag tacttgagca gcacgctggc gttgacgcgg ccgacgtcga
     2941 ccaggcccat ggtccagccg ccgaagcggc gctccaggat ttcttcgtag tgcagcagca
     3001 ccacgtcctt gtgacgcggg tcgcgctgga tggtgccgta caggtcgctg accgcctggc
     3061 gcccgccttc catgccctgc agaaacacgc cgctgccgtg cagcaggatg ccggtgatgc
     3121 cgctttcggg gttgtggcgg cgcgccgagg ccaggatgct ttcgatggcc tcgcgcgagg
     3181 cgtcgacggc gcggctggcg aacaggagtc ggacaagcat ggtgctcagc ttttctgggg
     3241 aatcagggcc aggaattcgc gccgcaggtt catgtccttg aggaacacgc cgcgcatgac
     3301 ggagttgatc atcttgctgt ccatgtcctt gacgccgcgc caggccatgc agtagtgcgt
     3361 ggcctgcatg acgatggcca ggccgtcggg ccgggtcttg gactggatgt agtcggccag
     3421 ctgcaccacc gcttcttcct ggatctgcgg gcggcccatg acccactcgg ccaggcgcgc
     3481 gtacttggac aggccgatga cgttggtgtg ctcgttgggc agcacgccga tccagagctg
     3541 gccgatgacc gggcagaagt ggtggctgca ggccgagcgc acggtgatgg ggccgacgat
     3601 catcagctcg ttcaggcgct cggcgttggg gaattcggtg atgctggggg ccttgacgta
     3661 acggcccttg aacacctcgt tgaggtacat cttggccacg cgccgcgccg tgtcgcgcgt
     3721 gttgtggtcg cccgcggtgt cgatcaccag gctgtcgagc acgccctcca tgcgggtggt
     3781 gacttcgtcg agcagctgct ccagctcgcc gggccggatg cagtcggcga tgttgtcgtt
     3841 ggcgtggtag cgccgctgcg ccgcctgcag gcgctggcgg atgcgcaccg acaccggcgt
     3901 gccttcgtcc tcggcaccgg ccggggtggg gaattcgggt tgggctttca tgggcgcaac
     3961 gatggtagca gcattggaat ccccaatgaa aatgggtgtt tgcgcatgtc tggcaagcgc
     4021 cagcagctat gatttatgta gcgcgtcatg gggcatgcgg aactcggcga tcagcggcag
     4081 gtggtccgac atgcggctcc acacgcgccc acgcggcgcc gccagactga gcgggtgcag
     4141 gccgcgtgcg tagatgtggt cgagctgcgc caccgggtag cgcgaaggat aggtcagcgt
     4201 gcgtggcccc tcgaagctgt gcagcgcctc catgcgcagc agccggcgca cgcgcgtgcc
     4261 ccagtcgttg aagtcgcccg ccacgatcag cggcgcgccg cgcggcacct cgcgcgcgat
     4321 gtaggccttc agctgcgccg tctggcggat gcggctggcc ggaatgaggc cgaaatgcac
     4381 cacgatggcg tgcacgctga caccggccac cagcagctcg gcgtgcagca ggccgcgctg
     4441 ctcgaagcgg tggtcggaca tgtcctcgtg ccgctcgtga atgatgggcc agcgcgacag
     4501 cagggcgttg ccgtgctcgc cgtggcgcgt caccgcgttg gtgcggtaga ccgcgtcgta
     4561 accctcgggc gccagaaact cggcctgcgg cagctcgggc cagccggtga agtagctggc
     4621 ttccttgcgg ttgatggcgc gcacctcctg caggcagacg atgtcggcgt ccagcgtctc
     4681 gatggccagc gccaggttgt ggatctccag ccggcgcgcc ggccccaggc cctgcacgcc
     4741 cttgtggatg ttgtaggtgg ccacgcgcag cagctggtgg gcgccgccga aatgcacctc
     4801 cctcatgccg gcgctccggc aaagcgcggc agcagcaggc aggcctcggc gttggtgggc
     4861 gagaagcagc ggtcggccgc ggcgcgccag ggcagccatt cgaaggcgtc gtgctcgcgc
     4921 gggttcagcc gcaccggcgc gcgggccggc aggcacaggc cgaacacatg ttccaggttg
     4981 tgcgtcacgc cgggagcgta gcggtggcgc cagcgcgggt agatttcgta gcggttgcgg
     5041 atgccccagt cgcgcagcag gcagccgggg gcacgcgcgt cgatgccggt ttcctcgccc
     5101 acctcgcgca cggcggtctg ggtcagcgcc tcgccggggt cgtccttgct gccggtgacg
     5161 gactgccaga actcgccctg ccggacgtcg gcccggcgga tcagcagcac ctccagcgcc
     5221 ggcgtgtgga tcacgaccag caccgattcc gggatcttga agcggcaccg gccgacgcag
     5281 gagtgcgcgg acatgggttc gccctgggcg ccgcggctgc cgttcagcgc cactgccgca
     5341 gcgcgcgctc caccaccggc cgggcgcggc ggtacatctg ccagcccgac caggcgcgca
     5401 gcgccagctg ggtggccagg cgcgggcgca ccagcagcgc gcccgccagc gccgacagcg
     5461 ccagcagtcc cagcacgtag ggcgcacggc cctggcgcgc ctgctgcacg ccgcgcacgc
     5521 cgctgcgcac gcggtcgctg gcgcgaaaca gcggctgcag cacctgggtg cgggcggcga
     5581 tctgctcgcg cagctggtcg gagcgcaggc gcagctgctc gcggcgcgcc cgcaggtcgt
     5641 cggtgggcgg ccggttcatg gctgacgcag cctctgctgg tcgcgcagca gctcatcgcg
     5701 cgtggcggcg aacatctgca ggccctggcg cgcctgctgg cgcacccaca ggaccagacc
     5761 ggcgccaccg cccagaaaca aggtggtgaa cacgcccagc gccagcaggc gctggctgtc
     5821 ccacagcagc acggtcagaa acagcgccag aaagatcagc ccgaagctga gcagcaccac
     5881 cgccagcgca cccagcacgg ccatccgggc cagccgggcc agctcttcgc gcgcctcgac
     5941 ggtgaacagc tccagccgca cctgcaccag ctcgaccaca tcggcgagca gggcacgcag
     6001 gcgcgcggcg gtgccggcgt cttgggcggg ggacgtcatg gcggccgggt accggcgcac
     6061 gccgggctca gcggcggccc agcagcacgc cgatcaggat gcccagcgcg gcggcggcgc
     6121 cgatcgacga ccaggggttg tcgtgcacgt agtcgtcggt ggcggcggcg gcctggcggg
     6181 cacgcgcacg caccacggcg tcggcgtcgg ccagcttgtc gcgggcgatg cgcaggttct
     6241 cgcgcgcgcg ggcgcgcaac tcggcgatcc tggaatcggt gtgctcggcg gtggcggcca
     6301 gcaggtcctc ggtatccgac accacgcggc gcaggttgga cgccagctgg tccttggttt
     6361 cggtcatgga ctcgatgagg tcagcgctgt ggggcatgag acttctcctt gcaggcaaaa
     6421 acccatgcta acagagtgcc cgccggcgcg aaacgcccgg cggccggcac aggcgcggcg
     6481 cgctccaggt tcagacgtag tagcgcgcca gcggctcggc cacgcacacc ggcttgtcgg
     6541 cgccctcgcg ctcgatggtg ctgcgccagg ccagctgcat gccgccttcc agcggctggg
     6601 cgtccagcag cgtcacccgc gcgcgcagcc ggctgcccac cggcaccggc gccatgaagc
     6661 gcaccttgtt caggccatag ttgacgccca tgcgcacctc gcgcacctgc accacgccgc
     6721 ccaggtggcc ggccagcatc gacagcgtca ggtagccgtg cgcgatcggg tcgccgaacg
     6781 ggccggcctt ggcgcgttcg gggtcgacgt ggatccactg gtggtcgccg gtggcctggg
     6841 caaactggtc gatgtgcgcc tgctcgacgg tgaaccagtc ggtgacggcg acttcctgtc
     6901 cgacgcaggc ggccagctcg gccagggtct cgaagtgctt gatcatgggc ggcgactgta
     6961 acgcgccgcg acggccgccg ctgtcaagcc ggcgaccggt cgagccagcg gcagatgccc
     7021 tgccactgct ccaggtcgcg cgccacgcgg ccaggcgcga tgtcgaacag cgtcagcccg
     7081 cgcgccgcca ggtgcacgta gttctgcgtc ggtcgcagca ggcccagcac cggcaggccc
     7141 aggccgtcgg tgaactggcg cagctgctcg gcggcgatgg tgcgctcgtc cacccgcatg
     7201 cccaccagcg ccagctcggc ctgccgcgcg ccgcgggcgc ccagcagttc gtcgatgaat
     7261 tcgcgcgtgg cgtagatgtc gaagatgctg ggctgcagcg gcaccagcac cttgtcggcg
     7321 aggcgcagca gctcggtcag cttgctgccg tgcagaccgg ccggggtgtc cagcaccacg
     7381 tgggtggtgc cgcgcggcgg acgcaccacg aagtcgcgca gcacgtccca ggtggcgatc
     7441 ggcgacgccg cctcggggcg caggctcagc cacaggcgcg acgactgctg gcggtcgacg
     7501 tcgcccagca tcacggcgtg gccgcggctg gcccagtagc cggccacatg gctggccagc
     7561 gtggacttgc ccacgccgcc cttgggattg gcgatcacca ccaccggcat gacgacttcc
     7621 tcctgctgca aacgcgccgg cgccaatggt agcggctgcc gacggcgcca cccgggcgcc
     7681 cgacatccat gataaaaacc ggctctggcg cttgtgtggc aagcgcaaac agctattgtt
     7741 tttgaagcaa cccggcatga ccaccccgcc cctcaccctg gccgatcagg ccgcccaggc
     7801 cgaacgcctg ctggatccgg ccgtctgggc ctacctgaac ggcggcgccg ccgacgagct
     7861 gaccctgcgc gccaacctca ccgcctggca gcgtctgccc ctgtggccgc gcgtgctgcg
     7921 cccgctgggc ggcggccaca cccgcagcgt gctggccggg cgcgagctgg cccacccgat
     7981 cctgctggcg ccggtggcgc tgcagcggct ggcccacccc gacggcgagc gcgccagcgc
     8041 cctggccgcc gccgcccagg gcgccggcta tgtgctgagc acccagtcca gcacgccgct
     8101 ggagacggtg gcccaggcgg tgctgggcga tcccggccgc gggccgctgt ggtttcagct
     8161 ctactggcag gccgaccgcg gtttcaaccg cgccctggtg cagcgcgccg aagcggccgg
     8221 cttcgaggcc atcgtgctga ccgccgatgc cccggtgcaa ggcgtgcgcg accgcgagcg
     8281 gcgcgccggt ttcgtgccgc cgccgggcgt gcgcgcggta cacctgcagg gcgccccgct
     8341 ggccccgccg gccgccgaca cctactgcgc cggcctgccg gcgcacgccc cgaactggga
     8401 cgacctcgcc tggctgcagg gccagacccg cctgccgctg tggctcaagg gcgtgctgca
     8461 cccgcaggac gcgcgccagg cgcaggccct gggcgtggcc gggctgatcg tcagcaacca
     8521 cggcgggcgc acgctggaca gcgcccccgc cagcgccgac gcgctggtgc aggtgcgcgc
     8581 ggcggtgcag gacgcgctgc cgctgatcgt cgacggcggc atccggcgcg gcaccgacgt
     8641 gctcaaggcc atcgccctgg gcgcgcgcgc ggtgctgatc ggccgcccgg ccctgcacgc
     8701 cctggccgcc ggcggcgcgc tgggcgtggc gcagatgctg cgcctgctgc gcgacgagct
     8761 ggaaatggcg atggcgctga ccggctgccg caccctggcc gacgccgggc cggccctgct
     8821 gacacccacg gcggcctgat gcgcgagtgg cgccgttgcc gatcgccgat gaccagcgac
     8881 gaatcgcctc gccgggcttc gatgacgggg tgcgcgtgcg ccatccggtg gtacgcgaac
     8941 tggcctgaac cgcctcggcg cgacggctgc gcccacttca gttccccaaa ggtattgaca
     9001 atgagaatga atcgcattta atatgcgaac cctcggccac cccgccccag cccgccatga
     9061 tcgtctgcgt ctgcaactgc atctccgaca aggaaatcgc tcgccaagcc catgccggca
     9121 tgggcttcga cgacatccag ctggaactgg gcgtggccac ctgctgcggc cgctgcgagg
     9181 acttcgcacg tgacctggtg gcccagtgca gcgccagccg gccggtggcc gcgctgcgcg
     9241 acgccggcgg agcgtccgcc gggctgatgg aggcggtgtg accctggtgt cggccctgct
     9301 tcccaccctg ggcgccctgg gcctggtgct gggcgccgcc ttctgggtgc tgcgccgtta
     9361 gttgtttcgc cgttgccgcc agcgccccgc gcgccggtgc gcggctacga cccgcctgcg
     9421 tatcatgtct tccgcctcgc tcaccccgcc ccccgccatg tcactgcccg gccgccacac
     9481 cgtcgtcgtg ggcagcgtgc tgctgatgca cggggtggcg ctgtgggcgc tgcaccaggg
     9541 gctgctgcgc ccgccggtgg agctgatcgt gccggcccag gtgctggccg aattcatcac
     9601 cccgcagccg gcgccgccgc caccggcacc gcccccgccc gaaccgtcca agccgacacc
     9661 ggcggcggtc aaaccggccc cccggccgac gccgcggccg cgcccggcgc cacagcccca
     9721 gccacagccg ctggcggtgc agcaggcggc gcccagcgcc ccggtgatgc cgatcgcccc
     9781 gcccgagcca ccggcaccgg cgcccgtggc cgccccggca cccgcaccgg cggcgccgcc
     9841 gacgccaccg gcaccgcctg caccgccggc ggtggtgcag ccgtcgtcca acgcggccta
     9901 cctgaacaac cccaaacccg cctacccggc catgagcaag cgcctgggcg aaagcggtcg
     9961 cgtggtggtg cgcgtgctga tcggccccga cggccgggcg cagcaagccc acatccagcg
    10021 ctccagcggt tacgagcggc tggaccaggt ggcgctggag accgcgcgcg accgctggcg
    10081 ctatgtgccc ggcacgcgcg gcggcgtgcc cgaggcgatg tggttcaacg tcccgatcaa
    10141 tttcgtactg gagtaaccaa gccatgaaca atgaattcgg cctgctgcac gtgtgggctc
    10201 agggcgactg ggtcacgcgc agcgtgttcg gcgcgctgct gctgatgtcg gtcgcctcct
    10261 gggtggtgat cgtgctgaag gcgctcggcc tgatgcgcac ccagcgtcag gcggcgcggg
    10321 tggaagggtt ctggcattcc agcgacatcg ccgaaggcct ggccaagctg ggcacgggcg
    10381 aagcgaatcc gtttcgcgcg ctggccatcg agggccgcga agccgccgcc caccaccgcg
    10441 ccacgcaggc gcagttgcac gattcgctgg acgtgagcga ctggatctcg cgcgcgctgc
    10501 agggcagcat cgacgccacc gcctcgcgcc tgcaggccgg gctggcggtg ctggcctcgg
    10561 tgggttcgac ggcgcccttc gtcggcctgt tcggcaccgt ctggggcatc taccacgcgc
    10621 tgatgaagat cggcgccgcc ggtcaggcca gcatcgacca ggtggccggc ccgatcggcg
    10681 aggcgctgat catgaccgcg ctgggcctgg cggtggcgat tccggcggtg ctgggctaca
    10741 acgccctggt gcgcggcaac aagaccttgc tgatgcgcct gggccgcttc gcgcacgacc
    10801 tgcacgccta ttacgtgacc ggctcgcgcg tcaacggcag cggcgacggc aaggtggtcg
    10861 ccatgaagaa ggcctgaaca ccatggccgg cttcattgct caggaccatg acgaggtgat
    10921 gagcgagatc aacatggtcc cgttcatcga cgtgatgctg gtgctgctgg tgatcttcat
    10981 catcaccgtg ccggtcatga agcacgccgt caacgtgcag ctgccgcgcg ccgccaatga
    11041 aaaggaggtg gtgcagccac agaccctgcg cctgaccgtg caggccgacg gcagctactg
    11101 gctggacgac cagaaactgg ccgacgacca gctgggcacg cgcctgcagg ccgccgccgc
    11161 gcagcagccg cagcccgacc tgcacatccg cggcgaccgc gacgtgcgct acgagcgcgt
    11221 ggcgcaggcc atggcggcgg cgcagcaggc cgggttgtcg aagatcggct tcgtcaccga
    11281 accaccccag cgcacgccat gaacggccag accgccaccc tgacgcccgc gcgccgcgcc
    11341 gccgacacca ccccggcgca ccacgcaccg gatcgcccgc ggccagccgc cgaggctgcc
    11401 gcgcccgccc cgctgccgag cgaacagctg ctgcagggcc gcaagaccat cgagatcagc
    11461 cacaacggca cgctgtaccg gctgcaggcc accaagctgg gcaagctgat cctcaccaag
    11521 taagtaggtt catcgtggga cgcgtccgac cgacgcgctt gtcggagcca gccaggccct
    11581 cacccagcca gctctctttt tttctcacgc caaaacgcgc tcggcgcttg cccatcaagc
    11641 gccaacagcc atcaaaacca gagcgctcag gcgctgccgc gatcagcgct tgcgtacccc
    11701 ggaccacggg tcctcgccgt cgggcagcgc catcagtgcg ctcagactca tcacgccgga
    11761 gtcggcgctg cgcgagtggg cgaagccgga cgcggcaaaa ccggacgacg caaagccgga
    11821 cgccttgaag tccgaggccg ccggctcggc cgccggcacc ggcgtgcgct gcgcagccag
    11881 caggcgcatg cgcaggattt cgggcggcgg aaggctggcc agcgcaccct tgaggcgcac
    11941 cagacgaacg ccggcgctct tgagcatgcg gttcttttcg ctggcgtccc gatccggccc
    12001 gcctgcggcc gggtccttga aggcgtcgac ctcgaacgcg aacagcggct tgccgtcgtc
    12061 ggcgcagacc aggaagtcga cctgctgctc cagcaggcgc tgctgggccg ccaggcggtc
    12121 gcgcgtgcgc cgcaccgcca gaaagctggc cagtcgcggc cggtacagca ccacgccgtc
    12181 gccgaaggcc tgctgcaggt agcgcagcag ttcgacctgg cgctcggtga tcgccggggt
    12241 ctggccgaag cggtcggcgc tggcgtcgta gtttcgggcg gcgcgcccgc ggcgccagga
    12301 gcggcgcacc atggcccaca gcagcatcag cgccagcagc gccacgccgg tgctgagcag
    12361 gggcagccag ggcccgctgt gctgggtcag ccagccgggc cagccgttga agatttctga
    12421 gttcatgttc cgactccctg gagcccgcag cttacgccgc gcgcgcgggg ggcgccatcc
    12481 ccccaatggt gtagggacgc gggcgcctga tgggcgccag gattccgatc aaaaacaggc
    12541 tctggcgccc aaccacaaag cgccagcggc tatttaaata gtagcgattc tgagggcgtg
    12601 cgaccggctc agctgctgcg cgagatggtg atggagggga acttgctgga atagtccttg
    12661 gcgcggtcgg cgatgcggat cgccacctgg cgcgcgatcg ccttgtagct ggtggccacc
    12721 tggccgtccg ggtcggccac cacgctgggg cggcccgagt cggcctgctc gcggatctcc
    12781 agcgccagcg gcagcgcgcc caggtactcg atgcccttgt cggccgcgta gcgcttgcca
    12841 ccgtcggcgc cgaagatgtg ctccacgtgg ccgcagttgg aacacacgtg caccgccatg
    12901 ttctccacca ggcccaggat cggcacgccg accttttcga acatcgccac ccccttgcgc
    12961 gcgtcggcca gggcgatgtc ctgcggcgtg gtgacgatga cggcgccggt cagcggcacg
    13021 cgctggctca ggctgagctg aatgtcgccg gtgccgggcg gcagatcgac gatcaggtag
    13081 tccagctcct tccagtgggt ctggcgcagc agctggtcga gcgcctgggt ggccatgggg
    13141 ccacgccaga tggtggcctg atcggggtcg accagaaagc cgatcgagat cacctgcacg
    13201 ccgtggttgg tcagcggctc catggtcttg ccgtccaggc tctcgggtcg gccgctgaga
    13261 cccatcatga tcggcatgct ggggccgtag atgtcggcgt ccaggatgcc cacgctcgcg
    13321 ccctcggcgg ccagcgccag cgccaggttg acggcggtgg tgctcttgcc cacgccgccc
    13381 ttgcccgaag ccacggcgac gatgttcttc acccccggca gcagcggcac accgcgctgc
    13441 acggcatgcg ccaccacccg ggtgacgatg ttcaccgaca cgttgccgac cccgccgagc
    13501 gtgcgcacgg ccgccaccag cgcctgccgc agggccggca tctgactgcg ggccgggtag
    13561 ccgagctcga tgtcgaagga cacgtcgcca ccgctgatct gcaggttctt cagctgtccg
    13621 gtggagacga aatcctgctg ggtgttgggg tcgagcacgg tcgccgccgc gcgccgcacg
    13681 tcttgctcga ggatggtcat ggtgttggcc cggtgtcgaa tcagccgcta gtctagtgca
    13741 gtgtttcgcg ctgtcaggta cttgtggcga gtcgccacgg ggcccgctgc gcggcgcggt
    13801 cgccgcacca tgcccgggcc cggcgcgagg ccatcgcgcg cttcttcggc tggggcgaca
    13861 cctaaaatga tgggttttgc cgcgcccatc gcgcccgctg ccatgaccgc ccgccagctc
    13921 tttgtcacca ccgccctgcc atacgccaac ggcaagttcc acatcggcca catcatggaa
    13981 tacatccagg ccgacatctg ggtgcgcttc cagcgcatga tgggcgccga ggtgaatttc
    14041 gtcggcgccg acgacgcgca tggcgcgccc atcatgattg cggccgaaaa ggccggcaag
    14101 acgcccgaac agttcgtggc cgacatcgcc gccggccgcc agcagtacct ggacggtttt
    14161 ctgatcggct tcgacaactg gcacagcacc cacagcccgg aaaacaccgc catctcgcag
    14221 gcgatctaca ccgacctgaa ggccgccggg ctgatcgaga gccgcaccat cgagcagttc
    14281 tacgacccgc agaagggcat gttcctgccc gaccgcttca tcaagggcga gtgccccaac
    14341 tgccactcca aggaccagta cggcgacaac tgcgaggtgt gcagcgccgt ctacgcaccg
    14401 accgagctga tcgagccgta ctcggcgctc accggcgccc ggcccgagct gcgcaccagc
    14461 gagcatttct tcttccgcct gtcggacccg cgctgcatcg agttcctgcg cggctggacc
    14521 gagcgcctgc aacccgaggt ggccaacaag gtccgcgagt ggatcgagcc gggcgcggac
    14581 ggccagccga agatgggcga ctgggacatc tcgcgcgacg cgccgtactt cggcatcccg
    14641 atcccggacg ccccgggcaa gtacttctac gtctggctgg acgcccccat cggctacctg
    14701 gccagcctga agaacctgct ggacaagcgc ggccaggact gggccgccta catggccaac
    14761 ccggcgctgg agcaatacca cttcatcggc aaggacatca tcaccttcca cacgctgttc
    14821 tggcccgcca tgctcaagtt cagcggccgc aaggtgcccg acaacgtgtt cgtgcacggc
    14881 tttctgaccg tcaacaacgg cgagaagatg agcaagagcc gcggcaccgg gctggatccg
    14941 ctcaagtacc tcgaactggg catgaacccc gagtggctgc gctactacct ggccgccaag
    15001 ctgtcggggc gcaacgagga cgtcgatttc aacgccgacg acttcatggc gcgcgtcaac
    15061 gccgacctga tcggcaagtt cgtcaacatc gcctcgcgcg cggccggctt catcagcaag
    15121 cgcttcggcg gccggctggg cgaggtcgcc cccgacggcg accagctgct gagccacctg
    15181 cgcgacgccg tgccccagct caccacgctg tacgccgagc gcgactacgc caaggtggtg
    15241 cgcgaggtga tggcgctgtg cgaccgcgtc aacgcctacg tcgaccagaa caagccctgg
    15301 gagctggcca agcaggccgg catggaagcg cgcctgcacg acgtgtgcag ctgctgcatc
    15361 gaggccttcc gcatcctggc gatctgcctc aagcccatcc tgccgcggct ggcggccgag
    15421 gtggcggtgt tcaccatgac gccgcccgag tgcttcgacg acgccctgcg cccgctgggc
    15481 gcgggcgcgc agatcggcga gtacaagcac ctgatgcagc gcgtggacag caagcagctg
    15541 gatgcacttt ttgagcctcc ggcgcccgcc ccgcaagcgc tggcagctat taaatcgata
    15601 gctaccgaaa gccccctcgt gccgggcggc gaggcgctgg cgcccaccat cggcatcgac
    15661 gacttcgccc gggtcgatct gcgcatcgcc cgcatcgtcg agtgccaggc ggtcgaaggc
    15721 tcgaccaagc tgctgcgcct gacgctcgac gccggcgagg gccgcacgcg caacgtgttc
    15781 agcggcatcg ccagcgccta ccggcccgag cagttgcagg gcaagctcac cgtgctggtg
    15841 gccaacctgg cgccgcgcaa gatgaagttc ggcgtcagcg aaggcatggt gctggccgcc
    15901 agccacgccg acgaaaaggc gcagcccggc atcttcgtgc tcgagccctg gccgggcgcg
    15961 cagcccggca tgcgcgtgcg ctgaacgcgg cgccgcggcg gcgtacactg acgctgccat
    16021 ggcacgccag ccgatcctgc cgctttcgcg ccgccgccgg ttcatgatcc ggcggcatca
    16081 ccatggccca ccgggaaggc cctgaacaca ccgcttgcct gcgttgtcgc tcacgtcttc
    16141 ctggagtcga acttggccct ggctttttcc tttcgtcccc gtctgctcga tgctctggtc
    16201 ggctatgacc gtgcgcgctt cgtcaaggac ctggccgccg gcctgacggt ggcggtggtg
    16261 gcgctgccgc tggccatggc gtttgccatc gccagcggac tgaagcccga agcgggcatc
    16321 ttcaccgcca tcatcgccgg ctttctgatc tcgtcgctgg gcggcagccg ggtgcagatc
    16381 ggcgggccgg cgggcgcctt catcgtcatc gtctacggca tcgtcgagcg ctacggcgtg
    16441 gccaacctga tcctggccac ggcgctgtcg ggcgtgctgc tgttcctgat ggggctgctg
    16501 cgcctgggca cgctggtgcg cttcatcccg atcgcggtga tcatcggctt caccaacggc
    16561 atcgcggtgc tgatcgcgct gtcgcaggtg aaggattttc tcggcctgtc gattgccaaa
    16621 atgccgggcg acttcttcgg catcctgggt gcgctgtggg gccacctgca cagcttcaac
    16681 ccctgggcgc tgggcgtggc ggcggcctcg ctgacggtca tcgtcggctg gcagcgctgg
    16741 ctgccggcac tggggccgcg ggtgcagttc tcgggccagc tcagcgtggt gccgggctcc
    16801 atcgtggcgc tggtgctggc gacggcagtg gtgggtctgt tcggcctgcc ggtggacacc
    16861 atcggcagcc gctttggcgg cataccgggc gcgctgccgg ccttcgcctg gccggcgttc
    16921 agctgggagt cggcgcgctt tctgctcatg cccaccatca cgctggcgct cttgggcgcc
    16981 atcgagtcgc tgctgtgcgc gcgcgtggcc gacggcatga tcggcgaccg ccacgacccc
    17041 aaccaggagc tgatggccca gggcgtggcc aacttcatca cgccgttctt cggcggcatg
    17101 ccggccacgg gcacgattgc gcgcaccgtc accaacgtga agaacggcgc caccagcccc
    17161 gtcgcgggca tgacgcatgc gctggtgctg ctggtggtga tcctggtggc ggcgccgctg
    17221 gccagctcca ttccgctggc ctgcctggcg gccatcctca tgcatgtggc ctggaacatg
    17281 ggcgagtggc gcgagttcgc gcgcctgaag agctaccgca tgccctaccg cgtcacgctg
    17341 gtggcggtgt tcctgctgac ggtgatcttc gatctcacgg tggcggtgga gttcggcatc
    17401 ctggcggcct gcgtggtgtt catctaccgc atctccagcc tgtcgcgggg cgagcgcctg
    17461 acggccgccg atcagccggc gctggccggc cacgacgcgg cgctgcaggt ctggcgcctg
    17521 tacggggcgc tgttcttcgg cgccaccaag ctggtcgaag cgctggagga cgcactgccc
    17581 gcgcgcgtcc tggtgctgga cctgaagaac gtcatctacg tcgattcgac cggcgccgag
    17641 gcgctggaaa acctgctgca gacctgccgc cgtcagggtg tgcggctggt cgtctcgggc
    17701 ctgatggcgc agccgctgga catcgcgcgc cgcaccgggc tgctggagcg gctgcaggcg
    17761 cagtcggacc aggatctgca gcctgatgtg gccagcgcca tcgacacggc agtggcaggc
    17821 ctggcgcccg tcgatgcccc cgcgaccgcc cagggcgacg ccgagccggg ccccgcgcgg
    17881 acctaaccgc cgccccgcat gtcgggcccg tccgggtcgg ggccgacttc ggtgcgcagc
    17941 acgcggtagt cggggctgaa gacaacgttg aaggccttct tctcgctcgg cggttccagg
    18001 aacttccagc tccagacgat ttcgcgccgg agctcgtagg gcacctgccg ggccggcttg
    18061 ccgagcaggc ggcgcacctc ctcgacgccc atgccgggct gcacgcggcg gaagttgtcg
    18121 ggcgtcagca cctggcgcag cgcactcatc ttgccgtcgg ggccgatggt gatcatgtag
    18181 ttgcgcaggc cctcgggctg acggttgtat tcgtaggttc tggcaccgcc ggtctccggc
    18241 cacacccgct cgggctggcc gaagcgctgc accacgtcgg cttcggtggc cacgccctct
    18301 tccagttcgg caatggcctg cgggtcgcag cccgccagcg ccagtgcggc ccccagcacg
    18361 gcgcccagcg cccgcgcgcg cggatgggaa aagtcaggtt tcacgatgct catgcccggt
    18421 aaaatgatcg gttgacgcgt gcgtgccggt ggcccgcgcg gcgcctgtgc cagcctccgt
    18481 ggcaccgatc acgaaagctc cagtgtatgt cccttgcccg cctgtttcag cgcccgcatt
    18541 accagtcgga cgccaccgat ttcatcgatg agctcaagcg cgcccggccc gagctggatc
    18601 tgcagcagcg ccaggggcgc gccctgctgt gggacaagac ggtcaatcgc gaactgcagg
    18661 acgagttccg cgccggccgt gtggcgcagc aggcctacgt gtactacgcc tacagcgatt
    18721 cacaatgaaa agcggctgtg gcgcccgcca agtaagcgcc acaagctatc ttttcagtag
    18781 cattcatgca ggctgccgaa accgcgtcct tgctgcccga ggcgcctgag caggcgccgc
    18841 tgcccgaggt ggtggaccac gtggcggtgg cgcgcctgta cggcgagccg ctgttcgcgc
    18901 tgccgcagga tctgtacatc ccgcccgatg cgctggaagt ctttctggag gccttcgaag
    18961 gcccgctgga cctgctgctg tacctgatcc gcaagcagaa cttcaacatc ctcgacatcc
    19021 cgatggcggc ggtgacgcgc cagtacctga gctacgtcga cgaggtgcgc agccgcaacc
    19081 tggagctggc ggccgaatac ctgctgatgg ccgccatgct gatcgagatc aagtcgcgca
    19141 tgctgctgcc gcccaagaag acggccgaag gccaggagcc cgaagatccg cgcgccgagc
    19201 tggtgcgccg cctgctcgaa tacgagcaga tgaagctggc cgcccaccgg ctggccgagc
    19261 tgccgcagta cgggcgcgac ttcctgcgtg cccaggtgta tgtggagcag gcgctcaaac
    19321 cgcgctttcc cgacgtcgaa ctggtggagc tgcagcaggc ctgggccgac atcctcaagc
    19381 gcgcccggct ggtgcagcac cacaagatct cgcgcgaaga actgagcgtg cgcgagcaca
    19441 tgtcgatcgt gctcaagaag ctgcagggcc ggcgctttgc gctgttcgag gacctgttcg
    19501 accccagccg cggcgtgccg gtgctggtgg tgacgctgat cgcgctgctg gagctggcca
    19561 aggaaagcct ggtcgaggtc acccaggccg aggcctacgc gcccatctac gtgcggctgg
    19621 cgttcacgcc cgcctgagcc cgggcgcgcc cgacggcggc gcgcaacgcc cgcgtgcgcg
    19681 gcccggccca ggctcaggca ctgtgcgcca gctgccctgc caccagggtg cagcgcaccc
    19741 ggccggtcag ctgcaggccg ccaaacggcg tgtgcttgcc ctggctgcgc agcgcggccg
    19801 catcgacggc ccagcgcgct tcggggtcga acacgcagac gtcggccacg ccgccttcca
    19861 ccaggcggcc gcaactggcc tgcaaggtgc ccagcgccgc gcccagcacc cgggccggcc
    19921 cctgggtgac gcaggccagc gcgcgcacga tgccgggccc gtccggcgcc tggccccatt
    19981 gcagcgccag cggcagcagc agctccagcc cggtggcgcc gggctcggct tcggcaaacg
    20041 gcaactgctt ggcgtcggcc tcgaccgggg tgtggtcgga caccagggcg tccagggtgc
    20101 cgtcggccag cgccgcccgc agcgcttcgc ggtcacgcgg ctggcgcagc ggcgggttga
    20161 ggcgggcgcg gctgtcgaag tagcccaggt cctcgtcgct caggtgcagc gaatggatgc
    20221 tgacgtcggc cgtcacgcgc aggccctcgg ccttggcggc gcgcagcagc gccacgccgg
    20281 ccgcgctgga cagccggcac aggtgcacgc gcggcgccgt ggcgccgccc gacatggcgc
    20341 gcagcagctc gaacacggtg tgcagggcca cggtttcggc cgccaccggc acgccggcca
    20401 gccccatgcg catcgccagc ggcccactgg cggccacgcc gccgccaagc caggggtcga
    20461 gcgggcgcaa ccagatcgcg tagccgaagc tgctggcata ttgcagcgcg cgctgcagca
    20521 cctgggtgtt gacgatgggc gcctcggcct gcccgaaggc cacacagccg gcgtcggtga
    20581 gcaggcccat ttcggtcagc acctcgccct tgaggccacg cgtgagcgcg cccagcggca
    20641 gcacgcgcgc ctgatgcagg cgctcggcgc gggtcttgag catttcgacc aggccgggct
    20701 cgtccagcac cggatcggtg tcgggcgggc agaccaggct ggtgacgccg cccgccaccg
    20761 cggcggccat ttcgctttcg agcatgcgcg cgtgctcgtg gccgggctca cgcaggcgcg
    20821 ccgccagatc gaccagaccg ggcaacaccc agcagccgcg cgcattgacg cggcgggcgg
    20881 gcgtgaagtc ggccggcgcc cgaccgatgc tcaggatgcg tccggcggcg atggcgatgt
    20941 cgcccgtttc gtcacggccc gaggccggat cgatgatgcg cccttgctcg atcaggatct
    21001 tcatggttta cacatcaaaa caggctgcgg cgcttatcca gcaagcgcca gcagctatca
    21061 ttttcgaact ttttcgatgg gccaggcatg ccgcaccgcg tcggttcacg cctcgttgcc
    21121 ggccacgatg ctcatcaccg ccatgcgcac cgcgatgccg aaggtgacct gcggcaggat
    21181 cacgctctga ccaccatcga ccacccgcga atcgatctcg accccgcggt tgatcggccc
    21241 gggatgcatg acgatggcat cgggcttggc ccagtgcagc ttgtcgggcg tgaggccgaa
    21301 gctcttgaag aactcctggc tcgacggcag cagcgcgccg ctcatgcgct cgttctgcag
    21361 gcgcagcatg atgatcacgt cggcgccctg aatgccctcc tgcagcgtgt ggcagacgcg
    21421 cacgcccagg ggcgccagat cgcccggcac cagcgtgcgc gggccgacca cccgcacctc
    21481 ggcggcaccc agggtggtga gggcgtggat gtcggagcgc gccacgcgcg aatgcagcac
    21541 atcgcccacg atggccacca ccaggttgga aaaatccttc ttgtagtggc gaatggtgaa
    21601 catgtcgagc agcccctgcg tcgggtgcgc gtggcgcccg tcgccggcat tgatgacgtg
    21661 cacatgcggc gccacgtgct gggccagcag gtagggcgcg ccactttcgc tgtggcgcac
    21721 cacgaacatg tcggccgcca tggcgctcag gttggccacc gtgtcgagca gggattcgcc
    21781 cttgttggtc gacgagcgcg cgatgtccag cgtgtagacg tcggccgaca gccgggtggc
    21841 ggcgatgtcg aaggtggtgc gggtgcgggt gctgttttcg aagaacaggt tgaacacgct
    21901 cttgccgcgc agcagcggca ccttcttgac ct
//
LOCUS       DAOQTZ010000041        21667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2841_length_21667_cov_13.616509, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000041 DAOQTZ010000000
VERSION     DAOQTZ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 21667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21667
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2841_length_21667_cov_13.616509"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKN51_06720"
     CDS             <1..200
                     /locus_tag="PKN51_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HNT84829.1"
                     /translation="EAMFLGGLPKGEFLREFEPDFFFDDQSGHIAHAARHVPAGHVPE
                     GVTNATPAAETSRRRRPSKRR"
     gene            197..592
                     /locus_tag="PKN51_06725"
     CDS             197..592
                     /locus_tag="PKN51_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNT84830.1"
                     /translation="MKPSFWHLPLLLLAVWASPAPAQTAAAATTAPGAARVSKGEAQA
                     LKIFRMLDADGDGRISRQEAQVGIRLKPSLAEDFRRADLNGDGHLTEQEIRSVAERRR
                     AERLERRRKEQALAARPATEPRSGSAVRP"
     gene            complement(620..1930)
                     /locus_tag="PKN51_06730"
     CDS             complement(620..1930)
                     /locus_tag="PKN51_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNT84831.1"
                     /translation="MSDTDTLLPVSAWPYTQILMRLALGLALGLLIGLERERRGKEAG
                     LRTFGFIGLLGTMGGALGMGYGLATLALTGLLVLILNVSSLRANQGTELTTSAAMLVT
                     GMAGIMCGRGHTIAPAAVMVIATALLAWKERLADFSMGVSEGEIRSAILLAVLAIVIY
                     PALPQGTVGPWGLIEPRAAWVAVILIAGIGFFNYVLWKMYGTRGTELSGFFGGLINSN
                     FTVIELVERVREVGVAYVGTAYRGIMLAMVAMIARNATLLLILAPEALISAFSAFLLM
                     MGASALLVGVSLRSGRRSAAGVAPEIHLDMPFSLPQALKYGAIFLLLHALGALTQRQF
                     GDAGFYFVSIVGGLLSSASSVAAAASLAAQGGLPPGTAGVGAVLASFTSIVFSLSFVL
                     RSRSGALIRQLALSMLLIAAAGLVGLLAGQFMQPWVGALLSRME"
     gene            2213..4033
                     /locus_tag="PKN51_06735"
     CDS             2213..4033
                     /locus_tag="PKN51_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNT84832.1"
                     /translation="MPMPQRLAQFQQFLRQVWRLSAPYFSASDERWRARGLLTAIVVL
                     NLGAVYMLVQINEWNRVFYDALQNKDAAVFWTQLGRFTLLAMAFVVIAVYRFYLTQLL
                     QLRWRAWMTRDLMGRWLQGQAFYRMELARYAGAAVPTTGATGLPHPGQREPDNPDQRL
                     QEDVNLFTAQTVGLSMGLLNAVVTLASFVGILWGLSGAFSFTLGERTIELPGFMVWLA
                     LLYCIAGSVLTHYIGRPLIGLNFEQQKREADFRHHLVRVREYSEAIALDRGERVEQRN
                     LDTRFTHLLANYLRLIGKQKQLVGFTNLFGQAAVVFPFIVAAPRFFSGAIQLGELMQI
                     ASAFDRVQGALSWFVENYDSLASWRATTERLTSFEAAISKQNTATEPTQQAPAATDSG
                     VIEAHGLNLRLPTGASLLAATELRVAPGEHTLVQGPSGSGKSTLFRALAGIWPFAQGQ
                     VALPARTMFIPQRPYVPDGALRDALAYPEPASRYSDAQLQDALRDALLPALAARLDER
                     DNWTQKLSGGELQRLAIARVLLRQPAWVLADEATSALDVAAEQQLYARLKALTERTGG
                     ALVSIAHRPGVAAFHDRYWRFVPQTEGAAARYRIESAALA"
     gene            complement(4114..4746)
                     /locus_tag="PKN51_06740"
     CDS             complement(4114..4746)
                     /locus_tag="PKN51_06740"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNT84833.1"
                     /translation="MTAHAHRTTRRHPRSVPRRPAGTPWHRLRRAGGTALLLSVLWGG
                     AGAVQAQVTEGVDWSLSAAAAADAGVRKLGLIAGWTQAAPLWQGTQWRLRLRHEIELA
                     AWHVPRARDLVELGYSPVLRLERPLDGAHATLFLEGSIGARLLSRTRVAPQRSLSTAF
                     QFSDMLGVGVQWGTQARSTLGLRYQHVSNLGIKEPNPGMDFVQVYYTHRF"
     gene            complement(4842..5462)
                     /locus_tag="PKN51_06745"
     CDS             complement(4842..5462)
                     /locus_tag="PKN51_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNT84834.1"
                     /translation="MTDLVPLTAAVRPRGSDELAQALIHGRHTVLPRRLGLPGPDAAQ
                     TERILGAAASAPDHHTLLPWRFILVPDAARPRLAEAFAAALVQRDPQATDEQIGQARE
                     KAFRAPLLMLAVVRLRDDDADIPPHERIVSAGCAIQNLLLMAHALGFGAALTSGKALA
                     SAPLRTLFGLGEAEQALCFVSVGTVLKARPARARPLPAQYVSTLAP"
     gene            complement(5664..9287)
                     /locus_tag="PKN51_06750"
     CDS             complement(5664..9287)
                     /locus_tag="PKN51_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HNT84835.1"
                     /translation="MKRDSLPGAQHALSAQPISRDVLAEKYFKPGETGLDQLYGRVAR
                     ALASVEKPELRAEWEQKFLANLHAGAIGAGRIMSAAGTDIQATLINCFVQPVGDAIQG
                     RDADGYPGIYEALREAAETMRRGGGVGYDFSRIRPKGAFVKGTHSFASGPCSYMNVFD
                     QSCSTVESAGSRRGAQMGVLRIDHPDVLEFITAKRTPGRWNNFNVSVGVSDAFMQAVI
                     DGSDWELAHKAEPGDKVKAKGAYQRADGLWVYRKVPARELWDTIMKSAYDFAEPGILF
                     LDQIGRDNNLNYTEQIEATNPCVTADTWVMTEDGPAQVADLMGRPFNAIVDGAAHPLL
                     SQGFFATGHKPVVHLQTREGHSLRLTADHRVRRVTRKTRYVIESAWEPAGQLRPGDEI
                     MLQQHRTLQGWDGAGTEAEGYLLGLLLGDGVLKEDKAVISVWAPELRQVAQGAPAHAL
                     SGADGIIAAAEAAARTLPHRADFGGFQRAVRGRAERRMASGALRHLALSHGLTPGRKS
                     VTPSMEKTSSAFHIGALRGLFDTDGSVQGAQDKGVSVRLAQSDLGTLQAAQRMLLRLG
                     IASTIYPERRPTGVRALPDGRGGLDCYRTAAQHELVVSGDNLALFADRIGFADTDKAA
                     RLTGALAQYQRQLNRERFTATVSAVTPDGSADVYDVTVADVHAFDANGLYVHNCGEQP
                     LPPYGCCDLGPIILTNFVRHPFGAGGEPSFDFDAFEQVVAVQVRALDNVLDVTFWPLD
                     QQRAESASKRRIGVGFTGLGNALSMLKLRYDREPGRQMAAEIGRRMRDAAYRASVELA
                     KEKGPFPKFQADGYLAQGTFASRLPEDIQAAIRQHGIRNSHLLSIAPTGTVSLAFADN
                     ASNGIEPAFSWGYKRNKREHDGSTSSYMVEDHAFRLYRSLIDPGATPEQPETLPEYFV
                     NALEMTAHEHVAMMQAVQPYVDTSISKTVNIPEDYPYEDFKDLYRQAWQAGLKGLATY
                     RPNSILGAVLETTPAKKEEPVAQAPAPLPVTPYDPMRTVIEKRPAGGLPAVAEKIEYW
                     TSDGQQRLYLIVTFLPVPSADGKGTIERAIEFFMPVGQSGESQQWITATMRMLSLAAR
                     GGFLDRALSDMRKVTWDRGPVRLGTYQKADGTHVPRWHDSEVAAIGYAIENLIVRRAQ
                     AAQASLFEPDELLQAEPEASASAAVPGVMAGKKCPECGAHAMIRKDGCDYCTQCGYVG
                     SCG"
     gene            complement(9587..11119)
                     /locus_tag="PKN51_06755"
     CDS             complement(9587..11119)
                     /locus_tag="PKN51_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNT84836.1"
                     /translation="MSAEPAFRAVADLIREHARRDPAHPALIDDRQQLSYGELDALMD
                     RVAAALQRDGVSPGEAIVVCGLNAVPYAALYLGALRAGVVVAPLAPSSTPQALAAMVA
                     DARARVLFLDATAPAQHLPGPDRLPRVSLDGVAPGRAFADWLAPVGAQPAPVALVPEA
                     PFNIIYSSGTTGTPKGIVQSHGMRWMHVRRGAVYDYGPHGVTLLSTPLYSNTTLVVFF
                     PTLGYGGAVVLMPKFDALRYLQLAQQHRVTHTMLVPVQYQRIMALPQFGAFDLSAFRV
                     KFCTSAPFSAALKADVLARWPGGLTEFYGMTEGGGTCILDAHLHPDKLHTVGRPAQGH
                     DIRLIDEQGREVASGEAGEVVGHSPGMMTGYHNQPDKTREAEWFDAAGKRFIRTGDVG
                     RFDAEGFLTLLDRRKDMIISGGFNIYPSDLEAQLRQHPAVADVAVVGVPSERWGETPV
                     AFVVPQPGQAVEAAALMHWFNQRAGKTQRLADLHLLPELPRSPIGKVLKRDLRDGYGR
                     TG"
     gene            complement(11158..12117)
                     /locus_tag="PKN51_06760"
     CDS             complement(11158..12117)
                     /locus_tag="PKN51_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT84837.1"
                     /translation="MKRRLLTLVVACMGAWYATAVQAQADYPNQSITMIVPYAPGGTT
                     DVLGRALAASLSRQLKQPVVVENKSGAGGTLGVVDLMRAKPDGYRLTLVPVSVFRQPY
                     VQKTPYDPIRDLTYIASFTAYDFILAVAADSPFKSVKDVVEHARGRKDAVDYGTPGKF
                     TGNQVAMALLAKAAGVSFVHVPFKGDSESTAALLGGHVKTIVSTNGVLSFMESGKVRA
                     LAIAADQRPPAFAGVPTFREAGYDVVVPSPLGLAGPKGLPSAIVQKLDAAVKAALDDP
                     DMQRVIKTYGVRTDYRDHQAYAEFARRNFEAEKSIVQSLGLND"
     gene            complement(12253..13452)
                     /locus_tag="PKN51_06765"
     CDS             complement(12253..13452)
                     /locus_tag="PKN51_06765"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNT84838.1"
                     /translation="MRQAVIVSTARTPLAKSHRGEFNITPGPQLGAHAVRAAVERAGI
                     EPGLIEDLIWGCGYPEGTMGRNIGRQTALRAGLPVTVGGTVINRFCSSGLQAIAVAAG
                     RIVMDGVPAMVAGGAESISLLNPAGRGGTAHLDPWLVEHKPQLFMEMIDTADVVAARY
                     GISREDQDAFALQSQQRTAAAQAGGVFDNEIVPCKTTMAEKNKDTGEVTHKEVTVTMD
                     NCNRPTTTLAGLAGLEPVKGAGKFVTAGNASQLSDGAAACVLMEAQEAERRGLQPLGA
                     FRGLAIAGCEPDEMGIGPVFAVPRLLERAGLTVQDIDLWELNEAFASQALYCQRRLAI
                     PMERLNVNGGAISIGHPFGMTGARLAGHILLEGQRRKARGERVKWGVVTMCIGGGMGA
                     AGLFEIF"
     gene            complement(13687..15807)
                     /locus_tag="PKN51_06770"
     CDS             complement(13687..15807)
                     /locus_tag="PKN51_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNT84839.1"
                     /translation="MNASQGAGPVQTRLDGDVLVVTIDNPPVNALGQAVRAGLLAAVE
                     ELERTAQARAMLIVGAGKAFIAGADIREFGKPPQPPSLPETINRMEACDKLVVCAIHG
                     PALGGGLEVAMATHYRLALPAARLGLPEVALGLLPGAGGTQRSPRLMGALPAAELMLS
                     GKHLSAKAALAAGLVDRLVEGDDPLAAGLAYVRELLAAGTAPRRTSALAVPGDKAQTL
                     AALDQLAQDTAKKTRGLFSPARIIDCVRVAVQTPFAEGAKRERELFVECLNSPQRAGL
                     VHAFFAERETAKIPEAKAAAPRPFAKLAIIGGGTMGAGITVSALDAGLSVVMIERDAE
                     SIARGQKNVEKVYDGLIAKGRMTAEAKAATMARYQPSTRYEDIADVDLVIEAVFEDIA
                     VKQAVFRELDRVCKSGCVLATNTSYLDIDAIAAVTSRPQDVIGLHFFSPANIMRLLEI
                     VVPARVAPDVVVTAFELAKRMKKVPVRAGVCDGFIGNRILAVYREAANHVLEDGASPY
                     EIDAAVRAFGYPMGPFQVSDLAGGDIGWATRKRKAATRDPRARYVEIADRIAERGWYG
                     QKTGRGWYLYPEGARVGQQDPEVLAIVDAERQKKGIAPRAFTHEEIMRRYMAAMVNEG
                     AKVLEEGIALRPLDIDVTFLFGYGFPRHRGGPMLWADMQGLPKVLADIEEFAREDAQF
                     WQPAALLKRLVAEGKNFASLNQRG"
     gene            15920..16840
                     /locus_tag="PKN51_06775"
     CDS             15920..16840
                     /locus_tag="PKN51_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNT84840.1"
                     /translation="MDLQSLTLLVEIIDSGNLSQAARKLRMTRANVSYHLTQLEQSVG
                     AQLLRRSTRHVEPTEIGLRLYEHGRAIQNEMLAARETVTRLGQGLMGRVGISMPSGYG
                     QMVMSHWLIEFKRRYPGIVLDVVFENRVDVLRDDVDIAVRVLPEPPPHLVARELDEVR
                     YLACASSAFARTHGLPTRLEQLTTLPLITASVVGRQLSLRGYRQDERHEVLLAPTLIS
                     DHYPFLRQGILAGLGLGLVPDYVVQDVVASGAVVTALDDFRLSIFGTRMYLLHLPGRH
                     QTRAVRTCIDFLLARAAGQSAPPTPGEPPA"
     gene            complement(17002..17877)
                     /gene="htpX"
                     /locus_tag="PKN51_06780"
     CDS             complement(17002..17877)
                     /gene="htpX"
                     /locus_tag="PKN51_06780"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNT84841.1"
                     /translation="MKRILLFVLTNLAVMVVVGIVTSLLGVGRYTGTNTGQLMVFSLV
                     VGFSGAIVSLLMSKSMAKMSMGVQVIAQPRNADEAWIVDTVRKFADQAGIGMPEVGIY
                     EGEPNAFATGAFKNSALVAVSTGLLRGMTREEVEAVIGHEVAHVANGDMVTMALIQGV
                     MNTFVVFASRVIGSLVDGFLRRDSESSGPGIGYYVTTIVLDILFGVLAGIIVAWFSRQ
                     REFRADRGAAQMMGQPNSMINALARLGGMHAEGAEMPKNLAAMGIAGGIGKLFSTHPP
                     MQERIAALRAFQAQP"
     gene            18086..19240
                     /gene="argE"
                     /locus_tag="PKN51_06785"
     CDS             18086..19240
                     /gene="argE"
                     /locus_tag="PKN51_06785"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HNT84842.1"
                     /translation="MNTLDWLGRLVAIDTTSRNSNLGLIETVRDALTAQGLKPWLAAN
                     ADGSKANLFCTLPAADGQTQGGIILSGHTDVVPVDGQDWASDPFQLTDRGDGRLYARG
                     SCDMKGFIASSLALVPEALAAPRRKPLHLALSYDEEVGCVGAPVMLAELNRRGIRADG
                     CVVGEPTSMKPVVAHKGGYFFRCTVHGKAAHSSLTPQGCNAIEHAARLICHIRDLADA
                     WRAQGPFDAAFDVPHSTITTNLIAGGIAINTVPDACSFSYDFRHLPGHDASGIQAQVQ
                     RYVDDELLPRMRREYAGARVDIECGAAVPTLDASEQAAITQLVRALTQDQGVRKVAYG
                     TEAGQFAQAGIPAILCGPGSIEQAHKPDEYVEHAQLALCDRFLRQVLATL"
     gene            19257..20828
                     /locus_tag="PKN51_06790"
     CDS             19257..20828
                     /locus_tag="PKN51_06790"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT84843.1"
                     /translation="MSPTTPAACARLIAQLQHDLRASGAADVQCLETHISWLLLAGEH
                     AYKIKKPLTLDFLDFGTLAQRRAACEEELRINRRSAPELYLEVLPITGTPAAPRLGGD
                     AAQAIEWAVHMRRFPPGALLAERAARGALGPVQIDALARAVATFQAQAAQAPADSPWG
                     HAADLARLAHDNFAPLAALGAGTALAGTLQRLAHWSETEGQRLAPLMAARRAAGRVRE
                     GHGDLHLGNLLWLEGRARLFDAIEFDPALRWIDVACDIAFVFMDLHAHGLAALAWRFL
                     NAWLEHSGDFGAVARLPYDAVYRALVRAKVAALRAGQGQGGVARTAAEAEARRYVQLA
                     AQLAAPRRPWLALTVGVSGSGKSSQTQTLIERRALVRLRADVERKRLFGLAPEAASAG
                     VPGGIYGEATSDRLFSHLRDQARALLQAGQPVLVDATFIRRARRAPFIALAEELGLPW
                     RLLAFDAPEQVLRQRVRERAAQGGDASEADEAVLHRQLAQRQPLTANEASHALTIDAR
                     QPVDWAAWDSALPGA"
     gene            complement(21001..>21667)
                     /locus_tag="PKN51_06795"
     CDS             complement(21001..>21667)
                     /locus_tag="PKN51_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HNT84844.1"
                     /translation="PVRFVGQQALPEGQAYEAFIRARRQVPTRDNAHDLFNGLVWLRL
                     PRTKGRLNALQADEIARAGVGARRGPVRDACTVFDENAALLHAPDALWQALVERRWAE
                     LFGPLRPLWGQASLLVFGHAALEKLLSPYKSITAHVWRVALPFDAAGELAALDAWLAA
                     DLTPDKLARKPFVPLPLLGVPGWWPDNAQPAFYDDAEVFRPRGRARAAGGSERFFTMT
                     NDR"
BASE COUNT         3258 a         7693 c         7464 g         3252 t
ORIGIN      
        1 acgaagccat gttcctgggc ggcctgccca agggcgagtt cctgcgcgag ttcgaacccg
       61 acttcttctt cgacgaccag agcggccaca tcgcccacgc cgcccgccac gtgccggccg
      121 gtcacgtgcc cgaaggtgtg accaatgcca cgccggcggc ggaaacttcg cgccgccgcc
      181 gcccctccaa gcgccgatga aaccgtcctt ctggcacctg cccctcctgc tgctggccgt
      241 ctgggcctcg cccgcgccgg cccagaccgc tgccgcggcg accaccgcgc ccggcgccgc
      301 ccgcgtcagc aagggcgagg cgcaggcact gaagatcttc cgcatgctcg acgccgacgg
      361 cgacggccgc atctcgcgtc aggaggcgca ggtaggcatt cgcctcaagc cttcgctggc
      421 cgaggacttc cggcgtgccg acctgaacgg cgacggccac ctgaccgagc aggagatccg
      481 cagcgtcgcc gaacgccgcc gcgccgagcg actggagcgc cggcgcaagg agcaggcgct
      541 ggccgcacgc cccgccaccg agccgcgcag cggcagcgcc gtacgcccct gagcctttca
      601 tcggccgctg gcatgacggt cattccatgc ggctgagcag ggcgccgacc caaggctgca
      661 tgaactgccc cgccagcagg cccaccagac cggcggcggc gatcagcagc atcgacagcg
      721 ccagctggcg gatcagcgcg ccgctgcgcg agcgcagcac gaacgacaga ctgaacacga
      781 tgctggtgaa cgaggccagc accgcgccca ccccggccgt gcccggcgga aggccgccct
      841 gcgccgccag cgaggcggcc gccgccaccg agctggcgct ggacagcagg ccaccgacga
      901 tgctgacgaa gtaaaacccg gcgtcgccga actgccgctg cgtcagcgcg cccagcgcgt
      961 gcagcagcag aaagatcgcc ccgtacttga gcgcctgcgg cagcgaaaac ggcatgtcca
     1021 ggtgaatttc gggcgccacc ccggcggccg aacggcgacc gctgcgcagg ctgaccccca
     1081 ccagcagcgc gctggccccc atcatcagca ggaaggcgga gaaggcgctg atgagcgcct
     1141 cgggcgccag gatcagcagc agcgtggcgt tgcgcgcgat catcgccacc atcgccagca
     1201 tgatgccgcg gtaggccgtg ccgacgtagg ccacgcccac ctcgcgcacc cgttcgacca
     1261 gctcgatgac ggtgaagttg ctgttgatca ggccaccaaa aaagcccgac agctcggtgc
     1321 cgcgcgtgcc gtacatcttc cacagcacgt agttgaagaa gccgatgccg gcgatcagga
     1381 tcaccgccac ccaggcggcg cgcggctcga tcagccccca gggaccgacg gtgccctgcg
     1441 gcagcgccgg atagatcacg atggccagca ccgccagcag gatggccgag cggatttcgc
     1501 cctccgacac gcccatggag aagtcggcca ggcgctcttt ccaggccagc agcgcagtgg
     1561 caatcaccat caccgccgcc ggcgcgatgg tgtggccacg cccgcacata atgccggcca
     1621 tgccggtgac cagcatggcg gccgaggtgg tcagctcggt gccctggttg gcacgcaacg
     1681 acgagacgtt gaggatcagc accagcagac cggtcagtgc cagcgtggcc agcccgtagc
     1741 ccatgcccag cgccccgccc atggtgccca gcaggccgat gaagccgaag gtgcgcaggc
     1801 cggcctcctt gccgcggcgc tcgcgctcca gcccgatcag caaccccagc gccagcccca
     1861 gcgccagccg catgaggatc tgggtgtagg gccaggcact gacgggcagc agggtatcgg
     1921 tgtcggacat ccgggcgatc ttgccctatg gatggggcct gcgcaatatg acaaagtcgc
     1981 gcgcgtgggc ccgccgcgac gggccttcag ccccgcttat ggattcggcc tttggatgtc
     2041 ttgaataccg ttcgggctga gcttgtcgaa gccgttggac aggctcaggg cgaatgggtc
     2101 ggatttcaag accggaagca agtcaacgcc ccaagccatg gttagcatgg ctccctgctc
     2161 cctgctccct gctccctgct ccctgctccc tgctccctgc tccctgccca ccatgcccat
     2221 gccccaacgc cttgcccagt tccagcagtt tctgcgccag gtctggcgcc tgtcggcacc
     2281 ctacttcagc gcctcggacg aacgctggcg cgcccgcggg ctgttgacgg ccatcgtcgt
     2341 gctcaacctg ggcgcggtgt acatgctggt gcagatcaac gaatggaacc gcgtgttcta
     2401 cgacgcgctg cagaacaagg acgccgccgt gttctggacc cagctggggc gcttcacgct
     2461 gctggcgatg gctttcgtgg tgattgccgt ctatcgcttc tacctgacgc agctgctgca
     2521 actgcgctgg cgcgcctgga tgacgcgcga cctgatgggc cgctggctgc agggccaggc
     2581 cttctaccgc atggagctgg cgcgctacgc cggcgccgcc gtgcccacca ccggcgccac
     2641 cggcctgccc caccccggcc agcgcgagcc cgacaacccc gaccagcgcc tgcaggaaga
     2701 cgtgaacctg ttcaccgcgc agaccgtggg gctgtcgatg ggcctgctca acgccgtggt
     2761 cacgctggcc agcttcgtcg gcattctgtg ggggctgtcg ggcgcgttct cgttcacgct
     2821 gggtgaacga acgatcgagc tgccaggctt catggtgtgg ctggcgctgc tgtactgcat
     2881 cgccggcagc gtgctgacgc actacatcgg ccgcccgctg atcgggctga acttcgagca
     2941 gcaaaagcgc gaggccgact ttcggcatca cctggtgcgg gtgcgtgaat acagcgaggc
     3001 catcgccctg gaccgcggcg agcgggtgga gcagcgcaac ctggacacgc gcttcaccca
     3061 cctgctggcc aactacctgc gcctgatcgg caagcagaag cagctggttg gcttcaccaa
     3121 cctgttcggt caggcggcgg tggtgttccc gttcatcgtc gccgcgccgc gctttttttc
     3181 cggcgccatc cagctgggcg agctgatgca gatcgccagc gccttcgacc gcgtgcaggg
     3241 cgcgctgtcc tggttcgtcg aaaactacga cagcctggcc agctggcgcg ccaccaccga
     3301 gcgcctgacc agcttcgaag ccgcgatttc aaagcaaaat acggccacag agcccaccca
     3361 gcaagcgcca gcagctactg attcaggagt tatcgaagcg catggcctga acctgcgcct
     3421 gcccaccgga gccagcctgc tggccgcaac cgagctgcgc gtggcgccgg gtgagcacac
     3481 gttggtgcag gggccgtcgg gcagcggcaa gtccacgctg tttcgtgccc tggccggcat
     3541 ctggcccttc gcgcaagggc aggtggcgct gccggcgcgc accatgttca tcccgcagcg
     3601 gccctacgtc ccggacggcg cactgcgcga cgcgctggcc taccccgaac cggccagccg
     3661 ctacagcgac gcgcagctgc aggacgcgct gcgcgacgcc ctgctgccgg cgctggcggc
     3721 gcggctggat gagcgtgaca actggacgca gaagctcagc ggtggcgagt tgcagcgcct
     3781 ggccattgcc cgcgtgctgc tgcggcaacc ggcctgggtg ctggccgacg aagccaccag
     3841 cgcgctcgac gtggcggccg agcagcagct ctatgcgcgc ctgaaggcgc tcaccgaacg
     3901 gaccggcggc gccctggtat ccatcgccca ccggcccggc gtggcggcct ttcacgaccg
     3961 gtactggcgc ttcgtgccgc aaaccgaagg ggccgccgca cgctaccgca tcgagtcggc
     4021 ggcgctggcc tgagccccag ggtcaggcat gggtggccag atcggccgca tcgcccgtcc
     4081 catcggcgcc accagccaca cccaatggga gcatcagaag cggtgcgtgt agtacacctg
     4141 cacgaagtcc atgccggggt tgggctcctt gatgcccagg ttggacacat gctggtagcg
     4201 cagccccagc gtggagcgcg cctgcgtgcc ccattgcacg ccgacgccca gcatgtcgga
     4261 gaactggaac gccgtgctga gcgaacgctg gggcgccacc cgggtgcgcg acagcaggcg
     4321 ggcaccgatc gagccttcga ggaacagcgt cgcatgtgcg ccgtcgagcg ggcgctccag
     4381 ccgcagcacc ggcgaatagc ccagctccac caggtcgcgc gcccgcggca catgccaggc
     4441 cgccagctcg atctcgtggc gcagccgcag gcgccactgg gtgccctgcc acaacggcgc
     4501 cgcctgggtc cagccggcaa tcaggccgag cttgcgcaca ccggcatcgg ccgctgcggc
     4561 ggccgacagg ctccagtcca ccccttcggt cacctgggcc tgcacggcgc cggcgccacc
     4621 ccacaggacg gacagcagca gcgccgtgcc gccagcccgg cgcaggcggt gccatggcgt
     4681 gccggcgggc cggcgcggca cgctgcgcgg gtggcggcgg gtggtacggt gggcgtgtgc
     4741 cgtcatggcg gcatcgtaca caagccgcag cctgggcgaa accgcctgta ccgcgcgcgc
     4801 aacaagcgcc gccagctatc aaaaacgcaa cgtacgaagc ctcaaggcgc cagcgtgctg
     4861 acgtactgcg ccggcagcgg tcgcgcccgc gccggcctgg ccttgagcac cgtgcccacg
     4921 ctgacgaagc acagcgcctg ctccgcctcg cccagcccga acagcgtgcg cagcggtgcc
     4981 gacgccagcg ccttgccgct ggtcagcgcc gccccgaagc ccagcgcgtg cgccatcagc
     5041 agcaggttct gaatcgcgca gccggccgag acgatgcgtt cgtgcggcgg aatgtcggca
     5101 tcgtcgtctc gcaggcgcac caccgccagc atcagcaagg gagcgcgaaa cgccttctcg
     5161 cgcgcctgcc cgatctgctc gtccgtggcc tgggggtcgc gctgcaccag ggccgccgcg
     5221 aacgcctcgg ccaggcgcgg gcgcgccgcg tccggcacca ggatgaagcg ccagggcagc
     5281 agcgtgtgat ggtcgggcgc gctggcggcg gcgcccagga tgcgctcggt ctgcgctgca
     5341 tccgggccgg gcaggcccag gcggcgcggc agcaccgtgt ggcgaccgtg gatcagcgcc
     5401 tgtgccagct cgtcgctgcc gcgcgggcgt acggcggcgg tcaggggaac gaggtcggtc
     5461 atgcccggca gcctggcctg acgctcgaat cgccgggtct gcacaaggtc accggtgggg
     5521 gcacagggtg taccaggatc atcgtcatac gcaccgtcaa gcccgggcat tcggtccgtc
     5581 acgacgtccc tgccacgaac ccgttccggc cccgtcgccg gccgcgaacc atgtccgcac
     5641 cctacaccgg cacgcagcag ccatcagccg cagctgccca cgtagccgca ctgcgtgcag
     5701 tagtcgcagc cgtccttgcg gatcatggcg tgggcgccgc attcggggca tttcttgccc
     5761 gccatcacgc ccggcacggc ggccgaggct gacgcctcgg gctcggcctg cagcaactcg
     5821 tccggctcga acagcgatgc ctgcgcggcc tgggcacggc ggacgatgag gttttcgatg
     5881 gcgtagccga tggccgccac ttcgctgtcg tgccagcgcg gcacgtgggt gccgtcggcc
     5941 ttctggtagg tgcccaggcg caccgggccg cggtcccagg tgaccttgcg catgtccgac
     6001 agcgcgcggt ccagaaaacc gccgcgcgcc gccaggctga gcatgcgcat ggtggcggtg
     6061 atccactgct gcgactcgcc gctctgcccc accggcatga agaattcgat ggcgcgttcg
     6121 atggtgccct tgccgtcggc gctgggcacc ggcaggaagg tgacgatcag gtacaggcgc
     6181 tgctggccat cgctggtcca gtattcgatc ttttcggcca ccgccggcag gccaccggcc
     6241 gggcgctttt cgatcaccgt gcgcatgggg tcgtaggggg tgacgggcag cggcgccggc
     6301 gcctgggcca ccggctcctc cttcttggcc ggcgtggttt cgagcaccgc acccaggatg
     6361 ctgttggggc ggtaggtggc cagccccttc aacccggcct gccaggcctg gcggtacaga
     6421 tccttgaagt cctcgtaggg gtaatcctcg gggatgttga cggtcttgct gatcgaggtg
     6481 tccacatagg gctgcaccgc ctgcatcatc gccacatgct cgtgcgcggt catttcgagc
     6541 gcgttgacga agtactcggg cagcgtctcg ggctgctcgg gcgttgcgcc gggatcgatc
     6601 aggctgcggt acaggcggaa cgcgtggtct tccaccatgt agctgctggt gctgccgtcg
     6661 tgctcgcgct tgttgcgctt gtagccccag ctgaaggccg gttcgatgcc gttgctggcg
     6721 ttgtcggcaa aggccaggct gacggtgccg gtgggcgcga tcgacagcag gtggctgttg
     6781 cggatgccat gctggcggat ggcggcctgg atgtcctcgg gcaggcggct ggcgaaggtg
     6841 ccctgggcca ggtagccgtc ggcctggaac ttgggaaacg gccccttttc cttggccagc
     6901 tcgaccgagg cgcggtaggc cgcgtcgcgc atgcgccggc cgatctcggc cgccatctgg
     6961 cgccccggct cgcggtcata gcgcagcttg agcatgctga gcgcattgcc caggccggtg
     7021 aagccgaccc cgatgcggcg cttggaggcg ctttcggcgc gctgctgatc gagcggccag
     7081 aaagtgacgt cgagcacgtt gtccagggcg cgcacctgca ccgccaccac ctgctcgaag
     7141 gcgtcgaagt cgaagctcgg ctcgccgccg gcgccgaacg ggtgacgcac gaagttggtc
     7201 aggatgatgg ggccgaggtc gcagcagccg tagggcggca gcggctgttc gccgcagttg
     7261 tgcacgtaca ggccgttggc gtcgaaggcg tgcacgtcgg cgacggtgac gtcatagaca
     7321 tcggcactgc catccggcgt gacggcgctg acggtggcgg tgaaacgttc gcggttcagt
     7381 tggcgctggt attgggccag ggcgccggtc aagcgcgccg ccttgtccgt gtcggcaaaa
     7441 ccgatgcgat cggcgaacag ggccaggttg tcgccgctga ccaccagttc gtgctgcgcc
     7501 gccgtgcggt agcaatccag gccaccgcgg ccatcgggca gcgcgcgcac gccggtcggg
     7561 cgccgctcgg ggtaaatcgt gctggcgatg cccaggcgca gcagcatgcg ctgcgccgcc
     7621 tgcaaggtgc ccagatcgct ttgcgccagg cgcacgctca cgcccttgtc ttgcgcgccc
     7681 tgcaccgagc cgtcggtgtc gaacagaccg cgcagcgcac cgatgtggaa cgccgacgag
     7741 gttttctcca tcgacggcgt gacgctcttg cgccccggcg tgaggccgtg gctcagtgcc
     7801 agatgccgca gcgcgccgct ggccatgcgg cgttctgcgc gaccgcggac cgcacgctgg
     7861 aagccgccga agtcggcgcg gtgcggcagg gtgcgggcgg ccgcctcggc ggcggcgatg
     7921 atgccgtcgg cgccgctcag cgcatgcgcc ggcgcgcctt gcgccacctg gcgcagctcg
     7981 ggcgcccaga ccgagatgac ggccttgtcc tccttcagca caccatcgcc gaggagcagg
     8041 ccaagcaggt agccctcggc ctcggtgccc gcgccgtccc agccttgcag cgtgcggtgc
     8101 tgctgcagca tgatttcatc gcccgggcgc agctggccgg cgggctccca cgcactctcg
     8161 atcacgtagc gtgtcttgcg cgtgacgcgg cgcacgcggt ggtccgcggt caggcgcagg
     8221 ctgtgccctt cgcgggtttg caggtggacc accggtttgt ggccggtggc gaagaagccc
     8281 tggctgagca gcggatgggc cgcgccatcg acgatggcgt tgaagggccg acccatcagg
     8341 tcggccacct gggcggggcc atcctcggtc atgacccagg tatcggcggt gacgcagggg
     8401 ttggtggcct cgatctgctc ggtgtagttg aggttgttgt cgcgcccgat ctggtcgaga
     8461 aacaggatgc ccggttcggc aaaatcgtag gccgacttca tgatggtgtc ccacagctcg
     8521 cgcgccggca ccttgcggta cacccacaga ccgtcggcgc gctggtaggc ccccttggcc
     8581 ttgaccttgt cgcccggctc ggccttgtgc gccagctccc agtcgctgcc gtcgatcacg
     8641 gcctgcatga aggcgtcgga cacgcccacg gagacgttga agttgttcca gcgcccgggc
     8701 gtgcgcttgg cggtgatgaa ctccagcaca tcggggtgat cgatgcgcag cacgcccatc
     8761 tgcgcgccgc ggcgcgagcc ggcgctttcg accgtcgagc acgactgatc gaacacgttc
     8821 atgtagctgc acgggccgct ggcgaacgag tgcgtgccct tgacgaaggc gcccttgggg
     8881 cggatgcggc tgaaatcgta gcccacgccg ccgccgcggc gcatggtttc ggcggcctcg
     8941 cgcagagcct cgtagatgcc ggggtagccg tcggcgtcgc ggccctggat ggcgtcgccc
     9001 accggctgca cgaagcagtt gatcagcgtg gcctggatgt cggtgccggc ggcgctcatg
     9061 atgcgaccgg caccgatggc gccggcatgc aggttggcca gaaacttctg ttcccattcg
     9121 gcgcgcagct cgggcttttc gacgctggcc agggcgcgcg ccacccggcc gtagagctgg
     9181 tcgagcccgg tttcaccggg cttgaaatac ttctcggcca gcacgtcgcg gctgatcggc
     9241 tgcgcgctca gggcatgctg cgcgccgggc agggaatctc ttttcatgca ctcatctcct
     9301 cggatcgaca ggtcgacctg ggcacgcccg gtcgtggctg ccgacattct tgcacctccg
     9361 gaattcgcca tccgggacgc aactgtgcta cctgcgccac aggccctccg gatagctatg
     9421 aaatcaggag ctgcttgcgc tgatcagaca agcgccaggg ccagtttttt cgcaacttgc
     9481 tgaagccgga accgagcgcc ccgaagtgcg ctgcaccagt gccgcccggc tggccagcca
     9541 gggccacggc gcgcacggaa acgccgcgcc gccgcagccc ggcggctcag cccgtgcgcc
     9601 cgtagccgtc gcgcagatcg cgcttgagca ccttgccgat cgggctgcgc ggcagttcgg
     9661 gcagcaggtg caggtcggcc aggcgctggg tcttgccggc gcgctggttg aaccagtgca
     9721 tcagggcggc cgcttcgacc gcctgcccgg gctgcggcac gacaaacgcc accggcgttt
     9781 cgccccaccg ctcggagggc acaccgacca ccgccacgtc ggccaccgcc gggtgctggc
     9841 gcaactgggc ctccagatcg ctcgggtaga tgttgaaccc gccgctgatg atcatgtcct
     9901 tgcgccggtc cagcagcgtc agaaagcctt cggcgtcgaa gcgccccaca tcgccggtgc
     9961 ggatgaagcg cttgccggcg gcgtcgaacc actcggcctc gcgggttttg tcgggctggt
    10021 tgtggtagcc ggtcatcatg ccgggcgagt ggccgaccac ttcgccggcc tcgcccgagg
    10081 ccacctcgcg cccttgctcg tcgatcagcc gaatgtcgtg accttgggcc ggccggccca
    10141 cggtgtgcag tttgtccgga tgcaggtggg cgtccaggat gcaggtgccg ccgccttcgg
    10201 tcatgccgta gaactcggtc aggccgcccg gccagcgcgc cagcacgtcg gccttgaggg
    10261 ccgccgaaaa cggtgcgctg gtgcagaact tgacccggaa cgcggacaga tcgaatgcgc
    10321 cgaactgcgg cagcgccatg atgcgctggt actgcaccgg caccagcatg gtgtgcgtga
    10381 cgcggtgctg ctgcgccagt tgcaggtaac gcagggcgtc gaacttgggc atcagcacca
    10441 ccgcgccgcc atagcccagg gtggggaaga acaccaccag cgtggtgttg gagtacagcg
    10501 gcgtggacag cagcgtgacg ccgtgcggcc cgtagtcgta caccgcgccg cggcgcacgt
    10561 gcatccagcg catgccgtgc gactgcacga tgcccttggg cgtgcccgtg gtgccggacg
    10621 aatagatgat gttgaagggc gcttcgggca ccagcgccac cggggcgggt tgagccccca
    10681 ccggcgccag ccagtcggca aaggcgcgcc cgggcgccac gccatcgagc gaaacccgcg
    10741 gcagccggtc cggcccgggc aggtgctggg ccggcgcggt ggcgtcgaga aacagcacgc
    10801 gcgcccgcgc atcggccacc atggcggcca gcgcctgcgg cgtcgatgac ggcgccagcg
    10861 gcgccaccac caccccggcg cgcagcgcgc ccaggtacag cgccgcatac ggcacggcgt
    10921 tgagcccgca caccacgatc gcctcgcccg gcgacacacc atcgcgctgc agcgccgcgg
    10981 ccacgcggtc catcagcgcg tccagctcac catagctcag ctgctgccga tcgtcgatca
    11041 gggccgggtg ggccgggtcg cgccgcgcat gctcgcggat caggtcggca acggcacgga
    11101 aagcgggttc ggcggacatg gcggacgggg ctgcggtcgg tcggcaccag ggcgtgttca
    11161 gtcgttcagg ccgagcgact gcacgatgga tttctcggcc tcgaaattcc tgcgtgcgaa
    11221 ctcggcatag gcctggtggt cgcggtagtc ggtgcgcaca ccataggtct tgatgacgcg
    11281 ctgcatgtcc ggatcgtcca gcgcggcctt gaccgcggcg tcaagcttct ggacgatggc
    11341 cgacggcagg cccttcgggc cggccaaccc cagcggcgac ggcaccacca cgtcgtagcc
    11401 ggcttccctg aacgtgggca cgcctgcgaa ggccggcggg cgctggtcgg ccgcgatggc
    11461 cagcgcccgt accttgccgg actccatgaa gctcagaacg ccgttggtgc tgacgatggt
    11521 cttcacatga ccgcccagca gggccgcggt ggactcggaa tcgcccttga acggcacatg
    11581 cacgaacgac acgccggcgg ccttggccaa caacgccatc gccacctggt tgccggtgaa
    11641 cttgccgggc gtgccgtagt ccacagcgtc cttccggccc cgggcgtgct cgaccacgtc
    11701 cttgaccgac ttgaacggtg agtcggccgc cacggcaaga atgaagtcgt aggcggtgaa
    11761 cgaggcgatg taggtcagat cgcggatcgg gtcgtagggg gtcttctgaa cgtagggctg
    11821 gcggaacacg ctgaccggca ccagcgtcag gcggtagccg tcgggcttgg cgcgcatcag
    11881 gtcgaccacg cccagcgtgc cacccgcacc ggacttgttc tccaccacca ccggctgctt
    11941 gagttgcctg gacaggctgg cagccagcgc gcgacccagc acgtcggtgg tgccacccgg
    12001 cgcgtagggc acgatcatgg tgatcgactg gttgggatag tcggcctgag cctgaaccgc
    12061 ggtggcgtac cacgccccca tgcatgccac caccagggtg agcagacgtc gcttcatggt
    12121 tgctccgctc caaaacccga gatgttgcct ttgtcgcgat cccgcggcaa ccctcggtga
    12181 cgatccgacc ggtgccggtg gcgtcgagcg cccacccgca ccaagccggc ggcaccgtgg
    12241 cccacggccg cctcagaaga tctcgaacag tccggccgcg cccatgcccc cgcctatgca
    12301 catggtcacc acgccccatt tcacgcgctc gccgcgtgcc ttgcggcgct ggccctcgag
    12361 caggatgtgg ccggccaggc gcgcgcccgt catgccaaac gggtggccaa tcgagatggc
    12421 gccgccgttg acgttcaggc gctccatggg gatggcaagc cgacgctggc agtacagcgc
    12481 ctgcgaggca aaggcctcgt tcaattccca caggtcgatg tcctgcacgg tgaggcccgc
    12541 gcgctcgagc aggcgcggca cggcaaacac cgggcctatg cccatctcgt cgggctcgca
    12601 gccggcgatg gccaggccgc ggaaggcgcc caggggctgc aggccacgcc tttcggcctc
    12661 ttgggcctcc atcagcacgc aggcggccgc gccgtccgag agctgcgagg cattgcccgc
    12721 ggtgacgaac ttgcccgcgc ccttgacggg ctcgagcccc gccaggcctg ccagcgtggt
    12781 cgtggggcgg ttgcagttgt ccatggtcac ggtcacttcc ttgtgcgtga cctcgcccgt
    12841 gtccttgttc ttctcggcca tggtggtctt gcagggcacg atttcgttgt cgaatacacc
    12901 gccggcctgg gcggcggccg tgcgctgctg gctttgcagc gcaaaggcgt cctggtcctc
    12961 gcgcgagatg ccgtagcgcg ccgccaccac gtcggcggtg tcgatcatct ccatgaacag
    13021 ctggggcttg tgctcgacca gccaggggtc caggtgggcc gtgccgccgc ggcccgcggg
    13081 gttgagcagc gagatgctct ccgcgccgcc cgcgaccatg gccggcacgc cgtccatcac
    13141 gatgcgcccg gccgccacgg cgatggcctg caggcccgag gagcagaagc ggttgatcac
    13201 cgtgccgccc accgtcaccg gcaggccggc gcgcagcgcg gtctggcggc cgatgttgcg
    13261 gcccatcgtg ccctcggggt agccgcagcc ccagatcagg tcctcgatca ggccgggctc
    13321 tatgcccgcg cgctcgacgg cggcgcgcac cgcatgggcg cccagttgcg ggccgggcgt
    13381 gatgttgaac tcgccgcggt gcgacttggc cagcggcgtg cgggcggtgg aaacaatgac
    13441 ggcttggcgc atgggaggct ccttgggaat ctggtgttac tattgaattg atagctggtt
    13501 gcgcttgatg ggcgggcgct agcggcattt ttcttcacaa accaacagcg ctgcgtagct
    13561 accccctccc cctctggggg agggttgggg tgggggccag cggcgctcag aacctcggtg
    13621 ccggcaagat gccggcgctc ccggccccca tcccagcctt ccctcagagg gggaaggagc
    13681 gggtcatcag ccgcgctggt tcaggctggc aaagtttttg ccctcggcca cgagtctttt
    13741 cagcagtgcc gcgggctgcc agaactgcgc gtcctccctg gcaaattcct cgatgtcggc
    13801 caggaccttg ggcaggccct gcatgtcggc ccacagcatg gggccgccgc ggtggcgcgg
    13861 gaagccgtag ccgaacagaa aggtcacgtc gatgtcgagc gggcgcaggg caatgccctc
    13921 ctcgagcacc ttggcgccct cgttgaccat ggccgccatg tagcggcgca tgatctcctc
    13981 gtgcgtgaag gcgcgcggcg cgatgccttt cttttgccgc tcggcgtcca cgatggccag
    14041 cacctcgggg tcttgctggc ccacgcgcgc gccctcgggg tagaggtacc agccacgccc
    14101 ggtcttctgg ccgtaccagc cgcgctcggc gatgcggtcg gcaatctcca catagcgcgc
    14161 cctggggtcg cgcgtggcgg ccttgcgttt gcgcgtggcc cagcctatgt cgccgccggc
    14221 cagatccgac acctggaacg gtcccatggg gtagccaaag gcgcgcaccg cggcgtcgat
    14281 ctcgtagggg ctggcgccat cctcgagcac atggttggcc gcctcgcggt acacggccag
    14341 gatgcggttg ccgatgaagc cgtcgcacac gccggcgcgc accggcacct tcttcatgcg
    14401 cttggccagc tcgaatgccg tgaccaccac gtcgggggct acccttgcgg gcacgacgat
    14461 ctccagcagc cgcatgatgt tggccgggct gaagaaatgc aggccgatca cgtcctgcgg
    14521 gcgggacgtc actgcggcaa tggcgtcgat gtcgaggtag gaggtgttgg tcgcgagcac
    14581 gcagccgctc ttgcacacgc ggtcgagctc ccggaacacg gcctgcttga ccgctatgtc
    14641 ctcgaacacg gcctcgatca ccaggtccac gtcggcaatg tcctcatagc gcgtcgaggg
    14701 ctggtagcgc gccatggtgg cggccttggc ctcggccgtc atgcggccct tggcgatcag
    14761 gccgtcgtag accttctcca cgttcttctg gccgcgcgca atcgactcgg cgtcgcgctc
    14821 gatcatcacc accgacaggc cggcgtcgag cgcagacacc gtgatgcccg cgcccatggt
    14881 gccgccgccg atgatggcca gcttcgcaaa cgggcgcggc gcagccgcct tggcctcagg
    14941 gatcttggcg gtctcgcgct cggcgaagaa cgcatggacc aggccggcgc gctgcgggct
    15001 gttcaggcat tcgacgaaga gctcgcgctc gcgcttcgca ccctcggcaa acggcgtctg
    15061 cacggccaca cgcacgcagt cgatgattcg ggcgggcgag aacaggccgc gcgtcttctt
    15121 ggccgtgtcc tgcgccagct ggtcgagcgc ggccagggtc tgggccttgt cgccgggcac
    15181 ggccagcgcg ctggtgcgcc gcggcgcggt accggccgcg agcagctcgc gcacataggc
    15241 caggcccgcg gccagcgggt cgtcgccctc caccagccgg tccaccaggc ctgcggccag
    15301 cgcggccttg gccgagaggt gtttgcccga cagcatgagc tcggccgcgg gcagcgcgcc
    15361 catcaggcgc gggctgcgct gcgtgccgcc ggcgccgggc agcaggccca gggcgacctc
    15421 aggcaggccc agcctggccg ctggcagggc caggcggtaa tgcgtggcca tggccacctc
    15481 cagcccgccg cccagcgccg ggccgtggat ggcgcagacg acgagcttgt cgcaggcctc
    15541 catgcggttg atggtttcgg gcagcgaggg cggctgcggc ggcttgccga actcgcggat
    15601 gtcggcaccg gcgatgaagg ccttgcccgc gcccacgatg agcatggcgc gcgcctgggc
    15661 ggtccgctcg agctcctcga cggcggccag caggccggcg cgcacggcct ggcccagtgc
    15721 gttgacgggg gggttgtcga tggtgacgac cagcacgtca ccgtcgaggc gggtctgcac
    15781 cgggccggcg ccctgtgaag cgttcatgcg cttgtctcca tgcgagttgt aggcatccct
    15841 gcattctccc catgggtggt ttttttgaca atcacatcag cgattgacaa actgtcaaac
    15901 ggcatttgac attgcaagca tggaccttca gtcgctcacc ctgctggtcg agatcatcga
    15961 cagcggcaac ctcagccagg ccgcgcgcaa gctgcgcatg acacgcgcca acgtcagcta
    16021 ccacctgacg caactggagc aatccgtcgg cgcgcagctg ctgcggcgca gcacgcgcca
    16081 cgtcgagccg accgagatcg gcctgcgcct gtatgaacat ggccgcgcca ttcagaacga
    16141 aatgctggcc gcacgcgaga ccgtgacccg tctgggccag ggcctgatgg ggcgcgtggg
    16201 catcagcatg cccagcggtt acgggcagat ggtgatgtcg cactggctga tcgagttcaa
    16261 gcggcgctac cccggcatcg tgctcgacgt ggtgttcgag aaccgggtcg acgtgctgcg
    16321 cgacgacgtg gacatcgcgg tgcgcgtgct gcccgagccg ccgccacatc tggtggcacg
    16381 cgagctggac gaggtgcgct acctggcctg cgcctcgtcg gcctttgccc gcacccatgg
    16441 cctgcccacc cgcctggagc agctgaccac gctgccgctg atcaccgcca gcgtggtcgg
    16501 gcggcagctg tcgctgcgcg gctaccggca ggacgagcgg cacgaggtgc tgctggcgcc
    16561 gaccctgatc tcggaccact acccgtttct gcgccagggc attctggccg ggctgggtct
    16621 gggcctggtg cccgactatg tggtgcagga cgtggttgcc agcggtgcgg tggtgaccgc
    16681 gctggacgac ttccggctca gcatcttcgg cacccgcatg tacctgctgc acctgcccgg
    16741 caggcaccag acgcgggcgg tgcgcacctg catcgacttt ctgctggcgc gcgccgccgg
    16801 ccagtccgcg cccccgacgc ccggcgagcc accggcctga cccgcggcgt tgccggcgca
    16861 accgttccgc cggtcaccca attactatca tttcaatagc tgctggcgct tgcctgtcaa
    16921 gcgccggagc ctgattttaa ttaaaggtgc gctgagcccg tccggcgcgc gtcgcatcgc
    16981 agggacgggt tcagcatcgc ctcagggctg tgcctggaac gcacgcagcg ccgcgatgcg
    17041 ctcttgcatc ggcgggtggg tggaaaacag cttgccgatg ccgccggcga tgcccatggc
    17101 cgccaggttc ttgggcattt cggcgccttc ggcatgcatg ccgcccaggc gcgccagggc
    17161 gttgatcatc gagttgggct ggcccatcat ctgcgccgcg ccgcggtcgg cgcggaactc
    17221 gcgctggcgc gagaaccagg ccacgatgat gccggccagc acgccgaaca ggatgtcgag
    17281 cacgatggtc gtcacgtagt agccgatgcc cgggccgctg ctttcgctgt cgcggcgcag
    17341 gaagccatcg accagcgaac cgatgacgcg gctggcgaac accacgaagg tgttcattac
    17401 cccctgaatg agggccatgg tgaccatgtc gccgttggcc acgtgggcca cctcgtggcc
    17461 gatgacggct tcgacctcct cgcgcgtcat gccgcgcagc aggcccgtgg acaccgccac
    17521 cagggccgaa ttcttgaagg cgccggtggc gaaggcgttg ggttcgccct cgtagatgcc
    17581 gacctcgggc atgccgatgc cggcctgatc ggcgaacttg cgcacggtgt cgacgatcca
    17641 ggcctcgtcg gcgttgcgcg gctgcgcgat gacctgcacg cccatgctca tcttggccat
    17701 cgacttgctc atcagcagcg acacgatggc gccggaaaag cccaccacca gcgaaaacac
    17761 catcaactgc ccggtgttgg tgccggtgta gcggcccacg cccagcaggc tggtgacgat
    17821 gcccaccacc accatcacgg ccaggttggt cagaacaaac agcaggatgc gtttcatgaa
    17881 ttcccttggt tgacaacagg tgacctgaaa tgcccgcccc gtgcgccgcg cgcaagcggc
    17941 aagcgcttcg gcgtcgccgc aaggtcggca caggcgtgca tgttaggggc gattggagca
    18001 gattcaaggc gccgggttcc cacgggcgcc gggttggcca ggcgctggcg cttggcctac
    18061 actgaagcac ccccgaggag cacacgtgaa caccctggac tggctcggcc gcctggtggc
    18121 catcgacacc accagccgca actccaacct cggcctgatc gagaccgtgc gcgacgcgct
    18181 cacggcccag ggcctgaagc cctggctggc cgccaacgcc gacggcagca aggccaacct
    18241 gttctgcacc ctgcccgcgg cggacggcca gacgcagggc ggcatcattc tgtcgggcca
    18301 caccgacgtg gtgccggtgg acgggcagga ctgggccagc gacccgttcc agctcaccga
    18361 ccgcggcgac ggccgcctgt acgcgcgcgg cagctgcgac atgaaaggtt tcatcgccag
    18421 cagcctggcg ctggtgcccg aggcgctggc ggcgccgcgc cgcaaaccgc tgcacctggc
    18481 actgtcgtac gacgaagaag tcggctgcgt cggcgcgccg gtgatgctgg ccgagttgaa
    18541 ccggcgcggc atccgcgccg acggctgcgt ggtgggcgag ccgacctcga tgaagccggt
    18601 ggtggcacac aagggcggct acttcttccg ctgcaccgtg catggcaagg cggcgcactc
    18661 ctcgctcacg ccgcagggct gcaacgccat cgagcacgcg gcgcggctga tctgccacat
    18721 ccgcgacctg gccgacgcct ggcgcgcgca gggcccgttc gacgccgcct tcgacgtgcc
    18781 gcacagcacc atcaccacca acctgatcgc cggcgggatc gccatcaaca ccgtgcccga
    18841 cgcctgcagc ttcagctacg acttccgcca cctgcccggc cacgacgcca gcggcatcca
    18901 ggcccaggtg cagcgctacg tggacgacga gttgctgccg cgcatgcggc gcgagtacgc
    18961 cggcgcgcgg gtggacatcg aatgcggcgc cgccgtgccc accctggacg ccagcgagca
    19021 ggccgccatc acccagctgg tgcgcgccct cacgcaggac cagggggtgc gcaaggtggc
    19081 ctatggcacc gaggccggcc agtttgccca ggccggcatc ccggccatcc tgtgcgggcc
    19141 gggatcgatc gagcaggcgc acaagcccga tgaatacgtc gagcacgcgc agctcgcgct
    19201 gtgcgatcgc tttctgcgcc aggtgctggc cacgctgtga cgcgccgcca cgcctcgtga
    19261 gccccaccac gcccgccgcc tgcgcccgcc tgatcgccca gctgcagcac gacctgcggg
    19321 ccagcggcgc ggccgacgtg cagtgcctcg aaacccacat ttcctggttg ctgctggcgg
    19381 gcgagcatgc ctacaagatc aagaagccgc tgacgctgga cttcctggac ttcggcaccc
    19441 tggcgcagcg ccgcgccgcc tgcgaggagg agctgcgcat caaccgccgc agcgcgcccg
    19501 agctgtacct ggaggtgctg ccgatcaccg gcacgccggc ggcgccgcgc ctgggtggcg
    19561 atgccgcgca ggcgatcgaa tgggccgtgc acatgcgccg ctttccgccc ggcgccctgc
    19621 tggccgagcg cgccgcgcgc ggcgccctgg ggcccgtcca gatcgacgcc ctggcgcgtg
    19681 cggtggccac cttccaggcc caggccgcac aggcaccggc cgacagcccc tggggccatg
    19741 cggccgatct ggcgcggctg gcgcacgaca actttgcccc cctggcggcg ctgggcgcgg
    19801 gcaccgcgct ggccggcacg ctgcagcggc tggcgcactg gagcgagacc gagggccaac
    19861 gcctggcgcc gctgatggcc gcgcgccgcg ccgccggccg ggtgcgcgaa ggccacggcg
    19921 acctgcatct gggcaacctg ctgtggctgg agggccgtgc gcgcctgttc gacgccatcg
    19981 aattcgatcc agccctgcgc tggatcgacg tcgcctgcga catcgccttc gtgttcatgg
    20041 acctgcacgc ccatggcctg gccgcgctgg cctggcgctt tctgaatgcg tggctggagc
    20101 acagcggcga cttcggcgcc gtggcgcggc tgccctacga cgccgtctac cgcgccctgg
    20161 tgcgcgccaa ggtggcggca ctgcgcgccg ggcagggcca gggtggcgtc gcgcgcaccg
    20221 ccgcagaagc cgaagcgcgc cgctacgtgc agctggcggc gcagctggcc gcgccgcgcc
    20281 ggccctggct ggcgctcacc gtgggcgtca gcggctcggg caagagcagc cagacgcaga
    20341 ccctgatcga gcggcgcgcc ctggtgcgcc tgcgcgccga cgtcgagcgc aagcgcctgt
    20401 tcggcctggc gcccgaagcc gccagcgccg gcgtgcccgg cggcatctac ggcgaagcca
    20461 ccagcgaccg tctgttcagc cacctgcggg accaggcgcg cgcgctgctg caggccggcc
    20521 agccggtgct ggtggacgcc accttcatcc gccgcgcgcg gcgcgcgccc ttcatcgcgc
    20581 tggccgaaga gctgggcctg ccctggcgcc tgctggcctt cgacgccccg gagcaggtgc
    20641 tgcgccagcg cgtgcgtgag cgcgccgcgc agggcggcga cgcctccgag gccgacgaag
    20701 cggtgctgca ccgccagctg gcccagcgcc agccgctgac ggcgaacgaa gccagccacg
    20761 cgctgaccat cgacgcccgc cagccggtcg actgggcggc ctgggacagc gccctgcccg
    20821 gcgcctgaaa tactccaaat tcaatagctg cttgcgctgt ctggaagcgc cctgaagcac
    20881 gatttcatct cgaattcagg cgcaccccgg ctcctacggt gtcgcgtcct cacaccgggc
    20941 ccatgacacc ggctgcgccg gcatgaccca tgccccatgg gtcagcgaaa ggcagcgagg
    21001 tcatctgtcg ttcgtcattg tgaaaaagcg ttcgcttcca cccgcagccc gggcgcggcc
    21061 gcgcggccgg aacacctcgg cgtcgtcata gaaggccggc tgcgcgttgt ccggccacca
    21121 gcccggcacg cccagcagcg gcagcggcac gaagggcttg cgcgccagct tgtcgggcgt
    21181 caggtcggcc gccagccagg cgtccagcgc cgccagctcg cccgcggcgt cgaacggcag
    21241 cgccacgcgc cacacatggg cggtgatcga cttgtagggc gaaagcagct tttccagcgc
    21301 ggcgtggccg aacaccagca ggctggcctg gccccacagc ggccgcagcg gcccgaacag
    21361 ctcggcccag cgccgctcga ccagcgcctg ccacagcgcg tcgggcgcgt gcagcagggc
    21421 ggcgttttcg tcgaacacgg tgcaggcatc gcgcaccggt ccgcggcgcg cgccgacgcc
    21481 ggcgcgagcg atctcgtcgg cctgcagcgc gttcagccgg cccttggtgc gcggcaggcg
    21541 cagccacacc agaccgttga acagatcgtg ggcgttgtcg cgcgtgggca cctggcgccg
    21601 ggcgcggatg aaggcttcat acgcctggcc ctccggcagc gcctgctggc ccacaaagcg
    21661 caccggc
//
LOCUS       DAOQTZ010000042        21635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2846_length_21635_cov_13.525209, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000042 DAOQTZ010000000
VERSION     DAOQTZ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 21635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21635
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2846_length_21635_cov_13.525209"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="PKN51_06800"
     CDS             <1..1383
                     /locus_tag="PKN51_06800"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNT84845.1"
                     /translation="GDFECVKAIAEVIKDSTVCSLSRANDRDIARAAEALQGAARGRI
                     HTFIATSPLHMEKKLRMTPDEVFEQARLAVRFARNLRDDIEFSAEDGYRSDIDFLARV
                     CETVIKEGATTINIPDTVGYAIPELYGHFIKTLREKVPNSDQAIWSVHCHNDLGMAVA
                     NSLAGVKLGGARQVECTINGLGERAGNCSLEEVVMAVKTRRDHFGLDVGIDTSQIVPA
                     SRMVSHTTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWSANKIV
                     LGKLSGRTAFKQRLQELGVVLESEGEVMAAFAKFKDLADRKSEIFDEDILALVSDESV
                     TAEKEQYGFISLAQRSETGETPHAEIVFTIDGREVRGVSDGNGPVDASLKAIESQVSS
                     GAEMVLYSVNAISGNTESQGEVTVRLQNSGRIVNGVGADPDIVVASAKAYLSALNKLH
                     SKADRVAAQG"
     gene            1539..2705
                     /locus_tag="PKN51_06805"
     CDS             1539..2705
                     /locus_tag="PKN51_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNT84846.1"
                     /translation="MTKHFFVLGIAALLSLSALSTASAAPAKKSVKAAVAHKSASAPA
                     ARSGRPALARQGRETRRTAAQPSGRTTARDTRGARLTLARQESRRTSGLSARQTRLSL
                     RGASTSQVVQARAPRLSFGQMAGLHAVDDPLDLKSSVALVLDQDTQEVLLSKNDHAVL
                     PIASLTKLMTGLVVADARLPLDELVTITEEDIDTEKGSRSRLAVGTQLTRGELMHLAL
                     MSSENRAAHALGRTYPGGLQRFVGLMNARALMLGMKDTRYVEPTGLSSGNRSTAHDLA
                     TLVAAAAREPLLRQLSTSTGYEVAVGSRLVQYNNTNRLVHDPEWQIGLQKTGYISEAG
                     QCLVMQARVAGRKLIMVFLDSSGKHSRLADAERVRRWVEVQRPANTVAGSPNAV"
     gene            complement(2970..3752)
                     /locus_tag="PKN51_06810"
     CDS             complement(2970..3752)
                     /locus_tag="PKN51_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNT84847.1"
                     /translation="MRRPRPDAHQTPTIQVIERMFALIDVLASREEPMSLKDISEQTG
                     LHPSTTHRILNDLTIGRFVDRPEAGSYRLGMRLLELGNLVKARLNVRDAALAPMRELH
                     RLIQQPVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLASDDPQ
                     RVRAYATRTGLAGHTRNSITQLPALERELIGARHAGVARDNEELELGVRCMAAGIYDD
                     QGKLVAGLSISAPADRLDDAWLPKLKQTAQQISAALGFDERR"
     gene            complement(3758..4504)
                     /locus_tag="PKN51_06815"
     CDS             complement(3758..4504)
                     /locus_tag="PKN51_06815"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNT84848.1"
                     /translation="MTHLKDLPLQALQFYATAPYPCSYLPGRQARSQVATPNHLINND
                     TYSGLVQRGFRRSGLFTYRPYCDGCNACVPVRVRAGEFRPDRSQRRARARHRDLQVRV
                     LKLGYVPEHYRLYLRYQAGRHTGGGMDHDSVDQYTQFLLQSNVNSRLVEFRDPDRDGA
                     PGALRMVAIVDVLGDGLSAVYTFYDPDPRASYGTYAVLWQIEQAIRLGLPHVYLGYWI
                     EGSRKMMYKARFQPLERLEHGQWQRAPRPA"
     gene            complement(4501..5268)
                     /gene="aat"
                     /locus_tag="PKN51_06820"
     CDS             complement(4501..5268)
                     /gene="aat"
                     /locus_tag="PKN51_06820"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNT84849.1"
                     /translation="MNAWPPLTQLKPGDPFPPLSQAWPAHSPAPGLVAVGGALDVPSL
                     LAAYAATIFPWFSEGEPILWWSPDPRMVLAVDRFRLHRSLRRVVQRFRASAGCEIRIN
                     SAFDAVIAACAGATRQGQSGTWIGPQIRHAYGALHRAGHAFSVETWIDGELRAGLYGV
                     TIGRAVFGESMFTQVTDGSKIALAALIALCRAQGLPLIDCQQNTRHLAFMGAAEMPRA
                     AFADAVRRLVHEPAPAWRFEPVYWDALLPPAPLTADR"
     gene            complement(5268..5813)
                     /locus_tag="PKN51_06825"
     CDS             complement(5268..5813)
                     /locus_tag="PKN51_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNT84850.1"
                     /translation="MLRPPIKHCRQCGTPVQYRIPDDGDTRERAVCPACGTIHYVNPL
                     LVVGTVPYLGERVLLCQRAIEPRWGKWTLPAGFMELDESMAQGAARETAEEAGAQIEM
                     GPFFSAMSIPRIGQVHVFYLARLLSEHFDPGPESAQVHLFTEDEVPWDELAFTAVRRT
                     LECFFADRRRGQFGVHDFDIA"
     gene            6042..6488
                     /locus_tag="PKN51_06830"
     CDS             6042..6488
                     /locus_tag="PKN51_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT84851.1"
                     /translation="MSDRFAFERAPGHLIRRAHQIAVAVFTEEVGAHGVTPVQFAILN
                     ALMQQPGVDQVTLAQRVAFDAATIGSVIGRLEKKGWLRRQADAADRRRRLLWLTAEGE
                     RAALAMIPSVGQVQQRLLQGLDHAERQQLLALLDKLVRHHPRSLSG"
     gene            6542..7255
                     /locus_tag="PKN51_06835"
     CDS             6542..7255
                     /locus_tag="PKN51_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT84852.1"
                     /translation="MTRRIVVPCRGAVVAVTAGALWLAGCAGMTETQRSTAIGAGVGA
                     VAGGVIGHGRGAVIGAGVGALGGYAWSRYMEGKRQQMQQATAGTGVEVTQTPDNQLKL
                     HIPNDLSFDTNRADLQPRLRPILDQFAQGLGQQPGLEVFIVGHADASGTDAINDPLSL
                     ARANSVRDYLLSRGVDARSMATEGRGSREPLASNDSPAGRARNRRVEIFLAERASVAA
                     APPAAPPPAYSTPVGQPVR"
     gene            7291..7929
                     /gene="maiA"
                     /locus_tag="PKN51_06840"
     CDS             7291..7929
                     /gene="maiA"
                     /locus_tag="PKN51_06840"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HNT84853.1"
                     /translation="MKLYNYFRSSASFRVRIALHLKGLAYDYLPIHLAKGEQRQPAFA
                     AVTAEGLVPVLQTDDEHRLTQSMAIIEYLDETHPQPPLLPADALGRARVRALAQIVAC
                     EIHPLNNLRVLKYLVHDLKASEDAKNAWYRHWVRLGLQAYEGQLATQTGRYSHGDTPT
                     LADCCLVPQIFNARRFDCDLSGLPRTMAVFDACMQLQAFQNAQPSACPDAEA"
     gene            7929..8756
                     /gene="pgeF"
                     /locus_tag="PKN51_06845"
     CDS             7929..8756
                     /gene="pgeF"
                     /locus_tag="PKN51_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNT84854.1"
                     /translation="MPAGLPAAAGPWLIPDWPLPPGVRALFTLRGQSAADGASDPPWA
                     HLNLGEHVGDATAAVAANRARLRAALGARPVFMHQVHGTHVLTLTPDCGDGTSADAAV
                     TDQPGLACTVMVADCLPVLLTDTRGRAVAAAHAGWRGLAAGVLEQTFKRLKALALDQQ
                     APPAIKMEADEWLAWLGPCIGPTAFEVGAEVHAAFLAGDAGAGSCFRPRPDGKFLADL
                     AALARRRLAALGVTRVYGNDGGPAWCTVGQPARYHSYRRDERALGGSGRMAACIWRV"
     gene            8947..10662
                     /gene="phaC"
                     /locus_tag="PKN51_06850"
     CDS             8947..10662
                     /gene="phaC"
                     /locus_tag="PKN51_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNT84855.1"
                     /translation="MSAGFPESMVGAAFGQGFAETWMKAFQHFQNMDLGKGAGEAPSI
                     DVPQLKFSPEALAKLQQDYVAEAAQLWNQTLQANPPLKDKRFAGEAWASNPLASFTAA
                     VYLLNARTLLAMADAVEGDAKARNRVRFAVEQWMAAAAPSNFLAFNPDAQRLAIDTHG
                     ASLAKGVANMLQDMKQGHVSMTDESVFQVGKNVATTEGAVVFENDLFQLIEYKPLTDK
                     VHEKPFLLVPPCINKFYILDLQPTNSFIRYAVSQGMRTFVVSWRNPDESLATATWDDY
                     IERAIIQAIRTVQDISGARQINALGFCVGGTMLTTGLAVLAARGEKPVSAATLLTTLI
                     DFTDTGILDVFIDEQMVRYREMQMGKGGLMKGQDMASTFSFLRPNDLVWNYVVGNYLK
                     GETPPPFDLLYWNSDSTNLPGPFYAWYLRNTYLENNLVKPGKATVCGEKIDLRKLDIP
                     FYLYGSREDHIVPIGGAYGSTQALPGKKRFVMGSSGHIAGVINPPAARKRSHWRRDDG
                     KFPKDVNDWIAGATEHPGSWWDDWADWLKSHAGKQIAAPKTYGRGTAYRAIEPAPGRY
                     VKVKA"
     gene            10755..11936
                     /locus_tag="PKN51_06855"
     CDS             10755..11936
                     /locus_tag="PKN51_06855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNT84856.1"
                     /translation="MEDIVIVSAARTAVGKFGGSLAKVPATELGSIAIRAALERAKVG
                     LDQVGEVILGQVLAAGCGQNPARQALMKAGIAKETPAMTINAVCGSGLKSVMLAAQAV
                     ATGDSEIVVAGGQENMSASPHVLLGSRDGQRMGDWKMIDSMIVDGLWDVYNQYHMGIT
                     AENVARQEGITRDMQDALALASQQKAAAAQAAGKFKDEIVPVSIPQRKGDPVVFATDE
                     YINTKTSAEALAGLRPAFDKAGSVTAGNASGINDGAAAVVVMSARKAAALGLKPLARI
                     ASYATSGLDPATMGLGPVPASQKALQRAGWKVGDVDLFELNEAFAAQACAVNKLLGAD
                     PTKVNVNGGAIAIGHPIGASGCRILVTLLHEMQRSGAKKGLAGLCIGGGMGVAMALER
                     A"
     gene            12070..12807
                     /gene="phbB"
                     /locus_tag="PKN51_06860"
     CDS             12070..12807
                     /gene="phbB"
                     /locus_tag="PKN51_06860"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNT84857.1"
                     /translation="MSQKVAYVTGGMGGIGTAICHRLHKAGFKVIAGCGPTRDYQKWL
                     DEQKALGFTFHASVGNVSDWQSTTDAFTKAKAEHGPIDVLVNNAGITRDRMFLKMTRE
                     DWDAVIDTNLNSMFNVTKQVVPDMVERGWGRIIQISSVNGEKGQAGQTNYSAAKAGMH
                     GFTMALAQELASKGVTVNTVSPGYIGTDMVRAIKPEVLEKIVATIPVKRLGTPEEIGS
                     IVAWLAGEDSGFTTGADFSCNGGLHMG"
     gene            12974..13990
                     /gene="dusA"
                     /locus_tag="PKN51_06865"
     CDS             12974..13990
                     /gene="dusA"
                     /locus_tag="PKN51_06865"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNT84858.1"
                     /translation="MPEAVSPWRLSVAPMLDWTDKHCRFLHRLLSRRTRLYTEMVTTG
                     ALLHGAERRHLRFDAVEHPLALQLGGSEAADLAACARIGARWGYDEINLNCGCPSERV
                     QRGAFGACLMAEPQLVADGVKAMVDAVDVPVSVKHRIGIDREESYDFVRDFVGTVAEA
                     GCRVFIVHARNAWLQGLSPKENREIPPLRHALVYRLKRDFPQLTIVLNGGVTSDAQIT
                     EHLQHVDGVMLGREAYHHPWMMSRWDAEFLGGAACALTQDEVEAEMVAYMARELAEDG
                     TPWSAIARHMLGLHHGRRGARLWRQVWSDHRLKARPAAEVAALARSHLAAGAHAGQGA
                     VAGA"
     gene            complement(14454..14864)
                     /locus_tag="PKN51_06870"
     CDS             complement(14454..14864)
                     /locus_tag="PKN51_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNT84859.1"
                     /translation="MSFAKGLATLAAGALFGFGLALSSMIRPEVVLSFLQGRDLGLAL
                     VMGGGVAVTLLAYKLLPRLLQRPLLGGSFGVHPSSWNRDTLRGAALFGVGWGLTGVCP
                     GPAIAGLGAGNLSLLWAVAGMALGALVQGWRADR"
     gene            complement(14861..15334)
                     /locus_tag="PKN51_06875"
     CDS             complement(14861..15334)
                     /locus_tag="PKN51_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNT84860.1"
                     /translation="MSILQTPFPVGWQHYLLGGLCLGAGVALLFLFTGRIGSMSTVFS
                     STWSYVVRRPFFQQARLVDTRGWRLLYALGLILGALAWWLWRGAGVAEGTQVPWWLLL
                     AGGFVAGYGARLGNGCTSGHGICGLGSLQWPSALAVLTFMATAFITANLVTRWIV"
     gene            complement(15425..15829)
                     /locus_tag="PKN51_06880"
     CDS             complement(15425..15829)
                     /locus_tag="PKN51_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNT84861.1"
                     /translation="MLANLTPFLLHWAITAFALWVAAHVFKGLRFDGGGALVVAALLL
                     GLANAVVRPLLVLLTLPLTVLSFGLFLLVINALVLMLVARLVRGFHLDGFWTAFWASI
                     FMSLLSIVLGLVLGGTPEFTVETMPAPGQRWL"
     gene            complement(15910..17733)
                     /locus_tag="PKN51_06885"
     CDS             complement(15910..17733)
                     /locus_tag="PKN51_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNT84862.1"
                     /translation="MSSPNDTAAAEAAKPSHFIRQAIEHDLQAGTYALRRWGGQPGDG
                     PSHQAGMADPARIRTRFPPEPNGYLHIGHAKSIWLNFSLAAEYGGVCHLRFDDTNPEK
                     EEQEYVDSIRDMVRWLGWDHHFADDPARPGVRQPHEYFASDYFDFMYRAAEHLIEAGH
                     AYVDEQSAEQIRASRGDFGTPGTDSPFRHRTPAENLARFREMRDGQHPDGAMVLRAKI
                     DMASPNINLRDPAIYRIRHATHHNTGDRWCIYPMYTFAHPIEDALEQITHSICTLEFE
                     DQRPFYDWLLERLCEGGLLTAPPPRQYEFARLNVGHVITSKRKLRQLVEEGHVSGWDD
                     PRMPTLAGLRRRGYTPDALRLFAERSGVTKTSNAWTDYAALDAALRETLDPVAPRAMA
                     VLEPLKLVIENWAEVFGSADHLEPCHAPVHPHHPELGQRPFMLGRELWIERSDYEDTP
                     PKGFFRLFPGNKVRLKYGYVIECIGAIKNEAGGASAVSARLIPDTKSGTPGADSVKVK
                     GVITWVAAADGLPAEVRLYDRLFTEEQPDAGGRDFLSVLNPWSLTTVQAIVEPSLAGA
                     QPEDRYQFERHGFFVADRAEHVAGDRLVFNRVTGLKDSWGK"
     gene            17989..18318
                     /locus_tag="PKN51_06890"
     CDS             17989..18318
                     /locus_tag="PKN51_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84863.1"
                     /translation="MTGLLAAGTLGVTGVAQARSDVYWSVGVGSPGVAIGVGNLPPPV
                     YVAPQPVYVAPPPVYMRPRPVYVAPPPAYYAPPHVVYRPAPRHYWGDGHHHYKHRRHH
                     RHGRDWD"
     gene            complement(18419..>18673)
                     /locus_tag="PKN51_06895"
     CDS             complement(18419..>18673)
                     /locus_tag="PKN51_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNT84864.1"
                     /translation="LQAADKYVRVVTASGEHLLRTPLKDLLEQLDPAEFWQIHRASAV
                     RADAIDSVQRDEAGRLHLSLRGSDERLVVSRLYAHRFRAL"
     assembly_gap    18674..18773
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18774..19013)
                     /locus_tag="PKN51_06900"
     CDS             complement(<18774..19013)
                     /locus_tag="PKN51_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT84865.1"
                     /translation="MSTHALTALIAEDEPLLAAALQAELARAWPELRVVASVSDGISA
                     VREALALKPDLLFFDIRMPGQSGLDAAAELMDAWDE"
     gene            complement(19010..20578)
                     /locus_tag="PKN51_06905"
     CDS             complement(19010..20578)
                     /locus_tag="PKN51_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT84866.1"
                     /translation="MNWDTIVIGSGIGGLTAAAALAHCGQRVLVLEQHDSLGGMTQTF
                     ERRGYSFATGLHYLTGVTTPEGGEGRLARLLNVLGDGTLRFRPLPRQFDLVRLVHADG
                     GETRFAYGAPEADNWARLKAQHPAEAGAIDAYAARYARARRSSNIMMTLHGMPRPAAA
                     LLRALLGPWLRRRAGETLGQALAGIDDADLRALLAARGGDYGLPPAQAPLVLHALVMG
                     SYADGGAGYPVGGPARLADSLAAAVRAAKGALRTGARVTQLLAEGGRVVGVRLAEGGV
                     ERARHVVSAMGALNTVQALPAGLAPDWRDALRRHLPSHAYITLYLGLEGSAEALRALG
                     LDGANHWIYDGQALGCAPDPEALLWQSPTDTDAPALYVSFGGLNDPEQAHAPTAEVIA
                     LCDWRFFATWRDSSLGQRPPDYEATKAWIEDRLLSQFKRLFPALKNRIAYHELSTPLT
                     QAAYALAPEGAMYGLACTPARLLDPALHVRTPVPGLLLAGQDVASLGIEGAAMGGFMA
                     AATLKPALWKLMKP"
     gene            complement(20575..21633)
                     /locus_tag="PKN51_06910"
     CDS             complement(20575..21633)
                     /locus_tag="PKN51_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNT84867.1"
                     /translation="MSIAWRDVGRHFLLVVAFCCVIAVLTTGIWPGHGYAAQLGHALC
                     IGLVTWLTIEFGRYAVDERHCHPSLEGGHGWPRGWRALLLTAIGIAAGFTLGEALGRR
                     LFLGADLRSARDDQLGLLITIAAGAVASFFFHARGRAAALAAELARAERDAGEARLAL
                     LQSQLEPHMLFNTLANLRALIGVDPQAAQQMLDRLDAYLRATLAASRSAQHPLAAEFA
                     RLDDYLALMAVRMGPRLAVALDLPDALAALPVPPLLLQPLVENAIRHGLEPRIEGGRI
                     EVSARRTGDALVLTVSDSGVGFAMTEPAAGAGGFGLAQVRERIATAYGGRARVDVQSR
                     PGGGTTVRIRLPMSGATS"
BASE COUNT         3458 a         7446 c         7482 g         3149 t
ORIGIN      
        1 ggcgacttcg agtgcgtcaa ggcgatcgcc gaggtgatca aggactccac cgtgtgctcg
       61 ctctcgcgcg ccaacgaccg cgacatcgcg cgcgccgccg aggcgctgca gggcgccgcg
      121 cgcgggcgca tccacacctt catcgccacc agtccgctgc acatggagaa aaagctgcgc
      181 atgacgcccg acgaggtgtt cgagcaggca cgccttgcgg tgcgctttgc gcgcaacctg
      241 cgcgacgaca tcgagttcag cgccgaggac ggctaccgca gcgacatcga ctttctggcc
      301 cgcgtgtgtg agacggtgat caaggaaggc gccaccacca tcaacatccc ggacaccgtg
      361 ggctacgcca ttcccgagct gtacggccac ttcatcaaga ccctgcgcga gaaagtgccc
      421 aacagcgacc aggcgatctg gagcgtgcac tgccacaacg acctgggcat ggcggtcgcc
      481 aacagcctgg cgggcgtcaa gctcggcggc gcgcgccagg tcgaatgcac catcaacggc
      541 ctgggcgagc gcgccggcaa ctgctcgctc gaagaggtgg tgatggcggt caagacgcgg
      601 cgcgatcact ttgggctgga cgtcggcatc gacaccagcc agatcgtgcc ggccagccgc
      661 atggtcagcc acaccaccgg cttcgtggtg cagcccaaca aggcggtggt gggggccaac
      721 gcctttgcgc acgccagcgg catccatcag gacggcgtgc tgaaggcgcg cgacacctac
      781 gagatcatgc gcgccgaaga cgtgggctgg agcgccaaca agatcgtgct gggcaagctg
      841 tcgggccgca ccgcgttcaa gcagcgcctg caggagctcg gcgtggtgct cgaatcggag
      901 ggcgaagtca tggccgcctt cgccaagttc aaggatctgg ccgaccgcaa gagcgagatc
      961 ttcgacgagg acattctggc cctggtcagt gacgaaagcg tgaccgccga gaaagagcag
     1021 tacggcttca tctcgctggc ccagcgcagc gaaaccggcg agaccccgca tgccgagatc
     1081 gtgttcacca tcgacggccg cgaggtgcgc ggtgtgtccg acggcaacgg cccggtggac
     1141 gcctcgctga aggcgatcga aagccaggtg tcgagcggcg ccgagatggt gctctactcg
     1201 gtcaacgcca tcagcggcaa caccgaaagc cagggcgagg tcaccgtgcg tctgcaaaac
     1261 agcggccgca ttgtcaacgg cgtgggtgcc gaccccgata tcgtggtcgc ctcggccaag
     1321 gcttatctga gtgccctcaa taagttacac agcaaagctg accgagttgc tgcgcaaggc
     1381 tagggttatc cccaggaaat ccttcgcaga atgaggaatg actcgcaagt cgatgatatt
     1441 gcgctccttt tttcacttct gtacacttgc gccgcaaacc ggacaggcat tgaggaagcg
     1501 tgatggcggc agcggcgaca ggcaggcggg tggtttttct gacgaagcat ttcttcgtac
     1561 tgggtatcgc tgccctgctg agcctgtcgg cgctgtccac ggcatcggcg gcaccggcca
     1621 agaagagcgt caaagccgcc gtggcgcaca agtccgccag cgcgcccgcc gcccgcagcg
     1681 gccggccagc cttggcgcga caggggcgtg aaacgcgacg caccgccgcc cagccgtcgg
     1741 gtcgcaccac ggcgcgcgac acgcgcggcg cccgtctgac gctggccagg caggaatccc
     1801 gtcgcaccag tggcctgagc gcgcgccaga cgcgcctgtc cctgcgcggc gccagcacat
     1861 cgcaggtcgt gcaggcgcgg gcgccgcgcc tgtccttcgg ccagatggcc ggcctgcacg
     1921 cggtggacga cccgctggat ctgaagtcca gcgtggccct ggtgctcgac caggacacgc
     1981 aggaggtgct gctgagcaaa aacgatcacg cggtgctgcc gatcgcttcg ctgaccaagc
     2041 tgatgacggg cctggtggtg gccgatgccc gcctgccgct ggacgagctg gtcaccatca
     2101 ccgaggaaga catcgacacc gagaagggca gccgctcgcg cctggccgtg ggcacccagc
     2161 tcacgcgcgg cgagctgatg cacctggcgc tgatgtccag cgaaaatcgc gccgctcatg
     2221 cgctgggccg cacctatccg ggcggcctgc agcgtttcgt gggcctgatg aacgcccgcg
     2281 ccctgatgct gggcatgaag gacacgcgct acgtcgagcc gaccggcctg tccagcggca
     2341 accgttccac cgcgcacgat ctggccaccc tggtggccgc cgccgcgcgc gagccgctgc
     2401 tgcgccagct gtccacctcc accggctacg aagtcgcggt gggcagtcgt ctggtgcagt
     2461 acaacaacac caaccgcctg gtgcacgacc cggagtggca gatcggcctg cagaagaccg
     2521 gctacatctc ggaagccggt cagtgcctgg tgatgcaggc gagggtggcg ggccgcaagc
     2581 tgatcatggt gtttctcgat tccagcggca agcacagccg tctggccgac gctgaacgcg
     2641 tgcggcgctg ggtcgaggtg cagcgaccgg ccaacaccgt ggcgggcagc ccgaacgccg
     2701 tctgatagaa actcgcgttc aaattgacag atgagcagtg acctcgctgc gcttggatga
     2761 cccatgggtt ttgggtcatg cgggggcagc cggtgtcatg gggcatggtt ctgtaggcta
     2821 ggtcgtctgg gcgcgtcgtt cgtgcgcgcc gccggcatgc gcgtggcctg acggactttg
     2881 gcgccgccgc ccgatcgcta caagtttgat agctttcggc gcaagccggg cgggcgctgg
     2941 cgcatgtttt tgttctgatg gggtgctttt cagcggcgtt cgtcaaaacc cagcgccgcc
     3001 gaaatctgct gcgccgtctg tttgagcttg ggcagccagg cgtcgtccag ccggtcggcc
     3061 ggggccgaga tcgacagccc cgccaccagc ttgccctggt cgtcgtagat gccggcggcc
     3121 atgcagcgca cgcccagctc cagctcttcg ttgtcgcgcg ccacgccggc atggcgggcg
     3181 ccgatcagct cgcgctcgag ggccggcaac tgggtgatgc tgttgcgggt gtgaccggcc
     3241 aggccggtgc gcgtggcgta ggcgcgcacg cgctggggat cgtcagacgc cagaaacagc
     3301 ttgccggtgg acgtcaggtg cagcggcgcc cgcccgccga tggcgcgcac cacctgcatg
     3361 cccgagcgtt cgctgtaggc gcgctcgatg tagacgattt cgtcgccctg gcgcacgctc
     3421 agattgaccg gttgctggat cagccggtgc agctcacgca tcggcgccag cgcggcgtcg
     3481 cgcacgttca ggcgcgcctt gaccaggttg cccagctcca gcagccgcat gcccaggcgg
     3541 tagctgccgg cctccggccg gtcgacgaag cgaccgatgg tcaggtcgtt gaggatgcgg
     3601 tgcgtggtgg aggggtgcag cccggtctgc tcgctgatgt ccttgagcga catcggctcc
     3661 tcgcgcgaag caagcacgtc gatgagcgcg aacatgcgct cgatcacctg gatggtcggc
     3721 gtctggtggg cgtcgggtct ggggcggcgc atgggcgtca ggcgggtcgg ggggcacgtt
     3781 gccactggcc gtgttccagg cgttccagcg gctgaaagcg ggccttgtac atcatcttgc
     3841 ggctgccttc gatccagtag cccaggtaca catggggcaa gcccagccgg atggcctgct
     3901 cgatctgcca cagcacggca taggtgccgt agctggcgcg cggatcgggg tcgtagaaag
     3961 tgtagacggc cgacaggccg tcgcccagca catcgacgat ggccaccatc cgcagggcgc
     4021 ccggcgcgcc gtcgcggtcc gggtcgcgaa actccaccag gcgggagttg acgttgcttt
     4081 gcagcaggaa ctgggtgtac tggtcgacgc tgtcgtggtc catgccgcca ccggtgtggc
     4141 gcccggcctg atagcgcagg tacaggcggt agtgctcggg cacatagccg agcttgagca
     4201 cgcgcacctg caggtcgcgg tggcgtgcgc gggcccggcg ctgactgcgg tcgggccgga
     4261 attcacctgc ccgcacgcgc accggaacac aggcgttgca accgtcgcag taggggcggt
     4321 aggtgaacag tccgctgcgc cgaaaacccc gctgcaccag accggagtag gtgtcgttgt
     4381 tgatcaggtg gttgggggtg gcgacctgag agcgtgcctg ccggcccggc agatagctgc
     4441 acgggtaggg cgccgtggcg tagaactgca gggcctgaag cggaagatcc ttgaggtgcg
     4501 tcaccggtct gcggtcagcg gggctggggg cagcaatgcg tcccagtata cgggctcgaa
     4561 ccgccacgcc ggggccggtt catgcaccag gcgccgcacc gcgtcggcga aggcggcgcg
     4621 cggcatttcg gccgcgccca tgaaggccag atggcgcgtg ttctgctggc agtcgatcag
     4681 cggcaggccc tgggcgcggc acagggcgat cagcgccgcc agggcgatct tggagccgtc
     4741 ggtgacctgg gtgaacatgg attcgccgaa caccgcgcga ccgatcgtca ccccgtacag
     4801 gccggcgcgc agctctccgt cgatccaggt ctcgacactg aaggcgtggc cggcgcggtg
     4861 cagtgcgccg taggcgtgtc ggatctgcgg cccgatccag gtgcccgatt ggccctggcg
     4921 cgtggcgccg gcgcaggccg cgatgacggc gtcgaaggcg ctgttgatgc ggatctcgca
     4981 gcccgcgctg gcgcgaaagc gctgcaccac ccggcgcagc gaacggtgca ggcgaaaccg
     5041 atccaccgcc agcaccatgc gcgggtcggg gctccaccac aggatcggct cgccttcgct
     5101 gaaccaggga aagatggtgg ccgcgtacgc cgccagcagg ctgggcacgt ccagcgcgcc
     5161 gcccacggcg accagaccgg gcgccggcga atgggccggc caggcctgcg acagcggcgg
     5221 aaacgggtcg cccggcttca gctgcgtcag cgggggccag gcgttcatca ggcgatatcg
     5281 aagtcgtgca cgccgaattg cccgcgccgg cggtcggcga agaagcactc cagcgtgcgg
     5341 cgcacggccg tgaaggccag ctcgtcccac ggaacctcat cttcggtgaa caggtgcacc
     5401 tgggccgact cggggccggg gtcgaaatgc tcgctcagca gccgcgccag atagaacacg
     5461 tgcacctggc cgatgcgtgg aatgctcatg gcgctgaaga aggggcccat ctcgatctgg
     5521 gccccggcct cttcggcggt ctcgcgcgcg gccccctgcg ccatgctttc gtccaactcc
     5581 atgaagccgg ccggcagggt ccatttgccc cagcgcggct cgatggcgcg ctggcacagc
     5641 agcacccgct cgcccaggta gggcacggtg cccaccacca gcagtgggtt gacgtaatga
     5701 atggtgccgc aggccgggca cacggcacgc tcgcgcgtgt cgccgtcgtc gggaatgcgg
     5761 tattgcaccg gcgtgccgca ctggcggcaa tgcttgatcg gagggcgcag catggcgcac
     5821 gcagtgtagt tccaagccag acgcggtggc gtggcggtcg ctcgctacac tgagaaggtg
     5881 agaacgaacc cctcatgccc gctttgaccg ctccaacaca cccgctcggc gttgcacatg
     5941 ctcgccgtgg ctcgggctgc ggctgcgctt tgcgccttga tcggttgcgt tgggacgctc
     6001 ctctcgggca cccggctgac cttcccacct tctgaccgct catgtcagac cgctttgcct
     6061 tcgagcgggc gccgggccat ctgatccggc gcgcgcacca gatcgccgtg gccgtgttca
     6121 ccgaagaggt cggcgcgcac ggcgtcacgc cggtgcagtt cgccatcctc aatgcgctga
     6181 tgcagcagcc cggtgtggac caggtcacac tggcgcagcg ggtggcgttc gacgccgcca
     6241 ccatcggctc ggtgataggc cggctggaga aaaagggctg gctgcgacgc caggccgacg
     6301 cggccgaccg gcggcgccgg ctgctgtggc tgaccgccga gggcgagcgc gccgccctcg
     6361 ccatgatccc ctcggtgggg caggtgcagc agcgcctcct gcaggggctg gaccatgccg
     6421 agcggcagca attgcttgca cttctggaca agctggtgag gcatcatccc cgcagcctgt
     6481 cgggctgagc gggtgctgcg cccgccgtgg ccacgccagg gtgcagaaag gagtgctgtt
     6541 catgacccgt cgcatcgttg ttccctgtcg tggcgccgtg gtggccgtca ccgccggtgc
     6601 cctgtggctg gccggctgtg ccggcatgac ggaaacccag cgcagcaccg ccatcggcgc
     6661 cggcgtcggc gcggtggccg gcggtgtgat cggccatggg cgcggcgccg tcatcggtgc
     6721 cggggtgggc gcgctggggg gctacgcctg gtcccgctac atggaaggca agcgccagca
     6781 gatgcagcag gccaccgccg gcaccggggt ggaggtgacg cagacgccgg acaaccagct
     6841 caagctgcac atcccgaacg acctttcgtt cgacaccaac cgtgccgacc tgcaaccgcg
     6901 cctgcggccc atactcgacc agtttgcgca gggcctgggc cagcagcctg gcctggaggt
     6961 gttcatcgtc ggccatgccg acgccagcgg caccgacgcc atcaacgacc cgctgtcgct
     7021 ggcgcgcgcc aacagcgtgc gcgactacct gctctcgcgc ggcgtggacg cacgctcgat
     7081 ggccaccgaa ggccgcggct cgcgcgagcc gctggccagc aacgactcgc ccgccggccg
     7141 ggcgcgcaac cgccgggtcg agatcttcct ggccgagcgc gccagcgtgg ccgcggcgcc
     7201 gcccgccgcg ccgccaccgg cctacagcac cccggtcggc cagccggtgc gctgaggccc
     7261 tgcgggctgg cggcgctata gtcgttcggc atgaagctct acaactactt ccgctcgtcc
     7321 gcgtccttca gggtgcgcat cgcactgcac ctgaagggtc tggcctacga ctacctgccg
     7381 attcaccttg ccaaaggtga gcaaaggcag cccgccttcg ccgccgtgac cgccgaaggc
     7441 ctggtgccgg tgctgcagac cgatgacgag caccggctga cgcagtcgat ggcgatcatc
     7501 gaatacctgg acgaaaccca cccccagcca ccgctgctgc cggccgacgc gctgggccgc
     7561 gcccgggtgc gcgcgctggc acagatcgtc gcctgcgaga tccatccgct caacaacctg
     7621 cgcgtgctca agtacctggt gcacgacctg aaggccagcg aggacgccaa gaacgcctgg
     7681 taccgccact gggtgcgcct gggcctgcag gcctacgaag gccagctggc cacgcaaacc
     7741 gggcgctaca gccacggcga cacgccgacg ctggccgact gctgcctggt gccgcagatc
     7801 ttcaacgccc ggcgcttcga ctgcgacttg agcggactgc cgcgcaccat ggccgtgttc
     7861 gacgcctgca tgcagctgca ggcgttccag aacgcccagc cttcggcctg ccccgacgcc
     7921 gaggcctgat gccggccggt ctgcccgccg ccgccggccc ctggctgatc cccgactggc
     7981 cgctgccgcc cggcgtgcgg gcgctgttca ccctgcgtgg gcaaagcgcc gccgacggcg
     8041 cgtccgaccc gccctgggcg catctgaacc tgggcgagca tgtgggcgac gccacggccg
     8101 cggtggcggc caaccgggcc cgcctgcgcg ccgccctggg cgcgcggccg gtgttcatgc
     8161 accaggtgca cggcacccac gtgctgaccc tgacacccga ctgcggcgat ggcaccagcg
     8221 ccgatgcggc cgtcaccgac cagccgggcc tggcctgcac cgtcatggtg gccgactgcc
     8281 tgccggtgct gctgaccgac acccggggca gggcagtggc cgcggcgcac gccggttggc
     8341 gcgggctggc ggccggggtg ctggagcaga ccttcaagcg tttgaaggcc ctggcgcttg
     8401 accagcaagc gccaccagct atcaaaatgg aagcggatga atggctggcc tggctcggcc
     8461 cctgcatcgg cccgaccgct ttcgaggtgg gcgcggaggt gcacgccgcc tttctcgccg
     8521 gcgacgccgg tgccggctcc tgcttccgcc cgcgaccgga cggcaaattc ctggccgatc
     8581 tggcggcact ggcgcggcgc cggctggcgg cactgggcgt gacgcgggtg tacggcaacg
     8641 acggcggccc cgcctggtgc accgtgggac agccggcgcg ctaccactcg taccggcgcg
     8701 acgagcgcgc cctgggcggc agcggccgca tggcggcctg catctggcgc gtgtgacgcg
     8761 gccctgcgta cccgtccggc caggcctgcg ctttgccgaa caccatggcc cgcagccaat
     8821 ggccggcgta tattggcggc gtgtcagggt ggcgttacgt tcctgacgca tactgacggc
     8881 aggcttggtc aggcgcccca ccacaaagga gacttcatga acccgtccaa caacccctgg
     8941 caggcgatgt ccgccggatt ccccgagagc atggttggcg cggcattcgg gcagggtttt
     9001 gccgaaacct ggatgaaggc gtttcagcat ttccagaaca tggacctggg caagggcgcc
     9061 ggcgaggcgc cgtcgatcga cgtgccgcag ctcaagttct cgcccgaggc gctggccaaa
     9121 ctgcagcagg actacgtggc cgaggccgcc cagctgtgga accagaccct gcaggccaac
     9181 ccgccgctca aggacaagcg ttttgccggc gaggcctggg cgtccaaccc gctggcctcg
     9241 ttcacggccg ccgtgtacct gctcaacgcc cgtaccctgc tggccatggc cgatgcggtg
     9301 gaaggcgatg ccaaggccag aaaccgcgtg cgcttcgcgg tcgagcagtg gatggccgcc
     9361 gccgcgccca gcaacttcct ggccttcaac cccgacgccc agcgcctggc catcgacacc
     9421 catggcgcca gcctggccaa gggtgtggcc aacatgctgc aggacatgaa gcagggccat
     9481 gtgtcgatga ccgacgaaag cgtgttccag gtcggcaaga acgtggccac caccgaaggc
     9541 gcggtggtgt tcgagaacga tctgttccag ctcatcgaat acaagccgct gaccgacaag
     9601 gtgcatgaaa agcccttcct tctggtgccg ccctgcatca acaagttcta catcctcgat
     9661 ctgcagccca ccaactcctt catccgctac gccgtcagcc agggcatgcg caccttcgtg
     9721 gtcagctggc gcaaccccga cgagtcgctg gccaccgcca cctgggacga ctacatcgag
     9781 cgcgccatca tccaggccat ccgcacggtg caggacatca gtggtgccag gcagatcaac
     9841 gcgctgggct tttgcgtcgg cggcaccatg ctgaccacgg ggctggccgt gctggccgcg
     9901 cgcggcgaaa agccggtctc ggccgccacc ttgctgacca cgctgatcga cttcaccgac
     9961 accggcatcc tggacgtctt catcgacgag cagatggtgc gctaccgcga gatgcagatg
    10021 ggcaagggcg ggctgatgaa ggggcaggac atggccagca ccttcagctt tctgcggccc
    10081 aacgatctgg tctggaacta cgtggtcggc aactacctga agggcgagac gccgccgccg
    10141 ttcgacctgc tgtactggaa cagcgactcc accaacctgc ccggcccgtt ctacgcctgg
    10201 tacctgcgca acacctacct cgaaaacaac ctggtcaaac ccggcaaggc caccgtctgc
    10261 ggcgagaaga tcgacctgcg caagctcgac ataccgtttt acctgtacgg ctcgcgtgaa
    10321 gaccatatcg tgccgatcgg cggcgcctac ggcagcaccc aggcgctgcc gggcaagaag
    10381 cgcttcgtca tgggctcgtc gggccacatc gccggcgtca tcaacccgcc ggcggccaga
    10441 aagcgcagcc actggcggcg cgacgacggc aagttcccga aagacgtcaa cgactggatc
    10501 gccggcgcca ccgagcaccc cggcagctgg tgggacgact gggccgactg gctcaagagc
    10561 cacgccggca agcagatcgc cgcgcccaag acctacggtc gcggcacggc ctaccgcgcc
    10621 atcgagccgg cccccggccg atacgtcaag gtcaaggcct gagcgggctc gtcgaagcat
    10681 cccgattatc aaccccatcg ggctacagcg cccgtccagc aagcgcaagc agctataaac
    10741 aggagagcaa tcgcatggaa gacatcgtca tcgtttcagc cgcccgcacc gccgtgggca
    10801 agttcggcgg ctcgctggcc aaggtgccgg ccaccgagct gggcagcatc gccatccggg
    10861 ccgcgctgga gcgcgccaag gtgggcctgg accaggtcgg tgaagtcatc ctggggcagg
    10921 tgctggccgc cggctgcggc cagaacccgg cgcgtcaggc cctgatgaag gccggcatcg
    10981 ccaaggaaac accggccatg accatcaacg ccgtctgcgg ctcgggcctc aagagcgtga
    11041 tgctggccgc gcaggccgtg gccaccggcg acagcgaaat cgtggtggcc ggcggccagg
    11101 aaaacatgag cgccagcccg cacgtgctgc tgggcagccg cgacggtcag cgcatgggcg
    11161 actggaagat gatcgactcg atgatcgtcg acggcctgtg ggacgtctac aaccagtacc
    11221 acatgggcat cacggccgag aacgtcgctc ggcaggaagg catcacgcgc gacatgcagg
    11281 acgcgctggc cctggccagc cagcagaagg ccgccgccgc ccaggccgcc ggcaagttca
    11341 aggacgagat cgtgccggtc agcatcccgc agcgcaaggg cgacccggtg gtgttcgcca
    11401 ccgacgagta catcaacacc aagaccagcg ccgaggccct ggccggcctg cgtccggcct
    11461 tcgacaaggc cggcagcgtg acggccggca acgccagcgg catcaacgac ggcgccgccg
    11521 cggtggtggt gatgagcgcc cgcaaggcgg cggccctggg cctcaagccg ctggcgcgca
    11581 tcgccagcta tgccaccagc ggcctcgatc cggccaccat gggcctgggg ccggtgccgg
    11641 cctcgcagaa ggcgctgcag cgtgccggct ggaaggtggg cgacgtcgac ctgttcgagc
    11701 tcaacgaagc ctttgccgcc caggcctgcg ccgtcaataa gctgctgggc gccgatccga
    11761 ccaaggtcaa cgtcaatggc ggcgccatcg ccatcggcca cccgatcggt gccagcggct
    11821 gccgcatcct ggtgacgctg ctgcacgaaa tgcagcgcag cggcgccaag aaggggctgg
    11881 ctggcctgtg catcggcggc ggcatgggcg tggcgatggc gctggagcgc gcctgagcct
    11941 gcaggcggcg cgggcggctg gcgtaaaccc tggggttccg ccgcctgcgc tttgagttag
    12001 attcgctgct gtcggcgcag gatatacgca ggcagctatt attttcatag tcccttgagg
    12061 agacattcca tgagtcagaa agtagcatac gtcaccggcg gcatgggtgg catcggtacc
    12121 gccatctgcc atcgcctgca caaggccggc ttcaaggtca tcgccggctg cggccccacg
    12181 cgcgactacc agaagtggct ggacgagcag aaggcgctgg gcttcacctt ccacgcgagc
    12241 gtcggcaacg tcagcgactg gcagtccacc accgacgcct tcaccaaggc caaggccgag
    12301 cacggcccga tcgacgtgct ggtcaacaac gccggcatca cgcgcgaccg catgttcctc
    12361 aagatgacgc gcgaggactg ggacgcggtg atcgacacca acctcaacag catgttcaac
    12421 gtcaccaagc aggtggtgcc cgacatggtc gagcgcggct ggggtcgcat catccagatc
    12481 agctcggtca acggcgaaaa aggccaggcc ggccagacca actactcggc cgccaaggcc
    12541 ggcatgcacg gcttcaccat ggcgctggcg caggagctgg cgagcaaagg cgtcaccgtc
    12601 aacaccgtca gccccggcta catcggcacc gacatggtgc gcgccatcaa gcccgaggtg
    12661 ctggaaaaga tcgtcgccac cattccggtc aagcgcctgg gcacgccgga agaaattggc
    12721 tccatcgtgg cctggctggc gggcgaggac tcgggcttca ccaccggcgc cgacttctcc
    12781 tgcaacggtg gtctgcacat gggttgatga ccgcgcgcgg cgcgctcgac ccaccccgct
    12841 gcggcggggt tttttgtgtc tggtagggcg gtctcgggcc atccgacgcc gaaccaccgg
    12901 ggggcaccgc gtggcaggtc gcgcccggag cgctcgggca ccgtaccggc ccggcgggga
    12961 taatcgccct cccatgcctg aagccgtttc gccctggcgc ctgtcggtcg ccccgatgct
    13021 cgactggacc gacaagcact gccgctttct gcaccgcctg ctcagtcgcc gcacgcgcct
    13081 gtacaccgag atggtgacca ccggcgccct gctgcacggc gccgagcggc gccacctgcg
    13141 ctttgacgcc gtcgagcacc cgctggcgct gcagctgggt ggcagcgaag cggccgatct
    13201 ggccgcctgc gcccgcatcg gtgcgcgctg gggctatgac gagatcaacc tgaactgcgg
    13261 ctgccccagc gaacgggtgc agcgcggcgc cttcggtgcc tgcctgatgg ccgagccgca
    13321 gctggtggcc gacggcgtca aggccatggt cgatgccgtc gacgtgccag tctccgtcaa
    13381 gcaccgcatc ggcatcgacc gcgaggagag ctacgacttc gtgcgcgact tcgtcggcac
    13441 ggtggccgag gccggctgcc gcgtcttcat cgtgcatgcg cgcaacgcct ggctgcaggg
    13501 cctgagcccg aaggagaacc gcgaaatccc gccgttgcgc catgccctgg tgtatcggct
    13561 caagcgcgac ttcccgcagc tcaccatcgt gctcaacggc ggcgtcacca gcgacgcgca
    13621 gatcaccgaa catctgcagc acgtcgatgg cgtgatgctg ggccgcgagg cctaccacca
    13681 cccctggatg atgagccgct gggacgccga atttctgggc ggtgccgcct gcgcgctgac
    13741 gcaggacgag gtcgaggccg agatggtcgc ctacatggcg cgcgaactgg ccgaggacgg
    13801 cacgccctgg tcggccatcg cgcgccacat gctgggcctg caccatggcc ggcgcggcgc
    13861 ccgcctgtgg cgccaggtgt ggagcgacca ccgtctgaag gcacggccgg cggccgaggt
    13921 ggcggcgctg gcccggtcgc atctggcggc cggggcacac gcagggcagg gcgccgtggc
    13981 gggcgcctga cggcacctgg ctcgttcgcc cttcgctgtg gcagccgacc ctagaaacgg
    14041 cagttgaccg ctggtcaact tcgggcagac cgcatcgatg cggggtctgg cggctgcgat
    14101 ggccttcacc tcctgggtga gcacgctgcg cacccgcccg caccgcgctc ggcgcgccat
    14161 gcggcgttgc ttcttcttgc gcgcaggagc ccgcggcgtc gtcgcgtctt gcctggcacg
    14221 ccgatcacgg cacgggcggg cgcgtccaac tgccgtgtct gggccaagcg atggcagcgg
    14281 gctcgtgcgg cgcgctgcga tacagcatcc tgttgatggt gtttcgggct tctggcgctt
    14341 gcccagactg cgcaagcagc taccgtattg atagcatcgg gttcccggtc agcctggtga
    14401 ccgttgcagc acccgcaccg tagtccagcg ggcgctggct ctgcgtcggc gcatcagcga
    14461 tcggcccgcc agccctgcac cagcgcgccc agcgccatgc ccgccaccgc ccacagcagg
    14521 cttaagttgc cggcgcccag gccggcgatg gccgggccgg ggcacacacc ggtcagaccc
    14581 cagccgacgc cgaacagcgc cgcgccgcgc agtgtgtcgc ggttccagct gctcgggtgc
    14641 acgccgaaac tgccgcccag cagcggccgc tgcagcaggc gcggcagcag cttgtaggcc
    14701 agcagcgtca ccgccacgcc gccgcccatc accagtgcca ggcccagatc gcgcccctgc
    14761 agaaaactca gcaccacctc gggccggatc atggaagaca gcgccagccc gaagccgaac
    14821 agcgcgcccg cggccagcgt ggccagtccc ttggcgaagc tcatacgatc cacctcgtca
    14881 ccagattggc cgtgatgaag gccgtggcca tgaaggtcag caccgccagc gccgacggcc
    14941 actgcagcga acccagcccg cagatgccat ggcccgaggt acagccgttg cccagccgcg
    15001 ccccgtagcc cgccacaaaa ccgcccgcca gcagcagcca ccagggcacc tgggtgcctt
    15061 cggccacgcc ggcgccgcgc cacagccacc acgccagagc acccagaatc agccccagcg
    15121 catacagcag ccgccagccg cgcgtgtcca ccagccgcgc ctgctgaaag aacggccgcc
    15181 gcacgacata cgaccaggtg ctggaaaaca ccgtgctcat gctgccgatg cggccggtga
    15241 aaagaaacag cagcgccacg ccggcgccca gacacaggcc gcccagcagg tagtgctgcc
    15301 aacccacggg aaaaggcgtt tgcaggatgc tcatgcggtc atcgtagcaa tccgttgcgg
    15361 tagctgccca cggtgggggc gccccgtgcc ggagctggca gttttgaacc ggctgggcgc
    15421 aggcctacag ccaacgctgt cccggcgccg gcatggtttc gacggtgaac tcgggggttc
    15481 cgcccagcac cagacccagc acgatgctga gcagcgacat gaagatgctg gcccagaacg
    15541 ccgtccagaa accatccaga tgaaagccgc gcaccagcct ggccaccagc atcagcacca
    15601 gcgcgttgat gaccagcagg aacaggccga agctgagcac ggtgagcggc agcgtcagca
    15661 gcaccagcaa gggccgcacc accgcattgg ccagccccag cagcagggcg gccaccacca
    15721 gcgccccgcc gccgtcaaag cgcaggccct tgaacacgtg cgccgccacc cacagcgcga
    15781 aggcggtgat ggcccagtgc agcaggaagg gggtgaggtt ggcgagcatg tcggcggtgt
    15841 agcgatgcag gggtggatcg taaccgtgga tggtccgcca gggtggcggg ggcgaagcgc
    15901 ggcagggcgc tacttgcccc acgagtcctt caggcccgtc acccggttga acaccagcct
    15961 gtcacccgcc acatgctcgg cccgatcggc gacgaagaag ccgtggcgct cgaactgata
    16021 gcggtcttcc ggctgcgcgc ccgccagcga cggctcgacg atggcctgca cggtggtgag
    16081 tgaccatggg ttgagcacgc tgagaaagtc gcgcccgccg gcgtcgggct gttcttcggt
    16141 gaacaggcgg tcgtacaggc gcacttcggc cggcaggccg tcggccgcgg ccacccaggt
    16201 gatcacgccc ttgaccttga cgctgtcggc gccaggggtg ccggatttgg tgtcggggat
    16261 cagtctggcg cttaccgcgc ttgcgccacc agcttcattt ttaatagcgc cgatgcactc
    16321 gatgacgtaa ccgtacttca gccgcacctt gttgccgggg aacaggcgaa agaacccctt
    16381 gggcggcgtg tcctcgtagt cgctgcgctc gatccacagc tcgcggccca gcatgaacgg
    16441 gcgctggccc agctccgggt gatgcgggtg cacgggggcg tggcagggct ccaggtgatc
    16501 ggcgctgcca aacacttcgg cccagttttc gatcaccagc ttgagcggct ccagcaccgc
    16561 catggcgcgc ggcgccaccg ggtccagcgt ttcacgcagc gcggcgtcga gtgcggcgta
    16621 gtcggtccag gcgttgctgg tcttggtgac gcccgagcgc tcggcaaaca ggcgcagggc
    16681 gtcgggcgtg tagccgcgcc gccgcaggcc ggccagcgtg ggcatgcgcg ggtcgtccca
    16741 gccgctcacg tggccttctt ccaccagctg gcggagcttg cgcttgctgg tgatgacgtg
    16801 gcccacgttc aggcgcgcaa actcgtactg gcgcggcggc ggggccgtga gcaggccgcc
    16861 ttcgcacagg cgctcgagca gccagtcgta gaacggccgc tggtcttcga actccagcgt
    16921 gcagatgctg tgggtgattt gctccagcgc gtcctcgatc gggtgcgcga aggtgtacat
    16981 ggggtagatg caccagcggt caccggtgtt gtggtgcgtg gcatggcgga tgcggtagat
    17041 ggccgggtcg cgcaggttga tgttggggct ggccatgtcg atcttggcgc gcagcaccat
    17101 ggcgccgtcc gggtgttggc catcgcgcat ctcgcgaaag cgcgccaggt tctcggccgg
    17161 cgtgcggtgg cgaaacgggc tgtcggtgcc gggcgtgccg aagtcgccgc ggctggcgcg
    17221 gatctgctcg gccgactgct cgtccacata ggcgtggccg gcttcgatca ggtgctcggc
    17281 ggcgcggtac atgaagtcga agtagtcgct ggcaaagtac tcgtgcggct gtcgcacgcc
    17341 aggtcgcgcc gggtcgtcgg cgaagtggtg gtcccagccc agccagcgca ccatgtcgcg
    17401 gatggagtcg acgtattcct gctcttcctt ttcggggttg gtgtcgtcaa agcgcaggtg
    17461 gcacacgccg ccgtattcgg cggccaggct gaagttgagc cagatgctct tggcgtggcc
    17521 gatgtgcagg tagccgttcg gttcgggcgg aaagcgggtg cggatcctgg ccggatcggc
    17581 catgccggcc tggtggctgg ggccgtcgcc cggctggccg ccccagcggc gcagggcgta
    17641 agtgccggcc tgcagatcgt gttcgatggc ctggcggatg aaatggctgg gcttggcggc
    17701 ctcggcggcc gcagtgtcgt tgggggaact catggcttcg attctaggag gccgcgggat
    17761 gcgcccgccc gatcggcagc gggcgccggt ggtgggcgtc tgcgggccgc tcgccacagg
    17821 ggcctgaacg gggcctcgcg gtcttttggg ttacgtatcg atacccctgt caccgcctgg
    17881 cagtgcgccg gacggcgcgt cgctgcgtta cggtactggg catcaacatc ggtcgccgtc
    17941 acaggcggca tgagaggagt tctgatgaaa tccctgcaag gcattctgat gacgggcctg
    18001 ctggccgcgg gcacgctggg cgtgaccggc gtggcgcagg cgcgcagcga cgtgtactgg
    18061 tcggtcggcg tgggctcacc gggcgtggcc attggcgtgg gcaacttgcc gccgccggtg
    18121 tatgtggcgc cgcagcccgt gtacgtggcc ccgccgccgg tctacatgcg tccgcgcccg
    18181 gtgtacgtgg ccccgccgcc ggcgtattac gccccgcctc acgtggtgta ccgcccggcg
    18241 ccgcgccatt actggggtga cggtcaccac cattacaagc accgcaggca tcaccggcac
    18301 gggcgcgact gggactgacg ccggtggcgc ggcgccgcct ttcgattctc caagggcatc
    18361 tgtctggcac ccgctccgtg cgagcgggtt tttttgccgc acatccgcgc ggcgcgagct
    18421 acaaggcgcg gaaccggtgc gcgtacagcc ggctcaccac cagccgttcg tcgctacccc
    18481 gcaggctgag gtgcaggcgc ccggcctcgt cgcgctgcac gctgtcgatg gcgtcggcgc
    18541 gcacggcgct ggcgcggtgg atctgccaga actcggccgg gtcgagctgt tccagcaggt
    18601 ccttgagcgg cgtgcgcagc agatgctcgc cgctggccgt gaccacgcgc acgtatttgt
    18661 cggccgcctg cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctcgtcc
    18781 caggcgtcca tcagctcggc cgcggcgtcc agcccgctct ggcccggcat gcggatgtcg
    18841 aagaacagca ggtcgggctt gagcgccagc gcctcgcgca cggcgctgat gccatcgctc
    18901 acactcgcga ccacgcgcag ctcgggccag gcgcgcgcca gctcggcctg cagggccgcg
    18961 gccagcagcg gttcgtcctc ggcgatcagg gcggtgaggg catgggtgct catggtttca
    19021 tcagcttcca gagggcgggc ttgagcgtgg cggcggccat gaacccgccc atggccgcgc
    19081 cctcgatgcc gagcgacgcc acgtcctgcc ccgcgagcag caggccgggg acgggcgtgc
    19141 ggacgtgcag cgcagggtcg agcagtcgcg cgggcgtgca cgccaggccg tacatcgccc
    19201 cttcgggcgc cagcgcgtag gcggcctgtg tcagcggggt ggagagctcg tggtaggcaa
    19261 tgcggttctt cagcgccggg aacaggcgct tgaactggct gagcaggcgg tcctcgatcc
    19321 aggccttggt ggcctcgtag tccggcggtc gctggcccag gctggagtcg cgccaggtgg
    19381 caaagaagcg ccagtcgcac agcgcgatca cctcggccgt gggcgcgtgg gcctgctcgg
    19441 gatcgttcag gccgccgaac gatacgtaca gcgccggggc atcggtatcg gtcgggctct
    19501 gccagagcag ggcctcgggg tcgggcgcgc agccaagcgc ctggccgtcg tagatccagt
    19561 ggttggcgcc gtcaaggccc agcgcacgca aggcctcggc gctgccctcc aggcccaggt
    19621 agagcgtgat gtaggcgtgg ctgggcaggt gccggcgcag cgcgtcgcgc cagtcggggg
    19681 cgagcccagc cggcagcgcc tgcaccgtat tgagcgcccc catggccgag acgacatggc
    19741 gggcacgctc gacaccgccc tcggccagcc gcacaccgac cacgcggccg ccctcggcca
    19801 gcaactgcgt cacgcgcgcg ccggtacgca gcgccccttt ggctgcgcgc accgcggccg
    19861 ccaggctgtc ggccagccgc gccgggccgc cgaccgggta gccggcaccg ccgtcggcgt
    19921 agctgcccat gaccagcgcg tgcagcacca gcggcgcctg cgcgggcggc agaccatagt
    19981 cgccaccacg cgcggccagc agggcgcgca ggtcggcgtc gtctatgccg gcgagcgcct
    20041 gcccgagcgt ctcgcccgca cggcgccgca gccacgggcc cagcagagcg cgcaacaacg
    20101 cggcggcggg ccgcggcatg ccgtgcagcg tcatcatgat gttgctgctg cggcgcgccc
    20161 gcgcgtagcg cgcggcgtac gcgtcgatgg cgccggcctc ggcgggatgc tgcgccttca
    20221 gccgcgccca gttgtcggcc tcgggggcac cgtaggcaaa gcgcgtctca ccgccatcgg
    20281 catgcaccag gcgaaccagg tcgaattgac ggggcagtgg ccgaaagcgc aacgtgccgt
    20341 cgccgaggac gttcaacagc ctggccaatc gcccctcgcc accctcgggt gtcgtcacgc
    20401 ccgtcaggta gtgcagaccg gtggcgaagc tgtagccgcg acgctcaaag gtctgcgtca
    20461 tgccgcccag gctgtcgtgc tgctccagca ccagcacgcg ctgcccgcag tgcgccagcg
    20521 ccgcggcggc ggtcaggcca ccgatgcccg agccgatgac gatggtgtcc cagttcatga
    20581 cgtggcccct gacatcggca ggcggatgcg cacggtggtg ccgccgccgg gccgcgattg
    20641 cacatcgacc cgcgcacgcc cgccataggc ggtggcgatg cgctcgcgca cctgcgccag
    20701 gccaaagccc ccggcacccg ccgcaggctc cgtcatggca aagccgacgc cgctgtcgct
    20761 taccgtcaac accagcgcat cgcccgtgcg ccttgccgac acctcgatgc ggccgccctc
    20821 gatgcgcggc tccaggccat ggcggatggc gttctccacc agtggctgca gcagcagcgg
    20881 cggcaccggc agcgcggcca gcgcgtcggg caggtccagc gcgacggcca ggcgcgggcc
    20941 catgcgcacg gccatcagcg ccagatagtc gtccaggcgc gcgaactcgg ccgccagcgg
    21001 gtgctgcgcg ctgcgcgagg ccgcgagcgt ggcgcgcagg taggcgtcga ggcggtcgag
    21061 catctgctgc gccgcctgcg ggtccacgcc gatcagcgcg cgcaggttgg ccagggtgtt
    21121 gaacaacatg tgcggctcga gctggctttg cagcagcgcg aggcgcgcct cgccggcgtc
    21181 gcgctcggcg cgcgcgagct cggccgcgag cgccgcggcg cgtccgcgtg catgaaagaa
    21241 aaagctggcc acggcgcccg cggcgatggt gatcaacagg ccgagctggt cgtcgcgcgc
    21301 cgagcgcagg tccgcgccga gaaacaggcg ccggcccagg gcctcgccca gcgtgaagcc
    21361 cgcggcgatg ccgatcgccg tgagcagcag cgcgcgccag cccctgggcc agccgtggcc
    21421 gccctccagg ctggggtggc agtgccgctc atcgaccgcg tagcgcccga actctatggt
    21481 gagccaggtc acgagcccaa tgcacagcgc atgacccagc tgcgccgcgt agccatggcc
    21541 cggccagatg ccggtggtga gcaccgcgat cacgcagcag aacgccacga ccagcagaaa
    21601 atgccggccc acgtcgcgcc aggcgatgct catgc
//
LOCUS       DAOQTZ010000043        21190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2918_length_21190_cov_13.857393, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000043 DAOQTZ010000000
VERSION     DAOQTZ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 21190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21190
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2918_length_21190_cov_13.857393"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..169)
                     /locus_tag="PKN51_06915"
     tRNA            complement(94..169)
                     /locus_tag="PKN51_06915"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Glu,seq:ttc)
     gene            complement(194..269)
                     /locus_tag="PKN51_06920"
     tRNA            complement(194..269)
                     /locus_tag="PKN51_06920"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Ala,seq:ggc)
     gene            complement(332..1738)
                     /gene="gltX"
                     /locus_tag="PKN51_06925"
     CDS             complement(332..1738)
                     /gene="gltX"
                     /locus_tag="PKN51_06925"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNT84868.1"
                     /translation="MTEKSTPAARVRTRFAPSPTGFIHLGNIRSALYPWAFARSQGGD
                     FILRIEDTDLERSTQAAVDVILEGMAWLGLDHDEGPFYQMQRMDRYREVLAQLQAAGH
                     VYPCYMSVAELDALRERQMARKEKPRYDGTWRPERGKTLPPVPAGVQPVLRFRNPQDG
                     VVAWDDKVKGRIEVHNSELDDLVIARPDGTPTYNFCVVVDDLDMRITHVIRGDDHVNN
                     TPRQINIFRALGHEPPVYAHLPTVLNEQGEKMSKRNGAKPVTQYRDEGFLPDAMVNYL
                     ARLGWSHGDDEIFSRAQFLAWFDLEHLGRSAAQFDESKLRWVNAQHLKAMDDAALAPL
                     VAAQLHQRGVTPDERLPALCGLFKDRCDTTRALADWLMPYHTDITPPADALAAHLTDA
                     VRPAIAAFADRLQAADWSAESLGAALRQTLAEHGLKMPQLAMPVRVLVMGTAQTPSVD
                     AMLALHRRDTVLARLRAA"
     gene            complement(1793..3031)
                     /locus_tag="PKN51_06930"
     CDS             complement(1793..3031)
                     /locus_tag="PKN51_06930"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNT84869.1"
                     /translation="MTTTDPGARPLPEGLHPDTLAVRTGIAPSAYGESSEALYLTSGF
                     VQPDADTSARRFAHPEQGYTYSRTGNPSVTAFERRLAALEGAEAAIGTASGMAAILLM
                     GLGLLKSGDHVVCSRSMFGSTLRLLGQEFARFGVETSFVSQTDVAEWRAAVRPGTRLL
                     FAETPTNPLTEVCDIAALAAIAHGVGARLAVDNCFATPVLQRPLALGADLVIHSGTKF
                     LDGQGRVMAGAICGSRELIEGVFAPLMRTAGMVLAPFNAWVVHKGMETLAVRVRAQSD
                     HALTLARWLERHPAVERVFYPGLPSHPQHALAMAQQAGVGGAVLAFSVRADAPEQARA
                     RAFHVIDSTRVCSIATNLGDVKTLICHPASTSHGRLSEDQRQAAGVVQGLIRLAVGLE
                     HPGDIQDDLARGLDGCRNTT"
     gene            complement(3042..4514)
                     /gene="purF"
                     /locus_tag="PKN51_06935"
     CDS             complement(3042..4514)
                     /gene="purF"
                     /locus_tag="PKN51_06935"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNT84870.1"
                     /translation="MCGIVGVVSNAPVNQLIYDALLLLQHRGQDAAGIVTQQERKFFM
                     HKARGMVRDVFRTRNMRALPGTTGLGQVRYPTAGSADSEEEAQPFYVGAPFGLVLVHN
                     GNLTNAPALKAELFSTDHRHINTESDSEVLLGVLAHELGQATRDAELTPDAVFAAVTR
                     VHQRVRGSYAVVALIAGRGLLAFRDPNGIRPLCFGHGADGAVMVASESVALEGTGFAL
                     ARDVAPGEALYVTLDGQVHARACAPAARLSPCIFEYVYLARPDSVLDGISVYQARLRL
                     GETLAKRVVSTVPPDEIDVVIPIPESSRPSANELARLLGKPYREGFVKNRYVGRTFIM
                     PGQAVRKKSVRQKLNPIASEFAGRNVLLVDDSIVRGTTSREIVQMARDAGARKVFMAS
                     AAPPVRYPNVYGIDMPTREELVAHGRTDDQVRQLIGADALIYQDVQAMKRAVAALNPA
                     VQGFDASCFDGVYVTGDLDDADVDRLSRQRHGQPVPAVAA"
     gene            complement(4558..5046)
                     /locus_tag="PKN51_06940"
     CDS             complement(4558..5046)
                     /locus_tag="PKN51_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNT84871.1"
                     /translation="MTAIDWLALTVVVVSLLLGAWRGLLYEVLALAGWVLAFVAARWA
                     AAPVGLWLPMGESPEPLRHAVGFVLVFVATAFACGLLAALARRASRALGVRPVDRSFG
                     ALFGLLRGALLLLLVAVAVRLAALHQQPWWTESVSARWLELALLQLHPFLPESFSKWL
                     SA"
     gene            complement(5226..6149)
                     /locus_tag="PKN51_06945"
     CDS             complement(5226..6149)
                     /locus_tag="PKN51_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNT84872.1"
                     /translation="MAFFKFRQRGQPQPQPEPAGRSRGKAAATETAQESIDSVRRRAR
                     HRLVGAAVLVLLAVIGFPLLFDTQPRPVVVDAPITIPDKDKTAPLRIPAPVPASASLD
                     EREEVVSAAPPAAPAARASDKADAARPAGSAATAEAPKPDDAKARTKADDAQRAAREE
                     QARQRRDEERAKAQAQARRDADDRSKREDEARARRDQDTRAKPDDAERARALLEGRGN
                     GKDKDKTAAADGQGGRFIVQVGAFAEDTAVREARQKAERAGVRTYTQVVNTAAGPRTR
                     VRVGPFASREEAERAAATLKKAGLAGSVLAL"
     gene            complement(6161..7462)
                     /gene="folC"
                     /locus_tag="PKN51_06950"
     CDS             complement(6161..7462)
                     /gene="folC"
                     /locus_tag="PKN51_06950"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNT84873.1"
                     /translation="MKTLDDWLAHCERLHPKTIELGLERVRAVAERMALRFDCPVITV
                     AGTNGKGSTCAMLEAILQQAGYRTGLYTSPHLVRFEERCRVRGDAVDASELIAACARV
                     EGARGEIALTYFEFTTLAILDVLARAGLDAVVLEVGLGGRLDAVNLIDTDCAIITSVD
                     IDHAELLGDTREQIGLEKAGILRTGRPAIVSDPLPPQSVIDRAQAIGADLWLAGRDFN
                     YSGDRQQWAWAGRGRRYAGLAYPALRGANQLVNAAGVLAALEALRPRLAVTAQAVRRG
                     LALVELPGRFQVVPGQPALVLDVAHNPHSVAALALNLDAMGFYPTTHAVFGAMADKDL
                     AAMLTRMDPIIDRWYFTDLPLPRAARGAALQAAWQAVSQRRDAASTVCDSPRQALAAA
                     AAAATPADRIVVFGSFHTVGGVLQQGTPRLQAGQPGQDARG"
     gene            7508..7873
                     /locus_tag="PKN51_06955"
     CDS             7508..7873
                     /locus_tag="PKN51_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HNT84874.1"
                     /translation="MSQTLTLYGIPHCDRVKAARAWLAAQGVSAVFHDFKTRGVPPAR
                     LPRWIDAVGWERLLNRQGSTWRRLDAACQASVCDAASAAALLMQQPSLIRRPVVEWPD
                     GAITVGFAADDWQRRLGRA"
     gene            7896..9350
                     /gene="cls"
                     /locus_tag="PKN51_06960"
     CDS             7896..9350
                     /gene="cls"
                     /locus_tag="PKN51_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNT84875.1"
                     /translation="MHLTDPMLLGHPWTFWLGWAWTLSSVVLTTWIVLQRRAPVSTLA
                     WIMVLNLMPVLGLVVYHYFGPRRVRRQRLKRWHQKAVLMSQTDLAALGAGQPPAPLWA
                     RQHTRLIQAACGIAPSSAHRVELLASGGATLEALLREVTQARRHIHLEYYIFEPDHTG
                     TRLLQALTERARAGVQVRLLVDAIGSARLLSRHGRALLDELRAAGGELAVFHPRRLDR
                     LRPLVNLRTHRKIVVVDGHAGFVGGINLTDDENEQVRPHDAYRDTHLLIRGGAVRWLQ
                     YVFLKDWHYAEGRPPGHDAHLLPEQPPGSLPVQIVASGPDTDMEGMHRAMIDALHLAR
                     QRIWLATPYFVPTEAALMALTNAALRGVQVKLMVPERSDSRIVSAAARSYYAELQAAG
                     VLVFEYRGRVLHAKTLVVDEAYGMVGSANFDNRSFRLNFEVAAVVFDADFNHRLAAMF
                     QADLSHCHLVPMQRHTPPLQRLFESVARLGSPLL"
     gene            complement(9488..10525)
                     /gene="pyrC"
                     /locus_tag="PKN51_06965"
     CDS             complement(9488..10525)
                     /gene="pyrC"
                     /locus_tag="PKN51_06965"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNT84876.1"
                     /translation="MSTAPTLTLTRPDDWHLHLRDGAALAAVVPHTAAQFGRAIVMPN
                     LRPPVTTTAQALAYKARIAQAVPAGVAFEPLMTLYLTDATPADEMARARDAGIVALKL
                     YPAGATTNSDAGVTDIRKTYRTLEAMQRLDLKLLVHGEVTDPEVDLFDREAVFIERVL
                     APLRRDFPALKIVFEHITTREAAQYVESADARLAATITAHHLLYNRNALFLGGIRPHY
                     YCLPVLKREVHRQALVRAATSGSPKFFLGTDSAPHPAHLKEHASGCAGCYTALSAIEL
                     YAEAFEAAGALQRLEGFAAFHGADFYGLPRHRDRITLRRERWTLPEQLPFGDAVVKPL
                     RGGETLAWRLA"
     gene            complement(10557..10979)
                     /gene="rnk"
                     /locus_tag="PKN51_06970"
     CDS             complement(10557..10979)
                     /gene="rnk"
                     /locus_tag="PKN51_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNT84877.1"
                     /translation="MSPAADYRTITDLDHVRIFNLLRRQGTAGQLPAQAEALAELIDA
                     ADQVPPQQIAGDIVTMYSRLRVSDADGGHERSLTLCYPADADVAQGRISVLSPLGTAL
                     LGLRVGERARWRGADGKPGELRLLAIEFQPEASGDYTA"
     gene            11416..11997
                     /locus_tag="PKN51_06975"
     CDS             11416..11997
                     /locus_tag="PKN51_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT84878.1"
                     /translation="MSLPADSPPPQGADPTAPADAATRVPLAIEDWLTVIIMALLALI
                     TFVNVLVRYFTDASFAWTEEVSIFLMIVLAMVASSAAVARGRHIRIEYFADAGPVSRQ
                     RALARLGAALVALLFGLLAVLSARVLWDDVQYGETSPGIGVPQWWYTMWLPILSLAIM
                     LRAIGLWWRGGQPAADNPELPTSPAVRDEERAP"
     assembly_gap    12030..12129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12224..13522
                     /locus_tag="PKN51_06980"
     CDS             12224..13522
                     /locus_tag="PKN51_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNT84879.1"
                     /translation="MIATLLFTLFIVIMLLGVPVGAALGLAGAAAIALANAETAWFGL
                     LAVPQNFYAGLGKYPLLAIPMFVLVGSIFDRSGVAARLVNFATAIVGRGPGMLPLIAI
                     AVAMFMGGISGSGPATAAAVGSVMIAAMARAGYPGAYSASVVGAAAATDILIPPSVAF
                     IVYSVLVPGASVPALFAAGMVPGLLVGLALIAPAVWMARRHGMGAREADMPRPPFWRS
                     LREAVWGLAAPVVILGGMRMGWFTPTEAAVVAVFYGLFVGMVVHRTMGVRDLFPILRE
                     SGELSAVILIVVSLAGIFAFSLSTLGVIDPVTQAIVHSGLGEYGVLTLIVLLLVVAGM
                     FLDGISIFLIFVPLMWPIMQHYQWDVVWFGVLLTLMVAMGQFTPPLAVNLMVSCRIAG
                     VSMEQTVRWVLPLVLAMGVVVLAVIVWPPLALWLPRALGF"
     gene            13602..14642
                     /locus_tag="PKN51_06985"
     CDS             13602..14642
                     /locus_tag="PKN51_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNT84880.1"
                     /translation="MKLRRTLLSVAAATSAALALGLPATVQAQNYKSEYRMSLVVGTA
                     FPWGKGGEIWANKVREKTNGRINIKLYPGVSLIQGDQTREFSALRQGVIDMAIGSTIN
                     WSPQVKQLNLFSLPFLMPDYAAIDALTQGAVGKEIFKILDKAGVVPLAWGENGYRELT
                     NSKRPVRTPADLKGLKIRVVGSPLFLDTFTALGANPTQMSWADAQPAFASGAVDGQEN
                     PMSIFTAAKLHNVGQKNVTMWGYVADPLVFVVNKEIWNSWTPADREAVRQAAIEAGKE
                     EIAIARKGLAEPGQPLLKDIAGLGVNIVQLSPAERDAFVKATRPIYTKWKGQIGADLV
                     NMAEKAIAARKP"
     gene            complement(14730..15467)
                     /locus_tag="PKN51_06990"
     CDS             complement(14730..15467)
                     /locus_tag="PKN51_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNT84881.1"
                     /translation="MIQVKNVSKWYGPVQVLHDCSVDINKGDVVVVCGPSGSGKSTLI
                     KTINALEPVQKGEITVNGIKVTDPATDLPKLRSQVGMVFQHFELFPHLSVTENLTLAQ
                     IKVLGRGTDEARTRGLKMLDRVGLMAHKDKFPGQLSGGQQQRVAIARALSMDPTVMLF
                     DEPTSALDPEMVGEVLDVMVTLAKEGMTMMVVTHEMGFARKVANRVIFIDVGGHILED
                     CSKEEFFGHPENRQPRTKDFLSKILQH"
     gene            complement(15493..16161)
                     /locus_tag="PKN51_06995"
     CDS             complement(15493..16161)
                     /locus_tag="PKN51_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNT84882.1"
                     /translation="MLNLDFSIFTWSLVQNFIVKGFVFSLTLTAIATLGGVFFGTLLA
                     LMRLSGQKWLDMPAAFYVNVMRSVPLVMVILWFFLLVPLIIGRPLGAEYSAIITFIAF
                     EAAYFSEIMRAGIQSIPRGQVFAGQALGMTYKQNMTLVILPQAFRNMLPVLLTQTIIL
                     FQDTSLVYAIGAYDLLKGFETAGKNFGRPIEMYLLAAVVYFIISFSLSSVVKRIHRKI
                     AIIR"
     gene            complement(16163..16921)
                     /locus_tag="PKN51_07000"
     CDS             complement(16163..16921)
                     /locus_tag="PKN51_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNT84883.1"
                     /translation="MAMDWSVFCKDTLTGEVMPGCFGSGQDITYLNWLFTAWGWTVAV
                     SLTALVVAMVMGSIVGVIRTLPDRPGLVRLGNAWVELFRNIPLLVQIFLWYFVVPALV
                     PPMKDFPPFVLVVLALGLFTSARIAEQVRAGIQALPKGQRFAGMALGLTTPQYYRYVL
                     LPMAYRIIIPPLTSESMNIFKNSAVAFAVSIPELTQYALQVGEETARPIEVYIAVTIL
                     YAVSALIINRIFAWIEKRARVPGFVAAGTGAGGH"
     gene            complement(17034..17930)
                     /locus_tag="PKN51_07005"
     CDS             complement(17034..17930)
                     /locus_tag="PKN51_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNT84884.1"
                     /translation="MKKQLLTLAVLALAAGTASAQATDTLAKIKDRGAVNLGVRDSSG
                     LAFTLGGGKYVGFHTEMAERIIDDLGKSLGKPLKINYQVITSQNRIPLIQNGTIDFEC
                     GSTTNNKARQKDVDFAFTTYVEEVRIAVKANSGIKSIKDLNGRTVATTTGTTSVQTLR
                     KNERAGGIDFKEVMGKDHADSFLLLESGRADAFVMDGSILAANIAKSKSPKDFAIVGE
                     VLSVEPIACMLPKGDAKLKKAIDDSIVRQIKDGSLTKLYDKWFMQPIPPNNVTLNMPA
                     SESTKAAWASPNDKPMEDYAAK"
     gene            complement(18065..19924)
                     /locus_tag="PKN51_07010"
     CDS             complement(18065..19924)
                     /locus_tag="PKN51_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNT84885.1"
                     /translation="MRGGGERSLSLAPGETASRQRSDAATLRRLLPYLWQYKGRVLAA
                     IAFMVGAKLANVGVPLLLKRLVDAMTLPAGGSAALLVVPVGLLLAYGALRLTNSVFNE
                     LRELVFAKATHGAARAIALRTFEHLHGLSLRFHLERQTGGMTRDIERGVRGIESLVSF
                     ALFNLAATLVEVLLVLFILARQFDIWFAVITLTALVLYIAFTVAITQWRIQFRRQANQ
                     FDSAAHSKAIDSLLNYETVKYFNNEQFEARRYDESLEQLRRAQLKARSTLALLNSGQQ
                     LIIAVGLVAMLWRATEGVAAGRMTLGDLVMVNAFMIQIYIPLNFLGVIYREIKQNLTD
                     LDKMFTLMDKEQEVADAPGAQALHTNGPVDLRFENVHFAYDPARPILRGVSFDVPAGH
                     KVAVVGPSGAGKSTLARLLFRFYDVGPEGGRILIAGQDIRRVTQASVRRAIGIVPQDT
                     VLFNDTVAYNISYGRPDASDAEVESAARAAHIHDFIMGTPAGYATMVGERGLKLSGGE
                     KQRVAIARTLLKDPPILVFDEATSALDSHNERAIQAELKSAARGKTTLVIAHRLSTVV
                     DAHEILVMEQGQIVERGTHLQLLAARGRYARMWALQQSAEPAAAPSEPESVTD"
     gene            20074..20433
                     /locus_tag="PKN51_07015"
     CDS             20074..20433
                     /locus_tag="PKN51_07015"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNT84886.1"
                     /translation="MKVIPMPADANANGDIFGGWVMAQVDLAGAVLPARYAQGRMATV
                     AVNQFVFKQPVRVGDILSFYARMERIGRSSLTVNVGVYAERFDTQGRYIKVTEATLTY
                     VAIDDSGRPRPVPRPEM"
     gene            complement(20493..20981)
                     /locus_tag="PKN51_07020"
     CDS             complement(20493..20981)
                     /locus_tag="PKN51_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT84887.1"
                     /translation="MTRPPMEPPPIAFEPEFADGLRLMFEQQVVFNRVIGLKLLSIRP
                     DGVQGRIDMREELIGHFGHRRIHGGVISAGLDAMGGIATMAAIGARHMDEPPATRLQR
                     FGKLGTIDLRIDYLRPGIGTHFLLSAEVLRLGSRVASTRMAFHGADGKLLASGAAAYM
                     VS"
BASE COUNT         3151 a         7196 c         7421 g         3322 t
ORIGIN      
        1 ccggcgtgac aggccggtat tctaaccgac tgaactacca ctccgcgtcg gtagctgcgt
       61 tgcggctctt gcgagcccaa gtgcagccaa acttggcgac cctacgggga ttcgaacccc
      121 ggtactcacc gtgaaagggt gatgtcctag gcctctagac gatagggtca aaacctggac
      181 ttctcgacaa ccttggtgga ggtaagcggg atcgaaccgc tgacctcttg catgccatgc
      241 aagcgctctc ccagctgagc tatacccccg ttgagtttcc tcgatgggct tccccgtcgt
      301 gggtgtcgag ccttgcattg tatatcgaaa ttcaggccgc gcgcaagcgg gccagcacgg
      361 tgtcgcgccg atgcagcgcc agcatggcgt cgaccgaggg cgtttgcgcc gtacccatga
      421 ccagcacgcg caccggcatg gccagttgcg gcatcttcag gccgtgctcg gccagggtct
      481 gccggagcgc agcgccaaga ctttcagcgc tccagtcggc cgcctgcagc cggtcggcaa
      541 aggcggcgat ggccggacgc acggcgtcgg tcaggtgcgc cgccagcgca tcggccgggg
      601 gggtgatgtc ggtgtggtag ggcatcagcc agtcggccag cgcccgcgtg gtgtcgcagc
      661 ggtccttgaa caggccgcac agcgcgggca ggcgttcgtc gggcgtcacg ccacgctggt
      721 gcagttgtgc cgccaccagc ggcgccagcg ccgcgtcgtc catggccttc aggtgctggg
      781 cgttgaccca gcgcagcttg gactcgtcga actgggcggc gctgcggccc aggtgttcga
      841 ggtcgaacca ggccaggaac tgcgcacgcg agaagatctc gtcgtcgcca tggctccagc
      901 ccaggcgcgc caggtagttg accatggcgt cgggcagaaa gccctcgtcg cggtactggg
      961 tgacgggctt ggcgccgttg cgcttgctca tcttttcgcc ctgctcgttc agcaccgtgg
     1021 gcaggtgggc gtacaccggc ggctcgtggc ccagggcgcg gaagatgttg atctgccgcg
     1081 gcgtgttgtt gacgtggtca tcgccgcgga tcacgtgggt gatgcgcatg tcgaggtcgt
     1141 cgaccacgac gcagaagttg taggtggggg tgccgtccgg ccgggcgatg accaggtcgt
     1201 cgagttcgct gttgtgcacc tcgatgcggc ccttgacctt gtcgtcccag gccaccacgc
     1261 cgtcctgcgg gttgcgaaag cgcagcaccg gctgcacgcc ggcgggcacg ggcggcaggg
     1321 tcttgccgcg ctcggggcgc caggtgccgt cgtagcgggg tttttccttg cgcgccatct
     1381 ggcgctcacg cagcgcgtcg agctcggcca cgctcatgta gcaggggtag acatggccgg
     1441 cggcctgcag ctgcgccagc acttcgcggt agcggtccat gcgctgcatc tggtagaacg
     1501 gcccctcgtc gtggtcgagc ccgagccagg ccatgccttc gaggatgacg tcgaccgcgg
     1561 cctgggtgga gcgttccagg tcggtgtctt cgatgcgcag aatgaagtcg ccgccctgtg
     1621 agcgcgcaaa ggcccagggg tacagggccg agcggatgtt gcccaggtga atgaagccgg
     1681 tgggcgatgg ggcaaagcgg gtgcgtacgc gcgcagcagg ggttgatttt tcagtcatat
     1741 cttgccttgg cgcttgccca gtaagcgcaa gcagctatca tttacatagc aatcaggtgg
     1801 tgttccggca cccgtccagg ccgcgcgcca ggtcgtcctg gatgtcgccc gggtgctcca
     1861 ggccgaccgc cagccggatc aggccctgca ccacgccggc ggcctgccgc tgatcctcgc
     1921 tcaggcgccc gtgcgaggtg ctggccgggt gacagatcag cgtcttgacg tcgcccaggt
     1981 tggtggcgat gctgcacacg cgggtcgaat cgatgacgtg gaaggcccgc gcgcgtgcct
     2041 gctcgggcgc gtcggcgcgc acgctgaagg cgagcaccgc accacccacg cccgcctgct
     2101 gcgccatcgc cagcgcgtgc tgcgggtgcg agggcaggcc ggggtagaac acgcgctcga
     2161 ccgccgggtg ccgctccagc cagcgggcga gggtcagcgc gtggtcgctc tgggcgcgca
     2221 cccgcaccgc cagcgtctcc atgcccttgt gcaccaccca ggcgttgaac ggcgccagca
     2281 ccatgccggc ggtgcgcatg agcggcgcga acacgccttc gatcagctcg cgcgagccac
     2341 agatggcgcc agccatcaca cgcccctgac cgtcgaggaa cttggtgccc gaatggatca
     2401 ccagatcggc ccccagcgcc agcggccgct gcagcaccgg ggtggcaaag cagttgtcca
     2461 ccgccagccg cgcgcccacg ccatgggcga tggccgccag cgcggcgatg tcgcacacct
     2521 cggtcagcgg gttggtgggg gtttcggcga acagcaggcg ggtaccgggg cgcacggcgg
     2581 cccgccattc ggccacgtcg gtctgcgaca cgaaactggt ttcgaccccg aagcgcgcga
     2641 attcctggcc cagcagccgc agcgtggagc cgaacatgga gcgcgagcac accacgtggt
     2701 cgccgctttt cagcaggccc aagcccatca gcaggatggc ggccatgccg ctggcggtgc
     2761 cgatggcggc ttcggcgcct tccagcgcgg ccaggcggcg ctcgaaggcg gtcaccgagg
     2821 ggttgccggt gcgcgaatag gtgtagccct gctccgggtg ggcaaagcgg cgggccgagg
     2881 tgtcggcgtc cggctgcacg aatccgctgg tcaggtacag cgcctcggag ctctcgccat
     2941 aggcgctggg cgcaatgccg gtgcgcaccg ccagggtgtc ggggtgcagg ccctcgggca
     3001 gcgggcgggc gccaggatca gttgtcgtca tgcggcatcc ctcaggcggc caccgccggc
     3061 actggctggc cgtggcgctg gcggctcagg cgatcgacgt cggcgtcgtc gaggtcaccg
     3121 gtgacgtaga cgccgtcgaa gcaggaggcg tcgaaaccct gcacggccgg gttgagcgcc
     3181 gccaccgccc gcttcatggc ttgcacgtcc tggtagatca gggcgtcggc gccgatcagc
     3241 tggcgcacct gatcgtcggt gcgaccatgc gccaccagct cttcgcgcgt gggcatgtcg
     3301 atgccgtaca cgttgggata gcgcaccggc ggcgccgcgc tggccatgaa gaccttgcgc
     3361 gccccggcgt cgcgcgccat ctgcacgatc tcgcggctgg tggtgccgcg cacgatggag
     3421 tcgtccacca gcagcacgtt gcgaccggcg aattcgctgg cgatggggtt gagcttctgg
     3481 cgcacggatt tcttgcgcac cgcctggccc ggcatgatga aggtgcggcc cacgtagcgg
     3541 ttcttgacga aaccctcgcg gtagggcttg cccagcaggc gcgccagctc gttggcgctg
     3601 gggcggctgg actcggggat cgggatcacg acgtcgatct cgtccggcgg cacggtcgac
     3661 accacgcgct tggccagcgt ttcacccagg cgcaggcgcg cctggtagac cgaaatgccg
     3721 tccagcaccg agtcgggccg cgccaggtac acgtactcga agatgcaggg cgacagccga
     3781 gccgccggtg cgcaggcgcg cgcatgcacc tgaccgtcga gcgtgacgta caaggcctcg
     3841 cccggcgcca cgtcgcgcgc cagcgcaaag cccgtgcctt ccagcgccac cgattcgctg
     3901 gccaccatga ccgccccgtc ggcgccgtgc ccgaagcaca gcggccggat gccgttcggg
     3961 tcgcggaacg ccagcaggcc gcgcccggcg atcagtgcca ccacggcgta ggagcctcgc
     4021 acgcgctggt gcacgcgggt gacggcggcg aacaccgcgt ccggggtgag ctcggcgtcg
     4081 cgcgtggcct gccccagctc gtgcgccagc acgcccagca gcacctcgga atcgctctcg
     4141 gtgttgatgt ggcggtggtc ggtggaaaac agttcggcct tgagcgccgg cgcgttggtc
     4201 aggttgccgt tgtgcaccag caccaggccg aacggggcgc cgacgtagaa gggctgcgcc
     4261 tcttcctcgc tgtcggcact gccggcggtc gggtaacgca cctggcccag cccggtggtg
     4321 ccgggcagcg cgcgcatgtt gcgggtgcgg aacacgtcgc gcaccatgcc cctggccttg
     4381 tgcatgaaga acttgcgctc ctgctgcgtg acgatgcccg cggcgtcctg cccgcgatgc
     4441 tgcagcagca gcagggcgtc gtaaatcagt tggttgacgg gtgcgttgct gacaacgccg
     4501 acgatgccgc acatggtgat gatcctgcgg aaagcaaaat gggccgaagc cgaattgtca
     4561 ggccgaaagc catttcgaga acgactcggg cagaaatgga tgcagctgca gcagggccag
     4621 ctccagccag cgggcgctga ccgactcggt ccaccagggc tgctggtgca gcgcagccag
     4681 acgcaccgcc accgccacca gcagcagcag caaggcgcca cgcagcaggc cgaacagcgc
     4741 gccgaaactg cggtccaccg ggcgcacgcc cagcgcccgg ctggcgcgcc gggccagcgc
     4801 cgccagcaga ccgcaggcaa aggcggtggc gacgaacacc aggacgaagc cgacggcgtg
     4861 gcgcagcggc tcgggcgact cgcccatcgg cagccacagg ccgaccggcg ccgccgccca
     4921 gcgcgcggcc acaaaggcca gcacccagcc ggccagcgcc agcacttcgt acagcaggcc
     4981 gcgccaggcg cccagcaaca gcgacaccac caccaccgtc agcgccagcc agtcgatcgc
     5041 cgtcacggcc acccctgcgc catcaccggc ggcgggtcgt gccggcgcgc cggacgggtg
     5101 cgtcggcacg cacccggtcg agccgcacca cgccgccttg gtgccggccg cggcgagcag
     5161 cgaccacggg gcgcgggggt gttgcggcgt gcaaggcggg catgggggga cgaacgcacc
     5221 gtctctcaga gcgccagcac gctgcccgcc agcccggcct ttttcagcgt ggcggcggcg
     5281 cgctcggcct cctcgcgcga ggcaaacggg ccgacgcgca cccgggtgcg cgggccggcc
     5341 gcggtgttga ccacctgggt gtaggtgcgc acgccggcgc gctcggcctt ctggcgcgcc
     5401 tcgcgcacgg cggtgtcttc ggcgaaggca cccacctgga cgatgaagcg cccgccctgg
     5461 ccgtcggcgg cggcggtctt gtccttgtcc ttgccgttgc cccggccttc cagcagggcg
     5521 cgggcgcgct cggcgtcgtc gggtttggcg cgggtgtcct ggtcgcgcct ggcccgggcc
     5581 tcgtcttcgc gcttgctgcg gtcgtcggcg tcgcgcctgg cctgcgcttg tgccttggca
     5641 cgttcctcgt cgcggcgctg gcgcgcctgt tcctcgcgtg cggcgcgctg cgcgtcgtcc
     5701 gccttggtcc tggccttggc gtcgtcgggt ttgggtgcct cggccgtcgc tgcgctgccg
     5761 gccggccgcg cggcgtcggc cttgtcgctg gcacgcgcgg cgggggcggc cggcggggcg
     5821 gcgctcacca cctcttcgcg ctcgtccagg ctggcgctgg cgggtaccgg cgcgggaatg
     5881 cgcaacgggg cggtcttgtc cttgtccggg atggtgatgg gcgcgtcgac cacgaccgga
     5941 cgcggctggg tgtcgaacag cagcggaaag ccgatcaccg ccagcagcac cagcacggcg
     6001 gcgccgacca ggcggtggcg cgcacgccgg cgcacgctgt cgatgctctc ctgcgcggtt
     6061 tcggtggcgg cggccttgcc acgcgagcgc ccggcgggtt cgggctgggg ctgaggctgg
     6121 ccgcgctggc gaaacttgaa gaaagccatg gattgctggg tcagccgcgg gcgtcttggc
     6181 cgggctgacc ggcttgcagg cggggcgtgc cctgctgcag cacgccaccc acggtgtgaa
     6241 acgaaccaaa gacgacgatt ctatcagcgg gggtggcggc ggcggccgcc gcggccagcg
     6301 cctggcgcgg cgagtcgcac accgtggacg ccgcatcacg gcgctggctg acggcctgcc
     6361 aggccgcctg cagggcggcg ccacgggccg cgcgcggcag cggcaggtcg gtgaagtacc
     6421 agcggtcgat gatcgggtcc atgcgcgtca gcatggccgc caggtccttg tcggccatgg
     6481 cgccgaacac ggcgtgggtg gtggggtaaa agcccatggc gtccaggttg agcgccagcg
     6541 ccgccaccga gtgcgggttg tgcgccacgt ccagcaccag cgccggctgg ccgggcacga
     6601 cctgaaagcg ccccggcagc tcgaccagcg ccagcccgcg gcgcaccgcc tgggcggtga
     6661 ccgccaggcg cggccgcaac gcctccagcg ccgccagcac gccggcggcg ttgaccaact
     6721 ggttggcgcc acgcagcgcc ggataggcca gcccggcata gcgtcgcccg cgtccggccc
     6781 aggcccattg ctggcggtcg ccgctgtagt tgaagtcgcg cccggccagc cacaggtcgg
     6841 cgccgatggc ctgggcgcgg tcgatcacgc tttgcggtgg cagcgggtcg ctgacgatgg
     6901 ccggccggcc ggtgcgcagg atgccggcct tttcaaggcc gatctgctcg cgcgtgtcgc
     6961 ccagcagttc ggcatggtcg atgtcgacgc tggtgatgat ggcgcagtcg gtgtcgatca
     7021 ggttgaccgc gtccagccgg ccgcccaggc ccacctccag aaccaccgcg tccagcccgg
     7081 cgcgcgccag cacgtccagg atggcgagcg tggtgaactc aaagtaggtc agggcaattt
     7141 cgcccctggc gccctccaca cgtgcgcagg cagctatcaa ttcggaagca tccacggcgt
     7201 cgccgcgaac ccggcagcgc tcctcgaagc gcaccagatg gggcgaggtg tagaggccgg
     7261 tgcgatagcc ggcctgctgc aggatggcct ccagcatggc gcaggtcgag cccttgccat
     7321 tggtgccggc cacggtgatc accgggcagt cgaagcgcag cgccatgcgc tcggccacag
     7381 cgcgcacgcg ctcgaggccc agctcgatgg tcttggggtg caggcgctcg cagtgcgcca
     7441 gccagtcgtc cagggtcttc atcacgggac gtgattgtca ccgaccatgg catcatgctc
     7501 gatcagcatg agccagaccc tgaccctgta cggcatcccc cactgcgacc gcgtcaaggc
     7561 ggcgcgtgcc tggctggccg cgcagggggt gagcgcggtg tttcacgact tcaagacgcg
     7621 cggcgtgccg ccggcgcgcc tgccgcgctg gatcgacgcc gtgggctggg agcgcctgct
     7681 gaaccgccag ggcagcacct ggcgccggct ggacgccgcc tgccaggcca gcgtgtgcga
     7741 cgccgccagc gccgccgcgc tgctgatgca gcagcccagc ctgatccggc gtccggtggt
     7801 cgaatggccg gacggcgcca tcacggtcgg cttcgcggcc gacgactggc agcggcgact
     7861 cggccgcgcg tgaccccgcc cttggcgccg cgcccatgca cctgaccgac cccatgctgc
     7921 tcgggcaccc ctggaccttc tggctgggct gggcctggac gctgtcgagc gtggtgctga
     7981 ccacctggat cgtgctgcag cgccgcgcgc cggtgtccac gctggcctgg atcatggtgc
     8041 tgaacctgat gccggtgctg gggctggtgg tctaccacta cttcggcccg cgccgggtgc
     8101 ggcgccagcg cctcaagcgc tggcaccaga aggcggtgct gatgtcgcag accgatctgg
     8161 cggcgctcgg cgccgggcag ccgccggcgc cgctgtgggc gcgccagcac acccggctga
     8221 tccaggccgc ctgcggcatc gcaccgtcga gcgcgcaccg cgtggagctg ctggccagcg
     8281 gcggcgccac gctggaggcg ctgctgcgcg aggtgacgca ggcgcgccgc cacattcacc
     8341 tggagtacta catcttcgaa ccggaccaca ccggcacccg cctgctgcag gcgctgaccg
     8401 agcgcgcccg cgccggcgtg caggtgcggc tgctggtgga cgccatcggc tcggccaggc
     8461 tgctgtcgcg ccacggccgc gcgctgctgg acgagctgcg cgccgcgggc ggcgagctgg
     8521 cggtgttcca cccgcgacgg ctggaccgcc tgcgcccgct ggtcaacctg cgcacccacc
     8581 gcaagatcgt ggtggtcgac ggccacgcgg gcttcgtcgg cggcatcaac ctgaccgacg
     8641 acgagaacga gcaggtgcgc ccgcacgacg cctaccgcga cacccacctg ctgatccgtg
     8701 gcggcgccgt gcgctggctg cagtatgtgt tcctcaagga ctggcactac gccgaaggtc
     8761 gcccgcccgg ccacgacgcg cacctgctgc ccgagcagcc gcccggcagc ctgccggtcc
     8821 agatcgtcgc ctccgggccc gacaccgaca tggaaggcat gcaccgcgcg atgatcgacg
     8881 cgctgcacct ggcgcgccag cgcatctggc tggccacgcc ctacttcgtg cccaccgagg
     8941 cggcgctgat ggcgctgacc aacgccgcgc tgcgcggggt gcaggtcaag ctgatggtgc
     9001 ccgagcgcag cgactcgcgc atcgtcagcg ccgccgcgcg ctcctactac gccgagctgc
     9061 aggccgccgg cgtgctggtg ttcgaatacc gcggccgcgt gctgcacgcc aagaccctgg
     9121 tggtggacga ggcctacggc atggtcggca gcgccaactt cgacaaccgc agctttcggc
     9181 tcaacttcga ggtggcggcg gtggtgttcg atgccgactt caaccaccgg ctggccgcca
     9241 tgttccaggc cgacctgtcg cactgccacc tggtgcccat gcagcgccac acgccgccgc
     9301 tgcagcgcct gttcgagtcg gtggcgcggc tgggctcgcc gctgctatga acccatggct
     9361 tcggaagctt gaaattcaag acagccaccg gctgaatcca gcaggcccac actacaaatc
     9421 atatagctgc tggcgcgctc tgggagcgcg ccagagcatg atttggccct gattgacggc
     9481 gccgcgatca ggccagccgc caggccagcg tctcgccgcc gcgcaggggc ttgaccaccg
     9541 cgtcgccgaa cggcagctgc tcgggcagcg tccagcgctc gcgccgcaac gtgatgcggt
     9601 cgcggtggcg cggcaggccg tagaagtcgg cgccgtggaa ggcggcaaag ccctccagcc
     9661 gctgcagcgc gccggcggct tcgaaggcct cggcgtacag ctcgatggcc gacagcgcgg
     9721 tgtagcagcc ggcgcagccg ctggcgtgct ccttcaggtg cgccgggtgc ggggcgctgt
     9781 cggtgccaag aaagaacttc gggctgccgc tggtggccgc gcgcaccagg gcctgacgat
     9841 gcacttcgcg cttgagcacc ggcaggcagt agtagtgcgg gcggatgccg cccagaaaaa
     9901 gggcgttgcg gttgtacagc aggtggtgcg cggtgatggt ggcggccagc cgggcgtcgg
     9961 cgctttccac gtactgcgcc gcctcgcgcg tggtgatgtg ctcgaagacg atcttcagcg
    10021 ccgggaaatc gcgccgcagc ggcgccagca cacgctcgat gaagaccgcc tcgcggtcga
    10081 acagatcgac ctcggggtcg gtcacctcgc catgcaccag cagcttcagg tccaggcgct
    10141 gcatggcctc cagcgtgcgg taggtcttgc gaatgtcggt gacgccggcg tcgctgttgg
    10201 tggtggcgcc ggccggatag agcttgagcg ccacgatgcc ggcgtcgcgc gcacgcgcca
    10261 tttcgtcggc cggggtggcg tcggtcaggt acagcgtcat cagcggctcg aaggccacgc
    10321 ccgccggcac cgcctgcgcg atgcgcgcct tgtaggccag cgcctgggcg gtggtggtga
    10381 ccggcgggcg caggttgggc atgacgatgg cgcggccgaa ctgggccgcc gtgtgcggca
    10441 ccacggccgc cagcgcggcg ccgtcgcgca ggtgcaggtg ccagtcgtcg gggcgggtga
    10501 gggtcagggt cggcgcggtg ctcatgcggc cgattgtccc accggcgcgg cccgcctcag
    10561 gcggtgtagt cgccactggc ttcgggctgg aattcgatcg ccagcaggcg cagctcgccc
    10621 ggcttgccgt cggcgccgcg ccagcgcgcc cgctcgccca cgcgcaggcc cagcagcgcc
    10681 gtgcccagcg gcgacagcac cgagatcctg ccctgcgcca cgtcggcgtc ggccggatag
    10741 cacagggtca ggctgcgctc gtggccgccg tcggcgtcgc tcacgcgcag gcgggagtac
    10801 atggtgacga tgtcgccggc gatctgctgc ggcggcacct ggtcggcggc gtcgatcagc
    10861 tcggccagcg cctcggcctg cgccggcaac tggcccgccg tgccctggcg gcgcagcagg
    10921 ttgaagatgc gcacgtggtc caggtcggtg atggtgcggt agtcggcagc gggtgacatg
    10981 gtggtctcct tgggaatcag ggtgatgagg gagcgccggc gcgcggcagc tggccggcag
    11041 gcgcggcaag ccgcgccggg cggggcagcc cgacgatccg gtggggcgct gtgctcggga
    11101 aagcggcgat agcgtgggga tcgtcgggct cagcggggtg cggccgtcag gcacgcacgg
    11161 caggccgctg gggcccgcgc cgacgatggc gtcaccccga cgcgcgcgcc gcgcgcaggc
    11221 gaacgccgac cggcccaggg ggccggcccg ccttgcgcgg ggccgcgcaa gcaacgatcg
    11281 ctcagggttt gcatggggac gatgatagcc gtccggggcg cctgaggcca ccccgcggcg
    11341 tttaacatcg accatttgta tccgccgacg cgcggggcgc gccggcgtgc cgcccgcgcc
    11401 gggcgtcctg ccttcatgtc cctgcctgcc gattccccgc cgccccaggg cgccgacccc
    11461 accgcgccag ccgacgccgc cacccgcgtg ccgctggcca tagaagactg gctgacggtg
    11521 atcatcatgg cgctgctggc gctgatcacc ttcgtcaacg tgctggtgcg ctacttcacc
    11581 gacgcctcct tcgcctggac cgaagaggtg tcgatcttcc tcatgatcgt gctggcaatg
    11641 gtcgccagtt cggccgcggt ggcgcgtggc cgccacattc gcatcgaata tttcgccgat
    11701 gccggccccg tctcgcgcca gcgcgcgctg gcgcgcctgg gcgccgccct ggtggcgctg
    11761 ctgttcggcc tgctggccgt gctgtcggcg cgcgtgctgt gggacgacgt gcagtacggc
    11821 gaaacctcgc ccggcatcgg cgtgccgcag tggtggtaca ccatgtggct gccgatcctc
    11881 agcctggcca tcatgctgcg cgccatcggc ctgtggtggc gcggcggcca gccggcggct
    11941 gacaaccccg agctgcccac ctcgcccgcc gtgcgcgacg aggagcgcgc gccatgaaca
    12001 cacctcgcac agcagaaaag cgccggagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnc tcaggccgcc gccgggccgc tcccaaggcg gcctgcggcc ccctcggggg
    12181 gcagcgcacc tcgcgcagcg ggggagcgtg ggggcctcat tccatgatcg ccaccctgct
    12241 gttcaccctg ttcatcgtca tcatgctgct gggcgtgccg gtcggcgcgg cgctggggct
    12301 ggcgggcgcg gcggcgattg cgctggccaa cgccgaaacc gcctggttcg gcctgctggc
    12361 ggtgccgcag aatttctacg ccggcctggg caagtacccg ctgctggcga tcccgatgtt
    12421 cgtgctggtg ggctcgatct tcgaccgctc gggcgtggcc gcgcggctgg tcaactttgc
    12481 caccgccatt gtcgggcgcg ggccgggcat gctgccgctg atcgccatcg cggtggcgat
    12541 gttcatgggc ggcatttcgg gctcggggcc ggccacggcg gcggccgtgg gcagcgtgat
    12601 gatcgccgcc atggcgcgcg ccggctatcc cggcgcctat tcggccagcg tggtgggggc
    12661 ggcggcggcc accgacatcc tgatcccgcc ctcggtggcc ttcatcgtct attcggtgct
    12721 ggtgccgggc gcctcggtgc cggcgctgtt tgccgccggt atggtgccgg gcctgctggt
    12781 ggggctggcg ctgatcgcac cggcggtgtg gatggcgcgc cgccacggca tgggcgcgcg
    12841 cgaggccgac atgccgcgcc cgccgttctg gcggagcctg cgcgaggcgg tgtgggggct
    12901 ggcggcgccg gtggtgatcc tgggcggcat gcgcatgggc tggttcacgc ccaccgaggc
    12961 ggccgtggtg gcggtgttct acggtctgtt cgtgggcatg gtggtgcacc gcaccatggg
    13021 cgtgcgcgat ctgtttccca tcctcaggga atcgggtgag ctgtcggcgg tgatcctgat
    13081 cgtggtgtcg ctggccggca tcttcgcctt ctcgctctcc accctgggcg tgatcgaccc
    13141 ggtcacccag gccatcgtgc attccggcct gggcgaatac ggcgtgctga cgctgatcgt
    13201 gctgctgctg gtggtggccg gcatgttcct ggacggcatc tcgatcttcc tgatcttcgt
    13261 gccgctgatg tggcccatca tgcagcacta ccagtgggac gtggtgtggt tcggcgtgct
    13321 gctgacgctg atggtggcca tgggccagtt cacgccgccg ctggcggtca acctgatggt
    13381 gtcgtgccgc atcgccggcg tcagcatgga acagacggtg cgctgggtgc tgccgctggt
    13441 gctggccatg ggcgtggtgg tgctggcggt gatcgtctgg ccgccgctcg cgctgtggct
    13501 gccccgggcg ctcggattct gagtcaaaac ggcttcaaag cttttaccaa cctgcgcaag
    13561 cagctctttt tttcatagtg aaccataaag gagaccctgt catgaaactg cgtcgaaccc
    13621 tgctgtccgt ggccgctgcc acctcggccg ccctggccct gggcctgccc gccacggtgc
    13681 aggcacagaa ctacaagagc gagtaccgca tgtcgctggt ggtgggcacc gccttcccct
    13741 ggggcaaggg cggcgagatc tgggccaaca aggtgcgcga aaaaaccaac ggccgcatca
    13801 acatcaagct ctaccccggc gtgtcgctga tccagggcga ccagacgcgc gagttctcgg
    13861 cgctgcgcca gggcgtgatc gacatggcga tcggctcgac catcaactgg tcgccccagg
    13921 tcaagcagct caacctgttc tcgctgccct tcctgatgcc cgactacgcc gccatcgacg
    13981 cgctgacgca aggcgcggtc ggcaaggaaa tcttcaagat cctcgacaag gccggcgtgg
    14041 tgccgctggc ctggggcgaa aacggctacc gcgaactgac caactccaag cgcccggtgc
    14101 gcacgccggc cgacctcaag ggcctgaaga tccgcgtggt cggctcgccg ctgttcctgg
    14161 ataccttcac cgccctgggc gccaacccga cgcagatgag ctgggccgac gcgcagccag
    14221 cgttcgccag cggcgcggtg gacgggcagg aaaaccccat gagcatcttc accgccgcca
    14281 agctgcacaa cgtcggccag aagaacgtca ccatgtgggg ttacgtggcc gacccgctgg
    14341 tgttcgtggt caacaaggaa atctggaatt cctggacccc ggccgaccgc gaagccgtgc
    14401 gccaggccgc catcgaagcc ggcaaggaag agatcgccat cgcccgcaag ggcctggccg
    14461 agcccggcca gccgctgctc aaggacatcg ccggactggg cgtcaacatc gtgcagctgt
    14521 cgccggccga gcgcgacgcc ttcgtcaagg ccacgcgccc gatctacacc aagtggaaag
    14581 gccagatcgg tgccgacctg gtgaacatgg ccgaaaaggc catcgcggcg cgcaagccgt
    14641 aagcccgtgc cgggggcacc ggcgcagccg gtgcccgccc gccgcacctt ctgcacgcgc
    14701 aaggcggcgg ggcggcgggc gcagcacgct cagtgctgca ggatcttgct caggaagtcc
    14761 ttggtgcgcg gctggcggtt ctcggggtgg ccgaagaatt cttccttgct gcagtcttcc
    14821 aggatgtggc cgccgacgtc gatgaagatc acgcggttgg ccaccttgcg ggcaaagccc
    14881 atttcgtgcg tgaccaccat catggtcatg ccttccttgg ccagcgtgac catcacgtcc
    14941 agcacctcgc ccaccatctc ggggtcgagc gccgacgtcg gttcgtcaaa cagcatgacg
    15001 gtggggtcca tcgacagcgc gcgtgcgatc gccacccgct gctgctgacc gcctgagagc
    15061 tggccgggga atttgtcctt gtgcgccatc aggcccacgc ggtcgagcat tttcaggccg
    15121 cgcgtgcggg cctcgtcggt gccgcggccc agcaccttga tctgggccag cgtcaggttc
    15181 tcggtcaccg acaggtgcgg gaacagctcg aaatgctgga acaccatgcc cacctgactg
    15241 cgcagcttgg gcagatcggt ggccgggtcg gtcaccttga tgccgttgac ggtgatctcg
    15301 cccttttgca ccggctccag cgcgttgatg gtcttgatga gggtggactt gcccgaaccg
    15361 gaggggccgc acaccaccac cacgtcgccc ttgttgatgt ccaccgagca gtcgtgcagc
    15421 acctgcaccg ggccgtacca cttggagacg ttcttcacct ggatcatggg gtcggacatg
    15481 gccgcggctc cttcagcgaa tgatggcgat cttgcggtgg atgcgcttga ccaccgacga
    15541 gagcgagaaa ctgatgatga aatagaccac cgccgccagc aggtacatct cgatcgggcg
    15601 gccgaagttc ttgcccgcgg tttcgaagcc cttgagcagg tcgtaggcgc cgatggcata
    15661 caccagcgag gtgtcctgga acaggatgat ggtctgcgtc agcagcaccg gcagcatgtt
    15721 gcggaacgcc tgcggcagga tcaccagcgt catgttctgc ttgtaggtca tgcccagcgc
    15781 ctggccggca aacacctggc cgcgcggaat cgactggatg ccggcgcgca tgatctcgct
    15841 gaagtaggcc gcctcgaacg cgatgaaggt gatgatggcc gaatactcgg cgccaagcgg
    15901 ccgcccgatg atcagcggca ccagcaggaa gaaccacagg atcaccatca ccagcggcac
    15961 ggaacgcatc acgttgacgt agaacgccgc cggcatgtcc agccatttct ggcccgacag
    16021 ccgcatcagc gccagcaggg tgccgaagaa caccccgccc agggtggcga tggccgtcag
    16081 cgtcaggctg aagacgaaac ccttgacgat gaagttctgg accagcgacc aggtgaagat
    16141 cgaaaaatcg aggttcagca tgtcagtgcc cccccgcccc ggtgccggcg gccacgaagc
    16201 cgggcacgcg ggcgcgcttt tcgatccacg cgaagatgcg gttgatgatc agcgccgaca
    16261 ccgcgtagag gatggtcacc gcgatgtaga cctcgatcgg ccgcgccgtc tcttcgccca
    16321 cctgcagggc gtactgggtc agctcgggga tcgacaccgc gaacgccacc gccgagttct
    16381 tgaagatgtt catcgactcg ctggtgagcg gcggaatgat gatgcgatag gccatgggca
    16441 gcagcacgta gcggtagtac tgcggcgtgg tcagccccag cgccatgccg gcaaaccgtt
    16501 gccccttggg cagcgcctgg atgccggcgc gcacctgctc ggcgatgcgc gccgaggtga
    16561 acaagcccag cgccagcacc accagcacga agggcgggaa gtccttcatg ggcggcacca
    16621 gcgccggcac cacgaaatac cacaggaaga tctgcaccag cagcgggatg ttgcgaaaca
    16681 gctcgaccca ggcgttgccc aggcgcacca gcccgggccg atccggcagg gtgcggatca
    16741 cgcccacgat ggagcccatc accatggcca ccaccagggc ggtcagcgac accgccaccg
    16801 tccagcccca ggccgtgaac agccagttga ggtaggtgat gtcctgcccg ctgccgaaac
    16861 agcccggcat gacctcgccg gtcagggtgt ccttgcagaa caccgaccaa tccatcgcca
    16921 ttgcaaccct ccttggaccg accgcgccgc gatcagcacc tcatataacc acaaacgccc
    16981 acctgcggac aagtgggcgt cgtggacatg gcctcaggcg tgtcgcccgt ggctcatttg
    17041 gccgcgtagt cctccatcgg cttgtcgttc gggctggccc aggcggcctt ggtgctttcc
    17101 gacgccggca tgttgagggt cacgttgttg ggcgggatcg gctgcatgaa ccatttgtcg
    17161 tacagcttgg tcagcgagcc atccttgatc tgacgcacga tgctgtcgtc gatcgccttc
    17221 ttcagcttgg cgtcgccctt gggcagcatg caggcgatcg gctccaccga caacacttcg
    17281 cccacgatgg cgaagtcctt ggggctcttc gacttggcga tgttggccgc caggatggag
    17341 ccgtccatca cgaaggcgtc ggcgcggccg gactcgagca gcaggaagct gtcggcgtgg
    17401 tccttgccca tcacctcctt gaagtcgatg ccgccggcgc gctcgttctt gcgcagcgtc
    17461 tgcacgctgg tggtgccggt ggtggtggcc accgtcctgc cgttcaggtc cttgatcgac
    17521 ttgatgccgg aattggcctt gacggcgatg cgcacttctt ccacgtaggt ggtgaaggcg
    17581 aagtcgacgt ccttctggcg cgccttgttg ttggtggtgg agccgcactc gaagtcgatg
    17641 gtgccgttct ggatcagcgg gatgcggttc tgcgaggtga tcacctggta gttgatcttc
    17701 agcggcttgc ccaggctctt gcccaggtcg tcgatgatgc gctcggccat ttcggtatgg
    17761 aagccgacgt atttgccgcc ccccagcgtg aaggccaggc cggaggagtc gcgcacaccc
    17821 aggttgacgg cgccgcggtc cttgatcttg gccagcgtgt cggtggcctg tgcagacgcc
    17881 gtgccggccg ccagggccag caccgccagg gtcagaagct gttttttcat tccggttcct
    17941 ttcagcagat gaacaaggga tccttcgcac ggaaggcggg accctctcaa cgcattctac
    18001 gtacccacgg ctacggacta accctgggtg tgtgcatcgg ctgaacggcc ggcccggttt
    18061 ctgctcaatc ggtcacgctt tcgggctcgg atggtgcggc cgccggctcg gcgctttgct
    18121 gcagcgccca catgcgggcg tagcggccac gcgcggccag cagttgcagg tgcgtgccgc
    18181 gctcgacgat ctggccctgt tccatcacca ggatttcgtg cgcgtccacc acggtggaca
    18241 ggcggtgcgc gatcaccagc gtggtcttgc cgcgcgcggc gctcttcagt tcggcctgga
    18301 tggcgcgctc gttgtgcgaa tcgagggcgc tggtggcctc gtcgaagacc aggatcggcg
    18361 ggtccttgag caaggtgcgg gcgatcgcca cgcgctgctt ttcgccgccc gacagcttca
    18421 gcccgcgctc gccgaccatg gtggcgtagc cggccggcgt gcccatgatg aagtcgtgga
    18481 tgtgggcggc tcgcgcggca ctctccacct cggcgtcact ggcgtcgggc cggccgtaac
    18541 tgatgttgta ggccacggtg tcgttgaaca gcaccgtatc ctgcggcacg atgccgatgg
    18601 cgcggcgcac gctggcctgg gtgacgcggc gaatgtcctg gccggcaatc aggatgcggc
    18661 ccccctcggg cccgacgtcg tagaagcgaa acagcagccg tgccagcgtg gacttgcccg
    18721 cccccgacgg ccccaccacc gccaccttgt gcccggccgg cacgtcaaag ctgacgccgc
    18781 gcaggatcgg gcgcgccggg tcgtaggcga agtgcacgtt ctcgaaacgc agatcgaccg
    18841 ggccgttggt gtgcagcgcc tgggcgccgg gcgcgtcggc cacctcctgc tccttgtcca
    18901 tgagggtgaa catcttgtcc aggtcggtca ggttctgctt gatctcgcgg tagatcacgc
    18961 ccaggaagtt gagcgggatg tagatctgga tcatgaaggc gttgaccatg accaggtcgc
    19021 ccagcgtcat gcggcccgcg gccacgccct cggtggcgcg ccagagcatg gccaccagcc
    19081 ccacggcgat gatgagctgc tggccgctgt tgagcagcgc cagcgtgctg cgtgccttga
    19141 gttgtgcgcg gcgcagctgc tccaggcttt cgtcgtagcg ccgcgcctcg aattgctcgt
    19201 tgttgaagta cttgacggtc tcgtagttca gcagcgaatc gatggccttg ctgtgcgcgg
    19261 ccgagtcgaa ctggttcgcc tggcgccgaa actgtatgcg ccactgcgtg atggccacgg
    19321 taaacgcgat gtagagcacc agcgccgtga gcgtgatgac ggcaaaccag atgtcgaact
    19381 ggcgcgccag gatgaacagc accagcagca cctcgaccag cgtggccgcg aggttgaaca
    19441 gcgcgaacga caccagcgac tcgatgccgc gcacgccgcg ctcgatgtcg cgcgtcatgc
    19501 cgccggtctg gcgctccagg tgaaagcgca acgacaggcc gtgcagatgc tcgaaggtgc
    19561 gcagcgcaat ggcgcgcgcc gcgccatgcg tggccttggc aaacaccagc tcgcgcagct
    19621 cgttgaacac cgagttcgtc aggcgcaacg caccataggc cagcagcagg cccacgggca
    19681 cgaccagcag cgcggcgctg ccgcccgcgg gcagcgtcat ggcatcgacc aggcgcttga
    19741 gcagcagagg cacgcccacg ttggccagct tggcgccgac catgaaggcg atggccgcga
    19801 gcacgcggcc cttgtactgc cacaaatagg gcagcaggcg gcgcagcgtg gccgcgtccg
    19861 agcgctggcg cgaggccgtc tcgccaggag caagggaaag ggagcgttcg ccgccaccgc
    19921 gcatggttca gaatcccgtc atcgccaaca tccgagccgt cgattctgcc catgtcccac
    19981 ctgtccagca cggtcaccgg ccatttgcgc cgccccaacg agcacccggt cgaggcgccg
    20041 gccgaaaccc cgccggccga tcacgagctg gtgatgaagg tgatccccat gccggccgac
    20101 gccaatgcca acggcgacat cttcggcggc tgggtgatgg cccaggtcga cctggccggc
    20161 gccgtgctgc cggcgcgcta cgcccagggc cgcatggcca cggtggcggt gaaccagttc
    20221 gtcttcaagc aaccggtgcg ggtgggcgac atcctgtcgt tctacgcccg catggagcgc
    20281 attggccgca gctcgctcac cgtcaacgtg ggcgtgtacg ccgagcgctt cgacacccag
    20341 gggcgctaca tcaaggtgac cgaggccacc ctgacctacg tggcgatcga cgacagcggg
    20401 cggccgcgcc cggtgccacg ccccgaaatg tgaatgaaac aggcctccag cgcgctcagg
    20461 acaagcgccg aaagctattg tttttatagc tttcaggaga ccatgtaggc cgccgccccg
    20521 ctggccagca gcttgccgtc ggccccatgg aaggccatgc gggtgctggc cacgcgcgag
    20581 cccaggcgca gcacctcggc cgacagcagg aagtgcgtgc ctatgcctgg ccgcaggtag
    20641 tcgatgcgca ggtcgatggt gcccagcttg ccaaagcgct gcaggcgggt ggcgggcggt
    20701 tcatccatgt ggcgcgcgcc gatcgccgcc atggtggcga tgccgcccat ggcgtccagg
    20761 ccggcgctga tcacgccgcc gtggatgcgc cggtggccaa agtgaccgat cagctcctcg
    20821 cgcatgtcga tgcgcccctg cacgccgtcg ggcctgatgc tgagcagctt caggccgatc
    20881 acgcggttga acaccacctg ctgctcgaac atcaggcgca gcccatcggc gaactcgggc
    20941 tcgaaggcga tgggcggtgg ctccatcggg ggacgggtca tgcggaactc acgggtgact
    21001 gaacctgcgc ctgacgttac cacgcccgcg gggcgacccg gcagcggtcg atgcggcgga
    21061 tttcagcatg cgctgaaatc cggcttgcgc ttttgacgaa agctgctgcg ccccgctgcg
    21121 cgggaccgca tctgacgatg cggcggattt cagcatgcgc tgaaatccgg cttgcgcttt
    21181 tgacgaaagc
//
LOCUS       DAOQTZ010000044        21116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2931_length_21116_cov_12.712217, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000044 DAOQTZ010000000
VERSION     DAOQTZ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 21116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21116
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2931_length_21116_cov_12.712217"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PKN51_07025"
     CDS             <1..722
                     /locus_tag="PKN51_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNT84888.1"
                     /translation="YTKGLFEWLRKAPWGNPTFSGVFISIIGFGFLGGITGVMMGTEQ
                     LNLIIHNTIYVPGHFHATVVVGTTLTFMALTYYLIPVLFKREMIAPKLAQWQPYLFGF
                     SMYFFCLVMMGAGTLGVSRRHWDMAFQGAALAYEWPGAAYLMMALVGIGGIAAIAGGA
                     IYIYITVGSLLWGRKLDAGASSPKFTPLARATPSVVAQSYGSSGFVAPGTFVLAMVFL
                     VAFVLYYFINWKYLSTLWGLS"
     gene            781..1698
                     /gene="cyoE"
                     /locus_tag="PKN51_07030"
     CDS             781..1698
                     /gene="cyoE"
                     /locus_tag="PKN51_07030"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNT84889.1"
                     /translation="MNRVDPSAAAMVMATRPATAGWRLVLSVFKLRIGTLIMLTAIAG
                     LAIVPDGGATPWQSLVLALSVLVASASAGAFNQYVEADSDRLMARTRTRAFASGALQR
                     GPGWLVLMAAMLAGAVGAAAWWVNLPAALFVLLGALTYAVVYTLGLKRRTPWNIVIGG
                     AAGGFAALAGAAAANPQVGPLGLLLALVLLLWTPPHFWSLAIANEAQYAAAGVPMLPV
                     VVGKARAARTVHRCTVVLVAVSLLPLAFGAGWAYGLGAVVGGLNFLRKTAALARAPHE
                     RARAMGAFFASMMQLSAIIAGVLIDAALR"
     gene            1718..2623
                     /locus_tag="PKN51_07035"
     CDS             1718..2623
                     /locus_tag="PKN51_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNT84890.1"
                     /translation="MATRLLRGCVAVGAIWLALAGGPALADGPGAAARAPGEVPSLDV
                     GEAVRLAEAAVGRTVPDFEMRDRRGKPVRLSDFRGKPLLVSFIYTGCFEICPTQTRTL
                     YESVKGLDVMLGQDQFNVVSIGFNQPFDAPEAMRAFAAQHRIQHRNWEFLSPRADQVD
                     ALTRAFGFSWVATPAGFDHVLGVTVVDADGRIHSQVLGDIVRADRLGVPLRRLLLYDQ
                     PLPALGQFENIVERVRILCTVYDEETGEYRYDYKLILVVLSGVLFFLSTLIYLGLEWR
                     NQRRQRRQLRAPCPSTPQPASGHGV"
     gene            2581..4152
                     /locus_tag="PKN51_07040"
     CDS             2581..4152
                     /locus_tag="PKN51_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT84891.1"
                     /translation="MSEHAPASIRSWGLSGWRALERLFDSAFGSGLNPLRHLGALGFL
                     ALWLLVASGIVLFILFDTSVTGAYASVEGLARLPLGLGHLLRGLHRYAADVLMLVMLL
                     HIVREWLHGHERGVRRFHWLTGVPLVAFCFVSAIGGFWLIWDQLSQYSALATAEWLDR
                     LPFLTSPLARNFLTAQAVSDRLFSLFIFIHLGVPVLLLFGLWFHIQRLAHATVLPPRA
                     LMLGLLGVLALLALARPVVSHAPAALGRVPAALQLDWLLLWLHPLTDVTSAAFTWALV
                     IGAMLLLLALPWLPQPARAAVAVVNPDHCSGCRRCLVDCPYAAITMVPHPDRRAGREL
                     AQVDADLCVGCGICAGACPSSTPFRRIEQLVTGIDMPQLPVDALRQRLRQDLSRATAA
                     QPLVVFACDHGAAAGGAAAGDVSTYSLLCTGQLPPSFVEYALRDGAAGVVVATCRAGG
                     CEFRLGQRWTLERMQRQREPHLRQHIPAERLELVAAGPGDAVELRAAVERLRVRVHGL
                     SDLPRIHHDDYTLQT"
     gene            4130..4681
                     /locus_tag="PKN51_07045"
     CDS             4130..4681
                     /locus_tag="PKN51_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84892.1"
                     /translation="MTTPSRPEPREGRSTAAPGAAQWAGQVLLYGLFALAIGVFSQWP
                     TYRPIDSDQAVIKISVTRLGQPVGECRRLSPDELAKLPPNMRDPLQCPRERSPLTMEV
                     DVGGQRVLQRVAPPSGLSKDGSASIYERLVVAAGPQQIDVHFKDDVRPGAVTYHRQAS
                     VNLVPGQVLVVDFDAEKGGITLQ"
     gene            4678..6153
                     /locus_tag="PKN51_07050"
     CDS             4678..6153
                     /locus_tag="PKN51_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNT84893.1"
                     /translation="MNPDTAWTPKNLRLPSPTVRAERVTASTPARFELPAVTAAGRSL
                     ARGWLWLALGALLGSGLFSVLLVLARTPGINAWLPAGDFFRVALVVHVDLSVLVWFVA
                     IAGLLWSLNQRPPGSPRSRLLLRAPLWLCGLGALGMGLAAFVDPGEAVMANYIPMLDS
                     AGFRAALWVFAAGALWLVLLTMARPVPIGPAVDGTGALRFGLHAGAVAAGVALLAFAW
                     SLVVVPTTLPAKAYYEILFWGGGHALQFTWTLLMLVAWLVLVQSCGGRVPLSPRIVVV
                     LFLIALVSVFVTPLAYLMHDVSSVEHRDMHTWGMRFGGGVAIAPLVLAVLLALLPMRG
                     LAPVQRPLRAAVLASMLLFIAGGVIGLTIQGSNVKIPAHYHGCIVGVTLALMGLVYRL
                     LPELGYAAPRGRLAVAQPWLYGVGQLLHIVGLMWSGGYGVQRKVAGAEQVLRSTGEIA
                     GMGLMGLGGLLAIVGGLLFALVVVRAMRAEPTAGLDRRAAP"
     gene            6150..8081
                     /locus_tag="PKN51_07055"
     CDS             6150..8081
                     /locus_tag="PKN51_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT84894.1"
                     /translation="MIGIVQRVLRAGFMGAEAVFNRAFGDRMNPFYHLGKISFFLFWI
                     IGATGLILYAFFDTSVEGAYQSVETITHSLWGLGGVLRSLHRYASDAMVLTMLLHMLR
                     YFAFDRLRGFRAFSWVTGVGLLWLTYVAGVNGFMLPWDRMAQFVTQASFEWMDWLPGF
                     KGRLIRNFILPEHVSNRLFSLLVFIHIGVSLLLLLVMWIHVQRVPKAAMHPPRPITVA
                     LSVTLLVLSLAVPVFSQGPADLHTMPLRLSFDWFLLTVFPLIQSGAMGAVWVLLVGST
                     AFGVMLPWLTLRRSAHAPRQLTIHPGPARVMARAGETLLDAGLRAELALPYDCRAGGC
                     GVCVCTVLNGKVDLGAYQDAVLTPAMRARGQALMCCAVALEDVEIEVEGVATLARDEA
                     SELRHLQARVQRLERISPTVMRVVLALPAGQTLPFTAGQYINIILEDGAKRAFSFANA
                     PADPAQPFPLPTDNLIELHVRLIEGGRFTTHVFEAMKEGDVVDFEGPIGRFTLRDSER
                     PILMIAGATGFAPIKSILEDAFRRGIRRPIELYWGVRSQEDLYLQDLLALWQERHANF
                     RYVPVLSDLAPDDPWPGRRGFVHQALLADHPDLTGYEVYACGSTRMVEAAVPDFIAHG
                     LGEQFCFSDAFLPTAGQGS"
     gene            complement(8174..8728)
                     /locus_tag="PKN51_07060"
     CDS             complement(8174..8728)
                     /locus_tag="PKN51_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT84895.1"
                     /translation="MSMLKGVHHVAYRCKNAKETVEWYKKYLDAEFVLAIAENEVPST
                     KEPDPYMHIFIDLGGGNILAFFELPTRAPMIAPSDANTPSWTQHLAIKVDSLDTMMKA
                     KARLEADGIEVIGPTDHTIFKSIYFRDPSGHRLELAVNTATPEMDKALDDVKWDMLEE
                     WDRTKKAPTHARWMHDGSGRVGPH"
     gene            complement(8770..8994)
                     /locus_tag="PKN51_07065"
     CDS             complement(8770..8994)
                     /locus_tag="PKN51_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HNT84896.1"
                     /translation="MPLNTQPNFSEAGRRYFQAYSPGDDFYEALIDTHRDLSDEQSAM
                     VNARLILLLANHIGDIGILRQAMQIAREGV"
     gene            complement(9021..10688)
                     /locus_tag="PKN51_07070"
     CDS             complement(9021..10688)
                     /locus_tag="PKN51_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT84897.1"
                     /translation="MQKTFTYPKFDFVRPPELADGKPGHYPVVVVGAGPVGLSAAIDL
                     AQLGQDVLLLDNDDTVSIGSRGVCYAKRALEVLDRMGCGDKFVDKGVSWDVGRTFLRE
                     GEVFNFNLRPEADHHRPGMINLQQYYLEEYLIERARQLPQLQMRFKNNVISVVPGADK
                     VTVRVETPDGPYSLTTDWLVVADGARSPIRTMMGLDIEGKIFMDRFLIADVVMKADFP
                     AERWFWFDPPFHPNQSVLLHKQADNVWRIDFQLGWQADPVEERKPENVIPRIKAMLGD
                     ERDFELEWVSIYTFQCRRMNSFNHGRVLFVGDAAHQVSPFGARGANSGIQDTDNLVWK
                     LKLVMDGKAPPTLLDSYTEERGFAADENIMNSTRSTDFITPKSNTSRTFRNAVLELAA
                     QHPFARALVNSGRLSVPSWLASSSLNTPDTDAFQGQMIPGAPMADAPVRTAGGDGWLL
                     HQVGNRFQALHYVDDAAALDTDTTRALGQLASHGIDVEPIVVARRGQAPAGIRTLIDG
                     KGCMAQRYDLQPGTTYLLRPDQHVAARWRTLNAASVKAAVARATGNT"
     gene            complement(10791..11753)
                     /locus_tag="PKN51_07075"
     CDS             complement(10791..11753)
                     /locus_tag="PKN51_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT84898.1"
                     /translation="MSEKKFASHADVEEKQISWDKLSDNAYAYTAEGDPNTGVIIGDD
                     GVMIIDATATPVAAQGVIRKIREITDKPIKYVVLTHYHAVRVLGASGYKNEGLEQIIA
                     SQDTWDLIVERGQQDMDSEIGRFPRLFQAVESVPGLTWPTMTFTGQMTVWLGKLEVKI
                     QQIGRGHTKGDTIVYLPSQNICFSGDLVEADAACYSGDAYLSEWPQTLERLAAMKFDK
                     LVPGRGPTLMTSEAVQKGLAYTRDYVGTLYQAAQQAVAQGKDLKATMAHARQLMDPKF
                     GHVFIYEHCLPFNVTRAHDEASGIRDPRIWTAERDRQMWHALQD"
     gene            complement(12014..12883)
                     /locus_tag="PKN51_07080"
     CDS             complement(12014..12883)
                     /locus_tag="PKN51_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNT84899.1"
                     /translation="MSEVTPPAHRPEDSTDTPRRQRSIQSIEVGMRVLDVLTEAGQAL
                     PLKEVAQRAAMPPGKAHPYLVSFMNVGLVKQHPLTGLYELGPKALQLGLAALEQLDPL
                     TEASGEAAQLAADSGLSVALVVRGNLGPTVVRLDEPRYPLHMNLRVGTVMSMLGTVTG
                     RVFAAFLPDAMVRAMVAGEQERVIGGRSGAGVARPQGGLGWLDSPEIQRTLADIRTLG
                     VGSGVGLPLPGVNTLSAPVFDGDGTMVLAISVIGPHGVFDPDPHGPIAALLRASAARV
                     SERLGARAGHSRA"
     gene            13092..13610
                     /locus_tag="PKN51_07085"
     CDS             13092..13610
                     /locus_tag="PKN51_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNT84900.1"
                     /translation="MVIDSLFARPRLVFASIAALCAAMLAFGILYLQNVVGLDPCPMC
                     IVQRYALIIVGVCCALAALSGRRALQRLWGLLAVGMAGFGAFTAARQSWLQWNPPELA
                     TCGRDLYGMIESFPLKRAIPMIFKGSGDCSAVDWTFLGGTIANWSFVWFVIFALLILL
                     TLAKTPAQASRS"
     gene            complement(13669..14943)
                     /gene="guaD"
                     /locus_tag="PKN51_07090"
     CDS             complement(13669..14943)
                     /gene="guaD"
                     /locus_tag="PKN51_07090"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNT84901.1"
                     /translation="MNAYRAELLRFDPHGAPLHDSDGLLVVGLGVDGVQRVIDAGPYR
                     SVAARHPQVPVTHWPGRLIAPGFIDMHVHFPQTDIIGSPADGLLPWLENYTFPAEARF
                     DSPAYAAEMANVFFAELLRHGVTTALTFATSHPVSVDAYFEAAQKRGLRMMGGKVLQD
                     RHSPDGLRDETEQSLLDSEALIRRWHGKDRLGYAITPRFAPTSSAAQLRGAGELAARY
                     RDTWIHSHVAENRDEVRWVRELYPQARSYLDVYRSFGLLRERAVYAHCIHLDEPDRQL
                     LRDSGAAAAVSPTSNLFLGSGFFDFAGAQRVGFLHGLASDVGGGTSFSPFHTMLAAYF
                     VGREGETKQGLSLQPSHLWWLHTGGAARALGLEGVIGTLQAGTEADFVLLNPRATPLL
                     ARKTERAQNLEELLFALIVLGDDRVVEQTVIA"
     gene            complement(14950..16257)
                     /locus_tag="PKN51_07095"
     CDS             complement(14950..16257)
                     /locus_tag="PKN51_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNT84902.1"
                     /translation="MSTSWIERLFKLEEHGTNVRTELIAGLTTFLTMAYIIFVNPAIL
                     GDAGMPKDAVFVATCLIAALGTAVMAFYANYPIALAPGMGLNAYFAYAVVLHMGFTWQ
                     AALGAVFISGCLFLFVTLTGIRGLIIAGIPQSLRYAITVGIGLFLALIALKSAGIVVG
                     SKATLVTLGDLHQPSVILAVLGFLLMVALDKLRVRGAILIGIVAVTIASFFFAGNEFR
                     GIFSAPPSIEPTFLKLDIMTALTKGLLNVVLVFFLVELFDATGTLMGVARRAGLLVPG
                     RMDRLNKSLFADSAAIFAGSLLGTSSTTAYVESASGVQAGGRTGLTALTVSVLFLAAL
                     FIAPLAGAVPAYATAPALFFVGCLMMRDLTELEWEDSTEVIPAAVTALMMPFTYSIAN
                     GLAFGFITYAAIKLLTGRVREVHWMVWLIAAVFLFKFFYIPGG"
     gene            complement(16274..17287)
                     /locus_tag="PKN51_07100"
     CDS             complement(16274..17287)
                     /locus_tag="PKN51_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT84903.1"
                     /translation="MNTKDVAPNPAPAGSTGDRGPLERIDLHLIRVLHTVLTEGSVSR
                     AALRLGMHQPAVSAALKRLRDLAGDPLLVRHGAQMVATDAGRRMIEPAASILRTAETL
                     FTEAQRFDPRTSTQRFHIAASDYLDPLFLPHLVADIQRQAPLTRVEIHALTGEADYRA
                     QLAQGEIDLVIGNWLQPPDELHMAHLFSDEVVSLVSHRHPAARRGWSEADWLEAEHIA
                     PMPNYPGARGIIDQVLDERGLQRRIAVRCAYFGLIPQMVADSLLVLTTGRQYCERYAR
                     QLPLTILPCPVAMPPLLYYMLWHTRSQHANAARWLRERTRQVISELRTGGPRHPSTPS
                     HKP"
     gene            complement(17338..19764)
                     /gene="xdhB"
                     /locus_tag="PKN51_07105"
     CDS             complement(17338..19764)
                     /gene="xdhB"
                     /locus_tag="PKN51_07105"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HNT84904.1"
                     /translation="MNHPVADPLLHAALPFADYRHNQQARLDSAAEAQAEQAGARVGI
                     SRPHESATLHVTGGATYIDDLPELAGTLHCALGLSPVAAGRLQGFDRDALAAMPGVVR
                     VLTAADIPGVNDCGSIVHDEPILCDGEIRFLGQPVFAVVAETREQARRAAALARQVLR
                     VDAAEPVLTPRQAHEKGQYVVPPMHLVRSASGLDEAGIRAAIARAPHRLSGSLDVGGQ
                     EQFYLEGQISYAIPKEGRGMHVHCSTQHPSEMQHLVAHALGVPAHAVLVECRRMGGGF
                     GGKESQSAQFACIAAVAAQALQRPVKLRLDRDDDFMVTGRRHGFWYEYEVGHDDEGRI
                     LGACLQMVSHAGHSADLSAPVMTRAICHFDNAYWLPEVAMHGYCGKTHTQSNTAFRGF
                     GGPQGAIAIEYILDSIARRLGRDPLAVRRANFYGQSERNVTPYGQTVRDNVIHELVDQ
                     LEASSDYDGRRAAIAAFNAGSPVLKRGIALTPLKFGISFNVRHFNQAGALVHVYTDGS
                     ILVNHGGTEMGQGLNTKVAQVVAHELGVGFERVRVTATDTSKVANTSATAASTGSDLN
                     GKAAQDAARQIRERLAACAAGRHGGLAAEVRFANDQVQVGGRTLDFATVVGEAYLDRV
                     QLWSDGFYATPGLSWDREKMQGHPFYYFAYGAAVSEVLVDTLTGEWKLLRADVLHDAG
                     RSLNPALDIGQVEGAFIQGMGWLTMEELVWHPKTGLLSTHAPSTYKIPTANDCPPVFN
                     VRLFEGRNAEDAIHRSKAVGEPPLLLPFSVFFAIRDAVSAAGGHRIDPPLQAPATSEA
                     ILRALQAVRA"
     gene            complement(19786..>21116)
                     /gene="xdhA"
                     /locus_tag="PKN51_07110"
     CDS             complement(19786..>21116)
                     /gene="xdhA"
                     /locus_tag="PKN51_07110"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HNT84905.1"
                     /translation="LSSVNACIQFLPTLDGKALFTVEDLQTLGGAAHDALHPVQQAMV
                     ECHGSQCGFCTPGIVMSLWNTYERHQQAGTQPTRQQLADDLSGNLCRCTGYRPILDAG
                     QRMFDLPPVALDRQAVTEALRALQREPALHYAAPQPTPAGPRTDHFHAPRTLQQLAEL
                     RLAKPQARLLAGSTDIGLWVNKQFRDVGDLIYVGHVAELRAIEARGDALWIGAAAALE
                     DAWRALVRRAPTLTDVWLRFASLPIRHAGTLGGNVANGSPIGDAPPILMALDAAVELR
                     RGERVRRLPLTELYLDYMKNAIEDGEFVQAIVVPDAAFRRQLRGYKVSKRFDCDISAL
                     CAGMAIELDAGVVRSVRLAYGGMAATVRRAAEAEAAITGQPWSEATVRTAQQALARDF
                     TPLTDMRASSAYRLQVAQNLLQRLWLETRPENPLPPAATSVFHLMPHAAA"
BASE COUNT         2858 a         6959 c         7619 g         3680 t
ORIGIN      
        1 gctacaccaa gggcctgttc gagtggctgc gcaaggcgcc ctggggcaac ccgacgttct
       61 ccggcgtgtt catctcgatc atcggtttcg gcttcctggg cggcatcacc ggcgtgatga
      121 tgggcaccga gcagctcaac ctgatcatcc acaacaccat ctatgtgcct gggcacttcc
      181 atgccacggt ggtggtgggc accacgctga ccttcatggc gctgacctac tacctgatcc
      241 cggtgctgtt caagcgcgag atgatcgcgc ccaagctggc gcagtggcag ccgtacctgt
      301 tcggcttctc gatgtatttc ttctgcctgg tgatgatggg tgcgggtacc ctgggcgtgt
      361 cgcgtcgcca ctgggacatg gcgttccagg gtgccgcgct ggcctatgaa tggccgggtg
      421 ccgcctacct gatgatggcg ctggtgggca tcggcggcat cgccgcgatc gccggcggcg
      481 ccatctacat ctacatcacc gtcggttcgc tgctgtgggg acgcaagctc gacgccggcg
      541 ccagcagccc gaagttcacc cccctggcgc gtgccacgcc ttcggtggtg gcccagtcct
      601 acgggtccag cggcttcgtg gcacccggca ccttcgtgct ggcgatggtg ttcctggtgg
      661 ccttcgtgct gtactacttc atcaactgga agtacctctc gaccctctgg ggcctgagct
      721 gatggtgggg cgtgaccctc gtccggcgtg acgagggtcg ccaacgagca ggaggcgcac
      781 atgaaccgag tcgacccgtc agccgccgcc atggttatgg cgacacggcc cgccaccgcc
      841 ggctggcggc tggtgctcag tgtgttcaag ctgcgcatcg gcacgctcat catgctcacc
      901 gccatcgccg gcctggcgat cgtgcccgac ggcggcgcaa cgccgtggca gtctctggtg
      961 ctggcgctgt cggtgctggt ggcttcggcc agcgccggcg ccttcaatca gtatgtggag
     1021 gccgacagcg accgcctgat ggcacgtacc cgcacgcggg cctttgccag cggcgccctg
     1081 cagcgcgggc cgggctggct ggtgctgatg gccgccatgc tggccggggc ggtcggcgcc
     1141 gccgcgtggt gggtcaacct gccggcggcg ctgttcgtgc tgctgggcgc gctgacctac
     1201 gccgtggtct acaccttggg tctgaagcgc cgcacgccgt ggaacatcgt catcggcggg
     1261 gccgccggcg gctttgcggc cctggcgggc gcggcggccg ccaaccctca ggtggggccg
     1321 ctgggtctgc tgctggcgct ggtgctgctg ctgtggacgc cgccgcattt ctggagcctg
     1381 gcgatcgcca acgaggcaca gtacgcagcc gccggtgtac ccatgctgcc ggtggtggtg
     1441 ggcaaggcgc gtgcggcgcg caccgtgcac cgctgcacgg ttgtgctggt ggccgtgtcg
     1501 ctgctgccgc tggcgttcgg ggcgggctgg gcctacgggc tgggggcggt cgtgggcggg
     1561 ctgaacttcc tgcgcaagac cgcagcgctg gcgcgtgccc cgcatgaacg ggccagggcg
     1621 atgggcgcgt tcttcgcttc gatgatgcaa ctgagtgcca tcatcgccgg ggtgctgatc
     1681 gatgccgcat tgcgctgagc aggctcgtcc gcagcgcgtg gccacacgcc tgctgcgcgg
     1741 ctgcgtagcc gtgggggcga tctggctggc gctggctggc gggccggcgc tggcggacgg
     1801 tccgggggcg gcggcgcggg cgcccggcga ggtgccctcg ctcgatgtgg gcgaagcggt
     1861 gcgcctggcc gaggctgccg tggggcgcac ggtgcccgac ttcgagatgc gcgatcggcg
     1921 cggcaagccg gtgcgcctgt cggactttcg cggcaagccc ttgctggtca gcttcatcta
     1981 caccgggtgc ttcgagatct gcccgacgca gacacggacg ctgtacgaat ccgtcaaggg
     2041 actggacgtg atgctgggcc aggaccagtt caacgtcgtc agcatcggct tcaatcaacc
     2101 attcgatgcg ccggaagcga tgcgcgcgtt tgccgcccag caccgcatcc agcatcgcaa
     2161 ctgggagttt ctgagtcccc gtgcggacca ggtggacgcc ttgacgcgtg cgttcggctt
     2221 cagctgggtg gccacccccg ccggcttcga tcatgtgctg ggggtcaccg tggtcgatgc
     2281 cgatgggcgc atccacagtc aggtgctggg cgacatcgtg cgggccgatc gcctgggtgt
     2341 gccgctgcgc cggctgctgc tgtacgacca gccgctgccg gcgctggggc agttcgagaa
     2401 catcgtcgag cgcgtgcgca tcctgtgcac ggtctacgac gaagaaaccg gcgaataccg
     2461 ctacgactac aagctgatcc tggtcgtgct cagcggcgtg ttgttctttc tttccacctt
     2521 gatctatctg gggctggagt ggcgcaatca gcggcgccag cggcgccagt tgcgagcgcc
     2581 atgtccgagc acgccccagc cagcatccgg tcatggggtc tgagtggctg gcgggcgctc
     2641 gagcggctgt tcgactctgc attcggttcc ggcctgaacc cgctgcggca tctgggcgcg
     2701 ctggggtttc tggcgctgtg gctgctggtg gccagcggca tcgtgctgtt catcctgttc
     2761 gacacctcgg tgacgggcgc ctacgcatcg gtcgaggggc tggctcggct gccgcttggg
     2821 ctgggccatc tgttgcgggg gctgcaccgc tatgcggctg acgtgctgat gctggtcatg
     2881 ctgctgcaca tcgtgcgcga gtggctgcat gggcatgagc gcggggtgcg ccgctttcac
     2941 tggctgaccg gcgtgcccct ggtggcgttc tgcttcgtga gcgccattgg cgggttctgg
     3001 ctgatctggg atcagctcag ccagtattcg gcgctggcga cggccgagtg gctcgatcgg
     3061 ctgcctttcc tgaccagccc gctggcgcgc aatttcctta ccgcgcaggc ggtcagcgac
     3121 cgtctgttct cgctcttcat cttcatccat ctgggcgtgc cggtgctgct gctgttcggc
     3181 ctgtggtttc acatccagcg gctggcccac gccacggtgc tgccgccgcg cgccctgatg
     3241 ctgggcctgc tgggcgtgct ggcgctgctg gcgctggccc gtccggtggt gagccatgcg
     3301 cccgcggcgc tggggcgggt gccggccgcg ttgcagctcg actggctgct gctgtggctg
     3361 catccgctca ccgacgtgac ctcggccgcc ttcacctggg cgctggtgat cggtgccatg
     3421 ctgctgctgc tggcgctgcc ctggctgccg cagccggcac gcgcggcggt ggcggtggtc
     3481 aaccccgacc actgcagcgg ctgccggcgc tgcctggtgg actgtcccta cgccgcgatc
     3541 accatggtgc cccatcccga tcggcgggcc gggcgcgagc tggcccaggt cgatgcggac
     3601 ctgtgcgtcg gctgcggcat ctgtgccggc gcctgcccgt cgtcgacgcc gtttcgccgc
     3661 atcgagcaac tggtgaccgg catcgatatg ccgcaactgc ccgtcgatgc cctgcgccag
     3721 cgcctgcgac aggacctgag cagggcgacc gccgcgcaac cgctggtggt gtttgcctgc
     3781 gaccacgggg cggcggccgg tggcgccgcg gcaggcgacg tcagcaccta cagcctgctg
     3841 tgcaccggcc aactgccgcc gtccttcgtc gaatacgcgc tgcgcgatgg tgcggccggc
     3901 gtcgtggtgg cgacctgccg ggcaggcggc tgcgagtttc gcctgggcca gcgctggacg
     3961 ctggagcgca tgcagcggca gcgcgagccg cacctgcgcc agcacatccc cgccgagcgc
     4021 ctggagctgg tggcggcggg tccgggcgat gcggtcgagc tgcgcgccgc ggtcgagcgt
     4081 ttgcgcgtgc gcgtgcatgg gctttccgat cttccgagaa tccaccacga tgactacacc
     4141 ctccagacct gaaccccgtg aaggccgctc caccgcagcg ccgggcgccg cccaatgggc
     4201 gggccaggtg ctgctgtatg gcctgttcgc gctggcgatc ggcgtgtttt ctcagtggcc
     4261 gacctaccgg ccaatcgaca gtgatcaggc ggtcatcaag atcagcgtca cgcgtctggg
     4321 tcagccggtg ggcgaatgcc ggcgcctgag tcccgacgag ctggccaagc tgccacccaa
     4381 catgcgcgat ccactgcagt gcccgcgcga gcgttcgccg ctgacgatgg aggtcgatgt
     4441 cggcggccaa cgcgtgctgc agcgcgtggc gccgccatcc ggcctgtcca aggacggctc
     4501 ggcgtcgatc tacgagcgcc tggtggtggc cgccggcccg caacagatcg acgtgcactt
     4561 caaggacgac gtgcgccccg gcgcggtgac ctaccaccgc caggccagtg tcaatctggt
     4621 gcccggacag gtgctggtgg tggactttga cgccgagaaa ggcggcatca cgctgcaatg
     4681 aaccccgata ccgcatggac cccgaagaat ctgcgcctgc cgtcacccac cgtgcgcgcc
     4741 gagcgcgtga cggcttcgac gccggcgcgc ttcgagctgc cggccgtcac cgcggccggg
     4801 cgctcgctgg cgcgtggctg gctgtggctg gcgctgggcg cgctgcttgg atctggtctg
     4861 ttttcggtgc tgctggtgct ggcgcgcacg cccggcatca atgcctggct gccggccgga
     4921 gacttctttc gtgtggcgct ggtggttcac gtcgacctgt cggtgctggt ctggttcgtg
     4981 gccatcgccg gcctgctgtg gagcctgaac cagaggccgc ccggctcgcc gcgcagccgc
     5041 ctgctgctgc gcgcgccgct gtggctgtgc ggtctgggcg cgctgggcat ggggctggcc
     5101 gcgttcgtgg accccggcga agcggtcatg gccaactaca tcccgatgct ggacagcgcc
     5161 gggtttcgcg ccgcgctgtg ggtgttcgcc gccggcgcgc tgtggctggt gctgctgacc
     5221 atggcgcgcc cggtgccgat cggtccggcg gtcgatggca cgggcgcctt gcgcttcggg
     5281 ctgcatgccg gcgcggtggc cgcgggcgtc gccctgctgg cgtttgcctg gtcgctggtg
     5341 gtggtgccga ccacgctgcc ggccaaggct tattacgaga ttctgttctg gggcggtggt
     5401 cacgcactgc agttcacctg gacgctgctg atgctggtgg cctggctggt gctggtgcag
     5461 tcctgcggcg ggcgggtgcc gctcagtccg cgcatcgtgg tggtgctgtt cctgatcgcc
     5521 ctggtgagcg tgttcgtcac gccgctggcg tatctgatgc atgacgtgag cagcgtcgag
     5581 catcgcgaca tgcatacctg gggcatgcgc ttcggtggtg gcgtggccat cgcgccgctg
     5641 gtgctggcgg tgctgctggc gctgctgccg atgcgcgggc tggcgccggt gcagcgcccg
     5701 ttgcgcgcgg cggtgctggc gtccatgctg ttgttcatcg ccggcggcgt gatcgggctg
     5761 acgattcagg gcagcaacgt caagattccg gcccactacc acggctgcat cgtgggcgtc
     5821 acgctggcgc tgatgggcct ggtgtaccgg ctgctgccgg agctgggcta cgccgcgccc
     5881 cgggggcggc tggccgtcgc ccagccctgg ctgtacgggg tggggcagtt gctgcacatc
     5941 gtcggcctga tgtggtcggg cggctatggc gtgcagcgca aggtggcggg tgccgagcag
     6001 gtgctgcgca gcaccggcga gatcgccggc atggggctga tggggctggg cgggctgctg
     6061 gcgatcgtcg gcggcctgct gtttgccctg gtggtggtgc gggcgatgcg ggccgaaccc
     6121 acggccggtc tggacaggag ggccgcgcca tgatcggcat cgtgcaacgc gtgctgcgag
     6181 ccggcttcat gggcgccgaa gcggtgttca accgcgcctt cggtgaccgc atgaacccct
     6241 tctaccacct cggcaagatt tcgttcttcc tgttctggat catcggcgcc acgggcctga
     6301 tcctgtatgc cttcttcgac accagcgtgg aaggcgccta ccagtcggtg gagaccatca
     6361 cccacagcct gtggggactg ggcggcgtgc tgcgttcgct gcaccgctac gcctcggatg
     6421 cgatggtgct gaccatgctg ctgcacatgt tgcgctactt tgccttcgac cggctgcgcg
     6481 ggttccgggc cttttcctgg gtcacagggg tcggtctgct gtggctgacc tacgtggccg
     6541 gggtgaacgg cttcatgctg ccctgggacc ggatggccca gttcgtcacc caggccagct
     6601 tcgagtggat ggactggctg ccgggcttca aggggcgcct gatccgcaac ttcatcctgc
     6661 ccgagcatgt cagcaatcgc ctgttctcgc tgctggtgtt catccacatc ggcgtgtccc
     6721 tgctgctgct gctagtgatg tggatccatg tgcagcgggt gcccaaggcg gccatgcatc
     6781 cgccccggcc gatcaccgtg gccctgtcgg tcacgctgct ggtgctgtcg ctggcggtgc
     6841 cggtgttcag ccaggggccg gccgatctgc acaccatgcc gctcaggctc agcttcgact
     6901 ggttcctgct gacggtgttt ccgctgattc agtccggtgc catgggcgcg gtctgggtgc
     6961 tgctggtcgg cagcaccgcg tttggtgtga tgctgccgtg gctgaccctg cggcgcagcg
     7021 cccatgcgcc gcggcaattg accatccacc ccggcccggc gcgcgtgatg gcgcgcgccg
     7081 gcgaaaccct gctggacgcc ggcctgcgcg ccgagctcgc cttgccctac gactgccgcg
     7141 caggcggctg cggcgtgtgt gtctgcaccg tgctcaacgg caaggtggac ctgggtgcat
     7201 accaggacgc ggtgctgacc ccggccatgc gggcgcgcgg ccaggccctg atgtgctgcg
     7261 ccgtggcgct ggaagacgtg gagatcgagg tcgaaggtgt ggccacgctg gcgcgcgacg
     7321 aagccagcga actgcgccac ctgcaggcaa gggtgcagcg gctggagcgc atttcaccca
     7381 cggtgatgcg ggtggtcctg gcgctgccgg ccggccagac gctgcctttc accgccgggc
     7441 agtacatcaa catcatcctg gaagacggtg ccaagcgggc gttttcgttc gccaacgcgc
     7501 cggccgatcc ggcgcaaccc tttccgctgc cgaccgacaa cctgatcgag ctgcacgtgc
     7561 ggttgatcga gggcgggcgc ttcaccaccc acgtgttcga agccatgaag gaaggcgacg
     7621 tggtcgactt cgaagggccg atcggccgct tcaccctgcg cgacagcgag cgcccgatcc
     7681 tgatgatcgc cggggcgacc gggttcgcac ccatcaagag cattctggaa gacgccttcc
     7741 gccgcggcat tcgccgcccg atcgagctgt actggggcgt gcgttcgcag gaggatctgt
     7801 acctgcagga cctgctggca ctgtggcagg agcgccatgc gaacttccgc tacgtgccgg
     7861 tgctgtccga tctggcgccg gacgacccct ggccgggccg gcgcgggttc gtgcaccagg
     7921 cgctgctggc cgaccacccc gatctgaccg gctacgaggt ctacgcctgc ggctccaccc
     7981 ggatggtcga ggcggcggtg cccgacttca tcgcgcatgg cctgggcgag cagttctgct
     8041 tctcggatgc cttcctgccc acggcggggc aggggagctg agcggcgcgt ggcaagcgcc
     8101 agtagctatg aaaaaaatag cggcagcgat cgggctgccg ctctcgtcgg ggtctggcgg
     8161 atcgtggatc ggctcagtgc gggccgacgc ggccgctgcc gtcgtgcatc cagcgggcgt
     8221 gggtgggggc cttcttggtg cggtcccatt cctccagcat gtcccacttc acgtcgtcga
     8281 gcgccttgtc catttcgggc gtggcggtgt tgacggccag ctccagccgg tggcccgatg
     8341 ggtcgcggaa gtagatcgac ttgaagatgg tgtggtcggt cgggccgatc acctcgatgc
     8401 cgtcggcttc cagtcgcgcc ttggccttca tcatggtgtc caggctgtcg accttgatcg
     8461 ccaggtgctg ggtccaggac ggggtgttgg cgtccgaggg cgcgatcatc ggcgcccggg
     8521 tgggcagctc gaagaaggcc aggatgttgc cgccgcccag gtcgatgaag atgtgcatgt
     8581 aggggtcggg ctccttggtc gaaggcactt cgttttcggc gatggccagc acgaactcgg
     8641 cgtccaggta cttcttgtac cactcgacgg tttccttggc gttcttgcag cgataggcca
     8701 cgtggtggac gcctttcagc atgctcatgg gagtctcctt gcttgggtca atggttgggg
     8761 gtggcgcggt tatacgcctt cgcgcgcgat ctgcatggcc tggcgcagga tgccgatgtc
     8821 gccgatgtgg ttggccagca gcaggatcag gcgcgcgttg accatggcgc tttgctcgtc
     8881 gctcaggtcg cggtgggtgt cgatcagcgc ttcgtagaag tcgtcgccag gcgagtaggc
     8941 ctgaaagtag cggcggccgg cttcgctgaa gttgggttgg gtgttcaggg gcatggtgct
     9001 tgtctcctgg ggttcggggc tcaggtgttg ccggtggcgc gtgccacggc ggccttgacg
     9061 ctggcggcgt tcagggtgcg ccagcgggcg gccacatgct ggtcggggcg cagcaggtag
     9121 gtggtgccgg gttggaggtc gtaacgctgg gccatgcagc ccttgccatc gatcagggtc
     9181 cggatgccgg ccggtgcctg gccccggcga gccaccacga tgggttcgac gtcgatgccg
     9241 tggctggcca gctgacccag cgcccgggtg gtgtccgtgt cgagcgcggc ggcgtcgtcc
     9301 acgtagtgca gcgcctggaa gcggttgccg acctggtgca gcagccagcc gtcgccgccg
     9361 gcggtccgca cgggggcgtc ggccatcggc gcgccgggga tcatctggcc ctggaaagcg
     9421 tcggtgtcgg gcgtgttcaa actggagctg gccagccagc tgggcaccga cagccgcccc
     9481 gaattgacca gcgcccgggc gaacgggtgc tgcgctgcca gctccagcac cgcgttgcgg
     9541 aaggtgcggc tggtgttgct cttgggtgtg atgaagtcgg tcgaacgggt cgagttcatg
     9601 atgttctcgt ccgccgcgaa gccgcgctct tcggtgtagc tgtccagcag ggtgggcggc
     9661 gccttgccgt ccatcaccag cttgagcttc cagaccaggt tgtcggtgtc ctggatgccg
     9721 ctgttggcgc cgcgggcgcc gaagggcgac acctggtgcg cggcgtcgcc gacgaacagc
     9781 acgcgcccgt ggttgaagct gttcatgcgc cggcactgga aggtgtagat gctgacccat
     9841 tcgagctcga aatcgcgttc gtcgcccagc atggccttga tgcgcgggat cacgttctcg
     9901 ggcttgcgct cttccaccgg gtcggcctgc cagccgagct ggaagtcgat gcgccagacg
     9961 ttgtcggcct gtttgtgcag cagcacgctc tggttggggt ggaagggcgg gtcgaaccag
    10021 aaccagcgtt cggccgggaa gtcggccttc atcaccacgt cggcgatcag gaagcggtcc
    10081 atgaagatct tgccctcgat gtccagcccc atcatggtgc ggatcgggct gcgcgcgccg
    10141 tcggccacca ccagccagtc ggtggtgagc gagtaggggc cgtcgggcgt ttccacacgc
    10201 acggtgacct tgtcggcgcc cggcaccacc gagatcacgt tgttcttgaa gcgcatctgc
    10261 aactgcggca gctggcgggc gcgctcgatc aggtattcct caaggtagta ctgctgcagg
    10321 ttgatcatgc ccgggcggtg gtggtcggcc tcggggcgca ggttgaagtt gaacacttcg
    10381 ccttcgcgca ggaaggtgcg gccgacgtcc cagctcacgc ccttgtcgac gaacttgtcg
    10441 ccgcagccca tgcggtcgag cacctccagc gcgcgcttgg catagcacac gccgcgcgag
    10501 ccgatggaga cggtgtcgtc gttgtccagc agcagcacgt cctggccgag ctgggccagg
    10561 tcgatggcgg cgctcaggcc caccgggccg gcaccgacca cgacgaccgg gtagtgaccc
    10621 ggcttgccgt cggccagctc gggcgggcgc acgaagtcga acttggggta ggtgaaggtc
    10681 ttttgcaccg tctgtctcct cggtggatgg tcttcgatga agtgataaat cagccgctga
    10741 cgcttgtatg gcgtgcgcca gcagctatca taacgatagt atttgggcga tcaatcctgc
    10801 agcgcgtgcc acatctgccg gtcgcgctcg gcggtccaga tgcgcggatc gcggatgccg
    10861 ctggcttcgt cgtgggcgcg cgtgacgttg aacggcaggc agtgctcgta gatgaagacg
    10921 tggccgaact tcgggtccat cagctggcgc gcgtgcgcca tggtggcctt caggtccttg
    10981 ccctgggcca cggcctgttg cgccgcctgg tacagggtgc cgacgtagtc gcgcgtgtag
    11041 gccaggcctt tctgcaccgc ctcggacgtc atcagggtgg ggccacggcc gggcaccagc
    11101 ttgtcgaact tcatggcggc cagccgctcc agcgtctgcg gccactccga caggtaggcg
    11161 tcgcccgagt agcaggcggc gtcggcttcg accaggtcgc ccgagaagca gatgttctgg
    11221 ctcggcaggt agacgatggt gtcgcccttg gtgtggccgc ggccgatctg ctggatcttg
    11281 acttccagct tgcccagcca cacggtcatc tggccggtga aggtcatggt cggccaggtc
    11341 aggccgggca cgctttccac cgcctggaac aggcgcggaa agcgcccgat ctcggaatcc
    11401 atgtcctgct ggccgcgctc gacgatcagg tcccaggtgt cctggctggc gatgatctgc
    11461 tccagccctt cgttcttgta gcccgacgcg cccagcacgc gcaccgcgtg gtagtgcgtc
    11521 agcaccacgt acttgatggg cttgtcggtg atctcgcgga tcttcctgat gacgccctgc
    11581 gccgccacgg gggtggcggt ggcgtcgatg atcatcacgc cgtcgtcgcc gatgatgacg
    11641 ccggtgttgg ggtcgccttc ggcggtgtag gcgtaggcgt tgtcgctcag cttgtcccaa
    11701 ctgatctgct tttcttcgac gtcggcgtgg gaggcgaatt tcttctcgga catggggtgg
    11761 tctccggtct gtgaaacgat ggcgcgattt tagctaaaga taatggattt atcaatatct
    11821 aatttgttcg caggacaacc ggtgttggcg ggcgcaccgg cgcggcgtcg ggccaggcgc
    11881 gcgcggccgg ctcgacccgg cctgagggac gggtctgtgc aagactgatg caggctcgcc
    11941 accccgcctg ccggggtgcg tagcggtcct gacgcagggc cggggcgccg ccgcggccaa
    12001 ggccggtgcc gcctcaggcg cggctgtgcc cggcgcgcgc gcccaggcgc tccgacacgc
    12061 gcgcggccga cgcgcgcaac agggccgcaa tggggccgtg cggatcgggg tcgaacacgc
    12121 catgcggccc gatcaccgaa atcgccagca ccatggtgcc gtcgccgtcg aacaccggtg
    12181 cgctcagggt gttgacgccc ggcagcggca gccccacgcc gctgccgacg ccgagcgtgc
    12241 ggatgtcggc cagcgtgcgc tggatctccg ggctgtccag ccagcccagc ccgccctgcg
    12301 gccgcgccac accggcgccg ctgcggccgc cgatcacgcg ttcctgttcg cccgccacca
    12361 tggcgcgcac catcgcgtcg ggcagaaagg cggcaaacac gcgcccggtg acggtgccca
    12421 gcatggacat cacggtgccc acgcgcaggt tcatgtgcag cgggtagcgc ggctcgtcca
    12481 gccgcaccac cgtgggtccc aggttgccgc gcaccaccag cgccaccgac aggccggagt
    12541 cggcggccag ctgcgccgct tcgccgctgg cttcggtcag cgggtcgagc tgctccagcg
    12601 ccgccaggcc gagctgcagc gccttcggcc ccagctcgta caggccggtc agcgggtgct
    12661 gcttgaccag cccgacgttc atgaagctga ccaggtacgg gtgcgccttg cccggcggca
    12721 tggcggcacg ctgcgccact tccttgagcg gcagggcctg gccggcctcg gtcagcacgt
    12781 ccagcacgcg catgccgacc tcgatcgact ggatgctgcg ctggcgccgc ggcgtgtcgg
    12841 tgctgtcttc gggccggtgg gcggggggcg tgacttcgga catggcgcca ttatccggat
    12901 cgcgcatgcg gcgccgagct tgtggataaa atgggcccca ggcgctttat gggcaagcgc
    12961 gagcagctat gaaaatcata gttctctgcg acctgccgag gcccggcccg ggcgccgctg
    13021 cggaacttgt ggcgggctgg cgcactccag cccagggccc gtactggttc agccttctct
    13081 tcccgttccc catcgtgatc gattctctgt ttgcccgtcc ccggctggtg tttgccagca
    13141 tcgccgccct ctgcgccgcc atgctggcgt tcggcatcct ttacctgcag aacgtcgtgg
    13201 ggctggaccc ttgcccgatg tgcatcgtgc agcgctacgc cctgatcatc gtcggcgtct
    13261 gctgcgcgct ggcggcgctg tcgggtcggc gggcgctgca gcgcctgtgg ggcctgctgg
    13321 cggtgggcat ggcgggattt ggcgccttca ccgcagcgcg ccagagctgg ctgcagtgga
    13381 acccgcccga gctggccacc tgcgggcgcg acctgtacgg catgatcgaa tcctttccgc
    13441 tcaagcgcgc catcccgatg atcttcaagg gcagcggcga ctgcagtgcg gtggactgga
    13501 cgtttctggg cggcaccatc gccaactggt cgttcgtctg gttcgtgatt tttgcgctgc
    13561 tgattctgct gacgctggcc aagacgccgg cgcaagcgtc ccggtcctga gccggcgcct
    13621 ctggcggcgt gcaccgggcg cgtcagcgcc tcaggggggt gcccttattc aagcaatcac
    13681 cgtctgctcg accacccggt cgtcgcccag cacgatcagg gcgaacagca gctcctccag
    13741 gttctgcgcg cgctcggtct tgcgcgccag cagcggcgtg gccctggggt tgagcagcac
    13801 gaaatcggcc tcggtgccgg cctgcagggt gccgatcacg ccctccagac ccagtgcacg
    13861 cgccgcgccg ccggtgtgca gccaccacag gtgcgacggt tgcaggctca ggccctgctt
    13921 ggtctctcct tcgcgcccga cgaaataggc cgccagcatg gtgtggaacg ggctgaagct
    13981 ggtgccaccg cccacgtcgc tggccagacc gtgcagaaac ccgacccgct gcgcccctgc
    14041 gaagtcaaag aacccgctgc ccagaaacag gttgctggtg gggctgacgg cggcggcggc
    14101 gccgctgtcg cgcagcaact ggcggtcggg ttcgtccagg tggatgcagt gcgcgtacac
    14161 cgcgcgctcg cgcagcaggc cgaagctgcg gtacacgtcc aggtacgagc gcgcctgcgg
    14221 gtacagctcg cgcacccacc tgacctcgtc gcggttttcg gccacgtggg aatggatcca
    14281 ggtgtcgcgg tagcgcgccg ccagctcgcc cgcgccgcgc agctgcgccg cgctgctggt
    14341 gggggcaaag cgtggcgtga tggcatagcc cagccggtcc ttgccgtgcc agcggcggat
    14401 cagcgcctcg ctgtcgagca ggctctgctc ggtctcgtcg cgcaggccgt cgggcgagtg
    14461 gcggtcctgc agcaccttgc cgcccatcat gcgcaggccg cgtttttgtg cagcctcgaa
    14521 ataggcgtcc accgacaccg ggtgcgaggt ggcaaaggtg agcgccgtgg tcacgccgtg
    14581 gcgcagcagc tcggcaaaga acacgttcgc catctcggcc gcataggccg gcgaatcgaa
    14641 gcgggcctcg gccgggaagg tgtagttctc cagccagggc agcaggccgt cggccggcga
    14701 gccgatgatg tcggtctgcg ggaaatgcac gtgcatgtcg atgaagcccg gcgcgatcag
    14761 gcgaccgggc cagtgtgtca ccggcacctg cgggtggcgc gccgccaccg accggtaggg
    14821 gccggcgtcg atgacgcgct gcacgccatc gacgcccagg cccaccacca gcagcccgtc
    14881 gctgtcgtgc agcggggcgc cgtgggggtc gaaacgcagc agctctgccc ggtacgcgtt
    14941 cacggcgact caacctcccg gaatgtagaa gaacttgaac aggaacacgg cagcgatcag
    15001 ccagaccatc cagtgcactt cgcgcacccg gccggtcagc agcttgatgg cggcgtaggt
    15061 gatgaagccg aaggccaggc cgttggcgat ggagtaggtg aagggcatca tcagcgccgt
    15121 caccgccgcc gggatcactt cggtggaatc ttcccattcc agctcggtca ggtcacgcat
    15181 catcaggcag ccgacgaaaa acagcgccgg cgccgtggcg taggctggca ccgcaccggc
    15241 cagcggtgcg atgaacaggg cggccaggaa cagcaccgac accgtcaggg ccgtcaggcc
    15301 ggtgcgcccg ccggcctgca cgcccgaggc gctttcgacg taggcggtgg tgctggacgt
    15361 gcccagcagc gagccggcaa agatggcggc gctgtcggcg aacagcgatt tgttgagccg
    15421 gtccatgcgg cccggcacca gcaggccggc acgccgcgcc acgcccatca gggtgccggt
    15481 ggcgtcgaac agttcgacca ggaagaacac cagcaccacg ttcagcagac ccttggtcag
    15541 cgcggtcatg atgtcgagct tcagaaatgt cggctcgatc gatggcgggg ccgagaagat
    15601 gccgcgaaat tcgttgccgg cgaagaagaa cgaggcgatg gtgacggcca cgatgccgat
    15661 caggatggcg ccgcgcaccc gcagcttgtc cagcgccacc atcagcagaa agcccagcac
    15721 ggccaggatc acgctgggct ggtgcagatc gcccagggtc accagggtgg ccttgctgcc
    15781 caccacgatg cccgcgctct tgagcgcgat cagcgccagg aacaggccga tgcccaccgt
    15841 gatggcgtag cgcagcgact gcggaatgcc ggcgatgatc aggccgcgga tgccggtcag
    15901 ggtgacgaac aggaacaggc agcccgaaat gaacaccgcc cccagcgccg cctgccaggt
    15961 gaagcccatg tgcagcacca cggcgtaggc gaagtaggcg ttcagcccca tgccgggcgc
    16021 cagcgcgatc gggtagttgg cgtagaaggc catcaccgcc gtgcccagcg cggcgatcag
    16081 acaggtggcg acgaacaccg cgtccttggg catgccggcg tcgcccagga tcgccgggtt
    16141 gacgaagatg atgtaggcca tcgtcaggaa ggtggtcaac ccggcgatca gctcggtgcg
    16201 tacgttggtg ccgtgttctt ccagcttgaa caggcgctcg atccaactcg tgctcattgt
    16261 ggtggtctcc tcgtcatggc ttgtgggaag gggtggacgg gtggcgtggc cccccggtgc
    16321 gcagttcgct gatcacctgt cgtgtgcgtt cgcgcagcca gcgcgcagcg ttggcgtgct
    16381 gactgcgggt gtgccacagc atgtagtaca gcagcggcgg catggcgacc ggacagggca
    16441 ggatggtgag cggcagctgc ctggcgtagc gctcgcagta ctgccggccc gtggtcagca
    16501 ccagcaggct gtcggccacc atttgcggta tcaggccgaa ataggcgcag cgcacggcga
    16561 tgcgccgctg caggccgcgc tcgtcgagca cctggtcgat gatgccgcgt gctcccgggt
    16621 agttgggcat gggggcgatg tgctcggcct ccagccagtc ggcttcgctc cagccgcggc
    16681 gcgcggccgg gtggcgatgg ctgaccagcg acaccacttc gtcgctgaac agatgcgcca
    16741 tgtgcaactc gtcgggcggt tgcagccagt tgccgatcac caggtcgatc tcgccctgcg
    16801 ccagctgcgc gcggtagtcg gcctcgcccg tcagcgcatg gatctccacc cgcgtcagtg
    16861 gcgcctggcg ctggatgtcg gccaccagat ggggcagaaa cagcgggtcc aggtagtcgc
    16921 tggcggcgat gtgaaagcgc tgcgtgctgg tgcgcggatc aaagcgctgg gcctcggtga
    16981 acagcgtctc ggccgtgcgc aggatgctgg cggcgggctc gatcatgcgg cgtccggcat
    17041 cggtggccac catctgcgcg ccatggcgca ccagcagcgg gtcgcccgcc agatcgcgca
    17101 ggcgcttgag cgccgccgac accgccggct ggtgcatgcc caggcgcagc gcggcgcgcg
    17161 agacgctgcc ttcggtcagc acggtgtgca gcacgcggat caggtgcagg tcgatgcgct
    17221 ccagcggccc gcgatcgccc gtcgagccgg cgggcgcagg gttgggcgcg acatccttgg
    17281 tgttcatgaa cgggcaactc cggattcgat gcggcggcaa gacatggcgc cagcggctca
    17341 ggcgcgcacc gcctgcagcg cgcgcaggat ggcttcgctg gtggcgggcg cctgcagcgg
    17401 cgggtcgatg cggtgaccgc ccgccgccga aacggcgtcg cgaatcgcaa agaacaccga
    17461 aaacggcagc agcagcggcg gctcgcccac ggccttgctg cggtggatgg cgtcttcggc
    17521 gttgcggccc tcgaacaggc gcacgttgaa caccggcggg cagtcgttgg ccgtgggaat
    17581 tttgtaggtg ctgggcgcgt gggtgctcaa gaggccggtc ttggggtgcc agaccagctc
    17641 ttccatggtc agccagccca tgccctggat gaaggcacct tcgacctgcc cgatgtccag
    17701 cgccgggttg agcgagcggc cggcgtcgtg cagcacgtcg gcgcgcagca gcttccactc
    17761 gccggtcagg gtgtcgacca gcacctcgct caccgccgcg ccgtaggcaa agtagtagaa
    17821 cgggtggccc tgcatcttct cgcggtccca cgacagaccg ggcgtggcgt agaagccatc
    17881 cgaccagagc tgcacgcggt ccaggtaggc ctcgccgacc acggtggcaa agtccagcgt
    17941 ccggccaccc acctgcacct ggtcgttggc aaagcgcacc tcggcggcca acccgccgtg
    18001 gcgcccggcg gcgcaggcgg ccagccgttc gcggatctgg cgcgccgcgt cctgcgccgc
    18061 cttgccattc aggtcggagc cggtggacgc cgccgtggcc gaggtgttgg ccaccttgct
    18121 ggtgtcggtg gcggtcacgc gcacgcgctc gaagcccacg cccagctcat gcgccaccac
    18181 ctgcgccacc ttggtgttca gaccctggcc catttcggtg ccgccgtggt tgaccaggat
    18241 cgagccgtcg gtgtagacat gcaccagcgc gccggcctgg ttgaagtgcc tgacgttgaa
    18301 gctgatgccg aacttcagcg gcgtcagcgc gatgccgcgc ttgagcaccg ggctgccggc
    18361 gttgaaggcc gcgatggcgg cgcgccggcc gtcgtagtcg ctgctggcct ccagctgatc
    18421 gaccagctcg tggatcacgt tgtcgcgcac ggtctggccg tagggcgtga cgttgcgttc
    18481 gctctgcccg tagaagttgg cccggcgcac cgccagcggg tcgcggccca gccggcgtgc
    18541 gatgctgtcg aggatgtatt cgatggcgat cgccccctgt gggccgccaa agccgcgaaa
    18601 cgccgtgttg ctctgcgtgt gggttttgcc acagtagccg tgcatggcca cttcgggcag
    18661 ccagtaggcg ttgtcgaagt ggcagatggc gcgcgtcatc accggcgccg acaggtcggc
    18721 cgaatggccg gcgtgcgaca ccatctgcag gcaggcgccc aggatgcggc cctcatcgtc
    18781 gtggccgacc tcgtactcgt accagaagcc gtggcgccgg ccggtgacca tgaagtcgtc
    18841 gtcgcggtcc agccgcagct tgaccgggcg ctgcaacgcc tgcgccgcca ccgccgcgat
    18901 gcaggcaaac tgcgccgact gcgattcctt gccgccaaag ccgccgccca tgcgccggca
    18961 ttccaccagc acggcgtgcg cgggcacgcc cagcgcatgc gccaccaggt gctgcatctc
    19021 gctcgggtgc tgggtcgagc agtgcacgtg catgccgcgc ccttccttgg ggatggcgta
    19081 gctgatctgg ccttccaggt agaactgctc ctgcccgccc acgtccaggc tgccgctcag
    19141 gcggtgcggg gcgcgggcga tggcggcccg gatgccggcc tcgtccagcc cgctggcgct
    19201 gcgcaccagg tgcatgggtg gcaccacgta ctgacccttt tcgtgcgcct ggcgcggggt
    19261 cagcaccggc tcggcggcgt ccacccgcag cacctgcctg gccagcgccg cggcgcggcg
    19321 tgcctgctcg cgcgtttcgg ccaccacggc aaacaccggc tggcccagga agcggatctc
    19381 gccgtcgcac aggatcggct cgtcgtgcac gatcgagccg cagtcgttga cgcccgggat
    19441 gtcggccgcc gtgagcacgc gcaccacgcc cggcatggcg gccagcgcgt cgcgatcgaa
    19501 gccctgcagg cggccggcgg ccacgggcga caggccgagc gcgcagtgca gggtgccggc
    19561 cagctcgggc aggtcgtcaa tgtaggtcgc gccgccggtc acgtgcaggg tggccgattc
    19621 gtgcggccgg ctgatgccca cgcgcgcgcc ggcttgctcg gcctgggctt cggcggcgct
    19681 gtccaggcgg gcctgctggt tgtggcggta gtcggcaaag ggcagggcgg cgtgcagcag
    19741 ggggtcggca acagggtggt tcatggtcgg gctcccggta gccgctcagg ccgcggcgtg
    19801 cggcatgagg tggaacacgc tggtggcggc cggtggcagc gggttttcgg gccgggtttc
    19861 cagccacagg cgctgcagca ggttctgcgc cacctgcagg cgataggcgc tgctggcgcg
    19921 catgtcggtc agcggcgtga agtcgcgcgc cagcgcctgc tgggcggtgc gcacggtggc
    19981 ttcgctccag ggctgcccgg tgatggccgc ctcggcctcg gcggcgcgcc ggacggtggc
    20041 ggccatgccg ccgtaggcca ggcgcacgct gcgcaccacg ccggcgtcca gttcgatcgc
    20101 catgccggcg cacagcgccg agatgtcgca gtcgaagcgc ttgctgacct tgtagccgcg
    20161 cagctggcgc cggaaggcgg cgtcgggcac gacgatggcc tgcacgaact cgccgtcttc
    20221 gatggcgttc ttcatgtagt ccaggtaaag ctcggtgagc ggcaggcggc gcacgcgctc
    20281 gccccggcgc agctcgaccg cggcgtcgag cgccatcagg atcggcggcg catcgccgat
    20341 cggcgagccg ttggccacgt tgccgcccag ggtgccagcg tggcggatcg gcagcgaggc
    20401 aaagcgcagc cagacgtcgg tcagcgtcgg ggcgcggcgc accagggcgc gccaggcgtc
    20461 ttccagtgcg gcggcggcgc cgatccacag tgcgtcgccg cgcgcttcga tcgcgcgcag
    20521 ctcggccacg tggccgacgt agatcaggtc gcccacgtcg cgaaactgct tgttgaccca
    20581 caggccgatg tcggtcgagc cggccagcag gcgggcctgc ggtttggcca ggcgcagctc
    20641 ggccagctgc tgcaacgtgc gcggggcgtg gaagtggtcg gtgcgcgggc ccgcgggcgt
    20701 gggctgcggc gccgcgtaat ggagcgccgg ctcgcgctgc agggcacgca gcgcctcggt
    20761 cacggcctga cggtcaagcg ccaccggcgg caggtcgaac atgcgttggc cggcgtcgag
    20821 gatgggacgg tagccggtgc agcggcacag gttgcccgac aggtcgtcgg ccagctgctg
    20881 gcgcgtgggc tgggtgccgg cctgctggtg gcgttcatag gtgttccaca gcgacatcac
    20941 gatgcccggc gtgcagaagc cgcattgcga gccgtggcac tccaccatgg cctgctgcac
    21001 cgggtgcagc gcgtcgtgcg cggcgccgcc cagcgtctgc aggtcttcca ccgtgaacag
    21061 cgccttgccg tccagcgtgg gcaggaactg gatgcaggcg ttcacgctgc tcaggc
//
LOCUS       DAOQTZ010000045        20892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_2981_length_20892_cov_13.265297, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000045 DAOQTZ010000000
VERSION     DAOQTZ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 20892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20892
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2981_length_20892_cov_13.265297"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..2162)
                     /locus_tag="PKN51_07115"
     CDS             complement(51..2162)
                     /locus_tag="PKN51_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84906.1"
                     /translation="MPAPRTFLGWGVGMTFALLAAVVLALLRLPVTGLQLAPADEKLL
                     VVARHQHAPAVPVGSRLQLDDAPLAAEDLLAHQHDSFADWRRADAARAALDRLHHSAA
                     AGWPAQVDGLPSRLHTRARQLEELPVGLWLHLGVGAMFMLVPLTVLSLRPRDAVARHF
                     ALLGLGILVSITSDAVFRYRELAFPAAWLAWLGPLNHAGNLLAGAALLALMACYPTPL
                     HRAPMLVRLSYTSAALLWLGLQVRVFDGPDRGVPMVLGYLLASIVPGVLQWRRTARRP
                     VERAALRWFVLTFGLSLAMVALLLLVPVLLGLSAPPPLLVNLGLIALLVVPLGMALGI
                     TRYRLFELDRWWNSVWTWFFGGVAVMLLDAALIAVLGLSQWQAMPLALALLGWLYFPL
                     RQWVWQRWFGGSTPSSEWPRALYAATDGATLTQQWIDALRDRFRPLDIALLPAPVPHA
                     RLGEHGVQLDLPGLEPDAPGLRLRHAHSGARLFSGADIQAATAMLSIARALHAALHER
                     DRAVASERSRIRRDLHDDLGAKLLTLLYQVDAPQQSLVRSAIRDTQDILTTLDAHALP
                     LTEAVALWRDEAGLRADTHGFMLHWQSDALDSTALLSARQHTDLARILREAITNAVRH
                     AQARVIAVTLRTPGDGWLHLSVRDQAHQPSATQPSDGSGAGTRQIARRASDLGGRACW
                     HTMPDGTQLDVCVPLTTPVQG"
     gene            complement(2352..2993)
                     /locus_tag="PKN51_07120"
     CDS             complement(2352..2993)
                     /locus_tag="PKN51_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT84907.1"
                     /translation="MKSCLIIEDMDATARVTAARVAAAFPGIDVGLASTLAAARGWLR
                     SNPPPALVLLDVGLPDGEGTELLLDGALGAGSLVIVHTVFGDDEHLFQALRAGAHGYL
                     LKEDSEADFLTALSGIANGRPPLSAPMAQRMMVFFRADRDTPGVALSPREREVLTLIA
                     RGLTVRQAAQALALTENTIASYLKTVYQKLHVGTRAAAALEAIRLGLVRPPGR"
     gene            complement(3068..3604)
                     /gene="ruvC"
                     /locus_tag="PKN51_07125"
     CDS             complement(3068..3604)
                     /gene="ruvC"
                     /locus_tag="PKN51_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNT84908.1"
                     /translation="MRILGIDPGLRTTGFGVLDAEGPRLCYVASGVIQTGSAAQGNLP
                     ARLKLLFDGITELQARYTPDVAAVEIVFVNVNPQATLLLGQARGACLTALVAADLPVA
                     EYTALQMKQAVAGHGKAAKTQVQAMVQRLLGLPAAPRPDAADALGLAITHAHAGPAVA
                     RLHAAGAERTTSSMYRRR"
     gene            complement(3752..4957)
                     /gene="ppk2"
                     /locus_tag="PKN51_07130"
     CDS             complement(3752..4957)
                     /gene="ppk2"
                     /locus_tag="PKN51_07130"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNT84909.1"
                     /translation="MSSANHQPQGSASSRKNVAPKTGKTSKTAASSRRANGDLRAVRL
                     GDTPPSLTARAIEDHVVAHATAGARDAQAQAALRLVNDPAAGTPSERASALRALLDGA
                     SPDDRKALRKVLKANGRDTLATKSNPDEELAADWRDGGYPYEHLLRRATYEQQKFKLQ
                     VELLKLQNWVKETGQRVVIVFEGRDAAGKGGTIKRFMEHLNPRGARVVALEKPSEIER
                     GQWYFQRYVQHLPTHGEIVLFDRSWYNRAGVERVMGFCSEREYQDFMHQAPEFERHLV
                     SSGIHLIKFWFSVSRAEQRRRFKERENHPLKQWKLSPIDKASLDKWDDYTRAKEAMFF
                     ETDTADSPWTVIKSDCKKRARLNAMRYVLHKLPYTGKDLAQIGKLDPLIVGRAHVVYE
                     RGERPQALM"
     gene            complement(5112..5702)
                     /locus_tag="PKN51_07135"
     CDS             complement(5112..5702)
                     /locus_tag="PKN51_07135"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNT84910.1"
                     /translation="MSKFDKKALRERLVRERLHLEDRLARAAALQDIMRIWLLGRPDT
                     VVGAYWPIKGEFDPLPALHRWKEDGELMDEPQRRRIGLPVIDKVHKTLTFHVWYPGCP
                     MEEDAYGIPKPKDTELIVPTLLFVPCVGYGPGGYRLGYGGGFYDRTLAALQPRPYTAG
                     LGFTQGFVDDFEPEPHDQPLDAILNDNGVVWPVMRA"
     gene            5877..7817
                     /locus_tag="PKN51_07140"
     CDS             5877..7817
                     /locus_tag="PKN51_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNT84911.1"
                     /translation="MLALPADAAARDAVLLDMRDAHRRGDKARLTALLPQARGHALEP
                     WAAYWELKARLEDAAASEVQDFLARYAGSYQEDRLRNDWLLLLGKRRDWAAFRAEYPR
                     YRMRDDRELRCHAVSADLAAGLAPGPQAVEQVRRDWLAQRDADDGCLYAAGLLHQTGL
                     LPYQVLWKKARLAMEANRPALARAAVALDAPDAAAELASIAQSAPRYLGGRASSATHK
                     RQELATLALIRLATQAPDQAAQQLEDKWAALLDREERNWLWGVIGKWSALAPSERAHG
                     YFSRVTREGDLSDELLAWKVRAALRQGDWRAVLRSVDAMQDAAADDGAWLYWRARAQQ
                     ALARHDDERAAARAGFERLTAQVGRQGFYEKLALEALQRPIVAPPAPAPLTEAEREAA
                     RRHPGLGRALLAIALGLRGEGVREWNYWTNLHRPGGMGERELYAAADLACARQVWDRC
                     INTSERTRSFADFGQRFPTPHREAVQRQAQAIGLDAAYVYGLIRQESRFVTDARSGVG
                     ASGLMQVMPATARWTARRIGLSGFVPAQINDLDTNILIGTSYLKLALDDFQGSLPLAA
                     AAYNAGPGRPRAWRNGPLLDGAVWAENIPFNETRDYVKKVLSNTVDYAGLLTGQPQSL
                     AARLGRVGPLAPGQADPSMGLP"
     gene            7894..8862
                     /locus_tag="PKN51_07145"
     CDS             7894..8862
                     /locus_tag="PKN51_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HNT84912.1"
                     /translation="MGKRILVLGGSGFVGRHLIEHLQRLGVRVTVPTRRRDAAPWLWS
                     LPRVDVVPADVTDPAHLGALLAGHQAVVNLIAVLHGDARRFEQVHVELPRRIAAACAA
                     SGVRRLVHISALGAAADGPSRYQRSKAQGEAVLQQAAVDGALALTLLRPSVIYGSGDH
                     LLNLFAGLQRLFPLIPLASAEARFQPVWVRDVAQALVRCLQDDTTAGRTYELCGPEVW
                     TLRELVRAAGMWAGVRGGRGRPIVGLPAALGRLQALAMECLPGEPLMSRDNLDSMRVD
                     NVASGQLPGLAELGITPAPLSAVGPMIVGADHPAARLDAYRRSAGR"
     gene            8884..9579
                     /locus_tag="PKN51_07150"
     CDS             8884..9579
                     /locus_tag="PKN51_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNT84913.1"
                     /translation="MLKLYIGNKNYSSWSMRPWVLMKQAGIAFEEVQLRFDSFSGDSQ
                     FKQRLGRISPAGKVPVLTDGELVVWDTLAIAEYLAEKFPELHLWPRSAPERARARSVC
                     AEMHSGFGALRSACGMNIEAQLPEAGAIIWRDRPDVRADVARIVEMWQELLAMHGGPM
                     LFGRFGIADAFYAPVVMRLKTYALPVPDDVAAYMARLCALPGVAAWIEGALAEQDFLP
                     FEEPYRAARPASA"
     gene            9726..10913
                     /locus_tag="PKN51_07155"
     CDS             9726..10913
                     /locus_tag="PKN51_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNT84914.1"
                     /translation="MSIDDRAQPPAALPGWPLLRLGFRPFYLGTALLACVSIPLWIAA
                     FLGHMPLRLPFPILLWHAHEMLFGFAAGVVIGFLLTAVRAWTGLETPRGPVLGLLALL
                     WLAARLAAVLAPYPLFAVLDLLLLPAVAFFLLRVLIRAGNRRNIPLISLLLAMALANA
                     VFHLSVLGLIAVPAMSALYAELALILMVVSIMTGRVVPMFTKNVTPGLVIQVSRRFEL
                     TLLAVTAVTLSLWVLGAPAWLVLPASALSAALHALRLWKWHPRVTLRRPILWILHFSY
                     AWMPVGFALLALAQPGWVAPSLAVHAFGVGVIGGLIIGMITRTARGHTGRPLQASRGE
                     VVAYALVMLATVLRVLVPAVVPAWYVRALDSAAGLWALAFAIYLVIYVPWLTRTRLDG
                     KDG"
     gene            11000..11290
                     /locus_tag="PKN51_07160"
     CDS             11000..11290
                     /locus_tag="PKN51_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNT84915.1"
                     /translation="MSTATLAEIDVRSIPPYQRHQQIFAQVDALQVGQALQIVNDHDP
                     VPLRRQLESRSPGQLQWAYVQAGPDLWRVQISKVQGAADAEGSCCSGGACCG"
     gene            complement(11357..11911)
                     /gene="sufT"
                     /locus_tag="PKN51_07165"
     CDS             complement(11357..11911)
                     /gene="sufT"
                     /locus_tag="PKN51_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HNT84916.1"
                     /translation="MMNRRHREDVLVRRHVTVETIPSGHPVELEPGQLAQITQALGGS
                     FTLLVEGQLMRLKGQDADAIGKQPPEALAVPKDLPIEELEPLVWDTLRTCYDPEIPVN
                     IADLGLVYRLAFEPLDDPDQVRVVIDMTLTAPGCGMGESIANEVCDKVLALPRVGEIT
                     VNLVFDPPWDRSMMSEAAQLALGL"
     gene            complement(11908..12369)
                     /locus_tag="PKN51_07170"
     CDS             complement(11908..12369)
                     /locus_tag="PKN51_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNT84917.1"
                     /translation="MSSAENDLYQEVVLEHKRAPRNFGHLPQPTHQAQGTNPSCGDRV
                     AVELLLDGERVQDIRFSGQGCAICMASSSMMTEAVKGRDIAAAQALQQHFRAVLTGEE
                     DPDEAPLGKLVSLAGVRQYPSRIKCALLGWHALMHAIAGQQDAGGTEESLP"
     gene            complement(12366..13655)
                     /locus_tag="PKN51_07175"
     CDS             complement(12366..13655)
                     /locus_tag="PKN51_07175"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HNT84918.1"
                     /translation="MPNHQPPLTLPEADRAAAAPPPLAAWRSQFPVLSQQIHGHPLAY
                     LDNAATTHMPLAVMEAMRRFETHERANIHRGVHTLSQRATDAFEQARATLKRFIGADA
                     GHELVFTSGTTESLNLVAQGLWGAGPGQAWLRPGDRIISTGLEHHANLVPWQHAARMS
                     GAELVVLRPDAQGRLQQDALAALLTPRTRVLAITASANATGERPPYEAMLAMARQAGA
                     LTVLDAAQAVGHGMPALSALACDFMAFSGHKMYGPMGIGVLAGRRDALERLHPLRLGG
                     DMVEDVSYERAIYAALPARLEGGTPNVAGAVGLAAAATFIDRIGRAAIDGHLHALRAQ
                     AVAGLSALPGVTVLAPHATQAALVSFVADQVHPHDIGTLLDERGIAVRTGHHCAQPLH
                     DHFGLGPTTRASFALYNSAEEVERLIAAVARALEMLR"
     gene            complement(13713..14945)
                     /locus_tag="PKN51_07180"
     CDS             complement(13713..14945)
                     /locus_tag="PKN51_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNT84919.1"
                     /translation="MNPSQAERLAARQHLQRQGWIARRSEAFHHLPPPELAHWLGQPD
                     ADADDGNAPALAGAGWTLHPLGHGGAHGVDARWLDALDPAQRAELLAGLPPPGDGEAA
                     PFVWAHRALCRQGLRLRLSATPGQPAAPVVLQLRHQPHQRAEAPLLVLEVDAGVHCVL
                     LETHERTAADGAHPVTQNLHGHIRLAAGAHLQHLRVVAPQAQDQIAHHLHVSLAPAAH
                     YAQALVATGSAYHLQRSTVQLSGRGARAQQAALLLLDDQQLDHQAHSDLAAAHSASAV
                     ESLVLAQGGARAVSNAYTRIAAGADEAEVRQRLSGIPLGGQPRLILRPHLEILHDNVQ
                     AAHGATWGALPADALFYARQRGLDEASARALITEGMARAVLERSLGDTELLSDWLATG
                     WLTQRMARQARQRAETPA"
     gene            complement(14947..15717)
                     /gene="sufC"
                     /locus_tag="PKN51_07185"
     CDS             complement(14947..15717)
                     /gene="sufC"
                     /locus_tag="PKN51_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNT84920.1"
                     /translation="MTSPEPLLQVRDLRVQVGERTVLHSVSLDVNAGSLVVLMGANGS
                     GKSTLGLALAGHPAYQVLGGQALLAGKDLLALPAHERARAGLFLSFQSPPDVPGVKNN
                     LFIRTALNAQRESRGQPPLDAFDFLTSAKQAAKALGLPEAMLGRPVNEGFSGGERKRN
                     ELLQLALLQPRLALLDEIDSGMDVDGVRAVVEQVRRQRAQGTAFIVVSHYLQMIEQLE
                     PDAVLRLDQGCIAETGDLGLARHIAETGFASRTAPAGA"
     gene            complement(15799..17289)
                     /gene="sufB"
                     /locus_tag="PKN51_07190"
     CDS             complement(15799..17289)
                     /gene="sufB"
                     /locus_tag="PKN51_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNT84921.1"
                     /translation="MASPEPISTAQALADKPQEAPLEQALQRPYQHGFVTDIESDSLP
                     PGLDEGTIRAISRKKKEPQFLLDWRLAAFRRWQTMALPDWARLKIAPIDFQALSYYSA
                     PKSMKDKPKSLDEVDPKLLETYEKLGVPLHERAALAGVAVDAVFDSVSVGTTFRKQLA
                     DVGVIFCSFSHAVQNHPELVEKYLGTVVPQGDNFYAALNSAVFSDGSFVYIPKGVKCP
                     MELSSYFRINAQNTGQFERTLIIAEEGSSVSYLEGCTAPQRDENQLHAAVVELIAHDD
                     AFIKYSTVQNWYPGDEHGVGGIYNFVTKRGLAAGKRSRIAWTQIETGSAITWKYPSCV
                     LRGEGSSGEFFSIAVSNHRQQADTGTKMIHLGKNTTSRIVSKGISAGHAHNAYRGLVR
                     INPGAEGARNHTQCDSLLIGPHCGAHTFPYVDSGRADAVVEHEATTTRISAERLFYCE
                     QRGIDAQEATALIVGGFCQAVLEELPMEFALEAKALLAVSLEGAVG"
     gene            17435..18712
                     /locus_tag="PKN51_07195"
     CDS             17435..18712
                     /locus_tag="PKN51_07195"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNT84922.1"
                     /translation="MQIYMVGGAVRDRLLGLPVQDHDWVVVGATPEDMVARGFVPVGR
                     DFPVFLHPSSHEEYALARTERKSGRGYKGFTVFTSPEVRIEDDLARRDLTINAIAVRA
                     GQASAGATFDHEDGVIDPFGGRRDLQARVLRHVTDAFREDPVRILRVARFAARFHDFS
                     VAPETRALMRQMVAEGEADHLVPERVWQELARGLMESQPARMFEVLRDCGALAVVLPE
                     LDRLWGVPQTASHHPEIDTGVHVMLVLQMAARLQAPLAVRWACLMHDLGKGETPADLL
                     PRHLGHEQRSARLLRAVGQRLRVPTDCTELAEVVAREHGHIHRSAELGAAALLRLLQR
                     CDALRKPERFAQVLLACECDARGRLGFEHSAYPQRARLQAALGAAQSVATAGLAQAAQ
                     AAGARGADIGQAVAQARTRALQAWLDGSAPAAV"
     gene            18838..19461
                     /locus_tag="PKN51_07200"
     CDS             18838..19461
                     /locus_tag="PKN51_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT84923.1"
                     /translation="MARPISAAHGLRRDEILDVAADCFARQSYPATSMNDIAAACGTS
                     KARLYHYYDSKDAILFELLDRYTQRLLVIIGQVQATAHRQALDERATLHALIRAFLRE
                     YESSATRHAALLASTQFLGETQRGVILDRQRDVVSAVTRFLRRAYPERVDARNQTAIT
                     MTLFGMINWTFTWLRPGGSLSYDAFADEVIALLERGLSSPLNPHSRS"
     gene            19458..20891
                     /gene="glmU"
                     /locus_tag="PKN51_07205"
     CDS             19458..20891
                     /gene="glmU"
                     /locus_tag="PKN51_07205"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNT84924.1"
                     /translation="MNPIDIAVMAAGKGTRMKSRTPKVLQKLAGRPLLGHVLDTAAQL
                     RARSVVVITGHGATEVEAFGASLAGAGAGFDLQFARQEPQLGTGHAMQQAMPRLADDG
                     LVVVLSGDVPLTEAATLQALVDAAQGERLALLSVTLADPSGYGRIVRGAGGAVQRIVE
                     HKDASAAERAIGEIYSGIMAVPARLLRGWLTRLTNHNAQGEYYLTDVVAMAVADGVPV
                     VAHCIQDALQVAGVNSPLQLAELERAHQLRQARALMEAGVRLADPARFDLRDDARRGV
                     RAELRCGPDVDIDVGCIFTGRVEIGEGAQIGAHCCIANARIEAGAVIHPYTHIDGEAA
                     GVHVGPGALVGPFARLRPGADLGPEVHIGNFVEVKNSSLAAGAKANHLAYLGDAVVGE
                     RVNYGAGSITANYDGARKHRTVIEADVHVGSNCVLVAPVTIGAGGTVGGGSTITRDTP
                     PGGLSVARGRQAHIANWQRPAKPQGGG"
BASE COUNT         2873 a         7176 c         7658 g         3185 t
ORIGIN      
        1 gccggcgaac ggcccgcaaa ccaagtctag gttggcgtca gcgcgcggtg ttagccctga
       61 accggggtag tcagcgggac gcagacgtcc agttgggtgc catccggcat ggtgtgccag
      121 caagcgcggc cgcccaagtc gctggctcgg cgggcaatct ggcgcgtgcc ggcgccactg
      181 ccgtcagacg gctgggtggc cgacggttgg tgcgcctggt cgcgcacgct caggtgcaac
      241 catccgtcgc cgggcgtgcg caaagtcact gcgatgacgc gggcctgggc gtggcgcacg
      301 gcgttggtga tggcttcgcg caggatgcgc gccaggtccg tgtgctggcg cgcgctcagc
      361 agggcggtgg agtccagcgc gtcggactgc cagtgcagca tgaagccgtg cgtgtcggcg
      421 cgcaggcccg cttcatcgcg ccagagtgcc accgcctcgg tcagcggcag ggcgtgcgcg
      481 tcgagcgtgg tcagaatgtc ctgtgtgtcg cggatcgccg agcgcaccag ggactgctgc
      541 ggcgcgtcca cctgatagag caaggtcagc agtttggcgc ccaggtcgtc gtgcagatca
      601 cgccggatgc ggctgcgttc ggaggccaca gcccgatcgc gttcgtgcaa cgctgcgtgc
      661 agcgcgcggg cgatggacag catcgccgtg gcagcctgga tgtcggcgcc gctgaacagt
      721 cgcgcgccgc tgtgcgcgtg gcgcagccgc aaccccggcg catcgggctc caagcccggc
      781 agatcgagct gcacgccatg ctcgcccagg cgcgcatgcg gcacgggcgc gggcagcagc
      841 gcgatgtcga gaggccgaaa gcggtcacgc agcgcgtcga tccactgctg tgtgagcgtc
      901 gcgccgtcgg tggccgcata cagggcgcgc ggccactccg aggagggtgt cgatcccccg
      961 aaccagcgct gccagaccca ctgtcgcaat gggaagtaca gccaacccag caacgccagc
     1021 gccagcggca tggcctgcca ctgcgacagg cccagaacgg cgatcaacgc ggcgtccaac
     1081 agcatcacgg cgacgccgcc gaagaaccag gtccagaccg agttccacca gcgatccagc
     1141 tcgaacagcc ggtagcgcgt gatgccgagt gccatgccca gcggcacgac cagcaaggcg
     1201 atcaggccca ggttgaccag caacggtggc ggcgcgctca agcccagcag caccggcacc
     1261 agcaacaaca gggcgaccat ggccaggctc aggccgaagg tcagcacgaa ccatcgcagg
     1321 gccgcgcgct ctacgggccg gcgtgccgtg cgacgccatt gcaaaacacc gggcacgatg
     1381 ctcgcaagca gatagcccag caccatgggc acaccccggt caggtccgtc gaacacgcgc
     1441 acctgcagac ccagccacag cagagccgcg ctggtgtagg acaggcgcac cagcatcggg
     1501 gcgcggtgca gtggtgtcgg gtaacacgcc atcagcgcca gcagggcggc gccggccagc
     1561 aggttgccgg cgtggttgag cggccccaac cacgccagcc acgcggcggg gaaagccagt
     1621 tcgcggtagc gaaacacggc gtcgctggtg atcgacacca gaatgcccag gccaagcagc
     1681 gcgaagtgcc gtgccactgc atcgcgtgga cgcaacgaca gcaccgtcag cggcaccagc
     1741 atgaacatcg ccccgacacc gaggtgaagc cacagcccga caggcaactc ctcaagttgg
     1801 cgcgcacgag tgtgcagccg tgacggcagc ccatcgacct gcgcaggcca gccagcggcc
     1861 gcactgtggt gcaagcgatc cagcgcggcg cgcgcggcgt cggcgcgtcg ccagtccgca
     1921 aagctgtcgt gctggtgagc gagcaagtcc tcggctgcca gtggcgcgtc gtccagttgc
     1981 agccggctgc cgacaggcac ggccggcgcg tgctggtgtc gggcaaccac gagcagtttc
     2041 tcgtccgccg gggccagttg cagtccggtg accggcagcc gcagcaacgc cagcaccacc
     2101 gcagccagca gcgcaaacgt catgccgacg ccccatccca gaaaggtacg cggcgctggc
     2161 atggtgatga acgtgtcccc gcggcggtgc gctcagaagc cgggcatgaa tgccgagtgc
     2221 ccgagcggcg tgtcccctcg cggccgaaca aggcgccaga cgcaacccgg gctggagcga
     2281 tcatgggcaa cgccgagcgg gagtgtcggg gcggcggtca aggtgggcat gaggggaccg
     2341 ttcctgcctt ctcagcgtcc cggaggtcgc accaggccga gccggatggc ctccagcgcg
     2401 gcggcggctc gggtgccgac gtgcagtttc tggtacacgg tcttcaggta actggcgatc
     2461 gtgttttcgg tcaacgccag ggcctgcgcg gcctggcgca cggtgaggcc acgggcgatc
     2521 agggtcagca cctcgcgttc acgcggtgac agcgccacgc ccggggtgtc gcgatcggcc
     2581 cgaaagaaga ccatcatgcg ctgcgccatg ggggcggaca gcggcggccg accattggcg
     2641 atgccactga gcgccgtgag gaagtcggct tcgctgtctt ctttcagcag atagccgtgg
     2701 gcgcctgcgc gcagggcctg gaaaaggtgc tcgtcgtcgc cgaacacagt gtgtacgatg
     2761 accagcgaac cggcgcccaa tgcgccgtcc aacagcagct cggtaccctc gccatcgggc
     2821 aggccgacat cgagcagcac cagggctggc ggagggttcg aacgcaacca gcccctcgct
     2881 gccgccagcg tgctggctag gccgacgtcg atacccggaa acgccgctgc aacgcgcgca
     2941 gccgtgactc gggccgtggc gtccatgtct tcgatgatca gacagctttt catgcgtggc
     3001 gtgcgtctgg tgcctcgatg ggtgggggtg acgaaggtag cacagacctt cgacggtgcc
     3061 gtgcgggtca acggcgccgg tacatgctgc tggtggtgcg ctcggcgccc gccgcgtgca
     3121 ggcgggcgac ggcagggccg gcgtgcgcgt gggtgatggc caggcccagg gcgtcggcgg
     3181 cgtcggggcg cggtgcggcg ggcaggccca gcagacgctg caccatggcc tgcacctggg
     3241 tcttggcggc cttgccgtgg ccggcgacgg cctgtttcat ctgcagcgcg gtgtattcgg
     3301 ccaccggcag atcggcggcc accagcgcgg tcaggcaggc gccgcgcgcc tggcccagca
     3361 gcagcgtggc ctgggggttg acgttgacga acacgatctc gaccgccgcc acgtcgggcg
     3421 tgtagcgcgc ctgcagctcg gtgatgccgt cgaacagcag cttgagccgc gccggcaggt
     3481 tgccctgtgc ggcgctgccg gtctggatca cgccgctggc gacgtagcac aggcgcgggc
     3541 cctcggcgtc gagcacgccg aagccggtgg tgcgcaggcc ggggtcgatg ccgaggattc
     3601 gcatggattg ggtgtcgcgc cccggagtgg atgcagccgg ttgtcgatca gcagggcgct
     3661 ttcataatga tagctgctcg cgcgctaccc ctgggcgttg caatgcaata tatccaggat
     3721 cgggcgcgac ccggccggcg cccgtcggcc ttcacatcag cgcctggggg cgctcgccgc
     3781 gctcgtacac cacgtgcgcg cgaccgacga tcagcggatc gagcttgccg atctgcgcca
     3841 ggtccttgcc ggtgtagggc agcttgtgca gcacgtagcg catggcgttg aggcgggcgc
     3901 gcttcttgca gtcgctcttg atgaccgtcc agggcgagtc ggcggtgtcg gtctcgaaga
     3961 acatggcctc cttggcgcgt gtgtagtcgt cccacttgtc gaggctggcc ttgtcgatgg
     4021 ggctgagctt ccactgcttg agggggtggt tttcgcgctc cttgaagcga cgccgctgct
     4081 cggcacggct gaccgagaac cagaacttga tcaggtggat gccgctgctg accaggtgac
     4141 gttcgaactc gggcgcctga tgcatgaagt cctggtactc acgttcgctg caaaaaccca
     4201 tcacgcgttc gacgccggcg cggttgtacc agctgcggtc gaacagcacg atctcgccat
     4261 gggtgggcag gtgctgcacg tagcgctgga agtaccactg cccgcgctcg atctcgctgg
     4321 gtttttccag ggcgacgaca cgcgctccgc gcggattgag gtgctccatg aagcgcttga
     4381 tggtgccacc cttgccggcg gcgtcgcgcc cttcgaacac gatcaccacg cgctggccgg
     4441 tctccttgac ccagttctgc aacttgagca gttcgacctg cagcttgaac ttctgttgct
     4501 cgtaagtggc gcgacgcagc aggtgttcgt agggatagcc accgtcgcgc cagtccgccg
     4561 ccagttcctc gtcgggattg gacttggttg cgagggtatc gcgtccattg gccttgagca
     4621 ccttgcgcag ggccttgcga tcgtcgggcg aggcgccgtc gagcagcgcg cgcagggcgc
     4681 tggcgcgctc gctcggcgtg ccggcggccg ggtcgttgac caggcgcagc gcggcttgcg
     4741 cctgggcgtc gcgggccccg gcggtggcgt gtgcgaccac gtggtcttcg atggcccgcg
     4801 ccgtcaacga gggcggggta tcgcccaggc gcaccgcgcg caggtcgccg ttggcccgcc
     4861 gggagctggc ggcggtcttg ctggtcttgc ctgttttcgg agcgacgttc ttgcggctgg
     4921 acgcagaacc ctggggctgg tggttggcgg agctcatgga agggtggggc gatgaaggtt
     4981 cgacaagggg acgggccagc cacctgcggc ggcgtgttgc cccaataccc gtactttcca
     5041 tccgggtact gggattcagc ttgaggtagg tcaagaaacg tgagtggcgc gtgccggcgc
     5101 cgtgcagcgt gtcacgctcg catgaccggc cacaccacgc cgttgtcgtt caggatcgcg
     5161 tccagtggct ggtcgtgcgg ctcgggctcg aagtcgtcga cgaagccctg cgtgaaaccc
     5221 agtcccgccg tgtatggccg cggctggagc gccgccagcg tgcggtcgta gaagccgccg
     5281 ccatagccca gccggtagcc gcccggcccg tagccgacgc agggcacgaa cagcagggtg
     5341 ggcacgatca gctcggtgtc cttgggcttg ggaatgccgt aggcgtcttc ttccatcgga
     5401 cagccggggt accagacgtg gaacgtcagc gtcttgtgca ccttgtcgat caccggcagt
     5461 ccgatgcggc ggcgttgcgg ttcgtccatc aactcgccgt cttccttcca gcggtgcagg
     5521 gcgggcaggg ggtcgaactc gcccttgatc ggccagtagg cgcccaccac cgtgtccggg
     5581 cggcccagca gccagatgcg catgatgtcc tgcagcgccg cggcccgcgc cagccggtcc
     5641 tccagatgca ggcgctcgcg caccaggcgt tcgcgcaggg cttttttgtc gaacttgctc
     5701 ataatcggtc gatgcgttgg ccaatgattc tgacatcctc acgcgccgca cgggctgggg
     5761 ccggcctggc gctgctgggc atgacggtgg cctgggcgca accggcggcg gcgccgcagc
     5821 cgccggagcc gaccgccgcc tcgtcgcagg ctgccgacgc cagcccggtg gtgccgatgc
     5881 tggccctgcc cgccgacgcg gcggcgcgcg atgcggtcct gctcgacatg cgcgacgccc
     5941 accggcgcgg cgacaaggcg cggctgacgg ccctgctgcc ccaggcgcgc ggccatgcgc
     6001 tggagccctg ggccgcctac tgggaactca aggcgcgcct ggaggacgcg gccgcatccg
     6061 aggtgcagga tttcctggcc cgctatgccg gcagctacca ggaagaccgc ctgcgcaacg
     6121 actggctgct gctgctgggc aagcggcgcg actgggcggc gtttcgcgcc gaatacccgc
     6181 gctaccgcat gcgcgacgac cgcgaactgc gctgccacgc ggtgtcggcc gacctcgccg
     6241 ccggcctggc gcccggcccg caggcggtcg agcaggtgcg gcgcgactgg ctggcgcagc
     6301 gcgacgccga cgacggctgc ctgtacgccg ccggcctgct gcaccagacc gggctgctgc
     6361 cctaccaggt gctgtggaag aaggcccgcc tggcgatgga ggccaaccgg ccggcgctgg
     6421 cacgcgccgc cgtggcgctg gacgcgcccg acgccgccgc cgagctggcc agcatcgccc
     6481 agagcgcgcc gcgctacctg ggcgggcgcg ccagcagcgc cacgcacaag cggcaggagc
     6541 tggccacgct ggcgctgatc cgactggcca cgcaggctcc cgaccaggcc gcgcagcagc
     6601 tggaagacaa gtgggccgcg ctgctggacc gcgaggagcg caactggctg tggggcgtga
     6661 tcggcaagtg gtcggcgctg gcgccgtccg agcgcgcgca cggctacttc agccgcgtca
     6721 cgcgcgaggg cgacctgagc gacgagctgc tggcctggaa ggtgcgcgcc gccctgcgcc
     6781 agggcgactg gcgcgccgtg ctgcgcagcg tcgacgccat gcaggacgcg gcggccgatg
     6841 atggcgcctg gctgtactgg cgagcgcgtg cccagcaggc gctggcgcgc cacgacgacg
     6901 agcgcgccgc cgcgcgcgcc ggcttcgaac gcctgacggc ccaggtcggg cgccagggct
     6961 tctacgaaaa gctggcgctg gaggcgctgc aacggcccat cgtggcgccg cccgccccgg
     7021 cgccgctgac cgaggccgag cgcgaagcgg cgcgtcgcca ccccggcctc ggccgcgcgt
     7081 tgctggccat cgccctgggc ctgcgcggcg aaggcgtgcg cgaatggaat tactggacca
     7141 acctgcaccg cccgggcggc atgggcgagc gcgagctgta cgctgccgcc gatctggcct
     7201 gcgcgcgcca agtctgggac cgctgcatca acaccagcga gcgcacgcgc agcttcgccg
     7261 acttcgggca gcgctttccg accccgcacc gcgaagccgt gcagcgccag gcgcaggcca
     7321 tcgggctgga cgccgcctat gtgtatggcc tgatccggca ggaaagccgc ttcgtgacgg
     7381 acgcgcgctc gggcgtcggc gcctcgggcc tgatgcaggt catgcccgcc accgcgcgct
     7441 ggacggcgcg caggatcggc ctgagcggct tcgtgcccgc gcagatcaac gacctggaca
     7501 ccaacatcct gatcggcacc agctacctga agctggcgct ggacgacttt cagggctcgc
     7561 tgccgctggc cgccgccgcc tacaacgccg gccccggccg accgcgcgcc tggcgcaacg
     7621 gcccgctgct ggacggcgcg gtgtgggccg aaaacatccc cttcaacgaa acccgcgact
     7681 acgtcaagaa agtgctttcc aacaccgtcg actatgccgg cctgctcacc ggacagccgc
     7741 agtcgctggc ggcgcggctg ggtcgggtgg ggccgctggc gccggggcag gccgacccga
     7801 gcatgggctt gccctgagtc gcgattttag gttcaatcgg gcttcggcgc gcttctgtat
     7861 tgcgccgaca gctatcaaat caggagtcat tcaatgggca agcggatcct ggttctgggc
     7921 ggttcggggt ttgtcggccg ccacctgatc gaacacctgc agcggctggg cgtgcgcgtg
     7981 accgtgccca cgcggcggcg cgacgccgcg ccctggctgt ggagcctgcc gcgtgtcgat
     8041 gtggtgccgg ccgacgtcac cgacccggcg cacctgggcg cgctgctggc cggtcaccag
     8101 gcggtggtca acctgatcgc cgtgctgcac ggcgatgcgc ggcgcttcga gcaggtgcat
     8161 gtggagctgc cgcgccgcat cgccgccgcc tgcgccgcca gcggcgtgcg gcggctggtg
     8221 cacatcagcg ccctgggcgc ggccgccgat ggcccgtcgc gctaccagcg cagcaaggcc
     8281 cagggcgaag ccgtgctgca gcaggccgcc gtcgacggcg cgctggcgct gacgctgctg
     8341 cgccccagcg tcatctacgg cagcggcgac cacctgctga acctgttcgc cgggctgcag
     8401 cggctgtttc cgctgatccc gctggccagc gccgaggcgc gctttcagcc ggtgtgggtg
     8461 cgcgacgtgg cgcaggcgct ggtgcgctgc ctgcaggacg acaccaccgc cgggcgcacc
     8521 tacgagctgt gcgggcccga agtctggacg ctgcgcgagc tggtgcgcgc ggccggcatg
     8581 tgggccggcg tgcgcggcgg gcgcgggcgc ccgatcgtcg gcctgccggc ggcgctggga
     8641 cggctgcagg cgctggccat ggaatgcttg cccggcgaac cgctgatgag ccgcgacaac
     8701 ctcgattcga tgcgggtgga caacgtcgcc agcggccagc tgcccggcct ggccgagctg
     8761 ggcatcacgc cggcgccgct gtcggccgtc ggccccatga tcgtcggtgc cgaccatccg
     8821 gccgcgcgcc tggacgccta ccggcgcagc gccgggcgct gagaatttct ggaagggccc
     8881 cgcatgctca agctctacat cggcaacaag aactattcgt cctggtccat gcgcccctgg
     8941 gtgctgatga agcaggccgg catcgcgttc gaggaagtgc agctgcgctt cgactcgttc
     9001 tcgggcgact cgcagttcaa gcagcgcctg ggccgcatca gcccggccgg caaggtgccg
     9061 gtgctcactg acggcgagct ggtggtgtgg gacacgctgg ccatcgccga gtacctggcc
     9121 gagaaatttc ccgaactgca cctgtggccg cgcagcgccc ccgagcgcgc ccgcgcccgc
     9181 agcgtgtgcg ccgaaatgca cagcggcttt ggcgcgctgc gcagcgcctg cggcatgaac
     9241 atcgaagcgc agctgcccga ggccggcgcc atcatctggc gcgaccggcc cgacgtgcgc
     9301 gccgacgtgg cgcgcatcgt ggagatgtgg caggaactgc tggccatgca cggcgggccg
     9361 atgctgttcg gccgcttcgg catcgccgac gccttctacg ccccggtggt gatgcggctg
     9421 aaaacctacg ccctgccggt gccggacgac gtggccgcct acatggcgcg cctgtgcgcg
     9481 ctgcccggcg tcgccgcctg gatcgaaggc gcgctggcgg agcaggactt cctgccgttc
     9541 gaagaaccgt accgcgcggc ccgaccggcg tcggcctgaa ccggggcgcg ccggcgcccg
     9601 cccggccccg gatttggcag gtcttaaaga ttcatgcaaa atgcagcttt tgacattccg
     9661 atgccgccgg cctcttcgcc gctgccgccg gccagccctc catgacccag cgttccgctc
     9721 ccctcatgtc gatcgatgac cgcgcgcagc ccccggccgc gctgccgggc tggccgctgc
     9781 tgcggctggg ctttcggccc ttctacctgg gcacggcgct gctggcctgc gtgtcgatcc
     9841 cgctgtggat cgcggccttc ctgggccaca tgccgctgcg gctgccgttt ccgatcctgc
     9901 tgtggcacgc gcacgagatg ctgttcggct ttgccgccgg ggtggtgatc ggctttctgc
     9961 tgaccgccgt gcgcgcctgg accgggctgg agacgccgcg cgggccggtg ctgggcctgc
    10021 tggcgctgtt gtggctggca gcgcggctgg cggccgtgct ggcgccgtac ccgctgttcg
    10081 ccgtgctcga tctgctgctg ctgccggcgg tggcgttctt cctgctgcgc gtgctgatcc
    10141 gcgccggcaa ccggcgcaac atcccgctga tttcgctgct gctggcgatg gcgctggcca
    10201 acgcggtgtt ccacctgtcg gtgctggggc tgatcgccgt gcccgccatg tcggccctgt
    10261 acgccgagct ggcgctgatc ctgatggtgg tcagcatcat gaccggccgt gtggtgccca
    10321 tgttcaccaa gaacgtgacg cccggcctgg tgatccaggt gtcgcgccgc ttcgagctga
    10381 cgctgctggc cgtcaccgcc gtcacgctgt cgctgtgggt gctgggtgcg ccggcctggc
    10441 tggtgctgcc cgccagcgcc ctgtctgccg cgctgcacgc gctgcgcctg tggaagtggc
    10501 atccgcgtgt gacgctgcgc cgcccgatcc tgtggatcct gcatttttcc tacgcctgga
    10561 tgccggtcgg ctttgcgctg ctggcgctgg cgcagccggg ctgggtggcg ccgtcgctgg
    10621 cggtgcatgc gttcggcgtc ggcgtgatcg gcgggctgat catcggcatg atcacgcgca
    10681 ccgcgcgcgg ccacaccggc cggccgctgc aggcctcgcg cggcgaggtc gtggcctacg
    10741 cgctggtgat gctggccacc gtgctgcgcg tgctggtgcc ggcggtggtg ccggcctggt
    10801 acgtgcgggc gctggattcg gccgccgggc tgtgggcgct ggcctttgcc atctacctgg
    10861 tgatctatgt gccctggctg acgcgcacgc ggctggatgg caaggacggc tgagcgccgg
    10921 cccccggttt ttcgttccgg gcgccgcggg cgtccgggca ctacccgctt cttcgcagct
    10981 ctttcagaaa ggttgatcca tgagcaccgc caccctcgcc gaaatcgacg tgcgcagcat
    11041 tccaccgtat cagcgccacc agcagatctt tgcccaggtg gacgcgctgc aggtgggcca
    11101 ggcgctgcag atcgtcaacg accacgaccc ggtgccgctg cgccgccaac tggaatcgcg
    11161 ctcgcccggc cagctgcagt gggcctatgt gcaggccggc ccggacctgt ggcgcgtgca
    11221 gatcagcaag gtgcagggcg cggccgacgc cgaaggctcg tgctgctcgg gcggcgcctg
    11281 ttgcggctga ccgggtcggg caagacgcac cggcgcgccg gcgcaagggc tggcgcgctt
    11341 ttttgttgcc gggggcctat agccccaggg ccagctgcgc cgcttcggac atcatggaac
    11401 ggtcccaggg cgggtcgaac accagattga cggtgatttc gcccacgcgc ggcagcgcca
    11461 gcaccttgtc gcacacctcg ttggcgatcg attcgcccat gccgcagccg ggggcggtga
    11521 gggtcatgtc gattaccacg cgcacctggt cgggatcgtc cagcggctcg aacgccagcc
    11581 gatagaccag gcccaggtcg gcgatgttga ccgggatttc cgggtcgtag caggtgcgca
    11641 gcgtgtccca caccagcggc tccagctcct cgatcggcag gtccttgggc acggccaggg
    11701 cttcgggcgg ctgcttgccg atggcgtcgg cgtcctggcc cttcaggcgc atcagctgac
    11761 cctcgaccag cagggtgaag gagcccccca gcgcctgcgt gatctgcgcc agctggccag
    11821 gctccagctc gaccgggtgg ccgctgggaa tggtttccac cgtgacgtgg cggcgcacca
    11881 gcacgtcctc gcggtggcgg cggttcatca tggcagcgac tcctcggtgc cgccggcgtc
    11941 ctgctggccg gcaatggcgt gcatcagcgc gtgccagccc agcagcgcgc acttgatgcg
    12001 gctggggtac tggcgcacgc cggccaggct gaccagcttg cccagcgggg cttcgtcggg
    12061 gtcttcctcg cccgtcagca cggcgcgaaa gtgctgctgc agcgcctgcg cggcggcgat
    12121 gtcgcggccc ttgacggcct cggtcatcat cgagctggag gccatgcaga tggcgcagcc
    12181 ctggccggaa aagcggatgt cctgcacccg ctcgccgtcc agcagcagct cgaccgccac
    12241 gcggtcgccg caggacgggt tggtgccctg ggcctggtgg gtgggctgcg gcaggtggcc
    12301 gaagttgcgc ggcgcgcgct tgtgctccag caccacttcc tgatagagat cgttttcggc
    12361 gctgctcatc gcaacatctc cagggcgcgc gccacggcgg cgatcaggcg ctcgacttcc
    12421 tcggcgctgt tgtagagggc gaacgaggcg cgcgtggtcg gccccagtcc gaagtggtcg
    12481 tgcagcggct gggcgcagtg gtggccggtg cgcacggcga tgccgcgctc gtccagcaag
    12541 gtgccgatgt cgtgcgggtg cacctggtcg gccacgaagg acaccagcgc cgcctgcgtg
    12601 gcgtgcggcg ccagcacggt gacgccgggc agggccgaca ggccggccac cgcctgcgcg
    12661 cgcagcgcgt gcaggtggcc gtcgatggcg gcgcgcccga tccggtcgat gaacgtggcc
    12721 gccgccgcca ggccgaccgc gccggccacg ttgggggtgc cgccttccag gcgcgccggc
    12781 agcgcggcgt agatggcgcg ctcgtagctc acgtcctcca ccatgtcgcc gcccaggcgc
    12841 agcgggtgga gccgctccag cgcgtcgcgc cggccggcca gcacgccgat gcccatgggg
    12901 ccgtacatct tgtggccgga aaaggccatg aagtcgcacg ccagcgcgct cagcgccggc
    12961 atgccgtggc ccaccgcctg ggcggcgtcc agcacggtca gcgcgccggc ctggcgcgcc
    13021 atggccagca tggcttcgta gggcgggcgc tcgccggtgg cgttggcgct ggcggtgatg
    13081 gccagcacgc gcgtgcgcgg cgtcagcagc gcggccaggg cgtcctgctg caggcggccc
    13141 tgggcgtcgg ggcgcagcac caccagctcg gcgccgctca tgcgggcggc gtgctgccac
    13201 ggcaccaggt tggcgtggtg ttccagtccg gtgctgatga tgcggtcgcc cgggcgcagc
    13261 caggcctggc cggggccggc gccccacagg ccctgggcca ccaggttgag tgactcggtg
    13321 gtgccgctgg tgaacaccag ctcgtgcccg gcgtcggcgc cgatgaagcg cttgagcgtg
    13381 gcgcgcgcct gttcgaaggc gtcggtggcg cgctggctga gcgtgtgcac gccgcggtgg
    13441 atgttggcgc gctcgtgcgt ctcgaagcgg cgcatcgcct ccatcaccgc cagcggcatg
    13501 tgggtggtgg cggcgttgtc caggtaggcc agcgggtggc cgtggatctg ctggctcagc
    13561 accggaaact gggaccgcca ggccgccagc ggcggtggcg cggccgccgc gcggtcggcc
    13621 tcaggcaggg tgaggggggg ctggtggttt ggcatcaaaa agggctctgg cgctgatgtg
    13681 gattgcgcaa gcagctatca aaaacatagt cttcaggcgg gggtttcggc gcgctgccgg
    13741 gcctgccgcg ccatgcgctg cgtcagccag ccggtggcca gccagtcgct cagcagttcg
    13801 gtgtcgccga ggctgcgctc cagcacggcg cgcgccatgc cttcggtgat cagggcgcgc
    13861 gcgctggctt cgtcgaggcc gcgctggcgg gcgtagaaca gcgcgtcggc gggcagcgcg
    13921 ccccaggtgg cgccgtgcgc ggcctgcacg ttgtcgtgca ggatttccag gtgcgggcgc
    13981 aggatcaggc gcggctgacc gccgagcggt atgccggaca ggcgctggcg cacctcggcc
    14041 tcgtcggcgc cggcggcgat gcgggtgtag gcgttgctga cggcgcgcgc gccgccctgg
    14101 gccagcacca gcgattcgac ggcgctggcg ctgtgcgccg ccgccaggtc gctgtgcgcc
    14161 tggtggtcga gctgctggtc gtccagcagc agcagggcgg cctgctgggc gcgcgcgccg
    14221 cgtccgctca gttggacggt gctgcgctgc aggtggtagg cgctgccggt ggccaccagc
    14281 gcctgcgcgt agtgcgccgc cggcgccagg ctgacgtgca ggtggtgggc gatctggtcc
    14341 tgcgcctggg gcgccaccac gcgcaggtgc tgcaggtgcg cgccggccgc caggcggatg
    14401 tggccgtgca ggttctgggt cacgggatgg gcgccgtcgg cggcggtgcg ttcgtgggtt
    14461 tccagcagca cgcagtgcac gccggcgtcg acctcgagca ccagcagcgg cgcttcggcg
    14521 cgctggtgcg gctggtggcg cagctgcagc accaccgggg cggcgggctg gccgggcgtg
    14581 gcgctgaggc gcaggcgcag gccctgacgg cacagcgccc ggtgcgccca gacgaagggc
    14641 gcggcctcgc cgtcgccggg cggcggcagg ccggccagca gctcggcgcg ctgggccggg
    14701 tcgagggcgt cgagccagcg cgcgtccacg ccgtgggcgc cgccgtgacc cagcgggtgc
    14761 agcgtccagc cggcgccggc cagcgccggg gcgttgccgt cgtcggcgtc ggcgtccggc
    14821 tggcccagcc agtgcgccag ctcgggcggc ggcaggtggt ggaaggcttc gctgcggcgc
    14881 gcgatccagc cctggcgctg caggtgttgg cgcgcggcca ggcgttcggc ttgcgacggg
    14941 ttcatgtcag gcccccgccg gcgccgtgcg cgaggcaaag ccggtttcgg cgatgtggcg
    15001 tgccaggccc aggtcgccgg tttcggcgat gcagccctga tcgagccgca gcacggcgtc
    15061 gggttcgagc tgctcgatca tctgcaggta gtgcgacacg acgatgaagg ccgtgccctg
    15121 cgcgcgctgg cgccgcacct gctcgaccac ggcgcgcacg ccgtcgacgt ccatgccgga
    15181 gtcgatttcg tccagcagcg ccaggcgcgg ctgcagcagc gccagctgca gcagctcgtt
    15241 gcgcttgcgc tcgccgccgg aaaagccctc gttgaccggg cggcccagca tggcttcggg
    15301 cagccccagg gccttggcgg cctgcttggc gctggtgagg aagtcgaagg cgtcgagcgg
    15361 cggctggccg cgcgattcgc gctgcgcgtt gagcgcggta cggatgaaca ggttgttctt
    15421 gacgcccggc acgtcgggcg gcgactggaa cgacaggaac aggccggcgc gcgcgcgctc
    15481 gtgcgccggc agcgccagca ggtccttccc ggccagcagc gcctggccgc ccagcacctg
    15541 gtaggccggg tggccggcca gcgccaggcc cagcgtgctt ttgccgctgc cgttggcgcc
    15601 catcagcacg accaggctgc cggcgttgac gtcgagcgag accgagtgca gcacggtgcg
    15661 ctcgcccacc tggacgcgca gatcgcgcac ctgcagcagg ggttcggggg aggtcatcag
    15721 ggaatccttt tgctaatatt tcgatagctg ctggcgcttg ctgggtaagc gctggcggcc
    15781 tgtttgatgg ctatccgttc agcccaccgc gccttccagc gacaccgcca gcagcgcctt
    15841 ggcttcgagc gcgaactcca tcggcagctc ttccagcacg gcctggcaga agccgccgac
    15901 gatcagcgcc gtggcctcct gggcgtcgat gccgcgctgt tcgcagtaga acagccgctc
    15961 ggccgagatg cgggtggtgg tagcctcgtg ttccaccacg gcgtcggcgc gcccgctgtc
    16021 gacgtagggg aaggtgtggg cgccgcagtg cgggccgatc agcagcgaat cgcactgggt
    16081 gtggttgcgc gcgccttcgg cccctgggtt gatgcgcacc aggccgcggt aggcgttgtg
    16141 cgcgtggccg gcgctgatgc ccttggagac gatgcggctg gtggtgttct tgcccaggtg
    16201 gatcatcttg gtgccggtgt cggcctgctg gcggtggttg gagacggcga tggagaagaa
    16261 ttcgccgctc gaaccctcgc cgcgcagcac gcaggagggg tacttccagg tgatggccga
    16321 gccggtctcg atctgcgtcc aggcgatgcg cgagcgcttg ccggccgcca ggccgcgctt
    16381 ggtgacgaag ttgtagatgc cgccgacgcc gtgctcgtcg cccgggtacc agttctgcac
    16441 cgtggagtac ttgatgaagg cgtcgtcgtg ggcgatcagc tcgaccaccg cggcgtgcag
    16501 ctggttttcg tcgcgctgcg gcgcggtgca gccttccagg tagctgaccg agctgccctc
    16561 ttcggcaatg atgagggtgc gctcgaactg accggtgttc tgggcgttga tgcgaaagta
    16621 cgacgacagc tccatcgggc acttgacgcc cttggggatg tagacaaacg agccgtcgga
    16681 aaacaccgcc gagttgagcg cggcgtagaa gttgtcgccc tgcggcacca ccgtgcccag
    16741 gtatttttcg accagctcgg ggtggttctg caccgcgtgc gagaacgaac agaaaatcac
    16801 gcccacgtcg gccagctgct tgcggaaggt ggtgcccacc gagaccgagt cgaacaccgc
    16861 atccaccgcc acgccggcca gcgccgcgcg ctcgtgcagc ggcacgccga gcttttcgta
    16921 ggtctcgagc agcttggggt cgacctcgtc gaggctcttg ggcttgtcct tcatggactt
    16981 gggcgccgag tagtaggaca gggcctggaa gtcgatcggc gcgatcttca ggcgcgccca
    17041 gtcgggcagc gccatggtct gccagcgccg gaacgcggcc aggcgccagt cgagcaggaa
    17101 ctgcggctcc ttcttcttgc gcgaaatggc gcggatggtg ccctcgtcca ggccgggcgg
    17161 caggctgtcg gactcgatgt cggtcacgaa gccgtgctgg tagggccgct gcagcgcctg
    17221 ctcgagcggc gcttcctgcg gcttgtccgc cagggcctgc gcggtgctga tgggttcagg
    17281 tgatgccatg ggcgctcatc cgtttaaatg tacatatcgt atgcatcttt gaccgactcg
    17341 gcaaaccggc tgcgccgtgc ctgcacctga gatggggaca ttgtccgggc agggcggcgc
    17401 ggccggggcg ccgcactgct cctacacttg ccccatgcag atctacatgg tgggtggcgc
    17461 cgtgcgggac cgcttgctcg gcctgccggt gcaggatcac gactgggtgg tggtgggcgc
    17521 cacgcccgaa gacatggtgg cccgcggctt cgtgccggta gggcgggatt ttcccgtttt
    17581 tctgcacccc tcgtcgcatg aggaatatgc gctggcgcgc accgagcgca agagcggccg
    17641 tggctacaag ggtttcaccg tcttcaccag ccccgaggtg cgcatcgaag acgacctggc
    17701 gcgccgcgac cttactatca atgcaatagc tgttcgcgca ggccaggcaa gcgctggagc
    17761 cacatttgac catgaagacg gggtgatcga tccgtttggc ggccggcgcg acctgcaggc
    17821 gcgtgtgctg cgccacgtca ccgacgcctt ccgtgaagac ccggtgcgca tcctgcgcgt
    17881 ggcgcgtttt gcggcgcgct ttcacgactt cagcgtggcg cccgagacgc gggcgctgat
    17941 gcgccagatg gtggccgagg gcgaagccga ccacctggtg cccgagcgcg tgtggcagga
    18001 gctggcgcgc ggcctgatgg aaagccagcc ggcgcgcatg ttcgaggtgc tgcgcgactg
    18061 tggcgcgctg gccgtggtgc tgcccgagct cgaccgcctg tggggcgtgc cgcagaccgc
    18121 ctcgcaccac cccgagatcg acaccggcgt gcacgtgatg ctggtgctgc agatggccgc
    18181 gcgcctgcag gcgccgctgg cggtgcgctg ggcctgcctg atgcacgacc tgggcaaggg
    18241 cgaaacaccg gccgacctgc tgccgcgcca ccttggccac gagcagcgca gcgcccgcct
    18301 gctgcgcgcc gtcggccagc gcctgcgcgt gcccaccgac tgcaccgagc tggccgaggt
    18361 ggtggcgcgc gagcacggcc acatccaccg cagcgccgag ctgggcgccg ccgccctgct
    18421 gcggctgctg cagcgctgcg acgcgctgcg caagcccgag cgctttgccc aggtgctgct
    18481 ggcctgcgaa tgcgacgcgc gcgggcggct gggcttcgag cacagcgcgt acccgcagcg
    18541 tgcgcgcctg caggcggcgc tgggcgcggc gcagtcggtg gcgacggcgg ggctggcgca
    18601 ggcggcgcag gccgccggcg cgcgcggcgc cgacatcggc caggcggtgg cgcaggcgcg
    18661 cacacgggcg ctgcaggcct ggctggacgg gtcggccccg gccgcggtct gatcggcgcc
    18721 gccgattgct acgaattgga tagctgttgg cgcagtctgg gtaagcgcca ggacttgttt
    18781 tttctgtgaa cggcgggcgc gccgacggcg ccggccgggc gccgataatc acgccccatg
    18841 gcccgcccga tctctgccgc ccacggtctg cgccgcgacg aaatcctcga cgtcgcggcc
    18901 gactgctttg cgcgccagag ctacccggcc accagcatga acgacatcgc cgccgcctgc
    18961 ggcacctcca aggcgcggct gtaccactat tacgacagca aggacgccat cctttttgag
    19021 ctgctggacc gctacaccca gcggctgctg gtcatcatcg gccaggtgca ggccacggca
    19081 caccgccagg cgctggacga gcgcgccacc ctgcacgcgc tgatccgcgc ctttctgcgc
    19141 gaatacgaaa gttctgccac gcgtcatgcg gcgctgctgg ccagcaccca gttcctgggc
    19201 gaaacccagc gcggcgtgat cctggatcgc cagcgcgacg tggtgtcggc cgtcacgcgc
    19261 tttctgcgcc gcgcctaccc cgagcgggtg gacgcgcgca accagaccgc catcaccatg
    19321 acgctgttcg gcatgatcaa ctggaccttc acctggctgc gccccggcgg gtcgctgagc
    19381 tacgacgcct tcgccgacga ggtgattgcg ctgctggagc gcgggctgtc ttcacccctg
    19441 aaccctcact cacgttcatg aacccgatcg atatcgccgt catggctgcc ggcaagggca
    19501 cgcgcatgaa aagccgcacc cccaaggtgc tgcagaaact ggccggtcgg ccgctgctgg
    19561 gccatgtgct ggacaccgcc gcccagctgc gggcccgctc ggtggtcgtc atcaccggcc
    19621 acggtgctac tgaagttgaa gcgtttggcg caagcctggc gggcgccgga gccggatttg
    19681 acttgcaatt cgcacgtcag gagccgcagc tgggcaccgg ccacgccatg cagcaggcca
    19741 tgccgcggct ggccgacgat ggtctggtgg tggtgctgtc gggcgacgtg ccgctgaccg
    19801 aagcggccac cctgcaggcc ctggtcgacg ccgcccaggg cgagcggctg gcgctgctca
    19861 gcgtgacgct ggccgacccc agcggctacg gccgcatcgt gcgcggcgcc ggcggcgccg
    19921 tgcagcgcat cgtcgagcac aaggacgcca gcgcggccga gcgcgccatc ggcgagatct
    19981 acagcggcat catggcggtg ccggcgcgcc tgctgcgcgg ctggctgaca cggctgacca
    20041 accacaacgc ccagggcgag tactacctga ccgacgtggt ggccatggcc gtggccgacg
    20101 gcgtgccggt ggtggcgcac tgcatccagg acgcgttgca ggtggccggc gtcaacagcc
    20161 cgctgcaact ggccgagctg gagcgcgccc accagctgcg ccaggcgcgg gcgctgatgg
    20221 aagccggcgt gcgcctggcc gacccggcgc gcttcgacct gcgcgacgac gcccgccgcg
    20281 gcgtgcgcgc cgaactgcgc tgcgggccgg acgtggacat cgacgtcggc tgcatcttca
    20341 ccgggcgggt cgagatcggc gagggcgcgc agatcggcgc ccactgctgc atcgccaacg
    20401 cccgcatcga ggcgggggcg gtgatccacc cttacaccca catcgacggc gaagccgccg
    20461 gcgtgcacgt cggcccgggc gccctggtgg ggccgttcgc ccgcctgcgc cccggcgccg
    20521 acctggggcc cgaggtgcac atcggcaact tcgtcgaagt caagaactcc agcctggccg
    20581 ccggcgccaa ggccaaccac ctggcctatc tgggcgacgc cgtggtgggc gagcgcgtca
    20641 actacggcgc cggcagcatc accgccaact acgacggcgc ccgcaagcac cgcacggtga
    20701 tcgaggccga cgtgcatgtg ggcagcaact gcgtgctggt ggcgccggtg accatcggcg
    20761 ccggcggcac cgtgggcggc ggctccacca tcacgcgcga taccccgccg ggcgggctga
    20821 gcgtggcgcg cgggcgccag gcccacatcg ccaactggca gcgcccggcc aagccgcaag
    20881 gtggcggctg ag
//
LOCUS       DAOQTZ010000046        19226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3313_length_19226_cov_13.152418, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000046 DAOQTZ010000000
VERSION     DAOQTZ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 19226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19226
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3313_length_19226_cov_13.152418"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..77)
                     /locus_tag="PKN51_07210"
     tRNA            complement(1..77)
                     /locus_tag="PKN51_07210"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Pro,seq:tgg)
     gene            complement(218..1477)
                     /locus_tag="PKN51_07215"
     CDS             complement(218..1477)
                     /locus_tag="PKN51_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT84925.1"
                     /translation="MEFDYSPKVQDMQARLLAFMDQHIYPNEARFYEEVAANRAKGNA
                     WIPTRIIEELKPKAREQGLWNLFLPKSKRAPEGLSNLEYAPLCEIMGRVSWAPEVFNC
                     SAPDTGNMETIERYGSEANKDQWLEPLLRGEIRSAFLMTEPAVASSDATNIECRIQRD
                     GDHYVINGRKWWSSGAGDPRCAIYVVMGKTDPDAPRHSQQAMILVPANTPGVKVIRPL
                     TVFGMDDAPHGHMEIELKDVRVPAGNLLWGEGRGFAIAQGRLGPGRIHHCMRSIGAAE
                     RALELMCQRLNHRVAFGRRISEQGVWRERIAESRCMIDQARLLTLKAAYMMDTVGNKV
                     AQAEIAMIKVVAPNVSTKVIDWAIQAHGGGGVSNDFPLASMYAHQRTLRLADGPDEVH
                     RNAIAKLELAKHMPGAKAVEMPVTRGS"
     gene            complement(1538..2623)
                     /locus_tag="PKN51_07220"
     CDS             complement(1538..2623)
                     /locus_tag="PKN51_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNT84926.1"
                     /translation="MSHTDQFVGTRPVSGAHAFDTAALGAWMTQHVEDFAGPLGVEMF
                     KGGQSNPTYKLITPGRTYVMRAKPGPVAKLLPSAHAIEREFAVMRGLASTSVPVARMH
                     ALCEDESVIGRAFYIMEYVEGRVLWDQTLPGMTPAERGAIYDEMNRVIAALHKVDYAA
                     RGLAGYGKPGNYFERQIGRWSKQYQASVTQPITEMDRLIEWLPTHMPASARDDGLTSI
                     VHGDYRLDNVIFHPSEPRILAVLDWELSTLGHPLADFSYHCMAWHIPPGTFRGIGGVD
                     LASLGIPSEQDYIHRYCERTGLATPEQLAPDWNFYLAYNMFRIAAILQGIAKRVEAGT
                     ASSAQARASGAGARPMAELAWSFAQKA"
     gene            complement(2625..>3247)
                     /locus_tag="PKN51_07225"
     CDS             complement(2625..>3247)
                     /locus_tag="PKN51_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT84927.1"
                     /translation="CAFVKRYPDGELISARGEPPEQWIACAKGAVRVSSTTLTGKQIT
                     LTYVEPGVWFGDVAMFDGDRRTHDAYAHGVTTILCVARADFHKILAQHTELYEALLQL
                     QARRIRLLFGQVEDLNTLPLRARLAKQLSHLVRSYGVPSLSSNDEIRIALQLAQEELA
                     QLLGASRQRVNQELKTMEREGVIRVEPTGLVVCDRDALLRISEGEQ"
     assembly_gap    3248..3347
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3348..3499)
                     /locus_tag="PKN51_07230"
     CDS             complement(<3348..3499)
                     /locus_tag="PKN51_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT84928.1"
                     /translation="MDEPILTMEEREAINSGRWFSSLSPSLRHDILRCAFVKRYPDGE
                     LISARG"
     gene            complement(3584..4363)
                     /locus_tag="PKN51_07235"
     CDS             complement(3584..4363)
                     /locus_tag="PKN51_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNT84929.1"
                     /translation="MTAELRSTFEGSTMVLTISNPEHRNALGPEMYAAGVEALSAAES
                     NPEVRCVVLTGEGQVFCAGGNLQRLQANRQRPPEVQAQSIERLHDWVEAIRTFPKPVI
                     AAVEGPAAGAGFSLALACDLIVAARNAVFVMAYSSVGLSPDGGGSWSLARALPRQLVN
                     QLLMGGERIGAQRLHELGVVGRLCDAGQALGSALDWAGQLGARAPNVMASIKELVGEA
                     DRNDLSSHLALERDHFVRNLHHPNAGIGIAAFLAKQTPRYE"
     gene            complement(4659..6182)
                     /locus_tag="PKN51_07240"
     CDS             complement(4659..6182)
                     /locus_tag="PKN51_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNT84930.1"
                     /translation="MNTPVFSRVGIVGTGAMGRGIAQIAAQAGSTVRLYDTQPDAVAK
                     AREALSAQWDKLVHKGRLTREQADSHVSRVPPAATLADLADCDLVIEAIVERLDVKKT
                     LFAELEGIVRPDAILATNTSSLSVTAIAAGLKHPGRLAGYHFFNPVPLMKVVEVIAGL
                     KTDAATGQRLTDYARQMGHTPVQAADTPGFIVNHAGRGYGTEALRIVGEGVTDFVTVD
                     RILRDQVGFRLGPFELFDLTALDVSHPVMESIYRQYFDEPRYRPSVITAQRLAGGVLG
                     RKSGEGFYRYLGGSAQVAPEPAAPQVAELPPVWVSTRAARRAELYQLLKDLGARIETG
                     QSPSPQALTLVAPLGFDVTTVAVVERLDPARTVGIDLLIEDAATRRRVLATNPATRSD
                     MRDAAHALFARDGKPVSVIHDSGGFVTQRVVAHIVNIAADMCQQRVCSPQDLETAVTL
                     GLGYPLGPLAMGDRWGPANMLEVLFNLQTVYGDPRYRPSPWLRRRGAIGLSLLHEEA"
     gene            complement(6316..7323)
                     /locus_tag="PKN51_07245"
     CDS             complement(6316..7323)
                     /locus_tag="PKN51_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HNT84931.1"
                     /translation="MNIVILDDYQDAVRKLACASRLEPYPAKVYTNTVKGLGQLSVRL
                     RDAEVLVLIRERTQITRQLIEKLPRLKMISQTGRVGSHIDVAACTERGIAVAEGVGSP
                     VAPAELTWALIMAAMRRLPQYIGNLKHGAWQQSGLKSASMPPNFGLGQVLRGKTLGIW
                     GYGRIGQIVAGYGRAFGMQVLVWGSSDSMTRARLDGHQVAPSRAEFFALSDVLSLHLR
                     LVEATHAIVRLEDLSRMKPTALLVNTSRAELIEHDALVSALNRGRPGMAAVDVFESEP
                     ILQGHALLRLENCICTPHIGYVEQDSYELYFGAAFDNVVNYLRGTPTQIVNPGALQMR
                     R"
     gene            complement(7422..8585)
                     /locus_tag="PKN51_07250"
     CDS             complement(7422..8585)
                     /locus_tag="PKN51_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT84932.1"
                     /translation="MPIETTTDDSYLASAGRRQLLRGAGSLSLLAAGGLFGGRVAVAA
                     TIDKPADVPVVDRVAIRVVTDSYHHAFEPPRTLGNLQVQRLGFDVKPGRPPRRTLQNE
                     WGLSLHIESGRGDEVRQVLLDFAYTPEALLNNLLLCGIDVSKLDAIALSHGHYDHFGG
                     MVGFLDAHKSKLKLGLPFYLGSEECFCSRELNVPGDAGSFGALDREAIRQSGMTMTFA
                     QRPSILASHGFTTGVIPSSSFERVLAPTRMTPGIQAGLGCAPEGLSPAKQSLTAAVPD
                     DFEHEQAIVYQLKGRGLVVMTSCGHRGIVNSVRRAMQVAGTDKVHAILGGFHLAPHTP
                     EYQRQTLAELKAINPDFLIPMHCSGETFISMAQSEMRDRFVRSSTGTRFVFSA"
     gene            complement(8744..9133)
                     /locus_tag="PKN51_07255"
     CDS             complement(8744..9133)
                     /locus_tag="PKN51_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84933.1"
                     /translation="MARFTTHQPTAATVLCLDDYRVPSYRRPRRPGSAGSSLVRPAPD
                     NAWSGPARQRALQPGAGGLPLHPDPAPTAAPCSAPPVRTLRLRDPQAPDAPARLRISG
                     RLIDVCAELERLAAAELANSPPQPRCA"
     gene            complement(9259..9924)
                     /gene="lexA"
                     /locus_tag="PKN51_07260"
     CDS             complement(9259..9924)
                     /gene="lexA"
                     /locus_tag="PKN51_07260"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNT84934.1"
                     /translation="MSDMPKLTARQQQILQLVQHAIARTGAPPTRAEIAAELGFKSAN
                     AAEEHLQALARKGVIELVSGTSRGIRLKGAARSRLAGDAFSLTLPGMAQLALPLVGRV
                     AAGSPILAQEHVEQTYHLEPTLFAQRPDYLLRVRGMSMQGAGILDGDLLAVHATREAR
                     NGQIVVARLGDDVTVKRFHRHQHQIELHAENPDYPTIVVQPGEPFEIEGLAVGLIRNS
                     MLM"
     gene            10348..10749
                     /locus_tag="PKN51_07265"
     CDS             10348..10749
                     /locus_tag="PKN51_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNT84935.1"
                     /translation="MLSAINAVRATARSCGGTAMPAAAALSWNALLAHAAAAHSTDMA
                     THNFFSHTGSNGSNMAQRVDAAGYVWQALAENIAAGNASVAATVSQWVNSPGHCQAMM
                     SATYVHVGGACRYNASTQYGYYWTIDLGKPF"
     gene            complement(10887..11543)
                     /gene="adk"
                     /locus_tag="PKN51_07270"
     CDS             complement(10887..11543)
                     /gene="adk"
                     /locus_tag="PKN51_07270"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNT84936.1"
                     /translation="MRLILLGAPGAGKGTQAAFICQKYGIPQISTGDMLRAAVKAGTP
                     LGLQAKGVMESGGLVSDELIINLVKERIAQPDCASGFLFDGFPRTIPQAEAMKAAGVK
                     LDYVLEIDVPFDAIIERMSGRRSHPASGRTYHVKFNPPKVEGKDDVTGEPLVQRDDDK
                     EETVRKRLDVYSQQTRPLVDYYGDWARREPAAAPKYRQISGTGTVEEITARALAALAS
                     "
     gene            complement(11913..12680)
                     /gene="kdsB"
                     /locus_tag="PKN51_07275"
     CDS             complement(11913..12680)
                     /gene="kdsB"
                     /locus_tag="PKN51_07275"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNT84937.1"
                     /translation="MSFTVLIPARLASTRLPNKPLADIAGKPMIVRVAERAERSGAAQ
                     VVVAADAPEILHACAAHGVRAIATRADHASGSDRLAEACALLGLDGDDVVVNVQGDEP
                     LIDPALIDAVAALLSSSPVARMGTAAHAIENVADFLNPNVVKVVTDAQGLALTFSRAP
                     LPWWRDGFAGGVTALPAQPAPLRHIGLYSYRAGFLRAFASLAPAPLEQCEALEQLRAL
                     WHGHRIAVHVTAQAPGPGVDTPEDLARVRALLASGAG"
     gene            complement(12677..12889)
                     /locus_tag="PKN51_07280"
     CDS             complement(12677..12889)
                     /locus_tag="PKN51_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNT84938.1"
                     /translation="MDPKLLELLVCPVTKGPLDHDRDKGELISRSARLAYPIRDGIPI
                     MLEAEARALSDDELAALPPRTPLVGG"
     gene            complement(12900..13232)
                     /locus_tag="PKN51_07285"
     CDS             complement(12900..13232)
                     /locus_tag="PKN51_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNT84939.1"
                     /translation="MKNHLFLLGAIVAEVVATSALKASDGLSRGGPTALVVIGYALAF
                     WLLSLTLRTIPVGIAYATWSGLGMVLIALAGWWLYGQRLDGWALLGMALVVAGVLVMN
                     LLSPSSAH"
     gene            complement(13229..14332)
                     /gene="lpxK"
                     /locus_tag="PKN51_07290"
     CDS             complement(13229..14332)
                     /gene="lpxK"
                     /locus_tag="PKN51_07290"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNT84940.1"
                     /translation="MLRAFIERRLRAAWQRRGVLARLLWPLSRLYGALAAHRRRRYLS
                     GALPVARLPAPVIVVGNVIVGGAGKTPTTLALVAHLQARGWRPGIVSRGHGRASDDCR
                     AVQPDSDPREVGDEPLLLRRRAGVPVFVARQRAQAGRALLAAHPDTDVLVCDDGLQHL
                     ALARDLEVVVFDARGCGNHWLLPAGPLREPWPRSPAREPGPGSPVDPREPGPRPAELV
                     LWTEPPAPDQAPAPAAFGATRALAEHALRADGGRQPLADLRGQPLTALAGIARPEAFF
                     AMLRAAGLTLTDTLALPDHYDFDSCLRLLDGRQNLICTEKDAVKLWRHRPDALAVPLR
                     LQVPAAFFAALDERLAARGYHPASRADRPTAAP"
     gene            complement(14550..14960)
                     /locus_tag="PKN51_07295"
     CDS             complement(14550..14960)
                     /locus_tag="PKN51_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNT84941.1"
                     /translation="MKFRPHPPEEPEVNLIPFIDVLLVILIFLMLTTTYSKFTELQVT
                     LPVADADASRERPKEIVVAVAADGRYAVNRQPLEARSVDAVARALRALAGKDSVLIIS
                     ADATAPVQAVVTVMDAARREGLTQITFAAQSGGR"
     gene            complement(14957..15616)
                     /locus_tag="PKN51_07300"
     CDS             complement(14957..15616)
                     /locus_tag="PKN51_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT84942.1"
                     /translation="MLSIIQAAGWPIWPLILCSILALAFAIERFVQLQSRRVLGAQLV
                     DEAIGVSRESVPPPDMVRQLELHSALGEVLAAGWRAINANPRCSPEEMRAAMEAAGRQ
                     VAHRLERYLPALATIASAAPLLGLLGTVIGMIEIFGSQAPAGSLSGGNPAQLAHGISV
                     ALYNTAFGLIVAIPALILWRYFRARVDGYVLELELAAERFARHLEPLCPGRAADAGAG
                     R"
     gene            15992..17401
                     /gene="xseA"
                     /locus_tag="PKN51_07305"
     CDS             15992..17401
                     /gene="xseA"
                     /locus_tag="PKN51_07305"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNT84943.1"
                     /translation="MNTGFEPAAPALAPRVWAVGALARAVADLLQARLNPVAVRGEVS
                     GFTRASSGHCYFALKDDTGQLRCAMFRRAAGLLDFVPREGDAVELRGRLTVYEPRGDL
                     QLVVETMQRAGQGALFEQFLRLKARLQAEGLFDPARKRPLPALPRGIGLVTSRGAAAL
                     HDVLSTLARRVPHIPVLLVPAAVQGAGAPAELSQALQKLYLLAQSGQAPQANLARNAG
                     APPIDLILLVRGGGSIEDLWAFNDEPLARTIAASPVPVVSGVGHETDFTIADFVADLR
                     APTPTAAAELVGAPQAQWLAELQARAERLQRGLRHRLDAEAQRLDWLNAHLARPSQAV
                     AAERVRLAQLQQRLQHAQALRLERERARWQTLAARLPRSLTAPQAAEGARLQRLAERL
                     PAALARQLGSADERLQRAALRLSLLDPRLVLERGYAWLEDAQGRPVTRAAQTHDGQAL
                     AATLADGRVDLTVRGRRSG"
     gene            17486..18067
                     /locus_tag="PKN51_07310"
     CDS             17486..18067
                     /locus_tag="PKN51_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNT84944.1"
                     /translation="MEHTLPPLPYAIDALAPHYSQETLEYHHGKHHNAYVVNLNNLQK
                     GTEFESMTLEDIVRKSSGGIYNNAAQVWNHTFFWNCMKPNGGGEPSGALAAAMAAKWG
                     SYAAFKEAFVKSAVGNFGSGWTWLVKKADGSVDIVNTGAAGTPLTTADKALLTVDVWE
                     HAYYIDYRNLRPKFVETFLDKLVNWDFAARNFG"
     gene            18304..>19226
                     /locus_tag="PKN51_07315"
     CDS             18304..>19226
                     /locus_tag="PKN51_07315"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNT84945.1"
                     /translation="MSDKSPTIVYTLTDEAPLLATASLLPVVSAFAAQAGIQVDTSDI
                     SVAGRILGQFPDCLSEAQRVPDNLSELGKLTLKPEANIIKLPNISASVAQLKAAIAEL
                     QGKGYKIPDFPENPKDDKEKEIRARYNKCIGSAVNPVLREGNSDRRAPRAVKEFARKN
                     PHSMAEWSQASRSHVSHMHGGDFYHGEKSMTLDKARDVKMELLTKSGKTIVLKPKVSL
                     QDREVIDSMFMSKKALVEFYEKEIEDARKTGVMFSLHVKATMMKVSHPIVFGHCVKIF
                     YKDAFAKHQKLFDELGVNVNNGMVDLYNKIAT"
BASE COUNT         2928 a         6395 c         6840 g         2963 t
ORIGIN      
        1 tggtcggagc ggcgggattc gaactcgcga ccctctgctc ccaaagcaga tgcgctacca
       61 ggctgcgcta cgctccgaca aggggcgcat tctaccttgt ccgctcaccc catcggccgg
      121 cgtggacagg gcggtcgccc gtatggtcga aagctatatt tgtgatagct gccggcgctt
      181 gctggacggg cgcgagcggt cgattgcatt tttaggctca gctgccgcgc gtgaccggca
      241 tctccaccgc cttggcgccc ggcatgtgct tggccagctc cagcttggcg atggcgttgc
      301 ggtgcacctc gtcggggccg tcggccaggc gcagggtgcg ctggtgggcg tacatggaag
      361 cgagcggaaa gtcgttgctg acgccgccgc cgccgtgcgc ctggatggcc cagtcgatga
      421 ccttggtgga gacgttgggg gccaccacct tgatcatcgc gatctcggcc tgcgccacct
      481 tgttgcccac ggtgtccatc atgtaggcgg ccttgagcgt gagcaggcgc gcctggtcga
      541 tcatgcagcg cgactcggcg atgcgctcgc gccacacgcc ctgttcgctg atgcgccggc
      601 cgaaggccac gcggtggttc aggcgctggc acatcagctc cagcgcgcgc tcggcggcgc
      661 cgatggagcg catgcagtgg tggatgcggc cgggccccag gcgcccctgg gcgatggcaa
      721 agccccggcc ctcgccccac agcaggttgc cggcgggcac gcgcacgtcc ttcagctcga
      781 tttccatgtg gccgtgcggc gcgtcgtcca tgccgaacac cgtcagcggg cggatgacct
      841 tgacgcccgg cgtgttggcg ggcaccagga tcatggcctg ctgcgagtgg cgcggcgcgt
      901 cggggtcggt cttgcccatc acgacgtaga tcgcgcagcg ggggtcgccc gcgcccgagg
      961 accaccactt gcggccgttg atgacgtaat ggtcgccgtc gcgctggatg cggcattcga
     1021 tgttggtggc gtcactcgat gccacggccg gctcggtcat caggaaggcc gagcggatct
     1081 cgccgcgcag caggggttcc agccactggt ccttgttggc ctcggagccg tagcgctcga
     1141 tggtctccat gttgccggtg tcgggcgccg agcagttgaa gacttcgggc gcccaggaca
     1201 cgcggcccat gatctcgcac agcggcgcgt attcgaggtt ggacaggccc tctggcgcgc
     1261 gcttggactt gggcaggaac aggttccaca ggccttgctc acgtgccttg ggcttcagct
     1321 cctcgatgat gcgcgtggga atccaggcgt tgcccttggc gcggttggcc gccacttctt
     1381 catagaagcg cgcctcgttg gggtagatgt gctggtccat gaaggccaat agccgggcct
     1441 gcatgtcctg caccttgggg ctgtagtcga attccatgac gttctccttt taggcaatca
     1501 aatcaggcgc cagcccttgt gtggcgagcg ccgggagcta tgctttttga gcaaacgacc
     1561 aggccagctc ggccatcggc cgggcacccg cgccgctggc gcgcgcctgc gcgctgctgg
     1621 cggtgccggc ttccacccgc ttggcgatgc cctgcaggat ggcggcgatg cggaacatgt
     1681 tgtaggcgag gtagaagttc cagtcgggtg ccagctgctc gggcgtggcc aggccggtgc
     1741 gctcgcagta gcggtggatg tagtcctgct cggacgggat gcccaggctg gccagatcga
     1801 cgccgccgat gccgcggaac gtgcccggcg ggatgtgcca ggccatgcag tggtagctga
     1861 agtcggccag cgggtgaccc agggtggaca gttcccagtc cagcaccgcc aggatgcgcg
     1921 gctcgctggg gtggaagatg acgttgtcca gccggtagtc gccgtgcacg atggaggtca
     1981 ggccgtcgtc gcgcgcgctg gcgggcatgt gcgtgggcag ccattcgatc aggcggtcca
     2041 tttcggtgat gggctgggtg accgaggcct ggtactgctt gctccagcgg ccgatctggc
     2101 gctcgaagta gttgcccggc ttgccgtagc ccgccaggcc gcgcgcggcg tagtccacct
     2161 tgtgcagcgc ggcgatgacg cggttcatct cgtcgtagat ggcgccgcgc tcggcgggcg
     2221 tcatgccggg cagggtctgg tcccacagca cgcggccctc gacgtactcc atgatgtaga
     2281 aggcgcggcc gatgacggat tcgtcttcgc acagcgcgtg catgcgcgcc accggcacgg
     2341 aggtggaagc caggccgcgc atgacggcga actcgcgctc gatggcgtgc gccgacggca
     2401 gcagcttggc gacggggccg ggcttggcgc gcatcacgta ggtgcgcccg ggcgtgatga
     2461 gcttgtaggt ggggttggac tggccgccct tgaacatctc gacgcccagc gggccggcga
     2521 agtcctccac gtgctgcgtc atccaggcgc ccagggcggc ggtgtcgaag gcgtgcgccc
     2581 cggacaccgg ccgggtgccg acgaactgat cggtgtggct catgtcactg ctctccctcg
     2641 ctgatgcgca gcagcgcgtc gcggtcgcag accaccaggc ccgtcggctc gacgcggatc
     2701 acgccctcgc gctccatggt tttcagctcc tggttgacgc gctggcgcga ggcgcccagc
     2761 agttgcgcca gctcttcctg cgccagttgc agggcgatgc ggatttcgtc gttggacgac
     2821 aggctgggca cgccgtagct gcgcaccagg tggctgagct gcttggccag gcgcgcgcgc
     2881 agcggcaggg tgttgagatc ctcgacctgg ccgaacagca gccggatgcg ccgcgcctgc
     2941 agttgcagca gcgcctcgta cagctcggtg tgctgcgcca ggatcttgtg gaagtcggcg
     3001 cgcgccacgc acaggatggt ggtgacgcca tgcgcatagg catcgtgcgt gcgccggtcg
     3061 ccgtcgaaca tcgccacgtc gccgaaccac acacccggct ccacgtaggt cagcgtgatc
     3121 tgcttgccgg tgagcgtggt ggagctgacg cgcaccgcgc ccttggcgca ggcgatccac
     3181 tgctcggggg gttcgccacg ggcgctgatc agctcgccgt cggggtagcg tttgacaaag
     3241 gcgcatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg ccacgggcgc
     3361 tgatcagctc gccgtcgggg tagcgtttga caaaggcgca tcgaaggatg tcgtgccgca
     3421 gcgaagggga tagagaggaa aaccagcgac cactgttgat cgcctcgcgt tcctccatgg
     3481 taagaatggg ctcgtccatg gtctgtcttt tgggtgactg caagaggcgg cattgtcgcg
     3541 ccggggacgg ggcccgccct ggccgccggc ctgccgccgg gaactactcg tagcgcggcg
     3601 tctgcttggc caggaaggcc gcgatgccga tgccggcgtt ggggtggtgc aggttgcgca
     3661 cgaagtggtc gcgctccagc gccaggtggc tggacaggtc gttgcgatct gcttcgccga
     3721 ccaattcctt gatgctggcc atcacgttgg gggcgcgggc gcccaattgc ccggcccagt
     3781 cgagcgcact gcccagcgcc tggccggcgt cgcacagccg accgaccacg cccagctcgt
     3841 gcaggcgctg cgcgccgatg cgttcgcccc ccatcagcag ctggttgacc agctggcgcg
     3901 gcagcgcgcg cgccaggctc cagctgccac cgccgtcggg cgacaggccg acgctgctgt
     3961 aggccatgac gaacaccgcg ttgcgcgccg ccacgatcag gtcgcaggcc agcgccagcg
     4021 aaaaaccggc accggcggcc gggccttcga cggcggcgat gaccggcttg gggaaggtgc
     4081 ggatcgcttc gacccagtcg tgcagacgct cgatgctctg ggcctgcact tcgggcggcc
     4141 gctggcggtt ggcctgcagg cgctgcaggt tgccgccggc gcagaagacc tggccttcgc
     4201 cggtcagcac cacgcagcgc acctcggggt tcgactcggc ggcgctcagc gcctcgacgc
     4261 cggcggcgta catctcgggc ccgagcgcgt tgcgatgctc ggggttgctg atggtcagca
     4321 ccatggtgct gccttcgaag gtgctgcgca attcggcggt catgagggtg gtggagtggg
     4381 ctgaacggat tcaaacatcc ctacgcagag ggcgcgaaag tttcgcagag aacgcaaagg
     4441 agacaacttc catttgaggg ccgtggccga cttctgcgtc gctcactgcg gattccacgg
     4501 cctccaacat gcctggtgct gtctgctgac cctttgtctt cgcgcgcggc gcctcattgc
     4561 cgtggcgccg cagtccttta cccgcccccg gtggatgctc ttgcttgtgc gacttctgcg
     4621 cagtcttggc gtcctctgcg tacggatgtt tccagcattc aggcttcctc gtgcagcagc
     4681 gacagcccga tggcgccgcg gcggcgcagc caggggctgg ggcggtagcg cgggtcgccg
     4741 tagacggtct gcaggttgaa cagcacttcc agcatgttgg ccgggcccca gcggtcgccc
     4801 atggccagag gcccgagcgg gtagcccagg cccagggtga cggcggtttc cagatcctgc
     4861 gggctgcaca cgcgctgctg gcacatgtcg gcggcgatgt tgacgatgtg ggccaccacc
     4921 cgctgggtga cgaagccgcc cgaatcgtgg atcacgctca ccggcttgcc gtcgcgcgcg
     4981 aacagggcgt gggcggcgtc gcgcatgtcg ctgcgcgtgg cagggttggt ggccagcacg
     5041 cggcggcggg tggcggcgtc ttcgatcagc aggtcgatgc cgacggtgcg cgccggatcc
     5101 agccgctcga ccacggccac cgtggtgacg tcgaaaccca gtggcgccac cagcgtcagc
     5161 gcctgcggcg agggcgactg gccggtttcg atgcgggcgc ccaggtcctt cagcagctgg
     5221 tacagctccg cacggcgtgc ggcgcgggtc gatacccaca ccggcggcag ctccgccacc
     5281 tgcggcgcgg cgggctccgg cgccacctgc gcgctgccgc ccaggtagcg gtaaaaacct
     5341 tcgccactct tgcgacccag cacgccgccg gccagccgct gcgcggtgat gacgctggga
     5401 cggtagcgcg gttcgtcgaa atactggcgg tagatcgact ccatcaccgg gtgcgaaacg
     5461 tccagcgctg tcaggtcgaa cagctcaaac gggcccagac gaaagcccac ctggtcgcgc
     5521 aggatgcgat cgacggtgac gaaatcggtc acgccttcgc ccacgatgcg cagcgcctcg
     5581 gtgccgtagc cgcgcccggc gtggttgacg atgaaacccg gcgtgtcggc cgcctgcacc
     5641 ggcgtgtggc ccatctgacg cgcgtagtcg gtcaggcgct ggccggtggc ggcgtcggtc
     5701 ttgaggccgg cgatgacctc gaccaccttc atcagcggca ccgggttgaa gaagtggtag
     5761 ccggccagtc ggcccgggtg cttgagcccc gcggcaatcg ccgtcaccga cagcgaggag
     5821 gtgttggtgg ccagtatggc gtcgggccgc acgatgcctt ccagctcggc aaacagcgtc
     5881 ttcttgacgt ccaggcgctc gacgatggcc tcgatcacca ggtcgcagtc ggccagatcg
     5941 gccagcgtgg cggccggcgg cacgcgggag acatggctgt cggcctgctc acgcgtcaga
     6001 cggcctttgt ggaccagctt gtcccactgc gccgacagtg cttctcgggc cttggcaacg
     6061 gcgtcgggct gggtgtcgta caggcgcacg gtgctgccgg cctgggcggc gatctgggca
     6121 atgccgcgcc ccatggcgcc ggtgccgacg atgccgacgc gggaaaagac gggtgtgttc
     6181 atgacgacac attatggcgg cagcgcgcgc cgcgtcgcag cccacaaaaa ctatcaaaaa
     6241 aatagctgct ggcgctgacc aggcgggcgc cggagaccga tttgttgagt acctcctgcg
     6301 gtggctggcg aggcctcagc gccgcatctg cagggcgccc gggttgacga tctgcgtggg
     6361 tgtgccgcgc aggtagttga ccacgttgtc gaaggcggcg ccgaagtaca gctcgtagct
     6421 gtcctgctcc acgtagccga tgtgcggggt gcagatgcag ttctccagcc gcagcagggc
     6481 gtggccctgc aggatgggct cgctctcgaa cacgtccacc gccgccatgc cggggcggcc
     6541 acggttgagc gcgctgacca gtgcgtcgtg ctcgatcagc tcggcgcgcg aggtgttgac
     6601 cagcagggcg gtgggcttca tgcggctgag gtcttccagc cgcacgatgg cgtgcgtggc
     6661 ctcgaccagc cgcaggtgca gcgacagcac gtccgacagg gcgaagaact cggcccgcga
     6721 aggcgccacc tggtggccgt ccaggcgcgc gcgcgtcatc gaatccgacg atccccacac
     6781 cagcacctgc atgccgaagg cgcgcccgta gccggccacg atctggccga tgcgcccgta
     6841 gccccagatg cccagggttt tgccgcgcag cacctgcccg aggccgaagt tgggcggcat
     6901 ggacgccgac ttcagccccg actgctgcca ggcgccgtgc ttgaggttgc cgatgtactg
     6961 cggcagccgc cgcatggcgg ccatgatcag cgcccaggtc agctcggccg gcgccaccgg
     7021 cgagcccacg ccttcggcca cggcgatgcc gcgctcggtg caggcggcca cgtcgatgtg
     7081 gctgcccacg cggccggtct gcgaaatcat cttcagcctg gggagctttt cgatcagctg
     7141 gcgggtgatc tgcgtgcgct cgcggatcag caccagcacc tcggcatcgc gcagacgcac
     7201 cgacagctgg cccaggccct tgaccgtgtt ggtgtacacc ttggccgggt agggctccag
     7261 ccgggacgcg caggccagct tgcgcacggc gtcctggtag tcgtcgagga tcacgatgtt
     7321 catgcgcgca ttgtgcctct ggccagcggc cagctgcgca cgcgcatggg ggccgaccgc
     7381 ttctcgggcg gcgtccggac ggggcgaatt cgaggcccgc gtcaggcgct gaatacgaag
     7441 cgcgtgccgg tggaggagcg cacgaagcga tcgcgcattt ccgactgcgc catcgagatg
     7501 aaggtttcgc cggagcagtg catggggatc aggaagtcgg ggttgatggc cttcaattcg
     7561 gccagggttt gccgctgata ctccggcgtg tgcggggcaa ggtgaaaacc tcccagaatc
     7621 gcatggacct tgtcggtgcc ggccacctgc atggcgcggc gcaccgaatt gacgatgccg
     7681 cgatggccgc aggaggtcat gacgaccagg ccgcgccctt tcagctggta gacgatggcc
     7741 tgctcatgct cgaagtcgtc cggcaccgct gcggtcaggc tctgcttggc cggcgacagg
     7801 ccttccggcg cacaacccag cccagcttgg atgccaggcg tcatgcgtgt gggtgccagc
     7861 acacgttcga acgaactgct ggggatgacc ccggtggtga acccgtgcga ggccaggatc
     7921 gaggggcgct gggcgaacgt catggtcatg cccgactgac gaatggcttc gcggtccagc
     7981 gcgccaaaac tccctgcgtc acccggcacg ttcagctcgc gtgaacagaa gcactcttca
     8041 cttccgagat agaagggaag cccaagcttg agtttgctct tgtgagcgtc gagaaagccc
     8101 accatgccac cgaaatgatc gtagtgaccg tgactcaggg cgatggcgtc gagtttggac
     8161 acatcgatgc cgcacagcag caggttgttg agcaaggctt cgggggtgta ggcaaagtcg
     8221 agcagtacct gacgtacttc gtcgccccgc cctgattcga tgtgcagcga caggccccac
     8281 tcgttctgca gcgtacgacg gggcggccga ccaggtttga catcgaagcc cagtcgctgc
     8341 acctgcaggt tgcccagcgt gcgcggtggc tcgaaggcat ggtggtacga gtcggtcacc
     8401 actcggatcg ccacgcgatc gaccacggga acgtccgcag gcttgtcgat ggttgcagcc
     8461 accgccacac ggccaccgaa caaaccgcct gcggccagca aggacagcga gcctgcgccc
     8521 ctcagcagtt gccgccgtcc ggcgctggcg aggtagctgt cgtcggtggt ggtttcaatg
     8581 ggcatggctg gcttcctccg ggttgattgc ggcgattcat gctatcggct tcagggtgcg
     8641 ttgtcacgga ttgccgcaaa gagggcgccg cagaacccgc cgccaggccc atttgtcgac
     8701 acgacaccac gcgccccgtc ggggcacgat tcatgcgcat gcttcatgcg cagcgcggtt
     8761 gtggcgggct gttcgccagt tcggccgcgg ccagccgctc cagctcggcg cagacgtcga
     8821 tcaggcggcc cgaaatgcgc aggcgcgccg gcgcgtccgg ggcctgggga tcgcgcaggc
     8881 ggagggtgcg tacgggtggc gcgctgcagg gggcggcggt cggggcggga tccgggtgga
     8941 gggggagacc gccggcgcca ggttgcagcg cgcgctgccg ggccgggcct gaccaggcgt
     9001 tgtcgggggc gggcctgacc agcgagctgc cggcgctgcc cgggcggcgc gggcgccggt
     9061 agctgggcac ccggtagtcg tccaggcaca ggacggtggc cgcggtgggc tggtgggtgg
     9121 tgaatcgggc catgtgaaac ctttcagagt gaaacaggtt ggacacaggc gagattgcgg
     9181 tgaagaggcg ccgccccagg gtgtggatgg cccggccatc tgcacgcccg ccacctgtgg
     9241 ggcgccgtga ctgccgcgct acatcagcat gctgttgcgg atcaggccga cggccaggcc
     9301 ctcgatttcg aacggctcgc ccggctgcac cacgatggtg gggtagtcgg ggttttcggc
     9361 atgcagctcg atctggtgct ggtggcgatg aaagcgcttg accgtgacgt cgtcacccag
     9421 gcgcgccacc acgatctggc cgttgcgtgc ttcgcgtgtg gcgtgcactg ccagcaggtc
     9481 gccgtcgagg atgccggcgc cctgcatcga catgccgcgc acccgcaaca ggtagtcggg
     9541 gcgctgggcg aacagggtgg gttcgaggtg gtaggtttgc tccacgtgct cctgcgccag
     9601 gatgggcgag ccggcggcca cgcgaccgac cagcggcaac gccagttgcg ccatgccggg
     9661 cagcgtcagg ctgaaggcgt cgcccgccag gcggctgcgg gccgcgcctt tcaggcggat
     9721 gccgcgcgaa gtgccgctga cgagttcgat cacccctttg cgtgccagag cctgcaggtg
     9781 ttcttcagcc gcgttggcgg acttgaagcc cagctcggcc gcgatttctg cgcgcgtggg
     9841 cggcgccccg gtgcgcgcga tggcgtgttg caccagttgc aggatctgct gctggcgggc
     9901 agtgagcttg ggcatgtcgg acatggtggc tgacctcagg tgtctggaat ctggatcgat
     9961 ccacagtgac tgtgatttta cacagttact gcgcgatgac aagcggtggt tgatcgcccc
    10021 acggccagcc ggatatgtta catatgtgta tttaagtatt gcttatgatt gaattcgaat
    10081 ggccatcgag cccgggccac aacgttgcaa tcgaggagac acatcgtcat gcaagccacc
    10141 cccatctccc cgttcggtcg cactgcgcgt gtggccgtgt tgagcgcact gtgcgccgct
    10201 gtggccgcat gtggcggcgg aggtgacgcg gaccccgcac ccgcacccgc acccgcaccc
    10261 gcacccgcac ccgcgcctgc gcctgcgcct gcgcctgcgc cagcaccagc accatcaccg
    10321 gtcagtgcgt gcggcgacat gaacgagttg ctgagcgcca tcaatgccgt gcgcgccacg
    10381 gcgcgcagct gcggtgggac ggccatgccg gctgcggcgg cgctgagctg gaacgcgcta
    10441 ttggcgcatg ccgccgcggc gcactcgacc gacatggcga cccacaattt cttcagtcac
    10501 acgggctcga acggcagcaa catggcgcag cgggtcgacg cggctggcta tgtctggcaa
    10561 gccctggcgg aaaacattgc ggctggcaac gcgagcgtcg cggccaccgt cagccaatgg
    10621 gtgaacagcc ccggccactg tcaggccatg atgagcgcca cctacgtgca tgtgggcggc
    10681 gcctgccgct acaacgccag cacccagtac ggctactact ggacgatcga tctgggcaag
    10741 ccgttctgag cctcagagcg gtgacgccgc acgcccagga cgcggcaaaa aaaatgcggc
    10801 gccagggcgg gcgccgcttc ggtgagggca ggcggtggct cagcggacgc ggatggggct
    10861 gcccccagga acgccaactc ggcgcatcag ctggccagcg ccgccaaggc gcgggcggtg
    10921 atttcttcca ccgtgccggt gccgctgatc tggcggtact tgggcgcggc ggcgggctcg
    10981 cgccgggccc agtcgccgta gtagtcgacc agcgggcgcg tctgctggct gtagacgtcc
    11041 aggcgcttgc gcacggtttc ttccttgtcg tcgtcgcgct gcaccaacgg ctcgccggtg
    11101 acgtcgtcct tgccctcgac cttgggcggg ttgaacttga cgtggtaggt gcggcccgag
    11161 gccgggtggc tgcggcggcc gctcatgcgc tcgatgatgg cgtcgaacgg cacgtcgatc
    11221 tccagcacgt agtccagctt gacgccggcg gctttcatgg cctcggcctg cgggatggtg
    11281 cgcggaaagc cgtcgaacag aaagccgctc gcgcaatcgg gctgggcgat gcgctccttg
    11341 accaggttga tgatgagttc gtcactgacc aggccgcccg actccatcac gcccttggcc
    11401 tgcaggccca gcggtgtgcc ggccttgacc gcggcgcgca gcatgtcgcc ggtggagatc
    11461 tgcgggatgc cgtacttctg gcagatgaag gccgcttgcg tgcccttgcc ggcgccgggg
    11521 gcgcccaaca ggatcagtct catcgtgctc ctctgtgtgt ccgtgggagg ggaaagaccg
    11581 gcgcgcatgg ccacaagggg ccgggccgga acgggttgcg ttcgagaata gcacgcgggg
    11641 ccttgtggcc ggcttacaga caaggcgtgc cggccttggg ctcgcctgat acacgaactt
    11701 gctatcaacc tggtccaaca tgattcatga cgctcgcccc cggctgcgct tcgtcatgtc
    11761 ggcgcagccg gggtcacagg ttctcttctt gaacgccaag ccgtaggctg ggtgcccggc
    11821 cgtgctcggg gttgtcctgc cacatccggc ctcatgcctt gtctggcaag cgtcggcagc
    11881 tatggtttga atagcttagg gagcgggcgc gctcagcccg caccgctcgc cagcagggcc
    11941 cgcacgcgcg ccaggtcctc gggcgtgtcg acgccggggc cgggcgcctg ggccgtcaca
    12001 tgcacggcga tgcggtggcc gtgccagagc gcacgcagct gctccagcgc ctcgcattgc
    12061 tccagcggcg ccggcgccag gctggcgaag gcccgcagaa agccggcgcg gtagctgtac
    12121 aggccgatgt ggcgcagcgg cgccggctgc gccggcaagg ccgtgacgcc gccggcaaag
    12181 ccgtcgcgcc accagggcag cggcgcgcgc gagaacgtga gcgccagccc ctgggcgtcg
    12241 gtcaccacct tgacgacgtt cgggttcagg aaatcagcta cgttttcgat agcgtgtgcc
    12301 gcagtcccca tgcgagcgac aggcgaactg ctcaacaaag cggcgacggc atcgatgagc
    12361 gccgggtcga tcagcggctc gtcgccctgt acgttgacca ccacgtcgtc gccgtccaga
    12421 cccagcagtg cgcaggcttc ggccaggcgg tcgctgccac tggcgtgatc ggcgcgcgtg
    12481 gcgatggcgc gcacgccgtg ggcggcgcag gcgtgcagga tctcgggcgc atcggccgcc
    12541 accaccacct gcgccgcgcc cgagcgctcg gcgcgctcgg ccacgcgcac gatcatcggc
    12601 ttgccagcga tgtcggccag tggcttgttc ggcagccggg tcgaggccag ccgggcgggg
    12661 atgaggacgg tgaagctcat ccgcccacca gcggcgtgcg cggtggcagc gcggccagtt
    12721 cgtcgtcgct gagggcgcgc gcctcggctt ccagcatgat ggggatgcca tcgcggatcg
    12781 ggtaggccag gcgggccgag cgtgagatca gctcgccctt gtcgcggtcg tggtccagcg
    12841 ggcctttggt gacggggcag accagcagtt cgagcagctt ggggtccatg gtgatccgat
    12901 cagtgggcag aggagggaga cagcaggttc atgaccagca ccccggccac gaccagcgcc
    12961 atgcccagca gcgcccagcc gtccagccgc tggccataca gccaccagcc ggccagggcg
    13021 atcagcacca tgcccaggcc cgaccaggtg gcgtaggcga tgccgaccgg gatggtgcgc
    13081 agcgtcagcg acagcagcca gaacgccagc gcatagccga tcaccaccag cgccgtcggc
    13141 ccgccgcgcg acaggccgtc gctggccttg agcgcgctgg tggcgacgac ttcggcgacg
    13201 atggcgccca gcaggaacag gtggtttttc atggcgcggc ggtgggccgg tcggcccggc
    13261 tggcgggatg atagccgcgc gcggccagcc gctcgtcgag cgcggcgaag aacgctgccg
    13321 gcacctgcag gcgcagcggc acggccagcg cgtcggggcg gtggcgccac agtttgaccg
    13381 cgtctttctc ggtgcaaatc aggttctggc gcccgtccag caagcgcaag cagctatcaa
    13441 aatcgtagtg atcgggcaga gccagggtgt cggtcagggt caggccggcg gcgcgcagca
    13501 tggcaaagaa ggcctcgggc cgtgcgatgc cggccagcgc ggtcagcggc tggccgcgca
    13561 gatcggccag cggctgccta ccgccatcgg cacgcagggc gtgctcggcc agcgcgcggg
    13621 tggcgccgaa ggcggccggc gcgggggcct ggtcgggggc gggcggctcg gtccacagca
    13681 ccaactcggc cgggcgcggc ccgggttcgc gcggatcgac agggctgccc ggcccgggct
    13741 cgcgcgcagg gctgcgcggc cacggctcgc gcaggggccc ggccggcagc agccagtggt
    13801 tgccgcagcc gcgggcgtcg aacaccacca cctccaggtc gcgcgccagg gccaggtgct
    13861 gcagcccgtc gtcgcagacc aggacgtcgg tgtccgggtg cgccgccagc agggcgcggc
    13921 cggcctgggc gcgctggcgc gccacgaaca ccggcacgcc ggcgcgccgg cgcagcagca
    13981 gcggctcgtc gccgacctcg cgcgggtcgc tgtcgggctg cacggcgcgg cagtcgtcgc
    14041 tggcgcggcc gtggccgcgc gagacgatgc cgggccgcca gccgcgcgcc tgcaggtgcg
    14101 ccaccagggc cagcgtggtc ggggtcttgc cggcgccgcc gacgatcacg ttgccgacca
    14161 cgatcaccgg cgccggcagc cgcgccacgg gcagggcgcc gctcaggtag cgccggcgcc
    14221 ggtgcgccgc cagcgccccg tacagccgcg acagcggcca cagcaggcgc gccagcaccc
    14281 cgcgccgctg ccaggcggcg cgcaggcggc gttcgatgaa ggcgcgcagc atcgggtcag
    14341 cggttgacca cgggccggcc aggcccgcag ggcgcgccgc cacgcacccg cgcgaggcgc
    14401 cgcacgctgc catggcaagg ccgggcgacg cagagcggcg atgttcgggt gtgcaaagcg
    14461 ggcaagggtg gccttctttc aagctcgcgg cgagcggcgg ctgtgcgcag cgcgcggcgg
    14521 caggcgcagc atagcgccgg caggggccgt cacctgcccc cgctctgggc ggcaaaggtg
    14581 atctgcgtca ggccctcgcg gcgcgccgcg tccatcaccg tcaccaccgc ctgcaccggg
    14641 gcggtggcgt cggcgctgat gatgagcacg ctgtccttgc cggccagggc gcgcagggca
    14701 cgcgccacgg cgtcgacgct gcgggcttcc agcggctggc ggttgacggc gtagcggccg
    14761 tcggcggcga ccgccaccac gatttccttg ggccgttcgc gcgaggcgtc ggcgtcggcc
    14821 accggcagcg tcacctgcag ttcggtgaac ttgctgtagg tggtggtgag catcagaaag
    14881 atcaggatca ccagcagcac gtcgatgaac gggatcaggt tgacctccgg ttcctcgggc
    14941 gggtgcgggc ggaacttcat cgcccggctc cggcatcggc ggcgcggccg gggcacagcg
    15001 gctccaggtg gcgcgcaaag cgctcggcgg ccagctccag ctccagcacg tagccgtcca
    15061 cgcgggcgcg gaagtagcgc cacaggatca gcgccgggat cgccacgatc aggccgaacg
    15121 cggtgttgta cagcgccacc gagatgccgt gcgccagctg cgccgggttg ccaccggaca
    15181 gcgaaccggc cggcgcctgc gagccgaaga tctcgatcat gccgatcacc gtgcccagca
    15241 gacccagcag cggcgccgcc gaggcgatgg tggccagcgc cggcaggtag cgctccagcc
    15301 ggtgcgccac ctggcggccg gcggcttcca tggcggcgcg catttcttcc gggctgcaac
    15361 ggggattggc gttgatggcg cgccagccgg ccgccagcac ctcgcccagg gcgctgtgca
    15421 gctccagctg gcgcaccatg tcgggcggcg gcacgctttc gcgcgagacg ccgatggctt
    15481 cgtccaccag ctgggcaccc agcacgcggc ggctctgcaa ctggacgaag cgctcgatgg
    15541 cgaaggccag cgccaggatc gaacacagga tgagcggcca gatcggccag ccggcggctt
    15601 gtatgatcga cagcaagggg tgctttctcg ggcgcaggcg cggcccgcag tgtcggaaaa
    15661 aagagtgcag gcgattatgg cgcagcccgt gtgccccaag cgccagccgc gcgaccaggc
    15721 cccggcagcg tggtgaaccg acggcacgtc cgggtaacta ccgatgttgt ggataacttt
    15781 gtggaatatt cgcccggtct ggcccgttcc agctgcggcc gccacgattg tgaccgattg
    15841 tgcgtcgcag catcaagaaa attgtgtttg aaatcaatgg cttgcaccga tctctcgtgg
    15901 ttttcggcgg gtggcccgag gctgatgggg gcgggcgccg cgctgtggag tactggcgcg
    15961 gcctgggccc cggaatcggc cgccagagcg catgaatact gggtttgaac cggcagcgcc
    16021 agcattggcg ccgcgcgtct gggcggttgg ggcgctggcg cgagccgtgg cggacctgtt
    16081 gcaggcgcgc ttgaacccgg tggcggtgcg cggcgaggtg tcgggcttca cgcgcgcttc
    16141 cagcgggcac tgttattttg cgctcaagga cgacaccggc cagctgcgct gcgccatgtt
    16201 ccgccgcgcc gccgggctgc tggacttcgt gccgcgcgag ggtgacgcgg tcgagctgcg
    16261 cgggcgcctg acggtgtacg agccgcgcgg cgacctgcaa ctggtggtgg aaaccatgca
    16321 gcgcgccggc cagggcgcgc tgttcgagca gttcctgcgg ctgaaggcgc ggctgcaggc
    16381 cgagggtctg ttcgaccccg cccgcaagcg cccgctgccg gcgctgccgc gcggcatcgg
    16441 cctggtgacg tcgcgcggcg ccgccgccct gcacgacgtg ctcagcaccc tggcgcgccg
    16501 cgtgccgcac atcccggtgt tgctggtgcc ggcggcggtg cagggcgccg gggcgccggc
    16561 cgagctgtcc caggcgctac aaaaactgta tctgctggcg cagtctggac aagcgccgca
    16621 ggccaatctg gcacgcaacg cgggggcgcc gccgatcgac ctcatcctgc tggtgcgcgg
    16681 cggcggctcg atcgaggacc tgtgggcttt caacgacgag ccgctggcgc gcaccatcgc
    16741 cgccagcccg gtgccggtgg tcagcggcgt cggccatgaa accgacttca ccatcgccga
    16801 cttcgtggcc gacctgcggg cgcccacgcc cacggcggcg gccgaactgg tgggcgcgcc
    16861 gcaggcgcag tggctggccg agctgcaggc gcgggccgaa cgcctgcagc gcgggctgcg
    16921 tcaccggctc gacgccgagg cgcagcgcct ggactggctg aacgcccacc tggcgcgccc
    16981 gtcgcaggcg gtggcggccg agcgggtgcg actggcgcaa ctgcagcagc ggctgcagca
    17041 cgcccaggcg ctgcgcctgg agcgcgagcg ggcccgctgg cagacgctgg cggcgcgcct
    17101 gccgcgcagc ctgacggcac cgcaggcggc cgagggcgcg cgcctgcagc gcctggccga
    17161 acgcctgccc gccgcgctgg cgcgccaact gggcagcgcc gacgagcgcc tgcagcgcgc
    17221 cgccctgcgg ctgtcgctgc tcgatccacg gctggtgctg gagcgcggct acgcctggct
    17281 ggaggacgcc cagggccggc ccgtcacccg cgccgcccag acgcacgacg gccaggcgct
    17341 ggccgccacc ctggcggacg gcagggtcga tctgacggtg cgcgggcgcc gatcgggctg
    17401 acttcctaca atcgaccgtt gccgcgcgcc ggcccctggc ccaggcgccc atccattgcc
    17461 acccacgaac tcaaggagaa cccacatgga acacaccctg ccgccgctgc cctacgccat
    17521 cgacgccctg gccccgcact acagccagga aacgctggag taccaccacg gcaagcacca
    17581 caacgcctac gtggtgaacc tgaacaacct gcaaaagggc accgagttcg agagcatgac
    17641 gctggaagac atcgtcagga agtccagcgg cggcatctac aacaacgccg cccaggtctg
    17701 gaaccacacc ttcttctgga actgcatgaa gcccaacggc ggcggcgagc cgagcggcgc
    17761 gttggccgcg gccatggccg ccaagtgggg cagttacgcc gccttcaagg aggccttcgt
    17821 caaatccgcc gtgggcaact tcggctccgg ctggacctgg ctggtcaaga aggccgacgg
    17881 cagcgtggac atcgtcaaca ccggcgccgc cggcacgccc ctgaccacgg ccgacaaggc
    17941 cctgctcacg gtggacgtgt gggagcacgc ctactacatc gactaccgca acctgcgccc
    18001 caagttcgtc gagaccttcc tcgacaagct ggtcaactgg gacttcgcgg cccgcaactt
    18061 cggctgatcg cggcaccgag ccggcgcggt acgcaccggc gccacaccca ggccaccacc
    18121 ccggtggcct tttgtttttt tgacttcggc cagcaggcgc actgtggcca tttgccagaa
    18181 tacgccccgc aaccggcagc ggtgctctgt gagcgccgtg tcaggaaggc atgcgaccat
    18241 gccgcgcgca cgctacccgc gtgacggcgc ggcatgccga caacgaccat cggagatacc
    18301 ctcatgagcg acaagtcccc caccatcgtc tacaccctga ccgacgaggc gccgctgctg
    18361 gcgaccgcgt cgctgctgcc tgtcgtgagc gcgtttgcgg cgcaggccgg cattcaggtc
    18421 gacaccagcg acatctcggt ggccgggcgc atcctggggc agtttccgga ctgcctgagc
    18481 gaggcccagc gcgtgcccga caacctgagc gagctgggca agctgacgct caagcccgag
    18541 gccaacatca tcaagctgcc caacatcagc gcctcggtgg cgcagctcaa ggcggccatc
    18601 gccgagctgc agggcaaggg ctacaagatc cccgactttc cggagaaccc caaggacgac
    18661 aaggaaaagg agatccgggc gcgctacaac aagtgcatcg gcagcgccgt caacccggtg
    18721 ctgcgcgaag gcaattccga ccgccgcgcg ccgcgcgccg tcaaggaatt tgcgcgcaag
    18781 aacccgcaca gcatggccga gtggagccag gcctcgcgct cgcacgtctc gcacatgcac
    18841 ggcggtgact tctaccacgg cgaaaagtcc atgacgctgg acaaggcgcg cgacgtgaag
    18901 atggagctgc tcaccaagtc cggcaagacc atcgtcctga agcccaaggt cagcctgcag
    18961 gaccgcgagg tgatcgactc catgttcatg agcaagaagg ccctggtgga attctatgaa
    19021 aaggagatcg aggacgcgcg caagaccggc gtgatgttct cgttgcacgt caaggccacc
    19081 atgatgaagg tcagccaccc catcgtcttt ggccactgcg tgaagatctt ctacaaggat
    19141 gcgtttgcca agcaccagaa gctgttcgac gagctgggcg tgaacgtcaa caacggcatg
    19201 gtcgacctgt acaacaagat cgccac
//
LOCUS       DAOQTZ010000047        19207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3315_length_19207_cov_14.292607, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000047 DAOQTZ010000000
VERSION     DAOQTZ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 19207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19207
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3315_length_19207_cov_14.292607"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..983)
                     /locus_tag="PKN51_07320"
     CDS             complement(84..983)
                     /locus_tag="PKN51_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT84946.1"
                     /translation="MDKFQEMRVFVAVVDAGSFVRAADALDLSKAAVSRLVSDLEARL
                     GVRLLHRTTRKLSLTEVGEVFHERCRQLLDSVAEAEAELSTHSGAVIGRLKINVPFTF
                     GLLHLASLWPRFMAQHPQVVLDVTLSDRFVDLVDEGYDLAVRIARLQASSLVSRRLAS
                     THMVLCASPQYLREHGAPVHPAELEQHALISYTLLSTGEQWELQGPEGSLNVRISPRM
                     RTNSGDTCCAAAIGHQGIVFQPTFLVAQHLASGALVPVLPQYRSPEIGIYAVYPTRKH
                     LTPKVRALIDFLAEALRTPAWSG"
     gene            1104..1736
                     /locus_tag="PKN51_07325"
     CDS             1104..1736
                     /locus_tag="PKN51_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNT84947.1"
                     /translation="MKVTVIGAGHMGSAFVKQLVQAGHEVSVTARSRAKAERLAAAHP
                     GAKAVPAATAAAGADVIVLATGYGDAVEALKSVGDLQGKVVIDITNPLTADYMGLTLG
                     HSTSAAEEIAKAVPGAQIVKGFNTVFAQVLAEGADFGAGRSVTVFVASDSERARQTAS
                     ALAHSMGFEVVDAGGLKNARYLEPLAGLNIYLGYGAGLGTAIAPTWIRKA"
     gene            1760..2206
                     /locus_tag="PKN51_07330"
     CDS             1760..2206
                     /locus_tag="PKN51_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNT84948.1"
                     /translation="MSESLIQRLLATGNGSAALVLRVPVGIIFAAHGAQKLFGWFGGH
                     GLEGTGQFFASIGLAPGYLMALLAGAAEFFGGLALIVGLLVRPAGAALAFAMLVAIVS
                     AHGGKGFFLASGGLEYALALLAAALSLAFSGAGRCSLDRAFARSRS"
     gene            complement(2265..2615)
                     /gene="cutA"
                     /locus_tag="PKN51_07335"
     CDS             complement(2265..2615)
                     /gene="cutA"
                     /locus_tag="PKN51_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNT84949.1"
                     /translation="MAAPLPWLVVTTVGSREQAQALARTLVHLRLAACVQIEAIDSIY
                     RWQGEVLEDGEFRLLCKTQPAQYAQLEAAIRQHHPYQLPAIHAIATVAADPAYAAWVA
                     DNAGGPAAPTSGRR"
     gene            complement(2630..3790)
                     /locus_tag="PKN51_07340"
     CDS             complement(2630..3790)
                     /locus_tag="PKN51_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT84950.1"
                     /translation="MPGLLPHIDPQGLLEFSVVYTDRALNHMSVRFQGVMKDIAATLK
                     QVYHADAAVIVPGSGTFGMEAVARQFATGQKVLIVRNGFFSYRWSQILEMGQITAPEH
                     IRVFKARRTADGPQAPWAPAPIDEVTAAIAHERPAVVFAPHVETASGMMLPDDYVRRL
                     GEATRAVGGLFVLDCVASGAMWVDMQALGVDVLISAPQKGWSSAPACAMVCLSERALQ
                     VMESRTSTSYSCDLKKWHQIMRAYETGAHAYHATLPTMALLELRDAMAETRARGFAQV
                     REQQLELGHKVRALLAARGFPSVAAPGFEAPGVVVSYTRDPDIQNGRKFIEQGLQSAS
                     GVPLMCDEPADFSTFRLGLFGLDKWANVDRSVGQLATALDRMGHPAPAGAPA"
     gene            3966..4652
                     /locus_tag="PKN51_07345"
     CDS             3966..4652
                     /locus_tag="PKN51_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNT84951.1"
                     /translation="MDIPAFALAFGKSLLFALAAVLPILNPPASAPVFVNLTRGLDHD
                     TRAMLARRIGRHVVMLLAGAMLVGSYVLDFFGISLPIVRVAGGLIVASTAWRMLHVQH
                     LAQDDKAQLAQSLTADNVRIQAFYPMTFPLTCGPGSIAAAITVGASLHSRRVTEALVN
                     FAGGLAATLLVGLLVTLTFRYAGRLLRPLGEVGQIVFLRLMAFMLLCVGVQIVWDGAS
                     ALLQGLGNSK"
     gene            complement(4714..5526)
                     /locus_tag="PKN51_07350"
     CDS             complement(4714..5526)
                     /locus_tag="PKN51_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84952.1"
                     /translation="MLPWSAAAAPAARRLTGMVWQPSRQTLQPRGNWAQLGIRRLLVQ
                     WTAVDNLSFLPHAGLALQPPPLPDWDRIAGEPWAQEVIVGLAGLHAEPLARAALPALV
                     AQSRALCVAAAPWPLRIGGWYFPVEIDPGWQPPADLPERLNALPRPLWVSVYDSANLG
                     PHALLAWIERWLPTDVGVLFQDGVGVHAREPALARQYLIQLGARLGRTRVQVIAEAFR
                     PAPGGGFRSATAQEFLPQLDAYQDWPILAFDGPHYLNEALIQDLVAAGVRPA"
     gene            complement(5690..7384)
                     /locus_tag="PKN51_07355"
     CDS             complement(5690..7384)
                     /locus_tag="PKN51_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HNT84953.1"
                     /translation="MKTLRYFVALLSALCMTAATAADTGFRISIRLPWLIAPSAPPPV
                     APQAGPRTLVLYDAPPGQTFTKLGMAYAIMMRNLLGHFDSQVDMVPVHDYVTGQVNNY
                     DATFYLGAYYDNQVPPAFLADVTTTTKTVVWFKYNLWQIAWNPAYDFQNRFGFGFSGL
                     RGLNALPSAANPAPGFFDTITYKSLPFVKYYDYAGGAVAADPDVGIVAVTDTQKATTL
                     VPMSNPKTSETAPYIVRAGNFWYVADLPFSYIGPRDRYLVLTDLLHDMLGVNHATSHK
                     AMVRLEDVGAMVSVSAMKSLTNYLYQRRIPFSIATIPYYADPLGTYNGGVPMFVPMSQ
                     ASNLKTSLNYALARGGEIVMHGYTHQYSNMRNKYTGVSGDDYEFWNIVDNTPVMEDSL
                     AWALSRYQAGLQELSSNGYTATAWETPHYHGSALASKAAAQTFAKTYQRVVYFTADQP
                     NFNAPVAKDFGVGQIFPYPIKSDYYGQRVLPESLGNIEYDISSIDPTSNYNYTWQDIV
                     TNAQYVKTVRDGYASFFFHPFWLEPALGVPGLADFKKVIQGISNLGFTWTVPSQIN"
     gene            complement(7424..7711)
                     /locus_tag="PKN51_07360"
     CDS             complement(7424..7711)
                     /locus_tag="PKN51_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84954.1"
                     /translation="MKTLSTHPLRAGVSATLLLCSLAASATTVAPPPLPMGSAMAKPQ
                     VGASPTEEKRAEKAHKQPSVLRRNHLHDDSLAPPRQGAIPRATVSVPRDSK"
     gene            complement(7779..10652)
                     /locus_tag="PKN51_07365"
     CDS             complement(7779..10652)
                     /locus_tag="PKN51_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT84955.1"
                     /translation="MKRLSPSLALTLLLAALPALGQPVDLGPGVSGYTRFLVYPHLQK
                     GFQALREAQQTLAYEEFEQALKLAPDSAVIAGYLVEAYRHFGDHDKARQLLQRQLARH
                     PDDPRLRQALASLGPAAPTRPPAPQVTAGGAGDRAAELAAAPAPAPAPAPAVEAAPPR
                     ARAAATPDAASADARQTSRRDRASSAGGRPPSRSAPATTRLAALAPPAMPPANQAPQH
                     GARAAYALADAAYKASATGDFAAAARDARQALQLQPDNADYRRLLAYALLETGAYADV
                     EALAGERSSSDPAMVALADQARRQRAYVEFEAASRSLAAGDVDGAVTQAARGAQLAPA
                     TLAQQVQWLGALAAAQRWSALDDAASQVLQRPGMDDADIRLLRAHARQRLGRLDDANA
                     DYDRALALSSDPDTRSRNARLIVADAALAAGQTVRAEQLLAALSGPADAAVDARLRQA
                     SAGARRTLLPSPTVVPALATPRVVCVGSAHTPYCELWPGDAPADPGAVHADAALRAYA
                     ARSYAVAATQARSAVEQSPANRRYQLLLVRALLAADQPQQALERANEFLSHGEPEPEL
                     LALRSALHRQLEHPQAAAADARAALADPRLSVSSQIDLLLPSDPAQARAVFAAAQHSP
                     MMGELSDTDAAYLAVRVGDDASAAQAFDRAAQHGQLPDHALLDAGYVAGRLGRSQVAV
                     DYFKRAIDAAEAGRLALTPQRLFETRREVADRTRTWGAILSLGYRGVSPGGQMAGTPT
                     TLGDSLQAGMELYWRPAGYRDGSYYELYGGGFQTPWAKGAAPSGGPTTQAMLGARVKP
                     LSSLNLVLAIERRLKVGRLSSNDWLLRAGYSYTHGTDLRIDADSWFTVQVYAEAGRFL
                     RAHRSYATFEANLGRSIRLNDIDAQLVLFPHLVLAADHDSKLAPGQRSASGAGIGVAA
                     RCWFNQDRYQAPRSYLDVSLQYRARLGGDARGKGVFLRATLNF"
     gene            complement(10653..12869)
                     /locus_tag="PKN51_07370"
     CDS             complement(10653..12869)
                     /locus_tag="PKN51_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HNT84956.1"
                     /translation="MWWVDYVSSYLYGLKSVALALAVIILVSSLDDLFIDLAYWCRRL
                     WRSATIYRRHARARPADLQARDEKPMAIMVPAWQEHGVIDRMAELAATTLDYENYHIF
                     VGTYPNDPQTQSDVEKVCARFPNVHKVVGARDGPTSKADCLNNVLDAIFQFERRAGIH
                     FEGFILHDSEDVLSPMELRVFNHLVGRKDLIQLPVYPLPRPWTDFTSCHYLDEFAESH
                     GKDLLVREALVGQVPSAGVGTCFSRKAVMALLSHGKGIAFDVKSLTEDYDIGLRLKGM
                     GMQEVFVRFPMKPHHARDAGAPLGQNAREASTVCVREYFPSVTRAAIRQKSRWIIGII
                     YQGTKTMGWSRHWGLNYFLWRDRKGVITNFAGMLATLVLLQLVALWLYQRLVTDSYQF
                     LSIFEDSAWLTLLLVANLVLLCNRAVQRIYFVSSYYGIRQGLLSLPRMAWGNVINFFA
                     NWRALMQTLQAQDVRRIAWAKTEHDFPTLGDEGGAREPLGQILVAQSALTQEQLDEAL
                     AEPVPGLRLGAMLVHRGLVTPEQLAQAVATQSRADWEPFDSRQLGPELVACVPAAVAL
                     HYAVLPMRQTEDTLVLACESYLDPVSLAAIARKLDRKVSYVIVPKGEVTVGLRRWYGR
                     DGDLVAADAGRGGVHATPQTAALMWRVYVGGQLLLGDILCALGHVDPAALKSVLIQRA
                     KTGQRLGEFMVARGLLTPQVLDEALALQRSLQPSIDTLLGGLVPGRVGSGPLATEA"
     gene            complement(12856..14037)
                     /gene="wecB"
                     /locus_tag="PKN51_07375"
     CDS             complement(12856..14037)
                     /gene="wecB"
                     /locus_tag="PKN51_07375"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNT84957.1"
                     /translation="MDARVRDRNRGGVAAAASILIVCGTRPEIIKLAPLYHELRRQPW
                     AHVVWLHTGQHAQMAQPILDSFDIRPDLTLTRQGDSLLDFSLGCRAQLEAVLAGGRWA
                     AVVVQGDTESAFQGALAGFYHGVPVAHVEAGLRTYNLNRPFPEEGLRQMIGRIARLHF
                     APTARSRQALLSEGVPQQRIWVTGNTVIDAQQWVCAHRGIGRRVNGRGHLLVTMHRRE
                     NWGQDIERVCRAIARIAREQPDLEVLFPVHLNPVVHGPVHQLLAGLPNVRLTAPLDYL
                     EMQQALRDAWIVLTDSGGLQEEAPTFGIPLLVLREETERPEALEAGCAAIVGTATDTI
                     VEQVRRLWREPAEFLRMQQAGNPFGDGTASRQIARHLARELGVHASHAGARQEQAHVV
                     G"
     gene            complement(14037..14342)
                     /locus_tag="PKN51_07380"
     CDS             complement(14037..14342)
                     /locus_tag="PKN51_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNT84958.1"
                     /translation="MNVFQEFRRCRDLGTLRSRVTELSARFGALSRLDILTAVHAGRQ
                     QAICFLRMQTAEQERRLMKTLGVGRFGGEIVFVVDLPEPTGFDDSLPSSQWADSEVQ"
     gene            14665..16725
                     /locus_tag="PKN51_07385"
     CDS             14665..16725
                     /locus_tag="PKN51_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNT84959.1"
                     /translation="MPDAARHLRARLMRALQVGEWSYDPATQRFWLSGDAAALLGVVP
                     GSITVEHLWPRLAEESAQRLARALLSCQQGGAPIDVEVQLAPGEEPRRWLRVVGEADG
                     AATAAGTAIQGAVQDVSSRKQAQEETLRLAMRLTTTLASITEAFVTLDRAGRFMYVNE
                     ESERLLGASSRELLDEPIWQHLQGQQPGLLQARVHEALRQDQRVEFEDFYPAAGKWIE
                     LRAYPYAEGLAVYLRDVSERKAAEAKIHHLAFYDPLTGLPNRQLLIERLEAARAACAG
                     QDPTGALMFIDLDHFKVLNDTRGHHTGDLLLRGVAARLRGSVREGDTVARLGGDEFVV
                     LLPALDRDPALAARQAQAVARKVLARLIEPFDLDGHRHHCTTSIGVTLFGAGPDSVAE
                     LLQQADLAMYQAKHMGRNTVAFFDPAMQAALNASTTLAADLRAALGAERQLELHFQPQ
                     FDRARRITGVEALLRWQHPQRGLVGPTEFIPVAEDSGLIVPLGLWALTQACRQLARWQ
                     TQPHTAALSVSVNVSAQQFRHPDFVDQVTAVIRQSGAPAARLRLELTESLLADRLDIT
                     LAQMDRLRQLGVALSLDDFGTGYSSLAYLKRLPLDQLKIDRAFVADVLTDSGDAAIAS
                     AIIAMAHSLGLKVVAEGVETEAQQRLLSQRGCDGFQGFLLAPPMPAPQVEHFIARHTT
                     AGAA"
     gene            complement(16889..17839)
                     /gene="rocF"
                     /locus_tag="PKN51_07390"
     CDS             complement(16889..17839)
                     /gene="rocF"
                     /locus_tag="PKN51_07390"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNT84960.1"
                     /translation="MTAPAPPPAITLIGAPTDVGGGARGASMGPEALRVAGLVEALRA
                     LGADVQDAGNLAGPPNPGLPPVQGFRHLAEVTAWNQAVHQAVQASLQDGRLPLLLGGD
                     HSLAIGSISAVARHCRARGQPLRVLWFDAHADFNTAALTPSGNLHGMPVASLFGDGPP
                     ALVGLGGFSAAQPALRAHELRQIGIRSVDAGEKRLVHEAGIEVFDMRAIDELGMRAVM
                     ARCLAGLDAGSHLHVSLDVDFLDPDIAPGVATTVRGGPSYREAQLCMEMVADTGRLGS
                     LDIVELNPACDSHNRTAELAVDLVESLFGKSTLMRQRGRG"
     gene            17965..18549
                     /locus_tag="PKN51_07395"
     CDS             17965..18549
                     /locus_tag="PKN51_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HNT84961.1"
                     /translation="MWLLVLGLLLFLGVHSVRIVADGWRTRGIARLGAGRWKGLYALL
                     SLLGLALIVWGWALARRQPVVWWVPPAGLRHLNALFTLAAFVLLAAAYVPGNLVKARL
                     RHPMVLGVALWALGHLLASGSGAGLLLFGAFLLWALLDWRAAMRRDRAQGTAAPPGRW
                     GATLLTLLVGALAWVAFAFWLHAAWIGVAPLGAL"
     gene            18560..18832
                     /locus_tag="PKN51_07400"
     CDS             18560..18832
                     /locus_tag="PKN51_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT84962.1"
                     /translation="MTAAPRRAALPQIDPTRCTGCGWCVAVCAPHVLSLHADGGRKAS
                     ALDDAAGCTGCALCALRCPFGAIRMRRRPAQAPEAGPASDAGRISN"
     gene            18890..>19207
                     /locus_tag="PKN51_07405"
     CDS             18890..>19207
                     /locus_tag="PKN51_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNT84963.1"
                     /translation="MLPIENPSTHDTTRIDDTRIRAVRPLLTPALLEEWLPAPDAAQD
                     LVEASRTALSRVLHGQDDRLVVVVGPCSIHDHDQAMDYARQLKVQAEALQDDLLVVMR
                     VYFE"
BASE COUNT         2797 a         6526 c         6924 g         2960 t
ORIGIN      
        1 caggatgagc ggcgcggggc gcaggcttgc gcccggtgag gcccgcttga cggccggtgc
       61 gtaccccgca gcctggcgcg gcctcagcct gaccacgccg gggtgcgcaa ggcttcggcc
      121 agaaagtcga tcagcgcgcg caccttgggc gtcaggtgct tgcgcgtcgg atagaccgca
      181 tagatgccga tctcgggcga gcggtattga ggcagcaccg gcaccagcgc gcccgaggcc
      241 aggtgctgcg ccaccagaaa ggtcggctgg aacacgatgc cctgatgccc gatcgcggcg
      301 gcgcagcagg tgtcgccgct gttggtgcgc atgcgcgggc tgatcctgac gttgaggctg
      361 ccttcgggcc cttgcagctc ccactgttcg ccggtggaca gcagcgtgta ggagatcagc
      421 gcgtgctgct ccaactccgc cgggtgcacg ggtgcgccgt gctcgcgcag gtattgtggt
      481 gacgcgcaca gcaccatgtg ggtgctggcc agccgccggc tgaccagcga tgaagcctgc
      541 agccgcgcaa tccgcaccgc caggtcgtag ccctcgtcga ccagatcgac gaagcggtcg
      601 gacagcgtga cgtcgagcac cacctggggg tgctgcgcca tgaagcgcgg ccacagcgac
      661 gccagatgca gcagcccgaa ggtgaacggc acgttgatct tcaggcgccc gatcaccgcg
      721 cccgagtggg tgctgagctc ggcctcggcc tcggccacgc tgtcgagcag ctgccggcag
      781 cgctcgtgaa acacctcgcc cacttcggtc agcgacagct tgcgcgtggt gcggtgcagc
      841 aggcgcacgc ccaggcgcgc ttccagatcg ctgaccaggc gcgacaccgc ggccttggac
      901 agatccagcg catcggccgc acgcacaaag ctgccggcgt ccaccacggc cacgaacacc
      961 cgcatttcct gaaacttgtc catcgcccat tgttccgatg atcggaacat tttgtcaatc
     1021 gatcggcggt ttatcaaaga ttctctgaac cataaagttc agtccatcgc agcgatccgc
     1081 tgtatttcac tgaggaacag atcatgaaag tcaccgtcat cggcgccggc cacatgggtt
     1141 cggcattcgt caagcaactg gtccaggccg gccacgaggt cagcgtcacg gcgcgcagcc
     1201 gcgccaaggc cgagcggctc gccgccgccc atccgggcgc caaggcggtg cctgccgcaa
     1261 cggctgccgc cggcgcggat gtcatcgtgc tggccacggg ctacggcgac gccgtggagg
     1321 cactcaagtc cgtgggcgac ctgcagggca aggtggtgat cgacatcacc aacccgctga
     1381 cggccgacta catgggcctg acgctgggtc acagcacttc ggctgccgag gaaatcgcca
     1441 aggccgtgcc gggggcgcag atcgtcaagg gcttcaacac cgtgtttgcc caggtgctgg
     1501 ccgaaggcgc ggacttcggc gccggccgca gcgtcacggt gttcgtcgcc agcgacagcg
     1561 agcgtgccag gcagaccgcc agcgcgctgg cgcacagcat gggcttcgag gtggtcgatg
     1621 ccggcggcct gaagaacgcg cgctacctgg agccgctggc gggcctgaac atctacctgg
     1681 gctatggcgc cgggttgggc acggccattg cgccgacctg gatccgcaag gcttgagcca
     1741 ccccatccgg agacatctca tgagcgaatc cctcatccaa cgcttgctgg ccacaggcaa
     1801 cggcagcgct gccctggtcc tgcgtgtccc ggtgggcatc atctttgcgg cgcatggcgc
     1861 gcaaaagctg tttggctggt tcggcggcca tggcctggaa ggtacgggcc agttcttcgc
     1921 ctcgatcggg ctggcgccgg gctatctcat ggccctgctg gcgggcgctg cggagttttt
     1981 cggcgggctg gccttgatcg tcggactgct ggtgcgcccg gccggcgcgg cgctggcctt
     2041 cgccatgctg gtcgccatcg tctcggcgca tgggggcaag ggcttctttc ttgccagcgg
     2101 cgggctggag tacgcgctgg cgctgctggc ggcggcgctg tcgctggcct tcagcggggc
     2161 cgggcgctgc tcgctcgatc gcgcctttgc acgctcacgg tcctgagcct ccaatatggc
     2221 cctggcgctt gctgggcgtg cgccaacagc tatacatacg atagctatct gcggcctgaa
     2281 gtcggggccg ccgggccgcc ggcgttgtcg gccacccagg cggcgtaggc ggggtcggcc
     2341 gccacggtgg cgatggcgtg gatcgccggc agctggtagg ggtggtgctg gcggatggcg
     2401 gcttcaagct gcgcgtactg ggctggctgg gtcttgcaca gcaggcgaaa ttcgccgtct
     2461 tcgagcacct cgccctgcca gcggtagatg ctgtcgatgg cttcgatctg cacgcaggcg
     2521 gccagccgca ggtgcaccag ggtgcgcgcc agcgcctgcg cctgctcgcg cgagccgacg
     2581 gtggtgacca ccagccaggg caagggcgcg gccatggccc ggcccgcctt caggctgggg
     2641 cgcctgccgg cgccggatgg cccatgcggt cgagcgccgt cgccagctgg ccgacgctgc
     2701 gatcgacgtt ggcccatttg tcgaggccga acaggccgag acggaaggtg ctgaagtcgg
     2761 ccggctcgtc acacatcagg ggcaccccgg acgcgctttg caggccctgc tcgatgaact
     2821 tcctgccgtt ctggatgtcc gggtcgcggg tgtagctgac caccaccccc ggtgcttcaa
     2881 agccgggcgc ggccacgctg ggaaagccgc gcgccgccag cagcgcccgc accttgtggc
     2941 ccagttcgag ttgctgctcg cgcacctggg caaagccgcg cgcacgggtt tcggccatgg
     3001 cgtcgcgcag ctccagcagc gccatggtgg gcagcgtggc gtggtaggcg tgggcgccgg
     3061 tttcgtaggc ccgcatgatc tggtgccact tcttgaggtc gcacgaataa ctggtgctgg
     3121 tgcggctttc catcacctgc agtgcgcgct ccgacaggca caccatggcg caggccgggg
     3181 cgctgctcca gcccttctgt ggcgcgctga tcagcacgtc gacgcccagg gcctgcatgt
     3241 ccacccacat ggcgccggag gccacgcagt cgagcacgaa caggccaccg acggcgcgcg
     3301 tggcttcgcc cagccgccgg acgtagtcgt cgggcagcat catgccgctg gcggtttcga
     3361 catggggagc gaacaccacc gccggccgtt cgtgcgcgat cgctgccgtc acttcgtcga
     3421 tcggtgccgg cgcccagggg gcttgcgggc cgtcggcggt gcggcgtgcc ttgaacacgc
     3481 ggatgtgttc gggcgcggtg atctgcccca tctccagaat ctggctccag cggtagctga
     3541 agaagccgtt gcgcacgatc agcactttct gcccggtggc gaactggcgc gccaccgcct
     3601 ccatgccgaa ggtgccgctg ccgggcacga tgacggcggc gtcggcgtgg tagacctgct
     3661 tgagcgtggc cgcgatgtcc ttcatcacgc cctggaacct gacggacatg tgattgagcg
     3721 cgcggtcggt gtagaccacc gaaaactcaa gcagaccttg cgggtcgatg tggggcagca
     3781 gtccgggcat ggggttctcc tgatcggttc gcgggcacgg gcgacgcgcg gccggcaaaa
     3841 aaatcagctt agcatgcgcc acccgtgcag gccacgcggc ctgcatggcc gaggtgcggc
     3901 gccccgaacc ggcagccgct gcgcgcgcgg ggcgggtgca gccttggcgt aacatcggcg
     3961 ccaccatgga cattcccgcg ttcgccctgg cgttcggcaa gagcctgctg ttcgccctgg
     4021 cggccgtgct gccgatcctc aatccgccgg cgtcggcgcc ggtgttcgtc aacctcacgc
     4081 gcgggctgga ccatgacacg cgcgccatgc tggcgcggcg catcgggcgc catgtggtga
     4141 tgctgctggc cggcgccatg ctggtggggt cgtacgtgct cgacttcttc ggcatctcgc
     4201 tacccatcgt gcgcgtggcc ggcgggctga tcgtggcctc caccgcctgg cgcatgctgc
     4261 atgtccagca tctggcgcag gacgacaagg cgcagctggc gcagtcgctg acggccgaca
     4321 acgtgcgcat ccaggccttc tatccgatga ccttcccgct cacctgcggg ccgggctcga
     4381 tcgcggccgc catcaccgtc ggcgccagcc tgcacagccg ccgggtcacc gaggccctgg
     4441 tcaactttgc cggtggcctg gccgccaccc tgctggtggg cctgctggtg accctgacct
     4501 ttcgctacgc cggtcgcctg ctgcgccccc tgggcgaggt cgggcagatc gtgttcctgc
     4561 gcctgatggc cttcatgctg ctgtgcgtgg gcgtgcagat cgtgtgggac ggcgccagcg
     4621 cgctgttgca gggcctggga aattcgaaat aaatcagcct cttgacggcg cctgaactgc
     4681 gccaacagct atcaaaatcg tagctttctg gattcaggcg ggccgcacac cggcggccac
     4741 cagatcctgg atcagggctt cgttcaggta gtgcgggcca tcgaaggcca ggatgggcca
     4801 atcctggtag gcgtcgagct gcggcaggaa ctcctgcgcc gtggcgctgc gaaagccccc
     4861 tcccggggcc ggtcggaagg cttcggcgat cacctgcacg cgggtgcgcc ccaaccgggc
     4921 accgagctgt atcaggtatt ggcgtgccag cgccggctcg cgcgcatgca cccccacgcc
     4981 gtcctgaaac agcaccccca catcggtcgg cagccagcgc tcgatccagg ccagcagcgc
     5041 atgcggcccc aggttggcgc tgtcgtacac gctcacccac agcggccgcg gcagggcgtt
     5101 caaccgctcg ggcaggtcgg ccggcggttg ccagccgggg tcgatctcga ccgggaagta
     5161 ccagccaccg atgcgcagcg gccacggcgc ggcagccacg cacagcgcgc gcgactgcgc
     5221 caccagcgcc ggcagggcgg cgcgcgcgag cggctcggcg tgcagcccgg ccaggccgac
     5281 gatcacctcc tgtgcccagg gctcgccggc gatccggtcc cagtccggca gcggcggcgg
     5341 ttgcagcgca aggccggcat ggggcagaaa gctcaggttg tccaccgccg tccactgcac
     5401 cagcagccgg cgtatgccca gctgggccca gttgccgcgc ggctgcaggg tctggcggct
     5461 gggctgccag accatgccgg tcaggcggcg cgccgcaggc gccgccgcag ccgaccacgg
     5521 cagcatcgcg gccgccagca cctggcgccg gctcaggtgg cggccgcggg gcggcgtgcg
     5581 cggcaggtcg gtgtccatgt gaagacccgt ggcatcacag ggctgccacg ggcgtcgatc
     5641 ggtggccact ggccatgcgg cccgcccgcg gcgcgccgtg gcagggtgct cagttgatct
     5701 ggctcggcac ggtccaggtg aagccgagat tgctgatgcc ctgaatcacc ttcttgaagt
     5761 ccgccaggcc cggcacgccc agggcgggct cgagccagaa cggatggaag aagaacgacg
     5821 catagccgtc acgcacggtc ttcacgtact gggcgttggt cacgatgtcc tgccacgtgt
     5881 agttgtagtt cgacgtcggg tcgatgctgc tgatgtcgta ctcgatgttg cccaggcttt
     5941 ccggcagcac ccgctgcccg tagtagtccg acttgatcgg gtagggaaag atctgcccca
     6001 ccccgaagtc cttggccacc ggggcgttga agttgggctg atcggcggtg aagtacacca
     6061 cgcgctgata ggtcttggca aaggtctggg cggccgcctt ggacgccagc gccgagccgt
     6121 ggtagtgcgg cgtttcccag gccgtcgccg tgtagccgtt gctggacagc tcctgcagcc
     6181 cggcctggta gcgcgacagc gcccaggcca gcgagtcttc catcaccggc gtgttgtcga
     6241 cgatgttcca gaactcgtag tcgtcaccgc tcacgccggt gtatttgttg cgcatgttgc
     6301 tgtactgatg ggtgtagcca tgcatcacga tctcgccgcc gcgcgccagc gcgtagttga
     6361 gcgaggtctt caggttgctg gcctgcgaca tcggcacgaa catgggcacg ccgccgttgt
     6421 aggtgcccag cggatcggcg tagtagggaa tggtggcgat cgagaacggg atgcgccgct
     6481 ggtacaggta gttggtgagc gatttcatgg cgctcacgct gaccatggcg cccacgtctt
     6541 ccagtcgcac catggccttg tggctggtgg cgtggttgac ccccagcatg tcgtgcagca
     6601 gatcggtcag caccaggtag cgatcgcgcg ggccgatgta gctgaacggc aggtcggcca
     6661 cgtaccagaa gttgccggcc cgcacgatgt agggggcggt ttcgctggtc ttggggttgg
     6721 acatcggcac cagggtggtg gccttttggg tgtcggtgac ggcgacgatg cccacatccg
     6781 gatcggcggc caccgcgcca cccgcatagt cgtagtactt gacgaagggc agtgacttgt
     6841 aggtgatggt gtcgaagaag ccgggggctg gattggccgc cgacggcagg gcgttcaggc
     6901 cgcgcagccc gctgaacccg aagccgaagc ggttctggaa gtcgtaggcc gggttccagg
     6961 cgatctgcca caggttgtac ttgaaccaga ccacggtctt ggtggtggtg gtcacgtccg
     7021 ccaggaaggc cggcggcacc tggttgtcgt agtaggcgcc gaggtagaac gtggcgtcgt
     7081 agttgttgac ctgcccggtc acgtagtcgt gcaccggcac catgtcgacc tggctgtcga
     7141 agtgccccag caggttgcgc atcatgatgg cgtaggccat gcccagcttg gtgaaggtct
     7201 gaccgggcgg ggcgtcgtag agcaccaggg tgcgcgggcc ggcttgaggt gcaacgggcg
     7261 gcggtgcact gggcgcgatg agccagggca gtctgatgga gatgcgaaat ccggtgtcgg
     7321 ctgcggtcgc cgccgtcatg cacagggcgg acagcagtgc aacgaagtat ctgagggttt
     7381 tcacgatcgg ctcctgggtg tgttcgtgac gtgaccaagc ggtctatttc gaatcgcggg
     7441 gcaccgaaac ggtcgcccgc ggtatggcgc cttgacgtgg cggggccagc gagtcatcgt
     7501 gcaggtgatt gcggcgcagc acgctgggct gcttgtgcgc cttctcggcg cgcttttcct
     7561 cggtgggtga ggcgccgacc tggggcttgg ccatcgccga gcccatgggc agcggcggcg
     7621 gtgccacggt ggtggcgctg gcggcgaggc tgcacagcag cagggtggcc gaaacaccgg
     7681 cgcgcagtgg atgggtgctg agcgttttca tcgtcgatcc cctttcagtt gtcaatcaaa
     7741 cggaacatga gtggctgccg tgcgcgggcg aggccggatc agaagttgag ggtggcgcgc
     7801 agaaacacgc ccttgccacg ggcgtcgcca cccagccggg cccggtactg cagcgagacg
     7861 tcgaggtagg accgcggggc ctggtagcgg tcctggttga accagcagcg cgccgccaca
     7921 ccgatgccgg cgccgctggc gctgcgctgg ccgggggcca gcttcgagtc gtgatcggcg
     7981 gccagcacca ggtgcggaaa cagcaccagc tgggcgtcga tgtcgttcag ccggatgctg
     8041 cgaccgaggt tcgcctcgaa ggtggcgtag cttcggtgcg cacgcaggaa ccgtcccgct
     8101 tcggcataga cctggacggt gaaccagctg tcggcatcga tacgcaggtc ggtgccgtgg
     8161 gtgtacgagt agccggcgcg cagcagccag tcgttgctgg acagccgacc gaccttcagg
     8221 cgccgctcga tcgccagcac caggttgagc gacgacagcg gtttgacacg cgcgcccagc
     8281 atggcctgcg tggtgggccc gcccgacggc gccgcaccct tggcccaggg ggtctggaag
     8341 cctccgccgt agagctcgta gtagctgccg tcgcgatagc cggccgggcg ccagtacagc
     8401 tccatccccg cctgcaggct gtcgcccagg gtggtgggcg tgcccgccat ctgcccgccg
     8461 ggggaaacgc cgcggtagcc cagcgacagg atggcacccc aggtgcgggt gcggtccgcc
     8521 acctcgcgcc gcgtctcgaa cagccgttgc ggtgtcaggg ccagccgacc cgcctcggcc
     8581 gcgtcgatgg cgcgcttgaa gtagtcgacg gcgacctggg agcggcccag ccggccagcc
     8641 acgtagccgg catccagcaa ggcgtggtcg ggcagctgtc catgctgggc ggcgcggtca
     8701 aaggcctggg cggcactggc gtcgtcgccc acccgcacgg ccaggtaggc ggcgtcggtg
     8761 tcgctcagct cacccatcat gggcgagtgc tgcgcggcgg cgaacacggc gcgcgcctgc
     8821 gccggatcgc tgggcagcag cagatcgatc tggctcgata ccgacaacct ggggtcggcc
     8881 agcgcggcgc gcgcgtcggc cgccgcggcc tgcggatgct ccagttgccg atgcagggcg
     8941 ctgcgcaggg ccagcagctc cggttcaggt tcgccgtggg acaggaattc gttggcgcgc
     9001 tccagcgcct gctgcggctg gtcggcggcc agcagggcgc gcaccagcag cagttggtag
     9061 cgccggttgg cgggcgattg ctcgacggcg ctgcgcgcct gcgtcgccgc caccgcatag
     9121 gaccgtgccg cataggcgcg cagcgcggca tcggcatgga cggcgcccgg atcggccggg
     9181 gcgtcgcccg gccacagctc gcaatagggg gtgtgcgccg agccgacgca gaccaccctg
     9241 ggcgtggcca gggcgggcac cacggtgggg ctgggcagca gggtgcggcg cgcgccggcg
     9301 ctggcctgcc ggagccgggc gtcgacagcg gcgtcggccg gtcccgacag cgcggccagc
     9361 agctgctcgg cacgcaccgt ctggccggct gccagggcgg cgtcggccac gatcaggcgg
     9421 gcgttgcggc tgcgcgtgtc ggggtcggac gagagcgcca gggcgcggtc gtagtcggcg
     9481 ttggcgtcgt ccaggcggcc cagacgctgg cgcgcatgcg cgcgcagcaa ccggatgtcg
     9541 gcgtcgtcca tgccaggacg ttgcagcacc tgactggccg cgtcgtccag cgccgaccag
     9601 cgctgcgccg ccgccagggc acccagccac tgcacctgct gagccagtgt ggcgggcgcg
     9661 agctgggcgc cgcgcgccgc ctgcgtgacg gcgccatcca catcgccggc ggccagcgac
     9721 cggctggccg cctcgaactc gacgtaggcg cgctgccgac gcgcctgatc ggccagcgcc
     9781 accatggcgg ggtcggagct ggagcgctcg ccggccagcg cttccacgtc ggcgtaggcg
     9841 ccggtttcca gcaaggcgta ggccagcaga cggcggtagt cggcgttgtc cggctgcagt
     9901 tgcagcgcct ggcgtgcgtc acgcgccgcg gcggcaaagt cgcccgtggc gctggccttg
     9961 taggcggcgt cggccagcgc gtaggccgcg cgggcgccgt gctgcggcgc ctggttggcc
    10021 ggtggcatgg caggcggggc cagcgcggcc aggcgtgtgg tggctggggc cgagcgtgac
    10081 ggcgggcggc cgccggccga tgacgcccgg tcacgacggc tggtctggcg tgcgtcggcc
    10141 gatgcggcat cgggggtcgc cgcagcgcgc gcgcgtggcg gcgcggcctc gaccgcaggc
    10201 gcaggcgctg gcgctggcgc tggggcggcg gcaagctcgg ccgcccggtc gcccgccccg
    10261 cccgccgtca cctgcggtgc aggcggccgg gtcggcgcgg ccgggccgag gctggccagg
    10321 gcctgacgca ggcgggggtc gtcgggatgg cgggcgagct gccgctgcag caactggcgc
    10381 gccttgtcgt ggtcaccgaa atggcgatag gcttcgacca ggtagcccgc gatcacggcg
    10441 ctgtcgggcg ccagtttcag ggcctgctcg aactcttcgt aggcaagggt ctgctgggcc
    10501 tcccgcaggg cctgaaagcc cttttgcagg tgcggataga ccagaaatcg ggtgtagccc
    10561 gacacccccg gccccagatc gaccggttga cccagcgccg gcagcgcggc cagcagcagc
    10621 gtcagggcca gggagggcga caggcgcttc attcatgcct ccgtcgccag cgggccgctg
    10681 ccgacgcggc ccggcaccag gccgcccagc aaggtgtcga tcgagggctg gagcgatcgc
    10741 tgcagtgcca gtgcctcgtc gagcacctgc ggcgtcagca gcccacgcgc caccatgaat
    10801 tcgcccagac gctgcccggt cttggcgcgc tggatcagca ccgatttcag cgccgccgga
    10861 tcgacatggc ccagcgcaca caggatgtcg cccagcagca gctgtccccc gacatagacg
    10921 cgccacatca gcgccgcggt ctgcggtgtg gcatggacgc cgccacggcc tgcatccgcg
    10981 gcgaccagat cgccgtcacg gccataccag cgtcgcagcc ccaccgtgac ctcgcccttg
    11041 ggcacgatca cgtagctgac cttgcgatcc agcttgcggg cgatggcggc gagcgaaacc
    11101 gggtcgaggt acgactcgca cgccagcacc agcgtgtcct cggtctgccg catcggcagc
    11161 acggcatagt gcagcgccac cgccgcaggc acgcaggcca ccagctcggg gccgagctga
    11221 cggctgtcga aaggctccca gtcggcgcgc gactgcgtgg ccaccgcctg ggcgagctgc
    11281 tccggggtga ccaggccgcg atgcaccagc atggccccca gccgcagccc cggcaccggc
    11341 tcggccagcg cttcatcgag ctgttcctgg gtcagtgccg actgcgccac caggatctgt
    11401 cccagcggct cgcgggcgcc gccttcatcg cccagggtgg ggaagtcgtg ctcggtcttg
    11461 gcccaggcga tgcgccgcac atcctgggct tgcagggtct gcatcagcgc gcgccagttg
    11521 gcaaagaaat tgatcacgtt gccccaggcc atgcgcggca gcgacagcag gccctgccgg
    11581 atgccgtagt aggacgacac gaaatagatg cgctgcacgg cgcggttgca cagcagcacg
    11641 agattggcca ccagcaggag cgtcagccag gcgctgtctt cgaagatgga gagaaactga
    11701 tagctgtcgg tcaccagccg ctggtagagc cacagggcca ccagctgcag cagcaccagg
    11761 gtggccagca tgccggcgaa gttggtgatg acgcccttgc ggtcgcgcca caggaaatag
    11821 ttcaggcccc agtgccgtga ccaccccatg gtcttggtgc cctggtagat gatcccgatg
    11881 atccagcgcg atttctggcg aatggcggca cgggtcaccg acgggaagta ttcgcgcacg
    11941 cacaccgtgc tggcctcgcg ggcgttctgc ccgagcgggg cgccggcgtc gcgcgcgtgg
    12001 tggggcttca tcgggaagcg cacgaacacc tcctgcatgc ccatgccctt caggcgcagg
    12061 ccgatgtcgt agtcctcggt caggctcttg acgtcgaacg caatgccctt gccgtggctg
    12121 agcagggcca tcacggcctt gcggctgaaa caggtgccga ccccggcgct cggcacctgg
    12181 ccgaccaggg cctcgcgcac cagcagatcc ttgccatgcg actcggcgaa ttcgtccagg
    12241 tagtggcagc tggtgaagtc ggtccacggg cgcggcagcg ggtagaccgg caactggatc
    12301 aggtccttgc ggcccaccag atggttgaac acccgcagct ccatcggcga cagcacgtcc
    12361 tccgaatcgt gcaggatgaa cccctcgaaa tggatgccgg cgcgccgctc gaactggaag
    12421 atggcgtcca gcacattgtt cagacagtcg gctttgctgg tcgggccgtc ccgggcgccg
    12481 accaccttgt gcacgttcgg gaagcgggcg cacaccttct cgacgtccga ctgcgtctgc
    12541 gggtcgttcg ggtaggtgcc gacgaagatg tggtagttct cgtagtccag cgtggtggcc
    12601 gccagttcgg ccatgcggtc gatcacccca tgctcctgcc aggccggcac catgatggcc
    12661 atcggctttt cgtcgcgcgc ctgcagatcg gccggtcgcg cgcgcgcgtg gcgccggtag
    12721 atggtggcgc tgcgccacag gcggcggcac cagtaggcca gatcgatgaa caggtcgtcc
    12781 aggctgctga ccaggatgat cacggccagc gccagggcca cggacttgag cccgtacaga
    12841 tagctcgaga cgtagtcaac ccaccacatg cgcctgctcc tgtcgggcac cggcatggct
    12901 ggcgtgaacg ccgagctcgc gggccagatg gcgggcgatc tgccgggacg ccgtgccgtc
    12961 cccgaacggg ttgccggcct gctgcatgcg caggaactcg gccggctcgc gccacaggcg
    13021 ccgcacctgt tccacgatgg tgtcggtcgc ggtaccgacg atggccgcgc agcccgcttc
    13081 cagcgcttcg gggcgctcgg tttcctcgcg caacaccagc aggggaatgc cgaaggtcgg
    13141 ggcctcttcc tgcagcccgc ccgagtcggt cagcacgatc caggcgtcgc gcagcgcctg
    13201 ctgcatttcc agatagtcca gcggcgccgt cagccggacg ttgggcagcc cggccagcaa
    13261 ctggtgcacc ggcccgtgca ccaccggatt caggtgcacc ggaaacagca cctccagatc
    13321 gggctgttcg cgggcgatgc gggcgatggc gcggcacacg cgctcgatgt cctgccccca
    13381 gttctcgcgc cggtgcatgg tcaccagcag gtggccacga ccgttgaccc ggcggccgat
    13441 gccgcggtgt gcgcacaccc attgctgggc atcgatgacg gtgttgccgg tgacccagat
    13501 gcgctgctgc ggcacgccct cggacagcag cgcctgccgc gaacgcgccg tgggcgcaaa
    13561 gtgcaggcgc gcaatgcggc cgatcatctg gcgcagccct tcttcgggga agggccggtt
    13621 caggttgtag gtgcgcaggc cggcctccac atgggccacc ggcacgccgt ggtagaaccc
    13681 cgccagcgcg ccctgaaagg cgctctcggt atcgccctgc accaccaccg ccgcccagcg
    13741 gccgcccgcc agcaccgcct ccagctgggc gcggcagccg aggctgaagt cgagcagcga
    13801 atcgccctgg cgcgtcaggg tgaggtcggg ccggatgtcg aagctgtcca gtatgggctg
    13861 ggccatctgc gcgtgctggc cggtgtgcag ccaaaccaca tgggcccagg gctggcgcct
    13921 gagttcgtgg tagagcggtg ccagcttgat gatctccggc cgggtgccac agacgatcag
    13981 gatcgaggcg gcggcagcca caccgccgcg gttgcggtcg cggacgcggg cgtccatcac
    14041 tgcacctcac tgtcggccca ctgcgacgag ggcagcgaat cgtcgaaacc cgtcggttcc
    14101 ggcagatcga ccacgaacac aatttcgccg ccaaagcgcc cgacgcccag cgtcttcatc
    14161 agcctccgct cctgttcggc ggtctgcatg cgcaaaaagc agatggcctg ctgtcggccg
    14221 gcatgcacgg cggtcaggat gtccagacgg ctcagtgcgc cgaacctggc gctcagctcg
    14281 gtcacgcgcg acctgagggt gcccaggtca cggcagcggc gaaattcctg aaagacgttc
    14341 aatttgtatc tccctgcggt cgcccgcaac ggatcgaggc gacgtgtttc agactatttc
    14401 ggaagcgccg ctccggagca attcacccgg attggggttg ccggaccgcc gcggggcgaa
    14461 cgcaacgtgg cgtcagcgct ggcgcatccc ttgatcttgg cgacatgcaa ctgaacgaaa
    14521 caatcgatgc attgcgcgac aaagaggttc tggcggatga tccgcacatg aggtacgcgc
    14581 tcgtcctggc ggttgcgacc ggtcatcggt cggggtggcc gcgcgaccgc ggcaggccgt
    14641 ggtgtcgttg cgcgcgaggc gcgcatgcct gacgcggcgc ggcacctccg ggcgcgcctg
    14701 atgcgggcgc tgcaggtggg cgagtggtcg tacgacccgg ccacacagcg gttctggctg
    14761 agcggcgacg cggccgcgct gctgggcgtg gtgccggggt cgatcacggt cgagcacttg
    14821 tggccgcgcc tggcggaaga atcggcgcag cgcctggcgc gtgccttgct gagctgccag
    14881 cagggcggtg cgccgatcga cgtcgaggtg cagctggcgc ccggcgagga acccaggcgc
    14941 tggctgcgcg tggtgggcga ggcggacggc gcggccaccg cggcaggcac ggcgatccag
    15001 ggcgcagtgc aggacgtgag ctcgcgcaag caggcgcagg aagaaaccct gcgcctggcg
    15061 atgcggctga ccaccacgct ggccagcatc accgaggcct tcgtgacgct ggatcgcgcg
    15121 ggccgcttca tgtatgtgaa cgaggaaagc gagcggctgc tgggcgccag cagccgggag
    15181 ctgctggacg agccgatctg gcagcacctg cagggccagc agccggggct gctgcaggcg
    15241 cgcgtccatg aggcgctgcg gcaggatcag cgcgtcgagt tcgaggactt ctacccggcg
    15301 gcgggcaagt ggatcgagct gcgcgcctat ccctacgccg aggggctggc ggtgtacctg
    15361 cgcgacgtca gcgagcgcaa ggcggccgaa gccaagatcc accacctggc gttctacgac
    15421 ccgctgaccg gcctgcccaa ccgccagctg ttgatcgagc ggctggaggc ggcgcgcgcg
    15481 gcctgcgcag gccaggaccc gacgggggcg ttgatgttca tcgacctgga ccacttcaag
    15541 gtgctcaacg acacccgtgg ccatcacacc ggcgacctgc tgctgcgcgg cgtggccgcg
    15601 cgcctgcgcg gcagtgtgcg cgagggcgac acggtggcgc gcctcggcgg cgacgagttc
    15661 gtggtgctgc tgccggcgct ggaccgcgac ccggcgctgg cggcgcgcca ggcgcaggcg
    15721 gtggcccgca aggtgctggc gcgcctgatc gagccgttcg acctcgacgg ccatcggcac
    15781 cactgcacca ccagcatcgg cgtcacgctg tttggtgccg gcccggacag cgtggccgag
    15841 ctgctgcagc aggccgacct ggccatgtac caggccaagc acatggggcg caacacggtg
    15901 gcgttcttcg atccggcgat gcaggcggcg ctcaacgcca gcaccacact ggcggcggac
    15961 ctgcgcgcgg cgctgggtgc ggagcggcag ctggagctgc atttccagcc ccagttcgac
    16021 cgcgcgcggc gcatcaccgg tgtcgaggcc ctgctgcgct ggcagcatcc gcagcgcggc
    16081 ctggtggggc cgacggagtt catccccgtg gccgaagaca gcggcctgat cgtgcccctg
    16141 ggcctgtggg cactgaccca ggcctgccgc cagctggcgc gctggcagac gcagccgcac
    16201 acggccgctc tgagcgtctc ggtcaacgtc agcgcgcagc agttccgcca cccggacttc
    16261 gtcgatcagg tgacggccgt catccgccag agcggcgcgc ccgccgcgcg cctgcggctg
    16321 gagctcaccg agagtctgct ggccgaccgc ctggacatca cgctggcgca gatggaccgc
    16381 ctgcggcagc tcggcgtggc gctgtcgctg gacgacttcg gcaccggcta ctcgtcgctg
    16441 gcctacctca agcggctgcc gctggaccag ctcaagatcg accgcgcctt cgtcgccgac
    16501 gtgctgaccg actccggcga cgccgccatc gccagcgcca tcatcgccat ggcgcacagc
    16561 ctgggcctga aggtggtggc cgaaggcgtg gagaccgagg cgcagcagcg cttgctgagc
    16621 cagcgcggct gcgacgggtt ccagggcttt ctgctggcac cgcccatgcc ggcgccgcag
    16681 gtggagcact tcatcgcccg ccacaccacc gcgggcgctg cctgagcagc cttcggcaac
    16741 cagtcatttg ctatcacaac aatagctgct ggcgcattct ggataagcgc cagagtctga
    16801 tttgatgctt gaacctggaa accgcagttg acaagcagtc aactgccgtt tccaggttca
    16861 tcagccacgg cctgcgcgcg cagccacctc agccgcgccc gcgctggcgc atcagcgtgc
    16921 ttttgccgaa caggctttcc accagatcca ccgccagctc ggcggtacgg ttgtggctgt
    16981 cgcaggccgg gttgagctcg acgatgtcga gcgagcccag gcgcccggtg tcggccacca
    17041 tttccatgca cagctgcgct tcgcggtagc tcggaccacc gcgcacggtg gtggccacgc
    17101 cgggggcgat gtcgggatcg agaaagtcca cgtccaggct gacgtgcagg tggctgccgg
    17161 catccagccc ggccaggcag cgcgccatca ccgcgcgcat gcccagctcg tcgatggcgc
    17221 gcatgtcgaa cacctcgatg ccggcctcgt gcaccaggcg cttctcgccc gcatcgacgc
    17281 tgcggatgcc gatctggcgc aattcgtgcg cgcgcagcgc cggctgggcg gccgaaaagc
    17341 cgcccagacc caccagcgcc ggcggcccgt cgccgaacag gctggccacc ggcatgccgt
    17401 gcaggttgcc gctgggcgtg agcgcggcgg tgttgaagtc ggcgtgggcg tcgaaccaca
    17461 gcacccgcag gggctggccc cgggcccggc aatggcgcgc cacggcgctg atcgagccaa
    17521 tggccaggct gtggtcgccg cccagcagca gcggcagccg gccgtcctgc agggaggcct
    17581 gcacggcctg gtgcaccgcc tggttccagg ccgtcacctc ggccaggtgc cggaagccct
    17641 gcaccggcgg caggccgggg ttgggcgggc cagccaggtt gccggcatcc tgcacgtcgg
    17701 cacccagggc gcgcaaggcc tcgaccaggc cggccacgcg cagcgcctcc ggtcccatgc
    17761 tggcgccgcg tgcgccgccg cccacgtcgg tgggcgcgcc gatgagggtg atggccgggg
    17821 gcggggcggg ggcggtcaag acgatgctcc tggggcggcg ggcgtgcgcg atcttcgcac
    17881 gggcgccgcg ttcgattcca taatggttcg ctctgccatg ccggcgccgg gcccgcgcgc
    17941 ccggccgccc caaggaggtc tgcgatgtgg ctgctggtgc tcggtctgct gctgtttctg
    18001 ggcgtgcatt cggtgcgcat cgtggccgac ggctggcgca cgcgcggcat cgcgcgcctg
    18061 ggcgcggggc gctggaaggg gctgtatgcg ctgctgtcgc tgctcggcct ggcgctgatc
    18121 gtctggggtt gggccctggc gcggcgccag ccggtggtgt ggtgggtgcc gccggccggt
    18181 ctgcggcacc tgaacgcgct gttcacgctg gccgccttcg tgctgctggc ggccgcctac
    18241 gtgccgggca atctcgtcaa ggcccgcctg cgccacccca tggtgctggg cgtggcgctg
    18301 tgggcgctgg gccatctgct ggccagcggc agcggcgccg gcctgctgct gttcggcgcc
    18361 tttttgctgt gggcgctgct cgactggcgc gccgcgatgc gccgcgaccg cgcccagggc
    18421 acggccgccc caccgggccg gtggggcgcc acgctgctga cgctgctggt gggcgcgctg
    18481 gcctgggtgg cgttcgcgtt ctggctgcac gcggcctgga tcggggtggc gccgctgggt
    18541 gcgctgtgat gaccctggca tgaccgccgc gccgcgccgc gcggccctgc cgcagatcga
    18601 cccgacacgc tgcaccggct gcggctggtg cgtggcggtg tgcgcgccgc atgtgctgtc
    18661 gctgcacgcc gacggcgggc gcaaggcctc ggcgctggac gacgcggccg gctgcaccgg
    18721 ctgcgccctg tgtgcgctgc gctgcccgtt cggcgccatc cgcatgcggc gccggccggc
    18781 ccaggcgccc gaggcgggcc cggcgtccga cgccggtcga atatcaaatt gatagctggc
    18841 ggcgcttgct gcgcgctgcc tgcaatcgca aaacaccgat aatcggccga tgctcccgat
    18901 cgaaaacccc tccacccacg acaccacccg catcgacgac acccgcatcc gcgccgtgcg
    18961 cccgctgctg acgccggccc tgctggaaga atggctgcct gccccggacg ccgcgcagga
    19021 cctggtggaa gccagccgca cggcgctgtc gcgcgtgctg cacggccagg acgaccggct
    19081 ggtggtggtg gtcgggccat gctccatcca cgaccacgac caggccatgg actacgcgcg
    19141 ccagctcaag gtgcaggccg aggcgctgca ggacgacctg ctcgtggtga tgcgtgtgta
    19201 ctttgaa
//
LOCUS       DAOQTZ010000048        19095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3336_length_19095_cov_12.684821, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000048 DAOQTZ010000000
VERSION     DAOQTZ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 19095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19095
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3336_length_19095_cov_12.684821"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKN51_07410"
     CDS             complement(<1..369)
                     /locus_tag="PKN51_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT84964.1"
                     /translation="MIAGRLTTWAAAQHGARTAVVFGEQRYSHAEIEARSNRFAQAAL
                     ALGLQPGERLAVLLDNSVESLDSGFGAEKAGLTYVALNARHTLVEQLDILADAQASAV
                     LVGPRHGELAAAFAQAAGHQL"
     gene            complement(366..584)
                     /locus_tag="PKN51_07415"
     CDS             complement(366..584)
                     /locus_tag="PKN51_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNT84965.1"
                     /translation="MPRIHLPSEVTGTVWKVEVSAGDPVSADQTLVILESMKMEIPLT
                     APRAGVVLELLVAEGESVAEGQTVVVLE"
     gene            732..1721
                     /locus_tag="PKN51_07420"
     CDS             732..1721
                     /locus_tag="PKN51_07420"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT84966.1"
                     /translation="MQFRFLAGLMLGGLCLAATALAQTAWKPSERINYIIGVAPGGTV
                     DLYARAIKDSLERLKLTGGEPVIVENKVGAGGALAMNALRAGHGNAHYLGTFHTGAIA
                     GAVTGLIKADPREFMPVAMLVEETSVVAVRADSPLRSGTDLVAALKADPTALRIAVAP
                     ALGQNIHMAIAKPLHVAGVAIDKLTIAPFRSSGESVTALIGGHVDVVSATAPVVLPQV
                     ANGKLRILASAAPVPGSGPLAGVKTWREQGVPADYVSYNGVTLPPGVHAEQIRFWEEA
                     LRKVSQDPQWRALVEKSGNQPIFRGYVESHRYMMSEWDAAAELARTLGLKGGR"
     gene            1718..3301
                     /locus_tag="PKN51_07425"
     CDS             1718..3301
                     /locus_tag="PKN51_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNT84967.1"
                     /translation="MSAQQGQTLAPAGEWAAELAELQQRRASALAMGGAQALARMRDS
                     GRMNARERIAALIDQGSFHEMGTLAGKGHYDAQGRYSHTDPTNAIVGTGRIDGRKVAL
                     HVDDYSIRAGSSEGTIADKWIYIERLAHQLRMPLVRLVDSAGGSVKLLMQLGGTKIPE
                     YTTWPAQELLQTVPVVGVALGACAGQGAVKVLSSHFSVLVRGQAQVMAAGPHVVRQAY
                     GVEVDKNELGGHLAQRKSALVHNEAEDEADALAQVRRFLSYLPRSVHELAPVVACDDD
                     PHRADDWLKDAVPRDRRKIYDPRRILAAVFDQGSLFEIGRWQGGSLLTLLGRLNGVPV
                     GVIANDPKVQGGAMTLQAAYKMERHTRLCSQFGLPVVNFVDQPGNATGLEAELAGTLL
                     GAVRLGEALAHCRSPWVSILMRRCFGMAGALHGPKGGARLNHRFAWPSARWGSIPIEG
                     GVMAAHKAEIEAAPDPAARRAELEAHYLAMTSPFRTAEKFGVLDIIDPRETRRVLCDW
                     VEDAWYVVRGDLAQRSAGA"
     gene            complement(3381..4475)
                     /locus_tag="PKN51_07430"
     CDS             complement(3381..4475)
                     /locus_tag="PKN51_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT84968.1"
                     /translation="MNFAWPAFLKWPAAIPQPAFLWPHMLWLLALLPLLVLLYAWLLR
                     RRRKLALRYASLSIVRQAQGRGPGWRRHLPPLLLLLALAALLLASARPVAVIALPSNQ
                     QTIILAMDVSASMRATDVEPNRLVAAQEAAKTFLKELPRDVKVGIVAFAGSAQVAQQP
                     TLNREDLVTAIERFQMQRATATGNAIVISLATLFPHAGIELQQLSPDRMRGGWGRSLD
                     QPPPKQNEFVPVPPGSYSSAAIIMLTDGQRTTGVDPLDAAKMAAERGVKVYTVGIGTV
                     DGEIIGFEGWSMRVRLDEETLKTIAQKTAAEYFYAGTAQDLKKVYQSLSSRLTLEKKE
                     TEVSSLFALAGALLALLAGGLSLLWFGRVL"
     gene            complement(4497..5516)
                     /locus_tag="PKN51_07435"
     CDS             complement(4497..5516)
                     /locus_tag="PKN51_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT84969.1"
                     /translation="MLRWLRPRRPPPHAVPNPARADPQPGPAAADTEALLRRLEWTVL
                     RRLDGVLQGDVRTLMRGSGLDLADLREYQAHDDVRHIDWNVTARLQTPHVRVYTEDRE
                     MAAWFLLDLSPSVDFGSGQRLKREVLAEFVAVLARLLTRHGNRVGALLYGARVEQVIP
                     ARASRRHVLHLLHAIGHRPAPAPTAEAGTTRLAELLRAAGHLVHRRATLFVVSDFISA
                     PGWEQPLAQLASRHDVVAVRLLDPLERALPDLGLMTLRDAESGEQMLVDTHDAGFRQR
                     FAALAARHEDELRQTLARAGVDALELSTEGALTDAIVRFVELRRQRARLAAGEGLPAH
                     LRKAG"
     gene            complement(5551..6570)
                     /locus_tag="PKN51_07440"
     CDS             complement(5551..6570)
                     /locus_tag="PKN51_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT84970.1"
                     /translation="MTDKDNPRLETDPATAELMERILYEVKRVVVGQDRFLERVMVAL
                     LAGGHLLVEGVPGLAKTLTVKTLAAVLQGQFRRIQFTPDLVPADLIGTRIYNQKTGDF
                     STALGPVFTNLLLADEINRAPAKVQSALLEVMQERQVTIAGQTHFVPEPFLVMATQNP
                     IETEGTYPLPEAQVDRFMMKVLVDYPSDEEEFVIAQRVTGPRIEVQAVASMEQLAALQ
                     QQCRQVYVDPSLTQYAVRLVAATRRPADVGLKELAPMLGYGASPRATIGLIEGARALA
                     LMRGRAYALPEDMSDLVPDVLRHRLVLSYEALSEGLSADQIVLRLMAKVAPPAKPLAH
                     EPLAA"
     gene            complement(6596..7516)
                     /locus_tag="PKN51_07445"
     CDS             complement(6596..7516)
                     /locus_tag="PKN51_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT84971.1"
                     /translation="MRRSALYSRSSPAPVVAPDAPAPAPALAPERARRRPHLGAALRH
                     PGALWAAVLALALALALGLDLGAWRRPAPRQLTQKDIDDAVLHTATHKVLPSAAARAA
                     AAVAPSLVRVEGVVEQKKGRKGPPELGRGIGTGVVIIDNGTILTNLHVVQGAEQITVT
                     FADGTESPAGIVNMQPENDLAVLRAHKLPDDLQAATMRSTGDLAPGDEVVAIGYPFGI
                     GPSVSAGVVSGLGRGFRSPDGGQLMRNLIQFDAAANPGNSGGPLVTMDGQVVGIVTAI
                     LNPTPARTFLGIGFAVPIENAAAGAGQIPF"
     gene            7601..8002
                     /locus_tag="PKN51_07450"
     CDS             7601..8002
                     /locus_tag="PKN51_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT84972.1"
                     /translation="MGAMTVLRALLVWVLLLLLAIANGVLREAVLIPRLGGPSGLVLS
                     GLLLSALILVLAWWLLPWIGVRGHGPLLLTGLGWLALTLGFEFTFGLWRGRTLAELLA
                     AYRFEGGNLWSLVLLVTAGAPWLAGRLRRCT"
     gene            8030..8884
                     /locus_tag="PKN51_07455"
     CDS             8030..8884
                     /locus_tag="PKN51_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNT84973.1"
                     /translation="MAAWIDTHCHLDAPEFSADVAAVRARAATENVAICVIPAVEPAA
                     FEAVRALAHRGGDAYALGVHPMYVARAPEDALAQLAQALARHADDPRLVAVGEIGLDL
                     FVPELGVAPLRARQQHFFIEQLRLARRHDLPVLLHLRRAVDAVLQVLREVGVAGGIAH
                     AFSGSRQQADQLLALGFRLGFGGAVTYPRASRLRALAAELPASAIVMETDAPDIPPHW
                     LYTTAEARAQGQPQGRNEPGELPRIGAELAALRGVSAADWADLSTANALAALPRLRRL
                     WGAGEHDR"
     gene            9264..10718
                     /locus_tag="PKN51_07460"
     CDS             9264..10718
                     /locus_tag="PKN51_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT84974.1"
                     /translation="MTTTVDTPAGSPAATPTADAAPRRRGLGVTLTLLLIAALVGSAW
                     WLVQRARAPEAAAPAAAPGGAGGPGPGSASVTVGAAHAQRGELPIVIDALGTVTPPVT
                     ATLVPQVSGVLTEVLFTEGQMVSKGQVLARIDARPYEQALAQARGQRAKDEAQLAAAR
                     VTLARYQTLWQQDSIARQDVDTQAALVKQLEGVVEADRASERAAQLNVGFASIRAPIG
                     GRIGLRAIDPGNLVSTGSAGGIATITQIAPIDVVFSVPQDRIAAVLAAQRRGRLPVQA
                     FDRARSQPLAEGSFLTLDNQVSTATGTVRAKARFANADGALFPNQFVNARLQLGHDSG
                     VLVPVTAVRTGPQGDYVYVIDDERVARMRPVRRGMATVDQVLIVQGLQAGERVVTEGG
                     DRVKDGGKLRLAGESPGPGGAAGMRPGAAPAPAPTAPAAIASGAGEAGAAAPPWLERL
                     PPELRDKVRAMSPEERRAFLQQRRAAASAADSGR"
     gene            10745..13918
                     /locus_tag="PKN51_07465"
     CDS             10745..13918
                     /locus_tag="PKN51_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT84975.1"
                     /translation="MNVSRLFIQRPVATALCMLAIVLAGLLGLRLLPVAALPQVDYPT
                     IQVQTLYPGGSPDVMSRTVTAPLERQLGQMSGLERMSSVSSAGVSLITLQFALHLNMD
                     AAEQQVQAAMNAAGTLLPADLPAPPVYAKVNPADAPIMQLAVTSDSLPLPEVQNMVNT
                     RLALKISQIGGVGLVTLAGGQRPAVRVQANLQALAAMGLGLDAVSSAISAANTSSAKG
                     SFDGPTRQYTINANDQLLTAHEYRELIVAFVAGQPVRLKDVAEVVDGAENRRLGAWVA
                     IKNDSASRTPDARQSPGTSEIELPLTPAIVLNVQRQPGANVIATVDAIRRQLPELRQS
                     LPDSVRLTVLSDRTQGIRASVAHVELELGLAVVMVVLVIFAFLHSARATLIASIAVPI
                     SLAGTLAAMVLLGYSLNNLSLMALTIATGFVVDDAIVMIENISRHRELGKPPLQAALA
                     GAGEIGFTIISLTVSLIAVLIPLLFMQEVIGRLFREFAVTLAITILFSALVSLTLVPM
                     MSARWLEPLGQAHGPVGARMQAWLDATIAGYDRALRWVLARQPLTLALALLTLAVTVL
                     LYAGMPKGLFPTQSTGQLQGLLQAAPEVSFERMSALQGAAAQVVLRDPAVRSVSSVVG
                     VDAANNSALASGRLVINLRPRTPWGDTQATVMQRLRRQVGEQVAGVTLYLQPTQDLTI
                     DAVSGPTAQRFDLEGVDTALVDGWARRLVARLQRLPELRHVTTDAGATGLAAMVRVER
                     DTAARLGVTASNLDNALYNAFGQRIVSTIFSETNQYRVILEAARAVDASLRTLRELPV
                     RTSVGGTTPLEAFARVVEEPSPLQVQRVDQYPAATVGFDTAPGVSLGAAVSAIEAAVR
                     AEGLPAALTLRMTGAAGAFQQSLGNQLWLILAAVVCVYIVLGVLYESYVHPLTILSTL
                     PSAGVGALLALWLTGHPLDVVGIIGLVLLIGIVKKNAIMMIDFAIEAERAGKSALEAI
                     HQAALLRFRPILMTTLAALAAAVPLMLSWGDGGELRRPLGIAIFGGLVLSQLLTLFTT
                     PVIYLWFDRLGQRLAGRAPVGEVGHAD"
     gene            14070..17333
                     /locus_tag="PKN51_07470"
     CDS             14070..17333
                     /locus_tag="PKN51_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT84976.1"
                     /translation="MNLSRPFIHRPIATVLLTLGLALAGMAAYFVLPVARLPAVEFPV
                     IFVNASMAGASPADMARTVATPLERTLGTISGVNEMTSSSNTGSTRVVLQFDLGRDID
                     SAARDVQAAINAARIDLPASLRSNPTYRKANPAAQPFMTLALTSTTRTPGQIYDAVSN
                     IVSQRLQQVDGVGDVELGGGSLPAVRVQLNPFALHELGIGAEDVRAAIQAHNANRPKG
                     VIEVGGSADGRALQVLTPAPGLRAADYQDLIVALRDGHEVRLSQVARVIDSVQDTRTR
                     GMFNGQPAIVVNITQQPTANLIETADAIDALLPQLREQLPSDIELHVAVDRTGSVRAS
                     VREVEITLVIAVLLVVGVVGLFLRTLRAALIPAVATVVSLLGTLGVMYVLGFSLNNLT
                     LMALTVATGFVVDDAIVVLENITRHLEQGVARRRAALQGAREVGFTVLTISVSLVAVF
                     IPLLFMGGQVGRMFREFAVTLSAAVLISLLISLTTTPMLCALLLRPPAAGTPPRQSVA
                     GRLRQRLGGWARGSQRSLTRAYAGALDWALASPWLVVAVLLVVIGLNGYLFSAITKGF
                     FPDQDNGLMTGGLRADQSISSGAMADKLQQAVDILRADPSVGTVVAFSGGWRSGGGFL
                     SAALKPPGQRPPGESTRAVIQRLRPQLGRITGLRVFLNPVQELRIGGRSSNSSYQYTL
                     KTDNQADLRNWTRRLSEQMKRDARLTEIDDDQSEHSVETFVQIDRERAARLGVALPGI
                     DATLYDAFGQRQVATMFSALNQYAVVLEWAPEHARAPQALHDVMLPVAPAVSARLAGQ
                     AAPVASATSANPAQRGASTGQPISFTPAGMVPLPAIAEFSERAVPTSVWHDGGELSST
                     LSFDLADGVSIEQGRQAVLDAVDAVGLPNNVRGVFSGAAAQAQQTQAQQVLLIGAAIV
                     VIYIVLGILYESLIHPITVLTTLPSAGFGAVLALLLLKMEFSIMALIGVFLLIGIVKK
                     NAILIIDFALDAERARGLSATQAVREACLLRFRPILMTTLAAGLGALPLAIGFGQGSA
                     LRQPLGVTIVGGLIASQLLTLLTTPVVYVLLDRLRRRRPDQAHLARGVVAAPGADTT"
     gene            17330..18784
                     /locus_tag="PKN51_07475"
     CDS             17330..18784
                     /locus_tag="PKN51_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNT84977.1"
                     /translation="MNDSCFHSLRRLPDKRHSPVLSSVAAWVAAALLTACSLAPPERA
                     PELPLAGGWKGAAADGWVSTAAHQSWREGRWWTLFGDPDLDALMARLDIDNASLAQAV
                     AQVAQAEALLRQAEAQRAPVLGAQAGVQRSGRPARGSADLGLTASWAPDLWGRLGDAV
                     RAQGAQLQVSQADLAGARLAAQGSLAQAYFALRAADAEQALLDDIITGYERAATITGN
                     RYRAGIAAHTDLLQAQSTLDNARATRAGLQRNRASYEHAIAVLLGQPPARFTLAPAPW
                     IDQVPTVPPELPASLLLRRPDVAAAERAVAAANARIGVARAAWFPSLNLSASLGGGAS
                     GLSQLVSAPTLAWSLGAALAQTLFDAGARDAAIDQARAAHLAASAAYRQRALAAMGQV
                     EDQLSALATLATQIGHVSAAAAAAAAAEQRIMNSYQAGLSAYTNVVSAQASTLGARRA
                     LLQLQLQRQQAALALIEALGGGWQAPWAAAGGAP"
     gene            18833..>19095
                     /locus_tag="PKN51_07480"
     CDS             18833..>19095
                     /locus_tag="PKN51_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNT84978.1"
                     /translation="MPGPCTAPLDATTVAHLQQWCGRGETWHDQLHAAPLRGLSATLD
                     RDDPLPAPGSALPPLGHWLYFLPTARQSELGADGHPRRGGFLPP"
BASE COUNT         2579 a         6740 c         7023 g         2753 t
ORIGIN      
        1 cagctggtgg ccggcggcct gggcgaaggc cgccgccagc tcgccgtggc gtggcccgac
       61 cagcaccgcg ctggcctgtg catcggccag aatgtccagc tgttcgacca gggtgtggcg
      121 ggcattcagg gccacgtagg tgaggccggc cttttcggcg ccaaagccgc tgtcgaggct
      181 ctcgaccgag ttgtccagca acaccgccag ccgctcgccg ggttgcaggc ccagggccag
      241 cgccgcctgc gcgaaacggt tgctccgggc ctcgatctcg gcgtggctgt agcgttgttc
      301 accgaacacc accgcggtgc gcgcgccgtg ctgcgcggcg gcccaggtgg tgagtcgtcc
      361 ggcgatcatt ccagcaccac gaccgtctgg ccctcggcca cgctctcgcc ctcggccacc
      421 agcagctcca gcaccacacc ggcgcggggg gcggtgagcg ggatttccat cttcatgctc
      481 tccaggatga ccagggtctg gtcggcgctc accgggtcac cggcgctcac ctcgactttc
      541 cagacggtcc cggtgacttc ggacggcaga tggatgcgcg gcatgggatt ctcctgaatg
      601 tgggtgcccg cgatgataga caacatcaag attgttgaca atcgctatgt tgggtgattc
      661 aatcggcacc gacgttgccg ccttgcccct gtcctggcgc ggccacgccg tcccatcgaa
      721 gaggagatgt catgcaattt cgattcctgg ccggtctgat gctcggcggt ctgtgcctgg
      781 ccgccacggc cctggcccaa acggcatgga agcccagcga acgcatcaac tacatcattg
      841 gcgtggcacc gggcggcacg gtggatctgt atgcgcgtgc catcaaggac agcctggagc
      901 gcctgaaact gaccggcggc gaacccgtga tcgtggagaa caaggtgggt gccggcggcg
      961 ccctggcgat gaacgcgctg cgcgccggcc acggcaacgc ccattacctg ggcacgttcc
     1021 ataccggcgc catagccggc gcggtgaccg ggttgatcaa ggccgatccg cgtgaattca
     1081 tgccggtggc catgctggtg gaagagacct cggtggtggc ggtgcgcgcc gattcgccgt
     1141 tgcgcagcgg caccgatctg gtggccgcgc tcaaggccga cccgacggca ctgcgcatcg
     1201 cggtggcgcc ggcgctgggg cagaacatcc acatggcgat cgccaagccg ctgcatgtgg
     1261 cgggggtggc gatcgacaag ctgaccatcg cgccgtttcg ctcatccggt gagtccgtga
     1321 cggcgctgat cggcggtcac gtggacgtgg tgtcggccac tgcgccggtg gtgctgccgc
     1381 aggtggccaa cggcaagctg cgcatcctgg ccagcgcggc gcccgtgccg ggcagcgggc
     1441 cgctggcggg ggtgaagacc tggcgtgaac agggcgtgcc ggccgactac gtgagctaca
     1501 acggggtcac gctgccgcca ggcgtccatg ccgaacagat ccggttctgg gaggaggcgt
     1561 tgcgcaaggt ctcgcaggac ccgcagtggc gcgcgctggt cgaaaaatcg ggcaaccagc
     1621 cgatcttccg cggctatgtc gaatcccacc gctacatgat gagcgaatgg gacgcggccg
     1681 ccgaactggc ccgcacgctg ggcctgaagg gcggccgatg agcgcgcaac aaggccagac
     1741 gctggcgccg gccggggaat gggcggccga actggccgag ctgcagcagc gccgcgccag
     1801 cgcgctggcc atgggggggg cgcaggcgct ggcacgcatg cgcgacagcg gccgcatgaa
     1861 cgcgcgcgaa cgcatcgctg ccctgatcga ccagggcagt tttcacgaaa tgggcaccct
     1921 ggccggcaag ggccactatg acgcgcaagg gcgctacagc cataccgacc cgaccaacgc
     1981 catcgtcggc accgggcgca tcgacgggcg caaggtggcg ctgcacgtgg acgactacag
     2041 catccgtgcc ggcagctccg agggcaccat cgccgacaag tggatctaca tcgagcgcct
     2101 ggcgcaccag ctgcgcatgc cgctggtgcg gctggtcgat tcggccggcg gcagcgtcaa
     2161 gctgctgatg cagctgggcg gcaccaagat ccccgaatac accacctggc cggcgcagga
     2221 gctgctgcag accgtgccgg tggttggcgt ggcgctgggc gcctgcgccg ggcagggggc
     2281 ggtcaaggtg ctgtcttcgc atttttcggt gctggtgcgc gggcaggccc aggtgatggc
     2341 cgccgggccc catgtggtgc ggcaggccta cggggtcgag gtcgacaaga acgagctggg
     2401 tggccacctg gcgcagcgca agagcgccct ggtgcacaac gaggccgagg acgaggccga
     2461 cgcgctggcg caggtgcggc gctttctgtc ctatctgccg cgcagcgtgc atgagttggc
     2521 gccggtggtc gcctgcgacg acgatccgca ccgcgcggac gactggctca aggacgccgt
     2581 cccacgcgac cggcgcaaga tctacgaccc acgccgcatt ctggcggcgg tgttcgatca
     2641 gggcagcctg ttcgagatcg ggcgctggca gggcggctcg ctgctcaccc tgctggggcg
     2701 tctgaacggc gtgccggtgg gcgtgatcgc caacgacccc aaggtgcagg gcggcgccat
     2761 gacgctgcag gcggcctaca agatggaacg ccacacgcgg ctgtgcagcc agttcgggct
     2821 gccggtggtc aatttcgtcg atcagcccgg caacgccacc ggtctggagg ccgagctcgc
     2881 cggcaccctg ctgggtgcgg tgcggctggg cgaagccctg gcgcattgcc gctcaccctg
     2941 ggtgtcgatc ctgatgcgcc gctgcttcgg catggccggt gcgttgcatg ggcccaaggg
     3001 cggcgcgcgg ctgaatcacc gctttgcctg gccgtcggcg cgctggggct cgatcccgat
     3061 cgagggcggc gtgatggccg cgcacaaggc cgagatcgaa gccgcgcccg acccggccgc
     3121 caggcgcgcc gaactggagg cgcactatct ggccatgacc tcgccgtttc gcaccgccga
     3181 gaagttcggc gtgctcgaca tcatcgaccc gcgtgagacg cggcgcgtgc tgtgcgattg
     3241 ggtcgaggat gcgtggtacg tggtgcgagg tgacttggcg cagcgcagcg caggtgcatg
     3301 aggggcagcg atcgctatat aaacgatagc tgctggcgca tgttgggcgg gcgctgaggc
     3361 ccaaacggtc atgaaatccg tcagagcacg cggccgaacc acagcagcga caacccgccc
     3421 gccaacagcg ccagcagcgc gccggcgagg gcgaacagcg acgacacctc ggtttccttc
     3481 ttctccaggg tcaggcggct cgacaggctc tggtagacct tcttcaggtc ctgcgcggtg
     3541 ccggcgtaga agtactcggc ggcggtcttc tgggcgatgg tcttgagggt ttcctcgtcc
     3601 agccgcacgc gcatcgacca gccttcgaag ccgatgatct cgccatcgac cgtgccgatg
     3661 cccacggtgt agaccttgac gccgcgctcg gcggccatct tggcggcgtc cagcggatcg
     3721 acgccggtgg tgcgctggcc atcggtcagc atgatgatgg cggccgagct gtaggagccc
     3781 ggcggcaccg gcacgaactc gttctgcttg ggtggcggtt gatccagcga gcgaccccag
     3841 ccgccgcgca tgcggtcggg gctgagctgc tgcagctcga tgccggcgtg gggaaacagc
     3901 gtggccagcg aaatcacgat ggcgttgccg gtggcggtgg cgcgctgcat ctggaagcgc
     3961 tcgatggcgg tgaccaggtc ctcgcggttg agcgtgggct gctgcgccac ctgggccgag
     4021 ccggcaaagg ccacgatgcc taccttgacg tcgcgcggca gctccttcag gaaggtcttg
     4081 gctgcctcct gcgccgccac caggcggttg ggctcgacgt cggtggcgcg catgctggcc
     4141 gacacgtcca tcgccaggat gatggtctgc tggttggagg gcagggcgat caccgccacc
     4201 ggccgcgcgc tggccagcag cagggccgcc agcgccagca gcagcagcag tggcggcagg
     4261 tggcggcgcc agccggggcc gcggccctgc gcctgccgca cgatcgacag gctggcgtag
     4321 cgcaacgcca gcttcctgcg ccggcgcagc agccaggcgt acagcagcac cagcagcggc
     4381 agcagcgcca gcagccacag catgtgcggc cacaggaagg cgggctgcgg gatcgccgca
     4441 ggccacttca ggaaggcggg ccaggcgaag ttcatgagac cttcattttg atccgatcag
     4501 ccggccttgc gaaggtgcgc cggcagcccc tcgccggcgg ccaggcgggc gcgctggcgg
     4561 cgcagctcga cgaagcgcac gatggcgtcg gtcagcgcac cctcggtgga cagctccagc
     4621 gcgtcgacgc cggcgcgcgc cagcgtctgg cgcagctcgt cctcgtggcg ggccgccagg
     4681 gcggcaaagc gctgccgaaa gccggcgtcg tgcgtgtcca ccagcatctg ctcgccgctc
     4741 tcggcgtcgc gcagcgtcat caggccgagg tcgggcagcg cacgttcgag cggatcgagc
     4801 aggcgcaccg ccaccacgtc gtggcggctg gccagttgcg ccagcggctg ttcccagccg
     4861 ggcgcgctga tgaagtccga caccacgaac aaggtggcgc gccggtgcac caggtggccg
     4921 gccgcgcgca gcagctcggc caggcgcgtg gtccccgcct cggccgtcgg ggccggcgcc
     4981 ggccggtggc cgatggcgtg cagcagatgc agcacatggc ggcggctggc acgcgccggg
     5041 atcacctgct cgacccgcgc gccatagagc agggcgccga cgcggttgcc gtggcgcgtc
     5101 agcaggcgcg ccagcacggc gacgaattcg gccagcacct cgcgcttgag ccgctggccg
     5161 gaaccgaaat cgaccgaggg gctgaggtcc agcaggaacc aggccgccat ctcgcggtct
     5221 tcggtgtaga cgcgcacatg cggggtctgc aggcgcgccg tgacgttcca gtcgatgtgg
     5281 cgcacgtcgt cgtgggcctg gtattcgcgc aggtcggcca gatccagccc gctgccgcgc
     5341 atcagggtgc gcacgtcgcc ctgcagcacg ccgtccaggc gcctgagcac cgtccattcg
     5401 agccggcgca gcagggcctc ggtgtcggcc gcggccggcc cgggctgcgg gtcggcgcgc
     5461 gccgggttgg gcaccgcgtg cggcggtggc cggcgtgggc gcagccagcg cagcatcagc
     5521 ggcctcgtgg cgcagcgcgg tggtccatgc tcaggcggcc agcggctcgt gcgccagcgg
     5581 cttggccggc ggcgccacct tggccatcag gcgcagcacg atctggtcgg ccgacaagcc
     5641 ctccgacagc gcctcgtagg acagcaccag ccggtggcgc agcacgtccg gcaccaggtc
     5701 gctcatgtct tcgggcaggg cgtaggcgcg cccgcgcatc agcgccagcg ccctggcgcc
     5761 ttcgatcagg ccgatggtgg cgcgcgggct ggcgccgtag ccgagcatgg gcgccagctc
     5821 cttgagcccg acgtcggccg gccggcgcgt ggccgccacc aggcggacgg cgtactgcgt
     5881 gagcgagggg tcgacgtaca cctgccggca ttgctgctgc agggcggcca gctgctccat
     5941 gctggccacc gcctgcacct cgatgcgcgg gccggtcacg cgctgggcga tcacgaactc
     6001 ctcctcgtcg ctcgggtagt ccaccagcac cttcatcatg aagcggtcga cctgcgcctc
     6061 gggcagcggg taggtgccct cggtctcgat cgggttctgc gtcgccatca ccaggaacgg
     6121 ctcgggcacg aagtgggtct ggccggcaat ggtgacctgg cgctcctgca tcacctccag
     6181 cagcgcgctc tgcaccttgg ccggcgcgcg gttgatctcg tcggccagca gcaggttggt
     6241 gaacaccggg cccagcgcgg tcgagaagtc gcccgtcttc tggttgtaga tgcgggtgcc
     6301 gatcaggtcg gccggcacca ggtcgggcgt gaactggatg cgccggaatt ggccctgcag
     6361 cacggccgcc agcgtcttga cggtgagcgt cttggccagc cccggcacgc cctcgaccag
     6421 caggtggccg ccggccagca gggccaccat cacgcgctcc aggaagcggt cctggccgac
     6481 caccacgcgc ttgacctcgt agaggatgcg ctccatcagc tcggccgtgg ccgggtcggt
     6541 ctccaaacgc gggttgtcct tgtccgtcat gcgcggcatg ctcctgaaaa cggggttaga
     6601 agggaatctg cccggccccg gcggcggcgt tttcgatcgg caccgcaaag ccgatgccca
     6661 ggaaggtgcg cgccggcgtc gggttgagga tggcggtgac gatgcccacc acctggccgt
     6721 ccatggtcac cagcggcccg cccgagttgc ccgggttggc ggcggcgtcg aactggatca
     6781 ggttgcgcat caactggccg ccgtcgggcg agcgaaagcc gcgccccagg cccgacacca
     6841 cgccggccga caccgagggc ccgatgccga acgggtagcc gatcgccacc acctcgtcgc
     6901 cgggcgccag atcgccggtg gagcgcatgg tggcggcctg caggtcgtcg ggcagcttgt
     6961 gggcgcgcag caccgccagg tcgttttcgg gctgcatgtt gacgatgccg gccgggcttt
     7021 cggtgccgtc ggcaaaggtc acggtgatct gctcggcgcc ctgcaccacg tgcaggttgg
     7081 tcaggatggt gccgttgtcg atgatcacca cgccggtgcc gatgccacgg cccagttcgg
     7141 gcgggccttt gcggcccttc ttctgttcga ccacgccttc cacccgcacc agcgaaggtg
     7201 ccaccgcggc cgcagcgcgc gccgccgccg agggcagtac cttgtgcgtg gcggtgtgca
     7261 gcacggcgtc gtcgatgtcc ttttgcgtca gctggcgcgg cgccgggcgc cgccaggcgc
     7321 ccaggtcgag gcccagcgcc agcgccagcg ccagcgccag gaccgccgcc cacagcgcgc
     7381 cggggtggcg cagcgccgcc cccaggtgtg ggcgccggcg cgcacgctcg ggcgccagcg
     7441 ccggcgccgg cgccggggca tcgggcgcca ccactggggc aggggacgat cggctgtaca
     7501 gcgcgctgcg acgcatgggc tgaccccggg ctggtgaatg acgaagctca cggtaccatg
     7561 attcggcacg ccccggtagc ggggtggtca tggaggtggc atgggcgcga tgacggtgtt
     7621 gcgggcgctg ctggtgtggg tcctgctgct gttgctggcg atcgccaacg gcgtgctgcg
     7681 cgaggccgtg ctgatcccgc gcctgggcgg gccatccggc ctggtgctga gcggcctgct
     7741 gctgtcggcg ctgatcctgg tgctcgcctg gtggctgctg ccctggatcg gtgtgcgcgg
     7801 ccacgggccg ctgctgctga ccggcctggg ctggctggcg ctgaccctgg gcttcgagtt
     7861 cacctttggt ctgtggcgcg gccgcacgct ggccgaactg ctggccgcct atcggttcga
     7921 gggcggcaac ctgtggtcgc tggtgttgtt ggtgacggcc ggcgcgccgt ggctggccgg
     7981 ccggctgcgg cgctgtacct gacggtcgcg gctgcggcat gatgcgcgca tggccgcctg
     8041 gatcgacacc cactgccacc tggacgcccc ggagttttcg gctgatgtgg ccgccgtgcg
     8101 tgcgcgagca gctaccgaaa acgtagcgat ctgcgtgatc ccggcggtcg agccggcggc
     8161 cttcgaggcg gtgcgcgcgc tggcccaccg cggcggcgac gcctacgcac tgggcgtgca
     8221 cccgatgtac gtggcgcgcg ccccggagga cgcgctggcc cagctggcgc aggccctggc
     8281 gcgccatgcc gacgacccgc gcctggtggc ggtgggcgag atcgggctgg atctgttcgt
     8341 gcccgaactg ggcgtggcgc cgctgcgtgc gcgccagcag catttcttca tcgagcagct
     8401 gcggctggcg cggcgccacg acctgccggt gctgctgcac ctgcggcgcg cggtcgacgc
     8461 ggtgttgcag gtcctgcgcg aggtcggcgt ggccggcggc atcgcccatg ccttcagcgg
     8521 cagccgccag caggccgatc agttgctggc gctgggcttc aggctgggct ttggcggtgc
     8581 ggtgacctac ccgcgcgcca gccgcctgcg cgcgctggcg gccgagctgc cggccagcgc
     8641 catcgtgatg gaaaccgacg cgcccgacat cccgccgcac tggctctaca ccaccgccga
     8701 agcgcgcgcg cagggccagc cccagggccg caacgagccg ggcgagttgc cgcgcatcgg
     8761 cgccgagctg gcggcgctgc gtggcgtctc ggcggccgac tgggccgacc tcagcaccgc
     8821 caacgcgctg gcggcgctgc cgcgtctgag gcgactgtgg ggggccgggg agcacgatcg
     8881 atgacgatga cccatgaggc agggcgaagg acctcgctgc gcttggacgg cgggggcgcc
     8941 tcggggcatg ggggcgccgt gggcgctcgt tgaacgcgca gcgccgtcgg cctgcggggg
     9001 cgacgtggca cacccaccac gtcaaggcta tcgttttcat agctgcttgc gcagtctgga
     9061 caagcttcag aggcattttt tgcatggaat ccggcggcgc tggcgcgcgc tggcgcgcgc
     9121 tggcgcgggc gcggcggggt cggtggggct ttgcatatct tcacctggcg ctgacgggcg
     9181 gcccggggcc gggccgctac gatgcgcgct gaagcgccgg cgcctgtccc tgcgcgcgcg
     9241 cggcccgcca tgcattgccc accatgacca ccaccgtcga cacacccgca ggctcacccg
     9301 ccgccacccc gaccgccgac gccgcaccgc gccgccgcgg cctgggcgtc acgctcacgc
     9361 tgctgctgat cgccgcgctg gtgggcagcg cctggtggct ggtgcagcgc gcgcgcgcgc
     9421 ccgaggcggc ggcccccgcg gcagcgccgg gcggcgccgg tggcccgggg ccgggcagcg
     9481 ccagcgtcac cgtgggcgcg gcgcacgccc agcgcggcga actgccgatc gtcatcgacg
     9541 ccctgggcac ggtgacgccg ccggtcaccg ccaccctggt gccgcaggtg tcgggcgtgc
     9601 tgaccgaggt gctgttcacc gaggggcaga tggtgagcaa gggccaggtg ctggcgcgca
     9661 tcgacgcccg accctatgaa caggcgctgg cgcaggcgcg cggccagcgc gccaaggacg
     9721 aagcgcagct ggccgccgcc agggtgacgc tggcgcgcta ccagacgctg tggcagcagg
     9781 actcgatcgc gcgccaggac gtcgacaccc aggcggcgct ggtcaagcag ctcgaaggcg
     9841 tggtcgaggc cgaccgcgcc agcgagcgcg cggcccagct caatgtcggc tttgccagca
     9901 tccgcgcgcc gatcggcggg cgcatcggcc tgcgcgccat cgaccccggc aacctggtca
     9961 gcaccggcag cgccggcggc attgccacca tcacgcagat cgcgcctatc gacgtggtgt
    10021 tctcggtgcc gcaggaccgc atcgccgccg tgctggcggc ccagcggcgc gggcgcctgc
    10081 cggtgcaggc gttcgaccgc gcgcgcagcc agccgctggc cgaaggcagc tttctgacgc
    10141 tggacaacca ggtcagcacc gccaccggta cggtgcgtgc caaggcccgc tttgccaacg
    10201 ccgacggcgc gctgtttccc aaccagttcg tcaacgcccg gctgcagctg gggcacgaca
    10261 gcggcgtgct ggtgccggtg acggcggtgc gcaccggccc gcagggtgac tacgtgtacg
    10321 tgatcgacga cgagcgggtg gcgcgcatgc gcccggtcag gcgcggcatg gcgacggtcg
    10381 atcaggtgct gatcgtccag ggcctgcagg ccggcgagcg cgtggtgacc gaaggcggcg
    10441 accgcgtcaa ggacggcggc aagctgcgcc tggcgggcga atcgccgggc ccgggcggcg
    10501 ccgccggcat gcgccccggt gcggcgccag cgcccgcccc gacggcgccg gcagctatcg
    10561 catcaggagc aggtgaggcc ggcgcggctg ccccgccgtg gctggagcgc ctgccgccgg
    10621 agctgcgcga caaggtgcgc gccatgtcgc ccgaggagcg ccgggccttc ctgcagcagc
    10681 gccgcgcggc ggccagcgcg gccgattcgg gccgctgagg cggcggcagc accggcccct
    10741 cgtcatgaac gtctcgcgcc tgttcatcca gcgcccggtg gcgacggcgc tgtgcatgct
    10801 ggccatcgtg ctggccggcc tgctgggcct gcggctgctg ccggtggcgg cgctgccgca
    10861 ggtcgactac ccgaccatcc aggtgcagac gctctacccc ggcggcagcc ccgacgtgat
    10921 gagccgcacc gtgacggcgc cgctggagcg tcagctgggc cagatgtccg ggctggaacg
    10981 catgagcagc gtcagcagcg ccggcgtgtc gctgatcacg ctgcagttcg cgctgcacct
    11041 gaacatggac gccgccgagc agcaggtgca ggccgccatg aacgccgccg gcaccctgct
    11101 gccggccgac ctgccggcgc cgccggtgta cgccaaggtc aacccggccg atgcgcccat
    11161 catgcagctg gccgtcacct ccgacagcct gccgctgccc gaggtgcaga acatggtcaa
    11221 cacccggctg gcgctgaaga tcagccagat cggtggcgtc ggcctggtga cgctggccgg
    11281 cggtcagcgt cccgccgtgc gggtgcaggc caacctgcag gcgctggccg ccatgggcct
    11341 ggggctggac gccgtcagca gcgccatcag cgccgccaac accagctcgg ccaagggcag
    11401 cttcgacggc ccgacgcgcc agtacaccat caacgccaac gaccagctgc tgacggcgca
    11461 cgaataccgc gagctgatcg tcgccttcgt ggccggccag ccggtgcgcc tgaaggacgt
    11521 cgccgaggtg gtggacggcg ccgaaaaccg tcgcctgggg gcctgggtgg ctatcaaaaa
    11581 cgatagcgcc tcgcgcacgc cggatgcgcg ccagagcccc ggaacatctg aaatcgagct
    11641 gccgctcacg ccggccatcg tgctcaacgt gcagcgccag ccgggcgcca acgtgatcgc
    11701 caccgtcgac gccatccggc gccagctgcc cgagctgcgg caaagcctgc ccgacagcgt
    11761 gcggctgacc gtgctgagcg accgcacgca gggcatccgc gcctcggtgg cgcacgtgga
    11821 gctggagctg ggcctggcgg tggtgatggt ggtgctggtg atctttgcct ttctgcacag
    11881 cgcgcgcgcc acgctgatcg ccagcatcgc ggtgccgatc tcgctggccg gcacgctggc
    11941 ggccatggtg ctgctgggct attcgctcaa caacctgagc ctgatggcgt tgaccatcgc
    12001 caccggcttc gtggtggacg acgccatcgt gatgatcgag aacatctcgc gccaccgcga
    12061 gctgggcaag ccgccgctgc aggcggcgct ggcgggggcg ggcgagatcg gcttcaccat
    12121 catctcgctg acggtgtcgc tgatcgcggt gctgatcccg ctgctgttca tgcaggaggt
    12181 gatcggccgg ctgttccgtg aattcgccgt caccctggcc atcaccatcc tgttttccgc
    12241 gctggtgtcg ctgaccctgg tgcccatgat gtcggcgcgc tggctggagc cgctgggtca
    12301 ggcccatggc ccggtgggcg cccgcatgca ggcgtggctg gacgccacca tcgccggcta
    12361 cgaccgcgcg ctgcgctggg tgctggcgcg tcagccgctg acgctggcgc tggcgctgct
    12421 gacgctggcc gtcaccgtgc tgctgtacgc gggcatgccc aaggggctgt tcccgaccca
    12481 gagcaccggt cagctgcagg gcctgctgca ggcggcgccg gaggtgtcgt tcgagcgcat
    12541 gtcggcgctg cagggtgcgg cggcgcaggt ggtgctgcgc gatccggcgg tgcgctcggt
    12601 cagttcggtg gtgggggtgg acgcggccaa caacagcgcg ctggcgagcg ggcggctggt
    12661 catcaacctg cggccgcgca cgccctgggg cgacacgcag gcgaccgtca tgcagcggct
    12721 gcgccggcag gtgggcgagc aggtggccgg cgtcaccctg tacctgcagc cgacgcagga
    12781 cctgaccatc gacgccgtca gcggcccgac ggcgcagcgc ttcgacctgg agggggtgga
    12841 cacggcgctg gtcgatggct gggcgcggcg cctggtggcg cgcctgcagc gcctgcccga
    12901 gctgcgccac gtcaccaccg acgccggcgc caccggcctg gccgccatgg tgcgggtcga
    12961 gcgcgacacc gccgcacgcc tgggcgtgac agccagcaac ctggacaacg cgctctacaa
    13021 cgcctttggc cagcgcatcg tctccaccat cttcagcgaa accaaccagt accgcgtgat
    13081 cctggaggcc gcgcgcgccg tggacgccag cctgcgcacc ctgcgcgagc tgccggtgcg
    13141 caccagcgtc ggcggcacca cgccgctgga agcctttgcg cgcgtggtgg aagagccgtc
    13201 gccgctgcag gtgcagcggg tcgaccagta ccccgccgcc accgtgggct tcgacaccgc
    13261 gccgggcgtc agcctggggg cggcggtgag cgccatcgag gcggcggtgc gcgccgaggg
    13321 cctgccggcg gcgctgacgc tgcgcatgac cggtgccgcc ggcgccttcc agcagtcgct
    13381 gggcaaccag ctgtggctga tcctggcggc ggtggtgtgc gtctacatcg tgctgggcgt
    13441 gctgtacgag agctacgtgc acccgctgac gattctgtcg acgctgccct cggccggcgt
    13501 gggcgcgctg ctggcgctgt ggctgaccgg ccatccgctg gacgtggtgg gcatcatcgg
    13561 cctggtgctg ctgatcggca tcgtgaagaa gaacgcgatc atgatgatcg acttcgccat
    13621 cgaggccgaa cgcgccggca agtcggccct ggaggcgatt caccaggcgg cgctgctgcg
    13681 tttccggccg atcctgatga ccaccctggc ggcgctggcc gccgccgtgc cgctgatgct
    13741 gagctggggc gacggcggcg agctgcgccg gccgctgggc atcgccatct ttggcggtct
    13801 ggtgctgtcg cagctgctga cgctgttcac cacgccggtg atctacctgt ggttcgaccg
    13861 cctgggccag cggctggccg gtcgcgcccc ggtgggggag gtggggcatg ccgactgagg
    13921 cgctgcacgg cgggccaagc gctactgctt tggtagctgg tggcgcttgc tgggcgggcg
    13981 ttgcgggctt tttttgtcgc ttgtggctgg cgccctcgac cgtctgggga gaggcccggg
    14041 cccgcatgcc cccggcgggg cgctgagcca tgaacctctc gcgccccttc atccaccggc
    14101 ccatcgccac cgtgctgctc accctcgggc tggcgctggc cggcatggcg gcgtacttcg
    14161 tgctgccggt ggcgcggttg ccggcggtgg agtttccggt catcttcgtc aacgccagca
    14221 tggccggcgc cagcccggcc gacatggcgc gcaccgtcgc cacgccgctg gaacgcaccc
    14281 tgggcaccat ctcgggcgtc aatgaaatga cctccagcag caacaccggc tcgacccgcg
    14341 tggtgctgca gttcgatctg gggcgcgaca tcgacagcgc cgcgcgcgac gtgcaggcgg
    14401 ccatcaacgc cgcccgcatc gacctgccgg ccagcctgcg cagcaacccg acctaccgca
    14461 aggccaatcc ggcggcgcag cctttcatga cgctggcgct gacttcgacc acgcgcacgc
    14521 cggggcaaat ctacgacgcc gtcagcaaca tcgtcagcca gcgcctgcag caggtcgatg
    14581 gcgtgggcga cgtggagctg ggcggcggct cgctgccggc ggtgcgggtg cagctcaacc
    14641 ccttcgcgct gcacgagctg ggcatcggcg ccgaggacgt gcgcgccgcc atccaggcgc
    14701 acaacgccaa ccgccccaag ggcgtgatcg aagtcggcgg cagcgccgac gggcgcgcgc
    14761 tgcaggtgct gacccccgcg cccggcctgc gcgcggccga ttaccaggac ctgatcgtgg
    14821 ccctgcgcga cggccacgag gtgcgcctgt cgcaggtggc gcgcgtgatc gacagcgtgc
    14881 aggacacgcg cacgcgcggc atgttcaacg gccagccggc gatcgtggtc aacatcacgc
    14941 agcagcccac cgccaacctg atcgagaccg ccgacgccat cgacgcgctg ctgccgcagc
    15001 tgcgcgagca gctgcccagc gacatcgagc tgcacgtggc ggtcgaccgc accggctcgg
    15061 tgcgcgcctc ggtgcgcgaa gtcgagatca ccctggtgat cgcggtgctg ctggtggtgg
    15121 gtgtggtggg gctgttcctg cgcacgctgc gggcggcgct gattccggcg gtggccacgg
    15181 tggtgtcgct gctgggcacg ctgggtgtga tgtacgtgct gggcttcagc ctgaacaacc
    15241 tgaccctgat ggcgctgacg gtggccaccg gcttcgtggt ggacgacgcc atcgtggtgc
    15301 tggaaaacat cacccgccac ctggagcagg gcgtggcgcg gcgccgcgcg gcgctgcagg
    15361 gcgcgcgcga ggtcggcttc acggtgctga ccatcagcgt gtcgctggtg gcggtgttca
    15421 ttccgctgct gttcatgggc gggcaggtgg ggcgcatgtt ccgcgagttc gccgtcacgc
    15481 tgtcggcggc ggtgctgatt tcgctgctga tctcgctcac caccacgccc atgctgtgcg
    15541 cgctgctgct gcgcccgccg gcggcgggca cgccaccgcg ccagagcgtc gccggccggc
    15601 tgcgccagcg cctgggcggc tgggcgcgcg gcagccagcg gtcgctgacg cgcgcctacg
    15661 ccggcgcgct ggactgggcg ctggcctcgc cctggctggt ggtggcggtg ctgctggtgg
    15721 tgatcgggct gaacggctac ctgttctcgg ccatcaccaa gggcttcttc ccggatcaag
    15781 acaacggcct gatgaccggc gggctgcgtg ccgaccagag catctcctcc ggcgccatgg
    15841 ccgacaagct gcagcaggcg gtcgacatcc tgcgcgccga tccgtcggtg ggcacggtgg
    15901 tggcgttttc gggcggctgg cgctcgggcg gcggctttct gtcggcggcg ctcaaaccac
    15961 ccggccagcg gccgccgggc gagagcacgc gcgccgtcat ccaacggctg cgcccgcagc
    16021 tggggcgcat caccgggctg cgcgtgtttc tcaacccggt gcaggagctg cgcatcggcg
    16081 ggcgcagcag caacagcagc taccagtaca cgctcaagac cgacaaccag gccgacctgc
    16141 gaaactggac gcgccgcctg tccgagcaga tgaagcgcga cgcccgcctg accgagatcg
    16201 acgacgacca gagcgagcac agcgtggaga ccttcgtgca gatcgaccgt gagcgcgccg
    16261 cccgcctggg cgtggcgctg ccgggcatcg acgccacgct gtacgacgcc ttcggccagc
    16321 gccaggtggc cactatgttc agcgcgctca accagtacgc ggtggtgctg gaatgggcgc
    16381 cggagcatgc gcgcgcgccg caggcgctgc acgacgtgat gctgccggtg gcgccggcgg
    16441 tgtcggcgcg gctggcgggc caggcggcgc cggtggccag cgccacctcg gccaacccgg
    16501 cgcagcgcgg ggcctccaca gggcagccga tttcgttcac gccggccggc atggtgccgc
    16561 tgccggcgat cgccgagttc agcgagcgcg ccgtgcccac cagcgtctgg cacgacggtg
    16621 gcgagctgtc cagcacgctg tcgttcgacc tcgccgatgg ggtgtcgatc gagcagggcc
    16681 gccaggcggt gctggacgcg gtcgatgccg tcggcctgcc caacaacgtg cgcggcgtct
    16741 tcagcggcgc ggcggcgcag gcgcagcaga cgcaggcgca gcaggtgctg ctgatcggcg
    16801 ccgccatcgt ggtgatctac atcgtgctgg gcatcctgta cgaaagcctg atccacccca
    16861 tcaccgtgct gaccacgctg cccagcgccg gcttcggcgc cgtgctggcg ctgctgctgc
    16921 tgaagatgga gttttccatc atggcgctga tcggcgtgtt cctgctgatc ggcatcgtga
    16981 agaagaacgc catcctgatc atcgacttcg cgctcgacgc cgagcgcgcg cgcggcctga
    17041 gcgccacgca ggcggtgcgc gaagcctgcc tgctgcgctt tcgcccgatc ctgatgacca
    17101 ccctggccgc cggcctgggc gcgctgccgc tggcgatcgg cttcggccag ggctcggcgc
    17161 tgcgccagcc gctgggcgtg accatcgtcg gcggcctgat cgccagccag ttgctgaccc
    17221 tgctgaccac gccggtggtc tatgtgctgc tcgaccgact gcgccgccgg cgccccgatc
    17281 aggcccatct ggcgcgcggc gtggtcgccg cgccaggggc ggacacgaca tgaacgactc
    17341 ctgttttcat agcttgcggc gcttgccaga caagcgccat agccctgttt tgtcttcggt
    17401 tgccgcatgg gtggcggccg cgctgttgac cgcttgcagc ctggcgccgc ccgagcgcgc
    17461 gcccgagctg ccgctggccg gcggctggaa gggcgccgcc gccgacggat gggtcagcac
    17521 cgccgcgcac cagtcctggc gcgaagggcg ctggtggacg ctgttcggcg accccgatct
    17581 cgacgcactg atggcgcgcc tggacatcga caacgccagc ctggcgcagg ccgtggccca
    17641 ggtggcgcag gccgaggcgc tgctgcgcca ggccgaggcg cagcgcgccc cggtgctggg
    17701 cgcgcaggcc ggggtgcagc gcagcggccg gccggcgcgc ggttcggccg acctgggcct
    17761 caccgccagc tgggcgcccg acctgtgggg ccggctgggc gacgccgtgc gcgcccaggg
    17821 cgcccagctg caggtcagcc aggccgatct ggccggcgcg cggctggcgg cccagggcag
    17881 cctggcgcag gcctacttcg ccctgcgcgc ggccgacgcc gaacaggcgc tgctggacga
    17941 catcatcacc ggctacgaac gcgccgccac catcaccggc aaccgctatc gcgccggcat
    18001 tgccgcccac accgacctgc tgcaggccca gagcacgctg gacaacgccc gtgccacgcg
    18061 cgccggcctg cagcgcaacc gcgccagcta cgagcacgcc attgccgtgc tgctgggcca
    18121 gccgccggcc cgcttcacgc tcgcgcccgc gccctggatc gaccaggtgc ccacggtgcc
    18181 gccggagctg ccggccagcc tgctgctgcg ccgccccgac gtggcggcgg ccgagcgcgc
    18241 ggttgccgcg gccaacgcgc gcatcggcgt ggcgcgggcg gcctggttcc ccagcctcaa
    18301 cctcagcgcc tcgctcggtg gcggcgccag cgggctgtcg caactggtgt cggcgcccac
    18361 cctggcctgg tcgctcggcg cggcgctggc gcagaccctg ttcgacgccg gcgcgcgcga
    18421 cgccgccatc gaccaggcgc gggccgcgca cctggcggcc agcgccgcct accgtcagcg
    18481 cgccctggcc gccatgggcc aggtggaaga ccagctcagc gcactggcca cgctggcgac
    18541 gcagatcggc cacgtgagcg ccgccgccgc cgcggcggcc gccgccgagc agcgcatcat
    18601 gaacagctac caggccggct tgtcggccta caccaacgtc gtcagcgccc aggccagcac
    18661 cctgggtgcg cgccgtgcgc tgctgcagct gcaactgcag cgccagcagg cggcgctggc
    18721 gctgatcgag gccctgggtg gcggctggca ggcgccctgg gcagcggccg gtggcgcgcc
    18781 atgacccccg ccggcgcttg ccgcgggcgc gtcgccacgc catgatggac gcatgcccgg
    18841 cccttgcacc gcccctcttg acgccaccac cgtcgcccac ctgcagcagt ggtgcggccg
    18901 cggcgagacc tggcacgatc agctgcacgc ggcgccgctg cgcggcctgt cggccacgct
    18961 ggaccgtgac gacccgctgc ccgcaccggg cagtgcgctg ccgccgctgg ggcactggct
    19021 gtactttctg cccaccgccc gccagagcga gctgggcgcc gacggccatc ccaggcgcgg
    19081 cggctttctg ccgcc
//
LOCUS       DAOQTZ010000049        19061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3345_length_19061_cov_12.194833, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000049 DAOQTZ010000000
VERSION     DAOQTZ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 19061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19061
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3345_length_19061_cov_12.194833"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PKN51_07485"
     CDS             complement(<1..913)
                     /locus_tag="PKN51_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT84979.1"
                     /translation="MTSPRFGAPWPSAALQPALIDAGPAQPAAAPAGATQPLVQAEAL
                     ARTFDVSPPLLNRLLEGKPRQLLHAVDEASFSIQAGQTLALVGESGCGKSTVARVLVG
                     LYQPTRGRFLFKGQDAHAAFRGKDGHHLRRSIQMIFQDPYASLNPRWKVADIIAEPLH
                     EHGMAASRAQALERVGELLQSVGLAAADGVKYPHQFSGGQRQRISIARALATQPEFLV
                     CDEPTSALDVSVQAQVLNIMKELQRQRGLTYLFISHNLAVVRHVSDAVGVMYLGRIVE
                     LAPKAELFSAPRHPYTRMLLDAIPKMHD"
     gene            complement(910..1884)
                     /locus_tag="PKN51_07490"
     CDS             complement(910..1884)
                     /locus_tag="PKN51_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT84980.1"
                     /translation="MSLLEVRNLIVEFPNRRGTLRALDDVSFEIAPGEILGVVGESGA
                     GKSLTGASIIGLLEPPGRVASGQIVLEGQRIDQLSHEQMRHIRGRKIGAIFQDPLTSL
                     NPLYSVGRQLVETIQTHLPVSAAEARQRAIDLLKDTGIPAAEQRIDHYPHQFSGGMRQ
                     RVVIALALAAEPKLIVADEPTTALDVSIQAQIIGLLKRVCRERGAAVMLITHDMGVIA
                     ETCDRVAVMYAGRVAEIGPVHEVINHPAHPYTAGLMASIPDMEQDRETLNQIDGAMPR
                     LNAIPRGCAFNPRCPRVFERCRVERPDLLPAGATQAACWLHARQEVAA"
     gene            complement(2016..2927)
                     /locus_tag="PKN51_07495"
     CDS             complement(2016..2927)
                     /locus_tag="PKN51_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT84981.1"
                     /translation="MSNALARWWDSDVAYSFRSSPTAIVAALVGLVCVFCAVFAPWVA
                     PHNPLDLTTLELSDARLPPFWMEGGSSKYWLGTDDQGRDILSALMYGARISLVVGLAS
                     VLLSVLVGVGLGLIAGFRGGWVDGFLMRVCDVMLSFPAILIALLVAGVARAKFPDAGT
                     SVAFGVLIIAIALTGWVQYARTVRGSTMVERGKEYVQAARVTGVAPWRIMLKHVLPNV
                     MGPVMVLATIQVATAIITEATLSFLGVGVPPTSPSLGTLIRVGNDYLFSGEWWITIFP
                     GVMLVLIALSVNLLGDWLRDALNPRLR"
     gene            complement(3018..3998)
                     /locus_tag="PKN51_07500"
     CDS             complement(3018..3998)
                     /locus_tag="PKN51_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT84982.1"
                     /translation="MGAFILRRLLQAIVVMVSVGFIAFLLFQYVGDPVLILLGQDATP
                     DQIRQLRADLGLDQPFFVQFWHFLLNAVQGEFGLSLRQGAKVSRLLGERFPATFELAL
                     VAAALALLIGIPMGVYAALRRGSFLSQVFMTVSLLGVSLPTFLIGILLILIFSVWLGW
                     LPSFGRGETVRIGWWSSGLFTLDGWLHMILPALTLAIFQLTLIMRLVRAEMLEVLRTD
                     YIKFARARGLTDRAIHFGHALKNTLVPVMTITGLQLGGLIAFAIITETVFQWPGMGLL
                     FIQAVTFADVPVMAAYLCLIALIFVVINLVVDLLYVVVDPRLRVAKAAGH"
     gene            complement(4193..5101)
                     /locus_tag="PKN51_07505"
     CDS             complement(4193..5101)
                     /locus_tag="PKN51_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNT84983.1"
                     /translation="MKKSLIALAVLGFAGAAMAQSSVTLYGVADAGLGKTKSAGESRV
                     NMHSANTMNNGNSRLGVRGVEDLGGGLKAGFNFETGLSLEDGSTAASGPGFWSRNANM
                     WVGGNWGTFKMGRTLNPSFYGVAAWELTGTANYSVVGNTYGYVGGARNSSQFSYKTPD
                     MGGLSAELGYIFKDDNAGAAKWDLNVVYANGPIAAGLSANKAKGQKVGYAIGGKYNFG
                     NFAVAASYSDNHKVRRGFSIGGTATFGAASVTLDLTRDTKHPVKKYTNGLLEGKYALS
                     KRTFLYAAYLRLDGENNYGLGVRHNF"
     gene            complement(5506..6435)
                     /locus_tag="PKN51_07510"
     CDS             complement(5506..6435)
                     /locus_tag="PKN51_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNT84984.1"
                     /translation="MKKSLIALAVVALSGAAMAQSSVTLYGVADAGIGKIKSGGNDSS
                     NKAQFTSASTMNNGDSRLGVRGVEDLGAGLKIGFNFETGLDLDDGATTTSSFWARQAN
                     LSVGGNWGTVKLGRQFTPSYLTTATYEVTGTANYSALGKTYRYAGIGSRANSAFAYIT
                     PNFGGFTAAAAYVSKHDVGANVWDLGLMYNNGPIAAGLSANKVSGGKTNYQLGGKYSF
                     GNFALAASYNQASDAGGLRRRGASIGGSATFGATSIALDLTRDTKNEWTGKKYTNGVL
                     EAKYALSKRTFVYGAFLRYDGDNNYGVGVRHNF"
     gene            6966..7601
                     /gene="coq7"
                     /locus_tag="PKN51_07515"
     CDS             6966..7601
                     /gene="coq7"
                     /locus_tag="PKN51_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNT84985.1"
                     /translation="MPTAIDRLLAAADSGLRTLLAPPRAARPSPAAAMTASTPLTEDE
                     QRLSGALMRVNHVGEICAQALYTAQALGTSDPALRRQLDAAAREETDHLAWTRERLDQ
                     LGDRVSLLNPLWFAGAFGIGLLAGRIGGDRLSLGFVVETERQVEAHLASHLDRLPAAD
                     SASRAIVAQMKDDEARHADAALHAGGVALPAPVRGLMRLAAKVMTVTAHRI"
     gene            complement(7847..8296)
                     /locus_tag="PKN51_07520"
     CDS             complement(7847..8296)
                     /locus_tag="PKN51_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNT84986.1"
                     /translation="MECTVSWTGAAGTRSGMGFVAETGSGHVIAMDGAPDAARPEFGG
                     QNLAPRPMETLLAGTGGCTAYDVVLILRRGRHDVRGCSVRLSSERASEDPKVFTAIHM
                     HFTVSGRGVPAAAVERAIQLSHDKYCSASIMLGKTATITTSFEVVEV"
     gene            8632..10203
                     /gene="ilvA"
                     /locus_tag="PKN51_07525"
     CDS             8632..10203
                     /gene="ilvA"
                     /locus_tag="PKN51_07525"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNT84987.1"
                     /translation="MTPPASAPTGAAALSPTDYLKQVLTARVYDVAVESDLDPARSLS
                     RRLHNKVTLKREDQQPVFSFKLRGAYNKMAHLTPEQLHKGVICASAGNHAQGVALGAH
                     KLGTRAVIVMPTTTPQVKVDAVRALGGEVVLAGDSYSDAYEHALGLQAEQGLTFIHPF
                     DDPLVIAGQGTVGLEILRQHKGPLHAVFVPIGGGGLISGVGACIKAVRPEVKVIGVQM
                     SDSDAMAQSVRQGRHVQLADVGLFADGTAVKRVGDETLRLAGAVVDEFITVDTDAVCA
                     AIKDVFIDTRSIVEPSGAMAVAALKQYVATHKTRGESYVALLSGANMNFDRLRFVAER
                     AEVGEEREALFAVTIPEARGSFRRFLEVIGSLPGGARNVTEFNYRISDAAQAHVFIGL
                     TTAARGESPKIAAHFERHGLKTVDLTHDELAKEHVRHMVGGRSSLAQHERLLRFVFPE
                     RPGALLKFLSLMQPSWNISLFHYRNQGADYGRILVGMQVPASDDAAFRRFLAELGYPW
                     VEETDNPAYRLFLQR"
     gene            complement(10375..11016)
                     /locus_tag="PKN51_07530"
     CDS             complement(10375..11016)
                     /locus_tag="PKN51_07530"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNT84988.1"
                     /translation="MTQRLASRLAVGLPTLLLWALLAGSALWWWLRAGQPAAPQPAPV
                     AGGAPAARTVDSAQVARVLGATAAAPAAPAAAPELGGRVVLKGVVTHGARGAALIALD
                     GQPARPLRVGAELEGVEGGWRLRSVAPHGALLAAAGGRELRLEMPPLERRSSGSAAAV
                     PSHPAPPVPAGRPGTSRAGPMAPVVPTPPTLPAGMAVSPQPQAFPAPGAAPAQ"
     gene            11215..11658
                     /gene="gspG"
                     /locus_tag="PKN51_07535"
     CDS             11215..11658
                     /gene="gspG"
                     /locus_tag="PKN51_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNT84989.1"
                     /translation="MTLSPPIAARARRAAGFTLIELMVVLVIIGVLAALIVPNVLDRT
                     DDARATAARTDVHNLMQALKLYKLDNQRYPTAEQGLAALVQRPSAPPAPGNWKPYLEK
                     LPNDPWGQPYQYLNPGIRGEVDVMSFGADGKSGGEGKDADIGSWQ"
     gene            11798..12232
                     /locus_tag="PKN51_07540"
     CDS             11798..12232
                     /locus_tag="PKN51_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT84990.1"
                     /translation="MKSRRGFTLIELMVVIAIVAIGSALASLALPDSDRRTLAREGER
                     LAALLEAARAQSRAAGVPVRWRATASGFAWDGLPPGAAPLPTGWLDARTRALGNAALL
                     LGPDPIIDAQAVVLTRPDQHSPQALRIASDGLRPFGVAPLTP"
     gene            12229..12606
                     /gene="gspI"
                     /locus_tag="PKN51_07545"
     CDS             12229..12606
                     /gene="gspI"
                     /locus_tag="PKN51_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HNT84991.1"
                     /translation="MSRRRAAAPPDAGFTLVEVLVALAITAIALIAGFKASAALTDNA
                     GRQGTVLLAQICAENRLTELRLARQLPAVGDSELACAQAERQFLVTQAVRPTPNPNFR
                     RVEAAVSEAGVPVLRLSTIVGRH"
     gene            12699..13397
                     /locus_tag="PKN51_07550"
     CDS             12699..13397
                     /locus_tag="PKN51_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT84992.1"
                     /translation="MRGPRGFTLIELLVALSLVAVIAVLSWRGIDAMARAQQSTQQHT
                     DEVLALQAGLAQWRADLDALMSWPAAPAAPGQPPASGPASQRSLAWDGRTLRLTRSDA
                     GDAAAGLRVVAWTRRPASGQWQRWQSPPLASQAAWAAAWDAAAHWGQSDTEPPAAAGA
                     SAVTLARALDWQLHYYRHNAWTHPLSSSAESGSATQLLPDGVRLFLTLAAGQALAGPL
                     VLDWVRPDFSGAQP"
     gene            13610..14572
                     /gene="gspK"
                     /locus_tag="PKN51_07555"
                     /pseudo
     CDS             13610..14572
                     /gene="gspK"
                     /locus_tag="PKN51_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702531.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
     gene            14601..15818
                     /gene="gspL"
                     /locus_tag="PKN51_07560"
     CDS             14601..15818
                     /gene="gspL"
                     /locus_tag="PKN51_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HNT84993.1"
                     /translation="MSLLLIALPPGPPADYAFATSADGQGVSAHGSAAPALLPAAGRG
                     VEVVAVVPAARLSWQAVQLPRGVGPGAPRLRSILEGLLEEQLLDEPARLHLALAPGAQ
                     GGARAWVAVCERAWLAAHLAALDAAGRPPARIVPELSPQHGPTRLWLSGEPERPWLLM
                     SGDGVPGGAQALPFGPGSAALLPAAAADAPAPELLAEPALAALAEQAFQRPVAIRSAA
                     ERLLHASRSPWDLAQLEFSRGGRARAARRAGTLWRDFLHAPAWRPARWALALLLLVNL
                     AGLNLLAWRTGQALAAQRQAQQVLLTDTFPQVKVVVDAPVQMAREVAALRQATGAASG
                     RDLEPMLQAFGQLALTQQAPTAIEFEAGELRLKGLTLAAGALAEANPRLRPLGLHLRT
                     EGDTAVLRQEGAP"
     gene            15877..16401
                     /gene="gspM"
                     /locus_tag="PKN51_07565"
     CDS             15877..16401
                     /gene="gspM"
                     /locus_tag="PKN51_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNT84994.1"
                     /translation="MSAPASAAAPTRGPRALGAAWARLAARERRLVLLAAAVLGLALL
                     WLLAVAPALRTLRQAEAQRATLAAQTEQLQRLRAEAQALQALPRLGHDEALRALESSV
                     RQRLGDSAQLSVVGERATLVLRQTSAEALAQWLADARANARANTVEARLTRAGDNAPG
                     APVRWNGTLTLALP"
     gene            16398..17219
                     /gene="gspN"
                     /locus_tag="PKN51_07570"
     CDS             16398..17219
                     /gene="gspN"
                     /locus_tag="PKN51_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNT84995.1"
                     /translation="MKRLAPAPRPAGAAAPPPRAPWGWALVGALLGLLLVGLLAAPAR
                     WLAAGVARASGDMVQLTDAQGSLWGGSARLLLTGGAGSQDRSALPGRVHWRLRPGWGA
                     LALQLRADCCTPSGPLGLRLSPRWGGAALALADAHSVWPAALLAGLGTPFNTLQPQGE
                     LNLTTQALSLHWQAGRASLQGGAELTLRQLSSRVATVQPLGSYRVQLQGGDTPTLTLS
                     TLEGPLRLTGSGQWVGRRLRYSGEARADPGLEAALANLLNLLGRRQGDRALMSFG"
     gene            17281..>19061
                     /gene="gspD"
                     /locus_tag="PKN51_07575"
     CDS             17281..>19061
                     /gene="gspD"
                     /locus_tag="PKN51_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNT84996.1"
                     /translation="MPRLSRRPLPSGARRPHRLPALLLALAGALPLAPSQAQQAATPA
                     ASGGKVTLDFANAEIDAVARTMATLTGRNVVVDPRVKGTLTLTSTTPVSPAEALRLFG
                     AQLRTQGFALVENAGMVLVLPEGEAKLQGGSVSAGAVRGGGQIQTQIFRLTHENANNL
                     VPILRPLISPNNTINVGPGTNALVITDYGDNLQRLGRIIAALDVSGATGVEVVRLQHA
                     VASDLAPLVARLLESGGGGSVATPAPVPGQPGMPAAAAALAGSEGGFRTTLVPEPRSN
                     AIVVRAANPARLALARTLIEQLDQPPARRPDGGSGNIHVVYLKNSDAAKLAVTLRAAL
                     AALPGQSGATGAAATGGGASASAATGQGLTAQLSAAGGTLGGNGLASGPTIDARAANQ
                     PSTGGQIQADPATNSLIISAPEPVYRELRAVIDKLDQRRAQVFVESLIAEVRADRAAD
                     FGVQWQSVLRNNGSTIGVIGTNYSTTRATGGSTTNIFDIAAAVLGYRSSGNVSDLAGA
                     VPNTGFNLALGSRTLGLLANFIQSSGAGNVLSTPTLLTLDNEEAKIIVGQNVPFVTGS
                     YTNTGSSSTSVNPFQTFERKDVGLTLRV"
BASE COUNT         2828 a         6990 c         6654 g         2589 t
ORIGIN      
        1 tgtcgtgcat cttggggatg gcgtccagca gcatgcgcgt gtaggggtgg cgcggcgcgc
       61 tgaacagctc ggccttgggt gccagctcga cgatgcggcc caggtacatc acgcccacgg
      121 cgtcgctgac gtggcgcacc accgccaggt tgtggctgat gaacaggtag gtcaggccac
      181 gctggcgctg cagctccttc atgatgttga gcacctgggc ctgcaccgac acgtcgagcg
      241 cgctggtggg ttcgtcgcag accaggaatt cgggctgggt cgccagggcg cgcgcgatcg
      301 agatgcgctg gcgctggccg ccggagaatt ggtgcgggta ctttacgcca tcggccgccg
      361 ccaggccgac cgattgcagc agctcgccca cgcgctccag cgcctgcgcc ctggacgcgg
      421 ccatgccgtg ctcgtgcagc ggctcggcga tgatgtcggc caccttccag cgcgggttga
      481 ggctggcgta cgggtcctgg aagatcatct ggatgctgcg ccgcaggtgg tgcccgtcct
      541 tgccccggaa ggcggcgtgc gcgtcctgcc ccttgaacag aaagcgcccg cgcgtgggct
      601 ggtacagccc caccagcacg cgcgccacgg tgctcttgcc gcagcccgat tcgcccacca
      661 gcgccagcgt ctggccggcc tggatgctga agctggcttc gtccaccgcg tgcagcaact
      721 ggcgcggctt gccttcgagc aggcggttga gcagcggcgg cgagacgtcg aaggtgcgcg
      781 ccagggcctc ggcctgcacc agcggctggg tcgcgcccgc cggcgccgca gcgggctggg
      841 cggggccggc gtcgatcagg gcgggctgca gcgcggccga cggccacggt gcaccgaaac
      901 gtgggctcgt catgccgcca cctcctgccg cgcatgcagc cagcacgcgg cctgggtggc
      961 gccggccggc agcagatcgg gccgctcgac gcggcagcgc tcgaacacgc gcgggcagcg
     1021 cggattgaag gcgcagccgc gcggaatggc gttcaggcgc ggcatggcgc cgtcgatctg
     1081 gttgagcgtt tcgcggtcct gttccatgtc gggaatggag gccatcagcc ccgccgtgta
     1141 ggggtgggcc gggtggttga tgacttcatg caccggcccg atttcggcca cccggccggc
     1201 gtacatgacg gccacgcggt cgcaggtttc ggcgatcacg cccatgtcgt gcgtgatcag
     1261 catcaccgcg gcgccgcgct cgcggcagac gcgcttgagc aggccgatga tctgcgcctg
     1321 gatcgacacg tcgagcgccg tggtgggttc gtcggccacg atcagcttgg gctcggccgc
     1381 cagcgccagg gcgatcacca cgcgctggcg catgccgccg gaaaactggt gcgggtagtg
     1441 gtcgatgcgc tgctcggcgg cgggaatgcc ggtgtccttg agcaggtcga tggcgcgctg
     1501 gcgcgcttcg gcggcgctca ccggcaggtg ggtctggatg gtttcgacca gctgccggcc
     1561 gacactgtag agcgggttga gggaggtcag cgggtcctgg aagatggcgc cgatcttgcg
     1621 cccacggatg tgacgcatct gctcatgcga gagctggtcg atgcgctggc cttccagcac
     1681 gatctgtccc gaagcgacac gccccggcgg ctccagcagg ccgatgatgg aggcgccggt
     1741 gagcgacttg cccgcgcccg attcgcccac cacgcccagg atctcgcccg gcgcgatctc
     1801 gaacgacaca tcgtccagcg cacgcagcgt gccgcgccgg ttgggaaact caacgatcag
     1861 gttgcggact tcaagcagtg acatagatgt gggttcggcc tcggagacac atgacccatg
     1921 acggcgtcgc tacgcgacag cgcatgacag gatccgagac gcctttgtca ttcaatacgg
     1981 aagccgcgcc agcggcaaag ccatgggtca tttcgtcacc gcaaccgcgg attgagcgca
     2041 tcgcgcagcc agtcgcccag caggttgacc gacagcgcga tcagcaccag catgacaccg
     2101 ggaaagatgg tgatccacca ttcgcccgaa aacaggtagt cgttgccgac gcggatgagc
     2161 gtgcccagcg agggcgaggt gggcggcacg ccgacaccca ggaaggacag cgtggcctcg
     2221 gtgatgatgg cggtggccac ctggatggtg gccagcacca tcaccggccc catgacgttg
     2281 ggcagcacat gcttgagcat gatgcgccag ggcgccacgc cggtcacacg cgcggcctgc
     2341 acgtattcct tgccgcgctc gaccatggtg ctgccgcgca ccgtgcgcgc gtactgcacc
     2401 cagccggtca gcgcgatggc gatgatcagc acgccaaacg ccaccgaagt gccggcgtcc
     2461 ggaaacttgg cccgcgccac gccggccacc agcaaggcga tcaggatggc cggaaacgac
     2521 agcatgacgt cgcacacgcg catcagaaag ccgtccaccc agccgccgcg gaaaccggcg
     2581 atcagcccca ggccgacacc caccagcacc gacagcagca ccgaggccag ccccaccacc
     2641 agcgaaatgc gcgcgccgta catcagcgcc gacaggatgt cgcgcccctg atcgtccgtg
     2701 cccagccagt acttgctgct gccaccttcc atccagaacg gcggcagacg ggcgtcggac
     2761 agctccagcg tggtcaggtc cagcggattg tgcggcgcca cccagggcgc gaacacggcg
     2821 cagaacacgc ataccagccc gaccagcgcc gccacgatgg cggtgggtga gctgcggaag
     2881 ctgtaggcga catcgctgtc ccaccagcgg gcaagtgcat tgctcatgaa atgatagctt
     2941 tctgcgctgt ctgggcgggc gctgcaggct tgtttttcat atgatttcga gtggcacgag
     3001 gtggcgcggg cggcgcctca atggccggcc gccttggcca cgcgcaggcg cgggtcgacc
     3061 accacgtaca gcaggtccac caccaggttg atgaccacga agatcagcgc gatcagacac
     3121 agataggccg ccatcaccgg cacgtcggcg aaggtgaccg cctggatgaa caacaggccc
     3181 atgcccggcc actgaaacac cgtctcggtg atgatggcaa aggcgatcag gccgcccagc
     3241 tgcagaccgg tgatggtcat caccggcacc agggtgttct tgagcgcgtg gccgaaatgg
     3301 atggcgcggt cggtcagccc gcgcgcgcgc gcgaacttga tgtagtcggt gcgcagcacc
     3361 tccagcatct cggcgcgcac caggcgcatg atgagcgtga gctggaagat cgccagcgtc
     3421 agcgccggca ggatcatgtg cagccagcca tcgagcgtga acaaacccga gctccaccag
     3481 ccgatgcgca ccgtctcgcc gcgcccgaaa ctgggcagcc agcccagcca gaccgaaaag
     3541 atcaggatca gcaggatgcc gatcaggaag gtcggcagcg acacccccag cagcgacacc
     3601 gtcatgaaca cctggctcag aaagctgccg cgccgcagcg ccgcatacac gcccatcgga
     3661 ataccgatca gcagggccag cgcggcagcc accagggcca gctcgaaggt ggccggaaag
     3721 cgctcgccca gcaggcgcga caccttggcg ccctggcgca gcgacaggcc gaactcaccc
     3781 tgcaccgcat tcagcaggaa gtgccagaac tgcacgaaga acggctggtc caggcccagg
     3841 tcggcgcgca gctggcggat ctgatcgggc gtggcgtcct gacccagcag gatcagcacc
     3901 gggtcgccca cgtactgaaa cagcaggaag gcgatgaagc ccaccgagac catcaccacg
     3961 atggcctgca gcagtcggcg gagtatgaag gcgcccatgg gtcacgtgca gagtcgatgg
     4021 cggcatcgta gcaaaccggg accagcgccg atagggggca tacgccaatg gtcagaccag
     4081 cgcgttccgg catgacctgg aggaccggac tggcgcaggc gggcacgaaa aagccgcccg
     4141 aaggcggctt ttcgattcga atcatggact gccggttcag gcagcgtcac gatcagaagt
     4201 tgtggcgaac gcccagaccg tagttgttct cgccgtccag acgcaggtag gcggcgtaca
     4261 ggaaggtgcg cttggacagg gcgtacttgc cttccagcag accgttggtg tacttcttga
     4321 ccgggtgctt ggtgtcgcgc gtcaggtcca gcgtgacgga agcggcgccg aaggtggcgg
     4381 tgccaccgat gctgaagccg cggcgcactt tgtggttgtc gctgtaggac gcggcgacgg
     4441 cgaagttgcc gaagttgtac ttgccaccga tggcgtagcc gaccttctga cccttggcct
     4501 tgttggccga cagaccagcg gcgatcgggc cgttggcgta caccacgttc aggtcccact
     4561 tggcggcgcc ggcgttgtcg tccttgaaga tgtagcccag ctcggcagac aggccaccca
     4621 tgtcaggcgt cttgtagctg aactggctgc tgttacgcgc gccacccacg tagccgtagg
     4681 tgttgccgac cacggagtag ttggcggtac cggtcagttc ccaggcggcg acgccgtaga
     4741 acgacgggtt cagggtgcga cccatcttga aggtacccca gttgccgccg acccacatgt
     4801 tggcgttacg gctccagaag ccggggcccg aagcggcggt gctgccgtct tccaggctca
     4861 ggccggtttc gaagttgaag ccagccttca ggccgccacc caggtcttcg acgccacgca
     4921 cgcccagacg gctgttgccg ttgttcatgg tgttggcgct gtgcatgttg acgcggcttt
     4981 caccggcgga tttggtcttg cccagaccag cgtcggccac gccgtacagg gtcacggaag
     5041 attgggccat cgcggcgccg gcaaagccca ggaccgccag ggcaatcaga gattttttca
     5101 ttgcaagttc tccaaggtta aacacttagg gcgccggtgc gaagggtccg tgtgcgattc
     5161 ccgccggttc cccgggccaa cctccccgac cgggaagttg acgctattgc accagacccg
     5221 gcagcaactt gcaaagaatt cagcccgcga tcggacagca cagccccgtt ctgttgcaga
     5281 ccgacaacag aacctctggg agcgttgcaa ataaaagaca accctgcccg gccgacggcc
     5341 ttcggggcgg tcttggtccc tggaaacgcc ctgccgggcg ccgggacagc ctccgcctgc
     5401 gcaagcagag gctctggcgt gcgacagccg gcccgggaaa ccccggtcgt cggccgtcgc
     5461 agcctcggcc gcacgcccct tgtcggggcg ccggccatgc ggtcatcaga agttgtggcg
     5521 gacgccgacg ccgtagttgt tgtcgccgtc gtagcgcagg aaggcgccgt agacgaaggt
     5581 gcgcttggac agtgcgtact tggcctcgag cacaccgttg gtgtacttct tgccggtcca
     5641 ctcgttcttg gtgtcgcgcg tcaggtccag ggcgatcgag gtggcaccga aggtggccga
     5701 accgccgatg ctggcgccac ggcggcgcag gccaccggcg tcggacgcct ggttgtacga
     5761 agcggccaga gcgaagttgc cgaagctgta cttgccaccc agctggtagt tggtcttgcc
     5821 accgctgacc ttgttggccg acaggccggc cgcgatcggg ccgttgttgt acatcaggcc
     5881 cagatcccac acgttggcgc cgacgtcgtg cttggagacg taggccgccg cggcggtgaa
     5941 gccaccgaag ttgggcgtga tgtaggcgaa cgcgctgttg gcgcgcgagc cgatgccggc
     6001 gtagcggtag gtcttgccca gggccgagta gttggcggtg ccggtgacct cataggtggc
     6061 ggtggtcagg tagctcggcg tgaactggcg acccagcttg accgtacccc agttgccgcc
     6121 gaccgacagg ttggcctggc gcgcccagaa ggacgaggtg gtggtggcgc cgtcgtccag
     6181 gtccaggccg gtttcgaagt tgaagccgat cttcaggccg gcacccaggt cttccacacc
     6241 gcgcacgccc agacggctgt cgccgttgtt catggtgctg gcgctggtga attgggcctt
     6301 gttggacgag tcgttgccgc cggacttgat cttgccgatg ccggcgtcgg ccacgccgta
     6361 cagcgtgacg gacgactggg ccatggcggc gccggacagg gccacgacgg ccagggcgat
     6421 gagggatttt ttcattgcag tttctccagg taatgcgaga cgcgaaacgg acgctcagga
     6481 agcaacgaat gacaattcat ggctggccct tgaacgccgg tcgtcacccg atcaggaggg
     6541 atgtcgatca ccacccgaac gtgaggcaat tctatgccat gggcggtttg taaactagta
     6601 tttaccctcg ctttgttgct tttcagccac tgctaggggt ttactgacta gcgggtcatt
     6661 atgacatgca ccatctggtg cagtgtttcg cgctgccagg tacttgtagc gagtcgccaa
     6721 ggggccaact gcgcggcgcg tggccgcagg catggtggag ccatgtcaag gccacgccac
     6781 aaagcagatg gccccttggc ggctcgccct gcgggaagaa aaaaaaaggc ttgcggcggc
     6841 gttgcgccgc ttgccaaggc cgccagcatg ggtggccagc cgcgccttgc tgcacgcctt
     6901 gttctggctt ctgtaagtgc ctgacagcgc gaaacactgc actgggtttg ccctaacatc
     6961 gcgccatgcc gactgccatc gatcgcctgc tcgccgccgc cgacagcggc ctgcgcacgc
     7021 tgctggcgcc gccgcgcgcc gcccgtccca gcccggccgc cgccatgacc gcatcgacac
     7081 ccctgaccga ggacgagcag cgcctgtcgg gggcgctgat gcgggtcaac cacgtgggcg
     7141 aaatctgcgc ccaggcgctc tacaccgcgc aggcgctggg caccagcgac ccggcgctgc
     7201 gccggcagct cgacgccgcc gcacgcgagg aaaccgacca cctggcctgg acgcgcgagc
     7261 ggctcgacca gctgggcgac cgcgtttcgc tgctcaaccc gctgtggttt gccggcgcct
     7321 tcggcatcgg cctgctggcc gggcgcatcg gcggcgaccg gctgagcctg ggcttcgtgg
     7381 tggagaccga gcgccaggtc gaggcccacc tggccagcca cctggaccgt ctgccggcgg
     7441 ccgacagcgc ctcgcgcgcc atcgtggcgc agatgaagga cgacgaggcg cgccacgccg
     7501 acgccgccct gcacgccggc ggcgtggcgc tgccggcgcc ggtgcgcggg ctgatgcggc
     7561 tggcggccaa ggtgatgacg gtcaccgcgc accgcatctg agcggtttgc agcaccaaat
     7621 cgtggcgtaa cgccctactg gacagcgcca gcagctttca tttcgatagc aatcggcggg
     7681 cgtcagaggc tgagagtttt tcgttcttgc ccgaaaggcg cgtcagaaca ccgccgatcc
     7741 tggctgcaca agcacagcct tggcccgagc catggcgagc atttgcaacg acgagcagcg
     7801 gtgttttggc gtgccaggcg ggcatggaca aaagacgctc aacctctcag acctccacca
     7861 cctcgaatga agtggtgatg gtcgccgtct tgcccagcat gatcgaggcc gagcagtact
     7921 tgtcgtggct gagctggatg gcgcgctcca ccgccgccgc cggcacgccg cggccgctga
     7981 cggtgaagtg catgtggatg gcggtgaaca ccttggggtc ttcgctggcg cgctcgctgc
     8041 tcaggcgcac cgagcagccg cgcacgtcgt ggcggccgcg ccgcaggatc agcaccacgt
     8101 cgtaggcggt gcagccgccg gtgccggcca gcagcgtctc catcgggcgc ggcgccaggt
     8161 tctggccgcc gaattcgggc cgcgcggcat cgggcgcgcc gtccatggcg atcacgtgac
     8221 cggagccggt ttccgccaca aaccccatgc ccgagcgggt gcccgccgcg cccgtccagc
     8281 tcaccgtgca ttccatgtgc gccatccttt ccgtgtcctg attgcaggcc gcaagcgtag
     8341 cggatgcggc gcgcaccccg cgccagccga gcaaaagtac cgctttcgcc acagtcccgc
     8401 tgttgcattg cagcaaacac ttggcgaacg gtcgtgcttt tcgcctatac ttgcgttcat
     8461 gcgagtgagg tttcattcgc acagcttgtc tcctccaccc cttcaacaag gtggattcaa
     8521 gccccggcgc tgcggctccg gggctttttt ttgctgcacc gcaccgtgcc agggcgccgc
     8581 cggtgccccg gaccgtatga tttcggcttc attcgcgcgc ccacccgctt catgacaccg
     8641 cccgcctccg cgcccaccgg cgccgccgcc ctgagcccga ccgactacct caagcaggtg
     8701 ctgaccgccc gcgtctacga cgtggcggtg gagtccgacc tcgacccggc gcgcagcctc
     8761 agccgccgcc tgcacaacaa ggtgacgctc aagcgcgagg accagcagcc ggtgttcagc
     8821 ttcaagctgc gcggcgccta caacaagatg gcgcacctga cgcccgagca gctgcacaag
     8881 ggcgtgatct gcgccagcgc cggcaaccac gcccagggcg tggcgctggg agcgcacaag
     8941 ctgggcacgc gggcggtgat cgtgatgccc accaccaccc cgcaggtcaa ggtggacgcc
     9001 gtgcgggcgc tgggcggcga ggtggtgctg gccggcgaca gctactccga cgcctacgag
     9061 cacgccctgg gcctgcaggc cgagcagggc ctgaccttca tccacccgtt cgacgacccg
     9121 ctggtgatcg ccggccaggg cacggtgggc ctggagatcc tgcgccagca caaggggccg
     9181 ctgcacgcgg tgttcgtgcc catcggcggc ggcgggctga tcagcggcgt gggcgcctgc
     9241 atcaaggcgg tgcggcctga ggtcaaggtg atcggcgtgc agatgagcga ctccgacgcc
     9301 atggcgcagt cggtgcgcca gggccgccat gtgcagctgg ccgacgtcgg cctgttcgcc
     9361 gacggcaccg ccgtcaagcg tgtgggcgac gaaaccctgc gcctggccgg cgcggtggtg
     9421 gacgagttca tcaccgtcga caccgacgcc gtgtgcgccg ccatcaagga cgtgttcatc
     9481 gacacgcgca gcatcgtcga gccgtccggc gccatggcgg tggcggccct caagcagtac
     9541 gtggccacgc acaagacacg cggcgaaagc tacgtggcgc tgctgtcggg cgccaacatg
     9601 aacttcgacc gcctgcgctt cgtcgccgag cgcgccgagg tgggcgagga gcgcgaggcg
     9661 ctgttcgccg tcaccatccc cgaggcgcgc ggcagcttcc ggcgctttct ggaggtgatc
     9721 ggcagcctgc cgggtggcgc gcgcaacgtg accgagttca actaccgcat cagcgacgcc
     9781 gcgcaggcgc acgtgttcat cggcctgacc accgccgcgc gcggcgaaag ccccaagatc
     9841 gccgcccact tcgagcgcca cggcctgaag acggtggacc tgacgcacga cgagctggcc
     9901 aaggagcatg tgcgccacat ggtcggcggg cgctcgtcgc tggcgcagca cgagcggctg
     9961 ctgcgcttcg tgttccccga gcgccccggg gcgctgctga agttcctgtc gctgatgcag
    10021 ccgagctgga acatcagcct gttccactac cgcaaccagg gcgccgacta cggccgcatc
    10081 ctggtcggca tgcaggtgcc ggccagcgac gacgccgcgt tccggcgctt tctggccgag
    10141 ctgggctacc cctgggtcga ggaaaccgac aacccggcct accggctgtt cctgcagcgt
    10201 tgagtcgcgc ctgacccagg gcggcgcagg cgaatgggaa aagcgcgcgc cgagcttcga
    10261 tgacgagggc gccgccgggg cgaaggcggg gttcggccct gccccgggtg cgctgtcgtt
    10321 gatggctgtc ggcgcctttc agccaagggc cggcgcccgc tccccagact ccgcctactg
    10381 cgccggcgcg gcaccaggcg ccggaaaggc ctgcggctgc ggcgacaccg ccatgccggc
    10441 cggcagcgtc ggcggggtcg gcaccacggg tgccatcgga ccggcccgcg acgttcccgg
    10501 gcgcccggcc ggcaccggcg gcgccggatg cgaaggcacc gccgccgcgc tgccgctgct
    10561 gcgccgctcc agcggcggca tctccagccg cagctcgcgg ccaccggctg ccgccagcag
    10621 cgcgccatgc ggcgccacgc tgcgcaggcg ccagccgcct tccacccctt ccagctcggc
    10681 gcccacgcgc agcggccggg ccggctggcc gtccagcgcg atcagcgccg cgccgcgcgc
    10741 gccgtgcgtc accacgccct tgagcaccac ccgaccgccc agttcgggcg cggcggcggg
    10801 cgctgccggc gccgccgccg tggcgcccag cacccgcgcc acctgggcgc tgtccaccgt
    10861 gcgcgcggcc ggcgcgccgc ccgccaccgg cgccggctgc ggcgccgccg gctgcccggc
    10921 gcgcagccac caccacagcg cactgccggc cagcagcgcc cacagcagca gggtcggcag
    10981 gccgacggcc aggcgggaag cgagccgttg ggtcatggcg tggcggcagc gatcgcggtc
    11041 atcgtcgggg attatcattg aacccaccac ggccgtggag cgctcgcccg ctgcggctgc
    11101 gccgcatgga tgtgcaggca ggccggctcg cgcccctgcc cggcacaccg accacgacag
    11161 cggcgggcgc ccccgcggcc gtgctgtatt gaagctcgcc tgcagacccc cgccatgacc
    11221 ctctcgcccc ccatcgccgc ccgcgcgcgc cgcgccgccg gcttcaccct gatcgagctg
    11281 atggtggtgc tggtcatcat cggcgtgctg gcggcgctga tcgtgcccaa cgtgctggac
    11341 cgcaccgacg acgcgcgcgc caccgccgcc cgcaccgacg tgcacaacct gatgcaggcg
    11401 ctcaagctgt acaagctgga caaccagcgc taccccacgg ccgagcaggg cctggcggcg
    11461 ctggtgcagc gtcccagcgc accgcccgcg cccggcaact ggaagcccta cctggagaag
    11521 ctgcccaacg acccctgggg ccagccctac cagtacctga accccggcat ccgcggcgag
    11581 gtcgacgtga tgagcttcgg cgccgacggc aagagcggcg gcgaaggcaa ggatgccgac
    11641 atcggcagct ggcagtgaag gcgctcgccc gagcggctgg ccggtgcctg cgcgccgccg
    11701 ccacggcgcg gcgccggcgg ggctgagcat gcccccggtc gctcctgaaa caggagctgc
    11761 cggcgctgca tggacgggcg ccacagtcta ctttggcatg aaatcccggc gcggcttcac
    11821 cctcatcgag ctgatggtgg tgatcgccat cgtcgccatc ggcagtgcgc tggccagcct
    11881 ggccctgccc gacagcgacc gccgcacgct ggcgcgcgaa ggcgaacggc tggcggcgct
    11941 gctcgaagcg gcccgcgcgc agtcgcgcgc cgccggcgtg ccggtgcgct ggcgcgccac
    12001 cgccagcggc tttgcctggg acggcctgcc gcccggcgcc gcgccgctgc ccaccggctg
    12061 gctggacgcg cgcacccgcg ccctgggcaa cgccgccctg ctgctgggcc ccgacccgat
    12121 catcgacgcg caggcggtgg tgctgacgcg gcccgaccag cacagcccgc aggcgctgcg
    12181 catcgccagc gacggcctgc ggcccttcgg cgtcgcgccg ctgacgccat gagccgccgc
    12241 cgcgccgccg cgccgcccga cgccggcttc acgctggtgg aagtgctggt ggcgctggcc
    12301 atcaccgcca tcgcgctgat cgccggcttc aaggccagcg ccgcgctgac cgacaacgcc
    12361 ggccgccagg gcacggtgct gctggcccag atctgcgccg aaaaccgcct gaccgagctg
    12421 cgcctggcgc gccagctgcc cgccgtcggc gacagcgaac tggcctgcgc ccaggccgag
    12481 cgccagttcc tggtcacgca ggccgtgcgg cccacgccca accccaactt ccgccgcgtc
    12541 gaagccgccg tcagcgaagc cggcgtgccg gtgctgcgcc tgagcaccat cgtgggtcgc
    12601 cactgatgcc ggccgtgggc aacccaccct cgaacgctat tgttacgata gctgacggcg
    12661 caagcctggc aagcgctggc tgccggtttg gctcatcatt gcgcggcccg cgcggcttca
    12721 ccctgatcga gctgctggtg gcgctcagcc tggtcgccgt gatcgccgtg ctgagctggc
    12781 gcggcatcga cgccatggcg cgcgcgcagc agtccaccca gcagcacacc gacgaagtgc
    12841 tggccctgca ggccggactg gcgcagtggc gcgccgacct cgacgccctg atgagctggc
    12901 ccgccgcccc cgccgccccc ggccagccgc ccgccagcgg cccggccagc cagcgcagcc
    12961 tggcctggga cggccgcacc ctgcgcctga cccgcagcga cgccggcgac gcggccgccg
    13021 gcctgcgcgt ggtggcctgg acgcgccgcc ccgccagcgg ccagtggcag cgctggcaat
    13081 cgccgccgct ggcgtcgcag gccgcctggg ccgccgcctg ggacgccgcc gcgcactggg
    13141 gccagtccga caccgaaccg cccgccgccg ccggcgccag cgccgtcacc ctcgcgcgcg
    13201 cgctcgactg gcagttgcac tactaccgcc acaacgcctg gacgcaccca ctgtccagca
    13261 gcgccgaatc gggcagcgcc acccagctgc tgcccgacgg cgtgcgcctg ttcctcaccc
    13321 tggccgcagg ccaggcgctg gccggcccgc tggtgctcga ctgggtgcgg ccggatttca
    13381 gcggagcgca gccatgacgc gccaccaggc gccacgcgca gcgggcaagc gtgggggcca
    13441 cccgcccgcc gccgggccgc tcccaaggcg ggcggcggcc cctgaggggc tgagggctgc
    13501 ggctccagac tcgcctgcgc gagtctggac agcccgaaga gcagctggct ctgacggctg
    13561 cgcggcctgc aaggccagcg caccacgcgc agcgggcaag cgtgggggcc aggaacgcgg
    13621 cgccgccctg ctgatggcca tgctcagcgt ggccctggtg gccaccctgg cggccggggc
    13681 cctgtggcgc cagtggcgcg gggtcgaggt ggaggcggcc gagcgcgcgc gcgcccagtc
    13741 ggcctggatc ctgaacggcg cgctcgactg gagccggctg atcctggccg gcgacctgcg
    13801 cgaatcgggg ctggtcgacc acctgggcga gccctgggcc gtgccgctgg ccgaagcccg
    13861 gctgtcgacc tttctggccg ccggcgaagc cagcaccgac accgaccgcg aagcctttct
    13921 gtccggccag atcatcgacc tgcagtcgcg cctgaacgtg atgaacctgg tgaccggcga
    13981 cggccacgcg cagagccagg cgctgctgcg ctttgcgcgc ctgttcgagc tgctgggcct
    14041 gcccgccgcc gagctgggtg cgctgcagcg caacctggcg cgcgcgcagg ccgccatgac
    14101 cggcagcgcc ggcggcgacg cgccgctgct gccgcgccgc ttcgagcaac tggtctggct
    14161 gggcctgccg ccggccacgc tggcggcgct gcgcccctac gtcaccctgc tgccgaccca
    14221 gggcagcgaa gccacgcgcg tcaacctcaa caccgccagc gcgcaggtga tctgcgccgc
    14281 cgtgcccgaa ctcgacctgg ccgccgccca gcgcctggtg gcggcgcgcg agcgcggcca
    14341 cttcaaggac gtcggcagcg cgctggcgct ggtgccgctg cccggaggcg gcagcgccga
    14401 caccagcggc gtggcggtgg gcacgcagtt cttcgaagtg cgcggccggc tgcggctgga
    14461 ccagctggcg ctggaagaag tctccaccgt gcagcgcgac cccagcacgc gccaggtgcg
    14521 caccctgtgg cgcgagcgcc gcgcgctcgg cacacccgcc gccggtggct gaacgcggca
    14581 cctacaatgc cgcgaccccc atgagcctgc tgctgatcgc cctgccgccg ggcccgccgg
    14641 ccgactacgc cttcgccacc tcggccgacg gccagggcgt gagcgcgcac ggcagcgccg
    14701 cgccggccct gctgccggcc gccgggcgcg gcgtcgaagt ggtggcggtg gtgccggcgg
    14761 cgcggctgtc gtggcaggcg gtgcagctgc cgcgcggcgt cggccccggc gccccgcgcc
    14821 tgcgcagcat cctggaaggc ctgctggaag agcagctgct cgacgagccg gcccgcctgc
    14881 acctggcgct cgcccccggc gcccagggcg gcgcccgcgc ctgggtggcg gtgtgcgagc
    14941 gcgcctggct ggccgcccac ctggcggcgc tcgatgccgc cggccgcccg cccgcccgca
    15001 tcgtgcccga actcagcccc cagcacggcc ccacccgcct gtggctgagc ggcgagcccg
    15061 aacgcccctg gctgctgatg agcggcgacg gcgtgcccgg cggcgcccag gcgctgccct
    15121 tcggccccgg cagcgccgcc ctgttgcccg ccgcggcggc cgacgccccg gcgcccgagc
    15181 tgctggccga gccggcgctg gcggcgctgg ccgaacaggc gttccagcgc ccggtggcca
    15241 tccgctcggc ggccgagcgc ctgctgcacg ccagccgcag cccctgggac ctggcgcagc
    15301 tcgaattcag ccgcggcggc cgcgcccgcg cggcgcggcg cgccggcacc ctgtggcgcg
    15361 acttcctgca cgcccccgcc tggcggccgg cgcgctgggc gctggcgctg ctgctgctgg
    15421 tcaacctggc cggcctcaac ctgctggcgt ggcgcaccgg ccaggcgctg gcggcgcagc
    15481 gccaggccca gcaggtgctg ctgaccgaca ccttcccgca ggtcaaggtg gtggtcgacg
    15541 cgcccgtgca gatggcgcgc gaagtggccg ccctgcgcca ggccaccggc gccgcctcgg
    15601 gccgcgacct ggaaccgatg ttgcaggctt ttgggcagct ggcgcttacc cagcaagcgc
    15661 cgacagctat cgaatttgaa gcgggcgagc tgcgcctgaa aggcctcacg ctggccgccg
    15721 gcgcgctggc cgaagccaac ccgaggctgc gcccgctggg cctgcacctg cggaccgaag
    15781 gcgacaccgc ggtgctgcgg caggagggcg caccatgagc gcgacgggcc gttcccaagc
    15841 gcgaatcccg ccgcgcaacg cacggagggg cagcgcatga gcgcgccagc cagcgccgcg
    15901 gcgcccacgc gcggcccgcg cgccctgggc gcggcctggg cgcgcctggc cgcgcgcgag
    15961 cgccgcctgg tgctgctggc cgccgccgtg ctgggcctgg ccctgttgtg gctgctggcc
    16021 gtcgcgccgg cgctgcgcac gctgcgccag gccgaggcgc agcgcgccac gctggcggcg
    16081 cagaccgagc agctgcagcg gctgcgcgcc gaagcgcagg cgctgcaggc gctgccccgc
    16141 ctgggccatg acgaggcgct gcgcgccctc gaaagctcgg tgcgccagcg cctgggcgac
    16201 agcgcccagc tcagcgtggt gggcgagcgc gccaccctgg tgctgcgcca gaccagcgcc
    16261 gaggcgctgg cgcagtggct ggccgacgcc cgcgccaacg cccgcgccaa cacggtcgaa
    16321 gcgcggctca cgcgcgccgg cgacaacgct cccggcgcgc cggtgcgctg gaacggcacg
    16381 ctgacgctgg cgctgccatg aagcgcctcg cgcccgcgcc gcgccctgcc ggcgccgccg
    16441 cgccgccgcc ccgcgcgccc tggggctggg cgctggtcgg ggcgctgctg ggcctgctgc
    16501 tggtcgggct gctggcggcg ccggcgcgct ggctggccgc cggcgtcgcc cgcgccagcg
    16561 gcgacatggt gcaactgacc gacgcccagg gcagcctgtg gggcggctcg gcgcgcctgc
    16621 tgctgaccgg cggcgccggc agccaggacc gcagcgccct gcccggccgc gtgcactggc
    16681 gcctgcggcc gggttggggg gcgctggcgc tgcaactgcg cgccgactgc tgcaccccgt
    16741 ccggcccgct gggcctgcgc ctgagcccgc gctggggcgg cgccgcgctg gcgctggccg
    16801 acgcccacag cgtctggccg gcggccctgc tggccggcct gggcacaccg ttcaacaccc
    16861 tgcagccgca gggcgagctg aacctgacca cgcaggcgct gtcgctgcac tggcaggccg
    16921 gccgcgccag cctgcagggc ggcgccgaac tgacgctgcg ccagctgtcc tcgcgcgtgg
    16981 ccaccgtgca acccctgggt tcgtaccgcg tgcagctgca gggcggcgat acgccgacgc
    17041 tgaccctgtc cacgctggaa ggcccgctgc gcctgaccgg ctccggccaa tgggtgggcc
    17101 gccgcctgcg ctacagcggc gaagcgcgcg ccgaccccgg cctggaagcg gcgctggcca
    17161 acctgctcaa cctgctcggc cgccgccagg gcgatcgggc cctgatgtcc ttcggctgag
    17221 cggcgcctgt ccgcgccgcc atttgctaca gtgcaacacg ctttgcctcc gaccccgccc
    17281 atgccacgcc tgtcccgccg acccttgccg tccggcgcgc gccgcccgca ccgcctgccc
    17341 gccctgctgc tggcgctggc gggcgcgctg ccgctggccc ccagccaggc acagcaagcc
    17401 gccacgccgg ccgccagcgg cggcaaggtg acgctggact tcgccaacgc cgagatcgac
    17461 gccgtggcgc gcaccatggc cacgctgacc ggccgcaacg tggtggtcga cccgcgcgtg
    17521 aagggcaccc tgacgctgac cagcaccacg ccggtgtcgc cggccgaggc gctgcgcctg
    17581 ttcggcgccc agctgcgcac ccagggcttt gcgctggtcg aaaacgccgg catggtgctg
    17641 gtgctgcccg agggcgaagc caagctgcag ggcggcagcg tgtcggccgg cgcggtgcgc
    17701 ggcggcggcc agatccagac ccagatcttt cgcctgacgc acgagaacgc caacaacctg
    17761 gtgcccatcc tgcgcccgct gatcagcccc aacaacacca tcaacgtcgg gcccggcacc
    17821 aacgcgctgg tgatcaccga ctacggcgac aacctgcagc ggctgggccg catcatcgcc
    17881 gcgctggacg tgtcgggcgc caccggggtc gaagtggtgc gcctgcagca cgccgtggcc
    17941 tccgacctgg cgccgctggt ggcgcgcctg ctcgaatcgg gcggcggcgg cagcgtcgcc
    18001 accccggcgc cggtgccggg ccagcccggc atgcccgcgg cggccgccgc gctggccggc
    18061 agcgaaggcg gctttcgcac caccctggtg ccggagccgc gcagcaacgc catcgtcgtg
    18121 cgcgccgcca acccggcgcg gctggcgctg gcgcgcacgc tgatcgagca gctcgaccag
    18181 ccgccggcgc gccggcccga tggcggcagc ggcaacatcc acgtcgtgta cctgaagaat
    18241 tccgacgccg ccaagctggc cgtcacgctg cgcgccgcgc tggcggccct gccgggccag
    18301 agcggcgcga cgggcgcggc cgccaccggt ggcggcgcca gcgccagcgc cgccaccggc
    18361 caggggctga cggcccagct cagcgccgcc ggcggcacgc tcggcggcaa cggcctggcc
    18421 agcggcccga cgatcgacgc ccgcgccgcc aaccagccgt ccaccggcgg ccagatccag
    18481 gccgacccgg ccaccaattc gctcatcatc agcgcgcccg agccggtgta ccgcgagctg
    18541 cgcgccgtca tcgacaagct cgaccagcgc cgcgcccagg tcttcgtcga aagcctgatc
    18601 gccgaggtgc gcgccgaccg ggcggccgac ttcggcgtgc agtggcagag cgtgctgcgc
    18661 aacaacggca gcaccatcgg cgtcatcggc accaactact cgaccacccg cgccaccggc
    18721 ggcagcacca ccaacatctt cgacatcgcg gcggcggtgc tgggctaccg cagcagcggc
    18781 aacgtctccg acctggcggg agccgtcccc aacaccggct tcaacctggc gctgggttcg
    18841 cgcaccctgg gcctgctggc caacttcatc cagagcagcg gcgccggcaa cgtgctgtcc
    18901 acgcccaccc tgctgacgct ggacaacgaa gaagccaaga tcatcgtcgg ccagaacgtg
    18961 cccttcgtca ccggcagcta caccaacacc ggcagcagca gcacctccgt caacccgttc
    19021 cagaccttcg agcgcaagga cgtcggcctg acgctgcgcg t
//
LOCUS       DAOQTZ010000050        18929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3369_length_18929_cov_12.982781, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000050 DAOQTZ010000000
VERSION     DAOQTZ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3369_length_18929_cov_12.982781"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1167
                     /locus_tag="PKN51_07580"
     CDS             49..1167
                     /locus_tag="PKN51_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNT84997.1"
                     /translation="MPQPPDLDLLLRAVLLGTFALSAVFGAIMHRTGFCTMGAISDVV
                     NMGETTRLRQWALAAGVATLGFGVLAAGGWVQPADTVYAGPRWLWLSGLVGGLLFGVG
                     MVLASGCGARMLVRLGGGNLKGLIVFLVMGLASYATLRGITAVLRDRTVDQVEWVFNV
                     PATLGHLAASAGGWPLPVGLLGAALLVGGLLMAWALTGRGFINGNNLLAGLGLGGVIV
                     ALWWLSGHLGYLPEHPDTLEPAYLGTRSGRMEALTFTGPLAHTIDWFIFFSDRSQRLS
                     LGVVAVFGLVAGAALHALASRRFRWEGFRTTEDLANHLIGGTLMGVGGVTALGCTIGQ
                     GLSGISNLSLTSFTALAGIVGGAVLALKYQGWRLERSL"
     gene            1164..1952
                     /locus_tag="PKN51_07585"
     CDS             1164..1952
                     /locus_tag="PKN51_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNT84998.1"
                     /translation="MSAAAVEADLERRFGGLARLYGAEGAARIRAAHVAVVGIGGVGS
                     WAAEGLARSGVGQITLIDLDHVAESNVNRQIHALTPTFGQAKVQAMRERIGQIHPACV
                     VHAVEDFVAPTNWPQLLPAPVHAVIDACDQVSVKLAMAAWARERGLCFVTVGAAGGKR
                     HGERVEVADLAEVTHDPLLAQLRYRLRRQHGAPTGGKRLGVTAVFSRESVRLPEACEA
                     PQQVDSSLNCHGYGSSVAVTATFGLCAAGWVLDRLSQEGQKIRL"
     gene            2000..2075
                     /locus_tag="PKN51_07590"
     tRNA            2000..2075
                     /locus_tag="PKN51_07590"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2033..2035,aa:Lys,seq:ttt)
     gene            complement(2164..3117)
                     /gene="gluQRS"
                     /locus_tag="PKN51_07595"
     CDS             complement(2164..3117)
                     /gene="gluQRS"
                     /locus_tag="PKN51_07595"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNT84999.1"
                     /translation="MYVGRFAPSPTGPLHAGSLVAALASWLDARAHDGRWLVRIEDVD
                     TPRCVPGADRQMLQQLAACGLPPDEPPVWQSGRSAHYQAALDALVTAGAAYPCGCSRK
                     DIEQAQAARGLRRQRGVELPYPGSCRPERGGLQGKPARAWRLHTGVHESNPPPGQSGQ
                     APAAIVSEATNEAIVWHDRRLGPQRQDVAHEVGDFVLRRADGLWAYQLAVVVDDAAQG
                     VTHVVRGADLADNTPRQILLQRRLGLATPRYLHTPLVLAADGEKLSKQHGAQALDVSD
                     PLRTLQRAAAVLALDPKAGTSVPEALAAWTAQWRQRWVQAD"
     gene            complement(3158..4225)
                     /locus_tag="PKN51_07600"
     CDS             complement(3158..4225)
                     /locus_tag="PKN51_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT85000.1"
                     /translation="MTPPRPHLSRRIAVVGAGIAGLACARTLRQAGHEVAVFEAAPQP
                     GGRMATVQTAFGGFDAGVQYFTVRDERFMQALQATASDVVRRWSANAVRVLDERGRVV
                     EAAPPAPESHWVAVPAMDRLPQRWAEPLLAAGALHLDTRVWRVAHDRLSPGRWQLHTD
                     GPGEDHPVQAGFDQVLLAIPPQQANELLQASTLSTAPFAALGAVDVAPCWTLMLAYPN
                     ATQPGLHTLGPQWNAARSTHHRVSWLARESSKPGRVAVERWTVQASAAWSREHERDDP
                     ARVSAKLLRAFAEITGIHATPAHTEALLWRQARTLASLGRTHLWDGAGSIGLCGDWCL
                     GSRVEDAFVSGLELALAVIAA"
     gene            4373..7870
                     /gene="dnaE"
                     /locus_tag="PKN51_07605"
     CDS             4373..7870
                     /gene="dnaE"
                     /locus_tag="PKN51_07605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNT85001.1"
                     /translation="MFVHLRLHTEFSVVDGTTRLDDIVQAAADDGQPALAITDLGNLF
                     GAIKFYKAARGAGVKPIIGAEVLLEGQGSDPEAPTRVLLLVTSGQGYLNLSELLARAW
                     TQGVVKGQAVVRWAWLGELADGLILLSGAQAGPVGQALAQGGVEQASALALQLAELFP
                     HRFYLELQRAGRADDEAHVAAAVQLAARLKLPVVATHPVQFARADDYEAHEARVCIAE
                     GEILGNPRRVRRFTPEQYFKSTAEMQQLFADLPAALSNAAEIARRCNLTLALGKPRLP
                     DFPVPQGHTVETYFRQTSTDGLEQRLAHLYPDPSQRDAERPRYLQRLEFEIDTILKMG
                     FPGYFLIVGDFINWAKNHGCPVGPGRGSGAGSLVAYALKITDLDPLQYKLLFERFLNP
                     ERVSMPDFDIDFCQANRDRVIDYVKDKYGRDAVSQIATFGTMAARAAIRDVGRVLDMS
                     YTFCDGISKLIPNKPGQHITIDGAMKVEPLLAERYRKEDEVRTLIELAQKLEGMTRNV
                     GMHAGGVLIAPGKLTDFCPLYQQPGSEAAVSQYDKDDVEAVGLVKFDFLGLATLTILE
                     IAKELIVERHPGQQHFAYENLPLDDAPTYQLFAQGRTEAVFQFESRGMQGMLRDARPT
                     RLEDLIALNALYRPGPMDLIPSFVARKHGREEVVYPHPLVADMLSETYGIMVYQEQVM
                     QTAQILGGYSLGGADLLRRAMGKKKPEEMAQHRQIFREGAARNGISEGKADEIFDLME
                     KFAGYGFNKSHAAAYSLLAYHTAWLKVHYTAEFYCANMTVEMDNTDKLKVLYEDAQRE
                     GMRFESPDINRGGYRFEPVSNTLIRYGLGAIKGTGQQAIEAIVAAREGRGEGPAGAER
                     GPFTSLFDFCRRVDRTRVNKRTVEALIRAGAFDALQLNRAALAASIDLAFDFAAASEA
                     NVHQGGLFDLMEDGHGSSSQEPELPAVTPWGVKERLSLEKTAIGFYLSGHLFDEVERE
                     VRQFARRSIAELADSREPQLLAGIVGDLRIINGQRGKLALFRLDDKSGVIEARAEEAL
                     IHRHRHILKDDEFIVVMGKAMPDRFSGGLQLNVTQIWDLAQARCRFGKYLRVAVAGKA
                     PEVRRLLAEYPPQVEQTEHGELLRGLPVRLLLERRGSEGGVMAQLHLGEQAQFFPSDA
                     ALASWTAQADEGRAMVVYE"
     gene            complement(8198..8761)
                     /locus_tag="PKN51_07610"
     CDS             complement(8198..8761)
                     /locus_tag="PKN51_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT85002.1"
                     /translation="MSEQREEERGVVWAVLIGVVLLAIGLAVGMGLYRTGKSVAPAAA
                     SSAANANANAGGTAAGGAAGAPANTAAMTSGEVGDGAAIRVENGVVKFYFASGSAELA
                     AGAAEALGEVVKGVAAGKKAVISGFHDVTGDPVRNEELAKQRALAVRDALAALGIGED
                     KIDLRKPAVTTASGSNAEARRVEVHLE"
     gene            8806..9456
                     /locus_tag="PKN51_07615"
     CDS             8806..9456
                     /locus_tag="PKN51_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNT85003.1"
                     /translation="MRSADAPLPPAYADFAHLRAPGRDPLRLLEHTRAQWHRRAGPQA
                     DLWVFGYASLIWRPDFDFSERHATRVHGWHRALAMWSRVNRGTPEQPGLVFALLPGGS
                     CQGMVFRVPRGTADAVFDTLWAREMPSGVYDARWLPCATPHGIVWALAFTLSRRSPSF
                     TGALTPEQYRHIFRHARGRYGTTLDYARRTLETLGAHGIHDRRLRTLLSLVDDEEA"
     gene            9622..10257
                     /gene="pdxH"
                     /locus_tag="PKN51_07620"
     CDS             9622..10257
                     /gene="pdxH"
                     /locus_tag="PKN51_07620"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNT85004.1"
                     /translation="MKDIAHLRKSYERAELSEDASHADPGRQFAQWFEEARQSELPEP
                     NAMTLATVGSDLRPSTRVVLIKEFDERGIVWYTNYESRKGRQLAGNPYAALQFHWVEL
                     ERVVRIEGLVEQVSEAESDAYYASRPLDHRLGAWASPQSQVIPGRAWLAAEAAKVGAR
                     FLLNPPRPPHWGGFRLRPDRWEFWQGRKSRLHDRLCYRLEAGGWVRERLAP"
     gene            10650..11915
                     /locus_tag="PKN51_07625"
     CDS             10650..11915
                     /locus_tag="PKN51_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNT85005.1"
                     /translation="MEPVRLMREMFEAAIASAQPARCVPAHLPNPAEVRGRLIVVGAG
                     KASAAMARAVEEHWRGPLDGLVVTRYGYHVPCQHIRIVEAAHPVPDAAGLQAAERMLG
                     LVSGLTPDDVVLCLISGGGSSLLPLPLPGITLADKQQVNRALLASGASIGEMNTVRRH
                     LSAIKGGRLAAACHPARVITLVLSDVPGDAPMDIASGPTVGDPTTCADALDIVQRYGI
                     ELPAPVRVLLDSGRGESVKPDDARLAGHTTRMVAAPQQALEVAAQVARRHGYAAHILS
                     DAVEGEAREVGKVLAALALQVAGRGQPFTAPCAILSGGETTVTLRGQGRGGRNVECLL
                     SLAIATRGHARIHALMGDTDGVDGAQEVAGAHIGPDTLARAFAQGIHPRRSLDDNDAH
                     GFFQALGDAVVTGPTLTNVNDFRVILVNA"
     gene            12189..13325
                     /gene="ribBA"
                     /locus_tag="PKN51_07630"
     CDS             12189..13325
                     /gene="ribBA"
                     /locus_tag="PKN51_07630"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNT85006.1"
                     /translation="MNAPLSSPAAASAISPVPDIVAELAAGRMVILVDEEDRENEGDL
                     ILAADHVTPEAINFMARFGRGLICLTLTRERCERLRLPPMVTRNGTKMGTAFTVSIEA
                     AEGVTTGISAADRARTVQAAVAAQARADDLVQPGHIFPLQAVDGGVLMRAGHTEAGCD
                     LAAMAGCTPAAVICEIMKDDGTMARLPDLQVFAAEHGLKIGTIADLIEYRSRTESLIE
                     KIASRPLRTAHGEFIAHAYRDQPSGALHLGLVLGQWQPGDTVPVRVHEPLSVLDALEV
                     QRGSHSWSVHESLQHIQSAGRGVAVFLNCGEDAGQLLAQFQGTARAAHAPERGRMDLR
                     SYGVGAQILRDLGVQRMQLLGTPRRLPSMAGGYGLEVTGYITKD"
     gene            13337..13801
                     /gene="ribH"
                     /locus_tag="PKN51_07635"
     CDS             13337..13801
                     /gene="ribH"
                     /locus_tag="PKN51_07635"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNT85007.1"
                     /translation="MLGADKGQTLSLDGSALRIGIVQARFNEGVTQALADACLGELAA
                     LGVPAAQIVHHTVPGALEVPLALQWLARRGECHALIALGCIIRGETYHFELVANESGA
                     GVTRVALDLDIPVANAILTTENLAQAQARQTDKGRDAARVAVEMALLKAGLR"
     gene            13798..14340
                     /gene="nusB"
                     /locus_tag="PKN51_07640"
     CDS             13798..14340
                     /gene="nusB"
                     /locus_tag="PKN51_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNT85008.1"
                     /translation="MSPAPTTPRAAGAAPRPGEGGTRKSSVKSPRSRAREFALQGLYQ
                     HLVGGGDAAAIDVFTRELQGFAKADAAHFDALLHGCIELADDLDALIVPLLDRRLADI
                     SPIERGCMWIGAYEFLKCPDVPWRVVLNECIELAKDFGGTDGHKYVNAVLNGLAPQLR
                     APEVAADRARVRAAKGRDAT"
     gene            14337..15554
                     /locus_tag="PKN51_07645"
     CDS             14337..15554
                     /locus_tag="PKN51_07645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT85009.1"
                     /translation="MRISARAQRIEPFYVMEMAKSAAAIAREAAHGDRPMIYLNIGEP
                     DFTAPPLVQAAAERAIRDGHTQYTQATGLPLLRERISQWYADRFGLRVPARRIVVTAG
                     ASAALQLACLALIEAGDEILMPDPSYPCNRQFVSMAEGRAVLLPSTAAERFQLSADKV
                     RQAWGAHTRGVMLASPSNPTGTSIHPDELARIHAVVSERGGITLIDEIYLGLSYDDTY
                     GHSALALDDQIISINSFSKYFNMTGWRLGWLVVPDALVEVVEQIAQHLFICASTVSQH
                     AALACFEPDSLAEYERRRAEFRARRDWFVPQLGALGLTVPVMPDGAFYAWADCSAACD
                     RMFAGRSEYNSEMQHPGSWEFAFELMKRAHLAVTPGRDFGHAETARCVRFSTANSMAQ
                     LHTAIERLKAVLA"
     gene            15566..15982
                     /gene="ybgC"
                     /locus_tag="PKN51_07650"
     CDS             15566..15982
                     /gene="ybgC"
                     /locus_tag="PKN51_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HNT85010.1"
                     /translation="MAEFEFPIRVYWEDTDAGGIVFYANYLKFFERARTEWLRAGGVH
                     QRSLREQVGGMFVVVESQVKYLRPARLDDELVVTARLQEAGRAGLVIDQQAFVRRQAP
                     EPRELLCRARIRVGWVDAVTLKPARIPPDILKTLPT"
     gene            15979..16692
                     /gene="tolQ"
                     /locus_tag="PKN51_07655"
     CDS             15979..16692
                     /gene="tolQ"
                     /locus_tag="PKN51_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNT85011.1"
                     /translation="MNQDLSIIQLVLHASFVVQLVMLLLLVVSITSWAAIIRKLRSFK
                     QTRALNDEFERDFWSGTSLNDLYASAVQNAKHAGPMERIFASGMREYQKLRERRIGDP
                     SALLDGARRAMRASQQRELDEAESNLSFLASVASVSPYVGLFGTVWGIMHAFTGLASL
                     QQVTLATVAPGIAEALVATAIGLFAAIPAVVAYNRFARELDRLAIKLETFIEEFSNIL
                     QRNLGVHAQAAHSTTPTGY"
     gene            16697..17107
                     /locus_tag="PKN51_07660"
     CDS             16697..17107
                     /locus_tag="PKN51_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HNT85012.1"
                     /translation="MPAVSSRGRGRRAMNEINMVPFIDVMLVLLIIFMVTAPLITPGV
                     INVPSAGQSSKAPDQRVDVQIDADGKLSLAGQIQRSGLGDKEAVAAIRALLADNPELP
                     VMIAADSELRYQQVMNMMKLLREAGVQRVGLAVK"
     gene            17118..18179
                     /gene="tolA"
                     /locus_tag="PKN51_07665"
     CDS             17118..18179
                     /gene="tolA"
                     /locus_tag="PKN51_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNT85013.1"
                     /translation="MNVAAIERAEFAPPPQGGFGRAFGLALAAHLLLILALTWGLRWK
                     NEPQDLAVQAELWSPTVQRAAPKAAPRAPAPQVEPPPPPAALPQPRPEPALKPPPPQV
                     DEQAQRDADIALARKKQEEARQAAAQREQARREREKKEQAEQERRELAQKKAAEEQKR
                     KQDELRKLAAKKQAEEAEQRRQAAAEAKKKAEAQKRAEAEAERKAEAQAKAKAAAQAR
                     AAEDARHKANVARSLAMAGSADSEGGSAAGKGRGGGSAGGQDERDAGPSGNYAGRIRA
                     AVRPNIVFTDDAPGNPVAEVEVRTDQAGNIISRRITRSSGVRTWDDAVLRALDRTEKL
                     PSDNGRYWNPMLITFRLRD"
     gene            complement(18283..18696)
                     /gene="gloA"
                     /locus_tag="PKN51_07670"
     CDS             complement(18283..18696)
                     /gene="gloA"
                     /locus_tag="PKN51_07670"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNT85014.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTQVLGMQLLRTSENPEYRYSLAFV
                     GYEGGNPGQAEIELTYNWGTDSYDLGTAYGHIAIGVPDAYAACERIRAAGGQVTREAG
                     PVKGGTTVIAFVTDPDGYKIELIQRAEGAAGGGLR"
BASE COUNT         2873 a         6521 c         6655 g         2880 t
ORIGIN      
        1 ggcgggcgcg tccaactgcc gcttctaggt tcaatagaat cgcgccccat gccgcaaccg
       61 cccgacctcg acctgctgct gcgcgccgtg ctgctcggca ccttcgcgct gtcggcggtg
      121 tttggcgcca tcatgcaccg caccggcttt tgcaccatgg gtgccatcag cgacgtcgtg
      181 aacatgggcg agaccacccg gctgcgccag tgggcgctgg ccgccggggt ggcgacgctg
      241 ggtttcggcg tgctggccgc cggtggctgg gtgcagccgg ccgacacggt gtacgccggc
      301 ccgcgctggc tgtggctgtc gggcctggtg ggtgggctgc tgttcggcgt cggcatggtg
      361 ctggcctcgg gttgcggcgc caggatgctg gtgcgcctgg gcggcggcaa cctgaagggc
      421 ctgatcgtgt ttctggtcat ggggctggcg tcctacgcca ccttgcgcgg catcacggcg
      481 gtgctgcgcg accgcacggt ggaccaggtg gaatgggtgt tcaacgtccc ggccacactg
      541 ggacacctgg ccgccagcgc gggcggctgg ccgctgccgg tgggcctgct gggggcggcg
      601 ctgctggtgg gcggcttgct gatggcctgg gcgctcaccg ggcgcggctt catcaacggc
      661 aacaacctgc tggccggcct gggcctgggc ggtgtcatcg tcgccctgtg gtggctgagt
      721 ggccacctgg gctacctgcc cgaacacccg gatacgctgg agccggctta cctgggcacg
      781 cgcagcggcc gcatggaggc gctgaccttc accggcccgc tggcgcacac catcgactgg
      841 ttcatcttct tcagcgaccg cagccagcgc ctgagcctgg gcgtggtggc ggtgttcggc
      901 ctggtggccg gggcggcgct gcatgcgctg gcgtcgcggc gctttcgctg ggaaggtttt
      961 cgcaccaccg aagatctggc caaccacctg atcggcggca cgctgatggg tgtcggcggg
     1021 gtaacggcgc tgggctgcac catcggccag gggctgagcg gcatctccaa cctcagcctg
     1081 accagcttca ccgcgctggc gggcattgtc ggcggcgccg tgctggcgct gaaataccag
     1141 ggctggcgcc tggagcgatc gctgtgagcg cggcggccgt cgaggccgat ctggaacgcc
     1201 gcttcggcgg tctggcgcgg ctgtatggcg ccgagggcgc ggcgcgcatc cgcgccgccc
     1261 acgtggcggt ggtcggcatc ggcggcgtcg gctcctgggc cgccgaaggc ctggcgcgca
     1321 gcggtgtcgg gcagatcacg ctcatcgacc tcgaccatgt ggccgagtcc aacgtcaacc
     1381 gtcagatcca tgcgctgaca ccgacgttcg gccaggccaa ggtgcaggcc atgcgcgagc
     1441 gcatagggca gatccatccg gcctgcgtgg tgcatgcggt ggaggacttc gtggccccga
     1501 ccaactggcc gcagctgctg ccggcgccgg tgcatgccgt catcgacgcc tgcgaccagg
     1561 tgtcggtcaa gctggcgatg gcggcctggg cgcgcgagcg cggcctgtgc tttgtcaccg
     1621 tcggtgccgc cggcggcaag cgacatggtg aacgggtcga ggtggccgac ctcgccgagg
     1681 tcacgcacga tccgctgctg gcccagctgc gctaccgcct gcgccgtcag cacggcgcgc
     1741 ccacgggcgg caagcgcctc ggggtgacgg cggtgttcag tcgcgagagc gtgcgcctgc
     1801 ccgaggcctg cgaggcgccc cagcaggtcg acagctcact gaactgccac ggctacggct
     1861 ccagtgtcgc cgtcacggcc accttcggcc tgtgtgcggc cggctgggtg ctcgatcgac
     1921 tgtctcagga ggggcaaaaa atcaggctat aatctgcggc ttgccaagtg actgacgcaa
     1981 gtcggtcggt cggcatcggg ggtcgttagc tcagctggta gagcagcgga cttttaatcc
     2041 gttggtcgca ggttcgaatc ctgcacgacc caccaaattc aaaggctcgg ccgtcaggtc
     2101 gggccttttt ctttgtgcgc cgccggctca atctctttgt gcgccgtcgg ctcagcacta
     2161 ggctcagtcg gcctgcaccc agcgctgccg ccactgggcg gtccaggcgg caagcgcttc
     2221 gggcaccgag gtgccggcct tggggtccag tgccagcacc gctgcggcgc gttgcagcgt
     2281 ccgcagcggg tcggacacgt cgagcgcttg cgcaccatgc tgcttgctga gtttttcgcc
     2341 gtcggcggcc agcaccagcg gtgtgtgcag gtagcgtggt gtggccaggc ccagcctgcg
     2401 ctgcagcagg atctggcgcg gcgtgttgtc ggccaggtcg gcgccgcgca ccacgtgggt
     2461 cacgccctgg gcggcgtcgt ccaccaccac cgccagctga taggcccaca ggccgtcggc
     2521 gcggcgcagc acgaagtcgc cgacctcatg cgccacatcc tgccgttgcg gccccaggcg
     2581 ccggtcgtgc cagacaatgg cctcgttggt tgcttcacta acgatagctg ctggcgcttg
     2641 cccggattgc cctggaggtg gatttgattc atgaactccg gtgtgcagcc gccaggcccg
     2701 cgcaggcttg ccttgcagtc cgccgcgctc ggggcggcag ctgccggggt agggcagctc
     2761 caccccgcgc tggcgcctga ggccgcgcgc ggcctgcgcc tgctcgatgt ccttgcgtga
     2821 acagccgcag gggtaggccg cgccggcggt gaccagcgcg tcgagcgcgg cctggtagtg
     2881 cgcgctgcgc ccggattgcc acaccggcgg ttcatcggga ggtaacccac aggcggccag
     2941 ctgctgcagc atctgccgat cggcgccggg cacgcagcgc ggcgtgtcca cgtcctcgat
     3001 acgcaccagc cagcgcccgt cgtgggcgcg tgcgtccagc cagctggcca gcgccgccac
     3061 cagcgagccg gcgtgcagtg gtccggtggg cgaaggggcg aagcggccga cgtacatggc
     3121 ggcgtagggc gtcggggctc ggcgtcgatc ggcccgttca ggcggcgatc acggcaagcg
     3181 cgagctccag cccggaaacg aaggcgtctt cgacccggct gccgaggcac cagtcaccac
     3241 acaggccgat gctgcctgcg ccgtcccaca ggtgcgtgcg gcccagcgac gccagggtgc
     3301 gcgcctggcg ccacagcagg gcttcggtgt gcgccggcgt ggcatggatg ccggtgattt
     3361 cagcaaaggc gcgcagcagc ttggcgctga cgcgcgccgg gtcgtcgcgc tcgtgttcgc
     3421 gtgaccaggc ggcgctggcc tgcaccgtcc agcgctcgac cgccacgcgc ccgggcttgg
     3481 acgattcgcg cgccagccaa ctgacgcggt ggtgcgtgct gcgcgccgca ttccactgcg
     3541 ggcccagcgt gtgcagccct ggctgcgtgg cgttggggta ggccagcatc agggtccagc
     3601 acggcgccac gtcgaccgct cccagggcag cgaacggcgc tgtgctgagg gtcgaagcct
     3661 gcaacagctc gttcgcctgc tggggcggga tggccagcag aacctggtca aagccggcct
     3721 gcaccggatg gtcttcgccc gggccgtcgg tatgcagctg ccagcgccct ggcgagagcc
     3781 ggtcgtgcgc cacgcgccag acgcgggtgt ccaggtgcag cgcgccggcc gcaagcagtg
     3841 gctcggccca gcgctggggc aggcgatcca tggcgggcac ggccacccag tgagattcgg
     3901 gcgccggcgg ggcggcctcc accacgcggc cgcgttcgtc gagcacacgc acggcgttgg
     3961 cgctccagcg tcgtaccacg tcggatgccg tggcctgcaa cgcctgcatg aagcgttcgt
     4021 cgcgcacggt gaagtactgc accccggcgt cgaagccgcc gaaggcggtc tgcacggtgg
     4081 ccatgcgccc gcccggttgg ggagcggctt cgaacacggc gacctcgtgc ccggcctgtc
     4141 gcagggtgcg ggcacaggcc agtccggcaa tgccggcgcc aacgacggcg atgcggcgcg
     4201 agaggtgggg gcgagggggc gtcatgcggc aactctacac gctcgggcgg ggttcgatcc
     4261 gggcgcgctc cggcgtgctg agccgccagc tgcgacaccg tgccagcccg gccgccgcct
     4321 cggaccaccc gtgcgcggca ggccgcccgt gccttgcccg acaatgcggg gcatgttcgt
     4381 tcatctgcgc ctgcacaccg agttctccgt cgtcgatggc acgactcgcc tggacgacat
     4441 cgtccaggcc gctgccgacg acggtcagcc cgcactcgcc atcaccgacc tgggcaacct
     4501 gtttggcgcc atcaagttct acaaggccgc gcgcggcgct ggcgtcaagc ccatcatcgg
     4561 tgccgaggtg ctgctggaag ggcaggggtc ggacccggaa gcgcccaccc gcgtgctgct
     4621 gctggtgacc agcgggcagg gctacctgaa cctgtcggag ctgctggcgc gtgcctggac
     4681 ccagggcgtg gtcaaggggc aggcggtggt gcgctgggcc tggctgggcg agctggccga
     4741 cggcctgatc ctgctgtcag gcgcgcaggc gggcccggtc gggcaggccc tggcgcaggg
     4801 cggtgtggaa caggccagtg cgctggccct gcaactggcc gagctgttcc cccaccgctt
     4861 ctacctggag ctgcagcgcg cagggcgcgc cgacgacgag gcgcatgtgg ccgcggccgt
     4921 gcagctggcc gcccgcttga agttgcccgt ggtggccacg cacccggtgc agttcgcccg
     4981 tgccgacgac tacgaggcgc acgaagccag ggtgtgtatc gccgagggtg agattctggg
     5041 caacccgcgg cgcgtgcgcc gcttcacgcc ggagcagtat ttcaagtcca cggccgagat
     5101 gcagcaactg ttcgccgacc tgcccgcggc gctgagcaac gcggccgaaa tcgcgcgccg
     5161 ctgcaacctc acgttggcgc tgggcaagcc ccgtctgccg gatttcccgg tgccgcaagg
     5221 gcacacggtg gaaacctatt tccgccagac ttccactgac gggctggagc agcgcctggc
     5281 gcacctgtac cccgacccgt cgcagcgtga cgccgagcga ccgcgctacc tgcaacggct
     5341 ggagttcgag atcgacacca tcctgaagat gggctttccg ggctacttcc tgatcgtggg
     5401 cgacttcatc aactgggcca agaaccatgg ctgcccggtg gggccgggtc gtggatcggg
     5461 tgccggctcg ctggtggcgt acgcgctgaa gatcaccgac ctcgatccgc tgcagtacaa
     5521 gctgctgttc gagcggttcc tgaaccccga acgggtgtcg atgcccgact tcgacatcga
     5581 cttctgtcag gccaaccgcg accgcgtgat cgattacgtc aaggacaagt acggccggga
     5641 cgcagtgagt cagatcgcca cctttggcac catggcggcg cgcgccgcga tccgcgacgt
     5701 cggacgtgtg ctcgacatga gctacacctt ctgcgacggc atcagcaagc tgatccccaa
     5761 caagcccggc cagcacatca ccatcgatgg cgccatgaag gtcgagccct tgctggccga
     5821 gcgctaccgc aaggaagacg aggtgcgcac gctgatcgag ctggcccaga agctggaggg
     5881 catgacgcgc aacgtcggca tgcacgccgg cggcgtgctg atcgcgccgg gcaagctgac
     5941 cgacttctgc ccgctctacc agcagcccgg cagcgaggcg gcggtcagcc aatacgacaa
     6001 ggacgacgtg gaggcggtcg gactggtgaa gttcgacttc ctggggctgg ccacgctgac
     6061 cattctggaa atcgccaagg agctgatcgt cgaacggcat ccgggtcagc agcacttcgc
     6121 ctacgaaaac ctgccgctcg acgatgcgcc aacctaccag ctgttcgctc aggggcgcac
     6181 cgaagcggtg ttccagtttg aaagccgcgg catgcagggc atgctgcgcg acgcgcgacc
     6241 gacgcggctg gaagacctga tcgcgctcaa cgccctgtac cgtccggggc cgatggacct
     6301 gatccccagc ttcgtggcgc gcaagcacgg gcgcgaggaa gtggtgtatc cgcacccgct
     6361 ggtggccgac atgttgtcgg agacctacgg catcatggtc taccaggagc aggtcatgca
     6421 gaccgcgcag atcctgggcg gctacagcct gggcggtgcc gacctgctgc gccgtgccat
     6481 gggcaagaag aagcccgaag agatggcgca gcaccgccag atcttccgcg agggcgcggc
     6541 caggaacggt atttccgagg gcaaggccga cgagatcttc gacctgatgg agaagttcgc
     6601 tggctacggt ttcaacaagt ctcacgccgc cgcctattcg ctgctggcct accacacggc
     6661 ttggctgaag gtgcactaca cggccgagtt ctactgtgcc aacatgaccg tggagatgga
     6721 caacaccgac aagctgaagg tgttgtacga ggacgcccag cgcgagggca tgcgctttga
     6781 gtctccggac atcaaccgtg gcggctatcg cttcgagccg gtcagcaaca ccctgattcg
     6841 ctacgggctg ggcgccatca agggcacggg ccagcaggcc atcgaagcca tcgtcgccgc
     6901 acgagaaggg cgcggcgagg gccccgccgg tgcagaacgc ggaccattca ccagcctgtt
     6961 cgatttctgc cgccgggtcg accgcacgcg cgtcaacaag cgcacggtgg aggcgctgat
     7021 tcgcgccggg gcattcgatg ccctgcagct gaaccgcgcg gcgctggcgg cctcgatcga
     7081 cctggcgttc gatttcgccg ccgccagtga agccaatgtc caccagggcg ggttgttcga
     7141 cctgatggaa gacggtcacg gctccagttc acaggagccc gaactgccgg cggtcacgcc
     7201 ctggggtgtg aaggagcgcc tgtcgctgga gaagacggcc atcggcttct acctgtcggg
     7261 ccatctgttc gacgaggtgg agcgcgaggt gcggcagttc gcgcgccgtt ccatcgccga
     7321 actggccgac agccgcgaac cgcagttgct ggccggcatc gtgggcgatc tgcgcatcat
     7381 caacggacag cgtggcaagc tggcgctgtt tcgcctggac gacaagtcgg gcgtgatcga
     7441 ggcgcgggcc gaggaagcgc tgatccatcg acaccgtcac atcctgaagg acgacgaatt
     7501 catcgtcgtc atgggcaagg ccatgcccga ccgcttctcc ggcggtctgc agttgaacgt
     7561 cacccagatc tgggatctgg cccaggcgcg ctgccggttc ggcaagtacc tgcgcgtggc
     7621 ggtggccggc aaggcacccg aggtgcgccg cctgctggcc gagtacccgc cccaggtgga
     7681 gcagaccgag cacggcgagc tgctgcgtgg tctgcccgtg cgcctgctgc tggagcggcg
     7741 cggctcggaa ggtggcgtca tggcccagtt gcacctgggt gagcaggcgc aattctttcc
     7801 cagcgatgcg gcgctggcca gctggacggc gcaggccgac gaaggccggg cgatggtggt
     7861 gtacgaatag cgcggcgcgc ttgccggagc gtcgtgttcg ccggcgcatg aaaaaaaccc
     7921 ggcctgagtc gggccgggtt tcgtgcgcgg gggcgtctgc ggcgccgtcg cctcagaagg
     7981 cgggggtgcg ttccgcgtgc ccgagggtcg cgcccccaag aggccgatcc agacgccaag
     8041 cccagccgca gcctgagccg cggccagtgt gagccgcgac cctcgggtgg gtagcgtggt
     8101 cacccaacag gtgaaggcaa cgccatcgga gccgcgaaac cccgcatcga tgcggcccgc
     8161 ccgaagatga caagcggtca actgccgctt tgagttgtca ctcgaggtgg acttcgacgc
     8221 ggcgcgcctc ggcgttgctg ccgctggcgg tggtgacggc gggtttcctc aggtcgatct
     8281 tgtcctcgcc gatgccgagt gcggccagcg cgtcgcggac cgccaacgca cgctgcttgg
     8341 ccagctcctc gttcctgacc gggtcgccgg tgacgtcgtg aaagccgctg atcaccgcct
     8401 tcttgccggc ggccacgcct ttgacgacct cgcccagcgc ttcggcagca cctgccgcga
     8461 gttcggcgct gccgctggcg aagtagaact tgaccacgcc gttctcgacg cggatcgctg
     8521 cgccatcgcc cacctcgcca ctggtcatgg ccgcggtgtt ggccggagca cccgccgcac
     8581 cgccggcagc cgtgccgcct gcattggcgt tggcgttggc cgcgctggag gcggccgcgg
     8641 gagccacgct cttgcccgtg cggtacagcc ccatgccgac ggccaggccg atggccagca
     8701 gcaccacacc gatcagcacg gcccagacga cgccgcgctc ttcttccctt tgttcactca
     8761 ttgatgggct ccagaaaaat ggcgtccatt gtataaattc ctgccatgcg ctccgccgac
     8821 gcaccgctgc cgcctgctta cgccgacttt gcgcacctgc gcgcccctgg gcgcgatccg
     8881 ctgcggctgc tggagcacac gcgtgcccag tggcaccgtc gcgccggtcc gcaagccgat
     8941 ctgtgggtgt tcggctatgc ctcgctgatc tggcggccgg acttcgactt cagtgagcgc
     9001 catgccaccc gcgtgcatgg ctggcaccgc gcgctggcca tgtggagccg cgtcaaccgc
     9061 ggcacgcccg agcaaccggg gctggtgttc gcgctgttgc cgggcggaag ctgccagggc
     9121 atggtgtttc gggtgccgcg cggcacggcc gatgcggtgt tcgacacgct ttgggcgcgc
     9181 gagatgccca gcggcgtgta cgacgcgcgc tggctgccct gtgccacgcc gcacggcatc
     9241 gtgtgggcgc tggcgttcac cctgtcgcgc cgcagtccct ccttcacggg ggcgctgacg
     9301 ccagagcagt atcgccacat cttccgccat gcccgcgggc gctacggcac cacgctggac
     9361 tatgcccgtc gcacgctgga aaccctgggc gcccatggca tccacgatcg ccgcctgcgc
     9421 acgctgctga gcctggtcga cgatgaagag gcgtgagcgg gggtgaacgt ccctgatgcc
     9481 cgctcggacc tcccggaaca cacgcgggag gtggccggtg ctgcggctgc gcctggcgcc
     9541 tggatcgggc gcgccgggca cgcggcatgc gcttcgagct tgcgggacgg gacggcgcat
     9601 gatctgccta tactgccgcg gatgaaagac atcgctcatc tgcgcaagag ttatgagcgt
     9661 gccgagctgt ccgaggacgc ctcgcacgcc gatccgggtc ggcagtttgc ccagtggttc
     9721 gaggaggcac gccagagcga gctgcccgag cccaatgcca tgacgctggc caccgtgggc
     9781 agcgacttgc ggccttccac gcgcgtggtg ctcatcaagg aattcgatga acgcggcatc
     9841 gtctggtaca ccaactacga aagccgcaag gggcgtcagc tggccggcaa cccgtacgcc
     9901 gcgctgcagt ttcactgggt cgaactggaa cgggtggtgc gcatcgaagg cctggtcgag
     9961 caggtgagcg aggctgaaag cgatgcctac tacgccagcc gcccgttgga ccaccgcctg
    10021 ggggcctggg ccagcccgca aagccaggtc attcccggac gtgcctggct ggctgccgag
    10081 gcggccaagg tcggcgccag gtttttgctc aacccgccca gaccgccgca ttggggtggc
    10141 tttcgcctca ggccggaccg ctgggagttc tggcaaggcc gcaagagccg cctgcacgac
    10201 cgtctgtgct accgactgga agcgggcggc tgggtgcgcg aacgcctggc gccctgatgc
    10261 ccgaacgtgc ggcagcgacc cgcccggtct cggctgcgcc agggcggccg atggccggtt
    10321 ggggtggcga tctcgctgcg cttgacccgg atcggcagcc gcgtcgggtg cgagcagccg
    10381 gcctctggca agatgggggg tcttcaccgt tgggcccttg ccaggatgct tccatgcccc
    10441 cgcctgcagc gatcctgatc cgacgctttc gcgaacccgc gtttgcgcag cgtttcgccg
    10501 ccgcgcggcc tggtgccgcc gggtgccggc agcgtgtcgt tgcggcaaag gcattgcttg
    10561 tcatgccgca gcgcaggccg gggccgcttc ggcatggtgt gggcatcttg cacccagtcc
    10621 acttgcatag cgtgaacagg tcctaggcca tggagcccgt tcgcctcatg cgcgagatgt
    10681 tcgaggccgc catcgcctcg gcgcaaccgg cgcgctgcgt gcctgctcac ctgccgaacc
    10741 cggctgaagt gcggggtcga ctgatcgtgg tgggcgccgg caaggcgtcg gccgcgatgg
    10801 cgcgcgcggt ggaagagcac tggcgcggcc cgctcgatgg cctggtggtg acgcgctacg
    10861 gctaccacgt gccgtgtcag cacatcagga tcgtcgaagc ggcgcacccg gtgcccgatg
    10921 ccgccgggct gcaggcggcc gagcgcatgc tcggcctcgt ctcgggcctg accccggacg
    10981 atgtggtgct gtgcctgatc tccgggggcg gctcgtcctt gctgcccttg cccctgcccg
    11041 gcatcacgct ggccgacaaa cagcaggtca accgtgccct gctggccagc ggcgccagca
    11101 ttggcgagat gaacaccgtg cgccggcatc tgtcggccat caagggcggg cgcctggccg
    11161 cggcctgcca cccggcgcgc gtgatcacgc tggtgctgtc ggacgtgccg ggcgacgcgc
    11221 cgatggacat cgcctccggt cccacggtag gcgatcccac cacctgcgcc gatgcgctcg
    11281 acatcgtgca gcgttacggc atcgagttgc ccgcgccggt gcgtgtgctg ctggacagcg
    11341 ggcgcggcga gtcggtcaag cccgacgatg cgcgactggc gggccacacc acgcgcatgg
    11401 tcgcggctcc gcagcaggcc ctggaggtgg cggcgcaggt ggcacggcgg catggctacg
    11461 ccgcgcacat cctgagcgac gccgtcgaag gcgaggcgcg cgaggtcggc aaggtgctgg
    11521 cggcgctggc gctgcaggtg gccgggcgtg gccagccctt cacggcccct tgcgccatcc
    11581 tgtcgggcgg cgagaccacc gtcacactgc gcggtcaggg gcgcggcgga cgcaacgtcg
    11641 agtgcctgct gtcgctggcc atcgccacgc gcggtcacgc gcgcatccac gccctgatgg
    11701 gcgacaccga tggcgtggac ggcgcgcagg aggtcgccgg cgcgcacatc ggcccggaca
    11761 ccctggcacg cgccttcgcg caaggcatcc atccgcgccg cagcctggac gacaacgacg
    11821 cccatggctt ctttcaggcg ctgggcgatg cggtggtcac cgggcccacc ctgaccaacg
    11881 tcaacgactt tcgagtcatc ctggtcaacg cctgaccggc cgcggtgcag cgccgccggc
    11941 gaacccggcg cccacctgcc cagacacggg ctggcggcgt gcggttgctg aactgggccg
    12001 atgtttcatg cgcagaatat gccttcagcg ctcacctggc aagcgcgagc agctatcgta
    12061 atcatagtat ttgacacgca ggctccgcag gttctttgcg ctccctcgtc ctgctggcgt
    12121 gggcaatgcg ccgataatgc gggtttcccc gccgcccgat cggtcgcccc gcaccgaacg
    12181 tgaccgccat gaacgctccc ctgtcttccc ccgctgctgc ttcggccatc tcgccggtgc
    12241 ccgacatcgt ggccgagctt gccgccggcc gcatggtgat tctggtcgac gaagaggatc
    12301 gcgaaaacga aggcgacctg atcctggcgg ccgaccatgt cacgcccgag gcgatcaact
    12361 tcatggcgcg cttcggtcgc ggcctgatct gcctgaccct cacgcgcgag cgttgcgagc
    12421 gcctgcgcct gccgcccatg gtgacgcgca acggcaccaa gatgggcacc gccttcacgg
    12481 tctccatcga agccgccgaa ggcgtcacca ccggcatcag cgccgccgac cgggcgcgca
    12541 ccgtgcaggc cgcggtggcg gcccaggccc gcgccgacga cctggtgcag cccggccaca
    12601 tcttcccgct gcaggccgtc gacggcggcg tgctgatgcg cgccggccac accgaggccg
    12661 gctgcgatct ggccgccatg gccggctgta cgccggcggc ggtgatctgc gagatcatga
    12721 aggacgacgg caccatggcg cgcctgcccg acctgcaggt gtttgccgcc gagcacgggc
    12781 tgaagatcgg caccatcgcc gatttgatcg agtaccgcag ccgcaccgaa tcgctgatcg
    12841 aaaaaatcgc ctcgcgcccg ctgcgcaccg cacatggcga attcatcgcc cacgcctacc
    12901 gagaccagcc cagcggcgcg ctgcatctgg gtctggtgct ggggcagtgg cagcccggcg
    12961 ataccgtgcc ggtgcgcgtg cacgagccgc tgtcggtgct ggatgcgctg gaggtgcagc
    13021 gcggcagcca ttcctggagc gtgcacgaaa gcctgcagca catccagagc gccgggcgcg
    13081 gcgtggccgt gttcctcaac tgcggcgaag acgccggtca gctgctggcg cagttccagg
    13141 gcacggcgcg cgccgcccac gcacctgagc gcgggcgcat ggacctgcgc agctatggcg
    13201 tgggcgcgca gatcctgcgc gacctgggcg tgcagcgcat gcagctgctg ggcacgccgc
    13261 ggcgcctgcc cagcatggcg ggcggctacg gcctggaagt gaccggctac atcacgaagg
    13321 attgagaagg acgcccatgc ttggtgccga caaaggacag actctttcac tcgacggcag
    13381 cgcgctgcgc atcggcatcg tgcaggcgcg cttcaacgaa ggcgtgaccc aggccctggc
    13441 cgacgcctgc ctgggcgagc tggccgcatt gggtgtgccg gcggcccaga tcgtccacca
    13501 caccgtgccg ggtgcgctcg aagtgccgct ggccttgcag tggctggcgc ggcgcggcga
    13561 atgccatgcg ctgatcgcgc tgggctgcat cattcgcggc gagacctacc atttcgaact
    13621 ggtcgccaac gagtccgggg ccggcgtcac gcgcgtggcg ctggacctcg acatcccggt
    13681 ggccaacgcc atcctgacca ccgagaacct ggcccaggcc caggcgcggc agaccgacaa
    13741 agggcgcgat gcggcccggg tggcggtcga aatggcgctg ctgaaggcgg gcctgcgatg
    13801 agcccggcac ccaccacgcc gcgcgcggcc ggcgccgcgc cgcgccccgg cgaaggcggc
    13861 acgcgcaagt cttcggtcaa atcgccgcgt tcgcgcgcgc gcgaatttgc cctgcagggc
    13921 ctgtaccagc acctggtcgg cggtggcgac gcggccgcga tcgacgtctt cacgcgcgag
    13981 ctgcaaggct ttgccaaggc cgatgcggcg catttcgacg ccctgttgca cggctgcatc
    14041 gagctggccg acgacctcga cgcgctgatc gtgccgctgc tggaccgccg gctggccgac
    14101 atctcgccca tcgagcgcgg ctgcatgtgg atcggtgcct acgaattcct caagtgcccc
    14161 gacgtgccct ggcgcgtggt gctcaacgag tgcatcgagc tggccaagga tttcggcggc
    14221 accgacggcc acaaatacgt caacgccgtg ctcaacggcc tggcgccgca gctgcgcgcg
    14281 cccgaggtgg ctgccgaccg cgcccgggtg cgcgccgcca agggccggga cgcgacatga
    14341 ggatctctgc ccgcgcccag cgcatcgagc cgttctacgt gatggagatg gccaagtcgg
    14401 ccgccgccat cgcccgcgaa gccgcgcacg gcgatcggcc gatgatctac ctgaacatcg
    14461 gcgagcccga tttcaccgcg ccgcctctgg tgcaggcggc ggccgagcgg gccatccgcg
    14521 acggtcacac ccagtacacc caggccaccg ggctgccgct gctgcgcgag cgcatcagcc
    14581 agtggtacgc cgaccgcttc gggctgcggg tgccggcgcg tcgcatcgtc gtcaccgccg
    14641 gcgcctcggc ggcgctgcag ctggcctgcc tggcgctgat cgaggcgggc gacgagatcc
    14701 tgatgcccga tcccagctac ccctgcaacc gccagttcgt cagcatggcc gagggccgtg
    14761 cggtgctgct gccgtccacc gcggccgagc gctttcagct cagtgccgac aaggtgcgcc
    14821 aggcctgggg cgcacacacg cgcggcgtca tgctggcgtc gccctccaac cccaccggca
    14881 cctcgatcca ccccgacgag ctggcgcgca tccacgcggt ggtgagcgag cgcggtggca
    14941 tcaccctgat cgacgagatc tacctcggtc tgtcctacga cgacacctac ggccacagcg
    15001 cgctggcgct ggacgaccag atcatcagca tcaacagctt cagcaagtac ttcaacatga
    15061 ccggctggcg cctgggctgg ctggtggtgc ccgatgcgct ggtcgaagtg gtcgagcaga
    15121 tcgcccagca cctgttcatc tgcgccagca ccgtgtcgca gcacgctgcg ctggcctgct
    15181 tcgagcccga cagcctggcc gagtacgagc gccggcgcgc cgaattcagg gcgcggcgtg
    15241 actggttcgt gccgcagctg ggcgccctgg gcctgacggt gccggtgatg cccgacggcg
    15301 ccttctacgc ctgggccgat tgctccgccg cctgtgacag gatgttcgcc ggccgctccg
    15361 aatacaatag cgagatgcag catccgggca gttgggaatt cgcctttgaa ctgatgaagc
    15421 gcgcgcacct ggcggtcacg ccggggcgcg acttcgggca tgccgagacc gcgcgctgcg
    15481 tgcgcttttc caccgccaac tcgatggcgc agctgcacac cgccatcgag cggctgaagg
    15541 cggtgctggc ctgaggcggt ggtgcatggc ggaattcgag tttccgatcc gcgtctactg
    15601 ggaagacacc gatgccggcg gcatcgtgtt ctacgccaac tacctgaagt tcttcgagcg
    15661 cgcacgcacc gaatggctgc gtgccggggg cgttcaccag cgcagcctgc gcgagcaggt
    15721 gggcggcatg ttcgtggtgg tcgagtcgca ggtgaaatac ctgcgcccgg cgcggctgga
    15781 cgatgaactt gtggtcaccg cgcggctcca agaggccggt cgggcgggcc tggtgatcga
    15841 tcagcaggcc ttcgtgcgac gccaggcgcc cgagccacgc gagctgctgt gccgtgcccg
    15901 catccgtgtg ggctgggtcg atgccgtcac cctgaaaccc gctcgcattc cgcccgacat
    15961 tctgaaaacg ctgcctacat gaaccaggac ctttccatca ttcaactggt gctgcacgcc
    16021 agcttcgtgg tgcagctggt gatgctgctg ctgctggtgg tgtcgatcac cagctgggcc
    16081 gccatcatcc gcaagctgcg ctcgttcaag cagacgcggg cgctcaacga cgagttcgag
    16141 cgcgacttct ggtccggcac cagtctgaac gacctgtacg cctcggcggt gcagaacgcc
    16201 aaacacgccg ggccgatgga gcgcatcttc gccagcggca tgcgcgagta ccagaagctg
    16261 cgcgagcgcc gcatcggcga tcccagcgcg ctgctcgacg gggcgcgccg cgccatgcgc
    16321 gccagccagc agcgcgaact ggacgaggcc gaatccaacc tgtcgttcct ggcctcggtg
    16381 gcctcggtca gtccctacgt cggtctgttc ggcaccgtgt ggggcatcat gcacgccttc
    16441 accggcctgg ccagcctgca gcaggtgacg ctggccaccg tggcgccggg cattgccgag
    16501 gccctggtgg ccaccgccat cggcctgttc gccgccatcc cggcggtggt ggcctacaac
    16561 cgcttcgcgc gcgagctcga tcgcctggcg atcaagctgg agaccttcat cgaggagttc
    16621 tccaacatcc tgcagcgcaa cctgggtgtg catgcccagg cggcgcattc caccaccccc
    16681 accggatact gaagccgtgc ccgccgtcag ctcccgcggc cgaggccgcc gcgccatgaa
    16741 cgagatcaac atggtgccgt tcatcgacgt gatgctggtg ctgctgatca tcttcatggt
    16801 gacggcgccg ctgatcacgc cgggcgtcat caacgtgccc agcgccggcc agtcgagcaa
    16861 ggcgcccgac caacgggtgg acgtgcagat cgacgccgat ggcaagctct cgctggccgg
    16921 ccagattcag cgcagcggcc tgggagacaa ggaggcggtg gccgccatcc gggcgctgct
    16981 ggccgacaac ccggagttgc cggtgatgat cgctgccgac agcgagctgc gctaccagca
    17041 ggtgatgaac atgatgaagc tgctgcgcga agctggtgtg cagcgcgtcg gtctggccgt
    17101 caagtgaggg ccgcgccatg aatgtcgccg ccatcgaacg cgccgagttc gcgccgccgc
    17161 cgcagggcgg cttcggacgc gccttcgggc tggcgctggc ggcgcacctg ctgctgatcc
    17221 tggcactgac ctggggcctg cgctggaaga acgaaccgca ggatctggcg gtgcaggccg
    17281 agctgtggtc gcccacggtc cagcgggctg cgcccaaggc ggcgccgcgc gcgcctgcgc
    17341 cgcaggtcga gccgccgcca cctcctgcgg cgctgccgca gcccaggccc gagccggcgc
    17401 tcaagccgcc gccccctcag gtggacgagc aggcgcagcg cgacgccgac atcgcgctgg
    17461 cgcgcaagaa gcaggaagag gcccgccagg ccgccgccca gcgcgaacag gcaaggcgcg
    17521 aacgcgagaa aaaggagcaa gccgagcagg agcgccgcga gctggcccag aagaaggccg
    17581 ccgaagagca aaagcgcaag caggacgagc tgcgcaagct ggccgcgaag aaacaggccg
    17641 aagaagccga acagcgccgg caggccgctg ccgaggcaaa gaagaaggcc gaggcgcaaa
    17701 agcgcgccga ggcggaggcc gaacgcaagg ccgaagcgca ggccaaggcc aaggcggcgg
    17761 cccaggccag ggcggccgag gacgcacggc acaaggccaa cgtggcgcgc tcgctggcca
    17821 tggccggcag cgccgacagc gagggcggca gcgccgccgg caagggccgg ggcggcggca
    17881 gcgccggggg tcaggatgag cgcgacgccg gtccctcggg caactacgcg ggtcgcatcc
    17941 gcgccgcggt gcgacccaac atcgtgttca ccgacgatgc gccgggcaac ccggtggccg
    18001 aggtcgaggt gcgcaccgac caggccggca acatcatcag ccgccgcatc acgcgctcca
    18061 gcggcgtcag gacctgggac gacgcggtgc tgcgcgcgct cgaccgcacc gagaagctgc
    18121 cctcggacaa cgggcgctac tggaacccga tgctgatcac cttccggctg cgtgactgag
    18181 ccggagcagg gtggcgcttc agtttgctat taaaaacata gctgtcagcg caataccagc
    18241 aagcgccaga ggcaaaaaaa ccttgaaccc cgcggcggcg cctcagcgca gcccgccgcc
    18301 ggcggcgcct tcggcgcgct ggatcaactc gatcttgtag ccgtccggat cggtgacgaa
    18361 ggcgatcacc gtggtgcccc ccttgaccgg cccggcctcg cgcgtgacct ggccgccggc
    18421 ggcacggatg cgctcgcagg cggcgtaggc atcgggcacg ccgatggcga tgtggccgta
    18481 ggcggtgccc aggtcgtagc tgtcggtgcc ccagttgtag gtcagctcga tctccgcctg
    18541 tcccgggttg ccgccttcgt agccgacgaa cgccagcgag tagcggtact ccgggttctc
    18601 cgaggtgcgc agcagctgca tgccgagcac ctgggtgtag aagtcgatcg aacgttgcag
    18661 atcgccgaca cgaagcatgg tgtgcagaag tctcatgccg ccattgtgcc gtcggcggcc
    18721 ggatcgccgc tggccgggcg cccgccaccg ccacgggatg tgtcgaaccg agctcgcacc
    18781 ttcgatacac gacaacccgc acgatgaaac ctagacacgg ccgttggacg cgcccgccgg
    18841 tgccgtgatc ggtgtgccag gcaagacgca acgacgccgc gggctcctgc ccgcaagcag
    18901 gagcaacgcc gcatggcgcg gcgagcgcg
//
LOCUS       DAOQTZ010000051        18687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3427_length_18687_cov_13.066123, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000051 DAOQTZ010000000
VERSION     DAOQTZ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 18687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18687
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3427_length_18687_cov_13.066123"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /gene="wrbA"
                     /locus_tag="PKN51_07675"
     CDS             complement(<1..584)
                     /gene="wrbA"
                     /locus_tag="PKN51_07675"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNT85015.1"
                     /translation="MSVRLAIVYYSTYGTNHQMAEIAAEAARAAGAEVRLLKVPETAP
                     EAVVAGQPAWKAQADKTAQLPQATAADMEWANAYLIVAPTRFGIMASQMRAFIDTLGG
                     VWAKGGLANKAVSAMTSAQNAHGGQESTLLSFYATIMHWGGIVVAPGFTDPVIFKVGG
                     NPYGYSHTQGQPFSDDVKAAIGHQARRLVQFAAKL"
     gene            714..1667
                     /locus_tag="PKN51_07680"
     CDS             714..1667
                     /locus_tag="PKN51_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT85016.1"
                     /translation="MNPISLPANDLILFAHIVAAGSFTRAADRTGLPKATLSRRLSEL
                     EDALGERLLQRSTRRLALTEFGQHMLEHAQRLLEESAAATALAQHRQLTPQGTLRASF
                     PPEFSALSLVKVVSEFARRHPEVRLELDLSARRVDLVAERFDLAVRAATQLPDDSTLV
                     ARHIVTHEHGLFASPDYLRRHGTPATPAELERHVGLAMLGSGGERQPWRLTRGKERWE
                     GLPAQVHCANSVGLQQALAVQGLGIVGLSALFVRQSRDADSLQRVLPDWCLPPTGVWC
                     VTPGRRLLPKRTSAFIQILTEVLAEGGAAPAPLAAGHRPRR"
     gene            1744..2745
                     /locus_tag="PKN51_07685"
     CDS             1744..2745
                     /locus_tag="PKN51_07685"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNT85017.1"
                     /translation="MSAPTSPQRPAVLVARAIFPDIVEQLSRHFEVESNPDDTLWPRA
                     ELVRRLQGKQGVFTTGGERIDAELLAACPGLRICANMAVGYNNFDLPAMTAAGVLATN
                     APGVLTETTADMGFALLMAAARRVTEGEHFLRAGAWDRWAYDMLIGQDVHGSTLGIIG
                     MGRIGQAIARRGALGFGMRVIYHNRSRLPATDEAALGARYVSKQELLQSADHVVLVLP
                     YSAASHHTIGAAELALMKPSATLTNIARGGIVDDAALAAALREGRIAAAGLDVFEGEP
                     KVHPDLLACRNVVLTPHIGSATVPTRRAMAQLAADNLIGYLVHGKPLTPLNPEVAAL"
     gene            2912..4426
                     /gene="rmuC"
                     /locus_tag="PKN51_07690"
     CDS             2912..4426
                     /gene="rmuC"
                     /locus_tag="PKN51_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNT85018.1"
                     /translation="MDLSNPVWLPWLALAALLLNLLLLLALLLRRPRRPADVAARDEV
                     RQWLDQQGERLERGLRQEMGEGARSGRQELAQALASFQSAVTAQGAEAVRTQNAQVDA
                     LAMQLTQLRGTLGDTLVGQLQQLALTMTQQAQEATRTQNAQIDAFAQQLAHLRGSLSE
                     TLTQQLQQLSEANARRVQEMRATLEQQIGALQAANSAKLDEMRQTVDEKLHATLEQRL
                     GERFKQVAERLEQVHKGLGEMQTLAQGVGDLKHLLANVKTRGTFGEAQLGQLLEQVFA
                     PEQYAAQVATRPDTRHAVDFAIRLPGRGDDGAPLWLPIDAKFPTEDYERLLDAQQRAD
                     AGAAEAAGKALEARIRLEARSIADKYVEPPHTTDFAILFLPSEGLYAEVLRRPGLMEA
                     LQRELRVTLAGPTTLLAMLSSLQMGFRTLALEKRSSEVWQVLGAVKTEFAKFGDVLAR
                     VKSQTQTVLNTLDAAQTRTNVMNRALRRVEALPDEQALQLLPGGGDADGAGGNS"
     gene            4497..5687
                     /locus_tag="PKN51_07695"
     CDS             4497..5687
                     /locus_tag="PKN51_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT85019.1"
                     /translation="MALSGRQVLRLMLAQVCVHSAMTGTRLAAPLLALKLGYSAAAVG
                     VLLALFALSAVFLALPAGRLADRHGLHLPLALAVSAAMLGAGLAAAWPVFAVLCASAL
                     LVGAAANAAQIALQRHIGRLASDAAERKLAFSWIAIAPAAANFAGPLVAGLLIDRAGP
                     QAGDLIGYRWAFGLMAALPLLCWALLRGTPRAPVVPAAAGEERASVWDLLGHAAMRRL
                     LLVNWLQSMAWDVHMFVLPLLGHERGLSASVIGALQGAFAVAAALVRMALPLLARRLA
                     EWQVIYASTLLAACALLAYPWMPGPLGMGACSMVLGVALGAVQPMVMSLLHEVAPIER
                     QGEAMALRSLTMNASSFTMPMLFGSIGAVIGTGGLFWLVALVLALGARSVPALGGAAR
                     PPSA"
     gene            complement(5758..6216)
                     /locus_tag="PKN51_07700"
     CDS             complement(5758..6216)
                     /locus_tag="PKN51_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNT85020.1"
                     /translation="MTHPAEQLKALGISLPPVSTPAAAYVPFVRSGPLVFLSGHIARR
                     DGQPWVGKLGASMTTAEGAQAARAVAIDLMGTLQAAVGDLGKVRRIVKVMSLVNSTPD
                     FTEQHLVTNGCSELLGQVFGDAGKHARSAFGVAQIPLGACVEIELIAEVD"
     gene            6445..8916
                     /locus_tag="PKN51_07705"
     CDS             6445..8916
                     /locus_tag="PKN51_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNT85021.1"
                     /translation="MRFSEPPPNPAALGRWRGHALTALLAGGVLGVAAQLQQARLWPA
                     WAYALTGLLALLLAVVAWRPRRAGARALGLWLLAGALAAGASTGWRAGLRQAERLAPA
                     LEGRDLRLTGRIAGLPQRGEAGWRFRFDVERASLDGAPVRVPPRVQLGWYGWVATVDE
                     TDAERPVAAALRSGDRWQLTARLKAPHGNLNPHGFDLELWLWEQGVGASGYVRSGPRE
                     APARRLEEGGASLPRWRQAVRDRILDGADAGDATARRLAGIVAALVTGDQNAIERADW
                     DVFRATGVAHLMSISGLHITMFAWLAAALVGALWRRSARWGWPARANPCLWLPAPTAA
                     RVGGVLLAAGYALFSGWGVPAQRTVLMLATLTLLHVAGLRWPWWLNALAAAVVVLALD
                     PWALMQPGFWLSFVAVAVLFATDSRAAGAQQTGARARWLALLREQGVVTLALTPLSLI
                     LFGQASVVGLLANLLAIPWVTLLVTPLALLGVLWAPLWALAGAALWPLAALLGWLAAW
                     PWASVSLPAAPWWLGVAAVAGGLLLALRWPPAWRLAGLPLVLPLALWQPARPAPGQFE
                     LLAADVGQGSAVLVRTARHSLLYDAGPRYSLESDAGHRVLVPLLRALGERLDSVVLSH
                     RDSDHTGGARAVLAAQPQAELRASIEDGHELQALRPATRCQAGQRWQWDGVAFELLHP
                     DAADHARPLKSNALSCVLRVRAVDGAAALLTGDIEQAQEQALVARTPELRADVLLAPH
                     HGSRGSSSTALLAAVRPRWVLVQAGYRNRFGHPAAEALARYAAVGAQPVGSPRCGAMR
                     WHSAQPGALRCERDTAARYWHHQPP"
     gene            8913..9404
                     /locus_tag="PKN51_07710"
     CDS             8913..9404
                     /locus_tag="PKN51_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNT85022.1"
                     /translation="MKTLLIVYHSLTGGTRQMAEAAAQAARQEGGVAVRLLTAAQAGP
                     PDLLAADGYLFATPENLAAMAGLMKDFFDRCYYPVLERIQGRPYAAMICAGSDGAGAL
                     RQLERIATGWRLKQVAPGLIVCTHAQTPERILAPKTIEPPDLARCAELGAGLAAGLGA
                     GVF"
     gene            complement(9426..10511)
                     /gene="mltB"
                     /locus_tag="PKN51_07715"
     CDS             complement(9426..10511)
                     /gene="mltB"
                     /locus_tag="PKN51_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNT85023.1"
                     /translation="MTAPRLPFDRRRLLATICIAAGALPVSAAAQNGPKRRRLPEAAP
                     SSYASRPDALRWAEDMAARRDLPPDWVRDTIGQAQHLPNVPRLMLPPARGTAKNWRAY
                     RSRFIDATRIRAGARFWQRNRATLARAQQDYGVPPEIVVGIIGVETIYGQNMGSFRVL
                     DALATLSFDFPPQHPRAAARQAFFQDELAQYLSLCQRSGMDPQQPLGSYAGAMGMPQF
                     MPSSWVRYAIDFDADGHIDLWGSEGDAIGSVAHYFQGYGWQPGQPTHYPVQLAPEADL
                     DRLLAPDILPSFRPARFAELGARLDAAGQAHDGPLALIELQNGAAEPSYVAGTENFYV
                     ITRYNWSSYYAMAVIELGQEVAAALGA"
     gene            complement(10486..12546)
                     /locus_tag="PKN51_07720"
     CDS             complement(10486..12546)
                     /locus_tag="PKN51_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNT85024.1"
                     /translation="MTLTALRRLPREARDTLFLLAVIGWLILPLVPHLPLWCSALAAA
                     VLAWRGLLALRSRPLPRRAWLVLLLALTLVGTWTSHRTLLGRDAGVTLVVVLLALKTL
                     ELRARRDALVVFFLGFFTLLTNFFYAQTLPVATAMLIGLLGLLTALVHAHRPVGEPSL
                     RESAAVALRMALAGTPVMLALFLFFPRLAPLWGMPSDALTGRSGLSSTMEVGQVAKLA
                     LDDGIALRVKFDGPPPPQGQLYFRGPVLSRFDGRQWTARGGPGFEGLGLPPPSDLRVQ
                     GEPVRYEVTLEPSLRPWLLTLDVAPRAPELPEGRSARLTGDLQWLSPRPVSELLRYRA
                     ESYPLFHYGLVGSDGQARRDLDEQLRLPADHNPRTLALAQQLRDRHGAGNTLALVQAA
                     LERLRSGGYHYTLEPGVSGQHSADEFWFDTKAGFCEHISSAFVVLLRAAGVPARIVTG
                     FQGGELNGVDGYWVVRNADAHAWTEVWDPGRGWLRVDPTAAVQPARIGQLQRLRRPEG
                     LVAGALGTLSPTLMAQLRSLWEAANNGWNQWVLNYTQSRQLDLLRQLGFASPNWTDLG
                     KVLAGLLVAAALLGMAWARWERSQHDPWLRLLARARRLLAQAGLNAPPQAPPRALAQQ
                     VAHSTLPAALRQPLTDWLLALETLRYAPSQAAAPRLTTLQRELRRLPWPRHPTRHDRT
                     PPAL"
     gene            complement(12573..13496)
                     /locus_tag="PKN51_07725"
     CDS             complement(12573..13496)
                     /locus_tag="PKN51_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT85025.1"
                     /translation="MRAWWQSRLPLTDTLLLTQRNVYILPTRAGWMLAATLLVLLIAA
                     INYQLNLGYLLTFLLAGSAAAGMVVGHHTLRGLHLQLLPPQPLFAGALAVLDVKLTST
                     RRTPRHAIGVALLEGAHWSWADVPAQGQATVQVSFQPARRGLHRVPALTVETRFPLGN
                     FRVWTVWRPAAQVLVYPAPEPQAPPLPPGEPRAGSGVAAGARASGEFDGVRAYQRGDP
                     ARLIVWKKFARAEAPVSRDTLHLQRQTLWLDYARAGALDPEARLARLTAWVLAADAQQ
                     LDYGLRLPGREIAPAGGAAQRLRCLQALASA"
     gene            complement(13775..14695)
                     /locus_tag="PKN51_07730"
     CDS             complement(13775..14695)
                     /locus_tag="PKN51_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT85026.1"
                     /translation="MDVTSKIQAVLAQLNTVVVGKPTQVQDGVTCLLAGGHLLIEDVP
                     GVGKTTLAHALARTFGLQFSRVQFTADLMPSDLSGVSVYDRGKEAFVFHPGPVFAQVL
                     LADEINRASPKTQSALLEAMEEQQVSVEGETRPLPWPFFLIATQNPFDQLGTYALPES
                     QLDRFLMRISLGYPERAAERTLLAGHDRRELLDRLPALLDASALAALQQAVARVHVAE
                     PLLDYVQDLIAATRSGRWFMQGLSPRAGIALVRAARARALIAGRDYVAPDDVQAILPQ
                     CVAHRMVPVSQSGRGALEQVRAMIDAVPLA"
     gene            14749..15702
                     /locus_tag="PKN51_07735"
     CDS             14749..15702
                     /locus_tag="PKN51_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNT85027.1"
                     /translation="MSRTGYFTHPDCRKHDMGAGHPECPQRLDAIEDRLLITGVADAL
                     ERREATPAAMGDLLLAHGRRHVAALRGLNDILLEEMDAGGPDRYSLDPDTSMNRYTYR
                     AALLSAGAAIDAVDAVVAGELTNAFCATRPPGHHATRNQAMGFCFFSNVAVAAKYACE
                     RHGLKRVAIVDFDVHHGNGTEDIVAGDERILMVSFFQHPFYPEGGAQKQDANLVNCPV
                     PAYTRGMDVRELVELMWMPRLEQFQPELVFISAGFDAHREDDMGQLGLTESDFAWITE
                     RVKGVAERFAQGRIVSCLEGGYNLNALARSVEAHLRVLAEV"
     gene            15823..16632
                     /locus_tag="PKN51_07740"
     CDS             15823..16632
                     /locus_tag="PKN51_07740"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNT85028.1"
                     /translation="MSASPAIPAEPLVRHQRDARGVHRLTLNQPKSFNVLSEAMLAEL
                     QRLLDQIGADPAARAVVIGAEGKAFCAGHDLKQMKASPRLDYYQALFAQCSRMMLSIL
                     RLPVPVLARVQGLATAAGCQLVAQCDLAVAAQGARFGVNGIDVGLFCSTPSVPLVRNI
                     APKQAMEMLLTGGFIGAEEAQARGLVNRVVPPEQLDETVEALVGAILAKPRAAIAAGK
                     ELFYRQRELGIEAAYQLAGQTMACNMMLDVTQEGVQAFIDKREPAWRKTAC"
     gene            16611..17966
                     /locus_tag="PKN51_07745"
     CDS             16611..17966
                     /locus_tag="PKN51_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNT85029.1"
                     /translation="MAQDGVLTALAAGGLSGMRRLRIDDWSEWWAAVTRPVVLGEFLL
                     MLASGLLAWGVTALLRRHRALPAQAQPGILLGRRGVDGVLFPMLWLTLAWLARLVWLR
                     WEASAPLLRVALPVLLALLVIRVGVKVLQAAFPRARFVRALERSISWLVWGLWVLWLS
                     GLLPVLLEEADHLTWKVGAATLSLRSLLEGGLNVVIALLLALWLSSAIESRLLREVGG
                     GTLSMRKMLANATRAVLVFVAVLVALSAVGIDLTALSVLGGAIGVGIGLGLQRLAANY
                     VSGFVVLAERALRIGDMVRVDGFEGQITDIRARYTVIRSSSGRESVVPNELMVTQRVE
                     NLSLADRRIWQSTTVGVGYGSDARQVQQLLRDAALTVPRVLREPAPSASLSAFGADAL
                     EFTVGYWMSEPEAGVLGLRSEVNLAILDALRRHGIEIPFPQRVLHHPPPAPTPAESRV
                     E"
     gene            18128..>18687
                     /locus_tag="PKN51_07750"
     CDS             18128..>18687
                     /locus_tag="PKN51_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNT85030.1"
                     /translation="MKALVAVKRVVDYNVKVRVKSDGTGVDIANVKMSMNPFDEIAVE
                     EAVRLKEKGVVSEIVAVSCGVAQCQETLRTAMAIGADRAILVETDAELQPLAVAKLLK
                     ALVDKEQPGLVILGKQAIDDDANQTGQMLAALAELPQATFASKVEIADGKASVTREVD
                     GGLETLALTLPAVVTTDLRLNEPRYVT"
BASE COUNT         2517 a         6481 c         7065 g         2624 t
ORIGIN      
        1 agtttggcgg cgaactggac cagccggcgc gcctggtgac cgatggcggc cttgacgtcg
       61 tcgctgaacg gctggccctg ggtgtggctg tagccgtagg ggttgccgcc gaccttgaag
      121 atcaccgggt cggtgaagcc gggcgccacc acgatgccgc cccagtgcat gatggtggcg
      181 tagaaggaca gcagggtgga ttcctgaccg ccgtgggcgt tttgcgccga cgtcatggcc
      241 gacaccgcct tgttggccag accacccttg gcccagacgc cgcccagggt gtcgatgaag
      301 gcgcgcatct ggctggccat gatgccaaag cgcgtcggcg cgacgatcag gtaggcgttg
      361 gcccactcca tgtcggcggc cgtggcctgc ggcagctgcg cggtcttgtc ggcctgcgcc
      421 ttccaggccg gctggccggc caccacggct tcgggggcgg tttcgggcac cttcagcagg
      481 cgcacctcgg cgccggcggc gcgggcggcc tcggcggcga tttcagccat ctggtggttg
      541 gtgccgtagg tggagtagta aacgatggcc agacgaacgc tcatgagggc tcctgtgaaa
      601 cggtttggag gggatcgagg gggtgtcagc ctccagttta ttgggattct ttggggccat
      661 aaatacacaa atccaactca tacaattcca tgattggaac aatgggtgca cccatgaacc
      721 ccatcagcct gcctgccaac gacctgatcc tgtttgccca catcgtggcg gcgggcagct
      781 tcacgcgcgc cgccgaccgc accggcctgc ccaaggccac cctgtcgcgt cgcctgtccg
      841 agctggaaga cgccctgggc gagcgcctgc tgcagcgcag cacgcgccgc ctggcgctga
      901 ccgagttcgg ccagcacatg ctggagcacg cgcagcgtct gctggaggag tcggccgccg
      961 ccacggcgct ggcgcagcac cggcagctga cgccgcaggg cacgctgcgg gcctcgtttc
     1021 cgcccgagtt cagcgcgctg tcgctggtga aggtggtgag cgagttcgcg cgccgccacc
     1081 ccgaggtgcg gctggagctg gacctgtcgg cgcggcgcgt cgatctggtg gccgaacgct
     1141 tcgacctggc ggtgcgcgcc gccacccagt tgcccgacga cagcaccctg gtggcgcgcc
     1201 acatcgtgac gcacgaacac ggcctgttcg ccagcccgga ctacctgcgc cgccatggca
     1261 cgccggccac gccggccgag ctggagcggc acgtcgggct ggccatgctg ggcagcggcg
     1321 gcgagcgcca gccctggcgc ctgacgcgcg gcaaggaacg ctgggaaggc ctgccggccc
     1381 aggtgcactg cgccaactcg gtggggctgc aacaggcgct ggcggtccag ggcctgggca
     1441 tcgtgggtct gtcggcgctg ttcgtgcgcc agagccgcga cgccgactcg ctgcagcgcg
     1501 tgctgcccga ctggtgcctg ccgcccaccg gggtgtggtg cgtgacgccg gggcggcgcc
     1561 tgctgcccaa gcgcaccagc gccttcatcc agatcctgac cgaggtgctg gccgagggcg
     1621 gcgccgcgcc cgccccgctg gccgccggtc accgaccgcg ccgatgagcg ccgggcctgc
     1681 cgggcgtggg ggcggcgccg cgccgccggc accaccggcg gggctggccc tacactcggt
     1741 ggcatgagcg cacccacctc accccaacgt cccgccgtgc tggtggcgcg ggcgatcttt
     1801 cccgacatcg tcgagcagct gtcgcgccac ttcgaggtcg agtccaaccc cgacgacacc
     1861 ctgtggccgc gcgccgagct ggtgcgccgc ctgcagggca agcagggcgt gttcaccacc
     1921 ggcggcgagc gcatcgacgc cgagctgctg gccgcctgcc cggggctgag gatctgcgcc
     1981 aacatggcgg tgggctacaa caacttcgac ctgccggcca tgacggccgc cggcgtgctg
     2041 gccaccaacg cgccgggggt gctgaccgaa accaccgccg acatgggttt tgccctgctg
     2101 atggcggcgg cgcgccgcgt caccgagggc gaacactttc tgcgcgccgg cgcctgggac
     2161 cgctgggcct acgacatgct gatcgggcag gacgtgcacg gcagcacgct gggcatcatc
     2221 ggcatgggcc gcatcggcca ggcgatcgcg cggcgcggcg cgctgggctt cggcatgcgg
     2281 gtgatctacc acaaccgctc gcgcctgccg gcgaccgacg aagccgccct gggcgcgcgc
     2341 tacgtcagca agcaggagtt gctgcaaagc gccgaccacg tggtgctggt gctgccctac
     2401 agcgccgcct cgcaccacac catcggcgct gccgagctgg cgctgatgaa gcccagcgcc
     2461 acgctgacca acatcgcccg cggcggcatc gtcgacgacg ccgcactggc ggcggcgctg
     2521 cgcgaaggcc gcatcgccgc cgccgggctg gacgtgttcg agggcgagcc caaggtgcat
     2581 cccgatctgc tggcctgccg caacgtggtg ctgacaccgc acatcggcag cgccaccgtg
     2641 cccacgcggc gcgccatggc ccagctggcg gccgacaacc tgatcggcta cctggtgcac
     2701 ggcaagccgc tgaccccgct caacccggaa gtcgcggccc tttgatacgg cttcgcgttc
     2761 gaacatgacc cacgaccgct tggtcttcga cgcgcaacga ggtcatgggt gatgggtcgt
     2821 tctgcaagcg agccggcctg aagcttgcga ttgattttgg ccgccggcgc ccatcaggca
     2881 agcgccggca gctatcgata tagtagcaat catggacctc tccaaccctg tctggctgcc
     2941 ctggctggcg ctggccgcac tgttgctcaa cctgctgctg ctgctggcgc tgctgctgcg
     3001 ccgcccgcgc cggccggccg acgtggccgc gcgcgacgaa gtgcggcagt ggctggacca
     3061 gcagggcgag cggctggagc gcgggctgcg ccaggaaatg ggtgagggcg cgcgcagcgg
     3121 ccgccaggag ctggcgcagg cgctggccag cttccagagc gcggtcacgg cgcagggggc
     3181 agaagccgtg cgcacccaga acgcccaggt cgacgcactg gccatgcagc tgacccagct
     3241 gcgcggcacc ctgggcgaca ccctggtcgg gcagttgcag caactggccc tgaccatgac
     3301 ccagcaggcg caggaggcca cgcgcacgca gaacgcgcag atcgacgcct tcgcccagca
     3361 gctggcgcac ctgcgcggca gcctgtcgga gacgctgacg cagcagctgc agcagctcag
     3421 cgaagccaac gcccgccgcg tgcaggagat gcgcgccacg ctcgagcagc agatcggcgc
     3481 gctgcaggcc gccaacagcg ccaagctcga cgagatgcgc cagaccgtcg acgaaaagct
     3541 gcacgccacg ctggagcagc gcctgggcga gcgcttcaag caggtggccg aacggctgga
     3601 gcaggtgcac aagggcctgg gcgagatgca gacgctggcg cagggggtgg gcgacctcaa
     3661 gcacctgctg gccaacgtca agacgcgcgg caccttcggc gaggcgcagc tggggcaact
     3721 gctggagcag gtgttcgcgc ccgagcagta cgcggcgcag gtggccacgc gccccgacac
     3781 gcgccacgcg gtcgacttcg ccatccgcct gcccggccgc ggcgacgacg gcgcgccgct
     3841 gtggctgccg atcgacgcca agttcccgac cgaggactac gagcgcctgc tcgacgccca
     3901 gcagcgcgcc gacgccggcg cggccgaggc ggccggcaag gcgctggaag cgcgcatccg
     3961 gctggaagcg cgcagcatcg ccgacaagta cgtcgagccg ccgcacacca ccgacttcgc
     4021 catcctgttc ctgcccagcg agggcctgta cgccgaggtg ctgcgccgtc ccggcctgat
     4081 ggaggcgttg cagcgcgaac tgcgcgtcac cctggccggg ccgaccacgc tgctggccat
     4141 gctcagcagc ctgcagatgg gctttcgcac cctggcgctg gaaaagcgca gcagcgaggt
     4201 gtggcaggtg ctgggcgcgg tcaagaccga attcgccaag ttcggcgacg tgctggcgcg
     4261 cgtcaagtcg cagacccaga ccgtgctcaa caccctggac gccgcgcaga cgcgcaccaa
     4321 cgtgatgaac cgcgcgctgc gccgcgtcga ggccctgccc gacgagcagg cgctgcagct
     4381 gctgccgggc ggcggcgacg ccgacggcgc cgggggcaat tcttgagcgc tatcacatca
     4441 atagctgccg gcgcagtctg cgcaagcgct gtagccgttt tgaaggctga accgcgatgg
     4501 ccctgagcgg ccgccaggtg ctgcgcctga tgctggcgca ggtgtgcgtg cacagcgcca
     4561 tgaccggcac ccggctggcg gcgccgctct tggcgctcaa gctcggctac agcgcggcgg
     4621 cggtgggcgt gctgctggcg ctgtttgcgc tgtcggcggt gtttctggcg ctgccggccg
     4681 ggcgcctggc cgatcgccac ggcctgcacc tgccgctggc gctggcggtg tcggcggcca
     4741 tgctgggcgc cgggctggcg gcggcctggc cggtgtttgc cgtgctgtgc gcctcggcgc
     4801 tgctggtggg ggcggcggcc aacgcggcgc agatcgcgct gcagcgccac atcggccgcc
     4861 tggccagcga cgccgccgag cgcaagctgg ccttcagctg gatcgccatc gcaccggcgg
     4921 cggccaactt cgccggcccg ctggtggccg ggctgctgat cgaccgcgcc ggaccgcagg
     4981 cgggcgatct gatcggctac cgctgggcct tcgggctgat ggcggcgctg ccgctgctgt
     5041 gctgggcgct gctgcgcggc acgccgcgcg cgccggtggt gccggccgcg gcgggcgagg
     5101 agcgcgcctc ggtgtgggat ttgctgggcc acgccgccat gcgccgtctg ctgctggtca
     5161 actggctgca gtcgatggcc tgggacgtgc acatgttcgt gctgccgctg ctggggcacg
     5221 agcgcgggct gtcggcgtcg gtgatcggcg cgctgcaggg cgcgttcgcg gtcgccgcgg
     5281 cgctggtgcg catggcgctg ccgctgctgg cgcgccggct ggccgaatgg caggtgatct
     5341 acgcctccac cctgctggcg gcctgcgcgc tgctggccta cccgtggatg ccggggccgc
     5401 tgggcatggg cgcctgctcg atggtgctgg gcgtggccct gggcgcggtg cagccgatgg
     5461 tgatgagcct gctgcacgag gtggcgccca tcgagcgcca gggcgaggcc atggcgctgc
     5521 gctcgctgac catgaacgcc tccagcttca ccatgccgat gctgttcggc tccatcggcg
     5581 ccgtcatcgg caccggcggg ctgttctggc tggtggcgct ggtgctggcg ctgggcgcgc
     5641 gcagcgtgcc ggccctgggc ggggcggcgc ggccaccgtc ggcctgagac cgaaccaccc
     5701 gcccggcccg gcccacgccg gcttcgggca cggcggcgcc gccgctgccg gaaccgatca
     5761 gtcgacttcg gcgatcagct cgatctcgac gcaggcgccg agcgggatct gcgccacgcc
     5821 gaaggcgctg cgcgcgtgct tgccggcgtc accgaacacc tggcccagca gctcggagca
     5881 gccgttggtg accaggtgct gctcggtgaa gtcgggcgtg ctgttgacca ggctcatcac
     5941 cttgacgatg cggcgcacct tgcccagatc gcccaccgcc gcctgcagcg tgcccatcag
     6001 gtcgatggcg actgcgcgcg cggcctgcgc cccttcggcg gtggtcatgc tggcgcccag
     6061 cttgccgacc cagggctggc cgtcccggcg cgcgatgtgg ccggacagga acaccagtgg
     6121 gccgctgcgc acgaagggca cgtaggcggc ggcgggggtg ctgacgggcg gcaggctgat
     6181 gcccagggct ttgagctgct cggcgggatg ggtcatggtc ggggtctccg gaagaagggg
     6241 cggatcaccg agcggtggca acggctcgga ccgcggggcg gccattgtcg caccggctgg
     6301 cgtgggcgcg gcgccacgcc ggcgcggcgt ttgcggagat actggcaccc aggcgctggc
     6361 ccggcgcgct cgcgggctta ggctcccaag cggccctggc gcttatctgg tctgcgccag
     6421 aagctatcaa agccatagcg tccgatgcgg ttttccgaac ccccgccaaa ccctgccgcg
     6481 ctcgggcgct ggcgcggcca tgcgctgacg gcgctgctgg cgggcggcgt gctgggcgtg
     6541 gcggcgcagc tgcagcaggc gcgcctgtgg ccggcctggg cctatgcgct gacgggcctg
     6601 ctggcgctgc tgctggcggt ggtggcctgg cgcccgcgcc gggccggggc gcgcgcgctc
     6661 gggctgtggc tgctggccgg ggcgctggcc gccggcgcca gcaccggctg gcgcgccggg
     6721 ctgcgccagg ccgagcggct ggcgccggcg ctggaagggc gcgacctgcg gctgaccggc
     6781 cgcatcgccg gcctgccgca gcgcggcgaa gccggctggc gctttcgctt cgacgtcgag
     6841 cgggccagcc tggacggcgc gccggtgcgc gtgccgccgc gcgtgcagct gggctggtac
     6901 ggctgggtgg cgacggtcga cgaaaccgac gccgagcgcc ccgtggccgc ggcgctgcgc
     6961 agtggcgacc gctggcagct gacggcgcgg ctgaaggcgc cgcacggcaa cctgaacccg
     7021 cacggcttcg acctggagct gtggctgtgg gagcagggcg tgggcgccag cggctacgtg
     7081 cgcagcggtc cgcgcgaggc gccggcgcgc cggctggagg aaggcggcgc ctcgctgccg
     7141 cgctggcggc aggcggtgcg cgaccgcatc ctggacggcg ccgacgccgg cgacgccacg
     7201 gcgcgccggc tggccggcat cgtggcggcg ctggtgacgg gcgatcagaa cgccatcgag
     7261 cgcgccgact gggacgtgtt tcgcgccacc ggcgtggcgc acctgatgag catctccggc
     7321 ctgcacatca ccatgttcgc ctggctggcg gcggcgctgg tgggtgcgtt gtggcggcgc
     7381 tcggcgcgct ggggctggcc ggcgcgcgcc aacccctgcc tgtggctgcc ggcgccgacg
     7441 gccgcgcgcg tcggcggcgt gctgctggcg gccggctacg cgctgttcag tggttggggc
     7501 gtgccggcgc agcgcacggt gctgatgctg gccacgctga cgctgctgca cgtcgccggc
     7561 ctgcgctggc cctggtggct gaatgcgctg gcggcggcgg tggtggtgct ggcgctggac
     7621 ccgtgggcgc tgatgcagcc cggcttctgg ctcagcttcg tcgccgtggc ggtcctgttt
     7681 gctaccgatt cgagagcggc cggcgcacaa cagacgggcg ccagagcccg atggcttgcc
     7741 ttgctgcggg agcagggcgt ggtcacgctg gcgctcacgc cgctgtcgct gatcctgttc
     7801 ggtcaggctt cggtggtggg cctgctggcc aatctgctgg ccattccctg ggtcacgctg
     7861 ctggtcacgc cgctggcgct gctgggcgtg ctgtgggcgc cgctgtgggc actggccggc
     7921 gccgcgctgt ggccgctggc ggcgctgctg ggctggctgg cggcctggcc ctgggccagc
     7981 gtgtcgctgc cggcggcgcc gtggtggctg ggcgtggcgg cggtggccgg cgggctgctg
     8041 ctggcgctgc gctggccgcc ggcgtggcgg ctggccggcc tgccgctggt gctgccgctg
     8101 gcgctgtggc agccggcgcg gccggcgccg gggcagttcg aattgctggc ggccgacgtc
     8161 gggcagggca gcgcggtcct cgtgcgcacc gcgcgccaca gcctgctgta cgacgccggg
     8221 ccgcgctact cgctcgaaag cgatgccggc catcgcgtgc tggtgccgct gctgcgcgcg
     8281 ctgggcgagc gcctggacag cgtggtgctg agccaccgcg acagcgacca caccggcggc
     8341 gctcgcgccg tgttggcggc gcagccgcag gccgagctgc gcgcgtcgat cgaggacggg
     8401 cacgagctgc aggcgctgcg cccggccacc cgctgccagg ccggccagcg ctggcagtgg
     8461 gacggcgtgg ccttcgagct gctgcacccc gacgccgccg accacgcccg cccgctcaag
     8521 tccaacgccc tcagctgcgt gctgcgcgtg cgtgcggtcg acggcgccgc cgccttgctg
     8581 accggcgaca tcgagcaggc gcaggagcag gcgctggtgg cgcgcacgcc cgagctgcgc
     8641 gccgacgtgc tgctggcgcc gcaccacggc agccgcggtt cgtcgtcgac cgcgctgctg
     8701 gcggcagtgc gcccgcgctg ggtgctggtg caggccggct accgcaaccg cttcggtcac
     8761 ccggcagccg aggcgctggc gcgctacgcc gccgtcggtg cgcagccggt cggctcgccg
     8821 cgctgcggcg ccatgcgctg gcacagcgcg cagcccggcg cgctgcgctg cgaacgcgac
     8881 acggcggcac gctactggca tcatcaaccg ccatgaagac gctactgatc gtctaccaca
     8941 gcctgaccgg cggcacgcgc cagatggccg aggcggcggc ccaggccgcg cgccaggagg
     9001 gcggcgtggc ggtgcgcctg ctgaccgccg cgcaagccgg cccgccggac ctgctggcag
     9061 ccgacggcta cctgttcgcc acgccggaga acctggcggc catggccggg ctgatgaagg
     9121 acttcttcga ccgctgctac tacccggtgc tcgaacgcat ccaggggcgg ccctacgccg
     9181 ccatgatctg cgccggcagc gacggcgccg gcgcgctgcg ccagctcgaa cgcatcgcca
     9241 ccggctggcg gctgaagcaa gtggcgcccg gcctgatcgt ctgcacccac gcccagacgc
     9301 ccgagcgcat cctggcgccc aagaccatcg agccgccgga cctggcgcgc tgcgccgagc
     9361 tgggcgccgg gctggcggcg gggctggggg cgggggtgtt ctgagcgcgg ccgcgccggc
     9421 ccccgctacg cgcccagcgc cgccgccact tcctgcccca gctcgatcac ggccatggcg
     9481 tagtagctgc tccagttgta gcgggtgatg acgtagaagt tttcggtgcc ggccacgtag
     9541 ctgggttcgg cggcgccgtt ctgcagctcg atcagcgcca gcgggccgtc gtgggcctgg
     9601 ccggcggcgt ccaggcgggc gcccagctcg gcaaagcgcg ccggccggaa cgagggcagg
     9661 atgtcgggcg ccagcaggcg gtccaggtcg gcctcgggcg ccagttgcac cgggtaatgc
     9721 gtgggctggc cgggctgcca gccgtagccc tggaagtagt gcgccaccga gccgatggcg
     9781 tcgccttcgc tgccccacag gtcgatgtgg ccgtcggcgt cgaagtcgat ggcgtagcgc
     9841 acccagctgg agggcatgaa ctgcggcatg cccatggcgc cggcgtagct gcccagcggc
     9901 tgctgcgggt ccatgccgct gcgctgacac agcgacaggt actgcgccag ctcgtcctga
     9961 aagaacgcct gacgcgccgc cgcccgcggg tgctggggcg ggaagtcgaa gctgagcgtg
    10021 gccagcgcgt cgagcacgcg aaagctgccc atgttctggc cgtagatggt ttccacgccg
    10081 atgatgccga ccacgatctc gggcggcacg ccgtagtcct gctgggcgcg cgccagcgtc
    10141 gcgcggttgc gctgccagaa gcgggcgccg gcgcggatgc gcgtggcatc gatgaagcgg
    10201 ctgcggtagg cgcgccagtt cttggccgtg ccgcgcgccg gcggcagcat caggcgcggc
    10261 acgttgggca ggtgctgggc ctggccgatg gtgtcgcgca cccagtcggg cggcaggtcg
    10321 cgccgggccg ccatgtcttc ggcccagcgc agggcgtcgg ggcggctggc gtagctggac
    10381 ggggcggcct cgggcaggcg acggcgtttg gggccgttct gggctgcagc gctgacgggg
    10441 agtgcgccag cagctatgca aatggtagca agcaggcgac gtcgatcaaa gggcaggcgg
    10501 ggtgcggtca tggcgggtgg ggtggcgcgg ccagggcagg cggcgcagtt cgcgttgcag
    10561 tgtagtcagc cggggcgccg ccgcctgcga cggggcgtaa cgcagggttt ccagggccag
    10621 cagccagtcg gtgagcggct ggcgcagggc cgcgggcagc gtgctgtgcg ccacctgctg
    10681 cgccagcgcg cgtggcggcg cctgcggcgg cgcattcagg ccggcctggg ccagcagccg
    10741 gcgcgcgcgc gccagcaggc gcagccaggg gtcgtgctgg ctgcgttccc agcgcgccca
    10801 ggccatgccg agcagggcgg cggccaccag caggccagcc agcaccttgc ccaggtcggt
    10861 ccagttgggg ctggcaaagc ccagctggcg cagcaggtcg agctgccggc tctgggtgta
    10921 gttgagcacc cattggttcc agccgttgtt ggccgcttcc cacaggctgc gcagctgcgc
    10981 catcagggtc gggctgaggg tgccgagggc gccggccacc aggccttccg ggcggcgcag
    11041 ccgctgcagc tggccgatgc gcgccggctg cacggcggcc gtggggtcca cgcgcagcca
    11101 gccgcgcccg gggtcccaga cttcggtcca ggcgtgggcg tcggcgttgc gcaccaccca
    11161 gtagccgtcg acaccgttga gctcgccgcc ctgaaagccg gtgacgatgc gcgccggcac
    11221 cccggcggcg cgcagcagca ccacgaaggc cgagctgatg tgctcgcaaa aacccgcctt
    11281 ggtgtcgaac cagaactcgt cggcgctgtg ctggccgctc acgccgggct ccagcgtgta
    11341 gtggtagccg ccgctgcgca ggcgctccag cgccgcctgc accagggcca gggtgttgcc
    11401 ggcgccgtgc cggtcgcgca gctgctgggc cagcgccagc gtgcgcgggt tgtggtcggc
    11461 cggcaggcgc agctgctcgt ccagatcgcg ccgcgcctgg ccgtcgctgc cgaccaggcc
    11521 gtagtggaac agcgggtagc tttcggcgcg gtagcgcagc agctcgctca ccgggcgcgg
    11581 gctgagccat tgcagatcac cggtcaggcg cgcgctgcgg ccttcgggca gctcgggcgc
    11641 gcgcggcgcg acgtcgagcg tgagcagcca ggggcgaagg ctgggttcga gcgtgacctc
    11701 atagcgcacc ggttcgccct gcacgcgcag atcggacggc ggcggcaggc ccaggccttc
    11761 gaagccgggg ccgccgcgcg ccgtccactg ccggccgtcg aagcggctca gcaccggccc
    11821 gcggaagtac agctggccct gcggcggcgg cgggccgtcg aacttcacgc gcagggcaat
    11881 gccgtcgtcc agcgccagct tggccacctg gccgacctcc atggtcgagg acaggccgct
    11941 gcgaccggtc agggcgtcgc tgggcatgcc ccacagcggc gccaggcgcg gaaagaacag
    12001 gaacagcgcc agcatcaccg gcgtgccggc cagcgccatg cgcagcgcca ccgcggccga
    12061 ttcgcgcagg ctgggctcgc ccaccggacg gtgcgcgtgc accagcgccg tcagcaggcc
    12121 cagcaggccg atcagcatgg cggtggccac cggcagcgtc tgcgcgtaga agaagttggt
    12181 cagcagcgtg aagaagccga ggaagaacac caccagcgcg tcgcgccggg cgcgcagctc
    12241 cagcgtcttg agcgccagca gcaccaccac cagcgtcacc ccggcgtcgc gccccagcag
    12301 cgtgcggtgc gaagtccagg tgccgaccag ggtcagcgcc agcagcagca ccagccaggc
    12361 gcgccgcggc agcggccggc tgcgcagcgc cagcagaccg cgccaggcca gcacggcggc
    12421 cgccagcgcg ctgcaccaca gcggcagatg cggcaccagc ggcaggatca gccagccgat
    12481 cacggccagc agaaacagcg tgtcgcgcgc ttcgcgtggc aggcggcgga gggcggtgag
    12541 cgtcatcggg cggcggcgtg gggcggtggg ggtcaggcgc tggccagcgc ctgcagacag
    12601 cgcagccgct gggcggcacc gccggccggg gcgatttcgc gcccgggcag gcgcaggccg
    12661 tagtcgagct gctgcgcgtc ggccgccagc acccaggcgg tcaggcgcgc caggcgcgct
    12721 tcggggtcga gcgcgccggc gcgcgcgtag tccagccaca gcgtctggcg ctgcaggtgc
    12781 agggtgtcgc gcgacaccgg cgcttcggcg cgggcgaact tcttccacac gatcaggcgc
    12841 gccgggtcgc cgcgctggta ggcgcgcacg ccgtcgaatt cgccactggc gcgcgcgccg
    12901 gccgccacgc cgctgccggc gcgcggctcg cccggcggca gcggcggggc ctgcggctcg
    12961 ggcgccggat acaccagcac ctgggcggcc gggcgccaca ccgtccagac gcggaagttg
    13021 ccgagcggaa agcgggtctc gaccgtcagc gccggcaccc ggtgcaggcc gcgccgcgcc
    13081 ggctgaaagc tgacctgcac cgtggcctgc ccctgcgccg gcacgtcggc ccaggaccag
    13141 tgcgcgcctt ccagcagggc cacgccgatg gcgtggcggg gcgtgcggcg ggtgctggtc
    13201 agcttgacgt ccagcaccgc cagcgcaccg gcgaacagtg gctgcggcgg cagcagttgc
    13261 aggtgcaggc cgcgcagcgt gtggtggccg accaccatgc cggcggcggc gctgccggcc
    13321 agcagaaagg tcagcaggta gcccaggttg agctggtagt tgatggcggc gatcagcagc
    13381 acgagcagcg tggcggccag catccagccg gcgcgcgtgg gcaggatgta gacgttgcgc
    13441 tgcgtcagca gcagcgtgtc ggtcagcggc aggcgcgact gccaccaggc ccgcaggcgt
    13501 gcgcgccagg cccaccaggg gactgcgggt gcggattcag aagccgggct ggagcgcacg
    13561 tgtcgagcgg tgggcgtgag ggttcgggtc gtcaatccag cgtccgggcg cagaggtttc
    13621 ggaaggggcg ctgtacatgg atgtggatga aagggtctgg cgcaccatgg gcccatggcc
    13681 cgtcttgggt agcccgcaag cgccgtgggt gtggtctgtc cggcgttcct tgcgaatccc
    13741 tggcgctttg ctgcgcatgg aggtcgggcg ccgttcaggc cagtggcacg gcgtcgatca
    13801 tggcgcgcac ctgctccagc gcgccgcggc cggactggct gaccggcacc atgcggtgcg
    13861 ccacgcattg cggcaggatg gcctgcacat cgtccggggc cacgtagtcg cgcccggcga
    13921 tcagggcgcg tgcgcgggcc gcgcgcacca gggcgatgcc ggcgcgcggc gacaggccct
    13981 gcatgaacca gcggccgctg cgggtggcgg cgatcaggtc ctgcacgtaa tccagcagcg
    14041 gctcggccac gtgcacgcgc gccaccgcct gttgcagcgc ggccagcgcg ctggcatcga
    14101 gcagcgcggg cagccggtcg agcagctcgc gccggtcgtg gccggccagc agggtgcgct
    14161 cggcggcgcg ctcggggtag cccaggctga tgcgcatcag aaagcggtcg agctgcgatt
    14221 ccggcagggc gtaggtgccc agctggtcga acgggttctg cgtggcgatg aggaagaacg
    14281 gccagggcag ggggcgggtt tcgccctcca cgctgacctg ctgttcctcc atcgcctcca
    14341 gcagggccga ctgcgtcttg gggctggcgc ggttgatctc gtcggccagc agcacctggg
    14401 cgaacaccgg gccggggtga aacacgaagg cctccttgcc gcggtcgtac accgacacgc
    14461 ccgacaggtc gctgggcatc aggtcggcgg tgaactgcac gcgcgagaac tgcaggccga
    14521 aggtgcgcgc cagcgcgtgc gccagggtgg tcttgccgac gccgggcacg tcttcgatca
    14581 acaggtggcc gccggccagc aggcaggtca cgccgtcctg cacctgggtc ggtttgccca
    14641 ccaccacggt gttaagctgc gccagcacgg cttggatttt ggatgtcacg tccatgcaag
    14701 gaaggtaacg gatattcggt gccgtattct gtgcgcgtgg agactgccat gagtagaacc
    14761 ggatatttca cacaccccga ctgccgcaag cacgacatgg gcgccggcca tcccgagtgc
    14821 ccgcagcggc tcgacgcgat cgaggaccgc ctgctgatca ccggcgtggc cgacgccctg
    14881 gagcgccgcg aggccacgcc ggccgccatg ggcgatctgc tgctggcgca tggccgccgc
    14941 cacgtggcgg ccctgcgcgg cctgaacgac atcctgctgg aggagatgga cgccggcggc
    15001 cccgaccgct attcgctcga cccggacact tcgatgaacc gctacaccta ccgcgccgcg
    15061 ctgctgtcgg ccggcgctgc catcgacgcg gtggacgcgg tggtggcggg cgagctgacc
    15121 aacgccttct gcgccacccg cccgcccggc caccacgcca cgcgcaacca ggccatgggt
    15181 ttctgcttct tcagcaacgt ggcggtggcg gccaaatacg cctgcgagcg ccacggcctc
    15241 aagcgcgtgg ccatcgtgga cttcgacgtg caccacggca acggcaccga agacatcgtg
    15301 gccggcgacg aacgcatcct gatggtgagc ttcttccagc acccgttcta ccccgaaggc
    15361 ggcgcgcaga agcaggacgc caacctggtc aactgcccgg tgccggccta cacacgcggc
    15421 atggacgtgc gcgaactggt cgagctgatg tggatgccgc ggctggagca gttccagccc
    15481 gagctggtgt tcatcagcgc cggcttcgac gcccaccgcg aggacgacat gggccagctc
    15541 ggcctgaccg aatccgactt cgcctggatc accgagcgcg tcaagggcgt ggccgagcgc
    15601 tttgcccagg gccgcatcgt gagctgcctg gaagggggct acaacctcaa cgcgctggcg
    15661 cgcagcgtgg aggcgcacct gcgcgtgctg gccgaggtgt gatccggaga tgtgaataga
    15721 atcggcttcc agcggccgat cggcaagcgc cagcagctat cgtatttgta gtgcatgacg
    15781 gcgccgccgg gtcgccgccg gccaccggac ggagccatca ccatgagcgc atctccagcc
    15841 atacctgccg agcccctggt gcgccatcag cgcgatgcgc gcggcgtgca ccggctgacg
    15901 ctgaaccagc ccaagtcctt caacgtgctg agcgaggcca tgctggccga gctgcagcgg
    15961 ctgctcgacc agatcggcgc cgacccggcc gcgcgcgcgg tggtgatcgg tgccgaaggc
    16021 aaggccttct gtgccggcca tgacctcaag cagatgaagg ccagtccgcg gctggactat
    16081 taccaggcgc tgtttgccca gtgcagccgc atgatgctgt cgatcctcag gctgccggtg
    16141 ccggtgctgg cacgcgtgca gggcctggcc acggcggccg gctgtcagct ggtggcacag
    16201 tgcgatctgg cggtggcggc gcagggggcg cgcttcggtg tcaacggcat cgacgtcggc
    16261 ctgttctgca gcacgcccag cgtgccgctg gtgcgcaaca tcgcgcccaa gcaggccatg
    16321 gagatgctgc tgactggcgg cttcatcggc gccgaggagg cgcaggcgcg tggcctggtc
    16381 aaccgcgtgg tgccgcccga acagctcgac gagacggtgg aggcgctggt cggcgccatc
    16441 ctggccaagc cgcgtgcggc catcgccgcc ggcaaggagc tgttctatcg ccagcgcgag
    16501 ctgggcatcg aggccgctta ccagctggcc ggccagacca tggcctgcaa catgatgctg
    16561 gacgtcaccc aggaaggcgt gcaggccttc atcgacaagc gggaaccggc gtggcgcaag
    16621 acggcgtgct gaccgcgctg gcggccggtg ggctgagcgg catgcgccgg ctgcgcatcg
    16681 acgactggag cgaatggtgg gcggccgtca cgcggccggt ggtgctgggc gagttcctgc
    16741 tgatgctggc cagcggcctg ctggcctggg gcgtcacggc gctgctgcgg cgccaccgcg
    16801 cgctgccggc gcaggcccaa ccgggcattc tgttggggcg ccgtggcgtg gacggcgtgc
    16861 tgtttccgat gctgtggctg accctggcct ggctggcgcg cctggtctgg ctgcgctggg
    16921 aagcttcggc accgctgctg cgcgtggcgc tgccggtgct gctggcgctg ctggtgatcc
    16981 gggtcggggt caaggtgctg caggcggcgt ttccgcgcgc gcgcttcgtg cgcgcgctgg
    17041 agcgcagcat ttcctggctg gtctggggcc tgtgggtgct gtggctgagc ggtttgctgc
    17101 cggtgctgct ggaagaggcc gaccacctga cctggaaggt cggcgcggcc acgctcagcc
    17161 tgcgcagcct gctggaaggg gggctgaacg tcgtcatcgc cctgctgctg gcgctttggc
    17221 tgtcgtcggc catcgagtcg cgcctgctgc gcgaggtcgg cggcggcacc ctgtcgatgc
    17281 gcaagatgct cgccaacgcc acgcgcgcgg tgctggtgtt cgtggcggtg ctggtggcgc
    17341 tgtcggcggt gggcatcgat ctgaccgcgc tgtcggtgct gggcggagcc atcggcgtcg
    17401 gcatcggcct gggcttgcag cggctggcgg ccaactacgt gtcgggcttc gtggtgctgg
    17461 ccgagcgcgc actgcgcatc ggcgacatgg tgcgcgtcga cggtttcgaa gggcagatca
    17521 ccgacatccg cgcgcgctac accgtgattc gcagcagttc ggggcgcgag tcggtggtgc
    17581 ccaacgaact gatggtgacg cagcgggtgg aaaacctgtc gctggccgac cggcgcatct
    17641 ggcagtcgac caccgtcggt gtcggttacg gcagcgatgc gcgccaggtg cagcagttgc
    17701 tgcgcgatgc ggcgctgacg gtgccccgcg tgctgcgcga gccggcgccg tcggcctcgc
    17761 tgtcggcctt cggtgccgat gcgctggagt tcacggtcgg ctactggatg agcgagcccg
    17821 aagccggggt gctgggcctg cgctccgagg tcaacctggc gatcctcgac gcgctgcgcc
    17881 gccacggcat cgagatcccg ttcccgcagc gcgtgctgca ccatccgccg cctgcgccga
    17941 cgcccgctga aagcagggta gaatgaaaaa gcacgatcgt tcgattttgg tggatctgtc
    18001 gcttgagtga tgtccgtcag gcggcccgcc acaggtcgtg aactagaatg atttcgagtc
    18061 gccacgcagc gggaaactgc cgcgccggca aacgtcaagt gaacagcagc ccaggagatc
    18121 tgtacccatg aaagcactcg tcgccgtcaa gcgcgtggtg gactacaacg tgaaggtgcg
    18181 cgtcaagagc gacggcaccg gcgtggacat cgccaacgtc aagatgagca tgaacccgtt
    18241 cgacgaaatc gcggtggaag aagccgtgcg cctgaaggaa aagggcgtgg tcagcgagat
    18301 cgtcgccgtc tcctgcggcg tcgcccagtg ccaggaaacc ctgcgcaccg ccatggccat
    18361 cggcgccgac cgcgccatcc tggtcgaaac cgatgccgag ctgcagccgc tggccgtggc
    18421 caagctgctc aaggctttgg tggacaagga gcagcccggc ctggtgatcc tgggcaagca
    18481 ggcgatcgac gacgacgcca accagaccgg ccagatgctg gccgcgctgg ccgagctgcc
    18541 gcaggccacc tttgccagca aggtcgagat tgcggacggc aaggccagcg tcacgcgcga
    18601 ggtcgacggc ggcctggaaa ccctggccct cacgctgccg gcggtggtca ccaccgacct
    18661 gcgcctgaac gagccgcgct acgtcac
//
LOCUS       DAOQTZ010000052        18678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3431_length_18678_cov_13.049992, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000052 DAOQTZ010000000
VERSION     DAOQTZ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 18678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18678
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3431_length_18678_cov_13.049992"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1844
                     /locus_tag="PKN51_07755"
     CDS             3..1844
                     /locus_tag="PKN51_07755"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNT85031.1"
                     /translation="MDNAADEALAGYGKKIRVTLHADASVSVEDDGRGIPYGLHPEEK
                     APVLELVFTRLHAGGKFDKGAGGAYSFSGGLHGVGVSVTNALGKRMEVTSHREGMVAT
                     LAFEGGDVVQKLHLRKQAEGDRKQGTTVRVWPDPKYFESPQLPMAELVHLLRSKAVLM
                     PGVTVQLIDEKKKDTQSWQYKGGLRDYLEQTLPADAVIPVFEGEGFAGKDDGSFAEGE
                     GAQWAVAFTEDGAPVRESYVNLIPTSAGGTHDSGLRDGLFQAVKGFIELHALLPKGVK
                     LMPEDVFARASYVLSAKVLDPQFQGQIKERLNSRDAVRLVSGFVKPALELWLNQHVEW
                     GKRLAELAIKAAQTRQRAGQKVEKRKGSGVAVLPGKLTDCESRDLTVNEVFLVEGDSA
                     GGSAKMGRDKETQAILPLRGKVLNTWEVERDRLFANTEIHDISVAIGVDPHGPQDEVD
                     LSNLRYGKVCILSDADVDGAHIQVLLLTLFFRHFPKLIEAGHVYVARPPLYRVDAPAR
                     GKKPAAKLYALDEGELAAILDKLRKDGVAEGKWTISRFKGLGEMNAEQLWDTTLNPDT
                     RRLMPVQLGAFDFAATAAEITKLMGKGEAAARRELMELHGDAVEVDV"
     gene            1893..2222
                     /locus_tag="PKN51_07760"
     CDS             1893..2222
                     /locus_tag="PKN51_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNT85032.1"
                     /translation="MTAFDWTNITGFDWDAGNARKNDKHGVSMAEAEQVFFNQPLLVV
                     SDDKHSQKEARLHALGRTDGGRLLHLTFTLRQQGSLIRVISARAMHRKERTIYEQADQ
                     EGSAPRQ"
     gene            2182..2445
                     /locus_tag="PKN51_07765"
     CDS             2182..2445
                     /locus_tag="PKN51_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNT85033.1"
                     /translation="MSKPTKKVPHLGSEAQERAFWETHDSTDYVDWSKAQRVVLPNLK
                     PTTKTISLRLPQHLLDSIKAAANARDVPYQSLIKVWLQDKLRG"
     gene            2549..2761
                     /locus_tag="PKN51_07770"
     CDS             2549..2761
                     /locus_tag="PKN51_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNT85034.1"
                     /translation="MRTTVTIEDTLYDQALELADPDMDKAELFREAIRTFIRVQSGKR
                     LARLGGQAPQMAEVPRRRAPARAPAA"
     gene            2758..3150
                     /locus_tag="PKN51_07775"
     CDS             2758..3150
                     /locus_tag="PKN51_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HNT85035.1"
                     /translation="MTPAPEVLVDTSVWIEHFRRGSAPLAELLAGDAVRMHPLVLLEL
                     ACGSPPAPRARTLADLARLRPAPQAGQAELLAFIEQEQLFDSGCGAVDIHLLASARLL
                     PGARLWTLDKPLHALARRLGVAWVVPTR"
     gene            3225..5615
                     /gene="parC"
                     /locus_tag="PKN51_07780"
     CDS             3225..5615
                     /gene="parC"
                     /locus_tag="PKN51_07780"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNT85036.1"
                     /translation="MSDQTVLDLSPADPGDDVALATYAQRAYLEYALSVVKGRALPDV
                     CDGQKPVQRRILYAMERMGLGYGGPAGNTAAKPVKSARVVGDVLGRFHPHGDSAAYEA
                     LVRMAQDFSQRYPLIDGQGNFGSRDGDGAAAMRYTEARLARITRLLLSEIDQGTVDWV
                     PNYDGSTEEPRQLPARLPFALLNGASGIAVGLATEIPSHNLREVADACIALVKNPKLA
                     DDELFALLPGPDYPGGGQIISSDSEIRAAYQSGRGSLKVRARWVIEDLARGQWQLVVT
                     ELPPGTSAQKVLEEIEELTNPKVKTGKKAVTQEQNQLKGTILAVLDGVRDESSKDAPV
                     RLVFEPKSRTIGQDELANTLLAHTSLECSSPINLTSVGLDGRPVQKSLRQMLAEWIEF
                     RQSTITRRSQHRLQKVLERIHILEGRQLVLLNIDEVIAIIRTADEPKAALIARFALTE
                     RQADDILDIRLRQLARLEAIKIEQELSELRKEQGSLEDVLASPATLRRLMIKEIEADA
                     KEFADPRRTLIQAEKKAVVEVKVQDEPVTVVVSEKGWVRARSGHGHDAAGFAFKAGDG
                     LYGTFECRTVDQLLVFGSNGRVYSVAVASLPGARGDGQPITTLIELEAGTQPLHYFAG
                     PAGAALLLSGSGGYGFVARVEHMLSRNKSGKAFISIGAGEQVCRPSLVALDPEQKTAP
                     TQTGQAPAAIGSIAATHVACASVGGRILTFDIGELKLMEKGGRGLTLIDLEPKDSLAG
                     AAAYTRSVRLLGVGRGGKPREEQLEIRSLNNARAARGRKGRAADLGFKPHTIVRVE"
     gene            5615..5809
                     /locus_tag="PKN51_07785"
     CDS             5615..5809
                     /locus_tag="PKN51_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85037.1"
                     /translation="MGGLDLSPLGIVLLVVTGVLSFALGRWWSRGWRARRQARRAAEA
                     RKLETRQQRRAREREQAKRR"
     gene            complement(6299..7117)
                     /locus_tag="PKN51_07790"
     CDS             complement(6299..7117)
                     /locus_tag="PKN51_07790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT85038.1"
                     /translation="MSKTLLAAAAAAVLTGALSLPAQAQNVAIVNGKPVPKARLDALE
                     AQIKAQAARTGQPIPPEVQGQLREEIIAREVFMQEAQRRGLEASPDYRAKMDLARQSV
                     LIGELFADFQKNNPVSDAEAKAEYDKVIGSQVPAAGAKEIKARHILVEKEDEAKAIIA
                     QLKKGAKFEELAKKQSKDPGSGAQGGDLGWANPSGYVPEFADALGKLAKGQLTDAPVK
                     SQFGWHVIRVDDLREAKAPELPSFEAVKPQIVQQLQQQKLVQFQQDLRTKAKVE"
     gene            complement(7172..7438)
                     /locus_tag="PKN51_07795"
     CDS             complement(7172..7438)
                     /locus_tag="PKN51_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNT85039.1"
                     /translation="MSTELADRMAARLRERLQPSALEVLDESWQHEGHAGANGTGFGT
                     HFRVRIASPLFDGKSRVVRHQLVYQALQDFIERDGVHALAIEAR"
     gene            complement(7438..7995)
                     /locus_tag="PKN51_07800"
     CDS             complement(7438..7995)
                     /locus_tag="PKN51_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HNT85040.1"
                     /translation="MKALLDFLPILLFFGAYKLYGIYPATAVLMAATCVQMGIIYAID
                     RKLTAMHKATLALILVFGTLTLVLQDERFIKWKPTVLYAAMALALAVALWAYRKNFLK
                     IMLGSQLSLPEPVWHRLTLAWVLYCLFMATLNGYVAAFWSTDAWADFKLWGYVFPVAF
                     IVGQGIYVARHLSDEPAAGNPPEST"
     gene            complement(8009..8419)
                     /gene="msrB"
                     /locus_tag="PKN51_07805"
     CDS             complement(8009..8419)
                     /gene="msrB"
                     /locus_tag="PKN51_07805"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNT85041.1"
                     /translation="MSHPVQKSDAEWRALLADKGAEPLAFQVTRHAATERPFSGRYER
                     HWQDGSYHCICCGARLFESDTKFDAGCGWPSFWQAALPGAIEERRDTSHGMVRVETLC
                     ARCGAHLGHVFPDGPAPTGLRYCMNSAALDFEPR"
     gene            complement(8416..9900)
                     /locus_tag="PKN51_07810"
     CDS             complement(8416..9900)
                     /locus_tag="PKN51_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNT85042.1"
                     /translation="MLNRTDSVQAVDLGLTWDNAFERLGEGFFTRLAPLPLRGEAYWV
                     ARSAAMARELGLADDWWQVQGALPAVSGGQPLAGSAPLASVYGGHQFGAWAGQLGDGR
                     AILLGEVRTTGGGSQELQLKGSGLTPYSRMGDGRAVLRSSIREFLCSEAMHALGVPTT
                     RALCVAGSDEPVRRERLETAAVVLRAAPSFIRFGHFEHFCHSGRHEALRTLADFVIAR
                     FYPDCRDASNPYAALLGAVTQRTAALLAQWQALGFMHGVMNTDNMSILGLTIDYGPFQ
                     FMDGFDPARICNHSDHLGRYAYQRQPEIAHWNLFALGQALLPLIRQPDAALAVLQPFP
                     ALFSAELDRRMALKLGLPQASDGSRQLVQALLELLTRERTDWTIFWRRLAQQVAGDLR
                     QPVTDLFHDRAAIDRLLLQYQELLAQSNRAQTADLMLKTNPGLVLRNHLAEQAIALAR
                     QHDFSMVQSLHDALQRPFDEQPEHADWCNFPPDWASQIQISCSS"
     gene            10001..11638
                     /locus_tag="PKN51_07815"
     CDS             10001..11638
                     /locus_tag="PKN51_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HNT85043.1"
                     /translation="MLGQMQNHPLLTSMLLEFAERHHGDGEIVSRRVEGDIHRYTYRD
                     LARRSRQLAATLDKAGLQQGDRVATLAWNGYRHLELYFGVTGSGRVVHTLNPRLHPEQ
                     LVWIINHAEDAVLCFDMTFLPLIQAVHAKCPSVKTWVALCEADKLPADSGIPQLVSYE
                     AWIGTSTDQYVWPKFDENSAAGLCYTSGTTGNPKGALYSHRSTVLHAYGGALPDALNL
                     SARDSMMPVVPMFHVNAWGLPYVAALTGCKVVFPGPAMDGKSIYELMEAEGVSMAAGV
                     PTIWQMLLGHVQGNKLKFSTLNRTVIGGSACPPAMIDTFREEFGVDVLHAWGMTETSP
                     LGSVSTLKNKHLKQPREQQLAIRVKQGRAICGVDMRIVGDDGNEQPWDGKTFGDLQVR
                     GPWIIESYFKGETPLVTDEHGHRWFPTGDVATIDADGYMQITDRSKDVIKSGGEWISS
                     IDIENIAMAHPAIAMAACIGMPHPKWDERPIVAVVRKPGAEVTREELLAFYEGKTAKW
                     QIPDDVVFVDAIPLGATGKMLKTRLRELLKDYKLPTA"
     gene            11752..12990
                     /locus_tag="PKN51_07820"
     CDS             11752..12990
                     /locus_tag="PKN51_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT85044.1"
                     /translation="MKFVWKSVMTASALLLGGAALAQQGEVVKIVRIDPLTGLMGPVG
                     TNQDKSYKFFAEKFSGKGNPAGVTFEFRTIDNKLSPTESLNALKSALDSGVRYIAQGN
                     GSSVTAALLDAVNKHNERNPGKEVLLLNDASVDPDFTNSKCSYWHFRFDADTSMKMEA
                     ITTFMKNKPDIKKVYLLNQNYSHGHQVVKYAKEGLAKKRPDVQIVGEDLHPLAQVRDF
                     APYIAKIKASGADTVITGNWGSDLSLLVKAANEAGYEGKFFTYYAGVTGTPSALGQGA
                     AGRVYQVSYLHSNMGGQMQQWMNEFKAKHNDDFYTGSIPRIYAMLGAAMAQAKSTDPL
                     KVAAALEGLKVNTYNGEIEMRRLDHQLQQPLYVSVWQKTDAKHTYSPENTGMTFVPQA
                     EYPNYVSSTPTSCQMKRPGK"
     gene            13079..14101
                     /locus_tag="PKN51_07825"
     CDS             13079..14101
                     /locus_tag="PKN51_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT85045.1"
                     /translation="MEFFVISLLNGLSYGLLLFMLSSGLTLIFSMMGVLNFAHASFYM
                     LGAYLGYSIARVTGFWVALLLAPLVVGALGALFERLALRRVHRFGHVPELLITFGLSY
                     VILELVQLIWGRLALEFHPPELLKGPAFTIIKDAAGSLGLTWGAAPAQACQAAGTVCS
                     PFPATRAFMMLSAVVMLVSVWLLLTRTRIGLVIQAALTHPQAVETLGHNVPRVFMLVF
                     GAGCALAGLAGVVGGMTFLTEPAMAATVGSIIFVVVVVGGMGSLAGAFVASLIIGVIQ
                     TFAVALDHSLGSVLAQWGMTLSPTLQGNTLFKMTISQVAPILPYLFLVLILIFRPRGL
                     LGTREG"
     gene            14108..15394
                     /locus_tag="PKN51_07830"
     CDS             14108..15394
                     /locus_tag="PKN51_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT85046.1"
                     /translation="MTASSRSSASRARWLTWSGFALLLIVAPLVLSSGLSITILSQIG
                     IAIIVCLSYNMLLGQGGMLSFGHAVYSGLGSYLAIHALNAASAGRLPIPVSLIPLVGG
                     LAGLLFAVLLGYVTTKKSGTTFAMITLGVGELVFAMSLMVPEFFGGEGGVSGNRVMGQ
                     PFMGISFGPPIQVYYLIAFYTFLSVAGMYAFTRTPLGRMLNAVRDNPERVEFVGYNAQ
                     HVRYLAFIVAGFFAGVAGGLGALNFEIVTAEVVGAARSGAYLLFTFLGGATFFFGPII
                     GAVLMVLAFVLFSELTKAWLLYLGLIFMFMVMYAPGGIASLIMMNVRVARFGRLGGLI
                     PHYVVLTLAAIVTLAGASALIEMMYQVQLEGSDAALHFLGVTLQPAQTLHWVLAAAVM
                     LAGGALFEWARRRFARQWGSVQEHIEARIKQLEASA"
     gene            15391..16164
                     /locus_tag="PKN51_07835"
     CDS             15391..16164
                     /locus_tag="PKN51_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85047.1"
                     /translation="MSVPALQLKDVRKSFGKTEIIRGVSMSVQPGERVGVIGPNGAGK
                     STLFNLISGRFAPSSGEILLKGQRIDGRKPYEINRQGLSRSFQITNIFPKLSVFENLR
                     CGVLWSLGYRYTFLRFLASLDDANQRAEQLMRSIHLERKRDTLAMNLTYAEQRALEIG
                     ITIAGGADVILLDEPTAGMSRSETRHFIELIKEVTVGKTLLTVEHDMGVVFGLADKIA
                     VVVYGEVLAFDTPEAVRANARVQEAYLGSVVAQQQEQGH"
     gene            16167..16862
                     /locus_tag="PKN51_07840"
     CDS             16167..16862
                     /locus_tag="PKN51_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85048.1"
                     /translation="MLSVRDLHAFYGKSHVLHGVHFDVQPGEIVALLGRNGSGRSTTA
                     KAVMGMVQAQGDVRFKDQPLLGMKPFQIAHRGLGYVPESRDIFPTLTVQQNLLLGQKG
                     AGKGSRWTFDDMYAMFPRLKEREHTEAGVLSGGEQQMLTLCRTLMGDPELIIIDEPTE
                     GLAPKIVELVGEYLKKLKERGVSVLLIEQKLTIAMTISDRVLVMGHGSIVFEGSPAAL
                     RADAAVRKEWLEV"
     gene            17160..>18678
                     /locus_tag="PKN51_07845"
     CDS             17160..>18678
                     /locus_tag="PKN51_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNT85049.1"
                     /translation="MSADYQLHGDVAVITLNNPPVNGLGYATRVGITNGLERANADPA
                     VKAIVITGAGKAFSGGADIREFGTPKALQEPNLLSVILAIEHSAKPVVAAVHSVAMGG
                     GLELALGCHYRVAAPGAQIALPEVKLGLLPGAGGTQRLPRVLGVETALNMIVKGDPVS
                     SEVLASLPGQKLFDQMAASAESLLQEALAFAAQAAARQAAGEALPLVRQLPCKHPQGD
                     AYFQFARNMVKGMAKNFPAPAKCVDAVQAATKNKFDAGMAFERAIFVELMNTPESAAL
                     RHLFLAERAASKIPDVGDDVKPRAIQKVAVIGAGTMGGGISMNFLNAGIPVTILETKQ
                     EALDRGLATIRKNYEAQVKKGKLKQDKYEQRMALLTPTLSYDDLRDADLVIEAVFEEM
                     GVKEQVFKKLDEVVKPGAILASNTSTLDVDKIAAFTKRPQDVVGMHFFSPANVMKLLE
                     VVRGAKTAKDVLATVMQIAKKIRKTAVVSGVCDGFIGNRMIEQYSRQAGFLLEEGA"
BASE COUNT         3238 a         6096 c         6322 g         3022 t
ORIGIN      
        1 tcatcgacaa cgccgccgac gaggcgctgg ccggctacgg caagaagatc cgcgtgaccc
       61 tgcacgccga cgcctcggtc agcgtggaag acgacggccg cggcatcccc tacggcctgc
      121 accccgaaga aaaagccccg gtgctggagc tggtgttcac ccgactgcac gcaggcggca
      181 agttcgacaa gggcgcgggc ggtgcctaca gcttctcggg cggcctgcac ggcgtgggcg
      241 tgtcggtcac caacgccctg ggcaagcgca tggaggtgac cagccaccgc gagggcatgg
      301 tggccacgct ggccttcgag ggcggcgacg tggtgcaaaa gctgcacctg cgcaagcagg
      361 ccgagggtga ccgcaaacag ggcaccacgg tgcgcgtgtg gcccgacccc aagtacttcg
      421 aatcgcccca gctgcccatg gccgagctgg tgcacctgct tcgcagcaag gcggtgctga
      481 tgcccggcgt cacggtgcag ctgatcgacg agaagaagaa agacacgcaa agctggcagt
      541 acaagggcgg cctgcgcgac tacctggagc agaccttgcc cgccgacgcg gtgatcccgg
      601 tgttcgaagg tgagggcttt gccggcaagg acgacggcag ctttgccgaa ggcgagggcg
      661 cgcaatgggc cgtggccttc accgaagacg gcgcgccggt gcgcgagagc tacgtcaact
      721 tgatccccac cagcgccggt ggcacgcacg acagcggcct gcgcgacggc ctgtttcagg
      781 ccgtcaaggg cttcattgag ctgcacgcgc tgctgcccaa gggcgtcaag ctgatgcccg
      841 aagacgtgtt tgcgcgcgcc agctacgtgc tgtcggccaa ggtgctggac ccgcagtttc
      901 agggccagat caaggagcgg ctcaactcgc gcgacgcggt gcggctggtg tccggcttcg
      961 tcaagccggc gctggagctg tggctgaatc agcacgtgga atggggcaag cgcctggccg
     1021 agctggccat caaggccgcg caaacgcgcc agcgcgccgg gcaaaaggtc gaaaagcgca
     1081 agggcagcgg cgtggccgtg ctgcccggca agctgaccga ctgcgaaagc cgcgatctga
     1141 cggtcaacga agtgttcctg gtcgaaggcg acagcgccgg cggtagcgcc aagatggggc
     1201 gcgacaagga aacccaggcc atcctgccgc tgcgcggcaa ggtgctcaac acctgggagg
     1261 tcgagcgcga ccgcctgttt gccaataccg agattcacga catctcggtg gccatcggcg
     1321 tcgatccgca cgggccgcag gacgaggtcg atctgtccaa cctgcgttac ggcaaggtct
     1381 gtatcctgag tgatgccgac gtggacggcg cgcacatcca ggtgctgctg ctgaccctgt
     1441 ttttccgcca cttccccaag ctgatcgaag ccggccatgt gtacgtggcg cgcccaccct
     1501 tgtaccgcgt ggacgccccc gcgcgcggca aaaaacccgc cgccaagctg tacgcgctgg
     1561 atgagggcga gctggccgcc atccttgaca agctgcgcaa ggacggcgtg gccgaaggca
     1621 aatggaccat cagccgcttc aagggcctgg gcgagatgaa cgccgagcag ctgtgggaca
     1681 ccacgctcaa ccccgacacg cgccggctca tgccggtgca gctgggcgcc ttcgacttcg
     1741 ccgccaccgc cgccgagatc accaagctca tgggcaaggg cgaagccgcc gcccggcgcg
     1801 agctgatgga gctgcacggg gacgcggtgg aggtggatgt gtgatgggtg tgcggactct
     1861 tcgcgtcaat gtgtatatgt tcaagatata caatgacggc gtttgactgg acaaacatca
     1921 ccggctttga ctgggacgcc ggcaatgcac gcaagaatga caagcacggc gtctcgatgg
     1981 ccgaggccga gcaggtcttc ttcaaccaac cgctgctggt cgtgagcgat gacaagcaca
     2041 gccagaagga agcgcggctc catgcactgg gacgcacgga cggcgggcgg ctgctgcacc
     2101 tgacgttcac gctgcgccag cagggcagtc tgatacgcgt gatctcggcc agggccatgc
     2161 accgcaagga gcggacgatc tatgagcaag ccgaccaaga aggttccgca cctcggcagt
     2221 gaggcgcagg agcgtgcatt ttgggaaact cacgactcga cggactacgt cgattggtcc
     2281 aaggcccagc gtgtcgttct gcccaatctg aagccgacca ccaagaccat ctcgctgcgt
     2341 ctgccgcaac acctgctgga ctcgatcaag gccgcggcca acgcgcgcga cgtgccttat
     2401 cagtcgctga tcaaggtctg gttgcaggac aagctgcgcg gctgaaggcg tggatggcga
     2461 tgggagcgca aacgttgaga tcgcacgttt tgatgccata attgaatcca tatggcatca
     2521 atttcgatgc atttcgccgg agtctttcat gcgcaccacc gtcaccatcg aagacacgct
     2581 gtacgaccag gcgctcgaac tggccgaccc cgacatggac aaggccgagc tgtttcgcga
     2641 agccatccgc accttcatcc gcgtgcagtc cggcaagcgg ctggcccgct tgggcgggca
     2701 ggcgccgcaa atggccgagg tgccgcgtcg gcgcgccccc gctcgagcgc ccgccgcgtg
     2761 acgcccgcgc ccgaagtgct ggtcgacacc agcgtctgga tcgagcactt tcgccgcggc
     2821 agcgcgccgc tggccgagct gctggcgggc gatgccgtgc gcatgcaccc gctggtgctg
     2881 ctggagctgg cctgcggcag ccccccggca ccgcgcgcgc gcaccctggc cgacctggcc
     2941 cgcctgcgcc cggccccaca ggccggccag gccgagctgc tggcgttcat cgagcaggag
     3001 caactgttcg acagcggctg cggcgccgtc gatatccacc tgctggccag cgcccgcctg
     3061 ctgcccggcg cgcggctgtg gacgctggac aaaccgctgc acgcgctggc ccggcggctg
     3121 ggtgtggcgt gggtggtgcc gacccgatga gtacgtgggt agcgcgcgcc tgcactgagc
     3181 tgcagcgcat agcgtgccag tcgattcaat tttgtatgta gaccatgtcc gaccaaaccg
     3241 ttctcgacct ttcccccgcc gaccccggcg acgacgtggc gctggccacc tacgcccagc
     3301 gcgcctacct cgaatacgcc ttgtccgtgg tcaagggccg cgcgctgccc gatgtgtgcg
     3361 acgggcaaaa gccggtgcag cgacgcatcc tgtacgcgat ggagcgcatg ggcctgggct
     3421 atggtggccc ggcgggcaac accgcggcca agccggtcaa gagcgcgcgc gtggtgggcg
     3481 acgtgctggg gcgctttcac ccgcacggcg acagcgccgc ctacgaggcg ctggtgcgca
     3541 tggcgcagga cttttcgcag cgctatccat tgatcgacgg gcagggcaac ttcggcagcc
     3601 gcgacggcga cggcgcggct gccatgcgct acaccgaggc gcggctggcc aggatcacgc
     3661 gcctgctgct ctcggagatc gaccagggca cggtggactg ggtgcccaat tacgacggct
     3721 cgaccgaaga gccgcgccag ctgccggcgc gcctcccgtt cgcgctgctc aacggcgcca
     3781 gcggcatcgc cgtcggcctg gccaccgaga tccccagcca caacctgcgc gaggtggccg
     3841 atgcctgcat cgccctggtc aagaacccga agctggcgga cgacgagctg tttgccctgc
     3901 tgccggggcc ggactacccc ggtggcggcc agatcatcag cagcgacagc gagatccgcg
     3961 ccgcttatca gagcgggcgc ggcagcctca aggtgcgcgc gcgctgggtc atcgaagacc
     4021 tggcgcgcgg ccagtggcag ctggtggtga ccgagctgcc gccgggcacc tcggcgcaga
     4081 aggtgctcga agaaatcgag gagctcacca accccaaggt caagaccggc aagaaggcgg
     4141 tgacgcagga gcagaaccag ctcaagggca cgatcctcgc ggtgctcgac ggcgtgcgcg
     4201 acgaatcgag caaggatgcg ccggtgcgcc tggtgttcga gcccaagagc cgcaccattg
     4261 gccaggacga gctggccaac acgctgctgg cgcacaccag cctggagtgc agcagcccga
     4321 tcaacctgac cagcgtgggg ctggacgggc ggccggtgca aaaaagcctg cgccagatgc
     4381 tggccgagtg gatcgagttt cgccagagca cgatcacgcg gcgcagccag catcgcttgc
     4441 agaaggtgct ggagcgcatc cacatcctcg agggccggca gctggtgctg ctcaacatcg
     4501 acgaggtgat cgccatcatc cgcacggccg acgagcccaa ggcggcgctg atcgcgcgct
     4561 ttgccctgac cgagcggcag gccgacgaca tcctggacat ccgcttgcgc cagctggccc
     4621 ggctggaggc catcaagatc gagcaggaac tcagtgaact gcgcaaggag cagggcagcc
     4681 tggaggacgt tttggccagc cccgccacgc tgcgccgcct gatgatcaaa gagatcgaag
     4741 ctgacgccaa agagtttgcc gacccccgcc gcaccctgat ccaggccgag aaaaaggccg
     4801 tggtcgaagt gaaagtgcag gacgagccgg tcacggtggt ggtcagcgaa aagggctggg
     4861 tgcgcgcgcg cagcggccat gggcacgacg cggcgggttt tgccttcaag gccggtgacg
     4921 gcctgtacgg cacgttcgaa tgccgcacgg tcgaccagct gctggtgttt ggcagcaacg
     4981 ggcgcgtcta cagcgtggcg gtggccagcc tgccgggcgc acgcggcgat ggccagccga
     5041 tcaccaccct gatcgagctg gaagcgggta cccagccgct gcactacttt gccgggccgg
     5101 cgggcgccgc gctgctgctg tcgggcagtg gcggctacgg ctttgtggcg cgggtggagc
     5161 acatgctgtc gcgcaacaag agtggcaagg ccttcatcag catcggcgcc ggcgagcagg
     5221 tgtgcaggcc ctcgctggtc gcgctggatc cagagcaaaa aacggctccg acgcagactg
     5281 gacaagcgcc agcagctatt ggatcaatag cagccacaca cgtggcctgt gcctcggtgg
     5341 gtgggcgcat cctgaccttt gatatcgggg agctgaaact gatggagaag ggcgggcgtg
     5401 gcctcaccct gatcgacctg gagcccaagg acagcctggc cggcgccgca gcctacacgc
     5461 gcagcgtgcg cctgctgggc gtcggccgcg gcggcaaacc gcgtgaggag caactggaga
     5521 tccgcagcct gaacaacgcc cgcgccgcgc gcgggcgcaa gggcagggcc gccgacctgg
     5581 gcttcaagcc ccacaccatc gtgcgggtgg agtgatgggt ggcctggatc tttctcccct
     5641 gggcatcgtg ctgctggtgg tgaccggcgt gctcagcttt gcgctggggc gctggtggtc
     5701 gcgcggctgg cgcgcccgac gccaggcgcg ccgggcggcc gaggcgcgca agctcgaaac
     5761 acgccagcag cgccgtgccc gcgagcgcga gcaggccaag cgccgatgac gcgaacgcgc
     5821 gttcaacctg gaaacggccg ttggacgagc ccgcccgtgc cgtgatcggt gtgccaggca
     5881 agggtgagcg ccgcggctcc aaaggcgcct gcgcgccttg ggacggcgcg aacgggccca
     5941 gcctctggct gggctgcgcc ctgcaaggcc gacgccgccg cgggctcctg cccgcaagaa
     6001 ggagcaacgc cgcatggcgc gccgagcgcg gtgcgggtgg gtgcgcagcg tgctcaccca
     6061 ggaggtgaag gccatcgcag ccgccagacc ccgcatcgat cgatgcggcc tgcccgaagt
     6121 tgaccagcgg tcaaatgccg tttccaggtt caataggccc aaacgctgga tggcctcgct
     6181 gcgcttcgat gccccatggg tcaggggtca tgctttttga acgcgacgcc gtatgaggcg
     6241 caaaaaaggg gcggccgcct gaagcgaccg cccccttggg caagcggcaa ggcgcggctt
     6301 actcgacctt ggccttggtg cgcaggtcct gctggaactg caccagcttc tgctgctgca
     6361 gctgctgcac gatctgcggc ttgacggctt cgaagctggg cagctcgggc gccttggcct
     6421 cgcgcaggtc gtcgacgcgg atcacgtgcc agccgaactg cgacttgaca ggagcatcgg
     6481 tcagttgccc cttggccagc ttgcccagcg catcggcgaa ctcgggcacg taaccggacg
     6541 ggttggccca gcccagatcg ccgccctggg cgccggagcc ggggtccttg gactgcttct
     6601 tggccagctc ctcgaacttg gcgcccttct tgagttgggc gatgatggcc ttggcctcgt
     6661 cctccttctc gaccaggatg tggcgggcct tgatctcctt ggcgccggcc gccggcacct
     6721 ggctgccgat gaccttgtcg tactcggcct tggcctcggc gtcgctcacc gggttgtttt
     6781 tctggaaatc ggcgaacagc tcgccgatca gcacgctctg gcgcgccagg tccatcttgg
     6841 cgcggtagtc gggcgaggct tccaggccgc ggcgctgggc ctcctgcatg aagacctcac
     6901 gggcgatgat ttcttcgcgc agctgacctt gcacctcggg cgggatcggc tggccggtgc
     6961 gggcggcctg ggccttgatc tgcgcctcca gcgcgtccag gcgcgccttg ggcaccggct
     7021 tgccgttgac gatggccacg ttctgggcct gggccggcag gctcagggca ccggtcagca
     7081 cggccgccgc ggcggcggcc agaagggtct tgctcatgtg gatgaaacct cgaagacgcc
     7141 ccggggcggg gcaggggaag aaaaccgtga attatcgcgc ctcgatggcc agcgcatgca
     7201 cgccgtcgcg ctcgatgaaa tcctgtaacg cctgatacac cagctggtgg cgcaccacgc
     7261 ggctcttgcc gtcgaacagc ggcgaggcga tgcgcacgcg aaaatgggtg ccgaagccgg
     7321 tgccgttggc gccggcgtgg ccctcgtgct gccagctttc gtccagcact tcaagggcgc
     7381 tgggttgcag tcgttcgcgc aggcgcgcgg ccatgcggtc ggccagttcg gtgctcatca
     7441 ggtgctttcc ggcgggttgc ccgccgcggg ttcgtcgctc aggtgacggg ccacgtagat
     7501 gccctggccg acgatgaagg cgaccggaaa cacgtagccc cacagcttga agtcggccca
     7561 ggcgtcggtg ctccagaagg ccgccacgta gccgttgagc gtggccatga acaggcagta
     7621 cagcacccag gccagcgtca ggcggtgcca caccggttcg ggcaggctga gctggctgcc
     7681 cagcatgatc ttgagaaagt tcttgcggta ggcccacagc gccaccgcca gcgccagcgc
     7741 catggcggca tacaggaccg tcggcttcca tttgatgaag cgctcgtcct gcagcaccag
     7801 ggtcagcgtg ccgaagacca ggatcaaggc cagggtggcc ttgtgcatgg ccgtgagctt
     7861 cctgtcgatg gcgtagatga tgcccatctg cacgcaggtg gcggccatca gcacggcggt
     7921 ggccgggtag atgccataca gcttgtaggc accgaagaac agcaggatgg gcaggaagtc
     7981 gagcagggct ttcatgcagg cgcgcagttt accggggctc gaagtccagc gcggccgagt
     8041 tcatgcagta gcgcagcccg gtgggtgccg ggccgtcggg gaacacatgg cccaggtggg
     8101 cgccgcaccg ggcgcacagg gtttcgacgc gcaccatgcc gtggctggtg tcgcgccgct
     8161 cctcgatggc gccgggcagg gcggcctgcc agaagctggg ccagccgcag ccggcgtcga
     8221 acttggtgtc ggactcgaac agcctggcgc cgcagcagat gcagtggtag ctgccatcct
     8281 gccaatggcg ctcgtacctg cccgaaaacg ggcgttcggt ggcggcgtgg cgcgtgacct
     8341 ggaaggccag tggctcggcc cctttgtcgg ccagcagggc acgccactcg gcgtcggatt
     8401 tctgaacggg atggctcatg aggaacagct gatctggatc tgtgatgccc agtcgggcgg
     8461 aaagttgcac cagtcggcgt gctcgggctg ctcgtcgaac gggcgctgca gcgcgtcgtg
     8521 caaggactgc accatgctga agtcgtgctg ccgggccagc gcaatggcct gctcggccag
     8581 gtggttgcgc agcaccaggc cgggattggt tttcagcatc aaatcggcag tttgcgccct
     8641 attggattgc gccagcagct cctgatattg tagcaaaagg cgatcgatgg cggcccgatc
     8701 gtgaaacaaa tccgtcaccg gctggcgcag gtcaccggcc acctgctgcg ccaggcggcg
     8761 ccagaagatg gtccagtcgg tgcgctcgcg cgtcagcagc tcgagcagcg cctgcaccag
     8821 ctggcgcgag ccgtcgctgg cctgcggcag gccgagcttg agcgccatgc ggcggtccag
     8881 ctcggcactg aacagcgccg ggaagggctg cagcaccgcc agcgccgcat cgggctgtcg
     8941 aatcagcggc agcagcgcct gaccgagggc aaacaggttc cagtgggcga tctcgggctg
     9001 gcgctggtag gcgtagcgcc ccaggtggtc ggaatggttg cagatgcggg ccgggtcgaa
     9061 gccgtccatg aactgaaacg gcccgtagtc gatggtcagg cccaggatgc tcatgttgtc
     9121 cgtgttcatc acgccatgca tgaagcccag cgcctgccac tgtgccagca gcgccgcggt
     9181 gcgctgcgtc accgcgccca gcagcgccgc gtaggggttg ctggcgtcgc ggcagtcggg
     9241 gtagaagcgc gcgatgacga agtccgccag cgtgcgcagc gcttcgtgcc gaccgctgtg
     9301 gcaaaagtgc tcgaaatgcc cgaagcggat gaagctgggg gcggcgcgca gcaccacggc
     9361 ggcggtttcc aggcgttcac gtcgcaccgg ctcgtccgag ccggcgacgc acagcgcccg
     9421 cgtggtcggc acgcccagcg catgcatggc ctcgctgcac aggaactcgc ggatcgacga
     9481 gcgcagcacc gcgcggccgt cgcccatgcg cgagtagggc gtcaggccac tgcctttgag
     9541 ctgcaactcc tgcgagccac cgccagtcgt gcgcacttcg cccagcagga tggcgcggcc
     9601 gtcgcccagt tgcccggccc aggcgccgaa ctggtggccg ccgtagacgc tggccagcgg
     9661 cgcgctgccg gccagcggtt ggccgccgct gacggccggc aaggcgccct ggacctgcca
     9721 ccagtcgtcg gccaggccga gttcacgcgc catggcggcg ctgcgggcca cccagtacgc
     9781 ctcgccacgc agcggcagcg gcgccaggcg ggtgaaaaaa ccctcgccca ggcgttcgaa
     9841 ggcgttgtcc caggtcaggc ccagatcgac cgcttggacg gagtcggtcc tgttcagcat
     9901 caagccattg tcacttgcgt ggcacgagcg tgcagaaacc ccagggtggt gaattagtat
     9961 tgcatctgct atcgcaaccg ctcgcacaaa gaggagaacc atgctgggcc agatgcagaa
    10021 ccaccccttg ctgacttcca tgctgctgga gttcgccgag cgccaccacg gcgacggcga
    10081 aatcgtttcc cgcagggtcg agggcgacat ccatcgctac acctaccgcg acctggccag
    10141 gcgctcgcgc cagttggccg ccacgctcga caaagcgggg ctccagcagg gcgaccgcgt
    10201 ggccacgctg gcgtggaacg gctaccggca cctggagctc tacttcgggg tcaccggctc
    10261 cgggcgcgtc gttcacacgc tcaatccgcg cctgcatccg gagcagctgg tgtggatcat
    10321 caaccacgcc gaagacgccg tgctgtgctt cgacatgacg ttcctgcccc tgatccaggc
    10381 cgtgcatgcc aagtgcccgt cggtcaagac ctgggtcgcc ctgtgcgagg ccgacaagct
    10441 gccggccgac agcggcattc cccagctggt cagctacgag gcctggatcg gcacctcgac
    10501 cgaccagtat gtctggccca agttcgacga aaacagcgcc gccggcctgt gctacaccag
    10561 cggcaccacc ggcaacccca agggcgccct gtacagccac cgctcgaccg tgctgcacgc
    10621 ctacggtggc gcactgcccg atgcgctcaa cctgtcggcg cgcgactcga tgatgccggt
    10681 ggtgcccatg ttccacgtca acgcctgggg cctgccctat gtcgcggcgt tgaccggctg
    10741 caaggtggtg tttccggggc cggcgatgga cggcaagtcg atctacgagc tgatggaggc
    10801 cgagggcgtc agcatggccg ccggcgtgcc caccatctgg cagatgctgc tggggcatgt
    10861 gcagggcaac aagctcaagt tctccaccct caaccgcacc gtgatcggcg gctcggcctg
    10921 cccaccggcc atgatcgaca ccttccgcga ggagttcggc gtcgacgtgc tgcatgcctg
    10981 gggcatgacc gaaaccagcc cgctgggttc ggtcagcacg ctcaagaaca agcacctcaa
    11041 gcagccgcgc gagcagcagc tggccattcg cgtcaagcag gggcgcgcca tctgcggcgt
    11101 ggacatgcgc atcgtcggcg acgacggaaa cgagcagccc tgggacggca agacgttcgg
    11161 tgacctgcag gtgcgtggcc cgtggatcat cgagtcctac ttcaagggcg agaccccact
    11221 ggtgaccgac gagcacggcc accgctggtt ccccaccggc gatgtggcga ccatcgatgc
    11281 cgacggctac atgcagatca ccgaccgcag caaggacgtc atcaagtccg gcggtgagtg
    11341 gatcagctcg atcgacatcg aaaacatcgc catggcgcac cccgcgatcg ccatggccgc
    11401 ctgcatcggc atgcctcacc ccaagtggga cgagcgcccg atcgtcgccg tggtcaggaa
    11461 gcccggcgcc gaggtgacgc gcgaggagct gctggcgttc tacgaaggca agaccgccaa
    11521 gtggcagatt ccggacgacg tggtgttcgt cgacgccatc ccgctgggcg ccaccggcaa
    11581 gatgctcaag acccgcctgc gcgagctgct caaggactac aagctgccga ccgcctgacg
    11641 cacggcgcta cccgcctggc acttgcgcga caacgggttg gggcaaaccc ggctgcagtg
    11701 tgccgacagc aaagctacgc ttacggccgt ttcaatccaa ggagacatca gatgaagttc
    11761 gtttggaaat ccgtcatgac cgcctcggcg ctgctgctgg gcggcgccgc cctggcgcag
    11821 cagggcgagg tggtgaagat cgtgcgcatc gacccgctga ccggcctgat gggtccggtg
    11881 ggcaccaacc aggacaagag ttacaaattc ttcgccgaaa agttcagcgg caagggcaac
    11941 ccggccggcg tgaccttcga gttccgcacc atcgacaaca agctgagccc gaccgaaagc
    12001 ctgaacgcgc tgaagtcggc gctcgactcc ggcgtgcgct acatcgctca gggcaatggc
    12061 tcctcggtca cggcggccct gctggacgcc gtcaacaagc acaacgagcg caacccgggc
    12121 aaggaagtgc tgctgctgaa cgacgcctcc gtcgacccgg atttcaccaa cagcaaatgc
    12181 agctactggc acttccgctt cgacgccgac acttcgatga agatggaggc catcaccacc
    12241 ttcatgaaga acaagcccga catcaagaag gtgtacctgc tgaaccagaa ctactcgcac
    12301 ggtcaccagg tggtcaagta cgccaaggaa ggtctggcca agaagcgccc cgacgtgcag
    12361 atcgtcggcg aagatctgca cccgctggcg caggtgcgcg acttcgcgcc ctacatcgcg
    12421 aagatcaagg cttcgggcgc cgacacggtg atcaccggca actggggttc cgacctgtcg
    12481 ctgctggtca aggccgccaa cgaagccggc tacgagggca agttcttcac ctactacgcc
    12541 ggcgtcacgg gcacaccgtc cgctttgggg cagggtgctg ccgggcgggt gtatcaggtc
    12601 tcctacctcc attccaacat gggtggccag atgcagcagt ggatgaacga gttcaaggcc
    12661 aagcacaacg atgacttcta caccggctcg atcccacgca tctacgccat gctgggcgct
    12721 gccatggcgc aggccaagtc caccgatcct ttgaaggtgg cggcggccct ggaagggctc
    12781 aaggtcaaca cctacaacgg cgagatcgag atgcggcggc tggatcacca gctgcagcaa
    12841 cccctgtatg tctcggtctg gcagaagacc gacgccaagc acacctacag cccggagaac
    12901 accggcatga ccttcgtgcc tcaggccgaa taccccaact acgtctccag cacgccgacc
    12961 agctgccaga tgaagcggcc cggcaagtga cgacacaggc ggcctgaagt cgccgccgtg
    13021 actgcgaagg cccgaacggg ttgccgtctc gggccttcat tttggagaat tgccggccat
    13081 ggaatttttc gtcatctcat tgctcaacgg cctgagctac ggtctgctgc tgttcatgct
    13141 cagctccggt ctgacgctga tcttcagcat gatgggggtg ctcaatttcg cccacgccag
    13201 cttctacatg ctgggcgcct atctgggcta cagcatcgcg cgcgtcaccg gcttctgggt
    13261 ggcactgctg ctggcaccgc tggtggtcgg cgccctgggc gcgctgttcg agcgcctggc
    13321 gctgcggcgg gtgcaccgct tcggccatgt gccggagctg ctgatcacct tcgggctgtc
    13381 ctatgtgatc ctcgaactgg tgcaactgat ctggggccgg ctggcgctcg aattccatcc
    13441 gcccgagctg ctgaaaggcc ccgcattcac catcatcaag gacgctgccg gcagcctggg
    13501 cctgacctgg ggggcggcgc cggcgcaagc ctgtcaggcg gcgggcacgg tctgttcgcc
    13561 gttcccggcc acgcgcgcct tcatgatgct gtcggcggtg gtgatgctgg tgtcggtgtg
    13621 gctgctgctg acacgcaccc gcatcggcct ggtgatccag gccgcgctga cgcacccgca
    13681 ggcggtggag accctgggcc acaacgtgcc gcgcgtgttc atgctggtgt ttggtgcggg
    13741 ctgcgcgctg gccggcctgg ccggtgtggt ggggggcatg acctttctca ccgagccggc
    13801 gatggcggcc accgtgggct cgatcatctt cgtggtggtg gtggtgggcg gcatgggctc
    13861 gctggccggt gcgttcgtgg cttcgctgat catcggtgtg atccagacct ttgccgttgc
    13921 cctggaccat tcgctcggct ccgtgctggc gcaatggggc atgacgctca gtccaacgct
    13981 gcagggcaac acgctgttca agatgacgat ctcgcaggtg gcacccatcc tgccgtatct
    14041 gttcctggtg ctgatcctga tcttcaggcc ccgggggttg ttgggcacac gcgagggttg
    14101 acgcaccatg accgcttcgt cacgttcctc ggccagccgg gcgcgctggc tcacctggtc
    14161 cggttttgcg ctgctgctga tcgtggcgcc gctggtgctg tccagcggcc tgtccatcac
    14221 catcctgtcg cagatcggca tcgccatcat cgtctgcctg tcctacaaca tgttgctggg
    14281 gcagggcggc atgctcagct tcggccacgc ggtgtattcg ggcctgggtt cctatctggc
    14341 catccacgcg ctcaatgcag cctcggcggg ccgcctgccg attccggtgt cgctgattcc
    14401 gctggtgggc ggtctggcgg gccttctgtt tgcggtgctg ctgggctatg tgaccaccaa
    14461 gaagtccggc accaccttcg ccatgatcac gctgggtgtg ggcgaactgg tgttcgccat
    14521 gtcgctgatg gtgccggagt tcttcggtgg cgagggcggc gtgtcgggca atcgggtgat
    14581 ggggcagccg ttcatgggca tcagtttcgg tccaccgatt caggtctatt acctgatcgc
    14641 gttctacacc ttcctgagcg tcgccggcat gtatgccttc acgcgcacac cgctggggcg
    14701 catgctcaac gccgtacgtg acaacccgga acgggtcgag ttcgtcggct acaacgccca
    14761 gcacgtgcgc tatctggcgt tcatcgtggc cggcttcttt gccggtgtcg ccggtggcct
    14821 gggggcgctc aacttcgaga tcgtcaccgc cgaggtggtg ggcgccgcgc gctcgggcgc
    14881 ctacctgctg ttcaccttcc tgggtggcgc caccttcttc ttcggcccca tcatcggcgc
    14941 ggtgctgatg gtgctggcct tcgtgctgtt ttccgaactc accaaggcct ggctgctgta
    15001 cctgggcctg atcttcatgt tcatggtgat gtatgccccg ggcggcatcg ccagcctgat
    15061 catgatgaac gtgcgggtgg cgcgcttcgg gcggctgggg gggctgatcc cgcactatgt
    15121 ggtgctgacg ctggccgcca tcgtgacgct ggcaggcgcc tcggccttga tcgaaatgat
    15181 gtatcaggtg caacttgaag gctccgacgc ggcgctgcac tttctggggg tgacgctgca
    15241 gccggcgcag accctgcact gggtgctggc tgccgcggtg atgctggcgg gtggtgcgct
    15301 gttcgagtgg gcgcgccgcc gcttcgcgcg ccagtggggc tcggtccagg aacacatcga
    15361 agcgcgcatc aagcaactgg aggcttcggc atgagcgtgc ccgccctgca actgaaggac
    15421 gtgcgcaaga gtttcggcaa gaccgagatc attcgcggcg tcagcatgag cgtgcagcct
    15481 ggtgaacgcg tgggcgtgat cgggcccaac ggtgcgggca agtcgacgct gttcaacctg
    15541 atcagcggcc gctttgcgcc cagcagcggc gagatcctgc tcaaagggca gcgcatcgac
    15601 ggtcgcaagc cctacgagat caaccgccag ggcctgtcgc gcagcttcca gatcaccaac
    15661 atctttccca agctgagcgt gttcgagaac ctgcgctgcg gtgtgttgtg gagcctgggt
    15721 taccgttaca cgttcctgcg ctttctggcc agcctggacg acgccaacca gcgcgccgag
    15781 cagctcatgc gcagcatcca cctggagcgc aagcgtgaca ccctggcaat gaacctgacc
    15841 tacgccgagc aacgcgcgct ggagatcggc atcaccatcg ccggcggggc cgacgtgatc
    15901 ctgctggacg agcccaccgc cggcatgagc cgcagcgaaa cgcgccattt catcgaactg
    15961 atcaaggagg tgacggtggg caagaccttg ctcacggtcg agcacgacat gggggtggtg
    16021 ttcggcctgg ccgacaagat cgcggtggtg gtgtacggcg aggtgctggc gttcgacacc
    16081 cccgaagcgg tgcgcgccaa cgcgcgcgtg caggaagcct atctgggctc ggtggtggcg
    16141 cagcagcagg aacaggggca ttgacgatgt tgagcgtgcg cgaccttcat gccttctacg
    16201 gcaagagcca tgtgctgcat ggcgtgcatt tcgatgtgca acccggcgag atcgtggcgc
    16261 tgctggggcg caacggttcg ggccgatcga ccacggccaa ggccgtgatg ggcatggtgc
    16321 aggcgcaggg tgatgtgcgt ttcaaggatc agccgctgct gggcatgaag ccgttccaga
    16381 tcgcccaccg gggcctgggc tatgtgccag aaagccgcga cattttcccc accctgacgg
    16441 tgcagcagaa cctgctgctg ggccagaagg gcgcaggcaa gggcagtcgc tggacgttcg
    16501 acgacatgta cgccatgttt ccgcgcctga aggagcgcga gcacaccgaa gccggcgtgc
    16561 tgtcgggcgg cgagcagcag atgctgaccc tgtgccgcac cctgatgggc gaccctgaac
    16621 tcatcatcat cgacgagccc accgaaggcc tggcgcccaa gatcgtcgaa ctggtgggcg
    16681 aatacctgaa gaagctgaag gagcgcggcg tgtccgtgct gctgatcgag cagaagctga
    16741 ccatcgctat gacgatctcc gatcgcgtgc tggtgatggg gcacggcagc atcgtgttcg
    16801 aaggctcgcc tgctgcgctg cgcgccgacg cggcggtgcg caaggagtgg ctggaagtgt
    16861 gaagctcggg cgagggcagg gtggggcgcc acgctatcgg ttcaatagct gttggcgcat
    16921 gctccacctt gattctgaag cctttcgcca tgattttcct tgcgcagcaa gcgccgacag
    16981 ctatcgaacc tgaagcgcgc tgcgccttcg tctctcctgt gacaacccgg tcacgtcacc
    17041 atggcgcata ctatccacac ccgtctgatc gcggtcgtct ccgggcccgc caggagcgcc
    17101 gaaggccgcg acgtcaccat ctcacattcc cgcaatccgc gcacacctga ggagcccgca
    17161 tgagcgctga ctaccagctg cacggcgatg tcgccgtcat taccctgaac aacccgccgg
    17221 tcaacggcct gggctacgcc acgcgggtgg gcatcaccaa cggactggag cgtgccaacg
    17281 ccgacccggc ggtcaaggcc atcgtcatca ccggcgccgg caaggcgttt tccggcggtg
    17341 ccgacatccg tgaattcggc acgcccaagg ccttgcagga gcccaacctg ctttcggtca
    17401 tcctggccat cgaacactcg gccaagccgg tggtcgccgc cgtccacagc gtggccatgg
    17461 gcgggggcct ggagctggcc ctgggctgcc actaccgcgt ggcggcaccg ggcgcgcaga
    17521 ttgccttgcc cgaggtcaag ctgggcctgc tgccgggcgc cggcggcacc cagcgcctgc
    17581 cgcgcgtgct gggggtggag accgcgctca acatgatcgt caagggcgac ccggtgagca
    17641 gcgaagtgct ggccagcctg cctgggcaga agctgttcga tcagatggcc gccagcgcag
    17701 aatcgctgct gcaggaggcc ctggcgtttg ccgctcaggc cgccgcccgt caggcggcgg
    17761 gcgaagcgct gccgctggtg cgccagttgc cgtgcaagca cccgcagggc gacgcgtact
    17821 tccagttcgc gcgcaacatg gtcaagggca tggccaagaa cttcccggca cccgccaaat
    17881 gtgtcgacgc cgtgcaggcc gccaccaaga acaagttcga tgccggcatg gcgttcgagc
    17941 gggcgatctt cgtggaactg atgaacaccc cggaaagcgc tgccctgcgc cacctgttcc
    18001 tggccgagcg tgcggccagc aagattcccg acgtgggcga cgacgtcaaa ccgcgcgcca
    18061 tccagaaggt ggcggtgatc ggcgccggca ccatgggcgg cggcatatcg atgaacttcc
    18121 tcaacgccgg cattcccgtc accattctgg agaccaagca ggaagcgctg gaccgcggcc
    18181 tggccaccat ccgcaagaac tacgaggcgc aggtcaagaa gggcaaactc aagcaggaca
    18241 agtacgagca gcgcatggcc ctgctgaccc ccacgctgag ctacgacgac cttcgcgacg
    18301 ccgacctggt gatcgaggcc gtgttcgagg agatgggcgt caaggagcag gtgttcaaaa
    18361 agctcgacga agtggtcaaa cccggcgcca tcctggcgtc caacacctcc acgctcgacg
    18421 tcgacaagat cgccgccttc accaagcgcc cgcaggacgt ggtgggcatg cacttcttca
    18481 gcccggccaa cgtgatgaag ctgctcgaag tggtgcgcgg cgccaagacc gccaaggacg
    18541 tgctggccac ggtgatgcag atcgccaaga agatcaggaa gaccgcggtg gtctccggcg
    18601 tgtgcgacgg gttcatcggt aatcgcatga tcgagcagta cagccgccag gccggcttcc
    18661 tgctggaaga gggcgcca
//
LOCUS       DAOQTZ010000053        18158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3538_length_18158_cov_13.380545, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000053 DAOQTZ010000000
VERSION     DAOQTZ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 18158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18158
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3538_length_18158_cov_13.380545"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..625)
                     /locus_tag="PKN51_07850"
     CDS             complement(296..625)
                     /locus_tag="PKN51_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNT85050.1"
                     /translation="MTHVVTEACINCKYTDCVDVCPVDCFKEGPNFLTIDPDECIDCA
                     VCIPECPVNAIFAEEDLPKDQLHMTQINAELARSPNYRTLTKRKDPLPDHEAWASRTD
                     KLPELVR"
     gene            complement(987..2477)
                     /locus_tag="PKN51_07855"
     CDS             complement(987..2477)
                     /locus_tag="PKN51_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HNT85051.1"
                     /translation="MSTTAVHPVDERLPTGKLTALGLQHVLVMYAGAVAVPLIVGRAL
                     KLGPQEVAFLISADLFCCGIATLIQALGATQWFGIRLPVMMGVTFAAVGPMVAIANGN
                     PGTDGARMLFGAIIGAGIISILIAPVVSRMLRFFPPVVTGTIIAVIGINLMRVGINWI
                     FGNPVGPTAPLTLSPEHTEWLSTMRSAGATGQPGVLPVPQGLTLAPTVPNPNYAKLSG
                     VAVSAIVLASILLIAKFAKGFIANISVLLGIVIGGIVATATGMMNFDKVGKAAWFDVV
                     TPFHFGTPIFDPVMILTMTLIMIVVMIESTGMFLALGEMTDRRIDQPMLSRGLRTDGL
                     GTLIGGIFNTFPYTSFSQNVGLVAVTGVKSRFVCVAGGLILIVLGLLPKMAALVESLP
                     TVVLGGAGIVMFGMVAATGIRILGGVDYTHNRNNALIVAISIGVGMIPLVAPRYLQWM
                     PHAIHPLIESGIVLASIAAVLLNLFFNGARADTEASIRAARQAEAH"
     gene            complement(2529..3797)
                     /locus_tag="PKN51_07860"
     CDS             complement(2529..3797)
                     /locus_tag="PKN51_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT85052.1"
                     /translation="MTTATDTYARLDAWIDQHFDEQVRFLQQLVRVPTDTPPGNNAPH
                     AERTAELIAAWGFEAERHPVPEQEVRDYGMQSITNLIVRRPYGKGGRTIALNAHGDVV
                     PPGEGWTHDPYGAEIEGGQMFGRATAVSKSDFSTFSFAVRALEAVAKPTRGTVELHFT
                     YDEEFGGLLGPGWLLAQGLTRPDLMIAAGFSYEVVTAHNGCLQMEVTVQGRMAHAAVP
                     HTGVDALQAAVFIMNALYAENSAYQKVSSKVPGIRHPYLNIGRIEGGTNTNVIPGKVV
                     LKLDRRMIPEEKPAKVEARIRKVIEGATRDFNRWRGVKGEDAVRTDVRRILLAHAMTP
                     LPGNRPLVEAIQKHGEAVFGARPPAVGTPLYTDVRLYVERGIPGVIYGAGPRSVLESH
                     AKRADERVDLQDLRRATKVIARALADLLHN"
     gene            complement(3852..5642)
                     /gene="uraD"
                     /locus_tag="PKN51_07865"
     CDS             complement(3852..5642)
                     /gene="uraD"
                     /locus_tag="PKN51_07865"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HNT85053.1"
                     /translation="MPYTLDQLNAAPLAEAERMLDGLYEHTPWIAAQALQARPFQSLA
                     QLKQAMAGALTRASVDAQLALIRAHPELAGKAMEAGTLTSESTGEQQRAGLTACTPEE
                     LARIRQLNADYQARFGFPFVLCVRGPRGTGLAKQQILDTFERRLHHHPDFERAEALRQ
                     IHRIVEIRLADKFGAPPTLGEQVWDWHEKLAQHSDPGCAEAGQLTVTYLTDAHRACAQ
                     RISRNMRDAGFDDVHIDAVGNVVGRYKADPGARNPKTLLTGSHYDTVRNGGKYDGRLG
                     IFVPLACVQELARTGRRLPFDLEVVGFAEEEGQRYKATFLGSGALIGDFRPEWLDQRD
                     ADGISMREAMQHAGLCVDDIPKLKRHAADYLGFIEVHIEQGPVLNELNLPLGVVTSIN
                     GSVRYQGAYIGQASHAGTTPMDRRRDAACAAAELALYLERRAAQDGDSVGTIGMLTVP
                     NGSINVVPGRCNFSLDLRAPSDAQRDALVSDVLAEAQAIAERRGVRLQLEETMRAAAA
                     PCDAALQQRWADAVEALGLPVFRMPSGAGHDAMKLHEIMPQAMLFVRGENAGISHNPL
                     ESTSAEDMALAVHAMQHLLDGMARDTLSQS"
     gene            complement(5649..6605)
                     /gene="puuE"
                     /locus_tag="PKN51_07870"
     CDS             complement(5649..6605)
                     /gene="puuE"
                     /locus_tag="PKN51_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HNT85054.1"
                     /translation="MTRPSYDTTAAYPRDLAGYGRDAPHARWPGGARLAVQFVLNYEE
                     GGENCVLHGDAASEQFLSEMFNPAAYPDRHLSMEGIYEYGARAGVWRILREFDARGWP
                     LTVFGVASALARQPEVLRAFQARGDEIACHGLKWIHYQNVDEATERAHMAEALALIER
                     LSGTRPLGWYTGRDSPNTRRLVVDDGRLLYDSDYYGDDLPFWMKVRRSDGAVVPHLVV
                     PYTLDCNDMRFALPQGYSHAEPFFQYMKDSFDALYAEGDPAGLDRPKMLSIGMHCRLL
                     GRPGRITALQRFLDHVARHPDVWVCRRIDIARHWIATHLPQD"
     gene            complement(6611..7309)
                     /locus_tag="PKN51_07875"
     CDS             complement(6611..7309)
                     /locus_tag="PKN51_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT85055.1"
                     /translation="METLSSTDAIVQALTHAIVDHRLQPGSKLGEQALADHFGVSRTL
                     VRQALFQLSQKRLVTLAPARGAFVASPSADEARQVFAVRRMLELELTRAFVRQASPAD
                     IDALRAHVAAEQAAVAGGDVAGRTELLGDFHVRMAELLGNAVLAEVLRDLLARCAIAI
                     LMYQSSHAAHDSAAEHAALVGCFAQGNVTQAVKLMRAHLDHVEAGLQLDQPAPTNDIR
                     TALMPTSQAPAAIV"
     gene            7383..7736
                     /gene="uraH"
                     /locus_tag="PKN51_07880"
     CDS             7383..7736
                     /gene="uraH"
                     /locus_tag="PKN51_07880"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HNT85056.1"
                     /translation="MGLSTHVLDTMHGTPAAGMAVALYETHGEVATLLRRFTLNADGR
                     NPDGPLFDNASMKVGTYRLSFDVAGYFRARGVALPQPPFLDQVHLDFGIAQVDQHYHV
                     PLLCSPWSYSTYRGS"
     gene            7819..8634
                     /gene="xdhC"
                     /locus_tag="PKN51_07885"
     CDS             7819..8634
                     /gene="xdhC"
                     /locus_tag="PKN51_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HNT85057.1"
                     /translation="MRELAELLARLPQAPAALVTVVRSAGSVPRELGAWLAVFGDGVV
                     LGTIGGGHLELEAIERARAALAAGPFEPEERRCALGPSLGQCCGGVVWLRQERIEAAH
                     IEALRTRLMPATRPLALFGGGHVGRAIVRLLEELPFAVRWVDSRDEVFPAVLPLNVQA
                     EHSDPVQAAVAELPAGSAVLVMSFSHAEDLEVIAACLARQRERGDLSHIGLIGSRTKW
                     ARFGHRLAERGFSQAELARVSCPIGLPEIRGKQPAVVALAVVAELLALGLAPG"
     gene            complement(8696..8854)
                     /locus_tag="PKN51_07890"
     CDS             complement(8696..8854)
                     /locus_tag="PKN51_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85058.1"
                     /translation="MGNKIVTEAMRKATPPLPGAPRVKQADGQRRSLERGVHTRSKKN
                     PGKRPHQG"
     gene            9118..10326
                     /locus_tag="PKN51_07895"
     CDS             9118..10326
                     /locus_tag="PKN51_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNT85059.1"
                     /translation="MEAYLLDWANLLLRWLHVITAIAWIGSSFYFVFLDSSLLAPTAE
                     DLKVKGVDGAIWSVHGGGFYHAQKYMVAPKGGIDSHLHWFYWESYWTWISGFALFVVL
                     YLWNAGIFLVDKQVFDWSPLAAGIGALVFLAAFWFIYDAICRIFGQRANGDRIVGALV
                     LAVVVCSSWIAFQLFSGRAAFLMVGASLATAMSANVFFWIIPGQRKVVAAMTSGQPYN
                     PADYAIYGKRGKQRSVHNTYFTLPVLVAMLSNHYSFIYSHPQRWAILLVLMLAGALIR
                     LFFVQRHGYKLGRVGNPWPYAAAGVVLLLALIAWLKPAPAVPAAALPAKVSYAQIQPI
                     LAQRCYVCHGEAVQQKNLRVDSEALVSANAQLIYQQVVASKLMPLANSTQMTEDERAL
                     VGRWFQGQGK"
     gene            complement(10376..10624)
                     /locus_tag="PKN51_07900"
     CDS             complement(10376..10624)
                     /locus_tag="PKN51_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNT85060.1"
                     /translation="MSIIVTLVVGFIVGLIARAIMPGNQSMGLIMTTILGIVGSLVAG
                     YGGAALGLYAAGTPAGWIASVVGAIIVLWIYGMVSKKG"
     gene            complement(10854..12995)
                     /locus_tag="PKN51_07905"
     CDS             complement(10854..12995)
                     /locus_tag="PKN51_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT85061.1"
                     /translation="MIQLSNVSLRRGARLLLDGASVTLHPGEKVGLVGRNGAGKSSLF
                     ALLSGALHEDSGDCAMPAGWRLAQVAQQLPETEDSTTDFVLQGDERLSELRRQLSRAE
                     QTQDGMAIAQAHSDLHDAGAHDARARAEALILGLGFQPHELARPVNRFSGGWRMRLQL
                     ARALMAPSDLLLLDEPTNHLDLDALVWLEGWLARYAGTLIVISHDREFLDAVTRVTLH
                     IEGGRLTRYGGNYSAFEDLRAEQLSQQQAAWARQQDKIAHLQKFIDRFKAKATKARQA
                     QSRVKALERMERIAPVLAEAEFQLEFKAPLSLPNPMLAIRDAAFGYRADAIKKEAAGA
                     DPASPSGASGTESAAPPAYATSATVLAEQAARGELPPGLRPGDRVILRGVSRTVLAGQ
                     RIGILGANGQGKSTLVKTIARELAPLAGSVTEGKGLAIGYFAQQELDVLHTDSTPLEH
                     MVRLARQSGREASEEAREQQLRNYLGTFNFPGDMVNQPVGQFSGGEKARLVLAMIVWQ
                     RPNLLLLDEPTNHLDLATREALAVALNEFEGTLMLVSHDRALLRSVCDEFWLVARGSV
                     QPFDGDLEDYQRHLLDEARRLRERVVACAPETIADGASQESARAPKPAEPPLNPAEQR
                     RAQAQRRQQLAEQTRPLRRALAQAEQRMEQLGAERSALEQRLSSPLPAGDIADAGRRL
                     KALAEELQTQEDEWLRLSGEIEAIEQGAAAT"
     gene            complement(12992..13864)
                     /gene="prmB"
                     /locus_tag="PKN51_07910"
     CDS             complement(12992..13864)
                     /gene="prmB"
                     /locus_tag="PKN51_07910"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNT85062.1"
                     /translation="MTTLTQLITDTERQLSAAGVAFGHGTTNARDEAAWLVLHALGLP
                     LDTDLSGSEGNQPVSPAGQAQAAMLLKARIDTRQPLAYLTGQAWLQGVPFHVDARAIV
                     PRSLIAELIADGGIDPWLSERTRRVLDLCTGNGSLAVLAALAWPEVQVDAADLSRDAL
                     AVARLNVERHGLQQRIRLIESDGLAACPGPYDLIVCNPPYVNAASMAALPPEFRAEPA
                     LALAGGADGMDFIRPLLRQAPAHLNAGGVLVLEIGHERAHFDAAFPQLQPVWLDTSAG
                     DAQVLLLTREALAP"
     gene            complement(13917..15104)
                     /gene="dapE"
                     /locus_tag="PKN51_07915"
     CDS             complement(13917..15104)
                     /gene="dapE"
                     /locus_tag="PKN51_07915"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNT85063.1"
                     /translation="MTTTRRLAEALIARPSVTPADGGCIDLIAERLAPLGFEPEFIDR
                     GPPEWRVRNLWSKRVPAQYQQAPQAIETIVFAGHVDVVPTGPVEAWTSPPFTPTERAG
                     RLYGRGASDMKGAIAAFVVALEEFLAARPEAPLALALLLTSDEEGRALDGTRVVCEWL
                     AARGERLHACIVGEPTAQRHSGDMVKNGRRGSLSGRLVVRGVQGHVAYPHLARNPIHQ
                     LAPALAELAATAWDAGNAHFQPTGWQVSNLHAGTGAGNVIPGQAVVDFNFRYGTASTP
                     ASLQARLQAVLQRHGLDHTLEWTLGAEPFLTPPGSLLDAVQAAIAAETGLQPELSTTG
                     GTSDGRFLARICPQVIELGSPNASIHQIDEHIELAELERLKNIYRRVLEQLAAQARVA
                     QLR"
     gene            complement(15288..16559)
                     /locus_tag="PKN51_07920"
     CDS             complement(15288..16559)
                     /locus_tag="PKN51_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNT85064.1"
                     /translation="MPKPVASTTMMDRILHLMAEKKASDVYLSAHAPATIRINGVSMP
                     INNQVLPPEATLNLLMDVVPPNRIEELKETGELNMAIPIYGVGNFRLSAMRQRGTYAA
                     VIRFISPEIPDLDSLNLPSILKHLVMEKRGLVLMVGATGNGKSTTLAAMLDHRNRNAT
                     GHILTIEDPIEYVFTNHKSLINQREVGTDTAALQIGLKNALRQAPDVIMIGEIRDRET
                     MTAAIAYAQSGHLCLATLHANNSYQALNRVLSFYPVEVRPTLLGDLSAALKSIVSQRL
                     VRTTEGSRVPAVEVLLNTKLVADLIEKSDFSGVKDAMDNAIAEGSQTFEQDLARLILE
                     NRIDRKEGLANADSPTNLMWRLQNQTVRHLARKPEEEERPSDEPSFTDITLDVRPSGF
                     GKSGFSRSGYRDSGFQDSKVGSFSDSHIGDA"
     gene            16910..17461
                     /locus_tag="PKN51_07925"
     CDS             16910..17461
                     /locus_tag="PKN51_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85065.1"
                     /translation="MIYAKSESGLQAVKDRHAVDLTRAQRSVLILIDGKRSVREVLEA
                     TATLGVTATDVELLVQRGLVVVASGGMSLNGSAGGASLNGSSGGAGRVTATGDGAASG
                     SGDPAAPDLTDDERARRYRVAYPMATQLTAKLGLKGFRLNLAVEEAAGFEGLVDLLPR
                     LRSALGSEGVRPLESVLIGDPLQ"
     gene            17687..>18158
                     /locus_tag="PKN51_07930"
     CDS             17687..>18158
                     /locus_tag="PKN51_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT85066.1"
                     /translation="MRLNSIKLSGFKSFAEPTNFMLPGQLVGVVGPNGCGKSNIMDAV
                     RWVLGESKASELRGESMQDVIFNGTTTRKPASRSSVELVFDNSDHRAGGQWSQFTEIS
                     VKRVLTRDGTSSYYINNQPVRRRDVQDVFLGTGLGPRAYAIIGQGTISRIIESRP"
BASE COUNT         2649 a         5891 c         6562 g         3056 t
ORIGIN      
        1 cagctgcgcg gcgcgtggcc gcaggcatgg tggagccatg tcaaggccac gccaccaagc
       61 agatggcccc ttggcggctc accctgcggg aagccagaaa aagggcttgc ggcggcgttg
      121 cgcctcttgc caaggccgcc agcatgggct gcgagccgcg ccttgccgca agccttgttc
      181 tggcttctgt aagtgcctga cagcgcgaga cactgcaccg gtaccccgaa aacaggagcg
      241 gccggcgcag gcccagcctg cgccagccgc cattctgatg cagacattca gggcgtcagc
      301 gcaccagctc gggcagcttg tcggtgcggc tggcccaggc ttcgtggtcg ggcagcggat
      361 ccttgcgttt ggtcagcgtc cggtagttgg gcgaacgggc caactcggcg ttgatctgcg
      421 tcatgtgcaa ctggtccttg ggcaggtctt cctcggcaaa gatggcgttg accgggcact
      481 cgggaatgca gacggcgcag tcgatgcact catcggggtc gatggtgaga aagttggggc
      541 cttccttgaa gcagtcgacg gggcaaacgt ccacgcaatc ggtgtatttg cagttgatgc
      601 aggcttcggt gacgacgtgg gtcatggtca gaactcgtgg gcaaagcgtg aaagcaggca
      661 ggcgccggtc tgaatgctca gcgccggcac ctgcagccat gcgcgaattt taagaggggg
      721 catcggcggc cggtcgccgt gcggttttgg acggtggtct gactgccggt cacttgatgc
      781 aagtcaattg gcgaacggtc gaaattgccc ggcgggctgc ccgcgcactg tagccaccct
      841 gcggcccgga tccaaccatt cgtttgttct ggccatatgc gccagcggca taagcgcgcc
      901 aggtggttgc cgaaccgtcg gcacacgaac aaaaccggcc accgcgggcc ggttcgtggg
      961 ttggggcagt gctcagccag cgagcatcaa tgcgcttcag cctgtcgggc ggcgcgaatg
     1021 gaagcttcgg tgtcggcgcg ggcgccgttg aagaacagat tcagcagcac ggcggcgatg
     1081 gaggccagca cgatgcccga ctcgatcagc gggtggatgg cgtgcggcat ccactgcagg
     1141 tacctgggcg ccaccagcgg gatcatgccg acaccgatgg agatcgccac gatcagcgcg
     1201 ttgttgcggt tgtgggtgta gtccacccca cccaggatgc ggatgccggt ggccgccacc
     1261 atgccgaaca tcacgatgcc ggcgccgccc agcaccacgg tgggcagcga ctccaccagc
     1321 gccgccatct tgggcagcag gcccagcacg atcaggatga ggccgccggc cacgcagacg
     1381 aagcggctct tgacgccggt gaccgccacc aggccgacgt tctgcgagaa gctggtgtag
     1441 gggaaggtgt tgaagatgcc gccgatcagc gtgcccagac cgtcggtgcg caggccgcgc
     1501 gagagcatcg gctggtcgat cctgcggtcg gtcatctcgc ccagcgcgag gaacatgccg
     1561 gtcgattcga tcatcaccac gatcatgatc agcgtcatgg tcaggatcat caccgggtcg
     1621 aaaatcggcg tgccgaagtg aaacggtgtc acgacgtcga accaggcggc cttgccgacc
     1681 ttgtcgaagt tcatcatgcc ggtggcggtg gccacgatgc cgccgatgac gatgcccagc
     1741 agcaccgaga tgttggcgat gaagcccttg gcgaacttgg cgatcagcag gatcgaggcc
     1801 agcacgatgg ccgacaccgc cacccccgac agcttggcgt agttggggtt gggcacggtc
     1861 ggcgccagcg tcaggccctg cggcaccggc agcaccccgg gctggccggt ggcgccggcc
     1921 gaacgcatgg tgctgagcca ttcggtgtgc tcgggcgaga gggtcagcgg tgcggtcggg
     1981 ccgaccgggt tgccgaagat ccagttgatg cccacgcgca tcaggttgat gccgatcacc
     2041 gcgatgatgg tgccggtgac caccggcgga aagaagcgca gcatgcggct gaccaccggc
     2101 gcgatcagga tcgagatgat gccggcgccg atgatggcgc cgaacagcat gcgcgccccg
     2161 tcggtgccgg gattgccgtt ggcgatcgcc accatcgggc cgacggcggc gaaggtcacg
     2221 cccatcatca ccggcaggcg gatgccgaac cactgcgtgg cgcccagcgc ctggatcagg
     2281 gtggcgatgc cgcagcagaa caggtcggcc gagatcagga aggccacctc ctgcggcccc
     2341 agcttgagcg cgcggccgac gatcagcggc acggcgacgg cgcccgcgta catcaccagc
     2401 acatgttgca gcccgagggc cgtgagtttg ccggtgggca gacgttcatc gacgggatgg
     2461 acggcggtgg tgctcatgtt gtctcctgaa gtttgtcgtc gttgagcaag aaaagggggc
     2521 gcgggcggtc agttgtgcag caggtcggcc agcgcgcgtg cgatgacctt ggtggcgcgg
     2581 cgcagatcct gcaggtcgac gcgctcgtcg gcgcgcttgg cgtggctttc cagcacgctg
     2641 cgcgggccgg cgccgtagat cacgccgggg atgccgcgct cgacatacag gcgcacgtcg
     2701 gtgtagagcg gtgtgccgac ggccggcggg cgcgcgccaa acaccgcctc gccgtgcttc
     2761 tggatcgcct cgaccagcgg ccggttgccg ggcagcggcg tcatggcgtg ggccagcagg
     2821 atgcggcgca cgtcggtgcg cacggcgtcc tcgcccttca caccgcgcca gcggttgaag
     2881 tcgcgcgtgg cgccttcgat gaccttgcgg atgcgcgcct cgaccttggc tggcttttcc
     2941 tccgggatca tgcggcggtc gagcttgagc accaccttgc cggggatcac gttggtgttg
     3001 gtgccgccct cgatgcggcc gatgttgagg taggggtgcc ggatgcccgg caccttggag
     3061 ctgaccttct ggtaggcgct gttttcggca tagagcgcgt tcatgatgaa caccgccgcc
     3121 tgcagcgcgt ccactccggt gtgcggcacc gccgcgtgcg ccatccggcc ctgcaccgtc
     3181 acttccatct gcaggcagcc gttgtgggcg gtgacgactt cgtagctgaa gccggcggcg
     3241 atcatcaggt cgggccgggt caacccctgc gccagcagcc agccggggcc cagcaggccg
     3301 ccgaattcct cgtcgtaggt gaaatgcagc tcgacggtgc cgcgcgtggg cttggccacc
     3361 gcttccagcg cgcgcacggc gaagctgaag gtggaaaagt cgctcttgct caccgcggtg
     3421 gcgcggccga acatctggcc gccctcgatt tcggcgccat acgggtcatg cgtccagcct
     3481 tcgccgggcg gcaccacgtc gccgtgggcg ttgagcgcga tcgttcggcc acccttgccg
     3541 tagggacggc gcacgatcag gttggtgatg ctttgcatgc cgtagtcgcg cacttcctgc
     3601 tcgggcaccg gatggcgctc ggcctcgaag ccccaggcgg cgatcagctc ggccgtgcgt
     3661 tcggcatgcg gtgcgttgtt gcccggcggc gtgtcggtgg gcacgcgcac cagttgctgc
     3721 aggaagcgca cctgctcgtc gaagtgctgg tcgatccagg cgtcgagccg ggcgtaggtg
     3781 tcggtggcag tggtcatggt ggggtcttga atgaaaaatg gctctggcgc ccgatgggca
     3841 agcgccggca gctatgattg tgatagcgta tcgcgcgcca tgccgtccag caggtgctgc
     3901 atggcgtgca ccgccagcgc catgtcttcg gcgctggtgg attcgagtgg gttgtggctg
     3961 atgccggcgt tctcgccacg cacgaacagc atggcctgcg gcatgatttc gtgcagcttc
     4021 atggcgtcgt ggccggcgcc gctgggcatg cgaaacaccg gcaggcccag cgcctccacc
     4081 gcgtcggccc agcgctgctg cagcgccgcg tcgcagggtg cggcggcggc gcgcatggtt
     4141 tcttcgagct gcaggcgcac gccgcggcgc tcggcgatcg cctgggcctc ggccagcacg
     4201 tcgctcacca gggcgtcgcg ctgcgcatcg ctgggggcgc ggaggtccag gctgaagttg
     4261 cagcgccccg gcaccacgtt gatcgatcca ttgggcacgg tcagcatgcc gatggtgccg
     4321 accgagtcgc cgtcctgcgc ggcgcggcgc tccagataca gcgccagctc ggccgcagcg
     4381 caggcggcgt cgcgccggcg gtccatgggc gtggtgccgg cgtggctggc ctggccgatg
     4441 taggcaccct ggtagcgcac gctgccgttg atggaagtca ccacgcccag cggcaggttc
     4501 agttcgttca gcaccgggcc ctgctcgatg tgcacctcga tgaagcccag gtagtcggcg
     4561 gcgtggcgct tgagcttggg gatgtcgtcc acgcacaggc cggcgtgctg catggcctcg
     4621 cgcatgctga tgccgtcggc gtcgcgctgg tccagccatt cggggcgaaa gtcaccgatc
     4681 agcgcgcccg aacccaggaa ggtggccttg tagcgctggc cttcttcctc ggcaaagccg
     4741 accacttcga ggtcgaacgg caggcggcgc ccggtgcgcg ccagctcctg cacgcaggcc
     4801 aggggcacga agatgcccag gcgcccgtcg tacttgccgc cgttgcgcac cgtgtcgtag
     4861 tggctgccgg tgagcagggt cttggggttg cgcgcgcccg ggtcggcctt gtagcggccc
     4921 accacgttgc cgacggcgtc gatgtgcacg tcgtcgaacc cggcatcgcg catgttgcgg
     4981 ctgatgcgct gggcgcaggc gcggtgcgcg tcggtcaggt aggtgacggt cagctggccg
     5041 gcctcggcgc agccggggtc gctgtgctgg gccagcttct cgtgccagtc ccacacctgt
     5101 tcgcccagcg tgggcggagc gccgaacttg tcggccaggc ggatctcgac gatgcggtgg
     5161 atctggcgca gcgcctcggc gcgctcgaag tcggggtggt ggtgcaggcg gcgttcgaag
     5221 gtgtcgagga tctgctgctt ggccaggccc gtgccgcgcg ggccgcgcac gcacagcacg
     5281 aacggaaagc cgaagcgcgc ctgatagtcg gcgttgagct ggcggatgcg cgccagctcc
     5341 tcgggcgtgc aggcggtgag gccggcgcgc tgctgctcgc cggtcgattc gctggtcagc
     5401 gtgccggctt ccatggcctt gccggccagt tccgggtggg cgcggatcag cgccagctgg
     5461 gcgtccacgc tggcgcgcgt cagcgcgccg gccatggcct gcttgagctg cgccagcgat
     5521 tggaacggtc gcgcctgcag cgcctgggcg gcgatccagg gcgtgtgctc gtacaggccg
     5581 tcgagcatgc gctcggcttc ggccagcggt gccgcgttga gttgatccag cgtgtagggc
     5641 atgggcggtc agtcctgagg caggtgggtg gcgatccagt ggcgcgcgat gtcgatgcgg
     5701 cggcacaccc acacgtccgg atgccgtgcc acatggtcca gaaagcgctg cagcgcggtg
     5761 atgcgcccgg gacgcccgag cagacggcag tgcatgccga tgctcagcat cttggggcgg
     5821 tccagaccgg ccgggtcgcc ctccgcgtac agggcgtcga agctgtcctt catgtactga
     5881 aagaacggtt cggcgtggct gtagccttgc ggcagggcaa agcgcatgtc gttgcagtcc
     5941 agcgtgtagg gcaccaccag gtgcggcacc accgcgccgt cgctgcggcg caccttcatc
     6001 cagaacggca ggtcgtcgcc gtagtagtcg ctgtcgtaca gcagccggcc gtcgtcgacc
     6061 accaggcggc gcgtgttggg gctgtcgcgg ccggtgtacc agcccagcgg gcgcgtgccc
     6121 gacaggcgtt cgatgagcgc cagcgcctcg gccatgtggg cgcgctcggt ggcttcgtcg
     6181 acgttctggt agtggatcca cttcagaccg tgacaggcga tctcgtcgcc gcgcgcctga
     6241 aaggcccgca gcacctcggg ctggcgcgcc agggcgctgg ccacgccgaa caccgtcagc
     6301 ggccagccgc gggcgtcgaa ctcgcgcagg atgcgccaca cgccggcgcg cgcgccgtac
     6361 tcgtagatgc cctccatgct cagatggcgg tcggggtagg cggccgggtt gaacatctcg
     6421 gacagaaact gctcgctggc ggcgtcgccg tgcagcacgc agttttcgcc gccttcttcg
     6481 tagttcagga cgaattgcac cgcaaggcgc gccccgcccg gccagcgggc gtgcggggcg
     6541 tcgcggccgt agccggccag gtcgcgtggg taggcggcgg tggtgtcgta ggagggtcgt
     6601 gtcatgctat tcaaacaata gctgctggcg cttgactggt gggcatcaaa gctgttctga
     6661 tgtcgtttgt gggggccggc tggtcgagct gcaggccggc ttccacatgg tccaggtggg
     6721 cgcgcatcag tttcaccgcc tgcgtcacgt tgccctgggc aaaacagccc accagcgccg
     6781 cgtgctcggc ggccgaatcg tgcgcggcgt ggctgctctg gtacatcagg atggcgatgg
     6841 cgcagcgcgc cagcaggtcg cgcagcacct cggccagcac ggcgttgccc agcagttcgg
     6901 ccatgcgcac gtggaagtcg cccagcaact cggtgcggcc ggccacgtcg ccaccggcca
     6961 ccgccgcctg ctcggccgcc acgtgcgcgc gcagggcgtc gatgtcggcc gggctggcct
     7021 ggcgcacgaa ggcgcgcgtg agctccagct ccagcatgcg gcgcaccgcg aacacctgcc
     7081 gcgcctcgtc ggccgacggg ctggcgacga aagcgccgcg cgccggcgcc agcgtcacca
     7141 ggcgcttctg ggacagctgg aacagggcct gccgcaccag cgtgcgcgag acgccaaaat
     7201 ggtccgccag cgcctgctca cccagcttgc tgccgggttg cagtcggtga tcgacgatgg
     7261 cgtgggtcag cgcctgaacg atcgcatcgg tgctggacag ggtttccatg gcccgccatg
     7321 ataggcataa ttccaaaaat tgtatacaac cctgtgcaca atgcattcaa ggagtggttg
     7381 atatgggact cagcacgcat gttctcgaca ccatgcacgg cacgcccgcg gccggcatgg
     7441 cggtggcgct gtatgaaacg cacggcgaag tggcgacgct gctcaggcgc ttcacgctca
     7501 acgccgacgg ccgcaacccg gacggcccgc tgttcgacaa cgccagcatg aaggtcggca
     7561 cctatcgtct gagcttcgac gttgccggct acttccgcgc gcgtggcgtg gcgctgccgc
     7621 agccgccgtt tctggaccag gtgcacctgg acttcggcat cgcccaggtc gatcagcact
     7681 accacgtgcc actgctgtgc agcccctgga gctattcgac ctaccgcggc tcgtgagcgc
     7741 gcccgctcag gccccgggtg gccggcatga ccgggcggcc ggcccggccg cggcccccca
     7801 tgggcgcggc acaccatgat gcgcgagctg gccgagctgc tggcgcgcct gccacaggcc
     7861 cccgcggcgt tggtgacggt ggtgcgcagc gctggctcgg tgccgcgcga gctcggcgcc
     7921 tggctggccg tgttcggcga tggcgtggtg ctgggcacca tcggcggcgg gcacctggag
     7981 ctggaggcca tcgagcgcgc ccgggcggcg ctggccgccg gcccgttcga gcccgaggag
     8041 cggcgctgcg cgctcggccc cagcctgggc caatgctgtg gcggcgtggt gtggctgcgc
     8101 caggagcgca tcgaggcggc gcacatcgag gccctgcgca cccggctgat gcccgccacc
     8161 cgaccgctgg cgctgttcgg cggcggccat gtggggcgcg ccatcgtgcg cctgctggag
     8221 gagctgccgt ttgccgtgcg ctgggtggac agccgcgacg aggtgtttcc ggccgtgctg
     8281 ccgctgaatg tgcaggccga gcattccgac ccggttcagg cggcggtggc cgagctgccg
     8341 gcgggcagcg cggtgctggt gatgagcttc agccatgccg aggacctgga ggtcatcgcc
     8401 gcctgtctgg cgcgccagcg cgagcggggt gacctgagcc acatcggcct gatcggcagc
     8461 cgcaccaaat gggcccgctt tggccatcgc ctggccgaac gcggcttcag ccaggccgaa
     8521 ctggcgcgcg tgagctgccc gatcggtctg ccggagatcc ggggcaagca gccggcggtg
     8581 gtggcgctgg cggtggtggc ggaactgctg gcgctggggc tggcgccggg ctgaagccaa
     8641 aaaaatggcg cccgctgggc gccatggctg agggcgggcg aaggccgctg gacgctcagc
     8701 cctgatgcgg gcgcttgccc ggattcttct tgctgcgcgt gtgcacgccg cgctccaggc
     8761 tgcggcgctg gccatcggcc tgcttgacgc ggggggcgcc gggcagcggc ggggtggcct
     8821 tgcgcatggc ctcggtgacg atcttgttgc ccatggtgtg atccttgtga agtggtgtgt
     8881 ggacgaaaac cctatattgt cgccgattcg cagcccatcg tggcaagctc ggcacaaggg
     8941 aaaacctggg cgccaggccc accgaactgc atacccaatt gtatacaatc cgaccgagat
     9001 cgcagcggtg cccgagagtc gtccatgagg cagtgcgtga cgccccccga tagcgcgatc
     9061 tcctgaaagc ggcttgcagc gcccgcggtg gcaagcccga tgaagggaca ctcacgcatg
     9121 gaagcatatc ttctcgactg ggccaacctg ctgctgcgct ggctgcacgt catcaccgcc
     9181 atcgcctgga tcggctcgtc cttctatttc gtgtttctcg acagcagcct gctggcgccc
     9241 accgccgaag acctgaaggt caagggtgtc gacggcgcca tctggtcggt gcacggcggg
     9301 ggcttctacc acgcgcagaa gtacatggtg gcgcccaagg gcggcatcga cagccacctg
     9361 cactggttct attgggaaag ctactggacc tggatctccg gctttgcgct gttcgtggtg
     9421 ctctacctgt ggaacgccgg catcttcctg gtcgacaaac aggtgttcga ctggtcgccg
     9481 ctggccgccg gcattggcgc gctggtgttc ctggcggcgt tctggttcat ctacgacgcc
     9541 atctgccgca tcttcggtca gcgtgcgaac ggcgaccgca tcgtcggcgc gctggtgctg
     9601 gcggtggtgg tgtgctcgtc gtggattgcg ttccagctgt tttccggccg tgcggcgttt
     9661 ctgatggtgg gcgcgtcgct ggccaccgcc atgagcgcca acgtgttctt ctggatcatc
     9721 cccggccagc gcaaggtggt ggcggccatg accagcggcc agccctacaa cccggccgac
     9781 tacgccatct acggcaagcg cggcaagcag cgcagcgtgc acaacaccta cttcacgctg
     9841 ccggtgctgg tggccatgct cagcaaccac tacagcttca tctacagcca cccgcagcgc
     9901 tgggcgattc tgctggtgct gatgctggcc ggcgcgctga ttcgcctgtt cttcgtgcag
     9961 cgccacggct acaagctggg ccgcgtgggc aacccctggc cctatgcggc cgccggcgtg
    10021 gtgctgctgc tggcgctgat cgcctggctc aagccggccc cggcggtgcc cgcggcggcc
    10081 ctgccggcca aggtgagcta cgcgcagatc cagccgatcc tggcgcagcg ctgctacgtc
    10141 tgccacggcg aggccgtgca gcagaagaac ctgcgcgtgg acagcgaggc gctggtcagc
    10201 gccaacgccc agctgatcta ccagcaggtg gtggccagca agctgatgcc gctggccaat
    10261 tcgacccaga tgaccgagga cgagcgcgcc ttggtgggcc gctggttcca aggtcagggc
    10321 aagtgagccc gacgcgccgc cggcgtgggt tgccggtggc gggcagacgc gacgctcagc
    10381 ccttcttgct caccatgccg tagatccaca gcacgatgat ggcgccgacc accgaggcga
    10441 tccagccagc cggcgtgccg gctgcgtaca ggcccagggc ggcgccgccg tagccggcca
    10501 ccagcgagcc gacgatgccc aggatggtgg tcatgatcag gcccatgctc tggttgcccg
    10561 gcatgatggc gcgcgcgatc aggccgacga tgaagccgac gacgagggtg acgatgatgc
    10621 tcatgttgga tgccttccct ggtggatgaa cgctggacgg cctgcactgt accgcaccca
    10681 tcggtcgtcc acacgggcgc gcggggcgcg cggcggccgc cacggcgcgc cgtcggtggc
    10741 gtgacggttc atgccaactc gcatgaccaa cgaccaacga ccaatgacgg atgacctcgc
    10801 tgcgccgcga ggtccaaggg gtcatgcgtc gcggtttttg aacgccaagc ccatcaggtt
    10861 gcggcggcgc cttgctcgat ggcttcgatc tcacccgaca ggcgcagcca ctcgtcttcc
    10921 tgcgtctgca gctcctcggc cagcgccttc agtcgccggc cggcgtcggc gatgtcgccg
    10981 gcgggcaggg ggctggacag gcgttgctcg agcgcgctgc gctcggcgcc gagctgttcc
    11041 atgcgctgct cggcctgcgc cagtgcgcgc cgcagcggcc gggtctgctc cgccagctgc
    11101 tggcgccgct gcgcctgggc gcgccgctgc tcggccggat tgagtggcgg ttcagcaggt
    11161 tttggggccc tggcgctttc ctggcttgcg ccgtcagcta tggtttcagg agcgcacgcg
    11221 acgacccgct cgcgcaaccg gcgcgcttca tccagcaggt ggcgctggta gtcttccagg
    11281 tcgccgtcga acggctgcac gctgccgcgc gccaccagcc agaactcgtc gcacacgctg
    11341 cgcagcaggg cgcggtcgtg gctgaccagc atcagcgtgc cttcgaattc gttgagcgcc
    11401 accgccagcg cctcgcgcgt ggccaggtcc aggtggttgg tgggctcgtc cagcagcagc
    11461 aggttggggc gctgccagac gatcatggcc agcaccaggc gcgccttttc gccgccgctg
    11521 aactggccta ccggctggtt gaccatgtcg cccgggaagt tgaaggtgcc caggtagttg
    11581 cgcagttgct gctcgcgcgc ctcttcgctg gcttcgcgcc cgctctggcg cgccagccgc
    11641 accatgtgct cgagcggcgt gctgtcggtg tgcagcacgt cgagttcctg ctgggcgaag
    11701 tagccgatgg ccaggccctt gccctcggtc acgctgccgg ccaggggcgc cagctcgcgc
    11761 gcgatggtct tgaccagggt ggatttgcct tgcccgttgg cacccaggat gccgatgcgc
    11821 tggccggcca gcacggtgcg gctgacgccg cgcaggatca cgcggtcgcc gggccgcagg
    11881 ccgggcggca gctcgccgcg cgcggcctgt tcggccagca cggtggcgct ggtggcgtag
    11941 gccgggggtg ccgcactttc agtgccggaa gcgccgcttg gacttgccgg gtctgcgcca
    12001 gcagcttctt ttttgatagc gtcggcacgg tagccgaaag ccgcgtcgcg gatcgccagc
    12061 atggggttgg gcaggctgag cggggccttg aactcgagct ggaactcggc ctcggccagc
    12121 accggggcga tgcgttccat gcgctccagc gccttgacgc ggctttgcgc ctggcgcgcc
    12181 ttggtggcct tggccttgaa gcggtcgatg aacttctgca ggtgggcgat cttgtcctgc
    12241 tggcgcgccc aggccgcctg ctgctggctg agctgctcgg cgcgcaggtc ttcgaaggcg
    12301 ctgtagttgc cgccgtagcg ggtcaggcgc ccgccctcga tgtgcagcgt gacgcgggtg
    12361 acggcgtcga ggaactcgcg gtcgtggctg atgacgatca gcgtgccggc atagcgcgcc
    12421 agccagcctt ccagccacac cagcgcgtcc aggtcgaggt ggttggtggg ttcgtcgagc
    12481 agcagcaggt cgctgggcgc catcagcgcg cgcgccagtt gcagtcgcat gcgccagccg
    12541 cccgaaaagc ggttgaccgg ccgcgccagc tcgtgcggct gaaagcccag gcccaggatc
    12601 agcgcctcgg cgcgcgcgcg ggcgtcgtgc gcgccggcgt cgtgcaggtc gctgtgggcc
    12661 tgggcgatcg ccatgccgtc ctgggtttgc tcggctctgg acagctggcg gcgcagctcg
    12721 gacaggcgct cgtcgccttg cagcacgaaa tcggtggtgc tgtcttcggt ttcgggcaac
    12781 tgctgtgcca cctgggccag gcgccagccg gcgggcatgg cgcagtcgcc gctgtcttcg
    12841 tgcagcgcgc cgctcagcag cgcgaacagg ctggacttgc cggcgccgtt gcggccgacc
    12901 aggccgacct tttcgcccgg gtgcagggtg acgctggcgc cgtccagcag caaccgggcg
    12961 ccgcggcgca ggctgacgtt gctgagctgg atcatggcgc cagcgcctcg cgcgtgagca
    13021 gcagcacctg cgcgtcgccg gcgctggtgt ccagccacac cggttgcagc tgcgggaatg
    13081 cggcgtcgaa gtgcgcgcgt tcgtggccga tctccagcac cagcacgccg ccggcgttca
    13141 ggtgcgccgg cgcctggcgc agcagcgggc ggatgaagtc catgccatcg gcgccgccgg
    13201 ccagcgccag cgcgggctcg gcgcgaaact cgggcggcag cgcggccatg ctggcggcgt
    13261 tgacgtaggg cgggttgcac acgatcaggt cgtaggggcc ggggcaggcg gccaggccgt
    13321 cgctttcgat cagccggatg cgctgctgca ggccgtggcg ctcgacgttc aggcgcgcca
    13381 ccgccagcgc atcgcggctc aggtcggccg catccacctg cacctcgggc caggccaggg
    13441 cggccagcac ggccaggctg ccgttgccgg tgcacaggtc gagcacgcgg cgtgtgcgtt
    13501 cggacagcca ggggtcgatg ccgccgtcgg cgatcagttc ggcgatcagg ctgcgcggca
    13561 cgatggcgcg cgcgtccaca tgaaacggca cgccttgcag ccaggcctgc ccggtcaggt
    13621 aggccagcgg ctggcgggtg tcgatgcgcg ctttcaaaag catagctgct tgcgcttgac
    13681 cggcgggcga cacgggctga tttccttcgg aaccgctgag gtcggtgtcg agcggcaggc
    13741 cgagcgcgtg cagcaccagc caggcggctt cgtcgcgggc gttggtggtg ccgtggccaa
    13801 aggccacgcc cgccgcgctg agctggcgtt cggtgtcggt gatgagttgg gtgagtgtgg
    13861 tcatgcggaa aacggcgccg gcgggtgcgt ggccggcgca ccgctcgggg tgaaggtcaa
    13921 cgcagctgcg ccacccgggc ctgggcggcc agctgctcca gcacgcggcg gtagatgttc
    13981 ttcagccgct ccagctcggc cagctcgatg tgttcgtcga tctggtggat gctggcgttg
    14041 ggcgatccca gctcgatcac ctgcgggcag atgcgggcca gaaagcgccc gtcgctggtg
    14101 ccgccagtgg tggacaactc gggctgcagg ccggtttcgg cggcgatggc ggcctgcacc
    14161 gcatccagca ggctgcccgg cggggtgagg aagggctcgg cccccagcgt ccactcgagc
    14221 gtgtggtcga gcccgtggcg ttgcagcacc gcctgcagcc gcgcctgcag gctggccggc
    14281 gtggaggcgg tgccgtagcg gaagttgaag tccaccaccg cctggcccgg gattacgttg
    14341 ccggcgccgg tgccggcgtg caggttgctg acctgccagc cggtgggctg aaagtgggcg
    14401 ttgccggcgt cccaggcggt ggcggccagt tcggccaggg cgggcgccag ctggtggatc
    14461 gggttgcgcg ccaagtgcgg ataggccacg tggccctgca cgccgcgcac caccagccgg
    14521 ccgctgagcg agccgcggcg gccgttcttg accatgtcgc cgctgtggcg ctgggcggtc
    14581 ggctcgccga cgatgcaggc gtgcagccgc tcgccgcgcg cggccagcca ctcgcacacc
    14641 acgcgggtgc catccagggc gcggccttct tcgtcgctgg tcagcagcag ggcgagggcc
    14701 agcggcgcct cgggccgggc ggcgaggaac tcctcgagcg ccaccacgaa ggcggcgatg
    14761 gcgcccttca tgtcgctggc gccacgcccg tacagccggc ccgcgcgctc ggtgggggtg
    14821 aagggcgggc tggtccaggc ttcgaccggg ccggtcggca ccacgtccac atggccggca
    14881 aatactatcg tttcaatagc ttgcggcgct tgttggtatt gcgctggaac ccgtttggac
    14941 cacagattgc ggacgcgcca ttcgggcggc ccgcggtcga tgaattcggg ctcgaacccg
    15001 agcggcgcca ggcgttcggc gatcaggtcg atgcagccgc cgtcggccgg cgtgacggag
    15061 gggcgggcga tcagggcttc ggccaggcgg cgcgtggtgg tcatggaggg gcgaggctgg
    15121 ggcgcggcgg tgagcaggcc cgcattcttt catgccccgg cgcgcgcggc ggggatgcag
    15181 gccggcatcg gcgtgccgcc ggacgaccca tgacgccccg ccttgcccct gacccatggg
    15241 tcatcgaagc gccgcgaggt cattcgacat gcgtcaggga tggaccctta ggcatcgccg
    15301 atgtgtgaat cggaaaagct gccgaccttg gagtcctgga agcccgaatc gcggtagccg
    15361 ctgcggctga acccggactt gccgaagccg ctggggcgca cgtcgagcgt gatgtcggtg
    15421 aaggacggtt cgtcgctcgg gcgctcttct tcttcgggct tgcgcgccag gtggcggaca
    15481 gtctggttct gcagtcgcca catcaggttg gtgggcgagt cggcgttggc caggccttcc
    15541 ttgcggtcga tgcggttttc caggatcagg cgcgccagat cctgctcgaa ggtctgcgag
    15601 ccttcggcga tggcgttgtc catggcgtcc ttgacgccgg agaagtcgct cttctcgatc
    15661 aggtcggcca ccagcttggt gttgagcagc acctccaccg ccggcacacg gctgccttcg
    15721 gtggtgcgca ccagacgctg ggaaacgatc gatttgagtg ccgccgacag gtctcccagc
    15781 agggtggggc gcacctcgac cgggtagaaa ctgagcacgc ggttgagcgc ctggtaactg
    15841 ttgttggcgt gcagcgtggc caggcacagg tggcccgact gcgcgtaggc gatggctgcg
    15901 gtcatggtct cgcggtcgcg gatctcgccg atcatgatca cgtcgggcgc ctgccgcagg
    15961 gcgttcttca gcccgatctg cagcgccgcc gtgtcggtgc ccacctcgcg ctgattgatg
    16021 agcgacttgt ggttggtgaa gacgtattcg atcggatcct cgatggtcag gatgtgacca
    16081 gtggcgttgc ggttgcggtg gtccagcatg gccgccaggg tggtggactt gccgttgccg
    16141 gtggcgccca ccatcagcac caggccgcgc ttttccatca ccaggtgctt gaggatgctg
    16201 ggcaggttga gcgagtccag gtccgggatc tcggggctga tgaagcggat caccgccgcg
    16261 taggtgccgc gctggcgcat ggccgacaga cggaaattgc ccacgccgta aatcgggatc
    16321 gccatgttca gctccccggt ctccttcaac tcctcgatgc ggttgggcgg caccacatcc
    16381 atcagcaggt tcagcgtcgc ctcggggggc agcacctggt tgttgatcgg catgctgacg
    16441 ccgttgatcc ggatggtggc cggtgcgtgg gccgacaggt acacgtccga ggcctttttt
    16501 tcggccatca ggtgcaggat gcggtccatc atggtggtgg aagcaacagg cttgggcatg
    16561 gcagtgactc ggatgctgga cgatggcgcg ccggtcgacg cgggtaggtc catggcgggg
    16621 cctgccggct cgcgccctga accagcctca ataggcaaaa cggtttcatt gtaggagcgc
    16681 aacgaccatc gtgaggcccc gacatgccga gaatttcacg ctcggcaccc tgttcaccac
    16741 agaccaatcg acaccagacg ttgcgctggt gctagtgtct ctcgcacagg aacgatccag
    16801 gaatgcggca caccggacta ctggaaagtc ctaacaagga aagcgttgcc aagcaaggcg
    16861 tttccaattg taatgtaatc atttggattg atatcctgac tgtctgaaca tgatttacgc
    16921 caaatccgaa tctggcctgc aggcggtcaa ggatcgccat gcggtggacc tgacccgagc
    16981 gcagcgctcg gtgctgatat tgattgacgg caaacgcagc gtgcgcgaag tgctggaggc
    17041 cacggcgacg ctgggtgtga cggcgacaga cgtcgagctg ctggtgcaac gcgggctggt
    17101 ggtggtggcc agcggcggca tgtcgctcaa cggtagcgcg ggcggtgcct cgctgaacgg
    17161 gtcgtccggc ggagccgggc gcgtgacggc gaccggtgat ggcgccgctt ccgggtcggg
    17221 tgatcctgcg gcacccgatc tgacggacga cgaacgcgct cggcgctacc gtgtggccta
    17281 tcccatggcc acccagctga cggccaaact gggactcaag ggcttccggt tgaacctggc
    17341 ggtggaggaa gcagctggct ttgagggtct ggtggacctg ttgccgcgcc tgcgcagtgc
    17401 cctgggcagc gagggtgtgc gtcctctgga gagcgtgctg atcggtgatc cgctgcagtg
    17461 acccgggaga tgcggcatcg tgggttcgat cgggtgcccg gtgtgctctc gccaacatcg
    17521 agcagaagcg acctccagcg tttgctgcaa aagcgcacgc agctatcaat agcatagtgc
    17581 atcggatgat cggcgagcga tcgtaggcgc acttgtcgca ggtcgcgcgc gctatcattg
    17641 acagtttccg catttcaccg tcggcccgcg ggcctgtcca gcgccagtgc gtctcaattc
    17701 catcaagctg tcgggcttca agtccttcgc cgaacccacc aacttcatgc tgcccggcca
    17761 actggtcggc gtggtgggcc ccaatggctg cggcaaatcc aacatcatgg acgcggtgcg
    17821 ctgggtgctg ggcgaatcca aggcgtccga gctgcgcggc gagtcgatgc aggacgtgat
    17881 cttcaacggc accaccacgc gcaagccggc gtcgcgctcg tcggtggagc tggtgttcga
    17941 caacagcgac caccgcgccg gcggccagtg gagccagttc accgagattt cggtcaagcg
    18001 cgtgctgacg cgcgacggca ccagcagcta ctacatcaac aaccagccgg tgcgccggcg
    18061 cgacgtgcag gacgtgttcc tgggcaccgg cctggggccg cgcgcctacg ccatcatcgg
    18121 ccagggcacc atcagccgca tcatcgagtc gcgccccg
//
LOCUS       DAOQTZ010000054        17966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3582_length_17966_cov_14.127575, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000054 DAOQTZ010000000
VERSION     DAOQTZ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17966
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3582_length_17966_cov_14.127575"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..2795
                     /gene="polA"
                     /locus_tag="PKN51_07935"
     CDS             24..2795
                     /gene="polA"
                     /locus_tag="PKN51_07935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNT85067.1"
                     /translation="MSSDPTPATAPRLLLVDGSSYLYRAFHAMPDLRADPNDRHSQPT
                     GAIRGMINMLNALRKDYPDAPYAACVFDAPGKTFRDELYPDYKAQRAPMPDDLRSQIA
                     PIHEVVRLLGWKVLAVPGVEADDVIGTLAQAAAGQGVEVVISSGDKDLAQLVTPHIVI
                     VDTMTGKRRDMAGVQAEFGVRPDQMLDYQVLLGDAIDNVPGVDKVGPKTAARWLGEYG
                     SLDAIVAQADRIKGAAGDNLRRALAWLPTGRRLLQIRTDCDLTGHVDGLPALDSIAAG
                     ASDTSALAEFYDKYGFRTLARALRPAAAGDAAPNAAPVPAAAATGGHDVAYETILDWP
                     AFERWLARIEAAELTALDTETTSIDEQQARIVGLSLSVRPREAAYIPLAHDGPGAPAQ
                     LPLGEVLARLKPWLENPARTKLGQHVKYDRHVLANHGIEVAGYVHDTMLQSYVLEVHR
                     PHGLASLAERHLGRRGIDYEALCGKGVHQIPFAQVPIDKAAHYACEDADQTLEVHLAL
                     WPQVQAEPGLARIYALEIATSEVLYRIERHGVLIDAAQLQRQSHELGRRILELERQAH
                     ELAGQPFNLGSPKQIGEIFFDKLGLPVVKKTATGKPSTDEEVLEKLAEDYPLPARILE
                     HRGLAKLKSTYTDKLPQLVDPVSGRVHTHYAQAVAVTGRLASNDPNLQNIPVRTPEGR
                     RIREAFIAPPGHVVVSCDYSQIELRIMAHLSDDAALLAAFHAGMDVHRATAAEVFGVA
                     PEQVSSEQRRYAKVINFGLIYGMSSFGLARNLGIDNTAARNYIDRYFERYPGVRRYMD
                     DTRQAAKARGFVETVFGRRLYLPEINSPNGPRRAAAERAAINAPMQGTAADLIKMSMV
                     AVQAAIDQQRRASRMIMQVHDELVLEVPEAELAWARAEVPRLMAGVAQLKVPLLAEVG
                     MGPNWEQAH"
     gene            complement(2872..3891)
                     /locus_tag="PKN51_07940"
     CDS             complement(2872..3891)
                     /locus_tag="PKN51_07940"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNT85068.1"
                     /translation="MSSPISLSRYLVEKQRIAGHIPAQLRLLLEVVARACKSISHAVN
                     KGALGGVLGSAGSQNVQGEVQKKLDIIANEVLIDANEWGGHLAAMASEEMEAIHVVPN
                     RYPKGEYLLLFDPLDGSSNIDVNVSIGTIFSVLKMPEGDRGVEEEDFLQPGSHQVAAG
                     YCVYGPQTTLVLTVGDGVAMFTLDRELGSFVLTQDDLRIPDDTQEFAINMSNLRHWDA
                     PVRRYIDECLAGKDGPRGKNFNMRWVASMVADVHRIMMRGGVFMYPWDKREPNKPGKL
                     RLMYEANPMSWLIEQAGGAATNGRQRILDVQPQQLHERVSVILGSKNEVERVTRYHLD
                     ADAAS"
     gene            complement(3888..6647)
                     /gene="pepN"
                     /locus_tag="PKN51_07945"
     CDS             complement(3888..6647)
                     /gene="pepN"
                     /locus_tag="PKN51_07945"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNT85069.1"
                     /translation="MREGQTAVTRRQDYAPPAFWIDSVELTIDLDPAKTRVLSRLRLR
                     RNPDVAAQPLRLDGDELNLSRVMLGGQACSFRLDDQTLVLEQLPADHAPFELEIFTTC
                     APEKNSKLMGLFMSGGDFFTQCEAEGFRRITYFLDRPDVMATYTVTLRADKARWPVLL
                     ANGNLVDAGELDDGRHFATWHDPHRKPCYLFALVAGNLVAREQRITTRTGSEHLLQVF
                     VRPGDLDKTEHAMRSLIHAVAWDEARFGLPLDLERFMVVATSDFNAGAMENKGLNIFN
                     TRYVLAKPSTATDADYDAIESVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEF
                     SEDLAGTPSARAVKRIENVIALRAAQFPEDASPMAHPVRPDEYLEISNFYTTTIYEKG
                     AEVVRMIQSLLGRAGFRRGMDLYFQRHDGQAVTCDDFAQAMADANPGSTLAPLLPQFK
                     RWYSQAGTPVLQATGQYDAQARRYTLTLSQSCPATPGQPHKAPFVIPVRLGLVGADGR
                     ELPLTLSQGELLHGDTCVLTEPQTTLVFSEVDQPPVPSLLRGFSAPVELRGLDADEQL
                     LHLLAYDSDPFNRWDAGQRLGVRIALENIASGADQVSARPLKDNYLQALRAVLRQPQL
                     DPAFKALVLKLPDEDYIADQLDVVDPQRIHAVRDAMQRQLATALRDDWQWAWEQHHDT
                     GAYRPDPHSSGRRALAGLALAMLCLAASEQGDSVWPGKAYQRVKDAGNMTDRLNALGA
                     LVGCGHELGAHALSRFHRLFRHEPLALDKWFALQARAPDRRGGALPTVRLLMKHPDFS
                     LRNPNRARSLIFAYCHNNPAGFHRRDAAGYLFWADRVLELDTLNPQVAARLARAMDAW
                     ARLAEPYRSAAREAIARVAARPDLSNDVREVVTRALAGQAPALAADAEARTTETPDTQ
                     QKGSA"
     gene            complement(6732..7836)
                     /gene="prfB"
                     /locus_tag="PKN51_07950"
     CDS             complement(join(6732..7754,7756..7836))
                     /gene="prfB"
                     /locus_tag="PKN51_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123395.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNT85070.1"
                     /translation="MEAERLNQISAQIDDLRARTDALRGYLDYAGKAERLRTVNAALE
                     DPAVWNDPRKAQELGKEKKQLDGVVVTLDELTQGLTDSAELFELSREENDDASLQAIG
                     SDVEKLARTVEELEFRRMFNQPADPNNCFIDIQAGAGGTEACDWASMLLRQYLKYAER
                     KDFNTEIQEETPGDSAGIKSATIKVEGEYAYGLLRTETGVHRLVRKSPFDSSGGRHTS
                     FASVFVYPEVDDSIEIDINPADVRTDTYRASGAGGQHINKTDSAVRLTHLPTGIVVQC
                     QDSRSQHSNRDVAWQRLRARLYDHEMRKRMEDQQKLEDSKTDVGWGHQIRSYVLDNSR
                     IKDLRTGVEISATQKVLDGDLDPFIEASLKQGV"
     gene            complement(8045..8974)
                     /locus_tag="PKN51_07955"
     CDS             complement(8045..8974)
                     /locus_tag="PKN51_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT85071.1"
                     /translation="MTYVPHRPARSESLPLRQIHVHVNCWGRPQPGSTPLVLVHGWMD
                     VGASYQFMVDALSAEFADQRLIIAPDWRGFGDTTLAAPCDHYVLADYLGDLDALLDHY
                     APNGQPVDLVGHSMGGNVVMQYAGVRPARIRRLINLEGFGMPASRPTQAPRRLAQWLD
                     ELQQLRRGEISLKTYPDADAVAQRLMKTNPRLPADKAAWLARQWARPQADGQWRVLGD
                     AAHKIVNPTLFRVDEMLALYAAITAPVLVIEGSDDSLSQWWKGRYTLAEFHQRLQAVP
                     DLRRQQLPDSGHMLHHDQPERLAAALQDFLSPP"
     gene            9134..10453
                     /locus_tag="PKN51_07960"
     CDS             9134..10453
                     /locus_tag="PKN51_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT85072.1"
                     /translation="MKHKLLVMALGAALAAPAAIAGTVTVLTSFPKELTTAYKKAFES
                     AHPGITVEILNKNTTASVAYIRELPEGQRPDVMWASAPDAFEVLSSFKLLQSAPEVVN
                     KSAPAKIGNYPLNDPKGMYYGQALAGYGISWNTRYLKAHKLTAPKEWSDLVRPEYFGH
                     IAISSPSRSGTTQLTVETILQGEGWDKGWTQLLQMMGNAAAVTDRSFAVPDGVNNGQY
                     GIGIVIDFLALAGKYSGYPVDFTYPSMTAVVPANIALIAGAKNADEAKKFMAYTMSVP
                     GQQLLFDPKIGRLPMLPYSMLKPPAGYPVPQDIAKRAKVQFNTELSGQRYPVVISLFD
                     QMVTFRLKELQAATKSIHEATAALKARPNARGSELLAQARSLAYTSLVGADNVKNPEF
                     LELFRKSRRDVVVSKQLTGMEQMWSEKARANYERAKQLADEARGLAK"
     gene            10512..12248
                     /locus_tag="PKN51_07965"
     CDS             10512..12248
                     /locus_tag="PKN51_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNT85073.1"
                     /translation="MSASTVAPGRAGPSARPRPRVGPGAWLAFGLILAFLLLFLIVPV
                     GTVIYTAFVNETGSLTVGHFANFFGQLVFRESFFNSLGVALASTFFASVIAVPLAYLT
                     VRFEFRGALLIQTLGVLPLIMPPFVGAVALQLIFGRSGSVNLLLDDWLGFTIPIMDGL
                     VGVTFVESIHYFPFILLNLVAAMRNIDGAMEESALNLGARGWRLFRRVIFPLAMPGYL
                     AGAALVFVKVFDDLGTPLVMGVTNMLAPQAYLRITSVGVDDPLGYVISVIMIAFSILA
                     LWLAARVMKGRDYATLQKGGGALHKRRLTAWEAVLAYGWIALVLLVVLAPHAGILLMS
                     FAQVWSFSVLPDSYTLAHYATVFSDASGMIGNTLLYCLLAAGLDVVIGTAIAYLILRT
                     RLPARQWLDWLASAALAIPGLVLAIGYLRLFRGMHVPFSDQLVIHSWVLIMLAYAVRR
                     LPYALRSCMAALQQVHVSLEEAAQSLGASRLSTIRRVVVPLMAGGILAGFVTSFITAA
                     VELSATLLLVSSESQAPMSLGIYLYMQSIAGRGPGAALGVLAVLVVGLGTYFSHRFVE
                     RSRVTTSASTKA"
     gene            12251..13336
                     /locus_tag="PKN51_07970"
     CDS             12251..13336
                     /locus_tag="PKN51_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85074.1"
                     /translation="MNKASLECRHISLSYGSTPVLRDINLKIEPGEFFALLGPSGSGK
                     STLLRLIAGFNQHQSGELLIDGQDISGVPPHARHIGMVFQNYALWPHMTVWDNVAFGL
                     VERRESRDAIRRKVGAALELVGLADYAKRRPGQLSGGQQQRVALARTIVIEPKLLLLD
                     EPLSNLDKNLRVQMREELKNLQRTLGLTTIFVTHDQEEAMTTADRMAVLDQGVLQQVG
                     TAAGLYDYPDNRFVAGFVGTANLLEGELKMAPDGALQFHARGLGPLTVARPDAAPTLG
                     AATLALRPHQVSIADPQQAGDAARLWLDGQVESAEFLGEFSRYRVRVGELALLSDQPH
                     YAGVPLFPAGAPVRLGIDPRQLRVLTS"
     gene            13396..13893
                     /locus_tag="PKN51_07975"
     CDS             13396..13893
                     /locus_tag="PKN51_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HNT85075.1"
                     /translation="MAKSKSAHVSETPATALLRAHGIAFTEHPYEYVEHGGAEHSAEV
                     LGFDPFSVVKTLIMQDQDARPLIVLMHGNRKVSTKNLARQIGAKSVEPCKPEVANRHS
                     GYLVGGTSPFGTRRQMSVYIEESILELPRIAINGGRRGYLVAIEPQVCVRLLGAQPVR
                     CAIEL"
     gene            13973..14596
                     /gene="plsY"
                     /locus_tag="PKN51_07980"
     CDS             13973..14596
                     /gene="plsY"
                     /locus_tag="PKN51_07980"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNT85076.1"
                     /translation="MILPLLATLAAYLIGSLSFAVIVSRAMGLQDPRSYGSGNPGATN
                     VLRSGSKAAAILTLLLDAAKGWLPVVLVRAFGADLGLGAGTQALVGLAAFLGHLYPVF
                     FRFQGGKGVATAAGVLLAFAPWLGVATLVTWLIIAVFFRYSSLASIVAALFAPAYYLL
                     GDGVAWTASGYKTLALMVMGVLLIWRHRENIRRLLAGTESKLGSKKK"
     gene            14593..14946
                     /locus_tag="PKN51_07985"
     CDS             14593..14946
                     /locus_tag="PKN51_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNT85077.1"
                     /translation="MSAIQRHGVGARLSEAAVFNGVVYLAGMVPEGGARDIRAQTADV
                     LAQIDAQLAACGSDRTRILRAQIFLADIADIAAMNEVWDAWVVPGHAPPRATVQAALA
                     DPAWRIEVVVTAAQA"
     gene            complement(15004..15657)
                     /locus_tag="PKN51_07990"
     CDS             complement(15004..15657)
                     /locus_tag="PKN51_07990"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNT85078.1"
                     /translation="MAALCTPWRTLLAGALLLPTWAGAQNVSLHGLLGQRALLIVDDQ
                     APRAVAPGETHLGVQVLSTGDDRAVVRVGGQRLTLRVGESPASVGGAPAPAGSDRVAL
                     TADARGHFFVAGSINQRPTQFMVDTGASVVAIGQAEADRLGLNYRAGHSVTLRTANGN
                     APGWALKLASLRIGDVTAYEVDAVVTPAAMPAVLLGNSFLNRFNMRRDGATMLLVKR"
     gene            complement(15665..16150)
                     /locus_tag="PKN51_07995"
     CDS             complement(15665..16150)
                     /locus_tag="PKN51_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNT85079.1"
                     /translation="MPSFDTVCDPDMVEVRNAVDNAAKEIGTRFDFKGTSAAIELKDK
                     EITLLGDAEFQLQQVEDILRGKLTKRGVDVRFLDKGKVEKIGGDKVRQLVKVKSGIAT
                     EDGKKIQKLIKESKLKVQAAIQGDAVRVTGAKRDDLQAVMALLRKDMAELPLSFNNFR
                     D"
     gene            16194..17246
                     /gene="murB"
                     /locus_tag="PKN51_08000"
     CDS             16194..17246
                     /gene="murB"
                     /locus_tag="PKN51_08000"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNT85080.1"
                     /translation="MFVERNVSLQPLNSFGIAARAAHMVRVRSEDEVRAVLADAGLTA
                     AGLFVLGGGSNIVLTGDLRALVLKVEVPGRRVVDQTARHTIVEAGAGESWHDFVGWTL
                     DQGLPGLENLALIPGTVGASPVQNIGAYGVELQDRFHELDAIDLATGELFTLNAAQCG
                     FGYRDSVFKHAPAQPGDFGLAGRALILRVRLALPRAWRPVLGYLDLERRMQETGISAP
                     DARQIHDWIVAIRRAKLPDPAVIGNAGSFFKNPTVTPEQCQDIIGRDPKLVHYPMPDG
                     SVKLAAGWLIDACGWKGKRMGNAAVYDKQALVLVNQGGSQHPATGGEVMTLARAIQTS
                     VYERFGIRLEPEPVVV"
     gene            17265..17729
                     /locus_tag="PKN51_08005"
     CDS             17265..17729
                     /locus_tag="PKN51_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85081.1"
                     /translation="MRAIELFHLRRVRDKPRALALIAAHARLSGDQALAVLHAAIGGG
                     RPRLCLSDDEAARACIVALAPAGFVARFAPGADFDLAQHAQQALMAALPACAPDLAAQ
                     AGARLLHDDWPEALALALQHLRMHRPAQHPGRRRLEQAAIDTGLVRGVPGRT"
BASE COUNT         2601 a         6060 c         6303 g         3002 t
ORIGIN      
        1 tgcgccgcag gcacaatgcc gcgatgagca gcgaccccac acccgccacc gcgccccgcc
       61 tgctgctggt cgatggctcc agctacctgt accgggcgtt tcacgccatg cccgatctgc
      121 gcgccgatcc gaacgatcgt cacagtcagc ccaccggcgc gatccgcggc atgatcaaca
      181 tgctcaacgc gctgcgcaag gactaccccg acgcgcccta tgcggcctgc gtgttcgacg
      241 cccccggcaa gaccttccgc gacgagctgt accccgacta caaggcccag cgcgcgccca
      301 tgcccgacga cctgcgcagc cagatcgcgc cgatccacga ggtggtgcgc ctgctgggct
      361 ggaaggtgct ggcggtgccg ggcgtggagg ccgacgacgt gatcggcacg ctggcgcagg
      421 cggcggcggg gcagggcgtg gaggtggtga tatccagcgg cgacaaggac ctggcgcaac
      481 tggtcacgcc gcacatcgtg atcgtcgaca ccatgaccgg caagcgccgc gacatggccg
      541 gtgtgcaggc cgagttcggc gtgcgtcccg accagatgct ggactaccag gtgctgctgg
      601 gcgacgccat cgacaacgtg ccgggggtgg acaaggtcgg ccccaagacc gccgcccgct
      661 ggctgggcga atacggctcg ctcgacgcca tcgtggcgca ggccgaccgc atcaagggtg
      721 cggccggcga caacctgcgc cgcgcgctgg cctggctgcc gaccgggcgc cggttgctgc
      781 agatccgcac cgactgcgat ctgacaggcc atgtcgatgg cctgcctgct ctggattcaa
      841 tagctgctgg cgcaagcgac acaagcgcgc tggccgagtt ttatgacaaa tacgggttcc
      901 gtacgctggc tcgcgcgctg cgccccgccg cagccggcga cgccgccccg aacgccgcgc
      961 cggtgccggc cgcggctgcg acgggcgggc acgacgtggc gtacgaaacc atcctggact
     1021 ggccggcctt cgagcgctgg ctggcgcgca tcgaagcggc cgaactgacc gcgctggaca
     1081 ccgaaaccac ctcgatcgac gagcagcagg cgcgcatcgt cggtctttcg ctcagcgtgc
     1141 ggccgcgtga ggccgcctac atcccgctgg cgcacgacgg gcccggcgcc cccgcgcagc
     1201 tgccgctggg cgaggtgctg gcgcgcctga agccgtggct ggaaaacccg gcccgcacca
     1261 agctcggcca gcacgtcaag tacgaccgcc atgtgctggc caaccacggc atcgaggtgg
     1321 ccggttacgt gcacgacacc atgctgcaga gctatgtgct cgaagtgcat cgcccgcatg
     1381 gcctggccag cctggccgaa cgccacctgg ggcgccgcgg catcgactac gaagcgctgt
     1441 gcggcaaggg cgtgcaccag ataccgttcg cccaggtgcc gatcgacaag gccgcccact
     1501 acgcctgcga ggacgccgat cagacgctgg aagtgcatct ggcgctgtgg ccccaggtgc
     1561 aggccgagcc ggggctggcg cgcatctatg cgctggagat cgccaccagc gaagtgctct
     1621 accgcatcga gcgccacggc gtgctgatcg acgctgcgca gctgcagcgc cagagccacg
     1681 agctgggccg ccgcattctg gagctggagc gccaggcgca cgagctggcg ggccagccct
     1741 tcaacctcgg ctcgcccaag cagatcggcg agatcttctt cgacaagctg ggtctgccgg
     1801 tggtcaagaa gaccgccacc ggcaagccca gcaccgacga ggaggtgctg gaaaaactgg
     1861 ccgaagacta cccgctgccg gcgcgcatcc tggagcaccg cggcctggcc aagctcaaga
     1921 gcacctacac cgacaagctg ccgcagctgg tggacccggt cagcggccgc gtgcacaccc
     1981 actatgcgca ggcggtggcg gtgaccgggc gcctggccag caacgacccc aacctgcaga
     2041 acatcccggt gcgcacgccc gagggccggc gcatccgcga agccttcatc gcgccccccg
     2101 gccatgtcgt cgtcagctgc gactacagcc agatcgaact gcgcatcatg gcgcacctga
     2161 gcgacgatgc ggcgctgctg gccgccttcc acgcgggcat ggacgtgcac cgcgccaccg
     2221 ccgccgaggt gttcggcgtc gcgcccgagc aggtcagcag cgagcagcgc cgttatgcca
     2281 aggtgatcaa cttcggtctc atctacggca tgagcagctt cgggctggcg cgcaacctcg
     2341 gcatcgacaa cacggcggcg cgcaactaca tcgaccgcta tttcgagcgt tacccgggcg
     2401 tgcggcgcta catggacgac acgcgccagg cggccaaggc gcgcggcttc gtcgaaaccg
     2461 tgttcggccg ccggctgtac ctgcccgaga tcaacagccc caacgggccg cgccgcgccg
     2521 ccgccgagcg cgccgccatc aacgctccca tgcagggcac ggcggccgac ctgatcaaga
     2581 tgagcatggt ggcggtgcag gctgccatcg accagcagcg gcgcgccagc cgcatgatca
     2641 tgcaggtgca tgacgagctg gtgctggaag tgcccgaggc cgagctcgcg tgggcgcgcg
     2701 ccgaggtgcc gcgcctgatg gccggtgtgg cccagctcaa ggtgccgctg ctggccgagg
     2761 tcggcatggg gccgaactgg gagcaggcgc actgagggtg ttctgatcgc tatcagcctc
     2821 cagcgcccat ccagcaagcg ccgacagcta tgattttgag agcgtctcgg ctcagctggc
     2881 ggcgtcggcg tcgaggtgat agcgggtcac gcgctcgact tcgtttttgc tgcccaggat
     2941 cacgctgacg cgttcgtgca gttgctgcgg ctgcacgtcc aggatgcgct ggcggccgtt
     3001 ggtggcggcg ccgccggcct gctcgatcag ccagctcatc gggttggcct cgtacatcag
     3061 gcgcagcttg ccgggcttgt tgggttcgcg cttgtcccag gggtacatga agacgccgcc
     3121 gcgcatcatg atgcggtgca cgtcggccac catgctggcg acccagcgca tgttgaagtt
     3181 cttgccgcgt ggcccgtcct tgccggccag gcattcgtcg atgtagcggc gcaccggcgc
     3241 gtcccagtgg cgcaggttgc tcatgttgat ggcgaactcc tgcgtatcgt cgggaatgcg
     3301 caggtcgtcc tgggtcagca cgaagctgcc cagttcgcgg tccagggtga acatggccac
     3361 gccgtcgccc accgtcagca ccagggtggt ctgcggccca tagacgcagt aaccggccgc
     3421 cacctggtgg ctgccgggtt gcaggaagtc ttcttcctcg acgccgcggt cgccttcggg
     3481 catcttcagg acgctgaaga tggtgccgat gctgacgttg acgtcgatgt tgctggagcc
     3541 gtccagcggg tcgaacagca gcaggtattc gcccttcgga taacggttgg gcaccacgtg
     3601 gatggcttcc atctcctcgc tggccatggc cgccaggtgg ccgccccatt cgttggcgtc
     3661 gatcagcacc tcgttggcga tgatgtccag ctttttctgc acctcgccct gcacgttctg
     3721 gctgccggcg ctgcccagca cgccgcccag ggcgcccttg ttgacggcgt ggctgatgct
     3781 cttgcaggcg cgtgccacca cttcgagcag caggcgcagc tgggccggaa tgtggccggc
     3841 gatgcgctgc ttttcgacca ggtagcgcga cagggagatg ggcgagctca tgcggatcct
     3901 ttctgctgcg tgtcgggggt ttcggtggtg cgcgcctcgg cgtcggcggc cagcgccggc
     3961 gcctggccgg cgagggcgcg cgtgacgact tcacgcacgt cgttgctcag gtcgggcctg
     4021 gcggcgacgc gggcgatggc ctcgcgggcg gcactgcggt atggctcggc cagacgtgcc
     4081 caggcgtcca tggcacgcgc cagccgcgcc gccacctgcg ggttgagggt atcgagctcc
     4141 agcacgcggt cggcccagaa caggtagccg gcggcgtcgc gccggtgaaa gccggccggg
     4201 ttgttgtggc agtaggcaaa gatcaggctg cgcgcgcggt tcgggttgcg cagactgaag
     4261 tcggggtgct tcatcagcag ccgcaccgtc ggcagcgctc cgccgcggcg gtcgggcgca
     4321 cgcgcctgca gggcgaacca tttgtcgagc gccagcggct catggcggaa caggcggtga
     4381 aagcgcgaca gggcatgcgc gcccagctcg tgcccgcagc cgaccagggc gcccagcgcg
     4441 ttcaggcggt cggtcatgtt gccggcgtcc ttgacgcgct ggtaggcctt gccgggccag
     4501 acgctgtcgc cctgttcgct ggcggccaga cacagcatgg ccagcgccag cccggccagc
     4561 gcgcgccggc cgctggagtg cgggtcgggg cggtaggcgc cggtgtcgtg gtgttgctcc
     4621 caggcccatt gccagtcgtc gcgcaaggcg gtggccagct gccgctgcat ggcgtcgcgc
     4681 acggcgtgga tgcgctgcgg atcgaccacg tcgagctgat cggcgatgta gtcctcgtcg
     4741 ggcagcttca gcaccagggc tttgaaggcg gggtccagct ggggctggcg cagcaccgcg
     4801 cgcagggcct gaagataatt gtctttcagg ggtctggcgc ttacctggtc tgcaccggaa
     4861 gctatgtttt caagagcaat gcggacgccc aggcgctggc cggcgtccca gcggttgaag
     4921 gggtcgctgt cgtacgccag caggtgcagc agctgctcgt cggcgtccag tccgcgcagt
     4981 tcgaccggcg ccgagaagcc gcgcagcagc gacggcacgg gcggctgatc gacctcgctg
     5041 aagaccaggg tggtctgcgg ctcggtcagc acgcaggtgt cgccgtgcag cagctcgccc
     5101 tggctcagcg tcagcggcag ctcgcgcccg tcggcgccca ccaggccgag ccgcaccgga
     5161 atcacgaacg gcgccttgtg cggctggccg ggcgtggccg ggcagctttg cgacagcgtc
     5221 agggtgtagc ggcgcgcctg ggcgtcgtac tggccggtcg cctgcagcac cggcgtgccg
     5281 gcctggctgt accagcgctt gaactgcggc agcagaggcg ccagtgtgct gcccgggttg
     5341 gcgtcggcca tggcctgggc gaagtcgtcg caggtcacgg cctggccgtc gtggcgctgg
     5401 aagtacaggt ccatgccgcg gcgaaagccg gcacggccca acaggctttg aatcatgcgc
     5461 accacctcgg cgcctttttc gtagatggtg gtggtgtaga agttgctgat ttccaggtat
     5521 tcgtcgggcc gcaccgggtg ggccatgggg ctggcgtcct cggggaactg cgcggcgcgc
     5581 agggcgatga cgttttcgat gcgtttgacg gcgcgtgcgc tgggggtgcc ggccaggtcc
     5641 tcgctgaatt cctgatcgcg gaacacggtc aggccttcct tgagcgagag ctggaaccag
     5701 tcgcggcagg tgacgcggtt gccggtccag ttgtggaagt actcgtggcc gatcacgctt
     5761 tcgatcgcgt cgtagtcggc gtcggtggcg gtcgagggct tggccagcac ataccgggtg
     5821 ttgaagatgt tcaggccctt gttttccatg gcgccggcgt tgaagtcgct ggtggccacc
     5881 accatgaagc gctccaggtc cagcggcagg ccgaaacgcg cctcgtccca cgccacggcg
     5941 tggatcagcg agcgcatggc gtgttcggtc ttgtccaggt cgcccgggcg cacgaagacc
     6001 tgcagcaggt gctcgctgcc ggtgcgtgtg gtgatgcgct gctcgcgcgc caccaggttg
     6061 ccggccacca gggcgaacag gtagcagggc ttgcggtgcg ggtcgtgcca ggtggcgaag
     6121 tggcgcccat cgtccagctc gccggcgtcc accaggttgc cgttggccag cagcaccggc
     6181 cagcgcgcct tgtcggcgcg cagggtgacg gtgtaggtgg ccatcacgtc gggccggtcg
     6241 aggaagtagg tgatgcggcg aaagccttcc gcttcgcact gggtgaagaa gtcgccgccg
     6301 ctcatgaaca ggcccatcag cttgctgttc ttctcgggcg cgcaggtggt gaagatttcc
     6361 agctcgaacg gcgcgtggtc ggccggcagc tgctccagca ccagggtctg gtcgtccaga
     6421 cggaacgaac aggcctgacc gcccagcatc acgcgcgaga ggttgagctc gtcgccgtcc
     6481 aggcgcagcg gctgcgccgc cacgtccggg ttgcggcgca gccgcaggcg gctcagcacc
     6541 cgggtcttgg ccgggtccag atcgatggtc agctcgacgc tgtcgatcca gaaggcgggc
     6601 ggggcgtagt cctggcgccg ggtcacggcg gtttgtcctt cacgcatggc ggatcctgtg
     6661 ttgctctgat tgtgatagct gctcgcgctc tcccagtctg cgcgagaggc cctaaaggct
     6721 tgcaaatggg cttacacgcc ctgtttcagg ctggcctcga tgaaggggtc gaggtcgccg
     6781 tccagcactt tctgcgtggc gctgatttcg acgccggtgc gcagatcctt gatgcggctg
     6841 ttgtccagca cgtagctgcg gatctgatgg ccccagccga cgtcggtctt gctgtcttcc
     6901 agcttctgct ggtcttccat gcgcttgcgc atttcgtggt cgtacaggcg cgcgcgcagg
     6961 cgctgccagg cgacgtcgcg attgctgtgc tggctgcggc tgtcctggca ctgcaccacg
     7021 atgccggtgg gcaggtgcgt cagccgcacc gccgagtcgg tcttgttgat gtgctggcca
     7081 ccggcgccgc tggcgcggta ggtgtcggtg cgcacgtcgg ccgggttgat gtcgatctcg
     7141 atcgaatcgt ccacttcggg gtagacgaac acgctggcaa agctggtgtg gcgcccgccc
     7201 gaggaatcaa agggcgactt gcgcaccagg cggtgcacgc cggtttcggt gcgcagcagg
     7261 ccgtaggcgt attcgccctc caccttgatg gtggcgctct tgatgccggc gctgtcgccc
     7321 ggggtttctt cctggatttc ggtgttgaaa tccttgcgct cggcgtattt caggtactgg
     7381 cgcagcagca tgctggccca gtcgcaggcc tcggtgccgc cggcgccggc ctggatgtcg
     7441 atgaagcagt tgttggggtc ggccggctgg ttgaacatgc ggcggaattc gagctcctcc
     7501 accgtccggg ccagcttttc gacgtcgctg ccgatggcct gcaggctggc gtcgtcgttt
     7561 tcctcgcggc tgagctcgaa cagctcggcg ctgtcggtca ggccctgggt cagctcatcg
     7621 agcgtgacca ccacgccatc gagctgcttt ttttccttgc ccagctcctg ggccttcctg
     7681 gggtcgttcc agaccgccgg gtcttccagt gcggcgttga cggttctcag gcgttcggcc
     7741 ttgccggcgt agtcaaagat acccccgtag cgcgtcggtg cgcgcacgca ggtcgtcgat
     7801 ttgggcgctg atctggttga ggcgttctgc ttccatgggt tgctgtcctg tgctgtggct
     7861 ggctgcgcgc ccggtggctg gggcgcgagg cgtggtgtgt gacctggggt tgaacgcaag
     7921 gcgccggggc cacctggccc ggcggcatgc gccgatcggg ggtggggcag gccgtcgtca
     7981 acccgtgagg gcggcatttt ctcacgccgg gcgggtgatc ggccgcccgc cgcgcccgcc
     8041 ggcgttacgg cggtgacaga aagtcctgca gcgcggcggc caggcgctcg ggctggtcgt
     8101 ggtgcagcat gtggccgctg tcgggcagct gctgccggcg cagatccggc accgcctgca
     8161 gccgctgatg gaattccgcc agcgtgtagc gccccttcca ccactgcgac aggctgtcgt
     8221 cgctgccctc gatcaccagc accggcgcgg tgatggcggc atacagggcc agcatctcat
     8281 cgacccgaaa cagggtcggg ttgacgatct tgtgcgccgc atcgcccaaa acccgccact
     8341 ggccatcggc ctgcggccgc gcccactggc gcgccagcca ggcggccttg tcggccggca
     8401 ggcgcgggtt ggtcttcatc aggcgctggg ccacggcgtc ggcgtcgggg taggtcttga
     8461 ggctgatttc gcctcggcgc agctgttgca gctcgtccag ccattgcgcc agccggcgcg
     8521 gcgcctgcgt cggccggctg gccggcatgc cgaagccttc cagattgatc agccggcgga
     8581 tgcgcgccgg ccgcaccccg gcgtactgca tcaccacgtt gccgcccatg ctgtggccga
     8641 ccaggtcgac cggctggccg ttcggggcgt agtggtcgag cagggcgtcc agatcgccca
     8701 ggtagtcggc cagcacgtag tggtcgcagg gggcggccag cgtggtgtcg ccaaaaccgc
     8761 gccagtcggg cgcgatgatc aggcgctggt cggcaaactc ggcgctcagc gcgtcgacca
     8821 tgaactggta ggacgccccc acgtccatcc agccgtgcac cagcaccagc ggcgtgctgc
     8881 ccggctgggg tcggccccag cagttgacgt ggacatggat ctggcgcagg ggcagcgatt
     8941 cgctgcgcgc gggacggtgc ggcacgtaag tcatgggcgg cgattatttc ccggccgccc
     9001 gcagcgtgcc ggtctggggg ttaacccgct cagacatcgc gcgcttgtca cgtaaaaacc
     9061 cggcgtggcg ggttggccgg tgccgagcgg acggcgccgc gacccggcaa tcgttgtcga
     9121 gaggagatga aacatgaaac acaagcttct ggtgatggcc ctgggcgcgg cgctggcggc
     9181 gccggccgcg atcgccggca cggtgacggt gctgacctcg ttccccaagg agctgaccac
     9241 cgcctacaag aaggcgttcg agtcggccca cccggggatc acggtggaaa tcctgaacaa
     9301 gaacaccacc gcgtcggtcg cctacatccg cgagctgccc gagggccagc gccccgacgt
     9361 catgtgggcc agcgcgcccg acgccttcga ggtgctgtcc agcttcaagc tgctgcaaag
     9421 cgcgcccgag gtggtgaaca agagcgcgcc ggccaagatc ggcaactacc ctctcaacga
     9481 ccccaagggc atgtactacg gccaggcgct ggcgggctac ggcatttcgt ggaacacgcg
     9541 ctacctcaag gcgcacaagc tcacggcgcc gaaggaatgg agcgacctgg tgcgtcccga
     9601 gtacttcggc catatcgcca tctcgtcgcc ctcgcgttcg ggcacgaccc agctcacggt
     9661 ggaaaccatc ttgcagggtg agggctggga caagggctgg acgcagttgc tgcagatgat
     9721 gggcaacgcc gccgccgtca ccgaccgcag ctttgccgtg cccgacggcg tgaacaacgg
     9781 gcagtacggc atcggcatcg tgatcgactt tctggcgctg gccggcaagt attcgggcta
     9841 tccggtcgac ttcacctacc cgagcatgac cgccgtggtg ccggccaaca ttgcgctcat
     9901 cgccggtgcc aagaacgccg acgaagccaa gaaattcatg gcctacacca tgtcggtgcc
     9961 cggccagcag ctgctgttcg accccaagat cggtcgcctg cccatgctgc cctactcgat
    10021 gctcaagccc ccggccggct accctgtgcc gcaggacatc gccaagcgcg ccaaggtgca
    10081 gttcaacacc gagctgtcgg gccagcgcta cccggtggtg atcagcctgt tcgaccagat
    10141 ggtgacgttt cgcctgaagg agctgcaggc cgccaccaag tccatccatg aagccacggc
    10201 cgccctcaag gccaggccca acgcgcgcgg cagcgagctg ctggcgcagg cgcgcagcct
    10261 ggcctacacc tcgctggtgg gcgccgacaa cgtgaagaac cccgagttcc tggagctgtt
    10321 ccgcaagagc cggcgcgatg tggtggtgtc caagcagctc acgggcatgg agcagatgtg
    10381 gtcggagaag gcgcgcgcca actacgaacg cgccaaacag ctggccgacg aagcgcgcgg
    10441 cctggccaag tgagcggcgc gccgtcgccc cgtccggcgc cgccagtcgg cgggcgccca
    10501 agaggagccg tatgagcgca tccaccgtgg caccgggccg tgccgggccc agcgcgcggc
    10561 cgcgcccgcg tgtggggccg ggcgcctggc tggcgtttgg cctgatcctg gcgtttctgc
    10621 tgctgtttct gatcgtgccc gtgggcacgg tgatctacac cgccttcgtc aacgagacgg
    10681 gctcgctcac cgtgggccac tttgccaatt tcttcggcca gctggtgttt cgcgagtctt
    10741 tcttcaacag cctcggggtg gcgctggcca gcaccttctt tgccagcgtc atcgccgtgc
    10801 cgctggcgta cctgacggtg cgcttcgagt tccgcggcgc gctgctgatc cagaccctgg
    10861 gcgtgctgcc gctcatcatg ccgcccttcg tgggcgcggt ggcgctgcaa ctgatcttcg
    10921 ggcgcagcgg ctcggtgaat ctgctgctcg atgactggct gggcttcacc attccgatca
    10981 tggacggtct ggtgggcgtg acctttgtcg agagcatcca ctacttcccg ttcatcctgc
    11041 tcaacctggt ggcggccatg cgcaacatcg acggcgccat ggaggagtcg gcgctgaacc
    11101 tgggcgcgcg tggctggcgg ctgtttcggc gcgtgatctt tccgcttgcc atgccgggct
    11161 atctggccgg tgcggcgttg gtgttcgtca aggtgttcga cgacctgggc acaccgctgg
    11221 tgatgggtgt gaccaacatg ctggcgccgc aggcctacct gcgcatcacc tcggtggggg
    11281 tggacgaccc gctgggctac gtgatcagcg tgatcatgat cgcgttttcc atcctggcgc
    11341 tgtggctggc cgcgcgcgtg atgaagggac gcgactacgc cacgctgcaa aagggcggcg
    11401 gcgcgctgca caagcggcgc ctcacggcct gggaagcggt gctggcctat ggctggatcg
    11461 cgctggtgct gctggtggtg ctggcgccgc acgcaggcat tttgctgatg tcgttcgccc
    11521 aggtgtggag cttttcggtg ctgcccgaca gctacacgct ggcgcactac gccaccgtgt
    11581 tcagcgatgc cagcggcatg atcggcaaca ccctgctgta ctgcctgctg gcggccgggc
    11641 tggatgtggt gatcggcacc gccatcgcct acctgattct gcgcacgcga ctgccggctc
    11701 ggcagtggct ggactggctg gcttcagccg cgctggccat tcccggtctg gtgctggcga
    11761 tcggctattt gcggctgttc aggggcatgc acgttccgtt cagtgaccaa ctggtgatcc
    11821 acagctgggt gctgatcatg ctggcctacg ccgtgcggcg cctgccgtat gcgctgcgct
    11881 cgtgcatggc ggcgctgcag caggtgcacg tgtcgctgga agaagcagcg cagagcctgg
    11941 gcgcctcgcg cctgtcgacc attcggcgcg tggtggtgcc gctgatggcg ggcggcatcc
    12001 tggccggctt cgtcaccagc ttcatcaccg cggcggtgga gctgtcggcc acgctgctgc
    12061 tggtgtccag cgaaagccag gcgccgatga gcctgggcat ctacctctac atgcagagca
    12121 tcgccggtcg tgggccaggc gcggccctgg gtgtgctggc cgtgctggtg gttggcctgg
    12181 gcacgtattt ttcgcaccgt ttcgtggagc gctcgcgcgt caccaccagc gcatcgacca
    12241 aggcatgagc atgaacaagg cctccctcga atgccggcac atcagcctga gctatggcag
    12301 cacaccggtg ctgcgcgaca tcaacctgaa gatcgagccg ggcgagttct ttgccctgct
    12361 cgggccttcg ggctccggca agtccaccct gctgcgcctg atcgccggct tcaaccagca
    12421 ccagagcggc gaactgctga tcgacggcca ggacatcagc ggcgtgccgc cgcacgcgcg
    12481 ccacatcggc atggtgtttc agaactacgc gctgtggccg cacatgacgg tgtgggacaa
    12541 cgtggccttc ggcctggtgg agcggcgcga aagccgcgat gcgatccgcc gcaaggtcgg
    12601 cgcggcgctg gagctggtgg ggctggccga ctacgccaag cgccgtccgg gccagttgtc
    12661 gggcggccag cagcagcgcg tggcgctggc gcgcaccatc gtgatcgagc ccaagctgct
    12721 gctgctggac gagccgctgt ccaacctgga caagaacctg cgcgtgcaga tgcgcgagga
    12781 gctcaagaac ctgcagcgga cgctggggct gaccaccatc ttcgtcaccc acgatcagga
    12841 agaggccatg accacggccg accgcatggc ggtgctggat cagggcgtgc tgcagcaggt
    12901 gggcacggcc gccgggctgt acgactatcc ggacaaccgt ttcgtcgccg gcttcgtggg
    12961 cacggccaac ctgctggagg gcgaactgaa gatggcgccc gacggggcac tgcagtttca
    13021 cgcgcgcggc ctggggccgc tgacggtggc acggcccgac gcagcgccca cgctgggcgc
    13081 cgccacgctg gcgctgcggc cgcaccaggt cagcatcgcc gacccgcagc aggcgggcga
    13141 cgccgcacgg ctgtggctcg acgggcaggt ggagagcgcc gagttcctgg gcgagttctc
    13201 gcgctaccgc gtgcgcgtgg gcgagctggc gctgctgagc gaccagcccc actacgccgg
    13261 cgtgccgctg tttcccgccg gcgcgccggt gcgcctgggg atcgacccgc gccagctgcg
    13321 tgtgctgacg tcctgacgcg gtggcccggc ggacgggcac aatcgctgcc ggcgccgtgc
    13381 cgcgccgttc atgccatggc caagtcgaag tccgcccacg tcagcgaaac cccagccacc
    13441 gcgctgctgc gcgcgcacgg cattgccttc accgagcatc cctatgagta cgtcgagcac
    13501 ggcggcgccg agcacagcgc cgaggtgttg ggtttcgacc cgttcagcgt ggtcaagacg
    13561 ctcatcatgc aggaccagga cgcgcgccct ctgatcgtgc tgatgcacgg caaccgcaag
    13621 gtcagcacca agaacctggc gcgccagatc ggcgccaaga gcgtcgagcc ttgcaagccc
    13681 gaggtcgcca accgccacag cggctatctg gtcggcggca cctcgccgtt tggcacgcgc
    13741 cggcagatgt cggtgtacat cgaggaaagc attcttgagc tgccgcgcat tgccatcaac
    13801 ggcgggcggc gcggctacct ggtggcgatc gagccgcagg tgtgcgtgcg cctgttgggc
    13861 gcccagccgg tgcggtgcgc gatcgagctg tgatgctcga ttgctggcaa aatgcggctc
    13921 gggcgcttgt ctggcaagcg caagcagcta ttgattcagg agtacccacc gcatgatcct
    13981 gcccctgctg gcgaccctgg ccgcctacct gatcggctcg ctgagcttcg ccgtcatcgt
    14041 cagccgcgcc atgggcctgc aggacccgcg cagctacggc agcggcaacc ccggcgccac
    14101 caacgtgctg cgctcgggct ccaaggcggc ggccatcctc acactgttgc ttgacgccgc
    14161 caagggctgg ctgccggtgg tgctggtgag ggcgttcggt gccgatctcg gcctgggcgc
    14221 gggcacgcag gcgctggtgg gcctggcggc cttcctgggg cacctgtacc cggtgttctt
    14281 ccgcttccag ggtggcaagg gggtggccac ggcggccggc gtgctgttgg cctttgcgcc
    14341 ctggctgggc gtggccacgc tggtcacctg gctcatcatc gccgtgttct tccgctactc
    14401 ctcgctggcc tcgatcgtgg ccgcgctgtt tgcgccggcc tactacctgc tgggcgacgg
    14461 cgtcgcctgg accgcctcgg gctacaagac gctggccctg atggtcatgg gtgtgctgtt
    14521 gatctggcgc caccgcgaga acatccgccg cctgctggcg ggcaccgaat cgaaactggg
    14581 gagcaagaag aaatgagcgc catccagcga cacggggtgg gcgcgcgcct gtccgaggcg
    14641 gcggtcttca acggcgtggt ctacctggcc ggcatggtgc ccgaaggcgg tgcccgcgac
    14701 atccgtgccc agacggccga cgtgctggcc cagatcgacg cccagctggc cgcctgcggc
    14761 agcgaccgga cgcgcatcct gcgggcacag atctttctgg ccgacatcgc cgacatcgct
    14821 gccatgaacg aggtctggga cgcctgggtg gtgcccggcc atgcgccgcc gcgcgccacg
    14881 gtgcaggccg ctctggccga cccggcctgg cgcatcgagg tggtggtgac ggccgcccag
    14941 gcctgaccgt tactatcgaa acaatagctg ctggcgcatg ctgagcaagc gctggaggcc
    15001 gcactagcgt ttgaccagca gcatcgtggc gccgtcgcgc cgcatgttga agcggttcag
    15061 aaagctgttg cccagcagca ccgccggcat cgccgccggg gtgacgacgg cgtccacctc
    15121 gtaggccgtg acgtcgccga tgcgcaggct ggccagcttg agcgcccagc cgggcgcgtt
    15181 gccgttggcg gtgcgcaggg tcacgctgtg gccggcgcgg tagttcaggc ccagccggtc
    15241 ggcctcggcc tgaccgatgg ccaccaccga tgcgccggtg tccaccatga actgcgtggg
    15301 ccgctggttg atgctgccgg cgacaaagaa atgcccgcgc gcatccgccg tcagcgccac
    15361 ccggtcgctg ccggcgggcg cgggtgcgcc gcccacgctg gccggcgatt cgcccacgcg
    15421 cagggtcagg cgctggccac cgacgcgcac caccgctcga tcgtcgccgg tcgacagcac
    15481 ctgcacgccc aggtgcgttt cgcccggcgc cacggcgcgc ggggcctggt cgtcgacgat
    15541 cagcagcgcc cgctggccca gcagcccgtg cagcgacacg ttctgcgccc cggcccaggt
    15601 cggcagcagc agggcgccgg ccagcagggt gcgccagggg gtgcagagcg ctgccacggg
    15661 tgcgtcaatc gcggaagttg ttgaacgaca gcggcaactc cgccatgtcc ttgcgcagca
    15721 gcgccatcac ggcctgcaga tcgtcgcgct tggcgccggt cacgcgcacc gcgtcgccct
    15781 ggatggcggc ctgcaccttc agcttgcttt ccttgatcag cttctggatc ttcttgccgt
    15841 cttcggtggc gatgccgctc ttgaccttga cgagctgccg gaccttgtcg ccaccgatct
    15901 tctcgacctt gcccttgtcc agaaagcgca cgtccacgcc gcgcttggtc agcttgccgc
    15961 gcaggatgtc ttcgacctgc tgcaactgga attcggcgtc gcccagcagg gtgatttcct
    16021 tgtccttcag ctcgatggcg gcgctggtgc ccttgaagtc gaagcgcgtg ccgatttcct
    16081 tggcggcgtt gtccaccgcg ttcctgactt ccaccatgtc ggggtcgcaa accgtgtcga
    16141 aagagggcat ggccttacct ttgcatgcag ataccggatg cgacaattgt cccatgttcg
    16201 tcgagcgcaa cgtctccctg cagcccctca acagcttcgg catcgcggcg cgcgccgccc
    16261 acatggtgcg cgtgcgcagc gaggacgagg tgcgggccgt gctggccgac gccgggctga
    16321 cggcggccgg cctgttcgtg ctgggcggcg gcagcaacat cgtgctgacc ggcgatctgc
    16381 gcgcgctggt gctgaaggtc gaggtgccgg gtcgccgcgt ggtggaccag accgcgcgcc
    16441 acaccatcgt cgaggccggc gcgggcgagt cctggcacga cttcgtcggc tggacgctgg
    16501 accagggcct gcccggcctg gaaaacctgg cgctgatccc cggcaccgtg ggcgccagcc
    16561 cggtgcagaa cattggcgcc tacggggtcg agttgcagga ccgctttcac gagctcgacg
    16621 ccatcgatct ggccaccggc gagctgttca ccctgaacgc cgctcagtgc ggcttcggct
    16681 atcgcgattc ggtgttcaag cacgcgccgg cgcagccagg cgacttcggg ctggccgggc
    16741 gcgcgctgat cctgcgcgtg cgtctggcgc tgccgcgcgc ctggcggccg gtgctgggct
    16801 acctggacct ggaacgccgc atgcaggaaa ccggcatcag cgcgcccgat gcgcgccaga
    16861 tccacgactg gatcgtcgcc atccgccgcg ccaagctgcc cgacccggcg gtcatcggca
    16921 acgccggcag ctttttcaag aacccgaccg tgacgcccga gcagtgccag gacatcatcg
    16981 gtcgcgaccc caagctggtg cactacccga tgcccgacgg cagcgtcaag ctggcggcgg
    17041 ggtggctgat cgacgcctgc ggctggaagg gcaagcgcat gggcaacgcc gccgtctacg
    17101 acaagcaggc gctggtgctg gtcaaccagg gcggcagcca gcatccggcc accggcggtg
    17161 aggtgatgac cctggcgcgc gccatccaga ccagcgtgta cgagcgcttc ggcatccgcc
    17221 tggagcccga gccggtggtg gtttgagcgc caccgactgc gcccatgcgc gccatcgagc
    17281 tgttccacct gcggcgtgtg cgcgacaagc cgcgcgccct ggcgctgatc gcggcgcacg
    17341 ccaggctgtc gggcgatcag gcgctggccg tgctgcacgc agccatcggc ggcggtcggc
    17401 cgcgcctgtg tctgtcggac gatgaggcgg cgcgcgcctg catcgtggcg ctggcgcccg
    17461 ccgggttcgt ggcgcgtttc gcccccggcg ccgacttcga cctcgcccag catgcccagc
    17521 aggccctgat ggcggcgctg cccgcctgcg cccccgacct ggccgcacag gctggcgccc
    17581 ggctgctgca cgacgactgg cccgaggcgc tggcgctggc cctgcagcac ctgcgcatgc
    17641 accgtccggc gcagcacccg ggtcgacggc ggctggagca ggccgccatc gacaccggcc
    17701 tggtgcgcgg ggtgccgggg cgcacctgag cggcgcacct gggcggcggg gcggctgact
    17761 atcatcgcgc catcccgacc ctcccagatt gccccatgcg tttttccacc gtgctggccg
    17821 tgctgttgat cggcgcgctg ctgtacctcg tcatggccct gatccgtgcg gtggccatcg
    17881 gccgcatgga gggcatgcag gacgaggcac tggcctatcg ccgcaaggcc tggcgcgcgg
    17941 ccgtctttgc tgccgtggtg ctgctg
//
LOCUS       DAOQTZ010000055        17937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3592_length_17937_cov_13.535622, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000055 DAOQTZ010000000
VERSION     DAOQTZ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17937
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_17937_cov_13.535622"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PKN51_08010"
     CDS             <1..757
                     /locus_tag="PKN51_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT85082.1"
                     /translation="AERDDMVVLTLMQSGELVPEAFSRKGAGALSLCAPREMGAFLWG
                     QDARQAFLTLPRSDVVAALGHEPHTALLTAHCALAPALASQMNHVALLLGQSGRVVAE
                     EYAGLLEATRALALLALRNLGRQGAVVDLPDRTESLHAGRRAAALRFMELHAHRHELD
                     AAAIARGAGCSRTRLYEAFAAQDQTVMGALRELRLQRARALLEQGARLHVGALSWRCG
                     FADQSAFSKLFRARFGLTPTEWHVRAAVRGAVP"
     gene            754..1752
                     /locus_tag="PKN51_08015"
     CDS             754..1752
                     /locus_tag="PKN51_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT85083.1"
                     /translation="MTTSPAPCVREHWSSAEVDPAIRLDYWRDVAHNWVDVQPLSPGD
                     QLEASWSLLRGESSFFGTKRSTAYEMRTHARHVPPGEDMVVLSLLEAGEMRLNAAPGE
                     HQRVTAGMLGMYAPRQDACYRWGEGARQTYLALPRSVVVGALGREPGNLLLAPQQCAL
                     APVLASQLTHLGLLARQPARVGAEEYAGLLNATCALALLTLRNLGRQGDAGDLPDVTE
                     SLHAGRRAAAFRFMESQAHRHDLDAGAIARGAGCSRTRLYEAFAEQDRSVMDTLREIR
                     LQRARTLIERSVRLHVGLVAWRCGFADQSGFSKLFKARFGITPSEWHRWAQARQSG"
     gene            complement(1863..3059)
                     /locus_tag="PKN51_08020"
     CDS             complement(1863..3059)
                     /locus_tag="PKN51_08020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HNT85084.1"
                     /translation="MSLFSSVELAPRDPILGLNEQFAADPNPNKVNLGVGVYFDENGK
                     LPLLACVQAAEKTMMATPKPRGYLPIDGIAAYDDGVKKLVFGADSEPYQSGRIATVQG
                     LGGTGALKIGADFLKHLNPAAKVLISDPSWENHRALFTQAGFAVGTYRYYDSSTRALN
                     FEGMLADLQAAAPGTIVVLHACCHNPTGYDITPAQWDQVIAAVKARGLTPFLDMAYQG
                     FADGIAEDGAVIGKFVAAGLTFFVSTSFSKSFSLYGERVGALSVLCASKDEAARVLSQ
                     LKIMIRTNYSNPPTHGGAVVATVLNDATLRAQWEQELAGMRLRIKAMRRKLVDGLKAA
                     GVKQDMGFITTQVGMFSYSGLSKEQMVRLRSEFGVYGTDTGRMCVAALNEHNIDYVCQ
                     AIAQVV"
     gene            3162..5246
                     /gene="uvrB"
                     /locus_tag="PKN51_08025"
     CDS             3162..5246
                     /gene="uvrB"
                     /locus_tag="PKN51_08025"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNT85085.1"
                     /translation="MSASVQAVQTPSPAGQFVSFPDSPFQLFQPYPPAGDQPTAIAQL
                     VEGVRDGEAFQTLLGVTGSGKTFTMANVIARLGRPAIVFAPNKTLAAQLYSEFREFFP
                     KNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSAINEHIEQMRLSATKSVLERRDTVIVA
                     TVSAIYGIGAPEDYLQMRMILKVGGKQGQREAIGQLVRMQYQRQDIDFQRGTFRVRGD
                     TIDVFPAEHSELAVRIELFDDEVEAISLFDPLTGHIQQKVPRFVIYPSSHYVTPREKT
                     LAAVETIKLELAERLKFFVAEGKLVEAQRLEQRTRFDLEMLAELGHCKGIENYTRHLS
                     GAAPGEPPATLTDYLPRDALMFLDESHQMIGQLNAMYNGDRSRKTTLVEYGFRLPSAL
                     DNRPLKFAEFERRMHQVIFVSATPADYENQHAGQVVEQVVRPTGLVDPEVEVRPATHQ
                     VDDVLQEIRIRVQQHERVLITTLTKRMAEQLTEYLTDNGVKVRYLHSDVDTVERVEII
                     RDLRLGAFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRAARNVN
                     GKAILYADRITDSMKRAMGETERRRAKQIAFNAEHGITPRGIVKQVRDLIDGVYGERG
                     ERQVLAQGRPAVQEIALSEKDLAREIKRLEKQMLEHARNLEFEQAARVRDQLQTLKQG
                     AFGAPGTDSPSG"
     gene            5409..5942
                     /gene="iscR"
                     /locus_tag="PKN51_08030"
     CDS             5409..5942
                     /gene="iscR"
                     /locus_tag="PKN51_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HNT85086.1"
                     /translation="MRLTTKGRFAVTAMIDLALRQDSGPVTLAAISQRQRISLSYLEQ
                     LFGKLRRHELVESTRGPGGGYSLGRKADDITVADIIVSVDEPLDATHCAGKQNCQGGD
                     GPCMTHDLWTALNDRMLDFLQSVTLQKLVDDQHAKGIEIEGKPATKRAISSAPVVKPI
                     RVNAPNSVFALGGLTNK"
     gene            6028..7248
                     /locus_tag="PKN51_08035"
     CDS             6028..7248
                     /locus_tag="PKN51_08035"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNT85087.1"
                     /translation="MSQTPHFPIYMDYGATTPVDPRVVDAMVPWLREHFGNPASRSHA
                     WGWEAEQAVETAREQVAALIGADPREIVWTSGATESDNLAIKGAAQFYKGKGRHLITV
                     RTEHKAVLDVMRELERQGFEVTYLEVQPDGLLDLEALRAAIRPDTILASVMLVNNEIG
                     VIQDVQAIGRLCREKGVLLHVDAAQATGKVAIDLATLPVDLMSLASHKTYGPKGIGAL
                     YVRRKPRVRLEAQMHGGGHERGMRSGTLPTHQIVGMGEAFRIAREEMARDNEKARALH
                     RRLVDGLKDIEEAYLNGHPTQRVPHNVNMSFNYVEGESLMMGIKGLAVSSGSACTSAS
                     LEPSYVLRALGRSDELAHSSLRITIGRFTTEQEIDYAIRAIRDNVARLRELSPLWEMH
                     QDGIDLSTISWAAH"
     gene            7318..7701
                     /gene="iscU"
                     /locus_tag="PKN51_08040"
     CDS             7318..7701
                     /gene="iscU"
                     /locus_tag="PKN51_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNT85088.1"
                     /translation="MAYSQQVIDHYENPRNVGSFDKGDASVGTGMVGAPACGDVMRLQ
                     IKVNPDTGVIEDARFKTYGCGSAIASSSLVTEWVKGKTLAQATELKNSQIADELALPP
                     VKIHCSILAEDAIKAAVEDYRKKHA"
     gene            7777..8100
                     /gene="iscA"
                     /locus_tag="PKN51_08045"
     CDS             7777..8100
                     /gene="iscA"
                     /locus_tag="PKN51_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNT85089.1"
                     /translation="MTVTLTEAAARHVSKYLAQRGKGVGVRLGVKTTGCSGLAYQLEY
                     VDEVAPEDALFEQHGVKLMVDPKSLAYIDGTELDYVREGLNEGFRFNNPNERDRCGCG
                     ESFRI"
     gene            8150..8665
                     /gene="hscB"
                     /locus_tag="PKN51_08050"
     CDS             8150..8665
                     /gene="hscB"
                     /locus_tag="PKN51_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNT85090.1"
                     /translation="MQLHSTDFELFDLPPRFAQDGAALEARWKALQREVHPDRFAAQG
                     AAAQRVAAQWSARINEAHRRLKDPQRRAAYLCELHGVPVDAERHTAMPPEFLMQQMAL
                     REQLDEAGGAPALDAIAAQVAGLRHELLQALAQQQDVERDWSAAARSVRALMFLDKLA
                     ADVDRRRDTLA"
     gene            8704..10563
                     /gene="hscA"
                     /locus_tag="PKN51_08055"
     CDS             8704..10563
                     /gene="hscA"
                     /locus_tag="PKN51_08055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNT85091.1"
                     /translation="MALLQISEPGQSPDPHQRRIAVGIDLGTTHSLVAAVRHGVAECL
                     PDAQGRVLLPSVVRYPAEGGRQIGHAAASDVPAEAAHTIGSFKRLMGRGLGDIAGHER
                     LPYPLLDRPGMVAVGTPQGEKSPVELSAEILAILRQRAEDSLGGQLHGAVITVPAYFD
                     DAQRQATKDAARLAGIPVLRLINEPTAAAVAYGLDRAAEGVYAVFDLGGGTFDLSIMR
                     LTQGVFEVVATGGDTALGGDDYDHALADWVLARCAVQARTAADKAALARAARQAKEAL
                     SATETVAFRAHLTSGELVFDLKADDFAQTTQALTRRCLDTARRVLRDARLTPDDIQGV
                     VLVGGSTRMPVIRQAVAGLFGRAPLTDLNPDEVVALGAAIQAQQLAGHRPPGDLLLLD
                     VIPLSLGLETMGGLVERIVPRNQTIPTAMAQDFTTYRDGQTALALHVVQGERDLVQDC
                     RSLARFELRGIPPMAAGAARIRVTFSVDADGLLSVSAREQTSGAQAQIEVRPSYGLSD
                     QQIAQMLQDSFGTAEADVRARALAEARVEAERLRLATQGALDADGALLSAPERRAIEA
                     LMQTLRERAADGSDAAAITAATEALAQGTEAFAARRMNAGIRRALTGRNVHTL"
     gene            10567..10905
                     /gene="fdx"
                     /locus_tag="PKN51_08060"
     CDS             10567..10905
                     /gene="fdx"
                     /locus_tag="PKN51_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HNT85092.1"
                     /translation="MPTIQVLPHPEHCPDGARIQAQVGDSICEALLAHGIEIEHACDM
                     ACACTTCHVIVRQGYDSLASPEEEEEDLLDRAWGLEPQSRLSCQAVVARADLTVEIPR
                     YSINHAREKR"
     gene            10943..11656
                     /gene="dnaQ"
                     /locus_tag="PKN51_08065"
     CDS             10943..11656
                     /gene="dnaQ"
                     /locus_tag="PKN51_08065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNT85093.1"
                     /translation="MTRQIVLDTETTGLSAESGDRIIEIGCVELVGRKLTGNHLHFYL
                     NPERDSHEDALKVHGISNEFLRDKPRFAEVADEILRYVEGTELIIHNAAFDLGFLDKE
                     LERLGRPPLRSFVAGVTDTLAMAKQLYPGKRNSLDALCERLGVDNSGRTLHGALLDAE
                     LLADVYISLTRGQEALLIDLGEVGAAGGDAPRIDLTGLELPVLTANAQELAAHEDVLA
                     QIDKASGGKTVWRRVETGL"
     gene            11682..11756
                     /locus_tag="PKN51_08070"
     tRNA            11682..11756
                     /locus_tag="PKN51_08070"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11714..11716,aa:Val,seq:cac)
     gene            11850..12449
                     /locus_tag="PKN51_08075"
     CDS             11850..12449
                     /locus_tag="PKN51_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNT85094.1"
                     /translation="MLLDADDCQLVLIDYQARLMPAIHDAAAVLANARRLAQMARWLG
                     VPVWATEQNPDKLGGTDAALVPLCDRIVPKMAFGGADALLPLLTPPPAAPRGNARSLP
                     RHLQKAAPPAPERGTVVIAGCETHVCLLQSALLLLEADWDVWVVQDACGSRTERNRDA
                     AYDRLAAAGCELVTTEMVGFEWLRDCQHPQFKAWQGLIK"
     gene            12551..12973
                     /locus_tag="PKN51_08080"
     CDS             12551..12973
                     /locus_tag="PKN51_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85095.1"
                     /translation="MSSSSKQLRLSRRSSELAQAVPQVMARRVGRMMQAGPVPSMEDQ
                     QEFIRMGMEKLEAFGEAWWAMVLQGSQVQQQFALWWMQTWWKVALGGWMHPPTLSSLS
                     NGASRNLMNGWLDVTLCGVAPVHRRAVANARRLNGSGA"
     gene            13039..15024
                     /locus_tag="PKN51_08085"
     CDS             13039..15024
                     /locus_tag="PKN51_08085"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HNT85096.1"
                     /translation="MTGGRRPGAWRAWRCCPRLATRAEETSAMTSYADFHRRSIEDRD
                     AFWAEQARLIDWQTPPQTICDYSQPPFARWFVGGRTNLCHNAVDRHLKDRADQPALIW
                     VSTETDQERVYSFRDLHAEVQRMAAALRELGVKKGDRVLIYMPMIAEAAFAMLACTRI
                     GALHSVVFGGFASGSLASRIEDAEPVVVVSADAGSRGGKAIAYKPLLDEAIELSRHKP
                     SSVLLVDRGLVPMNLVAGRDQLWATLRERHLNAQVPCEWVDSTHPSYTLYTSGTTGKP
                     KGVQRDTGGYAVALAASMKHIFCGNPGETYFSTSDIGWVVGHSYIIYGPLIHGMATLM
                     YEGLPTRPDGGIWWQLVEKYKVTVMFSAPTAIRVLKKQDPAHLTRHDLSSLRSLFLAG
                     EPLDEPTAQWISGALKDKPIIDNYWQTETGWPILTICNGVERAPSKFGSPGKAVYGYD
                     IKLLDDQTGQELTAPNQKGVIAIEGPLPPGCMQTVWRDDARFVSTYWSSVPNRMVYST
                     FDWGIRDEDGYFFILGRTDDVINVAGHRLGTREIEEAIASHPNVAEVAVVGVADQLKG
                     QVAMAFAVLKDASILGDQAALLKLEGEVMKIVDGNLGAVARPARVRFVHVLPKTRSGK
                     VLRRAVQAVCEGRDPGDLTTMEDPAALQQIRDMVTPA"
     gene            15204..>17937
                     /locus_tag="PKN51_08090"
     CDS             15204..>17937
                     /locus_tag="PKN51_08090"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNT85097.1"
                     /translation="MSETKSVYQTYQGNTYLFGGNAPYVEEMYENYLANPGSVPDSWR
                     SYFDALQHVPAADGSEARDVPHLPVINAFAERAKAGGTRVVVASADAELGRKRTAVQQ
                     LIAAYRNVGARWADLDPLKRAERPAIPELEPAFYGFTDADQETVFDTSNTFFGKDKMP
                     LRELLNALRETYCGTVGAEYMYATDQNQKRWWQSKLESIRTKPVFDVDKKKRILDRLT
                     AAEGLERYLHTKFVGQKRFSLEGGESFIAAMDELIQAAGAKGVQEIVIGMAHRGRLNV
                     LVNTLGKMPKELFAEFEHTAPEDLPAGDVKYHQGFSSDVSTAGGPVHLSLAFNPSHLE
                     IVNPVVEGSVRSRMDRRGDPKGKQVLPVLVHGDAAFAGQGVNQETLALSETRGYSTGG
                     TVHIIINNQIGFTTSDPRDLRSTLYCTDIVKMIESPVLHVNGDDPESVCLAMQLALEY
                     RMEFSKDVVVDIICYRKLGHNEQDTPMLTQPLMYKKIAQHPGTRKLYADKLAAQGLGQ
                     DLGDDMVRAYRAAMDAGKHTIDPVLTNFKSKYAVDWSPFLGKTWTDAADTAIPLTEWK
                     RLAERITTIPDSVTPHALVRKVYDDRAAMGRGETNVDWGMGEHMAFASLVASGYPVRL
                     SGEDSGRGTFTHRHAVIHDQKREKWDEGTYVPLQNVAEHQAPFTVIDSILSEEAVLAY
                     EYGYASNDPNTLVIWEAQFGDFANGAQVVIDQFIASGEVKWGRVNGITLMLPHGYEGQ
                     GPEHSSARLERFMQLAADANMQIVQPTTASQIFHVLRRQMVRNLRKPLVIFTPKSLLR
                     NKDATSPLSEFTKGGFQTVLGERDEAIVKNAAKVKRVIACSGKVYYDLVKKRSEAERS
                     DVAIVRVEQLYPFPHKAFAAELKKYPNATDIVWCQDEPQNQGAWFFIQHNIHENM"
BASE COUNT         3073 a         6154 c         6055 g         2655 t
ORIGIN      
        1 cgccgaacgc gatgacatgg tggtgctgac gctgatgcag tcgggcgaac tggtacccga
       61 agccttttcc cgcaagggag caggcgccct gagcctgtgc gcgccgcgcg agatgggcgc
      121 gtttctctgg gggcaggatg cgcgtcaggc ttttctgacg ttgccgcgca gcgatgtggt
      181 cgccgcgctc gggcacgagc cacacaccgc gctgctgacg gcgcactgcg cgctcgctcc
      241 tgcgctggcc agccagatga accacgtggc cttgctactg gggcagagcg gccgggtggt
      301 tgccgaggaa tacgccggcc tgctggaggc cacgcgcgcg ctggcgctgc tggcgctgcg
      361 caatctgggt cgccagggcg ccgtcgtgga cctgcccgac cggaccgaaa gcctgcacgc
      421 cggccgccgt gccgcggcgc tgcgtttcat ggaattgcat gctcaccgac atgaactgga
      481 cgctgccgcc atcgcgcgcg gcgcgggctg ctcgcgcaca cgcctgtatg aagcgtttgc
      541 cgcgcaggac cagactgtga tgggggcgct gcgcgagctg cgcctgcaac gcgcccgggc
      601 gctgctggag cagggtgcgc ggctgcacgt gggggcgctg tcctggcgct gcggcttcgc
      661 tgaccagtcg gccttcagca agctgtttcg cgcccgcttc ggcctgacgc cgaccgaatg
      721 gcacgtgcgc gcagcggtca ggggcgcggt gccgtgacca ccagccccgc gccttgcgtg
      781 cgggagcact ggtcttccgc cgaggtcgat ccggcgatcc ggctggacta ttggcgcgac
      841 gtggcgcaca actgggtgga tgtgcagccg ctgtcgccgg gcgaccagct ggaggcgtcg
      901 tggtcgctgc tgcgcggcga gagcagcttt ttcggcacca agcgatccac cgcctacgag
      961 atgcgcaccc atgcgcgcca tgttccaccg ggcgaggata tggtggtgct gtcgctgctg
     1021 gaggccggtg agatgcggct gaacgcggcg ccgggcgagc accagcgcgt gacggcgggc
     1081 atgctgggca tgtacgcgcc gcgccaggat gcgtgttacc gctggggtga gggcgcgcgc
     1141 cagacctatc tggcgctgcc gcgcagtgtg gtggtgggcg cgctgggccg tgagccgggc
     1201 aatctgctgc tggcgccgca gcagtgcgcg ctggcgccgg tgctggccag ccagctcacg
     1261 cacctggggc tgctggcacg ccagcccgcc cgggtgggtg cagaggaata cgcgggcctg
     1321 ctgaacgcga cgtgcgcgct ggccctgctg acgctgcgca acctggggcg ccagggcgac
     1381 gcgggcgatc tgcccgatgt gaccgaaagc ctgcacgccg gccgtcgcgc cgcggcgttt
     1441 cgcttcatgg aaagccaagc gcaccggcac gatctggatg ccggcgccat tgcgcgcggc
     1501 gcgggctgtt cgcgcacgcg gctgtacgag gcgttcgccg aacaggaccg gtcggtgatg
     1561 gacacgctgc gcgagatccg cctgcaacgc gcacgcaccc tgatcgaacg cagcgtgcgc
     1621 ctgcatgtgg gtctggtggc ctggcgctgt ggctttgccg accagtcggg gttcagcaag
     1681 ctgttcaagg cgcgtttcgg catcacgcca tcagagtggc accgctgggc gcaggccagg
     1741 cagagcggtt gaggccagag ccgcgctcga tggccctttt tgaataaaaa atggcgccag
     1801 ctggcgcctg tcttgcgcgg ccagctatca ataaataact ggcggcgccg cgtgtgcggg
     1861 gctcagacca cctgagcgat agcctggcag acgtagtcga tgttgtgctc gttcagcgcc
     1921 gccacgcaca tgcggccggt gtcggtgccg tacacgccaa actcgctcct caggcgcacc
     1981 atctgctcct tggacaggcc cgagtagctg aacatgccga cctgggtggt gatgaagccc
     2041 atgtcctgct tcacgcccgc ggccttcagg ccatcgacca gtttgcggcg catggccttg
     2101 atgcgcaggc gcatgccggc cagttcctgc tcccactggg cgcgcagggt ggcgtcgttc
     2161 agtaccgtgg ctaccactgc gccgccgtgg gtgggcgggt tgctgtagtt ggtgcggatc
     2221 atgatcttga gttggctgag cacgcgtgcg gcctcgtcct tgctggcgca cagcaccgac
     2281 agcgcgccca cgcgctcgcc gtacaggctg aagctcttgg aaaacgaggt cgagacgaag
     2341 aaagtcaggc cggcagcgac gaacttgccg atcaccgcac cgtcctcggc aatgccgtcg
     2401 gcaaagccct ggtaggccat gtcgaggaag ggcgtgaggc cgcgtgcctt gacggcggcg
     2461 atcacctggt cccactgcgc gggggtgatg tcgtagccgg tggggttgtg gcagcaggcg
     2521 tgcagcacga cgatggtgcc gggcgcggcg gcctgcaggt cggccagcat gccctcgaag
     2581 ttcagcgcgc gcgtggagct gtcgtagtag cggtaggtgc ccaccgcaaa gccggcctgg
     2641 gtgaacaggg cgcggtggtt ttcccagctg gggtcgctga tcagcacctt ggccgcgggg
     2701 ttgagatgct tgagaaagtc ggcgccgatt ttcagcgcgc cggtgccgcc caggccctgc
     2761 acggtggcga tgcggcccga ctggtagggt tcggaatcgg cgccgaacac cagtttcttg
     2821 acgccgtcgt cgtaggcggc gatgccgtcg atgggcagat agccgcgcgg cttgggcgtg
     2881 gccatcatgg tcttttcggc cgcctgcacg caggccagca gcggcagctt gccgttctcg
     2941 tcaaagtaca cgcccacgcc caggttgacc ttgttcgggt tggggtcagc ggcaaactgc
     3001 tcgttcaggc cgaggatggg gtcgcgcggg gccagttcga cggaagaaaa cagcgacatg
     3061 gaaacagtcc ttgggatgag gcgccaccgg gtggcaaagg cacgccggct gagttaggct
     3121 tgccggttcg cccgaaattt taagggccac agggttttcc aatgagtgcc agtgtgcaag
     3181 ccgtgcagac gcccagtccg gcggggcagt tcgtcagctt tcccgattcg ccgttccagc
     3241 tgtttcagcc ttatcccccc gccggtgacc agcccacggc catcgcgcag ctggtggaag
     3301 gggtgcgcga cggcgaggcc ttccagaccc tgctgggcgt gaccggctcg ggcaagacct
     3361 tcaccatggc caacgtgatc gcgcggctgg gccgcccggc catcgtgttc gcgcccaaca
     3421 agacgctggc ggcgcagctg tacagcgagt tccgcgagtt cttcccgaag aacgcggtcg
     3481 agtacttcgt cagctactac gactactacc agcccgaggc ctacgtgccg cagcgcgacc
     3541 tgttcatcga gaaggacagc gccatcaacg agcacatcga gcagatgcgc ctgtcggcca
     3601 ccaagagcgt gctcgagcgg cgcgacaccg tgatcgtggc caccgtcagc gccatctacg
     3661 gcatcggcgc gcccgaggac tacctgcaga tgcggatgat cctgaaggtg ggtggcaagc
     3721 agggccagcg cgaagctatc gggcagctgg tgcgcatgca gtaccagcgc caggacatcg
     3781 actttcagcg cggcaccttc cgggtgcgcg gcgacaccat cgacgtattc ccggccgagc
     3841 attcggagct ggcggtgcgc atcgagctgt tcgacgacga ggtcgaggcc atcagcctgt
     3901 tcgacccgct cacgggccac atccagcaga aagtgccgcg ctttgtcatc taccccagca
     3961 gccactacgt cacgccgcgc gagaagacgc tggccgcggt cgagaccatc aagctggagc
     4021 tggccgagcg gctgaaattt ttcgtcgccg agggcaagct ggtcgaggcc cagcgactgg
     4081 agcagcgcac ccggttcgat ctggagatgc tggccgagct gggccactgc aagggcatcg
     4141 agaactacac ccgccacctg tcgggcgccg cgccgggcga gccaccggcc acgctgaccg
     4201 actacctgcc gcgcgacgcc ctcatgttcc tggacgaaag ccaccagatg atcggccagc
     4261 tcaacgccat gtacaacggc gaccgctcgc gcaagaccac cctggtggaa tacggctttc
     4321 gcctgcccag cgcgctggac aaccggccgc tcaagttcgc cgagttcgag cggcgcatgc
     4381 accaggtgat ctttgtcagc gccacgccgg ccgactacga gaaccagcac gccggccagg
     4441 tggtcgagca ggtggtgcgc cccaccggcc tggtcgaccc cgaggtcgag gtgcgcccgg
     4501 ccacccatca ggtcgatgac gtgctgcagg aaatacgcat ccgcgtgcag cagcacgagc
     4561 gcgtgctcat cacgacgctc accaagcgca tggccgagca gctgaccgag tacctgaccg
     4621 acaacggcgt caaggtgcgc tacctgcaca gcgatgtgga caccgtcgag cgcgtggaga
     4681 tcatccgcga cctgcgcctg ggcgccttcg acgtgctggt cggcatcaac ctgctgcgcg
     4741 aaggcctgga cattcccgag gtttcgctgg tggcgattct ggacgccgac aaggagggct
     4801 ttctgcgcgc cgagcgcagc ctgatccaga ccatcggccg cgcggcgcgc aacgtcaacg
     4861 gcaaggccat cctgtacgcc gaccgcatca ccgattccat gaagcgggcc atgggcgaga
     4921 ccgagcgccg ccgcgccaag cagatcgcct tcaacgccga gcacggcatc accccgcgcg
     4981 gcatcgtcaa gcaggtgcgc gacctgatcg acggcgtcta cggagaacgg ggtgaacggc
     5041 aggtgctggc gcagggcagg ccggcggtgc aggagatcgc cctgtcggaa aaagacctgg
     5101 cgcgcgaaat caagcggctg gaaaagcaga tgctggagca cgcgcgcaac ctggagttcg
     5161 agcaggcggc gcgggtgcgc gaccagttgc aaaccctcaa gcagggcgcc ttcggcgctc
     5221 ccggcaccga ctcgccgagc gggtgaaccc ggcacagctg ccgtgccatg cccatggcca
     5281 aaaccccacg attcgtcggg aaatggtgta tacttgatta aatcactcgg aattgatcgc
     5341 cggtgccacg gggtaatccc gaccactggc cccgagtgcc agaccctcat cgaccgaagg
     5401 aacccacgat gcgcctcacc accaaagggc ggtttgccgt caccgccatg atcgatctgg
     5461 cgctgcgcca ggacagcggc ccggtcacgc tggcggccat cagccagcgc cagcggatat
     5521 cgctgtcgta cctggagcag ttgttcggca agctgcggcg gcacgagctg gtcgaatcca
     5581 cgcgcgggcc gggcggtggc tattcgctgg gccgcaaggc ggacgacatc acggtggccg
     5641 acatcatcgt gtcggtcgac gagccgctgg acgccaccca ctgcgctggc aagcagaact
     5701 gccagggcgg tgacgggccg tgcatgacgc acgacctgtg gacggcgctg aacgaccgca
     5761 tgctggactt cctgcagtcc gtcaccctgc agaagctggt ggacgaccag cacgccaagg
     5821 gcatcgagat cgagggcaag ccggccacca agcgggccat ctccagcgcg ccggtggtca
     5881 agccgatccg cgtcaacgcc cccaattcgg tcttcgcgct gggcggcctg accaacaaat
     5941 agcgaaacga accgctggcg ctcgcccgtc gagggccagc ggcccctccg gatccgtttc
     6001 ccgcatgcct tcgagaaccc cacggccatg agccagaccc cgcatttccc gatctacatg
     6061 gactatggcg ccaccacgcc ggtcgatccg cgcgtggtgg acgccatggt gccctggctg
     6121 cgtgagcatt tcggcaaccc ggccagccgc agccacgcct ggggctggga ggccgaacag
     6181 gccgtcgaga ccgcgcgcga gcaggtggcg gcgctgatcg gcgccgaccc gcgcgagatc
     6241 gtctggacca gcggtgccac cgagtccgac aacctggcta tcaagggcgc ggcgcagttc
     6301 tacaagggca agggcaggca cctgatcacc gtcaggaccg agcacaaggc ggtgctggac
     6361 gtgatgcgcg aactggagcg ccagggcttc gaagtcacct acctggaggt gcagcccgac
     6421 ggcctgctgg atctcgaagc cttgcgcgcc gccattcgcc ccgacaccat cctggccagc
     6481 gtgatgctgg tcaacaacga gatcggcgtg atccaggacg tgcaggccat cggccgcctg
     6541 tgccgcgaaa agggcgtgct cttgcacgtc gatgccgccc aggccaccgg caaggtggcg
     6601 atcgatctgg ccacgctgcc ggtcgacctg atgagcctgg ccagccacaa gacctacggc
     6661 cccaagggca tcggcgcgct ctacgtgcgg cgcaagccgc gcgtgcggct ggaagcgcag
     6721 atgcacggcg gcggtcacga gcggggcatg cgctcgggca cgctgcccac gcaccagatc
     6781 gtcggcatgg gcgaggcgtt ccgcatcgcc cgcgaagaaa tggcgcgcga caacgaaaag
     6841 gcccgcgccc tgcatcggcg tctggtcgat ggcctgaagg acatcgagga ggcgtacctc
     6901 aacggccacc cgacgcagcg cgtgccccac aacgtcaaca tgagcttcaa ctacgtcgag
     6961 ggcgaatcgc tcatgatggg catcaagggg ctggcggtgt ccagcggctc ggcctgcacc
     7021 tcggccagcc tggagcccag ctacgtgctg cgcgcgctgg gccgcagcga cgagctggcg
     7081 cactcctcgc tgcgcatcac catcggccgc ttcaccaccg agcaggagat cgactacgcc
     7141 atccgcgcga tccgcgacaa cgtggcgcgg ctgcgcgagc tgtcgccgct gtgggagatg
     7201 caccaggacg gcatcgacct cagcaccatc tcgtgggcgg cgcattgaat tttggatcaa
     7261 atatgccatt gcggcgcttg cagaaagcgc cggcagctat gaattcagga gtaaaccatg
     7321 gcctattccc aacaggtgat tgaccactac gaaaaccccc gcaacgtcgg cagcttcgac
     7381 aagggcgacg ccagcgtggg caccggcatg gtgggtgcgc cggcctgcgg cgacgtgatg
     7441 cgcctgcaga tcaaggtcaa cccggacacc ggcgtgatcg aggacgcgcg cttcaaaacc
     7501 tacggctgcg gctcggcgat tgcctccagc tcgctggtga ccgaatgggt caagggcaag
     7561 acgctggcgc aggccaccga gctgaagaac agtcagattg ccgacgaact ggcgctgccg
     7621 ccggtcaaaa tccattgctc gatcctggcc gaggacgcca tcaaggcggc ggtcgaggac
     7681 taccgcaaga agcacgcctg aaaccggggt gagcgttcgg cgtgcgcctg gcacgcctga
     7741 cgcaagcctt ccacctgaac ctgcaaactc caacgtatga ccgtcaccct gaccgaagcc
     7801 gcggcccggc atgtcagcaa atacctcgcc caacgcggca agggcgtggg cgtgcgcctg
     7861 ggcgtcaaga ccaccggctg ctccggcctg gcctatcagc tcgaatacgt cgatgaagtg
     7921 gcgcccgaag acgcgctgtt cgagcagcac ggcgtcaagc tcatggtcga tcccaagagc
     7981 ctggcctaca tcgatggcac cgaactcgac tacgtgcgcg aggggttgaa cgaaggcttt
     8041 cgctttaaca accccaacga gcgcgaccgc tgcggctgcg gggaatcgtt ccgcatctga
     8101 cggtcttcgc gggacgctgc gcgcccgccc ggccgtgctc gcttcgatga tccagctgca
     8161 ttccaccgac ttcgagctgt tcgacctgcc gccccgcttc gcgcaggacg gcgcggcgct
     8221 ggaggcgcgc tggaaggcgc tgcagcgcga ggtgcatccg gaccgctttg ccgcccaggg
     8281 cgcggccgcc cagcgcgtgg cggcgcagtg gtcggcgcgc atcaacgagg cccaccggcg
     8341 gctgaaggac ccgcagcgcc gcgccgccta cctgtgcgaa ctgcacggcg tgccggtcga
     8401 cgccgagcgc cacaccgcca tgccgccgga gttcctgatg cagcagatgg cgctgcgcga
     8461 gcagctcgac gaggccggcg gcgcgccggc gctggacgcc atcgcggcgc aggtggccgg
     8521 gctgcgccac gagctgctgc aggctctggc gcagcaacag gacgtggagc gcgactggag
     8581 cgccgccgcc cgatcggtgc gtgcgctgat gtttctggac aagctggcgg ccgacgtcga
     8641 tcggcggcgt gacacactcg cctgaccccg tcgccccacc gggcgcccac cgaccttcgc
     8701 tgtatggcgc tgctgcagat ttccgaaccc ggtcaatcgc ccgacccgca ccagcggcgc
     8761 atcgccgtcg gcatcgacct gggcaccacc cattcgctgg tggccgccgt gcgccacggc
     8821 gtggccgaat gcctgcccga cgcgcagggc cgcgtgctgc tgccttcggt ggtgcgctac
     8881 cccgccgagg gcgggcgcca gatcggccat gccgcagcgt ccgacgtgcc ggccgaggcc
     8941 gcgcacacca tcggctcctt caagcgcctg atgggccgcg gcctgggcga catcgccggc
     9001 catgagcggc tgccctaccc actgctggac cgtcccggca tggtggcggt cggcacgccc
     9061 cagggcgaga aatcgccggt cgagctgagc gccgagattc tggccatcct gcgccagcgc
     9121 gccgaagaca gcctcggcgg ccagctgcac ggcgccgtca tcaccgtgcc ggcttacttt
     9181 gatgacgcgc agcgccaggc caccaaggac gctgcacggc tggccggcat cccggtgctg
     9241 cgcctcatca acgaacccac ggcggccgcc gtggcctacg gcctggatcg cgccgccgag
     9301 ggcgtgtatg cggtgttcga cctcggcggt ggcaccttcg acctgtccat catgcggctg
     9361 acgcagggcg tgttcgaagt cgtcgccacc ggcggcgaca cggccctggg cggcgacgac
     9421 tacgaccacg cgctggccga ctgggtgctg gcgcgctgcg ccgtgcaggc ccgcacggcg
     9481 gccgacaagg ccgcgctggc gcgcgccgca cgccaggcca aggaagccct gagtgctact
     9541 gaaacagtag cttttcgcgc acatctgaca agcggggaac tcgtttttga cctgaaagcc
     9601 gacgacttcg cccagaccac ccaggcgctc acgcgccgct gcctggacac tgcgcgccgt
     9661 gtgctgcgcg acgcccggct gacaccggac gacatccagg gggtggtgct ggtgggcggc
     9721 tccacccgca tgccggtgat ccggcaggcg gtggccgggt tgttcggccg cgcgccgctg
     9781 accgacctca accccgacga ggtggtggcc ctgggcgccg ccatccaggc ccagcagctg
     9841 gccggtcacc ggccgccggg cgatctgctg ctgctggacg tgatcccgct ctcgctgggg
     9901 ctggaaacca tgggcggcct ggtcgagcgc atcgtgccgc gcaaccagac catccccacc
     9961 gccatggcgc aggacttcac cacctaccgc gacggccaga ccgcgctggc gctgcacgtg
    10021 gtgcagggcg agcgcgacct ggtgcaggac tgccgcagcc tggcgcgctt cgagctgcgc
    10081 ggcatcccgc cgatggccgc cggcgcggcg cgcatccgcg tcaccttcag cgtcgatgcc
    10141 gatggtctgc tgtcggtcag cgcccgcgag cagaccagtg gcgcgcaggc gcagatcgag
    10201 gtcaggccca gctacggcct gtccgaccag cagatcgccc agatgctgca ggacagcttt
    10261 ggcaccgccg aagccgacgt gcgggcacgc gccctggctg aagcccgggt cgaggccgag
    10321 cgcctgcggc tggccacgca aggcgcgctg gacgccgatg gcgcactgct gagcgcgccc
    10381 gagcgccgtg ccatcgaggc gctgatgcag acgctgcgcg agcgcgccgc cgacggcagc
    10441 gacgccgccg ccatcacagc ggccaccgag gcgctggccc agggcaccga agcctttgcc
    10501 gcgcggcgca tgaacgccgg catccgcagg gcgctgaccg gcaggaacgt ccacaccctc
    10561 tgacccatgc cgaccatcca ggttcttccc catcccgagc attgccccga cggcgcgcgc
    10621 atccaggccc aggtcggcga ctcgatctgc gaggcgctgc tggcgcacgg catcgagatc
    10681 gagcacgcct gcgacatggc gtgcgcctgc accacctgcc acgtcatcgt gcgccagggt
    10741 tacgattcgc tggcgtcgcc cgaggaagaa gaggaagacc tgctcgaccg cgcctggggc
    10801 ctggagccgc agtcgcgcct gtcgtgtcag gccgtcgtcg cgcgcgccga cctgacggtg
    10861 gaaatccccc gctactcgat caaccacgcc agggaaaagc gctgaaccgc gccgcctctg
    10921 cccagcccac gccgcctgcc cgatgacccg acagatcgtt cttgacaccg aaaccaccgg
    10981 cctgtcggcc gaaagcggcg accgcatcat cgagatcggc tgcgtcgagc tggtgggccg
    11041 caagctcacc ggcaaccacc ttcatttcta cctcaacccg gagcgcgaca gccatgagga
    11101 cgcgctcaag gtgcacggca tcagcaacga attcctgcgc gacaaaccca ggttcgccga
    11161 ggtggccgac gagatcctgc gctatgtcga aggcaccgag ctgatcatcc acaacgccgc
    11221 cttcgacctg ggttttctcg acaaggagct cgagcgcctg ggtcggccgc cgctgcgcag
    11281 cttcgtcgct ggcgtcaccg acaccctggc catggccaaa cagctctacc cgggcaagcg
    11341 caacagcctg gacgcgctgt gcgagcgcct gggggtggac aactccggcc gcaccctgca
    11401 cggcgcgctg ctggacgccg aactgctggc cgacgtctac atcagcctca cgcgcgggca
    11461 ggaggcgctg ctgatcgatc tgggcgaagt cggcgccgcc ggcggcgacg ccccgcgcat
    11521 cgatctgacc gggctggagc tgccggtgct gaccgccaat gcgcaggagc tggccgcgca
    11581 cgaggacgtg ctggcgcaga tcgacaaagc cagcggcggc aagaccgtgt ggcgacgggt
    11641 cgaaaccggt ctataatgcg cgcttcgcca aaacgcgaag cgggtgatta gctcagcggt
    11701 agagcactgc cttcacacgg caggggtcgc aggttcgaac cctgcatcac ccaccaacat
    11761 ggcaacgccc cgcagtccaa cgctacgggg cgtttttgtt tccggcgcgt cgcccgcggt
    11821 ggcgccgcgg ccagccaagg agcctgtgca tgctgctcga cgccgacgac tgccaactgg
    11881 tgctgatcga ctatcaggcg cgcctgatgc ccgccatcca cgatgccgcg gcggtgctgg
    11941 ccaatgcgcg ccggctggcg cagatggcgc gctggctggg cgtgccggtg tgggcgaccg
    12001 agcagaaccc cgacaagctg ggcggcaccg acgcggcgct ggtgccgctg tgcgaccgca
    12061 tcgtgcccaa gatggcgttt ggcggcgccg acgcgctgct gcccctgctg acgccgccgc
    12121 cggccgcgcc gcgcggcaat gcgcgcagcc tgccgcgcca cctgcagaag gctgcgccac
    12181 ccgcgcccga gcgcggcacc gtggtcatcg ccggctgtga aacccatgtc tgcctgctgc
    12241 agagcgcgct gctgctgctg gaggccgact gggacgtctg ggtggtgcag gacgcctgcg
    12301 gctcgcgcac cgagcgcaac cgcgacgccg cctacgaccg gctcgccgcc gccggctgcg
    12361 agctggtgac caccgagatg gtcggcttcg agtggttgcg cgactgccag cacccgcagt
    12421 tcaaggcctg gcaggggctg atcaagtgag cggggaacac cggattgcgt gtcgccccgc
    12481 tttcgagtaa tttgtggctc cagcgcttca tgggcaagcg ccggcagcta tatttgttgt
    12541 agcaatcacc atgtcctcat cgtccaagca actgcgtctt tcgcgccgct ccagcgagct
    12601 ggcgcaggcc gtgccccagg tcatggcgcg gcgtgtcggc cgcatgatgc aggccgggcc
    12661 cgtgcccagc atggaggacc agcaggaatt catccgcatg ggcatggaaa agctggaagc
    12721 ctttggcgag gcctggtggg ccatggtgct gcagggctcg caggtgcagc agcagtttgc
    12781 gctgtggtgg atgcagacct ggtggaaggt cgcgctgggc ggctggatgc acccgcccac
    12841 gctgagcagc ctgtccaacg gcgccagccg caacctgatg aacggctggc tggacgtcac
    12901 cctgtgcggc gtggccccgg tgcatcggcg cgcggtggcc aatgcgcggc gcctgaacgg
    12961 ctcgggcgcc tgaggcacgc ccgtcagcct gccgcaagtc cggtttcccc ataattgacc
    13021 gtttgccgac aggcatcggt gacgggtggg cggcggccgg gcgcgtggcg cgcgtggcgg
    13081 tgctgccccc gtcttgcgac aagggccgag gagaccagcg ccatgaccag ctacgccgac
    13141 ttccatcgcc gttccatcga ggatcgcgat gccttctggg ccgaacaggc ccgtctgatc
    13201 gactggcaga cgccgccgca gaccatctgc gactacagcc agccgccgtt tgcccgctgg
    13261 ttcgtcggtg gccgcaccaa cctgtgccac aacgcggtcg accggcacct gaaggaccgc
    13321 gccgaccagc cggcgctgat ctgggtttcc accgaaaccg accaggagcg tgtctacagc
    13381 ttccgcgacc tgcacgccga agtgcagcgc atggcggccg cgctgcgcga gctgggggtg
    13441 aagaagggcg accgggtgct gatctacatg cccatgatcg ccgaggccgc cttcgccatg
    13501 ctggcctgca cgcgcatcgg cgcgctgcat tcggtggtgt ttggcggttt tgcctcaggc
    13561 tcgctggcca gccgcatcga ggacgccgaa cccgtggtcg tggtcagcgc cgacgccggc
    13621 tcgcgtggcg gcaaggcgat cgcctacaag ccgctgctgg acgaggccat cgagctgtcg
    13681 cgccacaagc catcgtcggt gctgctggtg gaccgcggtc tggtgccgat gaacctggtg
    13741 gccgggcgcg accagctgtg ggcgacgctg cgcgagcggc acctgaacgc ccaggtgccc
    13801 tgtgagtggg tcgactccac ccatccgagc tacacgctct acaccagcgg caccaccggc
    13861 aagcccaagg gcgtgcagcg cgacaccggc ggctatgccg tggcgctggc cgcttcgatg
    13921 aagcacatct tctgcggcaa cccgggcgaa acctacttct ccaccagcga catcggctgg
    13981 gtggtgggcc acagctacat catctacggc ccgctgatcc acggcatggc cacgctcatg
    14041 tacgaaggcc tgccgacgcg gcccgacggc ggcatctggt ggcagctggt cgagaaatac
    14101 aaggtcaccg tgatgttcag cgcccccacg gcgatccgtg tgctcaagaa gcaggacccg
    14161 gcgcacctga cgcgtcacga cctgtccagc ctgcgctcgc tgtttctggc gggcgaaccg
    14221 ctggacgagc cgacggcgca gtggatcagc ggcgcgctca aggacaagcc gatcatcgac
    14281 aactactggc agaccgagac cggctggccg atcctgacca tctgcaacgg cgtcgagcgg
    14341 gcgcccagca agttcggctc accgggcaag gcggtgtacg gctacgacat caagctgctg
    14401 gacgaccaga ccggccagga gctgacggcg cccaaccaga agggtgtgat cgccatcgaa
    14461 ggcccgctgc cgcccggctg catgcagacc gtctggcgcg acgacgcgcg cttcgtcagc
    14521 acctactggt ccagtgtgcc caaccgcatg gtgtattcca ccttcgactg gggcatccgc
    14581 gacgaagacg gctatttctt catcctcggg cgcaccgacg acgtcatcaa cgtcgccggc
    14641 caccgcctgg gcacgcgcga gatcgaagaa gccatcgcca gccatcccaa cgtcgccgaa
    14701 gtggcggtgg tgggcgtggc cgaccagctc aagggccagg tcgccatggc cttcgcggtg
    14761 ctcaaggacg ccagcatcct gggcgaccaa gcggcgctgc tgaagctgga gggcgaggtg
    14821 atgaagatcg tcgacggcaa cctgggcgcg gtggcgcgcc cggcgcgcgt gcgcttcgtc
    14881 cacgtgctgc ccaagacgcg ctcgggcaag gtgctgcgcc gcgccgtgca ggcggtgtgc
    14941 gaggggcgcg atccgggcga tctgaccacc atggaagacc cggcggcgct gcagcagatc
    15001 cgggacatgg tcacgccggc gtgagtggcg cgccggcgcg gcgggccgtc acatgcccgt
    15061 cagtgttcgg tggcacaatc cgcagcttgc gtagtacacg gcccttccca gcctgtcgcg
    15121 tccgccaacc ggttcagccg tgacgcggaa ggttttccaa ccaactgaag ctccctgaag
    15181 gggggcgaaa ggtcagcgaa gcaatgagcg agacgaaatc cgtctatcaa acctaccagg
    15241 gcaacaccta tctcttcggc ggcaatgcgc cctacgtcga agagatgtac gaaaactacc
    15301 tggccaaccc gggcagcgtg ccggactcct ggcgcagcta cttcgatgcc ctgcagcatg
    15361 tgccggcggc cgacggcagc gaggcgcgcg acgtgccgca cctgccggtc atcaacgcct
    15421 ttgccgaacg cgccaaggcc ggtggcacgc gggtggtggt ggccagtgcc gacgccgaac
    15481 tgggacgcaa gcgcaccgcg gtgcagcagt tgatcgccgc ctaccgcaac gtgggcgccc
    15541 gctgggccga cctcgacccc ctgaagcgcg ccgagcgccc cgccatcccc gagctcgaac
    15601 cggccttcta cggcttcacc gacgccgatc aggaaacggt gttcgacacc agcaacacct
    15661 tcttcggcaa ggacaagatg ccgctgcgcg agctgctcaa tgctctgcgc gagacctact
    15721 gcggcaccgt gggcgccgaa tacatgtacg ccaccgacca gaaccagaag cgctggtggc
    15781 agagcaagct ggagtcgatt cgcaccaagc cggtgtttga cgtcgacaag aaaaagcgca
    15841 tcctcgatcg cctgacggcc gccgagggtc tggagcgcta cctgcacacc aagttcgtcg
    15901 gtcagaagcg cttttcgctg gagggcggcg aatccttcat tgccgccatg gacgagctga
    15961 tccaggccgc cggcgccaag ggcgtgcagg aaatcgttat cggcatggcc caccgtggcc
    16021 gcctgaacgt gctggtcaac accctgggca agatgcccaa ggagctgttc gccgagttcg
    16081 agcacaccgc gcccgaagac ctgcctgccg gcgacgtgaa ataccaccag ggcttcagct
    16141 ccgacgtgtc caccgccggt ggcccggtgc acctgtcgct ggccttcaac ccctcgcacc
    16201 tggaaatcgt caacccggtg gtcgaaggct cggtgcgttc gcgcatggac cgtcgcggcg
    16261 accccaaggg caagcaggtg ctgccggtgc tggtgcacgg tgacgccgcc ttcgccggcc
    16321 agggcgtgaa ccaggaaacg ctggcgctgt ccgagacccg tggctattcc acgggcggta
    16381 cggtgcacat catcatcaac aaccagatcg gcttcaccac cagcgacccg cgcgacctgc
    16441 gctccacgct gtactgcacc gacatcgtca agatgatcga gtcgccggtg ctgcacgtca
    16501 acggcgacga ccccgagtcg gtctgcctgg ccatgcagct ggcgctggaa taccgcatgg
    16561 agttttccaa ggacgtggtg gtggacatca tctgctaccg caagctgggc cacaacgagc
    16621 aggacacccc catgctcacc cagccgctga tgtacaaaaa gatcgcccag caccccggca
    16681 cgcgcaagct ctacgccgac aagctggccg cccagggtct gggacaggac ctgggcgacg
    16741 acatggtgcg cgcctaccgc gccgccatgg acgccggcaa gcacaccatc gatccggtgc
    16801 tgaccaactt caagagcaag tacgcggtcg actggtcgcc cttcctgggc aagacctgga
    16861 ccgacgccgc cgacaccgcc attccgctga ccgagtggaa gcgcctggcc gagcgcatca
    16921 ccaccatccc cgacagcgtt acgccgcacg cgctggtcag gaaggtctac gacgaccgcg
    16981 ccgccatggg ccggggcgag acgaacgtgg actggggcat gggcgaacac atggcgtttg
    17041 cctccctggt ggccagcggc taccccgtgc gcctgtccgg cgaggacagc ggccgcggca
    17101 ccttcacgca ccgccacgcc gtgatccacg accagaagcg cgagaagtgg gacgaaggca
    17161 cctacgtgcc gctgcagaac gtggccgagc accaggcgcc gttcaccgtc atcgactcca
    17221 tcctgtccga agaggccgtg ctggcctacg aatacggtta cgcctccaac gaccccaaca
    17281 cgctggtgat ctgggaggcg cagttcggcg acttcgccaa cggcgcgcag gtggtgatcg
    17341 accagttcat cgcctcgggt gaggtcaagt ggggccgtgt gaacggcatc acgctgatgc
    17401 tgccgcacgg ctacgagggc cagggccccg agcacagctc ggcacgcctg gagcgcttca
    17461 tgcagctggc ggccgacgcc aacatgcaga tcgtgcagcc gaccacggcg agccagatct
    17521 tccacgtgct gcgtcgccag atggtgcgca acctgcgcaa gcccctggtc atcttcacgc
    17581 ccaagagcct gctgcgcaac aaggacgcga cctcgccgct gtccgagttc accaaggggg
    17641 gcttccagac cgtgctgggc gagcgcgacg aggcgattgt caagaacgcg gccaaggtca
    17701 agcgcgtcat cgcctgctcg ggcaaggtgt actacgacct cgtgaagaag cgcagcgagg
    17761 ccgagcgcag cgacgtggcc atcgtccgcg tcgagcagct ctacccgttc ccgcacaagg
    17821 ccttcgcggc cgagctcaag aagtacccga acgccaccga catcgtgtgg tgccaggacg
    17881 agccgcagaa ccagggcgcc tggttcttca tccagcacaa catccacgag aacatgc
//
LOCUS       DAOQTZ010000056        17651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3661_length_17651_cov_12.992044, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000056 DAOQTZ010000000
VERSION     DAOQTZ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17651
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3661_length_17651_cov_12.992044"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKN51_08095"
     CDS             complement(<1..308)
                     /locus_tag="PKN51_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNT85098.1"
                     /translation="MAKIIGIDLGTTNSCVSIMEGNSTRVIENAEGARTTPSIVAYQE
                     DGEVLVGASAKRQAVTNPKNTIYAAKRLIGRKFDEKEVQKDIDLMPFKIVKADNGDAW
                     V"
     gene            complement(416..973)
                     /gene="grpE"
                     /locus_tag="PKN51_08100"
     CDS             complement(416..973)
                     /gene="grpE"
                     /locus_tag="PKN51_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNT85099.1"
                     /translation="MSDSLHSPPPFDPPMSPEELEAAAAAQEADAAAAQESALAELNS
                     LKAQVAALTDQALRAQAEAQNARRRADDEIAKIRKFAVESFAESLLPVIDSLEAGLKI
                     ESASTEQLREGTDATLRQLLSVLERNKVVEVNPAPGARFDPASQQAISMVAAAQEAGT
                     VVSVLQKGYTIAERVLRPALVTVAQ"
     gene            complement(1155..1499)
                     /locus_tag="PKN51_08105"
     CDS             complement(1155..1499)
                     /locus_tag="PKN51_08105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT85100.1"
                     /translation="MSTGLQTIDTLPGTGAEARKGQTVTVHYTGWLYQNGQQGAKFDS
                     SRDRNEPFRFALGAGMVIRGWDEGVAGMKVGGQRTLIIPPELGYGARGAGGVIPPHAT
                     LKFDVELLAVSN"
     gene            complement(1612..1920)
                     /locus_tag="PKN51_08110"
     CDS             complement(1612..1920)
                     /locus_tag="PKN51_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT85101.1"
                     /translation="MSSKENSATSQLLALLEARYAIRVLWALRDGHPQTFRLLQDSVG
                     GITPNTLNTRLKELRAANLVTHGSDGYSLTPSGADLTKRLSDLQAFATKWVAAQTKKK
                     "
     gene            complement(2073..2555)
                     /locus_tag="PKN51_08115"
     CDS             complement(2073..2555)
                     /locus_tag="PKN51_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNT85102.1"
                     /translation="MPSPTRSPAATATPTSGTEAVDRRFLQSLVGFNARMATLKVISQ
                     FIPRMAPFELRVVDFSILSLIHHNPGITSRQLCDALNLLPPNLVGKVNALQRRGLLLR
                     RPHPDDGRAQALHLTDAGLELMRRAEPTVVALENEAAAALTVNERQTLIRLLQKVYQD
                     "
     gene            complement(2699..3838)
                     /locus_tag="PKN51_08120"
     CDS             complement(2699..3838)
                     /locus_tag="PKN51_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT85103.1"
                     /translation="MKRHSFRTLVATAALAASGAALADVTIGVIAPLTGPASGLGIPV
                     GNHVKLWPKEIAGQKVNVIVMDDATDPTNGVKIAKRFVAEDKVDVIMGSVATPVAAAI
                     SQVAEESGTAQIAWSPVALPSGKDKWVFRLPQSNAVMSHAVIEHMKKQGIKSVGFLGY
                     SDAYGETWLVDFKPMAEKAGIQVGPVERFARADTSVTGQALKLASANPQAILVVASGS
                     GAAMPEMALRERNYKGTIYQTHAAATRDLMRVGGKAVEGTFVVSGPAVLAEQLPDSHP
                     SKGEAIKYVQQYEKAYGPGSRNQFAAHAYDSVIIMEKALPIALKTAKPGTPEFRAALR
                     DAFEQLGRTVLSHGVLNWTAQDHWGYAPETGVMMKVVDGQFKVEK"
     gene            complement(3885..4298)
                     /locus_tag="PKN51_08125"
     CDS             complement(3885..4298)
                     /locus_tag="PKN51_08125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT85104.1"
                     /translation="MSKTTTYELRVMFGDCDPAGIVFFPNFSKWMDASSLDFFMQCGV
                     PPWRELKESHGIIGTPLLEIHTKFMRPATYGDTLQVRTCVESWAAKTFVQKHQVFKGE
                     ELLCEGRETRAFCVHPEGGRLQAIAVPDFIRERCS"
     gene            complement(4295..>5014)
                     /locus_tag="PKN51_08130"
     CDS             complement(4295..>5014)
                     /locus_tag="PKN51_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT85105.1"
                     /translation="GLGEATARELARLGARVAVLDLNLDNARRVASDIGGLAIQTDVS
                     SGESMQAAIEQAAAAHGPARILMQIAGIGTAKRVIGKDGSAAPLEDFIRVVNVNLIGT
                     YNAARLFAAACAKLEPLEDGERGAMVFTASVAAFDGQVGQQAYSASKGGVVGMTLPMA
                     RDLAQHGIRVCTIAPGLFATPLMRTLPEPVQASLAASIPFPPRLGKPSEFAELACHIV
                     TNGHLNGEVIRLDGALRMAAR"
     assembly_gap    5015..5114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5115..6058)
                     /locus_tag="PKN51_08135"
     CDS             complement(<5115..6058)
                     /locus_tag="PKN51_08135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNT85106.1"
                     /translation="MAHKGFFNAFNDVWLLDGVRTPMVDYCGALGHVSPTDLGIKAAR
                     AALQRAGVPGGDIGSVVCGNMAPGDFDQFFLPRHIGLYAGVPVEVPAIMAQRICGTGF
                     ELLRQAGEHVQGGACEAALVVGTENMTRNPIAAFEHRTGFKLGAPVGFKDYMWEALKD
                     PAAGINMIQTAENLAKKYGITREEVDAFASASFARAVAAQASGFLAGEIVPVVSEKFA
                     LDGYKPRGIKLQGKTTELTQDTHPRPSPPEVLAKLRPVFEGGVQTGGNSSALVDAAAA
                     AVVASGAYAKAHGKQPLARIVGAAAVGVPPEIMGIGPAP"
     gene            complement(6073..7974)
                     /locus_tag="PKN51_08140"
     CDS             complement(6073..7974)
                     /locus_tag="PKN51_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HNT85107.1"
                     /translation="MSIPRAAAAAAPRYRPLRFGVTQARVRGGAGGVQYLNAEQALQP
                     FAERMSDRLRHWAQATPEATFLAQRVRAADGQLGDWRRISYAQALDGARRIGQALLAR
                     GLSAERPVLILSENSLEHALLALGCMWAGVPWCPASPAYSLISKDYGKLRHIVGTLTP
                     GLIWAEDGARYGPAMAAVAPSDCELALSRHGDAPSLQGRSATAFDTLLATEATPAVDA
                     AQAATGPDTIVKFLFTSGSTKLPKAVINTHRMWCANQQQMRQSMPVLTEEPPVLVDWL
                     PWNHTFGGNHNVGMVLYNGGALYIDEGKPTPALIQETLRNLREIAPTLYFNVPTGFEA
                     IANAMQHDDQLRRTLLSRVKMFFYAGASLAQPVWDSLFASAEREVGERIVMASGLGMT
                     ESSPFGLFVTSPDVRAGDLGLPAPGLELKLVPEGDKLEIRYRGPNITPGYWRAPETTA
                     EAFDDEGFFCTGDAVAWIDPRQPDLGLRFDGRIAEDFKLATGTFVSVGPLRGRVITGG
                     APYIQDVVITGLNRAEVGALIFPTPAVRALTPLPADAPMTDVLRHPAVLQKFHSLVVD
                     LSLTATGSANRVARLMLLAEPPSIDKGEVTDKGSINQRAVLKERDALVQALYDEAPGH
                     IVIKPDSRF"
     gene            complement(8052..8840)
                     /locus_tag="PKN51_08145"
     CDS             complement(8052..8840)
                     /locus_tag="PKN51_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNT85108.1"
                     /translation="MHVTHDLSVELLGPAQHLAVVRLTRAAKRNALSDGLVAALRDAF
                     QNLPASVRAAVLTGEGEHFCAGLDLSELKERDAGAGLHHSRSWHVALDAIQYGAVPVV
                     AALHGAVVGGGLELACAAHIRVADDTTFYALPEGSRGIFVGGGGSVRIPKLIGVARMT
                     DMMLTGRVYHAADGERLGFAQYLVPAGNAFDKALELAERIATNAPLTNYALMHALPRI
                     AEQPADHGLLTEALISAIAQSAPEAKERVRDFLAGKAGTKVKKG"
     gene            9020..9985
                     /locus_tag="PKN51_08150"
     CDS             9020..9985
                     /locus_tag="PKN51_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HNT85109.1"
                     /translation="MQRRHLLAGLAAVTLTFGPAWANGFPSRPIRIVVPNAAGGAADI
                     TARTVGEHMARTLGQPVVVENKPGAGGVVAGQAVAGAAPDGHTLLLISSGTAVSQALF
                     KSLPYDTLKAFTPVAPLASFDLVIVSKEGGAFKSLGDLIAYARQNPGKLNIGTPNIGT
                     TQNLAAELFKSAAGLDVQVVPFKATPDVIGAIRGDQIQAGLDIVPPLLTQIQAKVLRP
                     LAVTGAKPSRVLPGVPTAQEAGVKGLNAASWNGLAVPAGTPAAVIDKLNQAVNAALRD
                     PEVVKKLQTLNLDPHPGSAQDALAMLSGDIQRWNQVIDKARIERK"
     assembly_gap    9987..10086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10087..10610
                     /locus_tag="PKN51_08155"
     CDS             <10087..10610
                     /locus_tag="PKN51_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HNT85110.1"
                     /translation="GVMTSVPPYGIRVPMLLGGAHAAELAARLGGWGMDARAVSAELG
                     VASAIKMCRSVMIKGLEALVIESYTAARAYGVEEHVLPTLQETFPGIDWSAQGAYFFS
                     RVAQHGQRRAEEMRESARTVQEAGFEPLMTAAIAAKQQWVADQARAGAFKPIVKGARW
                     QDFADALLRQRQA"
     gene            10623..11567
                     /locus_tag="PKN51_08160"
     CDS             10623..11567
                     /locus_tag="PKN51_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNT85111.1"
                     /translation="MLDILAITGPIYLCVALGWLTTRAGLFARADMRVLGQFVLYIAL
                     PALLFGAVGQRPIADILDWHYMAVFALGGLATIGLGLFWARRLAGQAPLAGVVQAMGM
                     SCPNSGFVGYPINLLVLGGPTAGVILGMNMLVENLLLIPLLLALADSASGGGHWRAVL
                     RGVAVGLWRNPLVLGLAAGLAAALLGWRLPGPLERTVGLLAQSSAALSLFVIGGSLAG
                     LRLAGMGRTVARIAAGKLIAHPLLTLGALWLLAALGAPLSDPALKKGVVLATACPMFG
                     IYPILAGRFGAEGLAAAALLGTTVASFFSISALLWWLG"
     gene            complement(11769..12152)
                     /locus_tag="PKN51_08165"
     CDS             complement(11769..12152)
                     /locus_tag="PKN51_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNT85112.1"
                     /translation="MTHRILADRIRLKRAYEPASADDGARILVERLWPRGLSKQTLAL
                     TEWARDVAPSTALRQWYGHEPTRWDGFRQRYAAELRQQPAALEALRQRARAGVVTLIY
                     AARTDTLNNAVALREFLLHPERLEP"
     gene            complement(12279..13256)
                     /locus_tag="PKN51_08170"
     CDS             complement(12279..13256)
                     /locus_tag="PKN51_08170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HNT85113.1"
                     /translation="MHAWLCENPVGVDALTWQELPTPQPGKGQVLIRIQAASLNFPDL
                     LIVQNKYQFKPELPFVPGSEYAGVVEAVGEGVTELKVGQAVACLSGTGGFATHTLAPA
                     KLCMPLPPGFPPADAAAFIMIYATSHHALMDRGQLKAGETVLVLGAAGGVGMSAIQIA
                     KIAGARVIAAASTEEKLAFCRQLGADDTINYSTQDLRERLKELTGGKGPDVIYDPVGG
                     DFTEPAFRSIAWRGRHLVIGFAAGEIPALKVNLALVKGASIVGVFWGDFARREPQANA
                     AMMQELASWYAQGKIKPLIDRQMPMSELKAAYARMGSRQVQGKLVLVNG"
     gene            13434..14222
                     /gene="surE"
                     /locus_tag="PKN51_08175"
     CDS             13434..14222
                     /gene="surE"
                     /locus_tag="PKN51_08175"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNT85114.1"
                     /translation="MRILLCNDDGYQAAGIVAFHRALAALPGVQVEVVAPEHNNSAQS
                     NALTLRAPLYVNQAANGFRYVNGTPADCVHIALTGLLDYRPDLVVSGINNGANMGDDT
                     IYSGTVGAAMEGYLFGIPALAFSLVDKGWAHIDAAAVRAAELVARFGAHPPGGGKPWL
                     LNVNIPNLPLAQIRPPKVCRLGRRHAAQSAITMTSPHGETMYWIGGAGSAADAAEGTD
                     FHATAEGHVAITPLKVDLTDHDALSYWAQGLGHLLAAGAAGGAG"
     gene            14222..15034
                     /locus_tag="PKN51_08180"
     CDS             14222..15034
                     /locus_tag="PKN51_08180"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNT85115.1"
                     /translation="MATPPRPGFPARIDLGTGGKSAARPPARPAGVAGASANPPLTSA
                     TAPQAASMARDPIPARARMVQLLAAEGIRDPQVLRAMGAVDRHHFVDGALVPQAYEDT
                     ALPIGLGQTISKPSVVARMLELLMQGAVRGPDGRLGRVLDIGTGCGYQAVVLAQLASE
                     VYSIERLRDLHDKARANLRPLRLATVHLILGDGMAGFARGAPYAGIVAAAGGEAVPEA
                     WIDQLAWGGRIVAPVATAAGQQALIVIDKSRQGVQQTVLEAVNFVPLKSGIA"
     gene            15067..15975
                     /locus_tag="PKN51_08185"
     CDS             15067..15975
                     /locus_tag="PKN51_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT85116.1"
                     /translation="MYLTGRRILLAATLLGGVVLAGCSGTPLTPAPVEDRDTRGTPSR
                     VDPATLPGFENAGKPGYYTVRPGDTIMRIATEVNHPWRDIARWNNLENPNVIEVGQVL
                     RVVPPQGATAGAATPITAVPLPAPPVAASGAKPAPQPVPVATQTTPGVSAPAAPTAAP
                     ASAPTPPAPAPAAAPPSAGADNVDFIWPASGSVVAGFDEAKNKGLGIGGKAGDPVLAA
                     ADGRVVYAGAGLRGYGNLIILKHNNTFLTAYAHNQKLLVKEDQDVRKGQKIAEMGSSD
                     TDRVKLHFEIRRSGKPVDPARYLPAR"
     gene            16102..17541
                     /gene="rlmD"
                     /locus_tag="PKN51_08190"
     CDS             16102..17541
                     /gene="rlmD"
                     /locus_tag="PKN51_08190"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNT85117.1"
                     /translation="MNLEPDHSRDAALPEGWLQVDSIDLEAQGIARRPDGKVVFIDGA
                     LAGELVSVSVHRRKNQWEAATLTELHRASSLRVRPRCPHFGLHQGACGGCKMQHLDAS
                     AQVAVKQRALEDQLWHLGKVRPALILRPIEGPAWGYRWRARLSVRYVAKRGEVLVGFH
                     ERKSRYVADMRECPVLPAHVSALLLPLRALIHGMQARDSCPQIELACGDEVTALVLRH
                     LEPLSAADRQRLSEFGRQHGVQWWLQPKGPDSVQRLEPGGPELAYALAEFGLRMPFRP
                     TDFTQVNPHINRVLVGRALRLLQAQRHERVIDWFCGLGNFTLPIATTAGQVLGVEGSA
                     ALVDRARQNHEQNRAAARTGSALAATEFVARNLFDMSAAQLVQDGSADRWLVDPPREG
                     AFALVKALAAIHQGRLGAPDAEPLPAGADGWRPPRRIVYVSCNPATLARDAGLLVHQA
                     GYRCSAAGVVNMFPHTAHVESMAVFDLQD"
BASE COUNT         2545 a         5808 c         6283 g         2815 t
ORIGIN      
        1 acccaggcgt cgccgttgtc ggccttgacg atcttgaagg gcatcaggtc gatgtccttc
       61 tgcacttcct tttcgtcgaa cttgcggccg atcaggcgct tggcggcgta gatggtgttc
      121 ttggggttgg tgacggcctg gcgcttggcc gaggcgccca ccagcacctc accgtcttcc
      181 tggtaggcga cgatggacgg cgtggtgcgc gcgccttcgg cgttctcgat cacgcgggtc
      241 gagttgcctt ccatgatgga cacgcagctg ttggtggtgc ccaggtcgat gccgatgatt
      301 tttgccatga tggttctccg cgaggggtag atgaatgagg ttgtggttca ggtggagggc
      361 cggcgcgcga aattcaagca gcgtcgccag gcccgcgcat cagacggtgg gtcgattact
      421 gggccacggt gaccagggct ggccgaagca cgcgttcggc gatggtgtag cccttttgca
      481 gcacgctgac gacggtgccg gcctcctgag cggccgccac catgctgatg gcctgctggc
      541 tggcagggtc gaaacgggcg ccgggtgcgg ggttgacttc gaccaccttg ttgcgctcca
      601 gcacgctgag cagttgccgc agggtggcgt cggtgccttc acgcagctgt tcggtgctgg
      661 cgctttcgat cttcaggcca gcctccaggc tgtcgatcac cggcagcagg ctttcggcga
      721 aggactcgac ggcgaacttg cggatcttgg cgatctcatc gtcggcgcgc cggcgggcgt
      781 tctgggcttc ggcctgggca cgcagcgcct ggtcggtgag ggcggcgacc tgcgctttca
      841 ggctgttcag ctcggccagt gcgctttcct gggcggcggc ggcatcggcc tcctgggcgg
      901 cggcggccgc ctccagctct tcggggctca tgggagggtc gaatgggggt ggggaatgga
      961 gcgagtcgga catgcaggct ggtgaaaaaa ggcgaacaga cggaacaaac ccccctcaga
     1021 tggccccgga acgcgggttt tcaagggcag ggcggcccga aaaccggcga tctggggagc
     1081 gccacttggc tgccatgggt tccagaacgc aaaccgccgc ccgcaggcgg cgttgcaggg
     1141 ggggtggccc aggctcagtt cgagacggcg agcaactcga cgtcgaactt cagcgtcgca
     1201 tgaggcggaa tcacgccgcc ggcgccgcgg gcgccgtagc ccagttcggg cgggatgatc
     1261 agggtgcgtt ggccgccgac cttcatgccc gccacgcctt cgtcccagcc acggatcacc
     1321 atgccggcac cgagcgcgaa gcggaacggt tcgttgcggt cgcggctgga gtcgaacttg
     1381 gcaccctgct gcccgttctg gtagagccag ccggtgtagt gcacggtgac ggtctggccc
     1441 ttgcgggcct cggcgccggt gccgggcagg gtgtcgatgg tttgcaatcc ggtgctcatg
     1501 aaatactata attttgatag cttgtggcgc ttgccagata agcgctgggg ccggacttgg
     1561 ttagaagaac cgggttggcc ggaggaggcc ccgagcgcgc cgccgcgcgc ctcacttctt
     1621 cttggtctgt gccgccaccc acttggtggc gaaggcctgc aggtcggaca ggcgcttggt
     1681 cagatcggcg ccgctggggg tgaggctgta gccgtcgctg ccgtgggtga ccaggttggc
     1741 cgcgcgcagc tccttcaggc gggtgttgag ggtgttgggc gtgatgccgc cgacgctgtc
     1801 ttgcagcagg cggaaggttt gcggatggcc gtcgcgcagg gcccacagaa cacgaatggc
     1861 gtatcgcgct tcaagcagcg ccagcagctg actggtggcg gaattttctt tggaactcat
     1921 gtcggggttc tcctcgggtt cgctcggtgc gtgaggggac tatatcgcaa atctgtagca
     1981 cgctagcagt ttcatagctc ccggcactgc cgggccgtgg atgtggcgct acaaatagtg
     2041 gaatcgaccg aagcgtcggc ccgggaaaac ccttagtctt gatagacctt ctgcagcagc
     2101 cggatcaggg tctgcctttc gttcaccgtc agggcggcgg cggcttcgtt ctccagcgcc
     2161 accacggtgg gttcggcgcg ccgcatcagc tcaaggcctg cgtcggtcag gtgcaaggcc
     2221 tgggcgcggc cgtcgtccgg gtggggtcgg cgcagcaaca ggccgcgtcg ttgcagggcg
     2281 ttgaccttgc ccaccaggtt ggggggcagc agattgagcg cgtcgcacag ctggcgcgag
     2341 gtgatgccgg ggttgtggtg gatcagcgac agtatcgaaa aatcgaccac ccgcagctca
     2401 aagggcgcca tgcgcggaat gaactggctg atcaccttga gcgtcgccat gcgggcgttg
     2461 aagccgacca gcgactgcag aaagcgccga tccaccgcct cggtgccgga ggtgggcgtt
     2521 gcggtggcgg cgggggagcg ggtgggcgaa ggcacggggc gcgggcggct gggggcaggg
     2581 cggatgatcg cacagcgctt gcgggcggcg cacacgacgg cgccctccgg caagcgctgc
     2641 aacaagccct ggcgctgcgg tctgcccgcg gccccgtggg gtgggtgccg cgcggtcgtt
     2701 acttttcgac cttgaactga ccgtccacca ccttcatcat cacgccggtt tcaggcgcgt
     2761 agccccagtg gtcctgcgcg gtccagttga gcacgccgtg cgagagcacg gtgcggccca
     2821 gctgctcgaa ggcgtcgcgc agcgcggcgc ggaattcggg cgtgccgggc ttggcggtct
     2881 tcagggcgat cggcagcgcc ttctccatga tgatgaccga gtcgtaggcg tgggcggcaa
     2941 actggttgcg cgagccggga ccataggcct tttcgtactg ctgcacgtac ttgatggcct
     3001 cgcccttgga cgggtgagag tcgggcaact gttcggccag caccgccggg ccggagacca
     3061 cgaaggtgcc ttccaccgcc ttgccgccca cgcgcatcag gtcacgtgtg gcggcggcat
     3121 gggtctggta gatcgtgccc ttgtagttgc gctcacgcag cgccatttcg ggcatggcgg
     3181 cgccgctgcc cgaggccacc accaggatgg cctgggggtt ggccgacgcg agcttgagcg
     3241 cctgcccggt cacgctggtg tcggcgcggg caaagcgctc caccgggccg acctggatgc
     3301 cggctttttc ggccatgggc ttgaagtcga ccagccaggt ttcgccatag gcgtcgctgt
     3361 agcccagaaa gcccaccgac ttgatgccct gcttcttcat gtgctcgatc accgcatggc
     3421 tcatcacggc gttggactgc ggcaggcgga acacccattt gtccttgccc gaaggcagcg
     3481 ccaccggcga ccaggcgatc tgcgcggtgc ccgattcctc ggccacctga ctgatggccg
     3541 cggccacggg cgtggcgacc gagcccatga tcacatccac cttgtcttcg gccacgaagc
     3601 gcttggcgat cttgacgcca ttggtcgggt cggtggcgtc gtccatcacg atcacgttga
     3661 ccttctggcc ggcgatctcc ttgggccaga gcttgacgtg gttgcccacc ggaatgccca
     3721 ggcccgaagc cggcccggtg agcggggcga tcaccccgat ggtgacatcg gccagcgccg
     3781 cgccgctggc ggccagggcg gcggtggcca ccagggttct gaaggaatgg cgtttcatgg
     3841 tttgtctcct tggggttggg tggaaaaagc tcggcacgga cacatcacga acagcgctcg
     3901 cgaatgaaat cgggcacagc tatcgcctgc agccgtccgc cctcggggtg gacgcaaaag
     3961 gcccgcgtct cgcggccttc gcacagcagc tcctcgccct tgaacacctg gtgcttttgc
     4021 acaaaggtct tggcagccca ggactcgacg caggtgcgca cctgcagcgt gtcgccgtag
     4081 gtggcggggc gcatgaattt ggtgtggatt tccagcaggg gcgtgccgat gatgccgtgg
     4141 ctttccttca gttcgcgcca gggcggcacg ccgcactgca tgaagaagtc gagcgacgaa
     4201 gcgtccatcc acttgctgaa gttgggaaag aaaacgatgc cggccggatc gcagtcgccg
     4261 aacatcaccc tgagctcgta ggtggtggtc ttgctcatcg tgcggccatc cggagcgcgc
     4321 cgtccaggcg gatcacctcg ccgttgaggt gcccgttggt gacgatgtgg caggccagct
     4381 cggcaaattc gctgggcttg cccaggcgcg gcgggaaggg gatggacgcg gccagcgagg
     4441 cctgcaccgg ctcgggcagg gtgcgcatca gcggcgtggc aaacaggccc ggggcgatgg
     4501 tgcacacccg gatgccgtgc tgcgccagat cgcgcgccat cggcagcgtc atgccgacca
     4561 cgccgccctt gctggcgctg taggcctgct ggcccacctg gccgtcgaag gccgccacgc
     4621 tggcggtgaa caccatggcg ccgcgctcgc cgtcttccag cggctccagc ttggcacagg
     4681 ccgcggcaaa caggcgcgca gcgttgtagg tgccgatcag gttgacgttg acgacgcgga
     4741 tgaaatcttc cagcggcgcg gcgctgccgt ccttgccgat cacccgcttg gcggtgccga
     4801 tgccggcgat ctgcatcagg atgcgcgccg gcccgtgcgc cgcggcggcc tgctcgatgg
     4861 cggcctgcat ggattcgccg ctgctcacgt cggtctggat cgccaggccg ccgatgtcgg
     4921 acgccacgcg ccgggcgttg tcgaggttga gatcgagcac cgccacccgg gcgcccaggc
     4981 gcgccagctc gcgcgccgtg gcctcgccca ggccnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnggcgcc gggccgatgc ccatgatttc gggcggcacg cccaccgccg
     5161 ccgcgccgac gatgcgcgcc agcggctgct tgccgtgggc cttggcgtag gcgccggagg
     5221 ccaccacggc cgccgccgcg gcatcgacca gggccgagga gttgcctccc gtctgcacgc
     5281 cgccttcgaa caccgggcgc agcttggcca gcacctcggg cggggaaggg cgcgggtgcg
     5341 tgtcctgcgt cagctcggtg gtcttgccct gcagcttgat gccgcgtggt ttgtagccat
     5401 ccagcgcgaa tttttcgctg accacgggca cgatctcgcc ggccagaaat ccgctggcct
     5461 gcgccgcgac ggcgcgggcg aacgaggccg aggcaaaggc gtcgacttcc tcgcgggtga
     5521 tgccgtattt cttggccagg ttctcggcgg tctggatcat gttgatgcca gcggccgggt
     5581 ctttcaaagc ctcccacatg tagtccttga agcccaccgg cgcgcccagc ttgaagccgg
     5641 tgcggtgctc gaacgcggca atcgggttgc gcgtcatgtt ctcggtgccc accaccagcg
     5701 ccgcctcgca ggcgccgccc tgcacgtgct cgccggcctg gcgcagcagc tcgaagccgg
     5761 tgccgcagat gcgctgcgcc atgatggccg gcacctccac cggcacgccg gcgtacaggc
     5821 cgatgtggcg cggcaggaag aactggtcga agtcgcccgg cgccatgttg ccgcacacca
     5881 ccgagccgat gtcgccgccc ggcacccccg cgcgctgcag ggccgcgcgc gccgccttga
     5941 tgcccagatc ggtgggcgag acgtgaccca gcgcgccgca gtagtcgacc atcggcgtgc
     6001 gcacgccgtc cagcagccag acgtcgttga aggcgttgaa aaatcccttg tgggccatgt
     6061 gggtctcctg gttcagaagc ggctgtcggg cttgatcacg atgtgccccg gcgcctcgtc
     6121 gtacagcgcc tgcaccagcg cgtcgcgctc tttcagcacg gcgcgctggt tgatgctgcc
     6181 cttgtcggtg acttcgccct tgtcgatcga agggggttcg gccagcagca tcagccgtgc
     6241 gacgcggttg gcgctgccgg tggccgtcag cgacagatcg accaccaggc tgtggaactt
     6301 ctgcagcacc gccgggtggc gcagcacatc ggtcatcggt gcgtcggcgg gcagcggcgt
     6361 cagcgcgcgc acggctgggg ttggaaagat cagcgcaccg acttcggccc ggttcaaccc
     6421 ggtgatgacc acgtcctgga tgtagggcgc accgccggtg atgacgcgtc cgcgcagcgg
     6481 cccgacgctg acgaaggtgc cggtggcgag cttgaagtcc tcggcgatgc ggccgtcgaa
     6541 gcgcaggccc aggtcgggct ggcgcgggtc gatccaggcg accgcgtcgc cggtgcagaa
     6601 gaagccttcg tcgtcgaagg cttcggccgt ggtctcgggc gcgcgccagt agccgggcgt
     6661 gatgttgggg ccgcgatagc gaatttcgag cttgtcgcct tcgggcacca gcttgagctc
     6721 aaggccgggc gcgggcaggc ccagatcgcc ggcccgcacg tcggggctgg tgacgaacag
     6781 gccaaacggg ctggattcgg tcatgcccag gccgctggcc atgacgatgc gctcgcccac
     6841 ctcgcgctcg gcgctggcaa acaggctgtc ccacaccggc tgcgccagcg aagcgccggc
     6901 gtaaaagaac atcttcacgc gcgacagcag ggtccggcgc agctggtcgt cgtgctgcat
     6961 cgcattggca atcgcctcga agccggtggg cacgttgaag tacagcgtgg gcgcgatctc
     7021 gcgcaggttg cgcagcgtct cctggatcag cgccggtgtg ggcttgccct cgtcgatgta
     7081 gagcgcgccg ccgttgtaga gcaccatgcc gacgttgtga ttgccgccga aggtgtggtt
     7141 ccagggcagc cagtcgacca gcaccggcgg ctcctcggtc agcaccggca tcgactggcg
     7201 catctgctgc tggttggcgc accacatgcg gtgggtgttg atgacggcct tgggcagctt
     7261 ggtcgagccg ctggtgaaca ggaacttgac gatggtgtcg ggcccggtgg cggcctgcgc
     7321 ggcgtcgacg gccggcgtgg cttcggtggc cagcagggtg tcgaacgcgg tggcgctgcg
     7381 cccctgcagg ctgggcgcgt cgccgtggcg cgacagcgcc agctcgcagt ctgacggcgc
     7441 cacggctgcc atggccgggc cgtagcgcgc gccgtcttcg gcccagatca ggccgggggt
     7501 gagcgtgccg acgatgtggc gcagcttgcc gtagtccttg ctgatgagcg agtaggcggg
     7561 cgaggccggg caccagggca cgcccgccca catgcagccc agcgccagca gggcgtgctc
     7621 caggctgttt tcggacagga tcagcaccgg ccgctcggcc gacaggccgc gcgccagcag
     7681 cgcctggccg atgcggcgcg cgccatccag cgcctgggcg tagctgatgc ggcgccagtc
     7741 gccaagctgg ccgtcggctg cgcgcacgcg ctgggccaga aacgtggcct cgggcgtggc
     7801 ctgcgcccag tgccgcaggc ggtcggacat gcgctcggca aagggctgca gcgcctgctc
     7861 ggcattcagg tactgcacgc cgccggcgcc gccgcgcacg cgcgcctggg ttacgccaaa
     7921 gcgcagcgga cgatagcgcg gagctgccgc ggctgcggcc cgagggatgc tcatggcggt
     7981 ttccattagc ttctgttttg atagctgctg gcgctggatg ggcaagggct gcaggccgat
     8041 ttagcttgaa gtcagccctt cttgaccttg gtgcccgcct tgccggccag aaagtcgcgc
     8101 acgcgctctt tggcctcggg cgccgactgg gcgatggcgc tgatcagcgc ctcggtcagc
     8161 aggccgtgat cggccggctg ctcggcgatg cgcggcagcg cgtgcatcag cgcgtagttg
     8221 gtcagcggcg cgttggtggc gatgcgctcg gccagctcca gcgccttgtc gaaggcgttg
     8281 ccggcgggca ccaggtactg ggcgaagccc agacgctcgc cgtcggcagc gtggtagacg
     8341 cggccggtga gcatcatgtc ggtcatgcgc gccacgccga tcagcttggg gatgcgcacc
     8401 gagccgccac cgccaacgaa gatgccgcgc gagccttcgg gcagggcgta gaaggtggtg
     8461 tcgtcggcca cgcggatgtg cgccgcgcag gccagctcca gcccgccgcc caccaccgcg
     8521 ccgtgcagcg cggccaccac cggcaccgcg ccgtactgga tggcgtccag cgccacgtgc
     8581 cagctgcgcg aatggtgcag gccggcaccc gcgtcacgtt ccttgagttc ggacagatcc
     8641 agcccggcgc agaaatgttc gccctcgcca gtgagcacgg cggcgcgcac gctggccggc
     8701 aggttctgaa aggcgtcgcg cagggccgcg accaggccgt ccgacagcgc gttgcgcttg
     8761 gccgcgcgcg tcaggcgcac cacggccagg tgttgcgcgg ggcccagcag ttcgaccgag
     8821 agatcgtggg tgacgtgcat cgtgaggtat ctccaacggc cgttattgtt tatgatgaat
     8881 aattaaatgc aaggctcgcg ggcacatctt tgctaggggt aaacacgggt gccgggcaga
     8941 cgctggcggg tgcgcgggcg tagactccag acccgcccgc cgccttgctg cgtccaccct
     9001 ctgtccggag atccatctca tgcaacgtcg acacctgctg gccggtctgg ccgccgtcac
     9061 cctgaccttt ggcccggcgt gggccaacgg ctttccctcc aggccgatcc gcatcgtggt
     9121 gcccaacgcg gccggcggcg cggccgacat caccgcgcgc acggtgggcg agcacatggc
     9181 gcgcaccctg ggccagccgg tggtggtcga gaacaagccc ggcgccggcg gcgtggtggc
     9241 cgggcaggcg gtggcgggcg cggcgcccga tggccacacg ctgctgctga tctccagcgg
     9301 cacggcggtg agccaggcgc tgttcaagtc attgccgtac gacacgctca aggccttcac
     9361 gccggtggcg ccgctggcca gcttcgatct ggtgatcgtg agcaaggagg gcggcgcctt
     9421 caagtcgctg ggcgatctga tcgcctatgc gcgccagaac cccggcaagc tcaacatcgg
     9481 cacgcccaac atcggcacca cgcagaatct ggcggccgag ctgttcaaga gcgcggccgg
     9541 gctcgatgtg caggtggtgc cgttcaaggc cacgccggac gtgatcggcg cgattcgcgg
     9601 cgaccagatc caggccgggc tggacatcgt gccgccgctt ttgacgcaga ttcaagcgaa
     9661 ggtgctgcgc ccgctggcgg tcaccggcgc caagccctcg cgcgtgctgc cgggcgtgcc
     9721 gacggcgcag gaggcgggcg tcaaaggcct gaacgccgcg tcctggaacg gcctggccgt
     9781 gccggcgggc acgcctgcgg cggtgatcga caaactcaat caggccgtca acgccgccct
     9841 gcgcgacccc gaagtggtca agaagctgca gacgctcaac ctcgatccgc accccggcag
     9901 cgcgcaggat gcgctggcca tgctgtcggg cgacattcag cgctggaacc aggtgatcga
     9961 caaggcgcgc atcgagcgca agtgagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnncggg ggtgatgacc tcggtgccgc cctatggcat tcgcgtgccc atgctgctgg
    10141 gcggcgcgca cgccgccgag ctggccgcgc ggctcggcgg ctggggcatg gacgccaggg
    10201 cggtgagcgc cgagctgggc gtggccagcg ccatcaagat gtgccgcagt gtgatgatca
    10261 agggcctgga ggcgctggtg atcgaaagct acacggcggc gcgcgcctat ggggtggaag
    10321 agcacgtgct gcccacattg caggaaacct ttcccggcat cgactggagc gcgcagggcg
    10381 cttacttctt cagccgcgtg gcgcagcacg gccagcgccg cgccgaggaa atgcgcgagt
    10441 cggcgcgcac ggtgcaggag gcgggcttcg agcctttgat gaccgcggcg attgccgcca
    10501 agcagcagtg ggtggccgac caggcgcgcg ccggggcgtt caagccgatc gtcaagggcg
    10561 cgcgctggca ggactttgcc gacgcgctgc tgcggcagcg tcaggcctga gggagccttc
    10621 gcttgctcga cattctggcc atcaccggcc ccatctacct gtgcgtcgcg ctgggctggc
    10681 tgaccacccg cgccggcctg ttcgcgcgcg ccgacatgcg ggtgctgggc cagttcgtgc
    10741 tctacatcgc gctgccggcg ctgctgttcg gcgccgtcgg tcaacggccg atcgccgaca
    10801 tcctggactg gcactacatg gcggtgttcg ccctgggcgg tctggcgacc atcggcctcg
    10861 ggctgttctg ggcgcgccgc ctggccgggc aggcgccgct ggcaggcgtg gtgcaggcca
    10921 tgggcatgag ttgccccaac tcgggcttcg tcggctaccc gatcaacctg ttggtgttgg
    10981 gcgggccgac cgccggcgtg atcctgggca tgaacatgct ggtggaaaac ctgctgctga
    11041 tcccgctgct gctggcgttg gccgacagcg ccagcggcgg cggccactgg cgcgcggtgc
    11101 tgcgtggtgt ggcggtgggc ttgtggcgca acccgctggt gctgggtctg gcggccgggc
    11161 tggcggcggc gctgctgggt tggcggctgc cgggcccgct ggagcgcacc gtgggtctgc
    11221 tggcgcagtc gagcgcggcg ctgtcgctgt tcgtcatcgg cggctcactg gccgggctgc
    11281 ggctggccgg catggggcgc acggtggcgc gcatcgccgc cggcaagctg atcgcgcacc
    11341 cgctgctgac cctgggggcg ctgtggctgc tggcagcgct gggtgcgccg ctgagcgacc
    11401 cggcattgaa gaagggcgtc gtgctggcca ccgcctgccc gatgttcggc atctacccga
    11461 ttctggccgg ccgctttggc gccgaaggcc tggccgccgc cgcactgctg ggcaccacgg
    11521 tggcgtcgtt cttcagcatc agtgcgctgc tgtggtggct gggatgaggc gccgcaccgg
    11581 gttcagatgc cggtctggcg ccgcccatgc gtggccatgc cccgtgtcgg gaatctggag
    11641 tcgggcccgt ccagatggcc ttcagaacca gcagagcgac gaggggtccg ccggccgcga
    11701 atggttcatg cgtcgtgcgg gacgcagccg ctgtcatggg ccattcgcca ttcttcggaa
    11761 cagcggcatc agggttcgag ccgctccggg tgcagcagga attcacgcag cgccacggcg
    11821 ttgttcagcg tgtcggtgcg tgcggcatag atcagcgtga cgacgccggc gcgagcgcgc
    11881 tggcgcagcg cttcgagggc cgcgggttgc tggcgcagct cggccgcgta gcgctggcga
    11941 aagccgtccc agcgcgtcgg ctcgtggccg taccactgac gcaaagcggt gctgggcgcc
    12001 acatcgcgcg cccattcggt cagcgccagc gtctgcttgc tcaggccgcg cggccacagg
    12061 cgctcgacca ggatacgcgc gccgtcgtcg gctgacgctg gttcgtaggc gcgcttgagg
    12121 cggatgcggt cggcgaggat cctgtgcgtc attgccgggc ggggccgagc tcggatgtcg
    12181 agtcaggata cgcgcacgcc agtgaggacg gtgacggcag atgtattgcc cctttccaca
    12241 gaggggcgag gggccgggtg ctactcaggg ttcatcggtc acccattgac cagcaccagc
    12301 ttgccctgca cctggcgcga gcccatgcgg gcataggcgg ccttgagctc gctcatcggc
    12361 atctgccgat cgatcagcgg cttgatcttg ccctgcgcgt accaggaggc cagctcctgc
    12421 atcatggcgg cgttggcctg cggttcgcgg cgcgcgaagt cgccccagaa cacgccgacg
    12481 atgctggcgc ccttgaccag tgccaggttg accttgagtg ccggaatctc gccggcggca
    12541 aagccgatca ccagatggcg gccgcgccag gcgatggagc ggaacgccgg ctcggtgaag
    12601 tcgccgccca ccgggtcgta gatcacgtcc gggcccttgc cgccggtcag ctccttcagg
    12661 cgttcgcgca ggtcctgggt gctgtagttg atggtgtcgt cggcgccgag ctggcggcag
    12721 aacgccagct tctcttcggt ggaggcggcg gcgatcacgc gcgcgccggc gatcttggca
    12781 atctggatgg ccgacatgcc tacgccgccg gcggcgccca gcaccagcac ggtttcgccc
    12841 gccttgagct ggccgcgatc catcagtgcg tggtggctgg tggcgtagat catgatgaag
    12901 gcggccgcgt cggccggcgg aaagcccggc ggcagcggca tgcacagctt ggccggcgcc
    12961 agcgtgtggg tggcaaagcc gccggtgccc gacaggcagg ccaccgcctg gccgaccttc
    13021 agctcggtca cgccttcgcc caccgcctcg acgacgccgg cgtactcgga gccgggcacg
    13081 aacggcagtt cgggcttgaa ctggtacttg ttctgcacga tcagcagatc gggaaagttc
    13141 aggctggcgg cctggatgcg gatcagcacc tgccctttgc caggctgggg cgtgggcagc
    13201 tcctgccagg tgagggcgtc gacgccgacg gggttttcgc aaagccatgc gtgcattgaa
    13261 tagcgctcct tgaggttcgc ggcaagggtg atggggcgcc gcggtctgca ctcgatcata
    13321 ggagcatggg ccgggagcgc catgtcccaa ccgtgacggt gaaaatccgg cggcggggcg
    13381 ggaagggcag gcctacaatc ggttgcattc gcgcattcgc gcctgacccg cccatgagaa
    13441 tcctgctctg caacgacgat ggctatcagg ccgccggcat cgtagccttt caccgggccc
    13501 tggccgcgtt gcccggcgtg caggtcgagg tggtggcgcc ggagcacaac aacagcgccc
    13561 agtccaacgc gctgaccctg cgcgcgccgc tgtatgtgaa ccaggcggcc aacggttttc
    13621 gctacgtcaa tggcacgccg gccgactgcg tgcacatcgc cctcaccggg ctgctggact
    13681 accggccgga tctggtggtg tcgggcatca acaatggcgc caacatgggc gacgacacca
    13741 tttattcggg caccgtcggc gcggccatgg aggggtacct gttcggcatc ccggcgctgg
    13801 cgttttcgct ggtcgacaag ggctgggccc acatcgacgc cgccgccgtg cgcgccgccg
    13861 agctggtggc gcgttttggt gcccacccgc cgggtggcgg caagccgtgg ctgctgaacg
    13921 tcaacatccc gaatctgccg ctggcgcaga ttcgcccacc caaggtgtgc cggctgggcc
    13981 ggcgccatgc cgcgcagtcg gccatcacca tgaccagccc gcacggcgaa accatgtact
    14041 ggatcggcgg cgccggttcg gccgccgacg cggccgaggg caccgatttc cacgccaccg
    14101 ccgagggcca tgtcgccatc actccgctga aggtggacct gaccgaccac gatgcgctga
    14161 gctactgggc gcaggggctg gggcacctgc tggcggctgg cgcggcggga ggggcaggct
    14221 gatggccacg ccgccgcggc ccggctttcc ggcgcgcatc gacctgggca cgggcggcaa
    14281 gtcggccgcc cgcccgccgg cgcgcccggc cggcgtggca ggcgccagcg ccaacccgcc
    14341 gctgaccagc gccaccgcac cgcaggcggc cagcatggca cgcgacccga tcccggcgcg
    14401 cgcccgcatg gtgcaactgc tggccgccga aggcatccgc gacccccagg tgctgcgcgc
    14461 catgggtgcg gtggaccgcc accatttcgt cgacggcgcg ctggtgccgc aggcctacga
    14521 ggacaccgcg ctgcccatcg gcctgggcca gaccatcagc aagcccagcg tggtggcgcg
    14581 catgctggag ctgctgatgc agggcgccgt ccgtggcccc gacggccgcc tgggccgcgt
    14641 gctggacatc ggcaccggct gcggctacca ggcggtggtg ctggcccagc tggccagcga
    14701 ggtctacagc atcgagcgcc tgcgcgacct gcacgacaag gcgcgcgcca acctgcgacc
    14761 gctgcgcctt gccaccgtgc acctgatcct gggcgacggc atggccggct ttgccagggg
    14821 cgcgccctat gccggcatcg tcgccgctgc cggcggtgag gccgtgcccg aagcctggat
    14881 cgaccagctg gcctggggtg ggcgcatcgt ggcgccggtg gccacggcgg cgggacagca
    14941 ggcgctgatc gtgatcgaca agtcgcgtca gggggtgcag cagacggtgc tggaagcggt
    15001 caacttcgtg cctctaaaat ccggtatcgc ttagcggatt tccagagcgc ttcaagaagg
    15061 gtttgtatgt atttgacggg acgcaggatt ttgctggccg ccacgctgct gggcggggtc
    15121 gtgctggcgg gctgctccgg caccccactc accccggcgc cggtggaaga ccgcgacacc
    15181 cgcggcaccc cgtcgcgggt cgatccggcc accttgcccg gcttcgaaaa cgccggcaag
    15241 ccgggctact acacggtgcg tccgggcgac accatcatgc gcatcgccac cgaggtcaac
    15301 cacccctggc gcgacatcgc acgctggaac aacctggaga acccgaacgt gatcgaggtc
    15361 ggccaggtgc tgcgcgtggt gccgccgcag ggcgcaaccg ccggcgcagc cacgccgatc
    15421 accgccgtgc cgctgcccgc gccgccggtc gccgccagcg gtgccaaacc tgcgccgcag
    15481 ccggtgccgg tggccacgca gaccacgccg ggcgtgagcg cgcccgcggc gccgacggcc
    15541 gcgcctgcgt cggcaccgac gccgcccgca cccgcgccag cggcggcgcc gccgtcggcc
    15601 ggggccgaca acgtcgactt catctggccc gccagcggtt cggtggtggc cggcttcgac
    15661 gaagccaaga acaagggcct gggcatcggc ggcaaggcgg gcgatccggt gctggcggcg
    15721 gccgacgggc gcgtggtcta tgccggcgcc ggtctgcgcg gctatggcaa cctgatcatc
    15781 ctcaagcaca acaacacctt cctgaccgcc tacgcccaca accagaagct gctggtcaag
    15841 gaagaccagg acgtcagaaa aggccagaag atcgccgaaa tgggctccag cgacaccgac
    15901 cgcgtcaagc tccacttcga gatccgccgc tccggcaagc cggtggaccc ggcgcgctat
    15961 ctgccggcgc gctgattccg ggcccgcggg ccggccaagg gcgctccggt gagcgccctt
    16021 cgtgtttcgg cggcctgtgg gttgctataa tatcaatagc tgctggcgct cgccagataa
    16081 gcgccggagc ttcttttcat catgaacctc gagcccgatc acagccgcga cgcggcactg
    16141 cccgaaggct ggctgcaggt cgattcgatc gacctcgaag cccagggcat cgcgcgccgg
    16201 cccgacggca aggtggtgtt catcgacggt gcgctggccg gcgagctggt gtcggtcagc
    16261 gtgcaccggc gcaagaacca atgggaagcc gccaccctga ccgagttgca ccgcgccagc
    16321 agcctgcgcg tgcgcccgcg ctgcccgcat ttcggcctgc accagggcgc ctgtggcggc
    16381 tgcaagatgc agcacctgga cgccagcgcc caggtggctg tcaagcaacg tgcgctggaa
    16441 gaccagttgt ggcatctggg caaggtcagg ccggcgctga tcctgcgccc catcgaaggc
    16501 ccggcctggg gctaccgctg gcgcgcgcgc ctgtcggtgc gctacgtggc caagcgcggt
    16561 gaggtgctgg tcggttttca cgaacgcaag agtcgctacg tggccgacat gcgcgagtgc
    16621 ccggtgctgc ccgcccacgt cagcgcgctg ctgctgccgc tgcgcgcgct gatccatggc
    16681 atgcaggcgc gcgacagctg cccccagatc gagctggcct gcggcgacga ggtcacggcg
    16741 ctggtgctgc gccacctgga gccgctcagc gccgccgacc gccagcgcct gagcgagttc
    16801 ggccgccagc acggcgtgca gtggtggctg caacccaagg gcccggacag cgtgcagcgg
    16861 ctggagccgg gcggccccga gctggcctac gcgctggcgg aattcggtct gcgcatgccg
    16921 tttcgaccga ccgacttcac ccaggtcaac ccgcacatca accgcgtgct ggtggggcgg
    16981 gcgctgcgcc tgctgcaggc ccagcggcac gagcgcgtga tcgactggtt ctgcggcctg
    17041 ggcaacttca cactgccgat cgccaccacc gctggccagg tgctgggggt ggaaggcagc
    17101 gccgccctgg tcgaccgcgc ccgccagaat catgaacaaa atcgtgctgc agcgcgcact
    17161 ggatctgcgc tggcagctac cgaatttgta gcgcgcaacc tgttcgacat gagtgccgcg
    17221 caactggtgc aggacggcag cgccgaccgc tggctggtgg acccgccgcg cgaaggtgcg
    17281 tttgcgctgg tcaaggcgct ggcggccatc caccaggggc gcctcggtgc gccggacgcc
    17341 gagccgctgc ccgccggtgc cgacggctgg cgtccgccgc gccgcatcgt ctacgtcagc
    17401 tgcaaccccg ccacgctggc gcgcgacgcc ggcctgctgg tgcatcaggc cggctaccgc
    17461 tgcagcgccg ccggggtggt caacatgttt ccgcacaccg cgcacgtcga aagcatggcg
    17521 gtgttcgacc tgcaggactg aggcggtacg ccgacaagca aaagccccgc acaacttgtg
    17581 ctgtgcgggg cttgctgttc tttggctccc cgacctgggc tcgaaccagg gacctacgga
    17641 ttaacagtcc g
//
LOCUS       DAOQTZ010000057        17443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3717_length_17443_cov_12.296066, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000057 DAOQTZ010000000
VERSION     DAOQTZ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17443
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3717_length_17443_cov_12.296066"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /locus_tag="PKN51_08195"
     CDS             <1..1841
                     /locus_tag="PKN51_08195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNT85118.1"
                     /translation="AVIADNVGDNVGDCAGMAADLFETYAVTLIATMVLGALMVTAAP
                     TQAVLYPLALGGVSIIASIIGTFFVKASPGMKNVMPALYKGLAIAGVLSLIAFYFVTS
                     WIIPDNALGGSGAVMKLFGACVVGLVLTAALVWITEYYTGTQYGPVKHVAQASTTGHA
                     TNIIAGIGVSMKSTAWPVIFVCIAIVAAYALAGLYGVAVAAMSMLSMAGIIVALDAYG
                     PITDNAGGIAEMSEMPSAVRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTH
                     KLESFGQAISFDLSDPMVIVGLFIGGLIPYLFGAMAMEAVGRAAGSVVEEVRRQFREI
                     KGIMEGTGKPEYGKAVDMLTGAAIKEMMIPSLLPVAVPILVGLVLGPKALGGLLMGTI
                     VTGLFVAISMCTGGGAWDNAKKYIEDGHHGGKGSEAHKAAVTGDTVGDPYKDTAGPAV
                     NPLIKIINIVALLIVPLVVGMHGGKADGAAPTAPAAQVSAAAPAPAVAPASASDDAAS
                     VVVEGEKVLFYFATGKADPHPDGSQALATIVNGVKAGKKAAISGYVDSTGSAAANAEV
                     AKQRALAVAALLKSLGVTDEQMQLQKPEDIQAGSGPQARRVEVTLQ"
     gene            complement(1860..3077)
                     /locus_tag="PKN51_08200"
     CDS             complement(1860..3077)
                     /locus_tag="PKN51_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT85119.1"
                     /translation="MTIDPTSRRLPLGIWVLGFVSLLMDVSSEMIHSLLPMFMVGSLG
                     MSVALVGLLEGLAEATALIAKVFSGVLSDWLGRRKRLALLGYGLSAVTKPLFALAAGP
                     AGPALVITARLMDRVGKGIRGAPRDALVADLAPPELRGAAFGLRQALDTVGAFVGPLL
                     ATVLMLLWSDHFRAVFALALVPAALCVLLLALGVREPERPAGRPRINPLSRANLVRLP
                     RAYWSVVGVGAVFTLARFSEAFLVLRAEQTGVAIALVPLVMVAMNAVYALAAYPFGKL
                     ADRLPHARLLFAGLAVLWLADGVLAVATHWSALLLGILLWGLHLAMTQGLLAAMVAAT
                     VPDDLRGTGFGLFYLVSGVAMLAASVVAGLLWQVLGPAWTFGAGAGFCALTMLVLARP
                     AARHKKSPPGGGL"
     gene            3148..4581
                     /locus_tag="PKN51_08205"
     CDS             3148..4581
                     /locus_tag="PKN51_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT85120.1"
                     /translation="MQYNFIDNASVPSLSGHTLPVLDPSDGQPFDELQRSDARDIDRA
                     VRSARACYDERWSRLSAAERGRLLMALSRKVADHADELALLEQRDCGKPTRQARADAA
                     ALARYFEFYAGACDKLHGETLPYQNGYSVFTWREPHGVTGHVIPWNYPMQIFGRSVGG
                     ALAAGNCCVVKPAEDACLSLLRVAQLAAEVGFPPGTINLVTGYGHEVGDALARHPGID
                     HISFTGSPRVGTLIQQVAAERHCPVTLELGGKSPQIIFADADLDAALPVVINAIVQNA
                     GQTCSAGSRVLIQQSIYDQMLDRLGAAFEALRVGPASMDLDVGPLIRQSQQQRVWDFL
                     SDAQNDGIATVAQGQIVDEAPEAGFYQAPVLLADVPVAHRVAQEEVFGPVLSAMPFRD
                     EDHAVELANATRFGLVAGVWTRDGARQFRMARRVRSGQVFVNNYGAGGGVELPFGGYK
                     SSGYGREKGFEALYGFTVLKTVAVQHG"
     gene            complement(4744..5442)
                     /locus_tag="PKN51_08210"
     CDS             complement(4744..5442)
                     /locus_tag="PKN51_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNT85121.1"
                     /translation="MNGSLIDDLMGQLQGPSLGPIAQQLGTDADTAGQAIAAALPMLV
                     GALGRNAQQPGGAGALFEALQRDHAGSGAMDLGGLLGGLLGGAGAGAGSAGMGGMGGM
                     GDLLGSVLGGGAPASRQLDAGGILGHILGGAQPRAEAGLSQATGLDMGQAGKLLMMLA
                     PIVMAALARRVNAGQLDAGGLGDVLGQERAQLQQQGGLGGGLMNAVLDQNGDGQLDLG
                     DLLKLGGSLLGGRR"
     gene            5553..6323
                     /locus_tag="PKN51_08215"
     CDS             5553..6323
                     /locus_tag="PKN51_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT85122.1"
                     /translation="MRAKNKSIIVTGAGGGIGEGIARRLAVEGAVVIVNDIQPTLGEK
                     VTADIVAAGGQARYVAADVTRGDDWARLVQAAQDFGGRLDVVVNNAGWTHRNRPMLEV
                     SEAEFDKVYAVNMKSIYWSAIHAVPAMRANPGGSGGSFINIASTAGLRPRPGLTWYNG
                     SKGAVIITSKSMAAELGPDNIRVNCINPVFNPDTGLSAEFAGGPVDEARRAKFLATIP
                     LGRFSTALDVANAALYLASDEAAFISGVCIEVDGARCV"
     gene            complement(6445..6993)
                     /locus_tag="PKN51_08220"
     CDS             complement(6445..6993)
                     /locus_tag="PKN51_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT85123.1"
                     /translation="MKPPIAMPALEHHTRRRWLGRLTAATAASLAAPWLCAKDAAPLP
                     LPALGSALPLVAAPLLGGGRFEPAQAEGRVLVLYWWASWCPFCAQQSPEMQKLWDAQR
                     GRGLQMLALSIDKKQGDASAYLQKKGYTFPAAWLSPDLARAYPKPDGLPVTVVRGKDG
                     RVLQAEKGQLFPEDVAGLARWL"
     gene            7069..8199
                     /gene="moaA"
                     /locus_tag="PKN51_08225"
     CDS             7069..8199
                     /gene="moaA"
                     /locus_tag="PKN51_08225"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNT85124.1"
                     /translation="MGERNIPLIDQRTAGLPIPAAAPAPGQELTDTRGRPLHDLRISV
                     TDRCNFRCTYCMPKEVFGADYPYLPHASLLTFEEITRLARLFIAHGVRKIRLTGGEPL
                     LRKNVEGLIEQLAALRTPDGEPLDITLTTNGSLLARKARALAAAGLRRVTVSLDSLDD
                     AVFQRMNDVGFPVREVLAGIEAAQAAGLAPIKVNMVVKRGTNAHEIVPMARHFRGSGM
                     ALRFIEYMDVGATNGWRMDEVLPSAEVLRRLQAEFALVPLAHSAPGETAERWGHAGAD
                     GRHDPALGEIGLISSVTRPFCGDCNRARLSTEGRLYLCLFASQGYDLRALLRGGASDE
                     AIGHAIGAIWGHRADRYSELRASGRLDPHSGERRIEMSYIGG"
     gene            8233..8919
                     /gene="mobA"
                     /locus_tag="PKN51_08230"
     CDS             8233..8919
                     /gene="mobA"
                     /locus_tag="PKN51_08230"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HNT85125.1"
                     /translation="MTAIPGAPADPDRIPITALLLAGGRGSRMGGLDKGLQSFGGLPL
                     AQHALERLRRQTLPPAAMLINANRHLPDYQAFGVPVWPDVLPDYAGPLAGFLTGLAHA
                     QTPWMLTLPCDVPRFPLSLCERLAQALLAQKADIAMVAAPEEDGRLRPQPVFCLLRRE
                     LHDSLARFMAAGGRKIDAWTAQHRQVLVPFDHPGDDPRAFANANTLAELRQLEREPRQ
                     DACQPPMPSF"
     gene            8936..9457
                     /locus_tag="PKN51_08235"
     CDS             8936..9457
                     /locus_tag="PKN51_08235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNT85126.1"
                     /translation="MPLPPLTIRLLGPADLPAYKALRDIGLRHDPEAFTTDFNAGSAL
                     PAAAYATRLGQPPEDHFILGAFDPAGAMLGAVVCERESRLKKRHEAALVGMIIAPEVR
                     SHGIGKALLAAFDQLVRQLPGLEQVVLSVTASNARAVALYEHAGFVRYGLLPRAIKID
                     GRYHDKALMVKVL"
     gene            9454..10824
                     /locus_tag="PKN51_08240"
     CDS             9454..10824
                     /locus_tag="PKN51_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNT85127.1"
                     /translation="MTPTTAATTPRTADIAAALQGYDPQALHADAVTAFLQRLVEPVT
                     EVQRVPLPEALGRVLAEDVISPISVPPHDNSAMDGFAFDGAQLQPGQPLRLRCVGTAL
                     AGTAWRAAVAAGECVKIMTGAVMPAGLDTVVPQEFTRVEGAQIELPAGVVQRGDNRRQ
                     AGEDLMAGRPALRQGERLTPAALGLLASLGLGQVTLRRRLRVAYFSTGDEILSLGQPP
                     REGAVYDSNRYTVGGLLARLGVEVIDLGVVPDQPAALRAAFGQAAAQADAVITSGGVS
                     VGEADHTKAMMRELGDVVFWRIAMRPGRPMAVGRIVTPAPELQVKSASSANTANANSY
                     QNNSNSAILFGLPGNPVAVMVTFLAFVRPALLRMMGARPEPPVWLRARSSQAIAKKPG
                     RTEYQRGVVSTQPDGQLTVRTTGNQGSGVLSSMVQANGLIVLGHAQGDVAVGDEVRVM
                     MFEGAL"
     gene            complement(10843..11655)
                     /locus_tag="PKN51_08245"
     CDS             complement(10843..11655)
                     /locus_tag="PKN51_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT85128.1"
                     /translation="MFERILVPLDGSVFSEEMLAHATGLAAVRGTPLTLMRVVDKGAD
                     EAEARAYVEALAARHGARGICVVAHGDVADTVLDEAAGVPHTLVAMSSRGRSGLMEAV
                     LGSVAQRVVRGADAPVLVYRPTGRSAAGGGAVAVRRIVLPLDGSPLSEVMGLQAAEFA
                     RWAGAELEVVRVVDATGAAGLPAGGELAALESGYVRSKATELGRSHGVPVNWDVLHGD
                     AVQAIASHVAVRSDTVLAMVTRRQGAVETALLGSVTAGCLRQAGVPVLMRAP"
     gene            complement(11738..14071)
                     /locus_tag="PKN51_08250"
     CDS             complement(11738..14071)
                     /locus_tag="PKN51_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450/oxidoreductase"
                     /protein_id="HNT85129.1"
                     /translation="MSETHPAAGACPVDHAALRDPNGRPVSAGAAAFDPFGDGYQQNP
                     PEYLRWARAQEPVFFAPQIGYWVVTRYDDIKAVFRDHETFDASVALERMTPVTPQVLD
                     ILAGYDYGMQRTMVNEVEPAHMARRRALIEPFTPAALKALEPMVRRVARQYVDRFIDD
                     GRADLVEKMFWEAPLTVGLHFLGVEEDDMALLRQYSVAHTVNIWGRPTPQEQVEVAHA
                     VGNFWQLSGRIVEKMRATPDGPGWMRYTIRKQKDHPDVVTDSYIHSMMMAIIVAAHET
                     TALGITNAVRLLLENRPIWDELCADPSLIAGAVEECLRHNGSVAAWRRYATREAEIGG
                     VRIPQGARVLVVLASGNHDERHFSDPDAIDVRRDNASEHLTFGYGSHQCMGKNLARLE
                     LQIYLEELTRRLPHLRLLPQRFDYVPNTSFRGPKQLWVEWDPALNPERAQPALLAQAQ
                     PVRLGESSRQTVTRPVVVERISRATDDVLLLRLVAANGQPLPGWSPGAHVDLECGSAD
                     RSRQYSLCSDPADSRAYEIAVLRDPDSRGGSAWIHQHLQVGDRLRLRGPRNHFRFDET
                     GGRAIFVAGGIGITPIAAMARRARQRGIDYALHYSGGSRRRMALLAELQAEHGERLIV
                     HASDEGQRLDLPALLATPRADTRIYACGPQRMLQALQDATAHWPEGALCVEHFQTAPP
                     TLDPAREHAFEVELRDSGITLQVRADQTILSALQGANIDVQCDCTEGLCGSCETRVLG
                     GDIDHRDLVLTRTERERQDRMMVCCSRARGERLVLEL"
     gene            14201..14530
                     /locus_tag="PKN51_08255"
     CDS             14201..14530
                     /locus_tag="PKN51_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNT85130.1"
                     /translation="MPYTARFTPETLSRAQVDAQTGPLVLEFGTDWCGHCQGAQPLIE
                     AAFARAGQDTPHLKVEDGRGRPLGRSFGVKLWPTLVFLKDGQEQARLVRPHDADAIAQ
                     ALGQLSA"
     gene            14868..16097
                     /locus_tag="PKN51_08260"
     CDS             14868..16097
                     /locus_tag="PKN51_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT85131.1"
                     /translation="MLKPLRHPGALAVTLAAAGVLMVTMGTRQSLGLFLSPINSSTGL
                     GMASISLAMAVAQFTWGAIQPLAGAAADRWGPRPVLIAGIVLLALGNALTPLMGSSWG
                     LVFSLGLLAAIGSGAGSFSVLIGAAAQRLPMAARGTASGVINAGGSLGQFVFAPLAQL
                     LIQVQGWMGAMYSLAVITLLALPLVGQLTRGQPARHDAVEGADAGLRATLRGALGDRS
                     YLLLHAGFFTCGFHIAFLVTHLPTEVDLCGLPASVASWSLALIGLSNIVGSLWVGHLV
                     GHHRSKMILALMYGSRALLVLVYLLLPREPWVFYLFAIGLGLTWLATVPPTAALVGKL
                     FGVRYLGTLFGMTLLSHQIGGFLGAWLGGLAITQLGDYTWMWWADIALAALAALLNLP
                     IREARVVPPAASAAEGR"
     gene            complement(16152..>17443)
                     /locus_tag="PKN51_08265"
     CDS             complement(16152..>17443)
                     /locus_tag="PKN51_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT85132.1"
                     /translation="ASPLYRERLGTERVRGSDLSRLPPVTRHELMARFDDWVTDPALR
                     LDELRDFVSDPSHVGEPWLGRYVVWESSGTSGKPGIFVQDAQAMAVYDALEAVRHRPP
                     CGRGRGMFGAFAAFDVLGVSDRHAVVTATGGHFASIVSFERLRRINPWLTSSGRSFSL
                     LQPVDELVRALNEFQPTVLGTYPTAAAMLAGEAEAGRLRIAPRCIMTGGETLGKAVRD
                     RLVQVFGAQVRNSYGASEFLPIAWECPHGQLHVNEDWVLLEPVDEQMRPMPPGQRSHS
                     VLLTNLANLVQPLIRYDMGDQVTWSGQRCSCGSALPVIEVRGRSDDVMQVPSQRRGET
                     VSLLPLALCTVIEEECGVFDFQLRQHGPSTLVLRLPLRGEAAVAAITRCADALQRFAA
                     MQGARPIRVLGEPGCDVPRGRSGKACRVAMEGSNPVQ"
BASE COUNT         2624 a         6416 c         6009 g         2394 t
ORIGIN      
        1 ccgccgtgat cgccgacaac gtgggcgaca acgtgggcga ctgcgccggc atggcggccg
       61 acctgttcga gacctacgcc gtcaccctga tcgccaccat ggtgctgggc gccctgatgg
      121 tcaccgccgc gccgacgcaa gccgtgctgt acccgctggc gctgggcggc gtgtcgatca
      181 tcgcctccat catcggcacc ttcttcgtca aggccagtcc cggcatgaag aacgtgatgc
      241 cggcgctcta caagggcctg gcgatcgccg gcgtgctgtc gctgatcgcc ttctacttcg
      301 tgaccagctg gatcatcccc gacaacgccc tgggcggcag cggcgccgtg atgaagctgt
      361 tcggcgcctg cgtggtgggc ctggtgctga cggcggcgct ggtgtggatc accgagtact
      421 acaccggcac ccagtacggc ccggtcaagc acgtggcgca ggccagcacc accggccacg
      481 ccaccaacat catcgccggc atcggcgtgt ccatgaagtc caccgcctgg ccggtgatct
      541 tcgtctgcat cgccatcgtt gcggcctatg ccctggccgg cctgtacggc gtcgcggtgg
      601 ccgccatgtc gatgctgtcg atggccggca tcatcgtcgc gctggacgcc tacggcccca
      661 tcaccgacaa cgccggcggc attgccgaaa tgagcgaaat gccgtccgcc gtgcgcgaca
      721 tcaccgaccc gctggacgcc gtcggcaaca ccaccaaggc ggtgaccaag ggctacgcca
      781 tcggctcggc gggcctcgcg gcgctggtgc tgtttgccga ctacacgcac aagctggaaa
      841 gctttgggca ggccatctcg ttcgatctgt ccgacccgat ggtgatcgtg ggcctgttca
      901 tcggcggcct gatcccctac ctgttcggcg ccatggccat ggaagccgtc ggccgcgccg
      961 ccggcagcgt ggtcgaagag gtgcgccgcc agttccgcga gatcaagggc atcatggaag
     1021 gcaccggcaa acccgaatac ggcaaggccg tcgacatgct gaccggcgcg gccatcaagg
     1081 aaatgatgat ccccagcctg ctgccggtgg cggtgccgat cctggtcggc ctggtgctgg
     1141 ggccgaaggc gctgggcggc ctgctgatgg gcaccatcgt caccggcctg ttcgtcgcca
     1201 tctccatgtg caccggcggc ggcgcctggg acaacgccaa gaagtacatc gaggacggcc
     1261 accacggcgg caagggcagc gaggcgcaca aggccgccgt caccggcgac accgtgggcg
     1321 acccctacaa ggacaccgcc ggcccggccg tcaacccgct gatcaagatc atcaacatcg
     1381 tggcgctgct gatcgtgccg ctggtggtgg gcatgcatgg cggcaaggcc gacggcgccg
     1441 cccccacggc gccggcagcc caggtcagcg ctgcggcgcc ggcccctgcc gtagccccgg
     1501 ccagcgcgag cgacgatgcc gcctcggtgg tggtcgaagg tgagaaagtg ctgttctact
     1561 tcgccaccgg caaggccgac ccgcacccgg acggcagcca ggcgctggcc accatcgtca
     1621 acggcgtcaa ggccggcaag aaggccgcca tcagcggcta cgtggacagc accggcagcg
     1681 ccgccgccaa cgccgaagtc gccaagcagc gcgccctggc cgtggccgcg ctgctcaaga
     1741 gcctgggcgt gaccgacgag cagatgcagt tgcaaaagcc cgaagacatc caggccggca
     1801 gcggcccgca ggcgcgccgc gtcgaggtga cgttgcagta agcgcgccca ccggccacat
     1861 caaagcccgc cgcccggcgg gctttttttg tgccgtgcgg ccgggcgggc gagcaccagc
     1921 atcgtcagtg cacaaaaccc ggccccggcg ccaaacgtcc atgccggccc cagaacctgc
     1981 cacagcaggc ccgccacgac gctggctgcc agcatggcca cgccactgac caggtagaac
     2041 aagccaaagc cggtgccgcg caagtcgtca ggcaccgtgg cggccaccat ggccgccagc
     2101 agcccctgcg tcatcgccag gtgcagaccc catagcagga tgcccagcag cagcgccgac
     2161 cagtgggtgg ccacggccag cacgccatcg gccagccaca gcaccgcgag tccggcgaac
     2221 agcaggcggg cgtggggcag gcggtcggcc agcttgccaa aggggtaggc cgccagcgcg
     2281 tacaccgcgt tcatggccac catcaccagc ggcaccagcg cgatcgccac gccggtctgc
     2341 tcggcgcgca gcaccagaaa ggcctccgaa aagcgcgcca gcgtgaacac cgcgcccacg
     2401 ccgaccaccg accagtaggc gcgcggcaga cgcaccaggt tggcgcggct gagagggttg
     2461 atgcggggcc tgccggcggg gcgctcgggt tcgcgcacgc ccagggccag cagcagcaca
     2521 cacaacgccg ccggcacaag ggccagcgcg aacacggcgc ggaagtggtc ggaccacagc
     2581 agcatcagca ccgtcgccag cagcgggccg acgaaggcgc ccacggtgtc cagcgcctga
     2641 cgcaggccga acgccgcgcc gcgcagttcg ggcggagcca gatcagccac cagcgcatcg
     2701 cgcggcgcgc cgcgaatgcc cttgccgacc cggtccatca ggcgcgcggt gatcaccagc
     2761 gccggccccg ccggcccggc ggccagggca aacagcggct tggtcaccgc gctgaggccg
     2821 tagccgagca acgccagccg cttgcgtcga cccagccagt cgctcagcac acccgagaac
     2881 accttggcaa tcagcgccgt ggcctcggcc aggccttcca gcaggccgac cagggccacg
     2941 ctcatgccca gcgagccgac catgaacatc ggcagcaggc tgtggatcat ctcggaggag
     3001 acatccatca gcaggctgac gaagcccagc acccagatgc ccaagggcag gcgccgggac
     3061 gttggatcga tggtcatggt gatgcaccca cgcggacggc gccgatggtg ccgcgcgatc
     3121 cgtgccgcac ggcggcacaa taccgccatg caatacaact tcatcgacaa cgcctccgtg
     3181 ccgtccctgt cgggccacac cctgccggtg ctcgacccct ccgacggtca gcccttcgac
     3241 gaactgcagc gcagcgacgc gcgcgacatc gaccgcgccg tgcgctcggc gcgcgcctgc
     3301 tacgacgagc gctggagccg cctgtcggcc gccgaacgcg ggcgcctgct gatggcgctg
     3361 tcgcgcaagg tcgccgacca tgccgacgag ctggcgctgc tggagcagcg cgactgcggc
     3421 aaacccaccc gacaggcgcg cgccgacgcc gccgcgctgg cgcgctactt cgagttctac
     3481 gccggcgcct gcgacaagct gcatggcgaa accctgccct accagaatgg ctacagcgtg
     3541 ttcacctggc gcgagccgca tggcgtgacc ggccacgtga tcccctggaa ctacccgatg
     3601 cagatcttcg gccgcagcgt gggcggtgcg ctggcggcgg gcaactgctg cgtggtcaag
     3661 ccggccgagg acgcctgcct gtcgttgctg cgggtggcgc aactggcggc ggaagtgggc
     3721 tttccgccag gcaccatcaa cctggtcacc ggctacggcc acgaggtggg cgacgcactg
     3781 gcgcgccacc ccggcatcga ccacatcagc ttcaccggca gcccgcgcgt gggcacgctg
     3841 atccagcagg tggccgccga gcgccactgc ccggtgacgc tggagctggg cggcaagagc
     3901 ccacagatca tctttgccga cgccgacctg gacgccgccc tgccggtggt catcaacgcc
     3961 atcgtgcaga acgccggtca gacttgctcg gccggttcgc gcgtgctgat ccagcaaagc
     4021 atttacgacc agatgctcga ccgcctgggt gccgccttcg aggccctgcg cgtgggcccg
     4081 gccagcatgg acctggacgt cggccccctg atccgtcaga gccagcaaca gcgcgtatgg
     4141 gactttcttt cggacgccca gaacgacggc atcgcgacgg tggcgcaggg ccagatcgtc
     4201 gacgaagcgc ccgaagccgg cttctaccag gccccggtgc tgctggccga cgtgccggtg
     4261 gcgcaccgcg tggcgcagga agaggtgttc ggcccggtgc tgtcggcgat gccgtttcgc
     4321 gacgaggacc acgcggtgga actggccaac gccacccgtt tcggcctggt ggccggtgtc
     4381 tggacgcgcg acggcgcgcg ccagttccgc atggcgcggc gcgttcgcag cggtcaggtg
     4441 ttcgtcaaca actacggcgc cggcggtggc gtggaactgc cctttggcgg ctacaagtcg
     4501 tccggctatg ggcgcgaaaa gggcttcgag gcgctgtacg gcttcacggt gctcaagacg
     4561 gtcgccgtac agcacgggtg atcaggtcgg acgcccgggc gtccccgcgc agcaggcacc
     4621 tcggtatcgc agcgcacgca aaagacaacc gcatctttca gcgtcttcgg cgccatcgct
     4681 gcgtcctctg cgtgcggggc atatcctgtc gcgccgccgc ccgaagcgca acaggccagt
     4741 ccttcagcgc cgaccgccga gcaggctgcc gccaagcttg agcaggtcgc ccagatcgag
     4801 ttggccatcg ccgttctgat ccagcacggc gttcatcagg ccgccgccca ggccgccctg
     4861 ctgctgcagc tgggcacgct cctgccccag cacatcgccc agtccgccgg catcgagctg
     4921 gccagcgttg actcgcctgg ccagcgcggc catcacgatg ggcgccagca tcatcagcag
     4981 cttgccggcc tggcccatgt ccagaccggt ggcctgactc aggcccgcct cggcgcgtgg
     5041 ctgcgcgccg cccaggatgt gacccaaaat gccaccggca tcgagctgcc ggctggcggg
     5101 cgcgccgccg cccagcacgc tgcccagcag atcacccatg ccacccatgc cacccatgcc
     5161 tgcgctgccg gcgcccgcgc cggcgccgcc cagcaggcca ccgagcagcc cgccgagatc
     5221 catcgcgccg ctgcccgcgt ggtcgcgttg cagcgcctcg aacagggcac cggcgccgcc
     5281 aggttgctgc gcgttgcgcc ccagcgcgcc caccagcatg ggcagtgcgg cggcaatggc
     5341 ctggccggcg gtgtcggcgt cggtgcccag ctgctgcgcg atcgggccca gcgaggggcc
     5401 ctgcagttgt cccatcaggt cgtcaatcag tgatccgttc atgctcgaac cctccggtgc
     5461 atcggtgaag acctgacatc gtaacgacgt cacggcagcg gccgcgtaac atccggtccg
     5521 aactgccaac tgcgtcacaa ggagcaaagc acatgcgggc aaaaaacaaa tccatcatcg
     5581 tcaccggcgc cggcggcggc atcggcgagg gcattgcccg ccggctggcg gtcgaggggg
     5641 cggtggtgat cgtcaacgac atccagccga ccctgggcga aaaagtcacc gccgacatcg
     5701 tggcggccgg tgggcaggcg cgctacgtcg ccgccgacgt cacgcgcggc gacgactggg
     5761 cgcgtctggt gcaggccgcg caggacttcg gcgggcgcct ggacgtggtg gtcaacaacg
     5821 ccggctggac gcaccgcaac cgccccatgc tggaagtgag cgaggccgag ttcgacaagg
     5881 tctacgccgt caacatgaag agcatctact ggagcgccat ccacgccgtg ccggccatgc
     5941 gtgccaaccc gggcggcagc ggcggcagct tcatcaacat cgcctccacc gccggcctgc
     6001 gcccgcgccc gggcctgacc tggtacaacg gcagcaaggg cgcggtcatc atcaccagca
     6061 agtcgatggc ggccgagctg gggcccgaca acatccgcgt caactgcatc aacccggtgt
     6121 tcaaccccga caccggcctg tcggccgaat tcgccggcgg cccggtggac gaggcgcggc
     6181 gcgcgaaatt cctggccacc atcccgctgg ggcgcttttc caccgcgctg gacgtcgcca
     6241 acgccgcgct gtatctggcc agcgacgagg cggccttcat cagcggcgtg tgcatcgagg
     6301 tggacggcgc acgctgcgtc tgaacacggc cgggcgccat gacgcatgac ctcgcggcgc
     6361 ctccacgaca acgccggaga tcagaaagaa atcaggcgcc agcccaatgg agaagaccgc
     6421 cagatgctac attttatgta gcactcagag ccagcgcgcc agaccggcca catcctcggg
     6481 aaacaactgc cccttctcgg cctgcagcac acgcccgtcc ttgccgcgca ccacggtgac
     6541 cggcaggcca tcgggtttgg ggtaggcgcg ggccagatcg gggctcagcc aggcggccgg
     6601 aaacgtgtag ccctttttct gcaggtaggc gctggcgtcg ccctgcttct tgtcgatcga
     6661 cagcgccagc atctgcaggc cgcgcccgcg ctgggcgtcc cacagtttct gcatttcagg
     6721 gctttgctgg gcgcaaaacg ggcaccagct ggcccaccag tacagcacca gcacgcggcc
     6781 ttcggcctgc gccggctcga agcgcccgcc gcccagcagc ggcgccgcca ccagcggcag
     6841 cgcactgccc agcgccggca acggcagggg cgcggcgtcc ttggcgcaca gccagggcgc
     6901 ggccagactg gccgccgtgg cggcggtcag gcggcccagc cagcggcggc gggtgtggtg
     6961 ctcgagtgcg ggcatggcga tgggcggttt catgatgtcg atcaatggta gcgcggcgcc
     7021 acggcctgcg gacgtcaggg gcatcggcgc ttgcggataa tggccgccat gggagagcgc
     7081 aacattcccc tcatcgacca gcgcacggcg gggctgccga tcccggcagc ggcgccggcg
     7141 ccgggccagg aattgaccga cacccgcgga cgcccgctgc acgatctgcg catcagcgtc
     7201 accgaccgct gcaacttccg ctgcacctac tgcatgccca aggaggtgtt cggcgccgac
     7261 tacccctacc tgccgcacgc gtcgctgctg acgttcgagg aaatcacgcg cctggcgcgc
     7321 ctgttcatcg cccacggcgt gcgcaagatc cgcctgaccg gcggtgagcc gctgctgcgc
     7381 aagaacgtcg agggtctgat cgagcagctg gcggcgctgc gcacgcccga cggcgagccg
     7441 ctggacatca ccctcaccac caacggctcg ctgctggcgc gcaaggcccg cgcgctggcg
     7501 gcggcgggcc tcaggcgcgt cacggtcagc ctggacagcc tggacgacgc ggtgttccag
     7561 cgcatgaacg acgtcggctt tccggtgcgc gaggtgctgg ccggcatcga ggcggcgcag
     7621 gccgccgggc tggcgccgat caaggtcaac atggtggtca agcgcggcac caacgcgcac
     7681 gagatcgtgc ccatggcgcg gcactttcgc ggcagcggca tggcgctgcg cttcatcgaa
     7741 tacatggacg tgggcgccac caacggctgg cgcatggacg aggtgctgcc ctcggccgag
     7801 gtgctgcggc gcctgcaggc cgaattcgcc ctggtgccgc tggcgcacag cgcgccgggc
     7861 gaaaccgccg agcgctgggg ccatgccggc gccgatggcc gccacgaccc ggcgctgggc
     7921 gagatcggcc tcatcagcag cgtcacccgc ccgttctgcg gcgactgcaa ccgcgcccgc
     7981 ctgtccaccg aagggcggct gtacctgtgc ctgtttgcca gccagggcta cgacctgcgc
     8041 gccctgctgc gcggcggcgc cagcgacgag gccatcggcc acgccatcgg cgccatctgg
     8101 ggccatcgcg ccgaccgcta cagcgagctg cgcgccagcg gccggctcga cccgcacagc
     8161 ggcgagcggc gcatcgagat gagctacatc ggtggctgac cccgcgcccc gggcctggcc
     8221 gagactcgcg tcatgaccgc catccctggc gcccctgccg atccggaccg gatccccatc
     8281 accgccctgc tgctggccgg cggccgcggc agccgcatgg gtgggctgga caagggcctg
     8341 cagagcttcg gcggcctgcc gctggcccag catgcgctgg agcgtttgcg ccggcagacg
     8401 ttgccgcccg ccgccatgct gatcaacgcc aaccgccacc tgcccgacta ccaggccttc
     8461 ggcgtgccgg tgtggcccga cgtgctgccc gattacgccg gcccgctggc cgggttcctg
     8521 accgggctgg cgcatgccca gacgccctgg atgctcaccc tgccgtgcga cgtgccgcgt
     8581 tttccgctat cgctttgcga gcgcctggcg caagccctgc ttgcccaaaa ggccgatatc
     8641 gccatggtcg cggcgcccga ggaggacggc cggctgcgcc cccagccggt gttctgcctg
     8701 ctgcggcgcg agttgcacga cagcctggcg cgcttcatgg cggcgggcgg gcgcaagatc
     8761 gacgcctgga ccgcgcagca ccggcaggtg ctggtgccgt tcgaccaccc gggcgacgac
     8821 ccgcgcgcct ttgccaacgc caacacgctg gccgagctgc gccagctgga gcgcgaaccc
     8881 cgacaagacg catgccagcc ccccatgccc agcttctgac cccggagaca ccaccatgcc
     8941 gctgccgccg ctgaccatcc gcctgctcgg cccggccgac ctgcccgcct acaaggcgct
     9001 gcgcgacatc ggcctgcgcc acgacccgga ggccttcacc accgacttca acgccggctc
     9061 ggccctgccg gccgccgcct acgccacgcg cctggggcaa ccgccggaag accacttcat
     9121 cctgggcgcc ttcgaccccg ccggcgccat gctgggcgcc gtggtgtgcg agcgcgagtc
     9181 gcgcctgaaa aaacgccacg aagccgccct ggtgggcatg atcatcgccc ccgaggtgcg
     9241 cagccacggc atcggcaagg cgctgctggc cgcgttcgac cagctggtgc gccagctgcc
     9301 ggggctggag caggtggtgc tgtcggtcac cgccagcaac gcgcgcgccg tggccctgta
     9361 cgagcacgcc ggcttcgtgc gctatggcct gctgccgcgc gccatcaaga tcgatggccg
     9421 ctaccacgac aaggcgctga tggtcaaagt gctatgaccc cgaccacggc cgccaccacc
     9481 ccgcgcaccg ccgacatcgc cgccgcgctg cagggctacg acccgcaggc gctgcacgcc
     9541 gacgccgtga ccgcctttct gcagcgcctg gtcgagcccg tcaccgaagt gcagcgcgtg
     9601 ccgctgcccg aggcgctggg ccgggtgctg gccgaagacg tgatctcgcc catcagcgtg
     9661 ccgccgcacg acaactcggc catggacggc tttgccttcg acggcgcgca actgcaaccc
     9721 ggccagccgc tgcgcctgcg ctgcgtcggc acggcgctgg ccggcaccgc ctggcgcgct
     9781 gcggtggcgg ccggcgaatg cgtgaagatc atgaccggcg ccgtcatgcc ggccgggctg
     9841 gacaccgtgg tgccgcagga attcacccgc gtcgagggcg cgcagatcga gctgccggcc
     9901 ggcgtggtgc agcgcggcga caaccgccgc caggccggcg aagacctgat ggccggccgc
     9961 ccggcgctgc gccagggcga gcgcctgacc ccggccgcgc tcggcctgct ggccagcctc
    10021 gggctgggcc aggtgacgct gcggcggcgc ctgcgcgtgg cctacttctc gaccggcgac
    10081 gaaatcctgt cgctcggcca gcccccccgc gaaggcgcgg tgtacgacag caaccgctac
    10141 acggtcggcg gcctgctggc acgcctgggg gtcgaagtga tcgacctcgg cgtggtgccc
    10201 gaccagccgg ccgcgctgcg ggcggcgttc ggccaggccg ccgcacaggc cgatgccgtc
    10261 atcaccagcg gcggcgtcag cgtgggcgaa gccgaccaca ccaaggccat gatgcgcgag
    10321 ctgggcgacg tggtgttctg gcgcatcgcc atgcggccag gccggccgat ggcggtcggg
    10381 cggatcgtca ccccggcccc ggagttacaa gtcaaatcgg cctccagcgc aaacacagca
    10441 aacgccaaca gctatcaaaa caatagtaat tcagcgatcc tgttcggcct gccgggcaac
    10501 ccggtggcgg tgatggtgac ctttctggcc ttcgtgcggc cggcgctgct gcgcatgatg
    10561 ggcgcgcggc ccgagccgcc ggtgtggctg cgcgcgcgca gcagccaggc gatcgccaag
    10621 aagcccggcc gcaccgaata ccagcgcggc gtcgtcagca cccagcccga tggccagctg
    10681 acggtgcgca ccaccggcaa ccagggctcg ggcgtgctgt cgtccatggt gcaggccaac
    10741 ggactgatcg tgctcggcca cgcccagggc gacgtggcgg tgggcgacga agtgcgggtg
    10801 atgatgttcg agggcgcgct gtagccccgc ccccgcggcg actcagggcg cgcgcatcag
    10861 caccggcacg ccggcctggc gcaggcagcc ggcggtcacg ctgcccagca gggcggtttc
    10921 gaccgcgccc tggcggcgcg tgaccatcgc cagcacggtg tcgctgcgca cggccacgtg
    10981 gctggcaatg gcctgcaccg cgtcgccatg cagcacgtcc cagttgaccg gcacgccgtg
    11041 gctgcgaccc agctcggtgg ccttgctgcg cacgtagccc gattccagcg ccgccaactc
    11101 gccgcccgcc ggcagtccgg ccgcgccggt ggcgtcgacc acccgcacca cctccagctc
    11161 ggcgccggcc cagcgggcga attcggccgc ctgcaggccc atgacctcgg acagcgggct
    11221 gccgtcgagc ggcagcacga tgcgccgcac cgccaccgcg ccgccgccgg cggccgagcg
    11281 accagtcggc cggtacacca gcaccggcgc gtcggcaccg cgcaccacgc gctgcgccac
    11341 gctgcccagc accgcctcca tcaggcccga gcgcccgcgc gaggacatcg ccaccagcgt
    11401 gtgcggcaca cccgcagcct cgtccagcac cgtgtcggcc acgtcgccat gcgccaccac
    11461 gcagatgccg cgtgccccat ggcgggccgc cagagcctcg acgtaggcgc gcgcctcggc
    11521 ctcgtcggcg cccttgtcca ccacccgcat cagcgtcagc ggcgtgccgc gcaccgccgc
    11581 caggccggtg gcgtgggcca gcatctcttc ggaaaacacc gagccatcca ggggcaccag
    11641 aatccgttcg aacatgcgct ctcctgcgtc cttgacactg caccggcccc gagtgtaggc
    11701 caggccgccc ccgggctggg tcaccctaga tccagggtca cagctccagc accaggcgct
    11761 cgccgcgggc gcgcgagcag cacaccatca tgcggtcctg gcgctcgcgc tcggtgcgcg
    11821 tcagcaccag gtcgcggtgg tcgatgtcgc cacccagcac gcgcgtctcg caggagccgc
    11881 acagaccttc ggtgcaatcg cactgcacgt cgatgttggc gccctgcagc gccgacagga
    11941 tggtctggtc ggcacgcacc tgcagggtga tgccggagtc cctgagctcg acctcgaagg
    12001 cgtgctcccg ggccggatcg agggtgggcg gcgcggtctg gaaatgctcg acgcacagcg
    12061 caccttcggg ccagtgggcg gtggcgtcct gcagcgcctg cagcatgcgc tgcgggccgc
    12121 aggcatagat gcgggtgtcg gcgcgcggcg tggccagcag cgccggcagg tcgaggcgct
    12181 ggccctcgtc gctggcatgc acgatcaggc gctcgccgtg ttcggcctgc agttcggcca
    12241 gcagggccat gcggcggcgg ctgccgccgc tgtagtgcag tgcgtagtcg atgccgcgct
    12301 gccgggcgcg ccgcgccatg gcggcgatcg gcgtgatgcc gatgccgccg gccacgaaga
    12361 tggcgcggcc gcccgtttcg tcgaaacgga agtggttgcg cgggccgcgc aggcgcagcc
    12421 ggtcgccgac ctgcagatgc tggtggatcc aggccgaccc gccacggctg tcggggtcgc
    12481 gcagcacggc gatctcgtag gcgcggctgt cggccgggtc gctgcacagc gagtactggc
    12541 gcgaacggtc ggcgctgccg cattcgaggt cgacatgggc gcccggcgac cagccgggca
    12601 gcggctggcc gttggcggcc accaggcgca gcagcagcac gtcgtcggtg gcacggctga
    12661 tgcgctcgac gaccaccggg cgggtgacgg tctggcggct ggattcgccc agccgcaccg
    12721 gctgcgcctg cgccagcagc gcgggctggg cacgctcggg gttgagcgcc gggtcccact
    12781 cgacccacag ctgcttgggc ccgcgaaacg aggtgttggg cacgtagtcg aagcgctgcg
    12841 gcagcaggcg caggtgcggc aggcggcgcg tcagctcctc caggtagatc tgcaactcca
    12901 gccgcgccag gttcttgccc atgcactggt gcgagccgta gccgaaggtc aggtgctcgc
    12961 tggcgttgtc gcgccgcacg tcgatggcgt cggggtcgct gaagtggcgc tcgtcatggt
    13021 tgcccgaggc cagcaccacc agcacgcgcg cgccctgggg aatgcgcacg ccgccgatct
    13081 cggcctcgcg cgtggcgtag cgccgccagg ccgccaccga gccgttgtgg cgcaggcact
    13141 cctcgaccgc gccggcgatc aggctggggt cggcgcacag ttcgtcccag atcgggcggt
    13201 tttccagcag caggcggacc gcgttggtga tgcccagggc cgtggtttcg tgcgccgcca
    13261 cgatgatggc catcatcatc gagtggatgt aggagtcggt gacgacgtcg gggtggtcct
    13321 tctgcttgcg gatggtgtag cgcatccagc ccggcccgtc gggcgtggcg cgcatcttct
    13381 cgacgatgcg ccccgacagc tgccagaagt tgcccaccgc gtgcgccacc tcgacctgct
    13441 cctgcggcgt cggccgaccc cagatgttga cggtatgcgc caccgaatac tggcgcagca
    13501 gcgccatgtc gtcttcctcc acccccagga agtgcaggcc gacggtgagc ggcgcctccc
    13561 agaacatctt ctcgaccagg tcggcgcggc cgtcgtcgat gaagcggtcc acgtactggc
    13621 gcgccacgcg ccgcaccatg ggttcgagcg ccttcagcgc cgccggcgtg aagggctcga
    13681 tcagcgcccg ccggcgcgcc atgtgggccg gctccacttc gttgaccatg gtgcgctgca
    13741 tgccgtagtc gtagccggcc aggatgtcca gcacctgcgg cgtcaccggg gtcatgcgct
    13801 ccagcgccac cgaggcgtcg aaggtttcgt ggtcgcggaa caccgccttg atgtcgtcgt
    13861 agcgcgtgac cacccagtag ccgatctgcg gcgcaaagaa caccggctcc tgggcgcgcg
    13921 cccagcgcag gtactcgggc gggttctgct ggtagccgtc gccgaacggg tcgaaggcgg
    13981 cggcgccggc gctgaccggg cggccgttgg ggtcgcgcag ggcggcatgg tccaccgggc
    14041 aggcgccggc ggcagggtgg gtctcgctca tggtggtgct cttgctcgaa gccagggaac
    14101 gcaaggctac tacgttgcac ggccatcggc caggcgccgc tgcagcgcct cggcgcgctg
    14161 gctatgatgc ccgggcaccg atcaccgcag cgcagccgcc atgccctaca ccgcccgctt
    14221 cacccccgag accctgagcc gcgcccaggt cgacgcccag accggcccgc tggtgctgga
    14281 attcggcacc gactggtgcg gccattgcca gggcgcgcaa ccgctgatcg aggcggcctt
    14341 cgcgcgcgcc ggccaggaca cgccgcacct caaggtggag gacgggcgcg ggcggccgct
    14401 gggccgcagc ttcggcgtca agctgtggcc gaccctggtg ttcctgaagg acggccagga
    14461 acaggcgcgc ctggtgcgcc cgcacgacgc cgacgccatc gcccaggcgc tgggccagct
    14521 cagcgcctga tgcgaaccgc ccatgaggga gagcctcgct gcgcttcgat ggcccatggg
    14581 tcatgttcct gaacgccatg ccgcatgcac accgcgccac cctggccact gcccccgatc
    14641 gccccgaccg cacgcccggc gcggcctggg tgggtggacg gcacctgcgg cggggccgac
    14701 acccgccggg ccgggcccct cgagcggcac ggccgcccag cccgtcacgg cgccctggaa
    14761 catgaacaaa atcatgttcc agcgcatgac tggcaagcgc cagcagctat tgttttgata
    14821 gtatccgggc accccgcccg gccccggcca gaggccagaa ggaccccatg ctgaaaccac
    14881 tccgacatcc cggcgcactg gcggtgacgc tggccgccgc cggcgtgctg atggtgacca
    14941 tgggcacgcg ccagtcgctg ggcctgtttc tgagcccgat caacagcagc accgggctgg
    15001 gcatggccag catcagcctg gccatggcgg tggcgcagtt cacctggggc gccattcagc
    15061 cgctggccgg tgcggcggcc gaccgctggg ggccgcgacc ggtgctgatc gccggcatcg
    15121 tgctgctggc gctgggcaac gcgctgacgc cgctgatggg ttcgtcctgg ggtctggtgt
    15181 tcagcctggg cctgctggcg gccatcggtt cgggcgcggg cagcttttcg gtgctgatcg
    15241 gcgccgccgc ccagcgcctg ccgatggcgg cgcgcggcac ggcctcgggc gtcatcaacg
    15301 ccggtggttc gctggggcag ttcgtgttcg cgccgctggc gcagctgctg atccaggtgc
    15361 agggctggat gggggccatg tattcgctgg ccgtcatcac cctgctggcg ctgccgctgg
    15421 tcggtcagct cacgcgcggc cagccggcgc ggcacgacgc cgtcgaaggc gccgacgccg
    15481 gcctgcgcgc cacgctgcgc ggcgcgctgg gcgaccgcag ctacctgctg ctgcacgcgg
    15541 gctttttcac ctgcggcttt cacatcgcct tcctggtcac gcacctgccg accgaggtgg
    15601 acctgtgcgg cctgccggcc tcggtggcca gctggtcgct ggcgctgatc ggcctgtcca
    15661 acatcgtcgg cagcctgtgg gtcggccacc tggtgggcca ccaccgcagc aagatgatcc
    15721 tggccctcat gtacggcagc cgggccttgc tggtgctggt ctatctgctg ctgccgcgcg
    15781 agccctgggt gttctacctg ttcgccatcg gcctgggcct gacctggctg gccacggtgc
    15841 cacccaccgc cgccctggtg ggcaagctgt tcggcgtgcg ctacctgggc acgctgttcg
    15901 gcatgacgct gctgagccac cagatcggcg gttttctggg cgcctggctg ggcgggctgg
    15961 ccatcaccca gctgggcgac tacacatgga tgtggtgggc cgacatcgcg ctggcggcgc
    16021 tggccgcgct gctgaacctg ccgatccgcg aggcgcgcgt ggtgccgccg gcagcgtcgg
    16081 ccgccgaagg caggtgatct tgaggtgatt tcgtgcctca ggccttgcca gtcaagcgcc
    16141 ggaagctatc attattgaac cgggtttgac ccctccatcg ccacccggca ggccttgccg
    16201 ctgcgcccgc gcggcacgtc gcagccgggt tcacccagca cgcggatcgg ccgcgcgccc
    16261 tgcatggcgg caaagcgctg cagggcatcg gcacagcggg tgatggcggc gacggccgct
    16321 tcgccgcgca ggggcaggcg cagcaccagg gtgctggggc cgtgctggcg cagctgaaag
    16381 tcgaacaccc cgcactcctc ctcgatcacg gtgcacagcg ccagcggcag caggctgacg
    16441 gtctcgccac ggcgctggct gggcacctgc atgacatcgt cgctgcgccc acgcacctcg
    16501 atcaccggca gcgccgagcc gcagctgcag cgctggccgc tccaggtgac ctggtcgccc
    16561 atgtcgtagc ggatcagcgg ctgcaccagg ttggccaggt tggtcagcag cacgctgtgc
    16621 gaacgctggc ccggcggcat cgggcgcatc tgctcgtcca ccggctccag cagcacccag
    16681 tcctcgttca cgtgcagctg gccgtgcggg cattcccagg cgatcggcag gaactccgac
    16741 gcaccgtagc tgttgcgcac ctgcgcgccg aacacctgca ccagccggtc gcgcaccgcc
    16801 ttgcccaggg tttcgccgcc ggtcatgatg cagcgtggcg caatgcgcag ccggccggct
    16861 tcggcctcgc ccgccagcat ggccgccgcc gtcgggtagg tgcccagcac cgtgggctga
    16921 aactcattga gcgcccgcac caactcgtcg accggctgca gcagactgaa gctgcgcccc
    16981 gacgaggtca gccaggggtt gatgcggcgc agccgctcga agctgacgat gctggcgaaa
    17041 tgcccgccgg tggccgtcac caccgcgtgc cggtcgctca cgcccagcac gtcgaaggcg
    17101 gcgaaggcgc cgaacatccc gcgcccgcgg ccgcacggcg gccggtggcg caccgcctcc
    17161 agcgcgtcgt acaccgccat ggcctgcgcg tcctgcacga agatgcccgg cttgccgctg
    17221 gtgcccgagc tttcccacac cacgtagcgc cccagccagg gctcgccgac gtgcgagggg
    17281 tcgctgacga agtcgcgcaa ttcgtccagc cgcagtgccg ggtcggtcac ccagtcgtca
    17341 aagcgcgcca tcagctcgtg ccgcgtcacc ggcggcaggc gcgacaggtc ggagccgcgc
    17401 acccgttcgg tgcccaggcg ctcgcggtac agcggcgagg cgc
//
LOCUS       DAOQTZ010000058        17378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3737_length_17378_cov_12.889627, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000058 DAOQTZ010000000
VERSION     DAOQTZ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17378
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_17378_cov_12.889627"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="PKN51_08270"
     CDS             complement(<1..1736)
                     /locus_tag="PKN51_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT85133.1"
                     /translation="MRLFPAVLLAAFSLPVWAAHGYALWGELKYPPGFAHFDYVNPAA
                     PKGGEIRLVGNSRASTFDKYNPFTIKGSAPTYLADLLFDSLLAGSLDEAGAAYGLLAE
                     DVSVAPDRLSATFRLRAQARFHNGDPVLARDVQHSFQTLIGPHTSPAYKTLLAEVAGV
                     DVLDERTVRYRFRQPNRELPLTVGGLPVFSHKWGQGKPFDQVVMDEPIGSGPYRIGPV
                     KFGKDITYVRDASYWAQGLNVRRGTGNFDRVQVRIYRDNTARLEALKAGEFDLMAFYS
                     AGDWARRVNGPKFRSGELVKGEFRHRLPTGFQSYVLNSRRPLLHDRRVRQALGLAIDY
                     EWMNRQMFYQSYMRVRGIFGNTECAASGMPDEAQLALMTPFRADIPPAAFGPAFEPPR
                     TDGSDNGLRENLRQAQRLLAEAGWRVQGGVLTNAQGQPLVLEYLDSNEGGARVVTPWM
                     RNLEKLGIRLNFRPVDFALYQQRLQKFDFDITTIAHQGTLNPGQEYADLFGSRAADTE
                     DSGNLAGVKTPAIDSLISHMVGARTHPDFLAACRALERVVSHSHYLLPQWYSPVHRLA
                     YNAWRLDKPAVVP"
     gene            1774..2577
                     /gene="fabI"
                     /locus_tag="PKN51_08275"
     CDS             1774..2577
                     /gene="fabI"
                     /locus_tag="PKN51_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNT85134.1"
                     /translation="MTTPTGFLTGKKLLITGVLSNRSIAYGIARACRAQGAELAFSYV
                     GERFKDRITEFAAEFDSRLVFDCDVGSDEQIERMFRDLAQVWPRFDGFVHAIGFAPRE
                     AIAGNFFDGLSRENFRVAHDISAYSFPGMAKAALPYLSDKAALLTLSYLGALRSIPNY
                     NTMGLAKASLEASVRYMAEALGGRGVRVNGISAGPIKTLAASGVKDFGKLLGYVAQAA
                     PLRRNVTIDDVGNVAAFLMSDLASGVTAEITYVDGGFSQTAGLSADMVG"
     gene            2665..3510
                     /locus_tag="PKN51_08280"
     CDS             2665..3510
                     /locus_tag="PKN51_08280"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HNT85135.1"
                     /translation="MLLGALALGTRAWAGPADQAPALLLAQPWQPGTDLTDWWVSEKY
                     DGVRGFWDGRTLRTRGGERIAAPPWFTAGWPATPLDGELWAGRGGFEQVQSTVARQSP
                     DDAAWRRLRYMVFDLPAHPGTFDQRLPALRATVAAMDQPWVQAVPQRRVASQAQLQAL
                     LRQVVAGGGEGLMLHRGSSHYRAGRGDDLRKLKTHDDAEARVIAHLPGRGRHAGRLGA
                     LWVETPDGRRFALGAGFSDAERADPPPVGSWVTYRYRGLHAGSGLPRFASFLRVRADE
                     ARFRR"
     gene            complement(3542..4303)
                     /locus_tag="PKN51_08285"
     CDS             complement(3542..4303)
                     /locus_tag="PKN51_08285"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HNT85136.1"
                     /translation="MPPPGPRFDRLDALRALALLWMTAYHFAFDLNHFGHIRQDFYAD
                     PLWTGQRTAIVSLFLLCAGAGQALAVQAGQSAARFARRWAQVAGCALLVSLGSWWMFP
                     KSWISFGVLHGMALMLLLTRALLRRGALRGRWPWLGGLLLIAAAPLAGAWLRQAGPPA
                     LAQALDGRWLNGLGIVTRKPITEDYVPLLPWLGVMWLGVGAAQWAPLRAWLARPAGAA
                     LRALAPLGRWSLSYYMLHQPVLIGLLLAWAALRAG"
     gene            complement(4328..5596)
                     /locus_tag="PKN51_08290"
     CDS             complement(4328..5596)
                     /locus_tag="PKN51_08290"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HNT85137.1"
                     /translation="MHAPDVPIAMTDPCAANLEPLPAELLLNPAVRRDQAYGAQRAAV
                     LGQAALAEARADITRWPGYAPTPLRQLHALAARLGVAELAYKDEGGRFGLGSFKALGG
                     AYAVARLLQRELSARLGRPVGTEELRGGTLAELARQFTVTCATDGNHGRSVAWGAQLL
                     GCGCVIYLHATVSAGRQAAIEALGAQVVRTPGNYDDAVRQADADARRLGRHVISDTSY
                     PGYMDVPRDVMQGYQLMVDEAVQQLGQRPTHVFVQGGVGGLAAAVCGWFWERDGGARP
                     RLVVVEPERADCLLRSARAGRPVAVTGALDTLMAGLACGEVSLLAWDILASGADAFAS
                     IRDAAAVQAMRLLAAPTGSDAPIVAGESAVAGLAAAIALAQVPAQRAALGIDARSRLL
                     LFGSEGATDPALYAQLVGRTPQQVLAGQVR"
     gene            5815..6417
                     /locus_tag="PKN51_08295"
     CDS             5815..6417
                     /locus_tag="PKN51_08295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT85138.1"
                     /translation="MQSASDAWFDEAYYQRYYHDPRTRVVDAEQMRRLGEFVASYLAH
                     LRLPVRRVLDMGCGIGLWRDHVARLFPGASYLGVEYSEHLCQRYGWSRGSVVDWRADE
                     PFDLVICQGVLAYLNPSDLRRALANLDRLCRGALYLEAVTLEDYERDAIDLELSDPRL
                     YRHRAALYRRGLTPHFRAAGGGLWLSRRAPVPLLALEAPD"
     gene            6452..8440
                     /locus_tag="PKN51_08300"
     CDS             6452..8440
                     /locus_tag="PKN51_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNT85139.1"
                     /translation="MPQPHQIELLAPARDADIGIEAIQHGADAVYIGGPGFGARAGAG
                     NPVADIERLARHAHRFDARIFVTLNTILRDDELEPARRLAWQLYDAGADALIIQDMGL
                     LQLDLPPIQLHASTQTDIRTPAKARFLQDAGLSQIVLARELDLTQIAAIRDVLEPARC
                     GIEFFIHGALCVAYSGQCYISHAHTGRSANRGDCSQACRLPYQVVDAQGRFVAHDAHV
                     LSMKDNNQSLNLGPLIDAGVRSFKIEGRYKDMGYVKNITAHYRRLFDDILEQRPELAR
                     ASSGRCRFGFTPDPLQNFNREFTDYFVHGRQIDVGAFDTPKNPGQPIGHVTRVAGDHF
                     ELQTDDPALTLANGDGLCFWDPLKTLVGAQANRVEALGDGRWRVFPKDPMAQLRELRR
                     GTPVHRNRSMDWVRALERKSAERRIDVWLTLSETADGLSLSAIDEDGHSGAAQVALAW
                     AAPRQPAAAEHALREALARLGETLFAPLGIALALPRPWFVPSSVANALRRDAIAALEA
                     ARAAAWQRLPRAQPVEPPTPYPEDTLSYLANVFNHKARDFYARHGVRVIDAAYESHQE
                     PGEVSLMITKHCVRYSLSLCPKQAKGVVGVKGTIRAEPLQLINGKERLTLRFDCKPCE
                     MHVVGRIKPGVVQRAEREAAEHALRFYRAPPRGARSFE"
     gene            8514..8963
                     /locus_tag="PKN51_08305"
     CDS             8514..8963
                     /locus_tag="PKN51_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT85140.1"
                     /translation="MKILLPVDGTELSLHETRFALRLVREGLRASFVLANVQEPASFY
                     EIVTSQNPGLIESAAQEAGEDLMAPSARLLREAGVSFETAVITGDPAHAMLELIELHG
                     CDMVVMGARALGPIRRMLEGGSTSRRLLDDAPVPVLLVKPPPQPQQD"
     gene            9206..9589
                     /locus_tag="PKN51_08310"
     CDS             9206..9589
                     /locus_tag="PKN51_08310"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNT85141.1"
                     /translation="MGLLGLLLSVAIAGRQLALLAVAQSAPGTVIEMVRMASGRLSAA
                     DAYAPRVRFVLDAVEHEFVSPVGSYPPAFQIGDTVRVLYDPANPRRAMVDSSGQLWAP
                     VAVCAVLGLLLGGLGAWLLRKSRVA"
     gene            9821..10195
                     /locus_tag="PKN51_08315"
     CDS             9821..10195
                     /locus_tag="PKN51_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85142.1"
                     /translation="MECFTHPGSSSVGICKTCGKAVCRTCAIDLGFAVACTQACATEA
                     AAIHEMNQRGKKIYGIGVARPKIPSGVIMWLLFGALFSGFGAFAWFKARDLGWFLLLF
                     GAASFFVAWLGYRRAKDVGLQC"
     gene            complement(10297..11076)
                     /locus_tag="PKN51_08320"
     CDS             complement(10297..11076)
                     /locus_tag="PKN51_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT85143.1"
                     /translation="MNRVVLRAWQFGLLLVLLLLWHFASQGSDTVAFFFGKPLEVAQR
                     LWRWFVTDADIYRHLWVTLLETLLAFVIGTLAGLAVGLWLALSPFASAVLDPYLKAAN
                     AMPRVILAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVREVSPVVLANARMLGA
                     NARQLMRHVYVPSAMSWVFSSLHTSVGLAFVGAVVGEYLGSASGVGYLILQAEGSFDI
                     NTVFAGIVLLTVFALVLDGLVGLAERHLMKWQPSSGQTEKL"
     gene            complement(11073..11918)
                     /locus_tag="PKN51_08325"
     CDS             complement(11073..11918)
                     /locus_tag="PKN51_08325"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85144.1"
                     /translation="MTPSSAAALDLQDITCIFTSRDAPGQRYTAVQGASLAVAPGEFV
                     SVVGPTGCGKSTLLNVGAGLLAPSGGQVQVFGQPLAGLNRRAGYMFQTESLMPWKTAL
                     DNVVAGLVFRGTDATEADRLGRAWLARVGLAGFEDRYPHQLSGGMRKRVAMAQTLILD
                     PDIILMDEPFSALDVQTRQLMENELLDLWAAPSASGSAQGRAVLFITHDLDEAIALSD
                     RVVVLSAGPATRPIGEFAIDLPRPRNVTEIRNAPRFVELHAQIWAVLRDEVLKGYDQQ
                     KRKAA"
     gene            complement(12019..13047)
                     /locus_tag="PKN51_08330"
     CDS             complement(12019..13047)
                     /locus_tag="PKN51_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT85145.1"
                     /translation="MRRTVLKTLLAAPAAVALPRLAFAQGKPEKSKLTIAVGGKNLFY
                     YLPLTIAEQLGYFKDEGLQVEIPDFAGGAKALQALVGGSADVVSGAYEHTINMQAKNQ
                     PIMSFVLQGRAPQIVLAVSTRNMPGYKSIADLKGKKIGVTAPGSSTAMMASYVLAKAG
                     LKASDVSFIGVGASSGAISAVKSGQVDAIANLDPVITMLQRDNLIKVVADTRTLKDTQ
                     SVYGGPMPAACLYTPVKFVQDNPGTTQALANAMVRALRWLQKAGPSDIVKTVPDSYLL
                     GDRALYLAAWERVREAISPDGLMPEAGPATALRMLQTFEDSLKGKPIDLGKTYTNNFA
                     RKAAAKYA"
     gene            complement(13177..17235)
                     /gene="purL"
                     /locus_tag="PKN51_08335"
                     /gene_synonym="purI"
     CDS             complement(13177..17235)
                     /gene="purL"
                     /locus_tag="PKN51_08335"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNT85146.1"
                     /translation="MTLHIHFFDGGNALSDFRLRQLLPRLQAVAPAVEGVAARHVHLA
                     GFDAAPAAAELERLAALLDGGDAPAGEWPQGEGLVVTPRLGTVSPWASKATDIARNCG
                     LALHRLERLVEYRIWLKPALIKRQQLSQQERTAIAGLLHDRMTESVLPDREAARALLS
                     TLAPEPLAQVDVLGGGRAALERANRDWGLALAADEIDYLLDAFTRLGRNPTDVELMMF
                     AQANSEHCRHKIFNAQFSIDGVAQERSLFAMIRNTHQASPQHTVVAYSDNAAVMEGGE
                     VQAFQVKSPFEADQASAGSYQKQSMRRHVLMKVETHNHPTAISPFPGASTGAGGEIRD
                     EGATGRGAQPKAGLTGFSVSKLWDSPHGRPEHMASALQIMTEGPLGGAAFNNEFGRPN
                     LLGYFREYELNVGAPGHQVLRGYHKPIMIAGGLGHIDARLTHKLEFPAGSLLIQLGGP
                     GMRIGMGGGAASSLASGANAAQLDFDSVQRGNPEIQRRAQEVITQCQALGAANPILAI
                     HDVGAGGLSNAFPELVNDAGRGARFDLRAVPLEESGLAPKEIWCNESQERYVLAIAPE
                     SLPLFEQLCARERCPLAVVGVATDERELIVADDDRDPAPVRMPMEVLLGKPPKMQRDV
                     QRVQRQFTPLQLDELKLQDAVIEVLSHPTVASKRFLVTIGDRTVGGLTHRDQMVGPWQ
                     VPVADCAVTLADYAGFAGEAMSMGERTPLAALDAPASARMAVAEAITNLLAAPIELPR
                     VKLSANWMAACGEPGEDADLYAAVQAVGMALCPQLGIAIPVGKDSLSMRTQWQDADGH
                     ARKVVSPVSLIASAFVSLADVRGTLTPQLDRHEPDSSLILIDLGHGRHRLAGSVLAQT
                     LGQSGCPQVDGVPDLDDPQDLKALVAAINQLRAEGRILAYHDRSDGGLLACVAEMAFA
                     GQVGVALNIDLLVNEGDGISDSRMESGEGKDWARQVSGRRDLLTLKALFAEELGVVLQ
                     VATRERDAVLQVLRAHGLARLSHVIGKTRPEASPIAKGKGCLSVWRDTREVFSASLHD
                     LQQVWDSVSWRIARERDHPDCADAEHAAAGAPGDPGLHLALSEEFKQKLALAQSEQAP
                     AATKNIVIGGARPKVAVLREQGVNSHVEMAWAFSEAGFQAHDVHMTDLQSGRVRLADF
                     RGLVVCGGFSYGDTLGAGVGWARSITFHAGLSAQFQAFFDRRDTFGLGVCNGCQMFAE
                     LADIIPGAQHWPRFTTNRSERFEARLSMVEVLDSPSILLAGMAGSRLPIAVSHGEGYA
                     NFSRRGDAGQVIAAMRFVDHHGQPTERYPFNPNGSLGGLTAVTTADGRFTAMMPHPER
                     VFRNIQMSWTDGDRSDFSPWMQIWRNARRWVG"
BASE COUNT         2552 a         6194 c         6099 g         2533 t
ORIGIN      
        1 ggcaccacgg ccggcttgtc cagccgccag gcgttgtagg ccagccggtg cacgggtgaa
       61 taccactgcg gcagcaggta gtggctgtgg ctgaccacgc gctccagcgc gcggcaggcg
      121 gccagaaagt cggggtgcgt gcgcgcgccc accatgtggc tgatcaggct gtcgatggcg
      181 ggggtcttca cgccagccag gttgcctgag tcctcggtgt cggcggcgcg gctgccgaac
      241 aggtcggcgt attcctgccc cgggttgagc gtgccctggt gggcgatggt ggtgatgtcg
      301 aagtcgaact tctgcaagcg ctgctggtag agggcgaagt cgaccgggcg gaagttcagg
      361 cggatgccca gcttttcgag gttgcgcatc cagggcgtga ccacgcgcgc gccgccttcg
      421 ttgctgtcca ggtactccag caccaggggc tggccctggg cgttggtcag cacgccgccc
      481 tgcacgcgcc agccggcctc ggccagcagg cgctgggcct ggcgcaggtt ctcgcgcagg
      541 ccgttgtcgc tgccgtcggt gcgcggcggc tcgaaggccg ggccgaaggc cgccggcggg
      601 atgtcggcgc gaaacggggt catcagcgcc agctgggcct cgtcgggcat gccgctggcg
      661 gcgcattcgg tgttgccgaa gatgccgcgc acccgcatgt acgactggta gaacatctgc
      721 cggttcatcc attcgtagtc gatcgccagg cccagcgcct ggcgcacgcg ccggtcgtgc
      781 agcagcggcc ggcggctgtt gagcacgtag ctctgaaagc cggtcggcag gcggtggcga
      841 aattcgccct tgaccagctc gccgctgcgg aacttcggcc cgttgacgcg ccgcgcccag
      901 tcgccggccg aataaaacgc catgaggtcg aattcgcccg ccttcagcgc ctccagccgg
      961 gcggtgttgt cgcggtagat cctgacctgc acgcggtcga agttgccggt gccgcggcgc
     1021 acgttcaggc cctgcgccca gtagctggcg tcgcgcacgt aggtgatgtc cttgccgaac
     1081 ttcaccgggc cgatgcggta ggggccgctg ccgatgggct cgtccatcac cacctggtcg
     1141 aagggcttgc cctgccccca tttgtggctg aacaccggca gaccacccac cgtcagcggc
     1201 agctcgcggt tgggctgccg gaagcgatag cgcacggtgc gctcgtccag cacgtccacc
     1261 cccgccacct cggccagcag cgtcttgtag gcgggcgaag tgtgcgggcc gatcagggtc
     1321 tgaaagctgt gctgcacgtc gcgcgccagc accgggtcgc cgttgtgaaa gcgcgcctgg
     1381 gcgcgcaggc ggaaggtggc cgacaggcgg tcgggcgcca cgctcacgtc ttcggccagc
     1441 aggccgtagg cagcgccggc ttcgtccagc gagccggcca gcaggctgtc gaacagcaga
     1501 tcggccaggt aggtcggcgc gctgcctttg atggtgaagg ggttgtactt gtcgaaggtg
     1561 gaggcgcgcg aattgcccac cagacggatt tcgccgccct tgggcgcggc cgggttgacg
     1621 tagtcgaaat gggcaaagcc gggcgggtac ttcagctcgc cccacagcgc gtaaccgtgg
     1681 gcggcccaga cgggcagact gaaggccgcc agcagcacgg cggggaatag gcgcatgcga
     1741 caattctgcc gctgattttc gaggagctag cgcatgacca cccccaccgg ctttctgacg
     1801 ggcaagaaac tgctgatcac cggcgtgctg tccaaccggt ccatcgccta cggcattgcc
     1861 cgcgcctgcc gcgcacaggg ggccgagctg gccttcagct acgtgggcga gcgcttcaag
     1921 gaccgcatca ccgaattcgc cgccgagttc gactccaggc tggtgttcga ctgcgacgtc
     1981 ggcagcgacg agcagatcga gcgcatgttc cgcgacctgg cccaggtgtg gccgaggttc
     2041 gacggcttcg tgcacgccat cggctttgcg ccgcgcgagg cgattgccgg caacttcttc
     2101 gacggtctgt cgcgcgaaaa cttccgcgtc gcgcacgaca tcagtgccta cagcttcccc
     2161 ggcatggcca aggccgccct gccctacctg agcgacaagg cggcgctgct gacgctgtcc
     2221 tacctgggcg cgctgcgcag catccccaac tacaacacca tgggcctggc caaggccagc
     2281 ctggaagcca gcgtgcgcta catggccgaa gccctgggcg gccgcggcgt gcgcgtcaac
     2341 ggcatcagcg ccggcccgat caagacgctg gcggccagcg gcgtcaagga cttcggcaag
     2401 ctgctgggct acgtggcgca ggccgcgccg ctgcgccgca acgtcaccat cgacgacgtc
     2461 ggcaacgtcg ccgccttcct gatgagcgac ctggccagcg gcgtgacggc ggaaatcacc
     2521 tacgtcgacg gcggcttcag ccagaccgca gggctgtcgg ccgacatggt cggctgagcc
     2581 catcggccgc aagccaaatc agcctctggc gcttgtctgg cgagcgccag aagctattga
     2641 tttgatagtg tgaagcgacg cactctgctg ctcggcgccc tggccctggg cacgcgcgca
     2701 tgggccggtc cggccgacca ggcgccggcg ctgctgctgg cccagccgtg gcagcccggc
     2761 accgacctga ccgactggtg ggtgagcgag aagtacgacg gcgtgcgcgg cttctgggac
     2821 ggccgcacgc tgcgcacgcg cggcggcgag cgcatcgccg ccccgccctg gttcaccgcc
     2881 ggctggcccg cgacgccgct ggacggcgag ctgtgggccg ggcgcggcgg cttcgagcag
     2941 gtgcagtcca ccgtggcgcg ccagagcccc gacgacgcgg cctggcgccg cctgcgctac
     3001 atggtgttcg acctgccggc ccaccccggc accttcgatc agcgcctgcc cgccctgcgc
     3061 gccaccgtcg ccgccatgga ccagccctgg gtgcaggccg tgccgcaacg gcgcgtggcc
     3121 agccaggcgc agttgcaggc gttgctgcgc caggtggtgg cgggcggcgg cgaaggcctg
     3181 atgctgcacc gcggcagctc gcactaccgc gccgggcgcg gcgacgacct gcgcaagctg
     3241 aagacgcacg acgacgccga ggcgcgcgtc atcgcccacc tgcccgggcg cggccgccac
     3301 gccggccggc tgggcgcgct gtgggtcgaa acgcccgatg gccggcgctt tgcgctgggc
     3361 gccggcttca gcgacgccga gcgcgccgac ccgccgcccg tgggcagctg ggtcacgtac
     3421 cgctaccgcg gcctgcatgc gggcagcggc ctgccgcgct ttgccagctt tctgcgcgtg
     3481 cgcgccgacg aggcgcgctt tcgccgctga gcggggccgc gccggcgcgc accgccgggc
     3541 gtcagccagc ccgcagcgcc gcccaggcca gcagcagccc gatcagcacc ggctggtgca
     3601 gcatgtagta gctcaggctc cagcgcccca gcggcgccag cgcgcgcagc gcggcgccgg
     3661 ccgggcgcgc cagccaggcg cgcagcggcg cccactgggc cgcgcccacg cccagccaca
     3721 tcacgcccag ccagggcagc agcggcacgt agtcctcggt gatcggcttg cgcgtgacga
     3781 tgcccagccc gttcagccag cggccatcga gcgcctgcgc cagcgccggc ggcccggcct
     3841 ggcgcagcca ggccccggcc agcggcgcgg cggcgatcag cagcaggccg cccagccacg
     3901 gccagcggcc gcgcagcgcg ccacgccgca gcagcgcgcg cgtcagcagc agcatcagcg
     3961 ccatgccgtg cagcacgccg aagctgatcc agctcttggg gaacatccac cacgacccca
     4021 gcgacaccag cagcgcgcag cccgccacct gggcccagcg gcgcgcaaag cgggccgcgc
     4081 tctggccggc ttgcaccgcc agcgcctgcc cggcgccggc gcacagcagg aacaggctga
     4141 cgatggcggt gcgctggccg gtccacagcg gatcggcgta gaagtcctgg cggatgtgac
     4201 cgaagtggtt gaggtcgaag gcgaagtggt aggccgtcat ccacagcagc gccagcgcgc
     4261 gcagcgcgtc caggcggtcg aagcgggggc cggggggcgg catgggcgat gcgcgggatg
     4321 cgcggggtca gcggacctgc ccggccagca cctgctgcgg cgtgcgcccc accagctgcg
     4381 cgtagagggc cgggtcggtg gcgccttcgc tgccgaacaa cagcaggcgc gagcgggcgt
     4441 cgatgcccag cgccgcgcgc tgcgccggca cctgcgccag cgcgatggcg gcggccagcc
     4501 cggccaccgc cgattcgccg gcgacgatgg gcgcgtcgct ccccgtcggc gcggccagca
     4561 ggcgcatggc ctgcacggcg gcggcgtcgc ggatgctggc aaaggcgtcg gcgccgctgg
     4621 ccaggatgtc ccaggccagc agcgacactt cgccgcaggc caggcccgcc atcagcgtgt
     4681 cgagcgcgcc cgtcaccgcc accggccggc cggcgcgggc gctgcgcagc aggcagtcgg
     4741 cccgttcggg ttcgaccacc accaggcgcg ggcgggcacc accgtcgcgc tcccagaacc
     4801 agccgcacac ggccgccgcc agcccgccca cgccgccctg cacgaacacg tgggtcggcc
     4861 gctggccgag ctgctgcacg gcttcgtcca ccatcagctg atagccctgc atgacgtcgc
     4921 gcggcacatc catgtagccg gggtaggagg tgtcggaaat cacatggcgc cccagccgcc
     4981 gcgcgtcggc gtcggcctgg cgcacggcgt cgtcgtagtt gccgggtgtg cgcaccacct
     5041 gggcgcccaa ggcttcgatg gcggcctggc gcccggcgct gacggtggcg tggaggtaga
     5101 tcacgcagcc gcagcccagc agctgcgcac cccaggccac cgagcgcccg tggttgccgt
     5161 cggtggcgca ggtgacggtg aactggcgcg ccagctcggc cagcgtgccg ccgcgcagct
     5221 cctcggtgcc gaccggccgg cccagccggg ccgacagctc gcgctgcagc agccgcgcca
     5281 cggcgtaggc gccacccagc gccttgaagc tgcccaggcc aaagcgcccg ccctcgtcct
     5341 tgtaggccag ctcggccacg cccaggcgcg ccgccagcgc gtgcagctgg cgcagcggcg
     5401 tgggcgcgta accgggccag cgcgtgatgt cggcgcgcgc ctcggccagc gccgcctgcc
     5461 ccagcaccgc cgcgcgctgc gcaccgtagg cctgatcgcg ccgcacggcg gggttgagca
     5521 gcagttcggc cggcagcggc tcgaggttgg cggcgcaggg gtcggtcatg gcgatcggaa
     5581 cgtcgggggc atgcatgccg cgagtatcgg tgcaatgcgc ggccgcggcg ccagcacagt
     5641 gtttcgcgct gtcaggtacc tatggcgcct agggtataag cctgacaggc ggcgccagcg
     5701 gccaccggcg ccgcggcggg cctgctggcc agtcatcaaa ttactatcct ttcgatagct
     5761 tctggcgctt tcctgtcggg cgccagagcc caatttcatc aggaatcccg gaacatgcaa
     5821 tcggccagcg acgcatggtt tgacgaggcg tactaccagc gctactacca cgacccgcgc
     5881 acgcgcgtgg tcgatgccga gcagatgcgc cggctgggcg agttcgtcgc cagctacctg
     5941 gcgcacctgc gcctgccggt gcggcgcgtg ctcgacatgg gctgcggcat cggcctgtgg
     6001 cgcgaccacg tggcgcggct gttccccggc gccagctacc tgggcgtgga atacagcgag
     6061 cacctgtgcc agcgctacgg ctggagccgc ggctcggtgg tcgactggcg cgccgacgag
     6121 ccgttcgacc tggtgatctg ccagggcgtg ctggcctacc tgaacccgag cgacctgcgg
     6181 cgcgcgctgg ccaacctgga ccggctgtgc cgcggcgcgc tgtacctgga agcggtgacg
     6241 ctggaagact acgaacgcga cgccatcgac ctggagctga gcgacccgcg cctgtaccgc
     6301 caccgcgcgg cgctgtaccg gcgcggcctg acgccccatt tccgcgccgc cggcggcggc
     6361 ctgtggctga gccggcgcgc gccggtgccg ctgctggcgc tggaagcgcc cgactgaacc
     6421 ggcgcccgcg gcgctgggga taatccgctc catgccccag ccgcaccaga tcgaactgct
     6481 ggcccccgcg cgcgacgccg acatcggcat cgaggccatc cagcacggcg ccgacgccgt
     6541 ctacatcggc gggccgggct ttggcgcgcg cgccggcgcc ggcaacccgg tggccgacat
     6601 cgagcgcctg gcgcgccacg cgcaccgctt cgacgcccgc atcttcgtca ccctcaacac
     6661 catcttgcgc gacgacgagc tggagccggc gcgccgcctg gcctggcagc tgtacgacgc
     6721 cggcgccgac gcactgatca tccaggacat gggcctgctg cagctcgacc tgccgccgat
     6781 ccagctgcac gccagcaccc agaccgacat ccgcacgccg gccaaggcgc gctttctgca
     6841 ggatgcgggg ctgagccaga tcgtgctggc gcgcgagctc gatctgaccc agatcgccgc
     6901 catccgcgac gtgctggagc cggcgcgctg cggcatcgag ttcttcatcc acggcgcgct
     6961 gtgcgtggcc tacagcggcc agtgctacat cagccacgcc cacaccgggc gcagcgccaa
     7021 ccgcggcgac tgctcacagg cctgccgcct gccctatcag gtggtggacg ctcagggccg
     7081 cttcgtcgcc cacgacgcgc acgtgctgag catgaaggac aacaaccagt cgctcaacct
     7141 gggcccgctg atcgacgccg gcgtgcgcag cttcaagatc gaggggcgct acaaggacat
     7201 gggctacgtg aagaacatca ccgcccacta tcgccgcctg ttcgacgaca tcctggagca
     7261 gcgccccgag ctggcgcgcg ccagcagcgg ccgctgccgg ttcggcttca cacccgaccc
     7321 gctgcagaac ttcaatcgcg agttcaccga ctacttcgtg cacggccgcc agatcgacgt
     7381 cggcgccttc gacacgccca agaaccccgg ccagccgatc ggccacgtca cgcgcgtggc
     7441 gggcgatcac ttcgagctgc agaccgacga cccggcgctg accctggcca atggcgacgg
     7501 cctgtgcttc tgggacccgc tgaagaccct ggtgggcgcg caggccaacc gcgtcgaggc
     7561 gctgggcgac ggccgctggc gcgtgttccc caaggacccg atggcccagc tgcgcgagct
     7621 gcgccgcggc acgccggtgc accgcaaccg cagcatggac tgggtgcgcg cgctggagcg
     7681 caagtcggcc gagcgccgca tcgacgtctg gctgaccctg agcgaaacgg ccgacggtct
     7741 gagcctgagc gccatcgatg aagacggcca cagtggcgcc gcccaggtcg cgctggcctg
     7801 ggccgcgccg cgccagccgg cggcggccga gcacgcgctg cgcgaagcgc tggcgcggct
     7861 gggcgaaacc ctgttcgcgc cgctgggcat cgcgctcgcg ctgccgcgcc cctggttcgt
     7921 gccgtccagc gtggccaatg cgctgcggcg cgacgccatc gccgcactcg aagcggcgcg
     7981 cgccgccgcc tggcagcgcc tgccgcgcgc ccaaccggtg gagccgccca cgccctaccc
     8041 cgaagacacg ctcagctacc tggccaacgt gttcaaccac aaggcgcgcg acttctacgc
     8101 gcgccacggc gtgcgcgtca tcgacgccgc ctatgaaagc caccaggaac cgggcgaagt
     8161 cagcctgatg atcaccaagc actgcgtgcg ctacagcctc agcctgtgcc ccaagcaggc
     8221 caagggcgtg gtgggcgtca agggcaccat ccgggccgag ccgctgcagc tcatcaacgg
     8281 caaggagcgc ctgacgctgc gcttcgactg caagccgtgc gagatgcacg tggtcggcag
     8341 gatcaagccc ggcgtggtgc agcgcgccga gcgcgaagcc gccgaacacg ccctgcgctt
     8401 ttaccgcgcg cctccgcggg gggcccggtc attcgaatga aatgggccgc tggcgcgcta
     8461 ccagcaagcg ccagcagcta tcttgtttat agcatctgat cgaggaccca accatgaaga
     8521 tcctgctccc cgtcgacggc accgagctgt ccctgcatga aacccgcttt gcgctgcgtc
     8581 tggtgcgcga aggcctgcgc gccagcttcg tgctggccaa cgtgcaggag ccggcctcgt
     8641 tctacgaaat cgtgacctcg cagaacccgg gcctgatcga aagcgccgcg caggaagccg
     8701 gcgaagacct gatggcgccg tccgcccgcc tgctgcgcga agccggcgtg agcttcgaga
     8761 cggcggtcat caccggcgat ccggcgcacg ccatgctgga gctgatcgag ctgcacggct
     8821 gcgacatggt ggtgatgggc gcgcgcgccc tgggcccgat ccgccgcatg ctcgaaggcg
     8881 gctccacctc gcgccggctg ctggacgacg cgccggtgcc cgtgctgctg gtcaaaccgc
     8941 cgccccaacc gcagcaggac tgaccgcgac cgaccggtgg agcgcgccgg cgctgcgctg
     9001 cggggctcgc gggctcgact cgaacgccgc agtcgatcag ccagccaccc aggcgaccgc
     9061 aggcggtcga cagagtcagg caagcagctt gccgttgcgc caccatcggc actcgacccc
     9121 atgttgacca tccatgacgg ctcggactag ccccctacca gaagaggcac ggcgtcgacg
     9181 ccgatggggc cggttcctgc tggcggtggg actgctcggg ctgcttctga gcgtggccat
     9241 cgccgggcgg caactggccc tgctggctgt cgcccagagc gcgccgggaa cggtgatcga
     9301 gatggtgcgc atggcttcgg gccgcctctc tgcagcggat gcctacgccc cgcgcgtgcg
     9361 cttcgtcctc gatgccgtcg agcacgagtt cgtgtccccc gtcggcagtt accctcccgc
     9421 cttccagatc ggcgacacgg ttcgcgttct gtatgacccg gccaacccgc ggcgggccat
     9481 ggtcgacagc tccggtcaac tctgggcgcc ggtggcggtg tgcgcggtgc tgggcttgct
     9541 cctcggtggc ctcggagcct ggctgctgcg caagtcgcgc gtagcctgag tctctttggc
     9601 ttcggaacct tgacattgga accgttcacc cagagcctgt cgaagggctt cgacaagctc
     9661 agcccgaacg gattttgaga cttcaaagga ccggatcaat aggccgaacc catagggtgg
     9721 cgccgaccgc taacatgtcc gcttttggcc cgctgcggat tcgggcgctt cggtgctccg
     9781 gtgccgtgcg atcccacccg ggcgtcagga gtgatttcca atggaatgct ttacccatcc
     9841 gggttccagt tcagtgggaa tatgcaagac ctgcggcaag gcggtgtgcc gcacctgcgc
     9901 catcgatctg gggttcgccg tcgcctgtac ccaggcatgc gccaccgagg cagccgccat
     9961 ccacgaaatg aaccagcgcg gcaagaagat ctacggcatc ggggttgccc gtccgaagat
    10021 ccccagcggg gtgatcatgt ggctgttgtt tggcgccctg ttcagcggct tcggtgcctt
    10081 tgcgtggttc aaagcgcgag acctcgggtg gttccttctg ctgtttggtg ccgcttcctt
    10141 ctttgtggca tggctgggct accgccgcgc caaggatgtc gggctccagt gctagcgcca
    10201 ccgggcgcac atcccggcca gcagaggctc cggcatccac cgccacccct caccccgccc
    10261 tctgcccaga ggggccaggg cagcaagccg cagcgctcac agcttttcgg tctgcccgct
    10321 gctcggctgc cacttcatca ggtgccgctc ggccagaccg accaggccgt cgagcaccag
    10381 ggcaaacacc gtcagcagca cgatgccggc gaacacggtg ttgatgtcga acgaaccttc
    10441 ggcctgcagg atcaggtaac ccacgccgct ggccgagccc aggtattcgc ccaccaccgc
    10501 ccccacgaag gccagtccca ccgacgtgtg caggctcgaa aacacccagc tcatggcgct
    10561 gggcacgtac acgtggcgca tcagctggcg cgcgttggcg cccagcatgc gcgcgttggc
    10621 cagcaccacc gggctgacct cgcgcacgcc ctggtagacg ttgaagaaca cgatgaagaa
    10681 caccagcgtc accgccagcg ccaccttgga ccagatgccc agcccgaacc acatggcgaa
    10741 gatcggcgcc aggatcacgc gcggcatggc gttggcggcc ttcaggtacg ggtccagcac
    10801 cgccgacgcg aacggcgaca gcgccagcca caggcccacc gccagcccgg ccagggtgcc
    10861 gatcacgaag gccagcagtg tttccagcag cgtcacccac aggtggcggt agatgtcggc
    10921 gtcggtcacg aaccagcgcc acaggcgctg cgccacttcc agcggcttgc cgaagaagaa
    10981 ggccacggtg tccgagccct gcgaggcaaa gtgccacagc agcagcagca ccagcagcag
    11041 cccgaactgc caggcgcgca gcaccacgcg gttcatgcgg ccttcctttt ttgctggtcg
    11101 tagcccttga gcacttcgtc gcgcagcacg gcccagatct gcgcgtgcag ctcgacgaag
    11161 cgcggcgcgt tgcggatttc ggtcacgttg cgcgggcgcg gcaggtcgat ggcgaattcg
    11221 ccgatcggcc gcgtggccgg gccggccgac agcaccacca cgcggtcgga cagggcgatc
    11281 gcctcgtcca ggtcgtgcgt gatgaacagc accgcccgcc cctgcgcgct gccgctggcc
    11341 gagggcgcgg cccacagatc gagcaactcg ttttccatca gctggcgggt ctgcacgtcg
    11401 agcgccgaaa acggctcgtc catcaggatg atgtcggggt cgaggatcag cgtctgcgcc
    11461 atcgccacgc gcttgcgcat gccgcccgac aactggtgcg ggtagcggtc ctcgaagccg
    11521 gccaggccca cgcgcgccag ccaggcgcgg cccaggcggt cggcctcggt ggcgtcggtg
    11581 ccgcgaaaca ccagcccggc caccacgttg tccagcgccg tcttccaggg catcagcgac
    11641 tcggtctgga acatgtagcc ggcacgccgg ttcagccccg ccagcggctg gccgaacacc
    11701 tgcacctgcc caccggatgg cgccagcagc ccggcgccca cgttcagcaa cgtgctcttg
    11761 ccgcagccag tgggccccac caccgacacg aattcacccg gcgccaccgc caggctggcg
    11821 ccctgcaccg ccgtgtagcg ctgaccgggg gcgtcgcggc tggtgaagat gcaggtgatg
    11881 tcctgcaggt ccagcgcggc ggcggaagaa ggtgtcataa gcaaaacaag gcgctggctc
    11941 aggcagggca agcgccggca gctacgaaat tgatagtaac tcggccccac cccggggccg
    12001 tgccggccgc cggcgtggtc aggcgtactt ggccgccgcc ttgcgggcga agttgttggt
    12061 gtaggtcttg cccaggtcga tgggcttgcc cttcaggctg tcctcgaagg tctgcagcat
    12121 gcgcagcgcg gtggccgggc cggcctcggg catcaggccg tcgggcgaga tggcttcgcg
    12181 cacgcgctcc caggccgcca ggtacagcgc gcgatcgccc agcaggtacg agtcgggcac
    12241 ggtcttgacg atgtccgacg ggccggcctt ctgcagccag cgcagcgcgc gcaccatggc
    12301 gttggccagc gcctgcgtgg tgccggggtt gtcctgcacg aacttcaccg gcgtgtacag
    12361 gcaggccgcc ggcatcgggc cgccgtagac cgactgggtg tccttgagcg tgcgcgtgtc
    12421 ggccaccacc ttgatcaggt tgtcgcgctg cagcatggtg atcaccgggt cgaggttggc
    12481 gatggcatcg acctgccccg acttgaccgc cgagatggcg ccgctggacg cgcccacgcc
    12541 gatgaaggac acgtcgctcg ccttcagccc cgccttggcc agcacgtagc tggccatcat
    12601 ggcggtggac gaaccgggcg ccgtgacgcc gattttcttg cccttcaggt cggcaatcga
    12661 cttgtagccc ggcatgttcc tggtcgacac cgccagcacg atctgcggcg cccggccctg
    12721 cagcacgaag gacatgatgg gctggttctt ggcctgcatg ttgatggtgt gctcgtaagc
    12781 gcccgacacc acgtcggcgc tgccgcccac cagcgcctgc aaggccttgg cgccgccggc
    12841 gaagtcggga atctcgacct gcaggccctc gtccttgaag taacccagct gctcggcaat
    12901 ggtcagcggc aggtaataga acaggttctt gccgcccacg gcgatggtca gcttggactt
    12961 ctcgggtttg ccctgggcaa aagccaggcg cggcagcgcc acggcggccg gcgcggccag
    13021 cagggtcttc agaacggtac ggcgcatgaa tcggtctccc ggggtgacaa caaaggggca
    13081 aatgtagtgc atgtcaccgc atggcgacgg cgcccgcccc cgagggagcg cgggcaccgg
    13141 cgcgccacgg cggccgggtc gaccgacctg cggcgctcac cccacccagc gccgcgcatt
    13201 gcgccagatc tgcatccagg ggctgaagtc gctgcggtcg ccgtcggtcc agctcatctg
    13261 gatgttgcgg aacacgcgct cggggtgcgg catcatggcg gtgaagcggc cgtcggcggt
    13321 ggtgacggcg gtcaggccgc cgaggctgcc gttggggttg aacgggtagc gctcggtggg
    13381 ctggccgtgg tggtcgacga agcgcatggc ggcgatcacc tggccggcgt cgccgcggcg
    13441 gctgaagttg gcgtagccct cgccgtgcga gacggcgatc ggcagccggc tgccggccat
    13501 gccggccagc aggatgctgg ggctgtccag cacttcgacc atcgacagcc gcgcctcgaa
    13561 gcgttcgctg cggttggtgg tgaagcgcgg ccagtgctgg gcgccgggga tgatgtcggc
    13621 cagctcggcg aacatctggc agccgttgca cacgcccagc ccgaaggtgt cgcggcggtc
    13681 gaagaaggcc tgaaactgcg ccgacagccc ggcgtggaag gtgatggagc gcgcccagcc
    13741 gacgcccgcg cccagcgtgt cgccgtagct gaagccgcca cacaccacca ggccgcggaa
    13801 gtcggccagc cgcacgcgac cgctttgcag gtcggtcatg tgcacgtcgt gggcctgaaa
    13861 gccggcttcg ctgaaggccc aggccatttc gacgtgcgag ttgacgccct gctcgcgcag
    13921 caccgccacc ttcggccggg caccgccgat gactatgttt tttgtagctg ccggcgcttg
    13981 ttccgactgc gccagagcca gtttttgttt gaattcttcg gacagggcca gatgcaggcc
    14041 cgggtcgccc ggcgcaccgg cggcggcgtg ttcggcgtcg gcgcagtcgg ggtggtcgcg
    14101 ctcgcgcgcg atgcgccagc tgacgctgtc ccacacctgc tgcagatcgt gcaggctggc
    14161 gctgaacact tcgcgcgtgt cgcgccacac gctcaggcag cccttgccct tggcgatggg
    14221 cgaggcctcg ggccgcgtct tgccgatcac atggctgagc cgcgccaaac cgtgggcgcg
    14281 cagcacctgc agcacggcgt cgcgctcgcg ggtggccacc tgcagcacca cgcccagttc
    14341 ctcggcgaat agggccttga gcgtcagcag gtcgcgccgg ccgctgacct ggcgcgccca
    14401 gtccttgccc tcgcccgatt ccatgcggct gtcgctgatg ccgtcgccct cgttgaccag
    14461 caggtcgatg ttgagcgcca cgccgacctg gccggcaaac gccatttcgg ccacgcaggc
    14521 cagcaggccg ccgtcgctgc ggtcgtggta ggccaggatc cggccttcgg cgcgcagctg
    14581 gttgatggcg gcgaccaggg ccttgaggtc ctgcgggtcg tccaggtcgg gcacgccgtc
    14641 gacctgcggg cagccgctct ggcccagggt ctgcgccagc acgctgccgg ccaggcggtg
    14701 gcggccgtgg cccaggtcga tcaggatcag gctgctgtcg ggctcatggc ggtcgagctg
    14761 gggcgtgagc gtgccgcgca cgtcggccag gctgacaaag gcgctggcga tcaggctgac
    14821 cggcgagacc accttgcgcg cgtgtccgtc ggcgtcctgc cactgggtgc gcatcgacag
    14881 gctgtccttg cccaccggaa tggcgatgcc cagctgcggg cacagcgcca tgccgaccgc
    14941 ctgcacggcg gcgtagaggt cggcgtcctc gcccggctcg ccgcaggcgg ccatccagtt
    15001 ggccgacagc ttgacgcgcg gcagctcgat gggcgccgcc agcaggttgg tgatggcttc
    15061 ggccaccgcc atgcgcgccg acgcgggggc gtcgagcgcg gccagcggcg tgcgctcgcc
    15121 catgctcatg gcctcgccgg cgaagccggc gtagtcggcc agggtgacgg cgcagtcggc
    15181 caccggcacc tgccaggggc cgaccatctg gtcgcggtgc gtcaggccgc cgaccgtgcg
    15241 gtcgccgatg gtgaccagaa agcgcttgct ggccaccgtg gggtggctga gcacctcgat
    15301 cacggcgtcc tgcagcttca gctcatcgag ctgcaggggg gtgaactggc gctgcacgcg
    15361 ctgcacgtcg cgctgcattt tgggtggctt gccgagcagc acctccatcg gcatccgcac
    15421 cggcgccggg tcgcgatcgt cgtcggcgac gatcagctcg cgctcgtcgg tggccacgcc
    15481 caccaccgcc agcgggcagc gttcgcgcgc gcacagctgc tcgaacagcg gcagcgactc
    15541 gggcgcgatg gccagcacgt agcgttcctg gctttcgttg caccagattt ccttgggcgc
    15601 caggccggat tcttccagcg gcacggcgcg cagatcgaag cgcgccccgc gcccggcgtc
    15661 gttgaccaac tccggaaagg cgttggacag cccgcccgcg cccacgtcgt ggatcgccag
    15721 aatggggttg gccgcgccca gcgcctggca ctgggtgatg acttcctgcg cgcggcgctg
    15781 gatttcgggg ttgccgcgct gcaccgaatc gaaatcgagc tgcgccgcgt tggcgccgct
    15841 ggccagcgac gaggcggcgc cgccgcccat gccgatgcgc atgcccggac cgcccagctg
    15901 gatcagcagg ctgccggcgg ggaattcgag cttgtgcgtc agccgcgcgt cgatgtgccc
    15961 caggccgccg gcgatcatga tcggcttgtg gtagccgcgc agcacctggt ggccgggtgc
    16021 ccccacgttc agttcgtact cgcgaaagta gcccagcagg ttgggccggc cgaactcgtt
    16081 gttgaaggcc gccccgccca gcgggccttc ggtcatgatc tgcagcgcgc tggccatgtg
    16141 ctcggggcgc ccgtgcggac tgtcccacag cttgctgacg ctgaagccgg tcagccccgc
    16201 cttgggctgg gcaccgcggc cggtggcgcc ttcgtcgcga atctcgccgc cggcgccggt
    16261 gctggcgcca ggaaacgggc tgatcgccgt ggggtggttg tgggtttcca ccttcatcag
    16321 cacatgccgc cgcatgcttt gtttttgata gctgccggcg cttgcctgat cggcttcgaa
    16381 cggcgatttg acttgaaacg cctggacctc gccgccttcc atcacggcgg cgttgtcgct
    16441 gtaggccacc accgtatgct gcgggctggc ctggtgcgtg ttccggatca tggcgaacag
    16501 gctgcgctcc tgcgccacgc cgtcgatgct gaactgggcg ttgaagatct tgtgccggca
    16561 gtgctcactg ttggcctggg cgaacatcat cagctcgacg tcggtggggt tgcgccccag
    16621 gcgggtgaag gcgtccagca ggtagtcgat ttcgtccgcc gccagcgcca ggccccagtc
    16681 gcggttggcg cgctccagcg ccgcgcggcc gccgcccagc acatcgacct gggccagcgg
    16741 ctcgggcgcc agggtcgaca gcagcgcgcg cgccgcctcg cggtcgggca gcacgctttc
    16801 ggtcatgcgg tcgtgcagca gcccggcgat cgcggtgcgc tcctgctgtg atagctgctg
    16861 gcgcttgatc agagccggtt tgagccagat tcggtattca accaggcgtt ccagccgatg
    16921 cagcgccaga ccgcagttgc gggcgatgtc ggtggccttg ctggcccagg gcgagaccgt
    16981 gcccagccgc ggcgtgacga ccaacccctc gccctgcggc cactcgcccg cgggggcgtc
    17041 gccgccgtcc agcaaggccg ccaggcgctc cagctcggcg gcggcggggg cggcgtcgaa
    17101 gccggccagg tgcacgtgac gcgcggccac gccctccacc gccggtgcca ccgcctgcag
    17161 gcggggcagc agctggcgaa ggcggaagtc actgagggcg ttgccgccgt cgaagaagtg
    17221 gatgtgcagg gtcacgtcgg gaggcctggg tggggcagtg gcgcgcggcg ccgggtcggg
    17281 gcgactggcc gctgactggg gccagacgcg aagccgtcga ttttatccgc cgccccgggc
    17341 gcgacagggc gcgacctgga cggcgcaggc gcgtcgcg
//
LOCUS       DAOQTZ010000059        17113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3812_length_17113_cov_13.536112, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000059 DAOQTZ010000000
VERSION     DAOQTZ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17113
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3812_length_17113_cov_13.536112"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1289)
                     /gene="hemA"
                     /locus_tag="PKN51_08340"
     CDS             complement(3..1289)
                     /gene="hemA"
                     /locus_tag="PKN51_08340"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNT85147.1"
                     /translation="MAVWTLGLNHHTAPLDLRGRFAFALDQLAPALKGLRASHARHPE
                     AAILSTCNRTEVYCASDNAQVDHTLGWLAQAGQVPAEALRQHTYVLQDGQAARHAFRV
                     ASGLDSMVLGEPQILGQMKSAVRAAEDAGALGRTLNQLFQRSFAVAKQVRSSTEIGAH
                     SISMAAAAVRLAGNLFEDLGKIQVLFVGAGEMIELAATHFAARNPRHIAIANRTLERG
                     EKLATRFGGEVMRMADLPERLHEFDAVVSCTASQLPIIGLGAVERALKKRRHRPIFMV
                     DLAVPRDIEPEVKGLDDVYLYTVDDLAQVVHTGQANRQAAVAQAEAIIDAGVQSFMHW
                     LDQRDPAGGVVPLIQQLGAQADEWRAIELARARKRLARGEDLDAVLDALSRGLTQKML
                     HGAMAELNATDPAHREQTSDAIARLFLRGKLPTQSH"
     gene            1362..1637
                     /locus_tag="PKN51_08345"
     CDS             1362..1637
                     /locus_tag="PKN51_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85148.1"
                     /translation="MDLLDLFWHGLGFVAPALVLAPAMVGVNRWLDRKRAPALSWQAQ
                     TAINFGACLAVLVAGLVLTGHDGRMATYAALVAVAAATPWALGAWRR"
     gene            complement(1660..2409)
                     /gene="ugpQ"
                     /locus_tag="PKN51_08350"
     CDS             complement(1660..2409)
                     /gene="ugpQ"
                     /locus_tag="PKN51_08350"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNT85149.1"
                     /translation="MNARKLPAWPYPFWIAHRGAGRLAPENTLAAFRLGAQHGWRMFE
                     CDAKLSSDGVVFLLHDATLERTTDGAGSAGARPWGELAQLDAGSWLGRAWAGEPIPTL
                     ASVARFCLRNGYHLDIEIKPTPGLEAATGHAVAALAARLWQGAAAPPLLTSFQPAALA
                     AAAQTAPQLPRGLLLDALRDGWLEQARQLGCVAVVCQHALWDAGTVGQVRAAGLRALS
                     YTVNDEWAAERLIRLGTDGVVTDRVDLFSPA"
     gene            complement(2508..3530)
                     /gene="ugpC"
                     /locus_tag="PKN51_08355"
     CDS             complement(2508..3530)
                     /gene="ugpC"
                     /locus_tag="PKN51_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNT85150.1"
                     /translation="MSGISFRNVIKRYGKGPKANQVIHGVSAEIRDGEFVVIVGPSGC
                     GKSTLLRMVAGLEEITEGEIAIGERVVNDVEPARRDIAMVFQNYALYPHMSNFDNMAY
                     GLRIQGVPKDEIRARVDKAAQILELGHLLERKPRELSGGQRQRVAMGRAIVRQPQVFL
                     FDEPLSNLDAKLRAQTRLEIEKLHRELGITSLFVTHDQVEAMTLAERMIVMNAGRMEQ
                     FGTPEEVYHRPASTFVASFIGAPPMNLLKGVEGVRDGVLLGVRPEHLRVVDSGGWTLH
                     VETVEMLGAERLIYGHLNGPQGPWTVLRMNETEQPPAPGQQVQVLPLQDRLHGFDEAT
                     GLRLDH"
     gene            complement(3551..4399)
                     /gene="ugpE"
                     /locus_tag="PKN51_08360"
     CDS             complement(3551..4399)
                     /gene="ugpE"
                     /locus_tag="PKN51_08360"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HNT85151.1"
                     /translation="MVERRPGLTLFSHLVLLLGIAVVAFPIYLAFVASTHTRDAIVQV
                     PMPLLPGGHLLDNYGAALFGAKTQGAHVVVGRMMWISFVTAMVIAVGKIAISLLSAFA
                     IVYFRFPFKKIVFWMIFITLMLPVEVRILPTYKVLSDLNMLNTYAGLTVPLIASATAT
                     FLFRQFFLSVPDELVEAARIDGAGPMRFFKDILVPLSQTSIAALFVIQFIYGWNQYLW
                     PLLATTSEDMYPVVIGIKRMIASGDAAVDWNLVMATAVLALVPPAIVVVLMQRWFVKG
                     LVDTEK"
     gene            complement(4436..5317)
                     /gene="ugpA"
                     /locus_tag="PKN51_08365"
     CDS             complement(4436..5317)
                     /gene="ugpA"
                     /locus_tag="PKN51_08365"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HNT85152.1"
                     /translation="MEKRALFRSGWLPWLLIAPQMAVILVFFFWPASQALMQSVQAED
                     AFGTSTTFVGLDNFVTLFEDPAYVESFKVTAIFSVLVAGCGIALSLVLAIFADRVVRG
                     ALVYKSFLIIPYAVAPAIAGVLWVFMFSPSIGVVSYGLRQMGIDWNHLLNSGQALTLI
                     VVAAVWKQISYNFLFFLAGLQSIPKALIEAAAIDGAGPLRRFFTIQFPLLSPTTFFLL
                     VINVVYAFFDTFAIVHATTEGGPGRDTAILVYKVWHDGFKALDLGGSAAQSVVLMAIV
                     VGLTVIQFRYVEKKVQY"
     gene            complement(5426..6724)
                     /gene="ugpB"
                     /locus_tag="PKN51_08370"
     CDS             complement(5426..6724)
                     /gene="ugpB"
                     /locus_tag="PKN51_08370"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HNT85153.1"
                     /translation="MKFRLAAASLAAALAASPALAQTEIQWWHSMSGPLGEWVGDLAK
                     QFNASQSAYRVVPTYKGQYDESMTAAIAAFRAGNAPDILQVFEVGTATMMASKGAIKP
                     VGEVMTAGGAKFDPSVYISAVAGYYTAPNGQMLSFPFNSSTTVFYYNKDAFKAAGLDP
                     EKAPTTWPEVFAAADKLKAGGHKCPFTTSWTQLESFSAWHNTLFASHNNGFGGTAARL
                     AFNSPLHVRHFENLAKMAKNGTFVYKGRNNAADAAFPSGECAMMTGSSGLYARVAKDA
                     KFKYGISTLPYYPDVKGAPQNTVIGGASLWVMAGKSPEKYKGVARFFDFLSDTKVQSE
                     SHRRTGYLPITTGAFKLTDASGFYKDKPGTDVAVTQMIRKTTDKSRGIRLGNFVQIRT
                     IVDEETEQIWAGKRSAKEALDAAVKRGNEQLARFQKANRN"
     gene            6974..10849
                     /locus_tag="PKN51_08375"
     CDS             6974..10849
                     /locus_tag="PKN51_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HNT85154.1"
                     /translation="MNAPTQPAHLMAAADDEAAPRLREIPYNYTSFSDREIVLRLLGE
                     RAWEIVQTLRSERHTGRSSRMLYEVLGDIWVVRRNPYLQDDLLDNPRRRALLVEALRH
                     RLAEVEKRRHPEADAARDALVGELLTAAHRVVDAFDRSFDKVAELRRRSARVLGRHTH
                     KDNIKFDGLSRVSHVTDATDWRVEFPLVVLTPDSEAEMAALVKGCIELGLTIIPRGGG
                     TGYTGGAIPLDWQSAVINTEKLIDIGRVEPRRLPGLDRDVPTIWTEAGVVTQRVADAA
                     EDAGLVFAVDPTSAEASCIGGNIAMNAGGKKAVLWGTALDNLASWRMVTPAGEWLEVS
                     RIGHNLGKIHDAELARFELVYFDASGKRELRREQLAIPGASFRKAGLGKDVTDKFLSG
                     LPGVQKEGCDGLITSARWVLHRMPEHTRTVCLEFFGHARDAVPSIVEIKDFMFAEQKR
                     SGTLLAGLEHLDDRYLRAVGYSTKSKRGGLPKMVLIGDIAGDDPDAVARAASEVVRIA
                     NSRAGEGFTAVSSEARKKFWADRKKTAAISRHTNAFKINEDVVIPLPRMAEYTDGIER
                     INIELSLSNKIELADELEAFLGRGQLPLGAGAPAPELLQEKVDLALAQVREVRALWQG
                     WLADIDRLFPQLQDHSLRASWKTQIREPLRAIFTGSDFEPLMAALGEIQQRVLKGRVW
                     IALHMHAGDGNVHTNIPVHSDNYAMLQTAHAAVDRVMALARRLDGVISGEHGIGITKL
                     QYLSDDELAPFADYKRRVDPEGRFNRGKLLRNRELPAQPGRRLRADLSNAFTPSFGLM
                     GHESLLLQQSDLGAIADSIKDCLRCGKCKPECSTHVPRANLLYSPRNKILATSLLIEA
                     FLYEEQTRRGISLQHWAEFEDVADHCTVCHRCLAPCPVKIDFGDVTMNMRNLLRKMGK
                     KSVRPGNAAAMFFLNATDPRVINATRAVMTGVAFKAQRAAVDVLRVAGRRQTEHPPAT
                     VGPAPIREQVIHFVNKKLPGGLPTKTARALLDIEDRAYVPVIRDPKGTTSDSEAVFYF
                     PGCGSERLFSQVGLATQAMLWHAGVQTVLPPGYVCCGYPQRGSGQFDKAEKMIVSNRV
                     LLHRVATTLNYLDIKTVVVSCGTCYDQLAGYQFDKIFPGSRIVDIHEYLLEKGITLPA
                     ADQGAYLYHEPCHNPMKQGDSMQTVKALIGPQVIKSERCCGESGTLGVTRPDVSTQVR
                     FRKEEEIRKGEAQLRASGAVADKANVKILTSCPSCLQGLKRYEDDLQNGLLEADYIVI
                     EMARAILGENWLPDYVAKANAGGIERVLV"
     gene            complement(10921..12012)
                     /locus_tag="PKN51_08380"
     CDS             complement(10921..12012)
                     /locus_tag="PKN51_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNT85155.1"
                     /translation="MNTPALQRAVFLLLLAGVTLAFFWIIAPFFGAVFWAVVLTLLFM
                     PLFRRLRAALRGRATLAALATMLICVLIVVVPLGFIVGAMVDEAASFTQRVRSGELNL
                     PAYFQQVLDALPAWLRGLLGRFGLLSMQDVGAKLAAALVQGGQAITGHALAIGQNTLL
                     LLVNLGIMLYLLFFFLRDGRELALLMRRAVPMQPAHASYLLHKFATVVRATVKGTVVV
                     ALVQGLLGGLAFAVLGIHGAVLWGVTMAVLSLVPAVGAALIWAPVAVYLLATGSPVAG
                     IGLAAWGVGVMGMVDNLLRPLLVGKETKLPDFLVLLSTIGGISVFGVNGFVIGPAIAA
                     LFVAAWALFSVAENDASEATPPPSVPPEA"
     gene            12144..12566
                     /locus_tag="PKN51_08385"
     CDS             12144..12566
                     /locus_tag="PKN51_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNT85156.1"
                     /translation="MSATLKPPPESLTVHTLSRMLELRWPDGAQFRIPFELLRVYSPS
                     AEVQGHGPGQEVLQTGKREVEITALAPVGNYAVQPRFSDGHESGIYTWAYLYELGERQ
                     DALWRQYLDRLAAAGLDRDAPMGGKAGGAVSITKTRAS"
     gene            complement(12771..13631)
                     /locus_tag="PKN51_08390"
     CDS             complement(12771..13631)
                     /locus_tag="PKN51_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HNT85157.1"
                     /translation="MNTRQRLRQLAAARRGALVPGAFNALSARVIEDLGFEAVYITGA
                     GVTNMWLGLPDQGFMGLAEIADHTARIRDAVELPLLVDADTGFGNALNVYHTVRTLER
                     AGADGIQLEDQVAPKRCGHFDGKAVISTEEAVSKIKAAVDARRDAELVVMARTDAAAT
                     QGFEAALERAQRFAEAGADILFVEAVTRAEEIRALPQRLPTPQLMNMVIGGKTPIVDA
                     AELGRLGYGLVLYANAALQGALAGMQQALTALRDTGQVLESSGLVTPFAERQRLVGKP
                     RWDALEQCYR"
     gene            complement(13653..14630)
                     /locus_tag="PKN51_08395"
     CDS             complement(13653..14630)
                     /locus_tag="PKN51_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT85158.1"
                     /translation="MTCHRRTLLASAALGALSAALPAAAQDTWPTKTVRLIVPYAAGG
                     FADTRARKIADALGKTLGQPVIVDNRAGAGGVTGTDAIAKATDGHTFGFGSPAPLVTN
                     PMLMKKMPYDAEKAFKPIILIETAPLFLSTAPNQNFKSVKELLDFARANPGKLTYGSS
                     GVGGAHHLSAELLAYQTKTETTHVPYKGGALAANDLMGGHLAFMFEMGYSALPAIQAG
                     KIKALAVSSRQRVAVLPDVPTLNEAGVKGFESYNWLGMIAPASTPDAVIARLNEAVNQ
                     ALKQPDLRQMIEGSAGIVAGGTPAAYGQFLAQERAKWAPVIKAANISLD"
     gene            complement(14768..15982)
                     /gene="prpF"
                     /locus_tag="PKN51_08400"
     CDS             complement(14768..15982)
                     /gene="prpF"
                     /locus_tag="PKN51_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HNT85159.1"
                     /translation="MHPPDACLPPLRIAASYLRGGTSKGVFFCLDDLPAAARQPGPVR
                     DALLRRVLGSPDPYGQQIDGMGGATSSTSKAVILAPASRPDHDVDYLFAQIGVDSGLV
                     DWSGNCGNLSAAVGPFAIARGLLPPARVPRDGVCSVRIWQANIGKTIVAQVPMAGGQV
                     QEAGDFELDGVAFAAAEIGLSFLDPAEGGAGAGGLFPTGRLVDELDVPGIGRLPATLI
                     NAGIPTVFVNARDVGCSGTERQADLNGDAQALARFEAIRAQGALKMGLIATLTEAAQR
                     QHTPKIAFVAPPADYRAASGRQVRAADIDLLVRALSMGRLHHAMMGTAAVAIATAAAI
                     AGTLVHAAAGGGARPTVRFGHPSGTLRVGAQAVQSATGDWQVREVRMSRSARVLMEGW
                     VRVPAEVLGAGA"
     gene            complement(16050..>17113)
                     /locus_tag="PKN51_08405"
     CDS             complement(16050..>17113)
                     /locus_tag="PKN51_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNT85160.1"
                     /translation="LPIQHCWPGDVAPLVTWGLTITRGPNKPRQNLGIYRQQVIGRNQ
                     LIMRWLSHRGGALDFAEHCRQHPGQPYPVAVAIGADPATILGAVTPVPDALSEYQFAG
                     LLRGARTEVADALGVPLQVPAQAEIVLEGHIHPDASHKSGWQHALEGPYGDHTGYYNE
                     CAEFPVFTVERITQREDALYHSTYTGKPPDEPAMLGLALNELFVPLLQRAFPEIIDFY
                     LPPEACSYRMAVVRIRKAYAGHARRVMMGVWSHLRQFLYTKFVVVVDEDIDVRDWKEV
                     VWAMTTRMDPVRDTMLIENTPIDYLDFASPVAGLGSKMGLDATNKWPGETQRDWGRPM
                     AMDAEVTQRMNALADDLGL"
BASE COUNT         2718 a         5737 c         6071 g         2587 t
ORIGIN      
        1 gatcaatggg actgcgtggg cagcttgccc cgcagaaaca gccgcgcaat ggcgtcgctg
       61 gtctgctcgc ggtgggccgg atcggtggcg ttcaattcgg ccatggcgcc gtgcagcatc
      121 ttctgcgtca gcccgcggct cagggcgtcg agcacggcgt cgaggtcttc gccgcgcgcc
      181 aggcgcttgc gcgcgcgcgc cagctcgatg gcgcgccatt cgtcggcctg ggcgcccagt
      241 tgctggatca gcggcaccac gccgccggcc gggtcgcgct ggtccagcca gtgcatgaag
      301 ctctgcacgc cggcgtcgat gatggcctcg gcctgggcca cggcggcctg gcggttggcc
      361 tggccggtgt gcaccacctg ggccaggtcg tccacggtgt agaggtagac gtcgtccagg
      421 cccttgactt cgggttcgat gtcgcgcggc accgccagat cgaccatgaa gatggggcgg
      481 tggcgacgct ttttcagcgc ccgctcgacg gcgcccaggc cgatgatggg cagctggctg
      541 gcggtgcagc tgaccacggc gtcgaactcg tgcaggcgct cgggcaggtc ggccatgcgc
      601 atgacttcgc cgccaaagcg ggtggccagc ttttcgccgc gctccagcgt gcggttggcg
      661 atggcgatgt gccgggggtt cctggcggca aagtgggtgg cggccagctc gatcatttcg
      721 cccgcgccga cgaacagcac ctggatcttg cccaggtctt cgaacaggtt gccggccagg
      781 cgcacggcgg cggcggccat gctgatggag tgggcgccga tttcggtcga gctgcgcacc
      841 tgcttggcga cggcaaagct gcgctggaac agctggttga gcgtgcggcc cagggcgccg
      901 gcgtcttcgg cggcgcgcac ggcgcttttc atctggccca ggatctgcgg ctcgcccagc
      961 accatgctgt ccaggccgct ggcgacgcgg aaggcgtggc gcgcggcctg gccgtcctgc
     1021 agcacgtagg tgtgctggcg cagcgcctcg gccggcacct ggccggcctg ggccagccag
     1081 cccagggtgt ggtcgacctg cgcgttgtcg gaggcgcaat agacctcggt gcggttgcag
     1141 gtggacagga tggcggcttc ggggtggcgc gcgtgcgagg cgcgcaggcc cttgagcgcg
     1201 ggcgcaagct ggtcgagcgc gaacgcaaaa cgcccgcgca gatccagcgg cgcggtgtga
     1261 tggttcagtc ccagggtcca gacggccatg gcaccggatt ataaaattcg gtgttgtttt
     1321 cggctggcct ttggctgcag cgcttgactc ggatcaagaa cttggacctg ctcgaccttt
     1381 tctggcatgg gctcggcttt gtggcccctg ccctggtgct ggcgccggcc atggtcgggg
     1441 tgaatcggtg gctggataga aaacgggctc cggcgctttc ctggcaagcg cagacagcta
     1501 tcaattttgg agcttgtctg gcggtgctgg tggcgggtct ggtgctgacg ggccacgatg
     1561 ggcgcatggc cacctacgcg gccctggtgg cggttgccgc cgccacgccg tgggcgctgg
     1621 gcgcctggcg gcgctgagcc tgctggcgcg ggcgccggct caggccgggc tgaacagatc
     1681 gacgcggtcg gtcaccacgc catcggtgcc gagccggatc agccgctcgg cggcccactc
     1741 atcgttgacg gtgtagctga gcgcacgcag gccggcggcc cggacctggc cgacggtgcc
     1801 ggcgtcccac agcgcgtgct ggcacaccac ggccacgcag ccgagctggc gcgcttgctc
     1861 cagccaaccg tcgcgcagcg catccagcag cagaccgcgc ggcagctgcg gcgccgtctg
     1921 cgcggcggcg gccagggcgg cgggctggaa cgaggtcagc agcggcggtg ccgccgcccc
     1981 ctgccacagg cgcgcggcca gcgccgccac ggcgtggccg gtggctgctt ccaggcccgg
     2041 ggtgggcttg atttcgatgt ccaggtggta gccgttgcgc agacagaagc gcgccacgct
     2101 ggcgagggtg ggaatgggct cgccggccca ggcgcgcccc agccagctgc cggcgtcgag
     2161 ctgcgccagc tcgccccagg gccgcgcgcc ggcgctgccg gcgccgtcgg tggtgcgctc
     2221 cagcgtggcg tcgtgcagca gaaacaccac gccgtcgctg gacagcttgg cgtcgcattc
     2281 gaacatgcgc cagccgtgct gcgcgcccag gcggaaggcg gccagggtgt tctcgggcgc
     2341 cagccggccg gcgccgcggt gcgcgatcca gaacgggtag ggccaggcgg ggagcttgcg
     2401 cgcattcatg gtcaaaaatg cctgcagcgc ttgctggtca agcgccaaca gctattgttt
     2461 tgatagtttg ccttgagtct gggcacgagg ggctggttca caccggctca gtggtccagg
     2521 cgcagcccgg tggcttcgtc gaagccgtgc aggcggtctt gcagcggcag cacctgcacc
     2581 tgctggccgg gtgcgggcgg ttgctcggtt tcgttcatgc gcagcacggt ccaggggccc
     2641 tgcgggccgt tcaggtgacc gtagatcagg cgctcggcgc ccagcatttc gacggtttcc
     2701 acgtgcagcg tccagccgcc gctgtcgacc acgcgcaggt gctcgggtcg cacgcccagc
     2761 agcaccccat cgcgcacgcc ttcgacgccc ttgagcaggt tcatgggcgg cgcgccgatg
     2821 aagctggcca cgaaggtgct ggccggacgg tggtagacct cttcgggggt gccgaactgc
     2881 tccatgcggc cggcgttcat gacgatcatg cgctcggcca gggtcatggc ctcgacctgg
     2941 tcgtgggtga cgaacaggct ggtgatgccc agctcgcggt gcagtttctc gatttccagg
     3001 cgcgtctgcg cgcgcagctt ggcgtccagg ttggacagcg gttcgtcgaa caggaacacc
     3061 tgcggctggc gcacgatggc gcggcccatg gccacgcgct ggcgctggcc gcccgacagc
     3121 tcgcgcggct tgcgctccag caggtggccc agctccagga tctgcgccgc cttgtcgacg
     3181 cgggcgcgga tctcgtcctt gggcacgccc tgaatgcgca ggccgtaggc catgttgtcg
     3241 aagttgctca tgtgcgggta gagcgcgtag ttctggaaca ccatggcgat gtcgcgcctg
     3301 gccggctcca cgtcgttgac cacgcgctcg ccgatggcga tctcgccttc ggtgatttcc
     3361 tccagcccgg ccaccatgcg cagcagggtg gatttgccgc agcccgacgg gccgacgatg
     3421 acgacgaatt cgccgtcgcg gatctcggca ctcacgccgt ggatcacctg gttggccttg
     3481 gggcccttgc cgtagcgctt gatgacgttg cgaaaggaga tgccggacat ggggtgcgtg
     3541 cgggggaggg ttatttttcg gtgtcgacca ggcccttgac gaaccagcgc tgcatcagca
     3601 ccaccacgat ggcgggcggc accagcgcca gcacggcggt ggccatcacc aggttccagt
     3661 cgacggcggc gtcgcccgag gcgatcatgc gcttgatgcc gatcaccacc gggtacatgt
     3721 cctcgctggt ggtggccagc agcggccaca ggtactggtt ccagccgtag atgaactgga
     3781 tcacgaacag cgccgcgatc gaggtctgcg acagcggcac cagaatgtcc ttgaagaagc
     3841 gcatcggccc ggcgccgtcg atgcgcgcgg cctccaccag ctcgtcgggc accgacagga
     3901 agaactgccg gaacaggaag gtggcggtgg ccgaggcgat cagcggcacc gtcagaccgg
     3961 cataggtgtt gagcatgttc aggtccgaca gcaccttgta ggtgggcagg atgcgcacct
     4021 ccaccggcag catcagggtg atgaagatca tccagaacac gatcttcttg aacggaaagc
     4081 ggaagtagac gatggcgaac gccgacagca gcgagatggc gatcttgccc accgcgatca
     4141 ccatggcggt gacgaagctg atccacatca tgcgccccac caccacgtgc gcgccctgcg
     4201 tcttggcgcc gaacagcgcg gcgccgtagt tgtcgagcag gtggccgccg ggcagcagcg
     4261 gcatcggcac ctgcacgatg gcgtcgcgcg tgtgtgtgga ggccacgaag gccaggtaga
     4321 tcgggaacgc caccaccgca atgcccagca gcagcaccag gtgcgaaaac agcgtcaggc
     4381 cggggcggcg ttcaaccatg gcggggcctc gcggtggggt gggcggtgtc gggcatcagt
     4441 actgcacctt cttttcgacg tagcggaact ggatcaccgt caggccgacc acgatggcca
     4501 tcagcaccac cgactgcgcg gccgagccgc ccaggtcgag cgccttgaag ccgtcgtgcc
     4561 agaccttgta gaccaggatg gcggtatcgc gtcccggccc gccctcggtg gtggcgtgga
     4621 cgatggcgaa ggtgtcgaag aaggcgtaca ccacgttgat caccagcagg aagaaggtgg
     4681 tgggcgacag cagcggaaac tggatggtga agaagcgccg cagcggcccg gcgccgtcga
     4741 tcgcggcggc ctcgatcagc gccttgggga tggactgcag gccggccaga aagaacagga
     4801 agttgtagga aatctgcttc cacaccgcgg ccaccacgat cagcgtcaac gcctggccgg
     4861 agttcagcag gtggttccag tcgatgccca tctgccgcag gccgtagctg accacgccga
     4921 tgctgggcga gaacatgaac acccacagca cgccggcaat ggccggcgcc acggcgtagg
     4981 gaatgatcag aaagctcttg tagaccagcg cgccgcgcac cacgcggtcg gcaaagatgg
     5041 ccagcaccag cgacagcgcg atgccgcagc cggccaccag caccgagaag atcgccgtca
     5101 ccttgaagga ttcgacgtag gccgggtctt cgaacagggt gacgaagttg tccaggccga
     5161 cgaaggtggt gctggtgccg aaggcgtctt cggcctgcac cgactgcatc agggcctggc
     5221 tggcgggcca gaagaagaac accaggatca ccgccatctg cggcgcgatc agcagccagg
     5281 gcagccagcc ggagcgaaag agggcgcgtt tttccatggg tggcgcgaat gagaaaagcc
     5341 cgccggcggg tgggtcggcg ggcgatcagt ctagcgggtt gcgccgccgg ccggcggcag
     5401 gcgccgtcgg ccggtgcccg gcccttcagt tgcggttggc tttctggaag cgtgccagct
     5461 gctcgttgcc gcgcttgacg gcggcgtcca gcgcttcctt ggcgctgcgt ttgccggccc
     5521 agatctgctc ggtttcctcg tcgacgatgg tgcggatctg cacgaagttg cccaggcgga
     5581 tgccgcgcga tttgtcggtg gtcttgcgga tcatctgcgt caccgcgacg tcggtgccgg
     5641 gcttgtcctt gtagaagccg gaagcgtcgg tcagcttgaa ggcaccggtg gtgatcggca
     5701 ggtagccggt gcgccggtgg ctttcggact gcaccttggt gtcggacagg aagtcgaaga
     5761 agcgcgccac ccccttgtac ttctcggggg acttgccggc catcacccac aggctggcgc
     5821 cgccgatcac ggtgttctgc ggcgcgccct tgacgtcggg gtagtagggc agggtggaga
     5881 tgccgtactt gaacttggcg tccttggcca cgcgcgcgta caggcccgag gagccggtca
     5941 tcatggcgca ttcacccgag gggaaggcgg catcggcggc gttgttgcgg cccttgtaga
     6001 cgaaggtgcc gttcttggcc atcttggcca ggttctcgaa atgccgcaca tgcagcggcg
     6061 agttgaaggc cagccgtgcc gccgtgcccc cgaagccgtt gttgtggctg gcgaacaggg
     6121 tgttgtgcca ggccgagaag ctctccagct gcgtccagct ggtggtgaac gggcacttgt
     6181 ggccgccggc cttgagcttg tcggcggcgg caaacacttc cggccaggtg gtgggggcct
     6241 tttcggggtc cagcccggcg gccttgaagg cgtccttgtt gtaatagaac actgtcgtcg
     6301 agctgttgaa cgggaagctc agcatctgcc cgttgggcgc ggtgtagtag ccggccacgg
     6361 ccgagatgta gacgctgggg tcgaacttgg cgccgccggc ggtcatgacc tcgcccaccg
     6421 gcttgatggc gcccttggag gccatcatgg tggcggtgcc gacctcgaac acctgcagga
     6481 tgtcgggcgc gttgccggcg cggaaggcgg cgatggcggc ggtcatggat tcgtcgtact
     6541 ggcccttgta ggtgggcacc acccggtagg cgctctggct ggcgttgaac tgcttggcca
     6601 ggtcacccac ccattcgccc agcgggccgc tcatcgagtg ccaccactgg atttcggttt
     6661 gcgccagcgc gggcgatgcg gcgagggcgg ccgccaggct ggcggcggca agacggaatt
     6721 tcatgaaacc tccttggaca atggggaaaa tgcggcgcca cagcccctgc gacgcacagc
     6781 accatgatgg gacaggcgga ttgcacgccc atgtcagccg cgtgacaagc gggtttgtac
     6841 gcaagccggg gcccgacctc agaccctgtg tgtgaatgtt gcaccgcagt aaaatcgccg
     6901 gccagccgga cgccgggtgc gccttgtgcg cttgttttgc cgtggcttgt tggcgccgcg
     6961 ctgggcccac cacatgaacg ctccgaccca gcccgcccac ctgatggccg ccgccgatga
     7021 cgaggcggcg ccgcgcctgc gcgagatccc ctacaactac accagctttt ccgaccgcga
     7081 gatcgtgctg cggctgctgg gcgagcgcgc ctgggagatc gtgcagacgc tgcgcagcga
     7141 acgccacacc ggccgctcct cgcgcatgct gtacgaggtg ctgggcgaca tctgggtggt
     7201 gcggcgcaac ccctacctgc aggacgacct gctggacaac ccgcgccgtc gcgcactgct
     7261 ggtcgaagcg ctgcgccacc gcctggcgga ggtcgaaaag cgccgccacc ccgaggcgga
     7321 cgccgcgcgc gacgccctgg tcggcgagct gctgacggcg gcccaccggg tggtcgatgc
     7381 cttcgatcgc tcgttcgaca aggtggccga gctgcgccgc cgcagcgcgc gcgtgctggg
     7441 tcgccacacg cacaaggaca acatcaagtt cgacggcctg tcacgcgtca gccacgtgac
     7501 cgacgccacc gactggcggg tggagtttcc gctggtggtg ctgacgcccg acagcgaggc
     7561 cgagatggcg gcgctggtca agggttgcat cgaactgggc ctgaccatca tcccgcgcgg
     7621 tggcggcacc ggctacaccg gcggcgccat tccgctggac tggcagagcg cggtcatcaa
     7681 caccgaaaag ctgatcgaca tcggccgcgt cgagcccagg cgcctgcccg ggctcgaccg
     7741 cgacgtgccc accatctgga ccgaagccgg cgtggtgacc cagcgcgtgg ccgacgcggc
     7801 cgaagacgcc ggcctggtgt tcgcggtcga cccgaccagc gccgaagcca gctgcatcgg
     7861 cggcaacatc gccatgaacg ccggtggcaa gaaagccgtg ctgtggggca cggcgctgga
     7921 caacctggcg agctggcgca tggtcacgcc cgccggcgaa tggctggaag tgagccgcat
     7981 cggccacaac ctgggcaaga tccacgacgc cgagctggcg cgcttcgagc tggtctattt
     8041 cgacgccagc ggcaagcgtg aactgcgccg cgagcagctg gccatccctg gcgccagctt
     8101 ccgcaaggcc ggcctgggca aggacgtgac cgacaaattc ctcagcggcc tgcccggggt
     8161 gcagaaggag ggctgcgacg gcctgatcac cagcgcgcgc tgggtgctgc accgcatgcc
     8221 cgagcacacc cgcacggtgt gcctggagtt ctttggccac gcgcgcgacg ccgtgcccag
     8281 catcgtcgag atcaaggact tcatgttcgc cgagcagaag cgcagcggca ccctgctggc
     8341 cgggctggag cacctggacg accgctacct gcgcgccgtc ggctacagca ccaagtccaa
     8401 gcgcggcggt ctgcccaaga tggtgctgat tggcgacatc gccggcgacg accccgacgc
     8461 cgtggcgcgc gccgccagcg aggtggtgcg catcgccaac tcgcgcgccg gcgagggctt
     8521 caccgccgtg agcagcgagg cgcgcaagaa gttctgggcc gaccgcaaga agacggcggc
     8581 gatttcccgc cacaccaacg ccttcaagat caacgaggac gtggtgattc cgctgccgcg
     8641 catggccgaa tacaccgacg gcatcgagcg catcaacatc gagctcagcc tgagcaacaa
     8701 gatcgagctg gccgacgagc tggaggcctt tctcggtcgc ggccagctgc cgctgggtgc
     8761 cggcgcgccc gcacccgagc tgctgcagga aaaggtcgac ctcgccctgg cccaggtgcg
     8821 cgaggtgcgc gcgctgtggc agggctggct ggccgacatc gaccggctgt tcccccagct
     8881 gcaggaccac agcctgcgcg ccagctggaa gacccagatt cgcgagccgc tgcgcgccat
     8941 cttcaccggc agcgacttcg agccgctgat ggcggcgctg ggcgagatcc agcagcgcgt
     9001 gctgaagggg cgcgtgtgga tcgccctgca catgcacgcc ggcgacggca acgtgcacac
     9061 caacatcccc gtgcacagcg acaactacgc catgctgcaa accgcgcacg ccgcggtcga
     9121 ccgcgtgatg gcgctggcgc gccgcctgga cggcgtgatc agcggcgagc acggcatcgg
     9181 catcaccaag ctgcagtacc tgagcgacga cgagctggcg ccgtttgccg actacaagcg
     9241 gcgcgtcgat cccgagggcc gtttcaaccg cggcaaattg ctacgaaata gggagctgcc
     9301 ggcgcagcct gggcgccgtc tgagggccga tttgagcaat gccttcacgc ccagcttcgg
     9361 gctcatgggg cacgaatcgc tgctgctgca gcaaagcgac ctgggcgcca tcgccgactc
     9421 gatcaaggac tgcctgcgct gcggcaaatg caagcccgag tgcagcaccc acgtgccgcg
     9481 cgccaacctg ctgtacagcc cgcgcaacaa gatcctggcc acctcgctgc tgatcgaggc
     9541 gtttctgtac gaagagcaga cgcgccgcgg catcagcctg cagcactggg ccgaattcga
     9601 ggacgtggcc gaccactgca cggtgtgcca ccgctgcctg gcaccctgcc cggtgaagat
     9661 cgacttcggc gacgtcacca tgaacatgcg caacctgctg cgcaagatgg gcaagaagag
     9721 cgtgcggccc ggcaacgccg ccgccatgtt cttcctgaac gccaccgatc cgcgcgtcat
     9781 caacgccacc cgtgcggtga tgaccggcgt ggccttcaag gcgcagcgcg ccgccgtcga
     9841 cgtgctgcgc gtggccgggc gccggcagac cgagcatccg cccgccaccg tcggcccggc
     9901 gccgatccgc gagcaggtga tccacttcgt caacaagaag ctgcccggcg gcctgcccac
     9961 caagaccgca cgcgccctgc tcgacatcga agaccgcgcc tacgtgccgg tgatccgcga
    10021 ccccaaaggc accacctccg acagcgaggc ggtgttctac tttccgggct gcggctccga
    10081 gcggctgttc agccaggtcg gcctggccac ccaggccatg ctgtggcatg ccggcgtgca
    10141 gaccgtgctg ccgcccggct acgtgtgctg cggctacccg cagcgcggca gcggccagtt
    10201 cgacaaggcc gagaagatga tcgtcagcaa ccgcgtgctg ctgcaccggg tggccaccac
    10261 gctcaactac ctggacatca agaccgtggt ggtcagctgc ggcacctgct acgaccagct
    10321 ggcgggctac cagttcgaca agatcttccc cggcagccgc atcgtcgaca tccacgaata
    10381 cctgctggaa aagggcatca cgctgccggc ggccgaccag ggcgcctacc tgtaccacga
    10441 gccctgccac aacccgatga agcagggtga ctcgatgcag accgtcaagg cgctgatcgg
    10501 cccgcaggtc atcaagagcg agcgctgctg cggcgagtcg ggcactctgg gtgtgacgcg
    10561 ccccgacgtc tcgacccagg tgcgctttcg caaggaagaa gaaatccgca agggcgaagc
    10621 ccaactgcgc gccagcggcg cggtggccga caaggccaac gtcaagatcc tgaccagctg
    10681 cccgagctgc ctgcagggcc tcaagcgcta cgaagacgac ctgcaaaacg gcctgctgga
    10741 ggccgactac atcgtgatcg agatggcgcg cgccatcctg ggcgaaaact ggctgcccga
    10801 ctatgtggcc aaggccaacg cggggggcat cgagcgggtg ctggtctgac cgccagcgcc
    10861 cacagccgaa cgcagaggac gcgcagaagc cctggaagtg ggcgcccgcg ggggcggcgc
    10921 tcaggcttcg ggcggcactg acgggggcgg cgtggcctcg gaggcgtcgt tctcggccac
    10981 gctgaacagc gcccaggcgg ccacgaacag cgccgcgatc gccgggccga tgacgaagcc
    11041 attgacgccg aacaccgaaa tgccgccgat ggtcgacagc agcaccagga agtccggcag
    11101 cttggtttcc ttgcccacca gcagcgggcg cagcaggttg tcgaccatgc ccatcacgcc
    11161 cacgccccag gccgccaggc cgatgccggc gacgggcgag ccggtggcca gcagatagac
    11221 ggccaccggg gcccagatca gcgccgcgcc gacggccggc accagcgaca gcacggccat
    11281 ggtgacgccc cacagcaccg cgccgtggat gcccagcacc gcaaacgcca gtccgcccag
    11341 caggccctgc accagcgcca ccaccaccgt gcccttgacg gtggcgcgca ccacggtggc
    11401 gaacttgtgc agcaggtagc tggcgtgcgc cggctgcatc ggcacggcgc ggcgcatcag
    11461 cagcgccagc tcgcgcccgt cgcgcaggaa aaagaacagc aggtacagca tgatgcccag
    11521 gttgaccagc agcagcagcg tgttctgccc gatcgccagc gcgtggccgg tgatggcctg
    11581 gccgccctgc accagcgcgg ccgccagctt ggcgcccacg tcctgcatgc tgagcagccc
    11641 gaagcggccc agcagcccgc gcagccaggc cggcagtgcg tccagcacct gctggaaata
    11701 ggccggcagg ttgagctcgc ccgaacgcac gcgctgggtg aagctggccg cctcgtccac
    11761 catggcgccg acgatgaagc ccagcggcac caccacgatc agcacgcaga tcagcatggt
    11821 ggccagcgcc gccagcgtgg cgcgcccgcg cagggcggcg cgcaggcggc gaaacagcgg
    11881 catgaacagc agcgtcagca ccacggccca gaacaccgca ccgaagaacg gcgcgatgat
    11941 ccagaaaaac gccagcgtca ccccggccag cagcagcaga aagacggcgc gttgcaaggc
    12001 gggggtgttc acggcgggtg ggtggcgggg tgggcggtgc gcgcagtata ggagccgacg
    12061 ccggacggcg gccgtgggcg cacaatcgac gcacaatcgc tgccgtcgca gccgaacacg
    12121 cgccgccccg gcgtgcacct gccatgtccg ccacgctcaa accgccgccc gaatcgctca
    12181 ccgtgcacac cttgtcgcgc atgctggagc tgcgctggcc cgacggcgcg cagttccgca
    12241 tcccgttcga gctgctgcgc gtgtactcgc cctcggccga ggtgcagggc catggcccgg
    12301 gccaggaagt gctgcagacc ggcaagcgcg aggtcgagat caccgcgctg gccccggtcg
    12361 gcaactacgc cgtgcagcca cgcttttcgg acgggcacga gagcggcatc tacacctggg
    12421 cctacctgta cgaactgggc gaacgccagg acgcgctgtg gcgccagtac ctcgatcgac
    12481 tggccgccgc cggcctggac cgcgacgccc ccatgggcgg caaggcgggc ggcgcggtgt
    12541 cgatcaccaa gaccagggcg tcctgactgc cgacccaggc gttcgcgggg cggccagacc
    12601 cgccgcgggc gcgcatgggc gccagtggct gattcctgtc atcgccgctt tcgcagggat
    12661 gacggtgggt ttgctcggac atcgcgagcc cgtgacagac aaggcatgtg ctatcaatta
    12721 aatagcttct ggcgctcggc tgacgccagg tgaaggccat ttggccattg tcagcggtag
    12781 cactgctcca gcgcgtccca gcgcggcttg cccaccaggc gctggcgctc ggcaaacggc
    12841 gtcaccaggc cgctggattc gagcacctgg ccggtgtcac gcagcgccgt cagcgcctgc
    12901 tgcatgccgg ccagcgcgcc ctgcagcgcc gcgttggcgt acagcaccag gccgtagccg
    12961 agccgcccca gttcggcggc gtcgacgatc ggcgtcttgc cgccgatcac catgttcatc
    13021 agctgcggcg tcggcaggcg ctgtggcagg gcgcggattt cttcggcgcg ggtgacggct
    13081 tcgacgaaca ggatgtcggc gccggcctcg gcaaagcgct gcgcgcgctc cagcgccgcc
    13141 tcgaaaccct gggtggcggc ggcgtcggtg cgcgccatga ccaccagctc ggcgtcgcgc
    13201 cgggcatcga cggcagcctt gatcttgctg accgcttcct cggtgctgat gaccgccttg
    13261 ccgtcgaaat gcccgcagcg cttgggcgcc acctggtctt ccagctggat gccgtcggcg
    13321 ccggcgcgct ccagcgtgcg caccgtgtgg tagacgttca gcgcgttgcc gaagccggtg
    13381 tcggcatcca ccagcagcgg cagctcgacc gcgtcgcgga tgcgcgccgt gtggtcggcg
    13441 atctcggcca agcccatgaa gccctggtcg ggcaggccca gccacatgtt ggtgacgccg
    13501 gcgccggtga tgtagacggc ctcgaagccc aggtcctcga tcacgcgcgc cgacagcgcg
    13561 ttgaaggcgc ccggcaccag cgcgccgcgc cgcgccgcgg ccagttgtct gagtcgttgt
    13621 cgggtgttca cgccaggtgc cttctttctg gatcagtcga gcgagatgtt ggcggccttg
    13681 atgaccggcg cccacttggc gcgctcctgc gccaggaact ggccataggc cgccggcgtg
    13741 ccgccggcca cgatgccggc gctgccctcg atcatctggc gcagatccgg ctgcttgagc
    13801 gcctggttga cggcttcgtt cagccgggcg atgacggcat cgggcgtgct ggccggcgcg
    13861 atcatgccca gccagttgta ggactcgaag cccttgacgc cggcttcgtt cagcgtcggc
    13921 acgtcgggca gcacggccac gcgctggcgg ctggagacgg ccagcgcctt gatcttgccg
    13981 gcctggatgg ccggcagggc cgagtagccc atctcgaaca tgaaggccag gtggccaccc
    14041 atcaggtcgt tggcggccag cgcgccgccc ttgtagggga catgggtggt ttcggtcttg
    14101 gtctggtagg ccagcaactc ggccgacaga tggtgggcgc cacccacgcc ggaggagccg
    14161 taggtcagct tgccggggtt ggcgcgggcg aagtccagca gttccttgac cgacttgaag
    14221 ttctggttcg gcgcggtgga caggaacagc ggcgcggtct cgatcaggat gatgggcttg
    14281 aacgcctttt cggcgtcgta gggcatcttc ttcatcagca tcgggttggt caccagcggc
    14341 gcgggcgagc cgaagccgaa ggtgtggccg tcggtggcct tggcgatggc atcggtgccg
    14401 gtgacgccgc cggcacccgc ccggttgtcg acgatgaccg gctggcccag ggtcttgccc
    14461 agcgcgtcgg cgatcttgcg cgcgcgcgtg tcggcaaagc cgccggcggc gtagggcacg
    14521 atcaggcgca cggtcttggt gggccaggtg tcctgcgcgg cggcaggcag ggcggcagac
    14581 agcgcgccca gggcggcgct ggcaagcagg gtgcggcggt ggcaggtcat gcaggcgtcc
    14641 ttcggatcga aaagaccatt atgggccgcc gggaccggcc tatttccatg gcgcttcaaa
    14701 ttcaatagct gctggcgcag actggtaaat cgctgcaagc atctttttct tgcaacacag
    14761 cggcgcgtca ggccccggcg cccagcacct cggccggcac gcgcacccag ccttccatca
    14821 gcacgcgggc gctgcggctc atgcgcactt cgcgcacctg ccagtcgccg gtcgcgcttt
    14881 gcaccgcctg cgcgcctacg cgcagcgtgc cggaggggtg accgaagcgc accgtcgggc
    14941 gcgcgccgcc accggcggcg gcatgcacca gggtgcccgc aatggcggcg gcggtggcga
    15001 tggccaccgc cgccgtgccc atcatggcgt ggtgcagccg gcccatcgac agcgcgcgca
    15061 ccagcaggtc gatgtcggcc gcgcgcacct gtcggccact ggcggcgcgg tagtcggccg
    15121 gcggcgccac gaaggcgatc ttgggcgtgt gctggcgctg ggcggcttcg gtcagcgtgg
    15181 cgataaggcc catcttcagg gcaccctggg cgcggatggc ctcgaagcgc gccagcgcct
    15241 gcgcgtcgcc gttgaggtcg gcctgtcgct cggtgccgct gcagccgaca tcgcgggcgt
    15301 tgacgaacac ggtggggatg ccggcgttga tcagcgtggc cggcaggcga ccgatgccgg
    15361 gcacgtccag ctcgtcgacc aggcggccgg tgggaaacag tccacccgcg cctgcgccgc
    15421 cctcggccgg gtcgagaaag ctcagcccga tctcggcggc cgcgaaggcc acgccgtcga
    15481 gctcgaagtc gcccgcttcc tgcacctggc cgccggccat gggcacctgg gcgacgatgg
    15541 tcttgccgat gttggcctgc cagatgcgca cgctgcagac gccgtcgcgc ggcacgcgcg
    15601 cgggcggcag cagaccgcgg gcgatggcga acggccccac ggcggccgac aggttgccgc
    15661 agttgcccga ccagtccacc agcccgctgt cgacgccgat ctgggcaaac aggtagtcga
    15721 cgtcgtggtc gggccggctg gccggggcca ggatcacggc cttggaggtg gacgacgtgg
    15781 cgccgcccat gccgtcgatc tgctgcccgt aggggtcggg actgcccagc acgcgccgca
    15841 gcagcgcgtc gcgcaccgga ccgggctggc gcgccgcggc gggcagatca tccaggcaga
    15901 agaacacgcc cttgctggtg ccgccgcgca ggtagctggc ggcaatccga agcgggggca
    15961 ggcaggcgtc gggcgggtgc atggcgccat ggtacgcgcc aagcgcggcg cacgtgacga
    16021 tggcaagcgg ccgacggccg ttttgagggt tacagcccca ggtcgtcggc cagtgcgttc
    16081 atgcgctggg tcacctcggc atccatggcc atgggccggc cccaatcgcg ctgcgtctcg
    16141 ccgggccatt tgttcgtcgc gtccaggccc atcttgctcc cgaggcccgc cacgggcgag
    16201 gcaaagtcga ggtagtcgat gggggtgttc tcgatgagca tggtgtcgcg caccgggtcc
    16261 atgcgcgtgg tcatggccca gacgacctcc ttccaatcgc gcacgtcgat gtcctcatcg
    16321 acgacgacga cgaacttggt gtagagaaat tggcgcaggt ggctccacac gcccatcatc
    16381 acgcgccgcg cgtggccggc gtaggccttg cggatacgca ccacggccat gcggtagctg
    16441 caggcctcgg gcgggaggta gaagtcgatg atctccggga aggcgcgctg caacagcggc
    16501 acgaacagct cgttgagcgc caggcccagc atggccggct cgtccggcgg cttgccggtg
    16561 taggtgctgt ggtagagcgc gtcctcgcgc tgggtgatgc gctccacggt gaacaccggg
    16621 aactcggcgc attcgttgta gtagcccgtg tggtcgccat acggcccttc gagcgcgtgt
    16681 tgccagccgc tcttgtggct ggcgtcgggg tggatatggc cctcgagcac gatctccgcc
    16741 tgggccggca cttgcaaagg cacgcccagc gcgtcggcca cctcggtgcg cgcgccgcgc
    16801 aagaggccag caaactggta ttcggacaaa gcgtcgggca cgggggtgac cgcgcccagg
    16861 atggtcgctg ggtccgcgcc aatggccacg gccacggggt agggctggcc ggggtgctgg
    16921 cggcaatgct cggcaaagtc cagcgccccg ccgcggtgcg agagccagcg catgatgagc
    16981 tggttcctgc caatcacctg ctggcggtag atgccgaggt tctgccgtgg cttgttcggc
    17041 ccgcgcgtga tggtcaggcc ccaggtgacg agcggcgcca cgtcgccggg ccagcaatgc
    17101 tgtatgggca ggc
//
LOCUS       DAOQTZ010000060        17093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3822_length_17093_cov_13.769339, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000060 DAOQTZ010000000
VERSION     DAOQTZ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3822_length_17093_cov_13.769339"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PKN51_08410"
     CDS             <1..855
                     /locus_tag="PKN51_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HNT85161.1"
                     /translation="LLSRATVYVLQSLTADDLMHIVARAQAQQALPAIDSVALERLLG
                     YADGDARRLLNTLETVAVTATAEGVAQIDEAWLLKVLGQQLRRYDKGGEQFYDTISAL
                     HKSVRGSDPDAALYWLVRMLDGGADPRYLARRMLRMASEDIGLADPRALRLALDAAEV
                     YERLGSPEGELALAECVVYLAVAPKSNAVYKAYNAARAWVRQDVTRPVPLHLRNAPTR
                     LMKQLDYGKGYRYAHDEEGGFAAGERYLPEGMPDQRFYDPVPRGLEIRIGEKLAELRR
                     RNREAAGG"
     gene            1092..2021
                     /locus_tag="PKN51_08415"
     CDS             1092..2021
                     /locus_tag="PKN51_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT85162.1"
                     /translation="MDILLQQIINGLVLGSMYALIALGYTMVYGIIQLINFAHGEVLM
                     VGALVAWTVIGVLQEAVPGMPTWLILLAATVAGCVVAAVLNIVIEKVAYRPLRNAPRL
                     APLITAIGMSILLQALAMVIWKPNVKSFPSMLPTEPYFIGEAVITPTQIMILVVTVMS
                     LAVLMYIVHYTRIGRAMRATAENPRVAALMGVKPDAVISATFAIGAVLAAIAGVMYAS
                     NYGTAQHFMGFLPGLKAFTAAVFGGIGNLTGAMVGGVLLGLIEAIGSGYLGALTGGLL
                     GSHYADIFAFIVLIIVLTLRPSGLLGERVADRA"
     gene            2041..3228
                     /locus_tag="PKN51_08420"
     CDS             2041..3228
                     /locus_tag="PKN51_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85163.1"
                     /translation="MTQKNKLIAFVLCGIALLVLPLILQQFGNAWVRIVNIALLYTML
                     ALGLNIVVGYAGLLDLGYVAFFAVGAYLFALLASPHLTDNFAAIAAMFPGGLHLSVWL
                     ALPLALLLAAIAGVLLGIPVLKLRGDYLAIVTLGFGEIIRIFLNNLDHPFNITNGPKG
                     VNQIDPVRFLGINLGKPLNIGGFEVASVSLYYYLFLVLVLVTVFVCQRLQDSRVGRAW
                     MAIREDEIAAKAMGLNVRNLKLLAFGMGASFGGVSGAMFAAFQGFVSPESFSLMESVM
                     IVAMVVLGGLGHLPGVVLGALLLSALPEVLRYVAGPLQQMTDGRLDAAILRQLFIALA
                     MIIIMLLRPRGLWPSPEHGKVKTEPADRITPLSGQQNPPGVDFPGDAVPGNTPHRQYP
                     VNP"
     gene            3251..4024
                     /locus_tag="PKN51_08425"
     CDS             3251..4024
                     /locus_tag="PKN51_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85164.1"
                     /translation="MSTDTVLKVQGVSKRFGGLQALSDVGISIQRGQVYGLIGPNGAG
                     KTTFFNVLTGLYTPDSGSFELAGQAYKPTAVHEVAKAGIARTFQNIRLFAEMTALENV
                     MVGRHVRTHSGLFGAVFRTAGFKAEEAAIAARAQELLDYVGIGKYAQFRARTLSYGDQ
                     RRLEIARALATDPQLVALDEPAAGMNATEKVQLRALIDRIRKDGRTILLIEHDVKLVM
                     GLCDRVTVLDYGKQIAEGTPAEVQKNEKVIEAYLGTGGH"
     gene            4026..4742
                     /locus_tag="PKN51_08430"
     CDS             4026..4742
                     /locus_tag="PKN51_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85165.1"
                     /translation="MAQTLLKVSGLKVAYGGIQAVKGVDFEVREGELVSLIGSNGAGK
                     TTTMKAITGSLPLAGGDIEYLGKSIKGQGPWDLVRQGLAMVPEGRGVFTRMSITENLL
                     MGAYIRSDKAGVAQDIERVFSIFPRLRERKDQLAGTMSGGEQQMLAMGRALMCRPKVL
                     LLDEPSMGLSPIMVDKIFEVVQDVYAQGVTVLLVEQNASRALALANRGYVMESGLVSM
                     SGSGKDLLADPKVRAAYLGE"
     gene            complement(4829..5809)
                     /locus_tag="PKN51_08435"
     CDS             complement(4829..5809)
                     /locus_tag="PKN51_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT85166.1"
                     /translation="MNRRSLLPVLSVALLASAIGAAPAAWAQGKPIRLIVPYAPGGPL
                     DVTARALAERVQPALGNVVVENKPGAGGNIGSDLVAKAAPDGSTIGIAAVATHAINPW
                     LFSKMPYDAGKDFAPITQMVRVPNVLVINADTAQRLGIRTLADLIAYARKNPGKLNYG
                     SGGNGSAGHLAGEMFKQGAGIFAVHIPYNGANPAQLALLAGQVDYNIDNLAAAAANIK
                     AGKIKALAVTSARPSPLLPGVPAMAETIKGFEVDTWWGLVAPAGTPREVIDRLNAAFT
                     DALKAPETQQRFAQLMAEPVPTTPEQFGQLMTRERARYQPVVKRSGAKVD"
     gene            5960..6286
                     /locus_tag="PKN51_08440"
     CDS             5960..6286
                     /locus_tag="PKN51_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85167.1"
                     /translation="MINRWLSVAVTTACMGVGVAHAQGVATPGVDQRQINQERRIEQG
                     VASGQLTPREAARMQRQQNRVAAAEADAKADGRVTATERARLHAMQDRNSRRIHRQKH
                     DRQRVR"
     gene            complement(6357..7400)
                     /locus_tag="PKN51_08445"
     CDS             complement(6357..7400)
                     /locus_tag="PKN51_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNT85168.1"
                     /translation="MTHTITVEPSGRSFQAEAGEPMLTAGIRQGIGLPYGCKDGACGS
                     CKVKKLSGQVTLGPHQDKALSAQEEADGYVLTCCGVATSDVVLESRQVTHAGAFPIKK
                     MPARVALLERLSHDVMRVRLQLPANDIIQYHAGQYVEFILRDGSRRSYSMANAPHTLI
                     ESGSPMVDLHIRHMPGGKFTDHVFGAMKEKEILRIEGPYGSFHLRDESPKPIVLLASG
                     TGFAPIKAIIEHMQFMGITREAVLYWGGRRPQDLYLDAWVRAKTQEMPHLRYVPVVSD
                     ALPEDGWGGRTGFVHRAVLQDLADLSGHQVYACGAPIVVDSARRDYVEQAGLPAEEFY
                     ADAFTSEADKHAG"
     gene            7450..8370
                     /locus_tag="PKN51_08450"
     CDS             7450..8370
                     /locus_tag="PKN51_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT85169.1"
                     /translation="MPSNQNPYGALPARFRRPRVLIVGCGDVGSRAARALPPRVRVLA
                     LARSAEAAAALRQQAIVPLAGDLDQAASLDRLAGIATHVLHLAPPPAVGEGDPRTAAL
                     LRALARRRAPLALVYGSTSGVYGDCGGAWVDETRPVRPQTARARRRVAAETWVRAFGR
                     AAGVRASILRIPGIYAPDRPGGTPRERLQRGTPVLAPADDVFTNHIHADDLARAFVLA
                     LWRGRPQRVVHACDDSQLKMGDYVDLAADLYGLPRPPRIDRQAADAQLPATLLSFMSE
                     SRRMHNTRLKRELRLRLRHPDVHTGLLPKT"
     gene            8483..9748
                     /locus_tag="PKN51_08455"
     CDS             8483..9748
                     /locus_tag="PKN51_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT85170.1"
                     /translation="MDFAYSTRVQALRQQVQRFMDDLILPSHRDWLRWSEAGAYPLDV
                     IEPLKARARAAGLWNLFLPGLRDDEPGTRLSNLEYAPLAEIMGRVPWASEVFNCSAPD
                     TGNIELLQLFATPAQRAHWLRPLMEGTMRSCFAMSEPDVASSDPTNLGTRIARDGDHW
                     VLNGRKWFITGAAHPQCRLAIVMGVSDAAPDVPAHRRHSLLLVPMDAPGLEIVRNIPV
                     MQHQAIEGHCELVFRDVRVPADHLLGTAGEGFAMAQARLGPGRVHHCMRTIGQCELAL
                     ELMAERALERMAFGRHLSDFANVQDWIACSRLEIDQARLLVLRAAWRMDTEGNAAART
                     DVSAIKVVAAQLQTRVLDRAMQVFGAVGLTPDTPLAALWSWGRALRFLDGPDEVHLRT
                     VAREELRRSRAHRGDNVLHLQRWMPPESL"
     gene            complement(9797..9883)
                     /locus_tag="PKN51_08460"
     tRNA            complement(9797..9883)
                     /locus_tag="PKN51_08460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9847..9849),aa:Leu,seq:caa)
     gene            9925..10827
                     /gene="cysM"
                     /locus_tag="PKN51_08465"
     CDS             9925..10827
                     /gene="cysM"
                     /locus_tag="PKN51_08465"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNT85171.1"
                     /translation="MDYPTIEDAIGRTPLVRLQRLGAQEHAARGSVVLAKLEGNNPAG
                     SVKDRPALSMIRRAEERGEIRPGDTLIEATSGNTGIALALAAAIRGYRMVLIMPEDLS
                     VERAQTMKAFGAELILTPRSGGMEYARDLADKMVAQGKGRVLDQFANPDNPRIHYETT
                     GPELWQQTGGRITHFVSAMGTTGTITGVSRFLKEQNPAVRIIGAQPAEGSRIPGIRKW
                     PEEYLPRIYDPAAVDELTLVSQADAEDMARRLAREEGIFGGISSGGAAWVAQQVAARE
                     QGATVVFVVCDRGDRYLSTGVFPA"
     gene            10898..11446
                     /locus_tag="PKN51_08470"
     CDS             10898..11446
                     /locus_tag="PKN51_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNT85172.1"
                     /translation="MSPFRYCPCCATPLAMIVQEEDSGPRERLRCPACDYTHWNNPVP
                     VLAAIIEYRGQVLLARNAAWPSKMFALITGFMEAGETPQDGIVREIKEETQLDTRSLA
                     LVGVYDFQRMNQVIIAFHAVCEGEVRLSPELADWRLYDPPQLKCWPAGTGYALADWLR
                     ARGHEPVFFTPEENAERRRGLD"
     gene            11461..11688
                     /locus_tag="PKN51_08475"
     CDS             11461..11688
                     /locus_tag="PKN51_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNT85173.1"
                     /translation="MDIAKEVDARGLNCPLPILKAKKALAELQSGQLLRVLSTDGGAL
                     RDFQAFARQTGNELVEQSTEGEVTAHVLRRR"
     gene            complement(11813..12700)
                     /gene="galU"
                     /locus_tag="PKN51_08480"
     CDS             complement(11813..12700)
                     /gene="galU"
                     /locus_tag="PKN51_08480"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNT85174.1"
                     /translation="MTASRQVLTAVFPVAGLGTRFLPATKASPKEMLPIVDKPLIQYA
                     VEEAYAAGVRHMVFVTGRSKRAIEDHFDTAYELESELEAAGKADLLAMVRAVQPDDMD
                     CTYVRQPRSLGLGHAVLCAAHIVGEQPFAVLLADDLMVGPADGQPVLAQMADAFESLG
                     RSVLAVQQVPADQVRRYGIVAGTPAGERLISIERIVEKPAPEQAPSRMGVAGRYILTP
                     RIFDEIRQQPPGAGGEIQLTDAIARLMQKERVYAYQYSGKRYDCGSKEGFLEATVELA
                     LQHPQMGAPFRDYLKTLSL"
     gene            12807..13772
                     /locus_tag="PKN51_08485"
     CDS             12807..13772
                     /locus_tag="PKN51_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT85175.1"
                     /translation="MDILLLEELVADARQWLEARHHVDYRPELLEDEALLRGRLYKVD
                     ALVVPPRLKINAQLLDYAPRLAAVGRIHDGTENLDFEACQKRHVRVIQASSATVRASA
                     EHQLCTLMQLFRSGGQVGPSLSLRRGAPGREINDSVIGLFGLAPPAHVLATMLVPLGA
                     RVVGYDPAVHRNAELWRRLGVQPMALADMLEVADAVSMQVIYASRYRGLVNDRVLAAC
                     KPGQLWTSISRPSLFDLDALAGALRSGRIGALMMDSDDERLTAPDSPLRHAPNVRITP
                     RLAPHTQESHLRGSWYLVDRIHEALMLNETRNQRGTLDSEPVPLD"
     gene            complement(13958..16846)
                     /locus_tag="PKN51_08490"
     CDS             complement(13958..16846)
                     /locus_tag="PKN51_08490"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNT85176.1"
                     /translation="MSESLSQPGLASLSKSFEPAAIEARWGPAWEQAGLGRAGYRGSG
                     QPDAGAAARGENFAIQLPPPNVTGTLHMGHAFNQTIMDSLTRYHRMRGFNTAWVPGTD
                     HAGIATQIVVERQLQEQGQSRHDLGRKNFVARVWDWKQQSGDTITRQMRRMGASVDWS
                     REYFTMDDRLSKVVTETFVQLYEQGLIYRGKRLVNWDPELKTAVSDLEVESEEEDGFL
                     WHIAYPLADGSGERLVVATTRPETMLGDVAVMVHPEDERYRRFIGKSVTLPLVGRDIP
                     VIADAYVDKDFGTGVVKVTPAHDANDYAVGQRHGLPMIGVLNLDATINDNAPAIFRGL
                     NRFAARKAIVADLEAQGLLVETRKHKLMVPRCARTGQIIEPMLTDQWFVAMNKAPTLP
                     AAHGARPGAGVATPPDARSIAQKAIDAVQSGQVRFVPEQWVNTYNQWMNNIQDWCISR
                     QLWWGHQIPAWYDEEGNVYVARNEAEAQAQAPGKTLRRDEDVLDTWYSSALVPFSTLG
                     WPEPTEDLNLYLPSSVLVTGHDIIFFWVARMIMMTTHFTGRVPFRDVYIHGLVLDAHG
                     KKMSKSEGNVLDPVDLIDGIALEPLLAKRTTGLRKPESAPRVRENTKKEFPDGIPGYG
                     ADALRFTFAAMATLGRTVNFDSKRCEGYRNFCNKLWNASRFVLMNCEGADCGVEGDTA
                     IERSAADRWIVSLLQQTEAAVAQGFADYRLDQVAQTIYDFVWNEYCDWYLEIAKVQIQ
                     TGKAAQQRATRRTLIRVLEAILRLAHPVIPFVTEALWQVVAPIAGKAGDSIATQPYPQ
                     AQPVHIDEAAQRQIGHLKALVDACRNLRGEMGVSPAQRLPLYALGEADFIRDQAPVLQ
                     ALAKLSEVKVFEHEADWAAAAQAAPVAVVGQARLALFMAVDVAAEKARLGKEAGRLEG
                     EIAKAQGKLNNPAFAAKAPPAVIEQENRRVAEFGAALAKIRDQLQRLG"
BASE COUNT         2586 a         5768 c         5975 g         2764 t
ORIGIN      
        1 ctgctgtcgc gcgccacggt gtacgtgctg caatcgctga cggccgacga tttgatgcat
       61 attgtggccc gggcgcaggc gcagcaagcg ctgccagcta tcgattcggt agctttggag
      121 cgcctgctcg gctatgccga cggcgacgcc cggcgcctgc tcaacaccct ggagacggtg
      181 gccgtgaccg ccaccgcgga gggtgtggcg cagatcgacg aggcctggct gctgaaggtg
      241 ctgggccagc agctgcgccg ctacgacaag ggcggcgagc agttctacga caccatcagc
      301 gcgctgcaca agtcggtgcg tggctcggat cccgacgccg ccctgtactg gctggtgcgc
      361 atgctcgacg gcggggccga cccgcgctac ctggcgcgcc gcatgctgcg catggccagc
      421 gaagacatcg gcctggccga cccgcgcgcg ctgcgcctgg cgctggatgc ggccgaggtg
      481 tacgagcgcc tgggcagccc cgagggcgag ctggcgctgg ccgagtgcgt ggtctacctg
      541 gcggtggctc ccaagagcaa cgcggtatac aaggcttaca acgccgcccg cgcctgggtg
      601 cggcaggacg tgacacggcc ggtgccgttg catctgcgca acgcgccgac ccggctgatg
      661 aaacagctcg actacggcaa gggttaccgc tacgcgcacg acgaagaggg cggctttgcc
      721 gccggcgagc gctacctgcc cgagggcatg cccgatcagc gcttttacga cccggtgccg
      781 cgcgggctgg agatccgcat cggcgaaaag ctggccgagc tgcgccgacg caaccgcgag
      841 gccgctggcg gctgaggcgt acgcgctggc tggggcgcgc gcggccgccg tctacaaggg
      901 aaatcccggg tcgggcagat cgtccgcaac acccggcgtg ccgataaaat cgcgctcgct
      961 ttttgaaccc gcaacggagt ctccggcggg ttttttgtta gccgaccggc aagcccacaa
     1021 ggcgacgcca ggcggccgac tgaaccacgc cgcgccggag acaacggtgc ggacccttga
     1081 ggaagtctcg catggacatt ctgcttcagc agatcatcaa cggcctggtg ctgggcagca
     1141 tgtacgccct catcgccctg ggctacacca tggtttacgg catcatccag ctcatcaact
     1201 tcgctcacgg cgaggtgttg atggtgggcg cactggtggc ctggaccgtc atcggcgtgc
     1261 tgcaggaggc ggtgcccggc atgcccacct ggctgatctt gctggcggcc accgtggccg
     1321 gctgtgtggt ggcggcggtg ctcaacatcg tgatcgagaa ggtcgcttac cggccgctgc
     1381 gcaacgcccc acgcctggcg ccgctgatca ccgccatcgg catgtcgatc ctgctgcagg
     1441 cgctggccat ggtgatctgg aaacccaacg tcaagtcctt cccgtccatg ctgccgaccg
     1501 agccgtactt catcggcgag gcggtgatca cgccgacgca gatcatgatc ctggtggtca
     1561 cggtcatgtc gctggcggtg ctgatgtaca tcgtgcacta cacgcgcatc ggccgcgcca
     1621 tgcgcgccac cgccgaaaac ccgcgcgtgg cggcgctgat gggtgtcaag cccgacgcgg
     1681 tgatttcggc caccttcgcc atcggtgcgg tgctggcggc cattgccggg gtgatgtacg
     1741 cctccaacta cggcacggca cagcacttca tgggcttcct gccggggctg aaggccttca
     1801 cggcggcggt gttcggcggc atcggcaacc tgaccggcgc catggtgggc ggcgtgctgc
     1861 tggggctgat cgaggccatc ggctcgggct acctgggcgc gctcaccggc ggcctgctgg
     1921 gcagccacta cgccgacatc tttgccttca tcgtgctgat catcgtgctg acgctgcggc
     1981 cctcgggcct gctgggcgag cgcgtggcgg accgcgcctg acgcccggga ggaccttgcc
     2041 atgacgcaga agaacaagct gatcgccttc gtgctgtgcg gcatcgcgct gctggtgctg
     2101 ccgctgatcc tgcagcagtt cggcaacgcc tgggtgcgca tcgtcaacat cgcgctgctc
     2161 tacaccatgc tggcgctggg gctgaacatc gtggtcggct acgccggcct gctggacctg
     2221 ggctacgtcg ccttcttcgc ggtgggggcg tacctgttcg ccttgctggc ctcgccgcac
     2281 ctgaccgaca acttcgccgc catcgccgcc atgttcccgg gcggattgca tctgtcggtg
     2341 tggctggcgc tgccactggc gctgctgctg gcggccatcg cgggggtgct gctgggcatt
     2401 ccggtgctca agctgcgcgg cgactacctg gccatcgtca cgctgggctt cggcgagatc
     2461 atccgcatct tcctgaacaa cctcgaccac cccttcaaca tcaccaacgg ccccaagggc
     2521 gtcaaccaga tcgatccggt gcggtttctc ggcatcaacc tgggcaagcc gctgaacatc
     2581 ggcggcttcg aagtcgcctc ggtgtcgctg tactactacc tgttcctggt gctggtgctg
     2641 gtcacggtgt tcgtgtgcca gcgcctgcag gattcgcgcg tcggccgcgc ctggatggcg
     2701 atccgcgaag acgagatcgc cgccaaggcc atgggcctga acgtgcgcaa cctcaagctg
     2761 ctggccttcg gcatgggggc gtcgttcggc ggcgtgtcgg gagccatgtt cgccgctttc
     2821 cagggctttg tctcgcccga gtcgttcagc ctgatggagt cggtgatgat cgtcgccatg
     2881 gtggtgctgg gcggtctggg ccacctgccc ggcgtggtgc tgggggcgct gctgctgtcg
     2941 gcgctgccgg aggtgctgcg ctatgtggcc gggccgctgc agcagatgac cgacggccgg
     3001 ctggacgcgg ccatcctgcg ccagttgttc atcgcgctgg ccatgatcat catcatgctg
     3061 ctgcgtccgc gcggcctgtg gccgtcgccc gagcacggca aggtcaagac cgagccggcc
     3121 gaccgcatca ccccgctgtc ggggcagcag aacccgccgg gggtggactt ccccggtgac
     3181 gccgtgcccg gcaacacgcc gcaccggcag taccccgtca atccctgaaa cccaaccgga
     3241 gacgaacgtc atgagcacag acaccgtcct gaaggttcag ggtgtttcca agcgcttcgg
     3301 cggcctgcag gccctgtccg acgtcggcat cagcattcag cgtggccagg tgtatggcct
     3361 gatcggcccc aacggcgccg gcaagaccac cttcttcaat gtgctgaccg ggctgtacac
     3421 gcccgacagc ggcagcttcg agctggccgg ccaggcctac aagccgaccg ctgtgcatga
     3481 agtggccaag gccggcatcg cgcgcacctt ccagaacatc cgcctgttcg ccgagatgac
     3541 ggcgctggaa aacgtgatgg tcgggcgcca cgtgcgcacc cattccggcc tgttcggcgc
     3601 cgtcttccgc accgccggct tcaaggccga ggaggcggcc atcgccgccc gtgcgcagga
     3661 gctgctggac tacgtgggca tcggcaagta cgcccagttc cgcgcccgca cgctgtctta
     3721 tggcgatcag cgccggctgg aaatcgcccg cgcgctggcc accgacccgc aactggtggc
     3781 gctggacgag ccggccgcgg gcatgaacgc caccgagaag gtgcagttgc gcgcgctgat
     3841 cgaccgcatc cgcaaggacg gccgcaccat tctgctgatc gagcacgacg tgaagctggt
     3901 gatgggcctg tgcgaccgcg tcacggtgct cgactacggc aagcagatcg ccgagggcac
     3961 gccggccgag gtgcagaaga acgagaaagt catcgaggcc tatctgggca cgggaggcca
     4021 ctgacatggc acagacactg ttgaaagtca gcggcctgaa ggtggcctat ggtggcatcc
     4081 aggccgtcaa gggcgtggac ttcgaggtgc gcgaaggcga gttggtgtcg ctgatcggct
     4141 ccaacggcgc cggcaagacc accaccatga aggccatcac cggctcgctg ccgctggccg
     4201 gtggcgacat cgagtacctg ggcaagtcca tcaagggcca gggaccgtgg gatctggtgc
     4261 gccagggcct ggccatggtg cccgaaggcc gcggcgtgtt cacgcgcatg agcatcaccg
     4321 aaaacctgct gatgggtgcc tacatccgga gcgacaaggc cggtgtggcg caggacatcg
     4381 agcgcgtgtt cagcatcttc ccgcgcctgc gtgagcgcaa ggaccagctg gccggcacca
     4441 tgtcgggcgg cgaacagcag atgctggcca tgggccgggc gctgatgtgc cggcccaagg
     4501 tgctgctgct ggacgagccg tccatgggct tgtcgcccat catggtggac aagattttcg
     4561 aggtggtgca ggacgtctac gcccagggcg tcaccgtgct gctggtggag cagaacgcca
     4621 gccgcgcgct ggcgctggcc aaccgcggct acgtgatgga gtcgggcctg gtcagcatga
     4681 gcggcagcgg caaggacctg ctggccgacc cgaaagtgcg cgcggcctac ctgggcgaat
     4741 aacgccgctg ccgtgcccca acgccaaagc cacgccctgg cggcgtggct tttttgtggt
     4801 tcggggcggg ctgctgccgt ttctagggtc agtccacctt ggcacccgag cgcttgacca
     4861 ccggctggta gcgggcgcgc tcgcgcgtca tcaactggcc gaattgctcg ggcgtggtcg
     4921 gcaccggctc ggccatcagc tgggcaaagc gctgctgcgt ttcgggcgcc ttgagtgcgt
     4981 cggtgaaggc ggcgttgagc cggtcgatca cttcgcgcgg cgtgcccgcg ggcgccacca
     5041 gaccccacca ggtatcgacc tcgaagccct tgatggtctc ggccatggcg ggcacgcccg
     5101 gcagcagggg cgaggggcgg gcgctggtga cggccagcgc cttgatcttg ccggccttga
     5161 tgttggccgc cgccgccgcc aggttgtcga tgttgtagtc gacctggccg gccagcagtg
     5221 ccagctgcgc cgggttggcg ccgttgtacg ggatgtgcac ggcaaaaatg cccgcgccct
     5281 gcttgaacat ctcgccggcc aggtggcccg ccgagccgtt gccgcccgag ccgtagttca
     5341 gcttgccggg gttcttgcgg gcgtaggcga tcaggtcggc cagggtgcgg atgcccaggc
     5401 gctgcgcggt gtcggcgttg atgaccagca cgttgggcac acgcaccatc tgggtgatgg
     5461 gggcgaagtc cttgccggcg tcgtagggca tcttgctgaa cagccagggg ttgatggcgt
     5521 gcgtggccac cgccgcgatg ccgatggtgc tgccgtcggg cgcggccttg gccaccaggt
     5581 cggagccgat gttgccgccg gcgccgggtt tgttttcgac caccacgttg ccgagcgcgg
     5641 gctgcacccg ctcggccagt gcgcgggccg tgacgtcgag cgggccgccg ggggcgtagg
     5701 gcacgatcag ccggatcggc ttgccttgcg cccaggccgc gggcgcggcg ccgatggcgc
     5761 tggcaagcag agccaccgac agaacaggaa ggagcgagcg gcgattcatg gtcatgagcg
     5821 tgtgaaaaga agtgaacaac ggacgcacga actttacgcg agctttgcct gcgcctcgtc
     5881 ggcggggcgc cgcgccctgc gttgtgcagt gtgggcgggt cgccgacccg ttccatcaga
     5941 cacttgaagg agaaacatca tgatcaatcg atggctgagc gtggccgtga ccacggcgtg
     6001 catgggtgtg ggcgtggccc atgcgcaagg ggtcgccacc ccgggcgtgg atcagcgtca
     6061 gatcaatcag gagcgtcgca tcgagcaggg cgtggcctcg ggtcagctga cgccgcgcga
     6121 ggcggcgcgc atgcagcgcc agcagaaccg ggtggcggcg gccgaagctg acgccaaggc
     6181 ggacgggcgg gtgaccgcca ccgagcgcgc ccgtctgcac gccatgcagg atcgcaacag
     6241 ccgtcgtatc catcgccaga agcacgatcg ccagcgcgtg cgctgacctg tcgtgcccga
     6301 aggtgtggac gaacgccggg ccaccgcagg gaaagccccg gcgacacaga ctgccgtcag
     6361 ccggcgtgct tgtcggcttc gctggtgaag gcgtcggcgt agaactcttc ggccggcagg
     6421 ccggcctgct cgacatagtc ccggcgcgcc gaatcgacca cgatcggcgc gccgcaggcg
     6481 tacacctgat ggcccgacag gtcggccaga tcctgcagca ccgcgcggtg cacgaagccg
     6541 gtgcgcccgc cccagccgtc ttcgggcagc gcgtccgaca ccaccggcac gtagcgcagg
     6601 tgcggcatct cctgcgtctt ggcgcgcacc caggcgtcca ggtacaggtc ctgcgggcgc
     6661 cggccacccc agtacagcac ggcctcgcgc gtgatgccca tgaactgcat gtgctcgatg
     6721 atggccttga tgggcgcaaa acccgtgccc gaggccagca gcacgatcgg cttgggcgat
     6781 tcgtcgcgca ggtgaaagct gccgtagggc ccttcgatgc gcaggatttc cttttccttc
     6841 atggcgccga acacatggtc ggtgaacttg ccgcccggca tgtggcggat gtgcaggtcg
     6901 accatgggcg agccgctttc gatcagcgtg tgcggcgcat tggccatgct gtagctgcgg
     6961 cggctgccgt cgcgcaggat gaattcgacg tactggcccg cgtggtactg gatgatgtcg
     7021 ttggccggca gttgcagccg cacccgcatc acgtcgtgcg acaggcgctc cagcagcgcc
     7081 acgcgcgccg gcatcttctt gatcggaaag gcgccggcgt gcgtgacctg gcgcgactcc
     7141 agcaccacgt cgctggtggc cacgccgcag caggtcagca cgtagccgtc ggcttcttcc
     7201 tgggccgaca gggccttgtc ctggtgcggg ccgagcgtca cctggccgga gagcttcttc
     7261 accttgcacg agccgcaggc gccgtccttg cagccatagg gcaggccgat gccctggcgg
     7321 atgccggcgg tgagcatggg ttcgcccgct tcggcctgaa agctgcggcc gctcggttcg
     7381 acggtgatgg tgtgggtcat gccttggtta tcctgtggcg gtcttgcatg gcggaagaaa
     7441 gcgaagattt tgccctcaaa ccagaaccct tacggcgcgc tgccggcgcg ctttcggcgc
     7501 ccgcgcgtgc tgatcgtcgg ttgcggcgac gtcggctcgc gggcggcacg ggcgttgccg
     7561 ccacgcgtgc gcgtgctggc gctggcgcgc tcggccgagg cggcggcggc gctgcgccag
     7621 caggccatcg tgccgctggc cggcgatctg gaccaggccg ccagcctgga ccggctggcc
     7681 ggcatcgcca cccatgtgct ccatctggcg ccgccgcccg cggtgggtga gggcgacccg
     7741 cgcaccgccg cgctgctgcg cgcgctggcc cggcggcgcg cgccgctggc gctggtgtat
     7801 ggctccacca gcggcgtgta cggcgactgc ggcggcgcct gggtggacga gacgcgccca
     7861 gtgcggccgc agacggcgcg tgcgcggcgc cgcgtggcgg ccgaaacctg ggtgcgtgcc
     7921 tttggccgcg ccgccggcgt gcgcgccagc atcctgcgca ttcccggcat ctacgcgccc
     7981 gaccgccccg gcggcacgcc gcgcgagcgc ctgcagcggg gcacgccggt gctggcgccg
     8041 gccgacgacg tgttcaccaa ccacatccac gccgacgatc tggcgcgcgc ctttgtgctg
     8101 gcgctgtggc gcggccggcc gcagcgcgtg gtgcatgcct gcgacgacag ccagctgaag
     8161 atgggcgatt acgtcgatct ggcggccgac ctgtacggcc tgccgcggcc gccgcgcatc
     8221 gaccgccagg ccgcagacgc gcagctgccg gcaacgctgc tgtcgttcat gagcgaatcg
     8281 cgccgcatgc acaacacgcg cctcaagcgc gagctgcgcc tgcgcctgcg ccaccccgac
     8341 gtgcacaccg gattgctacc aaaaacatag ctgctggcgc tttctggaca agcgcgagag
     8401 cctgatttga tcataagact tggccaggcc cgtgtcgtgc acaggactgg cgccgtggtg
     8461 ctgcttgcgt acagtcaagt ccatggactt tgcctattcg acccgggtgc aggcgttgcg
     8521 tcagcaggtg cagcgtttca tggacgacct gatcctgcct tcgcaccgcg actggctgcg
     8581 ctggtccgag gccggtgcct acccgctcga cgtgatcgag ccgctgaagg cgcgcgcccg
     8641 cgccgccggt ctgtggaacc tgttcctgcc cggtctgcgc gacgacgaac ccggcacccg
     8701 cctgagcaac ctcgaatacg cgccgctggc cgaaatcatg ggtcgcgtgc cctgggccag
     8761 cgaggtgttc aactgcagcg cgccagacac cggcaacatc gagctgctgc agctgttcgc
     8821 caccccggcg cagcgcgcgc actggctgcg gccgctgatg gaaggcacga tgcgctcctg
     8881 ctttgccatg agcgagccgg acgtcgcctc atccgacccc accaacctgg gcacacgcat
     8941 tgcgcgcgat ggcgaccact gggtgctgaa cggccgcaag tggttcatca ccggcgccgc
     9001 gcacccccaa tgccgcctgg ccatcgtgat gggcgtgtcc gatgccgccc ccgatgtgcc
     9061 cgcgcaccgc cgacacagcc tgctgctggt gccgatggac gcgccgggtc tggagatcgt
     9121 gcgcaacatc ccggtgatgc agcaccaggc catcgaaggg cattgcgagc tggtgtttcg
     9181 tgacgtgcgc gtgccggccg atcacttgct gggcaccgcc ggcgagggct ttgccatggc
     9241 ccaggcgcgg ctggggccgg ggcgcgtgca ccactgcatg cgcaccatcg gccagtgcga
     9301 gctggcgctg gaactgatgg ccgagcgcgc cctggagcgc atggccttcg gcaggcattt
     9361 gtcggacttc gccaacgtgc aggactggat cgcctgcagc cggcttgaaa tcgaccaggc
     9421 gcgcctgctg gtgctgcgcg ccgcctggcg catggacacc gagggcaacg ccgccgcccg
     9481 caccgacgtc tcggccatca aggtggtggc agcgcagttg cagacgcgcg tgcttgatcg
     9541 cgccatgcag gtgttcggtg ccgtcggcct gacgcccgac acgccgctgg ccgcactgtg
     9601 gagctggggc cgggcgctgc gctttctgga cggccccgat gaagtgcatc tgcgcaccgt
     9661 ggcacgcgaa gagctgcgcc gcagccgcgc gcaccgcggc gacaacgtgc tgcacctgca
     9721 gcgctggatg ccgcccgaaa gcctgtgaaa gcaggcaagc cgcacgcccg atgcaggcgt
     9781 gcggcttgcg gcaatgtggt gcccggggcc ggaatcgaac cggcacggtg ttgccaccgg
     9841 cagattttga gtctgctgcg tctaccgatt tcgccacccg ggcagcgcga tagcacagcc
     9901 aaaaattatg gcacagtgca tggcatggac tacccgacga tcgaggatgc gattgggcgc
     9961 acgccgctgg tgcggctgca gcgcctgggc gcgcaggagc acgccgcgcg cggcagcgtg
    10021 gtgctggcca agctggaagg caacaacccg gccggctcgg tcaaggaccg accggcgctg
    10081 tcgatgatcc gccgcgccga agaacgtggc gagatccgcc ccggcgacac cctgatcgaa
    10141 gccaccagcg gcaacaccgg catcgcgctg gccttggcag cggcgatccg cggctaccgc
    10201 atggtgctga tcatgccgga ggacctgtcg gtcgagcgcg cccagaccat gaaggccttc
    10261 ggcgccgagc tgatcctcac cccgcgctcg ggcggcatgg agtacgcgcg cgacctggcc
    10321 gacaagatgg tggcgcaggg caagggccgc gtgctggatc agttcgccaa ccccgacaac
    10381 ccgcgcatcc actacgaaac caccggcccc gagctgtggc agcagaccgg cgggcgcatc
    10441 acgcacttcg tctcggccat gggcaccacc ggcaccatca ccggcgtgtc gcgctttctg
    10501 aaggaacaga acccggccgt gcgcatcatc ggcgcgcagc cggccgaggg ctcgcgcatc
    10561 ccgggcatcc gcaagtggcc cgaggaatac ctgccacgca tctacgaccc cgccgcggtg
    10621 gacgagctga ccctggtcag ccaggccgac gccgaggaca tggcgcgccg gctggcgcgc
    10681 gaggagggca tattcggcgg catctcctcc ggcggcgcgg cctgggtggc gcagcaggtg
    10741 gcggcgcgcg agcagggggc gaccgtggtc ttcgtggtgt gcgaccgggg cgaccgctac
    10801 ctgtccaccg gcgtgttccc ggcctgaggc tttcacaatc caaccggccg ctggcgcttt
    10861 actgacaagc gcaagcagct atgaataata tagtaacatg agcccgttcc gctattgccc
    10921 ctgctgcgcc acgccgctgg ccatgatcgt gcaggaagaa gacagcggcc ccagggagcg
    10981 cctgcgctgc ccggcctgcg actacacgca ctggaacaac ccggtgccgg tgctggcggc
    11041 catcatcgaa taccgcggcc aggtgctgct ggcgcgcaac gccgcctggc cgagcaagat
    11101 gttcgcgctc atcaccggct tcatggaggc gggcgagacg ccgcaggacg gcatcgtgcg
    11161 cgaaatcaag gaagaaacgc agctcgacac gcgctcgctg gcgctggtgg gcgtgtacga
    11221 cttccagcgc atgaaccagg tcatcatcgc ctttcatgcc gtctgcgagg gcgaggtgcg
    11281 gctgtcgccc gaactggccg actggcgcct gtacgacccg ccccagctca agtgctggcc
    11341 ggccggcacc ggctacgccc tggccgactg gctgcgcgcg cgcgggcatg agccggtatt
    11401 tttcacgccc gaagaaaacg ccgagcgacg ccgcggtctg gattgaccga caatcgtccg
    11461 atggatatcg ccaaggaagt cgacgcgcgc gggctgaatt gcccgctgcc catcctcaag
    11521 gccaaaaagg cgctggccga gctgcaaagc gggcaactgc tgcgcgtgct gtcgaccgac
    11581 ggcggcgcgc tgcgcgactt tcaggccttt gcccgccaga ccggcaacga actggtcgag
    11641 cagagcaccg agggcgaggt gaccgcccac gtactgcggc ggcgctgatc cgtcggccca
    11701 gcgacggccg cgcaacgcgt ccgccaggcg ctggccgacg aagccacgcc gccccgcagg
    11761 ccctgcggcg cacgccaaga gcgcaagcgg gccgccaccg tcgcgtcccg cgtcagaggc
    11821 tgagcgtctt caggtagtcg cgaaacggcg cgcccatctg cgggtgctgc agggccagct
    11881 ccacggtggc ttccagaaag ccttctttgc taccacaatc gtagcgtttg ccgctgtact
    11941 ggtaagcgta gacgcgctct ttctgcatca ggcgggcgat ggcgtcggtg agctggatct
    12001 cgccgccggc cccgggcggc tgctggcgga tctcgtcgaa gatgcgcggc gtcaggatgt
    12061 agcggcccgc cacccccatg cgcgaaggcg cctgctcggg ggccggcttt tcgacgatgc
    12121 gttcgatgct gatcagccgc tcgccggccg gcgtgccggc cacgatgccg tagcggcgca
    12181 cctgatcggc cggcacctgc tgcaccgcca gcaccgaacg gccgagcgac tcgaaggcgt
    12241 cggccatctg cgccagcacc ggctggccgt cggccgggcc gaccatcagg tcgtcggcca
    12301 gcagcaccgc gaacggctgc tcgcccacga tgtgcgccgc gcacagcacg gcatggccca
    12361 ggccgagcga gcgcggctgg cgcacatagg tgcagtccat gtcgtcgggc tgcacggcgc
    12421 gcaccatggc cagcagatcg gccttgccgg cggcctccag ctcgctttcc agttcgtagg
    12481 cggtgtcgaa gtggtcctcg atggcgcgct tgctgcggcc ggtgacgaac accatgtggc
    12541 gcacgccggc ggcgtaggct tcttcgacgg cgtactggat cagcggtttg tcgacgatgg
    12601 gcaacatttc cttcgggctg gccttggtgg ccggcaggaa gcgcgtgccc aggccggcga
    12661 cggggaacac ggcggtgaga acctggcgcg atgcggtcat gatcggttgg cggggtggta
    12721 aagtgacaag ctgactgtct gcccctctga tacggccagc acacgccggc aaggcaggtc
    12781 aaaacgacaa ctcgaagagg gaacgcttgg atattctcct gctggaggaa ctggtggccg
    12841 acgcacgcca gtggctggaa gcgcggcacc acgtggatta tcggccagag ctgctggagg
    12901 acgaggcctt gctgcgcggt aggctgtaca aggtcgacgc gctggtggtg ccgccgcgcc
    12961 tcaagatcaa cgcccagctg ctggactacg cgccgcgcct ggcggccgtg ggccgcatcc
    13021 acgacggtac cgagaacctg gatttcgaag cctgccagaa gcgccatgtg cgtgtgatcc
    13081 aggcctccag cgccaccgtg cgcgccagcg ccgagcacca gctgtgcacc ttgatgcagc
    13141 tgtttcgctc cggcgggcag gttggaccct cgctgtcgct gcgtcgtggc gcgccggggc
    13201 gcgagatcaa cgacagcgtg atcggcctgt tcggcctggc gccgccggcg cacgtgctgg
    13261 ccaccatgct ggtgccgttg ggtgcgcgcg tggtgggcta cgacccggcg gtgcaccgca
    13321 acgccgaact gtggcgccgg ctgggcgtgc agccgatggc gctggccgac atgctggagg
    13381 tggccgacgc ggtgtcgatg caggtcatct atgcctcacg ctaccggggt ctggtcaacg
    13441 atcgtgtgct ggcagcctgc aagccggggc agctgtggac cagcatcagc cgcccgtcgc
    13501 tgttcgatct cgacgccctg gccggcgcgc tgcgctcggg gcgcatcggc gcgctcatga
    13561 tggacagcga cgacgagcgc ctgacggccc ccgattcgcc gctgcgtcac gcgcccaacg
    13621 tgcgcatcac gccccggctg gcgccccaca cgcaggaatc gcacctgcgc ggcagttggt
    13681 acctggtcga ccgcatccat gaggcgctga tgctgaacga aacccgcaac cagcgcggca
    13741 cactggacag cgagccggtt ccgctggact gagaaggtgt gggcgggcag cccatgcccg
    13801 cctggaccgc ccccaaggca cgctggttgt tgcccatgct ggccgtggcc cgggtcacgg
    13861 cggtgcctgg cgcctgaaac cgccacgcgg cggcgctccc cctcgggcgc ccggaacccc
    13921 ttcttgcctg ccgaccggcc ggcgcgacgg gccgctttca gcccaggcgc tgcaactgat
    13981 cgcggatctt ggccagcgcc gcaccgaatt cggccacgcg ccggttttcc tgctcgatca
    14041 cggccggcgg cgccttggcg gcgaacgccg ggttgttcag cttgccctgt gccttggcga
    14101 tttcgccttc cagccggccc gcttccttgc ccagacgggc cttctcggca gccacgtcaa
    14161 ccgccatgaa cagcgccagc cgcgcctgac ccaccaccgc caccggcgcc gcctgcgcgg
    14221 cggcggccca gtcggcttcg tgctcgaaca ccttcacctc gctcagcttg gccagcgcct
    14281 gcagcaccgg cgcctggtcc cggatgaagt cggcctcgcc cagcgcgtac agcggcaggc
    14341 gctgggcggg tgagacgccc atttcgccgc gcaggttgcg gcaggcgtcg accagcgcct
    14401 tcagatgccc gatctggcgc tgcgccgcct cgtcgatgtg gactggctgc gcctgcgggt
    14461 agggctgggt ggcgatgctg tcgcccgcct tgccggcgat cggcgccacc acctgccaca
    14521 gcgcctcggt gacgaagggg atgacggggt gggccagccg caggatggct tccagcacac
    14581 ggatcagcgt gcggcgcgtg gcgcgctgct gggccgcttt gccggtctgg atctgcacct
    14641 tggcgatttc caggtaccag tcgcagtact cgttccagac gaagtcatag atcgtctgcg
    14701 ccacctggtc gagccggtag tcggcaaagc cctgggccac ggcggcttcg gtctgctgca
    14761 gcaggctgac gatccagcgg tcggcggccg agcgctcgat cgcggtgtcg ccctcgacgc
    14821 cgcagtcggc accttcgcag ttcatcagca cgaagcggct ggcgttccag agcttgttgc
    14881 agaagttgcg gtagccctcg cagcgcttgc tgtcgaagtt gacggtgcgc cccagcgtgg
    14941 ccatggcggc aaaggtgaag cgcagcgcgt cggcgccata gcccggaatg ccgtccggga
    15001 actccttctt cgtgttttcg cgcaccctgg gcgcggactc gggcttgcgc aggccggtgg
    15061 tgcgcttggc cagcagtggc tccagcgcga tgccgtcgat cagatcgacc gggtcgagca
    15121 cgttgccctc gctcttgctc atcttcttgc cgtgcgcgtc cagcaccagg ccgtggatgt
    15181 agacgtcgcg gaacggcacg cggccggtga aatgtgtggt catcatgatc atccgggcga
    15241 cccagaagaa gatgatgtcg tggccggtga ccagcacgct gctgggcagg tacaggttga
    15301 ggtcctcggt cggttcgggc cagcccaggg tggagaaggg caccagggcc gacgagtacc
    15361 aggtgtccag cacgtcttca tcgcggcgca gcgtcttgcc gggtgcctgg gcctgcgcct
    15421 cggcctcgtt gcgggcgacg tagacgttgc cctcttcgtc gtaccaggcc gggatctgat
    15481 ggccccacca gagctggcgc gagatgcacc agtcctggat gttgttcatc cactggttgt
    15541 aggtgttgac ccactgctcg ggcacgaagc gcacctggcc ggactgcacg gcgtcgatgg
    15601 ccttctgcgc gatggatcga gcatcaggcg gcgttgcgac cccggcgccg ggccgcgcac
    15661 cgtgcgcagc agggagcgtg ggggccttgt tcatggccac gaaccactgg tccgtgagca
    15721 tcggctcgat gatctggccg gtgcgggcgc agcgcggcac catcagcttg tgctttctgg
    15781 tctcgaccag caggccctgg gcctcgaggt cggcgacgat ggccttgcgg gcggcaaagc
    15841 ggttcaggcc gcggaagatc gccggcgcgt tgtcgttgat cgtcgcgtcc aggttcagca
    15901 cgccgatcat cggcaggccg tggcgctggc ccaccgcgta gtcgttggcg tcgtgcgccg
    15961 gcgtgacctt gaccacgccg gtgccgaagt ccttgtccac gtaggcgtcg gcgatcaccg
    16021 gaatgtcacg gcccaccagc ggcagcgtga cgcttttgcc gatgaagcgc cggtagcgtt
    16081 cgtcctcggg gtgcaccatc accgccacgt cgcccagcat ggtctcgggc cgcgtggtgg
    16141 ccaccaccag gcgctcgccg ctgccgtcgg ccagcgggta ggcgatgtgc cacagaaagc
    16201 cgtcttcttc ctcgctttcg acctcaaggt cgctgacggc ggtcttgagt tcggggtccc
    16261 agttgaccag gcgcttgccg cggtagatca ggccctgctc gtacagttgc acgaaggttt
    16321 cggtcaccac cttggacagt ctgtcgtcca tggtgaagta ttcgcggctc cagtccacgc
    16381 tggcacccat gcggcgcatc tggcgggtga tggtgtcgcc cgactgctgc ttccagtccc
    16441 acacgcgggc gacgaagttc ttgcgcccca ggtcgtggcg gctctggccc tgctcctgca
    16501 actggcgctc caccacgatc tgggtggcga tgccggcgtg atcggtgccc ggtacccagg
    16561 cggtgttgaa gccgcgcatg cggtggtagc gcgtcaggct gtccatgatg gtctggttga
    16621 acgcatgccc catgtgcagc gtgccggtga cgttgggcgg cggcaactga atggcgaagt
    16681 tctcgccacg tgcggcggcg ccggcgtccg gctggccgct gccacggtag cccgcccgcc
    16741 ccaggcccgc ctgctcccac gcggggcccc agcgcgcctc gatggcggcg ggttcgaagg
    16801 acttggacag ggacgcgagg cccggttgcg acagcgattc agacatggca gaggcggcct
    16861 gggccgcgaa cggagaggaa cgcgccgcag cgcagccggc cgggcgcaaa aaccgtccat
    16921 tctagcggcg cgcgcagggg cacccaggct tgggcggcac ctcccaatga agtgtaaaaa
    16981 aggcctctgg cgcttgattg gcaagcgtta aaagctatca aaacgatagt ttttcggagc
    17041 gcttgggtgc tgcgatcagg cgcggcgggg cggcacgcct tgccacccgc aag
//
LOCUS       DAOQTZ010000061        16977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3854_length_16977_cov_11.121380, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000061 DAOQTZ010000000
VERSION     DAOQTZ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16977
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3854_length_16977_cov_11.121380"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PKN51_08495"
     CDS             <1..155
                     /locus_tag="PKN51_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNT85177.1"
                     /translation="EFAAREQGYTFVSHQQEVGAGYFDDVTTVIQGGSSSVKALTGST
                     EEEQFH"
     gene            complement(389..760)
                     /locus_tag="PKN51_08500"
     CDS             complement(389..760)
                     /locus_tag="PKN51_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85178.1"
                     /translation="MTLTSHTPSRRALLAGCLAGATALAGCMVAPVPGAVYTDRDGVV
                     TSTTVYAPVAPPAPYYEVQPALPYPGAVWIGGYWRWGGHRHVWVPGRYERPRPGHHYV
                     PHRWHRSPHGGWYLQGGAWMR"
     gene            954..2423
                     /gene="guaB"
                     /locus_tag="PKN51_08505"
     CDS             954..2423
                     /gene="guaB"
                     /locus_tag="PKN51_08505"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNT85179.1"
                     /translation="MRLIGQALTFDDVLLVPAYSQVLPKDVNLSTQFTRRIRLNLPLV
                     SAAMDTVTEARLAIAMAQEGGIGIVHKNLTAHEQATQVAKVKRYESGVLRDPVVITPE
                     HTVLQVMQLSDELDISGFPVIDHGRVVGIVTGRDLRFETRYDVPVREIMTPRNKLITV
                     PEGTTLDVAKALLNKHRLERLLVVDEQDRLKGLITVKDITKQTTFPNAARDAHGRLRV
                     GAAVGVGEGTEERVEALVKAGVDALVVDTAHGHSQGVIERVRWVKNKYPQVDVIGGNI
                     ATGKAARALVDAGADGVKVGIGPGSICTTRIVAGVGVPQITAIHDVSQALEGTGVPMI
                     ADGGVRYSGDIAKAIAAGAHSVMMGGMFAGTEEAPGEIVLFQGRSYKSYRGMGSIGAM
                     QQGSADRYFQESSTGNPNADKLVPEGIEGRVPYKGSVVAIMFQQAGGLRAAMGYCGCA
                     TIDDMHARAEFVQITAAGIRESHVHDVQITKEAPNYRAD"
     gene            2524..4140
                     /gene="guaA"
                     /locus_tag="PKN51_08510"
     CDS             2524..4140
                     /gene="guaA"
                     /locus_tag="PKN51_08510"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNT85180.1"
                     /translation="MPHDKILILDFGSQVTQLIARRVREAHVYCEVHPCDVSSDWVRE
                     HAADGRLKGVILSGSHASVYEVDDKAPDAVFELGIPVLGICYGMQTMAQQLGGKVEGG
                     HTREFGYAEVRAHGHTPLLKDMADFTTPEGHGMLKVWMSHGDKVTALPPGFKLMASTP
                     SCPIAGMADEARGFYALQFHPEVTHTVQGRAMLERFVLQICGARPDWVMRDHIEEAVQ
                     AIRTQVGDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLHEGDMVMAMF
                     ADRLHARVVRVDASELFLNALRGVSDPEHKRKIIGRLFVDVFKAEAEKLKAGGAGHKG
                     ASFLAQGTIYPDVIESGGGKGKKAVVIKSHHNVGGLPEQLGLKLLEPLRDLFKDEVRE
                     LGLALGLPAEMVHRHPFPGPGLGVRILGEVKQEYADLLRHADAIFIEELRSTIEPHSG
                     KSWYDLTSQAFGVFLPVKSVGVMGDGRTYDHVVALRAVQTSDFMTADWAELPYELLKR
                     TSSRIINEVRGINRVTYDISSKPPATIEWE"
     gene            4313..4993
                     /locus_tag="PKN51_08515"
     CDS             4313..4993
                     /locus_tag="PKN51_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNT85181.1"
                     /translation="MYLPSQFATQDQRLAARVMREHPLASLITTDDAGLPYVSHLPLH
                     LQHEDTGFRLLGHMARPNVQWRHLQQHARALVTFMGPHAYMSPSVYPDRARVPSWNYV
                     AVHCSVTARLLDHDDAHAKDALLKCLIGDHEPAYAAQWRDMEGELARKLLAGIVAFEL
                     QVDHWQCKIKLNQHRPESHAALHAAYLGGGAQQQALADWMVRLGLVGGSSAPSGGPDD
                     PCDSNADS"
     gene            5052..6458
                     /gene="tadA"
                     /locus_tag="PKN51_08520"
     CDS             5052..6458
                     /gene="tadA"
                     /locus_tag="PKN51_08520"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNT85182.1"
                     /translation="MRVGEDRLTDDQAMRLALQQAQAAAALGEVPVGAVVVRDGRVIA
                     SGHNAPIGAHDPTAHAEVVALRAAAQALGNYRLDGCTLYVTLEPCAMCAGAMLHARLD
                     RVVFGASDPKAGAAGSVLDLFAETRLNHQTEVRGGVLAHECAALLRQFFKPRRINRDP
                     LRDDALRTPDTAFQDLPGHPWPPHYVSDLPALAGLRLHWVDEGPPDAPLTWLCLHGNP
                     GWSYLYRHMMAAFAAAGHRVVAPDLIGFGRSDKPKKEGTHRFDWHRQVLLEFIERLDL
                     QRIVLVVQDWGGLLGLTLPMVAPGRYLGLLAMNTMLATGDAPLPAGFIDWRQWCAERP
                     AYDIARLLARAEPSLSAAECAAYAAPFPDAGHRAATRAFPRLVPAHADQDGAAISRQA
                     RDFWRHQWRGHSLLVVGARDPVLGQPVMAELAALIRGCPPPWVLPEAGHFVPEHGRAI
                     AERALQHFGAARGSTAAL"
     gene            6522..7478
                     /locus_tag="PKN51_08525"
     CDS             6522..7478
                     /locus_tag="PKN51_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNT85183.1"
                     /translation="MAHIYIYSPSGAVRDRAAFRLGVRRLQAQGHEVEIDPTALASWQ
                     RFAGDDATRLAAIGRAAASGADVALMTRGGYGLSRLLPSLPYRQLARAIAKGTRFVGF
                     SDFTALQLALLGRTGAVTWAGPALCEGFGGAGTPGSAKGERAAPDDIMQACFDDLLSG
                     AGEGSGWRLPSSDVLALNGRQGALAQGAPLWGGNLTVLLSLLGTPWWPAVRHGVLFLE
                     DVGEHPYRIERMLAQLLHAGVLAQQKLIVLGQFTDYSLSAHDRGYSMARVVEWLRANL
                     KVPVLTGLPFGHVPTKVLLPVGQPVQALIEGRDALLLWGHLH"
     gene            complement(7547..7891)
                     /locus_tag="PKN51_08530"
     CDS             complement(7547..7891)
                     /locus_tag="PKN51_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85184.1"
                     /translation="MRWFKHGGLTALYERLSGSTPDPERMAYRVEDIRVAMLDMLGEQ
                     GEHSHPQVARRIRYAGDSLALWYARADLMAALAGLHGEQLARTRMVSLSVLFEGTLPK
                     GMASRPSSLSRF"
     gene            8162..9145
                     /locus_tag="PKN51_08535"
     CDS             8162..9145
                     /locus_tag="PKN51_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT85185.1"
                     /translation="MVFVDLADSTAAYAALGNAPVAEVVSKVTQWIGRVCDAHQGRIV
                     KYLGDGVLAQFADAQQAIEAVVFMQQSHTERLRKWPEPLHMGLKIGMACGPVVHKDGD
                     TYGDPVNLAARLSDLAGAHTIWADDTVITHLRQTHPRNSPVVDKVRYRSLGLIRVRGL
                     AQPQSVFQIEWNEELSSDLMTVRGALHDLSRDAQSSGASIALAWLGTSRVFSVSDMSV
                     TIGRTPDSSFVVSDQRVSRTHARIEFINGSFMLSDVSSFGSWVRFADKPEAEILLRRN
                     QCILHAAGEIALGAPFSDFSAATVAFDVAAGQLAPLGRAATRSLFGDSTQS"
     gene            complement(9176..9454)
                     /locus_tag="PKN51_08540"
     CDS             complement(9176..9454)
                     /locus_tag="PKN51_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85186.1"
                     /translation="MKAVEWVDRVKRVRSIESDYAAAKALGITRSAMSEIRNGHTLTL
                     KEETALRVASIIGERPEAIIIDQTAERVKEPATRAALFDAARRLCVLC"
     gene            9675..9893
                     /locus_tag="PKN51_08545"
     CDS             9675..9893
                     /locus_tag="PKN51_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85187.1"
                     /translation="MTDSTNIELHDFLGARWYVRRLGRDGSITVTSQQRRARCFAPGR
                     AAECAGRIRADFAALYTRADVVASEVAR"
     gene            9890..10285
                     /locus_tag="PKN51_08550"
     CDS             9890..10285
                     /locus_tag="PKN51_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85188.1"
                     /translation="MMSSVKVGGATISFYALGVDVHVLIRVGFMHFTRRLSVCQVASI
                     GAALALVLDSPPRQAAAHRVSLSDAGEWLEVARDGQGLPELSIFSPAWGAWRAGAKRD
                     TLAALAYALESAADEAACARIRAEIVPRP"
     gene            10406..10714
                     /locus_tag="PKN51_08555"
     CDS             10406..10714
                     /locus_tag="PKN51_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85189.1"
                     /translation="MILLNVLRDPLAVHLYLLLLAQMRYTEGHFLGTYARLMDLMTPP
                     APERGRRRGGPTYKRVRDALADLVEAGLVRRDERNESQGQLRLWLAARGTAKTKKKTA
                     "
     gene            11242..11406
                     /locus_tag="PKN51_08560"
     CDS             11242..11406
                     /locus_tag="PKN51_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85190.1"
                     /translation="MHVWQQVLFSAKGARKGQAGVVVKVNEADPSLVFVTWDADGATE
                     ECAAADLRVL"
     gene            11406..11717
                     /locus_tag="PKN51_08565"
     CDS             11406..11717
                     /locus_tag="PKN51_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85191.1"
                     /translation="MPLIGHTRCTDPECGAADIPVYRTSGGKVYAACHRCEMTQQPKP
                     GSAGHRRLMRRTVLVESEGDPPAPAAAPRAPADDGEPAAPPAIKNPQRMARVGFQLGT
                     L"
     gene            11714..12211
                     /locus_tag="PKN51_08570"
     CDS             11714..12211
                     /locus_tag="PKN51_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85192.1"
                     /translation="MTEPVNLELAQLARLDFDDADGGGALPPGGAEAGAAGEPGTAAL
                     DAAVSALALGILRTMRTLIARRLPEIKDEWTDDVLRAPADALPPVMRRYAGQVSEWAA
                     RFPELVALGVSAMPMAMGYMAAVERHARTVEDVQPKPAPAAPAPAAPAQGSNGGGGPA
                     VFHVG"
     gene            12201..12881
                     /locus_tag="PKN51_08575"
     CDS             12201..12881
                     /locus_tag="PKN51_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85193.1"
                     /translation="MSADKALTIAAFGARGTGKTAWIRQQLTAARPRRLMVWDFKHDA
                     ALSGLGREFSDWRAFVGACSKPEFQARYLVEQGKDIHQQFEAFCRVAWAAGCLVMFVD
                     ELPEVTRANRAPPAWRRCVNVGRDYDGGRKWLSIIAAGQRPTEVDKSFLSNCDVIHTG
                     RLAYAEDARLFARMWGIDPAEISNLPDLAWIEKQASRPAVERGTLNFSRRVAKKQQAS
                     NDPIGGGS"
     gene            12958..13122
                     /locus_tag="PKN51_08580"
     CDS             12958..13122
                     /locus_tag="PKN51_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85194.1"
                     /translation="MNLTTAQLTKLAIGLGICYAGYKFASNQAVKAMALGCAGVIIGR
                     QVPYLSDALA"
     gene            13134..13976
                     /locus_tag="PKN51_08585"
     CDS             13134..13976
                     /locus_tag="PKN51_08585"
                     /inference="COORDINATES: protein motif:HMM:NF037870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein P2"
                     /protein_id="HNT85195.1"
                     /translation="MNKQIILDPFSNVVATGVAVGDMNKFLGTVVEKITLDLGGTTFT
                     PSHITSIQLKLNGKVVWESSGARLVASNTYLGGATDNTKLKIDFMDRKAVTVNARQVG
                     SIDLSAGSGVTSARLEVSISGATAPTLGGFVDVSPPTADPAEAGIRPLIARRHAATVV
                     VGAAGTFALPIPHLDPAGGGSNYRRIYIYSANMTGLKTQREGVTEHELTKAQNEAAQK
                     DNGRVPQTNLFVFDPCQDGQLTGRTWDTRPVSGVRSAQLYATFSAGETITIETDELLP
                     IAAY"
     gene            13979..14260
                     /locus_tag="PKN51_08590"
     CDS             13979..14260
                     /locus_tag="PKN51_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85196.1"
                     /translation="MDPAEYTYTRTQDDLRPFRMFVGALQGALTADQSIYGTDPYAWN
                     MPGRYQVIGPTGVALEGRPIATTQNGGLYISPALVLMGLGAAAYFVLSK"
     gene            14257..14484
                     /locus_tag="PKN51_08595"
     CDS             14257..14484
                     /locus_tag="PKN51_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85197.1"
                     /translation="MNPSFSLGLPGLPPLSFGAPATMAQDFGGASWGASGAGDWNVNL
                     AGSGTALQTSTSGINWLLIAAGVLAWLVLKK"
     gene            14550..14870
                     /locus_tag="PKN51_08600"
     CDS             14550..14870
                     /locus_tag="PKN51_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85198.1"
                     /translation="MRPLVTAERVLTDATLLEYRHGAQRALLAVKGVGLPLGNRLDVV
                     ALVSDGERIESGQAIESLRRWAFEHHIDYITMMTARPHIARACARAGWKQTGVVMVGT
                     VNHV"
     gene            14863..15375
                     /locus_tag="PKN51_08605"
     CDS             14863..15375
                     /locus_tag="PKN51_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85199.1"
                     /translation="MSDTSNESNTTAHQTDNRRVIGQGGISSEHSNVTVVSNVLDGGA
                     WGYGRDVTGRVLDFGAEALNANNQATREALRQLSDVSESAMQSSLAAQQSTLAAARMA
                     MDSNADTLAAAFSLSGRTFTQAYDAIAGAGNMVATAYADAKGRGAKTDTMIMMAVAAM
                     GVVALMAVQK"
     gene            15372..15941
                     /locus_tag="PKN51_08610"
     CDS             15372..15941
                     /locus_tag="PKN51_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85200.1"
                     /translation="MKPTDIYTFTIGAGQAFNLKVAGEYFKILSATGAVDVRAEWGKL
                     SGLIAGQGLENTPFSGLEIRNVSGASNTLRVLIGDRNFIDGLSGSVSIGQAAPVRVGA
                     LTSNAKTVTSTSAQLAAAMAGRSFLLVQNNHASGSIFLNFGAAATLTGCIKVIPGGVF
                     ELGAGLVTTQDVHAIGDIASNASIVLVEG"
     gene            16259..16957
                     /locus_tag="PKN51_08615"
     CDS             16259..16957
                     /locus_tag="PKN51_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HNT85201.1"
                     /translation="MSIYLVAAALGVVLLARELARAGAPAGEGEALPPAGDVVAAFSD
                     LIGAGDQEASQLPDPFDSAAVILNPTTYRPDTSAPGEGDRNARAFLDMIAAAEGTAWQ
                     PDGGYRTLFGYGTFDSYADHPRVKVPFGRTYSTAAGRYQILARTWDGLVSKLGRAELP
                     DFGPDAQDRAALELIRERGALADVRSGRLADAVHKVRKVWASLPGANYAGQGMRSMAY
                     LEDSYRAAGGTTTT"
BASE COUNT         2929 a         5445 c         5743 g         2860 t
ORIGIN      
        1 cggaattcgc cgctcgcgag cagggctaca ccttcgtgtc gcaccagcag gaagtgggcg
       61 cgggttactt cgacgacgtg accaccgtga ttcagggcgg ctcctccagc gtgaaggccc
      121 tgacgggttc gaccgaggaa gagcagttcc actgatccgc gcttgaaggc cgaccggtgc
      181 gtgccggtcg gtgaggagac aggaaaagcc acggcatgcc gtggcttttt cgctcatggg
      241 cgaacccgaa ggcgctggca gagccggcgt cggcggcgac gttccgtctc gcggcttcac
      301 aggcggcggc gggcagtggt tgtgcccgtg ccagtttgct atcgaaaaga aagctcttga
      361 cgcagttccg atgagcctcg gggcttggtc atcgcatcca tgcgccgcct tgcagatacc
      421 agccgccgtg cggcgaccgg tgccagcggt gcggcacata gtggtgaccg gggcgggggc
      481 gttcatagcg accgggcacc cagacatggc ggtgcccgcc ccagcgccag tagccgccga
      541 tccacaccgc gccgggatac ggcagggccg gctgcacttc gtagtagggc gccggtggcg
      601 ccaccggcgc gtacaccgtg gtcgaggtga ccacaccgtc gcggtccgtg tagaccgcgc
      661 cgggcaccgg cgccaccatg caacccgcca gcgccgtggc ccctgccagg catccggcga
      721 gcagggcgcg gcgagaaggc gtgtggcttg tcagggtcat gtcggcaact ccttgaacag
      781 gctaaggcgg cagcctcgcg cctgttgcaa cgcgtggccc caccctggcg ccgacgcgcc
      841 tgcgtctgct cacctggctt tacctgacct tgacacgcgc ttccccctac gccctgcata
      901 tggaaagatg ctgcgtcggc gctgtttaca atccgtcttt tggagcctat cccatgcgcc
      961 tgatcggcca agcgctgacc ttcgacgatg tgttgctggt gccagcgtac tcccaggtcc
     1021 tgcccaagga cgtcaacctc tccacccagt tcacccgccg catccgcctg aacctgccgc
     1081 tggtgtctgc cgccatggac accgtcaccg aagcgcgcct ggccatcgcc atggcccagg
     1141 agggtggcat cggcatcgtg cacaagaatc tgacggcgca cgagcaggcc acgcaggtgg
     1201 ccaaggtcaa gcgttacgaa agcggtgtgc tgcgcgatcc ggtggtgatc acgcccgagc
     1261 acacggtgct gcaggtgatg cagctgtcgg acgaactgga catcagcggc tttcccgtca
     1321 tcgaccatgg ccgggtggtg ggcatcgtca ccgggcgcga tctgcggttt gaaacgcgct
     1381 acgacgtgcc ggtgcgcgaa atcatgaccc cgcgcaacaa gctgatcacc gtgcccgagg
     1441 gcaccaccct cgatgtcgcc aaggcactgt tgaacaagca ccgcctggag cgtctgctgg
     1501 tggtggatga gcaggaccgc ctgaagggcc tgatcacggt caaggacatc accaagcaga
     1561 ccacctttcc caacgccgcg cgcgacgccc atggccggct gcgcgtgggc gccgccgtgg
     1621 gcgtgggcga gggcaccgaa gagcgcgtcg aggcgctggt caaggccggt gtcgacgccc
     1681 tggtggtgga tacggcccat ggccacagcc agggtgtgat cgagcgcgtg cgctgggtga
     1741 agaacaagta tccgcaggtg gatgtgatcg gcggcaacat cgccaccggc aaggcggcgc
     1801 gcgcgctggt ggatgccggc gcagacggcg tcaaggtagg catcggcccg ggcagcatct
     1861 gcaccacccg catcgtcgcc ggcgtcggtg tgccacagat caccgccatc cacgacgtat
     1921 cgcaggcgct ggaaggcacc ggcgtgccca tgatcgccga cggcggcgtg cgctactcgg
     1981 gcgacatcgc caaggccatt gccgccggcg cgcacagcgt gatgatgggc ggcatgtttg
     2041 ccggtaccga agaggcgcct ggcgaaatcg tgctgttcca gggccgcagc tacaagagct
     2101 atcgcggcat gggcagcatc ggtgccatgc agcagggcag cgccgaccgc tattttcagg
     2161 aaagcagcac gggcaacccg aatgccgaca agctggtgcc cgaaggcatc gagggtcgcg
     2221 tgccctacaa gggcagcgtg gtggccatca tgttccagca ggcgggcggc ctgcgcgcgg
     2281 ccatgggata ttgcggctgc gccaccatcg atgacatgca cgccagggcg gaattcgtgc
     2341 agatcaccgc cgccggcatc cgcgaaagcc acgtgcacga cgtgcagatc accaaggaag
     2401 cgccgaacta tcgcgccgat tgagccggac gccgcaggcc atcggccggc cgcgcgctat
     2461 gttgttgata gctatcggcg ctggtccagt aagcgccaga agccaattta gcttcgaaac
     2521 gtcatgccac acgacaagat cctcatcctc gacttcggct cccaggtcac ccagctgatc
     2581 gcgcgccgcg tgcgcgaggc ccatgtgtac tgcgaggtcc acccctgcga cgtcagcagc
     2641 gactgggtgc gcgagcatgc cgccgacggc aggctcaagg gcgtgatcct ttcgggcagc
     2701 cacgccagcg tctacgaggt cgatgacaag gcgcccgacg ccgtgttcga gctgggcatt
     2761 ccggtgctgg gcatctgcta cggcatgcag accatggcgc agcagctggg cggcaaggtc
     2821 gaaggcggcc acacgcgcga gttcggctac gccgaggtgc gcgcgcatgg gcacacgccc
     2881 ctgctcaagg acatggccga cttcaccacg cccgaagggc acggcatgct gaaggtgtgg
     2941 atgagccacg gcgacaaggt gaccgccttg ccgcccggct tcaagctgat ggccagcacg
     3001 cccagttgcc ccatcgccgg catggccgac gaagcgcgtg gcttctatgc gcttcagttc
     3061 caccccgaag tcacccatac ggtgcagggc agggcgatgc tggagcgctt tgtccttcag
     3121 atctgtggcg cccggcccga ctgggtgatg cgcgatcaca tcgaggaggc cgtgcaggcg
     3181 attcggactc aggtgggtga cgaggaggtg atcctgggtc tgtccggcgg ggtcgattcg
     3241 agcgtcgccg ccgccttgat ccaccgcgcc atcggcgacc agctgacctg cgtgttcgtc
     3301 gaccacggcc tgctgcgcct gcacgaaggc gacatggtga tggccatgtt cgccgaccgc
     3361 ctgcacgccc gggtcgtgcg cgtggacgcc agcgagctgt tcctgaacgc gctgcggggc
     3421 gtcagcgatc cggagcacaa gcgcaagatc atcggccgcc tgttcgtcga cgtcttcaag
     3481 gccgaggctg aaaagctgaa agccggcggg gcagggcaca agggggcgag ctttctggcg
     3541 cagggcacga tctaccccga cgtcatcgaa tcgggtggcg gcaagggcaa gaaggcggtg
     3601 gtcatcaaga gccaccacaa cgtcggcggt ctgccggaac agctcggcct gaagctgctg
     3661 gagccgctgc gcgatctgtt caaggacgag gtgcgcgaac tgggcctggc gctgggcctg
     3721 cctgcggaga tggtgcatcg ccatccgttt ccggggcccg gcctgggggt gcgcatcctg
     3781 ggcgaagtga aacaggaata cgccgacctg ctgcgccacg ccgacgccat cttcatcgag
     3841 gagctgcgct ccaccatcga gccccactcg ggcaagagct ggtacgacct gaccagccag
     3901 gcgttcggcg tctttctgcc tgtcaagagc gtcggcgtga tgggcgatgg acgcacctac
     3961 gaccacgtgg tcgcgctgcg cgcggtgcag acctccgact tcatgaccgc cgattgggcc
     4021 gagctgccgt acgaactgct caagcgcacc agcagccgca tcatcaacga ggtgcgcggc
     4081 atcaaccgcg tcacctacga catatcttcc aagccgccgg cgacgatcga gtgggagtga
     4141 agaacatggt gtaggagcca tcgtcggtga tcgagatctc acctgggaat ggagctgcac
     4201 tccactcgta aggcaagggc ttctcggtct ggctcgctgg cgacaccatc agcgcaaccc
     4261 tcagacggtt acccaccggg ggttgtcagg gtcgattccc aagtcacagc gtatgtacct
     4321 gccctcgcaa ttcgccactc aagaccagcg cctggccgcc cgggtgatgc gtgagcaccc
     4381 gctggccagc ctgatcacga ccgacgacgc gggcctgccg tacgtgtcgc atctgccctt
     4441 gcatctgcag cacgaagaca cgggttttcg cctgctcggt cacatggccc ggcccaacgt
     4501 ccaatggcgc cacctgcagc agcatgcacg cgcgctggtc accttcatgg ggccgcacgc
     4561 ctacatgtcg cccagcgtct atccggaccg ggcgcgcgta cccagctgga actacgttgc
     4621 cgtgcattgc agcgtaaccg cgcgcctgct cgaccacgac gatgcgcacg ccaaggacgc
     4681 actgctgaaa tgcctgatcg gtgatcacga acctgcctat gccgcccaat ggcgcgacat
     4741 ggaaggcgaa ctggcgcgca agctgctggc cggtatcgtg gccttcgagc tgcaggtcga
     4801 tcactggcag tgcaagatca agctcaacca gcatcgtccg gaatcccatg cggcgctgca
     4861 cgccgcgtac cttggtggcg gcgcccagca gcaggcgctg gccgactgga tggtcaggct
     4921 cgggctggtg ggcggctcgt cggcaccgtc aggcggtccg gacgacccct gtgactcaaa
     4981 cgccgattcg tgaacgtttt tcagcatcag gccttgtaga tcatgcgcca gcagctacaa
     5041 aaacaggagt tattcgggtg ggcgaggacc gcctgaccga cgatcaggcc atgcgcctgg
     5101 cgctgcagca ggcgcaggcc gcggcggcgc tgggcgaggt gccggtgggc gctgtcgtgg
     5161 tcagggatgg tcgcgtcatc gccagcggcc acaacgcgcc catcggcgcg cacgatccga
     5221 ccgcccatgc cgaagtggtg gcgctgcggg cggcggcgca ggcgctgggc aactaccggc
     5281 tggacggctg cacgctgtac gtgacgctcg agccctgcgc catgtgtgcc ggcgcgatgc
     5341 tgcatgcccg gctggatcgc gtggtgtttg gcgccagcga ccccaaggcg ggcgctgcgg
     5401 ggtcggtgct ggacctgttt gccgaaacgc gccttaacca tcaaaccgag gtgcgcggcg
     5461 gcgtgctggc gcacgaatgc gcggcgctgc tgcgccagtt cttcaaaccg cggcggatca
     5521 accgcgaccc gctgcgtgac gacgccctgc gcacgcccga taccgccttc caagacttgc
     5581 ccggccaccc ctggccgccg cactacgtga gcgatctgcc agccctggcc gggctgcgcc
     5641 tgcactgggt ggacgaaggc ccgccggacg ccccgcttac ctggctgtgc ctgcacggca
     5701 acccgggctg gagctatctg tatcgccaca tgatggcggc atttgccgcc gccgggcacc
     5761 gcgtggtggc gcccgacctg atcggcttcg gccgcagcga caagcccaag aaggagggca
     5821 cgcaccgctt cgactggcat cgtcaggtgc tgctcgaatt catcgagcgg ctggacttgc
     5881 agcgcatcgt gctggtagtg caggactggg gcggcctgct ggggctgacg ctgccgatgg
     5941 tggcgcccgg gcgctacctg ggcctgctgg ccatgaacac catgctggcg acgggcgatg
     6001 cgccactgcc ggccggcttc atcgactggc gccagtggtg tgccgagcgt ccggcttacg
     6061 acatcgcgcg cctgctggcg cgtgccgaac cgtcgctcag cgcggccgaa tgtgccgcct
     6121 atgcggcccc gtttcccgat gccgggcacc gcgccgccac gcgcgccttc ccgcgcctgg
     6181 tgcccgcgca tgccgaccag gatggcgccg ccatctcgcg ccaagcgcgc gacttctggc
     6241 gccatcagtg gcgcgggcac agcctgctgg tggttggcgc gcgcgacccg gtgctgggtc
     6301 agcccgtgat ggcggagctg gcggcgctga tacgcggttg cccaccgccc tgggtgctcc
     6361 ctgaggcggg gcacttcgtg cccgagcacg ggcgcgccat cgctgaacgc gccctgcagc
     6421 acttcggcgc tgcccgagga tcgaccgcag ccctataatt ttgatagctg ccggcgcgcg
     6481 cctggaaagc gccgcaagcc gtttttttca gaagaccgat catggcacac atctacatct
     6541 attcgccctc cggcgccgtg cgtgaccgcg ccgccttccg ccttggcgtg cggcgcctgc
     6601 aggcgcaggg tcatgaggtg gagatcgacc cgacggcgct ggcgagttgg cagcgctttg
     6661 ccggcgacga cgccacgcgc ctggccgcca tcggccgtgc ggcggccagc ggcgccgacg
     6721 tggcgctgat gacgcgcggt ggctacggcc tgagccgcct gctgccgtct ttgccgtacc
     6781 gccagctcgc ccgggccatc gccaagggca cgcgcttcgt gggattcagc gacttcaccg
     6841 cactgcagct ggcgctgctg ggccgcaccg gcgccgtgac ctgggcgggc ccggcgctgt
     6901 gcgaaggctt cggcggcgcc ggcacgccgg gctcggccaa aggcgagcgg gcggcgccgg
     6961 acgacatcat gcaggcctgc ttcgacgatc tgctgtcggg tgcaggcgag ggcagtggct
     7021 ggcgcctgcc ttcctcggat gtcctggccc tcaacggccg gcaaggcgcg ctggcgcagg
     7081 gcgcgccgct gtggggtggc aacctgacgg tgctgctgtc gctgctgggc acgccgtggt
     7141 ggccggcggt caggcacggc gtgctgttcc tggaagacgt gggtgaacac ccgtatcgca
     7201 tcgagcgcat gctggcgcag ctgctgcacg cgggggtgct ggcgcagcag aagctgatcg
     7261 tgctgggcca gttcaccgac tacagcctga gcgcgcacga tcgcggctac tcgatggcca
     7321 gggtggtgga gtggctgcgc gccaacctga aggtgccggt gctgaccggc ttgcccttcg
     7381 gtcatgtgcc caccaaggtg ctgttgccgg tgggccagcc ggtgcaggcg ctgatcgagg
     7441 gcagggatgc gctgctgctg tgggggcact tgcattaagg gccagccagc atccaccggc
     7501 ccatggcaga ggcagcgggc tggccgttcg cgcgacggtg ggcgtatcag aagcgcgaca
     7561 gagaagaagg gcgcgatgcc atgcccttgg gcagggtgcc ttcgaacagg accgacagac
     7621 tgaccatgcg ggtgcgcgcc aactgctcgc cgtgcagacc ggccagggcc gccatcagat
     7681 cagcacgcgc ataccagagg gccagcgaat cgcccgcgta gcggatgcgg cgggccacct
     7741 gagggtggct gtgctcaccc tgttcaccca gcatatccag catcgccact cgaatgtctt
     7801 ccactcgata ggccatgcgc tcagggtcgg gcgtggagcc ggacaagcgc tcgtacaggg
     7861 cagtgagccc gccgtgcttg aaccatctca tcggcttgtt accaataggg gtttcggata
     7921 actgttggta accatactag tgcaaattcc ccaactgcgc cattggcgtt gttgaccagg
     7981 cctgaaaaga gaaaaaaata caacgcccaa atcggttcgc ctgccacgtg cgtcgggcgt
     8041 gcgacgctca cgtgctacca tttttacaat gcacggcatg ctgtgatcag ggtaaacacg
     8101 gaggagcagg gcgcccgatc accgaggcag ccaacctatt gctatgagcg ctccagtcac
     8161 catggttttc gtcgatctgg ccgacagcac ggctgcgtat gccgcgctgg gcaatgcccc
     8221 ggtcgccgaa gttgtctcca aggtcacgca gtggatcggg cgcgtctgtg atgcccacca
     8281 ggggcgcatc gtcaagtatc tgggcgatgg ggtgctggcg caatttgccg acgcgcaaca
     8341 ggcaatcgag gcggtggttt tcatgcagca aagccacacc gaacgcctgc gcaagtggcc
     8401 cgagccgctg cacatgggtc tgaagatcgg catggcctgc ggcccggtcg tgcacaagga
     8461 tggagacacc tacggtgacc cggtgaacct ggctgcccgc ctgtccgatc tggcaggggc
     8521 gcacacgatc tgggccgacg acacggtgat cacacacctg cgacagacgc atccccggaa
     8581 ctcgcccgtc gtcgacaagg tgcgctaccg ttcgctcggg ttgatacgcg tgcgcggtct
     8641 ggcccagccc cagtcggtgt ttcagatcga atggaacgaa gagctgtcgt ccgatctgat
     8701 gacggtgcgc ggggcgttgc acgatctttc gcgggatgcg cagtccagcg gcgccagcat
     8761 tgcgttggct tggctgggca cgtccagggt gttttcggtg tccgacatgt cggtcaccat
     8821 cgggcggact cccgacagca gtttcgtcgt cagcgatcag cgggtatcgc gcacgcacgc
     8881 gcgcatcgag ttcatcaacg gcagtttcat gctgtcggac gtcagcagct ttggctcctg
     8941 ggttcgcttc gccgacaagc cagaagctga gattctgctg cgtcgcaacc agtgcatcct
     9001 gcatgccgcc ggcgagatcg ccttgggcgc gccgttcagc gatttcagcg ccgccaccgt
     9061 ggcgttcgac gtcgccgcag gtcagctggc gccgctcggc agggcggcga cccgatcgct
     9121 gttcggcgat tcgacgcaga gctgagcaac cctgcgcttt gattttacct taatattaac
     9181 ataatacaca gaggcggcgc gcagcatcga aaagcgccgc gcgggtagcg ggttccttca
     9241 cccgttccgc agtctgatcg atgatgattg cctcgggccg ttccccgatg atcgatgcga
     9301 cccggagggc cgtttcttcc ttgagcgtga gcgtgtgccc gttgcggatt tcactcattg
     9361 ccgatcgagt gatgcccagg gctttggcgg cggcgtaatc gctttcgatc gaccgtaccc
     9421 gcttgactcg atcgacccat tcaactgcct tcataggtgg cgccctttca ggttgaggtg
     9481 cccgtatggt agttgcgcaa cactctacgt caagtgtcag gtttcacttg acgttccaca
     9541 cgtcatgtaa caccatacgc aggtctgccc gggctgactc cagttcttcc ctgaacccgg
     9601 caggttggcg cccgctggac ccgggcagac cccgcaaggg cgcgccggcc tacacacaca
     9661 agagggcgcc acccatgacc gattcgacga acatagagct gcatgacttc ctcggcgcgc
     9721 gctggtacgt gcgccgcctg gggcgtgacg gttcaatcac tgtcaccagt cagcagcggc
     9781 gcgcccggtg cttcgcgccc ggccgggccg ccgaatgcgc cggccgtata cgcgccgact
     9841 tcgcagcgct ttacacgcgc gccgatgtgg tggcttcgga ggtggcgcga tgatgtcgag
     9901 cgtgaaagtc ggtggagcga ccatcagttt ctatgcgctg ggcgttgatg ttcacgtatt
     9961 gatccgtgtc ggcttcatgc actttacgcg gcgtctttcg gtgtgccagg tggcaagcat
    10021 cggcgccgcg ctggcgctgg tgctggattc cccgccccgc caggcggcgg cgcatcgggt
    10081 gagcctgagc gacgccggcg agtggctgga agtggcgcgc gacggtcagg gcctgcctga
    10141 gctgtcgata ttcagtccgg cctggggggc atggcgcgcc ggtgcgaagc gcgacacgct
    10201 ggcggcgctg gcctacgcgc tcgaaagcgc agccgatgaa gcggcgtgcg cgcgtattcg
    10261 tgccgaaata gtgccccgtc cgtgatccgc gctgcacatg gcgcgcagtc aatacgggcc
    10321 ggccgctggt gcgccaggcg gcgcagaacc gcgtctggcg gcgcttcata ccccgcagtt
    10381 cctgcgcctt gaggcggttg agctggtgat tctgctgaac gttctacgcg acccgctggc
    10441 cgtgcatctt tacctgctgc tgctggcgca gatgcgctac acagaggggc attttctggg
    10501 cacctacgcg cgcctgatgg acctcatgac gccaccggcg ccagaacgcg gccggcgccg
    10561 cggcgggccg acatacaagc gggtgcgcga cgcgctggcc gacctggtag aggccggcct
    10621 ggtgcggcgc gacgagagga acgaatctca gggccagctg cgactgtggc tggccgcgcg
    10681 agggaccgca aagaccaaga aaaagaccgc ctgaaggcaa ccacacacac aacgcccgcg
    10741 aggcttgaga gcttcgcggg cgttgccgtt tctggcgcat taaagggcag ggtttagggc
    10801 agggtacacg gtagcgggtg gcatcttaga gcgcgcgcct agactgcgga gcatgctata
    10861 acaccgatag caaatggcgg aattagggca gggtataggg caggggtatc actgagtttt
    10921 tctttaacta ttacgcgcgc gtggagctgg gaaatccacc gacttgttca ccgacttatc
    10981 cacaacagcg cctacggcgc gcgcaacgca agcgcgcctg ctgcgcggcg cgcaccagta
    11041 gcggcgccgt gccgaaacgt gggcccgcgc gcacgcatgc gccgcttgct tgtcagtgaa
    11101 tcgtgattgg gcgaccgcgt gacgaatgca gcgcatagga agggctacaa gccatttttt
    11161 ttaagtgcct ggggggtagg gtgcagccgt gccaaaatag cgcgccaggg cgccacctgc
    11221 tgctacgtga ggagagcgag catgcatgtt tggcagcaag tcctgttttc ggctaaaggc
    11281 gcccgcaagg gtcaggccgg cgtggtggtg aaggtcaatg aagccgaccc ttccttggtg
    11341 ttcgtcacct gggacgccga cggcgccacc gaggaatgcg cagcggctga ccttcgggtg
    11401 ctgtaatgcc gctgatcggt cacacacgat gcaccgaccc ggaatgcggc gccgccgaca
    11461 ttccggttta ccgcacatcc gggggcaagg tgtacgcggc ctgccaccga tgcgaaatga
    11521 cgcagcaacc caagccgggc agcgcaggac atcggcgctt gatgcggcgc acggtgctgg
    11581 tggaatctga gggcgatccg ccggcgccgg ccgcagcgcc gcgcgccccg gccgacgatg
    11641 gcgagccggc cgcgccgccg gcgatcaaga acccgcagcg catggcgcgc gttggctttc
    11701 agttggggac actatgaccg agccggtaaa tcttgagctg gcgcagctgg cgcgcctgga
    11761 ctttgacgat gccgatggcg gcggcgcgct gccgccaggt ggcgcggagg ccggcgccgc
    11821 cggtgagcct ggaacggcgg cgcttgatgc ggcggtttcg gcgctggccc tgggcatcct
    11881 gcgcaccatg cggaccctga ttgcgcggcg cctgcccgaa atcaaggatg aatggaccga
    11941 cgatgtttta agggcgcccg ccgatgctct accgccggtc atgcgccgct acgccggcca
    12001 ggtgagcgag tgggccgccc gcttccctga gctggtggcg ctgggcgtgt cggccatgcc
    12061 gatggccatg gggtacatgg ccgcagtcga gcgacacgcg cgcaccgtgg aggatgtgca
    12121 gccgaaaccg gcgcccgcag cgccggcacc ggccgcgccg gcgcagggca gcaacggcgg
    12181 cggcgggccg gcggtgtttc atgtcggctg acaaggcgct tacgatcgcc gcattcggcg
    12241 cgcgcggcac cggaaagacc gcatggattc gccagcagct gacggcggcg cgcccgcgcc
    12301 gcttgatggt gtgggacttc aagcacgatg ccgcactgtc gggcctgggc cgtgagttca
    12361 gcgattggcg cgcgttcgtc ggcgcatgca gcaaaccgga atttcaggcg cgctacctgg
    12421 tggagcaggg caaggatatc caccagcagt tcgaggcctt ttgccgcgtg gcgtgggccg
    12481 ccggctgcct ggtcatgttc gtggacgagc tgcccgaggt cacgcgcgcg aaccgggcgc
    12541 cgccggcgtg gcgccggtgc gtgaatgtgg ggcgggacta tgacgggggc cgaaaatggc
    12601 tttcgatcat cgcggccggt cagcggccga ccgaggtgga taaatcgttt ctgtcgaatt
    12661 gcgacgtgat ccatacgggc cggctggcct acgctgaaga tgcccgactg ttcgcgcgca
    12721 tgtggggaat cgacccggct gaaatcagca accttcccga cctcgcctgg attgaaaagc
    12781 aagcgagccg gccggcggtg gagcgtggga cgctgaattt ttcgcgcaga gttgcaaaaa
    12841 aacaacaagc ctcgaatgac ccgataggcg gcggttcata ggcgcgccgt ttggcgtttt
    12901 tcgataacgg tggcaccgtg gcgccatccg tcgatcatca atcaaccctt ggaaatcatg
    12961 aatctgacga ctgcacaact tacgaaactg gcgatcggcc tgggcatttg ctatgccggc
    13021 tacaagttcg ccagcaatca ggccgttaag gcgatggcgc tgggctgcgc cggcgtcatc
    13081 atcggccgcc aggtgccgta cctgtccgac gcgctggcct gatcggggga cgcatgaata
    13141 aacagatcat tctggacccg ttcagcaacg ttgtggccac cggcgtggcg gtgggcgaca
    13201 tgaacaaatt tctcggcacc gtggtcgaga aaatcaccct tgacctgggc gggacgactt
    13261 tcaccccttc gcatatcacg tcgattcagt tgaagttgaa cgggaaggtc gtttgggaat
    13321 cgagcggcgc gcgcctggtg gcttcgaata cgtaccttgg cggggcgacg gacaacacga
    13381 aactcaaaat cgacttcatg gatcggaaag ccgtgacggt caatgcccgc caggtcggat
    13441 cgatcgatct cagtgccggc agcggcgtca cgtcggcgcg cctggaagtg tcgatttccg
    13501 gcgccacggc gccgacgctg ggcggcttcg ttgacgtgtc cccgccgacc gccgacccgg
    13561 ccgaggccgg cattcgaccg ctgatcgcgc gccggcatgc cgccaccgtg gtggtggggg
    13621 ccgccggcac cttcgcgctg ccgatccctc atcttgaccc ggccggcggc ggttcgaact
    13681 accgccgcat ctacatctac agcgccaaca tgacgggcct gaaaacccag cgcgaaggcg
    13741 tgacggaaca cgagctgacc aaggcccaga acgaggcggc gcagaaagat aacggccgcg
    13801 tcccccagac caacctgttc gttttcgacc cgtgccagga cgggcagctg accggccgca
    13861 cctgggacac gcgccccgtg tctggcgtgc gcagcgcgca gctgtacgcc acgttctcgg
    13921 ccggtgaaac catcacgatc gaaaccgatg agctgctgcc gatcgcggcc tactgagcat
    13981 ggacccggcc gagtacacct acacgcgaac gcaggatgat ctgcggccgt tccgaatgtt
    14041 cgtcggcgcg cttcaaggcg cgttgaccgc cgatcaaagc atttacggga ccgaccccta
    14101 cgcctggaac atgcccggcc gttatcaggt catcgggccg accggcgtgg cgttggaggg
    14161 ccgaccgatc gccaccacac agaacggcgg cttgtatatc tcgccggcgc tggtgttgat
    14221 gggcctgggc gcggccgctt acttcgtgct gagcaagtga atccgtcatt cagtctcggc
    14281 ctgcccggcc tgccgccgct gagcttcggc gcgcccgcga cgatggcgca agacttcggc
    14341 ggcgcgagct ggggcgcgag cggcgccggt gactggaacg tgaaccttgc agggtccggg
    14401 accgcgctgc aaacgtcaac gtccggtatc aattggctgc tgatcgcggc cggcgtgctg
    14461 gcgtggctgg tgctgaaaaa atgatgcggg cgattgactg gacagccggc cacgtcgatg
    14521 cgtttgcgcc atcggtgcag gcgtcaccga tgcgcccgct ggtgaccgct gagcgggtgc
    14581 tgaccgatgc gacgctgctg gaataccgcc acggcgcaca gcgcgcgctg ctggccgtca
    14641 agggcgtggg cctgccgctg ggcaatcggc ttgatgtggt ggcactggtg agcgacggcg
    14701 agcgcatcga atccggccag gccatcgaat cactgaggcg gtgggccttc gaacaccaca
    14761 tcgactacat caccatgatg acggcgcgcc cgcacatcgc gcgcgcctgc gcccgcgccg
    14821 gctggaagca gaccggcgtg gtcatggtcg gaactgtgaa ccatgtctga cacctcgaac
    14881 gagtccaaca ccacggcgca ccagaccgac aatcggcgcg tgatcgggca gggcggtatt
    14941 tcgtccgagc attcgaacgt gacggtggtc agcaatgtgc tggacggcgg cgcctggggg
    15001 tacgggcgcg acgttaccgg ccgcgtgctg gatttcggcg ccgaggcgct gaacgcgaac
    15061 aatcaagcga cgcgcgaggc gctgcgccag ctttcggacg tgtccgagtc ggccatgcaa
    15121 tcttcgctgg ccgcgcagca atcgacgctc gcggcggcac gcatggcgat ggactcgaac
    15181 gccgacacgc tggcggccgc cttcagtctg agcgggcgca cattcactca agcatacgac
    15241 gccatagcag gcgccgggaa catggttgcg accgcctacg ccgacgccaa ggggcgcgga
    15301 gcgaaaaccg acacgatgat tatgatggcc gtggcagcaa tgggcgtggt tgcattgatg
    15361 gcggtccaaa aatgaagccg accgacattt acacgttcac catcggcgcc ggccaggcgt
    15421 tcaatctcaa agtcgcgggc gaatacttca aaattctgtc agcgaccggc gccgttgatg
    15481 tgcgcgccga gtggggcaag ctgagcggct tgattgccgg gcagggtctg gaaaataccc
    15541 cattttctgg cctggaaatc aggaatgtat cgggcgcctc aaacacgctt cgcgtgttga
    15601 ttggcgatcg gaatttcatc gatggcctga gcggttccgt ttccattggc caggcggcgc
    15661 cagtgcgcgt gggcgcgctc acgtcgaacg caaaaaccgt caccagcacc agcgcccagc
    15721 tggcggcggc gatggctggt cgatcgtttc tgctggttca gaacaaccat gccagtggca
    15781 gcatctttct gaacttcggc gcggccgcga ccttgaccgg atgcatcaag gtgatccccg
    15841 gcggcgtgtt cgagctgggc gccggcctgg tgacaactca ggacgtgcat gcgatcgggg
    15901 acattgccag caatgcgagc atcgtcctgg tggagggctg attcatgctt tcacaaggaa
    15961 acgtcagcat cgggccgcag ggcatgaaat tgattgcctt cggcgctttc gataactctg
    16021 ggtcatggtt tctgaatggc tgcaccatga gcggaacttc tggacttcga acgttcacat
    16081 tttccgttgc aaggtcatcg acaaaatacc ttgtgataac catggtagac acaaaagata
    16141 tggcatgcag tgtcaccggg aaaacgaccg cgaaatttga tcttcagtcc tacaacgcgc
    16201 gaactgacac aaactcaggc gctatgtttt cgacgcattt tgaagtctgg gaatcgccat
    16261 gagcatttac ctggtagcgg ctgccctggg cgtggtgctg ctggcgcgcg agctggcgcg
    16321 cgccggtgcg ccggccggcg agggcgaggc gctgccgccg gccggcgacg tggtggccgc
    16381 tttttcggac ctgatcggcg ccggcgatca agaagcatcc cagctgcctg acccgttcga
    16441 ttctgctgcc gtgatcctca accccacgac ctaccgcccc gacacttccg cgccaggcga
    16501 aggcgaccgc aacgcgcgcg ctttccttga catgatcgcg gccgccgagg gcacggcctg
    16561 gcagcccgac ggcggctacc ggaccctgtt cggctatggc acgttcgata gctacgccga
    16621 ccatccccgc gtgaaagtgc cgttcggccg cacctacagc acggcggccg gccgatatca
    16681 aatcctcgcg cgcacctggg acgggctggt ttcaaagctg ggccgcgcag agctgcccga
    16741 cttcgggccg gatgcgcagg atcgcgccgc gcttgagctg atccgcgaac gcggcgcgct
    16801 ggccgatgtg cgatcgggcc ggctggcgga tgcggttcac aaggtccgga aagtctgggc
    16861 gagcctgccg ggcgcgaact acgccgggca gggcatgcgc agcatggcct atcttgagga
    16921 ttcctaccgc gccgccggcg ggactacaac gacctgaccc tatgagcaaa gacaccg
//
LOCUS       DAOQTZ010000062        16878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3876_length_16878_cov_13.782025, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000062 DAOQTZ010000000
VERSION     DAOQTZ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16878
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3876_length_16878_cov_13.782025"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKN51_08620"
     CDS             complement(<1..566)
                     /locus_tag="PKN51_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNT85202.1"
                     /translation="MSTQRRVVITGAGIVSCIGNDLDTVEASLRAGRSGIRAVPRFTE
                     MGLRSQVAGVPEIDLEARIDRKELRFMGDAAAYAQIALADAIAQAGLTPAQVSHPRTG
                     LIMGSGGGSPANQIEAADTLREKGIRRVGPYQVTRCMSSTVSACLATNFKVKGINYSI
                     TSACSTSAHCIGAAAQQIAWGMQDVMFAG"
     gene            complement(563..1075)
                     /gene="fabA"
                     /locus_tag="PKN51_08625"
     CDS             complement(563..1075)
                     /gene="fabA"
                     /locus_tag="PKN51_08625"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HNT85203.1"
                     /translation="MADSFSYDQLIASGEGRLFGPEAGRLPLPPMLMFDRITHIDDDG
                     GAHGLGRIEAELDVKPGLWFFACHFQGDPVMPGCLGLDAMWQLIGFYLTWLRLPGRGR
                     ALGAGEVKFTGEVGPDVKLVRYEIDIKRVIKRKLNMAIGDARLLADGKEIYVANDLRV
                     GLFTREGQAS"
     gene            complement(1131..2726)
                     /gene="lnt"
                     /locus_tag="PKN51_08630"
     CDS             complement(1131..2726)
                     /gene="lnt"
                     /locus_tag="PKN51_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNT85204.1"
                     /translation="MRRVHALASPVAALLAGVLQALSIADPWQGAPRWWLQTFSLAWL
                     VWQLVHQAERQSSWSASTWERPQTAPAWRRALALAGLFALGWLAGSFWWLFTSMHTYG
                     GLHPLLAAAAVLALAGFLALYYVVAGAAFVALAPTRRPVRAALLFAALWTLAELARGR
                     WLTGFPWGAGGYAQVDGPLAFLARYVGVYGVGFVGAALAALLALAPQVRWRQPRTLAG
                     TAALLALAGSLWGGRQMALDTPGAPALTSAVELLQGNIPQDQKFIPGTGMADALRWYG
                     AQLQASTAPLVVAPETAIPLLPRQLPEGYWEALRARYAQGPQAALLGVPLGDLEHGYT
                     NSVVGLAPGQAAPYRYDKHHLVPFGEFIPPLFRWFTELMRIPLGDFSRGAVGQLSFVW
                     QGQRLAPNICYEDLFGEELAARFIDPATAPTAFVNLSNIAWFGDGVAIDQHLAIGRMR
                     ALEFERPMLRATNTGATAIIDHRGQVTHRLARATRGVLRGQFEGRSHLTPYAAWAARL
                     GLWPLWLGCAALVVAALARQRRG"
     gene            complement(2726..3616)
                     /locus_tag="PKN51_08635"
     CDS             complement(2726..3616)
                     /locus_tag="PKN51_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNT85205.1"
                     /translation="MADPPSARNPERSNEREDKRSLFQKLIEFIRPGPDSTHELIETL
                     ADAEDKEVINAESRVMLEGVLRLSDMTAGDAMVAAPRMDLLDIDAPYEQLLRDVITTA
                     HSRFPVYQGERENIIGILLAKDLLKLQRAPELNLRTLLRPAMFIPESKGLSDLLRAFR
                     ISRNHLAMVVDEFGRTAGLITIEDVLEQIVGEIEDEFDIDADEGDIFALADHSWRVAG
                     DTPLERVNEAFGVRLGADPQGDLEAEFDTVGGLIAHEMGHVPKRGEHHRMGTLDFHVL
                     HTRGGAVRWFKVVRVPPTEA"
     gene            complement(3753..4448)
                     /locus_tag="PKN51_08640"
     CDS             complement(3753..4448)
                     /locus_tag="PKN51_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT85206.1"
                     /translation="MNQLHPPVIDTPIDPAPRRPSRRSAVVPIREVGPEHRERIARHL
                     LALDEHDRYLRFGYAAGDAQIRQYVDGLNFERDHVFGIFNRRLTLIAMAHLAYPNDFA
                     SAGFAEFGVSVAKHARGRGYGKRLFERAAIHAVNDGVQTLYIHALSENTAMLRIARQA
                     GAVVEHAGSESQAHLSLPPATFASRVSELLADQVGQVDFGLKTAASQARQLLATVQEV
                     RAAVRDGQHRSDL"
     assembly_gap    4552..4651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4792..5268)
                     /locus_tag="PKN51_08645"
     CDS             complement(4792..5268)
                     /locus_tag="PKN51_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNT85207.1"
                     /translation="MTAALDRLDRAILARLQTDGRETHEAIGQQVGLSASAVLRRIRR
                     LEEAGVIDRYVALLRPESLGLGLSAYVNVRLEKRAEHAKRNPMDEFRASVQGWPEVVE
                     CVALTGEMDFLLHLVVQDMGAYSRFMMDTLLRHPSVQDCKTSFVMDRVKTTTALPI"
     gene            5470..6597
                     /gene="hppD"
                     /locus_tag="PKN51_08650"
     CDS             5470..6597
                     /gene="hppD"
                     /locus_tag="PKN51_08650"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNT85208.1"
                     /translation="MPPASPAATTWDNPMGTDGFEFIEYAAPDPQAMGALFERMGFTA
                     VARHRHKNVTLYRQGEINFIINAEPDSFAQRFARLHGPSICAIAFRVQDAKAAYERAI
                     SLGAWGYAGQAGPGELNIPAIKGIGDSIIYLIDRWRGKNGAREGDLGNIGFYDVDFYP
                     LPGVQGGAAVNPEGYGMTVIDHLTHNVHKGRMDEWASFYERLFNFREIRYFDIEGQAT
                     GVKSKAMTSPCGKIRIPINEEGNEQKGQIQEYLDLYKGEGVQHIALGCKDIYHAVDQL
                     GLAGVKLLNTSDTYYELLPGRIPDLKEPIAELQARNILVDGHDGELLLQIFSENQLGP
                     IFFEFIQRKGNEGFGEGNFKALFETMELDQMRRGVLTQKPA"
     gene            complement(6776..7705)
                     /locus_tag="PKN51_08655"
     CDS             complement(6776..7705)
                     /locus_tag="PKN51_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNT85209.1"
                     /translation="MKRPLKAVLRALLGLALLAGVLTLADPARVLAQWRQADPAWLLA
                     GLLAAIGSNAVSALRWRALARWLGAELSAREAARWYFQAIGLNTLLPGAVVGGDLYRA
                     VMLRRAGQATAAAGWSVLLDRLSGLWMLCAIGALGAAACAPVLGPWLHLPPAPLAALL
                     LAGSALWLALPWALPALGRARPGSGGWLAPLRLAAARPDFRRQLLWQALASAAVQGLS
                     AAALAAGALALGVVQPLAVWAWAIAPVFLMAALPVSVGGWGTREAAAVAALAPFGVAP
                     AAAVGVGVIYGLYGLAQGALGALAFGLPRNATE"
     gene            complement(7702..8733)
                     /locus_tag="PKN51_08660"
     CDS             complement(7702..8733)
                     /locus_tag="PKN51_08660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT85210.1"
                     /translation="MSTDRSTAPDVSIVVPIYNEVDNLPDLVERIAAAMAGQALSFEL
                     LAVDDGSSDGSRARLRELAAGRPWLRPVLLARNYGQSSALQAGFDRVRGRYVVTLDAD
                     LQNEPGDIPLLLERLENDPEVDMVSGWRKDRQDAELSRKLPSRIANRLISRATGVHLH
                     DYGCALKAYRRPIIDRIRLYGELHRFIPSLAREAGARITEVPVRHHARTRGVSKYGID
                     RTFRVILDLILIVFFMRYRQRPLHAFGGLGLWLTAPGLLILGWLLLQKLLGEDIGGRP
                     LLLVGAMLVLMGAQLIAAGLIGELLIRVYHEAGGAPQFHATELRPDDEPKKAPAPVPP
                     APTATNKIA"
     gene            8816..10342
                     /locus_tag="PKN51_08665"
     CDS             8816..10342
                     /locus_tag="PKN51_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT85211.1"
                     /translation="MNLTAAPGVPLVRSPVTHPLAWLLALALAHVAVRVAISPALKWD
                     EAEQMLWSQQLALGYGAQPPLYTWLQWGVNRLVGPSVLALSLLKHALLALTYALMWLA
                     GRELLAPRGAWWAAASLMLLPTLGWYSVRDHTHTVLVTAMACGAWWLLLRIVRQPRPL
                     EFAALGLVCGLGMLAKYSFALVAGAMLLAALSEPQARRALLSRGWWWAPLVGLLVVLP
                     HLGWLLSHLSEATAGTLSKMEIDAARGPLKGARHLLQGVAGTLLLFALFALWAFGGAW
                     WRSPWQPALPWAQRVFGRYLLLVLVALLGMVLAGVTEFKGRWLLPLLCVVPLAAFAAR
                     PELQQHPRGARYTGAIVAMALLILLAAGLRPWFSGLRGEVDELNHPAAELGAALSAAG
                     YDGRSPIIAADHMMAGLLRTRFPRASVGSCTVKDDDPLSDCVAALVARARAAGQGWLL
                     VSRTDRLEPDWWALATARLPARPVRSIELPLRMVRDGTPPAHYQYLWQPAAPAPDQPQ
                     "
     gene            10353..11693
                     /locus_tag="PKN51_08670"
     CDS             10353..11693
                     /locus_tag="PKN51_08670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNT85212.1"
                     /translation="MKVTVFGTGYVGLVQGAVLADVGHHVLCIDVDEAKVAALRAGRI
                     PIHEPGLDALVRAGQAAGRLRFSTDAAEGVAHGELLFLAVGTPPDEDGSADLQHVLAV
                     ARSIATHMTVPKTLVNKSTVPVGTADRVAEVVRAVLRERGVQIAFDVVSNPEFLKEGA
                     AVADCQRPDRIVIGTASAAAEAQLRELYAPFNRNHDRMVVMDVKSAELTKYAANAMLA
                     TKISFINEIASLAERLGADVEAVRRGIGSDPRIGYHFIYPGMGYGGSCFPKDVQALIH
                     TAQSVGFEPELLQAVERRNQAQRQRLGQRIVAHYGGTLAGKTIAVWGLAFKPDTDDMR
                     EAPSRTLLAQLWAAGARVRAHDPVAMDEARRIFGARPDLVLCDSPSEALTGADALAIV
                     TEWKQFRVPDFDQLAQRLADRIVFDGRNLYEPALLARHGLAYESIGRPGVRPLT"
     gene            11818..12402
                     /locus_tag="PKN51_08675"
     CDS             11818..12402
                     /locus_tag="PKN51_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85213.1"
                     /translation="MPPPPAPDRRRACQRLLGAALLSPLLPALLACAGGASGGWLLPV
                     ARLDELLARRFPVTRSLAGLADLTLHSPRLSLLPAANRVATALDLVLTERLGGRRYTG
                     AIELDSALQLDLQQGAVRLSAVRVQRLGLDQLPPAAQTLLARHAPPVVEQLLQGLVIY
                     RLPPERLATLRAAGRGAPPQLRVQPDGLRLELAP"
     gene            12512..13276
                     /locus_tag="PKN51_08680"
     CDS             12512..13276
                     /locus_tag="PKN51_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT85214.1"
                     /translation="MPNTHLLIGQGAHKVIALHGWFGHARGWGPLTQHLNGSDFSYAF
                     MDQRGYGGMRGSGGPYTVEQIAQDALALADQLGWQRFSLIGHSMGGSAIQQVLVAAPA
                     RVRALVAVTPVSATGVPFDDQGWQLFTAAGSDPAARRGIIDFTTGNRLTGTWLDAMVA
                     STQQHSDDAAVAGYLLSWARTAFVEKIQGQQLPVLVLPGEHDPALGEAACRATWMQHY
                     PNAQLELVRNAGHYPMDEAPIILATQIEKFLATVPA"
     gene            complement(13392..14921)
                     /locus_tag="PKN51_08685"
     CDS             complement(13392..14921)
                     /locus_tag="PKN51_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNT85215.1"
                     /translation="MLSLGAAGAALLAGCALPPLPERTASRALDEGEALQTSLGRALA
                     PQVSRHPGRSGTHLLSDPLDAFAARMLLAHQAERSLDVQYYIWRGDHTGTMLLHELVR
                     AAERGVRVRLLLDDGGTGGLDRELAALAQYPRIEVRLFNPLLPRWLKPLGYLTDFARV
                     NRRMHNKSFTADNQASIVGGRNVGDEYFGATEGVVFADLDVLAVGPIVSEVSADFDRY
                     WASASSWPAQDVLPAADPSTVPALAARAGALTQSDAGRAYAQAAQRTPFMTDLLNQRL
                     ALQWSRVDMVSDDPAKGLAQAQRSGLLVDQLHRALGVPQRSVALVSPYFVPTNAGAEA
                     LIRLQQRGVKVLILTNAYEASDVPLVHAGYARHRTALLQAGVALYEMQRLAPAARGSL
                     VRPNRLGSSGSSLHAKVFAVDGQRAFVGSFNFDPRSALLNTELGFVIHSPELARQIEQ
                     TFDTEVPRSAYTLRLSESGQIEWLSAAEPPVLQRDEPGSTWWSRIMLGLLSLLPIDGL
                     L"
     gene            complement(15146..15571)
                     /locus_tag="PKN51_08690"
     CDS             complement(15146..15571)
                     /locus_tag="PKN51_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85216.1"
                     /translation="MQATLKPESEAGHDPCTQLLVRSWRLIMVRGVECPLVTHEFRCA
                     CGLDGREVLRAFCAFLCALAFSQRRRLWVNPPGEPRLTADETDMLRLIAAAQNGCAPL
                     LDAQLSGLARQPLRAALEHSVRELGALLQARQLRLPRPA"
     gene            complement(15654..16010)
                     /locus_tag="PKN51_08695"
                     /pseudo
     CDS             complement(15654..16010)
                     /locus_tag="PKN51_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
     gene            16085..16375
                     /locus_tag="PKN51_08700"
     CDS             16085..16375
                     /locus_tag="PKN51_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT85217.1"
                     /translation="MNRRPVLELLRRSKPELQARFGVTRLALFGSAARDGMHAGSDVD
                     VLVAFDGPATAPRYFGVQFYLEDLLGMPVDLVTEKALRPRLRPFVEKDLARV"
     gene            16368..16724
                     /locus_tag="PKN51_08705"
     CDS             16368..16724
                     /locus_tag="PKN51_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNT85218.1"
                     /translation="MSEPTQREWRFYIDDMIDFAGRVLAYTRDVDEGAFVANGLIYDA
                     TLRNLELIGEAATHVPDEVRHAHAEIPWRMLVATRNRLIHGYLGLDDDTLWSIVHDEV
                     PALLRQLEALKAASAR"
BASE COUNT         2508 a         6048 c         5865 g         2357 t
ORIGIN      
        1 ccggcgaaca tcacgtcctg catgccccag gcgatctgct gcgccgccgc gccgatgcag
       61 tgcgccgagg tggaacaggc cgaggtgatg gagtagttga tgcccttgac cttgaagttg
      121 gtggccaggc aggccgacac ggtggagctc atgcagcgcg tcacctgata cggcccgacg
      181 cggcggatgc ccttttcgcg cagcgtgtcg gcggcctcga tctggttggc cggcgagccg
      241 ccgcccgagc ccatgatgag cccggtgcgc gggtggctga cctgcgcggg ggtgaggccc
      301 gcctgggcga tggcgtccgc gagcgcgatc tgcgcgtagg cggcggcgtc gcccatgaag
      361 cgcagctcct tgcggtcgat gcgcgcttcc aggtcgatct cgggcacgcc ggccacctgg
      421 ctgcgcaggc ccatctcggt gaatctgggc accgcccgga tgcccgagcg cccggcgcgc
      481 agtgaggctt ccaccgtgtc cagatcgttg ccgatgcacg agacgatgcc cgcgccggtg
      541 atcacgacgc gccgctgggt gctcatgacg cttgcccctc ccgcgtgaac agacccacgc
      601 gcaggtcgtt ggcgacgtag atttccttgc cgtcggccag caggcgggcg tcgccgatgg
      661 ccatgttgag cttgcgcttg atgacgcgct tgatgtcgat ttcgtagcgc accagcttga
      721 cgtcggggcc gacctcgccg gtgaacttga cctcgccggc gcccagggcg cgcccgcgcc
      781 ccggcaggcg caaccaggtc aggtaaaagc cgatcagctg ccacatggca tcgagcccca
      841 ggcagccggg catcaccggg tcgccctgaa agtggcaggc gaagaaccac aggcccggct
      901 tgacgtccag ctcggcttcg atgcgtccca gcccatgcgc gccaccgtcg tcgtcgatgt
      961 gcgtgatgcg atcgaacatc agcatcggcg gcagcggcag gcgaccggcc tcgggcccga
     1021 acaggcggcc ctcccccgag gcaatcaact ggtcgtagga aaaagaatca gccatgccgc
     1081 gcattgtgcc tgagggctgc ggccgcgccc ggcgccgcgc cgctcgcgcc tcagccgcgg
     1141 cgttgccggg ccagggcggc caccaccagc gccgcgcagc ccagccacag cggccacagc
     1201 cccagacgcg cggcccaggc ggcgtagggc gtgaggtggc tgcggccttc gaactgtccg
     1261 cgcagcacgc cgcgcgtggc gcgcgccagc cggtgcgtca cctggccgcg gtggtcgatg
     1321 atggcggtgg cgccggtgtt ggtggcgcgc agcatggggc gctcgaactc cagcgcgcgc
     1381 atgcggccga tggccaggtg ctggtcgatc gccacgccgt cgccgaacca ggcgatgttg
     1441 ctcaggttga cgaaggccgt gggggccgtg gccgggtcga tgaagcgcgc cgccagctcc
     1501 tcgccgaaca gatcctcata gcagatgttg ggcgccagcc gctggccctg ccacacgaac
     1561 gacagttggc cgacggcgcc gcgcgagaaa tcgcccagcg ggatccgcat cagctcggtg
     1621 aaccagcgaa acagcggcgg aatgaactcg ccgaacggca ccagatggtg cttgtcgtag
     1681 cggtagggcg ccgcctgccc cggcgccagc ccgaccaccg agttggtgta gccatgctcc
     1741 agatcgccca gcggcacgcc cagcagcgcc gcctgcggcc cctgggcgta gcgcgcccgc
     1801 agcgcctccc agtagccctc gggcaactgg cgcggcagca gcggaatggc ggtttccggc
     1861 gccaccacca gcggtgccgt gctggcttgc aactgcgcgc cgtaccagcg cagcgcatcg
     1921 gccatgccgg tgccggggat gaacttctga tcctgcggga tgttgccctg cagcagctcg
     1981 accgcgctgg tcagcgccgg cgcgccgggc gtgtccagcg ccatctggcg cccgccccac
     2041 aggctgccgg ccagcgccag cagcgcagcg gtgcccgcca gggtgcgcgg ctggcgccag
     2101 cgcacctggg gcgccagcgc cagcagggcg gccagcgccg cgccgacgaa gccgaccccg
     2161 tacaccccca cgtagcgcgc cagaaacgcc agcggcccgt ccacctgggc gtagccgccc
     2221 gcgccccagg gaaagccggt cagccagcgg ccgcgcgcca gctcggccag ggtccacagc
     2281 gcggcgaaca gcagcgccgc gcgcaccggc cggcgcgtcg gcgccagcgc cacgaaggcg
     2341 gcgcccgcca cgacgtagta cagcgccaga aagcccgcca gcgccagcac cgccgccgcc
     2401 gccagcagcg ggtgcaggcc gccataggtg tgcatggagg tgaacagcca ccagaagctg
     2461 ccggccagcc agcccagggc gaacaggccg gccagcgcca gcgcgcggcg ccaggccggg
     2521 gcggtctgcg ggcgctccca ggtgctggcg ctccaggagc tctggcgctc ggcctgatgc
     2581 accagctgcc acaccagcca ggccaggctg aaggtctgca gccaccagcg cggcgcgccc
     2641 tgccaggggt cggcgatcga cagcgcctgc agcacgccgg ccagcagcgc cgccaccggc
     2701 gaggccaggg cgtgcacccg gcgcatcagg cttcggtcgg cggcacgcgc accaccttga
     2761 accagcgcac cgcgccgccg cgcgtgtgca gcacgtgaaa gtccagcgtg cccatgcggt
     2821 ggtgctcgcc gcgcttgggc acatggccca tttcgtgcgc gatcaggccg ccgacggtgt
     2881 cgaactcggc ctccaggtcg ccctgcgggt cggcgcccag gcgcacgccg aaggcttcgt
     2941 tgacgcgctc cagcggcgtg tcgccggcca cgcgccagct gtggtcggcc agggcgaaga
     3001 tgtcgccctc gtcggcgtcg atgtcgaact cgtcctcgat ctcgccgacg atctgctcca
     3061 gcacgtcctc gatggtgatc aggccggccg tgcggccgaa ctcgtcgacc accatggcca
     3121 ggtggttgcg gctgatgcga aacgcgcgca gcaggtcgct caggcccttg ctttcgggga
     3181 tgaacatggc cggccgcagc agtgtgcgca ggttcagctc gggcgcgcgc tggagcttga
     3241 gcaggtcctt ggccagcagg atgccgatga tgttctcgcg ctcgccctgg tagaccggaa
     3301 agcgcgaatg cgcggtggtg atgacgtcgc gcagcagctg ctcatagggc gcgtcgatgt
     3361 ccagcaggtc catgcgcggg gcggccacca tggcgtcgcc ggcggtcatg tcggacaggc
     3421 gcagcacgcc ttccagcatg acgcggcttt cggcgttgat gacctccttg tcctcggcgt
     3481 cggccagcgt ctcgatcagc tcgtgcgtcg agtcggggcc gggacggatg aactcgatca
     3541 gcttctgaaa caggctgcgc ttgtcctcgc gctcgttcga gcgttcgggg ttgcgcgcgg
     3601 aaggggggtc ggccacggtc gggtgcaggg cgtgctcaag ttgtgacaag ggcaaggata
     3661 gcccatgtgc cccgccgcgc cggcgcgcgc cggcaggcgc cgttcagggc gcggcgccgg
     3721 tcttcacggt acgcgctgaa cgcgcaccca cgtcacaggt ccgaccgatg ctggccgtcg
     3781 cgcaccgccg cgcgcacctc ctgcaccgtg gccagcagct ggcgcgcctg cgaggcggcg
     3841 gtcttcagac cgaaatcgac ctgaccgacc tgatcggcca gcagctcgct gacgcggctg
     3901 gcgaaggtgg ccggcggcag gctcaggtgg gcctgacttt cgctgccggc atgttcgacc
     3961 accgcaccgg cctggcgcgc gatgcgcagc atggcggtgt tctcgctcag ggcgtggatg
     4021 tacagggtct gcacgccgtc gttgacggcg tggatggcgg cgcgctcgaa caggcgcttg
     4081 ccgtagccgc gcccgcgggc gtgcttggcc accgagacgc cgaactcggc aaagccggcc
     4141 gacgcgaaat cgttcgggta ggccaggtgc gccatggcga tcagcgtcag gcggcggttg
     4201 aagatgccga acacgtggtc gcgctcgaag ttcagtccgt ccacgtactg gcggatctgg
     4261 gcgtcgcccg cagcgtaacc gaagcgcagg tagcggtcgt gctcgtccag cgccagcaga
     4321 tggcgtgcaa tgcgttcccg gtgctcgggc ccgacctcgc ggatcggcac cacggcgctc
     4381 cgtcgggacg ggcggcgcgg ggccgggtcg atcggggtgt cgatgacggg tgggtgaagc
     4441 tggttcatgc acccaatggt agggacgcgg ctctagagag aaaactccaa tcgtcaccct
     4501 gacgcgccga ctgtgtaacc gatcacgccg cagcgctgcg agacgcaaga annnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccctgacgc gccgactgtg taaccgatca
     4681 cgccgcagcg ctgcgagacg caagaatgtg cagcttctgg tgcaccgccg ttcatcctga
     4741 acctgtcgaa gggcttcgac aggttcagcc cgaacggcgt tcgacagcct cctagatcgg
     4801 cagcgccgtg gtggtcttga cacggtccat cacgaagctg gtcttgcagt cctgcaccga
     4861 ggggtgacgc agcagcgtgt ccatcatgaa gcgcgagtac gcccccatgt cctgcaccac
     4921 cagatgcaac agaaagtcca tctcgccggt cagggccacg cactccacca cctcgggcca
     4981 gccctgcacg ctggcgcgga attcgtccat cgggttgcgc ttggcgtgct cggcgcgctt
     5041 ttccagccgt acgttgacgt aggcgctcag ccccagcccg agcgattccg gccgcagcag
     5101 ggccacgtag cggtcgatca ccccggcctc ttccagccgc cggatgcgcc gcagcacggc
     5161 gctggccgac aacccgacct gctggccgat ggcctcgtgc gtttcgcgcc cgtcggtctg
     5221 caggcgtgcc aggatggcgc ggtcgaggcg gtcgagggcg gcggtcatgg ctgcggtggg
     5281 ctcagggtgg aaggacgata tcgggcgtga cgggaggatt cttgcatcaa tccggcgcga
     5341 aatgaccttg gtcaagcatt ttccttgcgc aacggcggga atggccggtg caactggctg
     5401 gaattcgcgc aggcgggcac tctacagttc atgatttgcc tgcccaccac ttctttcagg
     5461 agacaagcca tgccccccgc cagccccgcc gccacgacct gggacaaccc catgggtacc
     5521 gatggcttcg agttcatcga atacgccgct cccgatcctc aggccatggg cgcgctgttc
     5581 gagcgcatgg gttttaccgc cgtggcgcgc caccgccaca agaacgtcac cctgtaccgc
     5641 cagggcgaga tcaacttcat catcaacgcc gagcccgaca gctttgcgca gcgctttgcg
     5701 cgcctgcacg gacccagcat ctgcgccatc gcctttcgcg tgcaggacgc caaggcggcc
     5761 tacgagcgcg ccatcagcct gggcgcctgg ggctacgctg gccaggccgg cccgggtgag
     5821 ctgaacatcc cggccatcaa gggcatcggc gactccatca tctacctgat cgaccgctgg
     5881 cgcggcaaga acggtgcccg ggagggcgat ctcggcaaca tcggcttcta tgacgtcgat
     5941 ttctacccgc tgccgggcgt gcagggcggc gccgcagtca accccgaggg ctacggcatg
     6001 acggtgatcg atcacctcac gcacaacgtg cacaagggcc gcatggacga atgggccagc
     6061 ttctacgagc gcctgttcaa cttccgcgag atccgctact tcgacatcga gggccaggcc
     6121 accggcgtca agagcaaggc catgaccagc ccctgcggca agatccgcat cccgatcaac
     6181 gaagaaggca acgagcagaa gggccagatc caggagtacc tcgatctcta caagggcgag
     6241 ggtgtgcagc acatcgcgct gggctgcaag gacatctacc acgcggtcga ccagctcggc
     6301 ctggccggcg tcaagctgct gaacaccagc gacacctact acgagctgct gccggggcgc
     6361 atcccggacc tgaaggagcc gatcgccgag ctgcaggcgc gcaacatcct ggtcgacggc
     6421 cacgacggcg agctgctgct gcagatcttc agcgaaaacc agctcggccc gatcttcttc
     6481 gagttcatcc agcgcaaggg caacgaaggc tttggcgagg gcaacttcaa ggccctgttc
     6541 gagaccatgg agctcgacca gatgcgccgc ggcgtgctga cccagaagcc cgcctgaggc
     6601 cggcgccggc ggcgccgccg cgacaacccc tggacatccg tacgccgaag gcgcaagaaa
     6661 cattcattgg ctgattctgc gtgcgctgcg ttcgctatgg cgggcctcct gggcccgacc
     6721 caagtcagga ataaaatcgg gcgccagcgc ttgatggaca agcgccagca gctatttatt
     6781 ccgtagcatt tcgcggcagg ccgaaggcca gggcgcccag cgcgccctgg gccaggccgt
     6841 acaggccgta gatcaccccc acccccaccg ccgccgccgg cgccacgccg aacggcgcca
     6901 gcgccgccac cgccgccgcc tcgcgcgtgc cccagccgcc cacgctgacc ggcagcgccg
     6961 ccatcaggaa caccggcgcg atggcccagg cccacaccgc cagcggctgc accacgccca
     7021 gcgccagcgc gccggccgcc agcgccgccg ccgacagccc ctgcaccgcc gccgaggcca
     7081 gcgcctgcca cagcagctgg cggcgaaagt cgggccgcgc cgccgccagc cgcagcggcg
     7141 ccagccagcc gccgctgccc gggcgcgcgc gccccagcgc cggcaaggcc cagggcagcg
     7201 ccagccacag ggcgctgccg gccagcagca gcgccgccag cggcgccggc ggcaggtgca
     7261 gccagggccc cagcaccggc gcacaggcgg ccgcccccag agcgccgatg gcgcacagca
     7321 tccacagccc actcaggcgg tccagcagca ccgaccagcc ggccgccgcc gtcgcctgcc
     7381 cggcgcggcg cagcatcacg gcccggtaca gatcgccccc caccaccgcg cccggcagca
     7441 gggtgttcag gccgatggcc tggaagtacc agcgcgccgc ctcgcgcgcg ctcagctcgg
     7501 cgcccagcca gcgcgccagc gcgcgccagc gcagcgccga caccgcgttg gagccgatgg
     7561 ccgccagcag gcccgccagc agccaggccg gatcggcctg acgccactgc gccagcaccc
     7621 gcgccggatc ggccagcgtc agcaccccgg ccagcagcgc caggcccagc agggcccgca
     7681 gcacggcctt gagggggcgc ttcacgctat cttattagta gctgtcggcg caggtgggac
     7741 gggcgctgga gccttttttg gctcatcgtc cggacgcagc tcggtggcat ggaactgggg
     7801 cgccccgccg gcctcgtggt agacccggat cagcagctcg ccgatcagcc cggcggcgat
     7861 cagctgcgcc cccatcagca ccagcatcgc gcccaccagc agcagcgggc gcccgccgat
     7921 gtcctcgccc agcagctttt gcagcagcag ccagcccagg atcagcagcc cgggcgccgt
     7981 cagccacagg cccaggccgc cgaaggcgtg cagcgggcgc tggcggtagc gcatgaagaa
     8041 gacgatcagg atcaggtcca ggatgacgcg gaaggtgcgg tcgatgccgt acttggacac
     8101 cccgcgcgtg cgcgcatggt ggcgcaccgg cacctcggtg atgcgcgccc cggcctccct
     8161 ggccagcgaa gggatgaagc ggtgcagctc gccatacagg cggatgcggt cgatgatggg
     8221 gcggcggtag gccttgagcg cgcagccgta gtcgtgcagg tgcacgccgg tggcgcgcga
     8281 aatcaggcgg ttggcaatgc gcgagggcag tttgcgcgac agctcggcgt cctggcggtc
     8341 cttgcgccag ccgctcacca tgtccacctc ggggtcgttt tcgagccgtt cgagcagcag
     8401 cgggatgtcg cccggttcgt tctgcaggtc ggcgtccagc gtcaccacgt agcgcccgcg
     8461 cacgcggtcg aagccggcct gcagcgcgct cgactggccg tagttgcgcg ccagcagcac
     8521 cgggcgcagc caggggcggc cggcggccag ctcgcgcagg cgggcgcgac tgccgtcgct
     8581 ggagccatcg tccaccgcca gcagctcgaa gctgagcgcc tgcccggcca tggcggcggc
     8641 gatgcgctcg accagatcgg gcaggttgtc gacttcgttg tagatcggca ccacgatgga
     8701 cacatcgggc gccgtggagc ggtcggtgct catggggcgg gtgctggcgg gataatcgcg
     8761 cgattgtgcc ttgcgccgcc acggcgggtg acggcacggt gccaccccga ctccgatgaa
     8821 cctcaccgcc gcgcccggcg tgccgcttgt gcgctcgccc gtcacccatc ccctggcctg
     8881 gctgctggcg ctggcgctgg cgcacgtggc ggtgcgggtg gccatttcgc cggcgctcaa
     8941 gtgggacgag gccgagcaga tgctgtggtc gcagcaactg gcgctgggct acggcgcgca
     9001 gccgccgctc tacacctggc tgcaatgggg tgtcaatcgg ctggtcggcc ccagcgtgct
     9061 ggcgctgtcg ctgctcaagc acgcgctgct ggcgctgacc tacgccctca tgtggctggc
     9121 cgggcgcgag ctgctggcgc cgcgcggggc ctggtgggcc gccgccagcc tgatgctgct
     9181 gccgaccctg ggctggtatt cggtgcgcga ccacacccac accgtgctgg tcaccgccat
     9241 ggcctgcggt gcctggtggc tgctgctgcg catcgtgcgc cagccgcgcc cgctcgagtt
     9301 cgccgccctg ggcctggtgt gcggcctggg catgctggcc aagtacagct tcgccctggt
     9361 ggccggcgcc atgctgctgg ccgcgctgtc ggagccgcag gcgcggcgcg ccctgctgtc
     9421 gcgcggctgg tggtgggcgc cgctggtggg gctgctggtg gtgctgccgc acctgggctg
     9481 gctgctgtcg cacctgtccg aggccaccgc cggcaccctg agcaagatgg agatcgacgc
     9541 cgcccgcggc ccgctcaagg gcgcgcgcca cctgctgcag ggcgtggccg gcaccctgct
     9601 gctgttcgcg ctgttcgcgc tgtgggcgtt tggtggcgcc tggtggcgct cgccgtggca
     9661 gccggccctg ccctgggcgc agcgggtgtt cggccgctac ctgctgctgg tgctggtggc
     9721 gctgctgggc atggtgctgg ccggggtgac cgagttcaag ggccgctggc tgctgccgct
     9781 gctgtgcgtg gtgccgctgg cggcgttcgc ggcgcggccc gagctgcagc agcacccgcg
     9841 cggcgcgcgc tacaccggcg ccatcgtcgc catggcgctg ctgatcctgc tggcggccgg
     9901 tctgcggccc tggttcagcg gcctgcgcgg cgaggtcgac gagctcaacc acccggcggc
     9961 cgaactgggc gccgccctca gcgccgccgg ctacgacggc cgcagcccca tcattgccgc
    10021 cgaccacatg atggccggcc tgctgcgcac gcgctttccg cgcgcctcgg tcggctcctg
    10081 caccgtcaag gacgacgacc cgctgagcga ctgcgtggcg gccctggtgg cgcgtgcccg
    10141 ggcggccggc cagggctggc tgctggtgtc gcgcaccgac cggctggagc ccgactggtg
    10201 ggcgctggcc acggcgcgcc tgccggcgcg gccggtgcgc agcatcgaac tgcccttgcg
    10261 catggtgcgc gacggcacgc cgccggcgca ttaccagtac ctgtggcagc cggccgcgcc
    10321 ggcccccgac caacctcagt gagcccaagc ccatgaaagt caccgtattc ggcaccggct
    10381 acgtcggcct ggtgcaaggc gccgtgctgg ccgacgtcgg ccaccatgtg ctgtgcatcg
    10441 acgtcgacga ggccaaggtg gcggcgctgc gcgccggccg catcccgatc cacgaacccg
    10501 gcctggacgc cctggtgcgg gccggccagg cggccgggcg gctgcgcttt tccaccgacg
    10561 ccgccgaggg cgtggcgcat ggcgagctgc tgttcctggc cgttggcacc ccgcccgacg
    10621 aagacggcag cgccgacctg cagcatgtgc tggcggtggc gcgcagcatc gccacccaca
    10681 tgacggtgcc caagaccctg gtcaacaagt ccaccgtgcc ggtgggcacc gccgaccgcg
    10741 tggccgaggt ggtgcgcgcc gtgctgcgcg agcgcggggt gcagatcgcc ttcgacgtcg
    10801 tctccaaccc cgaattcctg aaggaaggcg cggccgtggc cgactgccag cggcccgacc
    10861 gcatcgtcat cggcaccgca agcgccgccg ccgaggcgca gttgcgcgag ctgtacgcgc
    10921 ccttcaaccg caaccacgac cgcatggtgg tgatggacgt gaaaagcgcc gagctgacca
    10981 agtacgccgc caacgccatg ctggccacca agatcagctt catcaacgaa atcgccagcc
    11041 tggccgagcg cctgggcgcc gacgtcgagg cggtgcgccg cggcatcggc agcgacccgc
    11101 gcatcggcta ccacttcatc taccccggca tgggctacgg cggcagctgc tttcccaagg
    11161 acgtgcaggc gctgatccac accgcgcagt cggtcggctt cgagcccgag ctgctgcagg
    11221 cggtggagcg gcgcaaccag gcgcagcgcc agcggctcgg ccagcgcatc gtggcgcact
    11281 acggtggcac gctggcgggc aagaccatcg ccgtctgggg cctggccttc aagcccgaca
    11341 ccgacgacat gcgcgaggcg ccaagccgca ccctgctggc ccagctgtgg gccgccggcg
    11401 cccgggtgcg cgcgcacgac ccggtggcca tggacgaggc gcgccgcatc ttcggcgccc
    11461 ggcccgacct ggtgctgtgc gacagcccgt ccgaggcgct gaccggcgcc gatgcgctgg
    11521 ccatcgtcac cgagtggaag cagtttcgcg tgcccgactt cgaccagctg gcacagcggt
    11581 tggccgaccg catcgtgttc gacggccgca acctgtacga accggccctg ctggcgcgcc
    11641 acggcctggc ttacgaatcc atcggccggc ccggcgtgcg gccgctcacc tgagcttcca
    11701 tgactatcga ttggatagct tttggcgcag gatggataag ccccaacggc cgattgggcg
    11761 cttaaccctg ggtgtcggca gtcgcctgcc gccggcgctt cgggtatctt gtgcgccatg
    11821 ccaccgccac ccgccccaga ccgtcgccgc gcctgccagc gcctgctcgg cgcggccctg
    11881 ctgagcccgc tgctgccggc cctgctggcc tgcgccggcg gcgcgtccgg cggctggctg
    11941 ctgcccgtcg cgcggctgga cgagctgctg gcgcggcgct ttccggtcac ccgcagcctg
    12001 gccggcctgg ccgatctgac cctgcactcg ccgcgcctga gcctgctgcc ggcggccaac
    12061 cgcgtggcca ccgcgctcga tctggtgctg accgagcgcc tgggcgggcg ccgctacacc
    12121 ggcgccatcg agctcgacag cgccctgcag ctcgacctgc agcagggcgc cgtgcgcctg
    12181 agcgcggtgc gcgtgcagcg cctcggcctc gaccagctgc cgcccgccgc gcagacgctg
    12241 ctggcgcgcc acgccccgcc cgtggtcgag cagctgctgc aagggctggt catctaccgc
    12301 ctgccgcccg aacgcctggc caccctgcgc gcggccgggc gcggcgcgcc gccccagctg
    12361 cgcgtgcagc ccgacggcct gcgcctggag ctggcgccct gagccgcggc gcacccatcg
    12421 cgccggcccg cggagaccgc ccggccgtgc ctgcgacctt cgtatgatga tcgcagcacc
    12481 tcatacccac caccacccaa ggagacatgc catgcccaac acccacctgc tgatcggcca
    12541 gggcgcgcac aaggtcatcg cgctgcacgg ctggttcggc cacgcgcgcg gctggggccc
    12601 gctgacccag cacctgaacg gcagcgactt cagctacgcc ttcatggacc agcgcggcta
    12661 cggcggcatg cgcggctcgg gcgggcccta caccgtcgag cagatcgccc aggacgcgct
    12721 ggcgctggcc gaccagctgg gctggcagcg tttttcgctg atcggccatt cgatgggcgg
    12781 cagcgccatc cagcaggtgc tggtggccgc gcccgcgcgc gtgcgtgcgc tggtggccgt
    12841 cacccccgtc tcggccaccg gcgtgccgtt cgatgaccag ggctggcaac tgttcaccgc
    12901 cgccggcagc gacccggcgg cgcgccgcgg catcatcgac ttcaccaccg gcaaccgcct
    12961 caccggcacc tggctggacg ccatggtggc cagcacgcag cagcattcgg acgacgcggc
    13021 ggtggccggc tacctgctgt cctgggcccg gaccgccttc gtcgagaaga tccagggcca
    13081 gcagctgccg gtgctggtgc tgcccggtga acacgacccc gcactgggcg aagccgcctg
    13141 ccgcgccacc tggatgcagc actacccgaa cgcgcagctc gagctggtgc gcaacgccgg
    13201 ccactacccg atggacgagg cacccatcat cctggccacg cagatcgaga aattcctcgc
    13261 cacggtgccg gcgtagtcgc cgcgcgaact atcaaaaaca tagctgccgg cgctgactgg
    13321 atgggcgctg gatgccgatt gggctcggat cgtgcgggtt ccggcgatgc gcgccatggc
    13381 gcgcggcgac cctacagcag cccgtcgatc ggcagcagcg acagcaggcc cagcatgatg
    13441 cggctccacc aggtgctgcc cggttcgtcg cgctggagca ccggcggctc agcggcgctc
    13501 agccactcga tctgcccgct ctcggacagg cgcagggtgt aggcgctgcg cggcacctcg
    13561 gtgtcgaaag tctgttcgat ctggcgcgcc agctcggggc tgtggatgac gaagcccagc
    13621 tcggtgttga gcagcgccga gcgcgggtcg aagttgaacg agccgacgaa ggcgcgctgg
    13681 ccgtcgaccg cgaagacctt ggcgtgcagg ctcgagcccg agctgcccag ccgattcgga
    13741 cgcacgagcg agccccgggc cgccggcgcg aggcgctgca tctcgtacag cgccacgccg
    13801 gcctgcagca gcgccgtgcg gtgcctggcg tagccggcgt gcaccagggg cacgtcgctg
    13861 gcctcgtagg cgttggtcag gatgagcacc ttcacgccgc gctgctgcag acgtatcaag
    13921 gcctcggcgc ccgcgttcgt gggcacgaaa tacggtgaca ccagggccac cgagcgctgc
    13981 ggcacgccca gcgcgcggtg cagttgatcg accagcaggc cgctgcgctg cgcctgcgcc
    14041 aggcccttgg ccggatcgtc gctcaccatg tccacgcggc tccattgcag cgccagccgc
    14101 tggttcagca gatcggtcat gaagggcgtg cgctgcgccg cctgggcata ggcgcggccg
    14161 gcgtcggact gggtcagcgc gcctgcacgg gcggccagcg cgggcacggt gctcggatcg
    14221 gccgccggca ggacgtcctg ggcgggccag gacgatgcgc tggcccagta gcggtcgaag
    14281 tcggccgaca cctcggacac gatgggaccg accgccagca catccagatc ggcaaacacc
    14341 acgccctcgg tggcaccgaa gtattcatcg cccacgttgc gcccgcccac gatgctggcc
    14401 tggttgtcgg cggtgaacga cttgttgtgc atgcgccggt tgacgcgggc gaagtcggtc
    14461 aggtagccca gcggcttgag ccagcgcggc aggagaggat tgaacagccg cacctcgatg
    14521 cggggatact gcgccagcgc cgccagttcg cggtccaggc cgccggtgcc gccgtcgtcc
    14581 agcagcaggc gcacccgcac cccgcgttcg gccgcgcgga ccagctcgtg cagcagcatg
    14641 gtgccggtgt ggtcgccgcg ccagatgtag tactgcacgt cgaggctgcg ctcggcctgg
    14701 tgggccagca gcatgcgcgc cgcgaaggca tcgagcgggt cggacagcag gtgggtgccg
    14761 ctgcggcccg ggtgccgcga gacctgcggg gccagggcgc ggcccagtga agtctgcagc
    14821 gcctcgccct catcgagcgc gcgtgatgcc gtccgctcgg gaagcggtgg cagggcgcag
    14881 ccggccagca aggcggcgcc ggccgcgccc agggacagca agcgtagaag gggtgacacc
    14941 ggtgggcccg agcagggggt tcatggtaac ccgtgcgccc aagccatcct caacggccat
    15001 gggtgcaaac ccgcgccatg gcggagctat ggctgtggtc tgcgcctcat cccggccaaa
    15061 agtccagcgg tcttatgggt tccctcaagc acgcaacacc acaccaggag gtcgggcgcc
    15121 gctggctggc tgcgcctgga cggcctcagg ccggccgcgg caggcgcagc tggcgcgcct
    15181 gcagcagggc gcccagctcg cgcacgctgt gctccagcgc ggcgcgcagc ggctggcgtg
    15241 ccagcccgct caactgggcg tcgagcagcg gcgcgcagcc gttctgcgcc gccgcgatca
    15301 gcctcagcat gtcggtttca tcggcggtca gccgcggctc gcccggcggg ttgacccaca
    15361 ggcggcgccg ctgcgaaaac gccagcgcgc acagaaaggc gcagaaagcg cgcaacacct
    15421 cgcgcccgtc caggccgcag gcgcagcgga attcgtgcgt caccagcggg cattcgacgc
    15481 cgcgcaccat gatcagccgc cacgagcgca ccagcagctg cgtgcagggg tcgtgacccg
    15541 cctcggattc cggtttcagg gttgcctgca tgtgccgtgc ctcgcggtcg ttggtatggg
    15601 gcacgactat acatgcgaat aattctcatt tgcaagccct gcgccgggtg acgtcaggcc
    15661 gccgggactt gcaggccccg ctgcaccgcc ggccgggcga cgaaggcgtc cagcacgcgc
    15721 cgcacctgga caaagcgctc gaagccgacc agctcgcccg cctcgtaaaa acccaccagg
    15781 ttgcgcaccc aggggaagat ggcgatatcg gcgatgctgt agtcgctgcc cagcacccag
    15841 tcgcggccct gcagctggcg ctccagcacg cccagcaggc gcacgctttc ggccacgtag
    15901 cggtcgcgcg ggcgcttgtc ctcgaagtcc ttgccggcga acttgtggaa gaagccgagc
    15961 tggccgaaca tggggccggc gccgcccatt tgccacatca gccactgcag ggcgtgccag
    16021 cgcgtggcca cggccgcctc gatttgcgag aatttgaagc caaacctcct tcagaagcag
    16081 cgacatgaac cgacgacctg tactcgaact gctccggcgc agcaagcccg aactgcaggc
    16141 gcgctttggc gtgaccaggt tggccttgtt cggctctgcc gcgcgggatg ggatgcacgc
    16201 tggcagcgat gtggacgtgc tggtcgcctt tgatggcccc gccacggcgc cgcgctattt
    16261 cggcgtgcag ttctatctgg aggatctgct gggcatgccc gttgatctgg tgaccgagaa
    16321 ggcgttgcgg ccacggttgc gtccgttcgt cgagaaggat ctggcccgtg tctgagccga
    16381 ctcagcgtga atggcggttc tatatcgacg acatgatcga cttcgcgggg cgcgtgctcg
    16441 cctatacgcg ggatgtggac gagggggctt tcgtcgccaa tggcctgatc tacgacgcaa
    16501 ccttgcgcaa tctcgagctg attggcgagg cagccacgca tgtcccggac gaggtgcgtc
    16561 atgcgcatgc cgagattccg tggcggatgc tcgtggcgac gcgcaatcga ctgattcacg
    16621 ggtatctcgg ccttgacgat gacacgctct ggagcatcgt gcacgacgag gtgcccgcct
    16681 tgctgcggca attggaggct ctgaaggccg ccagcgccag atagaccgtc agtgctgcgg
    16741 gggggcacac actcacgcag tgccagatcg tcccggcgcc gccgccgcac cccgaaggtc
    16801 accccgccgg gacttgcagg ccgcgctgca ccgccggccg ggcgacgaag gcgtccagca
    16861 cgcgccgcac ctggacaa
//
LOCUS       DAOQTZ010000063        16754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_3906_length_16754_cov_13.545781, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000063 DAOQTZ010000000
VERSION     DAOQTZ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16754
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3906_length_16754_cov_13.545781"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKN51_08710"
     CDS             complement(<1..404)
                     /locus_tag="PKN51_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT85219.1"
                     /translation="MQDTYILTLSCPDQRGIVHAVSGFLMERGGNIEEAAQFNDHGTG
                     LFFMRVQFACTELDGAALRAQLPTLAERFAMRWQLHEAAQPMRTVIFVSREGHCLNDL
                     LFRWKSGLLPLDIRAIVSNHRDFYQLAASYNVP"
     gene            517..2124
                     /locus_tag="PKN51_08715"
     CDS             517..2124
                     /locus_tag="PKN51_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT85220.1"
                     /translation="MTAPEQPLHEHLRRHARQRPDAAAVIWYGRAISWRELDDWSDAL
                     GAHLQSLGVAPGQPVALFMNNCPQYLLAHYAIQKIGAIVCPCGPLNKAHELQYQLEDL
                     QARVILAADNLLPIVDQVRAQTALAHVLAVRYGDLLPAAPTLAVPTELQAAPAPLPAA
                     VGDLLQIAQQGAAQGQRPRPLAIGLGDVALMTYTSGTTGLPKGAMLSYGNALFKTQVT
                     AQSAGIGAGDVALSVAPLYHIAGMLMGVNVPVLAGATQVLLYRFDPLAVLQAIDRYRV
                     THWYSIAPMNVACMQVPDAGRFDLGSLRRNPVTSFGITFTQALAEQWQAFTRGPCLSY
                     EAAYGLSETHTMDTGMPPEAIRWGTHGQPMPGVSIRIVDPDSGVEKPVGETGEITVRS
                     PGNFKGYWNKPEASARTLRDGWVWTGDMGRMDAEGYLTFIGRTKEMIKVSGYSVFPEE
                     VESLLIRHPAVAQAAVLGEPDPDKGEVVKAFIVLKPGASLTADALIAWSRDNMAPYKA
                     PRHVRFIDALPATGAGKVLRRLLKELD"
     gene            2257..5547
                     /locus_tag="PKN51_08720"
     CDS             2257..5547
                     /locus_tag="PKN51_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNT85221.1"
                     /translation="MFHKVLIANRGEIALRIVRALQDLGLPSLVVYADDDAQAPHVRS
                     ADQAVALGASGPAAYLDGARLLAIARTHGADALHPGYGFLAERADFARDCAAAGVRFI
                     GPTPEQLALCGDKAQARALAERCGVPLLPGTQQAVTLAQAQAFFEQHAQAGIVIKAVA
                     GGGGRGMRAIEHAAELPAAYARCRSEALAAFGVADVYVERLMRRARHIEVQVLGDGHD
                     VMALGERECTLQRRFQKLVEIAPSPSLPAPLRQRLVDSALRMARELRYQGLGTFEFLV
                     DAASDTLPYVFIEANARLQVEHTITEAVTGVDLVQTQLRLAAGQRLADAGLDVAAPPP
                     PRGHAIQWRINAETLAADGSATPGSGRLTRFELPAGPGVRVDTHGVAGATPSPHYDSL
                     LAKLIVHAPGSDFAEVLRRSRRALADTRIDGVATNLALLRALCRRPEMASQQVHTRWL
                     EAALPELLDAAKTIAAAADQESSAGQNDAQTPQAVEAAEAPQGALLAPMPARLVQLSV
                     AVGDAVAAGAELAVLEAMKMEHVLTAPHAGVLRQCFVAPGAYVAQGDVLLVLEPADVS
                     DAAQQAAAAAHDPDHIRADLQRVLERHAFTLDAARPEAVARRHARGGRTVRENIADLC
                     DEGSFIEYGALAVAAQTRRRSMADLIANTPADGMVTGIGGVNGLIFGAEAARTAVMGY
                     DATVLAGTQGMRNHAKTDRLLGIALDQQLPVVLLAEGGGGRPADVDVPVVAGLHVGTF
                     AAYAALSGRVPVVGVVHGYCFAGNAALLGCSDVIIATRASHIGMGGPAMIEGGGLGVF
                     APEQIGPSGVQHGNGVIDLLVDDEAAAVAAARHYLSYFQGRVRDWVTPDQRLLRAVVP
                     ENRLRVYDSRDAMAGLVDQGSLLALRTGFGAGVHTALARIEGRPVGLIANNPLHLGGA
                     IDAAAADKAARFMQLCNAHGLPIVSLIDTPGFMVGPEVEQTAQVRHVSRLFVTAASLR
                     VPFFSVVLRKGYGLGAMAMAAGGFHAPVFTLSWPTGEFGGMGLEGYVRLGFRKELEAA
                     AEGAERDALFARLLAERYEHGAALNMATTLEIDAVIDPAETRAWLARGLASARLRPAT
                     GSRFVDTW"
     gene            complement(5621..6436)
                     /locus_tag="PKN51_08725"
     CDS             complement(5621..6436)
                     /locus_tag="PKN51_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNT85222.1"
                     /translation="MRRLILLATLVLAACALPPLDAGQQARLATLLPTDALLLGEQHD
                     APEHQHLQRQVVQWLGDRGQLAAVVLEMAEGGRSTAGLPRDARAHQVRAALGWDDARW
                     PWQVYGPVIMRAVAAGAPVQGANLSPAQMREAMRQSALDARLPPALLAVQRQRIRASH
                     CGLLPERQIDPMTRVQIARDIAMADAVAGAARQAAPGRVVLLITGNGHVHRALGVPRF
                     LPPSLKSKVLSAQAEQAQAAPENEALAGTALQPGDLLWPTPALPPRDPCTTLR"
     gene            6468..7550
                     /locus_tag="PKN51_08730"
     CDS             6468..7550
                     /locus_tag="PKN51_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HNT85223.1"
                     /translation="MPSSAVPPFVRAALTLLLAWGAARLGVWLSLPVPWMLGPLVATA
                     LASMAGLPTHSVRALRNAGQWVIGVSLGLYFTPAMAALMLGHWWALLLGVLWALALGL
                     GFAAWLRRATRASLGELSPAERAATLHFASTIGGASEMTLLAERAGARTDLVAAAHSV
                     RMAVVVLSLPFAMQWARQHWQLPALDVLPAAERVVDGPGLLWLALATGAGAALLRRTG
                     GANPWFIGPLAVAMALTMLDRHLSAVPPALGQAAQLVIGVSLGVRFSRRFLHAAPRWL
                     AAVALGTLGMIALCAAMAWVLAWATGLHPVTLMLATSPGGLAEMAITAQVLQLGAPVV
                     TAFQVCRLVAVLLLAEPLFRWGSARR"
     gene            complement(7562..7843)
                     /locus_tag="PKN51_08735"
     CDS             complement(7562..7843)
                     /locus_tag="PKN51_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HNT85224.1"
                     /translation="MNTLYDSDSFAVVHLLPDVLADTPAQAGQPVLARHGFEIVDKRS
                     GKEVYLDGLWAELFQQRIAAWQQNTPTQEEVEETLEGYAELAQNPVVMH"
     gene            complement(7976..9013)
                     /locus_tag="PKN51_08740"
     CDS             complement(7976..9013)
                     /locus_tag="PKN51_08740"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNT85225.1"
                     /translation="MSRQPPPHRARPPLALVGAVLLAHVLLLRPAPPRATPAPAPRAA
                     PLWLRAIPAPAATPAVTPVASAGATPSAPGRATSPAGTARTPAVTAPSPRRLTPRARA
                     SAAAPPPRTETDGRQTPAVPAAPAVAVAAPVRLADTVTLHYRVSGLARGLPLQADAQL
                     RWQREGSHYEAEWTLQLPPIGTRRQHSTGRVTPAGLVPERYAERQRGERAAHFDAEGG
                     RIRFSANTPDTTLQAGAQDRLSTLLQLGGLIAADPPRYPPGSQIVLQTAGAREAADWR
                     WEVLDDEVLQLDGQAVPCVRLQRAPRHEYDTRIELWLARALHYLPARLRVTQASGDVA
                     DQQLSSLPTPR"
     gene            9130..10146
                     /locus_tag="PKN51_08745"
     CDS             9130..10146
                     /locus_tag="PKN51_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNT85226.1"
                     /translation="MKLATYKDGSRDGQLVVVSRDLTTAHYATGIASRLQQALDDWNF
                     IAPQLQDLYDALNGGRARHPFAFEPERCMAPLPRAYQWLDGSAYLNHVELVRAAGRAD
                     VPERFYTEPLMYQGGSDDLLGPCDDIRVADEAFGIDFEGELAVVTGDVPMGATPEQGL
                     EAVRLLMLANDVSLRQLIAAEVETRLGLVQGKPSTAFSPVAVTPDELGEAWQDGRVHL
                     TLQVSWNGRKVGLCEAGPDMRFHFGQLIAHAARTRRLRAGSIVGSGTVSNPGVDQGGR
                     RDWPKGYGCIAEKRAMETLQDGAPRTDYMRFGDTVRIEMKGRDGRPLFGAIDQKVVQQ
                     GSGA"
     gene            10330..11127
                     /locus_tag="PKN51_08750"
     CDS             10330..11127
                     /locus_tag="PKN51_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT85227.1"
                     /translation="MNTPTLHTLRLDIDGDAVRATLCRPERLNAVGAQLLDELVVLAD
                     WLGEREDLHYLVLDHEGPVFSAGANLEEARELLADAGRARARLRANQRAAQQALIKLA
                     ALEQISFAALRGSAYGAGVAIAMACDFRVMAEDAVLNLPETRLGMFLSYGATPPLVAA
                     LGLARAKEMILFAEDWDAARCLAAGAVQQVAARGQVGARIDAMIATLRQRHWPAVRLA
                     KQVANAAAPARWGEVTLTEPELAAEALAGGDVAARVEAFLRRHHPER"
     gene            complement(11216..11818)
                     /locus_tag="PKN51_08755"
     CDS             complement(11216..11818)
                     /locus_tag="PKN51_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNT85228.1"
                     /translation="MNDSFASATILLLLITDPVGNLPIFTHALRAVPPTRRRRVILRE
                     VLIAFALLLVFMFLGDGFLRLMNLSPLALQLAGGVVLFLIALRMVFPSAQPPDEPAPT
                     EPLIVPLAIPALAGPSALATVMLLVSQAPERRWEWVGALSVTMAVCALVLLLAERLQQ
                     RLGPRVLMAFERLMGLILVAVAVEMLLRAFRTLAAQLGGG"
     gene            12192..13028
                     /locus_tag="PKN51_08760"
     CDS             12192..13028
                     /locus_tag="PKN51_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT85229.1"
                     /translation="MHAWPTALFVLLWSSGAIVSRWGLDHASPFILLTMRFALALLVL
                     AALGWRTRAWLPAPGTAAHVAAVGLLLVGGYSCSYFLALDQGLTPGALATMLGVQPIL
                     TLLLQERRVTPMRLLGLLLALGGLVLVVKDSLLRAQVSLAGIALALLALACITLGAMA
                     QQRLAQSPIRVLPLQCAVALLLCLALVPTQPLRLEVGLPLLACVLWLGAVISVGATVL
                     LYRMLQAGNLVNVTSLFYLVPGGTLLLDAWLLGHPVTPLALGGLALVVGGLALVLRTR
                     PA"
     gene            13084..13893
                     /locus_tag="PKN51_08765"
     CDS             13084..13893
                     /locus_tag="PKN51_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT85230.1"
                     /translation="MSQPAIDYSRYQTFRISRRGEGGCVLDLQMKAANGKLPTAGHEG
                     HWELAEIWRDIDKDDSVRAVVLRGEGMGFSGGGDLALVQDMASDFAVRSRVWKEARDL
                     VYNVIHCEKPIVSAMHGPAVGAGLVAGLLADISIAARSARIVDGHTRLGVAAGDHAAI
                     CWPLLCGMAKAKYYLMLCEAVTGEEAERIGLVSLAVDDDQLLAKAYEVADKLAAGSTT
                     AIRWTKYSLNNWYRQAGPSFDTSLALEFMGFGGPDVHEGVASLRERRPPRY"
     gene            complement(13970..14620)
                     /locus_tag="PKN51_08770"
     CDS             complement(13970..14620)
                     /locus_tag="PKN51_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNT85231.1"
                     /translation="MLKLCGFAASNYYNKVKLALLEKGVPFEEVTAWVGETDTTASPL
                     GKVPYLLTDDGPISESTVILEYLEQRYPETPLLPASPYQAAKVRELLRYIELHLELVA
                     RNLYPQAFFGGQVSDGLKQRIRPQLERHVAAFARLAKFGPYIAGEQFTLADCAAAVHL
                     PLVSGASKIIYGQDLLAALPVKDYLQRLNQRPAVQRVEAERRANIALMMARMQPKT"
     gene            14746..15108
                     /locus_tag="PKN51_08775"
     CDS             14746..15108
                     /locus_tag="PKN51_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85232.1"
                     /translation="MNPLRHAHRLARQVLVWFVLALVVAVAAPLVRAQPFELLCGAGG
                     QIKAVVHTASGDTLELGAGHLDCPLCVPTGAPGPLPRLGLAGPAPACVPDPTAPALRL
                     APAPLPWQARAPPPTHFR"
     gene            15274..15741
                     /locus_tag="PKN51_08780"
     CDS             15274..15741
                     /locus_tag="PKN51_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNT85233.1"
                     /translation="MKRLAISLSALALCAAASAQVTITEPWVRGTVPQQKASGLFMRI
                     DAEQHVRLLGGSSPVAGVVEVHEMAMQGEVMKMRALSHGLEIAKGHSLVLKPGGYHVM
                     LMELKQQLKAGDRVSVTLTFEDVASKQRFTREVQAPVTALGGAQPAPAAHHRH"
     gene            15838..16677
                     /locus_tag="PKN51_08785"
     CDS             15838..16677
                     /locus_tag="PKN51_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85234.1"
                     /translation="MSDSSNFDFGKLIPGFDFLQNLATSAKGVAAGPVSALRNWVAPT
                     VSVEELDKRITELKAVLFWLEQNSTALKATIQALEVQKMTLATLAGMNLSMSEVAKAF
                     TVGSPAKPDAAAASAPAAPPHWPFGQAPAAAPAAEPEPEPEPEPEPPARARAPARKSK
                     AKATPAATAAAPAPALADPMQWWGALTQQFQQIAANAVREAARAVPEQPAAAPAATAA
                     AATAKKPAARKAPAARKAARKPAAKKAASRAKAATEAAPPATETRGATLGWPLPPPFK
                     LGR"
BASE COUNT         2298 a         5880 c         6139 g         2437 t
ORIGIN      
        1 ggcacgttgt agctggccgc cagctggtag aagtcgcggt ggttgctgac gatggcgcgg
       61 atgtccagcg gcagcaggcc gctcttccag cggaacagca gatcgttcag gcaatggccc
      121 tcgcggctga cgaagatcac ggtgcgcatg ggctgcgccg cctcgtgcag ctgccagcgc
      181 atggcaaagc gctcggccag cgtcggcagc tgggcgcgca gcgcggcgcc gtccagctcg
      241 gtgcaggcga actgcacccg catgaagaac aggccggtgc cgtggtcgtt gaactgcgcg
      301 gcctcctcga tgttgccgcc gcgctccatc agaaagcccg agacggcgtg cacgatgccg
      361 cgctggtccg ggcaggacag ggtcaggatg taggtgtctt gcatcgggcg cgattgtcgc
      421 aggcgacgcg ctgctgtcac ctgcgcgatc gttcacgggc ggccaggtgc ccccggctcg
      481 ctatattggt tttttgcctc attcatggag acctgcatga ccgcccccga gcagcccctg
      541 cacgagcacc tgcgccgcca cgcgcggcag cggcccgacg ccgccgccgt gatctggtat
      601 ggccgcgcca tcagctggcg cgagctggac gactggagcg acgcgctggg cgcccacctg
      661 cagtcgctgg gcgtggcgcc gggccagccg gtggcgctgt tcatgaacaa ctgcccgcag
      721 tacctgctgg cgcactacgc gatccagaag atcggcgcca tcgtctgccc ctgcgggcca
      781 ctgaacaagg cgcacgagct gcaataccag ctggaggatc tgcaggcacg cgtcatcctg
      841 gccgccgaca acctgctgcc catcgtcgat caggtgcgcg cgcagaccgc actggcgcat
      901 gtgctggcgg tgcgctatgg cgatctgctg ccggccgcgc ccaccctggc cgtgccgacc
      961 gagctgcagg ccgcgccggc cccgctgccg gccgcggtgg gcgatttgct gcagattgcg
     1021 cagcagggcg cggcgcaggg ccagcgcccg cggccgctgg ccatcgggct gggcgacgtg
     1081 gccctgatga cctacaccag cggcaccacc ggtctgccca agggcgccat gctgagctac
     1141 ggcaacgcgc tgttcaagac ccaggtcacc gcccagtcgg ccggcatcgg cgccggcgat
     1201 gtggcgctgt cggtggcgcc gctctaccac atcgccggca tgctgatggg cgtcaatgtg
     1261 ccggtgctgg ccggcgccac ccaggtgctg ctgtaccgct tcgacccgct ggcggtgctg
     1321 caggcgatcg accgctatcg ggtcacgcac tggtattcga tcgcgcccat gaacgtggcc
     1381 tgcatgcagg tgcccgatgc gggccgcttc gacctgggca gcctgcggcg caacccggtc
     1441 accagtttcg gcatcacctt cacgcaggcg ctggccgagc agtggcaggc cttcacccgg
     1501 ggcccgtgcc tgagctacga agccgcctac gggctgtcgg aaacccacac catggacacc
     1561 ggcatgccgc ccgaggcgat ccgctggggc acccacggcc agcccatgcc gggcgtcagc
     1621 atccgcatcg tcgaccccga cagcggcgtt gaaaagccgg tcggcgaaac cggcgaaatc
     1681 acggtgcgct cgccaggcaa cttcaagggc tactggaaca agcccgaagc cagcgccagg
     1741 acgctgcgcg acggctgggt ctggaccggc gacatgggcc gcatggacgc cgagggctac
     1801 ctgaccttca tcggccgcac caaggagatg atcaaggtgt cgggctacag cgtgttcccg
     1861 gaagaggtgg aatccctgct gatccgccac cctgcggtgg cgcaggcggc ggtgctgggc
     1921 gagcccgacc ccgacaaggg cgaggtggtc aaggccttca tcgtgctcaa gcccggcgcc
     1981 agcctgacgg ccgacgcgct gatcgcctgg agccgcgaca acatggcccc ctacaaggcg
     2041 ccgcgccatg tgcgcttcat cgacgcgctg ccggccaccg gcgcgggcaa ggtgctgcgg
     2101 cggctgctga aagagctgga ctgaagacag acctccgtac gcagagggcg cagaggttgc
     2161 gcagaggacg caaaaccatt cctcgaagtt ggttctgcgc cttctgcatg atctttgcgt
     2221 cctctgcgta tggatgttca tgcatccccc cacaccatgt tccacaaagt tctgatcgcc
     2281 aaccgcggcg aaattgccct gcgcatcgtg cgcgccttgc aggatctggg cctgcccagc
     2341 ctggtcgtgt atgccgacga cgacgcgcag gcgccgcatg tgcggtcggc cgaccaggcg
     2401 gtggcgctgg gcgccagcgg tccggcggcc tatctggacg gcgcgcgcct gctggccatc
     2461 gcgcgcacgc acggcgccga cgccctgcac cccggctacg gctttctggc cgaacgggcc
     2521 gacttcgcgc gcgactgcgc ggcggccggc gtgcgcttca tcggccccac gcccgagcag
     2581 ctggcgctgt gcggcgacaa ggcgcaggcg cgcgccctgg ccgagcgctg cggcgtgccc
     2641 ctgctgcccg gcacccagca ggcggtgacg ctggcgcagg cgcaggcgtt ctttgagcag
     2701 catgcgcagg ccggcatcgt catcaaggcg gtggccggcg gtggcgggcg cggcatgcgc
     2761 gccatcgaac acgcggccga gctgcccgcc gcctacgcgc gctgccgcag cgaggcgctg
     2821 gcggccttcg gtgtcgccga cgtgtatgtg gagcgcctga tgcgccgcgc gcgccacatc
     2881 gaggtgcagg tgctgggcga cggccacgac gtgatggcgc tgggcgagcg cgaatgcacg
     2941 ctgcagcggc gcttccagaa gctggtcgaa atcgccccca gccccagcct gccggcgccg
     3001 ctgcgccagc gcctcgtcga cagcgcgctg cgcatggcgc gcgagctgcg ctaccagggc
     3061 ctgggcacgt tcgaattcct ggtcgatgcg gcatcggaca cgctgcccta tgtgttcatc
     3121 gaggccaacg cgcgcctgca ggtcgagcac accatcaccg aggcggtgac cggggtcgac
     3181 ctggtgcaga cccagctgcg gctggccgcc ggccagcgcc tggccgacgc cgggctggac
     3241 gtcgccgcgc caccgccgcc gcgtggccac gccatccagt ggcgcatcaa cgccgagacc
     3301 ctggccgccg acggcagcgc caccccgggc agcggccggc tgacgcgctt cgagctgccg
     3361 gccggccccg gcgtgcgggt ggacacgcac ggcgtggccg gtgccacgcc ctcgccgcac
     3421 tacgacagcc tgctggccaa gctgatcgtg cacgcgcccg gcagcgactt tgccgaggtg
     3481 ctgcgccgct cgcgccgcgc gctggccgac acccgcatcg acggcgtggc caccaacctg
     3541 gcgctgctgc gcgcgctttg ccgtaggccc gaaatggcca gccagcaagt gcacacgcgc
     3601 tggctcgaag cggccctgcc ggaactcctg gatgctgcaa aaacaatagc tgctgccgca
     3661 gaccaggaaa gctctgcagg ccaaaatgat gcccaaaccc cgcaagcggt ggaggccgcc
     3721 gaggcaccgc aaggcgcgct gctggcgccg atgccggcgc gcctggtgca gctgtcggtg
     3781 gcggtcggcg atgcggtggc ggccggcgcc gaactggcgg tgctggaagc catgaagatg
     3841 gagcatgtgc tcaccgcgcc ccacgccggc gtgctgcgcc agtgcttcgt cgcgcccggc
     3901 gcctacgtgg cgcagggcga cgtgctgctg gtgctggaac cggccgacgt gagcgacgcc
     3961 gcgcagcagg ccgcggccgc cgcccacgac cccgaccaca tccgcgccga cctgcagcgc
     4021 gtgctggagc gccacgcctt cacgctggac gccgcgcgcc ccgaggcggt ggcgcggcgc
     4081 cacgcgcgcg gcgggcgcac cgtgcgcgag aacatcgccg acctgtgcga cgaaggcagc
     4141 ttcatcgaat acggcgcgct ggcggtggcg gcgcagacgc gccggcgcag catggccgac
     4201 ctgattgcca acaccccggc cgacggcatg gtgaccggca tcggcggcgt caacggcctg
     4261 atcttcggcg ccgaggccgc gcgcaccgcg gtgatgggct acgacgccac cgtgctggcc
     4321 ggcacgcagg gcatgcgcaa ccatgccaag accgaccgcc tgctgggcat cgcgctcgat
     4381 cagcaactgc cggtggtgct gctggccgaa ggcggcggcg gccggccggc cgacgtcgac
     4441 gtgccggtgg tggccggcct gcacgtgggc accttcgccg cctacgccgc gctgtcgggc
     4501 cgggtgccgg tggtcggggt ggtgcacggc tactgctttg ccggcaacgc cgccctgctg
     4561 ggctgcagcg acgtcatcat cgccacccgc gccagccaca tcggcatggg tgggccggcc
     4621 atgatcgaag gcggcggcct gggcgtgttc gcgcccgagc agatcggccc cagcggcgtg
     4681 cagcacggca acggcgtgat cgatctgctg gtcgatgacg aagcggccgc ggtggcggcg
     4741 gcgcggcact acctgtcgta ctttcagggc cgggtcaggg actgggtcac gcccgatcag
     4801 cggctgctgc gcgcggtggt gccggaaaac cggctgcgcg tgtacgacag ccgcgacgcc
     4861 atggccggcc tggtcgatca gggcagcctg ctggcgctgc gcaccggctt cggcgccggc
     4921 gtgcacaccg cgctggcgcg catcgaaggg cggccggtgg gcctgatcgc caacaacccg
     4981 ctgcacctgg gtggcgccat cgacgccgcc gcggccgaca aggcggcgcg cttcatgcag
     5041 ctgtgcaacg cccacggcct gccgatcgtc agcctgatcg acacgcccgg cttcatggtc
     5101 ggcccggagg tggaacagac ggcccaggtg cgtcacgtca gccgcctgtt cgtcaccgcc
     5161 gccagcctgc gcgtgccgtt cttcagcgtg gtgctgcgca agggctacgg cctgggcgcc
     5221 atggccatgg cggcgggcgg cttccatgcg ccggtgttca cgctgtcctg gcccaccggc
     5281 gagttcggcg gcatggggct ggagggctat gtgcggctgg gttttcgcaa ggagctcgaa
     5341 gccgcggccg agggcgccga gcgcgacgcc ctgttcgccc gcctgctggc cgaacgctac
     5401 gagcacggcg ccgcgctcaa catggccacc acgctggaga tcgatgccgt catcgacccg
     5461 gccgagacgc gcgcctggct ggcgcgcggg ctggcgtcgg cgcgcctgcg cccggcgacg
     5521 gggtcgcgct tcgtcgatac ctggtagcgc agcccgcgcc cgtggcgccc ggctggcgcc
     5581 gaacgcgcca cggcggggcg tgccgccgtc gccgtggcgc tcagcgcagc gtggtgcagg
     5641 ggtcgcgcgg cggcagcgcc ggggtcggcc acagcaggtc gccgggctgc agggcggtgc
     5701 ccgccaatgc ttcgttttca ggagctgcct gcgcttgctc cgcctgcgct gaaagcactt
     5761 ttgacttcaa gctcgggggc agaaagcgcg gcacgcccag cgcgcggtgc acatggccgt
     5821 tgccggtgat cagcagcacc acgcggccgg gcgcggcctg gcgtgcggcg ccggccacgg
     5881 catcggccat ggcgatgtcg cgcgcgatct gcacccgcgt catggggtcg atctggcgct
     5941 ccggcagcag gccgcagtgg ctggcgcgga tgcgctggcg ctgcaccgcc agcagcgccg
     6001 gcggcaggcg cgcgtccagc gcgctctggc gcatggcctc gcgcatctgc gctggcgaca
     6061 ggttggcccc ctgcaccggc gcgccggccg ccaccgcgcg catgatcacc gggccgtaca
     6121 cctgccacgg ccagcgcgcg tcgtcccagc ccagcgcggc gcgcacctgg tgcgcacggg
     6181 cgtcgcgcgg caggccggcg gtgctgcgtc cgccttcggc catttccagc accactgcgg
     6241 ccaattggcc gcgatcgccc agccactgca ccacctggcg ctgcaggtgc tggtgttcgg
     6301 gcgcgtcgtg ctgttcgccc agcagcaggg cgtcggtcgg cagcagggtg gccagccttg
     6361 cctgctggcc ggcatccagc gggggcaggg cgcaggccgc cagcaccagg gtggcaagca
     6421 ggatcaggcg tcgcatgggg gcgatactaa gcggctggcc ggtcgtcatg ccttcgtctg
     6481 ccgttccccc cttcgtgcgc gctgcgctga ccctgctgct ggcctggggc gcggcgcggc
     6541 tgggcgtatg gctgtcgctg ccggtgccct ggatgctggg ccccttggtg gccaccgccc
     6601 tggccagcat ggccggcctg ccgacgcaca gcgtgcgcgc cttgcgcaac gccggccagt
     6661 gggtgatcgg ggtcagcctg gggctgtact tcacgccggc gatggcggcg ctgatgctgg
     6721 gccattggtg ggcgctgctg ctgggcgtgc tgtgggcact ggccctgggg ctgggctttg
     6781 ccgcctggct gcggcgggcc acgcgcgcca gtctgggcga gctgagcccg gcggagcgcg
     6841 ccgccacgct gcactttgcc agcaccatcg gcggcgcctc cgagatgacc ctgctggccg
     6901 agcgcgccgg cgcccgcacc gacctggtgg cggccgcgca cagcgtgcgc atggcggtgg
     6961 tggtgctgag cctgccgttt gccatgcagt gggcgcgcca gcactggcag ctgccggcgc
     7021 tggacgtgct gccggcggcc gagcgggtgg tcgacgggcc ggggctgctg tggctggcgc
     7081 tggcgaccgg ggccggcgcc gccctgctgc ggcgcaccgg cggcgccaac ccctggttca
     7141 tcggcccgct ggcggtggcg atggcgctga ccatgctgga ccgccacctg tcggccgtgc
     7201 cgccggcgct gggccaggct gcgcagctgg tgatcggggt cagtctgggg gtgcgctttt
     7261 cgcgccgctt cctgcacgcg gcgccgcgct ggctggcggc cgtggcgctg ggcacgctgg
     7321 gcatgatcgc gctgtgcgcg gccatggcct gggtgctggc ctgggccacc gggctgcacc
     7381 cggtgacgct gatgctggcc acctcgcccg gcgggctggc cgaaatggcc atcaccgccc
     7441 aggtgctgca actgggcgcg ccggtggtga cggcgtttca ggtgtgccgg ctggtggcgg
     7501 tgctgctgct ggccgagccg ctgttccggt gggggtcggc gcggcgctga cgcgaacgcg
     7561 ctcagtgcat cacgaccggg ttctgcgcca gttcggcgta gccttccagg gtttcctcga
     7621 cttcttcctg ggtgggggtg ttttgctgcc aggcggcgat gcgctgctgg aacagctcgg
     7681 cccataggcc gtcgaggtag acctccttgc ccgagcgttt gtccacgatt tcgaagccgt
     7741 ggcgcgccag cacgggctgg cccgcctgcg ccggcgtatc ggccagcacg tcgggcaaca
     7801 ggtgaacgac cgcaaagctg tcggaatcgt agagcgtgtt catcggtggt caggcccctc
     7861 ggatggcagt ggttgatgcg aaagtctcac tggcctgcat atgagaacgc atcgggcgct
     7921 ttcaaggtag acgcccgcca ggcggaaaac ccaattaagt ccggtttcga aggcctcagc
     7981 gcggcgtcgg caggctggac agttgctgat cggccacatc gccgctggcc tgggtgacgc
     8041 gcaggcgcgc cggcaggtag tgcagcgcac gcgccagcca cagctcgatg cgcgtgtcgt
     8101 actcgtgccg gggcgcgcgc tgcaggcgca cgcagggcac ggcctgcccg tcgagctgca
     8161 gcacttcgtc gtccagcact tcccagcgcc agtcggcggc ctcgcgcgcg ccggcggtct
     8221 gcagcacgat ctggctgccg ggcgggtagc gtggcgggtc ggcggcgatc aggcccccca
     8281 gctgcagcag cgtgctcagg cggtcctggg cgccggcctg cagggtggtg tccggcgtgt
     8341 tggcggaaaa gcggatgcga ccgccctcgg cgtcgaaatg cgcggcgcgc tcgccccgct
     8401 ggcgctcggc gtagcgctcg ggcaccagcc cggccggtgt gacgcggccg gtgctgtgct
     8461 gccggcgcgt gccgatgggc ggcagttgca gcgtccactc ggcctcgtag tgcgagcctt
     8521 cgcgctgcca gcgcagctgt gcgtcggcct gcagcggcag gccgcgcgcc aggccgctga
     8581 cgcggtagtg cagggtgacg gtgtcggcca gccgcaccgg cgccgccacg gcgacggccg
     8641 gtgccgcggg caccgcgggt gtctgtcgac cgtccgtctc ggtgcgcggc ggtggtgccg
     8701 ctgcgctggc gcgcgcgcgc ggcgtcaggc gccgcgggct gggcgcggtc acggccggcg
     8761 tgcgcgcagt gccggccggg gacgtggcgc ggccgggggc ggatggcgtc gcgccggcgc
     8821 tggcgacggg cgtgacggcc ggcgtggcgg cgggcgccgg gatggcgcgc agccacagcg
     8881 gcgcggcgcg gggggcaggc gcgggcgtgg cccggggcgg cgcgggccgc agcagcagga
     8941 catgcgccag cagcacggcg cccaccagcg ccagcggcgg acgcgcgcga tgcggcggcg
     9001 gctggcggct catgcactca acactagaat cgccgcttgc gggcgcgcac cgatggccgc
     9061 gcccgcgccc cgcccgccgg tccgcgccgg ccgtgcgtct gtgcggcggg gcttcgctga
     9121 cacgcatcca tgaaactggc aacctacaaa gacggctcgc gcgacgggca gctggtggtc
     9181 gtctcgcgcg acctgacgac ggcccattat gcgaccggca tcgccagccg cctgcagcag
     9241 gcgttggacg actggaactt catcgcgccc cagttgcagg acctgtacga cgcgctcaac
     9301 ggcggccgcg cgcgccaccc gttcgccttc gagcccgagc gctgcatggc gccgctgccg
     9361 cgcgcctacc agtggctgga cggctcggcc tacctcaacc atgtcgagct ggtgcgggcc
     9421 gccggccgcg ccgacgtgcc cgagcgcttc tacaccgagc cgctgatgta ccagggcggc
     9481 agcgacgatc ttctggggcc gtgcgacgac atccgggtgg cggacgaggc cttcggcatc
     9541 gacttcgagg gcgagctggc ggtcgtcacc ggtgacgtgc cgatgggcgc cacgcccgag
     9601 caggggctgg aggccgtgcg cctgctgatg ctggccaacg acgtcagcct gcgccagctg
     9661 atcgccgccg aggtcgagac ccgcctgggc ctggtgcagg gcaagccctc caccgccttc
     9721 agcccggtgg cggtgacgcc cgacgagctg ggcgaggcct ggcaggacgg tcgcgtgcac
     9781 ctgacgctgc aggtgagctg gaacggccgc aaggtcggcc tgtgcgaggc cgggccggac
     9841 atgcgctttc acttcggcca gctgatcgcc cacgccgcgc gcacccggcg cctgcgtgcc
     9901 ggcagcatcg tcggcagcgg caccgtcagc aaccccggcg tggatcaggg cggccggcgc
     9961 gactggccca agggctacgg ctgcatcgcc gaaaagcgcg ccatggaaac cctgcaggac
    10021 ggcgcgccgc gcaccgacta catgcggttc ggcgacaccg tgcgcatcga aatgaagggg
    10081 cgcgacggtc gcccgctgtt cggcgccatc gatcagaaag tggttcaaca gggctctggc
    10141 gcttgatgga caagcgccaa cagctattca aacaatagca ttcagggcgg cgccgaggcg
    10201 cggagtcagg tgcgcggccg gcgccacggg gcggcccctg tcctgcccgt gacacgcctg
    10261 gggccagtcc cgatgcgcgc ccgcttgaac tgcgctgcaa tgggccagcc tgcacgctca
    10321 ccgccgccca tgaacacgcc caccctgcac accctgcgcc tggacatcga cggcgacgcc
    10381 gtccgcgcca ccctgtgccg ccccgagcgg ctcaacgccg tcggcgcgca actgctggac
    10441 gaactggtgg tgctggccga ctggctgggc gagcgcgagg atctgcacta tctggtgctc
    10501 gatcacgagg ggccggtgtt ttcggccggc gccaacctgg aagaagcgcg cgagctgttg
    10561 gccgacgccg gccgcgcgcg ggcccgcctg cgcgccaacc agcgcgctgc gcagcaggcc
    10621 ctgatcaagc tcgccgcgct cgagcagatc agctttgcgg cgctgcgcgg ctcggcctac
    10681 ggcgccggcg tggccatcgc catggcgtgc gactttcgcg tcatggccga ggacgcggtg
    10741 ctcaacctgc ccgagacgcg gctgggcatg ttcctcagct atggcgccac gccgccgctg
    10801 gtggcggcgc tggggctggc gcgcgccaag gaaatgatcc tgttcgccga ggactgggac
    10861 gccgccaggt gcctggccgc cggcgcggtg cagcaggtgg cggcgcgcgg gcaggtgggc
    10921 gcgcgcatcg acgccatgat cgccaccctg cgccagcgcc actggccggc ggtgcgcctg
    10981 gccaagcagg tggccaacgc ggcggcgccg gcacgctggg gcgaggtcac cctgaccgaa
    11041 cccgagctgg ccgccgaagc gctggccggc ggcgacgtgg cggcgcgcgt ggaggccttt
    11101 ctgcgccgcc accaccccga gcgctgagct tgtgagcaaa acatggctct ggggcgctcc
    11161 aggtgtgcgc cagaagctat gtaaaagata gcattcgagg cggcgctcgg cgctttcagc
    11221 cgccacccag ctgcgccgcc agcgtgcgga aggcgcgcag cagcatctcc accgccaccg
    11281 ccaccaggat caggcccatc aggcgctcga aggccatcag cacgcgcggg ccgaggcgct
    11341 gctgcaggcg ctcggccagc agcagcacca gcgcgcacac cgccatcgtc acgctgagcg
    11401 cacccaccca ttcccagcgg cgctcgggcg cctggctgac cagcagcatc accgtggcca
    11461 gcgccgacgg gccggccagc gccggaatcg ccagcggcac gatcagcggc tcggtcgggg
    11521 ccggctcgtc gggcggctgc gccgacggaa acaccatgcg cagcgcgatc aggaacagca
    11581 ccacgccgcc ggccagctgc agcgccagcg gcgacaggtt catcaggcgc agaaagccgt
    11641 cgcccagaaa catgaacacc agcagcagcg caaacgcgat cagcacctcg cgcaggatca
    11701 cgcggcgccg gcgcgtcggc ggcaccgccc gcagggcgtg ggtgaagatg ggcaggttgc
    11761 ccaccgggtc ggtgatcagc agcagcaaga tggtggcgga ggcgaagctg tcgttcatgg
    11821 caacgggagg cgccgaagcg ggcgcatggg cgcgattgtg tcgcgcggcg cgaggcatga
    11881 cagatcgcag gcgccggtgg ttgcttggga agtcctcgca ttgttgcgcc gatcaatcgg
    11941 cagcgattgt ccaaccatca ctgttagcta caaaaacaca gtgatctggt cttgcgatct
    12001 gctcttggtc gagcatcggc taccatgcag cccatgtctt ttcatcacct gagcccgccg
    12061 atcgccgtcg ccccgcctcc gggtgcgccg gcgctgggct gaatccccgg cgccgatcga
    12121 tcggtttgcg gcaccgcccg gtgtgggcgg gctgccgttt tgttcgggct tttcaggaaa
    12181 ggtgatgtcc catgcatgca tggccgacgg cgctgttcgt gctgctgtgg agcagcggcg
    12241 ccattgtttc tcgctggggg ctggatcacg cctccccctt cattctgctg acgatgcgct
    12301 ttgcgctggc actgctggtg ctggccgcgc tgggctggcg cacgcgtgcc tggctgccgg
    12361 caccgggcac ggccgctcac gtggctgcgg tcgggttgct gctggtgggt ggctactcgt
    12421 gcagctactt tctggcgctg gaccaggggc tgacgccggg cgcgctggcc accatgctgg
    12481 gcgtgcagcc catcctcacc ttgctgttgc aggagcgccg cgtcacaccg atgcgcctgc
    12541 tgggcctgct gctggccctg ggcggactgg tgctggtggt caaggacagt ttgctgcgcg
    12601 cgcaggtgtc gctggcaggc atcgcgttgg cgctgcttgc gctggcctgc atcacgctgg
    12661 gtgcgatggc gcagcagcgt ttggcccagt cgcccatccg tgtgctgccg cttcaatgcg
    12721 ccgtggcgct gctgctgtgt ctggcgctgg taccgacgca gccgttgcgt ctggaagtgg
    12781 gcctgccgct gctggcctgc gtgctgtggc tgggcgcggt gatctccgtg ggcgctaccg
    12841 tgctgctgta tcgcatgctg caggccggca atctggtcaa cgtgacgagc ctgttctatc
    12901 tggtgccggg cggcacgctg ttgctggatg cgtggttgct gggccatccg gtgacgccgc
    12961 tggcgctcgg cggactcgcc ctggtcgtgg gcgggctggc gctggtgctg cgcacgcgac
    13021 cggcctgaat cggcgcttcg ctaccatggg cgggttcagg acaattcacc ggagactttc
    13081 tgcatgagcc agcccgccat cgactacagc cgctaccaga cctttcgcat cagccgccgc
    13141 ggcgagggcg gctgcgtgct cgacctgcag atgaaggccg ccaacggcaa gctgcccacc
    13201 gccgggcatg aggggcattg ggaactggcc gagatctggc gcgacatcga caaggacgac
    13261 agcgtgcgcg ccgtggtgct gcgcggcgaa ggcatgggct tttccggcgg cggcgatctg
    13321 gccctggtgc aggacatggc cagcgacttc gccgtgcgct cgcgtgtgtg gaaggaagcg
    13381 cgcgacttgg tctacaacgt catccactgc gaaaaaccca tcgtcagcgc catgcacggc
    13441 ccggcggtgg gcgccggcct ggtggccggc ctgctggccg acatctcgat cgccgcccgc
    13501 agcgcccgca tcgtcgatgg tcacacccgc ctgggcgtgg ccgcgggcga tcacgccgcg
    13561 atttgctggc cgctgttgtg cggcatggcc aaggccaagt actacctgat gctgtgcgag
    13621 gcggtgaccg gcgaagaggc cgagcgcatc ggcctggtgt cgctggcggt cgatgacgac
    13681 caactgctcg ccaaggccta cgaggtggcc gacaagctgg ccgccggcag caccaccgcc
    13741 atccgctgga ccaagtactc gctcaacaac tggtaccgcc aggccgggcc cagcttcgac
    13801 acctcgctgg cgctggaatt catgggtttt ggcgggccgg acgtgcacga aggcgtggcg
    13861 tccttgcgcg agcggcgccc gccgcgctac tgagccgcgc gccccacgaa agctattaaa
    13921 aacatagctt ccggcgcttg ctgcaagcgc gccgaaggct gattcggctt caggtcttgg
    13981 gctgcatgcg ggccatcatg agggcgatgt tggcgcggcg ctcggcctcc acgcgctgca
    14041 ccgccggccg ctggttcagg cgttgcaggt agtccttcac cggcagcgcc gccagcaggt
    14101 cctggccgta gatgatcttg ctggcgccgc tgaccagcgg caggtgcacg gcggcggcgc
    14161 agtcggccag cgtgaactgc tcgccggcga tgtaggggcc gaacttggcc agccgggcga
    14221 aggccgccac gtgcctttcc agttgcgggc ggatgcgctg cttcaggccg tcgctcacct
    14281 ggccaccgaa gaaggcctgc gggtacaggt tgcgcgccac cagctccagg tgcagctcga
    14341 tgtagcgcag cagctcgcgc accttggcgg cctggtaggg gctggcgggc agcagcggcg
    14401 tttcggggta gcgctgctcc agatattcga ggatcaccgt cgattcgctg atcggcccgt
    14461 cgtcggtgag gaggtagggc accttgccca gcgggctggc ggtggtgtcg gtctcgccca
    14521 cccaggcggt gacttcttcg aacggcacgc ccttttcgag cagggcgagc ttgaccttgt
    14581 tgtagtagtt gctggcggca aagccacaga gcttgagcat gatgagcgat ctccggtgac
    14641 gggaaaaggg cggggggcga cgcgccggcg gcagcatagc agccgcggcc cgcacttgat
    14701 gtcgcgcaat ggtctgggtg ggggcctggc cataatgcgc gtcggatgaa cccgctgcgc
    14761 cacgcccacc gccttgcccg ccaggtgctg gtgtggtttg tgctggcgct ggtggtggcg
    14821 gtggctgcgc cgctggtgcg cgcgcagccg ttcgaactgc tgtgcggtgc gggcggccag
    14881 atcaaggccg tggtgcacac cgccagcggc gacacgctgg agctgggtgc cggccacctg
    14941 gactgcccgc tgtgcgtccc gaccggtgcg cccgggccgc tgccccgtct gggcctggcc
    15001 gggccggcgc cggcgtgcgt gcccgacccg acggcgcccg ccctgcgcct ggcacccgcg
    15061 ccgctgccct ggcaggcccg cgcgccgcct ccgacccact tccgatagct actgttttga
    15121 tagctttcgg cgctgacggg gcgggcgata ggggctgatt cgatcttcag tgccagaggc
    15181 ccgggccgtt gcgccggcgg ggtaccgctg ggggcgacgc gccccacggg cccgccactg
    15241 cctgtgggtc gaatgttcag aaaggttttc tccatgaaac gtcttgccat ttccctgtcc
    15301 gccctggcgc tgtgcgccgc cgcctcggcc caggtcacca tcaccgaacc ctgggtgcgc
    15361 ggcaccgtgc cgcagcagaa ggcctcgggc ctgttcatgc gcatcgacgc cgagcagcac
    15421 gtgcgcctgc tgggcggcag ctcgccggtg gccggcgtgg tcgaagtgca cgaaatggcc
    15481 atgcagggcg aggtcatgaa gatgcgcgcg ctcagccacg gcctggagat cgccaaaggc
    15541 cacagcctgg tgctcaaacc cggcggctac cacgtcatgc tgatggagct caagcagcag
    15601 ctcaaggccg gcgaccgcgt gtccgtgacg ctgaccttcg aggacgtcgc cagcaagcag
    15661 cggttcacgc gggaagtcca ggccccggtc accgcgctgg gcggcgccca gcccgcgccc
    15721 gcagcgcacc accggcactg accccaggag gctggcgacg gtcacgcggc gcgccggaac
    15781 gtgccatatg cgttaagctg cgcagcactc ttcaacgcct gatcgggagc ggtggccatg
    15841 agcgattcca gcaacttcga cttcggcaag ttgatccctg gcttcgattt cctgcagaac
    15901 ctggccacca gcgccaaggg tgtcgccgcc ggcccggtgt cggcgctgcg caactgggtg
    15961 gcgcctaccg tcagcgtgga agaactggac aagcgcatca ccgagctgaa ggcggtgctg
    16021 ttctggctgg agcagaacag caccgcgctc aaggccacca ttcaggcgct ggaggtgcag
    16081 aagatgacgc tggccacgct ggccggcatg aacctgagca tgagcgaggt cgccaaggcc
    16141 ttcaccgtgg ggtcgcccgc caagccggac gccgcagcgg ccagcgcgcc ggctgcgcca
    16201 ccgcactggc cgttcggtca ggcgccggcc gcagcaccgg ccgccgagcc cgaacccgag
    16261 cccgagcccg agccggaacc accggcgcgt gccagggcgc cggcgcgcaa gagcaaggcc
    16321 aaggccaccc cggccgccac tgcggccgcc ccggcgccgg ccctggccga tccgatgcag
    16381 tggtggggtg cgctgacgca gcagttccag cagattgccg ccaacgccgt gcgcgaggcg
    16441 gcgcgtgcgg tgccggaaca gcccgccgcg gcgccggcgg ccaccgcggc ggccgccacc
    16501 gccaagaagc cggccgccag gaaggcgccg gcggcccgca aagcggcgcg caagccggcg
    16561 gccaagaaag cggcctcgcg cgccaaggcg gcgaccgaag ctgcacctcc ggccaccgag
    16621 acccgcggcg ccaccctggg ctggccgctg ccgccgccgt tcaagctcgg tcgttgaacc
    16681 tagaaacggc agttgaccgc tggtcaactt cgggcagacc gcatcgatgc gggggctcgc
    16741 ggctgcgatg gcct
//
LOCUS       DAOQTZ010000064        16377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4032_length_16377_cov_13.598517, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000064 DAOQTZ010000000
VERSION     DAOQTZ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16377
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4032_length_16377_cov_13.598517"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKN51_08790"
     CDS             <1..228
                     /locus_tag="PKN51_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNT85235.1"
                     /translation="RQKGHTELYRGAEYVVDFLPKVMIEVVVTDADVDRCVEAIVKSA
                     RTGKIGDGKIFVTPVERVVRIRTGEEDDAAV"
     gene            320..775
                     /locus_tag="PKN51_08795"
     CDS             320..775
                     /locus_tag="PKN51_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85236.1"
                     /translation="MTRKTAGTLVFGILSVLGWLLTAYLARGAGASARASMFAWAAGF
                     VALILTLHTLLYALLADRKARDADWLRRHGRAVQAEVVKIGRRGKGWRVQALAVAPTG
                     TAAPTVFKSDILRADPRPRLAVGDHIQVHLHPTDRRRYWMAIGTESKYL"
     gene            882..1121
                     /locus_tag="PKN51_08800"
     CDS             882..1121
                     /locus_tag="PKN51_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85237.1"
                     /translation="MTMHVWVIAATLWVSLGVPAQAQPRVEYLTEDPPKGSLPHRKVV
                     YVDDRSCPKGEVKEVTGGSERQSIPRKVRCVKRPG"
     gene            1498..3198
                     /locus_tag="PKN51_08805"
     CDS             1498..3198
                     /locus_tag="PKN51_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNT85238.1"
                     /translation="MRPHHAFWPKRLPYSLTPPATSLWQNLAINALRYPDKPATVFFG
                     TVLSWSQLKLSAERLAARLQALGVAKGDRVLLMLQNTPQLIVAHFAILRADAVVVPIN
                     PMNKAEELKHYITDPDARVAITSADLAGELAAASDALPAAQRLAHLIVTQYSDAFDAG
                     VQGDDAPPEHWRDWLLTARALPQLAGGQVHTWRDALAFGGPLAETTVGPQDLSVLPYT
                     SGTTGLPKGCMHTHGSIMHNAVASSLWGTGHSESVVLVVVPMFHITGMVSVMHSAVYS
                     AATMVLMPRWDRELAGRLISRWQVTGWTNIPTMIIDLLASPNFASFDLSSLRTIGGGG
                     AAMPAAVAQRLEEQYGLKYAEGYGLTETAAPSHSNPPDRPKQQCLGIPFMSTDARVVD
                     PETLKELPQGETGEIVVCGPEVFAGYWKRPEATAAVFFELDGKRFFRTGDLGRVDEEG
                     YFFITDRLKRMINASGFKVWPAEVEALMFKHPAIQEACVIGFKDAYRGESVKAVVVLR
                     ASHKGQVSEQDIVEWCHANMAAYKAPRAVQFVDALPKSGTGKVMWRTLQEAEASVSVH
                     "
     gene            complement(3356..9343)
                     /locus_tag="PKN51_08810"
     CDS             complement(3356..9343)
                     /locus_tag="PKN51_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNT85239.1"
                     /translation="MHRSPALDRALRPTLLVLCGLAASVGAHALGIRSLSPQGEVARV
                     NQVVASFDQDAVRMGDAGAPAPLRVDCDQAAAARGQGRWRSAREWVFQFEADLPPGVR
                     CRVTPEPGFQSPTGAPWTGARSYSFHTGGPFVQQLVPAPTSTIEEDQAFVLRLNGEAS
                     ADSLNAHLWCAVDGLGERVPVRLVEGAPRAALLKHLGWDKAAQQTPGRYVALQCNRRL
                     SAAARVQLVFGAGVATPSGVLNRVERRFAFTVREPFSASLSCERENAQAACMPIRPLT
                     LKFSAPVTRAQAAQARLRADKLEYEPVFDEPQDGDALIEQLRFPAPLPERRSFTLHLP
                     ADLKDASGRALTNASSFPLRVSTGPLPPLAKFAAAPFGVIERFAEGPDGPALLPLTLR
                     RVEPALQAQALHIHSLQPQSDADIIAWFKRVQRYDEAYVPRALARRDLGERLPPPVNE
                     QTRDQVESRTLSLLQGRSGLNTLTLPAAPKEGERPFEVVGVPLPPGFHVLELASQQLG
                     EALLDARYGARRPMFVRSSTLVTNLAVHFKLGRAGSLAWVTALDSGKPVADALVRVSD
                     CHGKPAAEARTDAQGIARFDRLSPEPPSCSGEDEFYADFQQAWFVSARARQAGVDELA
                     FTWSSWQRGIEPWRFNVPTSSQPQPDLRAHTVFDRALLRTGETVSMKHLLRSETPGGF
                     GLPPQMPATLVITHLGSGQTFTQPLQWRRTATGGRSAESSFAIPQAAKLGVYQVTLRH
                     GGAAGADGGDDAGDIETGSFRVEEFRLPVLQGRVAPQSGEPLVAATQVPVQVQVSYVS
                     GGPAARLPVRVSALTRGKTLSFADYEAFSFEPPRPPAAGPDRSDDAEPQDAARQDQQV
                     VADKLAVTLDAQGSGQTRIEAIAAAPQARELVLEASYADPNGEIQTLRGSTTLWPAGV
                     IAGIRADSWTSVQQTLKMQALALDLDGQPRAGVPLDVKAVARSTTSSRKRMVGGFYTY
                     DNRTETHELGTLCTGRSDTRGLLTCDIRLTHPGEVELVVSARDPQGRASQAARSVWVT
                     RQGELWFGGEDHDRIDVLPEKREYAVGDTARFQVRMPFRRATALVAVEREGVMQTQVV
                     ELRGDDPTLTLKVGPDWSPNVYVSVLALRGRLVEVPWYSFFTWGYRRPRDWWQAFWHE
                     RGDYAPPTALVDLSKPAFRLGMAEIRVGTDAHRIAVDVQADQTSYPVRGKARVRIQAR
                     LPGGQPAAHAEVAVAAVDEALLELLPNRSWDVLPAMLRRRAWGVQTATAQMEIVGRRH
                     YGRKAVPAGGDGGTAPTRELLDTLLLWQPRVVLDDQGRADIEVPLNDALSTFRVVAVA
                     DSGTALFGSGHTRLRVTQDLQIIGGLPPLVRGGDRYRALLTLRNTTTRPMQVQVSPRA
                     TLVELKPQSVDIPAGEAREVGWDVTAPPELATLRSGEILWEVEARDRASGARDALKLS
                     QRVLPAVPVTVQQATLTQLEGPLTLQLAAPAAALPGRGGVGLALQPTLADGLPGVRDW
                     LARYPYACLEQQTSVALGLRDATRWQKTAAQLPVYLDADGLAHYFPPRAGDEHRGSDT
                     LTAWLLAATHEASRLDAAFALPAALRAQMVAGLTQFVEGRIERRHWSPRGDLDVRKLA
                     ALEALSRHGAVRPAMVSSLTIAPQQWPTSAVIDWINLLRRVPGIADQRTRLQEAQQVL
                     RARLSFQGTRLAFSTEQDDHWWWLMAGGDVNAARLLLSALDDPAWQADLGRLASGLIA
                     RQQSGAWTTTTANLWGSLALEQLARQREREAVAGTTQAALGDARDKVDWSKVVRLKAD
                     EPGSAAHARSLFGAPASPGQWTGNRLLLPWPEPPGAPAPLTVTHQGSGKPWLTLQALA
                     AVPLAAPVADGYQITRSVHAVEQANPALPAGQYSRGDVLRVTLDVQARADMTWVAITD
                     PVPAGATVLGSGLGRDSDLATQGERRSGQGWLAYEERSFEAFRAYYEFLPKGKLSLSY
                     TVRLNNPGRFQLPPSRVEALYAPEMHGLTPNAPLIVH"
     gene            9521..10726
                     /locus_tag="PKN51_08815"
     CDS             9521..10726
                     /locus_tag="PKN51_08815"
                     /inference="COORDINATES: protein motif:HMM:NF020322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT85240.1"
                     /translation="MESPVGLLSQPALQAALLAAAGALAYWLKDLPRLLLQWGRRFFV
                     STLTVDSRDEFLFPALVEHMDQHPGLAGVNQFTARSVRRGTEHQSLAEDLRAGQPPRA
                     FLSPGEGVHILRVDGRWLWLRRELQVGQSVFERLSLSTLGRSPRYLQDLLQAAIDARA
                     ARETDRLSVYLPNPFHGGDWMRARLGWRRPLASVVLKAGQAEALLADLRRFLASRERY
                     AELGIPWRRGYLLYGPPGTGKTSLVTALASELHLNVCTLSLASPVVTDEKIHTLLAAV
                     PQRALLLIEDVDAFFREREAAHAQVRLSFSGFLNALDGVATQEGTLLFMTTNHLERLD
                     PALIRAGRIDEQVELGHADEDQLRRLYLKFHPDPAAAAVFARERAGQALSPAAVQGEL
                     MKRFGTDPV"
     gene            complement(10760..11575)
                     /locus_tag="PKN51_08820"
     CDS             complement(10760..11575)
                     /locus_tag="PKN51_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNT85241.1"
                     /translation="MDLRPLALAALCEADVERKLALVAALQAPAATLYIAPETALEAP
                     PGLPGRPLRPPLIDPARVPQRSVHTREGRAALVHAICHIEFNAINLALDAIWRFAGMP
                     EAYYRDWLRVAIEESHHFSLLHAHLQTHHQLRYGDFEAHDGLWAMCEKTRDDLTARMA
                     LVPRTLEARGLDATPLIQRKLRAVATPDALAACDILDLILREEIGHVAIGNHWYRWLC
                     ARQGLEPLAHYRRLTRQHRAPRLRGPFNTEARRQAGFSDDELRALSADPPTPS"
     gene            11623..12147
                     /locus_tag="PKN51_08825"
     CDS             11623..12147
                     /locus_tag="PKN51_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNT85242.1"
                     /translation="MAVYELDGVAPQVDPAAWIADSAEVIGNVSLGAGASVWFGCVLR
                     GDTERMMIGEGSNIQDLSVLHADVGQPLTVGRHVTVGHKVMLHGCTVGDESLIGIGAV
                     VLNGARIGKNCLVGAGALVTEGKEFPDGSMIVGAPARVVRQLTPEQIEGLRQSARHYV
                     DNARRFQAGLKRLG"
     gene            12165..13151
                     /locus_tag="PKN51_08830"
     CDS             12165..13151
                     /locus_tag="PKN51_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNT85243.1"
                     /translation="MSELHKFIFDGLPVRGQLVRLTDAWQEVLRRRAANTQTGAWPPV
                     VAEMLGEMAAATVLMQGNIKFDGALIMQIFGDGPLKLAVAEVQPDLRLRATASVVQSV
                     TPGMALPELVNRHGRGRCAITLDPRRRREGQQAYQGVVPLVDEGGAPLQTLASVLEHY
                     MRQSEQLDTTLVLAADEQVAAGLLIQRLPVQGQDNLSQSSVAREDAGSAADDDYQRIR
                     LLAHSLQRAELLSLDADTLLHRLFWQEPLLRFAPLAGHQGPRFACSCSRERVAAMLQG
                     LGQPEAESILAERGEIEVGCDFCGQQQRFDAVDVARLFTPAAQQSPQPGSVQ"
     gene            13172..13936
                     /gene="trmD"
                     /locus_tag="PKN51_08835"
     CDS             13172..13936
                     /gene="trmD"
                     /locus_tag="PKN51_08835"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNT85244.1"
                     /translation="MRFDVITLFPELFAPFLEAGVARRAYASGQIVLKLWNPRAYAEG
                     NYRRVDDRPFGGGPGMVMLAEPLARCLAAVQAERAGGAAPVVLFSPIGQTLTHAGVER
                     WAAGAGAVLICGRYEGIDQRFIDRHVTHQISLGDFVLSGGEIAALALLDAVARLQPGV
                     LGDEGSHHQDSFNLAVDGLLDCPHYTRPEVWDGVPVPSELLSGHHARIEGWRREQRLR
                     LTARHRPDLIVAARAAGRLSAHDEVLLAQLGRGGPG"
     gene            14077..14460
                     /gene="rplS"
                     /locus_tag="PKN51_08840"
     CDS             14077..14460
                     /gene="rplS"
                     /locus_tag="PKN51_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNT85245.1"
                     /translation="MNLIQQLEKEEIERLGKSIPEFAPGDTVIVSVNVVEGNRKRVQA
                     YEGVVIAKRNRGLNSGFTVRKISNGEGVERTFQTYSPLIAKIEVKRRGDVRRAKLYYL
                     RGLSGKKARIKEKLPGRAASPTAAD"
     gene            14562..15299
                     /locus_tag="PKN51_08845"
     CDS             14562..15299
                     /locus_tag="PKN51_08845"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNT85246.1"
                     /translation="MSTPTPLSKLPNFDPRQVPVTGIDAHLPPVPAEALTLPALRRRF
                     QAPPPWQPELRQEPRFTDRVPAAAAVLVPLVDRPQGLSVLLTERSGNLSTHSGQVAFP
                     GGKVDPEDADAVAAALREAEEEVDLSRRFVDVLGQLPVYVTGTQFIVTPVVAVVQPGF
                     VLRPNPREVAHAFEVPLAWLMNPAHHRRHRLTWEGQAREWFSMPYVEPLSVVAGSGES
                     LPGAAVERFIWGATAGMLRNFYRFLSA"
     gene            15346..16329
                     /locus_tag="PKN51_08850"
     CDS             15346..16329
                     /locus_tag="PKN51_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HNT85247.1"
                     /translation="MAFFAIALALLLEQVRPLTSDHPAAVGLHRWVRLVGRNVDAGAA
                     QHGWLAWVLAVAVPALAVSAVHLLLSWLGGWPLALVWSVAMLYLTLGFRQFSHHFTGI
                     RDALEAGDEERARGLLAHWQHVDASTVPRSEIVRHVIEVSVLAAHRHVFGVLAWFCIG
                     ALLGLGPAGAVFFRSAEYVARYWRRKAQAADQPSSPALCLAAEQAWQLINWLPARTTA
                     LGFAIVGSFEDAIEVWRSHASRFADRNDGVILAATAGALGVRLGGASLRPLTPEGAAA
                     VPVSAAGVSGEGLPGEPPRTAHFTQVVGLVWRTVALWLLLLVLLTLAHVLG"
BASE COUNT         2257 a         5640 c         6026 g         2454 t
ORIGIN      
        1 cgccagaagg gccataccga gctgtaccgt ggcgccgaat acgtggtgga cttcctgccc
       61 aaggtgatga tcgaggtggt ggtgaccgat gccgacgtgg atcgctgcgt cgaggccatc
      121 gtcaagtccg cccgcaccgg caagatcggt gacggcaaga tcttcgtcac cccggtggaa
      181 cgggtggtgc gcatccgcac cggcgaagaa gacgacgctg ccgtctgacc gacgccatgg
      241 gtagctggcc actgcctcgg cgcggcgcgt ggggcgtcgc ccgcttctga ccgggcctgt
      301 gcgtgcaccg ggcgctgcca tgactcgaaa aactgccggc accctggtgt tcggcatcct
      361 gagtgtcctg gggtggctgc tgaccgccta tctggcccgt ggtgcgggcg cgtcggccag
      421 agcgtcgatg ttcgcgtggg ctgccggttt cgttgccctg atactgacgc tgcatacgct
      481 gttgtatgcg ttgctggccg accgcaaggc gcgcgacgcc gactggttgc ggcggcacgg
      541 ccgggcggta caggccgagg tggtcaagat cggccgccgc ggcaagggct ggcgcgtgca
      601 ggcgctcgcc gtggcgccca ccggcaccgc cgcccccacc gtgttcaaga gcgacatcct
      661 gcgtgccgat ccgcgccccc ggctggcggt cggagatcac atccaggtcc atctgcatcc
      721 gaccgatcga cgccggtact ggatggccat cgggaccgaa agcaagtatc tttgatcagg
      781 gcctggggtg cgcccggagt tcggccggtc ttcaatgacg ggcgcacgat ggtgactggc
      841 cgggtcgcgt tcgccatgcg tggcccgacg gaggacgaga gatgacgatg catgtctggg
      901 tgattgccgc aacgctgtgg gtttcgctgg gggtgcccgc ccaggcgcag ccgcgagtgg
      961 agtacctgac ggaggaccct ccaaagggca gcctgccgca tcggaaggtg gtttacgtgg
     1021 acgatcgcag ctgtcccaag ggcgaggtga aggaggtcac cggcggcagt gaacgccagt
     1081 cgattccgcg caaggtgcgt tgcgtcaagc ggcccggttg agcggccgcc tcggcgcggg
     1141 tgggtgcgta gcgctctcac ccagcaggtg aaggtcatcg cagccgcgag accctgcacg
     1201 gatgcggcct gcccgaagtt gacaagcggt caacggccgt ttctaggttc agtgccggga
     1261 cccggcgacg aatgcgcctt gcaggccgtc cgggacgggg ccttgcagca cgggcacgct
     1321 ggctcgggtt cggcggaccc gcaacggcgc accgcgtgcc cgcgcacgga cgatggcagc
     1381 cgaacggcga aggtcgcccg ggagacagcc ggctacgcag acgtcgatgt ttgatgtatg
     1441 ttgcgcccag ttcaaccttg actgacctcg tcgcgaggcc acacaggaga ccgatccgtg
     1501 cgcccccatc atgctttctg gcccaagcgc ctgccctaca gcctgacccc gccagccacc
     1561 tcgttgtggc agaacctggc catcaatgcg ctgcgctacc ctgacaagcc cgccaccgtg
     1621 ttcttcggca cggtgctcag ctggtcgcag ctgaagctct ctgccgagcg cctggccgcg
     1681 cgcctgcagg cgctgggcgt ggccaagggc gatcgcgtgc tgctgatgct gcaaaacacg
     1741 ccgcagctga tcgtggcgca ctttgccatt ctgcgcgccg acgcggtggt ggtgccgatc
     1801 aacccgatga acaaggccga ggagctcaag cactacatca ccgatcccga cgcccgggtg
     1861 gccatcacca gcgccgatct ggcgggcgag ctggcggccg cctccgacgc gctgcccgca
     1921 gcccagcggc tggcgcacct gatcgtgacg cagtacagcg acgctttcga cgccggcgtg
     1981 cagggcgacg acgccccgcc cgagcactgg cgcgactggc tgctgaccgc gcgcgcgctg
     2041 ccccagctcg ccggcggcca ggtgcacacc tggcgcgacg cgctggcctt cggtggcccg
     2101 ctggccgaaa ccacggtcgg cccgcaggat ttgtcggtgc tgccctacac cagcggcacc
     2161 accggcctgc ccaagggctg catgcacacg catggctcga tcatgcacaa cgcggtggcc
     2221 tccagcctgt ggggcacggg gcattcggaa tcggtggtgc tggtggtggt ccccatgttc
     2281 cacatcaccg gcatggtgtc ggtgatgcac tcggccgtct acagcgcagc caccatggtg
     2341 ctgatgccgc gctgggaccg cgaactggcc gggcgcctga tttcgcgctg gcaggtgacg
     2401 ggctggacga acattccgac catgatcatc gacctgctgg ccagccccaa cttcgccagc
     2461 ttcgacctgt ccagcctgcg caccatcggc ggcggcggcg ccgccatgcc ggcggcggtg
     2521 gcccagcggc tggaagagca gtacggcctg aagtacgccg aaggctatgg cctgaccgaa
     2581 accgccgcgc cctcgcacag caacccgccc gaccggccca agcagcaatg cctgggcatc
     2641 ccgttcatga gcaccgacgc gcgcgtggtc gaccccgaga cgctcaagga gctgccgcag
     2701 ggcgagaccg gcgagatcgt ggtctgcggc cccgaggtgt tcgccggcta ctggaagcgc
     2761 cccgaggcca cggcggcggt gttcttcgag ctggacggca agcgcttttt ccgcaccggc
     2821 gacctgggtc gcgtcgacga ggagggctac ttcttcatca ccgatcgcct gaagcgcatg
     2881 atcaacgcca gcggcttcaa ggtctggccg gccgaggtcg aggcgctgat gttcaagcac
     2941 ccggcgatcc aggaagcctg cgtgatcggc ttcaaggacg cctaccgggg cgagtcggtc
     3001 aaggcggtgg tggtgctgcg cgccagccac aaggggcagg tcagcgagca ggacatcgtc
     3061 gagtggtgcc acgccaacat ggccgcctac aaggcgccgc gcgcggtgca gttcgtcgac
     3121 gccctgccca agagcggcac cggcaaggtg atgtggcgca ccctgcagga agccgaggcc
     3181 agcgtctcgg ttcactgaac ctattgaacc cggaaacggc agttgaccgc ctgtcaactt
     3241 cgggcagccg gagcaatggg ttgaaccctg gcaaaagatg tcgctagcgc ttatccggca
     3301 agcgccagaa gctatcttaa agatagcatt ctgaggccct cgattcaccc tgggctcagt
     3361 gcacgatgag cggtgcgttg ggcgtcaggc cgtgcatttc gggggcgtac agcgcctcca
     3421 cccggctggg cggcaactgg aaccgaccgg ggttgttcag acgcacggtg tagctcaggc
     3481 tgagcttgcc cttgggcaga aactcgtagt aggcgcggaa ggcctcgaag ctgcgttcct
     3541 cataggccag ccagccctgg ccgctgcgcc gctcaccctg ggtggcaagg tcggaatcgc
     3601 gccccaggcc actgcccagg acggtggcgc cggccggcac cgggtcggtg atggcgaccc
     3661 aggtcatgtc ggcgcgcgcc tgcacgtcca gcgtcacgcg cagcacgtcg ccgcgcgagt
     3721 actggcccgc cggcagcgca gggttggcct gctcgacggc atgcacgctg cgcgtgatct
     3781 ggtagccatc ggccaccggg gcggcgagcg gcaccgcggc cagcgcttgc agcgtcagcc
     3841 agggcttgcc gctgccctgg tgcgtcacgg tcagcggcgc cggggcgccg ggcggttcgg
     3901 gccagggcag cagcaggcgg ttgccggtcc actggccggg gctggcgggg gcaccgaaca
     3961 ggctgcgcgc gtgcgcggcg ctgcccggct cgtcggcctt caggcgcacc accttggacc
     4021 agtcgacctt gtcccgcgcg tcgcccaggg cggcctgggt ggtgccggcc acggcctcgc
     4081 gttcgcgctg gcgagccagt tgctccagcg ccaggctgcc ccacaggttg gcggtggtgg
     4141 tggtccaggc gccgctctgc tggcgcgcga tcaggccgct ggccaggcgg cccagatcgg
     4201 cctgccaggc cgggtcgtcc agcgcgctca gcagcaggcg cgcggcgttg acgtcgccgc
     4261 ctgccatcag ccaccaccag tggtcgtcct gctcggtgct gaacgccagg cgcgtgccct
     4321 ggaaggacag gcgcgcgcgc agcacctgct gggcctcctg cagccgcgtg cgctggtcgg
     4381 cgatgccggg cacgcgccgc agcaggttga tccagtcgat cacggcgctg gtgggccatt
     4441 gctggggcgc gatggtcagg ctgctgacca tggccgggcg cacggcgcca tggcgcgaca
     4501 gcgcctccag tgcggccagc ttgcgcacgt ccaggtcgcc gcgtggcgac cagtgccggc
     4561 gctcgatgcg gccttcgacg aactgggtca ggccggccac catctgcgcg cgcaacgcgg
     4621 ccggcagggc aaaggccgcg tccaggcgcg acgcctcgtg cgtggccgcc agcagccagg
     4681 cggtgagggt gtcgctgccg cggtgctcgt caccggcgcg cggcgggaag taatgcgcca
     4741 ggccgtcggc gtccaggtag accggcagct gggcggcggt cttctgccag cgcgtcgcat
     4801 cgcgcaggcc gagggcgacg ctggtctgct gctccaggca ggcgtaggga tagcgcgcca
     4861 gccagtcgcg caccccgggc aggccatcgg ccagcgtcgg ctgcagcgcc agcccgacgc
     4921 caccgcgccc gggcagggcc gcggcgggcg cggccagctg cagcgtcagc gggccttcga
     4981 gctgcgtcag cgtggcctgc tgcaccgtca cgggcaccgc cggcagcacg cgctggctga
     5041 gcttgagcgc gtcgcgcgcg ccgctcgcgc ggtcacgggc ctcgacttcc cacaggatct
     5101 cgccgctgcg cagcgtcgcc agctcgggcg gggcggtgac gtcccagccc acctcgcgcg
     5161 cctcgccggc cgggatgtcg acgctctgcg gcttcagctc gaccagggtg gcgcgcgggc
     5221 tgacctgcac ctgcatcggc cgggtggtgg tgttgcgcag cgtcagcagg gcacggtacc
     5281 ggtcgccgcc gcgcaccagc ggcggcaggc cgccgatgat ctgcaggtcc tgggtcacgc
     5341 gcagacgggt gtggccgctg ccgaacaggg ccgtgccgct gtcggccacg gccaccacgc
     5401 ggaaggtgct cagggcgtcg ttcagcggta cctcgatgtc ggcgcggccc tggtcgtcga
     5461 gcaccacgcg cggctgccac agcagcagcg tgtccagcag ctcgcgcgtg ggcgcggtgc
     5521 cgccatcgcc gccggcgggc accgccttgc gaccgtagtg gcggcggccg acgatctcca
     5581 tctgcgcggt ggcggtctgc acgccccagg cgcggcgccg cagcatggcc ggcagcacgt
     5641 cccagctgcg gttgggcagc agctccagca gcgcctcgtc caccgccgcc accgccacct
     5701 cggcgtgggc ggccggctga ccgcccggca ggcgcgcctg gatgcgcacg cgcgccttgc
     5761 cgcgcaccgg gtagctggtc tggtcggcct ggacgtcgac ggcgatgcgg tgggcgtcgg
     5821 tgccgacgcg gatctcggcc atgcccagac ggaaggcggg cttgctcagg tccaccagcg
     5881 cggtgggcgg ggcgtagtcg ccgcgctcgt gccagaacgc ctgccaccag tcgcgcgggc
     5941 gccggtagcc ccaggtgaag aagctgtacc agggcacctc gaccagccgc ccgcgcagcg
     6001 ccagcacgct gacgtagacg ttgggcgacc agtccggccc gaccttcagc gtcagcgtcg
     6061 ggtcgtcgcc gcgaagctcc accacctggg tctgcatcac gccctcgcgc tccaccgcca
     6121 ccagggcggt ggcgcgccga aacggcatgc gcacctgaaa gcgcgcggtg tcgccgaccg
     6181 cgtactcgcg cttttcgggc agcacgtcga tgcggtcgtg gtcctcgccg ccgaaccaca
     6241 gctcgccctg gcgcgtcacc cacacgctgc gcgcggcctg gctggcgcgg ccctgggggt
     6301 cgcgcgcgct gaccaccagc tcgacctcgc ccgggtgcgt cagccggatg tcgcaggtca
     6361 gcaggccgcg cgtgtcgctg cggccggtgc agagcgtgcc cagctcgtgc gtttcggtgc
     6421 ggttgtcgta ggtgtagaag ccgcccacca tgcgcttgcg gctgctggtg gtgctgcgcg
     6481 ccacggcctt gacgtccagc ggcacgccgg cgcgtggctg gccgtccagg tcgagcgcca
     6541 gcgcctgcat cttgagcgtc tgctgcaccg aggtccagct gtcggcacgg atgccggcga
     6601 tcacgccggc cggccacagc gtggtgctgc cgcgcagggt ctggatttcg ccgttggggt
     6661 cggcgtagct ggcttccagc accagctcgc gtgcctgcgg cgcggcggcg atggcttcga
     6721 tgcgggtctg gccgctgccc tgcgcgtcga gcgtgacggc cagcttgtcg gctaccacct
     6781 gctggtcctg gcgtgccgcg tcctgcggtt cggcgtcgtc gctgcggtcg ggcccggcgg
     6841 cgggcgggcg cggcggctcg aagctgaagg cctcgtagtc ggcaaaggac agcgtcttgc
     6901 cgcgcgtcag cgccgacacc cgcaccggca gccgcgccgc cggcccgccg gagacgtagc
     6961 tgacctgcac ctgcaccggc acctgggtgg cggccaccag cggctcgccc gactgcgggg
     7021 cgacgcggcc ctgcagcacc ggcaggcgaa actcctcgac gcgaaagctc ccggtttcga
     7081 tgtcgccggc gtcgtcgccg ccgtccgcac ccgctgcgcc gccgtggcgc agcgtcacct
     7141 gatagacgcc cagtttggcc gcctgcggga tggcgaagct gctttcggcg ctgcgcccgc
     7201 cggtggcggt gcggcgccac tgcagcggct gcgtgaacgt ttgtccactg cccaggtggg
     7261 tgatgaccag cgtggccggc atctgcggcg gcaggccgaa gccgccaggc gtctcgctgc
     7321 gcagcaggtg cttcatcgac accgtttcac cggtgcgcag cagcgcgcgg tcgaacaccg
     7381 tgtgggcgcg caggtcgggc tgcggctggc tgctggtggg tacgttgaag cgccacggtt
     7441 cgatgccgcg ctgccaggac gaccaggtga aggccagctc gtcgacaccg gcctgccggg
     7501 cacgcgcgct gacgaaccag gcctgctgaa agtcggcgta gaactcgtcc tcgcccgagc
     7561 agctgggcgg ctcgggcgac aggcgatcga agcgggcgat gccttgggcg tcggtgcgcg
     7621 cctccgccgc tggcttgccg tggcaatcgg acacgcgcac cagggcgtcg gccaccggct
     7681 tgccgctgtc cagcgccgtc acccaggcca gcgagccggc gcgcccgagc ttgaaatgca
     7741 ccgccaggtt ggtcaccagg gtcgagctgc gcacgaacat cgggcgccgg gcaccgtacc
     7801 tggcgtccag cagcgcttcg cccagctgct gcgaggccag ttccagcaca tgaaagccgg
     7861 gtggcagcgg cacgccaacc acctcgaacg ggcgctcgcc ttccttgggc gcggccggca
     7921 gcgtcaaggt gttgagcccg ctgcggccct gcagcagcga cagcgtgcgc gactcgacct
     7981 gatcgcgtgt ctgctcgttg accggcggcg gcagccgttc acccaggtcg cgccgggcca
     8041 gcgcgcgcgg cacgtaggcc tcgtcgtagc gctgcacgcg cttgaaccag gcaatgatgt
     8101 cggcgtcgct ttgcggttgc aggctgtgga tgtgcagggc ctgcgcctgc aacgccggtt
     8161 ccacgcggcg cagcgtcagc ggcagcaggg ccgggccatc ggggccttcg gcaaagcgtt
     8221 cgatgacgcc gaacggcgcg gcggcgaact tggccagcgg gggcagcggg ccggtggaca
     8281 cccgcagcgg gaagctgctg gcattggtca gggcgcggcc gctggcgtcc ttcagatcgg
     8341 ccggcaggtg cagggtgaaa ctgcggcgct cgggcagcgg ggcgggaaag cgcagttgct
     8401 cgatcagcgc gtcgccgtcc tgcggctcgt caaacaccgg ctcgtactcc agcttgtcgg
     8461 cgcgcagccg cgcctgtgca gcctgggcgc gcgtgaccgg ggcgctgaac ttcagcgtca
     8521 gcggacggat cggcatgcag gcggcctggg cgttttcgcg ctcgcagctg aggctggcgc
     8581 tgaacggttc gcgcacggtg aaggcaaagc gccgctcgac gcggttgagc acgccgctgg
     8641 gcgtggccac gccggcgccg aacaccagct gcacgcgcgc ggcggcgctc aggcggcggt
     8701 tgcactgcag cgcgacgtag cggcccgggg tctgctgcgc cgccttgtcc cagcccaagt
     8761 gcttgagcag cgcggcgcgc ggcgcgcctt cgaccagccg caccggcacg cgctcgccca
     8821 ggccgtcgac ggcgcaccac aggtgggcgt tcaggctgtc ggcgctggct tcgccattga
     8881 gccgcagcac gaaggcctgg tcttcctcga tggtcgacgt cggggccggc accagctgtt
     8941 gcacgaacgg cccgccagtg tggaatgaat agctccgggc ccccgtccat ggggcgccgg
     9001 tgggcgactg gaacccgggc tccggggtga cgcggcagcg cacgccgggc ggcaggtcgg
     9061 cctcgaactg aaacacccac tcgcgcgcgc tgcgccagcg cccctggccg cgcgcggcgg
     9121 cggcctggtc gcagtccacc cgcagcggcg ccggcgcgcc ggcatcgccc atgcgcaccg
     9181 cgtcctggtc gaaactggcc accacctggt tcacgcgcgc cacctcgccc tgcgggctga
     9241 gcgagcggat gccgagggcg tgcgcgccga cgctggcggc caggccgcac aggacgagca
     9301 gggtggggcg cagggcgcgg tccagggcgg gtgagcggtg catggcggac gtcggggtgg
     9361 gcaatggatg gcagcgtagc aaaagcctgc cgtgcgtggg tggtggactg gcgcgggcgg
     9421 cgtcggggcg ggccgggcgg actcctaaag cgatagctgc tggcgcccga tcggcaagct
     9481 ctggggtacg atcgggcttt catctgatgc gagcgcgccc atggaaagcc cggtcggcct
     9541 gctgtcccaa cccgcgctgc aagcggcgct gctggctgcg gccggcgcgc tggcgtactg
     9601 gctcaaggac ctgccgcggc tgctgctgca atggggccgg cgcttcttcg tcagcaccct
     9661 gacggtggat tcgcgcgacg agttcctgtt tccggcgctg gtcgagcaca tggatcagca
     9721 cccgggcctg gcgggcgtca accagttcac cgcgcgcagc gtgcgccgcg gcaccgagca
     9781 ccagagcctg gccgaggacc tgcgcgccgg tcagccgccg cgcgcctttc tgagcccggg
     9841 cgaaggcgtg cacatcctgc gcgtggacgg ccgctggctg tggctgcggc gcgaactgca
     9901 ggtgggccag agcgtgttcg agcgcctcag cctgtcgacg ctggggcgtt cgccgcgcta
     9961 cctgcaggac ctgctgcaag ccgccatcga cgcccgcgcg gcgcgcgaga ccgaccggct
    10021 gtcggtctac ctgcccaacc cctttcacgg tggcgactgg atgcgcgcgc gactgggctg
    10081 gcgccggccg ctggcctcgg tggtgctgaa ggccgggcag gccgaggcgc tgctggccga
    10141 cctgcggcgc tttctggcca gccgcgagcg ctatgccgag ctgggcattc cgtggcggcg
    10201 cggctacctg ctgtacgggc cgccgggcac cggcaagacc tcgctggtga cggcgctggc
    10261 cagcgagctg cacctgaacg tgtgcacgct cagcctggcc tcgcccgtcg tcaccgacga
    10321 gaagatccac actctgctgg cggcggtgcc gcagcgcgcg ctgctgctga tcgaggacgt
    10381 ggacgcgttc tttcgcgaac gcgaggccgc gcacgcgcag gtgcgactgt cgttcagcgg
    10441 ctttctgaac gcgctggacg gtgtggccac gcaggagggc acgttgctgt tcatgaccac
    10501 caaccacctg gagcggctgg acccggcgct gatccgcgcc ggccgcatcg acgagcaggt
    10561 cgaactgggc cacgcggacg aggaccagct gcggcgcctg tacctgaagt tccaccccga
    10621 cccggcggcg gcggccgtgt tcgcgcgcga gcgggccggg caggcgttgt cgccggcggc
    10681 ggtgcagggc gagctgatga agcgcttcgg caccgatccg gtctgacggc aggcgcgcca
    10741 gggctggttt ggcgccgcct cagctggggg ttggcgggtc ggcgctcagc gcgcgcagtt
    10801 cgtcgtcgct gaagccggcc tgtcgacggg cctcggtgtt gaacggcccg cgcaggcgcg
    10861 gggcgcggtg ctggcgcgtc agccggcggt agtgcgccag cggctccagg ccctggcgtg
    10921 cgcacagcca gcggtaccag tggttgccga tagccacatg gccgatttcc tcgcgcagga
    10981 tcaggtcgag gatgtcgcag gcggccagcg cgtcgggcgt ggccacggcg cgcagcttgc
    11041 gctggatcag cggcgtggcg tccaggccgc gcgcctccag cgtgcgcggc accagcgcca
    11101 tgcgggcggt gaggtcgtca cgggtttttt cgcacatcgc ccacagcccg tcgtgtgcct
    11161 cgaagtcgcc gtagcgcagc tggtggtggg tctgcagatg ggcgtgcagc aggctgaagt
    11221 ggtgcgattc ctcgatcgcc acgcgcaacc agtcgcggta atacgcctcg ggcatgccgg
    11281 caaagcgcca gatggcgtcc agcgccaggt tgatggcgtt gaattcgatg tggcagatgg
    11341 cgtgcaccag cgccgcacgg ccctcgcgcg tgtgcacgct gcgctgcggt acgcgcgccg
    11401 gatcgatcag cggcggccgc aagggacgac cgggcaggcc gggtggcgcc tcgagagcgg
    11461 tctcgggcgc tatgtaaagc gtagctgccg gcgcttgcag ggcggccacc agagccagtt
    11521 ttcgttccac atcggcttcg caaagggccg ccagggccag ggggcgcagg tccattcgta
    11581 gaattctatg tttcgcgcag cagcgagcga aggagaccag cgatggcggt gtatgagttg
    11641 gacggcgtgg cgccgcaggt ggatccggcg gcctggatcg ccgattcggc cgaggtgatc
    11701 ggcaacgtca gtctgggcgc cggcgccagc gtgtggttcg gctgcgtgct gcgcggcgac
    11761 accgaacgca tgatgatcgg cgagggcagc aacatccagg acctgagcgt gctgcatgcc
    11821 gacgtcggcc agccgctgac cgtcggccgc cacgtcaccg tcggccacaa ggtgatgctg
    11881 cacggctgca ccgtcggcga cgagtcgctg atcggcatcg gcgcggtggt gctcaacggc
    11941 gcccgaatcg gcaagaactg cctggtcggc gccggtgcgc tggtgaccga gggcaaggaa
    12001 tttccggacg gcagcatgat cgtcggcgcg ccggcgcggg tggtgcgcca gctcacgccc
    12061 gagcagatcg agggcctgcg ccagagcgcg cgccactacg tcgacaacgc gcggcgcttt
    12121 caggctggct tgaagcggct tggctgaagc cccatctgcc tccgatgtcc gaactgcaca
    12181 aattcatctt cgacggcctg ccggtgcgcg gccagctggt gcgcctgacc gacgcctggc
    12241 aggaggtgct gcgccggcgc gccgccaaca cccagaccgg cgcctggcca ccggtggtgg
    12301 ccgaaatgct gggcgagatg gccgcggcca ccgtgctgat gcagggcaac atcaagttcg
    12361 acggcgcgct gatcatgcag atcttcggcg acggcccgct caagctggcg gtggccgagg
    12421 tgcagcccga cctgcgcctg cgcgccaccg cctcggtggt gcagagcgtg acaccgggca
    12481 tggcgctgcc cgagctggtc aatcgccacg gccgcggccg ctgtgccatc acgctcgatc
    12541 cgcgtcgccg gcgcgaaggc cagcaggcct accagggcgt ggtgccactg gtggacgagg
    12601 gcggtgcgcc gttgcagacg ctggccagcg tgctggagca ctacatgcgc cagtccgaac
    12661 agctcgacac cacgctggtg ctggcggccg acgagcaggt ggccgccggt ttgctgatcc
    12721 agcgcctgcc ggtgcagggc caggacaacc tgtcgcaaag cagcgtggcg cgcgaggacg
    12781 ccggcagcgc ggccgacgac gactatcaac gcatccgcct gctggcgcac agcctgcagc
    12841 gcgccgagct gctgagcctg gatgccgaca ccctgttgca ccgcctgttc tggcaggagc
    12901 cgctgctgcg cttcgccccg ctggccggcc accaggggcc gcgctttgcg tgctcgtgca
    12961 gccgcgagcg agtggcggcg atgctgcagg ggctgggcca gcccgaggcc gaatcgatcc
    13021 tggccgaacg cggcgaaatc gaggtcggct gcgacttctg cggtcagcag cagcgcttcg
    13081 acgccgtcga tgtggcgcgc ctgttcaccc cggcggccca gcagtcgccg caaccggggt
    13141 cggtgcagtg agcgaccgcg ccggcggcgc gctgcgcttt gacgtcatca ccctgtttcc
    13201 tgaactgttt gctccgtttc tggaagctgg tgtcgcccgc cgggcttacg ccagtggcca
    13261 gatcgtcctg aaactgtgga acccgcgcgc gtacgccgag ggcaactacc gccgcgtcga
    13321 cgaccgtccc ttcggcggtg gccccggcat ggtgatgctg gccgagccgc tggcgcgctg
    13381 cctggcggcg gtgcaggccg agcgcgcggg cggcgccgcc cccgtggtgc tgttttcgcc
    13441 catcggccag accctgacac acgccggcgt cgagcgctgg gccgccggtg ccggcgccgt
    13501 gctgatctgc ggccgctacg agggcatcga ccagcgcttc atcgaccgcc atgtgacgca
    13561 ccagatcagc ttgggcgatt tcgtgctgtc gggcggcgaa atcgcggcac tggcgctgct
    13621 ggacgcggtg gcgcgcctgc agccgggggt gctgggcgat gagggcagcc accaccagga
    13681 cagtttcaac ctggccgtcg atggcctgct ggactgcccg cactacacgc ggcccgaggt
    13741 ctgggacggg gtgccggtgc cgtccgagct gctgtcggga caccatgcgc gcatcgaggg
    13801 ctggcggcgc gagcagcgct tgcgcctgac ggcgcgccac cgcccggacc tgatcgtggc
    13861 cgcgcgcgcc gccgggcgcc tgagcgcgca cgacgaggtg ctgctggccc agcttgggcg
    13921 cggcggacca gggtgatggc ctgaaggcga cggtcaggct ataatcaaag gcttttcgat
    13981 cctctgccag ccgcggcaca gattcaacac gctggatgca tgttcagtgt ggccgcccct
    14041 agcgtagcgt gcgcacgatc gagagatgag gaaaccatga acctgatcca gcagctcgaa
    14101 aaggaagaga tcgagcgcct cggcaaaagc atccccgagt tcgcccccgg cgacaccgtg
    14161 atcgtcagcg tcaacgtcgt cgaaggcaac cgcaagcgcg tgcaagccta cgaaggcgtg
    14221 gtgattgcca agcgcaaccg cggcctgaac agtggcttca cggtgcgcaa gatctccaac
    14281 ggcgagggcg tcgagcgcac cttccagacc tacagcccgc tgatcgccaa gatcgaagtc
    14341 aagcgccgcg gcgatgtgcg ccgtgccaag ctgtactacc tgcgcggcct gagcggcaag
    14401 aaggcgcgca tcaaggaaaa gctccccggc cgcgcggctt cgccgaccgc cgccgactga
    14461 tcggcagctt tgcgctcaca atcggaaccg cctgcggccc ctggctcagg cggttttttc
    14521 atgtccggac gaccgctggc gagctggccg gcatgtgcgc catgagtacg cccaccccgc
    14581 tgtccaagct gcccaacttc gatccgcgcc aggtgccggt gacgggaatc gatgcccatc
    14641 tgccgccggt gccggccgag gcgctgacgc tgcctgcgct gcgccggcgc ttccaggcgc
    14701 cgccgccgtg gcagcccgaa ctgcgccagg agccgcgctt caccgaccgc gtaccggccg
    14761 cggcggccgt gctggtgccg ctggtggacc ggccgcaggg gctgtcggtg ctgctgaccg
    14821 agcgttcggg gaacctttcc acccattccg gtcaggtggc ctttcccggt ggcaaggtcg
    14881 atccggagga tgccgacgcg gtggcggcgg cgctgcgcga agccgaagag gaggtcgatc
    14941 tgtcgcgccg cttcgtcgac gtgctggggc aactgccggt gtacgtgacc ggtacgcagt
    15001 tcatcgtcac gccggtggtg gcggtggttc agcccggctt cgtgctgcgc cccaacccgc
    15061 gcgaggtggc ccatgccttc gaggtgccgc tggcgtggct gatgaatccg gcccaccacc
    15121 gacgccatcg cctgacctgg gaggggcagg cgcgggagtg gttttccatg ccctacgtgg
    15181 agcccctgag cgtggtggcg ggcagtggcg agtcgctgcc gggcgcagcg gtggagcgct
    15241 tcatctgggg tgccaccgcc ggcatgttgc gcaacttcta cagatttcta tccgcctgag
    15301 ccgccctgcc tctggcacct gtcggtggct cactatcatc gggccatggc cttttttgcg
    15361 atcgcgctgg cgctgctgct ggagcaggtg cgtcccctga cgtcggacca tccggcagcg
    15421 gtcggcctgc accgctgggt gcgcctggtc gggcgcaacg tggacgccgg cgcggcccag
    15481 catggctggc tggcctgggt gctggcggtg gccgtgccgg cgctggcggt gagcgccgtg
    15541 cacctgctgc tgtcgtggct gggcggctgg ccgctggcgc tggtctggag cgtggccatg
    15601 ctctacctga cgttgggttt tcggcagttc agccaccatt tcaccggcat ccgggacgcc
    15661 ctggaagccg gcgacgagga gcgtgcccgc ggcctgctgg cgcactggca gcacgtggac
    15721 gcaagcacgg tgccgcgcag cgagatcgtg cgccatgtga tcgaggtttc ggtgctggcc
    15781 gcgcatcgcc atgtgttcgg tgtgctggcc tggttctgca tcggagcgct gctggggctg
    15841 gggccggccg gggcggtgtt cttccgcagc gccgagtacg tggcgcgtta ctggcgccgc
    15901 aaggcgcagg cggccgacca gcccagcagc ccggcgctgt gcctggcggc cgagcaggca
    15961 tggcagctca tcaactggct gccggcacgc accaccgccc tgggatttgc catcgtcggc
    16021 agcttcgagg acgcgatcga agtctggcgc agccacgcgt cgcgctttgc cgatcgcaac
    16081 gacggcgtca ttctggcggc cacggcgggt gcgctggggg tgcgactggg cggtgcctcg
    16141 ctgcggccgc tgacacccga gggcgccgcg gcggtgccgg tcagcgcggc cggcgtgtcg
    16201 ggcgaggggc tgcccggcga gccgccgcgc acggcccatt tcacccaggt ggtgggcctg
    16261 gtgtggcgca ccgtggcgct gtggctgctg ttgctggtgc tgctgacact ggcccatgtg
    16321 ctgggctgag caggtgtgaa cgaccccgcg tgcccgctcg gccgctccgg ctcaccg
//
LOCUS       DAOQTZ010000065        15894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4188_length_15894_cov_13.914767, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000065 DAOQTZ010000000
VERSION     DAOQTZ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 15894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15894
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4188_length_15894_cov_13.914767"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKN51_08855"
     CDS             <1..581
                     /locus_tag="PKN51_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT85248.1"
                     /translation="LQVMDHGTLTDNNGRKADFRNVIIIMTTNAGAETMNKATIGFTT
                     QRQQGDEMADIKRLFTPEFRNRLDAIVSFKPLDEQVILRVVDKFLLQLEQQLAEKKVE
                     VTFTDKLRKFLAKKGFDPLMGARPMQRLIQDTIRKALADELLFGRLTEGGRLTVDLDD
                     KDEVLLDITPLPKKERARGAEPAEPEEEIATD"
     gene            complement(640..1617)
                     /locus_tag="PKN51_08860"
     CDS             complement(640..1617)
                     /locus_tag="PKN51_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT85249.1"
                     /translation="MNTPTFAADRRAHHLIVGDGVVGRATAQALHAQGQQVVLASRHA
                     PAAGATEATHLTVDALDAPSLRRAAEGASHLYLTLGLAYRIGVWQRDWPRIMHNAIQA
                     ALASGARLLWFDNVYLYGTPLQVPMRENHPIDPPSHKGRVRARLLAMLLEAEDRHGLR
                     WLVARSADFYGPDVRLSILYAAAMERQLQRRAAWWLGDPDRRHSFTYVPDAGRALARL
                     ALDDGTWQQSWHLPTASPAPTPRELLTESARLLGAPTEVRALPVALVRTLGLAVPLLR
                     EVGEMLYQNQQDYVFSSEKFMARYPDFRITPYAEGLTAMIASLRPAART"
     gene            1745..3193
                     /gene="sbcB"
                     /locus_tag="PKN51_08865"
     CDS             1745..3193
                     /gene="sbcB"
                     /locus_tag="PKN51_08865"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HNT85250.1"
                     /translation="MSASTFLWHDYETFGTRPRRDWPAQFAAVRTDAELNEIGEPLVL
                     YCQPPTDRLPEPEACLITGITPQECAAKGLPEHEFARRIHAALAQPGTIGVGYNSIRF
                     DDEVTRFLLWRNLIEPYGREWRDGCSRWDLLDVMRCAHALRPEGIEWPQGEDGLTTFK
                     LERLSAANGLAHEAAHDALSDVRATVALARLLRQCQPRLFDFCLALRSKDRVAQELGL
                     PTTLRDARPFLHVSGRFGAARGCIAVMAPLAMHPTNKNELLAWDLSQDPRELADLQPE
                     QVRARLFTSTADLPDGTTRLPIKGVHLNKSPIVVGQIKTLRPELAERWGIDFGVAASH
                     LDHLRALPDLSALWPQVYARPGLAEPVDVDEDLYGGFLGDADRRRLDRLRALEPGELA
                     HAHTGFDDERLPELLLRYRARNFPHTLNEAERARWLAHRTGRLMDGEGGALTIAALFD
                     RIDALAETVHDERGQAVLEALYAWAEHVAPEG"
     gene            complement(3489..4064)
                     /locus_tag="PKN51_08870"
     CDS             complement(3489..4064)
                     /locus_tag="PKN51_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNT85251.1"
                     /translation="MPSLSPAPPPTGRARRRAWLARAARDGLLLLALLWAVQAWQTRD
                     VPSGAAPDFSAAAVLPPDRDAAMLSLAQWRAAHRGRPVALHFWAEWCGICRLEQHSVT
                     RLAADWPVLTVATQSGAAPAVHAVQARRELPWATVADPDGTLLTRYGLKAVPAFVVVA
                     PDGRLSSVSVGYTSEAGMRLRLWWAGLWAGG"
     gene            4170..5534
                     /locus_tag="PKN51_08875"
     CDS             4170..5534
                     /locus_tag="PKN51_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNT85252.1"
                     /translation="MSTPTKYLLQESQIPKFWYNIQADLPKPMPAVLHPGTLQPIGPA
                     DLAPLFPMAIIQQEVATEREIEIPEPVREVFRLWRPAPLFRAHRLEKALGTPAKIFYK
                     YEGVSPAGSHKPNSAIPQAFYNAQEGVKRLSTETGAGQWGSSLALAGQIFGLDVTIYQ
                     VRVSYDQKPYRRALMETYGARCVASPSNETEYGRAVLAQRPDHPGSLGIAISEAVEVA
                     AKNDDTKYALGSVLSHVLLHQTIIGLEAMEQMHMADAWPDVLVGCTGGGSNFAGLTFP
                     FIGHGLRGGAMPRVVAVEPAACPSLTRGKYAYDFGDTAHLTPLTKMHTLGSTFTPPGF
                     HAGGLRYHGMAPLVSHVKELGLLEAVAYTQGECFAAGVQFARAEGIVPAPEANHAIKG
                     AIEEALRCKREGKAETILFNLSGHGHFDMGAYQAYFTGQLKDEAYDEKELAQALSGLP
                     AVPA"
     gene            complement(5554..6600)
                     /locus_tag="PKN51_08880"
     CDS             complement(5554..6600)
                     /locus_tag="PKN51_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNT85253.1"
                     /translation="MGLKQRAAQAGRWLAGVLFGVLGLTSMAGAQPVAVSSFAEGQGN
                     GVYAFASSTPKTLGELMQGGKEPVNILGHLFLPAGADKVPAVILIHGSGGVYPALLEY
                     WAKQFNGAGMAAFVIDMFGPRGVGSTADDQSQVPFSADVADSFAALRLLATHPRIDRQ
                     RIAVMGFSRGGTAVLRSAVERLIASQKLPDGLRYAAFIPTYAGGCSNIFRLVVRPGVF
                     SKAPMLFIHGTADDYTPIQPCQDYADRIRAAGTPVEFVAIEGAHHKFDSDDTRRHFLA
                     RASKTSADCPIEVDIDNFQAFDRQTGARLQGEAYNTALKNCRAMGANVEGNKKARDQA
                     AEAALSFLKKTFAM"
     gene            complement(6761..7501)
                     /gene="serB"
                     /locus_tag="PKN51_08885"
     CDS             complement(6761..7501)
                     /gene="serB"
                     /locus_tag="PKN51_08885"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNT85254.1"
                     /translation="MTSQTALDASNEISPGLVLRGIQPPLRLSDYRLIAFDMDSTLIN
                     IECVDEIADAVGRKAEVAAITEAAMRGEITDFKDSLRRRVALLRGVSVDELAQVYRAR
                     LRLNPGAAELVLACKAAGLKVLLVSGGFSYFADRLRDRLALDFARSNLLEIEHGRLTG
                     RIQPQSWGDICDGAMKRETVLSTCSLIGCSPSQAIAMGDGANDLEMMRAVGLSVAYRA
                     KPRVRAQARVAIDMGGLDRLLEVFGAQV"
     gene            complement(7575..8210)
                     /locus_tag="PKN51_08890"
     CDS             complement(7575..8210)
                     /locus_tag="PKN51_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNT85255.1"
                     /translation="MNPSWPSRRLWHQLLWLGALALLAAPALADEALARRFAAAALRA
                     GDTGGRAFAVVSKPAATVWVFDRHGQLLASSAVLLGQATGDAAPPDVGSRPLHQLRPH
                     EKVTSAGRFLTEAGRNHRGEDIVWLDYDAALSMHRVRQVAGEQRPQRLQTPGVADNRI
                     SYGCINVPDRFYDRTIDPLFARQSGVVYVLPETEPIERWFAFARDLDAAAP"
     gene            complement(8229..11711)
                     /gene="mfd"
                     /locus_tag="PKN51_08895"
     CDS             complement(8229..11711)
                     /gene="mfd"
                     /locus_tag="PKN51_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNT85256.1"
                     /translation="MHLPKLIPGKRHALPRPPASSDALLLARLAQREAAEQRLVAIVT
                     ADAGDARRLLDELGFFAPDLRVVLFPDWETLPYDTFSPHQDLISERLATLWAIRQGQA
                     DVVLLPATTALYRLAPPAFLAAHTFQFRQGQRLDEAALRSQLTLAGYQHVSQVVSPGE
                     YAVRGGLIDLYPMGSPMPYRVDLFDDEIDSIRGFDPDTQRSLYPVPEVRLLPGREFPL
                     DEAARTRFRQRWRELLEGDPTRSRIYKDMAGGVATAGIEYYLPLFFDHTATVFDYLGS
                     GATLVLHGELETSFQQFSQDTRERWRLAQGDPERPVLPPEALFLNAEQFYLQAKACPQ
                     VALRPGVDDIEHSAFAQKLPPLTVVRGADDPLARLQAHLRGAARRLLVLAESDGRRQS
                     LLDFLRASGLNPPAFDTLAEFEASTEPVGIATAALAEGFAWVEGGIDFVTETELFATA
                     PGTRRRRRQEQVSDVEALIKDLSELNVGDPVVHTQHGIGRYRGLVHLDMGEKNADGTP
                     ALQEFLHLEYAQQAVLYVPVSQLHLIGRYTGVSADEAPLHRLGSGQWDKARRKAAEQV
                     RDTAAELLNLYARRAARQGHAFRYSPHDYEQFAADFGFEETADQQAAIHAVIQDLISP
                     RPMDRLVCGDVGFGKTEVALRAAFVAVTGGKQVAFLAPTTLLAEQHYQTLVDRLARWP
                     IKVAQMSRFASGKETAAALKGLADGSVDIVVGTHKLLSEKTQFKNLGLLIIDEEHRFG
                     VRHKEAMKKFRAEVDVLTLTATPIPRTMGMALEGLRDLSVIATAPERRLAIKTFVRDE
                     GAGVIREAVLRELKRGGQVYFLHNEVETIENRRQALEKLLPEARIAIAHGQMPERELE
                     RVMRDFVAQRFNLLLCSTIIETGIDVPTANTIVIARADRFGLAQLHQLRGRVGRSHHQ
                     AYAYLLVPDQQSLTKQAAQRLEAIQQMEELGSGFYLAMHDLEIRGAGEVLGENQSGNM
                     MEIGFQLYNEMLAEAVRSLKAGREPDLLAPMQAATDINLHAPALLPHDYCGDVHLRLS
                     FYKKLATARTGEQIDALVEEIVDRFGKLPAPAQTLIDVHRLRVLSAPYGVQKVDAAPG
                     VISIAFKPNPPIDPMAIIHLIQKNRHIKLAGNDKLRIERALPDPQARAQLVRDVLRSL
                     GQPIQPPLAASAPA"
     gene            11924..14263
                     /locus_tag="PKN51_08900"
     CDS             11924..14263
                     /locus_tag="PKN51_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNT85257.1"
                     /translation="MSPTDDRPIQPQRYGSGQAVRRLEDDALLQGQGRFTDDLGAPGD
                     GWLVFVRSPHAHAALRSVDAEAARALPGVRAVITGADLVAAGMPPLPTGAPFRRADGS
                     ACATPPRRALAHEVVRHVGEAVAAVVADSLAQARDAADALVVDYRELPVVASLAAALR
                     ADAVVTPAAPDNIACEARHGDAAAAQAAFAQAAHVVALDLTHQRLAALTLEPRAVRAA
                     WDAARGRLDMHASTQMPTALRATVASALGLTPEAVRVRVGDVGGGFGMKTGAYPEDVV
                     VAWAARHTGRPVRWSGERGEEFLSTTHGRDVQAHAELALDAEGRMLALRVHSHANVGA
                     YALATGVAIQLLIGPWVQTSVYHVPVIDFHFRAVLTHQAPTGAYRGAGRPEAIFNIER
                     LLDEAARQSGIDRIALRRRNFIRPEQMPYTNPMGQTYDVGRFERVLDQALPLADWDGF
                     AARAQQSRARGLWRGLGIATFLEWTGGNAFEESVTLEVKSDGIVEVFSAVNQMGQGIA
                     TSLAQLVVDVLQVPIERVRVVLGDTERGNGFGSAGSRSLFTGGSAMRGGAEHTLAQAH
                     QLAASALEAAPEDLRYQAARFHVVGTDHAIGLFELAGRQPGAHIQVEHTHTVAGPSWP
                     NGAHICEVELDPQTGAVAVVGYTSVNDVGRVVNPLIVRGQLDGGAVQGIGQALCEQIL
                     YDAASGQLLTGSLMDYAVPRAHELATCFRTEMDESTPCANNALGVKGVGELGTIGATP
                     AVVNAVADALARAGRPELAPRLQMPLSPARLWALLQSQG"
     gene            14458..15162
                     /gene="ispD"
                     /locus_tag="PKN51_08905"
     CDS             14458..15162
                     /gene="ispD"
                     /locus_tag="PKN51_08905"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNT85258.1"
                     /translation="MSVQSPRCHALVPCAGSGARAGGVQPKQYQLVAGQPLVRHTLAA
                     LASVARLVRVLVVVAPGDDRLHELPAPLEVAPCGGATRAESVLNGLRHLRATGAADSD
                     WVLVHDAARCLITPAQVDALIDACWQDAVGGLLALPLPDTLKMEDAQGRSAATVERAG
                     KWLAQTPQMFRLGALHDALAAHAARGFAGITDEASAIEAAGLRPLLVCGSAHNVKVTY
                     PEDFALAEAVLRHRTP"
     gene            15159..15662
                     /gene="ispF"
                     /locus_tag="PKN51_08910"
     CDS             15159..15662
                     /gene="ispF"
                     /locus_tag="PKN51_08910"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNT85259.1"
                     /translation="MTDAEPSRLPALRIGEGWDVHALVPGRPLVIGGVTVPHTHGLLG
                     HSDADVLLHAVTDALLGAAGLGDIGRHFPDTDARFAGADSAVLLAEAARRVGAAGWRV
                     GNVDGTVIAQAPKLAPHLPAMVARIAQVLGVGVAQVNVKAKTAERLGPVGQGASIEAR
                     AVVLLFR"
BASE COUNT         2352 a         5541 c         5669 g         2332 t
ORIGIN      
        1 tgctgcaggt gatggaccac ggcacgctga ccgacaacaa cgggcgcaag gccgacttcc
       61 gcaacgtcat catcatcatg accaccaacg cgggcgccga gaccatgaac aaggcgacca
      121 tcggcttcac cacccagcgc cagcaaggcg acgagatggc cgacatcaag cgcctgttca
      181 cgcccgagtt ccgcaaccgc ctggacgcca tcgtcagctt caagccgctg gacgagcagg
      241 tcatcctgcg cgtggtcgac aagttcctgc tgcagctgga gcagcagctg gccgagaaga
      301 aggtcgaagt caccttcacc gacaagctgc gcaaattcct ggccaagaag ggcttcgatc
      361 ccctcatggg cgcgcgcccg atgcagcgcc tgattcagga caccatccgc aaggcgctgg
      421 ccgacgagct gctgttcggc cgcctgaccg agggcgggcg cctgacggtg gacctggacg
      481 acaaggacga ggtgctgctc gacatcacgc cgctgccgaa gaaggagcgt gccaggggcg
      541 ccgagccggc cgagccggaa gaagagatcg ccaccgactg aggtcgagcc tgcgttcacc
      601 cgaccagccc gctgccacgc agcgggcttt tttgtgggtt caggtgcgcg ccgcggggcg
      661 cagcgaggcg atcatcgccg tcagcccctc ggcgtagggc gtgatgcgaa agtcggggta
      721 gcgcgccatg aacttctcgc tgctgaagac gtagtcctgc tggttctggt aaagcatttc
      781 accgacttcg cgcagcagcg gcacggccag cccgagcgtg cgcaccaggg ccaccggtag
      841 ggcgcgcact tcggtgggcg cccccagcag gcgtgcgctt tcggtcagca gttcgcgggg
      901 cgtcggtgcc gggctggcgg tgggcaggtg ccagctttgc tgccaggtgc cgtcatccag
      961 ggccaggcgc gccagcgcgc ggccggcgtc cggaacgtag gtgaagctgt ggcgccggtc
     1021 ggggtcgccc agccaccatg ccgcgcgcct ttgcaactgg cgctccatgg cggccgcgta
     1081 caggatcgac aggcgcacgt ccgggccata gaagtcggcg ctgcgcgcca ccagccagcg
     1141 caggccatgg cgatcctcag cctccagcag catggccagc aggcgcgccc gcacgcggcc
     1201 cttgtgcgac ggtggatcga tcggatggtt ttcccgcatc ggtacctgca gcggtgtgcc
     1261 gtagaggtag acgttgtcga accacagcag ccgcgcgccg ctggccagcg ccgcctggat
     1321 ggcgttgtgc atgatgcgcg gccagtcgcg ctgccacacg ccgatccgat aggccaggcc
     1381 gagcgtcaga tacaggtggc tggcgccctc ggcggcacgg cgcagggaag gcgcgtccag
     1441 cgcgtcgacc gtcaggtggg tggcctcggt ggcacctgcc gccggcgcat gtcggctggc
     1501 cagcaccacc tgctggccct gcgcgtgcag cgcctgtgcg gtggcgcggc cgaccacgcc
     1561 atcgcccacg atcagatggt gcgcgcggcg atcggcggcg aaggtggggg tgttcatgtc
     1621 gccatcatga accgtcggag gtggcggcgg catgctgtgg gacaaagcaa tcgttccaag
     1681 atgcatcagg ccccgcaccc gccagggggc gcgcagccgg gctggccatt atgcttgcca
     1741 ccccatgagc gcctccactt tcctctggca cgactacgaa accttcggca cccgcccgcg
     1801 ccgcgactgg ccggcccagt tcgccgccgt gcgcaccgat gccgagctga acgagatcgg
     1861 cgagccgctg gtgctgtact gccagccgcc gaccgaccgc ctgcccgagc ccgaggcctg
     1921 cctgatcacc ggcatcacgc cgcaggagtg cgccgccaag gggctgcccg agcacgaatt
     1981 tgcccgccgc atccacgccg cgctggccca gccgggcacc atcggcgtgg gctacaactc
     2041 gatccgcttc gacgacgagg tgacgcgctt tctgctctgg cgcaacctga tcgagcccta
     2101 tggccgcgag tggcgcgacg gttgcagccg ctgggacctg ctggacgtga tgcgctgcgc
     2161 ccacgccttg cgacccgagg gcatcgagtg gccgcagggc gaggacggtc tgaccacctt
     2221 caagctggag cgcctgagcg ccgccaacgg tctggcgcac gaagcggcgc acgacgcgct
     2281 gtccgacgtg cgcgccaccg tggcgctggc gcgcctgctg cgccagtgcc agcccaggtt
     2341 gttcgacttc tgcctggccc tgcgcagcaa ggaccgcgtg gcccaggagc tgggcctgcc
     2401 caccaccttg cgtgacgcgc ggccgtttct gcatgtgagc ggtcgcttcg gcgccgcgcg
     2461 aggctgcatc gccgtgatgg cgccgctggc catgcacccg accaacaaga acgagctgct
     2521 ggcctgggac ctgagtcagg atccgcgcga actggccgac ctccagcccg agcaggtgcg
     2581 cgcgcgcctg ttcaccagca ccgccgattt gcccgacggc acgacgcggc tgcccatcaa
     2641 gggcgtgcac ctgaacaaat cgcccatcgt ggtcggccaa atcaagaccc tgcgccccga
     2701 gctggccgag cgctggggca tcgacttcgg cgtcgccgcc agccacctgg accacctgcg
     2761 tgcgctgccc gacctgagcg cgctgtggcc gcaggtttat gcgcgccctg gcttggccga
     2821 gccggtggat gtggacgaag acctgtacgg cggcttcctg ggcgacgccg atcgccgccg
     2881 cctggaccgc ctgcgcgccc tggagcctgg cgagctggcc cacgcgcaca ccggcttcga
     2941 tgacgagcgc ctgcccgagc tgctgctgcg ctaccgtgcg cgcaacttcc cgcacacgct
     3001 gaacgaggcc gagcgcgccc gctggctggc gcatcgcacc ggccggctga tggacggcga
     3061 gggcggcgcg ctcaccatcg ccgcgctgtt cgaccgcatc gacgcgctgg ccgagacggt
     3121 gcacgatgag cgcggccagg cggtgctgga agccctgtac gcatgggccg aacacgtcgc
     3181 tcccgaaggc tgacatcggg cgttgacgaa cgacggatcg aatgacgaat gagctcgctg
     3241 cgcctcgatg gccgatcgat cacaggccat gccgcgcgtc gccggcgtca tgcatcatcc
     3301 ctctgaacat cagccggaat actatcaaaa caatagcttg cggcgcttga ccagcaagcg
     3361 ccaaagccct gtttgactcc gatgttcagg agcgcgcgct ggcgacgccg aacccatcga
     3421 aagcaggagg gatgtttccc tgcgtccttc gcgtccggca gtgcaacaga ccggcacccc
     3481 ggcccagatc agccgccggc ccacaggccg gcccaccaca gccgcagccg catcccggct
     3541 tcgctggtgt agccgacgct gacgctggac aggcgcccgt cgggtgcgac gaccacgaag
     3601 gccggcaccg ccttcaggcc gtagcgcgtc agcagcgtgc cgtcggggtc ggccaccgtg
     3661 gcccagggca gttcgcgtcg ggcctgcacg gcgtgcactg ccggcgccgc tccggattgg
     3721 gtggccaccg tcagcaccgg ccagtcggcg gccaggcgcg tcacgctgtg ctgctccagc
     3781 cggcagatgc cgcaccactc ggcccagaaa tgcagcgcca ccggccggcc gcgatgggcg
     3841 gcccgccact gcgccaggct cagcatggcc gcgtcgcggt ccggcggcag cacggcggcg
     3901 gcgctgaaat cgggcgctgc gccgctgggc acgtcgcgcg tctgccacgc ctgcacggcc
     3961 cacagcaagg ccagcagcag caggccgtcg cgcgccgcgc gtgccagcca ggcgcgccgg
     4021 cgcgcgcggc cggtcggcgg cggggcgggg gatagggaag gcacggtgcg gtgggcgacg
     4081 gcgaaaccga tagtattgac ggtttcccgc cgcccagcgt tttgccgccg ggccgcggtc
     4141 ccgtcccacc ctcacccagg agcgcgccaa tgagcacacc gaccaagtac ctgctgcagg
     4201 aaagccagat ccccaagttc tggtacaaca tccaggccga tctgcccaag cccatgccgg
     4261 cggtgctgca ccccggcacc ctgcaaccga tcgggccggc ggacctggca ccgctgtttc
     4321 cgatggccat catccagcag gaggtggcca ccgaacgcga gatcgagatc cccgagccgg
     4381 tgcgcgaggt gtttcgcctg tggcgtccgg cgccgctgtt tcgcgcccac cggctggaaa
     4441 aggccctggg cacgccggcc aagatcttct acaagtacga gggcgtcagc ccggccggca
     4501 gccacaagcc caattcggcc atcccgcagg ccttctacaa cgcgcaagag ggcgtcaagc
     4561 gcctgtcgac cgagaccggc gccggccagt ggggcagctc gctggcgctg gctggacaga
     4621 ttttcggcct cgacgtcacc atctaccagg tgcgtgtcag ctacgaccag aagccctacc
     4681 gccgcgccct gatggaaacc tacggtgcgc gctgcgtggc ctcgcccagc aacgaaaccg
     4741 agtacggccg tgccgtgctg gcccagcggc cggaccaccc gggcagcctg ggcatcgcca
     4801 tcagtgaggc ggtcgaggtg gcggccaaga acgacgacac caagtacgcc ctgggctcgg
     4861 tgctgagcca cgtgctgctg caccagacca tcatcggcct ggaggcgatg gagcaaatgc
     4921 acatggccga cgcctggccc gacgtgctgg tgggctgcac cggcggcggc agcaacttcg
     4981 ccggcctgac cttccccttc atcggccatg gcctgcgcgg cggtgccatg ccgcgcgtgg
     5041 tggcggtcga gcccgccgcc tgccccagcc tgacgcgcgg caagtacgcc tacgacttcg
     5101 gcgacaccgc gcacctgacg ccgctgacca agatgcacac cctgggcagc acgttcacgc
     5161 cgccgggctt tcacgccggc ggactgcgct accacggcat ggcgccgctg gtcagccacg
     5221 tcaaggaact gggcctgctg gaggccgtgg cctacaccca gggcgagtgc tttgccgccg
     5281 gcgtgcagtt tgcgcgcgcc gaaggcatcg tgccggcacc cgaagccaac cacgccatca
     5341 agggcgccat cgaagaggcg ctgcgctgca agcgcgaagg caaggccgaa accatcctgt
     5401 tcaacctgag cggccacggc cacttcgaca tgggcgccta tcaggcctac ttcaccggcc
     5461 aactgaagga cgaggcctac gacgaaaagg aactggcgca ggcgctgtcg ggcctgccgg
     5521 cggtgccggc ctgacggcgc ggggcgcgcc ccgtcacatg gcgaaggttt tcttcaggaa
     5581 gctcagtgcc gcctcggcgg cctgatcgcg cgctttcttg ttgccttcca cgttggcgcc
     5641 catggcgcgg cagttcttca gagccgtgtt gtaggcctcg ccctgcagcc tggcgccggt
     5701 ctggcggtca aaggcctgga aattgtcgat gtcgacctcg atcgggcaat cggcgctggt
     5761 cttggaggca cgcgccagga agtggcgccg ggtgtcgtcg ctgtcgaact tgtggtgcgc
     5821 gccctcgatg gcgacgaatt cgaccggcgt gccggcggcg cggatcctgt cggcatagtc
     5881 ctggcagggc tggatcggcg tgtagtcgtc ggccgtgccg tgaatgaaca gcatcggcgc
     5941 cttggagaac acgcccggcc gcacgaccag gcggaaaatg ttggagcagc cgccggcata
     6001 ggtcgggatg aaggcggcgt agcgcagacc gtcggggagt ttctgcgagg cgatcaggcg
     6061 ctccacggcg ctgcgcagca ccgccgtgcc gccgcgcgaa aagcccatga ccgcgatgcg
     6121 ctggcggtca atgcgtgggt gggtggccag caaccgcagc gcggcaaagc tgtcggcaac
     6181 gtcggcgctg aacggcacct gcgactggtc gtcggcggtg ctgccgacgc cgcgcggccc
     6241 gaacatgtcg atcacgaacg ccgccatgcc ggcgccgttg aactgcttgg cccagtattc
     6301 cagcagggcc gggtagacgc ccccggaacc atggatcagg atgacggccg gcaccttgtc
     6361 ggcgccggcg ggcaggaaca gatggccgag gatgttgacc ggctccttgc cgccctgcat
     6421 cagctcgccc agcgtcttgg gcgtcgagct ggcgaacgca tacaccccgt tgccctggcc
     6481 ctcggcaaag ctgctgacgg ccaccggctg cgcccccgcc atggacgtca ggcccagcac
     6541 accgaacagc acccctgcga gccacctgcc ggcctgcgcc gcgcgttgtt tcagacccat
     6601 gatgcgcctc catcgaagca tcggtggcgg cagtgtacgg catcccgtgc gcaatggcac
     6661 ccagaaaacg acgaatggcg atcgcgccgg gcgtcgatcg cggcggggtg gtggcggatg
     6721 tcgtgtgccg ccggcggccg ttgcgcggcc gcgcggcgcc tcagacctgg gcgccgaaca
     6781 cttccagcag ccggtccagc ccgcccatgt cgatcgccac cctggcctga gcgcgcacgc
     6841 ggggcttggc ccggtaggcc accgacaggc cgacggcgcg catcatctcc aggtcgttgg
     6901 cgccgtcgcc catggcgatc gcctggctgg gcgagcagcc gatcaggctg caggtggaca
     6961 gcaccgtctc gcgcttcatg gcgccgtcgc agatgtcgcc ccagctctgc ggctggatgc
     7021 gcccggtcag gcgcccgtgc tcgatctcca gcaggttgct gcgcgcgaag tccagcgcca
     7081 gccggtcgcg caggcggtcg gcaaagtagc tgaagccacc cgacaccagc agcaccttca
     7141 gcccggccgc cttgcaggcc agcaccagct cggcggcgcc tgggttcagg cgcaggcggg
     7201 cacggtagac ctgcgccagc tcgtccaccg acacgccccg cagcagcgcc acgcgccggc
     7261 gcaggctgtc cttgaagtcg gtgatctcgc cgcgcatggc cgcttcggtg atggcggcca
     7321 cctcggcctt gcggcccacg gcgtcggcga tctcgtccac gcattcgatg ttgatcagcg
     7381 tggagtccat gtcgaaggcg atcagtcggt agtccgacag gcgcagcggg ggctggatgc
     7441 cgcgcaacac caggccgggg ctgatttcgt tcgatgcgtc aagggcggtc tgggaggtca
     7501 tgaactactg aaagaatagc tgctggcgct tgctggacaa gcgctggagg ccaaaataat
     7561 gctgaatcga ggggtcatgg ggcggccgcg tccagatcgc gtgcgaacgc aaaccagcgc
     7621 tcgatcggct cggtttccgg cagcacatag accacgcccg actggcgcgc aaacagcggg
     7681 tcgatggtgc ggtcgtagaa gcgatccggc acgttgatgc agccatacga aatgcggttg
     7741 tcggccacgc cgggcgtctg caggcgctgc gggcgctgct cgcccgccac ctggcgcacg
     7801 cggtgcatcg acagcgcggc gtcgtagtcc agccagacga tgtcttcgcc gcggtggttg
     7861 cgccccgcct cggtgaggaa gcggccggcg ctggtgacct tttcgtgcgg gcgcagctgg
     7921 tgcagcgggc ggctgccgac gtccgggggc gcggcgtcgc cggtggcctg acccagcagc
     7981 acggcgctgc tggccagcag ctggccgtgg cggtcgaaca cccagaccgt ggcggccggc
     8041 ttgctgacca ccgcgaaggc gcgcccgccg gtgtcgccgg cgcgcagcgc ggcggcggca
     8101 aagcggcggg ccagggcttc gtcggccagc gcgggcgcgg ccagcagcgc cagcgcgccc
     8161 agccacagca gctggtgcca gaggcggcgg gaaggccagg acgggttcat gggtggcgcc
     8221 attctggcct atgccggggc gcttgcggcc agcggcggct ggatcggttg cccgaggctg
     8281 cgcagcacgt cgcgcaccag ctgcgcgcgc gcctgcgggt cgggcagggc gcgttcgatg
     8341 cgcagcttgt cgttgccggc cagcttgatg tggcggttct tctggatcag atggatgatg
     8401 gccatgggat cgatcggcgg gttgggcttg aaggcgatgc tgatcacgcc cggcgcggcg
     8461 tccaccttct gcaccccata cggggcgctc agcacgcgca ggcggtgcac gtcgatcagg
     8521 gtctgcgccg gggcgggcag cttgccgaag cggtcgacga tttcttccac cagggcgtcg
     8581 atctgctcgc cggtgcgcgc cgtcgccagc ttcttgtaga aggacaggcg caggtgcacg
     8641 tcgccgcaat agtcatgggg cagcagggcg ggggcgtgca ggttgatgtc ggtggccgcc
     8701 tgcatgggtg ccagcaggtc gggctcgcgc ccggccttga ggctgcgcac ggcctcggcc
     8761 agcatctcgt tgtagagctg aaagccgatt tccatcatgt tgccgctctg gttttcgccc
     8821 agcacctcgc cggcgccgcg gatttccagg tcgtgcatgg cgaggtaaaa gcccgaaccc
     8881 agctcctcca tctgctggat cgcctccagc cgctgggcgg cctgcttggt caggctctgc
     8941 tggtcgggca ccagcaggta ggcgtaggcc tggtggtggc tgcgccccac gcggccgcgc
     9001 aactggtgca gctgcgccag gccgaagcgg tcggcgcgcg caatgacgat ggtgttggcg
     9061 gtgggcacgt cgatgccggt ttcgatgatg gtcgagcaca gcagcaggtt gaagcgctgc
     9121 gccacgaagt cgcgcatcac gcgctcgagc tcgcgctcgg gcatctggcc gtgggcgatg
     9181 gcgatgcgcg cctcgggcag cagcttttcc agcgcctggc ggcggttttc gatcgtctcg
     9241 acctcgttgt gcagaaagta cacctggccg ccgcgcttca actcgcgcag caccgcctcg
     9301 cggatcacgc ccgcgccttc gtcgcgcacg aaggtcttga tggccaggcg gcgctcgggc
     9361 gcggtggcga tcacgctcag gtcgcgcagg ccttccagcg ccatgcccat ggtgcgcggg
     9421 atcggcgtgg cggtgagcgt gagcacgtcg acctcggcgc ggaacttctt catcgcctcc
     9481 ttgtggcgca cgccgaagcg gtgttcctcg tcgatgatga gcaggcccag gttcttgaac
     9541 tgggtctttt ccgacagcag cttgtgcgtg ccgaccacga tgtccacgct gccgtcggcc
     9601 aggcccttga gcgcggcggc agtttccttg ccggaggcaa agcggctcat ctgcgccacc
     9661 ttgatgggcc agcgggccag gcgatcgacc agcgtctggt aatgctgctc ggccagcagc
     9721 gtggtgggcg ccagaaacgc cacctgcttg ccgccggtga ccgccacgaa ggcggcgcgc
     9781 agagcgactt cggtcttgcc gaagccgacg tcgccgcaca ccagccggtc catcggccgc
     9841 gggctgatca ggtcctggat cacggcgtgg atggcggcct gctggtcggc ggtttcctcg
     9901 aaaccgaagt cggccgcgaa ctgctcgtag tcgtgcggcg aatagcggaa cgcatggccc
     9961 tggcgcgcgg cgcgtcgggc gtacaggttg agcagctcgg cagcggtgtc gcgcacctgc
    10021 tcggcggcct tgcgccgcgc cttgtcccac tggccgctgc ccagccggtg cagcggcgcc
    10081 tcgtcggcac tgacgccggt gtagcggccg atcaggtgca gctgcgacac cggcacgtac
    10141 agcacggcct gctgcgcgta ttccaggtgc aggaattcct gcagcgccgg cgtgccgtcg
    10201 gcgttcttct cgcccatgtc caggtgcacc aggccgcggt agcggccgat gccgtgctgc
    10261 gtgtgcacca ccgggtcgcc gacgttgagc tcggacaggt ccttgatcag cgcctcgacg
    10321 tcgctgacct gctcctgccg ccggcgccga cgcgtgccgg gggcggtggc gaacagctcg
    10381 gtttcggtga cgaagtcgat gccgccttcg acccaggcaa agccctcggc cagcgcggcg
    10441 gtggcgatgc cgaccggctc ggtgctggcc tcgaattcgg ccagcgtgtc gaaggccggc
    10501 gggttcaggc cgctggcgcg cagaaagtcc agcaggctct ggcgccggcc gtcgctttcg
    10561 gccagcacca gcaggcgccg cgcggcgccg cgcaggtgcg cctgcaggcg cgccagcggg
    10621 tcgtcggcgc cgcgcaccac ggtcagcggc ggcagcttct gggcaaaggc gctgtgctcg
    10681 atgtcgtcca cgcccgggcg cagcgccacc tgcgggcagg ccttggcctg cagatagaac
    10741 tgctcggcgt tcaggaacag cgcctcgggc ggcagcaccg ggcgttccgg atcgccctgg
    10801 gccaggcgcc agcgctcgcg cgtgtcctgg ctgaactgct ggaagctcgt ctccagctcg
    10861 ccatgcagca ccagcgtggc gccgctgccg agatagtcga acaccgtggc cgtgtggtcg
    10921 aagaacagcg gcaggtagta ctcgatgccg gcggtggcca cgccgcccgc catgtccttg
    10981 tagatgcggc tgcgcgtcgg atcgccttcc agcagctcgc gccagcgctg gcgaaagcgt
    11041 gtgcgcgccg cctcgtcgag cgggaactcg cgccccggca gcagccgcac ctcgggcacc
    11101 gggtacaggc tgcgctgggt gtcggggtcg aagccgcgga tggagtcgat ctcgtcgtcg
    11161 aacagatcga cccggtaggg catggggctg cccatcgggt agaggtcgat cagaccgccg
    11221 cgcaccgcgt actcgcccgg gctgaccacc tggctgacgt gctggtagcc ggccagcgtg
    11281 agctggcttc tgagcgccgc ctcgtcgagc cgctgcccct gccggaactg gaaggtgtgg
    11341 gcggccagaa aggccggcgg cgccagccgg tacagcgccg tggtggccgg cagcagcacc
    11401 acgtcggcct ggccctggcg gatcgcccac agcgtggcca ggcgctcgct gatcaggtcc
    11461 tggtgcggcg agaacgtgtc gtagggcagg gtttcccagt cgggaaacag caccacccgc
    11521 aggtcggggg cgaaaaagcc gagctcgtcc agcagccgcc gcgcgtcgcc ggcgtcggcg
    11581 gtgacgatgg ccaccagccg ctgctcggcg gcctcgcgct gcgccagtcg cgccagcagc
    11641 agcgcgtcgc tggaggccgg cgggcggggc agggcatggc gtttgccagg gatgagcttg
    11701 ggcaggtgca tggcgggcag cggcggaaac gacaaaaacc cccggccggg tcgggcgggg
    11761 gcgtgacggg cctattttac cgctccggaa tctgaacgtt tttggactct ggcgcgctcc
    11821 agacaagcgc cagcagctat aaaaaagata gtagcgcagc ggccggcgcg gcttttcggt
    11881 ggccggtcta tagtggctgc ccgccaccgc aggagccgag cccatgagtc caaccgacga
    11941 tcgccccatc caaccgcagc gctacggcag cggccaggcc gtgcgccggc tggaggacga
    12001 cgcgctgctg cagggccagg gccgcttcac cgacgatctg ggcgcgccgg gcgacggctg
    12061 gttggtgttc gtgcgctcgc cgcacgcgca tgccgccctg cgcagcgtcg atgccgaggc
    12121 cgcgcgcgcc ctgcccgggg tgcgcgccgt catcaccggc gccgacctgg tggccgccgg
    12181 catgccgccg ctgccgactg gcgcgccctt caggcgcgcc gacggcagcg cctgcgccac
    12241 gccgccgcgg cgcgccctgg cgcacgaggt ggtgcgccat gtcggcgagg cggtggccgc
    12301 cgtggtggcc gacagcctgg cgcaggcgcg cgatgcggcc gacgcgctgg tggtcgacta
    12361 ccgcgagctg ccggtggtgg cctcgctggc ggcggccctg cgtgccgacg ccgtggtcac
    12421 gcccgccgcg cccgacaaca tcgcctgcga agcgcgccat ggcgatgcgg ccgccgcgca
    12481 ggcggcgttt gcgcaggcgg cgcacgtggt ggcgctcgat ctgacgcacc agcggctggc
    12541 cgcgctgacg ctggagccgc gcgcggtgcg cgccgcctgg gatgccgccc gcggccggct
    12601 ggacatgcac gccagcaccc agatgccgac ggcgctgcgc gccaccgtgg ccagcgcgct
    12661 cgggctgacg cccgaagccg tgcgcgtgcg cgtgggcgac gtcggcggcg gcttcggcat
    12721 gaagaccggc gcctaccccg aagacgtggt ggtggcctgg gccgcgcgcc acaccggccg
    12781 gccggtgcgc tggagcggcg agcgcggcga ggagtttctc agcaccaccc acggccgcga
    12841 cgtgcaggcg cacgccgagc tggcgctcga cgccgagggc cgcatgctgg cgctgcgcgt
    12901 gcactcgcac gccaacgtgg gcgcctacgc gctggccacc ggcgtggcga tccagctgct
    12961 gatcggcccc tgggtgcaga ccagcgtcta tcacgtgccg gtgatcgact ttcacttccg
    13021 cgccgtgctg acgcaccagg cgcccaccgg ggcctatcgc ggcgccggcc gccccgaggc
    13081 catcttcaac atcgagcgcc tgctggacga ggcggcgcgc cagagcggca tcgaccgcat
    13141 cgccctgcgg cggcgcaact tcatccgccc cgagcagatg ccctacacca acccgatggg
    13201 ccagacctac gacgtcggcc gcttcgagcg cgtgctggat caggcgctgc cgctggccga
    13261 ctgggacggc tttgcggcgc gcgcgcagca gtcgagggcg cgcgggcttt ggcgcgggct
    13321 gggcatcgcc acctttctgg agtggaccgg cggcaatgcg ttcgaggaga gcgtgaccct
    13381 ggaagtcaag tcggacggca tcgtcgaggt gttctccgcc gtcaaccaga tgggccaggg
    13441 cattgccacc tcgctggcgc agctggtggt cgatgtgctg caggtgccga tcgagcgcgt
    13501 gcgcgtggtg ctgggtgaca ccgagcgcgg caacggcttc ggcagcgccg gctcgcgctc
    13561 actgttcacc ggcggctcgg ccatgcgcgg cggcgctgag cacacgctgg cgcaggcgca
    13621 tcagctcgcg gccagcgcgc tggaagccgc gcccgaggac ctgcgctacc aggcggcgcg
    13681 ctttcacgtc gtcggtaccg accacgccat cggcctgttc gagctggccg gccgccagcc
    13741 gggcgcccac atccaggtcg agcacacgca caccgtggcc gggcccagct ggcccaacgg
    13801 cgcgcacatc tgcgaggtcg agctcgaccc gcagaccggc gccgtggccg tggtcggcta
    13861 caccagcgtc aacgacgtcg gccgcgtcgt caacccgctg atcgtgcgcg gccagctcga
    13921 cggcggcgcg gtgcagggca tcggtcaggc gctgtgtgag cagatcctgt acgacgccgc
    13981 cagcggccag ttgctcaccg gcagtctgat ggactacgcc gtgccgcgcg cgcacgagct
    14041 ggcgacgtgc tttcgcaccg agatggacga atccaccccc tgcgccaaca acgcgctggg
    14101 cgtcaagggc gtgggcgagc tgggcaccat cggcgccacg ccggcggtgg tcaacgccgt
    14161 ggccgatgcg ctggcgcgcg ccggccggcc cgaactggcg ccacgcctgc agatgccatt
    14221 gagcccggcg cggctgtggg cgctgctgca gtcgcagggg tagcgccgcg gcaaacatcc
    14281 gtacgccggg ggcgcagaag atttcaagat tttccgcatc ttccgcgtgt ctttggcgtt
    14341 cgttctgcaa ggaagcttgc agtcagcgcc ccacggaatc gatcaggatt tgtagcaata
    14401 tcggggctct ggcgaccacc catcaagcgc cgccagctat ccaagttgaa tttcctcatg
    14461 tcggttcaat ccccccgctg ccacgccctg gtgccctgcg ccggcagcgg cgcgcgcgcg
    14521 ggtggggtgc agcccaagca gtaccagctc gtggctggcc agccgctggt gcgccatacg
    14581 ctggcggcgc tggcgtccgt ggcgcgcttg gtccgcgtgc tggtggtggt ggcgccgggc
    14641 gacgatcggc tgcacgaact gccggcgccg ctggaggtgg cgccctgcgg cggcgccacg
    14701 cgggccgaga gcgtgctgaa cggcctgcgc catctgcgcg caactggcgc tgccgacagc
    14761 gactgggtgc tggtgcacga tgcggcgcgc tgcctgatca cgccggcgca ggtcgatgcg
    14821 ctgattgacg cctgctggca ggatgccgtg ggcggcctgc tggcgctgcc gctgcccgac
    14881 accctgaaga tggaagacgc gcagggccgc tcggccgcca ccgtcgagcg tgccggcaag
    14941 tggctggcgc agacgccgca gatgttccgc ctgggcgcgc tgcacgacgc gctggcggcg
    15001 cacgcggccc gcggctttgc cggtatcacc gacgaggcca gcgcgatcga ggccgccggc
    15061 ctgcggccgc tgctggtgtg cggcagcgcg cacaatgtca aggtgaccta ccccgaagac
    15121 tttgcgctgg ccgaagccgt gctgcgccac cgaacgccat gaccgatgcc gagccatcac
    15181 gcctgcccgc cctgcgcatc ggtgaaggct gggacgtaca cgccctggtg cctggccggc
    15241 cgctggtgat cggtggtgtg accgtcccgc acacacacgg gctgctgggc cattccgacg
    15301 ccgacgtgct gctgcacgcc gtcaccgacg cgctgctggg cgcggccggg ctgggcgaca
    15361 tcgggcggca ttttcccgat accgacgcgc gttttgccgg cgccgattcg gccgtgctgc
    15421 tggccgaggc ggcgcgccgc gtgggcgcgg cgggctggcg cgtgggcaac gtcgatggca
    15481 cggtgattgc ccaggcaccg aagctggcgc cgcacctgcc ggccatggtg gcgcgcatcg
    15541 cccaagtgct gggcgtgggc gtggctcagg tcaacgtcaa ggccaagacg gccgagcgcc
    15601 tggggccggt ggggcagggc gccagcatcg aggcgcgcgc cgtggtgctt ctatttcgat
    15661 agcttgtggc gcccgcccgg cgggcgctgc agccggaatt tccttgaacc tgcgggttcg
    15721 gaatcttgaa ctttgaaccg ttcaccctga gcctgtcgac gggctgcgac aagctcagcc
    15781 cgaacggtat tcaaggcatc ccaaggccga tccataggtt gaacctagga ggctgtcgga
    15841 cttggaaatc gccggctgca aatcgaccgc agcggcccat ttttggcttc gccg
//
LOCUS       DAOQTZ010000066        15127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4464_length_15127_cov_13.869029, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000066 DAOQTZ010000000
VERSION     DAOQTZ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 15127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15127
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4464_length_15127_cov_13.869029"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PKN51_08915"
     CDS             <1..854
                     /locus_tag="PKN51_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HNT85260.1"
                     /translation="RNEVPEIEQGLLEIKSCARDPGSRAKIAVVSHDKRVDPIGTCVG
                     VRGTRVNAVTNELAGERVDIVLWSDDPAQFVIGALAPANVSSIVVDEEKHAMDVVVDE
                     ENLAIAIGRGGQNVRLASELTGWKINIMDADESAQKEAEETGTLRTLFMEKLDVDQEI
                     ADILIEEGFTNLEEVAYVPIQEMLDIEAFDEDTVNELRARAKDALLTQAIAREESVES
                     VSQDLRELAGLTPELIALLADAGVKTRDDLADLAVDELTAITGQSEEDARALILKARE
                     HWFVDQE"
     gene            879..3683
                     /gene="infB"
                     /locus_tag="PKN51_08920"
     CDS             879..3683
                     /gene="infB"
                     /locus_tag="PKN51_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNT85261.1"
                     /translation="MTTITVSDFANELKRPADALLEQLQAAGVAKKSVSDALTDSDKQ
                     QLLNYLQASHGTGATRKKITLTKKTTSEIKQADATGKARTIQVEVRKKRTFIKRDEDA
                     APAPAPAVEPPAPAPAPAPAMPVVDAAEAARREEEARRQAELLRRQEEDAAERRRQRE
                     QAERETSEARAAAAEAPSIGAQAEAPAAAPAPAPAPAMPPAPAPTPAPAPAAAAAAEA
                     PSTAEDEAARAREQEESRRKALAEAEAIRAMMSAPRKVLVAKRAEDKKPEAKAAPQKA
                     GVAKPAAGPAAAPKEVKSAKLSSSWAGDPAKKKGIPTRGDTSAGRAGWRSGPRGGRRG
                     ERGREEQVVAAPVEQRVLEVHVPETITVAELAHKMAVKASEVIKTLMKMGQMVTINQP
                     LDQDTAMIVVEEMGHTAMVAALDDPEAFTDQEVQAQDAEALPRPPVVTVMGHVDHGKT
                     SLLDYIRRSKVAAGEAGGITQHIGAYHVETPRGVISFLDTPGHEAFTAMRARGAQATD
                     IVILVVAADDGVMPQTREAIKHAKAAEVPIVVAITKSDKPEANPERIKAELVGEEVVP
                     EEFGGESPFISVSSKTGQGMDELLENVLLQAEVLELKAPVDAAAKGLVIEASLDKGRG
                     PVATVLVQSGTLKVGDVVLAGQTSGRVRAMLDEDGRSTKTAGPSIPVEIQGLSEVPQA
                     GDDFMVLADERRAREIATYRAGKFRNTKLAKQQAAKLENVFADMQAGEVKTLPLIIKA
                     DVQGSQEALSASLLKLSTDEVKVQMVYSGVGGISESDINLAIASKAIVIGFNTRADAG
                     ARKLAEANDVQIRYYNIIYDAVDELKAAMSGMLAPEHKEEVIGMAEIRTVFVASKIGT
                     VAGSYVTSGVVRRDAHFRLLRDNVVIYTGELESLKRLKDDVREVKEGFECGIKLKNYN
                     DIKEGDQLEFFEIKEIARTL"
     gene            3820..4197
                     /gene="rbfA"
                     /locus_tag="PKN51_08925"
     CDS             3820..4197
                     /gene="rbfA"
                     /locus_tag="PKN51_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNT85262.1"
                     /translation="MPRRSSKPNRGYQIADQIQRDLAELIARELKDPRVGMVTLNAVE
                     VTPDYAHAKVFFSLLTGDPQETEEALNHAAGHLRNGLFKRLHIHTVPTLHFVFDRTTE
                     RAADMNALIARAVASRAKDEDPT"
     gene            4194..5147
                     /gene="truB"
                     /locus_tag="PKN51_08930"
     CDS             4194..5147
                     /gene="truB"
                     /locus_tag="PKN51_08930"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNT85263.1"
                     /translation="MTAAPRTRVQRRPVHGVLLLDKPLGLSSNQALQKVKWLLRAEKA
                     GHTGTLDPLASGVLPLCFGAATKFSALHLDADKRYHATLQLGVRTRTGDREGEVLACR
                     PVPPLSAALLEAVRQRFLGRILQLPPMHSALKKEGRALYEYARAGVEVERTPRAVRIH
                     ELVLTLAPAEQAPTAIEMIVFCSKGTYIRTLGEDIGEALGCGGHLSALRRVATGPFDQ
                     HQCVTLEQLEAMDEPARLACLRPPEVLLADHLPITLPAEEAGRFLSGMRRRGPWPDAD
                     RVAVFGEQPRALLGTAHVRAGELIPGRLLSPIELKQMLHLT"
     gene            5144..7396
                     /locus_tag="PKN51_08935"
     CDS             5144..7396
                     /locus_tag="PKN51_08935"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT85264.1"
                     /translation="MTTAPSSAPDTAAAALTRAAQAWQAGAWGQVIALLTPWVDATPP
                     APEALQLIAMAHLKLGQMAPALDRQRQLTRLQPGSAVVWINLAGTEAALGQTRQALAS
                     LDRALVLDPLQPAAHFNRANALMQLGEAAAACDGYRRAAELAPDRPDPPYNLAMALLQ
                     LDRHEEALGIMRAVVQRHPALAPAWNVLGMAWHRLNADQQALDSYQQAIRLDPGLADA
                     WSNGAQVLARLERRDEAQTWARQAVQLNPQHAAALRTLGVVLASAQAPQQKEARLWLE
                     KALELDPRDATALSTLVDLDTLHCDWSSLDAHLSRLRTAWHDSAPEGLELWRLLSLPI
                     PGHELRALTQATCARRWGKTAAQSAALPRRHVGQPRPQRLRIGYFSCDFRQHATSVLT
                     AGMFEAHDRSRFETVAYCLALNPPDASDPMRQRLRSAFERWESVGRLTDAELVRLARE
                     HDIHIAVDLQGHTAGARMGVFAARVAPIQLHYLAYAGTLGMPGSIDYLVTDPVVVPPA
                     ARPHFSEKLIELPDSYQVNDRARLIDPQRPSRAELGLPANGFVFCCFNNNYKIRRDVF
                     AIWMSVLRERPGSVLWLLADHAETVQNLRAAAEGHGIDPNRLVFAERTSLPRHLARQA
                     QADLFLDTWPYSAHTTASDALWAGLPLVTCAGETFASRVGASLLRACGLPQLIAHDPQ
                     AYREKILALSAAPEPLQAIRRQLLETRLSVPLFDTERFTRHIERAYDMAWERHMQGLA
                     PDHLTVPPLP"
     gene            7427..9253
                     /gene="typA"
                     /locus_tag="PKN51_08940"
     CDS             7427..9253
                     /gene="typA"
                     /locus_tag="PKN51_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNT85265.1"
                     /translation="MTRPIRNIAIIAHVDHGKTTMVDQLLRQSGTFAEHEKVVDTVMD
                     NNAIERERGITILAKNCAVSWQGTHINIVDTPGHADFGGEVERALSMVDGVLLLIDAQ
                     EGPMPQTRFVTKKALALGLKPIVVVNKVDKPGARPDHVINAAFDLFDKLGANDEQLDF
                     PVVYASGINGWSSLTEGEPGEQWGPDMAPLFETILKHVPPHQGDPNGPLQLQVSALDF
                     STFVGRIGVGRINAGTLRPGQDVLVMAGPEGASYKARVNEVLTFQGLDRIKVSEAGPG
                     DIVLVNGIEHIGIGETLTDPADPQPLPMLKIDEPTLTMNFCVNTSPLAGREGKFVTSR
                     QIWDRLQRELRSNVALRVKETSEDGVFEVSGRGELHLTILLEEMRREGYELAVSKPRV
                     VFKDIDGERCEPVEMVTADIEEGHQGGVMQALGERKGELVNMEPDGRGRVRLEYRIPA
                     RGLIGFTNEFLNLTRGSGLISNLFDGYEPHKGEIGGRKNGVLISMDDGEIFTYALGKL
                     DDRGRMFVRAGDPVYEGMIVGVHNRDNDLVVNATRTKQLTNFRVSGKEDAIKITPPIL
                     LTLEYGVEFIEDDELVEITPKSIRLRKRFLKEHERKRASREA"
     gene            9418..10380
                     /locus_tag="PKN51_08945"
     CDS             9418..10380
                     /locus_tag="PKN51_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT85266.1"
                     /translation="MASNLTAAAPPWSHGRAAWTMVLTTLLWSSAGVVTRQLQVAQGF
                     EVTFWRSFFTVLSLLLILPLWQGPGVFARMPWRRGTFWLSGLCWAVMFTAFMVALTLT
                     GVARVLVTMALGPLLTALLARVVTGHRLPPRTWAAIVLGGLGMAWMFAGQAGAADAPG
                     AWLGSLVALGVPAAAAVNWTVVQRSQAHGEPIDLVPSVLLGAVISSAITLPLAWPLTA
                     SPHDLGWLAFLGLTQLAIPCVLAVLCARVLPAAEVALLGLLEIVFGILLVWWIVGEAP
                     RPEVLSGGALVMGALLGNELLGWYAARRAAGAGAGSDIAPLGPA"
     gene            complement(10753..11232)
                     /gene="smpB"
                     /locus_tag="PKN51_08950"
     CDS             complement(10753..11232)
                     /gene="smpB"
                     /locus_tag="PKN51_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNT85267.1"
                     /translation="MATKKPKTSNPVIAENKKAAFNYFFEERFEAGMVLEGWEVKALR
                     AGKAQLTDGYVVIRAGELFVIGCQINPLGTASTHVRPDAVRTKKLLLHAEQIQRLIGK
                     VEQKGYTLVPLKLYWKNGRVKCEIALAKGKATHDKRDTIKEREGKREVERALKQRHR"
     gene            11483..11935
                     /locus_tag="PKN51_08955"
     CDS             11483..11935
                     /locus_tag="PKN51_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNT85268.1"
                     /translation="MKSVHKSVLIWYSAQEMFDLVVDVEHYPAFLPWCSHGKVMSHEE
                     GGMTAEVGIDFKGVRQTFVTRNTHVTGQEVHLHLVKGPFSRLEGRWVFTPVGQGGQRA
                     CRVDLRLNYGFSNAVLAKVVGPVFDRIASSLMDAFIKRAEQVYGAAAA"
     gene            11932..12303
                     /locus_tag="PKN51_08960"
     CDS             11932..12303
                     /locus_tag="PKN51_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNT85269.1"
                     /translation="MSPPPRPAACATLLEIELTYSPAPRQLVQRRLRLPGGSTVGDAL
                     KAAGLASPVTFAGGQVPWAVGVWGRPAALEQPLCAFDRVELWRPLRVDPKLARRERFG
                     QQGARAAGLFARRRPGAKPGY"
     gene            complement(12417..12950)
                     /locus_tag="PKN51_08965"
     CDS             complement(12417..12950)
                     /locus_tag="PKN51_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNT85270.1"
                     /translation="MRTSRILLIGLILAAAAAHAQYQWVDKDGRRVFSDRPPPADVPA
                     KNVLTQPKGARAPQATAAVGPSASASASEPASAAAGAAAARPAASAAGGVDKALEEKK
                     KQNEAAEAARKKAEEDRVAAARAENCKRARSAKATLESGMRMARVNDKGEREVLDDAQ
                     RATELQRVNAIIASDCK"
     gene            13362..>15127
                     /gene="thrS"
                     /locus_tag="PKN51_08970"
     CDS             13362..>15127
                     /gene="thrS"
                     /locus_tag="PKN51_08970"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNT85271.1"
                     /translation="MIHITLPDGSQREFPGPVTVAEVAQSIGAGLAKAALGGKVDGKL
                     VDTSFRIDHDVQLAIITAKDAQGLEMIRHSTAHLLAYAVKELFPEAQVTIGPVIENGF
                     YYDFSYKRPFTPEDLAAIEKKMEQLAAKDEPVLRRVLPRDEAVEYFKGLGEHYKAEII
                     ASIPANEDVSLYREGAFEDLCRGPHVPSTGKLRHFKLMKVAGAYWRGDHRNEMLQRIY
                     GTAWASKDDLKAYLTMLEEAEKRDHRRLGRELDLFHIDEHAPGTVFWHPKGWTVWQEV
                     EQYMRRVYRDNGYHEVKGPQILDKTLWEKTGHWDKYRENMFVTESEKRDYALKPMNCP
                     GHILIYKQGVKSYRDLPLRYGEFGNCHRNEPSGGLHGIMRVRGFTQDDGHIFCTEDMI
                     QAEVAAFTRLLQKVYRDFGFTEIIYKLSTRPAARIGTEESWDKAEAALAEGLRASGCE
                     FSYLPGEGAFYGPKIEYTLKDALGREWQCGTIQVDPNMPERLDAEYVGEDGARHRPIM
                     LHRAIVGSLERFIGILIEQHAGALPVWLAPVQVMVTGITDAQADYARSVAKTLRNQGF
                     RAETDLRNEKITYKIREHSLQKL"
BASE COUNT         2595 a         5039 c         5147 g         2346 t
ORIGIN      
        1 tccgcaacga ggtgcccgag atcgagcagg gcctgctgga gatcaagagc tgcgcgcgcg
       61 atccaggcag ccgcgccaag atcgccgtcg tcagccacga caagcgcgtg gacccgatcg
      121 gtacctgcgt cggagtgcgc ggcacgcgcg tcaacgccgt caccaacgag ttggcgggcg
      181 agcgcgtgga catcgtgctg tggtcggacg atccggcgca gttcgtgatc ggcgcgctgg
      241 cgccggccaa cgtgtcctcg atcgtggtcg acgaggaaaa gcacgccatg gatgtggtgg
      301 tcgacgagga gaacctggcc atcgccatcg gtcgtggcgg acagaacgtg cgcctggcct
      361 ccgagctgac gggctggaag atcaacatca tggacgccga cgagtcggcg cagaaggagg
      421 ccgaggaaac cggtacgctg cgcacgctgt tcatggaaaa gctcgacgtc gaccaggaga
      481 tcgccgacat cctgatcgag gaaggcttca ccaacctcga ggaagtggcc tacgtgccga
      541 tccaggagat gctggacatc gaggcgttcg atgaagacac cgtcaacgaa ctgcgcgccc
      601 gcgccaagga cgcgctgctg acccaggcga ttgcgcgcga agagagcgtg gaatcggtgt
      661 cgcaggatct gcgcgagctg gccggcctga cgcccgaact gattgccctg ctggccgacg
      721 ccggcgtgaa aacccgcgac gacctggccg atctggccgt agacgaattg accgcgatca
      781 cgggtcagtc cgaagaggac gcccgtgccc tgatcctgaa ggcgcgcgag cactggttcg
      841 tggatcaaga gtaaggatca tggaggcccg caccgcatat gacgaccatc accgtttccg
      901 atttcgcgaa cgagctcaag cgtccagctg acgcattgct ggaacagctc caggccgccg
      961 gggtggccaa gaagtcggtg tccgacgccc tgaccgacag cgacaagcag cagttgctga
     1021 actacctgca ggccagccat ggcaccgggg caacccgcaa gaagatcacg ctgaccaaga
     1081 agaccaccag cgagatcaag caggccgacg ccaccggcaa ggcacgcacc atccaggtcg
     1141 aggtgcgcaa gaagcgcacc ttcatcaagc gcgacgaaga cgccgcgccg gcgccagcac
     1201 cggcggtcga gcctccggcg cccgcgccgg cacctgcgcc cgccatgccg gtggtcgacg
     1261 ccgccgaggc cgcccggcgc gaagaagagg cacgccgcca ggccgagttg ctgcgccgcc
     1321 aggaagaaga cgcggccgaa cggcgccgcc agcgcgagca ggcggagcgc gaaacgtccg
     1381 aggcacgcgc agcggcggct gaagcgccgt cgatcggcgc acaggccgag gcgccggcag
     1441 ccgcgcctgc gcctgcgcct gccccggcca tgccgcccgc cccggcgccg acgcccgcac
     1501 ccgcacccgc tgcggctgcc gctgcggagg cgcccagcac tgccgaggac gaggccgcgc
     1561 gcgcccgcga acaggaagaa agccgccgca aggccctggc cgaggctgag gccattcgcg
     1621 ccatgatgag cgcgccgcgc aaggtgctgg tcgccaagcg ggccgaagac aagaaacccg
     1681 aggccaaggc ggcaccgcag aaagccgggg ttgccaagcc ggccgcaggg cctgcggccg
     1741 cgccgaagga ggtcaagtcg gccaagctgt cgtccagctg ggccggtgat ccggccaaga
     1801 agaagggcat tccgacccgg ggcgatacca gcgccgggcg tgccggctgg cgcagcggcc
     1861 cgcgcggcgg gcgccgtggc gagcgtgggc gcgaagagca ggtggttgcc gcaccggtgg
     1921 aacaacgggt gctggaagtg cacgtgcccg aaaccatcac cgtggccgag ctggcgcaca
     1981 agatggcggt caaggcctcc gaagtcatca agacgctgat gaagatgggc cagatggtca
     2041 ccatcaacca gccgctggac caggacacgg cgatgatcgt ggtcgaggaa atgggccaca
     2101 ccgccatggt ggcggcgctc gacgatccgg aggccttcac cgaccaggaa gtgcaggcgc
     2161 aggacgccga agcgctgccg cgcccgccgg tggtgaccgt gatgggtcac gtggaccacg
     2221 gcaagacctc gctgctggac tacatccgcc gcagcaaggt ggccgctggc gaagccggcg
     2281 gcatcacgca gcacatcggc gcctaccatg tcgagacccc gcgtggcgtg atttcgttcc
     2341 tcgacacgcc gggccacgag gcgttcaccg ccatgcgtgc gcgcggcgcg caggccaccg
     2401 acatcgtgat cctggtcgtc gcggccgacg acggcgtgat gccgcagacg cgcgaagcca
     2461 tcaagcacgc caaggcggcc gaagtgccga tcgtggtggc catcaccaag tccgacaagc
     2521 ccgaagccaa ccccgagcgg atcaaggccg agctggtggg cgaagaggtg gtgcccgagg
     2581 aattcggtgg tgagtcgccg ttcatctcgg tgtcgagcaa gaccggtcag ggcatggacg
     2641 agctgctgga gaacgtgctg ctgcaggccg aagtgctgga actgaaggcg ccggtcgatg
     2701 ccgccgccaa gggcctggtc atcgaagcca gcctggacaa gggccgcggc ccggtggcca
     2761 cggtgctggt gcagtcgggc acgctgaagg tgggcgacgt ggtgctggcc ggtcagacct
     2821 ccggccgcgt gcgcgccatg ctggacgagg acgggcgctc caccaagacg gccggcccgt
     2881 cgattccggt ggaaatccag ggcctgtccg aagtgccgca ggccggcgat gacttcatgg
     2941 tgctggccga cgagcggcgc gcgcgcgaaa tcgccaccta ccgcgccggc aagttccgca
     3001 acaccaagct ggccaagcaa caggcggcca agctggagaa cgtgttcgcc gacatgcagg
     3061 cgggcgaggt caagacgctg ccgctgatca tcaaggccga cgtgcaaggc tcgcaggagg
     3121 cgttgtcggc ttcgctgctc aagctcagca ccgacgaggt gaaggtgcag atggtctaca
     3181 gcggtgtggg cggcatcagc gagagcgaca tcaatctggc gatcgcctcc aaggccatcg
     3241 tgatcggctt caacacccgt gccgacgccg gggcgcgcaa gctggccgag gccaacgacg
     3301 tgcagatccg ctactacaac atcatctacg acgccgtgga cgagctgaag gcggccatga
     3361 gcggcatgct ggcgccggag cacaaggaag aagtcatcgg catggccgag atccgcaccg
     3421 tgttcgtggc cagcaagatc ggcaccgtgg cgggctccta cgtcaccagc ggcgtggtgc
     3481 ggcgcgacgc ccatttccgt ctgctgcgcg acaacgtggt gatctacacc ggcgagctgg
     3541 aatcgctcaa gcgcctgaag gacgacgtgc gtgaagtcaa ggaaggcttc gagtgcggca
     3601 tcaagctgaa gaattacaac gacatcaagg aaggcgacca gctggagttc ttcgagatca
     3661 aggagatcgc gcgtacgctg tgaggcttgt cggcccacaa tgaacagccg tacgcagaga
     3721 acgcagagat tgcgcaaagg gcgctgagaa agcaggacga tgccttctgg cgtcctctgc
     3781 gcttgctttg cgtcctctgc gtacggtggt tgaatttcca tgcccagacg cagcagcaaa
     3841 cccaatcgcg gctatcagat cgccgaccag attcagcgcg atctggccga gttgatcgcg
     3901 cgcgagctga aggacccgcg cgtgggcatg gtgacgctca atgccgtcga ggtcactccc
     3961 gactacgccc acgccaaggt gttcttcagt ctgctgacgg gcgacccgca ggaaaccgaa
     4021 gaggcgctca accacgccgc cggtcatctg cgcaacggct tgttcaagcg gctgcacatc
     4081 cacaccgtgc cgacgctgca cttcgtgttc gaccgcacca ccgagcgcgc ggccgacatg
     4141 aacgcgctca tcgcgcgggc ggtggcctcg cgcgccaagg acgaagaccc gacatgacgg
     4201 ctgccccacg caccagggtg cagcggcgcc ctgtgcatgg ggtgctgctg ctcgacaagc
     4261 cgctgggcct gtccagcaac caggcgctgc agaaggtcaa gtggttgctg cgcgccgaaa
     4321 aggccggtca caccggcacg ctcgatccgc tggccagcgg tgtgctgccc ctgtgctttg
     4381 gcgctgccac caagttcagc gcgctgcacc tggacgccga caagcgctac cacgccacct
     4441 tgcagctggg cgtgcgcacc cgcaccggag accgtgaggg cgaggtgctg gcgtgccggc
     4501 cggtgccgcc actgtcggcg gctttgcttg aagcggtgcg acagcgcttt ctggggcgca
     4561 tcctgcagct gccgcccatg cacagcgcgc tcaagaagga gggcagggcg ctgtacgaat
     4621 acgcgcgtgc cggcgtggag gtggagcgca caccgcgcgc ggtccggatc catgagctgg
     4681 ttctgaccct ggcgcccgcg gagcaagcgc cgacagctat cgaaatgata gtgttctgca
     4741 gcaagggcac ctatatccgc accctgggcg aagacatcgg cgaggccctg ggttgcggtg
     4801 gccacctgag cgcgttgcga cgggtggcga ccgggccgtt cgaccaacac cagtgcgtca
     4861 ccctggagca actggaagcg atggacgaac cggcgcggct ggcctgcctg cgcccccccg
     4921 aggtgctgct ggccgatcac ctgccgatca cgctgcccgc ggaggaggcg ggacgctttc
     4981 tgtccggcat gcgccgacgc ggcccctggc ccgatgccga tcgggtggcg gtgttcggcg
     5041 agcagccacg cgcgctgctg ggaaccgccc atgtgcgcgc cggcgagctg atccccggcc
     5101 ggctgctgtc gcccatcgag ttgaagcaga tgctccatct gacatgacca ccgcaccgtc
     5161 ctcagctccc gacacggccg ctgcggccct gacgcgcgcc gcacaggcct ggcaggcggg
     5221 tgcctggggc caggtgatcg cgctgctgac accgtgggtg gatgcgacgc cgcccgcgcc
     5281 cgaggcgttg cagctgatcg ccatggcgca cctgaaactg ggacaaatgg cgccggcgct
     5341 cgaccggcag cggcagctga cccggttgca gcccggcagc gccgttgtct ggatcaacct
     5401 ggcaggcacc gaggcggccc tcggtcagac ccggcaggcg ctcgcgagtc tggatcgcgc
     5461 gctggtcctc gatccgctgc agcccgcggc ccacttcaac cgcgccaatg cactgatgca
     5521 actgggcgaa gccgcagccg cctgcgacgg ctatcgccgt gccgccgagc tggcccccga
     5581 tcgtcccgac ccgccgtaca acctggccat ggccttgctg cagctggatc gtcacgaaga
     5641 ggcgctgggg atcatgcgtg ccgtggtgca acgccatccc gctctggccc ccgcgtggaa
     5701 cgtgctgggc atggcgtggc atcggttgaa cgccgatcag caggcgctgg acagctacca
     5761 gcaggccata cgcctcgatc ccgggctggc ggatgcctgg agcaacgggg ctcaggtgct
     5821 ggcgcggttg gagcggcgcg atgaagcgca gacctgggcg cgtcaggctg tgcagctgaa
     5881 cccccaacac gccgccgcac tgcgcacgct gggggtggtg ctggccagcg cccaggcgcc
     5941 ccagcagaag gaggcacgac tgtggctgga gaaggcgctt gagctcgatc cgcgcgacgc
     6001 cacggccctc agcactctgg tcgatctgga caccctgcac tgcgactgga gctcgctcga
     6061 cgcccacctg tcccgactgc gcaccgcgtg gcacgacagt gcgcccgagg gccttgagtt
     6121 gtggcgcctg ctgagtctgc cgatacccgg ccatgaactg cgcgcgctga cgcaagccac
     6181 ctgcgcgcgc cgctggggca aaaccgcagc ccagtcggcc gcgttgcctc gacggcatgt
     6241 gggccagccg cgtccgcagc ggctgcgcat cgggtacttt tcctgcgatt ttcgtcaaca
     6301 cgccaccagt gtgctgacgg ccggcatgtt cgaggcgcat gatcgctcgc gcttcgaaac
     6361 cgtggcgtac tgcctggcgc tgaacccgcc tgacgccagt gaccccatgc gtcagcgcct
     6421 gcggtcggcc ttcgaacggt gggagtcggt ggggcgtctg accgacgccg agctggtgcg
     6481 cctggcgcgt gagcacgaca ttcacatcgc cgtggacctg caaggccata ccgcgggcgc
     6541 gcgcatgggg gtttttgcag ctcgcgtggc gcccatccag ttgcactacc tggcctatgc
     6601 gggcaccctg ggcatgcccg gcagcatcga ctacctggtg accgacccgg tggtcgtgcc
     6661 gcccgccgct aggccgcact tcagcgagaa gctcatcgag ctgccggaca gctaccaggt
     6721 caacgatcgt gcgcgtctga tcgatcccca gcggccctcg cgcgccgagc tgggcctgcc
     6781 cgctaacggt ttcgtgttct gctgcttcaa caacaactac aagatccggc gcgatgtgtt
     6841 tgccatctgg atgtcggtgc tgcgcgagcg ccccggcagt gtgctgtggc tgctggccga
     6901 tcacgccgag acggtgcaga acctgcgggc ggcggccgaa ggccatggca tcgaccccaa
     6961 ccgattggtg tttgcggaac gcacgtcgtt gccgcggcat ctggcgcgcc aggcccaggc
     7021 cgacctgttc ctcgacacct ggccctacag cgcacacacc accgccagcg acgcgctctg
     7081 ggccgggctg ccgctggtca cctgcgccgg cgaaaccttt gcgtcgcgtg tgggcgccag
     7141 cctgctgcgc gcctgtgggc tgccgcagct gatcgcgcac gatccgcagg cgtaccgcga
     7201 aaagatcctg gcgctgtcgg ccgcgcctga gccactgcag gccatccgcc gtcagttgct
     7261 cgaaacccgg ctgtccgtgc ccctgttcga caccgagcgc ttcacgcgtc acatcgagag
     7321 ggcctacgac atggcgtggg agcgccacat gcagggattg gcccccgatc acctgaccgt
     7381 gccacctctg ccctgaccca tcgaccctga ctgatctgag ttcatcatga cccgacccat
     7441 tcgcaacatc gccatcattg cccacgtcga ccacggcaag accaccatgg tcgaccagct
     7501 gctgcgccag tccggcacct ttgccgagca tgaaaaggtg gtcgacaccg tgatggacaa
     7561 caacgccatc gaacgcgaac gcggcatcac catcctggcc aagaactgcg ccgtcagctg
     7621 gcagggcacc cacatcaaca tcgtcgacac gcccggccat gccgacttcg gcggtgaggt
     7681 ggagcgcgcg ctgtcgatgg tcgatggcgt gctgctgctg atcgacgcgc aggaaggccc
     7741 catgccgcag acgcgctttg tcaccaagaa ggcgctggcg ctgggcctca agcccatcgt
     7801 ggtggtcaac aaggtcgaca agcccggcgc gcggcctgac cacgtgatca acgccgcgtt
     7861 cgatctgttc gacaagctgg gtgcgaacga cgaacagctc gacttcccgg tcgtctacgc
     7921 ctccggcatc aatggctgga gctcgctcac cgagggcgag ccgggcgaac aatgggggcc
     7981 ggacatggcg ccgctgttcg agaccatcct caaacacgtg ccgccgcacc agggcgaccc
     8041 gaacggcccg ctgcagctgc aggtgtcggc gctggacttc tcgaccttcg tcggccgcat
     8101 cggcgtcgga cgcatcaacg ccggcacgct ccggcccggt caggacgtgc tggtaatggc
     8161 tggccccgaa ggcgccagct acaaggcgcg tgtcaatgaa gtgctgacct tccagggcct
     8221 ggatcgcatc aaggtcagcg aagccgggcc gggcgacatc gtgctggtca acggtatcga
     8281 gcacatcggc atcggcgaaa ccctgaccga tcccgccgac ccgcagccgc tgccgatgct
     8341 gaagatcgac gagcctacgc tgaccatgaa cttctgcgtc aacacctcgc cgctggccgg
     8401 gcgcgagggc aagttcgtca ccagtcgcca gatctgggac cggctgcagc gcgagctgcg
     8461 ctccaacgtc gcactgcggg tgaaggaaac cagcgaagac ggcgtgttcg aggtctccgg
     8521 ccgcggcgag ctgcacctga ccatcctgct ggaagaaatg cgccgcgaag gctacgaact
     8581 ggccgtatcc aagccgcgcg tggtgttcaa ggacatcgac ggcgagcgct gcgagccggt
     8641 ggagatggtc accgccgaca tcgaggaggg ccatcagggc ggcgtgatgc aggcgctggg
     8701 cgagcgcaag ggcgaactgg tgaacatgga gcccgacggc cgtggccgcg tgcgcctgga
     8761 ataccgcatt ccggcgcgcg gcctgatcgg tttcaccaac gagtttctga acctgacgcg
     8821 cggctcgggc ctgatcagca acctgttcga cggctatgag ccgcacaagg gcgagatcgg
     8881 cgggcgcaag aacggtgtgc tgatcagcat ggacgacggc gagatcttca cctacgccct
     8941 gggcaagctg gacgaccgcg gccgcatgtt cgtgcgcgcg ggcgatccgg tgtacgaggg
     9001 catgatcgtg ggcgtgcaca accgcgacaa cgacctggtg gtcaacgcca cgcgcaccaa
     9061 gcagctgacc aacttccgcg tcagcggcaa ggaagacgcg atcaagatca cgccgccgat
     9121 tctgctgacg ctggaatacg gggtcgaatt catcgaggac gatgagctgg tcgagatcac
     9181 gcccaagagc attcgcctgc gcaagcgctt cctgaaggag cacgaacgca agcgcgccag
     9241 ccgcgaggcc tgagcaagcc acaaccaacc cctcatgccc gctttgaacg caccgacaca
     9301 cccgctcggc gttgcccagg ctcgccgcag cccgagcgac ggctgcgcgg tgcaccctga
     9361 acgcccgcgt gggggtatcc cccgcgggcg atcggcattc attcccgtcg tccgaccatg
     9421 gcgtccaacc tgaccgctgc cgcgccgccc tggagccatg gccgcgccgc ctggaccatg
     9481 gtgctgacca cgctgctgtg gtccagcgcc ggcgtggtga cgcgccagtt gcaggtggcg
     9541 caaggcttcg aggtgacgtt ctggcgcagc ttcttcaccg tgctgtcgct gctgctgatc
     9601 ctgccgctgt ggcaggggcc gggcgtgttc gctcgcatgc cgtggcggcg cggcacgttc
     9661 tggttgtcgg gcctgtgctg ggcggtcatg ttcaccgcct tcatggtggc gctgaccctg
     9721 accggggtgg cgcgcgtgct ggtcaccatg gcgctggggc cgttgctgac ggccttgctg
     9781 gcgcgcgtgg tcacgggcca ccgcctgccg ccgcgcacct gggcggccat cgtgctgggc
     9841 ggcctgggca tggcctggat gttcgccggt caggcgggcg cggcggacgc ccccggggcg
     9901 tggctggggt cgctcgtggc gctgggcgtg ccggcggccg cggccgtcaa ctggacggtg
     9961 gtgcagcgca gccaggcgca tggcgagccg atcgatctgg tgccgtcggt cttgctgggc
    10021 gcggtgatct ccagcgccat caccttgccg ctggcctggc cgctgacggc gtcgccgcac
    10081 gacctgggct ggctggcctt cctgggattg acgcagttgg ccatcccctg cgtgctcgcg
    10141 gtgctgtgcg cccgtgtgct gccggcggcc gaggtggcgc tgctgggtct gctggagatc
    10201 gtgttcggca tcctcctggt gtggtggatc gtaggcgaag cgccgcgccc cgaggtgctc
    10261 agcggtggtg ccctggtcat gggcgccctg ctgggcaatg aactgctcgg ctggtacgcc
    10321 gcacggcgcg ccgcaggggc gggcgccggc agcgacatcg cgcccctcgg gccggcctga
    10381 cgccagatcg tacgctctga ccactcaatc ggccgacagc gcttgttggt aaagcgcaaa
    10441 cagctacata aacgatagca taccaaccta tcagaccggc cagcgcttca gcccaagaac
    10501 ggccgttctg ggttcaacct agaaacggca gttggacgcc cccgcccgtg ccgtgatcgg
    10561 cgtgccaggc aagacgcgac gacgccgcgg gctcctgccc gcaaggagga gcaacgccgc
    10621 atggcgcgcc gagcgcggtg cgggtgggtg cgtagcgtgc tcacccagga ggtgaaggcc
    10681 atcgcagccg cgagtccccg catcgatgcg gcctgcccga agttgacaag cggtcaactg
    10741 ccgtttccag gttcaacggt ggcgctgctt gagggcgcgc tcaacctcgc gcttaccctc
    10801 gcgttccttg atggtgtcgc gcttgtcgtg cgtggccttg cccttggcca gggcgatttc
    10861 gcacttcacg cgcccgttct tccagtacag cttgagcggc accagggtgt agcccttctg
    10921 ctccaccttg ccgatcaggc gctggatctg ctcggcgtgc agcagcagct tcttggtgcg
    10981 tacggcgtcg ggccgcacat gggtgctggc cgtgcccagc gggttgatct ggcagccaat
    11041 cacgaacagc tcgccagcgc ggatcaccac gtagccgtcg gtcagctgcg ccttgcccgc
    11101 gcgcagcgcc ttgacttccc agccttccag caccatgccg gcttcgaagc gctcttcgaa
    11161 gaagtagttg aacgcggctt tcttgttttc ggcgatgacc gggttgctgg tcttgggttt
    11221 cttggtggcc atcgatgcaa tatggtgggt tgctcgcact ttgcaagcgc atgcgcgagt
    11281 gtggcggcgc ttgaggcaat gctgaacgcg tcccaggcgc gcgtcgcctg tcggtcagtg
    11341 gcggtctgcg gccctgccga tgttcgccat ggcctcgtcc atggcggtgc cttgcgccct
    11401 gccgcccagc ccgactcagg gcgcgcggca tcgcgggaac cggttcagcg ccgccctaaa
    11461 atcggcgaca ggcagcgatt ctatgaaatc cgtccacaaa tccgtcctga tctggtacag
    11521 cgcacaggag atgttcgatc tggtggtcga cgtcgagcac tatccggcct ttctgccctg
    11581 gtgcagccat ggcaaggtga tgtcgcatga agagggcgga atgaccgccg aggtgggcat
    11641 cgacttcaag ggcgtgcgcc agaccttcgt cacccgcaac acccacgtca cggggcagga
    11701 agtgcacttg cacctggtca aggggccgtt ttcgcggctg gaagggcgct gggtcttcac
    11761 gccggtgggg cagggcggtc agcgcgcctg ccgcgtggac ctccgactga actacgggtt
    11821 ttccaacgcc gtactggcca aggtggtcgg cccggtgttc gaccggatcg ccagcagcct
    11881 gatggatgcc ttcatcaagc gcgccgaaca ggtctacggc gccgccgctg catgagcccg
    11941 ccaccgcgcc cggcggcctg cgcgacgctg ctggagatcg agctgaccta ttcgcccgct
    12001 ccgcgccagc tcgtgcagcg ccggctgcgc ctgcccggcg gcagcactgt gggcgacgcg
    12061 ctgaaggccg ccggcctggc tagcccggtc acgttcgccg gcggccaggt gccgtgggcg
    12121 gtgggggtgt ggggccgacc ggccgcgctt gagcagccgc tgtgtgcgtt cgatcgcgtg
    12181 gagttgtggc gccccttgcg cgtcgatccc aagctggcgc ggcgcgagcg gttcggccag
    12241 cagggcgccc gcgccgcagg cctgttcgcc aggcgacgcc cgggcgccaa gccgggctat
    12301 tgacccctct tgccctgatg ggctgccagc acaccacggt tgagttcgct gtccaccggc
    12361 aatgctacta attcagtagc tgccggcgct tgcccacaaa gggccgacag cacatatcac
    12421 ttgcagtcgc tggcgatgat ggcgttgacc cgctggagtt ccgtcgcgcg ctgggcgtcg
    12481 tcgagcacct cgcgctcgcc cttgtcgttg acgcgcgcca tgcgcatgcc ggattccagc
    12541 gtggccttgg cgctgcgggc gcgtttgcag ttttctgccc gagcggccgc cacgcgatct
    12601 tcctcggctt tcttccgggc ggcctcggcc gcctcgttct gttttttctt ctcttccagc
    12661 gccttgtcca cgccgcccgc ggcggacgcc gcggggcgtg cggcagcggc gcctgctgcc
    12721 gcgctggcag gctcgctggc agaggcagag gcagaggggc cgaccgcggc ggtcgcctgc
    12781 ggtgcgcgcg cgccctttgg ttgcgtcagc acgttcttgg caggcacgtc ggctgggggc
    12841 ggacggtcgc tgaacacgcg gcgcccgtcc ttgtccaccc actggtactg ggcgtgggcg
    12901 gctgcggcgg cgaggatgag tccgatgagc agaatgcggc tggttctcat aaacctgcag
    12961 tgtaaccgtg cgtgacactt atggctgtat cgcgcgcccg acagcaggcg tggggcttat
    13021 agccctgaaa tgtgcccagg gtgcgctgtt tctggctccc atcggccttg gtaccgctct
    13081 gctcgaaaat ggccaattgt ggactggcgg cgcttctgct ggcggcgctt ctgcggggct
    13141 tcggataaaa tatgagcttt ggcgggttgc ggcgcagccc ccacggtgca gattcacaag
    13201 gacgagaaag gcagcatcgg ttatgacacc gcgaactacc accgagggtt tcaccaccgt
    13261 cgtctgacgg tgcggggttc gcgcctgttc ctttcgtcct ttcccagatc aagcgcggcc
    13321 cggtccgcgc ttttttgttt ttccacgcaa ggtttccccc catgattcac atcacgcttc
    13381 ccgacggctc ccagcgcgag ttccccggcc cggtgacggt ggccgaggtg gcgcagtcca
    13441 ttggcgccgg tctggccaag gcggccctgg gcggcaaggt ggacggcaaa ctggtcgaca
    13501 ccagcttccg catcgatcac gacgtgcagc tggccatcat caccgccaag gacgcgcaag
    13561 ggctggagat gatccgccac tccacggccc acctgctggc ctatgcggtc aaggagctgt
    13621 ttccggaggc gcaggtcacc atcggcccag tgatcgagaa cggtttctac tacgacttca
    13681 gctacaagcg acccttcacg cccgaggacc tggcggcgat cgagaagaag atggaacagc
    13741 tggccgccaa ggacgagccg gtgctgcgcc gcgtgctgcc gcgcgacgag gcggtcgaat
    13801 acttcaaagg tctgggcgag cattacaagg ccgagatcat cgccagcatc cccgccaatg
    13861 aagacgtcag tctgtaccgt gaaggcgcct tcgaggacct gtgccgcggc ccccacgtgc
    13921 ccagcacggg caagctgcgg catttcaagc tgatgaaggt ggcgggcgcc tactggcgcg
    13981 gcgaccaccg taacgagatg ctgcagcgca tctacggcac ggcctgggcc agcaaggatg
    14041 atctcaaggc ctacctgacc atgctggagg aggccgaaaa gcgcgaccac cgccgtctgg
    14101 ggcgcgagct cgacttgttt cacatcgacg agcacgcccc cggcacggtg ttctggcacc
    14161 ccaagggctg gaccgtgtgg caggaggtcg agcagtacat gcgccgcgtc taccgcgaca
    14221 acggctacca cgaagtcaag ggcccgcaga tcctggacaa gacgctgtgg gagaagaccg
    14281 gccactggga caagtaccgc gagaacatgt tcgtcacgga atcggaaaag cgcgactacg
    14341 cgctcaagcc gatgaattgc cccggccaca tcctgatcta caagcagggc gtcaagagct
    14401 accgcgacct gccactgcgc tacggggaat tcggcaactg ccaccgcaac gaaccgtcgg
    14461 gtggcctgca cggcatcatg cgggtgcgcg gcttcacgca ggatgacggg cacatcttct
    14521 gcaccgaaga catgatccag gccgaagtcg cggccttcac gcggttgctg cagaaggtct
    14581 acagggactt cggtttcacc gagatcatct acaagctgtc cacgcgcccg gccgcgcgca
    14641 tcggcaccga agagagctgg gacaaggccg aggccgcgct ggccgagggt ctgcgcgcgt
    14701 cgggctgcga gttcagttac ctgccgggcg agggcgcgtt ctatggcccc aagatcgagt
    14761 acacgctgaa agacgcgctg ggccgcgaat ggcagtgcgg cacgatccag gtggacccga
    14821 acatgcccga gcggctggac gccgaatacg tgggcgagga cggcgcgcgt caccgcccca
    14881 tcatgctgca ccgtgccatc gtcggcagcc tggagcgctt catcggcatc ctgatcgagc
    14941 agcacgccgg tgcgctgccg gtgtggctgg cgccggtgca ggtgatggtg accggcatca
    15001 ccgacgcgca ggccgactac gcccggagcg tggcgaaaac gctgcgaaat caagggttta
    15061 gggccgaaac cgacctgcgc aacgagaaga tcacgtataa aatacgcgag cattcgttgc
    15121 aaaagct
//
LOCUS       DAOQTZ010000067        15041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4488_length_15041_cov_13.856866, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000067 DAOQTZ010000000
VERSION     DAOQTZ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4488_length_15041_cov_13.856866"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PKN51_08975"
     CDS             complement(<1..997)
                     /locus_tag="PKN51_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNT85272.1"
                     /translation="MADPILLAQHGDIQCVLLPGLANRHGLITGATGTGKTVTLQKLA
                     ESFSAIGVPVFMADVKGDLTGISQAGRIEGKLAAVIQERGLPQPEPTACPVTLWDVFG
                     QQGHPVRATVSDMGPLLLARMLDLNETQAGVLNLVFKIADDHGLLLLDLKDLRAMLQY
                     VGDNARQFTTDYGNVSAASIGAIQRGLLQLESQGGTQFFGEPMLNLEDMMQTVAGKGV
                     INILSADRLLQSPRLYATFLLWLLSDLYERLPEIGDPDKPKFVFFFDEAHLLFNDAPK
                     VLLERIELVVRLVRSKGVGVYFVTQNPIDVPDTVLAQLGNRVQHALRAFTPRDQKA"
     gene            1064..1792
                     /locus_tag="PKN51_08980"
     CDS             1064..1792
                     /locus_tag="PKN51_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT85273.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRQRIWTRVVREIMVAARSGGGDPS
                     ANPRLRLAIDKAKAANMPADTIKRNIDKATGNLEGASYEEIRYEGYGIGGAAIIVDTM
                     TDNRVRTVAEVRHAFSKYGGNLGTEGSVAFQFRHVGQFFFAPGTSEDQVMEVALEAGA
                     DDVITDDDGAIEVLCDPGAFEAVKQALEAAGLTPEMAEVTLRADSSIDLAGDDAARMQ
                     KLLDVLEDLDDVQAVYHNASLELA"
     gene            1800..1967
                     /locus_tag="PKN51_08985"
     CDS             1800..1967
                     /locus_tag="PKN51_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85274.1"
                     /translation="MRTPLAVLLLAAALAGCGGGELSWCIHSGSGSLAGGPNPPDCPP
                     DPKPDRSLQAS"
     gene            2002..3288
                     /gene="purD"
                     /locus_tag="PKN51_08990"
     CDS             2002..3288
                     /gene="purD"
                     /locus_tag="PKN51_08990"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNT85275.1"
                     /translation="MKILVIGSGGREHALAWKLSTSPRVQQVYVAPGNGGTALDPVLK
                     NVPISDVAALRDWALAEKIALTVVGPEQPLAAGVVDDFRAHGLRIFGPTRAAAQLESS
                     KAFSKAFMQRHGIPTAAFQTFTDAAAARAYVERMGAPIVVKADGLAAGKGVVVAQTVA
                     EAHAAIDDMLGGNSLGVAHNEGGARVVIEEFLPGEEASFIVLSDGKSVLALASSQDHK
                     RLQDGDQGPNTGGMGAYSPAPVVSADVHARVMREVILPTVRGMEQDGIPFTGFLYAGL
                     MIDAQGRPRTLEFNTRMGDPETQPILMRLKSDLVEVLLAATAGRLHEVELQWDRRVAL
                     GVVLAAAGYPLAPRKGDPITGLPAPQEDVRVFHAGTVADGGQVRTAGGRVLCVTALAD
                     SVRQAQARAYDAVRGIHFEGMQYRHDIGHHAIRQNP"
     gene            3290..4207
                     /gene="hemF"
                     /locus_tag="PKN51_08995"
     CDS             3290..4207
                     /gene="hemF"
                     /locus_tag="PKN51_08995"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNT85276.1"
                     /translation="MSAFSSRVHDYLSGLQSRITQAIEAADGRAVFRRDDWHKAAGEP
                     LQGAGSTRILEGGAVFERAGIGLSQVRGPRLPPSATQHRPELEGSPFEAMGVSLVFHP
                     LNPYVPTVHMNVRMISAGHPGAEPVCWFGGGMDLTPYYGFDEDCQHFHRTCRDALAPF
                     GDDTYPRFKAWCDDYFFNRHRGEARGIGGIFYDDFVGGGAEQGLALTRAVGEAFLQAY
                     LPIVERRAATPWGERERQHQLHRRGRYVEFNLVWDRGTHFGLQSGGRTESILLSMPPL
                     VAWQYQRQPEPGSPEARLTEHFLVPRAWV"
     gene            4362..4991
                     /gene="nadD"
                     /locus_tag="PKN51_09000"
     CDS             4362..4991
                     /gene="nadD"
                     /locus_tag="PKN51_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNT85277.1"
                     /translation="MTAGIHRVGLFGGAFDPPHHAHVALAQAAIEQLGLERLHVVPTG
                     DAWHKARALSPAADRLAMCRLAFAGLPRVAVDERELRRAGASYTIDTLTELHAEHPAA
                     QWFLLIGADQAAAFHSWRRADDILRLATIVIAVRADPSGVGAVFDAEHPLPGLAVAAD
                     RVRRLQLPAMPHSATAVRRLAAAGQPIEHLVPAPVAGYIADHHLYQSPA"
     gene            4991..5629
                     /gene="rsfS"
                     /locus_tag="PKN51_09005"
     CDS             4991..5629
                     /gene="rsfS"
                     /locus_tag="PKN51_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNT85278.1"
                     /translation="MSETAAKKDLQKLQRAIVDGLEDIKAQDIRVFNTEHLSPLFERV
                     IVATGSSNRQTKALATSVRDKVREAGFAKPRMEGEDNAEWIIVDCGQAVAHVMQPAIR
                     QYYKLEEIWGEKPVRLRNAAPLSRSSAAAPAAKKAAAAAPAAKKAATAARKTATAGTA
                     AKKAPAGKAPARKAAATPAAPAKRAPAKKAVAGKTAAVKSAAGKPAPRRKPA"
     gene            5975..6445
                     /gene="rlmH"
                     /locus_tag="PKN51_09010"
     CDS             5975..6445
                     /gene="rlmH"
                     /locus_tag="PKN51_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNT85279.1"
                     /translation="MKLIVIAVGQRLPGWAQTAWDDYAKRFPPELRLQLKAVKTEPRA
                     GGRTAPQAMAAERQRIEAALPRGAHVVVLDERGSALTTVALADRLRQWQGQGGDVALL
                     IGGPDGLDPALRQAAHERIRLSDLTLPHALVRVLLVEQLYRAWSVNAGHPYHRE"
     gene            6488..7081
                     /locus_tag="PKN51_09015"
     CDS             6488..7081
                     /locus_tag="PKN51_09015"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNT85280.1"
                     /translation="MSFIYLASQSPRRRQLLDQLGVAHRLLLPDDAAAAEALEAVRPG
                     EAPAAYVRRVTRLKLDAALRRLHQQGLPPAPVLCADTTVALGRHILGKPADAREAAAM
                     LRQLAGGTHRVITALALQAGERRHEALSDSRVRFAPVSETELADYLASGEWQGKAGAY
                     AIQGRAAAFIAHISGSYSGIMGLPLFETHQIMRLARL"
     gene            7104..8594
                     /gene="rng"
                     /locus_tag="PKN51_09020"
     CDS             7104..8594
                     /gene="rng"
                     /locus_tag="PKN51_09020"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HNT85281.1"
                     /translation="MQQDILINWSPQETRVAVVESGAVQELHVERALERGLVGNIYLG
                     KVARVLPGMQSAFIDIGLERAAFLHVADLWQKPANGDGLPAHRTGAPTRPIEKQVFEG
                     QTLMVQVIKDPIGSKGARLSTQVSLAGRMLVFLPQDDHVGVSQKIPAAQRDELRGRVQ
                     ALVAAGGEGGRSGGFILRTNAEDASDAELAEDIAYLRKTWARIRESATRQPPATLLHE
                     DLSLLQRVLRDLTSEATQSIRIDSREQFDGLLAFGQEFMPAAAARLSHYKGERPIFDL
                     FNVDEEITKALGRRVDLKSGGYLIVDQTEALTTIDVNTGGFVGARNFDDTVFRTNLEA
                     ALAIARQLRLRNLGGIIIIDFIDMTQPEHQQAVLAELRKQLGRDRVKTQVGGFSPLGL
                     VEMTRKRTRESLAQLLSEPCTQCQGSGRVRTARTVAYDILREILREARQFNPKEFRIV
                     AAPAVVEMLLDEESQHLAGLSDFIGKPISLQAESSIGPDQYDIVLL"
     gene            complement(8649..11846)
                     /gene="ppc"
                     /locus_tag="PKN51_09025"
     CDS             complement(8649..11846)
                     /gene="ppc"
                     /locus_tag="PKN51_09025"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNT85282.1"
                     /translation="MRTTRTAPAAIPTASPARRPSRSDKELPLIQDIRLLGRLLGDVI
                     REHEGTATYELVERIRTLSVAFRRDADQRAGNTLRRLLNGLSGEQTVSVIRAFTCFSL
                     LANLAEDRHHIRRRAVHERAGSAPEGGIEVALQRLHAAGIDAESIARTLSDSYVAPVL
                     TAHPTEVQRKSILDAEREIAQLLAQRDELRERALCQPGGRDVLSPRQLAANETRLHAR
                     VVLLWQTRLLRYDRLSVADEIENALSYYESTFLREIPRLYADLEQALGAARPVASFLR
                     MGQWIGGDRDGNPNVSAQTLHLALRRQAEAALLHYLDEVHELGRTLSLSSRLTAVSPA
                     LAALAQRSPDTSTHRSDEPYRRALTGIYARLSATLERLAGRGAPRPPAVQLAAYARAD
                     ELLAELRTIEGSLRAHDGQALVADRLQPLMRAVEVFGFHLATVDLRQSSDQHERVVAE
                     LLARAQLAPDYASLDEHARCALLLRLLNDARPLRVVGGQYSAHTQGELAIFEAALALR
                     HSHGHDAIRHYIISHTETVSDLLEVLLLQKEVGLMRGTLGGEATADLIVVPLFETIAD
                     LRHAAPIMRAFYAIPGIAALVRASGAEQDIMLGYSDSNKDGGIFTSNWELYRAEIALV
                     ALFDELNREHAEAPIRLRMFHGRGGTVGRGGGPSYQAIMAQPPGTVRGQIRLTEQGEV
                     IASKYANPEIGRRNLETLVAATLEATLLQPTQAAPDEFLQAAEQLSRASMAAYRALVY
                     DTPGFADYFFTATPIREIAQLNIGSRPASRKASDRIEDLRAIPWGFSWGQCRLTLPGW
                     YGFGAAVQTFMDDPAAPPRARLALLRRMHREWPFFRTLLSNLDMVLAKSDLALGSRYS
                     ELVADRRLRRRVFGAIEAEWQRTNASRKASDRIEDLRAIPWGFSWGQCRLTLPGWYGF
                     GAAVQTFMDDPAAPPRARLALLRRMHREWPFFRTLLSNLDMVLAKSDLALGSRYSELV
                     ADRRLRRRVFGAIEAEWQRTNDALTQITGSRQRLAGNAALARSIRHRFPYIDPLHHLQ
                     VELLRRWRAQPGDERVQTGIHLCINGIAAGLRNTG"
     gene            complement(11896..13551)
                     /locus_tag="PKN51_09030"
     CDS             complement(11896..13551)
                     /locus_tag="PKN51_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HNT85283.1"
                     /translation="MNGAQALIKTLADAGVEVCFSNPGTSEMHFVAALDSEPRMRAVL
                     ALFEGVATGAADGYARMAGKPAAVLLHLGCGLGNGLANLHNARKGKVPVVNIVGDHAT
                     YHTQYDAQLQSDIETVARNVSPGFVRTSKSTAQLGEDAVDAIVAARGLPGQVATLILP
                     ADVSWGEGGQPCAPPPALKPQAADDATVQTIAHAIRSGKKTALLLGGQALREPALLAA
                     ARIAAHSGVQLLAECFPTRMERGAGLPFVERIAYLAEMAGVQLAPIEQLILVDAKAPV
                     SFFAYPGKRSYLVPETCQVHTLATPEQNAAASLEQLAAALGAGAAQPRLQAPQRPNRP
                     TGRLTAPKVCKAVGALLPERAIVIDEAITSGLMLGVMTAGCPRHDLITLTGGAIGQGL
                     PNAVGAAIACPQRPVIALIGDGTAMYTIQALWTMARENLNVTAIIFNNASYSVLNVEL
                     DRVGADEAGPKAKSQLDLHGPVLSFAQLAQGMGVHAARTHTAEDFCQALEYALGQSGP
                     HLIEAMVPESLGGAKRKVLPWLLKSLPSLPQPVAQALKKKIAP"
     gene            complement(13621..>15041)
                     /locus_tag="PKN51_09035"
     CDS             complement(13621..>15041)
                     /locus_tag="PKN51_09035"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HNT85284.1"
                     /translation="ANLRDCFAAQQAAFAQNRYPSLQERRADLRALHRLLVENRDALV
                     DAVNRDFGCRSHFETQFAELLQGQEGALDAIRRLPRWMKKQSRHLDMTQYPLASAFVQ
                     PQPLGVVGIVAPWNFPIAMVVQPLIGALAAGNRAMIKMSENSERLARLLQELLPKYFA
                     ADKVSLFADSDAGPGQSLGPLFTQLPFNHLFFTGSPQTGKAVMANCAANLTPVTLELG
                     GKSPAIVAPGYAMHKAAERILWVKMLNAGQICTNVDYMFVPAGQEQAFIEAAQRIVAQ
                     RYPDLTSGDYTAIIDQRQYDRLQAMLADAVAQGAQVVPLAPGQQGDAARRIMPPVALL
                     GVHDGMQVMQREIFGPLLPILSYQTHDEVLRYINDRPHPLALYLFSDDKALQQRYLQQ
                     TLSGGVTLNDTLLHVGQHSLPFGGVGNSGMGHYHGHEGFLTFSKLRPVFRQGPLRTVD
                     FLMPPYRGMARRMLDVMFWRNR"
BASE COUNT         2284 a         5123 c         5388 g         2246 t
ORIGIN      
        1 ccgccttctg gtcgcgcggg gtgaaggcgc gcagcgcgtg ctgcacgcgg ttgcccagct
       61 gcgccagcac ggtgtcgggc acgtcgatcg ggttctgcgt cacgaagtac acgcccacgc
      121 ccttggagcg caccagccgc accaccagct cgatgcgctc gagcagcacc ttgggcgcgt
      181 cgttgaacag caggtgcgcc tcgtcgaaga agaacacgaa cttgggcttg tccgggtcgc
      241 cgatttccgg caggcgctcg tacaggtcgc tcagcagcca cagcaggaag gtggcataca
      301 ggcgcggcga ctgcagcagc cggtcggccg acaggatgtt gatcaccccc ttgccggcga
      361 cggtctgcat catgtcttcg aggttcagca tcggctcgcc gaagaactgc gtgccgccct
      421 ggctttccag ctgcagcagg ccgcgctgaa tggcgccgat gctggcggcg ctgacgttgc
      481 cgtaatcggt ggtgaactgc ctggcgttgt cgcccacgta ctgcagcatg gcgcgcaggt
      541 ccttcaggtc cagcagcagc aggccgtggt cgtcggcgat cttgaacacc aggttcagca
      601 cgccggcctg ggtttcgttc agatccagca tgcgcgccag cagcagcggc cccatgtcgc
      661 tgacggtggc gcgcaccggg tggccctgct ggccgaacac gtcccacagc gtgaccgggc
      721 aggcggtcgg ctcgggttgc ggcaggccgc gctcctggat gaccgcggcc agcttgccct
      781 cgatgcggcc ggcctggctg atgccggtca ggtcgccctt gacatccgcc atgaacaccg
      841 gcacgccgat ggccgagaac gattcggcca gcttctgcag cgtcaccgtc ttgccggtgc
      901 cggtggcgcc ggtgatcagg ccgtggcggt tggccaggcc gggcaaaagc acgcactgaa
      961 tgtcgccgtg ctgggcaagc agaatcggat cggccatggg tcaaggtccg tgaaaagtaa
     1021 aatcgccaca ttatcaagcg actttcgaga gggtttccgc gtcatggccg gccacagcaa
     1081 atgggcaaac attcaacacc gcaagggacg ccaggacgaa aagcgccagc gcatctggac
     1141 ccgcgtggtg cgcgaaatca tggtggccgc gcgcagcggc ggcggtgacc cgtcggccaa
     1201 cccgcgcctg cggctggcca tcgacaaggc caaggccgcc aacatgccgg ccgacaccat
     1261 caagcgcaac atcgacaagg ccaccggcaa cctcgaaggc gcaagctacg aggaaatccg
     1321 ctacgagggc tacggcatcg gcggtgccgc catcatcgtc gacaccatga ccgacaaccg
     1381 cgtgcgcacc gtggccgaag tgcgccacgc cttcagcaag tacggcggca acctgggcac
     1441 cgaaggttcg gtggcgttcc agttccgtca tgtggggcag ttcttcttcg cccccggcac
     1501 cagcgaggac caggtgatgg aagtcgccct ggaggccggc gccgacgacg tgatcaccga
     1561 cgacgacggc gccatcgagg tgctgtgcga ccccggcgcc ttcgaggccg tcaagcaggc
     1621 gctggaggcc gccggcctga cgcccgagat ggccgaggtg acgctgcgcg ccgacagcag
     1681 catcgatctc gccggcgacg acgcggcccg catgcagaag ctgctcgacg tgctggaaga
     1741 cctggacgat gtgcaggcgg tctaccacaa cgccagcctg gagctggcct gaacgggcga
     1801 tgcgcacgcc gctcgccgtg ctgctgctgg ccgccgcgct ggcgggctgc ggcggcggcg
     1861 agctgtcgtg gtgcatccac agcggctcgg gttcgctggc gggcggcccc aaccctcccg
     1921 actgcccgcc cgatcccaag ccggatcggt cattgcaggc gtcctgactg cgcaagcagc
     1981 tattattttt agagtgattc gatgaagatc ctggtcatcg gctcgggcgg ccgcgagcac
     2041 gccctggcct ggaagctcag cacctcgccg cgcgtgcagc aggtctatgt ggcgcccggc
     2101 aacggcggca cggcgctgga tccggtgctg aagaacgtgc cgatcagcga cgtcgcggcg
     2161 ctgcgcgact gggccctggc cgagaagatc gcgcttaccg tggtcggccc cgagcagccg
     2221 ctggccgccg gcgtggtgga cgacttccgt gcccacggcc tgcgcatctt cggccccacc
     2281 cgggcggcgg cgcagctcga aagctccaag gccttcagca aggccttcat gcagcgccac
     2341 ggcatcccga ccgccgcctt ccagaccttc accgacgccg ccgccgcgcg tgcctacgtc
     2401 gaacgcatgg gtgcgcccat cgtcgtcaag gccgacggcc tggccgctgg caagggcgtg
     2461 gtggtggcgc aaaccgtggc cgaggcgcac gccgccatcg acgacatgct gggcggcaac
     2521 agcctgggcg tggcgcacaa cgaaggcggc gcgcgggtgg tcatcgaaga attcctgccg
     2581 ggcgaggaag ccagcttcat cgtcctgtcc gacggcaaga gcgtgctggc actggccagc
     2641 agccaggacc acaagcgcct gcaggacggc gaccagggcc ccaacaccgg cggcatgggc
     2701 gcctattcac cggccccggt ggtcagcgcc gacgtgcacg cgcgcgtcat gcgcgaggtg
     2761 atcctgccca cggtgcgcgg catggagcag gacggcatcc ccttcaccgg ctttctgtac
     2821 gccggcctga tgatcgacgc ccagggccgg cccaggacgc tggaattcaa cacccgcatg
     2881 ggcgaccccg aaacccagcc catcctgatg cgcctgaagt ccgacctggt cgaggtgctg
     2941 ctggccgcca ccgccggccg gctgcacgag gtcgagctgc aatgggatcg gcgcgtggcg
     3001 ctgggcgtgg tgctggccgc cgcgggctac ccgctggcgc cgcgcaaggg cgacccgatc
     3061 accggcctgc ccgcgccgca ggaagacgtc agggtgtttc acgccggcac ggtggccgac
     3121 ggcgggcagg tgcgcaccgc cggtgggcgt gtgctgtgcg tcaccgcgct ggccgactcg
     3181 gtccggcagg cgcaggcgcg cgcctacgac gcggtgcgcg gcatccattt cgagggcatg
     3241 cagtaccggc acgacatcgg ccaccacgcg atccggcaga acccctgaca tgagcgcttt
     3301 ttccagccgg gtgcacgact acctgagcgg cctgcagtcg cgcatcaccc aggccatcga
     3361 ggccgccgac ggtcgggccg tgttcaggcg cgacgactgg cacaaggccg ctggcgaacc
     3421 gctgcagggc gcgggcagca cccgcattct ggagggcggc gcggtgttcg agcgcgccgg
     3481 catcggcctg tcgcaggtgc gcggcccgcg cctgccgccg tcggccaccc agcatcggcc
     3541 cgagctggaa ggcagcccgt tcgaggccat gggcgtgtcg ctggtgttcc accccctcaa
     3601 cccctacgta cccacggtgc acatgaacgt gcgcatgatc tcggccggcc acccgggcgc
     3661 cgaaccggtg tgctggttcg gcggcggcat ggacctgacg ccgtactacg gcttcgacga
     3721 agactgccag catttccacc gcacctgccg cgacgccctg gctcccttcg gcgacgacac
     3781 ctacccacgc ttcaaggcct ggtgcgacga ctacttcttc aaccgccacc gcggcgaggc
     3841 gcgcggcatc ggcggcatct tctacgacga cttcgtgggc ggcggcgccg agcagggtct
     3901 ggccctcacg cgcgcggtgg gcgaggcctt tttgcaggcc tacctgccga tcgtcgagcg
     3961 gcgcgccgcc accccctggg gcgagcgcga gcgccagcac cagctgcacc ggcgcgggcg
     4021 ctacgtcgaa ttcaacctgg tgtgggaccg cggcacgcac ttcggcctgc aatcgggcgg
     4081 gcgcaccgaa tccatcctgc tgtccatgcc gccgctggtg gcctggcagt accagcgcca
     4141 gcccgagccc ggttcgcccg aggcgcgcct gaccgagcat ttcctggtgc cgcgcgcgtg
     4201 ggtgtgacgc acgcgatgac gaatgacctc gcttcgattc gatgaccaat gacacatcag
     4261 agcctgtcgg atgccgggga ccgcgcgcag agcggcgcga gggcaaggtc attggtcatt
     4321 gaagccgcgc cagcggcgaa gtcattggtc atcgacccgt catgaccgcc ggcatccacc
     4381 gtgtcggcct gttcggcggc gctttcgacc cgccgcacca cgcccatgtg gcgctggcgc
     4441 aggcggcgat cgagcaactg gggctggagc gcctgcacgt ggtgcccacg ggggatgcct
     4501 ggcacaaggc gcgcgcgctc agcccggccg ccgaccggct ggccatgtgc cggctggcct
     4561 ttgccggcct gccccgggtg gcggtggacg aacgcgaact gcggcgcgcc ggcgccagct
     4621 acaccatcga taccctgacc gagctgcacg ccgaacaccc cgcggcacag tggttcctgc
     4681 tcatcggggc cgaccaggcg gcggcctttc acagctggcg acgggccgac gacatccttc
     4741 ggttagctac cattgtgata gctgttcgcg ctgatccctc gggcgtcggc gccgtttttg
     4801 atgcagaaca cccgctgccc ggcctggccg tcgcggcgga ccgcgtgcgg cggctgcaac
     4861 tgcccgccat gccgcacagc gccaccgccg tgcgccgcct ggcggcggcg gggcaaccga
     4921 tcgaacactt ggttcccgcg ccggtcgcgg ggtatattgc cgaccaccac ctctaccaaa
     4981 gccctgcctg atgagcgaaa ccgccgccaa gaaagacctg cagaaactcc agcgcgccat
     5041 cgtcgatggc ctggaagaca tcaaggccca ggacatccgc gtgttcaaca ccgaacacct
     5101 gtcgccgctg ttcgaacggg tgatcgtcgc caccggcagc tccaaccgcc agaccaaggc
     5161 gctggccacc agcgtgcgcg acaaggtgcg tgaagccggt tttgccaagc cgcgcatgga
     5221 aggcgaagac aacgccgaat ggatcatcgt cgactgcggc caggcggtgg cccatgtcat
     5281 gcagccggcc attcgccagt actacaagct ggaagagatc tggggcgaaa agccggtgcg
     5341 cctgaggaac gcggcgccac tgtccaggtc gtccgccgcc gccccggccg cgaagaaggc
     5401 ggccgccgcc gccccggccg ccaagaaggc agcgaccgcc gcccggaaga ccgcaaccgc
     5461 gggcacggcg gccaagaagg cgccggccgg gaaagccccc gccaggaagg ctgccgccac
     5521 gccggcggcg cctgccaagc gggcacctgc caagaaggcc gtggccggaa agaccgccgc
     5581 ggtcaagtct gccgccggca agcccgcccc gcgcagaaag ccggcctgat ccccgtttgc
     5641 cccctgcctg cggtcagcgg cctgcgacca actcgcgccc agcctgaccg atggccaacc
     5701 acgttgctgc gcttcgatga ccatcggttt gttcgtcttg ctggtgcagt gtttcgcgct
     5761 gtcaggtacc tacagcgagt cgccaagggg gaagcccgaa aaacgcttgc ggcggcgttg
     5821 cgcctcttgc caaggccgcc agcatgggct gcgagccgcg cctggccgca agcctctttc
     5881 tggcttccat aagtgcctga cagagcgaaa cactgcacca gcgccagcgg cgtcgcgggt
     5941 cgggtgtgtt gaacgcgatg cgtcgtgagc agccatgaag ctgatcgtca ttgccgtcgg
     6001 ccagcgcctg cccggctggg cgcagacggc ctgggacgac tacgccaagc gctttccgcc
     6061 cgaactgcgg ctgcagctca aggccgtcaa gaccgagccg cgcgccggtg gcaggaccgc
     6121 gccacaggcc atggcggccg agcgccagcg catcgaggcg gcgctgccgc gtggtgccca
     6181 tgtggtggtg ctggacgagc gcggcagcgc gctcaccacc gtggccctgg cggatcgcct
     6241 gcgccagtgg cagggccagg gcggcgacgt ggcgctgctg atcggcggcc ccgatgggct
     6301 ggacccggcc ctgcgccagg cggcgcatga acgcattcgc ctgtccgacc tgacgctgcc
     6361 gcacgccctg gtgcgggtgc tgctggtcga gcagctctac cgcgcctggt cggtcaacgc
     6421 gggccatccc tatcaccgag aatgaacgcc cggccgcgcc accgacggcc gtcgcttcgc
     6481 ctgttgcatg agcttcatct accttgcctc gcaaagtccg cgccggcgcc agctgctcga
     6541 tcagctcggc gtggcgcatc gcctgttgct gcccgatgac gcggccgccg ccgaggcgct
     6601 ggaggcggtg cgcccgggcg aggcgccggc cgcctacgtg cggcgcgtga cgcggctcaa
     6661 gctcgacgcc gcgctgcgcc gcctgcacca gcagggcctg ccgccggcgc cggtgctgtg
     6721 cgccgacacc acggtggcgc tgggccgcca catcctcggc aagccggccg acgcgcgcga
     6781 ggcggcggcc atgctgcgcc agctggccgg cggcacgcat cgcgtgatta cggcgctggc
     6841 gctgcaggcg ggcgagcggc gccacgaggc gctgagcgat tcacgggtgc gctttgcgcc
     6901 cgtgagcgaa accgagctgg ccgactacct ggccagcggc gaatggcagg gcaaggccgg
     6961 cgcctatgcc atccaggggc gggcggcggc tttcatcgcc cacatcagcg gcagctacag
     7021 cggcatcatg ggcctgcccc tgttcgaaac ccatcagatc atgcgtctgg cgcgcttgta
     7081 gaaagcgcca gcagctatac caaatgcagc aagacatcct catcaactgg tcaccccagg
     7141 aaacccgcgt ggcggtggtc gaaagcggcg cggtgcagga gctgcacgtc gagcgcgcgc
     7201 tcgagcgcgg cctggtcggc aacatctacc tgggcaaggt ggcgcgcgtg ctgccgggca
     7261 tgcagtcggc cttcatcgac atcgggctgg agcgcgccgc cttcctgcac gtggccgacc
     7321 tgtggcagaa gccggccaac ggcgacggcc tgccggcgca ccgcaccggg gcgcccacgc
     7381 gaccgatcga gaagcaggtg ttcgagggcc agacgctgat ggtgcaggtc atcaaggacc
     7441 cgatcggcag caagggcgcg cgcctgtcga cgcaggtcag tctggccggg cgcatgctgg
     7501 tgtttctgcc gcaggacgat catgtcggcg tgtcgcagaa gatcccggca gcccagcgcg
     7561 acgaactgcg cgggcgcgtg caggcgctgg tggccgcggg tggcgagggc ggccgcagcg
     7621 gcggcttcat tctgcgcacc aacgccgaag acgccagcga cgccgaactg gccgaggaca
     7681 tcgcctacct gcgcaagacc tgggcgcgca tccgcgagtc ggccacgcgc cagccgccgg
     7741 ccacgctgct gcacgaggat ctgagcctgc tgcagcgcgt gctgcgcgat ctgacgagcg
     7801 aggccacgca gtcgatccgc atcgattcgc gcgagcagtt cgatggcctg ctggccttcg
     7861 gccaggagtt catgccggcc gccgcggcgc gcctgtcgca ctacaagggc gagcggccga
     7921 tcttcgacct gttcaacgtc gacgaggaaa tcaccaaggc gctgggtcgg cgcgtcgacc
     7981 tgaaatcggg cggctacctg atcgtcgatc agaccgaggc gctgaccacc atcgacgtca
     8041 acaccggcgg cttcgtcggc gcgcgcaact tcgacgacac ggtgttcagg accaacctgg
     8101 aggcggcgct ggccatcgcc cgccagctgc gactgcgcaa cctgggtggc atcatcatca
     8161 tcgacttcat cgacatgacg cagcccgagc accagcaggc ggtgctggcc gagctgcgca
     8221 agcagctggg gcgcgaccgc gtcaagaccc aggtgggcgg cttttcgccg ctcggtctgg
     8281 tggagatgac gcgcaagcgc acgcgcgaat cgctggcgca gctgctgagc gagccgtgca
     8341 cgcagtgcca gggcagcggc cgcgtgcgca ccgcccgcac cgtcgcctac gacatcctgc
     8401 gcgaaatcct gcgcgaggcg cgccagttca accccaagga gttccgcatc gtcgccgctc
     8461 cggccgtggt cgagatgctg ctggacgagg aaagccagca tctggcaggc ctgtccgact
     8521 tcatcggcaa gccgatttcg ctgcaggccg aatccagcat cgggcccgac cagtacgaca
     8581 tcgtgttgct gtaggcggcg ccgcacgcgc ctggtgcgcg ccgggcccgc accggccggg
     8641 tcgtggcatc agccggtgtt gcgcagcccg gcggcaatgc cgttgatgca caggtgaatg
     8701 ccggtctgca cgcgctcgtc gccgggttgg gcgcgccagc ggcgcagcag ctcgacctgc
     8761 aggtgatgca gcgggtcgat gtagggaaag cggtggcgga tcgagcgcgc cagcgcggcg
     8821 ttgccggcca ggcgctggcg gctgccggtg atctgcgtca gcgcgtcgtt ggtgcgctgc
     8881 cattcggcct cgatggcgcc gaacacgcgg cgccgcagcc ggcggtcggc caccagttcg
     8941 ctgtagcgcg aacccagcgc caggtcgctc ttggccagca ccatgtccag gttcgacagc
     9001 agggtgcgaa agaacggcca ctcgcggtgc atgcgccgca gcagcgccag acgcgcgcgc
     9061 ggcggggcgg cgggatcgtc catgaaggtc tgcacggcgg cgccgaagcc gtaccagccg
     9121 ggcagggtca ggcgacactg gccccagctg aagccccagg ggatggcgcg caggtcctcg
     9181 atgcgatcgc tcgccttgcg gctggcgttg gtgcgctgcc attcggcctc gatggcgccg
     9241 aacacgcggc gccgcagccg gcggtcggcc accagttcgc tgtagcgcga acccagcgcc
     9301 aggtcgctct tggccagcac catgtccagg ttcgacagca gggtgcgaaa gaacggccac
     9361 tcgcggtgca tgcgccgcag cagcgccaga cgcgcgcgcg gcggggcggc gggatcgtcc
     9421 atgaaggtct gcacggcggc gccgaagccg taccagccgg gcagggtcag gcgacactgg
     9481 ccccagctga agccccaggg gatggcgcgc aggtcctcga tgcgatcgct cgccttgcgg
     9541 ctggccggcc gcgagccgat gttgagctgg gcgatctcgc ggattggcgt ggcggtgaag
     9601 aagtagtcgg caaagccggg cgtgtcgtac accagggcgc ggtaggccgc catgctggcg
     9661 cgcgagagct gctcggccgc ctgcaggaat tcgtcggggg cggcctgggt gggttgcagc
     9721 agcgtggctt ccagcgtggc ggcgaccagg gtttccaggt tgcggcggcc gatttcgggg
     9781 ttggcgtact tggaggcgat gacctcgccc tgctcggtca gtcgaatctg cccgcgcacc
     9841 gtgccggggg gctgcgccat gatggcctgg tagctgggtc cgccgccgcg gcccacggtg
     9901 ccgccgcggc cgtggaacat gcgcaggcgg atcggcgctt cggcgtgctc gcgattgagt
     9961 tcgtcgaaca gcgccaccag ggcgatctcg gcccggtaca gctcccagtt gctggtgaag
    10021 atgccgccgt ccttgttgct gtcgctgtag cccagcatga tgtcctgctc ggcaccactg
    10081 gcacgcacca gcgcggcgat gccggggatg gcatagaagg cgcgcatgat gggggcggca
    10141 tggcgcaggt cggcgatggt ttcgaacagc ggcaccacga tcaggtcggc cgtggcctcg
    10201 ccccccaggg tgccgcgcat caggccgact tccttctgca gcaacagcac ctccagcagg
    10261 tcgctgacgg tttcggtgtg gctgatgatg tagtggcgga tggcgtcgtg gccgtggctg
    10321 tggcgcagcg ccagcgcggc ctcgaagatg gccagctcgc cctgggtgtg cgcgctgtac
    10381 tgcccgccca ccacccgcaa tggccgtgca tcgttcagca ggcgcagcag cagggcgcag
    10441 cgcgcgtgtt cgtccaggct ggcgtagtcg ggcgccagct gcgcgcgcgc cagcagctcg
    10501 gcgaccacgc gctcgtgctg gtcggagctc tggcgcaaat ccaccgtggc caggtgaaag
    10561 ccgaacacct cgaccgcacg catcagcggc tgcaaccggt cggccaccag ggcctggcca
    10621 tcgtgggcgc gcagcgaacc ctcgatggtg cgcagctcgg ccagcagttc gtcggcccgc
    10681 gcataagcgg ccagttgcac cgcgggcgga cgcggcgcgc cgcgcccggc cagccgctcc
    10741 agcgtggccg acaggcgggc gtagatgccg gtcagtgcgc gccggtaggg ctcgtcgctg
    10801 cggtgcgtgc tggtgtccgg cgagcgctgc gccagcgccg ccagggcggg cgataccgcg
    10861 gtcagccgcg acgacagcga cagcgtgcga cccagctcgt gcacttcgtc caggtagtgc
    10921 agcagcgccg cttcggcctg gcggcgcagc gccaggtgca gcgtctgtgc gctgacgttg
    10981 gggttgccgt cgcggtcacc accgatccac tgccccatgc gcagaaagct ggccaccggc
    11041 cgtgcggcgc cgagcgcctg ctccaggtcg gcgtacaggc gcggaatctc gcgcaggaag
    11101 gtggactcgt aataggagag ggcgttttcg atttcgtcgg ccacgctcag gcggtcgtag
    11161 cgcagcaggc gggtttgcca cagcagcacc acgcgggcgt gcaggcgggt ttcgttggcg
    11221 gcaagctggc gtggcgacag cacgtcgcga ccgccgggct ggcacagggc gcgttcgcgc
    11281 aactcgtcgc gctgggccag caactgggcg atctcgcgct cggcgtcgag gatgctcttg
    11341 cgctgcacct cggtcgggtg ggccgtgagc accggggcga cgtagctgtc cgacagcgtg
    11401 cgggcgatcg attcggcgtc gatgccggcg gcgtgcagcc tttgcagggc gacctcgatg
    11461 ccaccttccg gcgcactgcc ggcgcgttca tgcacggcgc ggcgacggat gtggtggcgg
    11521 tcctcggcca ggttggccag caggctgaaa caggtgaagg cacggatcac gctcaccgtc
    11581 tgctcaccgc tcaggccatt gagcaggcgg cgcagggtgt tgccggcgcg ctggtccgcg
    11641 tcgcgccgga acgccaccga cagggtgcgg atgcgttcca ccagttcgta ggtggcggtg
    11701 ccttcgtgct cgcggatcac atcgcccagc aggcgcccca gcaggcggat gtcctggatc
    11761 agcggcagct ccttgtccga gcggctgggg cgtcgggcgg ggctggcggt ggggatggcg
    11821 gcaggggcgg ttcgggtagt gcgcatgggc gggattgtcc acgcggcgat ggcgccgcgg
    11881 gccgccgcgc gcgggtcagg gcgcgatctt ctttttcagc gcctgcgcca ccggctgtgg
    11941 caggctgggc agggacttga gcagccaggg cagtaccttg cgtttggcac cgcccagcga
    12001 ctcgggcacc atggcctcga tcaggtgcgg cccgctctga cccagggcgt attccagcgc
    12061 ctggcaaaaa tcctcggccg tgtgggtgcg cgccgcgtgc acgcccatgc cttgggccag
    12121 ttgcgcaaag ctgagcaccg ggccgtgcag gtcgagctgc gacttggcct tggggccggc
    12181 ctcgtcggcg cccacgcggt cgagctccac gttgagcacc gagtagctgg cgttgttgaa
    12241 gatgatggcc gtgacgttga ggttttcccg cgccatggtc cacagcgcct ggatggtgta
    12301 catggccgtg ccgtcgccaa tcagcgcgat caccgggcgc tgcgggcagg caatcgccgc
    12361 gcccacggca ttgggcaggc cctggcctat ggcgccgccg gtcagcgtga tgaggtcgtg
    12421 gcgcgggcag ccagccgtca tcacgcccag catcaggccg ctggtgatgg cctcgtcgat
    12481 gacgatggca cgctcgggca gcaatgcgcc cacggccttg cagaccttgg gcgcggtgag
    12541 cctgcccgtg gggcgattgg ggcgctgcgg cgcctgcagg cgtggctggg cagcgcccgc
    12601 gcccagcgcg gcggcgagtt gctccaggct ggcggcggcg ttctgctcgg gcgtggccag
    12661 ggtgtgcacc tggcaggtct cgggcaccag gtagctcctt ttgccggggt aggcgaagaa
    12721 ggatacgggc gccttggcgt cgaccaggat gagctgctcg atgggagcga gctgcacgcc
    12781 cgccatctcg gccaggtagg cgatgcgctc gacgaaaggt aggcccgcgc cgcgctccat
    12841 gcgcgtgggg aagcattcgg ccagcagctg cacgccgctg tgcgcggcaa tgcgcgcggc
    12901 ggccagcagg gcgggctcgc gcagcgcctg gccgccgagc agcagggcgg ttttcttgcc
    12961 gctgcgaatg gcgtgggcga tcgtctgcac cgtggcgtcg tccgccgcct ggggcttgag
    13021 ggctggcggc ggcgcgcagg gctggccgcc ctcgccccag gacacgtccg ccggtaggat
    13081 gagcgtggcc acctggcccg gcaggccgcg tgctgccact atggcgtcca cggcatcctc
    13141 gcccagctgc gccgtgctct tggacgtgcg cacaaagccc ggcgagacgt tgcgcgccac
    13201 ggtttcgatg tcgctctgca gctgcgcgtc gtactgggtg tggtaggtgg cgtggtcgcc
    13261 cacgatgttg acgaccggca ccttgccctt gcgcgcgttg tgcaggttgg ccaggccgtt
    13321 gcccaggccg cagcccaagt gcaagagcac ggcggcgggc ttgccggcca tgcgtgcgta
    13381 gccgtcggcc gcgcccgtgg ccacgccctc gaacagcgcg agcacggcgc gcatgcgcgg
    13441 ctcggagtcg agcgcggcga cgaagtgcat ctcgctggtg ccggggttgg aaaaacatac
    13501 ttcgacgccg gcgtcggcca gggtcttgat cagggcttgt gcgccgttca tgggtactcc
    13561 ttgattcata gctgctggcg cttgatgggt gggcgctgga gccggttctt attcaaactt
    13621 tcagcggttg cgccagaaca tgacgtccag catcctgcgc gccatgcccc ggtagggtgg
    13681 catcaaaaag tccacggtgc gcagcgggcc ctggcggaac accgggcgca gcttggagaa
    13741 ggtcaggaac ccttcatggc cgtggtaatg gcccatgccg ctgttgccca cgccgccaaa
    13801 gggcagactg tgctggccca cgtggagcag cgtgtcgttg agcgtgacgc cgcccgagag
    13861 cgtctgctgc aagtagcgct gctgcagcgc cttgtcgtcg ctgaacaggt agagcgcgag
    13921 cgggtgcggc cggtcgttga tgtagcgcag cacctcgtcg tgggtttgat aactgaggat
    13981 gggcagcagc gggccaaaga tctcgcgctg catgacctgc atgccgtcgt gcacgcccag
    14041 cagcgccacc ggaggcatga tgcgccgcgc cgcatcgccc tgttggccgg gcgccagcgg
    14101 cacgacctgg gcgccctggg ctaccgcgtc ggccagcatg gcctgcaggc ggtcgtactg
    14161 gcgctggtcg atgatggcgg tgtagtcgcc gctcgtgagg tcggggtagc gctgggcgac
    14221 gatgcgctgc gccgcttcga tgaaggcctg ctcctggccg gcgggcacga acatgtagtc
    14281 cacgttggtg cagatttgcc cggcgttgag catcttgacc cacagaatgc gctcggccgc
    14341 cttgtgcatg gcgtagccgg gcgcgacgat ggcgggcgac ttgccgccca gctccagcgt
    14401 caccggggtc aggttggccg cgcagttggc catcacggcc ttgccggtct gcggcgagcc
    14461 ggtgaagaac aggtggttga acggcagctg cgtgaacagc ggcccgagcg actggcccgg
    14521 ccccgcgtcg ctgtcggcaa acaggctcac cttgtcggcg gcaaagtatt ttggcagtag
    14581 ctcttgcagc aggcgcgcca ggcgttcgga gttctccgac atcttgatca tggcgcggtt
    14641 gcccgcggcc agcgcgccta tgaggggctg caccaccatg gcgatgggaa agttccacgg
    14701 cgcgacgatg ccgaccacgc ccagcggctg gggctggaca aaggcgctgg ccagcgggta
    14761 ctgggtcatg tccaggtggc ggctttgctt tttcatccag cgcggcagcc ggcgtatggc
    14821 gtccagcgcc ccttcctggc cctgcagcag ctcggcaaac tgggtttcaa aatggctgcg
    14881 gcagccgaag tcgcggttca cggcatcgac cagcgcgtcg cggttctcga ccagcaggcg
    14941 gtgcagggcg cgcaggtcgg cgcggcgctc ttgcaagctg gggtaacggt tctgggcaaa
    15001 ggccgcctgc tgcgcggcaa agcagtcgcg cagattggcg g
//
LOCUS       DAOQTZ010000068        14940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4528_length_14940_cov_14.311320, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000068 DAOQTZ010000000
VERSION     DAOQTZ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14940
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4528_length_14940_cov_14.311320"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1483)
                     /gene="paaF"
                     /locus_tag="PKN51_09040"
     CDS             complement(176..1483)
                     /gene="paaF"
                     /locus_tag="PKN51_09040"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNT85285.1"
                     /translation="MNTTTKTFPLEPIEKASIDELRALQLQRLRATLAHAYAHSPVYK
                     AKFDAAGVHPDDCRSLEDLARFPFTTKQDLRDHYPFGMFAVPREQLVRIHASSGTTGK
                     PTVVGYTRKDIDTWADVVARSFRAAGARPGDMLHQSYGYGLFTGGMGAHYGAERLGLT
                     VVPFGGGQTEKQIQLILDFKPDIITVTPSYMLAICEQVEHMGLDPKELSLRIGIFGAE
                     PWTNEMRQAIEKRMAIDAVDIYGLSEVMGPGVANECVETKDGPTIWEDHFYPEIIDPE
                     TGAVLPDGQEGELVFTSLTKEALPIIRYRTRDLTRLLPGTARTMRRMQKITGRTDDMM
                     IIRGVNVFPTQIEELILKRPELSPNYQCVLTREGPLDELKVQVEVAPGLDPQHIGARA
                     AAKLLQHEIKIYVGSSVEIELKPEGGIERSVGKARRVVDLRKK"
     gene            complement(1480..1947)
                     /gene="paaI"
                     /locus_tag="PKN51_09045"
     CDS             complement(1480..1947)
                     /gene="paaI"
                     /locus_tag="PKN51_09045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HNT85286.1"
                     /translation="MRHNPAAQALAQRVGQAMFAADRASRETMGMELLACEPGRALMR
                     MTVQPLHLNGHQICHGGFIFTLADSTFAFACNSRNHNTVAAGCSIEFLKPAHAGDVLT
                     CEGVEQVQSGRHGVYDMRVTNQHGDVVALFRGKSAQIKGHVLADEPTSQESGA"
     gene            complement(1950..2753)
                     /gene="paaG"
                     /locus_tag="PKN51_09050"
     CDS             complement(1950..2753)
                     /gene="paaG"
                     /locus_tag="PKN51_09050"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HNT85287.1"
                     /translation="MTQPTEPDVLYAEEGAVAILTLNRPASLNSFTRQMHRDLWAALD
                     RLEANPAIRALVLTGAGRGFCAGADLSEFDFADGPNLQQRADPGPVIERAFNPTTRRL
                     INVRVPTVCAVNGVAAGAGASVAMACDITIAAPGASFIQAFSKIGLVPDSGGTWLLPQ
                     RVGLARAMALCLTGDKLSAQDAKAMGMIWDVADDALAAAMALAGRLAAMPTRALVATR
                     HLLRGAHTHSLDEQLDLERDVQSRLGFTHDYIEGVMAFLQKRPAQFRGE"
     gene            2880..3659
                     /locus_tag="PKN51_09055"
     CDS             2880..3659
                     /locus_tag="PKN51_09055"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNT85288.1"
                     /translation="MTYELITTRVEGGQVGVITLNRPKQLNALNSQLMVELGQALKAF
                     DADPAIGCMILTGSEKSFAAGADIGAMASYSFADVYRDDYITRDWETIRSIRKPVIAA
                     VSGFALGGGCELAMMCDFIIAADNAKFGQPEIKLGVIPGAGGTQRLPRAVGKSKAMDM
                     VLTARMMDAQEAERAGLVSRVVPLDKLMDECLAAALTICSFGQLAVMAAKESVNRAFE
                     GTLADGVMFERRLFHGLFATQDQKEGMDAFVNKRPAAFKHQ"
     gene            3695..3771
                     /locus_tag="PKN51_09060"
     tRNA            3695..3771
                     /locus_tag="PKN51_09060"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3729..3731,aa:Met,seq:cat)
     gene            4287..4517
                     /locus_tag="PKN51_09065"
     CDS             4287..4517
                     /locus_tag="PKN51_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85289.1"
                     /translation="MTAAVREKFSSQAAPDVLAALRQIAETQGRQFQAVLDDALRDYI
                     DRQQKERPRRHVMASFASSLDEFDSLYRELAK"
     gene            4521..4901
                     /locus_tag="PKN51_09070"
     CDS             4521..4901
                     /locus_tag="PKN51_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNT85290.1"
                     /translation="MPRDHLTVADVLGMHTVLMQRYGGAPGVRDPGALEAALFRPQTG
                     YYDDIVAEAAALLESLAINHPFVDGNKRIAFAAADVFLRINGWRLHRAPMQIHAEVIE
                     MFETRTFDIAHLDPWLRSFATLAE"
     gene            complement(5197..5289)
                     /locus_tag="PKN51_09075"
     tRNA            complement(5197..5289)
                     /locus_tag="PKN51_09075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5253..5255),aa:Ser,seq:gct)
     gene            complement(5317..6588)
                     /locus_tag="PKN51_09080"
     CDS             complement(5317..6588)
                     /locus_tag="PKN51_09080"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNT85291.1"
                     /translation="MALIVQKYGGTSMGSTERIAQVAKRVAKWARAGHRMVVVPSAMS
                     GETNRLLALAKELAPGKPTQDYYREQDMLAATGEQASSALLAIALQAEGQPAVSYAGW
                     QVPIRTSNAYTKARIDSIDDQRVRADLDAGKVVIITGFQGVDADGHITTLGRGGSDTS
                     AVAVAAALKADEAMIFTDVDGVYTTDPRIVPEARRLASLSFEEMLEMASLGSKVLQIR
                     SVEFAGKYKVPLRVLSSFTPWDIDLAEEARSGTLITFEEDEHMEKAVVSGIAFNRDEA
                     KISVLGVPDKPGIAYQILGAVADANIEVDVIIQNISKDGKTDLSFTVNHGDFARAMDL
                     LKDKVAPALGAADVVGDPRICKVSIVGIGMRSHVGVASKMFRALSEEGINIQMISTSE
                     IKTSVVIDEKYMELAVRALHRAFDLDQPSAA"
     gene            complement(6649..7593)
                     /gene="tilS"
                     /locus_tag="PKN51_09085"
     CDS             complement(6649..7593)
                     /gene="tilS"
                     /locus_tag="PKN51_09085"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNT85292.1"
                     /translation="MDAFAAEAPALPLAVALSGGADSTALLLACAARWPGQVQAWHVH
                     HGLQPAADDFERHCSALCARLDVPLLRRRVDARPARGQSPEDAARRHRYEAFDALALA
                     SQAQAAIKTVVIAQHADDQVETILLALSRGAGLPGLSAMPARWQRGDVAYRRPLLGVP
                     AAAIRAWLRARGQGWVEDPSNADERYTRNRIRARLLPALQACWPAYRTTFARSAAHAA
                     EAQQILADVAAQDLAHCGVPPRLDALQTLSAPRQANLLRHWLASTHAQTPSSAQLAAL
                     LAQVAAARTRGHRIQLKVGAGRVSRRGAVLHWAPDAER"
     gene            complement(7780..7856)
                     /locus_tag="PKN51_09090"
     tRNA            complement(7780..7856)
                     /locus_tag="PKN51_09090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7820..7822),aa:Val,seq:gac)
     gene            complement(7898..10114)
                     /locus_tag="PKN51_09095"
     CDS             complement(7898..10114)
                     /locus_tag="PKN51_09095"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNT85293.1"
                     /translation="MKTLSVAPAGTRQSVADVVVATGGGQPEAGELRARARAFAEPLL
                     AGETTASGENVLTHADAVADILAGIGGSEAIQAAAYLSYASAQLNRPEEVIGKAFGDS
                     LARLALETARLEQLQQRSRASRQQLSADTEQASQQTEFVRKMLLAFSRDLRVVLLRLA
                     SRLQTLRYYAAARRPPPPGLLRESHEVFAPLANRLGIWQIKWEMEDLVFRAQEPDTYR
                     HIASLLDEKRVERELALEQRRAAIEAALRAQGIDASVSGRPKHISSIVRKMRGKSLGF
                     EQVFDVRALRILVPTVVDCYTALGWVHQHFTPVPEEFDDYIARPKPNGYRSLHTVVRD
                     PDGRAWEIQIRTHDMHQHAEHGVAAHWAYKEAGTKGYAGVSAAGEYDAKIAVLRQLLA
                     WERELSGAAPQHGLFDDRIYVLTPEAAIVELPAGATPVDFAYTLHTELGHRCRGARVD
                     GAMLPLHTPLKNGQTVEIVAAKEGGPSRDWLNPELRYLASSRARAKVRAWFNAQHTRE
                     TVARGREMVEKLLQREGRTALSLEHLAAELGFKTPEALFETVGKDELSLRAIEAVLRP
                     PEPTPAPDDQVLLRKARAGSAGGRGGVLVVGLDSLLTQPARCCKPAPPDDIAGYVTRG
                     RGVTIHRRDCASFRDLQAREPERVIEVGWSTPATGHLPAYPVDVAVEALDRQGLLRDI
                     SDVFAREKMNVIGVQTQSVKGWAWMTFTVEVSDTQRLAFVLRQVQDVPGVRVAKRR"
     gene            complement(10111..11037)
                     /locus_tag="PKN51_09100"
     CDS             complement(10111..11037)
                     /locus_tag="PKN51_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT85294.1"
                     /translation="MTEPVLHHVSCPDASGGHRMACWQWGDAAAAHLVVCVHGLSRQG
                     RDFDVLARALLARAAARGQPLRVVCPDVVGRGESDWLKEPMGYQFPTYVGDMLTLLGT
                     LAQQAPIGRFDWVGTSMGGLIGLMLAGAPDLPLPAPLRRLVLNDVGPVIEWQALQRIG
                     QYLGKSGPFESVEAGAACLRALAPGFGPHTDAQWLALSRPMLRARPEGDWRLHYDPAI
                     AVPYAAVTPESSAQGEALLWQLYDRVKADTLLLRGAESDLLSAATASAMQARGPRPRL
                     LEFAGVGHAPTLVADDQVAAVLDFLLPAGEAP"
     gene            complement(11223..11966)
                     /locus_tag="PKN51_09105"
     CDS             complement(11223..11966)
                     /locus_tag="PKN51_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541752.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNT85295.1"
                     /translation="MNHRLVRAARALALLLAIAAPAAHAQTHSPATPMSPTPNVPQNV
                     IQLSASGQVEVVQDLLTLTLGTAREGADAAGVQAALRQALDEALRQVGASAPADQMEV
                     RTGAFRVHPRHDRNGRISGWQGRAELVLEGRDFARITQAAARATTMVIDQLAFGLSRT
                     QRARAETEAQTQAIERFKARASDVARAFGFAGYTLREVSVSGDAGDIAPRGFGMASRM
                     EAMAADAPVPVEPGRSLVQVTVSGTVQAH"
     gene            complement(12022..12336)
                     /locus_tag="PKN51_09110"
     CDS             complement(12022..12336)
                     /locus_tag="PKN51_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85296.1"
                     /translation="MNRWICLLGIWGALALPMAVRAQAVGEASESGPSGAPPAVSEAA
                     LARSPLWQAVESMFRQREVGPVPDRRLDAQQRQQLREQVRRAAARGQRHGVAEVTQLG
                     SR"
     gene            12506..13237
                     /gene="ompR"
                     /locus_tag="PKN51_09115"
     CDS             12506..13237
                     /gene="ompR"
                     /locus_tag="PKN51_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HNT85297.1"
                     /translation="MNQSANRTDKIVIVDDDARIRDLLRRYLAQEGFEVIVAEDAKAL
                     ARVMLRDTIDLIVLDLMMPGEDGLSVCRRLRAAGDKTPIIMLTAKGEDVDRIVGLEVG
                     ADDYLGKPFNPRELLARIHAVLRRRPPLEAPGAPSAENESVAFGPFQFDLGARTLHKN
                     GEEVPLTTGEFAMLKALVRHPRQPLSREKLAQLARGREFEPFDRSLDVQVSRLRKLIE
                     QDAASPRYIQTVWGVGYVFVPDGTG"
     gene            13351..14739
                     /locus_tag="PKN51_09120"
     CDS             13351..14739
                     /locus_tag="PKN51_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT85298.1"
                     /translation="MEPEVEARAPLAFSLFWRTFFLLALLLLGTTVGWYQMFRALEYE
                     PRVIGNARQIASLVNLSRAALIHSDAIARVSLIKTLAEQEKVRIAPREPGDRFELFNQ
                     TVLERRMSGEIVARLGLGTVVASRVNGEEGLWVGFSIEGDSYWLLMDRSRIGTLLSGS
                     AWLLWLATLGAASLLGAALLAGYINRPLRQLSNAAARVREGDYLEGRLDETTRAREIR
                     AVNIGFNRMAEELSRIEQERAEMLAGISHDLRTPLARLRLETEMSVPDAEAREHMVAD
                     IAQVDTIIDKFLDYARPDHVSLQALPLAALAQAAAQPFASREDMQVQIDVPPELRVMG
                     DEVELSRVLANLLENARRYGKSADSGIARVRIAATARDQWVTLRVRDHGPGVPEEQLA
                     VLTRPFFRGDTARTEATGAGLGLSIVAKMVRHMGGELNLTNSPSGGLMAVIRLPQASD
                     RRADHGSLRKRR"
BASE COUNT         2288 a         5107 c         5197 g         2348 t
ORIGIN      
        1 tgcccgtacg catccgcatc acccaggggc cggacacctt catggacctg ctggcagaca
       61 ccatcgaaca gcgctgatgc tattgttatg atagcttttg gcgcttgctg cgtgcgtgcc
      121 agaaggcaaa acatccagaa gcgaggcggt gggcgtcagg cccgtccagg gcccctcact
      181 tcttgcgcag atcgaccacc cgccgcgcct tgcccacgct gcgctcgatg ccgccttcgg
      241 gcttgagttc gatctcgaca ctgctgccga cgtagatctt gatctcgtgc tgcagcagct
      301 tggcggcggc gcgggcgccg atgtgctgcg ggtccagacc gggcgccacc tcgacctgca
      361 ccttcagctc gtccagcggc ccctcgcgcg tcagcacgca ctggtagttg ggcgacagct
      421 cggggcgctt caagatcagc tcctcgatct gcgtcgggaa cacgttgacg ccgcggatga
      481 tcatcatgtc gtcggtgcgg ccggtgatct tctgcatgcg gcgcatggtg cgcgccgtgc
      541 cgggcagcag gcgcgtgagg tcgcgcgtgc ggtagcggat gatgggcagc gcctccttgg
      601 tcaggctggt gaacaccaac tcgccctcct ggccgtcggg cagcacggcg ccggtttcgg
      661 ggtcgatgat ctcggggtag aaatggtctt cccagatggt ggggccgtcc ttggtttcga
      721 cgcactcgtt ggccacgccg gggcccatca cctcggacag gccgtagatg tcgaccgcgt
      781 cgatcgccat gcgcttttcg atggcctggc gcatttcgtt ggtccagggt tcggcgccga
      841 agatgccgat gcgcaagctg agctccttcg ggtccagccc catgtgctcg acctgttcgc
      901 agatcgccag catgtagctg ggcgtgacgg tgatgatgtc gggcttgaag tccaggatca
      961 gctgaatctg cttttcggtc tggccgccgc cgaagggcac caccgtcagg cccaggcgct
     1021 cggcaccgta gtgcgcgccc atgccgccgg tgaacaggcc gtagccgtag ctctggtgca
     1081 gcatgtcgcc ggggcgcgcg ccggcggcgc gaaacgagcg cgccaccacg tcggcccagg
     1141 tgtcgatgtc cttgcgggtg tagccgacca cggtgggttt gccggtggtg ccgctggagg
     1201 catggatgcg cacgagctgc tcgcgcggta cggcgaacat gccgaacggg tagtggtcgc
     1261 gcaagtcctg tttggtggtg aacgggaacc gggccagatc ctccagcgac cggcagtcgt
     1321 cggggtgcac gccggcggcg tcgaacttgg ccttgtagac cggcgaatgc gcgtaggcgt
     1381 gcgccagcgt ggcccgcagc cgctgcagtt gcagcgcacg caactcgtcg atgctggcct
     1441 tttcgatcgg ctccagcggg aaggtcttgg tggtggtgtt catgcaccgc tctcctggct
     1501 cgtgggttcg tcggccagca catggccctt gatctgcgcg ctcttgccgc gaaacagcgc
     1561 caccacatcg ccgtgctggt tggtcacgcg catgtcgtag acgccgtggc ggccgctttg
     1621 cacctgctcg acgccctcgc aggtgagcac gtcgccggcg tgcgcgggct tgagaaactc
     1681 gatgctgcaa ccggccgcca cggtgttgtg gttgcggctg ttgcaggcaa aggcaaaggt
     1741 cgaatcggcc aacgtgaata tgaaaccgcc gtggcagatc tggtggccgt tcagatgcag
     1801 cggctgcacc gtcatgcgca tcagggcacg acccggctcg caggccagca gctccatgcc
     1861 catggtttcg cgcgaggcgc ggtcggcagc gaacatggcc tggccgacgc gttgcgccag
     1921 cgcctgggcc gcagggttgt ggcgcatgtt cattcgcccc tgaactgcgc cgggcgcttt
     1981 tgcagaaagg ccatcacgcc ctcgatgtag tcgtgcgtga agcccaggcg cgactgcacg
     2041 tcgcgctcca gatcgagctg ctcgtccagc gaatgggtgt gggcgccgcg cagcaggtgg
     2101 cgcgtggcca ccagcgcgcg cgtgggcatg gcggccaggc gtccggccag cgccatggcg
     2161 gcggccagcg catcgtcggc cacgtcccag atcatgccca tggccttggc gtcctgggcg
     2221 ctcagcttgt cgcccgtcag gcacagcgcc atggcgcgcg ccagaccgac gcgctgcggc
     2281 agcaaccagg tgccgcccga atcgggcacc aggccgatct tgctgaaggc ctggatgaag
     2341 ctggcgccgg gcgcggcgat ggtgatgtcg caggccatgg ccaccgaggc gcccgcgccc
     2401 gctgccacgc cgttgaccgc gcacacggtg ggcacgcgca cgttgatcag gcgccgcgtg
     2461 gtggggttga aggcgcgctc gatcaccggg ccggggtcgg cgcgctgctg caggttgggg
     2521 ccatcggcaa agtcgaactc cgacaggtcg gcaccggcgc agaagccacg cccggcgccg
     2581 gtcagcacca gggcgcggat cgcggggttg gcctccaggc ggtcgagcgc ggcccacagg
     2641 tcgcggtgca tctggcgcgt gaagctgttg agtgaggcgg ggcggttgag cgtgaggatg
     2701 gctacggcgc cttcctccgc atagagcacg tcgggctcgg tcggctgggt catgggttgt
     2761 ctccttgggt cgcgaattta ccattgaccg actgtgcggt cggctaacct gcacggcagc
     2821 cgtcgccgct ggttatagtg cctcttgcat cgcttcacac gacctgttca ggagaaccca
     2881 tgacttacga actcatcacc acccgcgtcg aaggcggcca ggtgggcgtg atcacgctca
     2941 accggcccaa gcagctgaac gcgctcaaca gccagctgat ggtcgaactg ggccaggcgc
     3001 tcaaggcttt cgacgccgac ccggccatcg gctgcatgat cctgaccggc agcgaaaagt
     3061 ccttcgccgc cggcgccgac atcggcgcca tggccagcta cagctttgcc gacgtctacc
     3121 gcgacgacta catcacgcgc gactgggaaa ccatccgcag catccgcaag ccggtgatcg
     3181 ccgccgtcag cggttttgcc ctgggcggag gctgcgagct ggccatgatg tgcgacttca
     3241 tcatcgccgc cgacaacgcg aagttcggcc agcccgagat caagctgggc gtgatccccg
     3301 gtgccggcgg cacccagcgg ctgccgcgcg cggtgggcaa atccaaggcc atggacatgg
     3361 tgctgacggc gcgcatgatg gacgcccagg aagccgagcg cgccggcctg gtcagccgcg
     3421 tggtgccgct ggacaagctg atggacgaat gcctggccgc cgcactgacc atctgcagct
     3481 tcggccagct ggcggtgatg gcggccaagg aatcggtcaa ccgcgctttc gaaggcacgc
     3541 tggccgacgg cgtgatgttc gagcgccggc tgttccacgg gttgttcgcc acgcaggatc
     3601 agaaagaagg catggacgcc ttcgtcaaca agcgccccgc tgcgttcaag caccaataga
     3661 gcgcagcgcc aatgccctat aatgcgcgtt tgccggtggt atagctcaga cggttagagc
     3721 gtgggattca taaccccaag gtcggtggtt cgattccacc taccaccacc acacacaagg
     3781 gctcgggttt tctccgggcc cttttttctt gcctgagagc accacgccac gcggggttgc
     3841 ggtcacctgt gctgcgggtg gccaccagtc gaaacgccgg cagtcggccg ctttctcgcc
     3901 agggccgtct ctattccttg tctggcctgc ggtaggcgca gtcgttgata tacaaaatca
     3961 acaacctacg cgcgcctgtt cagcgtcaac caaggcgacc agtcagccca ggaggctgtc
     4021 gggcttggaa cgccgaaagc gttctcgaag agcggcgtag ccaaaatcgg ccccagcgag
     4081 accagttttt gccggatttg cagcccaata gccgagctat tgggcaaaaa gccggtgaaa
     4141 aatgggccgc tgcggtcgat ttgcagccgg cgatttccaa gtccgcagcc tcctagggtc
     4201 aggcatcgaa ccaggtcgga cagagtggcg cagggcctca tcttgatata gcatgaaacg
     4261 tcaagatgaa tcatgatgga gaggccatga ctgctgccgt ccgcgagaaa ttttcttccc
     4321 aggccgcgcc cgacgtgctc gcggcgttgc gccagatcgc cgagacccag ggacgccagt
     4381 tccaggcggt gctggatgac gccctgcgcg actacatcga ccggcaacag aaggaacgcc
     4441 cccgtcgcca cgtcatggcc tcgttcgcgt ccagcctgga tgagttcgac agcctctacc
     4501 gtgaactggc caagtaagcc gtgccgcgcg atcatttgac cgtggccgat gtgctcggca
     4561 tgcacaccgt gctgatgcag cgttacggcg gcgcaccggg tgtgcgtgat ccgggtgcgc
     4621 tggaggccgc cttgttccgc ccgcagaccg gctactacga cgacatcgtg gctgaagcgg
     4681 ccgcgctgct ggaaagcttg gcaatcaacc atccattcgt ggatggcaac aaacgcatcg
     4741 ccttcgccgc tgccgacgtg tttctgcgca tcaatggctg gcgcttgcat cgtgctccga
     4801 tgcagattca tgccgaggtg atcgagatgt tcgagaccag aacattcgac attgcccacc
     4861 tcgatccctg gctgcggtct ttcgccaccc ttgcagagtg atgccacgaa ggaaaaagca
     4921 ccccatatcc agacacaggc tgcgatggcc atccccccca caggttggct gacgccagaa
     4981 accgggtttt ctcgtaccct tacccgagga ggctccatga agaaatcccg tttcaccgac
     5041 agccagatca acgcctcatg cacgccaaag ccccgtaacc tcaggttccg gggccttttt
     5101 tgtcgtggct gcgtcgccag cccgcgcgca ggcaggcatg acagacagat gcgggcatga
     5161 aaaaagcggc ccgtgggccg ctcgtgcagg ctcttctggc ggagacggag ggattcgaac
     5221 cctcgatgag gctctacacc ccatactccc ttagcagggg agcaccttcg gccactcggt
     5281 cacgtctcca gaaggcaagc gcgcattatg ccaccatcag gcggcgctgg gctgatcgag
     5341 gtcgaacgcg cggtgcaggg cgcgcaccgc cagttccatg tatttctcgt cgatgacgac
     5401 cgaggtcttg atctcgctgg tggagatcat ctgaatgttg atgccttcct cgctcagcgc
     5461 gcggaacatc ttgctggcca cgccgacgtg gctgcgcatg ccgatgccga cgatgctgac
     5521 cttgcagatg cgggggtcac ccaccacgtc ggcggcgccc agcgccgggg ccaccttgtc
     5581 cttcagcagg tccatcgcgc gggcgaagtc gccgtggttg acggtgaacg acaggtcggt
     5641 cttgccgtcc ttgctgatgt tctgaatgat gacgtcgacc tcgatgttgg cgtcggccac
     5701 cgcacccagg atctggtagg cgatgcccgg cttgtcgggc acgcccaaca ccgaaatctt
     5761 ggcttcgtca cggttgaagg caatgccgga aacgacggct ttttccatgt gttcgtcttc
     5821 ctcgaaagtg atcagggtgc cggagcgggc ctcttcggcg aggtcgatgt cccagggcgt
     5881 gaagctggac agcacgcgca gcggcacttt gtacttgccg gcgaattcga ccgagcggat
     5941 ttgcagcacc ttgctgccca ggctggccat ttcgagcatt tcctcgaagc tcaggctggc
     6001 cagacggcgc gcctcgggca cgatgcgcgg atcggtggtg tagacgccat cgacgtcggt
     6061 gaagatcatg gcctcgtcgg ccttgagcgc ggccgccacg gcgacggcgg aggtgtcgct
     6121 gccgccacga cccagcgtgg tgatgtggcc gtcggcgtcc acgccctgaa agccggtgat
     6181 gatgaccacc ttgccggcgt ccaggtcggc gcgcacgcgc tggtcgtcga tgctgtcgat
     6241 gcgtgccttg gtgtaggcgt tgctggtgcg gatcggcacc tgccagccgg cgtagctgac
     6301 ggccggctgg ccctcggcct gcagcgcgat ggccagcaac gccgacgagg cctgctcgcc
     6361 ggtggcggcc agcatgtcct gctcgcggta atagtcctgc gtcggcttgc cgggggccag
     6421 ttccttggcc agcgccagca ggcggttggt ttcaccgctc atggcgctgg gcacgaccac
     6481 catccggtgg ccggcgcgcg cccatttggc cacgcgcttg gcgacctgcg caatgcgctc
     6541 ggtggagccc atcgaggttc caccgtactt ctgaacgatc aatgccatgg aatgcctgga
     6601 aaagacgaaa tcggtgcgcg ccagggctgg cgcggtaaac ccggcatttt agcgttcggc
     6661 gtccggcgcc cagtgcagca ccgcgccgcg acgcgacacc cggccggcgc ccaccttgag
     6721 ctggatgcgg tgaccacgcg tgcgcgccgc cgccacctgc gccagcagcg ccgccagttg
     6781 cgcgctgctg ggcgtctggg cgtgcgtgct ggccagccag tggcgcagca ggttggcctg
     6841 gcgcggcgcg gacagcgtct gcagtgcgtc cagacgcggc ggcacgccgc agtgcgccag
     6901 gtcctgtgcc gccacgtcgg caaggatctg ctgcgcctcg gcggcgtggg cggcgctgcg
     6961 ggcgaaggtg gtgcgatagg ccggccagca ggcctgcagc gccggcagca ggcgcgcgcg
     7021 gatgcggttg cgggtgtagc gctcgtcggc gttgctcggg tcctcgaccc agccctggcc
     7081 gcgcgcgcgc agccaggcgc gaatggccgc cgccggcacg cccagcagcg ggcgccggta
     7141 cgccacgtcg ccgcgctgcc agcgcgccgg catggccgac aggccgggca gcccggcgcc
     7201 gcgcgacagc gccagcagga tggtttcgac ctggtcgtcc gcatgctgcg cgatgactac
     7261 tgttttgata gctgcttgcg cttgactggc aagcgccaga gcatcaaatg cctcgtaacg
     7321 atggcggcgc gccgcgtcct ccgggctctg gccgcgggcc ggccgcgcat cgacacggcg
     7381 ccgcagcagg ggcacgtcca ggcgcgcaca aagcgcgctg cagtggcgct cgaagtcgtc
     7441 ggccgccggc tgcaggccgt ggtgcacatg ccaggcctgc acctgccccg gccagcgcgc
     7501 cgcgcaggcc agcagcagcg cggtcgaatc ggcgccgccg ctcagggcca ccgccagcgg
     7561 cagcgcgggc gcctcggccg cgaaggcgtc catggcggcg tcgacgcggg ccggatcggc
     7621 gtcgctggcg cgaagtgacg aggtgggacg ggacatgccg ggcgggtgcc gcaccagagg
     7681 gtgggcctgg ccgtgatggt atcggccccg cacccgccgc tgcggcggcg cccaaaacaa
     7741 aaggcacccg gacttgcgtt cgggtgcctt gcagggttgt ggtaggcgcg attggattcg
     7801 aaccaacgac ccccaccatg tcaaggtggt gctctaacca actgagctac gcgcctgaaa
     7861 atcgattcga agcccgcaag tatagcgaat tccggcgtca gcgccgtttg gccacgcgca
     7921 cgccgggcac gtcctgcacc tggcgcagca cgaaggccag gcgctgggtg tcgctgacct
     7981 cgaccgtgaa ggtcatccag gcccagccct tgacggactg cgtctgcacg ccgatgacgt
     8041 tcatcttctc gcgcgcgaag acatccgaga tgtcgcgcag caggccctgg cggtccagcg
     8101 cctccaccgc cacgtcgacc ggataggccg gcaggtggcc ggtggccggc gtgctccagc
     8161 cgacttcgat cacgcgctcg ggctcgcgcg cctgcaggtc gcgaaagctg gcgcagtcgc
     8221 gccggtggat ggtgacgccc cgcccgcgcg tgacgtagcc ggcgatgtcg tcgggcggcg
     8281 ccggcttgca gcagcgcgcc ggctgcgtca gcagcgaatc cagcccgacc accagcacgc
     8341 caccgcgacc gcctgcgctg ccggcgcgcg ccttgcgcag cagcacctgg tcatcgggcg
     8401 ccggcgtcgg ctcgggcggg cgcagcacgg cttcgatggc gcgcagcgac agctcgtcct
     8461 tgcccacggt ctcgaacagc gcctcgggcg tcttgaaacc cagttcggcc gccaggtgtt
     8521 ccagcgacag cgccgtgcgg ccttcgcgct gcagcagctt ttcgaccatc tcgcggccgc
     8581 gcgccacggt ttcgcgcgtg tgctgggcgt tgaaccaggc gcgcaccttg gccctggcgc
     8641 gcgaggacgc caggtagcgc agctcggggt tgagccagtc gcgcgagggc ccgccttcct
     8701 tggcggcgac gatctccacc gtctggccgt tcttcagcgg cgtgtgcagc ggcagcatgg
     8761 cgccgtcgac gcgcgcgccg cggcagcggt ggcccagctc ggtgtgcagc gtgtaggcaa
     8821 agtccaccgg cgtggcgccg gccggcagct cgacgatggc cgcctcgggc gtcagcacgt
     8881 agatgcggtc gtcgaacagg ccgtgctgcg gcgcggcgcc ggacagctcg cgctcccagg
     8941 ccagcagctg gcgcagcacg gcgatcttgg cgtcgtattc gccggccgcg ctgacgccgg
     9001 cgtagccctt ggtgccggcc tccttgtagg cccagtgggc cgccacgccg tgttcggcgt
     9061 gctggtgcat gtcgtgggtg cggatctgga tttcccaggc gcggccgtcg gggtcgcgca
     9121 ccacggtgtg cagcgagcgg tagccgttgg gcttgggacg cgcgatgtag tcgtcgaact
     9181 cctccggcac cggcgtgaag tgctgatgca cccagcccag cgcggtgtag cagtcgacca
     9241 cggtgggcac caggatgcgc agcgcgcgca cgtcgaacac ctgttcgaag ccgagcgact
     9301 tgccgcgcat cttgcgcacg atgctgctga tgtgcttggg tcggccgctg acgctggcgt
     9361 cgatgccctg ggcgcgcagc gccgcttcga tggcggcgcg gcgctgctcc agcgccagtt
     9421 cgcgctcgac gcgcttttcg tccagcaggc tggcgatgtg acggtaggtg tcgggttcct
     9481 gggcgcggaa caccaggtct tccatttccc acttgatctg ccagatgccc aggcggttgg
     9541 ccagcggggc gaacacctcg tgcgattcac gcagcagacc gggcggcggc gggcggcgcg
     9601 cggcggcgta gtagcgcagc gtctgcaggc gcgaagccag gcgcagcagc accacgcgca
     9661 gatcgcgcga aaaggccagc agcatcttgc gcacgaactc ggtctgctgg ctggcctgct
     9721 cggtgtcggc cgacagctgc tggcggctgg cgcgcgagcg ctgctgcaac tgctccagcc
     9781 gcgcggtttc gagcgccagg cgcgccaggc tgtcgccgaa ggccttgccg atcacctcct
     9841 ccggccggtt cagttgcgcg ctggcgtagc tcaggtaggc cgccgcctgg atcgcctcgg
     9901 agccgccaat gcccgccagg atgtcggcca ccgcgtcggc gtgggtcagc acgttctcgc
     9961 ccgaggccgt ggtctcgccc gccagcagcg gctcggcgaa ggcgcgcgcg cgtgcgcgca
    10021 gctcacccgc ctcgggctgg ccgccgccgg tggctaccac cacgtcggcc accgactggc
    10081 gcgtgccggc aggcgccacc gacagtgtct tcatggcgct tcgcccgccg gcagcaggaa
    10141 gtcgagcacg gccgccacct ggtcgtcggc caccagcgtg ggcgcgtgac ctacgccggc
    10201 aaattcgagc aggcgcgggc gcgggccgcg cgcctgcatg gcgctggccg tggcggccga
    10261 cagcaggtcg gattcggcgc cgcgcagcag cagggtgtcg gccttgaccc ggtcgtacag
    10321 ctgccacagc agggcctcac cctgggccga cgactcgggt gtgacggcgg cgtagggcac
    10381 ggcgatggcc gggtcgtagt gcaggcgcca gtcgccttcg ggccgggcgc gcagcatggg
    10441 gcgcgacagc gccagccatt gggcgtcggt gtgcgggccg aagccgggcg ccagcgcgcg
    10501 caggcatgcc gccccggcct cgaccgactc gaacggaccg ctcttgccca ggtactggcc
    10561 gatgcgctgc agcgcctgcc attcgatcac cggaccaacg tcgttgagca ccagccggcg
    10621 cagcggcgcc ggcagcggca gatcgggcgc gccggccagc atcaggccga tcagtccgcc
    10681 catgctggtg ccgacccagt cgaagcgccc gatcggcgcc tgctgcgcca gcgtccccag
    10741 cagggtcagc atgtcgccca cgtaggtggg gaactggtag cccatgggct ccttcagcca
    10801 gtcgctctcg ccgcgcccga ccacgtccgg gcagaccacg cgcaggggct ggccgcgcgc
    10861 cgccgcgcgc gccagcaggg cgcgcgccag cacgtcgaaa tcgcgcccct ggcgcgacag
    10921 gccatgcacg cacaccacca ggtgcgcggc cgccgcatcg ccccactgcc agcaggccat
    10981 gcggtggcca ccgctggcgt cgggacagga aacatggtgc aggacgggtt cggtcatggc
    11041 ggcgggtcgc tcggctggcg ccccggcggc gacggccggg gcagggtcgc aatcatggta
    11101 aatcaactgg cccccagggg gcgcaccggg gtggcccgca gaagacctgg tgaactatca
    11161 aaacgatagc ttctggcgct gtatgggtaa gcgctggcgg ccaatttgac tggtaatcga
    11221 cgtcaatgcg cctgcaccgt gcccgagacc gtcacctgca ccaggctgcg ccccggctcc
    11281 accggcaccg gagcgtcggc ggccatggcc tccatcctgc tggccatgcc gaacccgcgt
    11341 ggcgcgatgt cgcccgcgtc cccgctgacg ctgacctcgc gcagcgtgta gccggcaaag
    11401 ccgaaggcgc gcgccacgtc ggacgcccgc gccttgaagc gttcgatcgc ctgcgtctgc
    11461 gcctcggttt cggcacgggc gcgctgggtg cggctcaggc caaaggcaag ctggtcgatc
    11521 accatcgtgg tggcgcgcgc ggccgcctgc gtgatgcggg cgaagtcgcg cccttccagc
    11581 accagctcgg cacggccctg ccagccgctg atgcggccgt tgcggtcatg gcgcggatgc
    11641 acacggaagg cgccggtgcg cacctccatc tgatcggccg gcgcgctggc gccgacctgc
    11701 cgcagcgcct cgtccagcgc ctgcctcagc gcggcctgca cgccggcggc gtcggcgcct
    11761 tcgcgcgcgg tgccgagcgt cagggtgagc agatcctgca ccacctcgac ctggccactg
    11821 gcgctgagct gaatcacgtt ctgtggcacg ttcggggtcg gactcatggg tgtggcgggg
    11881 ctgtgggtct gggcatgggc tgcgggcgcg gcgatggcca gcagcagggc cagcgcgcgg
    11941 gcggcgcgta caagtcgatg gttcatggga taaccgcagt cggcgcgccc ggcggcgcgg
    12001 ggcgccatga tggcacaggg ttcagcggga cccgagttgg gtgacttcgg ccacgccgtg
    12061 gcgctggccg cgggcagcgg cgcgacgcac ctgctcgcgc agctgctggc gctgctgggc
    12121 gtccagacga cgatcgggca ccgggccgac ctcgcgctgg cggaacatcg actccaccgc
    12181 ctgccacagc gggctgcgcg ccagtgccgc ctcggacacc gcgggcggtg cgccgctggg
    12241 gccggattcc gacgcttcgc ccacggcctg cgcgcgcacg gccatcggca gcgccagcgc
    12301 gccccagatt ccaagcaaac agatccagcg gttcatgtgc atatcaaccg ctgaccatcc
    12361 cacaccgatg acgtgagcgt ccggcagata gctgccccgt ggggcaacag gtgtaacact
    12421 gtgaaaagca gcccgtggaa gccctgaatt cctcacgctt tcggggtcaa aatgggcctg
    12481 ttacaaattc gctgaaaggt gctggatgaa ccagtctgcc aaccgaaccg acaagatcgt
    12541 catcgtcgac gacgatgccc gcatccgcga tctgctgcgg cgctacctgg cgcaggaagg
    12601 cttcgaagtc atcgtggccg aggacgccaa ggcgctggcg cgcgtgatgc tgcgcgacac
    12661 catcgacctg atcgtgctcg acctgatgat gccgggcgaa gacggcctgt cggtgtgccg
    12721 gcgcctgcgc gcggcgggcg acaagacgcc catcatcatg ctcaccgcca agggcgagga
    12781 cgtggaccgc atcgtcggcc tcgaagtcgg cgccgacgac tacctgggca agccgttcaa
    12841 cccgcgcgag ctgctggcac gcatccacgc cgtgctgcgc cgccgtccgc cgctggaagc
    12901 gccgggggca ccctcggccg agaacgagtc ggtcgccttc gggccgttcc agttcgacct
    12961 gggcgcgcgc accctgcaca agaacggcga ggaagtgccg ctgaccaccg gcgaattcgc
    13021 catgctcaag gcgctggtgc gccacccgcg ccagccgctg tcgcgcgaga agctggcgca
    13081 gctggcacgc gggcgagagt tcgagccctt cgaccgcagc ctggacgtgc aggtgtcgcg
    13141 cctgcgcaag ctgatcgaac aggacgccgc cagcccacgc tacatccaga ccgtgtgggg
    13201 cgtgggctac gtgttcgtgc ccgacggcac cggctgacca ccgtgcccga gcgcgccttc
    13261 ggcgtgtccg cgggttcgac gctgccgccg acggacatcg agccgccgac cacgcgcccg
    13321 gacctgctgc ccgactccag caacgcgccg ctggagccgg aggtcgaggc gcgcgcgcca
    13381 ctggccttca gcctgttctg gcgcaccttc tttctgctgg cgctgctgct gctgggcacc
    13441 acggtcggct ggtaccagat gttccgagcg ctcgagtacg agccacgcgt gatcggcaac
    13501 gcgcgccaga tcgcctcgct ggtcaacctc tcgcgcgcgg cgctgatcca ctccgacgcc
    13561 atcgcccgcg tgtcgctcat caagacgctg gccgagcagg aaaaggtgcg catcgcgccg
    13621 cgcgagccgg gcgaccgctt cgagctgttc aaccagaccg tgctggagcg ccgcatgagc
    13681 ggcgagatcg tggcccgcct gggcctgggc acggtggtgg ccagccgggt caacggggaa
    13741 gaggggctgt gggtcggctt ttcgatcgag ggcgactcgt actggctgct gatggaccgc
    13801 tcgcgcatcg gcaccctgct cagcggcagc gcctggctgc tgtggctggc cacgctgggc
    13861 gccgcctcgc tgctgggcgc ggcgctgctg gccggctaca tcaaccggcc gctgcgccag
    13921 ctgtcgaatg ccgccgcccg cgtgcgcgag ggcgactacc tggaaggccg gctcgacgaa
    13981 accacgcgcg cgcgcgagat ccgcgccgtc aacatcggct tcaaccgcat ggccgaggag
    14041 ctgtcgcgca tcgaacagga gcgcgccgag atgctggccg gcatctcgca cgacctgcgc
    14101 acaccgctgg cgcgcctgcg cctggaaacc gaaatgagcg tgcccgacgc cgaggcgcgc
    14161 gagcacatgg tggccgacat cgcccaggtc gacaccatca tcgacaagtt cctcgactac
    14221 gcccggcccg accacgtgag tctgcaggcg ctgccgctgg cggcgctggc gcaagccgcg
    14281 gcccagccgt ttgccagccg cgaggacatg caggtgcaga tcgacgtgcc gcccgagctg
    14341 cgcgtgatgg gcgacgaggt ggagctgtcg cgcgtgctgg ccaacctgct ggaaaacgcg
    14401 cgccgctacg gcaagagcgc cgacagcggc atcgcccgcg tgcgcatcgc cgccaccgcg
    14461 cgcgaccagt gggtgacgct gcgcgtacgc gaccacggcc ccggcgtgcc cgaggagcag
    14521 ctggccgtgc tcacgcgccc gttcttccgt ggcgacaccg cccgcaccga agccaccggc
    14581 gccggcctgg gcctgtccat cgtcgccaag atggtgcgcc acatgggcgg tgaactgaac
    14641 ctgaccaaca gccccagtgg cggcctgatg gcggtgatcc ggctgccgca ggcgtccgac
    14701 aggcgcgccg accatggctc cctgcgcaag cgccgctgat tcagcctgga aacggcagtt
    14761 ggacgcgccc gcccgtgccg cgatcggcgt gccaggcaag acgcgacgcc gccgcgggct
    14821 cgtgcccgca agcaggagca acgccgcatg gcgcggcgag cgcggtgcgg gtgggtgcgc
    14881 agcgcgctca cccaggaggt gaaggccatc gcagccgcca gaccccgcat cgatgcggtc
//
LOCUS       DAOQTZ010000069        14638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4639_length_14638_cov_13.253377, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000069 DAOQTZ010000000
VERSION     DAOQTZ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14638
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4639_length_14638_cov_13.253377"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3182
                     /gene="smc"
                     /locus_tag="PKN51_09125"
     CDS             <1..3182
                     /gene="smc"
                     /locus_tag="PKN51_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNT85299.1"
                     /translation="SYYINNQPVRRRDVQDVFLGTGLGPRAYAIIGQGTISRIIESRP
                     EELRLFLEEAAGVSKYKERRRETENRLHDTRENLTRVEDILRELNHNLDKLEKQAEVA
                     QQYQNLQAAAQLKQNQLWFLKRAEADEGQGRLRQEADKAVNDLESRLADLRHVEAELE
                     TIRQAHYAAGDQVNQAQGLLYEASAEVGRLEAEIRFVVEGRQRAEARLLQLKEQLGQW
                     AERRSQAEADIEQLAVQGVDAEDRAITLSAQLEEQQFTLPDLQEALRAAQAGAAEQRT
                     AVAQVQQQIQVLAAEQRGIEEQTRQLQARRERLAADRNALAAPDEQRLLNLQQQLSAA
                     QDGAEVAQARLAELQDSVPRLDDERRAAQQAVNTEAARQADLSARLDALRALQDKLKT
                     DGKLQPWLHKHGLDGLPGLWSRLHVEPGWEAALEAALRERMSALEVSRLDMVRAFAQD
                     APPAKLAFYSPPAAALPEGAAGLPRLADLLRLHDAGQKALLADWLAGCYTADSLEAAL
                     AQRAQLKPGEAIYVASGHAVGAHGVSFYAPDSEQAGLLARAQEIENLDKQLRAQQLIS
                     DEARSALVRAEAAYSAAAEQLTGSRRDAAEAQQRAHELQVETLRLSQLAEQTRARSEQ
                     IGTDLAEVDAGLEALQERRITAEARFEELDMQLADTQERHAGLDQRVIDAEQRLNQAR
                     EQQRTLERELQEAQFAQRSLQARRDELTRAIDTAQQQGAAIEAEQQRASEELERLTDA
                     AAQAGLQAALGLKLEREQALAAQRSSYDDLTARLRASDERRLRLERELDPLRQRITDL
                     QLKEQAARLGTEQYTQLLADAQADLQAVARSIEEGSVRLNGLQGEIDRLHQGIQALGA
                     VNLAALDELTTARERKQFLDAQSADLTEAMTTLEDAIKKIDAETRELLGGTFNTVNEH
                     FGRMFPELFGGGQAKLVMTGEEILDSGVQVMAQPPGKKNQTIHLLSGGEKALTAIALV
                     FAIFQLNPAPFCLLDEVDAPLDDANTERYAKLVTRMSGQGTQFLFISHNKIAMEMAEQ
                     LIGVTMQEQGVSRIVAVDMESAVGMAEAS"
     gene            3268..4335
                     /locus_tag="PKN51_09130"
     CDS             3268..4335
                     /locus_tag="PKN51_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNT85300.1"
                     /translation="MSSLQVGLAVAGGLILGGVIAYNTWNARRHQPRQAQPEAPAPAE
                     RVEPGLQEAMAAPERQEPSFDAEAALPSLGPLPLPDKRPRLDALIDVIAPIALDGRVV
                     SGDALLAALPATRRVGSKPFAVEGQHDTRGEWEFPQAGQRYHAVQAGVQLANRTGPLN
                     DIEFSEFVMCAQRYADAVGGAPEFPDMLHEVARARELDQFASAHDAQLGFTLRARGAA
                     WSPGFVQQQAARQGFVAGAIPGRMVLPAAEPGLPPLLDLSFDAQAALSEDVAHAAITA
                     LVLNLDVPQVHRAERPFDRLRDIAFALAEAMDGVITDDAGQPLRPELIDGIGADLDRL
                     YDTLEQHDLAAGSPQARRLFS"
     gene            4385..6610
                     /gene="ligA"
                     /locus_tag="PKN51_09135"
     CDS             4385..6610
                     /gene="ligA"
                     /locus_tag="PKN51_09135"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNT85301.1"
                     /translation="MNNEANVSRRAAELRARLNRAAHQYYTLDAPELPDAEYDRLFRE
                     LQAIEAEHPALRTPDSPTQRVGGAVLDTLAPVRHAVPMLSIRTETDTSPQGAATFDQR
                     VRDYLASEQRRTLLKLDDERHRSQPLGPAAQAVLAGAPIDYVAEPKFDGLAMSLRYER
                     GVLVQAATRGDGETGEDVTHNIRTIGQIPLRLVAPTLAAARAALPPEGAGFALGRPGG
                     ETAAGVPPLLEVRGEVYMRRDDFERLNEVMRQRMAAGDKAAKTFVNPRNTAAGAVRQL
                     DAALAAERRLSFFAYGLGEVTAPADGGPRFDTHWAVLQALKSWGFPVSALARQARGAS
                     ELIAFHADVAAQRDALPFDIDGVVYKVDSLELQRELGFVTREPRWAVAHKYPAQEELT
                     TVQAIDVQVGRTGKLTPVAKLAPVFVGGVTVTNATLHNELEARRKDVRVGDTVVVRRA
                     GDVIPEVVSVLADKRPPGTAEFTMPASCPVCGSAVVREEGEADHRCTGGLFCPAQRKQ
                     ALLHFASRKAMDIEGLGDKLVDQLVDGNVVRSLPDLYRMGLVSLSALDRMAEKSAQNL
                     LEALERSKDTTLARFLFALGIRHVGEATAKDLARHFGTLDTLMDATPEQLAQVSDVGP
                     VVAESIHTFFAQPHNREVVEQLRAAGLHWSEATPAPGATLPLAGKTVVLTGTLPTLTR
                     EQAQALLEAAGAKVAGSVSKKTSYVIAGAEAGSKLDKARALGVPVLDEAGLQALLAGG
                     A"
     gene            6624..7556
                     /locus_tag="PKN51_09140"
     CDS             6624..7556
                     /locus_tag="PKN51_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNT85302.1"
                     /translation="MRNARGAAVSGLRLGIDLGGTKIEIAALDAQGGFALRQRVATPQ
                     GDYGATLRAIADLVAQAEAQLGQRGLPLGLGIPGSLSPLTGRVRNANSTALNGQPLRE
                     DLQHLLGRPVAVDNDANCLALSEATDGAGAGAEVVFAVILGTGVGAGIAVHGQVLHGC
                     NGVAGEWGHNPLPRAGAPELALRCWCGRTACQELFLSGPGLAADHARAGGGPAAAAAI
                     VAAAQAGDAAAQASLARYVERLARALAQVINLLDPDVIVLGGGMSNVQALYAELPRLW
                     GPQVFSDVVRTRLVAARHGDSSGVRGAAWLAAAG"
     gene            7702..8172
                     /locus_tag="PKN51_09145"
     CDS             7702..8172
                     /locus_tag="PKN51_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85303.1"
                     /translation="MVGWHAVRVPRAWGAGALAATLALLGGCVAVPVEPYEPGAAVAY
                     PGYETTVVTPGYYSAPAPYYYGAPSVSLGVWGWSGGSRHWHDRPPRRHDWGRPPPPHH
                     DWGRPPPPRPHWGGRPDGPRPDVRPSPRPPHGQWQRPSPPRPRAPLINPEGMGG"
     gene            complement(8263..8862)
                     /locus_tag="PKN51_09150"
     CDS             complement(8263..8862)
                     /locus_tag="PKN51_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNT85304.1"
                     /translation="MERLLEHKGLTTRLSDSIHQATDRATDLIRNAMGALGVPYRRGG
                     TSAETGFDCSGFVRSMYQQAVGLILPRTAAEQAASTAQITKTDLQPGDLVFFNTLRRA
                     YSHVGIYLGDGKFIHAPRAGAQVRVESMDISYWQKRFNGARRVLTRDSAATEAPAALP
                     RVTERQWPAPSAVRLGGGSGKYALPAPAEIEQPQSGDRI"
     gene            9273..9899
                     /locus_tag="PKN51_09155"
     CDS             9273..9899
                     /locus_tag="PKN51_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HNT85305.1"
                     /translation="MDEQLRIVVVAPDLAPSNPADERAMRQAERSRALRIGLLQHGFN
                     LVAVLPADALLEGHLTALQPDLVIVDAESEARDTLEHVVLATREAPRPIVLFTDDEDT
                     THVKDAMAAGVTAYVVAGLAPQRIRPILEVALARFAHEQALRTELVQARTALHERKLI
                     DRAKGLLMQRQSIAEQQAYDRLRRAAMDRGLRVAEVAQRMLDAADLLG"
     gene            10089..11378
                     /locus_tag="PKN51_09160"
     CDS             10089..11378
                     /locus_tag="PKN51_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT85306.1"
                     /translation="MTELPTTAAVTTTAAAPATGLPRRQLLQATAVGAVGISPALRAL
                     VHAQGSDAPEKKEVKIGFIPLTDCASVVMASVLGFDKKYGVTITPTKEASWAGVRDKL
                     VNGELDFAHVLYGLVYGVHLGTAGPKKDMAVLMNLNHNGQAITLSKALADKGAVDGPS
                     LAALMARDKREYTFAGTFPTGTHAMWMHYWLAAAGINPLSGAKLITVPPPQMVANMRV
                     GNMDGFCVGEPWNQRAIMDGIGITANTTQDIWRDHPEKVLGTTADFVKKYPNTTRAVM
                     MAVLEASRWIDASLSNKTRMAETIAAKSYVNTGVDAINQRILGRYQNGLGKSWDDPNH
                     MKFFGDGAVNFPYLSDGMWFLTQHKRWGLLKSHPDYLAVAKQVNQVELYRSVASAMKI
                     SVPKDAMRSSKLIDGVVWDGKNPAQYADGFKIKAQEA"
     gene            11405..12340
                     /gene="ntrB"
                     /locus_tag="PKN51_09165"
     CDS             11405..12340
                     /gene="ntrB"
                     /locus_tag="PKN51_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HNT85307.1"
                     /translation="MVSAVFHAPLDSSADNTKTIAASALSTGAAAQKDAKPANPPAAR
                     PARDWGALSRALWLAVLPPVLGVALLVGLWALVASSTGGSIPSPHDTWLQAQEVFSDP
                     FYRNGPNDQGVGWNVLASLQRVAVGFGLAALVGIPAGFAIGRFAFLARMFNPLISLLR
                     PVSPLAWLPIGLLVFKGANPAAIWTIFICSIWPMVINTAVGVQRVPQDYLNVARVLNL
                     SEWKVLTKILFPSVLPYMLTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNV
                     KNIIIAIFVIGIVGLLLELALVKLATAFTFEEVKT"
     gene            12450..13262
                     /locus_tag="PKN51_09170"
     CDS             12450..13262
                     /locus_tag="PKN51_09170"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85308.1"
                     /translation="MNASLTTKYIEIQGVEQTFRTAKGVFPALRDVNLTIAKGEFVAL
                     IGHSGCGKSTLLNLIAGLTSPTGGALLCANKEIKGPGPERAVVFQNHSLLPWLTCEDN
                     IYLAVERVFGARETKAQLKLRTAAALALVGLTHAGQKRPGEISGGMKQRVGIARALSM
                     EPQVLLMDEPFGALDALTRAKLQDELLDIVARTHSTVVMVTHDVDEAVLLSDKIVMLT
                     NGPAATIGEVLQVDLPRPRSRVELAEDARYQQYRKAVIDFLYTRQGHVEKAA"
     gene            complement(13374..>14638)
                     /locus_tag="PKN51_09175"
     CDS             complement(13374..>14638)
                     /locus_tag="PKN51_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNT85309.1"
                     /translation="PSLFPYDGPEAIWNEHRESTRGRDLDITGLTWAQLEAAPRQWPC
                     PEGEKHGRARLYTDRQFASADGRARFALQLPGTVAEPTDARHPFALITGRLRDQWHGM
                     SRSGLVESLWAHASEPRADMHAADMARLGLKDGDLAYLTSRRGSIVVPVQASGTLAPG
                     QVFLPMHWGDEALGGASSTGRRLAGVNALTSGARCPQSLQPELKHAAIKVLKAELPWG
                     LWAQAWLPLAQVQRVRQQLEALMDEFPFASCSLFGRAEVGSGVQLRAAAHQAVDEALI
                     ERIAGLLGLQGAGLLAYRDARRGQWRRARLEPAGKGQQLHAMLLAGDVSAQAWIAPLL
                     QECHDAQPLRRALLQPGAEPPQPLAPRGATVCACEGVGENAITGLLKACAGDAGARLA
                     RLKEELRCGTQCGSCVPQLQRMVQASMA"
BASE COUNT         2313 a         5133 c         5149 g         2043 t
ORIGIN      
        1 gcagctacta catcaacaac cagccggtgc gccggcgcga cgtgcaggac gtgtttctgg
       61 gcaccggtct gggcccgcgc gcctacgcca tcatcggcca gggcacgatc agccgcatca
      121 tcgagtcgcg acccgaagag ctgcgcctgt ttctggagga ggccgcgggc gtttccaagt
      181 acaaggagcg ccggcgcgaa accgaaaacc gtctgcacga cacgcgcgag aacctcacgc
      241 gggtggaaga catcctgcgc gaactcaacc acaacctcga caagctggaa aagcaggccg
      301 aggtggcgca gcagtaccag aacctgcagg cggcggccca gctcaaacag aaccagctgt
      361 ggtttctcaa gcgcgccgag gccgacgagg gccagggccg gctcaggcag gaggccgaca
      421 aggcggtcaa cgacctggag tcgcgcctgg ccgatctgcg ccatgtcgaa gccgagctgg
      481 aaaccatccg ccaggcgcat tacgccgccg gcgatcaggt caaccaggcg cagggcctgc
      541 tgtacgaagc cagcgccgag gtcggccggc tggaggccga gatccgcttc gtcgtcgaag
      601 gccgccagcg cgccgaagcg cgcctgctgc aactgaagga gcaactcggc cagtgggccg
      661 agcggcgcag ccaggccgag gccgacatcg agcaactggc ggtccagggc gtcgacgccg
      721 aagaccgtgc catcacgctc tcggcgcaac tggaggagca gcagttcacg ctgcccgacc
      781 tgcaggaggc gctgcgtgcc gcccaggccg gcgccgccga gcagcgcacc gccgtcgccc
      841 aggtgcagca gcagatccag gtgctggccg ccgagcagcg cggcatcgaa gagcagacgc
      901 gccagctgca ggcgcggcgc gagcgcctgg cggccgaccg caacgccctg gccgcgcccg
      961 acgagcagcg cctgctgaac ctgcagcagc agctgtcggc ggcgcaggac ggcgccgaag
     1021 tcgcccaggc gcggctggcc gagctgcagg acagcgtgcc gcggctcgac gacgaacgcc
     1081 gggcagccca gcaggcggtc aacaccgaag cggcgcgcca ggccgatctg tcggcgcggc
     1141 tggacgcgct gcgcgcgctg caggacaagc tcaagaccga cggcaagctg caaccctggc
     1201 tgcacaagca cgggctcgac ggtctgccgg ggctgtggag ccgcctgcac gtcgagccgg
     1261 gctgggaagc ggcgctggaa gcggcgctgc gcgaacgcat gtcggcgctc gaagtcagcc
     1321 gcctggacat ggtgcgcgcc ttcgcccagg acgcgccgcc ggccaagctg gccttctaca
     1381 gtccgccggc cgccgcgctg cccgaaggcg cggccggcct gccgcgtctg gccgacctgc
     1441 tgcgtctgca cgacgccggg cagaaagccc tgctggccga ctggctggcc ggctgctaca
     1501 ccgccgacag cctggaagcc gcgctggccc agcgcgctca gctcaagccg ggcgaggcca
     1561 tctatgtggc cagtggccac gccgtcggcg cgcatggcgt cagcttctac gcgcccgatt
     1621 ccgaacaggc cggcctgctg gcgcgcgcgc aggaaatcga gaacctcgac aaacagctgc
     1681 gcgcccagca gctcatcagc gatgaagcgc gcagcgccct ggtgcgggcc gaagccgctt
     1741 acagcgccgc cgccgaacag ctgaccggca gccgccgcga cgccgccgag gcgcagcaac
     1801 gcgcgcacga gctgcaggtc gaaaccctgc gcctgagcca gctggccgag cagacgcgcg
     1861 cccgcagcga gcagatcggc accgatctgg ccgaggtcga tgccggcctg gaagcgctgc
     1921 aggagcgccg catcaccgcc gaggcgcgct ttgaagagct ggacatgcag ctggccgaca
     1981 cgcaggagcg ccacgccggc ctggaccagc gcgtgatcga cgccgagcag cgcctgaacc
     2041 aggcgcgcga gcagcagcgc acgctggagc gcgagctgca ggaggcccag ttcgcccagc
     2101 gcagcctgca ggcgcgccgc gacgagctca cgcgcgccat cgacaccgcc cagcagcagg
     2161 gcgccgccat cgaggccgag cagcagcgcg ccagcgagga actggagcgc ctgaccgacg
     2221 ccgccgccca ggccggcctg caggccgcgc tgggcctgaa actcgagcgc gaacaggcgc
     2281 tggcggcgca gcgcagcagc tacgacgacc tgaccgcgcg cctgcgcgcc agcgacgaac
     2341 gccgtctgcg gctggaacgc gagctcgacc cgctgcgcca gcgcatcacc gacttgcagc
     2401 tcaaggagca ggcggcgcgc ctgggcaccg agcagtacac gcagctgctg gccgacgccc
     2461 aggccgacct gcaggcggtg gcgcgctcga tcgaggaagg cagcgtgcgc ctcaatggcc
     2521 tgcagggcga aatcgaccgc ctgcaccagg gcatccaggc gctgggcgca gtcaacctgg
     2581 cggcgctgga cgagctgacc acggcgcgcg agcgcaagca gttcctggac gcgcagtcgg
     2641 ccgacctgac cgaggccatg accacgctgg aagacgccat caagaagatc gacgctgaaa
     2701 cgcgcgagct gctgggcggc accttcaaca ccgtcaacga gcacttcggt cgcatgttcc
     2761 ccgagctgtt cggcggcggc caggcgaagc tggtgatgac cggcgaggaa atcctcgact
     2821 ccggcgtgca ggtgatggcg caaccacccg gcaagaaaaa ccagaccatc cacctgctgt
     2881 cgggcggcga aaaggcgctc acggccatcg ccctggtgtt tgccatcttc cagctcaacc
     2941 cggcgccgtt ctgcctgctc gacgaagtcg acgcgccgct ggacgacgcc aacaccgagc
     3001 gctacgccaa gctggtcacg cgcatgagcg ggcagggcac gcagttcctg ttcatcagcc
     3061 acaacaagat cgccatggaa atggccgagc agctgatcgg cgtcaccatg caggagcagg
     3121 gcgtctcgcg catcgtggcg gtggacatgg aaagcgccgt cgggatggcc gaagcatcat
     3181 gagcgcgcct gtgcgtcggc cggccccgcg gcccctgacc aggcgggtcc tgcccggcgt
     3241 gcgcggcgcc cgaggagctg tgtcgacatg agttctctgc aagtgggcct ggcggtggcc
     3301 ggtggcctga tcctgggcgg cgtgatcgcc tacaacacct ggaacgcgcg tcgccaccag
     3361 ccgcgccagg cgcagcccga agcgccggcg cccgccgagc gcgtggagcc cgggctgcag
     3421 gaggccatgg cggcgcccga gcgccaggag cccagcttcg acgccgaagc cgccctgcct
     3481 tcgctcggcc ccttgccgct gcccgacaag cgtccgcggc tggatgcgct gatcgacgtc
     3541 atcgccccca tcgcgctgga cggccgggtg gtgtcgggcg acgccttgct ggcggcgctg
     3601 ccggccacgc gccgtgtggg cagcaagccg ttcgcggtcg aaggccagca cgacacccgt
     3661 ggcgagtggg agtttcccca ggccggccag cgctaccacg cggtgcaggc gggggtgcag
     3721 ctggccaacc gcaccggccc gctcaacgac atcgagttct ccgaattcgt catgtgtgcg
     3781 cagcgctacg ccgatgccgt gggtggtgcg ccggaatttc ccgacatgct gcacgaagtg
     3841 gcccgcgcgc gcgaactcga ccagttcgcc agcgcccatg acgcgcagct gggctttacc
     3901 ctgcgcgcgc gcggcgccgc ctggagcccc ggtttcgtgc agcagcaggc ggcgcgccag
     3961 ggcttcgtgg ccggcgccat ccccggccgc atggtgctgc ccgccgccga gccggggctg
     4021 ccgccgctgc tggacctgag tttcgacgcc caggccgcgc tgtccgagga cgtggcgcac
     4081 gccgccatca cggcgctggt gctcaacctg gacgtgccgc aggtgcaccg ggccgagcgc
     4141 ccgttcgacc gcctgcgcga catcgccttc gcgctggccg aggccatgga cggggtcatc
     4201 accgacgacg ccggccagcc gctgcggccc gagctgatcg atggcatcgg cgccgacctg
     4261 gaccgcctgt acgacacgct ggagcagcac gacctggcgg ccggctcgcc gcaggcgcgc
     4321 cggctgttca gctgatttca gattgttttg gcctccagcg cccgcccaga gtgcgcgagc
     4381 agctatgaat aacgaagcaa acgtgagccg acgagcggcc gagctgcggg cccggctgaa
     4441 ccgcgccgcc caccagtact acacgcttga cgcgcccgaa ctgcccgacg ccgaatacga
     4501 ccgcctgttt cgcgagctgc aggccatcga ggccgaacac cccgcgctgc gcacgcccga
     4561 ctcgcccacc cagcgcgtgg gcggtgccgt gctcgacacg ctggcaccgg tgcgccacgc
     4621 cgtgcccatg ctcagcatcc gcaccgaaac cgacacctcg ccccagggcg cggccacctt
     4681 cgatcagcgc gtgcgcgact atctggccag cgagcagcgg cgcaccctgc tcaagctcga
     4741 cgacgagcgc caccgcagcc agccgctggg cccggccgcg caggccgtgc tggccggcgc
     4801 gcccatcgac tacgtggccg aacccaagtt cgacggcctg gccatgagcc tgcgctacga
     4861 acgcggcgtg ctggtgcagg ccgccacgcg cggcgacggc gaaaccggcg aagacgtcac
     4921 gcacaacatt cgcaccatcg gccagatccc gctgcgtctg gtcgccccca cgcttgccgc
     4981 tgcgcgtgct gcgctgcccc ccgagggggc tggtttcgcc ttggggcggc ccggcggcga
     5041 aaccgctgcc ggcgtgccgc cgctgctcga agtgcgcggc gaggtctata tgcgccgcga
     5101 cgatttcgag cgcctgaacg aggtcatgcg ccagcgcatg gccgccggcg acaaggccgc
     5161 caagaccttc gtcaacccgc gcaacaccgc cgccggcgcg gtgcgccagc tcgatgccgc
     5221 gctggcggcc gagcggcggc tgagcttttt cgcctacggc ctgggcgagg tgacggcgcc
     5281 cgccgacggc ggccctcggt tcgacaccca ttgggccgtg ctgcaggccc tgaaatcatg
     5341 ggggtttccg gtctccgcgc ttgccagaca agcgcgaggc gcttcagagt tgatagcatt
     5401 tcacgccgat gtggcagccc agcgcgacgc gctgcccttc gacatcgacg gcgtggtcta
     5461 caaagtggat tcactcgaac tgcagcgcga gctgggcttc gtcacgcgcg agccgcgctg
     5521 ggcggtggcg cacaaatacc cggcgcagga agagctgacc accgtgcagg cgatcgacgt
     5581 gcaggtcggg cgcaccggca agctcacgcc ggtggccaag ctggcgccgg tgttcgtggg
     5641 cggcgtcacc gtcaccaacg ccaccctgca caacgagctg gaagcgcgcc gcaaggacgt
     5701 gcgcgtgggc gacaccgtgg tggtgcgccg cgccggcgac gtgattcccg aggtggtgtc
     5761 ggtgctcgcc gacaagcgcc cgccgggcac ggctgagttc accatgcccg ccagctgccc
     5821 ggtgtgcggc agcgcggtgg tgcgcgagga gggcgaggcc gaccaccgtt gcaccggcgg
     5881 cctgttctgc ccggcccagc gcaagcaggc gctgctgcac tttgccagcc gcaaggcgat
     5941 ggacatcgag ggcctgggcg acaagctggt cgatcagctg gtcgatggca acgtggtgcg
     6001 cagcctgccc gacctgtacc gcatgggtct ggtcagtctg tcggcgctgg atcggatggc
     6061 cgagaagtcc gcccagaacc tgctggaagc gctggaaaga tcgaaggaca ccacgctggc
     6121 gcgctttctg ttcgcgctcg gcatccgcca cgtgggcgag gccacggcca aggatctggc
     6181 acgccacttc ggcacgctgg acacgctgat ggacgccacg cccgagcagc tggcgcaggt
     6241 gagcgacgtc ggcccggtgg tggccgaaag catccacacc ttttttgccc agccgcacaa
     6301 ccgcgaggtg gttgagcagc tgcgcgccgc cgggctgcat tggagcgagg ccacgcccgc
     6361 acccggtgcc accctgccac tggccggcaa gacggtggtg ctcaccggca ccctgcccac
     6421 gctcacgcgc gagcaggccc aggccctgct cgaagccgcc ggcgccaagg tggccggcag
     6481 cgtcagcaaa aaaaccagct acgtcatcgc cggcgctgaa gccggcagca agctcgacaa
     6541 ggcgcgggcc ctgggcgtgc cggtgctgga cgaggccggc ctgcaggcgc tgctggcggg
     6601 cggggcatga acgtgccgcg ccgatgcgca acgcgcgggg tgctgcggtg agcgggctgc
     6661 gcctggggat cgatctgggc ggcaccaaga tcgagatcgc cgcgctggac gcgcaaggcg
     6721 gctttgcgct gcgccagcgc gtggccacgc cgcagggcga ctacggcgcc accctgcgcg
     6781 ccatcgccga tctggtggcg caagccgaag cccagctggg ccagcgcggc ctgccgctgg
     6841 gcctgggcat tccaggcagc ctgtcaccac tgacgggccg ggtgcgcaac gccaactcca
     6901 ccgcgctcaa cggccagccg ctgcgcgagg acctgcagca cctgctgggt cggccggtgg
     6961 cggtcgacaa cgacgccaac tgcctggccc tgtctgaagc caccgatggc gccggggcgg
     7021 gtgccgaggt ggtgtttgcg gtgattctgg gcaccggcgt gggtgccggc attgccgtgc
     7081 acggccaggt gctgcacggc tgcaacggcg tggccggcga atggggccac aacccgctgc
     7141 cacgcgcggg cgcccccgag ctggcgctgc gctgctggtg cgggcgcacc gcctgccagg
     7201 agctgtttct gtccggcccc ggtctggcgg ccgaccatgc ccgggctggt ggcgggccgg
     7261 ccgcagccgc cgccatcgtg gccgccgcgc aggccggcga tgcggcggcg caggccagcc
     7321 tggcgcgcta tgtcgagcgg ctggcgcgtg cgctggcgca ggtgatcaac ctgctcgatc
     7381 ccgacgtcat cgtgctgggt ggcggcatga gcaacgtcca ggcgctctac gccgagctgc
     7441 cgcgcctgtg ggggccgcag gtgttcagcg acgtggtgcg cacccggctg gtggcggcgc
     7501 gccatggcga ttcgtccggc gtgcgtggag cggcctggct ggcggccgcg ggttgacgga
     7561 taccggcggt ttcgattggc aggtcagccg gcgcggcagc gggaacgttg tggggccgaa
     7621 cggtgtctga acatcgtcgg acggctgccg ggccatggca cccttcggtg tcgccagccg
     7681 tcctgcaagg gagcaagagc tatggttggt tggcatgcgg tgcgggttcc ccgcgcctgg
     7741 ggcgcaggtg cgctggcggc cacgctggcg ctgctgggcg gctgtgtggc cgtaccggtc
     7801 gagccgtatg agcctggtgc ggcggtggcc tatcccggct acgaaaccac cgtggtcacg
     7861 cccggttact acagcgcgcc ggcgccgtac tactacggcg cgccgtcggt gtcgttgggg
     7921 gtgtgggggt ggtcgggcgg atcgcgccac tggcacgatc gaccgccacg gcgtcacgac
     7981 tggggccggc cgccgccacc gcatcacgac tggggcagac caccgcctcc gcgtccacat
     8041 tggggcggtc ggccggacgg tccgcgtccc gacgtcagac cgtcgccgcg cccgccccat
     8101 ggccagtggc agcggccgtc gccgccgcgc ccgcgcgcac cgctgatcaa ccccgaaggg
     8161 atgggtggct gagccctgcc gcctgcgctc ctggcgcgac caaaaaagag ggcccttgcg
     8221 ggcccctacc tttggaggaa ggtcaatcgg tcggtgtgcg gatcagatgc ggtcgccgct
     8281 ctgcggctgc tcgatttcgg ccggcgcggg cagggcgtac ttgccgctgc cgccacccag
     8341 gcgcacggcg ctgggggccg gccactggcg ctcggtgacg cggggcaggg cggctggcgc
     8401 ttcggtggcg gctgaatctc gcgtcagcac ccgccgcgca ccgttgaagc gcttctgcca
     8461 gtagctgatg tccatgctct cgacgcgcac ctgcgcgccg gcgcgtgggg catggatgaa
     8521 cttgccatcg cccagataga tgccgacatg gctgtaggcg cggcgcaggg tgttgaagaa
     8581 caccagatca ccgggctgca gatcggtctt ggtgatctgg gcggtggagg ctgcctgttc
     8641 agcggccgtg cgcggcagga tcagtcccac ggcctgctga tacatgctgc gcacgaaccc
     8701 gctgcagtca aatccggttt cggccgacgt gccgccacgg cggtagggta cgcccagtgc
     8761 gcccatggcg ttgcggatca gatcggtggc gcggtcggtg gcctggtgga tgctgtcact
     8821 gagcctggtg gtcaggccct tgtgttccag cagacgttcc atatcctgct gcgcgggctc
     8881 ggcatgggcc tggtgcacgg aggcagccag caggatcaag gcgacagcga aggtcttggg
     8941 catcagggcg ttgtgagcgg cgaggtgcgg cttgcgtgag gaaggcaaac gcgaggccgc
     9001 agaagaggtg aaatcgaata ctttcttatc tgttcgtacc catttctggg gagtcgtcgt
     9061 agtgtaggcc atggcactga cctgccaagc cggtgacatt cgctcgccat ccggatttac
     9121 cccaggattg cgtgatatgc cacgccgaaa tgcgcatgtc gaccaggttt gcacgccatg
     9181 gcgggacacc gagccggcat ggcccgcgcc atcgcggcgt cctgcggctc gatcgcgcgc
     9241 aagctggcgc ggctcttgct tcgctgttgg tcatggatga gcaacttcgc atcgtggtcg
     9301 tggcccccga cctggccccc agcaacccgg ccgacgagcg cgccatgcgc caggccgagc
     9361 gctcgcgcgc gctgcgcata ggcctgctgc agcatggctt caacctcgtg gccgtgctgc
     9421 cggccgatgc gctgctggag ggtcatctga cggcgctgca gccggacctg gtcatcgtcg
     9481 acgccgagag cgaggcacgc gacacgctcg agcatgtggt gctggccacg cgcgaggcgc
     9541 cgcggcccat cgtgctgttc accgacgacg aggacacgac ccatgtcaag gacgccatgg
     9601 ctgcgggcgt cacggcctat gtcgtcgccg gcctggcgcc gcagcgcata cgcccgattc
     9661 ttgaggtggc cctggcccgc tttgcccatg agcaggcgct gcgcaccgaa ctggtgcagg
     9721 cacgcaccgc tttgcacgag cgcaagctca tagaccgggc caagggcctg ctgatgcagc
     9781 gccagtccat cgccgagcag caggcctacg accggctgcg ccgcgccgcc atggaccgcg
     9841 gcctgcgcgt ggccgaggtg gcgcagcgca tgctggacgc ggcagatttg ctcggctgag
     9901 ccagggcagg caacactggc acaccacttg ctttaggttt ttcaggccaa cgcaggtctg
     9961 ccccgcgcac tttcaacggc gaaagcggcg caccccagga caaaggcgtc ctcacgcatt
    10021 cggtgctcca ccggacggcg tgagggcgct tttttttgcc ccggattttt cacagtaaag
    10081 gaaccaccat gacagaactg cccaccaccg ccgccgtcac caccactgcc gcggcccccg
    10141 ccaccggcct gccgcgccgc caactgctgc aggccactgc cgtcggcgcc gtcggcatca
    10201 gccccgcgct gcgcgcgctg gtgcatgccc agggctcgga cgcgcccgag aagaaggagg
    10261 tcaagatcgg cttcatcccg ctgaccgact gcgccagcgt ggtcatggcc tcggtgctgg
    10321 gctttgacaa gaagtacggc gtgaccatca ccccgaccaa ggaggcgagc tgggccggcg
    10381 tgcgcgacaa gctggtcaac ggcgagctgg acttcgccca tgtgctctat ggcctggtgt
    10441 acggcgtgca cctgggcacg gccgggccaa agaaggacat ggcggtgctg atgaacctga
    10501 accacaacgg ccaggcgatc acgctgtcca aggccctggc cgacaagggc gcggtcgatg
    10561 gcccgtcgct cgccgcgctg atggccaggg acaagcgcga atacacgttt gccggcacct
    10621 tccccacggg cacgcatgcc atgtggatgc attactggct ggcggccgcg ggcatcaatc
    10681 cgctgtcggg cgccaagctg atcaccgtgc cgccgccgca gatggtggcc aacatgcgcg
    10741 tgggcaacat ggacggcttc tgcgtgggcg agccctggaa ccagcgcgcc atcatggacg
    10801 gcataggcat cacggccaac accacgcagg acatctggcg cgatcacccc gagaaggtgc
    10861 tgggcacgac ggccgacttc gtcaagaaat accccaacac cacgcgcgcc gtgatgatgg
    10921 cggtgctgga agccagccgc tggatagacg ccagcctgtc gaacaagacc aggatggccg
    10981 agaccattgc cgccaagtcc tatgtgaaca ccggcgtgga tgccatcaac cagcgcatcc
    11041 tgggccgcta ccagaacggc ctgggcaaga gctgggacga ccccaaccac atgaagttct
    11101 ttggcgacgg cgcggtcaac ttcccctatc tgagcgacgg catgtggttc ctgacccagc
    11161 acaagcgctg gggcctgctc aagagccacc ccgactacct ggccgtggcc aagcaggtca
    11221 accaggtgga gctgtaccgg tcggtggcca gcgccatgaa gatcagcgtg cccaaggacg
    11281 cgatgcgcag ctccaagctg atcgacggcg tggtgtggga cggcaagaac ccggcccagt
    11341 acgccgacgg tttcaagatc aaggcccaag aggcgtgaac ctttttttcc gagaggagat
    11401 caccatggtc agtgccgtct ttcatgcccc gctggactcc agcgcggaca acacaaaaac
    11461 catagctgcc agcgctttat ccacgggcgc ggcagcccaa aaagatgcaa aacccgccaa
    11521 tccacccgcg gcgcggccag cgcgtgactg gggcgcgctc tcgcgcgccc tgtggctcgc
    11581 ggtgctgccg cccgtgctag gtgtggcgct gctcgtcggc ctgtgggcgc tggtagccag
    11641 cagcacgggc ggcagcatcc ccagcccgca cgacacctgg ctgcaggcgc aggaggtctt
    11701 cagcgacccc ttctaccgca acggccccaa cgaccagggc gtgggctgga acgtgctggc
    11761 cagcctgcag cgcgtggccg tgggctttgg cctggcggcg ctggtgggca tacccgcggg
    11821 ctttgccatc gggcgctttg cctttctcgc gcgcatgttc aacccactca tcagcctgct
    11881 gcggccggtc tcgcccctgg cctggctgcc cataggcctg ttggtgttca agggcgccaa
    11941 ccccgccgcc atctggacca tcttcatctg ctccatctgg cccatggtca tcaacaccgc
    12001 cgtgggcgtg cagcgtgtgc cgcaggacta cctgaacgtg gcgcgcgtgc tgaacctgag
    12061 tgagtggaag gtgctgacca agatcctgtt tccctcggtg ctgccctaca tgctgaccgg
    12121 cgtgcgcctg gccgtgggca cggcctggct ggtgatcgtc gcggccgaga tgctcacggg
    12181 cggcgtgggc ataggcttct gggtctggga cgaatggaac aacctgaacg tcaagaacat
    12241 catcatcgcc atctttgtga tcggcatcgt cgggctgctg ctcgaactcg ccctggtcaa
    12301 gctggccacg gccttcacgt ttgaagaggt caagacctga cccacccctg agccgcgccg
    12361 accggcgcgt caccccctca agggggcgcc accagtggcc cggcaaagcc ggctccacgg
    12421 tggccctggc aaattcaagg agcaacgtca tgaatgcatc tctcactacc aagtacatcg
    12481 aaatccaggg cgtggagcag acctttcgca ccgccaaggg tgtctttcct gccctgcgcg
    12541 acgtgaatct gaccatcgcc aagggcgagt tcgtggccct gatcggccat tcgggctgcg
    12601 gcaagagcac gctgctcaac ctgatcgcgg gactcaccag ccccacgggc ggcgcgctgc
    12661 tgtgcgccaa caaggagatc aagggcccgg gccccgagcg cgccgtggtg ttccagaacc
    12721 attcgctgct gccctggctg acctgcgagg acaacatcta cctggccgtc gagcgcgtct
    12781 tcggcgcccg ggaaaccaag gcccagctca agctgcgcac cgccgcggcc ctggccctcg
    12841 tggggctgac gcacgcgggc cagaagcgcc cgggcgagat ctccggcggc atgaagcagc
    12901 gcgtgggcat cgcccgcgcc ttgagcatgg agccgcaggt gctgctcatg gacgagccgt
    12961 ttggcgcgct cgacgcgctc acgcgcgcca agctgcagga cgagctgctg gacatcgtgg
    13021 cgcgcacgca cagcaccgtg gtcatggtca cgcacgacgt ggacgaggcc gtgctgctgt
    13081 ccgacaagat cgtgatgctg accaatgggc ccgcggccac gataggcgag gtgctgcagg
    13141 tcgacctgcc gcgcccgcgc tcgcgcgtgg agctggccga ggacgcgcgc taccagcagt
    13201 accgcaaggc ggtgatcgac ttcctctaca cgcgccaggg gcatgtggag aaggcggcct
    13261 gaccgcccct gccctcacga cagacagccc ggtgtcgcca ccgggctgtt ttgtttggga
    13321 agagtgtttg caacgcggcc ggcacgggca gggcctcacg gccttgccct gccctatgcc
    13381 atgctggcct gcaccatgcg ctgcagctgc ggcacgcagg agccgcattg cgtgccgcag
    13441 cgcagctcct ccttcaggcg cgccaggcgt gcgccggcgt cgcccgcgca ggccttgagc
    13501 aggccggtga tggcgttctc gcccacgccc tcgcaggcgc agacggtggc gccgcggggc
    13561 gcgagcggct gtggcggctc ggcgccgggc tgcagcaggg cgcggcgcag cggctgggcg
    13621 tcgtggcact cctgcagcag cggtgcgatc caggcctggg cgctgacgtc accggccagc
    13681 agcatggcgt gcagttgttg ccccttgccc gcgggctcca ggcgcgcgcg gcgccattgg
    13741 ccgcgtcgcg cgtcgcggta ggccagcagc cccgcgccct gcaagccgag caggccggcg
    13801 atgcgctcga tcagcgcctc atcgacggcc tggtgcgcgg ccgcgcgcag ctgcacgccg
    13861 ctgccgacct cggcgcggcc gaacaggctg cagctggcaa aggggaactc gtccatcagc
    13921 gcctcgagct gctggcgcac gcgctgcacc tgggccagcg gcagccaggc ctgggcccac
    13981 aggccccagg gcagctcggc cttgagcacc ttgatggccg cgtgcttgag ctcgggctgc
    14041 agcgattgcg ggcagcgcgc gccgctggtc agggcgttga cgccggccag gcgccggccc
    14101 gtgctgctgg caccgcccag ggcctcgtcg ccccagtgca tgggcagaaa cacctggccg
    14161 ggcgcgagtg tgccgctggc ctgcaccggc accacgatgc tgccgcgccg gctcgtgagg
    14221 taggccaggt cgccgtcctt caggcccagg cgcgccatgt cggcggcgtg catgtcggcg
    14281 cgcggctcgc tggcgtgggc ccagaggctt tcgaccaggc cgctgcggct catgccatgc
    14341 cactggtcgc gcaggcgccc ggtgatcagc gcgaaggggt ggcgcgcgtc ggtgggctcg
    14401 gccaccgtgc cgggcagctg cagggcaaag cgggcgcggc cgtcggcgct ggcgaactgc
    14461 cggtcggtgt acaggcgcgc acggccgtgc ttttcgccct cggggcaggg ccattggcgc
    14521 ggcgcggctt ccagctgcgc ccaggtcagg cccgtgatgt ccaggtcgcg cccgcgcgtg
    14581 ctctcgcggt gctcgttcca gatggcctcg gggccgtcgt aggggaacag gctgggca
//
LOCUS       DAOQTZ010000070        14470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4716_length_14470_cov_12.983212, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000070 DAOQTZ010000000
VERSION     DAOQTZ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4716_length_14470_cov_12.983212"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PKN51_09180"
     CDS             <1..701
                     /locus_tag="PKN51_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNT85310.1"
                     /translation="KMITKNTTIPTKFAQTFSTADDNQPAVTIKVFQGEREVASGNKL
                     LGEFNLEGIPPAPRGLPQIEVTFDIDANGILHVSAKDKGTGKENKITIKANSGLSESE
                     IQQMVKDAELNAAEDHKKVELVQARNQGDAMVHSVRKSLGEHGDKLDAGEKDKIEAAI
                     KALEEAVKGDDKADIESKTEALMTASQKLGEKMYADAAAAAQAAGAAPEGAQAGAAAD
                     DNVVDAEVKEVKKD"
     gene            821..1948
                     /gene="dnaJ"
                     /locus_tag="PKN51_09185"
     CDS             821..1948
                     /gene="dnaJ"
                     /locus_tag="PKN51_09185"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNT85311.1"
                     /translation="MAKRDYYEVLGVPKNASEDEIKKAYRKLAMKYHPDRNQGDAKSA
                     EAKFKEAKEAYEMLSDPQKKAAYDQYGHAGVDPNMRGGAEGFGGFAETFGDIFGDIFG
                     GGRGGARGGRQVYRGADLSYAMDITLEEAAAGKEAQIRIPSWDDCDTCHGSGAKPGTS
                     ASTCTTCHGQGVVQMRQGFFSVQQTCPHCRGTGKVIKDPCTSCHGQGKVKNQKTLEVK
                     IPAGIDDGMRIRSAGNGEPGSNGGPPGDLFIEIRLKKHDIFERDGDDLHCVVPIGFAT
                     AALGGEIEVPTLSGKAAIDIPEGTQAGKQFRLRGKGIKGVRSSYPGDLYCHISIETPV
                     KLTEHQRKLLKEFDESLRKGGAKHSPGTESWTDRLKSFFGA"
     gene            complement(2075..2545)
                     /locus_tag="PKN51_09190"
     CDS             complement(2075..2545)
                     /locus_tag="PKN51_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HNT85312.1"
                     /translation="MSAPDFDLQRDAFGRLVLTDAQGHAHVGVAAVRAFPIGAPDEGI
                     SLVDGDGHELAWVPRLGALDEDRRALLVEALEQREFMPRLLRVRGVSSFVTPSTWEVD
                     TDRGPTRFVLKGEEDIRRLGPGLLIVNDAHGVQYLIQDLMAMDRHSRKLLDRFL"
     gene            complement(2542..4854)
                     /locus_tag="PKN51_09195"
     CDS             complement(2542..4854)
                     /locus_tag="PKN51_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85313.1"
                     /translation="MPAGDPAPAIEEAPPLRPTENVLGSLDVDLTRQLRFGRGVILIT
                     DQRLVTRFPDATGWQAWDLHPGLSLAHHDHAGVGTLELHDAQGRLAVWRYTLGHNPAA
                     LRLLGQFERAREALAGGAPSASEDVAPADQAEDEEGVPDQPPSTWTLLRLWRFARPYQ
                     WQLLAGFVLMLAATAATLVPPYLTMPLMDKVLIPFQSGQQIDTGYVALLLTGLLGSAL
                     LAWGLGWAKTYILALVSERIGADLRTITYEHLLKLSLEYFGGRRTGDLMARIGSETDR
                     INIFLSLHLLDFATDVLMIAMTAAILFSIDPTLALVTLVPLPIIAWLIHVVRDRLRTG
                     FEKIDRVWSEVTNVLADTIPGIRVVKAFAQEDREASRFRDANRHNLAVNDRLNRLWSL
                     FSPTVTLLTEIGLLIVWAAGIWLVSQQRVTVGVLTAFLAYIGRFYTRLDSMSRIVSVT
                     QKAASATKRIFEILDHVSSVPEPKNPVPLPRVQGRIDIRSAALRYGNRSVLRGLDLHI
                     EPGQMIGLVGHSGSGKSTLVNLICRFYDVSEGAIEVDGVDLRQVRVADWRRHIGLVLQ
                     EPYLFFGTIAENIAYGKPDATREEIVAAARAAHAHEFILRLPQGYDSLVGERGQGLSG
                     GERQRISIARALLIDPKILILDEATASVDTETEREIQKALDNLVRGRTTIAIAHRLST
                     LRKADRLVVMDKGVKVEEGTHEVLLGRRGAYWRLYEAQARQEKAERERMLVVDDAVLA
                     GEPTAVPAIATPTHRGDVSTAEARRQESQP"
     gene            5081..7204
                     /gene="cphA"
                     /locus_tag="PKN51_09200"
                     /pseudo
     CDS             5081..7204
                     /gene="cphA"
                     /locus_tag="PKN51_09200"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
     gene            7288..9960
                     /gene="cphA"
                     /locus_tag="PKN51_09205"
     CDS             7288..9960
                     /gene="cphA"
                     /locus_tag="PKN51_09205"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNT85314.1"
                     /translation="MEITRIRALRGPNLWSRHTAIEAVVTCDAAERDLHQQPTFEQRL
                     RELFPHVGTLQVNQPAAPSTPLSMAHALELVTLALQAQAGCPVTFSRTTPTEIEGTYQ
                     VAVQYTEEAVGRLAFDKAQLLCRAAAEGGQFDVVAALAELRELDEDERLGPSTGAIVD
                     AAVRRGIPFKRLTRGSLVQLGWGSRQRRIQAAEVDGTSAIAESIAQDKDLTKKLLHAA
                     GVPVPLGRPVGDLQDAWAAAQEIGLPVVVKPQDGNQGKGVTVGILDREHFDIAYQAAA
                     QYGTVMVERFLPGHDFRLLVVGNQLVAAARREPPLVVGDGEHTVRELVAEVNKDPRRG
                     EGHGTSLTRIRIDAIAEARLAAQGLTPDSVPTKGQRVVMRNNANLSTGGTATDVTDSV
                     HPLVAARAIEAAQTVGLDICGVDVVCETVIKPLEEQNGGIVEVNAAPGLRMHLSPSYG
                     KGRDVGQAVIDHMFPDGGNGRIPVIAVTGTNGKTTTVRLAAHLLKTSGLRVGMTNTDG
                     VYVNGRQIDSGDCSGPRSARNVLAHPDVDAAVLETARGGMLREGLAFDQCDVAIVTNL
                     GGGDHLGLNYITTLEDLKVLKRVIVLNVAPTGTAVLNAADPAVVAMAPHCPGQVTYFA
                     LDGHHPVIATHRAQGKRVVFVDRGDIVCAEGDSLRRLPLSRVPLTRKGQIGFQVENCM
                     ASVAAAWALGVSWDAIQKGLASFISDLHGVPGRFNVFDYRGATLIADYGHNPDAIAAL
                     VAGVQNMPASRRSVVISGAGDRRDEDIRGQTRLLGAAFDDVILYQDACQRGRQDGEVL
                     ALLRQGLEGATRTRHVEEIRGEFKAIDRALGRLQPGDLCLILIDQVEEALAHIQQRVA
                     EAAPSPAAPRPSASARKPRRAKPASTVVALGLRL"
     gene            complement(10060..11016)
                     /locus_tag="PKN51_09210"
     CDS             complement(10060..11016)
                     /locus_tag="PKN51_09210"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNT85315.1"
                     /translation="MSRLPPVLQRLKFPVIGSPLFIISNPKLVIAQCTSGVVGSMPAL
                     NARPAAQLDEWLAEITEALAAWDRAHPDNPAAPFAINQIVHKSNDRLEHDMQLCAKYK
                     VPIIITSLGAREDVNHAVHGWGGVVLHDVINNVFAHKAIEKGADGLIPVAAGAGGHAS
                     AKSPFAMVQEIREWFDGPIALSGAIASGGAILAAQAMGADFAYIGSAFIATEEARAVD
                     AYKQMICDCDSDDIVYSNFYTGVLGNYLKGSIRNAGMDPDNLPQSDPSKMNFGGGGGA
                     KAWKDIWGCGQGIGAIRQVVPTAVLIERFKREYQQARERLAL"
     gene            complement(11107..12894)
                     /locus_tag="PKN51_09215"
     CDS             complement(11107..12894)
                     /locus_tag="PKN51_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNT85316.1"
                     /translation="MSYKAPVKDLLFNIEHLARIDQIAAIPGFEDAGLETAQAVLEEC
                     ARFNEGVVAPLNFEADKAPSSHSGTSVTTSAGFKEAYRQYIEGGWQGLQHPAAFGGQG
                     LPKTIGAACGEVLNSANLSFALCPLLTDGAIEALLTAGSEELKATYLEQLVSGHWTGT
                     MNLTEPQAGSDLAAVRTRAEPQPDGTYRIFGTKIFITYGEHDMAENIVHLVLARVQGA
                     PEGVKGISLFVVPKFMVKPDGSLGERNDVYCVSIEHKMGIKASPTAVLQFGDHGGAVG
                     YLVGEENRGLEYMFIMMNAARYAVGVQGIAIAERAYQKAVQYAGERVQSRPVDGSTPG
                     AATIIHHPDVRRMLMTMRALTEGCRAMASTAAAAYDAAHHHPDADVRRQNQVFYEFLV
                     PLVKGYSTEMSLEATNLGVQVHGGMGFIEETGAAQYYRDAKILTIYEGTTAIQANDLV
                     GRKTARDGGQTARAVAAQIEATERQLAGGSSDAQAVARRLATARQAFLDVVDFMAAQA
                     RANPNAAYAGSVPYLMLTGNLVAGWQLGRALIAAEQQKAAGHDAAFMTAKIATARFYA
                     DHILTRVPGVRDAIVEGADSVTALAPDAF"
     gene            complement(13161..14093)
                     /locus_tag="PKN51_09220"
     CDS             complement(13161..14093)
                     /locus_tag="PKN51_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNT85317.1"
                     /translation="MTALVIAEHDNASIKGATLNTVTAAAACGGDVHVLVAGHNAAAA
                     AQAASQIAGVAKVLHADAPALAHGLAENLAAQVLAVAGNYSHILFPATAGGKNAAPRV
                     AARLDVAQISEVTKVDSPDTFERPIYAGNAIATVQSADATKVITVRATGFDAAAASGG
                     SAAVESVAAVADSGKSGFVGQEIAKSDRPELTAAKIVVSGGRALGSAEKFNEVMTPLA
                     DKLGAAIGASRAAVDAGYAPNDLQVGQTGKIVAPQLYIAAGISGAIQHLAGMKDSKVI
                     VAINKDEEAPIFSVADYGLVADLFEAVPALTQAL"
     gene            complement(14223..>14470)
                     /locus_tag="PKN51_09225"
     CDS             complement(14223..>14470)
                     /locus_tag="PKN51_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNT85318.1"
                     /translation="LPAVVTTDLRLNEPRYVTLPNIMKAKKKPLDTVKPEDLGVDVAP
                     RLKTLKVAEPPKRGAGIKVPDVATLVDKLRNEAKVIG"
BASE COUNT         2408 a         4987 c         4883 g         2192 t
ORIGIN      
        1 ccaagatgat caccaagaac accaccatcc cgaccaagtt cgcccagacc ttctccaccg
       61 ccgacgacaa ccagccggcc gtgaccatca aggtgttcca gggtgagcgc gaggtggcca
      121 gcggcaacaa gctgctgggc gaattcaacc tggaaggcat tccgcccgca ccgcgcggcc
      181 tgccgcagat cgaagtaacc ttcgacatcg acgccaacgg catcctgcac gtcagcgcca
      241 aggacaaggg cacgggcaag gaaaacaaga tcaccatcaa ggccaactcg ggcctgtccg
      301 agtccgagat ccagcagatg gtcaaggacg ccgagctgaa tgccgccgaa gaccacaaga
      361 aagtcgagct ggtacaggcg cgcaaccagg gcgacgccat ggtgcacagc gtgcgcaagt
      421 cgctcggtga gcacggcgac aagctcgacg ccggcgagaa ggacaagatc gaggccgcca
      481 tcaaggccct ggaagaagcc gtcaagggcg acgacaaggc cgacatcgaa agcaagaccg
      541 aggccctcat gacggccagc cagaagctgg gcgagaagat gtatgccgac gccgcagccg
      601 ccgcccaggc cgccggcgct gccccggaag gggcgcaagc cggcgcggcc gccgacgaca
      661 acgtggtcga cgccgaggtc aaggaagtca agaaggactg acgcccatga acgcgccgcc
      721 ggccagcgca aaggccggcg cgcgcccgcc gcgcagagga ggtctggcaa cagacaccac
      781 cctgcgcggc gttgttgctt gagagatgga aagaacgcag atggccaagc gcgattacta
      841 cgaagtcctg ggtgttccca agaacgcgtc cgaggacgag atcaagaagg cttatcgcaa
      901 gctggcgatg aagtaccacc cggaccgcaa tcagggtgac gccaagagcg ccgaggccaa
      961 gttcaaggag gccaaggaag cctacgaaat gctgtccgac ccgcagaaga aggccgccta
     1021 cgaccagtac ggccatgcgg gggtggaccc caacatgcgc ggcggagcag aaggcttcgg
     1081 cggctttgcc gagacctttg gcgacatctt cggcgacatc ttcggtggcg gccgcggtgg
     1141 cgcgcgcggt ggccggcagg tgtaccgcgg cgccgatctc agctacgcga tggacatcac
     1201 gctggaagag gctgccgccg gcaaggaagc gcagatccgc atcccgagct gggacgactg
     1261 cgacacctgc cacggctccg gcgccaagcc cggcaccagc gccagcacct gcaccacctg
     1321 ccacggccag ggcgtggtgc agatgcgcca gggttttttc agtgtgcagc agacctgccc
     1381 gcactgccgc ggcaccggca aggtcatcaa ggacccctgc accagttgcc acggccaggg
     1441 caaggtcaag aaccagaaga ccctggaggt gaagatcccc gccggcatcg acgacggcat
     1501 gcgcatccgc tcggccggca acggtgagcc gggcagcaac ggcgggccgc cgggcgatct
     1561 gttcatcgag atccgcctga agaaacacga catcttcgag cgcgacggcg acgacctgca
     1621 ctgcgtggtg ccgatcggtt tcgccaccgc ggcactgggc ggcgagatcg aggtgcccac
     1681 gctcagcggc aaggcggcga tcgacattcc cgaaggcacg caggcgggca agcagtttcg
     1741 cctgcgcggc aagggcatca agggcgtgcg ctcgtcctac ccgggcgacc tgtactgcca
     1801 catcagcatc gaaacgccgg tcaagctgac cgagcaccag cgcaagctgc tgaaggagtt
     1861 cgacgaatcg ctgcgaaaag gcggcgccaa gcactcaccc ggcaccgaaa gctggaccga
     1921 ccgcctcaag agcttcttcg gcgcctgacc ggcgccccgg cgcgaccgcc ccgctcaagg
     1981 acgtcgcaac accgctgccg ccccaacgat ttcaagtcaa aacgacctct ggcccatgcc
     2041 tggcaagcgc gagcagctat atttgttgta gcgttcagag gaagcggtcg agcaacttcc
     2101 ggctgtggcg gtccatggcc atcagatcct ggatcaggta ctgcacgcca tgggcgtcgt
     2161 tgacgatcag caggccgggc ccgagacggc ggatgtcttc ctcacccttg agcacgaagc
     2221 gggtcggacc gcggtcggtg tcgacctccc aggtgctcgg cgtcacgaaa ctgctcacgc
     2281 ccctcacgcg cagcagacgc ggcatgaact cgcgctgctc cagcgcctcg accagcaggg
     2341 cgcgacggtc ctcgtccaat gcgccgagcc gcggcaccca ggccagttca tgaccgtcgc
     2401 catcgaccag gctgatccct tcgtcgggcg caccgatcgg aaacgcccgc acggccgcca
     2461 cgccgacatg cgcatgcccc tgggcgtcgg tcagcaccag gcgaccaaag gcgtcgcgct
     2521 gcagatcgaa gtcgggcgcg ctcatggctg ggactcctgg cgccgggcct cggccgtgct
     2581 gacgtcgccg cggtgcgtcg gcgtggcaat ggcgggcacc gccgtcggct caccggccag
     2641 cacggcatcg tcgaccacca gcatgcgctc gcgctcggcc ttctcctgcc gcgcctgcgc
     2701 ctcgtacagg cgccagtagg cgccgcggcg ccccagcagc acttcgtgcg tgccctcctc
     2761 gaccttcacg cccttgtcca tcaccaccag ccggtcggcc ttgcgcagcg tggacaggcg
     2821 gtgcgcgatg gcaatcgtcg tgcgcccacg caccaggttg tccagcgcct tctggatctc
     2881 gcgttcggtt tcggtgtcga ccgaggccgt ggcctcatcc aggatcagga tcttggggtc
     2941 gatcagcagc gcgcgggcga tgctgatgcg ctggcgctcg ccgcccgaca gcccctggcc
     3001 gcgctcgccc accagcgagt cgtagccctg cggcagccgc aggatgaact cgtgcgcgtg
     3061 ggcggcgcgc gcggcggcca cgatctcctc gcgcgtggcg tcgggcttgc cgtaggcgat
     3121 gttctcggcg atggtgccga aaaacaggta gggctcctgc agcaccaggc cgatgtggcg
     3181 gcgccagtcg gccacccgca cctgccgcag gtccacgcca tcgacttcga tggcgccctc
     3241 gctcacgtca tagaagcggc agatcaggtt caccagcgtg ctcttgccgg agccgctgtg
     3301 gcccaccagg ccgatcatct gacccggttc gatgtgcagg tccaggccgc gcagcaccga
     3361 gcggttgccg tagcgcagcg ccgcactgcg gatgtcgatg cggccctgca cgcgtggcag
     3421 cggcacgggg tttttcggct ccggcacgct ggagacatgg tcgaggattt caaagatgcg
     3481 cttggtggcc gaggccgcct tctgcgtcac cgagacgatg cggctcatcg aatcgagccg
     3541 ggtgtagaag cgcccgatgt aagccagaaa cgccgtcagc acccccaccg taacgcgctg
     3601 ctggctgacc agccagatgc cggcggccca gacgatcagc agcccgattt cggtcagcaa
     3661 cgtgacggtg ggcgaaaaca gcgaccacag gcggttcagg cggtcgttga cggccaggtt
     3721 gtggcggttg gcatcgcgaa agcgactcgc ttcgcggtct tcctgggcga aggccttgac
     3781 cacgcggata cccggaatgg tgtcggccag cacgttggtg acttccgacc agacgcggtc
     3841 gatcttctcg aagccggtgc gcaggcggtc gcgcaccacg tggatcagcc acgcaatgat
     3901 cggcagcggc accagggtca ccagcgccag cgtcgggtcg atggaaaaca ggatcgccgc
     3961 cgtcatggcg atcatcagca cgtcggtggc gaagtccagc aggtgcagcg acagaaaaat
     4021 gttgatgcgg tcggtttccg aaccgatgcg cgccatcagg tctcccgtgc gccggccacc
     4081 gaaatattca agtgaaagtt tgagcaggtg ttcgtaagtg attgtccgca ggtcggcacc
     4141 gatgcgctcg gacaccagcg ccaggatgta ggtcttggcc cagcccaggc cccacgccag
     4201 caaggccgac cccagcaggc cggtcagcag cagcgcgacg tagccggtgt cgatctgctg
     4261 gccgctctgg aacgggatca gcaccttgtc catcagcggc atggtcaggt agggcggcac
     4321 cagggtggcg gcggtggccg ccagcatcag cacgaaaccg gccagcagct gccactggta
     4381 gggcctggca aagcgccaca ggcgcagcag ggtccaggtg gacggcggct ggtccggcac
     4441 accctcttca tcctcggcct ggtcggccgg ggcgacgtct tcgctggccg acggcgcgcc
     4501 gccggccagc gcctcacggg cgcgctcgaa ctgccccagc aggcgcagcg ccgccgggtt
     4561 gtgccccagg gtgtagcgcc agaccgccag ccttccctgc gcgtcgtgca gctccagcgt
     4621 gcccaccccg gcgtggtcgt ggtgcgccag cgacagcccg gggtgcaagt cccaggcctg
     4681 ccagccggtt gcatccggaa accgcgtgac aagccgttga tctgtaatga gaatcacccc
     4741 gcgcccaaag cgcagctggc gcgtcaggtc aacgtccagc gagcccagca cgttttctgt
     4801 gggtcgcagg ggtggcgcct cttcgatggc aggcgcgggg tcacccgccg gcatggccag
     4861 gcggggatcg gacgacgtgg gttccggcaa ggcagccctt cagacagcaa cgaaacacgg
     4921 aaagcgagcg cagggcccca tgaggcgaat cgatcaacac gaacacggct cggcaaacgg
     4981 tggcggattg tatgtccgcg ccccacaagc cgccgccgaa ctggcgccgg cgtcggccgc
     5041 accgcccgat cgccagttcg ccgacatcat catccgccgc atggcgcatc tgcgcggccc
     5101 caacatctgg acctaccgcg cggtgatcga ggccatcgtc gacatcggcg aactggaaga
     5161 cttcccctcc aacaccctgc ccggctttta cgaccgcctg acggcctggc tgcccggtct
     5221 gatcgagcac cgctgcagcg tcggccgtcg cgggggcttt ctgatgcgcc tgcgcgacgg
     5281 cacctggccg ggccacatcc tggagcacgt ggcgctcgaa ctgcagaccc aggccggcat
     5341 gaagaccggc ttcggcaagg cccgcatgac gcacgagcgt ggcgtctaca aggtggtcat
     5401 ccgcacccgt cacgaagccg tcggcaggct ggccatcacc tcggcgcgcg atctggtgat
     5461 ggcggccatc aacgaccgcc cctacgacgt cgccgccacc atcgcccgac tgaccgagat
     5521 ggtcgatcgc cagtgcctgg ggcccagcac ggcctgcatc gtcgacgccg cggtggaacg
     5581 cggcattccc aacatccggc tcaacgacgg caacctggtg cagctcggcc acggcgccgc
     5641 gcaacaccgc atctggaccg ccgaaaccga ccgcaccagc gccatcgccg aaggcattgc
     5701 gagcgacaag gacctgacca agcagctgct ggcggccgcc ggcgtgccgg tgcccgaggg
     5761 ccgcaccgtc agcagtgccg acgacgcctg ggacgccgcc gaggacatcg gcctgcccgt
     5821 ggccgtcaag ccgtcggacg gcaaccatgc gcgcggcgtg tcgctgaacc tcaagacacg
     5881 cgacgaggtc gtgcgcgcct tcgccgccgc cgaacaggaa ggctccgaag tgatcgtcga
     5941 gcgcttcatt cccggccagg agcaccggct gctggtggtc ggtggcaagg tggtggcggc
     6001 cacgcgtggc gaaatcatcc gcgtcagcgg agatggtcgc tccagcatcg gccagctcat
     6061 cgacgcccac gtcaacaccg acccgcggcg cggcgtcgaa gaacatctgc cgctggaaac
     6121 cctgcgccac gacgacccgg tgctgctgct gctgctggag cgccagggcc tgggcatcga
     6181 cagcgtgctg ccgcgcggcg aatcggcgga ggtgcagcgc accggcaaca tgtcgatcga
     6241 cgtcaccggg caggtgcacc ccgacgtggc cgagctggtg gcaatggcca cccgcgtggt
     6301 cgggctggac atcgccggca tcgacctggt ggccgaagac atcggccagc cgctgattcc
     6361 gcagggcggc gcgatcgtcg aagtcaacgc cgggcccggc ctgctgatgc acctcaaacc
     6421 ggccatcggc cgcgcccagc cggtgggcga cgccatcgtc aaccatctgt tccccgccgg
     6481 agccagcggg cgcattccga tcgtcggcat catcggcgac ggcgaaagcg cgctggcggc
     6541 gcgcctgacc gccgccctga tgcagctgca cggcctgcag acggcactgg gctgccgaga
     6601 tggcctgttc ctcggccagc gccggctggc gagcggcggt gcgctgggct acgacatcgg
     6661 tgagcggatg ctcatcaacc gcacagtgca ggccgccgtg ttcgaaacct cgccgcgcca
     6721 catcctgacc gaaggcctgc cctacgaccg ctgccaggtc ggtgtggtca tgtgcatgcc
     6781 cgggccgcag ggcctggagg atctgtacat caccgaggcc gaccagatgc ccaacatcgt
     6841 gcgcacccag gtcgacgtgg tgctgcccga aggggcggcg gtgctgaacg ccgacgacgc
     6901 cgaagtgctg gcgctggccg actacgccga cggcgaagtg ctgctctaca gcctcgaccc
     6961 gcagagcccg gccttgaagg cgcaccgcgc gcgcaagggc cgcgtcgccc tggcccgcgg
     7021 cgatcgcatc gtgctggcgc aaggcccgga ggagtttgcg ctggtcaacc tgtcggcgcc
     7081 cgagttttcc gccatcgccc aggaaccgcc ggccgacgtg cgcccgcatg tgctggcggc
     7141 cgtcgccacg gcctggtcgc tgggcgtgcc ggtggagctg atccgtgccg gtttgttgca
     7201 cttttgtgaa gcggcaccgg ccggcgctgt ccactgagcc accggctcag ccgttgcgta
     7261 gatcagccca ttccttccag gaccatcatg gaaatcaccc gcattcgcgc gctgcgcggc
     7321 cccaacctgt ggagccgcca cacggccatc gaggccgtgg tcacctgcga cgcagccgag
     7381 cgcgacctgc accaacagcc caccttcgaa caacgcctgc gtgagctgtt tccccatgta
     7441 ggcacgcttc aggtcaacca gcccgcggcg cccagcacgc ccctgtccat ggcccacgca
     7501 ctggagctgg tgaccctggc cctgcaggct caagcgggtt gcccggtcac gttcagccgc
     7561 accacgccga ccgaaatcga aggcacgtac caggttgccg tccagtacac cgaagaagcc
     7621 gtgggccgcc tggccttcga caaggcccag ctgctgtgcc gcgccgcagc tgaaggcggc
     7681 cagttcgatg tcgtcgccgc cctggccgaa ctgcgcgaac tcgacgaaga cgagcgcctg
     7741 ggcccttcga ccggcgccat cgtcgacgcc gccgtgcggc gtggcatccc gttcaagcgg
     7801 ctgacgcgcg gcagtctggt gcagctgggc tggggcagtc gccagcggcg catccaggcg
     7861 gccgaagtcg acggcaccag cgcgattgcc gaatcgattg cccaggacaa ggacctgacc
     7921 aagaagctgc tgcacgccgc cggcgtgccc gtgcccctgg gtcgcccggt cggcgatctg
     7981 caggacgcct gggcagccgc gcaggagatc ggcctgccgg tggtggtcaa accccaggat
     8041 ggcaaccagg gcaagggagt gaccgtcggc atccttgatc gcgagcactt cgacatcgcc
     8101 taccaggccg cggcccagta cggcacggtg atggtcgagc gctttctgcc cggccacgat
     8161 ttccggctgc tggtggtagg caaccagttg gtggcggcag cgcgccgcga gccgccgctg
     8221 gtggtcggtg acggtgaaca cacggtgcgc gaactggtgg ccgaagtcaa caaggatccg
     8281 cgccgtggcg aaggtcacgg cacctcgctg accaggatcc gcatcgacgc catcgccgag
     8341 gctcgcctgg cggcccaggg cctgacgccc gacagcgtgc ccaccaaagg tcagcgcgtg
     8401 gtgatgcgca acaacgccaa cctgtccacc ggcggcaccg ccaccgacgt gaccgacagc
     8461 gtccaccccc tggtggcggc gcgggccatc gaggccgcac agaccgtggg cctggacatc
     8521 tgcggcgtcg acgtggtgtg cgagacggtc atcaagccac tggaagagca gaacggcggc
     8581 atcgtcgaag tcaacgccgc gcccggcctg cgcatgcacc tgagcccgtc ctacggcaag
     8641 ggccgcgacg tcggccaggc ggtgatcgac cacatgttcc ctgacggtgg caatggccgc
     8701 atcccggtga tcgccgtcac cggcaccaac ggcaagacca ccaccgtgcg cctggccgcg
     8761 cacctgctca agaccagcgg tctgcgggtg ggcatgacca acaccgacgg cgtctacgtc
     8821 aatggccggc agatcgactc cggcgactgc tccggaccgc gcagcgcgcg caacgtgctg
     8881 gcccaccccg atgtcgatgc cgccgtgctg gaaaccgcgc gcggcggcat gctgcgcgaa
     8941 ggcctggcct tcgatcagtg cgacgtcgcc atcgtcacca acctgggcgg cggcgatcac
     9001 ctgggcctga actacatcac cacgctggaa gacctgaagg tgctcaagcg cgtgatcgtg
     9061 ctcaacgtcg cgcccaccgg caccgcggtg ctcaacgccg ccgacccggc ggtggtggcg
     9121 atggcgccgc actgccccgg tcaggtgacc tactttgccc tggacggcca ccacccggtc
     9181 atcgccacgc accgggccca gggcaagcgg gtggtgttcg tcgaccgcgg cgacatcgtc
     9241 tgcgccgaag gcgacagcct gcgccgcctg cccttgtcgc gtgtgccgct gacccgcaag
     9301 gggcagatcg gcttccaggt cgaaaactgc atggcctcgg tggcggcggc ctgggcgctg
     9361 ggtgtgtcct gggacgccat ccagaaaggc ctggcgagct tcatcagcga cctgcacggt
     9421 gtgcccggcc gcttcaacgt gttcgactac cgcggcgcca ccctgatcgc cgactacggc
     9481 cacaacccgg acgccatcgc tgccctggtg gccggcgtgc agaacatgcc ggccagccgc
     9541 cgctcggtgg tgatcagcgg cgcaggcgat cgccgcgacg aagacatccg cggtcagacc
     9601 cgcctgctgg gggccgcctt tgacgacgtc atcctctacc aggacgcctg ccagcgtggc
     9661 cggcaggacg gcgaggtgct ggcgctgctg cgccagggtc tggaaggcgc cacacgcacc
     9721 cggcacgtgg aggaaatccg tggcgaattc aaggccatcg accgcgcgct gggccgactc
     9781 cagcctggcg atctgtgcct gatcctgatc gaccaggtgg aagaggcgct ggcccatatc
     9841 cagcagcgcg tggctgaagc agccccctcc cctgcggcgc cgcgcccgag cgccagcgcg
     9901 cgcaagccgc ggcgcgccaa gccggccagc accgtggtgg cgctgggcct gcgtctctga
     9961 ccctgtcctg cagcccggcg cccccagggg ccttcctcgt cacaacaaag cccggcatga
    10021 ccgggctttc tgctgtggct gtgagggtgc gccgttcgat cagagcgcca gccgctcgcg
    10081 cgcctgctgg tactcacgct tgaagcgctc gatcagcact gcggtcggca ccacctggcg
    10141 gatggcgcca atgccctggc cgcaacccca gatgtccttc caggccttgg cgccgccgcc
    10201 accaccgaag ttcatcttgc tggggtcgct ctgcggcagg ttgtccgggt ccatgccggc
    10261 gttgcggatg ctgcccttga ggtagttgcc cagcaccccg gtgtagaagt tgctgtagac
    10321 gatgtcgtcg gaatcgcagt cgcagatcat ctgcttgtag gcatcgacgg cacgcgcctc
    10381 ctcggtggcg atgaaggccg agccgatgta ggcaaagtcg gcgcccatgg cctgcgccgc
    10441 caggatggca ccgccggaag cgatggcgcc cgacagggcg atcgggccgt cgaaccattc
    10501 gcggatttcc tgcaccatgg cgaacgggct cttggcgctg gcgtggccgc cggcgccggc
    10561 agccaccggg atcaggccat cggcgccctt ttcgatcgcc ttgtgggcaa acacgttgtt
    10621 gatgacgtcg tgcagcacca cgccgcccca gccatgcacg gcgtggttga cgtcctcgcg
    10681 tgcgcccagg ctggtgatga tgatcggcac cttgtacttg gcgcacaact gcatgtcgtg
    10741 ctccagccgg tcgttgctct tgtgcacgat ctggttgatg gcaaagggcg cggccgggtt
    10801 gtccgggtgg gcgcgatccc acgcggccag cgcctcggtg atctcggcca gccattcgtc
    10861 cagctgcgcc gccgggcgcg cattgagcgc cggcatcgac cccaccacgc ccgaagtgca
    10921 ttgcgcgatc accagcttgg ggttgctgat gatgaacagc ggcgatccga tgacggggaa
    10981 cttcaggcgt tgcagcaccg gtggcagacg ggacatggcg atcctctcag gaaaccagat
    11041 tgaatataaa aaaggcctcc agcgcccgtt ctacgagcgc cagcagctat gaattcagta
    11101 gcaaattcag aacgcgtccg gcgccagcgc ggtcacgctg tcggcgcctt cgacgatggc
    11161 gtcgcgcacc cccggcacgc gcgtgaggat gtggtcggcg tagaagcgcg cggtggcgat
    11221 cttggccgtc atgaaggcgg cgtcgtggcc ggcggccttc tgctgctcgg ccgcgatcag
    11281 cgcgcggccc agttgccagc cggccaccag gttgccggtc agcatcaggt agggcacgct
    11341 gccggcgtag gcggcgttcg ggttggcgcg cgcctgggcg gccatgaagt cgaccacgtc
    11401 cagaaacgcc tgccgggccg tcgccagccg gcgcgccacg gcctgggcat cgctgctgcc
    11461 gccggccagt tgccgctcgg tggcttcgat ctgcgccgcg accgcccggg cggtctggcc
    11521 gccatcgcgc gccgtcttgc ggcccaccag gtcgttggcc tggatggcgg tcgtgccttc
    11581 gtagatggtc aggatcttgg catcgcggta gtactgcgcg gcgccggttt cctcgatgaa
    11641 gcccatcccg ccgtgcactt gcacgcccag gttggtggcc tccaggctca tctcggtgct
    11701 gtagcccttg accagaggca ccagaaattc atagaagacc tggttctgcc ggcgcacgtc
    11761 ggcatccggg tggtggtggg ccgcatcgta ggccgctgcc gccgtgctgg ccatggcgcg
    11821 acaaccctcg gtcagggcgc gcatggtcat cagcatgcgg cgcacgtcgg ggtggtggat
    11881 gatggtggcg gcgcccggcg tgctgccatc gaccgggcgg ctctgcaccc gttcgccggc
    11941 gtactgcacc gccttctggt aggcccgctc ggcaatcgcg atgccctgca caccgaccgc
    12001 atagcgggcg gcgttcatca tgatgaacat gtattcgagg ccgcggtttt cttcgccgac
    12061 caggtagccg acggcgccgc cgtggtcgcc gaactgcagc accgccgtcg ggctggcctt
    12121 gatgcccatc ttgtgctcga tgctgacgca gtacacgtcg ttgcgctcgc ccaggctgcc
    12181 atcgggcttg accatgaact tgggcaccac gaacaggctg atgcccttga cgccctcggg
    12241 cgcgccctgc acgcgcgcca gcaccaggtg cacgatgttc tcggccatgt cgtgctcgcc
    12301 gtaggtgatg aagatcttgg tgccgaagat gcggtaggtg ccgtcgggct gcggctcggc
    12361 gcgcgtgcgc accgccgcca ggtcactgcc ggcctggggc tcggtcaggt tcatggtgcc
    12421 ggtccagtgg ccggacacca actgctccag gtaggtcgcc ttcagctcct cgctgcccgc
    12481 cgtgagcagc gcctcgatgg caccgtcggt cagcagtggg cacagggcga agctcaggtt
    12541 ggccgaattg aggacttcgc cacaggctgc gccaatggtc ttgggcaggc cttgcccgcc
    12601 gaaggccgcc gggtgctgca ggccttgcca gccgccttcg atgtattggc ggtaggcctc
    12661 cttgaagccg gcgctggtgg tcaccgacgt gcccgagtgg ctggacggcg ccttgtcggc
    12721 ctcgaagttg agcggtgcca ccaccccttc attgaagcgc gcgcattctt ccagcaccgc
    12781 ctgggcggtc tccaggccgg catcctcgaa gccggggatg gcggcgatct ggtcgatgcg
    12841 ggccagatgc tcgatgttga acagcaggtc tttgacgggg gccttgtagc tcatggcagg
    12901 gatcctgaaa catgcaaaac aaaagggcat cgcatgcgac gcccttgaac cgagacatcc
    12961 cagtccaccg aaacactgaa atcggaacgg tgctgtccac cagatacggg cacgctgcgg
    13021 cgttgcccat gctcgccaca gcaccggctg ttgctgcgcc tggcgccttg catcgccccc
    13081 cgtctggggt gtccctcgcc gcccatttca atgtttcagt ggaccagcgc gcaccttcgt
    13141 tctggtgccg cgccggacaa tcacagggcc tgggtcagcg cgggcacggc ctcgaacagg
    13201 tcggccacca ggccgtagtc ggccacgctg aagatcggcg cctcttcgtc cttgttgatg
    13261 gcgacgatga ccttgctgtc cttcatgccc gccagatgct ggatggcgcc cgaaatgccg
    13321 gcggcgatgt agagctgcgg cgccacgatc ttgccggtct gccccacctg caggtcgttg
    13381 ggcgcatagc cggcatccac cgccgcgcgg ctggcgccga tggcggcgcc cagcttgtcg
    13441 gccagcgggg tcatgacctc gttgaacttc tcggcgctgc ccagggcgcg accgccggag
    13501 acgacgatct tggcggcggt cagttcgggg cggtcgctct tggcgatttc ctggccaacg
    13561 aagccgctct tgccgctgtc ggccacggca gccacgctct cgaccgcggc gctgccgccg
    13621 ctggccgcgg cggcgtcaaa gccggtggca cgcacggtga tcaccttggt ggcgtcggcc
    13681 gactgcacgg tggcgatggc gttgccggcg tagatcgggc gctcgaaggt gtcgggactg
    13741 tcgaccttgg tgacttcgct gatctgcgcc acgtccagcc tggcggccac acgcggggcg
    13801 gcgttcttgc cgccggcggt ggcgggaaac aggatgtggc tgtagttgcc ggccaccgcc
    13861 agcacctggg ccgccaggtt ttcggccagg ccgtgggcca gggctggggc gtcggcgtgc
    13921 agcaccttgg cgacgccggc gatctggctg gcggcctggg cggcggcagc ggcgttgtgg
    13981 ccggccacca gcacgtgcac gtcgccaccg caggcggccg cggcggtgac ggtgttgagg
    14041 gtggcgccct tgatggaggc gttgtcgtgt tcggcaatga cgagtgcggt catgagaaat
    14101 ctccgggaat ggggtgcgat gacaaatgac ggatgacttc gccgcgcttc aatgcccaag
    14161 ggttcaaggg tcacgccgcg ccagcggcgt catcgaagcg gcgaagccgc gcggtcattc
    14221 ggtcatccga tgactttggc ttcgttgcgc agtttgtcca ccagggtggc gacgtcgggc
    14281 accttgatgc cggcgccgcg cttgggcggc tcggccactt tcagcgtttt caggcgcggc
    14341 gccacatcca cgcccaggtc ctcgggcttg acggtgtcga gcggcttttt cttggccttc
    14401 atgatgttcg gcagcgtgac gtagcgcggc tcgttcaggc gcaggtcggt ggtgaccacc
    14461 gccggcagcg
//
LOCUS       DAOQTZ010000071        14373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4771_length_14373_cov_14.722447, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000071 DAOQTZ010000000
VERSION     DAOQTZ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14373
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_14373_cov_14.722447"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /gene="hisG"
                     /locus_tag="PKN51_09230"
     CDS             <1..217
                     /gene="hisG"
                     /locus_tag="PKN51_09230"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906521.1"
                     /note="short form of enzyme; requires HisZ for function;
                     catalyzes the formation of N'-5'-phosphoribosyl-ATP from
                     phosphoribosyl pyrophosphate; crucial role in histidine
                     biosynthesis; forms heteromultimer of HisG and HisZ;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNT85319.1"
                     /translation="SMELAPLTGMADAIVDLVSTGKTLKANRLVEVEHIMDISSRLVV
                     NQAALKLKTAPIRRIIDAFAQAIGPAI"
     gene            219..1550
                     /gene="hisD"
                     /locus_tag="PKN51_09235"
     CDS             219..1550
                     /gene="hisD"
                     /locus_tag="PKN51_09235"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNT85320.1"
                     /translation="MKFIAAPARLSTLDADFEQQFQQRLHWSAEQDQAIEQRVRDILA
                     DVRARGDAALLDCTARFDGVQAASVAALELRAAELKAAFDGLPAEQREALTQAAARIR
                     RYHEWQKQQGGETATYRDEDGTLLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAIPAHV
                     AGVGEIVMVVPTPRGERNPLVLAAAHVAGVTRAFTLGGAQAVAALAYGTATVPRVDKI
                     TGPGNAYVASAKKHVFGQVGIDMIAGPSEILVLADGSTPADWVAMDLFSQAEHDELAQ
                     SILLSPDAAYIDAVQAAIERLLPTMPRAEIIARSLTGRGALIHTRSMEEACDIANRIA
                     PEHLEVSSREPQRWEPRLRHAGAIFLGAWTSESLGDYCAGPNHVLPTSGTARFSSPLS
                     VYDFQKRSSLIEVSAEGARRLGRIASVLARGEGLQAHARAAELRDPAAATR"
     gene            1672..2940
                     /gene="gltS"
                     /locus_tag="PKN51_09240"
     CDS             1672..2940
                     /gene="gltS"
                     /locus_tag="PKN51_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNT85321.1"
                     /translation="MEITLNGYHTLILAALVMLVGHWLVFRIRLLSAFNIPEPVAGGL
                     LAAAIVYALNVALGVRFKFSTELQTSFMLMFFSSIGLSADFTRLKEGGKPLVVLLVIV
                     SCFIVVQNLIGVGLAAALGLKPLLGLVVGSITLTGGHGTAGAWGPVLEQNYGIAGATT
                     LGIATATFGLVFGGLIGGPVARYLIRQQHRQPMPEAGVRDSIDTISGKPRHGETDSSV
                     RDRRLTFDMLDEPRLITAYSAIETLAMFAACLTAADLVSGWTKGSAIELPTFIWALAS
                     GVVIRNVLTHAFRFEMFDRAIDVFGNASLSLFLAMALLSLRLWELADLAGPVAIILAV
                     QVLVMMAWASFVTYRFMGADYDAAVVAAGHCGFGLGATPTAIANMQAITDRFGPSYKA
                     YLVVPLVGAFFVDLVNATVLQIFTQLPFLR"
     gene            complement(2979..3551)
                     /locus_tag="PKN51_09245"
     CDS             complement(2979..3551)
                     /locus_tag="PKN51_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNT85322.1"
                     /translation="MRARTLALLGVGAYALYRLSQQTGLPPGVHPVTPFRLERWLGRW
                     YEIARIDPPQDRGLTDGVVHHSLLPDGTLQIARRGFDTRLGRWRTTTARARPAHPSRE
                     AHLHLSLVWPVRTSRAVFDIDDADQHALVCGPTHDQLWLLARSPTMRAATRARLLDRA
                     RAAGFDVGRLTWVDHRRSVAVVGRGSSSTH"
     gene            3659..3877
                     /locus_tag="PKN51_09250"
     CDS             3659..3877
                     /locus_tag="PKN51_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNT85323.1"
                     /translation="MKNVTITVDEAVLEWARVEAARRGSSVSRMVGEMLAEKMRQEDA
                     YAQAMRSALRFESWGASDGPYLRLSEVE"
     gene            3874..4332
                     /locus_tag="PKN51_09255"
     CDS             3874..4332
                     /locus_tag="PKN51_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNT85324.1"
                     /translation="MMPPLVFVDTPVFLYAADERDGRKQALAREWVRWCWSRRSGRIS
                     TQVLNELYHNAITRFRARMPVQQARAQVRNLRQWQPPHLDTFTIDGAWALQDRHALGY
                     WDALIVASAQQQGCQYLLCEDLPHQQRFESVQVINPFRVRPDELPLADEH"
     gene            4322..5425
                     /gene="hisC"
                     /locus_tag="PKN51_09260"
     CDS             4322..5425
                     /gene="hisC"
                     /locus_tag="PKN51_09260"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNT85325.1"
                     /translation="MSTDTSTPSPLARIRQDVQSMHAYAVQDSAGLLKLDAMENPHRL
                     PDALQAELGRRLGRVAINRYPGARVAELGAALHAHAAPPAGWSLMLGNGSDELISLLA
                     MACDVPGASILAPLPGFVMYAMSARLQGLAFHGVPLTPDFALDEAAMLAAIERHRPAI
                     LYLAYPNNPTGTLWDAGSIARLIAAQGRQGGLVVMDEAYQPFAAHSWIEVVRAAPEPH
                     RHVLIMRTLSKFGLAGVRIGYLMGEATLLAEIDKVRPPYNVSVLNAECALFALEHADV
                     FAAQAADIRAQRAQLLQALAALPGVTPFPSQANMVLARVPDAARVFAGMRARGVLIKN
                     VSTMHALLANCVRLTVGTADENAQMLAALQASL"
     gene            5422..6021
                     /gene="hisB"
                     /locus_tag="PKN51_09265"
     CDS             5422..6021
                     /gene="hisB"
                     /locus_tag="PKN51_09265"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNT85326.1"
                     /translation="MTTTARSADVARDTAETRIRVRLNLDGSGQATLATGIGFFDHML
                     DQIARHGLIDLDVQCQGDLHIDGHHTVEDVGIAIGQAVRQAVGDKRGITRYGHSYVPL
                     DEALSRVVIDFSGRPGLVLDARFASAMIGGFDTQLVHEFFQGFVNHAQVTLHIDNLKG
                     VNAHHQVETIFKAFGRALRMALTPDARAAGQVPSTKGTL"
     gene            6065..6736
                     /gene="hisH"
                     /locus_tag="PKN51_09270"
     CDS             6065..6736
                     /gene="hisH"
                     /locus_tag="PKN51_09270"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNT85327.1"
                     /translation="MLSIANQTVAVVDYGMGNLRSVAQAVMHAAQDTRVEVVVTDDPA
                     RVRAAHRVVLPGQGAMGDCMHQLHACGLLESVLEAADGKPLFGVCIGMQMLLTHSAEG
                     HTAAGTPGLGLIPGEVLRFDLAGRQAPDGSRYKVPHMGWNRVFQSRPHPLWQGVADGA
                     WFYFVHSYFARPHTPTHAVGETEYGARFASAVARDNIFATQFHPEKSADQGLTLYRNF
                     LNWRP"
     gene            6832..7572
                     /gene="hisA"
                     /locus_tag="PKN51_09275"
     CDS             6832..7572
                     /gene="hisA"
                     /locus_tag="PKN51_09275"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNT85328.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGDMDQATVFSEAPAEMAAHWLGQGA
                     RRLHLVDLNGAFAGKPQNLAAIKAILRQVDDEIPVQLGGGIRDLDTIERYIDAGIRYV
                     IIGTAAVKNPGFLKDACTAFGGHIIVGLDAKDGKVATDGWSKLTGHEVIDLARKFQDW
                     GVESIIYTDIGRDGMLSGINIEATVKLAQALTIPVIASGGLSTMADIEQLCAVESEGI
                     EGVIAGRAIYSGDLDFAAAQARADALNG"
     gene            7582..8361
                     /gene="hisF"
                     /locus_tag="PKN51_09280"
     CDS             7582..8361
                     /gene="hisF"
                     /locus_tag="PKN51_09280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNT85329.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGA
                     DELTFLDITATSDGRDLILPIIEAVASQVFIPLTVGGGVRTVDDVRRLLNAGADKVSF
                     NSAAIANPQVIRDASGKYGAQCIVVAIDARRRSGDELAARGPGWDVFSHGGRRNTGLD
                     AIAWARQMADWGAGEILLTSMDRDGTRSGFDLELTRAVSDAVPVPVIASGGVGSLEHL
                     ADGVQLGGADAVLAASIFHYGEFTVAQAKQHLAARGIPVRL"
     gene            8545..9891
                     /gene="gdhA"
                     /locus_tag="PKN51_09285"
     CDS             8545..9891
                     /gene="gdhA"
                     /locus_tag="PKN51_09285"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNT85330.1"
                     /translation="MSTAASLNDFLRRIEQRDPQQPEFLQAVREVMMSLWPFLEKHPR
                     YREHGLLERLCEPDRVIQFRVAWVDDKGQTQVNRAFRVQHNMAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMSELYRHLGA
                     DTDVPAGDIGVGAREVGFMAGMMKKLSNNAGSVFTGKGIAYGGSLMRPEATGYGTVYF
                     AQEMLRRKQMGFEDRRVSISGSGNVAQYAADKAMELGAKVVTLSDSDGTLVCEGGLTH
                     GMLQDLMEFKNVQRGRLKDFCKQHKLKFEAGKRPWHVPVDIALPCATQNELDAADAKH
                     LIANGVVCVAEGANMPSTLDAVEHFLAAGTLYAPGKASNAGGVATSGLEMSQNAMRLS
                     WTHSEVDLRLHEIMKDIHGNCVRHGERKDGTVNYVEGANVAGFVKIADAMLAQGVC"
     gene            10163..10555
                     /gene="hisI"
                     /locus_tag="PKN51_09290"
     CDS             10163..10555
                     /gene="hisI"
                     /locus_tag="PKN51_09290"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNT85331.1"
                     /translation="MSNWLDQVAWDAQGLVPAIAQEADTGDVLMFAWMNREALARTAE
                     LGRAVYFSRSRGRLWFKGEESGHVQQVHEIRIDCDNDVVLLKVTQQGHRPGIACHTGR
                     HSCFFSVLKDGAWQAVEPVLKDPESIYK"
     gene            10562..10951
                     /locus_tag="PKN51_09295"
     CDS             10562..10951
                     /locus_tag="PKN51_09295"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNT85332.1"
                     /translation="MSHAELLAHLTAVIESRKPAAGGDPDTSYVARLLAKGPDAFLKK
                     IGEEATEVVMAAKDADHGGPRHKVVSEMADLWFHGMVALAHYGFSAADVVAELARREG
                     LSGLEEKALRKARQRDIDDASREGAGT"
     gene            10948..11343
                     /locus_tag="PKN51_09300"
     CDS             10948..11343
                     /locus_tag="PKN51_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85333.1"
                     /translation="MSRNDIIDVEPVGSARDDSLKTWGWVSYLLHLIVAVAAVVPGAQ
                     VSIALLIIALVLDLVKKDDAAGTWQASHFSWRIRSVLWAGVLYLVTFPLFLLFYLPGA
                     IAWVVISIWFLYRIVLGMVRMNAGRPVEA"
     gene            11554..11919
                     /locus_tag="PKN51_09305"
     CDS             11554..11919
                     /locus_tag="PKN51_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNT85334.1"
                     /translation="MSAHADPHCIFCKIVAGQIPSRKVYEDDELFAFHDIHPWAPVHF
                     LMVPKLHVASMAHVGPEHEALLGRIMALAPKLALEQGCQPYPDGGFRIVVNTGAQGGQ
                     EVHHLHVHVMGGPRPWARG"
     gene            12074..12304
                     /gene="tatA"
                     /locus_tag="PKN51_09310"
     CDS             12074..12304
                     /gene="tatA"
                     /locus_tag="PKN51_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNT85335.1"
                     /translation="MGTFSIWHWLIVLLIVVMIFGTKKLKNIGSDLGGAVKGFKDGMK
                     DGAAEADTPPAGQVTNAAAAKDTIDVQAKEKS"
     gene            12379..12858
                     /gene="tatB"
                     /locus_tag="PKN51_09315"
     CDS             12379..12858
                     /gene="tatB"
                     /locus_tag="PKN51_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNT85336.1"
                     /translation="MLDLGISKMALIGAVALIVIGPEKLPRVARTVGTLLGKAQRYVA
                     DVKSEVNRAMDLDELKKMKESVESAGREVEQSIHATQSAFEKDWNEATAGLGGTGTGE
                     DAPIPSWEPAPPQYRRPDKNWRLKRGATPQWYKQRHGVRRQVQSGAARVARYRPKSP"
     gene            12975..13769
                     /gene="tatC"
                     /locus_tag="PKN51_09320"
     CDS             12975..13769
                     /gene="tatC"
                     /locus_tag="PKN51_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNT85337.1"
                     /translation="MSNPTPDPEDELAGTEQPFVVHLMELRDRLIKSMVAVAVVAGAL
                     ALYPGPGALYDLLAAPLVAHLPQGATMIATSVISPFMVPLKILLMAAFLVALPVVLYQ
                     VWAFVAPGLYSHEKRMVLPLVISSTVLFFAGVAFCYFLVFGKVFTFIQSFAPKSITAA
                     PDIEAYLSFVLTMFLAFGLAFEVPIAVVVLARLGVVSIEQLKSFRGYFIVLAFIIAAI
                     VTPPDVVSQLALAIPMCLLYEVGIWAARLFIKHTTAPEDAAPAAGP"
     gene            complement(13847..>14373)
                     /locus_tag="PKN51_09325"
     CDS             complement(13847..>14373)
                     /locus_tag="PKN51_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNT85338.1"
                     /translation="TFENFIQTDAAINPGNSGGALTDIGGHLMGINTAIYSRSGGNMG
                     IGFAIPVSTARQVMESIVRNGQVVRGWIGVEPGELTPELAQTFGVKAEQGVIITGVLG
                     NGPAASAGIRPGDVITQVGGQPTRTVAELLTRIAALVPGEAVPFTLERRGEAVQASVT
                     PAQRPRPRQPALPR"
BASE COUNT         2296 a         4951 c         4893 g         2233 t
ORIGIN      
        1 cagcatggaa ctggcgccgc tgaccggcat ggccgacgcc atcgtcgatc tggtcagcac
       61 cggcaagacg ctcaaggcca accggctggt cgaggtcgag cacatcatgg acatcagctc
      121 gcgcctggtg gtcaaccagg ccgcgctcaa gctcaagacg gcgccgatcc ggcgcatcat
      181 cgacgctttt gcccaggcca tcggccccgc catttgatat gaaattcata gctgctcccg
      241 cacgtctgtc cacgctggat gccgatttcg agcaacaatt tcagcagcgt ctgcactgga
      301 gcgccgagca ggaccaggcc atcgagcagc gcgtgcgcga catcctggcc gacgtgcgcg
      361 cgcgcggcga cgcggcgctg ctggactgca ccgcgcgctt cgatggcgtg caggcggcct
      421 cggtggcggc gctggaattg cgcgccgccg agctgaaagc cgccttcgac ggcctgccgg
      481 ccgagcagcg cgaggcgctg acgcaggctg ccgcgcgcat ccgccgttac cacgaatggc
      541 agaagcagca gggcggcgaa accgccacct accgcgacga agacggcacc ctgctgggtc
      601 agaaggtgac accgctggac cgcgtgggca tctacgtgcc cggcggcaag gcggcctacc
      661 ccagcagcgt gctgatgaac gccatcccgg cccatgtggc cggcgtgggc gaaatcgtca
      721 tggtggtgcc gaccccgcgt ggcgagagga acccgctggt gctggccgcc gcgcacgtgg
      781 ccggcgtcac gcgtgccttc acgctgggcg gcgcgcaggc cgtggcggcg ctggcctatg
      841 gcacggccac cgtgcctcgg gtggacaaga tcaccggccc cggcaacgcc tatgtggcca
      901 gcgccaagaa gcacgtgttc ggccaggtcg gcatcgacat gatcgccggc ccgtcagaga
      961 ttctggtgct ggccgacggc agcacgccgg ccgactgggt ggcgatggac ctgttcagcc
     1021 aggccgaaca cgacgagctg gcacaaagca tcctgctcag ccccgacgcc gcctatatcg
     1081 acgccgtgca ggcggccatc gagcgcctgc tgcccaccat gccgcgcgcc gagatcatcg
     1141 ccaggagcct gaccgggcgc ggtgcgctga tccacacccg cagcatggaa gaggcctgcg
     1201 acatcgccaa ccgcatcgcg cccgagcacc tggaagtcag cagccgcgag ccgcagcgct
     1261 gggagccgcg gctgcgccac gccggcgcca tctttctggg ggcctggacc agcgagagtc
     1321 tgggcgacta ctgcgccggc cccaaccacg tgctgcccac cagcggcacg gcgcgctttt
     1381 cttcgccgct gtcggtgtac gacttccaga agcgcagcag cctgatcgag gtcagcgccg
     1441 aaggcgcgcg ccggctgggc cgcatcgcca gcgtgctggc gcgcggcgag ggcctgcagg
     1501 cgcacgcgcg cgcggccgag ttgcgcgacc cggcggcagc gacacgctga caggccccac
     1561 cggccatcga ccgccgcgcg cggtggtggc atcatgcgtt gcatgcagcg gcgtgccgcc
     1621 caggcaggtg ccgcggcggc gggcggcctg tgggccggag aggagcgcac ggtggaaatc
     1681 acgctgaacg gctaccacac cctgatcctg gcggcactgg tcatgctggt cgggcactgg
     1741 ctggtgtttc gcatccggtt gctgtcggcc ttcaacatcc cggagcctgt ggctggaggg
     1801 ctgctggcgg cggcgatcgt ctatgccctg aacgtcgcgc tgggcgtccg gttcaagttc
     1861 agcaccgaac tgcagaccag cttcatgctg atgttcttct cgtccatcgg gctgagcgcc
     1921 gatttcacgc gactgaagga gggcggcaag cccctggtgg tgctgctggt catcgtctcc
     1981 tgctttatcg tggtgcagaa cctgatcggc gtcggtctgg ctgcggcgct gggactgaaa
     2041 ccgctgctcg gcctggtggt cggctccatc acgctcaccg gcggccacgg caccgcgggg
     2101 gcctggggtc cggtgctgga gcagaactac ggcatcgccg gcgccaccac gctgggcatc
     2161 gccacggcca ccttcggcct ggtgttcggc gggctgatcg gcggtccggt ggcgcgctac
     2221 ctgatccgcc agcagcacag gcagcccatg ccggaggccg gtgtgcgcga ctcgatcgat
     2281 accatctccg gcaaaccgcg tcatggcgaa accgacagca gcgtccgtga caggcgcctg
     2341 accttcgaca tgctcgacga gccgcgcctg atcaccgcct acagcgccat cgagacactg
     2401 gccatgtttg ccgcctgcct gaccgcggcc gacctggtga gtggctggac caagggcagc
     2461 gccatcgagc tgcccacctt catctgggcg ctggccagtg gcgtggtgat tcgcaacgtg
     2521 ctgacccatg ccttccgctt cgagatgttc gaccgcgcca tcgacgtgtt cggcaacgcc
     2581 tcgctgtcgc tgtttctggc catggccctg ctcagcctgc gcctgtggga gctggccgat
     2641 ctggccggtc cggtggccat catcctggcc gtgcaggtgc tggtgatgat ggcctgggcg
     2701 tcgttcgtga cctaccgctt catgggggcc gactacgacg ccgcagtggt ggcggccggc
     2761 cactgcggct tcggcctggg cgccacgccg acggcgatcg ccaacatgca ggccatcacc
     2821 gatcgctttg gcccgtcgta caaggcctat ctggtggtgc cgctggtggg ggccttcttc
     2881 gtcgacctgg tgaacgccac ggtgctgcaa atcttcacgc agttgccgtt tctgcgttag
     2941 ggccggttca cgccatgcaa ggcgccccct tgctggcttc aatgggtgct ggacgagcca
     3001 cggccgacca cggccacgct gcggcggtga tcgacccagg tcaggcgccc gacgtcgaag
     3061 ccggcggcac gcgcgcgatc gagcaggcgg gcccgcgtgg cggcacgcat cgtcgggctg
     3121 cgcgccagca gccacagctg gtcgtgcgtg gggccgcaga ccagggcgtg ctggtcggcg
     3181 tcgtcgatgt cgaacacggc gcggctggtg cgcaccggcc agaccaagct gaggtgcagg
     3241 tgcgcctcgc gcgacgggtg cgccgggcgc gcgcgtgccg tggtggtgcg ccagcgcccc
     3301 aggcgcgtgt cgaagccgcg ccgggcgatc tgcagcgtgc catcgggcag caggctgtgg
     3361 tgcaccacac cgtcggtcag cccacggtcc tgcggcgggt cgatgcgcgc gatttcgtac
     3421 cagcggccga gccagcgttc cagcctgaac ggcgtcaccg ggtgcacgcc gggcggcaga
     3481 ccggtctgct ggctcaggcg gtacagcgca taggcgccga ccccgagcag ggcaagggtt
     3541 ctggctctca tggtcagcgc ggcagcatag caggcgcgcg gctgcggcgg gcgtgcggcg
     3601 gcaccggtgc ggatgacacg aagttcgtgt tgcacgaaat tcgtgtacac tgcccgacat
     3661 gaagaacgtc accatcaccg tcgacgaagc cgtgctcgaa tgggcgcggg tggaggcggc
     3721 gcggcgcggc tccagcgttt cgcgcatggt gggtgagatg ctggccgaga agatgcgtca
     3781 ggaagacgcc tacgcccagg ccatgcgcag cgcgctgcgc ttcgagtcct ggggcgccag
     3841 cgacgggccg tacctgcggc tgagcgaggt cgaatgatgc cgcccctggt gttcgtcgac
     3901 acgccggtgt tcctgtacgc agccgacgag cgcgatgggc gcaagcaggc gctggcgcgc
     3961 gaatgggtgc gctggtgctg gagccggcgc agcggccgca tcagcaccca ggtgctcaac
     4021 gagctctacc acaacgccat cacgcgcttt cgcgcccgca tgccggtgca gcaggcgcgc
     4081 gcgcaggtgc gcaacctgcg tcagtggcag ccgccgcacc tggatacctt caccatcgac
     4141 ggcgcctggg cgctgcagga tcgccatgcg ctgggctact gggatgcgct gatcgtcgcc
     4201 tcggcccaac agcaaggctg tcagtacctg ctgtgcgagg acctgccgca tcagcagcgc
     4261 ttcgagagcg tgcaggtgat caaccccttc cgggtccgtc ccgacgaact gccccttgcc
     4321 gatgagcact gacacctcga cccccagccc gctggcccgt atccgccagg acgtgcagtc
     4381 catgcacgcc tacgcggtgc aggactcggc cggcctgctg aaactggacg cgatggaaaa
     4441 cccccatcgc ctgcctgacg ccctgcaggc cgagctgggt cgccgcctgg gccgggtggc
     4501 catcaaccgc tatcccggcg cccgcgtggc cgagctgggc gccgcgctgc acgcccacgc
     4561 tgcgccgccg gcgggttggt cgctgatgct gggcaacggc tcggacgagc tgatcagcct
     4621 gctggccatg gcctgcgacg tgccgggagc gtctatcctg gcgccgctgc ccggcttcgt
     4681 gatgtatgcc atgagtgcgc gcctgcaggg cctggcgttt cacggtgtgc cgctgacgcc
     4741 cgacttcgcg ctcgacgagg ccgccatgct ggccgccatc gagcgccacc gcccggccat
     4801 cctttacctg gcctacccca acaatcccac cggcaccttg tgggacgccg gcagcatcgc
     4861 ccgtctgatt gccgcccagg gccgccaggg cgggttggtg gtgatggacg aggcctacca
     4921 gccctttgcc gcgcacagct ggatcgaggt ggtgcgcgcc gcccccgagc cgcaccgcca
     4981 cgtgctgatc atgcgcacgc tgtccaagtt cgggctggcc ggcgtgcgca tcggctacct
     5041 gatgggcgag gcgacgctgc tggccgaaat cgacaaggtg cgcccgccct acaacgtcag
     5101 tgtgctcaac gccgagtgcg cgctgttcgc gctcgaacac gccgacgtgt ttgccgcgca
     5161 ggcggccgac atccgcgcgc agcgcgcgca gctgctgcag gcgctggcgg cgctgccggg
     5221 tgtcacgccg tttcccagcc aggccaacat ggtgctggcg cgcgtgcccg atgcggcgcg
     5281 cgtgtttgcc ggcatgcgcg cgcgcggtgt gctgatcaag aacgtttcta caatgcacgc
     5341 gctgctggcg aactgcgtgc gcctgacggt cggtaccgcc gacgagaacg cgcagatgct
     5401 ggcggccctg caagcctcgc tatgaccacc actgcacgca gcgccgacgt cgcgcgcgac
     5461 accgccgaaa cccgcatccg tgtgcgcctg aacctggacg gcagcggcca ggccacgctg
     5521 gccaccggca tcggtttttt cgaccacatg ctcgaccaga tcgcgcgcca cggcctgatc
     5581 gatctggacg tgcagtgcca gggcgatctg cacatcgacg gccaccacac ggtggaggac
     5641 gtgggcatcg ccatcggcca ggcggtgcgc caggcggtgg gcgacaagcg cggcatcacc
     5701 cgctacggcc acagctacgt cccgctggac gaagcgctgt cgcgcgtggt gatcgatttt
     5761 tcgggtcgcc ccggcctggt gctggacgcg cgctttgcca gcgccatgat cggcggcttc
     5821 gacacccagc tggtgcatga attctttcag ggcttcgtca accacgccca ggtcacgctg
     5881 cacatcgaca acctgaaggg cgtgaacgcg caccaccagg tcgaaaccat cttcaaggcc
     5941 tttggccgtg cgctgcgcat ggcgctcacg ccggatgcgc gcgccgccgg tcaggtgccc
     6001 tcgaccaagg gcacgctcta agtcaaatcg gcctccagcg cttgacagat aagcgccaac
     6061 agctatgtta tcgatagcaa atcaaacggt cgccgtggtc gactacggca tgggcaacct
     6121 gcgctcggtg gcgcaggcgg tgatgcacgc cgcgcaggac acgcgcgtgg aggtggtggt
     6181 caccgacgat ccggcgcgcg tgcgcgcggc gcaccgcgtg gtgctgccgg ggcagggcgc
     6241 gatgggcgac tgcatgcacc agctgcatgc ctgcggcctg ctcgaatcgg tgctggaggc
     6301 cgccgacggc aagccgctgt tcggcgtctg catcggcatg cagatgctgc tgacgcacag
     6361 cgccgagggc cacaccgccg ccggcacacc cggtctgggc ctgatcccgg gcgaggtgct
     6421 gcgcttcgat ctggccggtc gccaggcgcc cgacggcagc cgctacaagg tgccgcacat
     6481 gggctggaac cgggtgttcc agagccgccc gcacccgctg tggcagggcg tggccgacgg
     6541 cgcctggttc tatttcgtgc acagctactt tgcccgcccg cacacgccga cccatgccgt
     6601 gggcgaaacc gagtacggcg cacgttttgc cagcgccgtg gcgcgcgata atatttttgc
     6661 tacccagttc cacccggaaa agagtgccga ccagggcctt accctgtacc gcaacttcct
     6721 gaactggcgg ccctgaacct ggcgcggcgt tgtgcgcgac gcgttctgca cgcaccgccc
     6781 agcccggccg atcgacttct tcatcgttca ttcgttccat ctgtcccgaa catgttgctg
     6841 atccccgcca tcgatctcaa ggacggccat tgcgtgcgcc tgaagcaggg cgacatggac
     6901 caggccacgg ttttcagcga ggccccggcc gagatggcgg cgcactggct gggccagggc
     6961 gcgcgccgcc tgcatctggt ggacctgaac ggcgcctttg ccggcaagcc gcagaacctg
     7021 gcggccatca aggcgatcct gcggcaggtc gatgacgaaa tcccggtgca gctgggcggg
     7081 ggcatccgcg acctcgacac catcgagcgc tacatcgacg ccggcatccg ctacgtcatc
     7141 atcggcacgg cggcggtcaa gaacccgggc tttctgaagg acgcctgcac cgccttcggt
     7201 ggccacatca tcgtcggtct ggacgccaag gacggcaagg tcgccaccga cggctggagc
     7261 aagctcaccg gccacgaagt gatcgacctg gcgcgcaagt tccaggactg gggcgtggaa
     7321 agcatcatct acaccgacat cggccgcgac ggcatgctca gcggcatcaa catcgaagcc
     7381 accgtcaagc tggcgcaggc gctcaccatc ccggtcatcg cctccggcgg gctgtccacc
     7441 atggccgaca tcgagcagct gtgcgcggtc gagagcgaag gcatcgaagg cgtcatcgcc
     7501 ggtcgtgcca tctattccgg cgatctcgac ttcgcggccg cgcaggcgcg cgccgacgcg
     7561 ctgaacggct gaccccattc catgctggcc aagcgcatca ttccctgcct ggacgtgacc
     7621 ggcggtcgcg tcgtcaaggg cgtcaatttc gtcgaactgc gcgatgccgg cgacccggtc
     7681 gagatcgccg cgcgctacaa cgagcagggc gccgacgagc tcaccttcct cgacatcacc
     7741 gccaccagcg acgggcgcga cctgatcctg ccgatcatcg aagcggtggc cagccaggtg
     7801 ttcattccgc tcaccgtcgg cggcggcgtg cgcacggtgg acgacgtgcg ccgcctgctc
     7861 aacgccggcg ccgacaaggt cagcttcaac tcggccgcca tcgccaaccc gcaggtgatc
     7921 cgcgacgcct cgggcaagta cggcgcgcag tgcatcgtgg tcgccatcga cgcgcgccgg
     7981 cgcagcggcg acgagctggc cgcgcgtggt cccggctggg acgtgttcag ccacggcggg
     8041 cgcaggaaca ccgggctgga cgccatcgcc tgggcacgcc agatggccga ctggggcgcc
     8101 ggcgaaatcc tgctcaccag catggaccgc gacggcacca gaagcggctt cgacctggaa
     8161 ctgacccgcg cggtgagcga cgcggtgccg gtgccggtga tcgcctccgg cggcgtcggc
     8221 agcctcgaac acctggccga cggcgtgcag ctcggcggcg ccgacgccgt gctggcggcc
     8281 agcatctttc actacggcga attcaccgtc gcccaggcca agcagcacct ggcggcgcgc
     8341 ggcattccgg tgcgcctgtg acgcccgcgg gcgcttgcga acaccgccga tgaacacctt
     8401 tgccgtgcgt caggaaaccg ccgctggcag ccgttatgat ccacggccca cggtccgcgc
     8461 atgccccggc atgcccaagg cggacgcggg ccgcgatcga acgccggttc gacgcaccca
     8521 cccatcccat ccaaggagcc catgttgagc accgccgcga gcctgaacga ctttctgcgc
     8581 cgtatcgaac agcgcgaccc ccagcaacca gagttcctgc aggcggtgcg cgaggtcatg
     8641 atgtcgctct ggccgttcct ggagaaacac ccccggtacc gcgaacacgg cttgctggag
     8701 cgcctgtgcg agcccgaccg cgtgatccag ttccgcgtcg cctgggtcga tgacaagggg
     8761 cagacccagg tcaaccgggc cttccgcgtg cagcacaaca tggccatcgg cccctacaag
     8821 ggcggcatgc gctttcaccc cagcgtcaac ctgtcgatcc tgaagttcct cggcttcgag
     8881 cagaccttca agaacgccct caccaccctg cccatgggcg gcggcaaggg cggcagcgac
     8941 ttcgacccca agggcaagag cgacggcgag gtgatgcgct tttgccaggc cctgatgagc
     9001 gagctgtacc gccacctggg cgccgacacc gacgtgccgg cgggcgacat cggcgtcggc
     9061 gcgcgcgaag tcggcttcat ggccggcatg atgaaaaagc tcagcaacaa cgccggcagc
     9121 gtgttcaccg gcaagggcat cgcctacggc ggcagcctga tgcggcccga ggccaccggc
     9181 tatggcacgg tctacttcgc ccaggaaatg ctgcgccgca agcagatggg cttcgaagac
     9241 cggcgcgtca gcatctcggg ctccggcaac gtggcgcagt acgccgccga caaggcgatg
     9301 gaactgggcg ccaaggtggt caccctgtcc gactccgacg gcaccctggt gtgcgagggc
     9361 ggcctgacgc acggcatgct gcaggacctg atggaattca agaacgtgca gcggggccgc
     9421 ctgaaggact tctgcaagca gcacaagctc aagttcgagg ccggcaagcg cccctggcat
     9481 gtgccggtgg acatcgcgct gccctgcgcc acccagaacg aactcgatgc ggccgacgcc
     9541 aagcacctga tcgccaacgg cgtggtgtgc gtggccgaag gcgccaacat gcccagcacg
     9601 ctggacgcgg tcgagcattt cctggccgcc ggcaccctgt acgcgccggg caaggccagc
     9661 aacgccggcg gcgtggccac ctcgggcctg gagatgagcc agaacgccat gcgcctgtcc
     9721 tggacccaca gcgaggtcga tctgcgcctg cacgagatca tgaaggacat ccacggcaac
     9781 tgcgtgcgcc acggcgagcg caaggacggc accgtcaact acgtcgaagg tgccaacgtg
     9841 gccggcttcg taaagatcgc cgacgccatg ctggcgcagg gcgtctgctg accacctcct
     9901 ggcgcggcgc gcctgcgccc tcaaggggcg tcgcccgagg ccgagggctc cggccgcgtg
     9961 cccgatgacg catgccgggt gaccgcgcgg cgcctggatc aggggcaggt cggacatcgt
    10021 gtcggcgccg ggcatcaccg acaccatcgg agttcaacct gggctcaagg caaatcggcc
    10081 tctggcgctt gcccatcaag cgccaacagc tatcaacttt gtagtttctg cggccaccag
    10141 gcgaatgccc gacaatcgcg gcatgagcaa ctggttggat caagtcgcct gggacgcgca
    10201 gggcctggtg ccggcgatcg cgcaggaagc ggacacgggc gacgtgctga tgttcgcctg
    10261 gatgaaccgc gaagcactgg ccaggacggc cgaactcggc cgggcggtgt atttcagccg
    10321 ttcgcgcggc aggttgtggt tcaagggcga ggagtccggc catgtgcagc aggtgcatga
    10381 aatccgcatc gactgcgaca atgacgtggt gctgctcaag gtgacgcagc agggccacag
    10441 gcccggcatt gcctgccaca ccgggcgcca cagctgcttc ttcagcgtgc tgaaggacgg
    10501 cgcctggcag gccgtcgagc cggtcttgaa agaccccgag tccatctata aataaggccc
    10561 catgtcgcac gccgagcttc tggcccatct gaccgccgtg atcgaaagcc gcaagccggc
    10621 ggccggcggc gaccccgaca ccagctacgt cgcgcgcctg ctggccaagg ggcccgacgc
    10681 cttcctcaag aagatcggcg aagaagccac cgaggtggtg atggccgcca aggacgccga
    10741 ccacggcggg ccgcgccaca aggtggtcag cgagatggcc gacctgtggt ttcacggcat
    10801 ggtggcgctg gcgcactacg ggttttccgc cgccgacgtg gtggcggaac tggcgcgccg
    10861 cgagggactc tcaggcctgg aagaaaaggc gctgcgcaag gcgcgccagc gcgacatcga
    10921 tgacgcttca cgcgaaggag ctggaacatg agccgcaacg acatcattga cgtggaaccc
    10981 gtgggcagcg cacgcgacga ctcgctcaag acctggggct gggtcagcta cctgctgcac
    11041 ctgatcgtgg cggtggcggc ggtggtgccg ggtgcgcagg tcagcattgc gctgctgatc
    11101 atcgcgctgg tgctggacct ggtgaagaag gacgatgccg ccggcacctg gcaggccagc
    11161 cacttcagct ggcgcatccg ctccgtgctg tgggccggcg tgctgtacct ggtcaccttc
    11221 ccgctgttcc tgctgttcta cctgccgggg gcgatcgcct gggtggtgat ctcgatctgg
    11281 ttcctctacc gcatcgtgct gggcatggtg cgcatgaacg ccgggcgccc ggtcgaggcg
    11341 taagcgcgcc gcccgaacgg cgcgcctgtg tcacgcccat cgaacccgca tggcgtacca
    11401 cttgatgacc ctggccaccg gctccggcac gaccgcagcg cgcacggcca tccgggcgca
    11461 gacgggccgt tcatcatccg tcagggcgtg tccccgcgcc gtcgcaccgc cgggggttct
    11521 gccattgcca acccactgtc accgatgtct tccatgagcg ctcacgccga cccccactgc
    11581 atcttctgca agatcgttgc cggacagatt ccatcgcgca aggtgtacga ggacgacgag
    11641 ctgtttgcct ttcacgacat ccacccctgg gcgccggtgc acttcctgat ggtgcccaag
    11701 ctgcacgtcg cctccatggc ccatgtgggg ccggagcacg aagcgctgct gggccgcatc
    11761 atggcgctgg cgcccaagct ggcgctggaa cagggctgcc agccgtaccc tgacggcggg
    11821 tttcgcatcg tggtcaatac cggggctcag ggtggccagg aagtgcatca tctgcacgtg
    11881 cacgtgatgg gcgggccgcg cccgtgggcg cgcgggtgaa gcggccgccg gcgagtggcg
    11941 gcgcaagccc gcgccggggt gtaccggtcg tggccggcaa cgggcatgat cgcgctggaa
    12001 ccgggcccat ccccggacgt ctaaaatcat tctgattcgc gcccgttcat cggtcggcgc
    12061 aaggagttgc aacatgggta ctttttcgat ctggcactgg ctgatcgtgc tgctgatcgt
    12121 ggtcatgatc ttcggcacca agaagctcaa gaacatcggc tccgacctcg gcggtgccgt
    12181 caagggcttc aaggacggca tgaaggacgg tgcggccgag gccgacacgc cgccggccgg
    12241 tcaggtgacc aacgccgccg cggccaagga caccatcgac gtgcaggcca aggaaaagag
    12301 ctgagccggc ccgccgcgtg cgcgcggggc gtggccgaca cgcccgacgc tggctgcccg
    12361 cgaggctgaa cccttcagat gctcgatctc ggtatttcca agatggcgct gatcggcgcc
    12421 gtggcgctga tcgtcatcgg ccccgaaaag ttgccgcgcg tggcacgcac ggtgggcacg
    12481 ttgctgggca aggcccagcg ctacgtggct gacgtcaagt ccgaggtcaa tcgtgcgatg
    12541 gacctcgacg agctcaagaa gatgaaggag tcggtggaat ccgccggccg cgaagtcgag
    12601 cagagcatcc atgccaccca gtcggcgttc gagaaggact ggaacgaggc caccgccggg
    12661 ctgggcggta ccggcacggg cgaggacgcc ccgatcccgt cctgggagcc ggcgccgccg
    12721 caataccgac gccccgacaa gaactggcgc ctcaagcgcg gcgccacgcc gcagtggtac
    12781 aagcagcgcc acggcgtgcg ccggcaggtg cagtcgggcg cggcgcgcgt ggcgcgctat
    12841 cgccccaaga gtccatgacc catgaccgtt gaccctgccg cagcgcggca tcgatgacgc
    12901 caggccggca tcggccatgc atcgttgaag ccgcccccgg cggcacagtc gctcgtcatg
    12961 acacccctac caccatgtcc aaccccacgc ccgaccccga agacgaactg gccggcaccg
    13021 agcagccatt cgtcgtccac ctgatggagc tgcgcgatcg gctgatcaag tcgatggtgg
    13081 cggtggctgt ggtggcgggg gcgctggcgc tgtatccggg gccgggcgcg ctgtacgacc
    13141 tgctggcggc gccgctggtg gcgcacctgc cgcagggcgc gacgatgatc gccacctcgg
    13201 tcatctcgcc cttcatggtg ccgctgaaga tcctgctgat ggcggccttt ctggtggcgc
    13261 tgccggtggt gctctaccag gtgtgggcct tcgtggcgcc gggcctgtat tcgcatgaaa
    13321 agcggatggt gctgccgctg gtgatctcga gcacggtgct gttcttcgcc ggcgtggcgt
    13381 tctgctactt tctggtgttc ggcaaggtgt tcaccttcat ccagagcttc gcgcccaagt
    13441 cgatcacggc ggcgcccgac atcgaggcct acctgagctt cgtgctcacc atgttcctgg
    13501 ctttcgggct ggcgttcgag gtgccgatcg ccgtcgtggt gctggcccgc ctgggggtgg
    13561 tgagcatcga gcagctcaag agctttcgcg gctatttcat cgtgctggcc ttcatcattg
    13621 cggccatcgt cacgccgccc gacgtggtgt cgcagctggc gctggccatt ccgatgtgtc
    13681 tgctgtacga ggtcggcatc tgggccgcgc gcctgttcat caagcacacc accgcacccg
    13741 aagatgccgc acccgccgcc gggccctgaa gcgcgaccca gccggacaag gggcaaatcg
    13801 acctccagcg cttgtcaggc aagcgccgtc agctatcgtt tttgattcat cgtggcagcg
    13861 cgggctgccg tggccgcggg cgctgggccg gggtgacgga tgcctgcacc gcctcgccgc
    13921 ggcgctccag cgtgaacggc accgcttcgc cgggcaccag tgcggctatg cgcgtcagca
    13981 gttcggccac ggtgcgcgtg ggctgaccgc ccacctgggt gatgacatcg cccggccgga
    14041 tgccggcgct ggccgccggg ccgttgccca gcacgccggt gatgatcacc ccctgctcgg
    14101 ccttgacgcc gaaggtctgc gccagctcgg gcgtcagctc gcccggctcg acaccgatcc
    14161 agccgcgcac cacctggccg ttgcggacga tgctttccat cacctggcgc gccgtcgaca
    14221 ccgggatggc aaagccgatc cccatgttgc cgcccgagcg cgaatagatg gcggtgttga
    14281 tgcccatcag gtggccgccg atgtcggtca gcgcgccgcc cgagttgccg gggttgatgg
    14341 cggcgtcggt ctggatgaag ttctcgaagg tgt
//
LOCUS       DAOQTZ010000072        14208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4831_length_14208_cov_12.949057, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000072 DAOQTZ010000000
VERSION     DAOQTZ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14208
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4831_length_14208_cov_12.949057"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /locus_tag="PKN51_09330"
     CDS             <1..1596
                     /locus_tag="PKN51_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNT85339.1"
                     /translation="SAGCVLAARLSEDPSVQVTLLEAGPPDRSALIHCPAGLALMAKS
                     GQVNWGFESVPQPGLNGRRGFLPRGKVLGGSSSVNAMIYTRGVPQDYDAWAAEGNAGW
                     GWDEVLPFFRRAEHNERGADAFHGGEGPLNVKDLAEPNPFSHAFVEAGVQAGYRHNTD
                     FNGADQEGVGLYQVTHKAGERFSSAKAYLTPNRARPNLRVITGAHATRVLLEHGRAVG
                     VAYLQDGREQIVRAEREVIVSAGALQSPQLLLLSGIGPADPLRQHGIAVAHELPGVGE
                     HLHDHPDVVQLWSVPGAHDLFGLSAVGLWRAARGAWQWQRERRGMLTTNFAEAGGFIK
                     SDASEPLPDLQLHFVVGKLVNHGRTTVFGHGFSCHVCVLRPRSRGSLRLASSDPLAAP
                     RIDTALLQDADDMRRMVRGFKLMRELLSQPALARHGGRESSADRRSDAEIEGFIRQYA
                     DGIYHPVGSCRMGHGPMDVVDERLRVRGIDGLRVVDASIMPSVVAGNTNAPVIMIAEK
                     AATLIRESANPAGNPDRSGVKPG"
     gene            complement(1623..2999)
                     /locus_tag="PKN51_09335"
     CDS             complement(1623..2999)
                     /locus_tag="PKN51_09335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT85340.1"
                     /translation="MKSSILVVEDDPVLNGLLVKELRKAGYDIDSASSWRDAQQRIAA
                     FAPNLVLLDVNLPDASGFGPLSEIAASRPVVMLTAYGNVNQAVEAMRLGAADYLVKPV
                     NLDELELVIRGALERSQLRAQKALGAGDAASSAMIGDSPAMRQLRKTIAEVALSDVTV
                     LIEGESGAGKELVAQALHAASARRSEPFVPVDCCTLQESLFESELFGHERGAFTGADR
                     RKAGLIEAAAHGTLFLDEIGEAGPAIQAKLLRVLETGRFRHVGATTDLRTDARIVAAT
                     NRDLRERAREGQFRDDLYYRLSAFVLRVPPLRARREDIGALAAHFIARRRQGRGLPPI
                     RLSPAALEHLTRYDWPGNVRELRNVIERALILVGNAPEITPAHLGLVEPAPGGATAAT
                     PGPAVAVTSSGEETLVLRGEPTLDSIEHEYLGQLLQKYGGNRRKVAEALGVSERTAYR
                     MMERHGYK"
     gene            complement(3013..4959)
                     /locus_tag="PKN51_09340"
     CDS             complement(3013..4959)
                     /locus_tag="PKN51_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT85341.1"
                     /translation="MNSAPAAHMPGAAPTAAPRSGLGAWLGASLARRLTLGLIVALLL
                     SAGLLVALALAISRDALRREHEQSATRVAHLFEASLQNAMLKHDIEGMAGILAALGHS
                     PGVAGARLLAPQGTVRFASRPEAVGSSAQAQIAGLCLHAGCTPSAPRLAWHAAPEGDR
                     LQIAYPILNQARCAQCHGPAAERPVNGVLLVDFKPLPPATPGSGPALLLAGAGLAALG
                     VFAAIMGFTLRRQVTQPLAALAQATDRLAAGDLSARVNASGDDELGRVSHGFDRMAAR
                     IDAMVGRLDAQARFLQTLIDAMPDPVLVIGADHRIRLANQAYATLLGHDAQAVVGQPC
                     YRASRGLAEPCPSTLVTCPLAEIRQHAQPVRSMLRLQRSDGAPVEVDIEAAPVHAADG
                     ETLVVEVLRPLERALRFSQEQRLSTIGLLANGVAHEIHNPLASIRLALQAILRGLRNG
                     GMDRPEMVDYLELVDREIDRCVLTTQRLMQMSQAPTQALQPVRLVTAIDDVLALLREE
                     CRARGVQVRVSGVPESERVRGDEAELRQVLVNLVHNALHAMPQGGRIDIEGAASDDGA
                     RLRLAVRDSGCGIALADQPLIFMPFFSRRVDGQRGTGLGLAICKATMERFGGAIEVQS
                     TPGQGSTFTLTLQRVTADAVTGVG"
     gene            complement(4956..6014)
                     /locus_tag="PKN51_09345"
     CDS             complement(4956..6014)
                     /locus_tag="PKN51_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT85342.1"
                     /translation="MNHLDIHAERCVHQQVVASDCDACVRACPPGAWRLHDDGLGFDA
                     DACDGCGLCVAACPTGALELPAPQALLHTDARGKRSLLLACERADVHGAAGVVACLYA
                     LAPGWLLRQCDTHRAHAIAVAPGDCTHCERGAAGPAWRAHWLAVAARLPAAPQLKPTT
                     APEWTARASAPAVPDAGRRAFFRRLAPVATRATATASEPALSSARADTVARLAVVGTD
                     APPAPLWAVTLDTARCTFCMACTRLCPTGALAVELGSAVEQFRLDMARCTGCGLCQAV
                     CGDGALSAPRTPAPDDTALAQPVRLPLHRRRCPSCRIDFHQFAAPAEGPHSEERCPTC
                     RQGRPWQPKLVVQDATPS"
     gene            complement(6109..6801)
                     /locus_tag="PKN51_09350"
     CDS             complement(6109..6801)
                     /locus_tag="PKN51_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNT85343.1"
                     /translation="MNAPHPPWQALAAEDLRALAWLHTQERPPEVLAALYASGFPATL
                     TLVGADAAEAQAMDAALRALASSRCEEDAPGALPASSADELAADYAAIYLTHALRASP
                     HESVWRDDDHLMLQGPTFAVRAFYRRHGIQVSDWRARADDHLVHELEFVALLLERGDE
                     REAARFLKTHLLAWLPDFARRVAQRAATPFYAALAGLTLAACEACQRRLPSVAALPPV
                     VADGPAGAPGCG"
     gene            complement(6798..7532)
                     /locus_tag="PKN51_09355"
     CDS             complement(6798..7532)
                     /locus_tag="PKN51_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT85344.1"
                     /translation="MAEKQVINKLGVDAKARVKYAQEKRDATTYEPVVPQQQWGFIHN
                     NMDCIGCRACEIACKDKNGLPPGPRFRRVMYIEGGSFPDVFAYKVNMSCNHCAEPACL
                     PSCPTGAIWKRADNGVVDIDSTLCIGCRRCEATCPYGAPQYDPTDNTVKKCNLCIDEL
                     EAGRKPYCVSACMMRVLDIGPIDQLRAGTWDTKAMGPGDKPVRQVQHMANPELTNPSI
                     VFIAHPKGKVEPPPQTPAAPAPKASS"
     gene            complement(7545..10169)
                     /locus_tag="PKN51_09360"
     CDS             complement(7545..10169)
                     /locus_tag="PKN51_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT85345.1"
                     /translation="MKHDLPEVLDAPAAARFPSVNDSIDQHSTGYKRRDFLKIVGATA
                     GAAGLVSVGVGIAPEKAQAFAYEPYFTDDQLTTVVTSCAHNCGSRHVLVAHKKGDVIV
                     RLSTDNGEYQAQGAYGKDTFEEPQLRACLRGRSYRARLYSPERLLYPMKRVGARGEHK
                     FKRISWDEALNEIAQKMVQLKKDFGPTAILDQSYAGASYGVLHKSDQIEGLLGRFLGM
                     FGCRTNSWSVPSYQGTTFSSRMTFGTIQDGNEDDTFAHSKLIIMWGWNPAYTFHGGNT
                     FYYMRMAKQKGCKFVLIDPQYTDSAAVYDAWWIPIKPNTDAAMMAGMAHHIFTNNLQD
                     QKFINKFCLGMDAGTTTGDYKGKENFKDYILGKYDNTPKTPEWAAAICGVKAEDIIKL
                     AEMYAKTKPAALKASWAPGRASYGEQYNRMAAALQAMTGNIGVLGGCAEGVGKAWHSE
                     SVAYPYDENANLWWASIKSDRWAHCVLNYPDIKREELGLWPREDDLDGKVPNIKAIFW
                     QGSDWFNQLTNINKEIKAIEKLELVVCMDSTITPSGIWADYLLPIATHFERHDVALPW
                     YKGHYYIHRPKVIEPLGESKTDFQVFTELSYRLEELDPNLKNLGKRYNPRADRSYFQN
                     PDAVDEAYLLAWWTNNVQHHQGVTMSWDEFKKHGTYKFKLKEPHVAFREQVTKGVPFE
                     TPSGKIEILSTQLADITDWTKTQYGYEIPYIPKWVEPFESLNHPIAKQYPFHLISPHP
                     RWRTHSIFNNIPWLRETFEQEVTINASDAKKLGIQTGDTVEVWNDRGKCVVPAYVTER
                     LLPGVLVLFEGAWMDPDKDGIDRAGNPDFLTLDNPSPAGAFAYNNAMVQIKKTDLVHR
                     PGWDDLAVARSSVFRRDM"
     gene            complement(10166..10999)
                     /locus_tag="PKN51_09365"
     CDS             complement(10166..10999)
                     /locus_tag="PKN51_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85346.1"
                     /translation="MAAWLASGCSDNAKPITPAPVASAPPAAAPAPAAVKPVSYHAAK
                     YDPIHFKPAIEKATDQQCLACHAEVLKPSVRKTSPAGLPASTAKAWYQVTSTYQGEQD
                     TFHRRHLETPLAKQVMNMRCTTCHEGNDPREEAPGSSTFDSGQQILRKMVNPETVCLK
                     CHGKMNVEVMGLPGPWEQSKATFQNNCLLCHAGIRTTRHQVNYLNAKAIEELGAKPNG
                     GDLCFGCHGGRSWYRTHYPYPRHAWPGMAADVPDWAKGRPTESEVRFISAAVAQTGDK
                     K"
     gene            complement(11177..>11569)
                     /locus_tag="PKN51_09370"
     CDS             complement(11177..>11569)
                     /locus_tag="PKN51_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85347.1"
                     /translation="GMSGMSGQQGMSGMSGMSGMSGQKGMSGMSGMSGQKGMSGMSGM
                     SGNKAKAAKKKKKPRKAGQQGMSGMSGMSGMSGMSGMSGMSGMSGMSGQQGMSGMSGQ
                     QGMSGMSGQQGMSGMSGQQGMSGMSGSK"
     assembly_gap    11570..11669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11670..11964)
                     /locus_tag="PKN51_09375"
     CDS             complement(<11670..11964)
                     /locus_tag="PKN51_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85348.1"
                     /translation="MKEISMKLVRKALGLSVMAAALAALPAMAQQSGMSGMSGMSGNK
                     PAAGEQNPGMSGMSGMSGQQGMSGMSGMSGQQGMSGMSGQQGMSGMSGMSGQQG"
     gene            complement(12120..12494)
                     /locus_tag="PKN51_09380"
     CDS             complement(12120..12494)
                     /locus_tag="PKN51_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85349.1"
                     /translation="MKPILVVTAGLLAVLTAAQAQAPGASAPAVATPVAGAPIAGLQP
                     HQRPAGAPTETDQPASPEQMARWLRGVSAPHPGNVDTIAATGRWWVPLRGPGMTPPYD
                     PRGWHEGASAVSPVAPASAASR"
     gene            complement(12748..13557)
                     /locus_tag="PKN51_09385"
     CDS             complement(12748..13557)
                     /locus_tag="PKN51_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT85350.1"
                     /translation="MANRNEASVWAPPRLSMRWWPVFQRNLLVWRKLALPSLLANIGE
                     PLLWLVAFGYGLGAMVGRVPTHGQEVPYIVFLASGSICMSAMNAASFEALYSAFSRMH
                     VQKTWDGILNTPVQLDDVVLAEMLWAGFKALFSVVAILGVMLALKISLSPKLVLVLPI
                     GLVAGVTFASMALVFNAKAQGYDFFTYYFAVLLSPMMFLSGVFFPRSQLPGFAQTITD
                     WLPLTAAVELVRPLFLDQWPADAARHLAVLAAYAIASFWIALAFTRQRFRR"
     gene            complement(13550..>14208)
                     /locus_tag="PKN51_09390"
     CDS             complement(13550..>14208)
                     /locus_tag="PKN51_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT85351.1"
                     /translation="DPDFSCAENLFVFGRYFGLTRSQMAERVPQLLDFAALGHKADAK
                     PAELSGGMKRRLSLARALVNNPDLLLLDEPTTGLDPQARHLMWERLQRLLAEGKSILL
                     TTHFMDEAERLCHRLLVLDHGRKIAEGRPRELIAEHLEPEVIEVYGHGALALADSTLA
                     AHAERVERSGETVFFYTRHARPLMQALEAHPQLRTLHRPANLEDLFLKLTGRQIRQDG
                     "
BASE COUNT         2122 a         4655 c         4982 g         2349 t
ORIGIN      
        1 tcggccggct gcgtgctggc cgcgcgcctg agtgaagatc ccagcgtgca ggtgacgctg
       61 ctcgaggccg ggccgcccga ccgcagcgcg ctgatccact gcccggccgg cctggcgctg
      121 atggccaagt ccggccaggt caactggggc tttgaatcgg tgccgcagcc gggcctgaac
      181 gggcgccgtg gctttctgcc gcgcggcaag gtgctggggg gcagcagttc ggtcaatgcc
      241 atgatctaca cgcgcggcgt gccgcaggac tacgacgcct gggccgccga gggcaacgcg
      301 ggctggggct gggacgaggt gctgcccttc ttccggcggg ccgagcacaa cgagcgtggc
      361 gccgacgcct tccacggtgg cgagggtccg ctcaacgtca aggatctggc cgagccgaac
      421 ccgttttcgc acgctttcgt cgaggccggc gtgcaggccg gttaccggca caacaccgac
      481 ttcaacggcg ccgaccagga gggcgtgggc ctgtaccagg tcacgcacaa ggccggcgag
      541 cgtttctcga gcgccaaggc ctacctgacg ccgaatcgcg cgcggcccaa tctgcgcgtg
      601 atcaccggtg cgcacgccac gcgcgtgctg ctggagcacg gccgcgcggt cggcgtggcg
      661 tacctccagg acgggcgcga gcagatcgtg cgcgccgagc gcgaggtcat cgtcagcgcc
      721 ggggccctgc aatcgccgca gttgctgctg ctgtcgggca tcgggcccgc cgatccgctg
      781 cgccagcacg gcatcgcggt ggcgcacgag ttgcccggcg tcggcgaaca cctgcacgac
      841 caccccgacg tggtgcagct gtggagcgtg cccggcgcgc acgacctgtt tggcctgtcg
      901 gccgtcggcc tgtggcgcgc tgcgcgcggc gcctggcaat ggcagcgcga gcggcgcggc
      961 atgctgacca ccaacttcgc cgaggccggc ggcttcatca agagcgatgc gtccgagccg
     1021 ctgccggatc tgcaactgca tttcgtcgtc ggcaagctgg tcaaccatgg ccgcaccacc
     1081 gtgttcgggc atggattttc ctgccatgtg tgcgtgctgc ggccacgcag ccgcggcagt
     1141 ctgcgcctgg cgagcagcga ccccctggcg gcgccgcgga tcgataccgc gctactgcag
     1201 gacgccgacg acatgcgccg catggtgcgc ggcttcaagc tgatgcgcga gttgctgagc
     1261 cagccggcgc tggcgcgcca cggcggccgc gagagctcgg ccgaccggcg cagcgacgcc
     1321 gagatcgagg gttttatccg ccagtacgcc gacggtatct accacccggt cggcagctgc
     1381 cgcatggggc atgggccgat ggacgtggtg gacgagcggc tacgcgtgcg cggcatcgac
     1441 ggcctgcgcg tggtggatgc ctcgatcatg cccagcgtgg tggcgggcaa caccaacgcg
     1501 ccggtgatca tgatcgccga gaaggcggcg acactgatcc gcgagtcggc gaacccggcc
     1561 ggaaacccgg atcgatcagg cgtcaagccg ggctgaaacg gccggaccgg cggcgttgcc
     1621 cactatttgt agccgtggcg ctccatcatg cggtaggcgg tgcgctcaga cacgcccagg
     1681 gcctcggcca ccttgcggcg gttgccgcca tacttctgga gcaactggcc gaggtattcg
     1741 tgctcgatgc tgtcaagcgt gggttcgccg cgcagcacca atgtttcctc gcctgacgac
     1801 gtgacggcaa cggcgggacc tggggtcgcg gcggttgcgc cgccgggcgc cggctcgacc
     1861 aggcccagat gggctggtgt gatctcgggc gcgttgccga ccaggatcag cgcgcgctca
     1921 atcacgttgc gcaattcgcg cacgttgccg ggccagtcgt accgagtcag atgctccagc
     1981 gccgccgggc tcaggcgaat cggcggcagc ccccgaccct ggcggcggcg ggcgatgaaa
     2041 tgcgccgcca gcgcgccgat gtcctcgcgc cgcgcgcgca atggcggcac ccgcagcacg
     2101 aaggccgaca ggcggtagta caggtcgtcg cggaactggc cttcgcgcgc tcgctcgcgc
     2161 aggtcgcggt tggtggcagc gacgatgcgc gcgtcggtgc gcaaatcggt ggtggccccc
     2221 acgtggcgaa agcgcccggt ctccagcacg cgcagcagct tggcctggat ggcggggccg
     2281 gcctcgccga tctcgtcaag gaacagggtg ccgtgcgcgg cggcctcgat caggccggcc
     2341 ttgcgccggt cggcgccggt gaaggcgccg cgctcgtggc cgaacagctc ggattcgaac
     2401 agcgactctt gcagcgtgca gcagtccacc ggcacgaagg gttcgctgcg gcgtgcgctg
     2461 gcggcatgca gcgcctgcgc caccagttcc ttgccagcac cgctttcgcc ctcgatcagc
     2521 accgtgacgt cactcagcgc cacttcggca atcgtcttgc gcagctgccg catggcgggg
     2581 ctgtcgccga tcatggcgct gctggccgcg tcgcccgcgc ccagggcttt ttgcgcgcgc
     2641 agctgcgagc gctccagcgc gccgcgaatc accagttcca attcgtccag gttgaccggc
     2701 ttgaccaggt aatccgccgc gcccaggcgc atggcttcta cggcctggtt gacgttgcca
     2761 taggccgtca gcatgaccac cgggcgcgag gcggcgattt cgctcaacgg gccgaagccg
     2821 ctggcgtcgg gcaggttcac gtccagcagc accagattgg gcgcgaaggc ggcaatgcgc
     2881 tgctgcgcgt cgcgccacga actggcgctg tcgatgtcgt agccggcctt gcgcaactcc
     2941 ttgaccagca ggccgttgag caccgggtcg tcttcgacca ccaggatgga ggatttcatg
     3001 tcgacctcct catcaaccca cgcctgtgac cgcatccgcc gtcacgcgct gcagcgtcag
     3061 cgtgaacgtg ctgccctggc ccggcgtgct ctgcacctcg atggcgccgc caaaacgctc
     3121 catggtggcc ttgcagatgg ccagccccag gccggtgccg cgctggccgt ccactcggcg
     3181 gctgaaaaag ggcatgaaga tcagcggctg gtcggccagc gcaatgccgc agccgctgtc
     3241 gcgcacggcc aggcgcaggc gggcgccgtc gtcgctggct gcgccttcga tgtcgatgcg
     3301 gccaccctgc ggcatggcgt gcagcgcgtt gtgcaccagg ttgaccagca cctggcgcag
     3361 ttcggcctcg tcgccccgca cgcgctcgct ctcgggcacg ccgctcaccc gcacctgcac
     3421 gccgcgcgcg cggcactcct cgcgcagcag cgccagcacg tcgtcgatgg cggtgaccag
     3481 gcgcaccggt tgcagcgcct gcgtgggcgc ctgactcatc tgcatcaggc gctgggtggt
     3541 gagcacgcag cggtcgatct cgcgatcgac cagttccagg taatcgacca tctccgggcg
     3601 gtccatgccg ccgttgcgca acccgcgcag gatggcttgc agcgccaggc ggatcgaggc
     3661 cagcgggttg tggatctcgt gcgccacgcc gttggccagc aggccgatgg tggacaggcg
     3721 ctgctcttgc gaaaagcgca gcgcgcgctc cagcgggcgc agcacctcga ccaccagcgt
     3781 ctcgccatcg gcggcgtgca cgggtgcggc ctcgatgtcc acctcgaccg gcgcgccgtc
     3841 gctgcgttgc aggcgcagca tgctgcgcac cggctgcgcg tgctggcgga tttcggccag
     3901 cgggcaggtc accagcgtgc tggggcaggg ttcggccagg ccgcggctgg cgcggtagca
     3961 gggctggccg accacggcct gcgcgtcgtg gcccagcagc gtggcatagg cctggttggc
     4021 caggcggatg cggtggtcgg cgccgatcac cagcaccggg tcgggcatgg cgtcgatcag
     4081 cgtttgcaga aaccgcgcct gcgcatccag ccggccgacc atggcgtcga tgcgcgcggc
     4141 catgcggtcg aagccatggc tgacgcggcc cagttcgtcg tcgccgctgg cgttgacgcg
     4201 cgccgacaaa tcgcccgcgg ccagccggtc ggtggcctgc gccagcgcgg ccagcggctg
     4261 cgtcacctgg cggcgcagcg tgaaacccat gatggcggcg aacaccccca acgccgccag
     4321 ccccgcgccg gccagcagca acgccgggcc actgcccggc gtggcgggcg gcagcggctt
     4381 gaaatcgacc agcagcacgc cgttgaccgg gcgctcggcc gcggggccgt ggcactgcgc
     4441 acagcgcgcc tggttgagga tggggtaggc aatctgcagc cgatcgccct cgggcgctgc
     4501 gtgccaggcc aggcgcggcg ccgacggtgt gcagccggca tgcaggcaca ggccggcgat
     4561 ctgcgcctgg gcgctgctgc ccaccgcctc gggccgcgaa gcaaagcgca ccgtgccctg
     4621 cggcgccaaa aggcgcgccc cggccacgcc gggcgagtgc ccgagcgcgg ccaggatgcc
     4681 ggccatgcct tcgatatcgt gcttgagcat ggcgttttgc aagctggcct cgaacaggtg
     4741 cgccacgcgc gtggccgatt gctcgtgctc gcggcgcaag gcatcgcggc tgatggccag
     4801 ggccagcgcc accagcaggc cggccgacag cagcagcgcg acgatcaggc ccagcgtcag
     4861 ccgccgcgcc aacgaggcgc ccagccacgc gcccagcccc gagcgcggcg cagcggtggg
     4921 cgcggcgccg ggcatgtggg cggcgggtgc gctgttcatg aaggggtggc gtcctgcacc
     4981 acaagcttgg gctgccaggg gcggccttgc cggcaggtcg gacaccgttc ttcagagtgt
     5041 ggcccctcgg caggcgcggc aaattgatga aagtcaatcc gacaggacgg gcagcggcgg
     5101 cggtgcagcg gcaggcgcac cggctgggcc agcgcggtgt cgtcgggcgc cggcgtgcgc
     5161 ggcgccgaca gcgcaccgtc gccgcacacc gcctggcaca ggccgcagcc ggtgcagcgg
     5221 gccatgtcga gccgaaactg ctccacggcg ctgcccagct cgaccgccag cgcgccggtg
     5281 gggcacaagc gggtgcaggc catgcagaag gtgcagcgcg cggtatccag cgtgacggcc
     5341 cacagcggcg caggcggggc atcggtgccg accacggcca ggcgcgccac ggtgtcggcg
     5401 cgcgcgctgg acagcgccgg ttcactggca gtcgcggtgg ctcgggtcgc cacaggtgcc
     5461 aggcggcgaa agaaggcgcg ccggcccgcg tccggcacgg cgggcgcgct ggcgcgtgcc
     5521 gtccactcgg gggcggtggt gggcttcagc tgcggcgcag cgggcagccg cgccgccacg
     5581 gccagccagt gcgcgcgcca tgccggcccg gcggcgccgc gctcgcagtg cgtgcaatcg
     5641 cccggtgcca ccgcgatggc gtgggcacgg tgcgtgtcgc attggcgcag cagccaaccc
     5701 ggcgcgagcg cgtacaagca agccacgacg cccgccgcgc cgtgcacatc cgcgcgctcg
     5761 caagccagca gcaggctgcg cttgccacgg gcgtcggtgt gcagcagggc ttgtggtgca
     5821 ggcagctcca acgcaccggt ggggcaggcg gccacgcaca ggccgcagcc gtcgcaggcg
     5881 tcggcgtcaa agcccagacc gtcgtcgtgc aggcgccagg cgccgggcgg acaggcacgc
     5941 acacaggcgt cgcagtccga cgccaccacc tgttgatgca cgcagcgttc ggcgtggatg
     6001 tccaggtggt tcatggtgcg atactacata aacaatagct gcttgcgctg gatgggcaag
     6061 ggaaatcggc tcattatgct taaaaacagc cgcatctgcg ttgccctgtc aaccgcaacc
     6121 aggtgcgcct gcaggaccgt cagccaccac aggcggcagc gcggccacgc tgggcaaacg
     6181 gcgctggcag gcctcgcagg cggccagcgt caggccggcc agcgcggcgt agaacggggt
     6241 ggcggcgcgc tgcgccacgc ggcgcgcaaa gtcgggcagc caggcgagca gatgggtctt
     6301 caagaagcgc gcggcctcgc gttcgtcgcc gcgctccagc agcagggcca cgaactccag
     6361 ctcgtgcacc aggtggtcgt cggcgcgcgc gcgccaatcg ctcacttgaa tgccgtggcg
     6421 gcggtagaag gcgcgcacgg caaaggtggg gccttgcagc atcaggtggt cgtcgtcgcg
     6481 ccacaccgat tcgtgcggcg aggcgcgcag cgcatgcgtc aggtagatgg ccgcgtagtc
     6541 ggctgccaac tcgtcggcgc tggaggcggg cagcgcgccc ggcgcgtctt cttcgcaacg
     6601 gctgctggcc agggcgcgca gcgcagcgtc catggcttgg gcttcggcgg cgtccgcgcc
     6661 gaccagggtt agcgtggcgg gaaagccgct cgcgtacaag gcggccagca cttcgggcgg
     6721 gcgctcttgg gtgtgcagcc aggccagggc gcgcaggtct tcagccgcca gtgcctgcca
     6781 cggcgggtgc ggcgcgttca tgacgaggcc ttgggcgccg gcgcggccgg tgtctgcggc
     6841 ggcggctcga ctttgccctt cggatgcgcg atgaacacga tcgacgggtt ggtcaattcc
     6901 gggttggcca tgtgctgcac ctggcgcacg ggcttgtcgc ccgggcccat ggccttggtg
     6961 tcccaggtgc cggcgcgcag ctggtcgatg gggccgatgt cgagcacgcg catcatgcag
     7021 gccgacacgc aatacggctt gcggcccgct tccagctcgt caatgcacag gttgcacttc
     7081 ttgacggtgt tgtcggtcgg gtcgtactgc ggcgcgccgt aggggcaggt ggcttcgcag
     7141 cggcggcatc cgatgcacag cgtgctgtcg atgtcgacca cgccgttgtc ggcgcgcttc
     7201 cagatggcgc cggtcgggca cgacggcagg caggccggct cggcgcagtg gttgcacgac
     7261 atgttgacct tgtaggcaaa cacgtcggga aagctgccgc cttcgatgta catgacgcga
     7321 cgaaagcgcg ggccgggcgg caggccgttc ttgtccttgc aggcaatctc gcaggcgcgg
     7381 cagccgatgc aatccatgtt gttgtggatg aagccccact gctgctgtgg gacgaccggc
     7441 tcgtaggtgg tggcgtcgcg cttttcctgc gcgtacttca cgcgcgcctt ggcatcgacg
     7501 ccgagcttgt tgatgacttg tttttcagcc atgttcttct cccgtcacat gtcgcggcgg
     7561 aacaccgacg agcgcgccac ggccagatcg tcccagccgg ggcggtgcac cagatcggtc
     7621 ttcttgatct gcaccatggc gttgttgtag gcaaaggcgc cggcggggct ggggttgtcc
     7681 agcgtcagga agtcggggtt gccggcgcgg tcgatgccat ccttgtccgg gtccatccac
     7741 gcgccttcaa acagcaccag cacgccgggc agcaggcgct cggtcacgta ggcgggcacc
     7801 acgcatttgc cacggtcgtt ccacacctcc accgtgtcgc cggtctggat gccgagcttc
     7861 ttggcatcgc tggcgttgat ggtgacctct tgctcgaagg tctcgcgcag ccaggggatg
     7921 ttgttgaaga tcgagtgcgt gcgccagcgc gggtgcgggc tgatcaaatg gaacgggtac
     7981 tgcttggcga tggggtggtt gagcgactcg aacggctcga cccacttggg gatgtacgga
     8041 atctcgtagc cgtactgcgt cttcgtccag tcggtgatgt cggccagttg cgtcgacaga
     8101 atttcgatct tgccgctggg cgtctcgaac ggcacgccct tggtcacctg ctcgcggaag
     8161 gcgacgtgcg gctctttcag cttgaatttg taggtgccgt gcttcttgaa ttcgtcccac
     8221 gacatggtca caccctggtg gtgctgcacg ttgttggtcc accaggccag cagataggcc
     8281 tcgtccaccg cgtcggggtt ctggaagtag ctgcggtcgg cgcgcgggtt gtagcgcttg
     8341 cccaggttct tcaggttcgg atcgagttct tcgagccggt agctgagttc ggtgaagacc
     8401 tggaagtcgg tcttgctttc gcccagcggc tcgatcacct tgggccggtg gatgtagtag
     8461 tggcccttgt accagggcaa ggcgacgtcg tgccgctcga agtgggtggc aatcggcagc
     8521 aggtaatcgg cccagatgcc cgatggggtg atggtcgaat ccatgcacac caccagttcc
     8581 agcttttcga tcgccttgat ttccttgttg atgttggtga gctggttgaa ccagtcgctg
     8641 ccctgccaga agatggcctt gatgttgggc accttgccgt cgaggtcgtc ctcgcgcggc
     8701 caaaggccca gttcctcccg tttgatgtcg gggtagttca gcacgcagtg cgcccagcgg
     8761 tcggacttga tcgaggccca ccacaggttg gcgttttcgt cgtaggggta ggccacgctc
     8821 tcgctgtgcc aggccttgcc cacgccctcg gcgcaaccgc ccagcacgcc gatgttgccg
     8881 gtcatggcct gcagcgcggc ggccatgcgg ttgtattgct cgccatagct ggcgcgaccg
     8941 ggcgcccaac tggctttcaa tgccgccggt ttggtcttgg cgtacatctc ggccagcttg
     9001 atgatgtcct cggccttcac gccgcagatg gcggcggccc attcgggcgt cttgggcgtg
     9061 ttgtcgtact tgcccaggat gtagtccttg aagttctcct tgcccttgta gtcgccggtg
     9121 gtggtgcccg catccatccc caggcagaac ttgttgatga acttctggtc ttgcaggttg
     9181 ttggtgaaga tgtggtgggc catgccggcc atcatggcgg cgtcggtgtt gggcttgatc
     9241 ggaatccacc acgcgtcgta cacggcggcc gaatcggtgt actgcggatc gatgagcacg
     9301 aacttgcagc ccttctgctt ggccatgcgc atgtaataga aggtgttgcc gccgtggaag
     9361 gtgtaggccg ggttccagcc ccacatgatg atcagcttgc tgtgggcgaa ggtgtcgtcc
     9421 tcgttgccat cctggatggt gccgaaggtc atgcggctgg agaacgtggt gccctggtag
     9481 ctgggcacgc tccacgagtt ggtgcggcag ccgaacatgc ccagaaagcg gcccagcagc
     9541 ccttcgatct ggtcggactt gtgcagcacg ccgtagctgg cgccagcgta gctctggtcc
     9601 aggatggcgg tggggccaaa gtctttcttc agctgcacca tcttttgcgc gatttcgttc
     9661 agcgcctcgt cccagctgat gcgcttgaac ttgtgctcgc cgcgcgcgcc gacgcgcttc
     9721 atggggtaga gcaggcgctc gggcgaatac aggcgcgcgc ggtagctgcg cccgcgcagg
     9781 caggcgcgca gctgcggctc ttcaaacgtg tctttgccgt aggcaccttg agcctggtat
     9841 tcaccgttgt cggtcgacag gcgcacgatc acgtcgccct tcttgtgcgc caccagcacg
     9901 tggcgcgagc cgcagttgtg cgcgcacgag gtgaccacgg tggtcagttg gtcgtcggtg
     9961 aaatagggct cgtaggcaaa ggcctgggcc ttttcgggtg cgatgcccac gccgacgctg
    10021 accagtcccg ccgcgccggc cgtggcgccg acgatcttta ggaagtcgcg ccgcttgtag
    10081 ccggtggaat gctggtcgat ggaatcgttg acgctgggaa agcgcgcggc cgctggcgca
    10141 tccaggactt caggcaggtc gtgcttcatt tcttatctcc ggtctgcgcg acggcggcgc
    10201 tgatgaagcg cacttcactc tcggtggggc gccccttggc ccagtcgggc acgtcggcgg
    10261 ccatgccggg ccaggcgtgg cgcggatagg ggtagtgcgt gcggtaccac gagcggccgc
    10321 cgtggcagcc aaagcacagg tcgccgccat tgggcttggc gcccagttcc tcaatggcct
    10381 tggcgttgag gtaattgacc tggtgccgcg tggtgcggat gccggcgtgg cacagcaggc
    10441 agttgttctg gaacgtggcc ttggactgct cccacgggcc gggcaggccc atgacttcga
    10501 cattcatctt gccgtggcac ttcaggcaca cggtctcggg gttgaccatc ttgcgcagga
    10561 tctgctggcc gctgtcgaag gtggacgagc cgggcgcttc ctcgcgcggg tcgttgcctt
    10621 cgtggcaggt ggtgcagcgc atgttcatca cctgcttggc cagcggcgtt tccaggtggc
    10681 ggcggtgaaa ggtgtcctgt tcgccctggt aggtcgaggt gacctggtac caggccttgg
    10741 cggtgctggc cggcagcccc gctggcgagg tcttgcgcac ggagggcttg agcacttcgg
    10801 cgtggcaggc caggcattgc tggtcggtgg ccttctcgat cgccggtttg aagtggatgg
    10861 ggtcgtactt ggccgcgtgg tagctgaccg gcttgacggc cgctggtgcg ggtgccgcgg
    10921 caggcggtgc gctggcgaca ggcgcgggcg tgatcggctt ggcgttgtca ctgcatcccg
    10981 aggcgagcca cgccgccaac aggcaggaca acccagccag aaccagcctg aaggacaaag
    11041 gccgcatggt gcttgctccg acgagaaaag actttgggaa aaaggctttg agaaacaccg
    11101 ccgcggcagc gagggcgccg cagtcggtcg ccgggccggc atgatggccg gcccggggct
    11161 cacggcagga cagtgcttac ttgctgccgc tcatgcccga catgccttgt tggccgctca
    11221 tgccggacat gccttgctga ccgctcatgc cggacatgcc ttgttggccg ctcatgccag
    11281 acatgccttg ctgaccactc atcccagaca taccggacat gccgctcata ccggacatgc
    11341 cgctcatccc ggacatgcca ctcatgcccg acatgccttg ttggcctgcc ttgcggggct
    11401 ttttcttctt cttggcggcc ttggccttgt tgcccgacat gccgctcatg ccggacatgc
    11461 ctttctggcc gctcatcccg gacatgccgc tcataccttt ctggccgctc atgccggaca
    11521 tgcccgacat gccgctcatg ccctgctggc cactcatgcc ggacatgccn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gccttgctga ccactcatgc ccgacatacc
    11701 ggacatgcct tgctggccag acataccgga catgccttgc tgaccactca tgcccgacat
    11761 accggacatg ccttgctggc cagacatgcc ggacataccg ctcatgccag ggttctgctc
    11821 tccagcggcc ggcttgttgc cgctcatgcc cgacataccg ctcatgccgg attgctgtgc
    11881 catggcgggc agcgctgcca gtgcagcagc catcaccgac aggccaagtg ctttacgaac
    11941 caatttcatg gagatctcct tcattacaag acaaggaaaa aaagggcttt aacgccccct
    12001 gcctgtcggc agcatcgacg caccgccttg cacggccgcg gcgattcggt gagcccagcc
    12061 gcagcagagc cgacggctgg gcgtgatcaa tgcatgggtc atcgcgtgca caaaggtgct
    12121 cagcgggatg cggcgctggc cggtgcgacg ggcgataccg cgctggcgcc ctcatgccag
    12181 ccacgcgggt cgtagggcgg cgtcatgccg gggccgcgca ggggcaccca ccagcgcccg
    12241 gtcgcggcga tggtgtccac gttgccgggg tggggcgccg acacgccgcg cagccagcgg
    12301 gccatttgct cgggtgatgc cggctggtcg gtctcggtcg gggcgcccgc cgggcgctgg
    12361 tggggttgca ggccggcgat gggggcgccc gcaaccggcg tcgcgaccgc tggcgcggag
    12421 gcgcccggcg cctgcgcctg ggcggcggtc aagacagcca gcaggccggc ggtaacgaca
    12481 agaatcggct tcatatcccc atccacatgt gcggtgtgtc tgcagggtat gaagcaaggt
    12541 gcatgccagc ccggtaggct gttgcaagcg cttgtcgcca ttcaatttgc gcgggcagcg
    12601 gtcatctgat ctgccacatt ggcaggacgc cctcgcgccg acctgtcaat ctgtcatgcc
    12661 aatctggcaa agctgccaag cgggccgatt tcaagcttta gccctgatag ggcatgcgtg
    12721 ggcagcgatg aatggacggg ccagcgctca gcgccggaaa cgctgccggg tgaacgccag
    12781 cgcgatccag aaactggcga tggcgtaggc tgccagcacg gccaggtggc gcgcggcatc
    12841 ggccggccac tggtcgagga acagcgggcg caccagttcc accgccgccg tcagcggcag
    12901 ccagtcggtg atggtctgcg cgaagccggg cagctgcgag cgcgggaaga acacgcccga
    12961 gaggaacatc atgggcgaca gcagcaccgc gaagtagtag gtgaagaagt cgtagccctg
    13021 ggccttggcg ttgaacacca gcgccatgct ggcaaaggtg acgcccgcca ccaacccgat
    13081 cggcagcacc agcaccagct tgggagaaag gctgatcttc agcgccagca tcacgcccag
    13141 gatggcgacg accgagaaca aggctttgaa gccggcccac agcatctcgg ccagcaccac
    13201 gtcgtcgagc tgcacggggg tgttgaggat gccgtcccag gtcttttgca cgtgcatgcg
    13261 cgagaaggcc gagtacagcg cctcgaagct ggccgcgttc atggcgctca tgcagatgct
    13321 gccgctggcc aggaacacga tgtagggcac ttcctgccca tgcgtgggta cgcgccccac
    13381 catggcgccc aggccgtagc cgaaggccac cagccacagc agcggctcgc cgatgttggc
    13441 cagcaggctg ggcagcgcca gcttgcgcca caccagcagg ttgcgctgga acaccggcca
    13501 ccagcgcatc gacaggcgcg gcggcgccca gacggaggct tcgttgcggt tagccatctt
    13561 gtcgaatctg tcggcccgtc agcttgagga acaagtcctc caggttggcg ggacggtgca
    13621 aggtgcgcaa ttgcgggtgc gcttccagcg cctgcatcaa gggccgcgcg tggcgggtgt
    13681 agaagaacac cgtctcgccg ctgcgctcga cgcgctcggc gtgcgccgcc agtgttgaat
    13741 cggccagcgc cagcgcgccg tggccgtaga cctcgatcac ctccggctcc aggtgctcgg
    13801 cgatcagctc gcgcgggcgc ccctcggcga tcttgcggcc atggtcgagc accagcaggc
    13861 ggtggcacag gcgctcggcc tcgtccatga agtgcgtggt cagcaggatc gacttgccct
    13921 cggccagcag gcgctgcagg cgctcccaca tcaggtggcg cgcctgtgga tcaagcccgg
    13981 tggtgggttc gtccagcagc agcagatcgg ggttgttgac cagtgcgcgc gccagcgaca
    14041 ggcggcgctt catgccgccc gacagctcgg ccggcttggc gtcggccttg tggccgagcg
    14101 cggcgaagtc gagcaactgc ggcacgcgct cggccatctg cgaccgcgtc agaccgaagt
    14161 agcggccgaa gacgaacaga ttttcggcgc agctgaaatc ggggtcga
//
LOCUS       DAOQTZ010000073        14100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_4882_length_14100_cov_14.983909, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000073 DAOQTZ010000000
VERSION     DAOQTZ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4882_length_14100_cov_14.983909"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1189)
                     /locus_tag="PKN51_09395"
     CDS             complement(44..1189)
                     /locus_tag="PKN51_09395"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNT85352.1"
                     /translation="MSLFVTPQSMHFEAPLPLQSGASIGGYDLRYETYGQLNAERSNA
                     VLICHALNASHHVAGVYEGQERSEGWWDNMIGPGKPVDTDRFFVIGINNLGSCFGSTG
                     PMHVNPATGRAWGADFPVVTVEDWVNAQARLLDRLGIGSLAAVMGGSLGGMQALSWTL
                     QYPERVRHAVVVASAPNLSAENIAFNEVARRAIVTDPDFHGGHFYAQGVLPRRGLRIA
                     RMIGHITYLSDDVMNEKFGRELRQAVAQHASGYRFSTQDVEFQIESYLRYQGDKFSEY
                     FDANTYLLITRALDYFDPARAVGGDLTAALAHTRARFLLVSFSTDWRFSPARSREIVR
                     ALLENRRDVSYAEIDAPHGHDAFLLDDPRYMGVVRSYFARIAQELKA"
     gene            complement(1321..1572)
                     /locus_tag="PKN51_09400"
     CDS             complement(1321..1572)
                     /locus_tag="PKN51_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HNT85353.1"
                     /translation="MAKKFPLRPAHPERICWGCDKYCPAGAMACGNGSDRTPHPAELF
                     GDDWHTWQPPVEGGAAEMASPDVDDPSRDAPVAGGAGPR"
     gene            complement(1594..2163)
                     /locus_tag="PKN51_09405"
     CDS             complement(1594..2163)
                     /locus_tag="PKN51_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNT85354.1"
                     /translation="METFLVATGIVALAEMGDKTQLLALLLAARYRRPWPIVWGIFIA
                     TVVNHALAGALGHAVTRVLGPELLRWVLGASFIAMAVWMLMPDRLDDDGTPAAAPRLG
                     VFGTTVLAFFLAEMGDKTQIATVALAARHPHWLAVVGGTTLGMMLANAPVVWFGDRLL
                     RRLPVRAVHGVSALIFLALGVLAWLGWDN"
     gene            2550..3104
                     /locus_tag="PKN51_09410"
     CDS             2550..3104
                     /locus_tag="PKN51_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNT85355.1"
                     /translation="MGNKLYVGNLPYGVRDADLEQAFAPFGSVTSAKVMMERDTGRSK
                     GFGFVEMGSDAEAQAAINGMNGQSLGGRNVVVNEARPMEARPPRSGGYGGGGYGGGGG
                     GGYGGGRREGGGGYGGGRREGGGYGGGDSGGGYGGRREGGYGGGQDGNFRSPYGSGPR
                     GGRREGGYGGGRQDGGYGGGRDDE"
     gene            complement(3353..4144)
                     /locus_tag="PKN51_09415"
     CDS             complement(3353..4144)
                     /locus_tag="PKN51_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT85356.1"
                     /translation="MARLIFITGASSGIGQALAERCHAEGWHLALVARRADDLRAWAQ
                     ARGWSAERWRVYPADVADIDSIVAAGQACLAQQGVPDVVVANAGISVGMDTAERSDLA
                     VMARTLAVNNVGMAATFQPFVAAMRQRGSGTLVGIASVAAIRGLPGHGAYCASKAGVV
                     AYCESLRGELRGSGVRVVTLLPGYVDTPLTRKNRYPMPFLLSADRFAQRALRAIEQGV
                     SYRVIPWQMALVAKLLRVLPNAWFDRLLSGRPRKARAGQGSATGS"
     gene            complement(4164..4781)
                     /gene="lptC"
                     /locus_tag="PKN51_09420"
     CDS             complement(4164..4781)
                     /gene="lptC"
                     /locus_tag="PKN51_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNT85357.1"
                     /translation="MNRARLWRHGREQLAAWLPVLVMMLFALGSWWLVRSAPRIGPAD
                     GGPPPSAEPDYFMREFSVRSFDADGRLTSELVGVEGQHHPLTDTLAVQQPRIRSFDEQ
                     GNPTLASAQRALAKGDGSEAELFGKARVVREAVTSSDGRVVPRMEFLGEYLHAFIDEH
                     RVSSNQPVELRRGADVFTGNEFAYDDRRGVADLSGRVRGVLQPRR"
     gene            complement(4778..5350)
                     /locus_tag="PKN51_09425"
     CDS             complement(4778..5350)
                     /locus_tag="PKN51_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNT85358.1"
                     /translation="MEPTPAAPALTWPPTLLLRAQAVRVAFFDVDGVLTDGGLYFSAE
                     GETLKRFHTLDGHGIKLLARAGITPCVISGRDSAPLRVRLQALGVTHVRLGTEDKLPA
                     ASDLLAELGLDWSQAAAIGDDWPDLPLLRRATLACAPPGAHAEARACAHHVTVARGGE
                     GAAREFCDLLLVAVGHYRRLLDAQHRGGAT"
     gene            complement(5350..6327)
                     /locus_tag="PKN51_09430"
     CDS             complement(5350..6327)
                     /locus_tag="PKN51_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNT85359.1"
                     /translation="MHFDPAQALRLARETLDIEAAAVVGLKARLGDGFVRAVALMLAA
                     RGRVVVMGMGKSGHVGRKIAATLASTGTPAMFVHPAEASHGDLGMIQPQDVVLAISNS
                     GESDELVSILPLVKRLGAPLVAITGRADSTLARHADAVLDGSVEKEACPLNLAPTAST
                     TAQLALGDALAVALLDARGFRAEDFARSHPGGALGRRLLTRVADVMRAGDEVPRVGPE
                     ASFSALMREITHKGLGASAIVDADGRPVGIFTDGDLRRLVETGRDLRALTAREVMHPR
                     PRHIRDDALAAEAAEAMETHRITVLLVLDAGGRLVGAVNSNDLMRAKVI"
     gene            6750..8735
                     /locus_tag="PKN51_09435"
     CDS             6750..8735
                     /locus_tag="PKN51_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNT85360.1"
                     /translation="MEPLELTLLYLAAAVLGVAACRWARLPAILGYLVVGVLIEPHTL
                     GWAVGQDAEGIKHLAEYGVVFLMFVIGLEFNLPKLHAMRQHVFGLGLAQVLGTMAVVT
                     GGALLLAWLLPQHWPMSWQGALALGGALAMSSTAIVVKMMSDRLELESEHGQRVIGVL
                     LLQDLAVVPLLVLIPALGSSPENLLAELTVAGLKAVGLITILLWGGQRVMRWWLTLVA
                     RRRSQELFVLNLLLMTLGLAWLTEQAGLSLGLGAFICGMLIAETEFKYQVEAEIRPFH
                     DVLLGLFFITIGMLLDWRLVLLQWPVVLILLALILLLKLAVVTGMAALLGASAGTALR
                     TGLYLAQAGEFGFVLLTLSNRQGLLAPELFNPILAAMVVSMLATPFIILGSNRIVLRL
                     VASEWMLQSLHVTRIARQSMDAAGHVLICGYGRSGQAMARLLRQQKIPFIALDIDPDR
                     VRLAAAAGDSVVFGDAATTAALVAAGLGRASTVALTYLDPPSVLKALAAIRAHAPKVP
                     VIVRTQTDRDLDKLRAAGATEVVPEGLEGALILAGHALALSGTPVRRVARLVQEQREQ
                     RYGMLRGFFPGATDDASADPMEEMRLGVFTVPASMAGQALGDMLVQLGELRAISLRLP
                     TGTPATPENEIVLQGGEVLLLSGRPEALARAEQALAT"
     gene            8756..9313
                     /locus_tag="PKN51_09440"
     CDS             8756..9313
                     /locus_tag="PKN51_09440"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNT85361.1"
                     /translation="MSTAFFVSHYLRQHIRTVPDWPAPGVQFRDITPLLQNPQVFRVL
                     IDAFVHRYMDPALRPDVVAGLDARGFILGSVVAYELNVGFVPIRKKGKLPFTTVEETY
                     ELEYGSATVELHTDAVQRGQRVLLIDDLIATGGTMLAGWRLLQKLGAHVTEGAAIVEL
                     PELGGSARLTAAGLPLFTLVTFDGH"
     gene            9400..10050
                     /locus_tag="PKN51_09445"
     CDS             9400..10050
                     /locus_tag="PKN51_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT85362.1"
                     /translation="MNPPSVQDPAPHAPRRGRPRKSAQERDESQRRAELLRAAARLFR
                     HQGFDATSTRDIAAAAGMQSGSPFYYFESKSALLYAVMHDGMAMVTQAQAQALQALGP
                     RPTARERLRALIRHHFEVLVGPASDFIPVMLYEWRALTPEQRARIGALKDRYEAAWMP
                     VLGSLRRSGSLRARPEVARLFIFGALNWTVQWFDPRGALSLDELTEQALRLFLGDR"
     gene            10051..10944
                     /locus_tag="PKN51_09450"
     CDS             10051..10944
                     /locus_tag="PKN51_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT85363.1"
                     /translation="MYQSVFAPRLLTGQTIIVTGGGSGIGRCSAHELAALGAHVVLVG
                     RNADKLRATADEITQDGGAVCWLACDIRQEGAVQETVRAVVAGRGRIDGLVNNAGGQY
                     MTPLEKISAKGWDAVVATNLTGGFLVARECFAQSMRQHGGAIVNIVADMWGSMPGMGH
                     SGAARAGMVSFTETAAVEWAAHGVRVNAVAPGYIASSGMDHYPPEAGAMLRAMRQTVP
                     LGRFGTEAEVSAAIAFLLSPAASFISGSVLRVDGARPQVRMGWPMGVPGASVRQRDAV
                     KPFNGFHRAVTPKVFAEPESP"
     gene            10941..12584
                     /locus_tag="PKN51_09455"
     CDS             10941..12584
                     /locus_tag="PKN51_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNT85364.1"
                     /translation="MSLFESSWNAASDTAQARRARMLARIEAWRALEQRSADKSGEAA
                     ARFAKRGQLLPRERVALLLDRGAPWLPLAALAGYLRDVPEPDQSVPGGGMIAGIGFVA
                     GTRCMVVASDSGIDAGAVQPGGLDKMLRVQEIALRERLPFIHLVESAGADLMRYRVEG
                     FVLGGGLFRNLARLSAAGIPVITVQHGSGTAGGAYMPGLSDIVIMVEGRSRAFLAGPP
                     LLKAATGEVATEEELGGALMHTTVSGLGEYLARDDREALGLARRVIGDLQWSNRASAL
                     SGQAPTAIKSEVIEPPWSADELLTLMPAHHREPVDMREVAVRLVDGGALHEFKAGYGS
                     ATVCAQGHIGGHAVGFITNNGPIDNQGANKATHFIQWMCQLGHPIIYLQNTTGYMVGK
                     DVEQAGMIKHGSKMIQAVTNATVPQLTIQCGASFGAGNYGMCGRGFAPRFLFSWPGAA
                     TAVMGGEQAAGTMRIVTEAALKRKGIEPDAAKMQKQFDAVVAMFERQQDAFTTSGLLL
                     DDGVIDPRDTRAVLRFCLDTLAEGAAHTPRPMQFGVARM"
     gene            12607..13785
                     /locus_tag="PKN51_09460"
     CDS             12607..13785
                     /locus_tag="PKN51_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT85365.1"
                     /translation="MQLTHEHEQIRDTLKRYIAEHINPHVDEWEEAEIFPAHQVFKGL
                     GDLGLLGLTKPVEYGGMGLDYSYSMVMAETLGLINCGGVPMAIGVQTDMCTPALARFG
                     SDELKRQYLAPAIAGDMVGCIGVSEPGAGSDVSGIKSVARKDGDDYVITGQKMWITNS
                     LQADWMCMLVNTGGDNPHKNKSLILVPMREGPGGPLRKGISVARKIRKIGMHSSDTGL
                     IHFDEVRVPQRNLIGAEGQGFIYQMQQFQEERLWAAANALSALDGCIRDTIEWARERK
                     MFGATLLDQQWVQFKLVELQTEVEALRALTWKAGERYVAGDDVLQWASMGKLKAGRLM
                     REVADTCLQFWGGMGFASDSRVSRLYRDGRLGSIGGGADEVMMGIIAKTMGMAKRPGA
                     "
     gene            13787..>14100
                     /locus_tag="PKN51_09465"
     CDS             13787..>14100
                     /locus_tag="PKN51_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNT85366.1"
                     /translation="MKRILIANRGEIARRVIATARRMGLETVAVHSDADANALHVREA
                     TLACALGGNSSADSYLRVDKLLAAARATGADAVHPGYGFLSEDAGFAQAVQDAGLTWI
                     GPP"
BASE COUNT         2206 a         4819 c         4942 g         2133 t
ORIGIN      
        1 ctgacgcgct gcgggattcg cgcttgggag cggcccggcg cgctcatgcc ttcagctcct
       61 gcgcgatgcg cgcgaagtac gagcgcacca cgcccatgta gcgcggatcg tccagcagga
      121 aggcgtcgtg cccgtgcggg gcgtcgatct cggcgtagct gacgtcgcgc cggttttcca
      181 gcagcgcgcg cacgatctcg cgcgagcgcg caggcgaaaa acgccagtcg gtgctgaagc
      241 tgaccagcag aaagcgcgcc ctcgtgtgcg ccagcgccgc cgtcaggtcg ccgccgacgg
      301 cgcgcgccgg gtcgaagtag tcgagcgcgc gggtgatcag caggtaggtg ttggcgtcga
      361 agtattcgct gaacttgtcg ccctggtagc gcaggtagct ctcgatctgg aactccacgt
      421 cctgcgtgct gaagcggtag ccgctggcgt gctgggccac ggcctgacgc agttcgcggc
      481 cgaacttctc gttcatcacg tcgtcgctca agtaggtgat gtggccgatc atgcgggcga
      541 tgcgcaggcc gcgccgcggc agcacaccct gagcatagaa gtggccgcca tggaagtccg
      601 ggtcggtcac gatggcgcgc cgcgccacct cgttgaaggc gatgttctcg gccgacaggt
      661 tgggcgcgct ggccaccacc acggcgtggc gcacgcgctc ggggtattgc agcgtccagc
      721 tgagcgcctg catgccgccc aggctgcccc ccatgacggc ggccagtgag ccgatgccca
      781 ggcgatcgag cagacgcgcc tgcgcgttga cccagtcttc caccgtcacc accggaaagt
      841 cggcacccca ggcgcggccc gtggcggggt tgacgtgcat cggcccggtg gagccgaagc
      901 acgagcccag gttgttgatg ccgatgacga agaaacggtc ggtgtcgacc ggcttgccgg
      961 ggccgatcat gttgtcccac cagccctcgc tgcgttcctg cccttcgtac accccggcca
     1021 catggtgcga ggcgttgagc gcgtggcaga tcagcacggc gttgctgcgc tcggcgttga
     1081 gctggccgta ggtttcgtag cggaggtcgt agccgccgat cgaggcgccg ctctgcagtg
     1141 gcagaggcgc ctcgaaatgc atggactgcg gcgtgacgaa caaggacatg gcaacaaaaa
     1201 acccggcttc gccaaatcga aaccgggttg catgcgtgtc tcggatttag cggtatttgt
     1261 agagcgcccg caagcagagg caaatcggcg ctggatcggg ccaaagtata gcggcaggcg
     1321 tcagcgcggg ccagcgccgc ccgcaacggg cgcgtcgcgg ctggggtcgt cgacgtcggg
     1381 cgacgccatc tcggccgcgc caccttcgac cggcggctgc catgtgtgcc agtcgtcgcc
     1441 gaacagctcg gccgggtggg gcgtgcggtc ggagccgttg ccacaggcca tggcgccggc
     1501 cggacagtac ttgtcacaac cccagcagat gcgctcgggg tgcgccggcc ggagggggaa
     1561 cttcttcgcc atggcggtgc ggtgcaagcg gcctcaattg tcccacccca gccatgccag
     1621 cacacccagt gcaagaaaga tcagcgccga caccccatgc acggcgcgca ccggcagccg
     1681 gcgcagcagc cggtcgccga accacaccac cggcgcgttg gccagcatca tgcccagagt
     1741 ggtgccgccc accacagcca gccagtgcgg gtgccgggcc gccagggcga cggtggcaat
     1801 ctgcgtcttg tcgcccattt ccgcgaggaa gaacgccagc acggtggtgc cgaacacccc
     1861 caagcgcggg gcggcggccg gcgtgccgtc atcgtccagc cgatcgggca tcagcatcca
     1921 caccgccatg gcgatgaagg atgcccccag tacccagcgc agcagttccg gccccagcac
     1981 gcgggtgacc gcatggccga gcgcgccggc cagcgcatga ttgaccacgg tggcgatgaa
     2041 aatgccccac acgatcggcc agggccggcg atagcgcgcc gccagcagca gggccagcaa
     2101 ctgcgtcttg tctcccattt cggccagcgc gacgattccg gtggcaacga gaaaggtttc
     2161 catggggaag ggcgtggcga atacagacaa aatcgccatc gggcttgaca ggtctgcgcc
     2221 agaagctatt cattcaatag caaaaaacta ccgtgcagcg acggcgcgcg accggaacat
     2281 ggcggccgat tgtaggcgcg ggcccaccgc gccgaccggg aaccggcccc cgtcgcaaat
     2341 acctgagttt ttttatcaac caaaaatcgc gcgcttgact ttcgcagttc tgacgcataa
     2401 tcgcgcgggt gtcccagcgg ttggggcatt ccacgtggct tttgcggtga tttcacagtc
     2461 tcagttgccc ttcttggccg tgccaaggtt cggtgaaggc gtttcgcgga ctccatcgct
     2521 tttcatttgt ttttgaggag tccctttcaa tgggcaacaa actttacgtc ggcaacctgc
     2581 cgtatggcgt gcgtgacgcc gatctggagc aggccttcgc acctttcggc tcggtcacca
     2641 gcgccaaggt gatgatggag cgcgacaccg gccgctccaa gggcttcggg ttcgtcgaga
     2701 tgggcagtga cgccgaggcg caggccgcca tcaacggcat gaacggccag tcgctgggtg
     2761 gccgcaacgt ggtcgtcaat gaagcgcgcc cgatggaagc gcgtccgccc cgttccggtg
     2821 gctacggcgg cggcggctat ggcggcggcg gtggcggtgg gtacggcggc ggccggcgtg
     2881 aaggcggcgg cggctacggc ggcggccggc gtgaaggcgg tggctacggc ggcggcgact
     2941 ccggtggcgg ctacggcggc cgacgcgaag gcggttacgg cggcggccag gacggcaact
     3001 tccgcagccc ctacggctcc ggtccgcgcg gcgggcggcg tgagggtggt tacggcggtg
     3061 gccgccagga cggcggctat ggcggcggcc gcgacgacga ataagcatcg tccccgcgct
     3121 gctttcgcgc catcgatacc aaaggctccg caaggagcct tttttcatct tggctgcggc
     3181 ccttgaacac acccgtccgt aacgtggtcg gtgtggccga cacaacgcca cggtgcggcg
     3241 cgcgcgggtg cgccgcgtgc cgacccagca aacgaaggtc agcgacgctg cgtgaccctg
     3301 gagggattca aaccgcccgc agtcggccag cggttcacgg ccgttgcagg gctcatgacc
     3361 ccgtggccga accctggccg gcgcgcgcct tgcggggccg gccgctcagc agacggtcga
     3421 accacgcgtt cggcaacacg cgcaacagct tggcgaccag cgccatctgc cacggaatca
     3481 cgcgatagct cacgccctgt tcgatggcac gcaacgcccg ctgtgcaaaa cggtcggccg
     3541 acagcagaaa cggcatcgga tagcggttct tgcgcgtcag cggcgtatcg acgtagcccg
     3601 gcagcagggt gacgaccctg acgccgctgc cgcgcagctc gccgcgcagg ctttcgcaat
     3661 acgccaccac gccggccttg ctggcgcagt acgcgccatg gccgggcagg ccacggatcg
     3721 cggccacgct ggcgatgccc accagggtgc cgctgccgcg ctggcgcatc gccgccacga
     3781 aaggctggaa cgtcgccgcc atgccgacgt tgttgaccgc cagcgtgcgc gccatgacgg
     3841 cgaggtcgct gcgctcggcg gtatccatgc cgacgctgat gccggcgttg gccaccacca
     3901 cgtcgggcac accctgctgc gccagacagg cctggccggc ggcgacgatg ctgtcgatgt
     3961 cggccacgtc ggccgggtag acccgccagc gctcggccga ccagccacga gcctgcgccc
     4021 aggctcgcag atcgtcggca cggcgcgcca ccagggccag atgccagcct tcggcatggc
     4081 agcgttcggc cagcgcctgc ccgatgccgc tggaggcgcc ggtgatgaag atcagcctgg
     4141 ccatcgtggt gcgggcgatc ggctcagcgc cgcggttgca gcacgccgcg cacccgaccg
     4201 ctcagatcgg cgacaccgcg gcgatcgtcg taggcgaact cgttgccggt gaacacgtcg
     4261 gcaccgcgcc gcagttcgac cggctggttc gagctgaccc gatgctcatc gatgaaggcg
     4321 tgcaggtatt cacccaggaa ttccatgcgc ggcaccacgc ggccgtcgga tgacgtgacg
     4381 gcctcgcgca ccacgcgcgc cttgccgaac agttcggctt cggagccgtc gcccttggcc
     4441 agcgcgcgct gggccgaagc cagcgtgggg ttgccctgct cgtcgaacga acggatgcgc
     4501 ggctgctgca ccgccagcgt gtcggtcagc ggatggtgct gaccttcgac gccgacgagc
     4561 tcggatgtca ggcgtccatc ggcatcgaag ctgcgcacgg aaaactcacg catgaagtag
     4621 tccggctcgg ccgacggtgg cggcccgccg tccgccgggc cgatgcgcgg ggcgctgcgc
     4681 accagccacc agctgccgag cgcgaacagc atcatcacca gcaccggcag ccaggccgcc
     4741 agttgttcgc gcccatggcg ccacagtcgc gccctgttca tgtcgcgcca ccccggtgct
     4801 gggcgtccag caggcggcgg tagtgcccca cggccaccag cagcaaatcg cagaactcgc
     4861 gcgcggcacc ttcgccacca cgcgccaccg tcacgtggtg cgcacaggcc cgggcctcgg
     4921 catgggcgcc cggcggcgcg caggccaggg tcgcgcggcg cagcaacggc aggtcgggcc
     4981 agtcgtcgcc gatggccgcg gcctgcgacc agtccagccc cagctcggcc agcaggtcgc
     5041 tggccgccgg cagcttgtct tcggtgccca ggcgcacatg ggtcacgccc agcgcctgca
     5101 gacgcacacg cagcggcgcc gagtcgcgcc ccgaaatgac gcagggcgtg atgccggcgc
     5161 gggccagcag cttgatgccg tggccgtcca gggtgtgaaa gcgcttgagc gtctcgccct
     5221 cggcgctgaa gtacaggcca ccatcggtca gcacaccgtc gacgtcgaag aaggccacgc
     5281 gcactgcctg cgcgcgcagc agcagggtcg gcggccaggt cagtgcaggc gctgcgggcg
     5341 tgggctccat cagatcacct tggcgcgcat caggtcgttg ctgttgaccg cgccgaccag
     5401 ccgcccaccc gcatccagca ccagcagcac cgtgatgcgg tgcgtttcca tggcctcggc
     5461 ggcttcggcg gccagcgcat cgtcgcggat gtggcgcggc cgcggatgca tcacctcgcg
     5521 ggcggtgagc gcgcgcagat cgcgcccggt ctcgaccagg cggcgcaggt caccgtcggt
     5581 gaagatgccc accggccggc cgtcggcgtc gacgatggcc gaggcgccca gccccttgtg
     5641 cgtgatttcg cgcatcagtg cgctgaaact ggcctccgga ccgacccgcg gcacctcgtc
     5701 gccagcgcgc atgacatcgg ccacccgcgt gagcagtcgt cggcccaggg cgccgcccgg
     5761 gtgcgagcgg gcaaagtcct cggcccggaa gccgcgcgcg tcgagcaggg ccaccgccag
     5821 tgcgtcgccc agcgccagct gcgccgtggt gctggcggtg ggcgccaggt tgagcgggca
     5881 ggcttccttt tcgacgctgc cgtcgagcac ggcgtcggca tggcgcgcca gtgtggagtc
     5941 ggcgcggccg gtgatcgcca ccagcggcgc ccccagccgc ttgaccagcg gcaggatgga
     6001 gaccagctcg tcgctttcgc cactgttgga gatggccagc accacgtcct gtggctggat
     6061 catgcccaga tcgccatggc tggcctcggc cgggtgcacg aacatggccg gggtacccgt
     6121 ggacgccagc gtggccgcga tcttgcgacc gacatggccg ctcttgccca tgcccatcac
     6181 caccacccgc ccacgcgccg ccagcatcaa cgccacggca cggacaaagc cgtcgcccag
     6241 ccgcgccttg aggccgacca cggccgcggc ctcgatgtcc agcgtctcac gggccagacg
     6301 cagcgcctgg gcagggtcga agtgcatcgc aggattctag tgtggtgcgc tgccatggct
     6361 tgtgctttca gagccccaga aggcgtccag acaaggcgca gcccgaagcc catggctgat
     6421 cccttggcca gggctgcaac gcggcatggg cgccttccgg gggctccctt tgggcgatcc
     6481 gctgagcgac tgcctgtggc gttgcgcccg cttgcatcga gccgctcagc gactcgctga
     6541 aagtgccagt ccactgaaac attgaaatgg gcggcgaggg acacccgaga cgggaggcga
     6601 tgcaaggcgc aaagcgcagc cacagcccgt gctgtggcga gcattggcaa cgccgcagcg
     6661 tgcccgcatc gggcggacgg cgccgatccg atttcagtgt ttcagtggac ccgccatggc
     6721 ggcgcaccac accacggtag catgcgggca tggaacccct ggagctgacg ctgctgtacc
     6781 tggccgccgc cgtgctgggc gtggcggcct gccgctgggc gcgcctgccg gccatcctgg
     6841 gctacctggt ggtgggtgtg ctgatcgagc cgcacacgct gggctgggcg gtcggtcagg
     6901 atgccgaggg catcaagcac ctggccgaat acggcgtggt gttcctgatg ttcgtgatcg
     6961 ggctggagtt caacctgccc aagctgcacg ccatgcgcca gcacgtgttc ggcctgggtc
     7021 tggcgcaggt gctgggaacc atggccgtgg tgaccggcgg cgccctgttg ctggcctggc
     7081 tgctgccgca gcactggccg atgagctggc agggtgcgct ggcgctgggc ggcgcgctgg
     7141 ccatgagcag caccgccatc gtggtcaaga tgatgagcga ccggctggag ctggagtccg
     7201 agcacggcca gcgcgtcatc ggcgtgctgc tgctgcagga cctggcggtg gtgccgctgc
     7261 tggtgctgat ccctgcgctc ggttcgagcc cggagaacct gctggccgag ctgaccgtgg
     7321 ccggactgaa ggcggtgggc ctgatcacca tcttgctgtg gggtggacag cgggtgatgc
     7381 gctggtggct gaccctggtg gcgcgccgcc gcagccagga actgttcgtg ctcaacctgc
     7441 tgctgatgac cctggggctg gcctggctga ccgaacaggc gggcctttcg ctggggctgg
     7501 gcgccttcat ctgcggcatg ctgatcgcag agaccgagtt caagtaccag gtggaggccg
     7561 aaatccgtcc cttccacgac gtgctgctgg gcctgttctt catcaccatc ggcatgttgc
     7621 tggactggcg cctggtgctg ctgcaatggc cggtggtgct cattctgctg gcgctgatcc
     7681 tgctgctgaa gctggccgtg gtgacgggca tggccgccct gctgggcgcc agcgccggca
     7741 cggcgctgcg caccggcctg tacctggcgc aggcgggcga gttcggtttc gtgctgctga
     7801 cgctcagcaa ccgtcaggga ctgctggcgc cggagctgtt caacccgatc ctggccgcca
     7861 tggtggtgtc gatgctggcc acgccgttca tcatcctggg cagcaatcgc atcgtgctgc
     7921 gtctggtggc cagcgaatgg atgctgcagt cgctgcacgt gacccgcatt gcccgccagt
     7981 cgatggacgc cgccggccat gtgctgatct gcggctatgg ccgcagcggc caggccatgg
     8041 cgcgcctgct gcggcagcag aagattccgt tcatcgcgct cgacatcgac cccgaccggg
     8101 tgcgcctggc ggccgccgcc ggcgacagcg tggtgttcgg tgacgccgcc accacggcgg
     8161 cgctggttgc tgccggtctg gggcgtgcca gcacggtggc cctgacctat ctggatccgc
     8221 ccagcgtgct caaggcgctg gcggcgatcc gcgcgcatgc gcccaaggta ccggtgatcg
     8281 tgcgcaccca gaccgaccgc gacctcgaca aactgcgcgc cgccggcgcc accgaggtgg
     8341 tgcccgaagg cctggagggc gcgctgatcc tggccggcca tgcgctggcg ctgtcgggca
     8401 cgccggtgcg gcgtgtggcg cgcctggtgc aggagcagcg cgaacagcgc tacggcatgt
     8461 tgcgcggctt ctttcccggc gccaccgacg acgccagcgc cgacccgatg gaagagatgc
     8521 gcctgggcgt gttcacggta ccggcgtcga tggccggaca ggccctggga gacatgctgg
     8581 tgcagctggg cgagttgcgc gccatcagtc tgcgactgcc cacgggcacg ccggcgacgc
     8641 ccgaaaacga gatcgtcctg caaggcggcg aggtccttct gctgtccggc cgccccgagg
     8701 cgctggcacg agcggagcag gccctggcca cctgatggcg cccgataatg gcgccatgag
     8761 caccgccttt ttcgtctcgc actacctgcg ccagcacatc cgcaccgtgc ccgactggcc
     8821 ggcgccgggc gtgcagtttc gcgacatcac gccactgctg cagaacccgc aggtgtttcg
     8881 cgtgctcatc gacgccttcg tgcaccgcta catggaccct gcgctgcgcc ccgacgtcgt
     8941 cgccggcctg gatgcgcgcg gcttcattct gggcagcgtg gtggcctacg agctcaacgt
     9001 cggcttcgtg ccgattcgca agaagggcaa actgcccttc accacggtcg aggaaaccta
     9061 cgagctcgaa tacggcagcg ccacggtgga gctgcacacc gatgccgtgc aacgcggcca
     9121 gcgcgtgctg ctgatcgacg acctgatcgc caccggcggc accatgctgg ccggctggcg
     9181 cctgctgcag aagctcggcg cccacgtgac cgagggcgcc gccatcgtcg agctgccgga
     9241 actgggaggg tcggctcggc tgaccgccgc cgggttgccc ttgttcacgc tggtgacctt
     9301 cgacgggcac tgaaggcgcc ctggccaccc ttggccacgc accttatttt taccaagcaa
     9361 ccgcttgatt aaataaaagc gctctgctac gcttgaggga tgaaccctcc cagcgtccag
     9421 gacccagcgc cgcacgctcc gcgccgcggg cgcccgcgca agagcgcgca ggagcgcgac
     9481 gaaagccagc gccgtgccga gcttctgcgc gcggcggcgc ggctgtttcg ccatcagggt
     9541 ttcgacgcca ccagcacacg cgacatcgcc gctgctgcgg gcatgcagag cggctcgccg
     9601 ttctactact tcgaaagcaa gagcgcgttg ctgtacgccg tcatgcacga cggcatggcc
     9661 atggtcaccc aggcgcaggc acaggcgctg caggcgctgg ggccgcggcc cacggcgcgc
     9721 gagcggctgc gggcgctgat ccgccaccac ttcgaggtgc tggtggggcc ggccagcgac
     9781 ttcatcccgg tcatgctgta cgagtggcgc gcgctcacgc ccgaacagcg cgcgcgcatc
     9841 ggcgcactga aagaccgcta cgaagccgcc tggatgccgg tgctgggcag cctgcgccgc
     9901 agcggatcgt tgcgggcgcg gccggaggtg gcgcggctgt tcatcttcgg cgcgctcaac
     9961 tggacggtgc agtggttcga tccgcgcggc gcgctgtcgc tcgatgaact gaccgaacag
    10021 gcgctgcgcc tgttccttgg agatcgctag atgtaccaat ccgtgtttgc gccccgcctg
    10081 ctgacggggc agaccatcat cgtcaccggc ggtggctcgg gcatcggccg ctgcagcgcg
    10141 catgagctgg cggcgctggg cgcccacgtg gtgctggtgg ggcgcaacgc cgacaagctg
    10201 cgcgccaccg ccgacgagat cacccaggac ggcggcgccg tgtgctggct ggcctgcgac
    10261 atccgtcagg aaggcgccgt gcaggaaacg gtgcgcgccg tggtggccgg acgcgggcgc
    10321 atcgacggcc tggtcaacaa cgccggcggc caatacatga cgccgctgga aaaaatcagc
    10381 gccaagggct gggacgcggt ggtggccacc aacctgaccg gcggctttct ggtggcgcgc
    10441 gagtgttttg cgcagtcgat gcggcagcac gggggcgcca tcgtcaacat cgtggccgac
    10501 atgtggggct cgatgccggg catgggccac agcggtgcgg cgcgtgccgg catggtcagc
    10561 ttcaccgaaa ccgcggcggt cgagtgggcg gcgcacggcg tgcgcgtcaa cgccgtggcg
    10621 ccgggctaca tagcctccag cggcatggac cactacccgc ccgaggccgg tgccatgctg
    10681 cgcgccatgc gccagacggt gccgctgggg cgcttcggca ccgaggccga ggtgtcggcg
    10741 gcgatcgcct ttctgctcag cccggcggcc agcttcatca gcggcagcgt gttgcgcgtg
    10801 gatggcgcgc gccctcaggt gcgcatgggc tggcccatgg gcgtgccagg cgcatcggtg
    10861 cgccagcgcg acgccgtcaa acccttcaac ggctttcaca gggcggtcac gcccaaggtg
    10921 tttgccgagc cggagtcacc atgagtctgt ttgaatcgag ctggaacgcc gccagcgaca
    10981 ccgcccaggc gcgccgcgcc cgcatgctgg cgcgcatcga agcctggcgt gcgctggagc
    11041 agcgcagcgc cgacaagtcc ggcgaggccg ccgcccgttt tgccaagcgc ggccagctgc
    11101 tgccacgcga gcgcgtggcc ctgctgctcg accgcggcgc gccctggctg ccgctggccg
    11161 cgctggccgg ttatctgcgc gacgtgcccg agcccgatca atcggtgccc ggtggcggca
    11221 tgattgccgg catcggcttc gtggctggca cgcgctgcat ggtggtggcc agcgactcgg
    11281 gcatcgacgc cggggcggtg cagcccggcg ggctggacaa gatgctgcgc gtgcaggaga
    11341 tcgcgctgcg cgagcgcctg cccttcatcc acctggtcga gagcgccggc gccgatctga
    11401 tgcgctaccg ggtcgagggc ttcgtgctgg gtggcgggct gtttcgcaac ctggcgcgcc
    11461 tgtcggccgc tggcattccg gtcatcaccg tccagcacgg ctcgggcacg gcgggcggcg
    11521 cctacatgcc ggggctgtcg gacatcgtga tcatggtcga ggggcgctcg cgcgccttcc
    11581 tcgccggccc gccgctgctc aaggccgcca ccggcgaggt cgccaccgag gaggagctgg
    11641 gcggcgcgct gatgcacacc acggtctctg gcctgggcga atacctggcg cgcgacgacc
    11701 gcgaggcgct cggcctggcg cgccgggtga ttggcgatct tcaatggtca aatcgtgctt
    11761 cagccctttc tggacaagcg ccaacagcta tcaaatcaga agtaattgag cccccctggt
    11821 cggccgacga actgctcacc ctgatgcccg cccaccaccg cgagccggtg gacatgcgcg
    11881 aggtcgcggt gcggctggtc gatggcggcg cgctgcacga gttcaaggcc ggctatggca
    11941 gcgccaccgt gtgcgcgcag gggcacatcg gcgggcatgc ggtgggcttc atcaccaaca
    12001 acggcccgat cgacaaccag ggggccaaca aggccacgca cttcatccag tggatgtgcc
    12061 agctgggcca ccccatcatc tacctgcaga acaccaccgg ctacatggtc ggcaaggacg
    12121 tggagcaggc cggcatgatc aagcacggca gcaagatgat ccaggccgtc accaacgcca
    12181 cggtgccgca gctcaccatc cagtgcggcg ccagctttgg cgccggcaac tacggcatgt
    12241 gcgggcgcgg cttcgcgccg cgcttcctgt tcagctggcc gggcgctgcc accgccgtga
    12301 tgggcggcga gcaggccgcc ggcaccatgc gcatcgtcac cgaagcggcg ttgaagcgca
    12361 aaggcatcga gcccgatgcg gccaagatgc aaaagcagtt cgacgccgtg gtcgccatgt
    12421 tcgagcgcca gcaggacgcc ttcaccacca gcggcctgct gctggacgac ggcgtgatcg
    12481 acccgcgcga cacccgcgcc gtgctccgct tctgtctgga caccctggcc gagggcgccg
    12541 cccacacgcc aaggcccatg cagtttggcg tggcgcggat gtgaatttca ccctcaggag
    12601 acccccatgc aactgaccca cgagcacgaa cagatccgcg acacgctcaa gcgctacatc
    12661 gccgagcaca tcaacccgca cgtcgatgag tgggaagagg ccgagatctt ccccgcccac
    12721 caggtgttca aagggctggg cgatctgggc ctgctgggcc tgaccaaacc ggtggaatac
    12781 ggcggcatgg gcctggacta ttcctacagc atggtgatgg ccgagaccct gggcctgatc
    12841 aactgcggcg gcgtgcccat ggccattggt gtgcagaccg acatgtgcac gccggcgctg
    12901 gcgcgctttg gcagcgacga gctcaagcgc cagtacctcg cgcccgccat cgccggcgac
    12961 atggtgggct gcatcggcgt gagcgagccg ggcgccggca gcgacgtgtc gggcatcaag
    13021 agcgtggcgc gcaaggacgg cgacgattac gtcatcaccg gccagaagat gtggatcacc
    13081 aacagcctgc aggccgactg gatgtgcatg ctggtgaaca ccggcggcga caacccgcac
    13141 aagaacaaga gcctgatcct ggtgcccatg cgcgaggggc cgggcggccc gttgcgcaag
    13201 ggcatctcgg tggcgcgcaa gatccgcaag atcggcatgc acagcagtga caccggcctg
    13261 atccacttcg acgaggtgcg cgtgccgcag cgcaacctga tcggcgccga gggccagggc
    13321 ttcatctacc agatgcagca gttccaggaa gagcgcctgt gggcggcggc caatgcgctg
    13381 tcggcgctcg atggctgcat ccgcgacacc atcgagtggg cgcgcgagcg caagatgttc
    13441 ggcgccacct tgctcgacca gcagtgggtt cagttcaaac tggtcgagtt gcagaccgag
    13501 gtcgaggcgc tgcgcgcgct cacctggaag gccggcgagc gctacgtcgc cggcgacgac
    13561 gtgctgcaat gggccagcat gggcaagctc aaggccgggc ggctgatgcg cgaggtggcc
    13621 gacacctgcc tgcagttctg gggcggcatg ggctttgcca gcgacagccg cgtctcgcgc
    13681 ctgtaccgcg acggccgcct gggttccatc ggcggcggcg ccgacgaggt gatgatgggc
    13741 atcatcgcca agaccatggg catggcgaaa cgcccgggag cgtgacttga aacgcatcct
    13801 catcgccaac cgcggcgaga tcgcgcgccg cgtgatcgcc acggcgcgcc gcatgggcct
    13861 ggagaccgtg gccgtgcatt ccgacgccga cgcgaatgcc ctgcacgtgc gcgaagccac
    13921 gctggcctgt gcgctgggtg gcaacagctc ggccgacagc tacctgcgcg tcgacaagct
    13981 gctggccgcc gcccgcgcca ccggcgccga tgcggtgcac cccggttatg gctttctgag
    14041 cgaggacgcc ggttttgccc aggcggtgca ggacgccggc ctgacctgga tcggcccgcc
//
LOCUS       DAOQTZ010000074        13773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5013_length_13773_cov_13.369004, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000074 DAOQTZ010000000
VERSION     DAOQTZ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5013_length_13773_cov_13.369004"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PKN51_09470"
     CDS             complement(<1..197)
                     /locus_tag="PKN51_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HNT85367.1"
                     /translation="MLSALDHAMMRRALTLAERGRRLSPPNPAVGCVLVDAAATVLGE
                     GFTQPVGQAHAEVMALRDAAAR"
     gene            complement(210..521)
                     /locus_tag="PKN51_09475"
     CDS             complement(210..521)
                     /locus_tag="PKN51_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85368.1"
                     /translation="MPTFTRPAISLQQAVAETPTLAHLSRLATESAARLAVLRPLLPP
                     ALRPLVQAGAPEGDAWTLLAPHQAAAAKLRQLAPALQAALAAAGHPVSTLRVKVAQPL
                     R"
     gene            733..3525
                     /gene="secA"
                     /locus_tag="PKN51_09480"
     CDS             733..3525
                     /gene="secA"
                     /locus_tag="PKN51_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNT85369.1"
                     /translation="MATILTKIFGSRNDRLLKQYRKTAAAINALEPALEKLSDDELRA
                     KTDEFRQRLAGGESLDKLLPEAFAVVREGSKRVMKMRHFDVQLVGAMALHEGKIAEMR
                     TGEGKTLTATLAVYLNALSGKGVHVVTVNDYLAGRDAEWMGRLYNFLGLTVGVNLPQM
                     ARADKQAAYQADITYGTNNEFGFDYLRDNMVYEPGERVQRPLNYAIVDEVDSILIDEA
                     RTPLIISGQAEDHTALYLAINAVVPLLTRQEGEADPRTGEGVTKPGDFTADEKSHQVF
                     LTEQGHETAERVLAERGLIPPGASLYDAANITLMHHLNAALRAHHLFHRDQHYVVQQG
                     EHGPEIVIVDEFTGRLMSGRRWSDGLHQAVEAKEGVPIQAENQTLASITFQNYFRLYR
                     KLAGMTGTADTEAYEFQDIYGLETVVIPPNKPSRRDDQLDRVYKSTQEKYDAAITDIR
                     ECHAKGQPVLVGTTSIENSEIISHLLQKEKLPHQVLNAKQHAREADIVAQAGRPGMIT
                     IATNMAGRGTDIVLGGNVEKAVQALEADASIDEATRQARAAELRAQWQQDHELVKSLG
                     GLRIIATERHESRRIDNQLRGRSGRQGDPGSSRFYLSLDDALMRIFAGDRVRAIMDKL
                     KMPEGEAIEAGIVTRSIESAQRKVESRNFDIRKQLLEYDDVSNDQRKVIYQQRNEILD
                     ASDLSAQIASLREGCFTDLVRQFVPAESVEEQWDIAGLEKALADDWHIELPLRQQIES
                     SSAISDEDILERVLKAADEAYAAKVAQVGAENFGPFERAVLLQAIDTHWREHLSALDY
                     LRQGIHLRGYAQKQPKQEYKREAFELFGQLLDVIKNEVTRVLMTVRIESPEQAGQSAA
                     AMEQRAEHISNVTYTAPTETGEAQTVTEAQARGAAAAAGGEVAAAAAAVPRVGRNDPC
                     PCGSGKKYKQCHGALT"
     gene            3664..4893
                     /gene="argJ"
                     /locus_tag="PKN51_09485"
     CDS             3664..4893
                     /gene="argJ"
                     /locus_tag="PKN51_09485"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNT85370.1"
                     /translation="MPVHLNAPDPTQLHPVAGVRIGVAEAGIRKAGRKDLTVMLLDEG
                     ASIGGVFTQNRFCAAPVQICRQHLQAGAAVRAIVVNTGNANAGTGDDGLARARRSCQA
                     LAGLLQLRPEQVLPFSTGVIMEPLPIERIEAGLPAAIADARPAHWARAAEGIMTTDTV
                     PKAASARARVGGVDITVTGIAKGAGMIRPNMATMLGFVATDAAIDPALMTALARELAD
                     GSFNRITIDGDTSTNDALLVIATHRAGNAPVTALDSADGQALKAALLSVARQLAQAIV
                     RDGEGATKFITVRVEGGQTAGECRQVAYAIAHSPLVKTAFFASDPNLGRILAAVGYAG
                     IADLDQTRIDLYLDEVHVATRGGRHPDYREEDGQRVMKQQEITVRVVLGRGGAADTVW
                     TCDLSHDYVSINADYRS"
     gene            4992..5858
                     /locus_tag="PKN51_09490"
     CDS             4992..5858
                     /locus_tag="PKN51_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT85371.1"
                     /translation="MNQELVRLIERAEQLISRIESVLPQPLSAPDWTQAVAWRYRQRA
                     SGHGALEPVRHVGAITLADLKEIDVQKDKIERNTRQFVEGRPANNVLLTGARGTGKSS
                     LIKACLNAFAPQGLRLIEVDKQDLTDLPDIVEVVAGRPEKFIVYCDDLSFDEGEPGYK
                     ALKSILDGSVAASTPNVLVYATSNRRHLLPEYMKDNLSYTHTDDGEVHPGEVVEEKIS
                     LSERFGLWVSFYPFSQDEYLAIAAQWLGALGATPAQIAAARPEALVWALERGSRSGRV
                     AYQFARDYMGQH"
     gene            6125..6592
                     /locus_tag="PKN51_09495"
     CDS             6125..6592
                     /locus_tag="PKN51_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNT85372.1"
                     /translation="MSPEPNPVLTADGHLQRAQAGARSVVDVAVGVLQRPDGRFLLTS
                     RPAGKVYAGYWEFPGGKLEPGESVEAALARELHEELGLDIDPADVRRWREQLVDYPHA
                     LVRLHFCRLYRWGGTLQMREGQAFGWERLPVRVAPVLPGALPVLDWLQVERTA"
     gene            complement(6649..6888)
                     /locus_tag="PKN51_09500"
     CDS             complement(6649..6888)
                     /locus_tag="PKN51_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNT85373.1"
                     /translation="MPPPDAAPPSLPPRSVRCPACGGVSRYAADNPYRPFCSARCRQH
                     DLGAWASESFRLEAQEADGTDQAAPAHPSAPRFPQ"
     gene            complement(6909..7664)
                     /gene="zapD"
                     /locus_tag="PKN51_09505"
     CDS             complement(6909..7664)
                     /gene="zapD"
                     /locus_tag="PKN51_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HNT85374.1"
                     /translation="MILYEYPFNERIRTYLRLEHLFGRLAQLMPRDAPLDHHFALITL
                     FEALDVCARSDLKSDVLKDLDKQKTHLASFRGNPAVSEASLETMLASLEEAFEQLNQQ
                     HGKPGHELTDNEWLMAIRSRAVIPGGTCGFDLPAYHAWQHGQAAARRTELHRWAATLA
                     PMARAIALLLQILRDSGAPQKVMASAGQFQQTLPQGRSFQLLRLRIDASTQLIPEISG
                     NRLMVSVRLMRQDADGKLQLAREDAAFELALCL"
     gene            complement(7693..8301)
                     /gene="coaE"
                     /locus_tag="PKN51_09510"
     CDS             complement(7693..8301)
                     /gene="coaE"
                     /locus_tag="PKN51_09510"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868017.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNT85375.1"
                     /translation="MALRIGLTGGIGSGKSTVAAMLVEQGAALIDADAISRACTAAGG
                     AALPAIAREFGPQLIAADGALDRAAMRELVFRDPGARQRLEAIVHPLVGAETERQARQ
                     ALAQGRRLLVFDVPLLVESIERWRQRVDRIVVVDCEPETQIARVMARNQLPRAQVEQI
                     LAAQASRAQRLAVADAVIFNGADVTLQALRAQVRQLVDAFGI"
     gene            complement(8304..9146)
                     /locus_tag="PKN51_09515"
     CDS             complement(8304..9146)
                     /locus_tag="PKN51_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNT85376.1"
                     /translation="MPDSLVVMAAVGGLFGLLVGSFLNVVIHRLPQMMERQWAAECAE
                     LNGQPAAPQAPFNLLVPRSRCGHCGHPIRWYENIPVLSYLALRGKCSACGTPIGLRYP
                     LVELATGLLFGWCVWRWGWSWTAAAWCLFAAMLVALTLIDWDTTLLPDDLTLPLLWLG
                     LIAATLGLTGVRLSDAVWGAVAGYLSLWLVYWGFKLATGKEGMGFGDFKLFAALGAWF
                     GWQALVPIILMASVIGALVGIGMKLLSALREGGVVPFGPFLALGGITAMVLGPQTILR
                     AVGL"
     gene            complement(9146..10369)
                     /locus_tag="PKN51_09520"
     CDS             complement(9146..10369)
                     /locus_tag="PKN51_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT85377.1"
                     /translation="MATQVAGAKGVKEFVFEWEGKDRNGRVVRGETRAGGENQVQATL
                     RRQGIFVTKIKKRRMRAGKRIKPKDIAIFTRQLATMMKAGVPLLQSFDIVARGNPNPS
                     VTKLLNDIRADVETGTSLSAAFRKYPLYFNSLYCNLVEAGEAAGILEDLLDRLATYME
                     KTEAIKSKIKSALMYPIAVIVVAFVVVAIIMIFVIPAFKEVFSSFGADLPAPTLFVIA
                     MSEFFVQWWWLIFGGLGFGVYFFMQAWKRNEKMQMFMDRLLLKIPIFGELIDKAVVAR
                     WTRTLSTMFAAGVPLVESLDSVGGAAGNSVYKIATDKIQTEVATGTSLTAAMTNANVF
                     PSMVLQMTAIGEESGALDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVVLGTVIGGI
                     VVSMYLPIFKLGQVV"
     gene            complement(10409..12148)
                     /gene="pilB"
                     /locus_tag="PKN51_09525"
     CDS             complement(10409..12148)
                     /gene="pilB"
                     /locus_tag="PKN51_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNT85378.1"
                     /translation="MAIADPVSKTSQPAALPGVGRALVMAGKITQQAADDAFRKAKAA
                     NRPFIAELIQSGAISAAELAHTMSQAFSAPLLDVDALDIEHLPKGLLDPKLSAHHRIV
                     ALNKRGSRLIIATADPSDHEAAEKVKFATQMGIEWVIGEHDKLSRLIEVLGKSLSDAM
                     DSIIGGDFEFDEASLASDAQTEDSDTTQTEVEDAPVVKFLHKMMVDAVNMRASDLHFE
                     PYEHQYRVRFRIDGELREIASPPTAIKEKLASRIKVLSRLDISEKRVPQDGRMKLKMG
                     ADRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYEPEEKERLLKAIERPYGMI
                     LVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAVA
                     LRAFLRQDPDIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPATLTRMRNMGIP
                     PFNIASSVILITAQRLARRLCTQCKAPVDIPRETLREAGFADNELDGSWTPYGPVGCA
                     ACHNGYKGRVGIYEVMPISEEIQRIILADGSELEIAEQARREGVRSLRAAGLHKVKTG
                     VTSLEEVLGVTNQ"
     gene            12435..13547
                     /locus_tag="PKN51_09530"
     CDS             12435..13547
                     /locus_tag="PKN51_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNT85379.1"
                     /translation="MGTITKRGELQWQAKVRRKGYPAQSRTFSYKEDAEKWVRDLERA
                     ADTGGMVDRREAEKNTLDDILRRYKTEVTPSKKSADIESVKIDVILKDATLPKLKMSA
                     VTSSAVASWRDRRLKQVSGATVNREIDVLSTVFNHARREWDVHVENPIPLIKRPDKAR
                     ARDRRFSAEEEAYLLAALTGGERLADGTFSKGARNPWLLPLVKLAIETAMRRGELLSL
                     IWENVDLKRQTAHLPDTKNGDPRTVPLSTRAVAILTELPLPEIDDEGRDAAEPLRTGA
                     VFPTTALALRKGFARAIERAQEQYRADCQQAKRRPVAGFLEDVHFHDTRHEAASRLAE
                     RLTNVLELSAVTGHKDLRMLKRYYHPRAEDLAKKLG"
BASE COUNT         2375 a         4621 c         4630 g         2147 t
ORIGIN      
        1 cgcgcggcgg catcgcgcag cgccatcacc tcggcatgcg cctggccgac cggctgcgtg
       61 aagccttcgc cgagcaccgt ggcggcggcg tccaccagca cgcagccgac cgccgggttg
      121 ggcggcgaca ggcgccgacc gcgctcggcc agcgtcagcg cgcggcgcat catggcgtgg
      181 tcaagggcgc tcagcatggt cgccgcccgt cagcgcaggg gctgcgccac cttgacgcgc
      241 agcgtggata ccgggtggcc ggccgccgcc agcgccgcct gcagcgccgg cgccagctgg
      301 cgcagcttgg ccgccgccgc ctggtgcggc gccagcagcg tccaggcgtc gccctcgggc
      361 gcgccggcct gcaccagcgg ccgcagtgcc ggcggcagca gtggccgcag caccgccagg
      421 cgggcggccg attcggtggc cagccggctc aggtgggcca gcgtgggcgt ctcggccacg
      481 gcctgttgca gcgagatggc ggggcgggtg aaagtgggca tcgacaaggg caaaaaccgc
      541 agcgtggcgg gcattatccg cgccgctggc gggcagctaa aatgcttggt tgccacgcga
      601 aacaccgggc gcgctgcctg cggggccttc cgcaggtggt cgctgccggc cgggttcgcg
      661 tcattttgac ctttttggga cagtcgatca ccgtgcggtg cgccagtggg gcgtcgcggt
      721 gaccgcgcac gcatggccac catcctcacc aagattttcg gcagccgcaa cgaccgcctg
      781 ctcaagcaat accgcaagac cgccgccgcc atcaacgcgc tggagccggc gctggagaag
      841 ctgagcgacg acgagctgcg cgccaagacc gacgaattcc gtcagcgcct ggcgggcggc
      901 gaaagcctgg acaagctgct gcccgaggcc tttgcggtgg tgcgcgaagg ctccaagcgt
      961 gtcatgaaga tgcgccactt cgacgtgcag ctggtcggcg ccatggcgct gcacgagggc
     1021 aagatcgccg agatgcgtac cggcgagggc aagaccctga ccgccacgct ggcggtgtac
     1081 ctgaacgcgc tgtcgggcaa gggcgtgcac gtggtcaccg tgaacgacta cctggcgggc
     1141 cgcgacgccg aatggatggg ccggctgtac aacttcctgg ggctgacggt gggggtcaac
     1201 ctgccgcaga tggcgcgcgc ggacaagcag gccgcctacc aagccgacat cacctacggc
     1261 accaacaacg aattcggctt cgactacctg cgcgacaaca tggtgtacga gccgggcgag
     1321 cgcgtgcagc gcccgctcaa ctacgccatc gtcgacgagg tggactccat cctgatcgac
     1381 gaggcgcgca cgccgctgat catcagcggc caggccgagg accacacggc gctgtacctg
     1441 gcgatcaatg ccgtggtgcc gctgctgacg cgccaggagg gcgaggccga cccgcgcacc
     1501 ggcgagggcg tgaccaagcc gggcgacttc accgccgacg agaagtcgca ccaggtgttc
     1561 ctgaccgaac agggccacga aaccgccgag cgcgtgctgg ccgagcgcgg cctgatcccg
     1621 cccggcgcca gcctgtacga cgcggccaac attacgctga tgcaccacct caacgccgcc
     1681 ctgcgcgcgc accacctgtt ccaccgcgac cagcattacg tggtgcagca gggcgagcat
     1741 ggcccggaga tcgtgatcgt cgacgaattc accgggcgcc tgatgagcgg gcgccgctgg
     1801 agcgacggcc tgcaccaggc ggtcgaagcc aaggaaggcg tgccgatcca ggccgagaac
     1861 cagaccctgg cctcgatcac gttccagaac tacttccgcc tctaccggaa gctggccggc
     1921 atgaccggca ccgccgacac cgaagcctac gaattccagg acatctacgg cctcgaaacc
     1981 gtggtgatcc cgcccaacaa gccgagccgg cgcgacgacc agctcgaccg cgtctataag
     2041 agcacgcagg aaaagtacga cgccgccatc accgacatcc gcgagtgcca cgccaaaggg
     2101 cagccggtgc tggtgggcac cacctcgatc gagaactccg aaatcatctc ccacctgctg
     2161 cagaaggaaa agctgccgca ccaggtgctc aacgccaagc agcacgcgcg cgaggccgac
     2221 atcgtggccc aggccgggcg cccaggcatg atcaccatcg ccaccaacat ggccggccgc
     2281 ggcaccgaca tcgtgctggg cggcaacgtc gaaaaggcgg tgcaggcgct ggaggccgac
     2341 gcctcgatcg acgaggccac gcgccaggcg cgcgccgccg agctgcgcgc gcagtggcag
     2401 caggaccatg agctggtcaa gtcgctgggc gggctgcgca tcatcgccac cgagcggcac
     2461 gaaagccgcc gcatcgacaa ccagttgcgc ggccgctcgg gccgccaggg cgacccgggc
     2521 tcctcgcgct tctacctcag cctggacgac gcgctgatgc gcatcttcgc cggcgaccgc
     2581 gtgcgcgcca tcatggacaa gctgaagatg cccgagggcg aggccatcga agccggcatc
     2641 gtcacgcgca gcatcgaaag cgcgcagcgc aaggtggagt cgcgcaactt cgacatccgc
     2701 aagcagctgc tggagtacga cgacgtctcc aacgaccagc gcaaggtgat ctaccagcag
     2761 cgcaacgaaa tcctcgacgc cagcgacctg tcggcccaga tcgccagcct gcgcgaaggc
     2821 tgcttcaccg acctggtgcg ccagttcgtg ccggccgaat cggtcgagga gcagtgggac
     2881 atcgccggcc tggaaaaggc gctggccgac gactggcaca tcgagctgcc gctgcgccag
     2941 cagatcgagt cgtcctccgc catcagcgac gaggacatcc tcgagcgcgt gctcaaggcc
     3001 gccgacgaag cctacgccgc caaggtggcg caggtcggcg ccgagaactt cggccccttc
     3061 gagcgcgccg tgctgctgca ggccatcgac acccactggc gcgaacacct gtcggcgctc
     3121 gactacctgc gccagggcat ccacctgcgc ggctatgccc agaagcagcc caagcaggaa
     3181 tacaagcgcg aagccttcga gctgttcggc cagctgctgg acgtgatcaa gaacgaggtc
     3241 acgcgggtgc tgatgacggt gcgcatcgaa agccccgaac aggccggcca gagcgccgcc
     3301 gccatggagc agcgcgccga gcacatcagc aacgtcacct acaccgcgcc caccgaaacc
     3361 ggcgaggccc agaccgtgac cgaagcgcag gcgcgcggcg ccgccgccgc ggccggtggc
     3421 gaggtggcgg cggcggcggc ggcggtgcca cgcgtcggcc gcaacgaccc ctgcccgtgc
     3481 ggcagcggca agaaatacaa gcagtgccac ggcgcgctca cttgaccgct gctgccggcg
     3541 ccgccccggt tcgggcacct gcggaacacc gccagaagct atttatttga tagctgctgg
     3601 cgcttgaccg acaagcgcta gcggcctatg ggattcacaa tctgccgatc gaaggaacct
     3661 gtcatgccgg tccacctgaa cgctcccgat cccacgcaac tgcacccggt cgccggcgtg
     3721 cgcatcggcg tggccgaagc cggcatccgc aaggccggcc gcaaggacct gaccgtgatg
     3781 ctgctcgacg aaggcgccag catcggcggc gtgttcacgc agaaccgctt ctgcgccgcg
     3841 ccggtgcaga tctgccgcca gcacctgcag gccggcgccg cggtgcgcgc catcgtggtc
     3901 aacaccggca acgccaacgc cggcaccggt gacgacggcc tggcgcgcgc gcgccgcagc
     3961 tgccaggcgc tggccgggtt gctgcagctg cggcccgagc aggtgctgcc gttttccacc
     4021 ggcgtcatca tggagccgct gccgatcgag cgcatcgaag ccggcctgcc ggccgccatc
     4081 gccgacgcgc ggcccgccca ctgggcgcgc gccgccgaag gcatcatgac caccgacacc
     4141 gtgcccaagg ccgccagcgc ccgtgcccgc gtgggcgggg tcgacatcac cgtcaccggc
     4201 atcgccaagg gtgccggcat gatccgcccc aacatggcca ccatgctggg cttcgtggcc
     4261 accgacgccg ccatcgaccc ggcgctgatg accgcgctgg cgcgcgaact ggccgacggc
     4321 tcgttcaacc gcatcaccat cgacggcgac acctcgacca acgatgcctt actggtcatc
     4381 gccacccacc gcgccggcaa cgcgcccgtc accgcgctcg attcggccga cggccaggcg
     4441 ctgaaggcgg cgctgctgtc ggtggcgcgc cagctggccc aggccatcgt gcgcgacggc
     4501 gagggtgcca ccaagttcat caccgttcgc gtcgaaggcg gccagacggc cggcgagtgc
     4561 cgccaggtgg cctacgccat cgcccattcg ccgctggtca agacggcgtt cttcgccagc
     4621 gaccccaacc ttggccgcat cctggccgcc gtcggctacg ccggcatcgc cgacctcgac
     4681 cagacgcgca tcgatctcta cctcgacgag gtgcacgtgg ccacgcgcgg cggccgtcac
     4741 cccgactacc gcgaggaaga cggccagcgc gtgatgaagc agcaggaaat caccgtgcgc
     4801 gtggtgctcg gccgcggtgg cgccgccgac accgtgtgga cctgcgacct cagccacgac
     4861 tacgtcagca tcaacgccga ctaccgctcg tgagccgtcg gccggtcggc ccgacgggcc
     4921 ggcggcgaaa gccgtcgtta ctattgaatg gatagctatt ggcgctgatc caccaagcgc
     4981 tggatgccga aatgaatcag gaactcgtgc ggctgatcga acgggccgaa caactcatct
     5041 cccgcatcga atcggtgctg ccgcaaccgc tgtcggcgcc cgactggacg caggccgtgg
     5101 cctggcgcta ccgccagcgc gccagcggcc acggcgcgct ggagccggtg cgccacgtcg
     5161 gcgccatcac gttggccgac ctcaaggaaa tcgacgtcca gaaggacaag atcgagcgca
     5221 acacacgcca gttcgtcgaa gggcggccgg ccaacaacgt gttgctgacc ggggcgcgcg
     5281 gcaccggcaa gtcctcgctc atcaaggcct gcctgaacgc ctttgcgccg caggggctgc
     5341 gcctgatcga ggtcgacaag caggacctga ccgacctgcc cgacatcgtc gaggtggtgg
     5401 ccggccggcc tgagaagttc atcgtctatt gcgacgatct cagcttcgac gagggcgagc
     5461 cgggctacaa ggcgctcaag agcattctgg acggcagcgt ggccgccagc acccccaacg
     5521 tgctggtcta cgccaccagc aaccgccgcc acctgctgcc cgagtacatg aaggacaacc
     5581 tcagctacac ccacaccgac gacggcgaag tgcatccggg cgaggtggtg gaggagaaga
     5641 tctcgctgtc cgagcgcttc ggcctgtggg tcagcttcta tccgttcagc caggacgagt
     5701 acctggccat cgcggcccag tggctgggcg cgctgggcgc cacgccggcg cagatcgccg
     5761 ccgcgcgccc cgaggcgctg gtgtgggcgc tcgaacgtgg ctcgcgctcg ggccgggtgg
     5821 cgtaccagtt cgcgcgcgac tacatgggcc agcattgagc ctggaagccg cagtggaccg
     5881 cttgcaaccc ttgggaatgc cgcatgaacg ctgaccttgc gccacttggg agccttcacc
     5941 ttctgggtga aggcgctagg caccggccag caccgctgct gggcccgttc gcgccgtccc
     6001 aaggcgcgca ggcgcctttg gagccgcggc gctcaccctt gcctggcaca ccgatcacgg
     6061 cgctggcagg cgcctccaac ggccgtatct aggttgagcc gccggcggct gcaccggtca
     6121 cgccatgagc ccagagccga accccgtgct gaccgccgac ggccacctgc agcgcgcgca
     6181 ggccggtgcg cggtcggtgg tcgacgtggc ggtcggcgtg ctgcaacggc cggacgggcg
     6241 ctttctgctg acctcgcgcc cggccggcaa ggtgtatgcg ggctattggg aatttccggg
     6301 gggcaagctg gagccgggcg agagcgtcga ggcggcgctg gcgcgcgagc tgcacgagga
     6361 gctgggtctg gacatcgacc cggccgacgt gcggcgctgg cgcgagcagc tggtggacta
     6421 tccgcatgcg ctggtgcggc tgcatttctg ccggctgtac cggtggggcg gcacactgca
     6481 gatgcgcgag ggccaggcct ttggctggga gcggctgccg gtccgcgtgg cgccggtgct
     6541 gccaggcgct ctgccggtgc tggattggct gcaggtcgag cgcacggcct gagaatgcta
     6601 ttgtttatat agctggcggc gcttgtcagg aaagcgccgg agccctgttc attgcggaaa
     6661 ccggggtgcc gatggatggg cgggtgcagc ttggtcggtg ccgtccgctt cctgggcctc
     6721 gaggcgaaag ctctcgctgg cccaggcgcc caggtcgtgc tggcggcagc gcgcgctgca
     6781 gaacgggcga tacgggttgt cggcggcgta gcggctgacg ccaccgcagg cagggcagcg
     6841 cacgctgcgg ggcggcaggc tgggcggggc ggcgtcgggc gggggcatgg ggcgggcgca
     6901 acacgggatc agaggcacag tgccagctcg aaggccgcgt cctcgcgcgc cagctgcagc
     6961 ttgccgtcgg cgtcctgccg catcaggcgc accgacacca tcaggcggtt gccgctgatc
     7021 tcggggatca gctgcgtcga tgcatcgatg cgcaggcgca gcagctgaaa gctccggcct
     7081 tgcggcagcg tctgctgaaa ctggccggcg ctggccatca ccttctgtgg cgcgccggaa
     7141 tcgcgcagga tctgcagcag cagcgcgatg gcgcgcgcca tcggcgccag cgtggcggcc
     7201 cagcggtgca gctcggtgcg ccgcgccgcc gcctggccgt gctgccaggc gtggtaggcc
     7261 ggcaggtcga agccgcaggt gccgccagga atgacggcgc ggctgcggat ggccatcagc
     7321 cactcgttgt cggtcagctc gtggcccggc ttgccgtgct gctggttgag ctgttcaaac
     7381 gcctcttcca ggctggccag catggtctcc agtgaggcct cggagacggc cgggttgccg
     7441 cgaaagctgg ccagatgggt cttctgcttg tccaggtcct tgagcacgtc ggactttagg
     7501 tccgaacggg cacacacgtc cagtgcctcg aacagggtga tgagggcgaa gtgatggtcc
     7561 agcggcgcgt cgcgcggcat cagctgggcc agacggccga acaggtgctc cagccgaagg
     7621 taggtgcgga tgcgttcgtt gaaagggtac tcgtacagga tcacgcgtgg cgccttgtca
     7681 gggtcgcccg catcatatcc cgaaggcatc gaccagttga cgcacctggg cgcgcagcgc
     7741 ctgcagcgtc acgtcggcac cgttgaagat caccgcatcg gccaccgcca gccgttgtgc
     7801 gcggctggcc tgcgccgcca ggatctgctc gacctgggcc cgcggcagct ggttgcgcgc
     7861 catcacgcgg gcgatctggg tctcgggttc gcagtcgacc accacgatgc gatccacccg
     7921 ctggcgccag cgctcgatcg actcgaccag cagcggcacg tcgaacacca gcaggcggcg
     7981 cccctgggcc agcgcctgcc gggcctggcg ctcggtttcg gcgccgacca gtgggtggac
     8041 gatggcttcc agccgctggc gcgcgccggg gtcacggaac accagctcgc gcatggcggc
     8101 gcggtccagt gcgccatcgg ccgcgatcag ctgcgggccg aattcgcgcg cgatggcggg
     8161 cagggccgcg ccaccggcgg cggtgcaggc gcgcgagatg gcgtcggcgt cgatcagggc
     8221 ggcgccctgt tcgaccagca tggccgccac cgtgctcttg ccgctgccga tgcctccggt
     8281 caggccgatg cgcagcgcca tggctacagg ccgaccgcgc gcaggatggt ctgcggcccc
     8341 agcaccatgg cggtgatgcc gcccagggcc agaaacggcc cgaagggcac caccccgcct
     8401 tcgcgcagcg cgctgagcag cttcatgccg atgcccacca gcgcgccgat gaccgaggcc
     8461 atcaggatga tcggcaccag ggcctgccag ccgaaccagg cgcccagtgc ggcgaacagc
     8521 ttgaagtcgc caaagcccat gccttccttg ccggtggcca gcttgaagcc ccagtacacc
     8581 agccacagcg acaggtagcc ggccaccgcg ccccacaccg cgtcgctcag gcgcacgccg
     8641 gtcaggccca gggtggcggc gatcaggccc agccacagca gcggcagcgt caggtcgtcg
     8701 ggcagcaggg tggtgtccca gtcgatcagc gtcagcgcca ccagcatggc cgcgaacagg
     8761 caccaggcgg cggccgtcca gctccagccc cagcgccaca cgcaccagcc gaacagcaga
     8821 ccggtcgcca actccaccag cgggtagcgc aggccgatcg gcgtgccgca ggccgagcac
     8881 ttgccgcgca gcgccaggta gctcagcaca gggatgtttt cgtaccagcg gatcgggtgc
     8941 ccgcaatggc cgcagcgcga gcgtggcacc agcaggttga acggcgcctg cggggcggcg
     9001 ggctggccgt tcagttcggc acattcggcc gcccactgcc gctccatcat ctgaggcagg
     9061 cggtggatga ccacgttcag gaagctgccg acgagcagac cgaacaggcc gcccacggcg
     9121 gccatgacca ccagactgtc gggcatcaga ccacctggcc gagcttgaag atcggcaggt
     9181 acatcgacac cacgatgccg ccgatcacgg tgcccagcac gacgatgatg atgggctcca
     9241 tcaggctgga caggccggcc accatctcgt cgacctcggc ctcgtagaag tcggctgcct
     9301 tgcccagcat gtggtcgagc gcgccggatt cctcgccgat ggcggtcatc tgcagcacca
     9361 tgctgggaaa cacgttggcg ttggtcatgg cggcggtcag actggtaccg gtggccacct
     9421 cggtctggat cttgtcggtg gcgatcttgt agaccgaatt gccggcagcg ccgcccaccg
     9481 agtcgagcga ttcgaccagc ggcacgccgg cggcgaacat ggtcgacagc gtgcgcgtcc
     9541 agcgcgccac cacggccttg tcgatcaatt caccgaagat cgggatcttc agcagcagac
     9601 ggtccatgaa catctgcatc ttctcgttgc gcttccaggc ctgcatgaag aagtagacgc
     9661 cgaaacccag cccgccgaag atcagccacc accactggac gaagaactcg ctcatggcaa
     9721 tgacgaacag cgtgggcgcc ggcaggtcgg cgccgaagga gctgaacacc tccttgaacg
     9781 ccggaatgac gaaaatcatg atgatggcca ccaccacgaa ggccaccacg atcaccgcga
     9841 tcgggtacat cagcgccgac ttgatcttgg acttgatggc ctcggtcttc tccatgtagg
     9901 tggccagccg atccagcagg tcttccagga taccggccgc ctcgccggct tcgaccaggt
     9961 tgcagtacag gctgttgaag tacagcgggt acttgcggaa cgccgccgac agcgaggtgc
    10021 cggtttcgac gtcggcgcgg atgtcgttga gcagcttggt gacgctgggg ttggggttgc
    10081 cgcgggccac gatgtcgaac gactgcagca gcggcacgcc ggccttcatc atggtggcca
    10141 gctggcgcgt gaagatggcg atgtccttgg gcttgatgcg cttgcccgcg cgcatgcgcc
    10201 gcttcttgat cttggtgacg aagatgccct gtcgacgcag cgtggcctgg acctggttct
    10261 cgcccccggc ccgggtttcg ccgcgcacca cccggccgtt gcggtccttg ccttcccact
    10321 cgaagacgaa ttccttgacg cccttggcgc cagcgacttg agttgccatg aaatgccctg
    10381 tgttcagtcc tggtcagggg tgccgactct actggttggt cacgcccagc acttcctcca
    10441 gtgacgtcac tcccgttttc accttgtgca gtcccgccgc gcgcagcgag cgcacgcctt
    10501 cgcgccgcgc ctgctcggcg atctcgagct cgctgccatc ggccaggatg atgcgctgga
    10561 tttcttcgct gatcggcatg acttcgtaga tgccgacgcg ccccttgtag ccgttgtgac
    10621 aggccgcgca gccgaccggg ccgtagggcg tccagctgcc gtccagctcg ttgtcagcga
    10681 atccggcttc cctcagtgtt tcgcgcggga tgtccaccgg cgccttgcac tgcgtgcaca
    10741 ggcgtcgcgc cagccgctgc gccgtgatca ggatcacgct ggaagcgatg ttgaagggcg
    10801 gaatgcccat gttgcgcatg cgcgtcagcg tggcgggggc gtcgttggtg tgcagcgtcg
    10861 agagcaccag gtgaccggtc tgcgccgcct tgatggcgat gtcggcggtt tccaggtcgc
    10921 gaatttcacc gaccatgatg atgtccgggt cctggcgcag gaaggcacgc agcgccaccg
    10981 cgaaggtcag tccggcccgc tcgttgacgt tgacctggtt gacgcccggc aggttgattt
    11041 ccgacgggtc ttcggcggtg gcgatgttga cgcccggctt gttgagcagg ttcaggcagg
    11101 tgtagagcga caccgtcttg cccgagccgg tggggccggt gaccaggatc atgccgtagg
    11161 ggcgctcgat ggccttcagc aggcgctctt tttcctcggg ctcgtagccg agggcgtcga
    11221 tgccgagccg ggcgctgctc ggatccagga tacggatcac gatcttttcg ccaaacagcg
    11281 taggcagcgt gctgacccgg aagtcgatca cccggtcggc acccatcttg agcttcatgc
    11341 gcccgtcctg cggcacgcgc ttttcggaga tgtccagacg cgacaggacc ttgatgcgcg
    11401 aggccagctt ttccttgatg gcggtgggcg gcgaggcgat ctcgcgcagt tcgccgtcga
    11461 tgcgaaaccg cacgcggtac tggtgctcgt agggctcgaa gtgcaggtcg gaggcgcgca
    11521 tgttgacggc gtcgaccatc attttgtgca ggaacttgac gaccggcgca tcctcgacct
    11581 cggtctgcgt ggtgtcggaa tcctccgtct gggcatccga cgcgagcgag gcttcgtcga
    11641 actcgaagtc gccgccgatg atgctgtcca tggcgtcgga cagcgacttg ccgagcacct
    11701 cgatcagtcg tgacagcttg tcgtgctcgc cgatcaccca ctcgatgccc atctgggtgg
    11761 cgaacttgac cttttccgcc gcttcgtggt cggacgggtc ggccgtggcg atgatcagac
    11821 ggctgccgcg cttgttgagc gccacgatgc ggtggtgggc cgacagcttg gggtcgagca
    11881 gccccttggg caggtgctcg atgtcgagcg cgtcgacgtc gagcagcggc gccgagaacg
    11941 cctgcgacat ggtgtgcgcc agctccgccg ccgagatggc ccccgactgg atcagttcgg
    12001 caatgaacgg tcgattggcc gccttggcct tgcggaacgc gtcatcggcg gcctgctggg
    12061 tgatcttgcc agccatcacc aacgcccgcc ccacgccggg cagggcggca ggctggctgg
    12121 ttttggagac tggatcggcg atggccatgg gaaaaaacgt cacccaaaag gctaaaagtg
    12181 cctctcaatc atcgctgatc agtgaatttc tgtaaacgat gatcgcccct gaatgtgtgc
    12241 atcaatcgac acattcgcct ccggaccacc gcccagggtg gctgccagaa aagaaaaagg
    12301 tggggaaaag tggtcggggt gagaggattc gaacctccgg cctctacgtc ccgaacgctc
    12361 ggcacttgtc ccgtgtttgc tgttgttcta agctatccct gcattaattg ggacaaattt
    12421 gggacacaag acacatgggc acgatcacga aacgtggtga attgcagtgg caagccaagg
    12481 tgcgacgcaa aggctaccca gcacagtctc gcaccttctc ctacaaggag gacgccgaga
    12541 agtgggttcg tgacctggag cgtgcagccg acacaggtgg catggttgac cggcgcgagg
    12601 ctgagaagaa cacgctggat gacattctgc gccgctacaa gacggaggtc acaccctcca
    12661 agaagtcagc cgatatcgag tccgtcaaga tcgacgtgat tctgaaggac gccacccttc
    12721 ctaagctcaa gatgtcggcc gtcaccagca gcgcggttgc aagctggcga gatcggcgtc
    12781 tcaagcaggt cagtggtgcc accgtcaatc gcgaaatcga tgtgttgtct accgtgttca
    12841 accatgcgcg ccgcgagtgg gatgtacatg tcgagaaccc gattccgctg atcaagcgac
    12901 ccgacaaggc gcgggctcgg gatcgtcgtt tttctgccga ggaagaggcc taccttctgg
    12961 ccgcattgac gggtggtgag cgcttggccg atggtacgtt cagcaagggc gcgcgcaacc
    13021 cctggctgct gccattggtc aagttggcaa ttgaaaccgc gatgcgacgt ggcgaactgc
    13081 tgtcgctcat ctgggagaac gtcgatctga agcggcagac cgcccacttg cccgacacaa
    13141 agaacggtga tcctagaacg gtgccgctgt ctactcgggc tgttgccatc ctgacggaac
    13201 tgcctctgcc tgagattgac gatgaaggcc gtgacgcagc agaacctctg cggaccggag
    13261 ctgtgttccc aacgacggcg ttggcattgc gcaaaggctt tgcgcgggct attgagcgcg
    13321 cgcaggagca ataccgtgct gactgccaac aagcaaaacg ccgtcctgtc gcgggcttcc
    13381 ttgaagacgt tcactttcac gacacacgcc atgaggcggc atcgcgcctg gccgagaggt
    13441 tgacgaacgt tctggagctg tctgcggtca ctggccataa agacttgcgc atgctcaagc
    13501 gctactacca cccacgtgct gaagacttgg cgaagaagct gggttagacg accagcgagg
    13561 gttgtgtgga taaggctgtg ttctgtgcgt gttgtcctcg ttatatcgcc ccttgatggt
    13621 tcgatacatc gcccctgatg gtgttctgca ggcgacttcg ttatatcgcc ccttgtgggc
    13681 tcgttatatc gccccttctc tatacctata gggtgttcct atagatttct tataactttg
    13741 ctagtagcgc ttgctgtatc ggagggatga tac
//
LOCUS       DAOQTZ010000075        13757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5024_length_13757_cov_13.438622, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000075 DAOQTZ010000000
VERSION     DAOQTZ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13757
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5024_length_13757_cov_13.438622"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /gene="groL"
                     /locus_tag="PKN51_09535"
     CDS             complement(<1..1150)
                     /gene="groL"
                     /locus_tag="PKN51_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655678.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNT85380.1"
                     /translation="MAAKDVVFGGEARARMVEGVNILANAVKTTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKLQNMGAQMVKEVASKTSDNAGDGTTTATVLAQAIVRE
                     GMKYVAAGMNPMDLKRGIDKAVAALVEQLKKASKATTTSKEIAQVGSISANSDESIGK
                     IIADAMDKVGKEGVITVEDGKSLDNELDVVEGMQFDRGYLSPYFINNPEKQAAILDNP
                     YVLLFDKKISNIRELLPTLEAVAKAGRPLLIIAEEVEGEALATLVVNTIRGILKVVAV
                     KAPGFGDRRKAMLEDIAILTGGKVIAEEVGLSLEKVTLADLGQAKTIEVGKENTTIID
                     GAGKAADIEARVKQIRVQIEEATSDYDREKLQERVAKLAGGVAVIKVGA"
     gene            complement(1253..1543)
                     /gene="groES"
                     /locus_tag="PKN51_09540"
     CDS             complement(1253..1543)
                     /gene="groES"
                     /locus_tag="PKN51_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNT85381.1"
                     /translation="MKLRPLADRVIVKRLENETKTASGIVIPDTAAEKPDQGEVMAVG
                     PGRLDEDGERMPMSVKVGDRVLFGKYSGQTVKVDGDELLVMKEDDLFAVVEK"
     gene            1852..3399
                     /locus_tag="PKN51_09545"
     CDS             1852..3399
                     /locus_tag="PKN51_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT85382.1"
                     /translation="MNPNTDPVDYALARQAAGVTIGALFARCVRRAPGRIALQQGPHG
                     LTYAQLDERSNRLCQHLAGLGIGRGDRVAILSENRTEYVELLIAAAKLGAIAACQNWR
                     LRDEELAHCLALVTPRLILASPRHAEAARRCGGAVPLLCLGAEYEQALQAATAEPVED
                     RCDPEDGVVILYTSGTTGMPKGALISQRAEIARAAIQAMDLPHSADEHFVAWAPLFHM
                     VSTDTVFMTLMQGGKVIVTDGFDADELAAIVARERLGRLTLMPGMITPFLQAMQRLGA
                     RPAGVKWVGVMADLVPREQIAEVTRLLGAPYINSFGATETGYPPASLATIPVGVVPTS
                     LDKQQSSLCRIRLVDADDREVPDGEPGELTIRTPALFSGYWNNPDANREDFRGGWFHM
                     GDVFRRNPDGTLSFVDRRKYLIKSGGENIYPAEIERVLLSEPRVADAVVVRRPDPTWG
                     EVPVAFVVRRDPTLDAAALQALCRQQIAGYKVPKEVHFVDDAALPRSTTGKIMRHELE
                     QRLPPVA"
     gene            complement(3404..4444)
                     /locus_tag="PKN51_09550"
     CDS             complement(3404..4444)
                     /locus_tag="PKN51_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNT85383.1"
                     /translation="MSEISEAAAGVRGWPRQLRRLSPGLVVALIVALAATFVSEHYGG
                     PKFLYALLMGMALHFLSEDARCGPGIEFAARQLVRLGVALLGARIAFGDVQALGGAGV
                     AWLAAAVALTIGFGVALARALGLPARFGLISGGATGICGISAALAISSALPRDGRTEQ
                     QTLMTAIGVAALSTAAMVLYPLVAQALQLDARLAGLFLGGAIHDVAQVVGAGAILSPE
                     VAQSATLVKMFRVAMLVPVVLCVALAFRAPAHAAGQPRPRGPALLPGFLLAFIALVVA
                     NSLGAIPVPLQSAAAGVSAWALVVAIAALGVKTSFEKIVRLGWRPIALLVSETLFIAL
                     FMLLAVGWQARS"
     gene            complement(4510..6408)
                     /gene="glmS"
                     /locus_tag="PKN51_09555"
     CDS             complement(4510..6408)
                     /gene="glmS"
                     /locus_tag="PKN51_09555"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNT85384.1"
                     /translation="MCGIVGAVSTRNIVPILVQGLQRLEYRGYDSCGVSVHSAGLGAP
                     TGALRRARGITRVADLAAQCEAEGLQGLTGIAHTRWATHGAPAVANAHPHFSMGPHAD
                     DGAERPGRVTLVHNGIIENHEALRAELLARGYHFSSQTDTETIVHLIDSLYDGDLLTA
                     VQTAVRRLRGSYSIAVMHRDEPQRLIGAREGSPLVLGIGADGKENFLASDAMALAGVT
                     DQIVYLEEGDLIDVQLGKYWVLDRAGQRAERPVRTVQAMSGAADLGPYRHYMQKEIFE
                     QPRAVADTLEGVQAVVPQLFEAAGTPAPGEDAQTTFQAVRQVLILACGTSYYAGCVAK
                     YWLEDIAGIPTQVEIASEYRYRASVPDPATLVVTISQSGETADTLAALRHAQRLGMRH
                     SLTVCNVPTSAMVRECRLAFLTRAGVEIGVASTKAFTTQLVGLFLLALMLAKTRARLG
                     PAQEAEQLDALRRLPGALQAVLALEPQLIDWAEQFTAKEHALFLGRGLHYPIAQEGAL
                     KLKEITYIHAEAYAAGELKHGPLALVTSAMPVVTVAPNDALLEKLKSNMQEVRARGGV
                     LYALADADTHIESSDGLYVIRMPGRSGLLSPILHVVPLQLLAYHTAVARGTDVDKPRN
                     LAKSVTVE"
     gene            6568..7083
                     /locus_tag="PKN51_09560"
     CDS             6568..7083
                     /locus_tag="PKN51_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNT85385.1"
                     /translation="MELDDVDWRLLDALQHDASLSNQALAERLQLSPPTALRRVRRLR
                     QAGLIAAQVALLDEDRVAAAQGHGLTAIAEVSLDRQGAEHLDAFERRAVQDTAVQQCW
                     RVAPGPDFVLVLRVRDMPHYQQLAERLFTQDANVRNVRVFFATRRAKFQTQVPLARPG
                     LPTAPRVQVPD"
     gene            complement(7068..7760)
                     /locus_tag="PKN51_09565"
     CDS             complement(7068..7760)
                     /locus_tag="PKN51_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNT85386.1"
                     /translation="MPIAPSRRHAFWLVLMLLAASVATAASVAGAVGLHRASKPLVML
                     FAICLVAGRADLSGVGTRFRWILMLALACSLAGDVFLMFEGLFLPGLVAFLLAHLAYL
                     ALFLRGLPWLPSRAAALAVALIGAAMYVFLWRGGLPAALRLPVALYVLAIGLMVAQAV
                     GRALRLRDGAAWRVALGAGLFMLSDSLLATNRFVQPLPLAPLWVLGSYFIAQLLIAAC
                     ARPVSARGQSGT"
     gene            complement(7775..8950)
                     /locus_tag="PKN51_09570"
     CDS             complement(7775..8950)
                     /locus_tag="PKN51_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HNT85387.1"
                     /translation="MNASVDPVAPRLRALIGQGLDAIDTPALVVDLDAANRNLQRMVE
                     FATRYRLRLRPHAKMHKSAQFARLQLQAGAVGLCVQTVGEAEALAAGGINDIFITNEL
                     LAVGKLRRVAALAHQLATRGGQLAIAVDSSEGIQRLAQAMRLTRAVIDVFIEIDIGQG
                     RCGVPPGDAAVALARQLSDASSSLRYAGLQAYHGGVQHLRGVAERRQALAAAAERLRT
                     TCAALAAAGLAPPLVSGAGTGSFGIEAASGLWGEIQPGSFLFMDADYLRNERDPAQPQ
                     FEPALFVKAQVISANAERVVCDAGHKSHAIDSGLPLVHALPGQPALHYANGGDEHGIL
                     RPAAPGGALPALGDTLWLIPGHCDPTVNLHDHLIVVRGGLAAGSVDTVVPVDARGAW"
     gene            complement(8934..9584)
                     /locus_tag="PKN51_09575"
     CDS             complement(8934..9584)
                     /locus_tag="PKN51_09575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNT85388.1"
                     /translation="MQATRILAVRHGETAWNVDGRIQGHLDIALSDVGRAQAHRLGRA
                     LAEREPLALIVSSDLARARETARIVADATGAPLRTTTALRERCFGDLQGQRFAEIEAS
                     WPEQAERWRRREPDWAPPGAGGESLLQFRERVRQAVQALAAENMGNHIALFTHGGVLD
                     VLYRLATGLGLQDARSWQIGNTAVNRLLWTPDSGLSLVGWADTTHLEDESLDERFS"
     gene            9715..10944
                     /locus_tag="PKN51_09580"
     CDS             9715..10944
                     /locus_tag="PKN51_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT85389.1"
                     /translation="MTAPHRDASGAWTALLLLATGFLMSQAFRTIAAIMAAPLQAELQ
                     LTPQQLGTFAAAYHFAFGGLQLFMGMGIDLHGLRRTVLTASPLMALGAAVSALATGFP
                     MLVAGQVLIGIGSAPAFLVCTIYVARHFAPERYTVVSSLILAVGGIGMLLTGSPLAWL
                     IEATSWRMGFWVLAGCALLSWLAIALGVREPAPATGARRETLGQALRTFGALLRQRHT
                     AGIVALALVSYGAFITLRGLWLGPLMTQRHGWSLVAAGHLAVLLTVVSLVSLPLFGRA
                     DPGPRRRRRWMLWGSAVAALCFAGLAANGGMWLDVALMLVFMMLSGYGTLQYPDVKDA
                     YEPAVIGRAMALFTMALFMGVALMQWATGAIAQAAQARGLDPFGAVFGAIAVLLALGA
                     LAFRLLPQPASVRTDVE"
     gene            complement(10992..11636)
                     /locus_tag="PKN51_09585"
     CDS             complement(10992..11636)
                     /locus_tag="PKN51_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85390.1"
                     /translation="MWIARAALVLIASALTACASGPRVVDAHVRTHAAQPPGAAVLQG
                     AHYRFEPGPQRAGQPAPARLEAMAQAALTRVGAVRDEGGARVSVQVSGNVNALWPDPW
                     YEPSNARIALGLGHAWRGGAVGLGWGWPLGDPSVPTYASEVSLLMRDLQTGQIVYDTR
                     ARHHGPWHDTDAVLAALFVAALEGYPQPAASERRVGVPLLPPATPAAAPAATGR"
     gene            complement(11668..12591)
                     /gene="ttcA"
                     /locus_tag="PKN51_09590"
     CDS             complement(11668..12591)
                     /gene="ttcA"
                     /locus_tag="PKN51_09590"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNT85391.1"
                     /translation="MIEEPVVADTGPAARTRAEREALKLEKRLARQVGQAIADFHMIE
                     AGDRVMVCLSGGKDSYGLLDILLKLKARAPIHFDLVAVNLDQKQPGFPAEVLPNYLSG
                     LGIEHHIETQDTYSIVKAKIPEGKTTCSLCSRLRRGILYRVARQLGCNKIALGHHRDD
                     MLQTLFLNMFFGGKLKGMPPKLVSDNGEFIVIRPLAYVAESDLVAWAEHRQFPIIPCN
                     LCGSQENLQRQQVGEMLREWQRRHPGRIESMFTALQNVVPSHLMDARLHDFKHLRPSG
                     VPDEHGDKAFDDEVFVPPVGAPFSSVTRVSA"
     gene            complement(12649..13032)
                     /locus_tag="PKN51_09595"
     CDS             complement(12649..13032)
                     /locus_tag="PKN51_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNT85392.1"
                     /translation="MPSHPRAEQGQQSLQLTGLRFAANLGILAHEKLAPQPIQVDAEL
                     HLGAQPLRPRDDDITHVLDYRKVRQIIIDECTAEHVNLLETLIGKLCARLMQLPGVQG
                     VRVKITKLEIFDDCEVSIHMEAGHW"
     gene            complement(13041..13454)
                     /locus_tag="PKN51_09600"
     CDS             complement(13041..13454)
                     /locus_tag="PKN51_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNT85393.1"
                     /translation="MNSLLLAFGALDARLALDCRLLSLRRHELPVRIGVHEFERQGPQ
                     RLWFDVDLCVRLRDAAAAHDHIAHTLDYDFVRDVIAAAVGQAHHELQETLCDAVLAAV
                     LARPEVQAARVATRKPDVYPDSASVGVERVAVKPW"
     gene            complement(13451..>13757)
                     /locus_tag="PKN51_09605"
     CDS             complement(13451..>13757)
                     /locus_tag="PKN51_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HNT85394.1"
                     /translation="KLALAQSVALQAQALAPWVRVCGLSPGLMYLSGPQTQDNFERAS
                     RVNLLREPLDPAQVARCVVFIAETPALSGTTLQADNGQHLVPLARDVMFAVDEGLTR"
BASE COUNT         1957 a         4758 c         4899 g         2143 t
ORIGIN      
        1 cggcgcccac cttgatgacg gccacgccgc cggccagctt ggccacgcgc tcctggagct
       61 tttcgcggtc gtagtcgctg gtggcttcct cgatctgcac gcggatctgc ttgacgcgcg
      121 cctcgatgtc ggcggccttg ccggcgccgt cgatgatggt ggtgttttcc ttgcccactt
      181 cgatggtctt ggcctggccc aggtcggcca gcgtcacctt ctccagcgac aggcccactt
      241 cctcggcgat caccttgccg ccggtcagga tggcgatgtc ttccagcatg gccttgcggc
      301 ggtcgccaaa gccaggcgcc ttgacggcca cgaccttcag gatgccgcgg atggtgttga
      361 ccaccagggt cgccagggcc tcgccttcga cttcttcggc gatgatgagc agcggacggc
      421 cggccttggc cacggcttcc agcgtgggca gcagctcgcg gatgttgctg atcttcttgt
      481 cgaacagcag cacgtagggg ttgtccagga tggcggcctg cttctcgggg ttgttgatga
      541 agtagggcga caggtagccg cggtcgaact gcatgccttc gaccacgtcc agctcgttgt
      601 ccaggctctt gccgtcttcg acggtgatca cgccttcctt gcccaccttg tccatggcgt
      661 cggcgatgat cttgccgatc gactcgtcgc tgttggccga aatcgagccg acctgggcga
      721 tttccttgct ggtggtggtg gccttgctgg ccttcttgag ctgctcgacc agggcggcca
      781 cggccttgtc gatgccgcgc ttgagatcca tcgggttcat gccggcggcc acgtacttca
      841 tgccttcgcg cacgatggcc tgggccagca cggtggcggt ggtggtgccg tcaccggcgt
      901 tgtcgctggt cttggaagcc acttccttga ccatctgcgc acccatgttc tgcagcttgt
      961 ccttgagttc gatctccttc gcgaccgaga caccgtcctt ggtgacagtg ggggcgccga
     1021 aggagcgctc gaggacgacg ttgcggccct tggggcccag ggtggtcttg accgcgttgg
     1081 ccaggatgtt cacaccttcg accatgcggg cgcgggcttc gccgccgaaa actacgtctt
     1141 ttgctgccat ttgctttggc tcctgaaaat gggtaagaaa aacgccgcta ccgcttatcc
     1201 atcaagcgcg agcagctatc aaatgaatag tgttttttgc gaaggtgagg gatcacttct
     1261 cgacgaccgc gaacaggtcg tcttccttca tcaccagcag ctcgtcgcca tcgaccttga
     1321 cggtctggcc gctgtacttg ccgaacagca cgcggtcgcc gaccttgacg ctcatgggca
     1381 tgcgctcacc gtcctcgtcc aggcggcccg ggcccacggc catcacttca ccctggtcgg
     1441 gtttttcggc ggcggtgtcg gggatgacga tgcccgaggc ggtcttggtt tcgttttcca
     1501 ggcgcttgac gatcacgcga tcggccagag gacgcagctt catgggttct cctgaatgca
     1561 agatgggaaa gggttggggc ctgcgccggc ccctgttgcc aggcggccgg ccgaccggaa
     1621 tgcgatgaac cgcggtgcct tgctagcact caagttcaac gagtgctaat gataagggta
     1681 aatggcggcg attcaagagc gccgggtcac ccgggtggcg ctcgcacccc tctggcggcg
     1741 ctgcccggct tcggcacaca atcggtgccc ggcgcgacgc gaccttgcac atgctggccg
     1801 gcgaccggct cggcgggcgc cgcgctcctg atggacctgg agggaccacc catgaatccg
     1861 aacaccgacc cggtggacta tgcgctggcg cgccaggcgg ccggcgtgac gatcggcgcg
     1921 ctgtttgcgc gctgcgtgcg ccgcgcgccc gggcgcatcg cgctgcagca ggggccgcat
     1981 ggcctgacct acgcccagct cgacgagcgc agcaaccgcc tgtgccagca cctggcgggg
     2041 ctgggcatcg ggcgcggcga ccgggtggcg atcctgtcgg aaaaccgcac cgaatacgtc
     2101 gagctgctga tcgccgccgc caagctgggc gcgattgccg cctgccagaa ctggcgcctg
     2161 cgcgacgaag agctggccca ctgcctggcg ctggtcacgc cgcgactgat cctggcctcg
     2221 ccgcgccacg ccgaggcggc gcgccgctgc ggcggcgcgg tgccgctgct gtgcttgggc
     2281 gccgagtacg agcaggcgct gcaggccgcc accgccgagc cggtggaaga ccgctgcgac
     2341 cccgaggatg gggtggtgat cctctacacc agcggcacca ccggcatgcc caagggggcg
     2401 ctgatcagcc agcgcgccga aatcgcccgt gccgccatcc aggccatgga cctgccgcac
     2461 agcgccgacg agcacttcgt ggcctgggcg ccgctgtttc acatggtgtc caccgacacc
     2521 gtgttcatga ccctgatgca gggcggcaag gtgatcgtca ccgacggctt cgacgccgac
     2581 gagctggccg ccatcgtggc gcgcgaacgt ctggggcggc tgaccctgat gcccggcatg
     2641 atcacgccgt ttctgcaggc catgcagcgc ctgggcgcgc ggccggcggg cgtcaagtgg
     2701 gtgggcgtga tggccgacct ggtgccgcgc gagcagatcg ccgaggtcac gcggctgctg
     2761 ggcgcgccct acatcaactc cttcggcgcc accgaaaccg gctacccgcc ggcttcgctg
     2821 gccaccatcc cggtcggcgt ggtgccgacc agtctggaca agcagcagag ttcgctgtgc
     2881 cgcatccgcc tggtcgatgc cgacgaccgc gaggtgcccg atggcgagcc gggcgagctg
     2941 accatccgca cgccggcgct gttctcgggc tactggaaca acccggacgc caaccgcgag
     3001 gactttcgcg gtggctggtt ccacatgggc gacgtgtttc ggcgcaatcc ggacggcacg
     3061 ctcagcttcg tcgaccggcg caagtacctc atcaagtcgg gcggcgaaaa catctacccg
     3121 gccgaaatcg agcgcgtgct gctgtcggag ccgcgcgtgg ccgacgccgt ggtggtgcgc
     3181 cgacccgacc cgacctgggg cgaggtgccg gtggccttcg tggtgcggcg cgacccgacg
     3241 ctggacgccg ccgcgctgca ggcgctgtgc cgtcagcaga tcgccggcta caaggtgccc
     3301 aaggaagtgc acttcgtcga cgacgccgcg ctgccgcgca gcaccaccgg caagatcatg
     3361 cgtcacgagc tggagcagcg tctgccgccg gtggcgtgag ttttcagctg cgggcctgcc
     3421 accccacggc cagcagcatg aacagggcga tgaacagcgt ctcgctcacc agcagcgcga
     3481 tcggtcgcca gcccaggcgc acgatctttt cgaacgaggt cttcacgccc agcgcggcaa
     3541 tcgccaccac cagcgcccag gccgacactc cggccgccgc cgactgcagc ggcaccggga
     3601 tggcgcccag gctgttggcc accaccaggg cgatgaaggc cagcagaaag cccggcagca
     3661 gcgccggccc gcgcgggcgc ggctggccgg ccgcgtgcgc tggcgcgcga aaggccagcg
     3721 ccacgcacag caccaccggc accagcatgg cgacgcggaa catcttgacc agggtggccg
     3781 actgcgccac ctcgggcgac aggatggcgc cggcgcccac cacctgcgcc acgtcatgga
     3841 tggcgccacc cagaaacagc cccgccagcc gtgcgtccag ctgcagcgcc tgcgccacca
     3901 gcgggtacag caccatggcg gcggtggaca gcgcggccac gccgatggcg gtcatcagcg
     3961 tctgctgctc ggtgcggcca tcgcgcggca gcgcgctgga gatcgccagc gccgccgaaa
     4021 tgccgcagat gccggtggcg ccacccgaga tcaggccaaa gcgcgccggc aggccgagcg
     4081 cgcgcgccag cgccacaccg aagccgatgg tcagcgccac cgccgccgcc agccaggcca
     4141 cgccggcgcc gcccagcgcc tgcacgtcgc cgaaggcgat gcgcgcgccc agcagcgcca
     4201 cccccagccg caccagctgg cgcgcggcaa actcgatgcc ggggccgcag cgcgcgtctt
     4261 ccgacaggaa gtgcagcgcc atgcccatca gcagcgcata caggaatttg gggccgccgt
     4321 agtgttcgga cacgaaggtg gcggccagcg ccacgatcag cgccaccacc aggccgggcg
     4381 acagccggcg cagctgccgc ggccagccgc gcaccccggc cgcggcttcg gaaatttcag
     4441 acatccggcg atgctaattg tttccgtttg gcggatcgat atttctgtta ttcagaaact
     4501 gatcgaacct cactccaccg tcacgctctt ggccaggttg cgcggcttgt cgacgtcggt
     4561 gccgcgcgcc acggcggtgt ggtaggccag cagctgcagc ggcaccacgt gcaggatggg
     4621 cgacagcagg cccgagcggc ccggcatgcg gatcacgtac aggccgtcgc tgctttcgat
     4681 gtgggtgtcg gcgtcggcca gcgcgtacag cacgccaccg cgcgcgcgca cttcctgcat
     4741 gttgctcttg agcttttcca gcagcgcgtc gttgggcgcc acggtgacca ccggcatggc
     4801 gctggtcacc agcgccagcg ggccgtgctt gagctcgccg gcggcgtagg cctcggcgtg
     4861 gatgtaggtg atttccttca gcttgagcgc gccttcctgg gcgatcgggt agtgcaggcc
     4921 gcggcccagg aacagcgcgt gctccttggc ggtgaactgt tcggcccagt cgatcagctg
     4981 cggctccagc gccagcaccg cctgcagcgc gccgggcagg cggcgcagcg cgtccagctg
     5041 ctcggcctcc tgcgccgggc cgagccgagc gcgcgtcttg gccagcatca gcgccagcag
     5101 gaacaggccg accagctggg tggtgaaggc cttggtgctg gccacgccga tctcgacccc
     5161 ggcgcgcgtc agaaaggcca gccggcattc gcgcaccatg gcgctggtgg gcacgttgca
     5221 caccgtcagc gagtggcgca tgcccaggcg ctgcgcatgg cgcagcgcgg ccagggtgtc
     5281 ggcggtttcg cccgactggc tgatggtgac caccagcgtg gccggatcgg gcaccgaggc
     5341 gcggtagcgg tattcgctgg cgatttcgac ctgggtcggg atgccggcga tgtcttccag
     5401 ccagtacttg gccacgcagc cggcgtagta gctggtgccg caggccagga tcagcacctg
     5461 gcgcacggcc tgaaaggtgg tctgcgcgtc ttcgcccggc gccggcgtgc ctgccgcttc
     5521 gaacagctgg ggcaccacgg cctgcacgcc ttccagggtg tcggccacgg cgcgcggctg
     5581 ctcgaagatc tctttctgca tgtagtggcg gtagggcccc aggtcggctg cgccgctcat
     5641 ggcctgcacg gtgcgcaccg gccgttcggc gcgctggccg gcgcggtcca gcacccagta
     5701 cttgcccagc tgcacgtcga tcaggtcgcc ttcttccaga tagacgatct ggtcggtcac
     5761 gccggccagg gccatggcgt cgctggcgag gaagttttcc ttgccgtcgg cgccgatgcc
     5821 cagcaccagg ggcgagcctt cgcgcgcgcc gatcaggcgc tgcggctcgt cgcgatgcat
     5881 gacggcgatg gaatagctgc cgcgcagccg ccgcaccgcc gtctgcaccg ccgtcagcag
     5941 gtcaccgtcg tacaggctgt cgatcaggtg gacgatggtt tcggtgtcgg tctggctgct
     6001 gaagtggtag ccgcgcgcca gcagctcggc gcgcagcgct tcatggtttt cgatgatgcc
     6061 gttgtgcacc agggtgacgc ggccgggccg ctcggcgccg tcgtcggcgt gcgggcccat
     6121 gctgaagtgc gggtgcgcgt tggccaccgc cggcgcgcca tgcgtggccc agcgggtgtg
     6181 ggcgatgccg gtcaggccct gcagcccttc ggcttcgcac tgcgccgcca ggtcggccac
     6241 gcgcgtgatg ccgcgggcgc gccgcaacgc accggtcggc gcgcccaggc cggcactgtg
     6301 caccgacacg ccgcaggagt cgtagccacg gtattccagc cgctgcaggc cctgcaccag
     6361 gatgggaacg atgttgcgcg tggagaccgc gccgacgatg ccgcacatat gagcttcctc
     6421 ccaagagacg gccgtgaggg ccaggcccca cgaccgggcg atggtagccg ggcgccaaag
     6481 aaataggtgt tcattgtcga aataaatttg gaataatcat gcacccatgg gtttcaatga
     6541 aatatcaata aatcgaaagc tgtgtttatg gaattggatg acgttgactg gcgcctgctc
     6601 gacgccctgc agcacgacgc cagcctgagc aaccaggcgc tggccgagcg tctgcaactg
     6661 tcgccgccca ccgcgctgcg gcgcgtgcgc cgcctgcgcc aggccgggct gatcgccgcc
     6721 caggtggcgc tgctggacga agaccgggtg gccgccgccc agggccacgg gctgacggcg
     6781 atcgccgagg tgtcgctgga ccgccagggc gccgagcatc tggacgcctt cgagcgccgc
     6841 gcggtgcagg acaccgccgt gcagcagtgc tggcgggtgg cgcctgggcc ggacttcgtg
     6901 ctggtgctgc gcgtgcgcga catgccgcac taccagcagc tggccgagcg cctgttcacc
     6961 caggacgcca atgtgcgcaa cgtgcgggtg ttctttgcca cccggcgcgc caagttccag
     7021 acccaggtgc cgctggcacg ccccggcctg ccgaccgcgc ctcgcgttca agtaccagat
     7081 tgacctctgg cgcttaccgg gcgtgcgcag gcagctatca aaagctgagc aatgaagtag
     7141 ctgcccagca cccacagcgg ggccagcggc agtggctgca cgaagcggtt ggtggccagc
     7201 aggctgtcgc tgagcatgaa caggccggcg cccagcgcca cgcgccaggc ggcgccgtcg
     7261 cgcagccgca gggcgcggcc gacggcctgc gccaccatca gcccgatcgc cagcacatac
     7321 agggccaccg gcaggcgcag cgcggccggc agcccgccac gccaaagaaa gacgtacatg
     7381 gcggcgccga tcagggccac cgccagcgcc gccgcccggc tgggcagcca gggcaggccg
     7441 cgcagaaaca gcgccagata ggccaggtgc gccagcagaa aggccaccag gccgggcagg
     7501 aacaggcctt cgaacatcag gaacacgtcg cccgccagcg agcacgccag cgccagcatc
     7561 agaatccagc gaaatcgggt tccaacgccc gacagatcag cgcgaccagc taccaaacaa
     7621 atagcaaaca gcatcaccag cggcttgctg gcgcggtgca gcccgacggc gccggcgacg
     7681 ctggcggcgg tggccacgct ggcggccagc agcatcagta ccagccagaa ggcgtggcgg
     7741 cgggacggtg cgatgggcat gggccgccgg gccttcacca ggcgccgcgt gcgtccaccg
     7801 gcaccacggt gtccacgctg cctgcggcca gtccgccgcg caccacgatc aggtggtcgt
     7861 gcaggttgac cgtggggtcg cagtggcccg ggatcagcca cagcgtgtcg cccagcgccg
     7921 gcagcgcgcc gccgggtgcg gccgggcgca ggatgccgtg ctcgtcgcca ccgttggcgt
     7981 agtgcagcgc cggctggccg ggcagggcgt gcaccagcgg caggccgctg tcgatggcgt
     8041 ggctcttgtg gccggcgtcg cacaccacgc gctcggcgtt ggcgctgatg acctgcgcct
     8101 tgacgaacag cgccggctcg aactgcggct gcgccgggtc gcgctcgttg cgcaggtagt
     8161 cggcgtccat gaacagaaag ctgccgggct ggatctcgcc ccacagaccg ctggcggcct
     8221 cgatgccgaa gctgccggtg ccggcgccgc tgaccagcgg cggcgccagc ccggcggcgg
     8281 ccagggccgc gcaggtggtg cgcagccgct cggcggcggc ggccagcgcc tgccgccgct
     8341 cggccacacc gcgcaggtgc tgcacgccgc cgtggtaggc ctgcaggccg gcgtagcgca
     8401 ggctgctgga ggcgtcgctc agctggcgcg ccagcgccac ggcggcgtcg ccaggcggca
     8461 cgccgcagcg gccctgaccg atgtcgatct cgatgaagac gtcgatcacc gcacgcgtca
     8521 gccgcatggc ctgggccagg cgctggatgc cttcgctgct gtcgaccgcg atggccagct
     8581 ggccgccgcg cgtggccagc tggtgcgcca gcgcggccac gcggcgcagc ttgccgacgg
     8641 ccagcagctc gttggtgatg aagatgtcgt tgatgccgcc ggcggccagc gcttcggctt
     8701 cgcccaccgt ctgcacgcac aggccgacgg cgccggcctg cagctgcagg cgcgcgaact
     8761 gcgcgctctt gtgcatcttg gcgtgcgggc gcaggcgcag gcggtagcgc gtcgcgaact
     8821 cgaccatgcg ctgcaggttg cggttggcgg cgtcgagatc gaccaccagc gcgggcgtgt
     8881 cgatggcgtc caagccctgg ccgatcaggg cgcgcaggcg cggtgcgacg gggtcaactg
     8941 aagcgttcat cgaggctctc gtcttccaga tgggtggtgt cggcccagcc gaccagcgac
     9001 aggccgctgt cgggcgtcca cagcaggcgg ttgaccgcgg tgttgccgat ctgccagctg
     9061 cgcgcgtcct gcaggcccag gccggtggcc aggcgataca gcacgtcgag cacaccgcca
     9121 tgcgtgaaca gggcgatgtg attgcccatg ttttcagccg ccagcgcttg cactgcctgc
     9181 ctcacgcgct ctcgaaattg cagcaacgat tcgccgccgg cgcccggtgg cgcccaatcc
     9241 ggctcgcgtc ggcgccagcg ctcggcctgc tcgggccagc tggcttcgat ctcggcgaag
     9301 cgctggccct gcaagtcgcc gaagcagcgc tcgcgcagtg ccgtggtggt gcgcagcggc
     9361 gcgccggtgg cgtcggcgac gatgcgcgcg gtctcccgcg cgcgcgccag gtcgctgctg
     9421 acgatcagcg ccagcggctc gcgctcggcc agcgcacgcc ccagccggtg cgcctgggcg
     9481 cggccgacgt cgctcagcgc gatgtccagg tggccctgga tgcggccgtc cacattccag
     9541 gcggtttcgc catggcggac ggccaggatg cgggtggctt gcatgcgccg gatcatgcca
     9601 cgcggccggc cggtggcagg ccacgcgagc gcgtcccgca ggcgaccggt gcgcccacgc
     9661 aaaagcgttt aggcttgccg gttgcgccgc tgtgcggcct ccgcttgctt gcccatgacc
     9721 gcaccccacc gcgacgcctc cggcgcctgg accgccctgc tgctgctggc caccggcttt
     9781 ctgatgagcc aggcgtttcg caccatcgcc gccatcatgg cggcgccgct gcaggccgag
     9841 ctgcaactga cgccgcagca gctgggcacc tttgcggcgg cctaccactt cgccttcggg
     9901 ggcctgcagc tgttcatggg catgggcatc gacctgcacg ggctgcgccg cacggtgctg
     9961 acggcctcgc cgctgatggc cctgggcgcg gcggtgtcgg cgctggccac cggcttcccg
    10021 atgctggtgg cgggccaggt gctgatcggc atcggcagcg caccggcatt cctggtctgc
    10081 accatctacg tggcgcggca tttcgcgccc gagcgctaca cggtggtgtc cagcctgatc
    10141 ctggcggtgg gcggcatcgg catgctgctg accggcagcc cgctggcctg gctgatcgag
    10201 gccacgtcct ggcgcatggg cttttgggtg ctggccggct gcgcgctgct gtcgtggctg
    10261 gcgattgccc tgggcgtgcg cgagccggcg ccggcgacgg gcgcacggcg cgaaaccctg
    10321 ggccaggcgc tgcgcacctt cggcgccctg ctgcgccagc gtcacacggc cggcatcgtg
    10381 gcgctggcgc tggtgagcta tggcgccttc atcacgctgc gcgggctgtg gctgggaccg
    10441 ctgatgacgc agcgccacgg ctggagcctg gtggcggccg gccacctggc ggtgctgctg
    10501 acggtggtgt cgctggtcag cctgccgctg ttcggccgtg ccgacccggg gccgcgccgg
    10561 cgccggcgct ggatgctgtg gggcagcgcc gtcgccgcgc tgtgctttgc cggcctggcc
    10621 gccaacggcg ggatgtggct ggacgtggcg ctgatgctgg tgttcatgat gctgtcgggc
    10681 tacggcacct tgcagtaccc tgacgtcaag gacgcttacg agccggccgt gatcggccgc
    10741 gccatggcgc tgttcaccat ggcgctgttc atgggcgtgg cgctgatgca gtgggccacc
    10801 ggcgccatcg cgcaggcggc gcaggcgcgc ggcctggacc cgttcggcgc ggtgttcggc
    10861 gccatcgccg tgctgctggc gctgggcgcg ctggcgtttc gcctgctgcc gcagccggcc
    10921 agcgtgcgga cggatgtcga atgacctcgc tgcgctttga cggcaagcgc tgatcgagcc
    10981 ccgaggccgt ctcagcgccc ggtggctgcg ggggcggcgg ccggggtggc cggcggcagc
    11041 aggggcacgc cgacgcggcg ttcgctggcg gccggctgcg ggtagccttc gagcgccgcc
    11101 acgaacagcg ccgccagcac cgcgtcggtg tcgtgccagg ggccgtggtg gcgggcccgg
    11161 gtgtcgtaga cgatctggcc ggtctgcaga tcgcgcatca gcaggctgac ttcgctcgcg
    11221 taggtgggca cgctcgggtc ccccaacggc cagccccagc ccagaccgac ggcgccaccg
    11281 cgccaggcat ggcccaggcc gagcgcgatg cgggcgttgc tgggttcgta ccaggggtcg
    11341 ggccataggg cgttgacgtt gccgctgacc tgcacgctga cgcgcgcacc gccctcgtcg
    11401 cgcaccgcgc ccacgcgggt cagcgcggcc tgggccatgg cctccagccg cgccggcgcc
    11461 ggctggcccg cccgctgcgg gccaggctcg aagcggtagt gcgccccctg cagcacggcg
    11521 gcgccgggtg gctgggcggc gtgggtgcgc acatgggcgt ccaccacgcg cgggccgctg
    11581 gcgcaggcgg tcagcgcaga tgctattaaa acaagagctg ctcgcgcaat ccacatgggc
    11641 gctaacctct gatatggcgt tgaaacctca ggcgctgacc cgcgtcacgc tggaaaacgg
    11701 cgcacccacc ggcggaacaa acacctcgtc gtcgaaggcc ttgtcgccgt gctcgtcggg
    11761 cacgccgctg gggcgcaggt gcttgaagtc gtgcaggcga gcgtccatca ggtgcgaggg
    11821 caccacgttc tgcagcgcgg tgaacatgct ctcgatgcgc cccgggtggc ggcgctgcca
    11881 ttcgcgcagc atctcgccca cctgctggcg ctgcaggttt tcctggctgc cgcacaggtt
    11941 gcaggggatg atggggaact gccggtgttc ggcccaggcc accaggtcgc tttccgccac
    12001 gtaggccagc gggcggatga cgatgaattc gccgttgtcg ctgaccagct tgggcggcat
    12061 gcccttgagc ttgccgccga agaacatgtt gagaaacaag gtctgcagca tgtcgtcgcg
    12121 gtggtggccc agcgcgatct tgttgcagcc gagctggcgc gccacccggt acaggatgcc
    12181 gcggcgcagg cgcgaacaca ggctgcaggt ggtcttgccc tcggggatct tggctttgac
    12241 gatgctgtag gtgtcctgcg tttcgatatg gtgctcgatg cccaggccgg acaggtagtt
    12301 cggcagcacc tcggccggaa agccgggctg cttctgatcc aggttgacgg ccaccagatc
    12361 gaagtgaatc ggcgcgcgcg ccttgagctt gagcaggatg tcgagcaggc cgtagctgtc
    12421 cttgccgccc gacaggcaca ccatcacgcg gtcgccggcc tcgatcatgt ggaaatcggc
    12481 aatcgcctga ccgacctggc gggccaggcg tttttcgagc ttgagcgcct cgcgctcggc
    12541 gcgcgtgcgg gcggccgggc cggtgtcggc cacaacgggt tcttcgatca tgctgcgatt
    12601 gtcctacggc ggggtcggcc ctgcgcggcg ccggtgggcg gccggggctt accagtgccc
    12661 ggcctccatg tggatggaca cctcgcagtc gtcgaagatc tccagcttgg tgatcttcac
    12721 gcgcacgccc tgcacgccgg gcagctgcat caggcgcgcg cacagcttgc cgatcagcgt
    12781 ctccagcagg ttgacgtgct cggcggtgca ctcgtcgatg atgatctggc gcaccttgcg
    12841 gtagtccagc acatgggtga tgtcgtcgtc gcgcgggcgc agcggctgcg cgcccaggtg
    12901 cagctcggca tcgacctgga tcggctgcgg cgccagcttt tcgtgcgcca ggatgcccag
    12961 gttggcggca aagcgcaggc cggtgagttg caggctctgc tggccctgtt cggcgcgggg
    13021 gtggctgggc atggggcggt tcaccagggt ttgacggcca cgcgctcgac gccgaccgag
    13081 gcgctgtcgg ggtagacgtc gggcttgcgg gtggccacgc gcgcggcctg cacctcgggg
    13141 cgtgccagca cggcggccag cacggcgtcg cacagggttt cctgcagctc gtggtgggcc
    13201 tgaccgacgg cggcggcgat gacgtcgcgc acgaagtcgt agtccagcgt gtgggcgatg
    13261 tggtcgtgcg cggcggcggc gtcgcgcagg cgcacgcaca gatcgacgtc gaaccacagg
    13321 cgctgcggtc cctggcgctc gaactcgtgc acaccgatgc gcaccggcag ttcgtggcgg
    13381 cgcaggctca gcaggcggca gtccagcgcc aggcgcgcgt ccagcgcgcc gaaggccagc
    13441 agcagggaat tcatcgggtc agcccttcgt cgacggcaaa catcacgtcg cgcgccagcg
    13501 gcaccaggtg ctgaccgttg tcggcctgca gggtggtgcc gctcagggcc ggggtttcgg
    13561 caatgaacac gacgcagcgc gccacctgcg ccggatcgag cggctcgcgc agcaggttga
    13621 cgcggctggc gcgctcgaag ttgtcctgcg tctgcgggcc gctcaggtac atcagccccg
    13681 gcgacagccc gcacacccgc acccagggcg ccagcgcctg cgcctgcagg gccacgctct
    13741 gcgccagcgc cagcttg
//
LOCUS       DAOQTZ010000076        13601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5109_length_13601_cov_13.912373, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000076 DAOQTZ010000000
VERSION     DAOQTZ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13601
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5109_length_13601_cov_13.912373"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..887)
                     /locus_tag="PKN51_09610"
     CDS             complement(60..887)
                     /locus_tag="PKN51_09610"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNT85395.1"
                     /translation="MSTPTPTRMPTLYIPHGAGPCFFMDWNPPDTWARTAAFLRGIPD
                     SLPAAPTAILLVTAHWLAPQPSLSSAAQPGLLFDYHGFPPHTYELSYPAPGAPALARR
                     AAALLTDAGFAPRLDDARGYDHGSFIPLKLAFPAADVPVLQMSLLRSLDAGEHLRLGQ
                     ALAPLRDEGVLIVGSGMSYHHMRGYGDPRSTPVAAEFDRWLAETVALPAAERHARLTD
                     WARASGPAGRLSHPAQAEEHLLPLHVVAGAAGDSRGRRVFTDRVMAVDISAFRFDDS"
     gene            1040..1468
                     /locus_tag="PKN51_09615"
     CDS             1040..1468
                     /locus_tag="PKN51_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT85396.1"
                     /translation="MTEPTACPEPPWRHILRDVPDALIYADAEGVIRAWNAGAEALFG
                     FSAAEALGASLDLIIPEWLREPHWVAYRRAMARGATRGGAEVRTTRGTHRDGRKLYVD
                     MSFGVVRDDHGQVLGSVAMARDATARHLAEMARRTATGGT"
     gene            1529..2530
                     /gene="rssA"
                     /locus_tag="PKN51_09620"
     CDS             1529..2530
                     /gene="rssA"
                     /locus_tag="PKN51_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HNT85397.1"
                     /translation="MTHPPPAHAAFARRAPVIGLALGSGSARGWAHFGVLRALTDCGI
                     RPGVVCGASIGALVGAAHVLGLTERFERWVLGLRRRDVVSFMDISTGGGLLKGEKLMR
                     FFRSQFTDRDFDALETPFAAVATALHTGEEVWLRQGSVVDAVRASIAMPGLFTPVWHQ
                     GRLLVDGGLVNPVPVSLARALGADIVIAVDLNADLIGRHLRHGPAQAAVPASEPSIGW
                     LQNLQRSLGWHRDEAAAQDATPPIPPVLEVLTSGIHIMQVRITRSRMSGDPPEFTIAP
                     RLSHLSLLDFHRAHEAIEAGEQAAQESLGLLRELGLCPPTRETTPAAPPDAASGATG"
     gene            complement(2556..3662)
                     /locus_tag="PKN51_09625"
     CDS             complement(2556..3662)
                     /locus_tag="PKN51_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNT85398.1"
                     /translation="MIPIPQAFAASYAQARVKFLEAAASASTQIESHRHPLPGRDGEL
                     LALDLALDGDAQSERLLIVSSACHGVEGFCGSGVQVFALHDADWRQQARDAGVAVLYL
                     HALNPWGFSHLRRVTHEGVDLNRNFPDFSQPLPVNTAYRQLHPLLLPSQWPPDAAHRA
                     AVQDFIAREGVAAWQTAISGGQYEFPTGLFYGGHAPTWSHQTLRGVLARHAGRARRVA
                     WIDLHTGLGPPGVGERIWAGGQNAAGVARARRWWGEQVTSIHDGSSTSPPLTGLMWSA
                     IDAAAPQAEYTGIALEFGTEPILEILDALRGDHWLAQHPEAPPALAADIKARLRRAFY
                     TDTDAWKGQIVSQARQALMQAVQGLSEGGRGTAA"
     gene            complement(3835..5079)
                     /locus_tag="PKN51_09630"
     CDS             complement(3835..5079)
                     /locus_tag="PKN51_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HNT85399.1"
                     /translation="MKLLEPILAQAAAISQLRRDIHAHPELCFEEVRTADLVAAKLAE
                     WGIATHRGMGTTGVVGIVHGRDGGKSGRAIGLRADMDALPMTEFNTFAHASRHHGKMH
                     ACGHDGHVAMLLAAGQQLAATRDFDGTVYLIFQPAEEGGGGAREMVKDGLFDRFPMEA
                     VFGMHNWPGMEAGRFAVSAGPVMGSSNEFRIVITGKGGHAAMPHTGIDPLPIACQVVL
                     GFQTIISRHKKPVDPGVISVTMVHAGEATNVVPDACEIQGTVRTFSFETLDLIEQRMQ
                     AIAEGVSAAFGAQCTFEFTRNYPPTINTAYEAEFCRRVMAGIVGEANAVPQEPTLGAE
                     DFAYMLLEKPGAYCFIGNGMGAHRGRYEGGGHDAGPCTLHNPHYDFNDDLIPLGGTYW
                     VQLAQQWLAHQPPADRPLPRKG"
     gene            complement(5263..5604)
                     /locus_tag="PKN51_09635"
     CDS             complement(5263..5604)
                     /locus_tag="PKN51_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HNT85400.1"
                     /translation="MAGKFELKKSKNGKFFFNLLASNGQVILSSEMYEAKASAVNGIE
                     SVKKNGADAAHYEKLTGKDGSPYFTLKAGNGQVIGQSQMYASETTRDNGIQSCVSHAP
                     GATVDDQTAQA"
     gene            complement(5738..6895)
                     /gene="rodA"
                     /locus_tag="PKN51_09640"
     CDS             complement(5738..6895)
                     /gene="rodA"
                     /locus_tag="PKN51_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNT85401.1"
                     /translation="MAVVFDKPSLWQRLWPLLRGFDLLLLVAVLVLAGMGLMTMYSSG
                     FDHGTRFADHARNMLIAAGIMFVVAQVPPQRLMSLAVPVYTLGVGLLVAVALFGITKK
                     GAQRWINVGVVIQPSELLKIATPLMLAWWFHRREGQTRPVDFLVAFALLAAPVGLIMK
                     QPDLGTALLVGAAGLAVIFFAGLPWKLVLPPLLLAALGVGLLLAYEGPLCADGVDWRV
                     LHEYQRQRVCTLLDPTRDPLGKGFHTLQGMIAIGSGGLWGKGFMQGTQTHLEFIPERT
                     TDFIFAAFSEEFGLAGNLALIAGFVFLILRGLMIAAEGPTLFARLLAAALTTIFFAYA
                     FVNMGMVSGMLPVVGVPLPFISYGGTAMVTLGLALGMLMSIAKARRTLMQS"
     gene            6990..7574
                     /locus_tag="PKN51_09645"
     CDS             6990..7574
                     /locus_tag="PKN51_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HNT85402.1"
                     /translation="MPTTHLLYLHGFRSSPQSAKARQMAARVRADHPGVTWWCPQLPP
                     SPREAAALIAQGSADWPAAHMAVVGSSLGGYYATWLAQRRGCRAVVLNPAVDPARDLA
                     AHIGEQTAWHDPAERFLFRPEYVDELRALAPGPLQAPERFLAVIAQGDELLDWREMAA
                     RYAGAALRLIEGSDHALSDFEHHIDAVLSFLDLL"
     gene            7712..8239
                     /locus_tag="PKN51_09650"
     CDS             7712..8239
                     /locus_tag="PKN51_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNT85403.1"
                     /translation="MTPTPTPVRYDRISRWLHWGMAGLFAWIFVSAGAHALAERSALD
                     ALLWPTHKPVGTLLLALLLARVAWALARRRRPPAVSALARLGHLGLYALMLAVPAIGL
                     LRQWGSGRASAPFGLPLLPGFDVSQKVEWTLQLGRLLHGNLGWALLALVLGHVAAALW
                     HERAGHTPVLARMAG"
     gene            8277..10346
                     /locus_tag="PKN51_09655"
     CDS             8277..10346
                     /locus_tag="PKN51_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNT85404.1"
                     /translation="MHVLFDEAGKFLAGRILSEAEASAQVELDSGKRVKVKAANQLLR
                     FDKPAPAELLAQAQAQAADVEPALAWEFAPEDEFGFAELAADYFGGGPGPVQQAAALI
                     ALHEAPHYFRRAGKGRFRKAPADVVAQALAAIEKKKQLAAQIAAWADALAQGQCPAPV
                     REQLYRILFRPDRNAPEYKAVVEASRATQLAPLALLQRAGAIESAYQFHWQRFLFENF
                     PQGTGFARLAAPVIREELPLAPVAAFSIDDSATTEIDDALSLQGLGTGTVVLGIHIAA
                     PGLAIQPGDALDDVARARLSTVYMPGYKITMLPDEVVDAYTLAEGAPVPAVSLYVTLD
                     EATLEIQDRQTRLERVPIAANLRHDQLDALITEDWLQGASVDGQIDPQRLPVEREQLS
                     FLYQLSRHLKARREQVRGKPETFTRPDYSFRLVGNDGAVPTGAEQVQITTRSRGAPLD
                     LIVAELMILANSSWGEWLKALGVPAIYRSQASLLPGIKVRMGARALPHAGLGVPSYAW
                     STSPLRRYVDLVNQWQIIAAARHGTAAALVAPFKPKDAALLAIISAFDAAYSAYGAHQ
                     ASMERFWTLRYLHQQGITELNASLLREQTGGAWLVRADELPLVFTVMGAQGLPRGTRV
                     RVRLGQLDDIALDVHGTVLEVLQDQAAVPTAAEAEDDDEGAAAGPIAIAVELDETEGA
                     RTDNPPS"
     gene            10346..11242
                     /locus_tag="PKN51_09660"
     CDS             10346..11242
                     /locus_tag="PKN51_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNT85405.1"
                     /translation="MLRSYKTLSTLQWALGLSVLLHAVLLTVRFVDPEGFKRLFQETT
                     LDVVLVNAASDEKPVKAQAIAQSSLAGGGEAADRRMASTPLPPSPHEAPGDAPVTENQ
                     RRIDAMLQQQEQLLAQVRQQLAAMPKPDPRQLSEDPEARAQEERRQQLARLLAAIEKR
                     VEEENSRPRKRYLSPATLGTTYAVYYDSMRRKIEAEGTANFPQVAGRKLYGELIMALL
                     INHDGRILDARVVRGSGQRALDKLAELIAARAAPFGNFTAAMRKDTDQFDVTARFRFT
                     REQTLETMLQADPLATSVRELK"
     gene            11307..12035
                     /gene="mtgA"
                     /locus_tag="PKN51_09665"
     CDS             11307..12035
                     /gene="mtgA"
                     /locus_tag="PKN51_09665"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNT85406.1"
                     /translation="MGAVLRRILLLAVLGLLLLQLFFVGRVALMVVLDPRSTSFERSE
                     AWRLASTGTLRWRQQWRDYAQISDHLKRAVIASEDDGFANHEGVDWDAIEKAWQRNAA
                     AEEAAAERAAQNRPARPIRIRGGSTITQQLAKNLLLSGERSLWRKGQELVLTFALEQL
                     LDKRRILELYLNHVEWGEGVFGAEAAAQHYFRKSAAQLTAAESARLAVMLPRPRYFEK
                     LPRSAYLASRTRTILARMRSAELP"
     gene            complement(12171..12626)
                     /locus_tag="PKN51_09670"
     CDS             complement(12171..12626)
                     /locus_tag="PKN51_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HNT85407.1"
                     /translation="MATAKKAPAKKAPAKKAAPAKKAPAKKAAPAKKAAPAKKAAPAK
                     KAAPAKKAPAKKAAPAKKAAPAKKAAPAKKVAAKKAAPAKKAAPAKKAAPAKKVAAKK
                     AAPAKKAAPAKKPAAKKAAPAKKAAAKKPAAQTTLSPQAAWPFPTASKP"
     gene            complement(12809..>13601)
                     /locus_tag="PKN51_09675"
     CDS             complement(12809..>13601)
                     /locus_tag="PKN51_09675"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNT85408.1"
                     /translation="VTADSLAANNIVLGTYRHVTAPECRQFLLRQAFEEAIHTHAYQY
                     IVESLGLDEGEIFNAYHEIPSIRDKDEFLIPFIDAIMDPDFRTGTPEADQRLLKSLIV
                     FACLMEGLFFYVGFTQILALGRQNKMVGAAEQYQYILRDESMHCNFGIDLINQIKLEN
                     PHLWTPAFKAEVRALFEHAVELEYRYAEDTMPRGVLGMNASMFKGYLRHIANRRATQI
                     GLDALYPNAENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD"
BASE COUNT         1974 a         4687 c         4865 g         2075 t
ORIGIN      
        1 gaccccgcat cgatgcggcc cgcccgaagt tgacaagcgg tcaactgccg tttctgggtt
       61 caggaatcat cgaagcggaa cgccgagatg tccaccgcca tcacgcggtc ggtgaacacc
      121 cggcggccgc ggctgtcgcc ggccgcgcca gctaccacgt gcagcggcag caggtgctct
      181 tcggcctgcg ccgggtggct cagacgcccg gcggggccgc tggcgcgcgc ccagtcggtc
      241 agtcgggcgt ggcgctcggc cgccggcagg gccaccgtct cagccagcca gcgatcgaac
      301 tcggccgcca ccggggtcga gcgcggatcg ccatagccgc gcatgtggtg gtagctcatg
      361 ccgctgccga cgatcagcac gccctcgtcg cgcagcggcg ccagcgcctg acccagccgc
      421 aggtgctcgc ccgcgtccag gctgcgcagc agcgacatct gcagcaccgg cacgtcggcg
      481 gccggaaagg cgagcttgag cggaatgaag ctgccatggt catagccgcg cgcatcgtcc
      541 agccgcggcg cgaagccggc atcggtcagc agggcggcgg cgcgccgggc cagcgccggt
      601 gcaccagggg cggggtagct cagttcgtag gtgtggggcg gaaagccgtg gtaatcgaac
      661 agcaggccgg gctgcgcggc gctgctcagg ctgggctggg gcgccagcca gtgcgccgtc
      721 accagcagga tggcggtcgg cgcggccggc aggctgtccg gaatgccgcg caggaaggcg
      781 gcggtgcgcg cccaggtgtc gggcgggttc cagtccatga agaagcaggg tccggcgccg
      841 tgcgggatgt acagcgtcgg catgcgggtc ggcgtgggcg tggacatggt gaggtggccg
      901 cgggtgggcg atggcgcgat tgtccggcag cgcgcgcgca ggcgggtgcc accggccgca
      961 agcccctgcc cttgccacgg gcgccgcctg ggggcaggat gcgggcctgc cttcttcgtc
     1021 cgcctgcgcc gttgccgcca tgaccgaacc gaccgcctgc cccgaaccgc cctggcgcca
     1081 catcctgcgc gacgtgcccg atgcgctgat ctatgccgat gccgaaggcg tgatccgcgc
     1141 ctggaatgcg ggtgccgagg cgctgtttgg cttcagcgcc gccgaagcgc tgggcgccag
     1201 cctggacctc atcattccgg aatggctgcg cgagccgcac tgggtggcgt accggcgcgc
     1261 catggcgcgc ggcgccaccc gcggcggcgc cgaggtgcgt accacgcgcg gcacgcaccg
     1321 cgacggccgc aagctgtacg tcgacatgag ctttggcgtg gtcagggacg accacggcca
     1381 ggtgctgggt tcggtggcca tggcgcgcga cgccacggcg cgccatctgg ccgagatggc
     1441 acggcgcacc gccaccggcg gcacctgagc ggcagccgca cggccccgcc cgtgcgacac
     1501 tgtccctgtc ccacgcagcc cggcacgcat gacccacccg ccacccgctc acgccgcctt
     1561 tgcccgacgc gcgcccgtca tcggtctggc gctgggcagc ggttcggcgc gcggctgggc
     1621 acatttcggt gtgctgcgtg cgctgacgga ctgcggcatc cgtcccggcg tggtgtgcgg
     1681 cgcctcgatc ggggcgctgg tgggcgcggc ccatgtgctg gggctgaccg agcgcttcga
     1741 gcgctgggtg ctgggcctgc gccggcgcga tgtggtgtcg ttcatggaca tcagcaccgg
     1801 cggtggcctg ctcaagggcg agaagctgat gcgatttttc cgcagccagt tcaccgaccg
     1861 tgacttcgat gccctggaga cgccgtttgc cgccgtcgcc accgcgctgc acaccggcga
     1921 ggaagtctgg ctgcgccagg gctcggtggt ggatgccgtg cgcgcctcga tcgccatgcc
     1981 gggcctgttc acgccggtgt ggcaccaggg gcgcctgctg gtcgatggcg ggctggtcaa
     2041 tccggtgccg gtgtcactgg cgcgcgcgct gggcgccgac atcgtgatcg cggtcgatct
     2101 gaacgccgac ctgatcgggc gccacctgcg ccacggcccg gcccaagccg ccgtgccggc
     2161 cagcgagccg tcgatcggct ggctgcagaa cctgcagcgc agtctgggct ggcaccgcga
     2221 cgaggccgcc gcccaggatg cgacaccgcc gatcccgccg gtgctggagg tgctgaccag
     2281 cggcatccac atcatgcagg tgcgcatcac gcgcagccgc atgagtggtg acccgccgga
     2341 attcaccatc gcgccgcgcc tgtcgcacct gtcgctgctg gatttccatc gtgcgcacga
     2401 ggccatcgag gcgggcgagc aggcggcgca ggaaagcctg ggcctgctgc gtgaactggg
     2461 cctgtgtccg ccgacgcgcg aaaccacgcc cgctgccccg cccgatgcgg catcgggggc
     2521 caccggatga tccggcgcgg tggcagggca aggcctcagg ccgccgtgcc gcggccgcct
     2581 tcgctcagcc cctgcaccgc ctgcatcagc gcctggcgcg cctggctgac gatctggccc
     2641 ttccaggcgt cggtgtcggt gtagaaggca cgacgcaggc gcgccttgat atcggccgcc
     2701 agcgccggcg gcgcctcggg gtgctgggcc agccagtggt cgccgcgcag ggcgtcgaga
     2761 atctcgagga tcggctcggt gccgaattcg agcgcgatgc cggtgtattc ggcctgcggc
     2821 gcggcggcgt cgatggccga ccacatcagg ccggtgagcg gtgggctggt ggaggagccg
     2881 tcgtggatcg atgtcacctg ctcgccccac cagcggcgcg cgcgcgccac gccggcggcg
     2941 ttctggccgc cggcccagat gcgctcgccc acgcccggcg ggcccaggcc ggtgtgcagg
     3001 tcgatccagg ccacgcggcg cgcgcgtccg gcgtgccgcg ccagcacgcc gcgcagggtc
     3061 tggtggctcc aggtcggcgc gtggccgccg tagaacaggc cggtggggaa ttcgtactgg
     3121 ccgcccgaga tggcggtctg ccaggcggcc acgccctcgc gcgcgatgaa gtcctgcacc
     3181 gcggcgcggt gggcggcgtc gggcggccac tgggacggca gcagcaacgg atgcagctgg
     3241 cgataggcgg tgttgaccgg cagcggctgg ctgaagtcgg ggaagttgcg gttcaggtcc
     3301 accccttcgt gcgtcacgcg ccgcaggtgc gagaaacccc aggggttgag cgcatgcagg
     3361 tagagcaccg ccacgccggc gtcgcgcgcc tgctggcgcc agtcggcgtc gtgcagggca
     3421 aacacctgca cccccgagcc gcaaaagccc tcgaccccgt ggcaggcgct gctgacgatg
     3481 agcaggcgct cactctgcgc gtcaccatcg agcgccaggt ccagcgccag caactcgccg
     3541 tcgcggccgg gcaggggatg gcggtggctc tcgatctgcg tgctggcgct ggccgccgct
     3601 tcgaggaact tgacgcgcgc ctgggcgtag ctggcggcaa aggcctgcgg gatcgggatc
     3661 atggcggcag gctcagcagc tgggcatgca tgtcgggcac gccaggggta cgccgcgacg
     3721 cggccgatca gggcgccgcg cggcgcctgc gcctgcgcca cggcggccac gggccacgtc
     3781 gggcggctgc gttgcggcgg tcgcagcgga catgaaaggt tcgctcacac cggctcagcc
     3841 cttgcgcggc agcgggcggt cggccggggg ctggtgcgcc agccactgct gcgccagctg
     3901 cacccagtag gtgccgccca gcgggatcag atcgtcgttg aagtcgtagt gcgggttgtg
     3961 cagggtgcag gggccggcgt catggccacc gccttcgtag cgcccgcggt gcgcgcccat
     4021 gccgttgccg atgaagcagt aggcgccggg tttttccagc agcatgtagg cgaagtcctc
     4081 ggcgcccagg gtgggttcct gcggtacggc gttggcttcg ccgacgatgc cggccatcac
     4141 gcggcggcag aactcggcct cgtaggcggt gttgatggtg ggcgggtagt tgcgcgtgaa
     4201 ttcgaacgtg cactgggcgc cgaaggcggc gctcacccct tcggcgatgg cctgcatgcg
     4261 ctgctcgatc agatcgagtg tctcgaagct gaaggtgcgc accgtgccct ggatctcgca
     4321 cgcgtcgggc accacgttgg tggcctcgcc ggcgtggacc atggtgaccg agatgacgcc
     4381 cggatccacc ggcttcttgt ggcgtgagat gatggtctga aaacccagca ccacctggca
     4441 ggcgatcggc agcgggtcga tgccggtgtg gggcatggcc gcgtggccgc ccttgccggt
     4501 gatgacgatg cggaattcgt tgctggagcc catcaccggg ccggccgaga ccgcaaacct
     4561 gccggcttcc atgcccggcc agttgtgcat gccgaacacc gcttccatcg ggaagcggtc
     4621 gaacaggccg tccttgacca tttcgcgcgc gccgccgccg ccttcttcgg cgggctggaa
     4681 gatcaggtac accgtgccat cgaagtcgcg cgtggccgcc agctgctgac cggcggccag
     4741 cagcatggcc acatggccgt cgtggccgca ggcatgcatc ttgccgtggt ggcggctggc
     4801 gtgggcgaag gtgttgaatt cggtcatcgg cagcgcgtcc atgtcggcac gcaggccgat
     4861 ggcgcggccc gacttgccgc cgtcacgccc gtgcacgatg ccgaccacac cggtggtgcc
     4921 catgccgcgg tgggtggcaa taccccactc ggccagcttg gccgccacca ggtcggcggt
     4981 gcgcacctct tcgaagcaca gctcggggtg ggcgtggatg tcgcgccgca gttgggagat
     5041 ggccgcggcc tgcgccagga tgggttcgag cagtttcatg gccccagtct agccgttggc
     5101 gcagggcctt gcatatccgt cagaacgcta tgattacaat agctgctggc gcttggtggg
     5161 tgcgccgtag cggcaaaaaa caccactgaa aacaaggccg ccgcggcgtg ccggacgcaa
     5221 aaaagccgcc cgcaggcggc tggctggctg gggctgaccc ggtcaggcct gggcggtctg
     5281 gtcgtcgacc gtggcgccgg gggcgtggct gacgcaggac tggatgccgt tgtcgcgcgt
     5341 cgtctcgctg gcatacatct ggctctggcc gatcacctgg ccgttgccgg ccttgagcgt
     5401 gaagtagggc gagccgtcct tgccggtcag tttttcgtag tgcgcggcgt cggcgccgtt
     5461 tttcttcacc gattcgatgc cgttgaccgc gctggccttg gcctcgtaca tctcgctgct
     5521 gaggatgacc tgaccattgc tggccagcag gttgaagaag aacttgccgt tcttggactt
     5581 cttgagttcg aacttgcctg ccatgaatgt cgctccttga gtgatggggg gtggatgcgc
     5641 cgggcccagc ccggctgccc ggcattgtgg ggccaagagg ggccgtccgg gcgacttcgg
     5701 ggcacgccgg cgtgcaatgc cgcacaccga cgtggcgtca actctgcatc agggtgcgcc
     5761 gcgccttggc gatcgacatc agcatgccca gcgccagccc gagcgtgacc atggccgtgc
     5821 cgccgtagct gatgaagggc agcggcacgc ccaccaccgg cagcatgccg ctgaccatgc
     5881 ccatgttgac gaaggcgtag gcaaagaaga tggtggtcag cgccgccgcc agcaggcggg
     5941 cgaacagcgt cggcccctcg gcggcgatca tcagcccgcg caggatcaga aacacgaagc
     6001 cggcgatcag cgccaggttg ccagccaggc cgaattcctc cgagaaggcg gcgaagatga
     6061 agtcggtggt gcgctcggga atgaactcca ggtgcgtctg cgtgccctgc atgaagccct
     6121 tgccccacag cccgcccgag ccgatggcga tcatgccctg cagcgtgtga aagcccttgc
     6181 ccagggggtc gcgcgtgggg tcgagcaggg tacacacgcg ctggcgctgg tattcgtgca
     6241 gcacgcgcca gtccacgccg tcggcacaca gcgggccttc gtaggccagc agcaggccca
     6301 cgcccagcgc cgccagcagc agcggcggca acaccagctt ccagggcagg ccggcaaaga
     6361 agatcaccgc cagcccggcg gcgcccacca gcagggcggt gcccaggtcg ggctgcttca
     6421 tgatcagccc cacgggcgcg gccagcagcg cgaacgccac cagaaagtcg actgggcgcg
     6481 tctggccctc gcggcggtga aaccaccagg ccagcatcag cggggtggcg atcttcagca
     6541 gctcgctggg ctggatcacc acgccgacgt tgatccagcg ctgcgccccc ttcttggtga
     6601 tgccgaacag cgccaccgcc accagcaggc ccacgcccag ggtgtagacc ggcaccgcca
     6661 ggctcatcag ccgctgcggc ggcacctgcg ccaccacgaa catgatgccg gcggcgatca
     6721 gcatgttgcg ggcgtggtcg gcaaagcgcg taccgtggtc gaagccggag gaatacatgg
     6781 tcatcagacc catgccggcc agcaccagca ccgccaccag cagcagcagg tcgaagccgc
     6841 gcagcagcgg ccacagacgc tgccagagcg acggtttgtc gaacaccacg gccatggcgc
     6901 cattatcccg ccgccccgca gcgcagccct gcggcgccgg tatggattgg gttcaaatgt
     6961 caggcttccg aagccatagc attgcccgca tgccgaccac ccatttgctg tacctgcacg
     7021 gatttcgctc ctcgccgcaa tcggccaagg cgcgccagat ggcggcgcgc gtgcgcgccg
     7081 accacccggg cgtgacctgg tggtgcccgc agctgccgcc ctcgccccgc gaggccgcgg
     7141 cgttgatcgc gcagggcagc gccgactggc cggccgcgca catggcggtg gtgggctcct
     7201 cgctgggcgg ttactacgcc acctggctgg cccagcggcg cggctgccgg gcggtggtgc
     7261 tcaacccggc ggtcgatccg gcgcgcgacc tggcggcgca catcggcgag cagaccgcct
     7321 ggcacgaccc ggccgagcgc ttcttgttcc gccccgaata cgtggacgag ctgcgcgccc
     7381 tggcacccgg cccgctgcag gcgcccgagc gctttctggc cgtcatcgcc cagggcgacg
     7441 agctgctgga ctggcgcgag atggcggcgc gctacgccgg cgcggcgctg cggctgatcg
     7501 agggcagcga ccacgcgctg agcgacttcg agcaccacat cgacgccgtg ctgagctttc
     7561 tggaccttct gtaacgctat cgtaactata gcttccggcg cagtgtgggc atgtgccaaa
     7621 ggcctttaca accgcttgac acggcagacg gacgggcttc accggcgggt gacagcatca
     7681 ccggcacccg aaccccggaa aggcttgcac gatgaccccc acccccaccc ccgtccgcta
     7741 cgaccgcatc agccgctggc tgcactgggg catggccggg ctgttcgcct ggatcttcgt
     7801 ctccgccggc gcgcatgcgc tggccgagcg ctcggcgctg gacgcgctgc tgtggcccac
     7861 ccacaaaccg gtcggcaccc tgctgctggc cctgctgctg gcgcgtgtcg cctgggcact
     7921 ggcgcggcgc cgccggccgc cggcggtgag cgcgctggcc cgtctggggc acctgggcct
     7981 gtacgccctg atgctggccg tgccggccat cgggctgctg cgccagtggg gctcgggacg
     8041 cgcgtctgcg ccgttcggcc tgccgctgct gccgggcttc gacgtcagcc agaaggtgga
     8101 atggacgctg cagctgggcc ggctgctgca cggcaacctg ggttgggcct tgctggccct
     8161 ggtgctgggc cacgtggcgg cggcgctgtg gcacgagcgc gccggccaca cgcccgtgct
     8221 ggcgcgcatg gccggctgag cgccggcgcg gcagggagtg ggacaatcgg cgtcccatgc
     8281 atgtactgtt cgacgaggcc ggcaagtttc ttgccggccg cattctttcc gaggccgagg
     8341 cctcggccca ggtggagctg gactccggca agcgcgtcaa ggtcaaggcc gccaaccagt
     8401 tgctgcgctt cgacaagccg gcgccggccg agctgctggc ccaggcgcag gcccaggccg
     8461 ccgacgtcga accggccctg gcctgggaat tcgcgccgga agacgagttc gggtttgccg
     8521 agctggcggc cgactacttc ggcggcggcc ccggcccggt gcagcaggcg gcggcgctga
     8581 tcgcgctgca cgaggcgccg cactacttcc gccgcgccgg caaggggcgc tttcgcaagg
     8641 cgccggccga cgtcgtggcg caggcgctgg cggcgatcga gaagaaaaag cagctcgcgg
     8701 cccagatcgc cgcctgggcc gacgcgctgg cgcagggcca gtgcccggcg ccggtgcgcg
     8761 agcagctcta ccgcatcctg ttccgcccgg accgcaacgc ccccgaatac aaggccgtgg
     8821 tcgaggccag ccgcgccacc cagctggcgc cgctggcgct gctgcagcgc gccggcgcca
     8881 tcgagtcggc ctaccagttc cactggcagc gctttctgtt cgagaacttc ccgcagggca
     8941 cgggttttgc ccggctggcc gcgccggtca tccgcgagga gctgccgctg gcgccggtgg
     9001 cggcgttttc gatcgacgac tcggccacca ccgaaatcga cgacgcgctg tcgctgcagg
     9061 ggctgggcac cggcacggtg gtgctgggca tccacatcgc ggcgcccggc ctggccattc
     9121 agccgggcga cgcgctggac gacgtggcgc gcgcgcgcct gtccaccgtc tacatgccgg
     9181 gctacaagat caccatgctg cccgacgagg tggtcgacgc ctacacgctc gccgaaggcg
     9241 cgccggtgcc ggcggtgtcg ctgtatgtca cgctggacga agccacgctg gagatccagg
     9301 accgccagac gcggctggag cgggtgccga tcgccgccaa cctgcgccac gaccagctcg
     9361 acgcgctgat caccgaggac tggctgcaag gcgcttcggt tgatggtcaa atcgaccccc
     9421 agcgcttgcc tgtcgagcgc gaacagctat catttttata tcaactgagc cggcacctga
     9481 aggcccggcg cgagcaggtg cgcggcaagc cggaaacctt cacgcggccg gactacagct
     9541 tccgcctggt cggcaacgac ggcgccgtgc ccaccggcgc cgagcaggtg cagatcacca
     9601 cccgctcgcg cggcgcgccg ctggatctga tcgtcgccga actcatgatc ctggccaaca
     9661 gcagctgggg cgaatggctc aaggccctgg gcgtgccggc catctaccgc agccaggcca
     9721 gcctgctgcc cggcatcaag gtgcgcatgg gcgcgcgggc gctgccgcac gccggcctgg
     9781 gtgtgcccag ctacgcctgg agcacctcgc cgctgcgccg ctacgtcgac ctggtcaacc
     9841 agtggcagat catcgccgcc gcacgccacg gcacggcggc ggcgctggtg gcgccgttca
     9901 agcccaagga cgcggcgctg ctggccatca tcagcgcctt cgatgccgcc tacagcgcct
     9961 atggcgcgca ccaggccagc atggaacgct tctggacgct gcgctacctg caccagcaag
    10021 gcatcaccga gctgaacgcc agcctgctgc gcgagcagac cggcggcgcc tggctggtgc
    10081 gcgccgacga gctgccgctg gtgtttaccg tgatgggcgc gcagggcctg ccgcgtggca
    10141 ctcgggtgcg ggtgcggctg ggccagctcg acgacatcgc gctcgacgtg cacggcaccg
    10201 tcctcgaggt gttgcaggac caggcggcgg tgcccacagc cgccgaggcc gaggacgacg
    10261 acgagggcgc cgccgccggg ccgatcgcca ttgcggtgga gctggacgaa accgaaggcg
    10321 cgcgcaccga taatccgccc tcgtaatgct tcgttcgtat aaaacgctgt ccacgctgca
    10381 atgggcgctc ggcctgtcgg tgctgctgca cgcggtgctg ctgacggtgc gtttcgtcga
    10441 tcccgagggc ttcaagcgcc tgtttcagga gaccacgctc gacgtggtgc tggtcaacgc
    10501 cgcatcggac gagaagcccg tcaaggccca ggccatcgcc cagtccagcc tggccggtgg
    10561 cggcgaagcg gccgatcggc gcatggccag cacgccgctg ccgcccagcc cccacgaagc
    10621 gccgggcgat gcgccggtca ccgagaacca gcgccgcatc gacgccatgc tgcagcagca
    10681 ggagcagctg ctggcccagg tgcgccagca actggcggcc atgcccaagc ccgacccgcg
    10741 ccagctgagc gaagatcccg aggcgcgcgc ccaggaagag cgtcgccagc aactggcgcg
    10801 cctgctggcg gcgatcgaaa agcgggtcga ggaagagaac tcgcgcccgc gcaagcgcta
    10861 cctcagcccc gccacgctgg gcaccaccta cgcggtctac tacgacagca tgcgccgcaa
    10921 gatcgaggcc gagggcaccg ccaactttcc ccaggtggcc gggcgcaagc tgtatggcga
    10981 gctgatcatg gcgctgctga tcaaccacga cggccgcatt ctcgacgccc gggtggtgcg
    11041 cggctcgggc cagcgtgcgc tggacaagct ggccgagctg atcgcggcgc gcgccgcccc
    11101 gttcggcaac ttcaccgccg ccatgcgcaa ggacaccgac cagttcgacg tcaccgcgcg
    11161 cttcaggttc acgcgcgagc agaccctgga gaccatgctg caggccgacc cgctggcgac
    11221 ctcggtgcgg gagttgaaat gaaccacccg cgccccgcca cccaccgacc gccgggcgtg
    11281 gtctgacggc gggacgggca tcgaccatgg gggctgtgct gcgccgcatc ctgctgctgg
    11341 ccgtgctggg cctgctgctg ttgcagctgt tcttcgtcgg ccgcgtggcg ctgatggtgg
    11401 tgctcgaccc gcgctccacc agcttcgagc gttccgaagc ctggcgcctg gccagcaccg
    11461 gcacgctgcg ctggcgccag cagtggcgcg actacgcgca gatcagcgat cacctcaagc
    11521 gcgccgtcat cgcgtccgag gacgacggct ttgccaacca cgagggggtg gactgggacg
    11581 ccatcgaaaa ggcctggcag cgcaacgccg ccgccgagga agccgcggcc gagcgcgcgg
    11641 cgcagaaccg gccggcgcgc ccgatccgca tccgtggcgg ctcgaccatc acccagcagc
    11701 tggccaagaa cctgctgctg tcgggcgagc gcagcctgtg gcgcaagggg caggagctgg
    11761 tgctcacctt cgcactggag cagctgctgg acaagcgccg catcctggag ctctacctca
    11821 accatgtgga gtggggcgag ggcgtgttcg gcgccgaagc ggcggcgcag cactacttcc
    11881 gcaagagcgc ggcgcaactg acggccgcgg agtcggcccg gctggccgtc atgctgccgc
    11941 gcccgcgcta tttcgagaag ctgccgcgtt cggcctacct ggccagccgc acccgcacca
    12001 tcttggcgcg catgcgcagc gccgaactgc cctgagcccg aaacactgca ccaggctgaa
    12061 ggtggcggcc tgcacccgcg cgcagaaagg aaaaagcccg ccaacggctg ggccgtggcg
    12121 ggccatggtg aaccgacccg gccgttcagg gccgggtgcg ccatcaccgg tcagggcttg
    12181 ctggccgtcg gaaacggcca cgctgcctgc gggctcagcg tggtttgcgc agccggcttc
    12241 ttggccgctg ctttcttggc tggcgcggct ttcttggcag ccggcttctt ggcgggcgcg
    12301 gccttcttgg ccggtgccgc tttcttggcg gctacctttt tggcgggcgc ggctttcttc
    12361 gccggtgcgg ccttcttggc cggtgccgct ttcttggcgg ctaccttttt ggcgggtgcg
    12421 gctttcttcg ccggtgcggc cttcttggcc ggcgcagctt tcttcgccgg tgccttcttg
    12481 gcgggcgcgg ccttcttggc cggtgccgct ttcttggccg gagcggcttt cttggccgga
    12541 gcggctttct tcgccggggc cttcttggcc ggagcggctt tcttcgccgg ggccttcttc
    12601 gcaggtgctt tcttcgcagt tgccattttt ttctcctcga tcaagttgaa cgttcaacag
    12661 caggaaacgc ttcacgcggg ttggcgcgtg cgaagcgatt catggtgctg gggcaggccc
    12721 caacaccatg aacaggggcg caccgaaccg acgacagcag gctgtcggcg gtcggggcga
    12781 atcgggtgca cattcggttc agcgggggtc agtcccacga cagcgcgccg ccggtctggt
    12841 actcgatgac gcgcgtttcg aagaagttgc gttccttttt cagatcgatc atctcgctca
    12901 tccaggggaa cgggttctcg gcgttggggt acagcgcgtc cagaccgatc tgggtggcgc
    12961 gccgattggc aatgtggcgc aggtagccct tgaacatgga ggcgttcatg cccagcacgc
    13021 cgcgcggcat ggtgtcttcg gcgtagcggt actccagctc gaccgcgtgc tcgaacaggg
    13081 cgcgcacctc ggccttgaag gccggcgtcc acaggtgggg gttttccagc ttgatctggt
    13141 tgatcaggtc gatgccgaag ttgcagtgca tcgactcgtc gcgcaggatg tactggtact
    13201 gctcggccgc gccgaccatc ttgttctgcc ggcccagcgc gaggatctgc gtgaagccga
    13261 cgtagaagaa caggccttcc atcaggcagg caaagacgat cagcgacttc agcaggcgct
    13321 ggtcggcctc gggcgtgccg gtgcggaaat cggggtccat gatcgcgtcg atgaacggga
    13381 tcaggaattc gtccttgtcg cggatcgacg ggatctcgtg gtaggcgttg aagatctcgc
    13441 cttcgtccag ccccagcgat tcgacgatgt actggtaggc gtgggtgtgg atggcttcct
    13501 cgaaggcctg gcgcagcagg aactgccggc actcgggcgc cgtgacgtgg cggtaggtgc
    13561 ccagcacgat gttgttggcg gccagcgagt cggcggtgac g
//
LOCUS       DAOQTZ010000077        13563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5127_length_13563_cov_13.514140, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000077 DAOQTZ010000000
VERSION     DAOQTZ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13563
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5127_length_13563_cov_13.514140"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKN51_09680"
     CDS             complement(<1..225)
                     /locus_tag="PKN51_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85409.1"
                     /translation="MGIPADLSLRWPSAAALEAPWWQPYRALLAAWPPPGLAPGAPVA
                     DGLNALAAAQGDVPVRFVGQQALPEGQAYEA"
     gene            445..1599
                     /locus_tag="PKN51_09685"
     CDS             445..1599
                     /locus_tag="PKN51_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT85410.1"
                     /translation="MPTDPRKRAFLRIAAASALGATALAGCGRKDEAGAPAAAPVAAP
                     PAAAGPLKAAWIYVGPVGNAGWSFAHDQGRLAVQAAFGDRVATSFVESVPEGADTERV
                     LRDLIGQGHTLIFGTSFGFMEAMVKLAQEHPQVRFEHATGYKTAPNLNVYDSRFYQDT
                     YLAGIIAGHMTQSGTLGYVGSVPIPEVLRNINAFTLGAQSVKPQIRTRVVWINSWYDP
                     PKETEAAQSLINAGADVLLQNTDSPAVIQAADKAGKLAFGWDSDMSAHGGAAHLASCI
                     ADWSHYYKKAVQAALDGSWKGGQVTRWGVPEGQNDLIKLSERIPAEVRAQVEAIKAKM
                     KDGSFDVFTGPVVDVAGKERLAAGQRADDAWMGAIDFFVKGVEGQIPASK"
     gene            1657..2658
                     /locus_tag="PKN51_09690"
     CDS             1657..2658
                     /locus_tag="PKN51_09690"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNT85411.1"
                     /translation="MNLERSLWHPVALGHVVGSQPRAVTLLGEDLVLWRDGNGHAHAW
                     MDQCPHRGARLSLGRVHGGQLECPYHGWRFDGGGRCTHVPSVPDFQPGPRHGARTFDT
                     REAYGMLWVRLAAPEGEPQPIHQLPVFAAEAEPRLRKTTSGPYLVAASAPRIIENFLD
                     MAHFGYVHEGWLGSRERPEIPPYEVQATPHGVRATGCLAWQPRSSIHAASGAQVEYTY
                     EVTAPYMAVLTKVPEAGSTAIEGMNESIAMFVCPVTEESSVAWTRMAMNDFDSPDARL
                     IDFQNTIFGQDQPVLESQRPKRLPLAPAAEAHCAADRMSAAYRRYLRESGITFGVIP"
     gene            2841..3896
                     /locus_tag="PKN51_09695"
     CDS             2841..3896
                     /locus_tag="PKN51_09695"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNT85412.1"
                     /translation="MSWAPPPIAAARLPALLAAAPKAELHIHIEGSLEPEMIFALAAR
                     HGIALPYADVQALRRAYAFGNLQQFLDLYYQGADVLRDEEDFYQLGRAYLARAATENI
                     VHAEIFFDPQTHTARGVPMGAVMRGLRRASDDGRRELGIGSALILCFLRHLSEREAFE
                     TLEAALPWRDDFIGVGLDSSELGHPPEKFARVFARCRELGLHVVAHAGEEGPPEYIWQ
                     ALDILKAERIDHGVQAVHDAALMRRLAQRRIPLTVCPLSNQKLQVFPDLAHHSLPQLL
                     AAGLCATVNSDDPAYFGGYVNANFQAVFAATGLDSAAAYQLAANSFEASFADAALKRR
                     WMDRLQAAFERAHDAGS"
     gene            3969..5042
                     /locus_tag="PKN51_09700"
     CDS             3969..5042
                     /locus_tag="PKN51_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT85413.1"
                     /translation="MYKNLVAALAAACCFFPLQPAQAQPPTRAAWVYVTPVLDTGWTR
                     QHELGRLAVQQALGDQVKTSYVENVAEGADAERVMRDLARQGNDIIFTTSFGYMEPAL
                     KLAQEFPRVKFEVITGVRRAPNVATANARYYEGRYLSGVAAGRMTKSNVVGYVAGFPI
                     PEILSSINAFTLGLRSVNPQATVKLVWLNEWFNPPREREAAMTLIDQGADVLAFNSAS
                     TAVMAAAEERGQLAIAYHSDMRAVAPTAQLAAVTHHWEAYDLARVRALRAGQWQSGDS
                     WGGVAAGMVRIGAFGPKVPAAVRQEVLARQKDIAAGRLQPFAGPVVDNTGRPRVPKGR
                     SASDAEILQMDYLVEGVQGSLPK"
     gene            5246..5812
                     /gene="dcd"
                     /locus_tag="PKN51_09705"
     CDS             5246..5812
                     /gene="dcd"
                     /locus_tag="PKN51_09705"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNT85414.1"
                     /translation="MSIKSDRWIRRMAEQHGMIEPYEPGQVREVDGRRIVSYGTSSYG
                     YDIRCAPEFKIFTNIHSTVVDPKNFDEKSFVDFHGDCCIIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVLFF
                     ESDEVCEVSYKDRGGKYQGQVGVTLPKT"
     gene            complement(5816..6721)
                     /locus_tag="PKN51_09710"
     CDS             complement(5816..6721)
                     /locus_tag="PKN51_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HNT85415.1"
                     /translation="MLLNPLDSAARLPWAALVDEIEALLGDPAVRVPERIILPTAQGA
                     VLFVMPACDAGIAMTKLISFTPANVGTARPTIQGDVTVFDVATGARRLILDGPTVTGR
                     RTAAVSALAARRLAPNRAGPMLVVGAGVQGQAHVEAFAAALGVREFRLASRRRTSAEA
                     LAVHARALGLRAEVVEDADAALADCPLVATCTPAREVVLRAMPRADAFIAAVGAFTPQ
                     MVELDAALCRAIAAQGRIVIDTPDAAHEAGDLIQAGVPVQGLPTLAQMVAADETPARG
                     PVLFKNCGWAGWDLAAARLALRSEA"
     gene            complement(6727..7413)
                     /gene="pcp"
                     /locus_tag="PKN51_09715"
     CDS             complement(6727..7413)
                     /gene="pcp"
                     /locus_tag="PKN51_09715"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HNT85416.1"
                     /translation="MVAPASTSRLTTASADRPVVLVTGFDPFGGDALNPSWLLAERLH
                     RRRVSGHVVQAAQLPTRFADSLPRLYALLAQWRPALVVCLGLAASRAALSIERVAINV
                     QDARIPDNAGAQPVDEPVVAGAPAAYFSTLPIKAMRRAMLAAGVPAEVSQTAGTFVCN
                     HVFYGLMHHLATRRGLQRTRGGFIHVPPLAEHGPQGLPLEQMVEGLRTGIRAALGTTG
                     DIRQGAGATD"
     gene            7454..8329
                     /locus_tag="PKN51_09720"
     CDS             7454..8329
                     /locus_tag="PKN51_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNT85417.1"
                     /translation="MRWEGERQSDNVEDRRSGGGGGGFGIGGRGIGIGTIAIALLAWG
                     VFGINPLTTIGVLSGGGGAPTQVQQGPAKAPPADDRNAAFVSTVLASTEDVWGQIFQQ
                     GGARYQQPRLVLFRGATPTACGTGQSAMGPFYCPGDQKVYLDMNFFDTMTRQLGAPGE
                     FARAYVVAHEVGHHVQNLLGVTGKVDSMRGRISERQQNQLSVRLELQADCYAGIWANR
                     SQQAKQWLDADDIESAVNAAQQIGDDTLQKRGQGYAVPDSFTHGSSAQRVRWFMQGYK
                     TGSVQACDTFNAQGL"
     gene            8559..9995
                     /locus_tag="PKN51_09725"
     CDS             8559..9995
                     /locus_tag="PKN51_09725"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT85418.1"
                     /translation="MSTLYSDLALADPLKRAVADMGYETMTPIQAQAIPVVLAGQDVM
                     GAAQTGTGKTAAFSLPLLQRLMKHETASVSPARHPVRALVLLPTRELAVQVADQVKQY
                     AKYTQLRSACVFGGMDMKPQIAELRQGVEVLVATPGRLLDHIEAKTAVLNQVEYVVLD
                     EADRMLDIGFLPDLQRILSHLPKNRTTLLFSATFSPEIKRLAESYLSNPVLIEVARPN
                     ATASTVEQHFYLLHEDDKRRAIRQVLKDKDLRQAFVFCNSKLGTARLARALERDGLKT
                     TALHGDKSQDERLKSLEAFKQGEVDLLVATDVAARGLDIKDVPAVFNYDVPFNPEDYV
                     HRIGRTGRAGATGVAVTLVTRHDQRLMGELEKLLGRKIEVEPIEFEEERARGRHNDGR
                     RRWQADETGDARDQARRERRPAERRPAPVSAPADPFFDRPYEAPSEPPEQPPAWEQKA
                     RPSRPGLSANIKPKRKLAALFKAPEPTH"
     gene            complement(10067..11326)
                     /locus_tag="PKN51_09730"
     CDS             complement(10067..11326)
                     /locus_tag="PKN51_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HNT85419.1"
                     /translation="MSAAPARPRDAAGMKRLALGLLLAAALLYALATALVPRHAGWGY
                     LAAFAEAAMVGAIADWFAVVALFRHPLGLPIPHTAIIAAGKDRIGENLASFIATHFLA
                     TPQVLDKLREFDAAGRLADWLAQPDHAERVARRLSAIGGWAVGALDDPRVRGFVADLA
                     RRGLQQIELTRLSGQLLQALTHQRRHQELLDGVLAQLARLLGQDRVQDAITETIAREI
                     RALKYVGLDQVAARLATRKVVVVVARTIIDMADDPAHQLRQRFDGMVDSCVSRLQDDP
                     VLQARGEQLKRDLLDHPALGTYVNRLWSELLAWLQADMGQEDSAIRHHIAQAARTLGE
                     RLAGDAAVRAWINEELQAQAPRLIERYREDIRRYIAARVRAWDAGEMTAELERHIGRD
                     LQFIRINGTLVGGLVGLAIHALTHLWR"
     gene            complement(11323..12165)
                     /locus_tag="PKN51_09735"
     CDS             complement(11323..12165)
                     /locus_tag="PKN51_09735"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNT85420.1"
                     /translation="MALYLIGDVQGCDAALGRLLDELAFSPSRDRLVLLGDLVNRGPR
                     SLAVLRRVQALGDAATCLLGNHDLHLLAVSLGVRPPHRSDTLADILAAPDRDALLDGL
                     RQRPLAWRERGVLMVHAGVLPQWTAAQTLALAGEVEAALRGPDWGQFMRQMYGNHPDA
                     WDDSLQGAARLRVIVNALTRLRFCTPDGRMEFATKEGGAAAAPPGHLPWFDVPGRRTA
                     GEVVAFGHWSTLGWLDRPDLISLDTGCVWGGCLSAVRLGTDGRQRELIQVRCPQAQQP
                     GGRA"
     gene            12201..13508
                     /locus_tag="PKN51_09740"
     CDS             12201..13508
                     /locus_tag="PKN51_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNT85421.1"
                     /translation="MDLLLIVFLTLLNGVFAMSELAVASSRKARLTAMEESGDAGASA
                     ALRLMEQPTQFLSTVQVGITSIGMLNGIVGEAAFSGPVADGLTRAGLSAGAASIAATA
                     LVVVAITFTTIVFGELVPKRIGQMFPETVARWIARPMGWLASAAKPFVALLSLCTQAT
                     LKLLRIDESHGRAVTEEEISASLEEGVDAGLIEEHEHQMVRNVFHLDDRSLISLMVPR
                     SEIDWLKAEASVAEALDFVAGQGERKAHSWYPVCDGGLDNVIGIISVARLLELGRHDQ
                     DLLRAHVIPPTFVPETLSALELLEQLRARSGRMMLVVDEYGVLQGLITQHDFLEAITG
                     ELNPGAHIEAWAIPLGSGGWQLDGMMPISEMKARLDIDGDLPHEDRGRYNTVAGLLMA
                     EAGRLPAVGDVIDCAGWSFEVTGLDGRRIDKVLARPLEISEST"
BASE COUNT         2222 a         4895 c         4597 g         1849 t
ORIGIN      
        1 ggcttcatac gcctggccct ccggcagcgc ctgctggccc acaaagcgca ccggcacgtc
       61 gccctgcgcc gccgccagcg cgttcaggcc gtcggctacc ggggcgcccg gcgccagccc
      121 cggcggcggc caggccgcca gcagcgcccg gtacggctgc caccacggcg cctccagggc
      181 cgcggcggac ggccagcgaa gcgacaggtc agcggggatg cccatggccc gattgtccgc
      241 ccctgcggcg ccggcgggcc ggcggccgtg ggctgcttct tgagtcaggt caattcaaca
      301 gaccgggcga cctacaatgg ccgtcgcgcc ccacgcccgg cttccatcgg cccgttcgac
      361 cccgcgtgca ggggcgcccg tcgacgcccg gcccgcgccg acggatccca cagatcgggc
      421 catgcccacc cctggagccg caccatgccc accgatccgc gcaaacgcgc cttcctccga
      481 atcgccgccg cctccgccct gggcgccacc gcactggccg gctgcggccg caaggacgag
      541 gccggcgcgc cggccgcggc cccggtcgcc gcaccaccgg ccgccgccgg gccgctgaag
      601 gccgcctgga tctacgtcgg cccggtcggc aatgccggct ggagctttgc ccacgaccag
      661 ggtcgcctgg cggtgcaggc cgcgttcggt gaccgggtcg ccaccagctt cgtcgagagc
      721 gtgcccgagg gcgccgacac cgagcgcgtg ctgcgcgacc tgatcggcca gggccacacc
      781 ctgatcttcg gcaccagctt cggcttcatg gaggccatgg tcaagctggc gcaggagcat
      841 ccgcaagtcc gcttcgagca cgccaccggc tacaagacgg caccgaacct gaacgtgtac
      901 gactcgcgct tctatcagga cacctacctg gccggcatca tcgccggtca catgacgcaa
      961 agcggcacgc tgggctacgt cggctcggtg ccgatccccg aggtgctgcg caacatcaac
     1021 gccttcaccc tgggcgcgca aagcgtcaag ccccagatca gaacgcgcgt ggtgtggatc
     1081 aacagctggt acgacccgcc caaggaaacc gaggcggcgc agtcgctgat caacgccggc
     1141 gccgacgtgc tgctgcagaa caccgattcg ccggccgtga tccaggccgc cgacaaggcc
     1201 ggcaagctgg ctttcggctg ggattcggac atgagcgcgc atggcggcgc ggcgcacctg
     1261 gcctcgtgca tcgccgactg gagccactac tacaaaaagg cggtgcaggc ggcgctggac
     1321 ggcagctgga agggcgggca ggtcacgcgc tggggcgtgc ccgaaggcca gaacgacctg
     1381 atcaagctgt cggaaagaat cccggccgag gtgcgcgccc aggtcgaggc catcaaggcc
     1441 aaaatgaagg acggcagctt cgacgtcttc accggcccgg tggtcgacgt ggcgggcaag
     1501 gaacgcctgg ccgccggcca gcgcgccgac gacgcctgga tgggcgccat cgacttcttt
     1561 gtcaaggggg tcgaaggcca gatccctgct tcaaaataaa tagctgctgg cgcaagctgg
     1621 atctgcgcca gacccctgtc ggatctggag gcgaagatga acctggaacg cagcctgtgg
     1681 caccccgtgg cgctcggcca cgtggtcggc agccagccgc gcgccgtcac cctgctgggc
     1741 gaggacctgg tgctgtggcg cgacggcaat ggccacgccc acgcctggat ggaccagtgc
     1801 ccacaccgcg gcgcgcgcct gtcgctcggc cgcgtgcatg gcggccagct ggaatgcccc
     1861 taccacggct ggcgtttcga cggcggcggc cggtgcaccc atgtgccgtc cgtgcccgac
     1921 ttccagcccg gcccgcgcca cggcgcgcgc accttcgaca cccgcgaggc ctacggcatg
     1981 ctgtgggtgc gtctggctgc gcccgagggc gagccgcagc cgatccatca gctgcccgtg
     2041 ttcgccgccg aagccgagcc gcgcctgcgc aagaccacca gcggccccta cctggtggcc
     2101 gccagcgcgc cgcgcatcat cgagaacttc ctcgacatgg cgcacttcgg ctacgtgcac
     2161 gaaggctggc tgggcagccg cgagcggccc gaaatcccac cctacgaggt gcaggccacg
     2221 ccgcacggcg tgcgcgccac cggctgcctg gcctggcagc cgcgctcgtc gatccacgcc
     2281 gcaagcggcg cccaggtcga atacacctat gaagtcaccg cgccctacat ggcggtgctg
     2341 accaaggtgc ccgaagccgg cagcaccgcc atcgaaggca tgaacgagtc gatcgccatg
     2401 ttcgtctgcc ccgtgaccga ggaaagcagc gtggcctgga cgcgcatggc gatgaacgac
     2461 ttcgactcgc ccgacgcgcg gctgatcgac ttccagaaca ccatcttcgg ccaggaccag
     2521 ccggtgctcg aatcgcagcg ccccaagcgc ctgccgctgg cgcccgccgc cgaagcgcat
     2581 tgcgccgccg accgcatgtc ggcggcctac cgccgctacc tgcgcgaaag cggcatcacc
     2641 ttcggcgtca tcccatgagc gcgccgggcc gccccaagcg cgaatcccgc agcgcgtcag
     2701 cgcggaggga taccgaatga gcgcgccggg ccgccccaag cgcgaatccc gcagcgcgtc
     2761 agcgcggagg gataccgaat gagcgcgccg ggccgcccca agcgcgaatc ccgcagcgcg
     2821 tcagcgcgga gggcgcacct gtgagctggg cgccgccacc catcgccgca gcgcgcctgc
     2881 cggcgctgct ggcggcagcc cccaaggccg agctgcacat ccacatcgaa ggctcgctgg
     2941 agccggaaat gatcttcgcg ctggcggcgc gccacggcat cgccctgccc tacgccgacg
     3001 tccaagcgct gcgccgcgcc tacgccttcg gcaacctgca gcagtttctg gacctgtact
     3061 accagggtgc cgacgtgctg cgcgatgagg aggattttta tcagttgggg cgcgcctacc
     3121 ttgcacgagc agctacagaa aacatagtac acgccgaaat cttcttcgac ccgcagacgc
     3181 acaccgcccg cggcgtgccg atgggcgccg tgatgcgcgg cctgcgccgc gccagcgacg
     3241 atggccggcg cgagctgggc atcggcagcg cgctgatcct gtgctttctg cgccacctgt
     3301 ccgagcgcga ggccttcgag acgctcgaag ccgccctgcc ctggcgcgac gacttcatcg
     3361 gcgtcgggct cgattccagc gagctgggcc acccgcccga aaaattcgcc cgcgtgtttg
     3421 cgcgctgccg cgagctgggc ctgcacgtgg tcgcccacgc cggcgaagaa ggcccgcccg
     3481 aatacatctg gcaggcgctg gacatcctga aggccgagcg catcgaccac ggcgtgcagg
     3541 cggtgcacga cgcggcgctg atgcgacggc tggcccagcg gcgcatcccg ctcaccgtct
     3601 gcccgctgtc caaccagaag ctgcaggtgt ttcccgacct ggcgcaccac agcctgccgc
     3661 agctgctggc cgctggcctg tgcgccaccg tcaacagcga cgacccggcc tacttcggcg
     3721 gttacgtcaa cgccaacttc caggcggtgt ttgccgccac cgggctggac agcgccgccg
     3781 cctaccagct ggcggccaac agcttcgaag ccagctttgc cgacgcggcg ctcaagcgac
     3841 ggtggatgga ccggctgcaa gccgccttcg agcgcgcgca cgacgcggga tcgtagaatc
     3901 gcaccccgca gcgctgccgc cccgacggcg ccgcgtcttc atccatcggg gccatgtaga
     3961 ggaacaccat gtacaaaaac ctcgtggccg cgctcgcggc cgcttgctgc ttcttccccc
     4021 tgcaacccgc acaagcccag ccgcccacgc gcgccgcctg ggtctacgtc acccccgtgc
     4081 tcgacaccgg ctggacgcgc cagcacgagc tggggcggct ggcggtgcag caggcgctgg
     4141 gcgatcaggt caagaccagc tatgtcgaga acgtggccga gggcgccgac gccgagcgcg
     4201 tgatgcgcga tctggcacgc cagggcaacg acatcatctt caccaccagc ttcggctaca
     4261 tggagccggc gctgaagctg gcgcaggagt tccccagggt gaagttcgag gtcatcaccg
     4321 gcgtcaggcg cgcgcccaac gtggccaccg ccaacgcgcg ctactacgaa gggcgctacc
     4381 tgtcgggcgt ggccgcgggg cgcatgacga agtcgaacgt ggtgggctac gtggcgggct
     4441 ttccgatccc ggagatcctg tccagcatca acgccttcac gctcggtctg cgcagcgtca
     4501 acccccaggc cacggtcaag ctggtttggc tgaacgagtg gttcaacccg ccgcgcgagc
     4561 gcgaggccgc gatgacgctg atcgaccagg gcgccgacgt gctggccttc aacagcgcct
     4621 ccaccgccgt tatggccgcc gccgaggagc gcggccagct ggccatcgcc taccactccg
     4681 acatgcgcgc cgtggcgccg acggcgcagc tggccgccgt gacccaccac tgggaggcct
     4741 acgacctggc ccgcgtgcgc gcgctgcgcg ccggccagtg gcagagcggc gacagctggg
     4801 gcggcgtggc cgccggcatg gtgcgcatcg gcgccttcgg ccccaaggtg cccgccgccg
     4861 tgcgccagga agtgctggcg cggcagaagg acatcgccgc cggacggctg cagccgtttg
     4921 ccggcccggt ggtcgacaac accggcaggc cgcgggtgcc caaggggcgc agcgccagcg
     4981 acgccgaaat cctgcagatg gactacctgg tcgaaggtgt gcagggcagc ttgccgaagt
     5041 agacatccga ccagcgaacg cagaggttgc ggaggtttcg caaagcgcac gaacagaaga
     5101 gcaacttcat ggacttgttc tgcgcatggc gcgcgatccc ggtgttcggc agaccgcgga
     5161 ttcagccgcc gacaaatcag gcaaaatacg gccccagcgc ttgtccattg tgcgcaagca
     5221 gctatcgatt taagagtgac gcagcatgag catcaagagc gacaggtgga tccgccgcat
     5281 ggccgagcag cacggcatga tcgagcccta cgagcccggc caggtgcgcg aggtggacgg
     5341 ccgcagaatc gtcagctacg gcaccagcag ttacggttac gacatccgct gcgcacccga
     5401 gttcaagatc ttcaccaaca tccacagcac cgtggtcgac ccgaagaact tcgacgaaaa
     5461 gagcttcgtc gattttcatg gcgactgctg catcatcccg cccaacagct tcgccctggc
     5521 gcgcacggtg gagtacttcc gcatcccgcg caacgtgctg accatctgcc tgggcaagag
     5581 cacctacgcg cgctgcggca tcatcgtcaa cgtcacgccc ttcgagcccg aatgggaggg
     5641 ctatgtgacg ctggaattca gcaacaccac gccgctgccg gccaagatct acgccggcga
     5701 gggctgcgcc caggtgctgt tcttcgaaag cgacgaggtg tgtgaagtca gctacaagga
     5761 ccgcggcggc aagtaccagg gccaggtcgg cgtgacgctg cccaagacct gaggctcaag
     5821 cctcagagcg cagcgccagc cgcgccgccg ccaggtccca gccggcccag ccgcagttct
     5881 tgaacagcac cggcccgcgc gccggtgttt cgtctgcggc caccatctgc gccagcgtgg
     5941 gcagaccctg caccggcacg ccggcctgaa tcaggtcgcc ggcctcgtgc gcggcgtcgg
     6001 gcgtgtcgat gacgatgcgg ccctgcgcgg cgatggcgcg gcacagggcg gcgtccagct
     6061 ccaccatctg cggcgtgaag gcgccgacgg cggcgatgaa ggcgtcggcg cgcggcatgg
     6121 cgcgcagcac cacctcgcgc gccggcgtgc aggtggccac cagcgggcag tcggccagcg
     6181 cggcgtcggc gtcttccacc acctcggcgc gcaggcccag cgcacgcgcg tggacggcca
     6241 gggcctcggc gctggtgcgg cggcgcgagg ccagtcggaa ctcgcgcacg cccagcgcgg
     6301 cggcaaaggc ctcgacatgc gcctgcccct gcacgccggc accgaccacc agcatcggcc
     6361 cggcccggtt cggtgccagg cggcgggccg ccagcgccga cacggcggcg gtgcgccggc
     6421 cggtcaccgt cggtccgtcc aggatcagcc gacgcgcgcc ggtggcgacg tcgaacaccg
     6481 tcacgtcgcc ctggatggtg gggcgcgccg tgcccacgtt ggcgggcgtg aagctgatca
     6541 gcttggtcat ggcgatgccg gcgtcgcagg ccggcatgac gaacagcacc gcgccctggg
     6601 cggtcggcag gatgatgcgc tcgggcaccc gcaccgccgg atcgcccagc agcgcctcga
     6661 tctcatcgac cagcgccgcc cagggcaggc gcgccgcgct gtcgagcggg ttcagcagca
     6721 tcggggtcag tccgtggcgc cggcaccctg acggatgtcg ccggtggtgc ccagcgccgc
     6781 ccggatgccg gtgcgcagcc cctccaccat ctgctccagc ggcaggccct gggggccgtg
     6841 ttctgccagc ggtggcacat gaatgaagcc gccgcgcgtg cgctgcaggc cgcgccgcgt
     6901 cgccaggtgg tgcatcaggc catagaacac atggttgcag acgaaggtgc ccgccgtctg
     6961 cgacacctcg gccggcacgc cggcggccag catggcccgg cgcatggcct tgatcggcaa
     7021 ggtgctgaaa taggccgccg gcgcgcccgc caccaccggc tcgtccaccg gctgcgcgcc
     7081 ggcgttgtca ggaatgcgcg catcctgcac gttgatggcc acgcgctcga tcgacagcgc
     7141 cgcgcggctg gccgccagcc ccaggcagac caccagcgcc gggcgccact gggccagcag
     7201 cgcgtacagg cgcggcagcg agtcggcaaa gcgcgtcggc aactgcgccg cctgcaccac
     7261 atggcccgag acgcggcgcc ggtgcaggcg ctcggccagc agccaactgg gattgagtgc
     7321 gtccccgcca aacggatcga agccggtgac gagcaccacc gggcggtccg ccgaggccgt
     7381 cgtgagccgg gatgtcgaag ctggcgctac catggcggca atttagcgca actcgcgcca
     7441 tggggagcgg cacatgagat gggaaggtga gcgtcagtcc gacaacgtgg aagatcgtcg
     7501 cagcggtggc ggcggaggtg gcttcggcat cggcggccgc ggcatcggca tcggcaccat
     7561 cgccattgcg ctgctggcct ggggtgtgtt cggcatcaac ccgctgacga cgatcggcgt
     7621 actgtccggc ggcggtggtg cgccgacgca ggtgcaacag gggccggcca aggcgccgcc
     7681 ggccgatgac cgcaacgccg ccttcgtgtc caccgtgctg gcctcgaccg aggatgtgtg
     7741 gggccagatc tttcagcagg gcggcgcgcg ctatcagcag ccgcgcctgg tgctgtttcg
     7801 cggcgccacg cccacggcct gtggcaccgg ccagtcggcc atggggccgt tctactgccc
     7861 gggcgaccag aaggtgtatc tggacatgaa cttcttcgac accatgacgc gccagctcgg
     7921 cgcgccgggc gagtttgcgc gcgcctacgt ggtggcgcac gaggtcggcc accatgtgca
     7981 gaacctgttg ggcgtgaccg gcaaggtcga ttcgatgcgc ggtcgcatca gcgaacgaca
     8041 gcagaaccag ctgtcggtgc ggctggagct gcaggccgac tgctacgccg gcatctgggc
     8101 caaccgctcg cagcaggcca agcagtggct ggacgcggac gacatcgaat cggccgtcaa
     8161 cgccgcccag cagatcggcg acgacacgct gcaaaagcgc ggccagggct acgccgtgcc
     8221 cgacagcttc acgcacggct ccagcgccca gcgcgtgcgc tggttcatgc agggctacaa
     8281 gaccggcagc gtgcaggcct gcgacacctt caacgcgcaa gggctttaag cgcaggggcg
     8341 tccgctcaaa caggcaggcg acggcagaag aactgaagga tttcgcagaa agcgcgcaag
     8401 aaaaggagga ttccacttct gcgtcttctg agcgaccttt gcgccctctg cattcggcgc
     8461 tcggtcttct cgcttcaccc ccgcggccaa gcttcggtac cccccacaag ccaccgttgc
     8521 acgcgcgcca cgcaccggtg cgacaatacg gggttcgcat gagcaccctc tattcagatc
     8581 tcgcgctggc cgatcccctc aagcgcgccg tggccgacat gggctacgag accatgaccc
     8641 ccatccaggc ccaggccatc cccgtggtgc tggcgggcca ggacgtgatg ggcgcggcgc
     8701 agaccggcac cggcaagacg gcggcgtttt cgctgccgct gctgcagcgc ctgatgaagc
     8761 acgagaccgc ctcggtctcg ccggcgcgcc acccggtgcg cgcgctggtg ctgctgccca
     8821 cgcgcgagct ggcggtgcag gtggccgacc aggtcaagca gtacgccaaa tacacccagc
     8881 tgcgcagcgc ctgcgtgttt ggcggcatgg acatgaagcc gcagatcgcc gaactgcgcc
     8941 agggcgtgga ggtgctggtg gccacgccgg ggcgcctgct ggatcacatc gaggccaaga
     9001 ccgccgtgct gaaccaggtc gagtacgtgg tgctggacga ggccgatcgc atgctggaca
     9061 tcggttttct gcccgacctg cagcgcatcc tgagccacct gccgaaaaac cgcaccaccc
     9121 tgctgttttc cgccaccttc tcgcccgaga tcaagcgcct ggccgaaagc tacctgagca
     9181 acccggtgct gatcgaggtg gcgcgcccca acgccaccgc gtccacggtg gagcagcatt
     9241 tctacctgct gcatgaagac gacaagcgcc gcgccatccg ccaggtgctc aaggacaagg
     9301 acctgcgcca ggccttcgtg ttctgcaaca gcaagctggg cacggcgcgg ctggcgcgcg
     9361 cgctggagcg cgacggcctg aaaaccaccg ccctgcacgg cgacaagagc caggacgaac
     9421 gcctcaaatc gctcgaagcc ttcaagcagg gcgaggtcga tctgctggtg gccaccgacg
     9481 tggccgcgcg cgggctggac atcaaggatg tgccggcggt cttcaactac gacgtgccct
     9541 tcaaccccga ggactacgtg caccgcatcg gccgcaccgg ccgcgccggc gccacgggcg
     9601 tggcggtgac cctggtcacc aggcacgacc agcgcctgat gggcgagctg gaaaagctgc
     9661 tgggccgcaa gatcgaggtc gagccgatcg agttcgagga agagcgcgcc cgcggccgcc
     9721 acaacgacgg ccgccgccgc tggcaggccg acgagaccgg cgacgcgcgc gaccaggccc
     9781 ggcgcgagcg ccgtccggcc gagcgtcgcc cggcccccgt cagcgccccg gccgacccgt
     9841 tcttcgaccg cccctacgaa gccccgagcg agccgcccga gcagccgccg gcctgggagc
     9901 agaaggcgcg ccccagccgc cccggcctgt cggccaacat caagcccaag cgcaagctgg
     9961 cggcgctgtt caaggcgccg gagccgaccc actgagccgc tttgttgcca aataggcctc
    10021 tggcgcgcac ccagcaagcg ccaacagcta tctatttcat agcacttcag cgccacaaat
    10081 gggtcagggc gtggatcgcc agtcccacca gaccgcccac cagggtgccg ttgatgcgga
    10141 tgaactgcag gtcgcgcccg atgtggcgtt ccagctcggc ggtcatctcg cccgcgtccc
    10201 aggcccgcac gcgggcggcg atgtagcggc ggatgtcctc gcggtagcgc tcgatcaggc
    10261 gtggcgcctg cgcctgcagc tcctcgttga tccaggcccg cacggcggcg tcgcccgcca
    10321 ggcgctcgcc cagcgtgcgc gcagcctggg cgatgtggtg acggatggct gaatcttcct
    10381 gccccatgtc ggcctgcagc caggccagca gctcgctcca caagcggttg acgtaggtgc
    10441 ccagggccgg gtggtcgagc aggtcgcgct tgagctgctc gccacgcgcc tgcagcaccg
    10501 ggtcgtcctg cagccgcgac acgcagctgt ccaccatgcc gtcaaagcgc tggcgcaact
    10561 ggtgcgccgg gtcgtcggcc atgtcgatga tggtgcgcgc caccaccacc accaccttgc
    10621 gcgtggccag gcgcgcggcc acctgatcca gcccgacgta cttgagcgcc cggatctcgc
    10681 gtgcgatggt ttcggtgatg gcgtcctgca cccgatcctg ccccagcagc cgcgccagct
    10741 gcgccagcac gccgtcgagc agctcctggt ggcggcgctg gtgggtcagg gcctgcagca
    10801 gctgcccgga caggcgcgtc agctcgatct gctgcaggcc gcgccgcgcc aggtcggcca
    10861 cgaaaccgcg cacgcgcgga tcgtccagcg cgcccacggc ccagccgccg atggcgctca
    10921 accgccgtgc cacgcgctcg gcatggtcgg gctgggccag ccagtcggcc agccggccgg
    10981 cggcgtcgaa ctcgcgcagc ttgtccagca cctgcggcgt ggccagaaaa tgcgtggcaa
    11041 tgaagctggc caggttctcg ccgatgcggt ccttgccggc cgcaatgatg gcggtgtgcg
    11101 ggatcggcag gccaagcggg tggcgaaaca gcgccaccac ggcaaaccag tcggcaatcg
    11161 cgcccaccat ggcggcttcg gcgaacgccg ccaggtagcc ccagccggca tggcgcggca
    11221 ccagcgccgt ggccagggcg tacagcaggg ccgcggccag cagcaggccg agcgccagcc
    11281 gcttcatgcc ggcggcgtcg cgcgggcgag ccggtgcggc gctcacgccc ggccacccgg
    11341 ctgctgcgcc tgcgggcagc gcacctgtat cagctcgcgc tgacgtccat ccgtccccag
    11401 gcgcacggca ctcaggcagc caccccagac gcaaccggtg tcgagcgaga tcagatccgg
    11461 ccggtccagc cagcccaggg tcgaccagtg accgaaggcc accacctcgc cggccgtgcg
    11521 gcgcccgggc acatcgaacc agggcaggtg gccgggaggt gcggcggcgg cgccgccctc
    11581 cttggtggcg aactccatgc gaccgtcggg cgtgcaaaag cgcagccgcg tgagggcgtt
    11641 gacgatgacc cgcagccgcg cggcgccctg taggctgtcg tcccaggcat ccgggtgatt
    11701 gccgtacatc tggcgcatga actggcccca gtcggggccg cgcagcgccg cctcgacctc
    11761 gccggccagc gccagcgtct gcgccgccgt ccactgcggc agcacgccgg catgcaccat
    11821 cagcacgccc cgctcgcgcc aggccagcgg ccgctggcgc aacccgtcca gcagcgcatc
    11881 gcggtcgggc gcggccagga tgtcggccag ggtgtcgctg cggtgcggcg gacgcacgcc
    11941 cagcgacacc gccagcaggt gcaggtcgtg gttgcccagc aggcaggtgg cggcgtcgcc
    12001 cagtgcctgc acgcgccgca gcacggccag cgaccgcggg ccgcggttga ccaggtcgcc
    12061 cagcagcacc aggcggtcgc gactgggaga aaacgccagt tcgtcgagca ggcggcccag
    12121 ggcggcgtcg cagccctgca cgtcgccgat caggtagagt gccatctccc gattttctcc
    12181 ccggcacacc gccggccggc atggacctgc tgctgatcgt cttcctcacc ctgctcaatg
    12241 gcgtgttcgc catgtcggaa ctggccgtgg cctccagccg caaggcgcgg ctgacggcca
    12301 tggaagaaag cggcgacgcc ggcgccagcg cagccctcag gctgatggaa cagccaacgc
    12361 agtttctgtc caccgtgcag gtgggcatca cctcgatcgg tatgctcaac ggcatcgtcg
    12421 gcgaagccgc cttcagcggc ccggtggccg acgggctgac ccgcgccggc ctgagcgcgg
    12481 gtgccgcctc catcgccgcc acggcgctgg tggtggtggc catcaccttc accaccatcg
    12541 tgttcggcga actggtgccc aagcgcatcg ggcagatgtt tccggaaacc gtggcgcgct
    12601 ggatcgcgcg ccccatgggc tggctggcaa gcgccgccaa gcccttcgtg gcgctgctgt
    12661 cgctgtgcac gcaggcgacg ctgaaattgc tgcgcatcga cgagtcgcac gggcgtgccg
    12721 tcaccgagga agagatttcg gccagcctgg aagagggcgt ggacgccggc ctgatcgaag
    12781 aacacgagca ccagatggtg cgcaacgtgt ttcacctgga tgaccgttcg ctgatctcgc
    12841 tgatggtgcc gcgcagcgaa atcgactggc tcaaggccga ggccagcgtg gccgaggcgc
    12901 tcgattttgt cgccggccag ggcgaacgca aggcccactc ctggtatccg gtctgcgatg
    12961 gcggtctgga caacgtgatc ggcattatct cggtggcccg cctgctggaa ttggggcgcc
    13021 atgaccagga tttgctgcgc gcccatgtga tacccccaac tttcgtgccg gaaaccctca
    13081 gcgcgctcga attgctggaa cagctgcgtg cccgctccgg ccgcatgatg ctggtggtgg
    13141 acgaatatgg cgtgctgcag ggcctgatca cccagcatga tttcctggaa gccatcaccg
    13201 gcgaactcaa cccgggcgcc catatcgagg cctgggccat ccccctgggc agcggcggct
    13261 ggcaactgga cggcatgatg ccgatttcgg aaatgaaggc ccggctcgac atcgacggcg
    13321 acctgccgca cgaagaccgc ggccgctaca acaccgtggc cggcctgctg atggccgaag
    13381 ccggccggtt gccggccgta ggcgacgtca tcgactgcgc cggctggtct ttcgaggtga
    13441 ccgggctgga tgggcgtcgc atcgacaaag tgctggcccg cccgctggaa atatccgaat
    13501 ccacctgagc cattcgatta ttgattcggg gttgccgatc ggatatattc cgaccgccca
    13561 tat
//
LOCUS       DAOQTZ010000078        13320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5237_length_13320_cov_12.975876, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000078 DAOQTZ010000000
VERSION     DAOQTZ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5237_length_13320_cov_12.975876"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..153)
                     /gene="ccoS"
                     /locus_tag="PKN51_09745"
     CDS             complement(16..153)
                     /gene="ccoS"
                     /locus_tag="PKN51_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNT85422.1"
                     /translation="MDILYLLIPLSVVLVFFILAALWWAIYRGQFDDVEREGERIFEH
                     D"
     gene            complement(153..2486)
                     /locus_tag="PKN51_09750"
     CDS             complement(153..2486)
                     /locus_tag="PKN51_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNT85423.1"
                     /translation="MQGASGTFPGSVAGDGPGQRPSGPPQGDAAWQLLDERDEWLEFS
                     RPAGAQGGLWESSVVFEGMHCAACAVTIEQALRATPGVREADISAASHRGRVVWEEGA
                     TRPSAWMQAVLRSGYRPLPAHDAFASERRQAETRRMLWRLGVAGVCMMQVMMYATPTY
                     LASPGEIEPNFIHLLRWAQWVLSIPVVLFSCQPFFRHALLDLKLRRVSMDLPVALGML
                     ITFVVSSLGTFEPEGLFGREVYFDSLTMFVFFLLTGRWLELRLRDRTAGALEALMHRL
                     PDSVLRQAADGAWERVSVRRVRVDDVLRVLPGEVFPADGVLLRGQTAVDEALLTGEST
                     PLSRGEGAPVIAGSHNLSAAVEMRVEQVGADTRYAQIVALMAQASTSKPQIAQLADRL
                     AKPFLIFVLLAAGLACAWWWPGDPAHALMIAVAILVVTCPCALSLATPAAMLASAGAL
                     ARRGVLVRRLSALEALSKVDTVVFDKTGTLTRDAFVLEQVRLRDGVARAQALALAAAM
                     AQGSLHPLSRALVQAAQDEGVEPLAVADLQEMVGQGLTARGPATGPLRGQVRLGAAAW
                     CGVQGSDAGGPVSHLSDEQGWVASFEMREHLRPDARAAVEALRAAGVRVRLLSGDRPD
                     AVRRVGQLAGIDDVQGSCLPDDKLAQMRQAQGAGAVVAMVGDGLNDGPVLAGADVSFA
                     FGHAVPLARAQADFVVLGERLGLISGSFAVARRTMRIVRQNLGWAAAYNLVGVPLAMA
                     GWMSPWAAGLGMAASSLLVVVNALRLTKAPPMPHPEH"
     gene            2637..3080
                     /locus_tag="PKN51_09755"
     CDS             2637..3080
                     /locus_tag="PKN51_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT85424.1"
                     /translation="MYDRILVATDGSKLSKKAVDSAIKLAALCGAELIALRVVPRYPQ
                     SYFEGSIPLAPDDVARVEKQWADQGQESVDAVKKAGEAKGVKVKAILAKSDIVSDALI
                     AAARKHKADIIVMASHGRKGVKRLLLGSETQQVLTHSEIPVLVLR"
     gene            complement(3172..3783)
                     /locus_tag="PKN51_09760"
     CDS             complement(3172..3783)
                     /locus_tag="PKN51_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNT85425.1"
                     /translation="MSLRALLLLLVGTAIVAFIGINWSAMTAPTDLNLVFMEIRAPLG
                     LVLLGLMALLSAVFVGLIAYTQGTVLMETRRHAKELSVQRELADKAEASRFTELRAHL
                     DREVSRLSEVIANQTRETLSRVDRAETGLRDRPVDAEIGRLVQTVEGHNRELHARIDR
                     LEIGLRERLAGQLPAPTPRPTALPNAAPLQAGGGESAGAASAS"
     gene            complement(3894..5468)
                     /locus_tag="PKN51_09765"
     CDS             complement(3894..5468)
                     /locus_tag="PKN51_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HNT85426.1"
                     /translation="MSQQNLFVALRDAFPADLDAVAVETDNGLCYSWRDLDRASAMVA
                     NLLASLSIPPDASGAPPRVAVQVEKSVEAMILYLATLRAGFVFLPLNTAYQRAEIEYF
                     IGNARPAVVVCTRGNFPWVSQIAFQAGTRHVFTLDDDRSGSLLERAAHCSDRQQPVVR
                     QADDLAAILYTSGTTGRSKGAMLSHGNLLSNAAMLKEYWGWRTPEQGGDVLIHALPIF
                     HVHGLFVAIHGALLNGSRMLWHGKFDPRKVIADLPRATVFMGVPTLYVRMLAEPALTR
                     AQAAHMRLFISGSAPLLIETFNDWRARTGHTILERYGMSETIMLTSNPWQADARYGGQ
                     DERRGGTVGFPLPGVRLRVQGEDGRDLPAGEIGGIQVKGPNVFAGYWQMPEKTAEEFT
                     SDGFFKTGDVGQQDARGYVTIVGRSKDLIISGGYNVYPAEIEGFLNELPGVAESAVVG
                     VPHPDFGEVGVAVVIARPGARPDADDLLAELKARLANFKIPKRCFVETELPRNTMGKV
                     QKNLLRQRYQHLFVTA"
     gene            complement(5493..6290)
                     /locus_tag="PKN51_09770"
     CDS             complement(5493..6290)
                     /locus_tag="PKN51_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT85427.1"
                     /translation="MACGSIELTVDGAIATVVLSHPGKFNAMSRAMWRRLRELFLELP
                     ARADLRCVVVRGEGAQFCAGGDIAEYPAFRFQPDRLRAFHEDEVWGALGALLDCDLPL
                     VAAIAGHCMGAGVEIASCCDIRVAAADARFGAPIARLGFPMAPREAQLVARALGEATA
                     RELLLEAAVLDAAEMQARGFLQRVLPDAAAMHAHALERARRIAALAPQAARLNKQTLR
                     ALNGPSALAGQAPAAIENIVHWAYDYADNAEHREGIDAFLAKRPARF"
     gene            complement(6271..7257)
                     /locus_tag="PKN51_09775"
     CDS             complement(6271..7257)
                     /locus_tag="PKN51_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HNT85428.1"
                     /translation="MITSFTRRQLLLATAAVTALPGGARAQSAWPARPVNLVVPFPAG
                     GGTDAFARPLGAQFTRLTGQNLVIDNKGGGGGTVGAAVAAKAPADGYNFFMGGVHHAI
                     APSVYPKLQYDLLQDFVPLILVARVPQVVVVNPKRVQARTVKDLIELARKNPGKLNYA
                     SAGSGTSHHLAGELFKQQTGTFITHIPYRGAGPALQALIAGEVDLMFDGLGSSAAHIK
                     GGRIQPLMMAGRSRSPAFPDVPSARESGLSDYDVSTWYGIWAPKGTPTEAQAAMIEAV
                     RKSAQTPENKQVWAGQGAEFANLDAAAFGGFVQLEMAKWAKVVKATGGVRID"
     gene            complement(7282..8682)
                     /locus_tag="PKN51_09780"
     CDS             complement(7282..8682)
                     /locus_tag="PKN51_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HNT85429.1"
                     /translation="MEADRDPAAATGDRPGLSMRERLSAMLERGVDAFGGWNARRFLR
                     ELQQVLDPAVSEVEGGRRAAAVAEEYARATPQQRRACWLLMSEHFSPDVSAIQAAHAA
                     YQAARGSEDESRAEVRLRRAFTAPRTRLLQRFSAYPGGVRFLVDLRAELLPHLARDKR
                     LIALDDELQELFSTWFDVGFLELRRISWDSPASLVEKLIRYEAVHDIRSWADAKNRLD
                     EDRRCYGFFHPRLPDEPLIFVEVALLHDMARSITPLLDEQAPAADLTRAHTAIFYSIS
                     NTQAGLRGVSFGDSLIKRVVETLRTEFAQLKVFATLSPIPGLRRWVDQQAPALLRATP
                     ARLRQALERELGAPLAPDAAALLKALDGVATLDERSAARRWLLAVAARYLGRELADDG
                     RPLDGVARFHLGNGARIEQLNWLADPSPKGLRQSWGLMVNYLYDLRRLDRHRAQLAQG
                     KPPMSHAVQALQTDLL"
     gene            complement(8749..9588)
                     /gene="lgt"
                     /locus_tag="PKN51_09785"
     CDS             complement(8749..9588)
                     /gene="lgt"
                     /locus_tag="PKN51_09785"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT85430.1"
                     /translation="MLVHPQIDPVALQLGPLAIHWYGLTYLVAFGLFMLLALRRLHHP
                     PYAAITQPAPWTRRDIEDMLFWGVLGVILGGRLGYCLFYKPAYYAANPLEILAVWKGG
                     MSFHGGMLGVIAAMALWARTRGRPWLQVTDLVAPCVPLGLASGRVGNFINGELWGRVA
                     DPSLPWAMVFPGAVLPDGSNPPRHPSQIYQFLLEGVLLFALLWLYARRPRQTGQVSGA
                     FLLGYGVLRFVAEYFRQPDAHLGLLALGMSMGQWLCLPMMAAGLALWVWAGRRPAPLA
                     GTP"
     gene            9746..10219
                     /gene="bfr"
                     /locus_tag="PKN51_09790"
     CDS             9746..10219
                     /gene="bfr"
                     /locus_tag="PKN51_09790"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNT85431.1"
                     /translation="MQGHPEVIDCLKALLRGELTARDQYFLHSRIYEDQGLVRLYERM
                     DHEMQEETQHADAILRRILLLGGQPDMRPRELNPGVDVPDMLRKDLAAEYQVRDNLRQ
                     AIALCDRLHDYVSRDLLLAQLRDTEQDHAYWLEKQIGLIEQIGLQNYLQSQARVD"
     gene            complement(10311..12170)
                     /gene="ilvD"
                     /locus_tag="PKN51_09795"
     CDS             complement(10311..12170)
                     /gene="ilvD"
                     /locus_tag="PKN51_09795"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNT85432.1"
                     /translation="MPAYRSKTSTHGRNMAGARALWRATGMKDADFKKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDVIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVIFVSGGPMEAGKVRLSVPGQ
                     ATVTITKKLDLIDAMVMAADDKASDADVAEVERSACPTCGSCSGMFTANSMNCLTEAL
                     GLALPGNGTVVATHADREQLFRRAGRRIVELARQYYEGGDDRVLPRSVGFKAFENAMT
                     LDIAMGGSTNTILHLLAIAQEAEIAFTMQDIDRLSRVVPQLCKVAPNTDKYHVEDVHR
                     AGGIFAILGELERAGKLHTDVPTVHAPTLGQALAQWDVLRTQDAAVHHFFKAGPGGVP
                     TQVAFSQDARWPSLDLDRAGGCIRSCDHAFSQEGGLAVLRGNIALDGCVVKTAGVDES
                     ILVFEGPAHVVESQDEAVEHILGDQVKAGDVVIVRYEGPKGGPGMQEMLYPTSYIKSK
                     GLGKACALLTDGRFSGGTSGLSIGHCSPEAAAGGAIGLVRNGDRIRIDIPNRSINVLV
                     SDDVLARRRAEQDAVGWKPAQSRPRKVSAALKAYAKLVTSADKGAVRDLSLLD"
     gene            12415..>13320
                     /locus_tag="PKN51_09800"
     CDS             12415..>13320
                     /locus_tag="PKN51_09800"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNT85433.1"
                     /translation="MTDSAPLNRRSAHITQGKARAPNRSMYYGMGYTEADFDKPMVGV
                     ANGHSTITPCNSGLQKLADAAVEGIREAGGNAQTFGTPTISDGMAMGTEGMKYSLVSR
                     EVIADCVETCVQGQWMDGCVVIGGCDKNMPGGMMGMLRANVPSIYVYGGTIEPGCLHG
                     CDLNIVSVFEAVGENAAGKISDKELKDIEQHAIPGTGSCGGMYTANTMSSAFEALGMS
                     LPYSSTMANPHQEKVDSARESARVLIEAIRRDLKPRDIVTRQSIENAVAVIMAVGGST
                     NAVLHFLAIAHAAGVEWSIDDFERMR"
BASE COUNT         2182 a         4714 c         4504 g         1920 t
ORIGIN      
        1 tttgattgac gtgcgtcaat catgctcgaa aatccgctcg ccctcgcgct cgacgtcgtc
       61 gaactggcca cggtagatgg cccaccacaa cgcggcgagg atgaaaaaca ccagcaccac
      121 cgacagcgga atcagcagat aaagaatgtc catcagtgct ccggatgcgg catcggcggg
      181 gccttggtca gcctcagggc attgaccacc accagcaacg aactggccgc catgcccaag
      241 cctgccgccc agggcgacat ccaccctgcc atggcgagcg gcacgcccac caggttgtag
      301 gccgcggccc agcccaaatt ctgcctcacg atgcgcatgg tgcggcgcgc cacggcaaaa
      361 gaccccgaaa tcagccccag gcgctcgccc agcaccacga agtcggcctg cgcccgcgcc
      421 agcggcacgg cgtgcccgaa cgcgaacgac acgtcggcgc ccgccagcac cggcccgtcg
      481 ttcaagccgt cgccgaccat ggccacgacc gccccggcgc cctgggcctg acgcatctgc
      541 gccagcttgt cgtcgggcag acagctgccc tgcacatcgt cgatgccggc cagctggccg
      601 acgcgccgca ccgcgtccgg ccggtcgccc gacagcagcc gcaccctgac gccggccgcg
      661 cgcaacgcct cgaccgcggc gcgcgcgtcc gggcgcagat gctcgcgcat ctcaaagctg
      721 gccacccagc cctgctcgtc gctcagatga ctgaccggac cgcccgcgtc gctgccctgc
      781 acgccgcacc atgccgccgc ccccaggcga acctgaccgc gcagcggccc ggttgcaggg
      841 ccacgggccg tcaggccctg ccccaccatt tcctgcaaat cggccaccgc caggggctcg
      901 accccctcgt cctgcgccgc ctgcaccagc gcgcgcgaca gcggatgcag gctgccctgc
      961 gccatggccg ccgccaacgc cagcgcctgc gcgcgcgcca cgccgtcgcg caggcgcacc
     1021 tgctccagca cgaaggcatc gcgcgtcagc gtgccggtct tgtcgaacac caccgtgtcc
     1081 accttcgaca gcgcttccag tgccgacagg cgacgcacca gcacgccacg gcgcgccagc
     1141 gcgccggcgc tggccagcat ggcggcgggc gtcgccagcg acagcgcgca cgggcaggtc
     1201 accaccagga tcgccaccgc gatcatcagc gcatgcgccg gatcgccggg ccaccaccag
     1261 gcgcaggcca ggccggccgc cagcagcacg aagatgagaa atggcttggc caggcggtcg
     1321 gccagttggg caatttgcgg cttgctggtc gacgcctggg ccatcagggc cacgatctgc
     1381 gcgtagcggg tgtcggcgcc gacctgctcg acccgcatct ccaccgctgc cgacaggttg
     1441 tggctgccgg caatgacggg cgcaccctcg ccccgcgaca gcggggtcga ttcgccggtc
     1501 agcaaggctt cgtcaaccgc ggtctggccc cgcagcagca ccccatcggc cggaaacacc
     1561 tcgcccggca gcacccgcag cacgtcgtcg acgcgcacgc gccgcaccga cacgcgctcc
     1621 catgcgccgt ccgccgcctg tcgcagcacg ctgtcgggca ggcggtgcat cagggcttcc
     1681 agcgcgcccg ccgtgcgatc gcgcagacgc agctccagcc agcggccggt gagcaggaag
     1741 aacacgaaca tggtcagcga gtcgaaatag acctcgcgcc cgaacagccc ctcgggctcg
     1801 aacgtgccca gcgagctgac cacgaaggtg atcagcatgc ccagcgccac cggcaggtcc
     1861 atgctgacgc ggcgcagctt caggtcgagc agcgcatgtc gaaagaaggg ctgacacgag
     1921 aacagcacca ccggaatcga cagcacccac tgcgcccagc gcagcaggtg gatgaagttc
     1981 ggttcgatct cgccgggcga cgccaggtag gtcggcgtgg catacatcat cacctgcatc
     2041 atgcagacac cggccacccc caggcgccac agcatgcggc gggtttcggc ctgacggcgc
     2101 tcgctggcga aggcatcgtg ggccggcagc ggccggtagc cactgcgcag caccgcctgc
     2161 atccaggcgg aaggacgggt ggcgccctcc tcccacacca cccgaccgcg atggctggcg
     2221 gcgctgatgt cggcttcgcg cacgccgggc gtggcgcgca gggcctgttc gatggtcacc
     2281 gcgcaggccg cgcaatgcat gccttcgaac accacactgg attcccacag tcccccctgc
     2341 gcgcctgccg ggcggctgaa ctccagccac tcgtcgcgtt cgtcgagcaa ctgccacgcg
     2401 gcatcgccct gcggcggccc cgatggccgc tgcccgggcc catcgcccgc caccgagccc
     2461 gggaaagtcc cacttgcgcc ttgcatcacg gcacgaactg tacgcgtttt ggcgcctgcg
     2521 ccggccaggg cgcgccaatg accgcccacc gcgatcggag tgcgctaagc tatccgctga
     2581 tccgcgcaag ctccccggcg ccgcccgccg atacctcaac actggagatc cccaccatgt
     2641 acgaccgcat ccttgtcgcc accgacggct ccaaactgtc caagaaagcg gtggacagcg
     2701 ccatcaagct ggccgccctg tgtggcgccg aactcattgc gctgcgcgtc gtgccgcgct
     2761 acccgcagag ctacttcgag ggctccatcc cgctggcgcc ggacgacgtg gcgcgcgtcg
     2821 aaaagcaatg ggccgaccag ggccaggagt ccgtggacgc ggtcaagaag gccggcgagg
     2881 ccaagggcgt caaggtcaag gcgattctgg ccaagtccga catcgtctcc gatgccctga
     2941 tcgctgcagc caggaagcac aaggccgaca tcatcgtcat ggcctcgcac ggccgcaaag
     3001 gcgtcaagcg cctgctgctg ggcagcgaga cgcagcaggt gttgacgcac tcggaaatcc
     3061 cagtgctggt gctgcgctga ccgtgcgccg gccgccgcgg ccgcgcaccc ccccgaacga
     3121 cggctccggg cgccacccgg ggccgtcgtc acatgcggcc agcccgctgc gtcatgacgc
     3181 gctcgccgcg cccgccgatt cgccaccacc ggcttgcagc ggcgccgcat tgggcagtgc
     3241 ggtgggcctt ggggtcgggg cgggcagctg cccggccagg cgctcgcgca gcccgatctc
     3301 gagccggtcg atgcgggcgt gcagctcgcg gttgtggcct tccacggtct gcaccaggcg
     3361 gccgatctcg gcgtccaccg gacgatcgcg cagccccgtt tcggcccggt cgacgcgcga
     3421 cagcgtctcg cgtgtctggt tggcgatgac ctcggacagc cggctcacct cgcggtccag
     3481 gtgggcgcgc agttcggtga agcgcgaggc ctcggccttg tcggccagtt cacgctgcac
     3541 cgacagctcc ttggcgtgac ggcgcgtttc catcagcacc gtgccctggg tgtaggcgat
     3601 caggccgacg aacacggccg acagcagcgc catcagcccc agcagcacca gaccgagcgg
     3661 ggcgcggatt tccatgaaca ccaggttcag gtcggtgggc gccgtcatgg cgctccagtt
     3721 gatgccgatg aaggccacga tggcggtgcc gaccagcagc agaagcaggg ctcgcaggga
     3781 catggcggtg gggctccttc ctgggaatct gcacgcggct cgcgtgacca tcagcgggca
     3841 agtttacccg tgcccctgcc acgcctgaca gccgcgcctg acggtcgcaa gcttcacgcc
     3901 gtgacgaaca gatgctggta gcgctggcgc agcaggttct tctgcacctt gcccatggtg
     3961 ttgcgcggca gttcggtttc gacgaagcag cgcttgggga tcttgaagtt ggccagccgc
     4021 gccttcagct cggccagcag gtcatcggcg tcgggccggg cgccgggccg ggcgatcacc
     4081 acggccacgc cgacttcgcc gaagtccggg tgcggcacgc cgaccacggc gctttcggcc
     4141 acgccgggca gctcgttcag aaagccttcg atctcggccg ggtagacgtt gtagccaccg
     4201 ctgatgatca ggtccttgct gcgcccgacg atggtgacgt agccgcgcgc gtcctgctgg
     4261 ccgacgtcgc cggtcttgaa gaagccatcg gacgtgaact cctcagcggt cttttcgggc
     4321 atctgccagt agccggcgaa cacgttcggg cccttgacct ggatgccgcc gatttcgccc
     4381 gccggcagat cgcgaccgtc ttcgccctgc acacgcaggc gcacgcccgg cagcggaaag
     4441 ccgaccgtgc cgccgcgccg ctcgtcctgg ccgccgtagc gggcgtcggc ctgccagggg
     4501 ttgctggtca gcatgatggt ttcgctcatg ccgtagcgct ccaggatggt gtggccggtg
     4561 cgcgcgcgcc agtcgttgaa ggtttcgatc agcagcggcg ccgaaccgct gatgaacagc
     4621 cgcatgtggg ccgcctgcgc ccgggtcagc gcgggttcgg ccagcatgcg cacgtacagc
     4681 gtgggcacac ccatgaacac ggtggcgcgc ggcaggtcgg caatcacctt cctgggatcg
     4741 aacttgccgt gccacagcat gcggctgccg ttgagcaggg cgccgtgaat ggccacgaac
     4801 aggccatgca cgtgaaagat cggcagcgca tggatcagca cgtcgccgcc ctgctccggc
     4861 gttctccagc cccagtactc cttcagcatc gcggcgttgg acagcaggtt gccgtgcgac
     4921 agcatggcgc ccttgctgcg cccggtggtg ccgctggtgt agaggatggc cgccagatcg
     4981 tcggcctggc gcaccaccgg ctgctggcgg tcgctgcagt gcgcggcacg ctccagcagg
     5041 ctgccgctgc ggtcgtcgtc cagcgtgaac acgtggcgcg tgccggcctg aaaggcgatc
     5101 tggctgaccc aggggaagtt gccgcgggtg cacaccacca cggctggcct ggcgttgccg
     5161 atgaagtatt cgatctcggc gcgctggtag gcggtgttga gcggcaggaa cacgaagccg
     5221 gcacgcagcg tggccaggta caggatcatg gcctcgaccg acttttccac ctgcaccgcc
     5281 acgcgcggcg gcgcgccgct ggcatcgggc gggatggaca gcgaggccag caggttggcg
     5341 accatggcgc tggcgcgatc gaggtcgcgc cacgaatagc acaggccgtt gtcggtttcg
     5401 acggccacgg cgtccagatc ggcgggaaag gcgtcgcgca gggcaacgaa caggttttgc
     5461 tgggacatgg ggttcagggg taggagggaa gctcagaaac gcgccgggcg ctttgccaga
     5521 aaggcgtcga tgccctcgcg gtgctcggcg ttgtcggcgt agtcgtaggc ccaatgaact
     5581 atgttttcga tagctgccgg cgcttgccca gcaagcgccg aaggcccatt cagtgcacga
     5641 agggtctgct tgttcaggcg cgccgcctgc ggcgccaggg cggcgatgcg gcgggcacgc
     5701 tcgagcgcat gggcgtgcat ggccgcggcg tcgggcagca cgcgctgcag aaagccgcgc
     5761 gcctgcattt ctgcggcatc gagcaccgcc gcctccagca gcagctcgcg cgccgtggcc
     5821 tcgcccagcg cccgcgccac cagctgcgct tcgcgcggtg ccatcggaaa gcccaggcgg
     5881 gcgatcgggg cgccaaagcg cgcatcggcg gcggcgacgc gaatgtcgca gcagctggcg
     5941 atttccaccc ccgcgcccat gcagtggccg gcaatggcgg ccaccagcgg caggtcgcag
     6001 tccagcagcg cgcccagcgc gccccacacc tcgtcctcgt ggaaggcgcg cagacggtcg
     6061 ggctgaaagc ggaacgccgg gtattcggca atgtcgccgc cggcgcagaa ctgcgcaccc
     6121 tcaccacgca ccaccacgca gcgcaggtcg gcccgcgcgg gcagctccag gaacagctcg
     6181 cgcagccggc gccacatggc gcgcgacatg gcgttgaact tgccgggatg cgacagcacg
     6241 acggtcgcga tggcgccgtc gacggtcaac tcaatcgatc cgcacgccac cggtcgcctt
     6301 caccaccttg gcccacttgg ccatttcgag ctgcacgaaa ccgccgaagg cggcggcgtc
     6361 gaggttggcg aattccgccc cctgaccggc ccagacctgc ttgttttcgg gggtctgggc
     6421 ggacttgcgc acggcctcga tcatggccgc ctgggcctcg gtgggcgtgc ccttgggggc
     6481 ccagatgccg taccaggtgc tgacgtcgta gtcggacagc cccgactcac gcgccgaggg
     6541 cacgtccgga aaggcggggc ttcggctgcg cccggccatc atcagcggct ggatgcggcc
     6601 acccttgatg tgcgccgccg acgagcccag cccgtcgaac atcagatcca cctcgcctgc
     6661 gatcagggcc tgcagcgccg ggccggcgcc gcggtaggga atgtgggtga tgaaggtgcc
     6721 ggtctgctgt ttgaacagct cgccggccag atggtgcgag gtgccgctgc cggccgacgc
     6781 gtagttgagc ttgcccgggt tcttcctggc cagctcgatc aggtccttga cggtgcgcgc
     6841 ctgcacccgc ttggggttga ccaccaccac ctgcggcacg cgcgccacca gaatcagagg
     6901 cacgaagtcc tgcagcaggt cgtactggag cttggggtac acgctgggcg caatggcgtg
     6961 gtgcacgccg cccatgaaga agttgtagcc gtcggccggc gccttggccg ccaccgcggc
     7021 gcccaccgtg ccgccgccac cgcccttgtt gtcgatcacc aggttctgcc cggtcaggcg
     7081 cgtgaactgg gcgcccagcg ggcgcgcaaa ggcgtcggtg ccgccaccgg ccggaaaggg
     7141 caccaccagg ttgacgggcc gcgccggcca ggccgattgc gcgcgggcgc cgcccggcag
     7201 ggcggtgacg gcggcggtgg ccagcagcag ttgccgacgg gtgaacgacg tgatcatgcg
     7261 catctcctct ttcgtttgaa gtcaaagcag atcagtttgc aatgcctgca cggcgtgcga
     7321 catcgggggt tttccctgtg ccagctgggc ccgatgccga tccagacgcc gcaggtcgta
     7381 caggtagttg accatcaggc cccaggactg gcgcaggccc ttgggcgaag ggtcggccag
     7441 ccagttgagc tgctcgatgc gcgcgccgtt gcccaggtga aagcgcgcca cgccgtccag
     7501 cgggcggccg tcgtcggcca gctcgcgccc caggtagcgc gccgccaccg ccagcagcca
     7561 gcggcgcgcg gccgagcgct cgtccagcgt cgccacaccg tccagcgcct tcagcagggc
     7621 cgcggcgtcg ggcgccagcg gcgcgcccag ctcgcgctcc agcgcctgcc gcaggcgcgc
     7681 cggcgtggcg cgcagcagcg ccggcgcctg ttggtcgacc cagcggcgca ggccggggat
     7741 gggcgacagg gtggcaaaca ctttcagctg cgcaaactcg gtgcgcaggg tttccaccac
     7801 gcgcttgatc agcgagtcgc caaaactcac cccgcgcagg ccagcctggg tgttgctgat
     7861 ggagtagaag atggccgtgt gggcacgcgt cagatcggcg gccggcgcct gctcgtccag
     7921 cagcggggtg atgctgcggg ccatgtcgtg cagcagcgcc acctcgacga agatcagcgg
     7981 ctcgtcaggc aggcgcgggt gaaagaagcc gtagcagcgc cggtcttcgt cgagccggtt
     8041 cttggcgtcg gcccagctgc ggatgtcgtg caccgcctcg tagcggatca gcttttcgac
     8101 cagcgaggcg ggcgaatccc agctgatgcg ccgcagctcc agaaagccga cgtcgaacca
     8161 ggtggaaaac agctcctgca gttcgtcgtc gagcgcgatc aggcgcttgt cccgggccag
     8221 atggggcaac aactcggcgc gcaggtcgac cagaaagcgc acaccgcccg gataggcgga
     8281 aaagcgctgc agcaggcgcg tgcgcggcgc ggtgaaggcg cggcgcagcc gcacctcggc
     8341 gcgcgactcg tcttcgctgc cgcgcgcggc ctggtaggcg gcgtgtgcgg cctggatcgc
     8401 cgacacgtcg gggctgaagt gctcgctcat cagcagccag catgcgcgcc gctgctgcgg
     8461 cgtggcgcgg gcgtattcct cggccaccgc ggcggcgcgg cggccgccct cgacctcgct
     8521 gaccgccggg tcgagcacct gctgcagctc gcgcagaaag cggcgcgcgt tccagccgcc
     8581 gaaggcatcg acgccacgtt ccagcatggc gctcaggcgc tcgcgcatgc tgagccccgg
     8641 acggtctccg gtggccgctg cagggtcgcg atctgcttcc atattcatag ctgttggcgc
     8701 ttgatggata agcgccgaag ccgaaaatcg ctcatggtcc gcaagccgtc aaggcgtgcc
     8761 cgccagcggc gccggtcgcc gcccggccca gacccacagg gccaggccgg cggccatcat
     8821 gggcaggcac agccactggc ccatgctcat gcccagggcc agcagcccca ggtgggcgtc
     8881 gggctgtcgg aagtactcgg caacgaagcg cagcacccca tagcccagca gaaaggcgcc
     8941 gctgacctgg ccggtctggc gcggccggcg cgcgtacagc cacagcagcg cgaacagcag
     9001 cacaccctcc agcaggaact ggtagatctg cgacgggtgg cgcggcggat tgctgccgtc
     9061 gggcagcacc gcgccgggaa acaccatggc ccagggcagc gaggggtcgg ccacccgccc
     9121 ccacagctca ccgttgatga agttgccgac gcgtcccgaa gccaggccca gcggcacgca
     9181 gggcgccacc agatcggtca cctgcagcca gggccgcccg cgcgtgcgcg cccacagcgc
     9241 catggcggcg atcacgccca gcatgccgcc gtgaaaactc atgccgccct tccacaccgc
     9301 caggatctcc agcgggttgg ccgcgtagta cgctggcttg tagaacaggc agtaacccag
     9361 ccgaccgccc aggatcacgc ccagcacgcc ccagaacagc atgtcctcga tgtcgcgccg
     9421 cgtccacggc gccggttgcg tgatggctgc gtagggcggg tggtgcaggc gccgcagcgc
     9481 cagcagcatg aacaggccga acgccaccag ataggtcagc ccataccagt gaatggccag
     9541 cgggccgagc tgcaacgcga cgggatcgat ctgggggtgc accagcatgg cggcgattgt
     9601 gccgcaggcc agccggcatc ctccccggtg gcgccaccgc tgcgcaccga cgcgtggtgc
     9661 cgtgaacacg gtttgcgcca gcacagggtc tgaccacgcc gatgacgcga tactgacggc
     9721 cctgaccttc ccgaagagaa cccccatgca aggccatccc gaagtcatcg attgcctcaa
     9781 ggcgctgctg cgcggcgaac tgaccgcgcg cgaccagtac tttctgcatt cccgcatcta
     9841 tgaagaccag ggcctggtgc gcctgtacga gcgcatggac cacgaaatgc aggaggaaac
     9901 ccagcacgcc gacgccatcc tgcgccgcat cctgctgctg ggcgggcagc ccgacatgcg
     9961 gcccagggag ctcaaccccg gcgtcgacgt gcccgacatg ctgcgcaagg acctggcggc
    10021 cgagtaccag gtgcgcgaca acctgcgcca ggccatcgcc ctgtgcgacc gcctgcacga
    10081 ttacgtcagc cgcgacctgc tgctggcgca gctgcgcgac accgagcaag accacgccta
    10141 ctggctggaa aagcagatcg gcctgatcga gcagatcggg ttgcagaact acctgcagag
    10201 ccaggcccga gtcgactgag agcgccccac cgacgcctcg ccccacgatc cgaatcaaaa
    10261 cagcttccag cgcttgatgg gcaagcgcca gaagctatat ttttgatagt tcaatcgagc
    10321 agcgacaggt cgcgcaccgc ccctttgtcg gcgctggtca ccagtttggc gtaggccttg
    10381 agcgcggccg acaccttgcg cgggcgcgac tgggcgggct tccagccgac ggcgtcctgc
    10441 tcggcgcggc ggcgggccag cacgtcgtcg ctgaccagca cgttgatgct gcggttggga
    10501 atatcgatgc ggatgcggtc gccgtttcgc accaggccga tggcgccgcc ggccgctgcc
    10561 tcgggcgagc aatggcctat cgacaggccc gaggtgccgc ccgagaagcg cccgtcggtc
    10621 aacagcgcgc aggccttgcc caggcccttg ctcttgatgt agctcgtcgg gtagagcatt
    10681 tcctgcatgc cggggccgcc cttggggcct tcgtagcgca cgatgaccac gtcgcccgcc
    10741 ttgacctggt cgcccaggat gtgctcgacg gcctcgtcct ggctctcgac cacatgggcc
    10801 ggaccctcga acaccagaat ggattcgtcg acgcccgcgg tcttgaccac gcagccgtcg
    10861 agggcgatgt tgccgcgcag caccgccagg ccgccttcct ggctgaaggc gtggtcgcag
    10921 gagcggatgc agccgcccgc gcggtccagg tccaggctgg gccagcgcgc gtcctggctg
    10981 aaggccacct gcgtgggcac gccgcccggg ccggccttga agaagtggtg caccgccgca
    11041 tcctgggtgc gcagcacgtc ccactgcgcc agcgcctgcc ccagggtggg ggcatgcacc
    11101 gtcggcacgt cggtgtgcag cttgccggcg cgctccagct cacccaggat ggcaaagatg
    11161 ccaccggcac ggtgcacgtc ctcgacgtgg tatttgtcgg tgttgggcgc caccttgcac
    11221 agctgtggca cgacgcgcga gaggcggtcg atgtcctgca tggtgaaggc gatctcggcc
    11281 tcctgggcga tcgccagcag gtgcaggatg gtgttggtcg agccgcccat ggcgatatcg
    11341 agcgtcatgg cgttctcgaa ggccttgaag cccaccgaac gcggcagcac gcggtcgtcg
    11401 ccgccctcgt agtactggcg cgccagctcc acgatgcgcc ggccggcgcg cctgaacagc
    11461 tgttcgcggt cggcgtgcgt ggccaccacc gtgccgttgc ccggcagcgc caggcccagc
    11521 gcctcggtca aacagttcat ggagttggcg gtgaacatgc ccgagcacga gccgcaggtc
    11581 gggcaggcgc tgcgctcgac ctcggccacg tcggcgtcgc tggccttgtc gtcggcggcc
    11641 atgaccatgg cgtcgatcag gtcgagcttc ttggtgatgg tcaccgtcgc ctggccgggc
    11701 acgctcagcc tgaccttgcc ggcctccatc gggccgccgg agacgaagat cacggggata
    11761 ttgagccgca tcgcggccat gagcatgccg ggggtgatct tgtcgcagtt gctgatgcag
    11821 accatggcgt cggcgcaatg cgcgttgacc atgtattcca cgctgtcggc aatcacgtcg
    11881 cggctgggca gcgagtacag catgccgtca tggcccatgg cgatgccgtc gtccacggcg
    11941 atggtgttga actccttggc cacgccgccg gcggcctcga tctcgcgcgc gaccagctgg
    12001 cccaggtcct tcaggtgcac atggccgggc acgaactggg taaaggagtt gaccaccgcg
    12061 atgatgggct ttttaaaatc ggcgtccttc atgccggtgg cgcgccacag ggcgcgggca
    12121 ccggccatgt tgcggccatg ggtggatgtc ttggaacggt atgcgggcat cgtgggtagg
    12181 tgaaaaatgg gcaagaggaa ttctgtggcc cgtgcgcttg ggggccggtc cgcaccgcag
    12241 cacagggcct cttgggcttc ttgggcctgt tgggccggtt tgcgggcaaa ccaattgatt
    12301 atgtcgcagc gctgcaatcc ttacccgcgc cgggtgcgcc ggcgcgcgca ctggacaggc
    12361 ggggcgcagg cgcccacact tggccccatc accgcattgc gcgaggactg cagcatgacc
    12421 gattccgcgc ccctgaaccg ccgctcggcc cacatcaccc agggcaaggc gcgcgcgccc
    12481 aaccgctcca tgtattacgg catgggctac accgaggccg acttcgacaa gcccatggtc
    12541 ggcgtggcca acggccacag caccatcacg ccctgcaaca gcggcctgca gaagctggcc
    12601 gatgcggccg tcgagggcat acgcgaggcg ggcggcaacg cccagacctt tggcacgccc
    12661 acgatcagcg acggcatggc catgggcacc gagggcatga agtacagcct ggtaagccgc
    12721 gaggtcatcg ccgactgtgt cgagacctgc gtgcagggcc agtggatgga cggctgcgtg
    12781 gtcataggcg gctgcgacaa gaacatgccc ggcggcatga tgggcatgct gcgcgccaac
    12841 gtgcccagca tctatgtgta cggaggcacc atagagcccg gctgcctgca cggctgcgac
    12901 ctgaacatcg tgagcgtgtt cgaggccgtg ggcgagaacg ccgcgggcaa gatcagcgac
    12961 aaggaattga aggacatcga gcagcacgcc atccccggca cgggttcgtg cggcggcatg
    13021 tacacggcca acaccatgtc ctcggccttc gaggccctgg gcatgagcct gccctactcc
    13081 tccaccatgg ccaatccgca ccaggaaaag gtcgattccg cacgcgagtc ggccagggtg
    13141 ctcatagagg ccatacgccg cgacctcaaa ccccgcgaca tcgtcacgcg ccagtccatc
    13201 gagaacgccg tggccgtgat catggccgtg ggcggctcca cgaacgccgt gctgcacttt
    13261 ctggccatcg cccacgcggc gggcgtcgaa tggtccatcg acgacttcga gcgcatgcgc
//
LOCUS       DAOQTZ010000079        13220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5291_length_13220_cov_12.574630, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000079 DAOQTZ010000000
VERSION     DAOQTZ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5291_length_13220_cov_12.574630"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..>1330
                     /locus_tag="PKN51_09805"
     CDS             360..>1330
                     /locus_tag="PKN51_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNT85434.1"
                     /translation="MHTITLQIAAELKVRQAQVEAAITLLDGGATVPFIARYRKEATG
                     GLDDVQMRELEARLAYLRELHERRAAILRSIEEQGKLTAQLRAALEAAPTKQELEDLY
                     LPYKPKRRTKGQIAREFGLEPLADRLWGDPTLDPAREAQGFTRAEKGEGGEDFGTVQA
                     VLDGVRDILSERWAEDAALVQSLREWLWADGLLQSKLAAGRNEADAEVAKFRDYFDYD
                     EPIARVPSHRALAVFRGRQLEILDVKLVIPDAEPAPAATKTVASIGLAEGRIALHLGW
                     SHQGRAADDLLRRCVVWTWRVKLQLSTERELLARLREDAEQVAIKVFG"
     assembly_gap    1331..1430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1431..2550
                     /locus_tag="PKN51_09810"
     CDS             <1431..2550
                     /locus_tag="PKN51_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNT85435.1"
                     /translation="AGQAPAAIEKIVISEAGASVYSASEYASQELPDVDVSLRGAASI
                     ARRLQDPLAELVKIDPKAIGVGQYQHDVNQSALARQLDAVVEDCVNGVGVDLNTASAP
                     LLARVSGLSPAVARAVVRWREQHGAFRSRQQLLEVSGLGLKTFEQSAGFLRIRGGDNP
                     LDVTGVHPETYPLVEQIILKAGRPIEQLMGRAELLRSLRPEQFVTEAFGAVTVRDILA
                     ELEKPGRDPRPDFKVARFNEGVEDIADLKPGMVLEGTVSNVAAFGAFVDLGVHQDGLV
                     HVSQLAHKFVQDAREVVKTGQIVKVKVLEVDAARQRISLTMKLDAAAPRRDAPRDNRF
                     EPAGRPARPGHAAGRERGSAAATAMASAFEKPRGHQAK"
     gene            complement(2655..4925)
                     /locus_tag="PKN51_09815"
     CDS             complement(2655..4925)
                     /locus_tag="PKN51_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNT85436.1"
                     /translation="MSSSTAHPHVHPHRHAHDHAHEHGHDHPHADHDCGGAACEVAAR
                     PIDFSALAAGGALLLRIPDMDCASEEAQIRRALEGQAAVRGLRFDLGARTLAVDAPDS
                     AWSAVEQAIAAAGLKTERLSQPTSAADTRRRQRRQATQLAAALLLALGAELLHFVAPD
                     TRPWQFASMALAALAIALSGFGVYRKGLAALARGQLGISALMTVAVTGAFLIGQWTEA
                     AMVMALYSIAELIEARAVDRARHAIGGLLALTPPLAEVRQPDGGWLTQRADSVASGAL
                     VRVRPGERLALDGRVTAGHSAIDQAPITGESVPVEKAPGDEVFAGTINQHGALEYEVT
                     APASDTVLARIIHAVEQAQGTRAPTQRLVDRFAAVYTPTVFAIALAVALLGPWLAGWP
                     WLTALYKALVLLVIACPCALVIATPVTVVSGLAAAARRGILIKGGAYLEEARRLRHIA
                     LDKTGTLTEGRPRLVARELVDDRQEEARVWQLAASLAARSDHPVSRAIAQGLGNEPLA
                     VHDFGAAAGRGSNGRIHDQALVLGNHRWIEERGQCSPALEARMQAHEALGRSVSLLAD
                     DRGVLALFAVADALKPDTPAAVQQLRALGVEPIMLTGDNPTTAQAIAAQAGIGDVRAG
                     LLPQHKLEAITALNAHSPGTVGMVGDGINDAPALAAAALGFAMGQGGTHVASEAADVV
                     IMNDDLRRVPETVRLSRRTHAILWQNIALALGIKAVFLLLAVLGSATMWMAVFADMGA
                     SLLVVFNGLRMLRWRG"
     gene            5033..5497
                     /gene="cadR"
                     /locus_tag="PKN51_09820"
     CDS             5033..5497
                     /gene="cadR"
                     /locus_tag="PKN51_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HNT85437.1"
                     /translation="MKIGDLAQASATGVETIRYYERQGLLPAPARTAGNFRVYDEAHL
                     ERLRFIRQCRGLDMSLDEVRELLAVRDAPDADCGAANRVLDEHIGHVSRRIRELRALE
                     RQLKALRASCAAVQPSGDCGILVGLSSALERPVDAPLRSARHLGAVHGQGGG"
     gene            complement(5591..6727)
                     /locus_tag="PKN51_09825"
     CDS             complement(5591..6727)
                     /locus_tag="PKN51_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNT85438.1"
                     /translation="MALIQYLTQIEFDFGALARIGEHCQRVGITRALVVTDAGVRAAG
                     VLQPVLDALGPRAVAVFDDVPSNPTEAAVRGAVAQYQQHGCDGLIAVGGGSPIDCAKG
                     AAIAATHPGPLTHYATIEGGSERISAAAAPLIAVPTTAGTGSEVARGAIVIVDDGRKL
                     GFHSWHLMPRAAICDPALTLGLPPKLTAATGMDAIAHCMEVFMAPAFNSPADGIALEG
                     LRRGWAHIERATHDGSDREARLNMMAASIHGAMGFQKGLGCVHSLSHSLGGADPRLHH
                     GTLNAVFLPAVIGFNAEAESMRREQRLARMAQAMGLPPGADVADAVRAMTARLGLPSG
                     LAAMGVSQNQFDGVIAHALLDHCHKTNPRIASADDYRRMLHASM"
     gene            6800..7705
                     /locus_tag="PKN51_09830"
     CDS             6800..7705
                     /locus_tag="PKN51_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HNT85439.1"
                     /translation="MKSRTLHSLALAAALALLAGCASTSAPVVADTPGCLSNVAVDRL
                     LADYNARRPAANPPETLTMADAHCSRSKLQQRLAGQAGRLVGYKAGLTNPAVQKRFNT
                     DQPVWGALYSGMILNSDVTLDASFGSRPLYEADLLVRVKDRAINDASTPAQVLAHVDQ
                     VIPFIELPDLMVEAPPKINGAVLSALNVGTRLGVRGAPLLVPADAAGQARLLDQLRDM
                     NVRLVDGQGAALGGGKGSDVLGHPLNAVVWLAKALKAQGLSLQPGQVVSLGSFSALLP
                     PKPGLKVTVHYDGVPGLQPATVNFR"
     gene            7782..8765
                     /locus_tag="PKN51_09835"
     CDS             7782..8765
                     /locus_tag="PKN51_09835"
                     /inference="COORDINATES: protein motif:HMM:NF022664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HNT85440.1"
                     /translation="MFRCFKGIALAWAALALTPAAQAQWQPVGPALPPGGGQIVQCES
                     LDGDFRECRTPFGGPPMLVNQLSASNCVEGQTWGSRAPGTVWVARGCRGQFAAAYVGQ
                     PPGSIVVPGGGGSYDERSAVRCESDDGRYRECRAPVGGRLSLLRQESQTPCIEGQTWG
                     NRGSGTVWVNAGCRGVFGVAGWGGGGGSSETYRCESRDDRYNECRGPTRGRQVLVRQL
                     SESACIEGRSWGSRNGGVWVQYGCRGEFAAAGGWGGGGNWGQGGGRYEVECTSEDRRY
                     TQCNWDSRQGRPYVVEQYSSDACIEGSSWGYDRRRGNLWVDRGCRARFGAR"
     gene            8880..9797
                     /locus_tag="PKN51_09840"
     CDS             8880..9797
                     /locus_tag="PKN51_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT85441.1"
                     /translation="MRLLVERMARAPYPPMHTLTAAEAKAAHAASIGVLDVPKPRLPR
                     VQQLQIPARDGAALPARLVAPSLAAGLPVLLYLRGGGFTIGSVESHDTVCRVLAEQSG
                     AAVLSLDYRLAPEHRFPTAVHDAWDALRWLAGEGAAALALDGARIAVGGDSAGGTLAA
                     VSAIHARDLGLPLALQLLIYPGTTDHQDTDSHALYAQGPVLDKALIDWFFHHYIDSVD
                     RSDWRFAPLKSAEVDGVAPAWVGLAECDPLVDEGLLYADKLRAAGVPVELEIYRGVVH
                     GFMTMGRSIVEARQLHADAAAALRRAFER"
     gene            9883..10746
                     /locus_tag="PKN51_09845"
     CDS             9883..10746
                     /locus_tag="PKN51_09845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HNT85442.1"
                     /translation="MKPADFRFHHPLRVRWAEVDLQQIVFNPHYLTYYDCAMADYWRA
                     LALPYEATLRRLQGDIFLRKTAVEFNASAQLDDRLDVALRCERVGHSSITFTGAIFRD
                     QRLLNTAELVYVFADPASQRSRAVPAPLRALFAAYEAGERLTDVQLGPWSQLAEPARA
                     VRSAVFIEEQGIAHDEEWDALDADAVHAVVFNRLGVPVGTGRLLGEPQAGTARIGRMA
                     VDRALRGGGVGRLLIEALQQAAAGRGDRRVVLSAQRSAEGFYRRLGYAPVGQPYDEVG
                     IPHIGMQRDLV"
     gene            complement(10769..11533)
                     /locus_tag="PKN51_09850"
     CDS             complement(10769..11533)
                     /locus_tag="PKN51_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT85443.1"
                     /translation="MKLHILSDLHLSAGGLAHPHTDADVVVLAGDIARPAAAAAWARG
                     FGKPVLYVIGNHEFYGSSLPEVRAELRRLCAGTPVHLLDNDALVLGGVRFLGSTLWTD
                     FRAHGEGERQAEAMARSAAFMYDFRRVRVAAAADAPMLTPQDTVRLFEHNTLWLAAQL
                     AQPFAGPTVVITHHAPSTGSIHPRFAGSPFNAAFVSAAEHLLDGQRAALWVHGHTHDS
                     HDYRVNGTRVLCNPRGYVKDGVTENARFDPRLVVEV"
     gene            11601..12635
                     /locus_tag="PKN51_09855"
     CDS             11601..12635
                     /locus_tag="PKN51_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT85444.1"
                     /translation="MLAYILKRLLLMLPTLLGVLLLTFVVIQFVPGGPVEQYLAEAKA
                     GVGGAAGAEGGGLAYRGAQGVDPKRLEQIKALYGFDQPAHQRFVQMLGRFARFDLGTS
                     FFQNKAVWALIREKLPVSISLGLWTFFISYGISVPLGVAKAVRAGSRFDLATTLLVLV
                     GYAIPGFVLGVALLVIFGGQLQWFPLRGLTSPNFDELSLGGKIVDYLWHITLPVTSMV
                     LGSFAVTAMLTKNSFLEEIRKQYVLTARAKGLTERQVLWKHVFRNALIPIITGFPAAF
                     IGAFFTGSLLIETLFSLDGLGLLSYESVIRRDYPVVLGTLYLFTLIGLVTKLISDLTY
                     VWVDPRVKFD"
     gene            12640..>13220
                     /locus_tag="PKN51_09860"
     CDS             12640..>13220
                     /locus_tag="PKN51_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT85445.1"
                     /translation="MVTASVSPARRAWRRFRRNRLGLWSLVLFGVLVGFSLLAELVSN
                     DKPLLVRYQGQWYVPLVKTYPEKVFGGDFETETDYLDPFIRQQFSQPGNWAIYPPNPY
                     GARTINYFAKAPNPSAPSRDNWLGTDDRGRDLLAQLIYGFRVSVLFALALTAVGVLLG
                     VAAGAVQGFFGGRIDLAFQRFIEIWGSMPELYLL"
BASE COUNT         1907 a         4503 c         4738 g         1972 t
ORIGIN      
        1 cagcacctgc gtggccgcct cgacatcgag agcgttgcgg gcgaaggcac gcgggtaacc
       61 ctgcaggtgc ccatggagtc actgacgttc ccggagacgg tctgatgtcg tgtgaacgga
      121 tcccgcatac cggcattcat cgcctcaacc cacccgtccg gtagtgaccg caatcgccgc
      181 actctgagaa cctgtccgca ggccatgggg tgctgcgccg gggcgccccg ttcgggtcgt
      241 cggcaggtct tcacattccc tgggtcctgc gccgtcgcag gcgccgatgc ctgtggcaaa
      301 ctcgccagct ttttttggcg ccacccaccc cgtgcgcggg gcagcggcgc gaggcaggca
      361 tgcacaccat cacgttacag atcgccgcag agctgaaagt ccgccaggcc caggtcgagg
      421 ccgccatcac gctgctcgat ggcggcgcca cggtgccctt catcgcccgc taccgcaagg
      481 aagccacggg cggcctggac gacgtgcaga tgcgcgagct ggaggcgcgc ctggcctatc
      541 tgcgcgagct gcacgaacgg cgcgccgcca tcctgcgaag catcgaagag cagggcaagc
      601 tgacagcgca attgcgcgcc gccctggaag ccgcgccgac caagcaggag ctggaagacc
      661 tgtacctgcc ctacaaaccc aagcgccgca ccaagggcca gatcgcgcgc gaattcggcc
      721 tcgaaccgct ggccgaccgc ctgtggggcg acccgacgct ggaccccgcc cgtgaggcgc
      781 agggcttcac ccgggcggaa aaaggcgagg gcggagagga cttcggcacc gtgcaggcgg
      841 tgctggacgg cgtgcgcgac atcctgagcg agcgctgggc cgaagacgcc gccctggtgc
      901 agtcgctgcg cgaatggctg tgggccgacg gcctgctgca gagcaagctg gccgccggca
      961 ggaacgaggc cgatgccgag gtggccaagt ttcgcgacta cttcgattac gacgagccga
     1021 tcgcccgcgt gcccagtcac cgggcgctgg cggtgtttcg cggccgccag ctcgaaatac
     1081 tggatgtgaa actggtgatt cccgacgcgg agcctgcgcc agcagctacc aaaaccgtag
     1141 catcgatcgg gctggccgaa gggcggatcg ccctgcatct gggctggtcg caccagggcc
     1201 gcgccgccga cgacctgctg cgtcggtgcg tggtctggac ctggcgcgtc aagctgcaac
     1261 tgtccaccga acgcgagctg ctggcccgcc tgcgcgaaga cgcggagcag gtggcgatca
     1321 aggtgttcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgctgggcaa
     1441 gcgccagcag ctatcgaaaa gatagttatc agcgaagccg gtgcctccgt ctacagcgcc
     1501 agcgaatacg ccagccagga gctgccggac gtcgacgtga gcctgcgcgg cgccgccagc
     1561 attgcgcgtc gcctgcagga cccgctggcc gagctggtga agatcgaccc caaggcgatc
     1621 ggcgtcggcc agtaccagca cgacgtcaac cagagcgcgc tggcgcgcca gctcgacgcg
     1681 gtggtggaag actgcgtcaa cggcgtcggc gtggacctga acaccgccag cgcgccgctg
     1741 ctggcgcggg tgtcgggcct gagcccggcg gtggcgcgcg ccgtggtgcg ctggcgcgag
     1801 cagcacgggg ccttcaggag ccgccagcag ttgctggaag tcagcggcct ggggctcaag
     1861 accttcgagc agagcgccgg ctttctgcgc atccgcggcg gcgacaaccc gctggatgtg
     1921 accggcgtgc accctgaaac ctatccgctg gtcgagcaga tcatcctgaa ggccgggcgg
     1981 ccgatcgagc agctgatggg gcgtgccgag ctgctgcgct cgctcaggcc ggagcagttc
     2041 gtcaccgagg cgttcggcgc cgtcaccgtc agggacatcc tggccgagct ggaaaaaccc
     2101 ggccgcgatc cgcgccccga cttcaaggtg gcgcgcttca acgagggcgt ggaagacatc
     2161 gccgacctga agccaggcat ggtgctggaa ggcaccgtca gcaacgtggc cgccttcggc
     2221 gccttcgtcg acctgggcgt gcaccaggac gggctggtgc atgtgagcca gctggcccac
     2281 aagttcgtgc aggacgcgcg cgaggtggtc aagaccggtc agatcgtgaa ggtcaaggtg
     2341 ctggaggtgg acgcggcgcg ccagcgcatc agcctgacga tgaagctgga cgccgccgcg
     2401 ccgcgccgtg acgcgccgcg cgacaaccgc ttcgagcccg ccgggcggcc ggcgcgcccg
     2461 ggccacgcgg cagggcgcga acgcggctcg gcggccgcca ccgccatggc ctcggcgttc
     2521 gagaaaccgc gcggccatca agccaaatag ccttgtagcg cgcttgcagc atgcgccagc
     2581 agctatcgaa atggaagtga acgggctcgg gccagggcgg tcaccaaagg catcagggct
     2641 tggttcacac cgcctcaccc cctccagcgc agcatgcgca gaccgttgaa caccaccagc
     2701 agcgacgccc ccatgtcggc gaacaccgcc atccacatgg tggcgctgcc cagcaccgcc
     2761 agcagcagaa acaccgcctt gatgcccagc gccagggcga tgttctgcca caggatggcg
     2821 tgcgtgcggc gcgacaggcg cacggtttcg ggcacgcggc gcaggtcgtc gttcatgatc
     2881 accacgtcgg cggcctcgct ggccacgtgc gtgccgccct gacccatggc aaagcccagc
     2941 gccgcggcgg ccagcgccgg ggcgtcgttg atgccgtcgc ccaccatgcc cacggtgcca
     3001 gggctgtgcg cgttcagcgc cgtgatggcc tccagcttgt gttgcggcag caggccggcg
     3061 cgcacgtcgc cgatgccggc ctgagcggcg atggcctgcg cggtggtcgg gttgtcgccg
     3121 gtcagcatga tcggctccac ccccagcgcg cgcagctgct gcacggcggc gggcgtgtcg
     3181 ggcttgagtg cgtcggccac ggcgaacagc gccagcacgc cacggtcgtc ggccagcagg
     3241 ctgacgctgc ggcccagcgc ctcgtgcgcc tgcatgcggg cttccagcgc gggcgaacac
     3301 tggccgcgct cctcgatcca gcggtggttg cccagcacca gcgcctgatc gtggatgcga
     3361 ccgttgctgc cacgcccggc ggcggcgccg aagtcgtgca cggcgagcgg ttcgttgccc
     3421 aggccctgcg cgatggcgcg cgacaccggg tggtcggagc gcgccgccag gctggcggcc
     3481 agctgccaga cgcgggcttc ttcctgccgg tcatcgacca gctcgcgcgc caccaggcgc
     3541 ggccgccctt cggtcagggt gccggtcttg tccagcgcga tgtggcgcag ccgacgcgct
     3601 tcttccaggt acgcgccgcc cttgatcaga atgccgcgcc gtgccgccgc tgccagaccg
     3661 ctgaccaccg tcaccggcgt ggcgatgacc agggcgcagg ggcaggcgat gaccagcagc
     3721 accagcgcct tgtaaagcgc cgtcagccac ggccagccgg ccagccacgg cccgagcagc
     3781 gccaccgcca gcgcgatggc gaacaccgtc ggggtataga cggcggcaaa gcggtccacc
     3841 aggcgctgcg tcggcgcgcg cgtgccctgg gcctgctcga ccgcgtggat gatgcgcgcc
     3901 agcaccgtgt cgctggccgg cgcggtgact tcgtattcca gcgcaccgtg ctggttgatg
     3961 gtgccggcaa acacctcgtc gccgggcgcc ttctcgaccg gcacgctttc gccggtgatg
     4021 ggggcctgat cgatggcgct gtggccggcg gtcacgcgcc cgtccagcgc cagccgctcg
     4081 cccgggcgca cgcgcaccag cgcaccgctc gccacgctgt cggcgcgctg cgtcagccag
     4141 ccgccgtccg gctggcgcac ctcagccagc ggcggcgtca gcgccagcag gccgccgatg
     4201 gcgtggcgcg cgcgatcgac ggcgcgcgcc tcgatcagct cggcgatgga atacagcgcc
     4261 atcaccatgg cggcttcggt ccactggccg atcaggaagg cgccggtcac ggccaccgtc
     4321 atcagggccg aaatgcccag ctggccgcgc gccagcgccg ccaacccctt gcggtagaca
     4381 ccaaagcccg acagcgcaat ggccagcgcc gccagcgcca tgctggcgaa ctgccagggc
     4441 cgggtgtcgg gggcgacgaa gtgcagcagc tcggcgccca gggccagcag cagggcggcg
     4501 gccagctggg tggcctggcg gcgctggcgg cggcgcgtgt cggcggcgct ggtcggctgg
     4561 ctcaggcgct cggttttcag gccggcggcg gcgatggctt gctcgacggc cgaccaggcg
     4621 ctgtcgggcg cgtccaccgc cagcgtgcgc gcgcccaggt cgaagcgcag gccgcgcacc
     4681 gcggcctggc cctccagcgc gcggcgaatc tgcgcctctt cgctggcgca gtccatgtcg
     4741 ggaatgcgca gcagcaaggc gccgcccgcc gccagcgccg agaagtcgat cggccgcgcc
     4801 gccacttcac aggcggcgcc gccgcaatcg tggtcggcgt ggggatggtc gtgcccatgc
     4861 tcatgcgcgt ggtcgtgcgc gtggcggtgc gggtggacgt gggggtgggc ggtgctgctg
     4921 ctcatggggc gattcggtgg cggtctggcg aaccttcggg tatattgaaa acgctgtagt
     4981 cactacagag tcaagcattg cgttgtctca accccacccc aggtgcgtga tcatgaaaat
     5041 cggcgatctg gcccaggctt cggccaccgg cgtggaaacc atccgttact acgagcgtca
     5101 gggcctgctg cccgcgccgg cgcgcacggc gggcaacttc cgcgtctacg acgaggcgca
     5161 cctggagcgc ctgcgcttca ttcgccagtg ccgcggcctg gacatgtcgc tggacgaggt
     5221 gcgcgagctg ctggcggtgc gcgatgcgcc ggacgccgac tgcggcgccg ccaaccgggt
     5281 gttggacgag cacatcggcc acgtcagccg ccgcatccgc gagctgcgtg cgctggagcg
     5341 ccagctcaag gcgctgcgcg ccagctgcgc ggcggtgcag ccgtcgggtg actgcggcat
     5401 tctggtcggg ctgtcgtccg cgctcgaacg gcccgtcgac gcgccgctgc gcagcgcgcg
     5461 ccacctgggc gcggtgcatg gtcagggcgg gggctgatcg cccgcctgtt acagaatgat
     5521 atcgggcgcc ggcgcttacc aatcgtgcgc cagcagctat ttatttgata gcttttggcg
     5581 ccacccgaag ttacatcgag gcgtgcagca tgcgccggta gtcgtcggcg ctggcgatgc
     5641 gcgggttggt cttgtggcaa tggtccagca gggcgtgcgc gatcacgccg tcgaactggt
     5701 tttggctcac gcccatggcg gctaggccgc tgggcaggcc caggcgcgcc gtcatggcgc
     5761 ggacggcgtc ggccacgtcg gccccgggcg gcagccccat ggcctgcgcc atgcgcgcca
     5821 ggcgctgttc gcggcgcatc gattcggcct cggcgttgaa gccgatcacg gccggcagaa
     5881 acaccgcgtt cagcgtgccg tggtgcaggc gcgggtcggc cccgcccagg ctgtggctca
     5941 ggctgtgcac gcagcccagc cctttctgaa agcccatggc gccatggatg ctggcggcca
     6001 tcatgttcag gcgcgcctcg cggtcgctgc cgtcgtgcgt ggcacgctcg atgtgggccc
     6061 agccgcggcg caggccttcc agcgcgatgc cgtcggccgg cgagttgaag gccggtgcca
     6121 tgaacacttc catgcagtgg gcgatggcgt ccatgccagt ggcggcggtc agcttgggcg
     6181 gcaggcccag ggtgagggcc gggtcgcaga tggcggcgcg cggcatcagg tgccaggagt
     6241 gaaagcccag cttgcgcccg tcgtcgacga tcacgatggc gccgcgcgcc acctcgctgc
     6301 cggtgccggc ggtggtgggc acggcgatca gcggcgcggc ggcggcgctg atgcgctcgg
     6361 agccgccctc gatggtggcg taatgggtca gcgggcccgg gtgggtggcg gcgatggcgg
     6421 cgcccttggc gcagtcgatc gggctgccgc cgccgaccgc gatcaggccg tcgcagccgt
     6481 gctgctggta ctgcgccacg gcgccacgca cggcggcctc ggtggggttg gaaggcacgt
     6541 cgtcgaacac cgccacggcg cgcgggccca gtgcgtccag caccggctgc agcacgccgg
     6601 cggcgcgcac gccggcgtcg gtcaccacca gggcgcgcgt gatgcccacg cgctggcaat
     6661 gctcaccgat gcgcgccagg gcgccgaaat cgaactcgat ctgggtcagg tattggatga
     6721 gggccatggg gtgatttcca agctacgatg tatcggcaac tcgattcatt ttcacccaaa
     6781 ccccgaggag gatcccccca tgaagtcacg cacgctacac agcctggccc tggccgccgc
     6841 actggccctg ctggccggtt gcgccagcac gtccgccccg gtggtggccg acacgcccgg
     6901 ctgcctgagc aacgttgccg tcgatcgcct gctggccgac tacaacgcgc gccgcccggc
     6961 ggccaacccg cccgaaaccc tgaccatggc cgatgcgcac tgctcgcgct ccaagctgca
     7021 gcagcgcctg gcggggcagg ccgggcgtct ggtgggctac aaggcggggc tgaccaaccc
     7081 ggcggtacaa aagcgcttca acaccgacca gccggtgtgg ggcgcgctgt acagcggcat
     7141 gatcctcaac agcgacgtca cgctcgacgc cagcttcggc tcgcgcccgc tgtacgaggc
     7201 cgacctgctg gtgcgcgtca aggaccgcgc catcaacgac gccagcacgc cggcgcaggt
     7261 gctggcgcat gtggatcagg tgattccctt catcgaactg cccgacctga tggtcgaggc
     7321 gccacccaag atcaacggcg cggtgctgag cgcactcaac gtcggcaccc gcctgggcgt
     7381 gcgcggcgcg ccgctgctgg tgccggccga cgccgccgga caggcgcgcc tgctcgacca
     7441 gctgcgcgac atgaacgtgc gcctggtcga cggccagggc gcggccctgg gcggcggcaa
     7501 gggcagcgac gtgctgggcc acccgctcaa cgccgtggtg tggctggcca aggcgctgaa
     7561 ggcgcagggg ctgtcgctgc agccgggaca ggtggtcagc ctgggctcgt tctcggccct
     7621 gctgccgccc aagccggggc tgaaggtgac ggtgcactac gacggcgtgc cgggcctgca
     7681 gccggccacg gtcaacttca ggtgaggcgg cggacatccg ttcggcctgg ccgtgcgttg
     7741 atgtccgcgt aaccacgctt tttgtggagg gaactcagat catgtttcga tgcttcaaag
     7801 gtattgccct ggcatgggcg gccctggcgc tgacgccggc ggcccaggcc caatggcagc
     7861 cggtaggccc ggcgctgccg ccgggcggcg gccagatcgt tcagtgcgaa agcctggacg
     7921 gtgatttccg cgaatgccgc accccattcg gggggccgcc catgctggtc aaccagctgt
     7981 cggcctccaa ctgtgtggaa ggccagacct ggggcagccg tgcgccgggc acggtgtggg
     8041 tggcgcgcgg ttgccgcggg cagtttgccg ccgcctacgt gggccagcct ccgggcagca
     8101 tcgtggtgcc tgggggcggc ggcagctacg acgaacgcag cgccgtgcgc tgcgagagcg
     8161 atgacggccg ctaccgcgag tgccgggcgc cggtgggcgg gcgcctgagc ctgctgcgcc
     8221 aggaatcgca gacgccgtgc atcgaaggcc agacctgggg caaccgcgga tcgggcacgg
     8281 tgtgggtcaa cgccggctgc cgcggggtgt ttggcgtggc cggctggggt ggcggcggtg
     8341 gcagcagcga gacctaccgc tgcgaaagcc gcgacgaccg ctacaacgag tgccggggac
     8401 cgacgcgggg tcgccaggtg ctggtgcgcc agttgtccga atcggcctgc atcgagggcc
     8461 gcagctgggg cagccgcaat ggcggcgtct gggtgcagta cggctgccgg ggcgaattcg
     8521 ccgcggcggg cggctggggc ggtggtggca actggggcca gggtggcggc cgctacgaag
     8581 tcgaatgcac cagcgaggac cggcgctaca cccagtgcaa ctgggattcg cgccagggtc
     8641 gcccgtatgt ggtcgagcag tactccagtg acgcctgcat cgaaggctcc agctggggct
     8701 acgaccgccg ccggggcaac ctgtgggtgg atcgcggctg ccgcgctcgt tttggcgccc
     8761 gctgaagccc aacccgattg ctattgttgt gatagcttct ggcgcttgct ggaattgcgc
     8821 cagaggctga tttgaccttg aaccacggcc cagacatcca cgcccgcctg acgcccgcca
     8881 tgcgcctgct ggtcgagcgc atggcgcgtg ccccgtaccc gcccatgcac acgctgacgg
     8941 cggccgaagc caaggcggcc cacgcggcgt cgatcggcgt gctggacgtg cccaaacccc
     9001 ggctgccccg ggtgcagcag ctgcagattc cggcgcgtga tggggcggcg ctgccggcgc
     9061 gcctggtggc gcccagcctt gccgccggcc tgccggtgct gctgtacctg cgcggcggtg
     9121 gcttcaccat cggcagcgtc gagtcgcacg acaccgtctg ccgcgtgctg gccgagcaaa
     9181 gcggtgcggc ggtgctgtcg ctggactatc ggctggcgcc cgagcaccgc tttcccaccg
     9241 cggtgcacga cgcctgggac gcgttgcgct ggctggcggg cgagggcgcg gcggcgctgg
     9301 cgctggatgg cgcgcgcatc gccgtcggcg gcgattcggc cggcggcacg ctggccgccg
     9361 tcagcgccat ccacgcgcgc gacctgggct tgccgctggc gctgcaactg ctcatttacc
     9421 ccggcaccac cgaccaccag gacaccgatt cgcacgccct gtacgcccag ggcccggtgc
     9481 tggacaaggc gctgatcgac tggtttttcc accactacat cgacagcgtc gaccgcagcg
     9541 actggcgctt tgccccgctc aagagcgccg aggtggacgg cgtggccccg gcctgggtcg
     9601 gcctggccga atgcgacccg ctggtggacg aagggctgct gtacgccgac aaactgcgcg
     9661 ccgccggcgt gccggtggag ctggagatct accgcggcgt ggtgcacggc ttcatgacca
     9721 tgggccgcag catcgtcgag gcgcgccagc tgcacgccga cgcggcggcg gcgctgcggc
     9781 gggcgttcga gcgctgagcg ccgcctcggg tactgcaaaa aatatagctg cctgcgcaca
     9841 ccagaagcgc gccaaagcaa gatttgaccc ttcgttctgg ccatgaaacc cgccgacttt
     9901 cgctttcacc acccgctgcg cgtgcgctgg gccgaggtcg atctgcagca gatcgtcttc
     9961 aacccgcact acctgaccta ctacgactgc gccatggccg actactggcg cgcgctggcc
    10021 ttgccttacg aggccacgct gcggcgcttg cagggtgaca tctttctgcg caagaccgcg
    10081 gtcgagttca acgcttccgc gcagctggac gaccggctcg acgtggcgct gcgctgcgag
    10141 cgcgtgggcc attcgtccat caccttcacg ggcgccatct tccgcgacca gcggctgctg
    10201 aacacggccg agctggtgta cgtgtttgcc gatcccgcca gccagcgcag ccgggcggtg
    10261 ccggcgccgc tgcgcgcgct gtttgccgcc tacgaggccg gcgagcgcct gaccgacgtg
    10321 cagctggggc cctggtcgca gctggccgaa cccgcgcgcg ccgtgcgcag cgcggtgttc
    10381 atcgaagagc agggcattgc ccacgacgag gaatgggacg cgctggacgc cgatgccgtg
    10441 cacgcggtgg tgttcaaccg gctgggcgtg ccggtgggca ccggccgtct gctgggtgag
    10501 ccgcaggccg gcacggcgcg catcggccgc atggcggtgg atcgcgcgct gcgcggcggc
    10561 ggcgtcggcc gcctgttgat cgaggcgctg cagcaggccg ccgccgggcg cggcgaccgg
    10621 cgcgtggtgc tgagcgcgca gcgcagcgcc gagggcttct accggcggct cggctatgcg
    10681 ccagtgggcc agccctacga cgaagtcggc atcccccaca tcggcatgca acgcgacctg
    10741 gtctgacccc gcagcgccgc gccggccgtc agacctcgac caccagccgg gggtcgaagc
    10801 gggcgttttc cgtcacgccg tccttgacgt agccgcgcgg attgcacagc acgcgcgtgc
    10861 cgttgacccg gtagtcgtgg ctgtcgtggg tgtggccgtg cacccacagg gcggcgcgct
    10921 ggccgtccag caggtgttcg gcggccgaca cgaaggcggc gttgaagggt gatccggcaa
    10981 agcgcgggtg gatgctgccg gtggagggcg cgtggtgggt gatgaccacc gtcggtccgg
    11041 cgaagggctg ggccagctgc gcagccagcc agagcgtgtt gtgctcgaac aggcgcacgg
    11101 tgtcttgcgg cgtcagcatc ggcgcatcgg ccgcagcggc cacgcgcacg cgcctgaagt
    11161 cgtacatgaa cgcggccgat ctggccatgg cctcggcctg tcgctcgccc tcgccgtggg
    11221 ccctgaagtc ggtccacagg gtcgagccca gaaagcgcac gccgcccagc accagggcgt
    11281 cgttgtcgag cagatggacg ggcgtgccgg cacacaggcg gcgcagctcg gcgcgcacct
    11341 cgggcaggct ggagccgtag aactcgtggt tgccgatgac gtagagcacc ggcttgccaa
    11401 aaccccgcgc ccaggcggcc gccgcggccg ggcgcgcgat gtcgccggcc agcaccacca
    11461 cgtcggcgtc ggtgtgcggg tgcgccaggc cacctgcgct caggtgcagg tcggacagga
    11521 tgtgcagctt catgcgggca gggcgacggc gcgccgggcg tcgggtcggt gggcgattgc
    11581 cggctatcct atcgcgcatc gtgctggcct acatcctcaa gcgtctgctg ctcatgctgc
    11641 ccaccctgct gggggtgctg ctgctcacct tcgtggtgat ccagttcgtg ccgggcgggc
    11701 cggtggagca gtacctggcc gaggccaagg ccggtgtggg tggcgccgcc ggggccgaag
    11761 gcggcggcct ggcctaccgc ggtgcccagg gcgtggaccc caagcggctg gagcagatca
    11821 aggcgctgta cggcttcgat cagccggcgc accagcgctt cgtgcagatg ctggggcgct
    11881 tcgcgcgctt cgacctgggc accagcttct tccagaacaa ggcggtgtgg gcgctgatcc
    11941 gcgaaaagct gccggtgtcg atcagcctgg ggctgtggac cttcttcatc agctacggca
    12001 tcagcgtgcc gctgggcgtg gcaaaggcgg tgcgcgcggg cagccgcttc gacctggcca
    12061 ccacgctgct ggtgctggtg ggctacgcca tcccgggctt cgtgctgggg gtggcgctgc
    12121 tggtgatctt tggcgggcaa ctgcaatggt ttccgctgcg cgggctgacg tcgccgaact
    12181 tcgacgaact gagcctgggc ggcaagatcg tcgactacct gtggcacatc acgctgcccg
    12241 tcaccagcat ggtgctgggc agctttgccg tcaccgccat gctgaccaag aattcgtttc
    12301 tggaggaaat ccgcaagcag tacgtgctga cggcgcgcgc caagggcctg accgagcggc
    12361 aggtgctgtg gaagcacgtg ttccgcaatg cgctgattcc catcatcacc ggctttccgg
    12421 ccgccttcat cggcgctttt ttcaccggtt cgctgctgat cgagaccctg ttttcgctcg
    12481 acggcctggg cctgctgagc tacgaaagcg tgatccggcg cgactacccg gtggtgctgg
    12541 gcacgctgta cctgttcacc ctgatcggcc ttgtgaccaa gctgatcagt gacctgacct
    12601 acgtgtgggt ggatccgcgt gtcaagttcg actgagggca tggtcaccgc atcggtttcg
    12661 ccggcgcgcc gcgcctggcg gcgctttcgg cgcaaccgcc tgggcttgtg gagcctggtg
    12721 ctgtttggtg tcctggtggg gttcagcctg ctggccgagc tggtgtccaa cgacaagccg
    12781 ctgctggtgc gctaccaggg gcagtggtac gtgccactgg tcaagaccta cccggaaaag
    12841 gtgttcggcg gcgacttcga gaccgagacc gactacctcg accccttcat ccgccagcag
    12901 ttcagtcagc ccggcaactg ggccatctac ccgcccaacc cgtatggcgc cagaaccatc
    12961 aactactttg ccaaggcacc caaccccagc gcccccagcc gcgacaactg gctgggcacc
    13021 gacgaccgcg ggcgcgatct gctggcgcag ctcatctacg gctttcgcgt cagcgtgctg
    13081 ttcgccctgg cgctgacggc ggtcggcgtg ctgctgggcg tggcggcggg ggcggtgcag
    13141 ggctttttcg gcggcaggat cgatctggcg tttcagcgct tcatcgagat ctggggctcc
    13201 atgcccgagc tgtacctgct
//
LOCUS       DAOQTZ010000080        12987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5410_length_12987_cov_14.656588, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000080 DAOQTZ010000000
VERSION     DAOQTZ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12987
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5410_length_12987_cov_14.656588"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..1121)
                     /locus_tag="PKN51_09865"
     CDS             complement(348..1121)
                     /locus_tag="PKN51_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNT85446.1"
                     /translation="MKLTDEILFEVEDGGIAVITIDRPQARNAINSGVIEGLRLAWQR
                     FAGDAALRVAILTGSGDKAFCAGMDLKQAAATGLRIPPRDMFPILGDAVKLDKPVIAA
                     VNGVAYAGGFLFAQMCDLCVASDNALFGITEAKVGRGMPWAAPLAQMLPQRIMMELLL
                     TGAPMTAARAHQLGYVNAVVPLAELRDTALGMARTIAANAPLTVRAARELVYLSSEMG
                     RSAALRAAYPLFDSVYLSEDAQEGPRAFAEKRAPRWSGR"
     gene            complement(1118..2386)
                     /locus_tag="PKN51_09870"
     CDS             complement(1118..2386)
                     /locus_tag="PKN51_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNT85447.1"
                     /translation="MQLESLDSLDLARHVRPGDGVIFQQGCGEALSLAERFVQQRASY
                     SGARLFLGSGFSRSFQPEHADHLRFSGIGGIGSLRKLASAGALDPIPCHISSIEPLLR
                     QGVIASDVVMLQVSPPNERGEYSLGLVNDYVRTAIGRARVVIAEINRQIPWVHCDLPL
                     TAADISVAVETDRAPLELPPAPFGALEQRIAQHIGDLIGDRSTLQMGIGSVPEAIMSS
                     LADRRELGIHSGMIGDSAVTLMECGAVTNAHKGMDAGVTVSGVLFGTQKLYRYAERNP
                     QIRLHPTSYTHHIGVLARLKNFVSINSALEVDLTGQINAEAIGREHIGAVGGQVDYIR
                     AAALSEGGVSIVALPATGKGGDSKIVSRLSGPVTTARSDVDVVATEHGVAHLRGRSLR
                     QRVRALVAIAAPEHRERLLAEAREVLGENV"
     gene            complement(2386..3249)
                     /locus_tag="PKN51_09875"
     CDS             complement(2386..3249)
                     /locus_tag="PKN51_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNT85448.1"
                     /translation="MIRSLFFAPANRPELVLKFPRFGADCCVIDLEDGTPPGEKRSAR
                     EALAQTVARVRAAGLEGTLMVRVNVPDSEHYLADLEAAFASDIDGVVVPKVETTEQVF
                     PVLHWIRQRDAQAPRAQPRALIGGIESVRGVLNAVALCRDIDALSAVFFGAEDFAADI
                     GGRRTPRGDEVYTARSMVVMAARAAGRIAIDQAVIDIRNDALFLDDAGRGRDLGYEGK
                     ICVTPGQVKLAHRAFSPTPEERNYAQRLVETYEAAMARGLGTIDFEGRMIDGPTLKRA
                     QQVLALPAQEA"
     gene            complement(3265..4062)
                     /locus_tag="PKN51_09880"
     CDS             complement(3265..4062)
                     /locus_tag="PKN51_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNT85449.1"
                     /translation="MTPFLLYQQDGPVVTLTLNAPDTRNVLTGNSAPDEFSEACARIN
                     GDASVRAVILTGAGAAFSAGGNLKDMQQMFDWSPDRLRNWYRTGVQRLALAVYHLEVP
                     VICAVNGPAIGAGCDLTTMCDIRIASEQATFAESFIRVGLLPGDGGAWLLPRVVGKAV
                     AAELTFTGRTVDAREALAIGLVSRVVAHEQLLAEARALAEQIAAQPGVTLRMAKKLLR
                     AGERLDFAPLLEMSAGLQALAHKTPHHREAVDAFVQKRPPDFTPFQD"
     gene            complement(4148..5167)
                     /gene="dctP"
                     /locus_tag="PKN51_09885"
     CDS             complement(4148..5167)
                     /gene="dctP"
                     /locus_tag="PKN51_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNT85450.1"
                     /translation="MTKLLPITLAVAASLCAAGHAAAQTKIKVADSFPVGHYLPRYFT
                     TPMMERLKADPAAKGIEFEYYPAEQLGKAKDLLSLTQSGLVDVAYVGPGFVSDKMPLS
                     VVAELPLPYTGSCQATLAYWNLAKPGGLLDKKEFAPNGVRLLFTIVLPPYQVITRKPF
                     ASLKDLEGMKIRASGSAKELLLKELKAVPVLMPTPEVYESLSRGTIDGVLFPFNSLAP
                     YGIDKLSKSGTIGSNFGSYVANWVISEKRYQSLPPALQKDLAAMGDELTRSVCKQVER
                     DEAGDVDKTRAAGVQLKPLSPADQQALEQVAAQVATDWAQKLDRRGKSGTEVLNAFKA
                     GLQAK"
     gene            complement(5196..6485)
                     /locus_tag="PKN51_09890"
     CDS             complement(5196..6485)
                     /locus_tag="PKN51_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNT85451.1"
                     /translation="MTVALIMLALFILLAIGMPVAFALAFAGTLGLYLVGGWQALSGV
                     LATAPLSSVESYELISVPLFILMAEFVILSGVADRLFAAAATWVGRVPGGLAMATALA
                     GAGFGAISGSSTASAATLSSTTLPAMLKQGYEARLSGGVVAISGTLAMLIPPSIALIL
                     YGIIADVSIGALLIGGVIPGILVTLVIIATVWVLVLINPAAAPKGRAYTVREKLASLR
                     VVGPMLFLFMAVTGVIYTGIATPTEASGLGAFGAFLIVLWERRLTRAGLSKALVHAAH
                     ASCMIVMIIVCAKIFGYFFTLTQSTQALVAWVGALDVSRWVILALILLGYLILGCLMD
                     QVAILILTVPIVLPVIKALGYDPVWFGVIVILMAEVGLVTPPVGLNAFVVARYAKRPL
                     AEIFGGVWPHVISHLLLVAVLVAFPQIILWLPSKMGG"
     gene            complement(6482..7042)
                     /locus_tag="PKN51_09895"
     CDS             complement(6482..7042)
                     /locus_tag="PKN51_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT85452.1"
                     /translation="MAPAWYRHLSRAVDVIDNVLVSLGCGLLFALMCLVVADVARRYL
                     FNAPIAWSYEVINHYVMPGVFFYTVAHTLKAHAHVSVDILHNYVQRRTRYVFELIGSV
                     LALPVFVLATVLAAGKTLEDFETAAQASSGLAVPTWTIGMVVPIGFGMLSLRLALNVI
                     GYVSTLATGRAIKALPPISGTEEGAE"
     gene            complement(7107..7562)
                     /locus_tag="PKN51_09900"
     CDS             complement(7107..7562)
                     /locus_tag="PKN51_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNT85453.1"
                     /translation="MAGLYFEEFHVGQQFVHPWSRTVTEMDNTLFSLLTMNPQPLHLD
                     AHFAAQTEWGQRLFNSLYTLGILVGMSVNDTTLGTTVANLGMTDVRFPKPVFHGDTLR
                     ARTEVISVRASQSRPESGLVEFEHTGLNQRDEVVAICRRTALMRRRPSA"
     gene            complement(7571..8710)
                     /locus_tag="PKN51_09905"
     CDS             complement(7571..8710)
                     /locus_tag="PKN51_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNT85454.1"
                     /translation="MNRAGPLQGVRVVEFVGIGPAPLCGMLLADLGAEVLRVERPTPS
                     GLGIERPAQFDLMRRGKRTLRLDLKAAAGLDLARALVRRADALIEGFRPGVMERIGLG
                     PEVCLQDNPALVYGRVTGFGQHGPLSQTAGHDLNYIALTGALDAIGRQGQPPTPPLNL
                     LGDYAGGSLFLALGLLSALLHARSSGEGQVVDAAMIDGVGALMAPFHGLQAAGLHAGP
                     RGTNVLDSGAPYYDVYACADGEFISIAAIEPKFRAELRERLRVAGAAVDDLPDFDDRG
                     RWPELRTRLVALFASQTSAYWCAQLEGSDACFAPVLSVGAAAQHPHHIARASFMVVDG
                     VTQPAPAPRFSATPCGVPSGPTQGGDAKEWALAWGVPAELIAHMD"
     gene            complement(8707..9960)
                     /locus_tag="PKN51_09910"
     CDS             complement(8707..9960)
                     /locus_tag="PKN51_09910"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNT85455.1"
                     /translation="MNSARPRADHQAAQPPLHGIRVIDMSSVLMGPFASQALGDMGAD
                     VIKIEAPAGDVVRQIGPARHADMGPIFLNTNRNKRSVVLDLKHADGVAALKRLLGDAD
                     VLLYNVRPQAMTRLGLDYDSVRALNPRLVYAGVFGFGQDGPYAARPAYDDLIQGAATL
                     PYLMARASGDIPRYVPTAVADRIVGQVAVGAILASLLARQRTGLGDRVDIPMFETMVG
                     FVLGDHLGGLTYDPPLDGGGYARQLSPERRPYRTRDGYICALVYNDKQWAGFLRGIGR
                     STLPQEDPRFASFASRAAHIDHVYGWLAQVFEQRSTQEWFDLLQEADVPCMPMHDLHS
                     VLSDPHLLATGFFQTMEHPSEGTVRSMRLAPRWQTAQIATPRPAPRLGEHTRQVLQES
                     GFSTPEIDRLLHVRAAIAAAPEESA"
     gene            complement(9982..11136)
                     /locus_tag="PKN51_09915"
     CDS             complement(9982..11136)
                     /locus_tag="PKN51_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT85456.1"
                     /translation="MSFHQEHDALRESANRFLIEQCGFERHRQRQQSADAGSNLALWR
                     QMAELGWLGITLGEPWGGMAQSPRAALSLIEAMGAQFVPAPYAEALAATHLLQLLGRP
                     DQQATWLPRLCEGAALGLVAHVEPGARYELDCVSTRADKVEQGFRLSGRKSAVPWGDA
                     ADLLLVSARTGDAPGQRGDLSLFVVERSTAGLRFEGHAGIADERWADVILDHVVVPQA
                     ALLGPAGEAQDALDGTHAFLVAADCAEAVGTMDAVLQETREYASTRKQFGTPLARFQV
                     IAHRLVDMFIQVQLARSMAQLAASIVDDGPNTSAARLRLAACKAQISHACRFVGEQAV
                     QIHGGIGMTDELALSHRVRRLLAIDRRRGDQFHHLGTLAESVQQGVGLYA"
     gene            complement(11153..12313)
                     /locus_tag="PKN51_09920"
     CDS             complement(11153..12313)
                     /locus_tag="PKN51_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT85457.1"
                     /translation="MHAHDLDADRALREEVRAFLREALPDTLRAKVLGHAPLTKDDYV
                     SWQRILNARGWLAPAWPVARGGTGWTPRQIYIFEEECWLAGAPEVFAFGPKMLAPVLM
                     QHGTPAQQDEFLPRILSSEDWWCQGYSEPGAGSDLASLRTRGRIEGDTLVIDGQKTWT
                     TYAQYANRMFCLVRTDPQAKSQEGISFVLLDMDMPGITVRPLILLDGGHEVNEVFLDQ
                     VRVPLSRVVGPLNQGWTVAKALLGHERCNVGKIGRSLRELRLLKRLAAQPDALGRSLL
                     QDVRFAERIAAVEVDLLALEQTTLRLIAEVQAGRSVGAEASTLKIRGTEIAQQLSLLL
                     VQACGDCTAPMGDGLPTPMALVRQYLNWRKLSIYGGSNEVQHNIIAKFALGI"
     gene            complement(12320..>12987)
                     /locus_tag="PKN51_09925"
     CDS             complement(12320..>12987)
                     /locus_tag="PKN51_09925"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT85458.1"
                     /translation="MKKLTYDPIKELRVIAIVGELPTVAVTNLQTPATDLRSFAAYAK
                     ANDGKLNFASLGQGNVLYLTARKIEGELGIRMTEVPYKGSAPSLTALMANDVQFYIDV
                     LPGALPFIQSGKLKALAVPMSKRISWLPDVPTLEEAGYGAFHAASWLGWAVPAATPPD
                     VVARLQGAIGRFAQDEKFRATFTKAGVLMLPAMTPAQVDEYLKADRERWGALIRQHNI
                     TID"
BASE COUNT         2262 a         4406 c         4289 g         2030 t
ORIGIN      
        1 cgccggcgac atgcgtcgcg gccagagcct ggtcgtctgg gccatccgcg aaggcaggca
       61 agcggcgcgc gcgatcgacg aagccctgat gggccgcagc acgctgccgc gttgatccgg
      121 ccggtctgag ccggaccggc gagcccatcg tacgtcaggt ctcggttccg ctgcgccgga
      181 gcacgcccac cgcgaggcgg gcgatcgcca gacagctggt cagaccggac gattcgatgt
      241 ccaacacgcc aggcggccgt ccatgatcaa ctcgaccacc tagtcagggt agtaccagaa
      301 ggtgaggatc ggcgcctcga tgccgaacct ctggcgctgg cggttgttca tcgcccgctc
      361 cagcgcgggg cgcgcttttc ggcaaaggcc cgcgggcctt cctgtgcgtc ctcgctcagg
      421 tacacgctgt cgaacagcgg atacgccgca cgcagcgcgg ccgagcggcc catctcgctg
      481 gacaggtaga ccagctcgcg cgccgcgcgc accgtcagcg gcgcgttggc ggcgatggtg
      541 cgcgccatgc ccagggcggt gtcgcgcaac tcggccagcg gcaccacggc gttgacgtaa
      601 cccagctggt gcgcgcgcgc ggcggtcatc ggcgcgccgg tcagcagcag ctccatcatg
      661 atgcgctgcg gcagcatctg cgccagcggc gccgcccagg gcatgccgcg gccgaccttg
      721 gcctcggtga tgccgaacag cgcgttgtcg ctggccacgc acagatcgca catctgcgca
      781 aacagaaagc cgcccgcgta ggccacgccg ttgacggcgg cgatcaccgg cttgtccagc
      841 ttgaccgcgt cgcccaggat cgggaacatg tcgcgcggcg gaatgcgcag gcccgtggcc
      901 gccgcctgct tcaagtccat gccggcgcaa aaggccttgt ctccactgcc ggtgaggatg
      961 gccacgcgca gcgccgcgtc gcctgcaaag cgctgccagg ccaggcgcag gccctcgatg
     1021 acgccgctgt tgatggcgtt gcgcgcctgc gggcggtcga tggtgatgac ggcgatgccg
     1081 ccgtcttcga cctcgaacag gatttcgtcg gtcagcttca tacgttttct cccagaacct
     1141 cgcgtgcctc ggccagcagg cgctcgcgat gctccggcgc cgcaatggca accagcgccc
     1201 gcacgcgctg gcgcaggctg cgcccgcgca gatgcgccac gccgtgctcg gtggccacca
     1261 catcgacgtc gctgcgcgcg gtggtcacgg ggccggacag gcggctgacg atcttcgagt
     1321 cgccgccttt tcccgtggcc ggcaaggcca cgatggagac gccgccttcg gacagcgcgg
     1381 cggcgcggat gtagtcgacc tgcccaccga cggcgccaat gtgctcgcgc ccaatggcct
     1441 cggcgttgat ctggcccgtc aggtccacct ccagcgccga attgatcgag acgaagttct
     1501 tgagccgcgc cagcacgccg atgtggtgcg tgtagctggt cgggtgcagc cggatctgcg
     1561 ggttgcgctc ggcatagcgg tagagctttt gcgtgccgaa cagcacgccg ctgacggtga
     1621 cgcccgcatc catgcccttg tgcgcgttgg tgacggcgcc gcactccatc agcgtcacgg
     1681 ccgagtcgcc gatcatgccc gaatggatgc ccagctcgcg ccggtcggcc agcgagctca
     1741 tgatggcctc gggcacgctg ccaatgccca tctgcagcgt ggagcgatcg ccgatcagat
     1801 cgccgatgtg ctgcgcgatg cgctgctcca gcgcgccgaa gggcgcgggc ggcagctcca
     1861 gcggcgcgcg gtcggtttcc accgccacgc tgatgtcggc cgccgtgagc ggcaggtcgc
     1921 aatgcaccca ggggatctgg cggttgatct cggcaatcac cacgcgcgcc cggccgatgg
     1981 cggtgcgcac gtagtcgttg accaggccca ggctgtattc gccgcgctcg ttgggggggc
     2041 tgacctgcag catcaccacg tccgaggcga tcacgccctg gcgcagcagc ggctcgatcg
     2101 aggagatgtg gcagggaatg ggatcgagtg cgccggcgct ggcgagcttg cgcaggctgc
     2161 cgatgccgcc gatgccggaa aagcgcaggt ggtcggcgtg ctcgggctgg aagctgcgcg
     2221 aaaagcccga gcccagaaac agccgtgcac cgctgtagct ggcgcgctgc tgcacgaagc
     2281 gctcggccag gctgagcgcc tcgccgcagc cctgctggaa gatcacgccg tcgcccggcc
     2341 gcacatgacg cgccagatcc aggctatcga gggattcgag ctgcatcacg cctcctgcgc
     2401 cggcagggcc agcacctgct gcgcgcgctt caaggtgggg ccgtcgatca tgcggccctc
     2461 gaagtcgatc gtgcccagcc cccgcgccat ggcggcttcg taggtctcaa ccaggcgctg
     2521 cgcgtagttc cgctcttcgg gtgtgggcga aaacgcgcgg tgcgccagct tgacctggcc
     2581 gggcgtcaca cagatcttgc cctcgtagcc caggtcacgg ccgcggccgg cgtcgtccag
     2641 aaacagcgcg tcgttgcgga tgtcgatcac cgcctggtcg atggcaatgc gcccggccgc
     2701 ccgggctgcc atcaccacca tggagcgggc ggtgtagacc tcgtcgccgc gcggcgtgcg
     2761 gcgcccgccg atgtcggcag cgaagtcctc ggcgccaaaa aacaccgcgc tcagtgcatc
     2821 gatatcgcga cacagcgcca cggcgttgag cacgccgcgc accgactcga tgccgccgat
     2881 cagcgcgcgc ggctgcgcgc gcggcgcctg ggcgtcgcgc tggcgtatcc agtgcaagac
     2941 cgggaagacc tgctcggtcg tctccacctt ggggacgacg acaccgtcga tatcgctggc
     3001 aaaggccgcc tccaggtcgg ccagatagtg ctcggagtcg ggcacgttga cgcgcaccat
     3061 cagcgtgccc tccaggccgg ctgcgcgcac ccgggccacg gtctgcgcca acgcctcgcg
     3121 ggcgctgcgc ttttcgcccg ggggcgtgcc gtcttccagg tcgatgacgc agcagtcggc
     3181 gccaaagcgc gggaacttga gcaccagctc gggccggttg gcgggggcaa agaacaggga
     3241 tcggatcatg gtgtctggct ggggtcagtc ctgaaagggg gtgaagtcgg gtgggcgctt
     3301 ttgcacgaag gcatcgaccg cctccctgtg gtggggcgtc ttgtgcgcca gtgcctgcag
     3361 cccggccgac atctcaagca agggggcgaa atcgagccgc tcgcccgcgc gcagcagctt
     3421 cttggccatg cgcagcgtca cgccgggttg ggcggcgatc tgctcggcca gggcgcgggc
     3481 ctcggccagc aactgctcat gcgcgaccac gcgcgagacc aggccgatgg ccagcgcctc
     3541 gcgcgcatcc accgtgcggc cggtgaaggt cagctcggca gccaccgcct tgcccaccac
     3601 ccggggcaag agccaggcgc cgccgtcgcc gggcaacagc ccgacgcgaa taaagctttc
     3661 ggcgaaggtg gcctgctccg aggcgatgcg gatgtcacac atcgtcgtca gatcgcagcc
     3721 ggcgccgatg gccgggccgt tgacggcgca gatcaccggc acctccaggt ggtagaccgc
     3781 cagcgccagt cgctgcactc cggtgcggta ccagttgcgc agccgatcgg gcgaccagtc
     3841 gaacatctgc tgcatgtcct tcaagtttcc gccggcggaa aacgccgcgc ccgccccggt
     3901 caggatgacg gcacgcaccg aggcatcgcc attgatgcgc gcgcaggctt cagaaaactc
     3961 gtccggcgcg ctgttgccgg tcagaacgtt gcgcgtatca ggcgcattga gcgtgagggt
     4021 gacgacgggg ccgtcctgct gatagagaag aaagggcgtc atggaaggcg agttgctgca
     4081 aaggtcgagg tcagcaccgg ctgctcacga ggccggtgct ggcgaaatcg gagaagcgcg
     4141 tcacccgtca cttggcctgc aacccggcct tgaacgcatt cagcacctcg gtaccgctct
     4201 tgccgcggcg gtcgagcttt tgcgcccaat ccgtggccac ctgggcagcc acctgctcca
     4261 gcgcctgctg gtccgcaggg gacaatggtt tgagctgcac gcctgccgcg cgagtcttgt
     4321 cgacgtcgcc cgcttcatcg cgctcgacct gtttgcatac gctgcgcgtc aactcgtcac
     4381 ccatggctgc cagatccttt tgcagcgcgg gtggcaacga ctggtagcgc ttttcgctga
     4441 tgacccagtt ggccacgtag gagccaaagt tggagccgat cgtgcccgac ttcgagagct
     4501 tgtcgatgcc gtagggggcc aggctgttga acgggaacag cacgccgtca atggtgccgc
     4561 gcgagagcga ttcgtagact tcaggcgtgg gcatcagcac cggcacggcc ttgagctcct
     4621 tgagcagcag ctccttggcc gaccccgacg cgcgaatctt catcccttcc agatccttca
     4681 ggctggcaaa cggcttgcgc gtgatcacct gatagggcgg cagcacgatg gtgaacaaca
     4741 ggcgcacgcc gttgggcgca aattccttct tgtccagcag gccgcccggt ttggccagat
     4801 tccagtaggc cagcgtcgcc tggcaggagc cggtgtaagg cagcggcaac tcggccacca
     4861 ccgaaagcgg catcttgtcc gacacaaagc cggggcccac ataggcgaca tcgaccaacc
     4921 cggactgggt aagcgagagc agatccttgg ccttgcccaa ctgctcggcc gggtagtact
     4981 cgaactcgat gcccttggct gccggatcgg ccttcaggcg ctccatcatg ggggtggtga
     5041 agtaccgcgg caggtagtgc ccgacgggga atgaatccgc gaccttgatc ttggtctgcg
     5101 cggctgcgtg gccggcggca cacagactgg cggcaacggc cagggtgatg ggaagcagct
     5161 tggtcatgtc atgtctcctt tatcgcgttg aaaattcatc cgcccatctt ggagggcagc
     5221 cacaggatga tttgcggaaa cgccaccagc acggccacca acagcaggtg actgatcacg
     5281 tgtggccaga cgccaccgaa aatctcggcc agcggccgct tggcgtagcg ggcaaccaca
     5341 aaggcgttca ggcccacggg cggcgtgacc agaccgacct cggccatcag gatcacgatc
     5401 acgccgaacc acaccgggtc atagcccagc gccttgatca ccggcagcac gatgggaacg
     5461 gtgagaatca ggatggcgac ctgatccatc aggcaaccga ggatcagata gcccagcaaa
     5521 atcaaggcca ggatgaccca gcgggacaca tccagcgccc ccacccacgc caccagcgcc
     5581 tgcgtgctct gtgtgagcgt gaagaagtag ccaaagatct tggcgcagac gatgatcatc
     5641 acgatcatgc agctggcatg ggctgcgtgc accagggcct tggatagacc cgcgcgggtg
     5701 agtcggcgct cccacagcac gatcaggaag gcaccgaacg ctcccagccc cgaagcctcg
     5761 gtgggcgtgg cgatgccggt gtagatgacg ccggtcacgg ccatgaacag gaacagcatc
     5821 ggccccacga cgcgcagcga ggccagcttc tcgcgcaccg tgtaggctcg ccccttgggc
     5881 gccgccgcag ggttgatcag caccagcacc cacacggtgg cgatgatcac cagcgtcacc
     5941 aggatgccgg gaatcacgcc gccgatcagc agcgcgccaa tgctgacgtc ggcaatgatg
     6001 ccgtacagga tcagggcgat cgatggcggg atcagcatgg ccaaggtgcc cgagatggcc
     6061 accacaccac cactcagacg agcctcgtaa ccctgcttga gcatggcagg caaggtggtg
     6121 gacgacagcg tcgcggcgga agccgtgctg gaccctgaaa tcgcaccgaa gccggcgccg
     6181 gccagcgccg tggccatggc caagccgccc ggcacgcggc cgacccaggt agcggccgcg
     6241 gcaaacagcc tgtctgccac accactcaga atgacgaatt cggccatcag gatgaacagc
     6301 ggcaccgata tcaattcgta ggattcgacg ctcgacagcg gagcggttgc cagcacgccc
     6361 gacaaggcct gccagccacc caccagatac aagcccagcg tgccggcaaa ggcaagcgca
     6421 aaagccaccg gcatgccgat ggccagcagg atgaacagcg ccagcatgat cagtgcaacg
     6481 gtcattcggc cccctcctcg gtgcccgaga tgggtggcag cgctttgatg gcgcggccag
     6541 tggccaaggt gctgacgtag ccgatcacgt tcagggccaa ccgcaggctc agcatgccga
     6601 agccgatggg cacgaccatg ccgatggtcc atgtgggcac ggccaggccg ctgctggcct
     6661 gggccgcagt ttcaaaatcc tccagcgtct tgccggcggc cagcacggtg gccagcacga
     6721 acaccggcag tgccaatacc gatccgatca actcgaacac atagcgggta cgacgctgca
     6781 catagttgtg caggatgtcc accgacacgt gggcgtgtgc tttcagcgtg tgggccacgg
     6841 tgtagaagaa cacccccggc atgacgtagt ggttgatgac ctcgtatgac cacgcaatgg
     6901 gcgcgttgaa caggtagcgg cgcgccacat ccgccaccac caggcacatc agggcaaaca
     6961 gcagtccaca gcccaggctc accagcacgt tgtcgatcac gtcgaccgct cgactcagat
     7021 gccggtacca ggcgggcgcc acgggtgctt caggatcacc ttgattcatc ggtcagcctt
     7081 tcgaatcgag agacacaacg gacgctctat gcagacggcc ttctgcgcat cagggccgta
     7141 cgccgacaga tcgcgacgac ttcgtcccgc tgattcaggc cggtgtgctc gaactcgacc
     7201 aggcccgatt cgggccggga ctggctggcg cgcaccgaga tcacctcggt gcgggcgcgc
     7261 agcgtgtcgc catggaacac gggcttggga aagcgcacgt ccgtcatgcc gaggttggcc
     7321 accgtggtgc ccagcgtggt gtcgttgacg ctcatgccca ccaggatgcc cagcgtgtac
     7381 aggctgttga acaggcgttg cccccattcg gtctgagccg cgaagtgggc gtccagatgc
     7441 agcggctgcg gattcatggt cagcagggaa aacagcgtgt tgtccatctc ggtcacggtg
     7501 cgcgaccagg ggtgaacaaa ctgctgaccg acgtgaaatt cctcgaagta gagacctgcc
     7561 atgatgctcc tcagtccatg tgtgcgatca gttccgccgg cacgccccag gccagcgccc
     7621 attccttggc gtcgccgccc tgtgtcggtc cgctgggcac accgcagggg gtggcgctga
     7681 accgcggcgc cggcgcgggt tgcgtcacgc catcgaccac catgaagctg gcgcgcgcga
     7741 tgtgatgggg gtgctgcgcc gcggcgccca ccgaaagcac cggggcaaag caggcgtcgg
     7801 acccttccag ctgcgcacac cagtacgcgc tcgtctggct ggcaaacagg gcgaccaatc
     7861 gggtgcgcag ctcgggccag cgccctcgat cgtcgaaatc cggcaaatcg tcgacagcag
     7921 caccggccac gcgcaggcgc tcgcgcaact cggcgcggaa cttcggctcg atagcggcga
     7981 tcgagatgaa ctcgccatcg gcgcaggcat agacgtcgta gtaaggggcg ccggaatcga
     8041 gcacgttggt cccgcgcggt ccggcatgca gcccagccgc ctgcagaccg tggaacggcg
     8101 ccatcagcgc acccacaccg tcgatcatcg cggcatccac cacctgcccc tcgccgctgc
     8161 tgcgggcgtg caacagagcg gacagcagcc ccagagccag aaacagggaa ccaccggcgt
     8221 agtcccccag caggttcagg ggcggcgtgg gcggctgccc ttgccggccg atagcgtcca
     8281 gcgcgccggt cagggcgatg tagttcagat cgtgcccggc cgtctgcgac agcggcccgt
     8341 gctggccaaa tccggtgacg cggccgtaca ccaacgccgg gttgtcctgc aggcaaacct
     8401 ccggccccaa tccgatgcgt tccatgacac cggggcgaaa accctcgatc agcgcatcgg
     8461 ccctgcgcac cagcgcgcgc gccaggtcca gaccggcggc agccttcaga tcgagccgga
     8521 gggttcgctt gccacgccgc atcagatcga actgcgccgg ccgctcgatc cccaggccgc
     8581 tgggcgttgg ccgctccacc cgcaacacct cggcccccag atcggccagc agcatgccgc
     8641 acagcggcgc cgggccaatg ccgacgaatt cgaccacgcg cacaccttgc agcggccctg
     8701 cccggttcat gcggactcct ccggcgcagc cgcaatggcc gccctcacat gcagcaggcg
     8761 gtcgatttcc ggggtgctga agcccgactc ctgcagtacc tgtcgggtgt gctctcccag
     8821 gcgcggcgcc gggcgcggcg tcgcaatctg ggcggtttgc cagcgcggcg ccaggcgcat
     8881 cgagcgcacc gttccttcgc tgggatgctc catggtctga aagaacccgg tggccagcag
     8941 gtgcggatcg ctcagcacgc tgtgcagatc gtgcatcggc atgcagggca cgtcggcctc
     9001 ctgcagcaag tcaaaccact cctgcgtgct gcgctgctcg aacacctgcg ccagccagcc
     9061 ataaacatgg tcgatgtgcg cggcgcggct ggcaaagctg gcaaagcggg ggtcttcctg
     9121 cggcagcgtg ctgcggccga tgccgcgcag aaatcccgcc cactgcttgt cgttatagac
     9181 cagggcgcag atgtagccat cgcgcgtgcg gtacggccgg cgttcggggg agagctgcct
     9241 ggcatagccg ccaccatcca gcggcgggtc atatgtcagg ccccccagat ggtcgcccag
     9301 cacgaagccg accatggttt cgaacatggg gatgtcaacg cggtcgccca ggccggtgcg
     9361 ctggcgcgcc agcaggctgg ccaggatggc gccaacggcc acctggccga cgatacggtc
     9421 ggccacggca gtggggacgt agcgcggaat gtcgccgctg gcgcgtgcca tcaggtaggg
     9481 cagcgtggcc gcgccctgga tcaggtcgtc ataggcaggg cgcgccgcat aggggccgtc
     9541 ctgaccgaag ccgaacaccc cggcgtagac caaccgggga ttgagcgccc gcacgctgtc
     9601 gtagtccagt cccagccgag tcatggcctg gggccgcacg ttgtagagca gcacgtcggc
     9661 atcgcccagc aatcgcttga gggccgccac gccatcggca tgtttgaggt ccagcaccac
     9721 gctgcgcttg ttgcggttgg tgttgagaaa gatcgggccc atgtccgcgt gacgggccgg
     9781 gccgatctgg cgcaccacgt caccggccgg cgcctcgatc ttgatgacat ccgcacccat
     9841 gtcgcccagc gcctgcgagg caaacggccc catcagcacc gagctcatgt cgatgacccg
     9901 gatgccatgc agcgggggtt gggcggcctg gtgatctgcg cgggggcggg ccgaattcat
     9961 gatgcgttga tccatcctgt ctcaggcata aagccccacg ccttgctgaa cgctctcggc
    10021 cagcgtgcca aggtgatgaa actgatcacc gcgccgccga tcgatggcca gcaggcgacg
    10081 cacccggtgc gacagcgcca gttcgtccgt catgccgata ccgccgtgga tctgcaccgc
    10141 ctgctcgcca acgaaacggc acgcatggct gatctgcgcc ttgcaggctg ccaatcgcag
    10201 gcgtgccgcg gaggtgttcg ggccatcgtc cacgatggac gcagccagct gcgccatgga
    10261 gcgcgccaac tgcacctgaa tgaacatgtc caccaggcga tgggcaatca cctgaaagcg
    10321 cgccagcggc gtgccaaatt gctttcgcgt gctggcgtac tcgcgcgttt cctgcagcac
    10381 ggcatccatg gtgcccacgg cttcggcgca atcggctgcc accaggaacg catgggtgcc
    10441 atcgagcgca tcctgcgcct ccccggcggg gcccagcagc gcggcctgcg gcaccacgac
    10501 gtggtccaga atcacatcgg cccatcgttc atccgcgatt ccggcgtggc cctcgaagcg
    10561 caggcccgcg gtgcttcgtt cgaccacgaa gagagacagg tcaccgcgtt gtcctggcgc
    10621 gtctccggta cgggctgaca ccagcagcag atcggcggcg tcaccccagg gcacggcact
    10681 cttgcggccg ctcaaccgaa atccctgctc caccttgtcg gcccgcgtgc tcacgcagtc
    10741 cagctcgtat cgggctccgg gctcgacgtg ggcgaccagg cccaaggcgg cgccttcgca
    10801 caggcggggc agccatgtcg cctgctgatc gggccgtccc agcagttgca acagatgtgt
    10861 ggcggccagg gcttcggcgt aaggcgcggg aacgaactgg gcgcccatcg cctcgatcag
    10921 cgacagcgcg gcgcggggcg actgggccat gccgccccat ggctcgccca gcgtgatccc
    10981 gagccaaccc agctcagcca tttgtcgcca caaggcgaga ttggacccgg cgtcggccga
    11041 ttgttgccgc tgcctatgcc gttcaaagcc gcactgttcg atcagaaatc ggtttgcgct
    11101 ctcgcgcagc gcgtcgtgct cttggtggaa agacatcgat tgcatccgct cgtcagatgc
    11161 caagcgcaaa cttggcaatg atgttgtgct gaacctcgtt ggagccgccg tagatcgaca
    11221 atttgcgcca gttcaggtat tggcgcacca gcgccatcgg agtgggcagg ccatcgccca
    11281 tcggcgccgt gcaatcgcca cacgcctgca ccagcagcag cgacagttgc tgggcgatct
    11341 cggtgccgcg aattttcagc gttgacgcct cggcgccgac gctccgaccc gcctgtactt
    11401 cggcgatcag gcgcagggtg gtctgctcaa gcgccagcag atcgacttcg acggcggcaa
    11461 tgcgctcggc aaagcggaca tcctgcagca gcgagcgccc cagcgcatcg ggctgcgccg
    11521 ccaggcgctt gagcaggcgc agctcgcgca gcgagcggcc gatcttgcca acattgcagc
    11581 gctcatgccc gagcagcgcc ttggccaccg tccagccctg gttcaaggga ccaaccaccc
    11641 ggctcagggg cacgcggacc tggtcgagga acacttcatt gacctcgtga cccccatcga
    11701 gcaggatcaa cggtcgaacc gtgatgcccg gcatgtccat gtccagcaac acgaaggaaa
    11761 tgccttcctg ggacttggcc tgggggtcgg tgcgcaccag gcagaacatg cggttggcgt
    11821 attgcgcgta ggtggtccag gtcttctgac catcgatcac cagggtgtcg ccttcgatgc
    11881 gaccacgcgt gcgcaggctg gccaggtccg atccggcgcc cggctccgaa tacccctgac
    11941 accaccagtc ctcgctgctc agaatgcgtg gcaaaaactc gtcctgctgc gccggcgtgc
    12001 cgtgctgcat caacaccggc gccagcatct tcggcccgaa ggcgaacacc tcgggcgcgc
    12061 ctgccagcca gcactcctct tcaaaaatgt agatctggcg cggcgtccat cccgtgccac
    12121 cgcgcgccac cggccaggct ggcgccagcc agccgcgcgc attcagaatg cgctgccagc
    12181 tgacgtagtc gtccttggtc aggggggcat ggcccagcac tttggctcgc agggtgtcgg
    12241 gcagggcctc gcgcagaaag gcgcgcactt cttcgcgcag ggcgcggtcc gcgtccagat
    12301 cgtgggcatg catggcgcgt cagtcgatgg tgatgttgtg ctggcggatc agcgcgcccc
    12361 agcgctcgcg gtcggccttc aggtattcgt cgacctgggc gggggtcatg gccggcagca
    12421 tcagcacacc ggccttggtg aaggtggcgc ggaatttttc gtcctgtgcg aagcggccaa
    12481 tggcgccctg caggcgggcc accacgtcgg gcggggtggc agccggcacg gcccagccca
    12541 accagctggc ggcgtggaag gcgccatagc ctgcttcttc cagcgtcggc acgtcaggca
    12601 gccacgaaat gcgcttgctc atcggtaccg ccagggcctt gagcttgccg ctctgaatga
    12661 acggcagcgc ccctggcagc acgtcgatgt agaactgcac atcgttggcc atcagcgccg
    12721 tcagcgacgg tgcgctgccc ttgtagggca cttcggtcat gcggatgccc aactcgccct
    12781 cgatcttgcg ggccgtcaga tacagcacat taccctgccc gagagaggca aagttcagtt
    12841 tgccgtcgtt ggcttttgca taggcggcaa acgagcgcag gtcggtggct ggcgtctgca
    12901 ggttggtcac cgccacggtc ggcaactcgc ccacgatggc gatcacgcgc agttccttga
    12961 tcgggtcata ggtgagtttt ttcatca
//
LOCUS       DAOQTZ010000081        12399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5776_length_12399_cov_13.475778, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000081 DAOQTZ010000000
VERSION     DAOQTZ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12399
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5776_length_12399_cov_13.475778"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKN51_09930"
     CDS             complement(<1..321)
                     /locus_tag="PKN51_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNT85459.1"
                     /translation="MRIGPYTLPNALFVAPMAGVTDRPFRQLCRAFGAGYAVSEMVTS
                     RRDLWHTLKTSRRADHVGEPGPIAVQIAGTDAPMMAEAARYNIERGAQIIDINMGCPA
                     KKVCN"
     gene            452..922
                     /locus_tag="PKN51_09935"
     CDS             452..922
                     /locus_tag="PKN51_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNT85460.1"
                     /translation="MEAWLQSLLAALALPEFGLSSVFVVAFISATLLPVGSEPAVFGL
                     VQLNPALLWPAIGVATVGNTLGGVVSWWMGLGAHHVADRYRHSPTHLRALDWLQRIGP
                     RACFLSFLPIIGDPLCAVAGWLRLPLLPCTVWMAAGKLLRYTGMTLGLLYLWPK"
     gene            1111..>1907
                     /locus_tag="PKN51_09940"
     CDS             1111..>1907
                     /locus_tag="PKN51_09940"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNT85461.1"
                     /translation="MSHAELIIRACRQIEAAEAPPSLAALAQAAGLSPHHFQRLFKAH
                     TGVTPRAYAEGQRARRLRARLTQAASVTEAIYDAGFNAPSRFYENARELIGMTPSRWR
                     AGGAGETIRFALGQCSLGAILVAESDQGICSIALGDAPEPLLAQLQAQFPRADLVGGD
                     AAFERRVAQVVGFVEQPALGLELPLDVRGSAFQQRVWQALRAVPAGQTLSYAELARRI
                     GQPRAARAVARACASNVLAVAIPCHRVVRGDGALAGYRWGVARKQALL"
     assembly_gap    1908..2007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2008..2879)
                     /locus_tag="PKN51_09945"
     CDS             complement(<2008..2879)
                     /locus_tag="PKN51_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT85462.1"
                     /translation="MDWDNLRFFLELARTGTLTAAARRLAVDHTTVARRLQALEKQAG
                     ATLFTREADGHRLTEAGQRLLPGAQAMERAAQQLALGAPAGGDGPAGVVRLGATEGFG
                     TALLAPRLAWLTRQHPGLTLDLLALPRLLHLSRREADIVISLERPRHETVVVTRLSDY
                     ALHLYGQREYLARRPLIQRREDLRHHAFVSYVDDLLFTKELQFLGELHQPARFALRST
                     SISAQLEAVRAGAGLAVLPAFLADQDPTLARVLPAEGRFIRTFWMSMPGEIRHQPAIQ
                     TVWGFLRQAVAEMQP"
     gene            2994..4511
                     /locus_tag="PKN51_09950"
     CDS             2994..4511
                     /locus_tag="PKN51_09950"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNT85463.1"
                     /translation="MDAATTPKVANVKLLINGQFIESRTTQWRDVVNPATQQVLARVP
                     FATPDEVNAAVAAAKQAFKTWRKTPIGTRARIFLKLQQLIRDNMKELAALLTAEQGKT
                     LPDAEGDVFRGLEVVEHAAAIGNLQLGELANNVASGVDTFTVLQPLGVCAGITPFNFP
                     AMIPLWMFPMAIATGNTFVLKPSEQDPMVTMRLCELALEAGIPPGVLNVVHGGEDVVN
                     AICDHQDIKAISFVGSTKVGTHVYNRASLAGKRVQCMMGAKNHAIVMPDANREQSINA
                     ILGASFGAAGQRCMAISVVLLVGEAQKWLPEFVEKAKTLKVSAGTTPGADLGPLISCA
                     ARSRVEGLIERGVAEGAKLELDGRNPSIDGFEKGNFVAPTIFSGVKPGMSIYDQEIFG
                     PVLCVVAADDLEQAIDFINANPNGNGTAIFTQSGAAARMFKEDIDVGQVGINLPVPVP
                     VPLFSFTGSRASKLGDLGPYGKQVVLFYTQTKTVTERWFDDSTLHHGVNTTISLR"
     gene            4721..5884
                     /locus_tag="PKN51_09955"
     CDS             4721..5884
                     /locus_tag="PKN51_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT85464.1"
                     /translation="MDFELSEEQRAFADTARQFAEAELAPHAARWDAEAHFPREAIAK
                     AGELGFCGLYAPESAGGLALPRLDATLVFEQMAAVDPSTTAFITIHNMATWMLGTWAT
                     PALREHWGPLLTSGQKLASYCLTEPGAGSDAASLKTRAERVGGEYVIHGGKAFISGAG
                     STDVLVLMARTGGGGAGGISAFVVPADLPGIHYGKKEEKMGWNSQPTRQIAFDGVRVP
                     ADHLLGQEGEGFRIAMKGLDGGRINIATCSVGAAQGALQHAQRYLHERQQFGQPLASF
                     QALQFKLADMATELVAARQMVRLAASKLDRGAPDASTYCAMAKRFATDAGFGVINDAL
                     QLHGGYGYIREYPLERLLRDARVHQILEGTNEIMRVIIARRLLQDSPMDGLTG"
     gene            5902..7038
                     /locus_tag="PKN51_09960"
     CDS             5902..7038
                     /locus_tag="PKN51_09960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT85465.1"
                     /translation="MSTTQAPVLFDTLATASGHRFGRATLNTPASLNALSLAMVDLLD
                     PQLAAWAADPQIAGVLLDAAGDKAFCAGGDVVALAREILALHSQQPGAVPQAAADFFE
                     REYRLDHRIHRYPKPLLVWGHGIVMGGGIGLMAGASHRVATPKTRLAMPEIGIGLYPD
                     VGGSWLLGRMPGRTGAFLALTGASINAADALFAGMADFVLPHEQHGALLQAIGQARWQ
                     GDQAADGAQLSHLLQRLGEGTALPASPLRTHLDRINAVIGHDRLSDIAPRLAALAQDA
                     DPWLAQAGASFVKGSPTSAALGLELQRRCRHLSLADTFRLEWQASVGCCAHQDFAEGV
                     RALLIDKDKAPRWQPATLEQVTPQWIEDHLRPRFAGAHPLADLA"
     gene            7091..7999
                     /gene="mmsB"
                     /locus_tag="PKN51_09965"
     CDS             7091..7999
                     /gene="mmsB"
                     /locus_tag="PKN51_09965"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HNT85466.1"
                     /translation="MSTPTRIAFIGLGHMGGPMATNLHKAGFAVSAFDLSADALAAVR
                     AQGVPTADSARAAVAGAQVVISMLPASRHVEGLYLGTGDAEGLLAAIEPGALLIDSST
                     IAPASAQKVAQAAAARGLAMIDAPVSGGVAGASAGTLTFIVGGDAAALERARPVLQAM
                     GKNIFHMGAIGAGQVAKLCNNMALGVIMAVTGEAIALGVAHGLDAKTLSDMMAVSTSR
                     SWATEVCNPWPGVHPNAPASRGYSGGFGNDLMLKDLGLAVEAAMGVGASIPLGELARN
                     LYALNKQAGRGGQDFSGVVQLLTRLP"
     gene            8228..9277
                     /locus_tag="PKN51_09970"
     CDS             8228..9277
                     /locus_tag="PKN51_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNT85467.1"
                     /translation="MTATATPTASNEVSRAAPELVCPAGSLPALKAAIDGGADCVYLG
                     LRDATNARNFAGLNFDEAAIQTGIAYAHARGRKVLLALNTYPQASNPAPWRSAMDRAA
                     AWGVDAIILADPGLMRYACERHPGLRLHLSVQGSATNHDAINLYHQQFNIQRAVLPRV
                     LSLEQVKQVVQRTPVEIEVFGFGSLCVMVEGRCALSSYATGESPNTHGVCSPAKFVRW
                     KETPSGVESRLNGVLIDKYGQGENAGYPTLCKGRFDVGEESNYYALEEPTSLNTLELL
                     PQLVKMGVRAFKIEGRQRSPAYVAQVTKVWREAIDHCMAQGHLYAPKTAWMASLDQVA
                     EGQQHTLGAYHRPWK"
     gene            9295..10194
                     /locus_tag="PKN51_09975"
     CDS             9295..10194
                     /locus_tag="PKN51_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNT85468.1"
                     /translation="MKLALGPLLYYWPRETVFEFYQAMAETAVDIVYVGEAVCSRRHE
                     LRHGDWLDIARMLRDAGKQAVLSTMVLLESTADVAAMHKIVREEGWLIEAGDMGAVHN
                     LAGQRPFVAGPQLNVFNADTLAWMASLGASRWVVPLEMRRDDLAVLQRERPAGLETEV
                     FAYGRMPLAFSARCFTARHYNLPKDDCGFACIQHPDGQLLKTREGEAFLVLNGIQTQS
                     ARVYNLIGDLPELRALGVDVLRLSPQSQHMADIVAAFDAARRADTPDPDALARLRPMM
                     PDEPCNGYWHGRSGMDLIEPALA"
     gene            10223..10744
                     /locus_tag="PKN51_09980"
     CDS             10223..10744
                     /locus_tag="PKN51_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNT85469.1"
                     /translation="MTTPPLPTPFTLPRPVGALLSCLPAYPGSVLLVSAINLALARQL
                     PEDVTALLKDKRLSIRVRDARLGFDFTWNGRRFAPSAPHAEPDLAISANAQDFLLLAQ
                     RRQDPDTLFFNRRLVMEGDTELGLVVKNALDALELPVLDPAAWSPRAVLARWAPGLAE
                     RLPPFPPFSAPRR"
     gene            10741..11136
                     /locus_tag="PKN51_09985"
     CDS             10741..11136
                     /locus_tag="PKN51_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNT85470.1"
                     /translation="MNPSGTHDHAPLVYSCSGCSSAAQLANHVALQLDRRGVAEMSCI
                     AGVGGDVPKLVRLATSGRPIVALDGCPLACVRSSLARHGVAPARHYQLHQYGVKKRTH
                     QDFDPAQAAEVLARIEADLRQHPLAPAHD"
     gene            11129..11713
                     /locus_tag="PKN51_09990"
     CDS             11129..11713
                     /locus_tag="PKN51_09990"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNT85471.1"
                     /translation="MTELRRVIVGITGASGAIYGVRLLQVLRELASVTTHLVVSDAGW
                     RSLRYELDLAPAQVQALADEAHDIRNIGACIASGSFVTLGMVVAPCSMRTLAAIAHGL
                     GDNLLTRAADVVLKERRRLVLLARESPLHLGHLRNMVAVTELGAIVSPPVPAFYKRPR
                     SLEDIVDHSVARALDALGIEHKLSTRWPGLPTDY"
     gene            11924..>12399
                     /locus_tag="PKN51_09995"
     CDS             11924..>12399
                     /locus_tag="PKN51_09995"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNT85472.1"
                     /translation="MPYRDLRDFMAQLEAAGELRRIAEPVSPHLEMTALADRVLRAGG
                     PALLFENPTGHRMPVLANLFGTPGRVARALGVAGLAEIRAFGEVLAQLKEPEPPRGVK
                     DLWGRRDLLKTLWSMAPSVVRRPPCQQIVWEGAEVDLARLPIQHCWPGDVAPLVTWG"
BASE COUNT         1834 a         4531 c         4198 g         1736 t
ORIGIN      
        1 gttgcacacc ttcttggccg ggcagcccat gttgatgtcg atgatctggg cgccgcgctc
       61 gatgttgtag cgcgccgcct cggccatcat gggcgcgtcg gtgccggcga tctgcaccgc
      121 gatcgggccg ggctcgccga cgtggtcggc gcggcgcgaa gtcttgagcg tgtgccacag
      181 atcgcgccgc gaggtcacca tctcgctgac cgcatagccg gccccgaagg cgcggcacag
      241 ctggcgaaac ggccggtccg tcacccccgc catgggggcc acgaacaggg cgttgggaag
      301 ggtgtaggga ccgattcgca tgggcacggg gcgcagcggc gtcggcctcg atgagccggg
      361 ggtgcgcgac agggaagcgg attctaactg cccaaaattt cagcaatcga aacgccgcgc
      421 cgccagccgc gatccctaca ctcgcgcctt gatggaagcc tggttgcaat cgctgctggc
      481 cgcgctggcg ctgcccgagt tcgggctgtc cagcgtgttc gtggtcgcct tcatctcggc
      541 gaccttgttg ccggtcggct ccgagccggc cgtgttcggc ctggtacagc tcaaccccgc
      601 actgctgtgg ccggccatcg gggtggccac cgtcggcaac acgctgggcg gcgtggtgag
      661 ctggtggatg ggactgggcg cccatcacgt ggccgaccgc tatcgccatt cgcccaccca
      721 cctgcgggcg ctggactggt tgcagcgcat cggcccgcgc gcctgctttc tgtcgtttct
      781 gcccatcatc ggcgacccgc tgtgcgccgt ggccggctgg ttgcgtctgc cgctgttgcc
      841 ctgcaccgtc tggatggcgg ctggcaagct gctgcgctac accggcatga ccctggggct
      901 gctctacctg tggccgaagt aacctggata tgctatcaat taaatagctt ctggcgcttg
      961 ccaggcaggc gccgacgccg tattcgatcc tagagaaggc agtggtccgc gtgtcatctt
     1021 cgggcaggcc gcatccctgc aggacatgag cggggacatg gccgcaagaa acggatggcc
     1081 ggccccggag gcgcgccgta cagtggcgcc atgagccatg ccgagctgat catccgcgcc
     1141 tgccgccaga tcgaggcggc cgaggcgccg ccttcgctgg ccgcgctggc gcaggcggcg
     1201 gggctgtcgc cgcaccactt ccagcgcctg ttcaaggccc acaccggggt gacaccgcgc
     1261 gcctatgccg agggccagcg cgcacgccgg ctgcgcgccc ggctgaccca ggcggccagc
     1321 gtgaccgagg ccatctacga cgccggcttc aatgcgccca gccgcttcta cgaaaacgca
     1381 cgcgagctga tcggcatgac gcccagccgc tggcgcgccg gcggcgcggg cgagacgatc
     1441 cgcttcgcgc tcggccagtg ttcgctgggc gcaatcctgg tggccgaaag cgaccagggc
     1501 atctgcagca ttgccctggg cgacgcaccc gagccgctgc tggcgcagct gcaagcgcag
     1561 tttccgcgcg ccgacctggt gggtggcgac gccgccttcg agcgccgcgt ggcgcaggtg
     1621 gtgggtttcg tcgagcagcc ggccctgggg ctggagctgc cgctggacgt gcgcggcagc
     1681 gccttccagc agcgcgtgtg gcaggcgctg cgcgcggtgc cggccggcca gacgctgagc
     1741 tacgccgagc tggcgcgccg catcggccag ccgcgcgccg cccgcgccgt ggcgcgcgcc
     1801 tgcgccagca acgtgctggc ggtggccatc ccttgccacc gcgtggtgcg cggcgacggc
     1861 gcgctggccg gctatcgctg gggcgtggcg cgcaagcagg cgctgctnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnggc tgcatttcgg ccaccgcctg gcgcagaaag
     2041 ccccacacgg tctggatcgc cggctggtgg cggatttcgc ccggcatgct catccagaag
     2101 gtgcggatga agcgcccttc ggccggcagc acgcgcgcca gcgtcgggtc ctggtcggcc
     2161 agaaaggccg gcagcacggc caggccggcg ccggcgcgca cggcttccaa ctgcgcgctg
     2221 atgctggtgc tgcgcagcgc aaaacgcgcc ggctggtgca gctcgcccag aaactgcaac
     2281 tccttggtga acagcaggtc gtcgacgtag ctgacgaagg cgtggtggcg caggtcctcg
     2341 cgccgctgga tcagcggccg gcgcgccagg tactcgcgct ggccgtacag gtgcagggcg
     2401 taatcgctca ggcgggtgac caccaccgtc tcgtggcgcg ggcgctccag cgagatgacg
     2461 atgtcggcct cgcgccgcga caggtgcagc agccgcggca gcgccagcag gtccagcgtc
     2521 agccccgggt gctggcgcgt cagccaggcc agccgcggcg ccagcagggc ggtgccgaag
     2581 ccctcggtgg cgcccagccg caccacgccg gccgggccgt cgccgccggc cggtgcgccc
     2641 agcgccagtt gctgggcggc gcgctccatg gcttgggcgc cgggcagcag gcgctggccg
     2701 gcctcggtca ggcggtggcc gtcggcctcg cgcgtgaaca gggtggcgcc ggcctgcttt
     2761 tccagcgcct gcaggcgccg cgccaccgtg gtgtggtcca ccgccaggcg gcgcgcggcg
     2821 gccgtcagcg tgccggtgcg cgccagttcc agaaagaagc gcaggttgtc ccagtccatg
     2881 ccgacctcgc cgcgttgtgc atttttgcag atcaagagtg cgtgcctgcg cattgtcatg
     2941 gcaaattcgc acagctacca tgcgtccatt ccatcccacc ccaggagaca cccatggacg
     3001 ccgccaccac ccccaaagtc gccaacgtca agctgctgat caacggtcag ttcatcgaat
     3061 cccgcaccac ccagtggcgc gacgtggtca acccggccac ccagcaggtg ctggcgcgcg
     3121 tgccgtttgc cacgcccgat gaggtcaacg ccgccgtcgc cgccgccaag caggccttca
     3181 agacctggcg caagaccccc atcggcacgc gtgcgcgcat cttcctgaaa ttgcagcagc
     3241 tgatccgcga caacatgaag gagctggccg cgctgctgac ggccgaacag ggcaagaccc
     3301 tgcccgacgc cgagggcgac gtgttccgcg gcctggaggt ggtggagcac gccgccgcca
     3361 tcggcaacct gcagctgggc gagctggcca acaacgtcgc cagcggggtg gacaccttca
     3421 ccgtgctgca gccgctgggc gtgtgcgccg gcatcacgcc gttcaacttc ccggccatga
     3481 ttccgctgtg gatgttcccc atggccattg ccaccggcaa caccttcgtc ttgaagccca
     3541 gcgagcagga ccccatggtc accatgcgcc tgtgcgagct ggcgctggaa gccggcattc
     3601 cgcccggcgt gctgaacgtg gtgcatggcg gcgaggacgt ggtcaacgcc atttgcgacc
     3661 accaggacat caaggccatc agcttcgtcg gttcgaccaa ggtgggcacc catgtctaca
     3721 accgcgccag cctggccggc aagcgcgtgc aatgcatgat gggcgccaag aaccacgcca
     3781 tcgtcatgcc cgacgcgaac agggagcagt cgatcaacgc cattctgggt gcgtcgttcg
     3841 gcgccgccgg ccagcgctgc atggcgatct cggtagtgct gctggtgggt gaagcgcaga
     3901 aatggctgcc cgagttcgtc gagaaggcca agacgctcaa ggtctcggcc ggcaccacgc
     3961 ccggtgccga cctgggcccg ctgatctcct gcgccgcgcg ctcgcgcgtc gaaggcctga
     4021 tcgagcgcgg cgtggccgaa ggcgccaagc tggaactcga cggccgcaac cccagcatcg
     4081 acggcttcga gaagggcaac ttcgtcgccc cgaccatctt cagcggcgtc aagcccggca
     4141 tgagcatcta cgaccaggag atcttcggcc ccgtgctgtg cgtggtcgcc gccgacgacc
     4201 tggagcaggc catcgacttc atcaacgcca acccgaacgg caacggcacc gccatcttca
     4261 cgcagtcggg cgcggccgcg cgcatgttca aggaagacat cgacgtcggc caggtcggca
     4321 tcaacctgcc ggtgccggtg cccgtgccgc tgttctcgtt caccggcagc cgcgcttcca
     4381 agctgggcga cctgggcccc tacggcaagc aggtggtgct gttctacacc cagaccaaga
     4441 ccgtcaccga gcgctggttc gacgacagca cgcttcacca tggcgtgaac accaccatca
     4501 gcctgcgctg agccacgcgc ccggcaggag gccggccgcc tcctgcccac ctctttttgg
     4561 gaagggcgcg gcggcgcggc ccatccgcgc ggccgggcat gggccgccgg ccgcgcgcaa
     4621 gccttctccc caagggcctg gaaaaaagca tcaaaacagg ctgtagcgca cgaccagcaa
     4681 gcgccagcag ctattaaaac gagagcagga gaagccgccc atggactttg aactgagcga
     4741 ggagcaacgg gccttcgccg acaccgcgcg gcagtttgcc gaggccgagc tggccccgca
     4801 cgccgcgcgc tgggacgccg aggcgcactt tccgcgcgag gccatcgcca aggcgggcga
     4861 gctgggcttc tgcggcctgt acgcacccga gtccgccggc ggcctggcgc tgccgcggct
     4921 cgacgccacc ctggtgttcg agcagatggc ggcggtcgac ccctccacca ccgccttcat
     4981 caccatccac aacatggcca cctggatgct gggcacctgg gccacgccgg cgctgcgcga
     5041 gcactggggc ccgctgctga ccagcggcca gaagctggcc agctactgcc tgaccgagcc
     5101 gggcgccggc tccgacgccg cctcgctcaa gacgcgcgcc gagcgggtgg gcggcgagta
     5161 cgtgatccac ggcggcaagg ccttcatcag cggcgccggc agcaccgacg tgctggtgct
     5221 gatggcgcgc accggcggcg gcggcgccgg tggcatcagc gccttcgtgg tgccggccga
     5281 tctgcccggc atccactacg gcaagaagga agaaaaaatg ggctggaaca gccagcccac
     5341 gcgccagatc gccttcgacg gcgtgcgcgt gccggccgac cacctgctgg gccaggaggg
     5401 cgaaggcttt cgcatcgcca tgaaggggct ggacggcggg cgcatcaaca tcgccacctg
     5461 ctcggtcggc gccgcccagg gcgcgctgca gcacgcccag cgctacctgc acgagcgcca
     5521 gcagttcggc cagccgctgg ccagcttcca ggcgctgcag ttcaagctgg ccgacatggc
     5581 caccgagctg gtggcggcgc gccagatggt gcgcctggcc gccagcaagc tggaccgcgg
     5641 cgcgcccgat gccagcacct actgcgccat ggccaagcgc tttgccaccg acgccggctt
     5701 tggcgtcatc aacgacgccc tgcagctgca cggcggctac ggctacatcc gcgagtaccc
     5761 gctcgagcgc ctgctgcgcg acgcccgcgt gcaccagatc ctggaaggca ccaacgagat
     5821 catgcgcgtc atcatcgcgc gccgcctgct gcaggacagc cccatggacg ggctgaccgg
     5881 ctgaccccca cgaccgacgc catgagcacc acccaagccc ccgtcctgtt cgacaccctc
     5941 gccaccgcca gcggccaccg cttcggccgc gccacgctca acaccccggc cagcctgaac
     6001 gcgctgtcgc tggccatggt cgatctgctc gacccgcaac tggccgcctg ggccgccgac
     6061 ccgcagatcg ccggcgtgct gctcgacgcc gccggcgaca aggccttctg cgccggcggc
     6121 gacgtggtgg cgctggcgcg cgagatcctt gccctgcact cgcagcagcc cggcgccgtg
     6181 ccacaggcgg cggccgactt cttcgagcgc gaataccggc tcgaccaccg catccaccgc
     6241 taccccaagc cgctgctggt gtgggggcac ggcatcgtca tgggcggcgg catcggactg
     6301 atggccggcg cctcgcaccg cgtggccacg cccaagacac gcctggccat gcccgagatc
     6361 ggcatcggcc tgtaccccga cgtcggcggc tcctggctgc tggggcgcat gccgggccgc
     6421 accggtgcct tcctggcgct gaccggcgcc tcgatcaacg ccgccgacgc gctgtttgcc
     6481 ggcatggccg acttcgtgct gccgcacgag cagcacggcg cgctgctgca ggccatcggc
     6541 caggcgcgct ggcagggcga tcaggcggcc gacggcgcgc agctcagcca cctgctgcag
     6601 cggctgggtg aaggcacggc gctgcccgca tcgcccctgc gcacccacct ggaccgcatc
     6661 aacgccgtca tcggccacga ccgcctgagc gacatcgccc cgcgcctggc ggcgctggcg
     6721 caggacgccg acccctggct ggcgcaggcc ggcgccagct tcgtcaaggg ctcgcccacc
     6781 tcggccgccc tggggctgga attgcagcgg cgctgccgcc acctgtcgct ggccgacacc
     6841 ttccggctcg aatggcaggc ctcggtgggc tgctgcgcgc accaggactt cgccgaaggc
     6901 gtgcgcgccc tgctgatcga caaggacaag gcgccgcgct ggcagccggc cacgctggag
     6961 caggtcacgc cgcagtggat cgaagaccac ctgcggccgc gctttgccgg cgcgcacccg
     7021 ctggccgacc tggcttgagc gagcccccgc acccgtacac accccatcca ttgcttcaag
     7081 gagacaacac atgagcaccc ccacccgcat cgccttcatc ggcctgggcc acatgggcgg
     7141 cccgatggcc accaacctgc acaaggccgg ctttgccgtc agcgccttcg acctcagcgc
     7201 cgacgccctg gccgccgtgc gcgcccaggg cgtgccgacc gccgactccg cccgcgcggc
     7261 cgtggccggc gcgcaggtgg tgatctccat gctgccggcc agccgccacg tcgaaggcct
     7321 gtacctgggc acgggcgacg ccgagggcct gctggccgcc atcgagccag gcgcgctgct
     7381 gatcgactcc agcaccatcg cccccgcctc ggcccagaag gtggcccagg ccgccgccgc
     7441 gcgcggactg gccatgatcg acgcgccggt gtcgggcggc gtggccggcg ccagcgccgg
     7501 cacgctgacc ttcatcgtcg gcggcgacgc cgcggcgctg gagcgcgccc gcccggtgct
     7561 gcaggccatg ggcaagaaca tcttccacat gggcgccatc ggcgccggcc aggtggccaa
     7621 gctgtgcaac aacatggcgc tgggcgtgat catggccgtc actggcgaag ccatcgccct
     7681 gggcgtggcg cacgggctgg acgccaagac gctgtccgac atgatggccg tcagcaccag
     7741 ccgcagctgg gccaccgaag tctgcaaccc ctggcccggc gtgcacccca acgccccggc
     7801 ctcgcgcggc tacagcggcg gcttcggcaa cgacctgatg ctcaaggacc tgggcctggc
     7861 ggtcgaggcc gccatgggcg tgggcgccag cattccgctg ggcgagctgg cgcgcaacct
     7921 gtacgccctg aacaaacagg ccggccgcgg cgggcaggac ttctccggcg tcgtgcagct
     7981 gttgacccgg ctgccctgag cccaggctga acgacacgac gcgcccggac cggggcgggc
     8041 ccagccggtg gcgcaatccc gagtgatttc ttcgggaata tccggtttcg gttgaccgag
     8101 gtcataacgc ggcgccggcg atcggcctac agtgcccggt ctgggcgcgc tgcgtggcct
     8161 gactcgtccg gtcatgacgg cgcacgctgg cccagcgcat ggcgttcgtt cgccgaagga
     8221 agtgcccgtg accgccaccg cgacccccac cgcctccaac gaggtcagcc gcgccgcccc
     8281 cgaactggtc tgcccggccg gcagcctgcc ggcgctgaaa gccgccatcg acggcggcgc
     8341 cgactgcgtc tacctgggcc tgcgcgacgc caccaacgcg cgcaatttcg ccggcctgaa
     8401 cttcgacgag gcggccatcc agaccggcat cgcctatgcc cacgcgcgcg gccgcaaggt
     8461 gctgctggcg ctcaacacct atccgcaggc cagcaacccg gcgccctggc gctcggccat
     8521 ggaccgcgcc gccgcctggg gtgtggacgc catcatcctg gccgacccgg ggctgatgcg
     8581 ctacgcctgc gagcgccacc ccggcctgcg actgcacctg tcggtgcagg gctcggccac
     8641 caaccacgac gccatcaacc tgtaccacca gcaattcaac atccagcgcg cggtgctgcc
     8701 gcgcgtgctg tcgctggagc aggtcaagca ggtggtgcag cgcacgccgg tggagatcga
     8761 ggtgtttggc tttggcagcc tgtgcgtgat ggtcgagggg cgctgcgcgc tgtcgtccta
     8821 cgccaccggc gagtcgccca acacccatgg cgtgtgctcg ccggccaagt tcgtgcgctg
     8881 gaaggagacg cccagcggcg tggagtcgcg cctgaacggc gtgctgatcg acaagtacgg
     8941 ccagggcgaa aacgccggct acccgacgct gtgcaagggc cgcttcgacg tgggcgagga
     9001 gagcaactac tacgcgctgg aagagcccac cagcctcaac accctggagc tgctgcccca
     9061 gctcgtgaag atgggcgtgc gcgccttcaa gatcgagggc cgccagcgca gccccgccta
     9121 tgtggcgcag gtcaccaagg tctggcgcga agccatcgac cactgcatgg cgcagggcca
     9181 cctgtacgcg cccaagaccg cctggatggc cagcctggac caagtggccg agggccagca
     9241 gcacacgctg ggcgcctacc accggccatg gaaatgatcg acatgagcac gacgatgaaa
     9301 ctggccctgg gcccactgct gtactactgg ccacgcgaga cggtgttcga gttctaccag
     9361 gccatggccg agaccgcggt ggacatcgtc tacgtgggcg aggccgtgtg ttcgcgccgc
     9421 cacgagctgc gtcacggcga ctggctggac atcgcgcgca tgctgcgcga tgccggcaag
     9481 caggccgtgt tgtcgaccat ggtgctgctg gaatcgacgg ccgacgtcgc cgccatgcac
     9541 aagatcgtgc gcgaggaagg ctggctgatc gaggccggcg acatgggcgc cgtgcacaac
     9601 ctggccggcc agcggccctt cgtggccggg ccgcagctca acgtcttcaa cgccgacacg
     9661 ctggcctgga tggccagcct gggcgccagc cgctgggtcg tgccactgga gatgcgccgc
     9721 gacgatctgg ccgtgctgca gcgcgagcgg ccagccgggc tggaaaccga ggtgttcgcc
     9781 tatggccgca tgccgctggc gttttcggcg cgctgcttca ccgcgcgcca ctacaacctg
     9841 cccaaggacg actgcggttt tgcctgcatc cagcaccccg acgggcagtt gctgaagacg
     9901 cgcgagggcg aggccttcct ggtgctcaac ggcatccaga cgcagtcggc gcgcgtctac
     9961 aacctgatcg gcgacctgcc cgagctgcgc gccctgggcg tcgacgtgct gcgcctgtcg
    10021 ccgcagtcgc agcacatggc cgacatcgtc gccgccttcg acgccgcgcg ccgcgccgac
    10081 acgcccgacc ccgacgcgct ggcacgcctg cgcccgatga tgcccgacga accctgcaac
    10141 ggctactggc acgggcgctc cggcatggac ctgatcgagc cggccctggc ctgaacgccg
    10201 gcgcacgtgc gaggagcctg tcatgaccac gccccccctg cccaccccct tcaccctgcc
    10261 gcgtccggtg ggtgcgctgc tgtcgtgcct gccggcctac cccggctcgg tgctgctggt
    10321 cagcgccatc aacctggcgc tggcgcgcca gctgcccgag gacgtgacgg cgctgctgaa
    10381 ggacaagcgc ctgtcgatcc gcgtgcgcga cgcccgcctg ggtttcgact tcacctggaa
    10441 cggccggcgc tttgccccca gcgcgccgca cgccgagccc gacctggcca tcagcgccaa
    10501 cgcgcaggac tttctgctgc tggcccagcg ccgccaggac ccggacacgc tgttcttcaa
    10561 ccgccgcctg gtgatggagg gcgacaccga gctcggcctg gtggtcaaga acgcgctcga
    10621 cgcgctggag ctgccggtgc tcgacccggc ggcctggagc ccgcgcgccg tgctggcgcg
    10681 ctgggcgccg gggctggccg agcggctgcc gcccttcccg cccttttcgg cgccgcgccg
    10741 atgaacccgt ccggcacgca cgaccacgcg ccgctggtgt actcctgctc gggctgctcc
    10801 agcgccgcgc aactggccaa ccacgtggcg ctgcagctcg accgccgcgg cgtcgccgag
    10861 atgtcctgca tcgccggcgt gggcggcgac gtgcccaagc tggtcaggct ggccaccagc
    10921 ggccggccca tcgtggcgct cgacggctgc ccgctggcgt gcgttcgaag ctcgctggca
    10981 cgccacgggg tggcgccggc gcgccactat cagctgcacc agtacggcgt gaagaagcgc
    11041 acgcaccagg acttcgaccc ggcccaggcg gccgaggtgc tggcgcgcat cgaagccgac
    11101 ctgcggcagc atccgctggc gcccgcccat gactgagctg cgccgcgtca tcgtcggcat
    11161 caccggcgcc agcggcgcca tttatggcgt gcggctgctg caggtgctgc gcgagctggc
    11221 cagcgtcacc acgcacctgg tggtgtcgga cgccggctgg cgcagcctgc gctacgagct
    11281 ggacctggca ccggcgcagg tgcaggccct ggccgacgag gcgcacgaca tccgcaacat
    11341 cggcgcctgc attgccagcg gctccttcgt cacgctgggc atggtggtgg caccctgctc
    11401 catgcgcacg ctggcggcga ttgcccacgg cctgggcgac aacctgctga cgcgcgccgc
    11461 cgacgtggtg ctgaaggaac gccgccgcct ggtgctgctg gcgcgcgagt cgcccctgca
    11521 cctgggccac ctgcgcaaca tggtggcggt gaccgagctg ggtgccatcg tcagcccgcc
    11581 ggtaccggcg ttctacaagc gcccgcgcag cctggaagac atcgtcgatc acagcgtggc
    11641 acgcgcgctg gacgccctgg gcatcgaaca caagctcagc acccgctggc cggggctgcc
    11701 gacggactat tgaaatcata gctgccggcg cacgtccgac aagcgctgca gcattgtttt
    11761 gcacttatgt ccagctgcct cgcaggcccc tcaacccact ctctctccag agggacgggg
    11821 gcgatcgcgc gccgtggttt tcactgccac tccctccggg agaggactgg ggtgagggcc
    11881 ggcaggcgcc atcagggccg cacctgacaa gccgcccctg accatgccct accgcgacct
    11941 gcgtgacttc atggcccagc tggaggccgc aggcgagctg cgccgcattg ccgagccggt
    12001 ctcgccgcac ctggagatga cggcccttgc cgaccgcgtg ctgcgcgcgg gcggcccggc
    12061 cctgctgttt gaaaacccca cgggtcaccg catgccggtg ctggccaacc tgttcggcac
    12121 acccggccgc gtggcgcgcg cgctgggcgt ggcgggcctc gccgagatcc gcgcctttgg
    12181 tgaggtgctg gcccagctca aggagcccga gccgccacgc ggcgtcaagg acctctgggg
    12241 ccggcgcgac ctgctcaaga ccctgtggag catggcgccc tcggtcgtgc gccgcccgcc
    12301 ctgccagcag atcgtgtggg agggcgcgga ggtcgatctg gcgcgcctgc ccatacagca
    12361 ttgctggccc ggcgacgtgg cgccgctcgt cacctgggg
//
LOCUS       DAOQTZ010000082        12204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5890_length_12204_cov_14.598568, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000082 DAOQTZ010000000
VERSION     DAOQTZ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5890_length_12204_cov_14.598568"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="PKN51_10000"
     CDS             <1..1404
                     /locus_tag="PKN51_10000"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNT85473.1"
                     /translation="DGEFIQGGGRVEQPVLDPATGNTIGQLPHATRADLDRALAAAER
                     AFATWRHVSPMERSAILRKVGQLSRERAKDIGRNITLDQGKPLAEAVGEVLRCADHCD
                     WHAEECRRIYGRVIPPRTPNVRQFVLREPIGVCAAFTPWNFPYNQAIRKIAAAIGAGC
                     TIVIKGAEDAPSAVVAIARLFHEAGLPPGVLNVVWGVPHEVSEHLIASPIVRKVSFTG
                     SVPVGKQLAALAGSHMKRITMELGGHSPVIVCADADVERAATMLAASKFTNAGQVCVS
                     PTRFYIQEPVWDRFMASFTELSRQIKVGPGLEADTRMGPLAHARRVTAMAEFVEDAAA
                     RGARIETGGTRLGEVGNFFAPTVIAAPPDDARIMQAEPFGPIAACVPFKDLDEALQRA
                     NSLPFGLSSYAFTSNTRHALQIQNGLEAGMVNINHFGQALPETPFGGIKDSGMGSEGG
                     SETFDGYLVTKFVTQMD"
     gene            1485..3068
                     /locus_tag="PKN51_10005"
     CDS             1485..3068
                     /locus_tag="PKN51_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT85474.1"
                     /translation="MRQVLSTSVLAACLSATATWAATPADTLVIARDIGTILSLDPQE
                     AFEIAPGDALNSLYLRLVQHDPADFSKIVPGVAQSWQAAPDGKQIVFQIRKDLKFQSG
                     NPVTAEDAAFSLQRGILLDKQPAIILRQFGWTKDNVKQRVRADGDKLVLSFDQPYSTE
                     LILSALSAAIASVVDMKLVMSNERNGDLGNGWLRTRSAGAGAYKLVEWKAKDVVVLEA
                     FPGFKGGSPAKLKRVILRHIAEPATQRLLLEKGDVDVASDLKADQLQSIKGNANLKMI
                     QIPRGTVYYLALNTANPNLSKPEVWQAMRWLIDYDGIANKLFSGQYRVNQSAVARGTP
                     GALTEEPYKLDPAKAKALLAKAGFPNGFSMSLETSGASPYHEVSQSLQSTFAQGGIKV
                     ELRMGEPSQVMTRYRERRHDALVFVWAPDYSDPSSTLEFFSRNTDDSDKASNKNAPWR
                     AHWLTPQLTAEADKASHEVDDAKRMKIYADIQRKLRDESPFTFLIQKLEPIAMRTNVH
                     NYSGAVTFDSTPYWMIEKR"
     gene            3074..4159
                     /locus_tag="PKN51_10010"
     CDS             3074..4159
                     /locus_tag="PKN51_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009914885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT85475.1"
                     /translation="MATAAADDGVDIAPRRRLRSVRLRRGLRWLGATLLTSCITLFGL
                     LVVTFALGRVLPADPVLRIVGDRASQEQYDEVFRQLRLDRPLTEQFAAYVGDMVRGDF
                     GLSLVTRNPIGSDIARYFPATFELATVAIILGVVFGIPIGVVCAQYANRLPDHLGRML
                     SLIGYSVPVFWLGIVGLLVFYARLEWVAGPGRLDTAYRYSVPEVTHLMLIDTVLAGEW
                     DAFRNALSHLVLPASLLGLIALGYIARMTRSFLLWQLRQDYTTVLRVKGLSEAAIIWR
                     HALFNAAGPVLAIVALTYAYLLEGAVLTETVFAWPGLGMYITDSLFASDVPAVLTATL
                     LVGVIFMVVNIASEVIQAALDPRSRLA"
     gene            4156..5061
                     /locus_tag="PKN51_10015"
     CDS             4156..5061
                     /locus_tag="PKN51_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT85476.1"
                     /translation="MNPSSTLRQWLASDTPATRTQARLGAIYRLARRFLTHPIGVLGL
                     AMVASIVLAAGLAPWLPLADPIAQSLDQRLLPPSAAHWLGTDTLGRDILARVVHGARP
                     TLAIVALVLVISVPVGLLVGAAAGLWGGWIDAVLMRVADVFLAFPRLILAIAVAAALS
                     AGLWTAVIAIAATGWPPYARVARAEIAALRRSEFVQAARAIGVSPLRLLFRHALPLSL
                     PSAFVRAALDAPGIVLIVAGLGFLGVGLPPPQPEWGAMVAEGRAIVFEAWWVSTLPGI
                     MILWLSLGFNFVGDAMRDVVDPGVK"
     gene            5069..5926
                     /locus_tag="PKN51_10020"
     CDS             5069..5926
                     /locus_tag="PKN51_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85477.1"
                     /translation="MNHIPRDSAPPLLDIDNLRISFQTAHGRTEAVRGISLRMGRERV
                     AVVGESGSGKSITFRSLFGLLPATASVRADRMQLQGQDLLSLDRRDLRAIRGRRIGMV
                     VQDPKQGLDPLMTIGRQLIEMLRLHRKVPAAGAYQRAVELLAEVHIRDPKRVMGLYPH
                     EVSGGMAQRAMLAMILSAEPELLIADEATSALDAVVRHRILDLIEEQVRLRGMGLVLI
                     SHDLDLVARYADRILVMYAGRVMEELRGGELSAAQHPYTRGLLACRPSLDHPGQDLPV
                     LQREASWLQ"
     gene            5914..6663
                     /locus_tag="PKN51_10025"
     CDS             5914..6663
                     /locus_tag="PKN51_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85478.1"
                     /translation="MAAVNPPALRIDQLIVQRGSAQVVNGVSFSVPHGGSLGIVGESG
                     CGKSTILRAVAGIDTQWTGRIEIAGRPIGVQRSLADRRAMQMVFQDPLAALNPSHTID
                     EILREPLIVHRMPDQDARIVQALQTVALPLAVRFRFPGQLSGGQRQRVCIARALLVEP
                     QVLLLDEPTSALDVSVQAEVLNLLSRLRRERQQAFVLVSHDLHVVAHMCDQIAFMVGG
                     QFTDVLSRDRLRGATLHHPEAQRLLDAALTH"
     gene            6764..7963
                     /locus_tag="PKN51_10030"
     CDS             6764..7963
                     /locus_tag="PKN51_10030"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNT85479.1"
                     /translation="MTRSAFDPTAASGSAAPLLPLAGVRVVEFCTTASGPFSTMLLSD
                     MGADVVKIEPPTGDGLRQWPPLNQGYSENFASLNRNKRSAVLNLKDPQDVALARRLIL
                     EADVLVENNRPGVMDRLGLGYAALSAEKPELLFCSISAYGQSGPRASEGGFDLTIQAA
                     AGVMSVTGEPAGAPVKCGVPLSDFASGLYAAFSIASMLAHVRAGGRGAHIDIPMFGCT
                     LGIAALQTSEYFGNGRSPGKLGSAHPRNAPYQAFQAQDGYFAIAAGNHKLWLDVLRVV
                     QMPALAEDPRFLSTTDRARNQQALKEILEEVFTQRPVAHWIDAFNQAGVPHARINDYA
                     AALADPQTVHMGWVQPLTLPNGQQTRTFASPVRLNGQGFPIRRSPPELGQHTQEIRAE
                     CARSEVN"
     gene            7963..8556
                     /locus_tag="PKN51_10035"
     CDS             7963..8556
                     /locus_tag="PKN51_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HNT85480.1"
                     /translation="MAAESPLLTFVAAMTRLVESGPADEARLLDGARPLMKALVAQDD
                     WLPDAMAAAHPQYYQQHLLYGDPLDRFSLVSFVWGPGQKTPVHDHTVWGIIGMLRGAE
                     CAQRYTRDHQGRVTACGAEETLRPGDIDVVSPTLGDIHVVRNGLEDQVSISIHLYGGN
                     IGRIARHVFVPETDTVKDFVSGYSNTLSPNLWARQAA"
     gene            8553..9311
                     /locus_tag="PKN51_10040"
     CDS             8553..9311
                     /locus_tag="PKN51_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT85481.1"
                     /translation="MSQPELLSRRDGPVLHLQLHRPEVGNALSAALVQALTEAVDAAT
                     HDVSVRLLVLSGAGRHFCTGFDLSDLDRETDDSLLARFTRVELLLQAVHAAPFATLAL
                     AHGRTMGAGADLFAACGDRWIVGDAGFAFPGAAFGLVLGTVRLTELVGAPHAAAWIEG
                     GQRIDADQALACGLATRHIETEQIDAELGRWGARVNRLDAPTQRAIQAAIAAGRRPRG
                     DAGDAQDLARLVRSAARPGLRDRIAAYRAAQRKG"
     gene            9345..11471
                     /locus_tag="PKN51_10045"
     CDS             9345..11471
                     /locus_tag="PKN51_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNT85482.1"
                     /translation="MTSPTSSHSLKSLLNPRSVAVIGASEDQTKFGGRLYKTLLQHHY
                     DGAVYPINPTRDQLFGLKTYPSVTDTPQAPDMVVMALPRDKVKDEIAACAARGAKAGI
                     IITSKFSDAGPEGLALEREVVATAAAHGMRLIGPNCLGLISPANKLVLCSSPAVNVPQ
                     LIEAPIGFISQSGALMGTLFDRSYGMGIGFSHCVSVGNQADLELNDFVEFLIEDERTQ
                     VICSYVEGIKTPQRFVELARRARAAGKPWLMVKAGATDDGRRAAYSHTASLAGDFAAL
                     KAICERENVVLMDDPLDMLSLARAMVRYPRRQVRNVAVFTTSGGGGAITADQLSRAGV
                     GLSTFSDATMAALSEHYSEGQGANPIDVGGRKPDATSDIGAVTAELALADPGVDLGLM
                     VLTTAPDVPGLTRQMADGADRPAAGGKPTLYVMLPGRVAAPAREFLVQRSLPYVDTLA
                     EAMAVLRGWKAWSTYQEPAAPVRPADMAATPVPAAGTLGEAASKTLLGQAGIPVNIER
                     LVRSADEAAEAAAELGFPLVLKVVSPDIAHKSDVGGVALNILDPDDLRRRLALMQASV
                     ARAAPSARIDGFSLQRQEAGELELIVGARLDPQFGPQVLVGAGGVLVELLKDIAVLPA
                     PVDAASARRAVENLKIAPLLRAYRGRAALDVDAAVDVIVRLGWLAHDLAGSSGRDFEI
                     EVNPLKLRQQGHGAVAVDARARIAQA"
     gene            11511..>12204
                     /locus_tag="PKN51_10050"
     CDS             11511..>12204
                     /locus_tag="PKN51_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNT85483.1"
                     /translation="MAQITIEHHGKVTLFTINRPARKNAICKDTALELQAGFAEFDAS
                     EQRVAVITGAGNDAFSSGADVSNLPELWRCIPGSGFTTDKPIIAATAGWVVGGAMVLT
                     MMCDLIVAAENTRFSYPEARLGFTGGMIAGLAARIPHKAAMDLMLLGRTMDAQRAYGI
                     GLVSDVVPTGKQLEIAMEQAQTLAASAPLVLTTIKRFVNQHVLPKSPAEAMSETLRQL
                     NVVRESADIKEGM"
BASE COUNT         1907 a         4331 c         3994 g         1972 t
ORIGIN      
        1 gacggcgaat tcatccaggg cggcggccgg gtcgagcaac ccgtgctgga ccccgccacc
       61 ggcaacacca tcggccagct gccgcatgcg acccgggccg atctggatcg cgcgctggcg
      121 gcggccgagc gcgccttcgc cacctggcgc catgtctcgc cgatggagcg cagcgccatc
      181 ctgcgcaagg tcggtcagct cagccgtgaa cgcgccaagg acatcggccg caacatcacg
      241 ctcgatcagg gcaaaccact ggccgaggct gtgggcgaag tgctgcgctg cgccgaccat
      301 tgcgactggc acgccgagga gtgccgccgc atctatggcc gcgtgattcc gccgcgcacg
      361 ccgaacgtgc ggcagttcgt gctgcgcgag ccgatcggcg tgtgcgccgc cttcacgccc
      421 tggaacttcc cctacaacca ggcgatccgc aagatcgccg cggccatcgg ggcgggctgc
      481 accatcgtca tcaagggtgc cgaggatgcc ccgagtgccg tggtggccat tgcgcggctg
      541 ttccacgaag ccgggctgcc ccccggcgtg ctgaacgtgg tgtggggcgt gccccatgag
      601 gtatcggagc acctgatcgc ctcaccgatc gtgcgcaagg tgtccttcac cggctcggtg
      661 ccggtgggca agcagctggc cgccctggcg ggcagccaca tgaagcgcat cacgatggag
      721 ctgggcggcc attcgccggt catcgtgtgc gccgatgccg acgtcgagcg ggcggccacc
      781 atgctggccg cgtccaagtt caccaacgcc gggcaggtct gcgtctcgcc cacgcgcttc
      841 tacatccagg agccggtctg ggatcgcttc atggccagct tcaccgaact cagccgccag
      901 atcaaggtcg ggccgggact ggaggcggac acgcgcatgg gaccgctggc ccacgcgcgc
      961 cgcgtgacgg cgatggccga gttcgtcgaa gacgctgccg cgcgcggcgc ccgcatcgaa
     1021 accggcggca cgcggctggg cgaggtcggc aacttcttcg ctccgaccgt gattgccgcg
     1081 ccacccgacg acgcccgcat catgcaggcc gagccgtttg gccccattgc cgcctgcgtc
     1141 ccgttcaagg acctggacga ggccctccag cgcgccaaca gcctgccgtt cggcctgtcg
     1201 tcctacgcct tcaccagcaa cacgcgccac gccctgcaga tccagaacgg cctggaggcc
     1261 ggcatggtca acatcaacca cttcggccag gcgctgcctg aaacgccgtt tggcggcatc
     1321 aaggacagcg gcatgggcag cgaaggcggc agcgaaacct ttgacggcta cctggtcacc
     1381 aagttcgtga cccagatgga ctgacccgct tgcctgcgac caacaccgac gcgatgtcac
     1441 accctgactc catccaccag aggaccttcc ccatgaaaaa atcgatgaga caagttctga
     1501 gcacctccgt gctggccgcc tgcctgagcg ctacggcgac ctgggcagcc acccccgccg
     1561 atacgctggt gatcgcccgc gacattggca ccatcctcag cctggatccc caggaggcgt
     1621 ttgaaatcgc cccgggcgac gcactcaaca gcctctacct gcgcctggtg cagcacgatc
     1681 ccgccgattt ttccaagatc gtcccgggcg tggcgcagag ctggcaggcc gcacccgacg
     1741 gcaagcagat cgtgttccag atccgcaagg atctgaagtt ccagtcgggc aacccggtga
     1801 cggccgaaga cgccgcgttc tcgctgcagc gcggcatcct gctggacaag caacccgcca
     1861 tcatcctgcg ccagttcggc tggaccaagg acaacgtcaa gcagcgggta cgcgccgatg
     1921 gcgacaagct ggtgctgagc tttgaccagc cctactcgac cgagctgatc ctcagcgcgc
     1981 tcagcgccgc catcgcttcg gtggtggaca tgaagctggt gatgtccaac gaacgcaacg
     2041 gcgatctggg caacggctgg ctgcgcaccc gatcggcagg cgccggcgcc tacaagctgg
     2101 tggaatggaa ggccaaggac gtggtggtgc tggaggcctt cccgggcttc aagggcggct
     2161 caccggccaa gctcaagcgc gtcatccttc gacacatcgc cgaacctgcg acgcagcgcc
     2221 tgctgctgga aaagggcgat gtggacgtgg cctccgacct gaaggcagac cagctgcagt
     2281 cgatcaaggg caacgccaac ctgaagatga ttcagattcc gcgtggcacc gtctactacc
     2341 tggccctcaa cacggccaac cccaacctgt ccaaacccga agtctggcag gcgatgcgct
     2401 ggctgatcga ctacgacggc attgccaaca aactgttttc cggccagtac cgggtcaacc
     2461 agtcggccgt ggccaggggc acccccggcg ccctgaccga agagccctac aagctcgatc
     2521 cggccaaggc caaggccctg ctggccaagg ccggctttcc aaacgggttc tcgatgtcgc
     2581 tcgaaacctc gggtgccagc ccctatcacg aagtgtcgca gtcgctgcag tcgaccttcg
     2641 cccagggcgg catcaaggtg gagctgcgca tgggcgagcc gtctcaggtg atgacgcgct
     2701 accgtgagcg tcgacacgac gcgctggtgt tcgtctgggc gcccgactat tccgacccca
     2761 gctcgaccct ggagttcttc agccgcaaca ccgatgactc ggacaaggcc agcaacaaaa
     2821 atgcgccctg gcgcgcccac tggctgacgc ctcagctgac cgccgaggcg gacaaggcct
     2881 cgcacgaggt cgatgacgcc aagcgcatga agatctacgc cgacatccag cgcaagctgc
     2941 gtgacgagtc gccgttcacc ttcctgatcc agaagctgga gccgattgcc atgcgcacca
     3001 acgtgcacaa ctactcgggc gccgtcacgt ttgactccac gccctactgg atgattgaga
     3061 agcggtgaac ccgatggcca ctgcagcagc cgacgacggc gtcgacatcg ccccacgccg
     3121 gcgcctgcgc tcggtgcggc tgcggcgtgg cctgcgctgg ctgggtgcca ccctgctgac
     3181 ctcatgcatc acgctgttcg gtctgctggt ggtcaccttc gccctggggc gcgtgctgcc
     3241 ggccgatccg gtgctgcgca tcgtcggcga tcgcgcctcg caggagcagt acgacgaggt
     3301 gttccgccaa ctgcgcctgg accggccact gaccgagcag ttcgccgcct atgtgggtga
     3361 catggtgcgc ggcgacttcg gactgtcgct ggtcacgcgc aaccccattg gttcggacat
     3421 cgcgcgctac tttcccgcca cgttcgagct ggcgacggtg gccatcatcc tgggtgtggt
     3481 gttcgggata ccgatcggcg tcgtctgcgc gcaatacgcc aaccgcctgc ccgatcacct
     3541 gggccgaatg ctttctctga tcggctattc ggtccccgtg ttctggctcg gcatcgtcgg
     3601 tctgctggtg ttctacgcca gactcgagtg ggtcgccggg ccggggcggc tcgataccgc
     3661 ctaccgctat tcggtgcccg aggtgacgca cctgatgttg atcgacaccg tgctggccgg
     3721 tgaatgggac gccttccgca atgccctctc gcacctggtg ttgcccgcca gcctgttggg
     3781 gctgatcgcg ctgggctaca tcgcacgcat gacccgcagc ttcctgctgt ggcaattgcg
     3841 gcaggactac accacggtgc tgcgtgtcaa gggcctgtcc gaagcggcga tcatctggcg
     3901 ccatgcgctg ttcaacgccg ccgggccggt gctggccatc gtcgcgctga cctacgccta
     3961 cctgctggaa ggcgcggtgc tgaccgaaac cgtgtttgcc tggccggggc tgggcatgta
     4021 catcaccgat tcgctgttcg cctccgatgt gccggcggtg ctcaccgcga cgctgctggt
     4081 cggcgtcatc ttcatggtcg tcaacatcgc cagcgaagtg attcaggcgg cgctggatcc
     4141 gaggtcacgt ctggcatgaa cccctcttcc accttacggc aatggctggc ttccgatacg
     4201 ccggccacac gcacacaggc caggctcggt gccatctatc ggctggcgcg ccgcttcctg
     4261 acccacccca tcggtgtgct gggtctggcc atggtggcct caatcgtgtt ggccgccggg
     4321 cttgcgccat ggctgccgct tgccgatccg atcgcccagt cgctggatca gcgcttgctg
     4381 ccaccatcgg ccgcgcactg gctgggcacc gacacgctgg gtcgcgacat cctcgcccgc
     4441 gtggtgcacg gcgcccgtcc gacgctggcc atcgtcgccc tggtgctggt gatcagcgtg
     4501 cccgtcggcc tgctggtggg cgccgctgcc gggctgtggg gcggctggat cgatgcggtg
     4561 ctgatgcgcg tggccgacgt gtttctggcg ttcccgcgcc tgatcctggc gattgccgtg
     4621 gccgccgcgc tcagcgccgg cctgtggacg gcggtgatcg ccatcgccgc cacgggttgg
     4681 cccccctacg cccgcgtggc acgcgccgag atcgccgccc tgcgccgatc cgagttcgtg
     4741 caggcggccc gcgccatcgg cgtgtcaccg ctgcggttgc tgttccggca tgcgctgccg
     4801 ctgagtctgc catcggcctt cgtgcgcgcg gcgctggacg ctccgggcat cgtgctgatc
     4861 gtggctggcc tgggctttct gggcgttggc ctgccgccgc cgcagcccga gtggggcgcc
     4921 atggtggccg aaggtcgcgc catcgtgttc gaggcgtggt gggtgtccac cctgcccggc
     4981 atcatgattc tctggctgag cctgggcttc aacttcgtcg gcgacgccat gcgtgacgtg
     5041 gtcgaccctg gcgtgaagtg agcttctcat gaaccacatc ccacgcgaca gcgcccctcc
     5101 cttgctggac atcgacaacc tgcgcatcag cttccagacc gcgcacggac gcaccgaagc
     5161 cgtgcgcggg atcagcctgc gcatgggacg tgagcgggtg gccgtggtcg gagagtcggg
     5221 ctccggcaag tcgatcacct ttcgctccct gttcggcctg ctgccggcga ccgccagcgt
     5281 gcgcgccgac cggatgcagt tgcagggcca ggacctgctg tcgctcgatc ggcgcgactt
     5341 gcgggccatt cggggccggc gcatcggcat ggtggttcag gaccccaagc agggtctgga
     5401 tcccttgatg acgattggca ggcagctgat cgaaatgctc aggctgcatc gcaaggtacc
     5461 agcggcgggc gcctaccagc gggccgtcga gctgctggcc gaggtccaca tccgcgatcc
     5521 gaagcgggtc atgggtctgt acccgcacga agtctccggc ggcatggcgc agcgcgcgat
     5581 gctggccatg attctgtcag ccgagcccga actgctgatc gccgacgaag ccacatcggc
     5641 ccttgacgcc gtggtgcgcc accgcattct ggatctgatc gaggagcagg tgcgcctgcg
     5701 cggcatgggg ctggtgctga tcagccacga cctcgatctg gtggcgcgct acgcggaccg
     5761 gatcctggtc atgtacgcgg gacgggtcat ggaagagctg cgcggcggcg aactgagcgc
     5821 cgcgcaacac ccctacacgc gcggtctgct ggcctgccgc ccctcgctcg atcacccggg
     5881 tcaggatctg ccggtgctgc aacgggaggc gtcatggctg cagtgaaccc gcccgcgctg
     5941 cgcattgacc agctcatcgt gcagcgcggc agcgcccagg tggtcaacgg cgtgtccttc
     6001 tcggttccgc atggcggcag cctgggtatc gtcggcgaat cgggttgcgg caagagcacc
     6061 atcttgcgcg ccgtcgccgg catcgacacg cagtggaccg gccggatcga gattgccggc
     6121 cggcccatcg gggttcagcg ctcgctggcc gaccgccggg cgatgcagat ggtgtttcag
     6181 gatcccctgg ctgcgctcaa ccccagccac accatcgacg agatcctgcg cgaaccgctg
     6241 atcgtgcacc gcatgcccga tcaggacgca cgcatcgtgc aggcgctgca gaccgtggcc
     6301 ctgccgctcg ccgtgcggtt ccgctttcca gggcagctgt cgggagggca gcgccagcgg
     6361 gtgtgcatcg cccgggcgct gctggtcgag ccgcaggtgc tgctgctgga cgagccgacc
     6421 tcggcgctcg atgtgtcggt gcaggccgag gtgctgaacc tgctgtcgcg cctgcgccga
     6481 gagcgccagc aggcgttcgt gctggtcagc cacgacctgc atgtggtggc ccacatgtgc
     6541 gaccagatcg cgttcatggt cggcggtcag ttcaccgatg tgctgtcacg cgatcgcttg
     6601 cgcggcgcca cgctgcatca ccccgaggca cagcggctgc tggatgccgc actgacgcac
     6661 tgaggcaggc tcgacccgac tcttcgttct gttgtttgga aagttggttt tccatttgtg
     6721 aattaactat catcacctcc atcttcacgg aacgccgcat cacatgaccc gatctgcctt
     6781 cgaccctacc gccgcgagcg gctccgctgc gccgctgctg cccctggccg gtgtgcgcgt
     6841 ggtggagttc tgcaccaccg cgtcgggccc cttctccacc atgctgctgt cggacatggg
     6901 cgccgacgtg gtgaagatcg agccacccac cggggacggc ctgcgtcagt ggccgccact
     6961 caatcagggc tacagcgaaa actttgcctc gctcaaccgc aacaagcgct cggccgtgct
     7021 caacctcaag gacccgcagg acgttgcgct ggcgcgccgg ctgatcctgg aagcggacgt
     7081 gctggtcgag aacaaccgcc ccggcgtcat ggatcgcctg gggctgggtt acgccgccct
     7141 gtcggccgaa aaaccggagt tgctgttctg ctccatctcg gcctacggcc agagcggtcc
     7201 gcgcgccagc gaaggcggct tcgacctgac gatccaggcc gcggccgggg tgatgagcgt
     7261 caccggcgaa ccggcgggtg cgcccgtcaa gtgcggcgtc ccgctgtcgg acttcgcctc
     7321 cggcctgtat gccgcgtttt ccatcgcctc catgctggcg cacgtgcgcg ccggcgggcg
     7381 cggtgcccac atcgacatcc cgatgttcgg ttgcaccctg ggcattgccg cgctgcagac
     7441 cagcgaatac ttcggcaacg gccgcagccc cggcaaactg ggctcggccc acccgcgcaa
     7501 tgcgccgtac caggcgttcc aggcgcagga cggctacttc gccatcgccg cgggcaacca
     7561 caagctgtgg ctggacgtgc tgcgcgtggt gcagatgccg gcactggccg aagacccccg
     7621 cttcctcagc accaccgacc gggcccgaaa ccagcaggcg ctcaaggaga tcctcgagga
     7681 agtgttcacc cagcggccgg tggcgcactg gatcgacgcc ttcaaccaag ccggcgtgcc
     7741 gcacgcccgc atcaacgact acgccgcggc gctggccgat ccgcagaccg tccacatggg
     7801 ctgggtgcag ccgctgacgt tgcccaacgg ccagcagacc cgcaccttcg cttcaccggt
     7861 gcgcctgaac ggtcagggct tcccgatccg ccgctcgccc cccgaactgg ggcagcacac
     7921 gcaagagatc cgcgccgaat gcgcccgatc ggaggtgaat tgatggctgc cgaatcgccc
     7981 ctgctgacct ttgtcgccgc catgacgcgc ctggtggagt ccgggccggc cgacgaggcc
     8041 aggctgctcg acggtgcccg gcccctgatg aaggccctgg tagcgcagga cgactggctg
     8101 cccgacgcca tggcggctgc gcacccccag tactaccagc agcatctgct gtacggtgac
     8161 ccgctggatc gtttttcgct ggtcagcttc gtctgggggc ctggacagaa aacgccggtc
     8221 catgaccaca cggtgtgggg catcatcggc atgctgcgtg gcgccgaatg cgcgcagcgc
     8281 tacacgcgcg atcaccaggg acgggtcacg gcctgcggcg cggaagaaac cctgcggccg
     8341 ggggacatcg acgtggtgtc tccgacgctg ggcgacatcc acgtggttcg caatggcctg
     8401 gaagaccagg tgtcgatcag catccacctg tacggcggca acatcggacg cattgcacgc
     8461 cacgtcttcg tgcccgagac agacacggtc aaggacttcg tctcgggcta ttcgaacacg
     8521 ctgtcgccca acctgtgggc caggcaagcg gcatgagcca gccagaactg ctgagccggc
     8581 gcgacggccc tgtgctgcac ctgcagctgc accggcccga ggtcggcaat gcgctgtcgg
     8641 ccgcgctggt tcaggcgctg accgaagcgg tggacgccgc cactcacgat gtcagcgtgc
     8701 gcctgctggt gctgtcgggt gcgggccgac acttctgcac cgggttcgac ctgtcggacc
     8761 tcgatcgaga gaccgacgac agcctgctgg cgcgcttcac acgggtcgag ctgctgctgc
     8821 aggccgtgca cgccgcgccg tttgccacgc tcgcgctggc gcatggccgc accatgggtg
     8881 cgggtgccga cctgtttgcc gcctgcggcg atcgctggat cgtcggcgac gccggttttg
     8941 cctttcctgg cgccgccttc ggtctggtgc tcggcacggt gcgtctgacc gaactggtcg
     9001 gcgcgcccca cgccgccgcg tggatcgaag gcggccagcg catcgacgcc gaccaggccc
     9061 tggcctgcgg tctggccaca cggcacatcg agaccgaaca gatcgatgcc gaactgggtc
     9121 ggtggggcgc ccgcgtgaac cggctcgacg cgcccaccca gcgcgccatc caggccgcca
     9181 tcgccgccgg ccgccggcca cgtggtgacg ccggagacgc ccaggacctg gcgcgcctgg
     9241 tgcgttccgc cgcccgcccc ggactgcgcg accgaatcgc cgcctaccgg gccgcccagc
     9301 gcaagggctg agccctcctt cccatctcct gccggatcga catcgtgacc tcgcccactt
     9361 ccagccacag cctcaaatcc ctgctcaacc cccgctcggt ggccgtcatc ggcgcgtccg
     9421 aagaccagac caagttcggc ggccgcctct acaagacgct gttgcagcac cactacgacg
     9481 gtgcggtcta ccccatcaat ccgacccgcg accaactgtt cgggctcaaa acctacccca
     9541 gcgtgaccga cacgccgcag gcgcccgaca tggtggtcat ggccctgccg cgcgacaagg
     9601 tcaaggacga gatcgccgcc tgcgcggcgc gcggcgccaa ggccggcatc atcatcacct
     9661 cgaagttctc cgacgccggc cccgaaggcc tggccctgga gcgcgaggtg gtggccaccg
     9721 ccgccgcgca cggcatgcga ctgatcgggc caaactgcct gggcctgatc agccctgcca
     9781 acaagctggt gctgtgctcg tcgccggcgg tcaacgtacc gcagctgatc gaggcgccga
     9841 tcggcttcat cagccagagc ggcgccctga tgggcacgct gttcgaccgc tcctacggca
     9901 tgggcatcgg gttctcgcac tgcgtgtcgg tgggcaacca ggccgacctc gaactgaacg
     9961 atttcgtcga gttcctgatc gaggacgaac gcacgcaggt gatctgcagc tatgtcgaag
    10021 gcatcaagac gccgcagcgc ttcgtcgagc tggcgcgccg ggcgcgtgcc gccggcaagc
    10081 cctggctgat ggtcaaggcc ggcgccaccg acgacgggcg ccgcgccgcc tactcccaca
    10141 ccgccagtct ggcgggcgac ttcgccgccc tcaaggcgat ctgcgaacgc gagaacgtgg
    10201 tcctgatgga cgacccgctg gacatgctga gcctggcccg ggccatggtg cgctatccgc
    10261 ggcgccaggt gcgcaacgtc gcggtcttca ccacctccgg tggcggtggc gccatcacgg
    10321 ccgaccagct ctcgcgcgct ggcgtcggcc tgagcacttt cagcgacgcc accatggccg
    10381 cgctgtccga gcactactcc gaaggtcagg gcgccaaccc gatcgacgtc ggcggccgca
    10441 agccggacgc caccagcgac atcggcgccg tcacggccga gctggcgctg gccgaccccg
    10501 gtgtggacct cggcctgatg gtgctgacca ccgcgcccga cgtgcccggc ctgacgcgcc
    10561 agatggccga cggtgccgac cgcccggcgg ccggcggcaa gccgacgctg tacgtcatgc
    10621 tgcccggccg cgtggcggca cccgcgcgcg agttcctggt gcagcgcagc ctgccctacg
    10681 tggacacgct ggccgaggcc atggccgtgc tgcgcggctg gaaggcctgg tcgacttacc
    10741 aggagccggc cgcgccggtg cgcccggccg atatggccgc cacgccggtc cccgccgccg
    10801 gcaccctggg cgaagcggcg tccaagacgc tgctgggcca ggctggcatc cccgtcaaca
    10861 tcgagcgcct ggtgcgcagc gcggacgaag ccgcagaggc ggcggccgag ctgggctttc
    10921 cgctggtgct caaggtggtc tcgcccgaca tcgcccacaa gtccgacgtc ggcggcgtgg
    10981 ccctgaacat cctcgaccct gacgacctgc gccgccgcct ggcgctgatg caggccagcg
    11041 tcgcccgcgc tgcacccagc gcccgcatcg acggcttttc actgcagcga caggaagcgg
    11101 gcgaactgga gctgatcgtc ggcgcccgcc tcgacccgca gttcggcccc caggtgctgg
    11161 taggtgccgg tggggtgctg gtggagctgc tcaaggacat cgccgtgctg cccgcccccg
    11221 tcgacgccgc cagcgcgcgc cgggcggtcg agaacctgaa gatcgccccg ctgctgcgcg
    11281 cctaccgagg tcgcgctgcc ctggacgtgg acgcggcggt cgacgtcatc gtgcgcctgg
    11341 gctggctggc gcacgatctg gccgggtctt ccgggcgcga cttcgagatc gaggtcaacc
    11401 ccctcaagct gcggcagcag ggccacggcg cggtggccgt ggatgcgcgg gcgcgcatcg
    11461 cccaggcctg attcaccgtc ttttccattc cccagcttca ggagttatcc atggcgcaaa
    11521 tcaccatcga acatcatggc aaggtgaccc tgttcaccat caaccgcccg gcgcgcaaga
    11581 acgccatctg caaggacacg gcgctggagt tgcaggccgg ctttgccgaa ttcgacgcct
    11641 ccgaacagcg cgtggcggtc atcaccggcg ccggcaacga cgccttttca tccggcgccg
    11701 acgtcagcaa cctgcccgaa ctgtggcgct gcatccccgg ctcgggcttc accaccgata
    11761 agcccatcat cgccgccacc gccggttggg tggtgggcgg cgccatggtg ctgaccatga
    11821 tgtgcgacct gatcgtcgcc gctgaaaaca ccaggttctc ctaccccgag gcacgcctgg
    11881 gcttcaccgg cggcatgatc gccgggctgg cggcgcgcat tccgcacaag gcggccatgg
    11941 acctgatgct gctgggccgc accatggacg cgcagcgcgc ctacggcatc ggcctggtga
    12001 gcgacgtggt gccgaccggc aaacagctcg aaatcgccat ggaacaggct cagaccctgg
    12061 ccgcgtcggc accgctggtg ctcaccacca tcaagcgctt cgtcaaccag cacgtgctgc
    12121 ccaagagccc ggccgaagcg atgtcggaaa ccctgcgcca gctcaacgtg gtgcgcgaaa
    12181 gcgccgacat caaggaaggc atgg
//
LOCUS       DAOQTZ010000083        12097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_5944_length_12097_cov_11.045009, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000083 DAOQTZ010000000
VERSION     DAOQTZ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12097
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5944_length_12097_cov_11.045009"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PKN51_10055"
     CDS             <1..1131
                     /locus_tag="PKN51_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85484.1"
                     /translation="DSPRARLKALLTGRALHRSHWALQDVSLELARGQCLGVVGHNGA
                     GKSTLLKLICGTLQPSQGRVERSGRVTAILELGAGFHPDFTGRQNLYFGGSLIGISHP
                     QMAELEASVLAFAEIGEAIDRPVKTYSSGMVVRLAFALVTALEPDLLIIDEALAVGDQ
                     HFQKKCIERIQSFRDHGCTILFCSHSPYHVRHLCDVALWLDHGRMMEFGSTEAVLGAY
                     EKHSRLLGGERAETAAAPLPYERGPDSALILSCDIADLGDGDPPTLHSPDLVATITVR
                     GKGAERPNIGFMIEQERGVGITSLATHEEDAAPTEVEPGVWRSTLTFPDLPLHSGDYV
                     ISVFLFDETGLVTYDLWFQFKVIRIVSPTLMPGLVRLPHRWS"
     gene            1135..2061
                     /locus_tag="PKN51_10060"
     CDS             1135..2061
                     /locus_tag="PKN51_10060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT85485.1"
                     /translation="MGLKELLDVTAAGPLPPEQARAALARGQALLAAGDRDGAATALR
                     QAALTRETQLAAHNLIESQGLPGAFAPWMGLDARIDPQDDVFGFFNGHGSWSSPLRDY
                     LADGWRTLSELMLLLERGGRTLAGFDAVLEFASGHGRFTRHLAKALGPGRVTVSDVVP
                     GAVAFSTTHFGVQGFVSAARPAQLHWPRRYGLVFVLSLFTHLPRATWSAWLARLWEAV
                     APGGVLVFTTHGALAAGRAGVTLDAEGFHFHPESESHAIDVAEYGTSYTSRGFVEAEL
                     GLLRPAPTRVDFEPAWFWAHQDAWVLQRAEAA"
     gene            2058..>2856
                     /locus_tag="PKN51_10065"
     CDS             2058..>2856
                     /locus_tag="PKN51_10065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT85486.1"
                     /translation="MSTPVTPSEYDAWMRAHEAALDAGEPGAAAAIRAAREVPATRLA
                     AHNLIERRGLDGAFAQMFGLNCRIAPRDDIFHFFAGHPTSTNPLRDYLADGWRTLAEL
                     MQLLEAVDRPLLKLASVLEFASGHGRFTRHLVKAIGPQRVTVSDVVADAVGFARDTFG
                     VQGFVSDPQPEAVPWPRRYELVFVLSLFSHLPRHSWARWLASIWRAVAPGGLLVLSTH
                     GAEAARRAGVVLDDRGFFFAASSESSAIDAQEYGTSFTSEAFVREQVA"
     assembly_gap    2857..2956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2957..3781)
                     /locus_tag="PKN51_10070"
     CDS             complement(<2957..3781)
                     /locus_tag="PKN51_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT85487.1"
                     /translation="MDRVVAVLVTYHPDRQRLAQALQRLHAQVAACVVVDNTEGGPPA
                     DAAAAGDGEWIANGRNLGIGQAQNLGIARARALGASHVLLLDQDSLAPADLVPALLRT
                     LSQAAAAHAVAAVGPLCRDVKTGATLPLIRHSGWRVRRLRPGRSDGAVAVDYLPASGT
                     LIPLHALDRVGPLREDYFIDRVDVEWCLRARHQGWTILVDPAVELQHDLGRRALRLLG
                     RTVYVGQDWRACLHVRNSLAMALRAPIALRWRIDQWLKTPLYLLFYAAVAEGGRLRQ"
     gene            complement(3792..7619)
                     /locus_tag="PKN51_10075"
     CDS             complement(3792..7619)
                     /locus_tag="PKN51_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT85488.1"
                     /translation="MAAPSPALHVYERDIDASERTSLSVLTRHIPAGARVLDLGCGSG
                     AIGRFLAARDGAGAGPIDGLTLSHDEARRAAPHYRRVEVADLESCDLPARFAPGSYDV
                     VVCADVLEHLRQPQRVMDQCRRLLAADGRALLSVPNAGYVGLVAELMAGEFRYRPEGL
                     LDDTHLRFFTRQSLRRFLADQGWTMDSLETVQRALPESEFRIAFDTLPPAVARHLLAL
                     PDALTYQFIIVSRPAPAQPLPAAAPIDTPAVAQAQFTAQLYWDDGQGMDEAHKLVTTG
                     VIGQRQQTLRFQLPAALPGLQGLKLDPADRPGILHLRSICLRSAAEPPRVAWQWSALA
                     DGTAALLASARTQQMVCGGALPGDASAAVLLTGDDPWIHLPIAADTLQQAAAAGPLLL
                     EVQLDWPMSADYLSLASVVGPLQAEQQRLREQHAADAGRLAELHQDHERLALENAHLR
                     HDRDALFAAGQSAQQQLAELHQQLHGMAEHVDNLRNLRAVRYTRLLAELVNGVRRPPG
                     RTPAPAGAAPAEADAGAPVPPPLSPTVDIIVPVYRGLRDTQVCIDAVLAGGPRTDWRL
                     IVINDCSPEPEVTAWLRDKAAAEPRITLLENEHNLGFVGTVNRGMRQSEHNDVLLLNS
                     DTEVAGDWLDRLRAAAYRQARAGTVTPFSGNATICSYPVFCADNPLPPGHSTASLDRL
                     FAQVNAGQSVPVPTGVGFCMYIRRDCLQAVGLFDEAHFGKGYGEENDFCQRAIGLGWH
                     NLHALDTYVLHTGGVSFGDSKSPREQAAMETLRRLHPGYDAQVQRFLQLDPARPARLA
                     VDWLRHTDGGRRQVVLVVQHQRGGGTERHILELAATLADTAVLVSLRPAGGQRVLLQL
                     IEQDAGRWVLSRRWAASFDLGHDGDALLRLLRDMAVSHVHYHHLLGHAPRVWTLAHEL
                     GVAYDVTCHDFYSFCTHITLTGRHGRYQMDAQGECCGGDHPPSLPETREPIDAWRIRN
                     RRFLQQARCVLAPSLDTAERMRRAMPGATLRCAPHTDILPGTLPMPQPPRLAAQEPLR
                     IVAIGALSVIKGADVLEETARLARRAGAPLEFHLIGYGYRHLQTAPGTALTVHGQYAD
                     EDLPALLQRLAPHLAWFPAQWPETYSYTLSAALQAGLPVAVPDLGAFAERVAERPWSW
                     VQAWDSSASQWLELFLRIRQQAFVDGQVQAAPPLSAPLQALRRQLGDWQYASDYLPAA
                     APAPWPEQLERARRVAAALAQAAPADAAAAPLGADRLYALALRLQRLPLLGRALRAVP
                     RSWRYRVRQLLSR"
     gene            7736..8848
                     /locus_tag="PKN51_10080"
     CDS             7736..8848
                     /locus_tag="PKN51_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT85489.1"
                     /translation="MLSLALLAFVLSLVATLWVRRLFQRWARGYDLDAPQRFHAGDTP
                     RMGGMGMLLGWTLALAALPLLQGIGMAGNIDGTRLHFSLWLLMLLPAFAGGVVEDLTQ
                     RLRVSSRMLLTLISGLLAWALLGVSVPQLGFAWVDGLWGAWPGLGMALAVLAVTGLPH
                     AFNIIDGYNGLAGSVALVVCLALAHVALQVGDRELAAVAVTLAAATAGFLVWNYPRGL
                     IFAGDAGAYLWGLLIAVLSLLLVQRHPIVSPWFPMLLLIYPVWETVFSIYRKLARGDS
                     PGMADALHLHQLVYRRLVRSVLHEDEAQSLLKRNNRTTPYLWGFMVLSVAPALLFWRY
                     SAVLLAFCVLFAVCYVWAYLSLIRFRVPRVLQPPRE"
     gene            8900..9793
                     /gene="argB"
                     /locus_tag="PKN51_10085"
     CDS             8900..9793
                     /gene="argB"
                     /locus_tag="PKN51_10085"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNT85490.1"
                     /translation="MTTHTTALSPRDKAEILAQALPYIRQFHGKTMVIKYGGNAMTDP
                     ALQQDFAEDVVLLKLVGIHPVVVHGGGPQIEQALNRLGKKGEFIQGMRVTDAETMEVV
                     EWVLAGEVQQDIVGLIHQAGGKAVGLTGRDGGLIRARKLKLLDPKDPAIEHDVGQVGD
                     IVAIDAAVVRALQDDQFIPVVSPIGFGERNESYNINADVVAARLAMELQAEKLLMLTN
                     IPGVLDKAGQLLAELTPRQIDALVDDGTISGGMIPKISGALDAARSGVKAVHIIDGRV
                     PHALLLEVLGDQPFGTMIRAQ"
     gene            9931..10575
                     /gene="slmA"
                     /locus_tag="PKN51_10090"
     CDS             9931..10575
                     /gene="slmA"
                     /locus_tag="PKN51_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HNT85491.1"
                     /translation="MPTAYDPSTDHSDHAGAAPAAGRKRPKPGERREQILQALAQMLQ
                     QPGAERVTTAALAARLGVSEAALYRHFASKAQMFEALIDFIESSVFGLIGQISERETD
                     GRARAARIVAMVLQFGERNPGMARVMAGDALVLEHERLQQRMNQWFDKLEAALRQSLR
                     EAAATPGTLTPDADAQVAAAVLTAFVAGRLQRFARSGFRRAPTEQGDACIARIL"
     gene            10651..>10862
                     /locus_tag="PKN51_10095"
     CDS             10651..>10862
                     /locus_tag="PKN51_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"
                     /protein_id="HNT85492.1"
                     /translation="MKLLRCGPPGREKPALLDADGVLRDLSALLPDIGPAQLSDTALA
                     RLARLRPQHLPVVRGRPRIGCPVAGIG"
     assembly_gap    10863..10962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11006..11635
                     /locus_tag="PKN51_10100"
     CDS             11006..11635
                     /locus_tag="PKN51_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT85493.1"
                     /translation="MPAAETSVEPRPRPLPKGQQTKVAIVDAALRMAAQVGLEGLSIG
                     SLAETMGMSKSGVFAHFGSRDELQISVIREYYARFEAQVFQPAMAQPRGLPRVRALFE
                     NWMRFTSAELDSGCIFISGAVEFDDRPGPVRDALAEAVDAWIEAMTRAVAQACEQGHL
                     AADADPRQISFEIHALILALHYEARFMRRPGSMERAEQGFRNILARAGA"
     gene            11677..>12097
                     /locus_tag="PKN51_10105"
     CDS             11677..>12097
                     /locus_tag="PKN51_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HNT85494.1"
                     /translation="MPSYTPPLRDMQFVLHEVFHVADELKAMPTHAETDADTINAVLE
                     EAGKFAAGVAFPLNVSGDAEGCRLDAATHAVTTPKGFKEAYAAYVEGGWPALSCDPQY
                     GGQGLPHVVNQCLYEMLNSANQAWTMYPGLSHGAYEAL"
BASE COUNT         1612 a         3999 c         4489 g         1797 t
ORIGIN      
        1 gactcgccgc gcgcgcgcct gaaggcgctg ctgaccgggc gcgccctgca ccgcagccac
       61 tgggcgctgc aggacgtcag cctggagctg gcgcgcggcc agtgcctggg cgtggtgggc
      121 cacaacggcg cgggcaagag cacgctgctg aagctgatct gcggcacgct gcagccgagc
      181 cagggccgcg tcgaacgcag cgggcgggtg accgccatcc tggagctggg tgcgggcttt
      241 caccccgatt tcacggggcg ccagaacctg tacttcggtg gcagcctgat cggcatttcg
      301 cacccgcaga tggccgagct ggaggcttcg gtgctggcct ttgccgagat cggcgaggcg
      361 atcgaccggc cggtcaagac ctactcgtcc ggcatggtgg tgcggctggc gttcgcgctg
      421 gtgacggcgc tggagccgga cctgctgatc atcgacgagg cgctggcggt gggtgaccag
      481 cactttcaga agaagtgcat cgagcgtatc cagtccttcc gcgaccatgg ctgcaccatc
      541 ctgttctgct cgcacagccc ctaccacgtg cgccacctgt gcgatgtggc gctgtggctg
      601 gaccatgggc ggatgatgga gttcggcagc accgaggcgg tgctgggcgc ctatgaaaag
      661 cattcccgcc tgctgggcgg cgagcgcgcc gagaccgccg cggcgccgct gccttacgag
      721 cgcggccccg attcggccct gatcctgtcg tgcgacatcg ccgacctggg cgacggcgat
      781 ccgcccacgc tgcacagccc cgatctggtg gccaccatca cggtgcgcgg caagggtgcc
      841 gagcggccca acatcggctt catgatcgag caggagcgcg gcgtgggcat cacctcgctg
      901 gccacccacg aggaagacgc cgcgccgacc gaggtcgagc cgggggtgtg gcgctcgacg
      961 ctgacgtttc ccgacctgcc gctgcacagc ggcgactacg tgatcagtgt gttcctgttc
     1021 gacgagaccg ggttggtgac ctatgacctg tggtttcagt tcaaggtgat tcggatcgtt
     1081 tcaccgacac tgatgccggg gctggtgcgg ctgccgcacc gctggagttg agcgatgggc
     1141 ttgaaggaat tgctggatgt caccgccgcc ggcccgctgc cgcccgagca ggcgcgcgcg
     1201 gcgctggcgc gtgggcaggc gctgctggcc gccggggatc gcgacggcgc cgccaccgcg
     1261 ctgcgccagg cggcgctgac gcgtgagacg cagctggcgg cgcacaacct gatcgaatcg
     1321 cagggtctgc cgggtgcgtt tgcgccctgg atggggctgg atgcgcgcat cgacccgcag
     1381 gacgacgtgt tcggcttctt caatgggcac ggcagctggt ccagcccgct gcgcgactac
     1441 ctggccgatg gctggcgcac gctgtcggag ttgatgctgc tgctcgagcg cggcgggcgc
     1501 acgctggcgg gcttcgacgc ggtgctggag tttgccagtg gccacggccg cttcacgcgg
     1561 cacctggcca aggcgctggg gccggggcgc gtgacggtgt cggacgtggt gccgggcgcg
     1621 gtggcgtttt cgaccacgca tttcggcgtg cagggctttg tgtcggcggc gcggccggcg
     1681 cagctgcact ggccgcgccg ctacgggctg gtgttcgtgc tgtcgctgtt cacgcatctg
     1741 ccgcgcgcca cctggagcgc ctggctggcg cggctgtggg aggcggtggc gccgggcggc
     1801 gtgctggtgt tcaccacgca cggagccctg gcggccgggc gcgccggcgt gacgctcgat
     1861 gccgagggct ttcactttca ccccgaaagc gaatcgcacg cgatcgacgt cgcggaatac
     1921 ggcaccagct acaccagccg tggcttcgtc gaggccgagc ttggcttgct caggccggcg
     1981 ccaacgcggg tcgatttcga gccggcctgg ttctgggccc accaggacgc ctgggtgctg
     2041 cagcgtgcgg aggccgcatg agcacgccag tgacaccaag cgaatacgac gcctggatgc
     2101 gggcgcacga agcggcgctg gacgcgggcg agccgggcgc ggcggcggcg attcgcgccg
     2161 cacgcgaggt gccggccacg cggctggcgg cgcacaacct gatcgagcgg cgtgggctgg
     2221 atggggcgtt tgcccagatg ttcggtctga actgccgcat tgcgccacgc gacgacatct
     2281 ttcacttttt tgccggccac ccgacctcga ccaatccgct gcgcgactac ctggccgacg
     2341 gctggcgcac gctggctgag ctgatgcagc ttctggaagc ggtggatcgg ccgctgctga
     2401 agctggcatc ggtgctggag tttgccagtg gtcacgggcg cttcacgcgc cacctggtga
     2461 aggcgatcgg gccgcagcgg gtgacggtga gcgatgtggt ggccgacgcg gtgggctttg
     2521 cgcgcgacac gtttggcgtg caaggcttcg tgtcggaccc gcagcccgag gcggtgccgt
     2581 ggccgcgccg ctacgagctg gtgttcgtgc tgtcgctgtt cagccacctg ccgcgccaca
     2641 gctgggcgcg ctggctggcc agcatctggc gggcggtggc gccgggcggg ctgctggtgc
     2701 tgtcgaccca cggcgcggag gccgcgcgcc gcgccggcgt ggtgctggat gaccgtgggt
     2761 tcttttttgc cgcctccagc gagtccagcg ccatcgatgc gcaggagtac ggcaccagct
     2821 tcaccagcga agccttcgtg cgcgaacagg tggcgannnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnctgg cgcagccggc cgccttcggc cacggcggca tagaacagca
     3001 ggtacagcgg ggtcttgagc cactggtcga tgcgccagcg cagcgcgatg ggggcgcgca
     3061 gcgccatggc gagcgagttg cgcacgtgca ggcaggcccg ccagtcctgc cctacgtaga
     3121 ccgtgcgccc cagcagccgc agcgcgcgcc ggcccaggtc gtgctgcagc tcgaccgcgg
     3181 ggtcgaccag gatggtccag ccctggtggc gggcgcgcag gcaccattcg acgtcgacgc
     3241 ggtcgatgaa gtagtcctcg cgcagcgggc ccacgcggtc cagcgcgtgc agcgggatca
     3301 gggtgccgga ggcgggcagg tagtcgaccg ccaccgcgcc gtcgctgcgg ccggggcgca
     3361 ggcggcgcac gcgccagccg ctgtggcgga tcagcggcaa cgtggcgccg gtcttgacgt
     3421 cgcggcacag cggcccgacg gcggccaccg cgtgggctgc ggcggcctgg ctgagcgtgc
     3481 gcagcagtgc cggcaccaga tcggctggcg ccaggctgtc ctggtccagc agcagcacat
     3541 ggctggcgcc cagtgcgcgc gcgcgggcga tgcccaggtt ctgcgcctgg ccgatgccca
     3601 ggttgcggcc gttggcgatc cattcgccgt cgccggccgc ggcggcgtcc gctggcgggc
     3661 cgccttcggt gttgtcgacc accacgcagg ccgccacctg agcgtgcagg cgttgcagcg
     3721 cctgcgccag gcgctggcgg tcggggtggt aggtgacgag cacggccacc acgcggtcca
     3781 tgggcggccc ttcagcggct gagcagctgg cgcacgcggt agcgccagga gcgcggcacg
     3841 gcccgcagtg cccggcccag cagcggcagg cgctgcaggc gcagggccag ggcgtacagg
     3901 cggtccgcgc cgagtggcgc tgccgccgcg tcggccggcg cggcctgggc cagcgccgcg
     3961 gccacgcggc gggcgcgttc gagctgctcg ggccagggcg ccggcgccgc cgccggcagg
     4021 tagtcgctgg cgtactgcca gtcgcccagt tgccggcgca gcgcctgcag cggcgcggac
     4081 agcggcgggg cggcctgcac ctgcccgtcg acgaacgcct gctgccggat gcgcaggaac
     4141 agttcgagcc actgcgaggc gctgctgtcc caggcctgca cccaactcca ggggcgctcg
     4201 gccacccgtt cggcaaaggc gccgaggtcg ggcacggcga ccggcagccc ggcctgcagg
     4261 gcggcgctga gggtgtagct gtaggtttcc ggccactgcg ccgggaacca cgccaggtgc
     4321 ggcgccagcc gctgcagcag ggccggcagg tcttcgtcgg cgtactggcc gtgcacggtg
     4381 agggcagtgc cgggggcggt ctgcaggtgg cgatagccgt agccgatcag gtgaaattcg
     4441 agcggtgcgc cggcgcgccg cgccaggcgt gcggtttctt ccagcacgtc ggcgcccttg
     4501 atgacgctga gcgcgccgat ggcgacgatg cgcagcggct cctgggccgc cagtcgcggc
     4561 ggctggggca tgggcagggt gccgggcagg atgtcggtat ggggcgcgca gcggagggtg
     4621 gcgcccggca tggcgcgacg catgcgctcg gcggtgtcca ggctgggcgc gagcacgcaa
     4681 cgcgcctgct gcagaaagcg gcggttgcgg atgcgccagg cgtcgatcgg ctcgcgggtc
     4741 tcgggcagcg agggcgggtg gtcgccgccg cagcattcgc cctgcgcgtc catctggtag
     4801 cggccgtgcc tgccggtcag ggtgatgtgg gtgcagaagc tgtagaagtc gtggcaggtg
     4861 acgtcgtagg ccacgcccag ctcgtgcgcc agcgtccaga cgcgcggcgc gtggccgagc
     4921 aggtggtggt agtgcacgtg gctgaccgcc atgtcgcgca gcaggcgcag cagggcgtcc
     4981 ccgtcgtgcc cgagatcgaa actggcggcc cagcgccgcg acagcaccca gcggcccgcg
     5041 tcctgctcga tcagctgcag cagcacccgc tgcccgccgg ccgggcgcag cgacaccagg
     5101 acggcggtgt cggccagcgt ggcggccagc tccagaatgt ggcgctcggt gccgccgcca
     5161 cgctggtgct gcaccaccag caccacctgg cggcgcccgc cgtcggtgtg gcgcagccag
     5221 tcgacggcca ggcgcgccgg gcgcgccggg tcgagctgca ggaagcgctg gacctgggcg
     5281 tcgtagccgg ggtgcaggcg gcgcagggtt tccatcgcgg cctgctcgcg cgggcttttg
     5341 ctgtcgccga agctgacgcc gccggtgtgc agcacgtagg tgtcgagcgc gtgcaggttg
     5401 tgccagccca ggccgatggc gcgctggcag aagtcgtttt cttcgccgta gcccttgccg
     5461 aaatgcgctt cgtcgaacag gcccacggcc tgcaggcagt cgcgccggat gtacatgcag
     5521 aagccgacgc cggtgggcac cggtacgctc tggccggcgt tgacctgggc gaacagccgg
     5581 tccaggctgg ccgtgctgtg gccgggcggc agcgggttgt cggcgcagaa caccgggtag
     5641 ctgcagatgg tggcgttgcc ggagaacggc gtcaccgtgc cggcccgcgc ctgtcggtag
     5701 gcggcggcgc gcaggcgatc gagccagtcg ccggccactt cggtgtcgct gttgagcagc
     5761 agcacgtcgt tgtgttcgga ctggcgcatg ccgcggttga cggtgccgac gaagcccagg
     5821 ttgtgctcgt tttcgagcag cgtgatgcgc ggctcggcgg ccgccttgtc gcgcagccag
     5881 gcggtgacct cgggctcggg gctgcagtcg ttgatgacga tcaggcgcca gtcggtgcgc
     5941 gggccgccgg ccagcacggc gtcgatgcac acctgggtgt cgcgcaggcc gcggtagacc
     6001 ggcacgatga tgtccaccgt ggggctgagt ggcggcggca cgggggcgcc ggcgtcggcc
     6061 tcggccgggg cggcgccggc gggcgccggg gtgcggccgg gtgggcgacg cacgccgttg
     6121 accagctcgg ccagcaggcg ggtgtagcgc acggcgcgca ggttgcgcag gttgtccacg
     6181 tgctcggcca tgccgtgcag ttgctggtgc agctcggcca gctgctgctg cgcgctctgg
     6241 ccggcggcga acagcgcgtc gcggtcgtgc ctgaggtggg cgttttccag cgccaggcgc
     6301 tcgtggtcct ggtggagttc ggcgaggcgc ccggcgtcgg cagcgtgctg ctcgcgcagc
     6361 cgctgctgct cggcctgcag cgggccaacc acgctggcca ggctcaggta gtcggccgac
     6421 atcggccagt cgagctgcac ttccagcagc agcgggccgg ccgccgcggc ctgctgcagg
     6481 gtgtcggcag cgatgggcag gtggatccag gggtcgtcac cggtcagcag cacggccgcg
     6541 ctggcgtcgc ccggcagcgc gccgccacac accatctgct gcgtgcgtgc cgaagccagc
     6601 agggcggccg tgccatcggc cagggcgctc cactgccagg ccacgcgcgg cggctcggcg
     6661 gccgagcgca ggcagatcga gcgcaggtgc aggatgccgg gccggtcggc cggatcgagc
     6721 ttgagcccct gcaggcccgg cagcgctgcc ggcagctgaa agcgcagggt ctgttggcgc
     6781 tggccgatga cgccggtggt caccagcttg tgcgcttcat ccatgccctg accgtcgtcc
     6841 cagtacagct gggccgtgaa ctgcgcctgc gccacggcag gggtgtcgat cggcgccgcg
     6901 gcgggcagcg gctgggccgg cgccggtcgg ctgacgatga tgaactggta ggtcagcgcg
     6961 tccggcaggg ccagcaggtg gcgcgccacg gccggcggca gggtgtcgaa ggcgatgcgg
     7021 aattcggact ccggcagcgc gcgctgcacg gtttccaggc tgtccatggt ccagccctgg
     7081 tcggccagaa agcgccgcag cgactggcgg gtgaagaagc gcaggtgggt gtcgtccagc
     7141 aggccctcgg gccggtagcg gaattcgccc gccatcagct cggccaccag gcccacgtag
     7201 ccggcgttgg ggacggacag cagggcgcgg ccgtcggcgg ccagcaaccg gcggcattgg
     7261 tccatcacgc gctgcggctg gcgcaggtgc tccagcacgt cggcgcagac gacgacgtcg
     7321 tagctgcccg gtgcgaaccg ggcgggcagg tcgcaggatt cgaggtcggc cacctcgacg
     7381 cggcggtagt ggggcgcggc gcgccgggct tcgtcgtggc tgagggtcag gccgtcgatc
     7441 gggccggcgc cggcgccatc gcgcgcggcc agaaagcgcc cgatggcgcc gctgccgcag
     7501 cccagatcga gcacgcgggc gccggcgggg atgtggcggg tcagcaccga cagggaggtg
     7561 cgctcgctgg cgtcgatgtc gcgttcgtag acgtgcagcg cgggggaggg ggcggccatg
     7621 cgatcggcag ttgggtaccg gctgttcgcg cgcgcgaacc gggacgacgg atgctaccat
     7681 gcaaggcttt ttgcgcacac cgcgccgcaa cgggccctga accactcgtc actgcatgct
     7741 gtccctggcc ctgcttgcct tcgtgctgtc tctggtggcc acgctgtggg tgcgcagact
     7801 gtttcagcgc tgggccaggg gttacgacct cgacgcgccc cagcgttttc atgccggtga
     7861 cacaccccgc atggggggga tgggcatgct gctgggctgg acgctggcgc tggcggcgct
     7921 gccgctgctg caagggatcg gcatggcggg caacatcgac ggcacccgac tgcacttctc
     7981 gctgtggctg ctgatgctgc tgcccgcctt tgccggcggc gtggtggaag acctgaccca
     8041 gcgcctgcgg gtgagttcgc gcatgctgct gaccctgatt tcgggcctgc tggcctgggc
     8101 gctgctgggg gtgtcggtgc cgcagctggg tttcgcctgg gtcgatggtc tgtggggcgc
     8161 ctggccgggg ttgggcatgg cgctggcggt gctggccgtg accgggctgc cgcatgcctt
     8221 caacatcatc gacggctaca acggcctggc cggttcggtg gcgctggtgg tgtgcctggc
     8281 gctggcgcat gtggcgctgc aggtgggcga tcgcgagctg gccgcggtgg ccgtgacgct
     8341 ggcggcggcg acggcggggt ttctggtctg gaactacccg cgcggcctga tctttgccgg
     8401 cgatgccggg gcctacctgt ggggcctgct gatcgccgtg ctgagcctgc tgctggtgca
     8461 gcgccacccc atcgtgtcgc cctggtttcc gatgctgctg ctgatctacc cggtgtggga
     8521 gacggtgttc tcgatctacc gcaagctggc gcgcggcgac tcgcccggca tggccgacgc
     8581 gctgcacctg caccagctgg tgtaccggcg gctggtgcgc agcgtgctgc acgaggacga
     8641 ggcgcaaagc ctgctcaagc gcaacaaccg caccacgccg tacctgtggg gcttcatggt
     8701 gctgagcgtg gcgccggcgc tgctgttctg gcgctacagc gcggtgctgc tggcgttctg
     8761 cgtgctgttc gccgtgtgct acgtgtgggc ctacctgtcg ctgatccgct tcagggtgcc
     8821 gcgcgtgctg cagccgccgc gcgagtgaca accctatgat cgagcttttg caccttgctg
     8881 ccgtttcgcg ccacctgcca tgaccaccca caccaccgcc ctttctccgc gtgacaaggc
     8941 cgaaatcctg gcccaggcgc tgccctacat ccgccagttt catggcaaga ccatggtcat
     9001 caagtacggc ggcaacgcca tgaccgaccc ggcgttgcag caggactttg ccgaggacgt
     9061 ggtgctgctc aagctggtgg gcatccaccc ggtggtggtg cacggcggcg ggccgcagat
     9121 cgagcaggcc ctgaaccgcc tgggcaagaa gggcgagttc atccagggca tgcgcgtgac
     9181 cgacgccgaa accatggagg tggtcgagtg ggtgctggcc ggcgaggtgc agcaggacat
     9241 cgtgggcctg atccaccagg ccggcggcaa ggcggtgggc ctgacggggc gcgacggcgg
     9301 cctaattcgg gcgcgcaagc tcaagctgct ggaccccaag gacccggcca tcgagcacga
     9361 cgtgggccag gtgggcgaca tcgtggcgat cgacgcggcg gtggtgcgcg ccctgcagga
     9421 cgaccagttc atcccggtgg tcagcccgat cggctttggc gaacgcaacg agagctacaa
     9481 catcaacgcc gacgtggtgg cggcgcgcct ggccatggag ctgcaggccg aaaagctgct
     9541 gatgctgacc aacatcccgg gcgtgctgga caaggccggc cagctgctgg cggagctgac
     9601 gccgcgccag atcgatgccc tggtggacga cggcaccatc tccggcggca tgatccccaa
     9661 gatcagcggc gcactggacg ccgccaggag cggcgtgaag gcggtgcaca tcatcgacgg
     9721 ccgggtgccg cacgcgctgc tgctggaggt gctgggcgac cagcccttcg gcaccatgat
     9781 ccgcgcccag tgagccgcag ccgccttcgg gcggcgctgg tggcgagcgc tcacttggcg
     9841 gctctgtggc gcggagcaaa cgccggcgca atcaggcttg gaccgcgcaa ggccttgtgc
     9901 gagaatcatt tggaaacaaa tgatgccgcc atgcccaccg cctacgatcc ttcgaccgac
     9961 cactccgacc acgccggggc ggcccctgcc gccgggcgca agcgccccaa gccaggcgag
    10021 cggcgcgagc agatcctgca ggcgctggcg cagatgctgc agcagcccgg cgccgagcgc
    10081 gtgaccaccg ccgccctggc ggcccgcctg ggggtgagcg aggcggcgct gtaccgccac
    10141 ttcgccagca aggcgcagat gttcgaagcg ctgatcgact tcatcgaaag cagcgtgttc
    10201 ggcctgatcg gccagatcag tgagcgcgag accgacggcc gagcgcgcgc ggcccgcatc
    10261 gtggcgatgg tgctgcagtt cggcgaacgc aaccccggca tggcgcgcgt gatggcgggc
    10321 gacgcgctgg tgctggagca cgaacgcctg cagcagcgca tgaaccagtg gttcgacaag
    10381 ctggaggcgg cgctgcgcca gagcctgcgc gaggcggcgg cgacccccgg cacgctgacg
    10441 ccggacgccg acgcgcaggt cgcggcggcg gtgctgaccg ccttcgtggc ggggcgactg
    10501 cagcgctttg cgcgttccgg ctttcggcgt gcgccgaccg agcagggcga cgcctgcatc
    10561 gccagaatac tataaaaaca gtagctcctg gcgcttgcct gacaagcgcc agggcctgat
    10621 tttgatctta tcgatccttc ggggtacctg atgaagctgc tgcgctgcgg cccgccgggc
    10681 cgcgaaaaac cggccctgct ggacgccgac ggcgtgctgc gcgatctgtc ggcgctgctg
    10741 cccgacatcg gcccggcgca gctgtcggac accgcgctgg cgcgcctggc gcggctgcgc
    10801 ccgcagcacc tgccggtggt gcgcggccgg ccacgcatcg gctgtccggt ggcgggcatc
    10861 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaatagaa cgaccgttcg
    10981 ttttattctg gatatgtccc gtcccatgcc tgccgctgag acctcggtcg agccgcgccc
    11041 gcgtccgctg cccaagggcc agcagaccaa ggtggccatc gtcgatgccg ccctgcgcat
    11101 ggccgcccag gtggggctgg aggggctgtc catcggctcg ctggccgaga ccatggggat
    11161 gagcaagtcg ggcgtgttcg cgcatttcgg ttcgcgcgac gagctgcaga tttccgtgat
    11221 ccgcgaatac tacgcccgct tcgaggccca ggtgttccag cccgccatgg cgcagccgcg
    11281 cggtctgccg cgggtgcgtg cgctgttcga gaactggatg cgctttacca gcgccgaact
    11341 cgattcaggc tgcatcttca tcagcggcgc ggtcgagttc gatgaccgcc ccggcccggt
    11401 gcgcgatgcc ctggccgagg cggtcgacgc ctggatcgag gccatgaccc gcgccgtggc
    11461 ccaggcctgc gagcaaggcc acctggccgc cgacgccgac ccgcgccaga tcagcttcga
    11521 gatccacgca ctgatcctgg ccctgcacta cgaagcacgc ttcatgcgcc gccccggctc
    11581 catggagcgc gccgagcagg gctttcgcaa catcctcgcg cgcgccggcg cctgaggttt
    11641 tctcacccac catcgtccat caggagaaac gccgccatgc ccagctacac cccgcccctg
    11701 cgcgacatgc agttcgtgct gcatgaagtc ttccacgtcg ccgacgaact gaaggccatg
    11761 cccacgcatg ccgagaccga cgccgacacc atcaacgccg tgctggaaga ggccggcaag
    11821 ttcgccgccg gggtggcgtt tccgctcaac gtcagcggcg acgccgaggg ctgccgcctc
    11881 gacgccgcca cgcacgccgt gaccacgccc aagggcttca aggaggccta cgccgcctac
    11941 gtcgaaggcg gctggccggc gctgtcgtgc gacccgcagt acggtggcca gggcctgccg
    12001 cacgtggtca accagtgcct gtacgagatg ctcaacagcg ccaaccaggc ctggaccatg
    12061 taccccggcc tgtcgcacgg cgcctacgaa gccctgc
//
LOCUS       DAOQTZ010000084        11848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_6096_length_11848_cov_13.437209, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000084 DAOQTZ010000000
VERSION     DAOQTZ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6096_length_11848_cov_13.437209"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKN51_10110"
     CDS             complement(<1..1088)
                     /locus_tag="PKN51_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNT85495.1"
                     /translation="MSSTPSPNWQKDIDHVVHPYSNLDAHDRRGPVVITRGDGCYVED
                     ENGKRYLEGMSGLWCASLGFSEQRLIDAAMAQFRTLPYYHLFNHRSHPPGIALAEALT
                     AIAPAGLNHVLFANSGSEANDAAVKLVWYYNNQLGRPSKKKIISRINGYHGVTVASGS
                     LTGLVHVHRDFDLPIPQVLHTDCPSHYHFALPSETEAQFAERLANNLEQLILKEGPDT
                     VAAFIAEPINGSGGVIVPPAGYFDRVQAVLKKYDILFIVDEVICGFGRTGQMFGSDSF
                     QLKPDMMTVAKGLSGAYQPISGLLITDQIHEALKAGSRKLGAFAHGLTYAAHPVAAAV
                     ALETIRLYQERRIVEHVQALSPYFFQRL"
     gene            complement(1252..>1794)
                     /locus_tag="PKN51_10115"
     CDS             complement(1252..>1794)
                     /locus_tag="PKN51_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT85496.1"
                     /translation="PSWRLDHVTLAMPAPGGADAYQRFFGAPVVCSAAYTGLHFAAEV
                     LECPIPTADLERFRNFCAQLELRMPNQATPGLLGDALRTWMSYAASEGRATLEHAASD
                     FGVTPRTLQRRLKEQGVGFQDLLAQVRMQTACRLLTESRLPVTCIAEQLGFSETSAFT
                     RAFRSHARVSPRAYRQAAAH"
     assembly_gap    1795..1894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1895..2152)
                     /locus_tag="PKN51_10120"
     CDS             complement(<1895..2152)
                     /locus_tag="PKN51_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85497.1"
                     /translation="MTTRLLVGAISGEATRLATHVLHRLLSRPVPLPARPWPWAPGAE
                     RERQDDPQPLLGFTRLLSHAAQQSGRQQLGLEMAAFDAEPPQ"
     gene            complement(2444..3709)
                     /locus_tag="PKN51_10125"
     CDS             complement(2444..3709)
                     /locus_tag="PKN51_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT85498.1"
                     /translation="MSAAATPSDASLIDSRTAAWRLLVVVLLVTLGNSAMYVVSVVLP
                     AVQAEFGIGRAGASLPYTLMMVCLGLGGLWTGLWADRWGIARVLWLGAAAVGAGYLLA
                     ALSGNVWLFGLAHGLLGLLGGAATFAPLMADTGLWWHRRRGIAMAICASGNYIAGTLW
                     PPLAQWGITHYGWRPTYVALGLVCGVGMALLALAMRRRPPALSAHPASHGAAAARATP
                     WPPERPFGLTPAQAQWLLCVAGVACCVAMSMPQVHIVAYCSDLGFGAARGAEMLSLML
                     ASGIVSRLVSGWICDHIGGIRTLLLGSALQGVALLLFLPVDGLVPLYVVSALFGLFQG
                     GIVPSYAIIVREHFPAREAGARTGAVIMATLLGMALGGWLSGWVFDLTGSYRAAFLNG
                     IGWNLLNLAIASWLLWRTRGPGLRLAATP"
     gene            complement(3724..4041)
                     /locus_tag="PKN51_10130"
     CDS             complement(3724..4041)
                     /locus_tag="PKN51_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNT85499.1"
                     /translation="MTTSHIANVTLTTQANVYFDGKCVSHGFQQADGSRASVGVILPA
                     TLSFNTGAAEVMECVAGGCEYRLAGETSWKSSGPGECFSIPANSSFEIRVAEVYHYIC
                     RFG"
     gene            4105..4935
                     /locus_tag="PKN51_10135"
     CDS             4105..4935
                     /locus_tag="PKN51_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNT85500.1"
                     /translation="MNPPRTRFVLIETSHAGNVGAVARAMKVMGFDDLVLVRPRWPDV
                     LSRPEAIERASGAGDVLARARCVATLDEALDGMTHLCATAMTPRDFGPPTRAPRAHFD
                     LLLKQELSAHVPQAPALDFDLEAGQGVALLFGSERYGMRNEDVYRCHVALSIPTDPAY
                     GSLNLGAAVQLIAYDWRQALAARFGGFDIAPAGAAPRHADAAQLGAMLAHWEQALVAI
                     GFLDPAAPKKLMPRLQQLFNRAQPTLEEVHILRGIARAMQRAASSGQQDDGAPAPRPR
                     "
     gene            4949..5719
                     /gene="cysE"
                     /locus_tag="PKN51_10140"
     CDS             4949..5719
                     /gene="cysE"
                     /locus_tag="PKN51_10140"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNT85501.1"
                     /translation="MLSRIRSYTQAILERDPAARSVWDVVFSYPGFHALVLHRLAHPL
                     WNAGWHWLARWISHWGRFLTGIEIHPGAKIGERVFIDHGMGVVIGETAEIGDDCTIYQ
                     GVTLGGTSLTKGSKRHPTLGKGVVVGASSQILGGFTVGDGAKIGSSAVVTKPVPAGAT
                     AVGNPARIILADDQTRREEAAAKLGFSAYGVTQSDDPVSQALRGLIDGTATHEHQIAL
                     LWQAVEKLACQIDPDCVPGDASRQETFEADKLNQLVGK"
     gene            complement(5742..5999)
                     /locus_tag="PKN51_10145"
     CDS             complement(5742..5999)
                     /locus_tag="PKN51_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNT85502.1"
                     /translation="MTRFTPMQTPSSPWRRLALAAALLAGLTSAGCASHPTNAQIGTG
                     VGAVVGGATGHVLFGGPVGTIGGAAAGALIGHEVGKNQRRR"
     gene            complement(6216..6809)
                     /gene="mog"
                     /locus_tag="PKN51_10150"
     CDS             complement(6216..6809)
                     /gene="mog"
                     /locus_tag="PKN51_10150"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNT85503.1"
                     /translation="MNTCDPVTIGIVSISDRASSGAYEDKGLPALQEWLGRALKNPIR
                     FQPRLIPDEQVEISRTLIELVDAGCALVLTTGGTGPAPRDVTPEATLAVAEKLMPGFG
                     EQMRQISLAFVPTAILSRQVAVIRGQSLIINLPGQPKAIAETLEGLKDADGRPQVHGI
                     FAAVPYCIDLIGGPYLETDETVCKAFRPKNAQRPVRA"
     gene            complement(6843..7523)
                     /gene="yjgA"
                     /locus_tag="PKN51_10155"
     CDS             complement(6843..7523)
                     /gene="yjgA"
                     /locus_tag="PKN51_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNT85504.1"
                     /translation="MSRKPRKGYYVRGQFVAEGSELDEELRREQLADGPSKTELKAQS
                     AELQALGEQLLSLRADLLQPLALPARLLEALQELQRITNFEGKRRQSQYVGKLMRQLP
                     GEDVAAIRAALHEQRQGAARDTLRLHAAERWRERLVADDAALSAWIGQFPDSDAQQLR
                     ALVRQARKDAAAPADAAAARARGQAARHGRAWRELFQLIQAALARAEAAGPNPDDEVP
                     YSDDTRAG"
     gene            7566..8939
                     /gene="pmbA"
                     /locus_tag="PKN51_10160"
     CDS             7566..8939
                     /gene="pmbA"
                     /locus_tag="PKN51_10160"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HNT85505.1"
                     /translation="MQDTSQPQAGFSYSRARFEELVDTAIAHARALGATDAGAEASEG
                     VGLSVGVRMGELETVERNRDKSLGVTVYLGQRRGNASTSDFSRAAIERTVQAAYDIAR
                     FTAEDPAAGLPDPADVAQPHEQRDLDLFHPWSIDSEQAAALALACERAALAVDRRITN
                     SEGASVSAQQSHFFSAHTHGFRGGYASSRHSLSVAPIAGRGEDMQRDYWYSSMRDAAE
                     LAAPEAVGRYAAERALSRLGSRKIATGECPVLFESPLAAGLLGSFVQAVSGGALYRRT
                     SFLLDSLGKPVFADHIDVGEDPFVRSGKGSSPFDEEGVRVQPRQVVEAGRLQGYFLST
                     YSARKLGLKTTGNAGGSHNLTLSSRRTRAGDDLDAMLRQLGTGLFVTELMGQGVNYVT
                     GDYSRGASGFWVENGRIAYPVQEITIAGNLRDMFRGIQAVGADTYTYGAKTVGSILID
                     RMTVAGN"
     assembly_gap    9143..9242
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9243..10915)
                     /locus_tag="PKN51_10165"
     CDS             complement(<9243..10915)
                     /locus_tag="PKN51_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85506.1"
                     /translation="MTLNPLHSGWRRWCAGALLLLAPLLGWAQADPPDRVAYLSALEG
                     AAQIASDGRLLQSARLNWPVSTGTRLILDPGTRAELHGGWSAVRLAERADLSVTRLDD
                     DAVQLALTAGSLSLRVRQLQPGERIEIDTPQLALVADQPGEYRLDVDPHGDTTRLTVH
                     AGSATVYGGAGQSSRVGPHQQVSYRGRDLLVTQAAPALRDALDQWAATRDALEDRSRS
                     AQHLPRELPGYAQLDAWGEWAQDATYGAVWYPSVTAADWAPYRHGRWAWIEPWGWTWV
                     DDAPWGFAPSHYGRWTQIGHRWAWVPGRFGPRPVYAPALVAFVGGASGSLGWGVSLGS
                     GAPAAAWFPLAPGEPWTPHYHASDRYRRRLNWDDGRERVRPPPDSFYFQRRPGAISVA
                     PVDHFGGGGDRGRRPHFGDGSRLPPGVLNESRVVAPPPRAYVPGMATQAPLPRPGGWP
                     TAPAPRPPQAVGPGHDVAPPPGAARPPRPGDGVQRPERPLPPRTPDLPGSDRRERDRW
                     LRDPDTRGQLPMPVPPNDAAARQQQLQRQLQQRQEALVRERQQREAQLAP"
     gene            complement(11140..11595)
                     /locus_tag="PKN51_10170"
     CDS             complement(11140..11595)
                     /locus_tag="PKN51_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNT85507.1"
                     /translation="MTLKDQITEDMKTAMRAKDSARVGTIRLLQAALKQKEVDERVTL
                     DDAAVIAIVDKLIKQRRDSIAAFTQAGRQDLADKETAEMQVLQAYLPARLSANDIAAE
                     VRAIVAELGAQGPGDMGKVMGAVKARLAGKADMAAASAAVKAALSGANS"
     gene            complement(11674..>11848)
                     /gene="rpsU"
                     /locus_tag="PKN51_10175"
     CDS             complement(11674..>11848)
                     /gene="rpsU"
                     /locus_tag="PKN51_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNT85508.1"
                     /translation="VALRRFKRTIEKLGLLTELRAREFYEKPTAERKRKKAAAVKRHH
                     KRVRSMQLPKKLY"
BASE COUNT         1671 a         3928 c         4304 g         1745 t
ORIGIN      
        1 tggagtcgct ggaagaaata gggcgacagg gcctgtacat gttcgacgat cctgcgttcc
       61 tggtagagcc tgatggtttc gagcgccacc gcagcggcca ccgggtgggc cgcgtaggtc
      121 aggccgtggg caaaggcgcc cagcttgcgg ctgccggcct tcagggcttc gtggatctgg
      181 tcggtgatca gcaggccgga gatcggctgg taggcgcccg acaggccctt ggccacggtc
      241 atcatgtccg gcttgagctg gaagctgtcg ctgccgaaca tctggcccgt gcgaccgaaa
      301 ccgcagatca cctcgtcgac gatgaacagg atgtcgtatt tcttcagaac ggcttgcacg
      361 cgatcgaaat agccggccgg cggcacgatc acgccgccgg aaccgttgat gggctcggcg
      421 atgaaagccg ccaccgtgtc cgggccttcc ttgaggatca actgctccag gttgttggcc
      481 aggcgttcgg caaactgcgc ttcggtttcg ctcggcagcg cgaagtggta gtggctgggg
      541 cagtcggtgt gcagcacctg cgggattggc aggtcgaaat cgcggtgcac gtgcaccaga
      601 ccggtcaggc tgcccgaggc cacggtgacg ccgtggtagc cgttgatgcg cgagatgatc
      661 tttttcttgc tgggtcggcc gagctggttg ttgtagtacc agaccagttt gaccgctgcg
      721 tcgttggctt cggagcccga gttggcgaac agcacatggt tgagccccgc cggcgcgatg
      781 gcggtgagcg cctcggccag ggcgatgccg ggcgggtgtg agcgatggtt gaacaggtgg
      841 tagtagggca gggtgcgaaa ctgcgccatc gcggcgtcaa tcaggcgctg ttcggaaaac
      901 cccagtgaag cgcaccagag gccggacatg ccttccaggt aacgcttgcc gttttcatcc
      961 tcgacatagc agccgtcacc gcgcgtgatg accacggggc cgcgccggtc gtgggcgtcg
     1021 aggttggagt aaggatgcac gacgtgatcg atatcctttt gccagttggg gctgggggtg
     1081 ctgctcatga ggtggccttg acgttggggt tcaatcggat gccaggtccc gaagcgagcg
     1141 gcttacgcgc gaagctgtgg ctcgtgggag cggacttgca gcgtaccccg cgagcgccgg
     1201 caggacttga ccgatcacga caatcgatcc ccttgcggcg gtcgatgctg ctcagtgggc
     1261 cgcggcctgg cggtacgcgc gtgggctgac gcgcgcatgg ctgcggaagg cgcgcgtgaa
     1321 ggcgctggtt tcgctgaagc ccagttgttc ggcaatgcag gtgacgggca gccggctttc
     1381 cgtcagcagg cggcacgcgg tctgcatgcg cacctgcgct agcaggtcct gaaaaccgac
     1441 gccctgttcc ttcagccgcc gctgcagcgt gcgcggcgtg acgccgaagt cgctggcggc
     1501 gtgctccagc gtggcccgcc cctcgctggc ggcatagctc atccaggtcc gcaaggcgtc
     1561 gcccagcagg cccggggtgg cctggttcgg catgcgcagc tccagttggg cgcagaagtt
     1621 gcggaatcgt tcgaggtcgg cggtcgggat ggggcactcc agcacctcgg ccgcgaaatg
     1681 caggccggtg tatgccgcac tgcaaaccac gggcgcgccg aagaagcgct gataggcgtc
     1741 ggcgccgccg ggggccggca tcgccagcgt gacgtgatcg agccgccagg atggnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctgggg cggctcggca tcgaaggcgg
     1921 ccatttccag gcccagttgc tggcggccac tttgctgcgc ggcgtggctc agcaggcgcg
     1981 tgaagcccag caagggctgc gggtcatcct ggcgctcgcg ttcggcgcct ggcgcccagg
     2041 gccagggcct cgcgggcagg ggcaccgggc ggctcaacag gcggtgcagg acgtgcgtgg
     2101 ccaggcgagt ggcctcaccg ctgatggcgc caaccagaag tcttgtggtc atgggcgggc
     2161 gtccgacctg ggaagtgcat tgaattccgt aaaatggaaa gtgaatttta atatagcaaa
     2221 ttaaccaaaa ccattagggt gaaccccggc gtgcagcggg tggcttgggc gctgtggatc
     2281 gctcgggcgg gttcatgggg cgtgccggca gcgggtcgat ggccgcagct tgtgcctgcc
     2341 ggggggcgcc gtcaagtggc aacgcgccgc cgcccggcat gcgcggggtg ggcggggggc
     2401 tgcgcggtgc ccgcagcgcc cgatcgcagg cgctggcgtg cgatcagggt gtggccgcca
     2461 gccgcaggcc gggcccgcgc gtgcgccaca gcagccagct ggcaatggcc aggttcagca
     2521 ggttccagcc gatgccgttg aggaaggcgg cgcggtagct gccggtcagg tcgaacaccc
     2581 agccgctcag ccagcctccc agcgccatgc ccagcagcgt ggccatgatg accgcgccgg
     2641 tgcgggcgcc ggcttcgcgt gccggaaaat gctcgcgcac gatgatggcg taggacggca
     2701 cgatgccgcc ctgaaacagc ccgaacaggg ccgacaccac gtacagcggc accagcccgt
     2761 cgaccggcag gaacagcagc agcgccacgc cctgcagggc cgaacccagc agcagcgtgc
     2821 ggatgccgcc gatgtggtcg cagatccagc ccgacaccag tcggctgacg atgccgctgg
     2881 cgagcatcag cgacagcatc tcggcgccgc gcgccgcgcc aaaacccagg tcactgcagt
     2941 aggccacgat gtgcacctgc ggcatcgaca tggccacgca gcaggccacg ccggccacgc
     3001 acagcagcca ttgggcctgt gccggcgtca ggccgaacgg gcgttcgggg ggccagggcg
     3061 tggcgcgtgc cgcggcggcg ccgtggctgg ccggatgggc cgacagcgcc ggcgggcgcc
     3121 gccgcatggc cagcgccagc agcgccatgc cgacgccaca caccaggccc agcgccacgt
     3181 aggtgggccg ccagccgtag tgggtgatgc cccattgcgc cagcggcggc cacagcgtgc
     3241 cggcgatgta attgccactg gcgcagatgg ccatggcgat gccgcgtcgc cggtgccacc
     3301 acaggccggt gtcggccatc agcggcgcga aggtggcggc cccgcccagc aggcccagca
     3361 ggccgtgcgc cagaccgaac agccacacgt tgcccgacag cgccgccagc aggtaaccgg
     3421 cgccgaccgc cgccgcgccc agccacagca cgcgcgcgat gccccagcgg tcggcccaca
     3481 ggccggtcca caagccgccc agccccaggc agaccatcat cagggtgtag ggcagcgagg
     3541 cgccggcgcg gccgatgccg aattcggcct gcaccgccgg cagcaccacc gacaccacgt
     3601 acatggcgct gttgcccagc gtcaccagca acaccaccac cagcaggcgc caggcggcgg
     3661 tgcgcgagtc gatcaggctg gcgtcggacg gcgtggcggc ggcggacatg gttgcctgcg
     3721 cggtcagccg aagcggcaga tgtagtgata gacctcggca acgcggatct cgaagctgga
     3781 gttggccggg atgctgaagc actcgcccgg cccggacgac ttccaggacg tttcgccggc
     3841 cagacggtat tcgcagccgc cggccacgca ttccatgact tcggccgcgc cggtgttgaa
     3901 gctgagggtg gcgggcagga tcacgcccac gctggcccgg ctgccgtcgg cctgctgaaa
     3961 gccgtgcgag acgcacttgc cgtcgaagta gacgttggcc tgggtggtga gggtgacgtt
     4021 ggcgatgtgc gaggtggtca tggatcggtg acagggttgc aggaacaagc cgcgcatgtt
     4081 atcgaggcgg tgacaatcct gcccatgaac cccccgcgca cccgcttcgt gctgatcgaa
     4141 accagccacg ccggcaacgt gggcgcggtg gcgcgcgcca tgaaggtgat gggtttcgac
     4201 gacctggtgc tggtgcgacc ccgctggccc gatgtgctgt cgcgtcccga ggcgatcgag
     4261 cgcgccagcg gcgccggcga cgtgctggcg cgcgcgcgct gtgtggccac gctggacgag
     4321 gcgctcgacg gcatgacgca cctgtgcgcc acggcgatga cgccgcgcga cttcgggccg
     4381 cccacgcggg cgccgcgcgc gcacttcgat ttgctattga aacaggagct gtcggcgcac
     4441 gtcccgcaag cgccagcgct cgattttgat cttgaagcgg ggcagggcgt ggcgctgctg
     4501 ttcggctccg aacgctacgg catgcgcaac gaggacgtgt accgctgtca tgtcgcgctc
     4561 agcatcccga ccgatccggc ctacggctcg ctcaacctcg gcgccgccgt gcagctgatc
     4621 gcctacgact ggcgtcaggc gctggcggcg cgcttcggcg gcttcgacat cgcgccggcc
     4681 ggcgccgcgc cgcgccacgc cgacgcggcg cagctgggcg ccatgctggc gcactgggag
     4741 caggcgctgg tggccatcgg ctttctggat ccggccgcgc ccaagaagct gatgccgcgc
     4801 ctgcagcagc tgttcaaccg cgcccagccg acgctggaag aggtgcacat cctgcgcggc
     4861 atcgcccgcg ccatgcagcg ggcggcgtcg tccggccagc aggacgatgg cgcgccggcg
     4921 ccgcgtccgc gctagacttc gcaccccgat gttgtcccgc atccgttcct acacccaggc
     4981 catcctggag cgcgatcccg ccgcccgcag cgtgtgggac gtggtgtttt cgtacccggg
     5041 cttccatgcc ctggtgctgc accggctggc gcacccgctg tggaacgccg gctggcactg
     5101 gctggcgcgc tggatctcgc actgggggcg tttcctgacc ggcatcgaga tccacccggg
     5161 cgccaagatc ggcgagcgtg tgttcatcga tcacggcatg ggtgtggtga ttggcgagac
     5221 cgccgagatc ggcgacgact gcaccatcta ccaaggcgtg acgctgggcg gcacctcgct
     5281 caccaagggc agcaagcgcc acccgacgct gggcaagggc gtggtggtgg gggcgtccag
     5341 ccagattctg ggcggcttca ccgtgggcga cggcgccaag atcggctcca gcgcagtggt
     5401 caccaagccg gtgccggcgg gcgccacggc ggtgggcaac ccggcgcgca tcatcctggc
     5461 cgacgaccag acgcggcgcg aggaagccgc ggccaagctg ggcttctcgg cctacggcgt
     5521 gacgcagagc gacgacccgg tgagccaggc gctgcgcggt ctgatcgacg gcaccgccac
     5581 gcacgagcac cagatcgcct tgctgtggca ggcggtggaa aagctggcct gccagatcga
     5641 cccggactgc gtgccgggcg atgcctcgcg gcaggaaacc ttcgaggccg acaagctcaa
     5701 ccagctggtc ggcaagtgac ggtgcgccgc aggcgcgggc ctcagcgccg gcgctggttc
     5761 ttgccgactt cgtgaccgat cagggcgccg gcggcggcgc caccgatggt gcccacaggg
     5821 ccgccgaaca gcacatggcc ggtggcaccg cctaccaccg caccgacgcc ggtgccgatc
     5881 tgggcgttgg tcgggtggct ggcgcagccg gcgctggtca ggccggccag cagggcggcg
     5941 gcgagcgcca ggcggcgcca gggggacgat ggggtctgca tgggggtgaa gcgtgtcatg
     6001 gcgggcatga tcgcccgcat cccccgttgg ggtgtgacaa acccggcgca cttgcaacca
     6061 gaggtcgcac ggcgtgggtg gcgcggttac gatgggagac cgattttgcg gtgtttccgg
     6121 gctccagcgc ttgactgacc tgcgccagca gctcccgatt caatagcaca tatggtattg
     6181 ggccagcgcc cggcccggcg tggacggcag cggcctcagg cccggaccgg gcgctgcgcg
     6241 ttcttgggcc ggaaggcctt acagacggtc tcgtcggttt ccaggtaggg gccgccgatc
     6301 aggtcgatgc agtagggcac ggcggcaaag atgccatgca cctgcggccg gccgtcggcg
     6361 tccttcagcc cttccagcgt ctcggcgatg gccttgggct gcccgggcag gttgatgatc
     6421 aggctctggc cgcggatcac cgccacctga cggctcagga tggcggtcgg cacgaaggcc
     6481 aggctgatct ggcgcatctg ctcgccaaag ccgggcatga gcttttcggc caccgccagc
     6541 gtggcctcgg gcgtgacgtc gcgcggcgcc gggccggtgc cgccggtggt cagcaccagg
     6601 gcgcagccgg cgtccaccag ctcgatcagg gtgcggctga tttccacctg ctcgtcgggg
     6661 atcaggcgcg gttggaaacg gatcggattc ttcagcgccc ggcccagcca ttcctgcagc
     6721 gcgggcagac ccttgtcttc ataggcgccg ctggaggcgc ggtcgctgat ggagacgatg
     6781 ccgatggtga cggggtcgca ggtgttcatc gaagttgcta tgtgaatggt agctggtggc
     6841 gctcagccgg cgcgggtgtc gtcgctgtag ggcacttcat cgtcagggtt ggggccggcc
     6901 gcttcggcac gcgccagcgc cgcctggatg agctggaaca gctcgcgcca ggcacgtccg
     6961 tggcgcgctg cctggccgcg ggcgcgcgcc gcggcggcgt cggcgggcgc ggcggcgtcc
     7021 ttgcgcgcct gacgcaccag cgcgcgcagc tgctgcgcat cgctgtccgg aaactggccg
     7081 atccaggcgc tcagcgcggc gtcgtcggcg accaggcgct cgcgccagcg ctcggcggcg
     7141 tgcaggcgca gggtgtcgcg cgcggcgccc tggcgctgtt cgtgcagcgc ggcgcggatg
     7201 gcggccacgt cttcgcccgg cagctggcgc atcagcttgc cgacgtactg gctctggcgc
     7261 cgcttgccct cgaagttggt gatgcgctgc agctcctgca gcgcctccag cagccgcgcc
     7321 ggcagcgcca gcggctgcag caggtcggcg cgcaggctca gcaactgctc gcccagggcc
     7381 tgcaactcgg cgctctgggc cttcagctcg gtcttgctgg ggccgtcggc gagctgctcg
     7441 cgccgcagtt cttcgtccag ctcgctgcct tcggccacga actggccgcg cacgtaatag
     7501 ccttttctgg gtttgcgaga catgggtgaa gcggtcaaaa gtcggccagt atgatagccg
     7561 ccaccatgca agacacctcc cagccccagg cgggcttttc ctattcgcgc gcccggttcg
     7621 aggagctggt ggacacggcc atagcccacg ccagggcgct gggcgccacc gacgccggtg
     7681 ccgaagcgtc cgagggcgtg ggcctgagtg tgggcgtgcg catgggcgag ctcgagacgg
     7741 tcgagcgcaa ccgcgacaag tcgctgggcg tgacggtcta cctgggccag cggcgcggca
     7801 acgccagcac ctcggatttt tcacgcgccg ccatcgagcg caccgtgcag gcggcgtacg
     7861 acatcgcgcg gttcaccgcc gaagacccgg ccgccggcct gcccgatccg gccgatgtgg
     7921 cccagccgca cgagcagcgc gatctggacc tgtttcatcc ctggagcatc gacagcgaac
     7981 aggcggcggc gctggcgctg gcctgcgagc gcgccgccct ggcggtggac cggcgcatca
     8041 ccaacagcga aggcgccagc gtgtcggcgc agcaaagcca cttcttcagc gcccacacgc
     8101 atggctttcg cggcggctac gccagctcgc gccacagcct ttcggtggcg ccgatcgccg
     8161 gcaggggcga ggacatgcag cgcgactact ggtacagctc gatgcgcgac gccgccgagc
     8221 tggccgcgcc cgaggccgtg ggccgctacg ccgccgagcg ggcgctgagc cgcctgggca
     8281 gccgcaagat cgccaccggc gaatgtccgg tgctgttcga gtcgccgctg gcggccggcc
     8341 tgctgggcag cttcgtgcag gcggtcagtg gcggcgcgct gtaccggcgc acctcgttcc
     8401 tgctggattc gctgggcaag ccggtgtttg ccgaccacat cgacgttggc gaagacccct
     8461 tcgtgcgcag cggcaagggc tcctcgccgt tcgacgagga aggcgtgcgc gtgcagccgc
     8521 gccaggtggt cgaagcgggc cgcctgcagg gctactttct cagcacctat tcggcgcgca
     8581 agctgggcct gaagaccacc ggcaacgccg gcggctcgca caacctgacg ctgagctcgc
     8641 gccgcacccg ggccggcgac gatctggacg ccatgctgcg ccagctcggc accggcctgt
     8701 tcgtcaccga gctgatgggc cagggcgtga actacgtcac cggcgactat tcgcgtggcg
     8761 ccagcggctt ctgggtcgaa aacggccgca tcgcctaccc ggtgcaggaa atcaccatcg
     8821 ccggcaacct gcgcgacatg tttaggggca tccaggccgt cggggccgac acctacacct
     8881 acggtgccaa gaccgtcggg tcgatcctga tcgaccgcat gacggtggcg ggcaactgat
     8941 cctgtggctt tgaaccgttc aaccagcgct tgacccatgc gcctgccgtc atgcaggcgc
     9001 agcgcggtca tgggtcatcc gtcatggggc gcaacgcgtg ttcgcgctgc gccgctcagc
     9061 cgcccatgcc ttccgcagaa cgcagggtgg gcgcccgccc ggggcgttcg cggggctcgg
     9121 actcgcgctc acggcgctgc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnggcgccag ctgcgcttca cgctgctgac gctcgcgcac cagggcttcc tggcgctgct
     9301 gcaactgccg ctgcagctgc tgttggcgcg ccgcggcatc gttcggcggc accggcatcg
     9361 gcagctggcc acgggtgtcg ggatcgcgca gccagcggtc gcgctcgcgg cggtcgctgc
     9421 ccggcagatc gggcgtgcgc ggcggcagcg gccgttcggg ccgctggacg ccatcgccgg
     9481 ggcggggcgg gcgtgcggcc ccgggtggcg gcgccacgtc gtgacccggg ccgacggcct
     9541 gcggggggcg gggggcgggt gcggtgggcc acccgccggg gcgcggcagc ggcgcctgcg
     9601 tggccatgcc cgggacgtag gcgcgcggcg gcggcgccac cacacggctt tcgttcagca
     9661 cgcccggggg caggcggctg ccgtcgccga aatggggccg gcgaccgcga tctccgccac
     9721 cgccgaaatg gtcgaccggc gccacgctga tggcgcccgg ccggcgctgg aagtagaagc
     9781 tgtccggcgg cggccgcacg cgttcgcgcc cgtcgtccca gttgaggcgg cggcggtagc
     9841 ggtcgctggc gtggtagtgc ggcgtccagg gctcgcccgg ggcgagcggg aaccaggccg
     9901 ccgccggtgc gccgctgccc agcgatacac cccagcccag gctgccgctg gcgccaccga
     9961 cgaaggccac cagcgccggc gcgtataccg ggcgcggccc gaagcggccg ggcacccagg
    10021 cccagcggtg gccgatctgg gtccagcggc cgtagtgcga gggggcaaaa ccccagggcg
    10081 cgtcgtccac ccaggtccag ccccagggct cgatccaggc ccagcggccg tggcggtagg
    10141 gcgcccagtc ggccgcggtg acgctggggt accagacggc gccgtaggtg gcgtcctgcg
    10201 cccattcacc ccaggcgtcg agctgggcgt agccgggcag ctcgcgcggc aggtgctggg
    10261 ccgagcgcga acggtcttcc agcgcgtcgc gcgtggcggc ccactggtcg agcgcatcgc
    10321 gcagcgccgg cgccgcctgg gtgacgagca gatcgcgccc gcggtagctc acctgctggt
    10381 gcgggccgac ccgggacgac tgtccggcgc cgccgtacac ggtggcgctg ccggcatgca
    10441 ccgtcaggcg cgtggtgtcg ccatgcgggt cgacgtccag ccggtattcg cccggctggt
    10501 cggccaccag cgccagttgc ggggtgtcga tctcgatgcg ctcacccggc tgcagctggc
    10561 gcacacgcag cgacaggctg ccggcggtga gcgccagctg gacggcgtcg tcgtcgagcc
    10621 gggtgacgct caggtcggca cgctcggcca gacgcacggc gctccagccg ccgtgcagct
    10681 cggcgcgcgt gccggggtcg aggatgagac gggtgccggt gctcaccggc cagttcaggc
    10741 gggcgctctg cagcaggcgg ccgtcgctgg cgatctgcgc cgcgccttcc agtgcgctga
    10801 ggtaggccac gcggtccggc gggtcggcct gcgcccagcc gagcagcggt gccagcagca
    10861 gcaacgcgcc ggcgcaccag cggcgccagc cggagtgaag ggggttcagt gtcatggcct
    10921 gcatcgggcc ggcgcaggaa gcctgggctg caccggctcc gattcaacgc gccggcatgg
    10981 cgggcggttt gccgcgccat gtgtcgggcg tgcaaagtcg ggggtgccgt ttgcgtccaa
    11041 atgtgacgac cggcccagcc ggggggcgct gcccggccct tggtttgtga gacggatcgg
    11101 ccgctggcgc ttgccgggag agcgccagca gctattgttt caggagtttg cgccgctcag
    11161 ggcggctttg accgcggcgc tcgccgcagc catgtcggcc ttgcccgcca gccgcgcctt
    11221 gacggcgccc atgaccttgc ccatgtcgcc cgggccttgg gcgcccagtt cggccacgat
    11281 ggcgcgcacc tcggcggcga tgtcgttggc cgacaggcgc gccggcaggt aggcctgcag
    11341 cacctgcatt tccgcggttt ccttgtcggc caggtcctgc cggccggcct gggtgaaggc
    11401 ggcgatgctg tccctgcgct gcttgatcag cttgtcgacg atggcgatga cggcggcgtc
    11461 gtccagggtc acgcgctcat cgacctcctt ctgcttgagc gccgcctgca gcaggcggat
    11521 ggtgcccacc cgcgcactgt ccttggcgcg catggcggtc ttcatgtctt cggtgatctg
    11581 gtccttgagg gtcatggcgg tggtccggac gaaaagcaaa aaggcccgct gagcgtcccc
    11641 ggcgagcctt tgaagggtgg cgcaagaagc cgatcagtac agcttcttgg gcagctgcat
    11701 gctgcgcacg cgcttgtggt ggcgcttgac ggcggcggcc ttcttgcgct tgcgctcggc
    11761 ggtgggcttt tcgtagaact cgcgggcgcg caactcggtc agcaggccca gcttttcgat
    11821 ggtgcgcttg aagcggcgca gggcgacg
//
LOCUS       DAOQTZ010000085        11327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_6458_length_11327_cov_14.494766, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000085 DAOQTZ010000000
VERSION     DAOQTZ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11327
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6458_length_11327_cov_14.494766"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKN51_10180"
     CDS             complement(<1..593)
                     /locus_tag="PKN51_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNT85509.1"
                     /translation="MRLFTSPRAPNPRRVLMFMHEKGIAGVEQVTVDLNAGEHQSAAY
                     RAKVPVARVPALELPDGRVLSETRAICSYLEGLHPEPNLMGEGFDERAFIEMHDRRVE
                     WYWLLPIANCVRHTHPGLAALEQPQFPEFGRSQGQKVRESARWLDEALARQPWVAGER
                     FTIADITAFCALEFARLMKFSAGAEGFAALQAWRDRVA"
     gene            complement(681..1169)
                     /locus_tag="PKN51_10185"
     CDS             complement(681..1169)
                     /locus_tag="PKN51_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNT85510.1"
                     /translation="MTSRRWKPAVTVAAVIEQQGRYLLVEERTAAGLQLNTPAGHLEP
                     GESPQAGAVREALEETGRLFAPSALLGVYLAASTDAQGQPATWLRLAFCGTAGEPDPA
                     RRLDQGIVRTLWLTPAEIEASRSRHRSPLVWRCVQDHLRGQRFDLSAVVMDPSALGEP
                     PC"
     gene            1342..2502
                     /locus_tag="PKN51_10190"
     CDS             1342..2502
                     /locus_tag="PKN51_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNT85511.1"
                     /translation="MLIGVPAETAAGETRVAVTPETAKKLVAQGHVVRVQAGAGLRAA
                     AIDEAYRTAGSEITDAAGALGAEMVLKVRSPAPAELAQIQPGSVLVGMLEPFNAEGLQ
                     RLAAASLTAFALEAAPRTTRAQSMDVLSSQANIAGYKAVIMAADRYQKFFPMLMTAAG
                     TVKAARVVILGVGVAGLQAIATAKRLGAVIEASDVRPSVKEQVESLGAKFIDVPYETA
                     EEKEAAEGVGGYARPMPQSWLERQKVEVAKRVAQADVVISTALIPGRAAPVLITEEMV
                     QSMKPGSVIIDMAAGKGAPNADGTVGGNCPLTEADRTVVKHGVTIVGETNLAAQVAAD
                     ASALYARNVLDFLKLVLPPAAKGEPPATFRLDLEDDIVAACLMAHQGAVTRK"
     gene            2499..3320
                     /locus_tag="PKN51_10195"
     CDS             2499..3320
                     /locus_tag="PKN51_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85512.1"
                     /translation="MSAARLTRSRGMLALAASALLAACAAPGGETSATGVAAPTASVA
                     APHIQPPPTSVGTWVELGHVQAPWLAGDAPVPVSGPVAPTRVAGLRRDDGRWLAIVMV
                     QRATGTAVPCPRPTGVSVTTEGDGCLRLRRDADFDRWLERQHGVLHQWVESRGWAARP
                     RAWVAYRLPHTGSQALEAHVLLDPALIEPTTRGSADFLAAGQPGLDWARALAAATRTA
                     DGSGVLALPALPFAPPVTPVAPPVATPAVVTAPPAARTPPPPPRPAATAPRRDRG"
     gene            3373..3690
                     /locus_tag="PKN51_10200"
     CDS             3373..3690
                     /locus_tag="PKN51_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNT85513.1"
                     /translation="MDAVSPTLINLIIFVLAIYVGYHVVWTVTPALHTPLMAVTNAIS
                     AIVIVGAMLAAALTEGSFAKLLGVAAVALAAVNIFGGFLVTRRMLEMFKKKDRPAKAE
                     AAK"
     gene            3703..5136
                     /locus_tag="PKN51_10205"
     CDS             3703..5136
                     /locus_tag="PKN51_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNT85514.1"
                     /translation="MSMNLVTLLYLIASVCFIQSLKGLSHPTTSIRGNLFGMVGMTIA
                     VLTTAALIMRLAGSALGLGWVLLGLVLGGGYGAWRAKTVEMTKMPELVAFFHSMIGLA
                     AVFIAGATVIEPWAFGLAKSVAQGGDGQIPGGNRIELALGAFIGAVTFSGSVIAWGKL
                     SGKSKFRLFQGAPVQFAGQHWVNLGLGLLTAFFIFGFWHSQSHLDFALVCLLGFIIGV
                     TLIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCK
                     AMNRSFFSVILGGFGGEAVTAGGAQEQRPVKSGSADDAAFMLGNADSVIIVPGYGLAV
                     ARAQHAVKELVDKLTDKGIDVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVHEMEDVNG
                     EFGQADVAIILGANDVVNPAALQKGSPIYGMPILEAYKAKTVIVNKRSMAAGYAGLDN
                     ELFYMDKTMMVFGDAKKVVEDIGKAIE"
     gene            5294..7000
                     /locus_tag="PKN51_10210"
     CDS             5294..7000
                     /locus_tag="PKN51_10210"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HNT85515.1"
                     /translation="MTERSWLKHYPAGVPADIDTTQYSSLVALIEESFSKYADRTAYS
                     FMGKDVSYADTDADSRALAAYLQSLGLQRGDRVALMMPNVPQYPASVAAVLRAGFVVV
                     NVNPLYTARELEHQLKDAGAKAIIILENFAATLQQCIARTQVKHVVLASMGDRLGGLK
                     GMIVNLVVRKVKKMVPPFSLPGAVRFNDALARGRSAPFAAPPIGPDDLAVLQYTGGTT
                     GVAKGAMLLHRNLIANVLQSEAWNDPVMKSIPAGEQPTSVCALPLYHIFAFTVNMMLS
                     MRTGGKTILIPNPRDLPAVLKELSKHTFHSFPAVNTLFNGLANHPDFNTVNWKNLRVS
                     VGGGMAVQSAVAKLWLEKTGCPIVEGYGLSETSPSASCNPVTSKEFSGTIGLPLPSTY
                     LKILDDSGAEVPLGQPGEIAIKGPQVMAGYWQRPDETAKVMTPDGYFKSGDIGIMDER
                     GYTRIVDRKKDMILVSGFNVYPNEIEDVVAAIPGVLEVGAVGVPDEKMGEAIKLVIVK
                     KDPGLTEEAVRAYCKQNLTGYKQPRVIEFRAELPKTPVGKVLRRELRDPAPAKAAAAA
                     VD"
     gene            7025..7813
                     /locus_tag="PKN51_10215"
     CDS             7025..7813
                     /locus_tag="PKN51_10215"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNT85516.1"
                     /translation="MALPPGASDGGRPRRTGVVAAMHPELAAVLERLPDEQRQRVAGR
                     DFWVGHWHAHEVVAVLSGVGKVAAATTATLLIERFGVDALLFTGTAGGLGDGVRVGDL
                     VLAGEFLQHDMDASPLFPRFEVPLYGRARFATDAALSARLGAAARQVLARASDTLGAH
                     AVAEFALHAPAVHRGLVVSGDRFVATSAESAALRQALPEALAVEMEGAAVAQVCHDYG
                     LPFAALRTVSDRADDSAHVDFMRFIDQVASRYSAAVLEAFFAEL"
     gene            complement(7879..8865)
                     /gene="corA"
                     /locus_tag="PKN51_10220"
     CDS             complement(7879..8865)
                     /gene="corA"
                     /locus_tag="PKN51_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNT85517.1"
                     /translation="MLNVFTLSNGRLFQEEIEALESLSRYRPIWVDLEDPTPEEKRWV
                     KQYYGLSIPEDAIDDDLEESARFYEEDNGELHIRSDFLLADSDTPRQLRVAFILNQSN
                     DQLRSHGVLFSIHDESVPAFRLLRMRARRMPGLLEDAKDVLLALFDGDAEYSADALEE
                     IYDELEAAGKMVLDESVTDEVASEVLATIARQEDMNGRIRRNVMDTRRAVSFLMRSRM
                     LDAEQFEEARQIQRDLDSLDGHTTFLFDKINFLMDATVGFININQNKIIKLFSVASVA
                     LLPPTLVASIYGMNFKAMPELDWQLGYPYAIALMIASALVPMWYFRRRGWLK"
     gene            9209..9541
                     /gene="raiA"
                     /locus_tag="PKN51_10225"
     CDS             9209..9541
                     /gene="raiA"
                     /locus_tag="PKN51_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNT85518.1"
                     /translation="MNMTISGHHLEITPALRSYVSTKLDRVIRHFDQVVDVKVLLSVE
                     NQKEKDKRQRAECRIGVKGNDLFAESSHEDLYAAVDELVDKLDRQVARYKEKQQSHDK
                     NTAVKHLM"
     gene            9780..10256
                     /locus_tag="PKN51_10230"
     CDS             9780..10256
                     /locus_tag="PKN51_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNT85519.1"
                     /translation="MNRLASILPEAQVLVGVEATSKKRAFEEAGLLFESLHGLSRALI
                     TDSLFARERLGSTGLGHGVAIPHGRIKGLKAPMAAVLRLAQPIGFDAPDEQPVSLLIF
                     LLVPEAATQKHLEILSEIAELLSDASLRERIKGATSAPELHGLIAHWQSGQPVAQA"
     gene            10309..11265
                     /gene="hprK"
                     /locus_tag="PKN51_10235"
     CDS             10309..11265
                     /gene="hprK"
                     /locus_tag="PKN51_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNT85520.1"
                     /translation="MKPSAISADVLFEDHRAVLRWQWMAGLEASERRFDEIAVRAARS
                     GADLVGYLNYIHPYRVQVLGEREVAYLTNASSEDCARRIARIVTLEPPVLVLCDGQNP
                     PEALTALCERAHIPLFATQESAAFVVDVLRAYLSRHFADRTTMHGVFMDILGLGVLIT
                     GESGLGKSELGLELISRGNGLVADDAVDLFRINQTTIEGRCPELLRNLLEVRGIGLLD
                     IRAIFGETAVRRHMRLRLIVHLVRRETLERDYERLPYEPLTQEVLGVPIQKVVIQVVA
                     GRNIAVLVEAAVRNHILQLRGIDTYQEFVERHRRAMEQGSSA"
BASE COUNT         1834 a         3957 c         3837 g         1699 t
ORIGIN      
        1 gccacccggt cgcgccaggc ctgcagggcg gcaaagcctt cggcgccggc gctgaacttc
       61 atcagcctgg cgaattcgag ggcgcagaag gccgtgatgt cggcgatggt gaaacgctcg
      121 ccggccaccc agggctggcg cgccagcgcc tcgtccagcc agcgggcact ctcacgcact
      181 ttctgaccct gcgaccggcc gaactcgggg aactgcggct gctccagcgc cgccaggccg
      241 gggtgggtgt ggcgcacgca gttggcgatc ggcagcagcc agtaccactc cacccgccgg
      301 tcgtgcattt cgatgaaggc gcgctcgtcg aagccctcgc ccatcaggtt cggctcgggg
      361 tgcaaacctt ccagatagct gcagatggcg cgcgtttcgc tcagcacgcg accgtcgggc
      421 agctccagcg ccggcacgcg cgccaccggc accttggcgc ggtaggccgc ggactggtgt
      481 tcgcccgcgt tcaggtcgac cgtcacctgt tccacgccgg cgatgccctt ttcgtgcatg
      541 aacatcagca cgcggcgcgg gttgggggcg cggggggaag tgaacagtcg catggcttgc
      601 tcctataaat atagctgctt gcgcttacca ggcaagcgcc agagcccaaa aatcattgtc
      661 aaccacgggc ggcggcacct tcagcacggc ggctcgccaa gcgccgacgg gtccatcacc
      721 accgccgaca gatcgaagcg ctggccgcgc aggtggtcct gcacgcagcg ccacaccagc
      781 gggctgcggt gccgtgaccg gctggcttcg atttcggccg gggtcaacca cagggtgcgc
      841 acgatgccct ggtccagccg gcgcgccggg tcgggctcgc ccgccgtgcc acagaaggcc
      901 aggcgcagcc aggtggcggg ctggccctgg gcgtcggtcg aggccgccag gtacacgccc
      961 agcagggcgc tgggcgcgaa caggcggccg gtttcctcca gcgcctcgcg cacggccccg
     1021 gcctggggcg actcgcccgg ctccaggtgg ccggccgggg tgttcagttg cagaccggcg
     1081 gcggtgcgct cttccaccag caggtagcgg ccctgctgct cgatcacggc agccacggtg
     1141 accgcgggtt tccatcggcg tgacgtcatg ccagccatta tcggcagcgc cgcgccgcgg
     1201 tcgccgtcgc ggcaggcggc gcaccaaaat gacgcacaat cgacgccaag agcgcgcagc
     1261 cgcgcactcg atcaccgaca aacccgccca gccggcccgc gcccgagcgg accgatggct
     1321 ggctcacgag aggagtgttt catgctgatt ggcgtacccg ccgagacggc ggccggcgaa
     1381 acccgggtgg ccgtgacccc ggaaacggcc aagaagctgg tggcccaggg ccatgtcgtg
     1441 cgtgtgcaag caggcgccgg cctgcgcgcg gccgccatcg acgaggccta ccgcaccgcc
     1501 ggctccgaaa tcaccgatgc cgccggcgcc ctgggcgccg agatggtgct gaaggtgcgc
     1561 agccccgcgc ccgcagagct ggcgcagatc caacccggca gcgtgctggt gggcatgctg
     1621 gagccgttca acgccgaagg cctgcagcgc ctggcggccg ccagcctgac cgcttttgcg
     1681 ctggaagcgg cgccgcgcac cacgcgcgca cagagcatgg acgtgctgtc cagccaggcc
     1741 aacatcgccg gctacaaggc cgtcatcatg gcggccgacc gctaccagaa gttcttcccc
     1801 atgctgatga ccgccgccgg caccgtcaag gccgctcgtg tggtcatcct gggcgtgggc
     1861 gtggccggcc tgcaggcgat tgccaccgcc aagcgcctgg gcgcggtgat cgaagccagc
     1921 gacgtgcgcc ccagcgtgaa ggaacaggtc gagtcgctgg gcgccaagtt catcgacgtg
     1981 ccctatgaaa ccgccgagga aaaggaagcc gccgagggcg tgggcggcta cgcccggccg
     2041 atgccgcaga gctggctgga gcgtcagaag gtcgaagtgg ccaagcgcgt ggcccaggcc
     2101 gacgtggtga tctccaccgc cctgatcccc ggccgcgccg cgccggtgct gatcaccgaa
     2161 gagatggtgc agagcatgaa accgggctcg gtcatcatcg acatggcggc cggcaagggc
     2221 gcacccaacg ccgacggcac ggtgggcggc aactgcccgc tgaccgaggc cgaccgcacc
     2281 gtggtcaagc acggcgtcac catcgtcggc gaaaccaacc tcgccgccca ggtggcggcc
     2341 gacgcgtcgg cgctgtacgc gcgcaacgtg ctggacttcc tcaaactggt gctgccgccg
     2401 gccgccaagg gcgagccgcc ggccaccttc aggctggacc tggaagacga catcgtcgcc
     2461 gcctgcctga tggcgcacca gggcgcggtg acccgcaaat gagcgccgca cgcctgacgc
     2521 gcagccgggg catgctggcg ctcgccgcca gcgccctgct ggccgcctgc gcggcgcccg
     2581 ggggcgagac ctccgccacc ggcgtggccg caccgacggc cagcgtggcg gcgccgcaca
     2641 tccaaccgcc gcccaccagc gtcggcacct gggtcgagct cggccacgtg caggccccct
     2701 ggctggccgg tgacgccccg gtgcccgtca gcggcccggt ggcgccgacc cgcgttgccg
     2761 gcctgcgccg ggacgatggt cgctggctgg ccatcgtgat ggtgcagcgc gccaccggca
     2821 ccgccgtgcc ctgcccacgc cccaccgggg tgtcggtcac caccgagggc gacggctgcc
     2881 tgcgcctgcg gcgcgacgcc gacttcgacc gctggctgga acgccagcat ggcgtgctgc
     2941 accagtgggt cgaaagccgc ggctgggcgg cacgcccgcg cgcctgggtc gcctaccggc
     3001 tgccgcacac cggcagccag gcgctggaag cccatgtgct gctggacccg gccctgatcg
     3061 aaccgaccac ccgcggcagc gccgactttc tggccgccgg ccagccgggc ctggactggg
     3121 cgcgcgcact ggccgccgcc acccggaccg ccgacggcag cggcgtgctg gcgctgccgg
     3181 ccctgccctt cgcgccgccg gtcacgcccg tcgcaccgcc ggtcgccacc ccggccgtcg
     3241 tgaccgcccc gccggcggca cgcaccccgc caccaccgcc gcgcccggcg gccaccgcgc
     3301 cgcgccgcga tcgcggctga gtcggccacc gcaccgacac gcacccgata ccaccatccc
     3361 gaggagccct gcatggacgc cgtatccccc accctcatca acctgatcat cttcgtgctg
     3421 gccatctacg tgggctacca cgtggtgtgg accgtcacgc ccgcgctgca cacgccgctg
     3481 atggcggtca ccaacgccat ttcggccatc gtcatcgtcg gcgccatgct ggcggcggcg
     3541 ctgaccgagg gcagctttgc caagctgctg ggcgtggccg cggtggcgct ggccgcggtg
     3601 aacatcttcg gcggctttct ggtgacgcgc cggatgctgg aaatgttcaa gaagaaagac
     3661 cgtcccgcca aggcggaagc ggccaagtaa ggagcgaccg acatgagcat gaacctcgtc
     3721 acgctgctgt acctgatcgc cagcgtctgc ttcatccagt ccctcaaggg gctgagccac
     3781 cccaccacct cgatccgggg caacctgttc ggcatggtcg gcatgaccat cgccgtgctg
     3841 accacagcgg cgctgatcat gcggctggcc ggctcggcgc tgggcctggg ctgggtgctg
     3901 ctgggcctgg tgctgggcgg cggctatggc gcctggcgcg ccaagacggt cgagatgacc
     3961 aagatgcccg agctggtggc gttcttccac agcatgatcg gtctggcggc ggtgttcatc
     4021 gccggcgcca cggtgatcga gccctgggcc ttcggcctgg ccaagagcgt cgcccagggc
     4081 ggcgacggcc agatcccggg cggcaaccgc atcgagctgg cgctgggcgc cttcatcggc
     4141 gcggtcacct tcagcggctc ggtgatcgcc tggggcaagc tgtcgggcaa gagcaagttc
     4201 cgcctgttcc agggcgcgcc ggtgcagttt gccggccagc actgggtcaa cctggggctg
     4261 ggcctgctga ccgcgttctt catcttcggc ttctggcatt cgcaaagcca cctggacttc
     4321 gccctggtgt gcctgctggg cttcatcatc ggcgtgacgc tgatcatccc gatcggcggc
     4381 gccgacatgc cggtggtggt gtcgatgctc aacagctact ccggctgggc ggcggcgggc
     4441 atcggctttt cgctcaacaa cagcatgctg atcattgccg gctcgctggt gggcagctcg
     4501 ggcgcgatcc tgagctacat catgtgcaag gccatgaacc gctcgttctt cagcgtcatt
     4561 ctgggcggct tcgggggtga ggcggtcacg gcgggcggcg cgcaggagca gcgcccggtc
     4621 aagagcggca gcgccgacga cgccgccttc atgctcggca acgccgacag cgtgatcatc
     4681 gtgccgggct atggcctggc ggtggcgcgc gcccagcatg cggtcaagga gctggtcgac
     4741 aagctcaccg acaagggcat cgacgtcaag tacgccatcc acccggtggc cggccgcatg
     4801 ccgggccaca tgaacgtgct gctggccgag gccgaagtgc cctacgacca ggtgcacgag
     4861 atggaagacg tcaacggcga gttcggccag gccgacgtgg ccatcatcct gggcgccaac
     4921 gacgtggtca acccggccgc gctgcaaaag ggcagcccga tctacggcat gccgatcctg
     4981 gaggcctaca aggccaagac cgtgatcgtc aacaagcgct ccatggccgc cggctacgcc
     5041 gggctggaca acgagctgtt ctacatggac aagaccatga tggtgttcgg cgacgccaag
     5101 aaggtggtcg aagacatcgg caaggccatc gagtaagtcc acgccggccg tcgctgcggc
     5161 gccacgaccg ggaaaacgcc ggtttgcccc tgcccgcgcg cgttgcacaa tgtgacaggt
     5221 ggccgcgcag cgcgccgcct tcccattgcc tgaccgtgcc gggcacggtc cgacctcaag
     5281 gagtacccca tccatgaccg agcgcagctg gctcaagcac taccccgccg gcgttcccgc
     5341 cgacatcgac accacccagt acagctccct ggtggcgctg atcgaggaga gcttctcgaa
     5401 gtacgccgat cgcaccgcct acagcttcat gggcaaggac gtcagctacg ccgacaccga
     5461 tgccgacagc cgcgctctgg ccgcctacct gcagagcctg ggcctgcagc gcggcgaccg
     5521 cgtggccctg atgatgccca acgtgccgca gtacccggcc agcgtggcgg cggtgctgcg
     5581 cgccggcttc gtggtggtca acgtcaaccc gctgtacacc gcgcgcgaac tcgagcacca
     5641 gctcaaggac gccggcgcca aggccatcat catcctggag aacttcgccg ccacgctgca
     5701 gcagtgcatc gccaggaccc aggtcaagca cgtggtgctg gccagcatgg gtgaccggct
     5761 gggcggcctc aagggcatga tcgtcaacct ggtggtgcgc aaggtcaaga aaatggtgcc
     5821 gccgttcagc ctgccgggcg cggtgcgctt caacgatgcg ctggcgcgcg ggcgcagcgc
     5881 gcccttcgcg gcgcccccga tcggccccga cgatctggcg gtgctgcagt acacgggcgg
     5941 caccaccggc gttgccaagg gcgccatgct gctgcaccgc aacctgatcg ccaacgtgct
     6001 gcaatccgag gcctggaacg acccggtgat gaagagcatc ccggccggcg agcagcccac
     6061 ctcggtgtgc gcgctgccgc tctaccacat cttcgccttc acggtgaaca tgatgctgtc
     6121 catgcgcact ggcggcaaga ccatcctgat ccccaacccg cgcgacctgc cggcggtgct
     6181 caaggagctg tccaagcaca ccttccacag ctttccggcg gtcaacacgc tgttcaacgg
     6241 cctggccaat cacccggact tcaacaccgt caactggaag aacctgcgcg tctcggtggg
     6301 cggcggcatg gcggtacaga gcgcggtggc caagctgtgg ctcgaaaaaa ccggctgccc
     6361 gatcgtcgaa ggctacggcc tgtccgaaac cagcccctcg gccagctgca acccggtcac
     6421 gtcgaaggag ttctccggca ccatcggcct gccgctgccc agcacctacc tcaagatcct
     6481 ggacgacagc ggggccgagg taccgctcgg tcagccgggc gaaatcgcca tcaagggccc
     6541 gcaggtgatg gccggctact ggcagcgccc cgacgagacc gccaaggtca tgacgcccga
     6601 cggctacttc aagagcggcg acatcggcat catggacgag cgcggctaca cacgcatcgt
     6661 cgaccgcaag aaggacatga tcctggtcag cggcttcaac gtctacccca acgagatcga
     6721 ggacgtggtg gccgccattc caggcgtgct ggaggtcggc gcggtcggcg tgcccgacga
     6781 aaagatgggc gaagccatca agctggtgat cgtcaagaag gaccccggac tgaccgagga
     6841 agcggtgcgc gcctattgca agcagaacct caccggctac aagcagccgc gcgtgatcga
     6901 gttccgcgcc gagctgccca agacgccggt cggcaaggtg ctgcgacgcg aactgcgcga
     6961 cccggccccg gccaaggcgg ccgccgctgc ggtggattga gcgccaccga cgccccgcgc
     7021 acgcatggcg ctgccccctg gcgcgagtga cggcggccgg ccgcgccgca ccggcgtggt
     7081 ggcggccatg catcccgaac tggcggccgt gctcgagcgc ctgccggacg agcagcgcca
     7141 gcgtgtggcc gggcgcgact tctgggtcgg tcactggcac gcccacgagg tggtggcggt
     7201 gctgtccggg gtgggcaagg tggcggcggc caccaccgcc acgctgctga tcgaacgctt
     7261 cggcgtcgac gccctgctgt tcaccggcac cgccggtggg ctgggcgacg gcgttcgggt
     7321 cggcgatctg gtgctggccg gcgagttcct gcagcacgac atggacgcct cgccgctgtt
     7381 tccgcgcttc gaggtgccgc tgtacggccg cgcgcgcttt gccaccgacg ctgcgctgtc
     7441 ggcgcgcctg ggcgccgccg cgcgccaggt gctggcccgc gccagcgaca cgctgggcgc
     7501 ccatgccgtg gccgaattcg ccctgcacgc gccggccgtg caccgcggcc tggtcgtcag
     7561 cggcgaccgc ttcgtcgcca ccagcgccga aagcgcggcg ctgcgccagg cgctgcccga
     7621 ggcgctggcg gtggaaatgg agggcgccgc cgtggcccag gtgtgtcacg actacgggct
     7681 gcccttcgcc gccctgcgca cggtgagcga ccgcgccgac gacagcgccc acgtcgattt
     7741 catgcgcttc atcgaccagg tggccagccg ctacagtgcc gcggtgctgg aagcgttttt
     7801 tgcagaacta tgatttcgat agctgctggc gcttgacccg agcgcgccag aggccgattt
     7861 gaccttcaaa ccgaggaatc acttcagcca gccacggcgg cggaaatacc acatcggcac
     7921 cagggcgctg gcgatcatca gcgcgatggc atagggatag ccgagctgcc agtccagctc
     7981 cggcatggcc ttgaagttca tgccgtagat gctggccacc agggtgggcg gcagcagggc
     8041 cacgctggcc accgagaaca gcttgatgat cttgttctgg ttgatgttga tgaagccgac
     8101 cgtggcgtcc atcaggaagt tgatcttgtc gaacaggaag gtggtgtggc cgtccagcga
     8161 atcgaggtcg cgctggatct ggcgcgcctc ctcgaactgc tcggcgtcca gcatgcggct
     8221 gcgcatcaga aagctgaccg cgcgccgcgt gtccatcacg ttgcggcgga tgcgcccgtt
     8281 catgtcctcc tgccgcgcga tggtggccag cacctcgctg gccacttcgt cggtcacgct
     8341 ttcgtccagc accatcttgc cggcggcttc cagctcgtcg tagatttcct ccagcgcgtc
     8401 ggccgagtat tcggcgtcgc cgtcgaacag cgccagcagc acgtccttgg cgtcttccag
     8461 caggcccggc atgcggcgcg cgcgcatgcg cagcagacga aacgccggca ccgattcgtc
     8521 gtggatcgaa aacagcacgc cgtggctgcg cagctggtcg ttcgactggt tcaggatgaa
     8581 ggccacgcgc agctggcgcg gcgtgtcgga gtcggccagc aggaagtcgc tgcggatgtg
     8641 cagctcgccg ttgtcttcct cgtagaagcg cgccgactct tccaggtcgt cgtcgatggc
     8701 gtcctcgggg atggacaggc cgtagtactg cttgacccag cgcttttctt ccggcgtggg
     8761 gtcttccaga tcgacccaga tcggccggta gcgcgacagg ctctccagcg cttcgatctc
     8821 ttcctgaaac aaccgcccgt tggacagcgt gaatacgttc agcatggcag gactccctgg
     8881 gcttgacggg gcggcgtggc ggaggtggcg gtggctttcg gccccccgtg ccagcgcatg
     8941 ccagggagac gaaagctagc gactcggggc gggttccaag gcgggctttc gtgcttgatg
     9001 atcgaagcct gtgattatcg catcggccgg cgccgcgtgg cgctggccgg ggcgggcagt
     9061 ttcttgacgg cgatcgcctg ccgcgatcgg caaaccggcg cagactggtt tagcatggca
     9121 tcgacgggca agccatgcca tgcgcgccgc ccgggcgggt cgcggaacgc ttccgcggtc
     9181 ctttgtcagt ggaagaagga ggttcttcat gaatatgacc atcagcggac accacctgga
     9241 aatcacgcct gcgctgcgca gctacgtgag caccaagctg gatcgggtga tccggcactt
     9301 cgatcaggtg gtggatgtca aggtgttact gtcggtcgag aaccagaagg aaaaggacaa
     9361 gcgtcagcgc gccgaatgcc gcatcggcgt caagggcaac gatctgtttg ccgagagcag
     9421 tcatgaagac ctgtacgccg ccgtggacga gctggtcgac aagctggatc gccaggtcgc
     9481 ccgctacaag gaaaagcagc agtcgcacga caagaacacc gccgtcaagc atttgatgta
     9541 ggatgctgta acaataacga gcggaaaccg tagactccag attgcaagcc gtcctgacgt
     9601 cgtcaggcgg ctttcttttt cggcggtgcg ccgccgcggt ctgaccgcac gcggcgcatc
     9661 tgggcggcaa caccctcgac gagcgtgaaa caccgcgtgc atttttggcg tcggcggcga
     9721 tttcgggccg ccgcggcggt caccggccat ccgtcggcgc ataatccgcg tccagaccca
     9781 tgaaccgtct cgcatccatc ctcccggagg cccaggtcct cgtcggcgtc gaagccacca
     9841 gcaagaagcg cgcgttcgag gaagccggcc tgctgttcga aagcctgcac ggtctgtcgc
     9901 gggcgctcat caccgacagc ctgttcgcgc gcgagcggct gggctcgacc ggcctgggcc
     9961 atggcgtggc cattccgcac ggccgcatca agggcctgaa ggcgcccatg gcggcggtgc
    10021 tgcggctggc gcagccgatc ggcttcgacg cgcccgacga acagcccgtc agcctgctga
    10081 tcttcctgct ggtgcccgaa gcggcgacgc aaaagcacct ggaaatcctg tccgaaatcg
    10141 ccgaactgct gagcgacgcc agcctgcgcg aacgcatcaa gggcgccacc tcggcacccg
    10201 agctgcacgg cctgatcgcc cactggcaat cgggccagcc ggtggcccag gcctgaagcg
    10261 cgccgccgcc atcgtccgcg ctgccccaga ccgagtgcgg cccacgccat gaagccttcc
    10321 gccatcagcg ccgacgtgct gttcgaagac caccgcgccg tactgcgctg gcagtggatg
    10381 gcggggctgg aagcgtcgga gcggcgcttc gacgagatcg ccgtgcgcgc cgcgcgctcc
    10441 ggcgccgacc tggtcggcta cctgaactac atccaccctt accgtgtgca ggtgctgggt
    10501 gagcgtgagg tcgcctacct gaccaacgcc tcgtccgagg actgcgcgcg ccgcatcgcc
    10561 cgcatcgtca cgctggagcc gccggtgctg gtgctgtgcg acggccagaa cccgcccgag
    10621 gcactgacgg cgctgtgcga gcgggcgcac attccgctgt ttgcgaccca ggaatcggcg
    10681 gccttcgtgg tcgacgtgct gcgcgcctac ctgtcgcgcc atttcgccga ccgcaccacc
    10741 atgcacggcg tgttcatgga catcctgggc ctgggcgtgc tgatcaccgg cgaatccggc
    10801 ctgggcaaga gcgagctggg gctggagctg atctcgcgcg gcaacggcct ggtggccgac
    10861 gacgcggtcg atctgtttcg catcaaccag accaccatcg aaggccgctg ccccgagctg
    10921 ctgcgcaacc tgctggaagt gcgcggcatc ggcctgctcg acattcgcgc catcttcggc
    10981 gaaaccgccg tgcgccgcca catgcgtctg cgcctgatcg tgcacttggt gcggcgcgaa
    11041 acgctggagc gcgactacga acgcctgccc tacgagccgc tgacccagga ggtgctgggc
    11101 gtgccgatcc agaaggtggt gatccaggtg gtggccggcc gcaacatcgc cgtgctggtg
    11161 gaggcggcgg tgcgcaacca catcctgcag ctgcgcggca tcgacaccta ccaggagttc
    11221 gtcgagcgcc accggcgcgc catggaacag ggcagcagcg cctgaacctg gaaacggcag
    11281 ttgatcgctt gtcaacttcg ggcagaccgc atcgatgcag ggtctgg
//
LOCUS       DAOQTZ010000086        11013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_6681_length_11013_cov_12.718744, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000086 DAOQTZ010000000
VERSION     DAOQTZ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6681_length_11013_cov_12.718744"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="PKN51_10240"
     CDS             <1..135
                     /locus_tag="PKN51_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875733.1"
                     /note="catalyzes the formation of pyruvate and
                     acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved
                     in the degradation of phenylpropionate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HNT85521.1"
                     /translation="YGVPAREILVEMGRRGMVGGQEDMIEDTALTLSKKKLAAQVASH
                     "
     gene            160..948
                     /gene="dmpH"
                     /locus_tag="PKN51_10245"
     CDS             160..948
                     /gene="dmpH"
                     /locus_tag="PKN51_10245"
                     /EC_number="4.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047437 - 4-oxalocrotonate decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-3-hexenedioate decarboxylase"
                     /protein_id="HNT85522.1"
                     /translation="MTLDPKTLDQLAEHLHACQREARDTLKITDQHPDMDWDDAYAIQ
                     DRILVAKLKGGARVAGYKAGLTSHAKMRQMGVTDPVFGFLVDEFTVADGGEVRVSELI
                     HPKVEPEICFVTRAELKGPGCHIGAVLAACDVVLPGIEVIDSRYRDFKFDVKSVIADN
                     TSAARFVVGGRAVDARRVDLTTCGLVLEKNGVPVALGAGAAVLGHPAAAVAMLVNHLG
                     RRGQSLPAGSLVLSGGATEAVSVQPGDHVSLRVQGMGSVSLRFV"
     gene            1008..1979
                     /locus_tag="PKN51_10250"
     CDS             1008..1979
                     /locus_tag="PKN51_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT85523.1"
                     /translation="MKRRDFIAAASAATLCHASWAQDAFPARPVKLIVPFPPGGPTDI
                     MGRTAAKAMGDRLGQQFVVENKAGAGGNIGTDAVAKAAPDGYTIGLAAISSLAIAPHL
                     YGKLPFNVDKDLAPISLVGTTPCAIMAGPQAPFSDLKGLIAYAKANPGKLSYATSGIG
                     TSNHLAAELLQSVAGIQLTHVPYKGSSQIVPDLIAGTVPMSMESSLATTLQHVRSGKL
                     KAIAVTSARRAKALPEVPTVAESGYAGFEVETWFGLIAPAATPAAIVSRLQGAWAEGS
                     RGADAQSAFDNISANLRVTTPAEFGAFIQAEHRRWGELIRKLGIKAD"
     gene            2150..2581
                     /locus_tag="PKN51_10255"
     CDS             2150..2581
                     /locus_tag="PKN51_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNT85524.1"
                     /translation="MDGDSLWVRPERGGRRARLRLQGIDAPELCQPGGASARAALQAL
                     VRGERLRVTVHARDRFGRRLASVQRQRDGLDVAAGMVAQGWAWNDRQGWRRGRYAREE
                     AQARAARRGVFAAADAQSPADFRRRHGPCGTAQSGAASTSR"
     assembly_gap    2686..2785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2863..3714)
                     /locus_tag="PKN51_10260"
     CDS             complement(2863..3714)
                     /locus_tag="PKN51_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNT85525.1"
                     /translation="MQPAFRSAAAALAAITLLGACTSTPHAPDSPAEVGELRPGAGYL
                     KGYLGREELPDSLALLPPPPADGTPAHADDADAFRQLTALQKGARGTLATRDANLKFP
                     QAAGAFACALGVTVSEQGTPHLNMLLRRTLTDAGLATYKAKDQYKRTRPFVTFSAPTC
                     TPAEEAALSKDGSYPSGHSALGWAWALVLTELAPERADALLLRGRSFGQSRGICGVHW
                     KSDIEAGRLIGAATVARLRVNAVFQAQLAAAKQEVVQARAAGQVPPAADCASETQTLR
                     SSLQLAP"
     gene            3897..6500
                     /gene="clpB"
                     /locus_tag="PKN51_10265"
     CDS             3897..6500
                     /gene="clpB"
                     /locus_tag="PKN51_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNT85526.1"
                     /translation="MRLDKLTTKFQQALADAQSLALGHDHAYIQPEHLLAAMLLQEDG
                     PRALLARAGVNVAALAQGIDAAVKRLPQVQGQEQVQPSPDLIKLLQATEKESIKRGDQ
                     FIPSEMFLLALSDAKGEAARIAKEHGLARASLEAAIDAVRGGQSMDSAEGEGQREALK
                     KYTLDLTERARQGKLDPVIGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLA
                     QRINAGEVPDTLRDKRVLVLDMAGLLAGAKYRGEFEERLKAVLKEVAAEEGRIILFID
                     ELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQKV
                     LVDEPSVEDTIAILRGLQERYEAHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLI
                     DEAAAKIKIEIDSKPEVMDKLDRRMIQLKIEREAVKKEKDEASQKHLAQIEEDLARLQ
                     KEYADLEEIWKAEKASAQGSEQVRKEIEQIKFQIQEATRKGDFNHVAELQYGKLPALE
                     KQLKEAQASEQGAEAQAKAHKLLRTQVGAEEIAEVVSRATGIPVSKMMQGERDKLLKM
                     EDALHQRVVGQDEAISAVANAIRRSRSGLSDPNRPLGSFLFLGPTGVGKTELTKALAG
                     FLFDSDQHMVRIDMSEFMEKHSVARLIGAPPGYVGYDEGGYLTEAVRRKPYSVVLFDE
                     IEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVLIMTSNIGSPMIQAMAGQPYEDV
                     KDAVFGELKNHFRPEFLNRIDEIVVFHGLDAAQIGAIAKIQLKVLAERLARMELTLNV
                     SDQALAELAKVGFDPVFGARPLKRAIQQRIENPLAKLVLEGKFPPKTEIEVSVDPIKD
                     PGVFQFSSHTG"
     gene            6593..7396
                     /locus_tag="PKN51_10270"
     CDS             6593..7396
                     /locus_tag="PKN51_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNT85527.1"
                     /translation="MTAANPSTPNLASLADELERTLKLRTPPFGMKLLASAESLKAIP
                     KLRRPQAVHTMDQIVAQAARLGWTLGVTAGDLVGDQCRAVVGLGGQDAAWYAGQPMAG
                     VWFATVEDSARHQAAMEVVPVGRHEALVVAPLAKWGGFGAGQDEPDIALFYATPGAMI
                     YFINGLQWSGYQRFDWSVVGESSCADSWGRALKTRQPSLSIPCFAERRYGGVLDDEML
                     MALPVADIARALAGMRALAKNGLRYPFAQYGIQQDVRAGMGVSYPKGSA"
     gene            7584..8816
                     /locus_tag="PKN51_10275"
     CDS             7584..8816
                     /locus_tag="PKN51_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT85528.1"
                     /translation="MSTSAVSAAADARVTVPLREDARVIGLVGLAHASSHFAHLLLPP
                     LFPVFATEFGLSFSQLGLLMTVFFVISGVGQAMSGFLVDRVGARPVLFGSLLCFIVAS
                     LSGYFAQGWGGLLLVAVFAGLGNAPFHPVDFSILNHRVSGPRLGYAFSAHGLSGNLGW
                     ALTPVFLLFFTELSSWRTGYLAAALLYAGVLAVLVLQREHLHTEAVVHHAHGDAQGGG
                     AGFLRLPVVWWCFAFFLLSTLTLAVVQNFASPIMKAMHGVSFEAATTMITAYMLCGAL
                     GMLVGGFVAARMPAASDHVVAWAMGVGALLIVAAASGWLGGFGSMAVLAGTGFAVGVG
                     GPSRDLMIKKATPKGATGRIYGTVYSGLDIGFAIGPLIYGVFMDRGMYATTLVLAALF
                     LALSVAAALGVGRRTRLA"
     gene            8836..10524
                     /locus_tag="PKN51_10280"
     CDS             8836..10524
                     /locus_tag="PKN51_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNT85529.1"
                     /translation="MTSRIAGHLLVDCLLAQGVSHVFGVPGESYLAVLDGFYAQRERI
                     RFVICRQEGGAAFMAEAHGKLTGRPGVCFVTRGPGATNASIGVHTAAQDSTPMVLFVG
                     DVASDQRDREAFQEVDYRAFFGAFGMAKRVERVDSPERLPEYVARAFATALNGRPGPV
                     VLVLPEDMLTQPVPDALQPQPRWQVPAWLPDAGGLARLQALLQAAQRPIVIAGGSSWT
                     PEGARELQAFAEAWQLPVATALRFQDSFDNHHRLYAGDVGIAINPRLAERIRQADLVL
                     ALGPRLGEMTTSGYTLLQVPRPQQRLIHVHPGAEELGRVYQPELALCASLPAMAAALA
                     ALPAPASVPWRDWTEAAHADYLANLQAQALPGPLDMAAVIGTLQRLLPPDAALCNGAG
                     NFASWAHRFFRYHGLTKGVKTQLAPTNGAMGYGVPAAIACAITSGRVVVALTGDGDFM
                     MTGQELATARQYGARLIVVLVNNGVYGTIRMHQARAYPQRQAGSMLTNPDFVRLAESH
                     GLAGVRVERTEQFEPALRAALARSDGTLIEIMLSEQVITTRSTLEAIETQAAGR"
     gene            complement(10648..>11013)
                     /locus_tag="PKN51_10285"
     CDS             complement(10648..>11013)
                     /locus_tag="PKN51_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HNT85530.1"
                     /translation="AKKAAPAKKAAPAKKAAPAKKAAPAKKAAPAKKAAPAKKRTPSA
                     AFMKAMTPSAALAAVIGSKALPRTEVTKKVWEYIKANKLQDSVNKRNINADAKLKPIF
                     GKAQVTMFEMTKLISNHLS"
BASE COUNT         1672 a         3695 c         3881 g         1665 t
ORIGIN      
        1 tacggcgtgc cggcgcgcga gatcctggtc gagatgggcc gccgcggcat ggtcggcggc
       61 caggaagaca tgatcgagga caccgccctg acgctatcaa aaaagaagct ggcggcgcag
      121 gttgcaagcc actgaaggcc aattgaagtt cacaacgcca tgacactcga cccgaagacg
      181 ctcgaccagc tggccgagca cctgcacgcc tgccagcgcg aggcgcgcga cacactgaag
      241 atcaccgacc agcaccccga catggactgg gacgacgcct acgccatcca ggaccgcatc
      301 ctggtcgcca agctcaaggg cggcgcgcgc gtggcgggct acaaggcggg cctgacctcg
      361 cacgccaaga tgcgccagat gggcgtgacc gacccggtgt tcggctttct ggtcgacgag
      421 ttcaccgtcg ccgacggcgg cgaggtcagg gtgagcgagc tgatccatcc caaggtggaa
      481 cctgagatct gtttcgtcac ccgcgctgag ctgaaaggcc ctggctgcca catcggcgcc
      541 gtgctggccg cctgcgatgt ggtgctgccc ggcatcgaag tcatcgacag ccgctaccgc
      601 gacttcaaat tcgacgtgaa aagcgtgatt gcagacaaca cctcggccgc gcgcttcgtc
      661 gtcggtggtc gtgcggtgga cgcgcgccgc gtcgatctca cgacctgcgg cctggtgctg
      721 gagaaaaacg gcgtgccggt ggccctgggc gccggcgccg ccgtgctggg ccacccggcc
      781 gccgccgtgg ccatgctggt caaccacctg ggccggcgcg gccagagcct gccggccggc
      841 agcctggtgc tgtcgggcgg cgccaccgag gcggtgtcgg tgcagccggg cgaccacgtc
      901 agtctgcgcg tgcagggcat gggcagcgtg tcgctgcgct tcgtctgaaa ctcagccccg
      961 ggcgccggcc agacaggcgc ccatcgaccg acgcaccgga gaccttcatg aaacgacgcg
     1021 acttcatcgc cgcggcgagc gccgccacgc tgtgccacgc aagctgggcg caggacgcct
     1081 ttccagcccg cccggtcaag ctgatcgtgc cgttcccgcc gggcggtccg accgacatca
     1141 tgggtcgcac cgccgccaag gccatgggcg acaggctggg ccagcagttc gtggtcgaga
     1201 acaaggccgg cgccggtggc aacatcggca ccgacgccgt ggccaaggcg gcgcccgacg
     1261 gctacaccat cggcctggcg gccatcagca gcctggccat cgcgccgcac ctgtacggca
     1321 agctgccgtt caacgtcgac aaggacctgg cgccgatctc gctggtgggc accacgccct
     1381 gcgccatcat ggccggtccg caggcaccgt tctcggacct gaagggcctg atcgcctacg
     1441 ccaaggccaa ccccggcaag ctcagctacg ccacctcggg catcggaacc agcaaccatc
     1501 tggcggcgga actgctgcag tcggtcgccg gcattcagtt gacgcatgtc ccctacaagg
     1561 ggtcgagcca gatcgtgccc gacctgatcg ccggcacggt gccgatgagc atggaaagct
     1621 cgctggccac caccttgcag catgtgcgct cgggcaagct caaggccatt gccgtcacct
     1681 cggcccggcg tgccaaggcc ctgcccgagg tacccacggt ggccgagtcc ggctatgcgg
     1741 ggttcgaagt ggagacctgg ttcggtctga tcgccccggc ggccacgcct gccgccatcg
     1801 tgtcccgtct gcagggcgcc tgggccgaag gctcgcgcgg ggccgacgcc cagagcgcgt
     1861 tcgacaacat ctcggccaac ctgcgcgtca ccacgccggc cgagttcggc gccttcatcc
     1921 aggccgagca caggcgctgg ggtgagctga ttcgcaagct gggcatcaag gccgattgat
     1981 ggggggtgcc gcgggccggc atgtgccagc gcgtcgcctg ggccgcgcgg cctgcgcggc
     2041 gctgctgctg tccctgggtg cgttgcgggg tgctgccgag cccgcgcggg cgctgggcgg
     2101 cgaagcagct gccgccggac cgtcctggca cgcgcgtgtg gagtttgtcg tggatggcga
     2161 ttcgctgtgg gtgcggccgg agcgcggcgg tcggcgcgct cggctgcgcc tgcagggcat
     2221 cgacgcgccc gagctgtgcc agccgggcgg cgcgtcggcg cgtgcggccc tgcaggcgct
     2281 ggtgcgtggc gagcggctgc gcgtgaccgt gcatgcccgg gaccggttcg ggcgtcgcct
     2341 ggcgagcgtg cagcgccagc gcgacgggct ggatgtggcg gcgggcatgg tggcccaggg
     2401 ctgggcctgg aacgaccgcc agggctggcg ccgcggccgc tacgcacgcg aggaggcgca
     2461 ggcccgcgcg gcccggcgcg gcgtgttcgc cgccgccgat gcccagtcgc cggccgactt
     2521 tcggcgccgc cacggaccgt gcgggaccgc gcagtctggt gccgcgtcga cgtccagata
     2581 gctacagaaa taatagcttc tggcgctgat ccggagcgcc gttctacgcc aatcgatgcc
     2641 caacaccggc gaccggcgac cggcgaccgg cgaccggcga ccggcnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnaatcg atgcccaaca ccggcgaccg gcgaccggcg
     2821 accggcgacc ggcgaccggc caccggccac cggccaccgg cgtcaaggcg cgagctgcaa
     2881 ggacgagcgc agcgtctggg tctctgacgc gcagtccgcg gccggcggca cctggcccgc
     2941 ggcgcgggct tgcacgactt cctgcttcgc ggctgccagc tgggcctgga agaccgcatt
     3001 gacccgcagg cgcgccacgg tggcggcgcc gatcaggcgg ccggcctcga tgtcgctctt
     3061 ccagtgcacg ccgcagatgc cgcggctctg gccaaaggag cgcccgcgca gcagcagggc
     3121 atcggcgcgc tccggcgcca gttcggttag caccagggcc caggcccagc ccagcgcgga
     3181 atggccggat gggtagctgc cgtccttgct cagggccgcc tcctcggcgg gggtgcaggt
     3241 gggggcgctg aatgtcacga acgggcgcgt gcgcttgtac tggtccttgg ccttgtaggt
     3301 ggccagaccg gcgtcggtca gcgtgcgccg cagcagcatg ttcaggtgcg gcgtgccctg
     3361 ttccgagacc gtgacgccca gggcgcaggc aaaggcaccg gccgcctgtg ggaacttcag
     3421 gttggcatcg cgcgtggcca gcgtgcccct tgcccccttc tgcagggcgg tcagctgccg
     3481 gaatgcgtcg gcatcgtcgg catgggccgg cgtgccatcc gcaggcggag gtggcagcag
     3541 tgccaggctg tcgggcagct cctcgcggcc gaggtaaccc ttgaggtagc ctgccccggg
     3601 gcgcagttcg cccacctcgg cgggggaatc cggcgcgtgc ggcgtgctgg tgcacgcgcc
     3661 gagcagcgtg atcgcggcca gagccgctgc ggcggaccgg aaggcaggtt gcatgggtgt
     3721 ctcccgtgga ggtgttgacg gcttcggcct ttatggcccg ctggcagtgt agcggtgtgc
     3781 tatggacagt ctttctttgt gcatcgtgcg atcgttgaag agaatccacg cggccggccc
     3841 agacgccttg aatccggggc agacctcccc atttgatggg caattcggag gttttcatgc
     3901 gcctagacaa gctcaccacc aagttccagc aggccctggc cgacgctcag tcgctggccc
     3961 tgggtcacga ccacgcctac attcaacccg agcacctgtt ggcagccatg ctgctgcagg
     4021 aagacggtcc gcgcgcgttg ctggcgcgtg ccggggtcaa cgtggccgcg ctggcccagg
     4081 gcatcgacgc cgccgtcaag cgcctgccgc aggtacaggg ccaggagcag gtgcaaccca
     4141 gcccggacct gatcaagctg ctgcaggcca ccgagaagga gagcatcaag cgcggcgatc
     4201 agttcatccc cagcgagatg ttcctgctgg ccctgagcga cgccaagggc gaggccgcgc
     4261 gcattgccaa ggaacacggc ctggcgcgcg ccagtctgga agcggcgatc gacgccgtgc
     4321 gcggcggcca gagcatggac agcgccgagg gagagggcca gcgcgaggcg ctgaagaaat
     4381 acacgctcga tctgaccgag cgcgcgcgcc agggcaagct ggacccagtg atcggccgcg
     4441 acgatgaaat caggcgcacg atccaggtgt tgcagcggcg cagcaagaac aacccggtgc
     4501 tgatcggcga gccgggcgtg ggcaagaccg ccatcgtcga gggcctggcg cagcgcatca
     4561 acgccggcga agtgcccgac accctgcgtg acaagcgcgt gctggtgctc gacatggccg
     4621 gcctgctggc cggcgccaag taccgcggcg aatttgaaga gcgcctgaag gccgtgctca
     4681 aggaagtggc ggccgaagag ggccgcatca ttctgttcat cgacgagctg cacaccatgg
     4741 tcggcgccgg caaggccgag ggcgcgatcg acgccggcaa catgctcaag ccggcgctgg
     4801 cgcgtggcga gctgcactgc gtcggcgcca ccacgctgga cgaataccgc aagtacatcg
     4861 agaaggacgc ggcgctggag cggcgcttcc agaaggtgct ggtggacgag cccagcgtgg
     4921 aagacaccat cgccatcctg cgcggcctgc aggagcgtta cgaggcgcac cacggcgtcg
     4981 acatcaccga cccggccatc gtggccgcgg ccgagctgag ccaccgctac atcaccgacc
     5041 gcttcctgcc cgacaaggcg atcgacctga tcgacgaggc ggcggccaag atcaagatcg
     5101 aaatcgattc caagcccgag gtgatggaca agctcgatcg ccgcatgatc cagctcaaga
     5161 tcgagcgcga ggccgtcaag aaggaaaagg acgaggcttc gcagaagcac ctggcgcaga
     5221 tcgaggaaga cctggcgcgg ctgcagaagg aatacgccga cctggaagaa atctggaagg
     5281 ccgaaaaagc cagcgcccag ggcagcgagc aggtgcgcaa ggagatcgag cagatcaagt
     5341 ttcagatcca ggaagccacg cgcaagggcg acttcaacca cgtcgccgag ctgcagtacg
     5401 gcaagttgcc ggcgctggaa aagcagctca aggaagcgca ggccagcgag cagggcgccg
     5461 aggcccaggc caaggcgcac aagctgctgc gcacccaggt cggtgccgag gagatcgccg
     5521 aagtggtcag ccgcgccacc ggcatcccgg tcagcaagat gatgcagggc gagcgcgaca
     5581 agctgctgaa gatggaagac gcgctgcacc agcgcgtggt gggtcaggac gaagccatct
     5641 cggccgtggc caacgccatc cgccgctcgc gctcgggcct gtcggacccg aaccgcccgc
     5701 tcggctcctt cctgttcctc ggccccacgg gtgtcggcaa gaccgaactg accaaggcgc
     5761 tggcgggttt cctgttcgac agcgaccagc acatggtgcg catcgacatg agcgaattca
     5821 tggaaaagca cagcgtcgcg cgcctgatcg gcgcgccgcc gggctacgtg ggctatgacg
     5881 agggcggcta cctgaccgaa gcggtgcggc gcaagcccta cagcgtggtg ctgttcgacg
     5941 agatcgaaaa ggcccacccc gacgtcttca acgtgctgct gcaggtgctc gacgacgggc
     6001 gcctgaccga cggccagggc cgcacggtgg acttcaagaa caccgtgctg atcatgacca
     6061 gcaacatcgg cagcccgatg atccaggcca tggccggcca gccctacgaa gacgtgaagg
     6121 acgcggtgtt cggtgagctg aagaaccact tccgccccga attcctcaac cgcatcgacg
     6181 agatcgtcgt cttccacggc ctggacgcgg cgcagatcgg ggcgatcgcc aagatccagc
     6241 tcaaggtgct ggccgagcgg ctggcgcgca tggagctgac gctgaacgtg tccgaccagg
     6301 cgctggccga gctggccaag gtcgggttcg acccggtgtt cggcgcccgg ccgctcaagc
     6361 gcgccatcca gcagcgcatc gagaatccgc tggccaaact ggtgctggag ggcaagtttc
     6421 cgcccaagac cgagatcgag gtgtcggtcg atccgatcaa ggatccgggg gtgtttcagt
     6481 tcagcagcca caccggctga agaatcagcc accgcgcgac gatcgcgaca cacaccagcg
     6541 gggacgtgcg ccacaatggc cacgtccctt ttttttgtgc cagagtaccg ccatgaccgc
     6601 tgccaatccg tccacgccca atctggccag cctggccgac gaattggagc gcacgctcaa
     6661 gctgcgcacg ccgccgttcg gcatgaagct gctggcgtcg gccgaatcgc tgaaggccat
     6721 tcccaagctg cgccgcccgc aggccgtcca caccatggac cagatcgtcg cccaggcggc
     6781 gcgcctgggc tggacgctgg gcgtcaccgc cggcgacctg gtgggcgatc aatgccgcgc
     6841 cgtggtcggc ctgggcgggc aggacgccgc gtggtatgcc ggccagccga tggcgggcgt
     6901 gtggttcgcc acggtggagg actcggcccg ccaccaggcg gcgatggagg tggtgccggt
     6961 gggccgccac gaggcgctgg tggtggcgcc gctggccaag tggggcggct ttggtgccgg
     7021 gcaggacgag cccgacatcg cgctgttcta cgccacgcct ggggccatga tctacttcat
     7081 caacggcctg cagtggtcgg gctaccagcg cttcgactgg agcgtggtgg gcgagtcttc
     7141 ctgcgccgac tcctggggcc gggcgctcaa gacgcgccag cccagcctgt cgatcccctg
     7201 ctttgccgag cgccgctacg gcggcgtgct ggatgacgag atgctgatgg cgctgcctgt
     7261 cgccgacatc gccagggcgc tggccggcat gcgggcgctg gccaagaacg gcctgcgcta
     7321 tccgtttgcg caatacggca tccagcagga cgtgcgcgcc ggcatgggtg tgagctaccc
     7381 caagggcagc gcctgagctt gcaaagggcg ggtggggcgg ccgcgccgca gcccggtcag
     7441 cgtctgaatt gctatgatag tgatagctgc tggcgcttga taggtgggcg cggcagcttg
     7501 ttttattgca tgtccccagg cgctgccccg gtgcttgcgc ggcgcgcggc ggggcgtgtc
     7561 acaaacgcgt accattcgcg tccatgtcca cttctgccgt ctccgcggcc gccgatgcgc
     7621 gggtcaccgt gcccctgcgc gaagacgcgc gggtgatcgg gctggtcggt ctggcccacg
     7681 ccagctcgca ctttgcgcac ctgctgctgc cgccgctgtt tccggtgttc gccaccgagt
     7741 tcgggctgtc gttttcgcaa ctgggcctgt tgatgacggt gtttttcgtc atctccggcg
     7801 tcgggcaggc catgtcgggc tttctggtcg accgggtggg cgcgcggccg gtgctgtttg
     7861 gctcgctgct gtgcttcatt gtggccagcc tgtcgggcta cttcgcccag ggctggggcg
     7921 ggctgctgct ggtggcggtg tttgccggcc tgggcaacgc gccgtttcac ccggtggatt
     7981 tcagcatcct caatcaccgt gtgtcggggc cgcgactggg atacgccttc agcgcccacg
     8041 gcctgtcggg caacctgggc tgggcgctga cgccggtgtt cctgctgttc ttcaccgagc
     8101 tgtcgagctg gcgcaccggc tacctggcgg cggcgctgct gtacgccggc gtgctggcgg
     8161 tgctggtgct gcagcgcgaa cacctgcaca ccgaggcggt ggtgcaccac gcgcacggcg
     8221 acgcacaggg tggcggcgcc ggctttctgc gcctgccggt ggtctggtgg tgcttcgcct
     8281 tctttctgct gtccacgctg acgctggcgg tggtgcagaa cttcgcctcg cccatcatga
     8341 aggccatgca cggcgtcagc ttcgaggcgg ccaccaccat gatcaccgcc tacatgctgt
     8401 gcggcgccct gggcatgctg gtgggcggct tcgtggccgc gcgcatgccg gcggcgtccg
     8461 accacgtggt ggcctgggcc atgggcgtgg gcgcgctgct gatcgtggcg gcggccagcg
     8521 gctggctggg tggcttcggc tccatggcgg tgctggcggg caccggtttc gcggtcggcg
     8581 tgggcggccc cagccgcgac ctgatgatca agaaagccac ccccaagggt gccaccgggc
     8641 gcatctacgg cacggtgtat tcggggctgg acatcggctt tgccatcgga ccgctgatct
     8701 acggcgtctt catggaccgc ggcatgtacg ccaccacgct ggtgctggcg gcgctgttcc
     8761 tggcgctgtc ggtggcggcg gcgctgggtg tgggtcgccg cacccgtctg gcttgagttc
     8821 aagcacaatg ccgcgatgac ctctcgcatc gccggccacc tccttgtcga ttgcctgctg
     8881 gcccagggcg tcagccacgt ctttggcgtg ccgggggaga gctacctggc ggtgctggac
     8941 ggcttttacg cccagcgcga gcgcatccgg ttcgtgatct gccgccagga aggcggcgcc
     9001 gccttcatgg ccgaagcgca cggcaagctc accgggcggc cgggcgtgtg cttcgtcacc
     9061 cgcgggccgg gcgccaccaa cgccagcatc ggtgtgcaca cggcggcgca ggactcgacg
     9121 ccgatggtgc tgttcgtcgg cgacgtggcc agcgaccagc gcgatcgcga agcctttcag
     9181 gaggtcgact accgcgcctt cttcggcgcc ttcggcatgg ccaagcgggt cgagcgcgtc
     9241 gacagccccg agcgcctgcc cgaatacgtg gcgcgagcct ttgccaccgc cctcaacggc
     9301 cgtcccggcc cggtggtgct ggtgctgccc gaagacatgc tgacgcagcc ggtgcccgac
     9361 gcgctgcagc cgcagccacg ctggcaggtc ccggcctggc tgcccgacgc cggcgggctg
     9421 gcgcgcctgc aggcgctgct gcaggcggcg cagcgtccga tcgtcatcgc cgggggcagc
     9481 agctggacgc ccgagggcgc gcgcgagctg caggcctttg ccgaggcctg gcagctgccg
     9541 gtggcgacgg cgctgcgctt tcaggacagc ttcgacaacc accaccggct gtacgcgggc
     9601 gacgtcggca tcgccatcaa cccgcggctg gccgagcgca tccgccaggc cgatctggtg
     9661 ctggcgctgg ggccgcgcct gggcgagatg accaccagcg gttacaccct gctgcaggtg
     9721 ccgcggccgc agcagcgcct gatccatgtg caccccggcg ccgaggagct gggccgtgtg
     9781 taccagcccg aactggccct gtgcgccagc ctgccggcca tggccgccgc gctggcggcg
     9841 ctgccggcgc ccgccagcgt gccgtggcgc gactggaccg aggcggcgca cgccgactac
     9901 ctggccaacc tgcaggccca ggcgctgccc gggccgctgg acatggcggc cgtgatcggc
     9961 accctgcagc gcctgctgcc gccggacgct gccctgtgca acggcgccgg caactttgcc
    10021 agctgggcgc accggttctt ccgctaccac ggcctgacca agggcgtgaa gacccagctg
    10081 gcgcccacca acggcgccat gggctatggc gtgccggcgg ccatcgcctg cgccatcacc
    10141 agcgggcggg tggtggtggc gctgaccggc gacggcgact tcatgatgac cgggcaggag
    10201 ctggccaccg cgcgccagta cggcgcccgg ctgatcgtgg tgctggtcaa caacggcgtc
    10261 tacggcacca tccgcatgca ccaggcgcgc gcctacccgc agcgccaggc cggctcgatg
    10321 ctgaccaacc ccgacttcgt gcggctggct gaaagccacg gactggccgg cgtgcgggtg
    10381 gagcgcacgg agcagttcga acccgccctg cgcgcggcgc tcgcccgcag cgacggtacc
    10441 ctgatcgaga tcatgctgag cgagcaggtc atcaccaccc gcagcacgct ggaggcgatt
    10501 gaaacgcagg ccgccgggcg ttgacgatgc tccgttgttg gtagctgcca gcgcttgtca
    10561 gcaggctgct gcaggccgat aaggcataaa aaaaccggcc cctgggggcc ggttttcgcc
    10621 gttctggccc caagggccag acgtcgatca cgacaggtga ttgctgatca gcttggtcat
    10681 ctcgaacatg gtgacctggg ccttgccgaa gatcggcttc agcttggcgt cggcgttgat
    10741 gttgcgcttg ttgacgctgt cctgcagctt gttggccttg atgtactccc acaccttctt
    10801 ggtgacctcg gtgcgcggca gcgccttgct gccgatcacg gcggccagcg cggcgctggg
    10861 cgtcatggcc ttcatgaagg cggcgctggg ggtgcgcttc ttggccgggg cggccttctt
    10921 ggcaggtgcc gccttcttgg ccggagcggc cttcttggcg ggtgccgcct tcttcgcggg
    10981 ggccgccttc ttggcgggtg cggccttctt ggc
//
LOCUS       DAOQTZ010000087        10761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_6884_length_10761_cov_12.917523, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000087 DAOQTZ010000000
VERSION     DAOQTZ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6884_length_10761_cov_12.917523"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKN51_10290"
     CDS             complement(<1..284)
                     /locus_tag="PKN51_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT85531.1"
                     /translation="MTTSQNPTARALALAADDMRQVDAIISRRLDSDVPLVGQVAGYI
                     VSSGGKRLRPVLLLLMCQALGCLHPQRHTLAAVVEFIHTATLLHDDVVDES"
     gene            485..814
                     /gene="rplU"
                     /locus_tag="PKN51_10295"
     CDS             485..814
                     /gene="rplU"
                     /locus_tag="PKN51_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNT85532.1"
                     /translation="MYAVIKTGGKQYRVAAGEKIKVEQIAADVGQEITLDQVLAVGNG
                     SELQIGAPLVSGATVVAKVLSHGKHDKVRIFKMRRRKHYQKRQGHRQGYTELEIGAIR
                     GAGASKE"
     gene            821..1081
                     /gene="rpmA"
                     /locus_tag="PKN51_10300"
     CDS             821..1081
                     /gene="rpmA"
                     /locus_tag="PKN51_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNT85533.1"
                     /translation="MAQKKGGGSTRNGRDSKPKMLGVKVFGGQTVSAGSIIVRQRGTK
                     FHAGSNVGVGKDHTLFALVDGQVSFATKGALNKHVVQVTPAA"
     gene            1172..2251
                     /gene="obgE"
                     /locus_tag="PKN51_10305"
     CDS             1172..2251
                     /gene="obgE"
                     /locus_tag="PKN51_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNT85534.1"
                     /translation="MKFVDEAFIDVAAGDGGNGCSSFRREKFREFGGPDGGNGGRGGH
                     VFALADPNLNTLVDFRYARRHEARRGQHGMGSDMFGAAGDDITLRMPVGTIISDADTG
                     EVLHELLQPGQAVLVAKGGDGGFGNLHFKSSTNRAPRQKTPGWPGEKRSLKLELKVLA
                     DVGLLGMPNAGKSTFIAAVSNARPKIADYPFTTLYPNLGVVRVGPEQSFVVADIPGLI
                     EGASEGAGLGHQFLRHLQRTRLLLHIVDIAPFDEGVDPVVQARAIVAELKKYDPSLHA
                     KPRWLVLNKLDMVPPAEREARVREFVRRLRWKGPVFQISALTREGCEPLIQAVYQFVR
                     AQQVADAPAEPPDPRFADAAEPHQK"
     gene            2291..3436
                     /gene="proB"
                     /locus_tag="PKN51_10310"
     CDS             2291..3436
                     /gene="proB"
                     /locus_tag="PKN51_10310"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNT85535.1"
                     /translation="MTQNSVSEVLRQAQRIVIKVGSSLVTNEGRGLDETAIGEWCRQM
                     ARLAGQADLPREVVMVSSGAIAEGMKRLGWTQRPSEVNELQAAAAVGQMGLAQMYETK
                     LREQGLRSAQVLLTHADLADRERYLNARSTLLTLLEHRVLPVINENDTVVTDEIKVGD
                     NDTLGALVANLIEADLLVILTDQKGLYTADPRANPQAEFVHEARAGDPALERMAGGAG
                     SSIGKGGMLTKILAAKRAARSGASTVIAWGREPDVLLRLSRGERIGTLLVAPTQKLQA
                     RKQWMADHLQLRGALVIDDGAVAKLRGAGKSLLPIGMTQVEGAFARGDVVAIRDARGA
                     EVARGLANYSSAEARLLCRRPSGDITVLLGYMAEPEMVHRDNMVLIR"
     gene            complement(3488..4030)
                     /locus_tag="PKN51_10315"
     CDS             complement(3488..4030)
                     /locus_tag="PKN51_10315"
                     /inference="COORDINATES: protein motif:HMM:NF020331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HNT85536.1"
                     /translation="MSRRLRPPGRRAWLACLLAACAGLFSAPGAAQYADLDRADWQED
                     PVPPPPAYSLERLLEIDMPKTASVRMGIDPQTLSVNHQSGVVRYVVVARGTSAVNASY
                     EGIRCTTGEFRVYARQVQGGEWTPSTDSGWKSMRGQSSVLVQHPLRLARDGLCLGPSA
                     RQTVSEMVRELKTGNRSLYY"
     gene            complement(4027..4677)
                     /locus_tag="PKN51_10320"
     CDS             complement(4027..4677)
                     /locus_tag="PKN51_10320"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNT85537.1"
                     /translation="MLDREGFRPNVGIILLNQKNQVFWGKRIRTHSWQFPQGGIDRGE
                     SPEQAMYRELHEEVGLQPEHVRIVARTRDWLRYEVPDRFIRRDARGHYKGQKQIWYLL
                     QLIAQDWQLNLRATSHPEFDAWRWNDYWVPLDVVVEFKRGVYEMALTELARYLPRTEN
                     RNRYLRGGVRARDGGELPAIQTRDRLARAASAAIALPPGATLEGVPRPPFGPPDKP"
     gene            4785..6530
                     /locus_tag="PKN51_10325"
     CDS             4785..6530
                     /locus_tag="PKN51_10325"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNT85538.1"
                     /translation="MKASQFYISTLKEAPADAEIVSHQLMTRAGMIKKLGAGIYTYMP
                     MGLRVIRKVEAIVREEMNRAGAVECVMPVVQPGELWSESGRFQTMGPELMRIRDRHER
                     DFVVQPTSEEAVTDIARQDLRSYKQLPRNLYQIQTKFRDERRPRFGLMRGREFIMKDA
                     YSFDRDEPGAKRSYQAMARAYRAIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAATGE
                     DAIVYCPGSDYAANMEKAEALAPAGPRPAPAQALTNTPTPGKATCAEVAELLGLPLAR
                     TVKSLVLATDELDAQGAVAKTQIWLLLLRGDHDMNEIKAAKVPGLNAGFRFATVPEIQ
                     AHFGTPPGYLGPIGLKRPVKVVADREVALMADWVCGANAEGHHLTGVNWGRDLPEPDV
                     VADLRNVVAGDASPDGRGVLAIERGIEIGHIFYLGTKYSRAMNATFLDEDGKPKPFEM
                     GCYGIGITRLPAAAIEQNHDARGIIWPDAIAPFTVVICPVGMDRSESVKTAAEALYAE
                     LLAAGVDVILDDRGERPGVMFADWELIGVPHRVTIGDKGLKDGQVEYQHRRDSTATRV
                     PVDGIAAFVTGRLAV"
     gene            6602..7180
                     /locus_tag="PKN51_10330"
     CDS             6602..7180
                     /locus_tag="PKN51_10330"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNT85539.1"
                     /translation="MPGALIFQERALAGGQLEEPLADAVRTALSSAIANDAPPLPVFA
                     STDARLRYLRWLSGMSDRLYSRKKDFNARIEFLQTVWYESQRAGLETSLVLGLIQVES
                     AFRKYAVSSVGARGYMQVMPFWSRVIGDGDAGKLFHMQTNLRFGCVILRHYLERERGD
                     LFMALGRYNGSRGRPQYPNAVFGAQKRWVLSA"
     gene            complement(7220..8521)
                     /locus_tag="PKN51_10335"
     CDS             complement(7220..8521)
                     /locus_tag="PKN51_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNT85540.1"
                     /translation="MSLTQSLLLIALLIAVSAFFSLAEIALAASRRLRLRQLADDGDA
                     RALRVLRVQEQPGFYFTTVQIGLNAVAILGGIVGEGLLGPYLAELLRLWLPERTAANL
                     SLVGSVAAVTSLFVVFADLVPKRLGMAEPERVAMAMVGPMGHVLLLFKPIVWLYTACT
                     DLVMRLLGLPMQRDERITPDDILALAEAGTQAGVLDRPGQQVIENVFELDTRTVTSAM
                     TPRDRIAWLRHDADDAEIRLRIAAEPFSTYPVCEGELDRVLGYVDAKDLFQRVLHQRP
                     IELTEPALLHKVVVVPDSLSLSEVLHQFRQQHEDFALIVNEYSTVVGVVTLNDVMSTV
                     MGELVTPEDEEPIVLRDDGSWLIDGHTPMADVQRALNLDEPPSAEAYDTLAGFLMVML
                     RRVPRRTDSVQWGGHRFEVMDVDNYRIDQVLVTRSERSAPE"
     gene            8755..9792
                     /locus_tag="PKN51_10340"
     CDS             8755..9792
                     /locus_tag="PKN51_10340"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNT85541.1"
                     /translation="MFDVYIREAGARFGLGEQALTVAQMLLADMTSPDTGGLMGFLER
                     FKSAGLGPVIQSWLGGGPSAQPISNSQLETVLGASGGLLSRLTGLLDAPRDNVTTAIG
                     YLLPAFVGRLTPGGSLPSSLPPEVLSLAAAGRDLLTAPAAASAPVAAGQPGMLRWLPW
                     VIVAAAALIGLAYCGKQEQATPSAPATPAVDAASTAAPAAGASAAADAASPAAPSASP
                     AAAAASGPEPGAAVEALTDAASRPALQVFFDSGKAEVHPDLAARAADLLAYVKANQGV
                     QVVIAGFNDPTGDAAANAELSRQRAQAVQAALVAAGLAAERTVLEKPADTTATTSSLA
                     AARRVDVTLRP"
     gene            complement(9862..10188)
                     /gene="grxD"
                     /locus_tag="PKN51_10345"
     CDS             complement(9862..10188)
                     /gene="grxD"
                     /locus_tag="PKN51_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNT85542.1"
                     /translation="MDQTTQQHIEGLVKGNDIVLFMKGDANFPMCGFSGRAVQILKAC
                     GVDTSAVRTVNVLDDEAVRQGIKEYSQWPTIPQLYVRGEFIGGSDIMMEMYESGELQQ
                     LVGGKS"
     gene            complement(10400..10693)
                     /locus_tag="PKN51_10350"
     CDS             complement(10400..10693)
                     /locus_tag="PKN51_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HNT85543.1"
                     /translation="MATYQELLKQQAALAVQVEEARKREIADAVARVRQIVNEYDLTE
                     KDVFPAGRRAATPRMAGKVAPKYRDPATGQTWTGRGKPPKWIDGKDRNLFRIG"
BASE COUNT         1739 a         3607 c         3739 g         1676 t
ORIGIN      
        1 gattcgtcga ccacgtcgtc gtgcagcagg gtggcggtgt ggatgaactc gaccaccgcc
       61 gccagcgtgt gacgctgcgg gtggaggcag ccgagcgcct ggcacatcag cagcagcagc
      121 accggccgca ggcgcttgcc gccggaagag acgatgtagc ctgccacctg gcccaccagg
      181 ggcacgtcgg aatcgagccg gcgcgaaatg atggcgtcga cctgtcgcat gtcgtcggca
      241 gccagggcca gcgcacgcgc ggtcgggttc tgggaggtgg tcaagagcgg caggggcgtg
      301 ggacttcggg cgtggcgtgc gcggcacgcc acgagcggcg ccgattatag gtttgcggtg
      361 gctggcggct gccccgggcg cgctcctacg gagccgggct gggcggcatt cgcgcagatc
      421 gctataatgc ccggttccgc cagaaatccg tctggtggat caatcgatca aaggacttga
      481 gatcatgtac gcggtcataa aaaccggtgg caagcagtat cgcgtggctg ccggcgaaaa
      541 aatcaaagta gaacagattg ctgcggacgt tggccaggaa atcacgctgg atcaggtgct
      601 ggctgtcgga aacggcagcg agttgcagat cggcgctccc ctggtgtccg gcgccaccgt
      661 cgtggccaag gtgctgagcc atggcaagca cgacaaggtg cgcatcttca agatgcgtcg
      721 ccgcaagcac tatcagaagc gccagggcca tcgccagggc tataccgaac tcgagatcgg
      781 cgccatccgc ggcgccggtg cgtccaagga gtaacacacc atggcacaga aaaaaggcgg
      841 cggctccacg cgcaacggcc gcgattccaa gcccaagatg ctgggcgtga aggtgtttgg
      901 cggccagacg gtgagcgccg gctcgatcat cgtgcgccag cgcggcacca agttccatgc
      961 cggctccaac gtcggcgtcg gcaaggacca caccctgttc gccctggtgg acggtcaggt
     1021 gtcgttcgcc accaagggcg cgctcaacaa gcacgtcgtg caggtgacgc ctgccgcttg
     1081 aggcactttt cccgtgcgtc caggccacaa gccccgcccg ccggcggggc tttggtgttt
     1141 ctgacgcagg cccgaccgac cggagcgccg catgaaattc gttgacgaag ccttcatcga
     1201 cgtggctgcc ggcgacggcg gcaacggctg ctcctcgttc cggcgcgaga aattccgcga
     1261 gttcggcggc cccgacggcg gcaatggcgg gcgcggcggg catgtgttcg cgctggccga
     1321 ccccaacttg aacaccctgg tggatttccg ctacgcgcgc cggcacgagg ccaggcgcgg
     1381 ccagcacggc atgggctccg acatgttcgg cgccgccggc gacgacatca cgctgcgcat
     1441 gccggtgggc accatcatca gcgacgccga cacgggcgag gtgttgcacg agttgctgca
     1501 gccgggccag gcggtgctgg tggccaaggg tggcgacggc ggtttcggca acctgcattt
     1561 caagagctcg accaaccgcg ctccacgcca gaagacaccc ggctggccag gtgaaaaacg
     1621 cagcctgaag ctggagctga aggtgctggc cgacgtcggc ctgctgggca tgcccaatgc
     1681 cggcaagtcg accttcattg ccgccgtcag caacgcacgg ccgaagatcg ccgactaccc
     1741 gttcaccacc ctgtacccca accttggtgt ggtgcgcgtg gggccggagc agagcttcgt
     1801 ggtggccgac atcccgggcc tgatcgaagg tgccagcgaa ggcgccggcc tgggccatca
     1861 gttcctgcgc catctgcaac gcacgcgcct gctgctgcac atcgtcgaca tcgcgccgtt
     1921 cgacgagggc gtggatccgg tggtgcaggc gcgcgccatc gtggccgagc tgaagaagta
     1981 cgacccgtcg ctgcacgcca agccgcgctg gctggtgctc aacaagctcg acatggtgcc
     2041 gcccgccgaa cgcgaagccc gcgtgcgcga gttcgtgcgc cgcctgcgct ggaaggggcc
     2101 ggtgtttcag atctcggccc tcacgcgtga aggctgcgag cccttgatcc aggcggtgta
     2161 ccagttcgta cgtgcgcagc aggtggccga cgcaccggcc gagccacccg atccgcgctt
     2221 tgccgacgcc gctgaaccac atcaaaaatg atagctgttg gcgcttgcct ggcaagtgct
     2281 atcgcctcaa atgactcaga attcggtatc ggaggtgctg cgtcaggcgc agcgcatcgt
     2341 catcaaggtg ggctccagcc tggtcaccaa cgaggggcgc ggcctggacg agaccgccat
     2401 cggcgaatgg tgtcgccaga tggcgcggct ggccggccag gccgacctgc cgcgcgaagt
     2461 cgtcatggtg tccagcggcg ccatcgccga gggcatgaag cgcctgggct ggacccagcg
     2521 accgagcgag gtcaatgaac tgcaggccgc tgccgcggtc ggccagatgg ggctggcgca
     2581 gatgtatgaa accaagctgc gcgagcaggg cctgcgcagc gcccaggtgc tgctgacgca
     2641 cgccgacctg gccgaccgcg agcgctacct gaacgcccgc tcgacgctgc tgaccctgct
     2701 ggagcaccgt gtgctgccgg tcatcaacga gaacgacacg gtggtcaccg acgagatcaa
     2761 ggtcggcgac aacgacactc tgggcgccct ggtggccaac ctgatcgaag ccgacctgct
     2821 ggtcattctg accgaccaga agggcctgta caccgccgac ccgcgcgcca acccgcaggc
     2881 cgagttcgtg catgaggcgc gggccggcga tcctgccctg gaacgcatgg ccggtggcgc
     2941 cggctccagc atcggcaaag gcggcatgct gaccaagatc ctggcggcca agcgggcggc
     3001 gcgttcgggc gcctcgaccg tgatcgcctg ggggcgggag ccggacgtgc tgctgcgcct
     3061 gtcgcggggc gagcgcatcg gcaccctgct ggtggcgccg acccagaagc tgcaggcgcg
     3121 caagcagtgg atggccgacc atctgcagct gcggggggcg ctggtgatcg atgacggcgc
     3181 cgtggccaag ctgcgtggcg ccggcaagag cctgctgccg atcggcatga cgcaggtcga
     3241 gggcgcattc gcgcgtggcg acgtggtggc catccgcgac gcccgcggcg ccgaagtggc
     3301 gcgcgggctg gccaactact ccagcgccga ggcgcgcctg ctgtgccggc ggccgtcggg
     3361 cgatatcacg gtgctgctgg gctacatggc cgagccggag atggtgcacc gcgacaacat
     3421 ggtgctgatc cgctgagcgc ggccaggcgg tgacgttgca gcaccggtgt ggcgcggccg
     3481 cttagtctca gtagtagagc gagcggttgc cggtcttgag ctcgcgcacc atttccgaca
     3541 ccgtctgccg cgcgctgggg ccgaggcaaa ggccatcgcg ggccaggcgc agcgggtgct
     3601 gcaccagcac gctggactgg ccgcgcatgc ttttccagcc gctgtcggtg ctgggcgtcc
     3661 attcaccccc ttgcacctgg cgcgcgtaca cgcggaactc gccggtggtg caacggatgc
     3721 cttcgtagct ggcgttgacg gccgacgtgc cgcgcgccac caccacgtag cgcaccacgc
     3781 cgctctgatg gttgacgctc agggtctgcg ggtcgatgcc catgcgcacc gacgccgtct
     3841 tgggcatgtc gatctccagc agccgctcca ggctgtaggc cggtggcggc ggcaccgggt
     3901 cttcctgcca gtcggcccga tccaggtcgg catactgggc cgcgccgggc gcgctgaaca
     3961 ggccggcgca ggccgccagc aggcaggcca gccaggcgcg ccgccccggg ggacgcaggc
     4021 ggcggctcat ggcttgtccg gcgggccgaa aggcggacgg ggcacgccct ccagcgtcgc
     4081 gccagggggc agggcgatgg ccgccgaggc ggcacgggcc aggcgatcgc gggtctggat
     4141 ggcgggcagt tcgccgccgt cgcgggcacg cacgccgcca cgcaggtaac ggttgcggtt
     4201 ctcggtgcgc ggcagatagc gggccagctc ggtcagcgcc atttcgtaga cgccgcgctt
     4261 gaattccacc accacgtcca gcggcaccca gtaatcgttc cagcgccagg cgtcgaactc
     4321 ggggtggctg gtggcgcgca ggttcagctg ccagtcctgg gcgatcaact gcaacagata
     4381 ccagatctgc ttctggccct tgtagtggcc gcgcgcgtcg cggcggatga accggtcggg
     4441 cacctcgtag cgcaaccagt cgcgggttcg ggccacgatg cgcacatgtt ccggctgcag
     4501 ccccacttcc tcgtgcagtt cccggtacat ggcctgctcg ggcgattcgc cccggtcaat
     4561 gccgccttgc gggaattgcc aggaatgggt gcggatgcgc ttgccccaaa atacctggtt
     4621 cttctggttg agcaggatga tgccgacgtt cgggcgaaag ccttcccggt caagcataat
     4681 caaacctcaa aatctaaaaa ttgtgtccat tatgcacggc gcaggctgcc cggcaagtgg
     4741 gcacaaaccc gcttgcacgg cgccccgact gcgccagacc ccccatgaaa gcctcccagt
     4801 tctacatcag caccctgaaa gaagccccgg ccgacgccga aatcgtgagc caccagctca
     4861 tgacccgtgc cggcatgatc aagaagctgg gtgccggcat ctacacctac atgccgatgg
     4921 gcctgcgcgt gatccgcaag gtcgaggcca tcgtgcgcga agagatgaac cgcgccggcg
     4981 cggtcgagtg cgtgatgccg gtggttcagc cgggcgagct gtggagtgaa agtgggcgct
     5041 tccagaccat gggcccggag ctgatgcgca tccgggaccg ccacgagcgc gacttcgtgg
     5101 tgcagcccac cagcgaggag gcggtgaccg acatcgcccg ccaggacctg cgcagctaca
     5161 agcagctgcc gcgcaacctc taccagatcc agaccaagtt ccgcgacgag cgccggccgc
     5221 gcttcggcct gatgcgcggg cgcgaattca tcatgaagga cgcctacagc ttcgaccgcg
     5281 acgaaccggg cgccaaaagg agctatcagg ccatggcgcg cgcctaccgc gccatctttg
     5341 accgctttgg cctgcgctac cgcgcggtgg cggccgattc cggggccatc ggcggcgatc
     5401 tgagcgagga attccaggtc atcgccgcca ccggcgaaga cgccatcgtc tactgccccg
     5461 gcagcgacta cgccgccaac atggagaagg ccgaggcgct ggcgcccgcc ggcccgcgcc
     5521 cggcgcccgc gcaggccctg acgaacacgc ccacgcccgg caaggccacc tgcgccgaag
     5581 tggccgaact gctgggcctg ccgctggcgc gcaccgtcaa gtcgctggtg ctggccaccg
     5641 acgagctgga cgcgcaaggt gccgtggcca agacccagat ctggctgctg ctgctgcgcg
     5701 gcgaccacga catgaacgag atcaaggccg ccaaggtgcc ggggctgaat gcgggctttc
     5761 gctttgccac cgtgcccgag atccaggcgc actttggcac gccgcccggc tacctgggcc
     5821 cgatcggcct gaagcggccg gtcaaggtgg tggccgaccg cgaggtggcg ctgatggccg
     5881 actgggtgtg cggcgccaat gccgagggcc atcacctgac aggcgtgaac tgggggcgcg
     5941 acctgcccga gccggatgtg gtggccgacc tgcgcaatgt ggtcgccggc gatgcctcgc
     6001 ccgacgggcg cggcgtgctg gcgatcgagc gcggcatcga gatcggccac atcttctacc
     6061 tgggcaccaa gtacagccgc gccatgaacg ccaccttcct ggacgaggac ggcaagccca
     6121 agccgttcga gatgggttgc tacggcatcg gcatcacgcg cctgccggcg gccgccatcg
     6181 agcagaacca cgacgcgcgc ggcatcatct ggcccgatgc catcgccccc ttcacggtgg
     6241 tcatctgccc ggtcggcatg gaccgcagcg agtccgtcaa gaccgccgcc gaagccctgt
     6301 acgccgaact gctggccgcc ggggtggacg tgatcctgga cgatcgcggc gagcgaccgg
     6361 gtgtgatgtt tgccgactgg gagttgatcg gtgtgccgca ccgtgtcacc atcggtgaca
     6421 aaggcctcaa ggacggacag gtggaatacc agcatcgccg cgacagcacc gccactcggg
     6481 tgccggtgga cggcatcgcc gccttcgtga cgggccggct ggcggtgtga acgtcggggc
     6541 cgagcggtct ggaggtgcgt cgggcgtgga tcgtaggcag ctcatccgag gcttgatggc
     6601 cgtgccaggc gcgctcattt ttcaggagcg ggcgctggcc ggcggccagc tggaggagcc
     6661 gctggccgac gcggtgcgca cggcgctgtc ctcggccatc gccaacgatg cgccgccgct
     6721 gccggtgttt gcctccaccg acgcgcgcct gcgctacctg cgctggctgt cgggcatgag
     6781 cgaccgcctc tacagccgca agaaggactt caacgcccgc atcgaatttc tgcagacggt
     6841 ctggtatgag tcgcagcgcg ccgggctcga gacctcgctg gtgctggggc tgatccaggt
     6901 cgaaagcgcg tttcgcaaat acgcggtgtc cagcgtgggc gcgcgtggct acatgcaggt
     6961 gatgccgttc tggagccggg tgatcggtga cggcgacgcc ggcaagctgt ttcacatgca
     7021 gaccaacctg cgcttcggct gcgtgatcct gcgccactac ctggagcgtg agcgcggcga
     7081 cctgttcatg gcgctggggc gctacaacgg ctcgcgcggc cgaccgcagt atccgaacgc
     7141 cgtgttcggg gcccagaaaa gatgggtttt gtcggcctga cgcttgtctg gcttgcgcca
     7201 gcagctatca aaatagtagt cactccgggg ctgaacgctc actgcgggtg accagcacct
     7261 ggtcgatccg gtagttgtcc acgtccatca cctcgaagcg gtggccgccc cactgcacgc
     7321 tgtcggtgcg gcgcggcacg cggcgcagca tcaccatcag aaagccggcc agggtgtcgt
     7381 aggcctcggc gctgggcggc tcgtccaggt tcagcgcgcg ctgcacgtcg gccattggcg
     7441 tgtggccgtc gatcagccag gagccgtcgt cgcgcagcac gatcggctcc tcgtcctcgg
     7501 gcgtgaccag ctcgcccatc accgtgctca tgacgtcgtt gagcgtgacc acgccgacga
     7561 cggtgctgta ttcgttgacg atcagggcga aatcctcgtg ctgctggcgg aactggtgca
     7621 gcacctccga caggctcagg ctgtcgggca ccaccaccac cttgtgcagc agcgccggtt
     7681 cggtcagctc gatcgggcgc tggtggagca cgcgctgaaa caggtccttg gcgtccacgt
     7741 agcccagcac ccggtccagt tcgccctcgc acaccgggta ggtggaaaac ggctcggccg
     7801 ctatgcgcag gcggatttcg gcgtcgtcgg cgtcgtggcg cagccaggcg atgcggtcgc
     7861 gcggcgtcat ggcgctggtg acggtgcgcg tgtccagctc gaacacgttc tcgatcacct
     7921 gctgcccggg ccggtccagc acgccggcct gcgtgccggc ttcggccagc gccaggatgt
     7981 catccggcgt gatgcgctcg tcgcgctgca tgggcagacc cagcaggcgc atcaccaggt
     8041 cggtgcaggc ggtgtagagc cagacgatgg gcttgaacag cagcagcaca tgccccatcg
     8101 gccccaccat ggccatggcc acgcgttcgg gctcggccat gcccaggcgt ttgggcacca
     8161 ggtcggcaaa caccacgaac agcgaggtca ccgccgccac cgagccgacc agcgacaggt
     8221 tcgccgccgt ccgttcgggc aaccacagcc gcagcagctc ggccaggtac ggtcccagca
     8281 gaccttcacc cacgatgccg cccaggatgg cgacggcgtt caggccgatc tgcaccgtgg
     8341 tgaagtaaaa ccccggctgc tcctgcaccc gcagcacgcg cagcgcacgc gcatccccgt
     8401 cgtcggccag ctgacgcagg cgcagacgac gcgaggcggc cagggcgatc tcggccagcg
     8461 aaaaaaacgc gctgacggca atgagaagtg cgatcagcag caggctttgg gtcaggctca
     8521 tgaatccggg gtggccgcca atggcgcggc gggcacgctt ttgtcggccc ggcgggcacg
     8581 acgcgccgca gggcagtgta cacagcggcc accggccggc ggaagcaggc ggtcacaggc
     8641 cgtgggcggc ggcttcctat aatttttgcg atccaccacg ggccagacgc ccgcccgaaa
     8701 cgccggcgcg cttgcgtgcg gcccatccac acaagccacc attgaaggat ctccatgttc
     8761 gacgtctaca ttcgtgaagc cggtgcccgt tttggcctgg gcgagcaggc cctgaccgtg
     8821 gcccagatgc tgctggccga catgaccagc cccgacaccg gcgggttgat gggttttctg
     8881 gagcgtttca aatccgccgg cctggggccc gtcatccagt cctggctcgg cggtggcccg
     8941 agcgcacagc cgatcagcaa cagccagctg gaaaccgtgc tgggcgccag tggcgggctg
     9001 ctgtcgcgcc tgacggggct gctggacgcc ccgcgcgaca acgtcaccac cgcgatcggc
     9061 tacctgctgc ctgccttcgt cgggcgcctg acgcctgggg gcagcctgcc gtcgtcgctg
     9121 ccgcccgagg tgctcagtct ggcggcggca gggcgcgatc tgctgaccgc gcctgccgcg
     9181 gccagcgcac cggtggcggc gggccagccc ggcatgctcc ggtggctgcc ctgggtgatt
     9241 gtggcggccg cggcgttgat cggtctggcg tactgcggca agcaggagca ggcgaccccc
     9301 agcgcaccgg cgacgccggc cgtcgacgcg gcgagcacgg ccgcgcctgc cgccggggcg
     9361 tccgcggccg ccgacgcggc gtcaccggct gcgcccagcg ccagcccggc cgctgccgcg
     9421 gcctccgggc ccgagccggg cgccgctgtg gaggctctga ccgacgcggc gtcacgccct
     9481 gcgctgcagg tgttcttcga ttccggcaag gccgaggtgc accccgatct ggcggcgcgc
     9541 gccgccgatc tgctggccta cgtgaaggcc aaccaggggg tgcaggtcgt catcgctggc
     9601 ttcaacgacc cgaccggcga cgccgccgcc aacgccgagc tgtcgcgcca gcgcgcccag
     9661 gcggtgcagg ccgcgctggt ggcggccggt ctggcggccg agcgcacggt gctggagaag
     9721 ccggccgaca ccacggccac cacgtccagt ctggcggccg cgcgccgggt ggacgtgacg
     9781 ctgcgtccgt gacgccggca ggggcacaac aaaaacggac gccgcggcgt ccgtggtggt
     9841 gcggggcgcg gcggtgccga gtcagctctt gccgccgacc agctgctgca gttcgcccga
     9901 ctcgtacatt tccatcatga tgtccgagcc gccgatgaat tcaccgcgga cgtacagctg
     9961 cgggatggtg ggccactggc tgtattcctt gatgccctga cgcaccgcct cgtcatccag
    10021 cacgttgacg gtcctgacgg cgctggtgtc gacgccgcag gctttcagga tctgtacggc
    10081 gcggccggaa aagccgcaca tcgggaagtt cgcatcacct ttcatgaaca gcacgatgtc
    10141 gttgcccttg acgaggcctt cgatgtgctg ctgcgtggtc tgatccatgg ctggggttcc
    10201 ttcaatgtga tggttccgat tattccacct tcgacctgcc acagccgccg cagggggatc
    10261 gtgccaggcg cggcgcgcag cggcagcgtt ggcacggggc gcatctgcgt gtcgatgatg
    10321 cgtcggcccg cggcggtcgg tcggggtcat gaaaaaggcg ccctgatggg cgcctttgcc
    10381 tggtgcggtg caaccggttt cagccgatcc ggaacaggtt gcggtctttg ccgtcaatcc
    10441 acttgggcgg cttgccgcgg ccggtccagg tctggccggt ggccggatcg cggtatttgg
    10501 gggccacctt gccggccatt ctaggcgtcg ccgcgcggcg gccggcgggg aaaacatctt
    10561 tttcggtcag gtcatattca ttcacgatct gacgtacccg ggccacggcg tcggcaattt
    10621 cacgcttgcg tgcttcttcc acctggacgg ccagcgccgc ctgctgtttc aggagttcct
    10681 gataggttgc catgagattc tcgctttctg atgtatatgg gcggtcggaa tatatccgat
    10741 cggcaacccc gaatcaataa t
//
LOCUS       DAOQTZ010000088        10751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_6890_length_10751_cov_12.711107, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000088 DAOQTZ010000000
VERSION     DAOQTZ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10751
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6890_length_10751_cov_12.711107"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..738)
                     /locus_tag="PKN51_10355"
     CDS             complement(118..738)
                     /locus_tag="PKN51_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT85544.1"
                     /translation="MRRAVEQVVNGSSHSLFLRALVAQPARVGAICASSPQLAARMAA
                     WVDPDAPGLVVELGGGTGVITAALLARGVAPQRLVVIEQSPVLARHLAQRFPQVRVVQ
                     GDAAELALLQRAPDWPRGDDGAPRPVHCIVSGLPLLSIPPQLRQRILQAGAQTLAPGG
                     RLLQFTYALRGPSPWQVTGLSGCARERVLANLPPARIDVLQRPHLG"
     gene            complement(900..2210)
                     /locus_tag="PKN51_10360"
     CDS             complement(900..2210)
                     /locus_tag="PKN51_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85545.1"
                     /translation="MSLVPSLVRHVLLLAALGGGVAGVASAQAARAPRAQLWIDLSTG
                     GGMAGMPEMELGAAGGLMGMLGSSAPAGMGGPPHYGMARGAAVMPPRIVDIALHNSLR
                     PGVEARQAIPPGMRMGDSLPLLPPRAEPRAPSEPGDMPEEYTRQPPRGRLLIYWGCGP
                     EVRAGQPRVIDLAQAGAAQLAQAFAGRVVPERGARVGPGHVLYPNERSQAAVPRGSSL
                     VGEHQVLGEGVPASMKFTLGSAQDLMPPIELSPSGGVQDSIATQWRAVPHARAYYLHA
                     MASAGDDMILWSSAETPDTGMGLFDYLPNATIERWVRERVLLGADVTQCAIPRGIFAA
                     GGSDATPMLRMMAFGGESHIVHPPRPADPKARWEPEWAVRVRVKAHTMAMLGDSGAAM
                     RGARPGGGASAGATGQGAEPRPDDGSPAQILLNPGNLLRGIFGR"
     gene            complement(2315..3292)
                     /locus_tag="PKN51_10365"
     CDS             complement(2315..3292)
                     /locus_tag="PKN51_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNT85546.1"
                     /translation="MVTRKPKGLGRGLDALLGPSPNGDGAEGAGAATPAGTPMMLALE
                     QLAPGQYQPRTRMDEGALYELAESIKSQGIMQPILVRRIAGPKPASGLDGQAPAATFS
                     GASYEIIAGERRFRAARLAGLTEVPVLLRDVPDEAAAAMALIENIQREDLNPLEEAQG
                     IRRLVEEFGLTHEQAAQAVGKSRSAASNLLRLLQLSEPVQTMLMAGDLDMGHARALLA
                     LEGAAQITAAHQVAARQLSVRETEALARKLSAEFNLTAPRQRARAPDKSGDVRRVEEE
                     LSDLLLAQVEVRVRKSGRRGGAAAQAGEVAIQFASLDELNGLIERLRGG"
     gene            complement(3331..4767)
                     /gene="aspA"
                     /locus_tag="PKN51_10370"
     CDS             complement(3331..4767)
                     /gene="aspA"
                     /locus_tag="PKN51_10370"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNT85547.1"
                     /translation="MSTPNPNPNRIEHDLIGERELPNDVYYGVHTLRALENFPITGTA
                     ISIYPDLVRALASIKKAAALANRDLGLLSEAHCQAIGAACDELLAGRLHEQFVVDVIQ
                     GGAGTSTNMNANEVIANRALELMGHAKGEFQHLHPNEHVNLGQSTNDVYPSALKIAAW
                     HGISDLIASMAYLRAAFEAKAEEFKDVLKMGRTQLQDAVPMTLGQEFGTYAVMLGEDE
                     QRLREAQQLICELNMGATAIGTGITAHPDYAAKVIAHLRAITGIPLATAPNLIEATQD
                     CGAFVQLSGVLKRVATKLSKTCHDLRLLSSGPRAGLGEINLPAVQAGSSIMPGKVNPV
                     IPEVVNQIAYEVIGNDVTVTFAAEAGQLQLNAFEPVIAHSLFKSVKHLSAGCRTLADR
                     CVRGISANVQRMHDTVTHSIGIVTALNPYIGYAHATEVAQAAHASGGSVYDEVLKRGL
                     MTREQLDAVLRPEILTRPVPLDLAPKRS"
     gene            complement(4778..5367)
                     /locus_tag="PKN51_10375"
                     /pseudo
     CDS             complement(4778..5367)
                     /locus_tag="PKN51_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429996.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(5354..6484)
                     /locus_tag="PKN51_10380"
     CDS             complement(5354..6484)
                     /locus_tag="PKN51_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNT85548.1"
                     /translation="MKSLTEPRTPPAAGLPEPWIDWGKAIASQLIVWHHLAHYGPLAP
                     RVAAVWPDLMGWLDGRALYAVQAFLVMGGYLAARSLWPRPGAPRVAAADWPARAARRW
                     RRLMPTYLAALLLAVLCAALARQWMDDPDTPAAPTAGQLLAHLLLLQDLLEQPALSAG
                     LWYVAIDLQLYALLAALALLVGRAPARWRRASALALVVGGGAASVLVFNRQPGGDELA
                     PYFFGSYALGVLAAWGRAAGTAAGRRAAGALLLGLAGAGLALAWRDRLALAALCAMLL
                     LWQPGRTRLALAGQPWLRSVVAQLAAISYAVFLLHYPISLLVTALLIDALPAGPVPAA
                     ALLVLTWLLSLATGWALTQALQAPGLWQRLRRLTARWRHGTR"
     gene            complement(6529..7314)
                     /locus_tag="PKN51_10385"
     CDS             complement(6529..7314)
                     /locus_tag="PKN51_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT85549.1"
                     /translation="MAHIFCIANQKGGVGKTTTTVNLAAGLARIGQRVLLVDLDPQGN
                     ATMGSGVDKRAMALSVYDVLLESCSIAEAASVPERLKEAGAGYAVLGANRELAGAEVE
                     LVGEERRERRLKQALTAVDADYDFVLIDCPPSLSMLTLNGLCAAQGVIVPMQCEYFAL
                     EGLADLTNTIRQVHANLNRDLRLIGLLRVMFDARITLQQQVSDQLKAHYGSKVFDTII
                     PRNVRLAEAPSYGLPGVVFDPAAKGSQAFVAFAEELVRRGLSA"
     gene            complement(7321..7935)
                     /locus_tag="PKN51_10390"
     CDS             complement(7321..7935)
                     /locus_tag="PKN51_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNT85550.1"
                     /translation="MFGISDYGAFVAAIVLFLAVPGPGNLALLSSTAQGGVRGGLAAT
                     LGLIAGDQVLLWCAVAGVAALLQSAPTLFAAVQWLGAAYLAWLGWRMLSARPGQGAVL
                     RLAPRHFLRQAFLITLLNPKAIVFYMAFFPLFVDPARHQGLLSFGAMALTIAVLTFLY
                     GLGATLIAHHMAARIRAHPRLSGGLQKLAGVFLIGFGLRLALTK"
     gene            complement(7964..8605)
                     /gene="rsmG"
                     /locus_tag="PKN51_10395"
     CDS             complement(7964..8605)
                     /gene="rsmG"
                     /locus_tag="PKN51_10395"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNT85551.1"
                     /translation="MHPQRAALGAGAQALGLALDERQIDRLLAYLDLLLKWNRVYNLT
                     ALRDPADMLTHHVLDSLALIAPLRAHAGGRALRLLDVGSGGGLPGVVIAIGCPGIDVT
                     CVDAVGKKAAFVQQVAGALGLANLHGQHGRVEAITGRFDVVTSRAFASLADFTAWSDG
                     ALAEGGVWLAMKGRRPDDEIAALPPAVTVFHVEPLTVPGLEAERCLVWLRRAG"
     gene            complement(8605..10581)
                     /gene="mnmG"
                     /locus_tag="PKN51_10400"
     CDS             complement(8605..10581)
                     /gene="mnmG"
                     /locus_tag="PKN51_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNT85552.1"
                     /translation="MPTPSFFHPQTFDVIVVGGGHAGTEAALAAARMGAQTLLLTHNI
                     ETLGQMSCNPSIGGIGKGHLVKEVDALGGAMALATDEAGIQFRILNSSKGPAVRATRA
                     QADRVRYKAAIRRRLENQPNLWLFQQAVDDLLLQGDRVAGAVTQLGIAFRARAVVLTA
                     GTFLDGRIHVGLANHAGGRAGDPPALSLSARLKELKLPQGRLKTGTPPRLDGRTIDFS
                     QCELQPGDGMPGGQNDRMPVFSFMGRADMHPPQMPCWVTHTNARTHEIIKSGFDRSPM
                     FTGVIEGVGPRYCPSVEDKVHRFAGKDSHQIFLEPEGLDTHEVYPNGISTSLPFDVQL
                     ALVRSMRGLENAHILRPGYAIEYDYFDPRALRSSFETRAIGGLFFAGQINGTTGYEEA
                     AAQGLFAGINAALQCRGEAPWLPRRDEAYLGVLVDDLVTQGVTEPYRMFTSRAEFRLQ
                     LREDNADMRLTDTGRRLGLIDDARWGAFCRKRDAVSRETERLKSTWVNPRQLPASESE
                     RVLGKAIEHEHNLFDLLRRPGVSHAALVGLEGGRYASAEVSRETLGELWAPVIEQVEI
                     AAKYAGYIDRQREEVERAAHFEHLRLPPDLDYMQVPALSIEVRQKLNLHRPETLGQAS
                     RISGVTPAAISLLLIHLKKGGFKGFAGGGAAEAA"
BASE COUNT         1518 a         3840 c         3875 g         1518 t
ORIGIN      
        1 gcctggcacg ccgatcacgg cacgggcggg cgcgtccaac tgccgtttct aggtttaata
       61 gctattggcg ctttatgggc gggccccaga cccagaaatg gtgccggatc agtgccctca
      121 gccgaggtgc ggtcgttgca gcacgtcgat ccgcgcaggc ggcaggttgg ccagcactcg
      181 ctcacgcgca cagcccgaca ggcccgtcac ctgccagggc gaaggccccc gcagcgcata
      241 ggtgaactgc agcaggcgcc cgcccggcgc cagcgtctgc gccccggcct gcaggatgcg
      301 ctgacgcagc tgcggcggaa tcgacagcag cggcaggccg gacacgatgc agtgcaccgg
      361 ccggggcgcg ccatcgtcgc cgcgcggcca gtcgggtgcg cgctgcagca gggccagctc
      421 ggcggcatcg ccctgcacca cccgcacctg cgggaagcgc tgcgccaggt gccgcgccag
      481 caccggcgac tgttcgatca ccaccagacg ttgaggcgcc acaccgcgcg ccagcagggc
      541 ggcggtgatg acgccggtgc caccgcccag ctcgaccacc aggcccggcg cgtcgggatc
      601 gacccaggcc gccatgcgtg ccgccagctg gggcgagctg gcgcagatgg cccccacccg
      661 ggccggctgc gccaccaggg cgcgcaggaa cagcgagtgc gacgagccgt tgacgacctg
      721 ctctacggcc cggcgcatgc gcagtacgcc ggcgcgcgcg gcccgcaggg tcagcagcgg
      781 tgccgccctg gccggaccac ggcggcgcgc cagccggacc acggcgcgca cgccagggcg
      841 ctgcggcacg tcggcagcgc cggacgaaga aggatcggac acgctcatgg ttcgaccgtt
      901 caacgcccga agatgccgcg cagcaggttg cccgggttga gcaggatctg cgccggcgag
      961 ccgtcgtccg gccggggctc ggcgccttgc ccggtggcac cggcgctggc gccgccgccc
     1021 ggccgggcac cgcgcatggc ggcaccgctg tcgcccagca tggccatggt gtgggccttg
     1081 acgcgcacgc gcaccgccca ttcgggctcc cagcgcgcct tcgggtcggc cggacggggc
     1141 gggtgcacga tgtggctctc gcccccgaag gccatcatgc gcagcatggg ggtggcgtcg
     1201 ctgccgccgg cggcgaagat gccgcgcgga atggcgcact gggtgacgtc ggcccccagc
     1261 agcacgcgct cacgcaccca gcgctcgatg gtggcgttgg gcaggtagtc gaacaggccc
     1321 atgccggtgt ccggcgtttc ggcgctggac cacaggatca tgtcgtcgcc ggccgacgcc
     1381 atggcgtgca gatagtaggc gcgcgcatgg ggcacggcgc gccattgggt ggcgatgctg
     1441 tcctgcacgc cgccgctggg gctgagctcg atcggcggca tcaggtcctg ggcgctgccc
     1501 agagtgaact tcatcgacgc cggcacccct tcgcccagca cctggtgttc gcccaccagc
     1561 gagctgccgc gcggcaccgc cgcctggctg cgttcgttgg gatacagcac atggcccggg
     1621 ccgacgcgcg cgccgcgttc gggcaccacg cgcccggcaa aggcctgcgc cagctgtgcc
     1681 gcgccggcct gggccagatc gatcacgcgc ggctggccgg cgcgcacctc gggcccgcaa
     1741 ccccagtaga tcagcaggcg cccgcgcggc ggctggcgcg tgtactcctc gggcatgtcg
     1801 cccggctcgg agggcgcgcg gggttcggca cgcggcggca gcagcggcag gctgtcgccc
     1861 atgcgcatgc cgggcgggat ggcctggcgt gcctcgacac cggggcgcag gctgttgtgc
     1921 agcgcgatgt cgacgatgcg cggcggcatc accgccgcac cgcgcgccat gccgtagtgc
     1981 ggcggcccgc ccatgccggc gggtgcgctg ctgcccagca tgcccatcag gccgccggcc
     2041 gcgcccagct ccatctcggg catgccggcc atgccgccgc cggtggacag gtcgatccac
     2101 agctgcgccc tgggcgcgcg cgcggcctgc gccgaggcga cgcccgccac acccccaccc
     2161 agggcggcca gcagcagcac atgtcggacg agcgatggca ccaggctcat gatgggattc
     2221 ctgcgttcgt ttcggtgatg tggcgcacac gccgtgcgga cgatggttca gtgtatgccg
     2281 atcgggcgcc gtgcagcgca ttcgggcccc tgcatcatcc gccgcgcagc cgctcgatca
     2341 gcccgttgag ctcgtccagc gacgcgaact ggatcgccac ttcgcccgcc tgggccgccg
     2401 cgccgccgcg ccggccgctt tttctgaccc gcacctcgac ctgcgccagc agcaggtcgg
     2461 acagctcctc ctccacccgc cgcacgtcgc ccgacttgtc gggcgcgcgc gcgcgctggc
     2521 gcggcgccgt caggttgaat tcggccgaca gcttgcgcgc cagcgcctcg gtttcgcgca
     2581 cgctgagctg gcgcgccgcc acctggtggg ccgcggtgat ctgcgccgcg ccttccagcg
     2641 ccagcagcgc gcgcgcgtgg cccatgtcca ggtcgccggc catcagcatg gtctgcaccg
     2701 gttcggaaag ctgcagcagg cgcagcaggt tgctggcggc gctgcggctc ttgccgaccg
     2761 cctgtgcggc ctgctcgtgg gtgaggccga actcctcgac caggcgccgg atgccctggg
     2821 cttcttccag cgggttgagg tcttcgcgct gaatgttttc gatcagcgcc atggcggcgg
     2881 cggcctcgtc gggcacgtcg cgcaacagca ccggcacctc ggtcaggccc gccagacgcg
     2941 ccgcgcgaaa gcgccgctcg ccggcgatga tttcgtacga tgctcccgaa aatgtagctg
     3001 ctggcgcttg tccatcaagc cccgaggccg gttttggccc ggcaatgcgg cgcaccagga
     3061 tgggctgcat gatgccctgg ctcttgatgc tttcggccag ctcgtagagc gcgccttcgt
     3121 ccatgcgcgt gcgcggctgg tactggccgg gagcgagctg ctccagcgcc agcatcatcg
     3181 gcgtgccggc cggggtggcg gcgccggcgc cctcggcgcc gtcgccgttc gggctggggc
     3241 ccagcagcgc gtccaggccg cggccgaggc ccttgggttt tctggtgacc atggtcgatc
     3301 gatctcccgt acggtgcgcg gtcgaccggt tcacgaccgc ttgggcgcca ggtcgagcgg
     3361 caccgggcgg gtcaggattt cgggccgcag cacggcgtcg agctgctcgc gcgtcatcag
     3421 gccgcgcttg aggacttcgt cgtacacgct gccgccgctg gcatgtgcgg cctgcgccac
     3481 ttcggtggcg tgggcgtagc cgatgtaggg gttgagcgcg gtgacgatgc cgatcgagtg
     3541 cgtcaccgtg tcgtgcatgc gctgcacgtt ggcgctgatg ccgcgcacgc agcggtcggc
     3601 cagcgtgcgg cagccggccg acaggtgttt gacgctcttg aacaggctgt gcgcgatcac
     3661 cggctcgaag gcgttgagct gcagctgccc ggcctcggcg gcgaaggtga ccgtcacgtc
     3721 gttaccgatc acctcgtagg cgatctggtt gaccacttcg gggatcaccg ggttgacctt
     3781 gcccggcatg atggacgagc ccgcttgcac cgccggcagg ttgatctcgc ccaggccggc
     3841 gcgcgggccg ctggacagca ggcgcaggtc gtggcaggtc ttggacagct tggtggccac
     3901 gcgcttcaag acgcccgaca gctgcacgaa agcgccgcag tcctgcgtcg cctcgatcag
     3961 gttgggcgcg gtggccagcg ggatgccggt gatggcgcgc aggtgcgcga tcaccttggc
     4021 ggcatagtcg gggtgggcgg tgatgccggt gccgatggcc gtcgccccca tgttcagttc
     4081 acagatcagt tgctgcgcct cgcgcaggcg ctgctcgtcc tcgcccagca tgacggcgta
     4141 ggtgccgaac tcctgcccca gcgtcatcgg caccgcgtcc tgcagctggg tgcggcccat
     4201 cttgagcacg tccttgaatt cctcggcctt ggcctcgaag gcggcgcgca ggtaggccat
     4261 ggatgcgatc aggtcgctga tgccgtgcca ggcggcgatc ttgagcgcgc tggggtagac
     4321 gtcgttggtg ctctggccca ggttgacgtg ctcgttgggg tgcaggtgct ggaactcgcc
     4381 cttggcgtgg cccatcagct cgagcgcgcg gttggcgatg acctcgttgg cgttcatgtt
     4441 ggtcgaggtg ccggcgccgc cctggatcac gtcgaccacg aactgctcgt gcagccggcc
     4501 ggccagcagc tcgtcgcagg cggcaccgat ggcctggcag tgcgcctcgc tcagcagccc
     4561 gaggtcgcgg ttggccagcg ccgcggcctt cttgatgctg gccagcgcgc gaaccaggtc
     4621 ggggtagatc gagatcgccg tgcccgtgat cggaaagttc tccagcgcgc gcagggtgtg
     4681 cacgccgtag tagacgtcgt tgggcagctc gcgctcgccg atcaggtcgt gctcgatgcg
     4741 gttggggttg gggttggggg tgctcatggg cgccagtcta agcggttgac ccgcccgggg
     4801 cacaggtggg cgatggcaac aaggcgtaca gcagcgccag gccctgcgcc cagtcaccca
     4861 ggccggaatc ggcgttgagg tggccgcgcg cgcccagatc gcggaaggcg gcgccccagt
     4921 cctgcgccag cgccgggcat gggccagtcg gccaaacggg tcgtcgctgc tggccaccac
     4981 caggctggca aagggcaagc gctggcgcgc cggcggcgcc cagccgggga tctgctggcg
     5041 caggtcgtcg cgctcgatct cgggcggcgc caccagcagc gcgccgctca cgcgcgtggc
     5101 gtggcgcgaa tgcgcggccc accaggccac cagcaggcag cccaggctgt gcgcggccag
     5161 cagcagcggc gtgccggccg gctgcgccag caccacctct tccagccgcg ccgaccagtc
     5221 gccgcgccgc gggtgctgcc agtcgtgctg ctcgacgcgc tggcagccgt ggcgctgctc
     5281 ccagaggctt tgccagtgcg ccggcccgct gcccagccag cccggcaggg tcagcacggt
     5341 ggcggcgggt gcgtcagcgt gtgccatggc gccaccgcgc ggtgagacgg cgcagccgct
     5401 gccacaggcc gggggcttgc agcgcctgcg tcagcgccca gccggtcgcc aggctgagca
     5461 gccaggtcag caccagcagc gcggcggccg gcaccgggcc ggccggcagc gcgtcgatca
     5521 gcagggcggt gaccagcagg ctgatcgggt agtgcagcaa aaacacggcg taggagatgg
     5581 ccgccagctg cgccacgacg ctgcgcagcc agggctggcc ggccagtgcc agacgcgtgc
     5641 gcccgggctg ccacagcagc agcatggcgc agagcgcggc cagcgccagg cggtcgcgcc
     5701 aggccagcgc caggccggcg ccggccaacc ccagcagcag cgccccggcg gcgcggcgcc
     5761 ccgccgccgt gccggccgca cggccccagg cggccagcac gcccagggcg tagctgccaa
     5821 agaagtacgg cgccagctcg tcgccgccgg gctggcggtt gaacaccagc accgaggccg
     5881 cgccgccgcc gaccaccagg gccagcgccg aggcgcggcg ccagcgcgcc ggggcccggc
     5941 cgaccagcag cgccagcgcc gccagcaggg cgtacagctg cagatcgatg gccacatacc
     6001 acaggccggc gctcagggcc ggctgctcca gcaggtcctg cagcagcagc agatgcgcca
     6061 gcaactgccc cgccgtgggg gcggccgggg tatcggggtc gtccatccac tggcgcgcca
     6121 gcgccgcgca cagcacggcc agcagcagcg ccgccaggta ggtcggcatc aggcgccgcc
     6181 agcgccgggc cgcgcgggcc ggccagtcgg ccgccgccac gcgcggcgcg ccgggccgtg
     6241 gccacaggct gcgcgcggcc agatagccgc ccatgaccag aaacgcctgc accgcgtaca
     6301 gggcgcgccc gtccagccag cccatcaggt ccggccagac ggcggccacg cgcggcgcca
     6361 gcggaccgta gtgcgccagg tggtgccaga cgatgagctg cgaggcgatg gccttgcccc
     6421 agtcgatcca gggttcgggc aggccggcgg ccggcggcgt gcggggttca gtcagtgact
     6481 tcaatttcta tagctgccgg cggaatctgg acgggcgcca gaggcctttc aggcactcaa
     6541 accacggcgc accagctctt ccgcgaaggc gacgaacgcc tggctgccct tggccgccgg
     6601 gtcgaacacc acgccaggca ggccgtagct gggcgcctcg gccaggcgca cgttgcgcgg
     6661 gatgatggtg tcgaacacct tgctgccgta atgcgccttg agctggtcgc tgacctgctg
     6721 ctgcagcgtg atgcgggcgt cgaacatgac acgcagcaga ccgatcaggc gcaggtcgcg
     6781 gttcaggttg gcgtgcacct ggcggatggt gttggtcagg tcggccaggc cttcgagcgc
     6841 aaagtactcg cactgcatgg gcacgatcac gccctgggcg gcgcacaggc cgttcagtgt
     6901 cagcatgctc aaggacggcg ggcaatcgat cagcacgaag tcgtaatcgg catcgaccgc
     6961 cgtcagcgcc tgcttcaggc ggcgctcgcg gcgctcctcg cccaccagct cgacctcggc
     7021 gccggccagt tcgcggttgg cgcccagcac ggcgtagccg gcaccggcct ccttcaggcg
     7081 ctcgggcacg ctggcggctt cggcgatgct gcaggattcc agcagcacgt cgtacaccga
     7141 cagcgccatg gcccgtttgt ccacgcccga ccccatggtg gcattgccct gcgggtccag
     7201 gtcgaccagc agcacgcgct ggccgatcct ggccagcccg gcggccaggt tgacggtggt
     7261 ggtggtcttg ccaacgccac ccttctggtt ggcaatgcag aagatgtggg ccatgaggtc
     7321 ttacttggtg agcgccaggc gcaggccgaa gccgatcagg aacaccccgg ccagcttctg
     7381 cagcccgccc gacaggcgcg ggtgggcgcg gatgcgcgcc gccatgtggt gagcgatcag
     7441 cgtggccccg aggccgtaca ggaaggtcag cacggcgatc gtcagtgcca tggcgccgaa
     7501 gctcagcagc ccctggtgcc gcgccggatc gacgaacagc ggaaagaagg ccatgtagaa
     7561 cacgatggcc ttggggttga gcaaggtgat cagaaacgcc tggcgcagaa agtggcgtgg
     7621 cgccaggcgc agcaccgcgc cctgccccgg ccgggcgctc agcatgcgcc agcccagcca
     7681 ggccaggtag gccgcgccca gccactgcac ggcggcgaac agggtcggcg cgctctgcag
     7741 cagcgccgcg acgccggcga cggcgcacca cagcagcacc tggtcgccgg caatcaaccc
     7801 cagcgtggcc gccagcccgc cgcggacgcc accctgcgcc gtgctggaca gcagggccag
     7861 gttgcccggc ccggggacgg ccagaaacag cacgatggcg gccacaaagg caccgtaatc
     7921 ggaaatgccg aacatgggaa tgatcctgga gtgcgccgcg agtttacccg gcgcggcgca
     7981 gccagacgag gcagcgctca gcctccaggc cgggaaccgt gagcggttcc acgtgaaaca
     8041 cggtcaccgc gggcggcaag gcggcgattt cgtcgtccgg ccggcggccc ttcatggcca
     8101 gccacacccc gccctcggcc agcgcgccgt ccgaccaggc ggtgaaatcg gccagcgagg
     8161 cgaaggcgcg cgacgtcacc acatcgaatc ggcccgtgat ggcctcgacc cgcccgtgct
     8221 gcccgtgcag gttggccagc cccagcgcgc ccgccacctg ctgcacgaag gcggccttct
     8281 tgcccacggc atcgacacag gtgacgtcga tgccggggca gccgatggca atcaccaccc
     8341 ccggcaggcc gccgccgctg cccacgtcga gcaggcgcag cgcccgcccg ccggcgtgcg
     8401 cccgcagcgg cgcgatcagc gccaggctgt ccagcacatg atgggtcagc atgtcggcgg
     8461 ggtcgcgcag cgccgtcagg ttgtagaccc ggttccactt gagcagcaga tcgaggtagg
     8521 ccagcaggcg atcgatctgc cgctcatcca gcgccaggcc gagcgcctgc gcgccggcgc
     8581 cgagcgccgc acgttgcggg tgcatcaggc ggcctcggcc gcgccgccgc cggcaaagcc
     8641 cttgaaaccg ccctttttca ggtggatcag cagcagcgaa atggcggccg gcgtcacccc
     8701 gctgatgcgg ctggcctgac ccagggtctc gggccggtgc aggttgagct tctggcgcac
     8761 ttcgatcgac agcgccggca cctgcatgta gtccagatcg ggcggcaggc gcaggtgctc
     8821 gaaatgggca gcgcgctcga cctcctcgcg ctggcggtcg atgtagccgg cgtacttggc
     8881 ggcaatttcc acctgctcga tcaccggcgc ccacagctcg cccaaggttt cacgtgaaac
     8941 ctcggcgctg gcgtagcggc cgccttccag ccccaccagg gccgcgtggc tgacgcccgg
     9001 tcggcgcagc agatcgaaca ggttgtgttc gtgttcgatg gccttgccca gcacgcgctc
     9061 gctctcgctg gcgggcagtt ggcgcgggtt gacccaggtg gacttcagcc gttcggtttc
     9121 acgtgaaacc gcgtcgcgct tgcggcagaa ggcgccccag cgcgcgtcgt cgatcaggcc
     9181 cagccggcgg ccggtgtcgg tcaggcgcat gtcggcgttg tcctcgcgca gttgcaggcg
     9241 gaattcggcg cggctggtga acatgcggta aggctcggtc acgccctgcg tcaccagatc
     9301 gtccaccagc acgcccaggt aggcctcgtc gcggcgcggc agccagggcg cctcgccccg
     9361 gcactgcagg gcggcgttga tgccggcaaa cagtccctgc gccgccgcct cctcgtagcc
     9421 ggtggtgccg ttgatctggc cggcgaagaa caggccgccg atggcgcgcg tctcgaagct
     9481 gctgcgcagc gcgcgcggat cgaaatagtc gtattcgatg gcgtagccgg gacgcaggat
     9541 gtgggcgttt tccagcccgc gcatgctgcg caccagcgcc agctgcacgt cgaacggcag
     9601 gctggtggag atgccgttgg ggtagacctc gtgcgtatcc agcccttccg gctccaggaa
     9661 gatctggtgg ctgtccttgc cggcaaagcg gtggaccttg tcctccacgc tggggcagta
     9721 gcgcggcccc accccttcga tgacgccggt gaacatcggg ctgcggtcga agccgctctt
     9781 gatgatctcg tgcgtgcgcg cattggtgtg cgtgacccag cacggcatct gcggcgggtg
     9841 catgtcggcg cggcccatga agctgaacac cggcatgcgg tcgttctgcc cgcccggcat
     9901 gccgtcgccg ggttgcagct cgcactggct gaagtcgatc gtgcgcccgt ccagccgcgg
     9961 cggcgtgccg gtcttcaggc gcccctgcgg cagcttgagc tccttcagcc gcgccgacag
    10021 gctgagcgcc ggcgggtcgc ccgcccgccc gccggcgtgg ttggccagcc cgacgtggat
    10081 gcgcccgtcc aggaacgtac ccgccgtcag caccacggcg cgcgcacgga aggcaatgcc
    10141 cagctgggtg accgcgccgg ccacccgatc gccctgcagc agcaggtcgt ccaccgcctg
    10201 ctggaacagc cacaggttgg gctggttttc cagccggcgc cggatcgccg ccttgtagcg
    10261 cacacggtcg gcctgcgccc gcgtggcgcg caccgccggg cccttgctgc tgttgaggat
    10321 gcgaaactga atgccggcct cgtcggtggc cagcgccatg gcgccgccca gcgcgtcgac
    10381 ctccttgacc agatggccct tgccgatgcc gccgatgctg gggttgcagc tcatctgccc
    10441 cagggtttcg atgttgtgcg tcagcagcag ggtctgcgcg cccatgcgcg ccgccgccag
    10501 cgccgcctcg gtgccggcat ggccgccgcc gacgacgatg acgtcgaagg tctgggggtg
    10561 gaagaagctc ggggtgggca cgtcagaaaa accgtcaaca aagccccgga ttgtaggcat
    10621 tttctggcct cgccgcttgc cggatcagcg ccagcagcta tcattttcat agtcttctgg
    10681 cccggcgcgc gacggtcggg gcggccagcg agggcgcaac gcccgcctcc gtgttggtgg
    10741 ggcggttccc c
//
LOCUS       DAOQTZ010000089        10587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_7024_length_10587_cov_12.533422, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000089 DAOQTZ010000000
VERSION     DAOQTZ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10587
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7024_length_10587_cov_12.533422"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1484
                     /gene="tolB"
                     /locus_tag="PKN51_10405"
     CDS             183..1484
                     /gene="tolB"
                     /locus_tag="PKN51_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNT85553.1"
                     /translation="MKSALLDKFSLAPWQRRGFVTALAAAPFSAALAQFRVEVSGVGL
                     TQLPIALVPFRGEDAAPQKISAIVRADLERSGQFRAVDAGGQSMDETTRPEMSLWRQR
                     TADALAGGSVTRLADGRWDVRFRLWDVVRGQDLGGLGYVVPQADLRLAAHKIADYIYE
                     KLTGDKGVFSTRLAYVTKGGSRYTLWVADSDGENAQAALGSPEPIISPVWSPDGGQLA
                     YVSFESRKPVIYVHDVASGRRRLLANFKGSNSAPAWSPDGRSLAVTLSRDGGSQLYLI
                     SASGGEPRRLTQSQSIDTEPAFSPDGASIYFVSDRGGAPQIYRMPVGGGAAQRVTFSG
                     SYNVSPAISPDGRYLAYVSRTNGYRLHVMELASGTTTAVTDTAEDESPSFAPNSRLIV
                     YATRQGGREALMTTTLDGKIKARLAGQGGDIREPAWGPYQR"
     gene            1544..2083
                     /gene="pal"
                     /locus_tag="PKN51_10410"
     CDS             1544..2083
                     /gene="pal"
                     /locus_tag="PKN51_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNT85554.1"
                     /translation="MFKRYLMVAALAAVLAGCGSNVKLDPPIEGSASAGASAAGQGGA
                     SGQGAGQSTVTQVQAGAAGADAAGPNGVGRVIYFDFDSFVVKSEYQSLVDQHARFLQA
                     NRGRSVALEGHTDERGSREYNLALGQKRAEAVRRALTLVGASDAQVEAVSFGEEKPAA
                     AGASEDAYAKNRRVEIRYR"
     gene            2091..2888
                     /gene="ybgF"
                     /locus_tag="PKN51_10415"
     CDS             2091..2888
                     /gene="ybgF"
                     /locus_tag="PKN51_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNT85555.1"
                     /translation="MAIFRVTSLRAAVLALACLAGQGAQAQLFGDDQARQAILDLRQR
                     FDQAVAAQNRLVDENAQLRRGMLDLQQQIETLRAELARARGQDEQLARDLADLQKAQS
                     TQQQGWDERLRKLEQEASARMSDPNAAAGAPPVASDGAAASEKQDYDAALAVFRAGDF
                     KGAQTGFANFIKRYPRSTLAPSALFWLGNAQYATRDYKEAITNFRSLLTAAPSHARAP
                     EAMLAIANCQIELKDTRAARGTLESLIKTYPQSEAAQAGKDRLARLK"
     gene            3042..3404
                     /gene="rpsF"
                     /locus_tag="PKN51_10420"
     CDS             3042..3404
                     /gene="rpsF"
                     /locus_tag="PKN51_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNT85556.1"
                     /translation="MRHYEIILLIHPDQSEQVPAMLERYKGMITAGGGKIHREEDWGR
                     RQLAYQINKLAKAHYLCLNIEADQAVLGELEHAFRFNDAVLRHLTVQKKKAETAPSSM
                     MKTVEREEARKAQQAEAN"
     gene            3436..3738
                     /gene="priB"
                     /locus_tag="PKN51_10425"
     CDS             3436..3738
                     /gene="priB"
                     /locus_tag="PKN51_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HNT85557.1"
                     /translation="MNQLRLQARIVEIGALRHTPAGLPALDLRLEHESTQQEAGQSRQ
                     VRAALRAVALGAMAERLGRQVIGSQACFSGFLASPQRTRSVVFHIQDFQQDQFQGA"
     gene            3742..4023
                     /gene="rpsR"
                     /locus_tag="PKN51_10430"
     CDS             3742..4023
                     /gene="rpsR"
                     /locus_tag="PKN51_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNT85558.1"
                     /translation="MATFKKFNKDKRPKRNTQSLLFRRKRFCRFTVAGVEEIDYKDVD
                     TLRDFIAENGKIVPARLTGTRAIYQRQLNTAIKRARFLALLPYTDQHRV"
     gene            4039..4491
                     /gene="rplI"
                     /locus_tag="PKN51_10435"
     CDS             4039..4491
                     /gene="rplI"
                     /locus_tag="PKN51_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNT85559.1"
                     /translation="MQIILLDKVTNLGNLGEIVKVKDGYARNFLIPSGRARRATEAAK
                     AEFEARRAELEKQAAEKLAAAQAVGEKLNGQTIKLTQKAGVDGRLFGSVTNHDIADEL
                     KKQGYDVHKSQVRLPAGPIKTVGDNTITVSLHTDVSAEITVSVYGETA"
     gene            4685..6100
                     /gene="dnaB"
                     /locus_tag="PKN51_10440"
     CDS             4685..6100
                     /gene="dnaB"
                     /locus_tag="PKN51_10440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNT85560.1"
                     /translation="MSSVFPPGYADLGDDAPTDRQVAQLRVPPHSHEAESSVLGGLLL
                     DNGAWDRVGDVLVDADFYRFEHRLVYGAIGKLVAESKPADVITVHEELQRQGKGDEVG
                     GLAYLNSLAQYVPSASNIRRYAEIVRERSILRQLVSASDDIATSAFNTQGRPVAKILD
                     DAEGKIFKIGEQSSRMKQGFQAMDSLVVQLLDRVQEMADNPNDVTGVPTGFIDLDRMT
                     AGLQPGDLVVLAARPSMGKTAFAINIAEHVALHEQLPVAVFSMEMGASQLAVRIVGSI
                     GRIDQGHLRTGKLTDEEWPRLTEAIERLRNVSLHIDETPGLTPAELRANARRLARQCG
                     KLGLIVVDYLQLMSGSSSGDENRATELGEISRGLKALAKELQCPVIALSQLNRSVEQR
                     TDKRPMMSDLRESGAIEQDADIITFIYRDDYYNKDSREPGVAEIIIGKQRNGPTGTVK
                     LAFIKPLTRFESLAMGSSDDY"
     gene            complement(6194..>8970)
                     /locus_tag="PKN51_10445"
     CDS             complement(6194..>8970)
                     /locus_tag="PKN51_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85561.1"
                     /translation="LAAHAAMDDEDFERYLLTAGKGTPITPETIKSLSVGSSINSAQS
                     TYNLRPIFDLGRLKEDGRLNVDTTRSQQKTVLRVTSNKAASTSAEQLNLDVSASGSVG
                     RESFGAKASAAVHHANTRRDTKSDGTVSVQMVSANTNNLVSILSSGFSGSENFTGYLI
                     GTKLTDEQLRGYVATTESTPTGICGGKPYTTSVKVSRYKLSDTDPYANIQILTRMENV
                     FADLKAQYELCADASIQTVLVRQMTELRGKIESAIADFYAFNGDAFVSQTTSMNQAIG
                     SGTMSFTTKDGNAEEQYGASVSAQYQTLTSGVGGSVTVQYYKQHGWAEALLNVQVSAE
                     AKPAGVADTTAWVSSIQTMLKDQGSSLVPPMGNLPKDPGVKLPEPVGLRHDEEAPPDV
                     AFTSYDQWKQYQQDKKDKKGVKDEVVLEQVQQQIENEPLRINDDNPQLKATQGGDAYE
                     QLIAELDALKERATAPQAPPQGNDGNLVRFDKMFVSGFETLPYDAVIPQLRPDLDIPG
                     IDQGMGSFPNIMTAMLMSEKLGRLDSYLAFLANISVSNVTPEMSERYHQFFQKASARA
                     YDLVGLALSQGTDMTPAVLAGYKKAMLGTASAPTQSELYKSLQDIDYYNYVVDTLLDP
                     ARGKAWFVAPGGYIPMRWKNDDRGGAELVTWTGLAPARGIAPGKESVVAVDFNNPNAD
                     PLTLYRNGMKILQTPWYPVYVFNQGQAPALVFVQHFGAYQAIYGARWVTRPYDGDNPV
                     KPSLVPQWQDYQPLGTRDMRTVMTGANNSFLGQFMNWDYSVYFPNSSNDPVRLRKYNA
                     LVLHLPADYQESDGQTMYTAAHSAAVRDFSHLAYDTQSRTDATYNAFRQPTHGRKMRR
                     MSDGAVVDTTHWTLGNGALTSVMLLPLNTTTTSESLTQGLNYAPQRAPMDVVTPTSLD
                     LVNKLAVLLE"
     assembly_gap    8971..9070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9071..9273)
                     /locus_tag="PKN51_10450"
     CDS             complement(<9071..9273)
                     /locus_tag="PKN51_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85562.1"
                     /translation="MFPSRFTGGPRALPAHLVPLPCALACLLLLASCGGGSDEPEPTY
                     TSQYPTLAAHAAMDDEDFERYLLT"
     gene            complement(9312..10334)
                     /locus_tag="PKN51_10455"
     CDS             complement(9312..10334)
                     /locus_tag="PKN51_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HNT85563.1"
                     /translation="MHRTHIGLAAIAALALPLAAQAASQTFTTPGSHSWTVPPGVTHI
                     SVQALGGGGGGGDGVAIDGQSAAGGPGGLVTSGNLAVTPGQAITIVVGGGGGASGEME
                     GMGHSGGGGGASSVQVGGTWIVAGGGGGGSYYNDNSVSDTGGWGQGGHGCGGNGRPGG
                     DTGQDGGGGAGGAGSNGGAGGLAGGGGQAGSAGQAGNGGAGGAAALGADLADNGGSGA
                     GAGVGGAGGLYTPLLSGGAGGGGGWGGGGGGGSSDFTDGGGGGGGSTGGACSPGSNGG
                     RAAVRDDDDEIVDDAEDGGDGAVTITYSESVQPVPTLGQWGALALSALLGAVALRRRT
                     RITARR"
BASE COUNT         1682 a         3491 c         3596 g         1718 t
ORIGIN      
        1 cacgacagcc cctgcggcgg tcacgccgcg gtggaaaatg cgtggaactt cccacacgtc
       61 gctggcacca aatcacattc tgaaacgccg ccccggttgg gcttgttacc attgcccggc
      121 tttggcgatc tgccggcgcc gatgtgcatt gctcggatgt gatggcgctg cggcctgtgc
      181 ctatgaaatc tgctcttctc gacaagtttt cccttgcccc atggcaacgc cgcgggttcg
      241 tcacggcact ggcggcggcg ccgttctcgg cggcgctggc acagtttcgg gttgaggttt
      301 cgggtgtcgg cctgacccag ttgcccattg ccctggtccc gtttcgcggc gaagacgcgg
      361 cgccgcagaa gatcagcgcc atcgtgcgcg ccgacctgga gcgcagtggc cagttccgcg
      421 ccgtcgatgc cggcggccag tccatggacg aaaccacccg gcccgagatg tcgctgtggc
      481 gccagcgcac ggccgacgcg ctggccggtg gcagtgtgac ccgcctggcc gacggccgtt
      541 gggacgtgcg ctttcgcctg tgggacgtgg tgcgcgggca ggacctcggt ggactgggct
      601 acgtcgtgcc gcaggccgat ctgcggctgg cggcgcacaa gatcgccgac tacatctacg
      661 agaagctgac cggtgacaag ggtgtgtttt ccacccgact ggcctacgtc accaagggcg
      721 gcagccgcta cactctgtgg gtggccgact ccgacggcga aaacgcccag gccgcgctgg
      781 gcagccccga gccgatcatc tcgcccgtct ggtcgcccga cggcgggcag cttgcgtacg
      841 tgtcgttcga gtcgcgcaaa ccggtcatct acgtgcacga cgtggccagc ggccgccggc
      901 gcctgctggc caacttcaag ggctccaaca gcgcgccggc ctggtcgccc gacggccgca
      961 gtctggcggt cacgctgtcg cgcgacggtg gctcgcagct gtacctgatc agcgccagcg
     1021 gcggcgagcc ccgccgcctg acgcagtcgc aaagcatcga caccgagccc gcgttctcgc
     1081 ccgacggtgc cagcatctac ttcgtcagcg accgtggcgg cgcgccgcag atctaccgca
     1141 tgccggtggg cggcggcgcc gcgcagcgcg tcaccttcag cggcagctac aacgtctcgc
     1201 ccgccatcag tcccgacggt cgctacctgg cctatgtgtc gcgcaccaat ggctacaggc
     1261 tccacgtgat ggagttggcc agcggcacca ccaccgcggt caccgacacc gccgaggacg
     1321 agagccccag ctttgcgccc aacagtcgcc tgatcgtcta cgccacgcgc cagggcggcc
     1381 gcgaggccct catgaccacc acgctggacg gcaagatcaa ggcccgtctg gcgggccagg
     1441 gcggtgacat ccgcgaaccg gcctggggac cgtaccagcg ctgatgagcg atggccgcgt
     1501 gggtcgtgca tcgaattgtc gtgttcagtt caggagtgat ttcatgttca agcgatatct
     1561 gatggtggct gccctggcgg ccgtgctggc cggttgcggc tccaacgtca agctggatcc
     1621 gccgatcgag ggcagcgcca gcgctggcgc cagcgctgcc ggacagggcg gtgccagcgg
     1681 ccagggtgcc ggccagagca cggtgaccca ggtgcaggcg ggtgcggccg gcgctgatgc
     1741 cgcaggcccg aacggcgtgg gccgtgtgat ctacttcgat ttcgacagct ttgtcgtcaa
     1801 gtccgagtac cagtcgctgg tcgaccagca cgcgcgcttc ctgcaggcca accgtggccg
     1861 cagcgtggcg ctggaaggcc acaccgacga gcgcggcagc cgggaataca acctggcgct
     1921 gggccagaag cgtgccgagg ccgtgcgccg tgccctgacg ctcgtggggg ccagcgacgc
     1981 gcaggtggaa gcggtcagtt tcggcgagga gaagccggca gccgctggcg ccagcgaaga
     2041 tgcctatgcc aagaaccgcc gtgtcgaaat ccgctaccgc tgagcgctcg atggcgatct
     2101 tcagggtgac gagcctgcgt gccgcggtgc tggcgctggc ctgtctggcc gggcaggggg
     2161 cgcaggccca gctgttcggc gacgaccagg cgcgccaggc catcctcgat ctgcgccagc
     2221 gcttcgacca ggcggtggcg gcgcagaacc ggctggtgga cgaaaacgca cagctgcgcc
     2281 gtggcatgct ggatctgcag cagcagatcg aaaccctgcg cgcggagctg gcccgagcgc
     2341 gcggccagga cgaacagctg gcacgcgatc tggccgacct gcagaaagcg cagtcgacgc
     2401 agcagcaggg ctgggacgag cgcctgcgca agctggagca ggaggccagc gcacggatga
     2461 gcgatccgaa cgccgccgcg ggggcgcctc ccgtggccag cgacggtgcg gccgccagtg
     2521 aaaaacagga ctacgacgcc gcgctggcgg tgttccgtgc gggcgacttc aaaggcgcgc
     2581 agaccggttt tgccaatttc atcaagcgct atccgcgcag cacactggcg ccgtcggcct
     2641 tgttctggct gggcaacgcc cagtacgcca cgcgcgacta caaggaagcc atcaccaact
     2701 tccgttcgct gctcaccgcg gcgcccagcc acgcgcgggc gcccgaggcc atgctggcga
     2761 tcgccaactg ccagatcgag ctgaaggaca cacgagccgc gcgcggcacg ctggagtcgc
     2821 tgatcaagac ctacccgcag tcggaggcgg cccaggcggg caaggaccgc ctggcacgcc
     2881 tgaagtgacg ccagacggtg cccgcacgcg cctggccggt gcgcggccgg acttgcgttt
     2941 ataattattg gttgccttgc cgcaccccga tctgacgccg ggggcggttg ttgcctgagt
     3001 ccgctggcgg cctcgggccc gttcatccac aaggagcatt catgcgtcac tatgaaatca
     3061 ttttgctgat ccacccggat caaagcgagc aggttccggc catgctggag cgctacaagg
     3121 gcatgatcac cgccggcggc ggcaagatcc accgcgagga agactggggc cgtcgtcagc
     3181 tggcctacca gatcaacaag ctggccaagg cccactatct gtgcctcaac atcgaggccg
     3241 accaggccgt gctgggcgag ctggaacacg ccttccgctt caatgacgcc gtgctgcgcc
     3301 acctgacggt gcagaagaag aaggccgaga cggctccgtc ttccatgatg aagaccgtcg
     3361 agcgcgagga ggcccgcaag gcccagcagg cggaggccaa ctgacgcggc gtgcgcggtg
     3421 cctcggggca gggcggtgaa ccagttgcgc ctccaggccc gcatcgtcga aatcggtgcg
     3481 ctgcgccaca cccctgccgg cctgccggcg ctggatctcc ggctcgagca cgagtcgacc
     3541 cagcaggaag caggccagtc caggcaggtc cgggcggcgc tcagggcggt ggctctgggt
     3601 gcgatggctg aacggctggg ccggcaggtc atcggatcgc aggcctgctt cagcggtttt
     3661 ctcgccagcc cgcagcgcac caggtcggtg gtgttccaca tccaggattt tcagcaagat
     3721 cagtttcaag gagcctaaca aatggccacg ttcaagaaat tcaacaagga caagcgtccc
     3781 aagcgcaaca cccagtcgct gctgttccgt cgcaagcgct tctgccgctt cacggtggcg
     3841 ggcgtggaag agatcgacta caaggacgtc gacaccctgc gcgacttcat cgccgaaaac
     3901 ggcaagatcg tgcccgcgcg cctgaccggc acgcgcgcca tctatcagcg tcagctcaac
     3961 accgccatca agcgcgcgcg ctttctggcc ctgctgccct acaccgacca gcaccgcgtc
     4021 tgataggagg atcaaaccat gcaaatcatt cttctggaca aggtcaccaa cctgggcaac
     4081 ctgggtgaaa tcgtcaaggt caaggacggc tatgcccgca acttcctgat cccctccggg
     4141 cgtgcgcgcc gcgccaccga ggcggccaag gccgagttcg aggcgcgtcg cgccgaactc
     4201 gagaagcagg ccgccgaaaa gctggccgct gcccaggcgg tgggcgagaa gctcaacggc
     4261 cagaccatca agctgaccca gaaggccggc gtcgacggcc gtctgttcgg ctcggtcacc
     4321 aaccacgaca tcgccgacga gctgaagaaa cagggctacg acgtgcacaa atcgcaggtt
     4381 cgcctgcctg ccggcccgat caagaccgtg ggcgacaaca ccatcacggt gtcgctgcac
     4441 accgacgtga gcgccgagat cacggtctcg gtgtacggcg aaaccgcctg agcgccgccg
     4501 ctccaggccc agcccgggcc gcccgccgca gacacggcag gcggcccttg tcttttgcgg
     4561 tgccgcgatg tccacagctc tggctgcggc gttcaccggc gctgcaccgt ttgtccacag
     4621 ccttgtccag tgacagcccc ggccgcgcgc gctagcatgg gcggattgtc tgtctgcctg
     4681 ccgcatgtct tccgtatttc cccccggcta cgccgatctg ggtgacgacg cccccaccga
     4741 ccgccaggtc gcccaattgc gcgtgccgcc gcattcccat gaggccgaat ccagcgtgct
     4801 gggcggcctg ctgctggaca acggcgcctg ggatcgggtg ggcgacgtgc tggtcgacgc
     4861 cgatttctac cgtttcgagc accgtctggt gtatggcgcc atcggcaagc tggtggccga
     4921 gagcaagccg gccgacgtga tcacggtgca cgaagagctg cagcgccagg gcaagggcga
     4981 cgaggtgggt ggtctggcct acctcaactc gctggcgcag tacgtgccca gcgccagcaa
     5041 catccgccgc tacgccgaga tcgtgcgcga gcgctcgatc ctgcgccagc tggtcagtgc
     5101 cagcgacgac atcgccacca gcgccttcaa cacccagggc cggccggtgg ccaagatcct
     5161 ggacgacgcc gaaggcaaga tcttcaagat cggcgaacag agctcgcgca tgaagcaggg
     5221 ctttcaggcc atggacagcc tggtggttca gttgctcgac cgtgtgcagg agatggccga
     5281 caacccgaac gacgtcaccg gggtgccgac cggcttcatc gacctcgacc gcatgaccgc
     5341 cggcctgcag ccgggcgatc tggtggtgct ggcggcccgc ccgtcgatgg gcaagacggc
     5401 gtttgccatc aacatcgccg agcatgtggc gctgcacgag cagctgccgg tggcggtgtt
     5461 ttcgatggaa atgggcgctt cgcagctggc ggtgcgcatc gtcggttcca tcggccgcat
     5521 cgaccagggc cacctgcgca ccggcaagct gaccgacgag gaatggccgc gcctgaccga
     5581 agccatcgaa cgcctgcgca acgtcagcct gcacatcgac gaaaccccgg gcctgacgcc
     5641 ggccgagctg cgcgccaacg cgcgccggct ggcgcgccag tgcggcaagc tgggcctgat
     5701 cgtggtcgac tacctgcagc tgatgagcgg cagcagcagc ggcgacgaga accgcgccac
     5761 cgagctgggc gaaatctcgc gcggcctgaa agccctggcc aaggagctgc agtgcccggt
     5821 gatcgcgctg tcgcagctca accgcagcgt cgagcagcgc accgacaagc gccccatgat
     5881 gagcgacctg cgcgagtccg gcgccatcga gcaggacgcc gacatcatca cgttcatcta
     5941 ccgcgacgac tactacaaca aggactcgcg cgagcccggc gtggccgaga tcatcatcgg
     6001 caaacagcgc aacggcccca ccggcaccgt caagctggcc ttcatcaagc cgctcacgcg
     6061 cttcgagagc ctggccatgg gcagcagcga cgactactga ccgtttgtgg ccccagcgct
     6121 ttctggataa gcgctagcag ctatcaaaaa gtgatttttc aaacagcctg ccgttgcctg
     6181 cggcaggccc ggttcactcc agcagcacgg ccagtttgtt gacgaggtcg aggctggtgg
     6241 gggtcacaac gtccatcggc gcgcgctgag gcgcatagtt cagtccctgc gtcaggctct
     6301 cgctcgtggt ggtggtgttc agcggcagca gcatcacgga ggtgagtgcg ccgttgccca
     6361 gcgtccagtg cgtggtgtcc accaccgcgc cgtcgctcat gcgccgcatc ttgcggccat
     6421 gggtgggctg gcggaaggcg ttgtaggtgg cgtcggttct cgactgagtg tcgtaagcga
     6481 gatggctgaa atcgcgcacc gccgccgagt gcgcggcggt gtacatggtc tgcccgtcac
     6541 tctcttggta atcggcgggc agatgcagca cgagcgcgtt gtacttgcgc aagcgcacag
     6601 ggtcgttgct ggaattcggg aagtacaccg agtagtccca gttcatgaac tgccccagaa
     6661 aggaattgtt ggcgcccgtc atcaccgtgc gcatgtcccg cgtgcccagc ggctggtagt
     6721 cctgccactg gggcaccagg ctcggcttga cggggttgtc gccgtcgtag ggccgcgtca
     6781 cccaccgggc gccgtagatg gcctggtagg cgccgaagtg ctgcacgaac accagcgccg
     6841 gcgcctggcc ctggttgaag acatagaccg ggtaccaggg cgtctgcagg attttcatgc
     6901 cgttgcggta gagcgtcagc gggtcggcgt tggggttgtt gaagtccacg gccaccacgc
     6961 tttccttgcc gggggctatt ccccgtgctg gcgccagccc ggtccaggtg accagttccg
     7021 cgccgccgcg gtcgtcgttc ttccagcgca tggggatata gccgcccggc gcgacgaacc
     7081 acgccttgcc ccgggcgggg tcgagcaggg tgtccaccac gtagttgtag tagtcgatgt
     7141 cctgcaggct cttgtacagc tcgctttgcg tcggggcgga tgcggtgccg agcatggcct
     7201 tcttgtagcc ggccagcacg gcgggcgtca tgtcggtgcc ctggctcagg gccaggccca
     7261 ccaggtcgta ggcgcgtgcg gacgccttct ggaagaactg gtggtagcgc tcactcatct
     7321 cgggcgtgac gttgctgacc gagatgttgg cgagaaaggc caggtacgaa tcgagccggc
     7381 cgagcttttc gctcatcagc atggcggtca tgatgttggg gaagctcccc atgccctggt
     7441 cgataccggg tatgtccagg tcggggcgca gctgggggat gacggcgtcg tagggcagcg
     7501 tttcgaagcc gctgacgaac atcttgtcga agcgcaccag gttgccgtca ttgccctgcg
     7561 gtggggcctg cggggccgtg gcacgctcct tgagcgcgtc cagctcggcg atgagctgtt
     7621 cgtaggcgtc gccgccctgc gtggctttca gttgcggatt gtcgtcgttg atgcgcagcg
     7681 gctcgttttc gatttgctgc tgcacctgct ccagcacgac ctcgtccttg acgcccttct
     7741 tgtccttttt gtcctgctgg tactgcttcc actggtcgta ggaggtgaag gccacgtccg
     7801 gcggggcttc ctcgtcgtgg cgcaggccca ccggttcggg cagcttcacg cccggatcct
     7861 tgggcaggtt gcccatgggc ggcaccaggg agctgccctg gtccttgagc atggtctgga
     7921 tgctgctgac ccaggccgtg gtgtcggcca cgccggcggg cttggcctcg gccgagacct
     7981 gcacgttcag cagcgcctcg gcccagccgt gctgcttgta gtactgcacg gtgacactgc
     8041 cgccgacccc gctggtcagc gtctggtatt gggccgatac cgaggcgccg tactgctcct
     8101 cagcgttgcc gtccttggtc gtgaacgaca tcgtgccgct gccgatggcc tggttcatcg
     8161 aggtggtctg cgagacgaag gcgtcaccgt tgaaggcgta gaagtcggcg atggcgcttt
     8221 cgatcttgcc gcgcagctcg gtcatctggc gcaccagcac ggtctggatg gatgcatcgg
     8281 cgcacagttc gtattgcgcc ttcagatcgg cgaatacgtt ctccatgcgc gtgaggattt
     8341 ggatgttggc gtaagggtcg gtgtcggaca gcttgtagcg gctgaccttg acgctggtgg
     8401 tgtagggttt gccgccgcag atgccggtgg gcgtgctctc ggtagtggca acgtagccgc
     8461 gcaactgctc gtcggtcagc ttggtgccga tcaggtagcc ggtgaagttc tcgctgccgg
     8521 aaaagccgct gctcaggatg gagacgaggt tgttggtgtt ggccgacacc atctgcacgc
     8581 tgacagtgcc gtcggacttg gtgtcgcgcc gggtgttggc atggtgaacg gcggctgaag
     8641 ccttggcgcc gaaagattcg cggccaacgg agccagaggc cgaaacatcg aggttgagct
     8701 gttcggcgga cgtgctggcg gctttgttgc tggtgacgcg cagcacggtc ttttgctgag
     8761 aacgggtggt gtccacgttc agccggccgt cttccttgag ccgacccaga tcgaagatgg
     8821 ggcgcaggtt gtaggtggac tgggccgagt tgatgctgga gccgaccgag agcgacttga
     8881 tggtctcggg cgtgatgggg gtgcccttgc cagcggtcag caggtagcgc tcgaagtctt
     8941 cgtcgtccat ggcggcgtgc gcggccaggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn gtcagcaggt agcgctcgaa gtcttcgtcg tccatggcgg cgtgcgcggc
     9121 cagggtgggg tattgcgagg tgtaggtcgg ctccggctcg tccgagccgc cgccgcaact
     9181 ggccagcaag agcagacagg ccagggcgca aggcagcgga accagatggg cgggcaaggc
     9241 gcgcgggccg ccagtgaagc gagaggggaa catgggaatg actcctgcgg tggacatgaa
     9301 tgggtgagcg ctcaacgccg ggcggtgatc cgcgtgcgtc ggcgcagcgc aacggcgccg
     9361 agcagggcgg acagggcgag cgcgccccac tggcccagcg tgggcacggg ttgcaccgac
     9421 tcgctgtagg tgatggtgac ggcgccgtcg ccaccgtctt cggcgtcgtc gacgatctcg
     9481 tcgtcgtcgt cgcgcacggc ggcgcggccg ccgttgctgc ccgggctgca ggcgccgccg
     9541 gtgctgccgc cgcccccgcc gccgccgtcg gtgaagtcgc tcgacccgcc gccgccgccg
     9601 ccaccccagc cgccgccgcc gcccgcgccg ccgctgagca gcggcgtgta caggccaccc
     9661 gcgccaccca cgccggcgcc ggcgcccgag ccgccgttgt cggccagatc ggcgccgagt
     9721 gcggcagcgc cgcccgcacc cccgttgccg gcctggcccg ccgagccggc ctggccgccg
     9781 ccgcctgcaa ggccaccggc accgccgttg ctgccggcac cgccagcgcc gccgccaccg
     9841 tcctggccgg tgtcgccacc gggccgaccg ttgccgccgc aaccgtgacc gccctggccc
     9901 cagcccccgg tatcggagac gctgttgtcg ttgtagtagg agccgccgcc gccgccgccc
     9961 gccacgatcc aggtgccgcc cacctggacg ctgctggcgc cgccgccgcc gccggagtgg
    10021 cccatgcctt ccatctcgcc gctggcgccg ccgccgccgc ccacgacgat ggtgatggcc
    10081 tggccgggcg tgacggcgag gttgccgctg gtcaccagac cgccggggcc gcccgccgcg
    10141 gattgaccgt caattgccac gccgtcgccg ccaccgccgc caccgcccag cgcctgcacg
    10201 ctgatgtggg tgacgccggg cggcaccgtc caggagtgac tgccgggtgt ggtgaaggtc
    10261 tggctggccg cctgggcagc cagcggcagc gccagagctg cgatggcggc caggccgatg
    10321 tgtgttcgat gcatgattgt tcctgatttg atagctgtca gcgcttgtcc atcaagcgct
    10381 agagacgttt tcgataggta aaatttccat ggcgccgcgc gccacccgca tcgcacgaaa
    10441 ccggcgcggc ccaggccagg cggccgcggg gctggcgacc ccactcacac gcgccgcgtg
    10501 agagaggggg agggcgcgca tgggctaagg gcgggtcacg gcagccactt caccggcttc
    10561 gggtagctca ggtggttctt gaccacc
//
LOCUS       DAOQTZ010000090        10512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_7086_length_10512_cov_14.624080, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000090 DAOQTZ010000000
VERSION     DAOQTZ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7086_length_10512_cov_14.624080"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKN51_10460"
     CDS             complement(<1..192)
                     /locus_tag="PKN51_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNT85564.1"
                     /translation="MTATALPASTRIAFIGGGNMASAIIGGLIRQGLPAARIDVVEPF
                     EATRAALLQQHGVTALPAAG"
     gene            296..1849
                     /gene="glpK"
                     /locus_tag="PKN51_10465"
     CDS             296..1849
                     /gene="glpK"
                     /locus_tag="PKN51_10465"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNT85565.1"
                     /translation="MRYVLALDQGTTSSRAIAFDEQGRVAALAQREFAQHFPQPGWVE
                     HDADEIWVSQLAVARDCIQKLAAAQHGQAPKAIEIVAIGITNQRETTLLWERATGRPL
                     APAIVWQDRRTTARCEQLRSQGQAGPIQRQTGLVLDAYFSATKLEWLLDQVPGARARA
                     EAGELAFGTIDSWLIWQLTGGRVHATDPSNAARTMLMNIHTLDWDDQLLRLFHIPRAV
                     LPRIVPSSGVLGETAPVLFGRPIPIAGVAGDQQAATFGQACFAPGMAKNTYGTGCFML
                     MNTGHQAVPSRNRLLTTVGWQGSAAGARHRTAYCLEGSVFMAGATVQWLRDGLQMIQT
                     ASEIEALAASVPDTGDVFLVPAFAGLGAPDWDGRARGTLVGLTRGSTRAHIARAALEA
                     IALQSADIFGAMSRDSGIALTELRVDGGASRNDLLMQMQADLLGVPVVRPRVTETTAL
                     GAAYLAGLATGVWPDGAAIAAQWAVARRFEPRLPEPERHARIARWRQAVARARGWARD
                     AAGSSPSQP"
     gene            1921..3504
                     /locus_tag="PKN51_10470"
     CDS             1921..3504
                     /locus_tag="PKN51_10470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNT85566.1"
                     /translation="MDSRPQPTDRATLLARLAEPCKYDLAVVGGGATGLGVALDAAQR
                     GHRVVLVEAHDFAKGTSSRATKLVHGGVRYLAQGNVHLVREALHERVHILQSAPHLAQ
                     PLPFVMPSYRLWETPFYGVGLKMYDLLAGRAGLGATQWLGARETLRLLPGAQPQGLKG
                     GVKYWDGQFDDARLALALARTAARHGALLVNHCEAIDVLHDAGRAVGLVVRDAESGRE
                     LRLQAGCIVNATGVWVDGLRQHDARAGGQPVRPVVAPSQGAHLVVEQSFSPGDHALLV
                     PSTTDGRVLFAVPWLGKLILGTTDHPRPDVPLEPQAGADEVDFILREAGRYLRRAPTR
                     ADILSRWAGLRPLVRPADAGSDTKAISREHTILVSPSGLVSVTGGKWTTYRAMAEDVL
                     ARCTEAGVLLPRGPSRTAGLPLVGAVGAGRRTPLSAPPGLHLYGSEAETVAALPGADR
                     WLAPGLSEAMVRFAVRHEYARTVEDVLARRSRLLFLDAAEAARAAPAVARVLGEERAG
                     DPALAAFQALAGDYAGQGA"
     gene            3684..4052
                     /gene="rplN"
                     /locus_tag="PKN51_10475"
     CDS             3684..4052
                     /gene="rplN"
                     /locus_tag="PKN51_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNT85567.1"
                     /translation="MIQTESRLEVADNTGAKSVMCIKVLGGSKRRYASVGDIIKVSIK
                     EAAPRGRVKKGEIYSAVVVRTAKGIRRGDGSLVKFDGNAAVLLNNKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            4065..4382
                     /gene="rplX"
                     /locus_tag="PKN51_10480"
     CDS             4065..4382
                     /gene="rplX"
                     /locus_tag="PKN51_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNT85568.1"
                     /translation="MNKIRKGDEIIVIAGRDKGKRGTVTLRVDDERVVVDGINIVKKH
                     AKPNPMKGVTGGIIEKAMPIHQSNVAIYNKASGKADRVGIKTLQDGTRVRVFKSSGEE
                     IKA"
     gene            4391..4930
                     /gene="rplE"
                     /locus_tag="PKN51_10485"
     CDS             4391..4930
                     /gene="rplE"
                     /locus_tag="PKN51_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNT85569.1"
                     /translation="MARLQQHYREKVAPDLMKQFGYKSPMEVPRITKITLNMGVSEAV
                     ADKKVMDNAVADLTKIAGQKPVVTKARKAIAGFKIREQQAIGTMVTLRGARMYEFLDR
                     FVTVALPRVRDFRGISGRSFDGRGNYNVGVKEQIIFPEIEYDKVDALRGLNISITTTA
                     KTDAEAKALLAAFRFPFKN"
     gene            4940..5245
                     /gene="rpsN"
                     /locus_tag="PKN51_10490"
     CDS             4940..5245
                     /gene="rpsN"
                     /locus_tag="PKN51_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNT85570.1"
                     /translation="MAKVALIERELKREKLVAKYAKKHAELKAIAGDAKRSDDERAQA
                     RLALQKLPRNANPTRQRNRCEMTGRPRGTFRQFGLGRAKIRELAFEGQIPGVTKASW"
     gene            5264..5659
                     /gene="rpsH"
                     /locus_tag="PKN51_10495"
     CDS             5264..5659
                     /gene="rpsH"
                     /locus_tag="PKN51_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNT85571.1"
                     /translation="MSMSDPIADLLTRIRNAQMVAKQTVSVPSSKVKVAIAQVLKDEG
                     YIDGFEVKSEGGKSELEIALKYYAGRPVIERIERVSRPGLRVYKGRDAIPQVMNGLGV
                     AIVTTPRGVMTDRKARATGVGGEVLCYVA"
     gene            5672..6205
                     /gene="rplF"
                     /locus_tag="PKN51_10500"
     CDS             5672..6205
                     /gene="rplF"
                     /locus_tag="PKN51_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNT85572.1"
                     /translation="MSRIAKMGVTVPAGVDVQIKEDQIMVKGSGGELKLAPSALVKIA
                     MADGKLSFEPADESREANAMSGTMRQLVNNMVVGVSKGFEKKLTLVGVGFRAQAQGSK
                     LNLAIGFSHPVNFDMPAGVTVATPTPTEIVIKGANRQVVGQIAAEIRDVRPPEPYKGK
                     GIRYADERVVIKETKKK"
     gene            6217..6582
                     /gene="rplR"
                     /locus_tag="PKN51_10505"
     CDS             6217..6582
                     /gene="rplR"
                     /locus_tag="PKN51_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNT85573.1"
                     /translation="MTTKKEQRLRRARQTRIRIADKGVCRLSVNRTNLHIYATVFSAD
                     GTRVLATASTAEAEMRQALGAAGKGGNVAAAQQIGKRIAERAKAAGVDKVAFDRAGFA
                     YHGRVKALAEAAREAGLQF"
     gene            6595..7119
                     /gene="rpsE"
                     /locus_tag="PKN51_10510"
     CDS             6595..7119
                     /gene="rpsE"
                     /locus_tag="PKN51_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNT85574.1"
                     /translation="MASKFQARAQGDGPEDGLREKMIAVNRVTKVVKGGRILGFAALT
                     VVGDGDGRVGMGKGKSKEVPSAVQKAMEEARRNMLKVSLKDGTIHHNVTGHHGAATVM
                     MAPAPRGTGIIAGGPMRAVFEVLGITDIVAKSHGTSNPYNMVRATLDALRNCTTPAEV
                     AAKRGKSVEELFAA"
     gene            7134..7319
                     /gene="rpmD"
                     /locus_tag="PKN51_10515"
     CDS             7134..7319
                     /gene="rpmD"
                     /locus_tag="PKN51_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNT85575.1"
                     /translation="MTTEQKTVKVQLVRSPIGCKQSHRDTVRGLGLRKLNSVRELQDT
                     PAVRGMINKIDYLVKIV"
     gene            7331..7762
                     /gene="rplO"
                     /locus_tag="PKN51_10520"
     CDS             7331..7762
                     /gene="rplO"
                     /locus_tag="PKN51_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNT85576.1"
                     /translation="MELNSIKPADGARRARRRVGRGIGSGLGKTAGRGHKGQKSRSGG
                     YHKVGFEGGQMPLQRRLPKRGFKSASLKYNAEVTLADLERLGAAEVNLLVLKQAGLVR
                     ELAKVVKVIKTGELTRAVKLSGIGATAGAKAAIEAAGGSLA"
     gene            7801..9114
                     /gene="secY"
                     /locus_tag="PKN51_10525"
     CDS             7801..9114
                     /gene="secY"
                     /locus_tag="PKN51_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNT85577.1"
                     /translation="MATSATQIAKTGKYGDLQRRLVFLLLALVVYRIGAHIPVPGINP
                     DQLRQLFQGQQGGILNLFNMFSGGALSRFTVFALGIMPYISASIIMQLMTYVVPTFEQ
                     MKKEGEAGRRKITQYTRYGTVGLALFQSFGIAVALEASQGLVISPGWGFRLTAVVSLT
                     AGTMFLMWLGEQITERGLGNGISILIFAGIAAGLPSAIGGLLELVRTGAMSIIVALFI
                     VVLVVGVTYFVVFVERGLRKILVNYARRQVGNKVYGGQSSHLPLKLNMSGVIPPIFAS
                     SIILLPATVVGWFSAGDSMRWLKDIAATLTPGQPIYVMLYAAAIIFFCFFYTGLVFNS
                     RETADNLKKSGAFIPGIRPGEQTARYIDKILMRLTLIGAAYITAVCLLPEFLILKYNV
                     PFYFGGTSLLILVVVTMDFMAQVQNYMMSQQYESLLKKANFKTTL"
     gene            9127..9345
                     /gene="infA"
                     /locus_tag="PKN51_10530"
     CDS             9127..9345
                     /gene="infA"
                     /locus_tag="PKN51_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNT85578.1"
                     /translation="MSKDDVIQMQGEVLENLPNATFRVKLENGHVVKGHISGKMRVHY
                     IRILPGDKVTVELTPYDLSRARIVFRAK"
     gene            9395..9508
                     /gene="rpmJ"
                     /locus_tag="PKN51_10535"
     CDS             9395..9508
                     /gene="rpmJ"
                     /locus_tag="PKN51_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNT85579.1"
                     /translation="MRVSASVKKICRNCKIIRRKGVVRVICTDPRHKQRQG"
     gene            9547..9912
                     /gene="rpsM"
                     /locus_tag="PKN51_10540"
     CDS             9547..9912
                     /gene="rpsM"
                     /locus_tag="PKN51_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNT85580.1"
                     /translation="MARIAGINIPPHKHAEIGLTAIYGIGRTRARQICDACGIPYSKK
                     VKDLTDADQEKIRDQIAQFIIEGDLRRETTMNIKRLMDIGCYRGFRHRRGLPMRGQRT
                     RTNARTRKGPRKAAQALKK"
     gene            9931..10335
                     /gene="rpsK"
                     /locus_tag="PKN51_10545"
     CDS             9931..10335
                     /gene="rpsK"
                     /locus_tag="PKN51_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNT85581.1"
                     /translation="MAKSPASNAAQRVRKKVRKNIADGIAHVHASFNNTIITITDRQG
                     NALSWASSGGQGFKGSRKSTPFAAQVASESAGRAAMEQGIKNVDVEIKGPGPGRESSV
                     RALAALGIRITSISDVTPVPHNGCRPQKRRRI"
BASE COUNT         1888 a         3556 c         3506 g         1562 t
ORIGIN      
        1 gccggcggcg ggcagtgcgg tcacgccgtg ctgctgcaac agggcggcgc gcgtggcctc
       61 gaagggctcc accacgtcga tgcgcgccgc cggcagcccc tgccggatca gcccgccgat
      121 gatggcgctg gccatgttgc cgccgccgat gaaggcgatg cgggtggagg cgggcagggc
      181 ggtggcggtc atggcggtcg ggcggcgtgg cgggtgacgg agggcggtca gtgtaagcgc
      241 gccctggcgc agggcgggcg acccgggcgc agccgaatcc gctacccttg cacccatgcg
      301 ctacgttctc gcactcgatc agggcaccac cagcagccgc gccatcgcgt ttgacgagca
      361 gggccgggtg gcggcgctgg cgcagcgcga attcgcgcag cattttccgc agcccggctg
      421 ggtcgaacac gatgcggacg agatctgggt cagccagctg gcggtggccc gagattgcat
      481 acaaaaactg gctgcagcgc agcatgggca agcgccaaaa gctattgaaa ttgtagctat
      541 cggcatcacc aaccagcgcg aaaccacgct gctgtgggag cgcgccacgg gtcgtccact
      601 ggcgcccgcc atcgtctggc aggaccggcg caccacggcg cgctgcgagc agctgcgcag
      661 ccagggccag gccgggccga tccagcgtca gaccgggctg gtgctggatg cgtatttctc
      721 ggccaccaag ctcgaatggc tgctcgacca ggtgcccggc gcccgcgccc gcgccgaagc
      781 cggcgagctg gccttcggca ccatcgacag ctggctgatc tggcagctga ccggcggccg
      841 cgtgcacgcc accgacccca gcaacgccgc ccgcacgatg ctgatgaaca tccacacgct
      901 ggactgggac gaccaactgc tgcggctgtt tcacatcccg cgcgccgtgc tgccgcgcat
      961 cgtgccgtcc agcggcgtgc tgggcgagac ggcgccggtc ctgttcggcc gcccgatccc
     1021 gatcgccggc gtggccggcg accagcaggc cgccaccttc ggccaggcct gctttgcgcc
     1081 cggcatggcc aagaacacct acggcaccgg ctgcttcatg ctgatgaaca ccggccacca
     1141 ggccgtgccc agccgcaacc ggttgctcac caccgtgggc tggcagggct cggcggcggg
     1201 cgcgcgccac cgcacggcct attgcctgga aggctcggtg ttcatggccg gcgccaccgt
     1261 gcaatggctg cgcgacggcc tgcagatgat ccagaccgcc agcgagatcg aggcgctggc
     1321 ggccagcgtg cccgacaccg gcgacgtgtt cctggtgccc gccttcgccg gcctgggcgc
     1381 gcccgactgg gacggccgcg cgcgcggcac cctggtgggg ctgacgcgcg gcagcacgcg
     1441 cgcgcacatc gcccgcgccg cgctggaagc cattgccctg cagtcggccg acatcttcgg
     1501 cgccatgagc cgcgacagcg gcatcgccct gaccgagctg cgcgtggacg gcggcgccag
     1561 ccgcaacgac ctgctgatgc aaatgcaggc cgatctgctg ggcgtgccgg tggtgcgccc
     1621 gcgcgtcacc gaaaccaccg ccctgggcgc cgcctacctg gccgggctgg ccaccggcgt
     1681 ctggcccgac ggcgcagcga tcgcggcgca gtgggccgtg gcgcgacgct tcgagccgcg
     1741 gctgcccgag cccgagcggc acgcccgcat cgcccgctgg cgccaggcgg tggcgcgcgc
     1801 gcgcggctgg gcacgcgacg ccgccgggtc atcgccgtcg cagccatgag gcattgcatc
     1861 ggcattcagc gcttatccag caagcgccgc tagctatcaa aacaatagta atttcgagcc
     1921 atggacagcc gcccccagcc gaccgaccgc gccaccctgc tggcccgtct ggccgagccc
     1981 tgcaagtacg acctggccgt ggtgggcggc ggcgccaccg gcctgggggt ggcgctggac
     2041 gccgcgcagc gcggccaccg cgtggtgctg gtggaggcgc acgacttcgc caagggcacc
     2101 tcttcgcgcg ccaccaagct ggtgcatggc ggcgtgcgct acctggcgca gggcaacgtg
     2161 cacctggtgc gcgaggcgct gcacgaacgc gtgcacatcc tgcagagcgc gccgcacctg
     2221 gcgcagccgc tgcccttcgt catgccgtcg taccggctgt gggaaacgcc gttctacggc
     2281 gtcggcctga agatgtacga cctgctggcc ggccgtgccg gtctgggcgc cacccaatgg
     2341 ctgggcgcgc gcgagacgct gcgcctgctg ccgggggcgc agccgcaggg tctgaagggc
     2401 ggcgtcaaat actgggacgg ccagttcgac gacgcccgtc tggcgctggc gctggcacgc
     2461 acggcggcgc ggcacggtgc gctgctggtc aaccactgcg aggcgatcga cgtgctgcac
     2521 gacgccggcc gcgccgtcgg cctggtggtg cgtgacgccg aaagcgggcg cgaactgcgc
     2581 ctgcaggccg gctgcatcgt caacgccacc ggcgtctggg tggacgggct gcgccagcac
     2641 gacgcgcgcg ccggcggcca gccggtgcgg ccggtggtgg cgcccagcca gggcgcccac
     2701 ctggtggtgg aacagtcgtt ttcgcccggc gatcacgccc tgctggtgcc cagcaccacc
     2761 gacggccggg tgctgtttgc cgtgccctgg ctgggcaagc tgatcctggg caccaccgac
     2821 cacccgcgcc ccgacgtgcc actggagccg caagccggcg ccgacgaggt cgatttcatc
     2881 ctgcgcgaag ccggccgcta cctgcgccgc gcgcccaccc gcgccgacat cctcagccgc
     2941 tgggccggcc tgcgcccgct ggtgcggccg gccgacgccg gcagcgacac caaggccatc
     3001 agccgcgagc acaccatcct ggtgtcgccc tccggcctgg tcagcgtcac cggcggcaaa
     3061 tggaccacct accgcgccat ggccgaagac gtgctggcgc gctgcaccga agccggcgtg
     3121 ctgctgccgc gcggtccctc gcgcaccgcc gggctgcccc tggtgggcgc cgtcggcgcc
     3181 ggccggcgca cccccctgag cgcgccgccc ggcctgcacc tgtacggcag cgaggccgag
     3241 accgtggcgg ccctgcccgg cgccgatcgc tggctggcgc ccggcctgag cgaggccatg
     3301 gtccgttttg ccgtccgcca cgaatacgcc cgcaccgtcg aggacgtgct ggcgcgccgc
     3361 tcgcgcctgt tgtttctgga cgccgccgag gctgcgcgcg ccgcccccgc cgtggcgcgc
     3421 gtgctgggcg aagaacgggc gggcgacccc gcgctggcgg cattccaggc cctggccggg
     3481 gactacgccg gccagggcgc ttgacaaagc cagctcgctt tgccacaatg gcgggcttcg
     3541 cccttattgg gtgaggaatc tgccccacat cgcctggcgc atgcgcgcat gaaaagagcg
     3601 cgcggcctgg gtggcgggcc caagactgtt tcggtgtcgg cttcacgtca gaggcggccg
     3661 gatgcaagtt gggaaactta caaatgatcc agactgaatc tcgactcgag gtggccgaca
     3721 acaccggcgc caagtccgtc atgtgcatca aggtgctggg cggctccaag cgccgctatg
     3781 ccagcgtcgg cgacatcatc aaggtgagca tcaaggaagc cgcgccgcgc ggtcgcgtca
     3841 agaagggcga gatctacagc gccgtcgtgg tgcgcaccgc caagggcatc cgccgcggcg
     3901 acggttcgct cgtcaagttc gacggcaacg ccgccgtgct gctcaacaac aagctggagc
     3961 ccatcggcac ccgcatcttc ggcccggtca cgcgcgagct gcgcaccgag cgcttcatga
     4021 agatcgtgtc gctggcccct gaagtcctgt aaggaaggcg cgtcatgaac aagatccgca
     4081 agggcgacga aatcatcgtc atcgccggcc gcgacaaagg caagcgcggc acggtgacgc
     4141 tgcgcgtcga cgacgagcgc gtggtggtcg atggcatcaa catcgtcaag aagcacgcca
     4201 agcccaaccc gatgaagggc gtgaccggcg gcatcatcga gaaggccatg ccgatccacc
     4261 agtccaacgt ggccatctac aacaaggcga gcggcaaggc cgatcgcgtc ggcatcaaga
     4321 ccctgcagga cggcacgcgc gtgcgtgtct tcaagtccag cggcgaagaa atcaaggcct
     4381 gaggaccatc atggcacgac tgcaacaaca ctaccgcgag aaagtggcgc ccgatctgat
     4441 gaagcagttc ggctacaagt cgcccatgga ggtgccgcgc atcaccaaga tcacgctcaa
     4501 catgggcgtg agcgaggccg tggccgataa gaaggtgatg gacaacgccg tcgccgacct
     4561 gaccaagatc gccggccaga aaccggtggt caccaaggcc aggaaggcca tcgccggctt
     4621 caagatccgc gagcagcagg ccatcggcac catggtcacc ctgcgcggcg cccgcatgta
     4681 cgaattcctc gaccgcttcg tcaccgtggc gctgccgcgc gtgcgtgact tccgcggtat
     4741 ttccggtcgt tccttcgatg gtcgcggtaa ctacaacgtc ggcgtcaaag agcagatcat
     4801 cttcccggaa atcgagtacg acaaggtcga cgccctgcgc ggcctgaaca tcagcatcac
     4861 gaccacggcc aagaccgacg cagaagccaa ggcgctgctg gcggctttcc gtttcccgtt
     4921 caagaactga ggtaaggcca tggcaaaagt agctttgatc gagcgcgaac tcaagcgcga
     4981 gaaactggtc gccaagtacg ccaagaagca cgccgagctg aaggccatcg ccggcgacgc
     5041 caagcgcagc gacgacgagc gcgcgcaggc tcgtctggcg ctgcagaagc tcccgcgcaa
     5101 cgccaacccc acgcgccagc gcaaccgctg cgaaatgacc ggccgcccgc gcggcacctt
     5161 ccgtcagttc ggtctcggcc gcgccaagat ccgcgaactg gccttcgaag gccagattcc
     5221 cggcgtcacc aaggccagct ggtaatcggc aggagtaagc aacatgagca tgagtgatcc
     5281 catcgccgat ctgctgaccc gcatccgcaa tgcgcagatg gtggccaagc agaccgtgtc
     5341 ggtgccgtcg tccaaggtca aggtggccat cgcccaggtc ctgaaggacg agggctacat
     5401 cgacggcttc gaagtcaaga gcgaaggcgg caagtccgaa ctggaaatcg ccctcaaata
     5461 ctacgccggc cgcccggtga tcgagcgcat cgagcgcgtc agccgtcccg gcctgcgcgt
     5521 ctacaagggc cgtgacgcca ttccccaggt catgaatggc ctgggcgtgg ccatcgtcac
     5581 caccccgcgc ggcgtgatga ccgatcgcaa ggcgcgcgcc accggtgtcg gtggcgaagt
     5641 gctgtgctac gtggcgtaag gagaaatcga aatgtcgcgt attgcaaaaa tgggcgtcac
     5701 cgtccccgcg ggcgtggatg ttcagatcaa ggaagaccag atcatggtca agggcagcgg
     5761 cggcgagctc aagctggccc ccagcgccct ggtgaagatc gccatggccg acggcaagct
     5821 gtcgttcgag ccggccgacg agtcgcgcga ggccaacgcc atgagcggca ccatgcgcca
     5881 gctggtcaac aacatggtgg tcggcgtcag caagggtttc gagaagaagc tcaccctggt
     5941 cggcgtgggc tttcgcgccc aggcccaggg cagcaagctg aacctggcga tcggcttctc
     6001 gcatccggtc aacttcgaca tgccggccgg cgtgacggtg gccacgccga cgccgacgga
     6061 aatcgtgatc aagggcgcca accgccaggt ggtgggccag atcgcggccg agattcgtga
     6121 cgtgcgtccg cccgagccct acaagggcaa aggcatccgc tacgcggacg agcgcgtcgt
     6181 catcaaggaa accaagaaga agtaaggacc tgcatcatga cgaccaagaa agagcagcgc
     6241 cttcgccgtg cccgccagac gcgcatccgc atcgccgaca agggtgtgtg ccgcctgagc
     6301 gtgaaccgca ccaacctgca tatctacgcc accgtgttct cggccgatgg cacgcgcgtg
     6361 ctggccacgg cctcgaccgc cgaagccgag atgcgtcagg cgctgggcgc cgccggcaag
     6421 ggtggcaacg tggccgccgc gcagcagatc ggcaagcgca tcgccgagcg cgccaaggcc
     6481 gccggtgtcg acaaggtagc gttcgaccgc gccggtttcg cctaccacgg ccgtgtcaag
     6541 gcgctggctg aagccgcgcg cgaagcgggc ctgcagttct aaaggaccaa cgagatggca
     6601 agcaagtttc aagcacgcgc gcagggcgat ggtcccgagg acggcctgcg cgagaagatg
     6661 atcgcggtca accgcgtcac caaggtggtc aagggtggcc gcatcctggg ttttgccgca
     6721 ctgaccgtgg tgggcgacgg cgatggccgc gtcggcatgg gcaagggcaa atccaaggaa
     6781 gtgccgtcgg ccgtccagaa ggccatggaa gaggcgcgtc gcaacatgct gaaggtgtcg
     6841 ctcaaggacg gcaccattca ccacaacgtg accggccacc acggcgccgc caccgtcatg
     6901 atggcgccgg cgccgcgcgg taccggcatc atcgccggcg gcccgatgcg cgccgtcttc
     6961 gaggtgctgg gcatcaccga catcgtggcc aagagccacg gcacctccaa cccgtacaac
     7021 atggtgcgcg ccacgctgga tgcgctgcgc aactgcacca cgcccgccga agtggcggcc
     7081 aagcgcggca agtcggtcga agaactgttt gccgcctgat ggggagtcaa gacatgacca
     7141 ctgaacagaa aaccgtcaag gtgcaactgg tgcgcagccc gatcggctgc aagcagtcgc
     7201 accgcgacac cgtgcgcggc ctgggcctgc gcaagctgaa cagcgtgcgc gagctgcagg
     7261 atacgcctgc ggtgcgcggc atgatcaaca agatcgacta cctggtcaag atcgtctgag
     7321 gaatcggatc atggaactca acagcatcaa gcccgccgac ggcgcgcgtc gtgcccgtcg
     7381 ccgcgtcggt cgcggcatcg gctccggcct gggcaagacc gccggccgtg gccacaaggg
     7441 tcagaagtcg cgcagcggcg gctaccacaa agtcggcttc gaaggcggcc agatgcccct
     7501 gcagcgccgt ctgcccaagc gcggcttcaa gtcggcctcg ctgaagtaca acgccgaggt
     7561 cacgctggcc gatctggaac gcctgggtgc cgccgaagtc aacctgctgg tgctcaagca
     7621 ggccggtctg gtgcgcgagc tcgccaaggt ggtcaaggtc atcaagaccg gtgaactgac
     7681 ccgtgccgtc aagctgtcgg gcattggcgc caccgctggc gccaaggcgg cgatcgaggc
     7741 tgccggtggt tcgctggcct gagccggctc ccgcttgcat ttcttgaaag tgactgagta
     7801 gtggccacca gcgcaacgca aattgccaag accggcaagt acggcgacct gcagcgtcgg
     7861 ctggtgtttc tgctgctggc gctggtcgtg taccggatcg gtgcgcacat tcccgtgccc
     7921 ggcatcaacc cggatcagct gcgtcagctg ttccagggcc agcagggcgg catcctgaac
     7981 ctgttcaaca tgttctcggg tggcgcgctg tcgcgcttca cggtgttcgc gctgggcatc
     8041 atgccctaca tctccgcgtc gatcatcatg cagttgatga cctacgtggt gccgaccttc
     8101 gagcaaatga agaaggaagg cgaggcgggc cgtcgcaaga tcacccagta cacgcgctat
     8161 ggcacggtgg gcctggcgct gttccagtcg ttcggcatcg ccgtcgcgct ggaggcgtcg
     8221 cagggcctgg tgatcagccc gggctggggc tttcgcctga cggcggtggt cagcctgacg
     8281 gccggcacca tgttcctgat gtggctgggc gagcagatca ccgagcgcgg cctgggcaac
     8341 ggcatctcga tcctgatctt cgccggcatc gccgctggtc tgccgagcgc catcggcggc
     8401 ctgctggagc tggtgcgcac gggggccatg agcatcatcg tggcgctgtt catcgtcgtg
     8461 ctggtggtgg gcgtgaccta cttcgtggtg ttcgtcgagc gcggactgcg caagattctg
     8521 gtcaactacg cgcgccgcca ggttggcaac aaggtgtatg ggggtcagtc ctcgcacctg
     8581 ccgctcaagc tcaacatgtc gggcgtgatc ccgccgatct tcgcctcgtc gatcatcctg
     8641 ctgcccgcca ccgtggtcgg ctggttcagt gccggcgact cgatgcgctg gctcaaggac
     8701 atcgccgcca cgctgacgcc ggggcagccg atctacgtca tgctgtatgc ggcggccatc
     8761 atcttcttct gtttcttcta caccggcctg gtgttcaaca gccgcgagac ggccgacaac
     8821 ctgaagaaaa gcggtgcctt cattccgggc atccgtccgg gtgagcagac cgcgcgctac
     8881 atcgacaaga tcctgatgcg tctgaccctg atcggggcgg cctacatcac ggcggtgtgt
     8941 ctgctgccgg agttcctgat cctgaagtac aacgtgccgt tctacttcgg tggtacctcg
     9001 ctgctgatcc tggtcgtcgt gaccatggat ttcatggcgc aggtgcagaa ttacatgatg
     9061 agtcaacagt acgaatcgct gctcaagaag gccaacttca agaccacgct ttgaccgctg
     9121 accgccatgt cgaaggatga tgtcatccag atgcagggcg aggttctgga gaacttgccc
     9181 aacgccacgt tccgggtaaa actggaaaac gggcacgtgg tcaagggcca catttcggga
     9241 aagatgcggg tgcattacat ccgcatcctc cccggtgaca aggtgacggt ggaactgacg
     9301 ccgtacgacc tgtcgagggc acgcattgtg ttccgtgcga agtgaagcag gtgcttccgg
     9361 ttcggatgga atgaaagagt tttaggagag tgaaatgaga gtttcagctt cggtcaagaa
     9421 gatctgccgc aattgcaaga tcatccgccg caagggtgtg gtgcgcgtga tctgcaccga
     9481 cccgcgtcac aagcagcgcc agggttgagc gccgcctgca cgagcattct gaaggattag
     9541 aagaccatgg cacgtattgc tggtatcaac attccgccgc acaaacatgc ggaaatcggc
     9601 ctgacggcga tctacggcat cggccgcacg cgcgcacgcc agatctgcga cgcctgcggc
     9661 atcccctact ccaagaaggt caaggacctg accgacgccg accaggagaa gatccgcgac
     9721 cagatcgccc agttcatcat cgagggtgac ctgcgccgcg agaccacgat gaacatcaag
     9781 cgcctgatgg acatcggctg ctaccggggc ttccgccatc gccgcggtct gcccatgcgt
     9841 ggccagcgca cgcgcaccaa cgcccgcacc cgcaagggcc cgcgcaaggc tgcccaggcg
     9901 ctgaagaaat aaaccaagga acgctaagac atggccaaat cacccgccag caacgccgcc
     9961 cagcgcgtgc gcaagaaggt ccgcaaaaac atcgcggatg gcatcgcaca cgtgcacgcg
    10021 tcgttcaaca acacgatcat caccatcacc gaccgccagg gcaacgcact gtcctgggct
    10081 tcgtcgggtg gccagggctt caagggttcg cgcaagtcca cgccgttcgc ggcccaggtg
    10141 gcttccgaat cggccggtcg cgcggccatg gagcagggca tcaagaacgt ggacgtggag
    10201 atcaagggtc cgggcccggg ccgcgagtct tccgtgcgcg cgctggcggc cctgggcatc
    10261 cgcatcacct cgatttccga cgtgacgccg gtgccgcaca acggctgccg cccgcagaag
    10321 cgccgccgca tctgatggcg ctcggcgccg gaatgcccgg gcgcctcatg tttttctagc
    10381 aagaccaccg ccaccagtgc ttgcaccggt ggctcctccg gcacagggcc ggagcagacc
    10441 aacccaagga aattgacgtg gcacgttacc tcggccccaa ggccaaactc tcccgccgtg
    10501 aaggcaccga cc
//
LOCUS       DAOQTZ010000091        10476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_7122_length_10476_cov_14.370214, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000091 DAOQTZ010000000
VERSION     DAOQTZ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10476
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7122_length_10476_cov_14.370214"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKN51_10550"
     CDS             complement(<1..520)
                     /locus_tag="PKN51_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT85582.1"
                     /translation="MSNLPGLNFQLGDDIDALRDAVRDFAQAEIAPRAAQIDRDNQFP
                     PDLWPKLGALGLHGMTVKEAFGGTEMGYLAHIVAMEEVSRASASVGLSYGAHSNLCVN
                     QIHRNGSEAQKKKYLPRLVSGEHVGALAMSEPNAGSDVVSMKLRAEKKNGYYVLNGSK
                     MWITNGGDADTLV"
     gene            complement(548..991)
                     /locus_tag="PKN51_10555"
     CDS             complement(548..991)
                     /locus_tag="PKN51_10555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNT85583.1"
                     /translation="MTDAADPSAIFQRVAESFARQGMMRHLGAELRRVEAGRVEIWLP
                     YSDRVTQQQDGFHGGAMGAVADIAGGYAGLTLAPEGMEVVTAEYKINFLGSYRGGALR
                     AIGRVVKPGRRLIVTSAEVTHIDDAGQEGPCALMQQTLVPVPKQY"
     gene            complement(1028..1453)
                     /locus_tag="PKN51_10560"
     CDS             complement(1028..1453)
                     /locus_tag="PKN51_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT85584.1"
                     /translation="MATTYRISDLAREFDLTPRAMRFYEDLGLLRPERTGPGGRQRVY
                     SSRDRTRLKLTLRAKRLGLSLTEARELIDMYDSPRDTGPQLSRYLQVLGQHRAQLEAR
                     LAELQATLAEVAEQEQEARALLARLPGQGNRRPRRDAAD"
     gene            1577..2656
                     /locus_tag="PKN51_10565"
     CDS             1577..2656
                     /locus_tag="PKN51_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT85585.1"
                     /translation="MSLAAELERALHYPLGDQLPAPGCAVELRPGVKWLRMGLPFALD
                     HINLWLLRDRFQGREGWTVVDCCIDRPESRALWQQVFEQELEGLPVLRVLVTHMHPDH
                     IGLAHWLCERWSTPEHDCRLWISGADYQAARYGCEVVNSFGGERLADYFRSHGMTDPG
                     DLEQVRHRKSYYRGLVPAVPPQYARLRDGQPLVIGGHAWRCIAGWGHAPEHMALHCEA
                     LGILISGDMVLPRISTNVSVYEMEPEADALTLFLDSIDRFMPLPEDTLVLPSHGKPFQ
                     GLHTRIQQLHDHHRERLVEVMQAARERPVSAYDVLPVLFRRPLDLHQTTFALGESVAH
                     LHRLWHGGQLARTRDAEGIWRFAPV"
     gene            2757..3527
                     /locus_tag="PKN51_10570"
     CDS             2757..3527
                     /locus_tag="PKN51_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT85586.1"
                     /translation="MNTQQLFSLEGRSALVTGGSRGIGRMIAEGFLRAGARVYISARK
                     AEACDATARELSAIGPCVSLPADVSTLAGIQQLVAAYQQHEPSLDILVNNAGAAWGAP
                     YAEFPESGWDKVADLNMKTPFFLTQALTPMLKAAATDHLAKVIHIASIDGISVNPWET
                     YSYAASKAGLIHLTKRMALKLAPERIIVSAIAPGPFASEMNQSARDHADEVAARTPAG
                     RIGTPEDMAGAAIYLASRAGDYVVGSTLVVDGGVAWAR"
     gene            complement(3616..4533)
                     /locus_tag="PKN51_10575"
     CDS             complement(3616..4533)
                     /locus_tag="PKN51_10575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNT85587.1"
                     /translation="MRQLLLDLFGQGAEPSPQPPHSTSNQAPALDGQAQTAINNIVHG
                     GDATALARPTVTGFQHPQANRRIVLGGTPVAYLFRRGKRRTIGLSIGAEGLQVSAPRW
                     TPLTEIEAALHDKARWVLAKLAEARQRQAAQQGARIDWRDGAALPYLGAPLTLRLDPR
                     QQHGRAGAVLQSALPEGPILWLGLPASASAQQVQDVAQAWLLGQARRLFAARLDHFAP
                     LMGVRWQRLTLSSAGTRWGSASADGSIRLNWRLIHLDAALIDYVVVHELAHLHEMNHS
                     PRFWQHVGRVLPDCHERRVALKRSVVPTA"
     gene            complement(4545..5318)
                     /locus_tag="PKN51_10580"
     CDS             complement(4545..5318)
                     /locus_tag="PKN51_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT85588.1"
                     /translation="MNLIRSVLHLLWMVVTVIPWATLVVLLAPFVGKPRLYRICRAWL
                     DLTVRAGRWILGIENRVSGLQHLPPDPAAGAVLLVKHQSAWETFCMPPLMPHPLAYVF
                     KRELLSIPFFGWAIGRLDMIHIDRGQRIEAFHKVVEQGRRLLGQGVWVIMFPEGTRIP
                     RGEKGSYQSSGARLAVECGVPIIPVAVTSARCWPRKAFVKRPGVVDISIGPPIDTRGR
                     KPHELMREVESWIEAEMLRLDPEAYPGGAAVRSSVRPSA"
     gene            complement(5386..5955)
                     /gene="gmhB"
                     /locus_tag="PKN51_10585"
     CDS             complement(5386..5955)
                     /gene="gmhB"
                     /locus_tag="PKN51_10585"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNT85589.1"
                     /translation="MKLVILDRDGTICVEREGYLQSPADWEPLPGALEAIAQLNQAGW
                     QVVIAANMPGLGRGLFDMAALNAIQARMHKQLAAVGGKVDAMFFCPHTADEACSCRKP
                     LPGLFQQIGERYRVDLRQVHAVGDSERDMQAAHAAGCVPHLVLTGLHAPSHTDPLPPA
                     YPPGTRVHADLAAFASALVGAPDAAPGPA"
     gene            complement(5964..6587)
                     /locus_tag="PKN51_10590"
     CDS             complement(5964..6587)
                     /locus_tag="PKN51_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85590.1"
                     /translation="MSPGPAAAATTGLGWRGLTWLLAPPLAVLLLRALLQALAEADAD
                     GLQALHPATLVSDPAQALWAAARPAVFTGLGLLGAVLGLRLAGRAALRRHGWARLRPW
                     ALALWLLLWLGVGAGLVASQLNRAGRRPLPAQEVRVLLLRDVPASARGPGGTEVYFEL
                     APAGAPQRLLAEGQPPTAFAPGRPARLDWQAGRWWGRWGRLTPGGGG"
     gene            complement(6584..8728)
                     /gene="glyS"
                     /locus_tag="PKN51_10595"
     CDS             complement(6584..8728)
                     /gene="glyS"
                     /locus_tag="PKN51_10595"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNT85591.1"
                     /translation="MTTQNLLVELFVEELPPKALRQLGEAFATTLAAQLQAQGLTAAD
                     SRVTPYASPRRLAAHITHVAAKAADRAVQLKLMPVSVGLDAQGQATPALLKKLQALGA
                     EPTVVPQLKRQPDGKAEALFLDSVVTGATLDSGLQKALDEAIARLPIPKVMTYQLQAG
                     CARPGWDSVQFVRPAHGLVALHGETVVPVTALGLRAGRATHGHRFEAASDPVELASAD
                     DYALDLRQQGAVIASFEERRATLEAQIKAAATQAGTALTAIKDEALLDEVTALVERPN
                     VLVCAFEPEFLQVPQECLILTMKANQKYFPLLDAQGRLTHRFLVVSNITPDDPSAVIA
                     GNERVVRPRLADAKFFFDQDRKKSLDSRVPQLAQVVYHNQLGTQGERVERVRAIARQI
                     GQQLGGAALAQAADTAARLAKADLLTDMVGEFPELQGVMGGYYARHDGLSEDIALAIE
                     DHYKPRFAGDELPRGQVGTVVALADKLETLVGMFGIGNLPTGDRDPFALRRHALGVIR
                     MLIEQALPLALPELIELAAQTFAGKADFNPSRVKDELAGKFIYDRLAGSLREQGFSAQ
                     EVDAVLALRPPRLADVHPHLQAVRAFAALPEAPALAAANKRIGNILKKAEAESPVHPH
                     VSELLLQEPAEQALYAAMQQVLPQADAQLARGDYTASLQSLAALRAPVDAFFDHVMVN
                     AEQADLRLNRQGLLKTLHQAMNRVAELARLAA"
     gene            complement(8740..9660)
                     /gene="glyQ"
                     /locus_tag="PKN51_10600"
     CDS             complement(8740..9660)
                     /gene="glyQ"
                     /locus_tag="PKN51_10600"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNT85592.1"
                     /translation="MLTFQQIILSLQQYWADQGCALLQPYDMEVGAGTSHTATFLRAI
                     GPEPWRAAYVQPSRRPKDGRYGDNPNRLQHYYQYQVVLKPAPANILELYLGSLQALGF
                     DLKKNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCRPATGEITY
                     GLERLAMYLQGVDNVYNLVWTEGPDGSKLTYGDVYHQNEVEQSTYNFEHSDADFLFTA
                     FAAHERQALHLIGQQLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRTLARA
                     VAQSYYDSRQRLGFPLAPRAWVEQIEKPAA"
     gene            complement(9767..>10476)
                     /locus_tag="PKN51_10605"
     CDS             complement(9767..>10476)
                     /locus_tag="PKN51_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNT85593.1"
                     /translation="GAAAQQIAWGMQDVMFAGGGEELSWGMAMLFDGMGAMSSKYNAT
                     PDKASRAYDAHRDGFVIAGGGGAVVLESLEHAQARGATILAEVVGFGATSDGEDMVAP
                     SGDGAIACMRQAIDGLDAPIDYINTHGTSTPVGDMQEVRAMRAVFGDAVPPFSSTKSL
                     TGHSLGATGVQEAIYCLIMLQKGFIAGSVNVETPDPELGAMPLVTATRDARLSTVLSN
                     SFGFGGTNASLVLRRWP"
BASE COUNT         1507 a         3646 c         3728 g         1595 t
ORIGIN      
        1 ccaccagggt gtcggcgtcg ccgccgttgg tgatccacat cttgctgccg ttgagcacgt
       61 agtagccgtt cttcttctcg gcgcgcagct tcatgctgac cacgtcgctg ccggcgttcg
      121 gctcgctcat ggccagcgcc ccgacatgct cgccgctcac cagcctgggc aggtatttct
      181 tcttctgcgc ttcgctgccg ttgcggtgga tctggttgac gcacaggttg gagtgcgcac
      241 cgtaggacag accgaccgag gccgaggcgc gcgagacctc ttccatggcc acgatgtggg
      301 ccagatagcc catctcggtg ccgccgaacg cttccttcac ggtcatgccg tgcaggccca
      361 gcgcacccag cttgggccac aggtcgggcg gaaactggtt gtcgcggtcg atctgggcgg
      421 cgcgcggggc gatttcggcc tgcgcgaagt cgcgcacggc gtcgcgcagc gcgtcgatgt
      481 cgtcaccaag ctgaaagttc aggccgggca ggttgctcat ggggtctcct tgacaagatg
      541 gatgggttca gtactgcttg ggcacgggca ccagggtctg ctgcatcagg gcgcaggggc
      601 cttcctggcc ggcgtcgtcg atgtgtgtga cttcggcgct ggtgacgatc aggcgccggc
      661 cgggtttgac cacgcgcccg atggcgcgca acgcgccgcc ccggtagctg ccgagaaagt
      721 tgatcttgta ttcggccgtg accacttcca tgccctccgg cgccagcgtc aggccggcgt
      781 agccgccggc gatgtcggcc accgcgccca tggcgccgcc gtgaaagccg tcctgctgct
      841 gcgtcacgcg gtcggaatac ggcagccaga tctcgacccg cccggcctcg acccggcgca
      901 gctcggcacc caggtggcgc atcatgccct gacgggcaaa gctctcggcc acgcgctgga
      961 agatggcgga agggtcggcg gcgtcggtca tggcaaagct cgattgtgat tgacgtttac
     1021 gtaaacgtca atcagcggcg tctcggcgcg gcctgcggtt gccctgcccc ggcaggcgcg
     1081 ccagcagcgc gcgcgcctcc tgctcctgct cggccacctc ggccagcgtg gcctgcaact
     1141 cggccagccg tgcctccagc tgggcgcggt gctggcccag cacctgcagg taccggctca
     1201 gctgcgggcc ggtgtcgcgc gggctgtcgt acatgtcgat cagctcacgc gcctcggtca
     1261 gcgacagccc caggcgcttg gcgcgcagcg tcagcttcag gcgtgtgcgg tcgcggctgg
     1321 aatagacgcg ctgacggccg ccggggccgg tgcgctcggg ccgcagcaga cccaggtcct
     1381 cgtagaagcg catcgcgcgc ggcgtcagat cgaactcgcg tgccagatcg ctgatgcggt
     1441 aggtggtggc catgggtgtc gggggctgtc gcgcaggcgc cgcgtgcggc gcgactcctt
     1501 catagaatgg tccggccggt tgacgtgcgc gtcggtcgtg cccgtcatcc taacgcagcc
     1561 gcctgctccc cagcccatgt cacttgccgc cgaactcgaa cgtgccctgc actacccgct
     1621 gggtgaccag ctgcccgcgc ctgggtgcgc cgtcgaactg aggccgggcg tcaaatggct
     1681 gcgcatgggc ctgccgttcg cgctcgacca catcaacctt tggctgctgc gcgaccgctt
     1741 tcagggccgc gaaggctgga ccgtggtgga ctgctgcatc gaccggcccg aatcgcgcgc
     1801 gctgtggcag caggtgttcg agcaggagct ggagggcctg ccggtgctgc gcgtgttggt
     1861 cacccacatg catcccgacc acatcgggct ggcgcactgg ttgtgcgagc gctggagcac
     1921 gcccgaacac gactgccgcc tgtggatcag cggcgccgac taccaggccg cgcgctacgg
     1981 ctgcgaggtg gtcaacagct ttggcggcga gcgtctggcc gactacttcc gcagccacgg
     2041 catgaccgac ccgggcgacc tggagcaggt gcgccaccgc aagagctact accgcggcct
     2101 ggtgccggcg gtgccgccgc agtacgcgcg cctgcgcgac ggccagccgc tggtcatcgg
     2161 cggccacgcc tggcgctgca tcgccggctg gggccacgcg cccgaacaca tggcgctgca
     2221 ctgcgaggcg ctgggcatcc tgatcagcgg cgacatggtg ctgccacgca tctcgaccaa
     2281 cgtcagcgtc tatgaaatgg agcccgaggc cgatgcgctg acgctgtttc tggactccat
     2341 cgaccgtttc atgccgctgc ccgaggacac gctggtgctg ccctcgcacg gcaagccctt
     2401 ccagggcttg cacacgcgca tccagcagct gcacgaccac caccgcgagc gcctggtcga
     2461 ggtgatgcag gcggcgcgcg agcggcccgt gagcgcctac gacgtgctgc cggtgctgtt
     2521 caggcgcccg ctggacctgc accagaccac ctttgccctg ggcgagtcgg tggcgcacct
     2581 gcaccgtctg tggcacggtg gccagctggc gcgcacacgc gacgccgagg gcatctggcg
     2641 ctttgcgccg gtgtgagact cggtacgccg aaccaccacc accggaggcg aaggactcga
     2701 actcaaagtc cgacagcctc ccagacgcag ccctaccgta tggagaaccc ggcatgatga
     2761 acacacagca gctcttttcc ctggagggcc gcagcgccct ggtcaccggc ggctcgcgcg
     2821 gcattgggcg catgatcgcc gagggtttct tgcgcgcggg cgcacgggtc tacatttcgg
     2881 cgcgcaaggc cgaggcgtgc gatgccacgg cgcgcgagct atcggccatc gggccctgcg
     2941 tgtcgctgcc ggccgacgtg tccacgctgg cgggcatcca gcaattggtc gcggcgtacc
     3001 aacagcacga gcccagcctg gacatcctgg tcaacaacgc tggtgcggcc tggggcgcgc
     3061 cgtatgccga gttcccggaa agcggctggg acaaggtggc cgacctgaac atgaagacgc
     3121 cgttctttct cacgcaagcg ctcaccccca tgctcaaggc cgccgccacc gaccacctgg
     3181 ccaaggtgat ccacatcgcc tcgatcgacg gcatcagcgt caacccctgg gagacgtatt
     3241 cctatgccgc cagcaaggcc ggcctgatcc acctaaccaa gcgcatggcg ctcaagctgg
     3301 cgcccgagcg catcatcgtc agcgccatcg cgcccggccc gttcgcgtcg gagatgaacc
     3361 agagcgcgcg cgaccatgcc gacgaggtgg cggcacgcac cccggccggg cgcataggca
     3421 cgccggaaga catggccggc gccgccatct atctcgcctc gcgcgccggc gactacgtgg
     3481 tcggctcgac cctggtggtc gatggcgggg tggcctgggc gaggtgatga ggcggggtcg
     3541 cgctcggcac gatcaccgat gacaaatgcc ttcgccgcgc ttcgatgacc caggcgggga
     3601 gcgcccgcgg acccgtcagg cggtgggcac cacgctgcgc ttgagcgcca cgcgccgctc
     3661 gtggcagtcg ggcaacacgc gccccacgtg ctgccagaag cgcgggctgt ggttcatctc
     3721 gtgcaggtgc gccagctcgt gcaccaccac gtagtcgatc agcgccgcgt ccaggtggat
     3781 caggcgccag ttcaggcgga tcgagccgtc ggcgctggcc gagccccagc gcgtgccggc
     3841 cgatgacagc gtcagccgct gccagcgcac gcccatcagc ggcgcaaagt gatcgagccg
     3901 cgccgcaaac agccgccgcg cctgccccag cagccaggcc tgggcgacgt cctgcacctg
     3961 ctgcgcgctg gcgctggcgg gcaggcccag ccacaggatg gggccttcgg gcagtgcgct
     4021 ctgcagcacg gcgccggcgc ggccatgctg ctgacgcggg tccagccgca gcgtgagcgg
     4081 cgcgcccagg tacggcagcg ccgcgccatc gcgccagtcg atgcgcgcgc cctgctgcgc
     4141 ggcctggcgc tggcgggcct cggccagctt ggccagcacc cagcgcgcct tgtcgtgcag
     4201 ggcggcttcg atctcggtca gcggcgtcca gcgcggggcg ctgacctgca ggccctcggc
     4261 gccgatggac aaaccgatgg tgcgccgctt gccgcgccga aacagatagg ccaccggcgt
     4321 gccacccagc acgatgcggc gattggcctg ggggtgctga aagccggtga cggtgggcct
     4381 cgcgagcgcc gttgcgtcgc cgccatgaac tatattattg atagctgtct gcgcttgccc
     4441 atcaagcgct ggagcctgat ttgacgtgga atgtggcggc tgcggcgacg gctcggcgcc
     4501 ctggccgaac agatcgagca gcaactggcg catgggctgt cgcgctaggc gctggggcgc
     4561 acgctggagc gcacggcggc gccgccggga taggcctcgg ggtccaggcg cagcatttcg
     4621 gcctcgatcc aggactcgac ctcgcgcatc agctcgtgcg gcttgcggcc gcgggtgtcg
     4681 atgggcgggc cgatggagat gtcgacgacg cccggtcgct tgacgaaggc cttgcgcggc
     4741 cagcagcgcg ccgaagtgac ggcgaccggg atgatgggca cgccgcattc gaccgccagg
     4801 cgcgcgccgc tgctctggta gctgcccttt tcgccgcgcg ggatgcgcgt gccctcgggg
     4861 aacatgatca cccacacgcc ctgacccagc aggcgccggc cctgctcgac caccttgtga
     4921 aaggcttcga tgcgctggcc gcggtcgatg tggatcatgt ccagccgacc gatggcccag
     4981 ccgaagaacg ggatcgacag cagttcgcgc ttgaacacat aggccagcgg atgcggcatc
     5041 agcggcggca tgcagaacgt ttcccaggcc gactggtgtt tgaccagcag cacggcgccg
     5101 gcggccgggt ccggcggcag gtgctgcagc ccgctgacgc ggttctcgat gcccaggatc
     5161 cagcggccgg cgcgcaccgt cagatcgagc caggcgcggc agatgcggta caggcgcggc
     5221 ttgccgacga agggcgccag cagcaccacc agcgtggccc aggggatgac ggtgacgacc
     5281 atccacagca ggtgcagcac ggagcggatc aggttcatga acgatcggag gttggcgccc
     5341 gtcgggtatg cgcgagcagc tatgaataac ggagcaaacg aggcctcagg cggggcctgg
     5401 cgcggcatcg ggcgcgccga ccagggcgct ggcgaaggcc gccaggtcgg cgtgcacgcg
     5461 cgtgccgggc ggataggccg gcggcagcgg gtcggtgtgg ctgggggcgt gcaggcccgt
     5521 cagcaccagg tgcggcacgc agccggccgc gtgggcggcc tgcatgtcgc gttcgctgtc
     5581 gcccaccgca tgcacctggc gcagatcgac ccggtagcgc tcgccgatct gctggaacag
     5641 cccgggcagc ggcttgcggc agctgcacgc ctcgtcggcc gtgtgcgggc agaagaacat
     5701 ggcgtccacc ttgccgccca cggccgccag ctgcttgtgc atgcgcgcct ggatggcgtt
     5761 gagcgctgcc atgtcgaaca gcccgcgacc caggcccggc atgttggcgg cgatcaccac
     5821 ctgccagccc gcctggttga gctgggcgat ggcttccagt gcgcccggca gcggctccca
     5881 gtcggcgggc gactgcaggt agccttcgcg ttcgacgcag atggtgccgt cgcggtccag
     5941 gatgacgagc ttcatggcgg cgattatccg ccgccgcccg gcgtcagccg gccccagcgg
     6001 ccccaccagc gcccggcctg ccagtccagc cgggccggac ggccgggcgc aaacgcggtc
     6061 ggcggctggc cctcggccag caggcgctga ggcgcgccgg ccggggccag ctcgaaatac
     6121 acctcggtgc cgcccgggcc gcgtgcgctg gccggcacgt cgcgcagcag cagcacgcgc
     6181 acctcctgcg ccggcaacgg ccgacggccg gcgcggttca gctggctggc caccagcccg
     6241 gcgccgaccc ccagccagag cagcagccac agcgccagcg cccacggccg caggcgcgcc
     6301 cagccgtggc ggcgcagcgc cgcgcggccg gccaggcgca gccccagcac cgcgcccagc
     6361 agccccagcc cggtgaacac cgccggccgc gccgccgccc acagcgcctg cgccgggtcg
     6421 gacaccaggg tggccgggtg cagcgcctgc aggccgtcgg catcggcttc ggccagcgcc
     6481 tgcagcagcg cgcgcagcag cagcaccgcc agcggcggtg ccagcagcca ggtcaggccg
     6541 cgccagccga ggccggtggt ggcggcggcc gcaggccccg ggctcacgcc gccagccgcg
     6601 ccagctcggc cacgcggttc atggcctggt gcagggtctt gagcaggccc tggcggttca
     6661 gccgcaggtc ggcctgctcg gcgttgacca tgacgtggtc gaagaaggcg tccaccggcg
     6721 cacgcagcgc cgccagggac tgcaggctgg cggtgtagtc gccgcgcgcc aactgcgcgt
     6781 cggcctgcgg cagcacctgc tgcatggcgg cgtacagcgc ctgctcggcc ggctcctgca
     6841 gcagcagctc gctgacatgg gggtgcaccg ggctttcggc ctcggccttc ttcaggatgt
     6901 tgccgatgcg cttgttggcc gccgccaggg cgggtgcttc gggcagggcg gcaaaggcgc
     6961 gcaccgcctg caagtgcgga tgcacgtcgg ccaggcgcgg cgggcgcagg gccagcacgg
     7021 cgtcgacttc ctgcgcgctg aagccctgct cgcgcaggct gccggccagg cggtcgtaga
     7081 tgaacttgcc ggccagctcg tccttcacgc gcgacgggtt gaagtcggcc ttgccggcaa
     7141 aggtctgcgc cgccagctcg atcagctcgg gcagcgccag cggcaacgcc tgctcgatca
     7201 gcatgcggat tacgcccagc gcatggcggc gcagcgcgaa cgggtcgcgg tcgccggtgg
     7261 gcaggttgcc gatgccgaac atgccgacca gggtttccag cttgtcggcc agcgccacca
     7321 ccgtgccgac ctggccgcgc ggcagctcgt cgccggcaaa gcgcggcttg tagtggtctt
     7381 cgatggccag cgcgatgtcc tcgctcaggc cgtcgtggcg cgcgtagtag ccgcccatca
     7441 cgccctgcag ttcggggaac tcgcccacca tgtcggtcag cagatcggcc ttggccaggc
     7501 gcgcggcggt gtcggccgcc tgcgccagcg cggcgccgcc cagctgctgg ccgatctggc
     7561 gcgcgatggc gcgcacgcgc tcgacccgct cgccctgcgt gcccagctgg ttgtggtaga
     7621 ccacctgcgc cagctgcggc acacggctgt ccagcgattt cttgcggtcc tggtcgaaga
     7681 agaacttggc gtcggccagg cgcgggcgca ccacgcgctc gttgccggcg atcacggcgc
     7741 tcgggtcgtc cggcgtgatg ttgctgacca ccaggaagcg gtgcgtcagc cggccctgtg
     7801 cgtccagcag cggaaaatac ttctggttgg ccttcatggt caggatcagg cattcctgcg
     7861 gcacctgcag gaattcgggc tcgaacgcgc agaccagcac gttggggcgc tcgaccaggg
     7921 ccgtcacctc gtccagcaat gcttcatctt tgatagctgt cagcgcagtc cccgcctgtg
     7981 ttgcagccgc tttgatctgt gcttcgaggg tggcgcggcg ctcctcgaag ctggcgatca
     8041 ccgcgccctg ctggcgcagg tcgagggcgt agtcgtcggc gctggccagc tccaccgggt
     8101 cgctggcggc ctcgaagcgg tggccgtgcg tagcgcggcc ggcgcgcagg cccagcgccg
     8161 tgaccggcac cacggtttcg ccgtgcagcg ccaccaggcc gtgcgccggg cgcacgaact
     8221 gcacgctgtc ccagcccggg cgggcacagc cggcctgcag ctggtaggtc atgaccttgg
     8281 ggatcggcag gcgcgcgatg gcttcgtcca gcgccttctg caagccgctg tccagcgtgg
     8341 cgccggtgac cacgctgtcc aggaacagcg cctcggcctt gccgtccggc tggcgcttga
     8401 gctgcggcac caccgtgggc tcggcgccca gcgcctgcag cttcttgagc agcgccggcg
     8461 tggcctgacc ctgggcgtcc aggccgacgc tgaccggcat cagcttgagc tgcaccgcgc
     8521 ggtcggccgc cttggccgcc acatgcgtga tgtgcgccgc caggcggcgc gggctggcgt
     8581 agggcgtgac gcgcgagtcc gccgccgtca ggccctgcgc ctgcagctgc gccgccaggg
     8641 tggtggcaaa ggcctcgccc agctgccgca gggccttggg gggaagctcc tcgacgaaca
     8701 gctcgacaag caggttctgc gtggtcatat cggtcatgct catgcggcgg gtttttcgat
     8761 ctgctcgacc cacgcgcgcg gtgccagcgg aaagcccagg cgctggcggc tgtcgtaata
     8821 gctctgcgcc acggcgcgcg ccagggtgcg gatgcggccg atgtaggcgg cgcgctcggt
     8881 cacggaaatg gcgccgcgcg cgtccagcag gttgaaggtg tgggcggcct tgagcacctg
     8941 ctcataggcc ggcagcgcca gctgctggcc gatcaggtgc agcgcctgcc gctcgtgcgc
     9001 ggcaaaggcg gtgaacagga agtcggcgtc gctgtgctcg aagttgtagg tggactgctc
     9061 gacctcgttc tggtgataga cgtcgccata ggtcagcttg ctgccgtccg gcccttcggt
     9121 ccagaccagg ttgtagacgt tgtccacccc ctgcaggtac atggccaggc gctccaggcc
     9181 gtaggtgatc tcgccggtgg cgggccggca gtcgattccg ccgacctgct ggaagtaggt
     9241 gaactgggtc acttccatgc cgttgagcca cacctcccag cccaggcccc aggcgcccag
     9301 cgtggggttc tcccagtcgt cctcgacgaa gcggatgtcg ttcttcttca ggtcgaaccc
     9361 cagcgcctgc aggctgccca ggtacagctc caggatgttg gccggcgccg gcttgagcac
     9421 cacctggtac tggtagtagt gctgcaggcg gttggggttg tcgccgtagc ggccgtcctt
     9481 ggggcggcgg ctgggctgca cgtaggcggc gcgccagggc tcggggccga tggcgcgcaa
     9541 aaaagtggcg gtgtggctgg tgccggcgcc cacttccatg tcgtacggct gcagcagcgc
     9601 gcagccctga tcggcccagt actgctgtag cgacaggatg atttgctgaa aagtgagcat
     9661 ggcggtgcgt accggagcgg gcaaacccgg tattttagtg ttctgaatga aatgtggccc
     9721 cagcgcttga tgggcaagcg ccgatagcta taaaaaacgt agtaaactag ggccagcgcc
     9781 gcagcaccag gctggcgttg gtgccgccga agccgaagct gttggacagc acggtgctca
     9841 gccgggcgtc gcgcgtggcg gtcaccagcg gcatggcgcc gagctcgggg tcgggcgtct
     9901 cgacgttcac cgagccggcg atgaagccct tttgcagcat gatgaggcag tagatcgcct
     9961 cctgcacgcc ggtggcgccc agcgaatggc cggtgagcga cttggtgctg gaaaacggcg
    10021 gcacggcgtc gccgaacacc gcgcgcatgg cgcgcacttc ctgcatgtcg cccacgggcg
    10081 tggaggtgcc gtgggtgttg atgtagtcga tcggcgcatc cagcccgtcg atcgcctggc
    10141 gcatgcaggc aatcgccccg tcgcccgagg gcgccaccat gtcctcgccg tcgctggtgg
    10201 ccccgaagcc cacgacttcg gccaggatgg tggcaccgcg cgcctgggcg tgctccaggc
    10261 tttccagcac cacggcgccg ccgccgccgg cgatcacgaa gccgtcgcgg tgggcgtcgt
    10321 aggcgcgcga ggccttgtcg ggcgtggcgt tgtatttgct ggacatggcg cccatgccgt
    10381 cgaacagcat ggccatgccc caggaaagct cttcgccgcc gccggcgaac atcacgtcct
    10441 gcatgcccca ggcgatctgc tgcgccgccg cgccga
//
LOCUS       DAOQTZ010000092        10340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_7247_length_10340_cov_13.337190, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000092 DAOQTZ010000000
VERSION     DAOQTZ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7247_length_10340_cov_13.337190"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PKN51_10610"
     CDS             complement(<1..1097)
                     /locus_tag="PKN51_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT85594.1"
                     /translation="MRHSGRPTGPSHRSPTMMRPDPRQHQIALARQTVLAEGMELTEA
                     LVGAWLDRAWIVQSWRRCLARGARPEAPVVFDAVSPHARARSAEQHAALLAAARPELQ
                     RLAAQIAPIRYFALLTDAQGTVIDTAGAIDHRNRASHAIARVGVDLSEAAIGTSAIGA
                     ALAERQPVWLHRGEHFFADTGVYSCAGAPLFGPDGACLGMIDVTGVNAPERPELKHLV
                     AQSARQIEDALLLARPHQLRLHLAWPAGWQVSGAGSEGAALLCLDADGQVTGANSTAR
                     QMLPALHALHSGPLHASELFALPWASLFDRAAHGQPHTVPLWSGLRVQVRAERSTQPA
                     SAASPGARAAAPRSLKALETELIHQAVRDAGG"
     gene            1225..1692
                     /locus_tag="PKN51_10615"
     CDS             1225..1692
                     /locus_tag="PKN51_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNT85595.1"
                     /translation="MKVELKVNGKAVTADVPPNTLLVQLLREHLRLTGTHVGCDTGQC
                     GACTVIADGSAIKACNVLAAQMQGAEVTTIEGLAAADGTLHPMQAAFKECHGLQCGFC
                     TPGMVMSSVDLLARHPQASEAEIRALLDGNLCRCTGYQNIVKAVQTAQKAMAA"
     gene            1717..4089
                     /locus_tag="PKN51_10620"
     CDS             1717..4089
                     /locus_tag="PKN51_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNT85596.1"
                     /translation="MGANEFAKLPHIGESLRRKEDVRFLTGAGNYTDDVDQANQKYAV
                     FVRSPHAHARVKGVDTAAAAAMPGVAGVFSGKDIEGKMGGLPCGWLISNPDGSPMKEP
                     PHPILAIGKVRYVGDHVAMVVADTLQQAKDAAEAVQVDYEELAPVIDMRTARNGPALH
                     DEAPDNHCYKWTLGDKAAVDAAFAGAAHVTKLDITNNRLIPNAIEPRAANASYNRATD
                     EYVLYVANQNPHVERLLMTAFVLQLPEHKVRVVAPDVGGGFGSKIFLYAEDVALTWAA
                     KQLNCAIKWTAERSESFVSDAHGRDHITHAEMAMDKDGKFLAMRVHTDANLGAYLSTF
                     ASSVPTILYGTLLAGQYAAPQIYVEVDGWFTSTAPVDAYRGAGRPEATYVVERLVTRC
                     AWELGLSQDEIRKRNFITQFPYQTPVALQYDIGDYHACMNDAQKLADVAGFDARRQAS
                     EAKGLKRGIGYSSYIEACGLAPSNIAGALGARAGLFEAGEVRVHPTGSVTVFTGSHSH
                     GQGHETTFAQLVAARLGIDPSQVDIVHGDTGRVPFGMGTYGSRSLSVGGTAIMKALDK
                     IEAKAKKIAAHLMEASDADIDFANGEFTVKGTDKKIPFAQVALTAYVPHNYPLDKLEP
                     GLNETAFYDPTNFTFPAGTYICEVEVDPATGVVRVDRFTAVDDFGVIINPMIVEGQVH
                     GGLVQGMGQALMEHGVYDPDSGQLLTGSYMDYAMPRAHDFPEFRLGHVCTPCTHNPIG
                     SKGCGEAGAIGSPPAVINAVLDALKPLGVKDLDMPATPHRVWQAIQAAKA"
     gene            4100..4891
                     /locus_tag="PKN51_10625"
     CDS             4100..4891
                     /locus_tag="PKN51_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNT85597.1"
                     /translation="MYAFTYERPTSQADAARLAKAGGKLLAGGQTLLGSMKLRLASPE
                     QVVDLGAVKDLAGVRKDGNALVIGAMTRHADVAASADVRATIPALAQLASGIGDRQVR
                     AMGTLGGSVANNDPAADYPAAVLGLGATVVTSTRQIAADDYFQGLFATALDEGELITA
                     IRFPQPRRAAYMKFRQPASLFALIGVFVAQTDAGVRVAVTGGGNGVFRHAGLEQALSR
                     NFSPEAVAGVTIDAGELNGDLHGSAEYRAHLIGVMTQRAVAKALA"
     gene            5128..6051
                     /locus_tag="PKN51_10630"
     CDS             5128..6051
                     /locus_tag="PKN51_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT85598.1"
                     /translation="MSPTPASTASDPLSSIDALIAALQTAGYFADRRLATAVFLALRL
                     QRPLLLEGEPGVGKTELAKALAQVLGRELLRLQCYDGLEQREALYEWNYAAQLLHLRA
                     AEGRGTVEEAEREVYQPHYLIRRPLLQALQAPAPGAVLLIDEVDRADEPFEAFLLEYL
                     GEYQVSIPELGTVRAVATPLTVLTSNRTRELNDAVKRRCLYHWLDYPERERELAIVRA
                     RVPEAPAALAEQVAGFVHRLRSAPYAGHFQRAPGIAESVEWAKALVALDALLLDPEVV
                     QSTAGVLFKQREDVAALSAELITEALSPEAA"
     gene            6048..7277
                     /locus_tag="PKN51_10635"
     CDS             6048..7277
                     /locus_tag="PKN51_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT85599.1"
                     /translation="MTRVAPDAPARRSLLGDARRGKLADNVVAFARALRRAGVPLDAA
                     RIALAQQALQCVGVERRGDVGAALEAVLVSCEQDRAVFRELFDAFFRDPDLAHKLLAQ
                     MLPRAEAPAAPPPQRPRVREALQPPRPPGAAPRTQPDQSVDLDAAMTASERVRLRQAD
                     FNQLSASEYTLVERLVRDIALAVPEMRARRTRAGWRGPRVHWGRTLQGAAHSGGEVMA
                     LHRLQRRRQPLPLLLLVDVSGSMERYARLLLAFLHAATRQVQGQRVRRDVFTFGTRLT
                     DLTAAFRCSDTDAMLQQAGGAIEDFAGGTRLGDSLAVLRQRHARRLVGRRTLVLIVSD
                     GLDTGEPDVLARELAWLRRHTRRLLWLNPLLRFDGYTPSARGAAVLHRACDGMLAVHN
                     ISRLHDLARGIAQILKT"
     gene            7296..7868
                     /locus_tag="PKN51_10640"
     CDS             7296..7868
                     /locus_tag="PKN51_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HNT85600.1"
                     /translation="MEMQGSKALAITQQQAWDALNDPEVLKLCIPGCDSIEATGEGAY
                     ALVNAIKVGPVSAKFKGSLQLADVQAPDSYTIHFEGSGGAAGFGKGSAQVTLAPNATG
                     CELGYTVNATVGGKIAQVGQRLIDGVAKSMAESFFKRFEEEMQRRHPAAEAAVPAAPA
                     AAAADGAGVPLWVWILVAIVAILGVAWALR"
     gene            7897..8937
                     /locus_tag="PKN51_10645"
     CDS             7897..8937
                     /locus_tag="PKN51_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNT85601.1"
                     /translation="MENLDVTVLRALRDWRTAGRQALLVTVVRTWGSSPRPIGSIMAL
                     CEDGAVMGSVSGGCIEDDLIARYAHPGGADALPRSGPPQFTKYGITADEAHRFGLPCG
                     GTLELLLEFDPDGAALSELVRGLDSGRLMHRRVRLADGRVRLEPADKPTELVLTDTEL
                     VNTFGPEYRMLIIGAGQMSEYLATMALFCGFAVTVCDPRDEYRSSWSVPGVRVVASMP
                     DDTVAEMQPDARTCVVALTHDPKLDDLALLQALDTPAFYIGAIGSRRNNAARHQRLIE
                     HMGQTEASLKHLRGPIGIYIGSKTPPEIAVSVMAEVLAVKNGAPLPRDLDVAYVKDQQ
                     GQTAAGSACLLP"
     gene            8934..9644
                     /locus_tag="PKN51_10650"
     CDS             8934..9644
                     /locus_tag="PKN51_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT85602.1"
                     /translation="MNRPPPPDHPPATEPIAAIVMAAGAGSRFGFRPKGLLQRDGEAL
                     LARQLRLLAQAGVSRRVVVLGHHAGDITALLRKLRAQPAQAGLSWVVNPAPDDGPGSS
                     LRCGLASLPEDLDGVLVLLGDQPLLEPQDLRAVLQAWAERAPGIELVLPLHAGQPGHP
                     LVFGPQARQAVMAAQGGAGLREWRRAHPEQVQALPVDHPRCTTDIDTEADLSTLAAAH
                     GVHLRWPAGPARDQHRNQ"
     gene            9637..>10340
                     /locus_tag="PKN51_10655"
     CDS             9637..>10340
                     /locus_tag="PKN51_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85603.1"
                     /translation="MSDRRPGAAPAAAPPGRRGAARRWRAGAGRPRRCRRARPAPAQR
                     GCAAPGRSGVRTDGFSWQEHDSIRRPEFRTRIIPSPMSNAPPRLTIDFPDSLPVSARR
                     AEIEAALRAHQVVIVCGETGSGKTTQLPKIALAMGRGRLNARPGERGKLIGHTQPRRI
                     AATSVAKRIAEELRTPLGEVVGYKVRFGDRLTPGASVKLMTDGILLAETQTDPLLNAY
                     DTLIIDEAHERSLNID"
BASE COUNT         1580 a         3712 c         3673 g         1375 t
ORIGIN      
        1 ccgccggcgt cgcgcacggc ctggtggatc agctcggttt ccagcgcttt cagtgaacgt
       61 ggcgcggcgg cacgcgcgcc ggggctggcg gcgctggcgg gctgagtgct gcgctcggcc
      121 cgcacctgca cacgcaggcc ggaccacagg ggcacagtgt gcggctggcc gtgcgcggcc
      181 cggtcgaaca ggctggccca gggcagggcg aacagttcgc tggcgtgcaa cgggccgctg
      241 tgcagggcgt gcagggcggg cagcatctgg cgggcggtgc tgttggcgcc ggtgacttgg
      301 ccatcggcgt ccaggcacag cagggcggcg ccctcgctgc cggcgccgct gacctgccag
      361 ccggcgggcc aggccaggtg caggcgaagt tgatgcgggc gcgccagcag cagggcgtct
      421 tcgatctggc gggcggattg ggccaccagg tgcttgagtt cggggcgctc gggcgcgttg
      481 acgccggtga cgtcaatcat gcccaggcag gcgccgtcgg ggccgaacag gggcgcgccg
      541 gcgcagctgt agacgccggt gtcggcaaaa aaatgttcgc cgcggtgcag ccacacaggc
      601 tggcgctcgg ccagcgctgc gccgatggcg ctggtgccga tggccgcttc cgacaggtcc
      661 acgcccacgc gcgcgatggc gtggctggcg cggttgcggt ggtcgatggc gcctgcggtg
      721 tcgatgacgg tgccctgcgc gtcggtcagc agggcgaaat aacggatggg cgcgatctgc
      781 gcggccaggc gctgcagctc ggggcgggcg gcggccagca gggcggcgtg ctgctcggcg
      841 ctgcgggcgc gggcgtgggg cgagacggcg tcgaacacca ccggcgcctc gggccgcgcg
      901 ccacgcgcca ggcagcggcg ccagctttgc acgatccagg cgcggtcgag ccaggcgccg
      961 acgagcgcct cggtcagctc catgccctcg gccagcacgg tctggcgcgc cagggcgatc
     1021 tggtgttggc gggggtcggg gcgcatcatg gtgggcgagc ggtgcgacgg gccggtgggt
     1081 cggcccgagt gtctcattct gagattattc gagccgcttc cggccaaccg agggtatttc
     1141 ctaggtgcag cggcgcggct tgttccaaga atgaccgctt tcagtgcaaa ccgacaaacc
     1201 ccacgacaaa aggagacgcc cacgatgaaa gtcgagctca aggtcaatgg caaggcggtc
     1261 acggccgacg tgccgcccaa cacgctgctg gtgcagctgc tacgtgaaca cctgcgcctg
     1321 accggcaccc acgtgggctg cgacaccggc cagtgcggcg cctgcacggt gatcgccgac
     1381 ggcagcgcca tcaaggcctg caacgtgctg gcggcgcaga tgcagggcgc cgaggtgacc
     1441 accatcgaag gtctggcggc ggccgacggc acgctgcacc cgatgcaggc ggccttcaag
     1501 gaatgccacg gcctgcaatg cggtttctgc acgccgggca tggtgatgag cagcgtcgat
     1561 ctgctggcgc gccacccgca ggccagcgag gccgagatcc gcgccctgct ggacggcaac
     1621 ctgtgccgct gcacgggtta ccagaacatc gtcaaggcgg tgcagaccgc ccagaaggcc
     1681 atggccgcct gacggcccca ccgaggagac aagaccatgg gtgccaatga atttgccaaa
     1741 ctgccgcaca tcggcgaatc gctgaggcgc aaggaagacg tgcgctttct gaccggtgcc
     1801 ggcaactaca ccgacgacgt cgaccaggcc aaccagaaat acgccgtctt cgtgcgctcg
     1861 ccgcacgccc atgcccgcgt gaagggcgtc gacaccgccg ccgccgccgc catgcccggt
     1921 gtggccggcg tcttcagcgg caaggacatc gagggcaaga tgggcgggct gccctgcggc
     1981 tggctcatca gcaaccccga cggctcgccc atgaaggagc cgccacaccc catcctggcc
     2041 atcggcaagg tgcgctacgt gggcgaccac gtggccatgg tggtggccga tacgctgcag
     2101 caggccaagg acgccgccga ggcggtgcag gtcgactacg aagagctggc gccggtgatc
     2161 gacatgcgca ccgcccgcaa cggcccggcg ctgcacgacg aggcgcccga caaccactgc
     2221 tacaagtgga cgctgggcga caaggccgcg gtcgacgccg cctttgccgg cgccgcccac
     2281 gtgaccaagc tggacatcac caacaaccgg ctgatcccca acgccatcga gccgcgcgcc
     2341 gccaacgcca gctacaaccg cgccaccgac gaatacgtgc tgtacgtggc caaccagaac
     2401 ccgcacgtcg agcgcctgct gatgaccgcc ttcgtgctgc aactgcccga gcacaaggtg
     2461 cgcgtggtgg cgcccgacgt cggcggcggt tttggctcca agatctttct gtacgccgag
     2521 gacgtggcgc tcacctgggc ggccaagcag ctcaactgcg ccatcaagtg gacggccgag
     2581 cgcagcgaat cgtttgtgag cgacgcgcac gggcgcgacc acatcaccca cgcggaaatg
     2641 gcgatggaca aggacggcaa gttcctggcc atgcgcgtgc acaccgacgc caacctgggc
     2701 gcctacctgt ccaccttcgc ctccagcgtg ccgaccatcc tgtacggcac cctgctggcg
     2761 gggcagtacg cggcgccgca gatctacgtc gaggtggacg gctggttcac cagcaccgcc
     2821 ccggtcgatg cctaccgcgg cgccggccgg cccgaggcca cctacgtggt cgagcgcctg
     2881 gtcacgcgct gcgcctggga gctgggcctg tcgcaggacg agatccgcaa gcgcaacttc
     2941 atcacccagt tcccctacca gacgccggtg gcgctgcagt acgacatcgg cgactaccac
     3001 gcctgcatga acgacgcgca aaagctcgcc gacgtggccg gcttcgacgc ccgccgccag
     3061 gccagcgagg ccaagggcct gaagcgcggc atcggctaca gcagctacat cgaggcctgc
     3121 gggctggcgc ccagcaacat cgccggcgcc ctgggcgcgc gcgccggcct gttcgaggcc
     3181 ggcgaagtgc gcgtgcaccc gaccggcagc gtgacggtgt tcaccggctc gcacagccac
     3241 ggccaggggc acgaaaccac ctttgcccag ctggtggcgg cgcgcttggg catagaccca
     3301 tcgcaggtcg atatcgtgca cggtgacacc ggccgcgtgc ctttcggcat gggcacctac
     3361 ggcagccgct cgctgtcggt gggtggcacg gccatcatga aggcgctgga caagatcgag
     3421 gccaaggcca agaagatcgc cgcgcacctg atggaagcgt cggacgccga catcgacttt
     3481 gccaacggcg aattcaccgt caagggcacc gacaagaaga tcccctttgc ccaggtggcg
     3541 ctgacggcct acgtgccgca caactacccg ctcgacaagc tggagccggg cctgaacgaa
     3601 accgccttct acgaccccac caacttcacc ttcccggccg gcacctacat ctgcgaggtc
     3661 gaggtcgatc cggccaccgg cgtggtgcgg gtggaccgct tcaccgcggt ggacgacttc
     3721 ggcgtcatca tcaatccgat gatcgtcgaa ggccaggtgc acggcggtct ggtgcagggc
     3781 atgggccagg cgctgatgga gcacggcgtg tacgaccctg attcgggcca gctgctcacc
     3841 ggcagctaca tggactacgc catgccgcgc gcgcacgact tccccgagtt ccggctcggc
     3901 catgtctgca cgccctgcac gcacaacccg atcggcagca agggctgcgg cgaagcgggc
     3961 gccatcggct cgccgccggc ggtgatcaac gccgtgctgg acgcgctcaa acccctgggc
     4021 gtgaaggatc tggacatgcc cgccacgccg caccgcgtgt ggcaggcgat tcaagcggcc
     4081 aaagcctgag gagacacaca tgtacgcatt cacctacgag cgtcccacca gccaggccga
     4141 cgccgccagg ctggccaagg cgggcggcaa gctgctggcc ggcgggcaga ccctgctggg
     4201 atcgatgaaa ctgcgcctgg cttcccccga gcaggtggtg gacctgggcg ccgtcaagga
     4261 tctggccggc gtgcgcaagg acggcaacgc gctggtcatc ggcgccatga cgcgccacgc
     4321 cgacgtggcc gcgagcgccg acgtgcgcgc caccataccg gcgctggcgc agctggccag
     4381 cggcattggc gaccgccagg tgcgcgccat gggcacgctg ggcggatcgg tggccaacaa
     4441 cgacccggcg gccgactacc cggcggccgt gctcggcctg ggcgccaccg tggtcaccag
     4501 cacgcgccag atcgccgccg acgactactt ccagggcctg ttcgccaccg cgctggacga
     4561 gggcgagctg atcaccgcca ttcgctttcc gcagccgcgt cgcgcggcct acatgaagtt
     4621 ccgtcagccg gcctcgctgt tcgcgctgat cggcgtgttc gtggcgcaga ccgacgccgg
     4681 cgtgcgcgtg gccgtgaccg gcggcggcaa cggcgtgttc cgccacgccg gactggagca
     4741 ggcgctcagc cgcaacttca gccccgaggc ggtggccggc gtcacgatcg atgccggcga
     4801 gctcaacggc gacctgcacg gcagcgccga ataccgcgcg cacttgatcg gcgtcatgac
     4861 gcagcgcgcc gtcgccaagg cgctggcctg accctgggtg cggctgcggc gcctggcgcg
     4921 gtcaggcgga aggcggcgac gccgggcggg cggcagcacg gcagctgctg ccgccggtcg
     4981 ccagccgcgc ccctgtgcga acaggcctcg ccccggggcc gcagcggcgc gccggacttc
     5041 atgaataaaa agttgctccg gcgcgcatcc agcaagcgca agcagctatc gaattcgtag
     5101 tatcttccat tcaccttcgc aagcttcatg tcacccaccc cggcgtccac cgcgtccgac
     5161 ccgctgtcgt ccatcgacgc actcatcgcc gcgctgcaga ccgcgggcta ctttgccgac
     5221 cggcgcctgg ccacggcggt gtttctggcg ctcaggctcc agcgcccgct gttgctcgaa
     5281 ggcgagccgg gcgtgggcaa gaccgagctg gccaaggccc tggcccaggt gctggggcgc
     5341 gagctgctgc gcctgcagtg ctacgacggc ctggagcagc gcgaagccct gtacgaatgg
     5401 aactacgccg cgcaactgct gcacctgcgt gcggcggaag ggcgcggcac ggtggaggag
     5461 gccgagcgcg aggtctacca gccccactac ctgatccgcc gcccgctgct gcaggcgctg
     5521 caggcaccgg cgcccggtgc cgtgctgctg atcgacgagg tcgatcgcgc cgacgagccg
     5581 ttcgaagcct tcctgctcga atacctgggc gagtaccagg tcagcatccc cgaactcggc
     5641 accgtgcgcg ccgtcgccac gccgctgacc gtgctcacca gcaaccgcac gcgcgagctc
     5701 aacgacgccg tcaagcgccg ctgcctgtac cactggctcg actaccccga gcgcgaacgt
     5761 gagctggcca tcgtgcgcgc ccgcgtgccc gaggcaccgg cggcgctggc cgaacaggtg
     5821 gccggcttcg tgcaccggct gcgcagcgcc ccctacgcgg gccacttcca gcgcgccccc
     5881 ggcatcgccg aaagcgtgga atgggccaag gcgctggtgg cgctggacgc gctgctgctc
     5941 gatcccgagg tggtgcagag caccgccggc gtgctgttca agcagcgcga ggacgtggcg
     6001 gcgctgtctg ccgagctgat caccgaggcg ctgtcgccgg aggccgcgtg acccgcgtgg
     6061 cgccggacgc gccggcccgc cgttcgctgc tgggcgacgc gcgccgcggc aagctggccg
     6121 acaacgtggt ggcctttgcg cgcgcgctgc gccgcgccgg cgtgccactc gacgccgcgc
     6181 gcatcgcgct ggcgcagcag gcgctgcaat gcgtgggggt ggagcggcgc ggcgacgtag
     6241 gtgcggcgct ggaggccgtg ctggtcagct gcgagcagga ccgcgccgtg ttccgcgagc
     6301 tgttcgacgc cttctttcgt gaccccgacc tggcccacaa gctgctggcc cagatgctgc
     6361 cgcgcgccga ggcgccggcg gcgccgccgc cccagcgccc gcgcgtgcgc gaggccctgc
     6421 agccgcccag gccgccaggg gcggcgccgc gcacccagcc cgaccagagc gtcgatctcg
     6481 acgccgctat gacggccagc gagcgggtgc gcctgcggca ggccgacttc aaccagctca
     6541 gcgccagcga atacaccctg gtcgaacggc tggtgcgcga catcgcactg gccgtgcccg
     6601 agatgcgcgc ccggcgcacg cgcgccggct ggcgcggtcc gcgcgtgcac tggggccgca
     6661 cgctgcaggg cgccgcccac agcggcggcg aagtgatggc gctgcatcgc ctgcagcgcc
     6721 gccgccagcc gctgccgctg ctgctgctgg tcgacgtgtc gggttcgatg gagcgctacg
     6781 cgcgcctgct gctggccttt ctgcacgccg ccacgcgcca ggtgcagggg cagcgcgtgc
     6841 ggcgcgacgt gttcaccttc ggcacccggt tgaccgacct gacggcggcc ttccggtgca
     6901 gcgacaccga cgccatgctg cagcaggctg gcggcgccat cgaggacttc gccggcggca
     6961 cgcgcctggg cgattcgctg gccgtgctgc gccagcgcca tgcgcggcgc ctggtcgggc
     7021 ggcgcacctt ggtgctgatc gtcagcgatg gcctggacac cggcgagccg gacgtgctgg
     7081 cgcgcgagct ggcctggctc aggcgccaca cgcgccgcct gctgtggctc aacccgctgc
     7141 tgcgcttcga cggctacacc cccagcgcgc gcggtgccgc cgtgctgcac cgcgcctgcg
     7201 acggcatgct ggcggtgcac aacatcagcc gcctgcacga tctggcgcgc ggcatcgccc
     7261 agatcctgaa gacttgaaag gaaaggaccc accccatgga aatgcaaggc agcaaggccc
     7321 tggccatcac ccagcaacag gcctgggacg cgctcaacga ccccgaggtg ctgaaactgt
     7381 gcatccccgg ctgcgacagc atcgaggcca ccggcgaggg cgcctatgcg ctggtcaacg
     7441 ctatcaaggt cggcccggtg tcggccaagt tcaagggctc gctgcaactg gccgatgtgc
     7501 aggcgcccga cagctacacc atccatttcg aaggcagcgg cggtgccgcc ggcttcggca
     7561 agggcagcgc ccaggtcacc ctggcgccca acgccaccgg ctgcgagctt ggctacaccg
     7621 tcaacgccac cgtcggcggc aagatcgccc aggtcggcca gcggctgatc gacggcgtgg
     7681 ccaagtcgat ggccgagagc ttcttcaagc gcttcgaaga ggagatgcag cgccgccacc
     7741 cggcggccga ggcggcggtg cccgccgcgc ccgcggcggc ggcggccgac ggcgccggcg
     7801 tgccgctgtg ggtctggatt ctggtggcga ttgtggccat actgggtgtg gcctgggcgc
     7861 tgcgctgacc gggtcactgg ccacaggagg tgtcgcatgg aaaacctgga tgtcaccgtg
     7921 ctgcgcgccc tgcgcgactg gcggacggcg ggccggcagg ccctgctggt caccgtggtg
     7981 cgcacctggg gctcgtcgcc acggccgatc ggctcgatca tggccttgtg cgaggacggc
     8041 gcggtcatgg gctcggtctc gggcggctgc atcgaggacg acctgatcgc gcgctacgcc
     8101 cacccgggcg gcgccgacgc gctgccgcgc agcggcccgc cgcagttcac caagtacggc
     8161 atcaccgcgg acgaagcgca ccgcttcggt ctgccgtgcg gtggcacgct ggagctgctg
     8221 ctggagttcg accccgatgg cgcggcgctg agcgagctgg tccgtggcct ggacagcggc
     8281 cgcctgatgc accgccgggt gcgcctggcc gacggcaggg tgaggctgga gccggccgac
     8341 aagccgaccg aactggtgct gaccgacacc gagctggtca acaccttcgg ccccgagtac
     8401 cgcatgctga tcatcggcgc cggccagatg agcgagtacc tggccaccat ggcgctgttc
     8461 tgcggctttg ccgtcaccgt gtgcgacccg cgcgacgaat accgcagcag ctggagcgtg
     8521 cccggcgtgc gcgtggtggc cagcatgccc gacgacaccg tggccgagat gcagcccgac
     8581 gcgcgcacct gcgtggtggc gctcacgcac gaccccaagc tggacgatct ggcgctgctg
     8641 caggcgctgg acacgccggc gttctacatc ggcgccatcg gcagccggcg caacaacgcc
     8701 gcccgccacc agcggctgat cgagcacatg gggcagaccg aggcctcgct caagcacctg
     8761 cgcggcccga tcggcatcta catcggcagc aagacgccgc ccgagatcgc cgtcagcgtg
     8821 atggccgagg tgctggccgt gaagaacggc gcgccgctgc cgcgcgacct ggacgtggcc
     8881 tacgtcaagg accagcaggg ccagacggcg gccggctcgg cctgcctgct tccgtgaacc
     8941 gaccgccgcc gccggatcac ccgcctgcta ccgaaccgat agctgccatc gtcatggcgg
     9001 cgggcgccgg aagccgtttt ggcttcaggc ccaagggcct gctgcaacgt gacggcgaag
     9061 cgctgctggc gcgtcagctg cgcctgctgg cgcaggccgg ggtgtcgcgt cgggtggtcg
     9121 tgctgggtca tcacgcgggc gacatcacgg ccctgctgcg gaagctgcgc gcacagccgg
     9181 cgcaggccgg gctgagctgg gtcgtcaacc ccgcgcccga cgacggcccc ggttcttccc
     9241 tgcgctgcgg cctggccagc ctgcccgaag atctggacgg cgtgctggtg ctgctgggcg
     9301 atcagccgct gctggagccg caggacctgc gcgccgtgct gcaggcctgg gccgagcggg
     9361 cgcccggcat cgagctggtg ctgccgctgc acgccggcca gccgggccat ccgctggtgt
     9421 tcggcccgca ggcgcgccag gccgtgatgg cggcgcaagg cggcgccggc ctgcgcgaat
     9481 ggcgccgcgc gcaccccgag caggtgcagg cgctgcccgt cgatcaccca cgctgcacca
     9541 ccgacatcga caccgaggcc gacctctcca cgctggcggc cgcgcacggg gtgcacctgc
     9601 gctggcccgc cggccccgcg cgcgatcagc acaggaatca gtgaccgtcg ccccggcgca
     9661 gcgcctgctg cagcgccacc cggtcggcgc ggcgctgcgc gccggtggcg cgctggtgcc
     9721 ggccggccgc gccgctgccg gcgcgcgcgg ccagcgccag cgcaacgtgg ttgcgcggcg
     9781 ccggggcggt ctggggtgcg gacggacggg ttttcatggc aggaacatga ctccatccgg
     9841 cgcccggagt tccgcacgcg gataatcccc agccccatga gcaacgcgcc accccgcctg
     9901 accatcgatt tccccgacag cctgccggtg tcggcgcgcc gcgccgagat cgaggccgcg
     9961 ctgcgcgcgc accaggtggt catcgtgtgc ggcgaaaccg gctcgggcaa gaccacgcag
    10021 ctgcccaaga tcgccctggc catggggcgc gggcggctga acgcgcggcc gggcgagcgc
    10081 ggcaagctga tcggtcacac ccagccgcgc cgcatcgccg ccaccagtgt cgccaagcgc
    10141 attgccgaag aactgcgcac gccgctgggc gaggtggtgg gttacaaggt gcgctttggc
    10201 gaccgcctga cgccgggcgc cagcgtcaag ctgatgaccg acggcattct gctggccgag
    10261 acgcagaccg acccgctgct caacgcctac gacacgctga tcatcgacga ggcgcacgag
    10321 cgcagcctga acatcgactt
//
LOCUS       DAOQTZ010000093        10241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_7341_length_10241_cov_15.557334, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000093 DAOQTZ010000000
VERSION     DAOQTZ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7341_length_10241_cov_15.557334"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKN51_10660"
     CDS             <1..278
                     /locus_tag="PKN51_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85604.1"
                     /translation="SLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASYTLQEMLTVKS
                     DDVQGRTKVYESIVKGEHAIDAGMPESFNVLVKEIRSLGLDIELERS"
     gene            302..4534
                     /gene="rpoC"
                     /locus_tag="PKN51_10665"
     CDS             302..4534
                     /gene="rpoC"
                     /locus_tag="PKN51_10665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNT85605.1"
                     /translation="MKSLLDLFKQFTPDEHFDAIRIGLASPEKIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIDLAAPCAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGMTPLKKFSIM
                     SEDDYDAKRKEYGDEFVARMGAEGIKELLESIDLDTEIERLRGDLTGSEVKVKKNAKR
                     LKVLEAFKKSGIKPEWMVLNVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNSR
                     LRRLLELKAPEIIARNEKRMLQEAVDSLLDNGRRGKAMTGANKRALKSLADMIKGKSG
                     RFRQNLLGKRVDYSGRSVITVGPTLMLHQCGLPKLMALELFKPFIFARLEQMGIATTI
                     KAAKKEVEAGTPVVWDILEEVITEHPVLLNRAPTLHRLGIQAFEPILIEGKAIQLHPL
                     VCAAFNADFDGDQMAVHVPLSVEAQMEARTLMLASNNVLFPASGEPSIVPSQDVVLGL
                     YYTTRERINGKGEGMVFADIGEIERALAAGEVELTAKVSVRLTEWSKDKATGELTPST
                     SLVETTAGRALLSEILPKGLPFSLMNKALKKKEISRLINASFRKCGLKETVVFADKLL
                     QNGFRLATRAGISIAIEDMLVPGEKAGIIERAEREVKEIEQQYVSGLVTAGERYNKVV
                     DIWGKAGDEVSKVMMSQLAKQKTLDRHGKEVEQESFNSIYMMADSGARGSAAQIRQLA
                     GMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRR
                     LVDVTQDLVVTEEDCGTSDGSLMRAIVEGGEVVESLRERILGRTAAEDLVNPETQEVV
                     VQAGQMLDEDAVDELEGAGIDEVRVRTALTCGTRFGICAKCYGRDLGRGGLVNVGEAV
                     GVIAAQSIGEPGTQLTMRTFHIGGAASRAAVASNVEAKTAGNIGFNPTMRYVTNTKGE
                     LVVISRSGEITIHDEHGRERERHKVPYGAILTVKADQAIKAGAILANWDPLTRPIITE
                     YAGTVKFENVEEGLTVAKQVDEVTGLSTLVVIDPKRRGSAKIVRPQVKLVDAGGNEVK
                     IPGTDHSVTIGFQVGALIQVRDGQDVGPGEVLARIPVEGQKTRDITGGLPRVAELFEA
                     RSPKDKGVLAEQTGTISFGKETKGKIRLQITDPDGKVWEELIPKEKNLLVHEGQVVNK
                     GESIVDGPADPQDILRLLGIDELSRYIVDEVQDVYRLQGVKINDKHIEVIVRQMLRRV
                     VVDNPGDSGYIAGEQVERSEMLDTNDALRAEGKLPATYTNLLLGITKASLSTDSFISA
                     ASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGSGLAYHQARKAKDQMDEAER
                     RAIAEAEAAELAGATEMTEAGESANLDGAAAE"
     gene            4611..5189
                     /locus_tag="PKN51_10670"
     CDS             4611..5189
                     /locus_tag="PKN51_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNT85606.1"
                     /translation="MWVWGVWIAAALLLFWGIGAYNRLVRLRAAVTRAFAALDEQLVR
                     QLVWIQGGLPEALRDGPHTTPGELNDEVTAAWGRLHAASEQLAVALAQARAQPLDVAA
                     MAALVMSHEALRTAWAHALADAVPPDAVPSADRLQARWMRLLHQSLPLRAAFNDAAQS
                     YNQAIRQFPASLLAHLFGFQPAGTVTRLAEGR"
     gene            5189..6517
                     /gene="rsmB"
                     /locus_tag="PKN51_10675"
     CDS             5189..6517
                     /gene="rsmB"
                     /locus_tag="PKN51_10675"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNT85607.1"
                     /translation="MPSAPTQTLPLWRQLQACARLVAGVRAGRSLTAELAGVDALLRP
                     GAQALSFHVLRWLGLAQALRAQLARRPPPPAVDALLCTSLALAQPADGAPYEAFTLLD
                     QSVEAAKRDRTLRPHAAFINACLRRYLRERAALTAAVQHDPVARWNHPRWWAERLQRD
                     HPQHWQAILAAAQQAAPMDLRVNVQRIGVADYGARLAQQGIGADALGGAALRLHRPRT
                     VQALPGFADGQVSVQSATAQRAAPLLLQGCLDVDAPRILDACAAPGGKTAHLLELRPD
                     ARVTALELDPQRGARISDTLARLGLHAEVRVADAADLPSWWRGERYDAILLDAPCSAS
                     GIVSRHPDVRWLRREADLAQLSAQQDRLLQALWPLLRPGGHLLYCTCSVFAQEGRERV
                     QAFLARHSDAIPCPAPGHILPCAGEAAAASGDNAGCDDGFYYALLRKRLD"
     gene            6471..7097
                     /locus_tag="PKN51_10680"
     CDS             6471..7097
                     /locus_tag="PKN51_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNT85608.1"
                     /translation="MTTGFITRCCASVWISRLLLAWALACGLGAVARAEGAALSALSV
                     AREGNELILSARLDFALSPVVEDALHKGIPVHFVAEAEVLRERWYWADRSVALARRYL
                     RLSYQPLTRRWRLNTSSEPLTNAGLGVSLTQSFDTLDEALTAVQRIGRWRIASAADID
                     DGGRHLLRFRFRLDASQLPRTLQIGSVGHSDWVISVERRIDLTQELAQ"
     gene            7094..9400
                     /locus_tag="PKN51_10685"
     CDS             7094..9400
                     /locus_tag="PKN51_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT85609.1"
                     /translation="MSTAAAVDPAQSARRRRSRWALWAAGTLLVGIGLVLLFLLAQAT
                     NNRLLYERYYTRLFIVNVVVAAVLGLVILWGVYRLLMRLRRGKFGSRLLIKLAAIFGL
                     VGVVPGLLIYVVSYQFVSRSIESWFDVRVEGALDSGLTLARTSLDTQAREFGNQLRTA
                     SAALADVPDALAALPLEQLRVQLGASDLTLWSASGRLLASAGRSRFQLTPERPSGAML
                     RNLRAQGVATQIEGLDDVVPGGTTPEARVRALVAVSARTIGLVGEQRLLQAVQPLPTS
                     LVTHALAVQEAHREYQERALGREGLRRMYIGTLTLSLFLAVFGAVLLAVALGKQLATP
                     LLMLADGMRQVATGDLTPKVILPGRDELVGLTRSFADMTQQLADARAALDASMHAVDA
                     SRARLQTILDNLTAGVIVLDAQGLIQSSNPGATRILRVPLAASAGRPLGSLEGLQAFA
                     AEVQTQFASFEGERGEHGLENWQKSFELYGHPGGGVATDALTLIARGAPLPDGLRLLV
                     FDDISEIVSAQRAHAWGEVARRLAHEIKNPLTPIQLSAERLEHKLADKLPEAERALLV
                     RAVRTIVDQVDAMKRLVNEFRDYARLPMADLKPVDLNALVTEVLQLYGGAGDMQHRQA
                     VPVHFESDPHCPPILGDAQQLRQVIHNLVQNAIDASEGVVRDGPQAQAQGVIVRTQWQ
                     DNLQRVRLAVIDSGHGFAEAILKRAFEPYVTTKPKGTGLGLAVVRKIADEHGARIDVS
                     NRINDGQILGAQVSLSFQPASEGRTALT"
     gene            9431..10138
                     /locus_tag="PKN51_10690"
     CDS             9431..10138
                     /locus_tag="PKN51_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT85610.1"
                     /translation="MANILVVDDELGIRDLLSEILNDEGHTVELAENAAQARAARQNG
                     RPDLVLLDIWMPDTDGVTLLKEWRGAGLLTMPVIMMSGHATIDTAVEATRIGAQSFLE
                     KPITLQKLLKTVDHALSRDVPARRAVAEVVEAGVVVHAPADLTVEAAMTGGQSLPAAL
                     DMSPQGQQTFDLDKPLRDARDAFEKAYFEFHLAKEGGSMTRVAEKTGLERTHLYRKLK
                     QLGVDLSRNRRGAAVAL"
     gene            10166..10241
                     /locus_tag="PKN51_10695"
     tRNA            10166..10241
                     /locus_tag="PKN51_10695"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10199..10201,aa:Phe,seq:gaa)
BASE COUNT         1707 a         3599 c         3397 g         1538 t
ORIGIN      
        1 actcgctggt gacgcagcag ccgctgggcg gcaaggcgca gttcggcggc cagcgcttcg
       61 gcgagatgga agtgtgggcg ctggaagcct acggcgcttc ctacacgctg caggaaatgc
      121 tcaccgtcaa gtccgacgac gtgcagggcc gcaccaaggt gtacgaatcc atcgtcaagg
      181 gcgaacacgc gatcgacgcc ggcatgcccg agtcgttcaa cgtgctggtc aaggaaatcc
      241 gctcgctcgg cctcgacatc gagctggagc gctcctgatt cattgaaaag gaaagagtca
      301 catgaaatcg ctgctcgacc tgttcaagca attcacgccg gatgagcact tcgatgccat
      361 ccgcatcggc ctggcctcgc ccgagaagat ccgttcctgg tccttcggcg aggtgaaaaa
      421 gcccgaaacc atcaactacc gcaccttcaa gcccgagcgc gacggcctgt tctgcgccaa
      481 gatcttcggg cccatcaagg actacgagtg cctgtgcggc aagtacaagc gcctcaagca
      541 tcgcggcgtg atctgcgaga agtgcggcgt cgaagtcacg cagaccaagg tgcgccgcga
      601 gcgcatgggc cacatcgacc tggccgcgcc ctgcgcccac atctggttcc tgaagtcgct
      661 gcccagccgt ctgggcatgg tgctcgacat gacgctgcgc gacatcgagc gcgtgctgta
      721 cttcgaagcc tacgtggtca ccgaccccgg catgacgccg ctgaagaagt tcagcatcat
      781 gagcgaggac gactacgacg ccaagcgcaa ggaatacggc gacgaattcg tcgcgcgcat
      841 gggcgccgaa ggcatcaagg agctgctcga gagcatcgac ctggataccg aaatcgagcg
      901 cctgcgcggt gacctgacgg gctccgaggt caaggtcaag aagaacgcca agcgcctgaa
      961 ggtgctggag gctttcaaga aatccggcat caagcccgag tggatggtgc tgaacgtgct
     1021 gccggtgctg ccgcccgatc tgcgtccgct ggtgccgctg gacggcgggc gtttcgccac
     1081 ctcggacctg aacgacctgt accgccgcgt catcaaccgc aactcgcgcc tgcgccgcct
     1141 gctggaactc aaggcgcccg agatcatcgc gcgcaacgaa aagcgcatgc tgcaggaagc
     1201 cgtcgacagc ctgctggaca acggccgccg tggcaaggcc atgaccggcg ccaacaagcg
     1261 cgccctcaag tcgctggccg acatgatcaa gggcaagagc ggccgcttcc gccagaacct
     1321 gctgggcaag cgtgtcgact attcgggccg ttcggtcatc accgtcggcc cgacgctgat
     1381 gctgcaccag tgcggcctgc ccaagctgat ggcgctggag ctgttcaagc ccttcatctt
     1441 cgcccgtctg gagcagatgg gcatcgccac caccatcaag gccgccaaga aagaggtcga
     1501 ggccggcacg ccggtcgtgt gggacatcct ggaagaagtc atcaccgagc atccggtgct
     1561 gctcaaccgc gcgcccacgc tgcaccgcct gggcatccag gccttcgagc cgatcctgat
     1621 cgaaggcaag gccattcagc tgcacccgct ggtgtgcgcc gccttcaacg ccgacttcga
     1681 cggtgaccag atggccgtgc acgtgccgct gtcggtggag gcgcagatgg aagctcgcac
     1741 cctgatgctg gcttccaaca acgtgctgtt cccggcctcg ggcgagccct ccatcgtgcc
     1801 ttcgcaggac gtggtgctgg gcctgtacta caccacgcgc gagcgcatca acggcaaggg
     1861 cgagggcatg gtcttcgccg acatcggcga gatcgagcgc gcgctggccg ccggcgaggt
     1921 cgagctcacc gccaaggtga gcgtgcgcct gaccgagtgg agcaaggaca aggccacggg
     1981 cgagctgacg ccatccacca gcctggtcga aaccacggcc ggtcgcgccc tgctgtccga
     2041 gatcctgccc aagggcctgc cgttctcgct catgaacaag gcgctcaaga agaaggaaat
     2101 ctcgcgcctc atcaacgcca gcttccgcaa gtgcggcctg aaggaaaccg tggtgtttgc
     2161 cgacaagctg ctgcagaatg gcttccgcct ggccacgcgt gccggcatct cgatcgccat
     2221 cgaggacatg ctggtaccgg gcgaaaaggc cggcatcatc gagcgcgccg agcgcgaggt
     2281 caaggaaatc gagcagcagt acgtctcggg cctggtcacc gccggcgagc gctacaacaa
     2341 ggtggtcgac atctggggca aggccggcga cgaagtgtcc aaggtcatga tgagccagct
     2401 ggccaagcag aagacgctgg accgccatgg caaggaagtc gagcaggagt ccttcaactc
     2461 catctacatg atggccgact cgggtgcccg cggcagcgcg gcccagatcc gtcagctcgc
     2521 cggcatgcgc ggcctgatgg ccaagccgga cggctcgatc atcgaaaccc ccatcacggc
     2581 caacttccgc gaggggttga acgtgctgca gtacttcatc tccacgcacg gcgcgcgcaa
     2641 gggtctggcc gacacggcgc tgaagaccgc caactccggt tatctgacgc gccgtctggt
     2701 cgacgtgacg caggacctgg tcgtcaccga ggaagactgc ggcaccagcg acggctcgct
     2761 gatgcgcgcc atcgtcgaag gcggtgaggt ggtcgaatcg ctgcgcgagc gcatcctggg
     2821 ccgcaccgcg gcagaggacc tggtcaatcc agagactcag gaagtggtgg tgcaggcggg
     2881 ccagatgctc gacgaagacg cggtcgacga actggaaggc gccggcatcg acgaggtgcg
     2941 cgtgcgcacc gccctgacct gcggcacccg tttcggcatc tgcgccaagt gctacggccg
     3001 cgacctgggc cgcggcggcc tggtcaacgt cggcgaggcg gtgggcgtga tcgccgcgca
     3061 gtcgatcggc gagcccggca cgcaactgac catgcgcacc ttccacatcg gtggtgcggc
     3121 gtcgcgcgcg gccgtggctt ccaacgtcga agccaagacc gccggcaaca tcggcttcaa
     3181 cccgaccatg cgctacgtca ccaacaccaa gggtgagctg gtggtgatct cgcgttcggg
     3241 cgaaatcacc atccacgacg agcacggccg cgaacgcgag cgccacaagg tgccctacgg
     3301 cgccatcctg accgtcaagg ccgaccaggc catcaaggcc ggcgccatcc tggccaactg
     3361 ggacccgctg acacgcccca tcatcaccga atacgccggt acggtgaagt tcgagaacgt
     3421 cgaggaaggc ctgacggtgg ccaagcaggt cgatgaagtg accggcctgt ccaccctggt
     3481 ggtgatcgat cccaagcgtc gcggttcggc caagatcgtg cggccgcagg tcaagctggt
     3541 cgacgccggc ggtaacgagg tcaagatccc cggtaccgac cactcggtga ccatcggctt
     3601 ccaggtcggc gcgctgatcc aggtgcgcga cggccaggac gtcggccccg gcgaagtgct
     3661 ggcgcgcatt ccggtcgaag gccagaagac gcgcgacatc accggcggtc tgccgcgcgt
     3721 ggccgagctg ttcgaagccc gttcgcccaa ggacaagggc gtgctggccg aacagaccgg
     3781 caccatctcg ttcggcaagg aaaccaaggg caagatccgc ctgcaaatca ccgaccccga
     3841 cggcaaggtc tgggaagagc tgattcccaa ggaaaagaac ctgctggtgc acgaaggcca
     3901 ggtggtcaac aagggcgagt ccatcgtcga cggtccggcc gacccgcagg acatcctgcg
     3961 cctgctgggc atcgacgagc tgtcgcgcta tatcgtcgac gaggtgcagg acgtctaccg
     4021 cctgcaaggc gtgaagatca acgacaagca catcgaggtc atcgtgcgcc agatgctgcg
     4081 ccgcgtggtg gtcgacaacc cgggcgactc gggctacatc gccggcgagc aggtcgagcg
     4141 cagcgaaatg ctggacacca acgatgccct gcgcgccgaa ggcaagctgc cggccaccta
     4201 caccaacctg ctgctgggca tcaccaaggc ctcgctctcc accgattcgt tcatctcggc
     4261 ggcttccttc caggaaacca cgcgcgtgct caccgaagcg gccatcatgg gcaagcgcga
     4321 cgagctgcgc ggtctgaagg aaaacgtcat cgtcggccgc ctgatcccgg cgggttccgg
     4381 cctggcctac caccaggcgc gcaaggccaa ggaccagatg gacgaggccg agcgccgcgc
     4441 catcgccgaa gccgaggcgg ccgagctggc cggcgccacc gaaatgaccg aagcgggcga
     4501 gtccgccaac ctggacggag ctgccgccga gtaagcgatt gctctttgtt tgatagcgcc
     4561 ctccgcatgc tgctaaagcg ctgagggcgc tttttcttgg ggagtgcggg atgtgggtgt
     4621 ggggcgtgtg gatcgcggcg gcgctgctgc tgttctgggg catcggtgcc tacaaccgcc
     4681 tggtgcgcct gcgtgccgcg gtgaccaggg cgtttgcggc gctggacgag cagctggtgc
     4741 ggcaactggt ctggatccag ggtggcctgc cggaagccct gcgcgatggt ccgcacacca
     4801 cgccgggtga actgaacgac gaggtcacgg ccgcctgggg ccgcctgcac gcggccagcg
     4861 agcaactcgc cgtggcgctg gcgcaggcgc gcgcgcagcc gctggacgtg gcggccatgg
     4921 cggccctggt gatgtcgcat gaagccttgc gcaccgcctg ggcgcacgcg ctggccgacg
     4981 ccgtgccgcc cgacgccgtg ccctcggccg accgcctgca ggcgcgctgg atgcgcctgc
     5041 tgcaccagtc gctgcccttg cgcgctgcct tcaacgacgc cgcgcagagc tacaaccagg
     5101 cgattcgcca gtttccggca tcgctgctgg cgcacctgtt cggttttcag ccggccggca
     5161 ccgtcacccg actggccgaa ggtcgctgat gccttccgcc cccacgcaaa ccctgcccct
     5221 gtggcgccag ttgcaggcct gtgcgcggct ggtggcgggc gtgcgggcgg ggcgttcgct
     5281 gacggccgaa ctggcgggcg tcgatgccct gctgcgcccc ggcgcccagg cgctgtcgtt
     5341 tcatgtgctg cgctggctcg ggctggcgca ggcgctgcgc gcccagctgg cgcgccgccc
     5401 cccaccgccc gccgtcgatg ccctgctgtg caccagcctg gcgctggcgc agccggccga
     5461 cggcgcgccc tacgaagcct tcaccctgct cgatcagtcc gtcgaagccg ccaagcgcga
     5521 tcgcaccctg cgcccgcacg ccgccttcat caacgcctgc ctgcggcgct acctgcgcga
     5581 gcgcgcggcc cttaccgcgg ccgtgcagca cgacccggtg gcgcgctgga accacccgcg
     5641 ctggtgggcc gagcggctgc agcgcgatca cccgcagcac tggcaggcca tcctggcggc
     5701 ggcacaacag gcggcgccga tggacctgcg cgtcaacgtc cagcgcatcg gcgtggccga
     5761 ctacggcgcg cgcctggccc agcagggcat cggtgccgac gcactgggcg gcgccgccct
     5821 gcgcctgcac cggccgcgca cggtgcaggc gctgccgggt ttcgccgacg gccaggtctc
     5881 ggtgcaatcg gccaccgccc agcgcgccgc gcccctgctg ttgcagggct gcctcgatgt
     5941 cgacgcgccg cgcatcctgg acgcctgtgc ggctcccggc ggcaagaccg cccacctgct
     6001 ggagctgcgc ccggacgccc gcgtcaccgc gctggagctc gatccgcagc gcggcgcccg
     6061 catcagcgat acgctggcgc gcctgggcct gcacgccgag gtgcgcgtgg ccgacgccgc
     6121 cgacctgccg tcctggtggc ggggcgagcg ctacgacgcc atcttgctcg acgccccctg
     6181 cagcgcctcc ggcatcgtca gccgtcaccc cgacgtgcgc tggctgcggc gcgaagcgga
     6241 ccttgcacag ctgtccgccc agcaggaccg tctgctgcag gcgctgtggc cgctgctgcg
     6301 gccaggcggc cacctgcttt actgcacctg ctcggtgttc gcccaggaag ggcgtgagcg
     6361 cgtgcaggcg tttcttgcac gccacagtga cgccatacca tgccccgcgc ccggccatat
     6421 attgccctgc gccggcgagg cggccgccgc cagtggtgac aatgcgggct gtgacgacgg
     6481 gttttattac gcgctgctgc gcaagcgtct ggattagccg cctgctgctg gcctgggcgc
     6541 tggcctgcgg cctgggcgcc gtcgcccgtg ccgagggcgc cgccctgagc gcgctcagcg
     6601 tcgcgcgcga gggtaacgag ctgatcctgt cggcccgcct cgactttgcc ctgtcaccgg
     6661 tggtcgaaga cgccctgcac aaaggcattc cggtgcactt cgtggccgag gccgaagtgc
     6721 tgcgcgagcg ctggtactgg gccgaccgca gcgtggccct ggcgcggcgc tacctgcgcc
     6781 tgtcctacca gcccctgacg cgccgctggc gcctgaacac ttcgtccgaa ccgctgacca
     6841 atgccggcct gggcgtcagc ctgacgcaga gcttcgacac cctggacgaa gccctcaccg
     6901 ccgtgcagcg cattggccgc tggcgcatcg ccagcgccgc cgacatcgac gacggcggcc
     6961 gccatctgct gcgctttcgc ttccggctcg acgccagcca gctgccgcgc accctgcaaa
     7021 tcggctccgt cgggcattcc gactgggtga tctcggtcga gcgccgcatc gacctgacgc
     7081 aggagctggc ccagtgagta ccgccgccgc cgtcgacccg gcccagagtg cgcgccgccg
     7141 ccgttcgcgc tgggcgctgt gggccgccgg caccctgctg gtgggcatcg gcctggtgct
     7201 gctgttcctg ctggcccagg ccaccaacaa ccgcttgctg tacgagcgct actacacacg
     7261 gctgttcatc gtcaacgtgg tcgtcgccgc cgtgctgggg ctggtcattc tgtggggggt
     7321 gtaccgcttg ctcatgcgcc tgcggcgcgg caagttcggc agccggctgc tgatcaagct
     7381 ggccgccatc ttcgggttgg tgggggtggt gccggggttg ctgatctacg tcgtgtccta
     7441 tcagttcgtg tcgcgctcga tcgaatcgtg gttcgacgtg cgggtcgaag gcgcgctgga
     7501 ttccggcctc acgctggcgc gcacctcgct ggatacccag gcgcgcgagt tcggcaacca
     7561 gttgcgcacc gcttcggccg cgctggcgga cgtgcccgat gcgctggcgg cgttgccgct
     7621 ggagcaactg cgcgtgcaac tgggcgccag cgacctgacc ctgtggagcg cgtcgggccg
     7681 tctgctggcc agcgccggac ggtcgcgctt tcagctcacg ccggagcgcc ccagtggcgc
     7741 catgctgcgc aacctgcggg cccagggcgt ggccacgcag atcgagggac tggacgacgt
     7801 cgtgcccggt ggcaccaccc ccgaagcccg cgtccgcgcc ctggtggcgg tcagtgcccg
     7861 caccatcggc ctggtgggcg agcagcgcct gctgcaggcg gtgcagccgc tgcccaccag
     7921 cctggtcacg cacgccctgg cggtgcagga agcgcatcgc gaataccagg aacgcgccct
     7981 gggccgcgaa ggcctgcggc gcatgtacat cggcaccctc accctgagcc tgtttctggc
     8041 cgtgttcggc gccgtgctgc tggccgtggc actgggcaag cagctggcca cgccgctgct
     8101 gatgctggcc gatggcatgc gccaggtggc caccggcgac ctgaccccga aggtgatcct
     8161 gcccgggcgc gacgaactgg tgggcctgac ccgctcgttc gccgacatga cccagcagct
     8221 ggccgacgcc cgcgccgccc tcgacgccag catgcacgcg gtggacgcct cgcgggcgcg
     8281 cctgcagacc atcctcgaca acctgaccgc cggcgtcatc gtgctcgatg cccagggcct
     8341 gatccagtcg tccaacccgg gcgccacgcg catcctgcgc gtgccgctgg ccgccagcgc
     8401 cggccgcccg ctgggcagcc tggagggcct gcaggccttt gccgccgaag tccagaccca
     8461 gttcgccagc tttgaaggcg agcgcggcga acatggcctg gaaaactggc agaagtcgtt
     8521 cgagctgtac ggccacccgg gcggcggcgt ggccaccgac gccctgaccc tgattgcccg
     8581 cggcgctccg ctgccggacg gcctgcggct gctggtgttc gacgacatct ccgaaatcgt
     8641 ctccgcccag cgtgcccacg cctggggcga agtggcgcgg cggctggcgc acgaaatcaa
     8701 gaacccgctt acccccatcc agctgtcggc cgagcggctg gagcacaagc tggccgacaa
     8761 actgcccgaa gccgagcgcg ccctgctggt gcgcgccgtg cgcaccatcg tcgatcaggt
     8821 cgacgccatg aagcgcctgg tcaacgaatt ccgcgactac gcccgcctgc ccatggccga
     8881 cctcaagccg gtcgacctca acgccctggt caccgaagtg ctgcagctgt atggcggcgc
     8941 cggcgacatg cagcaccgcc aggccgtgcc cgtgcacttc gagagcgacc cccactgccc
     9001 gcccatcctg ggcgacgccc agcagttgcg ccaggtcatc cacaacctgg tgcagaacgc
     9061 catcgacgcc agcgaaggcg tggtgcgtga cggcccgcaa gcccaggcgc aaggcgtgat
     9121 cgtgcgcacc cagtggcagg acaacctgca gcgcgtgcgg ctggcggtga tcgacagcgg
     9181 ccacggcttt gccgaggcca tcctcaagcg cgccttcgaa ccctacgtca ccaccaaacc
     9241 caagggcacc ggcctgggcc tggccgtggt gcgcaagatc gccgacgagc acggcgcccg
     9301 catcgatgta tccaatcgca tcaacgatgg ccagattctg ggggcgcaag tatcgttatc
     9361 attccagcca gcctccgaag ggcgcaccgc cctgacctag tcccgctccg cttcacgacc
     9421 cgcactgctt atggccaaca tcctggtggt tgacgacgaa ctcggcattc gggatctcct
     9481 gtccgagatc ctcaatgacg aagggcacac cgtcgaactg gccgagaacg cggcacaggc
     9541 acgcgccgca cgccagaacg gccgccccga tctggtgctg ctcgacatct ggatgcccga
     9601 caccgatggc gtcaccctgc tcaaggaatg gcgcggcgcc ggcctgctga ccatgccggt
     9661 catcatgatg agcggtcacg ccaccatcga caccgcggtc gaggccacgc gcatcggcgc
     9721 gcagtcgttt ctggaaaagc ccatcaccct gcagaagctg ctcaagaccg tcgatcatgc
     9781 gctgtcgcgc gacgtgccgg cccgccgcgc cgtggccgag gtggtcgagg ccggtgtcgt
     9841 cgtgcatgcc ccggcggacc tgacggtgga agccgccatg accggtggcc agagcctgcc
     9901 cgcggcccta gacatgagcc cgcagggcca gcagaccttc gatctcgaca aacccctgcg
     9961 cgacgcgcgc gacgccttcg aaaaagccta cttcgagttc catctggcca aggaaggcgg
    10021 ctccatgacc cgcgtggctg aaaaaaccgg gctggagcgc acgcacctgt accgcaagct
    10081 caagcagctg ggggtcgacc tgtcgcgcaa ccgccgtggc gctgccgtgg cactctgagg
    10141 cgtgtgctag aattttcggt ttccaggccc ggtagctcag ttggtagagc agcggattga
    10201 aaatccgcgt gtcggcagtt cgattctgcc ccaggccacc a
//
LOCUS       DAOQTZ010000094        10213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_7366_length_10213_cov_13.741189, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000094 DAOQTZ010000000
VERSION     DAOQTZ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7366_length_10213_cov_13.741189"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PKN51_10700"
     CDS             <1..923
                     /locus_tag="PKN51_10700"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594540.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HNT85611.1"
                     /translation="ACVMITYPSTHGVFETQVKRLCEIVHQHGGRVYLDGANLNAMVG
                     LAAPGEFGGDVSHLNLHKTFCIPHGGGGPGVGPVCVVQDLVPYLPGAGTVGAVSAAPL
                     GNAAVLPISWMYIRMMGADGLRHATETAILNANYISRRLAGHYPTLYASANGHVAHEC
                     ILDLRGFKDSSGVMAEDVAKRLADYGFHAPTLSFPVANTLMVEPTESENLAELDRFID
                     AMIAIRAEIACVERGQWPQNDNPLKNAPHTAASLLQGDWSHPYPREVGAALAEQAPGS
                     VKYWPPVGRVDNVHGDRNLFCSCIPVEDLA"
     gene            920..1411
                     /locus_tag="PKN51_10705"
     CDS             920..1411
                     /locus_tag="PKN51_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT85612.1"
                     /translation="MSAAGVLVRRVDYRAAVDRSALVELLNAYAIDPMGGAEPLADEV
                     RQRLCDDLAAHPLAASFIAWRGDEPLGLANTFLGYSTFKARPLLNVHDIAVRADWRGR
                     GVGQALLAACEQHARQKRCCKLTLEVLSGNARALRSYQRFGFAPYVLDPREGQALLMQ
                     KWL"
     gene            complement(1461..2600)
                     /locus_tag="PKN51_10710"
     CDS             complement(1461..2600)
                     /locus_tag="PKN51_10710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNT85613.1"
                     /translation="MSVAQFFMPSSNLLGAGALAEAVKQMGGLGFRKALIVTDEPLVK
                     LGLASQLAGLLQAQGIASAVFDQVRPNPRVADVNAGLAALRQLDADCVVSLGGGSPHD
                     CAKGIALLATNGGRIEDYEGLDRSARPQLPLIAVNTTAGTASEMTRFTIITDETRHVK
                     MAIVDKHVTPILSVNDPQLMTGMPASLTAATGMDALTHAVEAYVSTAANPITDACALQ
                     AVALIARHLPTAVQQPQDATAREQMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYDLP
                     HGVCNALLLPHVERFNLRAAAARLDDIGRTLGAHNAELAGLDPIAAIQKLAQRVGIPP
                     NLRALGVKPEDFETLAGNAMKDVCGLTNPVQPTLHDVIGIFQAAW"
     gene            2972..3430
                     /locus_tag="PKN51_10715"
     CDS             2972..3430
                     /locus_tag="PKN51_10715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNT85614.1"
                     /translation="MPEFVDTSAPGECIFCKLVAGQIPSQRVHEDALTLAFMDLGQVN
                     PGHVLVATRRHAATLLDITAEEAAAVMRTARRVARAAQAAFAPDGITLLQASGAVAGQ
                     TVGHFHLHVLPRHTGDGVDLAWPRKEPGPAALADYAERLRAALAREEAHP"
     gene            3427..4476
                     /locus_tag="PKN51_10720"
     CDS             3427..4476
                     /locus_tag="PKN51_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNT85615.1"
                     /translation="MTAVPALPYAGTLTDVQGIEVGHFTDPRRPTGCSVVLARAGAVG
                     GVDVRGAAPGTRETDLLDPANLVGVVHAVLLAGGSAWGLDAAGGVMRWLEEQGIGLPV
                     GGAPGQIVPIVPAAVLFDLHVGDARIRPDAAAGHAACQAAGSAPPAQGNVGAGAGATV
                     GKLFGAAHAMKGGIGSAAVSVDGVTVGALIAVNALGDVVDPASGRPIAGARSADGRLH
                     GSVARALAGERPLSLLSGTQTTIGVIATDAPLTKAQCQRLAGAGHDGLARAIRPVHTM
                     SDGDTLFALATGQTRALDFNVLCSMAGEAVARACVNAVQAARSLSVAGVQLPAAIDIE
                     AAGASLERAGGQVQP"
     gene            4473..5849
                     /locus_tag="PKN51_10725"
     CDS             4473..5849
                     /locus_tag="PKN51_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNT85616.1"
                     /translation="MIRPLRQTLVLWLLSLRDLLVSAGPVLLLALGVLVAAYAWLDPQ
                     PPRQVTLATGPAGSAYAEFGTRYAQALARQGIRVRLLATEGAQANLQALRDGVADLAF
                     VRGGSADPQADSEAGIASLGALFYEPVWVFHRTDATAGRAPAATPSVAKEAPGMTRLT
                     QLRGLRVNIDQPGSGVPELVQRLLAANAIGGDDIVASQLPPEAATEALLGGSLDALVL
                     VSAPESPVVGRLLRAPRIALMDVARADAYARRFAFVQSVTLPRGVADLAADLPPHDVA
                     LLATTTSLLTREQTHPALRQLFAQAAQGLHGDTGWFNRARDFPNTRTSELPVSAEGDR
                     AINGTPPFWQRYLPFWASNLLERMWLVIGGLIVLLLPLSRVVPPLYTYRVRQRVFRWY
                     ARLRRIEARLAEAPAERELLLNELDELDRLANQTTVPLAHTDELYALRGHIEAVRRRA
                     LARPYNDA"
     gene            complement(5895..6902)
                     /locus_tag="PKN51_10730"
     CDS             complement(5895..6902)
                     /locus_tag="PKN51_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNT85617.1"
                     /translation="MTAPRRPRLVLLGTGGTIAGAGTAAEAGLSAAYRSAVVGAEALV
                     AGVPALARLADLRAEQLLQIDSADFTDALLLRLARRVAELCARDDVDGVVITHGTDTL
                     EESAYFLHLTVRSAKPIVLTGAMRPATALAADGPANLLHAVAVAAHPSAAGRGVLVVM
                     NEEIHSARDVSKVHAARLDAFASPHGPLGLVAQGAPRWYRALTRPHTTGSAFDIGALD
                     ALPLVGVVASHGNMRAEIYDAWLALGARAIVHAGFGGGTVPEYLKAPLASLRARGVLL
                     VRASRAAAGVLVRNANADDDAHGWVVTDDQNAPRARLLTALALTHTQDPLAVQQIFDC
                     Y"
     gene            complement(6942..8711)
                     /locus_tag="PKN51_10735"
     CDS             complement(6942..8711)
                     /locus_tag="PKN51_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT85618.1"
                     /translation="MSFESLNLAPAILRAVQEQGYTEPTPIQAQAIPVVLQGHDLLAG
                     AQTGTGKTAGFTLPMLHLLNGRPARRPGAIRALVLTPTRELAAQVEESVRTYGQHLSL
                     KSTVIFGGVGMNPQIERIRAGVDILVATPGRLLDLEGQDHIDLSGVEILVLDEADRML
                     DMGFIHDVKKVLALLPADKQSLLFSATFSDDIRELAAGLLRNPRSIQVTPPNTTVERI
                     RQAIYPVGRNRKKDVLAHLIGEGQWSQVLVFTRTKFGANKVADYLNERGIKAMALHGN
                     KSQSARTQALADFKSGELRALVATDIAARGIDIDELPHVVNYDIPNVPEDYVHRIGRT
                     GRAGASGEAVSLVCMDEEGFMHEIERFTRQQIPVRILDGFGPEEGERAEPIAMGRQTL
                     WGGLGKPPSREVMQAAARAARQEMMQRIRDNKAAQGQRGRAAAPGRPAPARAASDGPL
                     RADDAAQGDSDAAQGRAPQGAAATGERTGRSRRRRGRGGAGGVQGQRPGSPAGAQAPR
                     APQARATREFGFDDEDDRDLEHLPRHIDPLQTNLHGRRSAPRGPSHGAVGQPDPMRTS
                     IDLMGRGKGGGGRNKSRKGGFGR"
     gene            complement(8946..>10213)
                     /gene="aspS"
                     /locus_tag="PKN51_10740"
     CDS             complement(8946..>10213)
                     /gene="aspS"
                     /locus_tag="PKN51_10740"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNT85619.1"
                     /translation="RDYLVPSRVHDGQFFALPQSPQLYKQMLMVAGYDRYYQITKCFR
                     DEDLRADRQPEFTQIDCETSFLNEEEIRAIFQRMIREVFQQQLGVDLGAFPTMPYAEA
                     MHRFGSDKPDLRVKLEFTELTDVMTDVDFKVFSGPATTPGGRVVALRVPGGAQISRGE
                     IDGYTEFVKIYGAKGLAWIKVNEVAKGRDGLQSPIVKNIHDAAIAEILKRTGAQDGDI
                     LFFGADKTKIVNDSIGALRLKIGHSEFGKANGLFEDRWAPLWVVDFPMFEHDEEDNRW
                     VAVHHPFTSPKDGHEDLMDSDPGKCLAKAYDMVLNGWELGGGSVRIHRADVQAKVFAA
                     LNIGPEDQRAKFGYLLDALQYGAPPHGGLAFGLDRLITLMTGAESIRDVIAFPKTQRA
                     QDLLTQAPSPVDEKQLRELHIRLRNPQPA"
BASE COUNT         1406 a         3624 c         3650 g         1533 t
ORIGIN      
        1 tggcctgcgt gatgatcacc taccccagca cgcacggcgt gttcgaaacc caggtcaagc
       61 ggctgtgcga gatcgtccac cagcatggcg gccgcgtcta cctggacggt gccaacctga
      121 acgccatggt cggactggcc gcgccgggtg agttcggcgg cgacgtcagc cacctgaacc
      181 tgcacaaaac cttctgcatc ccgcacggcg gcggcggccc cggcgtcggc ccggtgtgcg
      241 tggtgcagga cctggtgccc tacctgccgg gcgccggcac ggtgggcgcc gtcagtgccg
      301 cgccgctggg caacgccgcc gtgctgccga tctcgtggat gtacatccgc atgatgggcg
      361 ccgacggcct caggcacgcc accgaaaccg ccatcctgaa cgccaactac atcagccgcc
      421 ggctggccgg ccactacccg acgctgtacg ccagcgccaa cgggcatgtg gcgcacgaat
      481 gcatcctgga cctgcgtggc ttcaaggaca gcagcggcgt gatggccgag gacgtggcca
      541 agcgcctggc cgactacggg tttcacgcgc ccacgctgtc gttcccggtc gccaacacgc
      601 tgatggtgga gccgaccgaa agcgaaaacc tggccgagct ggaccgcttc atcgacgcca
      661 tgatcgccat ccgcgccgaa atcgcctgcg tcgagcgcgg ccagtggccc cagaacgaca
      721 acccactcaa aaacgctccc cacaccgccg ccagcctgct gcagggcgac tggtcgcacc
      781 cctacccgcg cgaggtcggc gccgcgctgg ccgagcaggc accgggcagc gtcaagtact
      841 ggcccccggt gggccgcgtg gacaacgtcc atggcgaccg caacctgttt tgcagttgca
      901 ttccggtcga ggatctggct tgagcgctgc cggcgtgctg gtgcggcgcg tcgactaccg
      961 cgccgccgtg gaccgcagcg ccctggtcga gctgctcaac gcctacgcga tcgaccccat
     1021 gggcggcgct gagccgctgg ccgacgaggt gcgccagcgc ctgtgcgacg acctggcggc
     1081 gcacccgctg gcggccagct tcatcgcctg gcgcggcgac gagccgctgg gcctggccaa
     1141 caccttcctg ggctattcga ccttcaaggc ccgcccgctg ctgaacgtgc acgacatcgc
     1201 ggtgcgcgcc gactggcgcg ggcgcggcgt cggtcaggcg ctgctggcgg cgtgcgaaca
     1261 gcatgcgcgc cagaagcgct gctgcaagct cacgctggag gtgctgagcg gcaacgcacg
     1321 cgccctgcgc agctaccagc gcttcggttt tgcgccctac gtgctggatc cgcgcgaagg
     1381 tcaggcgctg ctgatgcaga aatggctgtg agccatgccg ggcgcggccg cgcctgacag
     1441 cgccggccac gcgcgggcag tcaccaggcg gcctggaaga tgccgatcac gtcgtgcagc
     1501 gtgggctgca ccgggttggt caggccgcag acgtccttca tggcgttgcc agccagagtc
     1561 tcgaagtcct ccggcttgac gcccagcgcg cgcaggttgg gcgggatgcc cacgcgctgc
     1621 gccagcttct ggatcgcggc gatcggatcg agtccggcca gttcggcgtt gtgcgcgccg
     1681 agcgtgcggc cgatgtcgtc caggcgcgcc gccgccgccc gcaggttgaa gcgctcgaca
     1741 tgcggcagca gcagggcgtt gcacacgccg tgcggcaggt cgtagaagcc gcccagctgg
     1801 tgcgccatgg catgcacgta gcccagcgag gcgttgttga acgccatgcc ggccagaaac
     1861 tgcgcgtacg ccatctgctc gcgcgccgtg gcgtcctggg gctgctgcac cgcggtgggc
     1921 aagtggcgcg cgatcagcgc caccgcctgc agcgcgcagg cgtcggtgat cgggttggcg
     1981 gcggtcgaca cataggcctc gaccgcgtgc gtcagcgcgt ccatgccggt ggcggcggtc
     2041 aggctggcgg gcatgccggt catcagctgc gggtcgttga ccgacaggat cggcgtcaca
     2101 tgcttgtcga cgatggccat cttcacgtgc cgcgtctcgt cggtgatgat ggtgaagcgt
     2161 gtcatctccg acgccgtgcc ggccgtggtg ttgacggcga tcagcggcaa ctgcggccgg
     2221 gccgaccggt ccaggccttc gtagtcctcg atgcgcccgc cgttggtggc cagcagcgca
     2281 atgcccttgg cgcagtcgtg cggcgaaccg ccgccgagcg acaccacgca gtcggcgtcc
     2341 agctggcgca gcgcggccag gccggcgttg acgtcggcca cgcgcgggtt gggcctgacc
     2401 tggtcgaaca cggcgctggc gatgccctgc gcctgcagca ggccggccag ctggctggcc
     2461 agccccagct tgaccagcgg ctcgtcggtg acgatcaggg ccttgcgaaa gccgaggccg
     2521 cccatctgct tgaccgcctc ggccagggcg cctgcgccca gcaggttgct ggatggcatg
     2581 aagaactgtg caacgctcat gtcaactcct gacgtgtgga accccatccc caccgcgtcc
     2641 gtcggtgggg gcacgctgaa cccggaatcg gcgatcgggc gcacccaccg aacgggtcaa
     2701 tgcgatcaca gccgcaagca cctgcctgca tgactcccgc cctgcagatg accagcggtc
     2761 aatgccgttt ccagcttcaa gcattgttgc ccgcagcgtg cggcgccgcc ttgagcccgg
     2821 tcaagcgaca ggtaagccgg atgcccgcat ccaccccaca ccgcgggcgc tgcgcggcgg
     2881 gttcatggct tcggattcct gaagttcagg acatccaaag gccgaatcaa taatcgtggg
     2941 gtgtccaact gccgaccctg aaagcaccgt catgcccgaa ttcgtcgaca cttccgcgcc
     3001 cggtgaatgc attttctgca aactggtggc cggtcagatt ccgtcccagc gcgtgcacga
     3061 ggatgcgctg accctggcct tcatggacct gggccaggtc aaccccgggc atgtgctggt
     3121 ggccacgcgg cgccacgccg ccacgctgct ggacatcacg gccgaggagg ccgccgccgt
     3181 catgcggacc gcgcggcgcg tggcgcgcgc ggcgcaggcg gcctttgcgc cggacggcat
     3241 cacgctgctg caggccagcg gcgcggtggc ggggcagacg gtggggcatt tccacctgca
     3301 cgtgctgccg cgccatacag gcgatggcgt cgatctggcc tggccgcgca aggaacccgg
     3361 cccggccgcg ctggccgatt acgccgaacg gctgcgcgcc gcgctggccc gcgaggaggc
     3421 acatccgtga ccgccgtgcc cgcactgccc tacgcgggca cgctgaccga cgtgcaaggc
     3481 atcgaggtcg gccacttcac cgacccgcgc cgccccaccg gctgcagcgt ggtgctggcg
     3541 cgcgccggcg ccgtgggcgg agtggacgtg cgcggtgccg cgccgggcac gcgcgaaacc
     3601 gacctgctcg accccgccaa cctggtcggc gtggtacatg ccgtgctgct cgccggcggc
     3661 agcgcctggg ggctggacgc cgccggcggc gtgatgcgct ggctggagga gcagggcatc
     3721 ggcctgccgg tgggcggcgc gccggggcag atcgtgccca tcgtgccggc ggcggtgctg
     3781 ttcgacctgc acgtgggcga cgcgcgcatc cgccccgacg ccgccgccgg tcatgcggcc
     3841 tgccaggccg ccggcagcgc gcccccggcg cagggcaacg tgggcgcggg cgccggcgcc
     3901 accgtgggaa agctgttcgg cgccgcgcac gccatgaagg gcggcatcgg cagcgccgcc
     3961 gtcagcgtgg acggcgtgac cgtgggcgcg ctgatcgccg tcaacgcgct gggcgacgtg
     4021 gtcgatccgg ccagcggccg gcccatcgcc ggcgcccgca gtgccgacgg gcgcctgcac
     4081 ggcagcgtgg cgcgggcgct ggccggcgaa cggccgctgt ccctgctgtc gggcacgcag
     4141 accaccatcg gcgtgatcgc caccgacgcg ccgctcacca aggcgcagtg ccagcgcctg
     4201 gccggcgccg gccacgacgg gctggcgcgt gccatccgcc cggtgcacac catgagcgac
     4261 ggcgacacgc tgttcgccct cgccaccggg cagacgcggg cgctggactt caacgtcctg
     4321 tgcagcatgg ccggcgaggc ggtggcacgc gcctgcgtga acgccgtgca ggccgcgcgg
     4381 agcctgagcg tggccggcgt gcaactcccg gctgctatcg atatcgaagc tgccggcgca
     4441 agcctggaaa gggctggggg ccaagttcag ccatgatccg cccgctgcgc cagaccctcg
     4501 tgctgtggct gctgtcgctg cgcgacctgc tggtgtcggc cgggccggtg ctgctgctgg
     4561 cgctgggcgt gctggtggcg gcctacgcct ggctcgaccc gcagccgcca cgccaggtga
     4621 ccctggccac cgggccggcc ggcagcgcct acgccgagtt cggcacgcgc tatgcgcagg
     4681 cgctggcgcg ccagggcatc cgcgtgcgcc tgctggccac cgaaggtgcg caggccaacc
     4741 tgcaggcgct gcgcgatggc gtggccgacc tcgccttcgt gcgtggcggc agcgccgacc
     4801 cgcaagccga cagcgaagcc ggcatcgcct cgctgggcgc cctgttctat gaaccggtgt
     4861 gggtgttcca tcggactgat gcgaccgccg gtcgtgcgcc agcagctact ccttctgtag
     4921 caaaagaggc gcccggcatg acccggctga cgcagctgcg cggcctgcgc gtgaacatcg
     4981 accagcccgg cagcggcgtg cccgaactgg tgcagcgcct gctggcggcc aacgccatag
     5041 gcggcgacga catcgtggcc agccagctgc cgcccgaggc cgccaccgaa gccctgctgg
     5101 gtggctccct ggacgcgctg gtgctggtca gcgcgcccga gtcgccggtg gtcggccgcc
     5161 tgctgcgcgc gccccgcatc gcgctgatgg acgtggcccg ggccgacgcc tatgcgcgcc
     5221 gcttcgcgtt cgtgcagtcg gtcacgctgc cgcgcggcgt ggccgatctg gcggccgacc
     5281 tgccgcccca cgatgtggcg ctgctggcca ccaccacctc gctgctgacg cgcgagcaga
     5341 cccacccggc gctgcgccag ttgttcgcgc aggcggcgca gggcctgcat ggcgacaccg
     5401 gctggttcaa ccgtgcgcgc gactttccca acacgcgcac cagcgagctg cccgtcagcg
     5461 ccgagggcga ccgcgccatc aacggcacgc cgccgttctg gcagcgctac ctgccgttct
     5521 gggccagcaa cctgctggag cgcatgtggc tggtgatcgg cggcctgatc gtgctgctgc
     5581 tgcccttgtc gcgcgtggtg ccgccgctct acacctaccg ggtgcgccag cgcgtgttcc
     5641 gctggtatgc gcggctgcgc cgcatcgagg cgcggctcgc cgaagcgccg gccgagcgcg
     5701 aactgctgct gaacgagctg gacgaacttg accgcctggc caaccagacc acggtgccac
     5761 tggcccacac cgacgagctg tacgcgctgc gcggccacat cgaggcggtg cgccgacggg
     5821 cgctggcgag accgtacaac gatgcatgaa atcggcctcc agcgcttatc catcaagcgc
     5881 cagaagctac catttcaata gcaatcgaag atctgctgaa cggccagcgg gtcctgcgtg
     5941 tgcgtgagcg ccagcgccgt cagcaggcgc gcgcgcggcg cgttctggtc gtcggtgacg
     6001 acccagccgt gggcatcgtc gtcggcgttg gcgttgcgca ccagaacccc cgccgcggcg
     6061 cgcgaggcgc gcaccagcag cacgccgcgc gcgcgcaggc tggccagcgg cgccttcaga
     6121 tactccggca cggtgccgcc gccgaagccc gcgtgcacga tggcgcgcgc gcccagcgcc
     6181 agccaggcat cgtagatttc ggcccgcatg ttgccgtggc tggccaccac gccgaccagc
     6241 ggcagcgcat cgagcgcgcc gatgtcgaac gcgctgcccg tggtgtgcgg gcgcgtcagc
     6301 gcgcggtacc agcgcggcgc gccctgcgcc accaggccca gcggaccgtg cggcgaggca
     6361 aaggcgtcca gccgcgccgc atgcaccttg ctgacgtcgc gcgcgctgtg gatttcttca
     6421 ttcatcacca ccagcacgcc gcgcccggcg gctgaggggt gcgccgccac cgccaccgcg
     6481 tgcagcaggt tggccggccc gtccgccgcc agcgcggtgg ccggccgcat ggccccggtc
     6541 agcacgatgg gcttggcgct cctgacggtc agatgcagga aataggccga ctcttccagc
     6601 gtgtcggtgc cgtgggtgat gaccacgcca tcgacgtcgt cgcgcgcgca cagctcggcg
     6661 acgcggcgcg ccagacgcag cagcagtgcg tcggtgaagt cggccgaatc gatctgcaac
     6721 agctgctcgg cacgcagatc ggccagccgc gccagtgccg gcacgccggc caccagcgcc
     6781 tcggcgccga ccacggccga acggtaggcg gccgacaacc cggcttccgc tgccgtgccg
     6841 gcgccggcaa tggtgccgcc ggtgcccagc aggacaaggc gcgggcgccg gggggcggtc
     6901 atggcaggca gggccgcggc cgtcggtctg gcatccgttg ctcagcggcc gaagccgccc
     6961 ttgcgcgatt tgttgcggcc accgccgccc ttgccgcgcc ccatcaggtc gatgctggtg
     7021 cgcatcgggt cgggctggcc gacggcgccg tggctggggc cgcgcggcgc gctgcggcgg
     7081 ccgtgcaggt tggtctgcag cgggtcgatg tggcgcggca ggtgttcgag gtcgcggtca
     7141 tcctcgtcgt cgaacccgaa ctcgcgcgtg gctcgcgcct gcggtgcgcg cggcgcctgg
     7201 gcgcccgcag ggctgccggg gcgctggccc tgcacgcccc cagcgccgcc ccgaccgcgc
     7261 cgccggcggc tgcggcccgt gcgttcgccc gtcgccgcag caccctgcgg ggcgcgcccc
     7321 tgggcggcgt cggaatcgcc ctgcgccgca tcgtcggccc gcaacgggcc atccgacgcc
     7381 gcgcgtgccg gcgccggccg gccgggcgcc gccgcccgcc cacgctggcc ctgggcggcc
     7441 ttgttgtcgc ggatgcgctg catcatctcc tgacgcgcgg cgcgcgccgc ggcctgcatc
     7501 acctcacggc tgggcggctt gcccagaccg ccccacaggg tctggcgacc catggcgatc
     7561 ggctcggccc tttcgccctc ctcggggcca aaaccgtcga ggatgcgcac cgggatctgc
     7621 tggcgcgtga aacgctcgat ctcgtgcatg aacccttcct cgtccatgca caccaggctg
     7681 accgcctcgc cactggcgcc ggcgcggccg gtgcggccga tgcggtgcac gtagtcttcg
     7741 ggcacgttgg ggatgtcgta gttcaccaca tgcggcagct cgtcgatgtc gatgccgcgc
     7801 gcggcgatgt cggtggccac cagggcacgc agctcaccgc tcttgaagtc ggccagcgcc
     7861 tgggtgcgcg cgctctggct cttgttgccg tgcagcgcca tggccttgat gccgcgctcg
     7921 ttcaggtagt cggccacctt gttggcgccg aatttggtgc gcgtgaacac cagcacctgg
     7981 ctccactggc cctcgccgat caggtgcgcc agcacgtcct tcttgcggtt gcggcccacc
     8041 gggtagatgg cctggcggat gcgctccacc gtggtgttgg gcggcgtcac ctggatgctg
     8101 cgcgggttgc gcagcaggcc ggcggccagc tcgcggatat cgtcgctgaa ggtggcggaa
     8161 aacagcaggc tctgcttgtc ggccggcagc agcgccagca ccttcttgac gtcgtggatg
     8221 aagcccatgt cgagcatgcg gtcggcttcg tccagcacca ggatttcgac gcccgacagg
     8281 tcgatgtggt cctggccctc caggtccagc aggcggccgg gggtggccac caggatgtcg
     8341 acaccggcgc ggatgcgttc gatctgcggg ttcatgccga cgccgccgaa gatcacggtc
     8401 gacttcaggc tcaggtgctg gccgtaggtg cgcacggatt cttcgacctg cgccgccagc
     8461 tcgcgcgtgg gcgtgagcac cagcgcgcgg atggcgccgg ggcgacgcgc cggccggccg
     8521 ttcagcaggt gcagcatcgg cagcgtgaag ccggccgtct taccggtgcc ggtctgggcg
     8581 ccggccagca ggtcgtgccc ctgcagcacc acgggaatgg cttgggcctg gatgggggtg
     8641 ggctcggtgt agccctgttc ttggaccgcg cgcaggatgg cgggcgccag gttcagtgat
     8701 tcaaagctca tcagattggt tgcgccggtc tctggtcggc gctggtgctg actgcccgct
     8761 ccggctgaca agccggccag tcaggggcgg cagcgggttc ttcttgaagt gggccgcccg
     8821 gcatcgtggc ggaagacccc agcggaagag ctgaccatgc ggcgaactgg tgatccgcag
     8881 gcccgcgcat tatcgcatgc cgcggtcgcc ggggcgctgt ggcccgccgg cgacgccggc
     8941 gcgcgtcagg ccggctgcgg attgcgcagc cggatgtgca gctcgcgcag ctgcttctcg
     9001 tcgaccgggc tgggcgcctg ggtcagcagg tcctgcgcgc gctgagtctt ggggaaggca
     9061 atcacgtcgc ggatcgactc ggcgccggtc atcagggtga tcaggcggtc cagaccgaag
     9121 gccaggccgc cgtgcggggg cgcgccgtac tgcagggcgt cgagcaggta gccgaacttg
     9181 gcacgctggt cctcggggcc gatgttgagc gcggcgaaca ccttggcctg cacgtcggcg
     9241 cggtggatac gcaccgagcc gccgcccagt tcccaaccgt tcagcaccat gtcataggcc
     9301 ttggcgaggc acttgccagg gtcgctgtcc atgaggtctt cgtggccgtc cttcgggctg
     9361 gtgaacgggt ggtgcacggc cacccagcgg ttgtcttcct cgtcatgctc gaacatgggg
     9421 aagtccacca cccacagcgg ggcccagcgg tcttcgaaca ggccgttggc cttgccaaac
     9481 tcgctgtggc cgatcttcag gcgcagcgcg ccgatgctgt cgttgacgat cttcgtcttg
     9541 tcggcgccga agaacaggat gtcgccgtcc tgcgcaccgg tgcgcttgag aatttcagcg
     9601 atggcggcat cgtggatgtt cttcacgatg ggcgactgca aaccgtcgcg ccccttggcc
     9661 acttcgttga ccttgatcca ggccaggccc ttggcgccgt agatcttgac gaattcggtg
     9721 tagccgtcga tctcgccgcg gctgatctgc gcgccaccgg gcacgcgcag ggcgaccacg
     9781 cgaccgcccg gggtggtggc ggggccgctg aagaccttga agtccacgtc ggtcatcacg
     9841 tcggtcagct cggtgaattc gagcttgacg cgcagatcgg gcttgtccga accgaagcgg
     9901 tgcatcgcct cggcgtaagg catggtgggg aacgcgccca gatccacgcc cagctgctgc
     9961 tggaacacct cgcggatcat gcgctggaag atggcgcgga tttcctcctc gttcaggaac
    10021 gaggtctcgc agtcgatctg cgtgaactcg ggctggcggt cggcgcgcag gtcctcgtca
    10081 cggaagcact tggtgatctg gtagtagcgg tcgtagccgg ccaccatcag catctgcttg
    10141 tagagctggg gcgactgcgg cagcgcgaag aactggccgt cgtgcacgcg gctgggcacg
    10201 aggtagtcgc gcg
//
LOCUS       DAOQTZ010000095        10019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_7540_length_10019_cov_13.519972, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000095 DAOQTZ010000000
VERSION     DAOQTZ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7540_length_10019_cov_13.519972"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..774)
                     /locus_tag="PKN51_10745"
     CDS             complement(61..774)
                     /locus_tag="PKN51_10745"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT85620.1"
                     /translation="MRPHRLDLRRIRAITLDLDDTLWPVWPTIARAEDALRAWLHTRA
                     PRTASLARDADAARQARRAAHAALPDRLHDLGALRREAIRQLLLQAGDDPALAAPAFE
                     VFLAERQRVSLFDDALPALAALSARYRVVALSNGNADVHRVGIGRHFHAAVSAAEVGV
                     GKPDPRIFRAAAQAAGVVETEVLHIGDDPHLDGHGALASGMQMAWVNRTRTPWPDDVA
                     ARPHAEVADLAALCAWLGV"
     gene            813..1859
                     /gene="hemH"
                     /locus_tag="PKN51_10750"
     CDS             813..1859
                     /gene="hemH"
                     /locus_tag="PKN51_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNT85621.1"
                     /translation="MPFRPEPEAARGQPGRTGVLLCNLGTPDQPTAAATRRYLAEFLS
                     DRRVVELPPALWQPILHGLVLRTRPAKSAAKYRSIWTPEGSPLMVWSRRQAVLLRGYL
                     GERGYDLSVRLAMRYGQPSIASQLDELRARGCTRILVLPLYPQYSGTTTASVVDAVAD
                     WARAARHVPELRFVMRFHDDPGYIRALSRVLLQHWQTHGRPDKLVMSFHGVPQRTVQR
                     GDPYHDECVTTARLLAERLGLRPDDWVLSFQSRFGKAKWVEPATQTVLEQLGRAGTSR
                     VDVICPGFVADCLETLEEIAIEGRAAFQAAGGQEFHYVPCLNDEPAWLAALAELVQDH
                     LQGWPTRAPSSSAK"
     gene            2091..2645
                     /locus_tag="PKN51_10755"
     CDS             2091..2645
                     /locus_tag="PKN51_10755"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT85622.1"
                     /translation="MPRRITGWITRGFELLIVACLTLMGILVFGNVVLRYAFNTGIAV
                     SEELSRLLFVWLIFMGAVLASAQRIHIGFDTLLHVVGPRVRRLLLLFTGLLLLAGCVI
                     FIVGGWRQTQINLANSYPVLGISYAWLYGVGLVFGVALLFPVCSNLLRAWRGEEGELT
                     EDLGAQIEAEARRVAAEADAEPRP"
     gene            2642..3919
                     /locus_tag="PKN51_10760"
     CDS             2642..3919
                     /locus_tag="PKN51_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT85623.1"
                     /translation="MTAAVFLLSLLGLIALGMPIAFALMFSGVALMLVMGQFDAQIVV
                     QNAISGADNFVLMAVPFFILAGEFMNAGGLSRRIVDMASAFVGHLRGGLGYVAIFAGI
                     LLASLSGSAVADTAALAAILVPMMRRAGYDPGRACGLMAASGIIAPVIPPSIGFLIFG
                     VAANVSITRLFLAGIVPGVMMGLVLVVAWWIVARRQQVSVTPRVPWGRRLRLLAQGGW
                     ALMLPVIIIGGLRFGIFTPTESAVVAAVYALFVGVVVYRELSARRIYQCLLVTARTTS
                     VVLLLAALAMVASYMITIADISGQVGAWLQTISDRPLVLMTAMMVVVFFAGMVLDFIP
                     IVLIFTPVFMPIVKAADIDPVYFGVVFIMNCSIGLITPPVGVVLNVVSSVGKVSMAEA
                     VRGIAPFLLALLALLALLILFPALVTVPAEWWR"
     gene            3956..4939
                     /locus_tag="PKN51_10765"
     CDS             3956..4939
                     /locus_tag="PKN51_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT85624.1"
                     /translation="MKLRISLVAGLLAAALTGPAWAAVTGKFAVTLSEKTHQGQGVAK
                     FVELVKAKSNGEIDIKPYYNAALGDDVKVTSALQGGTIEFTVPVTTTLTGMVKAYSIF
                     DFPFLFKNPEEADKVLDGPVGQKLLETLPAHGLVGLAYWENGFFNATNSKHPITRVED
                     FAGLKFRSIQAKITQETIRALGANPVPLAIPELYTALETRTIDGQGTPNAVIAALKLN
                     EVQKFLSITRHTYSPFIPLVSKAFWDKLTPAQQKILKDAAIEARTYQRQVARQQSDAA
                     QKAMAERGMKVNEVSDAERARMRERVQPVWNMFTPDVGQDLVGQVEAQLKK"
     gene            complement(4997..5872)
                     /locus_tag="PKN51_10770"
     CDS             complement(4997..5872)
                     /locus_tag="PKN51_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT85625.1"
                     /translation="MPHSSLYLRCAGREIHVLDWTPAGGAVRGTVVAWHGLARTGRDM
                     DELAAHLSARGWRVVCPDTVGRGLSQWAEQPEHEYCLAFYARLARELLAQLGIERCHW
                     VGTSMGGAIGTVCASGLFEPALAPRIQSLVLNDNAPELAQPAIERIRAYAGQPPAFAT
                     VTELEGFFRAAYQPYGFLTDAQWRRLTETSTRRLPDGRVTPHYDPAIVGQFTHHPDDY
                     LIWHHYDALTLPVLLLRGEDSDLVLRPTAEQMRLRGPGARGRLQWVEVPGCGHAPALN
                     VPEQLALVSDFIERA"
     gene            complement(5884..8016)
                     /locus_tag="PKN51_10775"
     CDS             complement(5884..8016)
                     /locus_tag="PKN51_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HNT85626.1"
                     /translation="MTKTLPRLHGLALAAIASAALLACGGSDDRFNDKPAGIGALHTQ
                     HYDGSSDDLLTAGLGASGLASALPPSYADPLQPTAAELRRAAIYNSYRALVDTTAAGG
                     YGVFYGPGVTVEGTAGGGEGKVPGSETLAFSDDGSGRENVTLMVQVPDSFDPARACIV
                     TATSSGSRGVYGAISTGEWGLKRGCAVAYTDKGTGSAAHDLRTDTVPRIDGTRTAAGS
                     AAGQAAFDAGLTAAELAAFNAATPDRLAFKHAHSGRNPEKDWGRHTLQAVRLALYVLN
                     ERYGELDASGRRRVRLHTGNTLVIASSVSNGGGAALAAAEDDREGLIDGVAVAEPAIE
                     MPANAGVAVQRGASQVPVIGRNLIDFTSYANLYQACAALAPSQAAAPFGAAFASLYNN
                     PALPIAANRCAALAQRGLLNGSSTAEQAEHALQRLREYGWEAESSVLQASHAAFEIAP
                     AVAVTFANALARARVTDHLCGFSYAATAANGSVTTLAPALLAGMFATGNGVPPSSGVN
                     LVNNLAAQGPARDFLSFTAQGVADWNLDGALCLRELVTGQGTLAARLQTGMRETQRHG
                     NLHGKPTLIVHGRDDALLPVNHTSRPYAALNRKVEGAASRLSYIEVTNAQHFDGFIGL
                     PAVLPGYDSRYVPLHLYLNRALDAVYAHLREGKALPPSQVVRTTPRGGTPGSAPALTA
                     AHVPALAASPAAGNAIGFAAGHTIVIPD"
     gene            complement(8051..9124)
                     /locus_tag="PKN51_10780"
     CDS             complement(8051..9124)
                     /locus_tag="PKN51_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT85627.1"
                     /translation="MLSRLLSHDLPRSRVLAAILIVIVLGLALAPFVFPGVKALNVAA
                     KVLIFIVLVAAFDLLLGYTGIVSFAHTMFFGIGAYGIAIACSHLGATWGALALGTLAA
                     LAVSLLLSLAIGLFSLRVRAIFFAMITLAVAAAFQTLASQLSAFTGGEDGLTFKLPEW
                     LSPSFEPFENEFFGVLIDGRIISYYLLFIVCVVLVLALLRIVNSPFGRVLQAIRENEF
                     RAEAIGYRVVVYRTLSSVLSALFATLAGCMLALWLRYNGPDTSLSFEIMMDVLLIVVI
                     GGMGTIYGAVIGSVLFLVAQSYLQDLLRLGSEAAGAVPMLSALLSPDRWLLWLGLLFV
                     LSVYYFPTGVVGRLRTWRERLVR"
     gene            complement(9130..>10019)
                     /locus_tag="PKN51_10785"
     CDS             complement(9130..>10019)
                     /locus_tag="PKN51_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT85628.1"
                     /translation="TLTVAGLAMGMIIFIIASGLTLVFGLMDVLNFGHGVFIALGAFV
                     ATSVLGAMGDWTGSSDIWRNLVAVLPAMLVAMLVAGAIGLAFERFIVRPVYGQHLKQI
                     LITMGGMIIGEELIKVIWGPQQIPLPLPTGMQGSWLIGEAAVEKYRVVAVIVGLLVFA
                     LLAWTLSRTKVGLLIRAGVQDREMVESLGYRIRRLFVGVFVAGSALAGLGGVMWGLYQ
                     QTVIPQMGAQVNILIFIVIIIGGLGSTTGALIGALLVGLMANYTGFLVPKVALFSNIA
                     LMVAVLLWRPQGVYPVANR"
BASE COUNT         1501 a         3390 c         3616 g         1512 t
ORIGIN      
        1 ggcaaggact acgtcgtcaa ggatggcgac gtgatgaact tcctgttcaa cgtctgagcg
       61 ctacaccccc agccatgcgc acagggccgc caggtcggcc acttcggcat gcggccgggc
      121 cgccacatcg tccggccacg gcgtccgggt gcggttgacc caggccatct gcatgccgct
      181 ggccagcgcg ccgtggccgt ccaggtgggg gtcgtcaccg atgtgcagca cctcggtctc
      241 gaccacgccg gcggcctggg cggcggcgcg gaagatgcgc gggtcgggtt tgccgacgcc
      301 gacctcggcg gcgctgacgg cggcgtgaaa gtggcgcccg atgccgaccc ggtgcacgtc
      361 ggcgttgccg ttgctcaggg ccaccacgcg gtagcgcgcg ctcagcgcgg ccagtgcggg
      421 cagggcgtcg tcgaacaggc tgacgcgctg gcgctcggcc agaaacacct cgaacgccgg
      481 cgccgccagc gccgggtcgt cgccggcctg cagcagcagc tggcggatcg cctcgcgccg
      541 cagcgcgccc aggtcgtgca ggcgatcggg cagggcggcg tgcgcggccc ggcgtgcctg
      601 ccgggcagcg tcggcatcgc gcgccaggct ggcggtgcgc ggcgcgcgcg tgtgcagcca
      661 ggcgcgcagg gcgtcctcgg cacgggcgat ggtgggccac accggccaca aggtgtcgtc
      721 cagatcgagc gtgatggcgc ggatgcggcg caggtcgagg cggtgagggc gcatgggcag
      781 ggacgcaggc agggcatcgg cgagaatagc ccatgccatt tcgacctgaa cccgaggccg
      841 cgcgcggcca gccgggccgc acgggcgtgc tgctgtgcaa cctgggcacc cccgaccaac
      901 ccaccgccgc cgccacacgc cgctacctgg ccgagtttct gagcgaccgg cgcgtggtcg
      961 aactgccgcc ggcgctgtgg cagccgatct tgcacggcct ggtgctgcgc acccgaccgg
     1021 caaagtcggc cgccaagtac cggagcatct ggacgcccga gggctcgccc ctgatggtgt
     1081 ggagccgcag gcaggcggtg ctgctgcgcg gctacctggg cgagcgcggg tacgacctca
     1141 gcgtgcggct ggcgatgcgc tacggccagc cgtcgatcgc ctcgcaactg gacgagctgc
     1201 gcgcgcgcgg ctgcacgcgc atcctggtgc tgccgctgta cccgcagtac tcgggcacca
     1261 ccaccgccag cgtggtcgat gccgtggccg actgggcacg cgcggcgcgc catgtgcccg
     1321 agctgcgctt cgtgatgcgc tttcacgacg acccgggcta catccgcgcg ctcagccgcg
     1381 tgctgctgca gcactggcag acccacgggc ggcccgacaa gctggtgatg agctttcacg
     1441 gcgtgccgca gcgcacggtg cagcgcggcg atccgtacca cgacgaatgc gtcaccaccg
     1501 cgcgcctgct ggccgagcgg ctggggctgc ggccggacga ctgggtgctg agctttcagt
     1561 cgcgctttgg caaggccaag tgggtggagc cggccaccca gaccgtgctg gagcaattgg
     1621 gccgcgccgg cacgtcgcgc gtggacgtga tctgcccggg attcgtggcc gattgcctgg
     1681 agacgctgga agagattgcc atcgaaggcc gcgccgcgtt ccaggccgcc ggggggcagg
     1741 agtttcatta cgtgccctgc ctgaacgacg aaccggcgtg gctggccgcg ctggccgaac
     1801 tggtccagga ccacttgcaa ggctggccga cgcgggcccc gtcgagctcg gcgaagtgat
     1861 atcaaaatga tagctgctgg cgcttgactg taaagcgccg gagagctttt tctttgttga
     1921 tttcactgcg ttcgcaggag cacccgcagg gggtgcgagg ccgctgcccg gacggggcgc
     1981 tgaccgggga aacaaccgat ggagccgccg catcagatcg ggttcaatgc aggccatctg
     2041 tccaaccgag gaacccgtca tgcaccacca ccgtcccagg accgcgcgcc atgcctaggc
     2101 gcatcaccgg ctggatcacg cgcggcttcg aactgctgat cgtcgcctgc ctgaccctga
     2161 tggggatcct ggtgttcggc aacgtcgtgc tgcgctacgc cttcaacacc ggcatcgccg
     2221 tctctgagga gttgtcgcgc ctgctgttcg tgtggctgat cttcatgggc gcggtgctgg
     2281 cctcggcgca gcgcatccac atcggcttcg acaccctgct gcacgtggtg ggcccgcgcg
     2341 tgcggcgcct gctgctgctg ttcaccgggc tgctgttgct ggccggctgc gtgatcttca
     2401 tcgtcggcgg ctggcgccag acgcagatca acctggccaa cagctacccg gtgctgggca
     2461 tctcctacgc ctggctgtac ggcgtgggcc tggtgttcgg ggtggcgctg ctgtttccgg
     2521 tttgcagcaa cctgctgcgc gcctggcgcg gtgaggaggg cgagctgacc gaggacctgg
     2581 gcgcgcagat cgaggccgag gccaggcgcg tggccgccga agccgacgcg gagccgcgcc
     2641 cgtgaccgcc gcggtgtttc tgctgtcgct gctggggctg atcgcgctcg gcatgccgat
     2701 cgcctttgcg ctgatgttct caggcgtggc gctgatgctg gtgatgggcc agttcgacgc
     2761 ccagatcgtg gtgcagaacg ccatctccgg cgccgacaat ttcgtgctga tggcggtgcc
     2821 gttcttcatc ctggccggcg agttcatgaa cgccggcggc ctgtcgcggc gcatcgtcga
     2881 catggccagc gccttcgtcg gtcacctgcg tggcggcctg ggctacgtgg ccatctttgc
     2941 cggcatcctg ctggccagcc tatccggttc ggcggtggcc gacacggccg cgctggcggc
     3001 cattctggtg cccatgatgc ggcgcgccgg ctacgacccg ggccgcgcct gcggcctgat
     3061 ggcggccagc ggcatcatcg cgccggtgat cccgccctcg atcgggttcc tgatcttcgg
     3121 cgtggcggcc aacgtgtcga tcacgcggtt gttcctggcc ggcatcgttc cgggcgtgat
     3181 gatgggcctg gtgcttgtgg tggcctggtg gatcgtggcg cgccgccagc aggtcagcgt
     3241 cacgccacgc gtgccctggg gccggcggtt gcgcctgctg gcacagggcg gctgggcgct
     3301 gatgctgccg gtgatcatca tcggcggtct gcgattcggc atcttcacgc ccaccgagtc
     3361 ggcggtggtg gcggcggtgt acgcgctgtt cgtcggtgtg gtggtgtacc gcgagctgag
     3421 cgcgcgcagg atctaccaat gcctgctggt caccgcgcgc accacttcgg tggtgctgct
     3481 gctggcggcg ctggccatgg tggcgtcgta catgatcacc atcgccgaca tctcgggtca
     3541 ggtcggcgcc tggctgcaga ccatctccga ccggccgctg gtgctgatga ccgccatgat
     3601 ggtggtggtg ttcttcgccg gcatggtgct ggacttcatt cccatcgtgt tgatcttcac
     3661 cccggtgttc atgcccatcg tcaaggcggc cgacatcgac ccggtgtatt tcggtgtcgt
     3721 gttcatcatg aactgctcga tcggcctgat cacgccgccg gtgggcgtgg tgctgaacgt
     3781 ggtcagctcg gtcggcaagg tgagcatggc cgaggcggtg cgtggcattg cgccgtttct
     3841 gctggccctg ctggcgctgc tggcgctgct gatcctgttt ccggccctgg tcaccgtgcc
     3901 agccgagtgg tggcgctgag ccgcacactg tccgatttca tccaaggaga ctcccatgaa
     3961 actgcgcatt tctctcgtcg ccggcctgct ggccgccgcc ctgaccggcc ccgcctgggc
     4021 ggcggtcacc ggcaagttcg ccgtcaccct gtcggaaaag acccaccagg gccagggcgt
     4081 ggccaagttc gtcgaactgg tcaaggccaa gagcaacggc gagatcgaca tcaagcccta
     4141 ctacaacgcc gctctgggcg acgacgtcaa ggtcacatcg gcgctgcagg gtggcacgat
     4201 cgagttcacc gtgccggtga ccaccacgct gaccggcatg gtcaaggcgt actcgatctt
     4261 cgacttcccg ttcctgttca agaaccccga ggaggccgac aaggtgctcg acggcccggt
     4321 cggccagaag ctgctggaga cgctgccggc gcacggcctg gtgggtctgg cttattggga
     4381 gaacggcttt ttcaacgcca ccaacagcaa gcacccgatc accagggtgg aagacttcgc
     4441 gggcctgaag ttccgctcca tccaggccaa gatcacccag gaaaccatcc gggcgctggg
     4501 cgccaacccg gtgccgctgg cgatccccga gctctacacc gcgctcgaga cccgcaccat
     4561 cgacggtcag ggcacgccca acgcggtgat cgccgcactc aagctcaacg aggtgcagaa
     4621 gttcctctcc atcacgcgcc atacctacag cccgttcatc ccgctggtgt ccaaggcctt
     4681 ctgggacaag ctgacgccgg cgcagcagaa aattctgaaa gacgccgcca tcgaggcgcg
     4741 cacctaccag cgccaggtgg cgcgccagca gtccgacgcc gcccagaaag ccatggccga
     4801 gcgcggcatg aaggtcaacg aggtgtcgga cgccgagcgc gcccgcatgc gcgagcgcgt
     4861 gcagcctgtg tggaacatgt tcacgcccga cgtcggacag gacctggtgg gccaggtcga
     4921 ggcgcagctc aagaagtaag ggcgcggggg ccgcgggcgc ggtctgcccg cggcatcagg
     4981 cggcgccggg cgggcctcag gcgcgctcga tgaaatcgct caccagcgcc agttgctcgg
     5041 gcacgttgag cgctggcgcg tggccgcagc cgggtacttc cacccactgc aggcgaccgc
     5101 gggcgccggg gccgcgcagc cgcatctgct cggcggtcgg gcgcagcacc aggtccgaat
     5161 cctccccgcg cagcagcagc accggcaggg tgagtgcgtc gtagtggtgc cagatcaggt
     5221 agtcgtcggg gtggtgcgtg aactggccga cgatggccgg gtcgtagtgc ggcgtcacgc
     5281 ggccgtcggg cagccggcga gtgctggttt cggtcaggcg gcgccactgg gcgtcggtga
     5341 gaaacccgta cggctggtag gcggcgcgga agaacccttc cagctcggtc acggtggcga
     5401 acgccggcgg ttggccggcg taggcccgga tgcgctcgat ggccggctgc gccagttcgg
     5461 gcgcgttgtc gttcagcacc aggctctgga tgcggggtgc cagcgcgggc tcgaacagtc
     5521 ccgaggcgca gacggtgccg atggcgccgc ccatcgaggt gccaacccag tggcagcgct
     5581 cgatgccgag ctgcgccagc agctcgcgcg ccagccgggc gtagaaggcc aggcagtact
     5641 cgtgctcggg ttgctcggcc cactggctca gcccgcgccc cacggtgtcg gggcagacca
     5701 cgcgccagcc gcgcgccgac agatgggccg ccagctcgtc catgtcgcgc ccggtgcgcg
     5761 ccagaccgtg ccaggcgacc accgtgccgc gcacggcgcc gccggcgggc gtccagtcga
     5821 gcacatggat ttcacgcccg gcgcagcgca ggtacaggga cgagtggggc atggcgttcg
     5881 acttcagtcg gggatgacga tggtgtggcc ggccgcgaag ccgatggcgt tgccggcggc
     5941 ggggctggcg gccagcgctg gcacgtgggc cgccgtcaag gccggcgcgc tgcccggcgt
     6001 gccgccacgc ggcgtggtgc gcactacctg gctgggcggc agcgccttgc cttcgcgcag
     6061 gtgggcgtac accgcgtcca gggcgcggtt caggtacagg tgcagcggca cgtagcggct
     6121 gtcgtagccg ggcagcacgg cgggcaggcc gatgaagccg tcgaaatgct gggcgttggt
     6181 gacctcgatg tagctcagcc gactggccgc accttcgacc ttgcggttca gcgccgcgta
     6241 ggggcgcgag gtgtggttga ccggcagcag ggcgtcgtcg cgcccgtgca cgatcagcgt
     6301 cggcttgccg tgcaggttgc cgtggcgctg ggtttcgcgc atgccggtct gcaggcgcgc
     6361 ggccagcgtg ccctggcccg tgaccagctc gcgcaggcac agcgcgccgt cgaggttcca
     6421 gtcggccacg ccctgggcgg tgaagctcag gaaatcgcgc gccgggccct gggccgccag
     6481 gttgttgacc aggttgacgc cgctggacgg cggcacgccg ttgccggtgg cgaacatgcc
     6541 ggccagcagt gccggcgcca gcgtggtgac gctgccgttg gcggcggtgg cggcgtagct
     6601 gaagccgcac aggtggtccg tcacgcgggc gcgcgccagg gcgttggcaa aggtgacggc
     6661 aacggcgggc gcgatctcga acgctgcgtg cgaggcctgc agcacgctgg actcggcctc
     6721 ccagccgtac tcgcgcaggc gctgcagcgc gtgttcggcc tgttcggcgg tgctgctgcc
     6781 gttcagcagc ccgcgctgcg ccagcgcggc gcagcggttg gcggcgatcg gcagcgccgg
     6841 gttgttgtag aggctggcga aggccgcgcc gaacggtgcc gctgcctgcg agggcgccag
     6901 cgcggcgcag gcctggtaga ggttggcgta gctggtgaaa tcgatcaggt tgcgaccgat
     6961 caccggcacc tggctggcgc cgcgctgcac cgccacgccg gcgttggcgg gcatttcgat
     7021 cgccggctcg gccaccgcca cgccgtcgat caggccttcg cgatcgtctt cggccgcagc
     7081 cagggcggcg ccgccgccgt tcgacacgct ggaggcgatc accagcgtgt tgccggtgtg
     7141 caggcgcacg cggcggcggc cgctggcgtc gagctcgcca tagcgctcgt tgagcacgta
     7201 cagcgccagc cgcacggcct gcagggtgtg gcggccccaa tccttctcgg ggttgcggcc
     7261 cgaatgggcg tgcttgaacg ccaggcgatc gggggtggcg gcgttgaagg cggccagctc
     7321 ggccgcggtc agcccggcgt cgaaggccgc ctggccggcg gcgctgccgg ccgcggtgcg
     7381 cgtgccgtcg atccgcggca cggtgtcggt gcgcaggtca tgggcggcgc tgccggtgcc
     7441 cttgtcggtg taggccaccg cgcagccgcg cttgaggccc cattcgccgg tggagatggc
     7501 gccgtacacg ccgcgcgagc cggaggacgt ggcggtgacg atgcaggcac gcgccgggtc
     7561 gaagctgtcg ggcacctgca ccatcagggt gacgttctcg cggcccgagc cgtcgtcgct
     7621 gaaggccagg gtctcgctgc cggggacctt gccttcgcca ccgcccgccg tgccttcgac
     7681 cgtcacgccg gggccgtaga acacgccgta gccgccggcc gccgtggtgt cgaccagggc
     7741 gcggtagctg ttgtagatgg cggcgcggcg cagctcggcc gcggtcggct gcagcgggtc
     7801 ggcgtagctg ggcggcagcg ccgacgccag cccgctggcg cccaggccgg cggtcagcag
     7861 atcgtcgctg ctgccgtcgt agtgctgcgt gtgcagcgcg ccgatgcctg ccggtttgtc
     7921 gttgaaccgg tcgtcgctgc cgccgcaggc cagcagggcc gcactggcga tggcggccag
     7981 tgccaggccg tgcaggcgcg ggagggtctt ggtcatggct tgtctcctct cggggtggtg
     8041 aaaaccgggg tcagcgcacc aaccgctcgc gccaggtgcg caggcgcccc accacgccgg
     8101 tggggaagta atagaccgac agcacgaaca gcaggcccag ccacagcagc cagcggtcgg
     8161 gcgacagcag ggccgacagc atgggcacgg cgccggccgc ctcgctgccc aggcgcagca
     8221 gatcctgcag gtagctttgc gccaccagaa acagcaccga gccgatgacg gcgccgtaga
     8281 tcgtgcccat gccgccgatc accacgatca gcagcacgtc catcatgatc tcgaacgaca
     8341 gcgaggtgtc ggggccgttg tagcgcagcc agagcgccag catgcagccg gccagcgtgg
     8401 cgaacagcgc cgacagcacc gacgacagcg tgcggtacac caccacgcgg tagccgatgg
     8461 cctcggcgcg gaattcgttt tcgcggatcg cctgcagcac ccgaccgaac ggcgagttga
     8521 cgatgcgcag cagcgccagc accagcacca cgcagacgat gaacagcagg tagtagctga
     8581 tgatccggcc gtcgatcagc acgccgaaga actcgttctc gaacggctcg aagctgggcg
     8641 acagccactc gggcagcttg aaggtcaggc cgtcttcgcc gccggtgaac gccgacagtt
     8701 gcgaggccag ggtctggaag gcggcggcca ccgccagcgt gatcatggcg aagaagatgg
     8761 cgcgcacgcg cagcgagaac agcccgatgg ccagcgacag cagcagcgac accgccagcg
     8821 ccgccagcgt gcccagcgcc agggcacccc aggtggcacc caggtgcgag caggcgatgg
     8881 cgatgccgta ggcaccgatg ccgaagaaca tggtgtgggc aaagctgacg atgccggtgt
     8941 agcccagcag cagatcgaac gccgccacca gcacgatgaa gatcagcacc ttggcggcca
     9001 cgttgagcgc cttgacgccc ggaaacacga acggtgccag cgccagaccc agcacgatga
     9061 cgatcaggat cgccgccagc acgcggctgc gcggcaggtc gtgggacagc agacggctca
     9121 gcatggcgtt caccgattgg ccaccgggta gacgccctgc ggccgccaca gcagcacggc
     9181 caccatcagg gcgatgttgg aaaacagcgc caccttgggc accagaaagc cggtgtagtt
     9241 ggccatcagg ccgaccagca gcgcgccgat cagcgctccg gtggtgctgc ccaggccgcc
     9301 gatgatgatg acgatgaaga tcaggatgtt gacctgcgcg cccatctgcg ggatcacggt
     9361 ctgctggtac agcccccaca tcacgccgcc cagcccggcc agcgcgctgc cggccacgaa
     9421 caccccgaca aagaggcgcc ggatgcggta gccgagcgac tcgaccattt ccctgtcctg
     9481 cactccggcg cgaatcagca gccccacctt ggtgcgcgac agcgtccagg ccagcagggc
     9541 gaacacgagc aggccgacga tcacggccac cacgcggtat ttctcgacgg cggcttcgcc
     9601 gatcagccac gagccctgca tgccggtggg cagcggcagc gggatctgct gcgggcccca
     9661 gatgaccttg atcagctcct cgccgatgat catgccgccc atggtgatca ggatctgctt
     9721 gagatgctgg ccatacaccg ggcgcacgat gaagcgctcg aacgccaggc cgatggcgcc
     9781 ggccaccagc atggccacca gcatggccgg cagcaccgcc accaggttgc gccagatgtc
     9841 gctcgatccg gtccagtcgc ccatggcgcc cagcacgctg gtggcgacga aggcgccgag
     9901 ggcgatgaac acgccgtggc cgaagttcag cacgtccatc agaccgaaca ccagcgtcag
     9961 gccggaggcg atgatgaaga tgatcatgcc catggccagc ccggccacgg tgagcgtca
//
LOCUS       DAOQTZ010000096         9758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_7801_length_9758_cov_13.827167, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000096 DAOQTZ010000000
VERSION     DAOQTZ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7801_length_9758_cov_13.827167"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKN51_10790"
     CDS             <1..602
                     /locus_tag="PKN51_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNT85629.1"
                     /translation="WSCVVTGTGAFFSAGLDLKDPPVNEAPLAMPNLSVTCDKPIITA
                     VEGGAIGYGAVFCLLSDMVVAGQSAYFLYPEGRMGAFQGMMGGFPGRLQYKAGLQWIM
                     TGARMPAARAREIGMVNEVVPDGTATESALALAEQINQNAPLVIQAMKALALSTVTKG
                     PMEENFRNNQLLARIVASEDYKEGVAAFLQRRPTQFKGR"
     gene            643..1614
                     /locus_tag="PKN51_10795"
     CDS             643..1614
                     /locus_tag="PKN51_10795"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNT85630.1"
                     /translation="MTLQELKARVRIPVIASPMFIVSYPELVIAQCKAGIIGSFPALN
                     ARPAHVLEEWLERITSELAEYDRQNPDRPSAPFAVNQIVHKTNDRLHHDMKVCVKYKV
                     PLVITSLGAQPEINDAVHSYGGLVMHDVINQKFAHKAIEKGADGLILVAAGAGGHAGR
                     QSPFALMQETRAWFDGPIALSGSIANGASILAAQALGADFAYIGSAFIATQEANARPE
                     YKQAILEGSAEDIVYTNFFTGVWGNYLGPSIVAAGLDPQNMPGADPSRMQFDSKTDSF
                     KAKAWKDVWGCGQGIGALHDVPPAGELVQRFIDQYHAARQRIDLRTA"
     gene            1686..2678
                     /locus_tag="PKN51_10800"
     CDS             1686..2678
                     /locus_tag="PKN51_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT85631.1"
                     /translation="MQHTHSSRRQFLGLMGAGLAGSALVLPGAASAADTLGSRPVRII
                     VPFPAGGGGDTLTRMVGDALSQQLKLPVVVENKPGGDGVIAVQELLRAAPDGHTIMFG
                     TPSALLYTPQLTPKRPPYDPLKDLAPITHFSSFTYYVYVSDALPVKTMGELVDYVRKH
                     PGKVAYGTGDSTQLVAMAQLCDQAKLDMIHVHYKGTSQAFTDFMGGRIQVMVGGFDLE
                     EQSKGKALPLATLLPERSPLKPNVPTLGEAGLAPVTTRAWTAFFAPAKTPVPIIDQLN
                     KAFAATFQNPKLQDFFKSRGSVLQASTPEAMHELLVEQMPVFAKAIKTLNLYRD"
     gene            2716..4278
                     /locus_tag="PKN51_10805"
     CDS             2716..4278
                     /locus_tag="PKN51_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT85632.1"
                     /translation="MHEPATIHHATQAACRWPDKPALICTPSGRRCSYAELDAAANQA
                     AHALGQLGLAPGDGIAFCLPNGVAFAVLMLAALRTGLYYTPLPTKASAADIAYICNDA
                     GARLLVLDAASGAHAELPALLAPECPLIISQADPTQPGAWEKLLAQQPATTPTDPRPG
                     IEMVYSSGSTGKPKGVRKALPVRNWTDADPRNAAIASAAHLGEDSVYLSTSPLYHSAP
                     HRYLSATLNAGATVVVMERFDAQASLECIDRYDCTHSLWVPTMFHRLLKLDPSVRQRY
                     RGERHRVAIHGAAPCPVPLKRAMIDWWGPILVEYYSGSEGLGSTTIDSREWLAHPGSV
                     GKPRQVAVHVLDEQFQPLPPGEVGTIFFDAPSAFSYWRDDEKTRGATSPQGWKTYGDI
                     GYLDEDGYLYLTDRRSFTVISGGVNIYPQEIENTLLAHPDVEDVAVFGVPDEDLGEKL
                     AAVIQLSAGVTADAATALALQAHCRAAGGSIKTPKLVRFCTEFPRTDSGKVQKARLRA
                     QFLQEHDAPTFG"
     gene            complement(4301..6673)
                     /locus_tag="PKN51_10810"
     CDS             complement(4301..6673)
                     /locus_tag="PKN51_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNT85633.1"
                     /translation="MPVLPDAPTAADLPLLAGLNPEQKAAVTLPPEPALILAGAGSGK
                     TRVLTTRIAWLLQTGQVSPGGVLAVTFTNKAAKEMLTRLSAMLPLNVRGMWIGTFHGL
                     ANRFLRAHHQLAGLPQAFQILDTQDQLSAIKRLVKQFNIDEERYPPRQLQWFIAGCKE
                     DGLRPRDVETRDADSRRKVEIYQLYEDQCQREGVVDFGELMLRSYELLRDHDAVRAHY
                     RRRFRHILIDEFQDTNRLQYAWVKLIAGLPAEGGGGAILAVGDDDQSIYAFRGARVGN
                     MADFVREFEVRHQIKLEQNYRSHSNILDAANELISHNTRRLGKNLRTDQGAGEPVRVY
                     EAPSDFAEAEWVVDELRQLVKQEDCPRREVAVLYRSNAQSRVLETQLFNAGVPYRVYG
                     GLRFFERAEIKHALAYLRLLENPRDDTSFLRVVNFPPRGIGARTLEQLQDAARAAGSS
                     LHDAIASVPGKAGANLGAFVARIDVLREQTAGHTLREIIEQMLDASGLIEHYRGEKEG
                     QDRIENLDELVNAAESFVTQEGFGRDAVALPMDEQGQPLTQSPASQGLDPDAPRLNEP
                     LQADADTGETQSPLAAFLTHAALEAGDNQAQAGQDAVQLMTVHASKGLEFDAVFITGL
                     EEGLFPHENALSDQGGLEEERRLMYVAITRARKRLYLSHAQTRLLHGQTRYNLRSRFL
                     DELPDGVLKWLTPQRGGFAAIAPVSIAARADWAGAAGRFGLNSSAAQPSPATSGQAIR
                     PGQTVFHNKFGEGRVLAVEGSGDDARAQVNFPRHGTKWLALAVAKLTVVE"
     gene            6804..7209
                     /locus_tag="PKN51_10815"
                     /pseudo
     CDS             6804..7209
                     /locus_tag="PKN51_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518706.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
     gene            7493..8017
                     /locus_tag="PKN51_10820"
     CDS             7493..8017
                     /locus_tag="PKN51_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85634.1"
                     /translation="MTMTQPPERFILDGETWSMNQRPHFPRAHRHIRRTPPPADLSSE
                     AGRQWTWHQSPDCPRGYVGTWTVDKGRLYLVHLSGVWQVRPGQRVFARWFSGELLVFK
                     GAPLPHVRRMHDLIHEHEVVIQVMRGRVQAMFANDDIGKKRGEVYLTPSERRHRQRQT
                     ERLRRFVDQLDEPQ"
     gene            complement(8295..8837)
                     /gene="ppa"
                     /locus_tag="PKN51_10825"
     CDS             complement(8295..8837)
                     /gene="ppa"
                     /locus_tag="PKN51_10825"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNT85635.1"
                     /translation="MPLDRISPGSKTPDEFNVIVEIPMDADPIKYEVDDETGALFVDR
                     FMTVAMYYPTNYGYIPQTLAGDGDPVDVLVVTPYPLQAGVVITCRAVGILKMEDEAGI
                     DGKVLAVPVDRISSMYSRWKKIEDVNTMRLEAITHFFQHYKDLERGKWVKVLGWEGPE
                     AAHKEVTDGLASYRKAHPQR"
     gene            9079..>9758
                     /locus_tag="PKN51_10830"
     CDS             9079..>9758
                     /locus_tag="PKN51_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNT85636.1"
                     /translation="MTGNSALILALVCGLVAVVYGFWARGWILSQDAGNARMQEIASA
                     IQAGAAAYLARQYKTIAIVGVVLAILIAVFLDLKTAIGFVVGALLSGACGFIGMNVSV
                     RANVRTAQAATKGIGPALDVAFRGGAITGMLVVGLGLLGVAGFYWFLGGTAADAHTLN
                     PLIGFAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGD
                     NVGDCAGMA"
BASE COUNT         1637 a         3294 c         3160 g         1667 t
ORIGIN      
        1 tgtggagctg cgtggtcact gggaccggcg cgtttttctc ggccggtctg gacctgaagg
       61 acccgcccgt caacgaagca ccgctggcca tgcccaacct ttcggtgacc tgcgacaagc
      121 cgatcatcac cgcggtggaa ggcggtgcga tcggctatgg cgccgtgttc tgcctgctgt
      181 cggacatggt ggtggcaggc caaagcgcct acttcctcta ccccgaaggc cgcatgggcg
      241 cgttccaggg aatgatgggg ggctttcctg gccgtctgca gtacaaggcc ggcctgcagt
      301 ggatcatgac tggggccagg atgcccgcgg cgcgcgcacg cgaaatcggc atggtcaatg
      361 aagtcgtacc tgatggcact gctaccgaaa gcgcgctggc gttggccgag cagatcaacc
      421 agaacgcgcc gctggtgatc caggcgatga aggcgctggc gctcagcacg gtcaccaagg
      481 ggcctatgga agaaaacttc cgcaacaacc agttgctcgc acgcatcgtt gccagcgaag
      541 actacaagga aggggtggcc gccttcctgc aacgacgccc gacccagttc aaggggcgct
      601 gagcgctctc atccagacac gatcattttt gaacctattc ccatgacctt gcaggaactc
      661 aaggcgcgcg tgcgcatccc ggtgattgcc tcgccgatgt tcatcgtttc gtatcccgag
      721 ctggtcatcg cccagtgcaa ggccggcatc atcggctcgt ttccggcgct caacgcccgc
      781 ccggcgcacg tgctggagga gtggctcgaa cgcatcacca gcgagctggc cgagtacgac
      841 cgacagaacc ccgatcgccc gtcggcgccc ttcgccgtga atcagatcgt gcacaagacc
      901 aacgaccgcc tgcaccacga catgaaggtg tgcgtcaagt acaaggtgcc gctggtgatc
      961 acctcgctgg gcgcgcagcc tgaaatcaac gatgccgtcc actcctacgg tgggctggtg
     1021 atgcacgatg tcatcaatca gaagttcgcg cacaaggcca ttgagaaagg cgccgacggg
     1081 ctgatcctgg tcgctgccgg cgccggcggc catgccgggc gtcagtcacc gttcgcgctg
     1141 atgcaggaaa cccgcgcctg gtttgacggg ccgatcgcgc tgtcggggtc gatcgccaac
     1201 ggcgcgtcaa tcctggcggc ccaggcgctg ggcgccgatt ttgcctacat cggctcggcc
     1261 ttcatcgcca cacaggaagc caacgcgcga cccgagtaca agcaggcgat tctggaaggc
     1321 agtgccgagg atatcgtcta caccaacttc ttcaccggcg tctggggcaa ctacctgggt
     1381 ccgtccatcg ttgccgccgg actcgacccg cagaacatgc cgggcgccga cccgtcgcgc
     1441 atgcagttcg acagcaagac cgacagtttc aaggccaagg cctggaaaga cgtctggggc
     1501 tgcggccagg gcatcggcgc tctgcacgac gtaccgcctg ccggcgagct ggtgcagcgc
     1561 ttcattgatc agtaccacgc cgcacgccag cgcatagacc tgcgcacagc ctgagcagca
     1621 ccacgacgct gatatccccg cacacgcttc gccttcaccc tcttgatcgc cacggagaca
     1681 tacacatgca acacacccat tcttcgcgcc gccagtttct gggcctgatg ggcgccggcc
     1741 tggctggcag tgcgctcgtc ctgcccggcg cggcctctgc ggccgacacg ctgggcagtc
     1801 gaccggtgcg catcatcgtg ccctttccag ctggcggcgg cggcgacacc ctgacgcgca
     1861 tggtgggcga cgcgctgtcc cagcaactca agctgccggt ggtggtggag aacaagccgg
     1921 gtggcgatgg cgtgatcgcc gtgcaggagc tgctgcgcgc cgcacccgac ggccacacga
     1981 tcatgtttgg cacgcccagt gccctgctct acacaccgca gctgacgccc aagaggcccc
     2041 cgtatgaccc gctgaaggat ctggcaccaa tcacccactt cagctccttc acctactacg
     2101 tttatgtcag cgacgccttg ccggtcaaaa ccatgggcga actggtggac tacgtgcgca
     2161 agcacccggg caaggtggcg tatggcaccg gcgattcgac ccagctggtg gccatggccc
     2221 aactgtgcga ccaagccaag ctggacatga ttcatgtgca ctacaagggc acttcgcagg
     2281 cgttcaccga cttcatgggc gggcgcatcc aggtgatggt cggcggcttt gatctggaag
     2341 aacagtccaa gggcaaggcg cttccgctgg ccacgctgct gccagagcgc agcccgctca
     2401 agcccaatgt gccaacgctc ggtgaggcgg gtctggcccc ggtgaccacc cgggcctgga
     2461 cggcattctt tgcccccgcg aaaacacccg tgccgatcat cgatcagttg aacaaggcgt
     2521 ttgcggcgac gttccagaac cccaagctgc aggacttctt caaatcgcgc ggctccgtgc
     2581 tgcaggccag cacgcccgag gccatgcatg aactcctggt cgagcagatg cccgtgttcg
     2641 ccaaggccat caaaaccctg aacctgtatc gagactgagt ctgcggcgca tgacggcgcc
     2701 tcggacgcat cctgcatgca cgaacccgcg accattcacc acgcgacgca agctgcgtgc
     2761 cgctggcccg acaagccggc gctgatctgc accccgagcg gacggcgctg cagctacgcc
     2821 gagctggacg ccgccgccaa ccaggccgct cacgcactgg gccagctggg cctggcgccc
     2881 ggtgatggca ttgccttttg cctgcccaac ggcgtggcct ttgccgtcct catgctggca
     2941 gcgctgcgca cgggtctcta ctacacaccc ctacccacca aggcgtcggc ggccgacatc
     3001 gcctacatct gcaacgatgc cggcgcacgg ttgctggtgc tggacgcagc cagcggcgcc
     3061 cacgccgagc tgcccgccct gttggcgcca gagtgccccc tgatcatcag ccaggccgac
     3121 cccacgcaac ccggcgcgtg ggagaaactg ctggcgcaac aacccgcaac cactccgaca
     3181 gacccccgtc ccggcatcga gatggtgtat tcgtcgggct cgaccggcaa acccaagggc
     3241 gtgcgcaagg cgctgcccgt gcgtaactgg accgacgcag acccccgaaa cgccgccatt
     3301 gccagcgccg cgcacctggg ggaggacagc gtctacctat caacgtcacc gctctatcac
     3361 agcgcaccgc accgctatct gtccgccacc ctgaacgccg gcgcgacggt ggtcgtgatg
     3421 gagcgctttg acgcccaagc cagcctggaa tgcatcgatc gctacgactg cacgcattcg
     3481 ctgtgggtgc ccaccatgtt ccaccggctg ctcaagctcg acccgtctgt acgtcaacgc
     3541 tatcggggag agcgccaccg ggtggccatt cacggcgcgg cgccgtgccc ggtgccgctc
     3601 aagcgcgcca tgatcgactg gtggggaccc attctggtcg agtactactc aggctccgaa
     3661 ggactgggca gcaccaccat cgactcgcgc gagtggctgg ctcatcccgg ctcagtgggc
     3721 aagccgcgcc aggtggcggt gcacgtactg gacgagcagt tccagccgct gcctccgggc
     3781 gaggtgggca ccatcttctt cgatgcgcca agcgcctttt catactggcg cgacgacgag
     3841 aagacccgtg gcgccaccag cccccagggc tggaagacct acggcgatat cggctacctg
     3901 gatgaagatg gctacctgta cctgaccgac cggcgcagct tcaccgtgat ctcgggtggc
     3961 gtgaacatct atccgcagga aatcgaaaac accctgctag cccaccccga cgtcgaggac
     4021 gtggcggtgt tcggcgtgcc tgacgaggat ctgggtgaga agctcgctgc cgtcatccag
     4081 ctgagcgcgg gcgtcaccgc cgacgccgcc accgccctgg cgctgcaagc ccattgtcgg
     4141 gctgctggcg gcagcatcaa gacacccaag ctggttcgct tttgcaccga gtttccgcgc
     4201 accgacagcg gcaaggtgca gaaggcgcgt ctgcgcgcgc agttccttca ggagcatgac
     4261 gcgccgacgt ttggctgaag cggccgcaag ccccgtcgcc ctattccacc accgtcagct
     4321 tggccaccgc cagcgccagc cacttggtgc cgtggcgcgg aaaattgacc tgggcgcgcg
     4381 cgtcgtcacc gctgccttcc acggccagca cacggccttc gccgaacttg ttgtgaaaca
     4441 ccgtctggcc ggggcggatg gcctggccgc tggtggcggg acttggctgg gccgccgacg
     4501 agttcaagcc aaatcggcct gcagcgcccg cccagtctgc gcgagctgct attgatacgg
     4561 gagcaattgc ggcaaagccg ccgcgctgcg gcgtgagcca cttgagcacg ccatctggca
     4621 gctcgtccag aaagcggctg cgcaggttgt agcgcgtctg gccgtgcagc aggcgcgtct
     4681 gcgcatggct caggtacagg cgctttctcg cgcgcgtgat ggccacgtac atcaggcggc
     4741 gctcttcctc caggccgccc tggtcgctca aggcgttctc gtgcggaaac agcccctcct
     4801 ccagaccggt gatgaacacg gcgtcgaact ccagcccctt gctggcgtgc acggtcatca
     4861 gctgcaccgc gtcctgcccg gcctgggcct ggttgtcgcc cgcctccagg gcggcgtggg
     4921 tgaggaaagc agccaacggg ctctgcgtct cgccggtgtc ggcgtcggct tgcagcggct
     4981 cgttcagcct tggcgcgtcg gggtcgagcc cctggctggc cgggctttgt gtgagcggct
     5041 ggccctgctc gtccatgggc agcgccaccg catcgcggcc aaagccctcc tgcgtgacga
     5101 agctctcggc ggcgttgacc agttcgtcca ggttctcgat gcggtcctgc ccttccttct
     5161 cgccgcggta gtgctcgatc aggccgctgg cgtccagcat ctgctcgatg atctcgcgca
     5221 gggtatggcc ggcggtctgc tcgcgcagca cgtcgatgcg cgcgacgaag gcgcccaggt
     5281 tggcgccggc cttgccgggc acgctggcga tggcgtcgtg cagcgagctg ccggcggcgc
     5341 gcgcggcgtc ctgcaactgc tccagcgtgc gcgcaccgat gccgcgtggc gggaaattga
     5401 ccacgcgcag aaagctggtg tcgtcgcgcg ggttctccag caggcgcagg taggccaggg
     5461 cgtgcttgat ctcggcgcgc tcgaagaagc gcagcccgcc atagacgcga tacggcacgc
     5521 cggcgttgaa cagctgcgtc tccagcaccc ggctctgggc gttgctgcga tacagaacag
     5581 cgacctcccg tcttgggcag tcctcctgct tgaccagctg gcgcagttca tcgaccaccc
     5641 actcggcctc ggcgaagtcg ctcggtgcct cgtagacgcg caccggctcg ccggcccctt
     5701 ggtcggtgcg caggttcttg cccaggcgcc gcgtgttgtg gctgatgagt tcgttggccg
     5761 catccagaat gttgctgtgg ctgcggtagt tctgctccag cttgatctgg tggcgcacct
     5821 cgaactcgcg cacgaagtcc gccatgttgc ccacgcgcgc gccgcgaaag gcgtagatgc
     5881 tctggtcgtc gtcgcccacc gccaggatgg cgccgccgcc gccttcggcc ggaaggccgg
     5941 cgatcagctt gacccaggcg tactgcaggc ggttggtgtc ctgaaactcg tcgatcagga
     6001 tgtggcgaaa gcggcgccgg tagtgcgcgc gcaccgcgtc gtggtcgcgc agcaattcat
     6061 agctgcgcag catcagttcg ccgaagtcca ccacgccctc gcgctggcat tggtcttcgt
     6121 agagttggta gatctcgacc ttcctgcggc tgtcggcgtc gcgcgtctcg acatcgcgcg
     6181 ggcgcaggcc gtcttccttg cagccagcga tgaaccactg cagctggcgc ggcgggtagc
     6241 gctcttcgtc gatgttgaac tgcttgacca ggcgtttgat ggccgacagc tggtcctgcg
     6301 tgtccagaat ctgaaacgcc tgcggcaaac ccgccagctg gtggtgcgcg cgcagaaagc
     6361 ggttggcaag gccgtgaaag gtgccgatcc acatgccgcg cacattgagc ggcagcatgg
     6421 ccgacaggcg cgtgagcatc tccttggccg ctttgttggt gaaggtcacg gccagcacgc
     6481 cgccgggcga gacctgcccg gtctgcaaga gccaggcgat gcgcgtggtc agcacccgcg
     6541 tcttgcccga accggcgccg gccaggatca gcgcgggctc gggcggcagg gtgacggcgg
     6601 ctttctgttc ggggttcagg ccggccagca ggggcagatc ggcggccgtg ggcgcatcag
     6661 ggagtacagg catgcgtcga ttgtaggaac cgcccccaag gcgccgccgc atcgctgcag
     6721 caacacagcg tgcatgccga gcacgtcggc cacgttcaag tggtcgcgcg gcacggcttg
     6781 ccggaagtcg cagagcgtgg tcgatggaag ttgtagtggt acatgtaaca ggtcaactcg
     6841 cgggtgagga cggcgggcgc gagctggtgg tgtgccagtg ggggctgatt ccgtggtttg
     6901 caccgcaggc gcgcctgccg cacagcaccc acaacccaca cagcgacgag ttgccgagca
     6961 aggcaaccct ggacgcggag ccagcgctgc atcgtgccgg cgcagccgtt cgacaagccg
     7021 tgctgggaga cggagcgcaa cgtgtggtgg cgccttcgcc gcgccgacgg ccaaccctgg
     7081 ggtctggcgg gactgtggaa cacctggatc gatcgcgcca gggccgaggc gcacgagagc
     7141 tacaccatgc tgacgatcaa cgccgaccag caccccctga tgcgccacat ggacaagccc
     7201 gatccgaagt tcgcgtacgg ctgagcgcct gcgctccatc gtccgatgct ggagcggcga
     7261 cgcaacactc cgcgcgcctg catggcggtg ccgatcgacc atggaacttt caagcccgat
     7321 gtcgatctaa cactaacaca gcaagcgcga gcagccatct tttcgatagc cactttgctt
     7381 ggcgcccggc ccgcccatgg cgggtttctt tttggcgggt acgcaacggc ccgcatcggt
     7441 cgccgcacgc gcggccattg catccgatcg acctcaccac cgcaatggag ccttgaccat
     7501 gacgcagcca cccgagcggt tcattcttga tggcgaaacc tggtcgatga accagcggcc
     7561 acattttccc cgcgcacacc ggcacattcg gcgcacgccc cctccagccg acctttccag
     7621 cgaagctggc cggcagtgga cgtggcacca gagcccggat tgcccgcgcg ggtacgtcgg
     7681 cacctggacg gtcgacaagg gccgcctcta cctggtgcac ctgtcaggcg tctggcaggt
     7741 acgcccgggc cagcgggtgt ttgcgcgctg gttcagcggc gagctgctgg tgttcaaggg
     7801 agcgccgctg ccgcatgtgc gcagaatgca tgacctgatt cacgaacacg aagtggtgat
     7861 ccaggtcatg cgcggtcggg tgcaggcgat gttcgccaat gacgacatcg gcaagaagcg
     7921 tggcgaggtg tacctcacac catccgagcg ccgacaccgc cagcgccaga ccgagaggtt
     7981 gcgccgcttt gtggatcagc tcgacgaacc ccagtgacgg cacctgcgcg ccgagccgcc
     8041 accccatgcc ccgcgccagc cgcagcggcg cagaacgcac ggtgttgcgc cgtccggccg
     8101 cgccccgatc gctcccgaca agggcacact cgccctgcct gcaggccccc ttgcacgggg
     8161 ttgccgattc accccacgca ggggcgctgg tgtcggcctg tccgcacgca cgacccgcaa
     8221 caagctacca aaaacatagc catccgcgct tgcctggtga gtgcggatag ctcattcgtt
     8281 accgaaatac cggctcagcg ctggggatgc gccttgcggt agctggcgag gccgtcggtg
     8341 acttccttgt gcgccgcctc ggggccttcc cagcccagca ccttgaccca cttgccgcgc
     8401 tccagatcct tgtagtgctg gaagaagtgg gtgatggctt ccagccgcat ggtgttgacg
     8461 tcctcgatct tcttccagcg gctgtacatg ctggaaatgc ggtcgaccgg caccgccagc
     8521 accttgccgt cgatgccggc ttcgtcttcc attttcagga tgccgaccgc acggcaggtg
     8581 atgaccacgc cggcctgcag cggatagggc gtgaccacca gcacgtcgac cgggtcgccg
     8641 tcgcccgcca gcgtctgcgg gatgtagccg tagttggtcg ggtagtacat cgccaccgtc
     8701 atgaagcggt cgacgaacag ggcgccggtc tcgtcgtcga cttcgtactt gatggggtcg
     8761 gcgtccatcg ggatctcgac gatgacgttg aactcatcgg gggttttgct gccgggggag
     8821 atgcgatcga ggggcatgtc ttgtgtgtgg aagggttgaa tgaaggatca aagtgcgatt
     8881 gggccggatt ttactttcgc cccccttgca ggccggcccg cccgggcaat ctgcgtaaaa
     8941 ttgcacggtt ggccaatcgc ggcacgaatc gaggacgcga ggaagcaacg cagtgagggg
     9001 gctggctgcg ttgccgctcg cgggtcagcg catccggctc ctcttgaagc gcaacgcttt
     9061 agcgaggagt gatttcaaat gacaggcaat tcggcgctga ttctggcgct cgtctgcggg
     9121 cttgtggccg tggtctacgg cttctgggca cgcggctgga tcctttccca ggacgcgggc
     9181 aacgcccgca tgcaggagat cgcctcggcc atccaggccg gtgccgccgc ctatctggcg
     9241 cggcagtaca agaccatcgc catcgtcggc gtggtgctgg cgatcctgat cgcggtgttt
     9301 cttgacctga agacggccat cggcttcgtc gtcggcgcgc tgctgtcggg cgcctgcggc
     9361 ttcatcggca tgaacgtctc ggtgcgcgcc aacgtgcgca ccgcgcaggc cgccaccaag
     9421 ggcatcggcc cggcgctgga cgtggccttc cgcggcggcg ccatcaccgg catgctggtg
     9481 gtcggcctgg gcctgctggg cgtggccggg ttctactggt tcctgggcgg cacggcagcc
     9541 gacgcacata cgctcaaccc gctgatcggc tttgccttcg gctcctcgct gatctccatc
     9601 ttcgcgcgtc tgggcggcgg catcttcacc aagggtgccg acgtgggcgc cgacctggtg
     9661 ggcaaggtcg aggccggcat ccccgaagat gacccgcgca accccgccgt gatcgccgac
     9721 aacgtgggcg acaacgtggg cgactgcgcc ggcatggc
//
LOCUS       DAOQTZ010000097         9396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_8162_length_9396_cov_12.052243, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000097 DAOQTZ010000000
VERSION     DAOQTZ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8162_length_9396_cov_12.052243"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /gene="thyA"
                     /locus_tag="PKN51_10835"
     CDS             complement(<1..446)
                     /gene="thyA"
                     /locus_tag="PKN51_10835"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNT85637.1"
                     /translation="MTAEPRAPSCDQYETFMRHVYEHGVAKADRTGTGTRSVFGHQMR
                     FDLAAGFPLVTTKKVHLKSIIYELLWFLRGDGNARWLQERGVTIWDEWANPDTGDLGP
                     VYGVQWRSWPTPDGGHIDQIAQVVDQLKTNPDSRRIIVSSWNVAELP"
     gene            complement(504..839)
                     /locus_tag="PKN51_10840"
     CDS             complement(504..839)
                     /locus_tag="PKN51_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85638.1"
                     /translation="MVPCIVVCHFQLPVPTEAQFIDIARRSAPMFRQLGERGLISKDY
                     VRGEGGAGGVYVWESRAAAEAWFTEAKLAEYTQIFGARPTLTWYDAHLTVDNKAGQVR
                     INGQPVAGS"
     gene            complement(944..1999)
                     /gene="lipA"
                     /locus_tag="PKN51_10845"
     CDS             complement(944..1999)
                     /gene="lipA"
                     /locus_tag="PKN51_10845"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNT85639.1"
                     /translation="MSDSPRPQRPVSGSKVITIHGTRAVLDGVKPSALSEVPDAGPKP
                     PWLRVRLPTGAKYQDVMEIVQSHRLSTVCAESKCPNIAECWGRGTATLMLMGSVCTRA
                     CKFCSVNTGNPKGWLDPQEPANVANAVALMDLRYVVLTSVDRDDLPDLGAGHYAACIR
                     AIHDRMPDTAVEALTPDFQGRHELVAQVLDAGLATYAQNLETVKRLTHPVRDARAGYE
                     LTLDVLKFAKRHAPGTITKTSLMVGLGETDDEIEQALDDMREAGVDVVTLGQYMRPTR
                     NHLPVQRFVTPEQFQRYRDMALAKGFLEAVSGPLVRSSYRAERVLDQDNVGLGPQAVL
                     DAIRESAAAAATPSLRC"
     gene            2375..3163
                     /gene="imuA"
                     /locus_tag="PKN51_10850"
     CDS             2375..3163
                     /gene="imuA"
                     /locus_tag="PKN51_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HNT85640.1"
                     /translation="MPTPAGVWRADALDAGAAPAFTTGHAALDAALPGGGWPLGALVE
                     LLPATDDGGVPVWPLLLPALAAHRVGQGAGTVVLVGAPASPRPAADWQPGLPALAAAG
                     LSPARLLWLPGASPVARLWAAEQALRCADVAAVLAWLPQARASDLRRLQLAAARRGDG
                     LLFVLRPAQAARTASPAPLRLRLGVDVVASTLVVHVVKRRGPPLVAPLHLPAQSAAVR
                     ALLAAAEQARAAAAARVLPFAPRPDRADAAREVDHAVDRVAVAA"
     gene            3132..>4541
                     /locus_tag="PKN51_10855"
     CDS             3132..>4541
                     /locus_tag="PKN51_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HNT85641.1"
                     /translation="MPWIALQWPPEAPPALCPAAEAAPAAPGQGQPGTPAEALGWWAL
                     QFTPRVARVDEALLLEVSGTLRLWGGLPALMHRIEAQNPAPPLIWQAPAAIGLIALAM
                     LRLRATGQALPPPAALPEALPLDVLSAARPHLPLLVRLGCRTWGQAHALPRAGLVRRC
                     GPELRAALDVAFALAPESYPWLTAPERFDQALDLHTRVDNAPALLWAAARLLTALQHW
                     LRARQLGVLALELAWTLDGKRLDGRPLPPVQSLRVRTAQPAQAMEHLRRLLAERLARV
                     SLWAPAVRLRLQALETAPWQAGNHSLLPEERRAGDPLHVFIERVSARLGPQQVLMAEP
                     LADHRPEQRQRWRPAQQASAVAVPGWADALAPTWLLPEPLPLAEQDGRPFHHGPLHLL
                     AGPHRIEAGWWPDGGDRSAQAPERDQASQPIAGPLPRRAAAPLGGSAASAAAERGGPQ
                     PAAGPLPRRAAAPLGGSAAS"
     assembly_gap    4542..4641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4842..8267
                     /locus_tag="PKN51_10860"
     CDS             4842..8267
                     /locus_tag="PKN51_10860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HNT85642.1"
                     /translation="MPEPSRKQPEAQAEAAARVRRLPRRSVAQPAPAYRPPYAELHCL
                     SHFSFQRGASSPEELVRRAWALGYTALAITDECSVAGAVRAWQALGELERSLAAADAD
                     PEFAPLHAARAAQGATEPLRLIYGAEFRFAGQGTLVALARDLAGWGALCGFITRCRGA
                     APKGQYALPLLAEALAALPGCERLWAPARAAGMAPSPLWGEGGGEGPATGVALLLELH
                     AGADDALWLAQLRALADRLGCPLVAAGDVQMHVRARKRLHDVLCAIGLGRPLHDCGLA
                     LQPNAERHLRPRARLARIYPPDTLAATLALAGRCRLRLEQLRYHYPRESVLPGLTPTE
                     TLRRHTIEGARRRLPRGVPRQIWRYVARELALIAECRYEMFFLTVHDIVREARRRGIL
                     CQGRGSAANSVVCWCLGITEADPRFSHPLLERFISRARRHEPPDIDVDFEHERREEII
                     QYIYARYGRARAALAAAVITWRTRSALRDVGAALGVDQRLIDAVASDHHGFDAQGVAT
                     RVDGLAAGLGLQPAPERISLWLELAAQLKGAPRHLSQHVGGFVLTETPLTQLVPVEPA
                     RMAGRSVIQWDKDDLEALGMLKVDVLALGMLTAIRRCLDLASRWQGAPLTRAHIVPED
                     AATYAMIRRADTVGVFQIESRAQMSMLPRLAPTTFYDLVVQVAIVRPGPITGGMVHPY
                     LKARERARRGLPIEYERSHLDAPGQPPRLARALARTLGVPIFQEQVMELSMLAAGFSA
                     EQADRLRRAMAAWKRKGGLEPFERALTDGMLARGYSQDFAHRIFEQIKGFGEYGFPES
                     HAYSFALIAYESAWLKCHQPEAWLAALLNSQPMGFYAPAQLVQDARRHGVVVLPADVS
                     ASHWDCTLEGQASRSTIEEQNMPPALIQQAPAAIKIDGSGPPRPAVRLGLRQVAGLAE
                     AAARRIVEARAGQAFASVDDLARRARLDAGDLNRLAAADALASLAGHRRQQVWQGAAW
                     RAAPALLHEAPIDEAPLALPPAPEGEAIVHDYAALGLTLRRHPLALLRARLSRWRLLT
                     AEQLARTPHGRSVRACGLVVGRQRPATAKGTIFVTLEDETGPVNVIVWPGLREDEGQR
                     NALLRARLLAVEGQWQWGGGSGGAVRHLVARRLRDLTPLLGRLAGLTTSRDFH"
     gene            8433..8996
                     /gene="thpR"
                     /locus_tag="PKN51_10865"
     CDS             8433..8996
                     /gene="thpR"
                     /locus_tag="PKN51_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNT85643.1"
                     /translation="MTTEPASPAAPQRLFTALFPPPAACAAIDAERRRWAGLPRRLRP
                     VPERMHLTLQFHDRVAPPQAQAWQAALATLRFEPFDIALVRAELWHAPHGTLAVLRAA
                     PHPALTALHERTAELAAQAGLPREPRAYKPHLTALRQAERVTLAALAAPIAWRVQAVE
                     LIWSDLLARPPRYHRLGLFAAAERPQD"
     gene            9262..>9396
                     /locus_tag="PKN51_10870"
     CDS             9262..>9396
                     /locus_tag="PKN51_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNT85644.1"
                     /translation="MSATAVGLFEAKTHLSEYVARAERGEEVIITRHNKPVAKIVPLH
                     A"
BASE COUNT         1118 a         3363 c         3512 g         1303 t
ORIGIN      
        1 ggcagctcgg ccacgttcca gctgctgacg atgatgcggc ggctgtcggg gttggtcttg
       61 agctggtcga cgacctgcgc gatctggtcg atgtggccac cgtcgggtgt gggccagctg
      121 cgccactgca cgccgtagac cgggcccagg tcgccggtgt cggggttggc ccattcgtcc
      181 cagatcgtca cgccgcgctc ttgcagccag cgggcgttgc cgtcgccgcg caggaaccac
      241 agcaattcat aaatgatgct tttcaggtgt accttcttgg tggtgaccag cggaaagccc
      301 gccgcgaggt cgaagcgcat ctggtggccg aacacgctgc gcgtgccggt gccggtgcgg
      361 tcggccttgg ccacgccgtg ctcgtacacg tggcgcatga aggtttcgta ctggtcgcag
      421 gaaggtgcgc gcggttcagc ggtcatgaca tcggctgcag gtgggtggct tcggggtgcg
      481 ggtgctgggc ggggcgccgc gtttcagctg ccggccacgg gctggccatt gatgcgcacc
      541 tggcccgcct tgttgtccac cgtcaggtgc gcgtcgtacc aggtcagcgt gggccgcgcg
      601 ccgaagatct gggtgtattc ggcaagtttg gcttcggtga accaggcttc ggcggcggcg
      661 cggctttccc acacgtagac gccgccggcg ccgccttcgc cgcgcacgta gtccttgctg
      721 atcaggccgc gttcgcccag ctggcggaac atcggcgccg agcgcctggc gatgtcgatg
      781 aattgcgctt cggtgggtac cggaagctgg aagtggcaga cgacgatgca aggcaccatg
      841 gcgtggatct cctcgggagg gcaaacgatt gtaggcaggg gcctggggcg tccctgccgg
      901 ccgggggcgg ggtggggcgc ggccctgtgt ggccgcggcg ccgtcagcag cgcagcgacg
      961 gtgtggcggc ggccgccgcc gattcgcgga tggcgtccag caccgcctgc gggcccaggc
     1021 cgacgttgtc ctggtccagc acgcgctcgg cgcggtagct ggagcgcacc agcgggccgc
     1081 tgaccgcttc cagaaagccc ttggccaggg ccatgtcgcg gtagcgctgg aactgctcgg
     1141 gcgtgacgaa gcgctgcacc ggcaggtggt tgcgggtcgg ccgcatgtac tggcccaggg
     1201 tgaccacgtc gacgccggct tcgcgcatgt cgtccagcgc ctgctcgatt tcgtcgtcgg
     1261 tttcgcccag ccccaccatc aggctggtct tggtgatggt gcccggcgcg tggcgcttgg
     1321 cgaacttcag cacatccagc gtcagctcgt agccggcgcg tgcgtcgcgc accgggtggg
     1381 tcaggcgctt gacggtttcc aggttctgcg cgtaggtggc caggccggcg tccagcacct
     1441 gcgccaccag ttcgtgccgg ccctggaagt cgggcgtcag cgcctccacc gcggtgtcgg
     1501 gcatgcggtc gtggatggcg cggatgcagg ccgcgtagtg gccggcaccc aggtcgggca
     1561 ggtcgtcgcg gtcgaccgag gtcagcacca cgtagcgcag atccatcagc gccaccgcgt
     1621 tggccacgtt ggccggttcc tgcgggtcca gccagccctt ggggttgccg gtgttgaccg
     1681 agcagaactt gcaggcgcgc gtgcacaccg agcccatcag catcagggtg gcggtgccgc
     1741 ggccccagca ttcggcgatg ttggggcact tggattcggc gcacacggtc gacagccggt
     1801 gcgattgcac gatctccatc acgtcctggt acttggcgcc ggtgggcagg cgcacgcgca
     1861 gccagggcgg cttgggcccg gcgtcgggca cttcgctcag ggcgctgggc ttgacgccgt
     1921 ccagcacggc gcgcgtgccg tggatggtga tgaccttgct gccggacacc ggacgttggg
     1981 ggcgggggga atcgctcatg gagcctcggt ctcgcgtgtg aaatgccgcg cctgccagcg
     2041 ggcgggcgca caggctcgat tctatgaggg tggggtcggc cgccgcctgc caggggattg
     2101 gcgccgccac cccccggcca gaccggcccg ccacgcccgg atcgcccccg tgctgcaaac
     2161 ccgggagcgc cgccaccatg ctccgcaagg cctgccgcga cgggcccgaa agcgcttgcc
     2221 agggcggccg ggaagggctc aaaataactg tataaataaa cagttttcga tgtctgccat
     2281 ggcccttgat tcgtccctga ccgcccgcgc gtggcgcgct gacggggtgg gcgcgcgtgc
     2341 cgtttcggcg ccgccagaca ggccgcagcc tgcggtgccg acgcccgccg gggtgtggcg
     2401 cgccgacgcc ctggacgctg gcgcagcgcc ggccttcacc acgggccacg ccgcgctgga
     2461 tgccgcgctg ccgggcgggg gctggccgct gggcgcgttg gtcgagctgc tgccggcgac
     2521 cgacgacggc ggcgtgccgg tctggcccct gctgctgccg gcgctggcgg cgcaccgggt
     2581 cgggcagggc gcgggcacgg tggtgctggt gggggcgccg gcgtcgccac ggccggccgc
     2641 cgactggcag cccggcctgc cggcactggc ggcggccggc ctgtcgccgg cgcggctgct
     2701 gtggctgccg ggcgcgtcgc ccgtcgcgcg cctgtgggcg gccgagcagg cgctgcgctg
     2761 cgccgacgtg gcggccgtgc tggcctggct gccgcaggcg cgggccagcg atctgcggcg
     2821 gctgcaactg gcggcggcca ggcgcggcga tggcctgctg ttcgtgctgc gaccggcgca
     2881 ggcggcgcgc accgcctcgc ccgcgccact gcgcctgcgg ctgggcgtgg acgtggtggc
     2941 cagcaccctg gtggtgcacg tcgtcaagcg gcgcgggccg ccgctggtcg cgccgctgca
     3001 cctgccggcg cagtccgcgg cggtgcgggc gctgctggcg gcggccgagc aggcccgggc
     3061 ggcggccgcc gcccgggtgc tgccgttcgc gccgcgcccc gatcgagccg atgccgcacg
     3121 cgaggtggac catgccgtgg atcgcgttgc agtggccgcc tgaggcgccg ccggccctct
     3181 gtcccgccgc ggaggccgcc ccggccgcgc ccggacaggg gcagcccggc acgcccgccg
     3241 aggcgctggg gtggtgggcg ctgcaattca ccccgcgcgt ggcgcgggtc gatgaagcgc
     3301 tgctgctgga agtctccggc accctgcggc tgtggggcgg gttgcccgcg ctgatgcacc
     3361 gtatcgaggc acaaaatccg gctccaccgc ttatctggca agcgccagca gctattggtt
     3421 taatagcatt ggcgatgctg cgtctgcgcg ccaccgggca ggcgctgcca ccgccggcgg
     3481 cgctgcccga ggcgctgccg ctggacgtgc tgagcgccgc ccgcccgcac ctgccgctgc
     3541 tggtgcgcct gggctgccgc acctggggcc aggcgcacgc gctgccgcgc gccgggctgg
     3601 tgcgccgctg cgggcccgag ctgcgtgccg cgctggacgt ggccttcgct ctggcgcccg
     3661 aaagctaccc ctggctgacg gcgcccgagc gcttcgacca ggcgctcgat ttgcacaccc
     3721 gggtcgacaa cgcccccgcg ctgctgtggg ccgccgcgcg cctgctcacg gcgctgcagc
     3781 actggctgcg cgcgcgccag ctcggggtgc tggcgctgga gctggcatgg acgctggacg
     3841 gcaagcgcct cgacggccgg ccgctgccgc ccgtgcagtc gctgcgggtg cgcaccgccc
     3901 agccggcgca ggccatggag cacctgcgcc gcctgctggc cgagcggctg gcgcgcgtgt
     3961 ccttgtgggc gccggccgtg cggctgcgcc tgcaggcgct ggagaccgcg ccctggcagg
     4021 ccggcaacca cagcctgctg cccgaggagc ggcgcgccgg cgatccgctg cacgtgttca
     4081 tcgagcgcgt cagcgcccgt ctgggaccac agcaggtgct gatggccgag ccgttggccg
     4141 accatcggcc cgagcagcgc cagcgctggc gaccggcgca gcaggcgtca gccgtggccg
     4201 tgcccggctg ggccgacgcg ctggcgccca cctggctgct gcccgagccg ctgccgctgg
     4261 ccgagcagga cggccggccg ttccaccacg gcccgctgca cctgctggcc gggccgcacc
     4321 gcatcgaagc cggctggtgg cccgatggcg gcgaccggtc cgctcaggca ccggagcgcg
     4381 atcaggcgtc gcaacccatc gccgggccgc tcccaaggcg ggcggcggcc cccttggggg
     4441 gcagcgcagc aagcgcagct gcggagcgtg ggggccctca gcccgccgcc gggccgctcc
     4501 caaggcgggc ggcggccccc ttggggggca gcgcagcaag cnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn ncgccgggcc gctcccaagg cgggcggcgg cccccttggg
     4681 gggcagcgca gcaagcgcag ctgcggagcg tgggggccct gtctctcgtg attactacgt
     4741 ggcgcgcaac ccggcgtccg agctgctgtg ggtgtttcgc gagcgcccga cccgcgccgg
     4801 ttcgcaggcg ggtcagacgc gctggtttct gcacggccgt tatgcctgag cccagccgca
     4861 agcagcccga ggcgcaggcc gaagcggcgg cgcgggtgcg gcgcctgccg cgccggtcgg
     4921 tggcgcagcc ggcgccggcg taccggccgc cctacgccga gctgcactgt ctgagccact
     4981 tcagcttcca gcgcggcgcc agcagccccg aagagctggt gcgccgcgcc tgggcgctgg
     5041 gctacacggc gctggcgatc accgacgaat gctcggtggc gggcgcggtg cgggcctggc
     5101 aggcgctggg cgagctggag cgctcgctcg ccgcggccga cgccgacccc gaattcgccc
     5161 cgctgcacgc cgcgcgcgcc gcgcagggcg cgaccgagcc gctgcggctg atctacggcg
     5221 ccgagttccg ctttgccggc cagggcacgc tggtggcgct ggcgcgcgac ctggcgggct
     5281 ggggcgcgct gtgcggcttc atcacgcgct gccgcggcgc ggcgcccaag gggcagtacg
     5341 cgctgccgct gctggccgag gcgctggcgg cgctgccggg ttgtgaacgg ctgtgggcgc
     5401 cggcgcgcgc ggccgggatg gctccctcgc ccctctgggg agagggtggg ggtgaggggc
     5461 cggcgacagg cgtcgccctg ctgctggagc tgcacgccgg cgccgacgac gcgctgtggc
     5521 tggcgcagct gcgcgcgctg gccgatcgtc tgggctgccc gctggtggcc gccggcgacg
     5581 tgcagatgca cgtgcgcgcg cgcaagcgcc tgcacgacgt gctgtgcgcc atcggcctgg
     5641 ggcggccgct gcacgactgc ggcctggcgc tgcagcccaa cgccgagcgc cacctgcgcc
     5701 cgcgcgcgcg gctggcgcgc atctacccgc cggacaccct ggccgctacg ctggcgctgg
     5761 ccgggcgctg ccgcttgcgg ctcgagcagc tgcgctacca ctacccgcgc gagagcgtgc
     5821 tgcccggcct gacgccgacc gaaacgctgc gccgccacac catcgagggc gcgcgccggc
     5881 gcctgccccg cggcgtgccg cggcagatct ggcgctacgt ggccagggaa ctggcgctga
     5941 tcgccgagtg ccgctacgag atgttcttcc tcaccgtgca cgacatcgtg cgcgaggcgc
     6001 ggcgtcgcgg catcctgtgc caggggcgcg gctcggccgc caactcggtg gtctgctggt
     6061 gcctgggcat caccgaggcc gacccgcgct tctcgcaccc gctgctcgag cgcttcatca
     6121 gccgggcgcg ccgccacgaa ccgcccgaca tcgacgtcga cttcgagcac gagcggcgcg
     6181 aggagatcat ccagtacatc tacgcccgct acggccgcgc gcgcgccgcg ctggccgccg
     6241 ccgtcatcac ctggcgcacg cgcagcgcgc tgcgggacgt cggcgcggcg ctgggggtgg
     6301 accagcgcct gatcgacgcc gtcgccagcg accaccacgg cttcgacgcc cagggcgtgg
     6361 ccacgcgcgt ggacggcctg gccgccggcc tggggctgca accggcgccc gagcggatca
     6421 gcctgtggct ggaactggcg gcgcagctca agggcgcgcc gcggcatctg agccagcacg
     6481 tgggcggctt cgtgctgacc gaaacgccgc tgacgcaact ggtgccggtc gagcccgccc
     6541 gcatggccgg gcgcagcgtc atccagtggg acaaggacga cctggaggcg ctgggcatgc
     6601 tcaaggtcga cgtgctggcg ctgggcatgc tgacggcgat ccgccgctgc ctcgatctgg
     6661 ccagccgctg gcagggcgcg ccgctgacgc gcgcgcacat cgtgcccgag gacgccgcca
     6721 cctacgccat gatccggcgc gccgacaccg tcggcgtgtt ccagatcgaa agccgggcgc
     6781 agatgagcat gctgccgcgc ctggcgccca ccacctttta cgacctggtg gtgcaggtgg
     6841 ccatcgtgcg cccggggccg atcaccggcg gcatggtgca cccctacctg aaggcgcgcg
     6901 agcgcgcccg ccgcggcctg ccgatcgagt acgagcgctc gcacctggac gcccccggcc
     6961 agccgccgcg cctggcgcgg gcgctggcgc gcacgctcgg cgtgccgatc ttccaggagc
     7021 aggtgatgga gctgtcgatg ctcgccgccg gcttctccgc cgagcaggcc gaccgcctgc
     7081 gccgcgccat ggcggcctgg aagcgcaagg gcgggctcga gccgttcgag cgcgcgctca
     7141 ccgacggcat gctggcgcgc ggctacagcc aggatttcgc gcaccgcatc ttcgagcaga
     7201 tcaagggctt tggcgaatac ggctttcccg aaagccatgc ctacagcttt gccctgatcg
     7261 cgtatgagag cgcctggctc aaatgccacc agcccgaggc ctggctggcg gcgctgctca
     7321 actcgcaacc catgggcttc tacgccccgg cgcagctggt gcaggacgcg cgccgccacg
     7381 gcgtcgtggt gctgccggcc gacgtgtcgg ccagccactg ggactgcacg ctggaggggc
     7441 aggcgagcag atcgaccatt gaagaacaaa atatgcctcc agcgcttatc cagcaagcgc
     7501 cggcagctat caaaattgat ggttccggcc cgccgcgccc ggccgtgcgc ctgggcctgc
     7561 ggcaggtggc gggcctggcc gaggccgccg cgcggcgcat cgttgaggcg cgggctggac
     7621 aagcttttgc cagcgtcgac gacctggcgc gccgggcgcg cctggatgcc ggcgatctga
     7681 accggctcgc cgccgccgac gccctggcga gcctcgccgg ccaccggcgc cagcaggtct
     7741 ggcagggcgc cgcctggcgc gccgccccgg cgctgctgca cgaagcgccg atcgacgagg
     7801 cgccgctggc gttgccgccg gcacccgagg gcgaggccat cgtgcacgac tacgcggccc
     7861 tgggcctgac gctgcgccgc cacccgctgg cgctgctgcg cgcgcgcctt tcgcgctggc
     7921 gcctgctgac ggccgagcag ctggcgcgca cgccccatgg ccgcagcgtg cgcgcctgcg
     7981 gcctggtggt cgggcgccag cggccggcca ccgccaaggg caccatcttc gtcaccctgg
     8041 aagatgaaac cgggccggtc aacgtcatcg tctggcccgg cctgcgcgaa gacgagggcc
     8101 agcgcaacgc cctgctgcgc gcgcgcctgc tggcggtcga aggccagtgg cagtggggcg
     8161 gcggcagcgg cggcgcggtg cgccacctgg tggcgcgccg cctgcgcgac ctcacgccgc
     8221 tgctgggccg gctggccggg ctgaccacga gccgggactt tcattgaacc tggtgtggcg
     8281 ttgcgcgcca tctggaacat gaaaccgcgt cttctcgccc atgatgcctt tgcaaagccg
     8341 cagcgtgccc gtatcgggcg gacagcgccg atccgatttc agtgtttcag tggactgggt
     8401 tgaactgggc gtcggcgcgt cacaatgccg gtgtgaccac cgaacctgcc tcgcccgctg
     8461 cgccgcagcg cctgttcacg gcgctgtttc cgccgcctgc ggcctgtgcg gccatcgacg
     8521 ccgagcgccg gcgctgggct ggcctgccgc gccgcctgcg gccggtgccc gagcgcatgc
     8581 acctgacgct gcagtttcac gaccgggtgg caccaccgca ggcgcaggcc tggcaggcgg
     8641 ccctggccac gctgcgcttc gagccgttcg acatcgccct ggtgcgcgcc gaactgtggc
     8701 acgccccgca tggcaccctg gcggtgctgc gcgcggcgcc ccacccggcc ctgacggcgc
     8761 tgcatgaacg caccgccgaa ctggcggcgc aggccggcct gccacgcgag ccgcgcgcgt
     8821 acaaaccgca cctgacggcg ctgcgccagg ccgagcgcgt gacgctggcg gcgctggcgg
     8881 cgcccatcgc ctggcgcgtg caggcggtcg agctgatctg gtccgacctg ctggcgcggc
     8941 cgccgcgcta ccaccggctg ggcctgtttg ccgccgccga aaggccgcag gactgacggg
     9001 cggcaccgcc cgcatcggcc catgatctgc cctgccagag gatcggctgc cgacaaaggc
     9061 atcggcttgc agcgcacatg gggcaagcgc gggcagctat ggtttctgat ggatcgggcg
     9121 cgggccgagg cacggtgtgc agcgcgctgc gcggcccatg gaacgcccga ggcgggtgcg
     9181 cctgcgcggg ctgagtctcg gcgcgggcaa tggggctaaa atctggttga ttcaaccagt
     9241 ttgaatgtca gggagtccgc catgtccgcc accgccgtgg gcctgttcga ggccaagacc
     9301 cacctcagcg agtacgtcgc ccgcgccgag cgaggcgagg aggtgatcat cacccgccac
     9361 aacaagccgg tggccaagat cgtgccgctg catgcc
//
LOCUS       DAOQTZ010000098         9356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_8207_length_9356_cov_13.231588, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000098 DAOQTZ010000000
VERSION     DAOQTZ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8207_length_9356_cov_13.231588"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..77)
                     /locus_tag="PKN51_10875"
     tRNA            complement(2..77)
                     /locus_tag="PKN51_10875"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Ala,seq:cgc)
     gene            complement(104..1450)
                     /locus_tag="PKN51_10880"
     CDS             complement(104..1450)
                     /locus_tag="PKN51_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT85645.1"
                     /translation="MSLYAQLQQRAAAGRPIRVGLIGAGKFGSMYLAQVPRTPGVHLA
                     AIADLSPAAARANLARVGWRPEQAQAASIDEALQRGSTHVGEDWQALVRHPAIDVVVE
                     CTGHPIAAVDHCLAAFDAGKHVVNVTVEADAFCGPLLARRAQEAGVVYSMAFGDQPAL
                     ICDLVDWARTCGFPVVAAGRGHKWLPHFCESTPETVWGYYGLSPEQAARGGLNPKMFN
                     SFLDGSKPAIESTAVANATGLAVPTGGLQYPSGSIADIPRLTRPRSAGGVLEKKGMVE
                     VISSLRPDGTPIDYDIRMGVWVTVEAETDYIRHCFEEYNAHTDDSGRYFTLYKRWHLI
                     GLEVGLSVASVALRREATGVAQGWHADVVATAKRDLKPGDVLDGEGGTTVWGKLQPAS
                     RSVAMGGLPLGLAHDVKVLRPVTKGQCLSWDDVAMDTRTRAWQLRQQMQQLLTPAD"
     gene            complement(1529..2398)
                     /locus_tag="PKN51_10885"
     CDS             complement(1529..2398)
                     /locus_tag="PKN51_10885"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT85646.1"
                     /translation="MTLTLLGTGRMGLPMARRLCQAGHELHVWNRSIDKAQPLAADGA
                     TVHGDLASAAAAGDIVISLLENGPVVASVLFEQGAAAALRPGSLFIDMASIKPAEARE
                     HAARLAERGVRHLDAPVSGGTVGAEAGTLAIMVGGEVQDFERARPVFDVLGRATHVGP
                     HGAGQLAKLANQMIVGITIGAVAEALLLCQKGGADPAKVRQAIGGGFAQSRILELHGE
                     RMVTRDFAPRARMTVQLKDLRNALDTAQAMGFEAPVTAALERLYADGIAHGDSELDHA
                     GLFVELARRNGMS"
     gene            2531..3406
                     /locus_tag="PKN51_10890"
     CDS             2531..3406
                     /locus_tag="PKN51_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT85647.1"
                     /translation="MARPNDSSRRDQPDTQWLERMYNNLRRVPDHGDYFARWAAESAL
                     VRRSLPCQLDLPYGDAAGETLDAFPGERPRSPIVVFIHGGYWKAMDKSQHSFVVPALR
                     ALGAAVVVPNYTLAPKASIPDITLQMVRAVAWCGRHASALGGDARRLVVIGHSAGGQL
                     AAMLLGCAWSAFDPALPPRLVRAALGISGLYDLQPLLHTPSLQEVLRLTPRQVQVASP
                     ARLPAPAHGRLISVVGGEESGEYLRLNKLIQQAWGRERVPVAAELPGLNHFSILDTLA
                     APRSRLHRLAAGLLV"
     gene            complement(3477..4700)
                     /locus_tag="PKN51_10895"
     CDS             complement(3477..4700)
                     /locus_tag="PKN51_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HNT85648.1"
                     /translation="MSSAVAQAPHALLTHLQGGAEAFVALRRDIHQHPELGYQEVRTS
                     DLVAERLTAWGYQVTRGLGGTGLVGQLRRGGGQKRLGLRADMDALPIQEATGLPWASQ
                     HAGRMHACGHDGHTAMLLAAAHHIAEHGRFSGTLNLIFQPAEEGLGGAKAMMDDGLFE
                     RFPCDAIFAMHNMPGYPQGKLLLREGPLMASSDGLTITLTGKGSHAAVPQHGIDPVVA
                     GSSIVLGLQSIVARNVDPQAMAVITVGAFQAGHAHNVIPGEAVLKLSVRALDRKVRAT
                     LLRRIEELVDAQARAFGVGWRMERVTGYPVLVNTRAETEFARRVATELVGAARVEPQT
                     VALSGSEDFAFMLEQVPGSYLLIGNGDGSDDGANGGHGACMVHNPGYDFNDANLPVGA
                     AYWALLVERFLSDAV"
     gene            complement(4697..5695)
                     /locus_tag="PKN51_10900"
     CDS             complement(4697..5695)
                     /locus_tag="PKN51_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT85649.1"
                     /translation="MTSARRPLLAAIVCAVALGGFAGTAGANSFPSKPVSIMVPYPAG
                     GISDTIARRVNVPLGQALGQTVIVENLGGASGSIAAQKVLNAPADGYNMFQGSPNELI
                     LAPLAIPGIKYKPEDFRQVHRISLGPMAIFARANLPAGNADELSAYAAKVAKEGKPMT
                     YASVGVGSFYHLLGGKMSKDIGVEMTHVPYKGAADVARDLAGGQVDIFITPYGPSLVE
                     LVKQGKLKVVAALSTQRQPAIPDVPTTAESKALKNFQYQIGTGYFVKRSTPEPVVQTL
                     HKGLQKVLSDAEVKAALEANGAQVSPPQTLAEADAAFKQETATYQAIARSIGLVQP"
     gene            5816..6715
                     /locus_tag="PKN51_10905"
     CDS             5816..6715
                     /locus_tag="PKN51_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT85650.1"
                     /translation="MNLRQLDHLVALADTGSFTRAAARLHLTQSALSRSVQALEDELG
                     APLVDRIGKRSELTPLGQAVLARARRIGLEAAEIRRSAELLRQGEAGTMRVGLGSGPG
                     GLLMTALLTHVAGLHPKVRVQIARGSTELQLQQLRARELDALVLDARRVAPAPDLVIE
                     PAVELRAGFVCRAGHPLAASAAVTFEMLMRYPVATTPLSDEVARVLVARYGPGAEPAR
                     MASLVCEDVGSLLDAVAQTDAVFLGAVAAARAAIDADRLVELRMTPPLDSCARYAYVR
                     LAGRTEAPVMQVLRRFAAERLRD"
     gene            complement(6952..7929)
                     /locus_tag="PKN51_10910"
     CDS             complement(6952..7929)
                     /locus_tag="PKN51_10910"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HNT85651.1"
                     /translation="MEIFDYDHILLLPRKCRVQSRSECDAGIDFGGRRFRLPVVPANM
                     KTVVDERLCIWLAEHGYFYVMHRFDFDNLAFVRDMKARGLYASISVGIKPADYACIDQ
                     LAAEGLTPEYITIDIAHGHSDQVRDMITHIKARLPASFVIAGNVATPEAVIDLENWGA
                     DATKVGVGPGKVCITRLKTGFGTGGWQLSAVKWCARVATKPIVADGGIRHHGDIAKSI
                     RFGATMIMIGSLFAGHEESPGRTVEVDGVQYKEYYGSASDFNKGEYKHVEGKRILEPI
                     KGRLADTLTEMEQDVQSSISYAGGRKLMDVRKVNYVILGGDNAGEHLLM"
     gene            complement(8065..8889)
                     /locus_tag="PKN51_10915"
     CDS             complement(8065..8889)
                     /locus_tag="PKN51_10915"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNT85652.1"
                     /translation="MDVLLMVKAALMGVVEGLTEFLPISSTGHLILAGSLLGLTGDKV
                     KVFEIAIQTGAILAVVIVYWHKIRATLVELPRSRQAQRFALNVVIAFVPAVLLGLLFG
                     KAIKAHLFTPQVVASTFILGAFVILWAERRPQSAARVHRVDDMTALDALKVGLVQCFA
                     MIPGTSRSGATIIGGMLLGLSRQAATDFSFFLAIPTLIGAGAYSLWKERALLSWADLP
                     LFGIGLLFSFLSAWICVRWLLRYIASHSFVPFAWYRLAFGAVVLLTWWMGWVAWVD"
     gene            9127..>9356
                     /locus_tag="PKN51_10920"
     CDS             9127..>9356
                     /locus_tag="PKN51_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HNT85653.1"
                     /translation="MNVPHTSYTPEQIDRLARRRAGAKMGWFIHAFVYLCVNAGLALL
                     ALSHGRDWYAYPLLGWGLGLALHGAAVWLMGPG"
BASE COUNT         1409 a         3241 c         3229 g         1477 t
ORIGIN      
        1 gtggtggaga ggaggaggat cgaactcccg acctccgcat tgcgaacgcg gcgctctccc
       61 agctgagcta cccccccacg aaggacggcc attttagcgc aattcagtcg gccggcgtga
      121 gcagctgctg catctgctgg cgcagttgcc aggcgcgcgt gcgcgtgtcc atggcgacgt
      181 cgtcccagct caggcactgg cccttggtga ccgggcgcag caccttcacg tcgtgcgcca
      241 ggcccagcgg caggccgccc atggccaccg accttgaagc gggctgcagc ttgccccata
      301 cggtggtgcc gccttcgcca tcgagcacgt cgccgggctt gaggtcgcgc ttggcggtgg
      361 ccaccacgtc ggcgtgccag ccctgcgcca cgccggtggc ttcgcggcgc agcgccacgc
      421 tggccacgct caggccgacc tccaggccga tcaggtgcca gcgcttgtac agggtgaagt
      481 agcggccgct gtcgtcggtg tgggcgttgt attcctcgaa gcagtggcgg atgtagtcgg
      541 tctcggcctc caccgtcacc cacacgccca tgcggatgtc gtagtcgatc ggcgtgccgt
      601 cggggcgcag gctggagatg acctcgacca tgcctttctt ttccagcacg ccgcctgcgc
      661 tgcgcgggcg cgtcagcctg gggatgtcgg caatgctgcc gctcggatac tgaagtccgc
      721 ccgtcggcac cgccagcccg gtggcgttgg cgaccgccgt gctttcgatg gccggcttgc
      781 tgccgtccag aaagctgttg aacatcttgg ggttcaggcc gccgcgcgcg gcctgctcgg
      841 ggctcaatcc gtagtagccc cacacggttt cgggcgtgct ctcgcagaag tgcggcagcc
      901 atttgtggcc gcgcccggcg gcgaccaccg gaaagccaca ggtgcgcgcc cagtccacca
      961 ggtcgcagat cagcgccggc tggtcgccaa aggccatcga atacacgacg cccgcttcct
     1021 gcgcgcggcg cgccagcagc gggccgcaga aggcgtcggc ctcgacggtg acgttgacca
     1081 cgtgcttgcc cgcgtcgaag gcggccaggc agtggtcgac cgcggcgatc gggtggccgg
     1141 tgcattcgac caccacgtcg atcgccgggt ggcgcaccag ggcctgccag tcctcgccca
     1201 cgtgggtgct gccgcgctgc agcgcctcat cgatggacgc ggcctgcgcc tgctcgggcc
     1261 gccagcccac gcgcgccagg ttggcgcggg cggcggcggg cgacagatcg gcgatggccg
     1321 ccaggtgcac gcccggcgtg cgcggcacct gcgccaggta catggagccg aatttgccgg
     1381 cgccgatcag gccgacgcgg atcgggcggc cggcggcggc gcgctgctgc agttgagcgt
     1441 acagactcat ggtcaatccg tgatgcaaag ccgtggttgt aacacccggc ccgccctgcc
     1501 atgcgcgccg ccgcggccgg cgcgacgttc agctcatgcc gttgcgccgc gccagctcga
     1561 cgaacaggcc ggcatggtcc agctcgctgt cgccgtgggc aatgccgtcg gcgtacaggc
     1621 gctccagcgc ggcggtgacg ggcgcttcga agcccatggc ctgggcggtg tccagcgcat
     1681 tgcgcaggtc cttgagctgc accgtcatgc gcgcgcgcgg cgcgaagtcg cgcgtgacca
     1741 tgcgctcgcc atgcagctcc agaatgcggc tttgcgcaaa gccaccgccg atggcctggc
     1801 gcaccttggc cgggtcggcg ccgcccttct ggcacagcag cagcgcctcg gccacggcac
     1861 cgatggtgat gccgacgatc atctggttgg ccagcttggc cagctgcccg gcgccatgcg
     1921 ggccgacgtg ggtggcgcgg cccagcacat cgaacaccgg ccgagcacgt tcaaagtcct
     1981 gcacctcgcc gccgaccatg atggccagcg tgccggcctc ggcgcccacg gtgccgccgg
     2041 acaccggcgc gtccaggtgg cgcacgccgc gctcggccag ccgcgcggcg tgctcgcgcg
     2101 cttcggccgg cttgatggaa gccatgtcga tgaacaggct gcccggccgc agggccgccg
     2161 ccgcgccctg ctcgaacagc acgctggcca ccaccgggcc gttctccagc aggctgatga
     2221 cgatgtcgcc ggccgccgcg gcggaggcca gatccccatg cacggtggcg ccgtcggccg
     2281 ccagcggctg cgccttgtcg atgctgcggt tccacacgtg cagctcatgg ccggcctgac
     2341 acagccggcg tgccatcggc aggcccatgc gaccggtacc cagcagcgtc agcgtcatct
     2401 cgaatgctcc ttgttttgta gctgcctgcg ctcaccccgc aggcgtcgaa gccgattgta
     2461 ggcagcaaga cacagtctgg cgcacggcag cgcgcggcgg ccggccggtt tgcgacaata
     2521 gcgctccacc atggcccgcc ccaacgattc cagccgccgc gaccaacccg acacccagtg
     2581 gctggagcgc atgtacaaca acctccggcg ggtgcctgac cacggcgatt actttgcccg
     2641 ctgggcggcc gagtcggcgc tggtgcggcg cagcctgccc tgccagctcg atctgcccta
     2701 tggcgacgcc gcaggcgaga cgctggacgc cttcccgggc gagcgcccgc gctcgcccat
     2761 cgtggtgttc atccacggcg gctactggaa ggcgatggac aagtcgcagc acagctttgt
     2821 ggtgccggcg ctgcgcgcgc tgggggcggc ggtggtggtg cccaattaca cgctggcgcc
     2881 caaggccagc atccccgaca tcacgctgca gatggtgcgc gccgtggcct ggtgcgggcg
     2941 ccacgcgagc gcgctgggcg gtgacgcgcg ccgcctcgtg gtgatcggcc attccgccgg
     3001 cggccaactg gcggccatgc tgctgggctg cgcctggagc gccttcgacc ccgccctgcc
     3061 gccgcgcctg gtgcgtgcgg cgctgggcat ctcgggcctg tacgacctgc agccgctgct
     3121 gcacacgccc agcctgcagg aggtgctgcg cctgacgccg cgccaggtcc aggtggccag
     3181 cccggcgcgc ctgccggcgc ccgcgcacgg gcggctgatc agcgtggtgg gcggcgagga
     3241 aagcggcgaa tacctgcgcc tgaacaagct gatccagcag gcctggggcc gcgaacgggt
     3301 gccggtggcg gccgagctgc cggggttgaa ccacttcagc atcctcgaca ccctggctgc
     3361 gccgcgcagc cggctgcacc gtctggcggc cggcctgctg gtctgaatgc tatatcattg
     3421 atagctgtcg gcgcacgacc ggcgcgcgcc agaggtcaat ttgacgctca atgccgtcag
     3481 accgcgtcgc tcaggaagcg ctcaacgagc agcgcccaat aggccgcacc gaccggcagg
     3541 ttagcgtcgt tgaagtcgta gccggggttg tgcaccatgc aggcgccatg gccaccgttg
     3601 gcgccgtcgt cgctgccgtc gccattgccg atcagcaggt agctgccggg cacctgttcg
     3661 agcatgaagg cgaagtcctc gctgcccgac agcgccacgg tctgcggctc gacgcgcgcc
     3721 gcgccgacca gctcggtggc cacccggcgg gcgaactcgg tctcggcccg ggtattgacc
     3781 agcaccgggt agccggtgac cctctccatg cgccaaccga cgccaaaggc cctggcctgc
     3841 gcgtccacca gttcctcgat gcgccgcagc agggtggcgc gcaccttgcg gtcgagcgcg
     3901 cgcacgctga gcttgagcac cgcctcgccc ggaatcacgt tgtgcgcgtg gccggcctgg
     3961 aaggcaccga cggtgatcac cgccatggcc tgtggatcga cgttgcgcgc cacgatggat
     4021 tgcaggccca gcacgatgct gctgcccgcc accaccgggt cgatgccgtg ctgcggcacg
     4081 gcggcgtggc tgcccttgcc ggtgagggtg atggtcagac cgtcggacga ggccatcagc
     4141 gggccttcgc gcagcagcag cttgccctgc ggatagcccg gcatgttgtg catggcgaag
     4201 atggcgtcgc agggaaagcg ctcgaacagg ccgtcgtcca tcatcgcctt ggcgccgccc
     4261 aggccttctt cggccggctg gaagatcagg ttcagcgtgc ccgaaaagcg cccgtgctcg
     4321 gcaatgtggt gcgccgccgc cagcagcatg gcggtgtggc cgtcgtggcc gcaggcgtgc
     4381 atgcggccgg cgtgctggct ggcccagggc agtccggtgg cctcctggat cggcagcgcg
     4441 tccatgtcgg cgcgcaggcc caggcgcttt tggccgccgc cgcgccgcag ttgcccgacc
     4501 aggccggtgc cacccaggcc gcgcgtgacc tggtagcccc aggcggtcag gcgctcggcc
     4561 accaggtcgc tggtgcgcac ctcctggtag cccagctcgg ggtgctggtg gatgtcgcgg
     4621 cgcagcgcga cgaaggcctc ggcaccaccc tgcaggtgcg tcagcagcgc gtgcggcgct
     4681 tgagcgacgg cgctgctcat ggctgcacca gcccgatgct cctggcgatc gcctggtagg
     4741 tggcggtctc ctgcttgaag gcggcgtcgg cctcggccag cgtctgcggc ggcgacacct
     4801 gggcaccgtt ggcttccagg gcggccttga cttcggcatc gctcagcacc ttctgcagac
     4861 ccttgtgcag cgtctgcacc accggctccg gcgtggagcg cttgacgaaa tagccggtgc
     4921 cgatctggta ctggaagttc ttcagcgcct tgctttccgc cgtggtcggc acatcaggaa
     4981 tggccggctg gcgctgcgtc gacaaagcgg ccacgacctt gagcttgccc tgcttgacca
     5041 gctccaccag cgaagggccg tagggcgtga tgaagatgtc cacctgaccg cccgccagat
     5101 cgcgcgccac gtcggccgcg cccttgtagg gcacgtgggt catctcgacg ccgatgtcct
     5161 tgctcatctt gccgcccagc aggtggtaga aggagccgac gccgacgctg gcgtaggtca
     5221 tgggtttgcc ctccttggcc accttggccg cgtacgccga cagttcatcg gcattgccgg
     5281 ccggcagatt cgcgcgggca aagatggcca tcgggcccag cgagatgcgg tgcacctggc
     5341 ggaagtcttc cggcttgtat ttgatgccgg ggatggccag cggcgccagg atcagctcat
     5401 tgggcgagcc ctggaacatg ttgtagccgt cggccggcgc gttcagcacc ttctgcgcgg
     5461 cgatggagcc gctggcgccg cccaggttct cgacgatcac cgtctggccc agcgcctgac
     5521 ccagcggcac gttgacgcgc cgggcgatgg tgtcggagat gccgcctgcg gggtacggca
     5581 ccatgatgga caccggcttg gacggaaagc tgttggcacc agcggtgccg gcaaagccgc
     5641 ccagcgcgac agcgcagacg atggcggcaa gcagtgggcg gcgggcagag gtcatgaaaa
     5701 gtgtctcctt gggatttgct cagtctggcg atttcggccg ggcgcaagta aatcgcaatc
     5761 acgagaatgc gtcaaacgaa ttgtgttcaa acaaatgatt ccctaaattc atgatatgaa
     5821 cctgcgtcag ctcgatcacc tggtggcgct ggccgacacc ggttcgttca cccgcgccgc
     5881 cgcgcgcctg cacctgacgc agtcggcgct cagccgcagc gtgcaggcgc tggaggacga
     5941 actgggcgcg ccgctggtcg accgcatcgg caagcgcagc gagctcaccc cgctgggcca
     6001 ggcggtgctg gcacgcgcac gccgcatcgg gctggaggcg gccgaaatcc gccgcagcgc
     6061 cgagctgctg cgccagggcg aggccggcac catgcgggtg gggctgggct cggggccggg
     6121 cgggctgctg atgaccgcgc tgctcaccca tgtggcggga ttgcacccca aggtgcgggt
     6181 acagatcgcg cgcggctcca ccgagctgca actgcagcag ctgcgcgcac gcgagctgga
     6241 cgcgctggtg ctcgacgcgc gccgcgtggc gccggcgccc gatctggtga tcgagccggc
     6301 ggtcgagctg cgcgccggct tcgtctgccg cgccggccat ccgctggccg ccagcgcggc
     6361 ggtgaccttc gagatgctga tgcgctaccc ggtggccacc accccactgt cggacgaagt
     6421 ggcgcgcgtg ctggtggcgc gctacgggcc cggggcggag ccggcgcgca tggcctcgct
     6481 ggtgtgcgag gacgtcggca gcctgctgga cgccgtggca cagaccgatg cggtgtttct
     6541 gggcgccgtc gccgccgcgc gcgcggcgat cgacgccgac cggctggtcg agctgcgcat
     6601 gacgccgccg ctcgattcct gcgcgcgcta cgcctacgtg cgcctggccg gccgcaccga
     6661 agcgccggtg atgcaggtgc tgcggcgctt tgcggccgag cgcctgcgtg actgatgcgg
     6721 cttgccgatc aagaacatga cccatgcacc gtgggtcatc gaggcgcagc gaggtcatcc
     6781 gccatcctgt tgtccggccc catcaggtgt tcaccgttcg cccagagctt gtcgaagggc
     6841 ttcgacaggc tgagcccgaa cggggtcaca caagctatca actcaatagc tgccggcgct
     6901 tgccgggact gcgtcagagg cctaaaacac ttcaagcccg gcgcggcggc ctcacatcag
     6961 caggtgctcg ccggcgttgt cgccgcccag gatcacgtag ttgaccttgc gcacgtccat
     7021 cagcttgcgg ccgccggcgt agctgatcga gctctgcacg tcctgctcca tctcggtcag
     7081 ggtgtcggcc agcctgccct tgatgggctc caggatgcgc ttgccctcca cgtgcttgta
     7141 ctcgcccttg ttgaagtcgc tggccgagcc gtagtactcc ttgtactgca cgccgtcgac
     7201 ctcgaccgtg cggccgggcg actcctcgtg gccggcaaac agcgagccga tcatgatcat
     7261 ggtggcgcca aagcggatcg acttggcgat gtcgccgtgg tggcgaatgc cgccgtcggc
     7321 gacgatcggc ttggtggcca cgcgcgcgca ccacttgacg gcgctgagct gccagccacc
     7381 ggtgccaaag ccggtcttca gccgggtgat acataccttg cccggcccca cgcccacctt
     7441 ggtggcgtcg gcgccccagt tctccaggtc gatcaccgcc tcgggcgtgg ccacgttgcc
     7501 ggcaatcacg aagctggccg gcaggcgcgc cttgatgtgg gtgatcatgt cgcgcacctg
     7561 gtccgaatgg ccgtgcgcga tgtcgatggt gatgtactcg ggcgtcagcc cctcggcggc
     7621 aagctggtcg atgcaggcgt agtcggccgg cttgatgccg accgagatcg acgcgtacag
     7681 cccgcgcgcc ttcatgtcgc gcacgaaggc caggttgtcg aagtcgaagc ggtgcatgac
     7741 gtagaaatag ccatgctcgg ccagccagat gcacagcctt tcatcgacca ccgtcttcat
     7801 gttggccggc accaccggca gccggaagcg gcgtccgccg aagtcgatgc cggcatcgca
     7861 ttcgctgcgg ctttgcacgc ggcacttgcg cggcagcagc aggatgtggt cgtagtcgaa
     7921 gatttccatg gtgtccaagc tccttgtgca gtgtgacgtc gagggcgctt ggcctggcac
     7981 cgggcttttg tggcaggcac aaaaaaccgg gccaagaaaa cttgggcccg gtgcttgatt
     8041 ctagtgcagt gctgcaggcc gcgctcaatc cacccacgcc acccagccca tccaccaggt
     8101 cagcagcacc acggcgccga acgccaggcg ataccaggca aagggcacga aactgtggct
     8161 ggcgatgtag cgcagcagcc agcgcacgca gatccaggcg ctgagaaacg aaaacagcag
     8221 accgatgccg aacagcggca ggtcggccca ggacaacagg gcgcgctcct tccagagcga
     8281 ataggcaccg gcgccgatca gcgtcggaat cgccaggaag aacgaaaaat cggtcgccgc
     8341 ctggcgcgac agccccagca gcatgccgcc gatgatggtg gcgcccgagc ggctggtgcc
     8401 cgggatcatg gcaaagcact gcaccaggcc gaccttgagc gcatccagcg ccgtcatgtc
     8461 gtccacccgg tgcacgcgcg cggcgctttg cggccggcgc tcggcccaca ggatgacgaa
     8521 ggcgcccagg atgaaagtgc tggccaccac ctgcggcgtg aacaggtgcg ccttgatcgc
     8581 cttgccgaac agcaggccca gcagcaccgc cggtacgaag gcgatgacga cgttgagggc
     8641 aaagcgctgc gcctgccggc tgcggggcag ctcgaccagc gtggcgcgaa tcttgtgcca
     8701 atagacgatc accaccgcca gaatggcgcc ggtctggatg gcgatctcga acaccttgac
     8761 cttgtcgccg gtcaagccca gcaggctgcc ggccagaatc aggtggccgg tgctggagat
     8821 gggcagaaac tcggtcagac cttcgaccac gcccatgagc gccgccttga ccatcaacag
     8881 cacatccact caccactcct tttcgggtca actttgctac accatttgta gctgacagcg
     8941 cacattctac aagcgctgga agctgatttt ttccggaaac gcgaatggcc ggcggcgtgc
     9001 gggcgagcgc cgcattccgt cggcgcgctg cgcacctgcc gccggccggc ggcatttcgt
     9061 cgcatcatgc tggcgcgccg tggctggcgg cggcacagtg gcgccatcgc agcaaggagc
     9121 agcaccatga acgtccccca cacctcctac acccccgagc agatcgaccg gctggcgcgc
     9181 aggcgcgccg gtgccaagat gggctggttc atccacgcct ttgtctacct gtgcgtcaac
     9241 gccggcctgg ccctgctggc gctgagccat gggcgcgact ggtacgccta cccgctgctg
     9301 ggctggggcc tggggctggc gctgcatggc gcggccgtgt ggctgatggg gccggg
//
LOCUS       DAOQTZ010000099         9251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_8327_length_9251_cov_12.989017, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000099 DAOQTZ010000000
VERSION     DAOQTZ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8327_length_9251_cov_12.989017"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PKN51_10925"
     CDS             complement(<1..553)
                     /locus_tag="PKN51_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNT85654.1"
                     /translation="MKLLFVADPIESFKTYKDTTFTMMREAARRGHTLAACEPRQLRW
                     QTGQPVQAELREIALTGEDGPWFAERGRRVAALREFDAVLMRKDPPFDAEFTYATHLL
                     EQAERDGARVINRPAALRNHPEKLAIMEWPQFIGPTLVTREPEAVRRFHAEHGEIILK
                     PLDGMGGMGIFRVGPDGLNLGSIT"
     gene            643..1845
                     /locus_tag="PKN51_10930"
     CDS             643..1845
                     /locus_tag="PKN51_10930"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNT85655.1"
                     /translation="MQAVSPAALAGVRVIEMGQLIAGPFAGKTLGDFGAEVIKIEPPG
                     RGDPLRGWRLLKAGTSVWWQVQSRNKRSLALDLRQPEGQALARQLVAEADVLIENFRP
                     GTLEGWDMAPAQLHTLNPGLVILRVSGYGQSGPYRDLPGFGVVGEAMGGLRHLTAEPG
                     RVPVRVGVSIGDTLAALHGVIGVLLALYHRKAHGGPGQVIDVALHEAVFNCMESLIPE
                     YSAFGTVRQPAGSALPGIAPSNAYPCQDGWVLVAGNGDSIFKRLMQAIGREDLAAAPD
                     LADNAGRVARVDEIDAAIGAWTEPRTVQAVLDALAAARVPAGKVYTARDIAEDPHYRA
                     REMIVAQPTRDGDVVEVPGVVPKLSVTPGTLRRSAPHLGDDTDAVLRELGLDAAQIAA
                     LRQRGIVQ"
     gene            complement(1928..3799)
                     /locus_tag="PKN51_10935"
     CDS             complement(1928..3799)
                     /locus_tag="PKN51_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNT85656.1"
                     /translation="MSASKSSLASLTLGAIGVVYGDIGTSVLYTLKEVFGSGHVPFTT
                     DNVYGVLSIVFWTLTIIVSLKYVTLVLRADNHGEGGLVAMLALASQSVKHKAPALRKW
                     MLLIGIFGTCLFYGDGVITPAITVLSAVEGLDVVSPAFRKAVIPLTLLILLALFWVQK
                     RGTAGIGRFFGPIMVLWFIAIAALGVWHIASHPEILAALSPHYALGFIWRQPGTAFII
                     LGAVVLCVTGGEALYADMGHFGKRPIRVAWFSIVMPCLTLNYFGQGALLLSEPAAVKN
                     PFFHMAPDWLTLPLVGLATMAAVIASQALITGAFSVTKQVIQLGYLPRLQIQHTSTRD
                     TGQIYMPFVNWSLFVMIVLAVVLFRSSSNLAAAYGIAVTLDMTITTVLTFFVIRYGWG
                     YPLALCIAATGFFFVIDLAFFSSNLLKLAEGGWFPILIGGAVFTLMVTWKDGRELLNQ
                     SLRDSSIDLKSFIDSVFLSPPTRVPGTAVFLSAEPGIVPNALMHNLKHNKVLHAQNLF
                     VTRRAHEVPWIGLDKRLEVQALGSDCWQVVVHHGFKNDPDLPKALELLAGRGIDLNPM
                     TTSYFLSRDIVTPTLGSGMAFWREKLFAAMHHGASAAAEYLNLPSNAVVELGSKVEI"
     gene            complement(3860..5140)
                     /gene="gshA"
                     /locus_tag="PKN51_10940"
     CDS             complement(3860..5140)
                     /gene="gshA"
                     /locus_tag="PKN51_10940"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNT85657.1"
                     /translation="MVPHLVTALTGPINELEQRILDATPVIERWFRLEWMEHTPPLYC
                     AVDIRNAGFKLAPVDTNLYPDGWHNLSPDTLPLAVQAAQAAIEKICPEARNLLIVPEN
                     GKPSSFYLASLQRLQEIFRMAGLNVRLGSIDPTLKRSTTLTLPDGDKLTLEPAQRTRF
                     RLGLKHFDPCTILLNNDLAAGAPGILEDLPEQYLLPPLQAGWSVRRKSRHFHSYDEVS
                     KRFGKLLGVDPWLINPLFATCGEIDIHAPDGGECLRSHVDALLTKIRRKYKEYGIAEK
                     PFVVVKADQGTRGMGIMTVRDLKDLAGLDRPGRPAPDLPRGEVIIQEGVLTNERIHDA
                     VAEPVVYLIDRYVVGGFYRIHAERGVDENLNAPGARFVPLPFAGNAHLPRPGAKGEAR
                     PPNRFYMYGVIARLAMVAASYELEATDPELQALA"
     gene            complement(5318..7174)
                     /locus_tag="PKN51_10945"
     CDS             complement(5318..7174)
                     /locus_tag="PKN51_10945"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNT85658.1"
                     /translation="MPATLPFWLRLLLAAALSLAGLAVAAAPQRVTQAMATLTPDGGP
                     PQTQEITLPHRWDDSFPGRGGRATYQFELPATPDPSPRALYLPRAGNQIEVWLNDRLI
                     HRVGRLGDQRTDAAKAPHWIPLPEVAHASGQPAARVQVRLSAQAARWGGLSAPWVGPR
                     DELYARYREHYRLRQYGGLIVCAVLGLMALMAAGFWWVQRDRLYLIFCLASAAGVLRI
                     ADRLIEHPPLPWPVWGLVTAWAMVALMVLMVYFALMLVNRQQLLDAAWFRLLVGLDLL
                     LTAAAYVLGLPQLWTLTLTSLVVPAILSWVAMGQAAWRQRSGQAVAVFVAGSALIGTG
                     VRDWWNVRLYGDGAGTVSLMPHALLLFALLMGAVLVQRFARVAGERRTLLTTLEARVR
                     QREVELNQRHAELRQEYAHQAALQERQRLMRDIHDGVGLQLVGLLDLLDHDATPRELL
                     RQQLGTALDELRMAVDALQPTHGDLTTVLATLRYRLQPRLQAAGIAIDWQVDELPPIE
                     GLTPTTVLQLQRVLQEAFTNALRHAQASRLWVRACLDHGPPERLVLSVEDDGRGLPDN
                     GGGAAPAGGQGLSSMQARAQAIGAELSVGPGAHGGTLVRLALPAGSAVGAPP"
     gene            complement(7217..7867)
                     /locus_tag="PKN51_10950"
     CDS             complement(7217..7867)
                     /locus_tag="PKN51_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT85659.1"
                     /translation="MWAEPPISVALVEDDAATRARLSHVLAAAPGIALVHTAASAREM
                     LDWLAGNSVNVLLVDLGLPDRSGLEVIRWCSTHQPATNVMVVTLFGDEAHMIGAFEAG
                     ARGYLLKDGTEDELARHVLSLHSGGSPMTPLIARQLLARLTSPARPPAPASQPISARE
                     KDILQALSRGYTYDEAARLLGIAPSTVQSHVKNIYSKLAVHSKTEAIFEARQLGLL"
     gene            8162..>9251
                     /locus_tag="PKN51_10955"
     CDS             8162..>9251
                     /locus_tag="PKN51_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85660.1"
                     /translation="MPRSNQKMQMLSALCAATLLTLTACGGDGDDSVAGPAPAPSPAP
                     GPAPVTLQGQVTRNAALKDATVCLDLNGNDACDAGEPASALTGEDGRYSLTALPDQAA
                     AARLIAVVKANVTTDASNPGQPVTTTTDYVLKRPAGSAGGINPLTTLVQAGVAAGMTE
                     AQARSNAAAQLGIDAAKIDDYQDDPPASDTQVQDTARWIAAFTSIALRDGIPLEVADP
                     AVAEPASDVMDNLIWADASNFYWRSRQQAARPAGAPTPAVADMRAGKIGGTARPDFGA
                     ANSLYRTAYLTPAGWQMCGRNTPPIVSSGGNPSRSLYCGTSAAVTIHRRSDVAGEAMS
                     ALVTRWQGDAVTNRINNDGTPVAALNGAL"
BASE COUNT         1425 a         3191 c         3317 g         1318 t
ORIGIN      
        1 cggtgatcga gcccaggttc agcccgtccg ggccgacgcg gaagatgccc atgccaccca
       61 tgccgtcgag cggcttgagg atgatctcgc catgttcggc gtgaaagcgc cgcaccgcct
      121 cgggctcgcg cgtcaccagc gtcgggccga tgaactgcgg ccactccatg atggccagct
      181 tttccgggtg gttgcgcagc gccgccggcc ggttgatgac gcgcgcgccg tcgcgttcgg
      241 cctgctccag caggtgggtg gcgtaggtga attcggcgtc gaacggcggg tccttgcgca
      301 tcagcacggc gtcgaactcg cgcagcgccg ccacgcgccg gccgcgctcg gcgaaccagg
      361 ggccgtcctc gcccgtgagg gcgatctcgc gcagctcggc ctgcaccggc tggccggtct
      421 gccagcgcag ctggcgcggc tcgcaggcgg ccagcgtgtg gccgcgccgg gcggcttcgc
      481 gcatcatggt gaaggtggtg tccttgtagg tcttgaagga ctcgatcggg tcggcgacga
      541 acagcagctt catggcggcg gcggcgggcg gcggtgaaag ggccatactg ttgcacaaac
      601 caccgcgcgg cgtcggccgc gccccgatct gacgaggtcc gcatgcaagc agtttccccc
      661 gccgccctgg ccggcgtgcg cgtgatcgag atgggccagc tgatcgccgg gccgtttgcc
      721 ggcaagacgc tgggcgactt cggcgccgag gtgatcaaga tcgagccgcc cggccggggc
      781 gatccgctgc gcggctggcg cctgctcaag gcaggcacct cggtgtggtg gcaggtgcag
      841 tcgcgcaaca agcggtcgct ggcgctggat ctgcgccagc ccgagggaca ggcgctggcg
      901 cgccagctgg tcgccgaggc cgacgtgctg atcgagaact tccgccccgg cacgctggag
      961 ggctgggaca tggcgccggc gcagctgcac acgctcaatc cggggctggt gatcctgcgc
     1021 gtgtcgggct acggccagag cgggccgtac cgcgacctgc cgggctttgg cgtggtgggc
     1081 gaggccatgg gcgggctgcg ccacctgacg gccgagcccg gccgcgtgcc ggtgcgcgtg
     1141 ggggtgtcga tcggcgacac gctggcggcg ctgcacggcg tgatcggggt gctgctggcg
     1201 ctctaccacc gcaaggcgca cggcgggccg gggcaggtga tcgacgtcgc cctgcacgaa
     1261 gcggtgttca actgcatgga aagcctgatc cccgagtaca gcgccttcgg caccgtgcgc
     1321 cagccggccg gcagcgccct gccgggcatc gcgccgtcca acgcctaccc ctgccaggac
     1381 ggctgggtgc tggtggccgg caacggcgac agcatcttca agcgcctgat gcaggccatc
     1441 ggccgcgaag acctggccgc tgcccccgac ctggccgaca acgccggccg ggtggcgcgc
     1501 gtggacgaaa tcgatgccgc catcggcgcc tggaccgagc cgcgcaccgt gcaggcggtg
     1561 ctggacgcgc tggcggcggc gcgtgtgccg gccggcaagg tgtacaccgc gcgcgacatc
     1621 gccgaagacc cgcactaccg cgcgcgcgag atgatcgtgg cgcagcccac gcgcgacggc
     1681 gatgtggtcg aggtgcccgg cgtggtgccc aagctgtcgg tcacgcccgg caccctgcgc
     1741 cgcagcgccc cccacctggg cgacgacacc gacgccgtgc tgcgcgagct gggcctggac
     1801 gcggcgcaga tcgcggcgct gcgccagcgt ggcatcgtgc agtgaaccgg gacacggcgg
     1861 ctcacagaat gctatataaa taatagctgt tggcgctttc ctggcaagcg ctgggagttg
     1921 ttttggctca gatctcgacc ttgctgccca gctcgaccac ggcgttgctg ggcaggttca
     1981 ggtactcggc cgccgccgag gcgccgtggt gcatggcggc gaacagtttt tcgcgccaga
     2041 acgccatgcc cgagccgagg gtcggcgtga cgatgtcgcg cgacaggaag tagctggtgg
     2101 tcatggggtt gaggtcgatg ccgcggccgg ccagcagctc cagcgccttg ggcaggtcgg
     2161 ggtcgttctt gaagccgtgg tgcaccacca cctgccagca gtcgctgccc agcgcctgca
     2221 cctccagccg cttgtccagc ccgatccagg gcacctcgtg cgcgcgccgg gtgacgaaca
     2281 ggttctgcgc gtgcagcacc ttgttgtgct tgaggttgtg catcagcgcg ttgggcacga
     2341 tgccgggttc ggcgctcagg aacaccgcgg tgcccggcac acgcgtgggc gggctgagga
     2401 acaccgagtc gatgaaagat ttcaggtcga tcgagctgtc gcgcaagctt tggttgagca
     2461 gctcgcggcc gtccttccag gtcaccatca gggtgaacac cgcgccgccg atcaggatcg
     2521 ggaaccagcc gccctcggcc agcttgagca ggttggagct gaagaacgcc aggtcgatga
     2581 cgaagaagaa accggtggcc gcgatgcaca gcgccagcgg gtagccccag ccgtagcgga
     2641 tgacgaagaa ggtcagcacg gtggtgatgg tcatgtccag cgtcaccgcg atgccatagg
     2701 ccgccgccag gttgctcgac gaccgaaaca gcaccacggc cagcacgatc atcacgaaca
     2761 ggctccagtt gacgaagggc atgtagatct ggccggtgtc gcgcgtgctg gtgtgctgga
     2821 tctgcaggcg cggcaggtag cccagctgaa tcacctgctt ggtgacgctg aaggcgccgg
     2881 tgatcagcgc ctgcgaggcg atcaccgccg ccatcgtggc cagcccgacc agcggcagcg
     2941 tcagccagtc gggcgccatg tggaagaacg ggttcttcac cgccgcgggc tcggacagca
     3001 gcagcgcgcc ctggccgaag tagttcagcg tcaggcaggg catgacgatg gagaaccaag
     3061 ccacgcggat cggccgcttg ccgaaatggc ccatgtcggc gtacagcgcc tcgccgccgg
     3121 tgacgcacag caccaccgcg cccaggatga tgaaggcggt gcccggctgg cgccagatga
     3181 agcccagcgc gtagtgcggg ctgagcgcgg ccaggatttc cgggtggctg gcgatgtgcc
     3241 agacaccgag cgctgcgatg gcgatgaacc acagcaccat gatcggcccg aagaagcgcc
     3301 cgatgccggc cgtgccgcgc ttttgcaccc agaacagcgc cagcagaatc agcagcgtga
     3361 gcgggatcac ggccttgcgg aacgccggcg acaccacgtc cagcccctcc accgccgaca
     3421 gcaccgtgat ggccggggtg atgacgccgt cgccgtagaa caggcaggtg ccgaagatgc
     3481 cgatcagcag catccacttg cgcagcgccg gcgccttgtg cttgaccgac tgcgaggcca
     3541 gcgccagcat ggccaccagg ccgccctcgc cgtggttgtc ggcgcgcagc accagggtga
     3601 cgtatttcag cgagacgatg atcgtcagcg tccagaacac gatcgacagc acgccgtaga
     3661 cgttgtcggt cgtgaacggc acgtggccgc tgccgaacac ctccttgagc gtgtacagca
     3721 cgctggtgcc gatgtcgcca tacaccacgc cgatggcacc cagcgtcagg ctggcgaggg
     3781 aagacttgga agccgacatg gcgtagggat gttcttgcgg gagcgggatg gtgcgcccgc
     3841 cgcggtggtg cggtgcgcct caggccaggg cctgcagctc cgggtcggtg gcttccagct
     3901 cgtagctggc ggccaccatg gccaggcggg cgatcacgcc gtacatgtag aagcggttgg
     3961 gcgggcgcgc ctcgcccttg gcgcccgggc gcggcaggtg ggcgttgccg gcaaacggca
     4021 gcggcacgaa gcgcgcgcca ggggcgttga ggttctcgtc caccccgcgt tcggcgtgga
     4081 tgcggtagaa gccccccacc acgtagcggt cgatcaggta caccaccggc tcggccaccg
     4141 cgtcgtggat gcgctcgttg gtcagcacgc cctcctggat gatgacttcg ccgcgtggca
     4201 ggtccggcgc cggccggccg gggcggtcga gcccggccag atccttgagg tcgcgcaccg
     4261 tcatgatgcc catgccgcgc gtgccctggt cggccttgac gaccacgaac ggcttttcgg
     4321 cgatgccgta ttccttgtac ttgcggcgga tcttggtcag cagcgcatcg acgtggctgc
     4381 gcaggcactc gccgccgtcg ggcgcgtgga tgtcgatctc gccgcaggtg gcgaacagcg
     4441 ggttgatgag ccaggggtcg acccccagca gcttgccgaa gcgcttgctc acttcgtcgt
     4501 agctgtggaa gtggcggctc ttgcggcgca cgctccagcc ggcctgcagg ggcggcagca
     4561 ggtattgctc gggcaggtct tccaggatgc ccggggcgcc ggccgcaagg tcgttgttga
     4621 gcaggatggt gcaggggtcg aagtgcttca gccccaggcg aaagcgcgtg cgctgggccg
     4681 gctccagcgt cagcttgtcg ccgtcgggca gggtcagggt ggtgctgcgc ttgagcgtcg
     4741 ggtcgatcga gcccaggcgc acgttcagtc ccgccatgcg gaagatctcc tgcagccgct
     4801 gcaggctggc caggtagaag ctggagggtt tgccgttttc cggcacgatc agcaggttgc
     4861 gcgcctcggg gcagatcttc tcgatcgccg cctgcgccgc ctgcaccgcc agcggcaggg
     4921 tgtcgggcga caggttgtgc cagccgtcgg ggtagaggtt ggtgtccacc ggcgccagct
     4981 tgaagccggc gttgcggatg tcgaccgcgc aatacagcgg cggcgtgtgc tccatccact
     5041 ccagccggaa ccagcgctcg atcaccggcg tggcgtcgag gatgcgctgc tccagctcgt
     5101 tgatggggcc ggtcagtgcg gtgacgagat ggggaaccat ggtcgagagg gcgtgcggat
     5161 agccgcagtg taagggcgtt gcggcgcccg ccgatgccct gcgcggactc ggtgtgcaca
     5221 aaaagaacta tcgattcaat agctgctggc gctggtccag agggtgtgaa acccctgttt
     5281 gagcaccaaa aacgcacccc cgggcgggcg cccagcgtca gggcggcgcg cccaccgcgc
     5341 tgcccgccgg cagcgccagg cgcaccaggg tgcccccatg cgcgccgggg ccgacgctca
     5401 gctcggcgcc gatggcctgg gcgcgtgcct gcatgctgct cagcccctgg ccgcctgccg
     5461 gcgcggcgcc gccgccgttg tcgggcaggc cgcggccgtc gtcctccaca ctcagcacca
     5521 gacgttcggg cgggccgtgg tccaggcagg cgcgcaccca caggcggctg gcctgggcat
     5581 ggcgcaaggc gttggtgaag gcctcctgca gcacgcgctg cagttgcagc accgtggtcg
     5641 gggtcaggcc ctcgatgggt ggcaactcgt ccacctgcca gtcgatggcg atgccggccg
     5701 cctgcaggcg cggttgcagg cggtagcgca gcgtggccag caccgtcgtc aggtcaccgt
     5761 gcgtgggttg cagcgcgtcc accgccatgc gcagctcgtc cagtgccgtg cccagctgct
     5821 ggcgcagcag ctcgcgcggc gtcgcgtcgt ggtccagcag gtcgagcagg cccaccagtt
     5881 gcaagccgac gccgtcatgg atgtcgcgca tcaggcgctg acgctcctgc agcgcggcct
     5941 ggtgggcgta ctcctggcgc agttcggcgt ggcgctggtt cagttcgact tcgcgctggc
     6001 gcacgcgcgc ctccagcgtg gtcagcagcg tgcgtcgttc gcccgccacg cgcgcaaagc
     6061 gctgcaccag cacggcgccc atcagcagcg cgaacagcag cagcgcatgc ggcatcaacg
     6121 agaccgtgcc ggcgccgtcg ccatacagac gcacgttcca ccagtcacgg acccccgtgc
     6181 ctatcagcgc cgagccggcc acgaacaccg ccaccgcctg gccgctgcgc tggcgccagg
     6241 ccgcctgtcc catggcgacc caggacagga tggctggcac caccagcgag gtcagcgtca
     6301 acgtccacag ctggggcagt cccaagacat acgccgctgc agtgagcagc agatccagac
     6361 cgaccagcag gcgaaaccag gctgcgtcca gcagctgctg gcggttcacc agcatcaggg
     6421 caaagtagac catcaggacc atgagggcaa ccatggccca ggccgtcacc aggccccaca
     6481 cgggccaggg cagaggaggg tgttcgatca gccggtcagc gatgcgcagc acgccggccg
     6541 cggacgccag gcagaaaatc aggtacagac ggtcgcgctg cacccaccag aagccggccg
     6601 ccatcagcgc catcaggccc agcaccgcgc agacgatcag accaccgtac tgacgcagcc
     6661 gatagtgttc gcggtagcgc gcgtacagct cgtcgcgcgg ccccacccag ggtgccgaca
     6721 gcccgcccca gcgcgccgcc tgcgcgctca gccgcacctg cacgcgcgcc gccggctggc
     6781 cggaggcgtg tgcgacctcg ggcaggggta tccagtgcgg ggccttggcc gcatcggtgc
     6841 gctggtcgcc caagcggccg acgcgatgaa tcagccgatc gttcagccat acctcgatct
     6901 gattgcccgc gcgcggcagg tacagcgcgc gcgggctggg gtcgggcgtg gcgggcagct
     6961 cgaactggta ggtggcgcgc ccgccccgcc cgggaaagct gtcgtcccag cggtgcggca
     7021 gcgtgatctc ctgcgtctgc ggtgggccgc cgtccggtgt cagcgtggcc atggcctgcg
     7081 tcacgcgctg gggcgccgcg gccaccgcca gcccggccag gctgagcgcg gcggccagca
     7141 gcagacgcaa ccagaagggc agggtggcgg gcatgggcgg ggcgcggctg gctgggggtc
     7201 ggtgcccgcc agcctatcag agcaggccca gctggcgcgc ctcgaagatc gcctcggtct
     7261 tggaatgcac ggccagcttg ctgtagatgt tcttcacgtg cgactgcacg gtcgagggcg
     7321 cgatgcccag caggcgcgcg gcttcgtcgt aggtgtagcc gcgcgacagc gcctgcagga
     7381 tgtccttctc gcgcgccgag atcggctgcg acgccggcgc cggcgggcgg gccgggctgg
     7441 tcaggcgcgc cagcagctgg cgcgcgatca gcggcgtcat gggcgagccg cccgaatgca
     7501 ggctgagcac gtggcgcgcc agctcgtcct cggtgccgtc cttgagcagg tagccgcgcg
     7561 cgccggcctc gaaggcgccg atcatgtggg cttcgtcacc gaacagggtg accaccatca
     7621 cgttggtggc cggctggtgg gtgctgcacc agcggatcac ttccagcccc gagcggtcgg
     7681 gcaggcccag gtccaccagc agcacgttga cgctgttgcc ggccagccag tccagcatct
     7741 cgcgggcgct ggccgccgtg tgcaccaggg cgatgcccgg cgccgccgcc agcacatggc
     7801 tcaggcgtgc gcgcgtggcc gcatcgtcct cgaccagggc caccgaaatg gggggctcag
     7861 cccacatggg cgcacctgcg cgcgctcgtc ggcgcggatc ggccaggcac cacagcgcgg
     7921 gtccatcgct ggggcgggcg aggcggggca aagtggttca tggcgggtgg cagtgcaggt
     7981 gcgaacggcg cgcgggcatg caagcgtatg cgggttgaac cgcacgcaca agccggccgg
     8041 tatcccatgt tcatggcatg tgcgtcacgc agtccggttc gatagttcgg ccgtccgccc
     8101 ggcctgtcct atggcggcgg atggcacctt gacccacacc ctttcggagg aggaacccca
     8161 catgccccgt tccaaccaga agatgcagat gctcagcgca ctgtgcgcgg ccacgctgct
     8221 gaccctcacc gcatgcggtg gcgatggcga cgattcggtc gccggccccg cgccagcgcc
     8281 gagtcctgcc ccagggccag cccctgtgac gctgcaaggc caggtcactc gcaacgccgc
     8341 gctgaaggac gccacggtct gcctggacct caacggcaac gacgcctgcg acgccggcga
     8401 gccggcttct gccctgacgg gggaggacgg caggtattca ctcaccgcac tgcccgatca
     8461 agcggccgcc gcgcgcctga tcgccgtggt caaggccaac gtcaccaccg acgccagcaa
     8521 ccccggccag cccgtgacca ccaccaccga ctacgtgctc aagcgcccgg ccggctcggc
     8581 tggcggcatc aacccgctca ccaccctggt gcaggccggt gtggccgccg gcatgaccga
     8641 ggcgcaggcg cgcagcaacg cggcggctca gttgggcatc gacgccgcca agatcgacga
     8701 ctaccaggac gatccgcccg ccagcgacac ccaggtgcag gacacggcgc gctggatcgc
     8761 cgccttcacc tcgatcgcgc tgcgcgacgg cattccgctg gaggtcgccg acccggcggt
     8821 ggccgagccc gcctccgacg tgatggacaa cctgatctgg gccgacgcca gcaacttcta
     8881 ctggcgcagc cggcagcagg ccgcccggcc ggccggcgcg ccgaccccgg cggtggccga
     8941 catgcgcgcc ggcaagatcg gcggcaccgc ccggcccgac ttcggcgccg ccaacagcct
     9001 gtaccgcacg gcctacctca cgcccgccgg ctggcagatg tgcgggcgca acacgccgcc
     9061 cattgtctcc agcggcggca atccaagccg ctcgctgtac tgcggcacct cggccgccgt
     9121 cacgatccac cggcgcagtg acgttgccgg cgaggccatg tcggcgctgg tgacgcgctg
     9181 gcagggcgat gcggtgacca accgcatcaa caacgacggc acgccggtcg ccgcgctcaa
     9241 tggcgcgctg g
//
LOCUS       DAOQTZ010000100         8785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_8881_length_8785_cov_14.018328, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000100 DAOQTZ010000000
VERSION     DAOQTZ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8881_length_8785_cov_14.018328"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="PKN51_10960"
     CDS             complement(<1..94)
                     /locus_tag="PKN51_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNT85661.1"
                     /translation="MALKSTIFKADLQLADIDHGCYTDLQLTLAR"
     gene            complement(134..370)
                     /locus_tag="PKN51_10965"
     CDS             complement(134..370)
                     /locus_tag="PKN51_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85662.1"
                     /translation="MMLEPPPHRLPGPSYAKPLIDEQGEVRELTAADLKRMRPAALAL
                     PASLQAKLRTRGPQKTPTKQRIIIRLSPESASGA"
     gene            complement(422..1825)
                     /gene="purB"
                     /locus_tag="PKN51_10970"
     CDS             complement(422..1825)
                     /gene="purB"
                     /locus_tag="PKN51_10970"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNT85663.1"
                     /translation="MELSPLTAVSPLDGRYAAKLTPLRPLMSELGYMRYRVQVELTWF
                     VALSDAGFAEFPPLSAAARAHLRGLLANFSETDGAAIKAIERTTNHDVKAVEYWIKSK
                     FEGQPELQAATEFVHFACTSEDINNTSHALQLQAGRAVLLQGLDAVIERLRALAHQHA
                     QVPMLSRTHGQTASPTTVGKELANVVVRLQAARQRIAGVALLAKMNGAVGNYNAHLAA
                     WPDFDWEAFARRVVEAPAPEADHGFSQAFGLGLTFQPYSIQIEPHDYMAELFDAIARA
                     GTICIDLARDVWGYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENAEGNLGLANALLR
                     HLAEKLPISRWQRDLTDSTVLRNMGVALGYAVLAYQSLLTGLNKLELNESQLAADLDH
                     AWEVLAEPIQTVMRRYGVPGAYEKLKEVTRGQQVTREALHGLIRGLDIPAAEKERLLA
                     LTPAGYVGKAGELAGRV"
     gene            2019..2639
                     /locus_tag="PKN51_10975"
     CDS             2019..2639
                     /locus_tag="PKN51_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNT85664.1"
                     /translation="MRLIGATTSPYVRKVRAVMAEKKLDYQFVAENVWADDTQIGASN
                     PLGKVPCLVMDGQDAVFDSRVIVEYLDTLSPVGRLIPPSGRQRVEVKTWEALADGLLD
                     AAILARLEKTWPGRAEGERSPAWIARQMKKIDDALAAMSAGLADKPWCSAGVHLSLAD
                     IAVGCALGYLDFRFPELDWRERHANLARLLDERLMPRPSFADTVPA"
     gene            complement(2931..4568)
                     /locus_tag="PKN51_10980"
     CDS             complement(2931..4568)
                     /locus_tag="PKN51_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT85665.1"
                     /translation="MSPGWRRRNWLAAGAGALLGAACTRDAAQAIEGGFANDSDARGH
                     RLRQPWPTRAPDLTRHVHTVIAGGGIAGLAAARALRLAGHDDFALLELEDTAGGHSRG
                     MQVNGIACPMGAHYLPLPGDDARAVQDLLEELGLRRRVAGRWQYDERALCHSPQERLF
                     LHGQWQDGLLPLQGVGGATLAQYRRFAAAVASAQAAAHYAIPVQKSSFSPVQLAQDAI
                     TFEAWLNQQGFHDRQLRWYLDYCCRDDYGAGSASVSAWAGLHYFASRHGFAAPGDAAH
                     DDAGVLTWPEGNGWLAAQLAAPLAERLQPARVVGRIEHGRHGVTLDALDIRSGRLERW
                     QARQAVIALPVWVAARVVEAPPEALRQRAARLRSAPWLLAQLKLRAPLRERPGAAPSW
                     DNVLYGAPGLGYVNAMHQSLHPVPGATVLTYYRALGDAPDGRQRLLDTPWTAWRDAIL
                     AELGAAHPDLAERTERMALLRHGHAMAVPTPGTLSQIERLRASRKRQQLSNSEHAGWV
                     FDAAPRLAFAHADWSGYSVFEEAFTLGHAAGTALAGR"
     gene            complement(4565..6175)
                     /locus_tag="PKN51_10985"
     CDS             complement(4565..6175)
                     /locus_tag="PKN51_10985"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNT85666.1"
                     /translation="MTASSSTLVAPTGAAPRPMAVALLASVFVIAACGLLYELAAGAL
                     ASYLLGDSVLQFSTVIGTYLFAMGVGSWLSRYFERQLPAHFLRIELLIALFGGALPAL
                     LFVANATVPGAFRPLLYGMVLVVGTLVGLEIPLVMRILKRDVTIRDLVSQVLTFDYLG
                     ALAVSLAFPLVLVPRLGLIRTGFLFGLMNAAVAIWALWLFRHGLPRRRAFAVASAVVV
                     ALLAAGMAGAGRITSLAEEHFYQAPITFAESTPYQRIVVTGGHGGLRLFLNGNLQFAQ
                     RDEYRYHEALVHPAMAAHGAPRRVAVLGGGDGMAVREVLKYPSVESVTLVELDPAMTQ
                     LFRDTPQLAQLNHGALRDPRVQIVNTDAFQWLQQGVAPTAQEPAAADPLAARGGEAFY
                     DVIVVDFPDPTNFSIGKLYTLSFYALLEQRLAASGYAVIQTTSPLVARRSFWTVVQTV
                     EAAGLSATPYHAHVPSFGEWGFIVAGRRPWRLPGTLPDGLRFLTPHTLPLLFDFPADM
                     ARVPAEVNRLSNQALVHTYETEWGRVQE"
     gene            complement(6231..6449)
                     /locus_tag="PKN51_10990"
     CDS             complement(6231..6449)
                     /locus_tag="PKN51_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNT85667.1"
                     /translation="MDAEWLKPAALIGSLVYALIGVAIFWLCFVIIDKLTPVNLWQEI
                     VEKHNRALALVVAAMCLGISIIVAAAIH"
     gene            complement(6477..7973)
                     /locus_tag="PKN51_10995"
     CDS             complement(6477..7973)
                     /locus_tag="PKN51_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HNT85668.1"
                     /translation="MATDAPQRHYRAACPSCGAPVEFQSATAPFAVCGFCRSTVVRSG
                     EQLQRIGQMAELFDDHSPLQLMASGRVTLDGNKLPFTLIGRLQFKSDSGTWTEWIAAL
                     DDGRTGTLSEDNGSYVFTRPDEGTEPPPAQPLPAAGSSLRLGVTDWVVAYSGSAQLIA
                     AEGELPKLPALGQPFKLLELRSEDGQVLSIDYGAAPPQVERGRAVELAHLQLKGLKDA
                     SVREDQGGRSFACPNCGAPVHVQLDNTKRCTCAQCESLIDLTAGVGGELLAAKQKVRV
                     PPLIPLGSRGQLQGTSWQVVGWQRRSGREPGDDEKFEWKEYLLYHRERGFAFLVDTED
                     GWSLVFTATGAPRIDQGKRRARYQGSTYTLQSAYEATTTEVLGEFYWPVERGQKTDNH
                     DYANGNKLLSTERSNGEVTASVGSRIDGAAVAEAFGMSQRKALFERAVIGPAELAAEG
                     MTQKIIMAIVVIIVISTVLDSCSLELDDYLARGTGGAFGGYSSGGSHK"
     gene            complement(8040..>8785)
                     /locus_tag="PKN51_11000"
     CDS             complement(8040..>8785)
                     /locus_tag="PKN51_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNT85669.1"
                     /translation="YFFSTRQQVDQKWGTPQPISIRDKEFGAVRLRAFGNYAYRVADP
                     KKFHTEISGTRDVYAVDDLDGQLRGLITQHIATGIAQSGIPFLDLAANQQAFAQALSN
                     ALAPAMEAYGLKLEGMTVQSISLPEELQKVLDQRIGMGMVGGDMGKFMQYQTAQAIPK
                     MAEGVGAGGDAGGLAGAGLGMGAGVAMGQILAQNMQSALQGGATPAAGVRPEDVMATL
                     EKLGELKAKGILTEDEFNAKKAELLKKLV"
BASE COUNT         1325 a         2947 c         3142 g         1371 t
ORIGIN      
        1 ggcgcgccag ggtcagctgc aggtcggtgt agcagccgtg gtcgatgtcg gccagctgca
       61 gatcggcctt gaagatggtg gatttgaggg ccacgggatg actatgaatt ggatagctgt
      121 tggcgcattc tggctaagcg ccagaggctg attccggtga caggcgaatg atgatgcgct
      181 gcttggtcgg ggttttctgc gggccgcggg tgcgcagttt ggcttgcagc gacgcgggca
      241 aggccagggc agcggggcgc atgcgcttga ggtcggcggc ggtgagttcg cggacctcgc
      301 cctgctcatc gatcaggggt ttggcataag aggggcctgg caggcgatgg ggcggcggtt
      361 caagcatcat ggtcttcgac aatcccggaa tcactccggc cactcatcgg cccaccgcga
      421 gtcacacccg ccccgccaat tcccccgcct tgcccacata cccggccggc gtcagcgcca
      481 gcagccgctc cttttcggcg gccggaatgt ccagcccgcg gatcaggccg tgcagcgctt
      541 cgcgcgtgac ctgctggccg cgggtgactt ccttgagctt ttcgtacgcg ccgggcacgc
      601 cgtagcggcg catcacggtc tggatgggtt cggccagcac ttcccaggcg tggtccaggt
      661 cggcggcgag ctgcgactcg ttgagttcca gcttgttcag gcccgtcagc aacgactggt
      721 aggccagcac ggcgtagccc agggccacgc ccatgttgcg cagcacggtg gagtcggtca
      781 ggtcgcgctg ccagcggctg atgggcagct tttcggccag gtggcgcagc agggcgttgg
      841 ccaggcccag gttgccttcg gcgttctcga agtcgatggg gttgaccttg tgcggcatgg
      901 tgctggagcc aatctcgccc gccttggtct tttgcttgaa gtagcccagg ctgatgtagc
      961 cccacacgtc gcgcgccagg tcgatgcaga tggtgcccgc acgggcgatg gcgtcgaaca
     1021 gctcggccat gtagtcgtgc ggctcgatct ggatgctgta gggctgaaac gtcaggccca
     1081 ggccaaaagc ttgagagaag ccgtgatcgg cttcgggtgc cggcgcctcg accacgcggc
     1141 gggcaaaggc ctcccagtcg aagtcgggcc aggcggccag gtgggcgttg tagttgccca
     1201 cggcgccgtt catcttggcc agcagggcca caccggcgat gcgctggcgc gcggcctgca
     1261 gtcgcaccac cacgttggcc agctccttgc cgacggtggt ggggctggcc gtctgcccgt
     1321 gcgtgcggct gagcatgggc acctgggcgt gctggtgggc cagcgcgcgc aggcgctcga
     1381 tcacggcatc cagcccctgc agcagcacgg cgcggccggc ctgcagttgc agcgcgtggc
     1441 tggtgttgtt gatgtcctcg ctggtgcagg caaagtgcac gaactcggtg gcggcctgca
     1501 gctcgggctg cccttcgaac ttgctcttga tccagtattc gaccgccttg acgtcgtggt
     1561 tggtggtgcg ctcgatggcc ttgatggcgg cgccatcggt ttcggaaaag ttggccagca
     1621 ggccccgcag gtgggcgcgc gcggcggccg agagcggcgg aaactcggca aagccggcgt
     1681 cggacagggc cacgaaccag gtcagctcga cctgcacgcg atagcgcatg tagcccagct
     1741 cgctcatcag cgggcgcaag ggggtgagct tggcggcata gcggccgtcc agcggcgaaa
     1801 cggcggtcaa ggggctgagt tccatgccac ggattgtaag aagacgacct gccagcgttc
     1861 gtccttcttt tgcttcggaa gcctggagtt tgagccgttc aacctgagcc tgtttaaggg
     1921 cttgggcaag ctgagcccga acgggtttca agacatcccc aagccgaatc aatagaatgc
     1981 ctgcttcccg tcacatccgg aagcatctgt ccatgtccat gcgattgatc ggcgccacca
     2041 ccagcccgta cgtgcgcaag gtgcgcgcgg tgatggccga gaagaagctg gactaccagt
     2101 tcgtggccga aaacgtgtgg gccgacgaca cgcagatcgg cgcctccaac ccgctgggca
     2161 aggtgccctg cctggtgatg gacgggcagg acgcggtgtt cgattcgcgc gtgatcgtgg
     2221 agtacctgga cacgctgtcg ccggtgggcc ggctgatccc gccgtcgggc cgccagcgcg
     2281 tggaggtcaa gacctgggag gcgctggccg acggcctgct ggacgccgcc atcctggcgc
     2341 ggctggagaa aacctggccc ggccgcgccg agggcgagcg cagcccggcc tggatcgcgc
     2401 gtcagatgaa gaagatcgac gatgcgctgg ccgccatgag cgccggcctg gccgacaagc
     2461 cctggtgttc ggccggcgtg cacctgtcgc tggccgacat cgccgtgggc tgcgcgctgg
     2521 gctacctgga ctttcgcttt cccgagctgg actggcgtga gcgccacgcc aacctggcgc
     2581 gcctgctgga cgagcgcctg atgccgcgcc ccagctttgc cgacaccgtg ccggcatagg
     2641 cagcgcgccc agatgagcgc gccgccgggg cacgtggccc gggcggcgcg gtgggaataa
     2701 aaaagctgcc gaagcggcct gaaacgaaag aggaggaggg aggaggagga agtccgcctc
     2761 aggttttcag ccgactcgcg gagccggcag gctcggcagc gtcaaggcaa ttcatggagt
     2821 caatgccatg catcacgtgg attgagacgt gagtgtgcgc ccaaagttcc gcccgcgctg
     2881 ccccggcgcg cgtaaagaag tgtgaaagcc tggggtctgc gcgcagaccc tcagcgcccc
     2941 gccagcgcgg tgccggcggc gtggcccagg gtgaaggcct cttcgaacac cgagtagccc
     3001 gaccaatcgg catgggcaaa ggccagccgc ggtgcggcat cgaaaaccca tcccgcgtgc
     3061 tctgaatttg atagctgctg gcgctttctg gatgcgcgta ggcgctcgat ttggctcaaa
     3121 gtaccggggg tgggcacggc catggcgtga ccgtggcgca gcagcgccat gcgttcggtg
     3181 cgctccgcca ggtcggggtg ggccgcgccc agctcggcca ggatggcgtc gcgccaggcg
     3241 gtccagggcg tatcgagcag gcgctggcgg ccgtcgggtg cgtcgcccag tgcgcggtag
     3301 taggtcagca ccgtggcgcc gggcaccggg tgcaggcttt ggtgcatggc gttgacgtag
     3361 cccaggccgg gcgcgccgta cagcacgttg tcccagctgg gcgcggcacc ggggcgctcg
     3421 cgcagcggtg cgcgcagctt gagttgggcc agcagccagg gggcgctgcg caggcgggcg
     3481 gcgcgctggc gcagggcttc gggcggcgct tcgacgacgc gcgcggccac ccacaccggc
     3541 agggcgatca cggcctggcg cgcctgccag cgctccagcc ggccgctgcg gatgtcgagc
     3601 gcgtccagcg tcacgccgtg gcgcccgtgt tcgatgcggc cgaccacgcg cgccggctgc
     3661 aggcgctcag ccagcggcgc cgccagctgc gccgccagcc agccgttgcc ctcgggccag
     3721 gtcagcacgc cggcgtcgtc gtgcgccgcg tcgccaggcg cggcaaagcc gtgccggctg
     3781 gcaaagtagt gcagcccggc ccaggccgac acgctggcgc tgccggcacc gtagtcgtcg
     3841 cggcagcagt agtcgaggta ccagcgcagc tggcggtcat gaaacccctg ctggttcagc
     3901 catgcttcaa acgtaatagc gtcttgcgca agctggacgg gcgaaaacga cgatttctgc
     3961 actgggatgg cgtagtgcgc ggcggcctgc gcgctggcca ccgcggcggc aaagcgccgg
     4021 tactgcgcca gcgtggcgcc gcccacgccc tgcagcggca gcagaccgtc ctgccactgg
     4081 ccgtgcagga acaggcgctc ctgcgggctg tggcacaggg cgcgttcgtc gtactgccag
     4141 cgcccggcca cgcgccggcg caggcccagc tcttccagca ggtcctgcac cgcgcgggca
     4201 tcgtcgccgg gcagcggcag gtagtgcgcg cccatcgggc aggcgatgcc gttcacctgc
     4261 atgccgcggc tgtggccacc ggccgtgtct tccagctcca gcagggcgaa gtcgtcgtgc
     4321 ccggccaggc gcagcgcgcg cgccgccgcc agcccggcga tgccgccgcc ggcgatcacg
     4381 gtgtgcacgt gccgcgtgag gtcgggcgcg cgcgtgggcc agggctggcg cagccggtgg
     4441 ccgcgcgcgt cgctgtcgtt ggcaaaaccg ccttcgatgg cctgcgccgc gtcgcgcgtg
     4501 caggccgcgc ccagcagtgc gccggcgccg gccgccagcc agttgcggcg gcgccagccg
     4561 gggctcattc ctgcacccgt ccccattcgg tttcgtaggt gtgcaccagc gcctggttgg
     4621 acaggcggtt gacctcggcc ggcacgcgcg ccatgtcggc cggaaaatcg aacagcagcg
     4681 gcagcgtgtg cggcgtcaga aaacgcaggc cgtcgggcag ggtgccgggc aggcgccagg
     4741 gcctgcggcc ggcgacgatg aacccccact ctccgaagct gggcacatgg gcgtggtagg
     4801 gggtggcgct caggcccgcc gcttcgaccg tctgcaccac ggtccagaag ctgcgccgcg
     4861 ccaccagcgg cgacgtggtc tggatcaccg cgtagccgct ggcggccagg cgctgctcca
     4921 gcagggcgta gaagctgagc gtgtagagct tgccgatgct gaagttggtg gggtcgggaa
     4981 agtccaccac gatgacgtca tagaaggctt cgcccccacg cgccgccagc gggtcggcgg
     5041 cggcgggttc ctgggcggtg ggcgcgacgc cctgctgcag ccactgaaag gcgtcggtgt
     5101 tgacgatctg cacccggggg tcgcgcagcg ccccgtggtt gagctgcgcc agctgcggcg
     5161 tgtcgcgaaa cagctgcgtc atggccgggt ccagctcgac cagggtgacc gattcgaccg
     5221 aggggtactt gagcacctcg cgcaccgcca tgccgtcgcc gccacccagc acggcgaccc
     5281 ggcgcggtgc gccatgcgcc gccatcgccg ggtgcaccag ggcctcgtgg tagcggtact
     5341 cgtcgcgctg ggcgaactgc aggttgccgt tcagaaacag ccgcaggccg ccgtgcccgc
     5401 cggtcaccac gatgcgctgg tagggcgtgg attcggcaaa ggtgatgggg gcctggtaga
     5461 agtgctcttc ggccaggctg gtgatgcgcc cggcacccgc catgccggcc gccagcagcg
     5521 ccaccaccac cgcactggcc acggcaaatg cgcgccggcg cggcaggcca tggcgaaaca
     5581 gccacagcgc ccagatcgcc accgccgcgt tcatcaaccc gaacagaaag ccggtgcgga
     5641 tcagccccag ccgcggcacc agcaccagcg gaaaggccag cgacaccgcc agcgcgccga
     5701 ggtagtcgaa ggtcagcacc tggctgacca ggtcccggat cgtcacgtcg cgcttgagga
     5761 tgcgcatgac cagcgggatt tccagcccca ccagcgtgcc caccaccagc accatgccgt
     5821 acagcagcgg ccgaaacgcc cccggcacgg tggcgttggc gacgaacagc agcgccggca
     5881 gcgcgccgcc gaacagcgcg atcagcagct cgatgcgcag aaagtgcgcc ggcaactggc
     5941 gctcgaaata gcgcgacagc cacgagccca cgcccatggc gaacaggtag gtgccgatga
     6001 cggtggaaaa ctgcagcacc gaatcgccca gcagatagct ggccagcgcg cccgccgcca
     6061 gttcgtacag caggccgcag gcggcgatga cgaacacgct ggccagcaac gccacggcca
     6121 tcggacgggg ggcggcgccc gtgggcgcga ccagggtgga cgaagatgcc gtcatgacag
     6181 gggggcgcgg ccacgcccgg cgctgcggcg cgccggcgcg tgccgcggcg tcaatggatg
     6241 gccgccgcca cgatgatgct gatgcccagg cacatggcgg ccaccaccag ggccagcgcg
     6301 cggttgtgct tttcgacgat ttcctgccac aggttgacgg gcgtgagctt gtcgatgatg
     6361 acgaagcaca gccagaagat cgccaccccg atcagggcgt agacgagcga gccgatcagg
     6421 gcggcgggtt tgagccattc agcgtccatg gtgatgacct ccgtgggtgg ataacctcat
     6481 ttgtggctgc cgccgcttga gtagccgcca aaggcgccgc cggtgccgcg cgccaggtag
     6541 tcgtcgagct caagcgagca gctgtcgagc accgtcgaga tgacgatgat cacgacgatg
     6601 gccatgatga tcttctgcgt catgccctcc gcggccagct cggcgggccc gatcacggcg
     6661 cgctcgaaca gcgccttgcg ttggctcatg ccaaaggcct cggccaccgc cgcaccgtcg
     6721 atgcggctgc cgaccgaggc cgtgacctcg ccgttgctgc gctcggtgga cagcagcttg
     6781 ttgccgttgg cgtagtcgtg gttgtcggtc ttctggccgc gctcgaccgg ccagtagaac
     6841 tcgcccagca cctcggtggt ggtggcctcg taggccgact gcagggtgta ggtgctgccc
     6901 tggtagcgcg cgcgccgctt gccctggtcg atgcgcggcg cgccggtggc ggtgaacacc
     6961 aggctccagc cgtcttcggt gtcgaccaga aaggcaaagc cgcgctcgcg gtggtagagc
     7021 aggtactcct tccactcgaa cttctcgtcg tcgcccggct cgcgcccgct gcgccgctgc
     7081 cagcccacca cctgccagct cgtgccctgc agctggccgc ggctgcccag ggggatgagc
     7141 ggcggcacgc gcactttctg cttggccgcc agcagctcgc cgccgacgcc ggccgtcaga
     7201 tcgatcaggc tctcgcactg cgcgcaggtg cagcgcttgg tgttgtcgag ttgcacatgc
     7261 accggcgcgc cgcagttggg gcaggcgaag gagcgcccgc cctggtcctc gcgcaccgat
     7321 gcgtccttca ggcccttgag ctgcaagtgc gccagttcca ccgcgcggcc gcgctcgacc
     7381 tgcggcggcg cggcgccgta gtcgatggac agcacctggc cgtcttcact gcgcagctcc
     7441 agcagcttga agggctggcc cagcgcgggc agcttgggca gctcgccctc ggcggcgatc
     7501 agctgggcgc tgccgctgta cgccaccacc cagtcggtga cgcccaggcg caggctgctg
     7561 ccggcggccg gcagcggctg ggccggcggc ggctcggtgc cctcgtcggg ccgggtgaag
     7621 acgtagctgc cgttgtcttc gctcagcgtg ccggtgcggc catcgtccag cgcggcgatc
     7681 cactcggtcc aggtgccgga atcgctcttg aattgcaggc gcccgatcag ggtgaagggc
     7741 agcttgttgc cgtccagcgt gacgcgcccg ctggccatca gctgcagcgg gctgtggtcg
     7801 tcgaacagct cggccatctg gccgatgcgc tgaagctgct caccgctgcg caccaccgtg
     7861 ctgcggcaga agccgcacac cgcaaagggc gcggtggcgc tctggaactc caccggcgcg
     7921 ccgcaggacg ggcaggcggc gcggtagtga cgctgcggtg cgtcggtggc catgttatgg
     7981 gtgttttatg gctccggcgc gcatccaaca agcgccagca gctatcaaaa aagtagcgct
     8041 cagaccagtt tcttgagcag ctcggccttc ttggcgttga actcgtcttc ggtcaggatg
     8101 cccttggcct tgagctcgcc cagcttctcc agcgtggcca tcacgtcttc gggcctgacg
     8161 cccgcggccg gcgtggcgcc gccctgcagc gccgactgca tgttctgcgc caggatctgc
     8221 cccatggcca cgcccgcgcc catgcccagg ccggcgccgg ccagcccgcc cgcgtcgccg
     8281 ccggcgccca cgccctcggc catcttggga atggcctgcg cggtttggta ctgcatgaac
     8341 ttgcccatgt cgccgccgac catgcccatg ccgatgcgct ggtccagcac tttctgcagc
     8401 tcttcgggca gcgaaatgct ctgcaccgtc atgccttcca gcttgaggcc gtaggcttcc
     8461 atggccggtg ccagcgcatt cgacagcgcc tgggcaaagg cctgctggtt ggccgccagg
     8521 tccagaaacg ggatgccgct ttgcgcaatg ccggtggcga tgtgctgcgt gatcagcccg
     8581 cgcagctggc cgtccaggtc atcgacggcg tagacatcgc gcgtgccgga gatttcggtg
     8641 tggaacttct tggggtcggc cacccgatag gcgtagttgc cgaaggcgcg caggcgcacc
     8701 gcgccgaact ccttgtcgcg gatgctgatc ggctggggcg tgccccactt ctggtcgacc
     8761 tgctggcgcg tgctgaagaa gtaga
//
LOCUS       DAOQTZ010000101         8756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_8910_length_8756_cov_14.134697, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000101 DAOQTZ010000000
VERSION     DAOQTZ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8910_length_8756_cov_14.134697"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..1113)
                     /locus_tag="PKN51_11005"
     CDS             complement(295..1113)
                     /locus_tag="PKN51_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNT85670.1"
                     /translation="MTPRSTLAALLCAALLAAGCASTTSGSVATGGRGRSQLLLVSSE
                     QVMQQSLQYYDEQNREARAKGQLATSGPEWGRVNAIMQRLVPQVAAFRPDAARWPWQL
                     VLIDEDSVNAHVMAGGKITFYTGLIRKLRVSDDEIAAVMGHEMAHALREHTREKMSQA
                     AAGDLAIAVGGALLGVGQGAVQLASLGKQLALDLPFSRHMESEADLYGLELAARAGYD
                     PRAAVSLWQKMAQAGGGNGPGFLSTHPSAGDRMGQLQAAMPRVLPLYEAARARH"
     gene            complement(1313..1480)
                     /locus_tag="PKN51_11010"
     CDS             complement(1313..1480)
                     /locus_tag="PKN51_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85671.1"
                     /translation="MQGTTLRRWSFWLLLTGFVAWDFASSAPVNLRLEPPIITAGSGS
                     PASGGHCAMPK"
     gene            complement(1498..2634)
                     /locus_tag="PKN51_11015"
     CDS             complement(1498..2634)
                     /locus_tag="PKN51_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNT85672.1"
                     /translation="MNTLAPALDAAAPTRAARWSQRLPLAIAVLALFGWLLWSVSARQ
                     ALLLLVGVGLGAVLAGARFGFTTGWRMLVEQRDPAGVYGQLLLLALLSALSMPLLARF
                     PETTAALGPLSVSLLVGAFVFGLCMQIADGCGSGTLYKAGLGVPLNMAILPLFALGSF
                     AGSAHLNGWLALGALPPVGLVSAFGAGGALAATGVALALIALGVGRWSGQRLDLRRLP
                     RRWLWGALGLALLAALNLLIAGQPWGVVYGFGLWAAKLATALGAFEPSANAFWSEPGH
                     AERLQQTLLLDVTSITNIGILAGALWVSARRPESPRQLTGAQWAIGLLAGFIMGYSSR
                     LAFGCNVGAMVSGISTGSLHGWLWVPLAFAGTLIGLRVRRRFGF"
     gene            complement(2631..3587)
                     /locus_tag="PKN51_11020"
     CDS             complement(2631..3587)
                     /locus_tag="PKN51_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNT85673.1"
                     /translation="MRRPFRFLLLALAWATASLAAQPLLTPAQVKALATDPVVRLIDV
                     REASAYAMQHLPGALSAPYGRWRTSDANLGLPPTPAELTQLVQELGLTPDTHAILVYT
                     GIDATDFGGAARAYWTLKSLGMRRLSILNGGLTAWKAEGLPVSDQPARAPRSQWQPRL
                     QAQWLATRDDVRASLAQPEVLRVDARPAAYFQGRLAHDNARARGTLPGAVHLDSEVFF
                     ELGSAALLDKATLESEAQALNATPGQAIITFCNAGHWSATDWFVLSEVLGQPNVRMYP
                     GSMVDWTRASAPLPMIHEPGRWQQLRYALLTWAHRNLGTPAP"
     gene            3850..4725
                     /locus_tag="PKN51_11025"
     CDS             3850..4725
                     /locus_tag="PKN51_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT85674.1"
                     /translation="MQFVQLVISGVALGCIYGLIALGFVLIYKATETVNFAQGELMML
                     GAFCGLAGSTLLGFPFWVSVLGTLAAMALFGVLTERLVIRPILGQPAFSIVMLTIGIG
                     YVARGAITMIPAIGTETHTLPVPYKDQVWRLGELVLSAEQVVVIGVTAVLCALLYAMF
                     RFTRLGIAMQAASQNQLAAYYMGIPVKRLNGLVWGLAALVAAIAGLLLAPITFVYANM
                     GFIGLKAFPAAVVGGFGSLPGAIVGGLIIGVVESLAGFYLPEGFKDTAAYIVVLVMLM
                     VKPNGLFGEKLRKKV"
     gene            4727..5803
                     /locus_tag="PKN51_11030"
     CDS             4727..5803
                     /locus_tag="PKN51_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT85675.1"
                     /translation="MRFIFKTSYAQDIRLAKHGGHVFWYGLLAVGLLLAPRLLDEYWL
                     AQLTFVLIYAIAGLGLMLLAGFTGQFSLGHAAFLGVGAYTQAILTNAGWPFPLAMLCA
                     AVLAAAVGVVVGLPALRVKGIYLGIATLAFGFIVEEVLARWESLTGGNAGLMAGAPAM
                     FGWTIDSSAGFYAICLITAVLATLGTLNLLRTPTGRAFIAIRDSEISAQSMGIHLARY
                     KTLAFALSAALAGLAGALYAHKLQFLSPDQFNILQSIDLLLLVVIGGLGSVHGAFLGA
                     IFLIAMPQLIALGKDYLPEAIGQAPGLQAVVYGAVLIAFVLFEPMGLYGRWLKIRTWL
                     QLFPFYRQGMFKRQKSFQKSDRLK"
     gene            5800..6612
                     /locus_tag="PKN51_11035"
     CDS             5800..6612
                     /locus_tag="PKN51_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85676.1"
                     /translation="MTPDTLLSARNLSVRFGGVLAVNDVSFDVRKGEVFTLIGPNGAG
                     KTTVFNLISRIYQPTGGEIDYAGPHGQVRLTQQAPHQVASLGIARTFQNIELFEHASV
                     LHNLLVGQHTHRRTGFWSDLLFTPAARTAELATRERVERVIDFLDLQHYREALVAGLP
                     YGVRKVVEMARALCAEPRLLLLDEPSSGLNVEETDDMVFWIQDIVHELGITVLMVEHD
                     MSLVSRVSDRVLAMNQGQVLAMGTPAEVQADARVAEAYLGSIDDVASLRRQA"
     gene            6609..7382
                     /locus_tag="PKN51_11040"
     CDS             6609..7382
                     /locus_tag="PKN51_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85677.1"
                     /translation="MSEPILQLLNVESSYGPIKAIRGVSLRVEPGQIATVLGSNGAGK
                     STILKTISGIIDPGKGTIHFRGEDITARDPALIVQKGLSHVPEGREVFPLLSVHDNLL
                     MGAYTRKDRDGVARDMEQVYAYFPILRERAAQDAGLLSGGQQQMLAISRALMAAPEMI
                     LLDEPSLGLSPRLTKEIFEIVVRINRERGTTILLVEQNANMALNAADYGYVLENGRIV
                     MEGSCDVLREKEDIKEFYLGMKDAGVRGERRWKKKKTWR"
     gene            7388..>8756
                     /locus_tag="PKN51_11045"
     CDS             7388..>8756
                     /locus_tag="PKN51_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT85678.1"
                     /translation="MSNLWDLSHIQPREDVVIAGDTLAAMFWNSVAERGPQVWLREKD
                     LGIWKSWTWTQVGEAVAEIAGGLMSLGFARGDTASILSNTVVEWVLTDLAVLSCGGVA
                     NGIYPTDAASQVHFLSEDSRTRVLFVEDDEQLDKALEVRDRLPLLTRIVVFDMTGLRD
                     LRDEGVISLDALRELGRAYNQRHPDELQQRVAAVRPEDLAILVYTSGTTGKPKGAMHT
                     HAGLVYTARGFNTLIARTEDDECMCFLPLCHIAERMGGEYFSLYTGAKLNFVENPDTV
                     PENVREIAPTAFTAVPRVWEKFYSGVTIALKESTRLQQATYAWAIGVGRQITERVLAG
                     QPVPGSLKLRFRLARWLALDNVRKLIGIHRARLLVTGAAPISPELVKWYLSLGVPMLE
                     VWGMTETCGASTGVLASRIKPGSIGPAATYNQVRLDPATGEIQVKGPNVFAGYLNLPE
                     KTAETF"
BASE COUNT         1361 a         2950 c         3074 g         1371 t
ORIGIN      
        1 tcttgcgact tcgatggcct tcacctcttg ggtgagcacg ctgcgcaccc gcccgtaccg
       61 cgctcggcgt gccatgcggc gttgctcctg cttgcgggca ccagcccgcg gcggcgtcgc
      121 gtcttgcctg gcacgccgat cacggcacgg gcgggcgcgt ccaactgccg tttctaggct
      181 gcgcctgcac gcggccgcgg accgtctgcc atcgtcgggc ggtcggcatc ccgtcagggc
      241 cgcgcggcct ggctgaccgg cacctgccgg gggatgggcg ccgtcgctgg agactcagtg
      301 ccgggcgcgc gccgcttcgt acagcggcag cacgcgcggc atggcggcct gcaactggcc
      361 catgcgatcg cccgcgctgg ggtgggtgga cagaaagccc ggcccgttgc cgccgccggc
      421 ctgcgccatc ttctgccaca ggctgacggc ggcgcgcggg tcgtagccgg cgcgcgcggc
      481 cagctccagc ccgtacaggt cggcctcgct ttccatgtgg cgcgaaaacg gcaggtccag
      541 cgccagctgc ttgcccaggc tggccagctg caccgcgccc tggcccacgc ccagcagcgc
      601 gccgccgacg gcgatggcca gatcgcccgc cgccgcctgc gacatcttct cgcgcgtgtg
      661 ctcgcgcagc gcatgcgcca tctcgtggcc catgacggcg gcgatctcgt cgtcgctcac
      721 gcgcagcttg cggatcaggc cggtgtagaa cgtgatcttg ccgcccgcca tgacgtgggc
      781 gttgacgctg tcctcgtcga tcagcaccag ctgccagggc cagcgcgcgg cgtcggggcg
      841 aaacgccgcc acctgcggca ccaggcgctg catgatggcg ttgacgcggc cccattccgg
      901 cccgctggtc gccagctggc ctttcgcgcg cgcctcgcgg ttctgctcgt cgtagtactg
      961 cagcgactgc tgcatcacct gctcggacga caccagcagc aactgcgagc gaccgcggcc
     1021 tccggtggcg acgctgccgc tggtggtgct ggcgcagccg gcggccagca gggcggcaca
     1081 gagcaaggcg gcaagggtgg aacgcggtgt catgcagcgg tcggtcggtg gtgtggcgaa
     1141 gcgcgacggc gaccattgtg ccgccaccac cgttctctgt gcagggcgcc gccatggcgc
     1201 ggcgggcagg ttggcggatt ctgtgacaaa tggaggtctg gcgcaggcgg ggtgtgcgcc
     1261 aacagctaca aatacgatag cgtatgggac ggggcgagtg ggcgccctga cctcacttgg
     1321 gcatggcgca gtgcccgccg ctggccgggc tgccggagcc ggcggtgatg atgggcggct
     1381 ccagccgcag gttgaccggc gccgaactgg cgaagtccca ggccacgaag ccggtcagca
     1441 gcagccagaa gctccagcgg cgcagggtgg tgccttgcat gtcgatgtcc cccgttctca
     1501 gaaaccgaag cggcgccgca cgcgcaagcc gatcagggtg ccggcaaagg ccagcggcac
     1561 ccacagccag ccgtgcaggc tgccggtgct gatgccgctg accatggcgc cgacgttgca
     1621 gccaaaggcc aggcgcgagc tgtagcccat gatgaatccc gccagcaggc cgatcgccca
     1681 ctgcgcgccg gtcagctgcc ggggcgattc ggggcgccgc gccgacaccc acagcgcgcc
     1741 ggccaggatg ccgatgttgg tgatgctggt cacgtccagc agcagggtct gctgcagccg
     1801 ctcggcatgg cccggctcgc tccagaaggc gttggccgac ggctcgaacg cacccagcgc
     1861 cgtggccagc ttggccgccc acaggccgaa gccgtacacc acgccccagg gctggccggc
     1921 gatcagcagg ttcagcgccg ccagcagcgc cagccccagc gcgccccaca gccagcggcg
     1981 cggcagacgg cgcaggtcca ggcgctggcc gctccagcgg cccacgccca gcgcgatcag
     2041 cgccagcgcc accccggtgg cggccagcgc gccgccggcg ccgaaggcgc tcaccagacc
     2101 gaccggcggc agcgcgccca acgccagcca gccgttcagg tgggccgaac cggcaaagct
     2161 gccgagcgcg aacagcggca ggatggccat gttcagcggc acccccagcc cggccttgta
     2221 cagcgtgccc gagccgcagc cgtcggcgat ctgcatgcac aggccgaaca cgaaggcacc
     2281 caccagcagg ctgaccgaca gcggccccag cgccgccgtg gtttccggaa agcgcgccag
     2341 cagcggcatc gacagcgccg acagcagcgc cagcagcagc aactgcccgt atacgccggc
     2401 cggatcgcgc tgttcgacca gcatgcgcca gccggtggtg aagccaaagc gcgcgcccgc
     2461 cagcaccgcc cccaggccga cgcccaccag cagcagcagc gcctggcgcg ccgacaccga
     2521 ccacaacagc cagccgaaca gcgccagcac ggcgatcgcc agcggcaggc gttgcgacca
     2581 gcgcgcggcg cgggtgggcg ccgcggcgtc cagggcaggg gcaagcgtat tcatggggca
     2641 ggggtcccga ggttgcggtg cgcccaggtc agcagcgcgt agcgcagttg ctgccagcgg
     2701 ccgggctcat ggatcatggg caatggggcg gatgcgcgcg tccagtccac catcgagccg
     2761 gggtacatgc gcacgttggg ctgccccagc acttcggaca gcacgaacca gtcggtggcc
     2821 gaccaatgac cggcgttgca gaaggtgatg atggcctgac ccggcgtggc attcagggcc
     2881 tgggcctcgc tctccagcgt cgccttgtcc agcagcgcgg cgctgcccag ctcgaagaac
     2941 acctcgctgt ccaggtgcac ggcgcccggc agcgtgccgc gtgcgcgggc gttgtcatgc
     3001 gccaggcggc cctggaaata cgccgccggg cgcgcatcca cacgcagcac ctcaggctgc
     3061 gccaggctgg cgcgcacgtc gtcgcgcgtg gccagccact gcgcctgcag gcgcggctgc
     3121 cactggctgc gcggtgcgcg ggcgggctgg tcgctgacgg gcaggccttc ggccttccag
     3181 gccgtcagac caccgttgag gatggacagg cggcgcatgc cgagcgactt cagcgtccag
     3241 taggcgcgcg cggcgccacc gaagtcggtg gcgtcgatgc cggtgtagac caggatggcg
     3301 tgggtgtccg gcgtcaggcc cagctcctgc accagctggg tgagttccgc tggcgtgggc
     3361 ggcaggccca ggttggcgtc gctggtgcgc cagcgcccgt agggggccga cagcgcgccg
     3421 ggcaggtgct gcatggcgta ggccgaggct tcgcgcacat cgatcagccg caccaccggg
     3481 tcggtcgcca gcgccttgac ctgtgccggc gtcagcagcg gctgggcagc cagcgaggcg
     3541 gtggcccagg ccagggcaag caacaggaat cggaacggac ggcgcatggc gggcacggag
     3601 cagggcgatg gagcaaaccc cgcattgtcg cgccggtcgc ggtggcgacc aaataccgag
     3661 tcgtcagaag catatggggc accgtcttgc cccacatcgg tgcacgccgg tgtcacggat
     3721 gggacacgga gggtgcatgg cggcgcgacg gtcgcgccac gctaagggtt tgtcagggat
     3781 gtggctgcag atcaagcccg ttagagtctg gcgcactttg atgcggcaca aggatagggt
     3841 ggaccgagcg tgcaatttgt gcaactggtg atcagcgggg tggccctggg ctgcatctat
     3901 ggtctgatcg ccctcggctt cgtgctgatc tacaaggcca ccgagaccgt caacttcgcc
     3961 cagggcgagc tgatgatgct gggcgccttc tgcggcctgg ccggcagcac cctgctgggc
     4021 tttccgttct gggtgtcggt gctgggcacg ctggcggcga tggcgctgtt cggcgtgctg
     4081 accgagcggc tggtgattcg ccccatcctc ggtcagccgg cgttctcgat cgtcatgctg
     4141 accatcggca tcggctatgt ggcgcgtggc gccatcacca tgatcccggc catcggcacc
     4201 gaaacccaca ccctgcccgt gccctacaag gaccaggtct ggcggctggg cgagctggtg
     4261 ctgagcgccg agcaggtggt ggtgatcggt gtcacggcgg tgctgtgcgc gctgctgtac
     4321 gcgatgttcc gtttcacgcg cctgggcatc gccatgcagg ccgcctcgca gaaccagctg
     4381 gccgcctact acatgggcat cccggtcaag cgcctcaacg gtctggtgtg ggggctggcg
     4441 gcgctggtgg cggccattgc cggtctgctg ctggcgccga tcacgttcgt ctacgccaac
     4501 atgggcttca tcggcctgaa ggcgttcccg gccgccgtgg tggggggctt cggcagcctg
     4561 ccgggggcca tcgtcggtgg tctgatcatc ggtgtggtgg agtcgctggc gggcttttac
     4621 ctgcccgagg gtttcaagga caccgccgcc tacatcgtgg tgctggtcat gctgatggtc
     4681 aagcccaacg gtctgttcgg cgagaagctg cgcaaaaagg tctgacatgc gcttcatctt
     4741 caagaccagc tacgcgcagg acatccgtct ggccaagcat ggcgggcatg tgttctggta
     4801 tggcctgctg gcggtcggcc tgctgctggc gcccaggctg ctggacgaat actggctggc
     4861 gcagctgacc ttcgtgctga tctacgccat cgccggtctg ggcctgatgc tgctggccgg
     4921 cttcaccggg cagttctcgc tggggcacgc ggcgttcctg ggcgtcggcg cctacaccca
     4981 ggccatcctg accaacgccg gctggccgtt tccgctggcc atgctgtgcg ccgccgtgct
     5041 ggcggccgcc gtcggggtgg tggtgggcct gccggcgctg cgcgtcaagg gcatttacct
     5101 gggcatcgcc acgctggcct tcggtttcat cgtcgaggag gtgctggcgc gctgggaaag
     5161 cctgaccgga ggcaacgccg gcctgatggc gggtgcgccg gcgatgttcg gctggaccat
     5221 cgactccagc gccggcttct acgccatctg cctgatcacc gccgtgctgg ccaccctggg
     5281 cacgctcaac ctgctgcgca cgcccaccgg gcgcgccttc atcgccattc gcgactcgga
     5341 aatctcggcg cagagcatgg gcattcacct ggcgcgctac aagacgctgg ccttcgccct
     5401 gtcggccgcg ctggcgggcc tggcgggggc gctgtacgcc cacaagctgc aattcctgtc
     5461 gcccgatcag ttcaacatcc tgcagtcgat cgacctgctg ctgctggtgg tgatcggcgg
     5521 tctgggctcg gtgcacgggg cgtttctggg cgccatcttc ctgatcgcca tgccgcagct
     5581 cattgcgctc ggcaaggact acctgcccga agccatcggt caggcgcccg gcctgcaggc
     5641 ggtggtctat ggcgcggtgc tgatcgcctt cgtgctgttc gagccgatgg ggctgtacgg
     5701 tcgctggctc aagatccgaa cctggctgca gctgtttccc ttctaccgcc agggcatgtt
     5761 caagcggcag aaatcgttcc agaaatcgga tcgcctcaaa tgacgcccga taccctgctt
     5821 tcggcccgca acctgagcgt gcgcttcggt ggcgtgctgg ccgtcaatga cgtcagcttc
     5881 gacgtgcgca agggcgaagt gttcaccctg atcggcccca acggcgccgg caagaccacg
     5941 gtgttcaacc tgatcagccg catctaccag cccaccggcg gcgagatcga ctatgccggg
     6001 ccgcacggtc aggtcaggct gacgcagcag gcgccgcacc aggtggcgtc gctgggcatc
     6061 gcgcgcacct ttcagaacat cgaactgttc gagcacgcca gcgtgctgca caacctgttg
     6121 gtggggcaac acacgcaccg gcgcaccggc ttctggagcg atctgctgtt cacaccggcg
     6181 gcgcgcacag ccgagctggc cacccgtgag cgcgtcgaac gcgtgatcga cttcctcgat
     6241 ctgcagcatt accgcgaagc gctggtggcg ggcctgccct atggcgtgcg caaggtggtt
     6301 gagatggcgc gcgcgctgtg cgccgaaccg cgcctgctgt tgctggacga accttcgtcc
     6361 ggcctgaacg tcgaggaaac cgacgacatg gtgttctgga tccaggacat cgtgcatgag
     6421 ctgggcatca cggtgctgat ggtggagcac gacatgagcc tggtctcgcg tgtgtccgac
     6481 cgcgtgctgg ccatgaacca gggccaggtg ctggccatgg gcacgcccgc cgaggtgcag
     6541 gccgacgcca gggtggccga ggcctacctg ggctcgatcg acgacgtcgc ctcgctgcgg
     6601 aggcaggcat gagcgagccg atcctgcagt tgctcaacgt cgagagctcg tacggcccga
     6661 tcaaggcgat ccggggcgtc agtctgcgcg tggagccggg ccagatcgcc accgtgctgg
     6721 gcagcaacgg cgccggcaag agcaccatcc ttaaaaccat ctccggcatc atcgacccgg
     6781 gcaagggcac gatccatttc aggggcgagg acatcaccgc gcgcgacccg gcgctgatcg
     6841 tgcagaaggg tctgtcgcac gtgcccgagg ggcgtgaggt gtttcctctg ctgtcggtgc
     6901 acgacaacct gctgatgggc gcctacaccc gcaaggatcg cgacggcgtg gcgcgcgaca
     6961 tggagcaggt ttatgcctac tttccaatcc tgcgcgagcg cgctgcccag gatgccggcc
     7021 tgctgtcggg tggccagcag cagatgctgg ccatcagccg cgcgctgatg gcggcgcccg
     7081 agatgatcct gctggacgag cccagcctgg gcctgtcgcc cagactgacc aaggaaatct
     7141 tcgagatcgt ggtgcgcatc aaccgcgaac gcggcaccac catcctgctg gtcgagcaga
     7201 acgccaacat ggcgctcaat gccgccgatt acggctacgt gctggagaac ggtcggatcg
     7261 tgatggaggg cagctgcgac gtgctgcgcg agaaggagga catcaaggag ttctatctcg
     7321 gcatgaagga cgccggcgtg cgcggcgagc ggcgctggaa gaagaaaaag acctggaggt
     7381 aagcccgatg tccaacctgt gggatctctc gcacatccag ccgcgcgaag acgtggtgat
     7441 cgccggcgac accctggccg ccatgttctg gaacagcgtg gccgagcgcg gcccgcaggt
     7501 gtggctgcgc gagaaagacc tgggcatctg gaagagctgg acctggaccc aggtgggcga
     7561 ggcggtggcc gagatcgccg gcggtctgat gagcctgggt tttgcccgcg gcgacaccgc
     7621 ctcgatcctg tccaacaccg tggtggagtg ggtgctgacc gatctggcag tgctgtcctg
     7681 cggcggggtg gccaacggca tctaccccac cgacgcggcc agccaggtgc attttctcag
     7741 cgaggattca cgcacccggg tgctgttcgt cgaggatgac gagcagctcg acaaggcgct
     7801 ggaagtgcgc gaccggctgc cgctgctgac caggatcgtg gtgtttgaca tgacgggctt
     7861 gcgcgatctg cgcgacgagg gcgtgatcag cctggatgcg ctgcgcgaac tgggccgcgc
     7921 ctacaaccag cgccacccgg acgagctgca gcagcgcgtg gccgcggtgc gccccgaaga
     7981 cctggcgatt ctggtctaca cctcgggcac caccggcaaa ccgaagggcg ccatgcacac
     8041 ccatgcgggg ctggtctata cagcgcgcgg cttcaacacg ctgatcgcgc gcaccgaaga
     8101 cgatgagtgc atgtgctttc tgccgctgtg ccacatcgcc gagcgcatgg gtggcgagta
     8161 tttttcgctg tacaccggcg ccaagctcaa ctttgtcgag aaccccgaca ccgtgcccga
     8221 gaacgtgcgc gagatcgcgc ccacggcctt caccgcggtg ccgcgcgtgt gggagaagtt
     8281 ttattccggc gtcaccatcg cactgaagga gagcacgcga ctgcagcagg cgacctacgc
     8341 ctgggccatc ggcgtggggc gccagatcac cgagcgggtg ctggccggcc agccggtgcc
     8401 gggcagcctg aagctgcgct tcaggctggc gcgctggctg gcgttggaca acgtgcgcaa
     8461 gctgatcggc attcaccgcg cacgcctgct ggtgacgggc gcggcgccga tctcgcccga
     8521 actggtcaaa tggtatctgt cgctgggcgt gccgatgctg gaggtgtggg gtatgaccga
     8581 gacctgcggc gcctccaccg gcgtgctggc ctcgcgcatc aaaccgggct ccattggccc
     8641 ggccgccacc tacaaccagg tgcggctgga cccggccacc ggggaaatcc aggtcaaggg
     8701 ccccaacgtg ttcgccggct acctgaacct gcctgaaaag acggccgaaa cgttta
//
LOCUS       DAOQTZ010000102         8722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_8955_length_8722_cov_10.130841, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000102 DAOQTZ010000000
VERSION     DAOQTZ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8955_length_8722_cov_10.130841"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1979
                     /locus_tag="PKN51_11050"
     CDS             102..1979
                     /locus_tag="PKN51_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HNT85679.1"
                     /translation="MASQLPPEDGEDAADAQRGFIATLPDAEVRRADGGSVWSQRAYG
                     FLDDEQSPDTVHPGLWRQARLNRVHGLFQVTARIFQVRGLDIANITFIEGDEGLIAVD
                     ALTCAETAAAALALYRKHRDPLSRRPLHTVIYTHSHADHFGGVHGLVDEADVRAGRVQ
                     VIAPAGFLHHAVAENVLAGVAMARRSQFQFGQTLPKGPAGQVDAGLGKTLPLGRPGLI
                     APTREIAAEHEVLTIDGLAIEFQLTPETEAPAEMHLFIASEGALNLAENATHTLHNLC
                     PLRGAQVRDALAWSKYLNQALHRYGPRTEVVLAQHHWPTWGRDRACRLLAEQRDLYRL
                     LHDQTVRLMSHGLKAAEIAEGLQLPPALAARWHTRGFYGTVSHNVKAIYQHYLSWYDA
                     NPANLHALPPVEAARKTVAYMGGADALLARARDDFARGEFRWVAEVMKQLVYAQPDLA
                     PARELAAAALEQMGFQAESATWRNAFLLGAREYRDGPPPPPPAGAGMAMLGALDNELL
                     FDALAVRVHAGRAEGLAFTLAWHFTDRDELWLSTLSHGALSSLRIADPAVAAAADLRI
                     TTDRPTLDAILQQRLPPPQAVSEGRLQLQGQAALLPRFFGLLDRFSGSFAVVDAAPWP
                     L"
     gene            2007..4298
                     /locus_tag="PKN51_11055"
     CDS             2007..4298
                     /locus_tag="PKN51_11055"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNT85680.1"
                     /translation="MSSPDTPRTGRTIAESVPHWPARPQRPAGAPNILVILFDDVGFS
                     DFGCYGSSIRTPNIDGLAAQGLRYTGFHTTAMCSTTRAALLTGRNHHAVGMGCLANFD
                     SGFDGYRGKISPAAGTLAEMLRPQGWRNYMVGKWHVTPLTQTGPAGPYDGWPLGRGFD
                     RYYGFMDAETDQYAPELVRDNTHVDPPATPDQGYHLTADLVDEAIRMLADHQADAPDA
                     PWLLWLALGACHAPHQAPQDLIRSYDSQFAHGWDVEREQRLARQKALGLVPADTELPP
                     RNDGVPAWDTLGEAERRVFTRLQSAFAGMLDHADQHIGRLLAFLRDARLDDDTLVLVL
                     SDNGASQEGGPLGFVNAMGPYNLKPEDMAEKLRRIDDIGGIDTHSNFPQGWAMASNTP
                     LKRYKQNTHGGGIRDPLVMAWPKGISARGELRHPFCHASDLLPTLLELCRVQPPAQIN
                     GVAQLPISGQSFAASLADPAAPARATPQYFEMFGHRGIWHQGWKAVAWHAPGTPFDAD
                     RWELYHLAEDFAENHNRADSEPERLKALQDLWWREAEANQVLPLDERFGARFAENARR
                     SQGARSQFVFHAGMGHLPTDVAPDVRSRSYRIEAELLIPEGGAEGVLIAHGDATSGYS
                     LYLQGGHLVHDLNIGGLHQLLRSDRAVPPGRRRLAFSMTLGPMVLTPPIQGLGRVEVP
                     SSRRGSLFIDGEPVGETLFKVGFTSLISWSGLDIGRDRASPVSHYAAPFEFTGRLFHV
                     TLTLAPQQALAGAAIGQAEMARQ"
     gene            complement(4336..4860)
                     /locus_tag="PKN51_11060"
     CDS             complement(4336..4860)
                     /locus_tag="PKN51_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNT85681.1"
                     /translation="MKRATLLALAALLPLAAQAELSQVSAQGFVASFRDEVKASPDAV
                     WQAIVQLPRWWNGSHTYSGKADNLSLDLRAGGCWCERWDGGAVQHAQVVLVMPGRLLR
                     LHGGLGPLQDLPVQGVLNLGIGQQDGKTVLRLHYRVGGPADAALDKLAGPVNGVLAEQ
                     FQRLKSLVETGSPG"
     gene            complement(4857..5261)
                     /locus_tag="PKN51_11065"
     CDS             complement(4857..5261)
                     /locus_tag="PKN51_11065"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNT85682.1"
                     /translation="MDKLQFSYAAVLAAAVVHFAIGGLWYSPLAFSRAWQREAGLSDE
                     QLRQAPMARIFGLAFLAALVMAFNLAAFIGAKASLGFAVFAGFATGLGWVAMSLGVLY
                     LFERRSFKLWLINSGYLVLAFTAMGAILGAWQ"
     gene            5260..6468
                     /locus_tag="PKN51_11070"
     CDS             5260..6468
                     /locus_tag="PKN51_11070"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNT85683.1"
                     /translation="MSFSGSFQRPAPSSGATRSEAHGGAAPGADASPPAPKADGGRLE
                     ADNALPNPPPAQAARRPGVRVDDRRVMLAGSLGFGLLIPQLSGLFGSLGPGDLRFWAG
                     QAAYLALALAVWAGNRWLLFRQREHFDWFGQPWRKLVMLVVANVLYTAPVTVLGLWLA
                     ALLMGQPLPRAALQLVVAVNVICVLFVTHAYETLFLIRERESDLMWVERLQRARAQAE
                     LAALKAQVDPHFLFNSLNTLGHLISHDSARARECCDTLAEIYRYVLAARDHDLVPLAD
                     ELAFLRQYQRLLELRFDQAVQLQLDPALQAAGARWLLPPLALQTLLENAVKHNRMAQD
                     EPLRLRLGLQGDWVVAHNRRRPRSSALPGSGRGLANLDERCRLLSGRALRVRRSEQDF
                     ELDLPLVAAP"
     gene            6465..7229
                     /locus_tag="PKN51_11075"
     CDS             6465..7229
                     /locus_tag="PKN51_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNT85684.1"
                     /translation="MKLFIAEDEAPARERLLELIGRVAPQAVLLGHADSVQATRDWLA
                     AHAPPDLLLLDIQLADGLSLELFRDRAPDCPVIFTTAYDQFALQAFQALAVDYLLKPI
                     ADAALARAFAKAGRLRAGFAADVAGWLATSGVPAVVYRQRLLGRQGGQPMHVLPVAQL
                     AYLVSVDKLAFAVMADGRRYLLDGTLADLALELDPARFFRASRQVLVAASAIAAYSPA
                     GKGRLRLDLRPRLDDELLVSQERAAEFKAWLQQAPA"
     gene            7313..7768
                     /locus_tag="PKN51_11080"
     CDS             7313..7768
                     /locus_tag="PKN51_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT85685.1"
                     /translation="MVKYERAALDPVFAALADGTRREVLDRLGGGPLSVSELAAGHAM
                     SLPGFMKHLAVLEAAGLIARAKQGRVVQCELAAGPLQDAAAWIVRYQSFWTGRLDALD
                     RYLHSQGDLACPPTPPSNPPPPNPQPSTCAATTPPRRKRSGAPGPTRRR"
     gene            7702..8160
                     /locus_tag="PKN51_11085"
     CDS             7702..8160
                     /locus_tag="PKN51_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNT85686.1"
                     /translation="MRRHYAAAPQAVWRAWTDPQALMAWFGPAGPGTATVAEMDLQVG
                     GRYRFAFSTPDGEAHEVSGVYQEVQPPFRLVFSWAWHSTPERVSRVTLDLQPADGGCE
                     LRFTHDRFFDERARANHQRGWPSFLQRLDGWLSATPSGSPSSVTTPRTCE"
     gene            8157..8507
                     /locus_tag="PKN51_11090"
     CDS             8157..8507
                     /locus_tag="PKN51_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNT85687.1"
                     /translation="MSTSHGSCHCGRIRFEVDGAPAGVMACNCSICQRKGALMWFVPR
                     EQLRLLTPEADSSVYRFNKHVIQHRFCPVCGIHPYGEGTDPKGQTMAAVNVRCLEDVD
                     LAALPVTAFDGRAL"
     gene            complement(8531..>8722)
                     /locus_tag="PKN51_11095"
     CDS             complement(8531..>8722)
                     /locus_tag="PKN51_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNT85688.1"
                     /translation="CLIFFAKGKELTAKESMPDDQRALSDRAIDSHVKNLRRKLERLL
                     PGQETIRSIYGVGYRFDGL"
BASE COUNT         1279 a         3166 c         3015 g         1262 t
ORIGIN      
        1 cgtgcgcatt ggtgaggatg acgccgtcgg ggctgacgat gaagcccgag ccctggccac
       61 gcataggcat ctcgcgcggc atctgccagc cttggcgcct ggtggccagc cagctgccgc
      121 ccgaagacgg cgaagacgcg gccgacgcgc agcgcggttt catcgccacg ctgcccgacg
      181 ccgaggtgcg ccgcgccgac ggcggcagcg tctggagcca gcgcgcctat ggttttctcg
      241 acgacgaaca atcgcccgac accgtgcacc ccggcctgtg gcggcaggcg cggctgaacc
      301 gggtgcacgg cctgttccag gtgacggcgc gcatcttcca ggtgcgcggg ctggacatcg
      361 ccaacatcac gttcatcgaa ggcgatgaag gcctgatcgc ggtcgacgcg ctgacctgcg
      421 ccgaaaccgc ggcggcggcg ctggcgctgt accgcaagca ccgcgacccc ttaagccgcc
      481 ggccgctgca cacggtgatc tacacgcaca gccatgccga ccacttcggc ggtgtgcacg
      541 gcctggtcga tgaagccgac gtgcgcgccg gccgtgtgca ggtgatcgcc ccggccggct
      601 ttctgcacca cgcggtggcc gagaacgtgc tggccggcgt ggccatggcg cggcgctcgc
      661 agttccagtt cggccagacc ctgcccaagg gcccggccgg ccaggtggac gccggcctgg
      721 gcaagacact gccgctgggc cggccggggc tgatcgcgcc cacgcgcgag atcgcggccg
      781 agcacgaggt gctgacgatc gacggcctgg ccatcgagtt ccagctgacg cccgaaaccg
      841 aagcgccggc cgagatgcat ttgttcatcg ccagcgaggg cgcgctgaac ctggccgaga
      901 acgccaccca cacgctgcac aacctgtgcc cgctgcgcgg cgcccaggtg cgcgacgcgc
      961 tggcctggtc caagtacctg aaccaggccc tgcaccgcta cggcccgcgg accgaggtgg
     1021 tgctggccca gcaccactgg ccgacctggg gtcgcgatcg cgcctgccga ctgctggccg
     1081 aacagcgcga cctgtaccgc ctgctgcacg accagaccgt gcgcctgatg agccatggcc
     1141 tgaaagccgc cgagatcgcc gaaggcctgc agctgccgcc ggcgctggcc gcacgctggc
     1201 acacgcgtgg cttctacggc acggtgtcgc acaacgtgaa ggccatctac cagcactacc
     1261 tcagctggta cgacgccaac ccggccaacc tgcacgcgct gccgccggtg gaggccgcgc
     1321 gcaagaccgt ggcctatatg ggcggcgccg acgccctgct ggcgcgcgcg cgcgacgact
     1381 ttgcgcgggg tgaattccgc tgggtggccg aggtgatgaa acagctggtc tacgcccagc
     1441 ccgacctggc gccggcacgc gagctggcgg ccgcggcgct ggagcagatg ggcttccagg
     1501 ccgaatcggc gacctggcgc aacgcctttc tgctgggcgc gcgtgaatac cgcgacggcc
     1561 cgccgccacc cccgccggcc ggcgcgggca tggccatgct gggggcgctg gacaacgagc
     1621 tgctgttcga cgcactggcc gtgcgagtgc acgccggccg ggccgaaggg ctggccttca
     1681 cgctggcctg gcacttcacc gaccgcgacg agctgtggct gagcacgttg tcgcacggcg
     1741 ccttgagcag cctgcgcatc gccgacccag ccgtcgccgc cgcggccgac ctgcggatca
     1801 ccacagaccg gccgacgctg gacgccatcc tgcagcaacg cctgccgccg ccgcaggccg
     1861 tcagcgaagg gcggctgcag ctgcagggcc aggccgcgct gctgccgcgc ttcttcggcc
     1921 tgctggaccg cttcagcggc agcttcgcgg tggtcgacgc cgcgccctgg ccgctgtgac
     1981 gacaacaacc atcgaggaga cttctgatgt ccagccctga cacgccgcgt accggccgca
     2041 ccatcgccga gtcggtgccg cactggccgg cccggccgca gcgcccggcg ggtgcgccca
     2101 acatcctggt gatcctgttc gacgatgtcg ggttctctga cttcgggtgt tatggttcca
     2161 gcattcgcac gcccaacatc gacggcctgg ccgcgcaagg gctgcgctac accggctttc
     2221 acaccacggc catgtgttcg accacgcgcg cggcgctgct gaccgggcgc aaccaccatg
     2281 ccgtgggcat gggctgcctg gccaacttcg acagcggctt cgacggttac cgcggcaaga
     2341 tcagcccggc cgccggcacg ctggccgaaa tgctgcgccc gcagggctgg cgcaactaca
     2401 tggtgggcaa gtggcatgtc acgccgctga cgcagaccgg cccggccggc ccttacgacg
     2461 gctggccgct gggccgcggc ttcgaccgct actacggctt catggacgcc gagaccgacc
     2521 aatacgcgcc cgagctggtg cgcgacaaca cccacgtcga cccgccggcc acgccggacc
     2581 agggctatca cctcacggcc gacctggtcg acgaagccat ccgcatgctg gccgaccacc
     2641 aggccgatgc gcccgatgcc ccctggctgc tgtggctggc gctgggcgcc tgccatgcgc
     2701 cgcaccaggc gccgcaggac ctgatccgca gctacgacag ccagttcgcc catggctggg
     2761 atgtcgagcg cgaacagcgc ctggcgcgcc agaaggcgct gggcctggtg ccggccgaca
     2821 ccgaactgcc gccgcgcaac gacggcgtgc cggcctggga cacgctgggc gaggccgagc
     2881 gccgtgtctt cacgcggctg caatcggcgt ttgccggcat gctggaccat gccgaccagc
     2941 acatcggccg gctgctggcc tttctgcgcg acgcgcggct ggacgacgac accctggtgc
     3001 tggtgctgtc ggacaacggc gccagccagg aaggcgggcc gctgggcttc gtcaacgcca
     3061 tggggccgta caacctgaag cccgaggaca tggcggagaa actgcgccgc atcgacgaca
     3121 tcggcggcat cgacacccat tccaactttc cccaaggctg ggcgatggcg tcgaacacgc
     3181 cgctcaagcg ctacaagcag aacacccacg gcggcggcat tcgcgacccg ctggtgatgg
     3241 cctggcccaa gggcatcagc gcgcgcggcg aactgcgcca ccccttctgc cacgccagcg
     3301 acctgctgcc caccttgctg gagctgtgcc gcgtgcagcc gccggcgcag atcaacggcg
     3361 tggcgcagct gcccatcagc ggccagagtt tcgccgccag cctggccgac cccgcggcgc
     3421 cggcgcgcgc cacgccgcag tacttcgaga tgttcggcca ccgcggcatc tggcaccagg
     3481 gctggaaagc cgtggcctgg cacgcgccgg gcacgccctt tgacgccgac cgctgggagc
     3541 tgtaccacct ggccgaagac tttgccgaga accacaaccg cgccgacagc gagcccgaac
     3601 gcctgaaggc gctgcaggac ctgtggtggc gcgaggccga ggccaaccag gtgctgccgc
     3661 tggacgaacg tttcggcgca cgttttgccg agaacgcccg ccgcagccag ggcgctcgct
     3721 cgcagttcgt ctttcacgcc ggcatgggcc acctgcccac cgacgtggcg cccgacgtgc
     3781 gatcccgcag ctatcgcatc gaagccgagc tgctgatccc cgaaggcggc gccgaaggcg
     3841 tgctgatcgc ccatggcgac gccacctcgg gctacagcct ctacctgcaa ggcggccacc
     3901 tggtgcacga cctcaacatc ggtggcctgc accagttgct gcgttcggac cgggcggtgc
     3961 cgccagggcg gcggcggctg gccttcagca tgacgctggg accgatggtg ctgacgccgc
     4021 cgattcaagg cctgggccgg gtggaggtgc cgtccagccg gcgcggcagc ctgttcatcg
     4081 acggcgagcc ggtgggcgaa acgctgttca aggtgggctt cacctcgctg atctcgtggt
     4141 cgggcctgga catcggccgc gaccgcgcca gcccggtctc gcactacgcc gcgccgtttg
     4201 aattcaccgg ccggctgttc cacgtcacgc tgacgctggc gccgcagcag gcgctggccg
     4261 gcgccgccat cggccaggcc gagatggcgc ggcagtagcg gccgccgccg caccgccggc
     4321 ggcggtgctg gccgatcagc ccgggctgcc ggtctcgacc agtgacttca gccgttggaa
     4381 ctgctcggcc agcacgccgt tcaccggccc ggccagtttg tccagcgccg catcggccgg
     4441 gccgccgaca cggtagtgca ggcgcagcac cgtcttgccg tcctgctggc cgatgccgag
     4501 gttcagcacg ccttgcaccg gcaggtcttg cagcgggccc aggccgccgt gcaggcgcag
     4561 cagccgcccc ggcatcacca gcaccacctg ggcgtgctgc accgcgccgc cgtcccagcg
     4621 ttcgcaccag cagccgccgg cgcgcaggtc cagcgacagg ttgtcggctt tgcccgaata
     4681 ggtgtggctg ccgttccacc agcgcggcag ctgcacgatg gcctgccaga cggcgtcggg
     4741 gctggctttg acctcgtcgc gaaagctggc gacgaagccc tgggccgaga cctggctcag
     4801 ctcggcctgc gcagccagcg gcagcagggc ggccagcgcc agcagcgtgg cgcgcttcat
     4861 tgccaggccc ccaggatggc acccatcgcg gtgaaggcca gtaccaggta gccgctgttg
     4921 atgagccaca gcttgaacga gcggcgctcg aacaggtaga gcacgcccag cgacatcgcc
     4981 acccagccca ggccggtggc aaagcccgcg aagaccgcaa agcccaggct ggccttggcg
     5041 ccgatgaacg ccgccaggtt gaaggccatc accagcgcgg ccagaaaggc caggccgaag
     5101 atgcgggcca tcggcgcctg acgcagctgt tcgtcgctga gcccggcctc gcgctgccag
     5161 gcgcgcgaga atgccagcgg cgagtaccac aggccgccga tcgcgaagtg caccaccgcg
     5221 gcggccagca cggcggcata actgaactgc agcttgtcca tgtcgttctc cggttcgttc
     5281 cagcggccgg cgccgtcctc cggggcgacc cgatccgaag cccatggtgg ggccgcaccc
     5341 ggggccgacg caagcccgcc ggcacccaaa gccgacggcg gccggctcga agcggacaac
     5401 gcgctgccga atccgccgcc ggcccaggcc gcgcgccgcc ccggcgtgcg ggtcgatgac
     5461 cggcgcgtga tgctggccgg ctcgctgggc tttggcctgc tgatcccgca gctcagcggc
     5521 ctgttcggca gcctgggccc gggcgacctg cgcttctggg ccgggcaggc agcctacctg
     5581 gcactggccc tggccgtctg ggccggcaac cgctggctgc tgttccggca gcgcgaacat
     5641 ttcgactggt tcggccagcc ctggcgcaag ctggtgatgc tggtggtggc caacgtgttg
     5701 tacacggcgc cggtcacggt gctggggctg tggctggccg cgctgctgat gggccagccg
     5761 ctgccgcgcg cggcgctgca gctggtggtg gcggtcaacg tgatctgcgt gctgttcgtg
     5821 acccatgcct acgagacgct gttcctgatc cgcgagcgcg agtccgacct gatgtgggtc
     5881 gaacggctgc agcgggcccg cgcccaggcc gagctggctg cgctgaaagc ccaggtcgac
     5941 ccgcatttcc tgttcaacag cctgaacacg ctgggccatc tgatcagcca cgacagcgcc
     6001 cgcgcgcgtg aatgctgcga cacgctggcc gagatctacc gctacgtgct ggccgcgcgc
     6061 gatcacgatc tggtgcctct ggccgacgag ctggcgtttc tgcgccagta ccagcgcctg
     6121 ctggaactgc gctttgacca ggccgtgcag ctgcagctcg acccggcgct gcaggctgcc
     6181 ggcgcgcgct ggctgctgcc gccgctggct ttgcagacgc tgctggagaa cgccgtcaag
     6241 cacaaccgca tggcgcagga cgagccgctg cggctgcggc tgggcctgca gggcgattgg
     6301 gtggtcgcgc acaaccgccg ccggccgcgc agcagcgcgc tgccgggcag cggacgtggc
     6361 ctggccaacc tggacgaacg ctgccggctg ctgagcgggc gcgcattgcg cgtgcgccgc
     6421 agcgagcagg acttcgagct ggacctgccg ctggtggccg ccccatgaag ctgttcatcg
     6481 ccgaagacga agcaccggcg cgcgaacgcc tgctggagct gatcggccgc gtggcgcccc
     6541 aggccgtgct gctgggccat gccgacagtg tgcaggccac ccgcgactgg ctggccgcgc
     6601 acgcaccgcc cgacctgctg ctgctggaca tccagctggc cgacgggctg tcgctggagc
     6661 tgttccgcga ccgtgcgccc gactgcccgg tgatcttcac caccgcctac gaccagtttg
     6721 cactgcaggc ttttcaggcg ctggcagtgg actacctgct caagcccatt gccgacgcgg
     6781 cgctggcccg cgcctttgcc aaggccgggc gactgcgcgc cggttttgcg gccgacgtgg
     6841 ccggctggct ggccacgtcg ggcgtaccgg cggtggtgta ccgccagcgc ttgctgggcc
     6901 gccagggcgg ccagccgatg cacgtgctgc cggtggcgca gctggcctac ctggtctcgg
     6961 tcgacaaact ggccttcgcc gtgatggctg acggtcgacg ttacctgctg gacggcacgc
     7021 tcgccgacct ggcgctggaa ctggaccccg cgcgcttctt ccgcgccagc cgccaggtgc
     7081 tggtggcggc gtcggccatt gccgcttaca gcccggccgg caaaggccgg ctgcggctgg
     7141 acctgcggcc gcggctggac gacgagttgc tggtcagcca ggagcgcgcc gccgagttca
     7201 aggcctggct gcagcaggcg ccggcttagc cgctgcctct tgagcctcgc ccgcgctggc
     7261 ggcctgcgat cggggcttga cagtgagcgg gtcgcgctcg atactgaacc ttatggttaa
     7321 gtatgagcgc gcggcgctgg accccgtgtt tgcggcgctg gccgacggca cgcggcgcga
     7381 ggtgctggac cgactgggcg gggggccgct gagcgtcagc gagctggcgg ccggccatgc
     7441 catgtcgctg cccggtttca tgaaacacct ggcggtgctg gaagcggccg ggctgatcgc
     7501 gcgcgccaaa caaggccgcg tggtgcagtg cgaactggcg gccgggccgc tgcaggacgc
     7561 tgccgcctgg attgttcgtt atcaatcgtt ctggaccggc cggctcgacg cgctggaccg
     7621 ttacctgcac tcccagggag atcttgcgtg tcccccaacg cccccgtcga acccgccccc
     7681 gccgaacccg cagccgtcca cctgcgccgc cactacgccg ccgcgccgca agcggtctgg
     7741 cgcgcctgga ccgacccgca ggcgctgatg gcctggttcg gccccgccgg cccgggcacg
     7801 gcgacggtgg ccgagatgga cctgcaggtc ggcgggcgtt accgcttcgc cttcagcacg
     7861 cccgacggcg aggcgcacga ggtcagcggt gtctaccagg aggtgcagcc accgtttcgc
     7921 ctggtcttca gctgggcctg gcacagcacg cccgagcggg tgtcgcgcgt cacgctcgac
     7981 ctgcagccgg ccgacggcgg ttgcgagctg cgtttcaccc acgaccgctt cttcgacgaa
     8041 cgggcgcgcg ccaatcacca gcgcggctgg ccctcgttcc tgcagcgcct ggacggctgg
     8101 ctgagtgcca cgccgagcgg atcgccttct tctgtcacca ccccaaggac ctgtgaatga
     8161 gcacctctca cggcagctgc cactgcggcc gcatccgctt tgaagtcgac ggcgcgcccg
     8221 ccggcgtgat ggcctgcaac tgttcgatct gccagcgcaa aggcgcgctg atgtggttcg
     8281 tgccgcgcga gcagctgcgc ctgttgacgc cggaagcgga cagcagcgtc taccgcttca
     8341 acaaacacgt gatccagcac cgcttctgcc cggtctgcgg cattcacccc tacggcgaag
     8401 gcaccgaccc caaaggccag accatggcgg cggtgaacgt gcgctgcctg gaagacgtgg
     8461 acctggcggc gctgcccgtc accgcgttcg acggccgcgc gttgtaagac ggaaggcggc
     8521 tgcgcggccc tcacagcccg tcgaagcgat agcccacgcc gtagatcgag cgaatggtct
     8581 cctggcccgg cagcagccgc tccagcttgc ggcgcaggtt cttcacgtgg ctgtcgatgg
     8641 cgcggtcgct caatgcccgc tggtcgtcgg gcatagattc ttttgctgtg agttctttgc
     8701 ctttcgcgaa gaagatcagg ca
//
LOCUS       DAOQTZ010000103         8700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_8980_length_8700_cov_13.833083, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000103 DAOQTZ010000000
VERSION     DAOQTZ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8980_length_8700_cov_13.833083"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1532)
                     /gene="trpE"
                     /locus_tag="PKN51_11100"
     CDS             complement(33..1532)
                     /gene="trpE"
                     /locus_tag="PKN51_11100"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNT85689.1"
                     /translation="MITELEFKSLAGQGYNRIPLMSEAFADLETPLSLYLKLAGGPGG
                     GKYSFLLESVIGGERFGRYSFIGLPARTVLRASGFGEQARTEVVTDGRVVETQGGNPL
                     DFIDAYQRRFKVALRPGLPRFCGGLAGYFGYDVVRHIEKKLEQSCPPDTLGTPDILLM
                     HCEELAVIDNLSGKLYLIVYADPAEPEAYTRAKKRLKQLREQLRYSVAAPQVKASTNY
                     PAERGFAKADYLAAVERAKELIAAGDFMQVQVGQRISKRYTESPLSLYRAVRSLNPSP
                     YMIYYDFGDFHVVSASPEILVRQEHTPEGTKITIRPLAGTRPRGATPEQDKAIEQELV
                     NDPKERAEHVMLIDLARNDIGRIAKTGSVKVTEAFAVERYSHVMHIVSNVEGLLKDGL
                     SAMDVLKATFPAGTLSGAPKVHAMELIDQLEPVKRGIYGGACGYWSYAGDMDLAIAIR
                     TAIVKDQMLHVQAAAGVVADSVPEMEWRETEHKARALLRASELVEEGLE"
     gene            1881..2465
                     /locus_tag="PKN51_11105"
     CDS             1881..2465
                     /locus_tag="PKN51_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNT85690.1"
                     /translation="MRAAVRTLGLSALLLWAGTALAAQEIAGVKVEDAVTVAGTRLQL
                     NGSGVRYKGPFKVYVGDLYATRPVRSLDELIAAPGPKRLGMTFLREIQSAEFGKMLTR
                     GIEDNVSKTELSKIIPGMIKMGDIFAANKAFVPGDVCVLEWDPAKGLSIWAKGKLQAE
                     PMNDPAFYRALMSIWFGPTPADWKLKDALLGIKS"
     gene            complement(2455..3177)
                     /gene="gph"
                     /locus_tag="PKN51_11110"
     CDS             complement(2455..3177)
                     /gene="gph"
                     /locus_tag="PKN51_11110"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655418.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNT85691.1"
                     /translation="MSAPRGIDAAIIDLDGTMVDTLGDFVAALGRMLDDLGLPGVDAA
                     VVGRLVGKGSEHLLRSVLNHVLALQGQAPQATEIEALYPRAWARYQHHYLAINGRCAS
                     VYPGVVAGLTALRAAGLRLACLTNKPTAFALPLLRAKGLDGYFDQVFGGDAFARKKPD
                     PLPLLKTCEALGTPAARTLMVGDSSNDAAAARAAGCPVVLVTYGYNHGQPVREVDADG
                     FVDALHELLPTWLCGRPAGVRT"
     gene            complement(3425..4105)
                     /gene="rpe"
                     /locus_tag="PKN51_11115"
     CDS             complement(3425..4105)
                     /gene="rpe"
                     /locus_tag="PKN51_11115"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNT85692.1"
                     /translation="MSTTYRIAPSILSADFARLGQEVRDVIAAGADWIHFDVMDNHYV
                     PNLTFGPMVCQAIKPHAVTPDGRPVPLDVHMMVQPVDALAQAFAQAGADLISFHPDAS
                     AHAHRSVQAIKAAGCQAGLVFNPAAPLDVLDWLIDDIDLILIMSVNPGFGGQSFIDSA
                     LRKVEAARRRIESSGRDIRLEVDGGIKASNIRQVADAGADTFVAGSAIFGQPDYRAVI
                     DAMRRAMA"
     gene            4139..4534
                     /gene="apaG"
                     /locus_tag="PKN51_11120"
     CDS             4139..4534
                     /gene="apaG"
                     /locus_tag="PKN51_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNT85693.1"
                     /translation="MPTYQFHVEVQPEYLPEQSEPAHSRYGFAYTITVLNTGQVAAQL
                     VSRHWIIVDGRGHTEEVRGLGVVGQQPLLQPGEAFQYTSGCQLRTPSGTMHGSYFCVA
                     EDGERFEVAIPLFVLDASGADGGSARVLH"
     gene            4586..6619
                     /locus_tag="PKN51_11125"
     CDS             4586..6619
                     /locus_tag="PKN51_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HNT85694.1"
                     /translation="MAPPRPDLPELLHALDPLAPRAERHLWLMQLLDWVRGQQDEPQA
                     AVARVRMLLDVAEQRPDWLGLWRHWWATFLTEVDATPLLADLGFAARSAFLAELSDRL
                     RRQLIPPTPETTDLRELFALLLPGEFDARWLRALDKPTLERLRRTLFDDAPAEGAPPY
                     ALLTLLNALAYAISQISAAGQSAELRIRMAPEVQRQQPFAALPIGFEALRQAVLSHGR
                     DSAPALVALAQLREQLDACRAAAASVYAHLEEHGISVHIEFQLRQLRQRIIRVKALLL
                     CLETDEPAQATAQLLSHLVRVQLDSRSVGALVRSSTELLAARVTERNAETGEHYIARD
                     RREYLHMLGAAAGGGAVMALTTLSKLVIVGLGLSAFWGGVAAGLNYAISFVIIMLLHW
                     TVATKQPAMTAPTLAARLKALDTPAAHEAFVTEVVHLLRTQFVAIVGNVVVVLALSFA
                     LVAAIGALGGGPWIDDAHAHHILDEHHMLGRTIPFAIVTGGLLFASSLIAGWVENWFV
                     LHRLDSVIAHHPRSTRLLGRERARRWGRYWGSHISGWAANVSLGLLLGLVPPIVGFFG
                     LPVEVRHVTLVSGQIAVAAQQLGPAVWQDPALWWAAGGIAVTGLLDVGVSFYLAFRLA
                     LTAQNVAGVDRRRIYQAMGRRLWRDPWSFVLPVRERPPATQAPADPHKPGQTG"
     gene            6727..7995
                     /locus_tag="PKN51_11130"
     CDS             6727..7995
                     /locus_tag="PKN51_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNT85695.1"
                     /translation="MTEPWIAHWTSGSELSAGLITLKWALLLAAAALAGHLSQRLLGL
                     PKLVGYAVFGTLAGLTGLAGADWPLQGAGLTLVELGVAMLLFEAGARLSLRWLRHNPM
                     VLWQSVVESLLTYAAAYLLLRALGLELPVVRALSVVAVASSPAVLLRVVSDLRASGPV
                     SDRAIALATLNTLYALTLGTAMLRSIDRGDVSLLASLGASATVLGISLLTGAALASLL
                     RLAFRVLHPASQDTAIVILALLAACVAVATPLGGSAPLAALLAGLVLKRLYPRPWVWP
                     RQLGTAASMLSMAMFVLVSTLAAQASWSAASALAALGLTGARLLAKLASLLLTGPVSA
                     LSLRQSAWVGVALFPTSAVALLLTSQFVAAAPTVGTPVAAIALPVILLTELLGAALVG
                     LALVRTREAPAPRRWREPAPADTTPPETRP"
     gene            7992..>8700
                     /locus_tag="PKN51_11135"
     CDS             7992..>8700
                     /locus_tag="PKN51_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HNT85696.1"
                     /translation="MTLEAFHTSEALTLGVELELQLVNTNDYDLTHYADNMLRLMARR
                     DLPGSVVPELTTGMIEVSTGICHDWHEARAQLQQIRDALVHSADKLGIAVVGGGTHPF
                     QQWPQQRIYDKPRFHELSQLYGYLSKQFTIFGQHVHVGCPDADSALYTLHCLSRYIPH
                     FIALAASSPFVQGQDTAFDSARLNSVFAFPLSGRAPCVLSWAEFGQYFDKMTRTGVVR
                     SMKDFYWDIRPKPEYGTI"
BASE COUNT         1200 a         3057 c         3064 g         1379 t
ORIGIN      
        1 catgagcagc tggatctttt tcgccatggc gctcactcca gtccttcttc caccagctcg
       61 ctcgcccgca gcagggcgcg cgccttgtgc tcggtctcgc gccattccat ctcgggcacg
      121 ctgtcggcca ccacgccggc cgccgcctgc acgtgcagca tctggtcctt gacgatggcg
      181 gtgcgaatcg caatggccag gtccatgtcg ccggcgtagc tccagtagcc gcaggcgccg
      241 ccgtagatgc cgcgcttgac cggctccaac tggtcgatca gctccatggc gtgcaccttg
      301 ggtgcgccgc tcagggtgcc ggccgggaag gtggccttga gcacgtccat ggcgctcagg
      361 ccgtccttga gcagaccctc gacgttgctg acgatgtgca tcacgtggct gtagcgctcc
      421 acggcaaagg cctcggtcac cttgacgctg ccggtcttcg cgatgcggcc gatgtcgttg
      481 cgcgccaggt cgatcagcat cacgtgctcg gcgcgctcct tggggtcgtt gaccagctcc
      541 tgctcgatcg ccttgtcctg ttcgggcgtg gcgccgcgcg ggcgcgtgcc cgcgagcggc
      601 cggatcgtga tcttggtgcc ctcgggcgtg tgctcctgtc gcaccaggat ctcgggactg
      661 gcgctgacca cgtggaagtc gccgaagtcg taatagatca tgtacggcga cgggttgagc
      721 gagcgcaccg cgcggtacag cgacaggggc gattcggtgt agcgcttgct gatgcgctgg
      781 cccacctgca cctgcatgaa gtcgccggcg gcgatcagct ccttggcgcg ctcgaccgcc
      841 gccaggtagt cggccttggc aaagccgcgc tcggccgggt agttggtgct ggccttgact
      901 tgcggcgccg ccaccgaata gcgcagctgc tcgcgcagct gcttgagccg cttcttggcg
      961 cgggtgtagg cctcgggctc ggccggatcg gcgtagacga tgaggtagag cttgcccgac
     1021 aggttgtcga tcaccgccag ctcctcgcag tgcatcagca ggatgtcggg cgtgcccagg
     1081 gtgtcgggcg ggcagctttg ctccagcttc ttctcgatgt gacgcaccac gtcgtagccg
     1141 aagtaacccg ccagcccgcc gcaaaagcgc ggcaggccgg ggcgcagcgc gaccttgaag
     1201 cgccgctggt aggcgtcgat gaagtccagc gggttgccgc cctgcgtctc gaccacccgg
     1261 ccgtcggtga cgacttcggt acgcgcctgc tcgccaaaac cgctggcgcg cagcacggtg
     1321 cgcgccggca ggccgatgaa gctgtagcgg ccgaagcgct cgccaccgat gaccgattcg
     1381 agcagaaagc tgtacttgcc gccgcccggc ccgccggcca gcttgaggta cagcgacagc
     1441 ggggtttcga ggtcggcaaa ggcctcgctc atcaggggga tgcggttgta gccctggccg
     1501 gccaggctct tgaattccag ttcggtgatc acaggagact cccagatcgg gcggcgtcgg
     1561 cgcgccgcgg gttcattcgg gggtggttgg ccgcgcgacc tgcaacaggc ggggcctcgg
     1621 aaagcaggcc ggacaacccg gctgcggatc aggggttcag cagcgcaggc ttgcgccagg
     1681 gccaggctcc ctggcccccg tgacctgcga tgaacgcgct gggaatgaac atgggcgcag
     1741 tgtagcaaac gtgaacccgg tcacggatcg ccgcgctggc acgtcagggg ctggggcaaa
     1801 ccccgatgca ggccatggcc agttcactaa actttagtcg tcaattcgaa cgatcgtgct
     1861 ttttcaaagg aggttctccc atgcgcgctg ccgtacgcac ccttggtctg tctgccctgt
     1921 tgctgtgggc gggcaccgcc ctggccgcgc aggagatcgc cggcgtcaag gtggaagacg
     1981 ccgtcaccgt ggccggcacc aggctgcagc tcaacggcag cggcgttcgc tacaaggggc
     2041 cgttcaaggt ctacgtgggt gacctgtacg ccacgcgccc ggtgcggtcg ctggacgagc
     2101 tgatcgccgc gcccggcccc aagcgtctgg gcatgacctt tctgcgcgag atccagtcgg
     2161 ccgagttcgg caagatgctc acacgcggca tcgaggacaa cgtcagcaag accgaactct
     2221 ccaagatcat ccccggcatg atcaagatgg gcgacatctt tgccgccaac aaggccttcg
     2281 tgccgggcga cgtgtgtgtg ctggaatggg acccagccaa gggcctgagc atctgggcca
     2341 agggcaagct gcaggccgag ccgatgaacg atccggcctt ctaccgtgcg ctgatgtcga
     2401 tctggttcgg ccccaccccg gccgactgga agctcaagga cgcgctgctg ggcatcaagt
     2461 cctgacgccg gccggtcggc cgcacaacca ggtcggcagc agctcgtgca gggcgtcgac
     2521 gaagccgtcg gcgtccacct cgcgcaccgg ctggccgtgg ttgtagccat aggtgaccag
     2581 caccaccgga cagccggcgg cgcgcgcggc ggcggcgtcg ttgctggagt cgcccaccat
     2641 cagggtgcgc gccgcgggcg tgcccagggc ctcgcaggtc ttgagcagcg gcagcgggtc
     2701 gggcttcttg cgcgcgaagg cgtcgccgcc gaacacctgg tcgaaataac cgtccagacc
     2761 cttggcgcgc agcagcggca gcgcaaaggc ggtgggcttg ttggtcaggc aggccaggcg
     2821 cagaccggcc gctcgcagcg ccgtcagccc cgccaccacc cccggataga cgctggcgca
     2881 gcgaccgttg atggccaggt agtggtgctg atagcgcgcc caggcccggg gatagagcgc
     2941 ttcaatttct gtagcttgcg gcgcttgtcc ctgcaacgcc agcacgtgat tgagcacaga
     3001 acgcagcagg tgctcggagc ccttgccgac cagccgcccg accaccgcgg cgtcgacgcc
     3061 gggcaggccc aggtcgtcca gcatccggcc cagggcggcg acgaaatcgc ccagggtgtc
     3121 gaccatggtg ccgtcgagat cgatgatggc ggcgtcgatg ccgcgcggcg cgctcactgg
     3181 acgaccctcc gcacggcgtg ctgcgggatg cgcgcttggg agcggcccgg cgcgcttatt
     3241 cggtgtccct tcgcgctgac gcgctgcggg attcgcgctt gggagcggcc cggcgcgctc
     3301 attaggtgtc ccttcgcgct gacgcgctgc gggattcgcg cttgggagcg gcccggcgcg
     3361 ctcatagggt gtcccttcgc gctgacgcgc tgcgggatgc gcgcttggga gcggcccggc
     3421 gcgctcatgc catcgcgcgc cgcatggcgt cgatgacggc gcggtagtcc ggctgaccga
     3481 agatggcgct gccggccacg aaggtgtcgg cgccggcgtc ggccacctgg cggatgttgc
     3541 tcgccttgat gccgccgtcc acctccagcc ggatgtcgcg gcccgaggac tcaatgcggc
     3601 ggcgcgccgc ttccaccttg cgcaaggcgc tatcaataaa ggactgaccg ccaaagccgg
     3661 ggttgacgct catgatcagg atcagatcga tgtcgtcgat cagccagtcc agcacatcca
     3721 gcggcgccgc cgggttgaac accagaccag cctggcagcc cgccgccttg atcgcctgca
     3781 cgctgcggtg ggcgtgtgcg ctggcgtcgg ggtgaaagct gatcagatcg gcgccggcct
     3841 gggcaaaggc ctgggccagc gcgtccaccg gctgcaccat catgtgcacg tccagcggca
     3901 ccggccggcc gtcgggcgtg acggcgtgcg gcttgatggc ctggcagacc atcggcccga
     3961 aggtcaggtt gggcacgtaa tggttgtcca tcacgtcgaa gtggatccag tcggcgccgg
     4021 cggcgatcac gtcgcgcacc tcctgaccca gccgtgcgaa gtcggccgaa aggatggagg
     4081 gcgcaatgcg gtaggtggtg ctcatggcgc cggattgtcg cgcaggtacc atgcgcgcat
     4141 gccgacctac cagttccacg tcgaggtgca gcccgaatac ctgcccgagc agtccgagcc
     4201 ggcgcacagc cgctacggct ttgcctacac catcaccgtg ctcaacaccg gccaggtggc
     4261 ggcgcaattg gtgtcgcgcc actggatcat cgtcgacggg cgcggccaca ccgaggaggt
     4321 caggggcctg ggcgtggtcg gccagcagcc gctgctgcag ccgggcgaag ccttccagta
     4381 caccagcggc tgccagttgc gcacgcccag tggcaccatg cacggcagct atttctgcgt
     4441 cgccgaggac ggcgagcgct tcgaggtggc gatccccctg ttcgtgctgg acgccagcgg
     4501 cgccgacggc ggcagcgcgc gcgtgctgca ctgagtgcgt gggcacgctc cccacatgcc
     4561 caccctctga acctgcgcac acccgatggc accgccccgg cccgacctgc ccgaactgct
     4621 gcacgcgctc gacccgctgg cgccgcgcgc cgaacgccat ctgtggctga tgcagctgct
     4681 cgactgggtg cgcggccagc aggatgagcc gcaggccgcg gtggcgcggg tgcgcatgtt
     4741 gctggacgtt gccgaacagc gccccgactg gctggggctg tggcgccact ggtgggccac
     4801 cttcctgacc gaggtggacg ccacaccgct gctggccgac ctgggttttg ccgcgcgcag
     4861 cgcctttctg gccgagctgt ccgaccgcct gcggcgccag ctgatcccgc cgacgccgga
     4921 aaccaccgac ctgcgcgagc tgttcgcgct gctgctgccg ggcgagttcg acgcccgctg
     4981 gctgcgcgcg ctggacaagc cgacgctgga gcggctgcgc cgcacgctgt tcgacgacgc
     5041 cccggccgaa ggcgccccgc cctacgccct gctcacgctg ctgaacgcgc tggcctacgc
     5101 catcagccag atcagcgccg ccggccaatc ggccgagctg cgcatccgca tggcgcccga
     5161 ggtgcagcgc cagcagccgt tcgccgccct gccgatcggc ttcgaggcgc tgcgccaggc
     5221 ggtgctgagc cacggccgcg acagcgcacc ggcgctggtg gcgctggcgc agttgcgcga
     5281 gcagctcgac gcctgccgcg ccgccgccgc cagcgtgtat gcgcacctgg aagagcacgg
     5341 catctcggtg cacatcgaat tccagctgcg ccagctgcgc cagcgcatca tccgcgtcaa
     5401 ggcgctgctg ctgtgcctgg agaccgacga gccggcgcag gccacggccc agctgctgtc
     5461 gcacctggtg cgcgtgcagc tggacagccg cagcgtgggc gcgctggtgc gcagcagcac
     5521 cgagctgctg gccgcgcggg tgaccgagcg caacgccgag accggcgaac actacatcgc
     5581 ccgcgaccgg cgcgaatacc tgcacatgct gggcgcagcg gcgggcggcg gcgccgtgat
     5641 ggcgctgacc acgctgagca agctggtgat cgtcggcctg ggcctgtcgg ccttttgggg
     5701 cggggtggcg gcggggctga actacgccat ctccttcgtc atcatcatgc tgctgcactg
     5761 gacggtggcc accaagcagc cggccatgac ggcgcccacc ctggcggcgc ggctgaaggc
     5821 gctcgacaca cccgccgcgc acgaagcatt cgtcaccgaa gtggtgcatc tgctgcgcac
     5881 gcagttcgtg gccatcgtcg gcaacgtggt ggtggtgctg gcgctcagct ttgcgctggt
     5941 ggcggcgatc ggcgcgctgg gcggcggccc ctggatcgac gacgcgcacg cgcaccacat
     6001 cctggacgag caccacatgc tggggcgcac catcccgttc gccatcgtca ccggtggcct
     6061 gctgttcgcc tccagcctga tcgccggctg ggtggaaaac tggttcgtgc tgcaccgcct
     6121 ggattcggtg atcgcgcacc acccgcgcag cacccggctg ctgggccgcg agcgcgcccg
     6181 gcgctggggc cgctactggg gcagccacat ctccggctgg gcggccaacg tctcgctcgg
     6241 tctgctgctg gggctggtgc cgccgatcgt cggcttcttc ggcctgccgg tggaagtgcg
     6301 ccacgtgacc ctggtgtcgg gccagatcgc cgtggcggcg cagcagctgg ggcccgccgt
     6361 gtggcaggac ccggccctgt ggtgggccgc cggcggcatc gccgtcaccg gcctgctgga
     6421 cgtcggcgtc agcttctacc tggcgttccg gctggcgctg acggcgcaga acgtcgccgg
     6481 cgtcgatcgc cgccgcatct accaggccat gggccggcgc ctgtggcgcg atccgtggtc
     6541 ctttgtgctg ccggtgcgcg agcggccgcc ggccacccag gcccctgccg acccccacaa
     6601 gcccggccaa acgggttagc atgggcgctg ccgcgccggc cctggtccgc gcggcctgcc
     6661 ggggccgccc ggggcgccac cagcgcgcgc agcggccgcc ggcaggcatc ggagacgctg
     6721 actgttttga ctgaaccatg gatcgcccac tggaccagcg ggtctgaact ctctgccggc
     6781 ctgatcaccc tcaagtgggc gctgctgctg gccgcggcgg cgctggccgg gcacctgtcg
     6841 caacgcctgc tgggcctgcc caagctggtc ggctatgcgg tgttcggcac cctggccgga
     6901 ctgaccggac tggccggcgc cgactggccg ctgcagggcg ccggcctgac cctggtggag
     6961 ctgggcgtcg ccatgctgct gttcgaagcc ggcgcccgcc tgtcgctgcg ctggctgcgc
     7021 cacaacccga tggtgctgtg gcaaagcgtg gtcgagtcgc tgctgaccta cgccgccgcc
     7081 tacctgctgc tgcgcgcgct ggggctggag ctgccggtgg tgcgggcgct gtcggtggtg
     7141 gcggtggcgt cgtcgccggc ggtgctgctg cgcgtggtgt ctgatctgcg cgccagcggg
     7201 ccggtgagcg accgcgccat cgcgctggcc acgctgaaca ccctgtatgc gctcaccctc
     7261 ggcaccgcca tgctgcgcag catcgaccgc ggcgacgtgt cgctgctggc gtcgctgggc
     7321 gcctcggcga cggtgctggg catttcgctg ctgaccggcg ccgcgctggc gtcgctgctg
     7381 cggctggcgt tccgcgtgct gcacccggcc agccaggaca ccgccatcgt catcctggcc
     7441 ctgctggcgg cctgcgtcgc cgtcgccacg ccgctgggcg gctcggcgcc gctggcggcc
     7501 ctgctggccg ggctggtgct caagcggctg taccccaggc cctgggtgtg gccgcgccag
     7561 ctcggcaccg ccgcatcgat gctgagcatg gccatgttcg tgctggtgtc cacgctggcg
     7621 gcgcaggcca gctggagcgc cgcctcggcg ctggcggcgc tgggcctgac cggcgcacgc
     7681 ctgctggcca agctggccag cctgctgctg accggcccgg tcagcgcgct gtcgctgcgc
     7741 cagtcggcct gggtcggggt ggcgctgttt ccgacgtcgg cggtggcgct gctgctgacc
     7801 tcgcagttcg tcgccgccgc ccccaccgtg ggcacgccgg tggcggccat cgcgctgccg
     7861 gtgatcctgc tgaccgagct gctgggggcg gcgctggtgg gcctggcgct ggtgcgcacg
     7921 cgcgaggcgc cggcgccccg gcgctggcgc gagccggcac cggccgacac cacccccccg
     7981 gagacacgac catgacgctg gaggccttcc acacctccga ggcgctgacg ctgggcgtcg
     8041 agctggagct gcagctggtc aacaccaacg actacgacct gacccactac gccgacaaca
     8101 tgctgcgcct gatggcgcgg cgcgacctgc cgggctcggt ggtgcccgag ctgaccaccg
     8161 gcatgatcga ggtgtcgacc ggcatctgcc acgactggca cgaggcgcgc gcccagctgc
     8221 agcagatccg cgacgcgctg gtgcacagcg ccgacaagct gggcatcgcc gtggtgggcg
     8281 gcggcacgca cccgttccag caatggcccc agcagcgcat ctacgacaag ccgcgctttc
     8341 acgagctgtc gcagctgtat ggctacctga gcaagcagtt caccatcttc ggccagcatg
     8401 tgcacgtggg ctgccccgac gccgacagcg cgctctacac cctgcactgc ctgtcgcgct
     8461 acatcccgca cttcatcgcg ctggcggcgt ccagcccgtt cgtgcagggt caggacacgg
     8521 ccttcgattc ggcgcggctg aactcggtgt ttgcctttcc gctgtcgggg cgcgcgccct
     8581 gcgtgctgag ctgggccgaa ttcgggcagt acttcgacaa gatgacccgc accggcgtgg
     8641 tcagaagcat gaaggacttc tactgggaca tccgccccaa gcccgagtac ggcaccatcg
//
LOCUS       DAOQTZ010000104         8687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_8991_length_8687_cov_14.776413, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000104 DAOQTZ010000000
VERSION     DAOQTZ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8991_length_8687_cov_14.776413"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1393)
                     /locus_tag="PKN51_11140"
     CDS             complement(167..1393)
                     /locus_tag="PKN51_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT85697.1"
                     /translation="MTTATAAPSPVSRNRLLVTSGLGWMFDAMDVGLLSFILAALKAD
                     WGLSAGQMGWLGAINSIGMAVGAIGFGMLADRIGRKQVFILTLLLFAIASGLSAFAST
                     LAVLFVLRFFIGMGLGGELPVASTYVSERVAAHERGRVVVLLESFWAVGWILAALIAY
                     FVIPRFAQDMGWRVALVLGALPAFYALYLRRQLPGDAPTAPPAAGLPWTARLAALWSP
                     AHARATLMLWLLWFTVVFSYYGMFLWLPSVMVGKGFALIKSFEYVLLMTLAQLPGYFT
                     AAWLIERAGRKFVLVVYLTGTAASAWAFGNADTQGALLLYGALLSFFNLGAWGALYAY
                     SPENYPAPIRASGVGTAAGIGRLGGILGPLTIPFLGAQGWGTGAIFGLFAATLLVGVL
                     AVAVLGRETRGAQVHA"
     gene            1463..3013
                     /gene="radA"
                     /locus_tag="PKN51_11145"
     CDS             1463..3013
                     /gene="radA"
                     /locus_tag="PKN51_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNT85698.1"
                     /translation="MASPKTVYTCHACGASSPRWLGKCPGCGAWNSLVEAVAEAATGG
                     RNRLGGAFAALAPSTAVRPLAAIEAVDVARSPTGQAELDRVLGGGLVAGGVVLIGGDP
                     GIGKSTLLLQALDSLQRAHPSTAGPPQGASAPSGGSATGAAVERGGRDPSTAGPPQGA
                     SAPSGGSATGAAAQRGGMLYVTGEESGAQVALRARRLGIAHSQVAVLAEIQLEKILAT
                     LSAQQPAVAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRWAKASGCAVVLVGHVTK
                     DGQLAGPRVLEHMVDTVLYFEGDTHSSYRLIRAIKNRFGAVNEIGVFAMTERGLKGVS
                     NPSAIFLAQHAEPVPGSCVLVTLEGTRPLLVEVQALVDSGGPSPRRLSVGLERDRLAM
                     LLAVLHRHAGVATGDQDVFVNAVGGVRISEPAADLAVLLAIASSLRGKPLPKGFIAFG
                     EVGLAGEVRPAPRGQERLKEAAKLGFSVALIPRANAPRKADPAFEGVTLHTVERVDEA
                     MALLRTLE"
     gene            3147..3572
                     /locus_tag="PKN51_11150"
     CDS             3147..3572
                     /locus_tag="PKN51_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNT85699.1"
                     /translation="MKWQRLWVMLGAVALGAGAWRAGGWAGLALLGSGLVLWFLLHYH
                     RLLTVMKRANERPIGHIGSAVMLHAKLRQGLPLLHVMALTQSLGERLSAPEAQPEVYR
                     WSDPGQSHVVTEFEAGRLVRWRLERPADPDVQASTPEAH"
     gene            3740..4672
                     /locus_tag="PKN51_11155"
     CDS             3740..4672
                     /locus_tag="PKN51_11155"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNT85700.1"
                     /translation="MSTPPLSDRDGKIWLDGQLVDWRDAKIHVLTHTLHYGCGAFEGV
                     RAYDTVGGTAVFRLQEHTQRLFNSAKILRMAIPFTPEQVNEAQKEVVRANQLKSCYLR
                     PLVWIGSEKLGVSPKGNRIHVMVAAWAWGAYLGEEGLKRGIRVKTSSYTRHHVNITMT
                     QAKAVSNYSNSILANMEATDDGYDEAVLLDSAGFVSEGAGENLFIIKDGTVYTPDLSA
                     GALNGITRNTVLHICEDLGLKVVQKRITRDECYIADEMFFTGTAAEVTPIREYDRVQI
                     GAGQRGPVTEKIQSAFFDIVNGRNAKYAHWLAKV"
     gene            4698..4907
                     /locus_tag="PKN51_11160"
     CDS             4698..4907
                     /locus_tag="PKN51_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HNT85701.1"
                     /translation="MSTPNQVVELLARDLTRSGETFCPHPKADMKLWNTHPRVFLNLA
                     QGEARCPYCGTLYRMKAGEVVGAGH"
     gene            complement(4952..6184)
                     /locus_tag="PKN51_11165"
     CDS             complement(4952..6184)
                     /locus_tag="PKN51_11165"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT85702.1"
                     /translation="MPQLPMVRWVALFWGFVAFFPIGLNYTAFVLLAVAMVWQGDRAE
                     RLARLRQAPQWWPAWALVGWSTLILLLGPHYAETPSNALHALRIVLTLALALALTRDE
                     ALWALRGVLLGLAVGLLLVALHGLVPLPPHPLWDSLVRYDGNKSLANAVQMAMVAAGS
                     LLLLPALGRRGQVLAALLAAAVMLVLIWVLHSRTAWLVLVLGLALGLWHRLRGRRRPQ
                     LAALATAALLTLAAGLLLPGVKQRLQQGAGELVSAYQGQTVEMDSSWGIRYRMYRETT
                     AMIAERPLLGWGIGGWNTQWRQRVNGPLTDVNMPHNDLLWMGAQTGVPGALLLLWLLL
                     AGWPAAWRRDDLNGSLAALALLAMLLAVLGNSALRDASMGLSLWFVVLVIQRLAHQPQ
                     PLWQGLWPAGAVAPQAGR"
     gene            complement(6427..7704)
                     /locus_tag="PKN51_11170"
     CDS             complement(6427..7704)
                     /locus_tag="PKN51_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT85703.1"
                     /translation="MKVTDSIARRLYLRIWLAVAGSVLVLSLLIGWGWHAAEQERERD
                     RQAGQPREVLVRNAAGELIGTAEAQPRRLPGQGFEFVVTLRDGQVLSLQMPPRGRGRA
                     AAGAAARRHADLPPSWLRPPYGFWWLLALAGLGVMLGVYPVARRLTQRLEALQRGVQR
                     WGEGDLSARLPESGQDEVADLSRRFNAAAERIQTLMDAQGALLQSQKSLLANASHELR
                     SPLARIRMAMALAGPQGSPAARAEIERSITELDQLVDEILLASRLDAREADLGTVEPV
                     DLIGLLAEECARVGAEFTLADGTRQVVVPGVARLLRRAVRNLLENAVRHGRRTGRDGE
                     VGAHLQLAGGAAELQVDDRGPGVPPEQRERIFEPFYRLPGASEREGGVGLGLALVRSI
                     AQRHGGSVTCDHRPGGGARFTLRLPVEADAATK"
     gene            complement(7790..8497)
                     /locus_tag="PKN51_11175"
     CDS             complement(7790..8497)
                     /locus_tag="PKN51_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT85704.1"
                     /translation="MSTHRLLMIEDDARLAAMVGEYLTQSGYAVTHRGDGETGLQTLQ
                     GEPFDLVVLDLMLPGIDGLEVCRRIRALPDGASRVPVLMLTAKGDPMDRIIGLELGAD
                     DYLPKPFEPRELLARIRAVLRRQQPGAEPEHRLLRFGTLEIDRDARTVSVGGQPAELT
                     SYQFDLLVTLAERAGRVLTREQIMEAVRGRELEAFDRSIDVHMGRIRAAIEQDVKNPR
                     RILTVRGVGYVFARQQD"
BASE COUNT         1440 a         3121 c         2999 g         1127 t
ORIGIN      
        1 gcaccacccg cgctccgctg cgccacgggc agatcgaatc aacaatataa aagtattatt
       61 gatgatggcc atccaggcca tgtggccgca agatcacgcg tgaattagtt cactcgtggc
      121 tcgttgtgga ccgaccacaa cggcaccatg gcgcagaccc gccaggtcag gcgtgtacct
      181 gcgcgccgcg cgtctcgcgc cccagcacgg cgaccgccag cacgcccacc agcaaggtgg
      241 cggcgaacag gccgaagatg gcgccggtgc cccagccctg cgcccccagg aaggggatgg
      301 tcagcggccc caggatgccg cccaggcgcc cgatgccggc ggccgtgcca acgccgctgg
      361 cgcggatcgg cgccgggtag ttttcgggcg aataggcgta cagcgcgccc caggcgccca
      421 ggttgaagaa cgacagcagc gcgccataca gcagcagcgc gccctgcgtg tcggcgttgc
      481 cgaaggccca ggcgctggcc gccgtgccgg tcaggtacac caccagcacg aacttgcgcc
      541 cggcgcgctc gatcagccag gcggcggtga agtagccggg cagctgcgcc agcgtcatca
      601 gcagcacgta ctcgaagctc ttgatgagcg caaagccctt gccgaccatg acgctgggca
      661 gccacagaaa catgccgtag tacgagaaca cgacggtgaa ccacagcagc cacagcatca
      721 gtgtggcgcg cgcatgcgca ggcgaccaca gcgccgccag acgcgccgtc cacggcaggc
      781 cggccgccgg cggggcggtg ggcgcatcgc cgggcagctg gcgccgcagg tacagggcat
      841 agaaggcggg cagcgcgccc agcaccaggg ccacgcgcca gcccatgtcc tgggcaaagc
      901 gcgggatgac gaagtaggcg atcagcgccg ccaggatcca gccgacggcc cagaagctct
      961 ccaacagcac caccacgcgg ccgcgctcgt gtgcggccac gcgctcggac acgtaggtgc
     1021 tggccaccgg cagctcgccg cccaggccca tgccgatgaa aaagcgcagc acgaacagca
     1081 ccgccagcgt gctggcaaag gccgacagac cgctggcgat ggcaaacagc agcagcgtca
     1141 ggatgaacac ctgcttgcga ccgatgcggt cggccagcat gccgaagccg atcgccccca
     1201 ccgccatgcc gatcgagttg atggcgccca gccagcccat ctgtccggcc gacaggcccc
     1261 agtcggcctt gagcgcggcc aggatgaacg acaacaggcc gacatccatg gcgtcgaaca
     1321 tccagcccag gcccgaagtg acgagcaggc ggttgcgtga gacgggcgac ggcgcggcgg
     1381 tggcggtggt catgagacga tgcaagcggg atcgggcctg gggtggcgca tggtacccgc
     1441 ccggcgccgt tacgattgcc ccatggccag ccccaagacc gtctacacct gccacgcatg
     1501 cggcgccagc agtccgcgct ggctgggcaa atgccccggc tgcggcgcct ggaacagcct
     1561 ggtcgaggcg gtggccgaag ccgccaccgg cggcagaaac cgtctgggcg gcgcattcgc
     1621 ggcattggcc ccatcgaccg ccgtgcggcc gctggccgcc atcgaggcgg tggacgtggc
     1681 gcgcagcccc accggccaag ccgagctgga ccgcgtgctg ggcggcggcc tggtggccgg
     1741 cggcgtggtg ctgatcggcg gcgaccccgg catcggcaaa tccaccctgc tgctgcaggc
     1801 cctggacagc cttcagaggg cgcacccgag caccgccggg ccgccccaag gtgcgagcgc
     1861 cccctcgggg ggcagcgcaa caggcgcagc cgtggagcgt gggggccgtg acccgagcac
     1921 cgccgggccg ccccaaggtg cgagcgcccc ctcggggggc agcgccaccg gcgccgcggc
     1981 gcagcgcggg ggcatgctgt acgtcacggg cgaagaaagc ggcgcccagg tggcgctgcg
     2041 cgcgcggcgt ctgggcattg cccactcgca ggtggcggtg ctggccgaga tccagctgga
     2101 aaaaatcctc gccaccctga gtgcacagca gcccgcggtg gcggtgatcg actcgatcca
     2161 gaccgtctac agcgaccagc tcaccagcgc cccgggcagc gtggcccagg tgcgcgaatg
     2221 cgccgcccac ctgacgcgct gggccaaggc cagcggctgc gccgtggtgc tggtgggcca
     2281 tgtgaccaag gacggtcagc tggccggccc gcgcgtgctg gagcacatgg tcgacaccgt
     2341 gctgtacttc gagggcgaca cgcacagcag ctaccgcctg atccgcgcca tcaagaaccg
     2401 ctttggcgcc gtcaacgaaa tcggcgtgtt cgccatgacc gagcgcggcc tgaaaggggt
     2461 gagcaacccc agcgccatct tcctggccca gcacgccgaa ccggtgccgg gcagctgcgt
     2521 gctggtgacg ctggagggca cgcgcccgct gctggtggaa gtgcaggcgc tggtcgacag
     2581 cggcggaccc agcccgcggc ggctgtcggt ggggctggag cgcgaccgcc tggccatgct
     2641 gctggcggtg ctgcaccgcc acgccggcgt cgccacgggc gaccaggacg tgttcgtcaa
     2701 cgccgtcggg ggcgtgcgca tcagcgagcc ggcggccgac ctggcggtgc tgctggcgat
     2761 tgcctcgtcg ctgcgcggca agccgctgcc caagggcttc atcgcctttg gcgaggtcgg
     2821 cctggccggt gaggtgcgcc cggcgccgcg cggtcaggag cgactgaagg aagccgccaa
     2881 gctgggtttt tcggtggcgc tgatcccgcg cgccaacgcg cccagaaagg ccgacccggc
     2941 gttcgaaggc gtgacgctgc acaccgtcga gcgcgtggac gaggccatgg ccctgctgcg
     3001 caccctggag tgacgccgcg atgatggccg ccgcagccac cgacggttgg gaatgaaatt
     3061 gacctccagc gcttatgcag taagcgccgt cagctacatt aacgatagcg tttcgtgggg
     3121 tcgtggtggc gggggacaat agcgccatga agtggcaacg tctgtgggtg atgctgggcg
     3181 ccgtggcgct gggggccggc gcctggcgcg ccggtggctg ggccgggctg gcgctgctgg
     3241 gcagcgggct ggtgctgtgg ttcctgctgc actaccaccg cctgctgacg gtgatgaagc
     3301 gcgccaacga gcggccgatc ggccacatcg gcagcgccgt gatgctccac gccaagctgc
     3361 gtcagggcct gccgctgctg catgtcatgg cgctgaccca gtcgctgggc gagcgactgt
     3421 cggcgcccga ggcgcagccc gaggtgtatc gctggagcga cccgggccag tcgcatgtgg
     3481 tgaccgagtt cgaagctggc cgtctggtgc gctggcggct cgaacgcccg gccgatccgg
     3541 acgtccaggc cagcacgccc gaggcccatt gattcggcct ttggctgact ggaatgccgt
     3601 tcgggctgag cctgtcgaag ccctccgaca ggctcagggt gaacggttcg aagttcaaga
     3661 ttcgaagcaa tagaattcac ggtttgcgcg cacccgcccg caacccgcgc ccgttccgca
     3721 gaccccgagg acccccacca tgagcacccc cccgctttcc gaccgcgacg gcaagatctg
     3781 gctggacggc cagctggtcg actggcgcga tgccaagatc cacgtgctga cgcacaccct
     3841 gcactacggc tgcggcgcct tcgagggcgt gcgcgcctac gacaccgtgg gcggcaccgc
     3901 cgtcttccgc ctgcaggagc acacccagcg cctgttcaac agcgccaaga tcctgcgcat
     3961 ggccatcccg ttcacgcccg agcaggtcaa cgaagcgcag aaggaagtgg tgcgcgccaa
     4021 ccagctcaag agctgctacc tgcgcccgct ggtgtggatc ggctctgaaa agctgggcgt
     4081 cagccccaag ggcaaccgca tccacgtgat ggtggccgcc tgggcctggg gcgcctacct
     4141 gggcgaagaa ggcctcaagc gcggcatccg cgtcaagacc agcagctaca cgcgccacca
     4201 cgtcaacatc accatgacgc aggccaaggc cgtgagcaac tacagcaact cgatcctggc
     4261 caacatggaa gccaccgacg acggctacga cgaggcggtg ctgctcgaca gcgccggctt
     4321 cgtcagtgaa ggcgcgggcg agaacctgtt catcatcaag gacggcaccg tctacacgcc
     4381 cgacctgtcg gccggcgcgc tcaacggcat cacccgcaac acggtgctgc acatctgcga
     4441 ggacctcggg ctgaaggtgg tgcaaaagcg catcacgcgc gacgagtgct acatcgccga
     4501 cgagatgttc ttcaccggca ccgcggccga ggtgacgccg atccgcgaat acgaccgcgt
     4561 ccagatcggc gccggccagc gcggcccggt gaccgaaaag atccagagcg ccttcttcga
     4621 catcgtcaac ggccgcaacg ccaagtacgc ccactggctg gccaaggtct gagccgggtc
     4681 aacaaggagc atcgcgcatg agcacgccca accaagtcgt cgaactgctg gcccgcgacc
     4741 tgacccgctc gggcgagacc ttctgccccc atcccaaggc cgacatgaag ctctggaaca
     4801 cccacccgcg cgtgttcctc aacctggccc agggcgaagc ccgatgccct tactgcggca
     4861 ccctgtaccg catgaaggcc ggcgaggtgg tcggcgccgg ccactgacac tgccggcgcg
     4921 gcccggcact gcggccgcgt tgcaccagcg ctcagcggcc cgcctgcggc gccaccgcgc
     4981 ccgccggcca cagaccctgc caaagaggct gcggctgatg ggccagccgc tggatcacca
     5041 gcaccacgaa ccacaggctc aggcccatgg aggcgtcgcg cagcgccgag ttgcccagca
     5101 ccgccagcag catggccagc agcgccagcg ccgccaggct gccgttcaga tcgtcacgcc
     5161 gccaagccgc cggccagccg gccagcagca gccacagcag cagcagcgca cccggcaccc
     5221 cggtctgagc gcccatccac agaagatcgt tgtgcggcat gttgacgtcg gtgagcgggc
     5281 cgttcacccg ctgcctccac tgcgtgttcc agccgccaat gccccagccg agcaacggcc
     5341 gctcggcgat catggccgtg gtttcccggt acatgcggta gcggatgccc cagctgctgt
     5401 ccatctcgac cgtctgcccc tggtaggcac tgaccagttc ccccgcgccc tgctgcagcc
     5461 gctgcttgac gcccggcagc agcaggccgg ccgccagcgt cagcagcgcc gccgtcgcca
     5521 gcgccgccag ctgagggcgt cgccgaccgc gcaggcggtg ccacagcccc agggccagcc
     5581 cgagcaccag caccagccag gcggtgcgcg aatgcagcac ccagatcagc accagcatca
     5641 cggcggcggc cagcagtgcc gccagcacct gaccgcgccg gcccagggcc ggcagcagca
     5701 gcaggctgcc cgccgccacc atcgccatct gcactgcgtt ggccagcgac ttgttgccgt
     5761 cgtagcgcac caggctgtcc cacagcgggt ggggcggcaa cggcaccagc ccatggagtg
     5821 ccaccagcag caggccgacg gccagcccca gcagcacgcc gcgcagcgcc cacagcgcct
     5881 cgtcgcgcgt gagcgccagc gccagcgcca gcgtcagcac gatgcgcagc gcgtgcaggg
     5941 cgttggatgg ggtctccgca tagtgcggcc ccagcagcaa aatcagcgtg ctccagccca
     6001 cgagggccca ggccggccac cactggggcg cctgccgcag gcgcgccagg cgctcggccc
     6061 gatcgccctg ccacaccatg gccaccgcca gcaggacgaa ggcggtgtag ttcaggccga
     6121 tcgggaagaa ggcaacgaat ccccagaaca gcgcgaccca acgaaccatg gggagttgcg
     6181 gcatgggcgg caattgtagg tggcgccccg aacgaccgcg gtgtaagccg atgcaaaaaa
     6241 caaggccgcc tgatggcggc cttggggggt gacggagccc gggttcttga tttttttgcc
     6301 gggcctttgc gagagcggtt cacgcaaact cacccgtgtg acagcaatgt cgccaactgt
     6361 atacgcggat cgcccttcga ccagtccccc atttgcggga ttttgcgcaa ttaatcaaca
     6421 aaagaactat tttgtcgccg cgtccgcctc caccggcagc cgcagcgtga atcgcgcccc
     6481 gccgccgggt cggtggtcgc aggtgacgct gccgccgtgg cgctgggcga tggagcgcac
     6541 cagggccagc cccaggccca cgccgccttc gcgctcgctg gcgccgggca ggcggtagaa
     6601 gggctcgaag atgcgctcgc gctgctcggg cggcacgccg gggccgcgat cgtcgacctg
     6661 cagttcggcc gcgccaccag ccagttgcag gtgagcaccg acttcgccgt ctcgcccggt
     6721 gcggcggcca tggcgcacgg cgttttcgag caggttgcgg accgcgcggc gcagcagcct
     6781 ggcgacgccg ggcaccacca cctgccgggt gccatcggcc agggtgaatt cggcgccgac
     6841 gcgcgcgcat tcctcggcca gcaggccgat caggtcgacc ggctcgaccg tgcccaggtc
     6901 ggcctcacgc gcgtccagcc ggctggccag caggatttca tcgaccagct ggtccagctc
     6961 ggtgatgctg cgctcgattt cggcgcgcgc ggccggcgaa ccctgcgggc cggccagcgc
     7021 catggccatg cggatgcgcg ccagcggcga gcgcagctcg tgcgaggcgt tggccagcag
     7081 cgacttctgc gactgcagca gcgcgccctg ggcgtccatc agggtctgga tgcgctcggc
     7141 ggcggcgttg aagcggcgcg acaggtcggc cacctcgtcc tggccgcttt cgggcaggcg
     7201 cgccgacagg tcgccctcgc cccagcgctg cacgccgcgc tgcagcgcct ccagccgctg
     7261 ggtcaggcgg cgcgccaccg ggtacacccc cagcatcacg cccagaccgg ccagcgccag
     7321 cagccaccag aagccgtagg gcgggcgcag ccaggacggc ggcaggtcgg catggcggcg
     7381 ggcggcggcg cccgcggcag cgcggccgcg gccgcgcggc ggcatctgca ggctcagcac
     7441 ctggccgtcg cgcagggtga cgacgaattc gaagccctgc ccgggcagtc ggcgcggctg
     7501 cgcctcggcg gtgccgatca gttcgcccgc ggcgttgcgc accagcactt cgcgcggctg
     7561 gccggcctgg cggtcgcgct cgcgctcctg ctcggcggcg tgccagcccc agccgatcag
     7621 caggctgagc accagcacgc tgccggccac cgccagccag atgcgcaggt acagcctgcg
     7681 ggcaatggaa tcggtcactt tcacgccgac gaactatgaa atatgtagct gccggcgctt
     7741 gcctgacaag cgctggaggc cgattggatc gccatggcgg acggcacgct cagtcctgct
     7801 ggcgggcgaa cacgtagccg acgccgcgca cggtgaggat gcggcgcggg ttcttcacgt
     7861 cctgctcgat ggcggcgcgg atgcggccca tgtgcacgtc gatcgagcgg tcgaacgcct
     7921 ccagctcgcg cccgcgcacg gcttccatga tctgctcgcg cgtcagcacg cggccggcgc
     7981 gctcggccag ggtcaccagc aggtcgaact ggtagctggt gagttcggcc ggctggccgc
     8041 cgacgctgac ggtgcgggcg tcgcggtcga tctccagcgt gccgaagcgc agcagacggt
     8101 gttcgggctc ggcgcccggc tgctggcgcc gcagcacggc gcggatgcgg gccagcagct
     8161 cgcgcggctc gaacggcttg ggcaggtagt cgtcggcgcc cagctccagg ccgatgatgc
     8221 ggtccatcgg gtcgcccttg gcggtcagca tcagcaccgg cacgcgcgac gccccgtcgg
     8281 gcagggcgcg gatgcgccgg cagacctcca ggccatcgat gccgggcagc atcaggtcca
     8341 gcaccaccag gtcgaacggc tcgccctgca gggtctgcag tccggtttca ccgtcgccgc
     8401 ggtgcgtgac ggcgtagccc gattgcgtca ggtactcgcc caccatggcg gccaggcggg
     8461 cgtcgtcctc gatcatcagc aggcggtggg tactcatgcg ctcctttcac gctcggcgcg
     8521 gcgcacgcgc cacccgatgc tgcccgccat gatcgcggcg ccatgccagc gccgcttgtc
     8581 ggggcggtaa agaatcgtaa accgggcctg cggcggcgcc cggctgacta ttcaaacgat
     8641 agctgctcac gcttgccacc aaagcgctgg acgccgattc gatgccg
//
LOCUS       DAOQTZ010000105         8664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_9025_length_8664_cov_13.087350, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000105 DAOQTZ010000000
VERSION     DAOQTZ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9025_length_8664_cov_13.087350"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..890)
                     /locus_tag="PKN51_11180"
     CDS             complement(108..890)
                     /locus_tag="PKN51_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT85705.1"
                     /translation="MAKRSALPSGDQPLSASRTLIERAYEQLRDDIVDGHLPPDEKLR
                     VEHLKARYGVSAGTLREAITRLVSDALVITEGQRGFRVAPIAVADLQDLTNLRVHIEI
                     DALRQSMRQGGADWRQRVQAAYLDMSQLEQPLRPGHAKSWEALNIAFHEALLAGHDSP
                     WTLRLLRTLARQSERYRRYAISLPESRRDVHAEHREIYDSAMNGNELRAALALEAHIR
                     TTAELVARALHGNGHAPSTVRSTGPNGAGAVAPGSNGFAADR"
     gene            complement(910..2652)
                     /locus_tag="PKN51_11185"
     CDS             complement(910..2652)
                     /locus_tag="PKN51_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT85706.1"
                     /translation="MVFIDNRAVSELPPLPSTADLRRQVEFSSSDGRIWLAGQRMLLM
                     HAASLGTLRRELIQSIGPAAARRLLLRVGYASGERDAALARQLRADADMFSLFAVGPQ
                     LHMLEGAVQVTPEQLELDVAAGQFHGVFRWDHSWEVETHLRDFGPQHEPVCWTLLGYA
                     SGYTSAFMGRPVLYKEVACAACGHAHCRIEGRPVTDWPDGEQLASDYAAQSLTLDPPD
                     GAIGAWPAEVPADDQPETPVDALGPLIGQAPGFSAATQLLRKAAGTQVTVLLTGETGT
                     GKERFARALHALSPRAAKPFVAVNCAALPGELIESELFGTEKGAYTGADAARAGRFER
                     AHGGTLFLDELGELPLPAQAKLLRVLQDGQVERLGSSQPRHVDVRLVAATHVDLMQAV
                     RAGRFRQDLYYRINVYPIRIPPLREREGDIEPLARYLLQRFTLLHDKHVKGFADRALH
                     ALRTYHWPGNVRELENLIERGVILATAGQTMQVEHLFPEAGAPPGAAPTHTLAASGRL
                     REVPPPAAPDAHPWIDQLLTHQIGLTALEQALIEQAVQRSRGNLAAAARLLGLTRPQL
                     SYRLSKLRGERPST"
     gene            2879..3169
                     /locus_tag="PKN51_11190"
     CDS             2879..3169
                     /locus_tag="PKN51_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit"
                     /protein_id="HNT85707.1"
                     /translation="MADAPTPSDDRWDVQRRFIRIVEHHDNGMVEFEFAVGEPGLFVE
                     MVMPRAEFEEFCATQGVQPTEGRLPSRDGGSDAHEWDWSLRDARDRHFRHEP"
     gene            3211..4209
                     /locus_tag="PKN51_11195"
     CDS             3211..4209
                     /locus_tag="PKN51_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene monooxygenase hydroxylase
                     subunit beta"
                     /protein_id="HNT85708.1"
                     /translation="MQIDLRTVSITPLRQTYNHIAERLGADKPASRYIEGTMDVQANA
                     NFHYRPTWDPQHELFDPRRTRIVMQDWYALKDPRQLYYGTYTQARARMQETAEADFEF
                     VESRGLAERFDDAARRTALDFYVPLRHVAWGANMNGAYQCAYGYGTAITQPCLYAGMD
                     QLGIAQYLTRLGLLLGNQGDLEAGKHAWLNAPAWQGLRRLVEDTWVLKDWFELFVAQN
                     VVQDGVLFGLAYKEVDQAISEKAGPTVSMLTRFQAEWFQDASKWVDAVIKAAASESAD
                     NQALLSQWYGQWRARVQEALAPVAQLALGEQGAQALERAVAAVDARMVKAGLTLSA"
     gene            4244..4513
                     /locus_tag="PKN51_11200"
     CDS             4244..4513
                     /locus_tag="PKN51_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HNT85709.1"
                     /translation="MSNVFIAFQKNEDSRAIVNAIVADNPTAIVSDQPAMVKIDVPGR
                     MVIRKSTIEGEIGREFDLQEMHVHLITLSGNIDETDDEFSLTWNQ"
     gene            4548..6095
                     /locus_tag="PKN51_11205"
     CDS             4548..6095
                     /locus_tag="PKN51_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene/methane monooxygenase
                     hydroxylase/oxygenase subunit alpha"
                     /protein_id="HNT85710.1"
                     /translation="MDMKVNKKLGLKERYNLMTRDLAWTPTYQAVKDVFPYTEYEGIK
                     IHDWDKFEDPFRMTMDSYWKYQAEKERKLYAIIDAFTQNNGHLGVTDARYINTLKLFL
                     TGVSPLEYMAHRGFAHVGRQYPGAGPRVACLMQSLDEIRHAQTQIHSLSNYNKHYNGF
                     QNWRHQHDRVWYLSVPKSFFDDAVSAGPFEFIVAIGFAFEYVLTNLLFVPFISGAAYN
                     GDMGAMAFGFSAQSDESRHMTLGLEVIKFILEQDPDNLPIVQAWLDKWFWRGYRVLTL
                     VAMMMDYMLPKRVMSWKEAWEIYAEQNGTALFNDLARYGIKVPKGWEQACKDKDHLSH
                     QAWNVFYNYGAAAPFHTWAPSEKDMEWLSAKYPDTFDKYYRPVWEHYAKEQAEGRRFY
                     NGTLPMLCQVCQVPMFFTEPDDPTKIAYRESEYKGLKYHCCSDGCKHIFDNEPEKYAQ
                     SWLPVHQIYQGNCFPADVDPSKPDFNPLAEVLRWYKITQGQDNMDFADSPDKKNFEAW
                     RAQATSN"
     gene            6121..6477
                     /locus_tag="PKN51_11210"
     CDS             6121..6477
                     /locus_tag="PKN51_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit P4"
                     /protein_id="HNT85711.1"
                     /translation="MAVKSIAPYEFEQKDTVDKFPAPLLYIGWEDHKMFCAPFCAPMP
                     PTMKFGELVKGALPGMYGAHPDFARIDWAKAEWFMSGKPFTPDMDKTLAEHGIGHKSV
                     IRFRTPGLTGLSGSCF"
     gene            6591..7658
                     /locus_tag="PKN51_11215"
     CDS             6591..7658
                     /locus_tag="PKN51_11215"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenol 2-monooxygenase domain-containing
                     protein"
                     /protein_id="HNT85712.1"
                     /translation="MSYQLTIEPIGQVLEVEEGQTILDAALRAGIYLPHACGHGLCGT
                     CKIQVTEGEVEHGEASGFALMDYERDEGKCLACCARLESDLVIEADIEEDPDARNLLV
                     QDHQGVVSRIENLTPTIKGVWIELEAPITFQAGQYIGLHIPGETTTRAFSIASAPAQT
                     REIELNIRHVPGGKGTGWVHEQLRAGDRVSLAGPYGRFFVRESAHQKENLGYLFLAGG
                     SGLSSPRSMVLDLLAAGCNRPITLVNGARQRAELYHHDEFQQLAAQHPQFTYIAALSD
                     EPAGSDWDGARGFVHEAAKAHFNNDFRGHKAYLCGPPMMIDACITTLMQGRLFERDIY
                     TEKFISAADAQQVRSPLFKKI"
     gene            7669..8013
                     /locus_tag="PKN51_11220"
     CDS             7669..8013
                     /locus_tag="PKN51_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNT85713.1"
                     /translation="MAHHTITIEDTGESYRCADHRSVLEGMEALGKKGIPVGCRQGGC
                     GVCKVQILEGQYSKRVMSRAHVSAEEEASGCVLSCRIKPTSDLRLHVVGAMKKNVCRP
                     VSEAASVSTHPT"
     gene            8042..>8664
                     /locus_tag="PKN51_11225"
     CDS             8042..>8664
                     /locus_tag="PKN51_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT85714.1"
                     /translation="MTGVLRPGHAQIRVLDLEESVQWYTNVMGLQPMGRDAQGRAYFK
                     TRNERDHHSIILRQADQAGLDFFGYKVLDKATLDDLEKKLQAFGVKTERIPAGDLLET
                     GERVRFRIPTGHTLELYAEKKDVGNGMGYTDPEVDVLDGPGIRPIRMDHALIYGGDID
                     GNLKLFTEVLGFTLVERVKLEDGTTDLATWLTCSHKAHDIAMVRHAE"
BASE COUNT         1571 a         2921 c         2847 g         1325 t
ORIGIN      
        1 ttgcctggca cgccgatcac ggcacgggcg ggcgcgtcca actgccgttt ctaggatgac
       61 gaatggcgaa tgacctcgct gcgcttcgat gacgcgaggg gcaggggtca gcggtcggcg
      121 gcgaaaccgt tggagcctgg cgcgacggcg ccggcgccgt tcgggccagt tgatcggacc
      181 gtcgatggcg cgtggccgtt gccatgcagc gcccgcgcca ccagctcggc cgtggtgcgg
      241 atgtgcgctt ccagcgccag tgcggcgcgc agctcgttgc cgttcatcgc gctgtcgtag
      301 atctcgcggt gctcggcatg cacgtcacgt cggctctccg gcaggctgat ggcgtagcgg
      361 cggtagcgtt cgctctgccg tgccagggtg cgcagcaggc gcagcgtcca gggcgaatcg
      421 tggccggcca gcagcgcctc gtgaaaggcg atgttcagcg cttcccatga cttggcgtgg
      481 ccggggcgca gcggttgctc gagctgggac atgtccaggt acgccgcctg cacccgctgg
      541 cgccagtcgg cgccgccttg ccgcatcgac tggcgcaggg cgtcgatctc gatgtgcacg
      601 cgcaggttgg tcagatcctg cagatcggcg acggcgatcg gggccacgcg aaaaccgcgc
      661 tggccctcgg tgatcaccag cgcatcgctg accagccggg tgatggcctc gcgcagtgtg
      721 ccggcgctca cgccatagcg cgccttcagg tgttcgacgc gcagcttctc gtcgggcggc
      781 aggtgaccgt ccacgatgtc gtcgcgcagt tgctcgtagg cgcgctcgat cagcgtgcgg
      841 gacgccgaca ggggctggtc gccagagggc agggcagagc gtttggccat cgagtcccca
      901 ctgccggggt caggtggaag gccgttcgcc gcgcagcttg ctcagtcggt agctgagctg
      961 cggccgggtc agccccagca ggcgcgcggc cgccgccagg ttgccgcggc tgcgctgcac
     1021 ggcctgctcg atcagtgcct gctccagtgc cgtcaggccg atctggtgcg tcagcagctg
     1081 atcgatccat gggtgggcgt cgggcgcggc gggcggaggc acttcgcgca ggcgcccgga
     1141 ggctgcgagc gtgtgggtcg gagcggcgcc gggcggtgcg ccggcctcgg ggaacaggtg
     1201 ttcgacctgc atggtctggc cggccgtggc caggatcacg ccgcgttcga tcaggttttc
     1261 cagctcgcgc acgttgccgg gccagtggta ggtgcgcagt gcgtgcaggg cgcggtcggc
     1321 gaaccccttg acatgcttgt cgtgcaggag cgtgaagcgc tgcagcaggt agcgcgcgag
     1381 cggttcgatg tcgccctcgc gctcgcgcag cggcggtatg cggatcgggt agacgttgat
     1441 gcggtagtag agatcctgcc ggaagcgccc ggcacgcacc gcctgcatca ggtccacgtg
     1501 ggtggccgcg accagccgca cgtccacatg ccgcggctgc gacgagccca gacgttcgac
     1561 ctgcccgtcc tgcagcacgc gcagcagctt ggcctgggcc ggcagcggca actcgcccag
     1621 ctcatcgagg aatagggtgc cgccgtgggc gcgctcgaag cggccggcgc gggcggcgtc
     1681 ggcgccggtg taggcgcctt tctcggtgcc gaacagctcc gactcgatca gctcgcccgg
     1741 cagcgccgcg cagttgacgg cgacaaacgg cttggccgcg cgcgggctga gcgcatgcag
     1801 ggcgcgcgca aagcgctcct tgccggtgcc ggtctcgccc gtcagcagca ccgtcacctg
     1861 ggtgcccgcc gccttgcgca gcaactgcgt ggcggcgcta aaaccgggcg cctggccgat
     1921 cagcgggccc agggcgtcca ccggggtttc gggctggtcg tcggccggca cttcggcggg
     1981 ccaggcgccg atggcgccgt ccggcggatc gagcgtgagc gactgcgcgg cgtagtcgct
     2041 ggccagctgc tcgccgtcgg gccagtcggt caccggccga ccctcgatgc ggcagtgcgc
     2101 gtggccgcag gcggcgcagg ccacttcctt gtacagcacc gggcggccca tgaaggcgct
     2161 ggtgtagccc gaggcgtagc ccagcagggt ccagcagacg ggctcgtgct gggggccgaa
     2221 gtcgcgcaag tgggtttcca cctcccacga atggtcccag cggaacacgc cgtggaactg
     2281 gccggccgcc acgtccagct ccagctgttc cggcgtgacc tggaccgcgc cctccagcat
     2341 gtgcagctga gggccgacgg caaacagtga aaacatgtcg gcgtcggcgc gcagctggcg
     2401 cgccagcgcc gcgtcgcgct cgcccgaggc gtaaccgacg cgcagcagca ggcgccgcgc
     2461 ggcggccggg ccgatcgact gaatcagctc gcgccgcaag gtccccagcg aggccgcatg
     2521 catcagcagc atgcgctggc cggccagcca gatccggcca tcggacgacg agaactccac
     2581 ctggcggcgc agatcggcgg tggagggcag gggtggcagc tcggacacgg ctcgattatc
     2641 gataaagacc atctttgtgc aagatggcgg tcggatgaaa agtgatgaaa tcgtgaatcg
     2701 gccagttgat gaatgcttga tttcgtcatg ctgcagcgca atacttcacg ctttcgaccc
     2761 gcgtgaaggg gtggcgctcg gtgtcggtgg atggttctgt ttaggggttt tccctggaat
     2821 tatcgagatt tttcctgatt gcctacggtt tggcatgcac cgtgcaaggg atggcggcat
     2881 ggccgatgca cccaccccat ccgacgaccg ctgggacgtc cagcgccgct tcatccgcat
     2941 cgtggagcac cacgacaacg gcatggtcga gttcgagttc gcggtcggcg aacccgggct
     3001 gttcgtcgaa atggtgatgc cgcgcgccga gttcgaggag ttctgtgcca cccagggggt
     3061 gcagcccacc gaaggtcgtt tgccttcacg cgacggcggc agcgacgccc acgagtggga
     3121 ctggagcctg cgcgatgccc gcgaccggca cttccgccac gagccctgat ttccatccgt
     3181 tcaaaccacc aggccaagga gacacgtccc atgcagatcg acctgcgcac cgtcagcatc
     3241 accccgctgc ggcagaccta caaccacatc gccgagcggc tgggtgccga caagccggcc
     3301 tcccgctaca tcgaaggcac gatggacgtg caggccaacg ccaacttcca ctaccgcccc
     3361 acctgggatc cgcagcatga gctgttcgac cctcggcgta cccggatcgt gatgcaggac
     3421 tggtatgcgc tgaaggaccc gcgccagctg tactacggca cctacaccca ggcgcgcgcc
     3481 cgcatgcagg aaaccgccga ggcggatttc gagttcgtcg aaagccgcgg cctggccgag
     3541 cgcttcgacg acgccgcccg ccgcaccgca ctggacttct atgtgccgtt gcgccacgtc
     3601 gcctggggcg ccaacatgaa cggtgcctac cagtgcgcct acggctacgg cacggccatc
     3661 acccagccct gcctgtacgc cggcatggat cagctgggca tcgcccagta cctgacccgc
     3721 ctgggtctgc tgctgggcaa ccagggcgac ctggaagccg gcaagcacgc ctggctgaat
     3781 gcgcctgcct ggcagggtct gcgccgcctg gtcgaggaca cctgggtgct caaggactgg
     3841 ttcgagctgt tcgtggcgca gaacgtggtg caagacggcg tgctgttcgg tctggcctac
     3901 aaggaggtgg accaggcgat ctcggaaaag gccggtccca ccgtatccat gctcacgcgc
     3961 tttcaggccg agtggttcca ggacgccagc aagtgggtcg acgccgtcat caaggccgcc
     4021 gccagcgaaa gtgccgacaa ccaggcgctg ctcagccagt ggtacggcca gtggcgcgcc
     4081 cgcgtgcagg aggcgctggc cccggtggcg cagctggcgc tgggcgagca gggtgcccag
     4141 gcgctggaac gcgccgtggc cgccgtcgac gcccgcatgg tcaaggccgg cctgacgctg
     4201 agcgcctgac caccaccccc gaacacagca caggagtcaa ggcatgtcca acgtattcat
     4261 cgcctttcag aagaacgagg attcgcgcgc catcgtcaac gccatcgtgg ccgacaaccc
     4321 cacggccatc gtcagcgacc agccggccat ggtcaagatc gacgtgcccg gccgcatggt
     4381 gattcgcaag tccaccatcg aaggcgagat cggccgcgag ttcgacctgc aggaaatgca
     4441 cgttcacctc atcactctga gcggcaacat cgacgagacc gatgacgagt tcagcctgac
     4501 ctggaaccag taagcaccgc cccgaacgcg acgcaaggag acgcaccatg gacatgaaag
     4561 tcaacaagaa gctgggcctg aaagagcgct acaacctgat gacgcgcgat ctggcctgga
     4621 cacccaccta ccaggccgtc aaggacgtgt tcccctacac cgagtacgag ggcatcaaga
     4681 tccacgactg ggacaagttc gaagacccgt tccgcatgac gatggactcg tactggaagt
     4741 accaggccga gaaagagcgc aagctgtacg ccatcatcga cgccttcacg cagaacaacg
     4801 gccatctggg tgtgaccgat gcgcgctaca tcaacaccct caagctgttc ctgaccggcg
     4861 tctcgccgct ggaatacatg gcgcaccgcg ggtttgccca cgtgggccgc cagtaccccg
     4921 gcgccggccc gcgcgtggcc tgcctgatgc agagcctgga cgaaatccgc cacgcgcaga
     4981 cccagatcca cagcctgtcc aactacaaca agcactacaa cggcttccag aactggcgcc
     5041 accagcacga ccgcgtgtgg tacctgtcgg tgcccaagag tttcttcgac gacgccgtga
     5101 gcgccgggcc gttcgagttc atcgtggcca tcggctttgc cttcgaatac gtgctcacca
     5161 acctgctgtt cgtgcccttc atctcgggcg cggcctacaa cggcgacatg ggcgccatgg
     5221 cgttcggctt ctcggcccag tccgacgagt cgcgccacat gacgctgggt ctggaagtga
     5281 tcaagttcat cctggaacaa gacccggaca acctgccgat cgtgcaggcc tggctggaca
     5341 aatggttctg gcgcggctac cgcgtgctga ccctggtggc catgatgatg gactacatgc
     5401 tgcccaagcg cgtgatgagc tggaaggaag cctgggaaat ctacgccgag cagaacggca
     5461 cggccctgtt caacgacctg gcgcgctacg gcatcaaggt gcccaagggg tgggagcagg
     5521 cctgcaagga caaggaccac ctgtcgcacc aggcctggaa cgtgttctac aactacggcg
     5581 ccgccgcccc cttccacacc tgggcgccga gcgaaaagga catggagtgg ctgtcggcca
     5641 agtaccccga caccttcgac aagtactacc gccccgtgtg ggagcactac gccaaggagc
     5701 aggccgaggg ccggcgcttc tacaacggca cgctgcccat gctctgccag gtgtgccagg
     5761 tgccgatgtt cttcaccgag ccggacgacc ccaccaagat tgcctaccgc gagagtgaat
     5821 acaaggggct caagtaccac tgctgctccg acggctgcaa gcacatcttc gacaacgagc
     5881 ccgagaagta cgcccagagc tggctgcccg tgcaccagat ctaccagggc aactgcttcc
     5941 ccgcggacgt ggacccgagc aagcccgatt tcaacccgct ggccgaggtg ctgcgctggt
     6001 acaagatcac ccagggccag gacaacatgg acttcgccga ctcgcccgac aagaagaact
     6061 tcgaagcctg gcgcgcgcag gccaccagca actgaccggg aaccctcagg agacttaccc
     6121 atggctgtca aatccatcgc cccctacgag ttcgagcaga aagacacggt ggacaaattt
     6181 cccgcaccgc tgctgtacat cggctgggag gaccacaaga tgttctgcgc gccgttctgc
     6241 gcccccatgc cgccgaccat gaagtttggc gagctcgtca agggcgcgct gcccggcatg
     6301 tatggcgcgc acccggactt tgcgcgcatc gactgggcca aggccgaatg gttcatgtcc
     6361 ggcaagcctt tcacgcccga catggacaag accctggccg agcacggcat cggccacaag
     6421 agcgtgatcc gctttcgcac gccggggctg accgggctga gcggcagctg cttctgaggc
     6481 gaatggccgc cggccgccga caaagcacct gccgaatatg gatcaaatcg gcctccagcg
     6541 cttgacagac aagcgccggc agctattagt tttgaagttt ccgtacaccc atgagctacc
     6601 agctcacgat tgaaccgatc ggccaggtcc tcgaggtcga ggagggccag accattctgg
     6661 acgccgccct gcgcgccggc atctacctgc cgcacgcctg cggccatggc ctgtgcggca
     6721 cctgcaagat ccaggtgacc gaaggcgaag tcgagcacgg cgaagccagc ggctttgccc
     6781 tgatggacta cgagcgcgac gaaggcaagt gcctggcctg ctgcgcccgg ctcgaatccg
     6841 acctcgtcat cgaggccgac atcgaggaag acccggacgc ccgcaacctg ctggtgcagg
     6901 accatcaggg ggtggtctcc cgcatcgaga acctgacgcc caccatcaag ggcgtgtgga
     6961 tcgagctcga agcgcccatc accttccagg ccggtcagta catcgggctg cacattccgg
     7021 gcgaaaccac cacccgcgcg ttctcgatcg ccagcgcgcc ggcgcagacg cgcgagatcg
     7081 agctgaacat ccgccatgta cccggcggca agggcaccgg atgggtgcac gagcagttgc
     7141 gggcgggcga ccgcgtcagc ctggccgggc cctacgggcg cttcttcgtg cgcgagtccg
     7201 cgcaccagaa ggagaacctg ggctacctgt tcctggccgg cggctcgggc ctgtccagcc
     7261 cgcgctccat ggtgctcgat ctgctggcgg ccgggtgcaa caggccgatc accctggtca
     7321 acggcgcgcg ccagcgtgcc gagctctatc accacgacga gtttcagcaa ctggcggcgc
     7381 agcacccgca attcacctac atcgcggcgc tgtcggacga acccgcgggc agcgactggg
     7441 acggcgcgcg cggcttcgtg cacgaggccg ccaaggcgca tttcaacaac gacttccgcg
     7501 gccacaaggc ctacctgtgc ggtccgccga tgatgatcga cgcctgcatc accaccctga
     7561 tgcagggccg gctgttcgag cgcgacatct ataccgagaa attcatctcg gccgccgatg
     7621 cgcagcaggt gcgcagtccg ctgttcaaga agatctgagg cggcgaccgt ggcgcaccac
     7681 accatcacca tcgaggacac cggcgaaagc taccgctgcg ccgaccaccg ctccgtgctg
     7741 gaaggcatgg aagccctggg caagaagggc atccccgtgg gctgccgtca gggcggctgc
     7801 ggcgtgtgca aggtgcagat cctggaaggt cagtacagca agcgcgtgat gagccgcgcc
     7861 catgtcagcg ccgaagaaga ggccagcggc tgtgtgctgt cgtgccgcat caagcccacc
     7921 agcgacctgc gcctgcacgt cgtcggcgcc atgaagaaga atgtctgccg ccccgtcagc
     7981 gaggcggcca gcgtatccac ccaccccacc tgaaaccaag cgaaggagac ctactatggc
     8041 aatgactggc gttctgcgcc ctgggcacgc ccagatccgc gtactcgacc tggaagaatc
     8101 ggtccagtgg tacaccaacg tgatgggcct gcagcccatg ggccgcgatg cccagggccg
     8161 cgcctatttc aagacccgca acgagcgcga ccaccacagc atcatcctgc gccaggccga
     8221 ccaggccggg ctggacttct ttggctacaa ggtgctcgac aaagccacgc tcgatgacct
     8281 ggagaaaaag ctccaggcct ttggcgtcaa gaccgaacgc atccccgccg gtgacctgct
     8341 ggagaccggc gagcgcgtgc gcttcaggat tcccaccggc cacacgctgg agctgtacgc
     8401 cgagaagaag gacgtgggca acggcatggg ctacaccgac cccgaggtgg acgtgctcga
     8461 cggccccggc atccgcccga tccgcatgga ccatgcgctg atctacggcg gcgacatcga
     8521 cggcaacctg aagctgttca ccgaggtgct gggcttcacc ctggtcgagc gcgtcaagct
     8581 ggaagacggc accaccgacc tggccacctg gctgacctgc agccacaagg cgcacgacat
     8641 cgccatggtg cgccacgccg agga
//
LOCUS       DAOQTZ010000106         8652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_9044_length_8652_cov_13.767012, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000106 DAOQTZ010000000
VERSION     DAOQTZ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9044_length_8652_cov_13.767012"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1231)
                     /locus_tag="PKN51_11230"
     CDS             complement(134..1231)
                     /locus_tag="PKN51_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85715.1"
                     /translation="MFLRIDQVEVRYPGAAQPAVQGATLGLRAGDIGVLIGPSGCGKT
                     TLLRAVAGLEPVSAGTIHLGDTLVSSARVHLAPEQRRIGMVFQDYALFPHLDVAHNVA
                     FGIRHLPAAARAARVAEVLGLVDLAGSQGRYPHELSGGQQQRVALARAMAPAPGLLLL
                     DEPFSNLDVDLRERLAHELRGILKAAGATALFVTHDQLEAFAIGDQIGVMHQGRLHQW
                     DDAYSLYHRPATRFVADFIGHGVFVPAQIVAHEGDGAQLDTPLGPLRHLTDCPLPGAY
                     PQGRCELLLRADDIVHDDAAPVQAQIVRKAFRGSEFLYTLRLVSGETVLAHVPSHHNH
                     RVGEWIGIRPEVDHVVTFPVGADHPVSPGAG"
     gene            1440..3089
                     /locus_tag="PKN51_11235"
     CDS             1440..3089
                     /locus_tag="PKN51_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNT85716.1"
                     /translation="MLAFLLRPVPTLLLVLLAGALALPVLVVLGSWVQWDAQTAQILR
                     EMAATVLPEYLWVTVQLALMVGLGVVAMGVPAALLVTVFDFPGRRSFEWLLLLPLAMP
                     AYVTAYAYTDFLQFSGPLQSWLRASFGLQGRLLPEVRSVWGAAWVFSFSLYPYVYLLA
                     RTALVERAPQLMEAARLLGASLPRRLREVALPMARPAVAAGTALALMEVLADYGVSVY
                     FGIQTFTAGIYKAWLSMDNRIAAAQLATVLLALVVLLLWLEQRAQRRLRFASASAARA
                     GSGEARPVRLRGRRAALAWLLCLLPVLLGFVLPVLFMLRPLSADWSVLPWERFLVWAW
                     NSVRLGGMTALLALGAAWLLAFGLRRRPSLLRRGAVQLISLGYAVPGAVVVVGLLLPV
                     GWLQARWPGSGAGALVTASVLGLLWAYLVRFVSVALQSVQSGYARVSHSLDESARLLG
                     ASGLELALQVHWPLLRRAGLAAALLVFVDVMKELPATIVLRPFNTDTLAVVANQLARD
                     ERLGEAALPSLALVLVGLVPVVLLSRTLRAERRAQGDAARV"
     gene            3120..3461
                     /locus_tag="PKN51_11240"
     CDS             3120..3461
                     /locus_tag="PKN51_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT85717.1"
                     /translation="MDPTDPGRPDAALEADQVFELAAEVFRVMSAPMRLKIISALCNG
                     EKNVGQLLAEIRTTQPNMSQHLNTLYQAGVVGKRRDGVQIYYRIINDRVVNLCRAVCT
                     QIAMETDGDPA"
     gene            3607..3918
                     /locus_tag="PKN51_11245"
     CDS             3607..3918
                     /locus_tag="PKN51_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I cytochrome c"
                     /protein_id="HNT85718.1"
                     /translation="MKPILLSGFAAAALALVASAAQAQTDPLQVRSWAAACANCHGTN
                     GHAQTGNEPLAGGNKDEMLKKLLDFKRGAKPATIMHQLSKGYSDEQLAAIAAWFAAQK
                     K"
     gene            3930..5204
                     /locus_tag="PKN51_11250"
     CDS             3930..5204
                     /locus_tag="PKN51_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT85719.1"
                     /translation="MDRRQFIQAGSAASVLGLTAGCATTGGGAGGKVVVIGGGYGGAT
                     AAKYIRMWSEGRVEVTLVEPGESFISCPISNLVLGGSKQMADITTPYDNLSRRHGVKM
                     VRDRVSAIDADKRQVRLASGGVLPYDRLVVSPGVDFMWDALPGLKKPGAQDKVLHAWK
                     AGPQTVALRKQLEAMPDGGVYALSIPLAPYRCPPGPYERACQVAHYFSKAKPKSKVLI
                     LDSNDDVTSKGPLFKKAWADRYKGIIEYRPKHALADVDAATGTLKFEFNDDIKAQVLN
                     VIPPMRAGDVAHHAGLTTANKRWCDVDWLTMESKAAKNVHILGDALQIAPAMPKSGHM
                     ANQHGKVAAAAIVALLSGRQPNPLPIYNNTCYSFVSDTDVVHVASVHAYDADKKTMVP
                     VAGSGGVSSAASELEGRYAMAWARNIWADSLA"
     gene            5342..5917
                     /locus_tag="PKN51_11255"
     CDS             5342..5917
                     /locus_tag="PKN51_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNT85720.1"
                     /translation="MLAALALAPALAQDSGEARARKIIGGSCFLCHGAEGESASEVFP
                     RLAGQNAEYIAKQLENFKSGKRKSTAMASMVTELTLDDMVALGRFYAARPPHKEAAQD
                     AALAGVGRYIYQSGNKWSGVPSCASCHGAEGAGSTTLPRLAGQHAAYLETQLKQFSKR
                     ERNNDNAVMHTVVEKMTGLEMAAVSEYLSGK"
     gene            complement(6020..7129)
                     /locus_tag="PKN51_11260"
     CDS             complement(6020..7129)
                     /locus_tag="PKN51_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNT85721.1"
                     /translation="MAIDLTSLVEQHGSGTVLAVGGALIGMLFGFFAQRSRFCLRAAV
                     IEFWRGQFAEKLSVWLLAFAAAVMAVQALILAGALNVSEARQLANRGSLSGALVGGLV
                     FGAGMIMTRGCASRLLVLSANGNLRALLSGLVFAVTAQAALAGALAPLRSEISSWWTV
                     DGGAARDLLARVGAGHADGLAFGGLWLVVAIVFSLRSGNRPWMWIGGIGSGLAVALAW
                     WFTYAVAHASFDPVQMQGLTFSGPSAEWLMRVLASPAPAWGFDAGMLPGVFVGSALGA
                     LVGRDFKVEGFKDGYSMARYIAGAVLMGFGSMLAGGCAVGAGMTGGAIFALTAWLTLA
                     GMWAGGGLMHRLLDEPVQTSQPASQQAPVVLPVAP"
     gene            7175..8161
                     /locus_tag="PKN51_11265"
     CDS             7175..8161
                     /locus_tag="PKN51_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT85722.1"
                     /translation="MRPVIDRRQLLQRCACLALGGMGAAWARYAEAADTPASAPAMQA
                     REAAPGCWYVEGLSALGSSANQNFISNAGFVVTSSGVVVIDALGSPALAERLLAEIRK
                     VTAQKVTHVIVTHYHADHIYGLQVFEALGARILAHQAAREYLNSDTARLRLQASREEL
                     APWVDARTRLVPATEWIHERRELTVGDTTLVLQPVGPAHTPEDLAVYLPGVKALYAGD
                     LVFRSRIPFVGQADSRNWIVSLDRLLAFDTRVIIPGHGPVSTEARQDMQLTRDYLVYL
                     RKTMGAAARDMTPFDEAYRATDWSMYEHLPLFGPANRMNAYNTYLQMEHEPQ"
     gene            8158..8652
                     /locus_tag="PKN51_11270"
     CDS             8158..8652
                     /locus_tag="PKN51_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85723.1"
                     /translation="MNTPSRFSPARRHGLQALALLGLGAGWAPPAQAAGKTLPLSASL
                     ADELARALKARQPLVVMVSLHRCPWCEEVRNNYLAPMRAQDGLPVVQLDMLSPRATRD
                     LQGAATTHDALVRAWDVKVAPTVLFLGPRGKEVADRLVGGSPDFYSAYLDRRLALARE
                     AVVA"
BASE COUNT         1284 a         3111 c         3002 g         1255 t
ORIGIN      
        1 gcacccaccc gcaccgcgct cggcgcgcca tgcggcgttg ctcctgcttg cgggctcgag
       61 cccgcggcat cgtcgcgtct tgcctggcac gccgatcacg gcacgggcgg gggcgtccga
      121 ccgcctcctg ggctcagccg gcgccggggc tgaccgggtg gtcggcgccg accgggaagg
      181 tcaccacgtg gtccacctcg ggccggatgc cgatccattc gccgacgcgg tggttgtggt
      241 ggctgggcac atgcgccagc accgtctcgc cgctgaccag ccgcagggtg tagaggaact
      301 ccgagccgcg aaaggccttg cgcacgatct gggcctgcac cggcgcggcg tcgtcgtgca
      361 cgatgtcgtc ggcgcgcagc agcagctcgc agcggccctg cgggtaggcg ccgggcagcg
      421 ggcagtcggt caggtggcgc agcgggccca gcggggtgtc cagttgcgca ccgtcgcctt
      481 cgtgggcgac gatctgcgcc ggcacgaaca cgccgtggcc gatgaagtcg gccacgaagc
      541 gcgtggccgg ccggtggtag aggctgtagg cgtcgtccca ctggtgcagc cggccctggt
      601 gcatgacgcc gatctggtcg ccgatggcga acgcctccag ctggtcgtgg gtgacgaaca
      661 gggcggtcgc cccggccgcc ttcaggatgc cgcgcagctc gtgcgccagg cgctcgcgca
      721 ggtccacatc gaggttggaa aacggctcgt ccagcagcag caggcccggc gccggcgcca
      781 tggcgcgcgc cagcgccacg cgctgctgct gtccgcccga cagctcgtgg gggtagcggc
      841 cctggctgcc ggccagatcg accaggccca gcacctcggc cacgcgcgcc gcacgcgcgg
      901 cggccggcag gtggcggatg ccgaaggcca cgttgtgcgc cacgtccagg tgcggaaaca
      961 gcgcgtagtc ctgaaacacc atgccgatgc ggcgctgctc gggcgccagg tgcacgcggg
     1021 cgctgctgac cagcgtgtcg cccagatgga tggtgccggc gctgaccggc tccagccccg
     1081 ccaccgcgcg cagcagcgtg gtcttgccgc agcccgaggg gccgatcagc accccgatgt
     1141 cgcccgcgcg cagccccagc gtggcgccct gcacggcggg ttgtgcggcg ccgggatagc
     1201 gcacttcgac ctggtcgatg cgtaggaaca tggtgggcag cgattgtaga tacgtacaat
     1261 tctcattcag ggaggcgagg gccgccgcac cccatgccgc agccgcggcg cgcggcaggc
     1321 cgccgcgggg gcgtcccatc gcccgccagc ggccacgccg cgcgttcggg gtcagaatgg
     1381 cctctggcgc gcacctgacc tgcgccagca gctatacatt tgataacaat ctgaccatcg
     1441 tgctggcatt ccttttgcgc cccgtcccca ccctgctgct ggtgctgctg gcgggcgcgc
     1501 tggcgctgcc ggtgctggtg gttctgggct catgggtgca gtgggacgcc cagacggcgc
     1561 agatcctgcg cgagatggcc gccaccgtgc tgcccgaata cctgtgggtg accgtgcagc
     1621 tggcgctgat ggtgggcctc ggcgtggtgg ccatgggcgt gccggcggcg ctgctggtga
     1681 ccgtgttcga cttccccggc cggcgcagtt tcgaatggct gctgctgctg ccgctggcca
     1741 tgccggccta tgtcacggcc tatgcctaca ccgacttcct gcagttttcc ggcccgctgc
     1801 aaagctggct gcgcgccagc ttcggcctgc aggggcggtt gctgcccgag gtgcgcagcg
     1861 tgtggggcgc ggcctgggtg ttttcgttct cgctctatcc ctacgtgtac ctgctggcgc
     1921 gcaccgcgct ggtcgagcgg gcgccgcagc tgatggaagc ggcgcgcctg ctgggcgcca
     1981 gcctgccgcg ccggctgcgc gaagtggcgc tgccgatggc gcgcccggcg gtggccgccg
     2041 gcaccgcgct ggccctgatg gaagtgctgg ccgactacgg cgtgtcggtg tacttcggca
     2101 ttcagacctt caccgccggc atctacaagg cctggctgtc gatggacaac cgcatcgccg
     2161 ccgcccagct ggccacggtg ctgctggcgc tggtggtgct gctgctgtgg ctggagcagc
     2221 gcgcccagcg ccggctgcgc tttgccagcg ccagcgcggc gcgcgccggc tccggcgagg
     2281 cgcggccggt gcgcctgcgc ggccggcgtg ccgcgctggc ctggttgctg tgcctgctgc
     2341 cggtgctgct cggcttcgtg ctgccggtgc tgttcatgct gcgtccgctg tcggccgact
     2401 ggtcggtgct gccctgggag cgctttctgg tctgggcctg gaacagcgtg cgcctgggcg
     2461 gcatgacggc gctgctggca ctgggcgcgg cctggctgct ggccttcggc ctgcgccgcc
     2521 ggccgagcct gctgcggcgc ggcgcggtgc agctcatctc gctcggctac gcggtgccgg
     2581 gcgcggtggt ggtggtgggt ctgctgctgc cggtgggctg gctgcaggcg cgctggccgg
     2641 gcagcggcgc cggcgccctg gtcaccgcca gcgtgctggg cctgctgtgg gcctacctgg
     2701 tgcgcttcgt gtcggtggcg ctgcagtcgg tgcaaagcgg ctatgcgcgg gtgtcgcaca
     2761 gcctggacga atcggcgcgc ctgctgggcg ccagcgggct ggagctggcg ctgcaggtgc
     2821 actggccgct gctgcgccgc gccggcctgg cggcggcgct gctggtgttc gtcgacgtca
     2881 tgaaggagct gcccgccacc atcgtgctgc gccccttcaa caccgacacc ctggcggtgg
     2941 tggccaacca actggcgcgc gacgagcgcc tgggcgaggc ggccctgccg tcgctggcgc
     3001 tggtgctggt ggggctggtg ccggtggtgt tgctgagccg caccctgcgc gccgagcggc
     3061 gtgcgcaggg cgacgccgcg cgggtatagt cacccggcgc cgtgcacagc gaacccgcca
     3121 tggaccccac cgaccccggc cgacccgacg ccgccctcga agccgaccag gtgttcgagc
     3181 tggcggccga ggtgtttcgc gtcatgtcgg caccgatgcg gctgaagatc atcagcgcgc
     3241 tgtgcaacgg cgaaaaaaac gtcggccagc tgctggccga gatccgcacc acccagccca
     3301 acatgagcca gcacctcaac accctctacc aggccggtgt ggtgggcaag cggcgcgatg
     3361 gggtgcagat ctactaccgc atcatcaacg accgcgtggt caacctgtgc cgcgccgtct
     3421 gcacccagat cgccatggaa accgacggcg acccggcctg acgcgcggca ccgggccgtg
     3481 aagctgcctt cggggttaat cctgaccaag gtcatgcttc gagcgcccta tcatgcggcg
     3541 accatataag cggttgcgaa ttttcccttc tgcccccggc agtcccaacc aggaggaccc
     3601 acgaccatga aacccatttt gctttccggc ttcgccgcgg cggccctggc gctggtcgcc
     3661 agcgccgcgc aggcgcagac cgatccgctg caggtgcgca gctgggccgc cgcctgcgcc
     3721 aactgccacg gcaccaacgg ccatgcccag accggcaacg agccgctggc cggcggcaac
     3781 aaggacgaga tgctgaagaa gctgctcgac ttcaagcgcg gcgccaagcc cgccaccatc
     3841 atgcaccagc tcagcaaggg ctacagcgac gagcagctgg ccgccattgc ggcctggttc
     3901 gcggcgcaga agaaatgagg aggcacacca tggatcgtcg tcagttcatt caggccggtt
     3961 cggccgcttc cgtgctgggc ctgaccgcgg gctgtgccac caccggcggc ggtgccggtg
     4021 gcaaggtggt ggtcatcggt ggcggctacg gtggcgccac cgcggccaag tacatccgca
     4081 tgtggtccga gggccgtgtc gaggtcacgc tggtcgagcc gggtgagagc ttcatctcct
     4141 gcccgatctc caacctggtg ctgggcggca gcaagcagat ggccgacatc accaccccct
     4201 acgacaacct gagccgccgc catggcgtga agatggtgcg cgaccgggtc agcgccatcg
     4261 acgccgacaa gcgccaggtg cggctggcca gcggcggcgt gctgccctat gaccggctgg
     4321 tggtgtcgcc cggtgtcgat ttcatgtggg atgccctgcc gggattgaag aagcccggcg
     4381 cccaggacaa ggtgctgcac gcctggaagg ccggcccgca gacggtggcg ctgcgcaagc
     4441 agctcgaagc catgcccgac ggcggcgtgt acgccctcag catcccgctg gcgccctacc
     4501 gctgcccgcc cggcccgtat gagcgcgcct gccaggtggc gcattacttc agcaaggcca
     4561 agcccaagtc caaggtgctg attctcgact ccaacgacga cgttacctcc aaggggccgc
     4621 tgttcaagaa ggcctgggcc gaccgctaca agggcatcat cgaataccgg cccaagcacg
     4681 cgctggccga cgtcgacgcc gccaccggca cgctcaagtt cgagttcaac gacgacatca
     4741 aggcccaggt gctgaacgtc atcccgccca tgcgcgccgg cgacgtggcg caccacgccg
     4801 ggctgaccac cgccaacaag cgctggtgcg acgtcgactg gctgaccatg gaatccaagg
     4861 ccgccaagaa cgtccacatc ctgggcgacg cgctgcagat cgccccggcc atgcccaaat
     4921 ccggccacat ggccaaccag cacggcaagg tggcggccgc cgccatcgtg gccctgctgt
     4981 cgggtcgcca gcccaacccg ctgccgatct acaacaacac ctgctacagc ttcgtcagcg
     5041 acaccgacgt ggtgcatgtg gccagcgtgc acgcctacga cgccgacaag aagaccatgg
     5101 tgccggtggc cggctcgggc ggcgtgtcga gcgccgccag cgagctggaa gggcgctacg
     5161 ccatggcctg ggcgcgcaac atctgggccg atagcctggc atgacccccc tgagtcgctg
     5221 cgctccttcc cccctgcgag gggggacaac acctgtggcc ggcgcaggtc cggccacggt
     5281 gtttctggcg tggcctgctc cgcggccttc tgtccgatgg ctgcggcgcg tcggtgtcgc
     5341 ggtgctggcc gccctggccc tggcgcccgc actggcgcaa gacagcggtg aggcgcgcgc
     5401 gcgcaagatc atcggcggct cgtgctttct gtgccacggc gccgaaggcg aatcggccag
     5461 cgaggtgttc ccgcgcctgg ccggccagaa cgccgaatac atcgccaagc agctcgaaaa
     5521 cttcaagagc ggcaagcgca agagcacggc catggccagc atggtgaccg agctgacgct
     5581 cgacgacatg gtggcgctgg ggcgcttcta cgccgcccgc ccgccgcaca aggaggcggc
     5641 gcaggacgcc gcgctggccg gcgtggggcg ctacatctac cagtcgggca acaagtggag
     5701 cggcgtgcct tcgtgcgcca gctgtcacgg tgccgaaggg gctggcagca ccaccttgcc
     5761 gcgtctggcc gggcagcacg ccgcctacct ggaaacccag ctcaagcagt tcagcaagcg
     5821 cgagcgcaac aacgacaacg ccgtcatgca caccgtggtc gagaagatga ccggcctcga
     5881 aatggcggcg gtgtcggaat acctgagcgg caagtaaccc caggttaact cctgaaagta
     5941 tagctgctgg cgcaggcggg acgcgcgcca gcagcctttt tttctttgaa gccccaaaag
     6001 gcccgtaggg gcccggcgtt cagggtgcca ccggcagcac caccggtgcc tgctggctgg
     6061 ccggctggct ggtctgcacc ggctcgtcga gcagccggtg catcaggccg ccgccggccc
     6121 acatgccggc cagcgtcagc caggcggtga gggcgaagat ggcgccgccc gtcatgcccg
     6181 cgccgacggc gcagccaccg gccagcatgc tgccaaagcc catcagcacg gcgccggcga
     6241 tgtagcgcgc catgctgtag ccgtccttga agccttccac cttgaaatcg cgccccacca
     6301 gcgcgcccag cgccgagccc acgaacacgc ccggcaacat gccggcgtca aagccccagg
     6361 ctggcgccgg gctggccagc acgcgcatca gccattcggc cgacgggccg ctgaaggtca
     6421 ggccctgcat ctgcaccggg tcgaagctgg cgtgcgccac ggcataggtg aaccaccagg
     6481 ccagcgccac cgccagcccg ctgccgatgc cgccgatcca catccagggg cggttgccgc
     6541 tgcgcagcga aaagacgatg gccaccacca gccacaggcc gccgaacgcc aggccatcgg
     6601 cgtggcccgc gcccacgcgc gccagcagat cgcgcgcggc gccgccgtcc accgtccacc
     6661 agctcgagat ctcgctgcgc agcggcgcca gggcgccggc cagcgccgcc tgggcggtga
     6721 ccgcgaacac cagccccgac agcagcgcgc gcaggttgcc gttggccgac agcaccagca
     6781 gccggctggc gcagccgcgc gtcatgatca tgccggcgcc aaacaccagc ccgcccacca
     6841 gcgcgcccga caggctgccg cggttggcca gctggcgcgc ctcgcttacg ttcagcgcgc
     6901 cggccaggat cagcgcctgc accgccatca ccgccgccgc gaaggccagc agccagaccg
     6961 acagcttttc ggcgaactga ccgcgccaga actcgatcac ggcggcgcgc aggcaaaagc
     7021 gcgaacgctg ggcgaaaaat ccgaacagca tgccgatcag cgcgccgccg acggccagca
     7081 ccgtgccgct gccgtgctgc tcgaccaggc ttgtcaagtc gatggccatt cttgataatt
     7141 agttcagcaa tgacttatga atttagggca gatcatgcgg ccagtgattg accggcgtca
     7201 acttctccag cgctgcgcct gtctggcgct gggcggcatg ggggcagcct gggcgcgcta
     7261 cgccgaagcc gccgacaccc ccgccagtgc gccggcgatg caggcgcgcg aggccgcgcc
     7321 cggctgctgg tacgtcgaag gcctgtcggc cctgggctcg tcggccaacc agaacttcat
     7381 ctccaacgcc ggcttcgtgg tcacgtcgag cggtgtggtg gtgatcgacg ccctgggctc
     7441 acccgcgctg gccgagcgcc tgctggccga gattcgcaag gtgacggcgc agaaggtcac
     7501 gcacgtcatc gtcacccact accacgccga ccacatctac ggcctgcagg tgttcgaggc
     7561 gctgggcgcg cgcatcctgg cgcaccaggc ggcgcgcgaa tacctcaact ccgacaccgc
     7621 gcgcctgcgt ctgcaggcct cgcgcgagga actggcgccg tgggtcgatg cccgcacccg
     7681 cctggtgccc gccaccgaat ggatccacga gcggcgcgag ctgaccgtcg gcgacaccac
     7741 gctggtgctg caaccggtcg gcccggcgca cacgccggag gacctggcgg tgtacctgcc
     7801 gggggtcaag gcgctgtacg cgggcgatct ggtgtttcgc agccgcatcc ccttcgtcgg
     7861 ccaggccgac agccgcaact ggatcgtctc gctcgatcgc ctgctggcct tcgacacccg
     7921 ggtcatcatc cccggccacg gcccggtatc gaccgaggcg cgccaggaca tgcaactcac
     7981 gcgcgactac ctggtctatc tgcgcaagac catgggcgcc gccgcgcgcg acatgacccc
     8041 gttcgatgaa gcctaccggg ccaccgactg gagcatgtac gaacacctgc cgctgttcgg
     8101 cccggccaac cgcatgaacg cctacaacac ctacctgcag atggagcacg aaccgcaatg
     8161 aacacgccgt cccgcttctc gcccgcgcgc cgccatggcc tgcaggcgct ggccctgctg
     8221 ggcctgggtg ccggttgggc gccgccggcc caggcggccg gcaagacgct gccgctgtcg
     8281 gcttcgctgg ccgacgagct ggcgcgcgca ctgaaggcgc gccagccgct ggtggtgatg
     8341 gtcagcctgc accgctgccc ctggtgcgaa gaggtgcgca acaactacct ggcacccatg
     8401 cgcgcccagg acggcctgcc ggtggtgcag ctcgacatgc tcagcccgcg cgccacgcgc
     8461 gacctgcagg gcgcggccac cacgcacgac gcgctggtgc gcgcctggga cgtcaaggtc
     8521 gcgcccacgg tgctgtttct cggcccgcgc ggcaaggagg tcgccgaccg gctggtgggc
     8581 ggctcgcccg acttctattc cgcctacctc gaccgccgcc tggcgctggc ccgtgaagcc
     8641 gtggtagcct ag
//
LOCUS       DAOQTZ010000107         8639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_9064_length_8639_cov_13.068849, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000107 DAOQTZ010000000
VERSION     DAOQTZ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9064_length_8639_cov_13.068849"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKN51_11275"
     CDS             complement(<1..322)
                     /locus_tag="PKN51_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNT85724.1"
                     /translation="MSQDLASFTPATLADWRKAAAKSAPGGDLSALDWHTPDGITVKP
                     LYTAADVQGLPHADTLPGFEPFVRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRKAL
                     AAGGQ"
     gene            565..1203
                     /locus_tag="PKN51_11280"
     CDS             565..1203
                     /locus_tag="PKN51_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT85725.1"
                     /translation="MNSAARLAPRALYEEVAERLRQRIFSGELAPGDWIDELKIADEL
                     GISRTPLREALKVLAAEGLVTMKVRRGAYVTETSGKDLADVYHLLALLETDAAQAVAL
                     HADAAGRAELAALHAELEAAVGDAARFFALNEAFHLRLLELADNRWRLQMVQDLRKVM
                     KLHRRHSLQKAGRLQASLAEHRALMQAIAARDGAAAAACMQAHFQSGLEAAS"
     gene            complement(1235..1747)
                     /locus_tag="PKN51_11285"
     CDS             complement(1235..1747)
                     /locus_tag="PKN51_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNT85726.1"
                     /translation="MAYLHWVPELDTGIPEIDTQHKRILDYINKLHDLRDSPDRAALG
                     DVIGETVDYTLSHFAFEESVIESAGYMFAGPHKKVHELFTRKVAEMQSRFDAGEDVAA
                     ELHGMLSRWLFNHIRNEDHGYIDSVKAYLRMTSKQREADKAELKAELLQELAARQNGK
                     KGWFARLFGG"
     gene            1903..2790
                     /locus_tag="PKN51_11290"
     CDS             1903..2790
                     /locus_tag="PKN51_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT85727.1"
                     /translation="MKHYFMHGFYNKPSPQPDRRRALCALVGGGLTLTWAAQARADDG
                     GDWPRQAAVPGGVTRLELGPAPARPQAFADDAPLLVLGVPIAWSALVGIPLSAAPGTA
                     HIRVVTETGERRLPYSIAPRRYAEQHLKVAPRTVDLSPTDLARHERERAHQQGVIASF
                     SAPPEGSLRMRAPCTGPRSDSFGKRRFFNGQPRNPHSGLDIAAPTGTPVLAPLAGAVI
                     DTGDYFFNGQTVWLDHGGGLLSMLCHLSRIDCRPGEHLAAGQRLGAVGASGRVTGPHL
                     HWTVMLNRAAVNPALFLPA"
     gene            2916..3971
                     /locus_tag="PKN51_11295"
     CDS             2916..3971
                     /locus_tag="PKN51_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNT85728.1"
                     /translation="MLLLAAALAGCSAPPPAPPPAVAAPPAPAVVQAPALPQADSGAA
                     FTDRLGMRFVRVPAGEFMMGSAESPQALAKAFPHAERRRLTDLADEQPVHRVRLTRDF
                     WLGAHEVTVGQFRQFVQASGYVPESVRDGSGAYGFNPRYDPSRTQRGDLFEGRDPRYS
                     WQNPGFAQTDAHPVVNVTWNDAVAMARWLSEREGVTYRLPTEAEWEYAARAGSRTRYP
                     GGDDPQVLLRSANTFDRETALRWPRWRDQAGHGSDGHAFTAPVGSYAPNAFGLYDMIG
                     NVWEWCADWYGEDYYRRSPTDDPAGPASGKVRVRRGGSWHTWPLYARVAFRNWNTPET
                     RYVLLGFRLLREQGPAR"
     gene            3977..5158
                     /locus_tag="PKN51_11300"
     CDS             3977..5158
                     /locus_tag="PKN51_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNT85729.1"
                     /translation="MATGRARKVLIALALLALLGLAVLLGLPGFVDARMNAIALAPPY
                     PVSARAQQLHRRLFVADLHDDLLLWPRDPLQRHGHGQTDVPRLREGGVSLQVFTTVTK
                     TPRGMNFERNAGDSDNITLLAMAQRWPPRTWTSLLERALHQSRKLHQAARDSHGQLRL
                     VTSQAQLAAALADGLPAARGQLLAVLGTEGLHPLEGRLDHIDRLYEAGFRVMGLTHFF
                     DNEVGGSAHGLDKGGLTPFGHQVVRQLQQRRMIVDLAHASPAVIDDVLAIARRPVIVS
                     HTGVQAVCPGPRNLSDAQIRAIAARGGVMGMGFFQGAMCELSVERIVRSIRHVAQVAG
                     VQHVALGSDFDGATRTVFDGTGLALITEGLLQAGFSEAEIADIMGGNVLRLLQTELPA
                     N"
     gene            complement(5280..7868)
                     /locus_tag="PKN51_11305"
     CDS             complement(5280..7868)
                     /locus_tag="PKN51_11305"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HNT85730.1"
                     /translation="MQPVSTQSLLIKRARQEVVFLAGPLIDRCVEASISGLQEAERRG
                     RTTAIRMQLGDAWLALTRRRAELRDAFPPRVEQAMNQALEVARAAPSHSARAPLAHDE
                     LTLLEHAEVARFVEASRLQQSVMPLVEAPLARLDSLMSTALGLPVVRADLNPLRPDVL
                     CQALLALFDEQPVEAEWRVLWARHLAPSFAQELSQLYEAVTRLLVDQGVEEARYRLKL
                     NEDGASTGASVPVPASTKAQPSGGDGGAGGFAGGAAPGSADGGGARRRPIARMADLAQ
                     AEPAVSRTLMRDFLYRGQWTGEHDEPLPAAYYEAVGAQQQRLAAAPAAAYDALAAAQA
                     RALAQAQSVVDRPARRVEIGQSLAPEQWGELGSAQARTRTLMALKSRATWISQVLGLD
                     AVRALVGQVADDERVLAPVREAFVAMEPALLRLAMRDPRFLGDEQHPARRLVEAVAQR
                     SFRHNDEFADEFEQFMAPVRQAVRELDALPQARPQDFERHLQALEAGWQKQDEGEAEA
                     PEHGLRSIQFAQERQALADKVAWEFSLRSDLDGVPAVVADFLFQDWSLVIAHAQLTDT
                     RGQLDPGGYLAVVSDLLWSVKREATLKDPARAFEVLPEVVRTLRRGLDMLGKDPAEAQ
                     TFFDVLMRCHDPVLRLRRMRSARDAQASGQGPLTVISAAAPLDIVALPLERPTPRAAE
                     QPWLGRRELALAGFDHEAGDSGADSLHSSLNALSRPIESPSPDGAGDQADAAASASAP
                     LPADLPRPQPPDSLPLSEPAGAAADTGESAADASERARAQLAHLRTGDWVDLKVREQW
                     RRARLHWCSDNGALFMFISRGGQPHSMTRRSCEKLLRARKLRPVESGAVVERALSGLP
                     DAQPVT"
     gene            complement(8010..8429)
                     /locus_tag="PKN51_11310"
     CDS             complement(8010..8429)
                     /locus_tag="PKN51_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNT85731.1"
                     /translation="MSTVKPVSQAETTPRVKAVFDDIRATRGSEHINHFWRYLAFDPS
                     LLEATWAEVKQVMATPSALDPLTKELIYIAVSVANGCGYCVHSHTAAARAKGMSAAQH
                     ADLLAVVGLAARTNHLATALQVPVDPAFDRDAVPDRS"
BASE COUNT         1263 a         3149 c         2945 g         1282 t
ORIGIN      
        1 cctgcccgcc ggcggccagc gccttgcgat agaaggcgtt ggattcctcg gcggtggaaa
       61 agcccgcgta ctgccggatg gtccagggcc gcacggcgta catggtggcc tgcgggccgc
      121 gcacgaaggg ctcgaagccg ggcagcgtgt cggcatgcgg caggccctgc acgtcggccg
      181 cggtgtacag cggcttgacc gtgatgccgt cgggcgtgtg ccagtcgagg gcgctcaggt
      241 cgccgcccgg tgccgacttg gcggcggcct tgcgccagtc ggccagggtg gcgggtgtga
      301 aggaagcgag gtcttggctc atggcgggtt ccggttcggg gcggtctggt ggggcgtgcc
      361 gtggccgacg cgcccgcagg cgattgtgcg caagctgcgg cggctgtgtt gccggatggc
      421 catgaaggca ggtgcaacac cccgcaggcg cggcggctga ctttccataa ttattaatta
      481 aaaatgcagt gtctggctta caatggccgc aaattttttg cccgggcggg cggcccaacg
      541 tttcgccccg tcccggcccg ccccatgaac agcgccgccc gccttgcccc ccgcgccctc
      601 tacgaagagg ttgccgagcg cctgcgccag cgcatcttca gcggcgagct tgcgccgggc
      661 gactggatcg acgagctgaa aatcgccgac gagctgggca tcagccgcac gccgctgcgc
      721 gaggctctca aggtgctggc ggccgagggg ctggtgacga tgaaggtgcg gcgcggcgcc
      781 tacgtgaccg agacctccgg caaggacctg gccgacgtct accacctgct ggcgctgctg
      841 gaaaccgacg ccgctcaggc ggtggcgctg cacgccgacg ccgccggccg tgccgaactc
      901 gccgccctgc acgccgaact ggaagccgcg gtgggggatg cggcgcgctt ttttgcgctc
      961 aacgaagcct tccatctgcg cctgctggag ctggccgaca accgctggcg gctgcagatg
     1021 gtgcaggatc tgcgcaaggt gatgaagctg caccgccgcc attcactgca gaaggccggg
     1081 cgcctgcagg cctcgctggc cgaacaccgg gcgctgatgc aggccattgc ggcgcgcgat
     1141 ggcgccgccg ccgccgcgtg catgcaggca catttccaga gcggtctgga agcggccagc
     1201 tgaccgaccg cccggcggcg ccggcagggc cggctcagcc gccgaacagc cgggcgaacc
     1261 agcccttctt gccgttctgc cgagcggcca gttcctgcag cagctcggcc ttcaattcgg
     1321 ccttgtcggc ctcgcgctgc ttggaggtca tgcgcagata ggccttgacc gagtcgatgt
     1381 agccgtggtc ttcgttgcgg atgtggttga acagccaacg cgacagcatg ccgtgcagct
     1441 cggccgccac gtcctcgccg gcgtcgaagc ggctctgcat ctcggccacc ttgcgggtga
     1501 acagctcgtg caccttcttg tgcgggccgg caaacatgta gccggcgctt tcgatcacac
     1561 tttcttcgaa agcaaagtgc gacagcgtgt agtccaccgt ctcgccgatc acatcgccca
     1621 gcgcagcgcg atcggggctg tcgcgcaggt cgtgcagctt gttgatgtag tcgagaatcc
     1681 gcttgtgctg cgtgtcgatt tcggggatgc cggtgtccag ttcgggcacc cagtgaagat
     1741 aggccatggc aatcagtctc tgttggggaa tggtgcccag tattcaggga cccggcatga
     1801 atacttttga tttgagtcaa gcagtcaaga tctgttacca accgaggctc gatatgctcc
     1861 tgctttcata gcattcggcg cacactggtc cattccaaac ccatgaaaca ctacttcatg
     1921 catggttttt acaacaaacc gtctccgcaa cccgatcggc gccgtgcgct gtgtgctttg
     1981 gtaggcggcg gcctgaccct cacctgggcc gcgcaggcgc gagccgacga tggcggcgac
     2041 tggccacgcc aggccgccgt gccaggcggc gtgacacgcc tggagctggg cccggcaccc
     2101 gctcgaccgc aagccttcgc cgacgacgca ccgctgctgg tgctgggcgt ccccatcgcc
     2161 tggagcgccc tggtcggcat tccgctgtcg gccgcgcccg gcacggcgca catccgcgtc
     2221 gtcaccgaaa ccggggagcg tcgcctgccc tacagcatcg ccccgcggcg ctatgccgag
     2281 cagcacctga aggtggcacc gcgcacggtg gacctgtcac ccaccgatct ggcgcgccac
     2341 gaacgcgagc gcgcgcacca gcaaggcgtg atcgccagct tcagcgcgcc gcccgaaggc
     2401 agcctgcgca tgcgcgcgcc ctgcactggg ccgcgctccg attcgttcgg caagcggcgt
     2461 ttcttcaacg gccagccgcg caacccgcac agcggcctgg acatcgccgc gcccaccggc
     2521 acgccggtgc tggcgccgct ggccggcgcg gtgatcgata ccggcgacta cttcttcaac
     2581 ggccagaccg tctggctgga ccatggcggt ggcctgctgt cgatgctgtg tcacctcagc
     2641 cgcatcgact gccggccggg cgagcacctg gccgccggcc agcgcctggg cgccgtcggt
     2701 gccagcggtc gcgtgactgg gccgcacctg cactggacgg tgatgctcaa ccgcgccgcg
     2761 gtcaacccgg cgctgttcct gcccgcctga caggccaccc ccggcgccac atcgggattc
     2821 ctccgcattg acgcgccgac ggcgcactgc aagcatgggc cgccatgcgc cgacccctgc
     2881 ccaccccccg atcgtcatcc gccccaaccg ccctgatgct gctgcttgcc gcggcgctgg
     2941 ccggatgcag cgcaccgccg cccgcgccgc cgccggcggt cgccgcgccc cccgccccgg
     3001 ccgtggtgca ggcgccggcg ctgccgcagg ccgacagcgg cgccgccttc accgaccgcc
     3061 tgggcatgcg cttcgtgcgc gtgccggcgg gcgaattcat gatgggcagc gccgagtcgc
     3121 cgcaggcgct ggccaaggcc tttccgcacg ccgagcggcg ccgcctgacc gatctggcgg
     3181 acgagcaacc ggtgcatcgg gtgcgtctga cgcgcgactt ctggctcggc gcgcatgagg
     3241 tcacggtggg gcagttccgc cagttcgtgc aggcctcggg ctacgtgccc gagtcggtgc
     3301 gcgacggcag cggcgcctac ggcttcaacc cgcgctacga cccgtcgcgc acccagcgcg
     3361 gcgatctgtt cgaagggcgc gacccgcgct attcctggca gaacccgggc tttgcccaga
     3421 ccgacgcgca cccggtggtc aacgtcacgt ggaacgacgc cgtggccatg gcgcgctggc
     3481 tgagcgagcg cgagggcgtg acctaccgcc tgccgaccga ggccgaatgg gaatacgccg
     3541 cccgcgccgg cagccgcacg cgctacccgg gcggcgacga cccgcaggtg ctgctgcgca
     3601 gcgccaacac cttcgaccgc gaaaccgcgc tgcgctggcc gcgctggcgt gaccaggcgg
     3661 gccacggcag cgacggccac gccttcaccg ccccggtcgg cagctacgcg cccaacgcct
     3721 tcggtctgta cgacatgatc ggcaacgtgt gggagtggtg cgccgactgg tacggcgagg
     3781 actactaccg ccgctcgccc accgacgacc ccgccggccc ggccagcggc aaggtgcggg
     3841 tgcgccgcgg cggctcctgg cacacctggc cgctgtatgc gcgggtggca ttccgcaact
     3901 ggaacacgcc ggaaacgcgc tacgtgctgc tgggctttcg cctgctgcgc gagcaggggc
     3961 cggcgcgctg aacgccgtgg ccaccgggcg cgcgcgcaag gtcctgatcg cgctggcgct
     4021 gctggcgctg ctcgggctgg cggtgctgct tggcctgccg ggcttcgtgg acgcgcgcat
     4081 gaacgccatc gccctggcgc cgccttaccc cgtcagcgca cgcgcgcaac agctgcaccg
     4141 ccggctgttc gtggccgatc tgcacgacga cctgctgctg tggccgcgcg acccgctgca
     4201 gcgccacggc cacggccaga ccgacgtgcc gcgcctgcgg gaaggcgggg tcagcctgca
     4261 ggtgttcacc accgtgacca agacgccgcg cggcatgaac ttcgagcgca acgccggcga
     4321 cagcgacaac atcaccctgc tggcgatggc ccagcgctgg ccgccgcgca cctggactag
     4381 cctgctcgag cgcgcgctcc accagagccg caaactgcac caggcggcgc gcgacagcca
     4441 cggtcagttg cggctggtga cctcgcaggc gcagctggcg gcggcgctgg ccgacggcct
     4501 gccggccgcg cgcggtcagc tgctggccgt gctcggcacc gaagggctgc acccgctgga
     4561 aggccggctg gaccacatcg accgtctgta cgaggccggc tttcgcgtca tgggtctgac
     4621 gcatttcttc gacaacgagg tcggcggctc ggcccacggc ctggacaagg gcggtctgac
     4681 gccgttcggc caccaggtgg tgcggcaact gcagcagcgc cgcatgatcg tcgacctggc
     4741 gcacgcctcg cccgccgtga tcgacgacgt gctggccatc gcgcggcggc cggtgatcgt
     4801 ctcgcacacc ggcgtgcagg cggtctgccc gggcccacgc aacctgagcg acgcccagat
     4861 ccgcgccatt gccgcccgcg gcggcgtgat gggcatgggc ttcttccagg gcgccatgtg
     4921 cgagctcagc gtcgagcgca tcgtccgttc gatccgccac gtggcccagg tggccggggt
     4981 gcagcacgtc gccctgggct cggacttcga cggcgccacc cggacggtgt tcgacggcac
     5041 cggcctggcg ctgatcaccg agggcctgct gcaggccggc ttcagcgaag ccgaaatcgc
     5101 cgacatcatg ggcggcaacg tgctgcggct gctgcagacc gagctgccgg caaactgacg
     5161 cttgctactc taatgatagc tggtggtgct tgcccatggc gcgccacaag cctatttgat
     5221 ctgaaaccca gggtctgacg ccaagccgtc tcagccgcgg ccagcgtctg gccgcctgtt
     5281 caggtgaccg gctgggcgtc cggcagtccg ctcagggcgc gctcgaccac ggcgccggat
     5341 tccaccgggc gcagcttgcg cgcgcgcagc agtttttcgc agctgcgccg cgtcatgctg
     5401 tgcggctggc cgccgcgact gatgaacatg aacagggcgc cgttgtcgct gcaccagtgc
     5461 agtcgcgcgc gtcgccactg ctcgcgcacc ttgaggtcga cccagtcgcc ggtgcgcagg
     5521 tgcgccagct gggcacgggc gcgctcgctg gcgtcggcgg cgctctcgcc agtgtcggcc
     5581 gccgcgccgg cgggctcgga cagcggcagc gaatctgggg gctgaggccg cggcaggtcg
     5641 gcaggcagcg gcgccgatgc ggacgccgcg gcgtccgcct ggtcgcccgc gccgtcgggc
     5701 gacggcgact cgatcgggcg cgacaacgca ttgaggctgc tgtgcaggct gtccgcaccg
     5761 ctgtcgcccg cttcgtggtc gaagcccgcc agcgccagct cacgccggcc cagccagggc
     5821 tgctcggcag cgcgcggcgt gggccgttcc agcggcagcg ccacgatgtc caggggcgcg
     5881 gcggccgaga tcaccgtcag cgggccctgc cccgaggcct gggcatcgcg ggcgctgcgc
     5941 atgcggcgca gccgcagcac cgggtcgtgg cagcgcatca ggacgtcgaa gaaggtctgt
     6001 gcctctgccg gatccttgcc cagcatgtcc aggccacggc gcaaggtgcg caccacttcg
     6061 ggcagaacct cgaaggcgcg tgccgggtcc ttgagcgtgg cctcgcgctt cacgctccag
     6121 agcagatcgc tcaccaccgc caggtagccc ccggggtcga gctggccgcg cgtgtcggtc
     6181 agttgggcat gggcaatgac cagcgaccag tcctgaaaca gaaaatcggc caccaccgcc
     6241 ggcacgccgt cgaggtcgga gcgcaggctg aattcccagg ccaccttgtc ggccagcgcc
     6301 tggcgttcct gggcgaactg gatcgagcgc aggccatgct caggcgcctc ggcttcgccc
     6361 tcgtcctgct tctgccagcc tgcctccaac gcctgcaggt ggcgctcgaa gtcctgtggc
     6421 ctggcctgcg gcagggcgtc gagctcgcgc accgcctgac gtaccggcgc catgaactgc
     6481 tcgaactcgt cggcaaactc gtcgttgtgg cggaagctgc gctgggccac cgcctcgacc
     6541 agacgcctgg ccgggtgctg ctcatcgccc aagaaacgcg ggtcgcgcat ggccagccgc
     6601 agcagcgccg gctccatggc cacgaaggcc tcgcgcaccg gtgccagcac gcgttcgtcg
     6661 tcggccacct ggcccaccaa agcgcgcacc gcgtccagcc cgagcacctg gctgatccag
     6721 gtggcgcggg acttgagcgc catcagggta cgcgtgcgcg cctgggccga gcccagttca
     6781 ccccactgct cgggtgccag cgactgcccg atctcgaccc ggcgcgccgg ccggtcgacc
     6841 acgctctgag cctgggccaa cgcgcgcgcc tgggccgccg ccagcgcgtc gtaggccgct
     6901 gccggcgcgg cggccaggcg ctgctgctgc gcccccaccg cttcatagta ggcggccggc
     6961 aagggctcat cgtgctcgcc cgtccactgc ccccggtaca gaaagtcgcg catcagcgtg
     7021 cgcgataccg ccggctcggc ctgggccaga tcggccatgc gcgcaatcgg ccggcggcgc
     7081 gctcccccac catcggccga ccccggcgca gccccaccgg caaagccacc ggcgccgcca
     7141 tcaccgccgg agggttgcgc cttcgtggat gccggcaccg gcaccgacgc cccggtcgac
     7201 gccccgtctt cattcagctt caggcggtag cgcgcttctt ccacgccctg atccaccagc
     7261 aggcgggtca ccgcctcata cagctggctc agctcctggg caaaagacgg cgccaaatgg
     7321 cgtgcccaca gcacgcgcca ctcggcctcc accggctgct cgtcgaacag ggccagcagc
     7381 gcctgacaca gcacgtcggg acgcagcggg ttcagatcgg cccgcaccac cggcaggccc
     7441 agggccgtgc tcatcaggga atccagccgc gccagcggcg cctcgaccaa cggcatcacg
     7501 ctctgctgca gtcgcgatgc ctcgacaaaa cgcgccacct cggcgtgctc gagcagggtc
     7561 aactcgtcgt gcgcaagcgg cgcacgcgcc gagtgggagg gtgcggcgcg cgcaacctcc
     7621 agcgcctggt tcatggcctg ctccacgcga ggcggaaacg cgtctcgcag ctcggcgcgg
     7681 cggcgggtca gcgccagcca ggcgtcgccc agctgcatgc ggatggccgt ggtgcgaccg
     7741 cgacgctcgg cttcctgcag accgctgatg ctggcctcga cacagcggtc gatcagcggc
     7801 ccggccagga acacgacttc ctgccgcgcg cgcttgatca gaaggctctg ggtgctgacg
     7861 ggttgcatgg cgcaatagaa ctatcttacg caaattttcc cgtgggcgag ccatgggtgc
     7921 acacaatttg cgctggctca tataagcatt tggagttcag gcagcactcc acgccacacc
     7981 ggtccggtgg cgcccccgca ccgtgggtgt caggatcggt caggcaccgc gtcgcggtcg
     8041 aaggccggat ccaccggcac ctgcagcgcc gtcgccagat gattggtcct ggcggccagc
     8101 cccaccaccg ccagcaggtc ggcgtgctgg gccgccgaca tgcccttggc ccgtgccgcc
     8161 gcggtgtgcg aatgcacgca gtagccgcag ccattggcca ccgacacggc gatgtagatc
     8221 agctccttgg tcagcggatc cagcgccgaa ggcgtggcca tgacctgctt gacctcggcc
     8281 caggtcgctt cgagcagcga ggggtcaaag gccaggtagc gccagaaatg attgatgtgt
     8341 tccgaaccgc gcgtggcccg gatgtcatcg aacacggcct tgacccgcgg ggtggtctcg
     8401 gcctggctca cgggctttac ggtggacatg gtactcagcc ccccggcgtc gtgaccctgc
     8461 cggcgacgcg ctgccccccc aacccaccca gcgccgtcac acgccccggt ggccatacga
     8521 gcagcagcac accggccgct tggctggggc accaccccga actgattttt tcccgcatct
     8581 cggacccttc ctcgcgaacc ccatccgcgc gatgatagag agccctccag cgaccggca
//
LOCUS       DAOQTZ010000108         8495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_9251_length_8495_cov_14.211374, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000108 DAOQTZ010000000
VERSION     DAOQTZ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9251_length_8495_cov_14.211374"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..87
                     /locus_tag="PKN51_11315"
     tRNA            12..87
                     /locus_tag="PKN51_11315"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:His,seq:gtg)
     gene            complement(495..2417)
                     /locus_tag="PKN51_11320"
     CDS             complement(495..2417)
                     /locus_tag="PKN51_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNT85732.1"
                     /translation="MFDFVRRHTKLIMGVLFLLIIPSFVLFGIEGYTRFNDGATKVAT
                     VNGQPVTQMEWDSAHRLEIDRLRQGNPNLDLSLLDSPALKYATLERLVHERVLAAAAA
                     DEHLIATDARLAAELQRDPTIASLRRADGTLDMERYQQLLAAQGLTPAGFEANARHEL
                     SSRQVLGGVSETGFSSAAQAQVAMGAFQERREARVQRFAPAEFADKVQPTDADLQSYH
                     QTHASRYQAPETASVEYLVLDLESLKKSVSVSEQDLRTYYEQNAASFGTPETRRASHI
                     LLSAARDAPAAEREKAKAAATALLAQLRQAPDSFAELARKSSQDDASAASGGDLGRFE
                     RNKGMDPAIAQAAFGLAKVGDMSEVVESDFGFHIVRLTEIKAAEVPPFENMRAQLEDQ
                     LRTRLAQQQFSEMADEFRNLVYEQSDSLKPAADKLHLTIQTADKLSPTPAPGASGPLA
                     NPKFLGALFAADAVDKKRNTEAIDLGGNQIASGRIVSHTPAHARAFDEVRTEVRQAFV
                     RERGAELARQEGQARLKAWTAQPASATSLGAALVLSRDEPQGQPMALVEAVLRADPAK
                     LPGFVGVDLGAEGFAVARVDKVLAAPTQSAEQTAQSRARYDQLWGLAEARSYYELLKA
                     KYKAQILVPAPAPTAR"
     gene            complement(2502..2577)
                     /locus_tag="PKN51_11325"
     tRNA            complement(2502..2577)
                     /locus_tag="PKN51_11325"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2542..2544),aa:Val,seq:tac)
     gene            complement(2597..2869)
                     /locus_tag="PKN51_11330"
     CDS             complement(2597..2869)
                     /locus_tag="PKN51_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT85733.1"
                     /translation="MNKTELIEHIAKNADISKAAATRALEAAIGAVKTTLRKGGTVSL
                     VGFGTFAVGKRAARSGRNPRTGATIKIKAAKVPKFRPGKALKDALN"
     gene            complement(3181..4098)
                     /locus_tag="PKN51_11335"
     CDS             complement(3181..4098)
                     /locus_tag="PKN51_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT85734.1"
                     /translation="MNPSSPDASGATLIRLMPAVFVVIWATGFIVARYGMPHAPPFSF
                     LLLRYAFSVACFLPWIVLARVAWPRTGLEWLHLGVTGVLMHGGYLGGVWAAVKGGMGS
                     GLSALIVGIQPVLTAVWLAGMGGAGARVSARQWSGLLLGFAGLVLVVWRKLTHGAPGD
                     HVTATNLAWAVLALVSITAGTLYQKRFVRPCDVRTANTVQLLAAALVTAPLAWLEPES
                     VRWNGQVVGALAWSVLGLTLGGSSLLYLLIQRGAASSVASLMYLVPPTTAVMAWALFG
                     EPIGVTTLAGIALTAFGVSLVVRPAAGRP"
     gene            complement(4106..4672)
                     /gene="pgsA"
                     /locus_tag="PKN51_11340"
     CDS             complement(4106..4672)
                     /gene="pgsA"
                     /locus_tag="PKN51_11340"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNT85735.1"
                     /translation="MFFTLPTLLTWTRIVAIPLIVGVFYLPLSAPTANLAATVMFMVF
                     AWTDWLDGFLARKLNQTSAFGAFLDPVADKFLVCASLLVLVHLQRTDVFVALIIIGRE
                     IAISALREWMAKIGASKSVAVHMVGKLKTAVQMVAIPFLLYDGVLLERIDARWWGNLL
                     IWVAAVLTVWSMVYYLRKALPEIRARVQ"
     gene            complement(4800..6776)
                     /gene="uvrC"
                     /locus_tag="PKN51_11345"
     CDS             complement(4800..6776)
                     /gene="uvrC"
                     /locus_tag="PKN51_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNT85736.1"
                     /translation="MTDAPAPDDAPPPPPAAPRHRPELLAQVAALPGLPGVYRYYDAA
                     DNVLYVGKARNLKRRVSSYFQKNHGGTRIGHMVGKIARLETTVVRSEAEALILENNLI
                     KQLAPRYNILFRDDKSYPYLKITGSEGVPDRGEGAPRPEAYARMTYYRGATDRRSRYF
                     GPYPSAWAVKETIELLQKVFRLRTCEDTVFAHRTRPCLLYQIKRCTAPCVRLIGQADY
                     AADVAQAERFLRGQTQQLLTEMERRMMAHADALEFEQAAAIRNQIQSLSRVLHQQAVE
                     VGGEADVDIIAVKVQGGRACVNLAMVRGGRHLGDRAHFPSQVEDAHALAGEREDAELT
                     SVEAQVLDAFIAQHYLALPTPPQLITSEPVTRTLLQALSDKEERRISATHAPRGQRRV
                     WLEMAQQNAALQLARLLAEEGSQQARTRALAEALELPTEALDQLRIECFDISHTAGEA
                     TQASCVVFEGHRMASSEYRRYKIDGITPGDDYAAMRQVLQRRYGKIAEALREQGNAAS
                     VRMPDLVLVDGGKGQVSVAREVFEALALDLSLIVGVEKGEGRKVGLEELVFADGRPKV
                     YLGHDSAALMLVAQIRDEAHRFAITGMRAQRAKVRVGGSQLEDIPGVGPRKRARLLQR
                     FGGVRGVEAASIDDLATVEGISRALAEDIYRALH"
     gene            6802..7977
                     /gene="earP"
                     /locus_tag="PKN51_11350"
     CDS             6802..7977
                     /gene="earP"
                     /locus_tag="PKN51_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNT85737.1"
                     /translation="MAPPRRALPHNASVPEPHPPLRWDIFCQVIDNHGDLGVCWRLAT
                     ALAAREQQVRLWVDDARALDWMAPAATRPTGVQVLPWTRPLDPMRLAGLPPADVWVEA
                     FGCQIATEFIAAGAERISAANHKGLKPPVWINLEYLSAEGYVERMHGLPSPVLQGPAR
                     GWTKWFFYPGFTPATGGLLREDDLPARQAAFDRRAWLAGQGIDWQGERLISLFCYEPP
                     ALATWIAQLRGSPEPTRLLVTAGRARQAVEQVLEQENRLQRPSSQRQQLSISYLPRFT
                     QIAYDELLWACDLNFVRGEDSLVRALWAGQPLVWHIYPQPEDNAHHAKLRAFLDWLEA
                     PASLRQFHLAWNGIDGGALPAPDLPAWRRGVQAARQRLHAQTDLATQLLRFVARQTL"
     gene            8112..>8495
                     /locus_tag="PKN51_11355"
     CDS             8112..>8495
                     /locus_tag="PKN51_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNT85738.1"
                     /translation="MKLAQEIRAGNVIMHGKDPMIVLKTEYARGGRGAATVRMKLKAL
                     LSNMGTEVVFKADDKMDQVILDHKECTYSYFADPMYVFMDGEYNQYEVEAGNMGDALS
                     YLEDGMAVEVVFYDGKAISVELPTSV"
BASE COUNT         1354 a         2928 c         2836 g         1377 t
ORIGIN      
        1 ggtactcagt ggtggctgta gctcagttgg tagagtccag gattgtgatt cctgtcgtcg
       61 tgggttcgag tcccatcagc caccccaccg aatcggcgcc catggccctg cacaaagggt
      121 catgggcttt ttctggcctc tgaccctagg aggctgtcgg acttggaaat cgccggctgc
      181 aaatcgaccg cagcggccca tttttggctt cgccgctctt cgagagcacc ggctttttgt
      241 ccaattgctc ggctattggg ctgcaaatcc ggcaaaaact ggtctcgctg gggccgatct
      301 tcgctttcgg cgctccaagc ccgacagcct cctaggctga ccgatcgcca ctgagcgcgc
      361 gacacctgca gcgccgcggg caacgatgct cccgccttcg atccggctga gcctgacgca
      421 caaggcccgc ccgtgctctg tcgcaaaaaa agcgccgatg gcgcttttca tgcatgggtc
      481 gacggggccg cgactcaacg cgccgtcggg gcaggggccg ggaccaggat ctgggccttg
      541 tacttcgcct tgagcaactc gtagtagctg cgggcctcgg ccagacccca cagctggtcg
      601 tagcgcgccc ggctctgggc cgtctgctcg gcgctctggg tcggcgcagc cagcaccttg
      661 tcgacacgtg cgacggcaaa cccttccgcc ccgaggtcga cgccgacgaa accgggcagc
      721 ttggcggggt cggcccgcag cactgcctcc accagcgcca tcggctggcc ctggggttcg
      781 tcgcgcgaca gcaccaacgc cgcacccagt gaggtggccg atgccggctg cgccgtccag
      841 gccttcagac gggcctgacc ttcctggcgc gccagttcgg cgccgcgttc acgcacgaag
      901 gcctgacgca cctcggtcct gacttcgtcg aaggcgcgcg catgcgccgg ggtgtgcgac
      961 acgatgcggc cggaggcgat ctggttgccg ccgaggtcga tggcctcggt gttgcgcttc
     1021 ttgtcgacgg cgtcggcggc aaacagggcg cctaggaact tcggattggc cagcggcccg
     1081 ctggcgcccg gcgccggcgt gggcgacagc ttgtcggccg tctggatggt caggtgcagc
     1141 ttgtcggccg cgggcttgag gctgtccgac tgttcgtaca ccaggttgcg gaactcgtcg
     1201 gccatttcac tgaactgctg ctgcgccagg cgcgtgcgca gctggtcttc cagctgggcc
     1261 cgcatgttct cgaacggcgg cacctcggcc gccttgatct cggtcagccg tacgatgtga
     1321 aagccgaaat cgctttccac gacttcgctc atgtcgccca ccttggccag cccgaacgcg
     1381 gcctgggcga tggccggatc catgcccttg ttgcgctcga agcgccccag atcgccgccg
     1441 ctggcggcgc tggcgtcgtc ctgagaggac ttgcgcgcca gctcggcaaa gctgtcgggc
     1501 gcctggcgca gctgcgccag cagggcggtg gccgccgcct tggccttttc gcgctcggcg
     1561 gccggtgcgt cgcgcgccgc gctcagcaga atgtggctgg cgcgccgcgt ctccggcgta
     1621 ccgaagctgg cggcgttctg ctcgtagtag gtgcgcaggt cctgctcgct cacgctgacg
     1681 cttttcttca ggctctccag gtccagcacc aggtactcca cgctggcggt ttccggcgcc
     1741 tggtagcgcg acgcatgggt ctggtggtag ctctgcagat cggcgtcggt cggctggacc
     1801 ttgtcggcaa actcggccgg ggcaaagcgc tgcacgcggg cttcgcggcg ctcctgaaac
     1861 gcccccatgg cgacctgggc ctgggccgcg ctggaaaacc cggtctcgga caccccgccc
     1921 agcacctggc gcgacgacag ctcgtgtcgc gcattggctt cgaagccggc cggcgtcagc
     1981 ccctgggcgg ccagcagctg ctggtagcgc tccatgtcca gggtgccatc ggcgcggcgc
     2041 aggctggcga tggtggggtc gcgctgcagt tcggccgcca ggcgggcgtc ggtggcgatc
     2101 agatgctcgt cggcggccgc ggcggccagc acacgttcgt gcaccagccg ctccagcgtc
     2161 gcgtacttga gcgctggcga atcgagcagg ctcaggtcca ggttcgggtt gccctgccgc
     2221 aggcggtcga tctccagccg atgcgcgctg tcccattcca tctgggtgac cggctgaccg
     2281 ttgaccgtgg ccaccttcgt ggcaccatcg ttgaaacggg tgtagccctc gatgccgaac
     2341 agcacgaagg acggaatgat cagcaggaac agcacgccca tgatgagctt ggtgtgccgg
     2401 cgaacgaaat cgaacatgcg cgagactctc ggtgtacgcg gtggcgtaaa aacgaaaaag
     2461 gcgaaccggg gttcgccttt gctgcaatcg gtggaggatg ctggtgggtg ctgacggggt
     2521 cgaaccgccg acctacgcct tgtaagggcg ccgctctacc aactgagcta agcaccccag
     2581 ccggaccgac atggcatcag ttcagcgcgt ccttcaaggc cttgccggga cggaacttgg
     2641 gcaccttggc tgccttgatt ttaatcgttg cgcccgtgcg ggggttgcgg cccgagcgcg
     2701 ccgcacgctt gcccacggca aaggtgccaa aacccaccag cgacaccgtt ccacccttgc
     2761 gcagcgtggt cttgacggcg ccgatggccg cctccagggc acgcgtggcg gcggccttgg
     2821 aaatgtccgc attcttggcg atgtgctcga tcagctcggt cttgttcacg aatgtgtccc
     2881 tctcgaaaaa attcaaggac caggccagaa cggcatgcgt gtctggcaag ccggtccaac
     2941 ccggttttcc tttgcttggc ccatcgccgc agcagggcgt ttcaatcaca cggtcaccca
     3001 cgtgacactg gttctgcgct gacgcaatcc ccagacggcg agggacgcat tctagactca
     3061 attgcccagg ccaccggggg gaaatccggt ctgccgctgc gccgctacaa cggctgaacg
     3121 tgatttttga ctggtgcctg tgccgcccag ggcacagcgg ccgagcctgg gccgaccagc
     3181 ttagggcctg ccggcggcgg gccgcaccac cagactgacg ccaaaggccg tcagtgcgat
     3241 gccggccagc gtggtgacgc cgatcggctc accgaacagc gcccaggcca tcaccgccgt
     3301 ggtaggcggc accagataca tcaggctggc cacgctggac gccgcgccgc gctgaatcag
     3361 caggtacagc agcgagctgc cgcccagtgt caggcccagc accgaccagg ccagcgcccc
     3421 caccacctgg ccgttccacc gcacggattc aggctccagc caggccagcg gcgccgtcac
     3481 cagggctgcg gccagcagct gtacggtgtt ggcggtgcgc acgtcgcagg gacgcacgaa
     3541 gcgcttctga tagagcgtgc cggcggtgat gctgaccagg gccagcaccg cccaggccag
     3601 gttggtggcg gtgacgtggt cgcccggcgc gccatgcgtc agcttgcgcc acaccaccag
     3661 caccagcccg gcaaagccca gcagcagccc gctccactgg cgcgcgctga cgcgggcgcc
     3721 ggccccaccc atgcccgcca gccagacggc ggtgagcacc ggctggatgc cgacgatcag
     3781 cgccgacagg cccgaaccca tgccgccctt gaccgccgcc cacacgccgc ccaggtagcc
     3841 gccgtgcatc agcacgccgg tgacgcccag gtgcagccat tcgaggccgg tgcgcggcca
     3901 ggccacacgg gccaggacga tccacggcag gaagcaggcc accgaaaacg cgtagcgcaa
     3961 cagcagaaaa ctgaacggcg gcgcatgcgg catgccgtag cgcgcgacga tgaagccggt
     4021 ggcccagatg acgacgaaga cggccggcat caggcggatc aacgtggcgc cgctggcgtc
     4081 cggggaagaa gggttcatgc gggcgtcact gcacgcgggc gcggatttcg ggcagggcct
     4141 tgcgcaggta gtacaccatc gaccacacgg tgagcaccgc cgccacccag atcagcaggt
     4201 tgccccacca gcgcgcatcg atgcgctcca gcagcacgcc gtcgtacagc aggaacggga
     4261 tggccaccat ctgcaccgcg gtcttgagct tgcccaccat gtgcacggcc acgctcttgc
     4321 tggcgccgat cttggccatc cattcgcgca gggcagaaat ggcaatctcg cgtccgatga
     4381 tgatcagcgc cacgaacacg tcggtgcgct gcaggtgcac cagcaccagc aggctggcgc
     4441 agaccaggaa tttgtcggcc accgggtcga gaaaggcgcc gaaggccgag gtctggttca
     4501 gcttgcgggc cagaaagccg tccagccagt cggtccaggc gaacaccatg aacatcacgg
     4561 tggccgccag gttggcggtc ggcgcggaca gaggcaggta gaacacaccc acgatgagcg
     4621 gaatcgcgac gatgcgcgtc caggtcagca gcgtgggcag ggtgaagaac atggcggcga
     4681 ttgtgtcacg cggcttgcgg gcacgcagcg tgctccgcca aggccctgca tgctatgtaa
     4741 acgatagctg cctgcgctta ctacccctgc gccagatgcc gatttgatgc gcaatccggt
     4801 caatgcagcg cacggtagat gtcctcggcc agcgcacgcg agatgccttc gaccgtggcc
     4861 agatcgtcga tgctggcggc ctccaccccg cgcacgccac caaagcgctg cagcaggcgc
     4921 gcacgcttgc ggggcccgac gccggggatg tcttccagct ggctgccgcc cacgcgcacc
     4981 ttggcgcgct gcgcacgcat gccggtgatg gcgaagcggt gcgcctcgtc gcggatctgc
     5041 gccaccagca tcagcgcggc cgagtcgtgg cccagataga ccttgggccg gccgtcggca
     5101 aacaccagct cttccagccc gaccttgcgg ccttcgcctt tttcgacgcc gacgatcagc
     5161 gacagatcga gcgccagcgc ctcgaacact tcgcgcgcca cgctgacctg gcccttgccg
     5221 ccatcgacca gcaccagatc gggcatgcgc acggaggcgg cgttgccctg ttcgcgcagc
     5281 gcctcggcga tcttgccgta gcgccgctgc agcacctggc gcatggcggc gtagtcgtcg
     5341 ccgggggtga tgccgtcgat cttgtagcgc cggtactcgc tgctggccat gcgatggcct
     5401 tcgaacacca cgcaactggc ctgcgtggcc tcgcccgcgg tgtggctgat gtcgaagcat
     5461 tcgatgcgca gttgatccag cgcctcggtc ggcagctcca gcgcctcggc cagcgcccgc
     5521 gtgcgcgcct gctgcgagcc ttcttcggcc agcaggcgcg ccagctgcag ggcagcgttt
     5581 tgctgcgcca tctccagcca tacgcggcgc tggccgcgcg gcgcatgggt ggcgctgatc
     5641 ctgcgctctt ccttgtccga caaggcctgc agcagcgtgc gcgtcaccgg ttcgctggtg
     5701 atcagctggg gcggcgtcgg cagcgccagg tagtgctggg cgatgaaggc gtccagcacc
     5761 tgcgcctcga cgctggtgag ctcggcgtcc tcgcgctcgc cggccagtgc gtgcgcatct
     5821 tcgacctgac tgggaaagtg ggcgcgatcg cccaggtggc gcccaccgcg caccatggcc
     5881 aggttgacgc aggcgcgtcc gccctgcacc ttgacggcga tgatgtcgac gtcggcttca
     5941 ccacccacct ccaccgcctg ctgatgcagc acgcgcgaca gcgactggat ctggttgcgg
     6001 atcgccgccg cctgctcgaa ctccagcgcg tcggcgtgcg ccatcatgcg ccgttccatc
     6061 tcggtcagca gctgctgcgt ctggccgcgc agaaagcgct cggcctgggc gacgtcggcg
     6121 gcgtagtcgg cctggccgat cagccgcacg cagggcgcgg tgcagcgctt gatctggtag
     6181 agcaggcagg gccgcgtgcg atgggcgaac accgtgtcct cgcaggtgcg caggcgaaac
     6241 accttttgca aaagctcgat ggtttccttg accgcccagg cactgggata gggaccgaag
     6301 tagcggctgc ggcggtcggt ggcgccgcgg tagtaggtca tgcgcgcgta ggcctcgggt
     6361 cggggcgcgc cctcgccgcg gtccggcacg ccttcgctgc cggtgatctt caggtagggg
     6421 tagctcttgt cgtcccgaaa caggatgttg tatctgggcg ccagctgctt gatcaggttg
     6481 ttttccagaa tcagcgcctc ggcctcggag cgcaccacgg tggtttccag ccgcgcgatc
     6541 ttgccgacca tgtggccgat gcgcgtgccg ccgtggtttt tctgaaagta gctgctgacg
     6601 cggcgcttga ggttgcgcgc cttgcccacg tacagcacgt tgtcggccgc atcgtagtag
     6661 cggtacacgc cgggcagacc gggcagcgcc gccacctgcg ccagcagctc ggggcggtgg
     6721 cgcggcgcgg cgggtggcgg gggcggcgcg tcgtcgggcg ccggggcatc ggtcatggtg
     6781 cgcagtgtac gaagccacgc catggcgccg ccacggcggg ccttgcccca caatgccagc
     6841 gtgcccgaac cccacccccc gctgcgctgg gacatcttct gtcaggtcat cgacaaccac
     6901 ggcgacctgg gcgtctgctg gcgcctggcg acggcgctgg cggcgcgtga gcagcaggtg
     6961 cgcctgtggg tggacgacgc gcgcgcgctg gactggatgg cgcccgcggc cacacgcccg
     7021 accggcgtgc aggtgctgcc gtggacgcga ccgctggacc ccatgcggct ggccgggctg
     7081 ccgccggccg atgtctgggt ggaagccttc ggctgccaga ttgctactga attcatagct
     7141 gccggcgctg aaaggataag cgctgcgaac cacaaaggct tgaaaccacc ggtatggatc
     7201 aacctcgagt acctgagcgc cgaggggtac gtcgagcgca tgcacggcct gccctcgccg
     7261 gtgctgcagg ggcccgcccg cggctggacc aagtggttct tctaccccgg cttcaccccg
     7321 gccaccggtg ggctgctgcg cgaagacgac ctgccggcgc gccaggccgc cttcgatcgc
     7381 cgcgcctggc tggccgggca aggcatcgac tggcaaggcg agcggctgat ctcgctgttc
     7441 tgctacgagc cccccgcgct ggcgacctgg atcgcgcagc tgcgcggctc gcccgaaccg
     7501 acgcgcctgc tggtcaccgc cggccgcgcc aggcaggcgg tggagcaagt gcttgagcag
     7561 gaaaaccggc tgcagcgccc ttccagtcag cgccagcagc tatcgatttc atacctgccg
     7621 cggttcacgc aaatcgccta cgacgaactg ctgtgggcct gcgacctgaa cttcgtgcgc
     7681 ggcgaagact cgctggtgcg tgcgctgtgg gccggccagc ccctggtctg gcacatctac
     7741 ccgcaacccg aggacaacgc ccaccacgcc aagctgcgcg cctttctgga ctggctggaa
     7801 gcgccggcca gcctgcgcca gttccacctc gcctggaacg gcatcgacgg tggcgccctg
     7861 cccgcccccg acctgcccgc ctggcgccgc ggcgtacagg cggcccgcca gcgcctgcac
     7921 gcgcagaccg atctggccac ccagctgctg cggttcgtgg cgcggcaaac gttataattc
     7981 aaggctttgc aaaatcagct gccaagtcgt gagcgcggct gtcttttgcc acgctgccgc
     8041 cgcgcgcacg ccctttctca cagccagtgc acgagcggcc cacgctcacc tcacacactg
     8101 gaatcacagc catgaaactc gcccaggaaa tccgtgccgg caacgtcatc atgcacggca
     8161 aggacccgat gatcgtcctc aagaccgaat acgcccgcgg cggccgcggc gccgccaccg
     8221 tgcgcatgaa gctcaaggcc ctgctgtcca acatgggcac cgaagtggtg ttcaaggccg
     8281 acgacaagat ggaccaggtc atcctggacc acaaggaatg cacctattcc tactttgccg
     8341 accccatgta cgtgttcatg gacggcgagt acaaccagta cgaggtcgag gccggcaaca
     8401 tgggcgacgc cctgagctac ctggaagacg gcatggcggt cgaagtggtg ttctacgacg
     8461 gcaaggccat ctcggtcgag ctgcccacca gcgtc
//
LOCUS       DAOQTZ010000109         8367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_9437_length_8367_cov_13.099976, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000109 DAOQTZ010000000
VERSION     DAOQTZ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9437_length_8367_cov_13.099976"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PKN51_11360"
     CDS             complement(<1..1121)
                     /locus_tag="PKN51_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HNT85739.1"
                     /translation="MNTSVATANPTSVTLDGGFFARRTWGDWLFALLVLAGALFAFQR
                     YHASMDVYEKWILAGTAPVLIGLGWFWRPLRTLMLVVAGLSLLAVLSYAGDLRRADSV
                     FWLKYFLSSQSAILWMSMIFFMSTVFYWLGMFSRGQSEALEGIGSKLLWVAVTLALVG
                     TLVRWYEGHQMGPDIGHIPVSNLYEVFVMFCWMTALFYLYYESQYRTRAMGAFVMLVV
                     SAAVGFLLWYTLVREAHEIQPLIPALQSWWMKLHVPANFIGYGTFALAAMLGFAYLIK
                     QQAEETRWYKLAPIWVFGIALCFVPIAFRQRGVGEAGGSYWLGYALVAALIVGTILLG
                     RRRIAARLPSFEVLDDVMYKSIAVGFAFFTIATVLGALWA"
     gene            complement(1118..3244)
                     /locus_tag="PKN51_11365"
     CDS             complement(1118..3244)
                     /locus_tag="PKN51_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNT85740.1"
                     /translation="MTASHSTHGYQPRHGSAAWRASVELLSSMRFAIALLTVICIASV
                     IGTVIKQHEPAPNYVNQFGPFWAELFLKLQLNAVYSAWWFLLILAFLVVSTSLCVLRN
                     TPKFLADIRSYKENIRHQSLQAFHHKAEGELDESPEVAARRIGQQLAGGGWKVRLQAR
                     DTPEGQGWMVAAKAGAANKIGYIAAHSAIVLICLGGLFDGDLFVRAMTWLGGKSVYSG
                     GGLVSQVRPEHRLPETNPAFRGNLVVSEGTQSSTAILSQSDGVLLQELPFAVELKKFI
                     VEYYATGMPKLFASEIVIHDKATGQTQPARVEVNHPASYKGVQIYQSSFDDGGSRVKL
                     RAVPMNGAAKAFEVEGTIGGNAQITNGSDKLTLEYTALRVINVENLSEGARGSGADVR
                     KVDLGQALTQRLGAADKTVTQRELRNIGPSITYKLRDAAGQANEFHNYMLPVDMGDGG
                     AVFLLGVRASQAEGFRYLRVPADEDGAIDGFVRLRGALQDAAQREQAVRRYVRKAVDP
                     QRPELVEQLAQSASRALGLFAGAEAVNGRTTGGLQAVSDFIETAVPESERERAAEVLL
                     RILNGSLLELNDLARQAQGKPPLTESEASRRFMTQAVLALSDAPLYPAPVAFELTGFE
                     QVQASVFQVARAPGKNVVYLGCLLLILGVFAMLYVRERRLWVWLAPRGAGAQASMALS
                     SNRKLLDVDREFEQWQRRLLRRESEL"
     gene            complement(3430..4047)
                     /locus_tag="PKN51_11370"
     CDS             complement(3430..4047)
                     /locus_tag="PKN51_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNT85741.1"
                     /translation="MKSLAALLTASLLAATASAADAPAAPKVDLAKGAASYATVCVAC
                     HAADGNSTVPLQPSLAQQHPEYTLKQLLEFKSGARKDPVMQGFAAMLSEDDARNISWW
                     LAAQKAKPGAASDKDLVLTGERIFRGGIPDRHIPACAGCHSPNGAGIPSQYPRLSGQH
                     TDYTVKQMVAFRDGTRANNAVMQGVAAKMNDREIKAVSDYIAGLR"
     gene            4170..4880
                     /gene="yihA"
                     /locus_tag="PKN51_11375"
     CDS             4170..4880
                     /gene="yihA"
                     /locus_tag="PKN51_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNT85742.1"
                     /translation="MTARPAPAPAAAAARPDAATPAAAAREALGWLHTARFLTTAAQL
                     HQLPALAVPEIAFVGRSNAGKSTAINTLTQQTQLAFASRKPGRTQHINLFALGRQGGT
                     DAVLADLPGYGYAAVPLADKQRWQRVMANYLVTRENLAAVVMLCDPRHGLQELDEILL
                     EVIRPRVEQGLPFLILLTKADKLNRSEQARALQIARLQAGGGSVMLFSAPKRQGVEDA
                     ATWLRAVVDAAANPSDAA"
     gene            5027..5941
                     /locus_tag="PKN51_11380"
     CDS             5027..5941
                     /locus_tag="PKN51_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNT85743.1"
                     /translation="MIETWLAELPHGITLSCRGAGARGRPLMLLLHGFPEAAFIWDAL
                     MAHFAQPEHGGFRCVAPNLRGFERSSAPAEVAAYKPALMVQDVLALAAAEGVDGRIHA
                     LVAHDWGGAFAWAAAHAHPAQVDHLVIINSPHPATFARELQRSPEQQQASAYMNFLAR
                     PDAEALLAEDDYRRLWPFFTSMGAGPERFGWLTEAVRQQYRDVWRHGLTGACNLYRVT
                     PLKPPAPGQRGVTDIPQPPPERVRVEVPTLVLWALDDTALRPGLLDGLDAHVPRLTVQ
                     RMPGATHWVVHEQPQQVAAHIGAFVTTK"
     gene            complement(6108..6986)
                     /locus_tag="PKN51_11385"
     CDS             complement(6108..6986)
                     /locus_tag="PKN51_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HNT85744.1"
                     /translation="MTTHPGVWLMRALAPLPLPWLRALGDVLGRLLFALARRRRHIAL
                     VNLRLAFPAWSEAERMDVARRSFIAFAQSFIDRAWVWHAPDAVVRQRIRLTGAVDELR
                     RPEAAVLFAPHFYGMDAGGAAIMQQAVAKGGSIYSPQSGAATDAWVRAGRERFGDVVL
                     INRRDGVKPVVKSLREGRYLYLLPDMDLGPEESVFVPFFGVPAATVPSLPRLARLGRA
                     RVVPTVTRMTPGGYEVQVHPAWSGYPGDDPVADTALMNRRLEDWIRTMPEQYYWVHKR
                     FKTRPAGEPSVYGRTP"
     gene            complement(6983..7816)
                     /locus_tag="PKN51_11390"
     CDS             complement(6983..7816)
                     /locus_tag="PKN51_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT85745.1"
                     /translation="MLALYRLAARWPLSLLHALGAALGWLSWLASPTYRRRLRDNAAL
                     AGYGFAQVRPAVAHAGRMALETPRLWFGPPVPMRWDGTELVDAAYAAGRGVVFLTPHL
                     GCFEITAQGLAERYHARYGPITVLYRPARQAALAQVVETARRREGLDTAPTTLAGVRQ
                     MIKALRAGRAVGLLPDQVPPEGQGLWSPFFGQPAYTMTLAARLIQQTGAVPLLVWGER
                     RPRGQGYDLHFRALAEPLADELAPAVAQINREMERLIRECPTQYLWGYGRYKQPREQP
                     A"
     gene            7961..>8367
                     /locus_tag="PKN51_11395"
     CDS             7961..>8367
                     /locus_tag="PKN51_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNT85746.1"
                     /translation="MANSFLFTSESVSEGHPDKVADQISDAILDAILAQDERSRVAAE
                     TLTNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSH
                     DIAQGVDHASDDHLNTGAGDQGLMFGYACDETDT"
BASE COUNT         1309 a         3014 c         2866 g         1178 t
ORIGIN      
        1 gcccacagcg cgcccagcac ggtggcgatg gtgaagaagg caaagccgac cgcgatggac
       61 ttgtacatca cgtcgtccag cacctcgaag ctgggcaggc gtgcggcgat gcgccggcgc
      121 cccagcagga tggtgccgac gatcagcgcc gccaccagcg cgtagccgag ccagtagctg
      181 ccgccggcct cgcccacgcc gcgctgacgg aaggcgatcg gcacgaagca cagcgcgatg
      241 ccgaacaccc agatcggcgc cagcttgtac cagcgcgtct cctcggcctg ctgcttgatc
      301 aggtaggcaa agcccagcat ggcggccagc gcgaaggtgc cgtagccgat gaagttggcc
      361 ggcacgtgca gcttcatcca ccagctctgc agcgccggga tcagcggctg gatctcgtgc
      421 gcctcgcgca ccagcgtgta ccacagcaga aagccgaccg ccgcgctgac caccagcatg
      481 acgaaggcgc ccatggcgcg ggtgcggtac tgcgactcgt agtagaggta gaacagcgcc
      541 gtcatccagc agaacatgac gaacacttcg tacaggttgc tgaccgggat gtggccgatg
      601 tccggcccca tttggtggcc ttcgtaccag cgcaccaggg tgcccaccag cgccagcgtc
      661 accgccaccc acagcagctt gctgccgatg ccctccagtg cctcgctctg gccacgcgag
      721 aacatgccga gccagtagaa caccgtgctc atgaagaaga tcatgctcat ccacaggatg
      781 gccgactggc tggacaggaa gtacttgagc cagaacaccg aatcggcgcg ccgcagatcc
      841 cccgcatacg aaagcacggc cagcagcgac agccccgcca ccaccagcat cagcgtgcgc
      901 agcgggcgcc agaaccagcc caggccgatc agcaccgggg cggtgccggc cagaatccac
      961 ttttcgtaca cgtccatcga cgcgtggtag cgctggaacg cgaacagcgc gcccgccagc
     1021 accagcagcg cgaacagcca gtcgccccag gtgcggcgcg cgaaaaagcc cccgtccagg
     1081 gtgacgctgg tggggttggc ggtggccacg gaagtgttca tagctcggac tccctgcgca
     1141 gcagcctgcg ctgccactgt tcaaactcgc ggtcgacgtc cagcagcttg cggttggacg
     1201 acagcgccat gctggcctgc gcgcccgcgc cgcgcggcgc cagccacacc cacaggcgtc
     1261 gctcgcgcac atacagcatg gcgaaaacgc ccaggatcag caacaggcag cccagataga
     1321 ccacattctt gcctggggct cgcgccacct ggaacacgct ggcctgcacc tgctcgaaac
     1381 cggtcagctc gaacgccacc ggcgccgggt acagcggcgc gtccgacagc gccagcaccg
     1441 cctgcgtcat gaagcgccgg ctggcttcgg actcggtcag cggcggcttg ccctgggcct
     1501 ggcgcgccag gtcgttgagc tccagcaggc tgccgttgag gatgcgcagc agcacctcgg
     1561 cggcgcgttc gcgctcgctt tcgggcaccg ccgtctcgat gaagtccgac accgcctgca
     1621 gaccgccggt ggtgcggcca ttgacggctt cggcgccggc aaacagcccc agcgcgcggc
     1681 tggccgactg cgccagctgc tccaccagtt cggggcgctg cgggtcgacc gccttgcgca
     1741 cgtagcggcg caccgcctgc tcgcgctggg ccgcgtcctg cagcgcgccg cgcaggcgca
     1801 cgaagccatc gatggcgccg tcctcgtcgg ccggcacgcg caggtagcga aagccctcgg
     1861 cctggctggc gcgcacgccc agcaggaaca ccgcgccacc gtcgcccatg tccaccggca
     1921 gcatgtagtt gtggaattcg ttggcctggc cggccgcgtc gcgcagcttg taggtgatgc
     1981 tggggccgat gttgcgcagc tcgcgctgcg tcaccgtctt gtcggccgcg cccagccgct
     2041 gcgtcagcgc ctgacccagg tcgaccttgc gcacgtcggc gcccgagccc ctggcgcctt
     2101 cgctgaggtt ttcgacgttg atcacgcgca gcgccgtgta ttccagcgtc agcttgtcgc
     2161 tgccgttggt gatctgggcg ttgccaccga tggtgccctc gacctcgaac gccttggccg
     2221 cgccgttcat gggcacggcg cgcagcttga cgcgcgagcc gccgtcgtcg aagctgctct
     2281 ggtagatctg cacgcccttg tagctggccg ggtggttgac ctcgacgcgc gccggctgcg
     2341 tctggccggt ggccttgtcg tggatcacga tctcgctggc gaacagcttg ggcatgccgg
     2401 tggcgtagta ctcgacgatg aacttcttca gctcgaccgc gaacggcagc tcctgcagca
     2461 gcacgccgtc ggactggctg aggatggcgg tgctcgactg cgtgccctcc gacaccacca
     2521 ggttgccgcg gaacgccggg ttggtttcgg gcaggcggtg ttcgggccgc acctgggaca
     2581 ccaggccgcc gccgctgtag acgctcttgc cgcccagcca ggtcatggcg cgcacgaaca
     2641 ggtcgccgtc gaacagcccg cccaggcaga tcagcacgat ggcgctgtgc gccgcgatgt
     2701 agccgatctt gttggcggcg ccggccttgg ccgccaccat ccagccctgg ccttcgggcg
     2761 tgtcgcgcgc ctgcaggcgc accttccaac cgccgccggc cagttgctgg ccgatgcgcc
     2821 gcgccgccac ctcggggctt tcgtccagct cgccctcggc cttgtggtga aaggcctgca
     2881 ggctctggtg gcggatgttt tccttgtagc tgcgaatgtc ggccagaaac ttgggcgtgt
     2941 tgcgcagcac gcacaggctg gtgctgacga ccagaaaggc caggatcagc aggaaccacc
     3001 aggcgctgta gacggcgttg agctggagct tgaggaacag ctcggcccag aacggcccga
     3061 actggttgac gtagttgggc gccggctcgt gctgcttgat gacggtgccg atcaccgagg
     3121 cgatgcagat gacggtgagc agcgcgatgg caaagcgcat cgacgacagc aactcgacgc
     3181 tggcgcgcca ggcggccgag ccatgtcggg gttggtagcc gtgggtgctg tgggaggcgg
     3241 tcatgcaagg ggtggcggcg gcggccatgc cgtccggcgt cgggccatca gggctggagt
     3301 cgatctgggg cgccgggttc caccgacacg gtgattatct tgacttgggt cacgatcggc
     3361 ggccaggaaa acaaaagccg cgcccggggc atggacgcgg cttggcgcac gggcaggccg
     3421 ggccggcgct cagcgcaggc cggcgatgta gtcggacacg gccttgatct cgcggtcgtt
     3481 catcttggcg gccacgccct gcatgacggc gttgttggcg cgcgtgccgt cgcggaaggc
     3541 caccatctgc ttgaccgtgt agtcggtgtg ctgccccgac aggcgcgggt attgcgacgg
     3601 gatgccggcg ccattggggc tgtggcagcc ggcgcaggcc gggatgtggc ggtccggaat
     3661 gccgccgcgg aagatgcgct cgccggtcag caccaggtcc ttgtcgctgg cggcgccggg
     3721 cttggccttc tgcgcggcca gccaccacga aatgttgcgc gcgtcgtcct cgctcagcat
     3781 ggcggcaaag ccctgcatca ccgggtcctt gcgcgcgccg ctcttgaatt ccagcagctg
     3841 cttgagggtg tattccgggt gctgctgggc caggctgggc tgcagcggca cggtggaatt
     3901 gccgtcggcc gcatggcagg ccacgcacac ggtggcgtaa ctggccgcgc ccttggccag
     3961 atcgaccttg ggcgccgcgg gtgcgtcggc cgccgaggcg gtggcggcca gcagggaggc
     4021 ggtaagcagg gcggcaagcg atttcatggg acgcatcgtc ttgtgggaag gggttgtcac
     4081 ggggcgtgaa tccgggcgat tctacaatgc ccccacccgc cattccttga tccaggccgc
     4141 cccggcgccc acatggcgcg gcccctcgca tgaccgcccg ccctgcccca gcccccgccg
     4201 ccgccgccgc ccgccccgat gctgccaccc cagccgcggc ggcgcgcgag gcgctgggct
     4261 ggctgcacac ggcgcgcttt ctgaccaccg ccgcccagct gcaccaactg ccggcgctgg
     4321 ccgtgccgga aatcgccttc gtcggccgct ccaacgccgg caagtcgacc gccatcaaca
     4381 ccctgacgca gcagacccag ctggccttcg cctcgcgcaa gccggggcgc acccagcaca
     4441 tcaacctgtt cgcgttgggc cgccagggcg gcaccgacgc cgtgctggcc gatctgccgg
     4501 gctacggcta cgccgccgtg ccgctggccg acaagcagcg ctggcagcgc gtgatggcca
     4561 actacctggt gacgcgcgag aacctggccg ccgtggtcat gctgtgcgac ccgcgccacg
     4621 ggctgcagga gctcgacgaa atcctgctcg aagtgatccg cccgcgcgtc gagcagggcc
     4681 tgccgttttt gatcctgctg accaaggccg acaagctcaa ccgcagcgaa caggccaggg
     4741 cgctgcagat cgcccgcctg caggccggcg gcggcagcgt gatgctgttt tccgccccca
     4801 agcgccaggg cgtggaagac gccgcgacat ggctgcgcgc ggtggtggac gccgccgcca
     4861 acccatccga cgccgcctga ggcgacggct gcgccgtcac cggtcggacg gccgagcctt
     4921 gcgccaccgg caccgcgcgc tctaccatgg cgccgacatt cgggcaaaac cggcaccaac
     4981 gcatgattgg cgcgcgccag cagctatcaa acaaggagcg ctcaagatga tcgaaacctg
     5041 gctcgccgaa ctgccccacg gcatcaccct gagctgccgc ggcgccggcg cacgcgggcg
     5101 gccgctgatg ctgttgctgc acggctttcc agaggccgcc ttcatctggg atgcgctgat
     5161 ggcgcatttc gcgcagcccg aacacggcgg ctttcgctgc gtcgcgccca acctgcgcgg
     5221 cttcgagcgg tccagcgcgc cggcggaggt ggccgcctac aagccggcgc tgatggtgca
     5281 ggacgtgctg gcgctggccg ccgccgaggg ggtggacggc cgcatccacg ccctggtggc
     5341 gcacgactgg gggggcgcct ttgcctgggc ggcggcgcat gcgcacccgg cccaggtcga
     5401 ccacctggtg atcatcaact cgccccaccc cgccaccttc gcgcgcgagt tgcagcgcag
     5461 cccggagcag cagcaggcca gcgcctacat gaacttcctg gcccggcccg acgccgaggc
     5521 gctgctggcc gaggacgact accgccgcct gtggccgttc ttcacctcga tgggcgcggg
     5581 cccggagcgc ttcggctggc tgaccgaggc cgtgcgccag cagtaccgcg acgtctggcg
     5641 ccatggcctg accggcgcct gcaacctgta ccgcgtcaca ccactcaagc ccccggcgcc
     5701 ggggcagcgc ggcgtcaccg acatcccgca gccgccgccc gagcgcgtgc gcgtggaggt
     5761 gccgaccctg gtgctgtggg cgctggatga caccgcgctg cgtcccggcc tgctggacgg
     5821 cctggacgcc cacgtgccgc gcctgaccgt gcaacggatg cccggcgcca cgcactgggt
     5881 ggtgcacgag cagccgcagc aagtggccgc gcacatcggc gcctttgtta ctacgaaata
     5941 gatagctgct cgcgcagcct ggaagcgcgc caaatgccta tttggcgtag gacattcgcc
     6001 catcgccatc caacgaccgc ggagcaggcc aggccagcga acgccgtggc cggacctgcg
     6061 ccggccactg gcgttgtccc cctccgggag atggcgccga aggcgactca gggggtgcgt
     6121 ccatacaccg acggctcgcc cgccggccgc gtcttgaagc gcttgtgcac ccagtagtac
     6181 tgctcgggca tggtgcggat ccagtcttcc agccggcggt tcatcagcgc ggtgtcggcc
     6241 accgggtcgt cgcccgggta gccgctccag gccgggtgca cctgcacctc gtagccgccg
     6301 ggcgtcatgc gggtgacggt gggcaccacg cgcgcgcgcc ccagccgcgc cagccgcggc
     6361 agcgacggca cggtggccgc cggcacgccg aagaagggca cgaagacgga ttcctccggc
     6421 cccaggtcca tgtcgggcag caggtacagg tagcggccct cgcgcagcga cttgacgacc
     6481 ggcttgacac cgtcgcgccg gttgatcagc accacgtcgc cgaagcgctc gcgcccggcg
     6541 cgcacccagg cgtcggtggc ggcgcccgac tgcgggctgt agatgctgcc gcccttggcc
     6601 accgcctgct gcatgatggc cgcgccgccg gcgtccatgc cgtagaagtg cggcgcaaac
     6661 agcaccgccg cctcgggccg gcgcagctcg tccaccgcgc cggtcaggcg gatgcgctgg
     6721 cgcaccacgg cgtcgggcgc gtgccagacc caggcgcggt cgatgaagga ctgggcaaag
     6781 gcgatgaagc tgcggcgcgc cacgtccatg cgctcggcct cgctccaggc gggaaaggcc
     6841 agccgcaggt tgaccagcgc gatgtggcgc cgccgccgcg ccagtgcgaa cagcagccgc
     6901 cccagcacgt cgcccagcgc gcgcagccag ggcagcggca gcggcgccag cgcgcgcatc
     6961 agccagacgc cggggtgggt cgtcacgccg gctgctcccg cggttgcttg tagcggccat
     7021 agccccacag gtactgcgtc gggcattccc ggatcaggcg ctccatctcg cggttgatct
     7081 gcgccaccgc gggcgccagc tcgtccgcca gcggctcggc cagggcgcgg aagtgcaggt
     7141 cgtagccctg cccgcgcggc cgccgttcgc cccacaccag cagcggcacg gcgccggtct
     7201 gctggatcag ccgcgccgcc agcgtcatgg tgtaggccgg ctggccaaaa aacgggctcc
     7261 acagaccctg cccctcgggc ggcacctggt cgggcagcag gccgacggcg cgcccggcgc
     7321 gcagcgcctt gatcatctgg cgcacgccgg ccagggtggt gggcgcggtg tccagcccct
     7381 cgcgccggcg cgccgtctcc accacctgcg ccagcgccgc ctggcgcgcc ggacggtaca
     7441 gcacggtgat ggggccgtag cgcgcgtggt agcgctcggc caggccctgg gcggtgatct
     7501 cgaagcagcc caggtgcggc gtcaggaaca ccacgccacg gccggcggcg taggcggcgt
     7561 ccaccagttc ggtgccgtcc caacgcatgg gcaccggcgg gccgaaccac aggcgcggcg
     7621 tctccagcgc catgcggccg gcgtgcgcca ccgccgggcg cacctgggca aagccgtagc
     7681 cggccagcgc cgcgttgtcg cgcagccggc gccggtaggt gggcgacgcc agccagctca
     7741 gccagcccag cgccgcgccc agcgcgtgca gcagcgacag cggccagcgc gccgccaggc
     7801 ggtacagggc aagcatggtg gatccagtaa aatcgcgccg tcgccgagtt atggaactac
     7861 ttgcagggcg acgttaaaca agtgctgcta aagcgttcgc cagggctccc gacagtttgg
     7921 caacgcaacg ttcaaacccg tggagtttat tcaaatggaa ctggcgaaca gctttctctt
     7981 cacctccgaa tcggtttccg agggccaccc cgacaaggtc gccgaccaga tctccgacgc
     8041 catcctcgac gccatcctgg cccaggacga gcgctcgcgc gtggccgccg aaacgctgac
     8101 caacaccggc ctggtggtgc tggccggcga aatcaccacc aacgcgcacg tcgactacat
     8161 ccaggtggcg cgcgacacca tcaagcgcat cggctacgac aacaccgagt acggcatcga
     8221 ctacaagggc tgcgccgtgc tcgtcgccta cgacaagcag agccacgaca tcgcgcaggg
     8281 cgtggaccac gccagcgacg accacctcaa caccggcgcc ggcgaccagg gcctgatgtt
     8341 cggctacgcc tgcgacgaga ccgacac
//
LOCUS       DAOQTZ010000110         8334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_9490_length_8334_cov_14.453557, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000110 DAOQTZ010000000
VERSION     DAOQTZ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9490_length_8334_cov_14.453557"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1171
                     /locus_tag="PKN51_11400"
                     /pseudo
     CDS             1..1171
                     /locus_tag="PKN51_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592726.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
     gene            1358..2248
                     /locus_tag="PKN51_11405"
     CDS             1358..2248
                     /locus_tag="PKN51_11405"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNT85747.1"
                     /translation="MTSTTRSKHALPSRASTRMRTPWLLASAGAALLALTLTLRQEAV
                     ARQTVDLGLPPPLMLAALLLLLALGFGILAWPARRRAGVGVRRPLASPDWSRLTQMAN
                     WFFDAHGLVREGATRPWAAGQTLLLHNESHRFLVQTKLWRARRVDAAAVQRLSREMAR
                     RGADRGMLLCAGDVFTAAARQLARRSGILLLEPQQLSSRPSAAPRPDARSQAATLSTL
                     PPPAEPAPVRAEAPTVLLSLDDAAPPRFEFMPTVPMSKAERLAAVAPVLRPDHEVARG
                     LCEFMPTVPSALSPSAATQA"
     gene            complement(2340..4679)
                     /locus_tag="PKN51_11410"
     CDS             complement(2340..4679)
                     /locus_tag="PKN51_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT85748.1"
                     /translation="MTKRTTTTVREPAGMLATPGLAAAPVMDLMCSHFVLTLAAQQGS
                     KFNVRRDINGLMALAGRHLMWPQAVLARLREFLARRCKDNEFWRGHERLSDAEFLERH
                     GAWRGPYEEGTLFFYLDEYAKDAPKDLLALLAATGQWLRHALKKQSTLVEKNIDALAN
                     LLQLNKAERALLLYGTLARYQRDLRSLLVEFKVNNAPEAYAAIGDLAGVSAAEVGEAL
                     RAGGRLERIGLVENLISEHSITDLADLMKVSEKLPPVLMREYRDRAELMAVFTRPASK
                     SSLKASDFSFVGDDVQVLIQLLRKAQQTQAAGVNVLLYGPPGTGKTELAKVVAREAGL
                     DLFEVEYADRDGNSLSGRDRYRSMQIAQVFLKGAHEAALLFDEVEDVFPPISVDAAGL
                     MARAEQQALSAAVSHSVNGKAWVNQILESNPVPTLWVTNRIEQIDPAFRRRFAYHLEL
                     KSPPPGAREQLVRRALDGVEVSEALVVRLTERKGLTPAQIRTAVRFADLAHADDARGG
                     AAFEALIERQLKNADAALGRSGESAPAKGRRSVTTYDLGMLNVESRFEVPRIVEALKA
                     RGHGALCFYGAPGTGKTALGEYIAEQMGKPLIVRQASDLVSKFVGETEQNMAAMFKEA
                     SEEKAVLLLDEADSFLLDRRGAQRSYEVTEVNEMLQQMERHDGVFICTTNLLDRIDQA
                     ALRRFTFKIRFKPLTAEQREHMFVTEALDGQADALTDDARRRLARLEQLCPGDFAAVK
                     RQVDILASRLTPEEFLEQLESEHRIKPEVREQRSMGFLH"
     gene            4867..6417
                     /locus_tag="PKN51_11415"
     CDS             4867..6417
                     /locus_tag="PKN51_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNT85749.1"
                     /translation="MADWNTMWAGLRGTLGIHVLQLLGALLIVIVGWFLAALVKAGAR
                     KGLRLLRLNERFAGTSGQRADLEGAIALALFWLVMLLTLGAMFGALELSQVSGSFAAL
                     NLQLFEYAPRVLGAALLALLAWLVAALVRGLARKLLDRTTLDDKLSEHAHIAPISENL
                     SNALFWLVVLLFVPAILGTLQMDGLLLPLRDMTARAVDILPDIVAAVVIGGVGWVVSM
                     VLRNLTSNLLRSAGFDQLGSKLGLAAGVQLSRLAGLLVFVLVFVPALIAALDALRIEA
                     ISRPATDMLGVLLDAVPRIMAAGLILLLTWLVATLATRLLSSLLASLGLDTLPARLGL
                     QQAFARTPPSQLIGRVGLLFAMLFAVVEASNQLGFQRLADLVTTFIEFAGDVLLGSLI
                     LIVGFMLANAAYEAISRASGRHTPLARLARLAILGIVLAMGLRAMGIADDIVNLAFGL
                     TLGAIAVAVALAFGLGGREAAARLAAHWVDRWTREQAADEAAATPPPSPPPPPEPEPE
                     PAPPGDRP"
     gene            6514..6648
                     /locus_tag="PKN51_11420"
     CDS             6514..6648
                     /locus_tag="PKN51_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85750.1"
                     /translation="MSASTEHLTSEDPVESVTRSIPVVIPLAGAVLIFLLAFIAVTVA
                     "
     gene            6914..>8334
                     /locus_tag="PKN51_11425"
     CDS             6914..>8334
                     /locus_tag="PKN51_11425"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNT85751.1"
                     /translation="MNAPLTPGMIQDLHVPPYVQHARLIAWVGDMVALCKPDRVHWCD
                     GSQAEYERLCQQLVEAGTLKKLNPAKRPGSFLACSDPSDVARVEDRTFICSERQQDAG
                     PTNNWEPPAEMRAKLEPLFDGCMRGRTMYVIPFSMGPLGSPIAHIGVELSDSAYVAVN
                     MKLMTRMGRGAIEVLGDNGDFVPCVHTVGAPLAPGQQDQTAWPCNPEVKYIVHYPETR
                     EIWSYGSGYGGNALLGKKCFALRIASKMGQEQGWLAEHMLILGVTSPEGKKYHVAAAF
                     PSACGKTNFSMLVPPAGFEGWKVTTIGDDIAWIKPGPDGRLYAINPEAGYFGVAPGTN
                     LHTNPNCMHSLDRDVIFTNVALTDDGDVWWEGMEKDTGVLPEHLIDWQGKDWTPADGQ
                     AGRKAAHPNARFTVAATNNPVLDAAWDDPAGVPIDAFIFGGRRSTTVPLVTEARDWQE
                     GVYMAATMGSETTAAAFGAQGVVR"
BASE COUNT         1193 a         2974 c         2863 g         1304 t
ORIGIN      
        1 cagcagcacg agggcgaggc cggcatcgtc tactgccagt cgcgcaagcg ggtggaagaa
       61 ctcgcggcca cgctgtgcca gcacggcatc gccgcgctgc cctaccacgc cggcctgccg
      121 gccgatgagc ggaagcagaa ccaggagcgc tttcagcgcg aggacggggt ggtgatgtgc
      181 gccaccatcg ccttcggcat gggcatcgac aagcccgacg tgcgctttgt cgcccatgtc
      241 gatctgccca agaacatcga gggctattac caggagaccg gccgcgccgg gcgcgacggc
      301 gcgccggccg acgcctggat ggcctacggc ctgcaggacg tggtcaacca gcgccgcatg
      361 atcgacgaaa gcccggccga ggaagacttc aagcaggtga tgcgcggcaa gctcgacgcg
      421 ctgctggcgc tggccgaggc caccgactgc cggcgtgcgc ggctgctggc ctactttggc
      481 gagcagatgg tgtcccggcc gcgtgaggat actattgatt cgatagctat tcgcgcaaca
      541 ccagcaagcg ccagcggcca aaatggctcc tatccgcagc ccctcgccta ctgcgggggc
      601 tgcgacaact gcctgcagcc gccccaggtc tgggacgcca ccgactgcgc gcgcaagctg
      661 ctgtccaccg tctatcgcgt gcagcagatg agcggcatcg gctttggtgc cggccacctg
      721 atggacatcc tgcgcggcaa ggccaccgac aaggtgcgcc agttcggtca cgagcagctc
      781 agcaccttcg gattgggcgc cgagttcagc gaggcgcagc tgcgcgccgt gctgcgccag
      841 ctgatcgcca gcggcgcgct gcacgtcgat gccgagcgct accacacgct gcagctgcaa
      901 gacggcgcgc ggcgccacgc ccgcgcccgc cgcggccagc ctggacgccg ccgcccagca
      961 gcgctttgcc gacctcaagg cctggcgcgg cgaagtcgcg cgcgagcaca acctgcccgc
     1021 ctacgtgatc tttcacgacg ccaccctggc cgccatcgcc gagcgcgcac cgcagtcgct
     1081 ggccgagctg cagggcatca gcggcatcgg cgccagcaag ctggagcgct acggcgacga
     1141 ggtgctgcgg gtggcgcgcg gggccggttg agcgtcgcct gccggccacg acccggcggc
     1201 gcccgccgcg ccgcgactgc gcactattgc acgccgtcgc cccgtggcgg ggtgcggcgc
     1261 cggcgccgac gcgcggcgcg tgaactgtga cacagacatc agcgccccgg cgcgccgaca
     1321 atgccgctgg gcacccgctg cccgcaggga gaatcacatg acatccacca cgcggagcaa
     1381 gcacgctctg ccctcgcggg cctcgacccg catgcgcacg ccctggttgc tggccagcgc
     1441 cggcgcggcg ctgctggcgc tgacgctgac gctgcggcag gaggcggtgg cgcgccagac
     1501 cgtcgatctg ggcctgccgc cgcccttgat gctggcggcg ctgctgctgc tgctggcgct
     1561 cgggttcggc attctggcct ggccggcgcg ccggcgcgcc ggcgtgggcg tgcggcgacc
     1621 gctggcgtca ccggactggt cgcggctgac ccagatggcc aactggttct tcgatgccca
     1681 tggcctggtg cgcgagggcg ccaccaggcc ctgggcggct ggccaaaccc tgctgctgca
     1741 caacgaatcc caccgctttc tggtgcagac caagctgtgg cgggcgcgcc gggtcgacgc
     1801 cgctgcggtg cagcggctgt cgcgcgaaat ggcgcgccgc ggggccgatc ggggcatgct
     1861 gctgtgcgcc ggcgacgtgt tcacggcggc ggcgcggcag cttgcccgcc gcagcggcat
     1921 cctgctgctc gagccgcagc agctgtccag ccgtccgagt gccgcaccgc ggcccgacgc
     1981 caggtcgcag gccgcgacac tcagcaccct gcccccgccc gccgagcccg cgcccgtgcg
     2041 cgccgaagca cccaccgtcc tgctgtcgct cgatgacgcg gcgccgcccc ggttcgaatt
     2101 catgcccacc gtgccgatga gcaaggccga gcgcctggcc gccgtggctc cggttctgcg
     2161 ccccgaccac gaggtggcgc gcgggctgtg cgaattcatg ccgaccgtgc cgagcgcgct
     2221 gtcgccgtcc gccgcgacgc aggcttgatt cacgaaccgg cgcagccggt gtcatcggtc
     2281 gcgttcttgg gcgcgaagcc gtttctaggt tcaaacatca agtttccgaa gcaacaggct
     2341 cagtgcagaa agcccatcga gcgctgctcg cgcacctcgg gcttgatgcg gtgctccgat
     2401 tcgagctgct cgagaaattc ctccggcgtc aggcggctgg ccaggatgtc cacctgacgc
     2461 ttgacggcgg cgaagtcgcc cgggcacagc tgctccagcc gggccaggcg gcggcgcgcg
     2521 tcgtcggtca gcgcgtcggc ctggccgtcc agcgcctcgg tgacgaacat gtgctcgcgc
     2581 tgctcggcgg tgagcggctt gaagcggatc ttgaaggtga agcgccgcag cgccgcctgg
     2641 tcgatgcgat cgagcaggtt ggtggtgcag atgaacacgc cgtcgtggcg ctccatctgc
     2701 tgcagcatct cgttgacctc ggtgacttcg tagctgcgct gggcgccgcg tcggtccagc
     2761 agaaagctgt cggcttcgtc cagcagcagc accgcctttt cctcgctggc ttccttgaac
     2821 atggcggcca tgttctgctc ggtctcgccg acgaacttgc tcaccaggtc gctggcctgc
     2881 ctgacgatca gcggcttgcc catctgctcg gcaatgtatt cacccagcgc ggtcttgccg
     2941 gtgcccggtg cgccgtagaa gcacagcgcg ccatggccgc gcgccttcag cgcctcgacg
     3001 atgcgcggca cctcgaagcg gctttcgacg ttcagcatgc ccaggtcgta ggtggtgacg
     3061 ctgcgccgcc ccttggccgg cgcgctctcg ccgctgcgcc ccagcgcggc gtcggcgttc
     3121 ttcagctggc gctcgatcag cgcctcgaag gcggcgccgc cgcgggcgtc gtcggcatgc
     3181 gccaaatcgg caaagcgcac cgcggtgcgg atctgcgccg gcgtcaggcc cttgcgctcg
     3241 gtcagccgca ccaccagggc ctcgctgacc tcgaccccgt cgagcgcccg gcgcaccagc
     3301 tgctcacgcg cgccgggcgg cggtgacttc agctccaggt ggtaggcaaa gcggcgccgg
     3361 aacgccgggt cgatctgctc gatgcggttg gtcacccaca gcgtgggcac cgggttggat
     3421 tcgaggatct gattgaccca ggccttgccg ttgacactgt ggctcaccgc cgccgacagc
     3481 gcctgctgct cggcgcgcgc catcaggccg gcggcgtcga cgctgatggg cggaaacacg
     3541 tcctccacct cgtcgaacag cagcgccgct tcgtgcgcgc ccttgaggaa aacctgggcg
     3601 atctgcatcg agcggtagcg gtcgcgcccg gacaggctgt tgccgtcgcg gtcggcgtat
     3661 tcgacctcga acaggtccag cccggcctcg cgcgccacca ccttggcgag ctccgtcttg
     3721 ccggtgcccg gcgggccgta cagcagcacg ttgacgccgg cggcctgggt ctgctgggcc
     3781 ttgcgcagca gctggatcag cacctgcacg tcgtcgccga cgaagctgaa atcgctcgcc
     3841 ttcaggctgc tcttgctggc cgggcgggtg aacaccgcca tcagctcggc gcggtcgcgg
     3901 tattcgcgca tcagcaccgg tggcagcttt tcgctcacct tcatcaggtc ggccaggtcg
     3961 gtgatgctgt gctcggaaat caggttctcg accaggccga tgcgctccag ccgcccgccg
     4021 gcgcgcagcg cctcgcccac ctcggcggcg ctgacgccgg ccagatcgcc gatggcggca
     4081 taggcctcgg gcgcgttgtt gaccttgaac tccaccagca ggctgcgcag gtcacgctgg
     4141 tagcgcgcca gcgtgccgta cagcagcagg gcgcgttcgg ccttgttcag ttgcagcagg
     4201 ttggccagcg cgtcgatgtt cttttcgacc agggtggact gcttcttgag ggcatggcgc
     4261 agccactggc cggtggcggc cagcagcgcc agcaggtcct tgggggcgtc cttggcgtat
     4321 tcgtccaggt agaagaacag cgtgccttcc tcgtaggggc cacgccaggc gccgtggcgc
     4381 tccaggaatt cggcgtccga cagccgctcg tggccgcgcc agaattcgtt gtccttgcag
     4441 cgccgggcca ggaactcgcg cagccgcgcc agcaccgcct ggggccacat caggtggcgg
     4501 ccggccagcg ccatcaggcc gttgatgtcg cgccgcacgt tgaacttgct gccctgctgg
     4561 gccgccagcg tcagcacgaa gtgcgaacac atcagatcca tcaccggcgc agccgccaga
     4621 ccgggcgtgg ccagcatgcc cgcgggttcg cgcaccgtcg tcgtcgtccg tttcgtcatc
     4681 agcgacctcc ctgaaggttg agcgccagat caagcgccac cataacgcaa tccacgcagg
     4741 cgcggaagac gccggccggt cacgtccgcg gcgccggtgg cggcatttcg tcacgctcgg
     4801 cggccggccg gcgctggcgg cgcggcaggg ggtcacaata ggcggcaaca caggagaagc
     4861 atcgacatgg cggactggaa cacgatgtgg gcgggcctgc gcggcacgct ggggatccac
     4921 gtgctgcaac tgctgggggc cttgctgatc gtcatcgtcg gctggttcct ggcggcgctg
     4981 gtgaaggcgg gggcgcgcaa gggcctgcgc ctgctgcgcc tgaacgagcg ctttgccggc
     5041 acctcgggcc agcgcgccga cctggagggc gcgatcgcgc tggcgctgtt ctggctggtg
     5101 atgctgctga cgctgggggc gatgttcggt gcgctcgagc tgtcgcaggt gtcgggctcg
     5161 ttcgcggcgc tcaacctgca actgttcgaa tatgcgccgc gcgtgctggg ggcggcgctg
     5221 ctggccttgc tggcctggct ggtggcggcg ctggtgcgcg gtctggcgcg caagctgctg
     5281 gaccgcacca cgctggacga caagctgtcc gagcacgccc acatcgcgcc gatcagcgaa
     5341 aacctgtcca acgccctgtt ctggctggtg gtgctgctgt tcgtgccggc catcctgggc
     5401 acgctgcaga tggacggcct gctgttgccg ctgcgcgaca tgacggcgcg ggcggtcgac
     5461 atcctgcccg acatcgtggc ggcggtggtg atcggtggcg tcggctgggt ggtgtcgatg
     5521 gtgctgcgca acctgaccag caacctgctg cgctcggccg gtttcgacca gctgggcagc
     5581 aagctggggc tggccgccgg cgtgcagctg tcgcgcctgg ccgggctgct ggtgttcgtg
     5641 ctggtgttcg tgccggcgct gatcgccgcg ctggacgcgc tgcgcatcga ggccatctcg
     5701 cgccccgcca ccgacatgct gggggtgctg ctggacgccg tgccgcgcat catggccgcc
     5761 gggctgatcc tgctgctgac ttggctggtg gccacgctgg ccacgcgcct gctgtccagc
     5821 ctgctggcca gcctcggtct ggacaccctg ccggcgcggc tcggtctgca gcaggccttt
     5881 gcgcgcacgc cgccgtcgca gctgatcggt cgcgtcggcc tgctgtttgc catgctgttc
     5941 gcggtggtcg aggcgagcaa ccagctgggc tttcagcgcc tggccgacct ggtgacgacc
     6001 ttcatcgaat tcgccggcga cgtgctgctg ggctcgctga tcctgatcgt cggcttcatg
     6061 ctggccaacg ccgcctacga agcgatttcg cgcgccagcg gccgccacac gccgctggcc
     6121 cggctggcgc gtctggccat cctgggcatc gtgctggcca tgggcctgcg cgccatgggc
     6181 attgccgacg acatcgtcaa cctggctttc ggcctgacgc tgggcgccat cgcggtggcc
     6241 gtggcgctgg ccttcgggct gggcgggcgc gaagccgccg cccgtctggc ggcgcactgg
     6301 gtcgatcgct ggacccgaga gcaggccgcc gacgaagcgg cggccacccc gccgccgtcg
     6361 ccaccaccgc cacccgagcc cgagcccgag cccgcgccgc ccggcgatcg gccctgaggg
     6421 cgcccccggc gccgggtttg accccatgca agccgcaggt ggcgcgcata gaatgccccg
     6481 ctatccccga ttccgaaccc aaaggccatt cccatgtctg ccagcaccga acatctgacc
     6541 tccgaagacc cggtggaatc cgtgacccgg agcatccccg tggtcatccc gctggccggc
     6601 gcggtgctga tcttcctgct ggccttcatc gccgtcaccg tcgcctgagt ttcagcgcca
     6661 aatcggcctt caagtcccac caggaaagcg ccagcagcta ttgaaatcat agcttgcggc
     6721 gcgccctccc ggcccacggc agggccgtca ggatcgcgtg gggcggattt cataaaatca
     6781 tccccctttg cccgtggctg caaaggggcg tgccgtcgcc gcagcgtccg tgcgggtcga
     6841 ccagggttcg agtcggcttt cccaaggcct ggcgccttcg gaaagagcga ttttcatttc
     6901 aggagctttg tcgatgaacg cacccctgac ccccgggatg atccaggatc tccacgtccc
     6961 gccgtacgtc cagcatgccc gcctgatcgc ctgggtgggc gacatggtgg ccctgtgcaa
     7021 gcccgaccgc gtgcactggt gcgacggcag ccaggccgag tacgagcggc tgtgccagca
     7081 gctggtcgag gccggcaccc tcaagaagct caaccccgcc aagcgcccgg ggtcgtttct
     7141 ggcctgctcc gacccgtccg acgtggcgcg ggtggaagac cgcaccttca tctgctccga
     7201 aaggcagcag gacgccggcc ccaccaacaa ctgggagccg ccggccgaaa tgcgcgccaa
     7261 gctggagccg ctgttcgacg gctgcatgcg tgggcgcacc atgtacgtca tccccttcag
     7321 catgggcccg ctgggcagcc cgatcgccca catcggggtc gagctgtccg acagcgccta
     7381 cgtggccgtc aacatgaagc tgatgacccg catgggtcgc ggcgccatcg aggtgctggg
     7441 cgacaacggc gacttcgtgc cctgcgtgca cacggtgggc gcgccgctgg cgccgggcca
     7501 gcaggaccag accgcctggc cctgcaaccc cgaggtcaag tacatcgtcc actaccccga
     7561 aacgcgcgaa atctggtcct acggctcggg ctacggcggc aacgcgctgc tgggcaagaa
     7621 gtgctttgcc ctgcgcatcg cctccaagat gggccaggag cagggctggc tggccgagca
     7681 catgctgatc ctgggcgtga ccagcccgga aggcaagaag taccacgtgg ccgccgcctt
     7741 tcccagcgcc tgcggcaaga ccaacttctc gatgctggtg ccgccggccg gctttgaagg
     7801 ctggaaggtc accaccatcg gcgacgacat cgcctggatc aagcccggcc cggacggccg
     7861 gctgtacgcc atcaaccccg aggccggcta cttcggcgtg gcgcccggca ccaacctgca
     7921 caccaacccc aactgcatgc acagtctgga ccgcgacgtg atcttcacca acgtcgcgct
     7981 caccgacgac ggcgacgtct ggtgggaagg catggaaaag gacaccggcg tgctgcccga
     8041 gcacttgatc gactggcagg gcaaggactg gaccccggcc gacggccagg ccggccgcaa
     8101 ggcggcgcac ccgaacgcgc gcttcaccgt ggccgccacc aacaacccgg tgctcgacgc
     8161 cgcctgggac gacccggccg gcgtgccgat cgacgccttc atcttcggcg gccgccgctc
     8221 caccaccgtg ccgctggtga ccgaggcgcg cgactggcag gagggcgtgt acatggccgc
     8281 caccatgggc tccgagacca ccgccgccgc ctttggcgcc cagggcgtgg tgcg
//
LOCUS       DAOQTZ010000111         8251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_9606_length_8251_cov_14.206198, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000111 DAOQTZ010000000
VERSION     DAOQTZ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9606_length_8251_cov_14.206198"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /gene="rrf"
                     /locus_tag="PKN51_11430"
     rRNA            <1..110
                     /gene="rrf"
                     /locus_tag="PKN51_11430"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            230..1693
                     /gene="tldD"
                     /locus_tag="PKN51_11435"
     CDS             230..1693
                     /gene="tldD"
                     /locus_tag="PKN51_11435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNT85752.1"
                     /translation="MNTRNIKHHHLATARALLLDPYGLSERELDATLATIARHRVDDA
                     DLFFQYTRTEGWSLEEGIVKTGSFSIDQGVGVRAVTGERTAFAYSDDLSPGSLQDAAC
                     TVRAISAAAGVGRVDRRRRRASGRYRPLYAPIDPIATLDSGAKVALLEMVEQRARARD
                     PRVVQVMAGLASEYDVVLVARLDGTLAADVRPLVRLSVTVVAEQGGRREIGSSGGGGR
                     FGLTHFDASRVAQYVDEAVAAALTNLESRPAPAGEMTVVLGPGWPGVLLHEAVGHGLE
                     GDFNRKGSSAFSGRIGQRVAAKGVTVLDDGTLADRRGSLNVDDEGHVTQRNVLIEDGI
                     LRSYLQDTLNARLMKVKPTGNGRRESYAHIPMPRMTNTYMLGGDRDPREIVASIRKGL
                     YATNFGGGQVDITSGKFVFSASEAYWVENGRIQYPVKGATLIGSGPESLKKISMIGND
                     MRLDSGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTG"
     gene            complement(1768..2547)
                     /locus_tag="PKN51_11440"
     CDS             complement(1768..2547)
                     /locus_tag="PKN51_11440"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNT85753.1"
                     /translation="MTFLAIDVGNTRLKWTLYERPAVGARVLAQGVEFLEQLDRLAEG
                     PWADLPEPSRMLGCIVAGQAVKHRVQEQMEHWGVEPRWVVASESEAGLVNGYDFPSRL
                     GADRWVAMVGARHHMLQRGPARPVLLVMVGTAVTVEAIDAQGRFLGGLIIPGHGIMLR
                     ALESGTAGLHVPTGDVVEFPTNTSDALTSGGTFAIAGACERMFQHLFKRCAAEPLCLM
                     TGGAGWKMLPAMSRPYELIESMIFDGLLVIAEAREALNGGL"
     gene            2662..3144
                     /gene="lysM"
                     /locus_tag="PKN51_11445"
     CDS             2662..3144
                     /gene="lysM"
                     /locus_tag="PKN51_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNT85754.1"
                     /translation="MGLFSFIKEAGEKLFGHKEVEQAVATGAPNLAELNQKAADAIKT
                     YVETQNLGLSGLTVGFDGASGKVTLGGNAPSQEAAEKAGLAAGNVASVSDVDNNLQYP
                     AGTASQYHDVVRGDTLSAIAKKYYGDANKYPVIFEANKPMLSHPDKIYPGQKLRIPPL
                     "
     gene            3255..4703
                     /locus_tag="PKN51_11450"
     CDS             3255..4703
                     /locus_tag="PKN51_11450"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNT85755.1"
                     /translation="MHAAAMPTDPTELDASALSAAIHARQLSCRELMAACLARIQRLN
                     PRHNALVSLRPDDELLAEADACDAELARGHSRGWLHGIPQAIKDLAHAAGLPTTLGSP
                     LMKDFIAREDGLMTARMKAAGCIVIGKSNTPEFGLGSHTFNPVFGTTLNAYDPSKSAG
                     GSSGGAAVALALRLLPVADGSDFMGSLRNPAAWNNLFGLRPSQGRVPMAPAQDVWISQ
                     LGTEGPMARSVRDLARLLATQAGWSPDAPLSIAAGADLASASMPLNLKGLRIGWLADV
                     DAYLPLEAGVLDTCTQALARFERAGSVVEPARLGMSPERVWDAWLVWRRVLVASRIAP
                     FLLKTGNRAHIKPEALWEHDQAQGCSAAEFMAASVTRSAFYQRMRALLARHDVLALPS
                     AQVWPFDARERWPQQIAGRTMDTYHRWMEVVIYASFAGLPSISVPAGFGSKGLPMGLQ
                     LIGQPQGDASLLRVAAAYEALIDDWLATMPPV"
     gene            4865..5494
                     /locus_tag="PKN51_11455"
     CDS             4865..5494
                     /locus_tag="PKN51_11455"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HNT85756.1"
                     /translation="MKPSRIASLVCATLVTAAAQAANVNLPGGTLVIPGTSPAGASFT
                     YTGTLTQADTIAFSQTGNPCLQSGNTYCVNGAGVIVVAGTSPVGASTGFGSWTYGALL
                     MQISGVGTVQVWPTNAANGLGSGSPPTGLTLPTTTLAALGFPAFSQANPTITFTVADT
                     NFGDNSESFTLTQAAAPAAVAPVPTLAEWSLGALALLVGLGGAARARRR"
     gene            complement(5522..5959)
                     /locus_tag="PKN51_11460"
     CDS             complement(5522..5959)
                     /locus_tag="PKN51_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNT85757.1"
                     /translation="MAGAPLAPDEHGVRAAVGYAGDMSPATAWAWVQAGRAALVDVRT
                     DAEREWVGFVPGALAVAWKQWPGMALNPDFDAQMRAAGQGGRPLLLICRSGVRSIAAA
                     RRATELGLTAYNVTEGFEGDPDQHAQRGHLGGWRHAGLPWRQN"
     gene            complement(6143..6664)
                     /locus_tag="PKN51_11465"
                     /pseudo
     CDS             complement(6143..6664)
                     /locus_tag="PKN51_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(6690..7109)
                     /locus_tag="PKN51_11470"
     CDS             complement(6690..7109)
                     /locus_tag="PKN51_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNT85758.1"
                     /translation="MPESDALQSHDEARMRLRRRVATLLWRALALLSLLLGGVGAMLP
                     VLPTVPFLLLAAWAASKGWPALEIWLLNHRRLGPPLVQWRERGAVSRPSKWLSSIMMG
                     GSAIGMQFLPQIPLALRLGAPAVMLAVAIWLWRRPEA"
     gene            7278..8153
                     /locus_tag="PKN51_11475"
     CDS             7278..8153
                     /locus_tag="PKN51_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNT85759.1"
                     /translation="MTRPAFLLLTLSTVMLGLASAASAGPLALWRALATLLLACLAHA
                     GGNVLNDYHDARNGADAANRNALSPFTGGARLIQDGQVTERDTARWALWLLLATSAGG
                     VVLASVSGAGLWLIGAAGLVLAWAYSAPPLALMARGLGEAAVAACWWLVVLGADYVQR
                     GALAALPALLGGGLALLVANLLLINGFPDAPADARVGKRTLVVRLGPAAAARLYLGLA
                     LLAHAVVPLAVLAGWAPTGALAGLLSLPLSMAAGGLLWRHRRQAARLRPAIGLTIAAA
                     NLHALALAAGLVLGR"
BASE COUNT         1257 a         2840 c         2887 g         1267 t
ORIGIN      
        1 gatgaccata gcgaggtggt cccactcctt cccatcccga acaggacagt gaaacacctt
       61 agcgccgatg atagtgcgga ttcccgtgtg aaagtaggac atcgtcaggc tattacagca
      121 agaaacgccc agtcagcaat gactgggcgt tttcttttgg gcggccgcat cgccccttgc
      181 ggagcggagt gaaagacgct tctcggtagc acagcctaga atgccctcga tgaacacacg
      241 caatatcaag catcaccatc tggccacggc gcgcgcattg ttgctggacc cgtatggact
      301 gtccgagcgg gagctggatg ccacgctggc gaccatcgcc cgtcaccgtg tcgacgacgc
      361 cgacctgttc tttcagtaca cgcgcaccga gggttggagc ctggaagagg ggatcgtcaa
      421 gacagggagt ttttcgatcg accagggcgt cggggtgcgg gcggtaacgg gtgagcgcac
      481 ggcgttcgcg tattcggacg atctttctcc cggttcgctg caggatgcgg catgcacggt
      541 gcgtgccatt tcggctgctg cgggggtggg gcgcgtcgat cggcgtcgcc gtcgtgcgtc
      601 cgggcgctat cgccccctgt atgcgccgat cgatccgatc gccacgttgg acagcggggc
      661 caaggtggcc ttgcttgaaa tggtggagca acgcgcgcgt gccagggatc cgcgcgtggt
      721 gcaggtgatg gcagggctgg cgagcgaata cgacgtggtg ctggtcgcgc gccttgatgg
      781 tacgctggcg gccgatgtgc gtccgctggt gcggctgtcg gtcacggtgg tggccgagca
      841 ggggggacgg cgcgagatcg gctcgtccgg cggtggcggg cgctttgggc tgacgcactt
      901 cgatgccagc cgggtggcgc agtacgtgga cgaggcggtc gccgccgcct tgaccaacct
      961 cgaatcaagg ccggcgccgg ccggcgaaat gaccgtggtc ctgggcccgg gttggccggg
     1021 cgtgttgttg cacgaggcgg tcggccacgg gctggagggt gacttcaacc gcaagggctc
     1081 cagcgctttc agcggccgca tcggccagcg cgtcgccgcc aaaggcgtga cggtgctcga
     1141 tgacggcacc ctggccgatc gccgcggcag cctgaacgtg gacgacgagg gccatgtgac
     1201 gcagcgcaat gtcctgatcg aagatgggat cctccggtcc tatttgcagg acaccctgaa
     1261 tgcccgcctg atgaaggtca aacccaccgg caacgggcgg cgtgagagct acgcccacat
     1321 cccgatgccc cgcatgacca acacctacat gctgggcggc gaccgcgacc cgcgcgaaat
     1381 cgtcgccagc atccgcaagg gcctgtacgc caccaacttc gggggcggcc aggtcgacat
     1441 caccagcggc aagttcgtgt tttcggccag cgaggcctac tgggtcgaga acggccggat
     1501 tcaatatccg gtcaagggcg ccacgctcat cggcagcggg cccgaatcgc tcaagaagat
     1561 cagcatgatc ggcaacgaca tgcgcctgga ctcgggtgtc ggtacctgtg gcaaggaagg
     1621 ccagagcgtg ccggtcgggg tcggtcagcc cacgctgcgc atcgacggtc tgacggtggg
     1681 cggaaccggt tgagtggccc gacggtaggc gtcgctgcgc gcgtcaggtg cgccatgcga
     1741 ggcggcgggc agggcccgca gcgctcgcta caacccgccg ttcagggcct cgcgtgcctc
     1801 ggcgatgacc agcagaccgt cgaagatcat gctctcgatc aactcatagg gccggctcat
     1861 ggccggcagc atcttccagc cggcgccgcc cgtcatcagg cacagcggct cggccgcaca
     1921 gcgtttgaac aggtgctgga acatgcgctc gcaggcgccg gcgatggcga aggtgccgcc
     1981 gctggtcagc gcatcgctgg tgttggtggg gaactccacc acgtcgccgg tgggcacatg
     2041 caggccggcc gtgccggact cgagcgcacg cagcatgatg ccgtgcccgg gaatgatcaa
     2101 cccgcccaga aagcgcccct gcgcgtcgat ggcctccacc gtcacggcgg tgccgaccat
     2161 caccagcagc accgggcggg ccggtccgcg ctgcagcatg tggtggcgcg cgccgaccat
     2221 ggccacccag cggtcggcgc ccaggcgcga ggggaagtcg tagccgttga ccaaccctgc
     2281 ttccgactcc gacgccacca cccagcgtgg ctccacgccc caatgctcca tctgctcctg
     2341 cacgcggtgc ttgacggcct ggccggcgac gatgcagccc agcatgcggc tgggttcggg
     2401 caggtcggcc cagggtcctt cggccagccg atcgagctgc tccagaaact ccacgccctg
     2461 cgccagcacc cgtgcaccga ccgccgggcg ctcatacagc gtccatttca gccgggtgtt
     2521 gccgacgtca atcgccaaaa aagtcatggg ttcgcaatat aagcgcattc gttgacgcct
     2581 ggataccgaa gtcacggcgc cgcgcgatcg tcctgcgtta gagtgaaagg ttcctgtcat
     2641 caacaaccaa ggagaacctg tatgggactg ttcagcttca tcaaggaagc cggcgaaaag
     2701 ctgtttggcc acaaggaggt ggagcaggct gtggccacgg gcgcacccaa cctggccgag
     2761 ctgaaccaga aggctgccga cgccatcaag acctacgtcg agacgcagaa cctgggcctg
     2821 tcgggtctga cggtcggctt tgatggcgcc agcggcaagg tcacgctggg tggcaatgcg
     2881 cccagccagg aagcggccga aaaggccggc ctggccgctg gcaacgtggc cagcgtcagc
     2941 gatgtcgaca acaacctgca gtacccggcc ggcaccgctt cgcaatacca cgacgtggtg
     3001 cgcggtgaca cgctgagcgc catcgccaag aaatactatg gcgatgccaa caagtacccg
     3061 gtgatcttcg aagccaacaa gccgatgctg agccacccgg acaagatcta cccgggccag
     3121 aagctgcgca tcccgccgct ctgaaatcaa tagcttatgg cgcttgccct gcatgcgccg
     3181 gagcctgatt tcgaccatga aaaggcatct tcggatgcct tttttcatgg ctgctgctgg
     3241 cgtagagtgg cgccatgcac gccgccgcca tgcccaccga cccgaccgag ctggacgcca
     3301 gcgcgctttc ggccgccatc catgcgcgcc agctgtcctg ccgcgagctg atggcggctt
     3361 gtctggcgcg catccagcgc ctcaacccgc gccacaacgc gctggtgtcg ctgcgccccg
     3421 acgacgaact gctggccgag gccgacgcct gtgacgccga actggcgcgc ggccactcgc
     3481 gcggctggct gcacggcatc ccgcaggcga tcaaggacct ggcccacgcc gccggcctgc
     3541 ccaccacgct gggttcgccg ctgatgaagg acttcattgc ccgggaagac ggcctgatga
     3601 ccgcacgcat gaaggcggcc ggctgcatcg tcatcggcaa aagcaacacg cccgagttcg
     3661 gcctcggctc gcacaccttc aacccggtgt tcggcaccac gctgaacgcc tacgacccga
     3721 gcaagtcggc cggcggctcc agcggcggcg cggcggtggc gctggcgctg cgcctgctgc
     3781 cggtggccga cggctccgac ttcatgggca gcctgcgcaa cccggcggcc tggaacaacc
     3841 tgttcggcct gcgcccctcg cagggccgcg tgcccatggc gcccgcgcag gacgtctgga
     3901 tcagccagct gggcaccgaa gggccgatgg cgcgcagcgt gcgcgatctg gcgcggctgt
     3961 tggccacgca ggccggctgg tctccagatg ctcctctttc gatagctgcc ggcgcagatc
     4021 tggcaagcgc cagcatgccg ttgaacttga aaggcctgcg catcggctgg cttgccgacg
     4081 tggacgcata tctgccgctg gaggccggtg tgctggacac ctgcacgcag gcgctggcgc
     4141 gcttcgagcg cgccggcagc gtggtcgagc cggcgcggct gggcatgtcg cccgagcgtg
     4201 tgtgggacgc ctggctggtc tggcggcgcg tgctggtggc ctcgcgcatc gcgccgttct
     4261 tgctgaaaac cggcaaccga gcccacatca agcccgaggc gctgtgggag cacgaccagg
     4321 cgcaaggctg cagcgccgcc gagttcatgg ccgccagcgt cacccgcagc gccttttatc
     4381 agcgcatgcg cgcactgctg gcgcgccacg acgtgctggc cctgcccagc gcccaggtct
     4441 ggccgttcga cgcccgcgaa cgctggccgc agcagatcgc cggccgcacc atggacacct
     4501 accaccgctg gatggaagtg gtgatctacg ccagcttcgc cggcctgccc tccatcagcg
     4561 tgcccgccgg cttcggctcc aaaggcctgc ccatgggcct gcagctgatc ggtcagcccc
     4621 agggtgacgc ctcgctgctg cgcgtggctg cggcgtatga agcgctgatc gacgactggc
     4681 tggcgacgat gcctccggtc tgacgggcat ggtgcagccc gtggcgacac cgtaaataaa
     4741 agttacattc attggcgttc gtcggtggcg ccgataccat gagcgcgcgg gcattgcagt
     4801 cgatggggtg tctgctttcc aaacaagatg ccggccaatg gtcggtttta ccggagacca
     4861 tcccatgaag ccaagccgga tcgcatcgct ggtgtgcgcc accctcgtca cggcggctgc
     4921 ccaggccgcc aacgtcaacc ttccgggggg tacgctggtg atcccgggca ccagccccgc
     4981 cggcgcgtca ttcacctaca ccggcacgct gacgcaggcc gacacgattg ccttcagcca
     5041 gaccggcaac ccctgcctgc agtccggcaa cacctattgc gtcaatggcg ccggcgtgat
     5101 cgtggtggcc ggtacttcgc ctgtgggggc gtcgaccggt tttggcagct ggacctacgg
     5161 ggcgttgctg atgcagattt cgggtgtcgg caccgtacag gtgtggccga ccaatgccgc
     5221 caacgggctc ggctccggct cgccgcccac cgggctgacg ctgccgacca cgacgctggc
     5281 ggcgctcggg tttccggcgt tttcccaagc caacccgacc atcaccttca ccgttgccga
     5341 caccaacttc ggcgacaaca gcgaaagctt caccctcact caggcggccg ccccggcggc
     5401 ggtggcaccg gtgcccacat tggccgaatg gagcctgggc gcgctggcgc tgctggtcgg
     5461 gctgggcggc gcagcccgtg cccggcggcg ctgagccgct ggcgcccggc cggttgcacc
     5521 gtcagttctg ccgccacggc aaccccgcat gccgccagcc gcccaggtgg ccgcgctggg
     5581 cgtgctggtc ggggtcgccc tcaaaacctt cggtcacgtt gtaggccgtc aggcccagct
     5641 cggtggcgcg gcgcgcggcg gcgatggagc gcacgccgct gcggcagatc agcagcagcg
     5701 gccggccacc ctgcccggcg gcgcgcatct gcgcatcaaa gtcagggttg agcgccatgc
     5761 cgggccactg cttccaggcc acggccagcg caccgggcac aaagccgacc cactcgcgtt
     5821 cggcgtcggt gcgcacgtcg accagtgcgg cgcggccggc ctgcacccag gcccaggcgg
     5881 tggcgggcga catatcgccg gcatagccca cggcggcgcg cacgccgtgc tcgtccggcg
     5941 ccagcggcgc acccgccatc tggtgcaggc ccgagcgccg gttggcgggc agcgcctcgt
     6001 cgatgcggcg cgggtgcggc aggttcaatg ccagcatcag ggccacgaac tcggcccgcg
     6061 tgcggccggc caggcgcgcg ttgtgagcgc gctcgtgccc cacgctggaa tgcgtgcggc
     6121 cttcgtagtc gtgccccggc cagacgcggg tgtcgtcggc cagcgtgaac agcttttgcg
     6181 tgatgctgtc gtacagctgg ccggcgtcgc ccgactgaaa gtcggtgcgc ccgcagccgt
     6241 cgatcagcag cgtgtcgccg gtgaacagct gcgccggtgc gcccgcctgg cgcggctgcc
     6301 acagatagca gacgctgccc gccgtgtggc cgggcgtgtg cagcacgcgc agctgctcgc
     6361 cgccgaagtc cagcgtgtcg ccgtcgcgca gctgcacggc ggcggtgccc acctcgcagg
     6421 cggccggagc gccgctgtgc gcgccggtca gttccaccag gcgcgcggcg ctggtgatgt
     6481 ggtcggcatg ggcgtgggtt tccagcgtcc acttcagcgt caggccctcg cgctccagca
     6541 gcgccaggtc gcgctcgagc tggccgtcga ccgggtcgat gatcaccgcc tggcgcgtgg
     6601 cggggtcggc cagcaggtag gtgtaggtgc agctggtcgg gtcaaagagc tggatcgggt
     6661 gcatggccgc cagtgtaggc gggcggcgct caggcctcgg gccgtcgcca cagccagatg
     6721 gccaccgcca gcatcaccgc cggcgcgccg aggcgcagcg ccagcggaat ctgcggcaga
     6781 aactgcatgc cgatggcgct gccacccatc atgatcgaac tcagccactt ggatgggcgc
     6841 gacaccgccc cgcgttcgcg ccactgcacc agcggcgggc cgagccggcg gtggttgagc
     6901 agccagatct ccagcgccgg ccagcccttg ctggcggccc aggcggccag cagcagaaac
     6961 ggcaccgtcg gcaacaccgg cagcatggcc ccgacgccac ccagcagcag gctcagcagc
     7021 gccagcgcgc gccacagcag cgtggcgacg cgccggcgca gtcgcatgcg cgcctcgtcg
     7081 tggctttgca gggcgtcaga ttcaggcatc aaatcggctt gcagcgcttg atgttcaagc
     7141 gccagcagct attaaaatag tagcttatga acgattcatc gcatcttacc cggggcgcgc
     7201 cccaggcgcc atcgggtggg ggttcgcagg cgccggccgc tgcgcgctgg cgccaccagg
     7261 cgtggctgcg ctggcgcatg acgcgaccgg cctttctgct gctgacgctg tcgaccgtca
     7321 tgctcgggct ggccagcgcc gccagcgccg gaccgctggc actctggcgc gcgctggcga
     7381 cgctgctgct ggcctgtctg gcgcacgccg ggggcaacgt gctgaacgac tatcacgacg
     7441 cccgcaacgg tgccgacgcc gccaaccgga acgcgctgtc gcccttcacc ggcggtgcgc
     7501 gcctgattca ggacggccag gtgaccgagc gcgacaccgc gcgctgggca ctctggctgc
     7561 tgctggccac gagcgccggc ggggtggtgc tggccagcgt cagcggcgct gggctgtggc
     7621 tgatcggcgc cgccgggctg gtgctggcct gggcctattc ggccccgccc ctggcgctga
     7681 tggcgcgcgg gctgggcgaa gccgcggtgg ccgcctgctg gtggctggtg gtgctggggg
     7741 cggactacgt gcagcgcggc gccctggccg ccctgccggc gctgctgggt ggcgggctgg
     7801 cgctgctggt ggccaacctg ctgctgatca acgggtttcc cgacgcgccc gccgatgcgc
     7861 gggtcggcaa gcgcaccctg gtggtgcgcc tggggccggc cgcggcggcg cgcctgtacc
     7921 tggggctggc gctgctggcg cacgcggtgg tgccgctggc ggtgctggcc gggtgggccc
     7981 cgacgggcgc gctggccggc ctgctgtcgc tgccgctgtc aatggccgcc ggcgggctcc
     8041 tgtggcgcca tcgccggcag gcggcgcggc tgcggccggc catcggcctg accattgccg
     8101 ccgccaacct gcacgccctg gcgctggcgg cggggctggt gctggggcgc tgaggcggac
     8161 gcggtttcag cctcctagaa acggccgttg gacgcgcccg cccgtgccgt gatcggcgtg
     8221 ccaggcaaga cgcgacgacg ccgcgggctc c
//
LOCUS       DAOQTZ010000112         8147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_9757_length_8147_cov_15.519155, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000112 DAOQTZ010000000
VERSION     DAOQTZ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9757_length_8147_cov_15.519155"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1236)
                     /locus_tag="PKN51_11480"
     CDS             complement(211..1236)
                     /locus_tag="PKN51_11480"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNT85760.1"
                     /translation="MKIIVIGAGAWGTALAVSAARNPAGHQVVLWARDGAQAQAMAEQ
                     RENARYLPGIPLPRELQVRGGEVAGLLQAVPQAELLIVATPVAGLRDTLRLLRGVPVP
                     VGWLCKGFEPAQDPGAAHPFGLMPHEIQRQVAPELRAGALSGPSFAQEVARGQPTALV
                     AASRHADVRDALVAGFHGPTLRVYANDDLIGVEVGGAVKNVLAIATGLADGLNLGLNA
                     RAALITRGLTEMARLGLALGARADTFMGLSGLGDLVLTATGDLSRNRRVGLLLAEGKT
                     LEEAVRSLGHVAEGVYSARTVWQRAQHLGVEMPITESVVALLDGRLKPVEAVAALMGR
                     DPKGELL"
     gene            complement(1282..1734)
                     /gene="secB"
                     /locus_tag="PKN51_11485"
     CDS             complement(1282..1734)
                     /gene="secB"
                     /locus_tag="PKN51_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNT85761.1"
                     /translation="MADQDPVFNIQRVYLKEASLEQPNSPAILLEQQQPAVEISLGVN
                     AENVADGIFEITVTATVQTKIEDRTVFLCECKQAGIFEVRNVPDEQMGAVIGIACPQI
                     VYPYLRGNVADLIQRGGFPPVHLAEINFQQMYEQQQAAQADGASALPQ"
     gene            complement(1855..2112)
                     /gene="grxC"
                     /locus_tag="PKN51_11490"
     CDS             complement(1855..2112)
                     /gene="grxC"
                     /locus_tag="PKN51_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNT85762.1"
                     /translation="MPTVRMYATATCPYCLRAKQLLAHKGVTAIEEIRVDLEPAQRRA
                     MMALTGRHTVPQIFIGDTHVGGCDDLMALEARGGLDALLAG"
     gene            complement(2140..2544)
                     /locus_tag="PKN51_11495"
     CDS             complement(2140..2544)
                     /locus_tag="PKN51_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNT85763.1"
                     /translation="MDFLLANWMLILVALTSGTMLLLPLLQGGGAGLTPTAAVQLINR
                     DKAVVVDVCGTDEFAAGHVAGARSIPLHELEARLPGSVKNKATPLVLVCASGMRSGRA
                     VAIARKLGYENAQSLAGGLKAWREANLPVERG"
     gene            2721..3467
                     /gene="gpmA"
                     /locus_tag="PKN51_11500"
     CDS             2721..3467
                     /gene="gpmA"
                     /locus_tag="PKN51_11500"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNT85764.1"
                     /translation="MYKLVLIRHGESTWNLENRFTGWTDVDLTPLGIEQACAAGRLLK
                     AEGYDFDVAYTSVLTRAIRTLHLALDEMNRLWLPTVKHWRLNERHYGALQGLNKAETA
                     RQYGDQQVLVWRRSYDTPPPALTPDDPRCERSDPRYARLNPGEVPLTECLKDTVARVL
                     PFWNESMAPAIRSGKRLLVSAHGNSIRALVKYLDGISDDDIVGLNIPNGIPLVYELDA
                     DLRPLRPHYYLGDADAIARAAAAVASQGRA"
     gene            3605..5038
                     /locus_tag="PKN51_11505"
     CDS             3605..5038
                     /locus_tag="PKN51_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNT85765.1"
                     /translation="MSQKTRIAGWIAVGALAGALTTVSLQTVARGTLAPLPLEELQQL
                     AAVFGMIKSDYVEPVDEKKLITDAISGMVSSLDPHSQYFDKKSFKEFREGTTGRFVGV
                     GIEITQEDGLVKVVSPIEGSPADRAGLKSGDLITKVDDTAVKGLSLNEAVKRMRGEPG
                     TKVLLTILRKDENRTFPVTITREEIRTQSVKAKVVEPGYAWLRLSQFQERTVDDFVTK
                     LEDIYRREPNLKGLVLDLRNDPGGLLDAAVAVSAAFLPEGVTVVSTNGQIEESKSIYK
                     ARPEDYLRRRGVDPLRRLPAALKNVPLVVLVNEGSASASEIVAGALQDHKRAIVMGSQ
                     TFGKGSVQTVRPLGPDTALKITTALYYTPSGKTIQAKGIVPDILIDETAEGSPFAVLR
                     MREADLDKHLNGGEAEKKDPALEKEREAAMKRLEEESRKPAAQRRPPEFGSAQDFPLQ
                     QALAKLKGQPVQVSKTLALRKEEPKTD"
     gene            5068..5862
                     /locus_tag="PKN51_11510"
     CDS             5068..5862
                     /locus_tag="PKN51_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNT85766.1"
                     /translation="MDDRQLLRYSRHLLLDDIGVEGQQRLLDAHALIVGAGGLGSPVA
                     LYLASAGVGQLTLVDHDTVDLTNLQRQIMHSQGRIGLPKVASAAEAIQAINPEVRVHT
                     VARRGDLPLFCALAAEADVVLDCSDNFATRQALNQACVASGRPLVSGAAIGFDGQVSV
                     YDLRRPDAPCYACLFPPSQTVEEVRCATMGVFAPLVGIIGTLQAAEALKLLAGIGRSL
                     SGRLLMLDARQMEWTEVRVPRQPACPVCRGRDSVPAASRQADPASS"
     gene            5924..6268
                     /locus_tag="PKN51_11515"
     CDS             5924..6268
                     /locus_tag="PKN51_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85767.1"
                     /translation="MNPQDTPDKSADATERTIETARRYANEALDRADAGVRNLREDVQ
                     PAIDALSARVQELAERGKALASQTSAQTREKLNEYSEKTSTYVAQQPLKSMAIAAAAG
                     AALALLLGRRRD"
     gene            complement(6487..6972)
                     /locus_tag="PKN51_11520"
     CDS             complement(6487..6972)
                     /locus_tag="PKN51_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT85768.1"
                     /translation="MPVARLHQPLTLDDMVLYVLWNIQAGAGRAVVRLCENEFGITRR
                     QWRLLAHLAQHEGLQPSELALHVGLDRARTSRALTGLVHKQLVRRLPRPGNRREATLQ
                     LTAEGAALHARLLPRVADINRLLLSVLSEAEIDQLADALARLQAQARRMGGQANSAEA
                     A"
     gene            7117..8028
                     /locus_tag="PKN51_11525"
     CDS             7117..8028
                     /locus_tag="PKN51_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HNT85769.1"
                     /translation="MSAPAHAQNDAPLRLIVPYAPGGSSDRAARIVADKLTARLGAPV
                     VVENKTGAGGRLAMQQSRGVPASQNVLVLANPATMVVAPLVFKDNGYDPDRDFRPVSQ
                     VSDYEFGVAVSTAVPVKEIGHLLAWLRANPEQANFGVPATGSLPHFFALMTGERAQIK
                     AQVVGYRGSGPLLTDLIGGQIPVAFDTFDTLLPQHEAGKLRILGSSGARRSPFAAGVP
                     TFRESGLDLVASGWNAFFAPASMPAARVASLGRAIREVMQEQDTRRKFHDARMVPVAS
                     SPEQTAAMLKAYRAQWAPVVQKSGYQP"
BASE COUNT         1304 a         2960 c         2733 g         1150 t
ORIGIN      
        1 ggccatcgca gccgcccgac cccgcatcga tgcggtctgc ccgaggttga caagcggtca
       61 accgccgttt ccaggctgag cctgcttgct cccaattcag gagctgccgg cgctgactgc
      121 aagcgcgccg gggtcagatc cgaccaggaa cccggcaccc cgcccgagac cgccccaggc
      181 atcgaagcgc agcgaggtga tgcgccacgc tcagagcagc tcgcccttgg gatcgcgccc
      241 catcagcgcc gccaccgctt ccaccggctt gaggcggccg tccagcagcg ccaccacgct
      301 ttcggtgatc ggcatctcca cccccaggtg ctgcgcgcgc tgccacacgg tgcgcgcgct
      361 gtacacgccc tcggccacgt ggccgagcga gcgcaccgcc tcctccagcg tcttgccctc
      421 ggccagcagc aggccgacgc ggcggttgcg gctcagatcg ccggtggccg tcagcaccag
      481 atcgcccaga cccgacagac ccatgaaggt gtcggcgcgc gcgcccagcg ccagccccag
      541 gcgcgccatt tcggtcagcc cgcgggtgat gagggcggcg cgtgcgttca gccccaggtt
      601 caggccgtcg gccaggccgg tggcgatggc cagcacgttc ttgaccgcgc cgcccacttc
      661 gacgccgatc aggtcgtcgt tggcgtacac gcgcagcgtg gggccgtgaa aacccgccac
      721 cagggcgtcg cgcacgtcgg catggcggct ggccgccacc agcgccgtgg gctggccgcg
      781 cgccacttcc tgggcaaagc tgggtccgct gagcgcgccg gcgcgcagct cgggcgccac
      841 ctggcgctgg atctcgtgcg gcatcaggcc gaaggggtgc gccgcgccgg ggtcctgcgc
      901 cggctcgaag cccttgcaca gccagccgac cggcaccggc acgccgcgca gcaggcgcag
      961 ggtgtcgcgc agcccggcca ccggggtggc gacgatcagc agctcggcct gcggcacggc
     1021 ctgcagcagc ccggccactt cgccgccgcg cacctgcagc tcgcgcggca gcggaatgcc
     1081 gggcaggtag cgcgcgttct cgcgctgctc ggccatggcc tgcgcctgcg cgccgtcgcg
     1141 cgcccaaagc accacctggt gaccggccgg gttgcgcgcc gcactgacgg ccagcgccgt
     1201 gccccaggca cccgcaccga taactatgat tttcatagct gctggcgctt acccatcaag
     1261 cgcgggaggc agatttgatg cttactgcgg cagggccgag gcgccgtcgg cctgggccgc
     1321 ctgctgctgc tcgtacatct gctggaagtt gatctcggcc aggtgcacgg gcgggaagcc
     1381 gccgcgctgg atcaggtcgg ccacgttgcc gcgcaggtag gggtagacga tctgcgggca
     1441 ggcgatgccg atcaccgcgc ccatctgctc gtcgggcacg ttgcgcacct cgaagatgcc
     1501 ggcctgcttg cattcgcaca ggaacaccgt ccggtcttcg atcttggtct gcacggtggc
     1561 ggtgacggtg atttcgaaga tgccgtcggc cacgttctcg gcgttgacgc ccaggctgat
     1621 ttcgaccgcc ggctgctgtt gctccagcag gatggcgggc gaattgggct gctccagcga
     1681 ggcctccttc aggtagacac gctggatgtt gaacacgggg tcctgatcgg ccatgggatt
     1741 tctctctggg atgggaaggg ggtgaaagcc ggcgtggcgc ggccgccgag gcggcgcgcc
     1801 cgtcgtgaaa agccggccat tatctcagag gggctcaccc cggccggcag gggctcagcc
     1861 ggccagcagc gcgtccagcc cgccgcgcgc ctccagcgcc atcaggtcgt cgcagccgcc
     1921 cacgtgggtg tcgccaatga agatctgcgg cacggtgtgg cgaccggtca gcgccatcat
     1981 cgcgcggcgc tgcgccggct ccagatcgac gcggatttcc tcgatggcgg tcacgccctt
     2041 gtgcgccagc agctgcttgg cgcgcaggca gtaggggcag gtggcggtgg cgtacatgcg
     2101 cacggtgggc atggcggggc tcggatcgac ggccatggct cagccgcgct cgaccggcag
     2161 gttggcctcg cgccaggcct tcagcccgcc ggccagcgac tgcgcgttct cgtagcccag
     2221 cttcctggcg atcgccaccg cccggccgga gcgcatgccc gaggcgcaca ccagcaccag
     2281 cggcgtggcc ttgttcttca cgctgccggg cagccgcgcc tccagctcgt gcagcgggat
     2341 gctgcgcgcg cccgccacat ggccggcggc gaattcgtcg gtgccgcaga catcgaccac
     2401 caccgccttg tcgcggttga tcagctgcac cgcagcggtc ggcgtcaggc cggcgccgcc
     2461 gccctgcagc agcggcagca gcagcatggt gccggaggtc agcgccacca ggatcagcat
     2521 ccagttcgcc agcagaaaat ccacgtcgtc ggtccttctc ttgagggtgt tcgggcgacc
     2581 atggggccgc cccggggcgg cccgcggccg cctgcacgga cggggattct aaaatggcgg
     2641 gttatgcccc gtgcctgcgc ggcctttgcc ggcgccgcca cggcccgccc cacctgcctg
     2701 accctgccgt accgctgccc atgtacaagc tcgtgctgat tcgccatggc gaatcgacct
     2761 ggaacctcga aaaccgcttc accggctgga ccgacgtcga cctgacgccg ctgggcatcg
     2821 agcaggcctg cgccgccggc cggctgctga aggccgaggg ctacgacttc gacgtcgcct
     2881 acaccagcgt gctgacgcgc gccatccgca ccctgcacct ggcgctggac gaaatgaacc
     2941 gcctgtggct gccgaccgtc aagcactggc gcctgaacga gcgccactac ggcgccctgc
     3001 agggcctgaa caaggccgaa accgcgcgcc agtacggcga ccagcaggtg ctggtctggc
     3061 gccgcagcta cgacaccccg ccgccggcgc tgacgcccga cgacccgcgc tgcgagcgca
     3121 gcgacccacg ctacgcgcgc ctgaacccgg gcgaagtgcc gctgaccgag tgcctgaagg
     3181 acacggtggc gcgcgtgctg ccgttctgga acgaatccat ggcgccggcc atccgctccg
     3241 gcaagcgcct gctggtgtcg gcgcacggca actcgatccg cgctctggtc aagtacctgg
     3301 acggcatctc cgacgacgac atcgtgggcc tgaacatccc caacggcatc ccgctggtgt
     3361 acgagctgga cgccgatctg cgcccgctgc gcccgcatta ctacctgggc gatgccgacg
     3421 ccatcgcgcg cgccgccgcc gcggtggcca gccagggccg cgcctgaggc cgcatccagg
     3481 cgcccggcgc ggtcagcccc cgcgtcgccc gcggccgcgt tgcggaactt cacacgcccg
     3541 gccggacccc aacgcaggcc gttaggtata tattgagcct ctgcgacgca ggaaccccca
     3601 gaccatgagt cagaaaacca ggattgccgg atggatcgcc gtgggcgcgc tggccggcgc
     3661 gttgaccacc gtgtcgctgc agaccgtggc gcgcggcacg ctggccccgc tgccgctgga
     3721 agagctgcag caactggcgg cggtgttcgg catgatcaag tccgactacg tggagccggt
     3781 ggacgagaaa aagctcatca ccgacgccat ctcgggcatg gtgtccagcc tggatccgca
     3841 ctcgcagtat ttcgacaaga agagcttcaa ggaattccgc gaaggcacca ccggccgctt
     3901 cgtcggcgtc ggcatcgaaa tcacccagga agacgggctg gtcaaggtgg tctcgcccat
     3961 cgaaggctcg ccggccgacc gcgccggcct gaagtcgggc gacctgatca ccaaggtgga
     4021 cgacaccgcc gtcaagggcc tgagcctgaa cgaggcggtc aagcgcatgc gcggcgaacc
     4081 gggcaccaag gtgctgctga ccatcctgcg caaggacgaa aaccgcacct tcccggtcac
     4141 catcacgcgc gaagagatcc gcacccagtc ggtcaaggcc aaggtggtcg agcccggcta
     4201 cgcctggctg cgcctgtcgc agttccagga gcgcacggtg gacgacttcg tcaccaagct
     4261 ggaagacatc taccggcgcg agcccaacct gaagggcctg gtgctcgatc tgcgcaacga
     4321 cccgggcggc ctgctggacg cggcggtcgc ggtatcggcc gccttcctgc ccgagggtgt
     4381 gacggtggtg tcgaccaacg gccaaatcga ggaaagcaag tccatctaca aggcccgccc
     4441 cgaagactac ctgcgccgcc gtggcgtcga tccgctgcgg cgcctgccgg cggcgctcaa
     4501 gaacgtgccg ctggtggttc tggtcaacga gggttcggcc tcggccagcg aaatcgtggc
     4561 cggcgccctg caggaccaca agcgcgccat cgtcatgggc agccagacct tcggcaaggg
     4621 ctcggtgcag acggtgcgcc cgctcggccc cgacacggcg ctcaagatca ccaccgcgct
     4681 ctactacacg cccagcggca agaccatcca ggccaagggc atcgtgcccg acatcctgat
     4741 cgacgaaacc gccgaaggca gtccgttcgc cgtgctgcgc atgcgcgagg ccgatctcga
     4801 caagcacctg aacggcggcg aggccgagaa gaaggacccg gcgctcgaaa aagagcgcga
     4861 agccgccatg aagcgcctcg aagaagaatc gcgcaagccg gccgcccagc gccgcccgcc
     4921 cgaattcggc agcgcgcagg acttcccgct gcagcaggcg ctggccaagc tcaagggcca
     4981 gccggtccag gtcagcaaga cgctggcgct gcgcaaggaa gagcccaaga ccgattgagc
     5041 ccgcgccgca gcgcgggacg ggcgccgatg gacgaccggc aactgctgcg ctattcgcgc
     5101 cacctgctgc tcgacgacat cggcgtcgag ggtcagcagc ggctgctgga cgcgcacgcg
     5161 ctgatcgtcg gcgccggtgg cctgggctcg ccagtggcgc tgtacctggc cagcgcgggc
     5221 gtcgggcagc tgaccctggt cgatcacgac acggtcgatc tgaccaacct gcagcgccag
     5281 atcatgcaca gccaggggcg catcggcctg cccaaggtgg cctcggccgc cgaagccatc
     5341 caggccatca atcccgaggt gcgtgtgcac accgtggcac gccgcggcga cctgccgctg
     5401 ttttgcgccc tggccgccga agccgacgtg gtgctggact gcagcgacaa cttcgccacc
     5461 cgccaggcgc tcaaccaggc ctgcgtggca agcggccggc cgctggtgtc cggcgccgcc
     5521 atcggcttcg acggccaggt gtcggtgtac gacctgcgcc gccccgacgc gccctgctat
     5581 gcctgcctgt tcccgcccag ccagaccgtg gaagaggtgc gctgcgccac catgggcgtg
     5641 ttcgcgccgc tggtgggcat catcggtacc ctgcaggcgg ccgaggcgct caagctgctg
     5701 gccgggatcg ggcgctcgct gagcggccgg ctgctgatgc tggacgcgcg ccagatggaa
     5761 tggaccgagg tgcgcgtgcc gcgccagcct gcctgcccgg tgtgccgcgg ccgcgacagc
     5821 gtgccggccg ccagccgcca ggccgacccg gcttcgtcct gaccgccacc gcgccgggtg
     5881 caacaatcgc cctttgcccc tcgcaccctc acaggagatg cccatgaacc cgcaagacac
     5941 ccctgacaag tcggccgacg ccaccgagcg caccatcgaa accgcgcgcc gctacgccaa
     6001 cgaggccctg gaccgcgccg acgccggcgt gcgcaacctg cgcgaggacg tgcagccagc
     6061 catcgacgcc ctgtcggcgc gggtgcagga actggccgag cgcggcaagg cgctggcgtc
     6121 ccagacctcg gcgcaaacgc gcgagaagct gaacgagtac agcgaaaaga ccagcaccta
     6181 cgtggcccag cagccgctca aatccatggc catcgccgcc gccgccggcg ccgccctggc
     6241 cctgctgctg ggccgacggc gcgactgatc ctaggaggct gtcgggcttg gagcgccgaa
     6301 agcgagaatc ggccccagcg agaccagttt ttgccggatt tgcagcccaa tagtcgagct
     6361 attgggcaaa aagccggtga aaaatgggcc gctgcggtcg atttgcagcc ggcgatttcc
     6421 aagtccgaca gcctcctagt ccactgaaac actgaaatcg gatcggcggc gcgccgcccg
     6481 gcaagctcag gcggcttcgg cggagttggc ctggcctccc atccggcgcg cctgcgcctg
     6541 caaccgggcc agcgcatcgg ccagctggtc gatttccgcc tcgctgagca ccgacagcag
     6601 aagccggttg atgtcggcca cccggggcaa cagcctggcg tgcagggccg cgccttcggc
     6661 cgtcagttgc agcgtggctt cacgtcggtt gcccggccgg ggcaggcgcc gcaccagttg
     6721 cttgtgcacc agcccggtca aggcccgcga ggtgcgcgcg cgatccaggc ccacgtgcag
     6781 tgccagctcc gacggctgca gtccctcgtg ctgcgccaga tgcgccagca gtcgccactg
     6841 gcgccgcgtg atgccgaact cgttctcgca caaacgcacc accgcccgac cggcgcccgc
     6901 ctggatgttc cacagcacgt agagcaccat gtcgtcaagc gtcaggggct ggtgcaggcg
     6961 agccacgggc atacgggtta atcctgagaa tgattgattt tgtcaattga tggtcatcaa
     7021 actacagtcg ctcattttgt agtaggcacc ctcaggagac aagccttgaa ccgacgatcc
     7081 ctgctgctgg ccggcaccag tctggccgtg ggcgcgatga gcgccccggc ccacgctcaa
     7141 aatgacgccc ccctgcgtct gatcgttccc tacgcccccg gtggctccag cgaccgcgcc
     7201 gcccgcatcg tggccgacaa gctgaccgcc aggctgggcg caccggtggt ggtcgaaaac
     7261 aagaccggcg ccggcggccg cctggccatg cagcaaagcc gcggcgtgcc cgccagccag
     7321 aacgtgctgg tgctggccaa cccggcgacc atggtggtgg cgccgctggt gttcaaggat
     7381 aacggctacg acccggacag ggatttccgg ccggtgtcgc aggtcagcga ctacgaattc
     7441 ggcgttgccg tctccacggc cgtgccggtc aaggagatcg ggcacctgct ggcttggctg
     7501 cgcgccaacc ccgaacaggc caatttcggc gtgccggcca ccggcagcct gccgcatttc
     7561 ttcgccctca tgaccggcga gagggcgcag atcaaggccc aggtggtggg ttaccgcggc
     7621 tcggggccgc tgctgaccga cctgatcggc ggccagatcc cggtcgcctt cgacaccttc
     7681 gacaccctgc tgccgcagca cgaggcgggc aagctgcgca tcctgggttc ctccggcgcg
     7741 cggcgctcgc ccttcgctgc cggcgtgccg acattcaggg agtcggggct ggatctggtg
     7801 gccagcggct ggaacgcctt cttcgccccg gccagcatgc cggccgcgcg cgtggccagc
     7861 ctagggcggg ccatccgcga ggtgatgcag gagcaggaca cgcggcgcaa gttccacgac
     7921 gcgcgaatgg tgccggtggc cagcagcccc gagcagaccg ccgccatgct caaggcctac
     7981 cgcgcgcagt gggcgccggt ggtgcaaaag tcgggctacc agccctgacg ccgccgggcg
     8041 gcgccggccg cccatccccg cttccttccc tcttggctcg ccgcatcgcg gcgcatcacc
     8101 catgtctcga accctcaaac tgattcgcac caccctgggc ctgacct
//
LOCUS       DAOQTZ010000113         8005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_9972_length_8005_cov_12.985786, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000113 DAOQTZ010000000
VERSION     DAOQTZ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9972_length_8005_cov_12.985786"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKN51_11530"
     CDS             <1..363
                     /locus_tag="PKN51_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT85770.1"
                     /translation="ATRQIAALVKAIQTDTQDAVAAMERSTQGVVEGARLSDNAGTAL
                     SEIDRVSRQLAELIERISASASKEADQANEVAGNIQHIFAVTEQTGEGTRTTATQVRE
                     LARMADELRQSVARFKIA"
     gene            428..>3705
                     /locus_tag="PKN51_11535"
     CDS             428..>3705
                     /locus_tag="PKN51_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNT85771.1"
                     /translation="MHDIMVMSPAAEMRDSGASAVRDLGPLAWVLDETRRSIEAASKS
                     LKRFARETDAARGIDLGSVDASPLRMARQQLHQVAGALDMVDQSVAALMVRGMEGAVQ
                     QFVQRPDKCTEETAQALERAGFALMEYLEAQMGSHPRSAVGLFPQYRRVQELAGADRI
                     HPADLWSVPWSWADPVVPASAQPLSYDPEVRHRLDQRVLKILRHPAAHAADELAVISL
                     SLAAGQMQRQATVFWLLSAGFFEALGRGLIVKDVYAKRAASRILMQYTSLARGGQVVS
                     ERLAHDLLFFCSQARFDADANTPALQAVRKAWSLDAQQATSYEQAMFGLYDPAILAQA
                     RRRIESVKENWSALTGGDLARMKACVDQFSLVAESLDKLHEQGSVLGEVLNRVAQQTA
                     QSGRPPGPELAMETATAVLFLEASFADFHPGDAMFSTRLAQLAARLDAVRAGASAPPL
                     EPWMEELYRNVSDRQTMGTVVGELRTTLGEVEHLLDQFFRQPADRSTLVSVPGLMGQM
                     RGVLSVLGLDQAALAVGRMRDLVDELLAAQPDTKLADSAPSFEKLGDTLGALGFQIDM
                     LSYQPVLARKLFVYDADAGELRYRSGRAATQVTTEQNSVASATTDIDLLLDAGTELTV
                     PTSGPGSLVDESLPSETISGQLDEIATMAALAEQRALADAAQRAADAARRHDEAGLSG
                     ALDALQALTAASTPQPTSTTVEGGGADDDDLQAIFLEEAQQVMDTGCEVVQALLTAPG
                     DMELQTALRRAFHTLKGSSRMVGLTEFGEAAWAMEQLFNAWLPEQKPVTEDMRGLALE
                     ALQGLHAWTEAIAGRNDHGWTSAPFRQAADALRLSGQRVSLSCWPDAQVVVDAGAGHA
                     DVVGDGAAPAEQPHEVSSESIPAVQPAPPGERGLPDPQVPELAEGVVEVDATPADVAS
                     ELEQQPAIELTELNLDAGDDPDAGSGLEVIDLSVSRWGPISETPKPQGLASGEPTQPP
                     VELELPSAVDVQMPVAFWPATETAESIPPVGGMVGDEAALPEATLVSPEGLSAPQEGA
                     EDGHTADVAAQDAELYRQIGDLRVSTALYNVYLNEADEWSRQLATDLSEWALELDRPV
                     PDAAV"
     assembly_gap    3706..3805
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3806..6257
                     /locus_tag="PKN51_11540"
     CDS             <3806..6257
                     /locus_tag="PKN51_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT85772.1"
                     /translation="DEAQPPVVTRDSLQSLRALGDDIDVADVIDPDLFPIFEEEAEEL
                     LPQMGTALRRWVAHPDDREARAQALRALHTLKGSARLAGALRLGELAHRTESAVETLG
                     LDQLVSADLEPLLDHFDELQAGFRRLQAGEQGVETSQVASVPDVATAEDARPAEEPSA
                     ETGQGESRVVVPTVTRGDIASPAALSLVSARAAASQAVRVRSQLLDRLVMQAGEVATS
                     RARLEAEIGQLRGLLGELTGNLDRLRGQLRDVELQAETQMQSRLALSRDAQQNFDPLE
                     FDRFTRMQELTRMMAESVNDVATVQRSLQRAVEGSEDNLVAQARQSRELQRELLRTRM
                     VEFDSIAERLYRVVRQASKETGKQVRLDIVGGATEIDRGILERMTPAFEHLVRNCVVH
                     GIEPQAERSARGKEATGLISIQLQQAGNDVSVAFRDDGAGLNLSRIRERALQLGIVAA
                     DRELSDAEVANLVFMPGFSTASEVTELAGRGVGMDVVRSEVQALGGRIETQTTEGQGA
                     AFRLVLPLTTAVTHVVMLRCGALAIGVPSNVVEIVQRASAKDLSRAYETGHYRYGDES
                     LLFFWLGALLQDSPHSSEPPGRTLPVVVLRSADQRVAVHVDEVLGNQEVVVKALGPQL
                     SGLPGLVAITALASGAVALIYNPVALASVYGVQARAMVELPQAAPAEVPVPQGPVPTP
                     ALAPSGIPLVLVVDDSITVRRVTQRLLQREGYRVALAADGLQGLERLQAERPAVVLSD
                     IEMPRMDGFDFVRNIRADEALRDLPVIMITSRIAEKHREHARELGVDHYLGKPYSEDE
                     LLGLIRNYTRAAAPVGAA"
     gene            6300..6899
                     /locus_tag="PKN51_11545"
     CDS             6300..6899
                     /locus_tag="PKN51_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNT85773.1"
                     /translation="MADDAQPINLTNHFLIAMPGMVDGTFSRSVVYVCEHSQRGALGL
                     IINKPGDISLADLFERVDLPLSRPELGAQPVFQGGPLQTERGFVLHDPIVAEGLAPDQ
                     TVYASTLAVPGGLEMTTSKDVLEALSSGAGPRRVLVTLGYASWGEGQLESEIARNSWL
                     TVDADADLIFEAPIAQRYDLALRRLGLEAWMLSPDIGHA"
     gene            6896..7315
                     /gene="ruvX"
                     /locus_tag="PKN51_11550"
     CDS             6896..7315
                     /gene="ruvX"
                     /locus_tag="PKN51_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNT85774.1"
                     /translation="MSASASAPEVPPHLGSFLAFDFGLKRTGVAVGNRLTRSATPQPT
                     IRAEGQARWAPLAARIAEWQPDALVVGVPCHPDGAAHDNTRRAQRFARQLRGRFGLPV
                     YEVDERYSTTEAHAAGARDADAGAAVVILEQFLRSLA"
     gene            7312..7812
                     /gene="pyrR"
                     /locus_tag="PKN51_11555"
     CDS             7312..7812
                     /gene="pyrR"
                     /locus_tag="PKN51_11555"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNT85775.1"
                     /translation="MSALALDAEALYAELLRGVRSLVNPQTRLVGIVSGGRWLAERLQ
                     SDLGLAAPAGAISSAMHRDDFAKRGLAASGQTVLPFDVDGADILLLDDVLYTGRTLRA
                     VLNELFDYGRPQRVRLAVLVDRGGRQLPVAAEFAAARVALPADQSLELARSEDGRFSF
                     RVEQRG"
     gene            7815..>8005
                     /gene="pyrB"
                     /locus_tag="PKN51_11560"
     CDS             7815..>8005
                     /gene="pyrB"
                     /locus_tag="PKN51_11560"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015580.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNT85776.1"
                     /translation="MLERRNPQLNRHGELIHLLSTEGLSRELLTHILDTAAQFVSVSD
                     REVKKVPLLRGKSVFNLFF"
BASE COUNT         1227 a         2569 c         2845 g         1264 t
ORIGIN      
        1 gcgacgcgcc agatcgcggc gctggtgaag gcgattcaga ccgataccca ggacgcggtg
       61 gcggccatgg agcgctccac ccagggtgtg gtcgagggag cgcgtctgtc cgacaacgcg
      121 ggcaccgcac tgtcggagat cgaccgcgtg tcgcgtcagc tggccgagct gatcgaacgg
      181 atttctgctt ctgcctccaa ggaggccgat caggccaacg aggttgccgg caacatccag
      241 cacatcttcg cggtgaccga acagacgggg gaaggtacac gcaccacggc cacccaggtg
      301 cgcgaactgg ctcgcatggc cgacgaactg cgtcagtcgg tggcgcgttt caagatcgcc
      361 tgaccgatcg cgggcatggg gcggcggatc aggttccggc gcgcgcctgt acgagactgc
      421 tactggagtg cacgacatca tggtgatgag tcccgcagcc gaaatgcgcg acagtggcgc
      481 gtctgccgtc agggatctgg ggccgctggc ctgggtgctg gacgaaaccc gcaggtcgat
      541 cgaagcggcc agcaagtcgc tcaagcgttt tgcgcgtgaa accgatgcgg cgcgcggcat
      601 cgatctgggg tcggtggacg ccagtccgct gcgcatggcg cgccagcagc ttcaccaggt
      661 ggctggcgcg ctggacatgg tcgaccagtc ggtggcggca ctgatggtgc gtgggatgga
      721 gggtgcagtt cagcagtttg tgcagcggcc cgacaaatgc accgaggaaa ccgcacaggc
      781 ccttgagcgc gccggtttcg cgctcatgga atacctggaa gcccagatgg ggtcgcaccc
      841 acgctcggcg gttgggctgt ttccgcaata ccgccgagtc caggaactgg cgggcgccga
      901 tcgcatccat ccggcggatc tgtggtcggt gccctggagt tgggccgatc cggtggtgcc
      961 tgcatcggcg cagccgttgt cctacgaccc cgaagtgcgc caccgactgg atcagcgcgt
     1021 actcaagatc ttgcggcatc ctgctgccca cgcggccgac gaactcgccg tcatcagcct
     1081 gagcctcgcg gccggccaga tgcagcggca ggcgaccgtg ttctggctgc tgtctgcagg
     1141 cttcttcgag gccctggggc gtggattgat tgtcaaggac gtctatgcca agcgggcggc
     1201 gtcacgcatc ctgatgcaat acacctcgct ggcacgcggt ggtcaggtgg tgtccgagcg
     1261 attggcgcat gacttgctgt tcttctgttc gcaggccagg ttcgatgctg atgcgaacac
     1321 cccggcactg caggccgtgc gcaaggcttg gtcgcttgat gcgcagcagg cgacgtcgta
     1381 cgagcaggcc atgtttggac tttacgaccc ggcgatactg gcccaggcgc gtcgccggat
     1441 cgagtcggtc aaggaaaact ggtcggccct gaccggcggt gatctggccc gcatgaaggc
     1501 gtgcgtcgac cagttcagcc tggtggccga gtcgctcgac aagctgcacg agcagggatc
     1561 ggtgcttggt gaggtattga accgagtggc ccagcagacc gcgcaatcgg ggcgacctcc
     1621 tggcccggaa ctggcgatgg aaaccgcaac cgcggttttg tttctggagg cttcgttcgc
     1681 cgatttccac ccgggcgatg ccatgttttc cacccgcctg gcccagttgg ccgcgcggct
     1741 cgatgcggtt cgtgccggcg catcagcgcc accgctggag ccgtggatgg aggagctgta
     1801 ccgcaacgtc agcgatcgcc agaccatggg cacggtggtg ggcgagttgc gcaccaccct
     1861 gggtgaggtg gagcatctgc tggatcagtt cttccgtcaa cccgctgacc gcagcacgct
     1921 ggtgtcggtg ccgggcctga tggggcagat gcgtggggtg ctgtcggtgc tgggcctgga
     1981 tcaggctgcc ctggcagtgg gccgcatgcg cgatctggtc gacgaactgc tggccgcgca
     2041 gcccgacacc aagttggccg acagcgcgcc ttcgttcgaa aagctcgggg acaccctggg
     2101 cgcgttgggg tttcagatcg acatgctgag ctaccaaccg gtgctggcac gcaagctgtt
     2161 cgtgtatgac gccgatgccg gcgaactcag gtatcggtca ggccgcgccg caactcaggt
     2221 cacgacggag cagaattcgg tggcctcggc caccaccgac atcgatctgc tgcttgacgc
     2281 cggcaccgaa ctgacggtgc cgacatcggg gcccggctcc ctggtggacg aatcgctgcc
     2341 atccgaaacc atctctggcc agcttgacga gatcgccacc atggcagccc tggccgagca
     2401 gcgggctctt gccgacgcgg cgcagcgcgc tgccgacgcg gcgcgccgtc acgacgaagc
     2461 tggcctgagc ggcgcgctgg atgccctgca ggcactcacc gctgcatcga cgccgcagcc
     2521 cacgtccacg accgtcgaag gcggcggtgc ggatgatgat gatctgcagg ccattttcct
     2581 tgaggaagcg cagcaggtga tggacaccgg gtgcgaagtc gtgcaggcgc tgttgaccgc
     2641 gcccggtgac atggagctgc agaccgcctt gcgtcgtgcg ttccataccc tgaagggcag
     2701 ttctcgcatg gtcgggttga ccgagtttgg cgaggccgcc tgggccatgg agcagctgtt
     2761 caatgcctgg ctgcccgagc agaagccggt gaccgaggac atgcgagggt tggcacttga
     2821 agcactgcaa ggcctgcacg cctggacgga agccatcgct ggtcggaacg accacggttg
     2881 gaccagcgcg ccgttccggc aggcagccga tgccctgcga ctcagcggtc aacgtgtgtc
     2941 gctgagctgc tggcccgatg ctcaggtcgt tgtggacgca ggcgccggcc atgcggatgt
     3001 ggtcggggac ggcgccgcgc ctgccgagca gccgcatgag gtgtcgtcgg agtccattcc
     3061 ggcggtgcaa ccggcgccac ctggagagcg gggtctgccg gatccacagg tgcccgagtt
     3121 ggctgagggt gtcgtcgaag ttgacgccac gccagcggat gtggcttcgg agttggagca
     3181 gcagcccgcg atcgagctga cggaactgaa tctcgatgct ggtgacgacc ccgatgccgg
     3241 ttccggcctc gaagtcatcg acctgagcgt gagtcgatgg ggcccgatat ccgaaacacc
     3301 caagccgcag gggctggcga gcggcgaacc cacgcaacca cccgttgagc tcgagctccc
     3361 cagcgcggtc gacgtgcaga tgcccgtggc attctggcca gccaccgaga cggcggaatc
     3421 gatcccgccg gtgggtggga tggtggggga tgaagcggcg ctgccggagg cgacgctggt
     3481 cagcccggag gggctgagcg cgccacagga gggtgccgaa gatgggcaca ctgccgacgt
     3541 cgctgcgcag gacgccgagc tctaccgtca gatcggggac ctgcgcgtca gcactgcgct
     3601 ctacaacgtg tacctgaatg aggccgacga gtggtcgcgg caactggcaa ccgacctgtc
     3661 ggagtgggcg ctggagcttg atcggccggt gcccgacgct gccgtnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnagacg aagctcagcc gccggtggtc acccgggatt
     3841 cgttgcagtc gctgcgtgcc ctgggtgacg atatcgacgt cgccgacgtg atcgatcccg
     3901 acctgttccc catctttgag gaagaggcgg aagaacttct gccgcagatg ggcacggcct
     3961 tgcgtcgctg ggtggcgcac ccggacgatc gtgaggcacg tgctcaggcc ctgcgtgccc
     4021 tgcacacgct caagggcagc gcacggctcg ccggcgcgtt gcggctgggc gagctggcac
     4081 accggacgga atcggccgtc gagaccctgg ggctggacca gctcgtttcc gccgatctgg
     4141 agccgctgct ggatcacttc gatgagttgc aggctgggtt ccgccggctg caggcgggcg
     4201 agcagggcgt ggagaccagc caggtggcgt cggtgccgga tgttgccacg gcggaagatg
     4261 caaggccggc ggaggagccg tctgccgaga cgggtcaggg cgagtcgcgc gtcgtcgtgc
     4321 cgacggtcac acggggggac atcgcttcgc cggccgcgct gtcgctggtg tcggcacgcg
     4381 ccgccgccag ccaggcggtg cgggtgcgct cacagctgct cgatcggctg gtgatgcagg
     4441 ccggcgaggt ggccacctcg cgcgcccggc tggaagccga aatcggccag ttgcgcgggc
     4501 tgctggggga gctgaccggc aacctcgacc gcctgcgtgg ccagttgcgc gacgtcgagc
     4561 tgcaggccga gacgcagatg cagtcgcggc tggcgctgtc ccgggacgcg cagcagaact
     4621 tcgatccgct ggagttcgac cgattcacgc gcatgcagga actcacccgc atgatggccg
     4681 agtcggtcaa cgacgtcgcc accgtgcagc gcagcctgca gcgcgccgtc gagggcagcg
     4741 aagacaacct ggtggcgcag gcgcgccagt cgcgcgagtt gcagcgcgag ctgctgcgca
     4801 cccgcatggt ggagttcgac agcattgccg aacgcctgta tcgggtggtg cgccaggcca
     4861 gcaaggaaac cggcaagcag gtgcggctcg acatcgtggg cggcgcgacc gagatcgatc
     4921 gcggcatcct ggagcgcatg acgccggcct tcgagcacct ggtgcgcaac tgcgtggtgc
     4981 atggcatcga gccgcaggcc gagcgcagcg cgcgcggcaa ggaagccacc ggactgatca
     5041 gcatccagct gcagcaggcc ggcaacgacg tgtcggtggc gttccgcgat gacggcgccg
     5101 gcctcaacct gtcgcgcatc cgcgaacgcg ctctgcagtt gggcatcgtg gcggccgatc
     5161 gtgaactgag cgatgccgag gtggccaacc tggtgttcat gccgggcttt tccaccgcca
     5221 gcgaagtcac tgaactggcc ggtcgcggcg tcggcatgga cgtggtgcgc tcggaggtcc
     5281 aggccctggg cggccggatc gagacgcaga ccaccgaggg ccagggggcg gcgttccggc
     5341 tggtgctgcc gttgaccacc gccgtcaccc acgtggtcat gctgcgctgc ggcgcgctgg
     5401 cgatcggcgt gccgtccaac gtggtcgaga tcgtgcagcg cgcatcggcc aaggacctgt
     5461 cgcgggccta cgagacgggg cactaccgct atggcgacga gtcgctgctg ttcttctggc
     5521 tgggcgcgct gctgcaggat tcgccccaca gcagcgagcc gcccgggcgg accttgccgg
     5581 tggtggtgct gcgttcggcc gatcagcgtg tggcggtgca cgtcgacgag gtgctgggca
     5641 accaggaagt ggtggtcaag gccctggggc cccagctgtc gggcctgcct ggcctggtgg
     5701 ccatcaccgc cctggcttcc ggtgcggtgg cgctgatcta caacccggtg gcgctggcca
     5761 gcgtgtacgg cgttcaggca cgcgcgatgg tcgagctgcc gcaggcggcg cccgccgagg
     5821 tgccggtgcc gcaagggccg gtgccgacac ccgcactggc gccttcgggc attcccctgg
     5881 tgctggtggt cgacgactcg atcaccgtgc gccgcgtcac ccagcgcctg ctgcagcgcg
     5941 aaggctaccg cgtggccttg gccgccgatg gtctgcaggg gctggagcgc ctgcaggccg
     6001 agcggccggc ggtggtgctg tccgacatcg agatgccgcg catggacggc ttcgacttcg
     6061 tgcgcaacat tcgtgccgac gaagcgctgc gcgacctgcc ggtaatcatg atcacctcgc
     6121 gcattgccga gaagcaccgc gaacacgcgc gcgaactggg cgtcgaccac tacctcggca
     6181 agccgtactc cgaggacgaa ctgctggggc tgatccgcaa ctacacccgc gcggcggctc
     6241 ccgtcggcgc cgcctgaggc cacgggtgcg gccccggcag cggccggtaa aatcgctgca
     6301 tggccgatga cgcgcagccg atcaacctga cgaatcactt cctcatcgcc atgccgggca
     6361 tggtcgatgg gacgttttcg cgcagcgtgg tctacgtgtg cgagcacagc cagcgcggcg
     6421 ccctgggcct gatcatcaac aagccgggcg acatcagcct ggccgacctg ttcgagcggg
     6481 tcgatctgcc cctgtcgcgc cccgaactgg gcgcgcagcc ggtgtttcag ggcggccctt
     6541 tgcagaccga gcgcggcttc gtgctgcacg acccgatcgt ggccgagggt ctggcgccgg
     6601 accagacggt gtacgcctcc acgctggcgg tgcccggcgg gctggagatg accacgtcca
     6661 aggacgtgct ggaggccctg tcctccggtg ccgggccgcg gcgcgtgctg gtcacgctgg
     6721 gctatgcgtc ctggggcgag ggtcagctcg aatccgagat cgcgcgcaac agctggctga
     6781 cggtggacgc cgacgccgac ctgatcttcg aagcaccgat cgcgcagcgc tacgacctgg
     6841 ccctgcggcg gctgggactg gaggcgtgga tgctgtcacc ggacatcggt cacgcatgag
     6901 cgccagcgcg tccgcgcccg aggtgccgcc ccatctgggc agctttctgg ccttcgactt
     6961 cggcctgaaa cgcaccggcg tggcggtcgg caaccggctc acgcgcagcg ccacgccgca
     7021 acccaccatc cgtgccgaag gccaggcgcg ctgggcgccg ctggcggcgc gcatcgccga
     7081 atggcagccc gatgcgctgg tggtgggcgt gccctgtcac cccgacggcg cggcacacga
     7141 caacacacgc cgtgcgcagc gcttcgcgcg ccagctgcgt gggcgcttcg ggctgccggt
     7201 gtacgaggtg gacgagcgct acagcaccac cgaggcccat gcggccggcg cccgtgacgc
     7261 cgacgccggt gcggcggtgg tcatcctgga gcagtttctg aggagtcttg catgagtgca
     7321 ctggcgctgg acgccgaagc cctgtacgcc gagctgctgc gcggcgtgcg gtccctggtc
     7381 aacccgcaga ctcggctggt cggtatcgtc tcgggcgggc ggtggctggc cgagcgcctg
     7441 cagtccgatc tggggctggc cgcccccgcg ggggcgattt cctcggccat gcaccgcgac
     7501 gacttcgcca agcgcggcct ggccgccagt ggccagaccg tgctgccgtt cgacgtcgat
     7561 ggcgccgaca tcctgttgct cgacgacgtg ctctacaccg gacgcaccct gcgcgccgtg
     7621 ctcaacgagc tgttcgacta cggccggccg cagcgcgtgc ggctggcggt gctggtcgac
     7681 cgcggcggcc gccagctgcc ggtggcggcc gaattcgccg ccgcgcgggt ggcgttgccg
     7741 gccgaccagt cgctggagct ggcgcgcagc gaggacggcc gcttcagttt ccgtgtcgaa
     7801 caacgaggct gaccatgctg gagcggcgca acccccaact caaccgccac ggcgagctga
     7861 tccacctgct gtccaccgaa gggctgtcgc gtgagctgct cacccacatc ctggacaccg
     7921 cggcgcagtt cgtcagcgtc agcgaccgcg aggtcaagaa ggtgccgctg ctgcgcggca
     7981 agagcgtgtt caacctgttc ttcga
//
LOCUS       DAOQTZ010000114         7928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_10091_length_7928_cov_14.119776, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000114 DAOQTZ010000000
VERSION     DAOQTZ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10091_length_7928_cov_14.119776"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /gene="ruvB"
                     /locus_tag="PKN51_11565"
     CDS             complement(<1..989)
                     /gene="ruvB"
                     /locus_tag="PKN51_11565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNT85777.1"
                     /translation="MSIHTDDFALPPGAAAPRLVSGAAASTREEALERALRPKLLDEY
                     VGQAKAREQLEIFIGAARKRGEALDHVLLFGPPGLGKTTLSHIIAHELGVQLRQTSGP
                     VLEKPKDLAAILTSLEKHDVLFIDEIHRLSPVVEEILYPALEDYQIDILIGEGPAARS
                     IKLDVQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPDELARIVRRSAGLLQVEAD
                     AEGAFEIARRSRGTPRIANRLLRRVRDYADVKGQGRITQDIAHAALALLDVDPEGFDV
                     MDRKLLEAVIQRFDGGPVGLDNIAASIGEEPGTIEDVIEPYLIQQGYLQRTPRG"
     gene            complement(999..1577)
                     /gene="ruvA"
                     /locus_tag="PKN51_11570"
     CDS             complement(999..1577)
                     /gene="ruvA"
                     /locus_tag="PKN51_11570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNT85778.1"
                     /translation="MIGRLAGLLAEKTPPQILIDCQGVGYEVWVPMSSFYQLPALGER
                     VTLLTHFVVREDAQLLYGFVTETERAAFRELIRISGIGPRMALAVLSGMSVAELAQAI
                     AAQQAGRLTKVPGIGRKTAERLLLELKGRLGPDLGAPGAAAPASDSQTDILQALLALG
                     YSDKEAAAALQALPPDVGVSEGIRLALKALAK"
     gene            complement(1769..2428)
                     /locus_tag="PKN51_11575"
     CDS             complement(1769..2428)
                     /locus_tag="PKN51_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT85779.1"
                     /translation="MKAVLVIEDLPDIRSWLVDTVRVAFPDAEVTAIGRCGDGIDALA
                     RQPFDVVLTDLELPDGSGVSVIRALRQQQPMALSVVVTIHDDDEHLFPALQAGAFGYL
                     LKEASRQELVAQLQRIADGEPPLSPPIARRMLAYFSQGGPRVVDVTRFDPHEVALNGR
                     ETDILQRVAKGYTLPEIAGQLNLSRHTVADYVKQIYRKLNVSSRAEAALEAARRGLVR
                     P"
     gene            complement(2562..4916)
                     /locus_tag="PKN51_11580"
     CDS             complement(2562..4916)
                     /locus_tag="PKN51_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85780.1"
                     /translation="MNPSDPSPTLPSALYTESDLPPTLPGLLDTESGLSGSWRTPLDQ
                     LDLLERERPAQATPWLYRQAILLAGLWAVLLTTLALALATRQPWLGLRLAAQDGQVQV
                     VDVVPGSPAAVAGVHVGAQLQALATADGTQAMTLQLDDLRANPDELASYADWGRFYER
                     QDRLARLLGDASLRLTMVAHGAQEEALLLRPAPTRPPGSLPPLFWTLMVLQAAIMLMA
                     GWVITVYPPQPAAPRSAVGAMAISLVLAGVAVFGTRELALPSTLFHALVVLQHAGSLV
                     AALAVASILSHYPSRLPEMGGWRVWVVAGLFLLWALADAGWWLPSPNWASRAMLLAAV
                     VVVTVQAVRQWQLHSGGDSVARDEARTQALIWIPAAFCLFGLREGALLLGWAPPLSQA
                     WLYGLVVVAVAGRAVTHREVRVFELDDWTRQVLLTWGAGVTILLCYRLLQASRWLSDE
                     LAMLVSVLGLGLVYVLLTRRIWGRGILRRSQTQRELTSAILALGLTAPEDRGPLWMAL
                     LENTFEARVSLADGAQPYRVTTVLDGGRTLWVPPVAGLPGVLLGQRDRGRPLFARSDR
                     RMAARLSALAAQIIHARDAYVRGASEERLRIADDLHDDLGAKLLSLAHAGEQGEAGAD
                     VARLAREALEEMRLSVRHLKAQPMPAADVLADWRAETVARLSAAGVQVDWDAQLSGRV
                     APLPMRTVAHLTRVLREAVSNIIHHSGASYCRVLLQLSPTALHLDVEDNGQGMGLPGA
                     RGGAGGLSSIERRVRRLGGTHRFGSGALGGTLLMVRVPLESPHS"
     gene            4942..5901
                     /locus_tag="PKN51_11585"
     CDS             4942..5901
                     /locus_tag="PKN51_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT85781.1"
                     /translation="MILRHTFTPSNNTRLAHLCGPTDEHLRTIEAGLQVSIAHRHEQF
                     RIDGARKSAERALTVLQALYELADRPIRTETVQLMLAGDGEPLAEDAGGAHRLVTRRG
                     DLKARTPNQSVYLEHIAQHDITFGIGPAGTGKTYLAVACAVDALERQSVQRIVLTRPA
                     VEAGERLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVAKAFERAQLEIAPLAFMRGR
                     TLNHAFVILDEAQNTTPEQMKMFLTRIGFGSKCVVTGDVSQIDLPKGATSGLIDAERV
                     LRRVKGLAFTRFTSADVVRHPLVARIVDAYDAAGRPARTPKNS"
     gene            5960..6427
                     /gene="ybeY"
                     /locus_tag="PKN51_11590"
     CDS             5960..6427
                     /gene="ybeY"
                     /locus_tag="PKN51_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNT85782.1"
                     /translation="MATPSLRLSLQFARSPEMAPHRAALPRAQVLRWLRAALGADVRL
                     AEIAVRVVGADEGRQLNRDYRGKDYATNVLTFDYSGAPQLAADLVLCAPVVAREAAEL
                     ATPLAQHYAHLLVHGALHAQGWDHETSEQEAEAMEARETAILAALGMPDPYAR"
     gene            complement(6568..7884)
                     /locus_tag="PKN51_11595"
     CDS             complement(6568..7884)
                     /locus_tag="PKN51_11595"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HNT85783.1"
                     /translation="MIRLFRSTHLPEETTVKLFRSIQRARGAAAAVGAALLLLTTSAH
                     ADFVNGGFEQSPDFTGWTTPGFAIPGAIPTFPPTQWSHLGLTTGTSQSAVLNGPGNAA
                     ATDGNLTWTNRVARVHEEFSNGDGNTASAIEQVLTVAAGDIEADGKVHIRFNAAPVLE
                     DPGHAPDEQPYFFIEVTNQHGTSLYHTFNYSNEPGVPWVLNSGRSHRYTNWQAFDIPV
                     TAAVGDQLTLRLLAAGCGQGGHAGALYVNDVRTQAAVQGPSLWVSAAGPATICLNRGG
                     TTNVTYTYTYRNNGTDPMYGVTASVSMPVTSDSTPVTTTFVSITNPTFGGGTCTAPAS
                     PGNPATCSIGTLQPGQQGTFQMTVQIPAGAIGPTLNNGTYSLSGATSAQGGTVVTQLG
                     PLVRTAMSENCAVNVAPVPTLGEWAMMLLASLMAAFGWVMMRRRMG"
BASE COUNT         1250 a         2857 c         2718 g         1103 t
ORIGIN      
        1 ccgcgcggcg tgcgctgcag gtagccctgc tggatcaggt agggctcgat cacgtcctcg
       61 atggtgcccg gctcctcgcc gatgctggcg gcgatgttgt ccaaccccac cgggccgccg
      121 tcgaagcgct ggatcaccgc ttccagcagc ttgcggtcca tgacgtcgaa gccctcgggg
      181 tcgacgtcca gcagcgccag cgcggcgtgc gcgatgtcct gcgtgatgcg gccctggccc
      241 ttgacgtcgg cgtagtcgcg cacgcggcgc agcaggcggt tggcgatgcg cggcgtgccg
      301 cgcgagcggc gcgcgatctc gaacgcgcct tcggcgtcgg cctcgacctg cagcaggccg
      361 gccgagcgcc tgacgatgcg cgccagctcg tcgggggtgt agaactccag ccgcgccacg
      421 atgccaaagc ggtcgcgcag cgggttggtg agcatgccgg cgcgcgtggt ggcgcccacc
      481 agggtgaagg gctgcacgtc cagcttgatc gagcgcgccg ccgggccttc gccgatcagg
      541 atgtcgatct ggtagtcctc cagcgcgggg tagaggattt cctcgaccac cggcgacagg
      601 cggtggatct cgtcgatgaa caacacgtcg tgcttttcca ggctggtcag gatggcggcc
      661 aggtccttgg gcttttccag caccgggccg ctggtctggc gcagctgcac gcccagctcg
      721 tgcgcgatga tgtggctgag cgtggtcttg cccagccccg gcgggccgaa cagcagcaca
      781 tggtccagcg cctcgccgcg cttgcgggcg gcgccgatga agatctcgag ctgctcgcgc
      841 gccttggcct ggcctacgta ctcgtccagc agcttgggcc gcagcgcgcg ctccagcgcc
      901 tcttctcgcg tcgaggcggc agcgcccgac accaggcgcg gcgccgcggc gccgggcggc
      961 agggcaaagt cgtcggtgtg gatgctcatg cggcgtgatt atttcgccaa cgccttgagc
     1021 gccagccgga tgccctcgct cacgcccacg tcgggcggca gcgcctgcag ggcggcggcg
     1081 gcttccttgt cgctgtagcc cagcgccagc agcgcctgca ggatgtcggt ctggctgtcg
     1141 ctggcggggg cggcggcgcc gggcgcaccc aggtcggggc cgagccggcc cttgagctcc
     1201 agcagcagcc gctcggcggt cttcctgccg atgcccggca ccttggtcag gcggcccgcc
     1261 tgctgcgccg cgatggcctg cgccagctcg gccacgctca tacccgacag caccgccagc
     1321 gccatgcgcg ggccgatgcc gctgatgcgg atcagctcgc ggaaggcggc ccgctcggtc
     1381 tcggtgacga agccgtagag cagctgcgcg tcctcgcgca ccacgaaatg cgtcagcagc
     1441 gtgacccgct cacccagcgc cggcagctgg tagaagctgc tcatgggcac ccacacctcg
     1501 tagccgacgc cctggcagtc gatcaggatc tggggtgggg ttttctcggc cagcaggccg
     1561 gcaaggcgtc cgatcacggc gggggctttc agaagatcca cagagcgcga ccaccacgcc
     1621 cagcgtcaat gataaatcag ccgaaacaag ccgtccggcc tgcgccagca gctctcattt
     1681 caagagcatt cgggaaccac agggagcgcc ggaacgcgcc ccaccgaaac ctgcggtgac
     1741 aggtggcagg cgggcaccgg gcaaccagtc agggccgcac caggccgcgg cgcgcggcct
     1801 ccagtgccgc ttcggcgcgc gaggacacgt tgagcttgcg gtagatctgc ttgacgtagt
     1861 cggccaccgt gtggcgcgac aggttcagct ggcccgcgat ttcgggcagg gtgtagccct
     1921 tggccacgcg ctgcaggatg tcggtctcgc gtccgttcag cgccacttcg tgagggtcga
     1981 aacgggtcac gtcgaccacg cgcgggccgc cctggctgaa gtaggccagc atgcgccggg
     2041 cgatcggagg cgacagcggt ggctcgccgt cggcgatgcg ctgcagctgc gccaccagct
     2101 cctggcgcga cgcttccttc agcaggtagc cgaaggcgcc ggcctgcagc gcgggaaaca
     2161 gatgttcgtc gtcgtcgtgg atggtgacca ccaccgacaa cgccatgggc tgctgctggc
     2221 gcagcgcccg gatcaccgag acgccgctgc cgtcgggcag ttccagatcg gtcagaacga
     2281 cgtcgaacgg ctgccgcgcc agcgcatcga tgccatcacc acaacggcct atcgccgtca
     2341 cctcggcatc ggggaacgca acgcgcacgg tgtccaccag ccaggaacgg atgtccggca
     2401 gatcctcgat cacaagcacg gctttcatga cttggccatg ctaagccggc ggcggccgga
     2461 tgcttcggcc cggcatccac gaacttgcgg gaaatgcgga atatgtcgcc accggcgcac
     2521 cgccaccgcc ctgggccggc ttgcggcaga ggccgcgaga gtcagctgtg cggcgactcc
     2581 agcggcaccc gcaccatcag cagcgtgccg cccaatgcgc cgctgccgaa gcggtgcgtg
     2641 ccgcccaggc ggcgcacgcg gcgctcgatg ctcgacaggc cgccggcgcc gccccgagct
     2701 cccggcaacc ccatgccctg gccgttgtct tccacgtcca ggtggagggc ggtcggactg
     2761 agctgcaaca gcacgcggca ataactggca ccgctgtggt gaatgatgtt ggacacggcc
     2821 tcgcgcagca cccgcgtcaa gtgggcgacg gtgcgcatgg gcagcggggc cacccgcccc
     2881 gacagctggg cgtcccagtc gacctgcacg ccggcggcgg acagccgcgc cacggtttca
     2941 gcgcgccagt cggccagcac gtcggccgcc ggcatcggct gcgccttcag gtggcgcacc
     3001 gacagccgca tttcctccag cgcctcgcgc gccaaccggg ccacgtcggc gccggcctcg
     3061 ccctgctcgc ccgcgtgcgc cagcgacagc agcttggccc ccaggtcgtc gtgcaggtcg
     3121 tcggcgatgc gcaggcgctc ttcgctggcg ccgcgcacgt aggcgtcgcg ggcgtggatg
     3181 atctgcgccg ccagcgccga caaccgggcc gccatgcgcc ggtcgctgcg ggcgaacagc
     3241 ggccggccgc ggtcgcgctg acccagcagc acccccggca gaccggccac cggcggcacc
     3301 cacagcgtcc ggccgccgtc cagcacggtg gtcactcggt agggttgcgc gccgtcggcc
     3361 aggctcacgc gcgcctcgaa ggtgttttcc agcaaggcca tccacagcgg accccggtcc
     3421 tccggcgccg tcaacccgag cgccaggatg gcgctggtga gctcgcgctg cgtctggctg
     3481 cgccgcagaa tgccgcgccc ccagatgcgc cgcgtcagca gcacatagac caggcccaga
     3541 cccagcaccg acaccagcat ggccagctcg tcgctgagcc agcggctcgc ctgcagcaac
     3601 cgatagcaca gcaagatggt gacgcccgcg ccccaggtca gcagcacctg gcgcgtccag
     3661 tcgtccagct cgaacacccg cacctcacgg tgcgtgaccg cgcgcccggc cactgccacc
     3721 accaccagcc cgtacagcca ggcctgcgac agcggcggcg cccaccccag cagcaaagcc
     3781 ccctcgcgca ggccgaacag gcagaacgca gccgggatcc agatcagcgc ctgcgtgcgc
     3841 gcctcgtcgc gcgccaccga atcgcccccc gaatggagct gccactggcg caccgcctgt
     3901 accgtaacca ccaccaccgc cgccagcagc atggcgcggc tggcccagtt gggcgacggc
     3961 aaccaccagc cggcatcggc cagcgcccac agcaggaaca ggccggccac cacccagacg
     4021 cgccagccgc ccatttcggg caagcgactc gggtagtgcg acagaatgct cgccacggcg
     4081 agtgcggcga ccaggctgcc ggcatgctgc agcaccacca gggcatggaa cagggtcgag
     4141 ggcagcgcca gctcccgcgt accgaacacc gccacgccgg ccagcaccag cgagatcgcc
     4201 atcgccccca cggcgctgcg cggggcggcc ggctggggtg ggtacacggt gatcacccag
     4261 ccggccatca gcatgatggc cgcctgcagc accatcaggg tccagaacag cggtggcagg
     4321 ctgcccggcg gccgcgtcgg cgccgggcgc agcaacaagg cctcttcctg cgcgccatgc
     4381 gccaccatcg tcagccgcaa cgacgcgtcg ccgagcaggc gcgccagccg gtcctgccgc
     4441 tcataaaacc ggccccagtc ggcataactg gccaactcgt ccgggttggc ccgcaagtca
     4501 tcaagctgca gcgtcatggc ttgcgttcca tcggcggtcg cgagtgcctg cagctgcgcc
     4561 ccgacgtgca cgccggccac cgccgccggg ctgcccggca ccacgtccac gacctgaacc
     4621 tgcccgtcct gcgccgccag ccgcaggccc agccacggct ggcgagtggc cagcgccagc
     4681 gccagtgtgg tcagcaacac cgcccacagg ccggccagca ggatggcctg gcggtacagc
     4741 cagggcgtcg cctgggccgg ccgttcgcgc tcgagcaggt ccagttgatc cagcggcgtg
     4801 cgccagctgc cggacaggcc ggactcggtg tccagcagcc ccggcagcgt gggcggcagg
     4861 tccgattcgg tgtagagcgc gctgggcagg gtgggtgaag gatcggacgg gttcacggtc
     4921 aggatggggg acaatccaag cgtgattctg cggcatacct tcaccccatc caacaacacc
     4981 cggctggccc acctgtgcgg cccgacggac gagcacctgc gcaccatcga agccggcctg
     5041 caggtgagca tcgcgcaccg gcacgagcag ttcaggatcg atggcgcccg caagtcggcc
     5101 gagcgcgccc tgaccgtgct gcaggcgctg tacgaactgg ccgaccgccc gatccgcacc
     5161 gagacggtgc agctgatgct ggccggcgac ggcgagccgc tggccgagga cgcaggcggc
     5221 gcccatcgcc tggtcacgcg ccgcggcgac ctgaaggcgc gcacgcccaa ccagagcgtg
     5281 tacctggagc acatcgccca gcacgacatc accttcggca tcggcccggc cggcaccggc
     5341 aagacctatc tggcggtggc ctgtgcggtc gacgcgctgg agcgccagtc ggtgcagcgc
     5401 atcgtgctga cgcgcccggc ggtcgaggcc ggcgagcgcc tgggctttct gccgggcgat
     5461 ctgacgcaga aggtcgaccc ctacctgcgc ccgctgtacg acgcgctgta cgacctgatg
     5521 ggcttcgacc gcgtggccaa ggccttcgag cgcgcgcaac tggaaatcgc gccgctggct
     5581 ttcatgcgtg ggcgcacgct gaaccacgcc ttcgtcatcc tggacgaggc gcaaaacacc
     5641 acgcccgagc agatgaagat gttcctgacc cgcatcggct tcggcagcaa atgcgtggtg
     5701 acgggcgacg tcagccagat cgacctgccc aagggcgcca ccagcggcct gatcgacgcc
     5761 gaacgggtgc tgcggcgcgt caagggcctg gccttcaccc gcttcaccag cgccgacgtg
     5821 gtgcgccacc cgctggtggc gcgcatcgtg gacgcctacg acgccgccgg gcggcctgcc
     5881 cgcaccccga aaaactcctg atttgatagc tgctggcgca cgtccaataa gcgccagagg
     5941 ccgatttgac ccgcatacca tggccacgcc ctcgcttcgc ctgtcgctgc agttcgcccg
     6001 ctcgcccgag atggcgccgc accgggccgc gctgccgcgc gcgcaggtcc tgcgctggct
     6061 gcgcgcggcg ctgggggcgg acgtgcggct ggccgaaatc gcggtgcggg tggtcggcgc
     6121 cgacgaaggc cgccagctca accgcgacta ccgcggcaag gactacgcca ccaacgtgct
     6181 gaccttcgac tacagcggcg cgccgcagct ggcggccgac ctggtgctgt gcgccccggt
     6241 ggtggcgcgc gaggcggccg aactggccac gccgctggcc cagcactacg cccacctgct
     6301 ggtgcacggc gccctgcacg cccagggctg ggaccacgaa accagcgaac aggaggccga
     6361 agccatggaa gcgcgcgaaa ccgccatcct ggcggcgctg ggcatgcccg acccctacgc
     6421 gcgctgacgc cggtggccgc gggcgaaacc ttgtccatcg ccgccgtcgt ggcgcaaaaa
     6481 aaggactgac gccagcaagg cgtcagtccc gcgctttggc ccaggggtgc cgccaggcac
     6541 cccggtctgc gccctggtgg agcgaaatca acccatgcgg cgacgcatca tgacccagcc
     6601 gaaggccgcc atcaggctgg ccagcagcat catggcccac tcacccagcg tgggaaccgg
     6661 ggccacgttg accgcgcagt tttcgctcat ggcggtgcgc accagcggcc ccagttgggt
     6721 caccacggtg ccgccctggg cactggtcgc gccactgagg ctgtaggtac cgttgttgag
     6781 cgtggggccg atggcccctg ccgggatctg caccgtcatc tggaacgtgc cctgctggcc
     6841 gggctgcagc gtaccgatac tgcaggtcgc cgggttgccc gggcttgcgg gcgccgtaca
     6901 ggtgccgcca ccgaacgtgg ggttggtgat gctgacgaac gtggtggtca ccggcgtgct
     6961 gtcggacgtg acgggcatgg acaccgaggc cgtcacaccg tacatcgggt ccgtcccgtt
     7021 gttgcggtag gtgtaggtgt aggtgacgtt ggtggtgccg ccccggttca gacagatcgt
     7081 ggccggcccg gccgcggaca cccacaggct ggggccttgg acggccgcct gggtgcggac
     7141 atcgttcacg tacagcgcgc cggcatgccc gccctggccg cagccggcgg ccagcaagcg
     7201 cagcgtcagt tggtcgccca ccgccgccgt caccggaatg tcgaacgcct gccagttggt
     7261 gtagcggtga gagcggcccg aattcagcac ccaggggaca cccggttcat tggagtagtt
     7321 gaacgtgtgg taaaggctgg tgccgtgctg gttggtgacc tcgatgaaga aatacggctg
     7381 ttcatcaggt gcgtgtcccg ggtcctccag caccggcgcc gcattgaagc ggatatgcac
     7441 cttgccatcc gcctcgatgt cgcccgccgc caccgtcagc acctgttcga tcgcgctggc
     7501 cgtgttgccg tcaccgttgc tgaactcttc gtgcacacgg gccacgcggt tggtccaggt
     7561 caggttgccg tcggtggcag ccgcattgcc cgggccgttc agcaccgccg actggctggt
     7621 gccggtggtc aggcccaggt ggctccactg ggtcggggga aacgtgggaa tggcaccagg
     7681 aatggcaaac ccaggtgtcg tccagccggt gaaatcggga gactgctcaa aaccgccgtt
     7741 gacgaagtcg gcgtgggccg acgtggtcag cagcagcagg gcggcgccga cggcggccgc
     7801 agcacccctg gcgcgctgaa tggaacgaaa aagcttcaca gtggtttcct ccggtagatg
     7861 ggtacttctg aataaacgaa tcacacgagc ctcgccagtt tatgccgtca cccgcgagca
     7921 tgtcgagc
//
LOCUS       DAOQTZ010000115         7889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_10151_length_7889_cov_15.651008, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000115 DAOQTZ010000000
VERSION     DAOQTZ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10151_length_7889_cov_15.651008"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1178
                     /locus_tag="PKN51_11600"
     CDS             60..1178
                     /locus_tag="PKN51_11600"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF002841.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNT85784.1"
                     /translation="MSRPTRKPQIPNFSSGPCAKRPGYDVARLDLSALGRSHRSSLGK
                     QLLGAACSETARILGLPAGYRVGIVPASDTGAVEMVLWSMLGARGVDMLAWESFGAGW
                     VTDVVKQLKLKDVTRHEAGYGELPDLSKVNFANDVVFTWNGTTSGVRVPDGDWIADDR
                     AGLTICDATSAVFAMALPWHKLDVVTYSWQKVLGGEGAHGMLILSPRAVERLESYSPP
                     WPLPKIFRMTSGGKLSEGIFQGDTINTPSMLCVADYLDALAWCERVGGVPGTIARSTA
                     NLKAIEDFVAAHPWISFLARRPETRSNTSVCLSLDLPAEQVKALVKLLEKEGVAYDIG
                     SYKDAPPGIRIWCGATVETADVQALLPWLAWAYEQVKA"
     gene            1317..2489
                     /locus_tag="PKN51_11605"
     CDS             1317..2489
                     /locus_tag="PKN51_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNT85785.1"
                     /translation="MFRIKTYNAISVKGLERFGRERYEVGHDIAHPDAVLLRSHKLHG
                     EALPASLLAVARAGAGVNNIPVADYGQQGIVVFNTPGANANAVKELVLAGMLLATRGI
                     LPGIAYAQSLAGMDDAAAMHALLEKEKSRFAGHELKGKTLGLVGLGAIGALVADMALA
                     LGMKVLGYDPVLSVDAAWRLSNAVQRMENLPALLAHADYVSLHVPAMDATRHLINAEA
                     LRHLRPSAVLLNFARETIVDPAAVLAALQAGRLGRYVCDFPEPGFAAEPRVIALPHIG
                     ASTEESEENCAVMAADQLIDFLEHGHIVNSVNYPALRMHRAPGSCRIAIANDNVAGVL
                     GHVLSALADAGVNVLDMSNRSREALAYNLIDVASAPEPAVIDAIRRVPHVIRVRTL"
     gene            complement(2506..3705)
                     /locus_tag="PKN51_11610"
     CDS             complement(2506..3705)
                     /locus_tag="PKN51_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNT85786.1"
                     /translation="MSRGFRARPWLGVLLALGASAALLGCGGSGDAGDARLSAEAQLG
                     EKIFSDVSLSASGRQSCATCHDAARGHAANNALPAQLGGPDGTLQGVRNTPGIRYLAS
                     NTPFFFDEEGTPTGGFFWDGRAATLAEQAAGPFLNPLEMANPDKASVITKLAAAPYAA
                     EFRRVYGDAIFSDVEGAYQRLTEALQRYQLEDVEFNAFTSKYDAYLRGTAQLSAQEAR
                     GLALFNEPTKGNCAACHPSAPGVQGALPLFTDFTYDNLGLPRNPALRANDDPAYHDLG
                     LCQRADLAGRDDLCGAFKVPSLRNVALRQALFHNGVFTSLRDAVRFYVLRDTQPELWY
                     PRRADGSVDKFNDLPAHYHASVNTSEAPYDRQPGDAPALNDAEIDDVVAFLRTLTDGY
                     RPPVEQP"
     gene            3867..4829
                     /locus_tag="PKN51_11615"
     CDS             3867..4829
                     /locus_tag="PKN51_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT85787.1"
                     /translation="MQRRSWLACLVLGWAPVAAQAPFPTRPIRLVVPAPAGDGSDALA
                     RSIAQELAAGLGQQVMIDNRPGAGGSIASDTVAKAAPDGHTLILGNGSSHGVTPSLYP
                     RLPYDALKDFAPITLVATAPNVLVVSASLPVHTVQDFLAYARARPGQLHIASGGNGSL
                     SHLSAELFKRMADVDLVHVPYKGAAPGLTDVAAGQVAAMVINIPSAQALLKAGRLQAL
                     ATTGRQRAAALPEVPTLDEAGLKGYETTSWFALLAPARTPTAVIERLQQEIARALATP
                     AVRERLAAQGLTPVGSTPAQFDAFMRAEIAKYARVIKDAGVKID"
     gene            complement(5049..5948)
                     /locus_tag="PKN51_11620"
     CDS             complement(5049..5948)
                     /locus_tag="PKN51_11620"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNT85788.1"
                     /translation="MSTAALHTSQLRSLPLLARGKVRDNYAVGEDRILMVASDRISAF
                     DVIMDQPIPGKGELLTRMALWWFEQLKDIVPNHLTGDAPESVVAPDEVAQVRGRSMLV
                     QRLQPIPVEAVVRGYLAGSGWKEYQQSQSVCGVPLPAGLHNASRLPQPIYTPAAKAAV
                     GEHDENITFERTEQMIGAELAARIRDISIQLYQRAAAIALARGIIIADTKFEFGLNAA
                     GELVLMDEVLTPDSSRYWPQDGWREGANPPSFDKQYLRDWLEQAQVNGRPWPKTAPPP
                     TLPAEVIEQTAAKYREALQRLLG"
     gene            complement(6038..7102)
                     /gene="fba"
                     /locus_tag="PKN51_11625"
     CDS             complement(6038..7102)
                     /gene="fba"
                     /locus_tag="PKN51_11625"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060402.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNT85789.1"
                     /translation="MPLVSMRELLDHAAEHNYGIPAFNVNNLEQVQAVMAAADEVGAP
                     VILQASAGARKYAGEHFIKYLIQAAAEAYPHVPMVMHQDHGTSPAVCAGALKLGFGSV
                     MMDGSLKEDGKTPADFDYNVRVTQEVVAMAHQIGATVEGELGCLGSLETGEAGEEDGI
                     GAAGKLDHSQLLTDPEEAAQFVKATQLDALAIAIGTSHGAYKFTRPPTGDVLAISRVK
                     EIHARIPNTHLVMHGSSSVPQELLAIINQYGGKMKQTWGVPVKEIQEAIKHGVRKINI
                     DTDIRMAMTGAVRKFQVENPDKFDMREWMKPARAAAQAICKQRYLEFGCEGQGAKVKG
                     LPLSVMAQKYAQGALAQQVR"
     gene            complement(7383..>7889)
                     /locus_tag="PKN51_11630"
     CDS             complement(7383..>7889)
                     /locus_tag="PKN51_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNT85790.1"
                     /translation="TDAVMLSAETAAGKYPLETVQEMAAICMAAEEHDPAELDADFSG
                     ATFQRIDQSIAMGALFTAYHLGAKAIVALTDSGSTALWMSRHRVRIPIYALTPKVGTQ
                     RKLALYRNVRPLLMDTSALRDEALAQAEAYLKQQGVMASGDVYAITCGEPMGAPGGTN
                     MLKICRAG"
BASE COUNT         1175 a         2682 c         2747 g         1285 t
ORIGIN      
        1 ttccaggttg aatcgccatc tagaatgcat ctttcccatt tctgcaacgc gagacgcgca
       61 tgagccgacc cacccgcaag ccccagatcc ccaacttctc ttccggcccc tgcgccaagc
      121 gccccggtta cgacgtggcc cggctcgacc tgtcggccct gggtcgctcg caccgcagca
      181 gcctgggcaa gcagctgctg ggcgccgcct gcagcgaaac cgcgcgcatc ctgggcctgc
      241 ccgccggcta ccgcgtgggc atcgtgccgg cgtcggacac cggcgccgtg gagatggtgc
      301 tgtggtcgat gctgggcgcg cgcggcgtcg acatgctggc ctgggaatcg ttcggcgccg
      361 gctgggtgac cgacgtggtc aagcagctca agctgaagga cgtgacgcgc cacgaggccg
      421 gctacggcga gctgcccgac ctgtccaagg tcaacttcgc caacgacgtg gtgttcacct
      481 ggaacggcac caccagcggc gtgcgcgtgc cggatggcga ctggatcgcc gacgaccgcg
      541 ccggtctgac catctgcgac gccaccagcg ccgtgttcgc gatggcgctg ccctggcaca
      601 agctggacgt ggtcacctac tcgtggcaga aggtgctggg cggcgagggc gcgcacggca
      661 tgctgatcct cagcccgcgc gcggtcgagc ggctggagag ctacagcccg ccctggccgc
      721 tgcccaagat cttccgcatg accagcggcg gcaagctgag cgagggcatc ttccagggcg
      781 acaccatcaa cacccccagc atgctgtgcg tggccgacta ccttgacgcc ctggcctggt
      841 gcgagcgcgt gggcggcgtg cccggcacca tcgcccgcag cacggccaac ctgaaggcga
      901 tcgaggactt cgtcgccgcc cacccctgga tctcgttcct ggcccgcagg cccgagacgc
      961 gctccaacac cagcgtgtgc ctgtcgctcg acctgccggc cgagcaggtc aaggccctgg
     1021 tcaagctgct ggaaaaggaa ggcgtggcct acgacatcgg ctcgtacaag gacgcgccgc
     1081 ccggcatccg catctggtgc ggcgccacgg tggaaaccgc cgacgtgcag gccctgctgc
     1141 cctggctggc ctgggcctac gaacaggtca aagcctgaca cgaacccgcg tcgaggcgca
     1201 cgcatggcgc atccgccggc tgcgccccgc cttccgaggg tcaaggttgt tctgatcgaa
     1261 tatgcccctg cggcgcaccc agtctgcgca aacagctatt aatatgtgag catcttatgt
     1321 tccggatcaa gacctacaac gccatctcgg taaaggggct ggagcgcttc gggcgcgagc
     1381 gctacgaagt cggccacgac atcgcccacc ccgacgccgt gctgctgcgc agccacaagc
     1441 tgcatggcga ggcgctgccg gcgtcgctgc tggcggtggc acgcgccggc gccggcgtca
     1501 acaacatccc ggtggccgac tacggacagc agggcatcgt ggtattcaac acgcccggcg
     1561 ccaacgccaa cgccgtcaag gaactggtgc tggccggcat gctgctggcc acgcgcggca
     1621 tcctgccggg catcgcctac gcgcaatcgc tggccggcat ggacgacgcc gccgccatgc
     1681 acgcactgct ggaaaaggaa aagtcccgct ttgccggcca cgagctcaag ggcaagacgc
     1741 tgggcctggt cggtctgggc gccatcggcg cgctggtggc cgacatggcg ctggcgctgg
     1801 gcatgaaggt gctgggctac gacccggtgc tgtcggtcga cgccgcctgg cgcctgtcca
     1861 acgcggtgca gcgcatggaa aacctgccgg cgctgctggc gcacgccgac tacgtgagcc
     1921 tgcacgtgcc ggccatggac gccacgcgcc acctgatcaa cgccgaggcg ctgcgccacc
     1981 tgcggcccag cgcggtgctg ctgaacttcg cgcgagaaac catcgtcgat ccggccgccg
     2041 tgctggcggc gctgcaggcc ggccggctgg ggcgctacgt gtgcgatttt cccgagccgg
     2101 gctttgccgc cgagcccagg gtgatcgccc tgccccacat cggcgccagc accgaggagt
     2161 cggaagaaaa ctgcgccgtc atggcggccg accagctgat cgacttcctg gagcacggcc
     2221 acatcgtcaa cagcgtcaac tacccggcgc tgcgcatgca ccgcgcgccc ggctcctgtc
     2281 gcatcgccat cgccaacgac aacgtggccg gcgtgctggg ccatgtgctg tcggcgctgg
     2341 ccgacgccgg cgtcaacgtg ctggacatga gcaaccgcag ccgcgaggcg ctggcctaca
     2401 acctgatcga cgtcgcatcg gcccccgagc cggcggtgat cgacgccatc cgccgcgtgc
     2461 cgcacgtgat ccgcgtgcgc acgctgtagc ccgggccgcg ccgcttcagg gctgttccac
     2521 gggcgggcgg tagccgtcgg tcagcgtccg caggaaggcc accacgtcgt cgatctcggc
     2581 gtcgttgagg gccggcgcgt cgcccggctg gcggtcgtag ggcgcttcgc tggtgttgac
     2641 gctggcgtgg tagtgggccg gcaggtcgtt gaacttgtcg acgctgccgt cggcgcggcg
     2701 cggataccac agctcgggct gggtgtcgcg cagcacgtag aagcgcaccg cgtccctgag
     2761 cgaggtgaac acgccgttgt ggaacagcgc ctggcgcagc gccacgttgc gcagcgaggg
     2821 caccttgaag gcgccgcaca ggtcgtcgcg cccggccaga tcggcgcgct gacacagccc
     2881 caggtcgtgg taggccggat cgtcgttggc ccgcagcgcc gggttgcgcg gcagacccag
     2941 gttgtcgtag gtgaaatcgg tgaacagcgg cagcgcaccc tggacgccgg gcgccgaggg
     3001 gtggcaggcg gcgcagttgc ccttggtcgg ctcgttgaac agcgccaggc cgcgcgcctc
     3061 ctgcgcgctc agctgggccg tgccgcgcag gtaggcgtcg tacttgctgg tgaaggcgtt
     3121 gaactccacg tcttccagct ggtagcgctg cagggcctcg gtcaggcgct ggtaggcgcc
     3181 ctcgacatcg ctgaagatcg cgtcgccata gacgcggcgg aactcggccg cgtacggcgc
     3241 ggcggccagc ttggtgatga cgctggcctt gtcgggattg gccatctcca gcgggttgag
     3301 gaagggcccg gcggcctgct cggccagggt ggcggcgcgc ccgtcccaga agaagccgcc
     3361 ggtaggcgtg ccctcctcgt cgaagaagaa cggggtgttg ctggccaggt agcggatgcc
     3421 aggcgtgttg cgcacgccct gcagcgtgcc gtccggcccg cccagctgcg ccggcagcgc
     3481 attgttggcg gcatggccgc gcgccgcgtc atggcaggtg gcgcaggact ggcggcccga
     3541 ggccgacagc gagacgtccg agaaaatctt ttctcccagc tgcgcctcgg ccgacagccg
     3601 agcgtcaccg gcgtcaccgc tgccgccgca gcccagcagc gcggcgctgg cacccagcgc
     3661 cagcagaaca cccaaccatg ggcgggcccg gaaaccacgg gacatgcgtc actcctccat
     3721 ccatcgcgcc agcgaccacg gcgctgcggg cgggattgta aacacgaatc gttcttgttt
     3781 ttatcctttc ttgacctttt ctgtcgggtg cggccggcga cgggtatgct gcgcctacgt
     3841 ccaaccacct gtcaccaact ccgatcatgc agcgcagatc atggctcgcc tgcctcgtgc
     3901 tgggctgggc gcccgttgcg gcgcaggcac cgtttccgac caggccgatc cggctggtgg
     3961 taccggcgcc ggccggcgat ggctccgacg cgctggcgcg ttcgatcgca caggagctgg
     4021 ctgctggtct gggccagcag gtgatgatcg acaaccgtcc gggcgccggc ggcagcatcg
     4081 cgtccgacac cgtggccaag gcggcgccgg acggccacac cctgatcctg ggcaacggca
     4141 gctcgcacgg cgtcacgccc agtctgtacc ccaggctgcc ctacgacgcg ctgaaggact
     4201 tcgcacccat caccctggtg gccacagcgc cgaacgtgct ggtggtcagc gccagcctgc
     4261 cggtacacac cgtgcaggat ttcctcgcct acgccagggc gcggcccggg cagttgcaca
     4321 ttgcctcggg cggcaacggc agcctgtcgc acctgtcggc cgaactgttc aaacgcatgg
     4381 cggatgtcga tctggtgcac gtgccctaca aaggcgccgc gcccggcctg accgacgtgg
     4441 ccgccggcca ggtggcggcg atggtcatca acatcccttc ggcccaggcc ctgctcaaag
     4501 cggggcggct gcaggcgctg gccaccaccg gccggcagcg cgcggcggcg ctgcccgagg
     4561 tccccacgct cgatgaggct ggcctgaagg gttacgagac cacgtcctgg tttgcactgc
     4621 tggcgccggc acgcactccg accgcggtga tcgagcggtt gcagcaggaa atcgcccgcg
     4681 cactggccac gcccgcggtg cgcgagcggc tggcagccca gggcctgacg ccggtgggca
     4741 gcacgccggc gcaattcgac gccttcatgc gcgctgaaat cgccaagtac gccagggtga
     4801 tcaaggacgc cggggtgaag atcgactgag cctaggaggc tgtcgggctt ggagcgccga
     4861 aagcgttctc gaagagcggc gcagccaaaa tcggccccag cgagaccagt ttttgccgga
     4921 tttgcagccc aatagtcgag ctattgggca aaaagccggt gaaaaatggg ccgctgcggt
     4981 cgatttgcag ccggcgattt ccaagtccga cagcctccta gggtcagagg ctgggacgcg
     5041 cgtgatcttc aacccagcag gcgctgcagc gcctcgcggt atttggcggc ggtctgctcg
     5101 atcacctcgg ccggcagcgt gggcggcggc gcggtcttgg gccaggggcg gccgttcacc
     5161 tgcgcctgct ccagccagtc gcgcaggtac tgcttgtcga agctgggcgg gttggcgcct
     5221 tcacgccagc cgtcctgcgg ccagtagcgc gacgaatcgg gcgtgagcac ctcgtccatc
     5281 agcaccagct cgccggccgc gttcaggccg aactcgaact tggtgtcggc aatgatgatg
     5341 cccctggcca gcgcgatggc ggcggcgcgc tggtacagct ggatgctgat gtcgcggatg
     5401 cgcgccgcca gttcggcgcc gatcatctgc tcggtgcgct cgaaagtgat gttttcgtcg
     5461 tgctcgccga ccgcggcctt ggccgccggg gtgtagatgg gctgcggcag gcggctggcg
     5521 ttgtgcaggc cggcgggcag gggcacgccg cacaccgact ggctttgctg gtattccttc
     5581 cagccgctgc cggccaggta gccgcgcacc accgcctcga ccgggatcgg ttgcaggcgc
     5641 tgcaccagca tgctgcgccc gcgcacctgc gccacttcat cgggcgccac cacgctctcg
     5701 ggcgcgtcgc cggtcaggtg gttgggcacg atgtctttca actgctcgaa ccaccacagc
     5761 gccatccgcg tcaacagctc gcccttgccg gggatgggct ggtccatgat gacgtcgaag
     5821 gcgctgatgc ggtcggaggc caccatcagg atgcggtctt cgccgacggc gtagttgtcg
     5881 cgcaccttgc cgcgcgccag caggggcagg ctgcgcaact ggctggtgtg cagggcggca
     5941 gtgctcatgg caggctccat tgcgatttca atgaaaagag ccactggcgc ttgtggatca
     6001 agcgcgagca gctcttattt ttgtagtggc tgaaggctca gcgcacctgc tgcgccagcg
     6061 cgccctgggc gtatttctgc gccatcacgg acagcggcag gcccttgacc ttggcaccct
     6121 ggccctcgca gccgaattcc agatagcgct gcttgcagat ggcctgggcg gcggcgcggg
     6181 cgggcttcat ccactcgcgc atgtcgaact tgtcggggtt ttcgacctgg aacttgcgca
     6241 ccgcgccggt catggccatg cggatgtcgg tgtcgatgtt gatcttgcgc acgccgtgct
     6301 tgatggcctc ttgaatctcc ttgaccggca cgccccaggt ctgcttcatc ttgccgccgt
     6361 actggttgat gatggccagc agttcctgcg gcacgctcga tgagccatgc atcaccagat
     6421 gggtgttggg gatgcgggcg tggatttcct tgacgcgcga gatcgccagc acgtcgcccg
     6481 tgggcgggcg ggtgaacttg taggcgccgt ggctggtgcc aatcgcaatg gccagggcgt
     6541 cgagctgcgt ggccttgacg aactgggcgg cttcttcggg gtcggtcagc agctggctgt
     6601 gatcgagctt gccggcggcg ccgatgccgt cttcctcacc ggcctcgccg gtttccaggc
     6661 tgcccaggca gccgagctcg ccctccacgg tggcgccgat ctggtgcgcc atggccacca
     6721 cttcctgcgt cacgcgcacg ttgtagtcga agtcggccgg ggtcttgccg tcttccttca
     6781 ggctgccgtc catcatcacc gaaccaaagc ccagtttgag cgcgccggcg cacacggccg
     6841 ggctggtgcc gtggtcctgg tgcatcacca tgggcacgtg cggataggct tcggcggcgg
     6901 cctggatcag gtatttgatg aagtgctcgc cagcgtattt gcgcgcgccg gcgctggcct
     6961 gcaggatgac gggggcgccg acctcatcgg cggcggccat cacggcctgc acctgctcca
     7021 ggttgttgac gttgaaggcg gggatgccgt agttgtgctc ggccgcgtgg tcgagcagtt
     7081 cgcgcatgga aacgaggggc atggtcaggg ttctccgaag gcagggcggt gtggcggcgg
     7141 ccgacgcccg ggtgtgcgac gagtttacct gctgcctctg accctagaaa cggcagttga
     7201 ccgcgtgtca acttcgggca gaccgcatcg atgcggggac cggcggcttc gagggccttc
     7261 acctcctggg tgcgcacgcc acgcacgcac ccggaccgcg ctcggcgcgc acgcgcgggg
     7321 cgtgcaaggc cgatggaccc caagggggcg cgaccggtct catgggcccg ccgctgcgcc
     7381 gatcagccgg cgcggcagat cttgagcatg ttggtgccgc cgggggcgcc catgggttca
     7441 ccgcaggtga tggcgtagac gtcgccgctg gccatcacgc cctgctgctt gaggtaggcc
     7501 tcggcctgcg ccagcgcctc gtcgcgcagg gcgctggtgt ccatcagcag cgggcgcacg
     7561 ttgcggtaca gcgccagctt gcgctgggtg ccgaccttgg gcgtgagcgc gtagatgggg
     7621 atgcgcacgc ggtggcggct catccacagc gcggtggagc cggaatcggt cagcgccacg
     7681 atggccttgg cgcccaggtg gtaggcggtg aacagcgccc ccatggcgat cgactggtcg
     7741 atgcgctgga aggtggcgcc actgaagtcg gcgtccagtt cggccgggtc gtgctcctcg
     7801 gccgccatgc agatggcggc catctcctgc accgtctcca gcgggtactt gccggccgcc
     7861 gtctcggcgc tcagcatcac ggcgtcggt
//
LOCUS       DAOQTZ010000116         7802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_10297_length_7802_cov_14.253001, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000116 DAOQTZ010000000
VERSION     DAOQTZ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10297_length_7802_cov_14.253001"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2001
                     /locus_tag="PKN51_11635"
     CDS             <1..2001
                     /locus_tag="PKN51_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNT85791.1"
                     /translation="FCGIHFFNPPRYMPLVELIATPATEARVIDQLEAFVTSDLGKGV
                     VRAKDTPNFIANRIGIAGMLSTMKEAENFGLSFDVVDDLTGKKLGRASSGTFRTADVV
                     GLDTMAHVIKTLQDNLSVETDPFYPSFETPAVLKKLIELGNLGQKTKAGFYKKAGRDI
                     LRFELESEDYVPGGQKADEVYARMLKKPAAERLKLLRNAEGAPGQFLWAILRNSFHYA
                     AIHLESIADCARDVDQAMRWGFGMKQGPFELWQEAGWLEVAKMVQEDIDAGRALCSAP
                     LPKWVFEGPVAEAGGVHTAAGSWSPSQQKFIARRVLPVYERQLFPEKLLGEDSLPDWQ
                     TAGTTIAESKALRTWTLDEDGSPFGGRVLIASIKNKMHAISPEVMEGLLEAVELAERE
                     YQAMVIWSGDAPFSVGADLEATMPAFVVGGADAIDSIEAELQNLMLRIRYAQVPVVAA
                     IHGMALGGGCELAVHCARRVAHMESYIGLVEVGVGLVPGAGGLTYIARRAAEAATGST
                     GKDLLPFLTEGFTAAAMAKVGTSAIESRKLGYLRDSDIIVPHRDELLFVAINEARAMA
                     AGGWRAPLPRPFPVAGRSGKATIMGSLVNMQGGGFISEFDQHIATLIANVVCGGDVDA
                     GTLVDEAYLMKLERQAFCQLIAHPKTHERILGMLNTGKPVRN"
     gene            2160..2555
                     /locus_tag="PKN51_11640"
     CDS             2160..2555
                     /locus_tag="PKN51_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNT85792.1"
                     /translation="MRVQNARALRASGTDAEQALWRHLRSRQLNGHKFRRQHPIGPYI
                     ADFACVEARLVVELDGGQHAQADALKHDERRTQFLLAQGWHVLRFWNHDMLAEPAGVL
                     QTIVQALAHPHPHPLPPAGEGVEQPWARA"
     gene            2593..3789
                     /locus_tag="PKN51_11645"
     CDS             2593..3789
                     /locus_tag="PKN51_11645"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNT85793.1"
                     /translation="MKQVQDAYIVAATRTPIGRSHKGYFRNYRPDDLLATALKAVMAQ
                     APGLDPAAVEDVIAGCAIPEAQQGLNVARIGAVLAGLPNSVGGITVNRFCASGLSAVA
                     MAADRIRVGEAEVMIAAGVESMSMVPMMGHSPSLSPSIFAPDSDVGIAYGMGLTAEKV
                     AQQWKVSREAQDAFAVESHRRAIAAQQAGEFADEITPVEVTDRGFDLASGQTTSRTRT
                     VNLDEGARPDTTLEGLAKLRTVFAARGSVTAGNSSQTSDGAGALILASEAAVKRFGLT
                     PLARFVSYASRGVPPSIMGIGPIEAIPAALRYAGLKQDDIDWYELNEAFAAQSLAVVN
                     TLGLDAAKVNPMGGAIALGHPLGATGAIRSATVVHALRRRQLKYGMVTMCVGMGQGAA
                     GIFERV"
     gene            3820..4767
                     /locus_tag="PKN51_11650"
     CDS             3820..4767
                     /locus_tag="PKN51_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT85794.1"
                     /translation="MPAARYQQAPTAIEMIASGAPVSALQLHTPRGGRVAAQLYQPPA
                     GRPVRWQVVIGPAMGVPQRFYAGFATWLAAQGARVLSFDYRGQADSLALLPHARLRQI
                     DATLHDWRADYEAATLHLHALAPDVPLLLVGHSLGAQLPGLFERSGHIDGLLAVAAGS
                     GYWRQNAPRLRSRALLMWYGMVPLLTPLFGYFPGRRLGAVGDLPAGVIRQWRRWCLHP
                     HYSGAEGPEARQRYAAVRFPIHALSVTDDEMMTLPGTQSLLALYAGAPHGIERVAPAD
                     HGLPRIGHLGWFHPRFEATLWPLTLRALGELAARTGPAT"
     gene            4834..5703
                     /locus_tag="PKN51_11655"
     CDS             4834..5703
                     /locus_tag="PKN51_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT85795.1"
                     /translation="MTRSALMTRHPFDDAVALAPLAASADERGPQAPGHFQGAPHAAY
                     GNMVGPFGGITAAQALAAVLRHPARLGEPVALTVNYAAALIEAPFRIEARPVRTNRST
                     QHWTVVLMQTDAQGVDGVMLTATCVTAVRRATWSAVEVPRPAAPAPQQVPRAVPPKRV
                     RWIERYDMRPLAGPMPLQWDGSEADSLTRLWVRDDPPRPLDFLSLTALADVFFPRVWR
                     RRARMTPAGTVSMSVYFHAGADELAATGEGYLLGQARAQSFFNGFFDQSAELWNEAGH
                     LLATTHQVVYFKE"
     gene            5700..6431
                     /locus_tag="PKN51_11660"
     CDS             5700..6431
                     /locus_tag="PKN51_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT85796.1"
                     /translation="MSMTRPVALVVGAGDATGGAIARRFAAGGYTACVTRRDAARLDE
                     LRHAIEAAGGICHAFGSDARKEDEVVALVDTIERDIGPIEVLVFNIGANVPCSVLEET
                     ARKYFKIWEMACFGGFLNGREVARRMVGRGRGTIIFTGATASVRGAAGFAAFAGAKHA
                     LRALAQSMARELGPQGIHVAHCIIDGAIDTAFIRERFPERYALRAQDGILRPDSIAEA
                     YWTLHRQPRDAWTHELDLRPYMETF"
     gene            6428..7018
                     /locus_tag="PKN51_11665"
     CDS             6428..7018
                     /locus_tag="PKN51_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HNT85797.1"
                     /translation="MMKHFEFWFDFGSPAAYLAWTQIPAIEAATGAQAIWRPMLLGGV
                     FQATGNQSPAAVAAKGRYIFTDFDRFARRYGVPLRRNPHFPIHTLLPMRGAVGLQRAQ
                     PERLADYCRAMFQAIWVDGRNLNEPAEVGAVLAAAGFDPQALLALANHPDTKQALKDA
                     TDEAVRRGVFGAPTFFVGEQIFWGQDRIDFLKEALQ"
     gene            7015..7779
                     /locus_tag="PKN51_11670"
     CDS             7015..7779
                     /locus_tag="PKN51_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNT85798.1"
                     /translation="MNEILSQSEAGVLTLTFHRVERKNSINVAMYDALATAIERAVDD
                     AATRVLLIQGHETVFSAGNDIEDFLRNPPAGKDSPVFRFLRGIATFPKPLLAAVCGPA
                     VGVGTTLLFHCDLVYAGDNAAFSVPFVNLGLCPEAASSLLLPQTFGYHRAAEALLLGE
                     PFMAEAALEVGLVNRVLPPTECNAFAQQQARKLAAKPLSSLITTKQLMKGAQQEAVLR
                     RMDEEGAHFRRMLGEPAAREAMTAFMEKRKPDFSAC"
BASE COUNT         1207 a         2762 c         2683 g         1150 t
ORIGIN      
        1 ttctgcggca tccacttctt caacccgccg cgctacatgc cgctggtgga gctgatcgcc
       61 acccccgcca ccgaggcgcg cgtgatcgac caactcgagg ccttcgtcac cagcgacctg
      121 ggcaagggcg tggtgcgcgc caaggacacg cccaacttca tcgccaaccg catcggcatt
      181 gccggcatgc tctcgaccat gaaggaggcc gagaacttcg gcctgtcgtt cgacgtggtc
      241 gacgacctca cgggcaagaa gcttggccgt gccagcagcg gcaccttccg caccgccgac
      301 gtggtgggcc tggacaccat ggcccatgtc atcaagacgc tgcaggacaa cctgagcgtc
      361 gagaccgacc cgttctaccc cagctttgaa accccggccg tgctcaagaa gctcatcgag
      421 ctgggcaacc tgggccagaa gaccaaggcc ggcttctaca agaaggcggg gcgcgacatc
      481 ctgcgcttcg agctcgagag cgaagactac gtgcccggcg gccagaaggc cgacgaggtg
      541 tacgcgcgca tgctgaaaaa gcccgcggcc gagcgtctca agctgctgcg caatgccgag
      601 ggtgcgccgg gccagttcct ctgggccatc ctgcgcaaca gcttccacta cgccgccatc
      661 cacctggaga gcattgccga ctgcgcgcgc gacgtggacc aggccatgcg ctggggcttt
      721 ggcatgaagc agggcccgtt cgagctctgg caggaggccg gctggctcga ggtcgccaag
      781 atggtgcagg aggacatcga cgccggccgc gcgttgtgca gcgcgccgct gcccaagtgg
      841 gtctttgaag gccccgtggc cgaggccggc ggcgtgcata cggcggccgg ctcgtggagc
      901 ccctcgcagc aaaaattcat agcacgtcgc gtgctccccg tctacgaaag acagcttttc
      961 cctgaaaaac tgctcggcga ggacagcctg cccgactggc agacggccgg cacgacgatt
     1021 gccgaatcca aggcgctgcg cacctggacg ctcgacgagg atggcagccc cttcggtggg
     1081 cgggtcctga tcgccagcat caagaacaag atgcacgcca tcagccccga ggtcatggaa
     1141 ggcctgctcg aggccgtgga gctggccgag cgggaatacc aggccatggt gatctggtcg
     1201 ggcgacgcgc cgttctcggt cggcgccgac ctggaagcga ccatgccggc ctttgtggtc
     1261 ggcggcgccg acgccatcga cagcatagag gccgagctgc agaacctgat gctgcgcata
     1321 cgctatgcgc aggtgccggt ggtggcggcc atccatggca tggcgctcgg cggcggctgc
     1381 gagctggcgg tgcactgcgc gcgccgcgtg gcccacatgg aaagctacat cggcctggtc
     1441 gaagtgggcg tgggcctggt gcccggtgcc ggcggcctga cctacatcgc ccgccgcgcg
     1501 gccgaagccg ccaccgggag caccggcaag gacctgctgc ccttcctgac cgaaggcttc
     1561 accgccgccg ccatggccaa ggtcggcacc agcgccatcg aatcgcgcaa gctcggctat
     1621 ctgcgcgact ccgacatcat cgtgccgcac agggacgaac tcctgttcgt cgccatcaac
     1681 gaggcccgcg ccatggccgc cggtggctgg cgcgcgccgc tgccgcgccc cttccccgtg
     1741 gccggcagga gtggcaaggc gaccatcatg ggttcgctcg tcaacatgca gggcggcggc
     1801 ttcatcagcg agttcgacca gcatatcgcc accctgatcg ccaacgtggt ctgcggcggc
     1861 gacgtggacg caggcaccct ggtcgatgag gcctacctga tgaagctcga gcgccaggcg
     1921 ttctgccagc tcatcgccca tcccaagacg catgagcgca tcctgggcat gctcaatacg
     1981 ggcaagccgg tgaggaactg aggctgcagg ccatggtgca caaccaccct caccccagcc
     2041 ctctcccgcc agcgggagag ggtgaaaaac cggcgcatgc cgacgccttc ccgacagtgg
     2101 gagagggcga aaaaccggcg cgcgctggct ccctctcccg cgtgcgggag agggcagggg
     2161 tgagggtgca aaacgcgcgc gcactgcgtg ccagtggcac ggatgccgag caagcgctgt
     2221 ggcgccacct gcgcagccga caactgaatg ggcacaagtt ccgtcgccag caccccatcg
     2281 gcccctacat cgcagacttt gcctgcgtag aggccaggct ggtggtggag ctcgatggcg
     2341 ggcagcatgc ccaggccgat gcgctcaagc atgacgagcg gcgcacgcaa tttctgctgg
     2401 cccagggctg gcatgtgctg cgcttttgga accacgacat gctggcagag cccgccgggg
     2461 tgctgcaaac cattgtgcag gcgttggccc accctcaccc ccaccctctc ccgccagcgg
     2521 gagagggagt tgaacagcca tgggcgcggg cctgagcctg ccgccttcga gaaaccgcaa
     2581 ggagccatct ccatgaaaca agtacaagac gcctacatcg tcgccgccac gcgcacgccc
     2641 atcgggcgct cgcacaaggg ctatttccgc aactaccggc ccgacgacct gctggccacc
     2701 gcgctcaagg ccgtgatggc gcaggcaccc gggctggacc cggccgcggt cgaggatgtg
     2761 attgccggct gcgccatccc cgaggcccag cagggcctga acgtggcgcg catcggcgcc
     2821 gtgctggccg gactgcccaa cagcgtgggc ggcatcacgg tgaaccgctt ctgcgcctcg
     2881 ggtctttcgg ccgtggccat ggcggccgac cgcatccgcg tgggcgaggc cgaggtgatg
     2941 atcgccgccg gcgtggagag catgagcatg gtgcccatga tgggccacag ccccagcctg
     3001 tcgcccagca tcttcgcgcc cgacagcgac gtcggcattg cctacggcat gggcctgacg
     3061 gccgagaagg tggcgcagca gtggaaggtc tcgcgcgagg cgcaggacgc ctttgccgtg
     3121 gagtcgcacc gccgcgccat tgccgcgcag caggccggcg agtttgccga cgagatcacc
     3181 cccgtggagg tgaccgaccg cggttttgac ctggccagcg gccagaccac cagccgcacg
     3241 cgcaccgtca acctcgatga gggtgcccgc cccgacacca cgctcgaggg cctggccaag
     3301 ctgcgcacgg tgtttgccgc gcgcggctcg gtgacggcgg gcaacagcag ccagaccagt
     3361 gacggcgcgg gcgccctcat cctggccagc gaggccgcgg tcaagcgctt tggcctgacg
     3421 ccgctggcgc gctttgtcag ctacgccagc cgcggcgtgc cgccgtccat catgggcatc
     3481 ggccccatag aggccattcc cgccgcgctg cgctacgccg gcctgaagca ggacgacatc
     3541 gactggtacg agctcaacga ggcctttgcc gcgcagtcgt tggccgtggt caacacgctg
     3601 gggctcgatg ccgccaaggt caaccccatg ggcggcgcga ttgccctggg ccacccgctg
     3661 ggcgcgaccg gcgccatccg ctcggccacc gtggtgcatg cgctgcgccg caggcagctc
     3721 aagtacggca tggtcaccat gtgcgtgggc atgggccagg gcgcggccgg catctttgag
     3781 cgggtgtgac gccggtgggc cgttggttcc agatgattca tgcctgcggc gcgctaccag
     3841 caagcgccga cagctattga aatgatagct tccggagcgc cggtgtcggc cctgcaactg
     3901 cacacgccgc ggggcggtcg ggtggcggcg cagctgtacc agccacccgc cggccgtccg
     3961 gtgcgctggc aggtggtgat cgggccagcg atgggcgtgc cgcagcgctt ctacgccggt
     4021 ttcgccacct ggctggcggc gcagggtgcg cgcgtgctca gcttcgacta ccgcgggcag
     4081 gccgattcgc tggcgctgct gccgcacgcg cggctgcgcc agatcgacgc cacgctgcac
     4141 gactggcgcg ccgactacga ggccgcgacg ctgcacctgc atgcgctggc gcccgacgtg
     4201 ccgctgctgc tggtcgggca cagcctgggc gcgcagctgc ccggcctgtt cgagcgcagc
     4261 gggcacatcg acggcctgct ggcggtggcg gccggcagcg gctactggcg ccagaacgcg
     4321 ccgcgcctgc gcagccgggc gctgctcatg tggtacggca tggtgccgct gctgacgccg
     4381 ctgttcggct actttccggg ccggcgcctg ggggcggtgg gcgatctgcc ggccggtgtg
     4441 atccgccagt ggcggcgctg gtgcctgcac ccgcactaca gcggcgccga agggcccgag
     4501 gcgcgccagc gctacgcggc ggtgcgcttt cccatccatg cgctgtcggt caccgacgac
     4561 gagatgatga ccctgcccgg cacgcaaagc ctgctggcgc tgtacgccgg tgcgccgcac
     4621 ggcatcgagc gcgtggcgcc ggccgaccac ggcctgccgc gcatcggcca tctgggctgg
     4681 tttcacccgc gcttcgaagc cacgctgtgg ccgctcaccc tgcgggcgct gggcgaactg
     4741 gcggcccgca ccggccctgc cacctgagcg acagcgcgcg cgccgctgcg tgctaaggtc
     4801 acgcgttccc tgtctgtcca cctgatcctg cacatgacac gttccgccct catgacccgt
     4861 cacccgttcg acgacgcggt ggcgctggcg ccgctggccg ccagcgccga cgagcgcggc
     4921 ccacaggcgc cgggccattt ccagggggcg ccgcacgccg cctacggcaa catggtgggg
     4981 ccgttcggtg gcatcacggc ggcgcaggcg ctggcggcgg tgctgcgcca tccggcgcgg
     5041 ctgggcgagc cggtggcgct gaccgtcaac tacgcggcgg cgctgatcga ggcgccgttt
     5101 cgcatcgaag cgcgtccggt gcgcaccaac cgctccaccc agcactggac ggtggtgctg
     5161 atgcagaccg acgcccaggg cgtcgatggc gtgatgctca ccgccacctg cgtcaccgcc
     5221 gtgcggcgtg ccacctggag cgccgtcgag gtgccgcggc cggcggcgcc ggcgccgcag
     5281 caggtgccgc gcgccgtgcc gcccaagcgc gtgcgctgga tcgaacgcta cgacatgcgg
     5341 ccgctggccg gccccatgcc gctgcagtgg gacggcagcg aggccgacag cctgacgcgc
     5401 ctgtgggtgc gcgacgaccc gccgcgcccg ctggactttc tgagcctgac ggcgctggcc
     5461 gacgtgttct tcccgcgcgt gtggcgtcgc cgcgcgcgca tgacgccggc cggcacggtg
     5521 tcgatgtcgg tctattttca cgccggggcc gacgaactgg ccgccaccgg cgagggctac
     5581 ctgctcggtc aggcgcgggc gcagtccttc ttcaacggtt tcttcgacca gagcgccgag
     5641 ctgtggaacg aagccggtca tctgctggcc accacgcacc aggtggtgta cttcaaggaa
     5701 tgagcatgac gcgccccgtg gccctggtgg tgggcgccgg cgacgccacc ggcggcgcca
     5761 tcgcgcggcg ctttgccgcc ggcggctata ccgcctgcgt gacgcggcgc gacgccgccc
     5821 ggctggacga gctgcgccac gccatcgaag cggcgggcgg catctgtcac gccttcggct
     5881 ccgacgcacg caaggaagac gaggtggtgg cgctggtcga caccatcgag cgcgacatcg
     5941 gcccgatcga ggtgctggtg ttcaacatcg gcgccaacgt gccctgcagc gtgctggagg
     6001 aaaccgcgcg caagtacttc aagatctggg aaatggcctg cttcggcggc tttctgaacg
     6061 ggcgcgaagt ggcccggcgc atggtggggc gcgggcgcgg caccatcatc ttcaccggcg
     6121 ccacggcgtc ggtgcgcggg gcagccggct ttgccgcctt tgccggcgcc aagcatgcgc
     6181 tgcgtgcgct ggcccagagc atggcgcgcg agctcgggcc gcagggcatc catgtggcgc
     6241 actgcatcat cgacggcgcc atcgacaccg ccttcatccg cgagcgcttc cccgaacgct
     6301 atgcgctgcg cgcgcaggac ggcatcctgc ggcccgacag catcgccgag gcctactgga
     6361 cgctgcaccg gcaaccgcgc gacgcctgga cgcacgagct ggaccttcgg ccctacatgg
     6421 agacattctg atgaagcact tcgaattctg gttcgacttc ggcagccccg cagcctacct
     6481 ggcctggacg cagatcccgg ccatcgaggc cgccaccggc gcgcaggcga tctggcggcc
     6541 gatgctgctg gggggcgtgt tccaggccac cggcaaccag tcgcccgccg ccgtggcggc
     6601 caaggggcgc tacatcttca ccgacttcga ccgctttgcc cgccgctacg gcgtgccgct
     6661 gcgccgcaac ccgcatttcc cgatccacac cctgctgccg atgcgcggcg ccgtgggcct
     6721 gcagcgcgcc cagcccgagc gtttggccga ctactgccgc gccatgttcc aggccatctg
     6781 ggtcgacggg cgcaacctga acgagccggc cgaggtcggc gcggtgctgg cggcggcggg
     6841 cttcgacccg caggcgctgc tggcgctggc caaccacccg gacaccaagc aggccctgaa
     6901 ggacgccacc gacgaggcgg tgcggcgcgg cgtgttcggc gcccccacct tcttcgtcgg
     6961 cgagcagatt ttctggggcc aggaccgcat cgattttctg aaggaagccc tgcaatgaac
     7021 gagatcctgt cgcaatccga agccggcgtg ctgacgctca ccttccatcg ggtggagcgc
     7081 aagaactcca tcaacgtcgc catgtacgac gcgctggcca cggccatcga gcgcgcggtg
     7141 gacgacgccg ccacgcgggt gttgctgatc cagggccacg agacggtgtt ttcggccggc
     7201 aacgacatcg aggactttct gcgcaacccg ccggccggca aggattcgcc ggtgttccgc
     7261 ttcctgcgtg gcatcgccac ctttcccaag ccgctgctgg cggccgtgtg cggcccggcg
     7321 gtgggtgtgg gcaccacgct gctgtttcac tgcgacctgg tctatgcggg cgacaacgcg
     7381 gcgttttcgg tgcccttcgt caacctgggc ctgtgccccg aagccgcatc cagcctgctg
     7441 ctgccgcaaa cgttcggtta ccaccgcgcg gccgaagcgc tgctgctggg cgagcccttc
     7501 atggccgaag cggcgctgga ggtcggcctg gtcaaccgcg tgctgccgcc caccgagtgc
     7561 aacgcctttg cccagcagca ggcgcgcaag ctggccgcca agccactgtc cagcctgatc
     7621 accaccaagc agctgatgaa gggcgcacag caggaggcgg tgctgcggcg catggacgaa
     7681 gagggcgcgc atttccgtcg catgctgggc gagcccgccg cccgcgaagc catgaccgcc
     7741 ttcatggaaa agcgcaagcc ggatttcagc gcatgctgaa atccgccgca tcgtcagatg
     7801 cg
//
LOCUS       DAOQTZ010000117         7789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_10319_length_7789_cov_15.063486, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000117 DAOQTZ010000000
VERSION     DAOQTZ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10319_length_7789_cov_15.063486"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..935
                     /gene="pyrF"
                     /locus_tag="PKN51_11675"
     CDS             111..935
                     /gene="pyrF"
                     /locus_tag="PKN51_11675"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNT85799.1"
                     /translation="MNFLDQLRQAQAQNQSLLCVGLDPEPARFPAGVARDAEGIYAFC
                     ARIVEATADLVCAFKPQIAYFAAHRAEPQLERLIAHMRALAPQVPVILDAKRGDIGST
                     AEQYAKEAFERYGADAVTLSPFMGFDSVAPYLKYEGKGAFLLCRTSNPGGDDLQNQRL
                     ADAPGQPLLYEHVARLAQGPWNLNGQLGLVVGATYPAEIERVRQLAPSLPLLIPGVGA
                     QGGDAVATVRAGLTAAGPIIVNSSRAILYASSGADFADAARTAARATRAQLQAARP"
     gene            1045..2019
                     /locus_tag="PKN51_11680"
     CDS             1045..2019
                     /locus_tag="PKN51_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT85800.1"
                     /translation="MSPRQVLPLSALCVTALLAAPAVLAQGAYPSKPITLVVGYPPGG
                     STDLTGRTLGDALSRQLGVPVVIENVGGAGGTIGAQKVASAAPDGYTLLVGANNEVAI
                     SKLVTRTVKYDLKDFTPIGLIASQPMVLVASTTTGVKNLGEFIAKAKAAPGKYSYGSS
                     GVGTALHLAGEMVKERAGVFMTHIPYRGVAPLTSDLVGGNLEYGVFVLSSGLPHIKSG
                     KVVALGTTEARRSPLTPDIPALGEHPQLKGIDIGTWFVLMGPARLPEAVTTRLKTALA
                     EALKAPEFRQKMEASGSTVAAPGGDVQRFLASEVAKYQKIVQFAKIEQ"
     gene            2016..3005
                     /locus_tag="PKN51_11685"
     CDS             2016..3005
                     /locus_tag="PKN51_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNT85801.1"
                     /translation="MSATLRFELPRPDIAAWRAGNTGVPGVWHFDSGQPGRALMITAL
                     IHGNELCGAWALKGLLEAGLRPVRGTLTLAFCNLAAFDRFDADRHDAARFVEQDLNRQ
                     WTPERLRAADSLERRRAAELAPFAERADWLLDLHSMHEPSAPLLLTGVQPRNLALARQ
                     LAAPEHIVIDAGHSDGVRLRDYGRFGLPDAQAGDARSLLIECGFHGDLASRAVAQDMC
                     RRLLQAAGTLEPAALDATLPGWRLPDAPRQWALTVDGAVVARSPRFAFTGDFTGLERF
                     AHAGTVIGHHDGPGQGEPVVTPYDDCVLVMPSVRQAREGVTVVRFARRAPLME"
     gene            3084..4010
                     /locus_tag="PKN51_11690"
     CDS             3084..4010
                     /locus_tag="PKN51_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNT85802.1"
                     /translation="MELRQLRYFVRVCEAGSMGKAALDLGMATSALSQQISRLEGELA
                     TRLLQRHATGVLPTDAGLAFLQQAQLTLRHAEDAVHAAQTARLSGRVSIGLASTTASV
                     LGVPLMHAMGQRYPAVRVHLVEALSGHLTTMLNARQLDLAILFRADGARRWSVVPLLD
                     EKLYVIGTPELPGMPAGPRVRLSQLRDLPLILPSPSHGLRALVDAAFARQRIAPCVHL
                     EVDGLSLLMDAVRAGLGATIQPGAAAARLAAGEVRRVLVADAQTGRRNLLASLSDDEL
                     SPAALAARVVLGDVARALVREGRWVGATLHKN"
     gene            4088..5497
                     /gene="tcuA"
                     /locus_tag="PKN51_11695"
     CDS             4088..5497
                     /gene="tcuA"
                     /locus_tag="PKN51_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HNT85803.1"
                     /translation="MVDVLVIGGGNAALCAALMAREAGASVLLLEAAPREWRGGNSGH
                     TRNLRCMHDAPQDVLVDAYPEEEYWQDLLKVTAGKTDEHLARLVIRHSSSCRDWMRRH
                     GVHFQPPLSGALHVARTNAFFMGGGKALVNAYYRSAAELGVRIRYQAPVDRLEIEHGR
                     FVAAHVQGERITARACVLAAGGFESNRDWLRQAWGRNERGEWPADNFLIRGTAYNQGV
                     LLRHMLDDHGADRIGDPTQAHMVAIDARAPLYDGGICTRIDCVSLGVVVNRDAERFYD
                     EGEDFWPKRYAIWGRLVAQQPGQIAWSIIDAKAIGRFMPPVFPGVVAATLPELARRLQ
                     LDEAAFVRTLDSYNAACVPGRFDHTALDDCRTDGLTPPKTHWARPIDTPPFYAYAVRP
                     GVTFTYLGLRTDDTAAVRFAERPSDNLFVAGEMMAGNVLGQGYTAGVGMSIGTAFGRI
                     AGVNAARAAINKGAFHGLA"
     gene            5484..6659
                     /gene="tcuB"
                     /locus_tag="PKN51_11700"
     CDS             5484..6659
                     /gene="tcuB"
                     /locus_tag="PKN51_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HNT85804.1"
                     /translation="MVSLEALAREARALANGEVVPMTGAETEVARQLTICNACRYCEG
                     FCAVFPAMTRRLEFGKADVHYLANLCHNCGACLHACQYAPPHEFMVNVPQAMATVRGQ
                     TYADYAWPPALGRLYKSNGLTLSLALALGLALFLLLAVLTKGSIAEVGVGGDFYAIFP
                     HNLLVWLFAPVFLFAVLALGMGVRGLLRQVTPATGGAAAGGAAAAEAAHDALRLKYLD
                     GGHGDGCHNVDDAHTLSRRRLHHFTFYGFLLCFAATSVGTLYHYLLGWPAPYELPSLP
                     KLLGAAGGVSLVIGTVGLWRLNRTRHPLHGDPAQKPMDLGFIALLFLVAASGLLLWWA
                     RGTVAMALLLCLHLGAVMALFATLPYGKFAHGVFRSAALLRYAVEKRQPNPIGLGSE"
     gene            6700..7677
                     /locus_tag="PKN51_11705"
     CDS             6700..7677
                     /locus_tag="PKN51_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT85805.1"
                     /translation="MNKRQLIRSAGALTCLVLTGGAASAQDFPPRKPVTLVVGFAAGG
                     AADGAARIIAKKLGENIGQTVVVDNKGGAGGNIAHQFTAKGPADGSLLLLGSIGPLTI
                     APHLMKVGYEPFKDLAPVSGGVNFPNVLVVHKGVGVKTLAEFVALARKPGARVEFAST
                     GAGSASHLAGEMFNQQAQIDMVHVPYKGGAPALQDLLGERISAYFAAPPTAMPHVEAG
                     KLVPLATTGLKRPSYLPNIPTVAESGYPGFEALNWYAFVAPGKTPAPILDRWNTEIVK
                     VLNDAEVKKALEAHGLTPQPTTRAELTDFMKKEDVKWSAIVKQRHIQAN"
BASE COUNT         1132 a         2768 c         2679 g         1210 t
ORIGIN      
        1 gtctcgcgtt gcagaaatgg gaaagatgca ttctagatgg cgattcaacc tggaatcggc
       61 acctgaccgc tggtcatcct cgggcgggcc gcctcctaga atggctgacc atgaactttc
      121 tcgatcagtt gcgccaggcc caggctcaga accagtccct gctgtgcgtc gggctggacc
      181 cggagccggc gcgctttccg gccggtgtgg cgcgcgacgc cgagggcatc tacgccttct
      241 gcgcccgcat cgtcgaggcc accgccgatc tggtgtgcgc cttcaagccg cagatcgcct
      301 actttgccgc gcaccgcgcc gagccgcagc tcgaacgcct gatcgcccac atgcgggcgc
      361 tggcgccgca ggtgccggtg atcctggacg ccaagcgcgg cgacatcggc agcacggccg
      421 agcaatacgc caaagaggcc ttcgaacgct acggcgccga cgccgtgacc ctgtcgccct
      481 tcatgggttt tgattcggtc gcgccttatc tgaagtacga aggcaagggc gccttcctgc
      541 tctgccgcac ctccaacccc ggtggcgacg acctgcaaaa ccagcgcctg gccgacgcgc
      601 ccggccagcc gctgctgtac gagcatgtgg cgcgcctggc ccaggggccg tggaacctga
      661 acggccagct cggcctggtg gtgggcgcca cctacccggc cgagatcgag cgcgtgcgcc
      721 agctggcgcc cagcctgccg ctgctcatcc ccggcgtggg cgcgcagggt ggcgacgccg
      781 tggccaccgt gcgcgccggg ctgacggcgg ccggccccat catcgtcaac agctcgcgcg
      841 ccatcctgta cgccagcagc ggcgccgact ttgccgacgc tgcccgcacc gccgcccggg
      901 ccacccgcgc gcagctgcag gcggcccggc cctgagggct gtcgtgcgct atattaataa
      961 tagcttttgg cgcacgtctg acaagcgcca aagccgtttt tgatccttgt tcaccgccca
     1021 ccctccacgc caagaaagct gcccatgagc ccacgccaag tcctgcccct gagcgccctg
     1081 tgtgtcaccg ccctgctggc cgcgccggcc gtgctggcgc agggcgccta ccccagcaag
     1141 ccgatcaccc tggtggtcgg ctacccgcca ggcggcagca ccgacctgac cggccgcacg
     1201 ctgggcgatg cgctgtcgcg ccagctcggc gtgccggtgg tgatcgagaa cgtgggcggc
     1261 gccggcggca ccatcggcgc gcagaaggtg gccagcgccg cgccggacgg ctacaccctg
     1321 ctggtgggcg ccaacaacga ggtggccatc agcaagctgg tgacccggac ggtcaagtac
     1381 gacctcaagg acttcacccc catcggcctg atcgcctcgc agcccatggt gctggtggct
     1441 tccaccacca ccggcgtgaa gaacctgggc gagttcatcg ccaaggccaa ggccgcgccc
     1501 ggcaagtaca gctacggcag ctcgggcgtc ggcacggcgc tgcacctggc cggcgagatg
     1561 gtcaaggagc gcgccggcgt gttcatgacc cacatcccct accgcggcgt ggcgccgctg
     1621 accagcgacc tggtgggcgg caacctggag tacggcgtgt tcgtgctctc cagcggcctg
     1681 ccgcacatca agagcggcaa ggtggtggcc ctgggcacca ccgaagccag gcgctcgccg
     1741 ctcacgcccg acatcccggc gctgggcgag cacccgcagc tcaagggcat cgacatcggc
     1801 acctggttcg tgctgatggg cccggccagg ctgcccgagg ccgtcaccac gcgtctgaag
     1861 accgcgttgg ccgaggcgct caaggcgccc gaattccgcc agaagatgga ggcctccggc
     1921 tccaccgtgg cggcgcccgg cggcgacgtg cagcggttcc tggccagcga ggtggccaag
     1981 taccagaaga tcgtgcagtt cgccaagatc gagcaatgag cgccaccttg cgcttcgagc
     2041 tgccgcgacc cgacatcgcc gcctggcgcg ccggcaacac cggggtgccc ggggtgtggc
     2101 acttcgacag cggccagccc gggcgcgcgc tgatgatcac cgccctgatc cacggcaacg
     2161 agctgtgcgg cgcctgggcg ctcaagggcc tgctggaggc cggcctgcgg ccggtgcgcg
     2221 gcacgctcac gctggccttc tgcaacctgg cggcgttcga ccgcttcgac gccgaccggc
     2281 acgacgccgc ccgcttcgtc gagcaggacc tgaaccgcca gtggacgccc gagcgcctgc
     2341 gcgccgccga ctccctggag cggcgccgcg ccgccgaact ggccccgttt gccgagcgcg
     2401 ccgactggct gctggatctg cattccatgc acgagcccag cgccccgctg ctgctcaccg
     2461 gcgtgcagcc gcgcaatctg gcgctggcac ggcagctggc ggcgcccgag cacatcgtca
     2521 tcgacgccgg ccacagcgac ggcgtgcgcc tgcgcgacta cgggcgcttc ggcctgcccg
     2581 acgcgcaggc gggcgacgcg cgctcgctgc tgatcgaatg cggctttcat ggcgacctgg
     2641 ccagccgcgc cgtggcgcag gacatgtgcc ggcgcctgct gcaggccgcc ggcacgctgg
     2701 agccggccgc gctggacgcc accctgcccg gctggcgtct gcccgacgcg ccgcgccagt
     2761 gggcgctgac ggtggacggt gcggtggtgg cgcgcagccc gcgctttgcc ttcaccggcg
     2821 acttcaccgg gctggagcgg tttgcccacg ccggcaccgt gatcggccac cacgacggcc
     2881 ccggccaggg cgagcccgtg gtcaccccct acgacgactg cgtgctggtc atgccctcgg
     2941 tgcgccaggc gcgcgaaggg gtgacggtgg tgcgctttgc gcgccgtgcc ccactcatgg
     3001 agtgatggct gccgaatcca tgggttcgga agcttcaatt tagactgcgc cgacagctcc
     3061 tgaattcata gcttacagcc gccatggaat tgcgccagct gcgttacttc gtccgggttt
     3121 gcgaggccgg cagcatgggc aaggcggcgc tcgacctggg catggcgacg tcggcgctga
     3181 gccagcagat cagccggctc gagggcgagc tggccacccg gctgctgcaa cgccacgcca
     3241 ccggggtgct gcccaccgac gccggactgg cctttttgca gcaggcgcag ctcaccttgc
     3301 gccatgccga agatgcggtg cacgcggcac agacggcgcg cctgtcgggc cgggtcagca
     3361 tcggcctggc atcgaccacc gcgtcggtgc tcggcgtgcc gctgatgcat gccatgggcc
     3421 agcgctatcc ggccgtccgc gtgcatctgg tcgaagcgct gtcgggccac ctgacgacga
     3481 tgctcaacgc gcgccagctc gacctcgcca tcctgtttcg cgccgatggc gcgcggcgct
     3541 ggagcgtggt gccgctgctc gacgagaagc tctatgtgat cggcacgccc gagctgccgg
     3601 gcatgccggc gggcccgcgc gtgcggctca gccagctgcg cgatctgccg ctcatcctgc
     3661 cgtcgcccag ccatggcctg cgggcgctgg tcgacgcggc ctttgcgcgc cagcgcatcg
     3721 cgccgtgcgt gcacctggaa gtggatggct tgtcgctgct gatggatgcc gtgcgcgccg
     3781 gcctgggcgc caccatccag cccggcgctg ccgctgcccg cctggcggcc ggcgaggtgc
     3841 gcagggtgct ggtcgccgac gcccagaccg ggcggcgcaa cctgctggcc agcctgtccg
     3901 acgatgagct gtcgcccgct gcgctggccg cgcgcgtggt gctgggcgac gtggcgcgcg
     3961 ccctggtgcg cgaaggccgc tgggtcggcg ccacccttca caagaactga acacccgttg
     4021 cgcgcggcgg tcttgccgac gggatggggc gtgcctacag tgcgcaccat tcccccagga
     4081 ggcaagtttg gtcgacgtac tcgtcatcgg tggcggcaac gccgccttgt gtgccgcgct
     4141 gatggcgcgc gaggctggcg ccagcgtgct gctgctggag gccgcgccgc gcgagtggcg
     4201 cggcggtaac tccggccaca cgcgcaacct gcgctgcatg cacgatgcgc ctcaggatgt
     4261 gctggtggac gcctatcccg aagaggagta ctggcaggac ctgctgaagg tgaccgccgg
     4321 caagaccgac gagcacctgg cgcgcctggt gatccgccat tcctccagct gccgtgactg
     4381 gatgcgtcgt cacggcgtgc actttcagcc gccgctgtcg ggcgccctgc acgtggcgcg
     4441 caccaacgcc ttcttcatgg gcggcggcaa ggcgctggtc aacgcctact accgcagcgc
     4501 cgccgagctt ggcgtgcgga ttcgctatca ggcgccggtc gatcgcctgg agatcgagca
     4561 cgggcgcttc gtcgccgcgc atgtccaggg cgagcgcatc accgccaggg cctgcgtgct
     4621 ggccgctggc ggcttcgaat ccaaccgcga ctggctgcgc caggcctggg ggcgcaacga
     4681 gcggggcgaa tggccggcgg acaacttcct gattcgcggc acggcctaca accagggcgt
     4741 gctgctgcgg cacatgctgg acgaccacgg ggccgaccgc atcggtgatc ccacccaggc
     4801 gcatatggtg gccatcgacg cccgcgcgcc gctgtatgac ggtggcatct gcacgcgcat
     4861 cgactgcgtc agcctgggcg tggtggtcaa ccgcgacgcc gagcgcttct atgacgaggg
     4921 cgaggacttc tggcccaagc gctacgccat ctgggggcgc ctggtggcgc agcagccggg
     4981 gcagatcgcc tggtcgatca tcgacgccaa ggccatcggc cgcttcatgc cgccggtgtt
     5041 tccgggcgtg gtggcggcga ccctgcccga gctggcccgc aggctgcagc tcgatgaagc
     5101 ggcgttcgtg cggacgctgg acagctacaa cgcggcctgt gtgcctggcc gcttcgatca
     5161 cacggcgctg gacgattgcc gcaccgacgg cctgacccca cccaagacgc actgggcacg
     5221 cccgatcgac accccgccgt tctacgccta cgccgtacgg ccgggcgtga ccttcaccta
     5281 cctcggcctg cgcaccgacg acacggccgc cgtacgcttt gccgagcggc ccagcgacaa
     5341 cctgttcgtt gccggcgaga tgatggccgg caacgtgctg ggacaaggct acacggcggg
     5401 cgtcggcatg tcgattggta cggcgtttgg gcggattgcg ggcgtcaatg cggcgcgggc
     5461 agctatcaat aaaggagctt tccatggtct cgcttgaagc cctcgcgcgc gaggcgcgtg
     5521 ccctggccaa cggcgaggtg gtgccgatga cgggtgccga aaccgaggtg gcacgccagc
     5581 tcaccatctg caacgcctgt cgctactgcg aaggcttctg tgccgtgttt ccggccatga
     5641 cgcgccgcct cgagttcggc aaggccgacg ttcactacct cgccaacctg tgtcacaact
     5701 gcggtgcctg cctgcacgcg tgccagtacg cgccgccaca cgagttcatg gtcaatgtgc
     5761 cgcaggccat ggccacggtg cgtggccaga cctacgccga ctacgcctgg ccgccggcgc
     5821 tgggccgcct gtacaagtcc aatggcctca cgctgtcgct ggcgctggcg ctggggttgg
     5881 cgctgttcct gctgctggcc gtgctgacga aggggtcgat cgcggaagtc ggcgtgggcg
     5941 gcgacttcta cgccatcttc ccgcacaacc tgctggtgtg gttgtttgcg ccggtgttcc
     6001 tgttcgccgt gctggcgctg ggcatggggg tgcgtggcct tctgcggcag gtcacgcccg
     6061 ccaccggcgg cgcggcggcc ggcggtgcgg cggcggccga ggccgcgcac gatgcgctgc
     6121 gcctgaagta cctcgacggc ggtcacggtg acggctgcca caacgtcgac gatgcccata
     6181 cgctgagccg gcggcgcctg caccacttca ccttctacgg cttcctgctg tgctttgccg
     6241 ccaccagtgt gggcacgctc taccactatc tgctcggctg gccggcgccc tatgagctgc
     6301 cgagcctgcc caagctgctg ggcgcagccg gcggcgtcag cctggtgatc ggcaccgtcg
     6361 gcctgtggcg cctgaatcgg acgcgccacc cgctgcatgg cgacccggcg cagaagccga
     6421 tggacctggg cttcatcgcg ttgctgttcc tggtggctgc cagcggcctg ctgctgtggt
     6481 gggcgcgcgg cacggtcgcc atggcgctgc tgctgtgcct gcacctgggg gcggtgatgg
     6541 cgctgttcgc caccttgccc tacggcaagt tcgctcacgg cgtgttccgc agcgcggccc
     6601 tgctgcgcta tgcggtcgag aagcggcaac ccaatcccat tggcctcggc tccgagtgag
     6661 cgcgggcgct cgatatccac cgttcgaagg agatccacga tgaacaagcg acaactgatc
     6721 cgcagtgccg gtgcgctgac ctgcctggtc ctgaccgggg gcgcggccag cgcgcaggac
     6781 tttccaccca ggaagccggt gaccctggtg gtgggctttg ccgccggcgg tgcggccgat
     6841 ggggcggcac gcatcatcgc caagaagctg ggcgagaaca tcggccagac ggtggtggtc
     6901 gacaacaagg gcggcgccgg cggcaacatt gcccaccagt tcacggccaa gggcccggca
     6961 gacggcagcc tgctgctgct cggctcgatc ggcccgctca ccatcgcgcc gcacctgatg
     7021 aaggtcggct atgagccatt caaggatctg gcgccagtgt cgggcggtgt caacttcccc
     7081 aacgtgctgg tggtgcacaa gggcgtcggc gtcaagaccc tggccgaatt cgtcgcgctg
     7141 gcgcgcaagc ccggcgccag ggtcgagttc gcgtcgaccg gcgccggctc tgcctcgcac
     7201 ctggctggcg agatgttcaa ccagcaggcc cagatcgaca tggtgcacgt gccgtacaag
     7261 ggtggcgcgc cggcgttgca ggatctgctg ggcgagcgca tcagcgccta cttcgccgcg
     7321 ccacccacgg ccatgccgca tgtcgaagcc ggcaagctgg tgccgctcgc caccaccggc
     7381 ctcaagcgcc cgtcctatct gccgaacatc ccgacggtgg ccgaatccgg gtatcccggt
     7441 ttcgaggcgt tgaactggta tgccttcgtc gcgccgggca agaccccggc accgatcctc
     7501 gaccgctgga acaccgagat cgtcaaggtg ctgaacgacg ccgaggtgaa gaaggcgctg
     7561 gaggcccatg ggctgacacc gcaacccacc acgcgggccg agctgacgga cttcatgaag
     7621 aaggaggacg tcaagtggag cgccatcgtc aagcagcgcc acatccaggc caactgagcc
     7681 tagaaactgc agttgaccgc ttgtgacctt cgggcaggcc gcatcgatgc ggggtcttgc
     7741 gacttcgatg gccttcacct cttgggtgag cacgctgcgc acccgcccg
//
LOCUS       DAOQTZ010000118         7740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_10401_length_7740_cov_13.925439, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000118 DAOQTZ010000000
VERSION     DAOQTZ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10401_length_7740_cov_13.925439"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..989)
                     /gene="rlmJ"
                     /locus_tag="PKN51_11710"
     CDS             complement(27..989)
                     /gene="rlmJ"
                     /locus_tag="PKN51_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNT85806.1"
                     /translation="MFSYRHAFHAGNHADVLKHLTLVATLRHLMRKDSPLTMIDTHAG
                     AGIYRLDAEAAQTSGEALAGIARLAPPNAGDSAPNSATARSRQAPDAIVSEYDALTDY
                     LALLAGFNRTGASGGPWRVYPGSPLLMHALMTEPARAAVHDRLRLFELHPTDAQALQA
                     HIAQLDAGRQVGMARQDGFAALKALLPPPASGSGSRRALVLIDPSYEIKSDYAKVAGA
                     VQDALRRFATGVYLVWYPIIPRPEAHTLPRRLRTLAQQAERAWLQATLAVGAAPGSNG
                     LSASGMFVINPPHTLAAALRSALPRVQQALARGPQASWSVDTGD"
     gene            1181..1609
                     /gene="rplM"
                     /locus_tag="PKN51_11715"
     CDS             1181..1609
                     /gene="rplM"
                     /locus_tag="PKN51_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNT85807.1"
                     /translation="MKTFSAKPADVTHEWFVIDATDKVLGRVASEVALRLRGKHKAIY
                     TPHVDTGDYIVVVNAAKLRVTGAKEQDKMYYRHTGYPGGIRETNFRDLQAKHPGRALE
                     KAVKGMLPKGPLGYAMIKKLKVYGGAEHPHSAQQPKVLEV"
     gene            1622..2014
                     /gene="rpsI"
                     /locus_tag="PKN51_11720"
     CDS             1622..2014
                     /gene="rpsI"
                     /locus_tag="PKN51_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNT85808.1"
                     /translation="MIGDWNNGTGRRKSSVARVFLKKGSGKITVNGKAIDEFFGRQTS
                     IMICKQPLVLTDNLETFDVQVNVRGGGESGQAGAVRHGITRALIDYDAGLKPQLSAAG
                     FVTRDAREVERKKVGLHSARRRKQFSKR"
     gene            2128..2583
                     /locus_tag="PKN51_11725"
     CDS             2128..2583
                     /locus_tag="PKN51_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNT85809.1"
                     /translation="MLGIKRQPSVRSVIGAGMRVQGPCAFPDGLQIDGAVLGDVVAEG
                     GQPSVLVIGEAGRVEGAVSADHVVIAGTVVGPVLARELLELQPTARVQGDVRYKTLEM
                     QQGAVIAGQLQPQLTPPPAAPEAPLGRASEPAEPTLDPEPGLPPNPDRP"
     gene            2674..3051
                     /gene="erpA"
                     /locus_tag="PKN51_11730"
     CDS             2674..3051
                     /gene="erpA"
                     /locus_tag="PKN51_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNT85810.1"
                     /translation="MSAVAQDLQTPTAMPDPIIFTDSAAAKVAELIAEEGNPDLKLRV
                     FVQGGGCSGFQYGFTFDEVANEDDTTMTKNGVSLLIDAMSYQYLVGAEIDYREDLQGA
                     QFVIKNPNAETTCGCGSSFSTAE"
     gene            complement(3202..4563)
                     /locus_tag="PKN51_11735"
     CDS             complement(3202..4563)
                     /locus_tag="PKN51_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT85811.1"
                     /translation="MKQTLISTGLALADFIHRHPKRLTAALAAVLLGGTGGAFAVASL
                     APAAPTAPLRLVSEAVQPSDLSAQAEALDQHAFTLYRSDQTRATDTPEALLKRLGLAD
                     PAAAAFLRKNETTRQALFGGRAVAGRSVTAEATDGAELQSLRTHWAESATDSHFKRLV
                     VERAGNGFSVRVETAPLVVNQRLASGTIRNALFAATDEAGLPDSVTRQLTEIFEANID
                     FHRGLRRGDRFAVVYETLEADGEPVRAGRILSAEVVNRGKTHQALWFKEPGASQGSYY
                     TPDGQSLRRAYLLSALKVSRITSGFGMRNHPVYGYSRSHAGVDYAAPTGTPVHTIGDG
                     TVEFAGVQSGYGKVVYIKHRNKKDTTVYAHLSQIGVKAGDPVSQGQVIGEVGQTGVAT
                     GPHLHLEFRVNNEPVDPTEMLAEQREHVPVSPAAKAAFARLKTDMRTQLTAAGQIALS
                     NFE"
     gene            4725..5957
                     /gene="tyrS"
                     /locus_tag="PKN51_11740"
     CDS             4725..5957
                     /gene="tyrS"
                     /locus_tag="PKN51_11740"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNT85812.1"
                     /translation="MSDNELTNAAVTEGVRAAMAISLRGCDELLPQQDWLKKLARAEA
                     TGQPLRIKLGLDPTAPDIHLGHTVVLNKMRQLQDLGHQVIFLIGDFTSLIGDPSGRNS
                     TRPPLTAEQIRVNAETYREQAFKVLDASKTELRYNSEWSDALGARGMIELAARYTVAR
                     MMERNDFAERFKAGQSISVHEFLYPLMQGYDSVALKSDIELGGTDQKFNLLMGRHLQQ
                     EYGQEPQCILTMPLLEGLDGVEKMSKSKNNYIGITEDANTMFAKVLSISDALMWRWYT
                     LLSFRSMAEIERLQAEVAAGRNPKEAKVLLAREITTRFHSAQAADAAEQDFAHRSRGG
                     VPDAIPEVQLSGAPLGIGVLLKQAGLAPSTSEANRLIDGGGVRVDASVVSDKALKLAA
                     GSYVVQVGKRRFARVTLA"
     gene            6049..7008
                     /locus_tag="PKN51_11745"
     CDS             6049..7008
                     /locus_tag="PKN51_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNT85813.1"
                     /translation="MGTLSAWLTPRHLMAASIGVALLTIALKAAAWWVTGSVGLLSDA
                     LESFVNLAAASFGFLMVTVAEQPPDAGHPFGHDKAEYFSSGFEGILIIGAALAIVWAA
                     LPRFWWPQPLEQLGWGMGLALASALLNGGLALVMLRASRRHRSIALEADARHLLTDVW
                     TTGGVIAGLLLAGATGWLWLDPLVAIGVALNIAREGVRLMRRSSQGLMDAAVEPDTQA
                     RIDAVLAQFAARHGGPAAVLFDHVLTRKAGQRRFVNLHMHLPPGWTLARADALRGEVE
                     RALLAAVPGLHASIQMLPDSVEPISTELGAAPAEAQRPSRDEP"
     gene            7084..7551
                     /gene="dtd"
                     /locus_tag="PKN51_11750"
     CDS             7084..7551
                     /gene="dtd"
                     /locus_tag="PKN51_11750"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNT85814.1"
                     /translation="MQALIQRVSQASVSVAGRVIGAIGPGLLVLLCAERGDDEAVADK
                     LLAKLLKLRIFSDAAGKMNLSVQDVAGGLLVVSQFTLAADTAGGNRPSFTGAAAPEDG
                     RRLYEHFVRQVRAAHPQVATGEFAADMQVALVNDGPVTIPLRVAPARPAVGHG"
BASE COUNT         1230 a         2571 c         2762 g         1177 t
ORIGIN      
        1 cgcgccatgg ccgcggcgcc agcggctcag tcgccggtgt ccacgctcca gctggcctgg
       61 gggccgcggg cgagggcctg ttggacccgg ggcagggccg accgaagggc cgcggccagg
      121 gtgtgcggcg gattgatgac gaacatcccg ctggccgaca gtccgttgct gccgggtgct
      181 gcgccgacgg cgagggtggc ctgcagccag gcgcgctcgg cctgctgcgc cagggtgcgc
      241 aggcggcgcg gcagcgtatg ggcctcgggg cgcgggatga tcgggtacca caccagatag
      301 acgccggtgg caaagcgccg cagggcgtcc tgaaccgcgc cggccacctt ggcgtagtcg
      361 ctcttgatct cgtagctggg gtcgatcagc accagggcac ggcgcgagcc gctgccgctg
      421 gccggcggcg gcagcagcgc cttcagcgcc gcaaagccgt cctgccgcgc catgccgacc
      481 tgccgccccg cgtcgagctg ggcgatgtgc gcctgcagcg cctgggcgtc ggtggggtgc
      541 agctcgaaca ggcgcaggcg gtcgtgcacc gcggcgcgcg ccggctccgt catcagggca
      601 tgcatcagca gcggcgagcc gggatacacc cgccaggggc cgccggaggc gcccgtgcgg
      661 ttgaagccgg ccagcagggc caggtagtcg gtcagcgcat cgtactcaga aacgatagcg
      721 tctggcgctt gtctggagcg cgctgtagcc gaattcggtg ctgaatcgcc ggcgttcggg
      781 ggcgccaggc gcgcgatgcc cgccagcgcc tcgcccgagg tctgcgccgc ttcggcgtcg
      841 agccggtaga tgccggcgcc cgcgtgcgtg tcgatcatgg tcagcgggct gtccttgcgc
      901 atcaggtggc gcagcgtggc caccagcgtc aggtgcttga gcacgtcggc gtggttgccg
      961 gcatgaaagg cgtggcgata gctgaacatg gcccgatggt agcggccctc ggcgcgtcgg
     1021 gcgccagcct tgcgcctttg ccccaaacgc cgctaaaatg gcgggctttg cagcgatctg
     1081 gccccgcggc aaaagcaagc atcccaccta agaggctccc gcagcaagag gagcgccgac
     1141 cgtggaaaag ggccaggcaa accctccaca aggaaaactc atgaaaacgt tcagcgcaaa
     1201 acccgctgac gtgacgcacg agtggtttgt gattgacgcc accgacaagg tgctcggacg
     1261 ggtcgccagc gaagtggccc tccgtctgcg cggcaagcac aaagccattt acacgcctca
     1321 cgtcgacacc ggcgactaca tcgtggtcgt caacgccgcc aagctccgcg tcactggcgc
     1381 caaggagcag gacaagatgt attaccgcca caccggctac ccgggcggca tccgcgaaac
     1441 caacttccgc gacctgcagg ccaagcaccc cggccgcgcg ctggaaaagg cggtcaaggg
     1501 catgctgccc aagggcccgc tgggttacgc catgatcaag aagctgaagg tctacggtgg
     1561 cgccgagcat ccgcacagcg cccagcagcc caaagtgctt gaagtctaag gagccgacga
     1621 gatgattggt gattggaaca atggcaccgg ccgtcgcaag tccagcgtcg cccgcgtgtt
     1681 tctgaaaaaa ggctccggca agatcacggt caacggcaag gccattgacg agttcttcgg
     1741 ccgtcagacc tcgatcatga tctgcaagca gccgctggtg ctgaccgaca acctggagac
     1801 cttcgacgtc caggtcaacg tgcgcggcgg cggtgaatcg ggccaggccg gtgccgtgcg
     1861 ccacggcatc acgcgcgccc tgatcgacta cgacgcgggc ctgaagccgc agctgtcggc
     1921 cgccggtttc gtgacgcgcg acgcgcgcga ggtcgagcgc aagaaggtcg gcctgcactc
     1981 cgctcgccgg cgcaagcagt tcagcaagcg ctgatcgggc ttggcgccct ggcgcaaagg
     2041 ccgcgccgcc ttccgggcgg tgcggctttt ttgtgggccg cgtggtaaaa agggcgtgca
     2101 agccatcgcg gtggcgggag agcagacatg ttgggcatca agcggcagcc atccgtgcgc
     2161 agcgtcatcg gcgcgggtat gcgggtgcag gggccttgcg catttccgga cggcctgcag
     2221 atcgacggtg cggtgctggg cgatgtggtg gccgagggcg gtcagcccag cgtgctggtg
     2281 atcggcgaag ccggccgcgt ggaaggcgcc gtcagcgccg accacgtggt gattgccggc
     2341 acggtggtcg ggccggtgct ggcgcgcgag ctgctggagc tgcagccgac ggcgcgcgtg
     2401 cagggcgatg tgcgctacaa gacactggag atgcagcagg gcgcggtgat tgccgggcag
     2461 ctgcagcccc agctcacacc gccgccggcg gcgcccgagg cacccctggg gcgcgccagc
     2521 gaaccggccg agccgacgct ggacccggaa ccaggcctgc cacccaaccc cgaccgcccc
     2581 tgagggacga tgcgccctgg cctgcctaat tccgactgat tcgatatact atttttctgg
     2641 cccgccggtg cgggcccgac gcaggagccc accatgagtg ccgttgccca agaccttcag
     2701 acccccacgg ccatgcccga tcccatcatc ttcaccgaca gcgcggccgc caaggtggcc
     2761 gagctgattg ccgaagaagg caaccccgat ctgaaactgc gcgtgttcgt gcagggcggc
     2821 ggttgttcgg gctttcagta cggcttcacg ttcgacgagg tcgccaacga ggacgacacg
     2881 accatgacca agaacggcgt gtcgctgctg atcgacgcca tgagttacca gtacctggtc
     2941 ggcgccgaga tcgactaccg ggaagacctg cagggggcgc agttcgtcat caagaacccg
     3001 aacgccgaga ccacctgcgg ctgcggctcg tcgttttcga cggcggagtg agccggccgc
     3061 cggcgcatcg gcagaccagc atcgaagaaa agggcgccat gggcgccctt ttctggtcat
     3121 aaccgatcaa aaactgcgcc gatcaagcgg caatagctca ctatttcata gcgttctgcg
     3181 gcgtgccgga ccgccggccg ctcactcgaa gttgctgagg gcgatctggc cggccgccgt
     3241 cagttgggtg cgcatgtcgg ttttaaggcg tgcgaaggcg gccttggcgg cgggcgagac
     3301 gggcacatgc tcgcgctgtt cggccagcat ctcggtcggg tccaccggct cgttattgac
     3361 gcggaattcc aggtgcaggt gcgggccggt ggcgacgccg gtctgaccga cttcgccgat
     3421 cacctgcccc tggctcaccg ggtcgcccgc cttgacgccg atctgcgaca ggtgggcgta
     3481 gaccgtggtg tccttcttgt tgcggtgctt gatgtagacc accttgccgt agccgctttg
     3541 cacgccggcg aattcgaccg tgccgtcacc gatggtgtgc accggggtgc cggtgggggc
     3601 ggcgtagtcg acgcccgcat ggctgcggct gtagccgtac accgggtggt tgcgcatgcc
     3661 gaagccgctg gtgatgcgcg acaccttgag cgccgacagc aggtaggcgc ggcgcaggct
     3721 ctggccgtcg ggggtgtagt agctgccctg gctggcgccc ggctccttga accacagcgc
     3781 ctggtgcgtc ttgccgcggt tgaccacctc ggcgctcagg atgcgcccgg cacggaccgg
     3841 ctcgccgtcg gcttccagcg tttcgtagac cacggcaaag cggtcgccgc ggcgcaggcc
     3901 gcggtggaag tcgatgttgg cttcgaagat ttcggtcagc tggcgcgtca cgctgtcggg
     3961 cagcccggcc tcatcggtgg cggcgaacag ggcgttgcgg atggtgccgc tggccaggcg
     4021 ctggttgacc accagcgggg cggtttcgac acgcacgctg aagccgttgc cggcacgctc
     4081 gaccaccagg cgcttgaaat ggctgtcggt ggcgctttct gcccaatggg tgcgcaggga
     4141 ctgcaattcg gcgccgtccg tggcttcggc cgtcacactg cggccggcca ccgcgcgccc
     4201 gccgaacaac gcctggcggg tggtttcgtt cttgcgcaga aaggccgcgg cggccggatc
     4261 ggccaggccc aggcgcttga gcagggcctc gggggtgtcg gtggcgcgcg tctggtcgct
     4321 gcggtagagg gtgaaggcgt gctggtccag cgcctcggcc tgggcgctca ggtcgctggg
     4381 ttgcaccgcc tccgagacca ggcgcagcgg cgcggtcggc gcggcgggcg ccagagaggc
     4441 gacggcaaag gcgcccccgg taccgcccag cagcacggcg gccagggcgg cggtgaggcg
     4501 tttggggtgg cgatgaatga aatcggcgag ggccaggccg gtggaaatca gggtttgctt
     4561 cacgcgcggc tgttccggga cagggtgttc aggacaggac agacacacac cgggcggtgt
     4621 gcgactgcca cgaagaaggc tcggcaaccg agcgcacccc taaaatcggg gcggtaacgg
     4681 gtcagtataa cgagcccggc cgacccctac aacctgaccg tacgatgtct gacaacgaac
     4741 ttacaaacgc cgccgtcacc gagggtgtgc gcgccgccat ggccatttcg ctgcgcggat
     4801 gcgacgaatt gctgccgcag caggactggc tgaagaagct ggcccgcgcc gaagccaccg
     4861 gccagccgct gcgcatcaag ctggggctgg acccgacggc gcccgacatc cacctgggtc
     4921 acaccgtggt gctgaacaag atgcgccagc tgcaggacct ggggcatcag gtgatctttc
     4981 tgatcggcga cttcaccagc ctgatcggcg acccgtccgg gcgcaacagc acgcgcccgc
     5041 cgctcacggc cgagcagatc cgcgtgaacg ccgaaaccta ccgcgagcag gccttcaagg
     5101 tgctggacgc cagcaagacc gagctgcgct acaacagcga atggtcggac gccctgggcg
     5161 cgcgcggcat gatcgagctg gccgcccggt acaccgtggc gcgcatgatg gagcgcaacg
     5221 actttgccga gcgcttcaag gccggccagt cgatcagcgt gcacgagttc ctctacccgc
     5281 tgatgcaggg ctacgactcg gtggccctga agagcgacat cgagctgggc ggcaccgacc
     5341 agaagttcaa cctgctgatg ggccgccacc tgcagcagga gtacgggcag gagccgcagt
     5401 gcatcctgac catgccgctg ctcgagggcc tggacggcgt cgagaagatg tccaagtcca
     5461 agaacaacta catcggcatc accgaagacg ccaacaccat gttcgccaag gtgctgtcga
     5521 tcagcgacgc gctgatgtgg cgctggtaca ccctgctgtc gttccggtcc atggccgaga
     5581 tcgagcggct ccaggccgag gtcgccgccg gccgcaaccc caaggaggcc aaggtgctgc
     5641 tggcgcgcga gatcaccacg cgctttcaca gcgcccaagc cgccgacgcc gccgagcagg
     5701 actttgccca ccgcagccgc ggcggcgtgc ccgacgcgat ccccgaggtg cagctcagtg
     5761 gcgcgccgct gggcatcggc gtgctgctca agcaggccgg gctggcgcct tccaccagcg
     5821 aggccaaccg cctgatcgac ggcggcggcg tgcgcgtcga cgccagcgtg gtcagcgaca
     5881 aggcgctcaa gctggccgcc ggcagctacg tggtgcaggt gggcaagcgc aggtttgccc
     5941 gcgtgacctt ggcgtgaact cctgttttga tagctgctgg cgcttgttgg tattgcgcca
     6001 gcgccatttt ttgcctgcaa ctcgcggccg gtgggcgagc gcctgagcat gggcaccctg
     6061 agcgcctggc tgacgccgcg ccacctgatg gcggcctcca tcggcgtggc cctgctgacc
     6121 atcgcgctga aggccgcggc gtggtgggtc accggctcgg tggggctgct gtcggacgcg
     6181 ctggaatcct tcgtcaacct ggctgccgcc agcttcggct tcctgatggt gacggtggcc
     6241 gagcagccac ccgatgcggg ccacccgttc ggtcacgaca aggcggagta cttttcttcc
     6301 ggcttcgagg gcatcctgat catcggcgcc gcgctggcca tcgtctgggc tgcgctgccg
     6361 cgcttctggt ggccgcagcc gctggagcag ctgggctggg gcatggggct ggcgctggcc
     6421 agcgcgctgc tcaatggcgg cctggcgctg gtgatgctgc gcgcctcgcg ccggcaccgc
     6481 tcgatcgcgc tggaagccga cgcgcgccac ctgctgaccg acgtctggac cactggcggc
     6541 gtcatcgccg gcctgctgct ggcgggcgcc accggctggc tgtggctgga tccgctggtg
     6601 gccatcgggg tggcgctgaa catcgcccgc gaaggggtgc gtctgatgcg gcgctcgtcg
     6661 cagggcctga tggacgccgc cgtcgagccc gacacgcagg cgcgcatcga cgccgtgctg
     6721 gcgcagttcg ccgcgcgcca cggcgggccg gccgcggtgc tgttcgacca cgtgctgacg
     6781 cgcaaggccg gccagcgccg cttcgtcaac ctgcacatgc acctgccgcc cggctggacc
     6841 ctggcgcgcg ccgacgccct gcgcggcgag gtcgagcggg cgctgctggc ggcggtgccg
     6901 gggctgcacg ccagcatcca gatgctgccc gacagcgtgg agccgatctc caccgaactg
     6961 ggcgcggccc cagccgaggc gcagcgccct tcacgagacg aaccatgacg cacgaccccg
     7021 ccgggcgccg atggccgcgg cgccatggtt tgtcgttcaa cgcaccacgg tagccaaccc
     7081 accatgcaag ccctgatcca gcgtgtgtcc caggccagcg tcagcgtggc ggggcgcgtc
     7141 atcggcgcca tcggaccagg cctgctggtg ctgctgtgcg ccgagcgtgg cgacgacgag
     7201 gccgtggccg acaaactgct ggccaagctg ctgaagctgc gcatcttcag cgacgccgcc
     7261 ggcaagatga acctgagcgt gcaggacgtg gccggcggcc tgctggtggt cagccagttc
     7321 acgctggcgg ccgacaccgc gggcggcaac cggcccagct tcaccggcgc cgccgcgccc
     7381 gaggatgggc gccggctgta cgaacacttc gtgcggcagg tgcgcgccgc acacccacag
     7441 gtggccacgg gcgaatttgc cgccgacatg caggtggcgc tggtgaacga cgggccggtg
     7501 acgatcccgc tgcgcgtggc gccggcgcgt ccggccgtcg gtcatggatg agggccgagg
     7561 gcatggcctg cccgtcgtgc cgttcggcat cagggggcgc atacagggcc aaaccgaacg
     7621 tctgtaacgc tgtgtgataa tgaccgcgtt ggcgctgcgt gtaccgacgt tttcggtggc
     7681 acggtgctcg acatgctcgc gggtgacggc ataaactggc gaggctcgtg tgattcgttt
//
LOCUS       DAOQTZ010000119         7557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_10744_length_7557_cov_12.820981, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000119 DAOQTZ010000000
VERSION     DAOQTZ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10744_length_7557_cov_12.820981"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..930
                     /locus_tag="PKN51_11755"
     CDS             136..930
                     /locus_tag="PKN51_11755"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNT85815.1"
                     /translation="MAALRHYLLALQFFTRIPVTGRLAAWVGYSPAMLNASAGHFPGV
                     GWVVGAAGAAVLWLAALRLPAGALGALVAAGLSTAATAWLTGAFHEDGLADTCDALGG
                     QVSRERALEIMKDSRIGSYGTLALVLALGLKLALLALLLQRGVAGAAAALLAAHVLSR
                     LAPLAVMRVLPYITEGPQAKSKPLADAVSGTSVVTAVLWSIPALALMGWSQGATETTV
                     AMALWALTLMAVLRWLRRRLQGFTGDTLGATQQLCELALYLGLALA"
     gene            927..1475
                     /locus_tag="PKN51_11760"
     CDS             927..1475
                     /locus_tag="PKN51_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNT85816.1"
                     /translation="MRLWLVRHAEVAAPPGLCYGASDLPADAAATRAAARALAERLPH
                     GLCLQVSPLQRCELLAQSLQALRPDLTLKFDARLREFDFGTWEGRPWGDIARAEFDAW
                     LADFALARPGGGECVAELMARVALAWDDWRASGREAAWVTHAGVIRAARLLAGGRRQV
                     AAATDWPAEGLAFGASLVLQAG"
     gene            complement(1609..2289)
                     /gene="tenA"
                     /locus_tag="PKN51_11765"
     CDS             complement(1609..2289)
                     /gene="tenA"
                     /locus_tag="PKN51_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HNT85817.1"
                     /translation="MNPASTTTFSQAAWQGNLALYHDTLALPFNQELATGTLSVERFH
                     HYIIQDAHYLVAYGRALAVAAAKADDADGLMQFTEAAKVAVLVERQMHGGFMQRFGVT
                     TEQFAATPLTPACHHYTQFLLATAWAEPYPVVLASLLPCFWIYAEVGRAIHASARQPN
                     PYQDWIDTYAGEEFHAAVRGVLTTIDRVADGVDARTLQAMHAAYTMSARLEWMFWDSA
                     HRLGRWPV"
     gene            2452..3036
                     /locus_tag="PKN51_11770"
     CDS             2452..3036
                     /locus_tag="PKN51_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNT85818.1"
                     /translation="MYQQCSLFADEAVTLVHDAEGSIVYHPACVPQASGALWLAALLA
                     QTPWRRLQRPMYERVVEVPRLVASVALRDPARPPCIDAALARVQAVAPAPYTHVGLNL
                     YRDGRDSVAPHGDRQAELEPGWPIAIVSLGAPRDMLIRALAGGASWRITLEPGSLLRM
                     SHASQRTHLHAVPKCAQALGPRISLAFRVRRSPP"
     gene            complement(2999..3838)
                     /locus_tag="PKN51_11775"
     CDS             complement(2999..3838)
                     /locus_tag="PKN51_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT85819.1"
                     /translation="MSTIVFSHGNSFPASTYRVMLDSLRARGFEVHAIEKYGHDPKYP
                     VTDNWPHLVRQLAQFATQRSKDDHGGAPWLVGHSLGGLLSLMCAARHPELARGVVMID
                     SPVLSGWRAGAVRVVKRTPLMRRLSPSSVARRRRKEWDDRQAVYEHFRAKKVFAAWDP
                     RVLRDYVDHGTLDADGSWRLAFDPEVESRIYETVPHNLGPLLRRHPLRCPVAFVGGIS
                     SVEMRQVGGLDATAEVAQGRVTMLEGSHLIPMEQPLAVAAAIEAHIRAMPTAATAAPG
                     TPG"
     gene            3968..4279
                     /locus_tag="PKN51_11780"
     CDS             3968..4279
                     /locus_tag="PKN51_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85820.1"
                     /translation="MSTPTPPSLPPELIDALRQRNLPQLLKVLLSQRPAASSPPAAPA
                     RPPAARPATGAGKRPPEAELPQVAAEGLSPGEVPRTGTTFWGWLMAALLAYLAYRLWG
                     G"
     gene            complement(4518..7088)
                     /gene="mutS"
                     /locus_tag="PKN51_11785"
     CDS             complement(4518..7088)
                     /gene="mutS"
                     /locus_tag="PKN51_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009931111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNT85821.1"
                     /translation="MMAQYLRLKADFPDTLLFYRMGDFYEMFYADAEKAAALLDITLT
                     TRGQSAGAPIPMAGVPFHSVDGYLARLIKLGESVAICEQVGDVATAKGPVERKVVRVV
                     TPGTLTDAELMSDKSESLLLAVHPGARGRIGLAWLAVTQGELHLAECASGELPGWIER
                     IGASELLLSQDAPPALQQALSQGPRAALTRRPDFQFDAPLGRRKLLEQLQAASLAGWD
                     AEELPHAHAACAALLSYAEHTQGRALTHVRALVVERAGTLIELPASTRRNLELVQTLR
                     GEDAPTLLSLLDTCLTGMGSRRLKSWLLAPARERAEASARLAAIGALRAGGLWQKLRA
                     HLKGSSDVERITARLALGQVRPRELVALRQTLQKTELVALVQQAPEPLLMQIAADLAP
                     PAEALDLLQAALLPEPAALIRDGGVIAPGLDQELDELRAISDNCDAFLLELEARERAR
                     TGIANLRVQFNKVHGFYIEVTQGQAAKVPDDYRRRQTLKNAERFITPELKAFEDKALS
                     AQERALAREKWLYEHLLNQLQPHVPQLTRLGRAIAALDALAALAERSLTLNWCAPEFT
                     AHPCIEIRAGRHPVVQARLQETSGQPFIANDTLLGPKARMQVITGPNMGGKSTYMRQV
                     ALIVLLASIGSYVPAQSCRLGPIDAIHTRIGAADDLANAQSTFMLEMTEAAQILHGAT
                     AQSLVLMDEIGRGTSTFDGLALASGIAAHLHDKVQAFTLFATHYFELTEFPATHHAAV
                     NVHVAAAETTGSGGHDIVFLHELQPGPASRSYGIQVARLAGVPAAVVHHARHALAQLE
                     QGASAAQVQVDLFAPPPPAAEPAPSPLDAALAALDPDQLSPREALDALYRLKKLARAG
                     "
BASE COUNT          996 a         2589 c         2840 g         1132 t
ORIGIN      
        1 agctggtgat cgtcaccaac ggcgactggg ccaaggccgg ctcggcgaac aacccgcagt
       61 acgccgcgcg cgaagccttc tatcagaccg tgcgccgcgc catcgacgac gaggcggccg
      121 ggcgctgagt tgcccatggc cgccctgcgc cactacctgc tcgccctgca gttcttcacc
      181 cgcatcccgg tcaccggccg gctggcggcg tgggtgggct acagcccggc catgctcaac
      241 gccagcgccg ggcactttcc gggcgtgggc tgggtggtgg gcgcggcggg cgcggcggtg
      301 ctgtggctgg cggcgctgcg gctgccggcc ggggcgctgg gcgcgctggt ggcggccggg
      361 ctgtccaccg ccgccacggc gtggctgacc ggtgcctttc acgaagacgg cctggccgat
      421 acctgcgacg cgctgggcgg gcaggtcagc cgagagcgcg cgctggagat catgaaggac
      481 tcgcgcatcg gcagctacgg cacgctggcg ctggtgctgg cgctgggtct caagctggcg
      541 ctgctggctt tgctgctgca acgcggtgtg gctggtgcgg cggccgccct gctggccgcc
      601 catgtgctgt cgcgcctggc gccgctggcg gtgatgcggg tgctgcccta catcaccgaa
      661 gggccgcagg ccaagtccaa gccactggcc gatgcggtgt ccggcaccag cgtcgttacc
      721 gctgttttgt ggtcgattcc ggccctggcg ctgatggggt ggtcgcaagg cgctaccgaa
      781 acaacagttg ccatggcgct gtgggcgctg acgctgatgg ccgtgctgcg ctggctgcgg
      841 cggcgcctgc aaggctttac cggcgatacc ctgggcgcca cccagcagtt gtgtgaactg
      901 gcgctgtacc tggggctggc gctggcatga ggctgtggct ggtgcgccat gccgaggtgg
      961 cggcgccgcc cggcctgtgc tatggcgcca gcgacctgcc ggccgatgcc gccgccaccc
     1021 gtgccgccgc gcgggcgctg gccgagcgcc tgccgcacgg gctgtgcctg caggtatcgc
     1081 cactacaaag atgtgagctg ctggcgcaat ccctgcaagc gctgcgaccc gatttgacct
     1141 tgaaattcga cgcccggctg cgcgagttcg acttcggcac ctgggaaggc cggccctggg
     1201 gcgacatcgc ccgagcggag ttcgacgcct ggctggccga ctttgccctg gcccgccccg
     1261 gcggcggcga gtgcgtggcc gagctgatgg cgcgcgtggc gctggcctgg gacgactggc
     1321 gcgccagcgg gcgcgaggcg gcgtgggtca cgcatgccgg cgtgatccgc gccgcgcggc
     1381 tgctggcggg cggccgccgc caggtggctg cggcgaccga ctggccggcc gaaggcctgg
     1441 cgtttggcgc gtcgctggtg ctgcaagccg gttgacgccg cctcgcgttc tggcagatga
     1501 cccatgacac cggttggcgc cggcatgacg aacgaccttt gggccattgg tcgtccaagc
     1561 gcagcgaggt cattggtcat gcgcgcgccg ccaccgcgca cggcggcgtc acaccggcca
     1621 gcggcccagc cggtgcgccg agtcccagaa catccattcg agccgcgcac tcatggtgta
     1681 ggccgcgtgc atggcctgca gcgtgcgcgc atccacaccg tcggccacgc ggtcgatggt
     1741 ggtcagcacg ccgcgcacgg cggcgtggaa ttcctcgccg gcgtaggtgt cgatccagtc
     1801 ctggtagggg ttgggctggc gcgcgctggc atggatggcc cggcccactt cggcgtagat
     1861 ccagaagcag ggcagcaggc tggccagcac caccgggtac ggctcggccc aggcggtggc
     1921 cagcaggaac tgcgtgtagt ggtggcaggc gggcgtgagc ggcgtggcgg cgaactgctc
     1981 ggtcgtcaca ccgaaacgct gcatgaagcc gccgtgcatc tggcgctcga ccagcaccgc
     2041 gaccttggcc gcctcggtga actgcatcag cccgtcggcg tcgtcggcct tggcggcggc
     2101 caccgccagc gcgcggccgt aggccaccag gtagtgcgcg tcctggatga tgtagtggtg
     2161 aaagcgctcg acgctcaggg tgccggtggc cagctcctgg ttgaagggca gggccagggt
     2221 gtcgtggtag agcgccaggt tgccctgcca ggcggcctgc gagaaggtgg tggtggaagc
     2281 cgggttcatg ggtgctgacg atcgccgggt ggccggcccg cgatcttatg ctgcggaatc
     2341 ttcaaatctg aaccgttcac cctgagccta gaaacggcag ctgacaagcg gtcaactgcc
     2401 gtttctagga taagtgcttg acagcgcgaa acactgcact agcatgactg tatgtatcaa
     2461 cagtgttcgc tgtttgccga cgaagccgtc accctggtgc acgatgccga aggcagcatc
     2521 gtctaccacc cggcgtgcgt gccgcaggcc agcggcgcgc tgtggctggc ggcgctgctg
     2581 gcgcagacgc cgtggcgccg cctgcagcgg ccgatgtacg agcgcgtggt cgaggtgccg
     2641 cggctggtcg ccagcgtggc gttgcgcgac ccggcgcgcc cgccctgcat cgacgccgcg
     2701 ctggcgcggg tgcaggccgt ggcgccggcg ccctacaccc atgtggggct gaacctgtac
     2761 cgcgacgggc gcgacagcgt ggcgccgcac ggcgaccgtc aggccgagct ggagccgggc
     2821 tggccgatcg ccatcgtgtc gctgggggcg ccgcgcgaca tgctgatccg cgcgctggcc
     2881 ggcggtgcca gctggcgcat cacgctggag ccgggcagcc tgctgcgcat gagccacgcc
     2941 agccagcgca cgcacctgca tgcggtgccc aagtgcgcgc aggcgctggg gccgcgcatc
     3001 agcctggcgt tccgggtgcg gcggtcgccg ccgtaggcat ggcgcggatg tgcgcctcga
     3061 tggcggcggc cacagccagc ggctgctcca tcgggatcag gtgcgagcct tccagcatgg
     3121 tcacgcggcc ctgcgccacc tcggcggtgg cgtccagccc gccgacctgg cgcatctcca
     3181 ccgacgaaat gccgccgacg aaggccaccg ggcaccgcag cggatggcgg cgcagcagcg
     3241 gccccaggtt gtggggcacg gtctcgtaga tgcgcgactc gacctcggga tcgaaggcca
     3301 ggcgccagct gccgtcggcg tccagcgtgc cgtggtccac gtagtcgcgc agcacccgcg
     3361 ggtcccaggc ggcaaacacc ttcttggcac ggaaatgctc atacacggcc tggcggtcgt
     3421 cccattcctt gcggcgccgg cgcgccacgc tgctgggcga gagccgccgc atgaggggcg
     3481 tgcgcttgac cacgcgcacc gccccggcgc gccagccgct cagcacgggt gaatcgatca
     3541 tcaccacgcc gcgcgccagc tcggggtggc gcgccgcgca catcaggctc agcaggccgc
     3601 ccagcgaatg cccgaccagc cagggcgcgc cgccgtggtc gtccttgctg cgctgggtgg
     3661 cgaactgcgc cagctggcgc accaggtgcg gccagttgtc ggtcaccggg tacttcggat
     3721 cgtggccgta cttctcgatc gcgtgcacct cgaagccgcg cgcccgcagg ctgtcgagca
     3781 tcacgcggta ggtgctggcc ggaaaactgt tgccgtggga aaagacgatg gtggacatgg
     3841 ggcggcattg tgccggggca gcgccgcggc gcgccgcccg ggggactggc caagccggcg
     3901 gccgggcttc acaatcggcg cgcgccccgg gccgtgccgg ccgctggcgc caccgacgaa
     3961 agccgccatg agcaccccga cacccccctc gctgccgccc gaactgatcg acgcgctccg
     4021 acagcgcaac ctgccgcaac tgctgaaggt cctgctcagc cagcggccgg ccgcctcgtc
     4081 accaccggcg gcgcccgcca ggccgccggc ggcgcggccc gccaccggtg ccggcaagcg
     4141 cccgccggaa gccgagctgc cgcaggtggc ggccgaaggc ctgtcgccgg gcgaagtgcc
     4201 gcgcaccggc accacgttct ggggctggct gatggcggcg ctgctggcct atctggccta
     4261 tcgcctctgg ggcggctgag ccgggaccgc gctgcagccg acccggccgc ccgctggcca
     4321 gggtgccggc tcgccctgcc ggccgccccg ggcagtcatc gaagccgggt gaggtcatgt
     4381 catcttggtt cggccttggg atgtcttgaa tgccattggg gctgagcttg tcgaagccct
     4441 gcgacaggct cagggtgaac ggttccatgg tcaagattcc gaagcaatcg ctcatgcgcg
     4501 cagcgcaggc gccgccttca gcccgcgcgc gccagcttct tcagccggta cagcgcgtcc
     4561 agcgcctcgc gcgggctgag ctggtcgggg tcgagtgcgg ccagcgccgc gtcgagcggc
     4621 gagggcgccg gctcggccgc gggcggcggc ggcgcgaaca ggtcgacctg gacctgcgcc
     4681 gcgctggcgc cctgttcgag ctgcgccagc gcgtggcgcg cgtggtggac cacggcggcc
     4741 ggcacgccgg ccaggcgcgc cacctggatg ccgtagctgc ggctggcggg ccccggctgc
     4801 agctcgtgca ggaacacgat gtcgtggccg cccgagcccg tggtttcagc cgcggcgacg
     4861 tgcacgttga cggccgcgtg gtgcgtggcc ggaaactcgg tcagctcgaa gtagtgggtg
     4921 gcaaacagcg tgaaggcctg caccttgtcg tgcaggtgcg cggcgatgcc gctggccagc
     4981 gccaggccgt cgaaggtgct ggtgccgcgg ccgatctcgt ccatcagcac cagcgactgg
     5041 gcggtggcgc cgtgcaggat ctgcgccgcc tcggtcatct ccagcatgaa ggtggactgg
     5101 gcgttggcca ggtcgtcggc ggcgccgatg cgggtgtgga tggcgtcgat cgggcccagg
     5161 cggcagcttt gggccggcac gtaggagccg atcgaggcca gcagcacgat cagcgccacc
     5221 tggcgcatgt aggtgctctt gccgcccatg ttggggccgg tgatgacctg catgcgcgcc
     5281 ttggggccca gcagcgtgtc gttggcgatg aagggttggc cgctggtctc ctgaaggcgt
     5341 gcctgcacca ccgggtggcg gccggcgcgg atttcgatgc aggggtgggc cgtgaactcg
     5401 ggcgcgcacc agttcagcgt cagcgagcgc tcggccagcg ccgccagcgc gtcgagcgcg
     5461 gcgatggcgc ggcccagccg ggtgagctgc ggcacgtgcg gctgcagctg gttcagcagg
     5521 tgctcgtaca gccatttttc gcgcgccaag gcgcgctcct gtgccgacag cgccttgtcc
     5581 tcgaaggcct tgagctcggg cgtgatgaag cgctcggcgt tcttcagcgt ctggcggcgc
     5641 cggtagtcgt cgggcacctt ggccgcctgg ccttgcgtga cctcgatgta aaagccatgc
     5701 accttgttga actgcacgcg caggttggcg atgccggtgc gcgcgcgctc gcgcgcttcc
     5761 agctccagca gaaaggcgtc gcagttgtcg ctgatggcgc gcagctcgtc gagctcctgg
     5821 tccagaccgg gggcgatcac gccgccgtcg cggatcagcg ccgccggctc gggcagcagc
     5881 gccgcctgca gcagatcgag cgcctcggcg ggcggtgcca gatccgcggc gatctgcatc
     5941 aaaagtggct ctggcgcttg ctggacaagc gccaccagct ctgttttttg tagtgtctgg
     6001 cgcagcgcca ccagctcgcg cgggcgcacc tgaccgagcg ccaggcgcgc cgtgatgcgc
     6061 tccacgtcgc tgctgccctt caggtgggcg cgcagcttct gccacaggcc gcctgcgcgc
     6121 agcgcgccga tggcggccag gcgcgcgctg gcctcggcgc gctcgcgcgc gggcgccagc
     6181 agccagcttt tcagccgccg gctgcccatg ccggtcaggc aggtgtcgag cagcgacagc
     6241 aacgtcggcg cgtcttcgcc gcgcagggtc tgcaccagct ccaggttgcg gcgcgtgctg
     6301 gcgggcagct cgatcagcgt gccggcgcgc tcgaccacca gggcgcgcac gtgcgtcagc
     6361 gcgcggccct gggtgtgctc ggcgtagctc agcagggcgg cgcaggcggc gtgcgcgtgc
     6421 ggcagctctt cggcgtccca gccggccagg ctggcggcct gcaactgttc cagcagcttg
     6481 cgccggccca gcggggcgtc gaactgaaag tccgggcggc gggtcagggc ggcgcgcggg
     6541 ccctgcgaca gggcctgctg cagcgccggc ggcgcgtcct gcgacagcag cagttcgctg
     6601 gcgccgatgc gctcgatcca gcccggcagc tcgccgctgg cgcattcggc caggtgcagt
     6661 tcgccctgcg tcaccgccag ccaggccagg ccgatgcgcc cgcgcgcgcc tgggtggacg
     6721 gccagcagca gggattcgct cttgtcgctc atcagctcgg cgtcggtcag cgtgccgggc
     6781 gtgaccacgc gcaccacctt gcgctcgacc ggccccttgg ccgtggccac gtcgcccacc
     6841 tgctcgcaga tcgccaccga ctcgcccagc ttgatcaggc gcgccagata gccgtcgacc
     6901 gaatgaaagg gcacgcccgc catcgggatg ggcgcgccgg ccgactgccc gcgcgtggtg
     6961 agggtgatgt ccagcagggc cgccgccttt tcggcgtcgg cgtagaacat ctcgtagaaa
     7021 tcacccatgc ggtagaacag cagggtgtcg ggaaagtcgg ctttcaggcg caggtactgc
     7081 gccatcatgg gggtgtgctg gccttcgggc ggttcacccg ggcgcgcggt gtggggttgg
     7141 ggcgcagcgg cctgcgcggt cggtcggggc atgggcagca tggtagcggc gcgctcgggc
     7201 gcgtcgccgg cacggcccct tgcgcgggtt ggcgtgtggt gcgtgccgac cggcttcacg
     7261 ccgggcggat gaagaacgac gaatgacctc gctgcgcttc gatggcggga ggggcgccca
     7321 ggctgccggc gcagccggtg tcaggggcgt gccaggggac gttcagcacg ggtccactga
     7381 aaccttgaaa tgggcggcga gggacacccg agacgggagg cgatgcaagg cgcaaagcgc
     7441 agccacagcc cgtgctgtgg cgagcatggg caacgccgca gcgtgcccgt atcgggcgga
     7501 cagcgccgaa ccgatttcag tgtttcagtg gacaaggttt agtggtcacc ggcgccc
//
LOCUS       DAOQTZ010000120         7408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_11018_length_7408_cov_13.444852, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000120 DAOQTZ010000000
VERSION     DAOQTZ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11018_length_7408_cov_13.444852"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /gene="pyk"
                     /locus_tag="PKN51_11790"
     CDS             complement(<1..982)
                     /gene="pyk"
                     /locus_tag="PKN51_11790"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNT85822.1"
                     /translation="MTYRATKIVATLGPASSDPALLEQMIRAGVNVVRLNFSHGKAED
                     HVERARLVRQASERAGREVAIMADLQGPKIRVGKFAEGKVLLAPGAPFVLDAARTEPG
                     DLQGVGLDYKQLPRDVRPGDTLLLNDGLIVLTVEAVRGEAVHTRVKLGGELSNNKGIN
                     KQGGGLTAPALTAKDMEDIKTAMRLRADYVAVSFPKNATDMEMARQLCNVAGAEWGHK
                     PQLIAKIERAEAIPRLAEILAASDGIMVARGDLAVEVGNAAVPALQKKMITMARAADK
                     LVITATQMMESMIANPVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPLE"
     gene            complement(1096..2292)
                     /locus_tag="PKN51_11795"
     CDS             complement(1096..2292)
                     /locus_tag="PKN51_11795"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNT85823.1"
                     /translation="MNVIRFSDLCAQGAVQGQRVFIRADLNVPQDDGGAITDDTRIRA
                     SVPAIRMALQAGAAVMVTSHLGRPTEGECKPADSLAPVARRLGELLGREVPLVQHWVD
                     GVTVAPGELVLLENCRVNKGEKKNDEALSRKMAALCDIYVNDAFGTAHRAEATTYGIA
                     QYAPVACAGPLLAAEIDALQQALAHPRRPLVAIVAGSKVSTKLTILQSLASKVDQLIV
                     GGGIANTFMLASGLPIGKSLAEADLVGEARAVIEAMRARGAEVPIPTDVVTATEFKAD
                     AVATVKPAQAVAADDLILDIGPDTAERLAAQLQAAGTIVWNGPVGVFEMAPFENGTRR
                     IARAIADSPAFSIAGGGDTLAAIAKYGIDKDVGYISTGGGAFLEVLEGKQLPAFEILE
                     KRAASA"
     gene            complement(2588..2935)
                     /locus_tag="PKN51_11800"
     CDS             complement(2588..2935)
                     /locus_tag="PKN51_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNT85824.1"
                     /translation="MHELDGWTLLAILGMTAISVITRGFFFISEREWTLPEWAQRGLQ
                     YAPIAALTAVIAPDILLTEGVVGAVLRDARTWGALAAAGFYFWRRRGSYVMPAAIAVG
                     MAVFLPLRVGLGW"
     gene            complement(2928..3617)
                     /locus_tag="PKN51_11805"
     CDS             complement(2928..3617)
                     /locus_tag="PKN51_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNT85825.1"
                     /translation="MAGVSIGLTAWAFMTGVAMVKSGMSITESVLMSLLVFAGSAQLA
                     AIPLIAADAPLWVVLAAAFCVNLRFVVFSLHLRPYFMHLPRFKRLYLGYFTGDVTYVL
                     FTRRFPRPPETEGQRRAQGSYLWGGNVTNWVFWQSLSMLGIFLGAALPSRWGLEFAGT
                     LALLAVACSMVTTRLRALSAITAAAAAVVLFGLPYRLNIVAAIAVAVAISLYAEPRPP
                     AAGDDAGARHA"
     gene            complement(3746..4873)
                     /locus_tag="PKN51_11810"
     CDS             complement(3746..4873)
                     /locus_tag="PKN51_11810"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNT85826.1"
                     /translation="MRVIFAGTPEFARVALQALLAAGFEVPLVLTQPDRPAGRGMKLQ
                     ASPVKQLALQHGIAVAQPRSLRLDGKYPEDAAAARAALRAARADAMVVAAYGLILPQW
                     VLDDMSTAGPPQGASAPSGGSATGAAVERGGRDPSTAGPPQGASAPSAGSATGQAFGC
                     LNIHASLLPRWRGAAPIHRAIEAGDAQTGVTIMQMDAGLDTGDMLLAQALPIGPRTTT
                     ASLHDELAALGGQLIVQALQQAAQGRLRPTPQPAEGVCYAHKIDKHEAAIDWHQPAAQ
                     IERRIRAFDPFPGASSQLGDTVVKLWSSEIDSCSRPSDARCGQILAVDEGGIAVACAD
                     AVLRLTELQRPGGKRLPAREFLRGFALAPGQCLGEGTGGAP"
     gene            complement(4943..5428)
                     /locus_tag="PKN51_11815"
     CDS             complement(4943..5428)
                     /locus_tag="PKN51_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85827.1"
                     /translation="MPAALGALLLGACAALLNPDRVAPGTPAAQVLQRLGPPTARYPA
                     TAQAGERWQYSQQPAGRQVFNLDLDAQGRVVRVEQALSEARFGQRIQPDRWTREDVLR
                     EYGRPAQVTAVHRFDGDIWVWRYLDGPTWRLLFIDIDRAGVVRGWSAGDENLPDPPEP
                     R"
     gene            complement(5485..6015)
                     /gene="def"
                     /locus_tag="PKN51_11820"
     CDS             complement(5485..6015)
                     /gene="def"
                     /locus_tag="PKN51_11820"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNT85828.1"
                     /translation="MAQLPILRYPDPRLHKVARPVQVVDARVQALIDDMFETMYAAKG
                     IGLAATQVDVHERLIVIDVSEERNQRLVLINPELLWASEQRVRGEEGCLSVPGIYDGV
                     ERAERVRVRALDAAGEAREIEAEGLLAVCIQHEMDHLLGKVFVEYLSPLKRGRIKTKL
                     LKAERQEKADQARVPA"
     gene            6159..>7408
                     /locus_tag="PKN51_11825"
     CDS             6159..>7408
                     /locus_tag="PKN51_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT85829.1"
                     /translation="MKHTFPGATRALIAPLGLLTAAVLSVPPAAAQSASRLPVTPAQR
                     ATAQQVAAQGVPLSALAPDAPDQYTVRRGDTLWRISSLFLKSPWRWPELWGMNLQDIR
                     NPHLIYPGQQLYLERLGDRAFLRARRGADGEVGDTVRVSPRNRIEMLGTGPLPTLEPH
                     LIEPFLTEPLVVDDDSFQRAPRIVALANQSRVLVAKGDRAYVRGPADAPLLKQAGDPT
                     EFRVFRNAKPLKDPVTGETLGYEGHYVGQATLVRGESEAEEDAGAAPAVRPTRWPANG
                     EVQSEPRRAAAPQPRALPVPATVDVISSKEEMRAGDRLLPEPPREYRNYVPRVPEVPV
                     DARVVAVPGSAVRYAGQNQVVVINKGLQDGIESGHVLALLSTGPKLVDKTDEARGLMK
                     LPDERNGIAMVFRPFERVSYVLVM"
BASE COUNT         1086 a         2655 c         2574 g         1093 t
ORIGIN      
        1 tctccagcgg gtacttgccg gccgccgtct cggcgctcag catcacggcg tcggtaccgt
       61 cgagcacggc gttggcgacg tcgctgacct cggcgcgcgt gggcaccgga ttggcgatca
      121 tgctctccat catctgggtg gcggtgatca ccagcttgtc ggccgcgcgc gccatggtga
      181 tcatcttctt ttgcagcgcc ggcacggcgg cgttgccgac ttccaccgcc aggtcgccgc
      241 gcgccaccat gatgccgtcg ctggcggcca ggatctcggc cagccgcggg atggcctcgg
      301 cgcgctcgat cttggcgatg agctgcggct tgtggcccca ttcggcgccg gccacgttgc
      361 acagctggcg cgccatttcc atgtcggtgg cgttcttggg aaagctcacg gccacgtagt
      421 cggcgcgcag gcgcatggcg gtcttgatgt cctccatgtc cttggccgtc agcgccggcg
      481 ccgtcaggcc accgccctgc ttgttgatgc ccttgttgtt ggacagctcg ccacccagct
      541 tgacgcgggt gtgcaccgct tcgccgcgca ccgcctccac cgtcagcacg atcaggccgt
      601 cgttgagcag cagggtgtcg cccggccgca cgtcgcgcgg caattgcttg tagtccagcc
      661 cgacgccctg caggtcgccc ggctcggtgc gcgcggcgtc cagcacgaac ggcgcgcccg
      721 gcgccagcag caccttgccc tcggcaaact tgccgacgcg gatcttcggc ccctgcaggt
      781 cggccatgat ggccacctcg cggccggcgc gctcgctggc ctggcgcacc aggcgggcgc
      841 gttccacatg gtcctcggcc ttgccgtggc tgaagttcag ccgcaccacg ttgacgccgg
      901 cacggatcat ctgctccagc agcgccgggt cgctggaggc agggccgagg gtggcgacga
      961 tcttggtggc gcggtaggtc atggctgggc tgggtgcagg cggaaatcgc tggccggatt
     1021 ctgccagcgc cgcatgacgg cgccgtgatc gggcgccaga ttactatgac ttttatagct
     1081 gttggcgctt gtccatcaag cgctggcggc ccttttttcc agaatttcga aggcgggcag
     1141 ctgcttgccc tccagcacct ccagaaaggc gccaccgccg gtggagatgt agcccacgtc
     1201 cttgtcgatg ccgtacttgg cgatcgccgc cagggtgtcg ccgccgccgg cgatgctgaa
     1261 ggcgggcgaa tcggcgatgg cgcgcgcgat gcggcgcgtg ccgttttcga acggcgccat
     1321 ctcgaacacg cccaccgggc cgttccagac gatggtgccg gccgcctgga gctgcgccgc
     1381 caggcgctcg gcggtgtcgg ggccgatgtc caggatcagg tcgtccgccg ccaccgcctg
     1441 cgccggcttg acggtggcca cggcgtcggc cttgaactcg gtggccgtca ccacgtcggt
     1501 ggggatcggt acctcggcgc cgcgcgcccg catggcctcg atcacggcgc gcgcctcacc
     1561 caccaggtcg gcctcggcca gcgacttgcc gatcggcagc ccgctggcca gcatgaaggt
     1621 gttggcgatg ccgccgccta cgatcaattg atcgactttg gacgccaaag actgcaagat
     1681 ggtgagtttt gtgctcactt tgctgccggc cacgatggcc accaggggcc gcctggggtg
     1741 cgccagggcc tgctgcaggg cgtcgatctc ggccgccagc agcgggccgg cgcaggccac
     1801 cggggcgtac tgggcgatgc cgtaggtggt ggcttcggcg cggtgcgcgg taccgaaggc
     1861 atcgttaacg taaatatcac aaagtgcggc catcttgcgc gaaagcgctt catcgttctt
     1921 tttctcgccc ttgttgacgc ggcagttctc gagcagcacg agctcgcccg gcgccaccgt
     1981 cacgccgtcc acccagtgct gcaccagggg cacctcgcgc cccagcagtt cgcccaggcg
     2041 gcgcgccacc ggcgccagcg agtcggcggg cttgcattcg ccctcggtcg ggcgacccag
     2101 gtgcgaggtg accatcaccg ccgcgccggc ttgcagcgcc atgcggatgg ccggcacgct
     2161 ggcgcggatg cgggtgtcgt cggtgatggc gccgccgtcg tcctgcggca cgttcaggtc
     2221 ggcgcggatg aagacgcgtt ggccctgcac ggcaccttgc gcgcacaggt cggagaagcg
     2281 gatgacgttc atgacaggca gatcgggtaa gtgaaaaaac acaggacccg ccggcggcgg
     2341 gagggcgatg cggcagcgcc gccattgtag gagcgcgcgc ccacaccacc ctgaccggtc
     2401 ccgggcttcg gattcgctac acgatcaata gctgctggcg cttgactgaa gggccacgtc
     2461 gcccggtttg cccatcatcc tcctgcttcg gaaacttgga aatccagacc ccaaaggcgc
     2521 catcaagggg aatcggatcg tccgtcccgc agccgccaga cccgggccga ccgggccgtg
     2581 cgccgcttca ccagcccagc ccgacccgca gcggcaggaa caccgccatg ccgaccgcga
     2641 tggcggccgg catcacgtaa ctgccgcgcc gacgccagaa gtagaaaccg gccgccgcca
     2701 gcgcgcccca ggtgcgggcg tcgcgcagca ccgcgccgac gacgccctcg gtcagcagga
     2761 tgtcgggcgc gatcaccgcg gtcagcgcgg cgatgggcgc gtactgcagg ccacgctgcg
     2821 cccattcggg cagcgtccat tcgcgctcgc tgatgaagaa gaagccgcgc gtgatgaccg
     2881 aaatggccgt catgcccagg atggccagca gcgtccagcc gtccagttca tgcatggcgc
     2941 gcccctgcgt cgtcgccggc cgccggcggg cgtggctcgg cgtacaggct gatggccacc
     3001 gccaccgcga tcgccgccac gatgttgagc cggtacggca gcccgaacag caccaccgcc
     3061 gccgccgccg cggtgatggc cgacagcgcg cgcaggcgcg tggtcaccat cgaacaggcc
     3121 accgccagca gcgccagcgt gccggcgaac tccagccccc agcgcgacgg cagcgcggcg
     3181 ccgagaaaga tgcccagcat gctgagcgac tgccagaaca cccagttggt gacgttgccg
     3241 ccccacagat agctgccctg ggcgcgccgc tgaccctcgg tttcgggcgg gcgcggaaag
     3301 cgccgggtga acagcacata ggtcacgtcg cccgtgaagt agcccaggta caagcgtttg
     3361 aatctaggca gatgcatgaa atatggccgc agatgaaggc tgaagactac aaatcgcaga
     3421 ttcacgcaga acgccgccgc cagcaccacc cacagcggcg catcggcggc aatgagcgga
     3481 atggcggcca gctgggcgct gccggcaaac accagcaagg acatcagcac gctttcggtg
     3541 atcgacatgc cgctcttgac catggccacg ccggtcatga aggcccaggc cgtcaggccg
     3601 atggaaacgc ccgccatctc gcgcgcaccg acccagaaca gcgggtgccg cgccgtgcgc
     3661 gcccaggtat cgacgcgccg cgccagccag gcgcccagcc ggccacgcgc cggcagctcg
     3721 cccggatcgg gcggcagcgg catgatcatg gcgcgccacc cgtgccttcg cccaggcact
     3781 ggccgggcgc cagcgcgaag ccgcgcagga actcgcgcgc cggtaggcgc ttgccgcccg
     3841 ggcgctgcag ctcggtcagg cgcagcacgg cgtccgcgca ggccaccgcg atgccgcctt
     3901 cgtctacggc aagaatctgg ccgcagcgcg catcggacgg gcgcgagcag ctatcgatct
     3961 cggagctcca cagcttgacc acggtgtcgc ccagctggct gctggcgccg gggaaggggt
     4021 cgaaggcgcg gatgcgccgc tcgatctgtg ccgccggctg gtgccagtcg atggcggcct
     4081 cgtgcttgtc gatcttgtgg gcgtagcaaa cgccctcggc cggctgcggc gtcggccgca
     4141 ggcgaccctg cgcggcctgc tgcagcgcct gcacgatcag ctgcccgccc agcgccgcca
     4201 gttcgtcgtg caggctggcg gtggtggtgc gcgggccgat cggcagcgcc tgcgccagca
     4261 gcatgtcgcc ggtgtccagg ccggcgtcca tctgcatgat ggtgacgccg gtctgcgcgt
     4321 cgccggcctc gatggcgcga tggatcggcg ccgcgccgcg ccagcgcggc agcagcgagg
     4381 catggatgtt gaggcaaccg aaggcctggc cggtggcgct gcccgccgag ggggcgctcg
     4441 caccttgggg cggcccggcg gtgctcgggt cacggccccc acgctccacg gctgcgcctg
     4501 ttgcgctgcc ccccgagggg gcgctcgcac cttggggcgg cccggcggtg ctcatgtcat
     4561 ccagcaccca ctgcggcagg atcagcccgt aggcggccac caccatcgcg tcggcgcggg
     4621 cggcgcgcag ggccgcgcgg gcggcggcgg cgtcttcggg gtacttgccg tccaggcgca
     4681 ggctgcgcgg ctgggcgacg gcgatgccgt gctgcagcgc cagctgcttg accggcgagg
     4741 cctgcagctt catgccgcgg ccggccgggc ggtcgggctg ggtcagcacc agcggcacct
     4801 cgaacccggc cgccagcagc gcctgcaggg ccacgcgggc aaactcgggg gtgccggcaa
     4861 agatcacgcg catgctcatg tttcgatagc ttttggcgct ggcgggttgg cgctttggtg
     4921 gcgattcaat ggcacggaac ggtcagcgcg gctcgggcgg gtcgggcagg ttttcgtcgc
     4981 cggcggacca gccgcgcacc acgccggcgc ggtcgatgtc gatgaacagc aggcgccagg
     5041 tcggcccgtc caggtagcgc cagacccaga tgtcgccgtc gaaccggtgc accgccgtca
     5101 cctgggccgg ccggccgtat tcgcgcagca cgtcctcgcg cgtccagcgg tcgggctgga
     5161 tgcgctggcc aaaccgcgct tcgctgagcg cctgctcgac gcgcaccacg cggccctggg
     5221 cgtccaggtc cagattgaac acctggcggc cggccggttg ctgcgaatac tgccagcgct
     5281 cgcccgcctg ggccgtggcc gggtagcgcg ccgtgggcgg acccaggcgc tgcagcacct
     5341 gcgccgccgg ggtgccgggc gccacccgat ccgggttgag cagcgccgca caagccccca
     5401 gcagcagcgc cccaagcgcc gccggcaagg ccttcaggac acgccggatg acgaatgcat
     5461 cagcctttgc cgcgcagtgg gcgctcacgc cggcacccgc gcctggtcgg ctttttcctg
     5521 tcgctcggct ttcagcagtt tggtcttgat gcggccgcgc ttgagcggcg acaggtattc
     5581 gacgaacacc ttgccgagca ggtggtccat ctcatgctgg atgcacaccg ccagcaggcc
     5641 ctcggcctcg atctcgcgcg cctcgccggc cgcgtccagc gcgcgcacac gcacgcgctc
     5701 ggcgcgctcg acgccgtcgt agatgccggg caccgacagg cagccctcct cgccgcgcac
     5761 gcgctgctcg ctggcccaca gcaactcggg gttgatcagc accaggcgct ggttgcgctc
     5821 ttccgacacg tcgatcacga tcagccgctc gtgcacgtcg acctgggtgg ccgccaggcc
     5881 gatgcccttg gcggcgtaca tggtctcgaa catgtcgtcg atcagcgcct gcacgcgcgc
     5941 atcgaccacc tgcaccgggc gagccacctt gtgcaggcgc gggtcggggt aacggagaat
     6001 gggaagctgg gccatggaat tcttgcctcg gatgtggcga ttttcgctac ttttcgcagt
     6061 tcgcgctgcc tcgggtgagt gatttcgttg ccggatcaag ggcttgaatg cagaatgtcc
     6121 ggtgacttgt caagttggca gctgcccccg gcaaaacaat gaaacacact tttcctggtg
     6181 ccacgcgcgc actgatcgcg cccctcggcc tgctgacggc ggccgtgctc agcgtcccgc
     6241 ccgccgccgc gcagtcggcc tcacgcctgc cggtgacgcc ggcgcagcgc gccaccgccc
     6301 agcaggtggc ggcgcagggc gtgccgctgt cggcgctggc gcccgacgcg cccgaccagt
     6361 acacggtgcg ccgcggcgac accctgtggc gcatctccag cctgttcctg aagtcgccct
     6421 ggcgctggcc cgagctgtgg ggcatgaacc tgcaggacat ccgcaacccg cacctgatct
     6481 acccaggcca gcagctgtac ctggagcgcc tgggcgaccg cgccttcctg cgcgcgcgcc
     6541 gcggcgccga cggcgaagtc ggtgacaccg tgcgggtgtc gccgcgcaac cgcatcgaga
     6601 tgttgggcac cggcccgctg cccacgctgg agccgcacct gatcgagccc ttcctgaccg
     6661 agccgctggt ggtcgacgac gacagcttcc agcgcgcacc ccgcatcgtc gcgctggcca
     6721 accagagccg cgtgctggtg gccaagggcg accgcgccta cgtgcgcggg cccgccgatg
     6781 cccccctgct gaaacaggcg ggcgacccca ccgaattccg cgtgttccgc aatgccaagc
     6841 cgctgaaaga cccggtgacc ggcgaaaccc tgggctacga aggccactac gtcggtcagg
     6901 ccaccctggt gcggggcgaa tccgaagccg aggaagacgc cggcgcggcg cccgccgtgc
     6961 gccccacgcg ctggcccgcc aacggcgaag tgcagagcga accgcgccgc gccgccgccc
     7021 cgcagccgcg cgccctgccc gtgcccgcca cggtcgacgt gatcagcagc aaggaagaaa
     7081 tgcgcgccgg cgaccgcctg ctgcccgagc cgccgcgcga gtaccgcaac tacgtgccgc
     7141 gcgtgcccga ggtgccggtg gacgcgcgcg tggtcgccgt gccgggcagc gcggtgcgct
     7201 acgccggcca gaaccaggtg gtggtgatca acaagggcct gcaggacggc atcgaaagcg
     7261 gtcatgtgct ggcgctgctg agcaccggac cgaagctggt ggacaagacc gacgaggcgc
     7321 gcggcctgat gaagctgccc gacgagcgca acggcatcgc catggtgttc cgtccgttcg
     7381 agcgcgtctc ctacgtgctg gtgatgga
//
LOCUS       DAOQTZ010000121         7136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_11579_length_7136_cov_10.689451, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000121 DAOQTZ010000000
VERSION     DAOQTZ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11579_length_7136_cov_10.689451"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2476)
                     /gene="gspD"
                     /locus_tag="PKN51_11830"
     CDS             complement(<1..2476)
                     /gene="gspD"
                     /locus_tag="PKN51_11830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNT85830.1"
                     /translation="MPRFTPLPLVLACAGLLASCAATDAGPAHAPAAQVAAASPVVVA
                     PAPASAPPAPAAAAADGDTGSEPMLIRGTDRVVAPPPIAVNAPARGGTTSLKFESAPA
                     AEVVRVMLGDLLKVDYVVHPPLSGQITLTTRQPVSPDRALLLLEAALQANGTVMARDS
                     RGVYHVGAPEALRGIVGAPVLAEPGKPLPPGYGSVIVPLEYLGATEMANILRPMVAPD
                     AIVRVDTLRNLLVMRGTRAEAEGWLDLVRTFDVNLLRGMSVGVFQLKHSSAAEVEAAL
                     RLLSGGTTSGAAGAAAAAGAAGASTARPPAAAGRPAAAGTQAPAAAGQPAAPAGLGEA
                     SPLFGALRVIPIERINAVLVVTPRAAYLDEARYWIEQFDRPNLNSVEPQLFVYKVQNG
                     SADHLAELLNGIYGGTTQQSGFSGATGLAPGLTGVGASTNVNSSFASQFGANRNASQT
                     RAWGSTGGLGGTGGLLGGAAGGLGAAGGLGLSGGARGTLGAGTAGTAGAAQGPAVVTT
                     TLGQNVRVMADRINNTLLVHATPAEYERIAATLKRLDIQRAQILIEASIVEVTLGDGL
                     EYGLQWTFNGGLGDGRSGRGVIGAAAAGGTLAADGSTAAGISNAFTYTLRNAAGNISA
                     VLTALANKSLVKVMSSPSLMVLDNHTAAIQVGTQQPVNTGTTISGTSNITTTNIQYKD
                     TGVMLGVTPSVSAGDLVTLDIDQIVTDLGADSSSAATSGYPTFLQRQINSKVAVRSGE
                     TLVLGGLIKDSASSGKAGVPLLSSIPVVGALFGRHRNTADRTELLVILTPRVLRSDED
                     ARAISRELRDRMQGLLQGGAALPAPAR"
     gene            complement(2526..2717)
                     /locus_tag="PKN51_11835"
     CDS             complement(2526..2717)
                     /locus_tag="PKN51_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85831.1"
                     /translation="MRLLRPFVPLVLLVGSALVACGQRDAPASDSAAPASPPSSAAQR
                     GSFGGGAPKAPASAASAAH"
     gene            complement(2763..3503)
                     /locus_tag="PKN51_11840"
     CDS             complement(2763..3503)
                     /locus_tag="PKN51_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85832.1"
                     /translation="MNRAGTIVYLAAVGLLTFVLAALWLAPGPQARWRQWQAPAPQAP
                     QLDDVQAAQLRPNPAATANYPGVVQRPLFSPDRRPVAAASAAAPAAPPVAIEQVRLQG
                     LVAGPSLTGVMLEEQGKTRFVRVGETVADWKLERVQARAALFSRAGQQREIALKAVSP
                     EPSSGAANAPAQGRPRAPAAAPPTAAAPAAPAAAPAGVSAPPGDTTSAPRAPQPAAAG
                     APAARGGFGGGAGRPRPAAPAGTTNSAR"
     gene            complement(3500..4063)
                     /gene="gspM"
                     /locus_tag="PKN51_11845"
     CDS             complement(3500..4063)
                     /gene="gspM"
                     /locus_tag="PKN51_11845"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNT85833.1"
                     /translation="MTTPLARPRPEALWLLIAGALLLALLAWASWTVVAKYRQASAVL
                     AEIEPRHARLAGLLQNQELLAQTDSALQANLIEHVGTAEEDPGQTGNAALQRVRELAG
                     GRGLRVASSQTTAPRDDNGFDRIGLSLRVEGDWPALVALLRELAQQRPALYIHSVQLG
                     IQGFGHQGAPQTLFGNLELYVLKERRP"
     gene            complement(4060..5142)
                     /locus_tag="PKN51_11850"
     CDS             complement(4060..5142)
                     /locus_tag="PKN51_11850"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNT85834.1"
                     /translation="MTSTTADRHRPFSLDPAQWPRQWRAAAMLALDGPLLRGLRPASR
                     VALRHADGQTRVWRLVRGQAHAVPGAPGGAELPALQLPAERVLERRLLLPPLAPDDLA
                     QAVQLDVAASSPFGAAQTVHGWSVRRAADQLVQVDVAIASRPQVEQALQAAGFDPAQA
                     PEVWVLPPAADGASVQPVVLRGFGEDRRERLVRQGLTRRLWLLALLLALLAALVITPT
                     ALVRARAQQAQQAFAALQKQAAPQLAQREALMQRLARLQAVGQVYQQQLALPPVLDLL
                     TRALPDGAWLTGLRLEGTKLVLNGQADDAAALVQRLAREPGAHDVRLASPATRGAGAT
                     KETFIIELRLEPRRHGPVQIGEGAAS"
     gene            complement(5162..6046)
                     /locus_tag="PKN51_11855"
     CDS             complement(5162..6046)
                     /locus_tag="PKN51_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNT85835.1"
                     /translation="MKVRLLHPPSRTAASAGMALIAVLWIVAALSLLVVGVSATVRQQ
                     IRFVGAERDQVSGQALGEAAIALALQQLQVAPERPRGIVSASVDYAGVPITVELAPLS
                     GLISLNGAAPELLAALLQVAGGLPAAQAQALAASLVEWREGRPELALTTDPAARQPRR
                     FEAVEDLLLVPGIDYGLYARLAPLVSADLGSLARVNPEAAPPGVLAVLAQGQGGAAQQ
                     YLRQRAGGQPGADTSGFNQAFIGTAGTSLYRLTAGVPLEAGKMLHLVQDVALVAGASR
                     GAPWRVLRSRRQIVLTPG"
     gene            complement(6051..6743)
                     /locus_tag="PKN51_11860"
     CDS             complement(6051..6743)
                     /locus_tag="PKN51_11860"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT85836.1"
                     /translation="MNAALGRRRGFTLVEVLIALVLLSLLMLVLTSALRSMGQVEARV
                     EQRIEAADDFRLATQLLGEVLGQASARRHLVQAAGGPAQAPFFEAGPEALAWIGVMPA
                     RFGLGGRHYLRLALEPGEGGARLVLRYAPWNGAPAFSAWGQAEARVLAWPVSALALRY
                     LEPASGQWSPQWPPPGLTTNALPPSLLPAAVELRLDGPAPAWPPLVVALRANYATDPS
                     AVLGGFGGGAPR"
     gene            complement(6740..>7136)
                     /locus_tag="PKN51_11865"
     CDS             complement(6740..>7136)
                     /locus_tag="PKN51_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT85837.1"
                     /translation="RQRGFSLLEILVAFAIAAMALGLLYQVSGNNARQAGGLLQHERA
                     MALAQSLLAAHETVPARGLDERGEGAGYGWYVHSRPYPTPAGNAAPQAARLHELRIDV
                     RWHDGTAERSFELSSLRPERRPQPGEVIR"
BASE COUNT          890 a         2434 c         2919 g          893 t
ORIGIN      
        1 gccgggccgg cgccggcagc gccgcgccgc cctgcagcag gccctgcatg cggtcgcgca
       61 gttcgcggct gatggcgcgc gcgtcttcgt cggagcgcag cacgcgcggg gtcaggatga
      121 ccagcagctc ggtgcggtcg gcggtgttgc ggtggcgccc gaacagcgcg cccaccaccg
      181 ggatggacga cagcaggggc acgccggcct tgccgctgct ggcgctgtcc ttgatcaggc
      241 cgcccagcac cagggtttcg cccgagcgca ccgccacctt ggagttgatc tggcgctgca
      301 ggaaggtggg gtagcccgag gtggcggccg aggacgagtc ggcgcccagg tcggtgacga
      361 tctggtcgat gtccagcgtg accaggtcgc ccgcgctgac cgagggcgtg acgcccagca
      421 tgacgccggt gtccttgtac tggatgttgg tggtggtgat attgctggtg ccgctgatgg
      481 tggtgccggt gttgaccggc tgctgggtgc cgacctggat ggcggcggtg tggttgtcga
      541 gcaccatcag cgaggggctg gacatgacct tgaccagcga cttgttggcc agcgcggtga
      601 gcaccgccga gatgttgccg gcggcgttgc gcagggtgta ggtgaaggcg ttgctgatgc
      661 cggccgcggt gctgccgtcg gcggccaggg tgccgccggc cgccgcggca ccgatgacgc
      721 cgcggccgct gcgaccgtcg cccaggccgc cgttgaaggt ccattgcagg ccgtattcga
      781 ggccgtcgcc gagggtgact tcgacgatcg aggcttcgat caggatttgc gcgcgctgga
      841 tgtccagccg cttgagggtg gcggcgatgc gttcgtattc ggccggggtg gcgtgcacca
      901 gcagggtgtt gttgatgcgg tcggccatga cgcgcacgtt ctgccccagg gtggtggtga
      961 ccaccgccgg cccctgcgcc gcgccggcgg tgcctgcggt gccggcgccg agggtgccgc
     1021 gcgcgccgcc gctcaggccc aggccaccgg ccgcgcccag accgccggcg gcgccgccca
     1081 gcagcccccc ggtgccgccc agaccgcccg tgctgcccca ggcccgcgtc tgcgaggcgt
     1141 tgcggttggc gccgaactgg ctggcgaagc tgctgttgac gttggtgctg gcgccgacgc
     1201 cggtgaggcc cggcgccagg ccggtggcgc cgctgaagcc gctttgctgg gtggtgccgc
     1261 cgtagatgcc gttgagcagc tcggccaggt ggtcggccga gccgttctgc accttgtaga
     1321 cgaacagttg cggctcgacg ctgttgaggt tggggcggtc gaactgctcg atccagtagc
     1381 gcgcctcgtc caggtaggcg gcgcgcgggg tgaccaccag cacggcgttg atgcgctcga
     1441 tggggatgac gcgcagggcg ccgaacaggg ggctggcttc gcccaggccg gccggcgcgg
     1501 ccggctggcc ggcggccgcc ggggcctggg tgccggccgc ggccggccgg cccgccgcgg
     1561 cgggggggcg ggccgtgctg gcgccggcgg caccggcggc ggccgcggca ccggcggcgc
     1621 ccgaggtggt gccgcccgag agcaggcgca gcgcggcctc gacctcggcg gccgagctgt
     1681 gcttgagctg gaacacgccg accgacatgc cgcgcagcag gttgacgtcg aaggtgcgca
     1741 ccaggtcgag ccagccttcg gcttcggccc gggtgccacg catgaccagc aggttgcgca
     1801 gggtgtcgac gcgcacgatg gcgtcgggcg cgaccatggg ccgcaggatg ttggccattt
     1861 cggtcgcgcc caggtattcg agcggaacga tgacgctgcc gtagccgggt ggcagcggct
     1921 tgccgggttc ggccagcacc ggggcgccga cgatgccgcg cagcgcttcg ggcgcgccga
     1981 cgtggtagac gccgcgcgag tcgcgcgcca tgacggtgcc gttggcctgc agcgcggctt
     2041 cgagcagcag cagggcgcgg tcggggctga cgggctggcg cgtggtgagg gtgatctggc
     2101 cgctgagcgg ggggtgaacg acgtagtcga ccttgagcag gtcgcccagc atgacgcgca
     2161 ccacttcggc ggcgggggcg gattcgaact tgaggctggt ggtgccgccg cgcgccgggg
     2221 cgttgaccgc aatgggcggc ggcgccacca cgcggtcggt gccgcggatc agcatcggct
     2281 cgctgccggt gtcaccgtcg gccgcggcgg cggcaggggc gggcggcgcg ctggcggggg
     2341 ccggggcgac gaccacgggc gacgctgcgg cgacctgggc cgcgggcgcg tgggccgggc
     2401 cggcatcggt ggcggcgcag gacgccagca ggccggcgca ggccagcacg aggggcaaag
     2461 gggtgaaacg gggcatgggc ttcggtgtcg atggggtcgc gaagacgggg cgggggctgc
     2521 gcaggtcagt gcgccgcgct ggcggcgctg gcgggcgcct tgggcgcgcc gccgccgaaa
     2581 ctgccgcgct gggcggcact ggaaggcggg ctggcgggcg cggccgaatc ggaggcgggg
     2641 gcgtcacgct ggccacaggc caccagggcg ctaccgacca gcaacacgag cggaacgaag
     2701 gggcgaagca ggcgcatggc agtacgaagc ggtgagtgag gatgggcagg ggcggtccag
     2761 gatcatcgcg ccgagttggt ggtgccggca ggcgcggcgg gcctggggcg gccggcacca
     2821 ccgccgaagc cgccgcgggc ggccggcgca cccgcggcag cgggctgggg cgcgcggggc
     2881 gccgacgtgg tgtcgccggg aggggcggac acgcccgccg gcgcagcagc aggggcggca
     2941 ggggcggcag cggttggggg cgccgcggcg ggcgcgcgcg ggcgtccctg cgctggcgca
     3001 ttggcggcac cggacgacgg ctcggggctg accgccttca gggcgatctc gcgctgctgg
     3061 cctgcacggc tgaacagggc ggcgcgggcc tgcacgcgct cgagcttcca gtcggcgacg
     3121 gtttcgccca cacgcacgaa gcgggtcttg ccctgctctt ccagcatgac gccggtcaag
     3181 gatggtccgg ccaccaggcc ttgcagccgg acctgttcaa tggccacggg gggcgccgcc
     3241 ggcgcggcgg cgctggcggc ggcgacgggg cggcggtcgg ggctgaacag cgggcgctgg
     3301 acgacgccgg ggtagttggc ggtcgccgcc gggttgggcc gcaactgggc ggcctgcacg
     3361 tcgtccagct ggggggcctg gggtgcgggc gcctgccact ggcgccagcg cgcctgtgga
     3421 ccgggcgcca gccacagcgc cgccagcacg aaggtcagca ggccgacggc ggcaaggtag
     3481 acgatggtgc cggcgcggtt catggccgac gctccttgag cacgtagagc tccaggttgc
     3541 cgaacagggt ctggggcgcg ccctggtggc caaagccctg gatgccgagt tgaacgctgt
     3601 ggatgtagag cgcggggcgt tgctgcgcca gctcgcgcag cagcgccacc agggccggcc
     3661 agtcgccttc gacgcgcagc gacaggccga tgcggtcgaa gccgttgtcg tcgcgcgggg
     3721 cggtggtctg gctgctggcc acacgcaggc cgcgcccgcc ggcgagttcg cgcacgcgct
     3781 gcagggcggc gttgccggtc tggccagggt cttcctcggc cgtgccgaca tgttcaatca
     3841 aattggcctg cagcgcgctg tcagtctgcg ccagcagctc ttgattttgt agcaaaccgg
     3901 caagccgggc atggcgcggt tcgatctcgg ccagcacggc gctggcctgg cggtacttgg
     3961 ccaccacggt ccaggacgcc caggccagca gcgccagcag cagcgcgccg gcgatcagca
     4021 gccacagggc ttcggggcgt gggcgggcga gcggggtggt catgaggcgg cgccctcccc
     4081 gatctgcacc gggccatggc ggcgcggttc gagccggagt tcgatgatga aggtctcctt
     4141 ggtggcgccg gcgccgcggg tggcgggcga ggccaggcgc acgtcgtggg cgccgggttc
     4201 gcgcgccagc cgctgcacca gggcggcggc gtcgtcggcc tggccgttga gcaccagctt
     4261 ggtgccttcc aggcgcaggc cggtgagcca ggcgccgtcg ggcagggcgc gggtgagcag
     4321 gtccagcacc ggcggcaggg ccagttgctg ctggtagacc tggccgacgg cctgcaggcg
     4381 cgccagccgc tgcatcagcg cctcgcgctg ggccagctgg ggcgcggcct gcttttgcag
     4441 cgcggcaaac gcctgctggg cctgctgggc gcgcgcccga accagcgccg tgggggtgat
     4501 gaccagggcc gccagcagcg ccagcagcag cgccagcagc cacagccggc gtgtcaggcc
     4561 ctggcgcacc aggcgttcgc gccggtcttc gccgaagccg cgcagcacca ccggctgcac
     4621 gctggcgccg tcggccgccg gcggcagcac ccagacttcg ggcgcctgcg cggggtcgaa
     4681 gccggccgcc tgcagcgcct gctcgacctg cgggcgcgag gcgatggcga cgtcgacctg
     4741 gacgagttga tcggcggcgc ggcgcaccga ccagccgtgc acggtctggg cggcgccgaa
     4801 cgggctggag gcggccacgt ccagctgcac ggcctgcgcc agatcgtcgg gcgccagggg
     4861 gggcagcagc aggcggcgct ccagcacgcg ctcggccggc agttgcaggg ccggcagctc
     4921 ggccccgccg ggggcacccg gcacggcatg ggcctggccg cgcaccaggc gccagacgcg
     4981 ggtctgaccg tcggcgtggc gcagcgccac gcggctggcc ggccgcaggc cgcgcagcag
     5041 cgggccgtcc agcgccagca tggcggcggc gcgccactgg cgcggccact gggcggggtc
     5101 gaggctgaag ggacggtggc ggtcggcggt ggtggaggtc atgcggggga aaggtccggg
     5161 ctcatccggg tgtcaggacg atctggcggc ggctgcgcag cacgcgccag ggcgcgccgc
     5221 gcgatgcgcc ggccaccagg gccacgtcct ggacgaggtg cagcattttg cctgcttcca
     5281 gcggcacgcc ggccgtgagg cggtaaagag acgtgcctgc ggtgccgatg aacgcctgat
     5341 tgaaaccgct ggtgtcggcc ccgggctgac cgccggcgcg ctggcgcagg tattgctggg
     5401 cggcgccgcc ctggccctgc gccagcaccg ccagcacgcc aggcggcgcg gcttcggggt
     5461 tgacgcgggc caggctgccg aggtcggcac tgaccagcgg cgccagccgg gcgtacaggc
     5521 cgtagtcgat gccgggcacc agcagcaggt cctcgacggc ttcgaagcgg cgcggctggc
     5581 gggcggcggg gtcggtggtg agggccagct cgggtcggcc ttcgcgccat tcgaccaggc
     5641 tggcggccag cgcctgggcc tgggctgccg gcaggccgcc ggccacctgc agcagcgccg
     5701 ccagcagctc gggcgcggcg ccgttgagcg agatcaggcc gctgagcggc gccagttcga
     5761 cggtgatggg cacaccggcg tagtcgacgc tggcgctgac gatgccacgc gggcgctcgg
     5821 gcgcgacctg gagctgctgc agcgccagcg cgatggcggc ttcgcccagc gcctggccgc
     5881 tgacctggtc gcgttcggca ccgacgaacc ggatctgctg gcgcacggtg gcgctgacgc
     5941 cgaccaccag caggctgagg gcagctacga tccacagcac ggcgatcagc gccatgccgg
     6001 cgctggcggc ggtccgggat ggggggtgca ggaggcggac cttcatggca tcaacgcggt
     6061 gcaccgccac cgaagccgcc cagcacggcc gagggatcgg tggcgtagtt ggcgcgcagc
     6121 gccaccacca gcggcggcca ggccggtgcc gggccgtcca ggcgcagttc gacggccgcc
     6181 ggcagcaggc tgggcggcag ggcgttggtg gtcaggccgg gcggcggcca ctgcgggctc
     6241 cactggccgc tggccggctc caggtagcgc agcgccagcg cgctgaccgg ccaggccagc
     6301 acgcgcgcct cggcctgacc ccaggcgctg aaggcgggcg cgccgttcca gggggcgtag
     6361 cgcagcacca ggcgcgcgcc gccctcgccc ggctccagcg ccaggcgcag gtaatgcctg
     6421 ccacccaggc cgaagcgcgc cggcatgacg ccgatccagg ccagcgcctc gggcccggcc
     6481 tcaaagaacg gcgcctgtgc cgggccgccg gccgcctgca ccaggtggcg gcgcgccgac
     6541 gcctggccca gcacttcgcc cagcaactgg gtggcgaggc gaaagtcgtc ggcggcctcg
     6601 atgcgctgct cgacgcgcgc ttcgacctgg cccatgctgc gcagcgcgct ggtgagcacc
     6661 agcatcagca gcgacagcaa gaccagggcg atcagcacct cgaccagggt gaagccgcgg
     6721 cggcggccga gcgcggcgtt catcggatca cctcacccgg ctgcggccgt cgctcggggc
     6781 gcaggctgct gagctcgaag ctgcgctcgg cggtgccgtc gtgccagcgc acgtcgatcc
     6841 gcagctcgtg caggcgcgcc gcctgcggcg ccgcgttgcc ggccggcgtg gggtaggggc
     6901 ggctgtgcac gtaccaaccg tagccggcgc cttcgccgcg ctcgtccagc ccacgcgccg
     6961 gcacggtttc gtgggcagcc agcagcgact gcgccagcgc catggcgcgc tcgtgctgca
     7021 gcagaccgcc cgcctggcgc gcgttgttgc cgctgacctg gtagagcagg cccagcgcca
     7081 tggcggcaat ggcgaaggcg accaggattt cgagcagcga aaagccgcgc tggcgc
//
LOCUS       DAOQTZ010000122         7076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_11716_length_7076_cov_13.329868, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000122 DAOQTZ010000000
VERSION     DAOQTZ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11716_length_7076_cov_13.329868"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PKN51_11870"
     CDS             complement(<1..1112)
                     /locus_tag="PKN51_11870"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNT85838.1"
                     /translation="MARPLNPGHERRGLLASTALALLLHALALALLGPLLRPPSILRQ
                     VAPPFYTRTITPEAPPAAPQQAAPVARVSKPKRPVARIQKAPAAPVSVAPQAMAPASA
                     TPDTADADAPLASEEPQAPSPAASAALAESPAPDESLAARDPGGQDGAAAPPAGAARA
                     AEPGFLADWPGDTRLTYTLGGNYRGELHGSARVLWQREGTRYHTAVQLDVGLLLSMHL
                     TSQGDITATGLQPAVYEEQVRQRRRGVRLDEDVRLHNGQRVARPAGVQDAASQFVELS
                     HRLATGQLSTEPGTAIPLWLARPGGVDEWTYDVVGEDTLHLPRLGAVRALHFRPRPLA
                     KPRGPIHAEIWFAPSLQYLPVRIRITQGPDTFMDLLA"
     gene            complement(1097..2893)
                     /locus_tag="PKN51_11875"
     CDS             complement(1097..2893)
                     /locus_tag="PKN51_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HNT85839.1"
                     /translation="MSKALAATAPAPVRHDVRVASLHGHRYAVCLTIARPAAMQRVSL
                     PAWIPGSYLIREFARHLLPLHATQGGTARACRQIDKSTWDIDCDPRRPLVLRYEAYAF
                     DASVRTAWLDGERGFFNGTSLLLRVHGQEQVTHELCVHAPPQRRDWQLATAMTPVQVD
                     ARGFGRWRADDYDELVDHPVTLGAFWSGSFKAGGVPHRLVVTGAPPAFDGARLLRDTQ
                     KICETAIRLWHGAKAPPQRHYLFMLNAVDDGYGGLEHRASTALICGRRDLPRHDAPAS
                     AAQVGDGYLTLLGLISHEYFHTWNVKRLRPAEFTRYDYQQENYTRLLWFFEGFTSYYD
                     DLLLRRAGLIDDAAYLKLLTKTVNQVQQAPGRHVQSVAQASFDAWVRYYRPDENTPNS
                     TISYYTKGALVALCLDLTLRREGRATLDDVMRAVWQRCRGGPMTEADFADTLADIGGR
                     RFDAEIAAWVHGTDELPLRELLQAQGVQVLEEPSQLAQTLGLRLGDGPGLTLKVVLDG
                     GPAAAAGLAAGDEWLGIELPARTRRSDASGWRLSRLDDLPLYAGAARQIVALVARDQR
                     LLRLPLTLPQPSTTWRLAPPVSADAAASWPGR"
     gene            complement(2900..3616)
                     /locus_tag="PKN51_11880"
     CDS             complement(2900..3616)
                     /locus_tag="PKN51_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNT85840.1"
                     /translation="MTPVALLRPALAALCLTLPLLAAAQESTIRKNLAERIPALSQID
                     EISKTPMPGLFEVRVGTDVLYTDAEGNFLLHGSLLDARNGRNLTEERVNKLLAVDFKT
                     LELKNAITMVRGNGKRKLAVFEDPNCGYCKRFEKDLQKVDNVTVHLFLIPILGEDSVN
                     KSRQIWCAADRAKSWNDWMLRDVAPKGKAICNTEALTANLEFANKHRITGTPTLIFED
                     GSRVPGAIGAVQLEERLARR"
     gene            complement(3710..4840)
                     /locus_tag="PKN51_11885"
     CDS             complement(3710..4840)
                     /locus_tag="PKN51_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNT85841.1"
                     /translation="MPATHDVCIRGAGIVGRSLALLLARERLRVALVAPPASDTPRPP
                     DVRAYALGPAARALLDTLRCWPDERAATPVLEMQVFGDQLSQVRFSAAEQGVPALNWI
                     VDVPTLEQQLAEAVRYQSGIELVAAPLAAPLTVVCEGRASATRTELGVDWQITRYPQQ
                     AIATRLECELPHAQVARQWFTPEGEILALLPLDGAQGRQVAVVWSVRSEHLAELLALD
                     DAAFAARLHAVSGGALGALHLSGPRAHWPLLRAQAARWVGDMPGRPGERFALAGDAAH
                     AMHPLAGQGLNVGLGDVAELARQLATREAWRAPGDPKLLRRYARARQGDWLRMSLATD
                     ALQQLFSRPEAGAGALRNWGMAAFDRSGPLKRRVAALAMGTA"
     gene            4975..5847
                     /locus_tag="PKN51_11890"
     CDS             4975..5847
                     /locus_tag="PKN51_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNT85842.1"
                     /translation="MSTAHADLQARLSGLYVHPVKSCAGLAVTEARLMDTGLDLDRAW
                     MVVDAQGRFVTQRELPRMALVRPQLRALDVVLRAPGMLALHLGMNEVESPTRVQVWGD
                     ELAAWDMGQVAAQWLSDFLGRPGLRLARFDPEVTRLASRRWSGDIEAPIEFADGFPLL
                     LASQASLDELNARLGAAGHPPVGMERFRPNLVLDGVSAQDEDRLAELRITTAEGRQVV
                     LRPVKPCARCPIPNVDPASAAIHPEVLATLSTYRANPVLEGAVTFGMNCVIVQGAGAT
                     LRVGDAVAADWRFE"
     gene            5984..>7076
                     /gene="ychF"
                     /locus_tag="PKN51_11895"
     CDS             5984..>7076
                     /gene="ychF"
                     /locus_tag="PKN51_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNT85843.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNTGVV
                     EVPDPRLAQLAAIVKPERVLPAIVEFVDIAGLVAGASQGEGLGNQFLAHIRETDAIVN
                     VVRCFEDPNVIHVAGKVDPIADIEVIQTELCLADLGTVEKALQRYTKAAKSGNDKEAA
                     AMVKLLGPLQAALNEARPVRTLALSKEEQALLKPLCLITAKPAMFVGNVAEDGFDNNP
                     LLDRLNAYAASQNAPVVAICAKMEADMAEMSDEDKAIFLEEMGQSEPGLNRLIRAAFK
                     LLGLQTYFTAGVKEVRAWTIHIGDTAPQAAGVIHGDFERGFIRAQTIAFDDYIQYQGE
                     QGAKDAGKMRSEGKDYVVKDGDVMNFLFNV"
BASE COUNT          977 a         2432 c         2642 g         1025 t
ORIGIN      
        1 gccagcaggt ccatgaaggt gtccggcccc tgggtgatgc ggatgcgtac gggcaggtat
       61 tgcaggctgg gggcgaacca gatttcggcg tggatggggc cgcgcggctt ggccagcggg
      121 cgcggcctga agtgcagggc gcgcacggcg cccaggcgcg gcaggtgcag ggtgtcttca
      181 ccgacgacgt cgtaggtcca ttcgtccacg ccgccggggc gcgccagcca caggggaatg
      241 gcggtgcccg gctcggtgct cagctgcccg gtggccaggc gatggctgag ttcgacgaac
      301 tggctggcgg cgtcctgcac gccggccggg cgcgccaccc gctggccgtt gtgcaggcgc
      361 acgtcctcgt ccagccgcac gccgcggcgg cgctggcgca cctgctcttc atagaccgcc
      421 ggttgcaggc cggtggcggt gatgtcgccc tggctggtca ggtgcatgga cagcagcagg
      481 ccgacgtcga gttgcaccgc cgtgtggtag cgcgtgcctt cgcgctgcca cagcacccgg
      541 gcgctgccgt gcagctcgcc gcggtagttg ccgcccaggg tgtaggtcag gcgggtgtcg
      601 ccgggccaat cggccaggaa cccgggctcg gcggcgcggg cggcgccggc gggtggggcg
      661 gcggcgccgt cctggccgcc ggggtcgcgg gcggcgaggc tttcgtccgg cgccggcgac
      721 tcggccagcg cggccgaagc cgcgggtgag ggcgcctggg gttcttcgct ggccagcggc
      781 gcatcggcgt ctgccgtgtc gggggtggcg gatgccggcg ccatcgcctg gggtgctact
      841 gaaacaggag ctgctggcgc tttctggatg cgcgctacag gccgttttgg cttggaaacc
      901 cgggccacgg gcgcagcctg ctggggtgcc gctgggggcg cctcgggtgt gatggtgcgg
      961 gtgtagaagg gcggcgccac ctggcgcagg atgctgggcg ggcgcagcag cgggcccagc
     1021 agcgccagcg ccagcgcgtg cagcagcagc gccagcgcgg tgctggccag caggccgcgc
     1081 cgttcgtgcc ctgggttcag cggccgggcc acgacgcggc ggcgtcggcg ctgaccgggg
     1141 gcgccagccg ccaggtggtg gagggctgcg gcagggtcag cggcaggcgc agcaggcgct
     1201 ggtcgcgcgc caccagggcg acgatctggc gcgccgcgcc ggcgtacagc ggcaggtcgt
     1261 cgagccggct caggcgccag ccgctggcgt cgctgcgccg ggtgcgggcg ggcagctcga
     1321 tgcccagcca ttcgtcgccg gcggccaggc cggcggcggc ggccgggccg ccgtcgagca
     1381 ccactttgag cgtcaggccg gggccgtcgc ccaggcgcag gcccagcgtc tgcgccagtt
     1441 gcgagggctc ctccagcacc tgcacaccct gcgcctgcag cagctcgcgc agcggcagct
     1501 cgtcggtgcc gtgcacccag gcggcgattt cggcgtcgaa acggcgcccg ccgatgtcgg
     1561 ccagggtgtc ggcgaagtcg gcctcggtca tcgggccgcc gcggcagcgc tgccacacag
     1621 cccgcatcac gtcgtcgagc gtggcgcggc cttcgcggcg cagcgtgagg tccaagcaca
     1681 gcgccaccag cgcgcccttg gtgtagtagc tgatggtgct gttgggcgtg ttttcgtccg
     1741 gccggtagta gcgcacccag gcgtcgaagc tggcctgcgc caccgactgc acgtggcgcc
     1801 ccggcgcctg ctgcacctgg ttgacggtct tggtcagcag cttgaggtag gcggcgtcgt
     1861 cgatcaggcc ggcgcggcgc agcagcaggt cgtcgtagta gctggtgaag ccttcgaaga
     1921 accacagcag gcgcgtgtag ttttcctgct ggtagtcgta gcgggtgaat tcggccgggc
     1981 gcaggcgctt gacgttccag gtgtggaagt actcgtggct gatcaggccc agcagcgtca
     2041 ggtagccgtc gcccacctgg gcggcgctgg ccggcgcgtc gtggcgcggc agatcgcgcc
     2101 ggccacagat cagggccgtg ctggcgcggt gttccaggcc gccgtagccg tcgtcgacgg
     2161 cgttcagcat gaacaggtag tgccgctgcg gcggcgcctt ggcgccgtgc cacaggcgga
     2221 tggcggtttc gcagatcttc tgcgtgtcgc gcagcaggcg cgcgccgtcg aacgccggcg
     2281 gcgcgcccgt gaccaccagc cggtgcggca cgccgccggc cttgaagctg ccgctccaga
     2341 aggcgcccag cgtcaccggg tggtcgacca gctcgtcgta gtcgtcggcg cgccagcgac
     2401 cgaagccgcg cgcgtcgacc tgcaccgggg tcatggcggt ggccagctgc cagtcgcgcc
     2461 gctggggcgg agcgtgcacg cacagctcgt gcgtgacctg ttcctgcccg tgcacgcgca
     2521 gcagcaggct ggtgccgttg aagaagcccc gttcaccgtc cagccaggcg gtgcgcaccg
     2581 aggcgtcgaa ggcataggcc tcgtagcgca gcaccagcgg ccggcgcggg tcgcagtcga
     2641 tgtcccaggt ggacttgtcg atctggcggc aggctcgcgc ggtgccgccc tgcgtggcgt
     2701 gcagcggcag caggtggcgg gcgaattcgc ggatcaggta gctgcccggg atccaggccg
     2761 gcagcgatac gcgctgcatg gctgccgggc gggcgatggt caggcacacc gcgtagcgat
     2821 ggccgtgcag gctggcgacg cgcacgtcgt ggcgcacggg cgccggtgcg gtggcggcaa
     2881 gggccttgct cacggcggtt cagcgccggg ccaggcgctc ttcgagctgc accgcgccaa
     2941 tggcgccggg cacgcgcgag ccgtcttcga agatcagcgt cggcgttccg gtgatgcggt
     3001 gcttgttggc aaactccagg ttggcggtca gcgcctcggt gttgcagatc gccttgccct
     3061 tgggggcgac gtcgcgcagc atccagtcgt tccagctctt ggcgcggtcg gcggcgcacc
     3121 agatctggcg cgacttgttg accgagtctt cgcccaggat cgggatcagg aacaggtgca
     3181 cggtgacgtt gtcgaccttc tgcaggtctt tttcgaagcg cttgcagtag ccgcagttgg
     3241 ggtcttcaaa caccgccagc ttgcgcttgc cgttgccgcg caccatggtg atggcgtttt
     3301 tcagctccag cgtcttgaag tccaccgcca gcagcttgtt gacgcgctct tccgtcaggt
     3361 tgcggccgtt gcgggcgtcc agcaggctgc cgtgcagcag aaaattgccc tcggcgtcgg
     3421 tgtagagcac gtcggtgccc acgcgcacct cgaacaggcc gggcatcggc gtcttgctga
     3481 tctcgtcgat ctggctcagc gccgggatgc gttcggccag gttcttgcgg atggtcgatt
     3541 cctgggccgc ggccagcagc ggcagcgtca ggcacagcgc cgccagggcg gggcgcagca
     3601 gggcaacagg tgtcatgtcg gaaagctcgg caaaggttca aacggggtca cgacggtcac
     3661 aggcgcgaca ggtgaatgag gtgggcatga ggggcgcgtt cacgccctct caggcggttc
     3721 ccatcgccag ggcggcgacg cggcgcttga gcgggccact gcggtcgaac gccgccatgc
     3781 cccagttgcg cagcgcgccg gcaccggcct cggggcggct gaacagttgc tgcagcgcgt
     3841 cggtggccag gctcatgcgc agccagtcgc cctgccgcgc gcgtgcgtag cggcgcagca
     3901 gcttcggatc gcccggcgcg cgccaggcct cgcgcgtggc cagctggcgc gccagctcgg
     3961 cgacgtcgcc cagaccgacg ttcagaccct gaccggccag cgggtgcatg gcgtgggcgg
     4021 cgtcgccggc gagggcaaag cgctcgcccg gccggcccgg catgtcgccg acccagcgcg
     4081 ccgcctgcgc gcgcagcagc ggccagtggg cgcgcggacc actcaggtgc agggcgccca
     4141 gggcaccgcc gctgacggcg tgcaggcgcg cggcgaaggc ggcgtcgtcc agcgccagca
     4201 gctcggccag gtgctcgctg cgcaccgacc agaccaccgc cacctgccgg ccctgcgcgc
     4261 cgtccagcgg cagcagggcc aggatctcgc cctcgggcgt gaaccactgg cgcgccacct
     4321 gagcatgcgg cagctcgcat tccagccggg tggcgatggc ctgctgcggg tagcgggtga
     4381 tctgccagtc cacgcccagc tcggtgcggg tggcgctggc gcggccttcg cacaccaccg
     4441 tcagcggcgc ggccagcggg gccgcgacca gctcgatgcc gctctggtag cgcaccgcct
     4501 cggccagctg ctgctccagc gtcggcacat cgacgatcca gttgagcgcc ggcaccccct
     4561 gttcggcggc cgaaaagcgc acctgactga gctggtcgcc gaacacctgc atctcgagca
     4621 ccggggtggc ggcgcgttcg tccggccagc agcgcagggt gtcgagcagg gcgcgcgcgg
     4681 ccggaccgag ggcgtaggcg cgcacgtccg gcgggcgcgg cgtgtcggag gcgggcggtg
     4741 ccaccagcgc cacgcgcagg cgctcgcgcg ccagcagcag cgccagcgag cgaccgacga
     4801 tgccggcgcc gcggatgcag acgtcatggg tagcgggcat gcggggattg taggcgccgc
     4861 ccccacgcgg cgcggtcggc gcagtgcggc ccgcggccgg ccgcggcgcg cgatggcgga
     4921 caatggcgcg ccttcttcac gccttgccgc gccgccgggc gcgcccgcca gcccatgtcc
     4981 accgcccacg ccgatctgca agcccgcctg tcgggcctat acgtccaccc cgtcaaatcc
     5041 tgcgccggcc tggcggtgac cgaggcgcgg ctgatggata ccgggctgga cctggaccgc
     5101 gcctggatgg tggtggacgc gcagggccgc ttcgtcaccc agcgcgagct gccgcgcatg
     5161 gccctggtgc gcccgcagct gcgcgcgctg gacgtggtgc tgcgggcgcc cggcatgctg
     5221 gcgctgcacc tggggatgaa cgaggtcgaa tcgcccaccc gcgtgcaggt gtggggcgac
     5281 gagctggcgg cctgggacat gggccaggtt gccgcccagt ggctgagcga ttttctaggt
     5341 cggcccggcc tgcggctggc gcgcttcgac cccgaggtga cgcggctggc cagccggcgc
     5401 tggagcggcg acatcgaggc gccgatcgag ttcgccgacg gttttccgct gctgctggcc
     5461 agccaggcct cgctggacga gctgaacgcc cgcctgggcg cggccggcca cccgccggtg
     5521 ggcatggagc gctttcgtcc caacctggtg ctggacggcg tgtcggcgca ggacgaggac
     5581 cgtctggccg agctgcgcat caccaccgcc gagggccggc aggtggtgct gcgcccggtc
     5641 aagccctgcg cgcgctgccc gattccgaac gtcgatccgg ccagcgccgc catccacccc
     5701 gaggtgctgg ccacgctgtc gacctaccgc gccaatccgg tgctggaggg tgccgtcacc
     5761 ttcggcatga actgcgtcat cgtgcaaggc gccggcgcca cgctgcgcgt gggcgacgcc
     5821 gtggcggccg actggcgttt cgaatgatgc taccaataac gtagcttctg gcgcttgcca
     5881 gcaaagcgct ggagcccgat ttgatgccta tccggcgggc gaccctgccg caggggcgcc
     5941 ggtacaatcg ccgcttttcc cgaattccga cgagacctct gccatgagcc tgcaatgcgg
     6001 catcgtgggc ctgcccaacg tgggcaagtc caccctcttc aacgccttga ccaaggccgg
     6061 catcgccgcc gaaaactacc ccttctgcac catcgagccc aacaccggcg tggtcgaggt
     6121 gcccgacccg cgtctggcgc agctggccgc catcgtcaag ccggagcggg tgttgccggc
     6181 catcgtcgag ttcgtcgaca tcgccggtct ggtggccggc gccagccagg gcgaaggcct
     6241 gggcaaccag tttctggccc acatccgcga gaccgacgcc atcgtcaacg tggtgcgctg
     6301 ctttgaagac cccaacgtga tccacgtggc cggcaaggtg gacccgatcg ccgacatcga
     6361 ggtgatccag accgagctgt gtctggccga cctgggcacg gtcgaaaagg cgttgcagcg
     6421 ctacaccaag gccgccaaga gcggcaacga caaggaagcc gccgccatgg tcaagctgct
     6481 gggcccgctg caggcggcgc tgaacgaagc acggccggtg cgcacgctgg cgctgtcgaa
     6541 ggaagagcag gcgctgctca aaccgctgtg cctgatcacc gccaagcccg ccatgttcgt
     6601 cggcaacgtg gccgaggacg gcttcgacaa caacccgctg ctggatcggc tgaacgccta
     6661 cgccgccagc cagaacgcgc cggtggtggc catctgcgcc aagatggaag ccgacatggc
     6721 cgagatgagc gacgaggaca aggccatctt tctggaagaa atgggccaga gcgagccggg
     6781 cctcaaccgc ctgatccgcg ccgccttcaa gctgctgggc ctgcagacct acttcaccgc
     6841 cggcgtcaag gaagtgcgcg cctggaccat ccacatcggc gacaccgcgc cgcaggccgc
     6901 cggcgtgatc cacggcgact tcgagcgcgg cttcatccgc gcccagacca tcgccttcga
     6961 cgactacatc cagtaccagg gcgagcaggg cgccaaggat gccggcaaga tgcgcagcga
     7021 aggcaaggac tacgtcgtca aggatggcga cgtgatgaac ttcctgttca acgtct
//
LOCUS       DAOQTZ010000123         6927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_12043_length_6927_cov_13.273428, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000123 DAOQTZ010000000
VERSION     DAOQTZ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12043_length_6927_cov_13.273428"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PKN51_11900"
     CDS             complement(<1..1442)
                     /locus_tag="PKN51_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNT85844.1"
                     /translation="MQTPHTLPASVAGPPGTLAHDRAGTPTATTQTLTHYQIIRRNGA
                     VVGFEPAKIAVAMMKAFLAVHGTPGAASASVRETVEQLTQTVVRALLRSRPAGGTFHI
                     EDVQDQVELGLMRGGHHEVARAYVLYRERRAQERARQAAGSQPAAPVLHVLQKGELVA
                     LDVPALEALLNQACANLDGAVQSAPILAETLRNLYDGVPVEEVYKAAILAARTLIEKE
                     PDYSFATARLLLHTIAAEVLGEAVDLPTLASRYAGNFPDFIRQGVAGELLDERLLQFD
                     LARLGAALKPERDLQFDYLGLQTLYDRYFLHLNKRRIELPQAFFMRVAMGLALNEIDR
                     EARAIEFYELLSSFDFMSSTPTLFNSGTRRPQLSSCYLTTVPDELEGIYESIKENALL
                     SKHAGGLGNDWTRVRALGSHIKGTNGESQGVVPFLKVANDTAVAVNQGGKRKGAVCAY
                     LETWHLDIEEFLELRKNTGDDRRRTHDMNTA"
     gene            complement(1768..2376)
                     /gene="ampD"
                     /locus_tag="PKN51_11905"
     CDS             complement(1768..2376)
                     /gene="ampD"
                     /locus_tag="PKN51_11905"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNT85845.1"
                     /translation="MKASEPSASAPRRAAWQGGWYRAARALPSPNHGPRPAGSEVDLI
                     VVHSISLPPGKYGGDQVQALFTNTLDWDAHPYFQPLRGLQVSAHFYVRRDGRLWQFVS
                     CDARAWHAGVSCFRGRENCNDYSVGVELEGLEGEPFEPAQYATLARLCRALARRYPIE
                     AIAGHQHIAPGRKHDPGPGFDWRRLQGLLRWSARRFADGALG"
     gene            complement(2363..3901)
                     /locus_tag="PKN51_11910"
     CDS             complement(2363..3901)
                     /locus_tag="PKN51_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT85846.1"
                     /translation="MNASVTMPGAASILVVDDEPDLRTLYELTLLREGHRVSSAASLA
                     EARALLAAQAYDLVVTDMRLTDGLGLELLREMAAAQRRERSIVITAYGSAENAVEALK
                     CGAFDYLTKPIDLKQFRGVVGAALQARSATPADDAAPAREPAPPTPAATPAPAAPAAA
                     ASGPGAVALARLVGESAAMRAVKQRIAKVARSMAPVLVCGESGTGKELVARAIHACSH
                     RSEGPMVPVNCGAIPENLLEAEFFGARKGSYTGATQDRVGFFQAARGGTLFLDEIGEL
                     PLAMQAKLLRAIQERRVRPLGETQEEPVDVRIVSATHKDLAAEVQAGRFRQDLYYRLN
                     VIEIVVPPLRERREDLPALCQTLLGRIAADAGIAVPTLSARQLQDIRTAPLEGNVREL
                     ENLLHRAVALGEAGASGFGELESGTPSPQAATDSIAISGPTVAPPDAPRTPPHTDLPS
                     NLESWLDAREREVLVRALDETGFNRTAAAARLGLNLRQMRYRMARLGITAPSDDEPGP
                     DEGK"
     gene            complement(3924..5663)
                     /locus_tag="PKN51_11915"
     CDS             complement(3924..5663)
                     /locus_tag="PKN51_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNT85847.1"
                     /translation="MSSRRAHEQATWFGTQLPTAPSLPALTLRADADPSPGFARLWSA
                     FMSARVLIALALLGLQLVLHVLARPGSPWLLGTSVAYALFTIAVRVMGEPRGPGRAFD
                     SQWLPTVGVDLLFFSALQLQPTPSPINYTPLLALPVLMAAIMGTRALALGTAALVTLM
                     LLGHAAWAASGSSWANTAELLQAGLTGAGLLVLALLTNQMAMRLAREEALARRNRVEA
                     HVQAMVNAMVIEALPDGVLVIDTEHLVRAANPAARLMLGSDLDVTPPVFSLEDNPAWG
                     QLKRIVGQTFSDAPVDATEVTLHHEDRMSSHLQVRTQRTPSIGDSGVSLCVMFLQDLR
                     ELEARLRTEKLASMGRMSAAVAHEIRNPLAAISQANALLDEDLHEPLQKRLADMVRQN
                     AERLQHIVDDVLDVARVQGQGGEFSPVPALALDAEVEDFCNEWAAQHGQVQRLLLRLG
                     AGDANVQFAPEHLRRVLVNLLDNAARYASRRAGAIQVETLAVRHGPVRLMVWSDGPAM
                     EAAVQRHLFEPFFSSESRSSGLGLYICRELCERHGASIAHERNARAQGGQSHEGNEFF
                     ISFRRAHSGHSTF"
     gene            complement(5663..5890)
                     /locus_tag="PKN51_11920"
     CDS             complement(5663..5890)
                     /locus_tag="PKN51_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HNT85848.1"
                     /translation="MKLLIVLLAVLAAVWLWRRGRRPSAPPPSDRRGRGQALPMVRCD
                     RCGVHLPGADAVRGHRGSYCCVAHRRDAEGA"
     gene            complement(5887..6696)
                     /gene="ccsA"
                     /locus_tag="PKN51_11925"
     CDS             complement(5887..6696)
                     /gene="ccsA"
                     /locus_tag="PKN51_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNT85849.1"
                     /translation="MILASASPVSLGLAAAAALAYAVPALAARALGAAGARRVLLLAW
                     LLHGAQLAWGVLGSEPRFGFAPALSITVWLVLTVYAIESQLYPQMTARWALAGFGTLA
                     ILLALLFPGAHPPATGSPLLPLHWALGIAAYGLLAAAVAHGWLMQRAEDQMRHATQTV
                     DGGVPLLTLERLMFRFLGAGFVLLTLTMLAGALFGEQLYGASYTGWRWDHKRVFTLLS
                     WLTFGTLLAGRWWFGWRGRRAVRVLYIGAALLLLGYVGSRFVLEVLLGRVA"
     gene            6800..>6927
                     /locus_tag="PKN51_11930"
     CDS             6800..>6927
                     /locus_tag="PKN51_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNT85850.1"
                     /translation="MATALTDKLSGLVKKISGQARITEANVQDMLREVRMALLEADV"
BASE COUNT          989 a         2430 c         2514 g          994 t
ORIGIN      
        1 gcggtgttca tgtcgtgcgt gcggcggcgg tcgtcgccgg tgttcttgcg cagctccaga
       61 aactcctcga tgtccagatg ccaggtttcc aggtaggcgc acacagcccc cttgcgcttg
      121 ccgccctggt tgacggccac cgcggtgtcg ttggccactt tcagaaacgg caccaccccc
      181 tgcgattcgc cgttggtgcc cttgatgtgg ctgcccagtg cgcgcacgcg cgtccagtcg
      241 ttgcccaggc cgccggcgtg cttggacagc agggcgtttt ccttgatcga ctcgtagatg
      301 ccctccagct cgtcgggcac cgtggtcagg tagcagctcg acagctgcgg gcgccgcgtg
      361 ccgctgttga acagcgtcgg cgtgctgctc atgaagtcga acgacgacag cagctcgtag
      421 aactcgatgg cgcgcgcctc gcggtcgatc tcgttcagcg ccagacccat ggccacccgc
      481 atgaagaagg cctgcggcaa ctcgatgcgg cgcttgttca ggtgcaggaa gtagcggtcg
      541 tacagcgtct gcaggcccag gtagtcgaac tgcaggtcgc gctcgggctt gagcgccgcg
      601 cccaggcgcg cgaggtcgaa ctgcagcagg cgctcgtcga gcagctcgcc ggccacgccc
      661 tggcggatga agtcggggaa gttgccggcg tagcggctgg ccagcgtcgg caagtccacc
      721 gcctcgccca gcacctcggc ggcgatggtg tgcagcagca ggcgcgccgt ggcgaagctg
      781 tagtcgggct ctttctcgat cagcgtgcgc gcggccagga tggcggcctt gtagacctcc
      841 tcgaccggca cgccgtcgta caggttgcgc agggtttcgg ccaggatggg ggcgctctgc
      901 acggcgccgt ccaggttggc gcaggcctga ttcaaaaggg cctccagcgc aggcacatcc
      961 agcgccacaa gctccccttt ttgcagcacg tgcagcaccg gcgcggccgg ctgactgccg
     1021 gccgcctggc gcgcccgctc ctgcgcgcgg cgttcgcggt agagcacgta ggcgcgcgcc
     1081 acctcgtggt ggccgccgcg catcaggccc agctcgacct ggtcctgcac gtcctcgatg
     1141 tggaaggtgc cgccggccgg gcgtgaacgc agcagggcgc gcaccacggt ctgcgtcagt
     1201 tgctccaccg tttcgcgcac gctggccgaa gccgcgccgg gtgtgccgtg cacggccagg
     1261 aaggccttca tcatggccac cgcgatcttg gcgggctcga acccgaccac ggccccgttg
     1321 cgccggatga tctggtagtg cgtcagcgtc tgcgtggtcg cggtcggtgt gccggcgcgg
     1381 tcgtgcgcga gtgtgcccgg ggggccggcg acgctggccg ggagggtgtg cggtgtctgc
     1441 atggggtcct cggtggatgg gtggcggaat gtcgatgcgc gacgcgctga cgcgccggtc
     1501 tggccggcgc caggcggccg cgacgacgga cactatatct tgtgtttgct aatcgttcaa
     1561 tgcacaaggg gtagtgtgtg cgggcaaacg cgtagcctgg tgccggagca cccgaggccc
     1621 gcagcgggcg cgccgtcagg ctcggcgcgg gtgggcgggc gcgcgccacg caggggcgcg
     1681 gcctgtcgca ggatttgcgc cgccagggcg catggatcaa ggcgcggtgc ccggtcgatg
     1741 cgggcgcggc ggcgccgaac tcgtggatta ccccagggcg ccgtcggcaa agcgcctggc
     1801 gctccagcgc agcaggcctt gcaggcgccg ccagtcgaag cctggcccgg ggtcgtgctt
     1861 gcggccgggc gcgatgtgct ggtgcccggc gatggcctcg atcgggtagc gccgcgccag
     1921 cgcgcggcac agccgggcca gcgtggcgta ctgcgccggc tcgaagggtt cgccttccag
     1981 gccttcgagc tcgacgccca ccgagtagtc gttgcagttc tcgcgcccgc gaaagcacga
     2041 caccccggca tgccaggcgc gcgcgtcgca gctgacgaac tgccacagcc gaccatcgcg
     2101 ccgcacgtag aaatgcgccg acacctgcaa cccccgcaac ggctggaagt agggatgcgc
     2161 gtcccagtcc agcgtgttgg tgaacagggc ctgcacctgg tcgccgccgt acttgccggg
     2221 cggcaggctg atcgagtgca cgacgatcag gtcgacctcg ctgcccgccg ggcgtgggcc
     2281 gtggttgggc gagggcaggg cgcgcgccgc ccggtaccag ccgccctgcc aggcggcgcg
     2341 gcgtggcgcg cttgcgctgg gttcacttgc cttcatcggg gcctggttcg tcgtcgctcg
     2401 gggcggtgat gcccaggcgc gccatgcggt agcgcatctg gcgcaggttc aggcccagcc
     2461 gggccgcggc ggcggtgcgg ttgaagccgg tttcgtcgag cgcgcgcacc agcacctcgc
     2521 gctcgcgcgc gtccagccag ctttccaggt tgctgggcag gtcggtgtgc ggcggcgtcc
     2581 ggggggcgtc gggtggcgcc accgtgggac ccgagatcgc tattgaatcg gtagctgcct
     2641 gcggtgatgg ggtgccggat tcaagctccc cgaacccgga agcgccggct tcgcccaggg
     2701 cgacggcgcg gtgcagcagg ttttccagtt cgcgcacgtt gccctccagc ggggcggtgc
     2761 ggatgtcctg caactggcgt gccgacaacg tgggtacggc gatgccggca tcggcggcga
     2821 tgcgccccag cagggtctgg cacagggccg gcaggtcttc gcgccgctcg cgcaggggcg
     2881 gcaccacgat ttcgatcacg ttcaggcggt agtacaggtc ctgccggaag cgcccggcct
     2941 gcacctcggc cgccaggtcc ttgtgggtgg cgctgacgat gcgtacgtcg accggctctt
     3001 cctgggtttc gcccagcgga cgcacgcgcc gctcctggat ggcgcgcagc agcttggcct
     3061 gcatggccag cggcagctcg ccgatctcgt cgagaaacag cgtgccgccg cgcgccgcct
     3121 gaaagaagcc cacgcggtcc tgcgtggcgc cggtgtagga gcccttgcgg gcgccgaaga
     3181 actcggcctc cagcaggttc tccgggatgg cgccgcagtt caccggcacc atggggccct
     3241 cgctgcggtg gctgcaggcg tggatggcgc gcgccaccag ctccttgccg gtgcccgatt
     3301 cgccgcacac cagcaccggc gccatgctgc gggccacctt ggcgatgcgc tgcttgacgg
     3361 cgcgcatggc ggccgattcc cccaccagcc gcgccagcgc aacggcgccg gggccgctgg
     3421 cggcggcagc gggcgcggcg ggcgccggcg tggccgccgg ggtcggtggg gccggttcgc
     3481 gcgcgggcgc ggcgtcgtcg gccggggtgg cgctccgggc ttgcagcgcc gcgccgacca
     3541 cgccgcggaa ctgtttgagg tcgatcggct tggtcaggta gtcgaaggcg ccgcacttga
     3601 gggcttcgac cgcgttctcg gccgagccgt aggcggtgat gacgatgctg cgttcgcgcc
     3661 gctgggccgc cgccatctcg cgcagcagtt ccagccccag gccgtcggtc aggcgcatgt
     3721 cggtgacgac caggtcgtag gcctgggcgg ccagcagcgc ccgtgcctcg gccagcgacg
     3781 ccgccgaact cacccgatgg ccctcgcgca gcagcgtcag ctcgtacagg gtgcgcaggt
     3841 cgggttcgtc gtccaccacc aggatgctgg cggcgccggg catggtcacg gaagcgttca
     3901 tggctgcgcg cagagctcca gaatcagaaa gtcgagtgtc cgctgtgggc acgccggaag
     3961 ctgatgaaga attcgttgcc ctcgtggctt tgcccgccct gagcgcgcgc gttgcgctcg
     4021 tgggcgatgc tggcgccgtg gcgctcgcac agttcgcggc agatgtacag gcccaggccg
     4081 ctggagcggc tttcggacga gaaaaagggt tcgaacaggt ggcgctgcac cgccgcctcc
     4141 atggccgggc cgtcgctcca gaccatcagc cgtaccgggc cgtgccgcac cgccagcgtc
     4201 tccacctgga tggcgccggc gcggcgcgac gcgtagcgcg cagcgttgtc cagcaggttg
     4261 accagcacgc gccgcaggtg ctcgggcgcg aactgcacgt tggcgtcgcc ggcgcccagc
     4321 cgcagcagca gccgctgcac ctgaccatgc tgggccgccc attcgttgca aaagtcttcc
     4381 acctcggcgt cgagcgccag cgccggcacc gggctgaact cgccgccctg gccctgcacc
     4441 cgggcgacat ccagcacgtc gtccacgatg tgctgcaggc gctcggcgtt ctgccgcacc
     4501 atgtcggcca gacgtttctg caacggttcg tgcagatcct cgtcgagcag ggcgttggcc
     4561 tggctgatgg ccgccagcgg gttgcggatc tcgtgcgcca cggcggccga catgcgaccc
     4621 atcgacgcca gtttctcggt gcgcaggcgc gcttccagct cgcgcaggtc ctgcaggaac
     4681 atcacgcaca ggctgacgcc actgtcgccg atcgatggtg tgcgctgggt gcgcacctgc
     4741 aggtggctgc tcatgcggtc ttcatggtgc agggtcactt cggtggcatc gaccggcgcg
     4801 tcactgaagg tctgaccgac gatgcgcttg agctgtcccc aggcggggtt gtcctccagg
     4861 ctgaacaccg gcggcgtcac gtccaggtcc gaccccagca tcagccgcgc cgccgggttg
     4921 gccgcccgca ccagatgctc ggtgtcgatc accagcacgc cgtcgggcag ggcttcgatc
     4981 accatggcgt tgaccatcgc ctgcacatgg gcctcgaccc ggttgcggcg cgccagcgct
     5041 tcctcgcgtg ccagccgcat ggccatctgg ttggtcagca gcgccagcac cagcaggccg
     5101 gcgccggtca ggcccgcctg caacagctcg gcggtgttgg cccaggacga gccgctggcc
     5161 gcccacgctg cgtggcccag cagcatcagc gtgaccagcg ccgccgtgcc cagcgccagg
     5221 gcgcgcgtgc ccatgatggc ggccatcagc accggcagcg ccagcaaggg cgtgtagttg
     5281 atgggcgacg gggtgggctg caactgcagc gccgagaaaa acagcagatc gacccccacc
     5341 gtgggcagcc actggctgtc gaaggcacgc ccggggccgc gcggctcacc catcacccgc
     5401 acggcgatgg tgaacagcgc gtaggcgacg ctggtgccca gcagccaggg cgagcccggt
     5461 cgtgccagca catgcagcac cagctgcaga cccagcagcg ccagcgcgat cagcacccgg
     5521 gccgacatga aggccgacca cagccgggca aagccgggcg agggatcggc gtcggccctc
     5581 agggtcaggg ccggcagtga cggcgccgtc ggcagctggg tgccgaacca ggtggcctgc
     5641 tcgtgcgcgc ggcgggacga catcaggcgc cttcggcgtc gcggcggtgc gccacgcaac
     5701 agtagctgcc ccggtggccg cgcaccgcgt ccgcccccgg caggtgcacg ccgcagcggt
     5761 cacagcgcac catgggcagg gcctgccccc ggccgcgccg gtcggacggt ggtggggccg
     5821 acggccggcg tccacgccgc cacagccaga cggccgccag cacggccagc agcacgatca
     5881 gcaacttcac gcgacccgcc ccagcagcac ttccagcacg aagcgcgagc cgacgtagcc
     5941 cagcagcagc agcgccgcgc cgatgtacag cacgcgcacc gcacgccgcc cgcgccagcc
     6001 gaaccaccag cgcccggcca gcagcgtgcc gaaggtcagc caggacaaca gggtgaacac
     6061 ccgcttgtgg tcccagcgcc agccggtgta gctggcgccg tagagctgct cgccgaacaa
     6121 ggcgccggcc agcatggtca gcgtcagcaa cacgaagccg gcgcccagaa agcggaacat
     6181 caggcgctcc agcgtcagca gcggcacgcc cccgtcgacc gtctgcgtgg cgtggcgcat
     6241 ctggtcttcg gcgcgctgca tcagccagcc atgggccacg gcggcggcca gcaggccgta
     6301 ggcggcaatg cccagggccc agtgcagcgg cagcaggggc gagccggtgg ccggcggatg
     6361 ggcgcccgga aacagcagcg ccagcaggat cgccagcgtg ccgaagccgg ccagtgccca
     6421 gcgcgcggtc atctgcgggt agagctggct ttcaatggcg tacacggtca gcaccagcca
     6481 cacggtgatg gacagcgccg gtgcaaagcc gaagcgcggt tcgctgccca gcacgcccca
     6541 ggccagttgt gcgccgtgca gcagccaggc cagcagcagc acgcgccgcg cgcctgcggc
     6601 gcccagggcg cgtgcggcca gcgccggcac cgcgtaggcc agggcggcgg cggcggcgag
     6661 ccccaggctg acgggagagg cgctggctag aatcatgggc aaagtttagc ggcttgcccc
     6721 tctcgtccag cgcggacggg ccggccgccg cgggttttca cacgcttttg gcgcgccctg
     6781 tgcggcgcga accgctcgaa tggccaccgc cctcaccgac aagctctccg gcctggtcaa
     6841 gaagatcagt ggccaggccc gcatcaccga agccaatgtg caggacatgc tgcgcgaggt
     6901 gcgcatggcc ctgctcgaag ccgacgt
//
LOCUS       DAOQTZ010000124         6893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_12114_length_6893_cov_13.477333, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000124 DAOQTZ010000000
VERSION     DAOQTZ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12114_length_6893_cov_13.477333"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..759)
                     /locus_tag="PKN51_11935"
     CDS             complement(19..759)
                     /locus_tag="PKN51_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT85851.1"
                     /translation="MTFVNARAHDASAQQPPRTSEVQRLLGNCRPLWGLPQAVLSEMA
                     GQASVARHADGEMLFYEGDPARHCLLVAQGAVALLRHDERGEERLWQCFGTGALVAEA
                     AMFLPGGLHPLSARAQGAVRVWQIPGQALRAACLAYPELALRMLQCLSQCLHQRVNEV
                     DWLTRGNTQERLASYVLAQAMQQGTCIELPCSQRHLAARLGVRAETLSRLLAQWRARG
                     WVQGRGRHWRLCDRGALQRLAPSGAHPF"
     gene            905..2860
                     /locus_tag="PKN51_11940"
     CDS             905..2860
                     /locus_tag="PKN51_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT85852.1"
                     /translation="MSTSLHPALSCRRHPLALAALLLVSHAATAQSTSAQSSTAALPA
                     VTVTATMTEQDARTAPASVTVITAEELAEKNATDLLDAVRGAPGITLQGRQVGGRKTL
                     ALRGLEGKHTLTLIDGRRISASDDVIGHSDYQYGWLPISAIERVEIIRGPMSALYGSE
                     ALGGVINLITKRPKDRWSGSIGVSGATGLSSDAASEGGTSIYAGGPLGERARLSVNAE
                     YAHRNATPDVDDPRYSEIEGRKPRNLGLNAEFDLTAQQRLQAGISDGKEIRRYDDVNS
                     SGTAYRNRYDLDRRQTHLGWVGDFGDTKAQLRAYRSEMSIRNTRTNGVSPTRPQDMQD
                     DVVDGHVTMRRGGHQIAFGGEWRNEELVNAGLKGGRDDVTHKALFVQDEFALTRNLMA
                     TLGLRADHHGIFGSELSPRAYLVWEASPSLVVKGGYGHAFKAPTLKQISPNYVGAEGP
                     HTFMGNANIKPETSNSFEIGADWQVSPAWSLRATAFHTEVKQLITYRLLQQIGIRRIY
                     QYDNVDAARIQGLEAGFTWAITPQLSWSTDATVLHTRDKTTGKRLNDRPTTSVASHLA
                     WRVDGWDLRLGLQHTGSQESYGNKLPAYTLWNASVGRVWKLGGTQSLNVRAGLENMGN
                     LRLAEKSPNFGYAEQGRRVFLSARLDF"
     gene            2918..>6893
                     /gene="cobN"
                     /locus_tag="PKN51_11945"
     CDS             2918..>6893
                     /gene="cobN"
                     /locus_tag="PKN51_11945"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNT85853.1"
                     /translation="MKLMRWLLCAALLLLVGVARAASVLFIATGNVPQGKFHQLAEIA
                     RPHGIEVEVRYLNSLPADVDAGLWRGRDAVFFDSYLQDEVRDRLVRALPGLAAPNAWL
                     YDARPAWGGGLPEAVARRLITYYASGGRQNYEGFFATLAAQLKGGDALAAAPDPVVFP
                     KTGIYHPRWPGLVTGDVHAYLRGQGVDRAAAGHKPVIAIALHQQYIGSMQTAYIDDMV
                     ARVEAGGAVALPFYTPMMGEGGFPKVLQPGGPGTPVLADLLINTQITLNADERRAEFE
                     RMGIPVIQAMTHRRGDEAEWAASQQGIATMDVPFYLAQAEYAGITDIQVASATRKGDE
                     QIMPITAQAAAVAAKALNLIKLQRKPNADKRVAVMFWNYPAGEKNLSASFLNVPRSLQ
                     TTLAAMAASGYRTEVPPDETHLITLLQRLLAPAYRGGELEPLLRDGLAALLPVKDYRA
                     WLAGLPRPTQEALASAWGAPEKSPWVLRQNGVDYFVIPRLELGHITLLPQPGRSGMAP
                     GSKDARAKEKEIYHSTTDLPPHHYLATYLWTRRHNDALVHYGTHGTQEWLPGKERGLS
                     VYDAPLLALGDIPVAYPYIADNIGEATQAKRRGRATIISHQTPPFAPGGLHEALTQMH
                     DLLHQWQAQDEGAVRERMALDLLAAAKKERVIADMGWTEARAKAQFADFVQELHNHLH
                     ELAQTAQPQGLHTLGRAPEELHRLGTVLLMLGSDFWEAAARHAGVSAADLDEALIGPW
                     DRLAQTVPYRLLKKHVVDGEATTDLPEQLQKALQKAAQVYADIGAQNELKGLLTVLDG
                     RYLATSYGGDPIKNPDAYPTGRNLYGFDPSRIPTKQAWEAGKQAAENLLAEHRKLHGR
                     QPKKLTFSLWSVETMRHFGMLEAQALWLLGVEPVWDQGGRVTGVKLVDRERLGRARVD
                     VVLSATGLYRDHFPNVMKQLAQAVLLASRARDEADNPVAANSERIAQRLIAQGADAKA
                     AELAGATRIFASESGRYGAGLDDAALATDTWEGKAEGDKKLAQLYLSKMQFAYGPDEK
                     QWGSAGVAGAAAKGGKAVNLYAEHLSGTEGAVLSRSSNLYGMLTTDDPFQYLGGIALA
                     VRHLDGKAPELYISNLRGAGSGKVEGAAQFLAKELATRQFHPGYIQGLMKEGYAGTLQ
                     VLDATNNFWGWTATAREIVRDDQWQEMVDVYVRDKHNLGLKDWFESKNPHALAQTMER
                     MLEAARQGYWKADAATVRELKERWRDLAQRHGVQSDNAAFERFVGAAQGAAGYGLAPG
                     APGAAAAAAQAQAQAQDQDQDQDQAQAAEPPPQDEAPPQPPPPPISGMRLEQVQEQPR
                     DSAVP"
BASE COUNT         1164 a         2494 c         2317 g          918 t
ORIGIN      
        1 aatctgccac ttcaggcgtc agaacgggtg cgcgcccgat ggtgccaggc gctgcagcgc
       61 accacggtcg cacagccgcc aatggcgccc tctgccctgc acccagccgc gcgcccgcca
      121 ttgcgcaagc aggcggctga gcgtctcggc gcgcacgccc aaccgggccg ccagatggcg
      181 ctggctgcaa ggcagttcga tgcaggtgcc ctgctgcatg gcctgcgcca gcacgtagct
      241 ggccaggcgc tcctgggtgt tgccacgggt cagccaatcg acttcgttga cgcgctggtg
      301 cagacattgg ctcaggcact gcagcatgcg cagcgcaagt tccgggtacg caagacatgc
      361 cgctcgaagc gcctgacccg gaatctgcca gacccgaacc gcgccctgcg cacgcgccga
      421 cagggggtga aggccaccgg gcagaaacat cgccgcttcg gccaccagcg cgccggtgcc
      481 gaagcactgc caaaggcgtt cctcgccgcg ttcgtcatgg cgcagcagcg ccaccgcacc
      541 ttgcgccacc agcaggcagt gccgtgccgg gtcgccttcg tagaacagca tctcgccgtc
      601 ggcgtgccga gcgacgctgg cttgaccggc catttcactc agcaccgcct gcggcaagcc
      661 ccacaagggc cggcagttgc cgagcaagcg ctgcacctcg ctggtgcgcg gaggctgttg
      721 cgccgacgca tcatgggcgc gcgcattgac aaatgtcatt tgattccggg ctgccatgag
      781 aataattacg gatgcgaatc attcgtatcc gcgtttctat cttaatccct tttttttccc
      841 gcccagccca acgcgcgccg acgggccgat gcgtccagcc accgcggtca atccttggac
      901 gcccatgtca acctccctgc atcccgccct gtcgtgccgt cgccatcccc tggctctggc
      961 cgctcttctg ttggtcagcc acgcggccac ggcgcagtcg acctctgccc agtcctccac
     1021 ggctgccctg ccggccgtca ccgtgacggc caccatgacc gagcaggacg cgcgcaccgc
     1081 gccggccagc gtcaccgtca tcacggccga ggagctggcc gagaagaacg ccaccgacct
     1141 gctggacgcc gtgcgcggcg cgcccggcat cacgctgcag gggcgccagg tgggcgggcg
     1201 caagacgctg gcgctacgcg gcctggaggg caagcacacg ctgacgctga tcgacgggcg
     1261 gcgcatcagc gcgagcgacg acgtgatcgg ccattccgac taccagtacg gctggctgcc
     1321 gatctcggcg atcgagcgcg tggagatcat ccgcggcccg atgtcggcgc tgtacggctc
     1381 cgaggccctg ggcggcgtga tcaacctcat caccaagcgg cccaaggacc gctggagcgg
     1441 ctccatcggc gtctcgggcg cgacggggct gtcgagcgac gcggcgagcg agggcggcac
     1501 ctcgatctac gcgggcggcc cgctgggtga gcgcgcgcgc ctgtcggtga acgccgaata
     1561 cgcccaccgc aacgccacgc ccgacgtgga tgacccgcgc tacagcgaga tcgagggccg
     1621 caagccgcgc aacctggggt tgaacgccga gttcgacctg acggcccagc agcgcctgca
     1681 ggccggcatc agcgacggca aggaaattcg ccgctatgac gatgtgaact cgtctggcac
     1741 ggcctaccgc aaccgctacg acctggatcg gcgccagacc catttgggct gggtgggtga
     1801 ctttggcgat accaaggcgc agctgcgcgc ctaccgcagc gagatgagca tcaggaacac
     1861 gcgcaccaac ggcgtgtcgc ccacgcggcc gcaggacatg caggacgacg tggtcgacgg
     1921 tcatgtgacc atgcgccgcg gcggccacca gatcgccttt ggcggcgaat ggcgcaacga
     1981 ggaactggtc aacgccggcc tcaagggcgg gcgcgacgac gtgacgcaca aggcgctgtt
     2041 cgtgcaggac gagttcgcgc tcacgcgcaa cctcatggcc acgctgggcc tgcgcgccga
     2101 ccaccacggc atcttcggct ccgagctcag cccgcgcgcc tacctggtat gggaggccag
     2161 ccccagcctg gtcgtcaagg gcggctatgg ccacgccttc aaggcgccca cgctcaagca
     2221 gatctcgccc aactacgtgg gcgccgaggg gccgcatacc ttcatgggca atgccaacat
     2281 caagccagaa acatcgaact ccttcgagat tggcgccgac tggcaggtca gccccgcctg
     2341 gtcgctgcgc gccacggcct tccataccga ggtcaagcag ctcatcacct accgcctgct
     2401 gcagcagatc ggcatacgcc gcatctacca gtacgacaac gtggacgccg cgcgcatcca
     2461 gggcctggag gcgggcttca cctgggccat cacgccgcag ctgtcctgga gcaccgacgc
     2521 caccgtgctg cacacgcgtg acaagacgac cggcaagcgc ctgaacgacc gccccacgac
     2581 cagcgtggcc tcgcacctgg cctggcgcgt cgacggctgg gatctgcgcc tgggcctgca
     2641 acacaccggc agccaggaaa gctacggcaa caaactgccc gcctacacct tgtggaacgc
     2701 cagcgtgggc cgggtctgga aactgggcgg cacgcaaagt ctcaacgtcc gcgccggcct
     2761 ggagaacatg ggcaatctgc gcctggccga gaagtcgccc aacttcggct acgccgagca
     2821 ggggcggcgc gtgttcctgt cggcgcggct ggatttctga ctatagaaac agtagctgct
     2881 ggcgcgcgcc catagggcgc aggaggccaa tttgactatg aaactcatgc gctggctgct
     2941 gtgcgccgcg ctgctcctgc tggtcggcgt ggcgcgtgcc gcgtcggtgt tgttcatcgc
     3001 cacgggcaat gtgccgcagg gcaagttcca ccagctggcc gagattgcgc gcccccatgg
     3061 catcgaggtc gaggtgcgct acctgaacag cctgccggcc gacgtcgatg ccggcctgtg
     3121 gcgcgggcgt gatgcggtgt tcttcgacag ctatttgcag gacgaggtgc gcgaccgcct
     3181 ggtgcgcgcg ctgcccggcc tggccgcgcc caatgcctgg ctgtacgacg cacgcccggc
     3241 ctggggcggg ggcctgcccg aggccgtggc gcgccgcctc atcacctatt acgcgagcgg
     3301 cgggcgccag aactacgagg gcttcttcgc cacgctggcg gcgcagctca agggcggcga
     3361 cgcgctggcc gcggcgcccg accccgtggt cttccccaag accggcatct accacccgcg
     3421 ctggcccggc ctggtgacgg gcgacgtgca tgcctatctg cgcggacagg gtgtagaccg
     3481 ggcggccgcg gggcacaagc ccgtcatcgc cattgcgctg caccagcagt acataggctc
     3541 gatgcagacg gcctacatcg acgacatggt cgcgcgcgtg gaggccggcg gcgccgtggc
     3601 cctgccgttc tacacgccca tgatgggcga gggggggttc cccaaggtac tgcaacccgg
     3661 cgggcccggc acgcccgtgc tggccgacct gctcatcaac acccagatca cgctcaacgc
     3721 cgacgagcgc cgcgccgagt tcgagcgcat gggcatcccc gtcatccagg ccatgaccca
     3781 ccgccgtggc gacgaggccg agtgggccgc gagccagcag ggcattgcca ccatggacgt
     3841 gcccttctac ctggcccagg ccgagtacgc gggcatcacc gacattcagg tggcgtctgc
     3901 cacgcgcaag ggcgacgagc agatcatgcc catcacggcc caggccgcgg ccgtggccgc
     3961 caaggcgctg aacctgatca agctgcagcg caagcccaac gccgacaagc gcgtggccgt
     4021 gatgttctgg aactaccccg cgggcgagaa gaacctctcg gcctcgttcc tcaacgtgcc
     4081 gcgcagcctg cagaccacgc tggccgcgat ggcggcctcg ggctaccgaa ccgaggtgcc
     4141 gcccgacgag acgcatctga tcaccctgct gcagcgcctg ctggccccgg cctaccgcgg
     4201 cggcgagctt gagccgctct tgcgcgacgg cctggccgcg ctgctgcccg tcaaggacta
     4261 ccgcgcctgg ctcgccggcc tgccgcgccc cacgcaggag gcgctggcga gcgcctgggg
     4321 cgcgcccgag aaatccccct gggtgctgcg ccagaacggc gtggactact tcgtgattcc
     4381 gcgccttgag ctcggccaca tcacgctgct gccccagccc ggccgcagcg gcatggcgcc
     4441 gggcagcaag gacgcgcgcg ccaaggaaaa ggagatctac cactccacca ccgacctgcc
     4501 gccgcaccac tacctggcca cctatctgtg gacgcgccgg cacaacgacg cgctggtgca
     4561 ctacggcacg catggcacgc aggaatggct gccgggcaag gagcgcggcc tgtcggtgta
     4621 cgacgcgccg ctgctggcgc tgggcgacat tcccgtggcc tacccctaca tcgccgacaa
     4681 catcggcgag gccacccagg ccaagcgccg cgggcgcgcc accatcatca gccaccagac
     4741 cccgccgttc gcccccggcg gcctgcacga ggccctgacc cagatgcatg acctgctgca
     4801 ccagtggcag gcgcaggacg agggcgcggt gcgcgagcgc atggcgttgg atctgctggc
     4861 cgcggcgaag aaggagcgcg tgatcgccga catgggctgg accgaggcgc gcgccaaggc
     4921 gcagttcgcc gacttcgtgc aggagctgca caaccatctg cacgagctgg cccagaccgc
     4981 ccagccccag ggcctgcaca ccctgggccg cgcgcccgag gagctgcatc gcctgggcac
     5041 cgtgctgctc atgctgggca gcgacttctg ggaggccgcg gcgcgccacg cgggcgtgag
     5101 cgcggccgac ctggacgagg ccctgatcgg cccctgggac aggctggcgc agaccgtgcc
     5161 ctaccggctc ttgaaaaagc atgtggtcga tggcgaggcc acgaccgacc tgcccgagca
     5221 gctacagaaa gcattgcaga aggcggcgca ggtctatgcc gacatcggcg cgcagaacga
     5281 gctcaagggc ctgctcacgg tgctggacgg gcgctacctg gctacctcct acggcggcga
     5341 cccgatcaag aaccccgacg cctaccccac gggccgcaac ctctacggct tcgacccctc
     5401 gcgcatcccc accaagcagg cctgggaggc cggcaagcag gcggccgaga acctgctggc
     5461 cgagcaccgc aagctgcacg gcaggcagcc gaagaagctc accttcagcc tctggtcggt
     5521 ggagaccatg cggcatttcg gcatgctcga ggcccaggcc ctgtggctgc tcggcgtcga
     5581 gcccgtgtgg gaccagggcg ggcgcgtcac cggcgtgaag ctggtggacc gcgagcggct
     5641 gggccgcgcg cgcgtggacg tggtgctgtc ggccaccggc ctgtaccgcg accatttccc
     5701 caacgtcatg aagcagctgg cccaggcggt gctgctggcg tcccgcgccc gggacgaggc
     5761 cgacaacccc gtggccgcca acagcgagcg catcgcgcaa agactcatcg cccagggcgc
     5821 cgatgccaag gcggcggagc tcgccggcgc cacgcgcatc tttgcctcgg agagcgggcg
     5881 ctacggcgcc gggctggacg acgcggcgct cgccaccgac acctgggagg gcaaggccga
     5941 gggcgacaag aagctcgccc agctgtatct gtccaagatg cagttcgcct atgggccgga
     6001 cgagaagcaa tggggcagcg ccggcgtggc gggggcggca gccaagggcg gcaaggccgt
     6061 caacctctac gccgagcacc tgagcggcac cgagggcgcg gtgctctcgc gcagctccaa
     6121 cctctacggc atgttgacga ccgacgaccc cttccaatac ctgggcggca ttgcgctggc
     6181 cgtgcggcac ctcgacggca aggcgcccga gctctacatc agcaacctgc gcggcgcggg
     6241 cagcggcaag gtcgagggcg cggcgcagtt cctcgccaag gagcttgcca cgcgccagtt
     6301 ccaccccggc tacatccagg gcctgatgaa ggagggctac gcgggcacgc tgcaggtgct
     6361 ggacgccacc aacaacttct ggggctggac ggccaccgcg cgcgagatcg tgcgcgacga
     6421 ccagtggcag gagatggtcg acgtctacgt gcgcgacaag cacaacctcg gcctcaagga
     6481 ctggttcgag tccaagaacc cgcacgcgct ggcgcagacc atggagcgca tgctcgaggc
     6541 cgcgcgccag ggctactgga aggccgacgc ggccaccgtg cgcgaactca aggaacgctg
     6601 gcgcgacctg gcgcagcgcc atggcgtgca gagcgacaac gccgccttcg agcgctttgt
     6661 gggcgccgcg cagggcgccg ctggctatgg ccttgcgccc ggcgcgcccg gcgcggctgc
     6721 ggccgccgcg caggcccagg cccaggccca ggatcaggat caggatcagg atcaggccca
     6781 ggccgccgag cccccgccgc aggacgaagc cccgccgcag ccgcccccgc cgcccatttc
     6841 cggcatgcgc ctggagcagg tgcaggagca gccccgcgac agcgccgtgc ccg
//
LOCUS       DAOQTZ010000125         6777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_12370_length_6777_cov_14.031389, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000125 DAOQTZ010000000
VERSION     DAOQTZ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12370_length_6777_cov_14.031389"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1874
                     /gene="msbA"
                     /locus_tag="PKN51_11950"
     CDS             57..1874
                     /gene="msbA"
                     /locus_tag="PKN51_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNT85854.1"
                     /translation="MAAPPAPAPPASPSAQQPLGRRLARLWTYFREYRLGWLIAALGT
                     LVSALTEASVPALLKPLLDEGFTQGSLPIWVVPLAIVGLFALRGFAHFASQYALARIA
                     NDGMVKLRRQLFDRLLVAELSLFARQSASQLANTLVYEVQTGTTLLVQALLNLARDSF
                     SVVALLAYLLYLNWSLTLIVLVLVPIVALIMKTMSRRLYRYTRLSQTTTDELAYVVEE
                     NVLAHRMVRLHGAQGQQAGRFDTLSRQLRGLSIKATSASAAAMPLAQMAAAVALSVVI
                     GIALHQGQSGAGQRITVGGFVSFIGAMLMLIQPLRRLTDVVGPVTRGLAALERGLDLM
                     AQVQPEAGPAATATSAASRALGELSLREVTVTYHGAAEPVLQQLSLHVRAGEMVALVG
                     PSGAGKTTLINLLPRFVRPSSGQVLLDGQDIADWPLAALRAQFALVSQDVTMFNDSIA
                     ANVALGAPSIDRERVQRCLQDANLADHVATLPRGIDTVLGHNATELSGGQRQRLAIAR
                     ALYRDAPVLLLDEATSALDTESERLVQQAITRAMAGRTTLVIAHRLSTIEHADRIVVM
                     ERGRIVEQGSHAELTAAGGLYARLHGQPSASDAGSPGNS"
     gene            2119..3354
                     /locus_tag="PKN51_11955"
     CDS             2119..3354
                     /locus_tag="PKN51_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT85855.1"
                     /translation="MSPHSALRLHVPEPTGRPGAKTDFSYLSISPAGAVRQPPIDTAA
                     ADTADLATSLVRVLDSEGRAVGPWAPAIHPERLRRGLRAMLKTRLFDARMLLAQRQKK
                     LSFYMQCLGEEAIAVAHAQALREGDMCFPTYRQQGLLLSRDDISLVELICQLMSNERD
                     PIKGRQLPVMYSYKRAGFFSISGNLATQMPQAVGWAMASAIKGDTKIASAWIGDGSTA
                     ESDFHTSLTFAHVYRAPVIINVVNNQWAISTFQSIAGGEGTTFAQRGVGVGIASLRVD
                     GNDFLAVYAASQWAAERARANHGPTLIEWETYRAGPHSTSDDPSKYRPADDWQRFPLG
                     DPIERLKQHLIAIGEWSQAQHEATHKALEAEILAAQKEAESHGSLLDGRVPSAATIFD
                     DVYRDLPEHLRRQRQQMGV"
     gene            3358..4398
                     /locus_tag="PKN51_11960"
     CDS             3358..4398
                     /locus_tag="PKN51_11960"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNT85856.1"
                     /translation="MEQAVQHATATPMTMIAALRSAMDVMMARDDNVIVYGEDVGYFG
                     GVFRVTEGLQAKYGKTRCFDAPISESGIVGTGIGMAAYGLRPVVEIQFADYVYPATDQ
                     IVSELARLRHRSAGDFSAPMVIRMPCGGGIYGGQTHSQSPEAFFTHVCGLRTVMPSNP
                     YDAKGLLISAIESDDPVIFLEPKRLYNGPFDGHHDRPVVPWSKHELGKVPEGHYRVPL
                     TSAAIFRPGKAVTVLTYGTMVWVSECAARETGIDAEIIDLRSIWPLDLDTIVESVRKT
                     GRCVVVHEATRTSGFGAELVALVQEHCFWQLEAPIERVTGWDTPYPHAQEWAYFPGPG
                     RVGEALKRTLEV"
     gene            4402..5700
                     /locus_tag="PKN51_11965"
     CDS             4402..5700
                     /locus_tag="PKN51_11965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNT85857.1"
                     /translation="MGTYVIRVPDIGEGIAEVELVAWHVQPGDAVSEDQHLADVMTDK
                     ATVEVPSPVAGKVLTLGGGVGQVMAVGAELVRLDVEGAGNFKENEASAPAPSAPAAPV
                     SIATEVIAQAPDAAPRAPAAAAARGSVAAPRPVAGRRKEGERPLASPAVRRRALDLGV
                     ELRFVHGSGPAGRIGHQDLDAYVAGQGQAMLTGASPYAERHGEEAVPVIGLRRKIAQK
                     MQEAKRRIPHFSYVEEIDVTELEALRQQLNQRHGATRGKLTLLPFLARAMVCSLRDFP
                     QINARYDDDAGVVTRHAPVHLGVATQTDGGLMVPVLRHAESLDLWAFAAGLTRVTEAA
                     RTGKAAREELSGSTITITSLGPLGGVVTTPVINHPEVAIVGVNRIIERPMLRGGQVVA
                     RQLMNLSSSFDHRVVDGMDAARFIQAMRALLETPALLFVE"
     gene            5710..>6777
                     /locus_tag="PKN51_11970"
     CDS             5710..>6777
                     /locus_tag="PKN51_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT85858.1"
                     /translation="MTKEVTTKLLIIGGGPGGYVAGIRAGQLGIPTMLVEGASLGGTC
                     LNIGCIPSKALIHAAHEFDQARAQATGSPTGIRVQRPQIDIAQTVRWKDGIVQRLTGG
                     VGGLLRRAGVQVLKGWAEIEDGKTVTVKPHQGEPMRVRCEHLLLATGSEPVELPSMPF
                     GGAIWSSTDALSPDLLPRRLVVVGAGYIGLELGMAYRKLGVDVTVVEAAQRVLPSYDE
                     ELTEPVLQALQKQGVVLHLGCSVAGWDAQHGVHVRIDARAEDFALPADRVLVAVGRRP
                     RTAGFGLESLQLDMAGRHIAIDAHGRTSMRNVWAIGDVTGEPMLAHRAMAQGECVAEQ
                     IAGRNRRFEPMAIPAVCFTDPE"
BASE COUNT         1052 a         2450 c         2298 g          977 t
ORIGIN      
        1 cgccgcaggg gctgtcgtgg ggccttcgta gaatccacgc accccgcacc ccgcccatgg
       61 ccgccccacc cgctcccgcc ccgcccgcgt caccgtctgc gcagcagccc ctgggccggc
      121 gactggcacg gctgtggacc tacttccgcg aataccgcct gggctggctg atcgccgccc
      181 tgggcaccct ggtgagcgcg ctgacggaag cctcggtgcc ggccctcctc aagccgctgc
      241 tggacgaggg cttcacccag ggcagcctgc cgatctgggt ggtgcccctg gccatcgtcg
      301 ggctgttcgc cctgcgcggt tttgcccact tcgccagcca gtacgcactg gcgcgcatcg
      361 ccaacgacgg catggtcaag ctgcgccggc agttgttcga ccgcctgctg gtggccgagc
      421 tgtcgctgtt cgcgcgccag agcgccagcc agctcgccaa cacgctggtc tacgaagtgc
      481 agaccggcac caccttgctg gtgcaggcgc tgctgaacct ggcgcgtgac agtttctccg
      541 tcgtcgcgct gctggcctac ctgctgtacc tgaactggtc gctgacgttg atcgtgctgg
      601 tgctggtgcc gatcgtggcg ctgatcatga agaccatgtc gcgccgcttg taccgctaca
      661 cccggctgtc gcaaaccacc accgacgagc tggcctatgt ggtggaagaa aacgtgctgg
      721 cccaccgcat ggtgcgtctg cacggcgcgc aggggcagca ggccggccgc ttcgacaccc
      781 tcagccgcca gctgcgcggg ctgtcgatca aggccaccag cgcctcggcc gccgccatgc
      841 cgctggcgca gatggcggcg gcggtggcgc tgtcggtggt gatcggcatt gccctgcacc
      901 agggccagag cggcgccggg cagcgcatca cggtgggcgg tttcgtgtcc ttcatcggcg
      961 ccatgctgat gctgatccag ccgctgcgcc gcctgaccga cgtggtcggg ccggtcacgc
     1021 gcggactggc ggcgctggag cgtggcctgg acctgatggc ccaggtgcaa cccgaagccg
     1081 gccccgccgc caccgcaacg agcgcggcgt cgcgcgccct tggcgagctg tcactgcgcg
     1141 aggtcacggt gacgtaccac ggtgcggccg agccggtgct gcagcagctc agcctgcacg
     1201 tgcgggccgg cgagatggtg gccctggtgg ggccgtcggg cgccggcaag acgacgctga
     1261 tcaacctgct gccgcgcttc gtgcgaccga gctcggggca ggtgctgctc gatggccagg
     1321 atatcgccga ctggcctttg gcggcgctgc gggcgcagtt tgccctggtc agccaggacg
     1381 tgacgatgtt caacgacagc atcgccgcca acgtggcgct gggcgcgccg tcgatcgacc
     1441 gcgagcgtgt gcagcgctgc ctgcaggacg ccaacctggc cgaccacgtg gccacgctgc
     1501 ccaggggcat cgacaccgtg ctgggccaca acgccaccga actgtcgggc ggccagcgcc
     1561 agcggctggc catcgcgcgc gcgctgtacc gcgacgcgcc ggtgctgctg ctggacgaag
     1621 ccacctcggc gctcgacacc gaaagcgaac ggctggtgca gcaggccatc acacgcgcca
     1681 tggccgggcg caccacgctg gtcatcgcgc accggctgtc caccatcgaa cacgccgacc
     1741 gcatcgtggt gatggagcgc ggccgcatcg tcgagcaagg cagccacgcc gagctgacgg
     1801 ccgctggcgg gctgtatgcg cggctgcacg ggcagccgtc ggcgtcggac gccggcagcc
     1861 cgggtaactc ctgatttcat agctgctggc gcgctccaga taagcgccgg caccccattt
     1921 cgttcatcac cgcacgggct gaaccggcgt cggcggcggc aggttccgtg tgcgaggggc
     1981 tggcgcacag accccacaga ccctacgatg gcccctgcgc caccgcgcac cgatggcaag
     2041 cgttcgtgcg ccgtgatccg gcgccctgtc ttgtccgacg acaaacgcac gacacgatcg
     2101 tgccgatgga gaccgagcat gtcaccacac tcagccctca ggctgcatgt gcccgagccg
     2161 acgggacggc cgggcgccaa gaccgatttt tcctacctca gcatctcacc ggccggtgcg
     2221 gtgcgccaac ccccgatcga caccgccgcg gccgacaccg ccgacctggc caccagcctg
     2281 gtgcgcgtgc tcgacagcga gggccgcgcc gtcggtccct gggcgccagc cattcacccc
     2341 gagcgcctgc gccgcggcct gcgggccatg ctgaagacgc gcctgttcga cgcccgcatg
     2401 ttgctggcgc agcgccagaa aaagctgtcg ttctacatgc agtgtctggg cgaggaagcc
     2461 atcgccgtgg cccatgcaca ggcgctgcgc gaaggcgaca tgtgctttcc cacctaccgc
     2521 cagcagggcc tgctgctgtc gcgcgacgac atctcgctgg tggagctgat ctgccagctg
     2581 atgagcaacg agcgcgaccc gatcaagggg cgccagctgc cggtgatgta ttcctacaag
     2641 cgcgccggct tcttttcgat ctccggcaac ctggccacgc agatgccgca ggccgtgggc
     2701 tgggccatgg cctcggcgat caagggcgac accaagattg cctcggcctg gatcggcgac
     2761 ggctccaccg ccgagagcga ctttcacacc tcgctcacct tcgcccatgt gtaccgcgcg
     2821 ccggtgatca tcaacgtggt caacaaccag tgggccatct ccaccttcca gtcgattgcc
     2881 ggcggcgagg gcaccacctt cgcccagcgc ggcgtgggtg tgggcatcgc ctcgctgcgc
     2941 gtcgacggca acgacttcct ggccgtgtac gcggcctcgc aatgggcggc cgagcgtgcg
     3001 cgcgccaacc atggcccgac gctgatcgag tgggaaacct accgcgccgg cccgcattcc
     3061 acctccgacg acccgtccaa gtaccgcccg gccgacgact ggcagcgctt tccgctgggt
     3121 gatccgatcg aacgcctgaa gcagcacctg atcgccatcg gcgaatggag ccaggcccag
     3181 cacgaagcca cccacaaggc gctggaagcc gagatcctgg cggcacagaa ggaggccgaa
     3241 agccacggca gcctgctcga cggccgcgtg cccagcgccg cgaccatctt cgacgacgtg
     3301 taccgggacc tgcccgagca cctgcgccgg cagcgccagc agatgggggt atgagccatg
     3361 gagcaagctg tccagcacgc caccgccacc cccatgacca tgatcgccgc gctgcgctcg
     3421 gcaatggacg tgatgatggc ccgcgacgac aacgtcatcg tctatggcga ggacgtgggg
     3481 tacttcggcg gcgtgttccg cgtcaccgaa ggcctgcagg ccaagtacgg caagacgcgc
     3541 tgcttcgacg cgccgatctc cgaatccggc atcgtcggca ccggcatcgg catggccgcc
     3601 tacggcctgc gcccggtggt ggagatccag ttcgccgatt acgtctaccc ggccaccgac
     3661 cagatcgtgt cggagctggc gcggctgcgc caccgctcgg ccggcgactt cagcgcgccg
     3721 atggtgatcc gcatgccctg cggcggcggc atctacggcg gacagacgca cagccagagc
     3781 cccgaggcct ttttcaccca cgtctgcggc ctgcgcaccg tcatgcccag caacccgtac
     3841 gacgccaagg gcctgctgat ctcggccatc gaaagcgacg acccggtgat cttcctggag
     3901 cccaagcgcc tgtacaacgg ccccttcgac ggccaccacg accggccggt ggtgccctgg
     3961 tccaagcatg agctgggcaa ggtgcccgaa ggccactacc gcgtgccgct caccagcgcc
     4021 gccatcttcc gccccggcaa ggcggtgacg gtgctgacct acggcaccat ggtctgggtc
     4081 agtgagtgcg ccgcgcgcga aaccggcatc gacgccgaga tcatcgacct gcgctcgatc
     4141 tggccgctgg acctggacac catcgtcgaa tcggtccgca agaccggccg ctgcgtggtg
     4201 gtgcatgagg ccacgcgcac cagcggcttc ggcgccgaac tggtggcact ggtgcaggag
     4261 cactgcttct ggcaactgga agccccgatc gaacgcgtca ccggctggga cacgccctac
     4321 ccgcacgcgc aggaatgggc gtacttccct ggtccgggcc gcgtcggcga ggccctcaag
     4381 cgcacgctgg aggtctgaac catgggtacc tacgtgatcc gcgtgcccga catcggcgag
     4441 ggcattgccg aggtggaact ggtggcctgg catgtgcagc cgggcgatgc cgtgagcgaa
     4501 gaccagcacc tggccgacgt gatgaccgac aaggccacgg tggaagtgcc ctccccggtg
     4561 gcgggcaagg tgctgacgct gggcggtggc gtcggccagg tcatggcggt cggcgccgag
     4621 ctggtgcgcc tggacgtcga aggcgctggc aacttcaaag aaaatgaggc ttcagcgccc
     4681 gccccatctg cgccggcagc tcctgtttca atagcaaccg aggtgatcgc ccaagccccg
     4741 gacgcagcac cgcgcgcacc cgcggccgcc gcagcgcgcg gctcggtggc cgcgccgcgc
     4801 cctgtcgccg gccgccgcaa ggagggcgag cgcccgctcg cctcgcctgc ggtgcgccgg
     4861 cgtgcgctcg acctgggggt ggagctgcgc ttcgtgcacg gcagcggccc ggccggtcgc
     4921 atcgggcacc aggacctgga cgcttacgtc gccggacagg gtcaggccat gctgacgggg
     4981 gcgtcgccct acgccgagcg ccatggggaa gaagccgtcc ccgtcatcgg tctgcgccgc
     5041 aagatcgcgc agaagatgca ggaggccaag cgccgcatcc cgcacttcag ctatgtcgag
     5101 gaaatcgacg tcacggaact ggaggcgctg cgccagcagc tcaaccagcg ccacggcgcc
     5161 acccgcggca agctgaccct gctgccgttt ctggcgcgcg ccatggtgtg cagcttgcgc
     5221 gacttcccgc agatcaacgc ccgctacgac gacgacgccg gcgtggtcac gcgccatgcg
     5281 ccggtgcatc tgggcgtggc cacgcagacc gatggcggcc tgatggtgcc ggtgctgcgc
     5341 catgccgaga gcctggacct gtgggccttt gccgccggcc tgacccgcgt caccgaggcg
     5401 gcccgcaccg gcaaagccgc gcgcgaggag ctgagcggct cgaccatcac catcaccagc
     5461 ctggggccgc tgggcggcgt ggtcaccacg cccgtcatca accaccccga ggtggccatc
     5521 gtcggtgtca accgcatcat cgagcgaccc atgctgcgtg gcggtcaggt ggtggcgcgc
     5581 cagctgatga acctgtcgtc ctcgttcgac caccgcgtgg tcgacggcat ggacgcggcg
     5641 cgcttcatcc aggccatgcg cgccctgctc gaaaccccgg ccctgctgtt cgtggagtag
     5701 gcaccccaaa tgaccaagga agtcacgacc aagctgctca tcatcggcgg cggcccaggc
     5761 ggctacgtcg ccggcatacg cgccggccag ctgggcatcc ccaccatgct ggtcgaaggc
     5821 gccagcctgg gcggcacgtg tctcaacatc ggctgcatcc cgtccaaggc gctgatccac
     5881 gcggcccacg agttcgacca ggcgcgcgcg caggcaaccg gctcgcccac cggcatccgg
     5941 gtgcagcgcc cgcagatcga catcgcgcag accgtgcgct ggaaggacgg catcgtgcag
     6001 cgcctgacgg gcggggtcgg cggcctgctg cgcagggccg gcgtgcaggt gctcaagggc
     6061 tgggccgaga tcgaagacgg caagaccgtg accgtcaaac cgcaccaggg cgagcccatg
     6121 cgcgtgcgct gcgagcacct gctgctggcc accggctccg aaccggtgga gctgcccagc
     6181 atgccgttcg gcggcgccat ctggtcatcc accgacgcgc tctcgcccga tctgctgccc
     6241 cggcgcctgg tggtggtcgg cgccggctac atcggcctgg agctgggcat ggcctaccgc
     6301 aagctcggcg tggacgtgac ggtggtcgaa gccgcccagc gcgtgctgcc cagctacgac
     6361 gaggaactga ccgagccggt gctgcaagcg ctgcagaagc agggcgtggt gctgcacctg
     6421 ggatgcagcg tggccggctg ggacgcccag cacggtgtgc acgtgcgcat cgacgcccgc
     6481 gccgaggact ttgcgctgcc ggccgaccgc gtgctggtgg ccgtgggtcg gcgcccgcgc
     6541 accgcgggct ttggcctgga gtcgctgcag ctggacatgg ccgggcgcca catcgccatc
     6601 gacgcgcacg gccgcacctc gatgcgcaac gtctgggcca tcggcgacgt caccggcgag
     6661 cccatgctgg cgcaccgcgc catggcccag ggcgaatgcg tggccgagca gatcgccggc
     6721 aggaaccggc gcttcgagcc catggccatt cccgccgtgt gcttcaccga ccccgag
//
LOCUS       DAOQTZ010000126         6701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_12562_length_6701_cov_12.457117, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000126 DAOQTZ010000000
VERSION     DAOQTZ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12562_length_6701_cov_12.457117"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="PKN51_11975"
     CDS             <1..105
                     /locus_tag="PKN51_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HNT85859.1"
                     /translation="HEPMAWDFVMNFARDNGLQVTEACLLPDLPASDS"
     gene            206..910
                     /locus_tag="PKN51_11980"
     CDS             206..910
                     /locus_tag="PKN51_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT85860.1"
                     /translation="MNADKVAILTAAGSGMGAACARRLAADGFRVAILSSSGKGQALA
                     EQLGGWGVTGSNQSPDDLARLVEGALARWGRVDVLVNSAGHGPKGPILDISDADWQRG
                     MDTYLLNVIRPTRLVVPAMRAQGGGAIVNISSAWAQEPAEAFPTSAVFRAGLAAFAKL
                     FADQHAAAGIRMNNVLPGWIDSLPEQQARRQTVPMQRYGTADEVAATVAFLCSAGAAY
                     ITGQNLRVDGGLMRSI"
     gene            complement(935..1357)
                     /locus_tag="PKN51_11985"
     CDS             complement(935..1357)
                     /locus_tag="PKN51_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85861.1"
                     /translation="MSAFIESLSHDPVERAIAARVSALLANRGLPASRREALIRQAQR
                     ELVAHRTQAPSQERVQAIATAARLPAGYVARSVQVVAARVLVAAVHPRRGLVWIDVGA
                     APQGVADSGPRPIVRPAAAGRAAAARDPFETRRRELRR"
     gene            complement(1693..1998)
                     /gene="rpsT"
                     /locus_tag="PKN51_11990"
     CDS             complement(1693..1998)
                     /gene="rpsT"
                     /locus_tag="PKN51_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNT85862.1"
                     /translation="MAAGKPKKKNPRLASGRKRARQNVKLNAANTSLRSKYRTAVKNV
                     EKAVLAGDKEKASSAFALMQSVVDSVADKGIFHKNKAARDKSRLSAKVKALALQAAA"
     gene            2131..3684
                     /gene="murJ"
                     /locus_tag="PKN51_11995"
     CDS             2131..3684
                     /gene="murJ"
                     /locus_tag="PKN51_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNT85863.1"
                     /translation="MSLFKAASTVALLTLLSRITGLVRDLLMASAFGVSGLTDAFNVA
                     FRIPNLLRRLFAEGAFSQAFVPVLAASKARDGEAATRRLIDQVATALAWALLATCVVG
                     VLAAPLLVLLLASGLDRPSFDAGVQMTRWMFPYIACMSLVALSAGILNTWKHFVVPAA
                     TPVLLNLAMIAAAWWGAPWFARLGIEPIYAMAAGVMLGGLLQLAVQVPALKRRGLLPR
                     IGLRPARLRAAWGDAGVRQVLRLMLPALLGVGVAQLSLLINTQIASYLAAGSVTWVWY
                     ADRLMEFPTALLGVALGVVLTPQLAAARASGDEARYSQMLDWGLRLVLLLALPCAAAL
                     LVFGTPLIATLFHHGRFAAEDVQRVALALAGYGVGLLGLVAVKVLAPGYYASLDIRTP
                     VKIAVVVLVLTQILNAIFVPLLQHAALTLSIGLGALVNAGWLLAGLIRRGSFRPTRGW
                     GVFGAQVLGATLLMSAFLRWGAQHFDWLGLAALPRAGLLAGCIVAAALIYFGALAASG
                     VRLRQLLRR"
     gene            3741..4580
                     /locus_tag="PKN51_12000"
     CDS             3741..4580
                     /locus_tag="PKN51_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNT85864.1"
                     /translation="MRVDLSVPTPLAYFAALVQRDEGLPLLEAAACLAQDADPGLDVQ
                     QVLDEVDRLAGRLQRQVARGAGVIERLRGLNRFFFEELGFGGNLNDYHHPDNSYLHRV
                     LHTRRGIPISLAVLWLELAQGLGLAAQGVGFPGHFLMQVELPEGLVVLDPFTGQSLSL
                     VELRARLDEWQPQPQQALPLAMHLRPASRRQILARMLRNLQQIHRTQRDWTRLVQVQD
                     RLVTLLPEAWDEYRERGLALAELGQTERAQADLSLYLQRARDARDRAQVAERLDALPR
                     SRP"
     gene            complement(4647..6242)
                     /gene="ahpF"
                     /locus_tag="PKN51_12005"
     CDS             complement(4647..6242)
                     /gene="ahpF"
                     /locus_tag="PKN51_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNT85865.1"
                     /translation="MLDQDIKQQLAAYLERVQEPFEMVATLGTDAKSAELRELLNEIA
                     SLRADKITLRTDGQDKRVPSFTLARPGSGTQLRFAAIPMGHEFTSLILALLWTGGHPP
                     KVEPDVLEQIKALDVEQEFEVYMSLSCHNCPDVVQALSLISIYNPRIKTTIIDGGMFQ
                     QEIEAREIMGVPAVFSGGQMFHSGRISLEEILQKLDTGAARRDAAKLNAKAPFDVLVV
                     GGGPAGAAAAIYAARKGIRTGVVAERFGGQVNDTMEIANYPGIPETNGPAYAAALEQH
                     VRQYEVDIMNAQRVAALVPAAQPGGLITVKLDNGGQLQSRSVILATGARWRNVNVPGE
                     QEYKTKGVAYCPHCDGPLFKGKDVAVIGGGNSGVEAAIDLAGVVRHVTVLEFLPEMKA
                     DAVLIRKLRSLPNVSIHTNAQTTEITGANGRVNGLNFKHRADGSDARVELEGVFVQIG
                     LVPNTEWLGDTVERSRFGEIVVDARGATNVPGVFGAGDCTTVPYKQIVVAAGAGSTAA
                     LSAFDYLIRTPAPAPEGSQAVTA"
     gene            complement(6492..>6701)
                     /locus_tag="PKN51_12010"
     CDS             complement(6492..>6701)
                     /locus_tag="PKN51_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNT85866.1"
                     /translation="LRGTFVIDPSGTIKTMEVHSNEIARDVSETVRKLKAARYTAAHP
                     DEVCPAKWKEGEKTLKPSIDLVGKI"
BASE COUNT          875 a         2207 c         2467 g         1152 t
ORIGIN      
        1 cacgagccga tggcctggga cttcgtgatg aacttcgcgc gcgacaatgg cctgcaggtc
       61 accgaagcct gcctgctgcc cgacctaccg gccagcgatt cttgatcaaa tcggcttgca
      121 gcgcccgact ggcgtgcgcc agcagctatg ctaatgatag ttatctggcg acgccgacag
      181 ccgccgccgt gaaggagaca tgcccatgaa tgccgacaag gtcgccatcc tcaccgctgc
      241 cggcagcggc atgggcgccg cctgcgcgcg ccggctggcc gccgacggct ttcgcgtcgc
      301 catcctgtcg tcgtcgggca aggggcaggc gctggcggag cagctgggtg gctggggtgt
      361 caccggctcc aaccagtcgc cggatgactt ggcgcggctg gtggagggcg cgctggcccg
      421 ctggggccgc gtcgacgtgc tggtcaacag cgccggccac ggccccaagg gcccgatcct
      481 ggacatcagc gacgccgact ggcagcgcgg catggacacc tacctgctga acgtgatccg
      541 tcccacccgg ctggtggtgc cggccatgcg ggcgcagggc ggcggtgcca tcgtcaacat
      601 ctccagcgcc tgggcccagg agccggccga agcgtttccg acctcggccg tgtttcgcgc
      661 cgggctggcg gcctttgcca aactgtttgc cgaccagcac gcggccgccg gcatccgcat
      721 gaacaacgtg ctgcccggct ggatcgacag cctgccggag cagcaggcgc gccgccagac
      781 ggtgcccatg cagcgctacg gcacggccga cgaggtggcc gccaccgtgg cctttctgtg
      841 cagcgccggc gcggcctaca tcacggggca gaacctgcgc gtcgatggcg ggctgatgcg
      901 ctcgatctga gcgcatgccg cgcccgtctg cgcctcagcg gcgcagttcg cgccggcggg
      961 tctcgaacgg gtcgcgcgcg gctgcggcac ggccggctgc ggccgggcgg acgatggggc
     1021 gcgggccgct gtcggccacg ccctgcggcg ccgcgccgac gtcgatccac accagaccgc
     1081 ggcgcggatg gacggccgcc accagcaccc gcgccgccac cacctgcacc gagcgcgcaa
     1141 cgtagcccgc aggcaggcgg gcggcggtgg cgatggcctg cacgcgctcc tggctgggcg
     1201 cctgcgtgcg gtgcgccacc agctcgcgct gagcctggcg gatgagggct tcgcgccgac
     1261 tggctggcag gccgcggttg gccagcaggg cactgacacg cgccgcgatg gcgcgctcga
     1321 ccgggtcgtg ggagagggat tcaatgaacg cggacatgaa tgttccttag ctgaacgatt
     1381 gccgcatgcg cccggagcag ggacgtgcgc atcccttgcg cctgcagcag gaccctcctg
     1441 ctgggcgtgc ggccttcgtg ccaaggaaaa attcagtgcg tcatggggga agaggcggga
     1501 gcagtgcttc tcaacgcatg ggcccgccct gtggtggaca agcagcccgc gcggtgaatg
     1561 cgcgtcggcc ttggcaacaa aaaaccgccc gaaggcggtg atttgctttt tgccgacagc
     1621 gcacccgata caaacggcgg cggggtggat gctgccatcc gccccgaccg ggcggtttgc
     1681 ctgagggggc cgtcaggcgg cggcttgcag ggccagggcc ttgaccttgg ccgacaggcg
     1741 gctcttgtcg cgagcggcct tgttcttgtg gaagatgccc ttgtcggcga ccgagtcgac
     1801 caccgattgc atcagcgcaa aggcgctgct ggctttttcc ttgtcgccgg cgaggaccgc
     1861 cttctcgacg ttcttcaccg cggtgcggta tttggagcgc agcgaggtgt tcgcggcgtt
     1921 gagcttgacg ttctggcggg cgcgtttgcg acccgacgcc aggcgggggt tctttttctt
     1981 gggctttcca gcggccatga aagtgttcct ttggggtttg tggatgttgt cggcaaaacc
     2041 gggcattgta ccagtgcgca gcggcctgcg ccatgtgcgg gcggcagggc gggtgcggcg
     2101 actacactgg cgcccgatcg tgagtccacc gtgagcctgt tcaaagccgc atccaccgtt
     2161 gccctgctga ccctgctgtc gcgcatcacc ggcctggtgc gcgatctgct gatggcgtcg
     2221 gccttcggcg tcagcggtct gacggacgcc ttcaacgtgg ccttccgcat ccccaacctg
     2281 ctgcgccggc tgtttgccga aggggcgttc agccaggcct tcgtgccggt gctggccgcc
     2341 agcaaggcgc gcgacggcga ggcggcgacc cggcggttga tcgaccaggt ggcgacggcg
     2401 ctggcctggg cgctgctggc gacctgcgtg gtcggcgtgc tggccgcgcc gctgctggtg
     2461 ctgctgctgg ccagcgggct ggaccgcccc agcttcgacg ccggcgtgca gatgacgcgc
     2521 tggatgttcc cctacatcgc ctgcatgtcg ctggtggcgc tgtcggccgg gattctgaac
     2581 acctggaagc atttcgtcgt gccggcggcc acgccggtgc tgctgaacct ggccatgatc
     2641 gccgccgcgt ggtggggcgc gccctggttc gcgcgcctgg gcatcgagcc gatctatgcc
     2701 atggccgcgg gcgtgatgct gggcggtctg ctgcaactgg cggtgcaggt gccggcactg
     2761 aagcggcgcg gcctgctgcc acgcatcggc ctgcggccgg cgcgcctgcg cgcggcctgg
     2821 ggcgacgccg gggtgcgcca ggtgctgcgg ctgatgctgc cggcgctgct gggggtgggg
     2881 gtggcgcagc tgtcgctgct gatcaacacg cagatcgcct cgtacctggc ggccggcagc
     2941 gtcacctggg tgtggtatgc cgaccggctg atggagtttc cgaccgcctt gctgggcgtg
     3001 gcgctggggg tggtgctgac gccgcagctg gcggcggcgc gcgccagcgg cgacgaagcg
     3061 cgctactccc agatgctgga ctggggcctg cgcctggtgc tgctgctggc cttgccctgc
     3121 gcggcggcgc tgctggtgtt cggcacgccg ctgatcgcca cgctgtttca ccacggtcgg
     3181 tttgccgccg aggacgtgca gcgggtggcg ctggcgctgg ccggctatgg cgtcggtctg
     3241 ctggggctgg tggcggtgaa ggtgctggcg ccgggctact acgccagcct ggacatccgc
     3301 acgccggtga agatcgcggt ggtggtgctg gtgttgacgc agatcctgaa cgcgatcttc
     3361 gtgccgctgc tgcagcatgc ggcgctgacc ctgtcgatcg gcctgggcgc gctggtcaat
     3421 gccggctggc tgctggccgg gctgatccgc cgcggcagct ttcggccgac gcgcggctgg
     3481 ggcgtgtttg gcgcccaggt gctgggcgcg acgctgctga tgagcgcctt tttgcggtgg
     3541 ggcgcgcagc atttcgactg gctgggcctg gcggcgctgc cgcgagccgg cctgctggcg
     3601 ggctgcatcg tggcggcggc gctgatctac tttggcgcgc tggcggccag cggggtgcgc
     3661 ctgcggcagt tgttgcggcg ctgatgcgcg cttgatggac ggcattgacg cggcaggccg
     3721 cgcgccctta caagagcgtc atgcgcgttg atctgtccgt tcccacgccg ctggcgtact
     3781 tcgccgcgct ggtgcagcgc gacgagggcc tgccgctgct ggaggccgcg gcctgtctgg
     3841 cgcaggacgc cgatcccggg ctcgacgtgc agcaggtgct tgacgaggtg gatcggctgg
     3901 ccgggcgcct gcaacggcag gtggcgcgcg gcgccggggt gatcgagcgc ctgcgcgggc
     3961 tgaaccgctt cttcttcgag gagctggggt tcggcggcaa cctcaacgac taccaccacc
     4021 cggacaacag ctacctgcac cgggtgctgc acacgcggcg cggcatcccg atctcgctgg
     4081 ccgtgctgtg gctggagctg gcccagggcc tggggctggc ggcgcagggg gtgggctttc
     4141 cggggcattt tctgatgcag gtggagctgc ccgaagggct ggtggtgctg gatccgttca
     4201 ccggccagtc gctgagcctg gtggaactgc gcgcccgtct tgacgaatgg cagccgcagc
     4261 cgcagcaggc gctgccgctg gccatgcatc tgcgcccggc cagccggcgc cagatcctgg
     4321 cgcgcatgct gcgcaacctg cagcagatcc accgcacgca gcgcgactgg acgcgcctgg
     4381 tgcaggtgca ggatcggctg gtgacgttgc tgcccgaagc ctgggacgaa taccgcgagc
     4441 gcgggctggc gctggccgag ctgggtcaga ccgagcgcgc gcaggccgac ctgagcttgt
     4501 atctgcagcg tgcccgcgat gcgcgcgacc gcgcgcaggt ggccgagcgg ctcgacgcgc
     4561 tgccgcgctc gcggccatga gccgggcgtg ccggcacgaa aaagcccgcg ggacgccgcg
     4621 ggcttgaacg tcgtgccggc cggccgtcag gcggtcacgg cttgcgaccc ttcgggcgcg
     4681 ggcgccgggg tacggatcag gtagtcgaag gcgctcagcg cggcggtgga gccggcgccg
     4741 gcggccacca cgatctgctt gtagggcacg gtggtgcagt cgccggcacc gaacacgccg
     4801 ggcacgttgg tggcgccacg cgcgtcgacc acgatttcgc cgaaccggct gcgctcgacg
     4861 gtgtcgccca gccactcggt gttgggcacc aggccgatct gcacgaacac gccttccagc
     4921 tccacccggg catcgctgcc gtcggcgcgg tgcttgaagt tcaggccgtt gacgcggccg
     4981 ttcgcgccgg tgatttcggt ggtctgggcg ttggtgtgga tgctgacgtt gggcaggctc
     5041 ctgagcttgc ggatcagcac cgcgtcggcc ttcatctcgg gcaggaactc cagcaccgtg
     5101 acatggcgca ccacgcccgc gaggtcgatg gcggcttcga cgccggagtt gccgccgccg
     5161 atcaccgcca cgtccttgcc cttgaacaac gggccgtcgc agtgcgggca gtaggccacg
     5221 cccttggtct tgtattcctg ctcgcccggc acgttcacgt tgcgccagcg cgcgccggtg
     5281 gccaggatga cgctgcggct ttgcagctgg ccgccgttgt cgagtttcac cgtgatcagg
     5341 ccacccggct gcgccgccgg taccagcgcg gccacgcgct gcgcgttcat gatgtcgact
     5401 tcgtactggc gcacatgctg ctccagcgcg gcggcgtagg cggggccgtt ggtttcgggg
     5461 atgccggggt agttggcgat ttccatggtg tcgttgacct gaccgccaaa gcgttcggcc
     5521 accacgccgg tgcggatgcc cttgcgcgcg gcgtagatgg ccgccgccgc accggccggg
     5581 ccgccaccga ccaccagcac gtcgaacggc gccttggcgt tgagcttggc agcgtcacgc
     5641 cgggcggcac cggtgtccag cttctgcagg atctcctcca gcgagatgcg gccgctgtgg
     5701 aacatctggc cgcccgaaaa cacggcgggc acgcccatga tctcgcgcgc ctcgatctcc
     5761 tgctggaaca tgccgccatc gatgatggtg gtcttgatgc gcgggttgta gatgctgatg
     5821 agcgagagcg cctgcaccac gtccgggcag ttgtggcagg acaggctcat gtagacctcg
     5881 aactcctgct ccacgtccag tgccttgatc tgctccagca cgtccggctc caccttgggc
     5941 gggtggccgc cggtccacag cagcgccagg atcagcgagg tgaattcatg ccccatcggg
     6001 atggcggcaa agcgcagctg cgtgccgctg cccggccgcg ccagggtgaa cgacggcacg
     6061 cgcttgtcct gcccgtcggt gcgcagcgtg atcttgtcgg cgcgcaggct ggcgatctcg
     6121 ttgagcagct cgcgcagctc ggccgacttg gcgtcggtgc ccagggtcgc caccatctcg
     6181 aacggctctt gcacgcgctc gaggtaggcc gcgagttgtt gcttgatgtc ttggtccaac
     6241 atggtgatta ctcctgatgt gatagctgtt tgcgcttgat ggataagcgc tagaggcata
     6301 tttgatcaat aaaaaaccga acggcagggc aggcatgccc tgtcgccgtt cggtcgcagg
     6361 cggtcaggcg gcgcagcgag cggaccgagt gcaaggcgga cggcgcgcag caaccggaac
     6421 gtatttgcgg tacgtgagga ttgcgagcac cggccaacgc ggcaatcggt tcgcgcagta
     6481 gccgcgcgcg ctcagatctt gccgaccagg tcgatcgacg gcttcagcgt cttctcgcct
     6541 tccttccact tggccgggca gacctcgtcc gggtgggcgg cggtgtagcg cgcggccttg
     6601 agcttgcgca ccgtctcgct gacgtcgcgg gcgatttcgt tggagtgcac ttccatggtc
     6661 ttgatggtgc cgctggggtc gatcacgaag gtgccgcgca g
//
LOCUS       DAOQTZ010000127         6577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_12879_length_6577_cov_15.490800, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000127 DAOQTZ010000000
VERSION     DAOQTZ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12879_length_6577_cov_15.490800"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKN51_12015"
     CDS             complement(<1..555)
                     /locus_tag="PKN51_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT85867.1"
                     /translation="MDLAFTPDELKFRDEIRAWVKDALPKALADKVHQAQRLTRDDMQ
                     SWARILGKKGWLGYGWPKQFGGPGWTAVQKHLFEEELAMAGAPRIVPFGPVMVAPVIM
                     AYGSPEQQKRFLPGIASGEVWWSQGYSEPGSGSDLASVRTKAERRGDKYIVNGQKTWT
                     TLGQHGEWMFNLVRTSNEGKPQTGI"
     gene            complement(571..1272)
                     /locus_tag="PKN51_12020"
     CDS             complement(571..1272)
                     /locus_tag="PKN51_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNT85868.1"
                     /translation="MNLRPLTPTELRVLGTLMEKARTVPDTYPLSLNAVVSGCNQKTS
                     REPVMNLSEAEAQAALEALKALHLAIESHGSRVARWEHNLQRALTVPEQSAVLLGLLM
                     LRGPQTAAELRLNAERWYRFADISSVEGFLHELAERPADKGGALTLLLARAPGEREPR
                     WTHLLGGPPAIEKEAAGAHPESAAGRFDPTTERLERLEAELAALQATVARLCAELGLS
                     PAGRDAGTDRATTAG"
     gene            1297..1878
                     /gene="purN"
                     /locus_tag="PKN51_12025"
     CDS             1297..1878
                     /gene="purN"
                     /locus_tag="PKN51_12025"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNT85869.1"
                     /translation="MKHIVILISGTGSNMAAIVRAAEREDWAARLGARVAAVISNRPD
                     ARGLQTAQEAGIATAVVDHRQHADRAAFDAALRAEIDRHQPALVVLAGFMRILTPGFV
                     AHYAGRIVNIHPSLLPAFPGLHTHQRAIDMRCRVAGATVHQVTAELDHGAILAQAAVP
                     VLPGDTADTLAARVLTQEHLIYPRAIAELLSKG"
     gene            2080..3345
                     /locus_tag="PKN51_12030"
     CDS             2080..3345
                     /locus_tag="PKN51_12030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNT85870.1"
                     /translation="MHPKALLDAASELVLRVLRLDHPADAVVSRFFREQRQLGPRERA
                     TLAETAYAVLRRKPLFEHLARSGSGGRERRLAILGFAAPRDFLKQALTDAEKHWLDAC
                     DAIAPAELPEACRHNLPAWLVEPLRAQLGDGFDALAQALLQGAPLDLRVNTMLQKREL
                     AAQELQALGIDSVPTPYSPWGLRLAGKPALTRLPPFQQGRIEVQDEGSQLLALLLDAR
                     RGEMVTDFCAGAGGKTLAIGAAMRSTGRLYAFDVSAHRLEALKPRLARSGLSNVHPVA
                     IAHERDERIKRLAGKMDRVLVDAPCSGLGTLRRNPDLKWRQTPEAVAELVSKQRAILA
                     SAARLLKPGGRLVYATCSLLTEENEAIAAAFGGEHTAFQPLPVAELLTGLKVTGAAAL
                     CTADGAYLRLWPHRHATDGFFAAAWQRRA"
     gene            3430..4323
                     /locus_tag="PKN51_12035"
     CDS             3430..4323
                     /locus_tag="PKN51_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNT85871.1"
                     /translation="MARDIRTVVSGMHFLEGPRWHDGRIWFSDFYSFQVYSAREDGSD
                     LRVEARVEHKPSGIGWLPDGRLLVVSMRDRKLLRREADGTLVTHADLSDYAGGNANDM
                     VVDAQGRAWVGDFGFDLMGGAPFRPTGLWRVDPDGTVHRAAEDMWFPNGAVITDDGTL
                     RVCETFANRVTAFDIGADGTLSRRRVWARFGEPPTSADVGEAIGQVAVAGDGCCLDAE
                     GAMWIADAVHGRVVRVREGGQIVDEIQPGTGAFACMLGGADGRTLFICAAPDFYEHAR
                     AAATEASLLSVRVDVPHAGRP"
     gene            4347..5498
                     /locus_tag="PKN51_12040"
     CDS             4347..5498
                     /locus_tag="PKN51_12040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT85872.1"
                     /translation="MVEADDAVLIIGAGIAGASLAWRLARAGRPVTLIEREPQPGMHS
                     TGRSAAMFMASYGPPGVRALTRASLDFYLQPPAGFAEQPLLSPRRALFVATAEQQPAL
                     ARMRADLTASGTTTTLLNSEQLALSAPALRPGMFQNALLDAQGYDIDVHALLQGFLRG
                     ARQAGARLRTGVEPQLARHDGQRWQVTLSDGSAWTGRTVVDAAGAWADQVAVLFGAAP
                     IGLEPRRRSAFTFRAPAGADLGQWPMVADVDEAWYFKPDAGQLLGSPANADPTIPHDV
                     QPEDYDIALGIHLIQEATTLRIDRPTATWAGLRSFVADGDLVIGFDQACPGFFWLAAQ
                     GGYGIQSAAGASLLAASQIVGEPLPAELAAHGVDEAVLSPRRLRRTAPS"
     gene            complement(5545..6177)
                     /locus_tag="PKN51_12045"
     CDS             complement(5545..6177)
                     /locus_tag="PKN51_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT85873.1"
                     /translation="MLSDPQAFAWPADASTVAAGTQLLRRACVDEHVLYIDSGRVALG
                     VVENGVLAHQMGLVEGPAWLNVGCAVLDKAPVVDAVADSPVQLRRVPLRDFVAGLREM
                     PAPAQALLRDMAAAHRQQAELAVSRLAKDAEARCAEWLLRHAEPAQGAPGTLAVLLRQ
                     RKRMIAAQLGIAPETLSRVLRQLRERSLISGSGRILNLLDPGALRALAGA"
     gene            complement(6292..6367)
                     /locus_tag="PKN51_12050"
     tRNA            complement(6292..6367)
                     /locus_tag="PKN51_12050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6332..6334),aa:Thr,seq:cgt)
     gene            complement(6481..>6577)
                     /locus_tag="PKN51_12055"
     CDS             complement(6481..>6577)
                     /locus_tag="PKN51_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HNT85874.1"
                     /translation="NYISATLALYLDLFNVFQGLLALLGLGGSND"
BASE COUNT          938 a         2362 c         2267 g         1010 t
ORIGIN      
        1 gatgccggtc tgcggcttgc cctcgttgct ggtgcgcacc aggttgaaca tccactcgcc
       61 atgctggccc agcgtggtcc aggtcttctg gccgttgacg atgtatttgt cgccgcggcg
      121 ctcggccttg gtgcgcaccg aggccaggtc ggaccccgaa cccggctcgc tgtagccctg
      181 gctccaccac acttcgcccg aggcgatgcc gggcagaaag cgcttttgct gctcgggcga
      241 gccgtaggcc atgatgaccg gggccaccat caccgggccg aagggcacga tgcgcggggc
      301 gcccgccatg gccagttctt cttcgaacag gtgtttctgc accgccgtcc agcccgggcc
      361 gccgaactgc ttgggccagc cgtagcccag ccagcctttc ttgcccagga tgcgggccca
      421 gctctgcatg tcgtcgcgcg tcaggcgctg cgcctggtgc accttgtcgg ccagcgcctt
      481 gggcagggcg tccttgaccc aggcgcggat ctcgtcgcgg aatttgagtt cgtcgggggt
      541 gaatgccaga tccatcagat cgtctccttg tcagccagca gttgtagcac gatcggtgcc
      601 ggcgtcacgt ccggcaggtg acaggcccag ctcggcgcac aggcgggcca cggtggcctg
      661 cagtgcggcc agctcggcct cgagcctttc caggcgttcg gtcgtggggt caaatcggcc
      721 tgcggcgctt tcggggtgtg cgccggcagc ttctttttca atagcaggtg ggccacccag
      781 cagatgcgtc cagcgcggct cgcgctcgcc cggtgcgcgc gccagcagca gcgtcagggc
      841 gccgcccttg tcggccggac gctcggccag ttcgtgcaga aagccttcga ccgaggagat
      901 gtcggcaaag cggtaccagc gctcggcgtt cagccgcagc tcggcggcgg tctgcgggcc
      961 gcgcagcatc aaaaggccca gcagcaccgc cgactgctcg ggcacggtca gggcgcgctg
     1021 caggttgtgt tcccagcgtg ccacgcggct gccgtgcgac tcgatcgcca ggtgcagcgc
     1081 cttcagcgcc tccagcgcgg cttgcgcctc ggcttcgctc aggttcatca ccggctcgcg
     1141 gctggtcttc tggttgcagc cgctcaccac ggcgttgagc gacagggggt aggtatcggg
     1201 cacggtgcgt gccttttcca tcagcgtgcc cagcacgcgc agctcggtcg gggtcagggg
     1261 acgcaggttc atcggccaag gataatccgg cccgacatga aacacatcgt gatcctgatc
     1321 tccggcaccg gcagcaacat ggcggccatc gtgcgcgccg ccgaacgcga agactgggcc
     1381 gcccggctgg gcgcgcgcgt ggcggcggtc atcagcaacc ggcctgacgc ccggggtctg
     1441 cagaccgcgc aagaggcggg catcgccacc gccgtggtcg atcacaggca gcatgccgac
     1501 cgcgccgcct tcgacgccgc gctgagggcc gagatcgacc gccatcagcc ggcgctggtg
     1561 gtgctggccg gcttcatgcg catcctcacg cccggcttcg tggcgcacta cgcggggcgc
     1621 atcgtcaaca tccaccccag cctgctgccc gcctttccgg gcctgcacac acaccagcgc
     1681 gccatcgaca tgcgctgccg cgtggccggc gccacggtgc atcaggtgac ggccgagctg
     1741 gaccacggcg ccattctggc gcaggccgcg gtgccggtgc tgccgggcga taccgcggac
     1801 accctggccg cccgggtgct gacgcaggag cacctgatct atccgcgcgc aatcgccgaa
     1861 ttgctatcta aaggatagct acctgcgcaa tttgatagag cgctggagcc aaatttggtt
     1921 catagtcggt atctctgaat cccagccaga ctccaggcga agactccgaa ggcatcccac
     1981 aagccgtttg ttttcttttg cgtcctctgc gaaccctctg cgcgctcggc gtaagggtgt
     2041 tctcgtccat tcaacccact cccgttccag ccgcccacca tgcaccccaa agccctgctc
     2101 gacgccgcca gcgaactggt gctgcgcgtg ctgcgcctgg atcacccggc ggacgccgtg
     2161 gtctcgcgct ttttccgcga gcagcgccag ctcggcccgc gcgagcgtgc caccctggcc
     2221 gaaaccgctt acgccgtgct gcgtcgcaag ccgctgttcg agcacctggc gcgctcgggc
     2281 tcgggtggac gcgagcggcg cctggcgatt ctgggttttg ctgcaccccg tgactttctc
     2341 aaacaggcct tgaccgacgc cgaaaagcac tggctcgatg cctgcgacgc catcgccccc
     2401 gccgagctgc ccgaggcctg ccgccacaac ctgccggcct ggctggtcga gccgctgcgc
     2461 gcacagctgg gtgatgggtt cgacgccctg gcgcaggccc tgctgcaggg cgcgccgctg
     2521 gacctgcgcg tcaacaccat gctacaaaaa cgggagctgg cggcgcagga actgcaagcg
     2581 ctgggcatcg attcggtgcc caccccttac tcgccctggg gtctgcgtct ggccggcaag
     2641 ccggcactga cgcggctgcc accctttcag cagggccgca tcgaggtgca ggacgaaggc
     2701 tcgcagctgc tggccctgct gctggacgcg cggcgcggcg aaatggtgac cgacttctgc
     2761 gccggtgccg gcggcaagac gctggccatt ggcgcggcca tgcgctccac cgggcggctg
     2821 tacgctttcg atgtgtcggc gcatcggctg gaggcgctca agccgcggct ggcgcgcagc
     2881 ggcctgtcca acgtgcaccc ggtggccatt gcgcatgagc gcgacgagcg catcaagcgc
     2941 ctggccggca agatggaccg cgtgctggtc gatgcgccgt gctcgggcct gggcacgctg
     3001 cgccgcaacc ccgacctcaa atggcgccag acgcccgagg cggtggccga gctggtgagc
     3061 aagcagcgcg ccatcctggc cagcgctgcg cgcctgctca agcccggcgg gcggctggtg
     3121 tacgccacct gcagcctgct gaccgaggag aacgaggcca tcgctgcagc gttcggtggc
     3181 gagcacaccg cgtttcagcc gctgccggtg gccgagctgc tgaccggcct gaaggtgacc
     3241 ggtgcggcgg cgctgtgcac ggccgatggt gcctacctgc gcctgtggcc gcaccggcat
     3301 gccaccgacg gcttctttgc cgccgcctgg cagcgccgcg cctgacgcct gcgggaccac
     3361 ccggcgcttg gacggcgcgg cacgcggcgt cacaatcggt cgcatccatc accgacaaag
     3421 gagcgtttca tggcacgcga cattcgcacc gtggtttcgg gcatgcactt tctcgaaggg
     3481 ccgcgctggc acgatggccg catctggttc tccgacttct attcgttcca ggtctattcg
     3541 gcgcgcgaag acggcagcga cctgcgcgtc gaggcgcgcg tcgagcacaa gccctcgggc
     3601 atcggctggc tgcccgacgg gcggttgctg gtggtgtcca tgcgcgaccg caagctgctg
     3661 cggcgcgagg ccgacggcac gctggtgacg cacgccgacc tgtcggacta cgccggtggc
     3721 aacgccaacg acatggtggt cgacgcccag ggccgcgcct gggtgggcga cttcggcttc
     3781 gacctgatgg gcggcgcgcc ttttcgcccc accggcctgt ggcgggtcga ccccgacggc
     3841 acggtacacc gcgccgccga agacatgtgg tttcccaacg gcgccgtgat caccgacgac
     3901 ggcacgctgc gcgtgtgcga aacctttgcc aaccgggtca cggccttcga catcggcgcc
     3961 gacggcacgc tcagccgccg ccgcgtctgg gccaggttcg gcgagccgcc caccagcgcc
     4021 gacgtgggcg aagcgatcgg gcaggtggcg gtggccggtg acggctgctg tctggacgcc
     4081 gaaggcgcca tgtggattgc cgacgccgtg cacggccgcg tggtgcgggt gcgcgagggt
     4141 ggccagatcg tcgacgagat ccagcccggc accggcgcct tcgcctgcat gctgggcggt
     4201 gccgacggcc gcacgctgtt catctgcgcc gcgcccgact tctacgagca cgcgcgcgcc
     4261 gccgccaccg aggcgagcct gctgtcggtg cgcgtggacg tgccgcacgc cggccggccc
     4321 tgagcgccga agcgagggca cgccccatgg tcgaagcgga cgacgcggtg ctcatcatcg
     4381 gcgccggcat cgccggcgct tcgctggcct ggcggctggc gcgcgccggc cggccggtga
     4441 cgctgatcga gcgcgagccg cagcccggca tgcacagcac cggccgttcg gccgccatgt
     4501 tcatggcaag ctacggcccg cccggggtgc gcgcgctgac ccgcgccagc ctcgacttct
     4561 acctgcagcc gccggccggt tttgccgagc agccgctgct gagcccgcgc cgcgccctgt
     4621 tcgtcgccac cgccgagcag cagcccgcgc tggcgcgcat gcgggccgat ctgacggcca
     4681 gcggcaccac caccacgcta ctgaattcag agcagctggc gctgtctgcg cctgccctga
     4741 ggcccggaat gtttcagaac gccctgctgg atgcgcaggg ttacgacatc gatgtccacg
     4801 ccctgttgca aggttttctg cgcggcgccc gccaggccgg cgcgcgcctg cggactggcg
     4861 tcgagccgca gcttgccagg cacgacggcc agcgctggca ggtgacgctg tcggacggca
     4921 gcgcctggac agggcgcacc gtggtcgatg cggccggcgc ctgggccgac caggtggcgg
     4981 tgctgttcgg cgccgcaccc atcggcctgg agccgcgccg gcgcagcgcc ttcaccttcc
     5041 gcgcgccagc cggcgccgac ctgggccagt ggccgatggt ggccgacgtc gatgaagcct
     5101 ggtatttcaa acccgacgcc ggccagttgc tgggctcacc cgccaacgcc gaccccacca
     5161 tcccgcatga cgtgcagccc gaagactatg acatcgccct gggcatccat ctgatccagg
     5221 aagcgaccac gctgcgcatc gaccgcccga ccgccacctg ggccggcctg cgcagcttcg
     5281 tggccgatgg cgatctggtg atcgggtttg accaggcttg cccaggcttc ttctggctgg
     5341 ccgcgcaggg cggctacggc atccagagtg cggccggcgc cagcctgctg gccgccagcc
     5401 agatcgtggg cgagccgctg ccggccgagc tggcggccca tggggtcgat gaggctgtgc
     5461 tgtcgccgcg ccggctgcgc cgcaccgcgc cgtcgtagcg cgcgccccgg ctgcggggcg
     5521 gggcagctgg cttgcggtgg tggctcaggc gcctgccagc gcccgcagag cgcccggatc
     5581 gagcaggttc aggatgcggc cggagccgct gatcaggctg cgctcgcgca actggcgcag
     5641 cacgcgcgac agggtttcgg gcgcgatgcc caactgcgcg gcgatcatgc gcttgcgctg
     5701 gcgcagcaac acggccagcg tgccgggcgc gccctgcgcc ggctcggcat ggcgcagcag
     5761 ccattcggcg cagcgcgcct cggcgtcctt ggccagccgg ctgaccgcca gttcggcctg
     5821 ctggcggtgc gccgccgcca tgtcgcgcag cagcgcctgg gccggcgccg gcatctcgcg
     5881 cagcccggcc acgaagtcgc gcagcggcac gcgccgcaac tgcaccgggc tgtcggccac
     5941 ggcgtccacc accggcgctt tgtccagcac ggcgcagccg acgttcagcc aggccgggcc
     6001 ttcgaccaga cccatctggt gcgccagcac gccgttttcg accaccccca gcgccacgcg
     6061 gccactgtcg atgtacaaca catgctcgtc gacgcaggcg cgccgcagca actgggtgcc
     6121 ggccgccacc gtgctcgcat cggcgggcca ggcaaaggct tgaggatcgg acaacatgcg
     6181 ggcactgtgc cgcgccgcaa ccggtggtgc tttgatgcac atcaacgggg cgcaccacca
     6241 cacccgccaa agacaaggcc cctggggaaa accaggggcc ttcggggggt ttggtgccgg
     6301 ctgccggaat cgaactggcg acctacgcgt tacgagtgcg ttgctctacc aactgagcta
     6361 agccggcgaa gacttgaaag ccgcgcatta tagccggatc agggtaccct gagacggtac
     6421 cggcgtggcg cccgtgcgcc accccatgca cgatccggcg gcaagcttgc aggccgtcga
     6481 tcagtcgttg ctgccaccca ggccgagcag ggccagcagg ccctggaaca cgttgaacag
     6541 gtccaggtac agcgccagcg tggcgctgat gtagttg
//
LOCUS       DAOQTZ010000128         6491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13098_length_6491_cov_13.722498, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000128 DAOQTZ010000000
VERSION     DAOQTZ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13098_length_6491_cov_13.722498"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..781
                     /gene="metW"
                     /locus_tag="PKN51_12060"
     CDS             197..781
                     /gene="metW"
                     /locus_tag="PKN51_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNT85875.1"
                     /translation="MSDEPIRRVIAGLVPEGAHVLDLGCGDGALLDHLLRQRRCTGYG
                     IEIDDANVLACVQRGVNVLQLNLHQGLAAFQDQSFDVVLQIDTLQHLRNAEVMLRETA
                     RVGRIGIIAFPNFAHWPNRLSVLRGRMPVTRRLPYQWYDTPNIRVGTFKDFEALARKN
                     GLQLLDAFGLEEGRVVRWLPNARASTAVFKLARR"
     gene            complement(789..2516)
                     /locus_tag="PKN51_12065"
     CDS             complement(789..2516)
                     /locus_tag="PKN51_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNT85876.1"
                     /translation="MAKVLIANRGEVAVRIARACRDYGVRSVAVYADPDADALHVRAA
                     DEAWALPGTRPADTYLAIDRLLAVARQSGADAVHPGYGFLSERAEFARAVLAAGLTWI
                     GPRPETIEALGDKVAARRIALQVGAPLVAGTAEPVGSADEVLAFARRHGLPLAIKAAY
                     GGGGRGLKVAWQMDEVVELYASAVREATAAFGRGECFLEQFLDRPRHIEAQVIADRHG
                     HVVVLGTRDCSLQRRHQKLVEEAPAPFLSDAQRAQVHQAARDICAAAGYESAGTVEFL
                     LGQSGQISFLEVNTRLQVEHPVTEETSGVDLVLEQLRVADGLPLSFTQAPPVRGHSFE
                     FRLNAEDPGRGYLPTPGRITRFDLPAGPGVRVDTGFAAGSTVPGSFDSLLAKLIVTGA
                     TRAQALARARRALAECRIEGVASVLPFHRAVVDDPAFVGADGLQVHTRWIETELQASF
                     EPAPRADGPDHAPLLRSFIEVDGRRLSLGLPAGLALGVGAAAAPAAPPSAAPTAEAGD
                     VLAPLTGSLVRWHRQTGEPVAAGEAIATLEAMKMETVVSAPCAGTLECLAAPGDMLTA
                     GQRLARVRG"
     gene            complement(2516..4174)
                     /locus_tag="PKN51_12070"
     CDS             complement(2516..4174)
                     /locus_tag="PKN51_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HNT85877.1"
                     /translation="MRLLPASDTALLVELADLEQTLALYRAAQAQPIDGVQELVPAAR
                     TLLVHYRPEAVTPTELAAALRARQAAAQAGGQPVAGTRAARQVEIVVRYDGEDLPAVA
                     ERLGLSVADVIARHTAQPWQAAFAGFAPGFVYLAGGHASLRGIPRRPSPRTRVPAGAV
                     ALAGDFSAVYPAASPGGWQLIGVTAVPMWDLRRAEPAYVQPGFQVQFIDQRTAGRHIS
                     LPAVPDSESNKPLAPDQQAPEAIEFEAVGLQTLVQDAGRHGMAGLGISPSGALDLPAM
                     RQANRLVGNPVHTPVLEHLLGGLRLRCHGRATLAVSGAVAPLTLHAPDGRQWPVPGHA
                     ALALDDGDVLAIGAPCAGVRCYVAVRGGWQVAPVLGSCATDTLAHVGPPALQAGQRLA
                     VGGAVPAQALRATQDAAAPAPELPRAGAQVTLDVVAGPRTDWFGPEALARLTQQPWQV
                     TPQSNRIGLRLHGAEPLARARHDELPSEGTVTGAIQVPAGGQPVLFLADHPLTGGYPV
                     IAAVVRHHLPLAAQLAAGCQLRFRLVAPFAELGDAHDTLPEKPA"
     gene            complement(4178..4936)
                     /locus_tag="PKN51_12075"
     CDS             complement(4178..4936)
                     /locus_tag="PKN51_12075"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNT85878.1"
                     /translation="MDINSDLGESYGAWRMGDDAAMLDIVTSANVACGFHAGDPAGIL
                     HTLRAAAARGVAVGAHVAYPDLVGFGRRAMDLGSDELVADVIYQIGALQGLAQAAGTR
                     VRYVKPHGALYNTIAHDARQGDDVISALLALDRKLVLLALAGSPLIGRARARGLTVVA
                     EAFADRAYNDDGTLVSRRLPGAVLHDADQVAERMLRWSESGTLTSISGRPVRLEAESI
                     CVHGDSPGAVAMARAVRARLSAAGVPLGAFIAAD"
     gene            5062..6420
                     /locus_tag="PKN51_12080"
     CDS             5062..6420
                     /locus_tag="PKN51_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNT85879.1"
                     /translation="MTDLPTIDQLSAELAAGRRSAVQLTEAALARAQDPAGEGARVFT
                     RLDPARALAAARASDQLRAAGIVRSPLEGIPVSIKDLFDVAGQVTTAGSAVLRDAPPA
                     ARSAPVVERLIAAGAVIVGRTNMTEFAFSGLGLNPHYGTPANPWQRAERRIPGGSSSG
                     AAVSVSDGMAAVAIGSDTGGSVRIPSALCGLTGFKPTQRRVPTAGALPLSTSLDSIGP
                     LGASVRCCALTDAVLAGQSLLPLPARPLRDARLALPDTLVFDDLEPAVAAAFERACAR
                     LQAGGARLQRIAVPEFAELGRLHARGTLAAAEAWAWHRQLLATRAAEYDPRVGLRLQG
                     GAQMSAADYLDLQAARRRWIEQVEARLAPFDALLLPTVPLLAPRIDALQASDADYFAA
                     NARMLRNPTLINFLDGCALSLPCHVPGEAPVGLMLAAAGGQDRALLALGETVEQALRP
                     AP"
BASE COUNT          808 a         2436 c         2434 g          813 t
ORIGIN      
        1 gataccgaat gagcgcgccg ggccgctccc aagcgcgaat cccgcagcgc gtcagcgcgg
       61 agggataccg aatgagcgcg ccgggccgct cccaagcgcg aatcccgcag cgcgtcagcg
      121 cggagggcac tccggtgagc gcgccgggcc gctcccaagc gcgaatcccg cagcgtgcag
      181 cgcggaggga tgttccatga gcgacgaacc gatccgccgc gtcatcgccg gtctggtgcc
      241 cgagggtgcg cacgtgctcg acctgggctg cggcgatggc gcgctgctcg accatctgct
      301 gcgccagcgc cgctgcaccg gctacggcat cgagatcgac gacgccaacg tgctggcctg
      361 cgtgcagcgc ggcgtcaacg tgctgcagct caacctgcac cagggcctgg ccgccttcca
      421 ggaccagagc ttcgacgtgg tgctgcagat cgacaccctg cagcacctgc gcaacgccga
      481 ggtcatgctg cgcgaaaccg cgcgcgtggg gcgcatcggc atcatcgcct tccccaactt
      541 cgcccactgg cccaaccgcc tgagcgtgct gcgcgggcgc atgccggtga cgcgccggct
      601 gccctaccag tggtacgaca cgcccaacat ccgcgtcggc acgttcaagg acttcgaggc
      661 gctggcgcgc aagaacggcc tgcagctgct ggacgccttt ggcctggagg agggacgggt
      721 ggtgcgctgg ctgcccaacg cccgcgccag cacggcggtg ttcaagctgg cgcggcgctg
      781 acggccgctc agccgcgcac gcgcgccagg cgctgaccgg cggtcagcat gtcgccgggc
      841 gcggccaggc attccagggt gccggcgcag ggcgcgctga ccacggtttc catcttcatg
      901 gcttccagcg tggcgatggc ttcgccggcc gccaccggct cgcccgtctg gcggtgccag
      961 cgcaccaggc tgccggtgag cggcgccagc acatcgcccg cctcggccgt cggggcggcg
     1021 ctcggggggg cggcgggggc ggcggcagcg ccgacgccca gcgccagacc ggcgggcagg
     1081 cccaggctca ggcgccggcc gtccacttcg atgaagctgc gcagcagcgg ggcgtgatcg
     1141 gggccgtcgg cgcgcggcgc cggctcgaag ctggcctgca gctcggtttc gatccagcgc
     1201 gtgtgcacct gcaggccgtc ggcgccgacg aacgccgggt cgtcgaccac cgcgcgatgg
     1261 aacggcagca ccgaggccac gccctcgatc cggcattcgg ccagcgcccg gcgcgcgcgc
     1321 gccagcgcct gcgcgcgcgt ggcgccggtg acgatcagct tggccagcag cgagtcgaag
     1381 ctgcccggca ccgtgctgcc ggcggcaaag ccggtatcga cccgcacgcc ggggccggcc
     1441 ggcaggtcga agcgcgtgat gcgcccgggc gtgggcaggt agccgcggcc cgggtcctcg
     1501 gcgttgaggc ggaattcgaa gctgtggccg cgcaccggcg gcgcctgggt gaacgacagc
     1561 ggcaggccgt cggccacgcg cagctgctcg agcaccagat cgacgccgct ggtttcttcg
     1621 gtcaccgggt gctcgacctg caggcgggtg ttgacttcga ggaacgagat ctggccgctc
     1681 tgcccgagca ggaattccac cgtgccggcg ctttcgtagc cggcggcggc gcagatgtcg
     1741 cgcgccgcct ggtgcacctg ggcgcgctgg gcgtcgctca gaaacggcgc cggcgcttct
     1801 tccaccagct tctggtggcg ccgctgcaac gagcagtcgc gggtgccgag caccaccacg
     1861 tggccgtgcc ggtcggcgat gacctgggcc tcgatgtggc gcgggcgatc cagaaactgc
     1921 tccagaaagc actcgccgcg cccgaacgcc gccgtcgctt cgcgcaccgc ggatgcgtac
     1981 agctcgacta cctcgtccat ctgccaggcc accttcaggc cccggccgcc gccgccgtag
     2041 gccgccttga tcgccagcgg caggccgtgt cggcgggcaa aggccagcac ctcgtcggcg
     2101 ctgcccaccg gctcggcggt gccggccacc agcggcgcgc ccacctgcag cgcgatgcgg
     2161 cgcgccgcca ccttgtcgcc cagcgcctcg atggtttccg ggcgtggccc gatccaggtc
     2221 aggccggccg ccagcaccgc gcgcgcaaac tcggcccgct cggacagaaa accgtagccc
     2281 gggtgcacgg cgtcggcgcc ggactggcgg gccacggcca gcagccggtc gatggccagg
     2341 taggtgtcgg ccgggcgtgt gcccggcagc gcccaggctt catcggcggc gcgcacgtgc
     2401 agggcgtcgg cgtcggggtc ggcgtacacc gccaccgagc gcacgccgta gtcgcggcag
     2461 gcgcgcgcga tgcgcacggc aacttcgccg cggttggcga tcaggacctt ggcaatcatg
     2521 cgggcttctc cggcagggtg tcgtgggcat cgcccagttc ggcaaacggg gcgacgaggc
     2581 gaaagcgcag ctggcagccg gcggcaagct gggccgccag cggcaggtgg tggcggacga
     2641 cggcggcgat caccgggtag ccgccggtca gcgggtggtc ggccaggaac agcaccggct
     2701 ggccaccggc cggcacctgg atggcgccgg tgaccgtgcc ttcgctgggc agctcgtcgt
     2761 ggcgcgcgcg cgccagcggc tcggcgccgt gcaggcgcag gccgatgcgg ttggactgcg
     2821 gcgtgacctg ccacggctgc tgcgtcagcc gcgccagcgc ctcggggccg aaccagtcgg
     2881 tgcgcgggcc ggccaccacg tccagcgtga cctgcgcgcc cgcgcgcggc agctcgggcg
     2941 ccggcgctgc ggcgtcctgg gtggcgcgca gcgcctgcgc cggcaccgcc ccaccgacgg
     3001 ccaggcgctg gccggcctgc agcgccggcg ggccgacatg ggccagggtg tcggtggcgc
     3061 agctgcccag caccggcgcc acctgccagc caccgcgcac cgccacgtag cagcgcacgc
     3121 cggcgcaggg tgcgccgatg gccagcacgt cgccgtcgtc cagcgccagc gcggcatggc
     3181 cgggcaccgg ccactggcgg ccgtccggcg cgtgcagggt cagcggcgcc acggcgccgc
     3241 tgaccgccag cgtggcgcgg ccgtggcagc gcaggcgcag gccgcccagc agatgctcca
     3301 gcaccggcgt gtgcaccggg ttgccgacca gccggttggc ctggcgcatg gcgggcagat
     3361 ccagcgcgcc cgagggcgat atgcccagcc cggccatgcc gtggcgaccc gcgtcctgca
     3421 ccagagtctg taggccgacc gcttcaaatt cgatagcttc tggcgcttgc tggtcgggcg
     3481 ccagaggctt gtttgattcg gaatccggca cggccggcag gctgatgtgg cggccggccg
     3541 tgcgctggtc gatgaactgc acctgaaagc ctggctgcac ataggcgggt tcggcgcgcc
     3601 gcaggtccca catcggcacc gcggtgacgc cgatcagctg ccagccgccg ggcgaggcgg
     3661 ccgggtagac ggcgctgaag tcgcccgcca gcgccaccgc cccggccggc acgcgggtgc
     3721 gcgggctggg ccggcgcggg atgccgcgca ggctggcgtg gccgccggcc aggtaaacga
     3781 agccgggcgc gaacccggcg aacgccgcct gccagggctg ggccgtgtgg cgggcgatca
     3841 cgtccgccac cgacaggccc agccgttcgg ccacggcggg caggtcttcg ccgtcgtagc
     3901 gcaccacgat ctcgacctgg cgggcggcgc gggtgccggc caccggctgc ccgccggcct
     3961 gggccgcggc ctgccgcgcg cgcagcgccg ccgccagctc ggtcggcgtc accgcctcgg
     4021 gccggtaatg caccagcagc gtgcgcgcgg ccggcaccag ctcctgcaca ccgtcgatcg
     4081 gctgggcctg ggcggcgcgg tacagcgcca gggtctgctc gaggtcggcc agttcgacca
     4141 gcagcgcggt gtcgctggcg ggcagcaagc gcatggctca gtccgcggcg atgaaggcgc
     4201 ccagcggcac gccggcggcg ctcagccgcg cgcggacggc gcgtgccatc gccaccgcgc
     4261 cggggctgtc gccgtgcacg cagatcgact cggcttcgag gcgcaccggc cggccgctga
     4321 tcgacgtcag cgtgccgctt tcgctccagc gcagcatgcg ctcggccacc tggtcggcgt
     4381 cgtgcagcac ggcgccgggc aggcggcgcg acaccagggt gccgtcgtcg ttgtaggcgc
     4441 ggtcggcaaa ggcctcggcc accacggtca ggccgcgcgc gcgggcgcgc ccgatcagcg
     4501 gcgagccggc cagcgccagc agcaccagct tccggtcgag cgccagcagg gcgctgatca
     4561 cgtcgtcacc ctggcgcgcg tcgtgggcga tggtgttgta gagcgcgccg tgcggcttga
     4621 cgtagcgcac ccgcgtgccg gcggcctgcg ccaggccctg cagggcgccg atctggtaga
     4681 tcacgtcggc caccagctcg tcgctgccga ggtccatggc gcggcggcca aagcccacca
     4741 ggtcggggta ggccacgtgc gcgcccaccg ccacgccgcg cgcggcggcg gcgcgcagcg
     4801 tgtgcaggat gcccgccggg tcgcccgcgt ggaagccgca ggccacgttg gcgctggtca
     4861 cgatgtccag catggcggcg tcgtcgccca tgcgccaggc gccgtagctc tcaccgaggt
     4921 cgctgttgat gtccatgacc gcatctcctg aaggggccgt ccgccgtgcg cgcgggccga
     4981 ccgcatgctc gccattctgg cgcagcggcg gcgcgggctt accatgccgc tcggttgccc
     5041 tcttcatcca gcgtttcgac catgaccgat ctgcccacca tcgaccagct gtcggccgaa
     5101 ctggccgccg gccgccgcag cgccgtgcag ctcaccgagg ccgcgctggc gcgcgcacag
     5161 gacccggcgg gcgaaggcgc gcgggtgttc acccggctcg acccggcgcg cgcgctggcc
     5221 gccgcgcgcg cgtccgatca gctgcgcgcg gccggcatcg tgcgctcgcc gctggagggc
     5281 attccggtgt cgatcaagga cctgttcgac gttgccgggc aggtcaccac cgccggctcg
     5341 gcggtgctgc gcgacgcgcc gccggcggcg cgcagcgccc cggtggtcga gcgcctgatc
     5401 gccgccggcg cggtgatcgt gggccgcacc aacatgaccg aattcgcctt ttccggcctg
     5461 ggcctgaacc cgcactacgg cacgccggcc aacccgtggc agcgggccga gcggcgcatt
     5521 ccgggcggct ccagcagcgg cgcggcggtg tcggtgagcg acggcatggc ggcggtggcc
     5581 atcggctccg acaccggcgg ctcggtgcgc atcccgtcgg ccctgtgcgg cctgaccggc
     5641 ttcaagccga cccagcggcg cgtgcccacg gcgggcgcgc tgccgctgtc gacgtcgctc
     5701 gactccatcg ggccgctggg cgccagcgtg cgctgctgcg ccctcaccga cgccgtgctg
     5761 gccggccagt cgctgctgcc gctgccggcc cgcccactgc gcgacgcgcg cctggcgctg
     5821 cccgacaccc tggtgttcga cgacctggag ccggcggtgg cggccgcctt cgagcgcgcc
     5881 tgcgcccgtc tgcaggctgg cggcgcccgc ctgcagcgca tcgccgtgcc cgaattcgcc
     5941 gagctcggcc gcctgcatgc gcgcggcacc ctggcggcgg ccgaggcctg ggcctggcac
     6001 cgccagctgc tggccacccg cgcggccgaa tacgacccgc gcgtgggcct gcgcctgcag
     6061 ggcggggcgc agatgagcgc ggccgactac ctggatctgc aggcggcgcg gcgccgctgg
     6121 atcgagcagg tcgaagcgcg cctggccccg ttcgacgccc tgctgctgcc caccgtgccg
     6181 ctgctggcgc cgcgcatcga cgcgctgcag gccagcgacg ccgactactt cgccgccaac
     6241 gcgcggatgc tgcgcaaccc gacgctgatc aactttctgg acggctgcgc gctgtcgctg
     6301 ccctgccacg tgcccggcga ggcgccggtg ggcctgatgc tggccgccgc cggcgggcag
     6361 gaccgcgcgc tgctggcgct gggcgagacg gtcgagcagg ccctgcgccc ggcgccctga
     6421 gccgaaccat cgaatgcggc atcgaatcgg cgtccagcgc tttggtggca agcgtgagca
     6481 gctatcgttt g
//
LOCUS       DAOQTZ010000129         6480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13132_length_6480_cov_11.509105, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000129 DAOQTZ010000000
VERSION     DAOQTZ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13132_length_6480_cov_11.509105"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..636)
                     /locus_tag="PKN51_12085"
     CDS             complement(124..636)
                     /locus_tag="PKN51_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HNT85880.1"
                     /translation="MSAGKPTPLVIHGWTVFAHPLFLAQIEALTQQVEALKQKDQAGY
                     VKKNASKRLAAITKLAFDVIPQDPARPEYRQGSTLGDEHKHWFRAKFFEQYRLFFRYH
                     APSKVIVFAWVNDEDSKRAYESGDDAYRVFRKMLATGHPPDDWSQLLEEAKGAHDRLQ
                     SSMASQRALT"
     gene            complement(633..953)
                     /locus_tag="PKN51_12090"
     CDS             complement(633..953)
                     /locus_tag="PKN51_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HNT85881.1"
                     /translation="MAATLEVESTLTDRYQTTVPETVRRALRLGKRDKIHYSIRPSGE
                     VVLTRAEGDDPVLGQFLGFLARDIASHPERLQAVDAGLVQRLHSLVGGIEVDLDATLS
                     ADDE"
     gene            complement(1196..2428)
                     /locus_tag="PKN51_12095"
     CDS             complement(1196..2428)
                     /locus_tag="PKN51_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNT85882.1"
                     /translation="MPVPTFLAWCATLLLPVVVAHLPLAHAQPAPPHTDAAEFRACLQ
                     GLREAPAFRAVTPATFATHTDGLVPDASVLLLLDRQPEFTLAPWDYLAVLVDAERVAD
                     GRAGYARWQDTLAQIERQTGVAAPIVVGVWGVESNFGQNLGGRPLVQSLATLSCFGRR
                     QGYFRGELAAALRILQEGHVAPQQFNGSWAGAFGQTQFMPSTFLRSAVDFDGDGRRDI
                     VGSVPDALASTARFLQTAGWRRGEPWGFEVRLPAGFDGGGGRKHKRPLADWRRLGLTL
                     PSGAPLPDTLATAGLLQPARGGPAFLVGRNFDALYSYNASENYALAIAHLADLVAHAD
                     APLAFATPWPTDDPGLSRAENRELQTLLLARGHDIGAADGLIGDKTRAAIRQEQQRLG
                     LKTDGRAGQKLLDALRRP"
     gene            2739..3755
                     /locus_tag="PKN51_12100"
     CDS             2739..3755
                     /locus_tag="PKN51_12100"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT85883.1"
                     /translation="MDCFITGTAGFIGFHLAKLLLEQGHTVHGYDGITDYYDVRLKHA
                     RHNLLRQYPQFTATEAMLEDDATLQAAVAAARPSAIVHLAAQAGVRYSIEHPRSYIDA
                     NIIGSFNVMEAARRQQVAHLLMASTSSVYGANTEMPFDENQKCDMPMSFYAATKKANE
                     AMAHSWAHIHGVPITLFRFFTVYGPWGRPDMALFKFTKAILDGQPIDVYNHGDMWRDF
                     TYVEDLVRAIALLIDAVPPAAAGRNPAQAIEGDSLSPVAPWRVVNIGNSDKVRLLDFI
                     DAIEQCLGQKAIRNHLPIQPGDVPASWADTQLLQRLTGYKPATGFQQGVQAFVRWYCE
                     HHRP"
     gene            complement(3819..5744)
                     /locus_tag="PKN51_12105"
     CDS             complement(3819..5744)
                     /locus_tag="PKN51_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNT85884.1"
                     /translation="MSLQGLTHRLIRMPRAGKRLLALTLDAFLCAFTVWLAWALRLEH
                     WVPFGRMHAWATLGSWLLGLPVFAHFGLYASIFRYAGWNATLALGKAVAVYGGLYALV
                     FTLIAVPGIPRSIGIIQPLLMFVALGLSRVTVRLWIGDLYSELLARLNAPGVLIYGAG
                     QSGLQLAASLLRMHQQRLVGYLDDNPALQGRRLDGLPVFAPSDLDRLVRVYQVGSVLL
                     AIPSASLARRKQIVELLRPHPVHVRTLPGMADLVRRGVHASDLQDLDIEDLLGRDPVR
                     PDEALVEQQLSGRTVMVTGAGGSIGSELCRQILVWRPRVLLLLEQSEYALYEIHRELL
                     AAMGEPDDADGPLHIVPLLGSVQDAARLDAILTEWHPDTVYHAAAYKHVPLVEHNPIE
                     GLRNNVWGTLECAASAERHGVGHFVLVSTDKAVRPTSVMGASKRLAEMVLQALHAQGE
                     RAGGSGRTCFTMVRFGNVLGSSGSVVPLFRQQIEQGGPVTVTHREITRYFMTIPEAAQ
                     LVIQAGAMACGGEVFVLDMGEPVRIHDLACRMIELSGQRVRRPQDAPDAEGIEIRVTG
                     LRPGEKLYEELLSDDTSVRTAHPRILKAQERFLPWDQLQTWLEQLAEHMRARRHDDIR
                     ALLQQLVEGYRPSDRTD"
     gene            complement(6129..>6480)
                     /locus_tag="PKN51_12110"
     CDS             complement(6129..>6480)
                     /locus_tag="PKN51_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT85885.1"
                     /translation="LARLVQIDYTREMAFIATLPAPEGGEQTLGVARALADPDNLGAE
                     FGIILRPELKGSGLGRLLMDKLIRYQRGAGTQRLIASALVENWRMRELARALGFAEAP
                     TPGEPDTLRLTLEL"
BASE COUNT         1060 a         2250 c         2146 g         1024 t
ORIGIN      
        1 ccaccagact tccgcgccca acctctatcg gttgggcgtt ttcatttccg ggttccgcga
       61 cgccacgcgg ggttcagcgc cgatccgcct gtgccacgcg ttggcggccc ggcctgctgt
      121 tcgtcatgtc agagcccgct gtgaggccat gctggattgc aaccgatcat gggcgccttt
      181 ggcttcttcc agaagttggc tccaatcgtc gggcgggtgc cccgtggcca gcatctttct
      241 gaacacccgg taggcatcgt caccgctctc gtaggcacgc ttgctgtcct cgtcgttcac
      301 ccatgcgaac acaatcacct tgctgggcgc gtggtaacgg aagaacagcc ggtactgttc
      361 gaagaacttc gctcggaacc agtgtttgtg ttcgtcgccc aaggtgctgc cttggcgata
      421 ctccggtcgg gccggatctt gcgggatgac atcgaaggcc aacttcgtga tggctgccag
      481 tcgcttgctg gcattcttct tcacgtaccc ggcctggtct ttctgcttga gagcctcgac
      541 ctgctgagtc aacgcctcaa tttgtgcgag gaacaacggg tgggcgaaaa ccgtccagcc
      601 gtgaatgacc agcggcgtgg gcttgcccgc gctcattcat catctgccga cagggtggca
      661 tcgagatcga cttcgatgcc accgaccaag gaatggagac gttggacaag acccgcatcc
      721 acggcctgca ggcgctcggg atggctggcg atgtcccgcg ccagaaaacc gaggaactgg
      781 ccgagcaccg ggtcgtcgcc ttcggcgcgg gtcagcacca cttcaccgct ggggcgaatg
      841 ctgtagtgga tcttgtcgcg cttgcccaac cggagggcgc ggcgcacggt ttctggcacc
      901 gtggtctggt agcggtcagt cagcgtggat tcgacttcga gggtggcagc catggcgctc
      961 ttcaatcaaa agggtgaacg ccttgcatgg taatgcaatc gccttaccat gtcaatgcaa
     1021 atgcattgcc tgagcctgga aacggcagtt gcccgcttgt caacttcggg tggtccgcat
     1081 cgatgcgggg actcgcggct gcgatggcct tcacctcctg agtgagcacg ctgcgcaccc
     1141 acccgcaccg accgggcctg cggcgcactc gatcgacgcc atcggactgc ggcgctcagg
     1201 gtcggcgcag cgcgtccagc agcttctggc cggcgcggcc gtcggtcttc aggcccaggc
     1261 gctgctgctc ctggcggatg gcggcgcgcg tcttgtcgcc gatcaggccg tcggccgcgc
     1321 cgatgtcgtg cccgcgggcc agcagcaggg tttgcagctc gcggttttcg gcgcgcgaca
     1381 ggcctgggtc gtcggtcggc cagggggtgg caaaggccag gggcgcgtcg gcatgggcca
     1441 ccagatcggc caggtgcgca attgccagcg cgtagttctc gctggcgttg tagctgtaca
     1501 gcgcgtcaaa gttgcgcccc accagaaacg ccggaccgcc ccgtgccggc tgcagcagac
     1561 cggcggtcgc cagggtgtcg ggcagcggcg cgccgctggg cagcgtcaga cccaggcggc
     1621 gccagtcggc cagcggccgc ttgtgtttgc ggccgccgcc gccgtcaaac ccggccggca
     1681 gccgcacctc aaagccccag ggctcgccgc gccgccagcc ggcggtttgc aagaacctgg
     1741 ccgtggacgc cagcgcgtcc ggcacgctgc ccacgatgtc gcgccggcca tcgccgtcga
     1801 agtccaccgc gctgcgcaag aaggtgctgg gcatgaactg cgtctgccca aaggcgccgg
     1861 cccaggagcc attgaactgc tgcggtgcca catgcccctc ctgcagaatg cgcagcgccg
     1921 ccgccagctc gcccctgaag tacccctgcc gccgcccaaa gcacgacagc gtggcgagcg
     1981 actgcaccag cggccgcccg cccaggttct ggccgaagtt gctctccacc ccccacacgc
     2041 cgaccacgat cggcgccgcc acgccggtct gccgttcgat ctgcgccagc gtgtcctgcc
     2101 accgggcgta gccggcgcgg ccgtcggcca cccgttcggc gtccaccagc accgccaggt
     2161 aatcccaggg cgccagggtg aactcgggct ggcggtccag cagcagcaac accgacgcat
     2221 ccggcaccag gccgtcggtg tgggtggcaa aggtggcggg cgtgacggcg cgaaacgccg
     2281 gcgcctcgcg cagcccctgc aggcaggcgc gaaactccgc cgcgtcggtg tgcggcggcg
     2341 ccggctgggc gtgggccagt ggcaggtggg ccaccaccac gggcagcagc aaggtggcgc
     2401 accaagccag gaaggtcggg acaggcatga cagggtccgg atgctgtgtt ctccgtgatt
     2461 gtgaccgcgc gctggccaca ctcggccagc cggaatagct gccgcgccgc accaggaagc
     2521 ggtggggcgc acggcgcaat catgtgtatt cagactgctg ggtggccgtc gcgatccggt
     2581 cgaccatgct gctcatgtgc agcacgccca tcggccgccg gcgtcacggg gggcacccga
     2641 ctcaggtcat ggattcaagt aaaatcacgc cccagggctt actcaacaag cgccgtaagc
     2701 tatcattacg gtagcatcca acacctctgg tatctttcgt ggactgcttc atcaccggca
     2761 ccgccggctt catcggcttt cacctggcca aactgctgct ggagcagggc cacaccgtgc
     2821 acggctacga cggtatcacc gactattacg acgtgcgcct gaagcacgcc cggcacaacc
     2881 tgctgcgcca gtacccgcaa ttcacggcca ccgaggccat gctggaagac gacgcaaccc
     2941 tgcaggctgc cgtggccgcc gccaggccca gcgccatcgt ccacctggcc gcccaggccg
     3001 gcgtgcgcta cagcatcgag cacccgcgca gctacatcga cgccaacatc atcggcagct
     3061 tcaacgtcat ggaggccgcg cgccgccagc aggtggcgca cctgctgatg gcctccacct
     3121 cgtccgtcta cggcgccaac accgagatgc cgtttgatga aaaccagaag tgcgacatgc
     3181 caatgtcgtt ctacgccgcc accaagaagg ccaacgaggc catggcgcac agctgggccc
     3241 acatccacgg cgtgcccatc accctgtttc gcttcttcac cgtgtacggg ccctggggcc
     3301 ggcccgacat ggccctcttc aaattcacca aggccatcct ggacggccag cccatcgacg
     3361 tctacaacca cggcgacatg tggcgcgact tcacctatgt ggaagacctg gtgcgcgcca
     3421 tcgccctgct gatcgacgcc gtgccgcccg cggccgcagg ccgcaacccg gcacaggcca
     3481 tcgagggcga cagcctctcc ccggttgcgc cctggcgcgt ggtcaacatc ggcaacagcg
     3541 acaaggtgcg cctgctggac ttcatcgacg ccatcgaaca gtgcctgggc caaaaggcca
     3601 ttcgcaacca cctgcccatc caacccggcg acgtgcccgc ctcctgggcc gatacccaat
     3661 tgttgcagcg gttgaccggt tacaagccag ccaccggctt tcagcagggg gtgcaggcgt
     3721 ttgtgcgttg gtactgcgag caccatcgcc cgtagaccga ctctggcgcg cgccctggcc
     3781 tgtgtgtggt gcaggtgggc cgcgccgccg tgtcgaggtc agtctgtccg atcgctgggc
     3841 cgatagcctt ccaccaactg ctgcagcagg gcacggatgt cgtcgtgtcg ccgtgcccgc
     3901 atgtgctcgg ccagctgctc gagccaggtc tgcaactggt cccagggcag aaagcgctcc
     3961 tgcgccttca ggatgcgggg gtgggcggtg cggacgctgg tgtcgtcgct cagcagctct
     4021 tcgtacagct tttcgccggg acgcaggccg gtgacgcgaa tctcaattcc ttctgcgtcg
     4081 ggcgcgtcct gcggccggcg tacccgctga ccggacagct cgatcatgcg gcaggccagg
     4141 tcgtggatgc gcaccggctc gcccatgtcc agcacgaaca cctcgccccc gcaggccatg
     4201 gcgccggcct ggatgaccag ctgtgccgct tccggaatgg tcatgaaata gcgcgtgatc
     4261 tcgcgatgcg tgacggtcac cggtccgccc tgctcgatct gttgccgaaa cagtggcacc
     4321 accgagcctg aggagcccag cacattgccg aagcgcacca tggtgaagca ggtgcggccg
     4381 ctgccaccgg cccgctcgcc ctgcgcatgc agcgcctgca gcaccatttc ggccagccgt
     4441 ttgctggcgc ccatgacgct ggtggggcgc accgccttgt cggtgctgac cagcacgaaa
     4501 tgcccgacac catggcgctc agccgatgcg gcgcactcca acgtgcccca gacgttgttg
     4561 cgcagcccct cgatcgggtt gtgctcgacc agcggtacat gtttgtaggc ggcggcgtgg
     4621 tagacggtgt cggggtgcca ctcggtcagg atggcgtcca ggcgcgccgc atcctgcacc
     4681 gaacccagca gcggtacgat gtgcagcggg ccgtcggcgt cgtcaggctc gcccatcgcc
     4741 gccagcagtt cgcggtggat ctcgtacaac gcgtattcgc tctgctccag cagcagcagc
     4801 acgcgcgggc gccagaccag gatctggcgg cacagctcgc tgccgatgga gccaccggca
     4861 cccgtgacca tcaccgtgcg gccgctcagc tgctgctcca ccagggcttc gtccgggcga
     4921 accgggtcgc gacccagcag gtcttcgatg tccaggtcct gcagatcgct ggcgtgcacg
     4981 ccgcgccgca ccaggtcggc catgccgggc aaggtgcgca cgtgcaccgg atgggggcgc
     5041 agcagctcga cgatctgctt gcgccgtgcc agactggccg aggggatggc cagcagcacc
     5101 gacccgacct ggtacacgcg caccagccga tccagatcgg acggggcgaa caccggcagg
     5161 ccatcgagcc ggcggccctg cagcgcgggg ttgtcgtcca gatagccaac caggcgctgc
     5221 tggtgcatgc gcaacaggct ggcggccagt tgcaggcccg actggccggc gccgtagatc
     5281 agcacgccag gggcgttgag tcgggccagc agctcgctgt acaggtcgcc gatccacagc
     5341 cgcacggtga cccggctcag gcccagggcg acgaacatca gcagcggctg gatgatgcct
     5401 atcgagcgcg ggataccggg caccgcgatc agcgtgaaca ccagcgcgta cagccccccg
     5461 tacaccgcga ccgccttgcc cagcgccagc gtggcgttcc agccggcgta gcgaaagatc
     5521 gaggcataca gcccgaagtg agcaaacacc ggcaggccca gcagccacga cccgagcgtg
     5581 gcccaggcat gcatgcgacc gaacggcacc cagtgctcca gccgcagcgc ccaggccagc
     5641 cagacggtga aggcgcacag aaaggcgtcc agcgtcaggg ccagcagcct cttgccggca
     5701 cgcggcatgc ggatcagtcg gtgggtcagg ccctgcaagc tcatgccagc gttcgcgcgt
     5761 gcggaagtgc caggtggggg cccgctgacc ggcggggtgc aggagttcgg gcgagccgtc
     5821 cagccgtcat gcgggtttcg agggatgcgc aggcccaggg tgagtcgatc gaatgccagc
     5881 ccgatcggcc tggcgtgcgc gatcttaccc cccgcgtgcg cggcgtcgac ggcgcagggc
     5941 cggagcgcgc gcagtcgatg gctgccgctt catgcggggt gcggtcgaat ggctggcgcc
     6001 tgatccagcg ccgatgggca tggcgccgtc ccgtgaactt caaaatccat agctgctggc
     6061 gctcgccaga cgggcgccag aggccagaaa tccttgaaac ctacagatca ggcggcggca
     6121 ctgggcgcct acagctccag cgtcaggcgc agggtgtcgg gctcgcccgg ggtgggcgct
     6181 tcggcaaagc ccagcgcgcg tgccagctcg cgcatgcgcc agttctcgac cagggcgctg
     6241 gcgatcaggc gctgggtgcc ggcgccgcgc tggtagcgga tcagtttgtc catcagcagc
     6301 cggcccaggc cggagccctt gagttccggc cgcaggatga tgccgaattc ggcgccgagg
     6361 ttgtccgggt cggccagcgc gcgcgccacg cccagggtct gctcgccgcc ttcgggcgcg
     6421 ggcagggtgg cgatgaaggc catctcgcgc gtgtagtcga tctgcaccag ccgcgccagc
//
LOCUS       DAOQTZ010000130         6433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13265_length_6433_cov_7.851207, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000130 DAOQTZ010000000
VERSION     DAOQTZ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13265_length_6433_cov_7.851207"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..3459)
                     /locus_tag="PKN51_12115"
     CDS             complement(136..3459)
                     /locus_tag="PKN51_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNT85886.1"
                     /translation="MTPAYEHHGQPCSRERFYAIACDPRRHVAVEACAGAGKTWMLVS
                     RMLRALLAGSAPHEILAITFTKKAAGEMRQRLHEWLADFARARPDESPDAWDERLQAA
                     LIARGIEPQAAPDQRQQLQNLYHSVLGHGRPVQIRTFHSWFVALLKCAPLAVLQQLGL
                     PTAFELLEDDADAVAEVWRRYLRQLAGDAGLSADYHALVGALGRQRAHQALESALARR
                     VEFQLADAAGHVEAAVAPAGALYPEFAPFAHPCDALLTEAARERWLARARQLGPEKNK
                     TPQKAAAAVLDAFDAATDGRQRLAALRKAFFVASEDRLAKNLQSYGAAQQAEAELQRL
                     LPATAQHDAHQHHQRMVRLLRPLIDALARLKRERGWVDMPDVERAAFTLLADHELSAW
                     VQQRLDARIRHLLIDEFQDTNPLQWQALHAWLSGYAGAGGGPGAPSVFIVGDPKQSIY
                     RFRRADPQVFADAQRFIVTALGGELLACDHTHRNAPALLQAINGVMGQAQAAGEFSGF
                     RPHTTQATAPGALGALPLVPRPDKATAAGAPAPWRDSLVSPRLEPDETLRTLECRQAS
                     RWIAGRIATGVAPAQIMVLARKRDALGPLQAELRRLGIACDQPEKQLLGQQPAVQDVL
                     ALVDALLSPGHDLMLARALKSPLFGLGDDDLTRLALAAQAAQRAGQPRPGWLEVLSIT
                     ELSAHDGQALHADLMQYREGLLTLPPHDALQAMFEHRDVLARFAAAAPAPERAQVLIQ
                     LRALLAAALNLQGGRFLTAYQWLRALRRQRLPVPRAAAPGVVQLLTVHGAKGLEADEV
                     LLLDAAAGPPRGNGAAVLIDWRGAEPAPRCLAFLAQGGQPPPSLAALAQQEDQAQARE
                     ELNALYVAMTRARQRLVLSGITPHAQPASSWWQRIEPLAEPLGEPLAAPAADGAGVAG
                     TVPADAAFIMLDLPAAPAQQAPAAIKNEATEPSPEPPSSDSARVGEAMHWLLEQAPDG
                     AAGWHARRLLQARRRFGLDAAQAARAEALARRILTGEAAWAWDADEVLEAFNEVELVH
                     QGQRLRIDRLLRRRAGPHGPEAWWVLDYKSAAQPERDQALQAQLERYRAAVQQLHPGQ
                     PVQVAFLTGAGGVAVQGDG"
     gene            complement(3946..6432)
                     /locus_tag="PKN51_12120"
     CDS             complement(3946..6432)
                     /locus_tag="PKN51_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNT85887.1"
                     /translation="MLAEVRQQLHQLAAHPARCVVLLPFAQLLPLAAAWWAREHPDGF
                     APRFETTRSWAARVGMFEPGPNDLSFDRGRDLLSAAALLQGAGLGAQRAGLAAPLVEQ
                     AQQLAQVAAGLPPALRPDWAERARAAMPPAGDGPLGLEGALGRIALAWAAHSDYATDV
                     LFEPRVMRALDALVIVEGLQPDPLTATLAAQGVDKLVRLSLAGDVARGRVALHACADG
                     EDEAARAAACVLQHLQAGRTPVALVATDRVLQRRIHALLAARGVTAGGLLRDETGWRL
                     STSHAAARVMAALRACAPQASSDEVLDWLKLAPVIDPQALGRLEHRLRRDAVRGWAQA
                     VRLTSGEPLTQQVEALRAPLIGTRRVCDWLAALRALLQGCGLWAPLVQDVAGQAVVAA
                     LGLDDEALADWRDWPAAQRRVGLAEFSRWVADALEGASFRPSPSGSAQVVVLPLAQLP
                     GRPFAALVLPGADEQRLPAAPEPPGPWSAAQRAALGLPTREALMWAQAATWAQALGVP
                     AVDLLWRRGDDSGEPLQASPLVQVLQLGGHALDVGAAAEPRLPRPIAAAPGARPAPSG
                     AALPLQPLSASSYDMLRACPYRFFALHQLGLQDEGELDVELDKRDWGNWLHATLRAFH
                     EALQGAPQADRRALIDAAAEHTTEALGLDQATGEFMPFAAAWPALRDAYLGWLRDHEA
                     GGARFEAAEQPMQRVCGTLQLTGRIDRIDRLADGTRLLIDYKTEALKTSSQRVQAGSE
                     ETQLPFYALLSGADAPRAAYLNLAERESQPHLLELPELPELAARLYDGMAHDVARIAA
                     GAALPALGEGRVCDWCAARGLCRRDFWGAG"
BASE COUNT          841 a         2318 c         2452 g          822 t
ORIGIN      
        1 acaccggcgg ccgacagtcg ctcggccgcc atcaggccgg cggggccggc gccgacgatg
       61 gcgatgacga tggggtctgg ttcagccgat gccatggcac atgggtcgtt gaggaaagca
      121 cactttcggg gacggctagc catcgccctg tacagccacg ccacctgcgc cggtcagaaa
      181 cgccacctgc accggctgcc cggggtgcag ctgctgcacg gcggcgcggt agcgttccag
      241 ttgcgcctgc agggcctgat cgcgctcggg ctgcgcggcg ctcttgtagt ccagcaccca
      301 ccaggcctcg ggcccgtggg ggccggcgcg gcggcgcagc aggcggtcga tgcgcaggcg
      361 ctggccctgg tgcaccagct cgacttcatt gaaggcttcc agcacctcgt ccgcatccca
      421 ggcccaggcg gcttcgcccg tcaggatgcg gcgcgccagg gcctcggcgc gcgcggcctg
      481 cgcggcgtcc aggccaaagc ggcgtcgcgc ctgcagcagg cgccgcgcgt gccagccggc
      541 ggcgccgtcg ggcgcctgct ccagcagcca gtgcatggcc tcgcctacgc gggcgctgtc
      601 gctggaaggg ggttcggggc tgggttcagt agcttcgttt ttaatagctg ccggcgcttg
      661 ttgcgcgggc gccgcaggca gatcgagcat gatgaacgcg gcatcggcgg ggaccgtgcc
      721 ggcgacgccg gcgccgtcag ctgcgggcgc agccaggggt tcgccaagag gctcggccag
      781 cggctcgatg cgctgccacc agctgctggc cggctgggcg tgcggggtga tgccggacag
      841 caccagccgc tggcgcgcgc gcgtcatggc cacgtacagg gcgttgagct cctcgcgcgc
      901 ctgggcctgg tcttcctgct gcgccagtgc ggccaggctg ggcggtggct ggccgccctg
      961 ggccaggaac gccaggcagc gcggcgcggg ctcggcgccg cgccagtcga tcagcaccgc
     1021 ggcgccgttg ccgcgcggcg ggccggcggc ggcgtcgagc agcagcactt cgtcggcctc
     1081 cagccccttg gcgccatgca cggtgagcag ctgcaccaca cccggcgcgg cggcgcgcgg
     1141 caccggcagg cgctggcggc gcagcgcgcg cagccactgg taggcggtga gaaagcgccc
     1201 gccctgcagg ttcagggcgg ccgccagcag ggcgcgcagc tggatcagca cctgcgcccg
     1261 ctcgggcgcg ggggcagcgg cggcaaagcg cgccagcacg tcgcggtgct caaacatggc
     1321 ctgcagcgca tcgtgcggtg gcaacgtcag caagccttca cggtattgca tcaaatcggc
     1381 atgcagcgct tgtccatcgt gcgccgacag ctctgttatc gatagcactt cgagccagcc
     1441 ggggcgcggc tggccggcgc gctgcgcggc ctgggcggcc agcgccaggc gcgtcaggtc
     1501 gtcgtcgccg aggccgaaca ggggcgactt gagggcgcgc gccagcatca ggtcgtgacc
     1561 gggcgagagc agcgcgtcga ccagcgccag cacgtcctgc accgccggct gctggcccag
     1621 cagctgcttt tcgggctggt cgcaggcgat gcccaggcgg cgcagctcgg cctgcagcgg
     1681 gcccagcgca tcgcgcttgc gcgccagcac catgatctgc gccggcgcca cgccggtggc
     1741 gatgcggccg gcgatccagc gcgaggcctg gcggcattcg agcgtgcgca gggtttcgtc
     1801 gggctccagg cgcgggctga cgaggctgtc gcgccagggc gccggcgcgc cggcggcggt
     1861 ggccttgtcc gggcgcggca ccagcggcag cgcgcccagg gcgccgggcg cggtggcttg
     1921 ggtggtgtgg ggacgaaagc cgctgaactc gcccgccgcc tgcgcctgcc ccatgacgcc
     1981 gttgatggcc tgcagcagcg cgggggcgtt gcggtgggtg tggtcgcagg ccagcagttc
     2041 gccgcccagg gcggtgacga tgaagcgctg cgcgtcggcg aacacctgcg ggtcggcacg
     2101 gcgaaagcgg tagatgctct gcttggggtc gccgacgatg aacacgctgg gcgcgcccgg
     2161 cccgccgccg gcgccggcgt agcccgacag ccaggcgtgc agggcctgcc actgcagcgg
     2221 gttggtgtcc tgaaactcgt cgatcagcag atggcggatg cgcgcgtcca gccgctgctg
     2281 cacccaggcc gacagctcgt ggtcggccag cagggtgaag gcggcgcgct cgacgtcggg
     2341 catgtcgacc cagccgcgct cgcgcttgag ccgggccagg gcgtcgatca gcgggcgcag
     2401 caggcgcacc atgcgctggt ggtgctggtg ggcgtcgtgc tgggcggtgg ccggcagcag
     2461 gcgctgcagt tcggcctcgg cctgctgcgc ggcgccgtag gactgcaggt tcttggccag
     2521 gcggtcctcg ctggccacga agaacgcctt gcgcagggcg gccaggcgct ggcggccgtc
     2581 ggtcgccgcg tcaaaggcgt cgagcaccgc ggcggcggcc ttctgcggcg tcttgttctt
     2641 ttccgggccg agctggcgcg cgcgcgccag ccagcgctcg cgcgccgcct cggtcagcag
     2701 ggcgtcacaa gggtgggcga acggcgcgaa ctcggggtac agcgcgcccg ccggcgccac
     2761 cgccgcttca acatggccgg cggcgtcggc cagctggaac tccacccgcc gcgccagcgc
     2821 cgattccagc gcctggtgcg cacgctggcg gcccagggcg cccaccaggg cgtggtagtc
     2881 ggcgctcagg ccggcgtcgc cggccagctg gcgcagatag cggcgccaga cctcggccac
     2941 ggcgtcggca tcgtcttcca gcagctcgaa cgcggtcggc aggccaagct gctgcagcac
     3001 cgccagcggc gcgcacttga gcagcgccac aaaccagctg tgaaaggtgc ggatctgcac
     3061 cgggcggccg tggccgagca cggaatgata caaattttgt agctgctggc gctgatcggg
     3121 tgcggcttgc ggctcgattc ctcttgcaat cagtgcggct tgcaggcgct cgtcccaggc
     3181 gtcgggcgat tcgtccggcc gcgcgcgggc gaagtcggcc agccattcgt gcaggcgctg
     3241 gcgcatttcg ccggcggcct tcttggtaaa ggtgatggcc aggatctcgt gcggcgcgct
     3301 gcccgccagc agcgcgcgca gcatgcgcga caccagcatc caggtcttgc ccgcgccggc
     3361 gcaggcttcc accgccacgt ggcggcgcgg gtcgcaggcg atggcataga agcgctcgcg
     3421 cgagcagggc tggccgtggt gttcgtaggc aggggtcatg ggcgcgccag acattcaacc
     3481 tggaaacggc cgttggatgc gcctgcccgt gccgagatcg gtgtgccagg caagggtgag
     3541 cgccgcggct ccaaaggcgc ctgcgcgcct tgggacggcg cgaacgggcc cagcagcggt
     3601 gctggccggt gcctagcgcc ttcacccaga aggtgaaggc tcccaagtgg cgcaaggtca
     3661 gcgttcatgc ggcattccca agggttgcaa gcggtcaact gcggtttcca ggaggctgtc
     3721 gggcttggag cgccgaaagc gttctcgaag agcggcgaag ccaaaaatgg gccgctacgg
     3781 tcgatgtgca gccggcgatt tccaagtccg acagcctcct aggttcaaca cccgttcgca
     3841 gagtgcgcag cgatgacgcc gaagtcgcca aggcaatcca tggtccgtgc tgcgtgttct
     3901 gcgaaaccct ggcgtgctct gcgtggggag gttggcactt tgacatcaac cggcacccca
     3961 gaaatccctg cggcacaggc cgcgcgcggc gcaccagtcg catacccggc cttcgcccag
     4021 cgccggcagc gcggcgccgg cggcgatgcg ggcgacgtcg tgcgccatgc cgtcgtacag
     4081 ccgggcggcc agctcgggca gctcgggcaa ctccagcagg tgcggctgcg actcgcgctc
     4141 ggccaggttc aggtaggccg cgcgcggtgc gtcggcgccg gacagcaggg cgtagaaggg
     4201 cagctgggtc tcttcggagc cggcctggac gcgctggctg gatgtcttga gcgcttcggt
     4261 cttgtagtcg atcagcagac gggtgccgtc ggccaggcgg tcgatgcgat cgatgcgacc
     4321 ggtcagctgc agcgtgccgc agacgcgctg catgggttgc tcggcggcct cgaagcgcgc
     4381 gccgccggcc tcgtggtcgc gcagccagcc caggtaggcg tcgcgcagcg ccggccaggc
     4441 ggcggcaaag ggcatgaatt caccggtcgc ctggtccagc ccgagcgcct cggtggtgtg
     4501 ctcggccgcg gcgtcgatca gcgcccggcg gtcggcctgc ggcgcgccct gcagcgcctc
     4561 gtgaaacgcc cgcagcgtgg cgtgcagcca gttgccccag tcgcgcttgt ccagctcgac
     4621 gtccagctcg ccctcgtcct gcaggccaag ctggtgcagc gcgaagaagc ggtacgggca
     4681 ggcgcgcagc atgtcgtagc tgctggccga cagcggttgc agcggcagcg ccgcgccgct
     4741 gggcgccggg cgcgcgccgg gggcggcggc gatggggcgc ggcaggcgcg gctcggccgc
     4801 cgcgccgacg tcgagcgcgt gcccgcccag ttgcagcacc tgcaccaggg gcgaggcctg
     4861 cagcggctcg ccgctgtcgt cgccgcggcg ccacagcagg tccaccgccg gcacgcccag
     4921 ggcctgggcc caggtcgcgg cctgcgccca catcagcgcc tcgcgcgtcg gcaggcccag
     4981 cgcggcgcgc tgggcggcgc tccaggggcc gggcggttcg ggcgcggcgg gcagacgctg
     5041 ctcgtcggcg ccgggcagca ccagggcggc aaacggccgg cccggcaact gcgccagcgg
     5101 cagcaccacc acctgcgccg atcccgacgg cgacgggcga aagctggcgc cctccagggc
     5161 gtcggccacc cagcggctga actcggccag cccgacgcgg cgctgcgccg ccggccagtc
     5221 gcgccagtcg gccagcgcct catcgtccag ccccagggcg gccaccacgg cctgcccggc
     5281 cacgtcctgc accagcggcg cccacaggcc gcaaccctgc agcaacgcgc gcagggcggc
     5341 cagccagtcg cacacccggc gcgtgccgat cagcggtgcg cgcagcgcct cgacctgctg
     5401 cgtcagcggc tcgcccgacg tcaggcgcac cgcctgcgcc cagccgcgca cggcgtcgcg
     5461 gcgcaggcgg tgctccagcc ggcccagcgc ctgggggtcg atcacgggcg ccagcttgag
     5521 ccagtcgagc acctcgtcgc tgctggcctg cggtgcgcag gcgcgcagcg ccgccatcac
     5581 ccgcgcggcg gcgtggctgg tggacaggcg ccagccggtt tcgtcgcgca gcagcccgcc
     5641 ggccgtcacg ccacgcgccg ccagcagggc gtggatgcgc cgctgaagca cgcggtcggt
     5701 ggccaccagc gccaccggcg tgcggccggc ctgcaggtgc tgcagcacgc aggcggcggc
     5761 gcgtgcggcc tcgtcttcgc cgtcggcgca ggcgtgcagc gccacgcgcc cgcgtgcgac
     5821 gtccccggcc agcgacagcc gcaccagctt gtcgaccccc tgcgcggcca gggtggcggt
     5881 cagcgggtcg ggttgcagac cttcgacgat caccagcgca tccagcgcgc gcatcacacg
     5941 cggctcgaac agcacgtcgg tggcgtagtc ggaatgcgcc gcccaggcca gtgcaatgcg
     6001 gcccagcgcg ccttccagcc ccagcggacc gtcgccggcc ggtggcatgg cggcgcgcgc
     6061 gcgctcggcc caatcggggc gcagcgccgg cggcagaccg gccgccacct gcgccagctg
     6121 ctgggcctgc tccaccagcg gcgccgccag gccggcgcgc tgcgcgccca ggccggcgcc
     6181 ctgcagcagc gcggcggcgc tgagcaggtc gcgcccgcgg tcgaagctca ggtcgttggg
     6241 gccgggctcg aacatgccga cgcgcgcagc ccagctgcgc gtggtctcga agcgcggtgc
     6301 gaaaccgtcg ggatgctcgc gcgcccacca ggcggcggcc agcggcagca gctgcgcgaa
     6361 cggcagcagc accacgcagc gcgccgggtg cgccgccagc tggtgcaatt gctggcgcac
     6421 ctcggccagc agc
//
LOCUS       DAOQTZ010000131         6401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13359_length_6401_cov_14.054050, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000131 DAOQTZ010000000
VERSION     DAOQTZ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13359_length_6401_cov_14.054050"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKN51_12125"
     CDS             <1..531
                     /locus_tag="PKN51_12125"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNT85888.1"
                     /translation="AVAKITGLKNPVITGPARVFDDEQSALAAILSGKIKAGDVMVLR
                     YLGPKGGPGMPEMLAPTSALIGEGLGESVGLITDGRFSGGTWGMVVGHVAPEAFVGGT
                     IALVHEGDSITMDAHRLLLQLNVSDEELARRRARWTPPAPRYTRGVQAKFAFNAASAS
                     RGAVLDDFQSPPPMRR"
     gene            complement(596..850)
                     /locus_tag="PKN51_12130"
     CDS             complement(596..850)
                     /locus_tag="PKN51_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04438 family Trp-rich protein"
                     /protein_id="HNT85889.1"
                     /translation="MYLLGLGIILLLMKWQELGPVAAWSWWWVLSPFAGAALWWTWAD
                     WSGYTKRKAMEREDQRKLDRINRQRAAIGQKPKAAPPRRR"
     gene            958..1269
                     /locus_tag="PKN51_12135"
     CDS             958..1269
                     /locus_tag="PKN51_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNT85890.1"
                     /translation="MKRVLLTFAALTAVTVSAPALADEAMAKAKNCMACHATDKKLVG
                     PSYKDVAKKYASDAKAVDTLAAKIIKGGSGVWGAVPMPPNANVNDAEAKKLAAWVMSL
                     K"
     gene            complement(1374..3356)
                     /gene="acs"
                     /locus_tag="PKN51_12140"
     CDS             complement(1374..3356)
                     /gene="acs"
                     /locus_tag="PKN51_12140"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNT85891.1"
                     /translation="MSAIQSVLVENRVFPPSEAMVKAARISGMGAYQALYEEAEKDFE
                     GFWARRAREHLVWAKPFTQVLDESNKPFYKWFADGQLNASANCLDKHMGTPVENKTAI
                     IFEADGGEVTKVTYKELLARVSQFANGLKAHGIRKGDRVLVYMPMTIEGLIAMQACAR
                     IGATHSVVFGGFSAKAVQERIQDAGAVAVITANYQMRGGKELPLKAIVDEALSLGGCE
                     SIKNVFVYERTQTPCNMVAGRDKTFAQMLQGQSTECAPEIVDAEHPLFVLYTSGSTGK
                     PKGVQHSTGGYLLWAKMTMEWTFDLKDSDVFWCTADIGWVTGHTYIVYGPLAAGATQI
                     MFEGVPTYPDAGRFWQMIERHQCSIFYTAPTAIRSLIKASETNDKVHPRNWNLSSLRI
                     LGSVGEPINPEAWMWYYRNVGGERCPIVDTWWQTENGGHLITPLPGATPLVPGSCTLP
                     LPGVFAGIVDEAGNDMPWGSGGILVIKKPWPSMIRTIWGDPARFKSSYFPEELKGYYL
                     AGDGAVRDAERGYFRVTGRIDDVLNVSGHRMGTMEIESALVAKTDLVAEAAVVGRPDE
                     TTGEAICAFVVLKRPLPQGDEAKQIAKELRDWVAKEIGPIAKPKDIRFGENLPKTRSG
                     KIMRRLLRSLAKGEQITQDTTTLENPAILAQLDQTY"
     gene            complement(3581..4975)
                     /gene="fumC"
                     /locus_tag="PKN51_12145"
     CDS             complement(3581..4975)
                     /gene="fumC"
                     /locus_tag="PKN51_12145"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNT85892.1"
                     /translation="MSDADKTTRVERDSFGPIEVPAERLWGAQTQRSLRHFAISGERQ
                     PRELIHALTQVKRAAAVVNQRLGQLDATLAGAIVRAADEVLAGRHDDEFPLVVWQTGS
                     GTQTNMNLNEVLANRASELLGGPRGEQRLVHPNDHVNRSQSSNDAFPTAMHVAAAEAI
                     ERRLLPALTRLRATLAGKSRDFADIVKIGRTHLQDATPLTLGQELSGWVAQLAHAGRH
                     VRAALPHLHELALGGTAVGTGLNAPEGYAEAVAQELARLTGLPFVTAPNKFEALASCD
                     ALVQAHGTLKGLAASLTKIANDVRWLASGPRSGLGEISIPENEPGSSIMPGKVNPTQC
                     EALTMLCAQVMGNDVAISIGGASGHFELNVFRPMIAHAFLQSARLLADGMNSFNDHCA
                     VGIEPNRARIDELVQRSLMLVTALNPHIGYDKAAQIAKRAHQEGLSLREAALATGWLS
                     AAQFDAWVRPQDMV"
     gene            5088..>6401
                     /locus_tag="PKN51_12150"
     CDS             5088..>6401
                     /locus_tag="PKN51_12150"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNT85893.1"
                     /translation="MTTIIKQGDLIESIAAALQYISYYHPADYIQHLARAYEREQSPA
                     AKDAMAQILTNSKMSATGHRPICQDTGIVNVFLKIGMNVRFEGFAVDQSLEDAVNEGV
                     RRGYNHPDNTLRASVVADPLFARKNTRDNTPAVINVQIVPGDKLDVTVAAKGGGSENK
                     SKMVMMNPSDNLVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLLAKESLMEDLNM
                     YELQAKAARGEKLDQVEELRLELYEKVNALGIGAQGLGGLSTVLDVKIKMYPTHAASK
                     PVAMIPNCAATRHAHFVMDGSGPVYLEAPSLQLWPKIDWAPDYTQSKRVQLDTLTKEE
                     VASWKPGDRLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVRDE
                     VVGPAGPTTATRMDKFTRMMLEQTGLIAMIGKSERGPVAIEAIKDN"
BASE COUNT         1078 a         2121 c         2127 g         1075 t
ORIGIN      
        1 gccgtggcca agatcaccgg cctcaagaac ccggtcatca ccggcccggc gcgcgtgttc
       61 gacgacgagc agtcggccct tgccgccatc ctgtcgggca agatcaaggc cggcgacgtg
      121 atggtgctgc gctacctcgg ccccaagggt gggcccggca tgcccgagat gttggcaccc
      181 acgagtgcac tcatcggcga gggcctgggc gagagcgtgg gcctgatcac cgacgggcgc
      241 ttttccggcg gcacctgggg catggtcgtg ggccatgtgg cgcccgaggc ctttgtcggc
      301 ggcaccatcg ccctggtgca cgagggcgac agcatcacca tggacgccca ccggctgctg
      361 ctgcagctca acgtgagcga cgaagagctg gcgcgcaggc gcgcgcgctg gacgccgccc
      421 gcgccgcgtt acacgcgtgg cgtgcaggcc aagtttgcgt tcaacgcggc gagcgccagc
      481 cggggagcgg tgctggacga tttccagagc ccgccaccaa tgcgccgata gctatcattc
      541 aaataactgc cgacgctttc ctggtgggcg gcagaggcca aatgggcata gatgttcagc
      601 ggcggcgcgg cggcgcggcc ttgggcttct gaccaatcgc cgcgcgctgg cggttgatgc
      661 ggtcgagctt gcgctgatcc tcgcgctcca tcgccttgcg cttggtgtag cccgaccagt
      721 cggcccaggt ccaccacagc gccgcaccgg caaacggcga cagcacccac caccaggacc
      781 aggcagccac cggcccgagt tcctgccatt tcatcagcaa cagaatgatt cccaaaccca
      841 gcagatacat ggttttcctt gatgtgttcc aggtttcatc ggtgcccggc gtcaaccgga
      901 aactttacaa tcgcgttcac tcactgtagc gcaaccaccc tcgatggaga tatgacgatg
      961 aagcgtgttc tgctcacttt tgccgctttg accgctgtga cggtgtcggc gcctgccctg
     1021 gccgacgaag ccatggccaa ggccaagaac tgcatggcct gccacgccac cgacaagaag
     1081 ctggtcggcc cctcctacaa ggacgtggcc aagaagtacg ccagtgacgc caaggccgtg
     1141 gataccctgg ctgccaagat catcaagggc ggctcgggcg tgtggggcgc ggtgcccatg
     1201 cctcccaacg ccaacgtgaa cgacgccgag gccaagaagc tggccgcctg ggtcatgtcg
     1261 ctgaagtaat cacgcgacat cccgcaacaa aaagcccgcc tgattcaggc gggctttttt
     1321 ttgtgcctgg ggccgagcgg cggcccgcgg ggccgccgtc gagcagctca ggatcagtag
     1381 gtctgatcga gctgcgccag gatggccggg ttttccagcg tggtggtgtc ctgcgtgatc
     1441 tgctcgccct tggccaggct gcgcagcagg cgacgcatga tcttgcccga gcgcgtcttg
     1501 ggcaggttct cgccgaagcg gatgtccttg ggcttggcga tcgggccgat ttccttggcc
     1561 acccagtcgc gcagttcctt ggcgatctgc ttggcttcgt cgccctgcgg cagcggacgc
     1621 ttgagcacca cgaaggcgca gatggcctcg ccggtggttt cgtcggggcg gccgaccacg
     1681 gcggcttcgg ccaccaggtc ggtcttggcc accagggcgg attcgatttc catggtgccc
     1741 atgcggtggc ccgagacgtt cagcacgtcg tcgatgcggc cggtgacgcg gaagtagccg
     1801 cgctcggcat cacgcaccgc tccgtcgccg gccaggtagt aacccttgag ctcctcggga
     1861 aaatagctgg acttgaagcg cgccggatcg ccccagatgg tgcggatcat gctgggccag
     1921 ggcttcttga tgaccaggat gccgcccgag ccccagggca tgtcgttgcc cgcctcgtcc
     1981 acgatgccgg caaacacgcc gggcagcggc aaagtgcagg aacccggcac cagcggcgtg
     2041 gcacccggca gcggggtgat caggtggccg ccgttctcgg tctgccacca ggtgtcgacg
     2101 atcgggcagc gttcgccgcc gacgttgcgg tagtaccaca tccaggcttc cgggttgatc
     2161 ggctcgccca cgctgcccag gatgcgcaac gaagacaggt tccagttcct gggatgcacc
     2221 ttgtcgttgg tttccgacgc cttgatgagc gaacggatgg ccgtgggcgc ggtgtagaag
     2281 atcgagcact ggtggcgctc gatcatctgc cagaagcggc cggcgtccgg gtaggtcggc
     2341 acgccctcga acatgatctg ggtggcgccg gcggccagtg ggccgtagac gatgtaggtg
     2401 tggccggtga cccagccgat gtcggcggtg caccagaaga cgtcgctgtc cttgaggtcg
     2461 aaggtccact ccatggtcat cttcgcccac agcaggtagc cgccggtgga atgctgcacg
     2521 cccttgggct tgccggtgga gccgctggtg tagagcacga acagcgggtg ctcggcatcg
     2581 acgatctcgg gcgcgcactc ggtgctctgc ccttgcagca tctgggcgaa ggtcttgtcg
     2641 cggccggcca ccatgttgca gggggtctgg gtgcgctcgt agacaaacac gttcttgatc
     2701 gactcgcagc cgcccaggct cagcgcctcg tcgacgatgg ccttcagcgg cagctccttg
     2761 ccaccgcgca tctggtagtt ggcggtgatg acggccacgg cgccggcgtc ctggatgcgt
     2821 tcctgcaccg ccttggccga gaagccgccg aacaccacac tgtgcgtggc gccgatgcgg
     2881 gcgcaggcct gcatcgcgat caggccctcg atggtcatcg gcatgtagac cagcacccgg
     2941 tcgcccttgc ggatgccatg ggccttcagg ccgttggcga actggctcac gcgcgccagc
     3001 agctccttgt aggtgacctt ggtgacttca ccgccgtcgg cttcgaagat gatcgcggtc
     3061 ttgttttcga ccggcgtgcc catgtgcttg tccagacagt tggcgctggc gttgagctgg
     3121 ccgtcggcaa accacttgta gaagggcttg ttggattcat ccagcacctg ggtgaagggt
     3181 ttggcccaca ccaggtgttc acgcgcacgc cgcgcccaga aaccttcgaa atccttctcg
     3241 gcctcttcgt acagggcctg ataggcgccc atgccgctga tgcgggccgc cttgaccatg
     3301 gcttcgctgg gggggaacac gcggttttcg accagaaccg attgaatggc gctcatgtgc
     3361 cttgtctcct ggataagagg gggaaagttg aacacacccg tcccgcgctg gcgggacaga
     3421 cggtttcatc atccggtcag gctcttacaa agcactgact ctgggcgggc ccgggcagcc
     3481 ggggctacgg cgcttggggc gcccgtcgat cttgggaatg aaattcggtt ccagcgcata
     3541 cgtggcaagc gccgctagct atgtttgtaa tagcattcga tcacaccatg tcctgcggcc
     3601 ggacccaggc gtcgaactgc gccgcactca gccagccggt ggccagggcc gcctcgcgca
     3661 ggctcaggcc ctcctggtgc gcgcgcttgg cgatttgcgc cgccttgtcg tagccgatgt
     3721 ggggattgag cgccgtcacc agcatcagcg agcgctgcac cagctcgtcg atgcgcgcgc
     3781 ggttgggctc gatgcccacc gcgcagtggt cgttgaagct gttcatgccg tcggccagca
     3841 gccttgcgct ctgcagaaag gcgtgggcga tcatggggcg aaacacgttc agctcgaaat
     3901 ggcccgacgc accgccgatg ctgatggcca cgtcgttgcc catgacctgc gcgcacagca
     3961 tggtcagggc ctcgcactgg gtcgggttga ccttgcccgg catgatggat gagccgggct
     4021 cgttctcggg gatgctgatc tcgcccaggc cgctgcgcgg gccgctggcc agccagcgca
     4081 cgtcgttggc gatcttcgtc aggctggcgg ccaggccctt gagcgtgccg tgcgcctgca
     4141 ccagggcgtc gcagctggcc agggcctcga acttgttggg cgcggtcacg aagggcagcc
     4201 cggtcaggcg cgccagctcc tgcgccaccg cctcggcgta gccttcgggc gcgttcagac
     4261 cggtgccgac ggccgtgccg cccagcgcca gttcgtgcag atgcggcagc gccgcgcgca
     4321 catggcgccc ggcatgggcc agctgcgcca cccagcccga cagctcctga ccgagcgtga
     4381 gcggtgtggc gtcctgcagg tgggtgcggc cgatcttgac gatgtcggca aagtcgcgcg
     4441 acttgcccgc cagcgtggcg cgcaggcgcg tcagggcggg cagcaaccgc cgctcgatcg
     4501 cctcggccgc agccacgtgc atggcggtcg ggaaggcgtc gttgctggac tggctgcggt
     4561 tgacgtggtc gttcggatgc accaggcgct gctcgccgcg cgggccgccc agcagctcgc
     4621 tggcgcggtt ggccagcacc tcgttgaggt tcatgttggt ctgcgtgccg gagccggtct
     4681 gccagaccac cagcgggaat tcgtcgtcat gccggccagc cagcacttcg tcggcagcac
     4741 gcacgatggc gcccgccagc gtcgcatcga gctgacccag gcgctggttg accaccgccg
     4801 ccgcccgctt gacctgggtc agcgcgtgaa tgagctcgcg tggctggcgt tcaccgctga
     4861 tggcgaagtg gcgcagcgag cgctgcgtct gcgcccccca caggcgctcg gcgggcacct
     4921 cgatcgggcc aaagctgtca cgctccacgc gcgtggtctt gtcagcatcg ctcatgggtc
     4981 ggcctgtcaa aatacgcgga tacggtgcac ggtagcagaa cggcaatgcc cctgtggcaa
     5041 ttgccggcgt gcccatgcgt tttccagact tccccgagga tgcccccatg accacgatca
     5101 tcaaacaagg cgacctgatc gaatccatcg ccgccgcgtt gcagtacatc agctactacc
     5161 accccgccga ctacatccag cacctggcgc gcgcctatga gcgcgagcaa agccccgcgg
     5221 ccaaggacgc gatggcacag atcctcacca acagcaagat gagcgccacg ggccatcgtc
     5281 cgatctgcca ggacacgggc atcgtcaacg tgttcctgaa gatcggcatg aacgtgcgct
     5341 tcgagggctt tgccgtcgac cagagcctgg aggacgccgt caacgaaggc gtgcgccgcg
     5401 gctacaacca cccggacaac acgctgcgcg ccagcgtggt ggccgacccg ctgttcgcac
     5461 gcaagaacac ccgcgacaac acccccgccg tcatcaacgt gcagatcgtg ccgggcgaca
     5521 agctcgacgt gaccgtggcc gccaagggcg gcggcagcga gaacaagtcc aagatggtca
     5581 tgatgaaccc gagcgacaac ctggtcgact gggtcttgaa gaccgtgccc accatgggcg
     5641 cgggctggtg cccgccgggc atgctgggca taggcatcgg cggcacggcg gaaaaggccg
     5701 tgctgctggc caaggaaagc ctgatggaag acctgaacat gtacgagctg caggccaagg
     5761 ccgcgcgcgg cgagaagctc gaccaggtcg aggagctgcg cctggagctc tacgagaagg
     5821 tcaacgcgct gggcattggc gcccagggcc tgggcggcct gtccaccgtg ctggacgtga
     5881 agatcaagat gtaccccacg catgccgcga gcaagcccgt ggccatgatc cccaactgcg
     5941 cggccacgcg ccacgcgcat ttcgtcatgg acggcagcgg ccccgtgtac ctggaggccc
     6001 cgagccttca gctatggccc aagatcgact gggcgccgga ctacacccag tccaagcgcg
     6061 ttcaactcga caccctgacc aaggaagaag ttgcgagctg gaaacccggc gaccgcctgc
     6121 tcttgaacgg caagatgctc accggccgcg acgccgcgca caagcgcatc caggacatgc
     6181 tggccaaggg cgagaagctg cccgtggact tcaccaaccg cgtgatctac tacgtcggcc
     6241 ccgtggaccc ggtgcgtgac gaggtcgtcg gccccgccgg ccccaccacg gccacgcgca
     6301 tggacaagtt cacgcgcatg atgctcgagc agaccggcct gatcgccatg atcggcaagt
     6361 ccgagcgcgg cccggtcgcc atcgaggcca tcaaggacaa c
//
LOCUS       DAOQTZ010000132         6394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13381_length_6394_cov_13.171320, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000132 DAOQTZ010000000
VERSION     DAOQTZ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13381_length_6394_cov_13.171320"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKN51_12155"
     CDS             <1..197
                     /locus_tag="PKN51_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNT85894.1"
                     /translation="LRNEKITYKIREHSLQKLPYILVVGDKEREAGAVAVRARGNVDL
                     GVMSLEAFAKRIGEDIASKA"
     gene            283..888
                     /gene="infC"
                     /locus_tag="PKN51_12160"
     CDS             283..888
                     /gene="infC"
                     /locus_tag="PKN51_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNT85895.1"
                     /translation="MATNFRDRRQREERAHRLNREIMAPEVRLNGPNNEPLGIVPLQE
                     ALRMAGDLDVDLVEVVATANPPVCRLMDYGKFKYQEQKKAAEAKAKQTVIEIKEVKFR
                     PGTDDGDYQIKMRNIRRFLADGDKVKVTLRFRGREITHQELGLALLDRIRADLADSVV
                     VEQFPKLEGRQMIMMLAPARKKTGGGAATKAAGADQQPAAA"
     gene            1043..1246
                     /gene="rpmI"
                     /locus_tag="PKN51_12165"
     CDS             1043..1246
                     /gene="rpmI"
                     /locus_tag="PKN51_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNT85896.1"
                     /translation="MPKMKTKSSAKKRFRVRPGGTVKRGQAFKRHILTKKTTKNKRQL
                     RGAVNVHETNLGHMAQMLPFAGL"
     gene            1273..1629
                     /gene="rplT"
                     /locus_tag="PKN51_12170"
     CDS             1273..1629
                     /gene="rplT"
                     /locus_tag="PKN51_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNT85897.1"
                     /translation="MPRVKRGVTARARHKKVLALAKGFRGRRKNVFRVAKQAVMKAGQ
                     YAYRDRRNKKRDFRRLWIARINAAARECGITYSQFINGLSKAGIALDRKVLADIAVHD
                     KAAFAGIVEQAKAKLA"
     gene            1749..2816
                     /gene="pheS"
                     /locus_tag="PKN51_12175"
     CDS             1749..2816
                     /gene="pheS"
                     /locus_tag="PKN51_12175"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNT85898.1"
                     /translation="MTTTATEDLQELVRSADSSFARARTPAELENAKAQFLGKSGRVT
                     ELMKGMGALSVDEKKTRGAAINQAKQAIEALLTDRRQALADAELARQLQAEALDVTLP
                     GRQRGMGALHPISLAWERIEQIFGSMGFDVGDGPEIENDWFNFTALNNPPNHPARSMQ
                     DTFYVDMSDEQGQPYCLRTHTSPMQIRYATAHARRHAAALAAGQIPEVRVIVPGRTYR
                     VDNDATHSPMFHQVEGLWLGENVSFKDLKVIYLNFCKAFFETEDLILRFRPSYFPFTE
                     PSAEIDIQFQTGPLAGRWLEVSGSGQVHPQVVRNMGLDPERFIGFAFGSGIDRLAMLR
                     YGVNDLRLFFEGDIRFLSQFR"
     gene            2875..5310
                     /gene="pheT"
                     /locus_tag="PKN51_12180"
     CDS             2875..5310
                     /gene="pheT"
                     /locus_tag="PKN51_12180"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNT85899.1"
                     /translation="MQFPESWLREFCNPPLSTEQLADTLTMAGFEVEELRPVAPPFTR
                     VVVAEIRSAEPHPNADRLRVCQVDAGQGTLLNIVCGAPNARAGIKVPCALVGAELPPG
                     EDGKPFLIKTGKLRGVESQGMLCSARELQLSDDHGGLLELPADTPVGQDIRAALRLDD
                     AVFTLKLTPNLGHALSVYGIARELSALTGAPLRTPTLPPVTVGCDQVLPVRVSAPDLC
                     GRFSGRVIRHVNTRAATPPWMVDRLARCGQRSVTALVDISNYVMFEYGRPTHIFDLDR
                     IHGGLDVRWGRAGETLKLLNGNTIEVDETVGVIADEREVESLAGIMGGDATAVSDDTQ
                     HVYVEAAFWWPDAVAGRSRRFNFATDAGHRFERGVDPALTVAHIERISQLIVEICGTP
                     QTVCGPIDDQQPQLPVPAPVTLRVARAAQIIGMPLTAAQCADVFGRLGLPFTEGQGTI
                     TVVPPSYRFDLRIEEDLIEEVARVIGYQQLPDTAPLAPIVPRVPSATRRGAFAVRRAL
                     AGLGYLETINFSFVDESWEHQLCGNPDPVRLLNPIASQMSVMRSSLLGSLLQVIKHNV
                     DRRAERVRVFELGRVFRRDATVATSDADVRGVAQPMHVAAAALGPTSRQGWQGRQGAV
                     DFYDVKGDVEALLSPRQPTFEPAEHPALHPGRSARVLLDGRPIGVIGELHPRWRQQWE
                     LPQAPILFELALDAVMQQALPQAQPVPRQQPVERDIAVVVPESVTHAALTQAIHAAPT
                     EGLLRSATLFDIYRPKPGAEGQAGMASHEKSLAVRLVLGSTHDTLTDERIDAAVKAVL
                     ERLATELGARLRA"
     gene            5364..5729
                     /locus_tag="PKN51_12185"
     CDS             5364..5729
                     /locus_tag="PKN51_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNT85900.1"
                     /translation="MLLAVDSLETSALTKAQLAELLFEQIGLNKREAKDMVDAFFELI
                     VQHLIAGDEVKLSGFGNFQMRVKAPRPGRNPRTGELIPIAARRVVTFHASQKLKDQLQ
                     AEAGSPAPLQVGAEPDQAA"
     gene            5833..6303
                     /locus_tag="PKN51_12190"
     CDS             5833..6303
                     /locus_tag="PKN51_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNT85901.1"
                     /translation="MDKTLPPIPAKRYFTIGEVGELCGVKPHVLRYWEQEFTQLRPMK
                     RRGNRRYYQHHEVLMIRRIRELLYDQGFTINGARVKLQELTQHERDRARAGELALHGL
                     DRFDADETVPSEFGDSLSFEDSQLMPEQIREVERELKEIRDLLTVSQWHDLHAL"
     gene            6316..6392
                     /locus_tag="PKN51_12195"
     tRNA            6316..6392
                     /locus_tag="PKN51_12195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6350..6352,aa:Pro,seq:ggg)
BASE COUNT         1159 a         2153 c         2065 g         1017 t
ORIGIN      
        1 acctgcgcaa cgagaagatc acgtataaaa tacgcgagca ttcgttgcaa aagcttccct
       61 acatcctggt cgtgggcgac aaggagcggg aagccggtgc cgttgcggtg cgtgcccgag
      121 gcaatgtgga cctcggcgtg atgtctctcg aagcctttgc caagcgcatc ggcgaagaca
      181 tcgcttccaa agcctgattt ctgaccgaaa gcagctctgg ccagcatgca aggcgcggcg
      241 ttcgctattt gttcgatagc atactgaact gaggtttcaa ccatcgctac caattttcgt
      301 gaccgccgcc aacgtgaaga gcgcgcgcat cgtctgaacc gagaaatcat ggcgcccgaa
      361 gtgcgcctga atggccccaa caacgaaccc ctggggatcg tgccactgca ggaagccctg
      421 cgcatggcgg gcgatctgga tgtggatctg gtcgaagtgg tggccacggc caacccgccg
      481 gtctgccgcc tgatggacta cggcaagttc aagtaccagg agcagaagaa ggccgccgag
      541 gccaaggcca agcagacggt catcgagatc aaggaagtca agttccgccc cggtaccgac
      601 gatggcgatt accagatcaa gatgcgcaac attcgccgct ttctggccga tggcgacaag
      661 gtcaaggtta cgctgcgctt tcgcggccgc gaaatcaccc accaggaact ggggctggcg
      721 ctgctcgatc gcatccgtgc cgatctggcc gacagcgtgg tggtggagca gtttcccaag
      781 ctcgaagggc gccagatgat catgatgctg gcacctgcgc gcaagaagac gggtggcgga
      841 gcggccacca aggcggcggg cgcggaccag cagccagcag ccgcctgaag ggcgacgcag
      901 acgatcgaac ttgaatttgc cgggttcgcc cggtgaaccg gcaggctgca aggcctgcca
      961 gcagaagtgg ctcggggcca acaagggtgc aaagtcgttt tgcacacgcc tcgcgagcac
     1021 aaagccaaca ggagcatgtc acatgcccaa aatgaagacc aagagcagcg cgaagaagcg
     1081 ttttcgcgtc cgtcccggtg gcaccgtcaa gcgcgggcaa gccttcaagc gtcacatcct
     1141 gaccaagaag accaccaaga acaagcgcca gttgcgtggt gcggtgaatg tgcatgagac
     1201 caacctcggt cacatggcgc agatgctgcc ctttgctggc ctgtgatcaa ctgacggaca
     1261 aggagaacac acatgcctcg cgtcaaacgt ggtgtgaccg cccgcgcgcg tcacaagaaa
     1321 gtgctggccc tggccaaggg tttccgcggc cgccgcaaga acgtcttccg cgtcgccaaa
     1381 caggcggtga tgaaggccgg gcagtacgcc taccgtgacc gccgcaacaa gaagcgcgat
     1441 ttccgccgcc tctggattgc ccgcatcaac gccgccgcgc gtgaatgcgg catcacctac
     1501 agccagttca tcaacggcct gagcaaggcc ggcattgcgc tggatcgcaa ggtgctggcc
     1561 gacatcgccg tgcacgacaa ggccgccttt gccggcatcg tggagcaggc caaggccaag
     1621 ctggcctgat tccgacgccg gtgcagcaac tatacttttg atagctgctc gcgctggcgg
     1681 ttcaaggggt tgaggccttt ttggcttcaa tccctttttc atttccgttt cactgcccga
     1741 ttgtttcgat gaccaccacc gccaccgagg acctgcagga actcgtccgc agcgccgaca
     1801 gcagctttgc ccgcgcccgt acgccggccg aactcgaaaa cgccaaggcc cagtttcttg
     1861 gcaagtcggg gcgcgtgacc gaactgatga agggcatggg tgcgctgagc gtcgacgaga
     1921 agaagacccg cggtgccgcc atcaaccagg ccaagcaggc catcgaagcg ttgctgaccg
     1981 atcgccgtca ggcgctggcc gacgccgagc tggcgcgcca gctgcaggcc gaggcgctgg
     2041 acgtgacgct gcccggccgc cagcggggca tgggcgcgct gcaccccatc agcctggcct
     2101 gggagcgcat cgagcagatc ttcggcagca tgggcttcga cgtcggcgac gggccggaga
     2161 tcgagaacga ctggttcaac ttcaccgccc tgaacaaccc gcccaaccac cccgcgcgct
     2221 cgatgcagga caccttctac gtcgacatga gcgacgagca gggtcagccc tactgcctgc
     2281 gcacccacac cagcccgatg cagatccgct acgccacggc gcacgcgcgc cggcacgccg
     2341 cagcgctggc ggcgggccag atccccgaag tgcgcgtgat cgtgcctggc cgcacctatc
     2401 gggtcgacaa cgatgccacc cattcgccca tgttccacca ggtcgaaggc ctgtggctgg
     2461 gggagaacgt cagcttcaag gacctgaagg tcatctacct caacttctgc aaggccttct
     2521 tcgaaaccga agatctgatc ctgcgttttc ggcccagcta tttcccgttc accgagccga
     2581 gcgccgagat cgacatccag ttccagaccg gcccgctggc gggccgctgg ctggaagtgt
     2641 ccggctccgg ccaggtgcac ccgcaggtgg tgcgcaacat ggggctggat ccggagcgct
     2701 tcatcggctt tgccttcggc tccggcatcg atcgtctggc catgctgcgc tacggcgtca
     2761 atgacctgcg cctgttcttc gaaggcgaca tccgcttcct gagccagttc cgctgattca
     2821 ggcgcagcct cgttcaccgg cccacgtccc acctgctcac ccctccaaag aaaaatgcag
     2881 tttcccgaat cctggttgcg cgagttctgc aacccgcctc tttccaccga gcagctggcc
     2941 gacaccctga ccatggccgg cttcgaggtg gaggagctgc gccccgtggc gccgccgttc
     3001 acgcgcgtgg tggtggccga aatccgcagc gccgagccgc accccaacgc cgaccgcctg
     3061 cgcgtgtgcc aggtcgacgc cgggcagggc acgctgctga acatcgtgtg cggcgcgccc
     3121 aacgcgcgcg ccggcatcaa ggtgccctgt gccctggtgg gcgccgagct gccgccgggc
     3181 gaggacggca agccttttct catcaagacc ggcaagctgc gcggcgtcga gagccagggc
     3241 atgctgtgtt cggcgcgtga gctgcagctg tcggacgacc atggcggcct gctggagctg
     3301 cccgccgaca cgccggtggg gcaggacatc cgcgccgcgc tgcggctcga cgacgccgtg
     3361 ttcacgctca agctcacccc caacctgggc cacgccctga gcgtgtacgg catcgcgcgc
     3421 gaactgtcgg ccctgacggg cgcgcccttg cgcacgccga cgctgccgcc ggtgaccgtg
     3481 ggctgcgatc aggtgttgcc ggtgcgcgtc agcgcgcccg acctgtgcgg ccgcttttcc
     3541 ggccgcgtga tccgccacgt caacactcgc gccgccacgc cgccgtggat ggtcgatcgg
     3601 ctggcgcgct gcggtcagcg cagcgtcacc gcgctggtgg acatctcgaa ctacgtgatg
     3661 ttcgagtacg gacggcccac ccacatcttc gatctggacc ggatccacgg cggcctggac
     3721 gtgcgttggg gccgtgccgg cgaaaccctg aaactgctca acggcaacac catcgaggtg
     3781 gacgaaaccg tcggtgtgat cgctgacgag cgcgaggtcg agtcgctggc gggcatcatg
     3841 ggcggcgacg ccaccgcggt ctccgacgac acgcagcatg tctacgtcga ggccgccttc
     3901 tggtggcccg acgccgtggc cgggcgttcg cgccgcttca acttcgccac cgatgccggg
     3961 caccgtttcg aacgtggggt cgacccggcc ctgacggtgg cgcacatcga acgcatcagc
     4021 cagctcatcg tcgagatctg cggtacgccg cagaccgtgt gcggcccgat cgacgaccag
     4081 cagccccagc tgccggtgcc ggcgccggtc acgctgcggg tggcgcgcgc ggcgcagatc
     4141 atcggcatgc cgctgacggc cgcacagtgc gccgacgtgt tcggtcgtct gggcctgccg
     4201 ttcaccgaag ggcagggcac gatcaccgtg gtgccgcctt cctaccggtt cgacctgcgc
     4261 atcgaggaag acctgatcga ggaagtggcg cgcgtgatcg gctaccagca actgccggac
     4321 accgcgcctc tggcgccgat cgtgccgcgc gtgccctcgg ccacccgacg cggcgccttt
     4381 gccgtgcgcc gcgcgctggc gggtctgggt tacctggaaa ccatcaactt cagcttcgtc
     4441 gacgaaagct gggagcacca gctgtgcggc aatcccgatc cggtgcgcct gctcaacccc
     4501 atcgccagcc agatgagcgt gatgcgctca tcgctgctgg ggtcgctgct gcaggtcatc
     4561 aagcacaacg tcgaccggcg cgccgagcgc gtgcgtgtgt tcgagctggg gcgcgtgttc
     4621 cggcgtgacg ccacggtggc caccagcgac gccgacgtgc gcggcgtggc ccagcccatg
     4681 cacgtggcgg cagccgcgtt ggggcccacc agccgccagg gttggcaggg ccggcaggga
     4741 gcggtggatt tctacgacgt caagggtgat gtcgaagccc tgctcagccc gcgccagccc
     4801 acgttcgagc ccgccgagca tccggccctg cacccggggc gcagcgcgcg cgtgctgctg
     4861 gacgggcggc cgatcggcgt catcggcgag ctgcacccac gctggcgcca gcagtgggag
     4921 ctgccgcagg cgccgatcct gttcgagctg gcgctcgacg ccgtgatgca gcaggcgctg
     4981 ccccaggcgc agcccgtgcc gcgccagcag ccggtggagc gcgacatcgc cgtggtggtg
     5041 cccgaatcgg tcacccatgc ggctctgacg caggccatcc atgctgcccc caccgagggc
     5101 ctgctgcgca gcgccacgct gttcgacatc tatcgcccga aaccgggcgc ggaagggcag
     5161 gccggcatgg ccagccatga aaaaagcctg gccgtgcgcc tggtgctggg cagcacacac
     5221 gacacgctga ccgacgagcg catcgacgcc gccgtgaagg cggtgctgga gcggctggcg
     5281 accgagcttg gcgccaggct gcgcgcctga tgctcaccga tggagagacg cccatggccg
     5341 ctcacgatcc gaccgaagac gccatgctgc tggccgtcga cagcctggaa acctcagccc
     5401 tgaccaaggc tcagctggcg gaactgctgt tcgagcagat cggcctgaac aagcgcgaag
     5461 ccaaggacat ggtcgacgca tttttcgaac tcatcgtgca gcacctgatc gcgggcgacg
     5521 aggtcaagct gtcgggattt ggcaatttcc agatgcgcgt caaggcgcca cgtccgggcc
     5581 gcaacccgcg caccggcgaa ctcattccca tcgccgcccg tcgggttgtc acgtttcacg
     5641 ccagccagaa gctcaaggac cagctgcagg ccgaggccgg gtccccggca ccgctgcagg
     5701 ttggggccga acccgaccag gccgcctgac cccctgtttc agggggtgtc ggggtggggt
     5761 gtcgctggcc tgtagccgcg acgaacgagt tgcggtaacc tcactggttc agcacgcaag
     5821 tgcctgatcc gcatggacaa gaccctgcct ccgattcctg ccaagcgcta cttcaccatc
     5881 ggcgaagtcg gcgagctgtg cggcgtcaaa ccgcatgtgc tgcgctattg ggagcaggaa
     5941 ttcacccaac tgcggcccat gaagcgccgt ggcaaccgac gctactacca gcaccacgag
     6001 gtgctgatga tccggcgcat ccgcgagctg ctgtatgacc agggcttcac catcaatggc
     6061 gcgcgggtga aactgcagga attgacccag cacgagcgcg accgtgcccg ggccggcgaa
     6121 ctggctctgc acggtctcga ccgtttcgac gccgacgaaa ccgtgcccag tgagtttggt
     6181 gacagcctgt cgttcgagga ctcccagctc atgcccgaac agatccgcga agtcgaacgc
     6241 gaactgaagg aaatccgcga tctgctcacc gtctcgcagt ggcacgattt gcacgcgcta
     6301 taatgcaagg cttgtcggcg tgtagcgcag cctggtagcg cacttgcatg gggtgcaagg
     6361 ggtcgcgagt tcgaatcccg ccacgccgac caat
//
LOCUS       DAOQTZ010000133         6387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13397_length_6387_cov_13.384397, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000133 DAOQTZ010000000
VERSION     DAOQTZ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13397_length_6387_cov_13.384397"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..762)
                     /locus_tag="PKN51_12200"
     CDS             complement(37..762)
                     /locus_tag="PKN51_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT85902.1"
                     /translation="MSRLCPQCGAENRDAAKFCLKCAHQLVPLSEPTQPLPLEQPARK
                     RRRRKRPAEAPPRKRHAWLWGLVALVAVVALGGALWRGDTRPDTAPPQAAATPAGAAA
                     SSSPAAVEPASPASAASAAPPPLPAASSAPIPSTSSAAPAAPTRPSTAARRSKPSPPA
                     AEPAVVAPAEVAPPAAAAPPEPVASAPARPAPPRELCADTRFLNRAVCLQNECSKPGL
                     RQHPQCVQMREQQDALRRGAGDH"
     gene            complement(811..2220)
                     /gene="yegQ"
                     /locus_tag="PKN51_12205"
     CDS             complement(811..2220)
                     /gene="yegQ"
                     /locus_tag="PKN51_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HNT85903.1"
                     /translation="MKAPELLLPAGSLDKMRAAYDFGADAVYAGQPRYSLRARNNEFR
                     LAQLAQGIAEAHARGKKFFVTSNLIAHNDKLRTYLRDLQPVIDLRPDALIMADAGLIM
                     QVREKWPHIPIHLSVQANTTNWAAVKFWQSVGVARIILSRELSLAEVEQIRQECPDME
                     LEVFVHGALCIAYSGRCLLSGYFNRRDPNQGTCTNACRWNYTTQAADVDPNTGEALAR
                     GQQLTGFNFANEQQRADQDGGARAARHPAADKVWLIEEEGRPGQLMPIMEDEHGTYIM
                     NSKDLRAVELVEKLAKIGVDSLKIEGRTKSLYYVARTAQVYRRAIDDAVAGRPFNPQL
                     ITELEGLANRGYTSGFLERRPAQDYQNYETGHSVAQRSQFVGEVRSVRDGWAEVETKN
                     RFAVGDWIEIIHPQGNRTVQLQHMKNADGQPIDVAPGNPLRVWIPVDGPAEQALIARL
                     MAPPQPAPAAASAPQGVTI"
     gene            2358..2867
                     /locus_tag="PKN51_12210"
     CDS             2358..2867
                     /locus_tag="PKN51_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNT85904.1"
                     /translation="MIREVEGDILLSQAQVIAHGIATHDPFDSGLALALRERFPSMVK
                     DYRHATHAKAPDTGEIWAWRGVNEDGSTRGIVNLLTQGMQSQDKAARPVKARLEDVNR
                     ALRALARHLQDEGVKSVALPRLATGVGALDWADVKPLIQQHLGGLGIPVLVYEVYHKG
                     MKADEKLAA"
     gene            3017..3634
                     /locus_tag="PKN51_12215"
     CDS             3017..3634
                     /locus_tag="PKN51_12215"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HNT85905.1"
                     /translation="MNKKLAALLALTLLGTAHAQSGLKAGLWENRTVRMMVDGKDMTA
                     AMRDAAELRKQQIAKLPPAQRQQAEAALARQGGDPLSRRVCISAEMAARQQPMVPRPP
                     RAECEPPKFKRDGQRTTFELSCTQEGGKLSGKGEIVTTADLITTRLETRSSDGAGQAH
                     TLLAETQMKYLGSDCGGIKPLEAPAQPASAAAATSRSPRKPAPAK"
     gene            3821..>6387
                     /locus_tag="PKN51_12220"
     CDS             3821..>6387
                     /locus_tag="PKN51_12220"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNT85906.1"
                     /translation="MATRNTHAFASTRKRFQTASGRSGELYSLPALARRFPNVKRLPV
                     SLRLVLESVLRHCDGVKVTPEHVEQLANWRPRAPRTDEIPFTVARVILQDFTGVPLMA
                     DLAAMRSTAQRLGQDPKKVEPLVPVDLVVDHSVMIDFYGKHNALDLNMKLEFQRNRER
                     YEFMKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHKTKDGTYYPDTLVGTDSHTTM
                     INGIGVVGWGVGGIEAEAAMLGQPVYFLTPDVVGFELKGRLREGCTATDLVLTVTERL
                     RQHKVVGKFVEFFGEGTASLSVPDRATIGNMAPEYGATMGFFPVDERTLEYFQGTGRS
                     KAEIEAFEAWFRAQNLFGVPRAGEIDYSQLITLDLGQVTPSVAGPKRPQDRIELGQLS
                     ARFAELFSLPMSDNGFNRPAAELHRRLEVARDNDPDSDERAPELEPLPLGAARSVVEM
                     VANKPTLESALSEADVVEPVSANRYTLGNGDVLIAAITSCTNTSNPSVMLAAGLLAKK
                     AVQAGLTVRPHIKTSLAPGSRIVTEYLSQTGLLPYLEQLGFPVSAYGCTTCIGNAGDL
                     APELNEAITRNQLVCAAVLSGNRNFEARIHPNLKANFLMSPPLVVAYAIAGNVMVDLM
                     TQPVGRGKGGKDVYLGDIWPSSDEVNRLLKYAMKGKAFRDNYAKVRSQPGQLWERVQG
                     VGGEVYDWPTSTYIAEPPFFDRFTIDSIAASAGYERAVTPSDPHAVEGARIMALFGDS
                     ITTDHISPAGSIKESSPAGQWLQAHGVGKQDFNSYGSRRGNHEVMMRGTFANVRIKNL
                     MLPSLADGSREEGGVTLMQPSGQKMFIYDAAMAYQAAGVPTVIFAGEEYGTGSSRDWA
                     "
BASE COUNT         1014 a         2193 c         2232 g          948 t
ORIGIN      
        1 gcgccgaacc gatttcagtg tttcagtgga caaggtttag tggtcaccgg cgccccggcg
       61 cagggcgtcc tgctgctcgc gcatctgcac gcactgcggg tgctggcgca gaccgggctt
      121 gctgcactcg ttctgcaggc agacggcgcg gttcagaaag cgtgtgtcgg cgcacagctc
      181 gcgcggcggc gccgggcggg cgggcgcgct ggccaccggc tcgggtggcg cagctgcggc
      241 aggcggcgcc acctcggccg gggcgacgac ggccggctcg gcggccggcg gcgatggctt
      301 gctgcgccgc gcggcggtgg atggacgcgt cggcgccgcc ggggccgcgc tggaggtgct
      361 tgggatcggc gcgctggatg ccgccggcag gggcggcggt gcagcgctgg ccgcgctggc
      421 tggcgacgcc ggctcgaccg cggctggcga cgaggaagcg gcggctcccg caggcgtggc
      481 ggcggcctgc ggcggcgccg tgtccggacg tgtgtcgccg cgccacaggg cgccgcccag
      541 ggcgaccacg gccaccagcg ccaccaggcc ccacagccag gcgtggcgtt tgcgcggcgg
      601 cgcctcggcc gggcgcttgc ggcgccggcg cttgcgcgcc ggctgctcca gcggcagcgg
      661 ctgggtcggc tcgctcaggg gtaccagctg atgcgcgcac ttcaggcaga acttggcggc
      721 atcgcggttt tccgcgccgc attgggggca gaggcgggac atgcgagggc tgggcggtcc
      781 ggggcgcagc gattaacctg aaagtatgaa tcagatggta acaccttgcg gcgccgacgc
      841 tgccgcaggc gccggctgag gcggcgccat caggcgcgcg atcagcgcct gctcggccgg
      901 cccatcgact ggaatccaca cgcgcagcgg gttgccgggc gccacgtcga tgggttggcc
      961 gtcggcgttc ttcatgtgct gcaactgcac ggtgcggttg ccctgcgggt ggatgatttc
     1021 gatccagtcg ccgacggcga atcggttctt ggtttcgacc tcggcccagc catcgcgcac
     1081 cgaacggact tcgcccacga actggctgcg ctgggccacg gaatggccgg tttcgtagtt
     1141 ctggtagtcc tgcgccgggc ggcgctccag aaagccgctg gtgtagccgc ggttggccag
     1201 gccttccagc tcggtgatga gctgcgggtt gaaggggcgg ccagccaccg cgtcgtcgat
     1261 ggcgcggcgg tacacctggg cggtgcgcgc cacgtagtac aggctcttgg tgcggccctc
     1321 gatcttgagc gagtcgacgc cgattttcgc cagcttttcc accagctcga ccgcgcgcag
     1381 gtccttgctg ttcatgatgt aggtgccgtg ctcgtcttcc atgatgggca tcagctggcc
     1441 ggggcggcct tcttcctcga tcagccagac cttgtcggcg gccgggtggc gtgcggctct
     1501 ggcgccgccg tcctgatcgg cgcgctgctg ctcgttggcg aagttgaagc cggtcagttg
     1561 ctggccgcgc gccagcgcct cgccggtgtt gggatcgacg tcggcggcct gggtcgtgta
     1621 gttccagcgg caggcgttgg tgcaggtgcc ctggttgggg tcgcgccggt tgaagtagcc
     1681 cgagagcagg cagcggcccg agtaggcgat gcacagcgcg ccgtgcacga acacctccag
     1741 ctccatgtcg gggcactcct ggcggatctg ctcgacctcg gccagcgaca gctcgcgcga
     1801 caggatgatg cgcgccacgc ccaccgactg ccagaacttc accgccgccc agttggtggt
     1861 gttggcctgc accgacagat ggatcgggat gtgcggccac ttttcgcgca cctgcatgat
     1921 cagcccggcg tcggccatga tcagggcgtc tggccgcagg tcgatcaccg gctgcagatc
     1981 gcgcaggtag gtgcgcagct tgtcgttgtg cgcgatcagg ttgctggtga cgaagaattt
     2041 cttgccgcgc gcgtgcgcct cggcaatgcc ctgggccagc tgcgccaggc gaaattcgtt
     2101 gttgcgcgca cgcaggctgt agcgcggctg gccggcgtac acggcatcgg cgccaaagtc
     2161 gtaggcggcg cgcatcttgt ccaggctgcc ggcgggcagc agcagttcgg gggctttcat
     2221 gggggcctat tgtcggcgca gggggccggg caggggcgcc aaagggcatc ggcttgacgg
     2281 cagtcaagaa ccatcaaccc tgactgggct aaggtcaaag catcgagaga ttcgcaaccc
     2341 gtcccaagga gaaaaccatg atccgtgaag tcgaaggcga cattctgctg agccaggctc
     2401 aggtgattgc gcacggcatc gccacccacg acccgttcga ttccggcctg gcgctggctc
     2461 tgcgcgagcg cttcccgtcg atggtcaagg actatcgcca cgccacccat gccaaggcgc
     2521 ccgacaccgg cgagatctgg gcctggcgcg gcgtcaacga agacggctcc acgcgcggca
     2581 tcgtcaacct gctgacccag ggcatgcaaa gccaggacaa ggccgcgcgc ccggtcaagg
     2641 ccaggctgga agacgtcaat cgcgcgctgc gcgccctggc caggcacctg caggacgaag
     2701 gcgtcaagag cgtggcgctg ccgcgcctgg ccaccggtgt cggcgcgctc gactgggccg
     2761 acgtcaagcc gctgatccag cagcacctgg gcggcctggg cattccggtg ctggtgtacg
     2821 aggtctatca caagggcatg aaggccgacg aaaagctcgc cgcctgagac gagcccctca
     2881 ggcctgcgtt ctgacgctcc cgcccgggcc acgcgcggcg cccgcgtcgg cgcaccagcg
     2941 cccgcaccgg ctttggtgcg ggcgcttttt ttcgtaccat cggcgcccca cgcatccttt
     3001 ggaaggtcga tcgctcatga acaagaagct cgccgcgctg ctggcgctca ccctgctggg
     3061 caccgcccat gcgcaatcgg gcctgaaggc gggcctgtgg gaaaaccgca ccgtccgcat
     3121 gatggtggac ggcaaggaca tgacggcggc catgcgtgac gcggccgagc tgcgcaagca
     3181 gcagatcgcc aagctgccgc ccgcgcagcg ccagcaggcc gaggcggcgc tggcgcgcca
     3241 gggcggcgac ccgctgtcgc ggcgcgtgtg catcagcgcc gagatggcgg cgcgccagca
     3301 acccatggtg ccgcgcccgc cgcgcgccga gtgcgagccg ccgaagttca agcgcgacgg
     3361 ccagcgcacc accttcgaac tcagctgcac ccaggaaggc ggcaagctga gcggcaaggg
     3421 cgagatcgtg acgaccgccg atctgatcac cacccgcctc gaaacccgca gcagcgacgg
     3481 cgccggccag gcccacaccc tgctggccga aacgcagatg aagtacctgg gcagcgactg
     3541 cggcgggatc aagccgctcg aagcgccggc gcaaccggcc tcggccgccg ccgcgaccag
     3601 ccgttcgcca cgcaagccgg cgcccgcgaa gtagcacccg gccagggctg aaatgctact
     3661 gaaaacgtag ctggcggcgc ttgccagaca agcgcagatc gagtttcggc tttggaatca
     3721 gggttgtggc cccgggtgct ggccggcccc gcctgggcct gcgacaatga ccttgggcgg
     3781 cgcggcgccg ccatcgccca tcgacccagg agcacacgcc atggccacgc gcaacaccca
     3841 tgccttcgcc agcacccgca agcgctttca gaccgcgtcg ggcaggagcg gcgagctgta
     3901 ttcgctgccg gcgctggccc ggcgcttccc caacgtcaag cgcctgccgg tgtcgctgcg
     3961 gctggtgctg gagtcggtgc tgcgccactg cgacggcgtc aaggtgacgc ccgagcacgt
     4021 cgagcaactg gccaactggc ggccgcgggc gccgcgcacc gacgagatcc cgttcaccgt
     4081 ggcgcgcgtg atcctgcagg acttcaccgg cgtgcccctg atggccgacc tggcggccat
     4141 gcgctccacc gcgcagcgcc tgggccagga cccgaagaag gtcgagccgc tggtgccggt
     4201 ggacctggtg gtcgaccact cggtgatgat cgacttctac ggcaagcaca atgcgctcga
     4261 cctgaacatg aagctcgaat tccagcgcaa ccgcgagcgc tacgagttca tgaaatgggg
     4321 catgcaggcc ttcgacacct ttggcgtggt gcccccgggc tttggcatcg tgcaccaggt
     4381 caacctcgaa tacctggcgc gcggtgtgca caagaccaag gacggcacct actaccccga
     4441 caccctggtg ggcaccgaca gccacaccac catgatcaac ggcatcggcg tggtcggctg
     4501 gggcgtgggc ggcatcgagg ctgaagccgc catgctgggc cagccggtgt acttcctcac
     4561 gcccgacgtg gtgggcttcg agctcaaggg ccggctgcgc gaaggctgca ccgccaccga
     4621 cctggtgctg acggtgaccg agcgcctgcg ccagcacaag gtggtgggca aattcgtcga
     4681 attcttcggt gagggcacgg ccagcctgag cgtgcccgac cgcgccacca tcggcaacat
     4741 ggcgcccgag tacggcgcca ccatgggctt cttcccggtc gacgagcgca ccctcgaata
     4801 ctttcagggc actggccgca gcaaggccga gatcgaagcc ttcgaagcct ggttccgggc
     4861 gcagaacttg tttggcgtgc cgcgcgccgg cgagatcgac tactcgcaac tcatcacgct
     4921 cgacctgggc caggtcaccc ccagcgtggc cggccccaag cggccgcagg accgcatcga
     4981 gctgggccag ctgtcggcgc gttttgccga gctgttttcg ctgcccatga gcgacaacgg
     5041 cttcaaccgc ccggcggccg aactgcaccg gcgcctggag gtggcgcgcg acaacgatcc
     5101 cgacagcgac gaacgcgccc ccgagctgga gccgctgccg ctgggcgcgg cgcgctcggt
     5161 ggtcgagatg gtggccaaca agccgacgct ggagtcggcc ctgtccgagg ccgacgtggt
     5221 cgagccggtg tcggccaacc gctacacgct gggcaatggc gacgtgctga tcgccgccat
     5281 caccagctgc accaacacca gcaaccccag cgtcatgctg gccgccggcc tgctggccaa
     5341 gaaggcggtg caggccggcc tgacggtgcg gccgcacatc aagacctcgc tggcgccggg
     5401 atcgcgcatc gtcaccgaat acctcagcca gaccggcctg ctgccctacc tggagcagct
     5461 gggctttccg gtgtcggcct acggctgcac cacctgcatc ggcaacgccg gcgacctggc
     5521 gcccgagctg aacgaggcca tcacccgcaa ccagctggtg tgcgcggccg tgctgtcggg
     5581 caaccgcaac ttcgaggcgc gcatccaccc caacctgaag gccaacttcc tgatgagccc
     5641 gccgctggtg gtggcctacg ccatcgccgg caacgtgatg gtcgacctga tgacgcagcc
     5701 ggtgggccgg ggcaagggcg gcaaggacgt gtacctgggc gacatctggc ccagcagcga
     5761 cgaggtcaac cgcctgctga agtacgccat gaagggcaag gccttccgcg acaactacgc
     5821 caaggtgcgc agccagccgg gccagctgtg ggagcgcgtc cagggcgtcg gcggcgaggt
     5881 ctacgactgg ccgaccagca cctacatcgc cgagccgcca ttctttgatc gatttactat
     5941 cgattcgata gctgccagcg caggctacga gcgcgctgta accccatctg acccgcatgc
     6001 cgtggagggt gcgcggatca tggcgctgtt tggcgactcg atcaccaccg accacatctc
     6061 gccggccggc agcatcaagg aaagctcgcc cgccggccag tggctgcagg cgcacggcgt
     6121 gggcaagcag gacttcaaca gctacggctc gcgccgcggc aaccacgagg tgatgatgcg
     6181 cggcaccttc gccaatgtgc gcatcaagaa cctgatgctc ccgtcgctgg ccgacggcag
     6241 ccgcgaggag ggcggcgtca ccctgatgca gccctcgggc cagaagatgt tcatctacga
     6301 cgccgccatg gcctaccagg ccgccggcgt gcccaccgtg atcttcgccg gcgaagagta
     6361 cggcaccggc tccagccgcg actgggc
//
LOCUS       DAOQTZ010000134         6369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13453_length_6369_cov_15.119417, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000134 DAOQTZ010000000
VERSION     DAOQTZ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13453_length_6369_cov_15.119417"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PKN51_12225"
     CDS             <1..1249
                     /locus_tag="PKN51_12225"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNT85907.1"
                     /translation="LAMVDSAKGITNFHSPNDVIVDASMPAMIRNGGKMWDANGRLKD
                     VKAVMPESTFARIYQEMINFCKWHGAFDPKTMGTVPNVGLMAQQAEEYGSHDKTFEIP
                     EDGVANITDLNTGEVLMSQNVEAGDIWRMCQVKDAPIRDWVKLAVTRARQSGMPVVFW
                     LDPYRPHEAQLITKVKMYLHEHDTTGLDIQIMSQVRAMRYTLERVIRGLDTISATGNI
                     LRDYLTDLFPIMELGTSAKMLSIVPLMAGGGMYETGAGGSAPKHVQQLVEENHLRWDS
                     LGEFLALAVSLEDLGIKNNNARAKLLARTLDAATGKLLDNNKGPSPRTGQLDNRGSHF
                     YLAMYWAQALAAQTEDAELAAKFAALAKTLTDNEARIVQELTDIQGQAVDIGGYYMPD
                     TAKLAQVMRPSATFNQALAALVG"
     gene            complement(1543..1986)
                     /locus_tag="PKN51_12230"
     CDS             complement(1543..1986)
                     /locus_tag="PKN51_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNT85908.1"
                     /translation="MLALSCSAALAQRAPTGQPQLDLPRTTLTAGMHRIEVQLASTPV
                     QRQIGLMHRQSMPAHEGMLFTFDEPGVQCFWMKNTLIPLTAAFVADDGTIVNLADMKP
                     LDESSHCSARPVRYVLEMNQGWFAQRHLKAGHRLGGGPFSKPAAR"
     gene            2496..3749
                     /gene="icd"
                     /locus_tag="PKN51_12235"
     CDS             2496..3749
                     /gene="icd"
                     /locus_tag="PKN51_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNT85909.1"
                     /translation="MYQHIQVPAGGQKITVNADGSLNVPDQPIIPFIEGDGTGVDITP
                     VMIKVVDAAVARCYGGRRRIHWMEVYAGEKSTRVYGPDVWLPEETLAALKEYVVSIKG
                     PLTTPVGGGIRSLNVALRQELDLYVCLRPVRYFKGVPSPVKEPEKVDMVIFRENSEDI
                     YAGIEWEARSAKAQQLIKFLVEDMGVAKIRFPATSGIGVKPVSIEGTERLVRKAIQYA
                     IDNDRPSVTLVHKGNIMKHTEGGFRDWGYALAQREFGAELLDGGPWCQFRNPKTGRNI
                     VVKDAIADAFLQQILLRPAEYSVIATLNLNGDYISDALAAQVGGIGIAPGANLSDSVA
                     CFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWTEAADLIITSMEAAIQSKRVTY
                     DFARLMEGAVQVSCSGFGQVMIDHM"
     gene            complement(3819..4091)
                     /locus_tag="PKN51_12240"
     CDS             complement(3819..4091)
                     /locus_tag="PKN51_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNT85910.1"
                     /translation="MPEGKVKWFNDLKGFGFIEPDGGGPDVFAHFSAIDMDGFKTLKE
                     GARVSYEVNQGPKGLLATRIRTDPMAAVPTPLPNAGALPDQAPLPH"
     gene            4222..4599
                     /gene="clpS"
                     /locus_tag="PKN51_12245"
     CDS             4222..4599
                     /gene="clpS"
                     /locus_tag="PKN51_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNT85911.1"
                     /translation="MIFMASTPPTRSPALPGPVTPVRQPEGGGEVVLERVPQKVKPPQ
                     MYQVVMLNDDYTPMEFVVVVIQEFFGKDRESATQIMLKIHLDGRGVCGVYSRDVAATK
                     VEQVLDAARQAGHPLQCVSEPVE"
     gene            4740..>6369
                     /locus_tag="PKN51_12250"
     CDS             4740..>6369
                     /locus_tag="PKN51_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT85912.1"
                     /translation="MIAQELEVSLHMAFVEARQQRHEFITVEHLLLALLDNPSAAEVL
                     RACAANIDDLRKSLTNFIKDNTPQVAGSDEVDTQPTLGFQRVIQRAIMHVQSTGNGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNFIAHGIRKTDTPEASKPAEGQPS
                     PGEAEEASEKSEKASPLEQFTQNLNQLARDGKIDPLIGRDYEVERTIQILCRRRKNNP
                     LLVGEAGVGKTAIAEGLAWRITEGTVPEILEGATVYALDMGALLAGTKYRGDFEQRLK
                     GVLKSLKDRPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGQLKCIGATTFTE
                     YRGIFEKDAALSRRFQKVDVVEPSVAETIEILKGLKSRFEEHHAVKYATAALQAAAEL
                     SAKYITDRHLPDKAIDVIDEAGAAQRIAAASKRKKTIGKHEIEEIVAKIARIPPASVS
                     NDDRSKLQTLERDLKSVVFGQDQALDVLAAAVKMARSGLGKGDKPIGSFLFSGPTGVG
                     KTEAARQLAFIMGVELIRFDMSEYMERHAVSRLIG"
BASE COUNT         1185 a         2117 c         2125 g          942 t
ORIGIN      
        1 gctcgccatg gtcgattcgg ccaagggcat caccaacttc cactctccca acgacgtgat
       61 cgtcgacgcc tcgatgccgg ccatgatccg caacggcggc aagatgtggg atgccaacgg
      121 gcgcctcaag gacgtcaagg ccgtgatgcc cgaatcgacc ttcgcccgca tctaccagga
      181 gatgatcaac ttctgcaagt ggcatggcgc cttcgacccc aagaccatgg gcacggtgcc
      241 caacgtcggc ctgatggcgc agcaggccga ggaatacggc agccacgaca agaccttcga
      301 gatccccgag gacggcgtgg ccaacatcac cgacctgaac accggcgagg tgctgatgag
      361 ccagaacgtc gaggccggcg acatctggcg catgtgccag gtcaaggacg cgccgatccg
      421 cgactgggtc aagctcgccg tcacgcgcgc gcgccagtcg ggcatgccgg tggtgttctg
      481 gcttgacccg taccgtccgc acgaggcgca gctcatcacc aaggtcaaga tgtacctgca
      541 cgagcacgac accaccggcc tggacatcca gatcatgagc caggtgcgcg ccatgcgcta
      601 cacgctcgag cgcgtgatcc gcggcctgga caccatcagc gccaccggca acatcctgcg
      661 cgactacctg accgacctgt tccccatcat ggagctgggc accagcgcca agatgctgtc
      721 catcgtgccg ctgatggccg gcggcggcat gtacgaaacc ggcgccggcg gctcggcgcc
      781 caagcacgtg cagcagctgg tggaggaaaa ccacctgcgc tgggacagcc tgggcgagtt
      841 cctggccctg gccgtgagcc tggaagatct gggcatcaaa aacaacaacg cccgcgccaa
      901 gctgctggcc aggacactgg acgccgccac cggcaagctg ctggacaaca acaagggccc
      961 cagcccgcgc accggtcagc tggacaaccg cggcagccac ttctacctgg ccatgtactg
     1021 ggcgcaggcg ctggcggcgc agaccgagga cgccgagctg gcggccaagt tcgcggcgct
     1081 ggccaagacg ctgaccgaca acgaggccag gatcgtgcag gaactcaccg acatccaggg
     1141 ccaggcggtc gacatcggcg gctactacat gcccgacacg gccaagctgg cgcaggtgat
     1201 gcgccccagc gccaccttca accaggcgct ggcggcgctg gtcggctgag accaccaggt
     1261 gcgctgcgcc gccgtggcgg cggcgcagcg gacccgggcg cggtggactc accgcgcccc
     1321 aattttttgc gcggcagcgg ccgcaggcgg cgcccagggt gttcgttggg gtattggcct
     1381 tttctggcct cgggtcaggc gcagcatgcg ccgcaagcta tcgaaatgat agctgtggca
     1441 acgctgaacc agcttttcgg gcgcgtcgcc ttcctgcctt gggcggcttg cgccttgctc
     1501 atgcagggcg cgccgcggtg ccggaaccga tgcggcgtgg cctcagcgcg ccgccggttt
     1561 gctgaacggc ccgccgccca ggcggtgtcc ggccttcagg tggcgttggg cgaaccagcc
     1621 ttggttcatc tccagcacgt aacgcaccgg cctggccgag caatggctgc tttcatccag
     1681 cggcttcatg tcggccaggt tgacgatggt gccgtcgtcg gccacgaagg cggcggtgag
     1741 agggatcagc gtgttcttca tccagaagca ctgcacgccc ggctcgtcga aggtgaacag
     1801 catgccctcg tgcgccggca tgctctggcg gtgcatcagg ccgatctggc gctgcaccgg
     1861 cgtgctggcc agctgcacct cgatgcggtg catgccggcc gtgagcgtgg tgcgcggcag
     1921 gtccagttgc ggttgaccgg tcggcgcgcg ctgggcgagc gcggccgagc aggacagggc
     1981 cagcatgagc aggtgcaggg ggcggcaggc gagaacgggc atggcgacag cggggatggg
     2041 aacaaggggt cgggcgtcgg cggcgacccg aaggtggggc ccgcgccggc gtccagcgcg
     2101 cattgtcgcc gccacgcgct ttgccctggc gtgcgccgca aggcggtggt tggtgtggtg
     2161 cgccgctatg gcttgcactt tcagcgcgtc gctgagcggc tcgatgcaag tcgcgcacgc
     2221 gcagcaatgc gtgcgccttt caagcgggcg caacgccgca ggcagtcgct cagcggctcg
     2281 cccaaaggga gcccacggaa ggcgcccatg ccgcgttgca gcccttgcca agggatctgc
     2341 catgggctgc gggctgcgcc ttgcctggac gccttctggg gtctctgaaa gcacaagcca
     2401 tagcggcgca ccacactaga atcgattccg ccccgagcgc catgccagcc ggcgtgcgcg
     2461 cccaggctgg cccatcaacc ttggagaacc catccatgta ccagcacatc caggtgcctg
     2521 ccggcggaca gaagatcacc gtcaacgccg atggctcgct caacgttccg gaccagccca
     2581 tcatcccctt catcgaaggt gacggcacgg gcgtggacat cacgccggtg atgatcaagg
     2641 tggtggatgc cgcggtggcc aggtgctatg gcgggcggcg caggatccac tggatggagg
     2701 tgtatgcggg cgagaaatcc acccgtgtct acggccccga cgtctggctg cccgaagaga
     2761 cgctggcggc gctcaaggag tacgtggtct ccatcaaggg gccgctgacc accccggtcg
     2821 gcggcggcat ccgctcgctc aacgtggcgc tgcgccagga gctcgatctg tacgtctgcc
     2881 tgcgcccggt gcgctacttc aagggcgtgc cctcgccggt gaaggagccg gagaaggtcg
     2941 acatggtgat ctttcgcgag aacagcgagg acatctacgc cggcatcgaa tgggaggcca
     3001 ggagcgccaa ggcgcagcag ctcatcaagt tcctggtcga ggacatgggt gtggccaaga
     3061 tccgctttcc ggccacctcc ggcatcggcg tcaagccggt gtcgatcgaa ggcaccgaac
     3121 gcctggtgcg caaggccatc cagtacgcca tcgacaacga caggcccagc gtcaccctgg
     3181 tgcacaaggg caacatcatg aagcacaccg aagggggctt ccgtgactgg ggttacgcgc
     3241 tggcgcagcg cgaattcggg gccgaactgc tggacggcgg gccgtggtgc cagttcagga
     3301 acccgaagac gggccgcaac atcgtcgtca aggacgccat cgccgatgcc ttcctgcagc
     3361 agatcctgct gcgtccggcc gagtattcgg tgatcgccac gctgaacctt aacggcgact
     3421 acatcagcga cgcgctggcg gcgcaggtgg gtggcatcgg catcgcgccg ggcgccaacc
     3481 tgagcgacag cgtggcctgc ttcgaagcca cccacggcac ggcgcccaag tacgccggca
     3541 aggactacgt caaccccggc tccgaaatcc tcagcgccga aatgatgctg cgccacatgg
     3601 gctggaccga ggcggccgac ctgatcatca ccagcatgga agcggccatc cagagcaagc
     3661 gcgtcaccta cgacttcgcc cgcctgatgg aaggcgcggt gcaggtcagc tgctcaggct
     3721 tcggtcaggt gatgatcgat cacatgtgag cgccgccggc ccaaaaaaaa tcccgagccg
     3781 ggctcgggat cggggtgagg aggtgaccgg ctggccggtc aatgcggcag gggcgcctgg
     3841 tcgggcagcg cgccggcgtt cggcaggggc gtgggcaccg ccgccatcgg atcggtgcga
     3901 atccgcgtcg ccagcaggcc cttggggccc tggttgactt catagctgac gcgcgcgcct
     3961 tccttcaggg tcttgaaccc gtccatgtcg atggccgaga aatgtgcgaa cacgtcgggc
     4021 ccgccgccgt ccggctcgat gaagccgaaa cccttcaggt cattgaacca cttgactttt
     4081 ccttcgggca tgtcttgtct cctgatgtct tccgtccgcc ggcaagtgtg cgggaagggt
     4141 tttgcccttg tcaacccgag gcgccgtccg ggcgggcgcg gaaagccctg cgcccgtcgg
     4201 cgggcaggcg cgctcattag aatcattttc atggcgagca ctcctccgac acgttccccg
     4261 gccctgccgg ggcccgtcac ccccgttcgg caacccgaag gtggcggcga ggtggtgctc
     4321 gagcgggtgc cgcagaaggt caagccgccg cagatgtacc aggtggtgat gctcaatgac
     4381 gactacacgc cgatggaatt cgtggtcgtg gtgatccagg aattcttcgg caaggaccgc
     4441 gagagcgcca cccagatcat gctcaagatc cacctcgacg gccgcggcgt gtgcggcgtc
     4501 tattcgcggg atgtggcggc gaccaaggtc gagcaggtgc tggacgccgc gcgccaggcg
     4561 gggcacccgc tgcagtgcgt cagcgagccg gtggaatagg cacctggggc cggcgccgcc
     4621 tggaagacga accgaagcag gagccggacc atgccgttga atatcaccga acttccccca
     4681 actgtcgctg tcaaaacctc gggaaagtcg taagcttgag gtgctagagg aaccacacca
     4741 tgatcgccca ggaactggaa gtcagtctgc acatggcctt tgtcgaggcc cggcagcagc
     4801 gccatgagtt catcaccgtc gagcacctgc tgctggccct gctggacaac ccgagcgccg
     4861 ccgaggtgct gcgcgcctgc gcggccaaca tcgatgacct gcgcaagtcg ctgaccaact
     4921 tcatcaagga caacacgccg caggtcgccg gcagcgacga ggtggacacg cagccgacgc
     4981 tgggcttcca gcgcgtgatc cagcgcgcca tcatgcacgt gcagtccacc ggcaacggca
     5041 agaaggaagt gaccggcgcc aacgtgctgg tggcgatttt cggcgagaag gattcgcacg
     5101 ccgtctacta cctgcaccag cagggcgtca cccgcctgga cgtggtcaat ttcatcgccc
     5161 acggcatccg caagaccgac acgccggagg ccagcaagcc tgccgaaggc cagcccagcc
     5221 ccggcgaggc cgaagaggcg agcgaaaaga gcgagaaggc ctcgccgctg gagcaattca
     5281 cgcagaacct gaatcagctc gccagggacg gcaagatcga tccgctgatc gggcgcgact
     5341 acgaggtcga gcgcaccatc cagatcctgt gccggcggcg caagaacaac ccgctgctgg
     5401 tgggcgaggc gggtgtcggc aagaccgcca tcgccgaagg cctggcctgg cgcatcaccg
     5461 aaggcacggt gcccgagatc ctggaaggcg ccaccgtcta cgcgctcgac atgggcgcgc
     5521 tgctggccgg caccaagtac cgcggcgact tcgagcagcg tctcaagggc gtgctcaaga
     5581 gcctcaagga ccggcccaac gccatcctgt tcatcgacga gatccacacc ctgatcggtg
     5641 ccggcgcggc ctccggcggc acactggatg ccagcaacct gctcaagccg gcactgtcga
     5701 gcggacagct caagtgcatt ggcgccacca ccttcaccga gtaccgtggc atcttcgaga
     5761 aggacgcggc gctgtcgcgg cgcttccaga aggtcgatgt ggtggagccg agcgtggctg
     5821 aaaccatcga aatcctcaag ggcctcaaga gccgcttcga ggagcaccat gccgtcaagt
     5881 acgccaccgc ggcgctgcag gctgcggccg agctgtcggc caagtacatc accgaccgcc
     5941 atttgcccga caaggccatc gacgtgatcg acgaagccgg cgcggcccag cgcatcgcgg
     6001 cggccagcaa acgcaagaaa accatcggca agcacgagat cgaagagatc gtcgccaaga
     6061 tcgcacgcat cccgccggcc agcgtcagca acgacgaccg cagcaagctg cagacgctgg
     6121 agcgcgacct caagagcgtg gtgttcggcc aggaccaggc gctcgacgtg ctggcggctg
     6181 ccgtcaagat ggcgcgctcc ggcctgggca agggcgacaa gccgatcggc agcttcctgt
     6241 tctccggccc caccggcgtc ggcaaaaccg aggcggcgcg ccagctggcc ttcatcatgg
     6301 gggtcgaact gatccgcttc gacatgtccg agtacatgga gcgccacgcc gtcagccgcc
     6361 tgatcggcg
//
LOCUS       DAOQTZ010000135         6321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13595_length_6321_cov_13.234599, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000135 DAOQTZ010000000
VERSION     DAOQTZ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13595_length_6321_cov_13.234599"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..962
                     /locus_tag="PKN51_12255"
     CDS             126..962
                     /locus_tag="PKN51_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT85913.1"
                     /translation="MNPLASPSRRLLLASAAATALLWGCSKTETPAPAAPEPAAPAAA
                     TAPGLPARIVIGLDDNFPPMGFRDDKNQLVGFDIDLAREAGKRLGIEVEFKPIDWNAK
                     EAELSGKRVDVLWNGLTITEERKKNIAFTAPYMQNHQIVVVKADAAIKAKADLAGKVV
                     GVQDGSSAVEAIEKDAATAQSFKQLKKYGDNVTALMDLSAGRVDAVVLDEVVGRYYSA
                     KKPGEYAVLDEHFGTEDYGVGLRQDDAALRTRLDEVLTAMKGDGSGARIAEQWFGKNI
                     LK"
     gene            1136..1789
                     /locus_tag="PKN51_12260"
     CDS             1136..1789
                     /locus_tag="PKN51_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNT85914.1"
                     /translation="MDYLLRILPPLIEGAGVTLGLFVVTLALSLPLGLALALARLSRW
                     GALRRAVSGFVWLMRGTPLMLQLLFVYYALPFVPVIGVRLPDYPAAVVAFTLNYAAYF
                     AEILRAGIQSIDRGQYEGAKALGMSYAQTMRRIVMPQVWRGSLPPLANETITLVKDTS
                     LIYVLALNDLLRAARGIVQRDFSITPFVVAAAFYLLMTLVLTWIFQRLERRHATHVA"
     gene            1779..2555
                     /locus_tag="PKN51_12265"
     CDS             1779..2555
                     /locus_tag="PKN51_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNT85915.1"
                     /translation="MSPEQRPPMVRASGLHKHFGALHVLRGVSLTVERGEVVAILGPS
                     GSGKSTLLRCLNHLETIERGCIEIAGETLASADAAGASSYPSDSEVRRICQRTGMVFQ
                     HFNLFPHLSVLGNLIEAPITVRKLARAEVVPRAEALLAKVGLADKRDVYPGRLSGGQK
                     QRVAIARALAMQPDILLFDEPTSALDPELTGEVLRTMRQLAEERMTMLVVTHEMAFAR
                     EVAHRVVFMDAGQIVEDRPAADFFAAPRHERARAFLAHML"
     gene            complement(2583..6206)
                     /locus_tag="PKN51_12270"
     CDS             complement(2583..6206)
                     /locus_tag="PKN51_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HNT85916.1"
                     /translation="MYPVEQRWRPFEESREHDLNDAIAAAVDELWVGPPQGDILVFLP
                     GEREIREAADHLRAHLSHRPLTRGAEVLPLFARLSQAEQDRVFEPHGAPRIVLATNVA
                     ETSLTVPGIRYVIDPGTARVKRYSYRSKVEQLLVEPISQAAAGQRAGRCGRVADGICI
                     RLYDEQDFAGRPRFTDPEILRSSLAAVILRMKSLRLGGEGGRVEDFPFLDMPSGRAIA
                     DGVQLLQELGALDEAQQLTALGTELARLPLDPRVGRMILEARARGALREVLVIAAALS
                     VPDVRDRPLEQQAQADQAHAKFDDEKSEFSGYLRLWKWLHDARGGTGPHASRFAAALP
                     PRGAAAGLGRPGAGRSLPMTRAQMAASEGQTAARKPANAAALPVAQRAAAALAQARQT
                     LSKKELPALSGQGLDSDLIQNAEASAVHKLSNRQWEQLLRQNFISVRRVREWRDIHTQ
                     LHTVVAEHKWRENDQPSGYEALHKSLLAGLLGNVGCKLEGDDAQSGEYLGARGIKFHR
                     HPGAHLSKRPGKWIVCAELVETTRLFGRGIAAIEPQWLEEVGGHLLKKQLLEPHWEKK
                     AQDVVALERATLYGLLVYGGRRRSFGAVDPRAAREIFIRAALVGGDWPEEWARRLPFL
                     LANQRTIAKVEELEHKSRRQDVLVDEELIYAFYDSQVPADICNGRDFERWWREASRAN
                     PNLLLLTREELMRHEAAGITTSAFPKMVRLGGVDCAASYLHQPGDARDGLTVTVPLFV
                     LNQVSEARCEWLVPGMLKDKIQALLKSLPQKPRSRFVPLPDSAARLAEILGAPEVFGH
                     GSLTDALLRRVREATSLDVKRSDFKLDMLPAHLFMNFRVVDEHGRQRGMGRHLAALKA
                     ELGGEARGAFQALAALKGAVPGAAPAAAPGPQDSEQNQAPAQAGKAPAAIKNVVTEIA
                     STDIRYTGWDFGVLPELMEIRQRGQVLVGFPALIDAGDAVTIEVFDEPELAAARHRQG
                     LRRLCALQIRDALKYLEKNVPDLQKMAVAYMQVGRTADGAGGGTAEELRQQIIDVALE
                     RAFLVEPLPASADAFRQRVDEGRARLTLITGEVARLAGAILAEHAVALRKIKDTKGAP
                     EATRDAAEQLQRLMPRNFIAATPWPALQHFVRYLKAITLRLDKLRADPARDAQRMAEV
                     RAQDQRFGRLVAARKGQQDARMQELRWLLEELRVSLFAQELRTPQPVSVKRLEKVWAQ
                     MAG"
BASE COUNT          911 a         2288 c         2145 g          977 t
ORIGIN      
        1 gcagctggcc ccttggcgac tcgctataag tacctgacag cgcgaaacac tgcactaaca
       61 ttacgggttt tcccgtagcg cgctgcggcg cgccccgttc gattccccat cccccgagcc
      121 tgagcatgaa cccgcttgcc tccccttccc gccgcctgct gctggccagc gccgccgcca
      181 ccgccctgct gtggggctgc agcaagaccg aaaccccggc cccggccgca cccgagcctg
      241 ccgcgcccgc ggccgccact gcgcctgggc tgccggccag aatcgtcatc ggcctggacg
      301 acaacttccc gcccatgggt tttcgcgacg acaagaacca gctggtcggc ttcgacatcg
      361 acctggcgcg cgaggccggc aagcgcctgg gcatcgaagt cgagttcaag ccgatcgact
      421 ggaacgccaa ggaggccgaa ctcagcggca agcgcgtcga cgtgctgtgg aacggcctga
      481 ccatcaccga agaacgcaag aaaaacatcg ccttcaccgc gccctacatg cagaaccacc
      541 agatcgtcgt ggtcaaggcc gacgcggcga tcaaggccaa ggccgatctg gccggcaagg
      601 tggtgggcgt gcaggacggc agcagcgccg tcgaggccat cgagaaggac gccgccaccg
      661 cccagagctt caaacagctc aagaagtacg gcgacaacgt gacggcgctg atggacctgt
      721 cggccggccg cgtcgacgcc gtggtgctgg acgaggtggt gggccgctac tacagcgcca
      781 agaagccggg tgagtatgcg gtgctggacg agcacttcgg caccgaggac tacggcgtcg
      841 gcctgcgcca ggacgatgcc gccctgcgca cgcggctgga cgaggtactg acggccatga
      901 agggcgacgg cagcggcgcc aggattgccg agcagtggtt tggcaagaac atcttgaagt
      961 gacccacccc cgtggctcca cgcgctgcgc gatgttgcgc ctcccccctc aagggggcgc
     1021 agcctgtggc caggcaaacc ccggccacgg ctgccgcggg catggcctgc tccgcagcca
     1081 cgcgactcgt ttcgggtcaa ccacctgacc cccaaaaacc gcaccagcat cggtgatgga
     1141 ctacctgctg cgcatcctgc caccgctgat cgaaggcgcc ggcgtcacgc tgggcctgtt
     1201 cgtggtcacg ctggcgctgt cgctgccgct cggcctggcg ctggcgctgg cgcggctgtc
     1261 gcgctggggc gcgctgcgcc gcgccgtcag cggcttcgtc tggctgatgc gcggcacgcc
     1321 gctgatgctg cagctgctgt tcgtctacta cgccctgccc ttcgtgccgg tgatcggcgt
     1381 gcgcctgccc gactacccgg cggcggtggt cgccttcaca ctcaactacg cggcctactt
     1441 tgccgaaatc ctgcgcgccg gcatccagtc gatcgaccgc ggccagtacg aaggcgccaa
     1501 ggcgctgggc atgagctacg cccagaccat gcgccgcatc gtcatgcccc aggtgtggcg
     1561 tggcagcctg ccgccgctgg ccaacgagac catcaccctg gtcaaggaca cctcgctgat
     1621 ctacgtgctg gcgctcaacg acctgctgcg cgccgcgcgc ggcatcgtgc agcgcgactt
     1681 cagcatcacg cccttcgtgg tcgccgccgc cttctacctg ctgatgacgc tggtactgac
     1741 ctggatcttt cagcgcctgg agcgccgcca cgccacccat gtcgcctgaa caacgccccc
     1801 ccatggtgcg cgccagcggc ctgcacaagc actttggcgc gctgcacgtg ctgcgcggcg
     1861 tgtcgctgac ggtcgagcgc ggcgaggtgg tggccatcct ggggccgtcg ggctcgggca
     1921 aatcgaccct gctgcgctgc ttgaaccacc tcgaaacgat cgagcgcggt tgcatcgaaa
     1981 tcgcaggcga aacgctcgcc agcgccgatg cggcgggcgc cagcagctat ccttctgata
     2041 gcgaagtgcg ccgcatctgc cagcgcaccg gcatggtgtt ccagcatttc aacctgttcc
     2101 cgcacctgag cgtgctgggc aacctgatcg aggcgccgat cacggtgcgc aagctggcgc
     2161 gcgccgaggt cgtgccgcgc gccgaggcgc tgctggccaa ggtgggtctg gccgacaagc
     2221 gcgacgtcta ccccggccgg ttgtcgggcg gccagaagca gcgcgtggcg atcgcgcggg
     2281 cgctggccat gcagcccgac atcctgctgt tcgacgagcc gaccagcgcg ctcgaccccg
     2341 aactcaccgg cgaggtgctg cgcaccatgc gccagctggc cgaagagcgc atgaccatgc
     2401 tggtggtcac gcacgagatg gcgtttgcgc gcgaggtggc gcaccgcgtg gtgttcatgg
     2461 acgccggcca gatcgtcgaa gaccggccag cggccgactt cttcgccgcg ccccgacacg
     2521 agcgcgcccg cgccttcctg gcccacatgc tgtgagccgg cggcgtcctg aggtggcccc
     2581 gttcaacctg ccatctgcgc ccacaccttt tccagccgct tgacgctgac cggctgcggc
     2641 gtgcgcagct cctgcgcgaa caggctcacg cgcagctcct cgagcagcca acgcaactcc
     2701 tgcatgcgcg catcctgctg gcccttgcgg gcggccacca ggcgcccgaa gcgctggtcc
     2761 tgcgcccgca cctcggccat gcgctgggcg tcgcgcgccg ggtcggcgcg cagcttgtcc
     2821 aggcgcaggg tgatggcctt caggtagcgc acgaagtgct gcaacgccgg ccagggggtg
     2881 gcggcgatga agttcctggg catcagccgc tgcagctgct cggccgcgtc gcgcgtcgcc
     2941 tcgggcgcgc ccttggtgtc cttgatcttg cgcagcgcca cggcgtgctc ggccaggatg
     3001 gcgccagcca gtcgcgccac ttcgccggtg atcagggtca ggcgcgcgcg gccttcgtcg
     3061 acgcgctggc ggaacgcatc ggcactggcg ggcagcggct cgaccagaaa ggcgcgctcc
     3121 agcgccacgt cgatgatctg ctggcgcagc tcctcggcgg tgccgccacc ggcgccgtcg
     3181 gccgtgcggc cgacctgcat gtaggcgacc gccatcttct gcagatcggg gacgttcttt
     3241 tccaggtact tcagggcgtc gcggatctgc aatgcgcaca ggcggcgcag gccctggcgg
     3301 tggcgcgcgg cggccagctc gggctcgtcg aacacctcga tggtgacggc gtcgccggcg
     3361 tcgatgagcg ccggaaaccc caccagcacc tgaccgcgct gacggatctc catcagctcg
     3421 ggcagcacgc cgaagtccca gccggtgtag cggatgtcgg tggaggcgat ctcggttact
     3481 acgttcttga tagctgctgg cgctttccca gcctgcgctg gagcctgatt ttgctctgaa
     3541 tcctgcgggc ctggcgcggc cgccggcgcg gccccgggga cggcgccctt gagcgccgcc
     3601 agggcctgaa aggcgccgcg cgcctcgccg cccagctcgg ccttcagcgc cgccagatgg
     3661 cgtcccatgc cgcgctggcg gccgtgctcg tcgaccacgc ggaagttcat gaacaggtgc
     3721 gccggcagca tgtcgagctt gaagtcgctg cgcttgacat ccaggctggt ggcctcgcgc
     3781 acgcgcctga gcagcgcgtc ggtcaggctg ccgtggccga acacctcggg cgcgcccagg
     3841 atctcggcca gccgggcggc gctgtcgggc agcggcacga agcggctgcg cggcttttgc
     3901 ggcaggcttt tcagcagcgc ctggatcttg tccttcagca tgccgggcac cagccattcg
     3961 cagcgcgcct cgctcacctg gttgagcacg aacagcggca ccgtcaccgt caggccgtcc
     4021 ctggcgtcgc ccggttggtg caggtagctg gcggcgcagt ccacgccgcc caggcgcacc
     4081 atcttgggaa aagcgctggt ggtgatgccg gcggcctcgt ggcgcatcag ctcctcgcgc
     4141 gtgagaagca gcaggttggg gttggcgcgg ctggcttcgc gccaccagcg ctcgaagtcg
     4201 cgcccgttgc agatgtcggc cgggacctgg ctgtcgtaga aggcgtagat cagctcctcg
     4261 tcgaccagca cgtcctggcg gcggctcttg tgctccagct cctcgacctt ggcgatggtg
     4321 cgctggttgg ccagcagaaa gggcaggcgc cgcgcccact cctcgggcca gtcgccgccc
     4381 accagcgccg cgcggatgaa gatctcgcgc gcggcgcgcg gatcgaccgc accgaagctg
     4441 cggcgccggc cgccgtagac cagcaggcca tagagcgtgg cgcgctccag cgccaccacg
     4501 tcctgcgcct tcttttccca gtgcggctcc agcagctgct ttttcagcag atggccgccg
     4561 acctcttcca gccactgcgg ctcgatggcg gcgatgccgc ggccgaacag gcgggtggtt
     4621 tccaccagct cggcgcagac gatccacttg cccggccgct tgctcaggtg cgcgcccgga
     4681 tggcggtgga acttgatgcc gcgcgcgccc agatactcgc cgctctgggc gtcgtcgccc
     4741 tccagcttgc agccgacgtt gcccagcagc cccgccagca gcgatttgtg cagcgcctcg
     4801 tagcccgaag gttggtcgtt ttcgcgccac ttgtgctcgg ccaccacggt gtgcaactgg
     4861 gtatgaatgt cgcgccactc gcgcacccgg cgcacgctga tgaagttctg gcgcagcagt
     4921 tgctcccact ggcggttgct cagcttgtgc accgccgacg cttcggcgtt ctgaatcaaa
     4981 tcggaatcaa gcccttgtcc agaaagcgcc ggaagctcct tttttgatag tgtctggcgt
     5041 gcttgggcaa gggccgctgc cgcgcgctgg gccactggca gcgcggccgc attggcgggt
     5101 ttgcgcgcgg ccgtctgccc ttcgctggct gccatctgcg cgcgggtcat aggcagtgac
     5161 cggccggcgc cgggccgccc caagccggcc gcggcccccc tggggggcag cgcagcagcg
     5221 aagcgcgaag cgtgggggcc tgttccaccg cgcgcgtcgt gcagccactt ccacagccgc
     5281 aggtagccgg aaaactcgct cttctcgtcg tcgaacttgg cgtgcgcctg atcggcctgg
     5341 gcctgctgct ccagcggccg gtcgcgcacg tcgggcacgc tcagcgcggc ggcgatcacc
     5401 agcacctcgc gcagcgcgcc gcgcgcgcgt gcctccagaa tcatgcggcc gacgcgcggg
     5461 tccagtggca ggcgcgccag ctccgtgccc agcgcggtga gctgctgcgc ctcgtccagc
     5521 gcgcccagct cctgcagcag ttgcacgccg tcggcgatgg cccggcccga aggcatgtcg
     5581 aggaagggaa agtcctccac ccgcccgccc tcgccgccca ggcgcaggct cttcatgcgc
     5641 aggatcacgg ccgccagcga ggagcgcagg atctccgggt cggtgaaacg cgggcggccg
     5701 gcgaagtcct gctcgtcgta caggcggatg cagatgccgt cggccacgcg cccgcagcgg
     5761 ccggcgcgct ggcccgccgc cgcctggctg atcggctcga ccagcaactg ctccaccttg
     5821 ctgcgataac tgtagcgctt gacgcgcgcc gtgccggggt cgatcacgta gcggataccc
     5881 ggcaccgtga gcgaggtctc ggccacgttg gtggccagca cgatgcgcgg cgcgccgtgc
     5941 ggctcgaaca cgcggtcctg ctcggcctgc gacagccgcg cgaacagcgg cagcacctcg
     6001 gcgccgcgcg tcagcggccg gtgcgacagg tgcgcgcgca ggtggtcggc ggcttcgcgg
     6061 atctcgcgct cgccgggcag aaacaccagg atgtcccctt gcggagggcc cacccaaagc
     6121 tcatccaccg cagcggcgat ggcgtcgttc aggtcgtgtt cgcggctttc ctcgaacggg
     6181 cgccagcgct gctccaccgg gtacatgcgc cccgacacct gcagcaccgg cgcagggaca
     6241 aggcccccac gctcccccac ttcgtgtggg tcgctgcccc ccgagggggc cacgcccggc
     6301 ttggggcggc ccggcgccgg g
//
LOCUS       DAOQTZ010000136         6259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13789_length_6259_cov_14.219213, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000136 DAOQTZ010000000
VERSION     DAOQTZ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13789_length_6259_cov_14.219213"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /gene="mraW"
                     /locus_tag="PKN51_12275"
     CDS             <1..382
                     /gene="mraW"
                     /locus_tag="PKN51_12275"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNT85917.1"
                     /translation="AVKTREPGQNPATRTFQAVRIFVNAELEELEQALEAAVQVLQPG
                     GRLVVISFHSLEDRIVKQFIARHARAPVDRRAPFAEPAPPRLRALGKSRPGAAELAAN
                     PRARSAIMRVAERTAAPTHHGGRA"
     gene            379..687
                     /gene="ftsL"
                     /locus_tag="PKN51_12280"
     CDS             379..687
                     /gene="ftsL"
                     /locus_tag="PKN51_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNT85918.1"
                     /translation="MTRLSIVLLVAVMVSALYVVRVQYDSRRLFTELDRASAEAHRLE
                     TERARLEVEKRAEATSLRVEKLARDKLAMRSITPAITEYVSSPLALPAASAPAAGERP
                     "
     gene            684..2420
                     /locus_tag="PKN51_12285"
     CDS             684..2420
                     /locus_tag="PKN51_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNT85919.1"
                     /translation="MRDIRYTSSPLLASKTPVWRSKFIVAMLALAFLVLIGRAAYVQV
                     FGNAFFQRQGQVRFARTLELPASRGRILDRNGLLLASSVVAPSIWAIPEDVDRDPARL
                     RELARLLSMPLAELNKKLSDEDKTFVWLKRQVDESVARQIAELKLAGIYQRKEYKRKY
                     PEGEAAAHVVGFANVENQGQEGAELSFEKRLAGRAGSRRVIKDRLGRVVEAVGEEVPP
                     VDGQDVQLSIDSKVQFFAYQKLRDAVTSNKARSGSVVVLDAQTGEVLALANYPSYSPD
                     NRRHLSGAQLRNIAVTDTFEPGSTMKPVTVAMALEAGKVTPQTLIATAPGSYKLDKFT
                     IRDTHNYGTLTVEGVVQKSSNVGSLKIAQRLSAQEMWETYTALGYGRKPELAFPGAAA
                     GRVRHWKSWRPVEQATMSYGYGLSASLFQMAHSYTAFSHDGRVIPATLLKAPEGASVE
                     GVRVFSEKNARAVRKMLALAAGPGGTGQRAQTVGYSVGGKSGTARKQVGKGYASNRYR
                     AWFTGMAPIEQPRVIVAVMIDEPSNGVYYGGAVAAPVFSEVVQQTLRIMGVQPDMAVK
                     PAIVAEQVEESF"
     gene            2422..3927
                     /locus_tag="PKN51_12290"
     CDS             2422..3927
                     /locus_tag="PKN51_12290"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNT85920.1"
                     /translation="MALAQCHHPTEAARWLRARVTGRLHADSRQVEPGDGFIAWPGAA
                     VDGRRFVAAALAQGARACLVEHEGVQAFGFEQPQVASYAHLKADTGPIAAAYYGEPSQ
                     ALQVVAVTGTNGKTSTAWWTSWALSKTELPTLSPCALVGTLGVGLPQALQVTGMTTPD
                     PVLLQRNFRELVDAGVRACAIEASSIGIAERRLDGTAIRVAVFTNFTQDHLDYHGSMQ
                     AYWQAKAALFDWPGLQAAVIHADDPRAGELVAKAQARGLDVWTVARERPARLRATDIA
                     YADAGLHWVVCEGEQRAALRTTLVGEYNIDNLMGVLATLRALGVPLQQAVAACTDLPA
                     VPGRMERIGLPGAPLAVVDYAHTPDAIDKALRALRPLAAQRGGRLWCVFGCGGNRDAS
                     KRPLMAQAAEAAADRLVVTSDNPRHEDPQAIIDAIGAGLRRPGAARFEVDRAQAIAAA
                     LTEAQPADVVLIAGKGHEDYQEVRGQRTPFSDQAVARRVLQRRVATGEAPA"
     gene            3924..5333
                     /gene="murF"
                     /locus_tag="PKN51_12295"
     CDS             3924..5333
                     /gene="murF"
                     /locus_tag="PKN51_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNT85921.1"
                     /translation="MSAMALSLQELAGWLTGARLVGDGAVRCARVHSDTRSLQAGDLF
                     VALKGERFDAASFLGEAAAQGAVAALCDERAEARLVAAGLPGVLVPDVRLALGELAHR
                     WRRRFDGPLIAVTGSNGKTTVTQMVASIFRAWQGTAALSTEGNLNNDIGLPLTLLRLR
                     PQHRVAVVELGMNHPGEIAQLAAIAAPTVALVNNAQREHQEFMASVEAVARENGAVLT
                     ALPADGVAVFPAADAFSPLWRSLAGGRRVLDFGAGAAVHLSQATWAVDHWAVSVATPV
                     GQFDGALHVAGRHNLHNALAATACALAAGAPLAAIARGLGEFRPVKGRSRTVLLRQRG
                     RAITLVDDTYNANPDSVRAVIDVLAELPGPRLLVLGDMGEVGEQGPAFHAEVGAYARQ
                     RGVEHLLAHGPQAIHAAMAFGGGRHFDDIGALIDAVRTHLPQCATLAVKGSRFMRMER
                     VVEALSAATGPTAEDAHAA"
     gene            5323..>6259
                     /gene="mraY"
                     /locus_tag="PKN51_12300"
     CDS             5323..>6259
                     /gene="mraY"
                     /locus_tag="PKN51_12300"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNT85922.1"
                     /translation="MLPSLATWLQTLSPDFGFFRVFQYLTFRALMAAMTALVIGIALG
                     PWVIRRLTALKIGQPVRGYAMQSHLVKSGTPTMGGVLILLSIAVSTLLWFDWSNRFVW
                     IVMLVTFGFGAIGWVDDWRKVVNKDPEGMRSREKYFWQSVIGLVAAFYLVFSISEASN
                     ARVWALFVNWVSSGFSIDLPPAAGLLVPFFKEISYPLGVLGFVILSYLVIVGSSNAVN
                     LTDGLDGLAIMPVILVGSALGVFAYVTGNAVYARYLLIPHIPGTGELLIFCAALAGAG
                     LAFLWFNAHPAEVFMGDVGALALGGALGTIAVIVRQ"
BASE COUNT          899 a         2143 c         2267 g          950 t
ORIGIN      
        1 cgcggtcaaa acccgcgagc cgggccagaa ccctgcaacg cgcacatttc aggctgttcg
       61 gattttcgtc aacgccgagc ttgaagagct cgaacaggcg ctagaggccg ctgtgcaggt
      121 gctgcaaccc gggggccggc tggtggtgat cagcttccac tcgctggaag accgcatcgt
      181 caagcagttc atcgcacgcc acgcgcgcgc gccggtggat cgccgcgcgc cgtttgccga
      241 accggcgccc ccacggttgc gcgcgctggg caaatcgcgc cccggtgcag ccgaactggc
      301 cgccaacccg cgcgcccgca gcgccatcat gcgggtggcc gagcgcaccg cggcgcccac
      361 gcatcacggg ggccgggcat gacgcgcctg tccatcgtgc tgctggtggc cgtcatggtg
      421 tcggcgctgt acgtggtgcg cgtgcagtac gactcgcgcc gcctgttcac cgagctcgac
      481 cgggccagcg ccgaggcgca tcgtctggag accgagcgcg cccgactgga ggtggaaaag
      541 cgcgccgagg ccacctcgct gcgcgtcgag aagctggcgc gcgacaagct ggccatgcgc
      601 agcatcacgc cggccatcac cgagtacgtg agctcgccgc tggcgctgcc ggcggcttcg
      661 gcgcctgcgg cgggggagcg gccatgaggg atatccgcta cacctccagc ccgctgctgg
      721 cgtccaagac gccggtgtgg cgcagcaagt tcatcgtcgc catgctggcg ctggccttcc
      781 tggtgctgat cgggcgcgcc gcctatgtgc aggtgttcgg caacgccttc tttcagcgcc
      841 agggtcaggt gcgctttgcc cgcaccctgg agctgccggc cagccgcgga cgcatcctcg
      901 atcgcaacgg cctgctgctg gcgtccagcg tggtggcgcc cagcatctgg gcgattccgg
      961 aggacgtgga tcgcgacccg gccaggctgc gcgagctggc gcgcctgttg agcatgccgc
     1021 tggctgagct gaacaagaag ctgtccgatg aggacaagac cttcgtctgg ctcaagcgcc
     1081 aggtggacga gtcggtggcg cggcagatcg ccgagctcaa gctggccggc atctatcagc
     1141 gcaaggaata caagcgcaag tacccggagg gcgaagccgc cgcccacgtg gtgggttttg
     1201 ccaacgtgga gaaccagggg caggagggcg ccgagctgtc gttcgaaaag cgcctggccg
     1261 ggcgcgccgg ctcgcgccgc gtcatcaagg accggctggg acgcgtggtc gaagcggtgg
     1321 gcgaggaagt cccgccggtg gacggtcagg acgtgcagct gtcgatcgac tccaaggtgc
     1381 agttcttcgc ctatcagaag ctgcgcgacg ccgtcacatc gaacaaggcc aggtcgggca
     1441 gcgtggtggt gctcgacgcc cagaccggcg aagtgctggc gctggccaac taccccagct
     1501 attcgcccga caaccgtcgc cacctgagcg gggcgcagct gcgcaacatc gccgtcaccg
     1561 acaccttcga gcccggctcg accatgaagc cggtcaccgt ggccatggcg ctggaggccg
     1621 gcaaggtgac gccgcagacg ttgatcgcca ccgcgccggg cagctacaag ctggacaagt
     1681 tcaccatccg cgacacccac aactacggca ccctgaccgt cgagggcgtg gtgcagaagt
     1741 ccagcaacgt cggctcgctg aagatcgccc agcgcctgag cgcgcaggaa atgtgggaaa
     1801 cctacaccgc gctcggttat ggccgcaagc cggagctggc ctttcccggc gcggcggcag
     1861 gccgggtgcg tcactggaag agctggcgcc cggtggaaca ggccaccatg tcctatggct
     1921 acggcctgtc ggcgtcgctg ttccagatgg cgcattccta caccgcgttc tcgcacgacg
     1981 ggcgggtgat tccggccacg ctgctgaaag cgcccgaagg cgccagcgtg gagggggtgc
     2041 gcgtgttttc cgaaaagaac gcgcgcgccg tgcgcaagat gttggcgttg gccgccggcc
     2101 ccggtggcac tggccaacgg gcgcagaccg tcggctattc ggtaggcggc aagtcgggca
     2161 cggcgcgcaa gcaggtcggc aagggttatg ccagcaaccg gtaccgcgcc tggttcaccg
     2221 gcatggcgcc gatcgagcag ccgcgcgtca tcgtcgccgt gatgatcgac gagcccagca
     2281 acggcgtgta ctacggcggc gccgtggcgg cgccggtgtt cagcgaagtg gtgcagcaga
     2341 cgctgcgcat catgggcgtg cagcccgaca tggcggtcaa gcccgccatc gtggccgagc
     2401 aggtcgagga gtcgttctga catggccctt gcccagtgcc accacccgac cgaagccgcc
     2461 cgctggctgc gtgcccgcgt gacgggtcgg ctgcacgccg acagccgcca ggtggagccc
     2521 ggcgacggct tcatcgcctg gccgggagcg gccgtggacg gccggcggtt cgtggccgcg
     2581 gcgctggcac agggtgcacg cgcctgcctg gtcgaacacg agggggtgca ggcgttcggc
     2641 ttcgaacagc cacaggtggc cagctacgcg cacctgaagg ccgacaccgg tccgatcgca
     2701 gcggcctact acggcgagcc gtcgcaggcg ctccaggtgg tggcggtgac cggcaccaac
     2761 ggcaagacct cgaccgcctg gtggaccagc tgggcgctat caaaaacaga gcttccgacg
     2821 ctttccccgt gcgcgctggt gggtactttg ggtgtgggtc tgccgcaggc gctgcaggtc
     2881 accggcatga ccacgcccga cccggtgctg ctgcagcgga actttcgtga gctggtcgac
     2941 gcgggggtgc gcgcctgcgc catcgaggcc tcgtccatcg gcattgcgga gcggcgcctg
     3001 gacggcaccg cgatccgcgt ggcggtgttc accaacttca cccaggacca cctcgactac
     3061 cacggcagca tgcaggccta ctggcaggcc aaggctgcgc tgttcgactg gccgggtctg
     3121 caggcggcgg tgatccacgc cgacgacccg cgggccggcg aactggtggc caaggcacag
     3181 gcgcgcgggc tggacgtctg gaccgtggcg cgggagcgcc cggcgcgcct gcgcgccacc
     3241 gacatcgcct atgccgacgc aggtctgcac tgggtggtgt gcgagggcga gcagcgcgcc
     3301 gccctgcgca ccactttggt gggcgagtac aacatcgaca acctgatggg cgtgctggcc
     3361 acgctgcggg cgctgggcgt gccgctgcag caggccgtgg ccgcctgcac cgacctgcct
     3421 gcggtgccgg ggcgcatgga gcgcatcggc ctgcccggtg cgccgctggc ggtggtcgat
     3481 tacgcccaca cgccggatgc catcgacaag gccctgcgcg ccctgcgacc gctggcggcg
     3541 cagcgcggtg ggcgcctgtg gtgcgtgttc ggctgcggcg gcaaccgcga cgccagcaag
     3601 cgcccgctga tggcgcaggc ggccgaggcg gcggccgatc ggctggtggt gaccagcgac
     3661 aacccgcgcc acgaggaccc gcaggccatc atcgacgcca tcggcgccgg cctgcgccgc
     3721 ccgggcgcgg cacggttcga ggtggaccgc gcccaggcca ttgcggcggc cctgaccgag
     3781 gcgcagccgg ccgacgtggt gctgatcgcc ggcaagggcc acgaggacta tcaggaggtg
     3841 cgcgggcaac gcacgccgtt ctccgaccag gccgtggcgc gccgggtgct gcagcggcgc
     3901 gtcgccacgg gggaggcgcc ggcatgagtg ccatggcact gagcctgcag gagctggccg
     3961 gctggctgac cggcgcacgc ctggtcggcg acggcgccgt gcgctgcgcg cgcgtgcatt
     4021 ccgacacccg cagcctgcag gccggtgacc tgttcgtggc gctcaagggc gagcgttttg
     4081 atgccgcgag cttcctgggc gaggcggcgg cgcagggggc ggtggcggcg ttgtgcgacg
     4141 agcgcgccga ggcccggctg gtggccgccg gtctgccggg cgtgctggtg cccgacgtcc
     4201 gccttgccct gggcgagctg gcgcaccggt ggcgccgccg tttcgacggc ccgctgatcg
     4261 ccgtcaccgg cagcaacggc aagaccacgg tgacgcagat ggtcgcctcg atcttccgcg
     4321 cctggcaggg gacagccgcg ctgtcgaccg aaggcaacct gaacaacgac attggactgc
     4381 cgctgaccct gctgcgcctg cgcccgcagc accgggtggc ggtggtggag ctgggcatga
     4441 accatcccgg cgagattgcg caactggcgg ccatcgccgc acccaccgtg gcgctggtca
     4501 acaacgccca acgtgagcac caggaattca tggccagcgt ggaggcggtg gcgcgcgaaa
     4561 acggcgccgt gctgaccgcg ctgccggccg atggcgtggc ggtgttcccg gcggccgatg
     4621 cgttcagtcc gctctggcgc tcactggccg gtggccgtcg tgtgctcgac tttggcgccg
     4681 gcgctgccgt gcacctgtcc caggccacct gggcggtcga ccactgggcg gtgagcgtcg
     4741 ccacgccggt cggccagttc gacggcgccc tgcatgtggc gggccgtcac aacctgcaca
     4801 acgcgctggc agccaccgcc tgtgcgctgg ccgccggggc accgctggcg gccatcgccc
     4861 gcggtctggg cgagttccgc ccggtcaagg gccgttcgcg caccgtgctg ctgcgccagc
     4921 gtggtcgtgc catcaccctg gtcgacgaca cctacaacgc caacccggac tcggtgcgcg
     4981 ccgtgatcga cgtgctggcc gagctgcccg gcccgcgcct gctggtgctg ggcgacatgg
     5041 gcgaggtggg cgagcagggc ccggccttcc acgccgaagt cggcgcctac gcccgccagc
     5101 gcggcgtgga gcacctgctg gcgcacgggc cgcaggccat ccacgccgcc atggccttcg
     5161 ggggagggcg gcatttcgac gacatcggcg cgctcatcga cgccgtgcgc acccacctgc
     5221 cgcagtgcgc cacgctggcg gtcaaggggt cgcgcttcat gcgcatggaa cgcgtggtgg
     5281 aggcgctgtc ggccgccacc ggcccaaccg cggaggacgc ccatgctgcc tagcctggcc
     5341 acctggttgc agacgctgtc gccggacttc ggcttcttcc gcgtgttcca gtacctcacc
     5401 ttccgcgcgc tgatggcggc catgacggcg ctggtgatcg gcattgcgct gggcccctgg
     5461 gtgatccgcc gtctgacggc gctgaagatc ggccagccgg tgcgcggcta cgccatgcag
     5521 tcgcacctgg tcaagagtgg cacgcccacc atgggcgggg tgctgatcct gctgtcgatc
     5581 gccgtctcca cgctgctgtg gttcgactgg tccaaccgct tcgtgtggat cgtgatgctg
     5641 gtcacctttg gctttggtgc catcggctgg gtggacgact ggcgcaaggt ggtcaacaag
     5701 gacccggaag gcatgcgctc gcgcgagaag tacttctggc agtcggtgat cggtctggtc
     5761 gctgccttct acctggtgtt cagcatttcc gaggcgtcca acgcgcgcgt ctgggcgctg
     5821 ttcgtcaact gggtgagttc cggcttcagc atcgacctgc caccggccgc cggtctgctg
     5881 gtgccgttct tcaaggagat cagctacccg ctgggggtgc tgggcttcgt gatcctgagc
     5941 tacctggtga tcgtcggctc cagcaacgcc gtcaacctga ccgacgggct ggatggcctg
     6001 gccatcatgc cggtgatcct ggtcggttcg gcactgggcg tgtttgccta cgtcaccggc
     6061 aacgccgtgt atgcgcgcta cctgctgatc ccgcacattc cgggcacggg cgaactgctg
     6121 atcttctgcg ccgcactggc cggggccgga ctggccttcc tgtggttcaa cgcccacccg
     6181 gcggaggtct tcatgggcga tgtcggcgcg ctggcgctgg gcggggcgct gggcaccatc
     6241 gccgtcatcg tgcggcagg
//
LOCUS       DAOQTZ010000137         6237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_13864_length_6237_cov_12.884989, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000137 DAOQTZ010000000
VERSION     DAOQTZ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13864_length_6237_cov_12.884989"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..554
                     /gene="trxA"
                     /locus_tag="PKN51_12305"
     CDS             222..554
                     /gene="trxA"
                     /locus_tag="PKN51_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNT85923.1"
                     /translation="MASDLIKHVSDSSFEADVLKSDKPVLVDFWAEWCGPCRAIAPTL
                     DELAGAYDGKLQIAKINVDDNRAVPAKFGIRGIPTLMVFKNGQLAATKVGALTKAQLT
                     QFIDQQLA"
     gene            801..2066
                     /gene="rho"
                     /locus_tag="PKN51_12310"
     CDS             801..2066
                     /gene="rho"
                     /locus_tag="PKN51_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNT85924.1"
                     /translation="MHLNELKVLHVSELLKQAEALEIDNASRMRKQELMFAIIKKRAK
                     GGEQVFADGVLEILPDGFGFLRSPDTSYTASTDDIYISPSQVRRFNLHTGDMIEGEVR
                     IPKDGERYFALTKLDAVNGDAPEKNKHKVMFENLTPLFPREQMRLERDNFKGDENITG
                     RIIDVIAPIGKGQRALIVAPPKSGKTVMMQTIAHAISANYPDSHMMVLLVDERPEEVT
                     DMQRSVKGEVIASTFDEPAARHVHVAEMVIERAKRLVELKKDVVILLDSITRLARAYN
                     NVVPSSGKVLTGGVDANALHRPKRFLGAARNVEEGGSLTIIATALIDTGSRMDEVIFE
                     EFKGTGNSEIHLDRRLYEKRVFPSIQLNRSGTRREELLLAPEVLQKTRILRQFMYNMD
                     EIEAMEMVIKSMKATKTNVEFFDMMRRGG"
     gene            2146..2400
                     /locus_tag="PKN51_12315"
     CDS             2146..2400
                     /locus_tag="PKN51_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNT85925.1"
                     /translation="MREGIHPNYREVCFVDLSNGFKFVTRSCVNTKETVKMDDGRELP
                     LFKLDTSSESHPFYTGTQKSVDNMGGRVEKFRNRYGKVAR"
     gene            2489..4198
                     /locus_tag="PKN51_12320"
     CDS             2489..4198
                     /locus_tag="PKN51_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85926.1"
                     /translation="MNRPSPAIVTQDAVRRLPRWALLLLCVAYVIPGYVGRAPWKNAD
                     LASFGYMLALRDAGDGQAWLHPTLMGVAPDSPALLPYWLGAWAMRWLQPLGLAPDVAV
                     RLPFVLLLAGTLVASWSAMYLLARHPSAQPVAFAFGGEAEPRDYARALADGSLLALIA
                     TLGLAQFSHETTPVLAQLFFGTLVFYGLAALATRPRSAGLALACGLPGLTLSGAPTTA
                     LLYAGLSALALALPGPTHVPAAVRRGALWLLPALMAACLALALTLGLWPTELPAWRSR
                     WSDWRSLGRLLLWFTWPAWPLVLWTLWRWRRQLLAPRQQRHLGLPLLIASVPLLSAVF
                     TLAGDRALLLALPALAALAALALPTFSRGAAALIDWFTVLFFSGWAIVIWVVWLAMQT
                     GVPAKPAANVARLAPGFEAPFQWPALVAALAGTLAWVALARWRTGRHRTALWKSLVLP
                     AAGATLCWLLLMTLWLPALDYGRSFAPQMREVRRWVGDAPCVQVHGLGTPQLVGVGFH
                     GGWPTVAAQGPARCPWLLVDVDAQASLPAAVHLHEWQLLTRIRRPTDDNESLLVFRRV
                     DGL"
     gene            4254..5285
                     /locus_tag="PKN51_12325"
     CDS             4254..5285
                     /locus_tag="PKN51_12325"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HNT85927.1"
                     /translation="MPDLAYPDPTHWPGRAAPSAAPADDRWAVIDLGSNSFRLEIVRL
                     TDGQFERLDYLKETVRQGGGLGPDGRLSAQALQAGWDCLARFGQALQGFAPWRVRAIA
                     TQTLREARNGDEFLARGQRLLGFPIEVISGQEEARLIYRGVVQRLPASHERRLVLDVG
                     GRSTELVVGDGPQPRVMTSRLLGSVAWSMAHFGDQRLSAAQFERAEAAARTVLADAVA
                     SFGRPHWDAAYGCSGTVGAVADALRLGGLVTRRHRVTRAGLLQLRAQLIAAGDSRTLQ
                     LPGIKPERRAVIGGGVSVLCALFALLDIDRLRYVPGALRHGALHDWLERPQAGQARSA
                     APAARSARR"
     gene            complement(5351..6100)
                     /locus_tag="PKN51_12330"
     CDS             complement(5351..6100)
                     /locus_tag="PKN51_12330"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNT85928.1"
                     /translation="MSLAPERSLGFAGASNFRDLGGYAGAGGLPVRWRRLFRSDHLAG
                     LTPKDAQQFQRLGVTRVFDFRGEQERAAVPYDLPGVTQHALTIEPTVVQRMKDLLDAG
                     EAITPERTVALMQQTYRGFVHDNADRFAELFAHLLADDAPLVFHCTAGKDRTGFAAAL
                     ILLALDVPRPVVMHDYLLTNAFYRMPAAHDSRAPREVLQVLWRVQEDFLNAALDIVEQ
                     EDGGVRGYLSRQLRVGEAERQRLQALYLAGA"
BASE COUNT          939 a         2194 c         2136 g          968 t
ORIGIN      
        1 gcgcgccggg tgcgccgcca gctggtgcaa ttgctggcgc acctcggcca gcagcgcgcg
       61 ccagggcgcc ggcgcggcag cggcgcccag gggttcatcg ccaacagcta tggcattcat
      121 agcgatcggc gatgccgcgc ggcaaacggg ggcatggggc tttctgtcac aatccgggct
      181 actttatccg cttggatcac cccgtttctc aaggatgccc gatggccagt gacctgatca
      241 aacacgtgag cgacagctcc ttcgaagccg acgtcctgaa gtccgacaag ccggtgctgg
      301 tggacttctg ggccgaatgg tgcggcccct gccgcgccat cgcccccacg ctggacgagc
      361 tggccggcgc ctacgacggc aagctgcaga tcgccaagat caacgtcgac gacaaccgcg
      421 ccgtgccggc caagttcggc atccgcggca ttcccacgct gatggtgttc aagaacggcc
      481 agctggcggc caccaaggtg ggcgcgctga ccaaggcgca actgacgcag ttcatcgacc
      541 agcaactggc ctgagcggcc gtcggcagcc ggcggcgccc acgccccggc cgacggcgcg
      601 atttttcctg ccataatccc tcctcgaaag cggacccaca ggtaagggtt cgccggtgcc
      661 ccggtcacgg ggcgcccgtt ccggagcctc gtttctgctg gctccgacag cctccccagc
      721 ttcctcgcca tttttcagcc ccccttggtc tggccgatcc acattccagg atcgatgtcc
      781 accgcaagac gccgattgcc atgcacctga acgaactcaa agtactgcac gtgtccgaac
      841 tgctcaagca ggccgaggcg ctggagatcg acaacgccag ccgcatgcgc aagcaggagc
      901 tgatgttcgc catcatcaaa aagcgcgcca agggcggcga gcaggtgttt gccgacggcg
      961 tgctggagat cctgcccgac ggcttcggct ttctgcgcag ccccgacacc agctacaccg
     1021 ccagcaccga cgacatctac atcagcccca gccaggtgcg ccgcttcaac ctgcacaccg
     1081 gcgacatgat cgagggcgag gtgcgcattc ccaaggatgg cgagcgctat ttcgccctga
     1141 ccaagctgga cgccgtcaac ggcgacgcgc ccgagaagaa caagcacaag gtgatgttcg
     1201 agaacctcac cccgctgttc ccgcgcgagc agatgcggct ggagcgtgac aacttcaaag
     1261 gcgacgagaa catcaccggc cgcatcatcg acgtcatcgc gcccatcggc aagggccagc
     1321 gtgcgttgat cgtggcgccg cccaagagcg gcaagacggt gatgatgcag accatcgcgc
     1381 acgccatcag cgccaactac ccggacagcc acatgatggt gctgctggtc gacgaacgcc
     1441 ccgaggaagt gaccgacatg cagcgctcgg tcaagggcga ggtgattgcc tccaccttcg
     1501 acgagccggc ggcgcgccac gtgcatgtgg ccgagatggt gatcgagcgc gccaagcgcc
     1561 tggtcgagct caagaaagac gtggtgatcc tgctcgactc catcacccgc ctggcacgcg
     1621 cctacaacaa cgtggtgccc tccagcggca aggtgctgac cggcggcgtc gacgccaacg
     1681 ccctgcaccg ccccaagcgc tttctgggcg cggcgcgcaa cgtcgaggaa ggcggcagcc
     1741 tgaccatcat cgccaccgcc ctgatcgata ccggcagccg catggacgag gtgatcttcg
     1801 aggaattcaa gggcaccggc aacagcgaaa tccacctgga ccgccgcctg tacgaaaagc
     1861 gcgtgttccc cagcatccag ctcaaccgct cgggcacgcg gcgcgaggag ctgctgctgg
     1921 cgcccgaggt gctgcaaaag acccgcatcc tgcgccagtt catgtacaac atggacgaga
     1981 tcgaggcgat ggagatggtc atcaagagca tgaaggccac caagaccaac gtcgagttct
     2041 tcgacatgat gcggcgcggc ggctgaaccc ctataattgc cggtttcgcg gaaagtacgc
     2101 ccgatgttcg ggcgcggctg ccgtaaccac ctcaaggaat tcatcatgcg tgaaggcatt
     2161 caccccaact accgcgaagt gtgcttcgtg gacctgtcca acggcttcaa gttcgtcacc
     2221 cgctcctgcg tcaacaccaa ggaaacggtg aagatggacg acggtcgcga gctgccgctg
     2281 ttcaagctcg acacctccag cgaatcgcac cccttctaca ccggcaccca gaagtcggtc
     2341 gacaacatgg gcggtcgcgt cgagaagttc cgcaaccgct acggcaaggt cgcccgctga
     2401 cgtcacacgg cgccgcactc tggccagcca gggcagcacg ggcaaccgcg ctgccctttt
     2461 gtttggccgg ccgccgccct gcccgccctt gaaccgcccc agccccgcca tcgtcaccca
     2521 ggacgcggtc cggcgcctgc cgcgctgggc gctgctgctg ctgtgcgtgg cctatgtgat
     2581 tcccggctac gtgggccgcg cgccgtggaa gaacgccgat ctggcctcgt tcggctacat
     2641 gctggcactg cgcgacgccg gcgacgggca ggcgtggctg caccccacgc tgatgggcgt
     2701 ggcgcccgac agcccggccc tgctgcccta ctggctgggc gcctgggcga tgcgctggct
     2761 gcagccgctc ggcctggccc ccgacgtcgc cgtgcgcctg ccgtttgtgc tgctgctggc
     2821 cggcaccctg gtggccagtt ggtcggccat gtacctgctg gcacgccacc ccagcgcgca
     2881 gccggtggcc tttgccttcg gtggcgaagc cgagccgcgc gactacgcgc gtgcgctggc
     2941 cgacggcagc ctgctggcgc tgatcgccac cctggggctg gcgcagtttt cgcacgaaac
     3001 cacgccggtg ctggcgcagc tgttctttgg caccctggtg ttctacgggc tggcggcgct
     3061 ggcgacccgc ccccggagcg ccggtctggc gctggcctgc ggcctgcccg ggctgacgct
     3121 gtcgggcgcc cccaccaccg ccctgctgta cgccggactg agcgcgctgg cgctggcgct
     3181 gcccggcccc acccatgtgc cggcggcggt gcggcgcggc gccctgtggc tgctgccggc
     3241 gctgatggcc gcctgcctgg cgctggcgct caccctggga ctgtggccca ccgagctgcc
     3301 ggcctggcgc agccgctgga gcgactggcg ctcgctcggc cgcctgttgc tgtggttcac
     3361 ctggccggcc tggccgctgg tgctgtggac cctgtggcgc tggcgccgcc agttgctggc
     3421 accgcgccag cagcgccacc tggggctgcc gctgctgatc gccagcgtgc cgctgctgag
     3481 tgcggtgttc accctggccg gcgaccgggc gctgttgctg gcgctgccgg cgctggcggc
     3541 gctggcggcg ctggcgctgc ccaccttcag ccgcggtgcg gcagcgctga tcgactggtt
     3601 cacggtgctg ttcttcagcg gttgggcgat cgtgatctgg gtggtgtggc tggccatgca
     3661 gaccggcgtg ccggccaagc cggcggccaa cgtggcgcgg ctggcgccag gcttcgaagc
     3721 gcccttccag tggccggccc tggtcgccgc gttggccggc acgctggcct gggtggcgct
     3781 ggcgcgctgg cgcaccggtc ggcatcgcac cgcgctgtgg aagagcctgg tgctgccggc
     3841 cgccggcgcc accctgtgct ggctgctgct gatgacgctg tggctgccgg cgctggacta
     3901 cggccgcagc ttcgcgccgc aaatgcgcga agtgcgccgc tgggtgggcg acgccccctg
     3961 cgtgcaggtg catggactgg gcacgccgca gctggtgggc gtgggatttc atggcggctg
     4021 gccgaccgtg gccgcgcagg gcccggcgcg ctgcccctgg ctgctggtgg acgtggacgc
     4081 ccaggccagc ctgccagcgg cagtgcacct gcacgagtgg cagctgctga ctcgcatccg
     4141 gcgtcccacg gacgacaatg aaagcctgct ggtgtttcgc cgtgtggatg gcctgtaagc
     4201 tatcatttac atagctgctg gcgctttctg caattgccct ggacgtcgtt ttcatgcctg
     4261 acctggctta ccctgatccg acccactggc ccggccgggc ggcgccaagc gccgcgccgg
     4321 cggacgaccg ctgggcggtg atcgatctgg gctccaacag cttccggctg gagatcgtgc
     4381 gcctgaccga cgggcagttc gagcgcctcg actacctcaa ggaaaccgtg cgccagggtg
     4441 gcgggctggg gcccgacggc cgcctgtcgg cgcaggcgct gcaggccggc tgggactgcc
     4501 tggcgcgttt tggccaggcc ctgcaggggt ttgcgccctg gcgggtgcgc gccatcgcca
     4561 cccagaccct gcgcgaggcc cgcaacggcg acgaattcct ggcccgcggc cagcggttgc
     4621 tgggctttcc catcgaagtc atcagcgggc aggaagaagc ccgcctgatc taccgcggcg
     4681 tggtgcagcg cctgccggcg tcgcacgagc ggcgcctggt gctggacgtc ggcgggcgct
     4741 cgaccgagct ggtggtcggt gacgggccgc agccgcgcgt catgacttcc cgcctgctgg
     4801 gcagcgtggc ctggtcgatg gcgcatttcg gcgatcagcg gctgtcggcc gcacagttcg
     4861 agcgcgccga ggccgccgcc cggacggtgc tggccgatgc cgtggccagc ttcggccggc
     4921 cgcactggga tgccgcctac ggctgctccg gcaccgtggg cgcggtggcc gacgccttgc
     4981 gcctgggcgg cctggtgacc cggcggcacc gggtcacgcg cgccggcctg ctgcagttgc
     5041 gggcgcagct gatcgccgcc ggcgacagtc gcacgctgca gctgcccggc atcaagcccg
     5101 agcgccgggc cgtgatcggt ggcggtgtgt cggtgctgtg cgcgctgttc gcgctgctgg
     5161 acatcgaccg gctgcgctac gtgcccggcg ccctgcgcca cggcgcgctg cacgactggc
     5221 tggagcgccc gcaggcgggc caggcacgca gcgccgcccc ggcggcccgc agcgcgcgcc
     5281 gctgacgcca gccccgtcgc tcacgcggat tcgcgttcaa ctgccgtttc cagaataaag
     5341 caggttcgca tcaggcaccc gccaggtaca gcgcctgcag tcgctggcgc tcggcctcac
     5401 ccacgcgcaa ctggcgcgac aggtagccgc gcacaccgcc gtcttcctgc tcgacgatgt
     5461 cgagcgcggc gttcagaaag tcttcctgca cgcgccacag cacctgcagc acctcgcgcg
     5521 gggcgcggct gtcgtgcgcg gcgggcatgc ggtagaacgc attggtcagc aggtagtcgt
     5581 gcatcaccac cgggcgcggc acgtccagcg ccagcaggat cagggcggcg gcaaagccgg
     5641 tgcggtcctt gccggcggtg cagtgaaaca ccagcggcgc gtcgtcggcc agcagatggg
     5701 cgaacagttc ggcaaagcgg tcggcgttgt cgtgcacgaa gccgcggtag gtctgctgca
     5761 tcagcgccac cgtgcgctcg ggggtgatgg cctcgcccgc gtccagcagg tccttcatgc
     5821 gctgcaccac ggtgggttcg atggtcagcg cgtgctgcgt cacgccgggc aggtcgtagg
     5881 gcacggcggc gcgttcctgc tcgccgcgga agtcgaacac gcgcgtgacg cccaggcgct
     5941 ggaactgctg cgcatccttc ggcgtcagcc cggccagatg gtcggagcga aacaacctgc
     6001 gccagcgcac cggcagaccg cccgcgccgg cgtagccgcc caggtcgcga aagttgctgg
     6061 caccggcaaa gccaagagat cgttcgggcg cgagggacat gggcgaggca tccacagaaa
     6121 ccagcctcga ttgtcggtca cgggcggacg ccgcgcatga cacgggtgtg accgaggcct
     6181 gaggcgacgc gctcagccta gactcagcct agaaacggca gttgaccgct tgtcaac
//
LOCUS       DAOQTZ010000138         6114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_14249_length_6114_cov_13.431589, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000138 DAOQTZ010000000
VERSION     DAOQTZ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14249_length_6114_cov_13.431589"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..822
                     /gene="greB"
                     /locus_tag="PKN51_12335"
     CDS             262..822
                     /gene="greB"
                     /locus_tag="PKN51_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNT85929.1"
                     /translation="MSKAFTKEQDGDDDDEVALPPLPAGGRNYITPQGYARLRGELLS
                     LIDDERPRVVETVHWAAKNGDRSENGDYLYGKKRLREIDRRIRFLTRRLEIAEITDPS
                     LHHGGEQVFFGATVTYVDQDGAERSVTIKGIDEAEGARGEVSWISPIARTLLRARVGD
                     VLSLPTPAGVQEIEVLAVRYPAPSAQ"
     gene            complement(955..3186)
                     /locus_tag="PKN51_12340"
     CDS             complement(955..3186)
                     /locus_tag="PKN51_12340"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNT85930.1"
                     /translation="MPAAEVPTTAAANAAAASFAALEARLDYLDAADLERVRQAYRYA
                     DTAHLGQMRHSGEPYITHPIAVAAQVAEWKLDASALMAALLHDTLEDCGVSKADLLER
                     FGAQVAELVDGLTKLDKIQFSTREEGQAESFRKMLLAMARDVRVILVKLADRLHNMRT
                     MGDMPRSKWGRIATETLEIYAPIAHRLGLNQTYRELQDLSFRHLKPWRYSVLAKALAR
                     ARQRRGDLMQKLQHDLQAALADVGLNARIVGREKTLYSLYRKMDHKRQSFAQVNDLYG
                     VRIILPRVLDCYTGLGVLHQMFKPVPGRFKDYIAIPKVNGYQSLHTTLVGPASINIEF
                     QLRTEPMDLVAEAGVAAHWLYKAGLAGHTPDGELGTHWLQSLLDIQNETRDAAEFWDH
                     IKVDLFPDSVYVFTPRGQIMALPQGATTVDFAYAIHSNVGDHTVAAKVNNEQVPLRTE
                     LRNGDVVEIITAPVSRPNPAWLGFVRTGRARSRIRSHLKSLAQDESRVLGEKLLAQAL
                     RAEGLSQLPGSADGDKPLWDKLLRFTGNRSQEEMYADIGLGKRSASMVAKRLVTLLTA
                     EGAKPDALLLTRERFTAHENLSQGAVLLDGAENASVKYATCCRPIPGDGVVGYLGRGE
                     GLVVHTDDCPVARRLQNKDAERFITVAWADEPVRAFEVGIVVTVVNGKGVLARVAAAI
                     AAAEADIVHMGMAEESAQDALDLRFVISVRDREHLDAVLRSLRRTQSVLRANRGPTST
                     SGG"
     gene            complement(3314..3514)
                     /gene="rpoZ"
                     /locus_tag="PKN51_12345"
     CDS             complement(3314..3514)
                     /gene="rpoZ"
                     /locus_tag="PKN51_12345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNT85931.1"
                     /translation="MARITAEDALEKIPNRFQLVLAATYRARMLNQGHAPKVESRNKA
                     GVTALREIAAGKIGLEMLKKVT"
     gene            complement(3539..4159)
                     /gene="gmk"
                     /locus_tag="PKN51_12350"
     CDS             complement(3539..4159)
                     /gene="gmk"
                     /locus_tag="PKN51_12350"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNT85932.1"
                     /translation="MDHPGNLFVVAAPSGSGKSSLVKALMEVDAGVQPSISHTTRAPR
                     GQDKHGREYYFVDDAEFDAKIAAGDFLEWAHVHGRRYGTSRQAIEARIAAGGDVVLEI
                     DWQGALQIQRQFPNAILVFVLPPSWEELRARLQRRGEDTPEVIELRLANAREELAQVK
                     HFDYVIINEVFERALFDLKTIVHAQRLRYTAQKTARAETFQALGID"
     gene            complement(4222..4857)
                     /locus_tag="PKN51_12355"
     CDS             complement(4222..4857)
                     /locus_tag="PKN51_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HNT85933.1"
                     /translation="MHSPTPDLPCSTAAERNRGPIWGVLRRLLAPSGHALEIASGTGQ
                     HAAHFAHALPGWSWQPSDVSDAGFATVQGWADRLQARNVRRPVRLDVLDSRWPSEGAP
                     FADGAFDLIFCANMLHISPWPTCAALMRGAARHLAPGGALVVYGPFIEADLPTAPSNH
                     AFDAELRRRHPSWGLRDLEAVRTEAQGAGLALAERHALPANNLLLVFRARE"
     gene            complement(4988..6004)
                     /locus_tag="PKN51_12360"
     CDS             complement(4988..6004)
                     /locus_tag="PKN51_12360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNT85934.1"
                     /translation="MNRNLSDIALSMLDLVPVREGRSVADALAVALQTAQHAERLGFT
                     RYWLAEHHNMAGIASSATAVLVGHIAGGTERIRVGSGGVMLPNHAPLVVAEAFGTLAE
                     LFPGRIDLGLGRAPGTDPLTMRALRRDRIETADDFPRDVAELQRLLDRPQPGQKLIAN
                     PGAGTRVPIWLLGSSLFSAQLAAERGLPYAFASHFAPGLLLQAIEVYRRGFRPSAVLD
                     KPYVMIGVPLVAAPTDAEAMFLASSIFARVLGILRGARGLLQPPQEGFLERLDAPARA
                     GIADFLACAVIGAPDTVRQGLQALADATDCDEMMFTCDIYEPALRLRALDIAMQVRQD
                     SAGA"
BASE COUNT          918 a         1971 c         2197 g         1028 t
ORIGIN      
        1 gcacttgcat ggggtgcaag gggtcgcgag ttcgaatccc gccacgccga ccaattaaat
       61 caaggactta gcttcgcaag gggctaagtc cttgttcctt ttttggctct tgggtgtccg
      121 ctgtacctcc cagctccagg gctacccgcc ggggttgggg atgacatcac tgatcaaccc
      181 ggtgagctcg caggtgctca agctttgccg gcaagggtct gcgggcgttg gcgacttgcg
      241 tggcggtcta tcatgcctcg catgagcaag gcgtttacca aggagcaaga cggcgatgac
      301 gacgacgagg tcgcgctgcc gcccttgccc gcaggcggac gcaactacat cacgccgcag
      361 ggctacgccc gcctgcgtgg cgagttgctg tcgctgatcg acgatgagcg gccccgggtg
      421 gtcgagacgg tgcattgggc cgccaagaac ggcgaccgca gcgagaacgg cgactacctg
      481 tacggcaaga agcggctgcg cgagatcgac cggcgcatcc gctttctgac gcgacggctg
      541 gagatcgccg agatcaccga ccccagcctg caccacggtg gcgagcaggt gttcttcggc
      601 gccaccgtca cctacgtcga tcaggacggg gccgagcgca gcgtcaccat caagggcatc
      661 gacgaagccg aaggagcgcg tggcgaggtg agctggatca gcccgatcgc gcgcaccctg
      721 ctgcgcgccc gtgtgggcga cgtgctgtcg ctgccgacgc cggccggcgt gcaggagatc
      781 gaagtgctgg cggtgcgcta tccggcccca tcggcgcagt gatcgagccg cagacgctat
      841 gaaaaacgta gcttctggcg cttgttggta aagcgctgga acatcaaacc atggtcatcc
      901 atggcagacc ctgcggcggt ctgcgcagtg gcctgggtgc gactcagcct aggctcagcc
      961 gccgctggtg gatgtcggac cgcggttggc gcgcagcacc gactgggtgc ggcgcaggct
     1021 gcgcagcacg gcgtccaggt gttcgcggtc gcgcacgctg atgacgaagc gcaggtccag
     1081 cgcgtcctgc gcggattctt cggccatgcc catgtggacg atgtcggcct cggcggcggc
     1141 gatggcggcc gccacgcggg ccagcacgcc tttgccgttg accacggtga cgacgatgcc
     1201 gacctcgaag gcgcgcaccg gctcatccgc ccaggcgacg gtgatgaagc gttcggcgtc
     1261 cttgttctgc agacggcgtg cgaccgggca gtcgtcggtg tgcaccacca ggccttcacc
     1321 gcggcccaga tagccgacca cgccgtcgcc cgggatgggg cggcagcagg tggcgtattt
     1381 gaccgaggcg ttttcggcgc cgtcgagcag caccgcgccc tgcgacaggt tctcgtgcgc
     1441 ggtgaagcgc tcgcgcgtga gcagcagcgc atcgggcttt gcgccctcgg ccgtcagcag
     1501 cgtgaccagg cgcttggcca ccatgctggc cgagcgcttg cccaggccga tgtcggcata
     1561 catctcctcc tgcgagcggt tgccggtaaa gcgcagcagc ttgtcccaca gcggtttgtc
     1621 gccgtcggcg ctgccgggca gctggctcag gccttcggcg cgcagggcct gggccagcag
     1681 tttttcgccc agcacgcgcg attcgtcctg cgccaggctc ttgagatggc tgcggatgcg
     1741 cgagcgcgca cgcccggtgc gcacgaagcc cagccaggcc gggttggggc gcgacaccgg
     1801 cgcggtgatg atttccacca cgtcgccatt tcgcagctcg gtgcgcagtg gcacctgctc
     1861 gttgttgacc ttggccgcca ccgtgtggtc gcccacgttg ctgtggatgg cgtaggcgaa
     1921 gtcgaccgtg gtggcgccct gcggcagcgc catgatctgg ccgcgcgggg tgaacacata
     1981 caccgaatcg ggaaacaggt cgaccttgat gtggtcccag aactcggcgg cgtcgcgcgt
     2041 ctcgttctgg atgtccagca gcgactgcag ccagtgcgtg cccagctcgc cgtcgggcgt
     2101 atggccggcc agcccggcct tgtagagcca gtgcgcggcc acgccggctt cggccaccag
     2161 atccatcggt tcggtgcgca gctgaaactc gatgttgatg ctggccggtc cgaccagggt
     2221 ggtgtgcagc gactggtagc cgttgacctt ggggatggcg atgtagtcct tgaagcgccc
     2281 cggcaccggc ttgaacatct ggtgcagtac gcccaggccg gtgtagcagt ccagcacgcg
     2341 cggcaggatg atgcgcacgc catacaggtc gttgacctgg gcgaacgact ggcgcttgtg
     2401 gtccatcttg cggtagaggg aatacagcgt cttctcgcgc ccgacgatgc gcgcgttcag
     2461 gcccacgtcg gccagcgcgg cctgcaggtc gtgctgcagc ttctgcatca ggtcgccgcg
     2521 gcgctggcgt gcgcgtgcca gcgccttggc cagcacgctg tagcgccagg gcttgaggtg
     2581 gcgaaacgac aggtcctgca gctcgcggta ggtctggttc aggcccaggc ggtgggcgat
     2641 gggggcgtag atttccagcg tttcggtggc gatgcggccc cacttgctgc gcggcatgtc
     2701 gcccatggtg cgcatgttgt gcaggcggtc ggccagcttg accaggatga cgcgcacgtc
     2761 gcgcgccatg gccagcagca tcttgcggaa ggactcggcc tgaccttcct cgcgggtgct
     2821 gaactggatc ttgtccagct tggtcagccc gtcgaccagc tcggccacct gggcgccgaa
     2881 gcgctccagc agatcggcct tgctgacgcc gcagtcttcc agcgtgtcgt gcagcagcgc
     2941 tgccatcagg gcgctggcgt ccagcttcca ttcggccacc tgggcggcca cggcgatcgg
     3001 gtgggtgatg tagggctcgc cgctgtggcg catctgcccg aggtgggcgg tgtcggcata
     3061 gcggtaggct tgacgcacgc gctccagatc ggccgcgtcc agatagtcca gtctggcttc
     3121 aagcgcagca aagctggccg cggccgcgtt ggcggcggcc gtggtgggca cttcggcagc
     3181 tggcatgacc gaactcatgc tcgtaattta accgccccgt gcgccatttg tgcaactgca
     3241 gcaaaaaggc gccccgcagg gcgccctgag ctgtggcgaa cgctggcacg gcgggttgcc
     3301 gtgcgcggtc ggttcacgtc actttcttga gcatttccag gccgatctta ccggcggcga
     3361 tctcgcgcag cgccgtcacg ccggccttgt tgcgcgattc caccttgggg gcatggccct
     3421 ggttgagcat gcgggcgcgg taggtggccg ccagcacgag ctggaagcgg ttggggattt
     3481 tttcgagggc gtcttcggcg gtgatgcgtg ccatggagcg tgagcggtgc gggtggaatc
     3541 agtcgatgcc gagggcttga aaagtctcgg cgcgggcggt tttctgggcc gtgtagcgca
     3601 ggcgctgcgc gtgaacaatg gttttcaggt cgaaaagcgc gcgctcaaat acctcattga
     3661 ttataacgta gtcgaagtgc ttgacctgcg ccagctcctc gcgcgcattg gccaggcgca
     3721 gctcgatcac ctccggcgtg tcctcgccgc gccgctgcaa ccgtgcacgc agctcttccc
     3781 agctgggcgg cagcacgaac accaggatgg cgttgggaaa ctggcgctgt atctgcagcg
     3841 cgccctgcca gtcgatttcc agcaccacgt cgccaccggc ggcgatgcgc gcttcgatgg
     3901 cctggcgcga ggtgccgtaa cgcctgccgt ggacatgggc ccactcgagg aagtcgccgg
     3961 cggcgatctt ggcgtcgaac tcggcgtcat cgacgaagta gtactcgcgc ccgtgtttgt
     4021 cctgcccgcg cggggcgcgc gtggtgtgcg atatggaggg ttgcacgccc gcgtcgacct
     4081 ccatcagtgc cttgaccagg ctcgatttgc ccgagccgct gggtgcggca acgacgaata
     4141 gatttccagg gtgatccata tggggtcagc gcaggcagct atgcattcag gagttgggcc
     4201 ggcacgctgg tggggcggat tctactcacg ggcgcgaaac accagcagca ggttgttggc
     4261 tggcagggcg tggcgttcgg ccagcgccag accggcgccc tgcgcctctg tgcgcaccgc
     4321 ctccaggtcg cgcaggcccc aactggggtg gcggcggcgc aactcggcgt cgaaggcgtg
     4381 gttgctgggc gcggtcggca ggtcggcttc gatgaagggg ccgtacacca ccagcgcccc
     4441 gccgggggcc aggtggcgcg cggcgccgcg catcagcgcc gcgcaggtgg gccaggggct
     4501 gatgtgcagc atgttggcgc agaagatcag gtcgaaggca ccgtcggcaa acggtgcgcc
     4561 ttcgctgggc catcgtgagt cgagcacgtc cagccgcacc gggcgccgca cgttgcgtgc
     4621 ctgcagccga tcggcccagc cttgcacggt ggcaaacccg gcatcgctga cgtcgctggg
     4681 ctgccagctc cagccgggca gggcgtgggc aaagtgggcg gcgtgctgac cggtgccgct
     4741 ggcgatttcg agcgcgtggc cgctgggcgc cagcagcctg cgcagcacgc cccagatcgg
     4801 cccgcggttg cgctcggcgg cggtgctgca gggcagatcg ggtgtcggtg agtgcatggt
     4861 gatttcgggc ttcagcggtt catggaactg cgcaggtagc tatcataaat gtagcgattt
     4921 cggggcgcgc gccgccggtc tgcagtgcag ccggcaggtc ctgggtcagg ccccgccgcc
     4981 gcgccattca tgcgccggca ctgtcctgcc gcacctgcat ggcgatgtcc agcgcgcgca
     5041 ggcgcagcgc cggttcgtag atgtcgcagg tgaacatcat ctcgtcgcaa tcggtggcgt
     5101 cggccagcgc ttgcaggccc tggcgcacgg tgtccggcgc gccgatcacg gcgcaggcca
     5161 ggaagtcggc gatgccggcg cgcgccggcg cgtccaggcg ctccagaaag ccttcctgcg
     5221 gcggctgcag cagcccgcgc gcgccgcgca gaatgcccag cacgcgggca aagatgctgc
     5281 tggccaggaa catggcctcg gcgtcggtcg gcgccgccac cagcggcacg ccgatcatca
     5341 cgtagggttt gtcgagcacc gccgacggcc gaaagccgcg ccgatacacc tcgatcgcct
     5401 gcagcagcag gcccggcgcg aagtgcgagg caaaggcata gggcaggccg cgctcggccg
     5461 ccagctgagc cgaaaacagg ctggagccca gcagccagat cggcacccgg gtgccggcgc
     5521 cggggttggc gatcagcttc tgcccgggct gcgggcggtc cagcaggcgc tgcaactcgg
     5581 ccacgtcgcg cggaaagtcg tcggccgtct cgatgcggtc gcgccgcagc gcgcgcatgg
     5641 tcagcgggtc ggtgccgggg gcgcgcccca ggcccaggtc gatgcggccg ggaaacagct
     5701 cggccagggt gccgaaagct tcggccacca ccagcggcgc gtggttgggc agcatcacac
     5761 cgccggagcc tacgcggatg cgctcggtgc cgccggcaat gtgcccgacc agcacggcgg
     5821 tggccgaact ggcgatgccc gccatgttgt ggtgttcggc cagccagtag cgggtgaagc
     5881 ccaggcgctc ggcgtgctgc gcggtctgca gcgccaccgc cagcgcgtcg gcgacgctgc
     5941 ggccttcgcg caccggcacc aggtcgagca tggacaaggc gatgtcggac agatttcggt
     6001 tcatgggtcg attgtgtcgc gtcgcacgat cgcaaccggc catccatatc cagtggcctt
     6061 gccggtcgct ggagggctct ctatcatcgc gcggatgggg ttcgcgagga aggg
//
LOCUS       DAOQTZ010000139         6096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_14307_length_6096_cov_14.275782, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000139 DAOQTZ010000000
VERSION     DAOQTZ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14307_length_6096_cov_14.275782"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..3402
                     /locus_tag="PKN51_12365"
     CDS             193..3402
                     /locus_tag="PKN51_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNT85935.1"
                     /translation="MGTDDARRPLSLAKLTAPRPRHAWPRRRLFERLDAARRGGTVIW
                     IAAPAGSGKTTLVASYLQARRLRAVWYRIDAADADPASFFHDLAQAAGSRRRADALPP
                     FTAEYLAGLEVFARNWFRRFFARLQGTPLLVLDDHHLIDADSPVHLALREGLAEVPPG
                     MAVIVTSRAEPPALLTRLRLQEGFTLLDGEALRLSADECVGVARQRLAGAAPADEVLQ
                     QLHQRTQGWVAGLVLMLERGAAATLDEATPAAEPLVFDYFAGELLAQADARVQRFLLL
                     TALLPQVSARAAAALTAQQDAAALLAELERRNFFVARHAEPGRGEAWQYHPLFREFLL
                     ARGRRELTDDELAGLRSQAAALLAAEGDAPAAVALLREAGDWAQLVPLVLEQAPALLR
                     SGRFQTLGEWILAIPQAQRAVSPWLDYFLGLCRLPYDPLQARTLLSSAFTGFRAAGHA
                     AGEYLSWAGVVDTFLYAWSDFGPADPWIEEFDALRQRHPQFPSREAELRAVAAIFALL
                     MYRRPQHPELPQWTHRLDALVAQDRGEPAWRMTAAVHLVLYYEWWVGKLARANELVRR
                     LEPFASARDVGPFVRLAWEGILAISHWMNADNEAALAAVRRGLALADETGAHVWDFML
                     VAQGAVAAGTSGDAGQARDFLERLGERAPGYRPLDSVQYHFFSFQQAMRRDDRAAMLA
                     HGRAGLASALAAGVVWAEVYTRPALAQALFLAGDEAGARREIERATQVAAAVGCSNAF
                     YYIHELEAQFAEARGAHDAQRAALARMFQVMREQGFCNSAWWRDEPMARWCRIALEQG
                     IDVEFVRALIRRRGLRPDGPAARLEHWPWPLRLRTLGRFTIELDGQPLAFSGKVQKRP
                     LALLKALVAFGAQGVPEEQLAEALWPDAEGDDAHHAFTTTLQRLRRLLGQHEALLLQE
                     GRLSLNPQLCWVDAQAFESIDPDARDPAVGPDRERALRLYRGAFLAQEDAAWAIAPRE
                     RLRAHFVRLGGVDVGQHLAAADWEGAVACLERLLHADPTVEPFYQQLMRAQHQLGRSA
                     QVLATYRRCREVLGATLQARPAASTEALLKELDAGGRNV"
     gene            complement(3333..3938)
                     /locus_tag="PKN51_12370"
     CDS             complement(3333..3938)
                     /locus_tag="PKN51_12370"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HNT85936.1"
                     /translation="MNHRLLLAAHLVGAAALGAAIVALWWNDRRIARAGDCGTLAGAG
                     RRAQAIVQRLLLPGVLLLAASGVWLLARYYGWSFVRLPWLAAMAALFVFQSVWANTVT
                     RHHGQRLGRLLAGRCATDRLTPELERARRAPLARFGQQLEPLLFALIVAFGLARPMDW
                     VAIAAGVAAAGLLAAALTAYTLRPPASSSFSSASVEAAGRA"
     gene            complement(3935..4393)
                     /locus_tag="PKN51_12375"
     CDS             complement(3935..4393)
                     /locus_tag="PKN51_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85937.1"
                     /translation="MKPLALSLLLTSAALLPLHVHADTLAPAPETPAAAFQRLFAHTP
                     GPVQPPAGAAPDALTRAFNEALWSTPDQAPTIHQAAFDRAGLPPTAQAPESPAVAFQR
                     MLGHTPGPVQPPVGGAPDVLTRVFNDALWSTPATAAAYASARQHTLERAQ"
     gene            complement(4666..>6096)
                     /gene="dacB"
                     /locus_tag="PKN51_12380"
     CDS             complement(4666..>6096)
                     /gene="dacB"
                     /locus_tag="PKN51_12380"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNT85938.1"
                     /translation="PDIAQALAKAGVPTSAVAMVVAPLPPPPGSVTRQPEPVNPSGEA
                     NPAPAAASLALPPPRLAWQPERPMNPASVMKLVTTYAGLDLLGAGHFWKTRVYTLGTL
                     REGTLLGNLLIQGGGDPKLVVERLQELLRAIQDQGVRRIEGDILLDNSVFRLPPHNAA
                     AFDDDPLRPYNVGPDGLLLNFKALVLRFIPEPAQRRVRIESQPPMAGVGVPAELPVTS
                     GACGDWRTRLALDFSDPEQLRLGGRYPLACGEQEWAIAYPQPERYAPRVIEAMWRAAG
                     GALSGQVRWAEQRPVGARVLVTGLSLPLASVIADINKYSNNVMAQQLFLTLSAGDGRA
                     SFAESRNLLRRWWRERFGLRTTPLVDNGSGLSRDERATAAALAALLQQAASHPQGAVF
                     EQSLSLAGVDGTTRRLAQRRPGSAAIGNARLKTGSLRDVAAIAGYAYGQSGQNYAVVG
                     LINHPNAGAARAALDRLVEWAVQDAP"
BASE COUNT          750 a         2220 c         2316 g          810 t
ORIGIN      
        1 cctggcacgc cgatcacggc acgggcgggc gcgtccaact gccgtttcta ggctgaatcg
       61 ccctcggtcc ggggcgccgc cggcgctcga cgccggggcc cggcgtggct accatgtgtg
      121 gatcccgcgt ccggccatcg ggccggcgga cgcggcggtt cgacccggcc cggtgaagtg
      181 gatggggcga tcatgggcac tgacgacgcg cggcggccgc tcagcctggc caagctgacg
      241 gcacccaggc cccggcacgc ctggccacgg cggcgcctgt tcgagcgcct ggacgccgcg
      301 cggcgcggcg gcacggtgat ctggatcgcc gccccggccg gctcgggcaa gaccacgctg
      361 gtggcgagct acctgcaggc gcgccgcctg cgcgccgtct ggtaccgcat cgatgcggcc
      421 gacgccgacc cggcctcctt cttccacgat ctggcgcagg ccgccggatc gcgccgccgc
      481 gccgacgcgc tgccgccctt caccgccgaa tacctggccg ggctggaagt gttcgcgcgc
      541 aactggtttc gccgcttctt tgcgcgcctg cagggcacgc cgctgctggt gctggacgac
      601 catcacctga tcgacgccga ttcgccggtg cacctggcgc tgcgcgaggg cctggccgag
      661 gtgccgccgg gcatggcggt gatcgtcacc agccgcgccg agccgcccgc gctgctgacc
      721 cggctgcgcc tgcaggaagg cttcaccctg ctggatggcg aggcgctgcg cctgagcgcc
      781 gacgaatgcg tgggcgtggc gcgccagcgg ctggcgggcg ccgcgccggc cgacgaggtg
      841 ctgcagcagc tgcaccagcg cacccagggc tgggtggccg ggctggtgct gatgctcgag
      901 cgcggcgccg ccgccaccct ggacgaggcg acgccggccg ccgagccgct ggtgttcgac
      961 tacttcgccg gcgagctgct ggcgcaggcc gatgcgcgcg tgcagcgctt tctgttgctc
     1021 accgccctgc tgccgcaggt cagcgcccgc gcggcggcgg cgctgacggc gcagcaggac
     1081 gcggcggcgc tgctggccga gctcgaacgg cgcaacttct tcgtcgcccg ccatgccgag
     1141 ccgggccgcg gcgaggcctg gcagtaccac ccgctgtttc gcgaattcct gctggcgcgc
     1201 ggccgccgcg agctgaccga cgacgagctg gcgggcctgc gcagccaggc tgcggccctg
     1261 ctggcggccg agggcgacgc gccggcggcg gtggcgctgc tgcgcgaggc cggcgactgg
     1321 gcgcagctgg tgccactggt gctggaacag gcgccggcgc tgctgcgcag cgggcgcttt
     1381 cagacgctgg gcgaatggat cctggccatc ccgcaggcgc agcgtgcggt ttcgccctgg
     1441 ctcgactact tcctcggcct gtgccgcctg ccctacgacc cgctgcaggc gcgcacgctg
     1501 ctgtcaagcg ccttcaccgg ctttcgcgcc gccggtcacg ccgccggcga atacctgtcc
     1561 tgggccggcg tggtcgacac ctttctctac gcctggagcg acttcggccc ggccgatccg
     1621 tggatcgagg agttcgacgc cctgcgccag cgccacccgc agtttccgag ccgtgaagcc
     1681 gagctgcgcg cggtggcggc catctttgcg ctgctgatgt accggcgccc gcagcacccc
     1741 gagctgccgc agtggacgca ccggctggac gcgctggtgg cgcaggaccg gggcgagccg
     1801 gcgtggcgca tgacggcggc ggtgcacctg gtgctgtact acgagtggtg ggtcggcaag
     1861 ctggcgcgtg ccaacgaact ggtgcggcgc ctcgagccct ttgccagcgc gcgcgacgtc
     1921 ggccccttcg tgcgcctggc ctgggagggc atcctggcga tctcgcactg gatgaacgcc
     1981 gacaacgagg ccgccctcgc cgccgtgcgg cgcggcctgg cgctggccga cgagaccggc
     2041 gcccacgtgt gggacttcat gctggtggcg cagggcgcgg tggccgccgg cacctcgggc
     2101 gacgccgggc aggcgcgcga ctttctggag cgcctgggcg agcgcgcccc cggctaccgc
     2161 ccgctcgaca gcgtgcagta ccacttcttt tcgttccagc aggcgatgcg gcgcgacgac
     2221 cgcgccgcca tgctggcgca cggccgggcc ggcctggcca gcgcgctggc cgcgggcgtg
     2281 gtctgggccg aggtctacac ccgcccggcg ctggcgcagg cgttgtttct ggccggcgac
     2341 gaagccgggg cgcggcgcga gatcgagcgc gccacccagg tggccgcggc cgtcggctgc
     2401 agcaacgcct tctattacat ccacgagctc gaagcccagt tcgccgaggc gcgcggcgcg
     2461 cacgacgccc agcgcgcggc gctggcgcgc atgttccagg tcatgcgcga gcagggcttt
     2521 tgcaactcgg cctggtggcg cgacgagccg atggcgcgct ggtgccgcat cgcgctggag
     2581 caggggatcg acgtcgagtt cgtgcgcgcg ctgatccggc gccgcggcct gcggcccgat
     2641 ggtccggccg cgcgcctgga acactggccg tggccgctgc gcctgcgcac gctgggccgg
     2701 ttcacgatcg agctcgacgg ccagccgctg gccttcagcg gcaaggtgca gaaacgcccg
     2761 ctggcgctgc tgaaggcgct ggtcgccttc ggtgcccagg gcgtgcccga agagcagctg
     2821 gccgaggcgc tgtggcccga cgccgaaggc gacgatgcgc accacgcctt caccaccacc
     2881 ttgcagcggc tgcgccgcct gctcggccag cacgaagcgc tgctgctgca ggaagggcgc
     2941 ctgagcctga acccacagct gtgctgggtc gatgcgcagg cgtttgagtc gatcgacccc
     3001 gatgcgcgcg acccggcggt cggccccgat cgcgagcgcg ccctgcgcct gtaccgcggc
     3061 gcctttctgg cgcaggaaga cgcggcgtgg gcgatcgcgc cgcgcgagcg cctgcgcgcg
     3121 cacttcgtgc gcctgggcgg cgtcgatgtg ggccagcacc tcgcagccgc cgactgggag
     3181 ggcgccgtcg cctgtctgga acgcctgctg cacgccgacc ccacggtcga gccgttctac
     3241 cagcagctga tgcgcgcgca gcaccagctc gggcgcagcg cgcaggtgct ggccacctac
     3301 cggcgctgcc gcgaggtgct gggcgccacg cttcaggcgc ggcccgcggc ctcgaccgag
     3361 gcgctgctga aggaactcga cgcgggtggc cgcaacgtgt aggcggtcag cgccgccgca
     3421 agcagccccg ccgcggcgac gccggcggcg atcgccaccc agtccatcgg ccgcgccagg
     3481 ccgaaggcga cgatgagcgc gaacagcagc ggttcgagct gctggccaaa gcgcgccagc
     3541 ggtgcgcgcc gggcccgctc cagctcgggc gtgagccggt cggtggcgca gcggccggcc
     3601 agcaggcggc ccaggcgctg cccgtgatgg cgcgtgacgg tgttggccca gaccgactgg
     3661 aacacgaaca gcgccgccat ggcggccagc cagggcaggc gcacgaagct ccagccgtag
     3721 tagcgcgcca gcagccagac gcccgaggcc gccagcagca gcacgccggg cagcagcagg
     3781 cgctggacga tcgcctgggc gcgccggccg gcgccggcca gcgtgccgca gtcgccggcg
     3841 cgcgcgatgc gccggtcgtt ccaccacagc gcgacgatcg ccgcgcccag ggcggccgca
     3901 cccacaaggt gcgccgccag cagcagccgg tggttcattg cgcgcgctcc agcgtgtgct
     3961 gccgggccga ggcgtaggcg gccgccgtgg ccggggtgct ccacagcgcg tcgttgaaga
     4021 cgcgcgtcag cacgtcgggc gcgccgccga cgggcggctg caccggcccc ggggtgtggc
     4081 cgagcatgcg ctggaacgcc accgccggtg actcgggcgc ctgcgccgtc ggcggcagac
     4141 cggcgcgatc gaacgccgcc tggtggatcg tgggcgcctg atcgggggtg ctccacagcg
     4201 cctcgttgaa ggcgcgtgtc agcgcgtcgg gcgcggcacc ggcgggcggc tgcaccggcc
     4261 ccggggtgtg ggcgaacagg cgttggaacg cggccgccgg cgtttcgggc gcgggcgcca
     4321 gggtgtcggc gtggacgtgc agcggcagca gggcggcgct ggtcagcagc agggacagag
     4381 cgagcggttt catggcgtgg tcctcaatca agatggtcgg gggtcttcgc ttggcccggg
     4441 ccatcggcac cgggcgtcgc ggaggccatg gttggcgccg ctcacccacg gatcgctaac
     4501 ggatccggcc gtgatcgagg atttacgttc tgaagcaaaa agggccgccg cgggcgccgt
     4561 ggggcgttgc atcagcccaa aatgctatgt attagatagc ttgcggcgct tgcccgacgg
     4621 gccctggagg ccgaaatcgc tctgaatggc tgacgccggg cgcgctcagg gcgcgtcctg
     4681 caccgcccac tccaccagcc ggtccagcgc cgcgcgggcg gcgccggcgt tggggtggtt
     4741 gatgaggccg accacggcgt agttctggcc cgactggcca taggcgtagc cggcgatggc
     4801 ggccacgtcg cgcaggctgc cggttttcag gcgcgcgttg ccgatcgccg ccgaccccgg
     4861 gcggcgctgg gccagccggc gcgtggtgcc gtccaccccc gccagcgaca gcgactgctc
     4921 gaacaccgcc ccctgcggat ggctggccgc ctgctgcagc agcgccgcca gcgcggccgc
     4981 ggtggcgcgt tcgtcgcgcg acagtccgga gccgttgtcc accagcggcg tggtgcgcag
     5041 cccgaagcgc tcgcgccacc agcgccgcag caggttgcgc gattcggcga agctggcgcg
     5101 accgtcgccc gccgacagtg tcaggaacag ctgctgcgcc atcacgttgt tggagtactt
     5161 gttgatgtcg gcaatcacgc tggccagcgg cagcgacagg ccggtcacca gcacgcgtgc
     5221 gccgaccggg cgctgctcgg cccagcgcac ctgcccgctc agggcgccgc cggcggcgcg
     5281 ccacatggcc tcgatcacgc gcggcgcgta acgctcgggc tgcgggtagg cgatggccca
     5341 ttcctgctcg ccgcaggcca gcggatagcg cccgcccagc cgcagctgct cggggtcgct
     5401 gaagtcgagc gccagccgcg tgcgccagtc gccgcaggcg ccgctggtga ccggcagctc
     5461 ggccggtacg ccgacgccgg ccatgggcgg ctggctttcg atgcgcacgc ggcgctgcgc
     5521 cggctcgggg atgaagcgca gcaccagcgc cttgaagttc agcagcagcc cgtccggccc
     5581 cacgttgtag gggcgcaggg ggtcgtcgtc gaaggcggcg gcgttgtgcg gcggcaggcg
     5641 gaacacgctg ttgtccagca ggatgtcgcc ctcgatgcgg cgcacgccct ggtcctggat
     5701 ggcccgcagc agctcctgca ggcgctcgac caccagcttg gggtcgccgc cgccctggat
     5761 cagcaggttg ccgagcagcg tgccctcgcg cagcgtgccc agggtgtaga cccgggtctt
     5821 ccagaagtgg cccgcgccca gcaggtccag cccggcgtaa gtggtcacca acttcatcac
     5881 cgacgcaggg ttcatcggcc gctcgggctg ccaggccagg cgcggcggtg gcagcgccag
     5941 gctggcagcg gccggtgccg ggttggcttc gccgctgggg ttgaccggct ccggctggcg
     6001 cgtcacgctg cccggtggcg gcggcagcgg cgccaccacc atggccacgg cgctggtcgg
     6061 cacgccggcc ttggccagcg cctgggcgat gtcggg
//
LOCUS       DAOQTZ010000140         6085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_14335_length_6085_cov_12.565506, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000140 DAOQTZ010000000
VERSION     DAOQTZ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14335_length_6085_cov_12.565506"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PKN51_12385"
     CDS             <1..1268
                     /locus_tag="PKN51_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNT85939.1"
                     /translation="RAARLLWCRIMKGFGAKNPKSLMLRTHCQTSGWSLTEQDPYNNV
                     VRTAIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQLIIQEETHITSVVDPW
                     AGSYMMESLTQQMADAAWAIIEEVDAMGGMTKAVDSGWAKLKIEAAAAEKQARIDSGK
                     DVIVGVNKYKLAREDAVDILEVDNVKVREAQIARLKDIKQKRDPAQVQQALAAITTIA
                     QNGQGNLLAAAVDAIRARATVGEVSDAMEQVFGRHRADTQKVTGVYAAAYEGAEGWDK
                     LKQEIAGFAEAEGRRPRVMIAKLGQDGHDRGAKVVATAFADLGFDVDIGSLFQTPEEC
                     ARQAIENDVHAVGVSTLAAGHKTLVPAIINELKKQGADDIVVFVGGVVPRQDYDFLYD
                     AGVKGIYGPGTPIPASAKDVLEQIRQARP"
     gene            1321..2340
                     /gene="meaB"
                     /locus_tag="PKN51_12390"
     CDS             1321..2340
                     /gene="meaB"
                     /locus_tag="PKN51_12390"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNT85940.1"
                     /translation="MSPAVSAQAITQSRGPAQRRAIAKAITLLESTRADHRAQGDELL
                     TALLPHTGGALRLGISGVPGVGKSTFIEALGVYLVERGHRVAVLAIDPSSTVSGGSIL
                     GDKTRMERLSALEGAYIRPSPSSGTLGGVAEKTREAMLVCEAAGYDVVLVETVGVGQS
                     ETAVHGMTDMFCVLQLPNAGDDLQAIKKGVMELADLVVINKADLDPAAATRARAQIQS
                     ALRVFGQQGNPQHALQDASVWHPEVIQLSGLKGEGLDAFWDCVSRFRALQSAGGRLHE
                     RRRQQSLAWMWERIDAGLKAAFRAHPEVRQALPAMTQDVAQGRLPPSTAARRLLALVA
                     PESVA"
     gene            2337..3167
                     /locus_tag="PKN51_12395"
     CDS             2337..3167
                     /locus_tag="PKN51_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85941.1"
                     /translation="MTAMALSPQDLQRIAQSPGWRGGRVEVCDTSQGKVIVKGWRPAH
                     HPARYGLLNRLARWLGLPFLRAVPLAGGAQAQHTEVARLRALRRAGARVPEVLHVGSD
                     HFVMQWLGRAHLGDVLQSGHPGAAALWREAGDELVRLHRAGQYLSQGFARNMIVTDEA
                     GDTPRLAGLIDFEDDPLQVMGLADAQVRDWLAFLHSTLWILPLAPSEVDTCIDAWLDA
                     ESPAVRAGFAQACRRLAWLRVLPRQRRFGRDTVALQAAAAAAHRHARRHPISLDNRSN
                     "
     gene            3177..4709
                     /locus_tag="PKN51_12400"
     CDS             3177..4709
                     /locus_tag="PKN51_12400"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNT85942.1"
                     /translation="MKPILEQLEKKRAAARLGGGHKRIDAQHAKGKLTARERIEVLLD
                     AGSFEEWDMFVEHRCADFGMDATHIPGDGVVTGYGTINGRLVFVFSQDFTVFGGALSE
                     AHAEKICKVMDQAMKVGAPVIGLNDSGGARIQEGVASLGGYAEVFQRNVMASGVIPQI
                     SMIMGPCAGGAVYSPAMTDFIFMVKDTSYMFVTGPEVVKTVTHESVTSEQLGGALTHT
                     TRSGVADLAFENDVEALLMLRRFFNYVPASNREKAPYRPTQDPADRADLSLDTLVPDN
                     PNLPYDIKEAIVKTVDDGEFFELQPDYAANIVIGFGRMDGHVVGIVANQPLVLAGCLD
                     IKSSIKAARFVRFCDAFNIPVVTFVDVPGFMPGTAQEYGGIIKHGAKLLYAYAECTVP
                     KITVITRKAYGGAYDVMSSKHLRGDVNLAWPNAEIAVMGAKGAVEIIFREEKKDPAKL
                     AAREAEYKARFANPFVAGARGFIDDVILPHETRQRICRSLAMLKDKKLDNPWRKHGNI
                     PL"
     gene            4730..5023
                     /locus_tag="PKN51_12405"
     CDS             4730..5023
                     /locus_tag="PKN51_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNT85943.1"
                     /translation="MPKAANAQLARQLTDIPNIGPSIASDLRLLGIRSPADVRAMDPL
                     AAYDALRTPMGHRHDPCVLDTFLAAHDFMHGGQARPWWHFTARRKALLASLKR"
     gene            5167..>6085
                     /locus_tag="PKN51_12410"
     CDS             5167..>6085
                     /locus_tag="PKN51_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNT85944.1"
                     /translation="MFTKILIANRGEIACRVIATARKMGIATVAVYSDADAQARFVQM
                     ADEAVRLGPPPSRESYLLGDKIIEAAKATGAQAIHPGYGFLSENEAFARKVEEQGLVF
                     IGPKHHSIAAMGDKIASKKLADQAKVNTIPGYNDPIETPEQAVEIAKGIGYPVMIKAS
                     AGGGGKGLRVAFNDKEAHEGFSSCRNEARNSFGDDRVFIEKFVEQPRHIEIQVLGDAH
                     GNVVYLNERECSIQRRHQKVIEEAPSPFISEATRRAMGEQAVALAKAVKYQSAGTVEF
                     VVGKNQDFYFLEMNTRLQVEHPVTECITGL"
BASE COUNT         1094 a         2102 c         2057 g          832 t
ORIGIN      
        1 tgcgcgccgc gcgcctgctg tggtgccgca tcatgaaggg cttcggcgcc aagaatccca
       61 agagcctgat gctgcgcacc cattgccaga ccagcggctg gagtctgacc gaacaggacc
      121 cctacaacaa cgtggtgcgc accgccatcg aggccatggc ggcggtgttc ggcggcacgc
      181 agagtctgca caccaacgca ctcgacgagg cgatcgcact gccgaccgag ttttcggccc
      241 gcatcgcgcg caacacccag ctcatcatcc aggaagagac ccacatcacc agcgtggtcg
      301 atccctgggc cggcagctac atgatggagt cgttgaccca gcagatggcg gacgccgcct
      361 gggccatcat cgaagaggtc gacgccatgg gcggcatgac caaggcggtg gacagcggct
      421 gggccaagct caagatcgaa gctgccgcgg ccgaaaaaca ggcccgcatc gactccggca
      481 aggacgtcat cgttggcgtc aacaaataca agctggccag ggaagacgcg gtcgacatcc
      541 tggaggtgga caacgtcaag gtgcgtgaag cgcagatcgc ccgcctcaag gatatcaagc
      601 aaaaacgcga cccagcgcag gtacagcaag cgctggcagc tattacaacg atagcacaaa
      661 acgggcaggg caacctgctg gcggccgccg tcgatgccat tcgtgcccgc gccaccgtgg
      721 gcgaagtgag cgatgccatg gaacaggtct tcgggcgcca ccgcgccgac acgcagaaag
      781 tcaccggcgt ctacgccgcc gcctacgaag gcgccgaggg ctgggacaag ctcaagcagg
      841 agatcgccgg cttcgccgag gccgagggcc gccgcccgcg cgtgatgatc gccaagctgg
      901 gccaggacgg ccacgaccgc ggcgccaagg tggtggccac cgcctttgcc gacctgggct
      961 tcgacgtcga catcggcagc ctgttccaga cccccgagga atgtgcgcgc caggcgatcg
     1021 aaaacgacgt ccacgcggtc ggcgtcagca cgctcgcggc cggccacaag accctggtgc
     1081 cggccatcat caacgaactg aagaagcagg gtgcggacga catcgtggtc ttcgtcggcg
     1141 gcgtggtgcc gcgccaggac tacgacttcc tgtacgacgc cggcgtcaag ggcatctacg
     1201 gccccggcac ccccatcccg gccagcgcca aggatgtgct ggagcagatt cgccaggcgc
     1261 gtccatgacg gctcccaccg cttgggcttc gaccgcctca gcacgaacga gagggcggat
     1321 gtgagccccg ccgtatccgc ccaggccatc acgcagagcc gaggcccggc ccagcgccgc
     1381 gccattgcca aggccatcac cctgctcgaa tcgacccgtg ccgaccaccg ggcgcagggc
     1441 gacgagctgc tgacggcgct gctgccgcac accggcggtg cgctgcgcct gggcatcagc
     1501 ggcgtgccgg gggtgggcaa gagcaccttc atcgaagcgc tcggggtgta tctggtggag
     1561 cgcggccacc gggtggcggt gctggcgatc gacccttcgt ccaccgtctc gggtggctcg
     1621 atcctgggcg acaagacgcg catggaacgc ctgtcggcgc tggaaggcgc ctacatccgc
     1681 cccagcccca gcagcggcac gctgggcggc gtggccgaga aaacgcgcga ggccatgctg
     1741 gtgtgcgagg cggccggcta cgacgtggtg ctggtcgaaa ccgtgggcgt cggccagagc
     1801 gaaaccgccg tgcacggcat gaccgacatg ttctgcgtgc tgcagctgcc caacgcgggc
     1861 gacgacttgc aggcgatcaa gaaaggcgtg atggagctgg ccgacctggt ggtcatcaac
     1921 aaggccgacc tcgacccggc ggcggccacg cgggcgcggg cgcagatcca gtcggcgttg
     1981 cgcgtgttcg gccagcaggg caatccgcag catgcgctgc aggacgccag cgtctggcac
     2041 ccggaggtga tccagctcag cggcctgaag ggcgagggcc tggatgcgtt ctgggactgc
     2101 gtgagccggt tccgcgccct gcaaagcgcc ggcggccggc tgcacgagcg acgccggcag
     2161 cagtcgctgg cctggatgtg ggagcgcatc gatgccggcc tgaaggcggc cttccgcgcc
     2221 caccccgagg tgcgccaggc gctgccggcc atgacgcagg acgtcgccca gggccggctg
     2281 ccgccgtcca ccgcggcgcg ccggctgctg gcgctggtgg cgccggagag cgtcgcgtga
     2341 ccgccatggc gctcagcccg caggacctgc aacgcatcgc gcagtcgccc ggctggcgcg
     2401 gcgggcgggt ggaggtctgc gacacctcgc agggcaaggt gatcgtcaag ggctggcgac
     2461 cggcccatca cccggcccgc tatggcctgc tcaaccgcct ggcgcgctgg ctggggctgc
     2521 cgtttctgcg cgccgtgccg ctggccggcg gcgcccaggc gcagcacacc gaggtggccc
     2581 gcctgcgcgc gctgcgccgc gccggcgcgc gcgtgcccga ggtgctgcac gtgggcagcg
     2641 accatttcgt catgcaatgg ctgggacgcg cccacctggg cgacgtgctg cagtcggggc
     2701 acccgggcgc ggccgcgctg tggcgcgagg ccggcgacga gctggtgcgc ctgcaccggg
     2761 ccggccagta cctcagccag ggctttgcgc gcaacatgat cgtcaccgac gaagccggcg
     2821 acaccccgcg cctggccggg ctgatcgact tcgaggacga cccgctgcag gtcatgggtc
     2881 tggccgacgc ccaggtgcgc gactggctgg ccttcctgca cagcaccttg tggatcttgc
     2941 ccttggcgcc gtccgaagtg gacacctgca tcgacgcctg gctggacgcc gaaagcccgg
     3001 ccgtgcgcgc cggctttgcc caggcctgcc ggcgcctggc ctggctgcgg gtgctgccgc
     3061 gccagcgccg atttggccgc gacacggtgg cgttgcaggc cgccgccgca gcggcgcacc
     3121 gccacgcccg ccgtcacccg atctcccttg ataaccgcag caactgaagg acccccatga
     3181 aaccgatcct ggaacagctt gagaaaaagc gcgccgccgc ccgcctgggc ggcgggcaca
     3241 agcgcatcga cgcccagcac gccaagggca agctgacggc gcgcgagcgc atcgaggtgc
     3301 tgctggacgc cggcagcttc gaggaatggg acatgttcgt cgagcaccgc tgcgccgact
     3361 tcggcatgga cgccacccac atccccggtg acggcgtggt gaccggctac ggcaccatca
     3421 acggccgcct ggtgttcgtc ttcagccagg acttcacggt gttcggcggc gccctgagcg
     3481 aggcgcacgc cgagaagatc tgcaaggtga tggaccaggc catgaaggtc ggcgcgccgg
     3541 tgattggcct gaacgactcg ggcggcgcgc gcattcagga gggcgtggcc agcctgggcg
     3601 gctacgccga ggtgtttcag cgcaacgtga tggccagcgg cgtgatcccg cagatcagca
     3661 tgatcatggg gccgtgcgcg ggtggcgcgg tgtacagccc ggccatgacc gacttcatct
     3721 tcatggtcaa ggacaccagc tacatgttcg tcaccggccc cgaggtggtc aagaccgtga
     3781 cgcacgagag cgtgacgtcg gagcagctgg gcggcgcgct gacccacacc acgcgcagcg
     3841 gcgtcgccga cctggcgttc gagaacgacg tcgaggcgct gctgatgctg cggcgctttt
     3901 tcaactacgt gccggccagc aaccgcgaaa aggcgccgta ccggccgacc caggacccgg
     3961 ccgaccgcgc cgacctgagc ctggacaccc tggtgccgga caacccgaac ctgccctacg
     4021 acatcaagga agccatcgtc aagacggtgg acgacggcga gttcttcgag ctgcaacccg
     4081 actacgccgc caacatcgtg atcggcttcg gccgcatgga cggccacgtg gtgggcatcg
     4141 tcgccaacca gccgctggtg ctggccggct gcctggacat caagagctcg atcaaggcgg
     4201 cgcgcttcgt gcgcttctgc gacgccttca acatcccggt ggtgaccttc gtggacgtac
     4261 ccggcttcat gcccggcacc gcgcaggaat acggcggcat catcaagcac ggcgccaagc
     4321 tgctgtacgc ctacgccgaa tgcaccgtgc ccaagatcac ggtgatcacg cgcaaggcct
     4381 acggcggcgc ctacgacgtg atgagttcca agcacctgcg cggcgacgtc aacctggcct
     4441 ggcccaacgc cgaaatcgcc gtgatgggcg ccaagggcgc ggtggaaatc atcttccgcg
     4501 aggaaaagaa ggaccccgcc aagctcgccg cgcgcgaggc cgagtacaag gcgcgctttg
     4561 ccaacccctt cgtggccggg gcacgcggct tcatcgacga cgtgatcctg ccgcacgaga
     4621 cgcgccagcg catctgccgc agcctggcca tgctgaaaga caagaagctg gacaacccgt
     4681 ggcgcaagca cggcaacatt ccgctctgaa aagcatagct gccagcgcaa tgcccaaggc
     4741 cgccaacgct caactggccc gtcagttgac ggacatcccg aacatcgggc cgtcgatcgc
     4801 gtccgacctg cgcctgctgg gcatccgctc gcccgccgat gtgcgcgcca tggatccgct
     4861 ggcggcctac gatgccctgc gtacccccat ggggcaccgc cacgacccgt gcgtgctgga
     4921 caccttcctg gccgcgcacg acttcatgca tggcggccag gcgcgcccat ggtggcactt
     4981 caccgctcga cgcaaggcct tgctcgcctc cctgaaacgc tgacgagaga taccgacatg
     5041 ttcaaccaga tcctgattgc caaccgcggc gaaaacggcc gcgcggcgga cgtttcgacc
     5101 aatggcttgc cgcagcccgt gcagcgggct tctgcacgcc acaccaccgc ttgaggaacc
     5161 gcaaccatgt ttaccaaaat cctgatcgcc aaccggggcg agattgcgtg cagggtcatc
     5221 gccaccgccc gcaagatggg catcgccacc gtggcggtgt attccgacgc cgacgcgcag
     5281 gcccgcttcg tgcagatggc cgacgaggcc gtgcgtctgg ggccgccgcc ctcgcgcgag
     5341 tcctacctgc tgggcgacaa gatcatcgag gcggccaagg ccaccggggc gcaagccatc
     5401 catccgggct atggcttcct gagcgagaac gaggccttcg cgcgcaaggt cgaggaacag
     5461 ggcctggtgt tcatcgggcc caagcaccac agcatcgcgg ccatgggtga caagatcgcg
     5521 tccaagaagc tggccgacca ggccaaggtc aacaccattc cgggctacaa cgacccgatc
     5581 gagacgcccg agcaggcggt ggaaatcgcc aagggcatcg gctacccggt gatgatcaag
     5641 gccagcgccg gcggcggcgg caagggcctg cgcgtggcct tcaacgacaa ggaggcgcac
     5701 gagggcttca gctcgtgccg caacgaagcg cgcaacagct tcggcgacga ccgcgtgttc
     5761 atcgaaaaat tcgtcgagca gccgcgccac atcgaaatcc aggtgctggg tgacgcgcac
     5821 ggcaacgtgg tctacctcaa cgagcgcgaa tgctcgatcc agcgccgcca ccagaaggtg
     5881 atcgaagagg cgccgtcgcc cttcatctcc gaggcgacgc gccgcgccat gggcgagcag
     5941 gccgtggcgc tggccaaggc cgtcaagtac cagtcggccg gtacggtgga gttcgtggtc
     6001 ggcaagaacc aggactttta tttcctggag atgaacaccc gcctgcaggt ggagcacccg
     6061 gtgacggagt gcatcaccgg cctgg
//
LOCUS       DAOQTZ010000141         5928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_14805_length_5928_cov_15.214711, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000141 DAOQTZ010000000
VERSION     DAOQTZ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14805_length_5928_cov_15.214711"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKN51_12415"
     CDS             <1..724
                     /locus_tag="PKN51_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT85945.1"
                     /translation="FSMVFQAADGPVWVEHLMAKLPGGQIGFLIFVNVMIFLLAFFLD
                     FFELSFIVVPLLAPIADKMGIDLIWFGVLLAVNMQTSFMHPPFGFALFFLRSVAPEKP
                     YVDHVTGKTIEPVTTMQIYKGAIPFLVLQLTMVAILIAFPGLVTSNLDKKVDVDMKAV
                     GEQMFEQLNQDSEAEGLGEGREDGIGTQEPGSSSEESPAIEGLEAPADPSSPEAGQEG
                     NQGQEDDDPVKALERAAGKPSS"
     gene            complement(813..1895)
                     /gene="dctP"
                     /locus_tag="PKN51_12420"
     CDS             complement(813..1895)
                     /gene="dctP"
                     /locus_tag="PKN51_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNT85946.1"
                     /translation="MDRRSLIKRAGIAGVLAAGVAPAVHAQAAVRWRIATSFPKSLET
                     LHGCAVKFSETVKALSGGKFEVSVHAAGELVPAFGIVDALADDSLEMGETASYYYTGK
                     HPAFAFGCAVPFGFTAQQMQAWKRHGNGRKLMDELYDKYNFFGLSALSTGTQMGGWYR
                     KEMKTPADFKGLKMRLGGGVFGESMAKLGVVAQNMPVGEVYQALEKGTLDAVEFVGPY
                     DDEKLGFNKVAKYYYYGGWWEGSAELEFFINKKAFAKLSPENQAIVRAAADVAYADGT
                     SKYMHLNPISLKRLVGSGTQLKRFPKPVVEAGFKAAQAVYQEHSGKDALFKKIYEDMR
                     AFQRDQILWNRISELAFTQDMSTLKI"
     gene            complement(2012..3916)
                     /gene="dxs"
                     /locus_tag="PKN51_12425"
     CDS             complement(2012..3916)
                     /gene="dxs"
                     /locus_tag="PKN51_12425"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNT85947.1"
                     /translation="MSQALLESIHSPADLRRLPRAQLKGLADELRACVLTNVARTGGH
                     LSSNLGTVELTIALHYVFQTPHDRLVWDVGHQTYPHKILTGRRERMPTLRQLGGMSGF
                     PRRDESEYDTFGTAHSSTSISAALGMALAARQKGEDRHAVAIIGDGAMTAGMAFEALN
                     NAGVERDARLLVVLNDNDMSISPPVGALNRYLAQLMSGHFYAAARSVGKTVLGTVPPL
                     FELARRLEQGAKGMVVPATLFEKFGFNYIGPIDGHDLDSLIPTLENIRDLMRDKAGPQ
                     FLHVVTKKGQGYKLAEADPVAYHGPGKFDPAVGLVKPSAPAAPTFTQVFGQWLCDMAA
                     HDRKLVGITPAMREGSGMVEFHKRFPDRYYDVGIAEQHAVTFAAGLACEGLKPVVAIY
                     STFLQRAYDQLIHDVAIQNLPVVFALDRAGLVGADGATHAGNYDIAFLRCVPNVSLAC
                     PADERECRQLLTTAYEQSHPVAVRYPRGAGAGVAPLAQLTGLPFGKGEVRRQGKGVAI
                     LAFGTLLYPALAAAERLDATVVNMRWVKPLDAELLLQVAASHDALVTIEEGAIMGGAG
                     SAVAEALAAAGVVKPLLHLGLPDRFIEHGDPARLLALEGLDATGIEGSVRSHLGSWLM
                     PPQRNLKAVS"
     gene            complement(3919..4815)
                     /locus_tag="PKN51_12430"
     CDS             complement(3919..4815)
                     /locus_tag="PKN51_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT85948.1"
                     /translation="MNARDFPTWSDRLLTRVEQALVDWVPVDAPAGLGEAMRYAVLDG
                     GKRLRPLLVLAAAEAVQGHAAAALRAACAVELIHAYSLVHDDMPCMDNDVLRRGKPTV
                     HVRFGQAQALLAGDALQALAFELLTPEGDEVPASMQAALCGLLARAAGATGMAGGQAI
                     DLDSVGRVLTEEQLQAMHRLKTGALLQAAVHMGASCATPALPAHTRAALDQYGAALGL
                     AFQVVDDILDVTADSAQLGKTAGKDAAADKPTYVSLLGLTQAQALAAQLHTQAHQALD
                     ASGLTALAPLRWLADRVVKREN"
     gene            complement(4812..5051)
                     /gene="xseB"
                     /locus_tag="PKN51_12435"
     CDS             complement(4812..5051)
                     /gene="xseB"
                     /locus_tag="PKN51_12435"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNT85949.1"
                     /translation="MSRKAASAPAQPASYGEAVQQLEQLIAGLESGQLPLEDLLSHYQ
                     RGTELLRYCRDRLQAVEDQVRVLDERGDVKPWMPE"
     gene            5292..>5928
                     /locus_tag="PKN51_12440"
     CDS             5292..>5928
                     /locus_tag="PKN51_12440"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNT85950.1"
                     /translation="MSDLSLQLQQAASQLPVHSYFDPALYEREKKLLFESGPRYVGHA
                     LAVPEAHDYFVLPQEAGGRALLRDAQGQIQLVSNCCRHRQAIMLGGRGNLLTEGKGHA
                     GGNIVCPIHRWTYSSRGELLGAPHFERDPCLNLSNYPLRQWNGLLFEDNGRDIAADLA
                     GMTTAKDLSFDGYVLDHVELHECNYNWKTFIEVYLEDYHVGPFHPGLGNFVT"
BASE COUNT          968 a         2024 c         1962 g          974 t
ORIGIN      
        1 cttcagcatg gtgttccagg cggccgacgg gccggtgtgg gtcgagcacc tgatggccaa
       61 gctgcccgga gggcagatcg gctttctgat cttcgtcaac gtgatgatct tcctgttggc
      121 gttcttcctg gacttcttcg agctgtcctt catcgtcgtc ccgctgctcg cgccgattgc
      181 cgacaagatg ggcatcgacc tgatctggtt cggggtgctg ctggcggtga acatgcagac
      241 ctccttcatg cacccgccgt ttggttttgc gctgttcttc ctgcgctcgg tcgcacccga
      301 gaagccctat gtggaccatg tgacgggcaa gaccatcgag cccgtgacca ccatgcagat
      361 ctacaagggc gccatcccct tcctggtgct gcagctgacc atggtggcca tcctgatcgc
      421 cttccccggc ctggtgacca gcaacctgga caagaaggtc gacgtggaca tgaaggccgt
      481 gggcgagcag atgttcgagc agctcaacca ggacagcgag gccgaaggat tgggcgaggg
      541 ccgggaagac ggcatcggta cccaggagcc gggcagcagc agcgaagaat cacccgccat
      601 cgaaggcctg gaagctcccg ccgacccgtc ctcaccagag gcaggccagg agggcaacca
      661 gggccaagag gacgacgacc ccgtgaaggc tctggagcgc gcggccggca agccgtcatc
      721 ctgagcggca tgaaagaacc gcagcccttg acaggctgcg gttttttttg tgccccccgg
      781 caattacacc ggggtgcgcc agaccctgac actcagatct tcagcgtgct catgtcctgg
      841 gtgaacgcca gctcggagat gcggttccag aggatctggt cgcgctggaa ggcgcgcatg
      901 tcttcgtaga tcttcttgaa cagggcgtcc ttgccgctgt gctcctgata gaccgcctgc
      961 gcggccttga agccggcttc caccacgggt ttgggaaagc gcttgagctg cgtgcccgag
     1021 cccaccagac gcttgagcga gatggggttg aggtgcatgt acttggaggt gccatcggca
     1081 taagccacgt cggcagcggc gcgaacgatg gcctggttct cgggcgagag cttggcaaac
     1141 gccttcttgt tgatgaagaa ctccagctcc gcgctgcctt cccaccagcc gccgtagtag
     1201 tagtacttgg cgaccttgtt gaaacccagc ttctcgtcgt cgtaggggcc gacgaactcc
     1261 acggcatcga gcgtgccctt ttccagggcc tggtacacct cgcccaccgg catgttctgc
     1321 gccaccacgc ccagcttggc catggactcg ccgaacacgc cgccacccag gcgcatcttc
     1381 agacccttga agtcggccgg ggtcttcatt tccttgcggt accagccgcc catctgggtg
     1441 ccggtgctga gggcgctcag gccgaagaag ttgtacttgt cgtacaactc gtccatcagc
     1501 ttgcgtccgt taccgtggcg cttccaggcc tgcatctgct gggcggtgaa accaaacggc
     1561 acggcgcagc cgaaggcaaa tgccgggtgc ttgccggtgt agtagtacga ggccgtctcg
     1621 cccatttcca gcgaatcgtc ggccagggcg tcgacgatgc caaaggccgg caccagctcg
     1681 cccgcagcat gcacggagac ctcgaacttg ccgcccgaca gggccttgac ggtttcggag
     1741 aacttcacgg cgcagccatg cagggtctcc agcgacttcg gaaagctggt ggctatgcgc
     1801 caacgcacgg ccgcctgcgc atgcaccgcc ggcgccacgc cggcagccag caccccggcg
     1861 atgccggcac gcttgatcag ggaacgacga tccatgttgc gtactcctca aaaaaaagaa
     1921 caaccggtcg agtgtagcgg cttgtaaacc gcgccagcca ttagggcatt cacttgatcg
     1981 gatcgccggg cgccgtggcg cgcctgaatg gtcagctgac cgctttcagg ttgcgctgag
     2041 gcggcatcag ccacgagccc aagtgcgagc gcaccgaacc ctcgatgcca gtggcatcca
     2101 ggccctcgag cgccagcagt cgcgccggat cgccgtgctc gatgaagcgg tcaggcaggc
     2161 ccaggtgcag cagcggcttg accaccccgg ccgcggccag cgcctcggcc acggcgctgc
     2221 cggcgccacc catgatggcg ccctcctcga tcgtcaccag cgcgtcgtgg ctggctgcca
     2281 cctgcagcag cagctcggcg tccagcggct tgacccagcg catgttgacc accgtggcgt
     2341 ccagccgttc ggcggcggca agcgcggggt acagcagcgt gccgaacgcc aggatggcca
     2401 cccccttgcc ctggcgccgg acttcgccct tgccgaacgg cagcccggtc agctgagcca
     2461 gcggtgccac gccggctccg gcaccacgcg ggtagcgcac cgccaccgga tggctctgct
     2521 cgtaggccgt ggtcagcaac tgacggcact cgcgttcgtc ggccgggcag gccaggctga
     2581 cgttgggcac gcagcgcagg aaggcgatgt cgtagttgcc ggcatgggtg gcgccatcgg
     2641 caccgaccag gcccgcgcga tccagcgcga acaccaccgg caggttctgg atggcgacgt
     2701 cgtggatcag ctggtcgtag gcgcgctgca ggaaggtgga atagatggcc accaccggct
     2761 tgaggccctc gcaggccaga ccggcggcaa aggtgacggc atgctgctcg gcaatgccga
     2821 cgtcgtagta gcggtccgga aagcgcttgt ggaattcgac catgcccgaa ccttcgcgca
     2881 tggccggggt gatgcccacc agcttgcggt cgtgcgccgc catgtcgcac aaccactgcc
     2941 cgaacacctg ggtgaaggtg ggcgcggccg gggcgctggg cttgaccagg cccaccgccg
     3001 gatcgaactt gcctgggccg tggtaggcca ccggatcggc ctcggccagc ttgtagccct
     3061 ggcccttttt ggtgaccacg tgcaggaact gcggcccggc cttgtcgcgc atcaggtcgc
     3121 ggatgttttc cagcgtcggg atcagcgaat ccagatcgtg gccgtcgatc ggaccgatgt
     3181 agttgaagcc gaacttctcg aacagcgtgg ccggcaccac catgcccttg gcgccctgct
     3241 ccagccgccg ggccagctcg aacagcggcg gcaccgtgcc cagcaccgtc ttgcccacgc
     3301 tcctggcggc ggcatagaag tggccgctca tcagctgcgc caggtagcgg ttgagcgcgc
     3361 ccaccggcgg gctgatggac atgtcgttgt cgttgagcac caccagcagg cgggcgtcgc
     3421 gctcgacccc ggcgttgttg agcgcctcga aagccatgcc ggcggtcatg gcgccgtcgc
     3481 cgatgatggc caccgcgtgg cggtcctcgc ccttctgtcg cgccgccagc gccatgccca
     3541 gcgcggccga gatgctggtg gacgaatgcg cggtgccgaa ggtgtcgtac tcgctttcgt
     3601 cgcggcgcgg aaagccggac atgccgccca gctgacgcaa cgtgggcatg cgctcgcgtc
     3661 gcccggtcag gatcttgtgc ggataggttt ggtggccgac gtcccacacc agccggtcgt
     3721 gtggcgtctg aaacacgtag tgcagcgcaa tggtgagctc caccgtgccc aggttggagc
     3781 tcaggtggcc gccggtgcgg gccacgttgg tcagcacgca tgcgcgcagt tcgtccgcca
     3841 ggcccttgag ctgcgcgcgc ggcaggcggc gcagatcggc cgggctgtgg atggattcga
     3901 gcagggcttg ggacatcgtc agttctcgcg cttgaccacg cggtcggcca gccagcgcaa
     3961 cggcgccagc gcagtcaggc cgctggcgtc gagcgcctgg tgcgcctggg tgtgcaactg
     4021 cgccgccagg gcctgggcct gggtcagtcc cagcagggac acgtaggtgg gcttgtcggc
     4081 cgcggcatcc ttgccggcgg tcttgcccag ttgggcggaa tcggcggtga cgtcgaggat
     4141 gtcgtcgacc acctgaaagg ccagaccaag cgccgcgccg tactggtcca gcgcggcccg
     4201 ggtgtgggcc ggcagcgccg gcgtggcgca ggacgcgccc atgtgcacgg cggcctgcag
     4261 cagggcgccg gtcttcagcc ggtgcatggc ctgcagctgc tcctcggtca gcacgcgccc
     4321 cacgctgtcc aggtcgatgg cctgacctcc cgccatgccg gtcgcaccgg cggcgcgcgc
     4381 cagcaggcca cacagtgccg cctgcatgga ggccggcacc tcatcgccct cgggggtcag
     4441 cagctcgaac gccagcgcct gcaaggcgtc gccggccagc agggcctggg cctggccgaa
     4501 gcgcacatgc accgtcggct tgccacggcg aagcacgtcg ttgtccatgc atggcatgtc
     4561 gtcgtgcacc agggagtagg cgtggatcaa ttccaccgcg caggccgcgc gcagcgccgc
     4621 tgcggcgtgc ccctgcaccg cctcggcggc ggccagcacc agcagcggcc gcaggcgctt
     4681 gccgccgtcg agcacggcgt agcgcatggc ctcgcccagg ccggcgggcg catcgaccgg
     4741 tacccagtcc accagcgcct gctcgacgcg cgtcagcagc ctgtcggacc aggtcgggaa
     4801 atcgcgcgcg ttcactccgg catccacggt ttcacgtcac cgcgctcgtc cagcacgcgc
     4861 acctggtctt cgacggcctg caggcggtcg cggcagtagc gcagcagctc cgtgccacgc
     4921 tggtaatggc tcagcagatc ttccagcggc agctggccgg attcgagccc ggcgatcagt
     4981 tgttcaagct gctgcaccgc ctcgccatag ctggcaggct gggccggagc ggaagcggct
     5041 tttcggctca tgtgcgcgaa caaaagcgcc tattttaggc ggcggcggca aggcgcgggc
     5101 cgcccgtcgc ccaggccacc ggcccaccga ccgatccgcc cggggccagc ccggggccgg
     5161 ccgcggcaag gcggctaaaa taaaaggttc cccggcgcgc gtggcgcgcg ggttcgcgcg
     5221 cgcaatatcc tcggatgcca ggcgcttact tcctgcccct tcataggggg agtcttcaac
     5281 atcggcacac catgtctgat ttgagtcttc agttacagca ggcggcaagt caacttcccg
     5341 tccattccta tttcgatccg gcgctgtacg agcgcgaaaa gaagctgctc ttcgagtccg
     5401 gcccgcgcta cgtggggcac gcgctggcgg tacccgaagc ccacgactat ttcgtgctgc
     5461 cgcaggaagc cggcgggcgc gcgctgctgc gcgacgcgca gggccagatc cagctggtct
     5521 ccaactgctg ccgccaccgc caggccatca tgctgggcgg gcgcggcaac ctgctgaccg
     5581 aaggcaaggg ccatgcgggc ggcaacatcg tctgccccat ccaccgctgg acctattcca
     5641 gccgcggcga gctgctgggc gcaccgcact tcgagcgcga cccctgcctg aacctcagca
     5701 actacccgct gcgccagtgg aacggtctgc tgttcgagga caacggccgc gacatcgccg
     5761 ccgacctggc cggcatgacc accgccaagg acctgtcgtt cgacggctac gtgctcgatc
     5821 acgtcgaact gcacgagtgc aactacaact ggaagacctt catcgaggtc tacctggagg
     5881 actaccacgt cggccccttc caccccggcc tgggcaactt cgtcacct
//
LOCUS       DAOQTZ010000142         5907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_14885_length_5907_cov_12.405673, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000142 DAOQTZ010000000
VERSION     DAOQTZ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14885_length_5907_cov_12.405673"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..689)
                     /locus_tag="PKN51_12445"
     CDS             complement(171..689)
                     /locus_tag="PKN51_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNT85951.1"
                     /translation="MPAVRVRIQTEDFDLAAEVAALRAGDARVGAVCAFVGTVRDRHA
                     PALAAAPHADGGASPVSALELEHYPGMTEKAIEAMIDAAQQRFDILGARVVHRVGLLQ
                     PLDQIVLVAVTSMHRHESFAACEFIMDYLKTQAPFWKKESTPEGAHWVDARVADDAAL
                     ARWGIRAGNAAR"
     gene            complement(695..988)
                     /locus_tag="PKN51_12450"
     CDS             complement(695..988)
                     /locus_tag="PKN51_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNT85952.1"
                     /translation="MNAPDRAQAQQPLRTVTVRYFAAIREALGTGSERLTTPAATLSA
                     LRDELIARGGAHAETLARGKAVRMALDQLMVSDEAAPLADGAEVAFFPPVTGG"
     gene            complement(985..2238)
                     /locus_tag="PKN51_12455"
     CDS             complement(985..2238)
                     /locus_tag="PKN51_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNT85953.1"
                     /translation="MSDTPRRPPLMPLDDALARLLAAAAPLGRTERVDTFDADGRVLA
                     QDLVSALQVPPQDNSSMDGYAVRSADVTAAGVELPVSQRIAAGHAGAPLAPGSAARIF
                     TGAPMPAGADAVVMQEDAESLDDGARVRLNAVPAPGQWVRAAGEDVTRGRAVLQQGER
                     LAPASIGLAAGIGMASLQVAARPRVALFSTGDELVMPGAVPPEAMKPGAIYNSNRFFL
                     RALLQRLGCTVTDLGIVPDDRAATLEALRQAADGHDLILTSGGVSVGEEDHIKPAVEQ
                     LGALDLWQIAMKPGKPFAYGHVRRAGGGQAHFMGLPGNPVSSYVTFLLLVRPFLLRLQ
                     GAREVALPSLALRADFTLDKADRRREFLRARRNAQGGLELFGNQSSGVLTSAHWADGL
                     IDLPAGQTIAAGDMVRFIPVRELLA"
     gene            complement(2231..2773)
                     /gene="mobB"
                     /locus_tag="PKN51_12460"
     CDS             complement(2231..2773)
                     /gene="mobB"
                     /locus_tag="PKN51_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNT85954.1"
                     /translation="MKVVGFAGYSGSGKTTLVEQLIARLRARGLRVSVVKHAHHRFDI
                     DHPGKDTFRHREAGAFEVAIASSRRLALMREFERPTELSVHHLIAELWDGVDWVLVEG
                     FRGSDLLKVEVWRPATGHPARYPDDDFVVAIATDAPAALPVPTLRPVLDLNDAEAIAQ
                     WLIDNGERFDYDPERYLPHV"
     gene            complement(2844..4271)
                     /gene="thrC"
                     /locus_tag="PKN51_12465"
     CDS             complement(2844..4271)
                     /gene="thrC"
                     /locus_tag="PKN51_12465"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNT85955.1"
                     /translation="MLYLSTRGDSTPRRFCDILLEGLAPDGGLYLPEHYPQIDAARLA
                     ALRRTHAEGGYAALAFEILSLYIDDIPADDLRAICQRTYTAEVFGTPEIVPLKPLHQG
                     FYLEALSNGPTLAFKDMAMQLLGQLFEYELGRRGQTLNILGATSGDTGSAAEYAMRGK
                     RGIRVFMLSPHGRMSAFQQAQMFSLQDPNIHNIAIEGVFDDCQDIVKAVSNDLEFKRK
                     YRIGTVNSINWARLLAQVVYYFAGYFQATRSDGERVSFTVPSGNFGNVCAGHVARQMG
                     LPIAQLVVATNENDVLDEFFRTGVYRVRGAADTHETSSPSMDISKASNFERFVFDLLG
                     RDGARCKALFDDALRQHGQFDLSGDARFADAAARYGFVSGRSTHADRLATIRRVFAEH
                     GVTIDTHTADGVKVAREHLQPGVPMIVLETALPIKFAATIEEALGQPPARPARFDGIE
                     QLPRRVRVLPADAAQVKALITAECE"
     gene            complement(4312..5637)
                     /locus_tag="PKN51_12470"
     CDS             complement(4312..5637)
                     /locus_tag="PKN51_12470"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNT85956.1"
                     /translation="MKPIQVGLLGIGTVGSGTYQVLARNQEEIRRRAGRGIEITMVAD
                     LNTERARQLVGPGVKVVSDAREVIANPEIEIVIELIGGYGIARTLMMEAIAAGKHVVT
                     ANKALLAVHGSEIFQAAHDRGVMVAFEAAVAGGIPIIKALREGLTANRIQWIAGIING
                     TTNFILSEMRDKGLDFDVVLKEAQRLGYAEADPTFDIEGVDAAHKATLMSAIAFGIPV
                     QFDKAHVEGITKLSATDIRYAEQLGYRIKLLGITKRREGQGVELRVHPTLVPAKRLIA
                     NVEGAMNAVVVNGDAVGTTLYYGKGAGSEPTASAVIADLVDVTRLATSDPQHRVPHLA
                     FQPGQMRDVGVLSMEQVVTGYYLRLRVVDETGVLARVATILAEGGISIDALLQREADE
                     VGGEGSTQTDLIILTHDAREGAMNQALERLQALPSVLAPIVRIRKEELN"
     gene            complement(5677..>5907)
                     /locus_tag="PKN51_12475"
     CDS             complement(5677..>5907)
                     /locus_tag="PKN51_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85957.1"
                     /translation="RQFFMEVLRATRVMLVQGSGFNFPDQQHFRIVFLPHEDDLREAI
                     GRLAKFLEQYRARHARAAPVLDIATKKEAARA"
BASE COUNT          862 a         2023 c         2082 g          940 t
ORIGIN      
        1 caacgccgca tggcgcggcg agcgcggtgc gggtgggtgc gtagcgtgct cacccaagag
       61 gtggtggcga tcgcagccgc gagaccccgc atcgatgcgg cccgcccgaa gttgaccagc
      121 ggtcaactgc cgtttctagg gtcatcggtg gtcgcaggcg caagcccgca tcaacgcgcc
      181 gcgttgcccg cgcggatgcc ccagcgcgcc agcgccgcgt cgtcggcgac gcgcgcatcg
      241 acccagtgcg cgccctcggg cgtggactcc tttttccaga acggcgcctg ggtcttgagg
      301 tagtccatga tgaattcgca ggcggcaaag ctttcgtgcc ggtgcatgga cgtgacggcc
      361 accagcacga tctgatcgag cggctgcagc agccccacgc ggtgcaccac gcgcgcgccc
      421 aggatgtcga agcgctgctg cgcggcgtcg atcatggctt cgatggcctt ttcggtcatg
      481 ccggggtagt gttccagctc gagcgccgac accggcgacg cgccgccgtc agcgtgcggc
      541 gcggccgcca gcgcgggggc atggcgatcg cgcaccgtgc cgacgaaggc gcagacggcg
      601 cccacgcggg cgtcgccggc gcgcagcgca gccacctcgg ccgcgaggtc gaaatcttcg
      661 gtctggatgc ggacgcgcac ggcgggcatg gcgctcaccc gccggtcact ggcggaaaga
      721 aggccacctc ggcgccgtcg gccagcggcg ccgcttcgtc gctcaccatc agctggtcga
      781 gcgccatgcg cacggccttg ccgcgcgcca gcgtctcggc atgggcgccg ccgcgcgcga
      841 tcagctcgtc gcgcagcgcg ctcagcgtgg cggctggggt ggtcaggcgt tcgctgccgg
      901 tgcccagcgc ctcgcggatg gcggcgaaat agcgcacggt gacggtgcgc agcggttgct
      961 gcgcctgggc gcggtccggt gcgttcatgc cagcagctcc cgcaccggga tgaagcgcac
     1021 catgtcgccg gcggcgatgg tctggccggc cggcagatcg atcaggccgt cggcccagtg
     1081 ggccgaggtc agcacgcccg agctctggtt gccgaacagc tccagcccgc cctgggcgtt
     1141 gcgccgcgca cgcaggaatt cgcgccgccg gtcggccttg tccagcgtga agtcggcgcg
     1201 cagcgcaagc gacggcagcg ccacctcgcg cgcgccctgc agccgcagca ggaacggccg
     1261 caccagcagc agaaaggtga cgtagctgga caccgggttg cccggcaggc ccatgaaatg
     1321 cgcctggccg ccgcccgcgc gccggacgtg gccataggcg aacggcttgc cgggcttcat
     1381 ggcgatctgc cacagatcca gtgcgcccag ctgttcgacg gcgggtttga tgtggtcttc
     1441 ctcgccgacg ctgacgccgc cgctggtgag gatcaggtcg tgcccatcgg cggcctggcg
     1501 cagcgcctcc agggtggcgg cacgatcgtc gggcacgatg cccaggtcgg tcacggtgca
     1561 acccaggcgc tgcagcagcg cgcgcagaaa gaagcggttg ctgttgtaga tggcgcccgg
     1621 cttcatggcc tcgggcggca ccgcaccggg catgaccagc tcgtcgccgg tggaaaacag
     1681 cgccacccgc gggcgcgccg ccacctgcag cgacgccatg ccgataccgg ccgccagccc
     1741 aatactggcg ggcgccagac gctccccttg ttgtagcacg gcgcggccgc gtgtgacgtc
     1801 ctcgccggcc gcgcgcaccc attggccggg cgcgggcacg gcgttcaggc gcacccgcgc
     1861 accatcgtcc agcgactcgg cgtcttcctg catcaccacc gcgtcggcgc cggccggcat
     1921 gggcgcgccg gtgaagatgc gcgccgcact gcccggtgcc agcggcgcgc ccgcatggcc
     1981 ggccgcgatg cgctggctga ccggcagctc caccccggct gccgtcacgt ctgctgagcg
     2041 caccgcgtag ccgtccatcg agctgttgtc ctgcggcggc acctgcagcg ccgacaccag
     2101 gtcctgcgcc agcacgcggc catcggcgtc gaaggtgtcg acccgttcgg tgcgccccag
     2161 cggcgcggcc gccgccagca ggcgggccag cgcgtcgtcc agcggcatca ggggcggtcg
     2221 gcgcggggtg tcagacatgg ggcaggtacc gttcggggtc gtagtcgaaa cgctcaccat
     2281 tgtcgatcag ccattgggca atggcctcgg catcgttcag atccagcacc gggcgcaggg
     2341 tcggcacggg cagcgcggcc ggtgcgtcgg tggcgatggc gacgacgaag tcatcgtccg
     2401 gatagcgcgc agggtggccg gtggcggggc gccagacctc gaccttgagc aggtcgctgc
     2461 cgcgaaaacc ctccaccagc acccagtcca cgccgtccca cagctcggcg atcaggtggt
     2521 gcaccgacag ctcggtcggg cgctcgaact cgcgcatcag cgccagccgc cggctggagg
     2581 cgatcgccac ctcgaaggcg ccggcctcgc ggtggcggaa ggtgtccttg cccgggtggt
     2641 cgatgtcgaa gcggtgatgc gcatgcttga ccaccgacac gcgcagaccg cgcgcgcgca
     2701 gccgcgcaat cagctgctcg accagtgtgg tcttgcccga gccggagtag ccggcaaagc
     2761 ccacgacctt catcgcttgg tcacccgaat actatcaata tgatagctgc tggcgcttgt
     2821 cagttgtgcg ccagaggccg atttcattca cattctgcgg tgatcagcgc cttcacctgc
     2881 gcggcgtcgg cgggcaacac gcgcacgcgc cggggcagct gctcgatgcc gtcgaaccgg
     2941 gcgggccgcg cgggcggctg gcccagcgcc tcttcgatgg tggcggcaaa cttgatcggc
     3001 agcgcggtct ccagcacgat catcggcacg ccgggctgca ggtgctcgcg cgccaccttc
     3061 acgccgtcgg cggtgtgggt atcgatggtc acgccgtgct cggcaaacac gcggcggatg
     3121 gtggccaggc gatcggcgtg ggtgctgcgg ccactgacaa aaccgtagcg cgcggcggcg
     3181 tcggcaaagc gcgcgtcgcc gctcaggtcg aactggccgt gctggcgcag cgcgtcgtcg
     3241 aacagcgcct tgcagcgcgc gccgtcgcgc cccagcaggt cgaacacgaa gcgctcgaag
     3301 ttgctggcct tgctgatgtc catgctgggg ctgctggtct cgtgggtgtc ggcggcgccg
     3361 cgcacgcggt agacgccggt gcggaagaac tcgtcgagca catcgttttc attggtggcc
     3421 accaccagtt gcgcgatcgg cagccccatc tggcgcgcca cgtggccggc gcagacattg
     3481 ccaaagttgc cgctgggtac ggtgaagctg acgcgctcgc cgtccgagcg cgtagcttgg
     3541 aagtaaccgg cgaagtagta caccacctgc gccagcagcc gcgcccagtt gatggaattg
     3601 acggtgccga tgcggtactt gcgcttgaac tccagatcgt tgctgaccgc cttgacgatg
     3661 tcctggcagt cgtcgaacac gccttcgatg gcgatgttgt ggatgttcgg gtcctgcagg
     3721 ctgaacatct gcgcctgctg aaacgcgctc atgcgaccgt gcgggctgag catgaacacg
     3781 cggatgccgc gcttgccgcg catggcgtat tcggccgcac tgccggtgtc gccactggtg
     3841 gcacccagaa tgttgagcgt ctggccgcgc cggcccagct cgtactcgaa cagctggccc
     3901 agcagctgca tcgccatgtc cttgaaggcc agcgtggggc cgttggacag cgcctccagg
     3961 taaaagccct ggtgcagcgg cttcagcggc acgatctcgg gcgtgccgaa cacttcggcg
     4021 gtgtaggtgc gctggcagat cgcgcgcagg tcgtcggccg ggatgtcgtc gatgtagagc
     4081 gacaggatct cgaaggccag cgccgcgtag ccgccttcgg catgggtgcg gcgcagcgcc
     4141 gccagccggg cagcgtcgat ctgcgggtag tgctcgggca ggtacaggcc gccatcgggc
     4201 gccaagcctt ccagcaggat gtcgcagaag cggcgcggcg tgctgtcgcc gcgggtagac
     4261 agataaagca tggttcgctc gggcggtgtc acgcggcacg ggccgcggcg ttcagttcag
     4321 ctcttccttg cggatgcgca cgatcggtgc cagcacgctg ggcagggcct gcagccgctc
     4381 cagtgcctgg ttcatggcgc cctcgcgcgc atcgtgggtg aggatgatca ggtcggtctg
     4441 ggtgctgcct tcgccaccca cctcgtcggc ctcgcgctgc agcagcgcgt cgatgctgat
     4501 gccgccctcg gccaggatgg tggccacgcg cgccagcacg ccggtttcgt cgaccacgcg
     4561 caggcgcagg tagtagccgg tcaccacctg ctccatcgac agcacgccga cgtcgcgcat
     4621 ctgccccggc tggaaggcca ggtgcggcac gcggtgctgc gggtcgctgg tggccaggcg
     4681 ggtgacgtcc accaggtcgg cgatcacggc actggcggtg ggctcgctgc ccgccccctt
     4741 gccgtagtac agcgtggtgc cgacggcgtc gccgttgacc accaccgcgt tcatggcacc
     4801 ctcgacgttg gcgatcaagc gcttggccgg caccagggtg gggtgcacgc gcagctccac
     4861 gccctggcct tcgcgccgct tggtgatgcc cagcagcttg atgcggtagc ccagctgctc
     4921 ggcgtagcgg atgtcggtgg ccgacagctt ggtgatgccc tcgacatggg ccttgtcgaa
     4981 ctgcaccggg atgccgaagg cgatggcgct catcagcgtg gccttgtgcg ccgcgtccac
     5041 gccctcgatg tcgaaggtgg ggtcggcttc ggcgtagccc agtcgctgcg cctccttgag
     5101 caccacgtcg aagtccagcc ccttgtcgcg catctccgac aggatgaaat tggtggtgcc
     5161 gttgatgatg ccggcgatcc actggatgcg gttggccgtc aggccctcgc gcagcgcctt
     5221 gatgatgggg atgccgccgg ccacggcggc ctcgaaggcc accatcaccc cgcggtcgtg
     5281 ggcggcctgg aagatctcgc tgccgtgcac cgccagcagc gccttgttgg cggtgaccac
     5341 gtgcttgccg gcggcgatgg cttccatcat cagcgtgcgg gcgatgccgt agccgccgat
     5401 gagctcgatc acgatctcga tctccgggtt ggcgatcacc tcgcgcgcgt cgctgaccac
     5461 cttcacgccc gggccgacga gctgccgcgc gcgctcggtg ttcaggtcgg ccaccatggt
     5521 gatctcgatg ccacgaccgg cgcggcgccg gatttcttcc tggttgcgcg ccagcacctg
     5581 atacgtacca ctgccgacgg tgccgatacc gagcaggcct acctgaatgg gtttcatgtc
     5641 tgatttgtga atgaaaatgg cttcaaagcc cgtgcatcat gcgcgggcag cttctttttt
     5701 tgtagcaatg tcgagcaccg gggcagccct tgcgtggcgc gcccggtact gctccaggaa
     5761 cttcgccagc cggccgatgg cctcgcgcag gtcgtcttcg tgcggcagaa acacgatgcg
     5821 gaaatgctgc tggtccggaa agttgaagcc cgagccctgc accagcatca cgcgcgtggc
     5881 gcgcagcacc tccatgaaga actggcg
//
LOCUS       DAOQTZ010000143         5810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_15207_length_5810_cov_13.950304, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000143 DAOQTZ010000000
VERSION     DAOQTZ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15207_length_5810_cov_13.950304"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /gene="hisG"
                     /locus_tag="PKN51_12480"
     CDS             complement(<1..483)
                     /gene="hisG"
                     /locus_tag="PKN51_12480"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNT85958.1"
                     /translation="MITLALSKGRIFDETLPLLAAAGIEVLEDPETSRKLILPTNQDG
                     VRVVLVRATDVPTYVEYGGADLGVTGKDVLIEHGGAGLYQPLDLRIAQCHMAVAAPEG
                     FDYQRAVRQGSRITVATKYTTIARNFFADKGVHVDLIKLYGSMELAPLTGMADAIVDL
                     V"
     gene            complement(600..1874)
                     /gene="murA"
                     /locus_tag="PKN51_12485"
     CDS             complement(600..1874)
                     /gene="murA"
                     /locus_tag="PKN51_12485"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNT85959.1"
                     /translation="MDKLLIRGGRSLQGEVLISGAKNAALPEMCATLLSSAPVHLVNV
                     PRLHDVRTMRHLLDHMGVATQTHGERGGMSFVAPDDLNPEAPYELVKTMRAAVLVLGP
                     LLARFGRARVSLPGGCAIGSRPVDQHIKGLQAMGAQIEVEHGYMVARLPAGRSRLRGA
                     RITTDMVTVTGTENLLMAAALAEGETVLENAAQEPEITDLAELLIGMGARIEGHGTSR
                     IRIEGVERLHGATHEVVADRIEAGTFLCAVAATGGDVTLRHARADHLDAVIDKLRDAG
                     VQVEALDGAIRVRSAGGPALRAQSFRTIEYPGFPTDMQAQFMVLNCVAAGPSMVTETI
                     FENRFMHVNELARLGAQIHVDGRNAMIEGAARLSGATVMATDLRASASLVIAGLIAEG
                     ETVVDRIYHLDRGYDQMEEKLRALGADIERVR"
     gene            complement(1950..2192)
                     /locus_tag="PKN51_12490"
     CDS             complement(1950..2192)
                     /locus_tag="PKN51_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNT85960.1"
                     /translation="MNAEQIKQLIAAGLVCEHLELEGDGRHWFAVIVSPQFEGKRPIA
                     RHQMVYATLGSQLQTDAVHALSMKTLTPAEWAAQRS"
     gene            complement(2213..3016)
                     /locus_tag="PKN51_12495"
     CDS             complement(2213..3016)
                     /locus_tag="PKN51_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT85961.1"
                     /translation="MSGPRAPARLQALPAPEDGWKMLLKKEVLRFWRVALQTIAAPVL
                     TAVLYLLIFGHVLEGRVQVYPGVGYVAFLVPGLVMMSVLQNAFANSASSLVQSKIMGN
                     LVFVLLTPLSAWHWFVAYVGAAILRGLLVGLGVYLVTLWYAPPQVAALAWILVFAVLG
                     AALMGVLGLLAGLWSDKFDQMALFQNFIVMPMTFLSGVFYSIHSLPGVWQAISQVNPF
                     FYMIDGFRFGFFGVSDVSPWFSLGVAGTSVLLVSALTLHLLRTGYKLRY"
     gene            complement(3013..3951)
                     /locus_tag="PKN51_12500"
     CDS             complement(3013..3951)
                     /locus_tag="PKN51_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT85962.1"
                     /translation="MPAISFQSVSKVYPATGKPGSTPLQALDDVSFDIEEGEFFGLLG
                     PNGAGKTTLISILAGLARATAGRVLVHGHDVQTEFVSARRHLGIVPQELVFDPFFTVR
                     EALRFQSGYFGLHHNDDWIDELLEGLGLADKARANMRQLSGGMKRRVLVAQALVHKPP
                     VIVLDEPTAGVDVELRQTLWQFVDGLNRQGHTVLLTTHYLEEAEALCQRIAMLKSGRV
                     IALERTSTLLSNATANVLYFKTDGTLPAPLVAAARVTGRIVELPAHDPAEIERHLATL
                     REAGVKVEDLEIRKADLEDVFIELMNGQRAPAGSLA"
     gene            complement(4030..4335)
                     /locus_tag="PKN51_12505"
     CDS             complement(4030..4335)
                     /locus_tag="PKN51_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNT85963.1"
                     /translation="MLVLPSELLHAQAPACLTMLRDAARAEPGPLVLVDATPLVSFDS
                     SALAVLLECRRDCLLDGKQFAVHGLAPHLRELARLYGVSALLAPPQAAPSPALAPAD"
     gene            complement(4344..5009)
                     /locus_tag="PKN51_12510"
     CDS             complement(4344..5009)
                     /locus_tag="PKN51_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT85964.1"
                     /translation="MPTPLSLVRRRFLRQAGAALVALATGAAMPWARAADEAPDAMIG
                     RLSGEVLERLRNDKALRNGDMARVMAVVDSQVMPNVNFTRMTASATGPGWRRATPEQR
                     QKLQQEFKTLLVHTYAGALRQVSDQTVEVRPLRVAADDAEVVVRTWVKGRGEPVQLDY
                     RLERTPGQGSGWKIYDLNVLGVWLVDNYRPQFAQQINAGGIDALIRSLAERNQANAGS
                     SRG"
     gene            complement(5136..>5810)
                     /locus_tag="PKN51_12515"
     CDS             complement(5136..>5810)
                     /locus_tag="PKN51_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNT85965.1"
                     /translation="PRDPWEAYNRSMYRFNDAIDTAVLKPVATVYRDALPQPVRTGVG
                     NFFGNLGDVWSFVNQTLQAKPEAALHSFWRVVVNTTMGLGGVLDPASEMRLERRREDF
                     GQTLGHWGVPAGPYFVLPFFGPSTVRDTVALPVDIYGHPLSHVNDVSARNALGATQVV
                     NTRAAVLGATELLESAALDPYSFQRDAYLQKRRNDIHDGNPPADQERYDLDEPAPAGG
                     AAAAGR"
BASE COUNT          924 a         1996 c         2008 g          882 t
ORIGIN      
        1 gaccagatcg acgatggcgt cggccatgcc ggtcagcggc gccagttcca tgctgccgta
       61 gagcttgatc aggtccacat gcacgccctt gtcggcaaaa aagttgcgcg cgatggtggt
      121 gtacttggtg gccacggtga tgcggctgcc ctggcgcacg gcgcgctggt agtcgaagcc
      181 ctcgggcgcg gccacggcca tgtggcactg cgcgatgcgc aggtccagcg gctggtacag
      241 gccggcgccg ccgtgctcga tcagcacgtc cttgccggtc acacccaggt cggcgccgcc
      301 gtactcgacg taggtcggca cgtcggtggc gcgcaccagc accacgcgca cgccgtcctg
      361 gttggtgggc aggatcagct tgcgcgaggt ttcgggatcc tccagcacct cgatgccggc
      421 ggcggccagc agcggcaggg tctcgtcgaa gatgcggcct ttggacaggg caagggtgat
      481 cattcaggac tcgcagactg gccgtacgcg cagggcgcga aacctacagc cgaattgagg
      541 gatcgatgcc gcgccttgcg cgtctcctgc gggtgctgcg cgtccggtag gcatggggct
      601 cagcgcacgc gctcgatgtc ggcgcccagg gcgcgcagct tttcttccat ctgatcgtaa
      661 ccgcggtcca ggtggtagat gcgatcgacc acggtctctc cctcggcgat cagaccggcg
      721 atcaccaggc tggccgaggc gcgcagatcg gtcgccatca cggtggcgcc ggacaggcgc
      781 gccgcgccct cgatcatggc gttgcggcca tcgacgtgaa tctgcgcccc caggcgcgcc
      841 agctcgttga cgtgcatgaa gcggttttca aagatggtct cggtgaccat gctggggccg
      901 gccgccacac agttgaggac catgaactgc gcctgcatgt cggtcggaaa gcccgggtat
      961 tcgatggtgc gaaagctctg cgcgcgcaac gccggcccgc ccgcgctgcg cacgcggatg
     1021 gcgccgtcca gcgcctcgac ctgcacgccg gcgtcgcgca gtttgtcgat caccgcgtcc
     1081 aggtggtcgg cacgcgcatg gcgcagcgtg acgtcaccgc cggtggcggc gaccgcgcac
     1141 agaaaggtgc cggcctcgat gcggtcggcc accacctcgt gcgtggcgcc gtgcaggcgc
     1201 tcgacgccct cgatgcgaat gcggctcgtg ccatggccct cgatgcgcgc gcccatgccg
     1261 atcagcagct cggccaggtc ggtgatttcg ggttcctggg cggcgttttc cagcaccgtc
     1321 tcgccttcgg ccagcgcagc ggccatgagg aggttctcgg ttccggtgac ggtcaccatg
     1381 tcggtggtga tgcgcgcgcc gcgcaggcgg ctgcggccgg ccggcaggcg cgccaccatg
     1441 tagccgtgct cgacctcgat ctgcgcgccc atggcctgca ggcccttgat gtgctggtcc
     1501 accggccgcg agccgatggc gcagccaccc ggcagcgaca cccgcgcgcg gccaaagcgc
     1561 gccagcagcg ggcccagcac cagcaccgcc gcgcgcatgg tcttgaccag ctcgtacggc
     1621 gcctcggggt tgaggtcatc gggagcgacg aagctcatgc cgccgcgctc gccgtgggtc
     1681 tgcgtggcca cacccatgtg gtcgagcagg tggcgcatgg tgcgcacgtc gtgcagacgc
     1741 ggcacgttga ccagatgcac cggcgcgctg ctgagcagcg tggcgcacat ctcgggcagc
     1801 gcggcgttct tggcgccgga gatcagcacc tcaccctgca gcgaacggcc accgcggatc
     1861 aacagtttgt ccatgctcaa gcgaatgaat gaagaaacag ggctctgggg cgctcctgac
     1921 aagcgccaca agctatcaat accatagtat caggaacgct gcgcggccca ttcggcgggg
     1981 gtcagggtct tcatcgacag cgcgtgcacg gcgtccgtct gcagctggct gcccagcgtc
     2041 gcatacacca tctggtgacg tgcgatcggc cgcttgccct cgaactgggg cgagacgatg
     2101 acggcgaacc agtggcgacc gtcgccctcc agttccaggt gttcgcacac caggccggcg
     2161 gcgatgagtt gcttgatctg ttctgcgttc atggcgggat gggttgaagg ggtcagtagc
     2221 gcagcttgta gccggtgcgc agcaggtgca gcgtcagcgc cgacaccagc agcacgctgg
     2281 tgccggccac ccccaggctg aaccagggcg agacgtcgct gacaccaaag aagccgaacc
     2341 gaaagccgtc gatcatgtag aagaacgggt tgacctggct gatggcctgc cacacgcccg
     2401 gcagcgaatg gatggagtag aacacgcccg acagaaaggt catgggcatg acgatgaaat
     2461 tctggaacag cgccatctgg tcgaacttgt ccgaccacag gccggccagc aggcccagca
     2521 cacccatcag cgcggccccc agcacggcaa acaccaggat ccaggccagc gccgccacct
     2581 ggggcggcgc ataccagagg gtcaccaggt acacgcccag ccccaccagc aggccgcgca
     2641 ggatggccgc gccaacgtag gcgacgaacc agtgccaggc cgacagcggc gtcagcagca
     2701 cgaacaccag gttgcccatg atcttgctct gcaccaggct ggaggcgctg ttggcgaagg
     2761 cgttctgcag cacgctcatc atcaccagtc cgggcaccag aaaggccacg tagcccaccc
     2821 ccgggtacac ctgcacgcgc ccttccagca catggccgaa gatcagcagg tacagcaccg
     2881 ccgtcagcac cggcgcggcg atggtctgca acgccacgcg ccagaagcgc agcacctcct
     2941 tcttgagcag catcttccag ccgtcctccg gggccggcag ggcctgcagg cgggccggcg
     3001 cgcgcggccc gctcatgcca ggctcccggc gggggcgcgc tggccgttca tcagttcgat
     3061 gaacacgtcc tccagatcgg ccttgcggat ttccaggtct tcgaccttca cgccggcctc
     3121 gcgcagcgtg gccaggtggc gttcgatctc ggccgggtcg tgcgccggca gctcgacgat
     3181 gcgcccggtg acgcgcgccg ccgccaccag cggcgcgggc agcgtgccgt cggtcttgaa
     3241 atacagcacg ttggcggtgg cgttgctcag caaggtgctg gtgcgctcca gcgcgatgac
     3301 gcgcccgctc ttgagcatgg cgatgcgctg gcacagcgcc tcggcctctt ccaggtagtg
     3361 cgtggtcagc agcaccgtat ggccctgccg gttcaggccg tcgacgaact gccacagcgt
     3421 ctggcgcagc tccacgtcga cgccggcggt cggctcgtcg agcacgatca ccggcggctt
     3481 gtgcaccagc gcctgcgcca ccagcacgcg gcgcttcatg ccaccggaca gctgacgcat
     3541 gttggcccgg gccttgtcgg ccagacccag gccctccagc aattcgtcga tccagtcgtc
     3601 gttgtggtgc aggccgaagt accccgactg aaaccgcagc gcctcgcgca cggtgaagaa
     3661 agggtcgaac accagctcct gcggcacgat gcccagatgc cggcgcgccg acacgaactc
     3721 ggtctgcacg tcgtggccat gcaccagcac gcgaccggca gtggcgcgcg ccaggccggc
     3781 caggatggag atcagcgtgg tcttgccggc accgttgggg ccgagcaggc caaagaactc
     3841 cccttcctcg atgtcgaagc tgacatcgtc gagcgcttgg aggggagtgc tgccaggctt
     3901 gccggtggcc gggtagacct tggacaccga ctgaaacgaa atggcgggca taggcaaccg
     3961 gccattttac gttcttgccc gctgccgggc gggtgccggc acgcaagcat ccgggccacc
     4021 cgggggtatt cagtccgccg gagccagggc cggcgacggc gccgcctggg gcggcgccag
     4081 cagggccgac acgccgtaca gacgcgccag ctcgcgcaga tgcggcgcca gaccatgcac
     4141 ggcgaactgc ttgccgtcga gcaggcagtc gcgccggcat tccagcagca ccgccagcgc
     4201 cgaggagtcg aagctcacca gcggtgtggc atcgaccagc accagcggcc cgggctcggc
     4261 ccgcgcggcg tcgcgcagca tggtcaggca ggccggggcc tgggcgtgca gcagctcgga
     4321 cggcagcacc agcatggccc ggctcagcct ctggaactgc cggcgttggc ctggttgcgt
     4381 tcggccagcg agcggatcag ggcgtcgatg ccgccggcgt tgatctgctg cgcgaactgc
     4441 ggccggtagt tgtcgaccag ccagacgccc agcacattca ggtcatagat cttccagccg
     4501 ctgccctgac ccggcgtgcg ttcgagccga tagtccagct gcaccggctc gcctcgcccc
     4561 ttgacccagg tgcgcaccac cacctccgcg tcgtcggcgg cgacgcgcag cggccgcacc
     4621 tcgaccgtct ggtcgctgac ctggcgcagc gcgccggcgt aggtgtgcac cagcagggtc
     4681 ttgaactcct gctgcagctt ctggcgctgc tcgggcgtgg cccgccgcca gcccggcccg
     4741 gtggccgacg cggtcatgcg ggtgaagttg acgttgggca tgacctggct gtccaccacc
     4801 gccatcacgc gcgccatgtc gccgttgcgc agcgccttgt cgttgcgcag gcgctccagc
     4861 acctcgccgg acagccgccc gatcatggcg tccggcgctt cgtcggccgc cctggcccat
     4921 ggcatggccg cgcccgtggc cagcgcgacc agggcggcgc cagcctggcg cagaaaacga
     4981 cgacggacca aagacagggg ggtaggcatg gtggttattg ttcggtgctg ggccggacct
     5041 gatgacgacc ggctttggaa ggcatggtgc aacccgagtt cccgggcgca gttacaaatt
     5101 gtgccgcagc tgccggggcg gcgccgccct gccggctaac ggcctgccgc cgcggccccg
     5161 ccggcgggcg cgggttcgtc cagatcgtag cgctcctggt cggccggcgg attgccgtca
     5221 tggatgtcgt tgcggcgctt ttgcaggtag gcgtcgcgct ggaaggaata ggggtcgagc
     5281 gcggccgact cgagcagttc ggtggcaccc agcaccgcgg cgcgggtgtt gaccacctgg
     5341 gtggcgccca gcgcattgcg tgccgacacg tcgttcacgt ggctgagcgg gtggccgtag
     5401 atgtccaccg gcagcgccac cgtgtcgcgc acggtcgagg gcccgaagaa cggcagcacg
     5461 aagtacggcc cggcgggcac gccccagtgt cccagggtct gaccgaagtc ctcgcgccgg
     5521 cgctccaggc gcatctcgct ggccggatcg agcacgccgc ccaggcccat ggtggtgttg
     5581 acgaccacgc gccagaacga atgcagggcg gcttccggct tggcctgcag cgtctggttg
     5641 acgaacgacc agacgtctcc caggttgccg aagaagttgc ccacgccggt gcgcaccggc
     5701 tgcggcagcg cgtcgcggta gaccgtggcc acgggtttga gcaccgcggt gtcgatcgcg
     5761 tcgttgaagc ggtacatgct gcggttgtag gcctcccacg gatcgcgcgg
//
LOCUS       DAOQTZ010000144         5574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_16023_length_5574_cov_15.581265, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000144 DAOQTZ010000000
VERSION     DAOQTZ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16023_length_5574_cov_15.581265"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2267
                     /gene="ppsA"
                     /locus_tag="PKN51_12520"
     CDS             <1..2267
                     /gene="ppsA"
                     /locus_tag="PKN51_12520"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNT85966.1"
                     /translation="GVRVPTGFATTAHAFREFLKHEGLAERISARLAALDVDDVRALA
                     EAGAEIRAWVENQPFPADLEKAVRDAFAQLSGGNAQASFAVRSSATAEDLPDASFAGQ
                     QETFLNVVGIEDVLHKMKEVFASLYNDRAISYRVHKGFAHDVVALSAGVQRMVRSDLG
                     AAGVMFTIDTESGFDQVVFITSSYGLGETVVQGAVNPDEFYVHKPMLEAGKKALIRRN
                     LGSKLIQMVFATPEERAASGRLVKTCDVPAELRNRYSLSDAEVEQLAHYALVIEQHYG
                     RPMDIEWGKDGQDGQLYILQARPETVKSQQGSTELRYKLKGSSAVLAEGRAIGQKIGT
                     GPVRLVHNIAEMDKVQPGDVLVTDMTDPNWEPVMKKASAIVTNRGGRTCHAAIIAREL
                     GIPAVVGCGDATARLKDGTLVTVSCAEGDTGHIYDGLLETEVTEVRRGEMPPIATKIM
                     MNVGNPQLAFDFAQLPNEGVGLARLEFIINNNIGVHPKAILDYPQVDADLKKAVESVA
                     RGHASPRAFYVDKVAEGVATIAAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENP
                     MLGFRGAARYISDDFREAFAMECEALRRVRDDMGLTNVQIMVPFVRTLAQAERVVALL
                     ADKGLRRGVNDLKVVMMCEVPSNAILAERFLEHFDGFSIGSNDLTQLTLGLDRDSGLE
                     LLAADFDERDPAVEALIHQAIQACRAQGKYIGICGQGPSDHPDFARWLADEGIVSISL
                     NPDSVIDTWTLLSQ"
     gene            2289..4604
                     /locus_tag="PKN51_12525"
     CDS             2289..4604
                     /locus_tag="PKN51_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HNT85967.1"
                     /translation="MSAPPAIKNEAPGAAWLRAALLLLWAAASFGVAFFARELNQVVA
                     GWPLNYWWLAQGGVLVFLGVVMLYAWWMGRAEPAPADEGATSVAAGSRAYIWRLHRQL
                     ALFTLGLVAFLLLLHWAERAGLSRGWIGPIFLFLSVMMYAAVGIAGRTTDPEEFYVAG
                     RRVPAMYNGMATAADWMSAASFISLAGALYLQGFSGTAAQPGGLAYVLGWTGGFCLVG
                     LLMAPRLRAMRLYTVPDFFAVRFGRGAQLLAAMGAVACSFMYVVAQIYGIGLIASRLT
                     GVQFEIGILLGLGGVLLCSFLGGMRAITWTQVVQYVVLLVAFLIPVSWLAYKQLGTPV
                     ALLAYGQQLSKIERLERTLIDAPAEQQVRQHYRERARDYAERLRDPAAALASERSALE
                     ERIRTLRAQGADFSFVMMARRELSALPKDEASARELWTRAMLDAQERAKPLGGLPRHG
                     QAFAGDPDGTPDERAEFNTSRLNFLALMFCLMVGTASLPHLLTRFYTTPSVAATRHSV
                     AWSLLFIAMLYLATPALAALVKYEVMQHLVGSSFDDLPNWIAQWARVDPALISVQDVN
                     GDRILQFGEIRLGADMIMLATPELGGLPYVVSGLVAAGGLAAALSTADGLLLTISNAL
                     VHDTAPMRGGRVWVSDNRVILSKFALLAVAMVAAATAALKTGEIISLVTASFSLAAAT
                     FFPALTLGLFWRGATRRAAVAGMLTGIAVTVGYMLQASAVLRAWWGLAPGGLWWGIHP
                     VSAGFFGVPAGFAVIAAVSAWEHWRGRRQRP"
     gene            complement(4739..5311)
                     /locus_tag="PKN51_12530"
     CDS             complement(4739..5311)
                     /locus_tag="PKN51_12530"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNT85968.1"
                     /translation="MHRHALTARDFAADAPQVARTLIGATLLVDGVGGIIVETEAYDR
                     ADAASHTHRGPTPRNAAMFGPPGHAYVYRSYGIHWCLNLVCREAGHGAGVLIRALQPT
                     HGLDTMRRRRGLQDERLLCAGPGRVGQALGITLAHYGLAVDAPPFELRAAPHGLADQV
                     VCGPRIGISRALELPWRFGLAGSRYLSRRF"
BASE COUNT          828 a         1843 c         1977 g          926 t
ORIGIN      
        1 agggcgtgcg cgtgcccacc ggctttgcca ccacggcgca cgccttccgc gaatttctga
       61 agcacgaagg cctggccgag cgcatcagcg cccgcctggc ggccctcgat gtggacgacg
      121 tgcgcgcgct ggccgaggcc ggcgccgaga tccgcgcctg ggtggaaaac cagcccttcc
      181 ccgccgacct ggaaaaggcc gtgcgcgacg cctttgccca gctgtccggc ggcaacgccc
      241 aggccagctt tgccgtgcgc agctcggcca cggccgagga tctgcccgac gcctcgtttg
      301 ccggccagca ggagactttc ctgaacgtcg tcggcatcga ggacgtgctg cacaagatga
      361 aggaggtgtt cgcctcgctg tacaacgacc gcgccatctc ctaccgcgtg cacaagggct
      421 ttgcccacga cgtggtggcg ctgtcggcgg gcgtgcagcg catggtgcgc tcggacctgg
      481 gcgcggccgg cgtgatgttc accatcgaca ctgaaagcgg tttcgaccag gtggtcttca
      541 tcaccagcag ctatggcctg ggcgagaccg tggtgcaggg cgccgtcaac ccggatgagt
      601 tctatgtgca caagcccatg ctcgaggccg gcaagaaggc gctgattcgc cgcaacctgg
      661 gcagcaagct gatccagatg gtgtttgcca cgcccgagga aagggcggcc agcggtcgcc
      721 tggtcaagac ctgcgacgtg ccggccgagc tgcgcaaccg ctactcactc agcgatgccg
      781 aggtcgagca gctggcgcac tacgccctgg tgatcgagca gcactatggc cgcccgatgg
      841 acatcgaatg gggcaaggac ggtcaggacg gccagctcta catcctgcag gcgcggcccg
      901 agacggtcaa aagtcagcag ggcagcaccg agctgcgcta caagctcaag ggcagcagcg
      961 cggtgctggc cgaaggccgt gccatcggcc agaagatcgg caccggtccg gtgcgcctgg
     1021 tgcacaacat cgccgaaatg gacaaggtgc agccgggcga cgtgctggtg accgacatga
     1081 ccgacccgaa ctgggagccg gtgatgaaga aggccagcgc catcgtcacc aaccgcggcg
     1141 ggcgcacctg ccacgccgcc atcatcgcgc gcgagctggg cattccggcg gtggtcggct
     1201 gcggcgacgc caccgcgcgc ctgaaggacg gcacgctggt gacggtgagc tgcgccgaag
     1261 gcgacaccgg ccacatctac gacggtctgc tggaaaccga ggtcaccgag gtgcgccgcg
     1321 gcgagatgcc gccgatcgcc accaagatca tgatgaacgt gggcaacccg cagctggcgt
     1381 tcgactttgc ccagctgccg aacgagggcg tgggtctggc gcggctggag ttcatcatca
     1441 acaacaacat cggcgtgcac cccaaggcca ttctcgacta cccgcaggtc gacgccgacc
     1501 tgaagaaggc cgtggagagc gtggcgcgtg gccacgccag cccgcgcgcc ttctacgtcg
     1561 acaaggtggc cgagggcgtg gcgacgattg ccgccgcctt ctggcccaag ccggtgatcg
     1621 tgcgcctttc ggacttcaag agcaatgaat accgcaagct gatcggcggc agccgctacg
     1681 agccggaaga agaaaacccg atgctcggct ttcgcggtgc cgcgcgctac atcagcgacg
     1741 atttccgcga agccttcgcc atggaatgcg aggcgctgcg ccgcgtgcgc gacgacatgg
     1801 gcctgaccaa cgtgcagatc atggtgccct tcgtgcgcac gctggcgcag gccgagcggg
     1861 tggtggcgct gctggccgac aaggggctgc ggcgcggtgt caacgacctc aaggtggtga
     1921 tgatgtgcga ggtgcccagc aacgccatcc tggccgagcg cttcctggag catttcgacg
     1981 gcttttccat cggctcgaac gacctgaccc agctcacact ggggctggat cgcgattccg
     2041 gtctggaact gctggcggcc gacttcgacg agcgcgatcc ggccgtggag gcgctgattc
     2101 accaggccat ccaggcctgc cgggcgcagg gcaagtacat cggcatctgc ggtcagggcc
     2161 ccagcgacca cccggacttc gcgcgctggc tggccgacga gggtatcgtc tcgatttcgc
     2221 tcaaccccga cagcgtgatc gacacctgga ccttgctgtc acaataaaaa acccgtccat
     2281 cgcacgccat gtcagcgcca ccagctatca aaaatgaagc tcctggcgcg gcctggctgc
     2341 gtgccgcgct gctgctgttg tgggcggccg cgtcgttcgg tgtggccttt ttcgcgcgcg
     2401 agctgaacca ggtcgtggcc ggctggccgc tcaattactg gtggctggcg cagggcgggg
     2461 tgctggtgtt cctcggcgtg gtgatgctgt atgcgtggtg gatgggccgc gccgagcccg
     2521 cgcctgccga cgagggggcg actagcgtgg ctgccgggtc gcgcgcctac atctggcggt
     2581 tgcatcgtca gctggcgctg ttcaccctgg gcctggttgc ctttctgctg ctgctgcact
     2641 gggccgagcg cgccggtctg tcgcgcggct ggatcgggcc gatcttcctg ttcctgtcgg
     2701 tgatgatgta cgcggcggtg ggcattgccg ggcgcaccac cgacccggag gagttctacg
     2761 tcgccggccg gcgcgtgccg gccatgtaca acggcatggc cacggcggcc gactggatga
     2821 gcgccgcctc cttcatcagc ctggccgggg cgctgtacct gcagggcttt tccggaaccg
     2881 ccgcgcagcc cggtggcctg gcctacgtgc ttggctggac cgggggcttt tgtctggtcg
     2941 gtttgctgat ggcgccgcgc ctgcgcgcca tgcggctgta caccgtgccc gacttctttg
     3001 ccgtgcgatt cggtcggggg gcgcaactgc tggcggcgat gggggcggtg gcctgctcgt
     3061 tcatgtacgt ggtggcgcag atctacggca tcggtctgat cgcctcgcgc ctgaccgggg
     3121 tgcagttcga gatcggcatc ctgctgggcc tgggcggggt gctgctgtgc tccttcctgg
     3181 gcggcatgcg cgccatcacc tggacacagg tggtgcagta cgtggtgctg ctggtggcct
     3241 ttctgatccc ggtgtcctgg ttggcgtaca agcagctggg cacgcccgtg gccttgctgg
     3301 cgtacggcca gcagctgtcc aagatcgagc ggctggagcg cacgctgatc gatgcacccg
     3361 ccgagcaaca ggtgcgccag cactaccgcg agcgcgcccg cgactacgcc gagcgcctgc
     3421 gcgatccggc ggccgcgctg gccagcgagc gcagcgcgct ggaggagcgc atccgcacgc
     3481 tgcgcgccca gggtgcggac ttttccttcg tcatgatggc ccggcgcgag ctgtcggccc
     3541 tgcccaagga cgaggccagc gcgcgcgagc tgtggacgcg cgccatgctc gacgcgcagg
     3601 agcgcgccaa accgctgggt gggctgccgc gacacggtca ggcgtttgcc ggcgaccccg
     3661 acggcacacc ggacgagcgc gccgaattca acacctcgcg cctgaacttc ctggccctga
     3721 tgttctgcct gatggtgggt accgccagcc tgccgcacct gctgacgcgc ttctacacca
     3781 cccccagcgt ggcggcgacg cggcactcgg tggcctggtc gctgctgttc attgccatgc
     3841 tgtacctggc cacaccagcg ctggcggcgc tggtcaagta cgaggtgatg cagcatctgg
     3901 tgggcagcag cttcgacgat ctgcccaact ggattgccca gtgggcgcgg gtggatccgg
     3961 cgctgatttc ggtgcaggac gtcaacggtg accgcatcct gcagttcggc gaaatccgcc
     4021 tgggcgccga catgatcatg ctggccacgc ccgagctggg tgggctgccc tacgtggtgt
     4081 ccggcctggt ggcggccggc gggctggcgg cggcgctgtc caccgccgac ggactgctgc
     4141 tgaccatcag caatgcgctg gtgcacgaca ccgcgcccat gcgcggcggc cgcgtgtggg
     4201 tgtcggacaa ccgcgtgatc ctgtccaagt tcgccctgct ggcggtggcc atggtggcgg
     4261 cggcgacggc ggcgctcaag acgggcgaga tcatttctct ggtgacggcc tcgttttcgc
     4321 tggcggccgc caccttcttc ccggcgctca ccctcggcct gttctggcgc ggcgccaccc
     4381 ggcgcgccgc cgtcgccggc atgctgaccg gcatcgccgt caccgtcggc tacatgctgc
     4441 aggccagcgc ggtcctgcgc gcctggtggg gcctggcgcc gggcggtctg tggtggggca
     4501 tccacccggt gtcggccggc ttcttcggcg tgccggcagg ttttgccgtg atcgccgcgg
     4561 tgtcggcctg ggagcactgg cgcgggcggc gccagcgccc ctgatcgagc gccgcaaagc
     4621 tctcgattgc tattaataac gtagcttacg gcgctgactg ggtgcgcgct ggagcatgtt
     4681 ttttcctcaa acccgccaga atccaggttg gggccgggag cgccggtcga gggcctgatc
     4741 agaaccttcg gctcaggtag cgcgagccgg ccaggccaaa tcgccacggc agctccagcg
     4801 cccggctgat gccgatgcgc gggccgcaca ccacctgatc cgccagcccg tggggtgcgg
     4861 cccgcagctc gaacggcggc gcatccaccg ccagcccgta gtgcgccagc gtgatgccca
     4921 gcgcctgtcc cacacgaccg gggccggcgc acagcaggcg ttcgtcctgc aggccgcgac
     4981 gccggcgcat ggtgtccagg ccgtgcgtcg gctgtagggc gcggatcagc acgccggcgc
     5041 cgtgtccggc ttcgcggcac accaggttca ggcaccagtg gatgccgtag ctgcggtaca
     5101 cgtaggcatg gccgggcggg ccgaacatgg cggcgttgcg tggtgtcggg ccgcggtggg
     5161 tgtgcgaggc ggcgtcggcg cggtcgtagg cctcggtctc gacgatgatg ccgcccacgc
     5221 cgtccaccag cagagtggcg ccgatcaggg tgcgggcaac ctgcggtgca tcggcggcga
     5281 agtcgcgggc ggtgagggcg tggcggtgca tgacggtcgc cagtctatgg attcggcgtt
     5341 ggcatgtctt caataccgtt cgggctgagc ctgctcaagc ccttcgacag gctcaggatg
     5401 cacggttcaa atttcaggag ttcggaagcc atagggtctg ctcacaggcc ctggtgcagg
     5461 gtggcgctgc gcagggtgac gggctgagat gcacgcggtg ctcagcgctg caccgcaggc
     5521 agccgagtgg gcgcagtatg ctgagggact gcagatcgcg ccccaccccg cggt
//
LOCUS       DAOQTZ010000145         5289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_17188_length_5289_cov_13.154375, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000145 DAOQTZ010000000
VERSION     DAOQTZ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17188_length_5289_cov_13.154375"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKN51_12535"
     CDS             <1..471
                     /locus_tag="PKN51_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNT85969.1"
                     /translation="SMKDFYWDIRPKPEYGTIEVRVFDTPLTVERAAALAALVQSLAA
                     WIQRERPFQPSEDDYLVYSYNRFQACRFGLEAVYVEPASGRHLPLREHLLATFEQLAG
                     HAADCAASDALALLRADTEAGRNDARWLRERQAREQLLAEVIRQAALRFRGGQP"
     gene            527..1372
                     /locus_tag="PKN51_12540"
     CDS             527..1372
                     /locus_tag="PKN51_12540"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNT85970.1"
                     /translation="MTLRIGISARLLHKPPPGLGLPTKRLQFLESNMAHWIMGHGVVA
                     LMIPFIDQRLPTLRKRPPLRDLVEQLDGLVLQGGIDIHPQAYGAAPWQAQADYDPIRD
                     DYELELLHGFIGADKPVLGICRGCQLLNVYFGGTLVQDIPSQWPGAIAHSDTAHYDRL
                     AHEVHFMPGSRLADIYGYEPRRVTSIHHQCVDRLGRDLVLEARSPIDLVPEGIRHTGY
                     PFVLGVQWHPEFHLNGNGAAADILDSGPLMMAFLKAAARRAGRVRRLAEGVARVRERA
                     SALTR"
     gene            1391..2425
                     /gene="thiL"
                     /locus_tag="PKN51_12545"
     CDS             1391..2425
                     /gene="thiL"
                     /locus_tag="PKN51_12545"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNT85971.1"
                     /translation="MGEFELIDRHFKPKPVAARLQQASAAPDSIATPVVLGIGDDCAL
                     LQPAPGMQLAISTDMLVEGRHFLPDVAPARLGHKALAVNLSDLAACGAEPLACTLALA
                     LPPERARDDAWLAALAGGLWALADAQRCPLVGGDTTAGPLNLCLTVFGQVPAGQALRR
                     DGARVGDDVWVSGALGHARLALGALRGEWTLPPQLLAAARTRLECPTPRIALGLALRG
                     IAHSAADVSDGLAGDLGHILRASAVGACLDAQETMKTIAAGAFSASAEGLFDRQTLLR
                     LALTGGDDYELVFTAPPDARSAVQAAGQASATPLARIGRIEAEPGLRLLGADGRIEPL
                     AARAFDHFAP"
     gene            2547..4151
                     /locus_tag="PKN51_12550"
     CDS             2547..4151
                     /locus_tag="PKN51_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNT85972.1"
                     /translation="MTLIRYLCPSALAALWLALAPPAAAQGAAPASGGSRITAVKLYP
                     GSATVQRTLRLAAGARQALFACLPPGLDATSLQLSADAGVRVGELALRQQPRELLGPA
                     CASALDERIRTLEDRIAALAAETGGIDIATGYLKNFGAGGEPGRGTPAGQIGATAQAL
                     QQGGQTALLRRHQLARQQEQLERELKPLLAERDRASRGDQPVSTVQVTLWAPQGGELR
                     LSYQVRGPGWQPAYRASLDSTSRQLRLERLAQVAQSSGEDWSEVPLTLSTGQPGAATQ
                     GPLPRPWRVGIEPPPAAGEARQRLAAAPAPVPVAAAAKAPEAEPSFDVSVFQGSFATE
                     FVLPQRVSVPSSGERITLTLGEQVLDTRLRVRTTPALDAHAYLIASFTTPAGVWPAGP
                     LHLVRDGAFVGSARFDAASASRDGLAFGRDELVGVRVERPEQQQGTGGFIGSRHQRID
                     SRRYVIDNRHREPIVLELLDSAPVGEHADVRIESRYQPAPQTERWNEQKGVVAWEQSL
                     AAGASQSFSAEHVISWPSDAHLRERR"
     gene            complement(4312..4995)
                     /locus_tag="PKN51_12555"
     CDS             complement(4312..4995)
                     /locus_tag="PKN51_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNT85973.1"
                     /translation="MTTPATPPITEEDIAEFLIHTPGFFERHAELLTAVQLTSPHGGR
                     AVSLQERQAEMLRDKIKLLEQRLMEMMRHGNENMLIADRMLRWARQMFLVARPVDLPA
                     VLVSEIRSQFSVPQAALKLWDVAEVYAGEPFAQGASEDVRSLASSLGVPYCGVNAGFD
                     AVQWLDDPEAAASLALIPLRAGAAGPAFGLLILASPDSQRYQAGMATDFLERIAELAS
                     AALSRVRDD"
     gene            5127..>5289
                     /locus_tag="PKN51_12560"
     CDS             5127..>5289
                     /locus_tag="PKN51_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNT85974.1"
                     /translation="MRIPDWSPALKPQPAELVDAILARRGGQLINLDRALLWSEPLAR
                     GWNTYLKAVR"
BASE COUNT          751 a         2015 c         1842 g          681 t
ORIGIN      
        1 agcatgaagg acttctactg ggacatccgc cccaagcccg agtacggcac catcgaggtg
       61 cgggtgttcg acaccccgct gacggtggag cgcgccgccg ccctggccgc gctggtgcag
      121 agcctggcgg cctggatcca gcgcgagcgc ccgttccagc ccagcgaaga cgactacctg
      181 gtctacagct acaaccgctt ccaggcctgc cgcttcgggc tggaggcggt ctacgtcgag
      241 ccggccagcg gccggcatct gccgctgcgc gagcacctgc tggccacgtt cgagcaactg
      301 gccgggcatg ccgccgactg cgccgccagc gacgccctgg ccctgctgcg cgccgacacc
      361 gaggccggcc gcaacgatgc ccgctggctg cgcgagaggc aggcgcgcga acagttgctg
      421 gccgaggtga tccgccaggc ggcgctgcgc tttcgcggcg gtcaaccctg aggccgctgg
      481 cgcgttggcc ctacagtcgc cccatcgtca cgcacgcgcc cgccccatga ccctgcgcat
      541 cggcatttcc gcccgcctgc tgcacaagcc accgcccggc ctgggcctgc cgaccaagcg
      601 cctgcagttt ctggaaagca acatggccca ctggatcatg ggccatggcg tggtggcgct
      661 gatgatcccc ttcatcgacc agcgcctgcc gacgctgcgc aagcgcccgc cgctgcgcga
      721 cctggtggag cagctcgacg gcctggtgct gcagggcggc atcgacatcc acccccaggc
      781 ctatggcgcc gcaccctggc aggcgcaggc cgactacgac ccgatccgcg acgactacga
      841 gctggagctg ctgcacggct tcatcggcgc cgacaagccg gtgctgggca tctgccgcgg
      901 ctgccagctg ctcaacgtgt actttggcgg cacgctggtg caggacatcc ccagccagtg
      961 gccgggcgcc atcgcccaca gcgacaccgc ccattacgac cgtctggcgc atgaggtgca
     1021 cttcatgccc ggctcgcggc tggccgacat ctacggctac gaaccgcgcc gcgtcaccag
     1081 catccaccac cagtgcgtcg accgcctggg ccgcgacctg gtgctggagg cgcgcagccc
     1141 gatcgacctg gtgcccgagg gcatccgcca caccggctac ccgttcgtgc tgggcgtgca
     1201 gtggcacccc gagttccatc tcaacggcaa cggcgccgcc gccgacatcc tggacagcgg
     1261 cccgctgatg atggccttcc tgaaggccgc cgcacgccgc gccggccgcg tgcgccggct
     1321 ggccgagggc gtggcccgcg tgcgcgaacg cgccagcgcg ctcacccgct gagcgcgcgc
     1381 aggagccacc atgggcgaat tcgagctgat cgaccgtcat ttcaagccaa aaccggttgc
     1441 agcgcgcttg cagcaagcgt cagcagctcc tgattcaata gcaacgcccg tggtgctggg
     1501 gatcggcgac gactgcgccc tgctgcagcc ggcgcccggc atgcaactgg ccatcagcac
     1561 cgacatgctg gtcgagggcc gccacttcct gcccgacgtc gcgccggccc ggctgggcca
     1621 caaggcgctg gcggtcaacc tgagcgacct ggcggcctgc ggcgccgagc cgctggcctg
     1681 cacgctggcg ctggcgctgc cgcccgagcg cgcgcgcgac gacgcctggc tggcggccct
     1741 ggccggcggc ctgtgggccc tggccgacgc ccagcgctgc ccgctggtgg gcggcgacac
     1801 cacggccggc ccgctcaacc tgtgcctgac ggtgttcggc caggtgccgg ccgggcaggc
     1861 gctgcggcgc gacggcgcgc gcgtgggcga cgacgtctgg gtcagcggcg cgctgggcca
     1921 cgcccggctg gccctgggcg cgctgcgcgg cgaatggacg ctgccgccgc agctgctggc
     1981 cgccgcgcgc acgcggctcg agtgccccac gccccgcatc gcgctcggcc tggcgctgcg
     2041 cggcatcgcc cacagcgccg ccgacgtcag tgacgggctg gcgggcgatc tgggccacat
     2101 cctgcgggcg agcgccgtcg gggcctgcct ggacgcccag gaaactatga aaaccatagc
     2161 tgctggcgct ttctcggcaa gcgctgaagg cctttttgat cgtcaaaccc tgctgcgcct
     2221 ggcgctcacc ggtggcgacg attacgagct ggtcttcacc gccccgcccg acgcccgcag
     2281 cgccgtgcaa gccgccggcc aggccagcgc caccccgctc gcgcgcattg gccgcatcga
     2341 agccgaaccc ggcctgcgcc tgctcggcgc cgacggtcgc atcgagccgc tggccgcgcg
     2401 cgccttcgac catttcgccc cttgactgag cctttggatg tcttgaatac cgttcgggcc
     2461 gagctcgtcg aagcccatcg acaggctcag ggtgagcggt tccatgttcc agacagggag
     2521 ccataccaca ccgcatgaac cgcaccatga ccctgatccg atacctttgc ccatccgccc
     2581 tggccgcgct gtggctggcc ctggccccgc cggccgccgc ccagggcgcc gccccggcca
     2641 gcggcggctc gcgcatcacc gccgtcaagc tgtaccccgg cagcgccacc gtccagcgca
     2701 cgctgcgcct ggcggcgggc gcgcgtcagg cgctgtttgc ctgcctgccg ccggggctgg
     2761 acgccaccag cctgcagctc agcgccgacg ccggcgtgcg cgtgggtgag ctggcgctgc
     2821 gccagcagcc gcgcgagctg ctgggcccgg cctgcgccag cgcgctggac gaacgcatcc
     2881 gcacgctgga agaccgcatc gccgcgctgg cggccgaaac cggcggcatc gacatcgcca
     2941 ccggctacct gaagaacttc ggcgccggcg gcgagccggg gcgcggcacg cccgccggcc
     3001 agatcggcgc cacggcgcag gccctgcaac agggcggcca gacggcgctg ctgcggcgcc
     3061 accagctggc gcgccagcag gagcagctgg agcgcgagct caagcccctg ctggccgaac
     3121 gcgaccgcgc cagccggggc gaccagccgg tcagcacggt gcaggtcacg ctgtgggcgc
     3181 cgcagggcgg tgagctgcgc ctgagctacc aggtgcgcgg ccccggctgg caacccgcct
     3241 accgcgccag cctcgacagc accagccgcc agctgcgcct tgagcgcctg gcgcaggtgg
     3301 cgcaaagcag cggcgaggac tggagcgagg tgccgctcac cctgtccacc ggtcagcccg
     3361 gcgccgccac ccaggggccg ctgccgcggc cctggcgggt cggcatcgag ccgccgccgg
     3421 cggccggcga agcgcgccag cgcctggccg cggcccctgc cccggtgccg gtggcggccg
     3481 cggccaaggc gcccgaggcc gagccgagtt tcgacgtcag cgtgttccag ggcagcttcg
     3541 ccaccgaatt cgtgctgccg cagcgcgtca gcgtgccctc cagcggcgag cgcatcacgt
     3601 tgacgctggg cgagcaggtg ctggacaccc ggctgcgcgt gcgcaccacg ccggcgctgg
     3661 acgcccacgc ctacctgatc gccagcttca ccaccccggc cggcgtgtgg ccggccggcc
     3721 cgctgcacct ggtgcgcgac ggcgccttcg tcggcagcgc ccgcttcgac gccgccagcg
     3781 ccagccgcga cggtctggcg ttcgggcgcg acgaactggt cggcgtgcgc gtcgagcggc
     3841 ccgagcagca gcagggcacg ggcggcttca tcggcagccg ccatcagcgc atcgacagcc
     3901 gccgctacgt gatcgacaac cgccaccgcg agcccatcgt gctggaactg ctggacagcg
     3961 ccccggtggg cgagcacgcg gacgtgcgga tcgaatcgcg ctaccagccg gcgccgcaga
     4021 ccgagcgctg gaacgagcag aaaggcgtgg tggcctggga gcagtcgctg gccgccggcg
     4081 ccagccagag cttcagcgcc gagcacgtca tcagctggcc cagcgatgcg cacctgcgtg
     4141 agcggcgctg agatggggcc ggttcagtcg agggaccgat gacagtggac accctcgatc
     4201 atccaagcgc cgccaggtca tcggcagcag ccgtcgccga tggcgcggcg cgcgcaccgg
     4261 gccgctgaag gccagcgaag ccaccaggcc ctgccaccgc ggcttgccgg ctcaatcgtc
     4321 gcgcacccgc gacagcgccg cgctggcgag ctcggcgatg cgctccagga agtcggtggc
     4381 catgccggcc tggtagcgtt ggctgtcggg cgaggctagg atcagcaggc cgaacgccgg
     4441 accggcggcg ccggcgcgca gcgggatcag cgccagcgag gcggcggcct cgggatcgtc
     4501 gagccactgc acggcgtcga agccggcgtt gacgccgcaa tacggcacgc cgagcgacga
     4561 ggccaggctg cgcacgtcct cgctggcgcc ctgcgcgaac ggctcgccgg catagacctc
     4621 ggccacgtcc cacagcttga gcgccgcctg cggcacgctg aactggcttc tgatttcgct
     4681 caccagcacc gccggcagat cgaccgggcg cgccaccagg aacatctggc gcgcccagcg
     4741 cagcatgcgg tcggcgatca gcatgttctc gttgccgtga cgcatcatct ccatcaaccg
     4801 ctgctccagc agcttgatct tgtcgcgcag catttcggcc tggcgctcct gcaggctgac
     4861 ggcgcgcccg ccgtgcgggc tggtcagctg caccgcggtc agcaactcgg catggcgctc
     4921 gaagaagccg ggcgtatgga tcaggaattc ggcgatgtct tcttcggtga tcgggggcgt
     4981 ggcaggggtg gtcatgaatg ctccgcggat tcgaggatcg atgggccgcc gcaccggccg
     5041 tggccggcgc gcccggcacc cgcgcgggtg ccggcccatg gcgctggatt atcctcgcca
     5101 ccatcccaac gcccgcgagg ccgaccatgc gcattcccga ctggagcccg gcgctcaaac
     5161 cccagcccgc cgaactggtg gacgccatcc tggcacgccg cggcggccag ctcatcaacc
     5221 tggaccgggc gctgctgtgg agcgaaccgc tggcgcgcgg ctggaacacc tacctgaagg
     5281 cggtgcgca
//
LOCUS       DAOQTZ010000146         5262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_17296_length_5262_cov_10.833109, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000146 DAOQTZ010000000
VERSION     DAOQTZ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17296_length_5262_cov_10.833109"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1112
                     /locus_tag="PKN51_12565"
     CDS             180..1112
                     /locus_tag="PKN51_12565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT85975.1"
                     /translation="MLSLIIPVYRNEASIPELLTELQGLHQALTGALEVVFVVDGSPD
                     RCHALLRQQLPASGLNATLVLLTRNFGAFAAVRMGLEQARGDTFAVMAADLQEPPELV
                     LQMQAELARGQADVVVAERTLRHDPLLQRWQAQLFWAAYRRWVVPEMPPGGVDIFACN
                     RAFRDALLGLGESRSSLIAQIFWLGFRRASVPYERQPRRHGQSAWTWRKKLAYLADSV
                     FAFTDLPVRLLTRVGALGLLVAGVLGAVVLFGKLSGLIGVPGYAMTLIAILFFGALNL
                     FSLGIVGTYAWRAYENTKQRPQAVALRVDRFQSH"
     gene            1122..1583
                     /locus_tag="PKN51_12570"
     CDS             1122..1583
                     /locus_tag="PKN51_12570"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HNT85976.1"
                     /translation="MPASSVPSAPRVIDSRVTGVRLHELREVKDARGDLCVAEVGADL
                     PFAVRRSFLVYRVPSAELRGAHAHRRCGQFLQAVAGSVHVIADDGRQREEFCLDRPSL
                     GLYLPPMTWGIQHRYTQGAVLLVLASDPYDPADYIRDYDEFLALRRAGGAA"
     gene            1583..1954
                     /locus_tag="PKN51_12575"
     CDS             1583..1954
                     /locus_tag="PKN51_12575"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNT85977.1"
                     /translation="MAREALRYLLAGGANTALTYLLYLALLRLLDYRWAYVLAFLAGV
                     ALSFVLLRHVVFARPGKRFSWIYVTASHGVQLGLGLVVVEAWVAWLRGPAWLAPLAAA
                     LVCVPVIFVLQRWIFTPHAAS"
     gene            1941..>3662
                     /locus_tag="PKN51_12580"
     CDS             1941..>3662
                     /locus_tag="PKN51_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85978.1"
                     /translation="MPRPEHLRPALSKWWLRAGMALPLLMLGANLLAWLRWGTDLPYL
                     DDWRVYDDGSALSLDPRHLFRAVNNTISPVGLTLDVLAQRWLGGNPLPYQALSMLAVL
                     GGLLWLQWRLLGWAVRDRRQQALLFAFCVPMLQSGSYWGEQSLAYHQALPLLALLGAM
                     AWALPRPRRRAALGVFVLGAVAGLSYISGAIAALVLGACWLVLARVLRTHPTPALATR
                     IAGPGGALLLAGGLTSALQFHLTRGDAQARDRQYMGLTYPDSPDFWAFLAGKIGRATG
                     HGFASLPLELIWVALVLLALLAALGLTGRAALAPGRVPSVRRRRVAVVFLPLCAIVLT
                     YLVLVSLGRAGFRDPGIQGAGGVFRFGYERFHFFWVTLLFPWVAAALVPRRRTRAVPV
                     LVGTLLTALALAAVRGVFDVPSFYRSASAYRASELRCLGRQLGSGQPIRCPGYDMMGM
                     TDLTRAYIHARDIQASFVRYLPVQTRDGFGRELLDWHAAAGTEPGPWAHVRRRADGGL
                     QSEGDAQLTVAWPDAALSRRCRTLGVQLNIVTAHAETAQLYYRWSGQQSDSEAHSVRQ
                     AYQPDVDG"
     assembly_gap    3663..3762
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3763..4089
                     /locus_tag="PKN51_12585"
     CDS             <3763..4089
                     /locus_tag="PKN51_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT85979.1"
                     /translation="LGTVRLYGARGDAFSWGSWLLKAGLPPHCAIESALMTYHYGLWL
                     GFHSAYFEVRQDNSSVWRFHENFGATRIGADDRHFHYELAADALTQALQRYRRYLPHG
                     IRVSRD"
     gene            4121..4678
                     /gene="rfbC"
                     /locus_tag="PKN51_12590"
     CDS             4121..4678
                     /gene="rfbC"
                     /locus_tag="PKN51_12590"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNT85980.1"
                     /translation="MHVIPTALAGVLVLEPRVFGDDRGFFMESYNRRSFAQATGLDID
                     FVQDNHSRSRKGVLRGLHYQLRQPQGKLVRVTHGAVFDVAVDIRRGSPTFGRWVGVEL
                     SADNHRQLWVPPGLAHGFVVLSDSADFLYKTTDYYAPEHERSIAWDDPAIGIDWPLAA
                     HGITAPLLSAKDQAGQPLSRAELVD"
     gene            4700..>5262
                     /locus_tag="PKN51_12595"
     CDS             4700..>5262
                     /locus_tag="PKN51_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNT85981.1"
                     /translation="MNIAASIVSHGHGAPVEALLAQLAALGEPGLARVIVTFNVPEPA
                     SAERLRGRHWPFQLHLVDNPAPLGFGANHNRAFALDARIDGGQPLFAVLNPDLRCLVN
                     PFPPLVAELQSDPRVGAAYPVQTDARGCRQDHERLRPTPLRLLRRHLLGRRMEVGAGQ
                     APDWVNAAFLLLRRPAFEAIGGFDEAF"
BASE COUNT          625 a         1811 c         1809 g          917 t
ORIGIN      
        1 cactacccgc tggccgacca ccgtcagccg gtgcgtgccg cccagccgct gccgcacctg
       61 ccggtcaccg aggcggcctg tgccgaggtg ctgtcgctgc cctgctaccc tggactgacg
      121 gcgccgcagg gcgcgcgtgt catcgaggtc gtgcgccaat tcttcgcggc tgcctgacca
      181 tgctcagcct gatcatcccg gtctaccgca acgaggcttc gattccggag ctgctgaccg
      241 agctgcaggg gttgcaccag gcgctgaccg gcgcgctgga ggtggtgttc gtggtcgacg
      301 gcagccccga ccgctgccac gcgctgctgc gccagcagct gcccgccagc ggcctgaacg
      361 ccaccctggt gctgctcacg cgcaatttcg gcgcctttgc cgccgtgcgg atgggcctgg
      421 agcaggcgcg cggcgacacc tttgccgtga tggccgccga cctgcaggag ccgcccgagc
      481 tggtgttgca gatgcaggcc gagctggcgc gcggccaggc cgacgtggtg gtggccgagc
      541 gcaccctgcg ccacgacccg ctgctgcagc gctggcaggc ccagctgttc tgggccgcct
      601 accggcgttg ggtggtgccc gagatgccgc ccggcggcgt cgacatcttt gcctgcaacc
      661 gcgcctttcg cgacgccctg ctcggcttgg gtgaaagtcg cagctcgctg atcgcgcaga
      721 tcttctggct gggctttcgg cgcgccagcg tgccttacga gcgccagccg cgccgtcacg
      781 gccagtcggc ctggacctgg cgcaagaagc tggcctacct ggccgacagc gtgttcgcct
      841 tcaccgacct gccggtgcgc ctgctcacgc gcgtgggggc gctgggcctg ctggtggccg
      901 gcgtgctggg tgcggtggtg ctgttcggca agctttccgg gctgatcggt gtgcctggct
      961 acgccatgac gctgatcgcc atcctgttct tcggtgcgct caacctgttc agcctgggca
     1021 tcgtcggcac ctacgcctgg cgcgcctacg aaaataccaa gcagcgcccg caggccgtcg
     1081 ccttgcgggt cgatcgcttc cagtcccact agccgcacgt catgccagcg tcatccgtcc
     1141 ccagcgcgcc gcgcgtcatc gacagccgcg tcacgggggt gcgcctgcac gaactgcgcg
     1201 aggtgaagga cgcacgcggc gacctgtgcg tcgccgaggt cggcgccgat ctgccgtttg
     1261 ccgtgcgccg cagtttcctc gtctacaggg tacccagcgc cgaactgcgc ggcgcccacg
     1321 cgcaccgccg gtgcggccag ttcctgcagg cggtcgccgg ttcggtccac gtcatcgccg
     1381 acgatggccg gcagcgtgaa gagttctgcc tcgaccggcc cagcctgggc ctgtacctgc
     1441 cgcccatgac ctggggcatt cagcacaggt acacgcaggg cgccgtgctg ctggtgctgg
     1501 cgtcggaccc gtacgatccg gccgactaca tccgcgacta cgacgaattc ctggcattgc
     1561 gacgcgcggg aggcgctgcg tgatggcgcg cgaagccctg cgctacctgc tggcgggtgg
     1621 ggccaacacc gcgctgacct acctgctgta cctggcgctg ctgcgactgc tggactatcg
     1681 ctgggcatat gtgctggcgt tcctggctgg tgtcgcgctg tccttcgtgc tgttgcggca
     1741 tgtcgtgttc gcgcgcccgg gcaagcgctt ttcgtggatc tatgtgaccg ccagccacgg
     1801 cgtgcaactg ggcctgggcc tggtggtggt cgaggcctgg gtggcctggc tgcgcggtcc
     1861 ggcctggctg gcgccgctgg cggcggcgct ggtgtgtgtg ccggtcatct ttgttctgca
     1921 gcgctggatc ttcactccgc atgccgcgtc ctgaacacct gcgcccggcg ctgtcgaagt
     1981 ggtggctgcg tgccggcatg gcgctgccgc tgctgatgtt gggcgccaac ctgctggcct
     2041 ggctgcgctg gggcaccgac ctgccgtacc tggacgactg gcgcgtctac gacgacggca
     2101 gcgccctgtc gctcgatccg cggcatctgt ttcgcgccgt caacaacacc atctcgccgg
     2161 tcggcctgac cctggacgtg ctggcgcagc gctggctggg tggcaacccc ttgccctacc
     2221 aggcgctgtc catgctggcg gtgctgggtg ggttgctgtg gctgcagtgg cggctgttgg
     2281 gctgggcggt ccgggaccga cgccagcagg cattgctgtt cgccttctgt gttcccatgc
     2341 tgcaatcggg cagctactgg ggcgaacaga gcctggccta tcaccaggcc ttgcccttgc
     2401 tggcgctgct gggtgccatg gcgtgggcgt tgccacggcc gcggcgccgt gccgccctgg
     2461 gggtgttcgt cctgggtgcg gtggccggcc tgtcctacat ttccggcgcc atcgcggccc
     2521 tggtgctggg cgcgtgctgg ctggtgctgg cgcgggtgtt gcgcacgcac ccgacgccgg
     2581 cgctggccac ccgcatcgcc ggccccggtg gcgcgctgct gctggcgggc gggttgacca
     2641 gcgccttgca gttccatctg acgcgtggcg atgcgcaggc cagggatcgg cagtacatgg
     2701 gcctgactta ccccgattcg cccgacttct gggcattcct ggcgggcaag atcgggcggg
     2761 ccaccgggca tggtttcgcg tcgctcccgc tggagctgat ctgggtcgcg ctggtgctgc
     2821 tggccctgct ggcggcgctg ggcctgacag ggcgcgcagc gctggcgccc ggccgcgtgc
     2881 cgagcgttcg gcggcgtcgc gtcgccgtgg tgttcctgcc cttgtgcgca atcgtgctga
     2941 cctatctggt gctggtcagc ctgggccggg ccgggtttcg cgacccaggc atccagggcg
     3001 caggcggggt gttccggttc ggctacgagc gctttcactt cttctgggtc acgctgctgt
     3061 ttccgtgggt cgccgcggcc ctggttcccc ggcggcggac acgggcggtg ccggtgctgg
     3121 tcgggacatt gctgacggcg ctggcgctgg ccgcagtgcg cggtgtgttc gacgtgccat
     3181 cgttctaccg atcggcctcg gcgtaccgcg ccagtgagct tcgctgtctg gggcgtcagc
     3241 tcggcagcgg gcagcccatt cgctgccccg gctacgacat gatgggcatg accgacctga
     3301 cccgcgccta catccacgcg cgcgacatcc aggcctcttt cgtgcgttac ctgccggtgc
     3361 agacgcgcga cggttttggc cgtgagttgc tggactggca cgcggcggcg gggacggagc
     3421 ccgggccatg ggcccatgtc cggcgacggg ccgatggcgg gctgcagagc gagggcgacg
     3481 cccagctcac ggtcgcatgg cccgacgctg ctttgtcgcg acgctgccgc acgctgggtg
     3541 tgcagctgaa catcgtcacc gcgcacgccg aaaccgcgca gctgtactac cgatggtcgg
     3601 gccagcagag cgacagcgaa gcgcactccg tgcgccaggc ctatcagccg gacgtggatg
     3661 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgggcac ggtgcgcctg
     3781 tacggcgcgc ggggcgacgc cttcagctgg ggctcctggc tgctcaaggc ggggctgcca
     3841 cctcactgcg ccatcgagtc ggcgctcatg acgtaccact atggcctgtg gctgggattc
     3901 cactcggcct atttcgaagt cagacaggac aattccagcg tctggcggtt tcacgagaat
     3961 ttcggcgcga cccgcatcgg cgccgacgat cggcactttc attacgaact ggccgccgac
     4021 gcactgacgc aggccctgca gcgctaccgg cgctacctgc cgcacggcat acgcgtcagc
     4081 cgcgactgaa ccgactgcgc tgccgtcctg gagaacccct atgcacgtca tacccaccgc
     4141 actggccggc gtcctggtgc tcgaaccccg agtgttcggc gacgaccgtg gttttttcat
     4201 ggagagctac aaccgccgca gcttcgccca ggcgaccggc ctggacatcg acttcgtgca
     4261 ggacaaccac agccgcagcc gcaagggcgt gctgcgtggc ctgcactacc agctgcgcca
     4321 gccgcagggc aagctggtgc gggtgaccca cggtgccgtg ttcgacgtcg cggtggacat
     4381 ccgccgcggc tcacccacgt tcggccgctg ggtcggcgtc gagctcagtg ccgacaacca
     4441 ccgccagctt tgggtgccgc ccggcctggc gcacggcttc gtggtgctga gcgacagcgc
     4501 cgacttcctg tacaagacca ccgactacta cgcccccgag cacgagcgca gcatcgcctg
     4561 ggacgacccc gccatcggca tcgactggcc gctggccgcg catggcatca ccgcgccgct
     4621 gctgtcggcc aaggatcagg ccgggcagcc cctgtcgcgg gcggaactgg tggattgaaa
     4681 ggcagccccg ggatctctgg tgaacatcgc cgcctccatc gtcagtcacg gtcatggcgc
     4741 cccggtcgag gcgctgctgg cgcagctcgc ggcgctgggt gagcccgggc tggcgcgcgt
     4801 gatcgtcacc ttcaacgtcc ccgagccggc gtcggccgag cggttgcgtg ggcgccactg
     4861 gccgttccag ctgcacctcg tcgacaaccc ggcaccgctg ggttttgggg ccaaccacaa
     4921 ccgcgccttt gcgctcgacg cgcgcatcga tggcggccag ccgctgttcg cggtgctcaa
     4981 ccccgacctg cgctgcctgg tcaacccgtt tccgccgctg gtggccgagc tgcaaagcga
     5041 tccccgcgtc ggcgcggcct acccggtgca gaccgacgcg cgcggttgcc gtcaggacca
     5101 tgagcgcctg cgccccacgc cgctgcggct gctgcgccgg catctgctgg gtcggcgcat
     5161 ggaggtcggc gccggtcagg cgcccgactg ggtcaacgcc gccttcttgc tgctgcgccg
     5221 gccggccttc gaggccatcg ggggcttcga cgaggcgttt ca
//
LOCUS       DAOQTZ010000147         5196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_17615_length_5196_cov_14.237697, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000147 DAOQTZ010000000
VERSION     DAOQTZ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17615_length_5196_cov_14.237697"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(463..1665)
                     /locus_tag="PKN51_12600"
     CDS             complement(463..1665)
                     /locus_tag="PKN51_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT85982.1"
                     /translation="MRKTFLTPLVAALLAAGSLPARAADDVIRIGVLNDQSGLYSDFG
                     GSTSVIAARMAVEDFGGKVLGKPIEIVSADHQNKADVATSVARRWFDVDGVSAVADLT
                     NSAVALAVQQLAKGQGKVTLSSGPATTRLTNEDCSPTGFHWAFDTYSQAVGTAKAVVA
                     TGGKSWFILAADYAFGHQMAADLDRVVKESGGSVLGQVRAPTGTMDFSSFLLQAQSSK
                     AQIIGLANAGADTVNSIKQAGEFGIMSGGQKIAGLVVVISDVHALGLKNANGLLLTTA
                     FYWDRDDASRKWSKRFFDRTGRMPGMVQAGTYSSVLHYLKAMQAAGKTDGKAVADKMR
                     ELPVDDAFAKGKLRADGRLMHDMYLVEVKKPGESKGLWDYYKILRTIPAEDAAQPLSQ
                     SKCSLVKQ"
     gene            complement(1756..3018)
                     /locus_tag="PKN51_12605"
     CDS             complement(1756..3018)
                     /locus_tag="PKN51_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HNT85983.1"
                     /translation="MTSTASPSSSPERVFGWREHTALWFSLGVGLLVMQVGAYLVPAM
                     GTRDAALAVVGGSILGAGLLAWVAWLGCTSGQTSAGLIRTAYGQGFARLPILLNVVQL
                     LGWATFELVVMRDGTRAIARQALGIDPGLVAPTLLWGVIVLLLLRGSMLTLVRRIVSR
                     VGLPLVVLSLLWLSVQFGLQLQASGLDALWQRAGDGSMNGFQALDLVIAMPVSWLPLV
                     ADYARHGRSGRSALGGTWLGYTIANIWCYGLGVLVITVVGPGTDLVGALLLAQGGLIA
                     LGLILIDELDNTYGDLYSGSVSTHSLHGGLSLRSWGTALALVSIAFALVLPMHQLEPF
                     LLMLSSVFVPLFGTILGRLGTQPARLAEAPRGVHAAPAAIWLLGIVLYHLLAQWAPQF
                     GSALPTLAATFVLGALSARTGTRAMQPA"
     regulatory      complement(3073..3183)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3313..3624
                     /locus_tag="PKN51_12610"
     CDS             3313..3624
                     /locus_tag="PKN51_12610"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNT85984.1"
                     /translation="MKLTSSQLAPLLADLPGWRFDAQRGGLIEREYRFRDFAQAFGFM
                     AQVAVVAESRNHHPEWFNVYNQVKVALTTHDVDGLSMKDIELARFMDRAAAALTQEQA
                     P"
     gene            3621..4514
                     /gene="phhA"
                     /locus_tag="PKN51_12615"
     CDS             3621..4514
                     /gene="phhA"
                     /locus_tag="PKN51_12615"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HNT85985.1"
                     /translation="MNASHDDSRHGLADRSYRPERADWTIDQDWGRYSAAEHAVWKTL
                     FERQSRLLPGRACQAFVQGMRELPMRHDQIPRFDELSEALMKRTGWQVVAVPGLVPDE
                     VFFEHLAHRRFPAGQFIRRPDELDYLQEPDVFHDVFGHVPMLMHPVLAEFIQAYGQGG
                     LRAQKLGMLEQLARVYWYTVEFGLVLEQGAPRIYGAGIASSASESVFAVEGDSPNRIG
                     FELERVMRTRYRIDDFQESYFVLDNLDQLLQLAQIDFDPVYARLRSGPVYEPGEVLAS
                     DRVVSRGSGAYHAAKRAGGQP"
     gene            4511..>5196
                     /locus_tag="PKN51_12620"
     CDS             4511..>5196
                     /locus_tag="PKN51_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT85986.1"
                     /translation="MSGAQPAVLITGAGGALGGAVVAHFAQRGARLVLLERDGTALRR
                     QWGAGSGAQIVEADLLNREQVAAQLAGAGPIDVLCHLAGGFHMGEPVHATPAAAWDLM
                     MDLNARSLIHVAAAVVPGMLARGRGRIVTVGAAGALKGGASMGAYAASKSALMRLTES
                     MSAELRDAGINVNGVLPSTLDTPANRAAMPDADASRWVAPEALAEVIGFLASDAARAV
                     HGALIPVLGRV"
BASE COUNT          796 a         1798 c         1853 g          749 t
ORIGIN      
        1 ctggtcaact tcgggcagac cgcatcgatg cgggggctcg cggctgcgat ggcctgcacc
       61 tcctgggtga gcacgctgcg cacccacccg caccgcgctc ggcgcgccat gcggcgttgc
      121 tcctccttgc gggcaggagc ccgcgacatc gtcgcgtcgt gcctggcacg ccgaaagcgt
      181 tctcgaagag cggcgcagcc aaaatcggcc ccagcgagac cagtttttgc cggatttgca
      241 gcccaatagt cgagctattg ggcaaaaaga cggtgctctc gaagagcggc gaagccaaaa
      301 atgggccgct gcggtcgatt tgcagccggc gatttccaag tccgaccgcc tcctaggttg
      361 aacctgatcg gctgcagccc cggtacgtga cgctggccgg gcatggccgc gccgatcatg
      421 cgctgccgaa ggcgcgccgg cggcggccgg cgcgcccggg gttcactgct tcaccagcga
      481 gcacttgctc tggctgagcg gctgcgcggc atcttcggcg gggatggtgc gcaggatctt
      541 gtagtagtcc cacagcccct tggactcgcc cggcttcttg acctcgacca ggtacatgtc
      601 gtgcatcagc cggccgtcgg cacgcagctt gcccttggcg aaggcgtcgt ccaccggcag
      661 ttcgcgcatc ttgtcggcca ccgccttgcc gtcggtcttg ccggccgcct gcatggcctt
      721 caggtagtgc agcaccgacg aataggtgcc ggcctgcacc atgcccggca tgcggccggt
      781 gcggtcgaaa aagcgcttgg accacttgcg cgaggcgtcg tcgcggtccc agtagaacgc
      841 ggtggtcagc agcaggccgt tggcgttctt caggcccagc gcgtggacgt cgctgatcac
      901 caccaccagg ccggcgatct tctggccgcc cgacatgatg ccgaactcgc ccgcctgctt
      961 gatcgagttg acggtatcgg cccccgcgtt ggccaggccg atgatctgcg ccttggacga
     1021 ctgcgcctgc agcaggaagg aggagaaatc catcgtgccg gtgggggcgc gcacctggcc
     1081 gagcacgctg ccgccgcttt ccttgacgac gcggtccaga tcggccgcca tctggtgccc
     1141 gaacgcgtag tcggcggcca ggatgaacca gctcttgccg ccggtggcca ccaccgcctt
     1201 ggccgtgccc acggcctgcg agtaggtgtc gaacgcccag tgaaagccgg tgggcgagca
     1261 gtcctcgttg gtcaggcggg tggtggccgg cccggacgac agggtcacct tgccctgccc
     1321 cttggccagc tgctgcaccg ccagcgccac ggccgaattg gtcaggtcgg cgacggcgct
     1381 gacgccgtcc acgtcaaacc agcggcgcgc cacggacgtg gcgacgtcgg ccttgttctg
     1441 gtggtcggcc gagacgatct cgatcggctt gcccagcacc ttgccgccaa agtcttccac
     1501 cgccatgcgg gctgcgatca ccgaggtgga gccgccgaag tcggaataca ggcccgactg
     1561 gtcgttcagc acgccgatgc ggatcacgtc gtcggcggcc cgggcgggca ggctgcccgc
     1621 ggccagcagg gccgcgacca ggggagtcag aaacgtcttg cgcatgcacc ctctccttgt
     1681 gttgaaagcg ccgatcacta tagggcgggg gccggcgcgg cgacaccggg gaatcccccg
     1741 atccggcgta ctggctcagg ccggctgcat cgcccgcgta ccggtgcgcg cgctcagcgc
     1801 gcccagcacg aaggtggccg ccagcgtggg cagcgcgctg ccgaactgtg gcgcccactg
     1861 cgccagcagg tggtagagca cgatgccgag cagccagatc gccgccggcg cggcgtgcac
     1921 cccgcgcggg gcctcggcca ggcgcgccgg ctgggtgccc aggcgcccca ggatggtgcc
     1981 gaacagcggc acgaacaccg agctgagcat gagcaggaag ggctccagct ggtgcatcgg
     2041 cagcaccagt gcgaaggcga tcgaaaccag cgccagcgcc gtgccccagc tgcgcaggct
     2101 caggccgccg tgcaggctgt gggtcgagac cgagcctgaa tacaggtcgc cgtaggtgtt
     2161 gtcgagctcg tcgatcagga tcagcccgag cgcgatcagg ccgccctgcg ccagcagcag
     2221 ggcgccgacc aggtcggtgc cgggaccgac gacggtgatc accagcacgc ccaagccgta
     2281 gcaccagatg ttggcgatgg tgtagcccag ccaggtgccg cccagggcgc ttcggccgct
     2341 ccggccgtgg cgcgcgtagt cggccaccag cggcagccac gacaccggca tggcgatcac
     2401 caggtccagc gcctgaaagc cgttcatgct gccgtcgccg gcgcgctgcc acagggcgtc
     2461 caggccgctg gcctgaagct ggaggccgaa ctgcacgctg agccagagca gcgacagcac
     2521 caccagcggc aggccgacgc ggctgacgat gcggcgcacc agcgtcagca tcgagccgcg
     2581 cagcagcagc agcacgatca cgccccacag cagcgtgggc gccaccaggc ccgggtcgat
     2641 gcccagcgcc tgccgggcga tggcccgcgt accgtcgcgc atcaccacca gctcgaaggt
     2701 ggcccagccc agcaactgca ccacgttgag caggatcggc aggcgcgcaa agccctggcc
     2761 gtaggcggtc cggatcaggc cggcgctggt ctggccgctg gtgcagccca gccaggccac
     2821 ccaggccagc agcccggcgc ccaggatgga gccgcccacc accgccagcg ccgcatcgcg
     2881 cgtgcccatg gccggcacca ggtaggcgcc cacctgcatc accagcaggc cgacgcccag
     2941 gctgaaccac agggcggtgt gctcgcgcca gccgaacacg cgctcgggcg aggaagaggg
     3001 ggaagcagtg gatgtcatgg tgcgccggtc cgtcaaaaaa atgcaggagg caggcgcgcg
     3061 gcggcccggc ggccgcccgg cgagctgctt tcctgcgcga ggatgatctc aatcaggttc
     3121 gaagggactg tctcagccgc accgcgccgt cgaaacggcc gcgcggcacc cccagcgatg
     3181 ctcgaagggg gtcgattctg ccgcagcggg gccggtgctg tccatgaccc tcgcggcgac
     3241 gggcatgctg ccggcggcgc catcggtcga cccgtggcgt ggctatcatc gatcgactgg
     3301 agggacgtgc ccatgaaact cacctcgtca caactggcgc ccctgctggc cgacctgccc
     3361 ggctggcgct tcgatgcgca gcgtggcggc ctgatcgagc gcgagtaccg gttccgcgac
     3421 ttcgcccagg ccttcggctt catggcgcag gtggcggtgg tggccgaaag ccgcaaccac
     3481 cacccggaat ggttcaacgt ctacaaccag gtcaaggtgg cgctgaccac ccacgacgtg
     3541 gacggcctgt cgatgaagga catcgaactg gcgcgcttca tggatcgggc ggccgcggcc
     3601 ctgacgcagg agcaggcgcc atgaacgcgt cgcacgacga cagccggcac ggcctggccg
     3661 accgcagcta ccggcccgag cgggccgact ggaccatcga ccaggactgg ggccgctaca
     3721 gcgccgccga gcacgcggtc tggaagacgc tgttcgagcg ccagagccgc ctgctgcccg
     3781 gccgcgcctg ccaggccttc gtgcagggca tgcgcgagct gccgatgcgc cacgaccaga
     3841 tcccgcgctt cgacgagctg tccgaggcgc tgatgaagcg caccggctgg caggtggtgg
     3901 cggtgcccgg cctggtgccg gacgaggtgt ttttcgaaca tctggcgcat cggcgctttc
     3961 cggccggcca gttcatccgt cggccggacg agctcgatta cctgcaggag cccgacgtgt
     4021 tccatgacgt gttcggccat gtgccgatgc tgatgcatcc ggtgctggcc gagttcatcc
     4081 aggcctatgg ccagggcggg ctgcgcgccc agaagctggg catgctggag cagctggcgc
     4141 gggtgtactg gtacacggtg gaattcggcc tggtgctgga gcagggcgcg ccgcgcattt
     4201 atggcgccgg cattgcgtcg tcggcgtccg agtcggtgtt tgcggtggag ggcgattcgc
     4261 ccaaccgcat cggcttcgag ctcgagcgcg tgatgcgcac acgctaccgc atcgacgatt
     4321 ttcaggagag ctatttcgtg ctggacaacc tggatcagct gctgcagctg gcgcagatcg
     4381 acttcgaccc ggtgtatgcg cggctgcgca gtggcccggt gtatgagccc ggcgaggtgc
     4441 tggccagcga ccgcgtggtc agccgcggca gcggcgccta ccacgccgcc aaacgcgccg
     4501 ggggccagcc atgagcggcg cgcagcctgc ggtgttgatc accggcgccg gcggcgcgct
     4561 gggcggcgcg gtggtggcgc attttgccca gcgcggcgcg cgcctggtgc tgctggagcg
     4621 cgatggcacg gccctgcgtc gccagtgggg cgcgggcagc ggcgcgcaga tcgtcgaggc
     4681 cgacctgctg aaccgcgagc aggtggcggc gcaactggcc ggggcgggcc cgatcgatgt
     4741 gctgtgccat ctggccggcg gctttcacat gggcgagccg gtgcacgcca cgcccgctgc
     4801 ggcgtgggat ttgatgatgg acctgaacgc gcgcagcctg atccacgtgg ccgccgccgt
     4861 ggtgccgggt atgctggcgc gcggccgtgg gcgcatcgtc accgtgggcg cggccggcgc
     4921 gctgaagggc ggcgccagca tgggcgccta tgccgcgtcc aagagcgcgc tgatgcggct
     4981 gaccgaatcc atgtcggccg agctgcgcga cgccggcatc aacgtcaacg gcgtgctgcc
     5041 ctccaccctg gacacgcccg ccaaccgcgc cgccatgccc gacgccgacg ccagccgctg
     5101 ggtcgcaccc gaggcgctgg ccgaggtgat cggctttctg gcctcggacg cggcgcgcgc
     5161 cgtgcatggg gcgctgattc cggtgctggg gcgggt
//
LOCUS       DAOQTZ010000148         5159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_17794_length_5159_cov_13.368730, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000148 DAOQTZ010000000
VERSION     DAOQTZ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17794_length_5159_cov_13.368730"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..851
                     /locus_tag="PKN51_12625"
     CDS             237..851
                     /locus_tag="PKN51_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNT85987.1"
                     /translation="MRSLLLHRRAKPLVFVLCLLPLAWLSYGAAADQLGANPAEALIR
                     GLGDWALRFLCLALAVTPLRIRTRTPQLARFRRLLGLFTFFYACLHLLAYGWLDMGLD
                     PGDIGRDILKRPFILVGFASWLLLLPLALTSTHRAVRALGGRQWQALHRLVYVVAPLV
                     LLHFFWMRAGKNDFAEVAVYALILGALLLERLLRYFEPKSRLSA"
     gene            complement(933..2228)
                     /gene="lysA"
                     /locus_tag="PKN51_12630"
     CDS             complement(933..2228)
                     /gene="lysA"
                     /locus_tag="PKN51_12630"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNT85988.1"
                     /translation="MTSPATTTPALLPGGAALSFQNGQLMLEQVALSALAREHGTPLF
                     VYSRAAILSALAAYQRGFAGRRAQICYAMKANASLAILRLLAEAGCGFDIVSVGELER
                     ALAAGATPSKIVFSGIGKTRQEMRRALEVGIGCFNVESLPELDVLSEVAQSCGQRAPV
                     SIRVNPNVDARTHPYISTGLKGNKFGIAHEQALAAYRHAASLPGLRVAGIDCHIGSQI
                     TEVAPYLEAMDRVLDLVTAIEAAGIAIGHIDFGGGLGIDYRGETPPAADALWAELLAR
                     MDARGFGERQIMIEPGRSLVGNAGVCLTEVLYLKPGEQKNFCIVDAAMNDLPRPALYE
                     AYHRIVPVVQATPDAATSTWDVVGPVCESGDWLGRERALAVRPGDHLAVLSAGAYCMS
                     MASNYNARPRAAEVLVDGAQARLIRRREQLQDLWRDELG"
     gene            2487..2819
                     /gene="cyaY"
                     /locus_tag="PKN51_12635"
     CDS             2487..2819
                     /gene="cyaY"
                     /locus_tag="PKN51_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HNT85989.1"
                     /translation="MTDLEYLDRAETLLRQVEAQCDHLNDHSDADIDNQRSGGMLTLV
                     FPDRSQIVVNLQKPLHEVWLAARGGGYHFRFVDGAWRDTKSGQEFFRQLSQSASEHAG
                     LALRFAPD"
     gene            complement(2862..>5159)
                     /locus_tag="PKN51_12640"
     CDS             complement(2862..>5159)
                     /locus_tag="PKN51_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HNT85990.1"
                     /translation="LAGVLSVLLGGLMALAIAYPNLPDVSELSNYRPKLPLRVYTADG
                     TLMREFGEERRNLTPIGEIPQVMKHAVLAIEDARFYEHSGVDYLGVARAALANLGREK
                     SQGASTITMQVARNVYLSSEKTYTRKIYEVLLTFKLEHLLSKDQILEIYMNQIFLGNR
                     AYGFAAASETYFGKPLKDVSIAEAAMLAGIPKFPSTANPLANFTRARDRQLHIIDRMQ
                     DNGFITAEQAAAAKQQELRIRPVNEASRVHAEYVAEMVRQMMFAQYGDDTYSRGLNVY
                     TSIRAADQNAAYTALRAGILDYDRRQAYRGPEKFIELPGNPKQLDEAVDDALASHPDA
                     GELLAAVVTRVDAQGRSASVMRRGGETVEIAADGLRAVASGLSAKAGPNIRIRPGAVV
                     RIVRNSRNQWAMTQIPEVEGALVALDPRNGAIKALVGGFDFDKNKFNHVTQAWRQPGS
                     SFKPFIYSAALEKGFSPTTMINDTPLFFDASVTGGQPWEPKNYEGGFEGPMTMKRALA
                     RSRNIVSIRILQTITPRYGQEWITRFGFDADKHPPYLTMALGAGSVTPIQLASGFAVF
                     ANGGYRISPFLITRVTDHTGKVLVESQAEPPSESSRAIDARNAFIMNSMLQEVARSGT
                     AAKAQATLKRPDIGGKTGTTNDSMDAWFTGYQPTLVTTVWVGYDTPRNLGSRETGGGL
                     SLPIWINFMQTALKGVPVADAKPPEGVVQVGGDWYYDEYARGGGISNLGGAGLEAADP
                     AAGQEAGAGAASSGERNRILDLFRN"
BASE COUNT          730 a         1748 c         1808 g          873 t
ORIGIN      
        1 gcacaccgat cacggcactg gcaggcgcat ccaactgccg tttctaggtt gaaccccgac
       61 agaccgcggt cgaccgcggg ctccccgcat gaaccccgca tgggcaccgc gtctcggtcc
      121 aggatcgatc ttccggcctt cgggtggggt gacgccgtgc tgccggatgc tataaacaac
      181 atagctgctg gcgattgtca gacaggcgcc gcagcccgat ttgacctata ttcctgatgc
      241 gttccctgtt gctgcatcgg cgggccaagc cgctggtttt cgtgctgtgc ctgctgccgc
      301 tggcctggct gagctatggc gccgccgccg accagctggg cgccaacccg gccgaggcgc
      361 tgatccgcgg gctgggcgac tgggcgctgc gctttctgtg tctggcgctg gcggtgacgc
      421 cgctgcgcat ccgcacccgt acgccgcagc tggcgcgctt tcggcgcctg ctggggctgt
      481 tcaccttctt ctacgcctgc ctgcacctgc tggcctacgg ctggctcgac atggggctgg
      541 atccgggcga catcgggcgc gacatcctca agcgcccctt catcctggtc ggcttcgcct
      601 cctggctgct gctgctgccg ctggcgctga cgtccaccca ccgcgccgtg cgtgccctgg
      661 gcgggcgcca gtggcaggca ctgcaccggc tggtgtacgt ggtggcaccg ctggtgctgc
      721 tgcacttctt ctggatgcgc gcgggcaaga acgacttcgc cgaagtggcg gtctacgccc
      781 tcatcctggg cgccctgctg ctggagcgcc tgctgcgcta tttcgagccc aaatcaaggc
      841 tcagcgcatg atgggcaagc gccggcagct ctcatattga tagtcaccgg aacggtgggc
      901 gacgcccgcc gggcggcgcc gccggcaggc gctcagccga gttcgtcgcg ccacaggtcc
      961 tgcaactgtt cgcggcggcg gatcaggcgc gcctgggcgc cgtcgaccag cacctcggcg
     1021 gcgcgcgggc gggcgttgta gttgctggcc atgctcatgc agtaggcgcc ggccgacagc
     1081 acggccagat ggtcgcccgg tcgcaccgcc agtgcgcgct cgcgccccag ccagtcgccg
     1141 ctttcacaca ccgggccgac cacgtcccag gtgctggtgg cggcatccgg ggtcgcctgc
     1201 accaccggca cgatgcggtg atacgcctcg tacagcgccg gccgcggcag gtcgttcatg
     1261 gcggcatcga cgatgcagaa gttcttctgc tcacccggct tgaggtacag cacctcggtc
     1321 aggcacacgc cggcgttgcc caccagcgag cggccgggct cgatcatgat ctggcgctcg
     1381 ccgaagccgc gcgcgtccat gcgcgccagc agctcggccc acagcgcgtc ggccgccggc
     1441 ggcgtttcgc cccggtagtc gatgcccagg ccgccgccga agtcgatgtg gccgatggcg
     1501 atgccggccg cctcgatggc cgtcaccagg tccagcacgc ggtccatggc ttccaggtag
     1561 ggtgccactt cggtgatctg cgagccgatg tggcagtcga tgccggccac gcgcaggccg
     1621 ggcaggctgg cggcgtgccg gtaggcggcc agcgcctgct cgtgcgcaat gccgaacttg
     1681 ttgcccttca ggccggtgga gatgtagggg tgggtgcgcg catccacgtt ggggttgacg
     1741 cggatgctga ccggcgcgcg ctggccgcag gactgggcca cttcgctcag cacgtccagc
     1801 tcgggcaggc tctcgacgtt gaagcagccg atgcccactt ccagcgcgcg gcgcatttcc
     1861 tggcgcgtct tgccgatgcc cgaaaacacg atcttcgagg gcgtggcgcc ggccgccagg
     1921 gcacgttcga gctcgcccac cgagacgatg tcgaagccgc agccggcctc ggccagcagg
     1981 cgcaggatgg ccagcgaggc gttggccttc atggcatagc agatctgcgc gcggcggccg
     2041 gcaaagccgc gctggtaggc ggccagcgcc gacaggatgg cggccctgga atagacgaac
     2101 agcggcgtgc cgtgctcgcg cgccagcgcg gacagcgcga cctgttcgag catcagctgg
     2161 ccgttctgga aggacagcgc ggcgccgccg ggcagcagcg ccggcgtggt ggtggcggga
     2221 gaggtcattg gatgtcgggt aagtccggca gaaccggggg tgggggggag cggctgggcg
     2281 ctgcggcagg cgccggcgcg gcggtggatt ttgcaccacc gaacaccgtc tgtggcaggc
     2341 tggcacgctg cgccgccgcc gggctgtcgg gcaggtacaa cgcccctttc tggccgcagc
     2401 cgccggccag ggccgccacg gccagggcca tgccgctgac tagaatgaac cgacgcggct
     2461 gtgcaaacga catggatgcc attgtaatga ccgacctcga atacctggac cgcgccgaga
     2521 ccctgctgcg ccaggtcgag gcgcagtgcg accacctcaa cgaccacagc gacgccgaca
     2581 tcgacaacca gcgcagcggc ggcatgctga ccctggtgtt tcccgaccgc agccagatcg
     2641 tcgtcaacct gcagaaaccc ctgcacgagg tgtggctggc ggcgcgcggg ggcggctacc
     2701 actttcgctt cgtcgacggt gcctggcgcg acacgaagtc aggacaggag ttctttcgcc
     2761 agttgtcgca aagcgccagc gaacacgccg gactggcgct gcgcttcgcg cccgactgac
     2821 cgcatcggaa ccacgagggc gccgggcgcc ctcacaggcg ctcagtttct gaacagatcg
     2881 aggatgcggt tgcgttcacc cgatgaggct gcgcccgcgc ccgcttcctg cccggctgcc
     2941 gggtcggccg cttccaggcc ggcaccgccc aggttgctga tgccgccacc gcgcgcgtac
     3001 tcgtcgtagt accagtcgcc gcccacctgc accacgccct cgggcggctt ggcgtcggcc
     3061 accggcaccc ccttgagcgc ggtctgcatg aaattgatcc agatcggcag cgacaggccg
     3121 ccgccggttt cgcggctgcc caggttgcgc ggcgtgtcgt agcctaccca caccgtggtc
     3181 accagggtcg gctggtagcc ggtgaaccag gcgtccatgg aatcgttggt ggtgccggtc
     3241 ttgccgccga tgtcgggccg cttcagggtc gcctgcgcct tggcggcggt gccgctgcgc
     3301 gccacctcct gcagcatgct gttcatgatg aaggcattgc gcgcgtcgat ggcgcggctg
     3361 gactcgctcg gcggttcggc ctggctttcc accagcacct tgcctgtgtg gtcggtgacg
     3421 cgggtgatca ggaacgggct gatgcggtag ccgccgttgg cgaacacggc aaaacccgag
     3481 gccagttgga ttggcgtcac cgaaccggcc cccagcgcca tggtcaggta gggcgggtgc
     3541 ttgtcggcgt cgaagccgaa gcgcgtgatc cattcctgcc cgtagcgcgg cgtgatggtc
     3601 tgcaggatgc ggatcgacac gatgttgcgc gagcgcgcca gggcacgctt catggtcatc
     3661 gggccctcga agccgccctc gtagttcttg ggctcccagg gctggccacc ggtcacgctg
     3721 gcgtcgaaga acagaggcgt gtcgttgatc atggtggtgg gcgaaaagcc cttttccagc
     3781 gccgccgaat agatgaaggg cttgaagctg gagcccggct ggcgccacgc ctgggtgacg
     3841 tggttgaact tgttcttgtc gaaatcgaac ccgcccacca gcgccttgat ggcgccgttg
     3901 cgcggatcca gcgccaccag ggcgccttcc acttcgggaa tctgggtcat ggcccactgg
     3961 ttgcggctgt tgcgcacgat gcgcaccacc gcgcccgggc ggatgcggat gttcggcccg
     4021 gccttggccg acagtcccga agccaccgcg cgcaggccgt cggcagcgat ctccaccgtc
     4081 tcgccgccgc ggcgcatgac gctggcgctg cgcccctgcg catccacgcg tgtgaccacg
     4141 gcggccagca gctcgccggc gtcggggtgg ctggccagcg cgtcgtccac cgcctcgtcg
     4201 agctgcttcg ggttgccggg cagctcgatg aacttctccg ggccgcggta ggcctggcgg
     4261 cggtcgtagt ccaggatgcc ggcgcgcagc gcggtgtagg cggcgttctg gtcggcggcg
     4321 cggatcgagg tgtagacgtt caggccgcgg ctgtaggtgt cgtcgccgta ctgcgcgaac
     4381 atcatctggc gcaccatctc ggccacgtac tccgcgtgca cgcgactggc ctcattgacg
     4441 ggccggatgc gcagctcctg ctgcttggcg gccgccgcct gctcggccgt gatgaagccg
     4501 ttgtcctgca tgcggtcgat gatgtgcagc tgccggtcgc gcgcgcgcgt gaagttggcg
     4561 agcgggttgg cggtggacgg gaacttcggg atgccggcca gcatggcggc ttcggcgatg
     4621 ctgacgtcct tgagcggctt gccgaaatac gtctccgacg cggcggcgaa cccgtaggcg
     4681 cggttgccca ggaagatctg gttcatgtag atctccagga tctggtcctt gctcagcagg
     4741 tgttccagct tgaaggtcag cagcacctcg tagatcttgc gggtgtaggt tttctcggac
     4801 gacaggtaga cgttgcgcgc cacctgcatg gtgatggtgg aggcgccctg ggacttctcg
     4861 cgccccaggt tggccagggc ggcgcgcgcc acgcccaggt aatcgacccc gctgtgttca
     4921 taaaagcgcg cgtcctcgat cgccagcacg gcgtgcttca tgacctgcgg aatctcgccg
     4981 atcggggtca ggttgcggcg ttcttcgccg aactcacgca tcagggtgcc gtcggccgtg
     5041 tacacgcgca gcggcagctt gggccggtag ttcgacagct cggacacgtc cggcaggttc
     5101 gggtaggcga tggccagcgc catcagcccc cccagcagca ccgacagcac cccggccag
//
LOCUS       DAOQTZ010000149         5068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_18242_length_5068_cov_13.638939, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000149 DAOQTZ010000000
VERSION     DAOQTZ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18242_length_5068_cov_13.638939"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKN51_12645"
     CDS             <1..274
                     /locus_tag="PKN51_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT85991.1"
                     /translation="QMPSWESVLRAADIMSVNRVSIDIGPTRHGITQGTTIYFFDPSG
                     NRLETFCGGYDHYPDMHPVTWSWDEVGRGIFYHDRKLNDAFLSVVT"
     gene            284..718
                     /locus_tag="PKN51_12650"
     CDS             284..718
                     /locus_tag="PKN51_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNT85992.1"
                     /translation="MARHSVEQRAITAEAAHAAVAAAVAKAGELGIRINVAVTDASGV
                     LAAFLRMPGAFLHSVDIAIDKAYTAAGFGFATGQWAGILRGDEALRLGMPVRPRNVVF
                     GGGLPMREQGVLIGGIGVSGGSAEQDEICARAGLAALGLEAA"
     gene            750..2210
                     /locus_tag="PKN51_12655"
     CDS             750..2210
                     /locus_tag="PKN51_12655"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HNT85993.1"
                     /translation="MKEFLNFINGQYVKSASGSTFENRNPVNGSLIGMIHEAGKPEVD
                     AAVAAAKAALTGDWGRISVTERCKLLDAVALEINRRFDDFLQAEIADTGKPHHLASHV
                     DIPRGAANFQIFIDTIKSASTESFNMTTPDGRTALSYGVRVPRGVIAVICPWNLPLLL
                     MTWKVGPALACGNTVVVKPSEETPATATLLGEVMNAVGVPKGVYNVVHGFGPGSAGEF
                     LTRHPDVNGITFTGETRTGEAIMKAAAEGIRPVSMELGGKNAAVVFADCDLKVTIDTL
                     QRSCFENAGQVCLGTERVYVERPIYEQVVAALKERAEAMTPGEPFDPATRIGPVISKE
                     HQAKVLSYYQKAREQGATVVTGGGVPDMPPHLKDGSWVQPTIWTGLPETASVITDEIF
                     GPCCHISPFDSEEEVLGKVNANRYGLATAIFTQDIGRANRVAQRIHVGLTWINCWFLR
                     DLRTSFGGSKASGIGREGGIHSFEFYTDLRNVMVKF"
     gene            2277..3059
                     /gene="dmpE"
                     /locus_tag="PKN51_12660"
     CDS             2277..3059
                     /gene="dmpE"
                     /locus_tag="PKN51_12660"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxopent-4-enoate hydratase"
                     /protein_id="HNT85994.1"
                     /translation="MDKSQIEQLGDRLFDALTQCTSVPPLTSQHPDITVDDAYAIQQR
                     LMQRRLAAGERVVGKKIGVTSQAVMNMLGVFQPDFGWLTDGMVYNEGEAVPMSRLIQP
                     KAEGEIAFVLKKTLQGPGISVADVLAATEGVMACFEIVDSRIQDWKIKIQDTVADNAS
                     CGVFVLGDRLVDVRDVDLTTCGMVLEKNGEIVATGAGAAALGHPANAVAWLANTLGRH
                     GIALEAGEVVLSGSLGIMVPVAAGDNLRVTIGGIGGCSVRFV"
     gene            3177..4091
                     /locus_tag="PKN51_12665"
     CDS             3177..4091
                     /locus_tag="PKN51_12665"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HNT85995.1"
                     /translation="MSKKIKCALIGPGNIGTDLLAKLQRSPVLEPVWMVGIDPDSDGL
                     KRAREMGIKTTDQGVDGLVPHIRKDGVQIVFDATSAYVHAENSRKVTEQGAMMVDLTP
                     AAIGPYCVPPVNLREHVGKREMNVNMVTCGGQATIPMVAAVSRVQPVAYAEIVAAVSS
                     KSAGPGTRKNIDEFTRTTAGAIEKVGGARKGKAIIIINPAEPPLIMRDTVHCLVEGQP
                     DQAAITKSIHDMMAEVQKYVPGYKLVNGPVFDGQRVSVFLEVEGLGDYLPRYAGNLDI
                     MTAAAARTAEMFAEEILAGSLTLEPVDV"
     gene            4105..>5068
                     /gene="dmpG"
                     /locus_tag="PKN51_12670"
     CDS             4105..>5068
                     /gene="dmpG"
                     /locus_tag="PKN51_12670"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HNT85996.1"
                     /translation="MSIQGKKITLHDMTLRDGMHPKQHQMTLAQMKSIAQGLDAAGMP
                     LIEVTHGDGLGGASVNYGFPAHSDEEYLSAVIPLMKQAKVSALLLPGIGTVDHLKMAR
                     ELGVHTIRVATHCTEADVSEQHITMARKMDMDTVGFLMMAHMNSAEGLVKQARLMEGY
                     GANCIYVTDSAGYLLPDQVKERIAAVRAALKPETELGFHGHHNLAMGVANSIAAIEAG
                     ANRIDAAAAGLGAGAGNTPMEVLVAVLERMGVHTGVDVFKIQDVAEDLVVPVMDFPIR
                     IDRDALTLGYAGVYGSFLLFAKRAEKKYGVPAREILVEMGRRGMV"
BASE COUNT          925 a         1709 c         1679 g          755 t
ORIGIN      
        1 ccagatgccg agctgggaaa gcgtgctgcg cgccgccgac atcatgagcg tcaaccgcgt
       61 ctcgatcgac atcggtccca cgcgccacgg catcactcag ggcacgacga tctacttctt
      121 cgaccccagc ggcaaccgcc tggaaacctt ctgcggcggc tacgaccatt accccgacat
      181 gcatccggtc acctggagct gggatgaggt cgggcgcggc atcttctacc acgaccgcaa
      241 gctgaacgac gccttcctga gcgtggtgac ctgaggcgcg ccgatggcac gacacagcgt
      301 cgaacaacgc gccatcaccg ccgaggcggc gcacgccgcc gtggcggcgg cggtggccaa
      361 ggccggcgag ctgggcattc gcatcaacgt cgcggtgacc gacgcctcgg gcgtgctggc
      421 ggcctttctg cgcatgcccg gcgcctttct gcactcggtg gacatcgcca tcgacaaggc
      481 ctacaccgcg gccggcttcg gttttgccac cgggcagtgg gccggtatct tgcgcggcga
      541 cgaggcgctg cgcctgggca tgccggtgcg gccgcgcaac gtggtgttcg gcggcgggtt
      601 gccgatgcgc gagcaagggg tgctgatcgg cggcattggc gtgtccggcg gatcggccga
      661 acaggacgag atctgcgccc gcgcgggcct ggccgcgctg gggctggagg ccgcgtgagc
      721 ggccgagctt ccaggaacag agagcaccca tgaaagagtt tttgaatttc atcaacggcc
      781 agtacgtcaa gagcgccagc ggcagcacgt tcgagaaccg caacccggtc aacggcagcc
      841 tgatcggcat gatccatgaa gccggcaagc ccgaagtgga cgccgccgtg gcggccgcca
      901 aggccgcgct cacgggcgac tggggccgca tttcggtcac cgagcgctgc aagctgctgg
      961 atgccgtggc gctcgagatc aaccggcgct tcgacgactt cctgcaggcc gagatcgccg
     1021 acaccggcaa gccgcaccac ctggcctcgc acgtggacat cccgcgcggc gcggccaact
     1081 ttcagatctt catcgacacc atcaagagcg cctccaccga gtcgttcaac atgaccacgc
     1141 ccgacggcag gaccgcgctc agctacggcg tgcgcgtgcc gcgcggcgtg atcgcggtga
     1201 tctgcccctg gaacctgcca ctgctgctga tgacctggaa ggtcggcccg gcgctggcct
     1261 gcggcaacac ggtggtggtc aaaccgtccg aagaaacccc cgccaccgcc accctgctgg
     1321 gcgaggtgat gaacgccgtg ggtgtgccca agggcgtgta caacgtggtg cacggcttcg
     1381 gccccggttc ggccggcgaa ttcctgaccc ggcaccccga cgtcaacggc atcaccttca
     1441 ccggcgagac gcgcaccggc gaagccatca tgaaggccgc cgccgagggc atccgcccgg
     1501 tgtcgatgga gctgggcggc aagaacgccg ccgtggtgtt cgccgattgc gatctgaagg
     1561 tcaccatcga cacgctgcag cgctcctgct tcgaaaacgc cggccaggtc tgcctgggca
     1621 ccgagcgcgt ctatgtggag cgaccgatct acgagcaggt ggtggcggcg ctgaaggagc
     1681 gcgccgaggc catgaccccc ggcgagccgt tcgacccggc gacacggatc ggcccggtca
     1741 tcagcaagga gcatcaggcc aaggtgctgt cctactacca gaaggcgcgc gaacagggcg
     1801 ccaccgtggt gaccggcggc ggcgtgcccg acatgccgcc gcacctgaag gacggcagct
     1861 gggtgcaacc gaccatctgg accggcctgc ccgagaccgc cagcgtcatc accgacgaga
     1921 tcttcggccc ctgctgccac atcagcccct tcgacagcga agaggaggtg ctgggcaagg
     1981 tcaacgccaa ccgctacgga ctggcgacgg cgatcttcac gcaggacatc ggccgcgcca
     2041 accgggtggc gcagcgcatc cacgtcggcc tcacctggat caactgctgg tttctgcgcg
     2101 acctgcggac ctcgtttggc ggctccaagg cctcgggcat cggccgcgaa ggtggcatac
     2161 actcgttcga gttttacacc gatctgcgca acgtgatggt caagttctga gcgatatagg
     2221 ccgctcgagc ttgactggca agcgcgagca gctctcattt tgatagtatt gaaaccatgg
     2281 acaagtcaca gatcgagcag ttgggcgacc gcctttttga cgccctcacg cagtgcacca
     2341 gcgtgccgcc gctgaccagc cagcaccccg acatcacggt ggacgatgcc tacgccatcc
     2401 agcagcggct gatgcagcgc cgcctggccg ccggcgagcg ggtggtgggc aagaagatcg
     2461 gcgtgaccag ccaggcggtg atgaacatgc tgggcgtgtt ccagcccgac ttcggctggc
     2521 tgaccgacgg catggtctac aacgagggcg aggccgtgcc catgagccgg ctgatccagc
     2581 ccaaggccga gggcgagatc gccttcgtgc tcaagaagac cctgcagggc cccggcatca
     2641 gcgtggccga cgtgctggcc gccaccgaag gcgtgatggc ctgcttcgaa atcgtcgact
     2701 cccgcatcca ggactggaag atcaagattc aggacacggt ggccgacaac gcctcctgcg
     2761 gcgtgtttgt gctgggcgat cgcctggtcg atgtgcgcga cgtcgatctg accacctgcg
     2821 gcatggtgct ggaaaagaac ggcgaaatcg tcgccaccgg tgccggcgcc gccgccctgg
     2881 gccacccggc caacgcggtg gcctggctgg ccaacaccct gggccgccat ggcattgccc
     2941 tggaggcggg cgaggtggtg ctgtcgggct cgctgggcat catggtgcca gtggccgcgg
     3001 gcgacaacct gcgcgtcacg atcggcggca tcggcggctg cagcgtgcgg tttgtttgaa
     3061 cgacgaatga ccttgcttcg cgtccatgac ggcgctgtac gccctgaccg aggccgtcat
     3121 ttcgtctttg aagccgcgcg agcggtgaag tcatttcatc attggaaagc atggaaatga
     3181 gcaagaaaat caagtgtgcc ctgatcggcc caggcaacat cggcaccgat ctgctggcca
     3241 agctgcagcg cagcccggtg ctggagccgg tgtggatggt gggcatcgac cccgactccg
     3301 acggtctcaa gcgcgcgcgc gagatgggca tcaaaaccac cgaccagggg gtcgatggtc
     3361 tggtgccgca catcaggaag gacggcgtgc agatcgtgtt cgacgccacc agcgcctacg
     3421 tgcacgctga aaactcgcgc aaggtcaccg agcagggcgc gatgatggtg gacctgacgc
     3481 ccgccgccat cggtccctac tgcgtgccgc cggtcaacct gcgcgagcac gtgggcaagc
     3541 gcgaaatgaa cgtcaacatg gtcacctgcg gcggccaggc caccatcccc atggtggccg
     3601 ccgtcagtcg cgtgcagccg gtggcctatg ccgaaatcgt ggccgccgtg tccagcaagt
     3661 ccgccggccc gggcacgcgc aagaacatcg acgaattcac ccgcaccacg gcgggcgcga
     3721 tcgaaaaggt gggcggcgcc cgcaagggca aggccatcat catcatcaac ccggccgagc
     3781 cgccgctgat catgcgcgac accgtgcact gcctggtcga gggccagccc gatcaggccg
     3841 ccatcaccaa gagcatccac gacatgatgg ccgaggtgca gaagtatgtg cccggctaca
     3901 agctggtcaa cggcccggtg ttcgacggcc agcgggtgtc ggtgttcctg gaggtcgagg
     3961 gcctgggcga ctacctgccc aggtacgccg gcaacctcga catcatgacc gccgccgccg
     4021 cgcgcaccgc cgagatgttt gccgaagaga tcctggccgg ctcgctcacc ctcgaacccg
     4081 tcgacgtctg aaggagaacc ggccatgagc atccaaggca agaagatcac cctgcatgac
     4141 atgacgctgc gcgacggcat gcaccccaag cagcaccaga tgacgctggc gcagatgaag
     4201 tccatcgccc agggcctgga cgccgccggc atgccgctga tcgaagtcac ccacggcgac
     4261 ggcctgggcg gcgcttccgt caactacggc tttccggccc acagcgacga ggaatacctc
     4321 tccgccgtca tcccgctgat gaagcaggcc aaggtgtcgg cgctgctgct gcccggcatc
     4381 ggcaccgtcg accacctgaa gatggcgcgc gagctgggcg tgcacaccat ccgcgtggcc
     4441 acccactgca ccgaggccga tgtcagcgag cagcacatca ccatggcccg caagatggac
     4501 atggacaccg tgggctttct gatgatggcg cacatgaaca gcgccgaggg cctggtcaaa
     4561 caggccaggc tcatggaagg ttacggcgcc aactgcatct acgtgaccga ctcggccggc
     4621 tacctgctgc ccgaccaggt caaggagcgc atcgccgccg tgcgcgccgc actcaagccc
     4681 gaaaccgagc tgggctttca tggccaccac aacctggcca tgggcgtggc caattcgatc
     4741 gccgccatcg aggccggcgc caaccgcatc gacgccgccg ccgccggcct gggcgccggt
     4801 gccggcaaca cgccgatgga ggtgctggtg gccgtgctcg agcgcatggg cgtgcacacc
     4861 ggcgtggacg tgttcaagat ccaggatgtg gccgaggacc tggtggtgcc ggtgatggac
     4921 ttcccgatcc gcatcgaccg cgacgcgctc accctgggct acgccggggt gtacggcagc
     4981 ttcctgctgt tcgccaagcg cgccgaaaag aaatacggcg tgccggcgcg cgagatcctg
     5041 gtcgagatgg gccgccgcgg catggtcg
//
LOCUS       DAOQTZ010000150         5052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_18330_length_5052_cov_12.863718, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000150 DAOQTZ010000000
VERSION     DAOQTZ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18330_length_5052_cov_12.863718"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1747)
                     /locus_tag="PKN51_12675"
     CDS             complement(152..1747)
                     /locus_tag="PKN51_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT85997.1"
                     /translation="MIDMPATDALRRIRRVALGDFIHRSALKFGERTAVVDGDTRLNY
                     RELDARSSRFAHHLIGTIGRGRQVGMLCANSADMVVAYNGIHKSGNVWVPVNIRLDVA
                     GIDYILRHAEVAAVVVDEAFHAAPGVADMLTRLGVPLIVTMAAQAGTAIAGAVTLAQA
                     EAGQSAELPAIDIDSNAPALIMYTSGTTGNPKGAVHSHASVGAALMGNMAGFAVTEQD
                     VFSGVLPLFHCAQHGLAATVHLAGGCIVLLRGFVPDEVRASILRERYTVFTGLPMMYA
                     ALLADPAFRVDSMRLAIYAMAPIPRPLIAQIAGRMTPNVMLATGQTEMYPGTMSFRPL
                     EHPQLDANYWGVSLPHNETAVMDDEGRLLGEGEPGEIVHRGANAMLGYLKDPEATAAA
                     QRFGWHHTGDLGQWGPGGQLMFLDRKKDMIKTGGENVASVKVEAVLLAHPGVAGVGVI
                     GLPHPRWAEAVCAFVVKKPGAEVDEAALQTHCRQHLGGFEVPKLIRFVEQLPATSTGK
                     VQKHLLRREFEQIAAQAWTDEAA"
     gene            complement(1807..2610)
                     /locus_tag="PKN51_12680"
     CDS             complement(1807..2610)
                     /locus_tag="PKN51_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT85998.1"
                     /translation="MNVQFDFSGRHAVVFGGTTGINLGIAQAFARAGARVTVASRKQA
                     NVDAAVATLGDGALGLVADVRDEASVTSALAQAAATHGPIDVLVSGAAGNFLAEVNGM
                     SSNAFKVVVDIDLIGSFHVARQAYAHLRKPGASIIFITAPQAVVAMPHQAHVGAAKAG
                     VDQFARVLALEWGPAGVRVNAISPGPIQGTEGMQRLAPQIQDGDATLRAIVPLGRMGT
                     TQDIAHLAMFLGSDAASYVSGAVIACDGGAQGMLTPMISGAIAAQAPAR"
     gene            complement(2639..>2935)
                     /locus_tag="PKN51_12685"
     CDS             complement(2639..>2935)
                     /locus_tag="PKN51_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT85999.1"
                     /translation="VDMRQRICATAAWLEAVAARADAGDASPDWVAEVCTLKNQATQT
                     MQFCADAGVQILGGMGYMRGTVCERIYREVKVVTIGGGTEEIMKELAARQWGIA"
     assembly_gap    2936..3035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3036..3637)
                     /locus_tag="PKN51_12690"
     CDS             complement(<3036..3637)
                     /locus_tag="PKN51_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT86000.1"
                     /translation="MNDIQAPMSAAELREARSALVETVERFARSEIAPNVSAWDAAGE
                     FPRSLYRRAAELGLLGVGYPEEYGGTPAPYALRLPMWRALCRFGTSGGVQASLFSHNI
                     GLPPVVLLASDAVRQEVVPPVLAGERISALAITEPGGGSDVAQLRTTARREGDEYVVS
                     GEKIFITSGMRADWITLAVRTGESKGASGISLLLVPGDSAG"
     gene            complement(3634..>5052)
                     /locus_tag="PKN51_12695"
     CDS             complement(3634..>5052)
                     /locus_tag="PKN51_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNT86001.1"
                     /translation="ALAGFGNGELLIERAVLQPRHVEVQVFADAHGHAIHLGERDCSV
                     QRRHQKIIEEAPSPAVSPELRARMGACAVALARGAGYVGAGTVEFLLEGQDFYLMEMN
                     TRLQVEHPVTEALTGLDLVEWQIRVARGEALPLAQEQVRHDGHAIEVRLCAEDENYLP
                     HTGRIAHFSAPSATAFAPGPLRFDHAIEGGAEVTPFYDAMLGKLIVHAPTREAAIDAL
                     LGALAQTELLGLPSNRGFLMACLGDARFRRGEALISFLAEHGDALRQSLLKQELLAHD
                     WCALAAIFAREPGTLPCTFATPLRLSHRDEVSALAVRPRAGGLELLGEAPRQIDLQRL
                     PDGAVRCTEAGVARRVRAVAVAGTRPDAPRRWHAQAGGVDWWFDDVSFEPVAGPGAAL
                     AATELRAPFNGRVVRIAAAPGQRLAASEVALVIESMKLEHSLSVRADAQVAELLVAEG
                     QQVSPGQVLLRLAAPATQEAAQ"
BASE COUNT          746 a         1789 c         1689 g          728 t
ORIGIN      
        1 tagcccgcgc tgcgcgccac agcagaaagc cgccccaggg gcggctttct gcttgggggc
       61 ggtgagcgcc agcagggacg cctgccgcct gatgccaaga aaaccgcttt aacgcttgtc
      121 cagcaagcgc caacagctat cgttttgaga gttacgccgc ttcgtcggtc caggcctgcg
      181 cggcgatctg ctcgaactcc cggcgcagca ggtgcttttg caccttgccg gtggaggtgg
      241 cgggcagctg ctcgacgaag cggatcaact tgggcacctc gaagccgccc aggtgctggc
      301 ggcagtgcgt ctgcaacgcg gcttcatcca cctcggcacc gggtttcttg acgacgaagg
      361 cgcacaccgc ctcggcccag cgcgggtgcg gcaggccgat cacgcccacg ccggccacac
      421 ccggatgtgc cagcagcacg gcctcgacct tgacgctggc cacgttttcg ccgccggtct
      481 tgatcatgtc cttcttgcgg tcgaggaaca tcagctggcc gcccggcccc cactggccca
      541 gatcgcccgt gtgatgccag ccaaagcgct gcgcggcggc ggtggcctcg gggtccttca
      601 gatagcccag catggcgttg gcgccgcgat gcacgatctc gcccggctcg ccctcgccca
      661 gcaggcggcc ctcgtcgtcc atcaccgcgg tttcgttgtg cggcagcgac acgccccagt
      721 agttggcgtc cagctgcgga tgctccagcg ggcgaaagct catggtgccg gggtacatct
      781 cggtctggcc ggtggccagc atcacgttgg gcgtcatgcg cccggcgatt tgcgcgatca
      841 gcggcctggg gatgggcgcc atggcgtaga tggccaggcg catcgaatcg acgcggaacg
      901 ccggatcggc cagcagcgcg gcgtacatca tcggcagacc cgtgaacacg gtgtagcgct
      961 cgcgcagtat ggaggcacgc acctcatcgg gcacgaagcc gcgcagcagc acgatgcagc
     1021 cgccggccag atgcaccgtg gccgccaggc cgtgctgggc gcagtgaaac agcggcagca
     1081 cacccgagaa cacgtcctgc tcggtcacgg caaagcccgc catattgccc atcagcgcgg
     1141 cgcccaccga ggcgtgcgag tgcactgcgc ccttggggtt gcccgtggtg ccgctggtgt
     1201 acatgatgag cgcgggggcg ttgctgtcga tatcgatggc gggcagctcg gcgctctgtc
     1261 ccgcttcggc ctgcgccagc gtgacggcgc cggcgatcgc cgtgccggcc tgcgcggcca
     1321 tggtcacgat cagcggcacg cccagcctgg tcagcatgtc ggccaccccc ggcgcggcgt
     1381 ggaaggcctc gtccaccacc accgccgcca cctcggcgtg gcgcaggatg tagtcgattc
     1441 ccgccacgtc gagccggatg ttcaccggca cccagacgtt acccgacttg tgaatgccgt
     1501 tgtaggccac caccatgtcg gccgagttgg cacacagcat gcccacctgc cggccgcggc
     1561 cgatggtgcc gatcaggtga tgggcaaagc gcgagctgcg cgcgtccagc tcgcggtagt
     1621 tgagccgcgt gtcgccatcg accacggcgg tgcgctcgcc gaacttgagt gcgctgcgat
     1681 gaatgaaatc gcccagcgca acgcggcgaa tgcggcgcag ggcatcggtg gcgggcatgt
     1741 cgatcatgaa agtctccgtt ggatgtggct tgaaaacgat gaagaagcaa ctcgccgcac
     1801 cgccactcag cgcgccggcg cctgggccgc gatggcaccg ctgatcatgg gcgtgagcat
     1861 gccctgcgcg ccgccgtcgc aggcaatcac ggcgccggac acgtagctgg cggcgtccga
     1921 accgaggaac atggccagat gggcgatgtc ctgcgtggtg cccatgcggc ccagcggcac
     1981 gatggcgcgc agcgtggcgt cgccgtcctg gatctgcggg gccagccgct gcatgccttc
     2041 ggtgccctgg atcggtccgg gcgagatggc attcacgcgc acgccggcgg ggccccattc
     2101 cagcgccagc acgcgcgcga actggtccac gccggccttg gccgccccca catgggcctg
     2161 atgcggcatg gcgaccaccg cctgcggcgc ggtgatgaag atgatgctgg cgccgggctt
     2221 gcgcaggtgt gcgtacgcct gccgggcaac gtgaaagctg ccgatcaggt cgatgtcgac
     2281 caccaccttg aaggcgttgg acgacatgcc gttgacctcg gccagaaagt tgccggccgc
     2341 gcccgacacc agcacgtcga tcggcccgtg cgtggcggcg gcctgcgcca gcgcgctggt
     2401 cacgctggcc tcgtcgcgca catcggccac cagccccagg gcgccgtcgc ccagggtggc
     2461 caccgccgcg tccacattgg cctgcttgcg actggccacc gtgacccggg cgccggcgcg
     2521 tgcgaacgcc tgcgcaatgc ccaggttgat gccggtggtg ccgccaaaga ccacggcatg
     2581 gcggccgctg aaatcgaatt gaacgttcat gatcgacccc gtggatggcg ggcacgctct
     2641 atgcgatgcc ccactggcgc gccgccagct ccttcatgat ttcttcggtg ccgccaccga
     2701 tggtgacgac cttgacctcg cggtagatgc gctcgcacac cgtgccgcgc atgtagccca
     2761 tgccgcccag gatctgcacg ccggcgtcgg cgcagaactg catcgtctgc gtggcctggt
     2821 tcttcagggt gcagacttcg gccacccagt cgggcgaggc atcgccggcg tcggcgcgcg
     2881 cggccacggc ctccagccag gcggcggtgg cgcagatgcg ctggcgcatg tcgacnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc gctgtcaccc ggcaccagca
     3061 gcaggctgat gccggaagcg cccttggatt cacccgtcct cacggcgaga gtgatccagt
     3121 cggcgcgcat gccgctggtg atgaagatct tctcgccgct caccacgtac tcgtcgccct
     3181 cgcgtcgcgc cgtggtgcgc agctgcgcca cgtccgagcc accgccgggc tcggtgatgg
     3241 ccagtgccga gatgcgctcg cccgccagca ccggcggcac cacctcctgc ctgacggcgt
     3301 cgctggccag cagcaccacc ggcggcaggc cgatgttgtg cgagaacagg ctggcctgca
     3361 ccccgcccga ggtgccaaag cggcacagcg cccgccacat gggcaggcgc agtgcgtagg
     3421 gggcgggcgt gccgccgtac tcctccgggt agcccacacc cagcaaaccc agttcggcgg
     3481 cgcgccggta caggctgcgc ggaaactccc cggccgcgtc ccacgcgctc acgtttggcg
     3541 cgatctcgct gcgtgcaaag cgctcgaccg tctccaccaa cgcgctgcgc gcctcgcgca
     3601 gctcggcagc gctcatgggg gcctggatgt cgttcattgg gctgcctcct gggtcgccgg
     3661 cgcggccaag cgcagcagca cctggcctgg gctgacctgc tggccctcgg cgaccagcag
     3721 ctcggcgacc tgggcgtcgg cgcgcaccga caggctgtgc tcgagcttca tcgactcgat
     3781 caccagcgcc acctcgctgg cggccagccg ctggcccggc gcggcggcga tacgcaccac
     3841 gcggccgttg aagggcgcgc gcagttcggt ggctgccagc gccgcgcccg gcccggccac
     3901 cggttcgaac gacacatcgt cgaaccacca atccacgccg cccgcctgcg cgtgccagcg
     3961 gcgcggcgca tcgggcctgg tgcctgccac ggccacggcg cgcacgcgcc gggccacgcc
     4021 ggcttcggtg cagcgcacgg cgccgtcggg caggcgctgc aggtcgatct gacgcggcgc
     4081 ttcgcccagc agctccaggc cgcccgcgcg cgggcgcacc gccagcgcgc tcacctcatc
     4141 gcggtgcgac aggcgcagcg gcgtcgcgaa ggtgcacggc agggtgccgg gttcacgagc
     4201 aaaaatggcc gccagggcgc accagtcatg cgccagaagc tcctgtttca atagcgactg
     4261 gcgcagcgca tcgccgtgtt cggccaggaa cgaaatcagc gcctcgccgc gccgaaagcg
     4321 cgcgtcaccc aggcaggcca tcagaaagcc gcggttgctg ggcaggccca gcagctcggt
     4381 ctgcgccagc gcgcccagca gggcgtcgat ggccgcctcg cgcgtgggcg cgtgcacgat
     4441 gagcttgccc agcatggcgt cgtagaaggg cgtgacctcg gcaccgcctt cgatggcgtg
     4501 gtcaaagcgc agcggccctg gcgcaaaggc ggtggccgac ggcgctgaaa aatgcgcgat
     4561 ccggcctgtg tgcggcaggt agttctcgtc ctcggcgcac aggcgcacct cgatggcgtg
     4621 gccgtcgtgg cggacctgct cctgcgccag cggcagcgcc tcgccgcgcg ccacgcggat
     4681 ttgccattcg accagatcga ggcccgtcaa cgcctcggtg accgggtgct cgacctgcag
     4741 gcgggtgttc atctccatca aataaaagtc ctgcccctcc agcagaaatt ccaccgtccc
     4801 cgcgcccaca taacccgcgc cgcgcgccag cgccaccgcg catgcgccca tgcgcgcgcg
     4861 cagctcgggc gagacggccg ggctgggcgc ttcctcgatg atcttctgat ggcgcctttg
     4921 caccgagcag tcgcgctcgc ccaggtggat ggcgtggccg tgcgcgtcgg caaacacctg
     4981 cacttcgacg tggcgcggct gcagcacggc gcgctcgatc aacagctcgc cattgccaaa
     5041 gccggccagc gc
//
LOCUS       DAOQTZ010000151         5036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_18402_length_5036_cov_13.615138, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000151 DAOQTZ010000000
VERSION     DAOQTZ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18402_length_5036_cov_13.615138"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(438..1112)
                     /locus_tag="PKN51_12700"
     CDS             complement(438..1112)
                     /locus_tag="PKN51_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT86002.1"
                     /translation="MSARLDHLVVLADTLAQGVAWCEATLGVVPAPGGQHPLFGTHNR
                     LLALGGAAFAQAYLEIIAIDPAAGEPGRTRWFDMDDQRLRRQVRAQGPQLIHWVARVP
                     ELARAAAAWAALGLERGAVLPASRMTPRGLLEWQITVREDGQRLLDGCLPTLIQWGAV
                     HPADALPASGIALESLSLRHPQAALLRAALDAVGLADIVVEPGPVALQARLATPRGAV
                     TLAGFS"
     gene            complement(1109..2011)
                     /locus_tag="PKN51_12705"
     CDS             complement(1109..2011)
                     /locus_tag="PKN51_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNT86003.1"
                     /translation="MARPFAQIDVFTARPGWGNAVAVVLDGQGLDDAAMQRFARWTNL
                     SETTFLLPPTAAGADYALRIFTPGGELPFAGHPTLGSCHAWLAAGGRPGTPGRIVQQC
                     AQGLVELRQHGQRLAFAAPPLRRGDVPPALLTRVVQALGLAPAQVRRAQWLDNGPRWL
                     GLLLDGADTVLALEPDHAALAALGQKVGVAGVHGAPTAIPLIARSSREARAFRPDDAA
                     APRLEVRAFAAPIGVPEDPVTGSLNASLAQWLIDAGELPARYVAAQGAALGRAGRVHV
                     QRDAQGQVWVGGDCVAVIGGEVRL"
     gene            complement(2059..3285)
                     /locus_tag="PKN51_12710"
     CDS             complement(2059..3285)
                     /locus_tag="PKN51_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNT86004.1"
                     /translation="MTQSPWQLAARAETMNPSAIREILKITERPGILNFAGGLPSPET
                     FPVDAMRAACAAVLAEGSAVARPSLQYAASEGLPELRQWVAAELGRQGARVVPEQVLI
                     TNGSQQGLDLVAKVLLDAGSRVLVETPTYLGALQAFTPMQPRFDSVDSDDGGVTASGL
                     REQLQKGERPRFVYLLPNFQNPSGRTQDEARRQQVIAVCREHALPMVEDNPYGELWYD
                     QPPPAPLLARWPEGVIYLGSFSKILAPGLRLGYVVAPPSLYPKLLQAKQAADLHTAGF
                     NQRVVAEVIRDGFLDRHVPTIRARYKTQRDAMLAALARELGSTGATWTRPQGGMFIWV
                     RLPEGLNAQTLLPAAVEAGMAFVPGAPFFAGQSDVRTLRLSFVTSTPAQIERGMAALA
                     SVVRAAMSGTGSGSGA"
     gene            complement(3282..4268)
                     /locus_tag="PKN51_12715"
     CDS             complement(3282..4268)
                     /locus_tag="PKN51_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT86005.1"
                     /translation="MSAQRQARRQRIGFWLGVLGVALFAITLPATRLATGTDAEPQLS
                     PGFVTAARAALAGLLSLAFLLLTRSPWPARAHWRWLLLAVGGNLIGFPLLVALALRSV
                     TAVHGAVVLAVLPLATAAAAALLLGQRERLGFWLCALVGAALVVLYAWLRAQAAGGFG
                     FAAADLLLLGAVLAAAIGYVAGAQLTPQLGAERVICWMCVLGLPLSLPLVAWWWPEAQ
                     TMAQVRAPAWAGLVYVGAVSMWSGFFAWFRGLDWGGALRVSQVLLLQPFLTMLFAVPL
                     LGERLDALSLAFAAAVVLTVFVGRRLSRPTQAGAPLNGREPQTLSPPKGQQP"
     gene            complement(4265..4897)
                     /locus_tag="PKN51_12720"
     CDS             complement(4265..4897)
                     /locus_tag="PKN51_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNT86006.1"
                     /translation="MTTAEFSALLLLATASTFTPGPNTTLSTAIAANRGLRGALRFVC
                     AVPAGWALLLGLCGVGLGALVMALPLLRWGIVVTGVGYMLWLAARLARSATLAEADAR
                     RLDVSFVEGVGLQFLNIKAWMLALTVVAGWVAGRPDWPARLLLVLPVMLAFGFSSNFT
                     YALIGSLLRGWLARGRRLLWFNRLMALALVLTAGWMAWSAMALMSGAPLP"
BASE COUNT          794 a         1878 c         1790 g          574 t
ORIGIN      
        1 gcgcgccggc gtcaaaccgg cgacagcgcc ggtcatccta gaaacggcag ttggacgccc
       61 ccgcccgcgc cgtgatcggt gtgccaggca agacgtgacg acgcagcgca ctcccgtgcg
      121 caaggaggag caacgccgca tggcgcggcg agcgcggtgc gggtgggtgc gtagcgtgct
      181 cacccaggag gtgaaggcca tcgcagccgc cagaccccgc atcgatgcgg tctgcccgaa
      241 gttgacaagc ggtcaactgc cgtttctagg acaacagggc ccagcggtac tgcggcgtgg
      301 gcggcatggc gcggtacacc acctgggccg cggcttcgat ctcggcaagg ccggggagcg
      361 tcggttcgga ggacatgaat tactctctta ttggtagctg ctcgcgcttg actggagcgc
      421 gccagagcca cttttgacta tgaaaaacca gccagggtga cggcgccgcg cggcgtggcc
      481 aggcgcgcct gcagggcgac gggcccgggc tccacgacga tatccgccag gcccacggca
      541 tccagcgcgg cgcgcagcag cgcggcctgc gggtgacgca ggctgagcga ttccagcgcg
      601 atgccgctgg ccggcagagc gtcggcggga tgcaccgcgc cccactggat cagggtcggc
      661 agacagccgt ccagcaggcg ctggccatcc tcccgcacgg tgatctgcca ctccagcagg
      721 ccgcgcggcg tcatgcggct ggctggcagc accgcgccac gctcgagccc cagcgcggcc
      781 caggctgcgg cggcgcgcgc cagctcgggt acgcgcgcca cccagtggat cagctgcggg
      841 ccctgcgcgc gcacctgccg gcgcaggcgt tggtcgtcca tgtcgaacca gcgcgtgcgg
      901 cccggctcgc ccgctgccgg atcgatcgcg atgatctcaa ggtaagcctg cgcaaaggcc
      961 gcgccgccca gggccagcag ccggttgtgg gtgccaaaca gcgggtgctg gccgcccggg
     1021 gcgggcacca cgcccagcgt ggcctcgcac caggcgacgc cctgcgccag cgtgtcggcc
     1081 agcaccacca gatggtccag ccgcgcactc acagccgcac ctcgccgccg atcaccgcca
     1141 cgcagtcgcc acccacccac acctggccct gggcgtcgcg ctgcacgtgc acgcgcccgg
     1201 cgcgcccgag cgccgcgccc tgcgccgcca cgtagcgcgc gggcagctcg ccggcgtcga
     1261 tcagccactg cgccaggctg gcgttgaggc tgccggtgac cgggtcttcc ggcacgccga
     1321 tgggcgcggc aaaggcgcgc acctccagcc gcggggcggc ggcgtcgtcg ggcctgaagg
     1381 cgcgcgcttc acggctcgat cgcgctatca atggtatagc tgtcggcgcg ccatggacgc
     1441 cggcgacgcc gactttctgc cccaaggctg cgagggcggc gtggtccggc tcgagcgcca
     1501 gcacggtgtc ggcgccgtcc agcagcagac ccagccagcg cggcccgttg tccagccact
     1561 gggcgcgccg cacctgggcc ggtgccagac ccagcgcctg caccacgcgc gtcagcagcg
     1621 ccggcgggac gtcgccgcgc cgcagcggcg gcgcggcaaa ggccaggcgc tggccgtgct
     1681 ggcgcagctc gaccaggccc tgggcgcact gctgcacgat gcgcccgggc gtgcccggcc
     1741 tgccgcccgc cgccagccag gcatggcagc tgcccagcgt ggggtggccg gcaaagggca
     1801 gctcgccgcc gggggtgaag atgcgcagcg cgtagtcggc gccggccgcc gtcggcggca
     1861 gcaggaaggt ggtttcggac aggttggtcc agcgcgcaaa gcgctgcatg gcggcgtcgt
     1921 ccagcccctg gccgtcgagc accaccgcca ccgcattgcc ccagccggga cgggcggtga
     1981 aaacgtcgat ctgcgcgaag ggacgtgcca tggagaacct cggttgctac gctatcgata
     2041 gctgcttacg cttgtccatc atgcgccaga gcccgatccg gtgcctgaca tggcggcgcg
     2101 cacgacgctg gccagcgccg ccatgccgcg ctcgatctgc gccggcgtcg aggtgacgaa
     2161 cgacaggcgc agcgtgcgca cgtcggactg gccggcaaag aacggcgcgc ccggcacgaa
     2221 ggccatgccg gcctcgaccg cggccggcag caacgtctgg gcgttcaggc cttcgggcag
     2281 acgtacccag atgaacatgc cgccctgcgg gcgcgtccag gtggcgccgg tggagcccag
     2341 ctcgcgcgcc agcgccgcca gcatggcgtc gcgctgcgtc ttgtagcggg cccggatggt
     2401 gggcacatgc cgatccagaa aaccgtcccg gatcacctcg gccaccacgc gctggttgaa
     2461 gcccgccgtg tgcaggtcgg ccgcctgctt ggcctgcagc aacttggggt agagcgaagg
     2521 cggcgccacc acgtagccca gacgcaggcc gggcgcgagg atcttggaaa acgagcccag
     2581 gtagatcacc ccttcgggcc agcgcgccag cagcggcgcc ggcggcggct ggtcgtacca
     2641 cagctcgccg taggggttgt cttcgaccat gggcagcgcg tgctcgcggc agaccgcgat
     2701 cacctgctgg cgccgcgcct cgtcctgcgt gcgcccgctc gggttctgga agtttggcaa
     2761 caggtagacg aaccgcggtc gctccccttt ttgtagctgc tcgcgcagac ccgatgcggt
     2821 gacaccgcca tcgtcgctgt ccacgctgtc gaaccgcggc tgcatcgggg tgaacgcctg
     2881 caacgccccc aggtaggtcg gtgtctccac cagcacacgg ctgccggcgt ccagcagcac
     2941 cttggccacc aggtccagac cctgctggct gccgttggtg atcagcacct gctcgggcac
     3001 cacgcgcgcg ccctggcggc ccagctcggc cgccacccac tggcgcagct cgggcaggcc
     3061 ctcgctggcg gcgtattgca gcgacgggcg cgccacggcg ctgccttcgg ccagcaccgc
     3121 ggcgcaggcg gcgcgcatgg catccaccgg gaaggtctcc ggcgacggca ggccgccggc
     3181 gaagttgagg atgccggggc gctcggtgat cttcagaatt tcgcggatcg ccgacgggtt
     3241 catggtttcc gcgcgcgccg ccagttgcca tggtgactgg gtcatggttg ttgccccttg
     3301 ggtggtgaaa gcgtctgcgg ctcgcgcccg ttcaggggcg cgcccgcctg ggtgggccgc
     3361 gacaggcgcc ggccgacgaa cacggtgagc accaccgccg ccgcgaaggc aagcgaaagc
     3421 gcatccagcc gctcgcccag caacggcacg gcgaacagca tggtcagaaa gggttgcagc
     3481 agcagcacct ggctgacgcg cagcgcgccg ccccagtcga ggccgcgaaa ccaggcgaag
     3541 aagcccgacc acatggacac cgcgcccacg tagaccaggc cggcccaggc cggcgcgcgc
     3601 acctgcgcca tggtctgtgc ctcgggccac caccacgcca ccagcggcag cgacagcggc
     3661 aggcccagca cacacatcca gcagatcacc cgctcggcgc cgagctgcgg cgtcagctgg
     3721 gcaccggcca cgtagcctat ggcggcggcg agcacggcgc ccagcagcag cagatcggcc
     3781 gccgcaaagc caaagccgcc ggccgcctgc gcgcgcagcc aggcgtacag caccaccagc
     3841 gcagcaccca ccagggcgca cagccagaag cccagccgct cgcgctgccc cagcagcagc
     3901 gccgcagcgg ccgccgtcgc cagcggcagc accgccagaa ccaccgcgcc gtgcaccgcc
     3961 gtcacgctgc gcagcgccag cgccaccagc agcggaaagc cgatcaggtt gccgccgacg
     4021 gccagcagca gccaccgcca gtgcgcgcgc gccggccagg gcgagcgcgt gagcagcagg
     4081 aaggccagcg acagcaggcc ggccagcgcc gcccgcgccg ccgtcacgaa gccgggcgac
     4141 agctgcggct cggcatccgt gccggtggcc aggcgcgtgg ccggcagggt gatggcaaac
     4201 agcgccaccc ccagcacgcc cagccagaag ccgatgcgct ggcgccgtgc ctgccgctgc
     4261 gcgctcatgg gagcggtgcg ccggacatca gcgccatggc cgaccacgcc atccagccgg
     4321 cggtcagcac cagggccagc gccatgaggc ggttgaacca cagcaggcgg cgcccccgcg
     4381 ccagccagcc gcgcagcaac gagccgatca gcgcgtaggt gaagttgctg gaaaagccga
     4441 acgccagcat caccggcagc accagcagca gccgcgctgg ccagtccggc cgcccggcca
     4501 cccagccggc caccacggtg agcgccagca tccaggcctt gatgttgaga aattgcaggc
     4561 ccacgccctc gacaaagctg acgtcgagcc ggcgcgcgtc ggcttcggcc agcgtggcgc
     4621 tgcgcgccag ccgcgccgcc agccacagca tgtagcccac gcccgtcacc acgatgcccc
     4681 agcgcagcag cggcagcgcc atcaccagcg cccccaggcc gacgccacac aggcccagca
     4741 gcagcgccca gccggccggc accgcgcaga cgaagcgcag cgccccgcgc aggccgcggt
     4801 tggcggcgat ggcggtggac agggtggtgt tgggcccggg ggtgaaggtg ctggcggtgg
     4861 ccagcagcag cagggcactg aattcggcgg tcgtcacggc aggcggcgca caggcgaacc
     4921 cgacatgggc gcggcggcgc ggcccatggc gctcagaacg ccgccatgcc ggtgatggcg
     4981 cggcccagga tcagcgcgtg gatgtcgtgc gtgccctcgt aggtgttgac cacctc
//
LOCUS       DAOQTZ010000152         5017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_18504_length_5017_cov_11.950625, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000152 DAOQTZ010000000
VERSION     DAOQTZ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18504_length_5017_cov_11.950625"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKN51_12725"
     CDS             complement(<1..272)
                     /locus_tag="PKN51_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT86007.1"
                     /translation="MTTLSPPTASPRAIRFYHRGTLVSVDGVPVTRTVLDWLREQRRL
                     TGTKEGCNEGDCGACTVVLGELDASGQLRLSSVNACIQFLPTLDGKA"
     gene            complement(460..1806)
                     /gene="argA"
                     /locus_tag="PKN51_12730"
     CDS             complement(460..1806)
                     /gene="argA"
                     /locus_tag="PKN51_12730"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNT86008.1"
                     /translation="MSTVFNFTFVPWFRSVAPYIHMHRGKTFVVGVVGEAIAAGKLAA
                     IATDLALIQSMGVKIVLVHGFRPQVNEQLQAKGHEPRYAQGMRITDEVALDCAQEAAG
                     QLRYEIEAAFSQGLPNTPMAGSRVRVISGNFLTARPMGIIDGVDFQSTGVVRKVDAAG
                     IRHSLDSGALVLISPFGFSPTGEAFNLRMEEVATSVAIALQADKLLFLTEVPGIRVRP
                     ESPAAEDNPIDTEIPLADAQALLARLPAPEQPTDTAFYLRHCVSACLHGVERSHILPY
                     ATDGALLLEIYVHDGIGSMVVDEKLESLREAGADDVTGILHLIEPFEKDGTLVKRSRT
                     DIERDIANYTILEHDGVIFACAALYPYPEAATGEMAALTVSPQSQGQGDGEKVLRRIE
                     QRAREMGLKSIFVLTTRTMHWFIKRGFQQVDPDWLPEARKRKYNWDRRSQVLVKHL"
     gene            complement(2003..2515)
                     /locus_tag="PKN51_12735"
     CDS             complement(2003..2515)
                     /locus_tag="PKN51_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNT86009.1"
                     /translation="MKSMEHFLERLMFASRWLLAPFYIGLVGAIAILLVKFVKEFVAL
                     LPQVLTLEGGQVMVGVLTLVDVALVANLLLIIVFAGYENFVSKINTGDSEDRPEWMGH
                     VTFSDLKIKLIGSIVAISGIELLKAFVNIDSHSNQQIAWKIALHMTFVLSGVLFAVMD
                     RLTAGKSGGH"
     gene            complement(2815..3942)
                     /locus_tag="PKN51_12740"
     CDS             complement(2815..3942)
                     /locus_tag="PKN51_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86010.1"
                     /translation="MTLSPAPTRACRLRTLQRGIAILSLGALIAGCGGGSGDGATPPA
                     PAPTPAPAPAPAPSPAPAPAPSPGNATYGDLRAAALGVGASLNGALPFPADNAWNTDI
                     SARPVDPNSDALIASIGLDRGLHPDFGAGLYQGQPIGIPYVVVPGTQPRVAVQFTDYG
                     DESDPGPYPIPPDAPIEGGPASSGDRHVIVIDRDAGRLYEMGNSYPQADGSWRASGGA
                     VFHLDSNAVRPTAQPGWTSADAAGLPIFPGLVRYDEAALGPGGIRHALRFTVSRTRRA
                     YVPPATHWASSNTSADLPPMGMRVRLKASYVIPAGFSAETRAILTALKTYGMLVADNG
                     SNWYLSGAPDARWNNDRLVSELGQVAGRHFEVVRMEGVVTP"
     gene            4222..4557
                     /locus_tag="PKN51_12745"
     CDS             4222..4557
                     /locus_tag="PKN51_12745"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNT86011.1"
                     /translation="MTYQNDRNQCLATPSIRQLEKRIQSGAADSHHVAFTKHALARMR
                     QRGITRIMVLDALRLGCIRQQPEPDIRHPGVKCRMERLVSGVLVGAVVYLEYPAPDLT
                     VVTVIDLGV"
     gene            4560..>5017
                     /locus_tag="PKN51_12750"
     CDS             4560..>5017
                     /locus_tag="PKN51_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86012.1"
                     /translation="MYHYTDGGLRNVWLGNGYTIRDTPYGQAVAIQDLEGLTAAICDA
                     FVRQPKPLTQTEFRYLRTSGLQLSQPALGAALGVDAQTIARWEKGAYIPKMADKLVRL
                     FYLNHANGNVRLSSAFESLRAVERAAHGPQPMRVIAEAKGRHWGARLESDG"
BASE COUNT          810 a         1638 c         1758 g          811 t
ORIGIN      
        1 gccttgccgt ccagcgtggg caggaactgg atgcaggcgt tcacgctgct caggcgcagc
       61 tggccgctgg cgtcgagttc gcccaacacc acggtgcagg cgccgcagtc gccttcattg
      121 cagccttcct tggtgccggt caggcgccgc tgctcgcgca gccagtcgag cacggttcgg
      181 gtgaccggca cgccgtccac cgacaccagc gtgccgcggt gatagaagcg gatggcccgg
      241 gggcttgcgg tcgggggact gagggtcgtc atctgcggct ccggcggtga aggtctgccc
      301 cggggatggg tcagccaacc gttgcgcccg cgatggtgcg cgggctggcc gccccgtggc
      361 atacaggggg cacgatagcg gatgtatggg tcgcgttatg actcgggggt taaccccgag
      421 aggggcgcag ggtcggcggg cgctgggtgc tgccggcgtt cacaggtgct tgaccagcac
      481 ctggctgcgg cggtcccagt tgtacttgcg cttgcgcgcc tcgggcagcc agtcggggtc
      541 gacctgctgg aagccgcgct tgatgaacca gtgcatggtg cgcgtggtca gcacgaagat
      601 gctcttgagc cccatttcgc gtgcgcgctg ctcgatgcgc ctgagcacct tttcgccgtc
      661 gccctggccc tggctttgtg gcgacaccgt gagcgcggcc atttcgccgg tggcggcttc
      721 ggggtagggg tagagcgcgg cgcaggcgaa gatcacgccg tcgtgctcca ggatggtgta
      781 gttggcgatg tcgcgctcga tgtcggtgcg gctgcgcttg accagggtgc cgtccttctc
      841 gaacggctcg atcaggtgca ggatgccggt gacgtcgtcg gcgccggctt cgcgcaggct
      901 ttccagcttc tcgtcgacca ccatgctgcc gatgccgtcg tgcacataga tttccagcag
      961 cagcgcgccg tcggtcgcgt agggcaggat gtggctgcgc tccaccccgt gcagacaggc
     1021 gctgacgcag tggcgcaggt agaacgcggt gtcggtgggc tgctcggggg cgggcagccg
     1081 cgccagcagc gcctgcgcgt cggccagcgg gatttcggtg tcgatcgggt tgtcttcggc
     1141 ggccgggctt tcggggcgca cgcggatgcc gggcacctcg gtcaggaaca gcagcttgtc
     1201 ggcctgcagg gcgatggcca ccgacgtcgc cacttcttcc atgcgcaggt tgaaggcctc
     1261 gcccgtgggc gaaaagccga acggtgaaat cagcaccagc gcgcccgaat ccagcgagtg
     1321 gcgaatgccg gcggcgtcca ccttgcgcac caccccggtg ctctgaaagt cgacgccgtc
     1381 gatgatgccc atgggccgcg cggtgaggaa gttgcccgag atcacgcgca cccgcgagcc
     1441 ggccatcggc gtgttgggca ggccctggct gaaggcggcc tcgatctcgt agcgcagctg
     1501 cccggcggcc tcctgggcgc agtccagcgc cacctcgtcg gtgatgcgca tgccctgcgc
     1561 atagcgcggt tcgtgcccct tggcctgcag ctgctcgttg acctgggggc gaaagccgtg
     1621 caccagcacg atcttcacgc ccatgctctg gatcagcgcc agatcggtgg cgatggccgc
     1681 cagcttgccg gcggcgatgg cttcgcccac cacccccacc acgaaggttt tgccgcggtg
     1741 catgtggatg tacggcgcca ccgagcggaa ccagggcacg aaagtgaagt tgaagacggt
     1801 ggacatgcgc ggcctgaagg ggtgcgtgaa gcccggccca ggggccggct gccgcacagt
     1861 gtaacgatcc gcgcgcaccg gcggcggccc tcgcggtgct gcacaactat gaaaacagta
     1921 gctggtggcg cttgccagac aagcgccaga ggccaatctg acggtgcaac gcgccagccc
     1981 ggccggtgcg ccccggtggc cgtcagtgac cgcccgactt gcccgccgtc aggcggtcca
     2041 tcacggcaaa cagcacgccc gagagcacaa aggtcatgtg cagcgcgatc ttccaggcga
     2101 tctgctggtt gctgtggctg tcgatgttga cgaaggcctt cagcagttca atgcccgaaa
     2161 tcgccacgat cgagccgatg agcttgatct tcaggtcgga aaaagtgacg tgccccatcc
     2221 attccggccg atcctcgctg tcgccggtgt tgatcttgga gacaaagttt tcatagccgg
     2281 caaacacgat gatgagcagc aggttggcca ccagggccac gtcgaccagc gtcagcaccc
     2341 cgaccatgac ctgcccgccc tccagcgtca gcacctgcgg cagcagggcg acgaactcct
     2401 tcacgaactt gaccagcagg atggcgatgg cgcccaccag accgatatag aaaggcgcca
     2461 gcagccagcg gctggcgaac atgaggcgtt ccagaaaatg ttccattgat ttcatgatgc
     2521 agtgcatgcc ataggttcaa gccccggcgc cggggaaggg ccacggccca cctggcgcag
     2581 tcgccacggc aagccatgca gtgccgccag ggcggctcga cacttctgaa atgatagctt
     2641 ctggcgcttg ccatgcccgg gctggcgccc agaacgaccc gtaattcgca gaccaacccg
     2701 ggcggtccgg gcccgcgtgc ccgcccgcag ggcccggggg cgccccgatg gccgatggct
     2761 gcccacggca ggcccaggtc ggcgccacct tgcgaaggcg ggcgcaggcg gcccctacgg
     2821 cgtcaccacc ccctccatcc gcaccacctc gaagtggcgg cccgccacct ggcccagctc
     2881 gctcaccaga cggtcgttgt tccagcgcgc gtcgggcgcg ccgctcaggt accagttgga
     2941 gccgttgtcg gccaccagca tcccgtaggt cttcagcgcc gtgaggatgg cgcgcgtttc
     3001 ggccgagaag ccggcgggga tcacgtagct ggccttcagc cgcacgcgca tgcccatggg
     3061 cggcaggtcg gcgctggtgt tgctggatgc ccagtgcgtg gccggcggca cgtaggcgcg
     3121 gcgcgtgcgg ctcaccgtaa agcgcagggc gtgccggatg ccgcccgggc ccagcgcggc
     3181 ttcgtcgtag cgcaccaggc cgggaaagat gggcaggccg gccgcgtcgg cgctggtcca
     3241 gcccggctgg gcggtggggc gtacggcgtt gctgtccagg tggaacaccg cgccgcccga
     3301 ggcgcgccag ctcccatctg cctgcgggta gctgttgccc atctcgtaca ggcggcccgc
     3361 gtcgcggtcg atcacgatca cgtgccggtc gccgctgctg gccggcccgc cctcgatcgg
     3421 cgcatcgggc ggaatggggt aggggccggg gtcgctctcg tcgccatagt cggtgaactg
     3481 caccgccacg cgcggctgcg tgcccggcac caccacgtag gggatgccga tcggctggcc
     3541 ctggtacagc ccggcgccaa agtccgggtg caggccgcgg tccagcccga tgctggcgat
     3601 cagcgcgtcg gagttcggat cgaccgggcg cgcgctgatg tcggtgttcc aggcgttgtc
     3661 ggccgggaag ggcagcgccc cgttcagcga cgcgcccacg cccagcgcgg cggcgcgcag
     3721 gtcgccgtag gtggcgttgc cgggggaggg tgcgggtgcc ggggccgggg acggggcggg
     3781 agcgggagcg ggagctggcg tgggtgctgg agcgggcggc gtggcgccat ccccgctgcc
     3841 accaccgcaa cctgcgatca gcgcgcccag gctgaggatg gcgatgccac gttgcagggt
     3901 gcgcaagcgg catgcgcggg tgggtgcagg agagagagtc atgggcgtca tcttgaagga
     3961 tggagcagtg tgcccgagcc ggcgccttcg ccaccgtgtg gatgcgtttc gtgcgcggtg
     4021 gaatggtgag aaagcgtgta gagtccgtag attcttcaac ccaaatcggc ccccagcgct
     4081 ttaccatcaa gcgcaagcag ctatcagttc aatagtaatt ggctttgccg cgatggtccc
     4141 tcccattccc cgttccgccg tgggagtggc ggcacacagc aggggtaatc ccaacccaat
     4201 cagccagcca gatgcggtaa catgacctat caaaatgata ggaaccaatg tttggccacc
     4261 ccatctatca ggcagctcga aaagcggatt caaagcggcg ccgcagacag ccaccatgtt
     4321 gcctttacca agcacgcgct ggcgcgcatg aggcagcgcg gcatcacgcg aatcatggtg
     4381 ctggatgcgc tgcggctggg gtgcatacgc cagcagccgg agccagacat caggcacccc
     4441 ggcgtcaagt gccgcatgga acggctggtt tccggcgtgc tggtgggagc cgtggtgtat
     4501 ctggagtatc ccgcccctga tttgacagta gtcacggtga ttgatttagg agtctgagca
     4561 tgtaccacta caccgacggc ggattgcgca acgtgtggct tggcaacggc tacaccatcc
     4621 gcgacacacc ctatgggcaa gccgttgcca tccaggacct ggagggcctg actgcggcca
     4681 tctgcgatgc cttcgtgcgg caacccaagc ccctcacgca aaccgagttt cgctacctgc
     4741 gcacctccgg actgcaactg tcacagccag cgctgggcgc agcgctgggg gttgacgcgc
     4801 agaccattgc tcgctgggag aaaggggcat atatccccaa aatggccgac aagctcgtgc
     4861 ggctgttcta cctcaatcac gccaacggca acgtccgcct gagcagcgca ttcgagtccc
     4921 tgcgcgccgt cgagcgcgcc gcgcacgggc cgcagcccat gcgggtcatt gccgaggcca
     4981 agggtcggca ctggggagcc cgcctggaaa gcgacgg
//
LOCUS       DAOQTZ010000153         4996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_18618_length_4996_cov_12.941510, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000153 DAOQTZ010000000
VERSION     DAOQTZ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18618_length_4996_cov_12.941510"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKN51_12755"
     CDS             <1..987
                     /locus_tag="PKN51_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT86013.1"
                     /translation="SPRTVGIAAAVVTVAIWTGFIVIGRASAAHTLLPFDIALLRILG
                     AGAVLLPWGWLLARRAAPGQRSLLGFSPLPLRPTLLTGLFGGLVYAILCYAGFFYAPA
                     AHASVLMPGSLPLWTALLALPLLGERISRPRALGLACIVAGDLAVGGSGLLSAFDGGQ
                     VWKGDLIFMGAAFCWSVYSVLARRFGLDPVRATIAITALAFFVYVPAFALLAAAGLLP
                     TRLAQAPWGELLFQALFQGVGSVVISGITFVQMVRVFGPVRSTMITALVPGLSALGAV
                     LLLGEPLGLNLLAGLALVTAGILFGVRAARPVTPDSIAVGARQTSAGAVKAS"
     gene            984..1712
                     /gene="tsaB"
                     /locus_tag="PKN51_12760"
     CDS             984..1712
                     /gene="tsaB"
                     /locus_tag="PKN51_12760"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNT86014.1"
                     /translation="MNQTAFNLLAFDTSTEQLSVAVQRGADGRRWAHQGAGGAQASVT
                     LIPTVQRLLAEAGLALGDLHAIVFGRGPGSFTGLRTACAVAQGLAWGAGLPVLPLDTL
                     LAVAEAARQSAGATRVLALLDARMGEVYHAAYAWSADHGWQTLAEPAVCAPERLPAPP
                     PGAVLAGNAMAVHGERLPPALRALPQVHALPGAQALLTLAPAGLTAGRAVPADQALPL
                     YIRDKVAQTTAERAAARSAPTQTP"
     gene            1709..2209
                     /gene="rimI"
                     /locus_tag="PKN51_12765"
     CDS             1709..2209
                     /gene="rimI"
                     /locus_tag="PKN51_12765"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNT86015.1"
                     /translation="MSTLLSPPSPAPAAALEAHFAPMTPERLDAVLAVEQSAYSHPWS
                     RGNFRDSLAAGHQAQCLLAGDELLGYFVAMLGHREVHLLNITVAPVHQRQGWALVMLD
                     ALALWSRARQAEWLWLEVRASNTRAQQVYLRHGFARVGLRKGYYPDGHGLREDAVVMN
                     LRLEPA"
     gene            2235..3149
                     /locus_tag="PKN51_12770"
     CDS             2235..3149
                     /locus_tag="PKN51_12770"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNT86016.1"
                     /translation="MTLALDARQRAMLAEMGIRLWQRAPQPVTLEAPETIAAAADEAR
                     PSKFFDAKSAGGAAPPPMTPRRMAEAAAPAPAPAAAPASALGPRPEGVEAMDWPQLQA
                     AVATCRACGLCAGRTQTVFGAGSRRADWMVVGEAPGETEDRQGQPFVGPAGQLLDAML
                     AAIGLSRLADDAMNSVAFGAEPADAAGPNGLKRGVYIANVLKCRPPGNRNPQPDEIAQ
                     CEPYLRRQVALVQPKVILAMGRFAVQSLLQTGEPIGRLRGRVHHYAGVPVIVTYHPAY
                     LLRALPDKAKAWQDLCLALEVMRGSAPG"
     gene            complement(3324..>4996)
                     /locus_tag="PKN51_12775"
     CDS             complement(3324..>4996)
                     /locus_tag="PKN51_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNT86017.1"
                     /translation="VLCFTNNIPQRDGGTHLTGLRAAMTRVITKYIADNELAKKAKVD
                     VSGDDLREGLVCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEDIVGKALAEFLEERPND
                     AKIVCGKIVEAARAREAARKAREMTRRKGVLDGFGLPGKLADCQEKDPAQCEIYIVEG
                     DSAGGSAKQGRDRKFQAILPLRGKILNVERARYEKLLTSNEIVTLITALGTGIGRSAE
                     DGNGNGSTKSGADDFNIDKLRYHRVIIMTDADVDGAHIRTLLLTFFYRQMPELVERGH
                     IYIAQPPLYKVKNGKEELYLKDGAALDAFLLRVALKDAVVHTGGAAPRQLSGDTLDEL
                     ARRHQLAEAVIERLSAFMDTEALRAIAAGVTLNLDTVEAAQASAAVLQQHLHAEQANG
                     SAPEVSGEFDPRSDKPVLRISRRHHGNVRHSVITQDFVHGADYAALAEAAQTFRDLLG
                     EGARVRRGEGERQREQAVASFREAMAWLIGEAERTTTRQRYKGLGEMNPEQLWETTMD
                     PAVRRLLRVQIEDAIEADRVFTTLMGDEVEPRRAFIETNALRALNIDA"
BASE COUNT          572 a         1781 c         1818 g          825 t
ORIGIN      
        1 tctccccgca cggtcggcat cgcggcggcc gtggtcacgg tggcgatctg gaccggcttc
       61 atcgtcatcg gccgcgcctc ggcggcgcac accctgctgc cgttcgacat cgccctgctg
      121 cgcatcctgg gtgcgggtgc ggtgttgctg ccctggggct ggctgctggc gcggcgcgcc
      181 gcgccggggc agcgttcgct gctgggcttt tcgccgctgc cgctgcgccc cacgctgctg
      241 accgggctgt tcggcgggct ggtgtacgcc atcctgtgct atgcgggctt cttctacgcg
      301 ccggcggcgc acgcctcggt gctgatgccg ggcagcctgc cgctgtggac ggcgctgctg
      361 gcgctgccgc tgctgggcga gcgcatctcg cgcccacgcg cgctcggtct ggcctgcatc
      421 gtggcgggag atctggcggt gggcggcagc ggcctgctgt cggcgttcga cggcggtcag
      481 gtgtggaagg gcgacctgat cttcatgggc gcggccttct gctggtcggt ctacagcgtg
      541 ctggcgcgcc gcttcgggct cgatccggtg cgcgccacca tcgccatcac ggcgctggcg
      601 ttcttcgtct acgtgccggc gttcgccctg ctggccgctg ccgggctgct gcccacgcgc
      661 ctggcgcagg cgccgtgggg cgagctgctg tttcaggcgc tgttccaggg cgtcggctcg
      721 gtggtgattt cgggcatcac cttcgtgcag atggtgcgcg tgttcgggcc ggtgcgctcg
      781 accatgatca ccgccctggt gcccgggctg tcggcgctgg gggcggtgct gctgctgggc
      841 gagccgctgg gcctgaacct gctggccggg ctggcgctgg tgaccgccgg catcctgttc
      901 ggggtgcggg cggcgcgccc ggtcactcct gattcgatag ctgttggcgc acgccagaca
      961 agcgctggag ccgtaaaagc ctcatgaacc agacggcctt caacctgctg gccttcgaca
     1021 ccagcaccga gcagctgtcg gtggcggtgc agcgcggcgc cgacggccgc cgctgggcgc
     1081 accagggcgc cggcggggcg caggcctcgg tcacgctgat ccccacggtg cagcggctgc
     1141 tggccgaagc cgggctggcg ctgggcgatc tgcacgccat cgtgttcggg cgcgggccgg
     1201 gctcgttcac cgggctgcgc accgcctgcg cggtggcgca ggggctggcc tggggtgccg
     1261 gcctgccggt gctgccgctg gacaccctgc tggcggtggc cgaagcggcg cgccagtcgg
     1321 ccggcgccac ccgggtgctg gcgctgctgg acgcgcgcat gggcgaggtc taccacgccg
     1381 cctacgcctg gtccgccgac cacggctggc agaccctggc cgagccggcg gtctgcgcgc
     1441 ccgagcggct gccggcgccg ccgcccgggg cggtgctggc cggcaacgcc atggccgttc
     1501 atggcgagcg cctgccgccc gcgctgcggg cgctgccgca ggtgcacgcc ctgcccggcg
     1561 cccaggcgct gctgacgctg gcgccggccg gcctgaccgc cggtcgggcg gtgccggccg
     1621 atcaggcgct gccgctctac atccgcgaca aagtggcgca gactacggcc gagcgcgctg
     1681 ccgcccgcag cgccccgacc cagaccccat gagcaccctg ctgtcgcccc cttcccccgc
     1741 ccccgcggcc gcactggagg cccatttcgc ccccatgacg cccgagcggc tggacgccgt
     1801 gctggcggtc gagcaaagcg cctacagcca cccctggtcg cgcggcaact tccgcgacag
     1861 cctggccgcc ggccaccagg cgcagtgcct gctggcgggc gacgagctgc tgggctactt
     1921 cgtcgccatg ctcggtcatc gcgaggtgca cctgctcaac atcacggtgg cgccggtcca
     1981 ccagcgccag ggttgggcgc tggtgatgct ggacgcgctg gcgctgtggt cgcgcgcgcg
     2041 ccaggccgaa tggctgtggc tggaagtgcg cgccagcaac acccgcgcgc agcaggtgta
     2101 cctgcgccac ggctttgccc gcgtcggcct gcgcaagggc tactaccccg acggccacgg
     2161 cctgcgcgaa gacgccgtgg tcatgaacct gcgcctggag cccgcctgat ggcgccgtcg
     2221 ccgcggatcg ttccatgacc ctggcgctgg acgcacgcca gcgcgccatg ctggccgaaa
     2281 tgggcatccg gctgtggcag cgcgcgcccc agcccgtcac cctggaagct cctgaaacaa
     2341 tagctgctgc cgcagatgag gcgcggccta gcaagttttt tgacgcaaaa tccgcggggg
     2401 gcgcggcgcc ccccccgatg accccacggc gcatggccga agcggccgcc ccggcgccag
     2461 cgccggccgc cgcgcccgcc agcgccctgg gcccgcgccc ggaaggcgtg gaggccatgg
     2521 actggccgca actgcaggcc gccgtcgcca cctgccgcgc ctgcggcctg tgcgccgggc
     2581 gcacccaaac cgtgttcggc gccggcagcc ggcgcgccga ctggatggtg gtgggcgaag
     2641 ccccgggcga aaccgaggac cgccagggcc agcccttcgt cggccccgcc ggccagttgc
     2701 tggacgccat gctggccgcc atcgggctct cgcggctggc cgacgacgct atgaattcag
     2761 tagctttcgg cgctgaacca gcggacgctg caggccccaa cggcttgaag cgcggggtct
     2821 acatcgcgaa cgtcctgaag tgccgcccgc ccggcaaccg caacccgcag ccggacgaaa
     2881 tcgcccagtg cgaaccctat ctgcgccgcc aggtcgcgct ggtgcagccc aaggtcattc
     2941 tggccatggg ccgctttgcc gtgcagtcgc tgctgcagac cggcgagccc atcggccgcc
     3001 tgcgcggccg cgtgcaccac tacgccggcg tgcccgtgat cgtcacctac cacccggcct
     3061 acctgctgcg cgccctgccc gacaaggcca aggcctggca ggacctttgc ctggcgctgg
     3121 aggtgatgcg cggcagcgcc ccgggctgaa gcgggctcgc tttgaaagac gaatgacctc
     3181 gttgcgcttc gatgacccat gggacatggg tcaagccgat gcagccgctg tcgttggtcc
     3241 gcttgttctt cgcgaagcca caggaggcgg ggccccggat ccccggatgg cgcgcggcgc
     3301 cagccggccg gtgtcggggg ccctcaggcg tcgatgttca gcgcccgcag cgcgttggtt
     3361 tcgatgaagg cgcggcgcgg ctcgacttcg tcgcccatca gggtggtgaa gacgcggtcg
     3421 gcttcgatgg cgtcttcgat ctgcacgcgc agcaggcgcc gcaccgccgg gtccatggtg
     3481 gtttcccaca gctgctccgg gttcatttcg cccaggccct tgtagcgctg gcgcgtggtg
     3541 gtgcgctcgg cctcgccgat cagccaggcc atggcctcgc ggaagctggc caccgcctgc
     3601 tcgcgctggc gctcgccttc gccgcggcgc acgcgcgcgc cttcgcccag caggtcgcgg
     3661 aaggtctgcg ccgcttcggc cagggcggcg tagtcggcgc cgtgcacgaa gtcctgcgtg
     3721 atgacgctgt ggcggacgtt gccgtggtgg cggcggctga tgcgcagcac cggtttgtcg
     3781 ctgcgggggt cgaattcgcc cgacacttcg ggcgcgctgc cgttggcctg ctcggcgtgc
     3841 aggtgctgct gcagcacggc ggccgaggcc tgggccgctt ccaccgtgtc caggttcagg
     3901 gtcacgccgg cggcgatggc gcgcagcgcc tcggtgtcca tgaaggccga cagacgctcg
     3961 atcacggctt cggccagctg gtggcggcgc gccagctcgt cgagggtgtc gccgctcagt
     4021 tgccgcgggg cggcgccgcc ggtgtgcacc acggcgtcct tcagcgccac gcgcagcagg
     4081 aaggcgtcca gcgcggcgcc gtccttcagg tacagctctt ccttgccgtt cttgaccttg
     4141 tacagcggcg gctgggcaat gtagatgtga ccgcgctcga ccagttcggg catctggcga
     4201 taaaagaacg tgagcagcag ggtgcggatg tgggcgccgt cgacgtcggc gtcggtcatg
     4261 atgatgacgc ggtggtagcg cagcttgtcg atgttgaagt cgtcggcgcc gctcttggtg
     4321 ctgccgttgc cgttgccgtc ttcggcgctg cggccgatgc cggtgcccag ggcggtgatg
     4381 agggtgacga tctcgttgct ggtgagcagt ttttcgtagc gcgcgcgctc gacgttcagg
     4441 atcttgccgc gcagcggcag gatggcctgg aacttgcggt cacgcccctg cttggcgctg
     4501 ccgccggcgc tgtcgccctc gacgatgtag atctcgcact gggccgggtc cttttcctgg
     4561 cagtcggcca gcttgccggg caggccgaag ccgtcgagca cgcccttgcg ccgcgtcatc
     4621 tcgcgcgcct tgcgggcggc ttcgcgggcg cgcgcggcct cgacgatctt gccgcagacg
     4681 atcttggcgt cgttggggcg ctcttccagg aattcggcca gcgccttgcc gacgatgtct
     4741 tcgaccggcg cgcgcacctc gctggacacc agcttgtcct tggtctggct gctgaacttg
     4801 ggctcgggca ccttgacgct gagcacgcac accaggcctt cgcgcaggtc gtcaccgctc
     4861 acatcgacct tggccttctt ggccagctcg ttgtcggcga tgtacttggt gatcacgcgc
     4921 gtcatggcgg cgcgcaggcc cgtgaggtgg gtgccgccgt cgcgctgcgg gatgttgttg
     4981 gtgaagcaca gcacct
//
LOCUS       DAOQTZ010000154         4974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_18738_length_4974_cov_15.703395, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000154 DAOQTZ010000000
VERSION     DAOQTZ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18738_length_4974_cov_15.703395"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKN51_12780"
     CDS             <1..297
                     /locus_tag="PKN51_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNT86018.1"
                     /translation="GALALGGALGTIAVIVRQEIVLAIMGGIFVVEALSVMLQVGWFK
                     YTKRRYGEGRRLLKMAPLHHHFEKNGWAETKVVVRFWIITMLLCLAGLSSLKLR"
     gene            301..1824
                     /gene="murD"
                     /locus_tag="PKN51_12785"
     CDS             301..1824
                     /gene="murD"
                     /locus_tag="PKN51_12785"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNT86019.1"
                     /translation="MNLLQDRHVLVLGLGLSGLAMARWCARQGASVTVADTRAEPPQL
                     AALRECLPAARFICGALDATLLEDASVRAVYRSPGLAPASIATLVQAARERGLPVGGE
                     LELFMQALQELGQARGYQPRLLAVTGTNGKTTVTALTGQLVERAGLSVAVAGNIGPSL
                     LDTLQARLDGEALPEVWVLELSSFQLDGVAGFEPTAATVLNVTQDHLDWHGDMASYAA
                     AKARIFGEHGVMVLNRNDPVVIGMRPAVPARSFKGPRPTPRRVVTFGADLPDAPGDFG
                     IEMVSGMAWLVRAQEADETQRRRKDAELELHLQRLMPADALRIRGRHNAVNALAALAL
                     ASSAGGALGPMLYGLREYRGEPHRVESVGVLEGVEYFDDSKGTNVGATVAALEGLGAE
                     RRLVVILGGDGKGQDFSPLAAPVSRFARAAVLIGRDAPQVHAALQQAGVELLSAEDMG
                     AAVALARSRAQAGDAVLLSPACASLDMYANYVARADAFRAAVREQALAAGVELEVLA"
     gene            1839..3086
                     /gene="ftsW"
                     /locus_tag="PKN51_12790"
     CDS             1839..3086
                     /gene="ftsW"
                     /locus_tag="PKN51_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNT86020.1"
                     /translation="MPSDGGKKRRAARARQMDELPVRIGGMPYARTTSTPTRVLGFDQ
                     SLIWVCVALLAWGLVMVYSASIALPDNPRFSRYAPGHFVLRHAFWIVLSFVAALIAFQ
                     VRMDWWEKVAPWLFVSALGLLIAVLIPHVGTVVNGARRWISLGPINFQPSELAKMAVL
                     LYAADYMVRKMDVKERFFRAVLPMGVAVGVVGLLLLAQPDMGAFLVIAVIAMGILFLG
                     GVNARMFFLIAAILVVAFAVMIWASPWRRERIFAYLDPFSEDHALGKGYQLSHALIAI
                     GRGEIFGVGLGGSIEKLHWLPEAHTDFLLAVIGEEFGLVGLVCVIFLFFWLTRRIMHI
                     GRQAIALDRVFSGLVAQGIGLWLGFQAFINIGVNLGALPTKGLTLPFMSYGGSAILAN
                     MVAIAIVLRVDVENRRLMHGGRA"
     gene            3083..4147
                     /gene="murG"
                     /locus_tag="PKN51_12795"
     CDS             3083..4147
                     /gene="murG"
                     /locus_tag="PKN51_12795"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNT86021.1"
                     /translation="MKQKCALVMAGGTGGHIFPGLAVADGLRARGWRVHWLGAPGSME
                     QQLVPPRGYPFEAIDFGGVRGKGLVTLALLPLRLLRAFAQSIGVIRRVRPDVLVGLGG
                     YITFPGGLMGVALGKPLVLHEQNSVAGMANRVLAGVADRVFTAFPQVLRKGRWIGNPL
                     RPEFAAQPAPAQRFAGRHGPLRVLVVGGSLGAQALNDVVPRALALLPPDARPQVRHQS
                     GARQIDALRANYAAAGVQAELLPFIDDMAQALAEADLVICRAGATTVTEIAAIGAAAL
                     FVPFPHAVDDHQTTNARFLVDQGGGWLVRQSELSPESLAQMLQNMERSALMERALKAK
                     TFQKTQAVEEMVAACEELAA"
     gene            4144..>4974
                     /locus_tag="PKN51_12800"
     CDS             4144..>4974
                     /locus_tag="PKN51_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNT86022.1"
                     /translation="MRHAIRHIHFVGIGGAGMSGIAEVLHNLGYVISGSDLADGAALH
                     RLSQLGIRTFVGHAAAHVEGADAVVTSTAVKPDNPEVLVARERRIPVVPRAIMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLNSAGANARLGSGDHIVVE
                     ADESDASFLNLLPVLAVVTNIDADHMETYGHDFGRLKRAFIDFLHRMPFYGTAILCLE
                     SPAVREIAGELSCPVTSYGFAADAQVRAVDVRAVGAQMHFTVQRRNGVQLPDLPVVLN
                     L"
BASE COUNT          655 a         1669 c         1837 g          813 t
ORIGIN      
        1 ggcgcgctgg cgctgggcgg ggcgctgggc accatcgccg tcatcgtgcg gcaggagatc
       61 gtgctggcca tcatgggcgg catcttcgtc gtcgaggcgc tgtcggtgat gctgcaggtg
      121 ggctggttca agtacaccaa gcggcgctac ggcgaaggcc ggcgcctgct caagatggcg
      181 ccgctgcacc accacttcga gaagaacggc tgggccgaga ccaaggtggt ggtgcgcttc
      241 tggatcatca ccatgctgct gtgcctggca ggtttgtcgt cgctcaagct gaggtagccg
      301 atgaacctgc tgcaagatcg tcacgtgctg gtgctgggcc tgggcctgtc gggcctggcc
      361 atggcccgct ggtgtgcccg tcagggcgcc tcggtcacgg tggccgacac gcgcgccgag
      421 ccgcctcagc tggccgcgct gcgcgagtgc ctgccggccg cccgcttcat ctgtggcgcg
      481 ctcgacgcca cgctgctcga agacgccagc gtgcgcgccg tctaccgcag tcccgggctg
      541 gcacctgcca gcatcgccac gctggtgcag gcggcgcgcg agcggggcct gccggtcggc
      601 ggcgagctgg agctgttcat gcaggcgctg caggagcttg ggcaggcgcg cggctaccag
      661 cccaggctgc tggcggtgac cggcaccaac ggcaagacca cggtgacggc gctcaccgga
      721 cagctggtcg agcgcgccgg cctgtcggtg gcggtggccg gcaacatcgg tcccagcctg
      781 ctcgacacgc tgcaggcgcg gctcgacggc gaggcgctgc ccgaggtctg ggtgctggag
      841 ctgtcgagct tccagctcga tggcgtggcc ggcttcgagc ccaccgcggc cacggtgctc
      901 aacgtcacgc aggaccatct ggactggcat ggcgacatgg cgagctacgc cgccgccaag
      961 gcgcgcatct tcggcgagca cggcgtgatg gtgctgaacc gcaacgatcc ggtggtgatc
     1021 ggcatgcggc cggcggtgcc ggccaggtcg ttcaagggcc cgcggccgac gccgcgccgc
     1081 gtcgtcacct tcggcgccga cctgcccgat gcgccgggcg acttcggcat cgagatggtg
     1141 tcgggcatgg cctggctggt gcgcgcgcag gaggccgacg agacccagcg ccggcgcaag
     1201 gacgccgagc tcgaactgca cctgcagcgc ctgatgccgg ccgacgcgct gcgcatccgc
     1261 ggtcgccaca atgccgtcaa cgcactggcc gcgctggccc tggccagcag cgccggcggc
     1321 gcgctcggcc ccatgctgta cggcctgcgc gagtaccggg gcgaaccgca ccgggtggag
     1381 tcggtcggtg tgctggaggg cgtcgagtac ttcgacgaca gcaaaggcac caacgtcggg
     1441 gccacggtgg cggcgctgga aggcctgggt gccgagcgcc ggctggtggt gattctgggc
     1501 ggcgacggca aggggcagga cttttctccc ctggcggcgc cggtgtcgcg cttcgcgcgc
     1561 gcggcggtgc tgatcgggcg cgacgcgccg caggtgcacg cggcgctgca gcaggccggg
     1621 gtggagctgc tgtcggccga ggacatgggt gccgccgtgg ccctggcgcg ctcgcgcgcc
     1681 caggcgggcg atgcggtgct gctgtcgccg gcctgcgcca gcctggacat gtacgccaac
     1741 tacgtggcgc gcgccgatgc cttccgcgcc gcggtgcgtg agcaggcgct ggcggcgggg
     1801 gtggagctgg aggtgctggc atgagcgcca ctgcgccgat gccgtcggac ggcggcaaga
     1861 agcggcgcgc cgcgcgcgcg cgccagatgg acgagctgcc ggtgcgcatc ggcggcatgc
     1921 cttatgcgcg caccaccagt acgcccacgc gcgtgctggg gttcgaccag tcgctgatct
     1981 gggtgtgcgt ggcgttgctg gcctggggtc tggtgatggt gtattcggcc agcatcgccc
     2041 tgcccgacaa cccgcgcttc agccggtatg cgccaggcca tttcgtgctg cggcacgcgt
     2101 tctggatcgt gctgtccttc gtcgcggccc tgatcgcctt tcaggtgcgc atggactggt
     2161 gggaaaaagt cgcgccctgg ctgttcgtca gcgcgctggg gctgctgatc gcggtgctga
     2221 tcccgcatgt cggcacggtg gtgaacggcg cgcgacgctg gatctcgctc ggtccgatca
     2281 atttccagcc gtccgagctg gccaagatgg ccgtgctgct gtacgcggcc gactacatgg
     2341 tgcgcaagat ggacgtcaag gagcgcttct tccgcgccgt gctgcccatg ggcgtggcgg
     2401 tcggcgtggt cgggctgctg ctgctggcgc agcccgacat gggcgccttc ctggtgatcg
     2461 cggtcatcgc catgggcatc ctgtttctgg gtggcgtcaa cgcccgcatg ttctttctca
     2521 tcgcggccat cctggtggtg gccttcgcgg tcatgatctg ggcttcgccc tggcggcgcg
     2581 agcgcatctt cgcctacctc gatccgttca gcgaggacca tgcactgggc aagggctacc
     2641 agttgtcgca cgcgctgatc gccatcgggc gcggcgagat cttcggtgtc ggtctggggg
     2701 gcagcatcga gaagctgcac tggctgcccg aagcgcacac cgactttctg ctggcggtga
     2761 ttggcgagga gttcgggctg gtcggcctgg tgtgcgtgat ctttctgttt ttctggctga
     2821 cccgccgcat catgcacatc ggccgccagg cgatcgcgct ggaccgcgtg ttctccggcc
     2881 tggtggcgca gggcatcggc ctgtggctcg ggttccaggc cttcatcaac atcggcgtca
     2941 acctgggtgc gttgccgacc aaggggttga cgctgccctt catgagctac ggcggttcgg
     3001 ccatcctggc caacatggtg gcgatcgcca tcgtgctgcg cgtggacgtg gaaaaccgtc
     3061 gcctgatgca tggaggccgc gcatgaagca gaagtgtgcg ctggtgatgg ccggcggcac
     3121 cggggggcac atcttcccgg ggctggcggt ggccgacggt ctgcgcgcgc gcggctggcg
     3181 cgtgcactgg ctgggcgcgc cgggcagcat ggagcagcaa ctggtgccgc cgcggggcta
     3241 cccgttcgag gcgatcgatt tcggcggcgt gcgcggcaag ggcctggtca cgctggcgct
     3301 gctgccgctg cgcctgctgc gggcgtttgc gcagagcatc ggcgtgatcc ggcgcgtgcg
     3361 gcccgacgtg ctggtgggcc tgggcggcta catcactttc ccgggcggcc tgatgggtgt
     3421 ggcactgggc aagccgctgg tgctgcacga gcagaattcg gtcgccggca tggccaaccg
     3481 ggtactggcg ggcgtggccg accgcgtctt cacggccttc ccgcaggtgc tgcgcaaggg
     3541 gcgctggatc ggcaacccgc tgcgcccgga gttcgccgcc cagcccgcac cggcgcagcg
     3601 ctttgccggt cgccacgggc cgctgcgtgt gctggtggtc ggcggcagcc tgggcgcgca
     3661 ggcgctcaac gacgtggtgc cgcgcgcatt ggccctgctg ccgcccgacg cgcgcccgca
     3721 ggtccgtcac cagagcggag cgcgccagat cgacgcattg cgtgccaact acgccgccgc
     3781 cggcgtgcag gccgagctgc tgcctttcat cgacgacatg gcgcaggccc tggccgaggc
     3841 cgatctggtg atctgccgcg ccggtgccac caccgtgact gagatcgccg ccatcggcgc
     3901 ggcggcgctg ttcgtgccgt ttccgcacgc ggtcgacgac catcagacca ccaacgcccg
     3961 cttcctggtc gatcaggggg gcggctggct ggtgcggcag agcgaactga gtcccgagtc
     4021 attggcccag atgctacaaa atatggagcg ttcggcgctg atggagcggg cgctgaaggc
     4081 caaaaccttc caaaagacgc aggcggtcga agagatggtc gcggcctgcg aggagctggc
     4141 tgcatgagac acgccatccg tcacatccac ttcgtcggca tcggcggcgc cggcatgagc
     4201 ggcattgccg aggtgctgca caacctgggc tacgtcatct ccggctccga cctggccgac
     4261 ggtgccgcgc tgcaccgcct gtcgcagctg ggcatccgca cgttcgtggg ccacgcggcc
     4321 gcgcatgtgg aaggggccga cgcggtcgtc acctccaccg ccgtcaagcc cgacaacccc
     4381 gaggtgctgg tggcgcgcga gcgccgcatc ccggtggtgc cgcgcgccat catgctggcc
     4441 gagctgatgc gcctgaagca gggcatcgcc atcgccggca cgcacggcaa gaccaccacc
     4501 accagcctgg tcgccagcgt gctggccgag ggtgggctgg accccacctt cgtgatcggc
     4561 gggcgcctga acagcgccgg cgccaacgcg cgcctgggct ccggggacca catcgtggtg
     4621 gaggccgatg aatccgacgc ttcgttcctg aacctgctgc cggtgctggc ggtcgtcacc
     4681 aacatcgacg ccgaccacat ggagacctac gggcacgact tcggccggct caagagggcg
     4741 ttcatcgact tcctgcaccg catgccgttc tacggcaccg ccatcctgtg cctggagagc
     4801 ccggcggtgc gcgagatcgc cggcgagctg agctgcccgg tcaccagcta cggcttcgcc
     4861 gccgacgccc aggtgcgcgc cgtcgacgtg cgcgccgtcg gcgcgcagat gcacttcacg
     4921 gtgcagcggc gcaacggcgt gcagttgccc gacctgccgg tggtgctcaa cctg
//
LOCUS       DAOQTZ010000155         4898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_19126_length_4898_cov_13.557712, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000155 DAOQTZ010000000
VERSION     DAOQTZ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19126_length_4898_cov_13.557712"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..630)
                     /locus_tag="PKN51_12805"
     CDS             complement(85..630)
                     /locus_tag="PKN51_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HNT86023.1"
                     /translation="MIRLNSFTTAAAVIAAGAALTACGSMSKGTATARASLAPTAAIT
                     PNPTSGSATFTALAHGVRVAGEVRGLAPGSEHGFHIHEKGDCGNNGNASGGHFNPAGG
                     MHGKFGGAGTHAGELPSLVADAAGVARFSVDVHSISLTDGAANNVVGRALVVHRDRDD
                     FSTQPAGNSGPRIACAVITRE"
     gene            complement(869..2542)
                     /locus_tag="PKN51_12810"
     CDS             complement(869..2542)
                     /locus_tag="PKN51_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT86024.1"
                     /translation="MPLPPPPAQRGARLSPDVIDLATRRAKTSAATPPSQPSSPARSA
                     PSARTAPPSGSPAPAALLQAPAPAPVSSTPKAPPARRPKSTPGARKARTQGPARLFVL
                     DTNVLLHDPMALFRFKEHDIFLPMIVLEELDGHKKGMSEVARNGRQTSRLLDALAGVR
                     GADMAQGLKLDATGHVEASGHLFFQTTPLDATMPAALPQGKADNQILGVVAALETQHA
                     PREVVLVSKDINMRVKARALGLNAEDYENDKTLDDGDLLYAGVLQLPTDFWTRQSKTV
                     ESWQSGSATFYRISGPVVPSLQINQFVFFEAPGEPSLYARVTEIQGKTAVLRTLKDYT
                     HLKNAVWGVTARNREQNFALNLLMDPEIDFVTLAGTAGTGKTLLALASGLTQVLDDRR
                     YTEIIMTRATVSVGEDIGFLPGTEEEKMGPWMGALDDNLEFLSKGDGSGAGEWGRAAT
                     NELIRSRVKIKSMNFMRGRTFMNKWIIIDEAQNLTPKQMKTLITRAGPGTKIICMGNL
                     AQIDTPYLTEGSSGLTYAVDRFKGWPHGGHVTLARGERSRLADFASEVL"
     gene            complement(2723..3199)
                     /locus_tag="PKN51_12815"
     CDS             complement(2723..3199)
                     /locus_tag="PKN51_12815"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNT86025.1"
                     /translation="MAIVVNKPLPEFEAMATGGVKVSNSSHLGQAVVLYFYPKDNTPG
                     CTTEAMQFRDKYKDFVKAGAIVFGVSRDNMASHDSFKTKLELPFELIADTEEKMCHMF
                     GVVKNKIMYGKKVKGIERSTFLINAEGIVVQEWRGLKVPGHVEDVLKAVKVLKKAA"
     gene            complement(3316..3720)
                     /locus_tag="PKN51_12820"
     CDS             complement(3316..3720)
                     /locus_tag="PKN51_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HNT86026.1"
                     /translation="MKFQPDQFGSAITSYGPGWIAIGGQRLEHSLVLRSDGEHALWNC
                     SRFDELTEAHFTLLVALQPQLVLFGSGARLRFPRPAWVRGLIEAGIGVETMDTGAACR
                     TYNILAGEGRRVVAALLVEAGTSGTSGTDRLE"
     gene            3800..>4898
                     /locus_tag="PKN51_12825"
     CDS             3800..>4898
                     /locus_tag="PKN51_12825"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT86027.1"
                     /translation="MKTIHKSTKLANVLYDVRGPIVDAARQMEDEGQKIIKLNIGNLA
                     PFGFEPPEEIVRDMIRNLPDSAGYSDSKGIFSARKAVMHYTQEQGIEGVTIDDIYLGN
                     GASDLIGMATNALLSEGDEMLVPAPDYPLWTAVASLSGGKPVHYRCDEDNGWMPNLAD
                     IRARITPATRAIVVINPNNPTGALYSDELLRGIVQIAREHGLVIMADEVYDKVLYDGV
                     RHTALASLSTDVLTLTFNSLSKAYRSCGFRAGWMVVSGPKDAARDYIEGINMLANIKL
                     GSNVPGQYAIQTALGGYQSIRDLVKEGGRLRRQRDLAYELITQIPGVSCVKPQAALYM
                     FPRLDPHIYPIDDDRQFFMEVLRATRVMLVQG"
BASE COUNT          746 a         1556 c         1737 g          859 t
ORIGIN      
        1 gtttcagtgg actaggatga ttgcaagcca aactggcact ttgcgcttgc tgggcaagcg
       61 ctggcagcta ttgaaagcgt agcactactc gcgcgtgatc accgcgcagg cgatgcgcgg
      121 gccggagttg ccggccggct gggtgctgaa gtcgtcgcga tcgcggtgca ccaccagcgc
      181 gcggccgacg acgttgttgg ccgcgccgtc ggtcagcgag atggagtgca cgtccacgct
      241 gaagcgcgcc acgccggcgg cgtcggccac caggctgggc agttcacccg cgtgggtgcc
      301 ggcgccgccg aacttgccat gcatgccgcc agcggggttg aagtggccgc ccgaggcgtt
      361 gccgttgttg ccgcagtcgc ccttttcgtg gatgtggaag ccgtgctcgc tgcccggcgc
      421 caggccgcgc acctcgccgg ccacgcgcac gccgtgcgcc agcgcggtga aggtcgcgct
      481 gccggaggtc gggttggggg tgatggcggc ggtgggtgcc aggctggcac gcgccgtggc
      541 ggtgcccttg ctcatgctgc cgcaggccgt gagtgcggcg ccggcggcga tgacggccgc
      601 ggccgtggtg aagctgttca ggcggatcat ttcggtgctc tccggtgggt gagggaaatg
      661 gctggcaggc cgtcgggctg gcttgcggat gctgaccggg catcataccc aggctcgccg
      721 ccgcgcgcag gtccgcggat gccgtttcgg tggccaagcg gaagcggggc gggtgcagca
      781 cccgcctccc gatcgctatt gaaagaatag ctgccagcgc ttgactggaa ggcgctggaa
      841 gtcgatttgg ccaaaaaccg gcgggcgctc agagcacctc gctggcgaag tcggccaggc
      901 gcgagcgctc gccgcgcgcc agggtgacgt ggccgccgtg cggccagccc ttgaagcggt
      961 cgacggcgta ggtcaggccg ctggagcctt cggtgaggta gggggtgtcg atctgcgcca
     1021 ggttgcccat gcagatgatc ttggtgccgg ggccagcgcg ggtgatcagg gtcttcatct
     1081 gcttgggcgt caggttctgc gcctcgtcga tgatgatcca cttgttcatg aaggtgcgcc
     1141 cgcgcatgaa gttcatgctc ttgatcttga cgcggctgcg gatcagttcg ttggtggcgg
     1201 cgcgtcccca ttcgcccgcg ccgctgccgt cgcccttgga caggaactcc aggttgtcgt
     1261 ccagcgcgcc catccagggg cccatttttt cctcttcggt gccgggcaga aagccgatgt
     1321 cctcacccac gctgacggtg gcgcgggtca tgatgatctc ggtgtagcgc cggtcgtcga
     1381 gcacctgcgt caggcccgag gccagcgcca gcagcgtctt gccggtgccg gccgtgccgg
     1441 ccagggtgac gaagtcgatc tccgggtcca tcagcaggtt gagcgcgaag ttctgctcgc
     1501 ggttgcgcgc cgtcacgccc cagacggcgt tcttcaggtg ggtgtagtcc ttgagtgtgc
     1561 gcagcaccgc ggtcttgccc tggatctcgg tgacgcgggc gtacaggctg ggctcgcccg
     1621 gggcctcgaa gaagacgaac tgattgatct gcaggctggg caccaccggc ccgctgatgc
     1681 ggtagaaggt ggcgctgccc gactgccagc tctcgaccgt cttgctctgg cgcgtccaga
     1741 agtcggtggg cagctgtagc acgccggcgt acagcaggtc accgtcgtcc agcgtcttgt
     1801 cgttctcgta gtcctcggcg ttcagcccca gcgcgcgcgc cttgacgcgc atgttgatgt
     1861 ccttggacac cagcaccact tcacgcgggg catgctgtgt ctccagcgcg gccaccacgc
     1921 ccaggatctg gttgtcggcc ttgccctggg gcagggcggc gggcatggtg gcgtccagcg
     1981 gcgtggtctg aaagaacagg tggccgctgg cttcgacatg gccggtggca tcgagcttta
     2041 gcccttgcgc catatcggcg ccccggacgc cggccagcgc gtccagcagg cggctggtct
     2101 ggcgcccgtt gcgcgccact tccgacatgc ccttcttgtg cccatcgagc tcctccagca
     2161 cgatcatcgg caggaagatg tcgtgctcct tgaagcgaaa cagcgccatc gggtcgtgca
     2221 gcagcacatt ggtgtcgagc acgaacaggc gcgccggccc ctgggtgcgg gccttgcgtg
     2281 cgcctggcgt cgatttgggg cgccgcgccg gcggcgcctt gggcgtcgag ctgacgggtg
     2341 ccggggccgg tgcctgcagc agggccgccg gggcagggga gcccgatggt ggcgccgtcc
     2401 gggccgaggg cgcagaccgt gcgggcgacg acggctggga cggcggggtg gccgcgctgg
     2461 tcttggcacg gcgtgtcgcc aggtcgatca cgtcgggaga caggcgggcg ccgcgttggg
     2521 caggaggggg aggcaggggc atgcttgtcg gctcagcgag caggggcggt gccggccagc
     2581 accggtgggc tggatggccg gcgggccaaa gaaaaagccg cctgggctgc cccggcggct
     2641 ttcagcgaga ggaacggtcg gtgcgtgaac aaccacattg cggcccatta tgcatgagcc
     2701 ggtgcggtgg ccggcaggcg cgtcaggcgg ccttcttgag gactttgacc gccttgagga
     2761 cgtcttccac gtggcccggc accttcaggc cgcgccattc ctgcaccacg atgccttcgg
     2821 cgttgatcag gaaggtgctg cgctcgatgc ccttgacctt cttgccgtac atgatcttgt
     2881 tcttgaccac gccgaacatg tggcacatct tttcttcggt atcggcgatc agctcgaacg
     2941 gcagttccag cttggtcttg aagctgtcgt gcgacgccat gttgtcgcgg ctgacgccga
     3001 agacgatggc gccggccttg acgaagtcct tgtacttgtc gcgaaactgc atggcctcgg
     3061 tggtgcagcc gggcgtgttg tccttggggt agaagtacag aaccaccgcc tgtccgaggt
     3121 gggagctgtt gctgaccttg acgccgccgg ttgccatcgc ttcgaattca ggaaggggtt
     3181 tgttgacaac gatcgccata tgaacttcaa tctccggacg gtgttatggg tgacgcggac
     3241 cacgcagcgc ccatgcacag actgctgctg cctcgactgc gcggcgtcgg gtgcggggcc
     3301 tcggtggcaa gcccgtcatt ctaaccgatc ggtcccgctc gtgccgctcg tgccggcttc
     3361 caccagcagt gccgccacca cgcggcggcc ttcgccggcc agaatgttgt aggtgcggca
     3421 ggcggcgccg gtgtccatgg tttccacccc gatgcccgct tcgatcaggc cccgcaccca
     3481 ggccgggcgc ggaaagcgca ggcgggcacc actgccgaac agcaccagtt gcggctgcag
     3541 cgcgaccagg agggtgaaat gcgcctccgt cagttcgtca aaacggctgc agttccagag
     3601 cgcgtgttcg ccatcgctgc gcagcaccag gctgtgttcc agacgctggc cgccgatggc
     3661 gatccagccg gggccatagc tggtgatggc ggagccgaac tggtcgggct ggaatttcat
     3721 ggcgggcggc gtgggcggtg gagcgaatgt gttccaattc taggtttccc ccgggatgac
     3781 tgccgcgcga gagggtttca tgaagacgat ccacaagtcg accaagctgg ccaacgtgct
     3841 gtacgacgtg cgcgggccga tcgtggacgc cgcccgccag atggaggacg agggtcagaa
     3901 gatcatcaag ctgaacatcg gcaacctggc gccgttcggc ttcgagccgc ccgaagagat
     3961 cgtgcgcgac atgatccgca atctgccgga ctcggccggt tactccgaca gcaagggcat
     4021 tttttccgcg cgcaaggcgg tgatgcacta cacgcaggag cagggcatcg aaggcgtcac
     4081 catcgacgac atctacctgg gcaacggcgc cagcgacctg atcggcatgg ccaccaacgc
     4141 cctgctgtcc gagggcgatg aaatgctggt gccggcgccc gactacccgc tgtggacggc
     4201 ggtggccagc ctctcgggcg gcaagccggt gcactaccgc tgcgacgagg acaacggctg
     4261 gatgccgaac ctggccgaca tccgcgccag gatcacgccg gccacccgcg ccatcgtcgt
     4321 catcaacccc aacaacccga ccggggcgct gtattccgac gaactgctgc gcggcatcgt
     4381 gcagatcgcg cgcgagcacg gcctggtcat catggccgac gaggtgtacg acaaggtgct
     4441 gtacgacggc gtgcgccaca cggcgctggc cagcctgtcg accgatgtgc tgacgctgac
     4501 cttcaactcg ctgtccaagg cctaccgctc ctgcggcttt cgcgccggct ggatggtggt
     4561 gtccggcccc aaggacgcag cgcgcgacta catcgagggc atcaacatgc tggccaacat
     4621 caagctgggc agcaacgtgc cggggcagta tgcgatccag accgcgctgg gcggctacca
     4681 gagcatccgg gatctggtga aggaaggcgg gcgcctgcgc cgccagcgcg acctggccta
     4741 cgagctgatc acgcagatcc ccggggtcag ctgcgtcaag ccgcaggcgg cgctgtacat
     4801 gttcccgcgg ctggatccgc acatctaccc gatcgacgac gatcgccagt tcttcatgga
     4861 ggtgctgcgc gccacgcgcg tgatgctggt gcagggct
//
LOCUS       DAOQTZ010000156         4851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_19385_length_4851_cov_14.224354, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000156 DAOQTZ010000000
VERSION     DAOQTZ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19385_length_4851_cov_14.224354"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKN51_12830"
     CDS             <1..573
                     /locus_tag="PKN51_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNT86028.1"
                     /translation="TLAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFA
                     HLDSTVVLSRDIAALGIYPAVDPLDSTSRQLDPHVVGEEHYNVARAVQGTLQRYKELR
                     DIIAILGMDELSPEDKLAVARARKIQRFLSQPFHVAEVFTGSPGKYVPLKDTIHGFKM
                     IVNGECDHLPEQAFYMVGTIEEAMEKAKKV"
     gene            667..1086
                     /locus_tag="PKN51_12835"
     CDS             667..1086
                     /locus_tag="PKN51_12835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNT86029.1"
                     /translation="MANTIHVDVVSAEESIFSGEATFVALPGEAGELGIYPRHTPLIT
                     RIKPGSVRIQLPGGGEEFVFVAGGILEVQPDVITVLSDTAIRGKDLDEAKASEARKQA
                     EEALKNAKSEIDMARATSELAVLAAQIAALRKFRQKR"
     gene            1201..1797
                     /locus_tag="PKN51_12840"
     CDS             1201..1797
                     /locus_tag="PKN51_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT86030.1"
                     /translation="MMPDQERSDQSASLRAAALLVTEGALVELSAADAREVVSYMRPQ
                     RVGAGQIVIRAGETRRNDFMALVLHGEVTVENAPAATHEGMVVSVLGPGSLIGEMGLV
                     DGGPRSATCTANSDLVLAVLTREAVLHMMDARPAVAARLMLAIAKRIADHLRDTNRKL
                     MTLARVSKALQQELDAVHAVNKRLLDQVPATPVAKARG"
     gene            complement(1861..2400)
                     /locus_tag="PKN51_12845"
     CDS             complement(1861..2400)
                     /locus_tag="PKN51_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNT86031.1"
                     /translation="MWTTLTRLGRHLWLDRSHTARAIPAATAERLRQRIAASEQRHSG
                     EVRICVEASLPASYLWRHLRQRLPIRQIVRQRALMMFSKLRVWDTEHNNGVLIYLLLA
                     ERAIELVADRGVMRVVEPTAWHEAVAHLGAAWHDGRFEEGLTQALEEVSAMLVTHFPN
                     SGERQGPAPHQLPDEPVLR"
     gene            complement(2404..3333)
                     /locus_tag="PKN51_12850"
     CDS             complement(2404..3333)
                     /locus_tag="PKN51_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNT86032.1"
                     /translation="MRLASVLKALIAIVLVAIGTAAALAQPLQPVPALTAHVIDTTGT
                     LDAARQAALEARLAALEQDKGAQVVVLLVQTTAPEDIAAYANRVGNSWKIGRRDVGDG
                     LIVLVAVQDRRMRIEVAKTLEGAVPDIAARQIIDEAMAPHFRRGDYAGGLDAAVEQLG
                     ARIRGEPLPPVRTTGAGTLQNELDFSLFEMLVLLFIIVPIIGAVARGVLGRKLGALAT
                     GAGVGWLAFVVTASWLLALVAAALSLFIALLGGVSGALPSPRSRGGPWGGGFGGGGLG
                     GGGFGGGSFGSGGGGFSSGGGGDFGGGGASGSW"
     gene            complement(3371..3991)
                     /locus_tag="PKN51_12855"
     CDS             complement(3371..3991)
                     /locus_tag="PKN51_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNT86033.1"
                     /translation="MHMTRWFIKMAAWLAVALSLSGCGYNDFQRLDETTKSAWSEVVN
                     QYQRRADLIDNLVATVKGEANFEQETLTKVIEARAKATSIQVTPETLNNPEAFKQFQA
                     AQGELSSALSRLMVTVERYPDLKANKAFAELRVALEGTENRIAVARGRYIQAVQEYNV
                     LARSFPTNLTAMVFGYHAKENFSVTNEAAISTAPRVQFGPATAASR"
     gene            4142..4588
                     /locus_tag="PKN51_12860"
     CDS             4142..4588
                     /locus_tag="PKN51_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNT86034.1"
                     /translation="MPRTRYHAAQFGGSADEVEVAFYEALQQGDLEQLMACWADEDDI
                     VCVHPGGPRLVGAAAIRQAFELMFSQGTIRATPERLRRVETLGAAVHNVLERVDQHSA
                     EGTRRAWVVATNVYLKTTQGWRLVLHHASPGRSEVAEVSEPAPLLH"
BASE COUNT          793 a         1690 c         1633 g          735 t
ORIGIN      
        1 acgctggccg aggaaatggg tcgcctgcag gagcgcatca cctccaccaa ggtgggctcg
       61 atcacctcga tccaggccgt gtacgtgcct gccgacgacc tgaccgaccc ctcgcccgcc
      121 acgaccttcg cccacctgga ctccaccgtg gtgctgtcgc gtgacatcgc ggccctgggc
      181 atctatccgg cggtggaccc gctggactcc accagccgcc agctcgaccc ccatgtggtg
      241 ggcgaggagc actacaacgt cgcccgcgcc gtgcagggca cgctgcagcg ctacaaggaa
      301 ctgcgcgaca tcatcgccat tctgggcatg gacgagctct cgcccgagga caagctggcc
      361 gtggcacgcg cgcgcaagat ccagcgcttc ctgtcgcagc cgttccacgt ggccgaggtg
      421 ttcaccggct cgcccggcaa gtacgtgccg ctgaaggaca ccatccacgg cttcaagatg
      481 atcgtcaatg gcgagtgcga ccacctgcca gaacaggcct tctacatggt cggcaccatc
      541 gaagaagcga tggaaaaggc caagaaagtc tgaacttcgg gttcggcgtc cggcgttacg
      601 tgctgagcgc ggcggtccga aggatcggcc catcgttcag cgcgcaacgc ccaaccagga
      661 gaaagcatgg ccaataccat tcacgtggac gtggtgagcg ccgaggagtc gatcttctcg
      721 ggcgaggcca cgttcgtggc gctgcccggc gaggcgggcg agctgggcat ctacccgcgc
      781 cacacgccgc tgatcacgcg catcaagccg ggctcggtgc gcattcagct gcccggcggc
      841 ggcgaggaat tcgtctttgt cgcgggcggc attctggaag tgcagcccga cgtcatcacg
      901 gtgctgtccg acaccgccat tcgcggcaag gacctggacg aagccaaggc cagcgaggca
      961 cgcaagcagg ccgaagaagc gctgaagaac gccaagagcg agatcgacat ggcgcgcgcc
     1021 acgtccgagc tggcggtgct ggcggcgcag atcgccgcgc tgcgcaagtt ccggcaaaag
     1081 cgctgaacgg cagcttgatc gccgccaacc ggcatttgtc cacaagcccg ccttcgagcg
     1141 ggctttttgt tgcggcgtgc ggcgccctcg cgtgcgtgtc gtccctctaa aatcctcccc
     1201 atgatgcctg accaggaacg ctccgaccag agcgcctcgc tgcgcgccgc ggccttgctc
     1261 gtcaccgagg gggcgctggt cgagctcagc gccgccgatg cgcgcgaggt ggtcagctac
     1321 atgcgaccac aacgcgtggg tgcggggcag atcgtcatcc gcgccggcga gacccggcgc
     1381 aacgacttca tggcgctggt gctccacggc gaggtgacgg tcgagaacgc ccccgccgca
     1441 acccacgaag gcatggtggt gtcggtgctg ggcccgggca gcctgattgg cgaaatgggt
     1501 ctggtggatg gcgggccgcg ctcggccacc tgcaccgcca actcggatct ggtgctggcg
     1561 gtgctgacgc gcgaagccgt gctgcacatg atggacgcgc gccccgcggt ggcggcacga
     1621 ctgatgctgg cgatcgccaa gcgcattgcc gaccacctgc gcgacaccaa ccgcaagctg
     1681 atgaccctgg cgcgggtgag caaggcgctg cagcaggagc tcgacgccgt gcacgcggtc
     1741 aacaagcgcc tgctggatca ggtgccggcg acgccggtgg ccaaggcccg cggctgagcg
     1801 ccggcgactg cgcctgttga ttcggccttt ggatgcccac aggcttccga agcagtaggc
     1861 tcagcgcagc accggctcgt ctggcagctg atgcggcgcc gggccctggc gctcgcccga
     1921 gttggggaag tgcgtcacca gcatggccga cacttcttcc agcgcctgcg tcaacccctc
     1981 ttcgaagcgg ccgtcgtgcc acgccgcgcc caggtgggcc accgcctcgt gccaggccgt
     2041 cggctccacc acccgcatca cgccgcggtc ggccaccagc tcgatcgccc gctcggccag
     2101 cagcaggtag atcagcacgc cattgttgtg ctcggtgtcc cacacgcgca gcttggaaaa
     2161 catcatcagc gcgcgctggc gcacgatctg cctgatcggc aggcgctggc gcaggtgacg
     2221 ccacaggtag ctggccggca ggctggcctc gacgcagatg cgcacctcac cgctgtggcg
     2281 ttgctcgctg gcagcgatgc gctggcgcag cctttcggcc gtggcggccg ggatggcgcg
     2341 cgcggtgtgg gaccgatcca gccagaggtg gcgccccagg cgcgtcaagg tggtccacat
     2401 ggtctaccag ctcccactgg cgccgccgcc accaaaatcg ccgccgccgc ctgaactgaa
     2461 gcctccgccg ccggagccga aactcccgcc accgaagccc ccgcccccaa gccctccgcc
     2521 gccgaagccg ccaccccagg ggccaccgcg cgagcgcggc gaaggcagcg caccactcac
     2581 accgcccagc agcgcgatga acagcgagag cgccgcggcc accagcgcca gcagccagct
     2641 ggcggtgacc acgaaggcca gccagcccac cccggcgccg gtggccaggg cgcccagctt
     2701 gcgaccgagc acgccgcgcg ccacggcgcc gatgatcggc acgatgatga acagcagcac
     2761 cagcatctcg aacaggctga agtccagctc gttctgcagt gtgccggcac ccgtggtgcg
     2821 cacgggcggc agcggttcac cgcggatgcg ggcgcccagt tgctcgacgg cggcgtccag
     2881 cccgccggca tagtcgccgc gccgaaaatg gggcgccatg gcctcgtcga tgatctgacg
     2941 cgcggcgatg tcgggcaccg ccccttccag cgtcttggcg acttcgatgc gcatgcgccg
     3001 gtcctgcacc gccaccagca cgatcaggcc gtcgcccacg tcgcggcggc cgatcttcca
     3061 gctgttgccg acgcggttgg cgtaggcggc gatgtcttcc ggcgccgtgg tctgcaccaa
     3121 cagcaccacc acctgcgcgc ccttgtcctg ctccagcgcg gccagccgtg cttccagcgc
     3181 cgcctggcgg gcggcatcga gcgtgcccgt ggtgtcgatc acgtgcgccg tcagcgccgg
     3241 taccggctgc agcggctgag ccagcgccgc agcggtgccg atagctacca aaacgatagc
     3301 tatcagcgct ttcaggacgg acgccaggcg cactttttgc agtgaacgaa gggggcgagg
     3361 agccgccggt tcagcgcgat gccgccgtcg ccggaccgaa ctgcacgcgc ggcgcggtgg
     3421 agatggcggc ctcgttggtg acgctgaagt tttccttggc gtggtagccg aacaccatgg
     3481 cggtcaggtt ggtcgggaag ctgcgtgcca gcacgttgta ttcctgcacc gcctggatgt
     3541 agcggccgcg cgccacggcg atgcggtttt cggtgccttc cagcgccacg cgcagttcgg
     3601 cgaaggcctt gttggccttc aggtcggggt agcgttccac cgtcaccatc aggcggctga
     3661 gcgcgctgga cagctcgcct tgcgcggcct ggaactgctt gaacgcctcg gggttgttga
     3721 gcgtctcggg cgtgacctgg atcgaggtcg ccttggcgcg cgcctcgatc accttggtca
     3781 gggtttcctg ctcgaagttg gcctcgccct tgacggtggc caccaggttg tcgatcaggt
     3841 cggcgcggcg ctggtactga ttgaccacct cgctccaggc cgacttggtg gtttcgtcca
     3901 gccgttggaa atcgttgtag ccgcagccac tcagcgacag cgccaccgcc agccaggcgg
     3961 ccatcttgat gaaccagcga gtcatgtgca tgtgctctcc tgcaaaggcg cacgggacgc
     4021 gcgccacaat cgcaagcata tatggacacg cgccgccgca gtgcaagggg tgacgtggcc
     4081 ggggcggtgc gcggcacaat gcacaccttg gtctccgtca cgcctcatct tagcccgcgc
     4141 catgcccagg acccgctacc acgccgccca gttcggcggc tccgccgacg aagtcgaagt
     4201 tgcgttctac gaggcgttgc aacagggcga cctggagcaa ttgatggcct gctgggccga
     4261 cgaggacgac atcgtctgcg tccacccggg tggtccgcgc ctggtgggcg cggcggcgat
     4321 ccgccaggcc ttcgagctga tgttcagcca gggcacgatc cgcgccacgc ccgagcgcct
     4381 gcgccgcgtc gaaaccctgg gcgcggccgt gcacaacgtg ctggagcggg tggatcagca
     4441 cagcgccgaa ggcacgcgcc gcgcctgggt ggtggccacc aacgtctacc tcaagaccac
     4501 ccagggctgg cgcctggtgc tgcaccatgc cagcccgggg cgcagcgaag tggccgaagt
     4561 ctccgagccg gcgccacttc tgcattgagc ccgaagccgt tcgtgttctc gccagtggca
     4621 ggatgaattg gcccgtaggt ggccacaggc ttgcgccggc agctatgcta tggatagctt
     4681 atcgagggtg ccgccgcacg ccgggcacac ggcgagcgcg gtgcgggtgg gtgcgtagcg
     4741 cgctcaccca agaggtgaag gccttcggag ccgccaaaac ctgcatggac gcggcctgcc
     4801 cgaagatgac aagcggtcaa cggccttctc tgggttcgaa ggccgcttca a
//
LOCUS       DAOQTZ010000157         4825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_19520_length_4825_cov_12.661426, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000157 DAOQTZ010000000
VERSION     DAOQTZ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19520_length_4825_cov_12.661426"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..104
                     /locus_tag="PKN51_12865"
     tRNA            28..104
                     /locus_tag="PKN51_12865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62..64,aa:Arg,seq:acg)
     gene            169..810
                     /locus_tag="PKN51_12870"
     CDS             169..810
                     /locus_tag="PKN51_12870"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNT86035.1"
                     /translation="MFTGIITAVGRIAEVRELGASSAHGKQLVIETPPGYLDDVGLGD
                     SIALNGACMTATAIDARAGRFAIDISAESLARTAGLDRPGPVNLEKALRAHDRLGGHI
                     VSGHVDGIGQVTHFAPVGESWALCVRAPRELARYLAYKGSVAVNGVSLTVNQVDDQPG
                     GCEIHINLIPHTVQHTALHTLTAGSAVNLEIDLIARYVERMLSSAHPASAASS"
     gene            complement(807..1721)
                     /locus_tag="PKN51_12875"
     CDS             complement(807..1721)
                     /locus_tag="PKN51_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT86036.1"
                     /translation="MTALAASRPATSPARLTLYYLLLAATWGASFLFMRQATLEFGPL
                     PTAAVRVTVAALFLLPLLLAHGQWPALRRHWRPVLLCGLLNSGIPFALFSFALLHIST
                     GLSSILNATVPLFGALVAWAWLGQRPGISRGIGLVVGFVGVALLAWDKIGLRAGAPAA
                     LAAWAVAACLGATLCYALAASFTHRHLSGLPPLMTATGSQIGAMLGLALPALALWPAQ
                     MPGPTAWASVVALGVLCTGVAYVLYFRLIEALGPARALTVTFTVPVFAIVYGAALLGE
                     AVTPWMLLCGAIVLVGTALATGFIKLRF"
     gene            1891..3333
                     /locus_tag="PKN51_12880"
     CDS             1891..3333
                     /locus_tag="PKN51_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNT86037.1"
                     /translation="MQLHRAIFKAYDIRGTTPTTLNEAVAERLGLAFGQRALALGERV
                     VAVGRDGRLSGPALAAALMRGLVAAGVEVIDIGLATTPMLYFAASTLCRSGIQVTGSH
                     NPRDDNGFKMVLAGRAIYGEEIQALRRVMEAGEGARRAGGSVRQHDVFPAYLARIQGD
                     IRLARPMKIVVDCGNGVAGASAPQVLRAIGCEVMELFTEVDGRFPNHHPDPSKPDNLR
                     DVIAALRASDTELGLAFDGDGDRLGIVTRDGQNIFPDRQLVLFAQDVLTRVPGAPIVF
                     DVKCSQRLAPAIRAAGGVPLMHQTGHSLIKARMKEVGAPLGGEMSGHIFFQERWFGFD
                     DGTYAGCRLLEILSRSPDASAVLNALPTSCSTPELNVPCAEGEAHRLAAELQALAPVV
                     FAEPLSARPEGSEPRAAGSAGAAAISTIDGLRVDWPDGFGLIRASNTTPVLVLRFEGQ
                     TPQALARIQADMLALLRRVKPDAQLGAAAH"
     gene            3398..4732
                     /locus_tag="PKN51_12885"
     CDS             3398..4732
                     /locus_tag="PKN51_12885"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNT86038.1"
                     /translation="MKSLVGALYDLATWLAQPLLRRKLRRRGAAEPGYLHAVEERFGH
                     YTQARPVGAAPLVWVHAVSLGETRAAAILLAALRPRLPGMRLLLTHGTATGRAEGGRL
                     LQAGDVQVWQPWDSPGAVRRFLAHWRPDVGVLMETEIWPNLIREAAARGVPLALANAR
                     LSDRSLRGARRAGALLRPAYRALSAVWAQTEADAARLRALGAGPVTVLGNLKFDAQPA
                     PELLARGRAWRAGSDRPVVMLASSRDGEEAEFLKQIKALELSGQAPEAIENVATEAAA
                     PAVQWLIVPRHPQRFDDVAAAVAAAGLSLSRRSAWHALPEAAEVWLGDSLGEMAAYYA
                     LADVALLGGSFAPLGGQNLIEAAACGCPVLMGPHTFNFAQAAELAQAAGAARRVADLA
                     QAVHAARALLADAPARAAMRAAAQDFAQAHRGAAARSAEALQALLTGAEPAA"
BASE COUNT          708 a         1751 c         1742 g          624 t
ORIGIN      
        1 tggtcgtgat ataatttttt gcttcgcgcg gctgtagctc agttggatag agtacttggc
       61 tacgaaccaa ggggtcgtgg gttcgaatcc tgccagccgc gccaacccgt accgaaagcc
      121 ccgcaaatcc atggtttgcg gggctttctt cttttggggt ccgattgcat gttcaccggc
      181 atcatcaccg ccgtcggccg cattgccgag gtgcgcgaac tgggcgccag cagcgcgcat
      241 ggcaaacagc tcgtcatcga gacgccgccc ggctacctgg acgacgtggg cctgggcgac
      301 agcatcgcgc tcaacggcgc ctgcatgacg gccaccgcca tcgacgcgcg ggcggggcgc
      361 tttgccatcg acatctcggc cgaatcgctg gcgcgcaccg ccgggctcga tcggcccggc
      421 ccggtcaacc tggaaaaggc gctgcgcgcg cacgaccggc tgggcgggca catcgtgtcg
      481 gggcatgtgg acggcatcgg ccaggtcacg cacttcgcgc cggtgggcga gagctgggcg
      541 ctgtgcgtgc gggcgccgcg cgagctggcg cgctatctgg cctacaaggg ctcggtcgcc
      601 gtcaacggcg tgagcctgac ggtcaatcag gtggacgacc agccgggcgg ctgcgagatc
      661 cacatcaacc tgatcccaca caccgtgcag cacaccgcgc tgcacacgct gacggccggc
      721 agcgccgtca acctggagat cgacctgatc gcccgctatg tggagcggat gctttccagc
      781 gcccacccgg caagcgctgc aagctcctaa aaacgtagct tgatgaagcc cgtggcgagc
      841 gccgtgccga ccagcacgat ggcgccgcac agcagcatcc agggtgtcac ggcttcgccc
      901 agcagggcgg cgccatagac gatggcgaac accggcacgg tgaaggtcac cgtcagcgcg
      961 cgcgccgggc ccagcgcctc gatcaggcga aagtacagca cgtaggccac gccggtgcac
     1021 agcacgccca gcgccaccac cgaggcccag gccgtgggcc cgggcatctg cgccggccac
     1081 agcgccagcg ccggcagcgc caggcccagc atggcgccga tctggctgcc ggtggcggtc
     1141 atcagcggcg gcaggccgct caggtggcgg tgcgtgaagc tggccgccag cgcataacac
     1201 agcgtggcgc ccaggcaggc ggccacggcc caggcggcca gtgccgccgg tgcgccggcg
     1261 cgcaggccga tcttgtccca ggccagcagg gccacgccga caaagcccac caccagtccg
     1321 atgccgcgcg agatgccggg ccgttgcccc agccaggccc aggccaccag ggcgccgaac
     1381 agtggcacgg tggcgttgag gatcgacgac aggccggtgg agatgtgcag cagcgcaaag
     1441 ctgaacagcg caaacggaat gccggaattg agcagcccgc acagcagcac cggccgccag
     1501 tggcgccgca gcgccggcca ctggccatgc gccagcagca gcggcagcag aaacagcgcc
     1561 gccaccgtca cgcgcaccgc ggcggtcggc agtggtccga attcgagcgt ggcctggcgc
     1621 atgaacagga acgaggcccc ccaggtggcg gccagcagca ggtagtacag cgtcagccgg
     1681 gcggggctgg tggccgggcg ggaggcggcg agggcggtca tggcgcgcga gtatcgccgc
     1741 tgggtcaaac ccggcgccgg gcgcggggca aggcggtcgg cctggcggct gggctacgga
     1801 tctggaatga aatcggactc tggtgcagta cagtcaagcg ccggcagcta ttgaatcaat
     1861 agcatgtgcg gctccctaca atcaccgctc atgcagctcc atcgcgccat cttcaaggct
     1921 tacgacatcc gcggcaccac gccgaccacg ctgaacgagg ccgtcgccga gcgtctgggc
     1981 ctggccttcg gtcagcgcgc gctggcgctg ggcgagcgcg tggtggcggt ggggcgcgac
     2041 ggccgcctgt ccggtccggc gctggcggcg gcgctgatgc gcggcctggt ggctgccggc
     2101 gtcgaggtga tcgacatcgg cctggccacc acgccgatgc tgtactttgc cgccagcacg
     2161 ctgtgccgca gtggcatcca ggtcaccggc agccacaacc cgcgcgacga caacggcttc
     2221 aagatggtgc tggccgggcg cgccatctac ggcgaagaga tccaggccct gcgccgcgtg
     2281 atggaggcgg gcgagggcgc gcgccgcgcc ggcggctcgg tgcgccagca cgacgtgttc
     2341 ccggcgtacc tggcgcgcat ccagggcgac atccggctgg cacggccgat gaagatcgtg
     2401 gtcgactgcg gcaacggcgt ggccggcgcc agcgcgccgc aggtgctgcg cgccatcggc
     2461 tgcgaggtga tggagctgtt caccgaggtc gacggccgct ttccgaacca ccaccccgac
     2521 cccagcaaac ccgacaacct gcgcgacgtc atcgccgcgc tgcgcgcgtc cgacaccgaa
     2581 ctgggcctgg ccttcgacgg cgacggcgac cgcctgggca tcgtcacccg cgacggccag
     2641 aacatcttcc ccgaccgcca gctggtgctg ttcgcgcagg acgtgctgac ccgcgtgccg
     2701 ggcgcgccca tcgtgttcga cgtcaagtgc tcgcagcgcc tggcgccggc catccgcgcc
     2761 gccggcggcg tgccgctgat gcaccagacc ggccattcgc tgatcaaggc gcgcatgaag
     2821 gaggtcggcg cgcccctggg cggcgagatg agcggccaca tcttctttca ggagcgctgg
     2881 ttcggcttcg acgacggtac ctacgccggc tgccggctgc tggaaatcct cagccgctcg
     2941 cccgacgcca gcgccgtgct caatgccctg cccaccagtt gcagcacgcc cgagctgaac
     3001 gtgccctgcg ccgaaggcga ggcgcaccgt ctggcggccg agctgcaggc gctggcgccg
     3061 gtggtgtttg ccgagcccct gagcgcccgc ccggagggca gcgaaccacg cgcagcgggc
     3121 agcgcggggg cggctgcgat ctcgaccatc gacggcctgc gcgtcgactg gccggacggc
     3181 ttcggcctga tccgcgccag caacaccacg ccggtgctgg tgctgcgctt cgagggccag
     3241 acgccgcagg cgctggcgcg catccaggcc gacatgctgg cgctgctgcg gcgcgtcaag
     3301 cccgacgccc agctcggcgc cgctgcccat tgatgggcgc gtttgacggc cccagcgccc
     3361 acccggcgtg cgccagaagc tacacaaacc atagcgcatg aagtcgctcg tcggcgccct
     3421 ctacgacctg gccacctggc tggcccagcc cctgctgcgg cgcaagctgc gccgccgcgg
     3481 cgccgccgag ccgggctacc tgcatgccgt ggaggagcgc ttcggccact acacccaggc
     3541 ccgcccggtg ggcgccgcgc cgctggtgtg ggtgcacgcg gtgtcgctgg gcgagacgcg
     3601 cgctgccgcc attctgctgg cggcgctgcg cccacgcctg cccggcatgc gcctgctgct
     3661 cacgcatggc accgccaccg gacgcgccga gggaggcagg ctgctgcagg ccggcgacgt
     3721 gcaggtctgg cagccctggg actcgcccgg cgccgtgcgg cgctttctgg cgcactggcg
     3781 gcccgacgtc ggcgtgctga tggaaaccga gatctggccc aacctgatcc gcgaggccgc
     3841 ggcgcgcggc gtgccgctgg cgctggccaa cgcgcgcctg tccgaccgtt cgctgcgcgg
     3901 cgcccggcgc gccggcgcgc tgctgcgccc ggcctaccgc gcgctgtcgg cggtgtgggc
     3961 gcagaccgag gccgacgcgg cacgcctgcg cgccctcggc gccggcccgg tcaccgtgct
     4021 gggcaacctg aagttcgacg cccagccggc gcccgaactg ctggcgcgcg gccgcgcctg
     4081 gcgtgccggc agcgaccggc cggtggtcat gctggccagc tcgcgcgacg gcgaggaggc
     4141 tgagtttctc aagcaaatca aggccctgga gctttctggg caagcgccgg aagctattga
     4201 aaacgtagca accgaggcgg cggcgccggc cgtgcaatgg ctcatcgtgc cgcgccatcc
     4261 gcagcgattc gacgacgtgg ccgccgccgt cgctgccgcc ggcctgagcc tgagccggcg
     4321 cagcgcctgg cacgcgctgc ccgaggcggc cgaggtctgg ctgggcgaca gcctgggcga
     4381 aatggccgcg tactacgccc tggccgacgt ggccctgctg ggcggcagct ttgccccgct
     4441 gggcgggcag aacctgatcg aagcggccgc ctgcggctgc ccggtgctga tggggccgca
     4501 caccttcaac tttgcccagg ccgccgaact ggcccaggcc gcgggtgcgg cgcggcgcgt
     4561 ggccgacctg gcccaggcgg tgcacgccgc gcgcgccctg ctggccgatg cgccggcccg
     4621 cgcggccatg cgcgcggcgg cccaggactt tgcccaggcc caccgcggtg cggcagcgcg
     4681 gtcggccgag gcgctgcagg cgctgctgac gggcgccgaa ccggccgcgt gacgccggcc
     4741 accgccgcgc gcccgggcgt tggcgccgat cagcctagaa acggcagttg gacgcccccg
     4801 cccgtgccgt gatcggcgtg ccagg
//
LOCUS       DAOQTZ010000158         4778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_19806_length_4778_cov_14.407792, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000158 DAOQTZ010000000
VERSION     DAOQTZ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19806_length_4778_cov_14.407792"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PKN51_12890"
     CDS             <1..1147
                     /locus_tag="PKN51_12890"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNT86039.1"
                     /translation="IDYIGSWGPMILGHGHPAVLEAVQKAAAEGFSFGAPTEREIELA
                     EEILSLRPGMEMLRLVSSGTEAGMTAIRLARGATGRSAIIKFEGCYHGHADALLVKAG
                     SGLATFGNPTSAGVPPEVVQHTLVLEYNNLAQLEQAFAEHGPQIAGLIMEPIAGNMNF
                     VRASVDFTRRCRELCTQHGALLIYDEVMTGFRVALGGAQSVYAAAIPGFEPDLTVMGK
                     VIGGGMPLAAFGGKRAVMEQLAPLGPVYQAGTLSGNPVATACGLATLREIRKPGFFEA
                     LTARTQRLVDGLVAAARAAGVPFSADSEGGMFGFFLLPELPRNYGEVMKSDGARFNRL
                     FHGLLERGVYIAPALYEAGFVSSAHSDADIDATIDAARAVLAQLGQH"
     gene            1207..2085
                     /locus_tag="PKN51_12895"
     CDS             1207..2085
                     /locus_tag="PKN51_12895"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNT86040.1"
                     /translation="MRALLTGTLAVALLTAYTLLLFALLAPLILLKLLTRPLPPIAPL
                     DAAVVAIADTWNRLNAAGMQWASGTHWQLSLPEGLSRQAWYLVICNHQSWVDILVLQR
                     SLQGRAPFLKFFIKRELIWVPLVGLVWWALDFPFLRRRGGASGAQDLATARAACARFR
                     ALPTSVMSFVEGTRFTPAKQQAQQSPFRHLLRPKAGGVMSALEVLGDRLDAVLDVTIV
                     YPGQVPRFIDVLCGRVPHVRVHVERLPVPLAADGGPLDTAALQAWLGDRWQAKDARFA
                     QLASGHPLSGGSAAPR"
     gene            complement(2057..3685)
                     /gene="purH"
                     /locus_tag="PKN51_12900"
     CDS             complement(2057..3685)
                     /gene="purH"
                     /locus_tag="PKN51_12900"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNT86041.1"
                     /translation="MTTALISVSDKTGIVDFAHALHVLGVRLLSTGGTARLLRDAGLP
                     VTEVADHTGFPEMLDGRVKTLHPRIHGGLLARRDLLEHMAALKTHGIATIDLLVVNLY
                     PFEATVARPDCTLADAIENIDIGGPAMVRSAAKNWQGVAVLTDASQYAGVLAELKAGG
                     ISQKTRFQLAVAAFNRISDYDGAISDYLSAIDFEAGGADRVPVRSLFPAQSNGRFVKL
                     QDLRYGENAHQQAALYRDLHPAPGAIVSAQQLQGKELSYNNIADADAAWECVKQWATG
                     ASATPGQAAACVIVKHANPCGVAVGESALAAYQKAFKTDPTSAFGGIIAFNVPLDGAA
                     AEAVSQQFVEVLMAPGFTPEARAVFQAKPNVRLLQIDLSGVQPGGATPWARGQNAIDV
                     KRVGSGLLMQTADHHELKAADLKVVTKKQLTPAQIEDLLFAWKVAQYVKSNAIVFCAG
                     GMTLGVGAGQMSRLDSARIASIKAEAAGLSLQGSAVASDAFFPFRDGLDVVIDHGATC
                     VIQPGGSMRDQEVIDAADERGVAMVFTGVRHFRH"
     gene            complement(3752..4000)
                     /locus_tag="PKN51_12905"
     CDS             complement(3752..4000)
                     /locus_tag="PKN51_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HNT86042.1"
                     /translation="MSKKHIDECLRENLEAYFRDLRGTEPGGLYDMMLRAMEKPLLDV
                     VMQHAGHNQSRAAEWLGMNRNTLRKKLIEHGLMAGEKS"
     gene            complement(4004..>4778)
                     /locus_tag="PKN51_12910"
     CDS             complement(4004..>4778)
                     /locus_tag="PKN51_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNT86043.1"
                     /translation="GAQIIDINMGCPAKKVCNQWAGSALMQDEALALQIIEAVVAACA
                     PHGVPVTLKMRTGWCAAHKNAPALARAAESAGVQMVTVHGRTREQGYRGEAEYDTVAA
                     IKAALTIPVVANGDIDSPAKARAVLAATGADALMIGRAAQGRPWIFREIAHFLATGRE
                     LAPPLVLEVRRALLAHLQDHYGLYGECTGVRSARKHIGWYVRGLPGGEAFRQRMNSVE
                     SAALQWQLVADYFEELADTHERLPAQPLRPQPAPGVQAH"
BASE COUNT          666 a         1660 c         1661 g          791 t
ORIGIN      
        1 catcgactac atcggctcct ggggcccgat gatcctgggc cacggccacc cggccgtgct
       61 ggaagcggtg cagaaggccg ccgccgaggg cttttccttt ggcgcgccca ccgagcgcga
      121 gatcgagctg gccgaggaaa tcctcagcct gcgccccggc atggagatgc tgcgcctggt
      181 ctccagcggc accgaagccg gcatgaccgc catccgcctg gcgcgcggcg ccaccggacg
      241 cagcgccatc atcaaattcg agggctgcta ccacggccac gccgacgccc tgctggtcaa
      301 ggccggctcc ggcctggcca ccttcggcaa cccgaccagc gccggcgtgc cgcccgaggt
      361 ggtgcagcac accctggtgc tggaatacaa caacctggcg cagctggaac aggcctttgc
      421 cgagcacggg ccgcagatcg ccggcctgat catggagccg attgccggca acatgaactt
      481 cgtgcgcgcc agcgtcgact tcacgcgccg ctgccgcgag ctgtgcacgc agcatggcgc
      541 gctcttgatc tacgacgaag tgatgaccgg ctttcgcgtc gcactgggcg gcgcgcagag
      601 cgtgtatgcg gcggccatcc ccggcttcga gcccgacctg acggtgatgg gcaaggtgat
      661 cggcggcggc atgccgctgg cggccttcgg cggcaagcgc gcggtgatgg agcagctggc
      721 gccgctgggc ccggtgtatc aggccggcac gctgagcggc aacccggtgg ccaccgcttg
      781 cggcctggcc acgctgcgcg aaatccgcaa gcccggcttc ttcgaggcgc tcacggccag
      841 gacgcagcgc ctggtcgatg gcctggtggc cgccgcgcgt gccgccggcg tgccgttttc
      901 ggccgattcc gaaggtggca tgttcggttt cttcctgctg ccggagctgc cgcgcaacta
      961 cggcgaagtg atgaagtccg acggtgcgcg cttcaaccga ctgttccacg gcctgctgga
     1021 gcgcggcgtg tacatcgcgc cggccctgta tgaggcgggc ttcgtcagca gcgcgcacag
     1081 cgacgccgac atcgacgcca ccatcgacgc ggcgcgcgcg gtgctggcgc agctgggcca
     1141 gcactgagaa ggtgtgaacg ggccccgcat gcccaccttc tggccgcttc tgctgaccga
     1201 cctgccgtgc gtgcgctgct gaccggcacc ctcgccgtgg cgctgctgac ggcgtacacg
     1261 ctgctgctgt ttgcgctgct ggcgccgctg attctgctga agctgctgac gcgcccgctg
     1321 ccaccgatcg cgccgctgga cgccgccgtg gtggcgatcg ccgacacctg gaaccgcctc
     1381 aatgccgccg gcatgcagtg ggccagtggc acgcactggc aactcagcct gccggaaggg
     1441 ttgagccgcc aggcctggta cctggtgatc tgcaaccatc agagctgggt cgacattctg
     1501 gtgctgcaac gcagcctgca ggggcgcgcg ccgtttctca agttcttcat caagcgcgag
     1561 ctgatctggg tgccgctggt gggcctggtg tggtgggcgc tggactttcc cttcctgcgc
     1621 cggcgcggcg gtgccagcgg ggcgcaggac ctggccacgg cgcgcgccgc ctgcgcccgc
     1681 ttccgcgcgc tgccgacgtc ggtgatgagc ttcgtcgagg gcacgcgctt cacgccggcc
     1741 aagcagcagg cccagcagtc gcccttccgg cacctgctgc gccccaaggc cggcggggtg
     1801 atgagcgcgc tggaggtgct gggcgaccgg ctcgacgccg tgctggacgt gaccatcgtc
     1861 tatcccggcc aggtgccgcg cttcatcgac gtgctgtgcg gccgcgtgcc gcacgtgcgc
     1921 gtgcacgtcg agcggctgcc ggtgccgctg gccgcagatg gcgggccgct ggatacggcc
     1981 gcgctgcagg cctggctggg tgatcgctgg caggccaagg acgcgcgctt tgcgcagctc
     2041 gcgtccggcc acccgctcag tggcggaagt gccgcacccc ggtgaacacc atggccacgc
     2101 cgcgctcgtc ggcagcgtcg atcacttcct gatcgcgcat cgagccgccc ggctggatga
     2161 cgcaagtggc gccgtggtcg atcaccacgt ccagcccgtc gcggaacgga aagaaggcgt
     2221 cgctggccac cgccgagccc tgcagcgaca ggcccgccgc ttcggccttg atgctggcga
     2281 tgcgcgccga atccagccga ctcatctgcc cggcgcccac gcccagcgtc atgccaccgg
     2341 cgcagaacac gatggcgttg ctcttgacgt attgcgccac cttccaggca aacagcaggt
     2401 cttcgatctg cgccggcgtg agctgcttct tcgtcaccac cttcaggtcg gcagccttga
     2461 gctcgtggtg gtcggcggtc tgcatcagca ggcccgagcc gacgcgcttg acgtcgatgg
     2521 cgttctggcc gcgcgcccag ggcgtggcgc cacccggctg cacgccgctc agatcgatct
     2581 gcagcaggcg cacgttgggc ttggcctgaa acaccgcccg cgcctcgggg gtgaagccgg
     2641 gcgccatcag cacctcgacg aactgctggc tcaccgcctc ggcggccgcg ccgtcgagcg
     2701 gcacgttgaa ggcgatgatg ccgccgaaag cgctggtggg atcggtcttg aaagccttct
     2761 gatacgccgc cagtgcgctc tcgcccacgg ccacgccgca ggggttggcg tgcttgacga
     2821 tgacgcaggc cgccgcctga cctggggtcg ctgacgctcc cgtggcccac tgcttgacgc
     2881 attcccaggc cgcgtcggcg tcggcgatgt tgttgtagga cagctccttg ccctgcagct
     2941 gctgcgcgct gacgatggcg ccgggcgccg ggtgcaggtc gcggtacagg gcggcctgct
     3001 ggtgcgcgtt ctcgccgtag cgcaggtcct ggagcttcac gaagcggccg ttgctctgtg
     3061 caggaaaaag gcttctgacg ggcacccggt cagcgccacc agcttcgaaa tcaatagcag
     3121 acaggtagtc gctgatggcg ccgtcgtagt cgctgatgcg gttgaaagcg gccaccgcca
     3181 gctggaacct ggtcttctgg ctgatgccgc cggccttcag ctcggccagc acgcctgcgt
     3241 attgcgaggc gtcggtcagc accgccacgc cctgccagtt cttggccgcg ctgcgcacca
     3301 tggccgggcc gccgatgtcg atgttttcga tggcgtcggc cagcgtgcag tcgggccggg
     3361 ccaccgtggc ctcgaacggg tagaggttga ccaccagcag gtcgatggtg gcgatgccgt
     3421 gggtcttgag cgccgccatg tgctccagca ggtcacgccg cgccagcagg ccgccgtgga
     3481 tcctggggtg cagggtcttg acgcggccat cgagcatttc gggaaagccg gtgtgatcgg
     3541 ccacctcggt gacgggcagg cccgcgtccc gcagcaggcg ggcggtgccg ccggtggaca
     3601 gcaggcgcac gcccagcacg tgcagcgcat gcgcgaaatc aacaatgccg gtcttgtcgg
     3661 agacggagat cagggcggtg gtcatggacg cactttcttc aagtcaaaaa tggctccagc
     3721 gcttgtgctg cttgcgcaag acgctatcaa ttcaggattt ctcgccagcc atcaggccgt
     3781 gctcgatcag cttcttgcgc agggtgttgc ggttcatgcc cagccattcg gcggcgcggc
     3841 tctggttgtg gccggcgtgc tgcatgacga cgtcgagcag cggcttttcc atcgcgcgca
     3901 gcatcatgtc gtacagcccc cccggctcgg tgccgcgcag gtcgcggaag taggcctcca
     3961 ggttctcgcg caggcactcg tcgatgtgtt tcttgctcat ggttcagtgg gcctgcaccc
     4021 cgggtgcagg ctggggccgc aggggctgag cgggcaggcg ctcgtgcgtg tcggccagtt
     4081 cttcaaaata gtcggccacc aactgccatt gcagggcggc actctcgacg ctgttcatgc
     4141 gctggcgaaa cgcttcgcca ccgggcaggc cgcgcacata ccagccgatg tgcttgcgcg
     4201 ccgagcgcac gccggtgcat tcgccgtaca ggccgtagtg gtcctgcagg tgcgccagca
     4261 gcgcgcgccg cacctccagc accagcggtg gcgccagctc gcgcccggtg gccagaaagt
     4321 gcgcgatctc gcgaaagatc cagggccggc cctgggcggc gcggccgatc atcagcgcgt
     4381 ccgccccggt ggcggccagc acggcgcgcg ccttggcggg cgagtcgatg tcgccattgg
     4441 ccaccaccgg gatcgtcagc gcggccttga tggcggcgac ggtgtcgtac tcggcctcgc
     4501 cccgatagcc ctgctcgcgc gtgcggccgt gcaccgtcac catctgcacg ccggcgcttt
     4561 cggcggcgcg cgccagcgcc ggggcgttct tgtgggcggc gcaccagccg gtgcgcatct
     4621 tgagcgtcac cggcacgccg tgcggcgcgc aggcggccac cacggcctcg atgatctgca
     4681 gcgccagcgc ctcgtcctgc atcagcgcgc tgccggccca ttggttgcac accttcttgg
     4741 ccgggcagcc catgttgatg tcgatgatct gggcgccg
//
LOCUS       DAOQTZ010000159         4678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_20415_length_4678_cov_15.086957, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000159 DAOQTZ010000000
VERSION     DAOQTZ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20415_length_4678_cov_15.086957"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /gene="actP"
                     /locus_tag="PKN51_12915"
     CDS             complement(<1..244)
                     /gene="actP"
                     /locus_tag="PKN51_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667773.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNT86044.1"
                     /translation="MSTAKRIGLALATLTLAGSALAAGGDVGQAAKQSTNWIAIIMFT
                     VFVAATLWITKWAAGRTKSAADFYTAGGGITGFQNGL"
     gene            complement(241..549)
                     /locus_tag="PKN51_12920"
     CDS             complement(241..549)
                     /locus_tag="PKN51_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNT86045.1"
                     /translation="MSDPMVERVQRNPKYQQLKQARSRFGWLLTILMLVVYYGYIGLI
                     AFDKEFLARPLGSGVTTLGVPIGMAVIVFTIAIVGIYVRRANREYDALTRDILEDATQ
                     "
     gene            1043..2710
                     /locus_tag="PKN51_12925"
     CDS             1043..2710
                     /locus_tag="PKN51_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT86046.1"
                     /translation="MATATPAKAIRPMTPEERKVIFASSLGTVFEWYDFYLYGSLAAI
                     IAKQFFAGLDPTSAFIFALLAFAAGFIIRPFGALVFGRLGDMIGRKYTFLVTILIMGI
                     STFIVGILPNYASIGVAAPVILICLRLLQGLALGGEYGGAATYVAEHAPHGRRGAYTS
                     WIQTTATLGLFLSLLVILGTRTIMGEPAFNDWGWRIPFLVSILLLAVSVWIRLSLSES
                     PAFQKMKAEGKTSKAPLSESFGQWKNLKIVLLALFGLTAGQAVVWYTGQFYALFFLTQ
                     QLKVDAVTANLMIAAALVIGTPFFVVFGSLSDKIGRKPIIMAGCLLAILTYFPVFKAL
                     TEAANPDLAAAQAKNKVVVVADPAECSFQFNPTGTVKFTSSCDIAKQVLAAGSVSYEN
                     EIAPAGTPAVIKIGSHVIESYTSKGIPADEAKAKDAAFKKAVADTLKADGYPTKADPA
                     KMNKGMMVAILVYLVILVTMVYGPIAAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLP
                     TTSFAIVAQTGNMYNGLWYPIIVAAMTLVIGTLFIRETKDVDIYAED"
     gene            2850..2999
                     /locus_tag="PKN51_12930"
     CDS             2850..2999
                     /locus_tag="PKN51_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86047.1"
                     /translation="MWKIALGFVLFAALSLYVIMQAGDKVDMSGEKHGAEAVHVPEAP
                     ASAAK"
     gene            3135..>4678
                     /locus_tag="PKN51_12935"
     CDS             3135..>4678
                     /locus_tag="PKN51_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNT86048.1"
                     /translation="MTDGLIRIRGARQHNLKNLDLDLRTGELTVVTGVSGSGKSSLVF
                     DTLYAEGQRRYVETFSPYARQFLDRMDKPAVDQVEGVPPAIAIDQTNPVRSSRSTVGT
                     MTELNDHLKLLFARAGQLFDRATALPVRHDNPETIYAELQRRAARFTAGPPPGEAAPS
                     GGSEARAAGSVGAMNDPRLVITFAVELPASTSADEVQQWLSTAGFTRVQAERVEGDKK
                     VLDVVADRFRVGSAEKARVIEAIELALLRGGGRMSVYALPEQGEPELWRFSTGLHCPE
                     SDVRYGDPIPSMFSFNSAVGACPACRGFGRVIGVDWGLVIPDEKLTLRAGAIKPIQTP
                     AWKECQDDLMRHAEAAGIPRDTPWNKLTPEQRHWVIEGTPTYKEGNWNRQWYGVRRFF
                     AYLETKAYKMHIRVLLSKYRSYTACPSCGGARLKTEALLWRVGSKQDADAVIAPSQRF
                     MPQGVAWSRAQLEQLPGLCLHDLMLLPIERLARFFDRLQTPSPAGGGLGWGPAAPAST
                     DAGPSP"
BASE COUNT          768 a         1599 c         1505 g          806 t
ORIGIN      
        1 ccaggccgtt ctggaagccg gtgatgccgc caccggcggt gtagaagtcg gccgccgact
       61 tggtgcgccc ggccgcccat ttggtgatcc acagcgtggc cgccacgaac accgtgaaca
      121 tgatgatggc gatccagttg gtggactgct tggcggcctg gccgacgtcg ccgccagcgg
      181 ccagcgcgct gccagccagc gtcagggtgg ccagcgcaag cccgatgcgt tttgcggtgc
      241 tcattgggtg gcgtcctcca ggatgtcgcg cgtcagggcg tcgtactcgc ggttggcgcg
      301 gcgcacatag atgccgacga tggcgatcgt gaacacgatc accgccatgc cgatcggcac
      361 gccaagggtg gtgacgccgc tgcccagcgg acgggccagg aactccttgt cgaacgcgat
      421 caggccgatg tagccgtaat acacgaccag catcaggatc gtgagcagcc agccgaagcg
      481 gctgcgcgcc tgcttgagct gctggtactt ggggttgcgc tggacgcgct cgaccatcgg
      541 gtcgctcatg tctcggtctc cttccagggt gtgcgccggc cggatcggcg ggcgcggcgc
      601 gattctggac agaggcactg accaaccact tacgtggcgg gaaacgtcac atgcggttgc
      661 aaactcaagc tggccgtcga ccgcttgcca tcgtctgacg ggcctcattc atgccgggtt
      721 cgcggcggcg ctgaaatgca accgccgggt gtgcgcgcag cgcgaccgcg aggcgggtgc
      781 agcgagatgg cgagattttt ttgtcggggt tttcaccagc ccggcagcgg ctgaaatggc
      841 cttctgcggg ggtccgattt cgcatcgaaa aacgtggcct cagggttgtc acggggtttg
      901 tcagtctggc ttcagtttcg cgcccaccgc ctgtcggtca tcgtcagaaa acggtcaggc
      961 accgccccga catttcgccg caggacacag atcgttgcgt gtcccacagc tcgttcgcac
     1021 ccccatctca ggagacagac acatggcgac tgcaaccccg gccaaggcca tccggccgat
     1081 gacgcccgag gaaaggaagg tgatcttcgc ctcctccctg ggcaccgttt tcgagtggta
     1141 cgacttctac ctctacggtt cgctggcggc catcatcgcc aagcagttct ttgccggcct
     1201 cgaccccacc tcggccttca ttttcgccct gctggcgttt gccgccggct tcatcatccg
     1261 ccccttcggc gcgctggtgt tcggccgcct gggcgacatg attggccgca agtacacctt
     1321 cctggtgacc atcctgatca tgggcatttc cacgttcatc gtgggtatcc tgcccaacta
     1381 cgccagcatc ggcgtggccg ctccggtcat cctgatctgc ctgcgcctgc tgcagggcct
     1441 ggcgctgggc ggtgagtacg gcggcgccgc cacctacgtg gccgagcacg caccccacgg
     1501 ccgccgtggc gcctacacct cctggatcca gaccaccgcc acgctgggcc tgttcctgtc
     1561 gctgctggtg attctcggca cgcgcaccat catgggtgag ccggccttca acgactgggg
     1621 ctggcgcatt ccgttcctgg tgtccatcct gctgctggcg gtgtcggtgt ggatccgcct
     1681 gtcgctgtcc gaatcgcccg ccttccagaa gatgaaggcc gagggtaaga cctccaaggc
     1741 gccgctgtcc gagtccttcg gccagtggaa gaacctcaag atcgtgctgc tcgccctgtt
     1801 cggcctgacc gccggccagg ccgtggtgtg gtacacgggc cagttctacg ccctgttttt
     1861 cctcacgcag cagctcaagg tcgacgccgt caccgccaac ctgatgatcg ccgccgcgct
     1921 ggtgatcggc acgccgttct tcgtcgtgtt cggctccctg tccgacaaga ttggtcgcaa
     1981 gcccatcatc atggcgggct gcctgctggc catcctgacc tacttcccgg tgttcaaggc
     2041 gctgaccgaa gccgccaacc ctgacctggc cgctgcccag gccaagaaca aggtggtggt
     2101 ggtggccgac ccggccgagt gctcgttcca gttcaacccc acgggcacgg tcaagttcac
     2161 cagctcctgc gatatcgcca agcaggtgct ggccgccggt tcggtgagct acgagaacga
     2221 aatcgcgcct gccggcacgc ccgccgtcat caagatcggc agccacgtga tcgagagcta
     2281 caccagcaag ggcatccccg ccgacgaggc caaggccaag gacgctgcct tcaagaaggc
     2341 cgtggccgac accctgaagg cggacggcta ccccaccaag gccgaccccg ccaagatgaa
     2401 caagggcatg atggtcgcca tcctggtgta cctggtgatt ctggtcacca tggtgtatgg
     2461 ccccatcgcc gccatgctgg tcgagctgtt cccgacccgc atccgctaca cctccatgag
     2521 cctgccctac cacatcggca acggctggtt cggcggtttg ctgcccacca cctcgttcgc
     2581 catcgtggcg cagaccggca acatgtacaa cggcctgtgg tatcccatca tcgtggccgc
     2641 catgacgctg gtgattggca cgctgttcat ccgcgaaacc aaggacgtgg acatctacgc
     2701 cgaggattga gcctgccggc gcggtccggt cgtgctgacc ggggcgcgca tggcctgagc
     2761 cttaccccgc tcaccccagt gcctggatca gccgggcgac tggggtgagt tggttttcag
     2821 ccccccgatt cgtcattcag gagttcaaca tgtggaaaat cgccctgggt tttgtgttgt
     2881 ttgctgccct ttcgctctac gtcatcatgc aagccggtga caaggtcgac atgagcggcg
     2941 aaaagcacgg cgccgaagcc gtgcacgtgc ccgaagcgcc agcttcggcc gccaagtgac
     3001 gccgggagcc tgaatcccca acggccggcg gcccgatctg tggccgcgca cgggccggac
     3061 cacaagcccc gccggccggc agtgccaacc cgcggcccct agaatcttgc ggcccttccc
     3121 ctgacggacc cgcaatgacc gatggcctga tccgcatccg cggcgcgcgg cagcacaacc
     3181 tgaagaacct tgacctggac ctgcgcaccg gggaactgac ggtggtcacc ggcgtgtccg
     3241 gttcgggcaa gtccagcctg gtgttcgaca ccctgtacgc cgagggtcag cgccgttacg
     3301 tcgagacctt cagcccctat gcgcgccagt ttctggaccg catggacaag cccgcggtcg
     3361 atcaggtgga gggcgtgccg ccggcgatcg ccatcgacca gaccaacccg gtgcgcagct
     3421 cgcgctccac ggtggggacg atgacggagc tgaacgatca cctgaagctc ctgttcgccc
     3481 gcgccggcca gctgttcgac cgcgccaccg cgctgcccgt gcggcacgac aacccggaaa
     3541 ccatttacgc cgagctgcag cggcgcgccg cgcgcttcac cgccgggccg cctccaggtg
     3601 aagcagcccc ctcggggggc agcgaagccc gcgcagcggg gagcgtgggg gccatgaacg
     3661 acccgcgtct ggtcatcacc tttgcggtgg aactgccggc cagcaccagc gccgacgagg
     3721 tgcagcaatg gctgtcgacc gccggcttca cccgcgtgca ggccgagcgc gtggaagggg
     3781 acaagaaggt ccttgatgtg gtggcggatc gattcagggt cggcagcgcc gagaaggcgc
     3841 gcgtcatcga ggccatcgag ctggcgctgc tgcgtggcgg cgggcgcatg agcgtgtatg
     3901 ccctgcccga acagggcgag cccgaactgt ggaggttttc caccggcctg cactgccccg
     3961 aaagcgatgt gcgctacggc gatccgatcc cgtcgatgtt cagcttcaac tcggcggtgg
     4021 gcgcctgccc ggcctgccgc ggctttggcc gcgtgatcgg ggtggactgg ggactggtga
     4081 ttccggacga aaagctcacg ctgcgcgccg gtgccatcaa gccgatccag accccggcct
     4141 ggaaggagtg ccaggacgac ctgatgcgtc acgccgaggc cgccggcatc ccgcgcgaca
     4201 cgccctggaa caagctcacg cccgagcaac ggcactgggt gatcgagggc acgcccacgt
     4261 acaaggaagg caactggaac aggcagtggt acggcgtgcg gcgcttcttc gcctatctgg
     4321 agaccaaggc ctacaagatg cacatccgcg tgctcttgtc caagtaccgc agctacaccg
     4381 cctgccccag ctgcggcggc gcgcgcctga agaccgaggc gctgctgtgg cgggtgggca
     4441 gcaagcagga cgcggacgcc gtgatcgcac cgtcgcagcg cttcatgccg cagggcgtgg
     4501 cgtggagccg ggcgcaactg gaacagctcc ccggcctgtg cctgcacgat ttgatgctgc
     4561 tgccgatcga gcggctggcg cgctttttcg accgactgca aaccccttcc cccgctgggg
     4621 gagggctggg gtgggggccc gccgcacccg catcaaccga cgccggcccc tcacccca
//
LOCUS       DAOQTZ010000160         4671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_20469_length_4671_cov_13.392331, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000160 DAOQTZ010000000
VERSION     DAOQTZ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20469_length_4671_cov_13.392331"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="PKN51_12940"
     tRNA            complement(1..76)
                     /locus_tag="PKN51_12940"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Ile,seq:cat)
     gene            complement(90..1106)
                     /gene="hrcA"
                     /locus_tag="PKN51_12945"
     CDS             complement(90..1106)
                     /gene="hrcA"
                     /locus_tag="PKN51_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNT86049.1"
                     /translation="MLDDRARLLLKTLIERYIADGQPVGSRTLSRASGLELSPATIRN
                     VMSDLEELGLIASPHTSAGRVPTARGYRLFVDTMLTAQRAELEAPELPAAQPQKVIAQ
                     AAQLLSSMSQFVGVVMAPRRSSVFRHIEFLRLSERRLLLIIVAPDGDVQNRVLFTDTD
                     HTASELQQASNYLNAHFSGLDIEQVRLRLRHEVDSLRGEIAKLMHAAVQLGSEAMTQA
                     QDEVVISGERNLLAVSDFSGDLGQLRRAFDLFEQKAQLIRLLDMSSQAEGVRIFIGGE
                     SQMVPVEDLSVVSAPYEVDGQVVGTLGVIGPTRMPYDRMIQIVDITSRLVSNALSQGR
                     PGST"
     gene            1134..2030
                     /locus_tag="PKN51_12950"
     CDS             1134..2030
                     /locus_tag="PKN51_12950"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNT86050.1"
                     /translation="MSLHFHRVALVGKYQDAASGSAPESTRQVIADIAGFLVEQGCEV
                     ALAERTAVSTGLRQYPALDVPQIGTDCDLAVVVGGDGTMLGIGRQLARHGTPLIGINQ
                     GRLGFITDIPLGDYRRVLPTMLAGGYEEDPRSLMHGQVMRDGQCVFEALAMNDVVVNR
                     GAVASMVELRVEVNGRFVANHRADGIIVATPTGSTAYALSAGGPLLHPMVPAWALVPI
                     APHTLSNRPIVLADSVEIAIEIVAGRDASASFDMQSITSLHHGDRIVVRRSDHRMRLL
                     HPRGWSYFDTLRGKLHWNTGGV"
     gene            2035..3735
                     /gene="recN"
                     /locus_tag="PKN51_12955"
     CDS             2035..3735
                     /gene="recN"
                     /locus_tag="PKN51_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNT86051.1"
                     /translation="MALRHLSLRDFVIVAALDLALADGFTALTGETGAGKSILIDALQ
                     LVLGGRGDALWVREGQPRCDIAAEFELPDAARPWLAEHGFDTDADALLLRRTIDAAGK
                     SRAWINGSPATLAQLRTLGEWLVDIHGQHAWQSLTRPAAVRALLDAYAGIDTRPLTTA
                     WQAWRQADTALQAARSAQEQLAQERERLLWQIGELDKLAPGEGEWDELNQRHARLSHA
                     QALIDAAQAALAALEGDDGDGGALPALGHAHDALAARRDIEPEFATLAQQLDDAIAQA
                     RDAAHSLHGYLRRANLDPEQLLALDQRLGQWLGLARRFRRPPQELHTVLAQWRTELAQ
                     LDAAADLAQLQARADAAQQTYRQSADAVSRQRRQTAPQLAAAVTEAMQLLGMAGGRFE
                     VQLQALAEPAPHGLENVAFLVAAHAGSAPRAIERVASGGELSRLALAIAVTTSQLGTA
                     GTLVFDEVDAGVGGAVAATVGRLLQRLGRDRQVLCVTHLPQVAACADAHLQVSKQTGD
                     GLPTSQVAPLVGEARTREIARMLGGEQLSDTTLAHAREMLALPAQPPGAAARPRKVRA
                     "
     gene            3732..4643
                     /gene="rapZ"
                     /locus_tag="PKN51_12960"
     CDS             3732..4643
                     /gene="rapZ"
                     /locus_tag="PKN51_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNT86052.1"
                     /translation="MTPFAPAPSAAAAPPPTGTELVVITGMSGSGKSIALRALEDAGY
                     YCVDNLPPELLRPFIALEDQRRARRLAIAMDVRSAESLPQLPPLLDELAARGVHLRQI
                     FLEANDHTLVRRFSETRRRHPLSLADPGDVQQALLQAIARERAQMADLRERSLVIDTS
                     VLLPSQLLATVKELIAAPLAHLTLVFESFAFKRGITLDADYVFDVRMLPNPYYLPELR
                     PLTGRDQPVIDYLARQPEVQRMQAQIAQFLRHWLPALARDHRSYVTVAIGCTGGQHRS
                     VYLVEQLAAELGSSWTTLRRHRELDGL"
BASE COUNT          685 a         1721 c         1616 g          649 t
ORIGIN      
        1 tggtaggccg tgcaggactt gaacctgcga ccaagggatt atgagtcccc tgctctaacc
       61 actgagctaa cggcccgcca gcggggattc taggtgctgc ccggtcgccc ctggctgagc
      121 gcgttgctca ccaggcgcga ggtgatgtcg acgatctgga tcatgcggtc gtaaggcatg
      181 cgcgtggggc cgatcacgcc cagcgtgccg accacctggc cgtccacttc ataaggggcg
      241 ctgaccaccg acaggtcttc caccggcacc atctggcttt caccgccgat gaagatgcgc
      301 acgccctcgg cctggctcga catgtcgagc agacggatca gctgcgcctt ctgctcgaac
      361 aggtcgaacg cgcgccgcaa ctggccgagg tcgcctgaaa agtcgctcac cgccagcagg
      421 ttgcgctcgc ccgagatgac cacctcgtcc tgcgcctgtg tcatggcctc gctgcccagt
      481 tgcacggcgg cgtgcatcag cttggcgatt tcgccgcgca ggctgtcgac ctcatgccgc
      541 agccgcaggc gcacctgttc gatgtccagg ccggagaaat gggcgttcag gtagttggag
      601 gcctgttgca gctccgacgc cgtgtggtcc gtgtcggtga acagcacgcg gttctgcacg
      661 tcgccgtcgg gggcgacgat gatcagcagc aggcgccgct ccgacaggcg caggaattcg
      721 atgtggcgga acaccgacga acgccgcggc gccatcacca cgccgacgaa ctgcgacatg
      781 ctggacagca gctgcgccgc ctgcgcgatc accttctgcg gctgggcggc gggcagttcg
      841 ggggcctcca gctcggcgcg ctgggcggtc agcatggtgt cgacgaacag ccggtagccg
      901 cgcgccgtcg gcacccggcc ggccgaggtg tgcgggctgg cgatcaggcc cagctcctcc
      961 aggtcggaca tgacgttgcg gatggtggcg ggcgacagct ccagccccga cgcgcgcgac
     1021 agcgtgcgcg aacccaccgg ctgaccatcg gcgatgtagc gctcgatcag cgtcttcagc
     1081 agcaggcgtg cgcggtcgtc caacatgcaa aatcattatg atgtaatttg ctcatgagcc
     1141 ttcacttcca ccgtgtcgcg ctggtcggca aataccagga tgcggcttcc ggcagcgcgc
     1201 cggaatcgac ccgccaggtg attgccgaca tcgccggttt tctggtggaa cagggctgcg
     1261 aggtggcgct ggccgagcgc acggcggtgt ccaccggcct gcggcagtac ccggcgctcg
     1321 atgtcccgca gatcggcacc gactgcgatc tggcggtggt ggtgggcggc gacggcacca
     1381 tgctcggcat cgggcgccag ctggcgcgcc acggcacgcc gctgatcggc atcaaccagg
     1441 ggcgcctggg cttcatcacc gacattccgc tgggcgacta ccgccgcgtg ctgcccacca
     1501 tgctggcggg cggctacgaa gaagacccgc gcagcctgat gcacggccag gtgatgcgcg
     1561 acggtcaatg cgtgttcgag gcgctggcca tgaacgacgt ggtggtcaac cgcggcgcgg
     1621 tggccagcat ggtcgagctg cgggtggagg tcaatgggcg cttcgtcgcc aaccaccggg
     1681 ccgacggcat catcgtggcc acgcccaccg gctccaccgc ctacgcgctg tcggccggcg
     1741 gcccgctgct gcaccccatg gtgccggcct gggcgctggt gccgatcgca ccgcacacgc
     1801 tgtccaaccg gccgatcgtg ctggccgatt cggtcgagat cgccatcgag atcgtggccg
     1861 ggcgcgacgc cagcgccagc ttcgacatgc aatcgatcac ctcgctgcac cacggcgacc
     1921 gcatcgtggt gcggcgctcc gaccaccgca tgcgcctgct gcacccgcgc ggctggagct
     1981 atttcgacac cctgcgcggc aagctgcact ggaacaccgg aggcgtctga ccccatggca
     2041 ctgcgccacc tcagtctgcg cgacttcgtc atcgttgcgg cgctcgatct ggcgctggcc
     2101 gatggcttca ccgccctgac cggcgagacc ggcgccggca agtcgatcct gatcgacgcc
     2161 ctgcaactgg tgctgggcgg gcgcggcgac gccctgtggg tgcgcgaggg ccagccgcgc
     2221 tgcgacatcg ccgccgagtt cgaactgccc gacgcggccc gcccctggct ggccgagcac
     2281 ggcttcgaca ccgacgccga cgccctgctg ctgcgccgca ccatcgacgc cgccggcaag
     2341 agccgcgcct ggatcaacgg ctcgcccgcc accctggcgc agctgcgcac gctgggcgag
     2401 tggctggtcg acatccatgg ccagcacgcc tggcagagcc tgacgcgccc ggccgccgtg
     2461 cgcgccctgc tcgacgccta cgccggcatc gacacccggc cgctgaccac cgcctggcag
     2521 gcctggcgcc aggccgacac ggcgctgcag gccgcgcgca gcgcgcagga gcagctggcg
     2581 caggaacgcg aacgcctgct gtggcagatc ggcgagctgg acaaactggc gcccggcgag
     2641 ggcgagtggg acgagctgaa ccagcgccac gcccggctgt cgcacgccca ggcgctgatc
     2701 gacgccgccc aggccgcgct ggccgcgctg gaaggcgacg acggcgacgg cggtgccctg
     2761 ccggcgctgg ggcacgcgca cgacgcgctg gccgcgcggc gcgacatcga acccgagttc
     2821 gccaccctgg cccaacagct cgacgacgcc atcgcccagg cgcgcgacgc cgcccacagc
     2881 ctgcacggct acctgcggcg cgccaacctc gaccccgagc agctgctggc gctggaccag
     2941 cgcctgggcc agtggctggg cctggcgcgg cgcttccggc gcccgccgca ggagctgcac
     3001 acagtgctgg cgcaatggcg caccgagctg gcgcagctcg acgccgccgc cgatctggcg
     3061 cagctgcagg cgcgcgccga cgccgcgcag caaacctacc ggcagagcgc cgacgccgtg
     3121 tcgcgccagc gccgccagac cgcgccgcag ctggccgccg ccgtcaccga ggcgatgcag
     3181 ctgctgggca tggctggcgg tcgcttcgag gtccagctgc aggcgctggc cgagcccgcg
     3241 ccgcacggtc tggaaaatgt ggcatttctg gttgcggcgc atgccggatc tgcgccacgc
     3301 gctatcgaac gtgtagcatc cgggggtgaa ctgtcgcgcc tggccctggc cattgccgtc
     3361 accaccagcc agttgggcac ggccggcacg ctggtgttcg acgaggtcga cgccggcgtc
     3421 ggcggcgccg tggccgccac cgtagggcgg ctgctgcagc gcctggggcg cgaccggcag
     3481 gtgctgtgcg tcacccacct gccgcaggtg gccgcctgtg ccgacgcgca cctgcaggtc
     3541 agcaagcaga ccggcgacgg cctgcccacc agccaggtgg cgcccctggt cggcgaggcg
     3601 cgcacgcgcg aaatcgcccg catgctgggc ggcgagcagc tgtcggacac cacgctggcg
     3661 catgcgcgcg aaatgctggc gctgccggcc cagccgccgg gcgccgcggc gcggccacga
     3721 aaggtgcgcg catgaccccc ttcgcgccgg caccctcggc ggccgccgcc ccgccgccca
     3781 cgggcaccga gctggtggtc atcaccggca tgtccggctc gggcaagtcg atcgcgctgc
     3841 gggcgctgga agacgccggc tactactgcg tcgacaacct gccgcccgag ctgctgcgcc
     3901 ccttcatcgc cctggaggac cagcggcggg cgcgccggct ggccatcgcc atggacgtgc
     3961 gcagcgccga gtcgctgccg caactgccgc cgctgctcga cgagctggcc gcgcgcggcg
     4021 tgcacctgcg ccagatcttc ctggaagcca acgatcacac cctggtgcgc cggttttccg
     4081 aaacccggcg ccggcacccg ctgtcgctgg ccgacccggg cgacgtgcag caggcgctgc
     4141 tgcaggccat cgcgcgcgaa cgcgcccaga tggccgacct gcgcgagcgc tcgctggtga
     4201 tcgacaccag cgtgctgctg ccctcgcagc tgctggccac cgtcaaggaa ctgatcgccg
     4261 ccccgctggc gcacctgacg ctggtgttcg aatcgttcgc cttcaagcgc ggcatcacgc
     4321 tggacgccga ctacgtgttc gacgtgcgca tgctgcccaa cccgtactac ctgcccgagc
     4381 tgcggccact caccgggcgc gaccagccgg tgatcgacta cctggcgcgg cagcccgagg
     4441 tgcagcgcat gcaggcccag atcgcacagt tcctgcgcca ctggctgccg gcgctggcgc
     4501 gcgaccaccg cagctacgtc accgtggcca tcggctgcac cggcggacaa caccgctcgg
     4561 tctacctggt ggagcagttg gcggccgaac tggggtcgtc ctggaccacg ctgcgccgcc
     4621 accgcgaact ggacgggctg tagccgccgc cgccggccgc aggcatggtg g
//
LOCUS       DAOQTZ010000161         4659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_20538_length_4659_cov_9.954605, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000161 DAOQTZ010000000
VERSION     DAOQTZ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20538_length_4659_cov_9.954605"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKN51_12965"
     CDS             complement(<1..593)
                     /locus_tag="PKN51_12965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT86053.1"
                     /translation="MRLSTPSALDHTLASWAERLRERLDLPLLVRWQGGSLRLGQFEQ
                     ALVTVDVRDAAGATALLAPSLDNLGRAYVEGHIDVDGRVQDVIEVAHRLAEASARQDG
                     RAGWVQRAALRMASAMGHSRAQDRAAIRHHYDVSNDFYAEWLDAAMVYSCAYFETGQE
                     SLAEAQQRKIDHILTKLRLRPGQTLLDIGCGWGALVLR"
     gene            902..3052
                     /locus_tag="PKN51_12970"
     CDS             902..3052
                     /locus_tag="PKN51_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNT86054.1"
                     /translation="MGDKTEVRTDQVQARLLAHAPQGVPLGLAPEAAARQPVWLGLQI
                     SHAPDWHTYWKNAGDSGMPTELHWALPPGVLAGDIAWPLPRKIPIGQLANYGYDGTVL
                     LPVPLIITPDYKPSPLADSLEIGLKATWLVCRQECIPQDGEFSLKLPLRSSTALHGAA
                     FEAALASQPAELPGQHAITLADGGRRLALRVTGLPAAVRGQVLELFPETPNIVVTAAR
                     LAAPGEAAGERSWSQRWDGETWTADVPVSPDRAEAPARMPLVLVAGERGWRVTAPVDG
                     AWPALAPVAAMSPALEAALAANAANAGAAPPAGATPAPAGTLALALLGALVGGLVLNL
                     MPCVFPVLAIKVLGFTQHAEDRRAHRLGGLAYTLGVVLSFLALGALMLALRAAGQQLG
                     WGFQLQSPAVVAALTVLFTVIGLNLAGVFEFGQFVPSRAATLEARHPTVNSFLTGMLA
                     VAVASPCTAPFMGASLGLAVGLPALQALAVFGAIGFGMALPYLAASWWPAVARLLPKP
                     GMWMDTFKKFMAFPMFGTAVWLLWVLGQQTGIDGAGALLALLVALALVLWALGLRGRS
                     RLLLASVALVVGGFLAVAIGPNVIKAAIPSALAAAEPGARWQPWSPERVQTALGDGRP
                     VFVDFTAAWCVTCQYNKKTTLANDEVLQAMDGAKVQTFRADWTRRDAAITAELTRLGR
                     SGVPVYLLQAPGKPPIVLSEILSVADVKDALSRL"
     gene            3087..3521
                     /locus_tag="PKN51_12975"
     CDS             3087..3521
                     /locus_tag="PKN51_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNT86055.1"
                     /translation="MSVRKQRQHARRRHLVRRGLIYSLSLVGAILLLGGVGFWGLDPE
                     VHSLQDGLWLAFTTAATVGYGDVVPSTPLSRAFSVIVVLLGLAVLSLVTASLAAIFVE
                     EKVEEEERQIERDLRHEIRGLRHQVEALRAEVRHAQGEAKQP"
     gene            complement(3555..4409)
                     /locus_tag="PKN51_12980"
     CDS             complement(3555..4409)
                     /locus_tag="PKN51_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNT86056.1"
                     /translation="MSITTVGIVGAGTMGNGIAQACAVSGIDVVMVDIAQAAVDRGLA
                     TVGKSLDRLRQREKISEADKAAALSRIKGSTSYDELKGAHLVIEAATENHELKNQILK
                     QLDELLAPEVIIATNTSSISITQLAGATQRADRFVGMHFFNPVPMMALVEIIRGLQTS
                     DATHDAVKDLAVRLGKTPITVKNAPGFVVNRILVPMINEAFFVLAEGLATPEDIDAGM
                     KLGCNHPIGPLALADMVGLDVCLAVMEVYLEQFGDSKYRPCPLLREYVAAGRLGRKTG
                     VGVYSYAK"
     gene            4551..>4659
                     /locus_tag="PKN51_12985"
     CDS             4551..>4659
                     /locus_tag="PKN51_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006411520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNT86057.1"
                     /translation="MGTERRRRTMDYVIVGAGSAGCVLAARLSEDPSVQV"
BASE COUNT          604 a         1603 c         1700 g          752 t
ORIGIN      
        1 cgcagcacca gcgcgcccca gccgcagccg atgtccagca gcgtctggcc gggccgcagg
       61 cgcagcttgg tcaggatgtg gtcgatcttg cgctgctgcg cctcggccag gctttcctgg
      121 ccggtctcga aataggcgca ggaatacacc atggccgcat ccagccattc ggcgtagaag
      181 tcgttggaga cgtcgtagtg gtgccggatc gcggcgcggt cctgggcgcg gctgtggccc
      241 atcgcgctgg ccatgcgcag cgccgcgcgc tgcacccagc cggcgcggcc gtcctgccgc
      301 gcgctggcct cggccaggcg gtgggcgacc tcgatgacgt cctgcacgcg gccgtcgacg
      361 tcgatgtggc cctccacgta ggcgcggccc aggttgtcga gcgagggcgc cagcagggcg
      421 gtggcgcccg cggcgtcgcg cacgtccacg gtgaccagcg cttgctcgaa ttgccccagc
      481 cgcaggctgc cgccctgcca gcgcaccagc aggggcaggt cgagccgttc gcgcaggcgt
      541 tcggcccagg aggcgagggt gtggtccagg gcggacggtg tcgacagacg catggcttgt
      601 cagctccttg cagcggcgct gccggcaccc gatggagacc ggccagcgat ccgcgttgtc
      661 cgagacaggg gcgcaagtta acacgattgc cagcggccgt tcaagccggg agcggcagga
      721 tcgggccccc tgggctacag tcgcgagcgt cggcgcggaa cctcggcccg cgcggcggct
      781 ctgacttccg ttgcccgttt ccacccacca ccccatgtcc cgactgcgcc gtctcgcccc
      841 ctacctgttt gctatttttg cgatagcttt tggcgcaggt gtgacgggcg ccagggccca
      901 gatgggtgac aagaccgaag tccgcaccga ccaagtgcag gcacgcctgc tggcacacgc
      961 gccgcagggc gtgccgctgg ggctggcgcc cgaggccgcc gcccgccagc cggtctggct
     1021 gggcctgcag atcagccacg cgcccgactg gcacacctac tggaagaacg cgggcgattc
     1081 gggcatgccc accgagctgc actgggcgct gccgcccggc gtgctggcgg gcgacatcgc
     1141 ctggccgctg cccaggaaga ttcccatcgg ccagctggcc aactatggct acgacggcac
     1201 ggttctgctg ccggtgccgc tgatcatcac gcccgactac aagccgtcgc cgctggccga
     1261 cagcctggag atcggcctga aggcgacctg gctggtgtgc cgccaggaat gcattccgca
     1321 ggacggcgaa ttcagcctca agctgccgct gcgcagctcc accgcgctgc acggcgcggc
     1381 gttcgaggcg gcgctggcca gccagccggc cgagctgccg ggtcagcatg ccatcaccct
     1441 ggccgacggc ggtcggcgcc tggcgctgcg cgtgacgggc ctaccggcgg ccgtgcgcgg
     1501 gcaggtgctg gagttgtttc ccgagacgcc caacatcgtc gtcaccgccg cccggctggc
     1561 cgcgccgggc gaggcggcgg gcgagcgcag ctggagccag cgctgggacg gcgagacatg
     1621 gacggccgac gtcccggttt cgcccgaccg cgccgaggcc cccgcgcgga tgccgctggt
     1681 gctggtggcg ggggagcgcg gctggcgcgt gacggcgccg gtggacggcg cctggccggc
     1741 cctggcgccg gtggccgcca tgtcgcccgc gctggaggcg gcgctggcgg ccaacgccgc
     1801 caacgccggc gccgcgcccc cggcgggcgc cacgccggca ccggccggca ccctggcgct
     1861 ggccctgctg ggcgcgctgg tcggcggcct ggtgctcaac ctgatgccct gcgtgttccc
     1921 ggtgctggcc atcaaggtgc tgggcttcac ccagcacgcc gaggaccggc gcgcgcaccg
     1981 gctgggcggg ctggcctaca cgctgggcgt ggtgctgtcg ttcctggcgc tgggggcgct
     2041 gatgctggcg ctgcgtgcgg ccgggcagca gctcggctgg ggctttcagc tgcagtcgcc
     2101 ggcggtggtg gcggcgctga cggtgttgtt cacggtgatc ggcctgaacc tggcgggtgt
     2161 gttcgagttc ggccagttcg tgcccagccg cgccgccacg ctggaagccc ggcaccccac
     2221 ggtcaattcc ttcctcaccg gcatgctggc ggtggccgtg gcctcgccct gcacggcgcc
     2281 gttcatgggc gcctcgctgg gcctggcggt gggtctgccg gcgctgcagg cgctggcggt
     2341 gttcggcgcc atcggcttcg gcatggcgct gccctatctg gcggccagct ggtggccggc
     2401 ggtggcgcgc ctgctgccca agcccggcat gtggatggac accttcaaga agttcatggc
     2461 ctttcccatg ttcggcaccg cggtctggct gctgtgggtg ctgggccagc agaccggcat
     2521 cgacggcgcc ggcgcgctgc tggcgctgct ggtggcgctc gcgctggtgc tgtgggcgct
     2581 gggcttgcgc ggccgctcgc gcctgctgct ggcttccgtc gcgctggtcg tgggcggctt
     2641 tctggcggtg gccatcggcc ccaacgtgat caaggcggcg attccgtcgg cgctggccgc
     2701 cgccgagccg ggcgcgcgct ggcagccctg gtcgcccgag cgcgtgcaga cggcgctggg
     2761 cgatggccgg ccggtgttcg tcgatttcac cgccgcctgg tgcgtgacct gccagtacaa
     2821 caagaagacc acgctggcca acgatgaggt gctgcaggcc atggatgggg ccaaggtgca
     2881 gaccttccgc gccgactgga cccggcgcga cgccgccatc acggccgaac tgacccggct
     2941 tggccgcagc ggcgtgccgg tctacctgtt gcaggcgccc ggcaagccgc ccatcgtgct
     3001 gagcgagatc ctgagcgtgg ccgatgtgaa ggacgcgctc agccgcctgt aagaatcggc
     3061 gcgctcgacc acgccacaat ggcgccatga gcgtgcgcaa gcagcgacaa catgcgcgcc
     3121 gccgccatct ggtgcggcgc gggctgatct attcgctgag cctggtgggc gcgatcctgc
     3181 tgctgggtgg cgttggcttc tgggggctcg accccgaagt gcacagtctt caggacgggc
     3241 tgtggctggc cttcaccacg gcggcgaccg tgggctatgg cgacgtggtg ccgtcaacgc
     3301 ccttgtcgcg cgctttttcc gtcatcgtcg tgctgctggg tctggccgtg ctgtcgctgg
     3361 tcacggcgtc gctggcggcc atcttcgtcg aggaaaaagt cgaggaagag gagcggcaga
     3421 tcgagcgcga cctgcggcac gagattcgcg gtctgcgcca tcaggtcgag gcactgcgcg
     3481 cggaggtgcg gcacgcgcag ggcgaggcga aacagccgta gggtcaagcc agcgccgcgc
     3541 ccctgtccgc gcccttactt ggcgtagctg tacacaccca cgccggtctt gcggcccagg
     3601 cggccggcgg ccacgtattc gcgcagcagc gggcaggggc ggtacttgct gtcgccgaac
     3661 tgctccaggt acacctccat caccgccagg cacacatcca ggcccaccat gtcggccagc
     3721 gccagcgggc cgatcgggtg gttgcagccc agcttcatgc cggcgtcgat gtcttccggc
     3781 gtggccaggc cttcggccag cacgaagaag gcctcgttga tcatcggcac caggatgcgg
     3841 ttgacgacga agccgggcgc gttcttcacc gtgatcggcg tcttgccgag ccgcacggcg
     3901 aggtccttca cggcgtcgtg cgtggcgtcc gaggtctgca gaccgcggat gatctccacc
     3961 agggccatca tcggcaccgg gttgaagaag tgcatgccga cgaaccggtc ggcgcgctgg
     4021 gtcgcgccgg ccagctgcgt gatggagatc gacgaggtgt tggtggcaat gatcacctcg
     4081 ggcgccagca gttcgtcaag ttgcttgagg atctggttct tcagctcgtg gttctcggtc
     4141 gcggcctcga tcaccagatg cgcgcccttc agctcgtcgt agctggtcga gcccttgatg
     4201 cggcttaagg cggcggcctt gtcggcttcg ctgatcttct ctctttgccg caggcggtcc
     4261 aggctcttgc cgacggtggc cagccccctg tccacggccg cctgcgcgat gtcgaccatg
     4321 acgacgtcga tgcccgacac ggcgcaggcc tgcgcaatgc cattgcccat ggtgccggcg
     4381 ccgacgatgc caacggtggt gatgctcatg tgtgtgctcc tgttgagtgg gatgaaagtg
     4441 aggcgcgccc gcgctgccgg ccgcgacacc acgatggtag cggcagcgca tgtcggcccg
     4501 ccgcgctttt ggaagaaact gcctaaagtc cggcgccgcg ccgctccaca atcggtaccg
     4561 aaaggagacg tcggaccatg gactacgtga tcgtcggcgc cggctcggcc ggctgcgtgc
     4621 tggccgcgcg cctgagtgaa gatcccagcg tgcaggtga
//
LOCUS       DAOQTZ010000162         4640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_20644_length_4640_cov_14.008724, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000162 DAOQTZ010000000
VERSION     DAOQTZ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20644_length_4640_cov_14.008724"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..563
                     /locus_tag="PKN51_12990"
     CDS             201..563
                     /locus_tag="PKN51_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNT86058.1"
                     /translation="MRRLTQAPNVAIAQLWADLLCEAGYPATVERRFLSSVAGELPPD
                     QCLPEIWLRHDEHLTPARALLAELARVPQRRWRCAACGERVEGGFEQCWACGAWMPDA
                     PDCAPMDSAFGCLEYRSG"
     gene            complement(583..954)
                     /locus_tag="PKN51_12995"
     CDS             complement(583..954)
                     /locus_tag="PKN51_12995"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HNT86059.1"
                     /translation="MKAWIIAAACCAAATAWAGEDCAAARTEFETMACLHRVYQTAEQ
                     GLSARYQELVSLLDADGRKRLRVSQLAWLKSRNASCSQRKGTSTLVDPRCMAEVTTAR
                     AQSLQERIGECRAGGCLNERL"
     gene            1221..2024
                     /locus_tag="PKN51_13000"
     CDS             1221..2024
                     /locus_tag="PKN51_13000"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HNT86060.1"
                     /translation="MNSGKVRALALRAAFIAPLCLLVGATAQADINVPAGAWASLGGG
                     SMDLGCTDLIVSGNYDLGGGTLTGVRHVTVNSGGSLSFGAGGSVALAGNWANSGTITA
                     TGGTVSIGDDAGCPAPIATSVISGNTTFHNLGITSSAGKLIQFAAGSVQTVEGALTLT
                     GTGVPLRIESTTPGSVSADLRLVPAGTQNLSNLAVRGMSASGQWLAPGQTNQGSGPVS
                     RWFGDPNAAVAPIPTTSQWTLLIMALALGATAVRQRRAKLENSQEGNRS"
     gene            2021..>2262
                     /locus_tag="PKN51_13005"
     CDS             2021..>2262
                     /locus_tag="PKN51_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86061.1"
                     /translation="MTPRSPLRRLAIAAGVIFAATTAPAFAADVTITPPAGGGVVING
                     PVSMPGVPGSAVQSDKVVCAANGGALGPCPANYGATG"
     assembly_gap    2263..2362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2363..2892
                     /locus_tag="PKN51_13010"
     CDS             <2363..2892
                     /locus_tag="PKN51_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86062.1"
                     /translation="GATGPAGATGGTGPVGPMGPAGPIGPAGVQGPVGAQGPAGPTGP
                     TGATGAGQAVFFGHSAWDIPTLFPRYIGVGTIDDSEAKVAVPLPIGGTIGGLRVFQSG
                     TAGSGNSWTYTLFVNGSAVGAVSCSISGNAATTCSSAGTVAVSAGDRVALRVAPSSLP
                     STGRAAGWSFTITSP"
     gene            complement(2964..4055)
                     /locus_tag="PKN51_13015"
     CDS             complement(2964..4055)
                     /locus_tag="PKN51_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT86063.1"
                     /translation="MARPTICLNMIVKDEAHVIERCLASARPLIDRWCIVDTGSTDDT
                     QQRIQAALAGVPGTLHQRPWKNFGHNRTEALQLALAEGCEHLLFIDADEQFVVPPGFA
                     WPPLTADAYELTCHYAGTQYARRALVATRLPWHWEGVLHEYLDCPQPHRAQALAAPTI
                     LVQHDGARAHDPQTYLKDIAVLQAALAEQPDHPRYQFYLAQSQRDAGQLQAAHASYLR
                     RAALVGWDEERWYALLQAALLAERLQHPPADIAAAYLTAYAARPSRAEPLVQLARWHR
                     QQQQYALACVYAQAACALPLPADRLFVEAAVYQWQALDELAVSAYYVPVPAVQAAGRE
                     AMRKLLRQAQRLPAEVQARLRANARFYGL"
     gene            complement(4119..>4640)
                     /locus_tag="PKN51_13020"
     CDS             complement(4119..>4640)
                     /locus_tag="PKN51_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNT86064.1"
                     /translation="LADIDHGCYTDLQLTLARHPSETDERMMLRLAALALNAHQVQDL
                     CGGDGRIGFGAGLSSPDEPDVQLLDFTGRTRLWVEVGQPDDKPLAKACARADAVAVYA
                     YGPAAEVWWRGIAAKLARLPRLQVWRIAAADAQALAALAERSMQLQATVQEGQLTLSA
                     AAGSVTLEPQRWQ"
BASE COUNT          637 a         1522 c         1669 g          712 t
ORIGIN      
        1 gcccctcacc cctccctctc cccggagggg cgagggcgat cgggcgtgca ggttttcacc
       61 ctctcccgct gggagagggc tggggtgagg gccagcggcc gcagcggcgg catggtggtc
      121 ggcatcggcc ccttgcctca acggggcgcg ggcgaacgct ggcgccctcg cccatgcagg
      181 aaatgggtct ataagacgcc atgcgccgtc tgacccaggc cccgaacgtg gcgatcgccc
      241 agttgtgggc cgatctgctg tgcgaggccg gctatccggc cacggtggag cggcgctttc
      301 tgtccagcgt ggcgggcgag ctgccgcccg accagtgcct gcccgaaatc tggctgcgcc
      361 acgacgagca cctgacgccc gcgcgtgcgc tgctggccga gctggcgcgc gtgccgcagc
      421 ggcgctggcg ctgcgcggcc tgcggcgagc gggtggaagg cggcttcgag cagtgctggg
      481 cctgcggggc ctggatgccc gacgcgcccg actgtgcccc aatggattcg gcctttggat
      541 gcctcgaata tcgttcgggc tgagcggccc tgccccgcgc gcctacaggc gttcgttcag
      601 gcagccgccg gcgcggcatt cgccgatgcg ctcttgcagt gactgcgcgc gcgccgtcgt
      661 cacctcggcc atgcagcgcg gatcgaccag ggtgctggtg cccttgcgct gcgagcagct
      721 ggcgttgcgc gacttcagcc acgccagctg gctgacgcgc aggcgcttgc ggccgtcggc
      781 gtccagcagc gacaccagtt cctggtagcg cgcgctcagc ccctgctcgg ccgtctggta
      841 gacgcggtgc aggcaggcca tggtctcgaa ttcggtgcgc gcggccgcgc agtcctcgcc
      901 cgcccaggcg gtggcggcgg cgcagcaggc ggcggcgatg atccaggctt tcatggtcgg
      961 cgggtgcagc ggcggtggcc gaggcgtgtt gcccggcgcg ggcatcttaa tcgggcggca
     1021 tgtgacccga agtgcccgcg catgcggcca tcgtctcgcg caattgaggt aaaaagtgtg
     1081 actttatgcc gccaaattgt cgcggatcaa cgacaacgac gtgaacacgg tccgcgatcg
     1141 gccggatggt tgtaataatg tgaacgagtc gttaccaaaa catgcgctgg ctttgtgaat
     1201 gggccaggga ggcatcgtca atgaattcag gcaaagtcag ggcgctggcc ctcagggcag
     1261 ctttcatcgc accgctgtgc ttgctcgtgg gcgccacggc ccaggcggac atcaacgttc
     1321 ccgccggagc ttgggccagc ctgggtggcg gcagcatgga tctgggctgc accgacctga
     1381 tcgtcagcgg caactacgat ctgggcggcg gcaccctgac cggtgtgcgc cacgtcaccg
     1441 tgaacagcgg cggctctctc agcttcggtg ccggcggtag cgttgcgctg gcgggcaatt
     1501 gggccaacag cggcaccatc accgccaccg gcggcacggt ttccatcggt gacgatgccg
     1561 gttgcccggc gccaatcgcc accagcgtga tcagtggcaa caccacgttc cacaacctcg
     1621 gcatcaccag cagcgccggc aagctgatcc agtttgccgc cggctcggtg cagacggtgg
     1681 agggcgcgct cacgctcacg ggtacgggtg tgccgctgcg catcgagtcg accacgccgg
     1741 gatcggtctc ggccgacctc aggctggttc ccgctggcac gcagaacctg agcaacctgg
     1801 ccgtgcgcgg catgtcggcc agcggccagt ggctggcgcc cggccagacc aaccagggca
     1861 gcgggccggt atcgcgctgg ttcggcgacc ccaatgccgc cgtggcaccc attcccacca
     1921 cctcgcagtg gacgctgctg atcatggcgc tggcgctcgg cgcgacggcc gtgcgtcagc
     1981 gtcgcgccaa actcgaaaac tctcaggaag gaaaccgctc atgacacccc gctcccccct
     2041 gcgtcgcctg gccatcgctg ctggcgtcat cttcgcggcc actacggccc cagccttcgc
     2101 ggccgacgtc accatcacgc caccggctgg cggcggtgtg gtgatcaacg gcccggtcag
     2161 catgcccggg gtgcccggca gtgccgttca gtccgacaag gtggtgtgcg ccgccaacgg
     2221 gggtgcgctc ggtccctgtc cagccaacta tggcgccacg ggnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nncgggtgcc acaggccccg ccggtgccac gggtggtaca
     2401 ggtccggtcg gtcccatggg tccggcgggt cccatcggtc cggcaggcgt gcaaggtccg
     2461 gttggtgcac agggaccggc tggtcccacg ggtccgacgg gtgctaccgg cgccggccag
     2521 gcggttttct tcgggcactc ggcatgggac attcccacgc tttttccgcg gtacatcggt
     2581 gtcggcacca tcgatgacag cgaggccaag gttgcggtgc cattgccgat tggcggcacc
     2641 attggcggcc tgcgcgtgtt tcagagtggc acggccggca gtggcaacag ctggacctac
     2701 accctcttcg tcaatggctc ggcggtgggc gcagtcagtt gcagtatcag cggcaacgcg
     2761 gccacgacct gcagcagcgc gggcaccgtg gcggtgtcgg ctggcgaccg ggtggcgttg
     2821 cgggttgcgc ccagcagtct gcccagcacc ggacgggcag ctggctggtc attcacgatc
     2881 acgtcgccgt aagccaaagc gggaaaccca cagcaatagg gggccatgcg cccccttttt
     2941 gctgtgcatg gggaaacctt gctctacagc ccgtaaaaac gcgcattggc gcgcagccgt
     3001 gcctgtacct cggccggcag gcgctgcgcc tgccgcagca gcttgcgcat ggcctcacgt
     3061 cccgcggcct gcactgccgg caccggcacg taataggcgc tgacggccag ctcgtccagc
     3121 gcctgccact ggtaaacagc ggcttcgacg aacaggcggt cggccggcag cggcagcgcg
     3181 caggcggcct gggcgtacac gcaggccagg gcgtattgct gctgctggcg gtgccagcgc
     3241 gccagttgca ccagcggctc ggcgcggctg gggcgggcgg cataggcggt cagataggcg
     3301 gcggcaatgt cggccggcgg gtgctgcagg cgctcggcca gcagggcggc ctgcagcagg
     3361 gcgtaccagc gctcctcgtc ccagccgaca agggcggcgc ggcgcaggta gctggcgtgc
     3421 gcggcctgca gctggccggc gtcgcgctgg ctttgcgcca gatagaactg gtagcgcggg
     3481 tgatcgggct gctcggccag cgcggcctgc agcacggcga tgtccttcag ataggtctgg
     3541 gggtcgtgcg cgcgcgcgcc gtcgtgctgc accaggatgg tgggcgcggc cagcgcctgg
     3601 gcgcggtgcg gctgggggca gtccaggtat tcgtgcagca cgccctccca gtgccagggc
     3661 aggcgggtgg cgaccagggc gcggcgcgcg tactgggtgc cggcgtagtg gcaggtgagt
     3721 tcatacgcat cggccgtgag gggcggccag gcaaagccgg gcggcacgac aaactgctcg
     3781 tcggcgtcga tgaacagcag gtgctcgcag ccttcggcca gggccagttg cagcgcctcg
     3841 gtgcggttgt ggccaaagtt cttccagggg cgctggtgca gggtgccggg cacgccggcc
     3901 agcgcggcct ggatgcgctg ctgtgtgtcg tcagtggagc cggtgtcgac gatgcaccag
     3961 cggtcgatca gcggtcgggc cgaggccagg cagcgctcga tcacgtgcgc ttcgtccttg
     4021 acgatcatgt tcaggcagat cgtgggtcgg gccatggcgc tcaggtgggc aagggtttga
     4081 cgatcggggg gtggctgcag gggtggatgg ccgggcggct attgccagcg ctgcggctcc
     4141 agcgtcacgc tgccggcggc ggcagacagg gtgagctggc cctcctgcac cgtggcctgc
     4201 agctgcatgc tgcgctcggc cagcgccgcc agcgcctgcg cgtcggcggc ggcgatgcgc
     4261 cagacctgca gcctgggcag gcgcgccagc ttggccgcga tgccgcgcca ccacacctcg
     4321 gcggcggggc cgtaggcgta caccgcgacc gcgtcggcgc gcgcgcaggc cttggccagc
     4381 ggcttgtcgt cgggctggcc cacctccacc cacaagcgcg tgcggccggt gaaatcgagc
     4441 agctgcacgt ccggctcgtc cgggctggac aggccggcac caaagccgat gcggccgtcg
     4501 ccgccgcaca aatcctgcac ctggtgggca ttgagcgcca gcgcggccag ccgcagcatc
     4561 atgcgctcgt cggtttcgct ggggtggcgc gccagggtca gctgcaggtc ggtgtagcag
     4621 ccgtggtcga tgtcggccag
//
LOCUS       DAOQTZ010000163         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_20865_length_4602_cov_11.919947, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000163 DAOQTZ010000000
VERSION     DAOQTZ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20865_length_4602_cov_11.919947"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..426
                     /locus_tag="PKN51_13025"
     CDS             79..426
                     /locus_tag="PKN51_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86065.1"
                     /translation="MRPSPALPIALAACGLLIGTLVSAQTPGGAAASSLRDAPVLDSR
                     TQRVEHIQHEDAGSRVSELRVGGETRSISVQPKAGVPAYEVRPADASGTSAVQREAGP
                     GSAGPRVWKMLQF"
     gene            458..1444
                     /locus_tag="PKN51_13030"
     CDS             458..1444
                     /locus_tag="PKN51_13030"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNT86066.1"
                     /translation="MAVYTEVSEDEARALLQRLGLGELTALRGIEGGIENTNYFASTT
                     EGEWVLTLFERLTHAQLPFYLHLMKHLAQHGVPVPDPQAKPAGLPVPPGEDDLLHTLH
                     GKPTAVVNRLRGKSELSPGPAHCAAVGDMLARAHLAARDYDRRQPNLRGLAWWNETVP
                     VVLPFLDESQATLIRSELAYQNHVAAGSAYAALPRGAVHADLFRENVMFEGTAEQPVL
                     TGFFDFYFAGVDTWLFDLAVTLNDWAIDLPTGATDPARTAALLHAYQAVRPLTDAERR
                     LLPAMLRAGALRFWLSRLWDFHLPREASLLKPHDPRHFERVLRQRLAAPLTA"
     gene            1441..2232
                     /locus_tag="PKN51_13035"
     CDS             1441..2232
                     /locus_tag="PKN51_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HNT86067.1"
                     /translation="MKLRLVPTPTGVQWVRAGVRTFFRQPLALMGLFLMFLAVVSVLA
                     FVPFIGSVLALVAVPALSLGLMAAAREADEGRFPMPLTLFAALRKSPQASRAMVQLGL
                     LYAIGVMLVMGISALVDGGEFAQVYVQGGGLTPEKVMEPGFRSAMWLSTLLYLPLSLA
                     FWHAPALVHWHDVAPVKSLFFSLVAIFRNRKAYLLYGAMWLALSLGVGMVAMLLLAFV
                     LRFSGLILAATAMLPLSLVLAAIFFTSQWFTFRDSFDADSESTIP"
     gene            2229..3299
                     /locus_tag="PKN51_13040"
     CDS             2229..3299
                     /locus_tag="PKN51_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HNT86068.1"
                     /translation="MTNAWVSEAVRRIERDFQRSADTHLFPIDLPALAGVQIYLKDES
                     THPTGSLKHRLARSLFLYGLCNGWIGEGTTIIESSSGSTAVSEAYFARLLGLPFIAVM
                     PRSTSAAKVAQIAFYGGRSHFVERGDQVYDEAARLARECGGHYMDQFTYAERATDWRG
                     NNNIAESIFQQMRAEPHPVPRWIVVGAGTGGTSATIGRYLRYQGHDTQLCVVDPEHSV
                     FLDHWQDRSRTGLTSDQPSRIEGIGRPRVEPSFVPGVIDRMLRVPDAASFAALRVLEQ
                     RVGKRFGGSTGTDFCGALQLACELRARGEAGSIVTLACDGGERYLDTYYSDAWLSTQG
                     HDIGPWLDRLNAACDSGSWYGN"
     gene            complement(3331..4233)
                     /locus_tag="PKN51_13045"
     CDS             complement(3331..4233)
                     /locus_tag="PKN51_13045"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT86069.1"
                     /translation="MNITLVQMRAFLALAESLSFTRAADSLGVTQPTLSATIRHLETA
                     IGGRLFDRDTRKVALTALGLDCQRLARQLLSEVDRVEGQLRSHVLGRRGSVRIAAPAN
                     LFPNVLLPGLRAFRQANPGVRLEFADVPSDEAVHRLHAHQADLAIGVRAGGETGLRTQ
                     VVGQYPYVAILPDTHALAARRAIRWRDIQTEDVVVLQARDSLTSRIARTLGEAGVMPQ
                     AAYRVNELSTAAALVHGGFGVGLMSYWSAHQIFKPGLVIRMLSEPSLNGTIVLRTLPS
                     VELSPQVRQLQEALRRHAPTLPRL"
     gene            4415..>4602
                     /locus_tag="PKN51_13050"
     CDS             4415..>4602
                     /locus_tag="PKN51_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNT86070.1"
                     /translation="MLKTEIRGRTLVLTLDRPEVRNAWNPALARGLHEAWKRLVSDDE
                     LWSCVVTGTGAFFSAGLDL"
BASE COUNT          740 a         1606 c         1491 g          765 t
ORIGIN      
        1 ggcgcggtgg cgggtgtggg gtcgctgctc atcgcggcat tgtgcctgcg gcgcagcgct
       61 gcgccataaa atgaggctat gcgcccatcc cctgccctcc ccatcgcgct ggccgcctgc
      121 ggtctgctga tcggcaccct ggtgtcggcc cagacaccgg gcggcgccgc ggcctcgtcg
      181 ctgcgcgatg ccccggtgct cgacagccgc acgcagcgcg tggaacacat ccagcatgaa
      241 gatgccggca gccgcgtcag cgaactgcgc gtgggcggcg aaacccgcag catcagcgtg
      301 cagcccaagg ccggcgtgcc ggcctacgaa gtgcgcccgg ccgacgccag cggcaccagc
      361 gccgtgcagc gcgaagccgg ccccggcagc gccggcccgc gggtctggaa gatgcttcag
      421 ttttaatagc gcacgccgca caccccacaa gcgcgacatg gcggtttaca ccgaagtctc
      481 cgaagacgag gcccgcgccc tgctgcagcg gctgggcctg ggcgagctga ccgcgctgcg
      541 cggcatcgaa ggcggcatcg aaaacaccaa ctacttcgcc agcacgaccg agggcgaatg
      601 ggtgctgacg ctgttcgagc gcctgacgca cgcgcagctg ccgttttacc tgcacctgat
      661 gaagcacctg gcccagcacg gggtgccggt gcccgatccg caggccaagc ccgccgggct
      721 gcccgtcccg ccgggcgaag acgacctgct gcacaccctg catggcaagc ccacggcggt
      781 ggtcaaccgt ctgcgcggca agagcgagct cagccccggc ccggcgcact gcgccgccgt
      841 gggcgacatg ctggcgcgcg cccacctggc ggcacgcgac tacgaccggc gccagcccaa
      901 cctgcgcggg ctggcctggt ggaacgagac ggtgccggtg gtgctgccgt ttctggacga
      961 atcgcaagcc acgctgatcc gcagcgagct ggcctaccag aaccatgtcg ccgccggcag
     1021 cgcctacgcc gcgctgccgc gtggcgcggt gcatgccgac ctgtttcgcg aaaacgtgat
     1081 gttcgagggt acggccgaac aacccgtgct gacgggtttt ttcgacttct actttgccgg
     1141 cgtcgacacc tggctgttcg acctggcggt tacgctcaac gactgggcca tcgacctgcc
     1201 caccggcgcc accgacccgg cgcgcacggc cgcgctgctg cacgcctacc aggcggtgcg
     1261 cccactcacc gacgccgagc ggcgcctgct gcctgccatg ctgcgtgccg gcgccctgcg
     1321 cttctggctg tcgcgcctgt gggacttcca tctgccgcgc gaagccagcc tgctcaaacc
     1381 gcatgacccg cgccacttcg agcgcgtgct gcgccagcgc ctggcggcgc cactgacggc
     1441 atgaagctgc gcctggtccc caccccgacc ggcgtgcagt gggtgcgcgc gggcgtgcgc
     1501 accttctttc gccaaccgct ggcgctgatg gggctgttcc tcatgtttct ggcggtggtg
     1561 tcggtgctgg ccttcgtgcc cttcatcggc agcgtgctgg cgctggtggc cgtgccggcg
     1621 ctctcgctgg gcctgatggc cgccgcgcgc gaagccgacg agggtcgatt tcccatgccg
     1681 ctgactctgt ttgcggcctt gcgcaagagc ccgcaggcca gccgcgccat ggtgcagctg
     1741 ggcctgctgt atgccatcgg cgtgatgctg gtgatgggca tttcggccct ggtcgatggc
     1801 ggcgagtttg cgcaggtcta cgtgcagggc ggcggcctca cccccgagaa agtgatggag
     1861 ccgggctttc gcagcgcgat gtggctgtcc accctgctgt acctgccgct gtcgctggcg
     1921 ttctggcacg cgcccgcgct ggtgcactgg cacgacgtgg cgccggtgaa aagcctgttc
     1981 ttcagcctgg tggccatctt caggaaccgc aaggcctacc tgctgtacgg cgcgatgtgg
     2041 ctggcgctgt cgctgggtgt cggcatggtg gcgatgctgc tgctggcctt cgtgctccgg
     2101 ttcagcggcc tcatactggc ggccaccgcc atgctgccgc tttcgctggt gctggccgcg
     2161 atcttcttca cctcgcagtg gttcaccttc cgcgacagct tcgacgccga ctccgaaagc
     2221 actatcccat gaccaacgcc tgggtcagcg aagccgtgcg gcgcatcgag cgcgactttc
     2281 aacgttcggc cgacacccac ctgtttccga tcgatctgcc cgcactggcc ggcgtgcaga
     2341 tctacctgaa ggacgaatcc acccacccca ccggcagcct caagcaccgg ctggcgcgct
     2401 cgctgtttct gtacgggcta tgcaatggct ggattggcga gggcaccacc atcatcgaat
     2461 cgtcctcggg cagcacggcg gtgtcggagg cctacttcgc gcgcctgctc ggtctgccct
     2521 tcatcgccgt gatgccacgc agcacctcgg ccgccaaggt ggcgcagatc gccttctacg
     2581 gcgggcgcag ccacttcgtc gagcgcggcg atcaggtgta cgacgaggcc gcccggctgg
     2641 cgcgcgaatg cggcggccac tacatggacc agttcaccta tgccgagcgc gccaccgact
     2701 ggcgcggcaa caacaacatc gccgaatcga tcttccagca gatgcgcgcc gagccgcacc
     2761 cggtgccgcg ctggatcgtg gtcggcgcgg gcactggcgg cacctccgcc accatcggcc
     2821 gctacctgcg ctaccagggc cacgacaccc agttatgcgt ggtcgatccc gagcactcgg
     2881 tgttcctcga ccactggcaa gaccgctcgc gcaccggcct caccagcgat cagccgtcac
     2941 gcatcgaagg catcggccgc ccgagggtgg agccgtcctt cgtacccggt gtgatcgacc
     3001 gcatgctgcg cgtgcccgac gctgccagct ttgccgcgtt gcgcgtgctg gagcagcgcg
     3061 tgggcaagcg ctttggcggc tccaccggca ccgatttttg cggcgcgctg caactggcct
     3121 gcgagctgcg cgcacgcggc gaggcgggct ccatcgtcac cctggcctgc gatggcggcg
     3181 agcgctatct ggacacgtac tacagcgacg cctggttaag cacccagggc catgacatcg
     3241 gcccctggct ggatcgattg aatgccgcct gcgacagcgg cagctggtac ggcaactgaa
     3301 agcaatcccg gcgcgccatc ctgtaactct ctacagacgt ggcagcgtcg gcgcatggcg
     3361 ccgcagcgct tcctgcaact ggcgcacctg gggcgacagc tcgacgctgg gcaaagtccg
     3421 cagaacgatg gtgccgttca agctgggttc gctgagcatg cggatcacca gcccgggttt
     3481 gaaaatctgg tgcgccgacc agtaactcat cagtccaacg ccaaaaccgc catggaccag
     3541 cgctgcagca gtggatagct cattgacccg atacgctgcc tgcggcatca ccccggcctc
     3601 gcccagggtg cgtgcgatgc gagatgtcag ggaatcacga gcctgcagca ccaccacgtc
     3661 ctcggtctgg atatcgcgcc atcggatcgc acgtcgggca gccagcgcat gggtgtcagg
     3721 caggatcgcc acataggggt actggcccac cacctgcgta cgtaatccgg tttcaccgcc
     3781 tgcacgcacg ccgatggcca ggtcagcctg gtgtgcatgc agccggtgta cggcctcatc
     3841 gctgggcacg tcggcaaact ccaggcggac gcctggattc gcctggcgaa atgcccgcaa
     3901 tcctggcagc aaaacgttag ggaacaggtt ggccggcgcc gcaatgcgta ccgacccgcg
     3961 ccgccccagc acatgactgc gcagctggcc ttcgacacga tcaacctcgc tgagcagttg
     4021 ccgcgccagc cgttggcagt ccaatccgag cgcggtcaag gccaccttgc gcgtgtcccg
     4081 atcaaacagt ctgccgccaa tcgccgtctc cagatggcgg atggtggcgc tgagcgtggg
     4141 ttgcgtaacg cccagactgt ctgccgcccg cgtgaagctc agcgactcgg caagcgcaag
     4201 aaacgcacgc atctgaacca gggtgatatt catagatgaa gtctatagcc atatatgcac
     4261 atccgatcaa taccagccta gactttccgc atgcaggagc ccccctgagc cgccccacgt
     4321 gggccggtgg cgatcgcacg gttgatgcac gccattgctg aggcacgagg aacacgcctg
     4381 actcacgagt ttctcagtcc agaaaggaaa accaatgctg aagacggaaa tacggggtcg
     4441 cacactggtg ttgaccctgg atcgcccgga ggtacgcaac gcctggaacc ccgctctggc
     4501 gcgaggtctg cacgaggcat ggaagcgcct ggtgtcggat gacgaactgt ggagctgcgt
     4561 ggtcactggg accggcgcgt ttttctcggc cggtctggac ct
//
LOCUS       DAOQTZ010000164         4591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_20919_length_4591_cov_14.578263, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000164 DAOQTZ010000000
VERSION     DAOQTZ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20919_length_4591_cov_14.578263"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKN51_13055"
     CDS             complement(<1..683)
                     /locus_tag="PKN51_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86071.1"
                     /translation="MHRKTFLIAAACLGSCAAGALAADAGTGLTSRASRAVQAPFDIV
                     HTRISTQGQVATFRMTVSGRAGASRPARSGKLAGSKVFSYVWPTSLDPAVVGFEPKAG
                     ILAFAVTAHPDFDDTPLFDENGDGRLDNDGNVWHSHWVVLQPDAACGKDALKVVDIPA
                     GSKPRLPKTWPGLPILIDSPGWSPTLKGRTLEVRVPFDDIGAVESGAFDGVTAALRVN
                     ASVHSPLLCV"
     gene            898..1776
                     /locus_tag="PKN51_13060"
     CDS             898..1776
                     /locus_tag="PKN51_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT86072.1"
                     /translation="MQTAPPFDRLSGILERFRLRARLHHAGALCGLHHFDAGEGHGYL
                     HVLRRGQLAVSHPGAREVPQALHFEQPTLLLYPRPFTHHFHNPPDEGADFTCARLDFE
                     GGAEHPLARALPPLIALPLARVEGLGLALELLFAETDRPRCGHRLLVDRIFEVVVIQL
                     LRWLLDHPQEAGVRPGFVTGMAHPGLARALIAMHDAPGEGWTLARLAACAGMSRTAFA
                     VTFRDVVGQTPADYLSDWRVALAQTRLRAGQPVKAMAEELGYAHPSALSRAFGARVGM
                     SPRQWLAQVSAGDAPR"
     gene            complement(1788..3425)
                     /locus_tag="PKN51_13065"
     CDS             complement(1788..3425)
                     /locus_tag="PKN51_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNT86073.1"
                     /translation="MACLRIVVLVSTLALGAPSQAQSPPSTQASDPRAMGWMQGFPPP
                     ADKTIRFTDPDYFSFPKLRWTVCHFRELMPTAGVDRGPGAASALPQAPEPGIDALRFT
                     PLGGQASMSWGEAFDANHTDGLLVLHHGRVVYERYAGCLGPHTLHGAMSLTKSITGLL
                     GEVLVAEGALDENARVGTLIPQLQDSAFGDASVRQVLDMTTALQFSEDYADPNAEVWQ
                     HAQAGSTLPPPAGYTGPRSYYESLQQIRKSGEHGQVFGYKTPNADALGWLLARTTGKP
                     LNELLAERIWRRIGAEREAFYTVDAIGTPFAGGGFNVTLRDLARLGQLILQQGEWQGV
                     QLLPSAAIERIQRGGERAPFARAGYAQLPGWSYRAMWWHSGDAHGAFSARGVHGQTLW
                     IDPVADVVIARLASHPVAANAANDPTSLPAWRAVTKHLMAQDQTPLLGGQWVIEDVAG
                     AGVIDRTRAFLRFLADGRLVGHATCNGLRGHHTSGARGRLRLRLTGITRKRCAPALMH
                     QEERLLALLPRVTRYRIDDSGALWLYTAAGKTLTARR"
     gene            3892..4377
                     /locus_tag="PKN51_13070"
     CDS             3892..4377
                     /locus_tag="PKN51_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNT86074.1"
                     /translation="MPPPNAAAARPAPELNVSQWFNTDTAPTLAALRGQVVMLHAFQM
                     LCPACVRLATPQAQQVHEALGRQGLVVLGLHTVFEHHEAMRPVSLAAYIHENRLRFPI
                     GVDRADDLHDLPLTMRAYGMQGTPTLLLIDRQGRLRQHSFGHVPDLVLGAAIGTLLGE
                     P"
BASE COUNT          664 a         1563 c         1653 g          711 t
ORIGIN      
        1 acgcacagca gcgggctgtg cacgctggcg ttgacgcgca gcgccgcggt cacgccgtcg
       61 aaggcaccgg attcgacggc gccgatgtcg tcgaacggca cccgcacctc cagcgtgcgg
      121 cccttcagcg tggggctcca gcccgggctg tcgatcagga tcggcaggcc cggccaggtc
      181 ttgggcaggc gcggcttgct gccggccggg atgtccacca ccttcagcgc gtccttgccg
      241 caggcggcat cgggttgcag caccacccag tgcgaatgcc agacgttgcc gtcgttgtcc
      301 agccggccat cgccgttttc gtcgaacagc ggcgtgtcgt cgaagtcggg gtgggcggtg
      361 accgcgaacg ccagaatgcc ggccttgggc tcgaagccga ccacggccgg gtccaggctg
      421 gtcggccaga cataggaaaa caccttgctg ccggccagct tgccggatcg ggcagggcgg
      481 ctggcgccgg cccggcccga caccgtcatg cggaacgtgg ccacctgtcc ctgggtcgag
      541 atgcgggtgt ggacgatatc gaacggagcc tgcacggccc ggctggctcg gctggtcagg
      601 ccagtgccgg catcggccgc cagggcgccg gcggcgcagc tgcccaggca ggcggcggcg
      661 atcagaaagg ttttgcggtg catcacgatc ggctcctttg aacgaagggg atggattcag
      721 tatgggggca tgtgttgtac gataagcacc caatcgttca caaaacatac ctgatcgttc
      781 agactggaaa cggcagctgg gtgcgcgcac gtcatgggtg aacgtcatcg aagccatgaa
      841 cgccagcgcc catgcggctg gcacgaagat cgcaagcggt cagctgcact tggttcaatg
      901 caaacggccc cacccttcga ccgcctgtcg ggcatcctgg agcgctttcg cctgcgggcg
      961 cgcctgcacc acgccggcgc gctgtgtggt ctgcaccact tcgatgcggg cgaagggcat
     1021 ggctacctgc acgtgctgcg ccgcggccag ctggcggtca gccatcctgg cgcgcgcgag
     1081 gtgccgcagg cgctgcactt cgagcagccc acgctgctgc tgtacccgcg cccgttcacg
     1141 caccactttc acaacccgcc ggatgagggc gcggacttca cctgtgcccg gctggacttc
     1201 gaaggcgggg ccgagcaccc gctggcgcgc gcactgccgc cgctgatcgc gctgccgctg
     1261 gcgcgcgtgg aaggcctggg gctggcgctg gagctgttgt ttgccgaaac cgatcgccca
     1321 cgctgcggcc accggctgct ggtggaccgg atcttcgagg tggtggtgat ccagttgctg
     1381 cgctggctgc tcgaccaccc gcaggaggcc ggcgtgcgcc ccggctttgt caccggcatg
     1441 gcgcacccgg gtctggcgcg cgccctgatc gccatgcacg atgcgccggg cgagggctgg
     1501 acgctggcgc gcctggccgc gtgcgccggc atgtcgcgca cggcgtttgc cgtcacgttt
     1561 cgcgacgtgg tcggtcagac cccggccgac tacctgagcg actggcgcgt ggcactggcg
     1621 cagacccggc tgcgcgccgg tcagccggtc aaggccatgg ccgaagagct gggctacgcc
     1681 cacccgtcgg cgctgtcgcg cgcctttggc gccagggtgg ggatgtcgcc gcggcagtgg
     1741 ctggcgcagg tgtcggcggg tgatgcgccg cgctgatcgc ggccggttca gcgccgcgcg
     1801 gtaagcgtct tgccggcggc cgtgtacagc cacagggcgc cgctgtcgtc gatgcggtag
     1861 cgggtgacgc gcggcagcag cgccagcagc cgctcctcct ggtgcatcag cgccggcgcg
     1921 cagcgcttgc gggtgatgcc cgtcaggcgc agccgcaacc ttccccgggc gccgctggtg
     1981 tggtggccgc gcagaccgtt gcaggtggca tggccgacca ggcgcccgtc ggccagaaag
     2041 cgcaggaagg cgcgcgtgcg gtcgatcacg ccggcgccgg cgacgtcctc gatcacccat
     2101 tggccgccca gcaggggggt ctggtcctgc gccatcaggt gcttggtcac ggcgcgccag
     2161 gccggcagcg aggtcgggtc gttggcggcg ttggcggcca ccgggtgcga ggccagacgc
     2221 gcgatcacca cgtcggccac cggatcgatc cacagcgtct gaccgtgcac gccgcgcgcg
     2281 ctgaaggcgc cgtgcgcgtc gccgctgtgc caccacatgg cgcggtagct ccagcccggc
     2341 agctgcgcgt agccggcgcg ggcgaacggc gcgcgttcgc cgccgcgctg gatgcgctcg
     2401 atcgccgccg acggcagcag ctgcacgccc tgccattcgc cctgctgcag gatcagctga
     2461 cccaggcgcg ccagatcgcg cagcgtgacg ttgaagccac cgccggcaaa tggcgtgccg
     2521 atggcgtcga cggtgtagaa ggcttcgcgt tcggcgccga tgcggcgcca gatgcgctcg
     2581 gccagcagtt cgttcagcgg cttgccggtg gtgcgcgcca gcagccagcc cagggcgtcg
     2641 gcattcgggg tcttgtagcc aaaaacctgg ccgtgctcgc cgctcttgcg gatctgctgc
     2701 agcgattcgt agtagctgcg cgggccggtg tagcccgccg gcggcggcag cgtgctgccg
     2761 gcctgggcat gctgccagac ttcggcgttc gggtcggcgt agtcctcgga aaactgcagc
     2821 gccgtcgtca tgtccagcac ctggcgcacg ctggcgtcgc cgaaggcgct gtcctgcagc
     2881 tgcgggatca gggtgccgac gcgggcgttt tcgtctagcg cgccttcggc caccaacact
     2941 tcgcccagca ggccggtgat cgacttggtg agcgacatgg cgccgtgcag cgtgtgcggg
     3001 cccagacagc cggcgtagcg ctcgtagacg acgcggccgt ggtgcagcac cagcaggccg
     3061 tcggtgtggt tggcgtcaaa ggcttcgccc cagctcatcg acgcctggcc gcccagcggg
     3121 gtgaagcgca gtgcgtcgat acccggctcc ggcgcctgcg gcagcgcgct ggcggcaccg
     3181 gggccgcggt cgacaccggc ggtgggcatc agctcgcgaa agtggcacac cgtccagcgc
     3241 agcttgggga agctgaaata gtccgggtcg gtgaaacgga tggtcttgtc ggccggcggc
     3301 ggaaagccct gcatccagcc catggcgcgc gggtcgctcg cctgggtgga cggcggcgac
     3361 tgagcctggc tcggggcgcc gagggcgagt gtgctgacca gcacaacgat gcgcagacat
     3421 gccacggtca agcccacggc ccatcggtca tggaagtcgt caccagtcat tttggaacgc
     3481 gcgtcggtgg ttggagagcg ctggcaaggg ccatggtagc cgcagcgcgt gtgccatcgt
     3541 ggccagcgtg gataccctgc ggcgcgcgtc ggcggggcgt gcctggtgca gtgtttcgcg
     3601 ctgtcaggca cttatagaag ccagaaaaag gcttgcggca aggcgcggct cgcagcccat
     3661 gcggcggcct tggcaagcgg cgcaacgccg ccgcaagcct ttttccggct tcccgcaggg
     3721 cgagccgccc gggggccatc tgcgcggcgc gtggccttga catggctcca ccatgcctgc
     3781 ggccacgcgc cgcgcagctg gccccttggc gactcgctaa aagtgcctgg cagcgcgaag
     3841 cactgcacta cctgcccata atggcctgcc caccaccctg gaagctctgc catgccaccg
     3901 cccaacgccg ctgccgcccg tccggcgccc gagttgaacg tgagccagtg gttcaacacc
     3961 gacaccgcgc cgacgctggc cgcgctgcgc ggtcaggtgg tgatgctgca tgcgttccag
     4021 atgctgtgcc cggcctgcgt gcgcctggcg acgccgcagg cgcagcaggt gcatgaagcg
     4081 ttgggccgtc agggcctggt ggtgctgggt ctgcacacgg tgttcgaaca ccacgaggcc
     4141 atgcggccgg tgtcgctggc ggcctacatc cacgaaaacc gcctgcgttt tccgattggc
     4201 gtggatcgcg cggacgacct ccacgatctg ccgctcacca tgcgtgccta cggcatgcaa
     4261 ggcacgccca cgctgctgct gatcgaccgt cagggacgct tgcgccagca cagctttggc
     4321 catgtgccgg acctggtgct gggcgcggcc atcggcacct tgctgggcga accctgagcc
     4381 agcgcccggc gaaggcgcgc gcgatcgcag cgactcgcgt ccacgatcga cgtgttgcat
     4441 gctctaccat caggtgaagg tgtgcgaagc ccggtgaaga agcggtcgtc acgaagtaca
     4501 cgagccgtca actgcccgtt cccagattca atccaaaagc ggcagttgga cgcgcccgcc
     4561 ggtgccgtga tcggcgtgcc aggcaagacg c
//
LOCUS       DAOQTZ010000165         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_21099_length_4563_cov_13.250887, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000165 DAOQTZ010000000
VERSION     DAOQTZ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21099_length_4563_cov_13.250887"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKN51_13075"
     CDS             <1..826
                     /locus_tag="PKN51_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT86075.1"
                     /translation="HLNSGTTIPASKIYHECGLPMVTGAATNPDLTKPGYKQIFRIIA
                     NDSALGAGMARYAKDVLKLKSVAVIDDRTAYGQGLADIFANTAKSLGLQVVERQFTTD
                     KATDFMAILTAIKAKKPEGIFFGGMDPQAGPMLRQMDQLGMSKTPFMGGDGVCTVKLA
                     ELSAGAKSLANAACAEGGTSLQKMPGGMEWKKRYDAKFPNQYQVYSPYTYDATMLLAD
                     AMKRADSVDPKVYLAKLAESNFKGITSTIAFESNGEVKNAAITISTYPGGKKTPLQ"
     gene            complement(1127..1996)
                     /locus_tag="PKN51_13080"
     CDS             complement(1127..1996)
                     /locus_tag="PKN51_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT86076.1"
                     /translation="MSARHLWLALVVVAIWGSNFVVIKLALAHLPPLLLATLRFALAA
                     VPLVLLVRRPPVSWGNLALYGLAIGVGQFGLLFFVMTRHISPGLASLVIQSQVFFTVG
                     LAMLLSGERVRPYQVVAALIAVAGIVLIASRTDGQTTPLGLALTLAAALSWAVGNIAS
                     RAAGPVDVLAYVAWSSLLAVPPLAVLSLTLEGWPRIAAGLVQADWATWAAVLWQSVGN
                     TIFGYGVWAWLLARYPAATVAPFSLLVPVFGMGASAALLAEPLPAWKLAAAALVMGGL
                     ALNLLWPRWRFNH"
     gene            complement(2014..4005)
                     /gene="mnmC"
                     /locus_tag="PKN51_13085"
     CDS             complement(2014..4005)
                     /gene="mnmC"
                     /locus_tag="PKN51_13085"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HNT86077.1"
                     /translation="MPDPIVHWRADGTPTSTRFDDVYRSAGSDGQAGLAQARQVFLRG
                     CGLLPEGDAAAAWAGAAHWAVLETGFGLGLNFLAAWQAWRQDPQRPARLFYSAVEAWP
                     PTADDLRRSAAPYPELRPLAEQLATQWHGLRPGVHRLGFDAEQVQLTLAVGEVQAALA
                     ELGGGHDSLWLDGFSPARNPEMWQLHTLKAAARLLRRGARAATWCVAAQVRRDLQTCG
                     FEVERVPGLPPKRHALRARYAPRWPTALERTAAPAVRPGRCAVIGAGLAGASVAWSLA
                     QRGWQVTVLDRAAQPAGGASGLPAGVIAPHVSPDERPLSRLTQAGARATLARAALLLQ
                     GGIDFAASGVLERHAPGARRLPEPAQGSNAVAHDSLDADHPLTRQRAAAASVPLDPHR
                     RALWHTHAGWIRPPALVRAMLQAPGVVWRANTAVARIEPAADGWQLRGADGTLLAAAD
                     LVVVAAGFDSLALAGAPLPLHALRGQVAFGPMPGGAADAALPAFPVNGLGSLIGHLPG
                     ADGPWWITGSTFERARTLPDCTDADHASNRQRLAELLPAAAAALDAQWSDGRAQAWAA
                     VRATLPDRLPAVGAWPSQAPPDLMPKPAPAPYQQAPAAIDSEAPTARLPLQLCTGLGA
                     RGLTLAVLCGELLAAGLHGEPLPLARSLAQRLRAERFLS"
     gene            complement(4192..4464)
                     /locus_tag="PKN51_13090"
     CDS             complement(4192..4464)
                     /locus_tag="PKN51_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNT86078.1"
                     /translation="MARMVHCIKLGTEAEGLDFPPYPGELGKRIWENVSKQAWQEWLR
                     HQTMLVNENRLNMADQRARDYLKRQMENHFFGAGADHVAGYVPPGS"
BASE COUNT          710 a         1626 c         1650 g          577 t
ORIGIN      
        1 tcacctgaac tcgggcacca ccatcccggc gtccaagatc tatcacgagt gcggcctgcc
       61 catggtcacc ggcgccgcca ccaaccccga cctgaccaag ccgggctaca agcagatctt
      121 ccgcatcatc gccaacgaca gcgccctggg cgccggcatg gcgcgctatg ccaaggacgt
      181 gctcaagctg aagtcggtgg ccgtcatcga tgaccgcacc gcctacggcc agggtctggc
      241 cgacatcttc gccaacaccg ccaagtcgct gggtctgcag gtggtcgagc gccaattcac
      301 caccgacaag gcgaccgact tcatggccat cctgaccgcc atcaaggcca agaagcccga
      361 aggcatcttc tttggcggca tggacccgca ggccggcccg atgctgcgcc agatggatca
      421 gctgggcatg agcaagaccc cgttcatggg tggcgacggc gtgtgcaccg tgaagctggc
      481 cgagctgtcc gctggcgcca agtcgctggc caacgcggcc tgcgccgagg gcggcacctc
      541 gctgcagaag atgcccggcg gcatggaatg gaaaaagcgc tacgacgcca agttcccgaa
      601 ccagtatcag gtctacagcc cctacaccta cgacgccacc atgctgctgg ccgacgccat
      661 gaagcgcgcc gactcggtcg atccgaaggt ctacctggcc aagctggccg agtccaactt
      721 caagggcatc acctcgacca tcgccttcga atccaacggc gaagtcaaga acgccgccat
      781 caccatttcc acctacccgg gtggcaagaa aacgccgctg cagtgatcgg cgtgacgacc
      841 cggtcgtcct gaaaagccgc cttcgggcgg cttttttcat cctaggttca acctagaaac
      901 ggcggttgga cgcgcccgcc cgtgccgtga tcggcgtgcc aggcaagacg cgacgccgcc
      961 gcgggctcgt gcccgcaagc aggagcaacg ccgcatggcg cgccgagcgc ggtgcgggtg
     1021 ggtgcgtagc gtgctcaccc aggaggtgaa ggccatcgca gccgccagac cccgcatcga
     1081 tgcggtctgc ccgaagttga ccagcggtca actgccgctt ctaggttcaa tggttgaagc
     1141 gccagcgtgg ccacagcagg ttgagcgcca gcccgcccat caccagcgcg gcggcggcca
     1201 gcttccaggc aggcagcggc tcggccagca gcgccgccga agcgcccatg ccgaacaccg
     1261 gcaccagcag cgaaaacggc gccaccgtcg ccgccgggta gcgtgccagc agccaggccc
     1321 agacgccgta gccgaagatg gtgttgccga ccgactgcca cagcacggcg gcccaggtgg
     1381 cccagtccgc ctgcaccaat ccggcggcga tgcgtggcca gccctccagg gtcaggctga
     1441 gcacggccag cggtggcacc gccagcaggc tggaccaggc cacgtaggcc agcacgtcga
     1501 ccgggccggc ggcgcggctg gcaatgttgc cgaccgccca ggacagggcg gcggccagcg
     1561 tcagcgccag tcccagtggc gtggtctggc cgtcggtacg gctggcgatc agcacgatgc
     1621 cggcgacggc gatcagcgcc gccaccacct ggtaggggcg cacccgctcg ccgctcagca
     1681 gcatggccag cccgaccgtg aagaacacct gcgactggat caccagcgaa gccagcccgg
     1741 gtgagatgtg gcgcgtcatg acgaagaaca gcagcccgaa ctggccgacg ccgatggcca
     1801 gcccgtacag cgccaggttg ccccaggaca ccggcgggcg ccgcaccagc agcaccagcg
     1861 gcacggcggc cagggcaaag cgcagcgtgg ccagcagcag cggcggcagg tgcgccagcg
     1921 ccagcttgat gacgacgaag ttgctgcccc agatggcgac caccaccagg gccagccaca
     1981 ggtggcgcgc gctcagcgcg gtacgggaag gactcatgac aggaaccgtt cggcacgcag
     2041 gcgttgcgcc agcgagcgcg ccagcggcag cggctcgccg tgcagcccgg ccgccagcag
     2101 ctcgccgcac agcaccgcca gggtcaggcc gcgcgcaccc aggccggtgc acagctgcag
     2161 cggcaggcgc gccgtgggtg cttctgaatc aatagctgct ggcgcttgct ggtagggcgc
     2221 tggagctggt tttggcatca aatccggcgg tgcttggctc ggccaggcgc cgaccgccgg
     2281 caggcggtcg ggcagggtgg cgcgcaccgc cgcccaggct tgggcccggc cgtcggacca
     2341 ttgcgcgtcc agcgcggcgg cggccgccgg cagcagctcg gccaggcgct ggcggttgct
     2401 ggcgtggtcg gcgtcggtgc agtcgggcag cgtccgggcg cgctcgaagg tagagccggt
     2461 gatccaccag gggccgtcgg cgcccggcag gtggccgatc aggctgccca gcccgttgac
     2521 cggaaaggct ggcagcgccg cgtcggccgc gccgcccggc atcggcccga aggcgacttg
     2581 gccgcgcaag gcgtgcagcg gcagcggggc gccggccagc gccagcgaat cgaagccggc
     2641 ggccaccacc accagatcgg ccgcggccag cagggtgccg tcggcgccgc gcagctgcca
     2701 gccgtcggcg gcgggctcga tgcgcgccac cgccgtgttg gcacgccaca caacgccggg
     2761 cgcctgcagc atggcgcgca ccagcgccgg tgggcggatc cagccggcgt gtgtgtgcca
     2821 cagggcgcgc cggtgtgggt cgagcggtac gctggcggcg gcggcgcgct ggcgcgtcag
     2881 cgggtggtcc gcgtccaggc tgtcgtgggc gacggcgttg gagccttgcg ccggctcggg
     2941 caggcgccgc gcgccgggcg cgtggcgctc cagcacgccg ctggcggcga agtcgatgcc
     3001 gccttgcagc agcagcgcgg cgcgtgccag cgtggcgcgc gcaccggcct gcgtcaggcg
     3061 cgagagcggg cgctcgtcgg gcgagacatg gggcgcgatc actccggccg gcaggccgct
     3121 ggcaccgccg gccggctgcg cggcgcgatc cagcaccgtc acctgccagc cgcgctgcgc
     3181 caggctccag gccaccgagg ccccggccag gccggcgccg atgacggcgc agcggcccgg
     3241 gcgcacggcc ggcgcggcgg tgcgttccag ggcggtgggc cagcgcgggg cgtagcgggc
     3301 gcgcagggcg tggcgcttgg gtggcaggcc gggcacgcgt tcgacctcga agccgcaggt
     3361 ctgcaggtcc cggcgcacct gggcggccac gcaccaggtg gcggcgcggg cgccgcgccg
     3421 cagcagccgg gccgccgcct tgagggtgtg cagctgccac atctcggggt tgcgggcggg
     3481 actgaagccg tccagccaca ggctgtcgtg accgccgccg agttcggcca gcgctgcctg
     3541 gacctcacct accgccagcg tcagctgcac ctgctcggcg tcgaagccca gccgatgcac
     3601 gcccggccgc aggccatgcc actgcgtcgc cagctgctcg gccagcggcc gcagctcggg
     3661 atagggcgcg gcgctgcgcc gcaggtcgtc ggcggtgggc ggccaggctt cgaccgcgct
     3721 gtagaacagc cgcgcaggcc gctgcggatc ctgtcgccag gcctgccagg cggccagaaa
     3781 gttcaggccc aggccgaacc cggtttccag caccgcccag tgcgcggcac cggcccaggc
     3841 tgcggcggcg tcgccctcgg gcagcaggcc gcagccgcgc aggaacacct gccgtgcctg
     3901 cgccagccca gcctggccgt cgctgccggc gctgcggtac acatcgtcga agcgcgtgct
     3961 ggtgggggtg ccgtcggcgc gccagtggac gatcgggtca ggcatgaaat actatcaaaa
     4021 caatagctgt tggcgcttgt tgcgagcgcg ccagaggctg atttgaccat gaacttggtg
     4081 cgggcccgag ggcctgccgg ggccatgccg acgcagacgg cggcgcgtca cccacgaacc
     4141 ctgctgcagc caggcagcgc gcgttcgggc ccgatgcgga gcgcgcccgc ctcagctgcc
     4201 gggcggcacg tagccggcca cgtggtcggc gccggcgccg aagaagtggt tttccatctg
     4261 gcgcttgagg taatcgcgcg ccctctggtc ggccatgttg aggcggtttt cattgaccag
     4321 catggtctgg tggcgcagcc attcctgcca ggcctgcttg ctgacgtttt cccagatgcg
     4381 cttgcccagc tcgcccgggt agggcggaaa gtccaggccc tcggcctcgg tgccgagctt
     4441 gatgcaatga accatgcgtg ccatgggaag gtccttgtga ggttcgacgg tgttcgcgtt
     4501 ggggacgacc cgctggcgcg ggcgcggtgg cggaactgta gcaagctttg ccgcccgcac
     4561 cgc
//
LOCUS       DAOQTZ010000166         4516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_21383_length_4516_cov_12.199955, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000166 DAOQTZ010000000
VERSION     DAOQTZ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21383_length_4516_cov_12.199955"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PKN51_13095"
     CDS             complement(<1..1034)
                     /locus_tag="PKN51_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNT86079.1"
                     /translation="MTAVATTYRTCPRSGLQFERQAEMLIKAHAFVGVVWLLIGGLLA
                     IGVVLTRWPAVRWLEADTFYMVLTAHGLDMLIFWIIFFEIAILHFCSSTLLRCRIATP
                     KIAWLGFALMLIGSILNNVAVFRGGSSVMMTSYVPMMAEPSFYLGIILFAVGALIGCF
                     VFFGTLVVARREKTYEGSIPLVTFGALTAAIIAVFTLLSGAMIIVPAWLMSLGLMNVD
                     PLIYRTVWWALGHSSQQINVAAHVSVWYLVAAIALGAKPMSERVSRTAFLLYILFLQL
                     ASAHHLLADPGVGTAWKVVNTSYFMYLAVLASMLHGLTVPGAMEVAQRQKGYTKGLFE
                     WLRKAPWGNPT"
     gene            complement(1045..1584)
                     /locus_tag="PKN51_13100"
     CDS             complement(1045..1584)
                     /locus_tag="PKN51_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HNT86080.1"
                     /translation="MSAILPPSDRLWYKHPIDRVEGTWLAIALVWCLILFFMMVGWHI
                     WGKQNLSTETYRTTPDKFMAKAQAVVDKYTVRTETDEKIPVVAPPPGSDVYLLGRLWS
                     FWPILELEKGKTYRLHITAMDYNHGLSLQPANINIQLVPGFEHVVTVTPNQSGTYGLV
                     CNEYCGIGHHRMVSRLYVK"
     gene            complement(1603..1773)
                     /locus_tag="PKN51_13105"
     CDS             complement(1603..1773)
                     /locus_tag="PKN51_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86081.1"
                     /translation="MSPHDHDALNDPNAPIPWMQQLLDSPFLLLLLGVLIPMVLYNLW
                     GVVEILLLPVAK"
     gene            2122..2973
                     /locus_tag="PKN51_13110"
     CDS             2122..2973
                     /locus_tag="PKN51_13110"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNT86082.1"
                     /translation="MLSVITVSAFIRLSQSGVGCADWPVCYEQAAGHASAHPWLGLAR
                     ATHRVTASAMLLVVLGLLVATGRRTPDLRHERRLALGVLLLTLWLAGLGALMRGASAP
                     AVVLGNLLGGLATLALCGRLLVCGSDRTAPAPSLRRWAWWAMLLVFFQAAFGGLIDAR
                     HAASSCQHLSECADLARAAGWDWRVLSPWPAASAAGELAPQARGAWLHLLHRLGALLL
                     VPVLATLAWKALRQGFSIPAIGVFVLLVLELTLGSLLAATGFPLPLALAHNLVAALLL
                     TLLAGLA"
     gene            complement(3098..4390)
                     /locus_tag="PKN51_13115"
     CDS             complement(3098..4390)
                     /locus_tag="PKN51_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNT86083.1"
                     /translation="MSQQWKFETQSVHAGYTPDPTTKSVAVPIYQTVAYAFDSAQHGA
                     DLFDLKVPGNIYTRIMNPTTDVLEKRVAALEGGIGALAVASGQAAILYALQTITEAGD
                     NIVSSTALYGGTYNLFAHTLPQYGIQTRFADHRDPASFAKLIDERTKAVYVETIGNPA
                     GNITDIASVAAIAHQHGVPLIVDNTVGTPYLIRPFEHGADIVVHSLTKYMGGHGTTLG
                     GAIVDSGKFPWAQHKARFRRLNEPDVSYHGVVYTEALGAAAYIARARVVPLRNTGAAI
                     SPFNAWQILQGIETLALRMDRICDNTLKVAQYLSQHPKVAWVSYAGLPDHEDHALAQH
                     YMQGRASGLFTFGVKAAQGGREAGARFLDALQLFTRLVNIGDTRSLATHPASTTHRQL
                     SPEELAKAGVREETVRLCIGIEHIDDLIADLDQALAKV"
BASE COUNT          738 a         1473 c         1530 g          775 t
ORIGIN      
        1 gtcgggttgc cccagggcgc cttgcgcagc cactcgaaca ggcccttggt gtagcccttc
       61 tggcgctgcg ccacttccat cgcgcccggc accgtcagac cgtgcagcat cgaggccagc
      121 accgccaggt acatgaagta gctggtgttg acgaccttcc aggccgtgcc cacgcccggg
      181 tcggccagca ggtggtgggc gctggccagt tgcaggaaca ggatgtagag caggaaggcg
      241 gtgcggctga cgcgctcgct catgggtttg gcgcccaggg cgatggccgc caccagatac
      301 cagaccgaca cgtgagccgc cacgttgatc tgctgcgacg aatgccccag cgcccaccac
      361 acggtgcggt agatcagcgg gtcgacgttc atcaggccca gcgacatcag ccaggccggg
      421 acgatgatca tggcgcccga cagcagcgtg aagacggcaa tgatggcggc cgtcagggcg
      481 ccgaaggtca ccagcggaat cgagccttca taggtctttt cgcgccgcgc caccaccagg
      541 gtgccgaaga acacgaagca gccgatcagg gcgccgacgg caaacaggat gatgcccagg
      601 tagaagctgg gttcggccat catcggcaca tagctggtca tcatgacgct ggagccgcca
      661 cggaacaccg ccacgttgtt gaggatggag ccgatcagca tcagcgcaaa gccgagccag
      721 gcgatcttgg gcgtggcgat gcgacagcgc agcaaggtcg atgaacagaa atgcaggatc
      781 gcgatctcga agaagatgat ccagaagatc agcatgtcca gaccgtgtgc ggtcagcacc
      841 atgtagaagg tgtcggcctc gagccagcgc acggccggcc agcgcgtcag cacgacgccg
      901 atcgccagca ggccgccgat cagcagccag accacgccca cgaaggcgtg ggccttgatc
      961 agcatctcgg cctggcgctc gaactgcagc ccggagcgag ggcaggtacg gtaagtggtt
     1021 gccacggcgg tcatccctgt ctcctcattt cacgtacagg cggctgacca tccgatggtg
     1081 gccaatgccg cagtattcgt tgcacaccag cccataggtg ccggattggt tgggcgtcac
     1141 cgtcaccaca tgctcgaagc cgggcaccag ctggatgttg atgttggccg gctgcagcga
     1201 caggccgtgg ttgtagtcca tggccgtgat gtgcaggcgg taggtcttgc ccttttccag
     1261 ctccaagatc ggccagaacg accacaggcg ccccagcaga tagacgtcgc tgccaggcgg
     1321 cggagccacc accgggatct tttcgtcggt ctcggtgcgc accgtgtact tgtcgaccac
     1381 ggcctgtgcc ttggccatga acttgtcggg tgtggtgcga taggtttcgg tggagaggtt
     1441 ctgcttgccc cagatgtgcc agccgaccat catgaaaaac agaatcagac accagaccag
     1501 cgcaatggcc agccaggtgc cctcgacgcg gtcgatcggg tgcttgtacc agagccgatc
     1561 tgacggggga agaatggcgc tcatgtgagt gttctcctgg agttacttgg ccacgggcag
     1621 aagcaggatc tcaaccaccc cccacaggtt gtagagaacc atcgggatca gcacccccag
     1681 caacagcaac agaaaggggc tgtcgagcag ttgctgcatc cacgggattg gggcgttggg
     1741 gtcgttcagg gcgtcatggt catgcgggct catgggttct cctccggagg ggtgggcgcg
     1801 tgagcagcgg ttcctcagcg ccggatcgca ttctttaccc gaccaaacca atcggacttg
     1861 ccgcagatca acaaatcccg gtcagaccgg ccagtgcccc ctcgggcaaa ccccggcttg
     1921 accatctttc cagtaggggc gtgccaccgc taacatgcgc tccacccgct gccgcccaca
     1981 cccaggcgcg cgccacccat gcccaacacc gctcctgtgc cgacccccca gcccctgccc
     2041 gacacccagc ccgagcatct cgatccgttg cagcgccgcc gccgacgcat acgctcgctg
     2101 gcagcgctgg cctggctgct gatgctgagc gtgatcaccg tcagcgcctt catccggctg
     2161 tcgcagtcgg gcgtgggatg cgcagactgg ccggtctgct acgaacaggc ggccggacac
     2221 gccagcgccc acccatggct ggggctggcg cgcgccaccc accgcgtgac ggccagcgca
     2281 atgctgctgg tggtgctggg tctgctggtg gccacggggc gacgcacgcc ggacctgcgc
     2341 cacgagcgcc ggctggcgct gggggtgctg ctgctgaccc tgtggctggc cggcctgggc
     2401 gcgctgatgc gcggcgccag tgcgccggcg gtggtgctgg gcaacctgct gggtggcctg
     2461 gccacgctgg ccctgtgtgg gcggctgctg gtgtgtggca gcgaccgcac ggcgcccgca
     2521 ccgtccctgc ggcgctgggc ttggtgggcg atgctgctgg tgttttttca ggccgccttc
     2581 ggcggcctga tcgacgcccg gcacgccgcc agcagctgcc agcacctgtc cgaatgcgcc
     2641 gacctggcgc gcgccgccgg ttgggactgg cgtgtgctgt cgccctggcc cgccgccagc
     2701 gccgccggcg agctggcgcc gcaggcgcgt ggcgcctggc tgcacctgct gcatcggctc
     2761 ggcgccctgc tgctggtgcc cgtgctggcg acgctggcgt ggaaggccct gcgccagggc
     2821 ttcagcatcc cggccatcgg cgtgtttgtg ctgctggtgc tggaactcac gctgggcagc
     2881 ctgctggcag ccaccggttt cccgctgccg ctggcgctgg cccacaacct ggtggccgcc
     2941 ctgctgctca cgctgctggc cgggctggcc tgagcgaatt gcacaccgcc acacgcaaca
     3001 agctcaaaag cgcccagaag cgaccgtcga tcatcgccgc gtgctggatg caggctacgc
     3061 tctggcgatg tcatgaattc cgagatcttc aataggatca gaccttggcc agcgcctgat
     3121 ccaggtcggc gatcaggtcg tcgatgtgct cgatgccgat gcacaggcgc accgtctctt
     3181 ccctgacgcc ggccttggcc agctcctcgg gcgagagctg gcggtgcgtg gtgctggccg
     3241 ggtgcgtggc cagcgagcgc gtatcgccga tgttgaccag tcgggtgaac agctgcaacg
     3301 cgtccagaaa gcgcgcgccg gcctcgcgcc cgccctgcgc cgccttgacg ccgaaggtga
     3361 acaggcccga ggcgcgcccc tgcatatagt gctgggccag tgcgtggtct tcgtggtcgg
     3421 gcagcccggc gtagctgacc caggcgacct tgggatgctg gctcaggtac tgcgccacct
     3481 tcagggtgtt gtcgcagatg cggtccatgc gcagcgccag ggtttcgatg ccttgcagga
     3541 tctgccaggc gttgaagggc gaaatggcag cgccggtgtt gcgcagcggc accacgcgcg
     3601 cgcgcgcaat gtaggccgcc gcgcccagcg cctcggtgta gaccacgccg tggtagctca
     3661 cgtccggctc gttcaggcgc ctgaagcgcg ccttgtgctg ggcccaggga aacttgccgc
     3721 tgtcgacgat ggccccgccc agcgtggtgc cgtgcccacc catgtacttg gtcagcgagt
     3781 gcaccacgat gtcggcgccg tgctcgaacg gccggatcag gtagggcgtg ccgacggtgt
     3841 tgtcgacgat cagcggcacg ccgtgctggt gggcgatggc ggccacgctg gcgatgtcgg
     3901 tgatgttgcc ggccgggttg ccgatggttt ccacatacac cgccttggtg cgctcgtcga
     3961 tcagcttggc aaagctggcc gggtcgcggt gatcggcaaa gcgggtctgg atgccgtact
     4021 gcggcaaggt atgggcaaac aggttgtagg tgccgccgta cagcgcggtg ctgctgacga
     4081 tgttgtcgcc cgcctcggtg atggtctgca gcgcgtacag gatggccgcc tggccgctgg
     4141 ccaccgccag cgcgccgatg ccaccttcca gcgccgccac gcgcttttcg agcacgtcgg
     4201 tggtcgggtt catgatgcgg gtgtagatgt tgcccggcac cttcagatcg aacaggtcgg
     4261 cgccgtgctg cgcgctgtca aaggcgtagg ccacggtctg gtagatcggc accgccaccg
     4321 acttggtggt cggatccggc gtgtagccgg catgaacgga ctgggtctca aacttccatt
     4381 gctgcgacat gagggaactc cttggaacac cgaaattgct acattgaaca tagctgccgg
     4441 cgcttgctgg atgcgcgcta cagccatttt tgttcaagaa tccatggggt ctggccgagc
     4501 caccgccagt ggcccg
//
LOCUS       DAOQTZ010000167         4498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_21499_length_4498_cov_13.096106, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000167 DAOQTZ010000000
VERSION     DAOQTZ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21499_length_4498_cov_13.096106"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKN51_13120"
     CDS             <1..627
                     /locus_tag="PKN51_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86084.1"
                     /translation="PSIPPTATTCSQIQSFLAAGKGVVSEWNGATLLIQPAAGAFHAV
                     TNPCNLFAGTAAGGLNSFGIDLPLTITAPASPLVAGLSNPFAMAGGSEFVYQLTGLGP
                     EWSVAATYTGGGAVFPAVLSAVRSGGGCVALSPFDFFDARQSGGTAQSNMETLLNNMV
                     AWAARGPASCGRAPAAAVQPVPTLETPLLAALAGLLGVCGAWLRRRRH"
     gene            complement(736..1017)
                     /locus_tag="PKN51_13125"
     CDS             complement(736..1017)
                     /locus_tag="PKN51_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNT86085.1"
                     /translation="MTLTPPPTHPPGHDHRTPEAIYGFDARGVAKRFRHAAIFGALDS
                     LHPGEVMRFVNDHDPLPLLGQMQARYGAAVRVEYRQRAPEGVVIDFIRQ"
     gene            complement(1050..1373)
                     /locus_tag="PKN51_13130"
     CDS             complement(1050..1373)
                     /locus_tag="PKN51_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNT86086.1"
                     /translation="MTFVVTEACIACKYTDCVDVCPTDAFREGPNFLVIEPDDCIDCA
                     VCVPECPVDAIYADEDVPKAQLDFIALNAELARGWKTISRKKPALPDADAWAQVGRKR
                     EQLQR"
     gene            1517..2467
                     /gene="cbiB"
                     /locus_tag="PKN51_13135"
     CDS             1517..2467
                     /gene="cbiB"
                     /locus_tag="PKN51_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNT86087.1"
                     /translation="MPDAVLLACAPALALAVDRLLGEPPARAHPVVWMGRWLGWAGAR
                     LAPAAGAPAQRSDAGVFWLAALSWCAAAATIFVVSALFQHWVLTQPAWLAALALGLFL
                     KPLLAWAMLHDEVLAVEQALAESPQAGRARLARLVSRDVAALAPHQVREAAIESLAEN
                     LNDSLVAPLFWFVLLGLPGAALFRFANTADAMWGYRGERGGRDWTWAGKWAARADDAL
                     AWLPARITALLIGALGRLRGWHRLPAEARRTPSPNGGWPMAAMALALGVRLGKPGVYT
                     LHAAGRVVQPSDTLRACAIGSHGALAATLLACVALVVAVA"
     gene            2677..3726
                     /locus_tag="PKN51_13140"
     CDS             2677..3726
                     /locus_tag="PKN51_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT86088.1"
                     /translation="MQVPAPGGATGLDSPLHGGPDAAGVPLHDLSTNANACGPWPAAV
                     QALAALDARHYPDPAYTALVRQLADWHGVAPARIVLAASASEFIQRISVAVRLQAGRA
                     AGVWLPPHAYGDYARAAQAAGLARCADPAAAALLWACEPSSPLGQAEPGLAARVAALR
                     PGQTLVLDQAYEPLRLSGAPSLDAAALQRVWRLITPNKALGLTGVRAAYAIAPLHADA
                     ALLARLRALAPSWPLGAHGVALLQGWASAPAHDWLAACRDTLRGWKLRQLEWLSRLGW
                     QIEPSDANFFVAACADPASAEGRLDMQTWLCGLRRHGIKLRDCASFGLPGRVRLAVAP
                     PPVQDALLAALEARR"
     gene            3723..>4498
                     /locus_tag="PKN51_13145"
     CDS             3723..>4498
                     /locus_tag="PKN51_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86089.1"
                     /translation="MTAVCVMVLGTSSGAGKSWITTALCRHYANQGLRVAPFKAQNMS
                     NNARVVAGGPSPQPSPQRGEGASPGALMEAGPSPQPFPEGWEGAGTARLHPLPRAGEG
                     RGEGAPSPAGGGLGWGPAPAHAGGEIGSAQYFQALAARAVPDVRMNPLLLKPEADTRS
                     QVVLLGQVSHELSALPWRGRSERVWPQIAAALDALRADNDVVVIEGAGSPAEINLMAS
                     DVVNLRVAHHADARCLLVADIDRGGAFAHLYGTWALLPEA"
BASE COUNT          562 a         1525 c         1710 g          701 t
ORIGIN      
        1 cctagcatcc ctcccacggc caccacgtgc agccagattc agagcttcct ggccgctggc
       61 aagggcgtgg tctccgaatg gaacggcgcc accttgctga tccagcccgc cgcaggcgca
      121 ttccatgccg tgaccaatcc ctgcaacctg tttgccggca cggcggctgg cggacttaac
      181 tcgttcggaa tcgacctgcc cctcaccatc accgcccctg ccagcccgct ggtggccggg
      241 ttgtccaatc catttgccat ggctggcgga agcgaattcg tctatcagtt gaccggcctg
      301 gggccggaat ggtcggtggc agccacctac accggcggcg gcgctgtgtt cccggccgtg
      361 ctgtcggcgg tgcgcagcgg tggaggctgt gtggcgctgt cgccgttcga tttttttgat
      421 gccaggcaaa gcggtggtac ggcgcagagc aacatggaaa ccttgctgaa caacatggtg
      481 gcctgggcag cccgtgggcc tgccagttgc ggtcgtgcgc cggcggccgc ggtacagccg
      541 gtgcctacgc tcgagacccc gctgctggca gcgctggcag gattgctggg tgtgtgtggg
      601 gcctggttgc gccgtcggcg tcactgatca ggcgggttga gccgcccttc cgcgcggcgc
      661 cggggtcttg cccggcgccg cgttgctttt cgggtgggac agttcccttg gctgagggcg
      721 gcgcggcgcc cgaacctact gacggatgaa gtcgatcacc acgccctcgg gggcgcgctg
      781 gcggtattcg acgcgcactg cggcgccgta gcgcgcctgc atctggccca gcagcggcag
      841 cgggtcgtgg tcgttgacga agcgcatcac ctcgcccgga tgcaggctgt cgagggcgcc
      901 gaagatggcg gcgtggcgaa agcgcttggc cacgccgcgg gcgtcgaagc cgtagatggc
      961 ttcgggcgtg cggtggtcgt ggccgggtgg gtgggtcggc ggcggggtca gggtcatggt
     1021 ggcaaggctc cggggggcgg gggtggggtt cagcgttgca gttgctcgcg cttgcggccc
     1081 acctgcgccc aggcgtcggc atcgggcagg gcgggcttct tgcgcgagat ggtcttccag
     1141 ccacgcgcca gttcggcgtt gagggcgatg aagtcgagct gcgccttggg cacgtcctcg
     1201 tcggcataga tggcgtcgac cgggcactcg ggcacgcaga cggcgcagtc gatgcagtcg
     1261 tcgggctcga tgaccagaaa attcggccct tcgcggaagg cgtcggtcgg gcatacgtcc
     1321 acgcagtcgg tgtacttgca ggcgatgcag gcttcggtga cgacaaaggt catgatgtgc
     1381 cacccgtggc gaaacattca aagatgcaca aagaatacct ttttaaggga tgccctgcca
     1441 gcaacgcggg caaacgcgcc cgccgcgcgg cgccggccgg ggtggcggcg tgatcgggct
     1501 gtctgccgcc gtcgccatgc ccgacgccgt gctgctggcc tgcgccccgg cactggcgct
     1561 ggccgtcgac cgcctgctgg gcgagccgcc ggcgcgcgcg cacccggtgg tgtggatggg
     1621 gcgctggctg ggctgggccg gtgcgcggct ggcgcccgcc gcgggggcgc cggcacagcg
     1681 ttcggatgcc ggggtgtttt ggcttgcggc gctgtcctgg tgcgcggcgg cagctacgat
     1741 ttttgtagtc agcgcgctgt ttcagcactg ggtgctgacg cagccggcct ggctggcggc
     1801 gctggcgttg gggctgtttc tgaagccgct gctggcctgg gccatgctgc acgacgaggt
     1861 gctggcggtg gagcaggcgc tggccgagtc gccccaggcc gggcgcgcac ggctggcgcg
     1921 gctggtcagc cgcgacgtgg cggcgctggc gccgcaccag gtgcgcgagg ccgccatcga
     1981 aagcctggcc gaaaacctga acgattcgtt ggtggcgcct ctgttctggt tcgtgctgct
     2041 gggcctgccg ggggcggcgc tgtttcgctt tgccaacacc gccgacgcca tgtggggcta
     2101 ccggggtgaa cggggcggac gcgactggac ctgggctggc aagtgggccg cgcgcgccga
     2161 cgacgcgctg gcctggctgc cggcacgcat cacggcgctg ctgatcggcg cgctggggcg
     2221 gctgcgcggc tggcaccggc tgccggccga ggcgcgccgc acgccgtcgc ccaacggcgg
     2281 ctggccgatg gcggccatgg cgctggcgct gggcgtgcgc ctgggcaagc cgggcgtgta
     2341 cacgctgcac gccgccggcc gggtggtgca gccgtctgat accctgcgcg cctgcgccat
     2401 cggctcgcac ggcgcgctgg cggccacgct gctggcttgc gtggcgctgg tggtggcggt
     2461 tgcttgaggc tgaacagccg gaacatccct acgcagagga cgcagagatt gcgcagaggg
     2521 cgccaagaat tcagaagaag ctttcttggt ttcttctgcg tcccctgcgc attcttggcg
     2581 tcctctgcgt atggatgttg atgggcctcc aacgcccgcc caacctgcgc caacagctcc
     2641 tgatatcgta gcaatcaagc atccatgtcc tgcaccatgc aagtgcccgc cccgggtggc
     2701 gcgaccggcc tggacagtcc gctgcacggc gggccggacg cggcgggcgt gcccttgcac
     2761 gacctgtcga ccaacgccaa cgcctgcggc ccctggccgg cggccgtgca ggcgctggcg
     2821 gcgctggatg cgcgccacta ccccgacccg gcctacaccg cgctggtgcg gcaactggcc
     2881 gactggcacg gcgtggcgcc ggcgcgcatc gtgctggcgg ccagcgccag cgaattcatc
     2941 cagcgcatca gcgtggcggt gcggctgcag gcgggcaggg cggccggcgt ctggctgccg
     3001 ccacacgcct atggcgacta cgcccgcgcc gcgcaggccg ccggcctggc gcgctgtgcc
     3061 gacccggccg ccgccgcgct gctgtgggcc tgcgagccgt ccagcccgct gggccaggcc
     3121 gagccggggc tggcggcgcg ggtggcggcg ctgcggccgg gccagaccct ggtgctcgat
     3181 caggcctacg agccgctgcg cctgtcgggg gcgcccagcc tggacgcggc ggcgctgcag
     3241 cgcgtgtggc gcctgattac gcccaacaag gcgctgggcc tgaccggtgt gcgcgcggcc
     3301 tatgccatcg cgccgctgca cgccgatgcc gcgctgctgg cccgcctgcg cgccctggcg
     3361 ccgtcgtggc cgctgggcgc gcacggcgtg gcgctgctgc agggctgggc cagcgcgccg
     3421 gcacacgact ggctggccgc ctgccgcgac acgctgcgcg gctggaagct gcgccagctc
     3481 gaatggctga gccgcctggg ctggcagatc gagcccagcg atgctaattt ttttgtagct
     3541 gcttgcgcag atccggcaag cgcagaaggt cgattggata tgcaaacatg gctttgcggc
     3601 ctgcgccgcc acggcatcaa gctgcgcgac tgcgccagct ttggcctgcc cggccgggtg
     3661 cgcctggcgg tggcgccacc gccggtgcag gacgcgctgc tggccgccct ggaggcgcgg
     3721 cgatgacggc ggtctgcgtg atggtgctgg gcaccagcag cggcgccggc aagagctgga
     3781 tcaccaccgc cctgtgccgc cactacgcca accagggcct gcgcgtggcg ccgttcaagg
     3841 cgcagaacat gagcaacaac gccagggtgg tggcgggcgg cccctcaccc cagccctctc
     3901 cccagagggg cgagggagca agccccggtg ccttgatgga ggcgggcccc tcaccccaac
     3961 cctttcctga ggggtgggag ggagccggaa cggcgcggct gcacccgctc ccgcgcgcgg
     4021 gagagggccg gggtgagggc gctccctccc ccgctggggg agggttgggg tgggggccgg
     4081 cgcccgccca tgccggcggc gagatcggca gcgcccagta cttccaggcc ctggccgcgc
     4141 gcgccgtgcc ggatgtgcgc atgaatccgc tgctgctcaa gcccgaggcc gacacccgca
     4201 gccaggtggt gctgctgggg caggtcagcc acgagttgtc ggcgctgccc tggcgcgggc
     4261 gcagcgagcg cgtgtggccg cagatcgccg ccgcgctgga cgcgctgcgc gccgacaacg
     4321 acgtggtggt gatcgaaggt gccggctcgc cggccgagat caacctgatg gccagcgacg
     4381 tggtcaacct gcgcgtggcg caccatgccg acgcgcgctg cctgctggtg gccgacatcg
     4441 accgcggcgg cgcctttgcc cacctgtacg gcacctgggc gctgctgccc gaggccga
//
LOCUS       DAOQTZ010000168         4420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_22037_length_4420_cov_13.472394, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000168 DAOQTZ010000000
VERSION     DAOQTZ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22037_length_4420_cov_13.472394"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKN51_13150"
     CDS             <1..237
                     /locus_tag="PKN51_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT86090.1"
                     /translation="PRHPYTRMLLDAIPKMHDTGRARTPVQGEVPNPLNPPSGCTFHP
                     RCPHANARCKSERPALLTLKGIQVACHAVQEGRL"
     gene            383..1036
                     /locus_tag="PKN51_13155"
     CDS             383..1036
                     /locus_tag="PKN51_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor C family protein"
                     /protein_id="HNT86091.1"
                     /translation="MKPSILQITLASTLLAGLAQAAPFQNGSFEIGPAPGGSFITLGP
                     GNTSITGWEVTGAGIDYIGGLWQPADGSRSLDLSATAAGGIQQTFDTVAGHNYQVTFS
                     LAGNPSTPPLIKSVSVQATGSAATTYTFDISLSSTADMRWATQTYNFTATGAATTLSF
                     TSLDNSAMGPALDNVVVTDLTPATAVTPVPTLSEWALVLLATALGALGLRGRRGRGH"
     gene            1110..2057
                     /gene="tal"
                     /locus_tag="PKN51_13160"
     CDS             1110..2057
                     /gene="tal"
                     /locus_tag="PKN51_13160"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNT86092.1"
                     /translation="MNQLDALKQFTTVVADTGDFRQIAQYQPRDATTNPSLILKAVQK
                     PEYAPLLADTLARHRGKPLDEVVDRLLVRFGCEILSLIPGRVSTEVDARLSFDTEATV
                     TRAERLIELYQAEGVHIDRVLIKIASTWEGIRAAERLERRGIHCNLTLLFSFCQAVAC
                     GQARVQLISPFVGRIYDWYKKAAGSQWDEAAMAGPNDPGVRSVRRIYEHYKRFGIATE
                     VMGASFRNVGQITALAGCDLLTISPELMAQLAASPAPLARALDAEAARRLTLPAVQFD
                     EAGFRLALNQDAMATEKLAEGIRVFIQDAEKLEALMQAA"
     gene            2078..3682
                     /gene="pgi"
                     /locus_tag="PKN51_13165"
     CDS             2078..3682
                     /gene="pgi"
                     /locus_tag="PKN51_13165"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNT86093.1"
                     /translation="MDWRERARGDQTAVWPRLAAHGRQRLGAAGRFDLRQAFAEDAQR
                     VERFSLEAPPLLADLSKNLIDAPAQQLLTELAQACGVAAHRDAMLAGERINTSEARPV
                     THVHWRWASNRALAPEEQAPEAIKNIASVVPDASATLADMLSYAESVRADRGITDVVH
                     IGIGGSDLGPRLVVQALRAAGQASGPSLHFVANMDGHELASLLPRLTASRTLFIVVSK
                     TFGTAETLRNARSARDWFLAQGGSDVARHFVAVSANLAAARAFGAGRCFGFDDGLGGR
                     FSLWSPVGLAIAIAAGSAGFEALQAGARAMDRHFIQAPLARNLPVQLALLDLWYRNVL
                     GLASRCVAPYHHGLRSLPAYLQQLEMESNGKGVDLHGRPLAVASAPVTWGQAGSNGQH
                     AFFQMLHQGSDVVPVEFVLVARDAHGLPEHHAKLLANALAQSRALMLGQEGDAPERHF
                     PGNRPSTVLLLPDLSPHSLGALLALYEHRCFVLGSLWGINSFDQWGVELGKRHAGQIE
                     AALRGGALGPLDASTAALVAHLRGPA"
     gene            complement(3866..>4420)
                     /locus_tag="PKN51_13170"
     CDS             complement(3866..>4420)
                     /locus_tag="PKN51_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNT86094.1"
                     /translation="QERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEE
                     GIVAGGGVALLRARQAAGAIKGANADQDAGIKLVLKAIESPLREIVINAGDEASVVVN
                     KVLEGKGNFGFNAANGEYGDMIEMGILDPTKVTRTALQNAASVAGLMLTTEVMVAEAP
                     KDDAPAGGGMPGGMGGMGGMDGMM"
BASE COUNT          644 a         1604 c         1497 g          675 t
ORIGIN      
        1 ccgcgccacc cctacacgcg catgctgctg gacgccatcc ccaagatgca cgacaccggc
       61 cgcgcccgca ccccggtgca gggcgaagtg cccaacccgc tcaacccgcc cagcggttgc
      121 accttccacc cgcgctgccc gcacgccaat gcgcgctgca agagcgagcg accggccctg
      181 ctgacgctca agggcattca ggtggcctgc cacgcggtgc aagaggggcg actctgatcc
      241 gctctgcaaa ctgtagctgc tggcgcacac ggggtggtcg cctgcagcgt tttatgttcc
      301 aatccgcaag ccccgccgcg accccgaacg ctcggtcggt ggccacagtg ggatgtgtca
      361 accctcatca tcgaagattg tcatgaagcc atcgattctg cagatcaccc tggccagcac
      421 gctgctggcg ggcctggcgc aggccgcgcc cttccagaac ggcagttttg aaatcgggcc
      481 cgcgcccggt gggtcgttca tcacgctggg ccccggcaac accagcatca ccggctggga
      541 agtcacgggt gccggcatcg actacatcgg tggcctgtgg cagccggccg acggcagccg
      601 ctcgctggac ctcagcgcca ccgcggctgg cggcattcag cagaccttcg acacggtggc
      661 cggccacaac taccaggtga cgttctccct ggctggcaac ccgtccacac cgccgctgat
      721 caagagcgtc agcgtgcagg ccacgggcag cgccgccacc acctacacct tcgacatcag
      781 cctgtccagc acggccgaca tgcgctgggc cacgcagacc tacaacttca cggcgaccgg
      841 cgcagccacc acgctgagct tcaccagcct cgacaattcg gccatgggac cggcgctgga
      901 caacgtggtg gtgaccgacc tcaccccggc caccgcggtc accccggtgc ccaccctgtc
      961 ggaatgggcg ttggtgctgc tggccacggc gctgggcgcg ctgggcctgc gcggccgccg
     1021 cgggcgcggt cactgagcct ggcgggccgc cgccgcgtcg gtggtggccc gtgtcgtcgc
     1081 tcggggcgag cggggataat cgccccacca tgaaccagct cgacgccctc aagcaattca
     1141 ccacggtggt ggccgacacc ggcgacttcc ggcagatcgc gcagtatcag ccgcgcgacg
     1201 ccaccaccaa cccgtcgctg atcctgaagg cggtgcaaaa gcccgagtac gcgccgctgc
     1261 tggccgacac cctggcgcgt caccgcggta agccgctgga cgaagtggtc gatcggctgc
     1321 tggtgcgctt tggctgcgaa atcctgtcgc tcatcccggg ccgcgtgtcg accgaggtcg
     1381 atgcgcgcct gtcgttcgac accgaagcca cggtgacgcg cgccgagcgg ctgatcgagc
     1441 tgtaccaggc cgagggcgtg cacatcgacc gcgtgctgat caagattgcc agcacctggg
     1501 aaggcatccg cgccgccgag cggctggagc ggcgcggcat ccattgcaac ctgacgctgc
     1561 tgttctcgtt ctgccaggcg gtggcctgcg gtcaggcccg ggtgcaactg atttcgccct
     1621 tcgtcggccg catctacgac tggtacaaga aggccgccgg cagccagtgg gacgaagccg
     1681 ccatggccgg cccgaacgac ccgggcgtgc ggtcggtgcg ccgcatctac gaacactaca
     1741 agcgcttcgg cattgccacc gaggtgatgg gggcgagctt tcgcaacgtc ggccagatca
     1801 ccgcgctggc cggctgcgat ctgctcacca tcagccccga actgatggcg caactggccg
     1861 ccagcccggc gccgctggcg cgcgcgctgg acgccgaggc ggcacgccgg ctcacgctgc
     1921 cggccgtgca gtttgacgaa gcgggctttc gcctggcgct caatcaggac gccatggcca
     1981 ccgaaaagct ggccgagggc atccgcgtgt tcatccagga cgccgaaaag ctcgaagccc
     2041 tgatgcaggc ggcctgaccc cgccccgacc tgtgccgatg gactggcgag aacgcgcgcg
     2101 cggcgaccag acggcggtgt ggccgcggct ggcggcgcat gggcgccagc gcttgggcgc
     2161 tgccgggcgc ttcgatctgc ggcaggcctt cgccgaggac gcgcagcgcg tcgagcgttt
     2221 cagcctcgag gcgccgccgc tgctggccga tctgtcgaag aacctgatcg acgccccggc
     2281 gcagcagttg ctgaccgagc tggcgcaggc ctgcggcgtg gcggcgcacc gcgacgccat
     2341 gctggccggc gagcgcatca acaccagcga agcgcgcccg gtcacccacg tgcactggcg
     2401 ttgggcatca aatcgtgctc tggcgcccga ggagcaagcg ccagaagcta ttaaaaacat
     2461 agcatcggtg gtgcctgatg cgagcgccac gctggccgac atgctgagct atgccgagtc
     2521 cgtgcgtgcc gaccgcggta tcaccgacgt ggttcacatc ggcatcggcg gctccgacct
     2581 ggggccgcgc ctggtggtgc aggcgctgcg cgctgccgga caggccagtg gcccgagcct
     2641 gcacttcgtt gccaacatgg acgggcacga gctggccagc ctgctgccgc ggctgaccgc
     2701 ttcgcgcacg ctgttcatcg tcgtctccaa gaccttcggc accgccgaaa ccctgcgcaa
     2761 cgcgcggtcg gcgcgcgact ggttcctggc ccagggcggc agcgacgtgg cgcgccactt
     2821 cgtcgccgtc agcgccaacc tggcggccgc gcgcgccttc ggggccgggc gctgcttcgg
     2881 tttcgacgac ggcctgggcg ggcgcttttc gctctggtcg ccggtcggtc tggcgatcgc
     2941 catcgctgcc ggcagcgccg gcttcgaggc cctgcaggct ggcgcccgcg ccatggaccg
     3001 gcatttcatc caggcgccgc tggcgcgcaa cctgccggtg caactggcgc tgctggacct
     3061 gtggtatcgc aacgtcctcg gcctggccag ccgctgcgtg gcgccctacc accacggcct
     3121 gcgcagcctg cccgcctacc tgcagcagct ggagatggaa agcaacggca agggcgtcga
     3181 tctgcacggc cgccccctgg ccgtggccag cgcccccgtc acctgggggc aggctggcag
     3241 caacgggcag catgcgttct ttcagatgct gcaccagggc agcgacgtgg tgccggtgga
     3301 attcgtgctg gtggcgcgcg acgcccatgg cctgccggag caccacgcca agctgctggc
     3361 caacgcgctg gcgcagtccc gtgcgctgat gctggggcag gagggcgatg cgccggagcg
     3421 ccacttcccc ggcaaccggc cctccaccgt gctgctgctg cccgacctgt cgccgcacag
     3481 cctgggcgcg ctgctggcgc tgtacgagca ccgctgcttc gtgctgggct cgctgtgggg
     3541 catcaacagc ttcgaccagt ggggggtgga actgggcaag cgccacgccg gccagatcga
     3601 ggcggcgctg cgtggcggtg cgttggggcc cctggatgcg tcgacggcgg ctctggtggc
     3661 gcacctgcgc ggtccggcgt gaagcggtcg gcgccggcgg gggtgtgccg ctgctgatcc
     3721 ggccgccgcc tgcgcccgcc gcaccgcctg caccgcagtt gcgcggctca ggccccaaaa
     3781 aaaaagccgc tgtctccagc ggcttggctt gtcgggtggg caacgccgtt gcccacccac
     3841 aggcttcagg caggtcgggg gacgatcaca tcatcccgtc catgccgccc atgccgccca
     3901 tgccacccgg catgccgccg ccggccgggg cgtcgtcctt cggcgcttcg gcgaccatca
     3961 cctcggtggt cagcatcagg ccggccacgg aggcggcgtt ctgcagcgcg gtgcgggtga
     4021 ccttggtggg atccaggatg cccatctcga tcatgtcgcc atactcgccg ttggcggcgt
     4081 tgaagccgaa gttgcccttg ccttccagca ccttgttgac caccacgctg gcttcgtcgc
     4141 cggcgttgat gacgatttca cgcagcggcg actcgatggc cttgagcacc agcttgatgc
     4201 cggcgtcctg gtcggcgttg gcacccttga tggcgccggc cgcctggcgg gcgcgcagca
     4261 gggccacgcc accgccggcg acgatgcctt cttccaccgc agcgcgggtg gcgtgcaggg
     4321 cgtcttccac gcgggctttc ttctccttca tctcgacctc ggtggcggcg cccaccttga
     4381 tgacggccac gccgccggcc agcttggcca cgcgctcctg
//
LOCUS       DAOQTZ010000169         4317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_22769_length_4317_cov_12.795870, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000169 DAOQTZ010000000
VERSION     DAOQTZ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22769_length_4317_cov_12.795870"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PKN51_13175"
     CDS             complement(<1..350)
                     /locus_tag="PKN51_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HNT86095.1"
                     /translation="MNALSIADHIHTLGLQARTASAQMARAPAAIKNKALRALARRLR
                     DGVQALQTDNAQDLERAVAAGLSAPMVDRLRLTPQVIETCAQGCEQLAAMPDVIGDIV
                     GMRQQPSGIRVGQMR"
     gene            complement(416..1483)
                     /gene="holA"
                     /locus_tag="PKN51_13180"
     CDS             complement(416..1483)
                     /gene="holA"
                     /locus_tag="PKN51_13180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNT86096.1"
                     /translation="MQLAAAQLARHLQQGLRPLYVLHGDEALLVQEAADAIRATARAQ
                     GHTERSVHTVAGAHFDWGAVLAAGSSLSLFADRQLVEIRIPSGKPGKDGSAALQQLAE
                     AAPGNDGVLTLVLLPRLDKATRTSAWFAALDGAGVTVQIDGIERAALPLWLAQRLAAQ
                     GQRVAAGEEGQRTLAFFADRVEGNLLAAHQEISKLALLHPPGELSWAQVEAAVLNVAR
                     YDVFKLSEAVLGGQPARVQRMLDGLQAEGEAEVLVHWALAEDIRALQRVRDALDAGKP
                     LPMALREHRIWGPRERLFERVLPRLSAATASRLLHSAHVVDGIVKGLKAPDWPHDGWQ
                     ALHRLAMQLCRACMPAAPRPR"
     gene            complement(1485..1985)
                     /gene="lptE"
                     /locus_tag="PKN51_13185"
     CDS             complement(1485..1985)
                     /gene="lptE"
                     /locus_tag="PKN51_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNT86097.1"
                     /translation="MRPTRRTLLLASVPLLLSGCGFALRQAPQFGFRSLYLALPAHSA
                     LALELRRQLEGSGSLQVFTDAAQRDKAEVVLESPGEQRERAVVSVSAGGEVREFQLRI
                     RLRFRLRAADGRELLPMSEILRQIDQSYSESAALSKEQEALMLYQNMQSDIVQQVMRR
                     LATVRL"
     gene            complement(2003..>4317)
                     /gene="leuS"
                     /locus_tag="PKN51_13190"
     CDS             complement(2003..>4317)
                     /gene="leuS"
                     /locus_tag="PKN51_13190"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNT86098.1"
                     /translation="CDPEYYRWNQWLFLKMLEKGIAYRKTQVVNWDPVDQTVLANEQV
                     IDGRGWRTGALVEKREIPGYYLKITDYAQELLDHVQIGNPKATLAGWPERVRLMQENW
                     IGKSEGVRFAFTHDIRGADGAPIQGGRMYVFTTRADTIMGVTFCAVAPEHPLAAHAAR
                     DNPALAAFIEQCKQGGTTEAELAARDKEGLPTGLTVTHPLTGEAVPLWVGNYVLMGYG
                     DGAVMGVPAHDERDFAFALKYRLPIQQVIHVDGQSYDDGRWQDWYADKQRGVTINSEN
                     FSGLGHQDAVDAVAQALAAKGLGEKKTTWRLRDWGISRQRYWGTPIPIIHCPACGVVP
                     VPEQDLPVVLPQDLVPDGSGNPLNKCEAFLQVACPCCGQPARRETDTMDTFVDSSWYF
                     MRYCDPTNHRQMVAEGSRYWMPMDQYIGGIEHAILHLLYARFWTKVMRDLGLVQIDEP
                     FTRLLTQGMVLNHIYSRRNDKGGIEYFWPADVEPVFDADGHQVGARLNKAVGALPAGT
                     PIDYEGVGTMSKSKNNGVDPQELIDRYGADTARLYTMFTAPPEATLEWNDAAVEGSYR
                     FLRRVWNFGVKLNATENIAARAVLASAAAQNDLKLSKAAKALRHEIHSVLGQIDYDYQ
                     RLQYNTVVSGAMKMLNALEGFNPDAAERGDQAVLAEGFSKLLRVLYPVTPHLTHVLWQ
                     ELGFAAVHGDLLDAPWPRVDPEALRQDEIELVLQINGKLRGAVAVPAGADQARIEQLA
                     LASEAFARHASGTVRKVVVVPGRLVNIVVA"
BASE COUNT          573 a         1509 c         1535 g          700 t
ORIGIN      
        1 cgcatctgcc ccacgcggat gccgctgggc tgctggcgca tgcccacgat gtcgccgatc
       61 acgtcgggca tggccgccag ctgctcgcag ccctgggcgc aggtttcgat cacctgcggg
      121 gtcaggcgca ggcgatcgac catgggcgcg ctcaggccgg ccgccacggc gcgctccagg
      181 tcctgggcgt tgtcggtctg cagcgcctgc acgccgtcgc gcaggcgccg cgccagcgcc
      241 cgcaatgctt tgtttttaat agctgccggc gcacgcgcca tctgcgccga agccgttctg
      301 gcctgcaaac caagggtgtg gatgtggtcg gcgatggaaa gcgcgttcat ggtgtgatgg
      361 tacggcagat ggggtgtgag ggcggcggcg cggcgcccgc ggtgcgcgcg ccggttcagc
      421 gcgggcgcgg cgcggcaggc atgcaggcgc ggcacagctg catcgccagc cgatgcaggg
      481 cctgccagcc gtcgtgcggc cagtcgggcg ccttcaggcc cttgacgatg ccgtccacca
      541 catgggccga gtgcagcagg cggctggcgg tggcggcgct caggcgcggc agcacgcgct
      601 cgaacaggcg ctcgcgcggg ccccagatgc ggtgttcgcg cagcgccatc ggcagcggct
      661 tgccggcgtc cagcgcgtcg cgcacgcgct gcagggcgcg gatgtcttcg gccagcgccc
      721 agtgcaccag cacctcggcc tcgccctcgg cctgcaggcc gtcgagcatg cgctgcacgc
      781 gcgccggctg gccgcccagc accgcttcgg acagcttgaa cacgtcgtag cgcgccacgt
      841 tcagcacggc ggcctcgacc tgggcccagg acagctcgcc cggcgggtgc agcagggcca
      901 gcttgctgat ttcctggtgc gcggccagca ggttgccttc cacccgatcg gcaaagaagg
      961 ccagggtgcg ctggccttcc tcgcccgccg ccacgcgctg gccctgggcg gccaggcgct
     1021 gggccagcca cagcggcagc gcggcgcgct cgatgccgtc gatctgcacc gtcacgccgg
     1081 cgccgtcgag cgcggcaaac caggcgctgg tgcgggtggc cttgtccagc cgcggcagca
     1141 gcaccagggt cagcacgccg tcgttgccgg gcgcggcctc ggccagctgc tgcagcgccg
     1201 cgctgccgtc cttgcccggc ttgcccgagg ggatgcggat ttcgaccagc tgccggtcgg
     1261 caaacaggct caggctgctg ccggcggcca gcaccgcgcc ccagtcgaaa tgcgcgccgg
     1321 cgacggtgtg cacgctgcgc tcggtgtggc cctgggcgcg cgcggtggcg cggatggcgt
     1381 cggccgcctc ctgcaccagc agggcctcgt cgccgtgcag cacgtacagc gggcgcaggc
     1441 cctgctgcag gtgccgggcc agttgcgcgg ccgccaactg catctcagag cctcacggtc
     1501 gccagccgcc gcatgacctg ctgcacgatg tcgctctgca tgttctggta gagcatcagc
     1561 gcctcctgtt ccttggacag cgcggccgat tcgctgtagc tctggtcgat ctggcgcagg
     1621 atttccgaca tcggcagcag ctcgcgcccg tcggcggcgc gcaggcgaaa gcgcaggcgg
     1681 atgcgcagct ggaattcgcg cacctcgccg ccggccgaca cgctgacgac ggcgcgctcg
     1741 cgctgctcgc ccgggctttc cagcaccacc tcggccttgt cgcgctgcgc ggcgtcggtg
     1801 aagacctgca ggctgccgct gccttcgagc tggcggcgca gctccagcgc cagcgccgaa
     1861 tgggccggca gcgccaggta caggctgcga aagccgaact gcggcgcctg ccgcagcgca
     1921 aagccgcagc cgctcagcag cagcggtacg ctggccagca gcagggtacg gcgtgtgggg
     1981 cgcatggcgc tgtcaccgcc tctcaggcca ccacgatgtt gaccagccgg ccgggcacca
     2041 ccaccacctt gcgcaccgtg ccgctggcgt ggcgcgcaaa ggcttcgctg gccagcgcca
     2101 gttgctcgat ccgggcctgg tcggcgccgg cgggcacggc gacggcgccg cgcagcttgc
     2161 cgttgatctg cagcaccaac tcgatttcgt cctgcctgag cgcctccggg tcgacgcgcg
     2221 gccagggcgc gtccagcagg tcgccatgca ccgcggcaaa gccgagctcc tgccacagca
     2281 cgtgcgtgag gtgcggcgtg accgggtaga gcacgcgcag cagcttggaa aagccttcgg
     2341 ccagcaccgc ctggtcgccg cgttcggcgg cgtcggggtt gaagccttcc agcgcgttga
     2401 gcatcttcat ggcgcccgac accacggtgt tgtactgcag gcgctggtag tcgtagtcga
     2461 tctgccccag cacgctgtgg atctcgtgcc gcagggcctt ggcggccttg ctcagtttca
     2521 ggtcattctg ggccgcagcg cttgccagaa ctgcgcgagc agctatgttt tcagtagcat
     2581 tcagcttgac gccgaagttc cacacccggc gcaggaagcg gtagctgcct tccaccgcgg
     2641 cgtcgttcca ctccagcgtg gcctcgggcg gggcggtgaa catggtgtac aggcgcgcgg
     2701 tgtcggcgcc gtagcggtcg atcagctcct gcggatcgac gccgttgttc ttggacttgg
     2761 acatggtgcc cacgccctcg tagtcgatcg gcgtgccggc cggcagggcg cccacggcct
     2821 tgttcagcct ggcgccgacc tggtggccgt cggcgtcgaa caccggctcg acgtcggccg
     2881 gccagaagta ttcgatgccg cccttgtcgt tgcggcgcga atagatgtgg ttgagcacca
     2941 tgccctgcgt cagcaggcgc gtgaagggct cgtcgatctg caccagcccc agatcgcgca
     3001 tcaccttggt ccagaaacgt gcgtacagca ggtgcaggat ggcgtgctcg atgccgccga
     3061 tgtactggtc catgggcatc cagtaccggc tgccttcggc caccatctgg cggtggttgg
     3121 tcgggtcgca gtagcgcatg aagtaccagg acgaatcgac gaaggtgtcc atggtgtcgg
     3181 tttcgcgccg cgccggctgg ccgcagcagg ggcaggccac ctgcaggaag gcttcgcact
     3241 tgttcaacgg gttgccgctg ccgtcgggca ccaggtcctg cggcagcacc accggcaggt
     3301 cctgctcggg caccggcacc acgccgcagg ccgggcagtg gatgatgggg atcggcgtgc
     3361 cccagtagcg ctggcgcgag atgccccagt cgcgcaggcg ccaggtggtt ttcttctcgc
     3421 ccagaccctt ggcggccagc gcctgcgcca ccgcgtccac ggcgtcctgg tgccccaggc
     3481 cgctgaagtt ctcggagttg atggtgacgc cgcgctgctt gtcggcgtac cagtcctgcc
     3541 agcggccgtc gtcgtagctc tggccgtcga cgtggatcac ctgctggatc ggcaggcggt
     3601 acttgagggc gaaggcgaag tcgcgctcgt cgtgcgccgg cacgcccatc acggcgccgt
     3661 cgccatagcc catcagcacg tagttgccga cccacagcgg caccgcctcg ccggtcagcg
     3721 ggtgggtcac cgtcaggccg gtgggcaggc cttccttgtc cctggcggcc agttcggcct
     3781 cggtggtgcc gccctgcttg cattgctcga tgaaggcggc cagcgcgggg ttgtcgcgcg
     3841 ccgcgtgcgc cgccagcggg tgctcgggcg cgacggcgca gaaggtcacg cccatgatgg
     3901 tgtcggcgcg cgtggtgaag acgtacatgc gcccgccctg gatcggcgcg ccgtccgcgc
     3961 cgcggatgtc gtgcgtgaag gcgaagcgca cgccctcgct cttgccgatc cagttttcct
     4021 gcatcaggcg cacgcgctcg ggccagccgg ccagcgtcgc cttggggttg ccgatctgca
     4081 cgtggtcgag cagttcctgc gcgtagtcgg tgatcttgag gtagtagccc gggatctcgc
     4141 gcttttccac cagcgcgccg gtgcgccagc cgcggccgtc gatcacctgc tcgttggcca
     4201 gcacggtctg gtccaccggg tcccagttga cgacctgggt cttgcggtag gcgatgccct
     4261 tctccagcat cttcaggaac agccactggt tccagcggta gtactcgggg tcgcagg
//
LOCUS       DAOQTZ010000170         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_23743_length_4190_cov_15.657557, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000170 DAOQTZ010000000
VERSION     DAOQTZ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23743_length_4190_cov_15.657557"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKN51_13195"
     CDS             complement(<1..222)
                     /locus_tag="PKN51_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT86099.1"
                     /translation="MKPSTDPNDALMARARRVIPGGVNSPVRAFRAVGGTPRFVQRAQ
                     GPYFWDAAGTRYIDYIGSWGPMILGHGHPA"
     gene            complement(206..1078)
                     /locus_tag="PKN51_13200"
     CDS             complement(206..1078)
                     /locus_tag="PKN51_13200"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNT86100.1"
                     /translation="MPNVLSFNAGDPTGAGGSGSDATVIAAIGAHALPVVTGVYVRDT
                     RETMSHCAIDEAAVAEQAAAVAEDVPLHVIKVGFVGSAEAVSAIAAFTSDYASVPVVA
                     YMPDLSWWSEAEIDHYLDACRELLLPQTTVLVGNNATLRHWLLPESTTDRHARATDLA
                     RAAAERGVAFVLVTGLPLAGDRLGNVLASPEAELFSQSFERIDADFLGAGDILSAALA
                     ALIATGSELTEATSEALQYLDQALDHGFRPGMGRALADRLFWAASDADDQADTADDGP
                     ALPPFPEPFNETKH"
     gene            1251..1421
                     /locus_tag="PKN51_13205"
     CDS             1251..1421
                     /locus_tag="PKN51_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNT86101.1"
                     /translation="MNPKTWMCVICGWLYDEAAGAPDHGIAPGTPWEQVPADWCCPEC
                     GAHKLDFEMVQI"
     gene            1501..1887
                     /locus_tag="PKN51_13210"
     CDS             1501..1887
                     /locus_tag="PKN51_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT86102.1"
                     /translation="MTSRRVLVIDDSNTIRRSAEIFLKQGGHEVMLAEDGFDALAKVN
                     DAQPDLIFCDILMPRLDGYQTCAIIKRNEKYSSIPVVMLSSKDGVFDKARGRMVGSQD
                     YLTKPFTKDQLLQAVQQFGNANQGAV"
     gene            1887..2252
                     /locus_tag="PKN51_13215"
     CDS             1887..2252
                     /locus_tag="PKN51_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT86103.1"
                     /translation="MSIRNVLIVDDSKTELLHLSEMLKKAGYAVSTAENAEEALRRLE
                     QAKPDLILMDVVMPGQNGFQLTRAISRDPRYADVPIIMCTSKNQETDRVWGMRQGARD
                     YVTKPVDQAELMGKIRALG"
     gene            2258..2791
                     /locus_tag="PKN51_13220"
     CDS             2258..2791
                     /locus_tag="PKN51_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNT86104.1"
                     /translation="MSNKQALREFQSRLAARLQSAQTGGVAASWLAVEAGSQRLLFPL
                     SHAGEIFSWTDVQRVPYVQPWFLGVANLRGGLSGVIDLARFVGGAEPVAGRSEADLAQ
                     CRLVALNPVLGANCALLVDRLLGLRTTEAFASSESADPIAPAFFGHRYADADGQSWQE
                     LNLQLLSQHPAFLGIGV"
     gene            2813..>4190
                     /locus_tag="PKN51_13225"
     CDS             2813..>4190
                     /locus_tag="PKN51_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNT86105.1"
                     /translation="MALASQLTNLFRSKSQDSSTGGDEAAVHEELLSTPPTSHADDSR
                     AAAESSVMVSAIDDSDDGATPGDDVFSVPLLGTRTAAQHQRLLSILLGLALFALVIVG
                     FWTVSRTDKGARQVGAIGQSLMQSQRLAKSVSQALVGSPEAFGEVKESAAALSANVRG
                     LQDGSDVVPALSDAFSDELGKIGPLAQRADKSAAVVMAQQQILTQVGAALRAVSRQST
                     ELLEVAETVSSLRLQQNASVSEISAAGQLVMLTQRIGKSANEFLTIEGVSPEAVFLLG
                     KDLNTFKEIAEGLLNGSADLRLAPARDPQTRERLTELLKLYEETRTQAGGVLGNLQGL
                     VAAREAQTTIVSDSEPLRKALEGLQDKLSGTSGVGFGNLMMLVIPAILLLLAAAGLVA
                     VQLRDSRKRQTLAEQQRRSAEQDQQEAKRVNDANQAAILRLMNELQTVAEGDLTQEAT
                     VTEDITGAI"
BASE COUNT          746 a         1260 c         1431 g          753 t
ORIGIN      
        1 ggccgggtgg ccgtggccca ggatcatcgg gccccaggag ccgatgtagt cgatgtagcg
       61 tgtgcccgcc gcgtcccaga agtaggggcc ctgggcgcgc tgcacgaagc gcggcgtgcc
      121 gccgacggcg cgaaaggccc gcacgggcga gttgacgccg ccgggaatca cgcggcgggc
      181 gcgcgccatc agcgcgtcgt tggggtcagt gcttggtttc attgaagggt tccgggaaag
      241 ggggaagggc tgggccgtcg tcggcagtgt ccgcctggtc gtcggcatcg gaggcggccc
      301 aaaacagccg gtcggccagc gcccggccca tgccggggcg aaagccgtgg tccagggcct
      361 ggtccaggta ttgcagggct tcgctggtcg cttcggtgag ctcgctgccg gtggcgatca
      421 gcgccgccag cgccgccgag aggatgtcgc cggcacccag aaaatcggca tcgatgcgtt
      481 cgaagctctg gctgaacaac tcggcctcgg gcgacgccag cacgttgccc agtcggtcgc
      541 cggccagcgg cagcccggtc accagcacga acgccacgcc gcgctcggcc gccgcccgtg
      601 ccaggtcggt ggcgcgcgca tggcggtcgg tggtggattc gggcagcagc cagtggcgca
      661 gcgtggcgtt gttgccgacc agcacggtgg tctgcggcag cagcagctcg cggcacgcgt
      721 ccaggtagtg gtcgatttcg gcttcggacc accacgacag gtcgggcatg taggccacca
      781 ccggcaccga cgcgtagtcc gaggtgaagg cggcaatggc cgacaccgcc tcggcactgc
      841 ccacgaaacc caccttgatc acgtgcaacg gcacgtcctc ggccacggcg gccgcctgtt
      901 cggccacggc cgcctcatcg atggcgcagt ggctcatggt ttcgcgcgtg tcgcgcacgt
      961 acacgccggt gaccaccggc agcgcatggg cgccgatggc ggctatcacc gtggcatcgc
     1021 tgcccgagcc gccggcaccg gtggggtctc cggcgttgaa gctcaggacg ttgggcaaac
     1081 tgtcgtattc agccaacaaa tctggcgcgg tcgcgccgcc ggacgcatcg gccggtgtgg
     1141 aactgctcat aggacacaca ttggcgggcc gactgtgaca gagtgtcgta tgtaggcgaa
     1201 agccgtcgat acaatcgatt gcattctagg taattgcaac ttaagctgta atgaatccca
     1261 agacttggat gtgcgtgatc tgcggttggc tttacgacga agccgcggga gcacctgatc
     1321 atggtatcgc gccaggcacg ccttgggagc aggttccggc cgactggtgc tgcccagagt
     1381 gcggcgccca caagctggat tttgaaatgg tgcagatctg agcctgcgaa atgcgtggtc
     1441 tgtatggacg gcaaagccgg gggcgtccag gggcgtccac gctacaaccg agagggtgcg
     1501 atgacatcaa ggcgggtgct ggtcattgac gacagcaaca ccattcggcg cagcgccgaa
     1561 atcttcctca agcagggagg gcacgaggtc atgctggccg aagacggttt tgacgccctg
     1621 gccaaggtca acgacgccca gcctgacctg atcttctgcg acatcctgat gccgcggctg
     1681 gatggctatc agacctgtgc catcatcaaa cgcaacgaaa agtattcatc catacccgtg
     1741 gtcatgttgt cgtccaagga tggtgtgttc gacaaggcgc gtggccgcat ggtcggctcg
     1801 caggactacc tgaccaaacc cttcaccaag gatcaactgc tgcaggcggt gcagcagttc
     1861 ggaaacgcca atcaaggagc cgtgtgatgt cgatccgcaa cgtgctgatc gtggacgatt
     1921 cgaagactga gttgctgcac ctgagcgaaa tgctcaagaa ggcgggctac gccgtgagca
     1981 ccgcagaaaa cgccgaggag gccctgcgcc gtctggagca ggccaagccc gatctgattc
     2041 tgatggacgt cgtgatgccg ggccagaacg gttttcagct cacgcgcgcc atttcgcgcg
     2101 atccgcgcta cgccgacgtg ccgatcatca tgtgcaccag caagaaccag gaaaccgacc
     2161 gggtctgggg catgcgccag ggtgcgcgcg attacgtcac taagccggtc gaccaggccg
     2221 agctgatggg caagatcaga gccttgggct gatcgacatg tccaacaagc aggcactgcg
     2281 cgaattccag agccgtctgg cggcgcggct gcagtcggcc cagaccgggg gcgtggcggc
     2341 gtcgtggctg gcggtggaag ccggatcgca gcgtctgctg tttccgctca gtcatgcggg
     2401 cgaaatcttc tcctggaccg acgtgcagcg cgtcccttac gttcaacctt ggtttttggg
     2461 tgtggccaat ctgcgcggtg ggttgtcggg cgtgatcgat cttgcgcgtt tcgtcggcgg
     2521 cgcagaacct gtggcgggcc gcagcgaggc ggatctcgcg caatgtcgtc tggtggcgct
     2581 caatccggtg cttggcgcca actgcgcgtt gctggtcgat cgcctgttgg gattgcgcac
     2641 caccgaagcc tttgcgtcct cggagtcggc agacccgatc gcgcctgcgt ttttcgggca
     2701 ccggtacgcc gatgccgatg gtcagtcctg gcaggagttg aacctgcagc ttctctcgca
     2761 gcacccggcc tttctgggta tcggggtgta atttgtctta cggggtcacg acatggcact
     2821 cgcttctcaa ctcaccaatc tgttccgctc caagtctcag gattcctcaa ctggcggtga
     2881 cgaggccgcg gttcatgaag agctgctgag cacgccgcca acgtcgcatg ctgacgattc
     2941 gcgcgccgca gcggagagtt cggtgatggt ctcggcaatc gatgattccg acgacggcgc
     3001 tacgcccgga gacgatgtgt tttccgtccc cctgctgggt acccggacgg cggcacagca
     3061 tcagcgcctg ctttcgatac tgctgggact ggcgttgttc gccctggtga tcgtgggttt
     3121 ctggacggtg tcgcgcaccg acaagggtgc gcgccaggtg ggtgcgatag gtcagtcgct
     3181 gatgcagtcg caacgtttgg ccaaatcggt atcacaggcg ctggtcggca gtcccgaggc
     3241 tttcggtgag gtgaaggaaa gcgccgccgc cttgtcggcc aacgtgcgag gcctgcagga
     3301 tggcagtgat gtggtgcctg cgctgagcga cgccttcagt gacgagctgg gcaagatcgg
     3361 gccgctggct cagcgtgctg ataagagtgc ggcggtcgtg atggcgcagc agcagatcct
     3421 gacccaggtc ggtgccgccc tgcgcgcggt cagtcgacag tccaccgagt tgctggaagt
     3481 ggccgaaacc gtgtcgtcgc tcaggttgca gcagaacgca tccgtcagtg agatttccgc
     3541 ggcagggcaa ctggtgatgc tgacgcagcg tatcggcaag tcggccaatg aattcctgac
     3601 catcgagggc gtgagcccag aggcggtgtt cctgctgggt aaggacctca ataccttcaa
     3661 ggagatcgcc gaggggctgc tcaacggcag cgcagacttg cgtctggcgc cggcccgtga
     3721 tccgcagact cgcgaacgtc tgacggaact gctcaagctt tacgaggaaa cccgcactca
     3781 ggccggcggc gtgctgggca acctgcaggg tctggtcgca gcacgtgaag cgcagaccac
     3841 catcgtgtcc gacagcgagc cgttgcgcaa ggcactggaa ggtctgcagg acaagttgtc
     3901 gggcacctcg ggtgtcggat tcggcaacct gatgatgctg gtcattccgg cgattctgct
     3961 gttgctggct gccgctggcc tggttgccgt gcaactgcgc gatagtcgca agcgccagac
     4021 gctggctgag cagcagcgtc gttccgccga gcaggatcag caggaagcca agcgcgtcaa
     4081 tgacgccaat caggcggcca tcttgcggct catgaacgaa ctgcagacgg tggctgaagg
     4141 cgatctgacc caggaagcca cggtgaccga ggacatcacc ggcgccatcg
//
LOCUS       DAOQTZ010000171         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_23893_length_4170_cov_16.000729, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000171 DAOQTZ010000000
VERSION     DAOQTZ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23893_length_4170_cov_16.000729"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PKN51_13230"
     CDS             <1..1326
                     /locus_tag="PKN51_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNT86106.1"
                     /translation="PYIFRGALDCGASTISKQMEVAAVYAIAGLAQAEQSEVVARAYA
                     GEKLAFGPEYLIPKPFDPRLMMKIAPAVAEAAAASGVATRPIVDLDAYRERLQTFVYA
                     SGTTMKPIFDAARAAASKRVAYAEGEEERVLRAAQIVVDEGLARPTLIGRPAIIAQRI
                     VKFGLRLAEGADYDVVNVENDVRYKGFWQEYYELTKRRGMTVPMAKIEMRRRLTLIGT
                     MLLRAGQVDGLICGTWGTIPMHLNYVDQVIGKRPGTHTYAAMNGVLLPGRQLFMVDTH
                     INVDPTAEQLTEITIAAAEQVQRFGLKPRAALLSHSNFGSANDASAVKMREVLERVHA
                     QAPWLEIDGEMHGDVALDSGMRRALMPDSTLKGDANLLILPGIEAANIAYNLLKTAAG
                     GNIAIGPVLLGAAKPVHILTASATVRRIVNMTALAVADASAANRHQSNK"
     gene            1546..1881
                     /locus_tag="PKN51_13235"
     CDS             1546..1881
                     /locus_tag="PKN51_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNT86107.1"
                     /translation="MAGVAQARGLSWGVEPATVAVAELPSQGQHTYRLILQGGPFPHD
                     KDGSVFGNRERLLPRERRGYYREYTVETPRARGRGARRIVCGGQRPTAPEACYYTSDH
                     YSSFRKIVQ"
     gene            1962..2387
                     /locus_tag="PKN51_13240"
     CDS             1962..2387
                     /locus_tag="PKN51_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNT86108.1"
                     /translation="MDQPLRQPQDVPLRNVRSNIVQSIRAFHAHDLQQAAAQLGQHFL
                     YANLAHAQTKQDVLDTIAAQFTFPAHFGKNFDALYDCMTDPLHKSGAQPGFVVVLEHI
                     PATAKFDKEAREQLLDIFRDAADYWGDRKIPFRCFYSFL"
     gene            2691..>4170
                     /locus_tag="PKN51_13245"
     CDS             2691..>4170
                     /locus_tag="PKN51_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT86109.1"
                     /translation="MSAEATELLPKQELQAQVVQALQAVLPAHSLLWTDEQTTPYECD
                     GLTAYRQRPLAVALPDTAEQVREVLRVCHRLGVPVVARGAGTGLSGGALPHERGVTLS
                     LARFNRILAIDPLARTARVQCGVRNLAISEAAAAHGLYYAPDPSSQIACTIGGNVAEN
                     SGGVHCLKYGLTVHNVLQVSGFTVEGEPIEFGSQALDAPGYELLALVHGSEGMLAVTT
                     EVTVRLLPRPRLARCIMASFDDVRRAGEAVAAVIAAGIIPAGLEMMDKPMTAAVEDFV
                     HAGYDLQAAAILLCESDGTPEEVEEEIQRMSQVLSAAGATRIAVSQSEAERLKFWSGR
                     KNAFPASGRISPDYMCMDSTIPRKRLADILLAIGQMEQKYGLGCCNVFHAGDGNLHPL
                     ILFDANDPDQLHRAELFGADILETSVAMGGTITGEHGVGVEKLNSMCVQFSAAENEQM
                     FGVKRAFDPAGLLNPGKLIPTLNRCAEYGKMLVRGGRLPFPEL"
BASE COUNT          726 a         1417 c         1386 g          641 t
ORIGIN      
        1 ccctacatct tccgcggcgc cctcgattgc ggtgccagca ccatcagcaa gcagatggag
       61 gtcgcggccg tgtacgccat cgccggcctg gcgcaggccg agcagagcga agtggtggcg
      121 cgtgcctacg ccggtgaaaa gctggccttc ggccccgaat acctgatccc caagccgttc
      181 gacccgcgcc tgatgatgaa gatcgcgccg gcggtggccg aagcggcggc ggccagcggc
      241 gtcgccacgc ggccgatcgt cgatctggac gcctaccgcg aacgcctgca gaccttcgtc
      301 tacgcctcgg gcacgacgat gaagccgatc ttcgacgccg cccgcgccgc cgccagcaag
      361 cgtgtggcct acgccgaggg cgaggaagag cgcgtgctgc gcgcggcgca gatcgtggtt
      421 gacgaggggc tggcacggcc caccctgatc ggccgtcccg ccatcatcgc ccagcgcatc
      481 gtcaaattcg gcctgcgcct ggccgaaggc gccgattacg acgtcgtcaa cgtcgagaac
      541 gacgtgcgct acaagggctt ctggcaggaa tactacgaac tgaccaagcg ccgcggcatg
      601 acggtgccga tggccaagat cgagatgcgc cgccgtctga ccctgatcgg caccatgctg
      661 ctgcgcgccg gccaggtgga cggcctgatc tgcggcacct ggggcaccat ccccatgcac
      721 ctgaactatg tggaccaggt gatcggcaag cgccccggca cccacaccta tgccgccatg
      781 aacggcgtgc tgctgcccgg caggcagctg ttcatggtcg acacccacat caacgtcgat
      841 ccgaccgccg agcagctgac cgagatcacc atcgcggcgg ccgaacaggt gcagcgcttt
      901 ggcctcaagc cgcgggcggc gctgctgtcg cattccaact tcggttctgc caacgacgcc
      961 agcgcggtca agatgcgcga agtgctggag cgggtgcacg cccaggcgcc ctggctggaa
     1021 atcgacggcg agatgcacgg cgacgtggcg ctcgacagcg gcatgcgccg cgcgctgatg
     1081 cccgacagca cgctcaaggg cgacgccaac ctgctgatcc tgcccggcat cgaggccgcc
     1141 aacatcgcct acaacctgct caagaccgcc gccggcggca acatcgccat cggcccggtg
     1201 ttgctgggcg ccgccaagcc tgtgcacatt ctcacggcca gcgccaccgt gcgccgcatc
     1261 gtcaacatga cggcactcgc ggtggccgac gccagtgccg ccaacaggca tcaatcaaat
     1321 aagtaagtac acgctacctt acgaatttca ccccgctggc gctgcctgat ttttaggcag
     1381 gcgcttgctt tttggtgacg catgcgtcac actagcgcct tggaattttc gggttaaccc
     1441 ggggtttccg aaagggtgtc ttccatgtgg cgaacagcgg cgtccggtgc tgcccgcgtg
     1501 tcccggttgg ttttggcagc ttcggtgctg acggtcgcac tccccatggc gggcgtggcc
     1561 caggcacgcg ggctgtcctg gggggtcgag ccggccaccg tggcggtggc cgaactgccg
     1621 tcccagggcc agcacaccta ccggctgatt ctgcaaggcg ggccatttcc tcacgacaag
     1681 gacgggtcgg tgttcggcaa ccgggagcgt ctgctgccgc gcgagcggcg cggctactac
     1741 cgcgaataca ccgtcgaaac cccgcgcgcg cgcggccgtg gtgcacggcg catcgtctgc
     1801 ggcggccagc gcccgaccgc acccgaggcc tgttattaca ccagcgatca ctattcgagc
     1861 ttcaggaaga tcgtgcaatg aaggcgtttg aacagtttca gcccgcgccc gccgtggcgg
     1921 tgggcgccga ccggttattg acttcagaga aagcagcggg gatggatcaa ccacttcgtc
     1981 aaccccagga tgtgccgctc aggaatgtgc gcagcaacat cgtgcagtcg atccgcgcgt
     2041 tccacgcgca cgacctgcag caggcggcgg cccagctggg ccagcatttc ctgtacgcca
     2101 acctggccca tgcccagacc aagcaggacg tgctggacac gatcgcggcg cagttcacct
     2161 tcccggccca cttcggcaag aacttcgacg ccctgtacga ctgcatgacc gacccgctgc
     2221 acaagagcgg cgcgcagccc ggcttcgtgg tggtgctgga acacatcccg gccaccgcca
     2281 agttcgacaa ggaggcgcgc gagcagctgc tcgacatctt ccgcgacgcc gccgactact
     2341 ggggtgaccg caagatcccg ttcaggtgct tctattcttt tctgtagccc gttctgcaac
     2401 cactggccag gcagaacggg cgaacgaggc ccagggcgaa ccggcgcccc agggcatcga
     2461 ggccgacgat ggcgaaaaaa tgccgaccga caagctggtc gacatctctc cgctggcgct
     2521 gcgcatgagc agcccgttca acgcgggcta ctggctcagc gccgcctgaa accgatcgcc
     2581 gatcgcccaa gcccgccgtg tgcgggcatt tttgttggcc attggctggc aggccgattt
     2641 ccgggtcatc gcctatagtc ggcagcaggg atcgccccag gagccacacc atgtccgccg
     2701 aagccaccga attgctacca aaacaagagc tgcaggcgca agtggtacaa gcgctacaag
     2761 ccgttttgcc tgcgcattcg ctgctgtgga cggatgagca gaccacgccc tacgagtgcg
     2821 acggcctgac cgcctatcgc cagcggccgc tggcggtggc gctgcccgac acggcggagc
     2881 aggtgcgcga ggtgctgcgc gtgtgccacc gcctgggtgt gccggtggtg gcgcgcggcg
     2941 ccggcaccgg gctgtcgggc ggcgcgttgc cgcacgagcg gggggtgacg ctgtcgctgg
     3001 cgcgcttcaa ccgcatcctg gccatcgacc cgctggcgcg cacggcgcgc gtgcagtgcg
     3061 gggtgcgcaa cctggccatc agcgaggcgg cggccgccca tggcctgtac tacgcgcccg
     3121 acccgtccag ccagatcgcc tgcaccatcg gtggcaacgt ggccgagaac tccggcggcg
     3181 tgcactgcct gaaatacggc ctgacggtgc acaacgtgct gcaggtcagc ggcttcacgg
     3241 tggaaggcga accgatcgaa ttcggcagcc aggcgctgga cgcgcccggc tacgaactgc
     3301 tggcgctggt gcatggcagt gaaggcatgc tggcggtgac gaccgaggtg acggtgcggc
     3361 tgctgccgcg cccgcgcctg gcgcgctgca tcatggccag cttcgacgat gtgcggcgcg
     3421 ccggcgaggc ggtggcggcg gtgatcgccg ccggcatcat cccggccggg ctggaaatga
     3481 tggacaaacc catgacggcg gcggtggaag acttcgtgca cgccggctac gacctgcagg
     3541 cggccgccat cctgctgtgc gagagcgacg gcacgccgga agaggtggag gaagaaatcc
     3601 agcgcatgtc gcaggtgctg agcgcggccg gcgcgaccag gatcgccgtc agccagtcgg
     3661 aggccgagcg cctgaagttc tggagcgggc gcaagaacgc cttcccggcc agtgggcgca
     3721 tcagccccga ctacatgtgc atggactcca ccattccgcg caagcgcctg gccgacatcc
     3781 tgctggcgat cggccagatg gagcagaagt acggtctggg ctgctgcaac gtgttccacg
     3841 ccggcgacgg caacctgcac ccgctgatcc tgttcgacgc caacgacccc gaccagctgc
     3901 atcgggccga actgttcggt gccgacattc tggagaccag cgtcgccatg ggcggcacca
     3961 tcaccggcga gcacggcgtg ggcgtggaaa agctcaactc catgtgcgtg cagttttcag
     4021 cggcggagaa tgagcagatg ttcggcgtca agcgcgcctt cgatccggcc ggcctgctca
     4081 accccggcaa gctgatcccg acgctgaacc gctgcgccga gtacggcaag atgctggtgc
     4141 gcggcgggcg gctgccgttt ccggagctgg
//
LOCUS       DAOQTZ010000172         4059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_24823_length_4059_cov_13.824925, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000172 DAOQTZ010000000
VERSION     DAOQTZ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24823_length_4059_cov_13.824925"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKN51_13250"
     CDS             <1..237
                     /locus_tag="PKN51_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HNT86110.1"
                     /translation="RFEAAVANSKSLSERPDNPTLLKIYSLYKQATVGDNAEPKPGFT
                     DMVARAKWDAWKKLAGTSADEAMNQYIALIESLR"
     gene            complement(264..1340)
                     /locus_tag="PKN51_13255"
     CDS             complement(264..1340)
                     /locus_tag="PKN51_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT86111.1"
                     /translation="MVWLAAGLILAGLGGCTPAGYYAQAVGGHLALLQAARPIDELLA
                     DPASPAELKSRLALARRIRAFAVAELGLPDNASYQRYSDLRRRAAVWNVAAAPPDSLT
                     LRRWCFPVAGCVAYRGYYDEAEARALAERLAREEGLEVRVYGVPAYSTLGWLNWAGGD
                     PLLSTFIRYPEGELARLLFHELAHQQVYVGGDTAFNESYATAVERLGVARWLSSQAGD
                     AARREYAAFDARRQAFRQLVRQTRAELAAIYEQNRVQALTESALAAMKSEVMAGFRQR
                     YQALKASWVAAGTPFDAYDPWVAGANNAFFGIQAAYDDGVPAFEALFAREGGDWGRFH
                     AAVQALAALPQAERQQRLQALADG"
     gene            1483..2622
                     /locus_tag="PKN51_13260"
     CDS             1483..2622
                     /locus_tag="PKN51_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT86112.1"
                     /translation="MNDPLSFNRRRFHTVVGASLVACSRVVRAQDSRIVLGQSAAFSG
                     PAMQLGTQFHAGAKLCFDQLNARGGVNGRPIEIRHLDDGYEPDRCKANTERLIRDEVL
                     ALFGYIGTPTSLAALPLATAAKVPFIAPFTGAMGLREPFHREAFHLRASYDDETELIV
                     RQLTVLGQNRIAVFHQNDAYGQAGLSGVTKALTARQLKPLASATVERNSSDVREAVAR
                     LATSGAQAIVQIGAYQACAAFIREARKAGYGGTFHNVSFVGTQALADALGPDGAGVVV
                     SQVVPSPYDAARPITREFVAATRQSGGKVQANYSSMEGYLAAKVLAEGVRRAGAKLTR
                     DALVTGLESINEDFGGFRVAFGPGKHVASHFAELSMLTGDGRVRT"
     gene            complement(2732..>4059)
                     /gene="ffh"
                     /locus_tag="PKN51_13265"
     CDS             complement(2732..>4059)
                     /gene="ffh"
                     /locus_tag="PKN51_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNT86113.1"
                     /translation="RMALLEADVALPVVRDFIARVKDKALGQEVLGSLKPGQALVGIV
                     NRELAATMGEGVADLNLASQPPAVVLMAGLQGAGKTTTTAKLARHLIQKRKKKVLTVS
                     GDVYRPAAIEQLKTVTAQAGADWFPSSPEQQPVEIARAALDHAKKHFYDVLLVDTAGR
                     LAIDEALMQEIKALHAALNPVETLFVVDAMQGQDAANTARAFKEALPLTGIVLTKMDG
                     DARGGAALSVRQITGAPIKFAGVSEKIDGLEVFDAERHAGRILGMGDVVALVEQVTAS
                     VDLAAAQKLAEKVKKGDGFDLQDFLGQLQQMKQMGGLSSLMDKLPAQMAAKASDADLT
                     RAEKDIRRKEGIINSMTPAERRKPELLKASRKRRIAQGAGVQVQEVNRLLKEFEQMQG
                     MMKQFKGGALAKMMRRMGGMKGMQGLLGGGAMPPLPPGLGGPGGGKRPF"
BASE COUNT          605 a         1467 c         1362 g          625 t
ORIGIN      
        1 cgcttcgaag ccgccgtcgc caactccaag tcgctcagcg agcgccccga caacccgacc
       61 ctgctgaaga tctattcgct ctacaagcag gccaccgtcg gcgacaacgc cgagcccaag
      121 cccggcttca ccgacatggt ggcgcgcgcc aagtgggatg cctggaaaaa gctcgccggc
      181 acctcggccg acgaggccat gaaccagtac atcgcgctga tcgaatcgct gcgctgagcc
      241 gcagcaggcc gcgacggcgg tcctcagcca tccgccagcg cctgcagccg ctgctggcgc
      301 tcggcctgcg gcagcgccgc cagcgcctgc accgccgcgt gaaagcgccc ccagtcgccg
      361 ccttcgcggg caaacagtgc ctcgaaggcc ggcacgccgt cgtcgtaggc cgcctggatg
      421 ccgaagaacg cgttgttggc accggccacc caggggtcgt aggcgtcgaa cggcgtaccg
      481 gccgccaccc agctggcctt gagcgcctgg tagcgctggc gaaaacccgc cattacttca
      541 gatttcatag ctgccagcgc agactcagtg agcgcctgaa cccgattttg ttcataaatc
      601 gcggccagct cggcgcgcgt ctggcgcacc agttgcctga acgcctggcg gcgggcgtcg
      661 aacgccgcgt actcgcgccg ggccgcgtcg ccggcctggc tcgacagcca gcgcgccaca
      721 cccaggcgct cgaccgcggt ggcgtaggac tcgttgaacg cggtgtcgcc gccgacatag
      781 acctgctggt gcgccagttc gtgaaacaga aggcgcgcca gctcgccttc cgggtagcgg
      841 atgaaggtgg acagcagcgg atcaccgccg gcccagttca gccagcccag ggtcgaatag
      901 gcgggcacgc cgtagacccg cacctccagc ccttcttcgc gcgccaggcg ctcggccagc
      961 gcgcgcgcct cggcctcgtc gtaataaccc cgataggcca cgcagccggc gaccgggaag
     1021 caccagcgcc tgagcgtcag tgaatccggc ggcgccgccg ccacgttcca caccgcggcc
     1081 cggcgccgca gatcgctgta gcgctggtag ctggcgttgt ccggcagacc gagctcggcg
     1141 acggcaaagg cgcggatgcg gcgcgccagc gccaggcgcg acttcagctc ggccggcgaa
     1201 gccgggtcgg ccagcagctc gtcgatcggg cgcgccgcct gcagcagcgc caggtgacct
     1261 ccgacggcct gtgcgtagta cccggccggc gtgcagccac ccaaacccgc caggatcagc
     1321 ccggcggcaa gccagaccag gcgcctgcgc ggcggcactt caggcgcccc cggtggccgc
     1381 cacgccacgg tcgatcgcga accccatgca cttgaattgg aacttttgta cgatgtgcgc
     1441 catggtgcaa cacagggccc gcgccggacg ggcaaacagc tcatgaacga tccattgtct
     1501 ttcaaccgac gccgctttca cacggtggtg ggcgccagcc tggtggcgtg cagccgggtg
     1561 gtgcgcgcgc aggactcgcg catcgtgctc ggccagtcgg ccgcattcag cgggccggcc
     1621 atgcagctgg gcacgcagtt tcacgccggc gccaagctgt gcttcgatca gctcaatgcc
     1681 cgcggcggcg tgaacgggcg cccgatcgag atccgccacc tggacgacgg ctacgaaccc
     1741 gatcgctgca aggccaacac cgagcgcctg atccgcgacg aggtgctggc cctgttcggc
     1801 tacatcggca cgcccaccag cctggcggcg ctgccgctgg cgaccgccgc caaggtgccg
     1861 ttcatcgctc ccttcaccgg cgccatgggc ctgcgcgagc cctttcaccg cgaggccttc
     1921 cacctgcgcg cgtcctacga cgacgaaacc gagctgatcg tgcgccagct cacggtgctg
     1981 gggcagaacc gcatcgccgt gttccaccag aacgacgcct acggccaggc cgggctgtcg
     2041 ggcgtgacca aggcgctgac ggcgcgccag ttgaagccgc tggccagcgc cacggtggag
     2101 cgcaactcgt cggacgtgcg cgaggcggtg gccaggctgg ccacgtcggg cgcccaggcg
     2161 atcgtgcaga tcggcgccta ccaggcctgc gcggccttca tccgcgaggc tcgcaaggcc
     2221 ggctatggcg gcaccttcca caacgtgtcg ttcgtcggca cgcaggcgct ggccgacgcc
     2281 ctgggccccg acggcgccgg cgtggtggtg tcgcaggtgg tgccctcgcc gtatgacgcg
     2341 gcgcgcccga tcacgcggga gttcgtcgcc gccacgcgcc agtcgggcgg caaggtgcag
     2401 gccaactact cgtccatgga aggctatctg gccgccaagg tgctggccga gggcgtgcgg
     2461 cgcgccggcg ccaagctcac gcgcgacgcg ctggtcaccg gactggagtc gatcaacgag
     2521 gatttcgggg gcttcagggt ggccttcggt ccgggcaaac acgtggcctc gcactttgcc
     2581 gagctgtcca tgctgaccgg cgacgggcgc gtgcgcacct gaagcgcggc gcctccggcc
     2641 ctcggccgga tcgccattcg ctatcaaatc agtagcttct ggcgcttggc agacaagcgc
     2701 cggggccgaa ttggcctcag atcctggcga ctcagaacgg gcgcttgccg ccccccggcc
     2761 cgcccaggcc aggcggcagc ggcggcatgg cgccaccgcc cagcaggcct tgcatgccct
     2821 tcatgccgcc catgcggcgc atcatcttgg ctagggcgcc gcctttgaac tgcttcatca
     2881 tgccctgcat ctgctcgaac tccttgagca ggcggttgac ctcctgcacc tgcacgccgg
     2941 cgccttgggc aatgcggcgc ttgcggctgg ccttgagcag ttcgggcttg cggcgctcgg
     3001 caggcgtcat gctgttgatg atgccttcct tgcggcgaat gtctttttcg gcgcgcgtca
     3061 ggtcggcgtc gctggccttg gcggccatct gcgccggcag tttgtccatc aggctggaca
     3121 ggccgcccat ctgcttcatc tgctgcagct ggcccagaaa gtcctgcaga tcgaagccgt
     3181 cgcctttctt gaccttctcg gccagcttct gcgccgccgc caggtccacg ctggcggtga
     3241 cctgctcgac cagggccacc acgtcgccca tgcccaggat gcggccggcg tggcgctcgg
     3301 cgtcgaacac ctccaggccg tcgatctttt cggacacgcc ggcaaacttg atcggcgcgc
     3361 cggtgatctg ccgcaccgac agcgcggcgc cgccgcgcgc gtcgccgtcc atcttggtca
     3421 gcacgatgcc ggtgagcggc agcgcttcct tgaaggccct ggcggtgttg gccgcgtcct
     3481 ggccctgcat ggcatcgacc acgaacaggg tctcgacggg gttcagggcg gcgtgcaggg
     3541 ccttgatctc ctgcatcagt gcctcgtcga tcgccagccg gccggcagta tcgaccagca
     3601 gcacgtcgta gaagtgcttt ttggcgtggt ccagcgcggc gcgggcgatc tcgaccggct
     3661 gctgctcggg cgaggacggg aaccagtcgg cgccggcctg cgccgtcacg gttttcagct
     3721 gctcgatggc ggcgggacga tagacgtcgc ccgacaccgt cagcaccttc ttcttgcgct
     3781 tttgaatcag gtggcgcgcc agcttggcgg tggtggtggt cttgcccgcg ccctgcaggc
     3841 cggccatcag caccacggcg ggcggctggg aggccaggtt gaggtcggcc acgccctcgc
     3901 ccatggtcgc ggccagctcg cggttgacga tgcccaccag cgcctggccg ggtttcaggc
     3961 tgcccagcac ctcctggccc agcgccttgt ccttgacgcg ggcaatgaag tcgcgcacca
     4021 ccggcagggc cacgtcggct tcgagcaggg ccatgcgca
//
LOCUS       DAOQTZ010000173         4032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_25070_length_4032_cov_18.744782, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000173 DAOQTZ010000000
VERSION     DAOQTZ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25070_length_4032_cov_18.744782"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKN51_13270"
     CDS             complement(<1..349)
                     /locus_tag="PKN51_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HNT86114.1"
                     /translation="MYTQSFSVPDDEGKAAGPKTVERPASEREAALQAQFDARIDADG
                     RIEPRDWMPEAYRKTLVRQISQHAHSEIVGMLPEGNWISRAPSLKRKAILIAKVQDEG
                     GHGLYLYSAAETLG"
     gene            complement(690..1799)
                     /locus_tag="PKN51_13275"
     CDS             complement(690..1799)
                     /locus_tag="PKN51_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86115.1"
                     /translation="MYLRSISPKSPSSSSSMEASAAMGEVAAGAARLRMITSLMALVA
                     ASALSACGGGSESGAAPESATVASSATSESSDELESIQAKANRPKPVPSPSPAPAPAP
                     SSNAGRPIPMPMYGVTVDDVSKLKSITDSLSRLSRTPTTRIVFDEYQPASDYRKAAVA
                     INKVSYVMGEILDSQYLTTVNVQGYLDRTREYLATLGDVVDIWEIANEINGEWLGNNA
                     DVVAKMSGAYDIAKGQGRTTALTLYYNQDCWSKRSNEMFTWAQANIPERMKQGLDYVL
                     VSYYEEDCNNLKPDWNAVFQRLATMFPNSRLGFGEVGTSKAAQKAAYLQRYYAIKPPV
                     ANWAGGHFWWYFKQDMVPYTKPLWNTLNQAISANP"
     gene            complement(2320..3105)
                     /locus_tag="PKN51_13280"
     CDS             complement(2320..3105)
                     /locus_tag="PKN51_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86116.1"
                     /translation="MPLPLHGVTVDDVQDLPAITESLSRLSRTPTTRIVFDEYQPASD
                     YRKAAVAINKVSYVMGEILDSQYLTTVNVQGYLDRTREYLATLGDVVDIWEIANEING
                     EWLGNNADVVAKMSGAYDIAKGQGRTTALTLYYNQDCWSKRSNEMFTWAQANIPERMK
                     QGLDYVLVSYYEEDCNNLKPDWNAVFQRLATMFPNSRLGFGEVGTSKAAQKAAYLQRY
                     YAIKPPVANWAGGHFWWYFKQDMVPYTKPLWNTLNQAISANAP"
BASE COUNT          592 a         1225 c         1462 g          753 t
ORIGIN      
        1 tgcccagcgt ctcggcggcg gagtacaggt agaggccgtg gccgccctcg tcctgcacct
       61 tggcgatcag gatcgccttg cgcttgaggc tgggcgcgcg gctgatccag ttgccctcgg
      121 gcagcatgcc gacgatctcg gaatgcgcgt gctggctgat ctggcgcacc agcgtcttgc
      181 ggtaggcctc gggcatccag tcgcgcggct cgatgcggcc gtcggcgtcg atgcgggcgt
      241 cgaactgcgc ctgcagcgcc gcctcgcgct cgctggccgg tcgctcgacc gtcttcggcc
      301 cggcggcctt gccctcgtcg tcgggaacgg aaaaggattg ggtgtacacg tcgcggatct
      361 cctgggtgac cgacagcacc aagcccgcgc agcctcgaat gaccggacca cgcgggcgtg
      421 aagacccaaa gtatagtgag ttaaccgacc gaacggttgg ttaattgagg cgggatcgcc
      481 cagggtttac ccgggcgtcc gccgctgcgc gccccgcggg ccgggactgc gcagatgggc
      541 atgcatgccg agtgccgacg agagcggccc aaagcggccg atccgggccc cagacaccgc
      601 cgccgcaggg gctgcggcca ccatgggccg gcagggccgg tggtgggttg gggcggttct
      661 tgccggcact gcggaatcgt tggcgcccct caagggttgg cgctgatggc ctgattgagg
      721 gtattccaca ggggtttggt gtagggcacc atgtcctgct tgaagtacca ccagaagtgt
      781 ccgccggccc agttggccac ggggggtttg atggcgtagt agcgctgcag gtaggcggcc
      841 ttctgtgcgg ccttgctggt gcccacctcg ccaaagccca gacgcgagtt ggggaacatg
      901 gtggccaggc gctggaacac ggcgttccag tcgggcttga ggttgttgca gtcttcctcg
      961 tagtagctca ccagcacgta gtccagcccc tgcttcatgc gctcggggat gttggcctgc
     1021 gcccaggtga acatttcgtt gctgcgcttg gaccagcagt cctggttgta gtagagcgtc
     1081 agcgccgtgg tgcgaccctg gcccttggcg atgtcgtagg cgccgctcat cttggccacc
     1141 acatcggcgt tgttgcccag ccactcgccg ttgatctcgt tggcgatctc ccagatgtcg
     1201 accacatcgc ccagggtggc caggtactcg cgggtgcgat ccaggtagcc ctgcacgttg
     1261 acggtggtca ggtactgcga atccaggatc tcgcccatca cgtagctgac cttgttgatc
     1321 gccacggcgg ccttgcgata gtcgctggcc ggctggtact cgtcgaacac gatgcgcgtg
     1381 gtcggcgtgc gcgacagccg cgacagcgag tcggtgatgg acttcagctt gctgacatcg
     1441 tccacggtga cgccgtacat cggcatgggg atgggtcggc cggcgttgga tgaaggggct
     1501 ggggcgggcg ccggagaggg actgggaacc ggcttgggtc ggttggcctt ggcttgaata
     1561 ctttccagct catccgacga ttcgcttgtc gcgctggaag cgaccgtggc ggactccggc
     1621 gccgcgccgg attcgctgcc tccgccgcag gcgctgagcg ccgaggccgc caccagggcc
     1681 atcagcgagg tgatcatgcg cagcctggcg gcgccagcgg cgacctcgcc catggcggcc
     1741 gaggcctcca tcgaggatga agaagatggt gatttgggtg aaattgagcg tagatacatg
     1801 cggatgaatg tagattccat cccttcatca catgtaaagc ccggtacggt tacatctgcg
     1861 ccacagcgcg cccgggcggc ctcgttgcgg gtggccgaag ccgccggtga gtgggcggct
     1921 gcgccgtgag gaaccctgcg gtgcggtgat aggttttcat acaagtgtaa ttcttttaac
     1981 ttccgtcatg gcgtgaaatg acgcagaatt gatcgaaaag ttacaacaaa actgggtaga
     2041 ggttggtgcg acgcgacacg ccgtcagcgg ccgcgggtgc cgtggctctc cccgctgtgg
     2101 atcggtgggt cgatcgatgg tggcgaatca ggcgttggcg cacctcgccc cgtgcgggct
     2161 ggcgcagcgc cgtgccggat cggccgcggt ggcggtgtcg tcgacgtcgg cggatgggga
     2221 gtgccggcgt ggcttgccga acgggcaggg cggtcgcctc gatgacgtgc cggcgtgggg
     2281 caggcgggcg gggtgcctgt cagccttcgt cgcccgggtt cagggtgcgt tggcgctgat
     2341 ggcctgattg agggtgttcc acaggggttt ggtgtagggc accatgtcct gcttgaagta
     2401 ccaccagaag tgtccgccgg cccagttggc cacggggggt ttgatggcgt agtagcgctg
     2461 caggtaggcg gccttctgtg cggccttgct ggtgcccacc tcgccaaagc ccagacgcga
     2521 gttggggaac atggtggcca ggcgctggaa cacggcgttc cagtcgggct tgaggttgtt
     2581 gcagtcttcc tcgtagtagc tcaccagcac gtagtccagc ccctgcttca tgcgctcggg
     2641 gatgttggcc tgcgcccagg tgaacatttc gttgctgcgc ttggaccagc agtcctggtt
     2701 gtagtagagc gtcagcgccg tggtgcgacc ctggcccttg gcgatgtcgt aggcgccgct
     2761 catcttggcc accacatcgg cgttgttgcc cagccactcg ccgttgatct cgttggcgat
     2821 ctcccagatg tcgaccacat cgcccagggt ggccaggtac tcgcgggtgc gatccaggta
     2881 gccctgcacg ttgacggtgg tcaggtactg cgaatccagg atctcgccca tcacgtagct
     2941 gaccttgttg atcgccacgg cggccttgcg atagtcgctg gccggctggt actcgtcgaa
     3001 cacgatgcgc gtggtcggcg tgcgcgacag ccgcgacagc gattcggtga tggcgggcag
     3061 atcctggaca tcgtccaccg tcacgccgtg caggggcagc ggcacggtgc gccccccgcc
     3121 cgccggcggc gtcggtgcag gcgcaggcgc aggtgcaggc gcaggtgcag gtgcaggtgc
     3181 aggtgcaggt gcaggtgcag gtgcaggtgc gggtgcgggt gcgggtgcgg gtgcgggtgc
     3241 gggtgcaggc gaaggtgatg gactgggtgc tggagcgggc gtcaggcccg aggctgcttc
     3301 ggagccgccg ccgcaggcgc ccaccagacc ggctgacgct gcgatcagca gcgccaacgt
     3361 gcggcggctg gtggagcggc gcgctgggcc gggtgtccgg agaggtgctg gctgggagga
     3421 ggggggcggg cagctggccg cggagggggc gttctcggtt tccaatatcg gcatgcggcc
     3481 gcagtctagg caggccgatc cggcgtcggg taagggcttg tttcgcccca gtgcagtgtt
     3541 ttgcgctgtc aggcacttat ggaagccaga aaaaaggctt gcggcaaggt gcggctcgca
     3601 gcccatgctg gcggccttgg caacaggcgc aacgccgccg ccagcctttt tctggcttcc
     3661 cgcagggtga gccgccaagg gaccatctgc ttggtggcgt ggccttgaca tggctccatc
     3721 atgcctgcgg ccacgcgccg cgcagctggc cccttggcga ctcgctatag gtacctgaca
     3781 gcccgaaaca ctgcaccagc ccaccgcggt gccggtggat acatctcttg ccttgttcaa
     3841 accagaagcc tgaccctaga aacggccgtt gaccgcttgt caacttcggg cgggccgcat
     3901 cgatgcgggg tctggcggct gcgatggcct tcacctcctg ggtgagtccg ctgcgcaccc
     3961 acccgcaccg cgcgcgccgc gccatggccg cggcgccagc ggctcagtcg ccggtgtcca
     4021 cgctccagct gg
//
LOCUS       DAOQTZ010000174         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_26338_length_3889_cov_13.321075, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000174 DAOQTZ010000000
VERSION     DAOQTZ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26338_length_3889_cov_13.321075"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PKN51_13285"
     CDS             <1..675
                     /locus_tag="PKN51_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HNT86117.1"
                     /translation="SLRALIIAAVAAACSVAVAAPYAVNYQGNISSSDFPEITVGESY
                     TLTLVFDNGGTSANSQTWGAAHLTCAIWRMNNARNVVYAQNLVATPPSTVTGSATTNG
                     AGGLTGMFSSVSSIPGDPGYPAGSFTSSGISLTAPVHWFANSANGVFYDALGGTQNRE
                     FSDAAGGVIMNPANWSAPTPFSGSCSAAPSAVGVAPVPTLGEWGLGLLGLAAAGLGAQ
                     RLRRRR"
     gene            835..1884
                     /locus_tag="PKN51_13290"
     CDS             835..1884
                     /locus_tag="PKN51_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT86118.1"
                     /translation="MIQRLTLLATAALLCAGSAHAQDNVLNLYSARHYSTDEALYNDF
                     TKATGIKINRVDADDAGIIARLKAEGSASPADVILLVDAARLARGEQDGLFLPIKSKL
                     LEDTIPASLRAQPTADGATPWFGFSTRARVIVYDKMKVKPTEVDSYEKLADPQNKGKL
                     CIRSGSHPYNLSLFGAVLEHMGPQKTEAWLKGLVDNMARAPKGGDTDQIKAVAAGECQ
                     IAVTNSYYLARLMRSSKPEDRTVAERVGVVFPNQSSWGTHVNIAGAAVARHSKHVDSA
                     TRFLEYLASPSAQNYFANGNNEWPVAKGLVTDNPALKAMTGGSGQFKSETIPVGAVGA
                     NQVKVQQMLDRVGFK"
     gene            complement(1978..3126)
                     /locus_tag="PKN51_13295"
     CDS             complement(1978..3126)
                     /locus_tag="PKN51_13295"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNT86119.1"
                     /translation="MSVLPKPWARITLAGLLWSLASGAPAQNPPELQAQTVASGLQHP
                     WAVAFLPGGRFLVTERPGRLRVVDADGRVQPPLHGLPDIAAGGQGGLLDVITDAAFER
                     NRTIYFCFSQPGPGATNGTALARARLSDDLTGLQELRVIFSQQPKVASTLHFGCRIVE
                     RRVDGRPDGTLWLTLGERFSRRDDAQRLDNHLGKIVRVGKDGHVPADNPLVGRPGALP
                     EIWSHGHRNVQGAALAPDGALWTHEHGPMGGDEINRPLPGGNHGWPRVSFGLHYDGSP
                     VGDGASGGAGLVAPLHHWTPSIAPSGMVFLSSDRYGPAWRGNLFVGALKHRQLLRIEL
                     KDGQVVAQHPLLQALGQRIRDVRQGPDGWLYVLTDSPSGQLLRLVEKR"
     gene            3569..>3889
                     /locus_tag="PKN51_13300"
     CDS             3569..>3889
                     /locus_tag="PKN51_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT86120.1"
                     /translation="MKRPQLVLVGNGMAGVRTLEELLKLAPDLYDITVFGAEPHPNYN
                     RILLSPVLAGEQTLEQIVLNDWSWYEDNGITLHTGCTVTEVNRARRMVHATRADGETI
                     SVPYD"
BASE COUNT          660 a         1341 c         1240 g          648 t
ORIGIN      
        1 tctcttcgtg ccctgatcat tgcggctgtc gcggccgcgt gttcagtcgc agttgccgcg
       61 ccgtatgccg tgaactatca aggcaacatc agcagctccg actttccaga aatcacggtc
      121 ggtgaaagct atacgctgac gctggtgttc gacaacggcg gcacgtctgc caattcgcaa
      181 acttggggcg ccgcccacct cacatgcgcc atctggcgta tgaacaatgc gcgtaacgtg
      241 gtgtacgcac agaacttggt cgccacacca ccgtctacgg tcaccggcag cgcaacgacc
      301 aatggtgcag ggggactgac gggtatgttt tccagtgtca gctccatacc tggcgaccca
      361 ggctatccag cgggctcctt cacgtctagt ggaatttcat tgactgcacc ggttcactgg
      421 tttgccaaca gtgcgaatgg tgttttctat gacgctctcg gtggcactca gaaccgagaa
      481 ttcagtgatg ctgctggcgg ggttatcatg aacccggcaa actggagtgc gccaacgccg
      541 ttttccggca gctgcagtgc tgcaccttct gccgttggtg tagcacctgt acctacgctt
      601 ggtgaatggg gattgggtct tttgggcttg gccgctgcgg gattgggtgc gcaacgcctg
      661 cgccgccgcc gctgaatctc ccacgtcgca atctgaggcc gccttagggc ggcttttctt
      721 ttgccatagc caccgcccca ccgcggcccg accgccctcg taacgagaac aattctcgtt
      781 taatatatca actgctccct cttttttgtt catgtctgca agaaggacct ctcaatgatt
      841 caacgcctca ccctcctggc caccgccgcc cttctgtgcg cgggttccgc gcacgcccag
      901 gacaatgtcc tgaacctgta ctccgcccgc cactactcga ccgatgaggc gctgtacaac
      961 gacttcacca aggccaccgg catcaagatc aaccgcgtgg acgccgacga cgccggcatc
     1021 atcgcgcgcc tgaaggccga gggcagcgcc tcgccggccg acgtgatcct gctggtggac
     1081 gctgcccgcc tggcgcgcgg cgagcaggac ggcctgttcc tgccgatcaa gtccaagctg
     1141 ctggaagaca ccatccccgc cagcctgcgc gcccagccga ccgccgacgg cgccacgccc
     1201 tggttcggtt tttccacccg cgcccgcgtc atcgtgtacg acaagatgaa ggtcaaaccg
     1261 accgaggtgg acagctacga aaagctggcc gatccgcaga acaagggcaa gctgtgcatc
     1321 cgcagcggtt cgcaccccta caacctgagc ctgttcggtg ccgtgctgga acacatgggc
     1381 ccgcagaaga ccgaggcctg gctcaagggc ctggtcgaca acatggcgcg cgcgcccaag
     1441 ggcggcgaca ccgaccagat caaggccgtg gcggccggcg aatgccagat cgccgtgacc
     1501 aacagctact acctggcgcg cctgatgcgc tcgagcaagc ccgaggaccg cacggtggcc
     1561 gagcgcgtcg gcgtggtgtt ccccaaccag tcgagctggg gcacgcatgt caacatcgcc
     1621 ggcgcggcgg tggcgcgcca ttccaagcat gtggacagcg ccaccaggtt cctggaatac
     1681 ctggccagcc cgtcggcgca gaactacttt gccaacggca acaacgagtg gccggtggcc
     1741 aagggcctgg tcaccgacaa ccccgccctc aaggccatga ccggcggcag cggccagttc
     1801 aagagcgaga ccattccggt aggcgccgtg ggcgccaacc aggtcaaggt acagcagatg
     1861 ctggaccgcg tcggcttcaa gtgaagcggc gcttcgccct ggcttgccaa gcgccgccca
     1921 gcggcgcttt tttcacgctg agtcgagctg cagcgattgc ctggcaagcg ccaacagcta
     1981 tcgtttttca accaagcgga gcagctgccc ggacgggctg tcggtcagca cgtagagcca
     2041 gccgtcgggg ccctgcctga cgtcgcggat gcgctggccc agcgcctgca gcagcgggtg
     2101 ctgcgccacc acctggccgt ccttcagttc gatgcgcagc agctgccgat gcttgagcgc
     2161 gccgacgaac aggttgccgc gccaggccgg cccgtagcga tcgctgctca gaaacaccat
     2221 gcccgaaggc gcgatcgagg gcgtccagtg gtgcagcggg gccaccaggc cggcaccgcc
     2281 gctggcgcca tcgcccaccg gcgagccgtc gtagtgcaag ccaaagctca cccgcggcca
     2341 gccgtggttg ccgcctggta gcgggcggtt gatttcgtcg cctcccatcg ggccatgctc
     2401 gtgcgtccac agcgcgccgt cgggtgccag cgcggcgccc tggacgttgc ggtggccatg
     2461 gctccagatc tcgggcaagg cgcccgggcg gccgaccagc gggttgtccg cgggcacgtg
     2521 gccgtccttg cccacacgca cgatcttgcc caggtggttg tccagccgct gggcgtcgtc
     2581 gcggcgcgag aagcgctcgc ccagcgtcag ccacagcgtg ccgtcgggcc gcccatcgac
     2641 ccgccgctcg acgatgcggc agccgaaatg cagtgtgctg gcgaccttgg gttgttggct
     2701 gaagatcaca cgcagctcct gcaggcccgt caggtcgtcg ctcagccgcg cacgcgccag
     2761 cgccgtgccg ttggtcgcgc ccggcccggg ctgggagaag cagaaataga tcgtgcggtt
     2821 gcgctcgaac gccgcatcgg tgatcacgtc cagcaggccg ccctgccctc cggcggcgat
     2881 gtcgggcagg ccatgcagcg gtggctgcac ccggccgtcg gcgtcgacca cgcgcaggcg
     2941 ccccggccgc tcggtcacca gaaatcgtcc tccaggcaga aaggccacgg cccaagggtg
     3001 ctgcaagccg ctggccacgg tctgcgcctg cagctcgggt gggttttgcg ccggcgcccc
     3061 cgatgccagc gaccacagca ggccggccag cgtgatccgg gcccagggct ttggcaagac
     3121 gctcatcgtg acgctccatt cgcccgtgcg ggcgtgccgt gccaagtgtc accgcaccgc
     3181 cccggccggg gcctccagcg tagcgcagag cgggacttgt ggcttgctcg gtcacccgct
     3241 ggcggcccgg gccgatcgag cctgagcgct aagtcccggc ggacgcacca cgaaggctgt
     3301 cagaactggt gcgccctgcg cgtcgcgctg caccattttg tggcgcaggc cccaggacga
     3361 tgcagcggcc ctttgtgtcc cagggggcag accgtcgggt cgcagctggt ctggccccat
     3421 ggccacgccg ctgcgcgggg aacctcgatc tggcgcggat cttgcttgaa gcaggtcagg
     3481 cctgcatttc cgggggccgt gccacacgcc tcggcgttcg gtcggcaccc gggaagcagt
     3541 ccctgacctt gtcacaggat ttgctgcaat gaagagaccc caactcgtgc tggtaggcaa
     3601 tggcatggcg ggcgtgcgca cgctggagga gctgctcaag ctcgcgcccg atctctatga
     3661 catcacggtg ttcggcgccg agccacaccc caactacaac cgcatcctgc tctcgcccgt
     3721 gctggccggc gagcaaaccc tggagcagat cgtgctcaac gactggtcct ggtatgagga
     3781 caacggcatc accctgcaca ccggctgcac ggtgacggaa gtcaaccgcg cccggcgcat
     3841 ggtgcatgcg acgagggccg atggcgagac catcagcgtg ccctacgac
//
LOCUS       DAOQTZ010000175         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_26656_length_3856_cov_13.936859, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000175 DAOQTZ010000000
VERSION     DAOQTZ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26656_length_3856_cov_13.936859"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..319
                     /gene="soxZ"
                     /locus_tag="PKN51_13305"
     CDS             8..319
                     /gene="soxZ"
                     /locus_tag="PKN51_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HNT86121.1"
                     /translation="MADPMRIRAQASGDKVTVRVLMSHEMESGQRKDASGKLVPAWHI
                     QDVTASLNGKPVFSCEWGPAVSKNPFLQFAIKGAKAGDKVSVTWKDNKGDSRTDEATV
                     S"
     gene            407..1216
                     /gene="soxA"
                     /locus_tag="PKN51_13310"
     CDS             407..1216
                     /gene="soxA"
                     /locus_tag="PKN51_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HNT86122.1"
                     /translation="MRGKPIATVVAALGLIASAGGALAQKTSIQAIEEYREMLQDGNP
                     ADLFEAKGEDLWKKKRGPKNASLERCDLGKGPGVVKGAFVELPRWFADTGKVQDLESR
                     LITCMSTLQGIDPKEIINGQWGKGERANTTALATWIAAESKGMAFNLPQSHAQERIMY
                     QVGKRLFYQRGGAHDFACASCHGEEGKRIRLQDLPDLRKAPGDGIGFAAWPAYRVSNG
                     QMWSMQHRLNDCYRQQRFPEPTFASDVTVALGVFLGVNAKGDKSAAPTIKR"
     gene            1233..1904
                     /gene="soxX"
                     /locus_tag="PKN51_13315"
     CDS             1233..1904
                     /gene="soxX"
                     /locus_tag="PKN51_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HNT86123.1"
                     /translation="MTHRTSILALSIAGALTLTGCAGGGSAGVPSAAELDTLTQQIVQ
                     RDFHDKGIVKVSQAFTDDETMRVCNAADVSGKPLDDATAKRIEALNMATIKWPANGKY
                     LGDWKEGEKLAQSGRGLTWTDKADTPNGGNCYNCHQIDKKEISFGTIGTSLYNYGKLR
                     GVTDPNSPQAQEMVKYTWGKIWNSKAYNACSNMPRAGHKGILTEKQVADLVALLLDPN
                     SPVNK"
     gene            1966..3702
                     /gene="soxB"
                     /locus_tag="PKN51_13320"
     CDS             1966..3702
                     /gene="soxB"
                     /locus_tag="PKN51_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HNT86124.1"
                     /translation="MNLSKREFLQVLSAASVAGMGLGRFAEASAQTAAEGLYDIPRFG
                     NVSFLHITDCHAQLRPIYFREPSVNLGVGDMKGRLPHLVGEHLLKAAGVPAGGAGAHA
                     LTYLDFEQAAQRYGKVGGFAHLATLVKRVRASRPGSLLVDGGDTWQGSGTALWTNGQD
                     MVDACKLLGVDAMTGHFEFTLGMKRVQEIVEKDFAGKVDFVAQNIKTADFGDPVFKPY
                     VIRQMNGVPVAIVGQAFPYTPIANPRWMVAEWEFGIQDENMQKVVDEARAKGAQVVVV
                     VSHNGMDVDLKMASRVRGIDAILGGHTHDGMPVASIVKNAGGQTIVTNAGSNGKFLGV
                     LDFDVKGGKVADFRYKLLPVFANMLPADKDMDALISKIRAPYEAKLSEQLAITEGLLY
                     RRGNFNGTGDQLIVDALMEVQGADLAFSPGFRWGTSLLPGQPITREWVMDMTATTYSY
                     ATVTEMKGEMIKTVMEDVADNLFNPDPYYQQGGDMVRVGGLSYSFDPLAPMGKRVSDM
                     RLKGQLIDADKSYKVAGWAPVAEDAAKAGNPMVWDLVEKWLKARGGKVAPRQLNNPKL
                     VGGVLPNPGYAA"
     gene            3718..>3856
                     /locus_tag="PKN51_13325"
     CDS             3718..>3856
                     /locus_tag="PKN51_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HNT86125.1"
                     /translation="MSDLGRFIEAQADRCAAALAELRAGAKRTHWIWFILPQLRGLGT
                     SP"
BASE COUNT          751 a         1349 c         1227 g          529 t
ORIGIN      
        1 aacagacatg gcagatccaa tgcgaatccg cgcgcaggcg tccggcgaca aggtcacggt
       61 gcgcgtgctc atgagccacg aaatggaatc cggtcagcgc aaggacgcgt ccggcaagct
      121 ggtgcccgca tggcacattc aggacgtcac cgcctcgctc aacggcaagc cggtgttttc
      181 gtgcgaatgg ggcccggcgg tgtccaagaa cccgttcctg cagttcgcca tcaagggcgc
      241 caaggcgggc gacaaggtca gcgtgacctg gaaggacaac aagggcgaca gccgcaccga
      301 cgaggccacg gtgtcctgat cgcccggtga tcgcccccac ggcgggcacg gcccttgaag
      361 gacctgcccg ccgttttctt ttcatccgaa acaggagaca agcacgatga gaggcaaacc
      421 catcgccacg gtggtcgcag cactgggcct gatcgcgtcg gccggcggcg cgctggcgca
      481 gaagacgtcg atccaggcca tcgaggaata ccgcgagatg ctgcaggacg gcaacccggc
      541 cgacctgttc gaagccaagg gcgaggacct ttggaagaaa aagcgcggcc ccaagaacgc
      601 gtcgctggaa cgctgtgacc tgggcaaggg cccgggcgtg gtcaagggtg ccttcgtcga
      661 gctgccgcgc tggtttgccg acaccggcaa ggtgcaggac ctcgaatcgc gtctgatcac
      721 ctgcatgagc acgctgcagg gcatcgaccc caaggaaatc atcaacggcc agtggggcaa
      781 gggcgaacgc gccaacacca ccgccctggc cacctggatc gccgccgaat ccaagggcat
      841 ggccttcaac ctgccgcagt cgcatgcgca ggaacgcatc atgtaccagg tgggcaagcg
      901 cctgttctac cagcgcggtg gcgcgcacga cttcgcctgc gcctcctgcc acggcgaaga
      961 aggcaagcgc attcgcctgc aggacctgcc cgatctgcgc aaggcgccgg gcgacggcat
     1021 cggctttgcc gcctggccgg cctaccgcgt ctccaacggc cagatgtggt ccatgcagca
     1081 ccgcctgaac gactgctacc gtcagcagcg cttccccgaa ccgacgttcg ccagcgacgt
     1141 caccgtggcc ctgggcgtgt tcctgggcgt caacgccaag ggcgacaaat ccgccgcccc
     1201 aaccatcaag cgctgaaccg gaggcccgca ccatgaccca ccgcacatcc atcctcgccc
     1261 tgtccatcgc cggcgccctg accctgaccg gctgcgccgg cggcggctcc gccggcgtcc
     1321 cctcggcggc cgagctcgac accctgacgc agcagatcgt ccagcgcgac ttccacgaca
     1381 agggcatcgt caaggtcagc caggccttca ccgacgacga aaccatgcgc gtgtgcaacg
     1441 ccgccgacgt gtccggcaag ccgctcgacg acgccaccgc caagcgcatc gaggcgctca
     1501 acatggccac catcaagtgg cccgccaacg gcaagtacct gggcgactgg aaagaaggcg
     1561 aaaaactggc gcaaagcggc cgcggcctga cctggaccga caaggccgac acccccaacg
     1621 gcggcaactg ctacaactgc caccagatcg acaagaagga aatctccttc ggcaccatcg
     1681 gcaccagcct gtacaactac ggcaagctgc gtggcgtgac cgaccccaac agcccgcagg
     1741 cgcaggaaat ggtcaagtac acctggggca agatctggaa cagcaaggcc tacaacgcct
     1801 gctccaacat gccgcgcgcc ggccacaagg gcatcctgac cgaaaagcag gtggccgacc
     1861 tggtggcgct gctgctcgac cccaactcgc cggtcaacaa gtgaccggcc ccagcccggc
     1921 gctgccgggc ttttttgctt cctttaattc agtggttgct gatatatgaa cctctccaaa
     1981 cgcgaattcc tgcaggtgct cagcgccgcc agcgtggccg gcatggggct gggccggttt
     2041 gccgaagcca gcgcgcagac cgccgccgaa gggctgtacg acatccccag gttcggcaac
     2101 gtgtcgttcc tgcacatcac cgactgccac gcgcagctgc ggcccatcta cttccgtgaa
     2161 cccagcgtca acctgggcgt gggcgacatg aagggccgcc tgccgcacct ggtgggcgag
     2221 cacctgctca aggccgccgg cgtgccggcc ggcggtgccg gcgcgcacgc gctgacctac
     2281 ctcgacttcg agcaggccgc ccagcgctac ggcaaggtgg gcggctttgc ccacctggcc
     2341 accctggtca agcgcgtgcg cgccagccgc ccgggctccc ttttggtcga cggcggcgac
     2401 acctggcagg gcagcggcac cgcgctgtgg accaatggcc aggacatggt ggacgcctgc
     2461 aagctgctgg gcgtggacgc catgaccggc cacttcgagt tcaccctggg catgaagcgc
     2521 gtgcaggaaa tcgtcgagaa ggactttgcc ggcaaggtcg acttcgtggc gcagaacatc
     2581 aagaccgccg acttcggcga cccggtgttc aaaccctacg tgatccgcca gatgaacggc
     2641 gtgccggtgg ccatcgtcgg tcaggccttc ccctacaccc ccatcgccaa cccgcgctgg
     2701 atggtggccg aatgggaatt cggcatccag gacgagaaca tgcagaaggt ggtcgacgaa
     2761 gcccgcgcca agggtgccca ggtggtggtc gtcgtcagcc acaacggcat ggacgtggac
     2821 ctgaagatgg ccagccgcgt gcgcggcatc gacgccatcc tgggcggcca cacgcacgac
     2881 ggcatgccgg tggccagcat cgtcaagaac gccggcggcc agaccatcgt caccaacgcc
     2941 ggcagcaacg gcaagttcct cggcgtgctg gacttcgacg tcaagggcgg caaggtggcc
     3001 gacttccgct acaagctgct gccggtgttt gccaacatgc tgccggccga caaggacatg
     3061 gacgcgctca tcagcaagat acgcgccccc tacgaggcca agctgtccga gcaactggcc
     3121 atcaccgaag gcctgctgta ccggcgcggc aacttcaacg gcaccggcga ccagctcatc
     3181 gtcgatgcgc tgatggaagt gcagggcgcc gacctggcgt tctcgcccgg cttccgctgg
     3241 ggcaccagcc tgctgcccgg ccagcccatc acgcgcgaat gggtgatgga catgaccgcc
     3301 accacctaca gctacgccac cgtcaccgag atgaagggcg agatgatcaa gaccgtgatg
     3361 gaagacgtgg ccgacaacct gttcaacccc gacccctact accagcaggg cggcgacatg
     3421 gtgcgcgtcg gtggcctgag ctacagcttc gatcccctgg cgcccatggg caagcgcgtg
     3481 agcgacatgc gcctgaaggg ccagctgatc gacgccgaca agagctacaa ggtggccggc
     3541 tgggcgccgg tggccgaaga cgccgccaag gccggcaacc ccatggtctg ggacctggtc
     3601 gaaaaatggc tcaaggcgcg cggtggcaag gtggcgccgc gccagctcaa caaccccaaa
     3661 ctggtgggcg gcgtgctgcc caacccgggc tacgcggcct gacctgccgg cgccgccgtg
     3721 tccgacctcg gccgcttcat cgaggcgcag gcggaccgct gcgccgcggc gctggccgag
     3781 ctgcgcgccg gcgccaagcg cacgcactgg atctggttca tcctgccgca gctgcggggc
     3841 ctgggcacca gcccgc
//
LOCUS       DAOQTZ010000176         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_26657_length_3856_cov_13.122599, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000176 DAOQTZ010000000
VERSION     DAOQTZ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26657_length_3856_cov_13.122599"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKN51_13330"
     CDS             <1..555
                     /locus_tag="PKN51_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HNT86126.1"
                     /translation="LEDYHVGPFHPGLGNFVTCDDLKWQFGREYSVQTVGVAPSFGKP
                     GSDVYRKWHEVLLKYREGRLPERGAIWLTYYPHIMVEWYPHVLTVSTLHPTGVDRTLN
                     MVEFYYPEEIACFEREFVEAQRAAYMETAIEDDEIAERMDAGRKALMLRGDNEVGPYQ
                     SPMEDGMQHFHEWYRRAMGMRVTT"
     gene            676..1563
                     /locus_tag="PKN51_13335"
     CDS             676..1563
                     /locus_tag="PKN51_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT86127.1"
                     /translation="MQAAWMVAASLFFALMSVCIKFASPYFGPLEIVCYRGAIGVLFM
                     WALCRSRGVRLHTSVPMMHVWRNVVGVSALVCWFYAIAHLPLATAMTLNYMSGVWVAA
                     FLVGGTLLMGQLADIRRQGPLVMAVMAGFAGVVMMLRPTLEQNQLFAGLIGLLSGLMS
                     ALAYIQVAALGRVGEPEARTVFYFSLGAALAGGIGMQFFDTHPLHTVQALWLLPVGIL
                     AALGQLCMTRAYTRGATMVVANLQYSGIVFAALFGLLLFGDQLPLIGWLGMATIVASG
                     IAATVLRARALPNPPAESH"
     gene            1578..2438
                     /locus_tag="PKN51_13340"
     CDS             1578..2438
                     /locus_tag="PKN51_13340"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNT86128.1"
                     /translation="MTYTHLITVAELQALQASGAPLMVFDCSGELGNPARADAMFAEQ
                     HIAGSRQAHLERDLSAHPPAEAINGGRHPLPPRERFAAWLSAVGFANPMQAVVYDRNG
                     CNYCGRLWWMFKWAGHDAVAVLDGGLQAWLAAGGAVASGPAAEHPATRFQLQPPRRRL
                     LHVHEVQAGLGQPGQTVIDARAAARFRGDEEPLDPVAGHIPGALNRPYVDNLDGRGCF
                     KAPAQLRAEFDALLAGRDPAGVVHHCGSGVSAVPNLLAMELAGYAPAALYAGSWSEWC
                     RTPGLPVARG"
     gene            complement(2777..3688)
                     /locus_tag="PKN51_13345"
     CDS             complement(2777..3688)
                     /locus_tag="PKN51_13345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNT86129.1"
                     /translation="MLKSDHLNDAQALLNLNDLPAAPSRRRALRLGVGMGYAAAALPV
                     MAQTAIRTPTDGLTVGEVNIDVAGFSMPAFRAQPAGRSGLPVVLVLSEIFGVHEYIAD
                     TAVRLARAGYLAIAPELFVRQGDAQSYGEMAKLMSEVIAKVPDAQVMGDLDATVAWAA
                     QHGGNSDKLAVTGFCWGGRQTWLFAAHHPKVKAGVAWYGRLVGTATPLTPRHPVDVAA
                     QLKAPVLGLYGGADTGIPLDTVEQMKAALAAAGTQGNAAARGSAFVVYPDAPHAFHAD
                     YRPSFRKEAAEDGWKRALAWFKQHGVA"
BASE COUNT          574 a         1345 c         1306 g          631 t
ORIGIN      
        1 ctggaggact accacgtcgg ccccttccac cccggcctgg gcaacttcgt cacctgtgac
       61 gacctgaagt ggcagttcgg gcgcgagtac tcggtgcaga cggtgggcgt ggcgcccagc
      121 ttcggcaaac cgggctcgga cgtctaccgc aagtggcacg aggtgctgct gaagtaccgc
      181 gaaggccggc tgcccgagcg cggcgcgatc tggctgacct actacccgca catcatggtc
      241 gagtggtatc cgcatgtgct caccgtctcc accctgcacc cgacgggcgt ggacaggacg
      301 ctgaacatgg tggagttcta ttaccccgaa gagatcgcct gcttcgagcg cgagttcgtc
      361 gaggcccagc gcgccgccta catggaaacc gcgatcgagg acgacgagat cgccgagcgc
      421 atggacgccg gccgcaaggc cctgatgctg cgcggcgaca acgaagtcgg cccctaccag
      481 agcccgatgg aagacggcat gcagcacttc cacgagtggt accggcgtgc catgggcatg
      541 cgcgtcacca cctgagcgcg cggcaccctg agcccgcggc gcctggcggc aaacgcccca
      601 ggcgctattt tattgatagc tgctggcgca catgggacaa gcgctgcagg catttttgtc
      661 tgataatcac acgccatgca agccgcctgg atggtcgccg cctcgctctt cttcgccctg
      721 atgagcgtgt gcatcaagtt cgcctcaccc tacttcgggc cgctggagat cgtgtgctat
      781 cgcggcgcca tcggcgtgct gttcatgtgg gcgctgtgcc gctcgcgcgg cgtgcgcctg
      841 cacaccagcg tgcccatgat gcacgtgtgg cgcaacgtgg tcggcgtgtc ggcgctggtg
      901 tgctggttct acgccatcgc ccacctgccg ctggccaccg ccatgacgct gaactacatg
      961 agcggcgtct gggtggcggc cttcctggtc ggcggcacgt tgctgatggg gcagctggcc
     1021 gacatccgcc gccagggccc gctggtgatg gcggtgatgg ccggctttgc cggcgtggtg
     1081 atgatgctgc gccccacgct ggagcagaac cagctgtttg ccgggctgat cgggctgctg
     1141 tcgggcctga tgtcggcgct ggcctacatc caggtggcgg cgctcggccg cgtcggcgag
     1201 ccggaggcgc gcacggtgtt ctatttctcg ctcggcgcgg cgctggcggg cggcatcggc
     1261 atgcagttct tcgacaccca tccgctccat accgtgcagg cgctgtggct gctgccggtt
     1321 ggcatcctgg cggcgctggg gcagctgtgc atgacacgcg cctacacccg cggcgccacc
     1381 atggtggtgg ccaacctgca atactcgggc atcgtgttcg cggccctgtt cggcctgctg
     1441 ttgttcggcg atcagctgcc gctgatcggc tggctgggca tggccaccat cgtcgccagc
     1501 ggcatcgcgg ccaccgtgct gcgcgcccga gccctgccca acccgccggc tgaaagtcac
     1561 tgaacccagg ctgacccatg acctataccc acctcattac cgtcgccgaa cttcaggcgc
     1621 tgcaggccag cggcgcgccg ctgatggtgt tcgactgcag cggcgaactg ggcaacccgg
     1681 cccgggccga cgccatgttt gccgaacagc acatcgccgg cagccgtcag gcgcatctgg
     1741 agcgcgacct cagcgcccac ccgccggccg aggccatcaa cggcgggcgc cacccgctgc
     1801 ccccgcgtga gcgctttgcc gcctggctgt ccgcggtcgg ctttgccaac cccatgcagg
     1861 cggtggtgta cgaccgcaac ggctgcaact actgcggccg cctgtggtgg atgttcaagt
     1921 gggcgggtca cgacgcggtg gcggtgctgg acggcggcct gcaggcctgg ctggcggcgg
     1981 gcggcgcggt ggcctccggc ccggcggccg agcaccccgc cacccggttc cagctgcagc
     2041 caccgcggcg ccgcctgctg cacgttcacg aggtgcaggc cgggctgggc cagcccgggc
     2101 agaccgtgat cgacgcccgc gccgccgcgc gttttcgcgg tgacgaggag ccgctcgatc
     2161 cggtggccgg ccacatcccc ggcgcgctca accgccccta cgtcgacaac ctggatgggc
     2221 gcggctgctt caaggcgccg gcccagctgc gcgccgaatt cgacgccctg ctggccggcc
     2281 gcgacccggc cggcgtggtg caccactgcg gcagcggcgt cagcgccgtg cccaacctgc
     2341 tggccatgga gctggccggc tatgccccgg cggcgctgta cgccggcagc tggagcgagt
     2401 ggtgccgcac cccagggctg ccggtggcac gcggctgaag cccgatggct cttgtggcgt
     2461 tgcgtgctgg atggaacaaa caaaaccggt ggattgctgg ccaggatgag gcgcaaacca
     2521 cagcagcagc tccgctatgg cgcggatttg caacgagaca tcatgggcga aaagacacgg
     2581 tttgatgttc catatggcgc gaaactctgc actatattat caatagcgtc tggcgcttgc
     2641 ctgacgcgcc tcgcaagcct tttccgttct gcccggcgcc gatccaccgc acaccctgct
     2701 gccagccgac caggcccacg atgcgtgcgc ctgcggtggc gcgcaaggca gggcgccggc
     2761 gatcgcctcg ggcacctcag gccacgccgt gctgcttgaa ccaggccagc gcgcgcttcc
     2821 agccgtcctc ggccgcttcc ttgcgaaagc tcgggcggta gtcggcatgg aaggcgtgcg
     2881 gcgcatccgg gtagaccacg aacgccgaac ccctggccgc agcgttgccc tgcgtgccgg
     2941 cggccgccag cgcggccttc atctgctcga ccgtgtccag cgggatgccg gtgtcggcgc
     3001 cgccatacag gcccagcacc ggcgccttca gctgcgccgc cacatccacc gggtggcggg
     3061 gcgtcagcgg cgtggcggtg ccgaccaggc gcccatacca ggccacgccg gccttgacct
     3121 tggggtgatg ggcggcaaac agccaggtct ggcgcccgcc ccagcagaaa ccggtgacgg
     3181 ccagtttgtc ggagttgccg ccatgctgcg cggcccaggc cacggtggcg tccagatcgc
     3241 ccatcacctg agcatcgggc accttggcaa tcacctcgct catcagcttg gccatctcgc
     3301 catagctttg cgcgtcgccc tggcgcacga acaactcggg cgcgatcgcc aggtaaccgg
     3361 cacgcgccag acgcacggcg gtgtcggcga tgtactcgtg cacgccgaag atttccgaca
     3421 gcaccagcac caccggcagc ccgctcctgc cggccggctg ggcgcgaaag gccggcatgc
     3481 tgaagccggc cacgtcgatg ttcacctcgc ccacggtcag gccgtcggtg ggggtgcgga
     3541 tggcggtctg cgccatcacc ggcagggcgg ccgcggcgta gcccatgcca acgcccagcc
     3601 tcagcgcgcg ccggcgcgac ggcgccgccg gcagatcgtt caggttcagc agcgcctggg
     3661 cgtcgttcag gtgatcggat ttcagcattc tgtgtctccg cttgatggtg gcagcgccag
     3721 tctagagcgg atcgacgcag gatgggagga tctcaccccg aaggccagcc acgcgcgcat
     3781 accgtatgtc ctataatcaa cggtactcag tggtggctgt agctcagttg gtagagtcca
     3841 ggattgtgat tcctgt
//
LOCUS       DAOQTZ010000177         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_26938_length_3828_cov_11.527432, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000177 DAOQTZ010000000
VERSION     DAOQTZ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26938_length_3828_cov_11.527432"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKN51_13350"
     CDS             <1..1057
                     /locus_tag="PKN51_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNT86130.1"
                     /translation="DMAAYRASLERFVYQTGMVMRPVYAAAKAVAPQAKRVAYAEGED
                     ERVLRAAQVVLDDGIATPILIGRPAVIEQRIAKAGLRMRLGQDVECVNPEDDRRFRQY
                     WEAYHGLMSRDGVTVDAAKAAVRRSTTIIAALMVHLGDADAMLCGTLGRFDQHLEHVR
                     DIIGVEPGAPGYATLNALMLPDRTLFVTDTYVNEDPSAELLTAIAKMAADEVSNFGLP
                     PRLAFVSHSNFGSSHRPSALKVRRARDLFRDRHPGIMCDGEMHGDSALSATLRERAVA
                     DSTLQGEANLLVCPNLDAANILFNVLKITIGSGVTIGPILLGTAAPAHVLTTSSTVRR
                     VINMTALAVAEAQARRR"
     gene            1450..>3828
                     /locus_tag="PKN51_13355"
     CDS             1450..>3828
                     /locus_tag="PKN51_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNT86131.1"
                     /translation="MSIRNLDSLFDPQSVAVIGASERPFSVGGTLWRNMHDGGFAGPV
                     YPVNPKHRTLSGQRCYASVDDLPEAPELAVICTPAHTVVELIGQLAARGTRAAVVISA
                     GLTAEQKQAMLDAARPSLLRVLGPNCVGLLAPHARLNASFAHIPARPGELAFVSQSGA
                     LVTAMLDWAEARQIGFSHFVSLGEHADVDFGDLLDYLASDARTRAILLYIESIDQARK
                     FMSAARAAARNKPVIVIKAGRSAQGQKAAASHTGALAGADDVVDAAIARAGMLRVDSL
                     EELFLAAEILTRQTAPIGDRMTILTNGGGVGVLAADAAAAHDVPLAELSPALVGALDA
                     VLPANWSRGNPVDIIGDAPAQRYVDALQALTRHPQEVGTVLFIQAPTAIVPSTDIARA
                     LLPALRPEGGAPLPLVSCWVGGPAVAEARQLFIEAGIASYDMPEQAVAAIAMLQAYAR
                     NQAELAEAPPAQLLHNGSVPDAARVRALVQGVLASGRDMLTEPEAKAVCEAYHIPVVA
                     TRTTPADPEAAAAEAARIGFPVVIKILSEDISHKSDVGGVVLNLQDEADVRAAASAML
                     ARIRKSQPKARLDGFSVQAMVRRKQAQELIIGASIDPSFGPVILFGQGGTAVEVMKDS
                     AMALPPLNDPLARALIARTRVARLLAGYRDVPAADLDAVVATLMAVSQLLADVPEVAE
                     LDINPLIVNHEGAIALDARVRVSPRLPAGAAHFAIAPYPAQLIETLEWKGQRLTLRPI
                     RPEDELQHRAFLESLDAEDVRMRLFYSRRTMERSELARLVQIDYTREMAFIAT"
BASE COUNT          570 a         1411 c         1320 g          527 t
ORIGIN      
        1 cgacatggcg gcctaccgcg ccagcctgga gcgcttcgtc taccagaccg gcatggtgat
       61 gcgcccggtc tacgccgccg ccaaggcggt ggcgccgcag gccaagcgcg tggcctacgc
      121 cgaaggcgag gacgagcgcg tgctgcgcgc cgcccaggtg gtgctggacg acggcatcgc
      181 cacgccgatc ctgatcggcc gcccggcggt gatcgagcag cgcatcgcca aggccggcct
      241 gcgcatgcgc ctgggccagg acgtggaatg cgtcaacccc gaagacgacc ggcgcttccg
      301 tcagtactgg gaggcctacc acggcctgat gtcgcgcgac ggcgtcaccg tggacgctgc
      361 caaggcggcg gtgcgccgct ccaccaccat catcgcggcg ctgatggtgc acctgggcga
      421 cgccgacgcc atgctgtgcg gcacgctggg gcgcttcgac cagcacctcg agcacgtgcg
      481 cgacatcatc ggcgtcgaac ccggcgcccc gggctacgcc acgctgaacg cgctgatgct
      541 gcccgaccgc acgctgttcg tcaccgacac ctacgtcaac gaagatccca gcgccgagct
      601 gctgacggcg atcgccaaga tggcggccga tgaagtcagc aacttcggcc tgccgccccg
      661 gctggccttc gtgtcgcact ccaacttcgg ctcctcgcac cgtccgtcgg cgctgaaggt
      721 gcgacgcgcg cgcgacctgt tccgcgaccg gcacccgggc atcatgtgcg acggcgagat
      781 gcacggcgac agcgccctgt cggccacgct gcgcgagcgc gccgtcgccg acagcacgct
      841 gcagggcgag gccaacctgc tggtctgccc caacctggac gccgccaaca tcctgttcaa
      901 cgtgctcaag atcaccatcg gcagcggcgt caccatcggc cccatcctgc tgggcacggc
      961 ggcgccggcg cacgtgctga ccacctcatc caccgtgcgg cgggtcatca acatgacggc
     1021 gctggcggtg gccgaggcgc aggcgcgccg acgctgacct ggggcggtgg ggcgtgcgct
     1081 ccaccaccga tgaccgatgg cgagtggccc atgaggcggg ctgcgccctg catgaccgat
     1141 gacccatctg tcaaccaagc gcagcgaggt cattggccat gggtcattct cgaattgcta
     1201 tttttaatgt agctgccggc gcttgccaga taagcgctga cggccttttt tgctctgaca
     1261 tggcgttcgc cgcggcggcg cgtttggacg gcaggtcatg gtccggtggc tgcgtggtgc
     1321 aggggcagca agcccggccc ggaccacggg gtaggatcaa gggttgtgca cacggtgcgc
     1381 cctggccccg gccatcggcc gccttcccac gcacgcgccc ggccgtgcgg ttccgagttc
     1441 tgacaagtca tgagcatccg caacctcgat tccctgttcg accctcagtc ggtggccgtc
     1501 atcggcgcct ccgagcgacc cttttccgtc ggcggcacgc tgtggcgcaa catgcacgac
     1561 ggcggctttg ccggcccggt ctatccggtc aaccccaagc accgcacgct gtcgggccag
     1621 cgctgctacg ccagcgtgga cgatctgccc gaagcgcccg agctggcggt gatctgcacc
     1681 cccgcccaca cggtggtcga gctgatcggc cagctggccg cgcgtggcac gcgggcggcg
     1741 gtggtcatca gcgccggcct cacggccgag cagaagcagg ccatgctgga cgccgcccgc
     1801 cccagcctgc tgcgcgtgct gggccccaac tgcgtcggcc tgctggcgcc gcacgcgcgg
     1861 ctgaacgcca gcttcgccca catcccggcg cggccgggcg agctggcctt cgtctcgcag
     1921 tcgggcgccc tggtcaccgc catgctggac tgggccgaag cgcgacagat cggcttttcg
     1981 cacttcgtgt cgctgggcga acacgccgac gtcgacttcg gtgacctgct ggactacctg
     2041 gccagcgacg cccgcacacg cgccatcctg ctgtacatcg agtcgatcga ccaggcacgc
     2101 aagttcatgt cggcggcgcg cgcggcggcg cgcaacaagc cggtcatcgt catcaaggcc
     2161 gggcgcagcg cgcagggcca gaaggccgcg gcctcgcaca ccggcgcgct ggccggcgcc
     2221 gacgacgtgg tggacgcggc gattgcccgc gccggcatgc tgcgggtgga cagtctggaa
     2281 gagctgttcc tggcggccga gatcctgacg cgccagaccg ccccgatcgg cgatcgcatg
     2341 accatcctga ccaacggcgg cggggtgggc gtgctggcgg ccgacgccgc cgccgcccac
     2401 gacgtgccac tggcggaact gagcccggcc ctggtcggcg cactggacgc ggtgttgccg
     2461 gccaactggt cgcgcggcaa cccggtggac atcatcggcg acgcgccggc gcagcgctac
     2521 gtcgacgcgc tgcaggcact gacgcgccac ccgcaggaag tcggcacggt gctgttcatc
     2581 caggcgccca ccgccatcgt gccctcgacc gacatcgcgc gtgccctgct gccggcgctg
     2641 cggccggaag gtggcgcgcc gctgccgctg gtcagctgct gggtcggcgg cccggcggtg
     2701 gccgaagcgc gccagctgtt catcgaagcc ggcatcgcca gctacgacat gcccgagcag
     2761 gccgtggccg ccatcgccat gctgcaggcc tacgcccgca accaggccga gctggccgag
     2821 gcgccgccgg cccagctgct gcacaacggc agcgtgcccg acgcggcgcg cgtgcgcgcc
     2881 ctggtgcagg gcgtgctggc cagcggccgc gacatgctga ccgaacccga ggccaaggcg
     2941 gtgtgcgagg cctaccacat cccggtggtg gccacgcgca ccacgccggc cgaccccgag
     3001 gcggcggccg ccgaagcggc gcgcatcggc tttccggtgg tcatcaagat cctgtcggaa
     3061 gacatctcgc acaaatccga cgtcggcggc gtggtgctca acctgcagga cgaagccgac
     3121 gtgcgcgccg ccgccagcgc catgctggcg cgcatccgca agagccagcc gaaggcgcgg
     3181 ctggacggct tcagcgtgca ggccatggtg cggcgcaagc aggcgcagga gctgatcatc
     3241 ggcgccagca tcgaccccag cttcggcccg gtgatcctgt tcggccaggg cggcacggcg
     3301 gtcgaggtga tgaaggacag cgccatggcg ctgccgccac tgaacgaccc gctggcgcgc
     3361 gcgctgatcg cgcgcacccg cgtggcgcgc ctgctggccg gctaccgcga cgtgccggcg
     3421 gccgacctgg acgccgtggt cgccaccctg atggcggtgt cgcagctgct ggccgacgtg
     3481 cccgaggtgg ccgagctcga catcaaccca ctgatcgtca accacgaagg cgccattgcg
     3541 ctggatgcgc gcgtgcgcgt cagcccgcgc ctgccggccg gcgcggcgca ctttgccatc
     3601 gcgccctacc cggcgcagct gatcgagacc ctggaatgga aaggccagcg cctgacgctg
     3661 cgcccgatcc gccccgaaga cgaactgcag caccgcgcct tcctggaaag cctggacgcc
     3721 gaggacgtcc gcatgcgcct gttctacagc cgccgcacca tggagcgcag cgagctggcg
     3781 cggctggtgc agatcgacta cacgcgcgag atggccttca tcgccacc
//
LOCUS       DAOQTZ010000178         3827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_26952_length_3827_cov_13.534464, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000178 DAOQTZ010000000
VERSION     DAOQTZ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26952_length_3827_cov_13.534464"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKN51_13360"
     CDS             complement(<1..434)
                     /locus_tag="PKN51_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT86132.1"
                     /translation="MQEKFDPQAVERAAHAHWQATDAYRVTEDAAKPKYYACSMLPYP
                     SGKLHMGHVRNYTINDMLTRQLRMKGYNVLMPMGWDAFGLPAENAALKNGVPPARWTY
                     DNIAHMKGQMQAMGLAIDWSREIATCDPEYYRWNQWLFLKML"
     gene            complement(558..1007)
                     /locus_tag="PKN51_13365"
     CDS             complement(558..1007)
                     /locus_tag="PKN51_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNT86133.1"
                     /translation="MAFGRLERTKGSEPMSDINVTPLVDVMLVLVVIFIITAPLLASS
                     IRLDLPKTDGAKPGDPPKFVTVVVDKSGQAFFEDKPVSQAQLSVALQQARLDNPDTEV
                     QLRADQGVPYGRVVEVMGEAQKAGLNRIGFVAESPAAGEAPQPAASR"
     gene            complement(1151..1783)
                     /locus_tag="PKN51_13370"
     CDS             complement(1151..1783)
                     /locus_tag="PKN51_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT86134.1"
                     /translation="MNLQSVFSQSDAIGKAVAILLLLMSIASWVVILWKAWMLRRAGG
                     DVVRGVAAFWQAPTLDEAASRVGGFDRGQLVLPLIQATQLPAGDTLAAAGDRAQQLTR
                     TLRDALHAVMGRLQFGQVLLATIGSTAPFVGLLGTVWGIYHALTGIAGSGQISIERVA
                     GPVGEALIMTAAGLAVAIPAVLAYNWFGRVIGRIEAELEGFARDLREMLK"
     gene            complement(1805..2620)
                     /gene="dapB"
                     /locus_tag="PKN51_13375"
     CDS             complement(1805..2620)
                     /gene="dapB"
                     /locus_tag="PKN51_13375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNT86135.1"
                     /translation="MTDTSTSLRVAIAGATGRMGQMLIDAVRAAGDCTLSGALERADS
                     PALGQDAGAHAGWASGVTITADLDAGLRGAAVLIDFTRPEATLAHLAVCQRLGVAMVI
                     GTTGFTDAQKAAIAAAARDIAIVMAPNMSVGVNVTLKLLEMAARALSTGYDIEVIEAH
                     HRHKVDAPSGTALRMGEVIAAALGRDLNDCAVYERVGHTGERDPSSIGFATIRGGDIV
                     GDHTVLFAGTGERIEISHKSSSRAGYAQGSLRAARFLAGRASGLYDMADVLGL"
     gene            complement(2675..3235)
                     /locus_tag="PKN51_13380"
     CDS             complement(2675..3235)
                     /locus_tag="PKN51_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNT86136.1"
                     /translation="MPVSSFFHVRAIARVMPVVLAAALGACSSFDQGTRNLADALTLY
                     RPEVVQGNFVSREQVGALQPGMTRLQVRDILGTPLITSLFHEQRWDYVFTMQRQGVAP
                     QRYRLTVFFNGDVLERFDGDDMPSETEFVQRIGRERKLKVPVLEASEEQLAKFPTSSP
                     GGADGAADAAETAQPAASYPPLEPAR"
     gene            3311..3760
                     /gene="fur"
                     /locus_tag="PKN51_13385"
     CDS             3311..3760
                     /gene="fur"
                     /locus_tag="PKN51_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNT86137.1"
                     /translation="MDNIDELKSTGLKATLPRLKILEIFQKAEQRHMTAEDVYRHLLA
                     EQSDIGLATVYRVLTQFEQAGLLSRNHFEVGKAVYELREEQHHDHLVCLDCGHVEEFH
                     DPEIEKRQTAVAKARGFTLVDHALSLYAHCTKANCPHRGSGTPATRR"
BASE COUNT          602 a         1291 c         1354 g          580 t
ORIGIN      
        1 tccagcatct tcaggaacag ccactggttc cagcggtagt actcggggtc gcaggtggcg
       61 atctcgcggc tccagtcgat ggccaggccc atggcctgca tctggccttt catgtgggcg
      121 atgttgtcgt aggtccagcg cgccggcggc acgccgttct tcagtgccgc gttctcggcc
      181 ggcaggccga aggcgtccca gcccatgggc atgagcacgt tgtagccctt catgcgcagc
      241 tggcgcgtga gcatgtcgtt gatggtgtag ttgcgcacat ggcccatgtg cagcttgccg
      301 ctggggtagg gcagcatgga gcaggcgtag tacttgggct tggccgcgtc ctcggtgacg
      361 cggtaggcgt cggtggcctg ccagtgggcg tgggcggcgc gctcgacggc ctgagggtcg
      421 aatttttctt gcatgggagg cgattgtagg tgcgcgtgcg gccgaccgcg gcacgcacgg
      481 cgcttccgtt tactatgatt tatatagctg cttgcgcacg ctgggcgggc gcaaaagccc
      541 aaaatgatgc agaaacatca gcgcgaggcg gccggctggg gcgcctcgcc cgcagccggc
      601 gattcggcca cgaagccgat gcggttcagg cccgccttct gcgcttcgcc catcacctcg
      661 accacgcggc cgtagggcac gccctggtcg gcgcgcagct gcacctcggt gtcggggttg
      721 tccaggcgcg cctgctgcag cgccaccgac agctgggcct gggacaccgg cttgtcctcg
      781 aagaaggcct ggccgctctt gtccaccacc acggtgacga acttgggcgg atcgcccggc
      841 ttggcgccgt cggtcttggg caggtccagc cggatcgagc tggccagcag cggcgcggtg
      901 atgatgaaaa tcaccaccag caccagcatc acgtccacca gcggcgtgac gttgatgtcg
      961 ctcatgggct cgctgccctt ggtgcgttcg agtctgccga atgccatggg tgaatctcag
     1021 gcgtgcgcgc agcagcacgc agcgaagggc cgcagggcag gccaggccag aaacgccgtg
     1081 gccggacctg cgccggccac aggcgtcgtt ccccctgtca ggggggaagg cgcgaagcga
     1141 ctcagggcgg tcatttcagc atttcgcgca ggtcgcgcgc aaagccttcc agctcggcct
     1201 cgatgcggcc gatgacgcgg ccgaaccagt tgtaggccag caccgccggg atcgccaccg
     1261 ccaggccggc ggcggtcatg atcagggcct cgcccaccgg gccggccacg cgctcgatgc
     1321 tgatctgccc cgaaccggcg atgccggtca gggcgtgata gatgccccac accgtgccca
     1381 gcaggccgac gaagggcgcg gtcgagccga tggtggccag cagcacctgg ccgaactgca
     1441 ggcgccccat gacggcgtgc agcgcgtcgc gcagcgtgcg cgtgagttgc tgcgcgcggt
     1501 cgcccgcggc cgccagcgtg tcgccggccg gcagctgcgt ggcctggatc agcggcagca
     1561 ccagctggcc gcggtcgaag ccgccgacgc ggctggccgc ttcgtccagc gtcggcgcct
     1621 gccagaaggc ggccacgccg cgcaccacgt cgccgccggc gcgccgcagc atccaggcct
     1681 tccacaggat caccacccag ctggcgatgg acatcagcag cagcaggatg gccacggcct
     1741 tgccgatggc gtcgctctgg gaaaaaacgg attgcaggtt catggttcgc gaaaaatgcg
     1801 acggttacag gcccagtacg tcggccatgt cgtacaggcc gctggcccgg ccggccagaa
     1861 agcgcgccgc gcgcaggctg ccctgggcgt agccggcgcg gctgctggac ttgtggctga
     1921 tctcgatgcg ctcgccggtg ccggcgaaca gcaccgtgtg atcgcccacg atgtcgccac
     1981 cgcggatggt ggcaaagccg atgctggaag ggtcgcgctc gccggtgtgg ccgacgcgct
     2041 cgtacaccgc gcagtcgttg aggtcgcgcc ccagcgcggc ggcgatcacc tcgcccatgc
     2101 gcagcgccgt gccgctgggg gcgtcgacct tgtggcggtg atgcgcctcg atcacctcga
     2161 tgtcgtagcc ggtggacagc gcgcgggcgg ccatctccag cagcttgagg gtgacgttga
     2221 cgcccacgct catgttgggc gccatgacga tggcgatgtc gcgcgcggca gcggcgatgg
     2281 cggccttctg cgcatcggtg aaaccggtgg tgccgatcac catcgccacg cccaggcgct
     2341 ggcagacggc caggtgcgcc agggtggctt cggggcgcgt gaagtcgatc agcaccgcgg
     2401 cgccgcgcag gccggcgtcc agatcggcgg tgatggtcac gcccgaggcc cagccggcat
     2461 gggcgccggc atcctggccc agcgccgggc tgtcggcgcg ctcgagtgca cccgacagcg
     2521 tgcagtcgcc cgcggcgcgc acggcatcga tcagcatctg gcccatgcgg ccggtggcgc
     2581 cggcaatggc cacgcgcagc gaggtggagg tgtcggtcat gggcgaatca ggacgtgaag
     2641 atgcggccag ccgaggccgc gccgccggtg cgcctcagcg cgccggctcc aggggtgggt
     2701 agctggcggc cggctgggcc gtctcggcgg cgtcggcggc gccatcggcc ccgccggggg
     2761 aactggtcgg gaacttggcg agctgctctt cgctggcctc gagcaccggc accttgagct
     2821 tgcgttcgcg gccgatgcgc tgcacgaatt cggtttcgct gggcatgtcg tcaccgtcga
     2881 agcgctccag cacgtcgccg ttgaagaaca ccgtcaggcg gtagcgctgc ggcgccacgc
     2941 cctggcgctg catggtgaac acgtagtccc agcgctgctc gtggaacagg ctggtgatca
     3001 gcggcgtgcc caggatgtcg cgcacctgca ggcgcgtcat gccgggctgc agcgcgccca
     3061 cctgctcgcg cgacacgaaa ttgccctgca ccacctcggg ccggtacagc gtcagggcgt
     3121 cggccaggtt gcgcgtgccc tggtcgaagc tgctgcaggc gcccagcgcc gccgccagca
     3181 cgaccggcat gacccgcgcg atcgcgcgga catgaaaaaa ggaggaaaca ggcatgcgtg
     3241 tccgctcatg cacaatacgg ccggtcattg tagcggcggt cccgggcggg ccggccgcac
     3301 cctttcggcc atggacaaca tcgacgaact caagagcacc ggcctgaagg ccacgctgcc
     3361 gcgcctgaag atcctggaaa ttttccagaa ggccgagcaa cgccacatga cggcggaaga
     3421 cgtctatcgc cacctgctgg ccgagcagtc ggacatcggg ctggccaccg tctaccgggt
     3481 gctgacgcag ttcgagcagg ccggcctgct gagccgcaac catttcgagg tcggcaaggc
     3541 ggtctacgag ctgcgcgaag agcagcacca cgaccacctg gtgtgcctgg actgcggcca
     3601 cgtggaggaa ttccacgacc ccgagatcga gaagcgccag acggcggtgg ccaaggcgcg
     3661 cggcttcacc ctcgtcgacc atgcgctgag cctgtacgcg cactgcacca aggccaactg
     3721 cccgcaccgc ggcagcggca cgccggccac gcgccgctga atacctggtc aaacaggctt
     3781 ccaggcagct cgcagcaagc gccaacagct atttatttga tagtaat
//
LOCUS       DAOQTZ010000179         3794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_27261_length_3794_cov_16.365873, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000179 DAOQTZ010000000
VERSION     DAOQTZ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27261_length_3794_cov_16.365873"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PKN51_13390"
     CDS             <1..1191
                     /locus_tag="PKN51_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT86138.1"
                     /translation="MRHVLGLGVDAPGVTDWRLATAAAPDHPPAIEIGPDHLMRIAYT
                     SGTTGRPKGVAYTLERWQARLANHFLATEYGLSVDDAMLHVGPLTHAAGVYLFPCFLR
                     GARNVVLDQFDPATLLATIERERITHLMLVPTMMHRLVEAIEGGARGDWSSLRRIHYG
                     TAPTPVPLIRRAQAIFGPILRQQYGMTEAVQPLCVLYPHEHQGTSADGADEPIGSCGR
                     PTANVRIVLRDTQGREVAPGEVGEITIAHEGIGKVQYWRRPELLAETVRDGWYYSGDL
                     GRLDRQGFLHIVGRNKDMIISGGFNVYAREVEDALSTHPAVLEAAVLGLPDPQWGELV
                     AAAVVLRADCTASAEALMAHCGERIAGYKKPRRIAFVEALPRNLAGKVQKPALRERFT
                     SQGL"
     gene            complement(1816..2550)
                     /locus_tag="PKN51_13395"
     CDS             complement(1816..2550)
                     /locus_tag="PKN51_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT86139.1"
                     /translation="MESDQEVFARWERLIQFRLLADSQEPTRTVPEQVAAQVGDRIIG
                     GALAPGARIGEQELADEFAISRGPVREAIRILEREGLVQVLPRRGAVVTSLSAQELRE
                     LFEIRAGLVEIALRKVMGAGVPPELLAITRAGVARLEALAELPEGGDAYAETTYRLIQ
                     IGAHFAGNERLRRMVVALSLQSLRYSKLGLASVARRQRSVKLWRQALQALERGDVERT
                     LWLARQRSQESADEAARRLAEPSGGS"
     gene            complement(2695..3423)
                     /gene="lolD"
                     /locus_tag="PKN51_13400"
     CDS             complement(2695..3423)
                     /gene="lolD"
                     /locus_tag="PKN51_13400"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HNT86140.1"
                     /translation="MSEVAIENIAAGADPASAGIVLEAESLGRRFHEGPLDVTVLQGV
                     DLRVRAGETLAIVGQSGSGKSTLLHLLGGLDAPSSGRVRLLGQDLGSLSPAAQGELRN
                     RHLGFVYQFHHLLPEFSALENVAMPLWIRRQPRAEAAEAARHMLARVGLQERVRHRPS
                     ELSGGERQRVAIARALVTRPACVLADEPTGNLDRATADAVFALMMELAAQHGTAFIVV
                     THDAQLAGRCARVLRLVQGRLGPV"
     gene            complement(3416..>3794)
                     /locus_tag="PKN51_13405"
     CDS             complement(3416..>3794)
                     /locus_tag="PKN51_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT86141.1"
                     /translation="TLVMTVQDKRADIAILRTLGASPASIMKVFMVQGALVGVIGTLG
                     GLALGLAIAFHIDVIVPFLERLFHASFLPKDVYLISQMPSDPQAGDIVPIGVISLLLS
                     FLATLYPSWRASRVNPAEALRYE"
BASE COUNT          600 a         1354 c         1297 g          543 t
ORIGIN      
        1 atgcgccacg tgctcggtct cggtgtggac gcccccggag tgaccgactg gcggctggcc
       61 accgcggcgg caccggatca cccgccggcc atcgagatcg gacccgacca cctgatgcgc
      121 atcgcctaca ccagtggtac caccggtcgg cccaagggtg tggcctacac gctggagcgc
      181 tggcaggcgc gcctggccaa ccactttctg gccacggaat acggtctgtc ggtggacgac
      241 gccatgctgc acgtcggacc gctgacccac gccgccgggg tgtacctgtt tccctgtttt
      301 ctgcgcggtg cgcgcaacgt ggtgctggat cagttcgacc ccgccacgct gctggccacc
      361 atcgagcgcg agcgcatcac gcacctgatg ctggtgccga ccatgatgca ccggctggtg
      421 gaggccatcg aaggcggcgc gcgcggtgac tggtcctcgc tgcggcgcat tcactacggc
      481 acggcgccca ccccggtgcc gctgatccgg cgcgcccagg cgatcttcgg cccgatcctg
      541 cgccagcagt acggcatgac cgaggcggtg cagccgctgt gcgtgctcta tccgcatgag
      601 caccagggca ccagcgccga cggcgcggac gaaccgatcg gctcctgcgg gcgacccacg
      661 gccaacgtgc gcatcgtgct cagggacacc caggggcgcg aggtcgcgcc cggcgaggtg
      721 ggcgaaatca ccattgccca cgaaggcatc ggcaaggtgc agtactggcg ccgtcccgag
      781 ctgctggccg agaccgtgcg cgatggctgg tactacagcg gcgacctcgg gcggttggac
      841 cgacagggct ttctgcacat cgtcggccgc aacaaggaca tgatcatcag cggcggattc
      901 aacgtctacg cacgcgaggt ggaggatgcc ctgagcaccc accccgccgt gctggaggcc
      961 gccgtgctgg gcctgcccga cccgcagtgg ggcgagctgg tcgcggccgc ggtggtgctg
     1021 cgtgccgact gcaccgccag cgccgaggcg ctgatggccc actgcggcga gcgcatcgcc
     1081 ggctacaaga aaccgcggcg catcgccttc gtcgaggcgc tgccgcgcaa cctggccggc
     1141 aaggtgcaga agcccgcctt gcgcgaacgc ttcaccagcc agggcctgta aacgctaggc
     1201 aaggggtcgc cctgcatagc gttaacctag aagactgtcg gacttggaaa tcgccggctg
     1261 caaatcgacc gcagcggccc atttttcacc ggattttttg cccaagagcc cggctattgg
     1321 gctgcaaatc cggcaaaaac tggtctcgct ggagccgatt ttggctacgc cgctcttcga
     1381 gaacggtttc ggcgctccaa gcccgacagc ctcccagaaa cggccgttgc acgcgcccgc
     1441 ccgtgccgtg atcggtgtgc caggcaagac gcgacgccgc tgcgcgctcc tgcccgcaag
     1501 caggagcaac gccgcatggc gcggcgagcg cggtgcgggt gggtgcgtag cgtgctcacc
     1561 caggaggtga aggccatcgc agccgccaga ccccgcatcg atgcggtctg cccgaagttg
     1621 accagcggtc aactgccgtt tctaggttga aatgggcggt gagggacacc cgagacggga
     1681 ggcgatgcaa ggcgcaaagc gcagccacag cccgtgctgt ggcgagcatg ggcaacgccg
     1741 cagcgtgccc gtatcgggcg gacagcgccg atccgatttc agtgattcag tggactagaa
     1801 caggccggct gaagctcagc tgccgcccga cggctcggcc agccggcgcg cggcttcatc
     1861 ggccgactcc tgactgcgct ggcgcgccag ccacagggtc cgttccacgt cgccgcgctc
     1921 cagcgcctgc agcgcctggc gccacagctt caccgagcgc tgacgccgcg ccaccgaggc
     1981 caggcccagc ttgctgtagc gcaggctctg cagcgacagc gccaccacca tgcggcgcag
     2041 gcgctcgttg ccggcgaaat gcgctccgat ctggatcaac cggtaggtcg tctcggcata
     2101 ggcatcgccg ccctccggca gttcggccag cgcctcgagc cgggccacgc cggcgcgcgt
     2161 gatggccagc agctcgggcg gcacgcccgc gcccatcacc ttgcgcaacg cgatctcgac
     2221 caggccggca cggatctcga acagctcgcg cagctcctgt gccgacaggc tggtcaccac
     2281 cgcgccgcgg cgcggcagca cctgcaccaa tccttcgcgc tccaggatgc ggatggcttc
     2341 gcgcaccggc ccgcggctga tggcgaattc gtcggccagt tcctgctcgc cgatgcgcgc
     2401 gcccggcgcc agcgcgccac cgatgatgcg gtcgcccacc tgcgcggcca cctgctcggg
     2461 cacggtgcgg gtgggctcct ggctgtcggc cagcagccgg aactgaatca ggcgctccca
     2521 gcgcgcgaac acttcctggt cagactccac gctcgccatc gggtacgcct tgtttcatga
     2581 ttgtcgacaa tcataaatcc ggcccaggcg gtggcgcgca tgggtggcgc cggcaggctg
     2641 ccgggtcggc cgctccctcg gccaccaggc cacggccgcc caagggggca caggtcacac
     2701 cggcccgagc cggccctgca ccagccgcag caccctggcg cagcgccccg ccagctgggc
     2761 gtcgtgcgtg accacgatga aggccgtgcc gtgctgggcc gccagctcca tcatcagcgc
     2821 aaacaccgcg tcggcggtgg cgcggtcgag gttgccggtg ggctcgtcgg ccagcacgca
     2881 ggccgggcgg gtgaccaggg cgcgcgcgat ggccacgcgc tggcgctcgc cgcccgacag
     2941 ctcggacgga cggtgccgca cgcgctcctg cagacccacg cgcgccagca tgtggcgtgc
     3001 cgcctcggcc gcctcggcgc gcggctggcg gcggatccac agcggcatgg cgacgttttc
     3061 gagcgcgctg aattcgggca gcaggtggtg aaactggtag acgaagccca ggtggcggtt
     3121 gcgcagctcg ccctgcgccg ccggcgacag gctgccgagg tcctgcccca gcagccgcac
     3181 gcgcccgctg ctgggggcgt ccagcccgcc cagcaggtgc agcagggtgc tcttgccgga
     3241 gccggactgg cccacgatgg ccagcgtttc accggcgcgc acgcgcagat cgacgccctg
     3301 cagcaccgtc acgtcgagcg ggccctcatg aaagcgccgg cccaggcttt cagcctccaa
     3361 aacgatgcca gcgcttgctg gatctgcgcc agcagctatg ttttcaatag ctacttcact
     3421 catagcgcag cgcctcggcg gggttgacgc ggctggcgcg ccagctcggg tagagcgtgg
     3481 ccaggaacga cagcagcagc gagatgacgc cgatcggcac gatgtcgccg gcctgcgggt
     3541 cgctgggcat ctggctgatc aagtacacgt ccttgggcag gaagctggcg tggaacaggc
     3601 gttcgaggaa cggcacgatg acgtcgatgt ggaaggcgat cgccaggccc agcgccagcc
     3661 cgcccagggt gccgatcacg cccaccagcg cgccctgcac catgaacacc ttcatgatgc
     3721 tggccgggct ggcgcccagg gtgcgcagga tggcgatgtc ggcgcgcttg tcctgcaccg
     3781 tcatcaccag cgtg
//
LOCUS       DAOQTZ010000180         3788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_27343_length_3788_cov_15.389767, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000180 DAOQTZ010000000
VERSION     DAOQTZ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27343_length_3788_cov_15.389767"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKN51_13410"
     CDS             complement(<1..376)
                     /locus_tag="PKN51_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNT86142.1"
                     /translation="MFKKILVANRGEIALRIQRACRELGVKAVMVYSEADKDAKYVRL
                     ADEAVCIGPAPSSQSYLHMPALISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFQF
                     IGPTPESIRIMGDKVSAKQAMIK"
     gene            complement(612..1082)
                     /gene="accB"
                     /locus_tag="PKN51_13415"
     CDS             complement(612..1082)
                     /gene="accB"
                     /locus_tag="PKN51_13415"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNT86143.1"
                     /translation="MDLRKLKTLIDLVSESNVSELEITEAEGKVRIVKSGQPAAAQVV
                     YAPVAAPAVAPAAPPAAAAPAAAAPQAVAEAAESTGFVVKSPMVGTFYRAASPGAKPF
                     VEVGQAVKEGDTLCIIEAMKILNEIEAEKSGTVTRIMGENGQAVEFGQPLFVIE"
     gene            complement(1209..1781)
                     /locus_tag="PKN51_13420"
     CDS             complement(1209..1781)
                     /locus_tag="PKN51_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT86144.1"
                     /translation="MTLPEPQPAPVVAPPARPLGRRLLVAGVALAAAGAGAWYAARRL
                     QLSEPQLAGFWERSFPTPDGSTLALAGLRGRPLLVNFWATWCPPCIAELPLLSAFYTE
                     NKANGWQVLGLAVDKPEPVGRFLAQTPVSFPVALAGLEGASLTRELGNSAGGLPFSVL
                     FDAQGTLRERKLGQLREGDLRAWRKAAGSA"
     gene            complement(1778..2374)
                     /locus_tag="PKN51_13425"
     CDS             complement(1778..2374)
                     /locus_tag="PKN51_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86145.1"
                     /translation="METLTQELAAHAARLVVEEGLDYGGAKRRAHKLMGLPARTALPD
                     NELVEAEVRAYLALFHADTQPAELAALRRLALRWMERLAEFRPHVTGAVWNGTATRRS
                     DVWLQLFCDDPKSAEISLIDHQVRYQARSINGLHGVPVDTLSFSSPCPELGEPIGVHL
                     MIHDHDELRGALKPGRLGQPLRGDRQALVQRMQMEPTP"
     gene            2446..>3788
                     /locus_tag="PKN51_13430"
     CDS             2446..>3788
                     /locus_tag="PKN51_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT86146.1"
                     /translation="MQATMMSTPLSLNHLLERAGRLFHDNTIVSRLPDKSLRRHSYGE
                     YYRRTRALAAALQGLGLQKGDRVATLCWNHHAHLECYFGIPAAGGVMHTLNLRLPPEE
                     IGWIAGDAADRFLIIDDVLLPLYQQFAHLHRFERVIVFAFSGAPVAPEFTDYEALLAA
                     ADPDAFDYAPHAEDDPTSMCYTSGTTGKPKGVVYSHRSTVLHSLVGALPDATAVRNTD
                     VVLPVTPMFHANSWGMPYIAVMLGAKLVFPGPHLHPEDLLDLMQQEPPTLTLGVPTIW
                     LAMIQLYDSSLQPGSPHHGRWQLPTGLRSMVGGAAVPESLIRAFGRHGVWIMQGWGMT
                     ETSPVGTLAYPRAELQGASDDERLRRAAMAGVPMPLVDLRIRGDHGQDQPWDGTSVGE
                     LQVRGPFITGGYHEAGAPPDKFTADGWLRTGDVASVDALGYMRISDRTKDLIKSGG"
BASE COUNT          553 a         1365 c         1255 g          615 t
ORIGIN      
        1 ccttgatcat ggcctgcttg gccgacacct tgtcgcccat gatgcggatc gactcgggcg
       61 tcggtccgat gaactgaaag ccgctctttt ccacccgttc ggcaaagtcg gcgttctcgc
      121 tcagaaagcc gtagccgggg tggatcgcct cggcgtcggt gacttcggcg gccgaaatca
      181 gcgccggcat gtgcaggtag ctctgcgacg acggcgccgg gccgatgcag acggcctcgt
      241 cggccagacg cacgtacttg gcgtccttgt cggcctcgga atagaccatc acggccttga
      301 cgcccagctc gcggcaggcg cgctggatcc gcagggcgat ttcgccgcga ttggcgacca
      361 ggattttctt aaacatcagc accctcgcgg ctcatcgccc gcacgctggc gcgtgccagc
      421 gcaatttgct tcgcctggcc tgcggccaga ccgccgcgat gggcgaccag gattttctta
      481 aacatcagca ccctcgcggc tcatcgcccg cacgctggcg cgtgccagcg caatttgctt
      541 cgcctggcct gcggccagac cgccgcggtt cgcgaccaga attttcttga acatccgctg
      601 cccgcagctt cttattcgat gacgaacagc ggctggccga actcgaccgc ctggccgttc
      661 tcgcccatga tccgggtgac ggtgccgctt ttttcggcct cgatctcgtt gaggatcttc
      721 atcgcttcga tgatgcacag cgtgtcgccc tccttgaccg cctgccccac ctcgacgaag
      781 ggcttggcgc ccgggctggc ggcgcggtag aaggtgccga ccatgggcga cttgacgacg
      841 aagccggtgc tttcggccgc ttcggccacc gcctgcggtg ccgcggcggc cggcgcggcg
      901 gctgccgggg gcgccgcagg cgccacggcc ggcgcggcca cgggggcgta gaccacctgg
      961 gccgccgccg gctggccgct cttgacgatg cgcaccttgc cttcggcttc ggtgatttcc
     1021 agttcagaca cgttagactc cgacaccagg tcgatcaggg tcttgagctt gcgcagatcc
     1081 atgttgctta ccaattcatt gggaacaggg tcgaatttac ctcaattcgc gtcgatttgc
     1141 gtcgaattgc cgctaatttt caattatctg gcttgcgcgc ggcatcacgc caaggccgct
     1201 ggcagccctc aggcgctgcc tgcggccttg cgccaggcgc gcagatcgcc ttcgcgcagt
     1261 tgccccagct tgcgttcgcg cagcgtgccc tgggcgtcga acagcacgct gaacggcagg
     1321 ccgccggcac tgttgcccag ctcacgcgtc aggctggcgc cctccagccc cgccagcgcc
     1381 accggaaagc tgaccggcgt ctgggccagg aaacgcccca cgggctccgg cttgtcgacc
     1441 gccaatccga gcacttgcca gccgttggcc ttgttttcgg tgtagaaggc gctcaatagc
     1501 ggcagttcgg cgatgcaggg cgggcaccag gtggcccaga agttgaccag cagcgggcgc
     1561 ccgcgcaggc cggccagcgc cagcgtgctg ccgtcggggg tggggaagct gcgctcccag
     1621 aaaccggcca gctggggttc gctcagttgc aggcgacgcg ccgcatacca ggcgccggcg
     1681 ccggccgccg ccagcgccac gccggccacc agcaggcggc gccccagcgg acgggcgggc
     1741 ggcgcaacga ccggcgctgg ctggggttcg gggagggtca tggcgtgggc tccatctgca
     1801 tgcgctgcac cagcgcctgc cggtcgccgc gcagcggctg gcccaggcgg ccgggcttga
     1861 gcgcgccgcg cagctcgtcg tggtcgtgga tcatcaggtg cacgccgatc ggctcgccca
     1921 gctccgggca ggggctggaa aagctcagtg tatccaccgg cacgccgtgc aggccgttga
     1981 tgctgcgcgc ctggtagcgc acctggtggt cgatgaggct gatttcggcc gacttgggat
     2041 cgtcgcagaa cagttgcaac cagacgtccg agcgccgcgt ggcggtgccg ttccacaccg
     2101 ccccggtgac gtgcggccgg aattcggcca gccgctccat ccagcgcaac gccagccgcc
     2161 gcagcgcggc cagctcggcc ggctgggtgt cggcatgaaa cagcgccagg taggcgcgca
     2221 cctcggcttc caccagctcg ttgtcgggca gggcggtgcg cgcgggcagg cccatgagct
     2281 tgtgggcgcg ccgcttggcg ccgccgtaat ccagcccctc ctccaccacc aggcgggcgg
     2341 cgtgggcggc caactcctgc gtcagggttt ccatgggtgt cattgtgccc ttgcgggcgc
     2401 ccggactcac ttacattcgt tcgccaatcc accggagagc cgccaatgca agccaccatg
     2461 atgtccacgc cgctgtcgct caaccacctg ttggagcgcg ccgggcggct gtttcacgac
     2521 aacaccatcg tctcgcgcct gcccgacaag agcctgcggc gccacagcta cggcgagtac
     2581 taccgccgca cgcgggcgct ggccgcggcg ctgcagggcc tgggcctgca aaagggcgac
     2641 cgcgtggcca ccctgtgctg gaaccaccac gcgcacctgg agtgctattt cggcatcccg
     2701 gccgccggcg gcgtgatgca caccctgaac ctgcgcctgc cgccagagga gatcggctgg
     2761 atcgccggcg acgcggcgga ccgctttctg atcatcgacg acgtgctgct gccgctgtac
     2821 cagcagttcg cccatctgca ccgcttcgag cgggtgatcg tgtttgcctt ctccggcgcg
     2881 ccggtggcgc ccgagttcac cgactacgag gcgctgctgg cggccgccga ccccgacgcc
     2941 ttcgactacg ccccgcacgc cgaggacgac ccgacctcca tgtgttacac ctcgggcacc
     3001 accggcaagc ccaagggcgt ggtgtattcg caccgctcca ccgtgctgca ttcgctggtc
     3061 ggcgccctgc ccgacgccac cgccgtgcgc aacaccgacg tggtgctgcc ggtcaccccc
     3121 atgttccacg ccaacagctg gggcatgccc tacatcgcgg tgatgctggg cgccaagctg
     3181 gtgtttcccg gcccgcacct gcaccccgaa gacctgctgg acctgatgca gcaggagccg
     3241 cccacgctga ccctgggggt gccgaccatc tggctggcga tgatccagct ctacgattcg
     3301 tcgctgcagc ctggctcgcc gcaccacggg cgctggcagc tgccgaccgg cctgcgctcc
     3361 atggtcggcg gcgcggcggt gcccgaaagc ctgatccgcg ccttcggccg ccacggcgtg
     3421 tggatcatgc agggctgggg catgaccgaa accagcccgg tcggcaccct ggcctacccg
     3481 cgcgccgagc tgcagggcgc cagcgacgat gaacgcttgc gccgcgccgc catggccggt
     3541 gtgccgatgc cgctggtcga cctgcgcatc cgtggcgacc acggccagga ccagccctgg
     3601 gacggcacca gcgtgggcga gttgcaggtg cgcggaccct tcatcaccgg cggctaccac
     3661 gaagccggcg cgccgcccga caaattcacc gccgacggct ggctgcgcac cggcgacgtc
     3721 gccagcgtgg acgcgctggg ctacatgcgc atcagcgacc gcaccaagga cctgatcaag
     3781 tccggcgg
//
LOCUS       DAOQTZ010000181         3751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_27693_length_3751_cov_13.087392, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000181 DAOQTZ010000000
VERSION     DAOQTZ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27693_length_3751_cov_13.087392"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..861
                     /gene="map"
                     /locus_tag="PKN51_13435"
     CDS             40..861
                     /gene="map"
                     /locus_tag="PKN51_13435"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNT86147.1"
                     /translation="MSITIKDEAGIAAMREACRLAAEVLDTLTPLIQPGITTLEIDRL
                     AAQAMAAQGTRSATVGYQPPGYPPYPAHVCTSVNQVVCHGIPADKPLKKGDIVNVDVT
                     VITPDGWYGDTSRMFEIGDCSIAARRLCALTYQAMWLGIEQVRAGARLGDIGHAIQKF
                     VEGHGLSVVREFCGHGIGQNFHEEPQVLHYGRPGTGVELEPGMIFTIEPMVNVGRRDI
                     KELPDGWTIVTKDRSLSAQWEHTVLVTPTGYEVLSLSAGAPAIPDFVKGVARAAA"
     gene            904..3558
                     /locus_tag="PKN51_13440"
     CDS             904..3558
                     /locus_tag="PKN51_13440"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNT86148.1"
                     /translation="MSERAAASCAEAPPGDYRGRKKALLDALRASGPGTRGVRAALTR
                     LSALADDALRALWAQRGFDDGPALLAVGGYGRGELFPYSDIDVLLLLPDGCDAAQDAA
                     LRARIEGFITDCWDAGLEIGSAVRSIGDCLQAGAEDVTVQTALLEARCMAGSRALFDA
                     FRQRHQAALAPLDFFSAKRLELRQRHVKYEDTPYALEPNCKESPGGLRDLQIILWTAR
                     AAGIGGRWDDLHRAGLVTAHELRQLRHNEALLSSIRCRLHLVAGRREDRLVFDLQNAV
                     AESFGLSHRLAADGRVLLRASEVLMRRYYWAAKAVTQLNQILLQNLEEHLRAAHGEPA
                     PALRRINPRFFDRDGLIEVASDELYQRQPHAVLETFLLYTQTPGVKGLSARTLRALYN
                     ARSVMNHAFRADPVNRRTFLAILQQPQGITHAMRLMNQTSVLGRTLWVFRRIVGQMQH
                     DLFHVYTVDQHILMVLRNVRRFFMAEHAHEYPFCSQLAAGWDKPWLLYVAALFHDIAK
                     GRGGDHSRLGALEVRRFCAEHGVAGADAELLEFLVREHLSMSRIAQSEDLSDPEVIAA
                     FARQVGNERQLTALYLLTVADLRGTSPKVWNAWKGKLLEDLYRLTVRSLGGRAPDADA
                     VIEARKREALRELTPAGGPPALPPPLWEQLQVSYFMRHDAGDIAWHARELSPALAGSA
                     ATAPDQPLRATTVRARLSPVGEGLQVLVHASDRPDLFARICGWFERSGFSILEAKIHT
                     TRDVWALDTFQIVRPEAPEDYAEFIDAVQAELPPAIDSQGPLPAPRLGRLSRRVRSFP
                     VAPRVDLRPDEKGQRWLLSVTASDRMGLLYGIARVLAAHQIDVELARITTLGERVEDS
                     FLLQGAQLQHARTQLAIEQELLDVLEPA"
BASE COUNT          561 a         1391 c         1278 g          521 t
ORIGIN      
        1 cgcaggcgcg tcgcgcgccc ctctgggata atttgcccca tgtcaatcac catcaaagac
       61 gaagccggca tcgccgccat gcgcgaagcc tgccgcctgg cggccgaggt gctggacacc
      121 ctgacgccgc tgatccagcc gggcatcacc acgctcgaaa tcgaccgcct ggccgcccag
      181 gccatggccg cccagggcac gcgctcggcc accgtcggct accagccgcc cggctatccg
      241 ccctacccgg cgcatgtgtg cacctcggtc aaccaggtgg tgtgccacgg catcccggcc
      301 gacaagccgc tcaagaaggg cgacatcgtc aacgtcgacg tcaccgtcat cacgcccgac
      361 ggctggtacg gcgacaccag ccgcatgttc gagatcggcg actgctcgat cgccgcccgc
      421 cgcctgtgcg ccctgaccta ccaggcgatg tggctgggca tcgagcaggt gcgcgccggc
      481 gcccgcctgg gcgacatcgg ccacgcgatc cagaagttcg tcgaaggcca cggcctgtcg
      541 gtggtgcgcg agttctgcgg ccacggcatc ggccagaact tccacgaaga gccgcaggtg
      601 ctgcactacg gccgccccgg caccggggtg gagctggagc cgggcatgat cttcaccatc
      661 gagcccatgg tcaacgtcgg ccggcgcgac atcaaggaac tgcccgacgg ctggaccatc
      721 gtcaccaagg accgcagcct gtcggcgcag tgggagcaca cggtgctggt cacgcccacc
      781 ggctacgagg tgctgagcct gtcggccggc gcgccggcga ttcccgattt cgtcaagggc
      841 gtggcgcgcg ccgccgcctg agcacggatc aaccctccgc cccaccttct gaacccgtcc
      901 cgcatgtccg agcgcgccgc cgcctcctgc gccgaggcgc cacccggcga ctaccggggg
      961 cgcaagaagg cgctgctgga cgcgctgcgc gccagcggcc ccggcacacg cggcgtgcgc
     1021 gccgccctca cccgcctgag cgcgctggcc gacgacgcgc tgcgcgcgct gtgggcgcag
     1081 cgcggcttcg acgatgggcc ggccctgctg gcggtgggcg gctacggccg cggcgagctg
     1141 ttcccctatt ccgacatcga cgtgctgctg ctgctgccgg acggctgcga tgcggcgcag
     1201 gacgcggcgc tgcgcgcgcg catcgaaggc ttcatcaccg actgctggga cgccgggctg
     1261 gaaatcggct cggcggtgcg cagcatcggc gactgcctgc aggccggcgc cgaagacgtg
     1321 acggtgcaga ccgcgctgct ggaggcgcgc tgcatggccg gcagccgcgc actgttcgac
     1381 gccttccgcc agcgccacca ggcggcgctg gcgccgctgg acttcttcag cgccaagcgg
     1441 ctggagctgc gccagcgcca cgtcaagtac gaagacacgc cctacgccct ggagcccaac
     1501 tgcaaggaat cgcccggcgg cctgcgcgac ctgcagatca tcctgtggac ggcgcgcgcc
     1561 gccggcatcg gcgggcgctg ggacgacctg caccgcgccg gcctggtgac cgcgcacgag
     1621 ctgcgccagc tgcgccacaa cgaggcgttg ctgtccagca tccgctgtcg cctgcacctg
     1681 gtggccgggc ggcgtgaaga ccggctggtg ttcgacctgc agaacgcggt ggccgaatcc
     1741 ttcggcctga gccaccgcct ggccgccgac ggccgcgtgc tgctgcgcgc cagcgaggtg
     1801 ctgatgcggc gctactactg ggccgccaag gcggtgaccc agctcaacca gattctgctg
     1861 cagaacctgg aagagcacct gcgcgccgcc cacggcgagc cggcgccggc gctgcgacgc
     1921 atcaacccgc gcttcttcga ccgcgacggc ctgatcgaag tcgccagcga cgagctctac
     1981 cagcgccagc cgcacgcggt gctggaaacc tttctgctct acacccagac gcccggcgtc
     2041 aagggcctgt cggcgcgcac cctgcgcgcg ctctacaacg cgcgcagcgt gatgaaccac
     2101 gccttccgcg ccgacccggt caatcgccgc accttcctgg ccatcctgca gcagccgcag
     2161 ggcatcaccc acgccatgcg gctgatgaac cagacttccg tgctggggcg cacgctgtgg
     2221 gtatttcggc gcatcgtcgg gcagatgcag cacgacctgt tccacgtcta cacggtggac
     2281 cagcacatcc tgatggtgct gcgcaacgtg cggcgcttct tcatggccga gcacgcgcac
     2341 gaatacccgt tctgctcgca gctggcggcc ggctgggaca agccctggct gctgtacgtg
     2401 gcggcgctgt tccacgacat cgccaagggg cgcgggggcg accactcgcg cctgggcgcg
     2461 ctggaggtgc ggcgcttctg cgccgagcac ggcgtggccg gcgccgacgc cgaactgctc
     2521 gaatttctgg tgcgcgagca cctgagcatg agccgcatcg cgcagagcga ggacctgagc
     2581 gaccccgagg tgatcgccgc cttcgcgcgc caggtcggca acgaacgcca gctcaccgcg
     2641 ctgtacctgc tgaccgtggc cgacctgcgc ggcaccagcc ccaaggtgtg gaacgcctgg
     2701 aagggcaagc tgctggaaga cctgtaccgc ctgacggtgc gcagcctggg cgggcgagcg
     2761 cccgacgccg acgccgtgat cgaggcgcgc aagcgcgagg cgctgcgcga gctcaccccc
     2821 gccggcggcc cgcccgccct gccgccaccg ctgtgggagc agttgcaggt cagctacttc
     2881 atgcgccacg acgccggcga catcgcctgg catgcgcgcg agctgtcgcc cgccctcgcc
     2941 ggcagcgcgg ccacggcgcc agaccagccg ctgcgtgcca ccacggtgcg cgcccgcctg
     3001 tcgccggtgg gcgaagggct gcaggtgctg gtgcacgcca gcgaccggcc ggacctgttc
     3061 gcgcgcatct gcggctggtt cgagcgcagc ggcttttcca tcctggaggc caagatccac
     3121 accacgcgcg acgtctgggc gctggacacc ttccagatcg tgcgccccga ggcgcccgag
     3181 gactacgccg agttcatcga cgccgtgcag gccgagctgc cgcccgccat cgacagccag
     3241 gggccgctgc cggcgccgcg cctggggcgc ttgtcgcggc gcgtcaggag ctttccggtg
     3301 gccccgcggg tggacctgcg gcccgacgaa aagggccagc gctggctgct gtcggtcacc
     3361 gccagcgatc gcatgggcct gctgtatggc attgcccgcg tgctggcggc gcaccagatc
     3421 gacgtcgagc tggcccggat caccaccctg ggcgagcgcg tggaagacag ctttctgctg
     3481 caaggggccc agctgcagca cgcacgcacc cagctggcca tcgagcagga actgctggac
     3541 gtgctggagc cggcctgaca actcctgttt cgatagctgc tggcgcttgc tgcataaggc
     3601 gtggaggcgc aaaaagcact tgaccgacac cggccacggc accgcgcccg ccgccccggc
     3661 ggccagtgca gtgtttcgcg cggtcaggca cttgtagaag cctttttctg gcttcccgca
     3721 gggcgagccg ccaaggggcc atctgctttg t
//
LOCUS       DAOQTZ010000182         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_28698_length_3662_cov_12.188800, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000182 DAOQTZ010000000
VERSION     DAOQTZ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28698_length_3662_cov_12.188800"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKN51_13445"
     CDS             complement(<1..234)
                     /locus_tag="PKN51_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNT86149.1"
                     /translation="MAEFKEISPNAPLGAKLHNWFDNRFPTLFAEYRKHMSEYYAPKN
                     FNFWYFFGSLAMLVLAIQIVTGIFLVMHYKPDAA"
     gene            complement(247..876)
                     /gene="petA"
                     /locus_tag="PKN51_13450"
     CDS             complement(247..876)
                     /gene="petA"
                     /locus_tag="PKN51_13450"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNT86150.1"
                     /translation="MSDVPASTFTPAPMDSSKRTWLIASSCAGAAGAAALATPFVTSF
                     APSERAKAAGAAVEVDIASLKPGEKLTVEWRGKPVWIMRRTPEQMATLKGLDGQLADP
                     NSERTAFPTPDYARNEARARQEHPELLVVVGICTHLGCSPVDKFQTGAQPSLPDDWKG
                     GFFCPCHGSTFDLAGRVFKNKPAPDNLEVPPYAYLSDARLLIGEDKKQG"
     gene            1031..1453
                     /gene="mscL"
                     /locus_tag="PKN51_13455"
     CDS             1031..1453
                     /gene="mscL"
                     /locus_tag="PKN51_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNT86151.1"
                     /translation="MGMMQEFREFAVKGNAIDLAVGVIIGGAFGKIVDSIVGDLIMPV
                     VGKLFGGLDFSNYYIALAGQAAGLSLEEAKKGGAVFAYGNFITILVNFILLAFIIFMM
                     VKQINRLKKEEPAAAEPAPAEPAEEVQLLREIRDSLKR"
     gene            complement(1524..2549)
                     /gene="pdxA"
                     /locus_tag="PKN51_13460"
     CDS             complement(1524..2549)
                     /gene="pdxA"
                     /locus_tag="PKN51_13460"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNT86152.1"
                     /translation="MNKPLAITMGDGAGIGPETIAKAFRDAPDLLRGCFVAGDLATLR
                     RAAAVVSEGGVSLPVAALEQPAEALAAPPRCVPVLQVVEPRVPAPFGRISAPAGRAAG
                     DCVVWAARAALRGELAGLVTAPLHKEALAAAGAPYDRYPGHTELLQAVAAEHLGVPVA
                     RLPVRMMLANDELRTVLVSIHVSLSEAIEQVSSGNIAETLRITHEAVAAALQRPPRIA
                     VAGLNPHAGEGGLFGREEIEVIAPAIAAARARGIDAHGPFAPDTVFMRARGFAEFDVV
                     VAMYHDQGLIPVKYLGVEQGVNVTLGLPLVRTSPDHGTAFDIAGSGRADAASLIAAVR
                     MARQLAP"
     gene            complement(2633..3409)
                     /locus_tag="PKN51_13465"
     CDS             complement(2633..3409)
                     /locus_tag="PKN51_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNT86153.1"
                     /translation="MSALLPSIDRAALLAACNAELRPEQFKDYGPNGLQVEGKPRIGK
                     IVSGVTASRALIDAAIAAGADALFVHHGLFWRGQDGTVTGWMKQRLQRLLAHDINLLA
                     YHLPLDAHPTLGNNAQLGQRLGLLATGRFGEQELGFIGHPAAGPGFDDAQALAHHLEH
                     VLNRPVTQVGQAQTAIKNIAWCSGGAQGYFEAAIAAGAQAFITGEISEPQAHLARECG
                     VVFYACGHHATERYGAPAMAAHVAARLGIAHQFIEIDNPA"
     gene            3437..>3662
                     /locus_tag="PKN51_13470"
     CDS             3437..>3662
                     /locus_tag="PKN51_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86154.1"
                     /translation="MACDESTLPGVHARFAAPSMPGPRMVFQGKKQNAGLPTRKENAM
                     RKITWTFWLLIGGLTALWLLADTLWPAQAGL"
BASE COUNT          568 a         1198 c         1280 g          616 t
ORIGIN      
        1 ggcggcgtcg ggcttgtagt gcatcaccag gaagatgccg gtgacgatct ggatcgccag
       61 caccagcatg gccagcgagc cgaagaagta ccagaagttg aagttcttcg gcgcgtagta
      121 ctccgacatg tgtttgcggt attccgcaaa cagcgtcggg aaccggttgt cgaaccagtt
      181 gtgcagcttg gcacccagcg gggcgttggg ggagatttcc ttgaattcag ccatgaccgc
      241 ctctgtttat ccttgtttct tgtcttcgcc gatcagcagc ctggcgtcgg agagataggc
      301 gtagggcggc acttcaaggt tgtcgggcgc gggcttgttc ttgaacacgc gaccggccag
      361 gtcgaaggtg gagccatggc aggggcagaa gaagccgccc ttccagtcgt cgggcagcga
      421 gggctgggcg ccggtctgga acttgtcgac cggcgagcag cccaggtggg tgcagatgcc
      481 gaccaccacc agcagttcgg ggtgctcctg gcgggcgcgc gcctcgttgc gggcgtagtc
      541 gggcgtgggg aaggcggtgc gctcggagtt cgggtcggcc agctggccgt cgaggccctt
      601 cagcgtcgcc atctgctccg gcgtgcggcg catgatccag accggcttgc cgcgccattc
      661 cacggtcagc ttctcgcccg gcttgaggct ggcgatatcg acctcgaccg ccgcgcccgc
      721 cgccttggcc cgctcggacg gcgcgaagct ggtgacgaag ggggtggcca aggccgcggc
      781 gccagccgcg cccgcgcaac tggacgcgat cagccacgtg cgcttgctgc tgtccatggg
      841 tgcgggggtg aaggtgcttg cagggacatc actcatgggg ggttcctctg gcaggttttt
      901 ttatctcgta atcaacggtc gattgtagcg gagcgtcttc ggcgcaccgc ccgccatccg
      961 gcgcaggatt gacacggatc agccgccggc aatactgtgg ccctattcaa caacctcgaa
     1021 ggggatgcgc atgggcatga tgcaggagtt cagagagttc gccgtcaagg gcaacgccat
     1081 cgatctggcg gtgggcgtga tcatcggcgg cgcgttcggc aagatcgtcg actcgatcgt
     1141 tggcgatttg atcatgccgg tggtcggcaa gctgttcggc ggactggatt tttccaatta
     1201 ctacatcgcg ctggccggcc aggcggcggg cctgagcctg gaagaggcca agaagggcgg
     1261 tgcggtgttc gcctacggca acttcatcac catcctggtc aacttcatcc tgctggcctt
     1321 catcatcttc atgatggtca agcagatcaa ccggctgaag aaggaagaac cggccgccgc
     1381 cgagccggcg ccggccgaac cggcggaaga ggtgcaactg ctgcgcgaga tccgcgacag
     1441 tctgaagcgc tgagtccgta ccgttcgata gctatctaat ttatagcctc tggcgcccgt
     1501 ccgcactgcg cggcgggcgt ttttcatggt gcaagctggc gcgccatgcg cacggccgcg
     1561 atgaggctgg cggcgtccgc ccggccgctg ccggcgatgt cgaacgccgt gccgtggtcg
     1621 gggctggtgc gcaccagggg caggcccagc gtgacgttga cgccctgctc cacccccagg
     1681 tacttgaccg ggatcaggcc ctggtcgtgg tacatggcca ccaccacgtc gaactcggcg
     1741 aagccgcgcg cgcgcatgaa cacggtgtcg ggcgcgaagg ggccgtgcgc gtcgatgccg
     1801 cgcgcgcggg cggcggcgat cgccggggcg atgacctcga tttcctcgcg cccgaacagc
     1861 ccaccctcgc cggcgtgcgg gttcagcccg gcgaccgcga tgcgcggcgg ccgctgtagc
     1921 gccgccgcca cggcctcgtg cgtaatgcgc agggtttccg cgatgttgcc actgcttacc
     1981 tgctcgatgg cttcgctcag cgagacgtgg atgctgacca gcacggtgcg cagctcgtcg
     2041 ttggccagca tcatgcgcac cggcagccgc gccaccggca cgcccaggtg ctcggccgcc
     2101 acggcttgca gcagctcggt gtggcccggg tagcggtcgt agggcgcgcc ggcggcggcc
     2161 agcgcctcct tgtgcagcgg ggcggtgacc aggcccgcca gctcgccacg cagcgccgcg
     2221 cgcgcggccc agaccacgca gtcgccggcg gcgcggccgg ccggcgcgct gatgcgcccg
     2281 aacggcgccg gcacccgcgg ctcgaccacc tgcagcaccg gcacgcagcg cggcggcgcc
     2341 gccagcgcct cggccggctg ctccagcgcg gccacgggca ggctcacgcc gccttcgctc
     2401 accacggccg cggcgcgccg cagcgtggcc aggtcaccgg cgacaaagca accgcgcagc
     2461 agatcgggcg cgtcgcggaa cgccttggcg atggtttccg gcccgatgcc ggcgccgtcg
     2521 cccatggtga tggccagtgg tttattcatg gtcaaattcg gctgtaacca gcacccaaac
     2581 tgcgccagca gctatgtttt taaaagcatc tggcatcgct gcaagagcgc gctcatgccg
     2641 ggttgtcgat ctcgatgaac tggtgcgcga tccccagccg cgccgccaca tgggccgcca
     2701 tggccggggc gccgtagcgc tcggtggcgt ggtggccgca ggcgtagaac accacgccgc
     2761 actcgcgcgc cagatgcgcc tgcggctccg agatttcgcc ggtgatgaag gcctgcgccc
     2821 cggcggcgat ggcggcttcg aagtagccct gcgccccgcc gctgcaccaa gctatatttt
     2881 taatagctgt ttgcgcttgc cctacctgcg tgacaggccg attcagtaca tgctccagat
     2941 gatgcgccag cgcctgcgcg tcgtcgaagc cggggcccgc ggccggatgg cctatgaagc
     3001 ccagctcctg ctcgccgaag cgcccggtgg ccagcaggcc caggcgctgg cccagctggg
     3061 cgttgttgcc cagcgtgggg tgggcgtcca gcggcaggtg gtaggccagc aggttgatgt
     3121 cgtgcgccag caggcgctgc aggcgctgct tcatccagcc ggtgacggtg ccgtcctggc
     3181 cgcgccagaa caggccgtgg tgcacaaaaa gggcgtcggc accggcggcg atggcggcgt
     3241 cgatcagggc ccggctggcg gtgacgccgc tgacgatctt gccgatgcgc ggcttgccct
     3301 ccacctgcag gccgttgggg ccgtagtcct tgaactgctc ggggcgcagc tccgcgttgc
     3361 aagccgccag cagcgcggca cggtcgatac tgggaagcag tgctgacatg gcggcattgt
     3421 cgaccatggg ccgcccatgg cctgcgacga atcgacttta cccggcgttc acgcaaggtt
     3481 cgcagcgcct tcaatgccgg ggccgagaat ggtgttccaa gggaaaaaac agaacgcggg
     3541 tctccccacc cgcaaagaaa acgcgatgag aaaaatcacc tggactttct ggttgctgat
     3601 cggcggtctc accgccctgt ggctgctggc cgacacgctg tggccggcgc aggccggctt
     3661 gt
//
LOCUS       DAOQTZ010000183         3625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_29120_length_3625_cov_14.090196, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000183 DAOQTZ010000000
VERSION     DAOQTZ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29120_length_3625_cov_14.090196"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..1475
                     /locus_tag="PKN51_13475"
     CDS             354..1475
                     /locus_tag="PKN51_13475"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNT86155.1"
                     /translation="MNAKAVHAIESWQTSPERTSRTDDARIKDITVLPPPDHLIRFFP
                     IRGTPVESLITDTRQAIRRIINGKDDRLLVVVGPCSIHDPVAALDYARRLKPLREHYA
                     DTLEVVMRVYFEKPRTTVGWKGLINDPYLDESYRIDEGLRIARQLLIDINRLGLPAGS
                     EFLDVISPQYIGDLISWGAIGARTTESQVHRELASGLSAPIGFKNGTDGNIRIATDAI
                     QAAARGHHFLSVHKNGQVAIVHTDGNKDCHVILRGGKAPNYDAASVDTACKELAASKL
                     HASLMVDCSHANSSKQHEKQLDVARDVAAQVSAGSRGVFGVMVESHIHGGAQKFTPGK
                     DQVASLEYGKSITDACIGWDGTQTILQVLSDAVRARRGR"
     gene            1584..1895
                     /locus_tag="PKN51_13480"
     CDS             1584..1895
                     /locus_tag="PKN51_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86156.1"
                     /translation="MQSEVRVIQDERTEWRFDLDHAAPMDNAQARAWLDAQFIQLGGE
                     PLRPTGKLLLADKVLVVAREAGARRLDDPQWGQDFARAASAALAKPVVRIDLAAMTVS
                     Y"
     gene            complement(1944..3359)
                     /gene="mpl"
                     /locus_tag="PKN51_13485"
     CDS             complement(1944..3359)
                     /gene="mpl"
                     /locus_tag="PKN51_13485"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNT86157.1"
                     /translation="MHIHILGICGTFMGGLAALAREAGHRVTGCDAGVYPPMSDQLRA
                     LGIELIEGYGADQLALRPDMFVIGNVVSRARLPDGSPKYPLMEAILDAGAPYTSGPQW
                     LAEHVLAGRHVLAVAGTHGKTTTTSMLAWILESAGLSPGFLVGGVPLNFGISARLQGQ
                     APGQPRPLFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDLAAIERQF
                     HHLVRTVPPSGRVIVNDLEESLTRVLAQGCWSERRGFGGAISDFMALGEASDFEVQQR
                     GRPVGRVRWSLSGQHNQLNALAAIAAAEHVGVDPATACAALAQFQNVRRRMEVRGRVA
                     VAGGEVTVYDDFAHHPTAIRTTVDGLRRQLDAAGQAGQRILAVFEPRSNTMKLGTMKS
                     QLPWALEHADLSVCHTGGLDWDAAAALSPLGARAQLAGSIDEVVQQVSQAARAGDHVL
                     CMSNGGFGGVHGRLLESLQKR"
BASE COUNT          605 a         1279 c         1204 g          537 t
ORIGIN      
        1 aataccggcc tgtcacgccg ggggtcgcgg gttcgagtcc cgtccactcc gccaattcac
       61 gcacaaaagc ggcccgaggg ccgcttttgt tttgcccacg ctttctgcga cgcgccgtcg
      121 catgcatgcg acaggttccg ggccgagggc gtggtatatt ttgaatgtga tgagcaagcc
      181 cgtcgccgct ttcggctttt atttttggtt ctctgtcccc ggcggacgag aggaaagcct
      241 gtaagtcaac gcgatcagcg acctcccgaa acaaccgccg cagcgagccc gcccggcggt
      301 ttttttgtgc cccatcgccc accgatccat ctctttctca aggaagcaca ccgatgaacg
      361 ccaaagccgt ccatgccatc gagtcctggc agacgtcgcc ggaacgcacc agccgcaccg
      421 acgacgcacg catcaaggac atcaccgtgt tgcccccacc cgatcacctg atccgcttct
      481 tccccatccg cggcacgccg gtcgagtcgc tcatcaccga tacgcgccag gccatccgtc
      541 gcatcatcaa cggcaaggac gaccgcctgc tggtggtggt ggggccgtgc tccatccacg
      601 acccggtggc ggcgctggac tatgcgcgcc ggctcaagcc gctgcgcgaa cactacgccg
      661 acacgctgga ggtggtgatg cgggtgtact tcgaaaaacc ccgcaccacg gtgggctgga
      721 agggcctgat caacgaccct tacctggacg agagctaccg catcgacgag ggcctgcgca
      781 tcgcgcgcca gctgctgatc gacatcaacc gcctgggcct gccggcgggc agcgagttcc
      841 tcgatgtcat ctcgccccag tacatcggcg acctgatcag ctggggcgcg atcggcgcgc
      901 gcaccaccga aagccaggtg caccgcgagc tggccagcgg cctgtcggcg ccgatcggct
      961 tcaagaacgg caccgacggc aacatccgca tcgccaccga cgccatccag gcggcggcgc
     1021 gcgggcacca ctttctgtcg gtgcacaaga acggccaggt ggccatcgtg cacaccgacg
     1081 gcaacaagga ctgccacgtc atcctgcgcg gcggcaaggc gcccaactac gacgccgcca
     1141 gcgtcgacac cgcctgcaag gagctggcgg ccagcaagct gcacgcctcg ctgatggtcg
     1201 attgcagcca cgccaacagc agcaagcagc acgaaaagca gctcgacgtg gcgcgcgacg
     1261 tggcggcgca ggtcagcgcc ggctcgcgcg gcgtgttcgg tgtgatggtg gaaagccaca
     1321 tccacggcgg ggcgcagaag ttcacgcccg gcaaggacca ggtggccagc ctggagtacg
     1381 gcaagagcat caccgacgcc tgcatcggct gggacggcac gcagaccatc ctgcaggtgc
     1441 tgtccgacgc cgtgcgggcg cgacgcggcc gctgaggccg gtcaaggtcg gccgccgccg
     1501 atcgactaca gtcggtgggc gcgcggcacg cggcatggcg ccgccacccg cgcacccgtc
     1561 agaactcagg aggccagccc atcatgcaga gtgaagtacg cgtcatccag gacgaacgca
     1621 ccgaatggcg attcgacctc gaccacgccg cccccatgga caacgcccag gcccgcgcct
     1681 ggctggacgc gcagttcatc cagctgggcg gcgagccgct gcgccccacg ggcaagctgc
     1741 tgctggccga caaggtgctg gtggtggcgc gcgaagccgg cgcgcgtcgg ctggatgacc
     1801 cgcagtgggg ccaggacttc gcccgtgccg ccagcgccgc gctggccaag ccggtggtgc
     1861 gcatcgatct ggcggcgatg acggtgtcgt actgaatcgc aagtcaaatc aggcgataac
     1921 gcctgtccag cgcgcgccag cagctatctt ttttgaagcg attcaagcag ccggccgtgc
     1981 acgccgccga agccgccgtt gctcatgcac agcacgtggt caccggcccg cgcggcctgg
     2041 ctgacctgct gcacgacctc atcgatgctg cctgccagct gggcgcgcgc gcccagcggt
     2101 gacagcgccg ctgcggcatc ccagtccagc ccgccggtgt ggcagacgct caggtcggcg
     2161 tgttccagcg cccagggcag ttgcgacttc atggtgccca gcttcatggt gttgctgcgc
     2221 ggctcgaaca ccgccaggat gcgctgcccg gcctggccgg cggcgtcgag ctggcggcgc
     2281 aggccgtcga ccgtggtgcg aatggcggtc ggatggtggg caaagtcgtc gtacaccgtc
     2341 acctcgccgc ccgccaccgc cacgcggccg cgcacctcca tgcgccggcg cacgttctgg
     2401 aactgcgcca gcgccgcgca ggcggtggcc ggatcgaccc ccacgtgctc ggccgcggca
     2461 atcgcggcca gcgcattgag ctggttgtgc tgacccgaca gcgaccagcg cacgcggccg
     2521 acggggcgcc cgcgctgctg cacctcgaag tccgaggcct cgcccagcgc catgaaatcg
     2581 ctgatcgcgc caccaaagcc gcgccgctcg ctccagcagc cctgggccag cacccgcgtc
     2641 aggctttctt ccaggtcgtt gacgatgacg cggcccgaag gcggcacggt gcgcaccagg
     2701 tggtggaact ggcgctcgat cgccgccagg tcgtcaaaga tgtcggcgtg gtcgaactcc
     2761 aggttgttca gcaccgccgt gcgcgggcgg tagtgcacga acttgctgcg cttgtcgaag
     2821 aaggcggtgt cgtattcgtc cgcctcgatg acgaacagcg gtcgcggctg gccgggggcc
     2881 tgaccctgca aacgcgccga aatgccgaag ttcagcggca ccccgcccac cagaaagccg
     2941 ggtgacagcc cggcgctttc cagaatccac gccagcatcg acgtggtggt ggtcttgccg
     3001 tgcgtgccgg ccaccgccag cacgtggcgg ccggccagca catgctcggc cagccactgc
     3061 gggccgctgg tgtagggcgc gccggcgtcg aggatcgcct ccatcagcgg gtacttgggc
     3121 gaaccgtcgg gcagccgcgc ccggctcacc acgttgccga tcacgaacat gtcgggccgc
     3181 agcgccagct ggtcggcgcc gtagccctcg atcagctcga tgcccagcgc gcgcagctgg
     3241 tcgctcatcg gcgggtagac gccggcgtcg cagccggtga cgcggtggcc ggcctcgcgc
     3301 gccagcgccg ccagaccgcc catgaaggtg ccacagatgc ccagaatgtg aatgtgcatg
     3361 gcgcgcgatt ctaggccgct ttgccgccgg ctcgctgcgg tgttgagaac gtttttggcc
     3421 tctggcgccc aatcggcaag cgccagcagc tatgctaacg atagcgtccg tggggtggct
     3481 gggcgccccg gcggccgtgt tggctgcgcc ggccaggtgg ctgtcggccc tgaagcgccg
     3541 acagcgagag tcggccccag cgagaccacg tgttgccgac cagcgcttga gaagtggaaa
     3601 accgttgtgg aagcccttgt agccc
//
LOCUS       DAOQTZ010000184         3555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_29935_length_3555_cov_15.134286, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000184 DAOQTZ010000000
VERSION     DAOQTZ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29935_length_3555_cov_15.134286"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKN51_13490"
     CDS             <1..275
                     /locus_tag="PKN51_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNT86158.1"
                     /translation="ELNRRKEPLEAYPLDAMTMVGSLDKGGQKVALLKVENLLHQVRK
                     GNYLGQNYGRVTQITESEITLREIVQDAAGEWIERGTTLQLQENTK"
     gene            326..2431
                     /gene="pilQ"
                     /locus_tag="PKN51_13495"
     CDS             326..2431
                     /gene="pilQ"
                     /locus_tag="PKN51_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNT86159.1"
                     /translation="MLLWGAAGLSLAEPRIQAVSSSIQGGTEVVKIDLSEPLAAVPAG
                     FLVQSPARIALDFPGVGSDVQRSMIELKHGNLRGAQVVQAADRTRVVLSLQQPANYRA
                     EVQGNSVLVSLDQVVTASAAAAAPARTFAESGNVDALPLRDIDFRRGADSTGRVVVDL
                     ANNQVGVDIRQQGQNLVVEFLRTSLPEGLRRKLDVSDFGTPVQTITTVQSGDRVRMVI
                     EPRGLWEHSAYQSDNQLVVEVRQQKVSADKLTQGPGYRGEKLSLNFQNIDVRSLLQVI
                     ADFTNFNIVTSDTVQGNLTLRLKDVPWDQALDIILQAKSLGMKRHGNVLQIAPKDELA
                     AKEKAELEAQKAIRDLEPLRTQSFQLNYTKAEDIKQQLTSTSGGGGGTGGGSEGRLLS
                     SRGSVIAEPRTNQLFVSDIPSRLEQIQDMIAKLDVPVRQVLIEARIVEASDTFGKSLG
                     VKLGGADLRGIRGGDAGYGLGSSGNRVAFGGNYNAITSTTGQTDASGLTSLTSQFVNL
                     PASGLAGATPASFAISLFSSAANRFLNLEISALEADGKGRVVSSPRVVTADQTKALIE
                     QGTEFPYEQATSSGATSIAFRKAVLKLEVTPQITPEGNIILDLDVNKDSPGTEVRGGM
                     SINTKRVKTQVLVENGGTVVIGGIFELTERESENKVPVLGDVPVLGNLFKQRTRQAEK
                     QEMLVFITPKMISDRGAVR"
     gene            2483..>3555
                     /locus_tag="PKN51_13500"
     CDS             2483..>3555
                     /locus_tag="PKN51_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86160.1"
                     /translation="MRKLIGWVGLAAATLLTACGGGGGSPGDTPESYTITLRAAKTSL
                     PLNVAHYPAGIGVDAPFTTTLYVEAKKGDAPIPGGEDIFACNTSYGLDSGPLYYLDGD
                     DEHEDDDGNPLAYRNITLGSNAGGNSFHFHAGNQAGVATITCSVHDPRANREVSASVD
                     ITVGGGSGTGMAASIRGVAQHATLGTQGNLNNLRTSTAISAYVWDDANQPVPSNGKSN
                     VQASIVGSTAPGAPAWGARLLAGAQQGSTVQVSTTGGVGLFSLSSGPNRGPILLELKA
                     DRRDNDVSNGIQDEVRHLLVVWAVEGVAATPLSLKPVEFDVPNATPFGAVLSVTGGEA
                     PYTWTALDALPTGLSLSSGGVLQG"
BASE COUNT          657 a         1197 c         1188 g          513 t
ORIGIN      
        1 ccgagctgaa ccgccgcaag gagccgctgg aagcctatcc gctggacgcc atgaccatgg
       61 tgggcagcct ggacaagggc ggccagaagg tggcgctgct gaaggtcgag aacctgctcc
      121 atcaggtgcg caaaggcaat tacctgggtc agaactacgg gcgcgtgacg cagatcactg
      181 aaagcgaaat caccctgagg gaaatcgttc aggacgcggc gggtgaatgg atcgagcgcg
      241 gcaccacgct gcagctccag gagaacacga aatgaggaag tgggtgaaat ttctgctgcc
      301 gcgcgcgggc gttgcgggtg tcggcatgct gctctggggc gctgccgggc tgagcctggc
      361 cgagccgcgc atccaggccg tctccagttc gatccagggt ggcaccgagg tggtcaagat
      421 cgacctcagc gaacccctgg cggcggtgcc ggcgggcttc ctggtccagt cgcccgcgcg
      481 catcgcgctc gacttccccg gggtgggcag cgacgtgcag cgcagcatga tcgagctcaa
      541 gcatggcaac ctgcgcggcg cgcaggtggt gcaggcggcc gatcgcacgc gcgtggtgct
      601 gagcctgcag cagccggcca actaccgcgc cgaagtgcag ggcaactcgg tgctggtgtc
      661 gctcgaccag gtcgtgacgg cgtcggccgc cgcggccgcg ccggcgcgca cctttgccga
      721 aagcggcaac gtcgacgccc tgccgctgcg cgacatcgac ttccgacgcg gtgccgacag
      781 caccggccgg gtggtggtcg acctggccaa caaccaggtg ggggtggata tccgccagca
      841 gggccagaac ctggtggtcg agttcctgcg caccagcctg cccgaaggcc tgcgccgcaa
      901 gctcgacgtg tcggatttcg gcaccccggt gcagaccatc accacggtgc aaagcggtga
      961 tcgggtgcgc atggtgatcg agccgcgcgg actgtgggag cacagcgcct accagagcga
     1021 caaccagctg gtggtcgaag tgcgccagca gaaggtcagc gccgacaagc tgacccaggg
     1081 tccgggctac cgcggtgaaa aactgtcgct caacttccag aacatcgacg tgcgctcgct
     1141 gctgcaggtg atcgccgact tcaccaactt caacatcgtc acctccgaca ccgtgcaggg
     1201 caacctgacc ctgcgcctga aggacgtgcc gtgggatcag gcgctcgaca tcatcctgca
     1261 ggccaagagc ctgggcatga agcgccatgg caacgtgctg caaatcgcac ccaaggacga
     1321 gctggcggcc aaggaaaagg ccgagctcga agcgcagaag gccatccgtg acctggagcc
     1381 gctgcgcacc cagtcgttcc agctcaacta caccaaggcc gaggacatca agcagcagct
     1441 cacctccacc tcgggtggtg gcggtggcac cggcggcggc agcgaaggtc gcctgctcag
     1501 ctcgcgtggc agcgtgatcg ccgagccgcg caccaaccag ctgttcgtgt ccgacatccc
     1561 gtcgcgcctg gagcagattc aggacatgat cgccaagctc gacgtgccgg tgcgccaggt
     1621 gctgatcgag gcgcgcatcg tcgaagcctc cgacactttc ggcaagtcgc tgggcgtcaa
     1681 gctcggtggc gccgacctgc gcggcatccg tggcggcgac gccggctacg gcctgggcag
     1741 cagcggcaac cgggtggcct tcggcggcaa ctacaacgcc atcacctcca ccaccgggca
     1801 gaccgacgcc tcgggcctga cttcgctgac ctcgcagttc gtcaacctgc cggcctcggg
     1861 cctggccggc gccacgccgg cgagctttgc catctcgctg ttcagctcgg ccgccaaccg
     1921 cttcctgaac ctggaaatct cggccctgga agccgacggc aagggccgtg tggtgtccag
     1981 cccgcgcgtg gtcaccgccg accagaccaa ggcgctgatc gagcagggca cggaattccc
     2041 gtacgagcag gccacatcca gcggcgccac ctcgatcgcc ttccgcaagg cggtgctcaa
     2101 gctggaagtc acgccgcaga tcacgcccga gggcaacatc atcctcgacc tcgacgtcaa
     2161 caaggacagc cccggcaccg aggtgcgcgg tggcatgtcg atcaacacca agcgcgtcaa
     2221 gacgcaggtg ctggtcgaaa atggcggcac ggtggtgatc ggcggcatct tcgagctgac
     2281 cgagcgcgag agcgagaaca aggtgccggt gctgggcgat gtgcccgtgc tgggcaactt
     2341 gttcaagcag cgcacgcgcc aggccgaaaa gcaggaaatg ctggtgttca tcacccccaa
     2401 gatgatctcg gatcgcggtg cggtgcgctg agcgcctcca ggaaacaggg cggaaactct
     2461 cttttttaac caggttgacc ttatgcgaaa gttgattgga tgggtggggc tggcagcggc
     2521 cacgttgctg acggcatgcg gtggtggcgg cggcagcccg ggtgacaccc cggagtccta
     2581 caccatcacg ctgcgtgctg ccaagaccag tctgccgctg aacgtggccc actacccggc
     2641 gggcatcggc gtcgacgcgc cgttcaccac cactctctat gtggaagcca agaagggcga
     2701 tgcgccgatt cccggcggtg aggacatctt tgcctgcaac accagctacg gcctggattc
     2761 cggcccgctg tactacctgg acggcgacga cgagcacgaa gacgacgacg gcaacccgct
     2821 ggcgtaccgc aacatcacgc tgggctccaa cgcgggcggc aactccttcc atttccatgc
     2881 cggcaatcag gcgggcgtgg ccaccatcac ctgctcggta catgacccgc gcgccaaccg
     2941 cgaggtcagc gccagcgtcg acatcacggt cggcggcggc tcgggcaccg gcatggccgc
     3001 cagcatccgc ggcgtggccc agcacgccac cctgggcacc cagggcaacc tgaacaacct
     3061 gcgcaccagc acggccatca gcgcctatgt gtgggatgac gccaaccagc ccgtgccgag
     3121 caacggcaaa tccaatgtgc aggccagcat cgtcggcagc accgcgccgg gcgccccggc
     3181 atggggcgcg cgcctgctgg ctggcgccca gcagggcagc acggtccagg tgtcgaccac
     3241 tggcggcgtg ggcttgttct cgctgtccag cggccccaac cgtggcccga tcctgctcga
     3301 actgaaggcc gaccgacgcg acaacgacgt cagcaacggc atccaggacg aagtgcgcca
     3361 cctgctggtg gtgtgggcgg tcgagggtgt ggcggccacc ccgctgagcc tgaaaccggt
     3421 cgaattcgat gtgcccaacg ccacgccgtt cggcgcggtc ctgagcgtca ccggtggcga
     3481 agcgccctac acctggacgg cgctggacgc cctgcccacg ggtctgtcgc tcagcagcgg
     3541 cggtgtgctg caggg
//
LOCUS       DAOQTZ010000185         3500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_30627_length_3500_cov_13.941655, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000185 DAOQTZ010000000
VERSION     DAOQTZ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30627_length_3500_cov_13.941655"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PKN51_13505"
     CDS             <1..1055
                     /locus_tag="PKN51_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase"
                     /protein_id="HNT86161.1"
                     /translation="PSWIMKYYILDLSPHNSMVRYLVGQGHTVYMVSWRNPDESDRNL
                     SMDDYLKQGVLDPMRAARQDAEGAPLHAMGYCLGGTLMAIAVCALARDGGMRGYTDLP
                     APLTLTLLAAQVDFHAPGELGLFIDEGQINLLGHITKGQGYLSGKQMGGSFQFLHSRD
                     LVWTRQMREYLMGERDQGNDLMAWNADTTRLPARMHHEYLVALYLKNALANGRYVAGG
                     RRVSLGDLNMPVFLVGTERDHVSPWRSVYKLHQLTGTEISFLLTSGGHNAGIISPPGH
                     PRRSYRLRTQPKGSQWLSMDEWLATAESFEGSWWPAWQAWLAAHSSTPVKARPIPARH
                     ALYPAPGEYVMQRYAE"
     gene            1174..2484
                     /locus_tag="PKN51_13510"
     CDS             1174..2484
                     /locus_tag="PKN51_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNT86162.1"
                     /translation="MTLFRLSCIAACAALVVACGGSGDSGPRRGELIDPAATLTTLST
                     AQIDGATAASGLQTLTGAAVCDVKVVALNYATVGAKAGEHSNASGVMLLPSGAAGSAC
                     ATNAAPLLAYARGTDVNKSRNLANPQDPETLLLAAFFAAQGYAVVATDYLGYNKSTYG
                     FHPYLHADSEATAVIDSVRAARHAAAALGGKLSGKVMFSGYSQGGHSSMAAQRAAERD
                     YGNEFNVAAGAHLAGPYNLAGAMKVPQAIAGYQFFVPFIITAWQKVYGNIYTSADQVF
                     KPPYAATIDNLLPGPNASYATLVGGGKLPGGTPDQARDALMQPAFLQAMQADDKAPIN
                     IDAVKNTLFDWKPKAPVLLCGGSGDPTVVQAVHQIPMMANFIANDVSNFTSVDVDDAI
                     AATFGPAPTDPTSAAYATYYGNYHGSYEPPFCLAAARGLFNQVR"
     gene            complement(2600..3118)
                     /locus_tag="PKN51_13515"
     CDS             complement(2600..3118)
                     /locus_tag="PKN51_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNT86163.1"
                     /translation="MPEAGAQGEPAAAPALAFWRIATDTPDHTADDLSGQGAERAGGR
                     WNARGTPLVYASSSRALACLETVVHLGGGLALPLNRYLVCIELPPDVWAARTVFDVAV
                     QVGWDALPPGRVSIAWGTAWAQGGASCVAEVPSVIVPEEANLLINPRHPDARRIRGRK
                     LRRWLYDPRSAR"
     gene            complement(3111..>3500)
                     /locus_tag="PKN51_13520"
     CDS             complement(3111..>3500)
                     /locus_tag="PKN51_13520"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNT86164.1"
                     /translation="RAELVKRGLPARAVQEMSAAMGVPREQLMRAVGLARSTVERKLA
                     ARGRLSESESEKLVGLSRLIGQVDAMVRESGAAPAGFDAARWFAAWMAEPVAALGGLR
                     PQQLLDTADGREALATLLLQMRSGVYA"
BASE COUNT          539 a         1347 c         1131 g          483 t
ORIGIN      
        1 tgccgtcgtg gatcatgaag tactacatcc tcgacctgtc gccacacaac tcgatggtgc
       61 gctacctggt cggccagggc cacacggtgt acatggtgtc gtggcgcaac ccggacgaat
      121 ccgaccgcaa cctgtcgatg gacgactacc tgaaacaggg cgtgctcgac cccatgcgcg
      181 cagcccggca ggacgccgaa ggcgcgccgc tgcacgccat gggctactgc ctgggcggca
      241 cgctgatggc catcgccgtg tgcgcgctgg cgcgcgacgg cggcatgcgc ggctacaccg
      301 acctgcccgc gcccctgacg ctgacgctgc tggcggcgca ggtcgacttt catgcgccgg
      361 gcgagctggg cctgttcatc gacgagggcc agatcaacct gctgggccac atcaccaagg
      421 gccagggcta cctgtcgggc aagcagatgg gcggctcgtt ccagttcctg cactcgcgcg
      481 acctggtgtg gacgcgccag atgcgcgaat acctgatggg cgagcgcgac cagggcaacg
      541 acctgatggc ctggaacgcc gacaccacgc gcctgccggc acgcatgcac cacgaatacc
      601 tggtggcgct gtacctgaag aacgccctcg ccaatggccg ctacgtggcg ggcgggcggc
      661 gcgtgtcgct gggcgatctg aacatgccgg tgttcctggt cggcaccgaa cgcgaccacg
      721 tctcgccctg gcgctcggta tacaagctgc accagctcac cggcaccgaa atcagcttcc
      781 tgctgaccag cggcggccac aacgccggca tcatctcgcc gcccggccac ccgcgccgca
      841 gctaccgcct gcggacccag cccaagggca gccagtggct cagcatggac gagtggctgg
      901 ccacggccga gtccttcgaa ggttcctggt ggccggcctg gcaggcctgg ctggccgcgc
      961 attccagcac cccggtcaag gcccggccca tcccagcccg ccacgcgctg tatcccgcgc
     1021 cgggcgaata cgtgatgcag cgctacgccg aatagcgccg ccgcggccgg ccagcggcgc
     1081 cggcatggcc gcgtaattcc ctatggatgt tgcccggcgc tgcgccgacg atgcgggtct
     1141 tgttacgttc cttacccgca agaaaggcat gccatgaccc tgtttcgact gtcctgcatc
     1201 gcggcctgcg ccgccctggt ggtggcctgc ggcggctccg gtgactccgg cccccgccgc
     1261 ggcgagctga tcgatccggc cgccacgctg accacgctga gcaccgccca gatcgacggc
     1321 gccaccgccg ccagcggcct gcagacgctc accggggcgg cggtgtgcga tgtcaaggtg
     1381 gtggccctga actacgccac cgtcggcgcc aaggccggcg agcacagcaa cgcctcgggc
     1441 gtcatgctgc tgcccagcgg cgccgccggc tcggcctgcg ccaccaacgc cgcgccgctg
     1501 ctggcctacg cgcgcggcac cgacgtcaac aagtcgcgca acctggccaa cccgcaggac
     1561 cccgaaaccc tgctgctggc cgccttcttc gccgcccagg gctacgccgt ggtggccacc
     1621 gactacctgg gctacaacaa gtcgacctac ggcttccacc cctacctgca cgccgactcc
     1681 gaggccacgg cggtgatcga ctcggtgcgc gcagcgcgcc atgccgctgc ggcgctgggc
     1741 ggcaagctgt cgggcaaggt gatgttcagc ggctactcgc agggtggcca ttcctccatg
     1801 gcggcgcagc gcgccgccga gcgcgactac ggcaacgaat tcaacgtggc ggctggtgcc
     1861 catctggccg gcccctacaa cctggctggc gccatgaagg tgccgcaggc catcgccggc
     1921 taccagttct tcgtgccctt catcatcact gcctggcaga aggtgtacgg caacatctac
     1981 accagcgccg accaggtgtt caaaccgccc tacgccgcca ccatcgacaa cctgctgccc
     2041 ggcccgaacg ccagctacgc caccctggtg ggcggtggca agctgcctgg cggcacaccc
     2101 gatcaggcgc gtgacgcgct gatgcaaccg gcctttctgc aggccatgca ggccgacgac
     2161 aaggcaccca tcaatatcga cgccgtcaag aacacgctgt tcgactggaa acccaaggcc
     2221 ccggtgctgt tgtgcggcgg ctccggcgac ccgaccgtgg tgcaggcggt gcaccagatc
     2281 ccgatgatgg ccaatttcat cgccaatgac gtcagcaact tcacctcggt cgatgtggac
     2341 gacgccatcg ctgccacctt cggtccggcg cccaccgacc cgacatcggc cgcctacgcc
     2401 acctactacg gcaactacca cggcagctac gaaccgccgt tctgcctggc ggccgcgcgc
     2461 ggtctgttca accaggtgcg ctgaacccgc cagcctcggg cggctgcgct ggccttgacc
     2521 cgccggcgca gcccaccacg tgcccgccca cgccacctgc atcggcctgc gggccggcct
     2581 ggcccgggcc cggcgcccgt cagcgcgcgc tgcgcgggtc gtacagccag cggcgcagct
     2641 tgcgcccgcg gatgcgccgg gcgtcggggt ggcgcgggtt gatcagcaga ttggcttctt
     2701 ccggcacgat gaccgagggc acttcggcca cgcagctggc cccgccctgc gcccacgccg
     2761 tgccccaggc gatgctgacg cggcccggcg gcagcgcgtc ccaacccact tgcacggcaa
     2821 cgtcgaacac cgtgcgcgcc gcccacacat cgggcggcag ctcgatgcac accagatagc
     2881 ggttgagcgg caacgccagg ccgccgccca ggtgcaccac ggtctccaga caggccagcg
     2941 cacgcgagct gctggcgtac accagcggcg tgccgcgcgc attccagcgc ccgccggcgc
     3001 gctcggcacc ctggccgctc aggtcgtcgg cggtgtggtc gggcgtgtcg gtggcgatgc
     3061 gccagaaggc cagcgccggc gccgccgcag gctcgccttg cgcgccggcc tcaggcatac
     3121 acgcccgatc gcatttgcag cagcagcgtg gccagcgcct cgcggccgtc ggcggtgtcc
     3181 agcaactgct gcgggcgcag gccgcccagc gccgccaccg gctcggccat ccaggcggcg
     3241 aaccagcgcg cggcatcaaa gccggcgggc gccgcgcccg attcgcgcac catggcatcc
     3301 acctgcccga tcaggcgcga caggccaacc agcttttcgc tctcgctctc ggacagccgg
     3361 ccgcgcgccg ccagcttgcg ctccaccgtc gagcgcgcca gccccacggc gcgcatcagc
     3421 tgttcgcgcg gcacacccat ggcggcgctc atctcctgca ccgcgcgcgc cggcaggccg
     3481 cgcttgacca gctcggcccg
//
LOCUS       DAOQTZ010000186         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_31084_length_3463_cov_11.634683, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000186 DAOQTZ010000000
VERSION     DAOQTZ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31084_length_3463_cov_11.634683"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1961)
                     /gene="dnaG"
                     /locus_tag="PKN51_13525"
     CDS             complement(147..1961)
                     /gene="dnaG"
                     /locus_tag="PKN51_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNT86165.1"
                     /translation="MAIPQHFLQELLARADIVEIVGRYVPLKKTGANFSGLCPFHGEK
                     TPSFTVSPSKQFYHCFGCGKNGNAIGFLMDHTGAGFVETVHELAQQYGLQVPEDERSP
                     AERARAAEQTEQRASLTDVLARAADAWRKQLKASPRAIDYLKRRGLSGQVARQFGLGY
                     APEGWRGLASVFPDYADPLLVESGLVIVSEDDEGKRYDRFRDRIMFPIRSVKGEVIGF
                     GGRVLGDDKPKYLNSPETPVFHKGRELYGLFEARTALREQGYALVVEGYMDVVALAQQ
                     GLGNAVATLGTACTPEHVHKLFRFTDHVVFSFDGDAAGRRAAHKALEAALPLATDTRS
                     VKFLFLPPEHDPDSFVRAEGVEAFAQAVRDAVPLSRFLLDAAAEDCDLQTAEGRSRFA
                     ARARPLWQLLPTGALAAQLLGDIAAQVQIGTHELEQLWGLRRTPPARSENNSYSRSPH
                     KAREPKTPARAVAVRHAPRRAPSPRVSRALQIVLTEPSAWDRLTQSDQGLLCDLPAPL
                     GALFTWLAGQHLEHGPQPWSALREALRGHGHEAFAVELLERLPPDIESDAEELSRILT
                     LEHDARYAAERQRLAAAAAAGDTAAYARLREMPVTRPR"
     gene            2130..>3463
                     /locus_tag="PKN51_13530"
     CDS             2130..>3463
                     /locus_tag="PKN51_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT86166.1"
                     /translation="MPSIFDTDLPRNPANHAALTPLAFIQRTAELYPGRLAIVHGELR
                     QSWGQTYARCRQLASSLQRHGIGRNDTVAVMLPNTPPMVEAHFGVPMAGAVLNTLNTR
                     LDPEAVAFMLDHGEAKAVIVDPEFAGIMKKALALRQSKAPLLVIDVEDALYGPGQPIG
                     SIGYEAFVAQGDAGFEWDYPGDEWDAIALNYTSGTTGNPKGVVYHHRGAAINAISNIL
                     EWDMPKHAVYLWTLPMFHCNGWCFPWTVAARAGVNVCLRRVEARAIFDAIRQHGVTHY
                     CGAPIVHGLLVNAPDELKAGVPSGVKCMVAGAAPPASMIEGMERLGFDLTHVYGLTEV
                     YGPATVCAKHEAWEQLAIGERAALNARQGVRYHLQRDARVLDPETLQPVPMDGETMGE
                     IMFRGNIAMKGYLKNPGATEEAFRGGWFHSGDLAVQYPDGYIKIKDRSKDII"
BASE COUNT          536 a         1158 c         1227 g          542 t
ORIGIN      
        1 aggacacccc aaaggggaca tctctatttg ggctgaaagg ggacatttct attttttggg
       61 ttgacactca tttcaaggac ccatttcaag gtccccaagg gattggcgac gccgccgcgc
      121 ccgccgtcga tggggagtgg ttcggttcag cgcgggcggg tgaccggcat ttcacgcaac
      181 cgggcgtagg cggcggtgtc tccggcagcc gcggcggcgg ccaggcgctg gcgctcggcg
      241 gcgtagcggg cgtcgtgctc cagcgtcagg atgcgcgaca gttcttcggc gtcgctttcg
      301 atgtcgggcg gcaggcgctc cagcagctcg acggcaaagg cctcgtggcc gtggccgcgc
      361 agggcttcgc gcagcgcgct ccacggctgc ggaccgtgct ccaggtgctg gccggccagc
      421 caggtgaaca gcgcgcccag gggcgccggc aggtcgcaca gcaggccctg atcgctttgc
      481 gtgaggcgat cccaggcgct cggctcggtc agcacgatct gcagggcacg gctgacgcgc
      541 ggcgagggtg cgcgccgcgg cgcgtggcgg acggccacgg ctcttgcggg tgtttttggc
      601 tctcttgcct tatggggtga gcgcgaatag ctattgtttt cagagcgtgc cgggggcgtt
      661 cgacgcaagc cccatagttg ctccagctcg tgcgtgccga tctgcacctg cgcggcgatg
      721 tcgcccagca gctgtgccgc cagcgccccc gtgggcagca actgccacag tggccgggct
      781 cgggccgcga agcgcgagcg gccttcggcg gtctgcaggt cgcagtcctc ggccgcggcg
      841 tccagcagga agcgcgacag cggcacggcg tcacgcaccg cctgggcgaa ggcctcaacc
      901 ccctcggcac gcacgaagct gtcggggtcg tgctcgggcg gcagaaacag gaacttcacg
      961 ctgcgggtgt cggtggccag gggcagggcg gcttccagcg ccttgtgggc cgcgcgtcgg
     1021 ccggcggcgt cgccgtcgaa gctgaacacg acgtggtcgg tgaagcgaaa cagcttgtgc
     1081 acgtgctcgg gcgtgcaggc tgtgcccagg gtggcgacgg cgtttcccag gccctgctgc
     1141 gccagcgcca ccacgtccat gtagccttcc accaccagcg cgtagccctg ctcgcgcagc
     1201 gcggtgcgcg cttcgaacag gccgtacaac tcgcggccct tgtgaaacac cggggtttcg
     1261 ggagagttca ggtatttggg cttatcgtcg cccagcacgc ggccaccgaa gccgatcact
     1321 tcgcccttga ccgagcggat ggggaacatg atgcgatcgc ggaaacggtc gtagcgcttg
     1381 ccttcgtcgt cctccgacac gatgaccagc ccgctttcca ccagcagggg gtcggcgtag
     1441 tccgggaaca cgctggccag gccgcgccag ccctcgggcg cgtagcccag gccgaactgg
     1501 cgcgccacct ggcccgacag gccgcgccgc ttgaggtagt cgatggctcg tggcgaggcc
     1561 ttgagctgct tgcgccaggc gtcggcggcg cgggccagca cgtcggtcag gctggcgcgc
     1621 tgctcggtct gctcggcggc gcgggcgcgc tcggccgggc tgcgttcgtc ctcgggcacc
     1681 tgcaggccgt attgctgggc cagttcgtgc accgtctcga cgaagccggc gccggtgtgg
     1741 tccatcagaa agccgatggc gttgccgttc ttgccgcagc cgaagcagtg gtagaactgc
     1801 ttggacgggc tgacggtgaa cgacggcgtc ttttcgccat ggaacggaca caggccggaa
     1861 aaattggcgc cggtcttctt gagcggcacg tagcggccga cgatctcgac gatgtcggcg
     1921 cgcgccagga gctcctggag aaagtgctgc ggaatggcca cgaggctatt ttgcccgggc
     1981 catctcgggg acgtcaggcg atcgagccga tcgacgtgcc gcgcgctgcg gggttccggg
     2041 caggcacggg accggcgcca gctgcccgtc aggccgctga ctatgatcgg gcgtcccgcc
     2101 cccgcgcgtt ccctttccag gagacaggca tgcccagcat cttcgacacc gatctgcctc
     2161 gcaacccggc caaccatgct gcactgacgc cgctggcctt catccagcgc acggccgagc
     2221 tgtatccggg ccgcctggcc atcgtgcatg gcgagctgcg ccagagctgg ggccagacct
     2281 atgcgcgctg tcgccagctg gccagcagcc tgcagaggca cggcatcggc aggaacgaca
     2341 ccgtggccgt gatgctgccc aacaccccgc ccatggtcga ggcgcatttc ggcgtgccga
     2401 tggccggcgc cgtgctcaac acgctcaaca cccggctcga ccccgaggcg gtggccttca
     2461 tgctggacca cggcgaggcc aaggcggtca tcgtcgaccc cgagttcgcg ggcatcatga
     2521 agaaggcgct ggcgctgcgc cagtcgaagg cgccgctgct ggtgatcgac gtcgaggacg
     2581 cgctgtacgg cccggggcag ccgatcggca gcatcggcta cgaggccttc gtggcgcagg
     2641 gcgacgccgg cttcgagtgg gactatccgg gcgacgagtg ggacgccatc gcgctcaact
     2701 acaccagcgg caccaccggc aaccccaagg gcgtggtcta ccaccaccgc ggcgcggcca
     2761 tcaatgccat cagcaacatc ctcgaatggg acatgcccaa gcacgcggtc tatctgtgga
     2821 cgctgccgat gttccactgc aacggctggt gctttccgtg gacggtggcg gcgcgcgccg
     2881 gtgtgaacgt gtgcctgcgc cgggtcgagg cgcgggcgat cttcgacgcc atccgccagc
     2941 atggcgtgac gcactactgc ggcgcgccca tcgtgcacgg cctgctggtc aacgcaccgg
     3001 acgagctgaa ggccggtgtg cccagcggcg tcaaatgcat ggtggcgggc gcggcgccgc
     3061 cggcctcgat gatcgagggc atggagcgcc tggggttcga tctgacccac gtctacggcc
     3121 tgaccgaggt ttatggcccg gccaccgtgt gcgccaagca cgaggcgtgg gagcaactgg
     3181 ctattggcga gcgcgcggcg ctgaacgcgc gccagggcgt gcgctaccac ctgcagcgcg
     3241 acgcgcgcgt gctcgacccc gagaccctgc agccggtgcc gatggacggc gaaaccatgg
     3301 gcgaaatcat gttccgcggc aacatcgcca tgaagggcta tctgaagaac cccggcgcca
     3361 ccgaggaagc cttccgtggc ggctggttcc acagcggcga cctggccgtg cagtaccccg
     3421 acggctacat caagatcaag gaccgcagca aggacatcat cat
//
LOCUS       DAOQTZ010000187         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_31963_length_3395_cov_13.812874, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000187 DAOQTZ010000000
VERSION     DAOQTZ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31963_length_3395_cov_13.812874"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKN51_13535"
     CDS             <1..222
                     /locus_tag="PKN51_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HNT86167.1"
                     /translation="WVKAKVRRVVHQLDIHTLAEQQAEQLAPNAIGHVELLLQEPLPA
                     APFAQARVLGSLILVDTASHRTAGAVLVR"
     gene            complement(245..1012)
                     /locus_tag="PKN51_13540"
     CDS             complement(245..1012)
                     /locus_tag="PKN51_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNT86168.1"
                     /translation="MNPLDAPIADPASVDAAIESRFSVRAFLPQPVPRPLIEDILRVA
                     SRAPSGTNAQPWKVYVLQGAARDEVVRQVSQAREAIRDRPELANDYREAYDYYPRQWV
                     EPYLSRRRHNGWSLYGLLGIERGDKDRMHAQHQRNFSFFDAPVGLMFTLDRVMGQGSL
                     VDYGMFLQNIMLAARARGLHSCAQGAWNPFSRIVLPLIGASEQEMLVCGMALGYADPS
                     APVNRFWTPREPVEVFTRWIEQPADTPVPEGEAGSPD"
     gene            complement(1009..1446)
                     /locus_tag="PKN51_13545"
     CDS             complement(1009..1446)
                     /locus_tag="PKN51_13545"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT86169.1"
                     /translation="MSTARPQPDPRDSYRAFRTITTRWMDNDQYGHVNNVVYYSWFDT
                     AVNAHLIEQGVLDTAAGATIGLVVETQCHYFAPLSFPQTIEAGIRVAHMGSSSVRYEV
                     GLFAQGAPLTAARGHFVHVYVDRATQRPVPLPEPLRQVLQALR"
     gene            complement(1443..2378)
                     /locus_tag="PKN51_13550"
     CDS             complement(1443..2378)
                     /locus_tag="PKN51_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNT86170.1"
                     /translation="MRTFYSTSQGLALPPGHRFPMGKYAALRDRLSAECPGLRLAQAP
                     PASDAELALAHAPAYVAAVVQGRLDARAQREIGFPWSEAMVERSRRSVGASIEATRVA
                     QHEGVAANLAGGTHHASAEAGSGYCVFNDIAVAARLLQAERTGGGQPARLTRVAVIDL
                     DVHQGNGTAGIFAGDASVFTLSLHGEKNFPFRKVAGDLDVGLPDGTGDQVYLDALDLA
                     LVELDSRFRPDAVFYLAGADPHEGDRLGRLKLSYDGLLARDRRVFDWAWTRRLPLTLC
                     MGGGYGHDIATTVQVQVNTFGVALQYWQRWQNHRP"
     gene            2560..3105
                     /locus_tag="PKN51_13555"
     CDS             2560..3105
                     /locus_tag="PKN51_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNT86171.1"
                     /translation="MLTPEQLVASQKANMETLYGLTTKAFEGVEKLVELNLQATKAAL
                     AESQAHANAMLSVKDAQELIALQAGLFQPLAEKAAAYSRHLYDIASGTGAEFSKAFES
                     KVAEGQAQFASLVDNAAKNAPAGSETAVAMMKSAVAAANNAFESVQKAVKQATDVAEA
                     NFNAVATSATNVAKTATAKKR"
BASE COUNT          562 a         1226 c         1111 g          496 t
ORIGIN      
        1 tgggtcaagg ccaaggtgcg ccgcgtggtg caccagctcg acatccacac cctggccgag
       61 cagcaggccg agcagcttgc gcccaacgcc atcggccatg tggagctgct gctgcaggag
      121 cccctgcccg cggcaccgtt tgcacaggcg cgcgtgctgg gctcgctcat cctggtcgac
      181 acggccagcc acaggacggc gggcgcggtg ctggtgcgct gaccgcagcc gcccggccga
      241 cgcgtcagtc gggcgagccg gcctcgccct cgggcacagg cgtgtcggcc ggctgctcga
      301 tccagcgcgt gaacacctcc accggctcgc gcggcgtcca gaagcggttg accggtgccg
      361 acgggtcggc atagcccagc gccatgccgc acaccagcat ctcctgttcc gaagcgccga
      421 tcagcggcag cacgatgcgt gaaaacgggt tccacgcgcc ctgggcgcag ctgtgcaggc
      481 cgcgcgcacg cgccgccagc atgatgtttt gcaggaacat gccgtagtcg accaggctgc
      541 cctgccccat cacccgatcg agcgtgaaca tcaggcccac cggcgcgtcg aagaagctga
      601 aattgcgctg gtgctgggca tgcatgcggt ccttgtcgcc gcgctcgatg cccagcaggc
      661 cgtacagcga ccagccgttg tggcgccgcc ggctcagata gggctcgacc cactggcgcg
      721 ggtagtagtc gtaggcctca cggtagtcgt tggccagctc ggggcggtcg cggatcgcct
      781 cgcgcgcctg gctcacctgg cgcaccacct cgtcgcgtgc cgccccctgc agcacgtaca
      841 ccttccacgg ctgggcgttg gtgccgctgg gcgcgcggct ggccacgcgc aggatgtcct
      901 cgatcagcgg ccgcggcacc ggctgcggca gaaaggcgcg caccgaaaag cggctttcga
      961 tcgccgcgtc cacgctggcc ggatcggcga tgggggcgtc gagcgggttc accgcagggc
     1021 ctgcagcact tgccgcagcg gctcgggcag cggcaccggg cgctgcgtgg cacggtccac
     1081 gtagacatgc acgaagtgcc cgcgcgcggc cgtcagcggt gcgccctggg cgaacaggcc
     1141 gacctcgtag cgcacgctgg agctgcccat gtgtgccacg cgaatgccag cctcgatggt
     1201 ctgcggaaac gacagcggcg cgaaatagtg gcactgcgtc tccaccacca ggccgatggt
     1261 ggcgccagcg gcggtgtcca gcacgccctg ctcgatcagg tgggcgttga cggcggtgtc
     1321 gaaccacgag tagtacacga cgttgttgac gtggccgtac tggtcgttgt ccatccagcg
     1381 cgtggtgatg gtgcggaagg cgcggtagct gtcgcgcggg tcgggttgcg ggcgtgcggt
     1441 gctcatggcc ggtgattctg ccagcgctgc cagtactgca aggccacgcc gaaggtgttg
     1501 acctgcacct gcaccgtggt ggcgatgtcg tggccgtagc caccgcccat gcacagcgtc
     1561 aggggcaggc gccgcgtcca ggcccagtcg aacacccggc gatcgcgcgc cagcaggccg
     1621 tcatagctga gcttcaggcg ccccagacgg tcgccctcgt gcgggtcggc gccggccagg
     1681 tagaacacgg cgtcggggcg aaagcggctg tccagctcga ccagcgccag gtccagcgcg
     1741 tccagataga cctggtcacc cgtgccgtcc ggcaggccca cgtccagatc gcccgccacc
     1801 ttgcgaaacg gaaagttctt ctcgccgtgc agcgacaacg tgaacacgct ggcgtcgccg
     1861 gcgaagatgc cggccgtgcc attgccctgg tgcacgtcca gatcgatcac ggcgacgcgc
     1921 gtcaggcgcg ccggctggcc gccacccgtg cgctcggcct gcagcaggcg cgcggccacg
     1981 gcaatgtcgt tgaacacgca gtagccgctg ccggcttccg cgctggcgtg gtgcgtgccg
     2041 ccggccaggt tggccgccac cccctcgtgc tgggccacgc gcgtggcctc gatgctggcg
     2101 ccgaccgaac ggcgcgagcg ctcgaccatc gcctcgctcc acggaaagcc gatctcgcgc
     2161 tgcgcccgcg catccagccg gccctgcacc accgcggcca cgtaggccgg cgcatgggcc
     2221 agcgccagtt cggcgtcgct ggccggcggc gcctgcgcca gccgcagccc ggggcattcg
     2281 gcgctcagcc gatcgcgcag ggcggcgtac ttgcccatcg gaaagcgatg cccgggcggc
     2341 agcgccaggc cctgcgaggt cgaatagaag gtcctcatgc cacgccgccg ccaacggcgc
     2401 ccgcgccacc ggttagaccg ccgcatctaa aaatgctgca acgcaccaaa aacgcttgca
     2461 ttcctgcccc cgggccctaa aattccatcc catgctgcac cgcaacataa caagcagcaa
     2521 cccgttattt tttgatccac cattgtctag gagaacccta tgctgacccc cgaacaactc
     2581 gtcgcttccc agaaggccaa catggaaacc ctgtacggcc tgaccaccaa ggccttcgaa
     2641 ggcgttgaaa agctggtcga gctgaacctg caagccacca aggccgccct ggccgagtcg
     2701 caggcccacg ccaacgccat gctgtcggtc aaggacgccc aggagctgat cgccctgcag
     2761 gctggtctgt tccagccgct ggccgagaaa gccgccgcct acagccgtca cctgtacgac
     2821 atcgcttcgg gcaccggcgc cgagttcagc aaggcgttcg agtccaaagt ggccgaaggc
     2881 caggcccagt tcgcctcgct ggtggacaac gccgccaaga acgcccccgc cggctccgag
     2941 accgccgtcg ccatgatgaa gagcgccgtg gccgccgcca acaacgcctt cgagtcggtg
     3001 cagaaagccg tcaagcaagc gaccgacgtg gccgaagcca acttcaacgc cgtcgccacc
     3061 agcgccacca acgtcgccaa gaccgcgacc gccaagaagc gctgatcgcc gaccggccag
     3121 cccgcccacg gtttcacccg acccgaggcg cactggccac ccggtgcttt gccgcccgcc
     3181 ctgcgtggtc ggcaaagcca ccagagccaa accggtgccc ggcgcgggtt tggctctggt
     3241 gccgagtccg gcacctcatg cttgtctcct cggatccttt cgacacccat ccggttcagg
     3301 gatcccttca agggctgatc gcaagatcag cccttttttt gtcgcgttaa tgcgacacgt
     3361 atgccgcatg ttggagacgc aagtcacgct ggagc
//
LOCUS       DAOQTZ010000188         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_33823_length_3262_cov_14.580293, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000188 DAOQTZ010000000
VERSION     DAOQTZ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33823_length_3262_cov_14.580293"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /gene="ureC"
                     /locus_tag="PKN51_13560"
     CDS             <1..300
                     /gene="ureC"
                     /locus_tag="PKN51_13560"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656514.1"
                     /note="ureases catalyze the hydrolysis of urea into
                     ammonia and carbon dioxide; in Helicobacter pylori the
                     ammonia released plays a key role in bacterial survival by
                     neutralizing acids when colonizing the gastric mucosa; the
                     holoenzyme is composed of 3 ureC (alpha) and 3 ureAB
                     (gamma/beta) subunits; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNT86172.1"
                     /translation="QPVHYRPMFGAFAGSKARSSLTFVSQAGLAAGIGERFGLHKPLA
                     AVKGIRGVQKRHMIHNDLAPHMEVDAQTYAVRANGELLVCEPATSLPMAQRYFLF"
     gene            complement(285..806)
                     /locus_tag="PKN51_13565"
     CDS             complement(285..806)
                     /locus_tag="PKN51_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNT86173.1"
                     /translation="MSISRDFSSTRTPAPQWQPCGAAHVAVYGTLRAGGINDIARLGS
                     GVVCVGTTRLSGRLHDLGWYPGLRLGGAQQVLAEVYAINPAIEQAMDRIEGLWPEDLG
                     EYAKRLLTLPVALAGGGTQTLTVLVYEALPATVAQAPVIEADDWLAWATQQGKAVRHT
                     DEAFALNAAQNRK"
     gene            896..1414
                     /gene="ureE"
                     /locus_tag="PKN51_13570"
                     /pseudo
     CDS             896..1414
                     /gene="ureE"
                     /locus_tag="PKN51_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
     gene            1569..2228
                     /locus_tag="PKN51_13575"
     CDS             1569..2228
                     /locus_tag="PKN51_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNT86174.1"
                     /translation="MWLASPALPIGGFSYSEGLEAAVQAAHVTTEDEAARWLVQQLHL
                     TQARGDMAAVAQALAAWRHGDLARVQQLNDWVLATRETSELRLQTEQMGRSLTEWLKN
                     RHTGHAAAMPQVQWLAARNPSYPMAFALAAHLTAAQTRDALLAYAFGWAENMVQAAIK
                     AVPLGQNAGQRILARLTGEIPAAVAHAQALPDAARQAFSPMLAILSARHEHQYSRLFR
                     S"
     gene            2225..2887
                     /gene="ureG"
                     /locus_tag="PKN51_13580"
     CDS             2225..2887
                     /gene="ureG"
                     /locus_tag="PKN51_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HNT86175.1"
                     /translation="MTSSALHHIPHRTRKLPPLRVGIGGPVGSGKTTLLEMLCKAMRD
                     KWDLVAITNDIYTKEDQRLLTISGALPPERIMGVETGGCPHTAIREDASINLEAIDRM
                     LGQFPDADIVFIESGGDNLAATFSPELSDLTIYVIDVAAGEKIPRKGGPGITKSDLFV
                     INKTDLAPHVGANLDVMRADTARMRTTPKGLKPFVMTNLKTLQGLDEVVAFIERQGLL
                     VA"
     gene            2925..3170
                     /locus_tag="PKN51_13585"
     CDS             2925..3170
                     /locus_tag="PKN51_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86176.1"
                     /translation="MSDLEMTDQSQQPHLTRPAYRLAGAALGVLLLACGLYVAFATDT
                     DALSWLGVALLLAFGGNLLVSAWRGRASWISRIGPLP"
BASE COUNT          539 a         1197 c         1041 g          485 t
ORIGIN      
        1 cagccggtgc actaccggcc gatgttcggc gcgtttgccg gcagcaaggc gcgcagctcg
       61 ctcacctttg tctcgcaggc cgggctggcg gcgggcatcg gcgagcgctt cggcctgcac
      121 aagccgctgg cggcggtcaa gggcatacgc ggcgtgcaaa agcgccacat gatccacaac
      181 gacctggcgc cgcacatgga agtcgatgcc cagacctacg ccgtgcgcgc caatggcgag
      241 ctgctggtgt gcgaacccgc caccagcctg cccatggcgc agcgctactt cctgttctga
      301 gccgcgttca gcgcaaacgc ctcgtccgta tgccgcaccg ccttgccctg ctgcgtggcc
      361 caggccagcc agtcatccgc ctcgatcacc ggcgcctgcg ccacggtggc gggcagggct
      421 tcgtagacca gcaccgtcag ggtctgcgtg ccgccgcccg ccagcgcgac gggcagggtc
      481 agcaggcgct tggcgtattc gcccagatct tcgggccaca ggccttcgat gcggtccatg
      541 gcctgctcga tcgcggggtt gatggcatag acctcggcca gcacctgctg cgcaccgccc
      601 agcctcaggc ccggatacca gcccagatca tgcaggcggc ccgacagccg ggttgtgccc
      661 acgcagacca cgccgctgcc cagccgcgca atgtcattga tgccgcctgc gcgcagcgtg
      721 ccgtagaccg ccacgtgcgc ggcgccgcag ggctgccact ggggcgcggg cgtccgtgtc
      781 gaagaaaaat cacgcgagat tgacatgcct gtcatgctat cgggttgcac aggccaaggg
      841 tgatgcacaa agcgcgccaa ccggccctgg cagccttctc catccaacgt acaccatgct
      901 gactgtcaac aaacttcttc cgcgcggcca gggcctcgcc ggccccctgc tccagcgcgc
      961 cgccagcgtc gagctggact gggatgtgcg ccagaaatcc cgctttgcag ccaccgacag
     1021 ccagggccgc gcgctggcgg tgtttctgcc gcacggtcag gccgtgcgcg gtggcgacgt
     1081 gctggtggcc gaagacggct cgctgatccg cgtgcaggca gcaaagcaga aggtgctgca
     1141 catcacggcc tgcgccgagc acggcagcac cttcgacctg atgcgcgccg cctaccacct
     1201 gggcaaccgc catgtaccga tcgagctgca gcccgaccac ctgaagatcg agcccgacca
     1261 tgtgctggcc gacatgctgc gcgccatgca catgaccgtg gtcgaggccg agttgccgtt
     1321 cgaacccgaa ggcggtgcct acggtgggca tgtgacccac gacgggcaca gccaccatca
     1381 ccacggccat gggcatcacc atgagcacgg ccactgaaag cgcggcgggc gcggcccgcc
     1441 gcgcggccgc tccgaaggcg tccccgcccc cgacttgtcc ggtgaccctt ccccggactg
     1501 aagcagccga cttggcgccg caggaaagca cagaggttcc cgtagccccc gccacgctgc
     1561 tgcagctgat gtggctggcg tcgcccgcgc tgcccatcgg cgggttttcg tattctgaag
     1621 ggctggaagc ggccgttcag gccgcgcatg tcacgaccga ggacgaagcc gcgcgctggc
     1681 tggtgcagca actgcacctg acccaggccc gcggagacat ggccgccgtc gcccaggccc
     1741 tggccgcctg gcgccatggt gacctggccc gcgtgcagca gctcaacgac tgggtgctgg
     1801 ccacgcgcga gaccagcgag ctgcgcctgc agaccgagca gatgggccgc tcgctgaccg
     1861 aatggctgaa aaaccgccat accggccatg ctgccgcgat gccccaggtt cagtggctgg
     1921 ccgcccggaa ccccagctac cccatggcgt ttgccctggc cgcgcacctg accgcggccc
     1981 agacccgcga cgccctgctg gcctatgcct tcggctgggc cgaaaacatg gtgcaggcgg
     2041 ccatcaaggc cgtgccgctg gggcagaacg caggccagcg catcctggcc cggctgaccg
     2101 gtgaaattcc cgccgccgtg gcgcacgccc aggctctgcc ggatgccgcg cgccaggcct
     2161 tctcgcccat gctggccatc ctctcggccc ggcacgagca ccagtactca cgccttttca
     2221 gatcatgacc tcgtctgcgt tgcaccacat cccccaccgc accaggaagc tgcccccgct
     2281 gcgggtgggc atcggcgggc ccgtcggctc gggcaagacc accctcctgg agatgctgtg
     2341 caaggccatg cgcgacaagt gggacctggt ggccatcacc aacgacatct acaccaagga
     2401 agaccagcgc ctgctgacca tcagcggcgc gctgccgccc gagcgcatca tgggcgtgga
     2461 aaccggcggc tgcccgcaca cggccatccg cgaggacgcg tcgatcaacc tggaagccat
     2521 cgaccgcatg ctgggtcagt tccccgatgc cgacatcgtc ttcatcgaat cgggcggcga
     2581 caacctggcc gccaccttca gccccgagct gagcgatctg acgatctacg tgatcgatgt
     2641 cgccgcgggc gaaaaaatcc cccgcaaggg cggccccggc atcaccaaga gcgacctgtt
     2701 cgtcatcaac aagaccgacc tggccccgca tgtcggtgcc aacctggatg tgatgcgtgc
     2761 cgacaccgcc cgcatgcgca ccacgcccaa ggggctcaag ccctttgtca tgaccaacct
     2821 caagaccctg caggggctgg acgaggtggt ggccttcatc gagcgacagg gcttgctggt
     2881 ggcatgagcc cagttcctga ccatctacac acaccacgag tgcgatgagc gatctcgaaa
     2941 tgaccgacca gtcccagcaa ccccacctca cgcgtccggc ctaccggctg gccggcgccg
     3001 cgcttggcgt gctgttgctg gcctgcgggc tctacgtcgc gttcgccacc gataccgatg
     3061 cgctttcgtg gcttggggtg gcgttgctgc tggcgttcgg cggcaacctg ctggtgtcgg
     3121 cttggcgcgg tcgggcgtcc tggatcagcc ggatcgggcc cctgccctga ccgccggcgc
     3181 ccggctgccg gccggcggca cgctaagcta tgcggcggcg cagccagcgc cggattcgtg
     3241 aacgcccccg ccatgcagat ca
//
LOCUS       DAOQTZ010000189         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_38308_length_3003_cov_12.529172, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000189 DAOQTZ010000000
VERSION     DAOQTZ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38308_length_3003_cov_12.529172"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKN51_13590"
     CDS             complement(<1..561)
                     /locus_tag="PKN51_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT86177.1"
                     /translation="MSAPTAPLPVRAVLVTGAARRLGREISLQFARAGWDVLAHYRHA
                     EAEAQALQADVERLGRRCHLLRADLAQPEAACGLMRQASTLHGAPPHCIVNNASIFEA
                     DDARSASAAGLMQHFATNCATPLLLANALAGLLAAGGQPSGGRHSVVHVLDQKVHNLN
                     PDYFSYTVSKLALAQSVALQAQALAPW"
     gene            625..1815
                     /locus_tag="PKN51_13595"
     CDS             625..1815
                     /locus_tag="PKN51_13595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNT86178.1"
                     /translation="MPTVPADVTATASPAPDALPALIAREIRAAGGWLPFERFMHLAL
                     YAPGLGYYARAARQFGQLPRSGSDFVTAPELSPLFARALAVQLADSLAATGTDEVWEF
                     GAGSGALAAGLIEALDALGQPLARYTIVDVSGALRARQRQRLAAHGARVAWADALPPV
                     LRGVVVGNEVLDAMPVTLLARRGGRWFERGVRIGESIEALAQDGQAPAAIKTEAIGLT
                     WEDRPTALRPPLEPAGTHDLVTEIHPQAQAFVATVAEALRRGGAGAAWFIDYGFPEAE
                     YYHPQRSEGTLVCHRAHRVDDQPLADVGDKDISAHVNFTGVALAAQDAGLEVLGYTSQ
                     GRFLLNCGLAEALAAADLPMRANAMKLLDEHEMGELFKVIGLATPGHPPARGFAQGDR
                     THRL"
     gene            1821..2015
                     /locus_tag="PKN51_13600"
     CDS             1821..2015
                     /locus_tag="PKN51_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNT86179.1"
                     /translation="MIRWLIVVFLALVLISGLTPLLRRLGIGRLPGDLHFRLFGREWF
                     IPLTSTLLLSALASLIARWL"
     gene            complement(2033..>3003)
                     /gene="ribD"
                     /locus_tag="PKN51_13605"
     CDS             complement(2033..>3003)
                     /gene="ribD"
                     /locus_tag="PKN51_13605"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNT86180.1"
                     /translation="PVGQAHAEVMALRDAAARGHRTQGATAYVTLEPCAHHGRTGPCC
                     DALVAAGVRRVVAALPDPNPRVGGQGLQRLRDAGVEVALGPPEVAAAARELNIGFFTR
                     MERGTPWVRLKVASSLDGRTALPDGRSQWITSPAARADAQAWRARACAVLTGIGTVLD
                     DDPLLNVRLPDSPRQPALVVLDSQLRTPAQARLWQVAGRRVCIATAVTDAARQQPLQA
                     RGAQVLALPGDDGRVDLPALLAHLARQEVNELHVEAGHRLNGALLRAGLVDELLLYQA
                     PRLLGDGVGIADLGPLAGLDQALAFDWHAIERVGPDLRLLARAPGR"
BASE COUNT          369 a         1095 c         1144 g          395 t
ORIGIN      
        1 ccagggcgcc agcgcctgcg cctgcagggc cacgctctgc gccagcgcca gcttgctgac
       61 ggtgtaggaa aaatagtccg ggttcaggtt gtgcaccttc tggtccagca cgtgcaccac
      121 actgtgccgc cccccgctgg gctggccgcc tgcggccagc aggccggcca gtgcgttggc
      181 cagcagcagg ggcgtggcgc agttggtggc aaagtgctgc atcaggcccg ccgcgcttgc
      241 cgatcgggcg tcgtctgctt caaaaatgga agcattgttg acgatgcagt gcggcggcgc
      301 gccgtgcagc gtggaggcct gccgcatcag cccgcaggcc gcctcgggct gggccaggtc
      361 ggcccgcagc aggtggcagc gccggcccag gcgctcgacg tcggcctgca gcgcctgcgc
      421 ctcggcctcg gcatggcggt aatgcgccag cacgtcccag ccggcgcgcg cgaactgcag
      481 gctgatctcg cgcccgagcc ggcgcgcggc gccggtgacc agcacggcac ggacgggcag
      541 cggggcggtg ggcgcggaca tggggcggca ttgtcgggca aaaggcgcgg cggcacgcgc
      601 cggcgcgctg cggcatggcc cacaatgccg accgtgcctg ccgatgtcac tgccactgcc
      661 tcacctgcgc ccgacgcgct gccggcgctg atcgcgcgcg aaatccgcgc cgccggcggc
      721 tggttgccgt tcgagcgttt catgcacctg gcgctgtacg cgccggggct gggctactac
      781 gcccgggcgg cgcgccagtt tggccagctg ccgcgcagtg gcagcgactt cgtcaccgcg
      841 cccgagctgt cgccgctgtt cgcccgcgcg ctggcggtgc agctggccga cagcctggcg
      901 gccaccggca ccgatgaagt gtgggagttc ggcgccggct cgggcgcgct ggcggccggg
      961 ctgatcgagg cgttggacgc gctgggccag ccgctggcgc gctacaccat cgtcgacgtc
     1021 tcgggcgcgc tgcgggcgcg ccagcgccag cgcctggccg cccatggcgc gcgcgtggcc
     1081 tgggccgacg cgctgccgcc ggtgctgcgc ggcgtggtgg tgggcaacga agtgctggac
     1141 gccatgccgg tgacgctgct ggcgcggcgc ggcgggcggt ggttcgagcg cggggttcgc
     1201 attggtgaat ccattgaggc cctggcgcag gatgggcaag cgccggcagc tatcaaaaca
     1261 gaagcaatcg gtttgacctg ggaagaccgc ccgaccgcgc tgcgaccgcc gctggagccg
     1321 gccggcacgc acgatctggt gaccgagatc cacccgcagg cgcaggcctt cgtcgccacc
     1381 gtggccgagg cgctgcggcg cggtggcgcc ggcgccgcct ggttcatcga ctacggcttt
     1441 ccggaagccg agtactacca cccgcagcgc agcgagggca ccctggtgtg ccaccgcgcg
     1501 caccgggtgg acgaccagcc gctggccgac gtgggcgaca aggacatcag cgcccacgtc
     1561 aacttcaccg gcgtggcgct ggcggcgcag gacgccggcc tggaggtgct gggctacacc
     1621 agccaggggc gctttctgct caactgtggc ctggccgagg cgctggccgc ggccgacctg
     1681 cccatgcgcg ccaacgccat gaaactgctg gacgagcacg agatgggcga gctgttcaag
     1741 gtcatcggac tggccacgcc cggccacccg ccggcgcgcg gctttgccca gggcgaccgc
     1801 acgcaccggc tgtgacacac atgatccgct ggctgatcgt cgtctttctg gccctggtgc
     1861 tgatcagcgg cctgacgccg ctgctgcgcc ggctgggcat cggccggctg ccgggggact
     1921 tgcactttcg gctgttcggg cgcgaatggt tcattccgct gaccagcacg ctgctgctga
     1981 gcgccctggc cagcctgatc gcgcgctggc tgtagggccg cgctgaagcg gctcagcgcc
     2041 ccggcgcgcg cgcgagcagc cgcaggtcgg gcccgacgcg ctcgatggcg tgccagtcga
     2101 acgccagcgc ctggtccagg ccggccagcg gccccaggtc ggcgatgccc acgccgtcgc
     2161 ccagcaggcg cggcgcctgg tacagcagca gctcgtccac caggccggcg cgcagcaggg
     2221 cgccgttgag ccggtggccg gcttccacgt gcagctcgtt cacctcctgc cgggccaggt
     2281 gcgccagcag ggccggcaga tcgacccggc cgtcgtcgcc cggcagcgcc agcacctggg
     2341 cgccgcgcgc ctgcagcggc tgctggcgtg cggcgtcggt gacggcggtg gcgatgcaca
     2401 cgcggcgccc ggccacctgc cacaggcgcg cctgcgcggg cgtgcgcagc tggctgtcga
     2461 gcaccaccag cgccggctgg cgcgggctgt cgggcaggcg cacgttgagc agcgggtcgt
     2521 cgtccagcac ggtgccgatg ccggtcagca cggcgcaggc gcgtgcgcgc caggcctgag
     2581 cgtcggcgcg cgcggcgggc gaggtgatcc attggctgcg gccgtcgggc agggcggtgc
     2641 gcccgtccag ggagctggcg accttcagcc gcacccaggg cgtgccgcgc tccatgcggg
     2701 tgaaaaagcc gatgttcagc tcgcgcgccg ccgcggccac ttcgggcggc cccagcgcca
     2761 cctcgacgcc ggcgtcgcgc agccgctgca ggccctggcc gccgacgcgc gggttcgggt
     2821 ccggcagcgc ggccaccacg cggcgcacgc cggccgcgac cagcgcgtcg cagcacggcc
     2881 cggtgcggcc gtggtgtgcg cagggctcca gcgtcacgta ggcggtggcg ccctgcgtgc
     2941 ggtgcccgcg cgcggcggca tcgcgcagcg ccatcacctc ggcatgcgcc tggccgaccg
     3001 gct
//
LOCUS       DAOQTZ010000190         2994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_38482_length_2994_cov_10.629126, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000190 DAOQTZ010000000
VERSION     DAOQTZ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38482_length_2994_cov_10.629126"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKN51_13610"
     CDS             complement(<1..488)
                     /locus_tag="PKN51_13610"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052418.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNT86181.1"
                     /translation="MNKPLTPAEAALRDAAREYHRNPKPGKIAVTPLKPLSNQLDLSL
                     AYSPGVAYPCLDIQADPSKAAEFTSRGNLVGVVTNGTAVLGLGDIGPLAGKPVMEGKG
                     CLFKKFAGIDVFDIELAERDPDKLVDIIASLEPTLGGINLEDIKAPECFYIERKLRER
                     MN"
     gene            857..1768
                     /locus_tag="PKN51_13615"
     CDS             857..1768
                     /locus_tag="PKN51_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNT86182.1"
                     /translation="MSTLTELQTPKRVRSIGHAPARHWVGDGFPVHGLFGYSGAGVAE
                     RSPFLLLDYAAPTEFAPTTQRRGVGSHPHRGFETVTIVYAGEVEHRDSAGGGGVIGAG
                     DVQWMTAGGGIIHEEFHSAAYAARGGTFEVVQLWVNLPARDKLTPARYQAITEAQIPV
                     VTLPDGAGCVRVIAGAYAGTRGPASTFSPMNVLDLRLAAGHTLALPQPEGWNTLVVVR
                     RGSLQVNGEAALRATEMATLSAAGRGLTLVAGDAADAELLVLAGQPIDEPVVGYGPFV
                     MNSREQIGQAIEDFNRGRFGRLGAGAG"
     gene            1938..2240
                     /locus_tag="PKN51_13620"
     CDS             1938..2240
                     /locus_tag="PKN51_13620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT86183.1"
                     /translation="MTTSPITLNESTRRFEQVVDGHTAYVEFERLPGQAIAYTHTVVP
                     EALGGRGIGSALVQHVMDYARQQGLQVRPDCSFIHAWIERHPAYQPLSLAHGATPR"
     gene            2339..2920
                     /locus_tag="PKN51_13625"
     CDS             2339..2920
                     /locus_tag="PKN51_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNT86184.1"
                     /translation="MPVDPVALHGRRAALALLSAGLLAGCASRPLPRASTSATASDGA
                     VTRRAPVAPVPPTQTPLALDPAAREAAVAQALLLINTPYTYGGNTPQGGFDCSGLMQY
                     VFQQVGGAATRLPRSTAQWAAATLPVDEARLARGDLVFFNTTGAPFSHMGLYVGGEQF
                     VHAPSSGGTVRKDSLASRYFATRYQGARRVFAA"
BASE COUNT          435 a         1066 c         1043 g          450 t
ORIGIN      
        1 atgttcatgc gctcgcgcag cttgcgctcg atgtagaagc actcgggcgc cttgatgtct
       61 tccaggttga tgccgcccag cgtcggctcc aggctggcaa tgatgtccac cagcttgtcg
      121 gggtcgcgct cggccagctc gatgtcgaac acgtcgatgc cggcgaactt cttgaacagg
      181 cagcccttgc cctccatcac cggcttgccg gccagcgggc cgatgtcgcc caggcccagc
      241 acggcggtgc cgttggtcac cacgcccacc aggttgccgc ggctggtgaa ttcggccgcc
      301 ttggacgggt cggcctgaat gtccaggcag gggtaggcca cgccgggcga gtaggccagc
      361 gacagatcga gctggttgga cagcggcttg aggggggtga ccgcgatctt gccgggtttg
      421 gggttgcggt ggtattcgcg cgcagcgtcg cgcagggcgg cttcggcggg ggtgagcggc
      481 ttgttcatgc ggtctccagt gcgttgtctt cgtggccggg aacgtgagcc tacatcagtt
      541 catccgcgtt gccgaggtgg gctggggccg cagaggcgcc aatcgtctgc cgccggcatg
      601 gcgccgtggt tgggtaaaaa aacgggctgc agcgcttgat ggtcaagcgc cagcagctat
      661 caaaatcaga gtatctgtgg tgcggaccgc ggtggccggg gcgggcgcgg gtggccacgg
      721 cgattgtttc gattctggaa cggtgtggcg gcaccgcgcc ccttggtggc cggccgctgc
      781 gcccctacca tcggagcatg ggcgtcgacc gtggggtcgg cgccgcgggc cgaccggccc
      841 tcaccggagc atcaccatgt ccaccctcac cgagcttcag acacccaagc gcgtgcggtc
      901 catcggtcac gcgcccgccc gccactgggt gggcgacggc tttccggtgc acggcctgtt
      961 cggctacagc ggtgcgggcg tggccgagcg cagccccttt ctgctgctgg actacgccgc
     1021 gcccaccgaa ttcgcaccca ccacgcagcg gcgcggcgtc ggcagccacc cgcaccgcgg
     1081 cttcgagacc gtgaccatcg tctacgcggg cgaggtcgag caccgcgact cggccggcgg
     1141 cggcggcgtg atcggcgccg gcgacgtgca gtggatgacc gccggcggcg gcatcatcca
     1201 cgaggagttc cactcggccg cctacgccgc gcgcggcggg acgttcgaag tggtgcagct
     1261 gtgggtcaac ctgccggcgc gcgacaagct gacgccggcg cgctaccagg ccatcaccga
     1321 ggcccagatt ccggtcgtga ccctgcccga cggcgccggt tgcgtgcgcg tgatcgccgg
     1381 cgcctatgcc ggcacccgcg ggccggccag caccttcagc ccgatgaacg tgctggacct
     1441 gcgcctggcc gccggtcaca cactggcgct gccccagccc gagggctgga acacgctggt
     1501 ggtggtgcgc cgcggcagcc tgcaggtcaa cggcgaggcg gccctgcgcg ccaccgagat
     1561 ggccaccctg tcggccgccg gccgcggcct gacgctggtg gccggcgacg ccgccgacgc
     1621 cgaactgctg gtgctggccg gccagccgat cgacgagccg gtggtcggct acggcccctt
     1681 cgtgatgaac agccgcgagc agatcggcca ggccatcgaa gacttcaacc gcgggcgctt
     1741 tggccgcctg ggggccgggg cgggctgaaa ccggcgattg cacaccggac atccgtacgc
     1801 agaggacgca gaacaaacct gaatgcgcct tggcgtcctc tgcgcaatct ctgggtcctc
     1861 tgcgtatgga tgttccggaa tgcccaccaa tgcagaacgc gacaccctgc cgccccaaca
     1921 ttcactgaac gcaaaccatg accacctccc ccatcaccct gaacgaatcc acccgccgct
     1981 tcgagcaggt ggtggacggc cacaccgcct atgtcgagtt cgagcgcctg cccggccagg
     2041 ccatcgccta cacccacacc gtcgtgcccg aggcgctggg cgggcgcggc atcggcagcg
     2101 cgctggtgca gcacgtgatg gactacgccc gccagcaggg cctgcaggtg cggcccgact
     2161 gcagcttcat ccacgcctgg atcgagcgcc accccgccta ccagccgctg tcgctggcgc
     2221 acggcgccac gccgcgctga ctggaccaga ccagaccggg ccggcgcgct ggacagggtt
     2281 cgcctgctat tcataaaata gctgctcgcg ctttctggac aagcgccgga gccctgaaat
     2341 gcctgtagac ccggttgccc tgcatggccg ccgtgcggcg ctggccctgc tgtcggccgg
     2401 cctgctggcc ggctgcgcca gccggccgct gccacgcgcc agcaccagcg ccaccgccag
     2461 cgacggcgcg gtgacccggc gcgcgcccgt cgccccggtg ccgcccacgc agacgccgct
     2521 ggcgctcgac ccggccgcgc gcgaagccgc cgtggcgcag gcgctgctgc tcatcaacac
     2581 cccctacacc tatggcggca acacgccgca gggcggcttc gattgcagcg gtctgatgca
     2641 gtatgtgttc cagcaggtcg gcggcgccgc cacacgcctg ccgcgcagca ccgcgcaatg
     2701 ggcggcggcc acgctgccgg tggacgaagc gcggctggcg cgcggcgacc tggtgttctt
     2761 caacaccacc ggcgcgccgt tttcgcacat ggggttgtac gtgggcggcg agcagttcgt
     2821 gcacgcgcct tcgagcggcg gcacggtgcg caaggacagc ctggccagcc gctacttcgc
     2881 cacccgctac cagggcgcgc ggcgggtgtt tgcggcatga acctagaaac ggcagttgcc
     2941 cgcttgccaa cctcgggcag accgcatcga tgcggggtct ggcggctgcg atgg
//
LOCUS       DAOQTZ010000191         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_44121_length_2733_cov_12.253921, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000191 DAOQTZ010000000
VERSION     DAOQTZ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44121_length_2733_cov_12.253921"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKN51_13630"
     CDS             <1..405
                     /locus_tag="PKN51_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HNT86185.1"
                     /translation="TNGPGLGLLPLVTSFEPQKTVRRTHARFADVAGPWSPLSGVAVQ
                     GYEIHSGQTAQHPAMAAKGDVAREVIPGLAWQSPAGNVLGCYLHGLFEDAAVLHALFG
                     AQAPTLDAVFDGLAAELERAFAPGALQALIAR"
     gene            429..1466
                     /gene="cobT"
                     /locus_tag="PKN51_13635"
     CDS             429..1466
                     /gene="cobT"
                     /locus_tag="PKN51_13635"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNT86186.1"
                     /translation="MSLNLPPVPDLHQPDLAAALRHKISRKTVPEGALGRMAALAERL
                     GLILGTAEPHLSAPQLLVCAADHGIAARGVSAYPSDVTWQMVGNFLAGGALVSVLARQ
                     HGIAQTVVDCGVRHDFAPAPGLVIRKIAPGTQDSSQGPAMSAAQCAQALANGIELVKA
                     LPGNALLLGEMGIGNTSAASLLVARLGGIDVAEVTGAGTGLDAAGIARKTAVLRQALA
                     ANAGATEPLDALAALGGFEIAILTGAVLQAASERRVIVVDGFIASSAVLVASRIAPAV
                     LQRCVFAHRSDERGHARLLGLLGAEPLLALGLRVGEGSGAVLAWPLLESSCRILNEMA
                     SFESAGVSDKA"
     gene            1514..>2733
                     /locus_tag="PKN51_13640"
     CDS             1514..>2733
                     /locus_tag="PKN51_13640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNT86187.1"
                     /translation="MKTLKRLLLALLLMLIVAAGIGWFSLDKEARGLLATFPTNRDIL
                     FWNEKQRDAAFRAMDRIALLAKWRTAAPSAQVRPLPPGPPLKLPLDVDAYLREQHGAA
                     LLILHKGQLRLERYGLGFDGSGRWTSFSVAKSFTSTLVGAALKDGHIKSLDDKVSAYI
                     PDMKGSAYDDVSIHQLLTMTSGIRWNEDYGDPNSDVARFNNHKPEPGVHALVSYLRQL
                     PRAAPPGTRWNYSTGETNLVGILISQAVGKPLTEYMQEKIWSRIGSEQQATWLLSKTG
                     QEISGCCIQAAPRDYARFGQFILEGAQVDGQPAAGPLPRPAAAPSGGSATGEAVERGG
                     AFILPEGYLQQATHKQADIGAPGRGYGYQWWTYDDGSFAARGIFGQGIFIDPARELVI
                     VTNGDWAKAGSANNP"
BASE COUNT          406 a          982 c          963 g          382 t
ORIGIN      
        1 accaacggcc ccggcctggg cctgctgccc ctggtgacca gcttcgagcc gcaaaaaacc
       61 gtgcgccgca cccacgcccg ctttgccgat gtggcgggcc cctggtcgcc gctgtcgggc
      121 gtggccgtgc agggctacga aatccacagc ggccagaccg cccagcaccc ggccatggcg
      181 gccaagggcg acgtggcgcg cgaagtcatc cccggcctgg cctggcagag cccggccggc
      241 aacgtgctgg gctgctacct gcacggcctg ttcgaagacg ccgccgtgct gcacgcgctg
      301 tttggcgccc aggcgccgac gctggacgcg gtgttcgacg gcctggccgc cgaactggag
      361 cgcgcctttg cgccgggtgc gctgcaggcg ctgatcgcgc gctgaatctt cgtttgttgc
      421 ttcaccccat gagcctgaac ctgccccccg tccccgatct gcatcaaccc gatctggccg
      481 ccgccctgcg tcacaagatc agtcgcaaga ccgtgcccga gggcgcgctc ggccgcatgg
      541 ccgcgctggc cgagcggctg ggcctgatcc tgggcacggc tgagccgcac ctcagcgcgc
      601 cgcaactgct ggtgtgcgcg gccgaccacg gcatcgccgc gcgcggcgtg tcggcctacc
      661 cgagcgacgt gacctggcag atggtgggca actttctggc cggcggcgcg ctggtcagcg
      721 tgctggcgcg ccagcacggt atcgcgcaga cggtggtgga ctgcggcgtg cgccacgact
      781 tcgcgcccgc gccggggctg gtgatccgca agatcgcgcc cggcacgcag gacagcagcc
      841 aagggccggc catgagcgcc gcgcagtgcg cgcaggccct ggccaacggc atcgagctgg
      901 tcaaggccct gcccggcaac gccctgctgc tgggcgagat gggcattggc aacaccagcg
      961 ccgcgtcgct gctggtggcg cgcctgggcg gcatcgacgt ggccgaggtt accggtgccg
     1021 gcaccgggct ggacgccgcc ggcatcgccc gcaagaccgc cgtgctgcgc caggcgctgg
     1081 ccgccaacgc gggcgccacc gagcccctgg atgcgctggc cgcgctgggc ggcttcgaga
     1141 tcgccatcct gaccggcgcc gtgctgcagg ccgccagcga gcggcgcgtg atcgtggtcg
     1201 atggcttcat cgccagcagc gcggtgctgg tggccagccg catcgcgccg gcggtgctgc
     1261 agcgctgcgt gttcgcccac cgctccgacg agcgcggcca cgcccgcctg ctgggtctgc
     1321 tgggcgccga gccactgctg gcgctgggcc tgcgcgtggg cgagggcagc ggcgccgtgc
     1381 tggcctggcc gctgctggag tcaagctgcc gcatcctcaa cgagatggcc agtttcgaat
     1441 cggccggcgt gtcggacaag gcctgaaggg cgggcgctac catgcgcgcc acgacgaacc
     1501 aaggagagct gtgttgaaaa ccctcaagcg actgctgctg gcgctgctgc tgatgctgat
     1561 cgtggccgcc ggcatcggct ggttcagcct ggacaaggag gcgcgcggcc tgctggccac
     1621 ctttccgacc aaccgcgaca tcctgttctg gaacgagaag cagcgcgacg ccgcctttcg
     1681 cgccatggac cgcatcgcgc tgctggccaa gtggcgcacc gccgcgccca gcgcgcaggt
     1741 gcggccgctg ccgcccggcc cgccgctcaa gctgccgctg gacgtggacg cctacctgcg
     1801 cgagcagcac ggcgcggcgc tgctgatcct gcacaagggc cagttgcggc tggagcgcta
     1861 cggcctgggc tttgacggca gcgggcgctg gaccagcttt tcggtcgcca agtccttcac
     1921 ctcgaccctg gtgggcgcgg cgctgaagga cgggcacatc aagagcctgg acgacaaggt
     1981 cagcgcctac atccccgaca tgaagggctc ggcctacgac gacgtcagca ttcatcaact
     2041 gctcaccatg acctcgggca tccgctggaa cgaggactac ggcgacccca actccgacgt
     2101 ggcgcgcttc aacaaccaca agcccgagcc gggcgtgcat gcgctggtca gctatctgcg
     2161 ccagctgccg cgcgcagcac cgccgggcac gcgctggaac tacagcaccg gcgagacgaa
     2221 tctggtcggc atcctgatca gccaggcggt gggcaagccg ctgaccgagt acatgcagga
     2281 aaagatctgg tcgcgcatcg gcagcgagca gcaggccacc tggcttttgt ccaagaccgg
     2341 gcaggagatc agcggctgct gcatccaggc cgcgccgcgc gactacgcgc gctttgggca
     2401 gttcattctg gaaggcgcgc aagtcgacgg ccagccggcc gccgggccgc tcccaaggcc
     2461 ggcggcggcc ccctcggggg gcagcgcaac cggcgaagcc gtggagcgtg ggggcgcttt
     2521 catcctgccc gagggctatc tgcagcaggc cacgcacaag caggccgaca tcggcgcgcc
     2581 gggccgcggc tacggctacc agtggtggac gtatgacgac ggcagctttg ccgcccgcgg
     2641 catctttggt cagggcatct tcatcgaccc ggcgcgcgag ctggtgatcg tcaccaacgg
     2701 cgactgggcc aaggccggct cggcgaacaa ccc
//
LOCUS       DAOQTZ010000192         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_45328_length_2685_cov_9.098859, whole genome shotgun sequence.
ACCESSION   DAOQTZ010000192 DAOQTZ010000000
VERSION     DAOQTZ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45328_length_2685_cov_9.098859"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..984)
                     /gene="prmC"
                     /locus_tag="PKN51_13645"
     CDS             complement(157..984)
                     /gene="prmC"
                     /locus_tag="PKN51_13645"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNT86188.1"
                     /translation="MNTLAAALRQWQLRGLPRLEAQMLLLHVLGQPTHARAWLLAHDS
                     QALPAAAAIRLHALAMRRLAGEPMAYLIGEKHFHGLRLQVDARVLDPRDDTETLVDWA
                     LALLPAEAPRRVLDLGTGSGAIALAIAHARPRARVTAVDASADALSVAQANAQRLQLA
                     LELRRGDWLAPVAGEVFDLIVSNPPYIAEGDAHLPALAHEPRSALVSGADGLDDLRRI
                     VAAAPAHLVPGGWLLLEHGWDQAGAVRDLLADAGFRQLQSRRDLAGIERCSGGQRAG"
     gene            complement(1060..1446)
                     /locus_tag="PKN51_13650"
     CDS             complement(1060..1446)
                     /locus_tag="PKN51_13650"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNT86189.1"
                     /translation="MSDHPALRHGAATLACALAALLAGCEGGTPWGATPQQIPLPPLP
                     RLEQLPAHIEAGTTAGAQRLAERVRAALAAEPRLAGATLDVQGFEGGVMVLTGQLPSA
                     AERALALQTARSVTGVGDVVDRMSAP"
     gene            complement(1482..2564)
                     /gene="prfA"
                     /locus_tag="PKN51_13655"
     CDS             complement(1482..2564)
                     /gene="prfA"
                     /locus_tag="PKN51_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNT86190.1"
                     /translation="MRAPLRHQLQRHAERLAELDFLLAQPDVQADTEQLMALSREHTE
                     VAAVAGRWARWQQRERDLAAAQELLGDADMADMAREEVAACQAELAALEAELQRLLLP
                     KDPDDARHAYLEIRAGTGGDESALFAGDLARMYTRYAQGRGWRVEVMSESPAELGGYK
                     ELVLHIVGDGAYGVLRFESGGHRVQRVPVTETQGRIHTSAATVAVLPEPDEAEAITLN
                     PAELRIDTFRASGAGGQHINKTDSAVRVVHLPTGMVAECQDGRSQHANKAKALRVLQA
                     RLQDKERSERAAREAATRKGLVGSGDRSDRIRTYNFPQGRLTDHRINLTLYKLDRVME
                     GDLQDVIDALRSAHEAEQLAELEAAA"
BASE COUNT          332 a          975 c          982 g          396 t
ORIGIN      
        1 gccgatcagc tgcgcctggc gctggccgac ctgtggcagg tcacgctgcc gctgcggctg
       61 gcgcgcatgg cgggttcagc gtccggcaag cgcatccagc tcagcagttt ttcgatcgac
      121 cccggcgata tgccataggt tgcacgcgaa gccgcatcag ccggcgcgct gcccgcccga
      181 gcagcgttcg atgcccgcca gatcgcgccg cgactgcagc tgccgaaagc cggcgtcggc
      241 cagcaggtcg cgcacggcgc ccgcctggtc ccagccatgc tccagcagca gccagccgcc
      301 cggcaccaga tgggccgggg cggcggccac gatgcggcgc aggtcgtcca gcccgtcggc
      361 gccgctgacc agcgcgctgc gcggctcgtg cgccagcgcc ggcaggtgcg catcgccttc
      421 ggcgatgtag ggcgggttgc tgacgatcag gtcgaacacc tcgcccgcca ccggcgccag
      481 ccagtcgccg cgccgcagct ccagcgccag ctgcagccgc tgggcgttgg cctgcgccac
      541 cgacagggcg tcggcgctgg cgtccaccgc cgtcacgcgc gctcgtggcc gggcgtgcgc
      601 aatggccagg gcgatggcgc cgctgccggt gcccaggtcg agcacgcggc gcggcgcctc
      661 ggccggcagc agcgccagcg cccagtcgac cagggtttcg gtgtcgtcgc gcgggtcgag
      721 cacgcgcgcg tccacctgca ggcgcaggcc gtggaaatgt ttttcaccga tcaggtaggc
      781 catcggctcg cccgccagcc ggcgcatggc cagcgcgtgc agacggatgg ccgccgcggc
      841 cggcagcgct tggctgtcgt gcgccagcag ccaggcgcgc gcgtgcgtgg gctggcccag
      901 cacgtgcagc agcagcatct gtgcctccag ccgcggcagg ccgcgcaatt gccactggcg
      961 cagggccgca gccagggtgt tcattgctat gctaaatata gctgctggcg ctttctgggc
     1021 aagcgctgga ggctgattcg accatcaaaa cgcggtgact cacggcgcgc tcatgcggtc
     1081 caccacgtcg cccacgccgg tcacgctgcg cgccgtctgc agcgccaggg cgcgttcggc
     1141 cgcgctgggc agttggccgg tgagcaccat caccccgcct tcaaagccct gcacgtccag
     1201 cgtcgcgccg gccaggcgcg gctcggccgc cagcgcggcg cgcacacgtt cggcaaggcg
     1261 ctgcgcgccg gcggtggtgc cggcctcgat gtgcgccggc aactgttcca gccgcggcag
     1321 cggcggcagc gggatctgct gcggcgtggc gccccagggc gtgccgccct cgcagccggc
     1381 cagcagggca gcgagcgcgc acgccagcgt ggcggcgccg tggcgaaggg cagggtggtc
     1441 gctcatggcg cggatgatag ccagccggcg ggggcggcgg ctcaggcggc cgcttccagc
     1501 tccgccagct gctcggcctc gtgggcgctg cgcagggcgt cgatcacgtc ctgcagatcg
     1561 ccttccatca cgcggtcgag cttgtacagc gtcaggttga tgcggtggtc ggtcagccgc
     1621 ccctgcggga agttgtaggt gcggatgcgg tcgctgcggt cgccgctgcc caccaggccc
     1681 ttgcgcgtgg cggcttccct ggcggcgcgt tcgctgcgct ccttgtcctg caggcgcgcc
     1741 tgcagcacgc gcagagcttt ggccttgttg gcgtgctggc tgcgcccgtc ctggcattcg
     1801 gccaccatgc cggtgggcag gtgcaccacg cgcacggccg aatcggtctt gttgatgtgc
     1861 tggccgccgg cgccgctggc gcggaaggtg tcgatgcgca gctcggccgg gttgagcgtg
     1921 atggcttcgg cctcatccgg ctcgggcagc accgccaccg tggcggcgct ggtgtggatg
     1981 cggccctgcg tttcggtcac cggcacgcgc tgcacgcggt ggccacccga ttcgaagcgc
     2041 agcacgccgt aggcgccatc gcccacgata tgcagcacca gctccttgta gccgcccagc
     2101 tcggccggcg actcgctcat cacctcgacg cgccagccgc ggccctgcgc gtagcgcgtg
     2161 tacatgcgcg ccagatcgcc cgcgaacagg gccgattcgt cgccgccggt gccggcgcgg
     2221 atctccaggt aggcgtggcg cgcgtcatcc gggtccttgg gcagcagcag gcgctgcagc
     2281 tcggcctcca gcgcggccag ctcggcctgg caggcggcga cttcttcgcg cgccatgtcg
     2341 gccatgtccg catcgcccag cagctcctgc gcggccgcca ggtcacgttc gcgctgctgc
     2401 cagcgcgccc agcgcccggc caccgccgcg acctcggtgt gctcgcgcga cagcgccatc
     2461 agctgctcgg tatcggcctg cacgtcgggc tgggcgagca ggaagtccag ttcggccagg
     2521 cgttcggcgt ggcgctgaag ttggtggcgg aggggggcgc gcatgaaaac acggaaaccg
     2581 gcgattttcg ccggtttgtt gaccgcacgg ttcggcagcg cttcagccgg agcggggatg
     2641 cgcggctact tgccccagca ttcggtcacg atggcgcccg aagtg
//
LOCUS       DAOQTZ010000193         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_48219_length_2582_cov_15.839731, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000193 DAOQTZ010000000
VERSION     DAOQTZ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48219_length_2582_cov_15.839731"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKN51_13660"
     CDS             complement(<1..660)
                     /locus_tag="PKN51_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HNT86191.1"
                     /translation="MNDRTQTLAYLDATRGGVASQEQRHRVLRNTYWLLALSLLPTVL
                     GAWLGVATGITAGLGGGLGLVVFLGGAFGFMFAIEKTKNSAAGVPVLLAFTFFMGLML
                     SRLIALVLGFKNGSELVMTAMGGTAGVFFVMASLASVIKRDLSGLGKWLFVGALVVFV
                     GAIVNVFVGSTVGMLVISTLVIAVFSLYMLYDLKRIVDGGETNYISATLALYLDLFNV
                     FQ"
     gene            806..1210
                     /locus_tag="PKN51_13665"
     CDS             806..1210
                     /locus_tag="PKN51_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNT86192.1"
                     /translation="MKTKPVLQAADVRAIVAAAEAEALKQQWAMTIAVADDGGHLLHL
                     HRMDGAAPVSAHIAPGKARSAALGRRESKGYEDMINGGRHAFLSVPLEGMLEGGLPIV
                     KDGQCIGAIGVSGAKPAEDTLVARAGIAALGL"
     gene            complement(1417..2238)
                     /locus_tag="PKN51_13670"
     CDS             complement(1417..2238)
                     /locus_tag="PKN51_13670"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNT86193.1"
                     /translation="MPQRTVFFVSDGTGITAETFGNAILAQFEFQPRHVRLPFIDTPD
                     KAHQAVRQINHTCELEGRRAIVFTTLVDREVMGIVREGCKGMLLDMFGTFVAPIEQEL
                     GITSHHRVGRFSDVSKNQAYQDRIEAINYSLAHDDGQSNRDLETADVILIGVSRSGKT
                     PTSLYLAMQHGVKAANYPLIPEDFERRQLPPALVPHKKKIFGLTIAPERLAEIRAERR
                     PNSRYASLENCRMEVGEAEAMMRRSGIRWLSSTTKSIEEIATTILQEVRPERLSY"
     gene            2407..>2582
                     /locus_tag="PKN51_13675"
     CDS             2407..>2582
                     /locus_tag="PKN51_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT86194.1"
                     /translation="MSARFAATALVVPFENLRMSDVEVVGGKNASLGEMISQLPQGVR
                     VPTGFATTAHAFRE"
BASE COUNT          427 a          820 c          898 g          437 t
ORIGIN      
        1 ctggaacacg ttgaacaggt ccaggtacag cgccagcgtg gcgctgatgt agttggtttc
       61 gccgccgtcg acgatgcgct tgaggtcgta cagcatgtac aggctgaaca cggcgatcac
      121 cagggtgctg atgaccagca tgccgaccgt ggagccgacg aacacgttga cgatggcgcc
      181 gacgaagacc accagggctc ccacgaacag ccatttgccc aggcccgaca ggtcgcgttt
      241 gatgacgctg gccaggctgg ccatgacgaa gaacacgccg gctgtgccgc ccatggcggt
      301 catcaccagt tccgagccgt tcttgaagcc gagcaccagg gcgatcagac gcgacagcat
      361 caggcccatg aagaaggtga aggccagcag cacgggcacg ccggcggccg agttcttggt
      421 tttttcgatc gcgaacatga agccgaaggc gccgcccaga aacaccacca gacccaggcc
      481 tccgcccagt ccggccgtga tgccggtggc cacgcccaac caggcgccca gcacggtggg
      541 cagcaggctc agcgccagca gccagtaggt gttgcgcagc acgcggtggc gctgctcctg
      601 gcttgccacg ccgccccggg tggcgtccag gtaggccagg gtctgggtgc gatcgttcat
      661 gtctcggttg ctccttggat acccgctgcg gccgccactg cggcgacctt cgggggcatt
      721 ttacgtagag tcggtgccgg cgcatcggtt ccctggccgg cggttatgct tgcgggcatc
      781 ccgttcaagc ctggagcccg cacccatgaa gaccaaacct gtgctgcaag ccgccgacgt
      841 gcgcgccatc gttgccgccg ccgaagccga ggcgttgaag cagcaatggg ccatgaccat
      901 cgccgtggcc gacgacggcg gccatctgct gcacctgcac cgcatggacg gtgcggcgcc
      961 cgtgagcgcc cacatcgcgc cgggcaaggc gcgcagcgcc gcgctggggc ggcgtgagag
     1021 caagggctac gaggacatga tcaacggtgg ccgccacgcc tttctgtcgg tgccgctgga
     1081 gggcatgctg gaaggcgggc tgccgatcgt caaggacggc cagtgcatcg gtgccatcgg
     1141 agtgagcggt gccaaacccg cggaggacac gctggtggcc cgggccggga tcgcggcgct
     1201 cggtctctga gctcaggacg gccgccgggc gcgccccccg gtgtcgcgct ggccgagcca
     1261 ggccagggcg ctgccgtcgc gaagctggtg cccgcaggat ccgggtccag aggctcgaat
     1321 cggtgtcaaa acggccacca gcgcttgcca gacaagcgcc aaaagctatg aaaaggatag
     1381 tcttcgtggc gctccagacg ccgcgtcgga gggcgttcag tagctgagcc gctcgggccg
     1441 cacttcctgc aggatggtgg tggcgatttc ctcgatcgac ttggtggtcg acgacagcca
     1501 gcgtatgcct gagcggcgca tcatggcctc ggcctcgccc acttccatgc ggcagttttc
     1561 cagactggcg tagcgcgagt tggggcggcg ctcggcgcgg atttcggcca gccgctcggg
     1621 cgcgatggtc aggccgaaga tcttcttctt gtgcggcacc agggccggcg gcagctggcg
     1681 gcgctcgaag tcctcgggga tcagcgggta gttggcggcc ttgaccccgt gctgcatcgc
     1741 caggtagagg ctggtggggg tcttgccgct gcggctgacg ccgatcagga tgacgtcggc
     1801 ggtttcgagg tcgcggttgc tctggccgtc gtcatgggcc aggctgtagt tgatggcttc
     1861 gatgcgatcc tggtaggcct ggttcttgct gacgtccgaa aagcgtccca cccggtggtg
     1921 gctggtgatg cccagttcct gctcgatcgg cgccacgaag gtgccgaaca tgtccagcag
     1981 catgcccttg cagccctcgc gcacgatacc catcacctcg cggtcgacca gggtggtgaa
     2041 gacgatcgcc cgccgcccct ccagctcgca ggtgtggttg atctggcgca ccgcctggtg
     2101 cgccttgtcc ggcgtgtcga tgaagggcag gcgcacgtgg cggggctgga attcgaactg
     2161 cgccaggatg gcgttgccga aggtttcggc cgtgatgccg gtgccgtcgg agacgaaaaa
     2221 cacggtgcgt tggggcatgg gtgagggcgg cgtggcggtc aatacaatcg tgcccattat
     2281 gcgaacccgc gccgctggcc gttgcgaccc ccgaaaatgc agaacatggg ttgcgaatga
     2341 tgcgaatgcg ccgccaaggc ccgagggcgt tcgcagccac cggttttcaa actctggagc
     2401 tttcccatgt ctgcacgttt tgcggcgacc gccctggtcg taccgtttga gaacctgaga
     2461 atgagcgacg tcgaggtcgt tggcggcaag aacgccagcc tcggcgagat gatctcgcag
     2521 ctgccccagg gcgtgcgcgt gcccaccggc tttgccacca cggcgcacgc cttccgcgaa
     2581 tt
//
LOCUS       DAOQTZ010000194         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_50139_length_2520_cov_13.458418, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000194 DAOQTZ010000000
VERSION     DAOQTZ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50139_length_2520_cov_13.458418"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKN51_13680"
     CDS             complement(<1..711)
                     /locus_tag="PKN51_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT86195.1"
                     /translation="MASAPQVAFFSPLGAAFGVDLGACLQRVVDSGWYVLGPQVREFE
                     TAFARHVGVRHAVGVANGTDALTLALRALGIQPGQRVLTVANAGCYSSTAMAQVGADP
                     GWVDVDPARLTMAPEALAAALAEGRVAAVIVTHLYGQMADMPRLTALCQAAGVPLIED
                     CAQAHGAALQGRRAGAWGTLACFSFYPTKNLGALGDGGAVLTDDAALAERLRALRQYG
                     WTRKYEVGLPGGGNSRLDE"
     gene            complement(713..1612)
                     /gene="rfbA"
                     /locus_tag="PKN51_13685"
     CDS             complement(713..1612)
                     /gene="rfbA"
                     /locus_tag="PKN51_13685"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNT86196.1"
                     /translation="MNARKGIILAGGSGTRLHPATLAMSKQLLPVYDKPMIYYPLSTL
                     MLAGMRDILVISTPQDTPRFAQLLGDGSQWGITIQYAVQPSPDGLAQAFLIGESFIGG
                     APSALVLGDNLFYGHDFELLLSNAHARSEGATVFAYHVHDPERYGVVAFDAAGRATSI
                     EEKPAQPKSNYAVTGLYFYDPQVVDIAKTIRPSARGELEITTVNETYLKQGQLNVQIM
                     QRGYAWLDTGTHDSLLDAGQFIATLERRQGLKIACPEEVAWRKRFIDDAQLERLAAPL
                     AKSGYGQYLRRLLQEPAQPAERR"
     gene            complement(1609..2517)
                     /gene="rfbD"
                     /locus_tag="PKN51_13690"
     CDS             complement(1609..2517)
                     /gene="rfbD"
                     /locus_tag="PKN51_13690"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNT86197.1"
                     /translation="MKILLLGKNGQLGWELQRSLAPLGELVALDRRGAPGLCGDLGEP
                     DGLAATVRQLCPDVIVNAAAYTAVDRAESEPELARRINAHAPAVLAREAAACGALLVH
                     YSTDYVFHGQGQRPWAEDDPTGPLNVYGQTKLEGEQAILRSAGRHLILRTSWVYAARG
                     GNFARTMLRLARQQPRLTVIDDQWGAPTGAELIADVSAHAIVQLRARPDKAGVYHLAA
                     AGETNWFSYAKHVLARAQSAQPAIEIVANEILPIPSHAYRVAARRPHNSRLDTRRLRS
                     TFDLHLPPWQAGVDRMLAEILGPSHA"
BASE COUNT          347 a          827 c          950 g          396 t
ORIGIN      
        1 ctcgtccaga cggctgttgc caccaccggg cagaccgact tcgtacttgc gtgtccagcc
       61 gtactggcgc agggcgcgca ggcgttcggc cagcgcggcg tcgtcggtca acacggcgcc
      121 gccgtcgccc agggcgccca ggttcttggt ggggtagaag ctgaaacagg cgagcgtgcc
      181 ccaggcgccg gcgcggcgcc cctgcagcgc agcaccatgc gcctgggcgc agtcttcgat
      241 cagcggtacg ccggcagcct ggcacagcgc cgtcaggcgc ggcatgtcgg ccatctggcc
      301 gtacaggtgg gtgacgatga cagccgccac gcggccctcc gccagcgcag cggccagcgc
      361 ctcgggcgcc atcgtcaggc gcgcgggatc gacatcgacc cagccgggat cggcccccac
      421 ctgggccatg gcggtgctgc tgtaacaccc tgcattggcc acggtgagca cgcgctggcc
      481 gggttgtatg cccagggcgc gcagggccag cgtcagggcg tcggtgccgt tggccacgcc
      541 caccgcgtgc cgcacgccga catgccgggc aaacgccgtc tcgaattcgc gcacctgcgg
      601 ccccagcacg taccagccgc tgtcgaccac gcgctgcaga caggcgccca gatcgacgcc
      661 gaaggcggcc ccgagcgggg agaagaacgc cacctgcggc gcgctggcca tgtcagcgcc
      721 gctccgccgg ctgggccggc tcctgcagca agcgccgcag gtactggccg tagccgcttt
      781 tggccagcgg cgccgccagg cgttcgagct gggcgtcgtc gatgaagcgc ttgcgccagg
      841 cgacttcttc cgggcaggcg atcttcaggc cctggcgccg ctccagggtg gcgatgaact
      901 ggccggcatc gagcaggctg tcgtgggtgc cggtgtccag ccaggcgtag ccgcgctgca
      961 tgatctgcac gttgagctgg ccctgcttga ggtaggtttc gttgacggtg gtgatttcca
     1021 gctcgccgcg cgcgctgggt cggatggtct tggcgatgtc gaccacctgc gggtcgtaga
     1081 agtacaggcc ggtgacggcg tagttgcttt tgggctgcgc gggtttttct tcgatgctgg
     1141 tggcgcggcc ggcggcgtcg aaggcgacca cgccgtagcg ctcggggtcg tgcacgtggt
     1201 aggcgaacac ggtggcgcct tcgctgcgcg catgggcgtt ggagagcagc agctcgaagt
     1261 cgtggccgta gaacaggttg tcgcccagca ccagggcgct gggggcgccg ccgatgaagg
     1321 attcaccgat cagaaaggcc tgggccagcc cgtcggggct gggctgcacg gcgtactgga
     1381 tagtgatgcc ccactggctg ccatcgccca gcagttgcgc aaagcgtggc gtgtcctgcg
     1441 gtgtgctgat gaccaggatg tcgcgcatgc cggccagcat cagcgtggac agcgggtagt
     1501 agatcatcgg cttgtcgtac accggcagca gctgcttgct catggccagg gtggccgggt
     1561 gcaggcgggt gccggagccg ccggccagga tgatgccctt gcgtgcgttc atgcgtggga
     1621 aggtccaagg atttcggcca gcatgcggtc cacgccggcc tgccagggtg gcaggtgcag
     1681 atcgaaggtc gagcgcagcc ggcgcgtgtc gaggcgcgaa ttgtgcggcc gccgggccgc
     1741 cacgcgatag gcgtggctgg ggatgggcag gatctcgtta gctactattt caatagctgg
     1801 ttgcgcagac tgggcgcgcg ccagaacgtg ttttgcatag gaaaaccagt tggtttcgcc
     1861 ggccgccgcc aggtggtaga cgccggcctt gtcgggccgc gcgcgcagct gcacgatggc
     1921 gtgcgcgctg acgtcggcaa tcagctcggc accggtgggc gcgccccact ggtcgtcgat
     1981 cacggtcagg cgcggctgct ggcgcgccag gcgcagcatg gtgcgggcaa agttgccgcc
     2041 gcgcgcggcg tagacccagc tggtgcgcag gatcaggtgg cgaccggcgc tgcgcaggat
     2101 ggcctgctcg ccctccagct tggtctggcc gtagacgttc agcgggccgg tggggtcgtc
     2161 ctcggcccag ggccgctggc cctggccgtg aaacacatag tcggtgctgt agtgcaccag
     2221 cagcgcgccg caggcggcgg cctcgcgcgc cagcacggcc ggcgcgtggg cgttgatgcg
     2281 gcgcgccagt tcgggctcgc tttcggcgcg gtcgacggcg gtgtaggcgg cggcgttgac
     2341 gatgacgtcg gggcacagct ggcgcaccgt ggcggccagg ccgtcgggtt cgcccaggtc
     2401 gccgcacagg ccgggcgcgc cgcggcgatc cagcgccacc agctcgccca gcggcgccag
     2461 gctgcgctgc agctcccagc cgagctggcc gttcttgccc agcagcagga tcttcatggg
//
LOCUS       DAOQTZ010000195         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_50190_length_2518_cov_13.984978, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000195 DAOQTZ010000000
VERSION     DAOQTZ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50190_length_2518_cov_13.984978"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2382
                     /gene="alaS"
                     /locus_tag="PKN51_13695"
     CDS             <1..2382
                     /gene="alaS"
                     /locus_tag="PKN51_13695"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNT86198.1"
                     /translation="GYTARHHTFFEMLGNWSFGDYFKKESIQWGWELLTQVYKLPPER
                     LLATVYHEDDEAYDIWTKVIGLPPERVIRIGDNKGGKYKSDNFWMMADTGPCGPCSEI
                     FYDHGPHIPGGPPGSPDEDGDRFIEIWNHVFMQFDMKEDGSVVRLPAPCVDTGMGLER
                     LAAILQHVHSNYEIDLFARLIAEAARVTGCTDLANPSLKVIADHIRATAFLVSDGVIP
                     SNEGRGYVQRRIIRRAIRHGYKLGCKAPFFHKLVPLLVELMGAAYPRLQAEAQRVQQV
                     LKTEEERFFETLANGMDILDDALIAGRKELPGHIAFKLHDTYGFPLDLTQDVCRERGV
                     LVDVAGFDEAMAAQKAAGRAAGKFRMDKAVDYDGPGNAFIGYEHLRGEARVLALYHEG
                     SATPALREGQHGIVVLDRTPFYAESGGQVGDQGTLWIEHHGEPAFVVQDTQKIKADVF
                     GHHGTARKGGLKLGDAVIAHVDPDTRAATMRNHSVTHLMHKALREVLGAHVQQKGSLV
                     DADKTRFDFAHHGPVTPEQMADIERRVNAEILANAATQARLMDIESAQQTGAMMLFGE
                     KYGDTVRVLDIGSSRELCGGTHVARTGDIGLFKIVSEGGVAAGVRRIEAITGEHALGY
                     LQHLEASLHGVAATLRAPAGEVQARLGQVLDQVKALEKEIAQLKGKLASSQGDELVNQ
                     AVEVKGLKVLAARLDGADAKTLRDTLDKLKDKLGSAAIVLATVDGDKVQLAAGVTRDA
                     VGRVKAGELVSHVARQVGGKGGGKPDMAMAGGTDAKALPVALASVQAWVAERV"
BASE COUNT          456 a          880 c          859 g          323 t
ORIGIN      
        1 ggctacaccg cgcgccacca caccttcttc gagatgctgg gcaactggtc gttcggcgac
       61 tacttcaaga aggagtccat ccagtgggga tgggagcttc tgacgcaggt ctacaaactg
      121 ccgcccgagc gcctgctggc caccgtctac cacgaggacg acgaggccta cgacatctgg
      181 acgaaagtga tcggcctgcc gcccgagcgc gtgatccgca tcggcgacaa caagggcggc
      241 aagtacaaga gcgacaactt ctggatgatg gccgacaccg gcccctgcgg cccgtgcagc
      301 gaaatcttct acgaccacgg cccgcacatc cccggtggcc cgcccggcag cccggacgag
      361 gacggcgacc gcttcatcga gatctggaac cacgtgttca tgcagttcga catgaaggaa
      421 gacggcagcg tggtcaggct gcccgcgccc tgcgtggaca ccggcatggg cctggagcgc
      481 ctggccgcca tcctgcagca cgtgcacagc aattacgaga tcgacctgtt cgcacgcctg
      541 atcgccgagg ccgcgcgcgt gacgggttgc accgacctgg ccaacccctc gctgaaggtg
      601 attgccgacc acatccgcgc caccgccttc ctggtcagcg acggcgtgat ccccagcaac
      661 gaagggcgcg gctacgtgca gcggcgcatc atccgccgcg ccatccgtca cggctacaag
      721 ctcggctgca aggcgccgtt cttccacaag ctggtgccgc tgctggtcga gctgatgggc
      781 gcggcctacc cgcgcctgca ggccgaggcc cagcgcgtgc agcaggtgct caagaccgag
      841 gaggagcgct tcttcgagac cctggccaac ggcatggaca tcctggacga tgcgctgatc
      901 gccggccgca aggagctgcc cgggcacatc gccttcaagc tgcacgacac ctacggcttc
      961 ccgctcgacc tgacgcagga cgtgtgccgc gagcgcggcg tgctggtcga cgtggccggt
     1021 ttcgacgagg ccatggcggc gcaaaaggcc gccggccgcg cggccggcaa gttccgcatg
     1081 gacaaggcgg tcgactacga cggccccggc aacgccttca tcggctacga acacctgcgc
     1141 ggcgaagcca gggtgctggc gctctaccac gaaggcagcg ccaccccggc cctgcgcgag
     1201 gggcagcacg gcatcgtggt gctcgaccgc acgccgttct atgccgaaag cggcggccag
     1261 gtgggcgacc agggcacact gtggatcgag caccatggcg agccggcgtt cgtggtgcag
     1321 gacacgcaga agatcaaggc cgacgtgttc ggccaccacg gcacggcgcg caagggcggg
     1381 ctgaagctgg gcgatgcggt gatcgcccat gtcgaccccg acacgcgcgc cgccaccatg
     1441 cgcaaccata gcgtcaccca cctgatgcac aaggcgctgc gcgaggtgct gggcgcgcac
     1501 gtgcagcaga agggcagcct ggtcgatgcc gacaagacgc gcttcgactt cgcccaccat
     1561 gggccggtga cgcccgagca gatggccgac atcgagcgcc gcgtgaacgc cgaaatcctg
     1621 gccaacgccg ccacccaggc gcgcctgatg gacatcgaaa gcgcccagca gaccggcgcc
     1681 atgatgctgt tcggcgaaaa atacggcgac accgtgcgcg tgctcgacat cggctcaagc
     1741 cgcgagctgt gcggcggcac gcacgtggcg cgcaccggcg acatcggcct gttcaagatc
     1801 gtcagcgaag gcggcgtggc cgccggcgtg cgccgcatcg aggccatcac gggcgagcac
     1861 gcgctgggct acctgcagca cctggaagcc agcctgcacg gcgtcgccgc caccttgcgc
     1921 gcacccgctg gcgaagtgca ggcgcgcctg ggccaggtgc tcgaccaggt caaggcgctg
     1981 gaaaaggaaa tcgcccagct caagggcaag ctggcctcca gccagggcga tgaactggtg
     2041 aaccaggcgg tcgaggtcaa gggcctgaag gtgctggccg cccggctcga cggggccgac
     2101 gccaaaaccc tgcgcgacac cctggacaag ctcaaggaca agctgggcag cgccgccatc
     2161 gtgctggcca ccgtcgatgg cgacaaggtg cagctggccg ccggcgtcac ccgggacgcc
     2221 gtgggccgcg tgaaggcggg cgagctggtc agccatgtgg cccggcaggt gggcggcaag
     2281 ggcggcggca agcccgacat ggccatggcc ggcggcaccg acgccaaggc gctgcccgtg
     2341 gcgctcgctt cagtccaggc ctgggtggcc gagcgcgtct gaggcgccag ccggccgccg
     2401 ccttaccggt tgaccttgcc gggcagcgac aggtcgcccg acgccacctt gaacacattc
     2461 accacgcaca gcagcgggct gtgcacgctg gcgttgacgc gcagcgccgc ggtcacgc
//
LOCUS       DAOQTZ010000196         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_53910_length_2407_cov_13.872024, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000196 DAOQTZ010000000
VERSION     DAOQTZ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53910_length_2407_cov_13.872024"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..88)
                     /locus_tag="PKN51_13700"
     tRNA            complement(3..88)
                     /locus_tag="PKN51_13700"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Tyr,seq:gta)
     gene            complement(113..880)
                     /locus_tag="PKN51_13705"
     CDS             complement(113..880)
                     /locus_tag="PKN51_13705"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNT86199.1"
                     /translation="MLNPAGQGAALGQWPCDLDEVAAGWRPLLQAFLASAEGRRLSEG
                     LRARLAAGATIYPPQPLRALQLTPPEAVRAVILGQDPYHGPGQACGLAFAVAPGVRPP
                     PSLRNIFKELAREFGRPPQTEQHALVHWARQGVLLLNTSLTVEAGQAASHARIGWQML
                     TSQIFHRLVHGPRPLAFLLWGAHAQALRPQGAEAGRHLWLVANHPSPLSALRPPVPFI
                     GCGHFGRVNSFLRQQGEPEIDWLGTAAPAGPLSGPAV"
     gene            complement(938..1741)
                     /gene="trpC"
                     /locus_tag="PKN51_13710"
     CDS             complement(938..1741)
                     /gene="trpC"
                     /locus_tag="PKN51_13710"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNT86200.1"
                     /translation="MSDILDRIVAVKREEVAAGLKKKSLAAMRADAESRVLTRDFVGA
                     LRAKVAAGQAAVIAEIKRASPSKGVIRPGEFIPADLAQSYAQGDGQISAACLSVLTDR
                     QFFQGGIDYLKQARASCGLPVLRKDFMIDPWQVYEGRAIGADAILLIAACLSDGQMAE
                     LEAVALGLDMAVLVEVHDGAELQRALRLKTPLVGINNRNLRSFDTTLETTLGLLADVP
                     ADRLLVTESGIASRADVQRLRAAGVQAFLVGETFMRADEPGLALAELFR"
     gene            complement(1734..1877)
                     /locus_tag="PKN51_13715"
     CDS             complement(1734..1877)
                     /locus_tag="PKN51_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT86201.1"
                     /translation="MWQDEGTWIALGISALFLVAALVMHRVFVKILKNPAPDERKELS
                     NDE"
     gene            complement(1889..>2407)
                     /gene="trpD"
                     /locus_tag="PKN51_13720"
     CDS             complement(1889..>2407)
                     /gene="trpD"
                     /locus_tag="PKN51_13720"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803012.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNT86202.1"
                     /translation="MGVKTFFNILGPLTNPAGAPNILMGVFHPDLVGIQVRALQRLGA
                     EHAVVVYGRDGMDEVSLGAATLVGELKDGEIREYEIHPEDFGLRMQSNRSLRVETPEQ
                     SVAMLRGVLDNQEGAARDIVALNAGVVLYAAGVAPSMMAGIERASQAIESGAARAKLA
                     QVVALTQSLAAA"
BASE COUNT          360 a          858 c          826 g          363 t
ORIGIN      
        1 gctggtggag agggctggat tcgaaccagc gtaggtataa accggcagat ttacagtctg
       61 ctgccattaa ccactcggcc acctctccgg cagagaactt gcgattatgc catcaaaccg
      121 ccgggccaga caaggggccg gcgggcgcgg ccgtccccag ccagtcgatc tccggctcgc
      181 cctgctggcg caggaagctg ttgacgcgcc cgaaatgccc gcagccgatg aagggcacgg
      241 gcgggcgcag cgccgacagc ggtgacggat ggttggcaac cagccagagg tggcgcccgg
      301 cctcggctcc ctggggccgc agtgcctggg catgcgcacc ccacagcagg aacgccaacg
      361 gtcgtgggcc gtgcaccagc cggtgaaaga tctgcgaagt cagcatctgc cagccgatgc
      421 gggcgtggct ggcggcctgg cccgcttcga cggtgaggct ggtgttgagc agcagcacgc
      481 cctggcgcgc ccagtgcacc agggcgtgct gctcggtctg gggcggccga ccgaattcgc
      541 gcgccagctc cttgaagatg ttgcgcaggc tgggcggcgg gcgcaccccg ggggcgacgg
      601 caaacgccag accgcaggcc tgcccgggcc cgtggtacgg gtcctgaccg aggatgacgg
      661 cgcgcaccgc ctcgggcggg gtcagttgca gcgcgcgcag cggctgtggc gggtagatgg
      721 tggcaccggc cgccagccgc gcccgcagcc cttcgctcag ccgccggcct tcggcgctgg
      781 ccagaaacgc ctgcagcagc gggcgccagc cggccgccac ctcgtccagg tcgcagggcc
      841 actggcccag cgcggcgccc tgcccggctg gattcagcat cccatgggag tccaccgctt
      901 acccagattg cgccgtcagc tatcttaatg atagcgttca gcgaaacagt tcggccagcg
      961 ccagccccgg ctcgtcggcg cgcatgaagg tttcgccgac caggaaggcc tgcacgccgg
     1021 cagcgcgcag acgctgcacg tcggcgcggc tggcgatgcc gctttcggtg accagcaggc
     1081 ggtcggccgg cacgtcggcc agcagcccca gcgtggtctc cagcgtggtg tcgaagctgc
     1141 gcaggttgcg gttgttgatg ccgaccagcg gggttttcag ccgcagcgcg cgttgcagtt
     1201 cggcgccgtc gtgcacctcg accagcaccg ccatgtccag gcccagcgcc accgcttcca
     1261 gctcggccat ctggccgtcg gacaggcagg cggcgatcag caggatggcg tcggcgccga
     1321 tggcgcgccc ctcgtacacc tgccagggat cgatcatgaa gtccttgcgc agcaccggca
     1381 ggccgcagct ggcgcgcgcc tgcttcaggt agtcgatgcc gccctggaag aactggcgat
     1441 cggtcagcac cgacagacag gcggcgctga tctggccgtc gccctgggcg tagctctgcg
     1501 ccaggtcggc ggggatgaac tcgccgggcc ggatcacgcc cttgctgggg ctggcgcgct
     1561 tgatctcggc gatcacggcg gcctggccgg ccgccacctt ggcgcgcagc gcgcccacga
     1621 agtcgcgcgt cagcacacgg ctttcggcgt cggcgcgcat cgccgccagc gacttcttct
     1681 tcaggccggc ggccacttcc tcgcgcttga cggccacgat ccggtccaga atgtcactca
     1741 tcatttgata gctccttgcg ctcatcgggc gccgggtttt tcagaatctt gacgaacacg
     1801 cggtgcatca ccagcgccgc caccagaaac agggccgaga tgcccagggc gatccaggtg
     1861 ccttcgtcct gccacatggc gccaccgatc aggccgccgc cagcgactgc gtcagcgcca
     1921 ccacctgcgc cagcttggcc ctggcagcgc cgctttcgat ggcctgggac gcgcgctcga
     1981 tgccggccat catgctgggc gccacgcccg ccgcgtacag caccacgccg gcgttgagcg
     2041 ccacgatgtc gcgcgccgcg ccctcctggt tgtccagcac gccgcgcagc atggcgaccg
     2101 attgctccgg cgtctcgacc cgcagactgc ggttgctctg catgcgcagg ccgaagtcct
     2161 cggggtggat ctcgtactcg cggatttcgc cgtccttgag ctcgcccacc agggtggcgg
     2221 cgcccaggct gacttcgtcc atgccgtcgc ggccgtacac caccaccgcg tgctcggcgc
     2281 ccaggcgctg cagcgcgcgc acctggatgc ccaccagatc ggggtgaaac acccccatca
     2341 ggatgttggg cgcgccggcc gggttggtga gcggccccag gatgttgaag aaggtcttga
     2401 cgcccat
//
LOCUS       DAOQTZ010000197         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_53985_length_2405_cov_12.839574, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000197 DAOQTZ010000000
VERSION     DAOQTZ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53985_length_2405_cov_12.839574"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKN51_13725"
     CDS             <1..600
                     /locus_tag="PKN51_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNT86203.1"
                     /translation="WLVGLVVVLARRGDARGQRANLWGLALGTAVLLAGVGLQARVEH
                     LARASLAAQGVHPERVLVTPAPLSMLLWRVVAMEGDGYYEGFHGLLDGPGPMRFDRFD
                     RGARLEAAVAAIDGAQRIRRFSHGFYKLHESAGGQLVITDLRMGQEPHYSFAFAVAQR
                     GSPFAPLPVAQTVGGAVPVGPALAWLGRRMRGEQVAPPR"
     gene            611..1642
                     /locus_tag="PKN51_13730"
     CDS             611..1642
                     /locus_tag="PKN51_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT86204.1"
                     /translation="MTLQATVSMSWVDAVLAEAERQGVGRGRLLAQAGIAPEELQQER
                     WPIDHITRLWHAAVACTQDAGFGLAAGSRAGPDNFNVVGELLRASATLREAIAAVQKY
                     QRLISDGGRFQMVAGARASWLIYHPRQGTLAFSPHQVEAVLATVLAFVRNALGQRPGA
                     GVRPLQVQFSQPRVGPLAGYREVFACPLAFEQAFNGLLLDNAVLDAPLPHADPHRARA
                     HQREAAAHLAGLSQGGALAQELRAWMAAALASRVPTRAEAAAALGVSERTLARRLRAQ
                     GISFAELLDGVRRDAALQAVGQTTRPLADIGQALGFAEPAVFWRAFKRWSGSTPAAWR
                     AAQRADRQP"
     gene            complement(2018..>2405)
                     /locus_tag="PKN51_13735"
     CDS             complement(2018..>2405)
                     /locus_tag="PKN51_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNT86205.1"
                     /translation="NRRFEPMAIPAVCFTDPEVVVTGKTPGEARAAGMDCIDAAFPFA
                     ANGRAMTLEAASGFVRVVARRDNHLILGWQAVGKGVAELAAAFSQSIEMGARLEDVGH
                     TIHAHPTLGEAVQEAALRALGQALHI"
BASE COUNT          299 a          817 c          928 g          361 t
ORIGIN      
        1 tggctcgtcg gcctggtggt ggtgctggcg cgccggggcg atgcgcgtgg ccagcgcgcc
       61 aatctgtggg ggctggcgct gggcacggcg gtgctgctgg ccggtgtggg gctgcaggcg
      121 cgggtggagc atcttgcgcg cgccagcctg gccgcgcagg gcgttcatcc tgagcgcgtg
      181 ctggtcacgc ccgcgccgct gtccatgctg ctgtggcgcg tggtggctat ggagggcgat
      241 ggctactacg agggctttca tggcctgctc gatgggcccg ggcccatgcg ctttgaccgc
      301 tttgaccgcg gcgcgcgcct ggaagcggcg gtggccgcca tcgacggcgc gcagcgcatc
      361 cggcgcttca gccacggctt ttacaagctg cacgagagcg ccggcggcca gctggtgatc
      421 accgacctgc gcatggggca ggagccgcat tacagctttg cctttgcggt ggcgcagcgc
      481 ggatcgccct ttgcgccgct gccggtggcg cagacggtgg gtggcgccgt gccggtcggg
      541 ccggcgctgg cctggctggg tcggcgcatg cggggcgagc aggtggcgcc gccgcgttga
      601 gggccgggcc atgaccttgc aggccacggt ctccatgagc tgggtcgatg cggtgctggc
      661 cgaggccgag cgccagggcg tggggcgtgg gcgcttgctg gcgcaggccg gtatcgcacc
      721 cgaagaactg cagcaagagc gctggccgat cgaccacatc acgcgcctgt ggcacgcggc
      781 ggtggcctgc acacaggacg cgggctttgg tctggccgcc ggcagccgcg ccgggccgga
      841 caacttcaac gtggtgggcg agctgctgcg cgccagcgcc acgctgcgcg aggcgattgc
      901 ggcggtgcag aagtaccagc gcctgatcag cgacggcggg cgctttcaga tggtggcggg
      961 cgcgcgcgcc agctggctga tctaccaccc gcgccagggc acgctggcct tcagcccgca
     1021 ccaggtggag gcggtgctgg ccaccgtgct ggcctttgtg cgcaatgcac tgggccagcg
     1081 cccgggcgcg ggtgtgcggc cgctgcaggt gcagttcagc cagccgcggg tggggccgct
     1141 ggccggctac cgcgaggtgt ttgcctgccc gctggcgttc gagcaggcct tcaacggcct
     1201 gctgctggac aacgccgtgc tggacgcgcc gctgccgcat gccgacccgc accgcgcccg
     1261 cgcgcaccag cgcgaagcgg cggcgcatct ggccggcctg agccagggcg gggcgctggc
     1321 gcaggagctg cgcgcctgga tggctgcggc gctggccagc cgcgtgccca cgcgggccga
     1381 ggccgccgcc gcgctgggcg tgagcgaacg caccctggcg cgccgcctgc gggcgcaggg
     1441 catcagcttt gccgagctgc tggacggcgt gcggcgcgac gcggcgctgc aggccgttgg
     1501 gcagaccacg cgcccgctgg ccgacatcgg ccaggccctg ggctttgccg agccagcggt
     1561 gttctggcgc gccttcaagc gctggagtgg cagcacgccc gccgcctggc gcgcggcgca
     1621 gcgcgctgat cgccagccct gacgaatgag ctcgctgcgg ttcgcatgac caatgtcaac
     1681 ggcgccatcc ccttttgtca ttgatgccgc gcagcggcga agtcattcgt catgccaacg
     1741 ccacgcggca ggggcgttgc actggcgtgg cttgccgcac ttatgcaccc caactgtggg
     1801 ccgggctgtg gaaaaccccc tgaacaagcc gcgcaagccg cgccggtatt gggctggcgg
     1861 gcgctgcctg cggattgagc agagggtcga gccggtggcg gggcgagcac agggttcagc
     1921 tgaaaatgct accaatagaa tagctgttgg cgcttcactg acaagcgctg gcgcccgatt
     1981 tggcttgtaa ccggacgggc gcggccaagc gcccgcctca gatgtgcagc gcctgcccca
     2041 gcgcgcgcag cgccgcttcc tgcaccgctt cgcccagcgt cgggtgggcg tggatggtgt
     2101 ggcctacgtc ttccagccgc gcgcccatct cgatcgactg gctgaaggcg gcggccagtt
     2161 cggccacgcc cttgcccacg gcctgccagc ccaggatcag gtggttgtcg cggcgcgcca
     2221 ccacgcgcac gaagccgctg gcagcctcca gcgtcatggc gcggccgttg gcggcgaacg
     2281 ggaaggcggc gtcgatgcag tccatgccgg cggcgcgggc ctcgccgggc gtcttgcccg
     2341 tcaccaccac ctcggggtcg gtgaagcaca cggcgggaat ggccatgggc tcgaagcgcc
     2401 ggttc
//
LOCUS       DAOQTZ010000198         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_56293_length_2343_cov_13.293269, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000198 DAOQTZ010000000
VERSION     DAOQTZ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56293_length_2343_cov_13.293269"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..715
                     /locus_tag="PKN51_13740"
     CDS             335..715
                     /locus_tag="PKN51_13740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNT86206.1"
                     /translation="MHHPFADLIDLKVEVQRAGHSQLSLTLAPQHLNPHGVVHGAVVF
                     ALADTGMGSALYPTLEPGQICATIDIAISYFRPVRAGRLVCLTEVVNRGRTVAHLSSR
                     VLAGEQLVASATGNYAIFVPREAG"
     gene            1055..>2343
                     /locus_tag="PKN51_13745"
     CDS             1055..>2343
                     /locus_tag="PKN51_13745"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HNT86207.1"
                     /translation="MTRSYAAEPFKIKVVEPIKMTTRAERAQYLAEAGYNTFLLRSDQ
                     CYIDLLTDSGTTAMSDYQWAGMMLGDEAYAGSKNFIHLQQVVREYYGFRHIVPTHQGR
                     GAENLLSRLCIQPGDTVPGNMYFTTTRAHQELNGARFVDIIIDEAHDPQIELPFKGNV
                     DIAKLSRLIDEVGPERIPYVCVAVTVNLAGGQPVSMANLRAVKALCARHGIRVMFDAT
                     RCVENAYFIQEREPGYADKTIRAILKEMMSHADGCTMSGKKDCLVNIGGFLCMNDDEL
                     YQRASELVVLFEGMPSYGGLAGRDMEAMARGIEESVDQHYISHRVGQCRYLAEQLEAA
                     GIPIVKPVGGHAVFLDARAFLDHLPQDDFPAQTLAAQLYLESGVRSMERGIVSAGRDK
                     ETGDHHRPKLELVRLTIPRRVYTYAHLDVVARACKDL"
BASE COUNT          411 a          829 c          728 g          375 t
ORIGIN      
        1 acgcactgga tctggttcat cctgccgcag ctgcggggcc tgggcaccag cccgcggcgt
       61 cgtcgcgtct tgcctggcac gccgatcacg gcacgggcgg gtacgtccca ctgccgtttc
      121 taggctgtgt ggcgcggggg ccatgcgcca tggcaccggc gggtgcggcc agtcttcacg
      181 atcattccag gtgttttgag gctccagcgc ttgctggttg tgcgccagca gctatcaaaa
      241 acatagtgtt cggtgcctcg gctatgctgc catgtgtcgc gccggggcgg ctcgcgcccg
      301 ccggcatcgt gtctgacttc tgcccctgtc caccatgcac catccctttg ccgacctgat
      361 cgatctgaag gtggaggtgc agcgcgccgg ccacagccag ctcagcctga cgctggcgcc
      421 gcagcacctg aacccgcatg gcgtggtgca tggcgcggtg gtgttcgcgc tggccgacac
      481 cggcatgggg tcggcgctct accccacgct ggagccaggg cagatctgcg ccaccatcga
      541 catcgccatc agctacttcc gccccgtgcg ggccggccgg ctggtgtgcc tgaccgaagt
      601 ggtcaaccgc ggccgcacgg tggcgcacct gagctcgcgc gtgctggcgg gcgagcagct
      661 ggtggcctcg gccaccggca actacgccat cttcgtgccg cgcgaggcgg gctgagccgc
      721 tggccgtgcc gggcgcggca tccatgggcg tttcagggtg ctggggcttt tctggcgtgc
      781 gccagcagct attgttttca tagtcattcg ggggccttgg ggcgcccggc ggcatgccgt
      841 acccctgcgc atggtgcggt caccaaagca tgaaaagtgc gtaaacatga aaacaatgac
      901 agaaaacatc aaccagcaag gcctcaaata cggcttctgt tgcgtcggtt ggcgtttgaa
      961 agcagaaaag cttgattcgg atcaagctac tgcccggtca cccggcccgc cgccacgcac
     1021 gccttgttcc accgccctcg ccaggagaca cgccatgacc cgcagctacg ccgccgaacc
     1081 cttcaagatc aaagtggtcg agccgatcaa gatgaccacg cgcgccgagc gcgcccagta
     1141 cctggccgag gccggctaca acaccttcct gttgcgctcc gaccagtgct acatcgacct
     1201 gctgaccgac agcggcacca ccgccatgag cgactaccag tgggccggca tgatgctggg
     1261 cgacgaagcc tacgccggca gcaagaactt catccacctg caacaggtgg tgcgcgagta
     1321 ctacggcttc cggcacatcg tgcccaccca ccaggggcgc ggcgccgaaa acctgctgtc
     1381 gcgcctgtgc atccagccgg gcgacacggt acccggcaac atgtacttca ccaccacgcg
     1441 cgcccaccag gagctcaacg gcgcccgctt cgtcgacatc atcatcgacg aggcgcacga
     1501 cccgcagatc gagctgccgt tcaagggcaa cgtcgacatc gccaagctca gccgcctgat
     1561 cgacgaggtt gggcccgagc gcatccccta cgtctgcgtc gccgtcaccg tcaacctggc
     1621 cggcggccag ccggtcagca tggccaacct gcgcgccgtc aaggcgctgt gcgcgcgcca
     1681 cggcatccgc gtgatgttcg acgccacgcg ctgcgtcgaa aacgcctact tcatccagga
     1741 gcgcgagccg ggctatgccg acaagaccat ccgcgccatc ctgaaagaga tgatgagcca
     1801 tgccgacggc tgcaccatga gcggcaagaa ggactgcctg gtcaacattg gcggctttct
     1861 gtgcatgaac gacgacgagc tgtaccagcg cgccagcgag ctggtggtgc tgttcgaggg
     1921 catgcccagc tacggcggcc tggccgggcg cgacatggaa gccatggcgc gcggcatcga
     1981 agaatcggtc gaccagcact acatcagcca ccgcgtcggc caatgccgct acctggccga
     2041 gcagctcgaa gccgccggca tccccatcgt caagccggtc ggcggccacg ccgtgttcct
     2101 ggacgcgcgc gccttcctcg accacctgcc gcaagacgac tttccggccc agaccctggc
     2161 cgcccagctg tatcttgaaa gcggcgtacg cagcatggag cgcggcatcg tctcggccgg
     2221 ccgcgacaag gaaaccggcg accaccaccg ccccaagctc gaactggtgc gcctgaccat
     2281 cccgcgccgc gtctacacct acgcccacct cgacgtggtg gcgcgcgcct gcaaggacct
     2341 gta
//
LOCUS       DAOQTZ010000199         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_66895_length_2102_cov_13.627748, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000199 DAOQTZ010000000
VERSION     DAOQTZ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66895_length_2102_cov_13.627748"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKN51_13750"
     CDS             <1..648
                     /locus_tag="PKN51_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNT86208.1"
                     /translation="RMIEQYSRQAGFLLEEGATPQQVDKAVEKFGFAMGPFRMGDLAG
                     NDIGWAIRKRRYVEKPDMKYSKTADLLCELGRYGQKTGAGWYDYQPGKRDAIPSKLVE
                     DMIAKHRQEQGITPRKISDEEIVQRLVYALVNEGARILEDGIASKASDIDMVYLTGYG
                     FPIWRGGPMKYAEQVGLFNVVESMKRFARNPLDDARFWQPAPLLSKLVAEGRNFS"
     gene            707..1882
                     /locus_tag="PKN51_13755"
     CDS             707..1882
                     /locus_tag="PKN51_13755"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNT86209.1"
                     /translation="MTSAVIVSTARTPLAKSWKGAFNMTHGATLGGHAVQHAIARAGI
                     DAAQVEDVIMGCANPEGATGMNIARQIALMAGCPISVCGMTVNRFCSSGLQTIALAAQ
                     RIIAGEGTTFVAGGVESISCVQQEMNMHMIQDPALLARKPEIYWSMLQTAEQVAKRYG
                     IGRDVMDEYGAASQQKACAAQAAGKFDDEIAPITVTMGVADPVRGLSTRQVTCSKDEG
                     TREGTTKEGISGIRPALPGGLVSAGNASQFSDGAGACVLMHESEASKQGLKPIGRFLG
                     FAVAGCEPDEMGIGPVYAVPKVLQRLGLKVGDIDLWELNEAFAVQVLYCRDKLGIPAD
                     RLNVNGGAIALGHPYGVSGQRLTGHALIEGKRRGAKRVCVTMCIGGGMGAAGVFEVL"
BASE COUNT          418 a          675 c          685 g          324 t
ORIGIN      
        1 cgcatgatcg agcagtacag ccgccaggcc ggcttcctgc tggaagaggg cgccaccccg
       61 caacaggtcg acaaggcggt cgagaagttc ggctttgcca tgggtccatt ccgcatgggc
      121 gatctggccg gcaacgacat cggctgggcc atccgcaagc gccgctacgt cgaaaagccc
      181 gacatgaagt acagcaagac cgccgatttg ctgtgcgaac tgggccgcta cggccaaaaa
      241 accggcgccg gctggtacga ctaccagccc ggcaagcgtg acgccattcc cagcaaactg
      301 gtcgaggaca tgatcgccaa gcaccgccag gagcagggca tcacaccgcg caagatcagc
      361 gacgaagaga tcgtgcagcg tctggtgtac gcgctggtca acgaaggcgc acgcattttg
      421 gaagacggca tcgccagcaa ggccagcgac atcgacatgg tgtatctgac cggctatggc
      481 tttccgatct ggcgcggcgg cccgatgaaa tacgccgagc aggtgggcct gttcaacgtg
      541 gtggaaagca tgaagcgctt tgcgcgcaac ccgctggacg acgcccgatt ctggcaaccc
      601 gcgccgctgc tgagcaagct cgtcgccgaa ggcaggaatt tcagctgatt tcgcggccca
      661 gccattgatc agtcagcgcc aacagctatc gaatcaggag taattcatga cctctgccgt
      721 gatcgtatcc accgcccgta ccccgcttgc caagagctgg aagggcgctt tcaacatgac
      781 ccatggcgcc accctgggcg gccatgcggt gcaacacgcc atcgcccgcg cgggcatcga
      841 cgccgcgcag gtggaagacg tcatcatggg ctgcgccaac cccgaaggcg ccaccggcat
      901 gaacatcgcg cgccagatcg cgctgatggc cggttgcccg atcagcgtct gcggcatgac
      961 ggtgaaccgt ttctgttcgt cgggcctgca gaccatcgcc ctggcggcgc agcgcatcat
     1021 cgccggagaa ggcaccacct tcgtcgccgg cggcgtggag agcatctcct gtgtccagca
     1081 ggaaatgaac atgcacatga tccaggatcc ggcgctgctc gccaggaagc ccgagatcta
     1141 ttggagcatg ctgcaaaccg ccgagcaggt ggccaagcgc tacggcatcg gccgcgacgt
     1201 catggacgaa tacggcgccg ccagccagca gaaggcctgt gccgcgcagg ccgcgggcaa
     1261 gttcgacgac gaaatcgccc ccatcaccgt caccatgggt gtggccgatc cggtgcgcgg
     1321 cctgagcacg cgacaggtca cctgcagcaa ggacgaaggc acgcgcgagg gcaccaccaa
     1381 ggaaggcatc agcggcatca ggccggcgct gcccggtggt ctggtttcgg ccggcaacgc
     1441 cagccagttc agcgacggcg ctggcgcctg cgtgctgatg cacgaaagcg aagccagcaa
     1501 acagggtctg aaaccgatcg gccgctttct ggggtttgcc gtggccggtt gcgaacccga
     1561 cgaaatgggc atcggcccgg tctatgccgt gcccaaggtg ctgcaacgtc tggggttgaa
     1621 ggtcggcgac atcgacctgt gggagctcaa cgaagccttc gccgtacagg tgctgtactg
     1681 ccgcgacaag ttgggcattc cagccgatcg gctgaacgtc aacggcggcg cgattgcgct
     1741 gggccatccg tacggcgtca gcggccagcg cctgaccggg cacgccctga tcgaaggcaa
     1801 gcgccgcggc gccaagcgcg tgtgcgtgac catgtgcatt ggcggcggca tgggtgcggc
     1861 cggcgtgttc gaagtgctgt gatttgaccg gaaactggcg ctcccgttgc gccgccagcg
     1921 ccccgcaccg gacgatcggt gcggggcgct ttcgtgtgcg ccaccgttcc agcagcggtg
     1981 cggcggctca atccggacga cagcgaaagt ctgacgcgag tccaccacca cgatggttta
     2041 acctagaaac ggcagttgac cgctggtcaa gttgaccgct ggtcaacttc gggcagaccg
     2101 ca
//
LOCUS       DAOQTZ010000200         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_68278_length_2075_cov_14.400000, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000200 DAOQTZ010000000
VERSION     DAOQTZ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68278_length_2075_cov_14.400000"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..804
                     /locus_tag="PKN51_13760"
     CDS             58..804
                     /locus_tag="PKN51_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNT86210.1"
                     /translation="MATRYKYLTARNTALLGALLEGLRNAGYLRIYGWIGVGILLVTL
                     LLTLFDRHFDPQFPRRWRDGLAESFYAAVKIATAGQVPGRTNRLGWLGRVWQGVWLLP
                     AIALVAFVTSSVTSVLTTQSLHHQINSLQDVRDQPVGVLAGTVEEDYGRTQGLTLRSY
                     DDLDQASAALLAGEVQAVMADAPMLEYHAHTHPELPVKVVGRLFNRDRYGFALPLNSP
                     RTRDITLEVLKAVESGQVEQLRLRYFGRNP"
     gene            complement(827..1987)
                     /locus_tag="PKN51_13765"
     CDS             complement(827..1987)
                     /locus_tag="PKN51_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT86211.1"
                     /translation="MSFVLNRRRFSAGLALAPLATGGALSGCSQIPDRVKIGVAQPLT
                     GPIHELGKDLLNGVQLAVTELNAANYTINGKRVTLEVVAVDDKSDAETGKAVAQQLVE
                     AGVVAVIGHLNSGVSIAAAPIYAAQGMAQIAISTNPKFTELGLDTTFRMVANDHMQAK
                     AMGSYAGAQFGDVPYAAVDDGTPYGKGLIEGAARELKARKREIVLQQSFDDKTVAFDD
                     LAARIKAAKVGVIVSTLNDFQVIALIEALRKVNHTDVRMLGGDTLKTTGMLKARGTIR
                     GIHATSPVLEAKEFLSGRAFLSRYTSAFKIAPAYGGHYTYDSMHVLADAIQQAKSVKP
                     RDVTAVLRRISGSAPVTGSMRWDDKGEQRYAAVGVYDLGRDGWNLRIRSDTW"
BASE COUNT          334 a          704 c          674 g          363 t
ORIGIN      
        1 gcgtggcctt gacatgggtc caccatgcct gcggccacgc gccgcgcagc tggccccttg
       61 gcgactcgct ataagtacct gacagcgcga aacactgcac tactcggtgc cctgctggaa
      121 ggcctgcgca acgccggcta cctgaggatc tatggctgga tcggggtcgg cattctgctg
      181 gtcacgctgc tgctgacatt gttcgaccgg cacttcgacc cgcagtttcc gcgccgctgg
      241 cgcgacggcc tggccgagag cttctacgcc gccgtgaaaa tcgccaccgc cggccaggtg
      301 cctgggcgaa ccaaccgcct gggctggctg ggccgcgtct ggcagggcgt gtggctgctg
      361 ccggccatcg ccctggtggc tttcgtcacc tcatcggtca ccagcgtgct gaccacccag
      421 tcgctgcacc accagatcaa cagcctgcag gatgtgagag atcaacccgt gggcgtgctc
      481 gccggcaccg tggaggagga ctacggccgc acgcagggcc tgacgttgcg cagctacgac
      541 gacctcgatc aggccagcgc agccctgctg gcgggcgagg tgcaggcggt catggccgat
      601 gcgccgatgc tcgagtacca cgcccacacc cacccggaac tgccggtcaa ggtggtgggc
      661 cgcctgttca accgcgaccg ctatggcttt gcgctgccgc tgaacagccc acgcacgcgc
      721 gacatcacgc tggaggtgct gaaggcggtc gaatcgggcc aggtcgaaca gctgaggctg
      781 cgctacttcg gtcgcaaccc gtgaccgggc agcggccccg gccgcgctac caggtgtccg
      841 agcggatgcg caggttccag ccgtcgcgcc ccaggtcgta gacaccgacg gcagcgtagc
      901 gctgctcgcc cttgtcgtcc cagcgcatgg atccggtgac cggggccgag ccgctgatgc
      961 ggcgcagcac ggccgtcacg tcgcgcggct tgaccgactt ggcctgctgg atggcatcgg
     1021 ccagcacgtg catgctgtcg taggtgtagt gcccgccgta ggccggagca atcttgaacg
     1081 cgctggtgta gcggctcaga aatgcccgtc ccgacagaaa ctccttggcc tccagcaccg
     1141 gcgatgtggc gtggatgccg cggatcgtcc cgcgcgcctt gagcatgccg gtggttttga
     1201 gcgtgtcgcc gcccagcatg cgcacatcgg tgtggttgac cttgcgcagc gcctcgatca
     1261 acgcgatgac ctggaagtcg ttgagcgtgg agacgatcac ccccaccttg gccgccttga
     1321 tccgggccgc caggtcatcg aaggccaccg tcttgtcgtc aaacgactgc tgcagcacga
     1381 tctcgcgctt tcttgccttc agttcgcgcg ccgctccttc gatcagcccc ttgccatagg
     1441 gggtgccgtc gtcgaccgcg gcgtagggca cgtcgccgaa ctgggcgccc gcataggaac
     1501 ccatggcctt ggcctgcatg tgatcgttgg ccaccatgcg gaaggtggtg tccaggccca
     1561 gctcggtgaa cttcgggttg gtggaaatgg cgatctgggc catgccctgg gcagcgtaga
     1621 tcggtgcggc agcgatgctg acgcccgagt tgagatgacc gatcacggcc accacgccgg
     1681 cctccaccag ctgctgcgcc accgccttgc cggtctcggc gtccgatttg tcgtccacgg
     1741 caacgacttc cagcgtcacg cgtttgccgt tgatggtgta gttggccgcg ttcagctcgg
     1801 tcaccgccag ctgcaccccg ttgagcaggt ccttgcccag ttcgtggatc ggccctgtca
     1861 acggctgtgc cacgccgatc ttgacccgat cgggaatctg gctgcagccg ctcagcgcgc
     1921 cgccggtggc aagcggcgcc agtgccaggc cggcggagaa ccgtcgacgg ttgaggacaa
     1981 aggacatgac ttcactcctg ataagactgg ccagaatcac gcaccacccg cgctccgctg
     2041 cgccacgggc agatcgaatc aacaatataa aagta
//
LOCUS       DAOQTZ010000201         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_72402_length_2000_cov_13.456555, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000201 DAOQTZ010000000
VERSION     DAOQTZ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72402_length_2000_cov_13.456555"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKN51_13770"
     CDS             <1..310
                     /locus_tag="PKN51_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT86212.1"
                     /translation="AGLLSHGQKQWLEIGMLLAQEPKLLLLDEPVAGMTDEETVRTAE
                     LFLSLKGKHSLMVVEHDMSFIETISEKVTVLCDGAVLAEGTLAQVQADERVIEVYLGR
                     "
     gene            364..1056
                     /gene="urtE"
                     /locus_tag="PKN51_13775"
     CDS             364..1056
                     /gene="urtE"
                     /locus_tag="PKN51_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HNT86213.1"
                     /translation="MLKVDNLHQYYGGSHILRNVSLRAEVGKVTVLLGRNGVGKTTLL
                     KSLMGLVPIKSGSLQWQGQDIARKTPYERARAGIGYVPQGREIFARLTVEENLRMGLA
                     YCPARTAIPAELYELFPVLKQMLHRRGGDLSGGQQQQLAIARALAPGPRLLILDEPTE
                     GIQPSIIKDIGRVIRMLAGRGDMAILLCEQYYDFAEELADHYLVMERGEVIAAGPGSE
                     MQQKEIRKLVAI"
     gene            1189..>2000
                     /locus_tag="PKN51_13780"
     CDS             1189..>2000
                     /locus_tag="PKN51_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HNT86214.1"
                     /translation="MGWHAQLALDYRACHGRTVLHFTHDGPLRVLKSLYPEGPAICHN
                     VLVHPPGGLVGGDELDITVQVGAGAHALVTTPGAARFYKSNGQPARQRTRLRLAAGAR
                     LEWLPLEAIAYNACDAHNHLEFELAEGAQMLAWDITALGLPLAGQAFALGRLTQHLEW
                     PGVYLERGLIDAQDGLLLDGDLGLAGQRCLASLVLASGTPLPRSTREQLLEDMHALLQ
                     CGHGSVLAGVTAPNDHMLVLRALAPVVEPAMALCRSAWSLWRGQLWGLRGDMP"
BASE COUNT          348 a          651 c          696 g          305 t
ORIGIN      
        1 ggccggcctg ctgagccacg gccagaagca gtggctggag atcggcatgc tgctggcgca
       61 ggagcccaag ctcttgctgc ttgacgagcc ggtggcgggc atgaccgacg aggaaaccgt
      121 gcgcacggcc gagctgtttc tgtcgctcaa gggcaagcat tcgctgatgg tggtggagca
      181 cgacatgagc ttcatcgaaa ccatcagcga gaaggtcacg gtgctgtgcg acggcgcggt
      241 actggccgag ggcacgctgg cccaagtcca ggccgacgag cgtgtgatcg aggtttatct
      301 gggccgataa actatcaatg taatagctgc tcgcgcttga tacacaaggg ctggaggcca
      361 aaaatgctta aagtcgacaa cctgcaccag tactacggcg gctcgcacat tctgcgcaac
      421 gtcagcctca gggccgaggt cggcaaggtc acggtgctgc tgggccgcaa cggcgtgggc
      481 aagaccacgt tgctcaagag tctgatgggg ctggtgccga tcaagagcgg cagccttcag
      541 tggcaaggcc aggacatcgc gcgcaagacg ccctatgaac gcgcccgcgc cggcatcggc
      601 tacgtgccgc aggggcgcga gatctttgcg cgcctgacgg tcgaggaaaa tctgcggatg
      661 ggactggctt actgccccgc gcgcaccgcc atcccggccg agctgtacga gctgttcccg
      721 gtgctcaaac agatgctgca ccgccgcggc ggcgatcttt cgggcgggca gcagcagcag
      781 ctggcaattg cccgcgcgct ggcgccgggc cccaggctgc tgatcctgga cgagccgacc
      841 gagggcatac agcccagcat catcaaggac atcggccgcg tgattcgcat gctggccggg
      901 cgcggcgaca tggccatcct gctgtgcgag caatactatg actttgccga agagttggcc
      961 gaccactatc tggtgatgga gcgcggcgaa gtgattgccg ccgggccggg cagcgaaatg
     1021 cagcaaaagg aaatccgcaa actggtggcc atctgaaatc gcccaagcaa ccctgcatgc
     1081 gcatgctgac caccccgatg ccaccgccta gccttgcgca ggcgcgccgc agtctgcgcg
     1141 ggggtgacgg cgcggcgcta cggcggtcca cgcacggctt ctgaatccat gggctggcat
     1201 gcgcaactgg cgctcgacta tcgcgcatgc cacggcagaa ccgtgctgca cttcacgcac
     1261 gacggcccgc tgcgcgtgct caagagcctg taccccgaag gcccagccat ctgccacaac
     1321 gtgctggtgc acccgcccgg cgggctggtc ggcggcgacg agctcgacat cacggtgcag
     1381 gtgggggccg gcgcccatgc gctggtgacc acgccgggcg cagcgcgctt ctacaaaagc
     1441 aacggccaac cggcgcgcca gcgcacgcgg ctgcgcctgg ccgccggggc gcggcttgaa
     1501 tggctgccgc tggaggccat tgcctacaac gcctgcgacg cgcacaacca ccttgaattt
     1561 gagctggccg aaggcgcgca gatgctggcc tgggacatca ccgcgctggg cctgccgctg
     1621 gctggtcagg ccttcgcgct gggccgcttg acgcagcacc tggagtggcc gggggtgtat
     1681 ctggagcgcg gcctgatcga cgcgcaggat ggcctgctgc tcgatggcga cctgggcctg
     1741 gccggccagc gctgcctggc cagcctggtg ctggccagcg gcaccccact gccgcgttcg
     1801 acgcgcgaac aactgctgga ggacatgcac gcgctgctgc aatgcgggca cggcagcgtg
     1861 ctggccggcg tgaccgcgcc caacgatcac atgctggtgc tgcgcgcgct ggcgccagtg
     1921 gtggagccgg ccatggccct gtgcaggagc gcctggtccc tgtggcgcgg ccagctgtgg
     1981 ggcctgcgcg gcgacatgcc
//
LOCUS       DAOQTZ010000202         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_77342_length_1919_cov_16.480687, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000202 DAOQTZ010000000
VERSION     DAOQTZ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77342_length_1919_cov_16.480687"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1085
                     /locus_tag="PKN51_13785"
     CDS             111..1085
                     /locus_tag="PKN51_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HNT86215.1"
                     /translation="MTTRRHFNASLIAAGAALASPLVRANDDAAIHVLVGFPAGGGSD
                     VIARLLAEKLQAELGRSVVVDNRPGAGGQIAAQQLKAARPDGTTVFLSHDHTISILPQ
                     VVRKPGYAPTEDFVALGGFATFVNGLAVSAATPANTLSEYVEWVRSRQGGKSAIGIPA
                     PASTPEFLVQLIGRKYGLALTAVPYKGSAPMIADMLGNQIAAGTGSVQDFIEYQRAGK
                     LRVIGVLGGKRQAALPDVPTFSEMGFGGLEDTPFYGLWAPRGTPAAFIDRFTRALDKV
                     VALPEVRARLTDLGLTVGYMTPQQLDQRERAYTKVWSRIIADSGFQPQ"
     gene            complement(1161..1841)
                     /locus_tag="PKN51_13790"
     CDS             complement(1161..1841)
                     /locus_tag="PKN51_13790"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNT86216.1"
                     /translation="MGTLYLVRHGQASFGADNYDLLSELGRAQSLRLGQYWRERFGAE
                     LAFDAVLTGTLQRQRQTWQGIVEGAQLSGVPHVEWPGLNEYDSHAVIRAIHPDPLPPP
                     DTPERYRLHFRLLRSGLAAWMRGEVQPAGMPSYADFVRGVTSALEHVRTHYPGQVLLV
                     SSGGPISTAVGQLLSTPAETTIELNMRYRNSAVTELSFTPRRHQLLSYNVLPHLDEAR
                     YRDWVTYA"
BASE COUNT          294 a          673 c          673 g          279 t
ORIGIN      
        1 cgcgccctgg ccgcgctgct gcaggagatc gaccgcctgc cggccgacac gctgcgccag
       61 cacagcctcc tagaatcgac cactttgcca accaccacgg acgacagccc atgaccacgc
      121 gccgccactt caacgcctcc ctgatcgccg ccggtgccgc gctggcctcg cccctggtgc
      181 gggccaacga cgacgcggcc atccacgtgc tggtgggttt tccggccggc ggcggctccg
      241 acgtcatcgc ccgcctgctg gccgagaaac tgcaggccga actgggccgc agcgtggtgg
      301 tggacaaccg tcccggcgcc ggcggccaga tcgcggcgca gcagctcaag gcggcgcggc
      361 cggacggcac cacggtgttc ctgtcgcacg accacaccat ctcgatcctg ccgcaggtgg
      421 tgcgcaagcc cggctacgca ccgaccgagg acttcgtggc gctgggcggc tttgccacct
      481 tcgtcaacgg gctggcggtg tcggcggcga cgccggccaa cacgctgtcc gagtacgtcg
      541 aatgggtgcg cagcaggcag ggcggcaaga gcgccatcgg catcccggcg ccggcctcga
      601 cgccggaatt tctggtgcag ctgatcggca ggaaatacgg cctggcgctg accgccgtgc
      661 cctacaaggg cagcgcgccg atgatcgccg acatgctggg caaccagatt gcggccggca
      721 ccggctcggt gcaggacttc atcgaatacc agcgcgccgg caagctgcgc gtgatcggcg
      781 tgctgggcgg caagcgccag gcggcgctgc ccgacgtgcc caccttcagc gaaatgggct
      841 tcggcgggct ggaggacacg ccgttctacg gcctgtgggc gccgcgcggc acgccggccg
      901 ctttcatcga ccgcttcacg cgcgcgctcg acaaggtggt ggcgctgccc gaggtgcgcg
      961 cgcgcctgac cgacctgggc ctgacggtgg gctacatgac gccgcagcag ctcgaccagc
     1021 gcgaacgcgc ctacaccaag gtgtggtcgc gcatcatcgc cgacagcggc tttcagcccc
     1081 agtgatcttt tggaccaata aggcctgaaa accgcttaca tcaagcgccg gcagctacaa
     1141 tttcagtagc gattggggtc tcaggcgtag gtcacccagt cgcggtagcg ggcctcgtcc
     1201 aggtgcggca gcacgttgta gctgagcagc tggtggcgcc gcggcgtgaa gctcagctcg
     1261 gtcacggccg agttgcggta gcgcatgttc agctcgatgg tggtttcggc cggggtggac
     1321 agcagctgcc ccaccgccgt gctgatcggc ccgccgctgg acaccagcag cacctggccc
     1381 gggtagtggg tgcgcacatg ctccagcgcg ctggtgacgc cgcgcacgaa atccgcgtag
     1441 ctgggcatgc cggccggctg cacctcgccg cgcatccagg ccgccaggcc gctgcgcagc
     1501 aggcgaaagt gcaggcggta gcgctcgggc gtgtcgggcg gcggcagcgg gtcggggtgg
     1561 atggcgcgga tcaccgcgtg gctgtcgtac tcgttcagtc ccggccactc gacgtggggc
     1621 acgccgctga gctgcgcgcc ttcgacaatg ccctgccagg tctgccgctg gcgctgcagg
     1681 gtgccggtga gcacggcgtc gaacgccagc tcggcgccaa agcgctcgcg ccagtactga
     1741 cccagccgca gcgactgggc gcgaccgagc tcgctcagca ggtcgtagtt gtcggcgccg
     1801 aagctggcct gcccgtggcg cacgaggtaa agggttccca tggcgcattc ttgcccgccc
     1861 ggcggcgcgc cggcgtcaaa ccggcgacag cgccggtcat cctagaaacg gcagttgga
//
LOCUS       DAOQTZ010000203         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_1127
            NODE_80408_length_1874_cov_14.667400, whole genome shotgun
            sequence.
ACCESSION   DAOQTZ010000203 DAOQTZ010000000
VERSION     DAOQTZ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852470
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:16:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,761
            CDSs (total)                      :: 2,733
            Genes (coding)                    :: 2,715
            CDSs (with protein)               :: 2,715
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80408_length_1874_cov_14.667400"
                     /isolate="AMR_MDS_1127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKN51_13795"
     CDS             complement(<1..338)
                     /locus_tag="PKN51_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNT86217.1"
                     /translation="MALMDFIKKQFIDILEWTESGDGTLAWRFPMQDMEIQTGAALVV
                     RESQMALFVNEGQVADVFGPGTYKLSTQTLPVLTYLKNWDKLFASPFKSDVYFFSTRQ
                     QVDQKWGTPQP"
     gene            501..746
                     /locus_tag="PKN51_13800"
     CDS             501..746
                     /locus_tag="PKN51_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HNT86218.1"
                     /translation="MPRLIRTHLPTLKKTASYYVMHIAVAAAVAWLVTGDLLMALTLS
                     LLEPTVQAVAFFFHEKAWARVDGRSAAPAAAGPALGA"
     gene            922..>1874
                     /locus_tag="PKN51_13805"
     CDS             922..>1874
                     /locus_tag="PKN51_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNT86219.1"
                     /translation="MTTTPPSQDDKREQLRQAALDYHEHPVPGKIAIAATKQLVNQYE
                     LSLAYSPGVAAPCEEIQRDPAAAFRYTARGNLVGVITNGTAVLGLGDIGALAGKPVME
                     GKAVLFKKFSGIDVFDIEINEKDPDKLVEIIAALEPTFGGINLEDIKAPECFTVEARL
                     RERLKIPVFHDDQHGTAIVVGAAVVNGLKVVGKKIEDVKLVVSGAGAAALACTGLLVD
                     LGVRRENVWVTDIEGLVYEGRTALMDEYKARYAQRTDKRTLAEVIEGADVFMGLSAGG
                     VLKPEMVARMAPRPLILALANPNPEILPEQVKAVRDDAVIAT"
BASE COUNT          325 a          635 c          628 g          286 t
ORIGIN      
        1 ggctggggcg tgccccactt ctggtcgacc tgctggcgcg tgctgaagaa gtagacgtcg
       61 ctcttgaagg gcgaggcaaa cagcttgtcc cagttcttca gataggtcag caccggcagc
      121 gtctgcgtgc tcagcttgta ggtgccgggg ccgaacacgt cggccacctg accttcgttg
      181 acgaacagcg ccatctgcga ctcgcgcacc accagggcgg cgccggtctg gatctccatg
      241 tcctgcatcg ggaagcgcca ggccagcgtg ccgtcgccgc tctccgtcca ctccaggatg
      301 tcgatgaact gcttcttgat gaaatccatc agtgccatgg ccggagctcc tgcaacagaa
      361 agtcaacgaa gcgcgcatgt tagcgcccct gccgtcatcc gtgccacacc ctgtgggcgc
      421 cggctatcgg gcgcataggc cgcatcgatt gccattcgta atgagaatca ttatcattga
      481 atgaactgcc gggagtcccc atgccacgcc tgatccgtac ccacctgccc acgctcaaga
      541 agaccgccag ctactacgtg atgcacatcg cggtggctgc cgccgtggcc tggctggtga
      601 cgggtgacct gctgatggcg ctcaccctca gcctgctgga gcccaccgtg caggcggtgg
      661 ccttcttctt ccatgaaaag gcctgggcgc gcgtggacgg ccgcagcgcc gcgccggcgg
      721 cggccgggcc ggcgctcgga gcctgaacgc ggcgctcagc gccgccagcc gactgcgggc
      781 gggccgcacc cctgcgggtc tggcggtttc gacggccgtc gcctgccagg tgaactggct
      841 gcacgcccgc cgctgcctac aatgaccggc cgcctgccgt ggtgcgccac cggcagggcc
      901 agccactgca ggacgccgac catgaccacc acgccaccga gccaagacga caaacgcgag
      961 cagctgcgcc aggcggcgct ggactatcac gagcacccgg tgcccggcaa gatcgccatc
     1021 gcggccacca agcagctggt caaccagtac gagctgtcgc tggcctacag ccccggcgtt
     1081 gccgcgccct gcgaggagat ccagcgcgat ccggcggccg ccttcaggta caccgcgcgc
     1141 ggcaacctgg tgggcgtgat caccaacggc accgccgtgc tgggcctggg cgacatcggt
     1201 gcgctggccg gcaagccggt gatggaaggc aaggcggtgc tgttcaagaa gttctcgggc
     1261 atcgacgtgt tcgacatcga gatcaacgag aaggacccgg acaagctggt cgagatcatc
     1321 gccgcgctgg agccgacctt cggcggcatc aacctggaag acatcaaggc gcccgaatgc
     1381 ttcaccgtcg aagccaggct gcgtgagcgg ctgaagatcc cggtgttcca tgacgaccag
     1441 catggcaccg ccatcgtggt cggcgccgcg gtggtcaacg gcctgaaggt ggtgggcaag
     1501 aagatcgaag acgtcaagct ggtggtgtcc ggcgccggtg cggcggcgct ggcctgcacc
     1561 ggcctgctgg tggacctggg cgtgcggcgc gaaaacgtct gggtcaccga catcgagggc
     1621 ctggtgtacg agggccgcac ggcgctgatg gacgaataca aggcgcgcta cgcccagcgc
     1681 accgacaagc ggaccctggc cgaggtgatc gagggcgccg acgtgttcat gggcctgtcg
     1741 gccggcggcg tgctcaagcc cgagatggtg gcgcgcatgg caccgcgccc gctgattctg
     1801 gcgctggcca accccaaccc cgagatcctg cccgagcagg tcaaggcggt gcgcgacgac
     1861 gccgtcatcg ccac
//
